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Sample records for astatine 199

  1. Organic chemistry of astatine

    International Nuclear Information System (INIS)

    The paper surveys the investigations on the chemical behaviour of astatine in organic systems and deals with the preparation and identification of its organic compounds. A discussion is given on some of the physico-chemical properties of these compounds determined by extrapolation techniques as well as by direct measurement. The biomedical importance of 211At-labelled compounds is briefly referred to. (authors)

  2. Bibliography of astatine chemistry and biomedical applications

    International Nuclear Information System (INIS)

    An overall bibliography is presented on astatine chemistry and on the biomedical applications of its 211At isotope. The references were grouped in the following chapters: General reviews; Discovery, Natural Occurence; Nuclear Data; Preparation, Handling, Radiation Risk; Physico-chemical Properties; Astatine Compounds and Chemical Reactions; Biological Effects and Applications. Entries are sorted alphabetically by authors name in each chapter, and cross-references to other chapters are provided if appropriate. (R.P.)

  3. Astatine-211: production and availability.

    Science.gov (United States)

    Zalutsky, Michael R; Pruszynski, Marek

    2011-07-01

    The 7.2-h half life radiohalogen (211)At offers many potential advantages for targeted α-particle therapy; however, its use for this purpose is constrained by its limited availability. Astatine-211 can be produced in reasonable yield from natural bismuth targets via the (209)Bi(α,2n)(211)At nuclear reaction utilizing straightforward methods. There is some debate as to the best incident α-particle energy for maximizing 211At production while minimizing production of (210)At, which is problematic because of its 138.4-day half life α-particle emitting daughter, (210)Po. The intrinsic cost for producing (211)At is reasonably modest and comparable to that of commercially available (123)I. The major impediment to (211)At availability is attributed to the need for a medium energy α-particle beam for its production. On the other hand, there are about 30 cyclotrons in the world that have the beam characteristics required for (211)At production. PMID:22201707

  4. Recent advances in the organic chemistry of astatine

    International Nuclear Information System (INIS)

    Investigation on the chemical behaviour of astatine in the last decade are surveyed. The survey covers the physical and chemical properties of astatine, synthesis and identification of organic astatine compounds, their physicochemical properties. A special chapter is devoted to biomedical applications, including inorganic 211At species, 211At-labelled proteins and drugs. An extensive bibliography of the related literature is given. (N.T.) 129 refs.; 12 figs.; 14 tabs

  5. Dosimetrical considerations in astatine-211 radioimmunotherapy

    International Nuclear Information System (INIS)

    Several dosimetrical quantities have been suggested for use in alpha-particle dosimetry. To evaluate the expected biological effect when using these quantities, a Monte Carlo program was set to register the single-event distribution of both specific energy and alpha-particle track length to a cell nucleus (r=5.6 μm). Distributions were acquired for both 'bound' (simulating the effect of 211At-labelled antibodies bound to antigens on cell surfaces (r=7.0 μm)) as well as 'non-bound' (simulating 211At-labelled antibodies that have not bound to a cell) astatine-211. From these distributions, various theoretical cell survival curves were established for 3 different dosimetrical quantities, i.e. specific energy, number of alpha-particle hits and total track length. The survival curves for all quantities are presented for the corresponding mean absorbed dose in order to facilitate comparisons of the expected effects of using the 3 different quantities for both distributions of 211At decays. The theoretical survival curves presented here could, combined with experiments using 'bound' and 'non-bound' 211At in a single-cell suspension, reveal which dosimetrical quantity is most suitable for 211 At-radioimmunotherapy. (author)

  6. Discovery of the astatine, radon, francium, and radium isotopes

    OpenAIRE

    Fry, C; Thoennessen, M

    2012-01-01

    Currently, thirty-nine astatine, thirty-nine radon, thirty-five francium, and thirty-four radium isotopes have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  7. Extraction of astatine isotopes for development of radiopharmaceuticals using a 211Rn-211At generator

    International Nuclear Information System (INIS)

    In order to utilize a 211At isotope, a promising α-emitter for radionuclide therapy, the chemical properties of astatine isotopes are studied. We have examined wet chemistry methods through the distribution ratios of astatine in liquid-liquid extraction. The astatine isotopes have been found to be well extracted into DIPE and MIBK. We observed that the distribution ratio of astatine isotopes increases with concentrations of HCl greater than 3 M, while it decreases with the HCl concentration less than 2 M. The results will be useful for development of the 211Rn-211At generator. (author)

  8. Astatine-211-Labeled Targeted Radiotherapeutics: An Update

    International Nuclear Information System (INIS)

    The heavy halogen 211At was first proposed for use in α-particle targeted radiotherapy more than 30 years ago and continues to be one of the most promising radionuclides for this purpose. Although its 7.2-h half life is not ideal for intravenously administered whole antibodies, it is compatible with the pharmacokinetics of antibody fragments, peptides, aptamers and organic molecules. Its diverse chemistry allows its incorporation into a wide array of targeting vehicles, relying on its chemical similarity to iodine to provide a useful point of departure. On the other hand, the relatively low carbon-astatine bond strength is challenging. In common with the other α-emitters being discussed at this symposium, lack of reliable availability is one of the biggest hurdles in the use of 211At for targeted radiotherapy. However, in the case of 211At, it is not a question of production cost or availability of target material, because 211At can be produced in reasonable yield from natural bismuth targets. Rather, the difficulty is the lack of cyclotrons equipped with the medium energy α-particle beams required for its production. If the infrastructure for producing 211At is to be improved to the stage where 211At-labeled radiopharmaceuticals can have a meaningful impact, several developments must occur. First, the ability to produce clinically relevant levels of 211At that can be shipped to remote locations in chemically tractable form must be demonstrated. Approaches under consideration include compensating for radiolysis-mediated effects and the consideration of alternative chemistries. Second, strategies for compensating for heterogeneities in dose deposition must be developed, hopefully in a way that is compatible with approval for human use. And third, it is essential that more clinical trials be performed with 211At-labeled therapeutics, particularly in settings of minimum residual disease where the radiobiological advantages of α-particles can be best exploited. Our

  9. Scrutinizing "Invisible" astatine: A challenge for modern density functionals.

    Science.gov (United States)

    Sergentu, Dumitru-Claudiu; David, Grégoire; Montavon, Gilles; Maurice, Rémi; Galland, Nicolas

    2016-06-01

    The main-group 6p elements did not receive much attention in the development of recent density functionals. In many cases it is still difficult to choose among the modern ones a relevant functional for various applications. Here, we illustrate the case of astatine species (At, Z = 85) and we report the first, and quite complete, benchmark study on several properties concerning such species. Insights on geometries, transition energies and thermodynamic properties of a set of 19 astatine species, for which reference experimental or theoretical data has been reported, are obtained with relativistic (two-component) density functional theory calculations. An extensive set of widely used functionals is employed. The hybrid meta-generalized gradient approximation (meta-GGA) PW6B95 functional is overall the best choice. It is worth noting that the range-separated HSE06 functional as well as the old and very popular B3LYP and PBE0 hybrid-GGAs appear to perform quite well too. Moreover, we found that astatine chemistry in solution can accurately be predicted using implicit solvent models, provided that specific parameters are used to build At cavities. © 2016 Wiley Periodicals, Inc. PMID:27059181

  10. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    CERN Document Server

    Rothe, S; Antalic, S; Borschevsky, A; Capponi, L; Cocolios, T E; De Witte, H; Eliav, E; Fedorov, D V; Fedosseev, V N; Fink, D A; Fritzsche, S; Ghys, L; Huyse, M; Imai, N; Kaldor, U; Kudryavtsev, Yu; Köster, U; Lane, J; Lassen, J; Liberati, V; Lynch, K M; Marsh, B A; Nishio, K; Pauwels, D; Pershina, V; Popescu, L; Procter, T J; Radulov, D; Raeder, S; Rajabali, M M; Rapisarda, E; Rossel, R E; Sandhu, K; Seliverstov, M D; Sjödin, A M; Van den Bergh, P; Van Duppen, P; Venhart, M; Wakabayashi, Y; Wendt K D A

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of smallest quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the ionization threshold. The observed series of Rydberg states enabled the first determination of the ionization potential of the astatine atom, 9.317510(8) eV. New ab initio calculations were performed to support the experimental result. The measured value serves as a benchmark for quantum chemistry calculations of the properties of astatine as well as for the theoretical prediction of the ionization potential of super-heavy element 117, the heaviest homologue of astatine.

  11. Comment: 199 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available nal Institute for Basic Biology) licensed under CC Attribution2.1 Japan メダカ近交系 Hd-rR系統 南日本集団由来の純系。 メダカで最初にゲノム解読が行われた系統。 撮影:成瀬清(基礎生物学...研究所) Photo: Kiyoshi Naruse (National Institute for Basic Biology) bando 2009/09/30 11:23:24 2010/01/14 20:09:43 ... ...Japanese medaka Oryzias latipes Oryzias_latipes_L.png 199.png Kiyoshi Naruse (Natio

  12. Synthesis and Evaluation of Astatinated N-[2-(Maleimido)ethyl]-3-(trimethylstannyl)benzamide Immunoconjugates.

    Science.gov (United States)

    Aneheim, Emma; Gustafsson, Anna; Albertsson, Per; Bäck, Tom; Jensen, Holger; Palm, Stig; Svedhem, Sofia; Lindegren, Sture

    2016-03-16

    Effective treatment of metastasis is a great challenge in the treatment of different types of cancers. Targeted alpha therapy utilizes the short tissue range (50-100 μm) of α particles, making the method suitable for treatment of disseminated occult cancers in the form of microtumors or even single cancer cells. A promising radioactive nuclide for this type of therapy is astatine-211. Astatine-211 attached to tumor-specific antibodies as carrier molecules is a system currently under investigation for use in targeted alpha therapy. In the common radiolabeling procedure, astatine is coupled to the antibody arbitrarily on lysine residues. By instead coupling astatine to disulfide bridges in the antibody structure, the immunoreactivity of the antibody conjugates could possibly be increased. Here, the disulfide-based conjugation was performed using a new coupling reagent, maleimidoethyl 3-(trimethylstannyl)benzamide (MSB), and evaluated for chemical stability in vitro. The immunoconjugates were subsequently astatinated, resulting in both high radiochemical yield and high specific activity. The MSB-conjugate was shown to be stable with a long shelf life prior to the astatination. In a comparison of the in vivo distribution of the new immunoconjugate with other tin-based immunoconjugates in tumor-bearing mice, the MSB conjugation method was found to be a viable option for successful astatine labeling of different monoclonal antibodies. PMID:26791409

  13. Astatine-211: production, injection into monoclonal antibodies radiological effect, possible application to cancer treatment

    International Nuclear Information System (INIS)

    Methods developed in the Laboratory of Nuclear Problems, JINR, for producing astatine-211 and injecting it into monoclonal antibodies are described. The use of its diethylene triamine penta-acetic acid complex is shown to be the most effective method of injecting astatine into a biomolecules. The biological effect of the α-particles emitted from the astatine-211 is investigated using Chinese hamster fibroblasts and Ehrlich carcinoma cells. It is established that the mitotic activity depression, number of degenerating cells, number of cells with chromosome aberrations, and cellular surviving fraction depend on the concentration of the radionuclide in the medium 'in vitro'. The RBE of α-particles in comparison with 60Co γ-rays is 3. Injection of astatine-211 absorbed on tellurium particles into mice with ascitic tumors resulted in prolongation of their life or elimination of the tumors. (author). 39 refs, 7 figs

  14. Measurement of the first ionization potential of astatine by laser ionization spectroscopy

    OpenAIRE

    Rothe, S.; A. N. Andreyev; Antalic, S; Borschevsky, A.; Capponi, L.; Cocolios, T.E.; Witte, H.; Eliav, E.; Fedorov, D. V.; Fedosseev, V. N.; Fink, D. A.; Fritzsche, S.; Ghys, L.; Huyse, M.; Imai, N.

    2013-01-01

    The radioactive element astatine exists only in trace amounts in nature. Its properties can therefore only be explored by study of the minute quantities of artificially produced isotopes or by performing theoretical calculations. One of the most important properties influencing the chemical behaviour is the energy required to remove one electron from the valence shell, referred to as the ionization potential. Here we use laser spectroscopy to probe the optical spectrum of astatine near the io...

  15. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    Science.gov (United States)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom; Jensen, Holger; Palm, Stig; Lindegren, Sture

    2015-07-01

    To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical and physical properties for use in targeted therapies for cancer. Due to the very short range of the emitted α-particles, this therapy is particularly suited to treating occult, disseminated cancers. Astatine is not intrinsically tumour-specific; therefore, it requires an appropriate tumour-specific targeting vector, which can guide the radiation to the cancer cells. Consequently, an appropriate method is required for coupling the nuclide to the vector. To increase the availability of astatine-211 radiopharmaceuticals for targeted alpha therapy, their production should be automated. Here, we present a method that combines dry distillation of astatine-211 and a synthesis module for producing radiopharmaceuticals into a process platform. This platform will standardize production of astatinated radiopharmaceuticals, and hence, it will facilitate large clinical studies focused on this promising, but chemically challenging, alpha-emitting radionuclide. In this work, we describe the process platform, and we demonstrate the production of both astaine-211, for preclinical use, and astatine-211 labelled antibodies.

  16. CA 19-9 (Cancer Antigen 19-9)

    Science.gov (United States)

    ... as a tumor marker : To help differentiate between cancer of the pancreas and other conditions, such as pancreatitis To monitor ... levels of CA 19-9 are seen in cancer of the exocrine pancreas. This cancer arises in the tissues that produce ...

  17. Automated astatination of biomolecules - a stepping stone towards multicenter clinical trials

    DEFF Research Database (Denmark)

    Aneheim, Emma; Albertsson, Per; Bäck, Tom; Jensen, Holger; Palm, Stig; Lindegren, Sture

    2015-01-01

    vector, which can guide the radiation to the cancer cells. Consequently, an appropriate method is required for coupling the nuclide to the vector. To increase the availability of astatine-211 radiopharmaceuticals for targeted alpha therapy, their production should be automated. Here, we present a method......To facilitate multicentre clinical studies on targeted alpha therapy, it is necessary to develop an automated, on-site procedure for conjugating rare, short-lived, alpha-emitting radionuclides to biomolecules. Astatine-211 is one of the few alpha-emitting nuclides with appropriate chemical and...... challenging, alpha-emitting radionuclide. In this work, we describe the process platform, and we demonstrate the production of both astaine-211, for preclinical use, and astatine-211 labelled antibodies....

  18. Study of Astatine (III) reactions with O, S and N ligands in solution

    International Nuclear Information System (INIS)

    Full text of publication follows. Astatine (At, Z=85: [Xe]4f145d106s26p5) belongs to the halogen group and is located below iodine in the periodic table. One of its isotopes (211At) appears promising as a therapeutic agent in nuclear medicine (Ref.1) owing to the energy of the alpha particles emitted during the disintegration of its nucleus and its short physical half-life (7.2 h). Since there are no stable isotopes of astatine, the chemistry of this element remains poorly understood. Generally, At is supposed to behave as a halogen (Ref.2) but it has been shown recently in our group that astatine presents a metallic behaviour in aqueous solution: it notably exists as At+ and AtO+ species under the oxidation states +I and +III (Ref.3). At the present time, the number of studies dealing with the complexation properties of the cationic forms of astatine remains limited (Ref.4), owing to its low availability. In this work, we have investigated the reactions of AtO+ species with different hetero-atomic (N, S, O) model ligands. A combined approach based on experimental and theoretical studies has been used (Ref.5). On account of the difficulties of experimental investigations of astatine species, the reactivity of AtO+ was explored using a competition method founded on astatine distributions between two distinct phases. Furthermore, for each AtO+/ ligand complex, the nature of the species formed and the associated thermodynamic constants were determined by computational modeling (DFT calculations). In this framework, an original computational methodology was developed to take into account the specificities of astatine, notably the associated relativistic effects. The computed equilibrium constants have been confronted with the experimental results. This comparison demonstrates an outstanding coherence between experience and theory. Furthermore, the analysis of the results shows a key role of solvent effects on astatine chemistry. Lastly, a specific reactivity for the

  19. 46 CFR 199.60 - Communications.

    Science.gov (United States)

    2010-10-01

    ... of 47 CFR part 80. (b) Emergency position indicating radiobeacons (EPIRB) and search and rescue... 46 Shipping 7 2010-10-01 2010-10-01 false Communications. 199.60 Section 199.60 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Requirements for All Vessels § 199.60 Communications. (a)...

  20. 46 CFR 199.273 - Immersion suits.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Immersion suits. 199.273 Section 199.273 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.273 Immersion suits. (a) Each cargo vessel must carry an immersion suit approved under approval series 160.171 of...

  1. Some aspects of the organic, biological and inorganic chemistry of astatine

    International Nuclear Information System (INIS)

    Astatine has no stable isotopes and the radioactive isotopes with half-lives sufficiently long for chemical experiments (209At, 210At, 211At) must be produced artificially with a cyclotron or with a high energy accelerator by spallation of Th. This thesis deals with the synthesis and chemistry of At-compounds and the determination of some of their properties. (C.F.)

  2. Astatine-211 Pathway from Radiochemistry to Clinical Investigation

    International Nuclear Information System (INIS)

    Particularly in clinical settings where tumour burden is low and cancers are located in close proximity to essential normal tissue structures, α-particle emitting radionuclides can offer significant advantages for targeted radionuclide therapy. One of the first alpha emitters to be evaluated for this purpose is the 7.2-h half-life radiohalogen Astatine-211 (211At). From a commercialization-potential perspective 211At, is less appealing than the longer half-life alpha particle emitters Radium-223, Actinium-225 and Thorium-227, which have become the focus of many laboratories. However, if methods for providing a better supply of 211At could be developed, this alpha emitter would be the radionuclide of choice for many potential therapeutic applications. With regard to the production of 211At, this can be readily be accomplished by bombarding natural bismuth targets with 28−29.5 MeV alpha particles via the 209Bi(α,2n)211At reaction. The goal is to utilize an alpha particle beam energy that provides the required balance for maximizing 211At production while minimizing creation of 210At, which is problematic because of its 138.4-day half life alpha-particle emitting daughter, 210Po. For most intended clinical applications, alpha particle beam energy of about 29 MeV offers the best compromise between maximizing yield and providing 211At with sufficient radionuclidic purity for clinical use. Clinically relevant levels of 211At have been produced at several institutions using both internal and external cyclotron targets

  3. An attempt to explore the production routes of Astatine radionuclides: Theoretical approach

    OpenAIRE

    Maiti, Moumita; Lahiri, Susanta

    2008-01-01

    In order to fulfil the recent thrust of Astatine radionuclides in the field of nuclear medicine various production routes have been explored in the present work. The possible production routes of $^{209-211}$At comprise both light and heavy ion induced reactions at the bombarding energy range starting from threshold to maximum 100 MeV energy. For this purpose, we have used the nuclear reaction model codes TALYS, ALICE91 and PACE-II. Excitation functions of those radionuclides, produced throug...

  4. Direct astatination of a tumour-binding protein, human epidermal growth factor, using nido-carborane as a prosthetic group

    International Nuclear Information System (INIS)

    A method for direct astatine labeling of proteins has been investigated. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68 ± 9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. (author)

  5. Labelling prospects of astatine-211 with immunoglobulins (IgG): some general considerations

    International Nuclear Information System (INIS)

    The tumour therapeutic potential of the short lived alpha emitting radiohalogen 211At has been already been well recognised in the field of radioimmuno therapy. There is no evidence as such to show that astatine itself is a tumour seeking isotope. Therefore it has to be tagged to tumour seeking compound such as a drug or a protein preferably an antibody (IgG). In this communication, the labelling parameters which are required to be investigated for obtaining a stable product which could be useful as radioimmuno therapeutic agent, are described. (author). 6 refs

  6. Determination of the electron affinity of astatine and polonium by laser photodetachment

    CERN Multimedia

    We propose to conduct the first electron anity (EA) measurements of the two elements astatine (At) and polonium (Po). Collinear photodetachment spectroscopy will allow us to measure these quantities with an uncertainty limited only by the spectral linewidth of the laser. We plan to use negative ion beams of the two radioactive elements At and Po, which are only accessible on-line and at ISOLDE. The feasibility of our proposed method and the functionality of the experimental setup have been demonstrated at ISOLDE in o-line tests by the clear observation of the photodetachment threshold for stable iodine. This proposal is based on our Letter of Intent I-148 [1].

  7. Extraction of 211At-astatine from hydrochloric acid solutions by means of TOPO, TBP, and triphenylphosphine

    International Nuclear Information System (INIS)

    The extraction behaviour of astatine was studied under defined conditions from hydrochloride acid solutions (cHCl>0.1 M or 1 and 2M). Therefore other effects like adsorption, reduction or hydrolysis can be excluded. The present work describes the extraction with tri- n- octylphosphinoxide (TOPO), tri- n-butylphosphate (TBP) and tri-phenylphosphine in chloroform. (orig.)

  8. No-carrier-added astatination of N-succinimidyl-3-(tri-n-butylstannyl) benzoate (ATE) via electrophilic destannylation

    International Nuclear Information System (INIS)

    The no-carrier-added synthesis of N-succinimidyl 3-[211At]astato-benzoate from N-succinimidyl 3-(tri-n-butylstannyl)benzoate (ATE) is described. The nature of the solvent in which the 211At was isolated from the target was an important factor influencing both the radiochemical yields and the nature of the incorporated astatine activity. (orig.)

  9. 32 CFR 199.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... nursing services, home health aide services, physical therapy services, occupational therapy services... the mental health record. However, nursing and staff notes, no matter how complete, are not a... CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.2 Definitions. (a)...

  10. 32 CFR 199.17 - TRICARE program.

    Science.gov (United States)

    2010-07-01

    ... Defense (Health Affairs). In TRICARE, all care may be subject to review for medical necessity and... with guidelines issued by the Assistant Secretary of Defense for Health Affairs, certain travel... CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.17 TRICARE program....

  11. Incest - Strl. §§ 197 - 199

    OpenAIRE

    Rushiti, Besart

    2014-01-01

    I denne avhandlingen drøftes formålet og begrunnelsen for straffebudene i strl. §§ 197 - 199. Samtidig drøftes spørsmålet i et politisk perspektiv om straffeloven har som oppgave å håndheve moralske standarder eller grenser for seksuelle forhold mellom voksne innbyggere.

  12. 211At-Rh(16-S4-diol) complex as a precursor for astatine radiopharmaceuticals

    International Nuclear Information System (INIS)

    211At is one of the most promising radionuclides in α-radioimmunotherapy (α-RIT). Unfortunately, biomolecules labeled by direct electrophilic astatination are unstable due to the rapid loss of 211At under both in vitro and in vivo conditions. The present paper describes the results of our studies on attaching At- to the rhodium(III) complex with thioether ligand: 1,5,9,13-etrathiacyclohexadecane-3,11-diol (16-S4-diol). Rh3+ was chosen as a moderately soft metal cation which should form very strong bonds with soft At- anions, but first of all because of the kinetic inertness of low spin rhodium(III) d6 complexes. The 16-S4-diol ligand was selected due to formation of stable complexes with Rh3+. The experiments related to optimization of the reaction conditions were performed with the 131I, basing on a chemical similarity of I- to At-. The experiments with 211At were then carried out under the conditions found optimal for I-. The preliminary results are promising, and indicate a possibility for astatination of biomolecules by using the 211At-Rh(16-S4-diol) complex

  13. 49 CFR 199.219 - Pre-duty use.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Pre-duty use. 199.219 Section 199.219 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... Prevention Program § 199.219 Pre-duty use. Each operator shall prohibit a covered employee from using...

  14. 49 CFR 199.217 - On-duty use.

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    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false On-duty use. 199.217 Section 199.217 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... Prevention Program § 199.217 On-duty use. Each operator shall prohibit a covered employee from using...

  15. 22 CFR 19.9 - Pension benefits for former spouses.

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    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Pension benefits for former spouses. 19.9 Section 19.9 Foreign Relations DEPARTMENT OF STATE PERSONNEL BENEFITS FOR SPOUSES AND FORMER SPOUSES OF PARTICIPANTS IN THE FOREIGN SERVICE RETIREMENT AND DISABILITY SYSTEM § 19.9 Pension benefits for former spouses....

  16. 46 CFR 108.199 - Arrangement of sleeping spaces.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Arrangement of sleeping spaces. 108.199 Section 108.199... AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.199 Arrangement of sleeping spaces. To the extent practicable, each occupation group must be berthed together in sleeping...

  17. 49 CFR 199.107 - Drug testing laboratory.

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    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Drug testing laboratory. 199.107 Section 199.107... § 199.107 Drug testing laboratory. (a) Each operator shall use for the drug testing required by this part only drug testing laboratories certified by the Department of Health and Human Services under...

  18. 46 CFR 199.217 - Muster list and emergency instructions.

    Science.gov (United States)

    2010-10-01

    ... § 199.217 Muster list and emergency instructions. (a) The format of each passenger vessel muster list required under § 199.80 must be approved by the OCMI. (b) The passenger vessel muster list or emergency... 46 Shipping 7 2010-10-01 2010-10-01 false Muster list and emergency instructions. 199.217...

  19. 49 CFR 199.221 - Use following an accident.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Use following an accident. 199.221 Section 199.221... Prevention Program § 199.221 Use following an accident. Each operator shall prohibit a covered employee who has actual knowledge of an accident in which his or her performance of covered functions has not...

  20. Initial experience of thallium-199 application for myocardium perfusion scintiscanning

    International Nuclear Information System (INIS)

    Pharmocokinetics of thallium-199 in organs and tissues of animals with acute coronary occlusion is studied. Defects of perfusion in the back wall of myocardium in rabbits and in the front-partition part in dogs are visualized in myocardium scintigraphy with thallium-199 in animals with occlusion of coronary atrery. Scintigrams with thallium-201 carried out in animals after coronary occlusion were identical to the results of investigations with thallium-199. It is shown that thallium-199 is not at disadvantage to thallium-201 in its pharmacokinetic properties and in quality of scintigraphic pictures obtained and it can be used to detect coronary ischemia. 1 ref

  1. 7 CFR 800.199 - Conflict-of-interest provisions.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Conflict-of-interest provisions. 800.199 Section 800... Delegations, Designations, Approvals, Contracts, and Conflicts of Interest § 800.199 Conflict-of-interest...) Prohibited conflicts of interest. Unless waived on a case-by-case basis by the Administrator under section...

  2. Unexpected Behavior of the Heaviest Halogen Astatine in the Nucleophilic Substitution of Aryliodonium Salts.

    Science.gov (United States)

    Guérard, François; Lee, Yong-Sok; Baidoo, Kwamena; Gestin, Jean-François; Brechbiel, Martin W

    2016-08-22

    Aryliodonium salts have become precursors of choice for the synthesis of (18) F-labeled tracers for nuclear imaging. However, little is known on the reactivity of these compounds with heavy halides, that is, radioiodide and astatide, at the radiotracer scale. In the first comparative study of radiohalogenation of aryliodonium salts with (125) I(-) and (211) At(-) , initial experiments on a model compound highlight the higher reactivity of astatide compared to iodide, which could not be anticipated from the trends previously observed within the halogen series. Kinetic studies indicate a significant difference in activation energy (Ea =23.5 and 17.1 kcal mol(-1) with (125) I(-) and (211) At(-) , respectively). Quantum chemical calculations suggest that astatination occurs via the monomeric form of an iodonium complex whereas iodination occurs via a heterodimeric iodonium intermediate. The good to excellent regioselectivity of halogenation and high yields achieved with diversely substituted aryliodonium salts indicate that this class of compounds is a promising alternative to the stannane chemistry currently used for heavy radiohalogen labeling of tracers in nuclear medicine. PMID:27305065

  3. Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

    CERN Document Server

    Andreyev, Andrei

    2013-01-01

    Part I: $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with astatine beams; Part II: Delineating the island of deformation in the light gold isotopes by means of laser spectroscopy

  4. ASTATINE-211 RADIOCHEMISTRY: THE DEVELOPMENT OF METHODOLOGIES FOR HIGH ACTIVITY LEVEL RADIOSYNTHESIS

    Energy Technology Data Exchange (ETDEWEB)

    MICHAEL R. ZALUTSKY

    2012-08-08

    Targeted radionuclide therapy is emerging as a viable approach for cancer treatment because of its potential for delivering curative doses of radiation to malignant cell populations while sparing normal tissues. Alpha particles such as those emitted by 211At are particularly attractive for this purpose because of their short path length in tissue and high energy, making them highly effective in killing cancer cells. The current impact of targeted radiotherapy in the clinical domain remains limited despite the fact that in many cases, potentially useful molecular targets and labeled compounds have already been identified. Unfortunately, putting these concepts into practice has been impeded by limitations in radiochemistry methodologies. A critical problem is that the synthesis of therapeutic radiopharmaceuticals provides additional challenges in comparison to diagnostic reagents because of the need to perform radio-synthesis at high levels of radioactivity. This is particularly important for {alpha}-particle emitters such as 211At because they deposit large amounts of energy in a highly focal manner. The overall objective of this project is to develop convenient and reproducible radiochemical methodologies for the radiohalogenation of molecules with the {alpha}-particle emitter 211At at the radioactivity levels needed for clinical studies. Our goal is to address two problems in astatine radiochemistry: First, a well known characteristic of 211At chemistry is that yields for electrophilic astatination reactions decline as the time interval after radionuclide isolation from the cyclotron target increases. This is a critical problem that must be addressed if cyclotrons are to be able to efficiently supply 211At to remote users. And second, when the preparation of high levels of 211At-labeled compounds is attempted, the radiochemical yields can be considerably lower than those encountered at tracer dose. For these reasons, clinical evaluation of promising 211At

  5. 12 CFR 19.9 - Ex parte communications.

    Science.gov (United States)

    2010-01-01

    ... PROCEDURE Uniform Rules of Practice and Procedure § 19.9 Ex parte communications. (a) Definition—(1) Ex... judge may not consult a person or party on any matter relevant to the merits of the adjudication,...

  6. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE, CERN

    CERN Document Server

    Rothe, Sebastian; Nörtershäuser, W

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at ISOLDE, CERN, by the addition of an all-solid state tuneable titanium: sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE, CERN, and at ISAC, TRIUMF, radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  7. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    Energy Technology Data Exchange (ETDEWEB)

    Rothe, Sebastian

    2012-09-24

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  8. An all-solid state laser system for the laser ion sources RILIS and in-source laser spectroscopy of astatine at ISOLDE/CERN

    International Nuclear Information System (INIS)

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spectroscopy was performed at ISOLDE/CERN and at ISAC/TRIUMF radioactive ion beam facilities to develop an efficient and selective three-colour ionization scheme for the purely radioactive element astatine. A LabVIEW based monitoring, control and measurement system was conceived which enabled, in conjunction with Dual RILIS operation, the spectroscopy of high lying Rydberg states, from which the ionization potential of the astatine atom was determined for the first time experimentally.

  9. 32 CFR Appendix A to Part 199 - Acronyms

    Science.gov (United States)

    2010-07-01

    ... CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) Pt. 199, App. A Appendix A to Part... (Health Affairs) CCLR—Claims Collection Litigation Report CEOB—CHAMPUS Explanation of Benefits CFR—Code of Federal Regulations CHAMPUS—Civilian Health and Medical Program of the Uniformed Services...

  10. 32 CFR 199.4 - Basic program benefits.

    Science.gov (United States)

    2010-07-01

    ... connection with the furnishing of such nursing care; (C) Physical or occupational therapy or speech-language...) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.4 Basic program... as physicians, staff members of hospitals, or CHAMPUS health benefits advisors) have such...

  11. 27 CFR 26.199a - Action by revenue agent.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Action by revenue agent... Custody to Internal Revenue Bond § 26.199a Action by revenue agent. (a) Gauge. Puerto Rican spirits to be withdrawn for shipment to the United States as provided in this subpart shall be gauged by the revenue...

  12. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Science.gov (United States)

    2010-10-01

    ... from any projections of the vessel's structure or equipment. (4) The marine evacuation system's passage... 46 Shipping 7 2010-10-01 2010-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation...

  13. 26 CFR 1.199-6 - Agricultural and horticultural cooperatives.

    Science.gov (United States)

    2010-04-01

    ... application of this section: Example 1. (i) Cooperative X markets corn grown by its members within the United... receipts of $1,500,000, all derived from the sale of corn grown by its members within the United States. Cooperative X pays $370,000 for its members' corn and its W-2 wages (as defined in § 1.199-2(e)) for...

  14. 32 CFR 199.13 - TRICARE Dental Program.

    Science.gov (United States)

    2010-07-01

    ... irregularities to prevent ingress of oral fluids, food, and debris in order to prevent tooth decay. (c... Secretary of Defense (Health Affairs) (ASD(HA)). The Secretary of Defense, by 32 CFR part 367, delegated.... Implementing authority is contained in 32 CFR part 367. For additional implementing authority see § 199.1....

  15. 14 CFR 121.199 - Nontransport category airplanes: Takeoff limitations.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Nontransport category airplanes: Takeoff... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Airplane Performance Operating Limitations § 121.199 Nontransport category airplanes: Takeoff limitations. (a) No person operating...

  16. 27 CFR 26.199d - Customs inspection and release.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Customs inspection and... Shipment of Bulk Distilled Spirits From Puerto Rico, Without Payment of Tax, for Transfer From Customs Custody to Internal Revenue Bond § 26.199d Customs inspection and release. On receipt of a...

  17. 32 CFR 199.7 - Claims submission, review, and payment.

    Science.gov (United States)

    2010-07-01

    ... Note: For Federal Register citations affecting § 199.7, see the List of CFR Sections Affected, which... Nonavailability Statement. Appropriate policy guidance may be issued as necessary to prescribe the conditions for... information provided: (A) Name of drug. Note: When the physician or pharmacist so requests, the name of...

  18. 32 CFR 199.15 - Quality and utilization review peer review organization program.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Quality and utilization review peer review organization program. 199.15 Section 199.15 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.15 Quality...

  19. 49 CFR 40.199 - What problems always cause a drug test to be cancelled?

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false What problems always cause a drug test to be cancelled? 40.199 Section 40.199 Transportation Office of the Secretary of Transportation PROCEDURES FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Problems in Drug Tests § 40.199 What problems...

  20. Measurement of Linear Stark Interference in 199Hg

    CERN Document Server

    Loftus, T H; Griffith, W C; Romalis, M V; Heckel, B R; Fortson, E N

    2010-01-01

    We present measurements of Stark interference in the 6$^1S_0$ $\\rightarrow$ 6$^3P_1$ transition in $^{199}$Hg, a process whereby a static electric field $E$ mixes magnetic dipole and electric quadrupole couplings into an electric dipole transition, leading to $E$-linear energy shifts similar to those produced by a permanent atomic electric dipole moment (EDM). The measured interference amplitude, $a_{SI}$ = $(a_{M1} + a_{E2})$ = (5.8 $\\pm$ 1.5)$\\times 10^{-9}$ (kV/cm)$^{-1}$, agrees with relativistic, many-body predictions and confirms that earlier central-field estimates are a factor of 10 too large. More importantly, this study validates the capability of the $^{199}$Hg EDM search apparatus at the to resolve non-trivial, controlled, and sub-nHz Larmor frequency shifts with EDM-like characteristics.

  1. Measurement of linear stark interference in 199Hg.

    Science.gov (United States)

    Loftus, T H; Swallows, M D; Griffith, W C; Romalis, M V; Heckel, B R; Fortson, E N

    2011-06-24

    We present measurements of Stark interference in the (61)S(0)→6(3)P(1) transition in (199)Hg, a process whereby a static electric field E mixes magnetic dipole and electric quadrupole couplings into an electric dipole transition, leading to E-linear energy shifts similar to those produced by a permanent atomic electric dipole moment (EDM). The measured interference amplitude, a(SI) = (a(M1) + a(E2)) = (5.8 ± 1.5) × 10(-9) (kV / cm)(-1), agrees with relativistic, many-body predictions and confirms that earlier central-field estimates are a factor of 10 too large. More importantly, this study validates the capability of the (199)Hg EDM search apparatus to resolve nontrivial, controlled, and sub-nHz Larmor frequency shifts with EDM-like characteristics. PMID:21770639

  2. Measurement of Linear Stark Interference in Hg199

    Science.gov (United States)

    Loftus, T. H.; Swallows, M. D.; Griffith, W. C.; Romalis, M. V.; Heckel, B. R.; Fortson, E. N.

    2011-06-01

    We present measurements of Stark interference in the 6S01→6P13 transition in Hg199, a process whereby a static electric field E mixes magnetic dipole and electric quadrupole couplings into an electric dipole transition, leading to E-linear energy shifts similar to those produced by a permanent atomic electric dipole moment (EDM). The measured interference amplitude, aSI=(aM1+aE2)=(5.8±1.5)×10-9(kV/cm)-1, agrees with relativistic, many-body predictions and confirms that earlier central-field estimates are a factor of 10 too large. More importantly, this study validates the capability of the Hg199 EDM search apparatus to resolve nontrivial, controlled, and sub-nHz Larmor frequency shifts with EDM-like characteristics.

  3. Synthesis of MOF-199 and application to CO2 adsorption

    International Nuclear Information System (INIS)

    In this study a metal-organic framework (MOF-199) has been synthesized by solvent-thermal method. The conditions of preparation and activation processes have been investigated. The obtained material was characterized by methods of x-ray diffraction (XRD), infrared (IR) spectroscopy, thermogravimatric analysis (TGA) and scanning electron microscopy (SEM). The CO2 adsorption measurements were carried out on a high pressure volumetric analyzer (Micromeritics HPVA − 100). According to experimental results, Cu(NO3)2·3H2O has been shown to be the best copper(II) precursor for the synthesis of MOF-199 and N,N-dimethylformamide (DMF):C2H5OH:H2O with the ratio of 1:1:1 has been chosen as the most suitable solvent. The appropriate activation condition has been determined as follows: activate at 200 °C for 5 h and use CH3OH as the solvent to remove DMF. At the optimal conditions, an octahedral shape and three-dimensional (3D) structure of crystallite of MOF-199 was obtained. The synthesized MOF-199 expressed a high value of specific surface area (1448 m2 g−1 by Brunauer–Emmet–Teller (BET) method and 2028 m2 g−1 by Langmuir method) with Ta porous size of crystal of 11.8 Å and specific volume of 0.693 cm3 g−1; it was still stable up to 332 °C and its CO2 storage capacity reached to 206.59 cm3 (STP) g−1 at 25.76 bar. (paper)

  4. Elevated serum CA 19-9 at screening tests: underlying conditions and role of abdominopelvic CT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ji Yang [University of Louisville, Department of Radiology and Molecular Imaging Research Center, Louisville, KY (United States); Seoul National University Hospital, Department of Radiology, Healthcare System Gangnam Center, Seoul (Korea, Republic of); Kim, Se Hyung [Seoul National University Hospital, Department of Radiology and the Institute of Radiation Medicine, Seoul (Korea, Republic of); Kim, Soo Young [Seoul National University Hospital, Department of Radiology, Healthcare System Gangnam Center, Seoul (Korea, Republic of); Bundang CHA Hospital, Department of Radiology, Bundang (Korea, Republic of)

    2014-10-15

    To investigate underlying conditions of patients with elevated CA 19-9 at screening tests and to evaluate diagnostic performance of abdominopelvic CT. One hundred and thirteen patients with elevated CA 19-9 (>37 U/ml) who underwent abdominopelvic CT in a screening program were selected. Underlying conditions were determined by reviewing all available data and follow-up records. Patients were categorized into malignancy, benign, and normal/non-related disease groups. Their mean CA 19-9 and percent of patients with CA 19-9 ≥ 100 U/ml were compared. Diagnostic sensitivity of CT for detecting underlying conditions of elevated CA 19-9 was analysed. Seventeen patients (17/113, 15 %) had 17 elevated CA 19-9-related malignancies, and 55 patients (55/113, 48.7 %) had 70 benign diseases. Mean CA 19-9 and percent of patients with CA 19-9 ≥ 100 U/ml in the malignancy group were significantly higher than in the two other groups. CT detected all except one malignant lesion with a detection sensitivity of 94.1 % (16/17). Of 70 CA 19-9-related benign diseases, CT detected 34 benign diseases (48.6 %) providing an alternative diagnosis for elevated CA 19-9. Abdominopelvic CT is not only useful in detecting malignancies, but can also diagnose alternative benign causes of elevated CA 19-9 in asymptomatic screening tests. (orig.)

  5. Gastric Cancer with a Very High Serum CA 19-9 Level

    Directory of Open Access Journals (Sweden)

    Kazuya Kato

    2011-04-01

    Full Text Available Carbohydrate antigen 19-9 (CA 19-9 is a sensitive marker for pancreatic and hepatobiliary malignancies. The highest frequency of elevated serum CA 19-9 levels is found among patients with pancreatic cancer. CA 19-9 has recently been demonstrated to be a marker of digestive tract malignancies. We report the case of a patient with a gastric cancer and a very high serum CA 19-9 level. During laparotomy, a large mass was found in the antrum. A distal gastrectomy with D2 dissection of the lymph nodes was performed. Histological examination, including immunohistochemistry, revealed an adenocarcinoma of the stomach producing CA 19-9. To the best of our knowledge, no patient with an extremely high serum CA 19-9 level resulting from a gastric adenocarcinoma has been reported previously.

  6. Gastric Cancer with a Very High Serum CA 19-9 Level.

    Science.gov (United States)

    Kato, Kazuya; Taniguchi, Masahiko; Kawakami, Takako; Nagase, Atsushi; Matsuda, Minoru; Onodea, Kazuhiko; Yamaguchi, Hidenori; Higuchi, Mineko; Furukawa, Hiroyuki

    2011-01-01

    Carbohydrate antigen 19-9 (CA 19-9) is a sensitive marker for pancreatic and hepatobiliary malignancies. The highest frequency of elevated serum CA 19-9 levels is found among patients with pancreatic cancer. CA 19-9 has recently been demonstrated to be a marker of digestive tract malignancies. We report the case of a patient with a gastric cancer and a very high serum CA 19-9 level. During laparotomy, a large mass was found in the antrum. A distal gastrectomy with D2 dissection of the lymph nodes was performed. Histological examination, including immunohistochemistry, revealed an adenocarcinoma of the stomach producing CA 19-9. To the best of our knowledge, no patient with an extremely high serum CA 19-9 level resulting from a gastric adenocarcinoma has been reported previously. PMID:21577374

  7. Stability and in vivo behavior of Rh[16aneS4-diol]211At complex: A potential precursor for astatine radiopharmaceuticals

    International Nuclear Information System (INIS)

    Introduction: The heavy halogen 211At is of great interest for targeted radiotherapy because it decays by the emission of short-range, high-energy α-particles. However, many astatine compounds that have been synthesized are unstable in vivo, providing motivation for seeking other 211At labeling strategies. One relatively unexplored approach is to utilize prosthetic groups based on astatinated rhodium (III) complex stabilized with a tetrathioether macrocyclic ligand – Rh[16aneS4-diol]211At. The purpose of the current study was to evaluate the in vitro and in vivo stability of this complex in comparison to its iodine analog – Rh[16aneS4-diol]131I. Methods: Rh[16aneS4-diol]211At and Rh[16aneS4-diol]131I complexes were synthesized and purified by HPLC. The stability of both complexes was evaluated in vitro by incubation in phosphate-buffered saline (PBS) and human serum at different temperatures. The in vivo behavior of the two radiohalogenated complexes was assessed by a paired-label biodistribution study in normal Balb/c mice. Results: Both complexes were synthesized in high yield and purity. Almost no degradation was observed for Rh[16aneS4-diol]131I in PBS over a 72 h incubation. The astatinated analog exhibited good stability in PBS over 14 h. A slow decline in the percentage of intact complex was observed for both tracers in human serum. In the biodistribution study, retention of 211At in most tissues was higher than that of 131I at all time points, especially in spleen and lungs. Renal clearance of Rh[16aneS4-diol]211At and Rh[16aneS4-diol]131I predominated, with 84.1 ± 2.3% and 94.6 ± 0.9% of injected dose excreted via the urine at 4 h. Conclusions: The Rh[16aneS4-diol]211At complex might be useful for constructing prosthetic groups for the astatination of biomolecules and further studies are planned to evaluate this possibility

  8. 26 CFR 1.199-5 - Application of section 199 to pass-thru entities for taxable years beginning after May 17, 2006...

    Science.gov (United States)

    2010-04-01

    ... Reconciliation Act of 2005. 1.199-5 Section 1.199-5 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE... after May 17, 2006, the enactment date of the Tax Increase Prevention and Reconciliation Act of 2005. (a... receivables or inventory items within the meaning of section 751(c) or (d), respectively, and the sale...

  9. Measurement of Linear Stark Interference in 199Hg

    OpenAIRE

    Loftus, T. H.; Swallows, M. D.; Griffith, W. C.; Romalis, M. V.; Heckel, B. R.; Fortson, E. N.

    2010-01-01

    We present measurements of Stark interference in the 6$^1S_0$ $\\rightarrow$ 6$^3P_1$ transition in $^{199}$Hg, a process whereby a static electric field $E$ mixes magnetic dipole and electric quadrupole couplings into an electric dipole transition, leading to $E$-linear energy shifts similar to those produced by a permanent atomic electric dipole moment (EDM). The measured interference amplitude, $a_{SI}$ = $(a_{M1} + a_{E2})$ = (5.8 $\\pm$ 1.5)$\\times 10^{-9}$ (kV/cm)$^{-1}$, agrees with rela...

  10. Separation of 199Au (NCA) from neutron irradiated platinum on alumina

    International Nuclear Information System (INIS)

    High specific activity 199Au is mainly produced via 198Pt (n, y ) 199Pt → β- → 199Au. Gold-199 was produced by neutron irradiation of platinum metal. Platinum sponge (7.2% 198Pt) 10 mg was irradiated in a thermal neutron flux of 5 x 1013 cm-2 s-1 for 12 h and left at least 10 h to allow the neutron capture product 199Pt (T1/2= 30 m) to decay to 199Au. 195mPt (T1/2 = 4.02 d) and 197Pt (T1/2 = 20 h) were found to be the principal other radionuclides. The platinum sponge targets were dissolved in aqua regia and the solution was evaporated. Distilled water was added and evaporated twice at least. Finally the residue was dissolved in distilled water. Carrier-free radiochemical separation of 199Au from neutron-irradiated platinum targets was studied using targets of 10 mg of natural platinum metal (7.27% 198Pt). The sorption behaviour (Kd values) of Pt in the form of chloroplatinate and 199Au (NCA) in the form of chloroaurate on Al2O3 was investigated at different molarities of hydrochloric acid. The separation of 199Au (NCA) from platinum matrix activity on Al2O3 at molarity of 0.01 M HCl was carried out. A total yield of 85% 199Au was achieved, while contamination of Pt was less than 0.01%

  11. MiRNA-199a-5p influences pulmonary artery hypertension via downregulating Smad3.

    Science.gov (United States)

    Liu, Yuanhua; Liu, Guanghui; Zhang, Hui; Wang, Jing

    2016-05-13

    MicroRNAs (miRNAs) play important roles in pulmonary artery hypertension (PAH). Recently, it has been reported that miR-199a-5p participates in the progression of chronic obstructive pulmonary disease, ventricular hypertrophy and heart failure. However, the roles of miR-199a-5p in PAH are still unclear. In the present study, miR-199a-5p was investigated in PAH rat models and in human pulmonary artery smooth muscle cells (HPASMCs) and endothelial cells (HPAECs). The expression of miR-199a-5p was significantly increased following PAH induction, and anti-miR-199a-5p could increase the nitric oxide (NO) level and decrease the PAH-induced upregulation of pulmonary artery pressure and right ventricular hypertrophy. Moreover, in HPASMCs and HPAECs, miR-199a-5p overexpression could inhibit the level of NO and promote the concentration of Ca(2+), but anti-miR-199a-5p showed opposite results. Further analysis demonstrated that miR-199a-5p attenuated the expression of Smad3. Importantly, Smad3 was confirmed to be the target gene of miR-199a-5p using dual-luciferase reporter assay. Mechanism analyses revealed that the downregulation of NO and the upregulation of Ca(2+) caused by miR-199a-5p were all reversed by Smad3 overexpression in HPASMCs and HPAECs. Moreover, in PAH model, Smad3, p-Smad3 and Smad4 were all downregulated in lung tissues, and SIS3 (Smad3 inhibitor) could reverse the effects of anti-miR-199a-5p in PAH rats. Our date suggest that miR-199a-5p may function as a regulator of PAH by targeting Smad3, indicating a novel therapeutic strategy for patients with PAH. PMID:27038547

  12. 癌胚抗原199在卵巢畸胎瘤中的临床意义%Clinical significance of CA199 in ovarian teratoma

    Institute of Scientific and Technical Information of China (English)

    公莉; 王艳平; 汪秀芹; 王崇娟

    2012-01-01

    目的 探讨血清癌胚抗原199(CA199)在卵巢畸胎瘤诊断中的临床意义.方法 收集2005年6月至2010年6月日照市人民医院妇产科就诊的256例卵巢畸胎瘤患者为研究对象,比较CA199阳性率、CA199与卵巢畸胎瘤大小、单双侧及所合成分的关系.结果 4种肿瘤标记物中,CA199表达阳性率最高,且与畸胎瘤的大小、单双侧、所合成分等显著相关(P<0.05).结论 卵巢畸胎瘤的直径越大、为双侧、未成熟成分多时,阳性率明显升高,推测CA199在卵巢畸胎瘤的诊断、病情评估、提示预后方面具有一定的临床意义.%Objective To evaluate the clinical significance of CA199 in ovarian teratoma.Methods Two hundred and fifty-six patients with teratoma admitted in people' s hospital of rizhao between June 2005 and June 2010 were enrolled,the positive rate of CA199,relationships between CA199and ovarian teratoma size,single bilateral and compositions were compared.Results Among the four tumor markers,CA199 showed the highest positive rate.The level of CA199 had obvious correlation with the diameter and bilateral or unilateral and composition of the teratoma ( P < 0.05 ).Conclusions CA199may have significance in the diagnosis,evaluation,prognosis evaluation of ovarion teratoma.

  13. Improved limit on the permanent electric dipole moment of 199Hg

    CERN Document Server

    Griffith, W C; Loftus, T H; Romalis, M V; Heckel, B R; Fortson, E N

    2009-01-01

    We report the results of a new experimental search for a permanent electric dipole moment of 199Hg utilizing a stack of four vapor cells. We find d(199Hg) = (0.49 \\pm 1.29_stat \\pm 0.76_syst) x 10^{-29} e cm, and interpret this as a new upper bound, |d(199Hg)| < 3.1 x 10^{-29} e cm (95% C.L.). This result improves our previous 199Hg limit by a factor of 7, and can be used to set new constraints on CP violation in physics beyond the standard model.

  14. Well drilling summary report for well 199-N-106A

    International Nuclear Information System (INIS)

    Past liquid waste disposal practices within the 100-N Area have resulted in the contamination of the underlying sediments and groundwater. The release of large volumes of liquid effluent to the 1301-N and 1325-N Liquid Waste Disposal Facilities caused the transport of 90Sr and other contaminants to the groundwater. Further discussion of 100-N Area hydrogeology is provided in Hartman and Lindsey (1993). A pump-and-treat system combined with a vertical barrier is the preferred alternative for the N Springs Expedited Response Action. This document is a compilation of the data collected during the drilling of well 199-N-106A, an extraction well for the 100-N Pump-and-Treat Project

  15. 46 CFR 199.100 - Manning of survival craft and supervision.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Manning of survival craft and supervision. 199.100 Section 199.100 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LIFESAVING APPLIANCES....100 Manning of survival craft and supervision. (a) There must be a sufficient number of...

  16. Developement of an Immunoradiometric Assay for Tumour Marker CA19-9

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A two-site sandwich immunoradiometric assay for quantifying CA19-9 in serum is developed byusing two monoclonal antibodies. One of CA19-9 antibodies is labeled with 125I as a tracer and the othermonoclonal antibody is immobilized on plastic tubes as the solid phase. The sensitivity of the assay is 2

  17. 46 CFR 199.214 - Immersion suits and thermal protective aids.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must carry at least three immersion suits approved under approval series 160.171 for each lifeboat on...

  18. Final Report for research grant "Development of Methods for High Specific Activity Labeling of Biomolecules Using Astatine-211 in Different Oxidation States"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, D., Scott

    2011-12-14

    The overall objective of this research effort was to develop methods for labeling biomolecules with higher oxidation state species of At-211. This was to be done in an effort to develop reagents that had higher in vivo stability than the present carbon-bonded At-211-labeled compounds. We were unsuccessful in that effort, as none of the approaches studied provided reagents that were stable to in vivo deastatination. However, we gained a lot of information about At-211 in higher oxidation states. The studies proved to be very difficult as small changes in pH and other conditions appeared to change the nature of the species that obtained (by HPLC retention time analyses), with many of the species being unidentifiable. The fact that there are no stable isotopes of astatine, and the chemistry of the nearest halogen iodine is quite different, made it very difficult to interpret results of some experiments. With that said, we believe that a lot of valuable information was obtained from the studies. The research effort evaluated: (1) methods for chemical oxidation of At-211, (2) approaches to chelation of oxidized At-211, and (3) approaches to oxidation of astatophenyl compounds. A major hurdle that had to be surmounted to conduct the research was the development of HPLC conditions to separate and identify the various oxidized species formed. Attempts to develop conditions for separation of iodine and astatine species by normal and reversed-phase TLC and ITLC were not successful. However, we were successful in developing conditions (from a large number of attempts) to separate oxidized forms of iodine ([I-125]iodide, [I-125]iodate and [I-125]periodate) and astatine ([At-211]astatide, [At-211]astatate, [At-211]perastatate, and several unidentified At-211 species). Information on the basic oxidation and characterization of At-211 species is provided under Objective 1. Conditions were developed to obtain new At-211 labeling method where At-211 is chelated with the DOTA and

  19. Establishment of radioactive astatine and iodine uptake in cancer cell lines expressing the human sodium/iodide symporter

    International Nuclear Information System (INIS)

    The sodium/iodide symporter (NIS) has been recognized as an attractive target for radioiodine-mediated cancer gene therapy. In this study we investigated the role of human NIS for cellular uptake of the high LET α-emitter astatine-211 (211At) in comparison with radioiodine as a potential radionuclide for future applications. A mammalian NIS expression vector was constructed and used to generate six stable NIS-expressing cancer cell lines (three derived from thyroid carcinoma, two from colon carcinoma, one from glioblastoma). Compared with the respective control cell lines, steady state radionuclide uptake of NIS-expressing cell lines increased up to 350-fold for iodine-123 (123I), 340-fold for technetium-99m pertechnetate (99mTcO4-) and 60-fold for 211At. Cellular 211At accumulation was found to be dependent on extracellular Na+ ions and displayed a similar sensitivity towards sodium perchlorate inhibition as radioiodide and 99mTcO4- uptake. Heterologous competition with unlabelled NaI decreased NIS-mediated 211At uptake to levels of NIS-negative control cells. Following uptake both radioiodide and 211At were rapidly (apparent t1/2 3-15 min) released by the cells as determined by wash-out experiments. Data of scintigraphic tumour imaging in a xenograft nude mice model of transplanted NIS-modified thyroid cells indicated that radionuclide uptake in NIS-expressing tumours was up to 70 times (123I), 25 times (99mTcO4-) and 10 times (211At) higher than in control tumours or normal tissues except stomach (3-5 times) and thyroid gland (5-10 times). Thirty-four percent and 14% of the administered activity of 123I and 211At, respectively, was found in NIS tumours by region of interest analysis (n=2). Compared with cell culture experiments, the effective half-life in vivo was greatly prolonged (6.5 h for 123I, 5.2 h for 211At) and preliminary dosimetric calculations indicate high tumour absorbed doses (3.5 Gy/MBqtumour for 131I and 50.3 Gy/MBqtumour for 211At). In

  20. Intralobar pulmonary sequestration associated with marked elevation of serum carbohydrate antigen 19-9.

    Science.gov (United States)

    Ambiru, Satoshi; Nakamura, Shunta; Fukasawa, Motoji; Mishima, Osamu; Kuwahara, Takeichiro; Takeshi, Akihiko

    2009-12-01

    This report describes a 62-year-old man who experienced elevated serum carbohydrate antigen 19-9 (CA19-9) levels (>500 U/mL) for 4 years, and was finally diagnosed with right intralobar pulmonary sequestration. Surgery confirmed the presence an aberrant artery arising from the descending thoracic aorta and entering the right lower lobe basal segment. Immunohistochemistry demonstrated markedly positive staining of CA19-9 in the ciliated cylindrical epithelia, alveoli, and mucus in the cysts. After pulmonary resection, CA19-9 levels decreased to within a normal range. Therefore, the cause of the elevated serum CA19-9 levels in this case was almost certainly due to intralobar pulmonary sequestration. PMID:19932280

  1. Neutron cross sections for 93Nb(n, n')93mNb and 199Hg(n, n')199mHg reactions compiled in JENDL dosimetry file

    International Nuclear Information System (INIS)

    Evaluation procedure of neutron cross sections and covariance matrixes for 93Nb(n, n')93mNb and 199Hg(n, n')199mHg reactions is described in detail. The C/E value of 235U fission spectrum averaged cross section is 0.99 for 93Nb(n, n')93mNb reaction and 0.86 for 199Hg(n, n')199mHg reaction, respectively. (author)

  2. Comparative study of CA242 and CA19-9 for the diagnosis of pancreatic cancer.

    OpenAIRE

    Kawa, S; Tokoo, M.; Hasebe, O.; Hayashi, K.; Imai, H; Oguchi, H.; Kiyosawa, K; Furuta, S; Homma, T

    1994-01-01

    A comparative study of a new tumour marker, CA242, and CA19-9 was conducted with special reference to their diagnostic usefulness in pancreatic cancer. CA242 showed sensitivity similar to that of CA19-9 for overall cases and early cases (stage I tumour) of pancreatic cancer. For other malignancies, the positive rates of CA242 were lower than those of CA19-9 except for colorectal cancer. An important characteristics of CA242 was that it was only slightly and infrequently elevated in the sera o...

  3. Establishment of radioactive astatine and iodine uptake in cancer cell lines expressing the human sodium/iodide symporter

    Energy Technology Data Exchange (ETDEWEB)

    Petrich, T.; Helmeke, H.-J.; Meyer, G.J.; Knapp, W.H.; Poetter, E. [Department of Nuclear Medicine, Medizinische Hochschule Hannover (Germany)

    2002-07-01

    The sodium/iodide symporter (NIS) has been recognized as an attractive target for radioiodine-mediated cancer gene therapy. In this study we investigated the role of human NIS for cellular uptake of the high LET {alpha}-emitter astatine-211 ({sup 211}At) in comparison with radioiodine as a potential radionuclide for future applications. A mammalian NIS expression vector was constructed and used to generate six stable NIS-expressing cancer cell lines (three derived from thyroid carcinoma, two from colon carcinoma, one from glioblastoma). Compared with the respective control cell lines, steady state radionuclide uptake of NIS-expressing cell lines increased up to 350-fold for iodine-123 ({sup 123}I), 340-fold for technetium-99m pertechnetate ({sup 99m}TcO{sub 4}{sup -}) and 60-fold for {sup 211}At. Cellular {sup 211}At accumulation was found to be dependent on extracellular Na{sup +} ions and displayed a similar sensitivity towards sodium perchlorate inhibition as radioiodide and {sup 99m}TcO{sub 4}{sup -} uptake. Heterologous competition with unlabelled NaI decreased NIS-mediated {sup 211}At uptake to levels of NIS-negative control cells. Following uptake both radioiodide and {sup 211}At were rapidly (apparent t{sub 1/2} 3-15 min) released by the cells as determined by wash-out experiments. Data of scintigraphic tumour imaging in a xenograft nude mice model of transplanted NIS-modified thyroid cells indicated that radionuclide uptake in NIS-expressing tumours was up to 70 times ({sup 123}I), 25 times ({sup 99m}TcO{sub 4}{sup -}) and 10 times ({sup 211}At) higher than in control tumours or normal tissues except stomach (3-5 times) and thyroid gland (5-10 times). Thirty-four percent and 14% of the administered activity of {sup 123}I and {sup 211}At, respectively, was found in NIS tumours by region of interest analysis (n=2). Compared with cell culture experiments, the effective half-life in vivo was greatly prolonged (6.5 h for {sup 123}I, 5.2 h for {sup 211}At) and

  4. The Role of CA19-9 in Predicting Tumour Resectability in Carcinoma Head of Pancreas

    Science.gov (United States)

    Viswanathan, Subramanian; Antomy, Thomas Babu; Thirumuruganand, Sathyamoorthy; Kumaresan, Dhandapani Subramanian

    2016-01-01

    Introduction Carbohydrate antigen 19-9 (CA 19-9) is a tumour associated antigen. Blood levels may be elevated in benign as well as malignant conditions. Its sensitivity (70-90%) and specificity (68-91%) are inadequate for accurate diagnosis. It can be used to predict the extent of disease and outcome after resection. Aim The aim of the present study was to assess the role of CA 19-9 in predicting the resectability of the tumour in the head of pancreas. Materials and Methods This was a prospective study which included 30 patients and study period was from May 2012 to October 2014. Data collected from all patients with carcinoma of the head of pancreas on the basis of contrast enhanced computed tomography/Magnetic resonance cholangiopancreaticography. CA 19-9 levels were measured and recorded. Patients who were medically unfit for surgery or those who didn’t warrant palliative surgery were excluded from the study. During surgery the operative findings on operability were documented and tabulated against corresponding CA 19-9 levels. Results Of the 30 patients who were operated, 13(43.3%) patients had operable tumours and underwent Whipple’s procedure and 17(56.7%) underwent palliative bypass procedure. Of the 30, CA 19-9 levels were elevated in 9(30.0%) and were normal in 21(70.0%). Among 13(43.3%) who had operable tumours, CA 19-9 was elevated in 4(13.3) and was normal in 9(30.0%). Of the 17(56.7%) who had inoperable tumours CA 19-9 was elevated in 5(16.7%) and was normal in 12(40.0%). Among the 17 who had inoperable tumours, 8(47.1%) were diagnosed preoperatively and of them CA 19-9 levels were raised in 2(11.8%) and normal in 6(35.3%). In the group of 9(52.9%) who had inoperable tumours diagnosed intraoperatively, CA 19-9 was raised in 3(17.6%) of them and was normal in the remaining 6(35.3%) of them. Conclusion Based on the study findings, it can be stated that there is no significant correlation with resectability of pancreatic adenocarcinoma and CA 19-9 and

  5. Thallium-199 myocardial scintigraphy for assessing the efficacy of drug and surgical treatment of coronary disease

    International Nuclear Information System (INIS)

    Potentialities of thallium-199 myocardial scintigraphy in evaluation of the effects of surgical and drug treatment of myocardial perfusion in coronary patients are studied. It is shown that thallium-199 myocardial scintigraphy helps assess the effect of treatment on myocardial perfusion. Positive effect of treatment manifests by decrease of myocardial perfusion defects. The method permits imaging the coronary microcirculation over the course of treatment and predicting a further course of disease

  6. Comparative study of CA242 and CA19-9 in chronic pancreatitis.

    OpenAIRE

    Furuya, N; Kawa, S; Hasebe, O.; Tokoo, M.; Mukawa, K.; Maejima, S.; Oguchi, H.

    1996-01-01

    CA242 has been proved to be useful in the diagnosis of pancreatic cancer. The aim of the present study was to clarify the mechanisms contributing to the high specificity of CA242 as compared with CA19-9 resulting from scarce serum elevation of this antigen in patients with chronic pancreatitis by correlating serum levels and endoscopic retrograde choledocho-pancreatography (ERCP) findings and by immunohistochemical analysis. Serum CA19-9 levels were significantly elevated in patients with cal...

  7. Reduced Limit on the Permanent Electric Dipole Moment of $^{199}$Hg

    OpenAIRE

    Graner, B.; Chen, Y.; Lindahl, E. G.; Heckel, B. R.

    2016-01-01

    This paper describes the results of the most recent measurement of the permanent electric dipole moment (EDM) of neutral $^{199}$Hg atoms. Fused silica vapor cells containing enriched $^{199}$Hg are arranged in a stack in a common magnetic field. Optical pumping is used to spin-polarize the atoms orthogonal to the applied magnetic field, and the Faraday rotation of near-resonant light is observed to determine an electric-field-induced perturbation to the Larmor precession frequency. Our resul...

  8. Atomic mass determinations for 183W and 199Hg and the mercury problem

    International Nuclear Information System (INIS)

    Recent modifications to the 'Manitoba II' high resolution mass spectrometer are described. Mass differences among the members of the triplet 199 Hg -183W 16O- 12C 235 Cl 5 have been measured. These self-consistent mass differences give masses for 183W and 199Hg, as well as the mass difference across the W to Hg region of the mass table. These masses and the mass difference provide important constraints for the least squares atomic mass evaluation

  9. Abnormal Elevated CA 19-9 in the Dermoid Cyst: A Sign of the Ovarian Torsion?

    Directory of Open Access Journals (Sweden)

    Burcu Artunc Ulkumen

    2013-01-01

    Full Text Available Dermoid cyst is the most common germ cell tumor of the ovary containing various tissue elements. Ovarian torsion is a common complication of which ultrasonographic diagnosis is confusing. We report here a 14-year-old adolescent with painless torsion of the ovary including dermoid cyst and with abnormal elevated CA 19-9 serum levels. Elevated CA 19-9 level may be related to ovarian torsion and may predict the extent of tissue necrosis.

  10. Abnormal Elevated CA 19-9 in the Dermoid Cyst: A Sign of the Ovarian Torsion?

    OpenAIRE

    Burcu Artunc Ulkumen; Asli Goker; Halil Gursoy Pala; Sercin Ordu

    2013-01-01

    Dermoid cyst is the most common germ cell tumor of the ovary containing various tissue elements. Ovarian torsion is a common complication of which ultrasonographic diagnosis is confusing. We report here a 14-year-old adolescent with painless torsion of the ovary including dermoid cyst and with abnormal elevated CA 19-9 serum levels. Elevated CA 19-9 level may be related to ovarian torsion and may predict the extent of tissue necrosis.

  11. Comparison experiments of neon and helium buffer gases cooling in trapped 199Hg+ ions linear trap

    International Nuclear Information System (INIS)

    The influences of different buffer gas, neon and helium, on 199Hg+ clock transition are compared in trapped 199Hg+linear trap. By the technique of time domain's Ramsey separated oscillatory fields, the buffer gas pressure frequency shifts of 199Hg+ clock transition are measured to be (df/dPNe)(1/f) = 1.8 × 10−8 Torr−1 for neon and (df/dPHe)(1/f) = 9.1×10−8 Torr−1 for helium. Meanwhile, the line-width of 199Hg+ clock transition spectrum with the buffer gas neon is narrower than that with helium at the same pressure. These experimental results show that neon is a more suitable buffer gas than helium in 199Hg+ ions microwave frequency standards because of the 199Hg+ clock transition is less sensitive to neon variations and the better cooling effect of neon. The optimum operating pressure for neon is found to be about 1.0×10−5 Torr in our linear ion trap system. (atomic and molecular physics)

  12. Pancreatic Mucinous Cyst Adenocarcinoma Producing CA 19-9. A Case Report

    Directory of Open Access Journals (Sweden)

    Sumiya Ishigami

    2007-03-01

    Full Text Available Context We herein present a rare case of a mucinous cystic neoplasm of the pancreas producing CA 19-9 and the clinical implications are discussed. Case report A 35-year-old woman with no history of abdominal surgery presented at Saisei Kai Sendai Hospital with an upper abdominal distention. Abdominal CT showed a large lobulated cystic tumor at the pancreatic tail. No distant metastases were identified. The preoperative serum CA 19-9 level was 6,200 U/mL (reference range: 0-37 U/mL. A mucinous cystic neoplasm of the pancreas was diagnosed and elective surgery was performed. On laparotomy, a round tumor 15 cm in diameter was encountered in the upper left abdomen. No invasion of neighboring organs or the portal vein was apparent. The entire tumor was curatively resected with a distal pancreatectomy. The final histopathological analysis revealed mucinous cystadenocarcinoma with no invasive component. Immunohistochemical staining disclosed CA 19-9 expression within the tumor cells. The CA 19-9 level normalized rapidly postoperatively and, although a minor pancreatic fistula occurred, this was resolved conservatively. She was discharged on the 45th postoperative day with no sign of tumor relapse; her CA 19-9 level was within the normal range 20 months postoperatively. Conclusion We present this rare case of a mucinous cystic neoplasm producing CA 19-9 and discuss the relevant literature. The CA 19-9 production in this tumor does not appear to be directly correlated to aggressive clinical behavior.

  13. CA 19-9 is an index of the response to neoadjunctive chemoradiation therpay in pancreatic cancer

    International Nuclear Information System (INIS)

    Purpose: This study examines the changes of serum levels of CA 19-9 in patients with pancreatic cancer following neoadjuvant irradiation and chemotherapy to define the potential role of this tumor marker in preoperative management of these patients. Materials and Methods: Serum CA 19-9 levels were measured in 42 patients before receiving external beam irradiation with concurrent 5-fluorouracil in preparation for laparotomy and Whipple procedure or intraoperative irradiation (IORT). The CA 19-9 levels were determined again after irradiation, and changes were correlated with findings of restaging CT scan and laparatomy. Results: Following preoperative irradiation, 10 patients (24%) experienced an increase in CA 19-9 levels whereas 29 patients (69%) showed a decrease in CA 19-9. There was no change in the CA 19-9 levels of three patients (7%) after treatment. Of the 10 patients with increased CA 19-9 levels after irradiation, 9 patients (90%) had developed distant metastases or local tumor progression as determined by restaging CT scan or at laparotomy. In contrast, only 6 of 29 patients (21%) with declining CA 19-9 levels after irradiation demonstrated metastases or local tumor progression on restaging CT scan or at laparotomy. The correlation of CA 19-9 increase or decrease with disease progression or control, respectively, was statistically significant (p=0.009). Conclusions: Serum CA 19-9 levels may rise or fall during neoadjuvant therapy. A rising CA 19-9 reliably indicates cancer progression while a falling CA 19-9 connotes disease control in the majority of patients. In developing strategies for application of neoadjuvant therapy for pancreatic cancer, monitoring of CA 19-9 appears most useful for the identification of patients who manifest progressive tumor growth and metastasis in spite of this treatment

  14. Metastatic prostate cancer with elevated serum levels of CEA and CA19-9

    Directory of Open Access Journals (Sweden)

    Guang-Dar Juang

    2014-03-01

    Full Text Available Prostate-specific antigen (PSA is well known as a specific tumor marker for prostate cancer, but carcinoembryonic antigen (CEA- and carbohydrate antigen 19-9 (CA19-9-elevating adenocarcinomas originating in the prostate gland are rare. We report a case of metastatic adenocarcinoma of the prostate gland with a high serum level of CEA and CA19-9 in a 78-year-old man in whom prostate cancer (T3N1M1 had been diagnosed 2 years ago and who was treated with androgen deprivation therapy. He visited the emergency department because of a loss of appetite and abdominal pain. The serum CEA and CA19-9 levels were increased to 218.9 ng/mL (normal, <5 ng/mL and 212 ng/mL (normal, <27 ng/mL, respectively. The serum PSA level was slightly elevated (4.41 ng/mL. Computed tomography demonstrated multiple liver metastases, para-aortic lymph node enlargement, and lung metastases. A liver biopsy was performed and the specimen showed high-grade adenocarcinoma with focal positive staining for PSA. Despite chemotherapy with docetaxel, the patient died 3 months after treatment. Based on this case and a review of the literature, an aggressive variant of prostatic carcinoma with a high serum level of CEA and CA19-9 and a low PSA level was shown to progress rapidly with a poor prognosis.

  15. Production of radio-gold {sup 199}Au for diagnostic and therapeutic applications

    Energy Technology Data Exchange (ETDEWEB)

    Khandaker, Mayeen Uddin, E-mail: mu-khandaker@um.edu.my; Kassim, Hasan Abu, E-mail: hasanak@um.edu.my [Department of Physics, University of Malaya, 50603 Kuala Lumpur (Malaysia); Haba, Hiromitsu, E-mail: haba@riken.jp [Nishina Center for Accelerator-Based Science, RIKEN, Wako, Saitama 351-0198 (Japan)

    2016-01-22

    Production cross-sections of the {sup nat}Pt(d,x){sup 199}Au reactions have been measured from a 24-MeV deuteron energy down to the threshold by using a stacked-foil activation technique combined with HPGe γ-ray spectrometry. Only a partial agreement is obtained with the existing literature data. Theoretical data extracted from the TENDL-2013 library shows large discrepancy with the measured ones. Physical thick target yield for the {sup 199}Au radionuclide was deduced using the measured cross-sections, and found a general agreement with the directly measured yield available in the literature. This study reveals that a low deuteron energy (<15 MeV) cyclotron and an enriched {sup 198}Pt (100%) target could be used to obtain {sup 199}Au in no carrier added form.

  16. 199Hg and 63,65Cu NMR studies of mercury based high-Tc superconductors

    International Nuclear Information System (INIS)

    Hg-oxide ceramic high temperature superconductors were studied with 199Hg- and 63,65Cu- NMR spectroscopy. Room temperature spectra, spin-spin and spin-lattice relaxation times of samples with different superconducting transition temperatures are presented. A spin-lattice relaxation time of T1 = 35 msec and a spin-spin relaxation time of T2 = 1.6 msec is found for the 199 Hg-NMR. All samples exhibit similar characteristic powder spectra caused by an axially symmetric 199Hg spin interaction. The isotropic value and the anisotropy of the tensor relative to solid HgCl2 as a standard substance is estimated. Furthermore results of 63,65Cu NMR at a temperature of 4.2 K are presented. Also this signal shows a powder line shape (for I = 3/2)

  17. Microsolvation of methylmercury: structures, energies, bonding and NMR constants ((199)Hg, (13)C and (17)O).

    Science.gov (United States)

    Flórez, Edison; Maldonado, Alejandro F; Aucar, Gustavo A; David, Jorge; Restrepo, Albeiro

    2016-01-21

    Hartree-Fock (HF) and second order perturbation theory (MP2) calculations within the scalar and full relativistic frames were carried out in order to determine the equilibrium geometries and interaction energies between cationic methylmercury (CH3Hg(+)) and up to three water molecules. A total of nine structures were obtained. Bonding properties were analyzed using the Quantum Theory of Atoms In Molecules (QTAIM). The analyses of the topology of electron densities reveal that all structures exhibit a partially covalent HgO interaction between methylmercury and one water molecule. Consideration of additional water molecules suggests that they solvate the (CH3HgOH2)(+) unit. Nuclear magnetic shielding constants σ((199)Hg), σ((13)C) and σ((17)O), as well as indirect spin-spin coupling constants J((199)Hg-(13)C), J((199)Hg-(17)O) and J((13)C-(17)O), were calculated for each one of the geometries. Thermodynamic stability and the values of NMR constants correlate with the ability of the system to directly coordinate oxygen atoms of water molecules to the mercury atom in methylmercury and with the formation of hydrogen bonds among solvating water molecules. Relativistic effects account for 11% on σ((13)C) and 14% on σ((17)O), which is due to the presence of Hg (heavy atom on light atom, HALA effect), while the relativistic effects on σ((199)Hg) are close to 50% (heavy atom on heavy atom itself, HAHA effect). J-coupling constants are highly influenced by relativity when mercury is involved as in J((199)Hg-(13)C) and J((199)Hg-(17)O). On the other hand, our results show that the values of NMR constants for carbon and oxygen, atoms which are connected through mercury (C-HgO), are highly correlated and are greatly influenced by the presence of water molecules. Water molecules introduce additional electronic effects to the relativistic effects due to the mercury atom. PMID:26670708

  18. Updated measurement of the permanent electric dipole moment (EDM) of 199 Hg

    Science.gov (United States)

    Graner, Brent; Chen, Yi; Lindahl, Eric; Heckel, Blayne

    2016-05-01

    A permanent electric dipole moment (EDM) in an atom or particle would prove that time reversal symmetry is broken. In addition, an atomic EDM may provide evidence of new physics or CP symmetry violation in the strong sector. We have recently completed an improved measurement of the EDM of 199 Hg utilizing a set of vapor cells containing isotopically-enriched 199 Hg optically pumped and probed with UV laser light. I will discuss the most recent iteration of the experiment, and present unblinded results. This work was supported by NSF Grant 1306743 and DOE Award No. DE-FG02-97ER41020.

  19. The clinical significance of CA199 in mature ovarian teratoma%糖抗原199在卵巢成熟畸胎瘤诊断中的临床意义

    Institute of Scientific and Technical Information of China (English)

    王伟萍; 向阳; 沈铿; 冯凤芝; 万希润; 郎景和

    2011-01-01

    目的 探讨糖抗原199(CA199)在卵巢成熟畸胎瘤诊断中的临床意义.方法 选取2004年1月至2008年10月在北京协和医院妇产科进行手术并且术前进行包括CA199在内的4种肿瘤标志物检查的卵巢畸胎瘤患者263例进行分析.结果 在4种肿瘤标志物中,CA199的阳性率最高,为38.4%.4例有畸胎瘤剔除史患者的CA199水平均高于正常.畸胎瘤的最大径线平均大小为(7.07 ±3.22)cm.CA199、CA125升高组肿瘤大于正常组(P<0.001).当肿瘤为多房表现时,血清CA199、CA125水平较单房者明显升高(P<0.05);当肿瘤发生于双侧卵巢时,CA199显著升高(P<0.05).结论 CA199在卵巢成熟畸胎瘤的诊断与鉴别诊断中具有一定的意义.当血清CA199水平升高时,无论对侧卵巢外观是否正常,手术时均应仔细检查对侧卵巢.CA199是否可作为临床检测卵巢畸胎瘤复发的指标有待于进一步的研究证实.%Objective To evaluate the clinical significance of CA199 in mature ovarian teratoma. Methods We retrospectively studied 263 patients operated at Peking Uinion Medical College Hospital between January 2004 and October 2008, whose tumor markers including CA199 was studied before the operation. Results Among the four tumor markers,CA199 showed the highest positive rate of 38. 4%. CA199 of 4 cases with history of teratoma were all elevated. The mean tumor diameter was (7.07 ±3. 22)cm. The mean tumor size of patients with elevated CA 199、CA125 was greater than those with normal CA199 、CA125 level (P <0. 001 ). Serum CA199, CA125 levels were significantly higher in those with multi-room teratoma than with single-room teratoma ( P < 0. 05 ). Patients with bilateral teratomas showed a significantly higher rate of CA199 than those with unilateral ones( P < 0. 05 ). Conclusion CA199 may have significance in the diagnosis of mature ovarian teratoma. Whether CA199 be used as a marker for detecting mature ovarian teratoma recurrence needs

  20. Up-regulated miR-199a-5p in gastric cancer functions as an oncogene and targets klotho

    International Nuclear Information System (INIS)

    Recent studies have shown that miR-199a-5p plays opposite roles in cancer initiation and progression of different cancer types, acting as oncogene for some cancer types but as tumor suppressor gene for others. However, the role and molecular mechanism of miR-199a-5p in gastric cancer are largely unknown. In this study, miR-199a-5p expression level in gastric cancer was first analyzed by qPCRand then validated in 103 gastric cancer patients by in situ hybridization (ISH). Gastric cancer cell lines were transfected with miR-199a-5p inhibitor and mimic, and underwent in vitro transwell assays. Target genes (klotho) were identified using Luciferase reporter assay. Immunohistochemical staining was also used to investigate on how miR-199a-5p regulates the tumour-suppressive effects of klotho in gastric cancer. In our present study, we found that miR-199a-5p level was significantly increased in gastric cancer tissues compared to paired normal tissues. We observed that miR-199a-5p could promote migration and invasion of gastric cancer cells. In situ hybridization of miR-199a-5p also confirmed that higher miR-199a-5p expression level was associated with increased likelihood of lymph node metastasis and later TNM stage. Luciferase reporter assay and immunohistochemistry revealed that klotho might be the downstream target of miR-199a-5p. Our present study suggests that miR-199a-5p acts as an oncogene in gastric cancer and functions by targeting klotho

  1. Propofol inhibits the adhesion of hepatocellular carcinoma cells by upregulating microRNA-199a and downregulating MMP-9 expression

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    BACKGROUND: Propofol  is  one  of  the  extensively  and commonly  used  intravenous  anesthetics  and  has  the  ability to  influence  the  proliferation,  motility,  and  invasiveness  of many  cancer  cells.  In  this  study,  the  effects  of  propofol  on hepatocellular carcinoma cells invasion ability were examined. METHODS: We assessed the invasion ability of HepG2 cells in vitro  by  determining  enzyme  activity  and  protein  expression of  MMP-9  using  gelatin  zymography  assay  and  Western  blot. The real-time PCR was used to evaluate the effect of propofol on  microRNA-199a  (miR-199a)  expression,  and  miR-199a-2 precursor  to  evaluate  whether  over-expression  of  miR-199a can affect MMP-9 expression. Finally, the effect of miR-199a on propofol-induced anti-tumor activity using anti-miR-199a was assessed. RESULTS: Propofol  significantly  elevated  the  expression of  miR-199a  and  inhibited  the  invasiveness  of  HepG2  cells. Propofol  also  efficiently  decreased  enzyme  activity  and protein  expression  of  MMP-9.  Moreover,  the  over-expression of miR-199a decreased MMP-9 protein level. Interestingly, the neutralization of miR-199a by anti-miR-199a antibody reversed the effect of propofol on alleviation of tumor invasiveness and inhibition of MMP-9 activity in HepG2 cells. CONCLUSION: Propofol  decreases  hepatocellular  carcinoma cell invasiveness, which is partly due to the down-regulation of MMP-9 expression by miR-199a.

  2. 19 CFR 19.9 - General order, abandoned, and seized merchandise.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false General order, abandoned, and seized merchandise... Provisions § 19.9 General order, abandoned, and seized merchandise. (a) Acceptance of merchandise. The... Customs-authorized permit to transfer or in-bond entry) is responsible for preparing a Customs Form...

  3. 10 CFR 205.199A - Hearing for the purpose of oral argument only.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Hearing for the purpose of oral argument only. 205.199A... Hearing for the purpose of oral argument only. (a) A participant is entitled upon timely request to a... the purpose of receiving oral argument will generally be conducted only after the issues involved...

  4. A measurement of the neutron to 199Hg magnetic moment ratio

    Directory of Open Access Journals (Sweden)

    S. Afach

    2014-12-01

    Full Text Available The neutron gyromagnetic ratio has been measured relative to that of the 199Hg atom with an uncertainty of 0.8 ppm. We employed an apparatus where ultracold neutrons and mercury atoms are stored in the same volume and report the result γn/γHg=3.8424574(30.

  5. 32 CFR 199.20 - Continued Health Care Benefit Program (CHCBP).

    Science.gov (United States)

    2010-07-01

    ... deductible and cost sharing requirements and catastrophic cap limits, amounts applicable to the categories of... provisions of § 199.14 shall apply to the CHCBP as they do to CHAMPUS. (n) Peer Review Organization Program... organization programs available. Any preferred provider organization program under this part that provides...

  6. 46 CFR 199.175 - Survival craft and rescue boat equipment.

    Science.gov (United States)

    2010-10-01

    ... by the Federal Communications Commission as meeting the requirements of 47 CFR part 80 and is stowed... and specified for it in table 199.175 of this section under the vessel's category of service. A... stored in a waterproof container. (37) Thermal protective aid. The thermal protective aid must...

  7. Comparison of Plasma Tu-M2-PK and CA19-9 in Pancreatic Cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Heegaard, Niels H H; Schaffalitzky de Muckadell, Ove B

    2009-01-01

    because of suspicion of pancreatic cancer. Of these, 51 patients had their conditions diagnosed as PDAC, whereas this diagnosis was ruled out in 52 after 12 months of follow-up. The performance of Tu-M2-PK was compared with that of CA19-9 using cutoff values 15 and 37 U/mL, respectively. RESULTS:: The...

  8. A high precision comparison of the gyromagnetic ratios of the 199Hg atom and the neutron

    International Nuclear Information System (INIS)

    The current experimental search for the neutron electric dipole moment (EDM) uses a cohabiting magnetometer based upon measurements of the nuclear precession frequency of 199Hg atoms. This magnetometer reduces the systematic errors introduced into the measurement of the neutron EDM by changes in the magnetic field. In order to obtain a maximal reduction in the systematic errors, a precise value for the ratio of the neutron and 199Hg atom gyromagnetic ratios is required. This thesis describes a measurement of the ratio using a modified form of the neutron EDM apparatus. The result is found to be, γn/γ199Hg = -3.8425063 ± (0.5 x 10-6) ± (5.4 x 10-6) where the first error is due to statistics, and the second is due to systematics. Expressing this in terms of fractional errors we have: γn/γ199Hg = - 3.8425063 x (1 ± (1.3 x 10-7) ± (1.4 x 10-6)). This result represents a 15ppm shift in the value which may be derived from two previous experiments in the literature. The thesis also contains a review of the Rabi and Ramsey techniques of magnetic resonance. Some previously unpublished results are presented, including a derivation of a simple expression for the width of the Ramsey resonance lineshape, and the effect of having an oscillating magnetic field with a well defined initial phase. (author)

  9. Reduced Limit on the Permanent Electric Dipole Moment of $^{199}$Hg

    CERN Document Server

    Graner, B; Lindahl, E G; Heckel, B R

    2016-01-01

    This paper describes the results of the most recent measurement of the permanent electric dipole moment (EDM) of neutral $^{199}$Hg atoms. Fused silica vapor cells containing enriched $^{199}$Hg are arranged in a stack in a common magnetic field. Optical pumping is used to spin-polarize the atoms orthogonal to the applied magnetic field, and the Faraday rotation of near-resonant light is observed to determine an electric-field-induced perturbation to the Larmor precession frequency. Our results for this frequency shift are consistent with zero; we find the corresponding $^{199}$Hg EDM $d_{Hg} = (-2.20 \\pm 2.75_{stat} \\pm 1.48_{syst}) \\times 10^{-30} e\\cdot \\text{cm}$. We use this result to place a new upper limit on the $^{199}$Hg EDM $|d_{Hg}| < 7.4\\times 10^{-30} e\\cdot \\text{cm}$ (95\\% C.L.), improving our previous limit by a factor of 4. We also discuss the implications of this result for various $CP$-violating observables as they relate to theories of physics beyond the standard model.

  10. A measurement of the neutron to 199Hg magnetic moment ratio

    CERN Document Server

    Afach, S; Ban, G; Bison, G; Bodek, K; Burghoff, M; Chowdhuri, Z; Daum, M; Fertl, M; Franke, B; Geltenbort, P; Green, K; van der Grinten, M G D; Grujic, Z; Harris, P G; Heil, W; Hélaine, V; Henneck, R; Horras, M; Iaydjiev, P; Ivanov, S N; Kasprzak, M; Kermaïdic, Y; Kirch, K; Knecht, A; Koch, H -C; Krempel, J; Kuźniak, M; Lauss, B; Lefort, T; Lemière, Y; Mtchedlishvili, A; Naviliat-Cuncic, O; Pendlebury, J M; Perkowski, M; Pierre, E; Piegsa, F M; Pignol, G; Prashanth, P N; Quéméner, G; Rebreyend, D; Ries, D; Roccia, S; Schmidt-Wellenburg, P; Schnabel, A; Severijns, N; Shiers, D; Smith, K F; Voigt, J; Weis, A

    2014-01-01

    The neutron gyromagnetic ratio has been measured relative to that of the 199Hg atom with an uncertainty of 0.8 ppm. We employed an apparatus where ultracold neutrons and mercury atoms are stored in the same volume and report the result $\\gamma_{\\rm n}/\\gamma_{\\rm Hg} = 3.8424574(30)$.

  11. Frequency Ratio of ${}^{199}$Hg and ${}^{87}$Sr Optical Lattice Clocks beyond the SI Limit

    CERN Document Server

    Yamanaka, Kazuhiro; Ushijima, Ichiro; Takamoto, Masao; Katori, Hidetoshi

    2015-01-01

    We report on a frequency ratio measurement of a ${}^{199}$Hg-based optical lattice clock referencing a ${}^{87}$Sr-based clock. Evaluations of lattice light shift, including atomic-motion-dependent shift, enable us to achieve a total systematic uncertainty of $7.2 \\times 10^{-17}$ for the Hg clock. The frequency ratio is measured to be $\

  12. Reduced Limit on the Permanent Electric Dipole Moment of Hg 199

    Science.gov (United States)

    Graner, B.; Chen, Y.; Lindahl, E. G.; Heckel, B. R.

    2016-04-01

    This Letter describes the results of the most recent measurement of the permanent electric dipole moment (EDM) of neutral Hg 199 atoms. Fused silica vapor cells containing enriched Hg 199 are arranged in a stack in a common magnetic field. Optical pumping is used to spin polarize the atoms orthogonal to the applied magnetic field, and the Faraday rotation of near-resonant light is observed to determine an electric-field-induced perturbation to the Larmor precession frequency. Our results for this frequency shift are consistent with zero; we find the corresponding Hg 199 EDM dHg=(-2.20 ±2.7 5stat±1.4 8syst)×10-30e cm . We use this result to place a new upper limit on the Hg 199 EDM |dHg|<7.4 ×10-30e cm (95% C.L.), improving our previous limit by a factor of 4. We also discuss the implications of this result for various C P -violating observables as they relate to theories of physics beyond the standard model.

  13. Reduced Limit on the Permanent Electric Dipole Moment of ^{199}Hg.

    Science.gov (United States)

    Graner, B; Chen, Y; Lindahl, E G; Heckel, B R

    2016-04-22

    This Letter describes the results of the most recent measurement of the permanent electric dipole moment (EDM) of neutral ^{199}Hg atoms. Fused silica vapor cells containing enriched ^{199}Hg are arranged in a stack in a common magnetic field. Optical pumping is used to spin polarize the atoms orthogonal to the applied magnetic field, and the Faraday rotation of near-resonant light is observed to determine an electric-field-induced perturbation to the Larmor precession frequency. Our results for this frequency shift are consistent with zero; we find the corresponding ^{199}Hg EDM d_{Hg}=(-2.20±2.75_{stat}±1.48_{syst})×10^{-30}e cm. We use this result to place a new upper limit on the ^{199}Hg EDM |d_{Hg}|<7.4×10^{-30}e cm (95% C.L.), improving our previous limit by a factor of 4. We also discuss the implications of this result for various CP-violating observables as they relate to theories of physics beyond the standard model. PMID:27152789

  14. Comparison of circulating MMP-9, TIMP-1 and CA19-9 in the detection of pancreatic cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Brünner, Nils; De Muckadell, Ove B Schaffalitzky

    2010-01-01

    adenocarcinoma. PATIENTS AND METHODS: The patients had symptoms of pancreatic cancer. The discriminative strength of MMP-9 and TIMP-1 were compared to that of CA19-9 using receiver operating characteristics curves, area under the curves (AUC), specificity and sensitivity. RESULTS: The sensitivities of MMP-9......Background/Aim: The performance of the circulating tumor markers carbohydrate antigen 19-9 (CA19-9), matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) were evaluated separately and in combination for their potential value in detecting pancreatic ductal......, TIMP-1 and CA19-9 in detecting pancreatic ductal adenocarcinoma were 58.82%, 47.1% and 86%, respectively, with specificities of 34.6%, 69.2% and 73%. The AUCs of MMP-9, TIMP-1 and CA19-9 were 0.50, 0.64 and 0.84, respectively. Combining the three markers did not significantly improve detection of...

  15. Durable donor engraftment after radioimmunotherapy using α-emitter astatine-211-labeled anti-CD45 antibody for conditioning in allogeneic hematopoietic cell transplantation.

    Science.gov (United States)

    Chen, Yun; Kornblit, Brian; Hamlin, Donald K; Sale, George E; Santos, Erlinda B; Wilbur, D Scott; Storer, Barry E; Storb, Rainer; Sandmaier, Brenda M

    2012-02-01

    To reduce toxicity associated with external γ-beam radiation, we investigated radioimmunotherapy with an anti-CD45 mAb labeled with the α-emitter, astatine-211 ((211)At), as a conditioning regimen in dog leukocyte antigen-identical hematopoietic cell transplantation (HCT). Dose-finding studies in 6 dogs treated with 100 to 618 μCi/kg (211)At-labeled anti-CD45 mAb (0.5 mg/kg) without HCT rescue demonstrated dose-dependent myelosuppression with subsequent autologous recovery, and transient liver toxicity in dogs treated with (211)At doses less than or equal to 405 μCi/kg. Higher doses of (211)At induced clinical liver failure. Subsequently, 8 dogs were conditioned with 155 to 625 μCi/kg (211)At-labeled anti-CD45 mAb (0.5 mg/kg) before HCT with dog leukocyte antigen-identical bone marrow followed by a short course of cyclosporine and mycophenolate mofetil immunosuppression. Neutropenia (1-146 cells/μL), lymphopenia (0-270 cells/μL), and thrombocytopenia (1500-6560 platelets/μL) with prompt recovery was observed. Seven dogs had long-term donor mononuclear cell chimerism (19%-58%), whereas 1 dog treated with the lowest (211)At dose (155 μCi/kg) had low donor mononuclear cell chimerism (5%). At the end of follow-up (18-53 weeks), only transient liver toxicity and no renal toxicity had been observed. In conclusion, conditioning with (211)At-labeled anti-CD45 mAb is safe and efficacious and provides a platform for future clinical trials of nonmyeloablative transplantation with radioimmunotherapy-based conditioning. PMID:22134165

  16. Improvement on Synthesis Process of C. I. Direct Blue 199%C.I.直接蓝199工艺改进

    Institute of Scientific and Technical Information of China (English)

    王雪梅; 郑玉安

    2011-01-01

    The Synthesis Process of C. I. Direct Blue 199 was improved. The reaction temperature of CuPc and chtorosulfonic acid was raised from 130℃ to 140 ℃, then the reaction temperature of the product with thionyl chloride was raised from 60 - 65 ℃ to 80 ℃ ; the dropping time of thionyl chloride was prolonged from 2 - 2. 5 hours to 4 hours; and ammonia was replaced by organic base in ammonolysis. By this new technology, the difficult problem in membrane filtration before salting out was resolved when the dye was applied in ink.%对C.I.直接蓝199的老工艺进行了改进。使铜酞菁与氯磺酸的反应温度由原来的130℃升至140℃左右,之后与氯化亚砜的反应温度由60~65℃提高到80℃,而且滴加氯化亚砜的时间从2~2.5延长至4小时,同时氨解用的氨水用有机碱替代。新工艺解决了此染料用于墨水时在盐析前膜过滤困难的问题。

  17. Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

    Science.gov (United States)

    Gu, Nan; You, Lianghui; Shi, Chunmei; Yang, Lei; Pang, Lingxia; Cui, Xianwei; Ji, Chenbo; Zheng, Wen; Guo, Xirong

    2016-08-01

    Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses. PMID:27279151

  18. Targeting MCL-1/BCL-XL Forestalls the Acquisition of Resistance to ABT-199 in Acute Myeloid Leukemia

    Science.gov (United States)

    Lin, Kevin H.; Winter, Peter S.; Xie, Abigail; Roth, Cullen; Martz, Colin A.; Stein, Elizabeth M.; Anderson, Grace R.; Tingley, Jennifer P.; Wood, Kris C.

    2016-01-01

    ABT-199, a potent and selective small-molecule antagonist of BCL-2, is being clinically vetted as pharmacotherapy for the treatment of acute myeloid leukemia (AML). However, given that prolonged monotherapy tends to beget resistance, we sought to investigate the means by which resistance to ABT-199 might arise in AML and the extent to which those mechanisms might be preempted. Here we used a pathway-activating genetic screen to nominate MCL-1 and BCL-XL as potential nodes of resistance. We then characterized a panel of ABT-199-resistant myeloid leukemia cell lines derived through chronic exposure to ABT-199 and found that acquired drug resistance is indeed driven by the upregulation of MCL-1 and BCL-XL. By targeting MCL-1 and BCL-XL, resistant AML cell lines could be resensitized to ABT-199. Further, preemptively targeting MCL-1 and/or BCL-XL alongside administration of ABT-199 was capable of delaying or forestalling the acquisition of drug resistance. Collectively, these data suggest that in AML, (1) the selection of initial therapy dynamically templates the landscape of acquired resistance via modulation of MCL-1/BCL-XL and (2) appropriate selection of initial therapy may delay or altogether forestall the acquisition of resistance to ABT-199. PMID:27283158

  19. Biosorption of C.I. Direct Blue 199 from aqueous solution by nonviable Aspergillus niger

    International Nuclear Information System (INIS)

    The capacity and mechanism with which nonviable Aspergillus niger removed the textile dye, C.I. Direct Blue 199, from aqueous solution was investigated using different parameters, such as initial dye concentration, pH and temperature. In batch experiments, the biosorption capacity increased with decrease in pH, and the maximum dye uptake capacity of the biosorbent was 29.96 mg g-1 at 400 mg L-1 dye concentration and 45 deg. C. The Langmuir and Freundlich models were able to describe the biosorption equilibrium of C.I. Direct Blue 199 onto the fungal biomass. Biosorption followed a pseudo-second order kinetic model with high correlation coefficients (r2 > 0.99). Thermodynamic studies revealed that the biosorption process was successful, spontaneous and endothermic in nature.

  20. In-gas-cell laser spectroscopy for magnetic dipole moment of $^{199}$Pt toward $N=$ 126

    CERN Document Server

    Hirayama, Y; Watanabe, Y X; Jeong, S C; Jung, H S; Kakiguchi, Y; Kimura, S; Moon, J Y; Oyaizu, M; Park, J H; Schury, P; Wada, M; Miyatake, H

    2016-01-01

    Magnetic dipole moment and mean-square charge radius of $^{199}$Pt ($I^{\\pi}=$ 5/2$^-$) have been evaluated for the first time from the investigation of the hyperfine splitting of the $\\lambda_1=$ 248.792 nm transition by in-gas-cell laser ionization spectroscopy. Neutron-rich nucleus $^{199}$Pt was produced by multi-nucleon transfer reaction at the KISS where the nuclear spectroscopy in the vicinity of $N=$ 126 is planed from the aspect of an astrophysical interest as well as the nuclear structure. Measured magnetic dipole moment $+$0.63(13)$\\mu_{\\rm N}$ is consistent with the systematics of those of nuclei with $I^{\\pi}=$ 5/2$^-$. The deformation parameter $|^{1/2}|$ evaluated from the isotope shift indicates the gradual shape change to spherical shape of platinum isotopes with increasing neutron number toward $N=$ 126.

  1. CA 242, a new tumour marker for pancreatic cancer: a comparison with CA 19-9, CA 50 and CEA.

    OpenAIRE

    Haglund, C.; Lundin, J; Kuusela, P.; Roberts, P. J.

    1994-01-01

    The serum expression of a novel tumour marker, CA 242, defined by monoclonal antibody C 242, was studied in 179 patients with pancreatic cancer. The results were compared with CA 19-9, CA 50 and CEA. CA 242 is a carbohydrate closely related, but not identical, to CA 19-9 and CA 50. The overall sensitivity of the CA 242 assay was 74%: 55% in stage I, 83% in stage II-III and 78% in stage IV disease. The specificity calculated from 112 patients with benign diseases was 91%. CA 19-9 had a higher ...

  2. Magnetic rotations in 198Pb and 199Pb within covariant density functional theory

    OpenAIRE

    Yu, L. F.; Zhao, P. W.; Zhang, S.Q.; Ring, P.; Meng, J

    2011-01-01

    Well-known examples of shears bands in the nuclei 198Pb and 199Pb are investigated within tilted axis cranking relativistic mean-field theory. Energy spectra, the relation between spin and rotational frequency, deformation parameters and reduced $M1$ and $E2$ transition probabilities are calculated. The results are in good agreement with available data and with calculations based on the phenomenological pairing plus-quadrupole-quadrupole tilted-axis cranking model. It is shown that covariant ...

  3. Serum CA 19-9 as a Biomarker for Pancreatic Cancer—A Comprehensive Review

    OpenAIRE

    Ballehaninna, Umashankar K; Chamberlain, Ronald S.

    2011-01-01

    Pancreatic cancer is an aggressive tumor with a dismal prognosis, biomarkers that can detect tumor in its early stages when it may be amenable to curative resection may improve prognosis. At present, serum CA 19-9 is the only validated tumor marker in widespread clinical use, but precise knowledge of its role in pancreatic cancer diagnosis, staging, determining resectability, response to chemotherapy and prognosis remains limited. A comprehensive search was performed using PubMed with keyword...

  4. The two inner β-transitions in the decay of 199Au

    International Nuclear Information System (INIS)

    The directional correlations of the β(302 keV) - γ(158 keV) and β(251 keV) - γ(208 keV) cascades in 199Au decay are measured and found to be isotropic, consistent with the validity of Ψ-approximation for both the β-transitions. These results are also in accordance with the core-excitation model. (author)

  5. Gold Nanoparticles Doped with (199) Au Atoms and Their Use for Targeted Cancer Imaging by SPECT.

    Science.gov (United States)

    Zhao, Yongfeng; Pang, Bo; Luehmann, Hannah; Detering, Lisa; Yang, Xuan; Sultan, Deborah; Harpstrite, Scott; Sharma, Vijay; Cutler, Cathy S; Xia, Younan; Liu, Yongjian

    2016-04-01

    Gold nanoparticles have been labeled with various radionuclides and extensively explored for single photon emission computed tomography (SPECT) in the context of cancer diagnosis. The stability of most radiolabels, however, still needs to be improved for accurate detection of cancer biomarkers and thereby monitoring of tumor progression and metastasis. Here, the first synthesis of Au nanoparticles doped with (199) Au atoms for targeted SPECT tumor imaging in a mouse triple negative breast cancer (TNBC) model is reported. By directly incorporating (199) Au atoms into the crystal lattice of each Au nanoparticle, the stability of the radiolabel can be ensured. The synthetic procedure also allows for a precise control over both the radiochemistry and particle size. When conjugated with D-Ala1-peptide T-amide, the Au nanoparticles doped with (199) Au atoms can serve as a C-C chemokine receptor 5 (CCR5)-targeted nanoprobe for the sensitive and specific detection of both TNBC and its metastasis in a mouse tumor model. PMID:26865221

  6. Relationship between serum calcium and CA 19-9 levels in colorectal cancer

    Institute of Scientific and Technical Information of China (English)

    Peter Fuszek; Peter Lakatos; Adam Tabak; Janos Papp; Zsolt Nagy; Istvan Takacs; Henrik Csaba Horvath; Peter Laszlo Lakatos; Gabor Speer

    2004-01-01

    AIM: To examine the calcium metabolism of colorectal cancer(CRC) in patients with colorectal cancer and control patients.METHODS: Seventy newly diagnosed CRC patients were included. The healthy control group was age and gender matched (n=32). Particular attention was devoted to the relationship between serum calcium of patients, and levels of AFP, CEA, carbohydrate antigen 19-9 (CA 19-9) (that could be considered as prognostic factors). Furthermore, the Ca-sensing receptor (CaSR) gene A986S polymorphism was investigated in these patients, as well as the relationship between different CaSR genotypes and the data stated above.RESULTS: A lower level of ionized calcium (also corrected for albumin) was found in the serum of CRC patients with normal 25(OH) vitamin D levels. The ionized calcium concentration was inversely correlated with the serum level of CA 19-9. There was no difference in the distribution of CaSR genotypes, between CRC patients and general population. The genotypes did not correlate with other data examined.CONCLUSION: Based on these results, lower levels of serum calcium might be a pathogenic and prognostic factor in colorectal cancer.

  7. CA19-9 Levels in Saliva and Urine of Patients with Ductal Adenocarcinoma of the Pancreas Correlates with Serum CA19-9 Levels - A Prospective Preliminary Study

    Directory of Open Access Journals (Sweden)

    Offir Ben-Ishay

    2016-08-01

    Full Text Available Context Ductal adenocarcinoma of the pancreas is the fifth leading cause of cancer related deaths in the European Union. Tumor markers CA19-9 and carcinoembryonic antigen are important components in decision making and follow-up of patients diagnosed with this disease. These tumor markers were found to be elevated not only in the serum but also in other body fluids in patients with malignant lesions of the parotid gland and the urinary tract. Objectives The current preliminary study evaluates the correlation between serum CA19-9 and carcinoembryonic antigen levels with those obtained in saliva and urine of patients with ductal adenocarcinoma of the pancreas. Methods We have prospectively collected blood, urine and saliva of patients with biopsy proven ductal adenocarcinoma of the pancreas and documented elevated serum CA19-9 and/or carcinoembryonic antigen levels. In addition we have collected body fluids of healthy controls for normal values levels definition. Levels of tumor markers were measured using an immunochemistry analyzer based on an electrochemiluminescence sandwich immunoassay. Results CA19-9 and carcinoembryonic antigen were both detected in the saliva and urine of patients and controls. CA19-9 levels of the healthy controls were normal with a significant increase in serum CA19-9 (p-0.008 of the patient's group. Pearson product moment correlation test demonstrated a linear and consistent correlation between serum CA19-9 and saliva and urine levels. Conclusions The current pilot study demonstrated linear correlation of serum levels of CA19-9 with those measured in the saliva and urine of patients with ductal adenocarcinoma of the pancreas. These results encourage us to undertake a larger cohort study to confirm our results and standardize the detected levels in these body fluids.

  8. Identification of a Novel Human Papillomavirus, Type HPV199, Isolated from a Nasopharynx and Anal Canal, and Complete Genomic Characterization of Papillomavirus Species Gamma-12

    OpenAIRE

    Oštrbenk, Anja; Kocjan, Boštjan J.; Hošnjak, Lea; Li, Jingjing; Deng, Qiuju; Šterbenc, Anja; Poljak, Mario

    2015-01-01

    The novel human papillomavirus type 199 (HPV199) was initially identified in a nasopharyngeal swab sample obtained from a 25 year-old immunocompetent male. The complete genome of HPV199 is 7,184 bp in length with a GC content of 36.5%. Comparative genomic characterization of HPV199 and its closest relatives showed the classical genomic organization of Gammapapillomaviruses (Gamma-PVs). HPV199 has seven major open reading frames (ORFs), encoding five early (E1, E2, E4, E6, and E7) and two late...

  9. Marcador tumoral CA 19.9 aumentado sin evidencia de malignidad Elevated Ca 19.9 tumor marker without evidence of malignancy

    Directory of Open Access Journals (Sweden)

    Eduardo González Bosquet

    2007-06-01

    Full Text Available Los marcadores tumorales son de gran utilidad clínica en el seguimiento de los pacientes oncológicos. Su papel en el diagnóstico de tumoraciones malignas es controvertido. Presentamos un caso de una mujer con un quiste ovárico benigno en la cual los marcadores aumentan de forma inexplicable después de la extirpación del mismo.Tumor markers are a useful tool for surveillance of oncologic patients, whereas their role in the diagnosis of a malignancy is controversial. We present the case of a woman with a benign ovarian cyst with an unexpected elevation of Ca 19.9 after laparoscopic bilateral anexectomy.

  10. Correlation factor analysis of mature ovarian teratom and serum CA199 and serum CA125%卵巢成熟畸胎瘤与糖抗原199、125相关分析

    Institute of Scientific and Technical Information of China (English)

    黄云霞; 徐妍婷; 尤志群

    2013-01-01

    目的:探讨CA199、CA125与卵巢成熟畸胎瘤的相关性及对其诊断和鉴别诊断价值。方法:分析卵巢成熟畸胎瘤276例患者年龄、术前CA199、CA125检测值、术中肿瘤的数量、大小、分布情况及相关性。结果:(1)276例中CA199阳性85例(30.80%);CA125阳性34例(12.32%);两者均为阳性者18例(6.5%)。两者阳性数比较差异有高度统计学意义(P<0.001)。(2)成熟畸胎瘤单侧236例,CA199阳性63例,CA125阳性27例。双侧成熟畸胎瘤40例中CA199阳性22例,CA125阳性7例。(3)平均体积为159.33±221.11cm3,瘤体直径>10cm 31例,6~10cm 102例,≤5cm 143例,瘤体直径越大,阳性检出率越高。(4)不同年龄段CA199、CA125的阳性率呈降低趋势,年龄越小,CA199、CA125阳性率越高。结论:卵巢成熟畸胎瘤可导致血清CA199、CA125血清表达升高,但CA199检测有更高临床意义。%Objective:To investigate the correlation between mature ovarian teratoma and the levels of CA199 and CA125. Method:The correlation among the factors including ages, values of CA199 and CA125 before the surgery, and the numbers, sizes and distribution of the tumours during the surgery of 276 patients suffering from mature ovarian teratomas was retrospectively analysed. Result: The overall rates for CA199 and CA125 in the serum tested positive were 30.80%and 12.32%, respectively, which indicated correlation between the numbers, sizes and distribution of the teratomas and the ages of patients. The younger the patients with larger volumes of teratomas or/and bilateral teratomas of ovaries, the higher the level of CA199 and CA125. Conclusion: The detection of CA199 and CA125 is advantageous in the diagnoses and i-dentification of mature ovarian teratomas. However, the detection of CA199 is of more clinical value.

  11. Clinical analysis of the value of combined detection of serum CA199 and CA50 levels in patients with pancreatic cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical value of combined test of serum CA199 and CA50 levels in the diagnosis and prognosis of patients with pancreatic cancer. Methods: Serum levels of CA199 and CA50 in 40 cases of pancreatic cancer, 20 cases of chronic pancreatitis and 30 normal controls were detected by radioimmunoassay. Results: Serum CA199 and CA50 levels in pancreatic cancer patients were significantly higher than those in normal controls and chronic pancreatitis patients (P 199 and CA50 were detected simultaneously, the sensitivity, specificity and accuracy of diagnosis of pancreatic cancer could be improved. Serum CA199 and CA50 levels in cancer patients with lymph node metastasis were much higher than those without lymph node metastasis (P 199 and CA50 detected simultaneously could improve the sensitivity and accuracy in diagnosis of pancreatic cancer. The changes of serum CA199 and CA50 level were helpful to predict whether metastasis or recurrence had occurred

  12. ABT-199 mediated inhibition of BCL-2 as a novel therapeutic strategy in T-cell acute lymphoblastic leukemia.

    Science.gov (United States)

    Peirs, Sofie; Matthijssens, Filip; Goossens, Steven; Van de Walle, Inge; Ruggero, Katia; de Bock, Charles E; Degryse, Sandrine; Canté-Barrett, Kirsten; Briot, Delphine; Clappier, Emmanuelle; Lammens, Tim; De Moerloose, Barbara; Benoit, Yves; Poppe, Bruce; Meijerink, Jules P; Cools, Jan; Soulier, Jean; Rabbitts, Terence H; Taghon, Tom; Speleman, Frank; Van Vlierberghe, Pieter

    2014-12-11

    T-cell acute lymphoblastic leukemia (T-ALL) is a high-risk subtype of acute lymphoblastic leukemia (ALL) with gradually improved survival through introduction of intensified chemotherapy. However, therapy-resistant or refractory T-ALL remains a major clinical challenge. Here, we evaluated B-cell lymphoma (BCL)-2 inhibition by the BH3 mimetic ABT-199 as a new therapeutic strategy in human T-ALL. The T-ALL cell line LOUCY, which shows a transcriptional program related to immature T-ALL, exhibited high in vitro and in vivo sensitivity for ABT-199 in correspondence with high levels of BCL-2. In addition, ABT-199 showed synergistic therapeutic effects with different chemotherapeutic agents including doxorubicin, l-asparaginase, and dexamethasone. Furthermore, in vitro analysis of primary patient samples indicated that some immature, TLX3- or HOXA-positive primary T-ALLs are highly sensitive to BCL-2 inhibition, whereas TAL1 driven tumors mostly showed poor ABT-199 responses. Because BCL-2 shows high expression in early T-cell precursors and gradually decreases during normal T-cell differentiation, differences in ABT-199 sensitivity could partially be mediated by distinct stages of differentiation arrest between different molecular genetic subtypes of human T-ALL. In conclusion, our study highlights BCL-2 as an attractive molecular target in specific subtypes of human T-ALL that could be exploited by ABT-199. PMID:25301704

  13. 小胰腺癌血清CA19-9测定及其临床意义%Detection and Clinical Significance of Serum CA19-9 for Small Pancreas Cancer

    Institute of Scientific and Technical Information of China (English)

    徐慧琴

    2001-01-01

    Objective: To determine the significance of serum CA19-9 for small pancreas cancer.Methods: The serum CA19-9 of 86 patients of pancreas cancer was detected. 18 patients of small pancreas cancer whose tumor diameter were less than 2 cm were received B ultrasound, CT, MRI and ERCP. Results: The serum CA19-9 positive rate was 61.1% in the patients of small pancreas cancer with its average value 140.5u/ml. The CA19-9 positive was common in the patient of small pancreas cancer with the lesion focus metastasis. There was no correlation between the serum CA19-9 and stage and tumor site of small pancreas cancer. Conclusion: The detection of serum CA19-9 might be helpful for the assessment of operative effect and supervision of follow-up after operation. The diagnostic positive rate of MRI and ERCP for small pancreas cancer should be higher than that of B ultrasound and CT. The combination detections of the serum CA19-9, MRI and ERCP could reach the purpose of early diagnosis.Early operation could reduce the mortality and improve the prognosis.%目的:明确血清CA19-9测定对小胰腺癌诊断的意义.方法:通过对86例胰腺癌患者血清CA19-9的检测,并对其中18例肿瘤直径≤2cm小胰腺癌)患者进行B超、CT、MRI及ERCP检测.结果:结果表明小胰腺癌患者血清CA19-9的阳性率为61.1%,平均值为140.5u/ml,病理显示CA19-9阳性多见于病灶有转移的小胰腺癌病人.血清CA19-9的阳性与小胰腺癌的病程,部位无关.结论:CA19-9检测有助于对手术疗效的评价与术后随访的监测.MRI、ERCP对小胰腺癌诊断阳性率明显高于B超、CT.联合应用血清CA19-9、MRI、ERCP 可达到早期诊断小胰腺癌的目的,而早期手术可降低死亡率,改善预后.

  14. Comparison of leaf proteomes of cassava (Manihot esculenta Crantz cultivar NZ199 diploid and autotetraploid genotypes.

    Directory of Open Access Journals (Sweden)

    Feifei An

    Full Text Available Cassava polyploid breeding has drastically improved our knowledge on increasing root yield and its significant tolerance to stresses. In polyploid cassava plants, increases in DNA content highly affect cell volumes and anatomical structures. However, the mechanism of this effect is poorly understood. The purpose of the present study was to compare and validate the changes between cassava cultivar NZ199 diploid and autotetraploid at proteomic levels. The results showed that leaf proteome of cassava cultivar NZ199 diploid was clearly differentiated from its autotetraploid genotype using 2-DE combined MS technique. Sixty-five differential protein spots were seen in 2-DE image of autotetraploid genotype in comparison with that of diploid. Fifty-two proteins were identified by MALDI-TOF-MS/MS, of which 47 were up-regulated and 5 were down-regulated in autotetraploid genotype compared with diploid genotype. The classified functions of 32 up-regulated proteins were associated with photosynthesis, defense system, hydrocyanic acid (HCN metabolism, protein biosynthesis, chaperones, amino acid metabolism and signal transduction. The remarkable variation in photosynthetic activity, HCN content and resistance to salt stress between diploid and autotetraploid genotypes is closely linked with expression levels of proteomic profiles. The analysis of protein interaction networks indicated there are direct interactions between the 15 up-regulation proteins involved in the pathways described above. This work provides an insight into understanding the protein regulation mechanism of cassava polyploid genotype, and gives a clue to improve cassava polyploidy breeding in increasing photosynthesis and resistance efficiencies.

  15. Benign Hydronephrosis and Elevated of Serum Levels of Carbohydrate Antigen CA 19-9: A Case Report.

    Science.gov (United States)

    Filipovic, Branka; Milinić, Nikola; Gacic, Jasna; Markovic, Olivera; Djokovic, Aleksandra; Filipovic, Branislav

    2016-01-01

    BACKGROUND Carbohydrate tumor-associated antigen (CA 19-9) has been shown to be upregulated in other malignant tumors including gastric, ovarian, hepatocellular, and colorectal carcinoma as well as benign diseases of the biliary track such as pancreatitis, cholangitis, and choledocholithiasis. According to the available literature, in several cases of benign hydronephrosis and in a few cases of benign renal diseases, elevated CA 19-9 has been noted. CASE REPORT A 58-year-old Caucasian male patient was admitted in our clinic with complaints about blunt abdominal pain in the past two-month period localized in the right lumbar region and irradiating into the right inguinal area, constipation, abdominal bloating, and intermittent hematuria. The concentration of serum CA 19-9 was 3500 U/mL. Urine cytology provided no signs of abnormality. Intravenous urography visualized right-sided pyelon and ureter duplex with the defect in contrast shade of the pyelon, caused by a stag horn calculus. Contrast added computerized axial tomography of the abdomen and pelvis visualized the pyelon casted concretion spreading throughout the right pyelon, with ureterohydronephrosis with the distal block for passage of the contrast to the distal part of the ureter. CONCLUSIONS There is no doubt that CA 19-9 level is occasionally elevated in patients with obstructive urolithiasis as it was in our case. In the routine medical praxis, urolithiasis should not be neglected in the differential diagnosis of elevated concentrations of CA 19-9 marker. PMID:27287959

  16. Combined detection of preoperative serum CEA, CA19-9 and CA242 improve prognostic prediction of surgically treated colorectal cancer patients

    OpenAIRE

    Wang, Jingtao; Xiao WANG; Yu, Fudong; Chen, Jian; Zhao, Senlin; Zhang, Dongyuan; YU, Yang; Liu, Xisheng; Tang, Huamei; Peng, Zhihai

    2015-01-01

    We assessed the prognostic significance of preoperative serum carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9) and carbohydrate antigen 242 (CA242) levels in surgically treated colorectal cancer patients. The relationship of preoperative serum CEA, CA19-9 and CA242 levels with disease characteristics was investigated in 310 patients. Correlation between tumor markers was investigated using Pearson correlation test. Univariate and multivariate survival analyses were used to s...

  17. AFP、CEA、CA199联合检测在原发性肝癌疾病诊疗中的应用

    Institute of Scientific and Technical Information of China (English)

    姜敬学

    2016-01-01

    目的::分析探讨原发性肝癌应用联合甲胎蛋白(α-fetoprotein,AFP)、癌胚抗原( carcino-embryonic antigen, CEA)及糖类抗原199(carbohydrate antigen,CA199)检查的诊断价值。方法:以122例疑似原发性肝癌患者为观察对象,所有患者均接受AFP、CEA、CA199及病理学检查,以病理学检查结果作为原发性肝癌的确诊标准,分别对单纯AFP、CEA、CA199及联合AFP、CEA、CA199检查对原发性肝癌的诊断价值进行分析。结果:101例患者明确诊断为原发性肝癌,联合AFP、CEA、CA199检查的灵敏性及特异性均明显高于单纯AFP、CEA及CA199检查;且单纯AFP、CEA、CA199及联合AFP、CEA、CA199检查对于原发性肝癌均有着显著的诊断价值,但联合AFP、CEA、CA199检查诊断价值明显高于单纯AFP、CEA及CA199检查。结论:联合AFP、CEA及CA199检查对于原发性肝癌有着较高的诊断价值。

  18. A meaningful appraisal of cholestasis in serum total bilirubin, cholyglyine, alpha-fetoprotein and carbohydrate antigen19-9

    International Nuclear Information System (INIS)

    Objective: Appraise the clinical significant how the serum total bilirubin (TB), cholylglycine (CG), α-fetoprotein (AFP), carbohydrate antigen 19-9 (CA 19-9) have their concentration changes in the pathological changes of intrahepatic cholestasis through a combined detection to them. Methods: The serum samples from 96 cases of chronic virus hepatitis, 26 cases of liver cirrhosis and 50 cases of normal people were detected by biochemistry for TB, by radioimmunoassay for CG, by electro chemiluminescence for AFP and CA19-9. Results: There is no obvious deference of serum TB in the group without intrahepatic cholestasis, the group of cholestasis without clinical symptoms and the control groups. There is also a marked deference (P<0.01) in the group of cholestasis with clinical symptoms, the group of liver cirrhosis, the group without intrahepatic cholestasis, the control group and the group of cholestasis without clinical symptoms. the serum CG from the groups of intrahepatic cholestasis, the group without intrahepatic cholestasis, and the control group all show a very obvious deference (P<0.01). The serum CA19-9 from the groups of intrahepatic cholestasis and the group without intrahepatic cholestasis show an obvious deference. The serum AFP, CA19-9 from the group of liver cancer show a very obvious deference (P<0.01). Conclusions: In clinc bilirubin is a rough index to reflect cholestasis. It has its own limit in deciding patterns of deferent bile obstruction. In the early stage of intrahepatic cholestasis, that the index of CG is high obviously points out existence of intrahepatic cholestasis. CG and the liver impairment are well interrelated and they are comparatively sensitive indexes of liver function. AFP reflects the regeneration of the liver cell necrosis and it means alarm to the seriousness of intrahepatic cholestasis. CA19-9 is a marker of tumor of biliary tract. The index increase through an initial observation is interrelated to the seriousness of

  19. Tumor markers CA19-9, CA242 and CEA in the diagnosis of pancreatic cancer: a meta-analysis

    OpenAIRE

    Zhang, Yimin; Yang, Jun; Li, Hongjuan; WU, YIHUA; Zhang, Honghe; Chen, Wenhu

    2015-01-01

    Background: Pancreatic cancer has the worst prognosis and early detection is crucial for improving patient prognosis. Therefore, we performed a meta-analysis to evaluate and compare the sensitivity and specificity of single test of CA19-9, CA242, and CEA, as well as combination test in pancreatic cancer detection. Methods: We searched PubMed, Embase, Medline, and Wanfang databases for studies that evaluated the diagnostic validity of CA19-9, CA242, and CEA between January 1990 and September 2...

  20. Adjusting CA19-9 values to predict malignancy in obstructive jaundice: Influence of bilirubin and C-reactive protein

    Institute of Scientific and Technical Information of China (English)

    Gaetano La Greca; Maria Sofia; Rosario Lombardo; Saverio Latteri; Agostino Ricotta; Stefano Puleo; Domenico Russello

    2012-01-01

    AIM:To find a possible relationship between inflammation and CA19-9 tumor marker by analyzing data from patients with benign jaundice (BJ) and malignant jaundice (MJ).METHODS:All patients admitted for obstructive jaundice,in the period 2005-2009,were prospectively enrolled in the study,obtaining a total of 102 patients.On admission,all patients underwent complete standard blood test examinations including C-reactive protein (CRP),bilirubin,CA19-9.Patients were considered eligible for the study when they presented obstructive jaundice confirmed by instrumental examinations and increased serum bilirubin levels (total bilirubin > 2.0 mg/dL).The standard cut-off level for CA19-9 was 32 U/mL,whereas for CRP this was 1.5 mg/L.The CA19-9 level was adjusted by dividing it by the value of serum bilirubin or by the CRP value.The patients were divided into 2 groups,MJ and BJ,and after the adjustment a comparison between the 2 groups of patients was performed.Sensitivity,specificity and positive predictive values were calculated before and after the adjustment.RESULTS:Of the 102 patients,51 were affected by BJ and 51 by MJ.Pathologic CA19-9 levels were found in 71.7% of the patients.In the group of 51 BJ patients there were 29 (56.9%) males and 22 (43.1%) females with a median age of 66 years (range 24-96 years),whereas in the MJ group there were 24 (47%) males and 27 (53%) females,with a mean age of 70 years (range 30-92 years).Pathologic CA19-9 serum level was found in 82.3% of MJ.CRP levels were pathologic in 66.6% of the patients with BJ and in 49% with MJ.Bilirubin and CA19-9 average levels were significantly higher in MJ compared with BJ (P =0.000 and P =0.02),while the CRP level was significantly higher in BJ (P =0.000).Considering a CA19-9 cut-off level of 32 U/mL,82.3% in the MJ group and 54.9% in the BJ group were positive for CA19-9 (P =0.002).A CA19-9 cut-off of 100 U/mL increases the difference between the two groups:35.3% in BJ and 68.6

  1. Magnetic rotations in 198Pb and 199Pb within covariant density functional theory

    CERN Document Server

    Yu, L F; Zhang, S Q; Ring, P; Meng, J

    2011-01-01

    Well-known examples of shears bands in the nuclei 198Pb and 199Pb are investigated within tilted axis cranking relativistic mean-field theory. Energy spectra, the relation between spin and rotational frequency, deformation parameters, and reduced M1 and E2 transition probabilities are calculated. The results are in good agreement with available data and with calculations based on the phenomenological pairing plus-quadrupole-quadrupole tilted axis cranking model. It is shown that covariant density functional theory provides a successful microscopic and fully self-consistent description of magnetic rotation in the Pb region showing the characteristic properties as the shears mechanism and relatively large B(M1) transitions decreasing with increasing spin.

  2. 三星CDMA X199工程模式

    Institute of Scientific and Technical Information of China (English)

    飞贼

    2003-01-01

    COMA手机一直在这个栏目较少介绍,但随着CDMA通信技术在国内的发展日臻成熟,我们将尽力加大这部分手机使用的介绍,满足广大读者的需要。今天我们要介绍的主角是三星的X199,相信关注CDMA手机的用户对它已经有所了解,所以这里就给大家说一说它鲜为人知的工程模式。

  3. High spin levels in the 199Pb and 200Pb isotopes

    International Nuclear Information System (INIS)

    The de-excitation of the 200Pb and 199Pb isotopes, produced through the 186W(18O 4n, 5n) reactions, is investigated using γ-ray and conversion electron spectroscopy. The conversion coefficients are measured and the multipolarities deduced for a great number of transitions. The e--γ and γ-γ coincidences and the half-life measurements lead to several new levels in both level schemes: the 200Pb one is extended up to nearly 7 MeV, where an isomer is found. The experimental results are compared to those of microscopic calculations, performed in a two or three quasi-particle approximation, using a surface delta interaction with a reduced pairing component

  4. Detection of hTERT mRNA, CEA, CA19-9 in Pleural Effusion and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    Niangui Zhao; Xiaoqun Ye; Weilan Yang; Yeqing Zou; Chunsong Yan

    2008-01-01

    OBJECTIVE To explore the clinical significance of the human telomerase reverse transcriptase (hTERT) mRNA, CEA and CA19-9 in differential diagnosis of benign and malignant pleural effusions.METHODS Concentrations of CEA and CA19-9 in pleural effusions were assayed using automated chemiluminescence, and expression of hTERT mRNA was detected by RT-PCR.RESULTS The positive rates of hTERT mRNA, CEA and CA19-9 expression in the group with malignant effusions were significantly higher compared to the group with benign effusion(P < 0.05). The sensitivity (%), specificity (%) and diagnostic accordance rates (%) of the 3 tumor markers were as follows: I)hTERT mRNA: 81.8/90.5/86.1; ii) CEA: 52.3/92.9/72.1; iii) CA19-9:34.1/90.5/61.6. The positive rates of hTERT mRNA + CEA (%)expression in the pleural effusions were 97.7.CONCLUSION All of these tumor markers can be helpful for differential diagnosis of pleural effusions, hTERT mRNA had more clinical value in differentiation of the pleural efffusions.CA19-9 is unfit to be as an optimal index. The combined assay of hTERT mRNA and CEA in pleural effusions can further raise the positive detection rate of the tumor markers and can be helpful in producing a diagnosis.

  5. 41 CFR 101-25.500 - Cross-reference to the Federal Acquisition Regulation (FAR) (48 CFR chapter 1, parts 1-99).

    Science.gov (United States)

    2010-07-01

    ... Federal Acquisition Regulation (FAR) (48 CFR chapter 1, parts 1-99). 101-25.500 Section 101-25.500 Public...-reference to the Federal Acquisition Regulation (FAR) (48 CFR chapter 1, parts 1-99). For guidance see Federal Acquisition Regulation Subpart 7.4 (48 CFR Subpart 7.4)....

  6. Platinum determination by instrumental neutron activation analysis with special reference to the spectral interference of Sc-47 on the platinum indicator nuclide Au-199

    DEFF Research Database (Denmark)

    Alfassi, Z.B.; Probst, T.U.; Rietz, B.

    A method of instrumental neutron activation analysis (INAA) is developed for the determination of platinum by the Au-199 daughter of Pt-199 in the presence of the spectral interference from the Sc-47 daughter of Ca-47. The contributions of the Pt and Ca signals to the integral 157-161 keV peak were...

  7. Cisplatin-induced downregulation of miR-199a-5p increases drug resistance by activating autophagy in HCC cell

    International Nuclear Information System (INIS)

    Highlights: ► miR-199a-5p levels were significantly decreased after cisplatin treatment. ► Cisplatin treatment induced autophagy activation. ► Cisplatin-induced downregulation of miR-199a-5p increases drug resistance by activating autophagy in HCC cell. -- Abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Systemic chemotherapy plays an important role in the treatment of patients with advanced liver cancer. However, chemoresistance to cisplatin is a major limitation of cisplatin-based chemotherapy in the clinic, and the underlying mechanism of such resistance is not fully understood. In the study, we found that miR-199a-5p levels were significantly reduced in HCC patients treated with cisplatin-based chemotherapy. Cisplatin treatment also resulted in decreased miR-199a-5p levels in human HCC cell lines. Forced expression of miR-199a-5p promoted cisplatin-induced inhibition of cell proliferation. Cisplatin treatment activated autophagy in Huh7 and HepG2 cells, which increased cell proliferation. We further demonstrated that downregulated miR-199a-5p enhanced autophagy activation by targeting autophagy-associated gene 7 (ATG7). More important, autophagy inhibition abrogated miR-199a-5p downregulation-induced cell proliferation. These data demonstrated that miR-199a-5p/autophagy signaling represents a novel pathway regulating chemoresistance, thus offering a new target for chemotherapy of HCC.

  8. Diagnostic value of combined determination of serum CA19-9 and TGF-β contents in patients with pancreatic cancer

    International Nuclear Information System (INIS)

    Objective: To study the clinical diagnostic value of combined determination of serum contents of CA19-9 and TGF-β in patients with pancreatic cancer. Methods: Serum CA19-9 (with RIA) and TGF-β (with ELISA) contents were deter- mined in 30 patients with pancreatic cancer and 35 controls. Results: The serum CA19-9 and TGF-β contents in patients with pancreatic cancer were significantly higher than those in controls (P<0.01). The diagnostic sensitivity of CA19-9 for pancreatic cancer was 70.8%, lower than that of TGF-β (80.2%, P<0.05). The diagnostic specificity of CA19-9 and TGF-β was 90.2% and 93.4% respectively. Conclusion: Both determinations of serum CA19-9 and TGF-β contents would yield high specificity for diagnosis of pancreatic cancer. Sensitity of TGF-β determination was higher than that of CA19-9 determination. Combined determination of CA19-9 and TGF-β would improve the diagnostic accuracy in patients with pancreatic cancer. (authors)

  9. Clinical significance of determination of changes of serum SIL-2R , TNF-α and CA199 levels after treatment in patients with endometriosis

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical significance of changes of serum SIL-2R, TNF-α and CA199 levels after treatment in patients with endometriosis. Methods: Serum TNF-α and CA199 levels were detected with RIA and SIL- 2R levels with ELISA in 40 patients with endometriosis both before and after treatment and 35 controls. Results: Before treatment, the serum SIL-2R, CA199 and TNF-α levels were significantly higher in patients than those in controls (P<0.01). After treatment for one month. The serum SIL-2R, TNF-α and CA199 levels were much corrected but still remained significantly higher than those in the controls (P<0.05). Conclusion: Determination of serum SIL-2R, TNF-α and CA199 levels in clinically was useful in the management in patients with endometriosis. (authors)

  10. Clinical significance of determination of changes of plasma ET and serum TNF-α, CA19-9 levels after treatment in patients with endometriosis

    International Nuclear Information System (INIS)

    Objective: To explore the clinical significance of changes of plasma ET and serum TNF-α, CA19-9 levels after treatment in patients with endometriosis. Methods: Plasma ET and Serum TNF-α, CA19-9 levels were detected with RIA in 38 patients with endometriosis both before and after treatment and 35 controls. Results: Before treatment, the plasma ET and serum TNF-α, CA19-9 levels were significantly higher in the patients than those in controls (P0.05). There were significantly positive correlation between the levels of plasma ET and serum TNF-α, CA19-9 levels (r=0.6118, 0.6014, P<0.01). Conclusion: Determination of plasma ET and serum TNF-α, CA19-9 levels in clinically useful in the management of patients with endometriosis. (authors)

  11. o199sc.m77t and o199sc.h77t: MGD77T data and header file for single-beam bathymetry for field activity O-1-99-SC in Southern California from 06/05/1999 to 06/17/1999

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Single-beam bathymetry data along with DGPS navigation data was collected as part of the U.S. Geological Survey cruise O-1-99-SC. The cruise was conducted in...

  12. Treatment of cultured glioma cells with the EGFR-TKI gefitinib (''Iressa'', ZD1839) increases the uptake of astatinated EGF despite the absence of gefitinib-mediated growth inhibition

    International Nuclear Information System (INIS)

    The EGFR-TKI (epidermal growth factor receptor tyrosine kinase inhibitor) gefitinib (''Iressa'', ZD1839), a reversible growth inhibitor of EGFR-expressing tumour cells, has been shown to enhance the antitumour effect of ionising radiation, and also to increase the uptake of radioiodinated EGF. Thus, combination of gefitinib treatment and radionuclide targeting is an interesting option for therapy of brain tumours that are difficult to treat with conventional methods. The aim of this study was to evaluate how pre-treatment with gefitinib affects binding of astatinated EGF (211At-EGF) to cultured glioma U343 cells, which express high levels of EGFR. The growth of U343 cells in the presence of gefitinib was investigated, and it was found that gefitinib does not significantly inhibit the growth of these cells. Nevertheless, the uptake of 211At-EGF in U343 cells was markedly increased (up to 3.5 times) in cells pre-treated with gefitinib (1 μM). This indicates that a combination of gefitinib treatment and radionuclide targeting to EGFR might be a useful therapeutic modality, even for patients who do not respond to treatment with gefitinib alone. (orig.)

  13. miR-199a-3p targets CD44 and reduces proliferation of CD44 positive hepatocellular carcinoma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Henry, Jon C. [Department of Surgery, Ohio State University Medical Center, Columbus, OH (United States); Park, Jong-Kook; Jiang, Jinmai; Kim, Ji Hye [College of Pharmacy, Ohio State University, Columbus, OH (United States); Nagorney, David M.; Roberts, Lewis R. [Divisions of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN (United States); Banerjee, Soma [Center for Liver Research, School of Digestive and Liver Diseases, Institute of Post Graduate Medical Education and Research, Kolkata (India); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH (United States)

    2010-12-03

    Research highlights: {yields} miR-199a-3p targets CD44 in HCC. {yields} Proliferation and invasion are reduced by miR-199a-3p in CD44+ HCC. {yields} miR-199a-3p is reduced and CD44 protein is increased in HCC tissues. {yields} The duplex form of miR-199a-3p mimetic is required for activity. -- Abstract: Previous work by us and others reported decreased expression of miR-199a-3p in hepatocellular carcinoma (HCC) tissues compared to adjacent benign tissue. We report here a significant reduction of miR-199a-3p expression in 7 HCC cell lines. To determine if miR-199a-3p has a tumor suppressive role, pre-miR-199a-3p oligonucleotides were transfected into the HCC cell lines. Pre-miR-199a-3p oligonucleotide reduced cell proliferation by approximately 60% compared to control oligonucleotide in only two cell lines (SNU449 and SNU423); the proliferation of the other 5 treated cell lines was similar to control oligonucleotide. A pre-miR-199a-3p oligonucleotide formulated with chemical modifications to enhance stability while preserving processing, reduced cell proliferation in SNU449 and SNU423 to the same extent as the commercially available pre-miR-199a-3p oligonucleotide. Furthermore, only the duplex miR-199a-3p oligonucleotide, and not the guide strand alone, was effective at reducing cell viability. Since a CD44 variant was essential for c-Met signaling [V. Orian-Rousseau, L. Chen, J.P. Sleeman, P. Herrlich, H. Ponta, CD44 is required for two consecutive steps in HGF/c-Met signaling, Genes Dev. 16 (2002) 3074-3086] and c-Met is a known miR-199a-3p target, we hypothesized that miR-199a-3p may also target CD44. Immunoblotting confirmed that only the two HCC lines that were sensitive to the effects of pre-miR-199a-3p were CD44+. Direct targeting of CD44 by miR-199a-3p was confirmed using luciferase reporter assays and immunoblotting. Transfection of miR-199a-3p into SNU449 cells reduced in vitro invasion and sensitized the cells to doxorubicin; both effects were enhanced

  14. $^{111m}$Cd- and $^{199m}$Hg-derivatives of blue oxidases

    CERN Multimedia

    2002-01-01

    The rack-induced bonding concept (H.B.Gray & B.G.~Malmstroem, Comments Inorg. Chem, 2, 203, 1983) postulates that the bound metal ion in metalloproteins is forced to adopt a coordination geometry determined by the rigid peptide conformation of the protein. Alternatively, the metal ion could create its own favoured coordination geometry in a soft peptide conformation. In order to decide who is slave or master the changes of coordination and rigidity of metal sites in blue copper proteins due to metal and ligand exchange were studied by $^{111m}$Cd and $^{199m}$Hg $\\gamma$-$\\gamma$-perturbed angular correlation (PAC). To get a better understanding of the so called " Type 1 Copper Site " of the blue oxidases laccase (LAC) and ascorbate oxidase (AO) we concentrated our investigations on the small blue copper proteins azurin and plastocyanin. \\\\ \\\\In azurin~(Az), the metal ligand methionine 121~(M121) was replaced by several amino acids, e.g. asparagine~(N), glutamic acid~(E), via site directed mutagenesis. Di...

  15. Discovery of bla(OXA-199, a chromosome-based bla(OXA-48-like variant, in Shewanella xiamenensis.

    Directory of Open Access Journals (Sweden)

    Zhiyong Zong

    Full Text Available INTRODUCTION: bla(OXA-48 is a globally emerging carbapenemase-encoding gene. The progenitor of bla(OXA-48 appears to be a Shewanella species. The presence of the bla(OXA-48-like gene was investigated for two Shewanella xiamenensis strains. METHODS: Strain WCJ25 was recovered from post-surgical abdominal drainages, while S4 was the type strain of S. xiamenensis. Species identification for WCJ25 was established by sequencing the 16S rDNA and gyrB genes. PCR was used to screen the bla(OXA-48-like genes and to obtain their complete sequences. A phylogenetic tree of the bla(OXA-48-like genes was constructed. The genetic context of the bla(OXA-48-like gene in strain WCJ25 was investigated by inverse PCR using self-ligated AseI- or RsaI-restricted WCJ25 DNA fragments as template, while that in strain S4 was determined by PCR mapping using that in WCJ25 as template. RESULTS: A new bla(OXA-48 variant, designated bla(OXA-48b, with four silent nucleotide differences from the bla(OXA-48 (designated bla(OXA-48a found in the Enterobacteriaceae was identified in strain S4. Strain WCJ25 had a new bla(OXA-48-like variant, bla(OXA-199, with five nucleotide differences from bla(OXA-48a and bla(OXA-48b. The OXA-199 protein has three amino acid substitutions (H37Y, V44A and D153G compared with OXA-48. Both bla(OXA-48b and bla(OXA-199 were found adjacent to genes encoding a peptidase (indicated as orf, a protein of unknown function (sprT, an endonuclease I (endA, and a ribosomal RNA methyl transferase (rsmE upstream and to transcriptional regulator gene lysR and an acetyl-CoA carboxylase-encoding gene downstream. In addition, the insertion sequence ISShes2 was found inserted downstream of bla(OXA-199 but not of bla(OXA-48b. The 26 bp sequences upstream and 63 bp downstream of bla(OXA-48a, bla(OXA-48b and bla(OXA-199 were identical. CONCLUSIONS: bla(OXA-48a, bla(OXA-48b and bla(OXA-199 might have a common origin, suggesting that the bla(OXA-48a gene found in the

  16. Decline in CA19-9 during chemotherapy predicts survival in four independent cohorts of patients with inoperable bile duct cancer

    DEFF Research Database (Denmark)

    Grunnet, Mie; Christensen, Ib J; Lassen, Ulrik;

    2015-01-01

    BACKGROUND: Carbohydrate associated antigen (CA19-9) has been approved by the FDA as a biomarker for monitoring treatment effect in pancreatic cancer. However, the value of serum CA19-9 as a biomarker of response to chemotherapy in bile duct cancer is unclear. The aim of this study was to determi...... confirmed in all three validation cohorts. CONCLUSIONS: CA19-9 response is a robust predictor of survival in patients with inoperable bile duct cancer in four independent data sets....... if a decline in CA19-9 (CA19-9 response) during chemotherapy is predictive of survival in patients with inoperable bile duct cancer. METHODS: Consecutive patients with inoperable bile duct cancer treated at a University Hospital were retrospectively included in an investigational cohort (n = 212......). Three validation cohorts were established including patients 1) participating in phase I/II trials at a Danish Hospital (n = 71), 2) identified retrospectively in a Canadian cohort (n = 196) and 3) randomized in the ABC-02 trial (n = 410). Patients with a baseline CA19-9 and at least one CA19-9 value...

  17. Study on the diagnostic value of combined determination of serum CA125, CA199 and SIL-2R levels in patients with endometriosis

    International Nuclear Information System (INIS)

    Objective: To investigate the diagnostic values of combined determination of serum CA125, CA199 and SIL-2R levels in patients with endometriosis. Methods: Serum CA125, CA199 were measured with RIA and SIL-2R levels with ELISA in 54 patients with endometriosis and 35 controls. Results: The serum levels of CA125, CA199 and SIL-2R in patients with endometriosis were significantly higher than those in controls (P<0.01). The sensitivity and speciality of CA125 for endometriosis was 70.2% and 80.4% respectively, the sensitivity and speciality of CA199 for endometriosis was 62.4% and 71.8% respectively, the sensitivity and speciality of SIL-2R was 89.5% and 60.2% respectively. The sensitivity of the combined determination of CA125, CA199 and SIL-2R for endometriosis was 86.8% being significantly higher than that of CA125 and CA199 respectively. Conclusion: Combined determination of the serum CA125, CA199 and SIL-2R levels in serum can increase the diagnostic sensitivity for endometriosis. (authors)

  18. Clinical Significance and Prognostic Value of CA72-4 Compared with CEA and CA19-9 in Patients with Gastric Cancer

    Directory of Open Access Journals (Sweden)

    M. Ychou

    2000-01-01

    Full Text Available Carcinoembryonic antigen (CEA and CA 19-9 are both widely used in the follow up of patients with gastrointestinal cancer. More recently another tumor marker, named CA 72-4 has been identified and characterized using two different monoclonal antibodies B72.3 and CC49. Several reports evaluated CA 72-4 as a serum tumor marker for gastric cancer and compared its clinical utility with that of CEA or CA 19-9; few reports concerned its prognostic value. In the present study, CA 72-4 is evaluated and compared with CEA and CA 19-9 in various populations of patients with gastric cancer and benign disease; for 52 patients with gastric adenocarcinoma and 57 patients without neoplastic disease CEA, CA 19-9 and CA 72-4 were evaluated before treatment. Sensitivity of the tumor markers CA 72-4, CA 19-9 and CEA at the recommended cut-off level in all 52 patients were 58%, 50% the sensitivity increased to 75%. of these markers, for non metastatic patients, multivariate analyses indicated that none of the markers were significant, when adjusted for gender and age (which were indicators of poor prognosis; patients with abnormal values of CA72-4 tended to have shorter survival than patients with normal values (p < 0.07. In the metastatic population, only high values of CA19-9 (p < 0.02 and gender (women (p < 0.03 were indicators of poor prognosis in univariate analysis; multivariate analysis revealed that both CA72-4 (p = 0.034 and CA19-9 p = 0.009, adjusted for gender were independent prognostic factors. However, CA72-4 lost significance (p = 0.41 when adjusted for CA19-9 and gender, indicating that CA19-9 provides more prognostic information than CA72-4.

  19. Residues R199H200 of prototype foamy virus transactivator Bel1 contribute to its binding with LTR and IP promoters but not its nuclear localization

    International Nuclear Information System (INIS)

    Prototype foamy virus encodes a transactivator called Bel1 that enhances viral gene transcription and is essential for PFV replication. Nuclear localization of Bel1 has been reported to rely on two proximal basic motifs R199H200 and R221R222R223 that likely function together as a bipartite nuclear localization signal. In this study, we report that mutating R221R222R223, but not R199H200, relocates Bel1 from the nucleus to the cytoplasm, suggesting an essential role for R221R222R223 in the nuclear localization of Bel1. Although not affecting the nuclear localization of Bel1, mutating R199H200 disables Bel1 from transactivating PFV promoters. Results of EMSA reveal that the R199H200 residues are vital for the binding of Bel1 to viral promoter DNA. Moreover, mutating R199H200 in Bel1 impairs PFV replication to a much greater extent than mutating R221R222R223. Collectively, our findings suggest that R199H200 directly participate in Bel1 binding to viral promoter DNA and are indispensible for Bel1 transactivation activity. - Highlights: • The R221R222R223 residues are essential for the nuclear localization of Bel1. • Although not affecting the nuclear localization of Bel1, mutating R199H200 disables Bel1 from transactivating PFV promoters. • The R199H200 residues directly participate in Bel1 binding to viral promoter DNA. • Mutating R199H200 in Bel1 impairs PFV replication to a much greater extent than mutating R221R222R223

  20. 199mHg-derivatives of ascorbate oxidase and laccase: selective depletion and blocking of Cu-sites

    International Nuclear Information System (INIS)

    We report on the 199mHg nuclear quadrupole interaction (NQI) of Hg-derivatives of the blue oxidases ascorbate oxidase (AO) and laccase (LAC). For fully reconstituted enzymes, three different NQIs were observed. The assignment of these NQIs to the type-1, -2, and -3 Cu-sites is based on type-2 depleted AO, on blocking studies with inactive Hg prior to 199mHg/carrier reconstitution, and on the population ratio observed for fully reconstituted LAC. The NQIs for both enzymes are similar, suggesting similar Cu-sites. The type-2 site is preferentially reconstituted, contrary to expectations. Neither the blocking nor the depletion is as selective as expected. (orig.)

  1. Comparison of the Larmor precession frequencies of 199Hg and ultracold neutrons in the nEDM experiment at PSI

    International Nuclear Information System (INIS)

    Full text: The neutron electric dipole moment (nEDM) experiment at the Paul Scherrer Institut (PSI) measures the Larmor precession frequencies of ultracold neutrons (UCN) in parallel and antiparallel magnetic and electric fields. Nuclear spin polarized 199Hg atoms are utilized as cohabiting magnetometer and monitor changes of the magnetic field within the same volume as the UCN. Due to the different velocities of the two species and the effect of gravity, the magnetic field of the precession volume is sampled differently by UCN (∼4 m/s) and 199Hg ((∼170 m/s), respectively. Thus, in presence of vertical magnetic field gradients, the two species will systematically measure different fields. I will present measurements of the ratio of the two precession frequencies as function of vertical gradients performed with the nEDM apparatus in 2012. This quantity is an important tool to investigate systematic effects and also physical properties of the involved species. (author)

  2. Hg-coordination studies of oligopeptides containing cysteine, histidine and tyrosine by $^{199m}$Hg-TDPAC

    CERN Document Server

    Ctortecka, B; Mallion, S; Butz, T; Hoffmann, R

    1999-01-01

    In order to study the interaction of histidine- and tyrosine- containing peptide chains with Hg(II), the nuclear quadrupole interaction (NQI) of /sup 199m/Hg in the Hg complexes of the oligopeptides alanyl-alanyl-histidyl-alanyl-alanine-amid (AAHAA-NH /sub 2/) and alanyl-alanyl-tyrosyl-alanyl-alanine-amid (AAYAA-NH/sub 2/) was determined by time differential perturbed angular correlation and is compared with previous data on alanyl-alanyl-cysteyl-alanyl- alanyl (AACAA-OH). The /sup 199m/Hg-NQIs depend on the oligopeptide to Hg(II) stoichiometry and indicate that two-fold and four-fold coordinations occur for the bound Hg(II). (12 refs).

  3. Systematic errors in the measurement of the permanent electric dipole moment (EDM) of the 199 Hg atom

    Science.gov (United States)

    Chen, Yi; Graner, Brent; Heckel, Blayne; Lindahl, Eric

    2016-05-01

    This talk provides a discussion of the systematic errors that were encountered in the 199 Hg experiment described earlier in this session. The dominant systematic error, unseen in previous 199 Hg EDM experiments, arose from small motions of the Hg vapor cells due to forces exerted by the applied electric field. Methods used to understand this effect, as well as the anticipated sources of systematic errors such as leakage currents, parameter correlations, and E2 and v × E / c effects, will be presented. The total systematic error was found to be 72% as large as the statistical error of the EDM measurement. This work was supported by NSF Grant 1306743 and by DOE Grant DE-FG02-97ER41020.

  4. Identification of a Novel Human Papillomavirus, Type HPV199, Isolated from a Nasopharynx and Anal Canal, and Complete Genomic Characterization of Papillomavirus Species Gamma-12.

    Science.gov (United States)

    Oštrbenk, Anja; Kocjan, Boštjan J; Hošnjak, Lea; Li, Jingjing; Deng, Qiuju; Šterbenc, Anja; Poljak, Mario

    2015-01-01

    The novel human papillomavirus type 199 (HPV199) was initially identified in a nasopharyngeal swab sample obtained from a 25 year-old immunocompetent male. The complete genome of HPV199 is 7,184 bp in length with a GC content of 36.5%. Comparative genomic characterization of HPV199 and its closest relatives showed the classical genomic organization of Gammapapillomaviruses (Gamma-PVs). HPV199 has seven major open reading frames (ORFs), encoding five early (E1, E2, E4, E6, and E7) and two late (L1 and L2) proteins, while lacking the E5 ORF. The long control region (LCR) of 513 bp is located between the L1 and E6 ORFs. Phylogenetic analysis additionally confirmed that HPV-199 clusters into the Gamma-PV genus, species Gamma-12, additionally containing HPV127, HV132, HPV148, HPV165, and three putative HPV types: KC5, CG2 and CG3. HPV199 is most closely related to HPV127 (nucleotide identity 77%). The complete viral genome sequence of additional HPV199 isolate was determined from anal canal swab sample. Two HPV199 complete viral sequences exhibit 99.4% nucleotide identity. To the best of our knowledge, this is the first member of Gamma-PV with complete nucleotide sequences determined from two independent clinical samples. To evaluate the tissue tropism of the novel HPV type, 916 clinical samples were tested using HPV199 type-specific real-time PCR: HPV199 was detected in 2/76 tissue samples of histologically confirmed common warts, 2/108 samples of eyebrow hair follicles, 2/137 anal canal swabs obtained from individuals with clinically evident anal pathology, 4/184 nasopharyngeal swabs and 3/411 cervical swabs obtained from women with normal cervical cytology. Although HPV199 was found in 1.4% of cutaneous and mucosal samples only, it exhibits dual tissue tropism. According to the results of our study and literature data, dual tropism of all Gamma-12 members is highly possible. PMID:26375679

  5. Measurement of a false electric dipole moment signal from $^{199}$Hg atoms exposed to an inhomogeneous magnetic field

    OpenAIRE

    Afach, S.; Baker, C.A.; Ban, G.; Bison, G.; K. Bodek; Chowdhuri, Z.; Daum, M.; Fertl, M.; Franke, B.; Geltenbort, P.(Institut Laue-Langevin, Grenoble Cedex 9, 38042, France); Green, K.; van der Grinten, M. G. D.; Grujic, Z.; Harris, P.G.; Heil, W.

    2015-01-01

    We report on the measurement of a Larmor frequency shift proportional to the electric-field strength for $^{199}{\\rm Hg}$ atoms contained in a volume permeated with aligned magnetic and electric fields. This shift arises from the interplay between the inevitable magnetic field gradients and the motional magnetic field. The proportionality to electric-field strength makes it apparently similar to an electric dipole moment (EDM) signal, although unlike an EDM this effect is P- and T-conserving....

  6. CA 19-9 Level as Indicator of Early Distant Metastasis and Therapeutic Selection in Resected Pancreatic Cancer

    International Nuclear Information System (INIS)

    Purpose: In patients with pancreatic cancer treated with curative resection, we evaluated the effect of clinicopathologic parameters on early distant metastasis within 6 months (DM6m) to identify patients who might benefit from surgery. Methods and Materials: The study involved 84 patients with pancreatic cancer who had undergone curative resection between August 2001 and April 2009. The parameters of gender, age, tumor size, histologic differentiation, T classification, N classification, pre- and postoperative carbohydrate antigen (CA) 19-9 level, resection margin, and adjuvant chemoradiotherapy were analyzed to identify the risk factors associated with DM6m. Results: Of the 84 patients, locoregional recurrence developed in 35 (41.7%) and distant metastasis in 58 (69%). Of the 58 patients with distant metastasis, DM6m had developed in 27 (46.6%). Multivariate analysis showed that preoperative CA 19-9 level was significantly associated with DM6m (p 6m was observed in 9.1%, 50%, and 80% of those with a preoperative CA 19-9 level of ≤100 U/mL, 101–400 U/mL, and >400 U/mL, respectively (p 6m and to identify those who would benefit from surgical resection.

  7. Diagnostic value of combined measurement of serum CEA, AFP, CA19-9 and SA levels in patients with primary hepatic cancer

    International Nuclear Information System (INIS)

    Objective: To study the diagnostic value of combined measurement of serum CEA, AFP, CA19-9 and SA levels for primary hepatic cancer. Methods: Serum CEA, AFP, CA19-9 (with RIA) SA (with biochemistry) levels were measured in 36 patients with primary hepatic cancer and 35 controls. Results: As a single tumor marker for diagnosis, the sensitivity of CEA, AFP, CA19-9, SA was 33.3%, 69.4%, 58.5% and 61.1% respectively, the specificity was 60.4%, 81.3% 68.8%, 64.4% respectively. With combined detection of the four markers and assuming two or more markers positive as positive, the sensitivity would be 94.4% and specificity would be 95.8%. Conclusion: Combined measurement of serum CEA, AFP, CA19-9 and SA levels is of clinical usefulness for diagnosis of primary hepatic cancer. (authors)

  8. CA19-9-related tumor kinetics after first-line chemotherapy of patients with advanced pancreatic cancer: a monoinstitutional experience.

    Science.gov (United States)

    Colloca, Giuseppe; Venturino, Antonella; Guarneri, Domenico

    2016-09-01

    The absolute value of carbohydrate antigen 19-9 (CA19-9) pretreatment and its reduction after chemotherapy are established prognostic variables for patients with advanced pancreatic cancer. The present study is a retrospective monoinstitutional evaluation of the prognostic role of the CA19-9 reduction and some CA19-9-related tumor kinetics parameters, such as tumor growth rate constant (G), kinetic tumor response and log ratio. Forty-one cases met the selection criteria. After 8 weeks only G reported an inverse relationship with OS (r = -0.494) that was confirmed by regression analysis (R (2) = 0.192). G after 8 weeks of chemotherapy appears as a possible surrogate end point of overall survival. PMID:27522503

  9. Delayed radiation-induced inflammation accompanying a marked carbohydrate antigen 19-9 elevation in a patient with resected pancreatic cancer

    Science.gov (United States)

    Mattes, Malcolm D.; Cardinal, Jon S.; Jacobson, Geraldine M.

    2016-01-01

    Although carbohydrate antigen (CA) 19-9 is a useful tumor marker for pancreatic cancer, it can also become elevated from a variety of benign and malignant conditions. Herein we describe an unusual presentation of elevated CA 19-9 in an asymptomatic patient who had previously undergone adjuvant chemotherapy and radiation therapy for resected early stage pancreatic cancer. The rise in CA 19-9 might be due to delayed radiation-induced inflammation related to previous intra-abdominal radiation therapy with or without radiation recall induced by gemcitabine. After treatment with corticosteroids the CA 19-9 level decreased to normal, and the patient has not developed any evidence of recurrent cancer to date. PMID:27306770

  10. Cisplatin-induced downregulation of miR-199a-5p increases drug resistance by activating autophagy in HCC cell

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Ning; Zhang, Jianjun; Shen, Conghuan; Luo, Yi; Xia, Lei; Xue, Feng [Department of Transplantation and Hepatic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, 1630 Dongfang Road, Shanghai 200127, People' s Republic of China (China); Xia, Qiang, E-mail: xiaqiang1@yahoo.com.cn [Department of Transplantation and Hepatic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, 1630 Dongfang Road, Shanghai 200127, People' s Republic of China (China)

    2012-07-13

    Highlights: Black-Right-Pointing-Pointer miR-199a-5p levels were significantly decreased after cisplatin treatment. Black-Right-Pointing-Pointer Cisplatin treatment induced autophagy activation. Black-Right-Pointing-Pointer Cisplatin-induced downregulation of miR-199a-5p increases drug resistance by activating autophagy in HCC cell. -- Abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Systemic chemotherapy plays an important role in the treatment of patients with advanced liver cancer. However, chemoresistance to cisplatin is a major limitation of cisplatin-based chemotherapy in the clinic, and the underlying mechanism of such resistance is not fully understood. In the study, we found that miR-199a-5p levels were significantly reduced in HCC patients treated with cisplatin-based chemotherapy. Cisplatin treatment also resulted in decreased miR-199a-5p levels in human HCC cell lines. Forced expression of miR-199a-5p promoted cisplatin-induced inhibition of cell proliferation. Cisplatin treatment activated autophagy in Huh7 and HepG2 cells, which increased cell proliferation. We further demonstrated that downregulated miR-199a-5p enhanced autophagy activation by targeting autophagy-associated gene 7 (ATG7). More important, autophagy inhibition abrogated miR-199a-5p downregulation-induced cell proliferation. These data demonstrated that miR-199a-5p/autophagy signaling represents a novel pathway regulating chemoresistance, thus offering a new target for chemotherapy of HCC.

  11. Diagnostic value of combined determinations of serum CA19-9, CA242 and CA50 levels in patients with pancreatic cancer

    International Nuclear Information System (INIS)

    Objective: To explore the diagnostic value of combined determinations of serum CA19-9, CA242 and CA50 levels in patients with pancreatic cancer. Methods: Serum CA19-9, CA242 and CA50 levels were determined with RIA in 120 patients with pancreatic cancer, 50 patients with chronic pancreatitis and 80 controls. Results: The serum levels of CA19-9, CA242 and CA50 in patients with pancreatic cancer were significantly higher than those in patients with pancreatitis and controls. With CA19-9, the diagnostic sensitivity and specificity for pancreatic cnrcinoma was 82.0% and 78.6% respectively. With CA242, the diagnostic sensitivity and specificity for pancreatic carcinoma was 78.5% and 81.6% respectively. With CA50, the diagnostic sensitivity and specificity for pancreatic carcinoma was 52.7% and 74.6% respectively. With combined determinations of CA19-9, CA242 and CA50, the sensitivity and specificity was enhanced to 96.82% and 98.75% respectively. Conclusion: Combined detection of CA19-9, CA242 and CA50 is clinically useful for early diagnosis of pancreatic cancer. (authors)

  12. Comparison of effectiveness of whole viral,N and N199 proteins by ELISA for the rapid diagnosis of severe acute respiratory syndrome coronavirus

    Institute of Scientific and Technical Information of China (English)

    GUO Zhong-min; ZHONG Nan-shan; ZHU Xing-quan; LU Jia-hai; HAN Wen-yu; LIU Ze-yu; LI Guo-wei; LIAO Jia-wei; WANG Shu-min; WU Ying-song; ZHENG Huan-ying

    2007-01-01

    Background Although severe acute respiratory syndrome(SARS)has been controlled,the subsequently emerging sporadic cases in 2004 emphasize the necessity of developing a rapid diagnostic method,which would be of great help in clinical diagosis and also wild host screening.This study aims to establish an effective and rapid serological tool for the diagnosis of SARS-CoV by comparison among whole viral,N and N199 proteins by ELISA.Methods SARS-CoV N and N199(a truncated nucleocapsid gene)genes were cloned,expressed,identified by Western blotting,and applied in screening of human and swine samples.Sera of SARS convalescent-phase patients,normal human sera,sera of patients with other respiratory diseases,and swine sera were screened by ELISA,with whole SARS-CoV F69,N and N199 proteins as antigens.Results The sensitivity and specificity of N and N199 proteins in human sera diagnosis were approximate(P=0.743),which was higher than whole viral protein but the difference was not significant(P=0.234).The N199 protein proved to be more specific in swine sera screening than whole viral and N protein(P<0.001).Conclusion N199 protein is feasible in both clinical diagnosis and SARS-CoV reservoir screening.

  13. Correlation and Astronomical Calibration of Pacific Sediments From ODP Leg 199

    Science.gov (United States)

    Pälike, H.; Moore, T. C.; Janecek, T. R.

    2002-12-01

    One of the great successes of Leg 199 was the recovery of a high-resolution (~ 1-2 cm / ky) biogenic sediment record from the late Paleocene to the early Miocene. These sediments were found to contain an uninterrupted set of geomagnetic chrons, as well as a detailed record of calcareous and siliceous biostratigraphic datum points. In addition, lithological measurements revealed clearly recognisable cycles that can be attributed to climatic change, driven by Milankovitch style orbital variations of the Earth. Discovering drill sites with a well-defined magneto- and biostratigraphic record that also show clear lithological cycles is rare and valuable, and opens the opportunity to develop a detailed stratigraphic inter-site correlation, as well as providing the data to refine and extend the astronomical time calibration for parts of the Cenozoic. The basis for stratigraphic correlation and time scale calibration is a complete and representative sedimentary record with a high signal-to-noise ratio in the lithological data. Shipboard work allowed the generation of a continuous "spliced" record, formed by correlation of at least two holes drilled at the same site. However, differential stretching and squeezing of sedimentary features, due to both coring and geological processes, result in events that are not aligned in the depth domain. We present the results of extensive post-cruise work that resulted in the generation of a revised composite depth stack that puts data from all holes of sites 1218 and 1219 into a common depth framework. It was possible to extrapolate magneto- and biostratigraphic datum points between these two sites (separated by ~ 750 km). This procedure allowed the generation of a "site composite record", which provides smaller uncertainty intervals for bio- and magnetostratigraphic zones, as well as giving refined and more detailed preliminary age models for either site. We then use the aligned and stacked lithological data from sites 1218 and 1219 to

  14. Five Year Results of US Intergroup/RTOG 9704 With Postoperative CA 19-9 {<=}90 U/mL and Comparison to the CONKO-001 Trial

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Adam C., E-mail: adam.berger@jefferson.edu [Thomas Jefferson University Hospital, Philadelphia, Pennsylvania (United States); Winter, Kathryn [RTOG Statistical Center, Philadelphia, Pennsylvania (United States); Hoffman, John P. [Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Regine, William F. [University of Maryland Medical Systems, Baltimore, Maryland (United States); Abrams, Ross A. [Rush University Medical Center, Chicago, Illinois (United States); Safran, Howard [Division of Hematology/Oncology, The Miriam Hospital, Providence, Rhode Island (United States); Freedman, Gary M. [Fox Chase Cancer Center, Philadelphia, Pennsylvania (United States); Benson, Alan B. [Northwestern Memorial Hospital, Chicago, Illinois (United States); MacDonald, John [St. Vincent' s Comprehensive Cancer Center, New York, New York (United States); Willett, Christopher G. [Duke University Medical Center, Durham, North Carolina (United States)

    2012-11-01

    Purpose: Radiation Therapy Oncology Group (RTOG) trial 9704 was the largest randomized trial to use adjuvant chemoradiation therapy for patients with pancreatic cancer. This report analyzes 5-year survival by serum level of tumor marker CA 19-9 of {<=}90 vs >90 U/mL and compares results to the those of the CONKO-001 trial. Methods and Materials: CA 19-9 expression was analyzed as a dichotomized variable ({<=}90 vs >90 U/mL). Cox proportional hazard models were used to identify the impact of the CA 19-9 value on overall survival (OS). Actuarial estimates of OS were calculated using the Kaplan-Meier method. Results: Both univariate (hazard ratio [HR] = 3.2; 95% confidence interval [CI], 2.3-4.3, P<.0001) and multivariate (HR = 3.1; 95% CI, 2.2-4.2, P<.0001) analyses demonstrated a statistically significant decrease in OS for CA 19-9 serum level of {>=}90 U/mL. For patients in the gemcitabine (Gem) treatment arm with CA 19-9 <90 U/mL, median survival was 21 months. For patients with CA 19-9 {>=}90 U/mL, this number dropped to 10 months. In patients with pancreatic head tumors in the Gem treatment arm with RT quality assurance per protocol and CA 19-9 of <90 U/mL, median survival and 5-year rate were 24 months and 34%. In comparison, the median survival and 5-year OS rate for patients in the Gem arm of the CONKO trial were 22 months and 21%. Conclusions: This analysis demonstrates that patients with postresection CA 19-9 values {>=}90 U/mL had a significantly worse survival. Patients with pancreatic head tumors treated with Gem with CA 19-9 serum level of <90 U/mL and per protocol RT had favorable survival compared to that seen in the CONKO trial. CA 19-9 is a stratification factor for the current RTOG adjuvant pancreas trial (0848).

  15. Effect of Biofield Treatment on Physical, Thermal, and Spectral Properties of SFRE 199-1 Mammalian Cell Culture Medium

    OpenAIRE

    Trivedi, Mahendra Kumar

    2015-01-01

    SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently, the control and trea...

  16. ITRAQ-based quantitative proteomics reveals apolipoprotein A-I and transferrin as potential serum markers in CA19-9 negative pancreatic ductal adenocarcinoma

    Science.gov (United States)

    Lin, Chao; Wu, Wen-Chuan; Zhao, Guo-Chao; Wang, Dan-Song; Lou, Wen-Hui; Jin, Da-Yong

    2016-01-01

    Abstract Currently the diagnosis of pancreatic ductal adenocarcinoma (PDAC) relies on CA19-9 and radiological means, whereas some patients do not have elevated levels of CA19-9 secondary to pancreatic cancer. The purpose of this study was to identify potential serum biomarkers for CA19-9 negative PDAC. A total of 114 serum samples were collected from 3 groups: CA19-9 negative PDAC patients (n = 34), CA19-9 positive PDAC patients (n = 44), and healthy volunteers (n = 36), whereas the first 12 samples from each group were used for isobaric tags for relative and absolute quantitation (iTRAQ) analysis. Thereafter, candidate biomarkers were selected for validation by enzyme-linked immunosorbent assay (ELISA) with the rest specimens. Using the iTRAQ approach, a total of 5 proteins were identified as significantly different between CA19-9 negative PDAC patients and healthy subjects according to our defined criteria. Apolipoprotein A-I (APOA-I) and transferrin (TF) were selected to validate the proteomic results by ELISA in a further 78 serum specimens. It revealed that TF significantly correlated with the degree of histological differentiation (P = 0.042), and univariate and multivariate analyses indicated that TF is an independent prognostic factor for survival (hazard ratio, 0.302; 95% confidence interval, 0.118–0.774; P = 0.013) of patients with PDAC after curative surgery. ITRAQ-based quantitative proteomics revealed that APOA-I and TF may be potential CA19-9 negative PDAC serum markers. PMID:27495108

  17. Targeting of multiple myeloma-related angiogenesis by miR-199a-5p mimics: in vitro and in vivo anti-tumor activity.

    Science.gov (United States)

    Raimondi, Lavinia; Amodio, Nicola; Di Martino, Maria Teresa; Altomare, Emanuela; Leotta, Marzia; Caracciolo, Daniele; Gullà, Annamaria; Neri, Antonino; Taverna, Simona; D'Aquila, Patrizia; Alessandro, Riccardo; Giordano, Antonio; Tagliaferri, Pierosandro; Tassone, Pierfrancesco

    2014-05-30

    Multiple myeloma (MM) cells induce relevant angiogenic effects within the human bone marrow milieu (huBMM) by the aberrant expression of angiogenic factors. Hypoxia triggers angiogenic events within the huBMM and the transcription factor hypoxia-inducible factor-1α (HIF-1α) is over-expressed by MM cells. Since synthetic miR-199a-5p mimics negatively regulates HIF-1α, we here investigated a miRNA-based therapeutic strategy against hypoxic MM cells. We indeed found that enforced expression of miR-199a-5p led to down-modulated expression of HIF-1α as well as of other pro-angiogenic factors such as VEGF-A, IL-8, and FGFb in hypoxic MM cells in vitro. Moreover, miR-199a-5p negatively affected MM cells migration, while it increased the adhesion of MM cells to bone marrow stromal cells (BMSCs) in hypoxic conditions. Furthermore, transfection of MM cells with miR-199a-5p significantly impaired also endothelial cells migration and down-regulated the expression of endothelial adhesion molecules such as VCAM-1 and ICAM-1. Finally, we identified a hypoxia\\AKT/miR-199a-5p loop as a potential molecular mechanism responsible of miR-199a-5p down-regulation in hypoxic MM cells. Taken together our results indicate that miR-199a-5p has an important role for the pathogenesis of MM and support the hypothesis that targeting angiogenesis via a miRNA/HIF-1α pathway may represent a novel potential therapeutical approach for this still lethal disease. PMID:24839982

  18. Effect of ultrasound combined with determination of CA19-9 and hs-CRP on the accuracy of early diagnosis of gallbladder cancer

    Directory of Open Access Journals (Sweden)

    Hang-yu ZHANG

    2014-03-01

    Full Text Available Objective  To investigate the value of ultrasound combined with tumor marker CA19-9 and high sensitivity C reactive protein (hs-CRP on the accuracy of early diagnosis of gallbladder cancer. Methods  Ninety-five patients with pathologically confirmed primary gallbladder cancer who received cholecystectomy from Jan. 2008 to Jan. 2013 in our hospital were included in the study. The study also included 51 patients with benign cystic lesions and 51 healthy adults. In all patients, results of pathological examination, B-ultrasound, serum CA19-9 and hs-CRP detection were obtained. In all the healthy adults gallbladder diseases were ruled out, but all of them received serum CA19-9 and hs-CRP determination. Results  Of 95 patients with primary gallbladder cancer, B-ultrasound showed nodules in the gallbladder lumen in 31, tumor mass in 40, and thickened wall in 24. Histological examination showed adenocarcinoma in 87 cases, adenosquamous carcinoma in 6, and squamous carcinoma in 2. There was a statistical difference in hs-CRP and CA19-9 levels between the groups (P<0.05, and the highest level appeared in gallbladder cancer group. There was a high sensitivity but low specificity of CA19-9 or hs-CRP in the diagnosis of primary gallbladder cancer. The sensitivity and specificity were 74.7% and 75.0% when primary gallbladder cancer was diagnosed with B-ultrasound. The positive values of CA19-9 and hs-CRP were ≥100U/ml and ≥6mg/L respectively, when they were combined with B-ultrasound as a single serology factor. When both B-ultrasound and serum levels of CA19-9 and hs-CRP were positive, the diagnostic sensitivity and specificity were 71.6% and 84.4% respectively. When B-ultrasound or serum CA19-9 and hs-CRP were positive, the diagnostic sensitivity and specificity were 98.9% and 66.7% respectively. Conclusions  Combination of B-ultrasound and determination of serum of CA19-9 and hs-CRP can improve the accuracy of the diagnosis of gallbladder

  19. Factors related to serum level of carbohydrate antigen 19-9 and cancer antigen 125 in healthy rural populations in Korea

    International Nuclear Information System (INIS)

    This study examines the levels of carbohydrate antigen 19-9(CA 19-9) and cancer antigen 125(CA125) in serum and its related factors in healthy Korean population. Although CA19-9 and CA125 have been widely used tumor markers for gastroenteric cancers and ovarian cancer in Western countries, there are no information available on the serum levels of CA19-9 and CA125 in healthy population and the factors affecting the levels of these tumor markers in Korea. A cross-sectional study was performed to measure CA19-9 and CA125 among 76 healthy males and 95 healthy females in Korea. CA19-9 and CA125 were quantitated using solid-phase radioimmunoassay kits. Informations on the factors which might be related to the levels of these markers were collected by questionnaire(e.g., smoking, alcohol consumption, menstruation, oral pill use, breast-feeding history, etc.). There was no statistically significant difference in the mean of CA19-9 concentration between men(10.4 u/ml) and women (10.1 u/ml), whereas the mean of CA125 levels (11.2 u/ml) was higher in women than that(2.5 u/ml) in men. Although there was a statistically significant association between CA19-9 and average number of cigarette consumed per day (r=0.59, p=0.026) and total number of cigarettes consumed in women (r=0.74, p=0.003), the significance disappeared by multiple regression analysis after adjusting age and body mass index. Later age of menopause(p=0.035) and longer duration of breast-feeding(p=0.050) were significant predictors for CA125 levels in women by multiple regression analysis after adjusting age and body mass index. In conclusion, CA19-9 can be used as a stable tumor marker in clinical practices, however, menstruation and breast-feeding should be considered when CA125 is used in women

  20. MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway

    Directory of Open Access Journals (Sweden)

    Long Jia

    2013-12-01

    Full Text Available MicroRNAs constitute a class of ~22-nucleotide non-coding RNAs. They modulate gene expression by associating with the 3' untranslated regions (3' UTRs of messenger RNAs (mRNAs. Although multiple miRNAs are known to be regulated during myoblast differentiation, their individual roles in muscle development are still not fully understood. In this study, we showed that miR-199a-3p was highly expressed in skeletal muscle and was induced during C2C12 myoblasts differentiation. We also identified and confirmed several genes of the IGF-1/AKT/mTOR signal pathway, including IGF-1, mTOR, and RPS6KA6, as important cellular targets of miR-199a-3p in myoblasts. Overexpression of miR-199a-3p partially blocked C2C12 myoblast differentiation and the activation of AKT/mTOR signal pathway, while interference of miR-199a-3p by antisense oligonucleotides promoted C2C12 differentiation and myotube hypertrophy. Thus, our studies have established miR-199a-3p as a potential regulator of myogenesis through the suppression of IGF-1/AKT/mTOR signal pathway.

  1. Prepro-thyrotropin releasing hormone 178-199 immunoreactivity is altered in the hypothalamus of the Wistar-Kyoto strain of rat.

    Science.gov (United States)

    Suzuki, S; Solberg, L C; Redei, E E; Handa, R J

    2001-09-21

    The rat prepro-thyrotropin releasing hormone (TRH) 178-199 is derived from prepro-TRH by the actions of the endopeptidases, prohormone convertase 1 (PC1) and PC2. PPTRH 178-199 attenuates the synthesis and secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary both in vitro and in vivo, suggesting an inhibitory action on hypothalamic-pituitary-adrenal (HPA) axis function. This peptide also acts centrally to increase activity and decrease anxiety related behaviors. To elucidate the involvement of this peptide in these functions, we have compared the expression of PPTRH 178-199, PPTRH mRNA, and PC1 and PC2 mRNAs in the Wistar-Kyoto (WKY) and Wistar strains of rat. WKY rats have been shown to possess neuroendocrine abnormalities (HPA hyper-activity) and hyper-emotional behavioral characteristics. Immunohistochemical analysis of PPTRH 178-199 demonstrated significant strain differences in the paraventricular nucleus (PVN) of the hypothalamus and the parastrial nucleus (PSN). WKY rats had significantly greater numbers of immunoreactive (IR) cell body profiles (PHPA axis and the hyper-emotional behavioral characteristics seen in this rat strain. Such data fit with the hypothesis that PPTRH 178-199 is involved in the regulation of the HPA axis and behavior. PMID:11549391

  2. Study on the diagnostic value of determination of changes of serum CA125 and CA19-9 levels in patients with endometriosis

    International Nuclear Information System (INIS)

    Objective: To explore the diagnostic value of determination of changes of serum CA125 and CA19-9 levels in patients with endometriosis. Methods: Serum CA125 and CA19-9 levels were determined with RIA in 45 patients with endometriosis and 40 controls. Results: Serum levels of CA125 and CA19-9 in patients with endometriosis (89.63 ± 30.16u/ml and 95.62±43.67u/ml respectively) were significantly higher than those (16.26±6.37u/ml and 20.26±8.25u/ml respectively) in controls (P<0. 001). Sensitivity of CA125 for diagnosis of endometriosis was 77.78% and that of CA19-9 was 62.22%, while the specificity was 92.50% and 90.00% respectively. If we only took the patients with both markers elevated as being diagnostically positive, the sensitivity would naturally be lower (57.78%) but the specificity would be 100%. Levels of the markers were significantly higher in stage III, IV patients than those in patients with stage I, II disease (P<0.01). Conclusion: Combined determination of serum CA125 and CA19-9 levels were of highly specific diagnostic value in patients with endometriosis. (authors)

  3. 血清肿瘤标志物AFP、CEA和CA199联合检测在消化系统恶性肿瘤诊断中的临床价值%The clinical value of combined detection of serum tumor markers AFP, CEA and CA199 in the diagnosis of digestive system malignancies

    Institute of Scientific and Technical Information of China (English)

    徐惠明; 周明霞; 张群雄; 沈伟春

    2011-01-01

    目的 探讨血清肿瘤标志物甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原199(CA199)联合检测在消化系统恶性肿瘤诊断中的临床价值.方法 回顾性分析203例因消化系统疾病住院的患者,经内镜、病理及腹部CT确诊,其中消化系统恶性肿瘤125例,消化系统良性疾病78例,均应用化学发光法检测血清AFP、CEA和CA199的水平.结果 恶性肿瘤患者AFP、CEA、CA199均高于良性消化疾病患者,差异有统计学意义(P<0.05或P<0.01).AFP、CEA与CA199联合检测较单项检测对消化系统恶性肿瘤诊断的敏感度更高.结论 血清肿瘤标志物AFP、CEA和CA199检测作为诊断性试验,在消化道恶性肿瘤的诊断中具有重要的价值,而联合检测使敏感度得到明显提高,降低了消化道肿瘤的漏诊率.%Objective To evaluate and discuss the clinical value of combined detection of serum tumor makers AFP, CEA and CA199 in several diagnosed malignancies of digestive system.Methods The single and combined detecting results of AFP, CEA, CA199 in 203 patients with digestive tract diseases were retrospectively analyzed. Seventy - eight patients with benign diseases and 125 patients with malignant tumors were diagnosed by endoscopy, pathology and abdominal CT. Results The patients with malignant tumors had higher levels of AFP, CEA and CA199 that of the patients with benign diseases(P<0.05 or P<0.01). The combined detection of AFP, CEA and CA199 made the sensitivity increased. Conclusion Serum tumor markers AFP, CEA and CA199 are of an important value as a diagnostic test for malignant tumors in digestive system. The combined detection can improve the sensitivity and reduce the rate of missed diagnosis of gastrointestinal cancer.

  4. Treatment of Ruptured Ovarian Endometrioma with Extremely High CA 125, Moderately High CA 19-9 and CA 15-3 Level

    Directory of Open Access Journals (Sweden)

    Muzeyyen Duran

    2013-06-01

    Full Text Available In this case report, a ruptured ovarian endometrioma with a very high CA-125 level, moderately elevated CA 19-9, and CA 15-3 levels is presented. A 20 years old patient, complaining from pelvic pain, 5 cm adnexial mass was detected on left side. Biochemical examination was revealed very high CA-125 value (2556IU/ml, moderately elevated CA 19-9 (134IU/ml, and CA 15-3 (65IU/ml values. Laparoscopy was done and a ruptured ovarian endometrioma of 5 cm was seen during operation. After the total excision of the cyst, tumor markers fell rapidly. Very high CA-125 value, moderately elevated CA19-9, and CA 15-3 values can be seen in cases with ruptured endometrioma. In young patients, endometrioma must be considered firstly and laparoscopy should be applied instead of more invasive methods unless there was any finding or strong suspicion about malignancy.

  5. Clinical value of combining tumor maker alphafetoprotein (AFP),CA125 and CA19-9 in diagnose of liver cirrhosis and PHC%血清AFP、CA125、CA19-9联检在肝硬化和肝癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    徐笛; 贾民; 胡兰英

    2009-01-01

    目的:探讨肿瘤标志物甲胎蛋白(AFP)、糖原抗原CA125与CA19-9在肝硬化和原发性肝癌(PHC)中的诊断价值.方法:对原发性肝癌(PHC)组50例、肝硬化组40例、慢性肝炎组45例和正常对照组30例,检测血清AFP、CA125和CA19-9水平.结果:PHC组血清AFP、CA125、CA19-9水平均明显高于肝炎组及正常对照组,差异有统计学意义(P<0.05).PHC组血清AFP、CA125和CA19-9阳性率分别为70.0%、82.0%、90.0%,CA125、AFP联检阳性率为91.2%,AFP、CA125与CA19-9联检阳性率达97.3%.结论:血清AFP、CA125和CA19-9联合检测具有互补作用,特别是对AFP阴性、低浓度的PHC更具有诊断价值.

  6. Herlyn-Werner-Wunderlich Syndrome: A Rare Cause of Pelvic Pain and High CA 19-9 Levels in an Adolescent Girl.

    Science.gov (United States)

    Unal, Emel; Tanyildiz, Hikmet Gulsah; Sonmezer, Murat; Erkol, Hatice Gul; Fitoz, Suat

    2016-01-01

    Herlyn-Werner-Wunderlich (HWW) syndrome is a rare developmental anomaly that includes uterus didelphys with obstructed hemivagina and ipsilateral renal agenesis. A 13-year-old girl presented with chronic abdominal pain. Magnetic resonance imaging revealed uterus didelphys, hematometrocolpos and renal agenesis on the right side with imperforate hymen. Subsequently the patient was found to have Mullerian duct anomalies. CA 19-9 level was high. At laparoscopy combined with vaginoscopy hematocolpos was drained following which she improved clinically and CA 19-9 level returned to normal. PMID:26816677

  7. Clinical significance of serum carbohydrate antigen 19-9 and its relationship with blood glucose in patients with type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    武秀玲

    2013-01-01

    Objective To study the relationship between serum carbohydrate antigen 19-9 (CA19-9) and blood glucose level in patients with type 2 diabetes (T2DM) .Methods Totally 784 T2DM patients and 197 healthy controls were enrolled in this study.Age,duration,body mass index (BMI) ,systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,levels of fasting blood glucose (FBG) ,postprandial blood glucose (PBG) ,hemoglobin A1c (HbA1c) ,total cholesterol (TC) ,triglyceride (TG) ,

  8. Herlyn-Werner-Wunderlich Syndrome: A Rare Cause of Pelvic Pain and High CA 19-9 Levels in an Adolescent Girl

    Directory of Open Access Journals (Sweden)

    Emel Unal

    2016-01-01

    Full Text Available Herlyn-Werner-Wunderlich (HWW syndrome is a rare developmental anomaly that includes uterus didelphys with obstructed hemivagina and ipsilateral renal agenesis. A 13-year-old girl presented with chronic abdominal pain. Magnetic resonance imaging revealed uterus didelphys, hematometrocolpos and renal agenesis on the right side with imperforate hymen. Subsequently the patient was found to have Müllerian duct anomalies. CA 19-9 level was high. At laparoscopy combined with vaginoscopy hematocolpos was drained following which she improved clinically and CA 19-9 level returned to normal.

  9. Measurement of a false electric dipole moment signal from $^{199}$Hg atoms exposed to an inhomogeneous magnetic field

    CERN Document Server

    Afach, S; Ban, G; Bison, G; Bodek, K; Chowdhuri, Z; Daum, M; Fertl, M; Franke, B; Geltenbort, P; Green, K; van der Grinten, M G D; Grujic, Z; Harris, P G; Heil, W; Hélaine, V; Henneck, R; Horras, M; Iaydjiev, P; Ivanov, S N; Kasprzak, M; Kermaïdic, Y; Kirch, K; Knowles, P; Koch, H -C; Komposch, S; Kozela, A; Krempel, J; Lauss, B; Lefort, T; Lemière, Y; Mtchedlishvili, A; Naviliat-Cuncic, O; Pendlebury, J M; Piegsa, F M; Pignol, G; Prashant, P N; Quéméner, G; Rebreyend, D; Ries, D; Roccia, S; Schmidt-Wellenburg, P; Severijns, N; Weis, A; Wursten, E; Wyszynski, G; Zejma, J; Zenner, J; Zsigmond, G

    2015-01-01

    We report on the measurement of a Larmor frequency shift proportional to the electric-field strength for $^{199}{\\rm Hg}$ atoms contained in a volume permeated with aligned magnetic and electric fields. This shift arises from the interplay between the inevitable magnetic field gradients and the motional magnetic field. The proportionality to electric-field strength makes it apparently similar to an electric dipole moment (EDM) signal, although unlike an EDM this effect is P- and T-conserving. We have used a neutron magnetic resonance EDM spectrometer, featuring a mercury co-magnetometer and an array of external cesium magnetometers, to measure the shift as a function of the applied magnetic field gradient. Our results are in good agreement with theoretical expectations.

  10. Double-neutron capture reaction and natural abundance of 183W, 195Pt, and 199Hg isotopes

    International Nuclear Information System (INIS)

    The database for neutron cross sections is well developed over the nuclide chart for stable isotopes and not as much for the radioactive species. Double-neutron capture experiments could be productive to supply more data. Time-integrated mean flux of slow neutrons reaches a value of 2.3·1012 cm-2·s-1 at the irradiation port near the active zone of the IBR-2 pulsed reactor of JINR. This is enough to detect the double-neutron capture products by the activation method. A high capture cross section is deduced in the present experiment for intermediate radioactive 182Ta and 194Ir target nuclides. Together with the known one for 198Au, these values may prove an essential role of double-neutron capture process for nucleosynthesis of 183W, 195Pt, and 199Hg isotopes at stellar conditions.

  11. Double-neutron capture reaction and natural abundance of 183W, 195Pt and 199Hg isotopes

    Science.gov (United States)

    Karamian, S. A.; Aksenov, N. V.; Bozhikov, G. A.

    2016-06-01

    There are much data on neutron cross sections over the chart of nuclides for stable isotopes and not as much for the radioactive ones. Double neutron capture experiments could be fruitful to provide more data. Time-integrated mean flux of slow neutrons reaches the value of 2.3-1012 n/cm2 s at the irradiation port near the active zone of the IBR-2 pulsed reactor of JINR. This is enough to detect the double neutron capture products by the activation method. A high capture cross section is obtained in the present experiment for intermediate radioactive 182Ta and 194Ir target nuclides. Together with the known data for 198Au, these values may prove an essential role of double neutron capture process for nucleosynthesis of 183W, 195Pt and 199Hg isotopes at stellar conditions.

  12. Teratoma during Pregnancy with Positive Estrogen and Progesterone Receptors and Elevated Ca19-9 Antigen Levels

    Directory of Open Access Journals (Sweden)

    Nilgun Kanlioglu Kuman

    2012-01-01

    Full Text Available We present a 27-year-old female patient admitted with an anterior mediastinal mass. She complained of chest discomfort and hemoptysis which began seven months prior. She had given birth five months prior. Thoracic X-ray showed an anterior mediastinal mass. Thorax computed tomography (CT confirmed a well-defined anterior mediastinal mass with  cm diameter, extending to the right hemithorax. It was composed of cystic spaces and discrete areas like soft tissue and fat. Serum Ca 19-9 level was elevated. CT features were consistent with a mature teratoma. During median sternotomy, the tumor revealed adhesions to the right lung and the right subclavian artery. Histologically, the tumor was diagnosed as a mature teratoma. Estrogen and progesterone receptors were detected to be positive in the resected tissue. We conclude that alterations in hormone levels during pregnancy might be the cause of rapid tumor growth which leads to hemoptysis.

  13. Multiconfiguration Dirac-Hartree-Fock calculations of atomic electric dipole moments of 225^Ra, 199^Hg, and 171^Yb

    CERN Document Server

    Radziute, Laima; Jonsson, Per; Biero, Jacek

    2013-01-01

    The multiconfiguration Dirac-Hartree-Fock (MCDHF) method has been employed to calculate atomic electric dipole moments (EDM) of 225^Ra, 199^Hg, and 171^Yb. For the calculations of the matrix elements we extended the relativistic atomic structure package GRASP2K. The extension includes programs to evaluate matrix elements of (P, T)-odd e-N tensor-pseudotensor and pseudoscalar-scalar interactions, the atomic electric dipole interaction, the nuclear Schiff moment, and the interaction of the electron electric dipole moment with nuclear magnetic moments. The interelectronic interactions were accounted for through valence and core-valence electron correlation effects. The electron shell relaxation was included with separately optimised wave functions of opposite parities.

  14. Measurement of a false electric dipole moment signal from 199Hg atoms exposed to an inhomogeneous magnetic field

    Science.gov (United States)

    Afach, S.; Baker, C. A.; Ban, G.; Bison, G.; Bodek, K.; Chowdhuri, Z.; Daum, M.; Fertl, M.; Franke, B.; Geltenbort, P.; Green, K.; van der Grinten, M. G. D.; Grujic, Z.; Harris, P. G.; Heil, W.; Hélaine, V.; Henneck, R.; Horras, M.; Iaydjiev, P.; Ivanov, S. N.; Kasprzak, M.; Kermaïdic, Y.; Kirch, K.; Knowles, P.; Koch, H.-C.; Komposch, S.; Kozela, A.; Krempel, J.; Lauss, B.; Lefort, T.; Lemière, Y.; Mtchedlishvili, A.; Naviliat-Cuncic, O.; Pendlebury, J. M.; Piegsa, F. M.; Pignol, G.; Prashant, P. N.; Quéméner, G.; Rebreyend, D.; Ries, D.; Roccia, S.; Schmidt-Wellenburg, P.; Severijns, N.; Weis, A.; Wursten, E.; Wyszynski, G.; Zejma, J.; Zenner, J.; Zsigmond, G.

    2015-10-01

    We report on the measurement of a Larmor frequency shift proportional to the electric-field strength for 199Hg atoms contained in a volume permeated with aligned magnetic and electric fields. This shift arises from the interplay between the inevitable magnetic field gradients and the motional magnetic field. The proportionality to electric-field strength makes it apparently similar to an electric dipole moment (EDM) signal, although unlike an EDM this effect is P- and T-conserving. We have used a neutron magnetic resonance EDM spectrometer, featuring a mercury co-magnetometer and an array of external cesium magnetometers, to measure the shift as a function of the applied magnetic field gradient. Our results are in good agreement with theoretical expectations.

  15. Primary epithelial splenic cyst with micro-rupture and raised carbohydrate antigen CA 19-9: a proposal for management

    Science.gov (United States)

    Papadopoulos, Iordanis N; Davatzikos, Anastasios; Kasabalis, Georgios; Manti, Christina; Konstantoudakis, Georgios

    2010-01-01

    Background Epithelial splenic cysts are rare entities which surgeons may not have previously encountered. Their management is based on historical studies. Case presentation A 21-year-old woman presented with a cyst of the spleen that produced a high serum concentration of carbohydrate antigen CA 19-9. A partial splenectomy with removal of the entire cyst and preservation of the splenic parenchyma by laparotomy was performed and the patient made an uneventful recovery. A microscopic rupture of the wall of the cyst and blood degradation products in the fluid of the cyst were confirmed. A functional splenic parenchyma was documented 18 months after surgery. There was no infection during follow-up. Conclusion Partial surgical splenectomy with resection of the entire cyst prevents recurrence and preserves splenic function. PMID:22791783

  16. Primary epithelial splenic cyst with micro-rupture and raised carbohydrate antigen CA 19-9: a paradigm of management

    Science.gov (United States)

    Papadopoulos, Iordanis N; Davatzikos, Anastasios; Kasabalis, Georgios; Manti, Christina; Konstantoudakis, Georgios

    2010-01-01

    Epithelial splenic cysts are rare entities that surgeons might not have previously encountered and their management is based on historical studies. A 21-year-old female presented with a cyst of the spleen that produced a high serum concentration of carbohydrate antigen CA 19-9. A partial splenectomy with the removal of the entire cyst and preservation of splenic parenchyma by laparotomy was performed and the patient made an uneventful recovery. A microscopic rupture of the wall of the cyst and degradation of blood in the fluid of the cyst were confirmed. Eighteen months following surgery a functional splenic parenchyma was documented. There was no episode of infection during this follow-up time. Partial surgical splenectomy with resection of the entire cyst prevents recurrence and preserves splenic function. PMID:22767471

  17. Environment Canada's new on-line reporting system for environmental emergency plans under CEPA, Sections 199 and 200

    International Nuclear Information System (INIS)

    One of the objectives of Canada's Environmental Emergencies program is to reduce the frequency, severity and consequences of spill events by promoting preventative measures and emergency preparedness. The program also offers advice on response methods and advancing science and technology. This paper from a poster session announces that the E2 program is expected to be administered online by late 2003. The compiled database is expected to serve as a useful statistical reference for the public, decision makers, and first responders. Users will be able to access information regarding controlled substances. In particular, sections 199 and 200 of the Environmental Emergency regulations in the Canadian Environmental Protection Act (CEPA) require that any person in Canada who manages a listed substance in large quantities provide Environment Canada with information on the quantity of the substance, the facility location, and prepare a contingency plan

  18. Biosynthesis of poly(3-hydroxybutyrate) and its copolymers by Yangia sp. ND199 from different carbon sources.

    Science.gov (United States)

    Huu Phong, Tran; Van Thuoc, Doan; Sudesh, Kumar

    2016-03-01

    A halophilic bacterium isolated from mangrove soil sample in Northern Vietnam, Yangia sp. ND199 was found capable of producing homopolymer poly(3-hydroxybutyrate) [P(3HB)], copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], and copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] from different carbon sources. The presence of 3HB, 3HV, and 4HB monomers were confirmed by gas chromatography and nuclear magnetic resonance analysis. Only P(3HB) was produced using carbon sources such as fructose or by a combination of fructose with 1,5-pentanediol, 1,6-hexanediol, sodium hexanoate, or sodium octanoate. The biosynthesis of P(3HB-co-3HV) was achieved by adding cosubstrates such as sodium valerate and sodium heptanoate. When 1,4-butanediol, γ-butyrolactone or sodium 4-hydroxybutyrate was added to the culture medium, P(3HB-co-4HB) containing 4.0-7.1mol% 4HB fraction was accumulated. The molecular weights and thermal properties of polyesters were determined by gel permeation chromatography and differential scanning calorimeter, respectively. The results showed that Yangia sp. ND199 is able to produce polyester with high weight average molecular weight ranging from 1.3×10(6) to 2.2×10(6) Dalton and number average molecular weight ranging from 4.2×10(5) to 6.9×10(5) Dalton. The molecular weights, glass transition temperature as well as melting temperature of homopolymer P(3HB) are higher than those of copolymer P(3HB-co-3HV) or P(3HB-co-4HB). PMID:26708435

  19. Utility of endoscopic ultrasound, cytology and fluid carcinoembryonic antigen and CA 19-9 levels in pancreatic cystic lesions

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To assess the diagnostic accuracy of endoscopic ultrasound (EUS), fluid tumor markers and cytology in distinguishing benign from (pre)malignant pancreatic cystic lesions.METHODS: 46 consecutive patients, referred to a gastroenterologist and surgeon for a symptomatic or incidental pancreatic cyst, were reviewed. EUS, cytology,and carcinoembryonic antigen (CEA) and carbohydrate antigen (CA 19-9) levels were compared with the final diagnosis, based on surgical pathology and/or imaging follow-up of at least 12 mo. Cysts were classified as benign (pseudocyst, serous cystadenoma) or malignant/pre-malignant (mucinous cystic neoplasm). Receiveroperator characteristics (ROC) curve analysis was performed.RESULTS: The mean age was 56 years; 29% were male and median cyst diameter was 3 cm. Final outcome was obtained in 41 (89%) patients. Twenty-three (56%) of these 41 had surgical pathology. Twenty-three (56%)had benign lesions and 18 (44%) had malignant/premalignant lesions. Sensitivity, specificity and positive and negative predictive value of EUS alone to distinguish benign from malignant/premalignant pancreatic cystic lesions were 50%, 56%, 36% and 54% and for cytology were 71%, 96%, 92% and 85%, respectively. The corresponding values for the ROC-derived ideal cutoffs were 75%, 90%, 75%, 90% for CA 19-9 (> 37 U/mL)and 70%, 85%, 79% and 78% for CEA (> 3.1 ng/mL).Subgroup analysis of those with surgical pathology yielded almost identical performance and cutoffs.CONCLUSION: Cytology and cyst fluid tumor marker analysis is a very useful tool in distinguishing benign from (pre)malignant pancreatic cystic lesions.

  20. Prognostic Significance of Carbohydrate Antigen 19-9 in Unresectable Locally Advanced Pancreatic Cancer Treated With Dose-Escalated Intensity Modulated Radiation Therapy and Concurrent Full-Dose Gemcitabine: Analysis of a Prospective Phase 1/2 Dose Escalation Study

    International Nuclear Information System (INIS)

    Purpose: Although established in the postresection setting, the prognostic value of carbohydrate antigen 19-9 (CA19-9) in unresectable locally advanced pancreatic cancer (LAPC) is less clear. We examined the prognostic utility of CA19-9 in patients with unresectable LAPC treated on a prospective trial of intensity modulated radiation therapy (IMRT) dose escalation with concurrent gemcitabine. Methods and Materials: Forty-six patients with unresectable LAPC were treated at the University of Michigan on a phase 1/2 trial of IMRT dose escalation with concurrent gemcitabine. CA19-9 was obtained at baseline and during routine follow-up. Cox models were used to assess the effect of baseline factors on freedom from local progression (FFLP), distant progression (FFDP), progression-free survival (PFS), and overall survival (OS). Stepwise forward regression was used to build multivariate predictive models for each endpoint. Results: Thirty-eight patients were eligible for the present analysis. On univariate analysis, baseline CA19-9 and age predicted OS, CA19-9 at baseline and 3 months predicted PFS, gross tumor volume (GTV) and black race predicted FFLP, and CA19-9 at 3 months predicted FFDP. On stepwise multivariate regression modeling, baseline CA19-9, age, and female sex predicted OS; baseline CA19-9 and female sex predicted both PFS and FFDP; and GTV predicted FFLP. Patients with baseline CA19-9 ≤90 U/mL had improved OS (median 23.0 vs 11.1 months, HR 2.88, P<.01) and PFS (14.4 vs 7.0 months, HR 3.61, P=.001). CA19-9 progression over 90 U/mL was prognostic for both OS (HR 3.65, P=.001) and PFS (HR 3.04, P=.001), and it was a stronger predictor of death than either local progression (HR 1.46, P=.42) or distant progression (HR 3.31, P=.004). Conclusions: In patients with unresectable LAPC undergoing definitive chemoradiation therapy, baseline CA19-9 was independently prognostic even after established prognostic factors were controlled for, whereas CA19-9 progression

  1. An Ultrasensitive Electrochemiluminescence Immunoassay for Carbohydrate Antigen 19-9 in Serum Based on Antibody Labeled Fe3O4 Nanoparticles as Capture Probes and Graphene/CdTe Quantum Dot Bionanoconjugates as Signal Amplifiers

    Directory of Open Access Journals (Sweden)

    Ning Gan

    2013-05-01

    Full Text Available The CdTe quantum dots (QDs, graphene nanocomposite (CdTe-G and dextran–Fe3O4 magnetic nanoparticles have been synthesized for developing an ultrasensitive electrochemiluminescence (ECL immunoassay for Carcinoembryonic antigen 19-9 (CA 19-9 in serums. Firstly, the capture probes (CA 19-9 Ab1/Fe3O4 for enriching CA 19-9 were synthesized by immobilizing the CA 19-9’s first antibody (CA 19-9 Ab1 on magnetic nanoparticles (dextran-Fe3O4. Secondly, the signal probes (CA 19-9 Ab2/CdTe-G, which can emit an ECL signal, were formed by attaching the secondary CA 19-9 antibody (CA 19-9 Ab2 to the surface of the CdTe-G. Thirdly, the above two probes were used for conjugating with a serial of CA 19-9 concentrations. Graphene can immobilize dozens of CdTe QDs on their surface, which can emit stronger ECL intensity than CdTe QDs. Based on the amplified signal, ultrasensitive antigen detection can be realized. Under the optimal conditions, the ECL signal depended linearly on the logarithm of CA 19-9 concentration from 0.005 to 100 pg/mL, and the detection limit was 0.002 pg/mL. Finally, five samples of human serum were tested, and the results were compared with a time-resolved fluorescence assay (TRFA. The novel immunoassay provides a stable, specific and highly sensitive immunoassay protocol for tumor marker detection at very low levels, which can be applied in early diagnosis of tumor.

  2. 41 CFR 101-31.200 - Cross-reference to the Federal Acquisition Regulation (FAR) (48 CFR chapter 1, parts 1-99).

    Science.gov (United States)

    2010-07-01

    ... Federal Acquisition Regulation (FAR) (48 CFR chapter 1, parts 1-99). 101-31.200 Section 101-31.200 Public... Grading Services § 101-31.200 Cross-reference to the Federal Acquisition Regulation (FAR) (48 CFR chapter... 46) (48 CFR Subpart 7.5, and parts 37 and 46)....

  3. miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes

    Directory of Open Access Journals (Sweden)

    Keita Tsujimura

    2015-09-01

    Full Text Available Rett syndrome (RTT is a neurodevelopmental disorder caused by MECP2 mutations. Although emerging evidence suggests that MeCP2 deficiency is associated with dysregulation of mechanistic target of rapamycin (mTOR, which functions as a hub for various signaling pathways, the mechanism underlying this association and the molecular pathophysiology of RTT remain elusive. We show here that MeCP2 promotes the posttranscriptional processing of particular microRNAs (miRNAs as a component of the microprocessor Drosha complex. Among the MeCP2-regulated miRNAs, we found that miR-199a positively controls mTOR signaling by targeting inhibitors for mTOR signaling. miR-199a and its targets have opposite effects on mTOR activity, ameliorating and inducing RTT neuronal phenotypes, respectively. Furthermore, genetic deletion of miR-199a-2 led to a reduction of mTOR activity in the brain and recapitulated numerous RTT phenotypes in mice. Together, these findings establish miR-199a as a critical downstream target of MeCP2 in RTT pathogenesis by linking MeCP2 with mTOR signaling.

  4. MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199.

    Science.gov (United States)

    Benito, Juliana M; Godfrey, Laura; Kojima, Kensuke; Hogdal, Leah; Wunderlich, Mark; Geng, Huimin; Marzo, Isabel; Harutyunyan, Karine G; Golfman, Leonard; North, Phillip; Kerry, Jon; Ballabio, Erica; Chonghaile, Triona Ní; Gonzalo, Oscar; Qiu, Yihua; Jeremias, Irmela; Debose, LaKiesha; O'Brien, Eric; Ma, Helen; Zhou, Ping; Jacamo, Rodrigo; Park, Eugene; Coombes, Kevin R; Zhang, Nianxiang; Thomas, Deborah A; O'Brien, Susan; Kantarjian, Hagop M; Leverson, Joel D; Kornblau, Steven M; Andreeff, Michael; Müschen, Markus; Zweidler-McKay, Patrick A; Mulloy, James C; Letai, Anthony; Milne, Thomas A; Konopleva, Marina

    2015-12-29

    Targeted therapies designed to exploit specific molecular pathways in aggressive cancers are an exciting area of current research. Mixed Lineage Leukemia (MLL) mutations such as the t(4;11) translocation cause aggressive leukemias that are refractory to conventional treatment. The t(4;11) translocation produces an MLL/AF4 fusion protein that activates key target genes through both epigenetic and transcriptional elongation mechanisms. In this study, we show that t(4;11) patient cells express high levels of BCL-2 and are highly sensitive to treatment with the BCL-2-specific BH3 mimetic ABT-199. We demonstrate that MLL/AF4 specifically upregulates the BCL-2 gene but not other BCL-2 family members via DOT1L-mediated H3K79me2/3. We use this information to show that a t(4;11) cell line is sensitive to a combination of ABT-199 and DOT1L inhibitors. In addition, ABT-199 synergizes with standard induction-type therapy in a xenotransplant model, advocating for the introduction of ABT-199 into therapeutic regimens for MLL-rearranged leukemias. PMID:26711339

  5. MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199

    Directory of Open Access Journals (Sweden)

    Juliana M. Benito

    2015-12-01

    Full Text Available Targeted therapies designed to exploit specific molecular pathways in aggressive cancers are an exciting area of current research. Mixed Lineage Leukemia (MLL mutations such as the t(4;11 translocation cause aggressive leukemias that are refractory to conventional treatment. The t(4;11 translocation produces an MLL/AF4 fusion protein that activates key target genes through both epigenetic and transcriptional elongation mechanisms. In this study, we show that t(4;11 patient cells express high levels of BCL-2 and are highly sensitive to treatment with the BCL-2-specific BH3 mimetic ABT-199. We demonstrate that MLL/AF4 specifically upregulates the BCL-2 gene but not other BCL-2 family members via DOT1L-mediated H3K79me2/3. We use this information to show that a t(4;11 cell line is sensitive to a combination of ABT-199 and DOT1L inhibitors. In addition, ABT-199 synergizes with standard induction-type therapy in a xenotransplant model, advocating for the introduction of ABT-199 into therapeutic regimens for MLL-rearranged leukemias.

  6. Distinct roles of enhancer nuclear factor 1 (NF1) sites in plasmacytoma and osteopetrosis induction by Akv1-99 murine leukemia virus

    International Nuclear Information System (INIS)

    Murine leukemia viruses (MLVs) can be lymphomagenic and bone pathogenic. In this work, the possible roles of two distinct proviral enhancer nuclear factor 1 (NF1) binding sites in osteopetrosis and tumor induction by B-lymphomagenic Akv1-99 MLV were investigated. Akv1-99 and mutants either with NF1 site 1, NF1 site 2 or both sites disrupted induced tumors (plasma cell proliferations by histopathology) with remarkably similar incidence and mean latency in inbred NMRI mice. Clonal immunoglobulin gene rearrangement detection, by Southern analysis, confirmed approximately half of the tumors induced by each virus to be plasmacytomas while the remaining lacked detectable clonally rearranged Ig genes and were considered polyclonal; a demonstration that enhancer NF1 sites are dispensable for plasmacytoma induction by Akv1-99. In contrast, X-ray analysis revealed significant differences in osteopetrosis induction by the four viruses strongly indicating that NF1 site 2 is critical for viral bone pathogenicity, whereas NF1 site 1 is neutral or moderately inhibitory. In conclusion, enhancer NF1 sites are major determinants of osteopetrosis induction by Akv1-99 without significant influence on viral oncogenicity

  7. 血清CEA、CA19-9及铁蛋白联合检测在胰腺癌诊断中的价值%The Diagnostic Value of Serum CEA and CA19-9 and Ferritin in the Diagnosis of Pancreatic Cancer

    Institute of Scientific and Technical Information of China (English)

    高迎

    2015-01-01

    Objective To explore the value of the combination detection of serum CEA, CA19-9 and ferritin in the diagnosis of pancreatic cancer. Methods 58 patients conducted pancreatic cancer (pancreatic caner group), other 58 patients with chronic pancreatitis (chronic pancreatitis group) and 58 healthy persons conducted physical examination (control group) in our hospital were recruited for this study, serum CEA, CA19-9 and ferritin expression levels in each group and detection sensitivity and specificity were compared.Results The serum CEA, CA19-9 and ferritin expression levels in the pancreatic cancer group were signiifcantly higher than those in the chronic pancreatitis group and the control group, the differences had statistical signiifcance (P<0.05), the serum CEA, CA19-9 and ferritin expression levels in the patients with the survival period not less than 6months were lower than those in the patients with the survival period less than 6 months, the differences had statistical significance (P<0.05), the sensitivity and the specificity of the combination detection of CEA, CA19-9 and ferritin were 87.2% and 96.1% respectively.Conclusion The combination detection of serum CEA, CA19-9 and ferritin could have important reference value for the diagnosis of pancreatic cancer.%目的:探讨血清癌胚抗原(CEA)、糖类抗原19-9(CA19-9)及铁蛋白联合检测在胰腺癌诊断中的价值。方法选取南阳市中心医院住院治疗且确诊的胰腺癌患者58例(胰腺癌组),另选取慢性胰腺炎患者58例(慢性胰腺炎组)及体检健康者58例(对照组),比较各组血清CEA、CA19-9及铁蛋白水平及其检测敏感性和特异性。结果胰腺癌患者血清CEA、CA19-9及铁蛋白的表达水平均高于慢性胰腺炎组和对照组,差异均有统计学意义(P<0.05),生存期不低于6个月的患者血清CEA、CA19-9及铁蛋白表达水平均低于生存期少于6个月的患者,比较差

  8. Metabolic tumour burden assessed by {sup 18}F-FDG PET/CT associated with serum CA19-9 predicts pancreatic cancer outcome after resection

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Hua-Xiang; Chen, Tao; Wang, Wen-Quan; Wu, Chun-Tao; Liu, Chen; Long, Jiang; Xu, Jin; Liu, Liang; Yu, Xian-Jun [Fudan University, Shanghai Cancer Center, Pancreatic Cancer Institute and Department of Pancreatic and Hepatobiliary Surgery, Shanghai (China); Fudan University, Department of Oncology, Shanghai Medical College, Shanghai (China); Zhang, Ying-Jian [Fudan University, Shanghai Cancer Center, Department of Nuclear Medicine, Shanghai (China); Fudan University, Department of Oncology, Shanghai Medical College, Shanghai (China); Chen, Run-Hao [Fudan University, Department of General Surgery, Jinshan Hospital, Shanghai (China)

    2014-06-15

    Tumour burden is one of the most important prognosticators for pancreatic ductal adenocarcinoma (PDAC). The aim of this study was to investigate the predictive significance of metabolic tumour burden measured by {sup 18}F-FDG PET/CT in patients with resectable PDAC. Included in the study were 122 PDAC patients who received preoperative {sup 18}F-FDG PET/CT examination and radical pancreatectomy. Metabolic tumour burden in terms of metabolic tumour volume (MTV) and total lesion glycolysis (TLG), pathological tumour burden (tumour size), serum tumour burden (baseline serum CA19-9 level), and metabolic activity (maximum standard uptake value, SUVmax) were determined, and compared for their performance in predicting overall survival (OS) and recurrence-free survival (RFS). MTV and TLG were significantly associated with baseline serum CA19-9 level (P = 0.001 for MTV, P < 0.001 for TLG) and tumour size (P < 0.001 for MTV, P = 0.001 for TLG). Multivariate analysis showed that MTV, TLG and baseline serum CA19-9 level as either categorical or continuous variables, but not tumour size or SUVmax, were independent risk predictors for both OS and RFS. Time-dependent receiving operating characteristics analysis further indicated that better predictive performances for OS and RFS were achieved by MTV and TLG compared to baseline serum CA19-9 level, SUVmax and tumour size (P < 0.001 for all). MTV and TLG showed strong consistency with baseline serum CA19-9 level in better predicting OS and RFS, and might serve as surrogate markers for prediction of outcome in patients with resectable PDAC. (orig.)

  9. Clinical value of combining tumor maker alphafetoprotein(AFP),CA125 and CA19-9 in diagnose of liver cirrhosis and PHC%血清AFP、CA125、CA19-9联检在肝硬化和肝癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    胡兰英; 徐笛; 毕波

    2008-01-01

    目的:探讨肿瘤标志物甲胎蛋白(AFP)、CA125、CA19-9在肝硬化和原发性肝癌(PHC)中的诊断价值.方法:对PHC组50例、肝硬化组40例、肝炎组45例和正常对照组30例,检测血清AFP、CA125和CA19-9水平(前三组患者在接受治疗前检测).结果:PHC组血清AFP水平均明显高于肝炎组及正常对照组,差异有非常显著性(P<0.05).PHC组血清AFP、CA125和CA19-9阳性率分别为70.0%、82.O%、90.0%,CA125、AFP联检阳性率为91.2%, AFP、CA125与CA19-9联检阳性率达97.3%.结论:血清AFP、CA125和CA19-9联合检测具有互补作用,可提高PHC的诊断率,特别是对AFP阴性、低浓度的PHC更具有诊断价值.

  10. The value of serum CA19-9 levels before and after relief of jaundice in differentiating benign from malignant etiologies of obstructive jaundice%减黄前后血清CA19-9测定对梗阻性黄疸良恶性病因的鉴别诊断价值

    Institute of Scientific and Technical Information of China (English)

    黄家淼; 杨雅; 杨剑; 王琴

    2012-01-01

    目的 探讨良、恶性梗阻性黄疸患者减黄治疗前后血清CA19-9的变化规律及其对良恶性病因的鉴别诊断价值.方法 回顾性分析2006年10月至2011年10月我院收治的97例良性和93例恶性梗阻性黄疸患者的临床资料和血清CA19-9测定结果.结果 97例良性和93例恶性梗阻性黄疸患者减黄治疗前的血清CA19-9均值、阳性率分别为(70.7±58.2) U/ml和(411.0±257.5) U/ml、64.9%和81.7%;减黄治疗前的血清CA19-9诊断恶性梗阻性黄疸的灵敏度为81.7%,特异度为35.1%,准确性为57.9%.血清CA19-9升高的29例良性和34例恶性梗阻性黄疸患者经减黄治疗后2周,良性组血清CA19-9由(271.5±93.7)U/ml降至(30.5±21.6) U/ml(P =0.000),其中降至正常者20例,仍高于正常值但下降幅度>50%者9例;而恶性组血清CA19-9减黄前后无明显变化.良性组减黄前、后血清CA19-9水平均与血清总胆红素水平呈正相关(r =0.572,P=0.001;r=0.350,P=0.043);而恶性组减黄前、后血清CA19-9水平均与血清总胆红素水平无关(r=0.125,P=0.517;r=0.05,P =0.817).结论 减黄治疗前血清CA19-9测定对梗阻性黄疸良、恶性病因的鉴别诊断意义有限,然而有效减黄后血清CA19-9的变化能够较好地鉴别梗阻性黄疸良、恶性病因.%Objective To observe the changes of serum CA19-9 level before and alter relief of jaundice and explore the value in differentiating benign from malignant etiologies of obstructive jaundice. Methods The CA19-9 serum levels of ninety-seven patients with benign and ninety-three patients with malignant obstructive jaundice admitted to our hospital during October 2006 to October 2011 were analyzed retrospectively. Results The mean and positive rates of the serum CA19-9 levels were (70. 7 ± 58. 2) U/ml and (411.0 ± 257. 5) U/ml, 64. 9% and 81.1% in 97 cases benign and 93 cases malignant obstructive jaundice. The overlap was substantial with sensitivity of 81. 7% , specificity

  11. 一种新型免疫传感器阵列检测癌胚抗原和糖类抗原199技术%A New Type of Sensor Array Detection of Carcinoembryonic Antigen and Carbohydrate Antigen 199 Technology

    Institute of Scientific and Technical Information of China (English)

    周靖; 胡富陶; 余红卫

    2015-01-01

    制备了一种新型的含2个包覆分子印迹聚合物的丝网印刷碳工作电极的电化学发光夹心免疫传感器阵列,通过该阵列实现了肿瘤标志物癌胚抗原(CEA)和糖类抗原199(CA199)的近同时免疫分析。该新型的电化学发光免疫方法将空间分辨技术与分子印迹技术相结合,为 CEA 和CA199的复合免疫分析提供了一条简单、低成本、快速和灵敏的途径。该方法对临床实验中多种蛋白质的近同时测定也显示了较大的潜力。%In this study, a novel electrochemiluminescence (ECL) immunosensor array is engineered consisting of two screen-printed carbon working electrodes coated with molecular imprinting polymer (MIPs). The array is formed to perform multiplexed immunoassay of tumor markers (carcinoembryonic antigen (CEA) and carbohydrate antigen-199 (CA199)). Based on spatial-resolved technique, and coupled with molecularly imprinted technique, this novel ECL strategy provides a simple, low-cost, fast and sensitive approach for multiplexed immunoassay of CEA and CA199. This approach may render promising potential for protein detection in the circumstances of clinical laboratory setting.

  12. TUMOR MARKER CA125、 CA153、 CA199 AND CEA IN LUNG CANCER%肿瘤标记物CA125、CA153、CA199与CEA 在肺癌诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    谢汉华

    2001-01-01

    目的:研究四种肿瘤标记物CA125、CA153、CA199与CEA在肺癌诊断中的意义,并以常见的几种呼吸道炎症性疾病(RIDs)作为对照。结果:肺癌患者CA125、CA153、CA199及CEA水平均显著高于RIDs患者(P<0.001),而CA125、CA153、CA199及CEA水平在肺癌的三种组织学类型和各期肺癌中并显著性差异(P>0.05)。采有四项指标的平均值作为标准,虽然并不能显著提高诊断的灵敏度(P>0.05),但可显著提高诊断的特异性(P<0.05或0.01)。结论:CA125、CA153、CA199及CEA可作为肺癌的标记物,检测这四项标记物在临床上具有一定的辅助意义。%Four tumor markers,CA125,CA153,CA199 and CEA are studied for their relations with lung cancer,using Respiratory Inflammatory Diseases,RIDs (bronchitis,bronchiectasis and pneumonia)as control.The result shows that the serum levels of CA125,CA153,CA199 and CEA are significantly higher in the patients with lung cancer than in those with RIDs(P<0.001).There are not significant differences of the serum level of CA125,CA153,CA199 and in different tissular types of lung cancer and at different phases of lung cancer (P>0.05).The mean value of the four markers is used for a new standard for judging lung cancer.The sensitivity is not significantly higher,but the specificity is significantly lifted(P<0.05 or 0.01).It seems that CA125,CA153,CA199 and CEA can be useful markers for lung cancer,and that the detection of the four markers can play assistant role in the judgement of lung cancer.

  13. 血清CEA、CA125、CA199、CA153联检在肺癌中的诊断价值%The value of joint detection of serum CEA, CA125, CA199 and CA153 in diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    王露; 徐红珍

    2009-01-01

    目的 评价血清CEA、CA125、CA199、CA153联合检测对肺癌的诊断价值.方法 用化学发光法测定45例肺癌患者,50例肺良性疾病患者及30例健康体检者血清CEA、CA125、CA199、CA153水平的变化,评价4项指标联合应用的诊断价值.结果 肺癌组血清CEA、CA125、CA199、CA153含量明显高于对照组(P<0.01),单项肿瘤指标CEA、CA125、CA199、CA153的检测敏感性分别为53.3%、48.9%、35.6%、37.8%.联合检测可提高检测的敏感性达87.6%.结论 肿瘤标志物CEA、CA125、CA199、CA153的联合检测可提高肺癌的诊断率,可为肺癌的早期诊断提供有价值的资料.%Objective To evaluate the clinical value of measuring the four tumor makers(CEA,CA125,CA199,CA153)for diagnosis of lung cancer. Methods These tumor markers in serum were measured with chemiluminescence immunoassay in 45 patients with lung cancer, 50 benign lung diseases and 30 healthy subjects. Results The level of the markers in lung cancer group were all higher than those of benign lung diseases and healthy subjects. The sensitivity of single tumor marker (CEA,CA125,CA199,CA153)was 53.3%,48.9%,35.6%,37.8% respectively. The sensitivity of combined measurement was 87.6%. Conclusion The combined measurement of serum CEA,CA125,CA199,CA153 significantly increase the sensitivity for lung cancer, and provide useful information for early diagnosis of disease in patients with lung cancer.

  14. Combined detection of serum CEA, CA199, CA724 application value in patients with colorectal cancer TNM staging%血清CA199、CA724、CEA联合检测在结直肠癌患者TNM分期中的应用

    Institute of Scientific and Technical Information of China (English)

    林评跃; 李夏鲁

    2015-01-01

    目的:探讨血清糖类抗原CA199糖类抗原CA724癌胚抗原CEA在结直肠癌的TNM分期中的临床应用价值。方法:观察组结直肠癌患者100例与对照组同期非消化道肿瘤的住院病人100例,空腹抽取静脉血,采用罗氏公司E601电化学发光全自动免疫分析仪和罗氏原装试剂盒检测血清中CEA、CA199、CA724值。应用SPSS 17.0软件进行统计学处理,计量资料以均数±标准差f ±s)表示,采用单因素方差分析进行统计,计数资料采用X-2检验,P<0.05为差异有统计学意义。结果:在单项检测中,CEA的敏感率最高,达37%,且CEA>CA199>CA724.在两项联合检测中CA199+CEA的敏感度最高达53%,三项联合检测敏感度达64%,在各种联合检测中最高。三项肿瘤标志物的阳性率和升高水平随肿瘤的TNM分期的增加而递增。结论:三项肿瘤标志物的单一检测敏感度较低,肿瘤标志的联合检测结直肠癌可以提高敏感性,以CEA+CA199+CA724的效果最好,CA199+CEA次之。CA199、CA724、CEA三项肿瘤标志物的阳性率及升高水平依肿瘤的TNM分期的增加呈递增趋势。%Objective: To investigate the combined detection of serum CEA, CA199, CA724 application value in patients with colorectal cancer TNM staging.Methods:Venous bloods were collected from 100 patients with colorectal cancer and 100 healthy control cases of hospitalized patients during the same period of the digestive tract tumor. The Serum levels of CEA, CA199 and CA724 lwere determined by chemiluminescence immunoassay. All data are shown as mean X ±SD for the indicated number of independently performed experiments. All data were analyzed using the SPSS package for Windows(Version 17.0). Differences with P<0.05 were considered statistically significant.Results:The sensitivity of single tumor maker in the diagnosis of CEA was 37%, higher than CA199 and CA724 was less than CA199. The sensitivity of combined

  15. Astatination of closo-dodecaborate(2-) anion

    Czech Academy of Sciences Publication Activity Database

    Orlova, A.; Lebeda, Ondřej; Tolmachev, V.; Lundquist, H.; Carlsson, J.; Sjöberg, S.

    Cambridge : The Royal Society of Chemistry , 2000 - (Davidson, M.; Hughes, A.; Marder, T.; Wade , K.), s. 144-147 [Conference "Contemporary Boron Chemistry ". Durham (GB), 11.07.1999-15.07.1999] Institutional research plan: CEZ:AV0Z1048901 Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders

  16. Herlyn-Werner-Wunderlich Syndrome: A Rare Cause of Pelvic Pain and High CA 19-9 Levels in an Adolescent Girl

    OpenAIRE

    Emel Unal; Hikmet Gulsah Tanyildiz; Murat Sonmezer; Hatice Gul Erkol; Suat Fitoz

    2016-01-01

    Herlyn-Werner-Wunderlich (HWW) syndrome is a rare developmental anomaly that includes uterus didelphys with obstructed hemivagina and ipsilateral renal agenesis. A 13-year-old girl presented with chronic abdominal pain. Magnetic resonance imaging revealed uterus didelphys, hematometrocolpos and renal agenesis on the right side with imperforate hymen. Subsequently the patient was found to have Müllerian duct anomalies. CA 19-9 level was high. At laparoscopy combined with vaginoscopy hematocolp...

  17. The Good Business of Language Learning: LWB 199, a Gateway Course to the Language and Business Major at the University of Tennessee

    OpenAIRE

    Kaplan, Gregory B.

    2010-01-01

    Language and World Business is a major at the University of Tennessee that combines one of eight language tracks with a specialization in one of three professional emphasis concentrations. During the course of completing this major, students also study abroad or perform an internship. The present study explores the format and content of Language and World Business 199, a required course that involves students in activities that introduce them to the curriculum and a variety of study abroad an...

  18. Tissue expression of the tumour associated antigen CA242 in benign and malignant pancreatic lesions. A comparison with CA 50 and CA 19-9.

    OpenAIRE

    Haglund, C.; Lindgren, J.; Roberts, P. J.; Kuusela, P.; Nordling, S.

    1989-01-01

    The expression of a novel tumour associated antigen CA 242, defined by the monoclonal antibody C 242, was studied by immunoperoxidase staining in formalin-fixed, paraffin-embedded tissue sections from normal pancreata, pancreata with pancreatitis and benign and malignant pancreatic neoplasms. The antigenic determinant of the C 242 antibody is a sialylated carbohydrate structure, related but chemically different from tumour marker antigens CA 19-9 and CA 50. Thirty-eight of 41 (93%) well to mo...

  19. The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran

    International Nuclear Information System (INIS)

    Since several high level natural radiation areas (HLNRAs) exist on our planet, considerable attention has been drawn to health issues that may develop as the result of visiting or living in such places. City of Ramsar in Iran is an HNLRA, and is a tourist attraction mainly due to its hot spas. However, the growing awareness over its natural radiation sources has prompted widespread scientific investigation at national level. In this study, using an ELISA method, the level of expression of three tumor markers known as carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and carcino antigen 19-9 (CA19-9) in blood serum of 40 local men of Ramsar (subject group) was investigated and compared to 40 men from the city of Noshahr (control group). Noshahr was previously identified as a normal level natural radiation area (NLNRA) that is some 85 km far from Ramsar. According to statistical analysis, there was a significant difference in the levels of PSA and CA19-9 markers between the two groups (p < 0.001) with those of Ramsar being considerably higher. CEA level did not show any difference. Although some of the volunteers tested positive to the markers, they were in good health as confirmed by the physician. Moreover, the high number of positive markers in Noshahr was considerable. Therefore, future study is needed to further validate this result and to determine the level of positivity to tumor markers in both cities. -- Highlights: • Expression of three tumor markers was examined in 80 volunteers from Ramsar. • There was a significant difference in the levels of PSA and CA19-9 markers. • Some of the volunteers from the control city of Noshahr also tested positive. • Radiation might have caused an adaptive response in people of Ramsar. • Further study is necessary to re-confirm the positivity to tumor marker

  20. Silver-functionalized g-C3N4 nanohybrids as signal-transduction tags for electrochemical immunoassay of human carbohydrate antigen 19-9.

    Science.gov (United States)

    Sun, Ai-Li; Qi, Qing-An

    2016-07-21

    A simple and feasible electrochemical immunosensing platform was developed for highly efficient screening of a disease-related protein (human carbohydrate antigen 19-9, CA 19-9 used in this case) using silver-functionalized g-C3N4 nanosheets (Ag/g-C3N4) as signal-transduction tags. Initially, Ag/g-C3N4 nanohybrids were synthesized by combining thermal polymerization of the melamine precursor with the photo-assisted reduction method. Thereafter, the as-synthesized Ag/g-C3N4 nanohybrids were utilized for the labeling of the anti-CA 19-9 detection antibody by using a typical carbodiimide coupling method. The assay was carried out on a capture antibody-modified glassy carbon electrode in a sandwich-type detection mode. The detectable signal mainly derived from the voltammetric characteristics of the immobilized nanosilver particles on the g-C3N4 nanosheets within the applied potentials. Under the optimal conditions, the voltammetric peak currents increased with the increasing amount of target CA 19-9, and exhibited a wide linear range from 5.0 mU mL(-1) to 50 U mL(-1) with a detection limit of 1.2 mU mL(-1). Our strategy also displayed good reproducibility, precision and specificity. The results of the analysis of clinical serum specimens were in good accordance with the results obtained by an enzyme-linked immunosorbent assay (ELISA) method. The newly developed immunosensing system is promising for enzyme-free and cost-effective analysis of low-abundance proteins. PMID:27183220

  1. Pretreatment Carbohydrate Antigen 19-9 Level Indicates Tumor Response, Early Distant Metastasis, Overall Survival, and Therapeutic Selection in Localized and Unresectable Pancreatic Cancer

    International Nuclear Information System (INIS)

    Purpose: The use of chemoradiotherapy (CRT) for localized and unresectable pancreatic cancer has been disputed because of high probability of distant metastasis. Thus, we analyzed the effect of clinical parameters on tumor response, early distant metastasis within 3 months (DM3m), and overall survival to identify an indicator for selecting patients who would benefit from CRT. Methods and Materials: This study retrospectively analyzed the data from 84 patients with localized and unresectable pancreatic cancer who underwent CRT between August 2002 and October 2009. Sex, age, tumor size, histological differentiation, N classification, pre- and post-treatment carbohydrate antigen (CA) 19-9 level, and CA 19-9 percent decrease were analyzed to identify risk factors associated with tumor response, DM3m, and overall survival. Results: For all 84 patients, the median survival time was 12.5 months (range, 2–31.9 months), objective response (complete response or partial response) to CRT was observed in 28 patients (33.3%), and DM3m occurred in 24 patients (28.6%). Multivariate analysis showed that pretreatment CA 19-9 level (≤400 vs. >400 U/ml) was significantly associated with tumor response (45.1% vs. 15.2%), DM3m (19.6% vs. 42.4%), and median overall survival time (15.1 vs. 9.7 months) (p 3m, and overall survival and identifying patients who will benefit from CRT.

  2. Tissue CA-19.9 content in colorectal adenomas and its value in the assesment of dysplasia Detección de CA-19.9 citosólico en adenomas colorrectales: Utilidad en el diagnóstico de la displasia

    Directory of Open Access Journals (Sweden)

    I. Salces

    2004-04-01

    Full Text Available Background: occasionally, the risk of malignant transformation may be difficult to establish in adenomatous polyps due to the fact that they contain areas with variable grades of dysplasia. A measurement of tissue tumor markers may be useful to recognize these adenomas. Objectives: the aims of this study were: to establish firstly the relationship between carbohydrate antigen 19.9 (CA-19.9 content in the colorectal mucosa and the characteristics of polyps, and secondly, the diagnostic value of the former's measurement. Patients and methods: tissue CA-19.9 concentration was measured in 155 colorectal samples obtained from 145 patients (21 normal mucosa; 113 adenomatous polyps; 21 adenocarcinoma. Cytosol CA-19.9 content was determined by enzyme-linked immunoadsorbant assay, and the measurement of this protein was achieved by quantitative assay. Tissue samples were also processed for histological examination. Results: we demonstrated that CA-19.9 levels in adenomatous polyps and adenocarcinomas were significantly higher than in the normal mucosa. These levels varied significantly according to polyp size, histological type, and grade of dysplasia. CA-19.9 contents were higher in polyps with a high risk of malignant transformation than in those with a low risk of severe dysplasia. The cut-off value 214 U/mg of protein properly differentiated both types of risk. The area under the receiver operating characteristic (ROC curves showed that cytosol CA-19.9 levels allow classifying polyps according to their histological features. Conclusions: we concluded that the measurement of CA-19.9 content in adenomatous polyps may be useful to classify these tumors and confirm the feasibility to separate adenomas into two groups: low and high risk of malignant change.Introducción: en ocasiones, el riesgo de transformación maligna de los pólipos adenomatosos es difícil de identificar por existir zonas con distintos grados de displasia simultáneamente. La

  3. 瘢痕疙瘩microRNA表达谱的筛选及miR-199a-5p生物功能的初步研究%Identification of differently expressed microRNAs in keloid and pilot study on biological function of miR-199a-5p

    Institute of Scientific and Technical Information of China (English)

    吴志远; 卢玲; 郭晓瑞; 张培华

    2013-01-01

    Objective To screen out related microRNAs in keloid tissue,and identify their effect on the proliferation of keloid fibroblasts.Methods 8 cases of keloid tissue and 8 cases of normal skin tissue were collected as specimens.The differently expressed miRNA in keloid tissue from normal skin tissue were screened out with gene chip(Exiqon company),which was validated with quantitative real-time PCR.Then miRNA mimics was transfected into keloid fibroblasts line to stimulate high expression of mature miRNA in cells.The effect on the proliferation of fibroblasts in keloid was tested by Edu.Results ①A total of 17 differently expressed microRNAs were found,including miR-199a-5p.②The expression of miR-199a-5p had been verified by qRT-PCR to be down-regulated in keloid,which was consistent with the result of array.③The positive rate of EdU in miR-199a-5p mimics transfected group and negative control group was (20.72 ± 2.50)% and (27.68 ± 4.92)%,respectively.The proliferative rate of keloid fibroblasts turned down in miR-199a-5p-transfected group (t =2.183,P =0.047).Besides that,the cell cycle changed after transfection.The percentage of S and G2/M phase in miR-199a-5p mimics transfected group was 33.93 ± 1.30 and 10.87 ± 0.80,respectively,while it was 31.39 ± 0.79 and 9.27 ± 0.46 in negative control group,and the difference was statistically significant.Conclusions ① The miRNA expression profile is different between keloid and normal skin; ②The expression of miR-199a-5p is downregulated in keloid and miR-199a-5p can affect the cell cycle and suppress proliferation of keloid fibroblasts.It indicateds that miR-199a-5p may be involved in regulating fibroblastic proliferation.%目的 探讨并筛选出瘢痕疙瘩相关微小RNAs(miRNAs),检测其对瘢痕疙瘩成纤维细胞(keloid fibroblasts,KF)增殖的影响.方法 收集手术切除瘢痕疙瘩及正常皮肤组织标本各8例,采用基因芯片检测瘢痕疙瘩与正常皮肤组织差异表达的miRNA

  4. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer

    International Nuclear Information System (INIS)

    Cathepsin B and L (CATB, CATL), urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 play an important role in colorectal cancer invasion. The tumor marker utility and prognostic relevance of these proteases have not been evaluated in the same experimental setting and compared with that of CEA and CA-19-9. Protease, CEA and CA 19-9 serum or plasma levels were determined in 56 patients with colorectal cancer, 25 patients with ulcerative colitis, 26 patients with colorectal adenomas and 35 tumor-free control patients. Protease, CEA, CA 19-9 levels have been determined by ELISA and electrochemiluminescence immunoassay, respectively; their sensitivity, specificity, diagnostic accuracy have been calculated and correlated with clinicopathological staging. The protease antigen levels were significantly higher in colorectal cancer compared with other groups. Sensitivity of PAI-1 (94%), CATB (82%), uPA (69%), CATL (41%) were higher than those of CEA or CA 19-9 (30% and 18%, respectively). PAI-1, CATB and uPA demonstrated a better accuracy than CEA or CA 19-9. A combination of PAI-1 with CATB or uPA exhibited the highest sensitivity value (98%). High CATB, PAI-1, CEA and CA 19-9 levels correlated with advanced Dukes stages. CATB (P = 0.0004), CATL (P = 0.02), PAI-1 (P = 0.01) and CA 19-9 (P = 0.004) had a significant prognostic impact. PAI-1 (P = 0.001), CATB (P = 0.04) and CA 19-9 (P = 0.02) proved as independent prognostic variables. At the time of clinical detection proteases are more sensitive indicators for colorectal cancer than the commonly used tumor markers. Determinations of CATB, CATL and PAI-1 have a major prognostic impact in patients with colorectal cancer

  5. Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System

    Directory of Open Access Journals (Sweden)

    Sayyed Morteza Hosseini

    2008-01-01

    Full Text Available Background: The aim of this study was to establish a cell-free sequential culture system that cansupport high levels of in vitro embryo development and blastocyst formation from bovine zygotes.To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTAon bovine zygote in vitro development.Materials and Methods: Bovine presumptive zygotes were derived from oocytes matured, andfertilized in vitro and cultured in synthetic oviductal fluid sequential medium in a two-steps manner;SOF 1 for the first 3 days and SOF 2 for the second 5-6 days of in vitro embryo development. Inorder to evaluate the effect of different modifications of the basic medium on embryo development,glucose was added to the second phase (SOF A, serum was added to the first phase (SOF C andEDTA alone (SOF D or in combination with serum (SOF E was added into the first phase of invitro embryo culture. The results of each composition were compared with each other and with theresults of embryo development in TCM199 vero cell co-culture system.Results: Glucose addition to the second phase of embryo culture, improved the developmentalcompetency; however, the differences were not significant. Serum addition to the first phase ofembryo culture, significantly improved the developmental competency of embryos beyond thecleavage stage, compared to all the treatment and TCM199 co-culture groups. EDTA supplementationof culture medium, either alone or in combination with serum, significantly inhibits the embryodevelopment beyond the morula stage.Conclusion: The results indicated that culture of bovine presumptive zygotes in two steps cell-freeculture system, can support embryo development, and addition of serum throughout the culture andglucose to the second step significantly increased overall developmental competency compared toTCM199 co-culture system.

  6. Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase

    International Nuclear Information System (INIS)

    Graphical abstract: - Highlights: • Three dimensional ordered macroporous magnetic electrode was newly used in electrochemical immunosensor. • The large surface area of macroporous magnetic electrode could improve the immobilized amount of antibody. • Au nanoparticles functionalized SBA-15 was used to immobilize enzyme labeled Ab2 and enzyme. • Macroporous magnetic electrode and Au nanoparticles composite facilitated the direct electron transfer of enzyme. • The immunoassay avoided adding electron transfer mediator, simplifying the procedure. - Abstract: A sandwich-type electrochemical immunosensor for the detection of carbohydrate antigen 19-9 (CA 19-9) antigen based on the immobilization of primary antibody (Ab1) on three dimensional ordered macroporous magnetic (3DOMM) electrode, and the direct electrochemistry of horseradish peroxidase (HRP) that was used as both the label of secondary antibody (Ab2) and the blocking reagent. The 3DOMM electrode was fabricated by introducing core–shell Au–SiO2@Fe3O4 nanospheres onto the surface of three dimensional ordered macroporous (3DOM) Au electrode via the application of an external magnet. Au nanoparticles functionalized SBA-15 (Au@SBA-15) was conjugated to the HRP labeled secondary antibody (HRP-Ab2) through the Au–SH or Au–NH3+ interaction, and HRP was also used as the block reagent. The formation of antigen–antibody complex made the combination of Au@SBA-15 and 3DOMM exhibit remarkable synergistic effects for accelerating direct electron transfer (DET) between HRP and the electrode. Under the optimal conditions, the DET current signal increased proportionally to CA 19-9 concentration in the range of 0.05 to 15.65 U mL−1 with a detection limit of 0.01 U mL−1. Moreover, the immunosensor showed high selectivity, good stability, satisfactory reproducibility and regeneration. Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained

  7. Amperometric carbohydrate antigen 19-9 immunosensor based on three dimensional ordered macroporous magnetic Au film coupling direct electrochemistry of horseradish peroxidase

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qi [College of Sciences, Nanjing Tech University, Nanjing 211816 (China); Chen, Xiaojun, E-mail: chenxj_njut@126.com [College of Sciences, Nanjing Tech University, Nanjing 211816 (China); State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009 (China); Tang, Yin [Zhangjiagang Hospital of Traditional Chinese Medicine, Zhangjiagang 215600 (China); Ge, Lingna; Guo, Buhua [College of Sciences, Nanjing Tech University, Nanjing 211816 (China); Yao, Cheng, E-mail: yaochengnjut@163.com [College of Sciences, Nanjing Tech University, Nanjing 211816 (China)

    2014-03-01

    Highlights: • Three dimensional ordered macroporous magnetic electrode was newly used in electrochemical immunosensor. • The large surface area of macroporous magnetic electrode could improve the immobilized amount of antibody. • Au nanoparticles functionalized SBA-15 was used to immobilize enzyme labeled Ab₂ and enzyme. • Macroporous magnetic electrode and Au nanoparticles composite facilitated the direct electron transfer of enzyme. • The immunoassay avoided adding electron transfer mediator, simplifying the procedure. Abstract: A sandwich-type electrochemical immunosensor for the detection of carbohydrate antigen 19-9 (CA 19-9) antigen based on the immobilization of primary antibody (Ab₁) on three dimensional ordered macroporous magnetic (3DOMM) electrode, and the direct electrochemistry of horseradish peroxidase (HRP) that was used as both the label of secondary antibody (Ab₂) and the blocking reagent. The 3DOMM electrode was fabricated by introducing core–shell Au–SiO₂@Fe₃O₄ nanospheres onto the surface of three dimensional ordered macroporous (3DOM) Au electrode via the application of an external magnet. Au nanoparticles functionalized SBA-15 (Au@SBA-15) was conjugated to the HRP labeled secondary antibody (HRP-Ab₂) through the Au–SH or Au–NH₃⁺ interaction, and HRP was also used as the block reagent. The formation of antigen–antibody complex made the combination of Au@SBA-15 and 3DOMM exhibit remarkable synergistic effects for accelerating direct electron transfer (DET) between HRP and the electrode. Under the optimal conditions, the DET current signal increased proportionally to CA 19-9 concentration in the range of 0.05 to 15.65 U mL⁻¹ with a detection limit of 0.01 U mL⁻¹. Moreover, the immunosensor showed high selectivity, good stability, satisfactory reproducibility and regeneration. Importantly, the developed method was used to assay clinical serum specimens, achieving a good relation with those obtained from

  8. Untersuchungen zur Funktion der MicroRNAs miR-199a/b-3p und miR-223 im kardiovaskulären System

    OpenAIRE

    Leierseder, Simon Johannes

    2016-01-01

    In MicroRNA-Expressionsanalysen an isolierten Herzzellen wurden miR-199a/b 3p und miR-223 als hochgradig in kardialen Fibroblasten gegenüber Kardiomyozyten angereicherte MicroRNAs identifiziert und deshalb für weiterführende Studien ausgewählt. Die Ergebnisse von Untersuchungen an kultivierten Fibroblasten und in Mausmodellen für kardialen Stress legen nahe, dass beide MicroRNAs wichtige Rollen im Herz übernehmen. Darüber hinaus zeigte die Untersuchung von Blutplättchen und Megakaryozyten, da...

  9. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

    Science.gov (United States)

    Yuan, Chuanshu; Yang, Kai; Tang, Hong; Chen, Dan

    2016-01-01

    Background At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SCCAg), ferritin, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and α-fetoprotein (AFP) for patients with oral/oropharyngeal squamous carcinoma (OSCC/OPSCC). Methods One hundred and sixty-nine cases of patients with OSCC/OPSCC as the experimental group, 86 cases of oral benign tumor patients as the control group, and 30 cases of healthy people as the normal control group were studied. The levels of serum Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP were measured using electrochemiluminescence immunoassay. Results The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in patients with OSCC/OPSCC were significantly higher than those of benign tumor and healthy control group (P0.05). The level of serum Cyfra21-1 in patients with early OSCC/OPSCC (stage I + II) was significantly higher than that of benign tumor and healthy control group (P0.05). The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in the middle-late stage of patients with OSCC/OPSCC (stage III + IV) were significantly higher than those of patients with the early OSCC/OPSCC, benign tumor, and healthy control group (P<0.05). The diagnostic cutoff levels of Cyfra21-1, SCCAg, ferritin, and CEA were 2.17, 0.72, 109.95, and 1.99 ng/mL, respectively. The sensitivities were 60.36%, 73.37%, 81.66%, and 66.27%, respectively. The specificities were 81.03%, 68.10%, 40.52%, and 61.21%, respectively. Conclusion Cyfra21-1, SCCAg, ferritin, and CEA had diagnostic values for patients with OSCC/OPSCC. Meanwhile, Cyfra21-1 had better early diagnostic value for patients with OSCC/OPSCC. PMID:27350753

  10. Catalytic residues Lys197 and Arg199 of Bacillus subtilis phosphoribosyl diphosphate synthase. Alanine-scanning mutagenesis of the flexible catalytic loop

    DEFF Research Database (Denmark)

    Hove-Jensen, Bjarne; Bentsen, Ann-Kristin K; Harlow, Kenneth W

    2005-01-01

    Eleven of the codons specifying the amino acids of the flexible catalytic loop [KRRPRPNVAEVM(197-208)] of Bacillus subtilis phosphoribosyl diphosphate synthase have been changed individually to specify alanine. The resulting variant enzyme forms, as well as the wildtype enzyme, were produced in an...... Escherichia coli strain lacking endogenous phosphoribosyl diphosphate synthase activity and purified to near homogeneity. The B. subtilis phosphoribosyl diphosphate synthase mutant variants K197A and R199A were studied in detail. The physical properties of the two enzymes were similar to those of the wildtype...

  11. Sensitivity and specificity of CA242 in gastro-intestinal cancer. A comparison with CEA, CA50 and CA 19-9.

    OpenAIRE

    Nilsson, O; Johansson, C.; Glimelius, B.; Persson, B.; Nørgaard-Pedersen, B.; Andrén-Sandberg, A.; Lindholm, L.

    1992-01-01

    A serological assay for the quantitative determination of the novel tumour-associated epitope CA242 was developed and used for determination of sensitivity and specificity of CA242 in gastrointestinal cancer. The CA242 assay showed a better tumour specificity than CA50 (and CA 19-9). This was most noticeable in benign hepatobiliary disease. The sensitivity at 90% specificity cut-off level was approximately three times higher for CA242 compared to CA50 in colo-rectal cancer Dukes A, B and C, w...

  12. Comparison of a new tumour marker CA 242 with CA 19-9, CA 50 and carcinoembryonic antigen (CEA) in digestive tract diseases.

    OpenAIRE

    Kuusela, P.; Haglund, C.; Roberts, P. J.

    1991-01-01

    The levels of CA 242, a new tumour marker of carbohydrate nature, were measured in sera of 185 patients with malignancies of the digestive tract and of 123 patients with benign digestive tract diseases. High percentages of elevated CA 242 levels (greater than 20 U ml-1) were recorded in patients with pancreatic and biliary cancers (68%). The sensitivity was somewhat lower than that of CA 19-9 (76%) and CA 50 (73%). On the other hand, in benign pancreatic and biliary tract diseases the CA 242 ...

  13. Evidence for {open_quotes}magnetic rotation{close_quotes} in nuclei: New results on the M1-bands of {sup 198,199}Pb

    Energy Technology Data Exchange (ETDEWEB)

    Clark, R.M. [Lawrence Berkeley National Lab., CA (United States)

    1996-12-31

    Lifetimes of states in four of the M1-bands in {sup 198,199}Pb have been determined through a Doppler Shift Attenuation Method measurement performed using the Gammasphere array. The deduced B(M1) values, which are a sensitive probe of the underlying mechanism for generating these sequences, show remarkable agreement with Tilted Axis Cranking (TAC) calculations. Evidence is also presented for the possible termination of the bands. The results represent clear evidence for a new concept in nuclear excitations: {open_quote}magnetic rotation{close_quote}.

  14. Intensified adjuvant therapy for pancreatic and periampullary adenocarcinoma: survival results and observations regarding patterns of failure, radiotherapy dose and CA19-9 levels

    International Nuclear Information System (INIS)

    Purpose: Primary endpoints were 1. To determine if, in the context of postoperative adjuvant therapy of pancreatic and nonpancreatic periampullary adenocarcinoma, continuous infusion (C.I.) 5-fluorouracil (5-FU) and leucovorin (Lv), combined with continuous-course external-beam radiotherapy (EBRT) to liver (23.4-27.0 Gy), regional lymph nodes (50.4-54.0 Gy) and tumor bed (50.4-57.6 Gy), followed by 4 months of C.I. 5-FU/Lv without EBRT could be given with acceptable toxicity. 2. To determine an estimate of disease-free and overall survival (DFS, OS) with this treatment in this context. Secondary endpoints were 1. To observe the effects of therapy at two different dose levels of irradiation, and 2. To observe for correlations among DFS, OS and CA 19-9 levels during therapy. Methods: Patients received C.I. 5-FU 200 mg/m2 and Lv 5 mg/m2 Monday through Friday during EBRT, and 4 cycles of the same chemotherapy without EBRT were planned for each 2 weeks of 4, beginning 1 month following the completion of EBRT. Therapy was to begin within 10 weeks of surgery and patients were monitored for disease recurrence, toxicity, and CA 19-9 levels before the start of EBRT/5-FU/Lv, before each cycle of C.I. 5-FU/Lv, and periodically after the completion of therapy. There were two EBRT dosage groups: Low EBRT, 23.4 Gy to the whole liver, 50.4 Gy to regional nodes and 50.4 Gy to the tumor bed; High EBRT, 27.0 Gy to the whole liver, 54.0 Gy to regional nodes, and 57.6 Gy to the tumor bed. Results: 29 patients were enrolled and treated (23 with pancreatic cancer, and 6 with nonpancreatic periampullary cancer). Of these, 18 had tumor sizes ≥ 3 cm and 23 had at least one histologically involved lymph node; 6 had histologically positive resection margins. Mean time to start of EBRT/5-FU/Lv was 53 ± 2 days following surgery. The first 18 patients were in the Low EBRT Group and the last 11 in the High EBRT Group. Toxicity was moderate and manageable, including a possible case of late

  15. Rigorous limits for hadronic and semi-leptonic CP-violating coupling constants from the electric dipole moment of $^{199}$Hg

    CERN Document Server

    Singh, Yashpal

    2014-01-01

    Relativistic many-body methods at different levels of approximations are employed to gain insights into the passage of the electron correlations from lower to higher levels in the accurate determination of the electric dipole polarizability ($\\alpha_d$) and electric dipole moment (EDM) due to the electron-nucleus tensor-pseudotensor (T-PT) and nuclear Schiff moment (NSM) interactions of the $^{199}$Hg ground state. Moreover, plausible reasons for the differences in the previous calculations are pointed out. Comparison between the calculated and experimental results of $\\alpha_d$ indicates that our EDM calculations are about 3\\% accurate which in combination with the measured value of $^{199}$Hg EDM yield limits for the T-PT coupling constant $C_T < 2.31 \\times 10^{-9}$ and the NSM $S<1.55 \\times 10^{-12}|e|fm^3$. From this limit of $S$ and the latest nuclear structure calculation, we get improved limits for the strong CP violating parameter $\\theta_{QCD} < 2.12 \\times 10^{-10}$ and the combined up an...

  16. Residues R{sup 199}H{sup 200} of prototype foamy virus transactivator Bel1 contribute to its binding with LTR and IP promoters but not its nuclear localization

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Qinglin; Tan, Juan [Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin 300071 (China); Cui, Xiaoxu [Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin 300071 (China); Centre Laboratory, TianJin 4th Centre Hospital, Tianjin 300140 (China); Luo, Di; Yu, Miao [Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin 300071 (China); Liang, Chen [Lady Davis Institute, Jewish General Hospital, Montreal, QC, Canada H3T 1E2 (Canada); Departments of Medicine McGill University, Montreal, QC (Canada); Microbiology and Immunology, McGill University, Montreal, QC (Canada); Qiao, Wentao, E-mail: wentaoqiao@nankai.edu.cn [Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin 300071 (China)

    2014-01-20

    Prototype foamy virus encodes a transactivator called Bel1 that enhances viral gene transcription and is essential for PFV replication. Nuclear localization of Bel1 has been reported to rely on two proximal basic motifs R{sup 199}H{sup 200} and R{sup 221}R{sup 222}R{sup 223} that likely function together as a bipartite nuclear localization signal. In this study, we report that mutating R{sup 221}R{sup 222}R{sup 223}, but not R{sup 199}H{sup 200}, relocates Bel1 from the nucleus to the cytoplasm, suggesting an essential role for R{sup 221}R{sup 222}R{sup 223} in the nuclear localization of Bel1. Although not affecting the nuclear localization of Bel1, mutating R{sup 199}H{sup 200} disables Bel1 from transactivating PFV promoters. Results of EMSA reveal that the R{sup 199}H{sup 200} residues are vital for the binding of Bel1 to viral promoter DNA. Moreover, mutating R{sup 199}H{sup 200} in Bel1 impairs PFV replication to a much greater extent than mutating R{sup 221}R{sup 222}R{sup 223}. Collectively, our findings suggest that R{sup 199}H{sup 200} directly participate in Bel1 binding to viral promoter DNA and are indispensible for Bel1 transactivation activity. - Highlights: • The R{sup 221}R{sup 222}R{sup 223} residues are essential for the nuclear localization of Bel1. • Although not affecting the nuclear localization of Bel1, mutating R{sup 199}H{sup 200} disables Bel1 from transactivating PFV promoters. • The R{sup 199}H{sup 200} residues directly participate in Bel1 binding to viral promoter DNA. • Mutating R{sup 199}H{sup 200} in Bel1 impairs PFV replication to a much greater extent than mutating R{sup 221}R{sup 222}R{sup 223}.

  17. Comparison and harmonization assessment of mutual among four different immunoassay systems in CA19-9 test%CA19-9测定在4个免疫检测系统的差异及互认能力的评估

    Institute of Scientific and Technical Information of China (English)

    宋超; 张曙云; 周俊; 单志明; 周永列

    2013-01-01

    目的 研究4个免疫检测系统对CA19-9检测的差异和一致性,探讨实现CA19-9检测结果互认的基础.方法 检测系统比对研究.汇集整理CA19-9室间质量评价活动批号的数据(卫生部200811 ~ 201215,浙江省080309 ~ 120211);搜集120份不同CA19-9浓度的新鲜血样本;搜集2010年9月至2012年3月不同检测系统非肿瘤体检人群实测CA19-9数据.选择4个稳定的检测系统Abbott Architect i2000、Roche E170、Beckman Dxi800和Siemens CentaurXP作为研究对象,统计分析在卫生部和浙江省室间质评(冻干粉和液体血清)中的组间差异;参照CLSI EP9-A2进行120份新鲜血样本的比对分析;计算各检测系统实测CA19-9的95%置信区间并分析.采用散点图、加权回归方程进行统计比较.结果 室间质评与新鲜血比对出现颠倒的结果.室间质评均表现出检测系统组间较大的偏倚,斜率bw在1.340到4.683之间,相关系数r较好;新鲜血比对中r不理想,但斜率bw接近于1.新鲜血比对中在推荐浓度27 U/ml时,检测系统两两间偏移分别为Abbott-Roche(-6.41%),Beckman-Roche(-5.07%),Siemens-Roche(13.15%),Beckman-Abbott(2.46%),Siemens-Abbott(22.52%),Siemens-Beckman(39.66%).部分检测系统间实测95%置信区间差异有统计学意义.结论 在研究检测系统差异时,在较低浓度CA19-9的结果在4个检测系统间具有互认的基础,但在高浓度时,检测系统间的差异明显增大,风险较高,尚无法实现互认.%Objective To investigate the differences and harmonization of immunoassay systems in detecting CA19-9 and to assess the possibility of mutual recognition in different laboratories.Methods Data were collected and analyzed from External Quality Assessments (EQA) of NCCL(Lots:200811-201215) and ZJCCL(Lots:080309-120211).120 fresh serum with different concentrations of CA19-9 were collected.The CA19-9 results of healthy people were also collected from September 2010 to March

  18. Measurement of delta(1)J((199)Hg, (31)P) in [HgPCy3(OAc)2]2 and relativistic ZORA DFT investigations of mercury-phosphorus coupling tensors.

    Science.gov (United States)

    Bryce, David L; Courchesne, Noémie Manuelle Dorval; Perras, Frédéric A

    2009-12-01

    Using 31P solid-state NMR spectroscopy, anisotropy in the indirect 199Hg-31P spin-spin coupling tensor (DeltaJ) for powdered [HgPCy3(OAc)2]2 (1) has been measured as 4700 +/- 300 Hz. Zeroth-order regular approximation (ZORA) density functional theory (DFT) calculations, including scalar and spin-orbit relativistic effects, performed on 1 and a series of other related compounds show that DeltaJ(199Hg, (31)P) arises entirely from the ZORA Fermi-contact-spin-dipolar cross term. The calculations validate assumptions made in the spectral analysis of 1 and in previous determinations of DeltaJ in powder samples, namely that J is axially symmetric and shares its principal axis system with the direct dipolar coupling tensor (D). Agreement between experiment and theory for various 199Hg, 31P spin-spin coupling anisotropies is reasonable; however, experimental values of 1J(199Hg, 31P)(iso) are significantly underestimated by the calculations. The most important improvements in the agreement were obtained as a result of including more of the crystal lattice in the model used for the calculations, e.g., a change of 43% was noted for 1J(199Hg, 31P)(iso) in [HgPPh3(NO3)2]2 depending on whether the two or three nearest nitrate ions are included in the model. Finally, we have written a computer program to simulate the effects of non-axial symmetry in J and of non-coincidence of the J and D on powder NMR spectra. Simulations clearly show that both of these effects have a pronounced impact on the 31P NMR spectrum of 199Hg-31P spin pairs, suggesting that the effects should be observable experimentally if a suitable compound can be identified. PMID:20056396

  19. Spin excitations in a K0.84Fe1.99Se2 superconductor as studied by M(o)ssbauer spectroscopy

    Institute of Scientific and Technical Information of China (English)

    Li Zhi-Wei; Ma Xiao-Ming; Pang Hua; Li Fa-Shen

    2012-01-01

    M(o)ssbauer spectroscopy was used to probe the site-specific information of a K0.84Fe1.99Se2 superconductor.A spin excitation gap,△E ≈ 5.5 meV,is observed by analyzing the temperature dependence of the hyperfine magnetic field (HMF) at the iron site within the spin wave theory. Using the simple model suggested in the literature,the temperature dependence of the HMF is well reproduced,suggesting that,below room temperature,the alkali metal intercalated iron-selenide superconductors can be regarded as ferromagnetically coupled spin blocks that interact with each other antiferromagnetically to form the observed checkerboard-like magnetic structure.

  20. Study of the k+ to pi+, neutrino, anti-neutrino decay in the momentum region p(pi+) < 199 MeV/C

    Energy Technology Data Exchange (ETDEWEB)

    Artamonov, A.V.; Bassalleck, B.; Bhuyan, B.; Blackmore, E.W.; Bryman, D.A.; Chen, S.; Chiang, I.-H.; Christidi, I.-A.; Cooper, P.S.; Diwan, M.V.; Frank, J.S.; /Serpukhov, IHEP /New Mexico U. /Brookhaven /TRIUMF /British Columbia U. /Tsinghua U., Beijing /SUNY, Stony Brook /Fermilab /Kyoto U. /KEK, Tsukuba /Moscow, INR

    2009-01-01

    Experiment E949 at Brookhaven National Laboratory has observed three candidate events for the decay K{sup +} {yields} {pi}{sup +}{nu}{bar {nu}} in the pion momentum region 140 < P{sub {pi}} < 199 MeV/c in a exposure of 1.71 x 10{sup 12} stopped kaons with an estimated total background of 0.93 {+-} 0.17(stat.){sub -0.24}{sup +0.32}(syst.) events. Combination of this observation with previous results, assuming a pion spectrum as predicted by the standard model, produces a branching fraction of {beta} = (1.73{sub -1.05}{sup +1.15}) x 10{sup -10}. We also give the interpretation of the combined results for alternative model of the decay K{sup +} {yields} {pi}{sup +} + nothing.

  1. Allelic imbalance and cytogenetic deletion of 1p in colorectal adenomas: a target region identified between DIS199 and DIS234

    DEFF Research Database (Denmark)

    Bomme, L; Heim, S; Bardi, G;

    1998-01-01

    Both cytogenetic and molecular genetic analyses have shown that many colorectal adenomas carry an acquired deletion distally in the short arm of one chromosome 1, but the two methods have never been brought to bear on the same tumors. The major part of this study was the analysis of 53 previously...... short-term cultured and karyotyped colorectal adenomas for allelic imbalance at eight microsatellite loci in 1p. Allelic imbalances were detected in seven of the 12 adenomas that had cytogenetically visible abnormalities of chromosome 1, as well as in four adenomas that either had a normal karyotype...... (one case) or had clonal chromosome abnormalities that did not seem to involve chromosome 1 (three cases); i.e., 30% of the adenomas had abnormalities involving 1p by the combined approach. A minimal region of overlap seemed to map to between DIS199 and DIS234, suggesting that this is a relevant target...

  2. 血清肿瘤标志物CA125、CA199、CEA、NSE联合检测在肺癌诊断中的应用%Serum tumor marker CA125, CA199, CEA, NSE combined detection of lung cancer diagnosis

    Institute of Scientific and Technical Information of China (English)

    王峰

    2010-01-01

    目的 探讨血清肿瘤标志物CA125、CA199、CEA、NSE联合检测在肺癌诊断中的应用价值.方法 选择本院2009年1月至2010年2月住院的肺癌患者220例肺癌病例组,选取同期我院健康体检人员为正常对照组250例,进行肿瘤标志物CA125、CA199、CEA、NSE进行联合检测.结果 经统计学分析肺癌病例组CA125、CEA、NSE血清水平明显高于正常对照组,差异有显著性意义(P<0.01),CA199在两组的表达水平比较无显著性差异(P>0.05).经统计学分析CA125、CEA、NSE与联合检测三项肿瘤标志物之间的敏感性、特异性比较有显著型差异(P<0.01).结论 单项肿瘤标志物检测,可以对临床有一定的指导性,但在敏感性、特异性均方面各有欠缺,很多肿瘤标志物与其他相应的疾病有一定的交叉性,对单项肿瘤标志物检测指导临床诊断价值有限,同时选用多项肿瘤标志物进行检测,可以达到单项检测在敏感性和特异性上的不足,能够有效的为临床提供有价值的佐证资料,以能够达到初筛和早期诊断的目的 .%Objective To explore the serum tumor marker CA125, CA199,CEA,NSE combined detection in the diagnosis of lung cancer in the application. Methods The hospital in January 2009-2010 year in February hospitalized patients with lung cancer group of 220 cases of lung cancer cases, select the same period the health physical examination in our hospital staff to the normal control group of 250 patients for tumor markers CA125,CA199,CEA,NSE to conduct joint detection. Results After statistical analysis of lung cancer cases group of CA125, CEA, NSE serum levels were significantly higher than the normal control group, the difference was significant(P < 0. 01 ), CA199 expression levels in the two groups showed no significant difference (P >0. 05 ). The statistical analysis of CA125, CEA, NSE and the combined detection of three tumor markers between the sensitivity,specificity,and there

  3. 布加综合征肿瘤相关血清学检查的临床意义%Clinical significances of AFP,CA199 and CA125 levels in Budd Chiari syndrome

    Institute of Scientific and Technical Information of China (English)

    许伟; 徐浩; 祖茂衡; 顾玉明; 邹文卫; 张庆桥; 魏宁

    2013-01-01

    Objective To explore the clinical significances of serum AFP,CA199 and CA125 levels in patients with Budd-Chiari syndrome(BCS). Methods A retrospective analysis of serum AFP,CA125 ,CA1 99 levels was carried out in 188 patients with BCS,in order to analyze the differences between subtypes of the disease. The scrum CA125,CA199 levels of 188 patients were compared with hepatic cirrhosis and healthy people,so as to evaluate the value of CA125,CA199 in the diagnosis of BCS. Results For the levels of serum CA125 and CA199,there was significant difference between patients with hepatic cirrhosis , BCS and healthy control group (P<0. 05) ; for the level of serum CA199, there was significant different between patients with hepatic cirrhosis and BCS( P<0. 01); there was no significant difference between patients with hepatic cirrhosis and BCS for the level of serum CA125. There was no significant difference between subtypes of BCS for the levels of serum CA125 ,CA1 99. CA199 was useful in judging hepatic cirrhosis and BCS, cutoff point was 31. 14 U/mL,and the sensitivity,specificity were 64 % ,85. o% , respectively. The standard of AFP in BCS was (3. 96 ± 2. 45)μg/L( 95%CI 0 - 8. 76μg/L) , between the overall standard (0 - 20 μg/L) of normal population. The standards of 16 BCS patients with hcpatocarcinoma:4 cases were greater than 1000μg/L,6 cases were in 20 - 400μg/L,7 cases were less than 20μg/ L. Conclusion The mean AFP standard of BCS is in the normal range. The amount of CA125,CA199 is greatly related to evaluate the degree of liver injury and judged the progress of BCS.%目的 探讨布加综合征(BCS)肿瘤相关血清学检查如AFP、CA125、CA199变化的临床意义.方法 回顾分析188例BCS肿瘤相关血清AFP、CA125、CA199水平,分析BCS不同亚型之间的差异,并与肝硬化血清、正常体检人群AFP、CA125、CA199水平对照.ROC分析评价CA199、CA125对BCS、肝硬化的诊断价值.结果 BCS组、肝硬化组血清CA125、CA199

  4. Physical and underway data collected aboard the KNORR during cruise KN199-02 in the Davis Strait from 2010-09-07 to 2010-09-29 (NODC Accession 0104295)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC accession 0104295 includes physical and underway data collected aboard the KNORR during cruise KN199-02 in the Davis Strait from 2010-09-07 to 2010-09-29....

  5. Effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer

    Institute of Scientific and Technical Information of China (English)

    Su-Yu Zhu; Jie Tan; Chen-Lu Zhang; Qun-Ying Wu; Xue-Xin Xie; Hua-Fang Yin

    2016-01-01

    Objective:To study the effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer.Methods:A total of 80 patients with advanced ovarian cancer in our hospital from December 2012 to December 2015 were enrolled in this study. The subjects were divided into control group (n=40) and experiment group (n=40) randomly. The control group were treated with paclitaxel and cisplatin, the experiment group were treated with paclitaxel liposome combined with nedaplatin. 21 days for a period of treatment and the two groups were treated for 3 periods. The serum HE4, CA125, CA19-9, AFP, CEA levels and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before and after treatment were compared. Results:There were no significantly differences of the serum HE4, CA125, CA19-9, AFP, CEA level and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before treatment (P>0.05). The serum HE4, CA125, CA19-9, AFP and CEA level of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly lower than control group (P<0.05). The peripheral blood CD3+, CD4+, CD8+and NK cells of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly higher than control group (P<0.05). Conclusions:Paclitaxel liposome combined with nedaplatin can significantly reduce the serum HE4, CA125, CA19-9, AFP and CEA levels, improve peripheral blood CD3+, CD4+, CD8+ and NK levels of patients with advanced ovarian cancer, and it was worthy clinical application.

  6. Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199.

    Directory of Open Access Journals (Sweden)

    Carolina Paola García

    Full Text Available Human embryonic stem cells (hESCs are hypersensitive to genotoxic stress and display lower survival ability relative to their differentiated progeny. Herein, we attempted to investigate the source of this difference by comparing the DNA damage responses triggered by the topoisomerase I inhibitor camptothecin, in hESCs, human induced pluripotent stem cells (hiPSCs and hESCs-derived neuroprogenitors (NP. We observed that upon camptothecin exposure pluripotent stem cells underwent apoptosis more swiftly and at a higher rate than differentiated cells. However, the cellular response encompassing ataxia-telangiectasia mutated kinase activation and p53 phosphorylation both on serine 15 as well as on serine 46 resulted very similar among the aforementioned cell types. Importantly, we observed that hESCs and hiPSCs express lower levels of the anti-apoptotic protein Bcl-2 than NP. To assess whether Bcl-2 abundance could account for this differential response we treated cells with ABT-263, WEHI-539 and ABT-199, small molecules that preferentially target the BH3-binding pocket of Bcl-xL and/or Bcl-2 and reduce their ability to sequester pro-apoptotic factors. We found that in the absence of stress stimuli, NP exhibited a higher sensitivity to ABT- 263 and WEHI-539 than hESCs and hiPSCs. Conversely, all tested cell types appeared to be highly resistant to the Bcl-2 specific inhibitor, ABT-199. However, in all cases we determined that ABT-263 or WEHI-539 treatment exacerbated camptothecin-induced apoptosis. Importantly, similar responses were observed after siRNA-mediated down-regulation of Bcl-xL or Bcl-2. Taken together, our results suggest that Bcl-xL contrary to Bcl-2 contributes to ensure cell survival and also functions as a primary suppressor of DNA double-strand brake induced apoptosis both in pluripotent and derived NP cells. The emerging knowledge of the relative dependence of pluripotent and progenitor cells on Bcl-2 and Bcl-xL activities may help

  7. Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199

    Science.gov (United States)

    Dimopoulos, Nicolás Alexis; Fernandez Espinosa, Damián Darío; Miriuka, Santiago Gabriel; Sevlever, Gustavo Emilio; Romorini, Leonardo; Scassa, María Elida

    2016-01-01

    Human embryonic stem cells (hESCs) are hypersensitive to genotoxic stress and display lower survival ability relative to their differentiated progeny. Herein, we attempted to investigate the source of this difference by comparing the DNA damage responses triggered by the topoisomerase I inhibitor camptothecin, in hESCs, human induced pluripotent stem cells (hiPSCs) and hESCs-derived neuroprogenitors (NP). We observed that upon camptothecin exposure pluripotent stem cells underwent apoptosis more swiftly and at a higher rate than differentiated cells. However, the cellular response encompassing ataxia-telangiectasia mutated kinase activation and p53 phosphorylation both on serine 15 as well as on serine 46 resulted very similar among the aforementioned cell types. Importantly, we observed that hESCs and hiPSCs express lower levels of the anti-apoptotic protein Bcl-2 than NP. To assess whether Bcl-2 abundance could account for this differential response we treated cells with ABT-263, WEHI-539 and ABT-199, small molecules that preferentially target the BH3-binding pocket of Bcl-xL and/or Bcl-2 and reduce their ability to sequester pro-apoptotic factors. We found that in the absence of stress stimuli, NP exhibited a higher sensitivity to ABT- 263 and WEHI-539 than hESCs and hiPSCs. Conversely, all tested cell types appeared to be highly resistant to the Bcl-2 specific inhibitor, ABT-199. However, in all cases we determined that ABT-263 or WEHI-539 treatment exacerbated camptothecin-induced apoptosis. Importantly, similar responses were observed after siRNA-mediated down-regulation of Bcl-xL or Bcl-2. Taken together, our results suggest that Bcl-xL contrary to Bcl-2 contributes to ensure cell survival and also functions as a primary suppressor of DNA double-strand brake induced apoptosis both in pluripotent and derived NP cells. The emerging knowledge of the relative dependence of pluripotent and progenitor cells on Bcl-2 and Bcl-xL activities may help to predict

  8. $^{204m}$Pb: A new Probe for TDPAC Experiments in Biology Complementing the Well Established Probes $^{111}$Cd and $^{199m}$Hg

    CERN Multimedia

    2002-01-01

    The short-lived nuclear probes $\\,^{111m}$Cd( t$_{1/2}$ = 49 min) , $^{199m}$Hg ( t$_{1/2}$ = 43 min) , and $^{204m}$Pb( t$_{1/2}$ = 43 min) supplied by ISOLDE are used to study the interaction of metals with biological macromolecules like, e.g., DNA and proteins. The structure and dynamics of metal sites in biomolecules are important in determining the functional efficiency of these macromolecules. Many life processes are based on such interactions. In order to study those metal sites close to physiological conditions a highly sensitive spectroscopic method is required, like Time Differential Perturbed Angular Correlation (TDPAC). Here, a radioactive atom is placed at the site of interest and by correlating the emitted $\\gamma$-quanta in space and on a nanosecond time scale local structural information is provided via the Nuclear Quadrupole Interaction. These investigations will allow a deeper insight into the adaptivity and rigidity of metal sites in the blue copper proteins (electron transfer proteins), th...

  9. A giant Ly$\\alpha$ nebula in the core of an X-ray cluster at $z=1.99$: implications for early energy injection

    CERN Document Server

    Valentino, F; Finoguenov, A; Strazzullo, V; Brun, A M C Le; Vignali, C; Bournaud, F; Dickinson, M; Renzini, A; Béthermin, M; Zanella, A; Gobat, R; Cimatti, A; Elbaz, D; Onodera, M; Pannella, M; Sargent, M T; Arimoto, N; Carollo, M; Starck, J-L

    2016-01-01

    We present the discovery of a giant $\\gtrsim$100 kpc Ly$\\alpha$ nebula detected in the core of the X-ray emitting cluster CL J1449+0856 at $z=1.99$ through Keck/LRIS narrow-band imaging. This detection extends the known relation between Ly$\\alpha$ nebulae and overdense regions of the Universe to the dense core of a $5-7\\times10^{13}$ M$_{\\odot}$ cluster. The most plausible candidates to power the nebula are two Chandra-detected AGN host cluster members. Given the physical conditions of the Ly$\\alpha$-emitting gas and the possible interplay with the X-ray phase, we argue that the Ly$\\alpha$ nebula would be short-lived ($\\lesssim10$ Myr) if not continuously replenished with cold gas at a rate of $\\gtrsim1000$ Myr. Cooling from the X-ray phase is disfavored as the replenishing mechanism, primarily because of the high Ly$\\alpha$ to X-ray luminosity ratio ($L_{\\mathrm{Ly\\alpha}}/L_{\\mathrm{X}} \\approx0.3$), $\\gtrsim10-1000\\times$ higher than in local cool-core clusters. Cosmological cold flows are disfavored by cu...

  10. Identification of IGFBP2 and IGFBP3 As Compensatory Biomarkers for CA19-9 in Early-Stage Pancreatic Cancer Using a Combination of Antibody-Based and LC-MS/MS-Based Proteomics.

    Science.gov (United States)

    Yoneyama, Toshihiro; Ohtsuki, Sumio; Honda, Kazufumi; Kobayashi, Makoto; Iwasaki, Motoki; Uchida, Yasuo; Okusaka, Takuji; Nakamori, Shoji; Shimahara, Masashi; Ueno, Takaaki; Tsuchida, Akihiko; Sata, Naohiro; Ioka, Tatsuya; Yasunami, Yohichi; Kosuge, Tomoo; Kaneda, Takashi; Kato, Takao; Yagihara, Kazuhiro; Fujita, Shigeyuki; Huang, Wilber; Yamada, Tesshi; Tachikawa, Masanori; Terasaki, Tetsuya

    2016-01-01

    Pancreatic cancer is one of the most lethal tumors, and reliable detection of early-stage pancreatic cancer and risk diseases for pancreatic cancer is essential to improve the prognosis. As 260 genes were previously reported to be upregulated in invasive ductal adenocarcinoma of pancreas (IDACP) cells, quantification of the corresponding proteins in plasma might be useful for IDACP diagnosis. Therefore, the purpose of the present study was to identify plasma biomarkers for early detection of IDACP by using two proteomics strategies: antibody-based proteomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics. Among the 260 genes, we focused on 130 encoded proteins with known function for which antibodies were available. Twenty-three proteins showed values of the area under the curve (AUC) of more than 0.8 in receiver operating characteristic (ROC) analysis of reverse-phase protein array (RPPA) data of IDACP patients compared with healthy controls, and these proteins were selected as biomarker candidates. We then used our high-throughput selected reaction monitoring or multiple reaction monitoring (SRM/MRM) methodology, together with an automated sample preparation system, micro LC and auto analysis system, to quantify these candidate proteins in plasma from healthy controls and IDACP patients on a large scale. The results revealed that insulin-like growth factor-binding protein (IGFBP)2 and IGFBP3 have the ability to discriminate IDACP patients at an early stage from healthy controls, and IGFBP2 appeared to be increased in risk diseases of pancreatic malignancy, such as intraductal papillary mucinous neoplasms (IPMNs). Furthermore, diagnosis of IDACP using the combination of carbohydrate antigen 19-9 (CA19-9), IGFBP2 and IGFBP3 is significantly more effective than CA19-9 alone. This suggests that IGFBP2 and IGFBP3 may serve as compensatory biomarkers for CA19-9. Early diagnosis with this marker combination may improve the prognosis of

  11. Paleomagnetism of ODP Leg 199 sediments: Implications for Paleogene and Neogene magnetic stratigraphy and paleolatitudes (abstract of paper presented at AGU Fall Meeting, San Francisco, 6-10 Dec 2002)

    OpenAIRE

    Parés, J.M.; Lanci, L.; Lyle, M.; Wilson, P. A.; Janecek, T.R.; J. Backman; Busch, W.H.; Coxall, H. K.; Faul, K.; Gaillot, P.; Hovan, S A; Knoop, P.; Kruse, S.; Lear, C.H.; Moore, T. C.

    2002-01-01

    ODP Leg 199 was designated to collect sediments along a latitudinal transect in the Pacific Ocean to better understand Paleogene sedimentation patterns and the system of equatorial currents. At ODP Sites 1218 through 1220, the magnetic record of the Paleogene Equatorial sediments extends back to Polarity Chron C20r (Middle Eocene), providing an unprecedented record of Paleogene magnetostratigraphy in Equatorial Pacific sediments. Paleomagnetic data were acquired on the JOIDES-Resolution pass-...

  12. ZZ KASHIL-E70, 199 N, 42 Photon Groups Cross Sections in MATXS Format Based on ENDF/B-VII.0 for Shielding Applications

    International Nuclear Information System (INIS)

    1 - Description: Format: MATXS, 204 nuclides processed with NJOY99.245. Number of groups: 199 neutron-, 42 photon-groups. 204 Nuclides including 8 thermal scattering law data: H-1, H-2, H-3, He-3, He-4, Li-6, Li-7, Be-9, Be-9, Be-9, B-10, B-11, C-nat, C-nat, N-14, N-15, O-16, O-17, F-19, Na-23, Mg-24, Mg-25, Mg-26, Al-27, Si-28, Si-29, Si-30, P-31, S-32, S-33, S-34, S-36, Cl-35, Cl-37, K-39, K-40, K-41, Ca-40, Ca-42, Ca-43, Ca-44, Ca-46, Ca-48, Sc-45, Ti-46, Ti-47, Ti-48, Ti-49, Ti-50, V-nat, Cr-50, Cr-52, Cr-53, Cr-54, Mn-55, Fe-54, Fe-56, Fe-57, Fe-58, Co-59, Ni-58, Ni-60, Ni-61, Ni-62, Ni-64, Cu-63, Cu-65, Ga-69, Ga-71, Y-89, Zr-90, Zr-91, Zr-92, Zr-94, Zr-96, Nb-93, Mo-92, Mo-94, Mo-95, Mo-96, Mo-97, Mo-98, Mo-100, Pd-102, Pd-104, Pd-105, Pd-106, Pd-108, Pd-110, Ag-107, Ag-109, Cd-106, Cd-108, Cd-110, Cd-112, Cd-113, Cd-114, Cd-116, In-113, In-115, I-127, Xe-124, Xe-126, Xe-128, Xe-129, Xe-130, Xe-131, Xe-132, Xe-134, Xe-136, Cs-133, Ba-138, Pr-141, Nd-143, Nd-145, Nd-146, Nd-148, Nd-150, Pm-147, Sm-147, Sm-151, Sm-152, Eu-151, Eu-152, Eu-153, Eu-154, Eu-155, Gd-152, Gd-154, Gd-155, Gd-156, Gd-157, Gd-158, Gd-160, Dy-164, Ho-165, Lu-175, Lu-176, Hf-174, Hf-176, Hf-177, Hf-178, Hf-179, Hf-180, Ta-181, Ta-182, W-182, W-183, W-184, W-186, Re-185, Re-187, Ir-191, Ir-193, Au-197, Pb-206, Pb-207, Pb-208, Bi-209, Th-230, Th-232, Pa-231, Pa-233, U-232, U-233, U-234, U-235, U-236, U-237, U-238, Np-237, Np-238, Np-239, Pu-236, Pu-237, Pu-238, Pu-239, Pu-240, Pu-241, Pu-242, Pu-243, Pu-244, Am-241, Am-242, Am-242m, Am-243, Cm-241, Cm-242, Cm-243, Cm-244, Cm-245, Cm-246, Cm-247, Cm-248, Bk-249, Cf-249, Cf-250, Cf-251, Cf-252, Cf-253, Es-253. Origin: ENDF/B-VII.0. Weighting spectrum: 300, 600, 1000, 2100 K. The KASHIL-E70 is a MATXS-format, 199-group neutron and 42-group photon cross section library for shielding applications based on ENDF/B-VII.0. The library contains 204 nuclide data including 8 thermal scattering law data processed by the NJOY99.259 code patched with NEA

  13. Histomorfologia do gânglio de Gasser, da rete mirabile carotídea e da hipófise de bovinos: estudo de 199 casos Histomorphology of the Gasserian ganglion, carotid rete mirabile and pituitary gland in cattle: a study of 199 cases

    Directory of Open Access Journals (Sweden)

    Raquel R. Rech

    2006-06-01

    Full Text Available O complexo heterogêneo de tecidos formado pelo gânglio de Gasser, rete mirabile carotídea e hipófise (GRH de 199 bovinos foram macro e microscopicamente avaliados. Vinte e um GRH eram de casos confirmados de raiva, 19 com diagnóstico de meningoencefalite por herpesvírus bovino-5 (BoHV-5, 11 casos de febre catarral maligna (FCM, 7 tinham abscesso de pituitária, 17 apresentaram lesões de encefalopatia hepática (status spongiosus atribuído à fibrose crônica por intoxicação por Senecio sp; e os 124 GRH restantes eram provenientes de bovinos que não apresentaram sinais neurológicos e que morreram ou foram eutanasiados por causas diversas. Nenhuma alteração histológica significativa foi observada no GRH dos 124 bovinos que não tinham sinais neurológicos, nos 17 bovinos com encefalopatia hepática, nem nos 19 bovinos afetados por meningoencefalite por BoHV-5. Alterações inflamatórias foram encontradas em 20 dos 21 casos de raiva e consistiam de proliferação de células satélites, nódulos gliais e infiltrado linfo-plasmocitário; alterações degenerativas incluíam cromatólise central, vacuolização neuronal e necrose neuronal com neuronofagia. Corpúsculos de inclusão eosinofílicos intracitoplasmáticos (de Negri foram encontrados em 19 dos 21 casos de raiva; neurite do nervo trigêmeo em 11 casos e neuroipofisite em 4 casos. O complexo GRH de 9 de 11 casos de FCM apresentava arterite necrosante, que afetava as artérias da rete mirabile carotídea. Em 7 dos 199 GRH examinados havia abscessos de pituitária caracterizados por agregados de neutrófilos e detritos celulares circundados por infiltrado mononuclear e cápsula fibrosa. Vários achados incidentais foram observados nos 199 GRH examinados histologicamente e não foram correlacionados com alterações ligadas a doenças. Estes incluíam cistos na cavidade hipofisária, agregados de fibrina e neutrófilos no seio cavernoso da rete mirabile carotídea, leve aumento

  14. ZZ MATJEF22.BOLIB, JEF-2.2 Multigroup Coupled (199 n + 42 gamma) X-Section Library in MATXS Format for Nuclear Fission Applications

    International Nuclear Information System (INIS)

    1 - Description of program or function: MATJEF22.BOLIB /1/ is a multigroup coupled (199 neutron groups + 42 photon groups) pseudo-problem-independent cross section library in MATXS format for nuclear fission applications. MATJEF22.BOLIB is based on the JEF-2.2 /2/ European nuclear data file and it was processed, through the NJOY /3/ system, in the VITAMIN-B6 /4/ group structure using the same parameters and calculation procedures. Original nuclear data file: JEF-2.2. Data processing systems: NJOY-94.66. Format: MATXS. Number of groups: 199 neutron groups and 42 photon groups. Thermal neutron groups: 36 groups below 5.043 eV with up-scatter cross sections. Neutron energy range: 1.0 E-5 eV - 19.64 MeV. Photon energy range: 1.0 keV - 30.0 MeV. Temperatures [K]: 300, 600, 1000, and 2100 (same values as in VITAMIN-B6). Background cross sections (Sigma-zeros) [barns]: 1, 10, 50, 100, 300, 1.0 E+3, 1.0 E+4, 1.0 E+5, 1.0 E+6, 1.0 E+10 (infinite dilution) (same values as in VITAMIN-B6). Legendre order: P7 for materials with Z ≤ 29 (copper); P5 for the remainders. Number of materials: 138 (list included). Neutron weighting spectrum: Corresponding to the IWT=4 input option in the GROUPR module of NJOY: from 1.0 E-5 eV to 0.125 eV → Maxwellian thermal spectrum (kT = 0.025 eV); from 0.125 eV to 820.8 keV → '1/E' slowing-down spectrum ; from 820.8 keV to 19.64 MeV → fission spectrum (fission temperature = 1.273 MeV). Photon weighting spectrum: Corresponding to the IWT=3 input option in the GAMINR module of NJOY: '1/E' spectrum with a 'roll-off' at lower energies to represent photoelectric absorption and a similar 'drop-off' at higher energies corresponding to the Q-value for neutron capture. The prompt neutron fission spectra for U-235, U-238 and Pu-239 are included in tabulated form in the package together with the neutron and photon group energy boundaries, the neutron and photon group lethargy boundaries and the neutron and photon group lethargy widths. The neutron

  15. Deregulation of miR-100, miR-99a and miR-199b in tissues and plasma coexists with increased expression of mTOR kinase in endometrioid endometrial carcinoma

    International Nuclear Information System (INIS)

    Alterations of mTOR gene expression have been implicated in the pathogenesis of endometrioid endometrial cancer however only few studies explored the cause of increased mTOR activation in this malignancy. miRNAs are small, noncoding RNAs, which were proven to regulated gene expression at the posttranscriptional level. The study aimed to explore deregulation of miRNAs targeting mTOR kinase (miR-99a, miR-100 and miR-199b) as a possible cause of its altered expression in EEC tissues. In addition expression of the three miRNAs was investigated in plasma of EEC patients and was assessed in terms of diagnostic and prognostic utility. We investigated expression of mTOR kinase transcripts in 46 fresh tissue samples. Expression of miR-99a, miR-100 and miR-199b was investigated in the same group of fresh samples, and in additional 58 FFPE sections as well as in 48 plasma samples using qPCR. Relative quantification was performed using experimentally validated endogenous controls. mTOR kinase expression was increased in EEC tissues and was accompanied by decreased expression of all three miRNAs. Down-regulation of the investigated miRNAs was discovered in plasma of EEC patients and miRNA signatures classified EEC tissues (miR-99a/miR-100/miR-199b) and plasma (miR-99a/miR-199b) samples with higher accuracy in comparison to single miRNAs. We also revealed that miR-100 was an independent prognostic marker of overall survival. We conclude that increased expression of mTOR kinase coexists with down-regulation of its targeting miRNAs, which could suggest a new mechanism of mTOR pathway alterations in EEC. In addition, our findings implicate that miRNA signatures can be considered promising biomarkers for early detection and prognosis of endometrioid endometrial carcinoma

  16. Pb(II) and Hg(II) binding to $\\textit{de novo}$ designed proteins studied by $^{204m}$Pb- and $^{199m}$Hg-Perturbed Angular Correlation of $\\gamma$-rays (PAC) spectroscopy : Clues to heavy metal toxicity

    CERN Multimedia

    2002-01-01

    $\\textit{De novo}$ design of proteins combined with PAC spectroscopy offers a unique and powerful approach to the study of fundamental chemistry of heavy metal-protein interactions, and thus of the mechanisms underlying heavy metal toxicity. In this project we focus on Pb(II) and Hg(II) binding to designed three stranded coiled coil proteins with one or two binding sites, mimicking a variety of naturally occurring thiolate-rich metal ion binding sites in proteins. The $^{204m}$Pb- and $^{199m}$Hg-PAC experiments will complement data already recorded with EXAFS, NMR, UV-Vis and CD spectroscopies.

  17. Antibody activity in ankylosing spondylitis sera to two sites on HLA B27.1 at the MHC groove region (within sequence 65-85), and to a Klebsiella pneumoniae nitrogenase reductase peptide (within sequence 181-199)

    OpenAIRE

    1990-01-01

    74 overlapping peptides of varying lengths from Klebsiella pneumoniae nitrogenase reductase (residues 181-199) and from the HLA B27.1 molecule (residues 65-85) were synthesized and tested by ELISA against sera from HLA B27+ ankylosing spondylitis (AS) patients, and sera from HLA B27+ and HLA B27- healthy first-degree relatives. Antibody activity in AS sera to Klebsiella peptides of four to eight amino acids was maximal with the peptide NSRQTDR. Activity to HLA B27 peptides was maximal with th...

  18. Process for producing astatine-211 for radiopharmaceutical use

    International Nuclear Information System (INIS)

    A one-step chemical manipulation is described in combination with a distillation and collection process for producing At-211 comprising; a. providing a target of irradiated Bismuth coated to a predetermined thickness of a backing member, b. providing a vapor-producing still operably connected with a condenser that has a water cooled condensate collector formed of a dry silica gel mesh maintained at a temperature above the freezing point of water, and providing an effluent gas filter that is operably connected to receive effluent gas from the condenser, c. heating the target in the still at a temperature in the range of about 6300-6800C for a time period in the range of 50 to 80 minutes, to evole At-211 vapor from the target, c. providing a dry carrier gas having an oxygen concentration that is sufficient to form Bi/sub 2/O/sub 3/ thereby to essentially preclude vaporization of Bi metal, passing the carrier gas through the still to carry the At-211 vapor to the condenser, and to carry effluent from the condenser to the effluent gas filter, e. eluting At-211 from the condensate collector of the condenser with a controlled volume of eluent containing predetermined solvents that are compatible with a given desired radiopharmaceutical procedure, and f. collecting the At-211 in the controlled volume of eluent for use in the given radiopharmaceutical procedure

  19. ZZ VITJEFF31.BOLIB,JEFF-3.1 Multigroup Coupled (199 n + 42 gamma) X-Section Library in AMPX Format for Nuclear Fission Applications

    International Nuclear Information System (INIS)

    1 - Description: VITJEFF31.BOLIB /1/ /2/ /3/ is a multigroup coupled (199 neutron groups + 42 photon groups) pseudo-problem-independent cross section library in AMPX /4/ format for nuclear fission applications. VITJEFF31.BOLIB is based on the JEFF-3.1 /3/ /5/ European nuclear data file processed, through the NJOY-99.160 /6/ system with an ENEA-Bologna revised GROUPR module /7/ and the ENEA-Bologna 2007 Revision /8/ of the SCAMPI /9/ system, in the VITAMIN-B6 /10/ energy group structure using the same parameters and calculation procedures. Original nuclear data file: JEFF-3.1. Data processing systems: ENEA-Bologna Revised Versions of NJOY-99.160 and SCAMPI. Format: AMPX. Number of groups: 199 neutron groups and 42 photon groups; Thermal neutron groups: 36 groups below 5.043 eV with up-scattering cross sections; Neutron energy range: 1.0 E-5 eV - 19.64 MeV; Photon energy range: 1.0 keV - 30.0 MeV; Temperatures [K]: 300, 600, 1000, and 2100 (same values as in VITAMIN-B6); Background cross sections (Sigma-zeros) [barns]: 1, 10, 50, 100, 300, 1.0 E+3, 1.0 E+4, 1.0 E+5, 1.0 E+6, 1.0 E+10 (infinite dilution) (same values as in VITAMIN-B6); Legendre order: P7 for materials with Z ≤ 29 (copper); P5 for the remainders; Number of materials: 182. Neutron Weighting spectrum: Corresponding to the IWT=4 input option in the GROUPR module of NJOY: from 1.0 E-5 eV to 0.125 eV → Maxwellian thermal spectrum (kT = 0.025 eV); from 0.125 eV to 820.8 keV → '1/E' slowing-down spectrum; from 820.8 keV to 19.64 MeV → fission spectrum (fission temperature = 1.273 MeV). Photon Weighting spectrum: Corresponding to the IWT=3 input option in the GAMINR module of NJOY: '1/E' spectrum with a 'roll-off' at lower energies to represent photoelectric absorption and a similar 'drop-off' at higher energies corresponding to the Q-value for neutron capture. The total (prompt + delayed) neutron fission spectra for U-235, U-238 and Pu-239 are included in tabulated form in the package together with

  20. ZZ VITJEF22.BOLIB, JEF-2.2 Multigroup Coupled (199 n + 42 gamma) X-Section Library in AMPX Format for Nuclear Fission Applications

    International Nuclear Information System (INIS)

    1 - Description or function: VITJEF22.BOLIB /1/ is a multigroup coupled (199 neutron groups + 42 photon groups) pseudo-problem-independent cross section library in AMPX /2/ format for nuclear fission applications. VITJEF22.BOLIB is based on the JEF-2.2 /3/ European nuclear data file and it was processed, through the NJOY /4/ and SCAMPI /5/ systems, in the VITAMIN-B6 /6/ group structure using the same parameters and calculation procedures. Original nuclear data file: JEF-2.2. Data processing systems: NJOY-94.66 and SCAMPI. Format: AMPX. Number of groups: 199 neutron groups and 42 photon groups. Thermal neutron groups: 36 groups below 5.043 eV with up-scattering cross sections. Neutron energy range: 1.0 E-5 eV - 19.64 MeV. Photon energy range: 1.0 keV - 30.0 MeV. Temperatures [K]: 300, 600, 1000, and 2100 (same values as in VITAMIN-B6). Background cross sections (SIGMA-Zeros) [barns]: 1, 10, 50, 100, 300, 1.0 E+3, 1.0 E+4, 1.0 E+5, 1.0 E+6, 1.0 E+10 (infinite dilution) (same values as in VITAMIN-B6). Legendre order: P7 for materials with Z ≤ 29 (copper); P5 for the remainders. Number of materials: 138. Materials included: one file per material (nat=natural): H-H2O, H-CH2, H2-D2O, H-3, He-3, He-4, Li-6, Li-7, Be-9, Be-TH, B-10, B-11, C-nat, C-GPH, N-14, N-15, O-16, O-17, F-19, Na-23, Mg-nat, Al-27, Si-nat, P-31, S-32, S-33, S-34, S-36, Cl-nat, K-nat, Ca-nat, Ti-nat, V-nat, Cr-50, Cr-52, Cr-53, Cr-54, Mn-55, Fe-54, Fe-56, Fe-57, Fe-58, Co-59, Ni-58, Ni-60, Ni-61, Ni-62, Ni-64, Cu-nat, Ga-nat, Y-89, Zr-nat, Nb-93, Mo-nat, Ag-107, Ag-109, Cd-nat, Cd-106, Cd-110, Cd-111, Cd-112, Cd-113, Cd-114, Cd-115m, Cd-116, In-113, In-115, Sn-112, Sn-114, Sn-115, Sn-116, Sn-117, Sn-118, Sn-119, Sn-120, Sn-122, Sn-123, Sn-124, Sn-125, Sn-126, Ba-138, Eu-151, Eu-152, Eu-153, Eu-154, Eu-155, Hf-174, Hf-176, Hf-177, Hf-178, Hf-179, Hf-180, Ta-181, Ta-182, W-182, W-183, W-184, W-186, Re-185, Re-187, Au-197, Pb-nat, Bi-209, Th-230, Th-232, Pa-231, Pa-233, U-232, U-233, U-234, U-235, U-236

  1. Arctigenin Confers Neuroprotection Against Mechanical Trauma Injury in Human Neuroblastoma SH-SY5Y Cells by Regulating miRNA-16 and miRNA-199a Expression to Alleviate Inflammation.

    Science.gov (United States)

    Song, Jie; Li, Na; Xia, Yang; Gao, Zhong; Zou, Sa-Feng; Yan, Yu-Hui; Li, Shao-Heng; Wang, Yue; Meng, Ya-Kun; Yang, Jing-Xian; Kang, Ting-Guo

    2016-09-01

    Mechanical trauma injury is a severe insult to neural cells. Subsequent secondary injury involves the release of inflammatory factors that have dramatic consequences for undamaged cells, leading to normal cell death after the initial injury. The present study investigated the capacity for arctigenin (ARC) to prevent secondary effects and evaluated the mechanism underlying the action of microRNA (miRNA)-199a and miRNA-16 in a mechanical trauma injury (MTI) model using SH-SY5Y cells in vitro. SH-SY5Y cells are often applied to in vitro models of neuronal function and differentiation. Recently, miRNAs have been demonstrated to play a crucial role in NF-κB and cholinergic signaling, which can regulate inflammation. The cell model was established by scratch-induced injury of human SH-SY5Y cells, which mimics the characteristics of MTI. A cell counting kit-8 (CCK-8), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and immunocytochemistry were used to measure cell viability. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the inflammatory cytokine and cholinesterase (CHE) content. The lactate dehydrogenase (LDH) content was measured to assess the degree of cell injury. The mRNA levels were measured by RT-PCR to analyze ARC's mechanism of action. miRNA inhibitors and mimics were used to inhibit and strengthen the expression of miRNAs. Protein expression was detected by western blotting analysis. ARC treatment reduced the TNF-α and IL-6 levels as well as the number of TUNEL+ apoptotic SH-SY5Y cells surrounding the scratch and increased the IL-10 level compared to the controls. ARC attenuated the increase of the cell damage degree and LDH content induced by scratching, indicating increased cell survival. Mechanistic studies showed that ARC upregulated the miRNA-16 and miRNA-199a levels to reduce upstream protein (IKKα and IKKβ) expression and inhibit NF-κB signaling pathway activity; moreover, the increased miRNA-199a suppresses

  2. Structure and electrochemical properties of La, F dual-doped LiLa0.01Mn1.99O3.99F0.01 cathode materials

    Institute of Scientific and Technical Information of China (English)

    Meng Chen; Shengjun Li; Chuang Yang

    2008-01-01

    The cathode materials LiMn204 and rare earth elements La-doped or La and F dual-doped spinel lithium manganese oxides were synthesized by the citric acid-assisted sol-gel method. The synthesized samples were investigated by differential thermal analysis (DTA) and thermogravimetry (TG) measurements, X-ray diffraction (XRD), scanning electronic microscope (SEM), cyclic voltammetry (CV), and charge-discharge test. XRD data shows that all the samples exhibit the same pure spinel phase, and the LiLa0.01Mn1.9903.99F0.01 and LiLao.01Mnl.9904 samples have smaller lattice parameters and unit cell volume than LiMn2O4. SEM indicates that LiLao.01Mnl.9903.99F0.01 has a slightly smaller particle size and a more regular morphology structure with narrow size distribution. The charge-discharge test reveals that the initial capacities of LiMn2O4, LiLa0mMnl.9904, and LiLa0.01Mn1.99O3.99F0.01 are 129.9, 122.8, and 126.4 mAh-g-1, and the capacity losses of the initial values after 50 cycles are 14.5%, 7.6%, and 8.0%, respectively.The CVs show that the La and F dual-doped spinel displays a better reversibility than LiMn2O4.

  3. 49 CFR 199.200 - Purpose.

    Science.gov (United States)

    2010-10-01

    ... prevent accidents and injuries resulting from the misuse of alcohol by employees who perform covered... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY DRUG AND ALCOHOL TESTING Alcohol...

  4. 32 CFR 199.8 - Double coverage.

    Science.gov (United States)

    2010-07-01

    ... ECHO benefit was requested. Benefits eligible for payment under a state plan for medical assistance... (for example, the Indian Health Service); or (v) State Victims of Crime Compensation Programs. (c...-sharing amounts (and deductible if using a retail non-network pharmacy). Part D plan sponsors may offer...

  5. QTL Information Table: 199 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available Morphological trait Seed 1000-grain weight RFLP C)Interval RIL Lemont Teqing A CDO118 CDO455 pha ... c Loci Are the Primary Genetic Basis of Inbreeding Depression ... and Heterosis in Rice. II. Grain Yield Components. ...

  6. 46 CFR 199.30 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... Coast Guard activities within a Coast Guard District. Coast Guard Districts are described in 33 CFR part.... Marine Inspection Zones are described in 33 CFR part 2. Passenger means— (a) On an international voyage... limnology, or both, or only in oceanographic or limnological research, including those studies about the...

  7. 32 CFR 199.3 - Eligibility.

    Science.gov (United States)

    2010-07-01

    ... victim of the abuse. (2) Abused child. The abused child must: (i) Under paragraph (b)(2)(iii)(A)(1) of... Option. (7) Beginning dates of eligibility for children who are victims of abuse, including incapacitated... former spouses, some dependents who are victims of abuse and some incapacitated children as......

  8. 32 CFR 199.11 - Overpayments recovery.

    Science.gov (United States)

    2010-07-01

    ... part are also issued under Treasury regulations implementing the DCIA (31 CFR part 285) and related statutes and regulations governing the offset of Federal salaries (5 U.S.C. 5514; 5 CFR part 550, subpart K... regulations issued by the Department of the Treasury (Treasury) and the Department of Justice (DOJ) (31...

  9. 30 CFR 19.9 - Performance.

    Science.gov (United States)

    2010-07-01

    ... period a mean candlepower of light beam of not less than 1. (b) Bulb life. The average life of the bulbs.... The life of a bulb is the number of hours its main filament will burn in the cap lamp or its equivalent. The life of a bulb having main filaments in parallel is considered ended when the first...

  10. Re-evaluation of microscopic and integral cross-section data for important dosimetry reactions. Re-evaluation of the excitation functions for the 24Mg(n,p)24Na, 32S(n,p)32P, 60Ni(n,p)60m+gCo, 63Cu(n,2n)62Cu, 65Cu(n,2n)64Cu, 64Zn(n,p)64Cu, 115In(n,2n)114mIn, 127I(n,2n)126I, 197Au(n,2n)196Au and 199Hg(n,n')199mHg reactions

    International Nuclear Information System (INIS)

    Re-evaluations of cross sections and their associated covariance matrices have been carried out for ten dosimetry reactions: - excitation functions for the 63Cu(n,2n)62Cu, 65Cu(n,2n)64Cu, 64Zn(n,p)64Cu, 115In(n,2n)114mIn and 199Hg(n,n')199mHg reactions were re-evaluated over the neutron energy range from threshold to 20 MeV; - excitation functions for the 24Mg(n,p)24Na, 32S(n,p)32P and 60Ni(n,p)60m+gCo were reevaluated in the energy range from threshold to 21 MeV; - excitation functions for the 127I(n,2n)126I and 197Au(n,2n)196Au reactions were reevaluated in the energy range from threshold to 32 and 40 MeV, respectively. Benchmark calculations performed for 235U thermal fission and 252Cf spontaneous fission neutron spectra show that the integral cross sections derived from the newly evaluated excitation functions exhibit improved agreement with related experimental data when compared with the equivalent data from the IRDF-2002 library. (author)

  11. Duodenal Duplication Cyst with Profound Elevation of Intracystic Carbohydrate Antigen (CA 19-9 and Carcinoembryonic Antigen (CEA: A Rare but Important Differential in the Diagnosis of Cystic Tumours of the Pancreas

    Directory of Open Access Journals (Sweden)

    Kant J Shah

    2006-03-01

    Full Text Available The diagnosis of cystic tumours of the pancreas can be difficult. The principal effort is directed towards distinguishing benign post-inflammatory pseudocysts from true cystic tumours [1, 2]. Pointers that may help to identify a cystic tumour include the presence of a thick or irregular lining with septae on cross-sectional imaging [2]. Further, endoscopic ultrasound (EUS with linear EUS-guided aspiration can aid this process of differentiation as cyst fluid can be analysed for tumour markers, amylase and cytology [3, 4]. Elevated levels of intra-cyst tumour markers favour the diagnosis of a true cystic lesion [5]. It is important to make the distinction between post-inflammatory pseudocysts and cystic tumours as the natural history of these conditions is different and this is reflected in their different optimal management. Cystic lesions carry a varying risk of malignant transformation and most require resection [2]. Further, mistaking a cystic tumour for a pseudocyst can have disastrous consequences and lead to inappropriate drainage surgery [6]. In practice, cystic tumours and pseudocysts are the principal differential diagnoses to be assessed in a patient with a pancreatic cystic lesion; however a third rare possible differential is that of duplication cyst. These are rare congenital abnormalities of the gut characterized by the presence of smooth muscle in the wall and can be lined with gastrointestinal mucosa [7]. Little is known of the natural history and malignant potential of these lesions and as a result of their rarity, knowledge of their presentation accrues principally through single case studies. This paper reports a patient presenting with a pancreatic cystic lesion who underwent resection on the grounds of having a complex cyst with a profoundly elevated intra-cystic carbohydrate antigen (CA 19-9 level together with carcinoembryonic antigen (CEA. Final histology revealed a duodenal duplication cyst without malignancy. To the

  12. Yeast Interacting Proteins Database: YJL199C, YJL199C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available d in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies...cies; protein detected in large-scale protein-protein interaction studies Rows with this prey as prey (4) Ro...n; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies... species; protein detected in large-scale protein-protein interaction studies Rows with this prey as prey Ro

  13. Value of Serum GGT,ALP,ADA and CA19 - 9 in the Diagnosis of Jaundice%血清谷氨酰转肽酶、碱性磷酸酶、腺苷脱氨酶联合糖类抗原 CA19-9在黄疸鉴别中的意义

    Institute of Scientific and Technical Information of China (English)

    倪丽娜; 栾希英

    2014-01-01

    Objective To investigate the value of serum GGT,ALP,ADA and CA19 - 9 in the diagnosis of jaun-dice. Methods 614 cases with jaundice were admitted to our hospital from January 2011 to October 2013,were divided into hepatocellular and obstructive jaundice group according to the type,and the patients in obstructive jaundice group were divided into benign obstructive jaundice group and malignant obstructive jaundice group. The distribution of serum GGT,ALP,ADA and CA19 - 9 in each group were analyzed. Results Compared with hepatocellular jaundice group,the serum level of GGT and ALP were higher in obstructive jaundice group,the differences were statistically significant(P < 0. 05);Compared with obstruc-tive jaundice group,the serum level of ADA was higher in hepatocellular Jaundice group,the difference was statistically signifi-cant(P < 0. 05);Compared with benign obstructive jaundice group,the serum levels of GGT,ALP and CA19 - 9 were higher in malignant obstructive jaundice group,the differences were statistically significant(P < 0. 05). Conclusion Jaundice with marked elevation of serum GGT and ALP while no significant elevation of serum ADA is considered as obstructive jaundice;Jaundice with marked elevation of serum ADA while mild elevation of serum GGT and ALP are considered as hepatocellular jaun-dice;Jaundice with marked elevation of serum GGT,ALP and CA19 - 9 are considered as malignant obstructive jaundice.%目的:探讨血清谷氨酰转肽酶( GGT)、碱性磷酸酶( ALP)、腺苷脱氨酶( ADA)、糖类抗原CA19-9升高程度在黄疸类型鉴别中的价值。方法选取本院2011年1月-2013年10月收治的黄疸患者614例,根据黄疸类型将614位患者分为肝细胞性黄疸组和梗阻性黄疸组,后者又分为良性梗阻性黄疸和恶性梗阻性黄疸两组,分析 GGT、ALP、ADA、CA19-9水平在各组间分布差异。结果 GGT、ALP 值在梗阻性黄疸组中较肝细胞性黄疸组高,

  14. Evaluation of a Wet Chemistry Method for Isolation of Cyclotron Produced [211At]Astatine

    Directory of Open Access Journals (Sweden)

    Shigeki Watanabe

    2013-09-01

    Full Text Available A “wet chemistry” approach for isolation of 211At from an irradiated bismuth target is described. The approach involves five steps: (1 dissolution of bismuth target in conc. HNO3; (2 removal of the HNO3 by distillation; (3 dissolution of residue in 8 M HCl; (4 extraction of 211At from 8 M HCl into DIPE; and (5 extraction of 211At from DIPE into NaOH. Results from 55 “optimized” 211At isolation runs gave recovery yields of approximately 78% after decay and attenuation corrections. An attenuation-corrected average of 26 ± 3 mCi in the target provided isolated (actual yields of 16 ± 3 mCi of 211At. A sixth step, used for purification of 211At from trace metals, was evaluated in seven runs. In those runs, isolated 211At was distilled under reductive conditions to provide an average 71 ± 8% recovery. RadioHPLC analyses of the isolated 211At solutions, both initial and after distillation, were obtained to examine the 211At species present. The primary species of 211At present was astatide, but astatate and unidentified species were also observed. Studies to determine the effect of bismuth attenuation on 211At were conducted to estimate an attenuation factor (~1.33 for adjustment of 211At readings in the bismuth target.

  15. Final Report for grant entitled "Production of Astatine-211 for U.S. Investigators"

    Energy Technology Data Exchange (ETDEWEB)

    Wilbur, Daniel Scott

    2012-12-12

    Alpha-particle emitting radionuclides hold great promise in the therapy of cancer, but few alpha-emitters are available to investigators to evaluate. Of the alpha-emitters that have properties amenable for use in humans, 211At is of particular interest as it does not have alpha-emitting daughter radionuclides. Thus, there is a high interest in having a source of 211At for sale to investigators in the US. Production of 211At is accomplished on a cyclotron using an alpha-particle beam irradiation of bismuth metal. Unfortunately, there are few cyclotrons available that can produce an alpha particle beam for that production. The University of Washington has a cyclotron, one of three in the U.S., that is currently producing 211At. In the proposed studies, the things necessary for production and shipment of 211At to other investigators will be put into place at UW. Of major importance is the efficient production and isolation of 211At in a form that can be readily used by other investigators. In the studies, production of 211At on the UW cyclotron will be optimized by determining the best beam energy and the highest beam current to maximize 211At production. As it would be very difficult for most investigators to isolate the 211At from the irradiated target, the 211At-isolation process will be optimized and automated to more safely and efficiently obtain the 211At for shipment. Additional tasks to make the 211At available for distribution include obtaining appropriate shipping vials and containers, putting into place the requisite standard operating procedures for Radiation Safety compliance at the levels of 211At activity to be produced / shipped, and working with the Department of Energy, Isotope Development and Production for Research and Applications Program, to take orders, make shipments and be reimbursed for costs of production and shipment.

  16. Astatination of nanoparticles containing silver as possible carriers of 211At

    Czech Academy of Sciences Publication Activity Database

    Lebeda, Ondřej; Kučka, Jan; Hrubý, Martin; Koňák, Čestmír; Kozempel, Ján

    2006-01-01

    Roč. 64, - (2006), s. 201-206. ISSN 0969-8043 R&D Projects: GA AV ČR KJB4048302; GA AV ČR KJB4050408 Institutional research plan: CEZ:AV0Z1048901 Keywords : nanoparticles * 211AT * Atalpha particle therapy Subject RIV: CH - Nuclear ; Quantum Chemistry Impact factor: 0.924, year: 2006

  17. Production of Astatine-211 at the Duke University Medical Center for its regional distribution

    Energy Technology Data Exchange (ETDEWEB)

    Zalutsky, Michael [Duke University Medical Center, Durham, NC (United States)

    2016-01-01

    Systemic targeted radiation therapy and radioimmunotherapy continue to be important tools in the treatment of certain cancers. Because of their high energy and short path length, alpha particle emitters such as 211At are more effective than either external beam x- ray or in vivo beta radiation in delivering potentially curative doses of radiation. The limited clinical trials that have been conducted to date have yielded encouraging responses in some patients, e.g., malignant brain tumors. In order to escalate the additional necessary research and development in radiochemistry, radiobiology and efficacy evaluation of alpha particle radiotherapeutics, it is universally agreed that access to an affordable, reliable supply of 211At is warranted. In conjunction with the Department of Energy's intent to enhance stable and radioactive isotope availability for research applications, it is the primary objective of this project to improve 211At production and purification capabilities at Duke so that this radionuclide can be supplied to researchers at other institutions throughout the US.The most widely used 211At production method involves the α,2n reaction on Bismuth using a cyclotron with beams ≤ 28 MeV. Yields can be enhanced with use of an internal target that allows for a higher alpha fluence plus efficient heat dissipation in the target. Both of these items are in place at Duke; however, in order to support production for multi-institutional use, irradiation campaigns in excess of 50 µAp and four hours duration will be needed. Further, post-irradiation processing equipment is lacking that will enable the distribution process. Financial support is sought for i) a shielded, ventilated processing/containment hood; ii) development of a post-irradiation target retrieval system; iii) fabrication of a 211At distillation and recovery module and iv) a performance review and, where needed, an enhancement of seven major subsystems that comprise the CS-30 Cyclotron. With these modifications in place, routine production of ≥200 mCi of At-211 should be readily achievable, given our methodological development of At-211 target preparation, internal target irradiation and dry distillation to recover the radionuclide.

  18. 32 CFR 199.23 - Special Supplemental Food Program.

    Science.gov (United States)

    2010-07-01

    ...-videoconferencing, real-time computer-based distance learning, or other means. (iv) Nutrition education services... prescribed in USDA regulations at 7 CFR Part 246.11. (i) The WIC Overseas nutrition education program shall... supplemental foods and nutrition education, at no cost, to eligible persons and to serve as an adjunct to...

  19. 32 CFR 199.6 - TRICARE-authorized providers.

    Science.gov (United States)

    2010-07-01

    ... the 50 states, the District of Columbia, Puerto Rico, Virgin Islands, American Samoa, Guam, Wake..., appointed by the governing body, shall be a psychiatrist or doctoral level psychologist who meets applicable... activities is vested in a psychiatrist or doctoral level psychologist. The management of medical care...

  20. The Management of the Standards: Standards for Accreditation, 199X.

    Science.gov (United States)

    Robbins, Jane B.

    1992-01-01

    This report on the development of the American Library Association's revised standards for master's level programs in library and information studies describes the principal activities preceding the current revision process and details key changes to and retentions from the 1972 Standards. A timetable of remaining steps is included. (NRP)

  1. 49 CFR 173.199 - Category B infectious substances.

    Science.gov (United States)

    2010-10-01

    ... in paragraph (a)(9) of this section, a Category B infectious substance meeting the definition of a... maximum temperatures, changes in humidity and pressure, and shocks, loadings and vibrations...

  2. 27 CFR 26.199c - Action by carrier.

    Science.gov (United States)

    2010-04-01

    ..., DEPARTMENT OF THE TREASURY LIQUORS LIQUORS AND ARTICLES FROM PUERTO RICO AND THE VIRGIN ISLANDS Shipment of Bulk Distilled Spirits From Puerto Rico, Without Payment of Tax, for Transfer From Customs Custody to... 5110.31 shall, at the time of unlading at the port of arrival in the United States, segregate...

  3. 26 CFR 1.199-0 - Table of contents.

    Science.gov (United States)

    2010-04-01

    ...) Qualified computer software maintenance agreements. (F) Advertising income and product-placement income. (v...) Deductions for charitable contributions. (3) Research and experimental expenditures. (4) Deductions...

  4. Gammastrahlung vom Einfang thermischer Neutronen in Hg 199

    DEFF Research Database (Denmark)

    Maier, B.P.; Gruber, U.; Koch, H. R.;

    1965-01-01

    103 gamma transitions in Hg 200 have been observed. 37 of them were fitted into a level scheme which comprises 17 states. The energy of the one-phonon vibrational stateI=2+ has been determined to be 367.970±0.020 keV, that of the two-phonon vibrational triplet 947.34±0.07 keV, 1029.37±0.05 keV an...

  5. 46 CFR 199.180 - Training and drills.

    Science.gov (United States)

    2010-10-01

    ... must be incorporated into the onboard training sessions described in paragraph (g) of this section. (2... be remedied as soon as possible. (g) Onboard training and instruction. (1) Onboard training in the...) Onboard training in the use of davit-launched liferafts must take place at intervals of not more than...

  6. 32 CFR 199.21 - Pharmacy benefits program.

    Science.gov (United States)

    2010-07-01

    ... difference between the cost shares of a formulary versus non-formulary drug. The amount for each of multiple... the Federal Employees Health Benefits Program under 5 U.S.C. 8909(f). (p) General fraud, abuse, and conflict of interest requirements under TRICARE pharmacy benefits program. All fraud, abuse, and...

  7. 32 CFR 199.10 - Appeal and hearing procedures.

    Science.gov (United States)

    2010-07-01

    ... a determination of abuse or fraudulent practices or procedures under another Federal or federally... dispute is calculated as the amount of money CHAMPUS would pay if the services and supplies involved in dispute were determined to be authorized CHAMPUS benefits. Examples of amounts of money that are...

  8. 32 CFR 199.14 - Provider reimbursement methods.

    Science.gov (United States)

    2010-07-01

    ... standardize the charges for the cost effects of indirect medical education factors, each teaching hospital's... (including but not limited to direct education and capital costs and indirect education factors) for which... rural hospitals and an adjustment for area wage differences and indirect medical education...

  9. 26 CFR 1.199-3 - Domestic production gross receipts.

    Science.gov (United States)

    2010-04-01

    ... order. With respect to a particular order by a customer of 100 television sets, some were manufactured... gross receipts derived from the MPGE of QPP to qualify as DPGR. (2) Packaging, repackaging, labeling, or... taxpayer engages in no other MPGE activity with respect to that QPP, the taxpayer's packaging,...

  10. Comprehensive multiplatform biomarker analysis of 199 anal squamous cell carcinomas.

    Science.gov (United States)

    Smaglo, Brandon G; Tesfaye, Anteneh; Halfdanarson, Thorvardur R; Meyer, Joshua E; Wang, Jue; Gatalica, Zoran; Reddy, Sandeep; Arguello, David; Boland, Patrick M

    2015-12-22

    Anal squamous cell carcinoma (ASCC) is a rare, HPV-associated malignancy typically diagnosed in early stages and definitively treated with chemoradiation. In situations where patients exhibit metastatic or recurrent disease, treatment options are severely limited. In this study, molecular alterations were identified that could be used to aid in therapeutic decisions for patients with metastatic or recurrent anal squamous cell carcinoma. Specimens from patients with this cancer were tested via a multiplatform profiling service (Caris Life Sciences, Phoenix, AZ) consisting of gene sequencing, protein expression by immunohistochemistry, and gene amplification with in situ hybridization. Utilizing these techniques, novel treatment strategies that could be explored were identified, including potential benefit with anti-EGFR therapies, immune checkpoint inhibitors, topoisomerase inhibitors, and taxanes. The frequency of overexpression of proteins that mark resistance to chemotherapeutic drugs, such as MRP1 (chemotherapy efflux pump), ERCC1 (resistance to platinum-based chemotherapy), and thymidylate synthase (resistance to fluoropyrimidines) were also identified, suggesting a lack of benefit. This multiplatform strategy could be explored for its potential to generate a personalized treatment selection for patients with advanced ASCC, provide a guide for future therapeutic development for this cancer, and be extended to other rare cancer types as well. PMID:26498363

  11. 32 CFR 199.22 - TRICARE Retiree Dental Program (TRDP).

    Science.gov (United States)

    2010-07-01

    ... Affairs pursuant to Section 1712 of Title 38, United States Code, and 38 CFR 17.93, 17.161, or 17.166..., preventive services, basic restorative services (including endodontics), oral surgery services, and emergency...) Pulp capping. (B) Pulpotomy and pulpectomy. (C) Root canal therapy. (D) Apexification...

  12. 49 CFR 199.113 - Employee assistance program.

    Science.gov (United States)

    2010-10-01

    ... following elements: display and distribution of informational material; display and distribution of a community service hot-line telephone number for employee assistance; and display and distribution of the... one 60-minute period of training on the specific, contemporaneous physical, behavioral,...

  13. Sucrose-aided combustion synthesis of nanosized LiMn{sub 1.99-y}Li{sub y}M{sub 0.01}O{sub 4} (M = Al{sup 3+}, Ni{sup 2+}, Cr{sup 3+}, Co{sup 3+}, y = 0.01 and 0.06) spinels. Characterization and electrochemical behavior at 25 and at 55 C in rechargeable lithium cells

    Energy Technology Data Exchange (ETDEWEB)

    Amarilla, J.M.; Pico, F.; Rojo, J.M.; Rojas, R.M. [Instituto de Ciencia de Materiales de Madrid, Consejo Superior de Investigaciones Cientificas (CSIC), Sor Juana Ines de la Cruz no. 3, Cantoblanco, 28049 Madrid (Spain); Petrov, K.; Avdeev, G. [Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria)

    2009-06-15

    Doubly doped LiMn{sub 1.99-y}Li{sub y}M{sub 0.01}O{sub 4} (M = Al{sup 3+}, Ni{sup 2+}, Cr{sup 3+}, Co{sup 3+}; y = 0.01 and 0.06) spinels have been synthesized by the sucrose-aided combustion method. Combined TG/DTA and XRD studies have shown that stoichiometric single-phase spinels are formed after annealing of the samples at 700 C for 1 h. The samples obtained are nanocrystalline materials having a narrow size-distribution and a coherent domain size between 40 and 60 nm, depending on the amount of fuel (sucrose) used in the synthesis. The influence of the Li-excess, the type of M{sup n+}-dopant cation and the amount of fuel used in the synthesis on the electrochemical behavior of the spinels in a Li-cell at room and at elevated temperature (55 C) has been studied. At 25 C all the spinels synthesized have a good capacity retention after 100 cycles, QRt-100 > 92%. At 55 C the increase of the Li-excess improves the cycling performances. Rate capability studies show that the spinels retain >90% of their capacity even at 5C rate. The synergic effect of the Li-excess and the particle size on the electrochemical properties of the spinels as cathode material has been settled. The LiMn{sub 1.93}Li{sub 0.06}M{sub 0.01}O{sub 4}, (M = Al{sup 3+}, Ni{sup 2+}) spinels, with cyclabilities >99.9% by cycle at both 25 and 55 C, and high rate capabilities, are the ones that show the best electrochemical properties. (author)

  14. 26 CFR 1.199-9 - Application of section 199 to pass-thru entities for taxable years beginning on or before May 17...

    Science.gov (United States)

    2010-04-01

    ... the Trust's direct expenses, $1,000 of the trustee commissions, the $1,000 of real estate taxes, and... activities that generate DPGR are within Standard Industrial Classification (SIC) Industry Group AAA (SIC AAA). All of PRS's production activities that generate non-DPGR are within SIC Industry Group BBB (SIC...

  15. Astatine-211 conjugated to an anti-CD20 monoclonal antibody eradicates disseminated B-cell lymphoma in a mouse model

    Energy Technology Data Exchange (ETDEWEB)

    Green, Damian J.; Shadman, Mazyar; Jones, Jon C.; Frayo, Shani; Kenoyer, Aimee L.; Hylarides, Mark; Hamlin, Donald K.; Wilbur, D. Scott; Balkan, Ethan R.; Lin, Yukang; Miller, Brian W.; Frost, Sophia; Gopal, Ajay K.; Orozco, Johnnie J.; Gooley, Ted; Laird, Kelley L.; Till, B. G.; Back, Tom; Sandmaier, B. M.; Pagel, John M.; Press, Oliver W.

    2015-03-26

    Alpha emitting radionuclides release a large amount of energy within a few cell diameters and may be particularly effective for radioimmunotherapy targeting minimal residual disease (MRD) conditions in which micrometastatic disease satellites are broadly distributed. To evaluate this hypothesis, 211At conjugated 1F5 mAb (anti-CD20) was studied in both bulky lymphoma tumor xenograft and MRD animal models. Superior treatment responses to 211At conjugated 1F5 mAb were evident in the MRD setting. Lymphoma xenograft tumor bearing animals treated with doses of up to 48µCi of anti-CD20 211At-decaborate [211At-B10-1F5] experienced modest responses (0% cures but 2-3-fold prolongation of survival compared to negative controls). In contrast, 70% of animals in the MRD lymphoma model demonstrated complete eradication of disease when treated with 211At-B10-1F5 at a radiation dose that was less than one-third (15 µCi) of the highest dose given to xenograft animals. Tumor progression among untreated control animals in both models was uniformly lethal. After 130 days, no significant renal or hepatic toxicity is observed in the cured animals receiving 15 µCi of 211At-B10-1F5. These findings suggest that in a MRD lymphoma model, where isolated cells and tumor microclusters prevail, α-emitters may be uniquely efficacious.

  16. An all-solid state laser system for the laser ion source RILIS and in-source laser spectroscopy of astatine at ISOLDE,CERN

    OpenAIRE

    Rothe, Sebastian

    2012-01-01

    This doctoral thesis describes the extension of the resonance ionization laser ion source RILIS at CERN/ISOLDE by the addition of an all-solid state tunable titanium:sapphire (Ti:Sa) laser system to complement the well-established system of dye lasers. Synchronous operation of the so called Dual RILIS system of Ti:Sa and dye lasers was investigated and the potential for increased ion beam intensity, reliability, and reduced setup time has been demonstrated. In-source resonance ionization spec...

  17. Inactivation of human osteosarcoma cells in vitro by {sup 211}At-TP-3 monoclonal antibody: Comparison with astatine-211 and external-beam X rays

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, R.H. [Univ. of Oslo (Norway)]|[Institute for Cancer Research, Oslo (Norway); Bruland, O.S. [Institute for Cancer Research, Oslo (Norway); Hoff, P.; Alstad, J. [Univ. of Oslo (Norway); Lindmo, T. [Institute for Cancer Research, Oslo (Norway); Rofstad, E.K. [Norwegian Institute of Technology, Trondheim (Norway)

    1994-08-01

    The potential usefulness of {alpha}-particle radioimmunotherapy in the treatment of osteosarcoma was studied in vitro by using the monoclonal antibody TP-3 and cells of three human osteosarcoma cell lines (OHS, SAOS and KPDX) differing in antigen expression. Cell survival curves were established after treatment with (a) {sup 211}At-TP-3 of different specific activities, (b) {sup 211}At-labeled bovine serum albumin (BSA), (c) free {sup 211}At and (d) external-beam X rays. The three osteosarcoma cell lines showed similar survival curves, whether treated with external-beam X rays, {sup 211}At-BSA or free {sup 211}At. The D{sub o}`s were lower for free {sup 211}At than for {sup 211}At-BSA. The survival curves for {sup 211}At-TP-3 treatment, on the other hand, differed significantly among the cell lines, suggesting that sensitivity to {sup 211}At-TP-3 treatment was governed by cellular properties other than sensitivity to external-beam X rays. The cellular property most important for sensitivity to {sup 211}At-TP-3 treatment was the antigen expression. Cell inactivation after {sup 211}At-TP-3 treatment increased substantially with increasing specific activity of the {sup 211}At-TP-3. At high specific activities, the cytotoxic effect of {sup 211}At-TP-3 was significantly higher than that of {sup 211}At-BSA. In conclusion, {sup 211}At-TP-3 has the potential to give clinically favorable therapeutic ratios in the treatment of osteosarcoma. 39 refs., 5 figs., 2 tabs.

  18. [Bifunctional chelates of Rh-105, Au-199, and other metallic radionuclides as potential radiotherapeutic agents

    International Nuclear Information System (INIS)

    Progress during this period is reported under the following headings: Diethylenetriamine based and related bifunctional chelating agents and their complexation with Rh-105, Au-198, Pd-109, cu-67, In-111, and Co-57; studies of Pd-109, Rh-105 and Tc-99m with bifunctional chelates based on phenylenediamine; establishment of an appropriate protein assay method for conjugated proteins; studies of new bifunctional Bi, Tri and tetradentate amine oxime ligands with Rh-105; IgG and antibody B72.3 conjugation studies by HPLC Techniques with bifunctional metal chelates; and progress on ligand systems for Au(III)

  19. 26 CFR 1.199-4 - Costs allocable to domestic production gross receipts.

    Science.gov (United States)

    2010-04-01

    ... for a taxable year may include the inventory cost and adjusted basis of qualifying production property.... Accordingly, taxpayers that value inventory under § 1.471-4 (inventories at cost or market, whichever is lower... relative base-year cost method is applied with respect to all LIFO inventory of a taxpayer on a...

  20. Thermochemistry and kinetics of oxygen interaction with microporous chars. Technical report 12. [199 references

    Energy Technology Data Exchange (ETDEWEB)

    Ismail, I M.K.; Walker, P L

    1979-01-01

    In processes involving the hydrogasification of coal, the coal is first exposed to hydrogen at elevated temperatures and pressures during which devolatilization and gasification of the more reactive parts of the coal occur. A microporous coal char, which is relatively unreactive, is also produced. The char, in turn, can be reacted with steam to generate the hydrogen needed in the first part of the process. Or the char, in part, can be used in a boiler to raise steam. In any case, characterization of coal chars and the relation of character to behavior during gasification and/or combustion is important. This report is primarily concerned with laying the experimental base for the measurement of the heat of interaction of a gas (oxygen in this case) with chars. Following the laying of this base, using a char produced from a reproducible polymer system, heats of interaction of oxygen with chars produced from American coals, ranging in rank from anthracite to lignite, were measured. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) were used to study the exothermicity and kinetics of interaction between Saran chars and oxygen at selected isothermal temperatures in the range 75 to 850/sup 0/C. The combination of measuring heats of oxygen interaction with chars (using the DSC technique) and weight changes during the interaction opens up a valuable new technique to characterize coal chars. Heats of interaction at 100/sup 0/C are found to be related to rates of char gasification at higher temperatures. Both heats of interaction and gasification rates are, in turn, seen to increase sharply as the rank of coal from which the char is produced is decreased.

  1. Bifunctional chelates of RH-105 and AU199 as potential radiotherapeutic agents

    Energy Technology Data Exchange (ETDEWEB)

    Droege, P.

    1997-03-01

    Research is presented on new bifunctional chelating ligand systems with stability on the macroscopic and radiochemical levels. The synthesis of the following complexes are described: rhodium 105, palladium 109, and gold 198.

  2. Tank 241-BY-101, cores 189 and 199 analytical results for the final report

    International Nuclear Information System (INIS)

    This document is the final laboratory report for Tank 241-BY-101. Push mode core segments were removed from Pisers 10B and 10D between May 27, 1997, and June 1, 1997. Segments were received and extruded at 222-S Laboratory. Analyses were performed in accordance with Tank 241-.BY-101 Push Core Sampling and analysis Plan (TSAP) and Tank Safety Screening Data Quality Objective (DQO). None of the subsamples submitted for total alpha activity (AT) or differential scanning calorimetry (DSC) analysis exceeded the notification limits as stated in TSAP and DQO, The statistical results of the 95% confidence interval on the mean calculations are provided by the Tank Waste Remediation Systems (TWRS) Technical Basis Group, and are not considered in this report. Near Infrared Spectroscopy (NIR) analysis was requested in order to compare NIR results with those obtained from percent water gravimetry analysis (%H20) and thermal gravimetric analysis (TGA). The TWRS Technical Basis Group rescinded the request for this analysis, and neither NIR nor %H20 analyses were performed

  3. 46 CFR 199.620 - Alternatives for all vessels in a specified service.

    Science.gov (United States)

    2010-10-01

    ... pump 2 1 1 3 Boathook 1 1 1 1 1 1 4 Bucket 3 1 1 1 1 9 Fire extinguisher 1 1 1 1 1 1 12 Flashlight 1 1... boats of self-bailing design. 3 Not required for inflated or rigid-inflated rescue boats. 4 Oars...

  4. 49 CFR 192.199 - Requirements for design of pressure relief and limiting devices.

    Science.gov (United States)

    2010-10-01

    .... Except for rupture discs, each pressure relief or pressure limiting device must: (a) Be constructed of..., pipe, and fittings located between the system to be protected and the pressure relieving device,...

  5. 32 CFR 199.16 - Supplemental Health Care Program for active duty members.

    Science.gov (United States)

    2010-07-01

    ... the uniformed services to private sector health care providers for health care services provided to... supplemental health care for active duty members—(1) Hospitals covered by DRG-based payment system. For a hospital covered by the CHAMPUS DRG-based payment system to maintain its status as an authorized...

  6. 32 CFR 199.9 - Administrative remedies for fraud, abuse, and conflict of interest.

    Science.gov (United States)

    2010-07-01

    ... submitting a CHAMPUS claim for individual psychotherapy when a medical visit was the only service provided... submitting a claim for two one-hour psychotherapy sessions furnished on separate days when the actual service... notice shall include a brief statement of the nature of violations resulting in the termination...

  7. Clearance of systemic hematologic tumors by venetoclax (Abt-199) and navitoclax

    OpenAIRE

    Ackler, Scott; Oleksijew, Anatol; Chen, Jun; Chyla, Brenda J.; Clarin, Jerry; Foster, Kelly; McGonigal, Thomas; Mishra, Sasmita; Schlessinger, Sally; Smith, Morey L; Tahir, Stephen K.; Leverson, Joel D.; Souers, Andrew J.; Boghaert, Erwin R.; Hickson, Jonathan

    2015-01-01

    The Bcl-2 family inhibitors venetoclax and navitoclax demonstrated potent antitumor activity in chronic lymphocytic leukemia patients, notably in reducing marrow load and adenopathy. Subsequent trials with venetoclax have been initiated in non-Hodgkin's lymphoma and multiple myeloma patients. Traditional preclinical models fall short either in faithfully recapitulating disease progression within such compartments or in allowing the direct longitudinal analysis of systemic disease. We show tha...

  8. Clearance of systemic hematologic tumors by venetoclax (Abt-199) and navitoclax

    Science.gov (United States)

    Ackler, Scott; Oleksijew, Anatol; Chen, Jun; Chyla, Brenda J; Clarin, Jerry; Foster, Kelly; McGonigal, Thomas; Mishra, Sasmita; Schlessinger, Sally; Smith, Morey L; Tahir, Stephen K; Leverson, Joel D; Souers, Andrew J; Boghaert, Erwin R; Hickson, Jonathan

    2015-01-01

    The Bcl-2 family inhibitors venetoclax and navitoclax demonstrated potent antitumor activity in chronic lymphocytic leukemia patients, notably in reducing marrow load and adenopathy. Subsequent trials with venetoclax have been initiated in non-Hodgkin's lymphoma and multiple myeloma patients. Traditional preclinical models fall short either in faithfully recapitulating disease progression within such compartments or in allowing the direct longitudinal analysis of systemic disease. We show that intravenous inoculation of engineered RS4;11 (acute lymphoblastic leukemia) and Granta 519 (mantle cell lymphoma) bioluminescent reporter cell lines result in tumor engraftment of bone marrow, with additional invasion of the central nervous system in the case of Granta 519. Importantly, apoptosis induction and response of these systemically engrafted tumors to Bcl-2 family inhibitors alone or in combination with standard-of-care agents could be monitored longitudinally with optical imaging, and was more accurately reflective of the observed clinical response. PMID:26516589

  9. [Bifunctional chelates of Rh-105, Au-199, and other metallic radionuclides as potential radiotherapeutic agents

    Energy Technology Data Exchange (ETDEWEB)

    1991-12-31

    Progress during this period is reported under the following headings: Diethylenetriamine based and related bifunctional chelating agents and their complexation with Rh-105, Au-198, Pd-109, cu-67, In-111, and Co-57; studies of Pd-109, Rh-105 and Tc-99m with bifunctional chelates based on phenylenediamine; establishment of an appropriate protein assay method for conjugated proteins; studies of new bifunctional Bi, Tri and tetradentate amine oxime ligands with Rh-105; IgG and antibody B72.3 conjugation studies by HPLC Techniques with bifunctional metal chelates; and progress on ligand systems for Au(III).

  10. Bifunctional chelates of Rh-105 and Au-199 as potential radiotherapeutic agents

    Energy Technology Data Exchange (ETDEWEB)

    Troutner, D.E.; Schlemper, E.O.

    1987-08-31

    Since last year we have (1) Investigated the production of Rh-105 by the Szilard-Chalmers process using Ru(acac){sub 3} targets, (2) Synthesized several new ligands and their rhodium complexes, (3) Done preliminary studies of the radiochemical properties of some of these complexes of Rh-105 at 10{sup -4} -10{sup -3} M concentration, and (4) Done preliminary animal studies on one of the complexes.

  11. (Bifunctional chelates of Rh-105, Au-199, and other metallic radionuclides as potential radiotherapeutic agents)

    Energy Technology Data Exchange (ETDEWEB)

    1991-01-01

    Progress during this period is reported under the following headings: Diethylenetriamine based and related bifunctional chelating agents and their complexation with Rh-105, Au-198, Pd-109, cu-67, In-111, and Co-57; studies of Pd-109, Rh-105 and Tc-99m with bifunctional chelates based on phenylenediamine; establishment of an appropriate protein assay method for conjugated proteins; studies of new bifunctional Bi, Tri and tetradentate amine oxime ligands with Rh-105; IgG and antibody B72.3 conjugation studies by HPLC Techniques with bifunctional metal chelates; and progress on ligand systems for Au(III).

  12. Yeast Interacting Proteins Database: YNL189W, YJL199C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available tein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies...myces species; protein detected in large-scale protein-protein interaction studies Rows with this prey as pr

  13. Yeast Interacting Proteins Database: YLR291C, YJL199C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available ved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies...in large-scale protein-protein interaction studies Rows with this prey as prey Rows with this prey as prey (

  14. Yeast Interacting Proteins Database: YML064C, YJL199C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available y related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Rows wi...in-protein interaction studies Rows with this prey as prey (4) Rows with this prey as bait (1) 28 6 3 4 0 0 ...d in closely related Saccharomyces species; protein detected in large-scale prote

  15. 47 CFR 87.199 - Special requirements for 406.0-406.1 MHz ELTs.

    Science.gov (United States)

    2010-10-01

    ... is incorporated by reference in accordance with 5 U.S.C. 552(a), and 1 CFR part 51. Copies of the...-406.1 MHz ELT must contain as an integral part a homing beacon operating only on 121.500 MHz that... (a) of this section. The 121.500 MHz homing beacon must have a continuous duty cycle that may...

  16. 30 CFR 250.199 - Paperwork Reduction Act statements-information collection.

    Science.gov (United States)

    2010-07-01

    ... Prevention and Control (1010-0057) To inform MMS of measures to be taken to prevent water and air pollution. To ensure that appropriate measures are taken to prevent water and air pollution. (4) Subpart D, Oil... and regulatory requirements, will be safe and protect the human, marine, and coastal environment,...

  17. 199Hg Parameters in thymine-Hg-thymine metal mediated base pair

    Czech Academy of Sciences Publication Activity Database

    Daraku, T.; Furuita, K.; Šebera, Jakub; Yamanaka, D.; Kondo, Y.; Ono, A.; Katahira, R.; Bickelhaupt, F. M.; Sychrovský, Vladimír; Kojima, C.; Tanaka, Y.

    Rio de Janeiro : -, 2013. s. 84-84. [ISMAR Meeting /18./ - NMR Users Meeting /14./ - Iberoamerican NMR Meeting Biomedical Imaging Symposium /5./. 19.05.2013-24.05.2013, Rio de Janeiro] R&D Projects: GA ČR GAP205/10/0228 Institutional support: RVO:61388963 Keywords : NMR * T-Hg-T * J-coupling Subject RIV: CF - Physical ; Theoretical Chemistry

  18. 32 CFR 199.5 - TRICARE Extended Care Health Option (ECHO).

    Science.gov (United States)

    2010-07-01

    ... Manual of Mental Disorders” published by the American Psychiatric Association. (ii) Serious physical... Disabilities Education Act and defined at 34 CFR 300.26 and that is specifically designed to accommodate the... following its publication in the Federal Register on August 20, 2004, this section as it existed prior...

  19. Study of Neutron-Deficient $^{202-205}$Fr Isotopes with Collinear Resonance Ionization Spectroscopy

    CERN Document Server

    De Schepper, Stijn; Cocolios, Thomas; Budincevic, Ivan

    The scope of this master’s thesis is the study of neutron-deficient $^{202−205}$Fr isotopes. These isotopes are inside the neutron-deficient lead region, a region that has shown evidence of shape coexistence. For this thesis, this discussion is limited to the phenomenon where a low lying excited state has a different shape than the ground state. Shape coexistence is caused by intruder states. These are single-particle Shell Model states that are perturbed in energy due to the interaction with a deformed core. In the neutron-deficient lead region the main proton intruder orbit is the 3s$_{1/2}$orbit. When going towards more neutron-deficient isotopes, deformation increases. The $\\pi3s_{1/2}$orbit will rise in energy and will eventually become the ground state in odd- A bismuth (Z=83) isotopes. It is also observed in odd-A astatine (Z=85) isotopes, already in less neutron-deficient nuclei. The same phenomenon is expected to be present francium (Z=87) isotopes already at $^{199}$Fr. Although it is currently ...

  20. Assessment of quality of life related to health in patients with fibromyalgia - doi:10.5020/18061230.2010.p199

    OpenAIRE

    Ana Maria Martín Nogueras; Juliana Maria de Sousa Pinto; José Ignacio Calvo Arenillas

    2012-01-01

    Objectives: To determine the impact that fibromyalgia (FM) has on quality of life; to compare the quality of life of patients with fibromyalgia with the healthy Spanish population and to know the correlation and sensibility of two instruments of quality of life in FM. Method: A cross-sectional and observational study. Twenty-seven subjects joined in (25 women and 2 men) with a mean age of 50.5 years (SD 7.9), belonging to the Salmantina Association of Fibromyalgia and Chronic Fatigue. The ...

  1. Assessment of quality of life related to health in patients with fibromyalgia - doi:10.5020/18061230.2010.p199

    Directory of Open Access Journals (Sweden)

    Ana Maria Martín Nogueras

    2012-01-01

    Full Text Available Objectives: To determine the impact that fibromyalgia (FM has on quality of life; to compare the quality of life of patients with fibromyalgia with the healthy Spanish population and to know the correlation and sensibility of two instruments of quality of life in FM. Method: A cross-sectional and observational study. Twenty-seven subjects joined in (25 women and 2 men with a mean age of 50.5 years (SD 7.9, belonging to the Salmantina Association of Fibromyalgia and Chronic Fatigue. The Spanish versions of the Nottingham Health Profile (NHP and the the Short-Form 36 (SF36 were used to study quality of life. Results: High scores were obtained in the “Energy”, “Pain”, “Sleep” and “Physical mobility” dimensions of the NHP and low scores in the “Physical problems”, “Pain”, “Vitality” and “General health perception” dimensions of the SF-36. Statistical significant differences were found in all dimensions comparing to the healthy Spanish population. The following dimensions were negatively statistically correlated: “Energy”(NHP-“Vitality” (SF-36; “Pain”(NHP- “Physical function”, “Pain and Vitality” (SF-36; “Physical mobility” (NHP-“Physical function and Physical problems” (SF-36; “Emotional reactions” (NHP- “Mental health”, “Emotional problems” and “General health” (SF-36. The SF-36 is more sensitive than the NHP to evaluate the deterioration of the fibromyalgia. Conclusions: The fibromyalgia produces a significant deterioration in quality of life that can be assessed by the dimensions of the NHP and the SF-36.

  2. A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae.

    Science.gov (United States)

    Adeboye, Peter Temitope; Olsson, Lisbeth; Bettiga, Maurizio

    2016-07-01

    The conversion of coniferyl aldehyde to cinnamic acids by Saccharomyces cerevisiae under aerobic growth conditions was previously observed. Bacteria such as Pseudomonas have been shown to harbor specialized enzymes for converting coniferyl aldehyde but no comparable enzymes have been identified in S. cerevisiae. CALDH from Pseudomonas was expressed in S. cerevisiae. An acetaldehyde dehydrogenase (Ald5) was also hypothesized to be actively involved in the conversion of coniferyl aldehyde under aerobic growth conditions in S. cerevisiae. In a second S. cerevisiae strain, the acetaldehyde dehydrogenase (ALD5) was deleted. A prototrophic control strain was also engineered. The engineered S. cerevisiae strains were cultivated in the presence of 1.1mM coniferyl aldehyde under aerobic condition in bioreactors. The results confirmed that expression of CALDH increased endogenous conversion of coniferyl aldehyde in S. cerevisiae and ALD5 is actively involved with the conversion of coniferyl aldehyde in S. cerevisiae. PMID:27070284

  3. ERBE Wide-Field-of-View Nonscanner Data Reprocessing and revisiting its Radiation dataset from 1985 to 199

    Science.gov (United States)

    Shrestha, A. K.; Kato, S.; Wong, T.; Stackhouse, P. W.; Rose, F. G.; Miller, W. F.; Bush, K.; Rutan, D. A.; Minnis, P.; Doelling, D.

    2015-12-01

    The Earth's radiation budget is a fundamental component of the climate system and should reflect the variation in climate. As such, it is critical to know how it has varied over past decades to ensure that climate models are properly representing climate. Broadband shortwave and longwave irradiances were measured by the Earth Radiation Budget Experiment (ERBE) wide-field-of-view (WFOV) nonscanner instrument from 1985 to 1998. These WFOV nonscanner instruments were onboard NASA's Earth Radiation Budget Satellite (ERBS) and two NOAA's satellites (NOAA-9 and NOAA-10). However, earlier studies showed that the transmissivity of the dome for the WFOV shortwave (SW) nonscanner instrument degraded over time. To account for the degradation, WFOV instruments were calibrated assuming constant spectral degradation (gray assumption). Recent developments from analysis of data from the Clouds and the Earth's Radiant Energy System project (CERES), which has been measuring the radiation budget since 2000, suggest that transmissivity of shorter wavelength degrades faster. Therefore, a spectrally dependent degradation correction is needed for a better calibration. In addition, accounting for the spectrally dependent degradation may eliminate an additional correction applied to irradiances using a time series of daytime and nighttime longwave irradiance differences. Therefore, we have reprocessed WFOV nonscanner data by characterizing the spectrally dependent degradation of the SW dome transmissivity. Time and spectral dependent degradation of the shortwave filter function is estimated using solar data observed by these instruments during calibration days. Because the spectrum of reflected irradiance depends on scene type, we use Advanced Very High Resolution Radiometer AVHRR-derived cloud properties and surface type over the WFOV footprints in addition to time dependent filter function for the unfiltering process. This poster explains the reprocessing approach and discusses the results by comparing the existing ERBE radiation dataset to that obtained with reprocessing using the new correction. This leads to the generation of a long-term radiation datasets consistent with those provided by CERES.

  4. SU-E-J-199: A Software Tool for Quality Assurance of Online Replanning with MR-Linac

    International Nuclear Information System (INIS)

    Purpose: To develop a quality assurance software tool, ArtQA, capable of automatically checking radiation treatment plan parameters, verifying plan data transfer from treatment planning system (TPS) to record and verify (R&V) system, performing a secondary MU calculation considering the effect of magnetic field from MR-Linac, and verifying the delivery and plan consistency, for online replanning. Methods: ArtQA was developed by creating interfaces to TPS (e.g., Monaco, Elekta), R&V system (Mosaiq, Elekta), and secondary MU calculation system. The tool obtains plan parameters from the TPS via direct file reading, and retrieves plan data both transferred from TPS and recorded during the actual delivery in the R&V system database via open database connectivity and structured query language. By comparing beam/plan datasets in different systems, ArtQA detects and outputs discrepancies between TPS, R&V system and secondary MU calculation system, and delivery. To consider the effect of 1.5T transverse magnetic field from MR-Linac in the secondary MU calculation, a method based on modified Clarkson integration algorithm was developed and tested for a series of clinical situations. Results: ArtQA is capable of automatically checking plan integrity and logic consistency, detecting plan data transfer errors, performing secondary MU calculations with or without a transverse magnetic field, and verifying treatment delivery. The tool is efficient and effective for pre- and post-treatment QA checks of all available treatment parameters that may be impractical with the commonly-used visual inspection. Conclusion: The software tool ArtQA can be used for quick and automatic pre- and post-treatment QA check, eliminating human error associated with visual inspection. While this tool is developed for online replanning to be used on MR-Linac, where the QA needs to be performed rapidly as the patient is lying on the table waiting for the treatment, ArtQA can be used as a general QA tool in radiation oncology practice. This work is partially supported by Elekta Inc

  5. Fully relativistic coupled cluster and DFT study of electric field gradients at Hg in 199Hg compounds

    DEFF Research Database (Denmark)

    Arcisauskaité, Vaida; Knecht, Stefan; Sauer, Stephan P. A.;

    2012-01-01

    We investigate the magnitude and interplay of relativistic and electron correlation effects on the electric field gradient (EFG) at the position of Hg in linear and bent HgL2 (L=CH3, Cl, Br, I) and trigonal planar [HgCl3]- complexes using four-component relativistic Dirac-Coulomb (DC) and non...

  6. The Progression of Liver Fibrosis Is Related with Overexpression of the miR-199 and 200 Families

    OpenAIRE

    Murakami, Yoshiki; Toyoda, Hidenori; Tanaka, Masami; Kuroda, Masahiko; Harada, Yoshinori; Matsuda, Fumihiko; Tajima, Atsushi; Kosaka, Nobuyoshi; Ochiya, Takahiro; Shimotohno, Kunitada

    2011-01-01

    Background Chronic hepatitis C (CH) can develop into liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Liver fibrosis and HCC development are strongly correlated, but there is no effective treatment against fibrosis because the critical mechanism of progression of liver fibrosis is not fully understood. microRNAs (miRNAs) are now essential to the molecular mechanisms of several biological processes. In order to clarify how the aberrant expression of miRNAs participates in development o...

  7. 1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions

    Directory of Open Access Journals (Sweden)

    Mateusz Wojtusik

    2015-06-01

    Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 °C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained.

  8. 15 CFR 19.9 - When will Commerce entities transfer a Commerce debt to the Treasury Department's Financial...

    Science.gov (United States)

    2010-01-01

    ... services, a process known as “cross-servicing.” See 31 U.S.C. 3711(g) and 31 CFR 285.12. Commerce entities... to the Commerce debt and the collection action to be taken. See 31 CFR 285.12(b) and 285.12(c)(2... accordance with the procedures described in 31 CFR 285.12. The Financial Management Service takes...

  9. Nuclear magnetic resonance shielding constants and chemical shifts in linear 199Hg compounds: a comparison of three relativistic computational methods.

    Science.gov (United States)

    Arcisauskaite, Vaida; Melo, Juan I; Hemmingsen, Lars; Sauer, Stephan P A

    2011-07-28

    We investigate the importance of relativistic effects on NMR shielding constants and chemical shifts of linear HgL(2) (L = Cl, Br, I, CH(3)) compounds using three different relativistic methods: the fully relativistic four-component approach and the two-component approximations, linear response elimination of small component (LR-ESC) and zeroth-order regular approximation (ZORA). LR-ESC reproduces successfully the four-component results for the C shielding constant in Hg(CH(3))(2) within 6 ppm, but fails to reproduce the Hg shielding constants and chemical shifts. The latter is mainly due to an underestimation of the change in spin-orbit contribution. Even though ZORA underestimates the absolute Hg NMR shielding constants by ∼2100 ppm, the differences between Hg chemical shift values obtained using ZORA and the four-component approach without spin-density contribution to the exchange-correlation (XC) kernel are less than 60 ppm for all compounds using three different functionals, BP86, B3LYP, and PBE0. However, larger deviations (up to 366 ppm) occur for Hg chemical shifts in HgBr(2) and HgI(2) when ZORA results are compared with four-component calculations with non-collinear spin-density contribution to the XC kernel. For the ZORA calculations it is necessary to use large basis sets (QZ4P) and the TZ2P basis set may give errors of ∼500 ppm for the Hg chemical shifts, despite deceivingly good agreement with experimental data. A Gaussian nucleus model for the Coulomb potential reduces the Hg shielding constants by ∼100-500 ppm and the Hg chemical shifts by 1-143 ppm compared to the point nucleus model depending on the atomic number Z of the coordinating atom and the level of theory. The effect on the shielding constants of the lighter nuclei (C, Cl, Br, I) is, however, negligible. PMID:21806118

  10. Study of the decay K+ => pi+,nu,nubar in the momentum region 140199 MeV/c

    CERN Document Server

    Artamonov, A V; Bhuyan, B; Blackmore, E W; Bryman, D A; Chen, S; Chiang, I-H; Christidi, I -A; Cooper, P S; Diwan, M V; Frank, J S; Fujiwara, T; Hu, J; Ives, J; Jaffe, D E; Kabe, S; Kettell, S H; Khabibullin, M M; Khotjantsev, A N; Kitching, P; Kobayashi, M; Komatsubara, T K; Konaka, A; Kozhevnikov, A P; Kudenko, Yu G; Kushnirenko, A; Landsberg, L G; Lewis, B; Li, K K; Littenberg, L S; MacDonald, J A; Mildenberger, J; Mineev, O V; Miyajima, M; Mizouchi, K; Mukhin, V A; Muramatsu, N; Nakano, T; Nomachi, M; Nomura, T; Numao, T; Obraztsov, V F; Omata, K; Patalakha, D I; Petrenko, S V; Poutissou, R; Ramberg, E J; Redlinger, G; Sato, T; Sekiguchi, T; Shinkawa, T; Strand, R C; Sugimoto, S; Tamagawa, Y; Tschirhart, R; Tsunemi, T; Vavilov, D V; Viren, B; Wang, Zhe; Yershov, N V; Yoshimura, Y; Yoshioka, T

    2009-01-01

    Experiment E949 at Brookhaven National Laboratory has observed three new events consistent with the decay K+ => pi+,nu,nubar in the pion momentum region 140 pi+,nu,nubar events to seven. Combining this observation with previous results, assuming the pion spectrum predicted by the standard model, results in a branching ratio of (1.73+1.15-1.05)e-10. An interpretation of the results for alternative models of the decay K^ => pi+,nothing is also presented.

  11. Study of the decay K+ => pi+,nu,nubar in the momentum region 140199 MeV/c

    OpenAIRE

    E791 Collaboration; Artamonov, A. V.; Bassalleck, B.; Bhuyan, B.; Blackmore, E. W.; Bryman, D. A.; Chen, S.; Chiang, I-H.; Christidi, I. -A.; Cooper, P.S.; Diwan, M. V.; Frank, J. S.; T. Fujiwara; Hu, J; Ives, J

    2009-01-01

    Experiment E949 at Brookhaven National Laboratory has observed three new events consistent with the decay K+ => pi+,nu,nubar in the pion momentum region 140 pi+,nu,nubar events to seven. Combining this observation with previous results, assuming the pion spectrum predicted by the standard model, results in a ...

  12. «Cantar de Mio Cid», vv. 1-99. Prove di una nuova edizione

    Directory of Open Access Journals (Sweden)

    Alfonso D'Agostino

    2014-07-01

    Full Text Available In vista di una nuova edizione del Cantar de Mio Cid, l’autore propone uno specimen dei primi 99 versi dell’opera, pubblicati in una doppia edizione sinottica: un testo interpretativo e una ricostruzione critica. Il primo è fedele al manoscritto unico, limitandosi a correggere le sviste del copista; la seconda si basa sulla teoria metrica già illustrata dall’autore in altri saggi, ma fa appello anche alla tradizione indiretta, all’usus scribendi, alla conformatio textus e alla logica interna del racconto. Il testo è corredato da note filologiche. As part of his work on a new edition of Cantar de mio Cid, the author presents a sample of the first 99 verses of the poem in a twofold synoptic version, with an interpretative text and a critical reconstruction. The first is true to the single manuscript and only corrects the copyist’s mistakes. The latter is based on the metric theory that was explained by the author in other essays, enriched by the resort to indirect tradition, usus scribendi, conformatio textus and the plot’s inner logic. The text is accompanied by philological notes. 

  13. The extinction curve of the lensing galaxy of $B1152+199$ at $z=0.44$

    CERN Document Server

    Toft, S; Burud, I; Toft, Sune; Hjorth, Jens; Burud, Ingunn

    2000-01-01

    We present UBVRIz' photometry of the gravitational lens candidate CLASSB1152+119 obtained with the Nordic Optical Telescope. The two QSO componentsare resolved in the B, V, R, I and z' bands confirming the lensing nature ofthe system. The z=0.44 lens galaxy is clearly detected in B, R, I and z' andits position is found to be almost coincident with the faint QSO image which isheavily extincted (relative to the brighter QSO image) by dust in the lensgalaxy. The extinction curve of the lens galaxy derived from the relativephotometry is well fitted by a Galactic extinction law with 1.3 < R_V < 2.0 andE(B-V) ~ 1. From a simple model of the system we predict a time delay of ~ 60days.

  14. Speciation of Aquatic Heavy Metals in Humic Substances by$^{111m}$Cd/$^{199m}$Hg-TDPAC

    CERN Multimedia

    2002-01-01

    Humic substances are ubiquitous in waters and soils and act as complexing agents for different heavy metals, e.g. Cd, Hg. Toxicity, reactivity, fixation and migration are therefore strongly influenced by the interactions between heavy metals and humic substances. Humic substances derive from postmortal materials such as rotten plants, have dark colours and usually a molecular weight between 500 and 10~000 Dalton. Complex formation studies with different heavy metal ions indicate at least two different kinds of metal sites. Usually, these studies are restricted to heavy metal concentrations 2 to 3 orders of magnitude higher than the natural heavy metal abundance (i.e. 10$^{-10}$ molar). This serious limitation can be overcome by the use of suitable radiosotope techniques capable of metal speciation at extreme sensitivity levels such as TDPAC (Time Differential Perturbed Angular Correlation of~$\\gamma$-rays). Thus, we studied the interaction of heavy metals with humic substances by monitoring the nuclear quadru...

  15. Diagnostic and Prognostic Impact of Circulating YKL-40, IL-6, and CA 19.9 in Patients with Pancreatic Cancer

    DEFF Research Database (Denmark)

    Schultz, Nicolai A; Christensen, Ib J; Werner, Jens;

    2013-01-01

    We tested the hypothesis that high plasma YKL-40 and IL-6 associate with pancreatic cancer and short overall survival.......We tested the hypothesis that high plasma YKL-40 and IL-6 associate with pancreatic cancer and short overall survival....

  16. SU-E-J-199: Evaluation of Motion Tracking Effects On Stereotactic Body Radiotherapy of Abdominal Targets

    Energy Technology Data Exchange (ETDEWEB)

    Monterroso, M; Dogan, N; Yang, Y [University Miami, Miami, FL (United States)

    2014-06-01

    Purpose: To evaluate the effects of respiratory motion on the delivered dose distribution of CyberKnife motion tracking-based stereotactic body radiotherapy (SBRT) of abdominal targets. Methods: Four patients (two pancreas and two liver, and all with 4DCT scans) were retrospectively evaluated. A plan (3D plan) using CyberKnife Synchrony was optimized on the end-exhale phase in the CyberKnife's MultiPlan treatment planning system (TPS), with 40Gy prescribed in 5 fractions. A 4D plan was then created following the 4D planning utility in the MultiPlan TPS, by recalculating dose from the 3D plan beams on all 4DCT phases, with the same prescribed isodose line. The other seven phases of the 4DCT were then deformably registered to the end-exhale phase for 4D dose summation. Doses to the target and organs at risk (OAR) were compared between 3D and 4D plans for each patient. The mean and maximum doses to duodenum, liver, spinal cord and kidneys, and doses to 5cc of duodenum, 700cc of liver, 0.25cc of spinal cord and 200cc of kidneys were used. Results: Target coverage in the 4D plans was about 1% higher for two patients and about 9% lower in the other two. OAR dose differences between 3D and 4D varied among structures, with doses as much as 8.26Gy lower or as much as 5.41Gy higher observed in the 4D plans. Conclusion: The delivered dose can be significantly different from the planned dose for both the target and OAR close to the target, which is caused by the relative geometry change while the beams chase the moving target. Studies will be performed on more patients in the future. The differences of motion tracking versus passive motion management with the use of internal target volumes will also be investigated.

  17. Synthesis of LiSm_(0.01)Mn_(1.99)O_4 by molten salt technique

    Institute of Scientific and Technical Information of China (English)

    M; Helan; L; John; Berchmans

    2010-01-01

    Samarium substituted lithium manganese oxide powders were successfully prepared by molten-salt synthesis(MSS) using eutectic mixture of LiCl,SmCl3.6H2O and MnO2 salt at 700 °C.The synthesis was carried out in open atmosphere.The crystalline powders were characterized for their phase identification using X-ray diffraction(XRD) analysis.The physico-chemical properties of the samarium substituted lithium manganese oxide powders were investigated by thermal analysis(TGA/DTA),FT-IR spectroscopy,EDAX,electron par...

  18. Shelf-Life of ɛ-Lysyl-3-(Trimethylstannyl)Benzamide Immunoconjugates, Precursors for 211At Labeling of Antibodies

    OpenAIRE

    Aneheim, Emma; Halleröd, Jenny; Albertsson, Per; Jensen, Holger; Holgersson, Stellan; Lindegren, Sture

    2015-01-01

    Astatine-211 is possibly the most promising radionuclide for targeted α-particle therapy when it comes to the treatment of occult disseminated cancer. Preclinical research has proven effective, and patient studies have been initiated based on these results. However, a lack of production capacity and the complex radiochemistry of 211At are major obstacles for research and prospective clinical applications. In the present study, astatination of immunoconjugates, already prepared well in advance...

  19. Postvaccination increase in serotype 19A pneumococcal disease in Norway is driven by expansion of penicillin-susceptible strains of the ST199 complex.

    Science.gov (United States)

    Vestrheim, Didrik F; Steinbakk, Martin; Aaberge, Ingeborg S; Caugant, Dominique A

    2012-03-01

    Serotype replacement in invasive pneumococcal disease has been observed after widespread use of the 7-valent pneumococcal conjugate vaccine (PCV7). Replacement is dominated by penicillin-nonsusceptible serotype 19A in several countries. Antibiotic selection pressure has been proposed to interact with immunization, leading to rapid replacement. In Norway, where prescription of antibiotics is limited, post-PCV7 replacement by serotype 19A is dominated by penicillin-susceptible clones. Hence, serotype 19A replacement occurs, although it is not driven by antibiotic selection pressure. PMID:22237889

  20. Postvaccination Increase in Serotype 19A Pneumococcal Disease in Norway Is Driven by Expansion of Penicillin-Susceptible Strains of the ST199 Complex

    OpenAIRE

    Vestrheim, Didrik F.; Steinbakk, Martin; Aaberge, Ingeborg S; Dominique A. Caugant

    2012-01-01

    Serotype replacement in invasive pneumococcal disease has been observed after widespread use of the 7-valent pneumococcal conjugate vaccine (PCV7). Replacement is dominated by penicillin-nonsusceptible serotype 19A in several countries. Antibiotic selection pressure has been proposed to interact with immunization, leading to rapid replacement. In Norway, where prescription of antibiotics is limited, post-PCV7 replacement by serotype 19A is dominated by penicillin-susceptible clones. Hence, se...

  1. Expression of miR-199a, lum and ets2 in benthic and pelagic Arctic charr morphs at developmental stage 200τ

    OpenAIRE

    Ligthart, Kate, 1992-

    2014-01-01

    MiRNAs play an important role in development by regulating gene expression. They are recruited to RISC complexes, which bind to target mRNAs. This interaction causes silencing of the target mRNAs by repressing translation or by initiating degradation. In lake Þingvallavatn there are four morphs of Arctic charr that are very different in morphology and their feeding apparatus. The different morphs of Arctic charr have different levels of miRNA gene expression during their embryonic development...

  2. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

    OpenAIRE

    Yang, Kai

    2016-01-01

    Chuanshu Yuan, Kai Yang, Hong Tang, Dan Chen Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China Background: At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SC...

  3. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

    OpenAIRE

    Yuan CS; Yang K; Tang H; Chen D

    2016-01-01

    Chuanshu Yuan, Kai Yang, Hong Tang, Dan Chen Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China Background: At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SCCAg)...

  4. Discrimination of two geographically distinct populations of sea bass Dicentrarchus labrax, using stable isotopic signatures of mercury (δ202Hg, Δ199Hg)

    OpenAIRE

    Cransveld, Alice; Amouroux, David; Koutrakis, Emmanuil; Bérail, Sylvain; Caiyan, Feng; Barre, Julien P. G.; Schnitzler, Joseph; Das, Krishna

    2014-01-01

    Despite the reduction of mercury (Hg) emissions in Europe in the last decades, Hg emissions are increasing worldwide and concentrations found in some marine predators remain high. This raises questions on mercury's biogeochemical cycle at both local and global scale. In the present work, we investigate the possibility to use mercury isotopic signature in fish as a tool to discriminate different polluted areas and potential pollution sources. Indeed, Hg can exhibit both mass-dependent (MDF) an...

  5. Køn til forhandling - køb en krop i Fitness World for 199,- om måneden

    OpenAIRE

    Hansen, Louise Mie Dunk; Jacobsen, Sebastian Schlüter; Sørensen, Simon Baastrup; Knudsen, Frederikke Johansen; Koueik, Rita Mourched; Fuhlendorff, Cecilie Lundskov

    2016-01-01

    This study investigates how Fitness World and their members portray gender and body images based on social constructivism in the postmodern society. The baseline for the scientific theory is Michel Foucault’s approach to gender with a further construction of Judith Butler’s gender theory. The empiricism is mainly based on qualitative interviews with six members from Fitness World Lyongade, Amager. Moreover the empiricism also includes Fitness World’s commercials, quotes and their overall p...

  6. Phosphorylation of the tau protein sequence 199-205 in the hippocampal CA3 region of Syrian hamsters in adulthood and during aging

    NARCIS (Netherlands)

    Hartig, W; Oklejewicz, M; Strijkstra, AM; Boerema, AS; Stieler, J; Arendt, T

    2005-01-01

    Paired helical filaments formed by the abnormally phosphorylated microtubule-associated tau are a main sign of Alzheimer's disease and other neurodegenerative disorders. The hippocampal CA3 region, a brain region with a high degree of synaptic plasticity, is known to be strongly involved in tau hype

  7. Safety Evaluation Report related to the renewal of the operating license for the research reactor at Manhattan College (Docket No. 50-199)

    International Nuclear Information System (INIS)

    This Safety Evaluation Report for the application filed by Manhattan College (MC) for a renewal of Operating License R-94 to continue to operate the MC 0.1-W open-pool training reactor has been prepared by the Office of Nuclear Reactor Regulation of the US Nuclear Regulatory Commission. The facility is owned and operated by MC and is located two blocks away from the MC main campus in the Riverdale area of New York City, New York. The staff concludes that the reactor facility can continue to be operated by MC without endangering the health and safety of the public

  8. Use of tagged random hexamer amplification (TRHA) to clone and sequence minute quantities of DNA--application to a 180 kb plasmid isolated from Sphingomonas F199.

    OpenAIRE

    Wong, K.K.; Stillwell, L C; Dockery, C A; Saffer, J D

    1996-01-01

    We have developed a novel method to clone and sequence minute quantities of DNA. The method was applied to sequence a 180 kb plasmid pNL1. The first step was the production of a size distributed population of DNA molecules that were derived from the 180 kb plasmid pNL1. The first step was accomplished by a random synthesis reaction using Klenow fragment and random hexamers tagged with a T7 primer at the primer 5'-end (T7-dN6, 5'-GTAATACGACTCACTATAGGGCNNNNNN-3'. In the second step, Klenow-synt...

  9. Direct detection of the mercury-nitrogen bond in the thymine-Hg-II-thymine base-pair with Hg-199 NMR spectroscopy

    Czech Academy of Sciences Publication Activity Database

    Dairaku, T.; Furuita, K.; Sato, H.; Šebera, Jakub; Yamanaka, D.; Otaki, H.; Kikkawa, S.; Kondo, Y.; Katahira, R.; Bickelhaupt, F. M.; Guerra, C. F.; Ono, A.; Sychrovský, Vladimír; Kojima, C.; Tanaka, Y.

    2015-01-01

    Roč. 51, č. 40 (2015), s. 8488-8491. ISSN 1359-7345 R&D Projects: GA ČR GAP205/10/0228 Institutional support: RVO:61388963 Keywords : T-Hg-T * NMR * spin-spin coupling * DFT Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 6.834, year: 2014 http://pubs.rsc.org/en/content/articlepdf/2015/cc/c5cc02423d

  10. fRNAdb Summary: FR282481 [

    Lifescience Database Archive (English)

    Full Text Available FR282481 AJ560739 mature micro RNA (miRNA) miR-199* / miR-199-3p / miR-199-as / miR-199-star ... / m ... iR-199a* / miR-199a-3p / miR-199a-as / miR-199a-star ... miRNA Danio rerio,Xenopus tropicalis,Homo sapiens, ...

  11. 血清CEA、CA19-9及铁蛋白联合检测在胰腺癌诊断中的价值%Value of combination detection of serum CEA,CA19-9 and ferritin in diagnosing pancreatic cancer

    Institute of Scientific and Technical Information of China (English)

    何淑霞; 杨娜; 刘东红

    2015-01-01

    目的:探究血清癌胚抗原(CEA)、糖类抗原19‐9(CA19‐9)及铁蛋白联合检测在胰腺癌诊断中的价值。方法选取2011年7月至2013年4月在漯河市中医院住院治疗且确诊的胰腺癌患者64例(胰腺癌组),另选取慢性胰腺炎患者36例(慢性胰腺炎组)及体检健康者42例(对照组),比较各组血清CEA、CA19‐9及铁蛋白水平及其检测敏感性和特异性。结果胰腺癌患者血清CEA、CA19‐9及铁蛋白的表达水平均高于慢性胰腺炎组和对照组,差异均有统计学意义(P<0.05);不同TNM分期胰腺癌患者血清CEA、CA19‐9及铁蛋白表达水平比较差异均有统计学意义(P<0.05);生存期不你Ⅲ6个月的患者血清CEA、CA19‐9及铁蛋白表达水平均低于生存期少于6个月的患者,比较差异均有统计学意义(P<0.05);血清CEA、CA19‐9及铁蛋白联合检测的敏感性为87.2%,特异性为96.1%。结论血清CEA、CA19‐9及铁蛋白联合检测对胰腺癌的诊断有着重要的参考价值。%Objective To investigate the value of the combination detection of serum CEA ,CA19‐9 and ferritin in the diagnosis of pancreatic cancer .Methods 64 patients conducted pancreatic cancer(pancreatic caner group) ,oth‐er 36 patients with chronic pancreatitis (chronic pancreatitis group) and 42 healthy persons conducted physical exam‐ination(control group) in our hospital from Jul .2011 to Apr .2013 were recruited for this study .Serum CEA ,CA19‐9 and ferritin expression levels in each group and detection sensitivity and specificity were compared .Results The ser‐um CEA ,CA19‐9 and ferritin expression levels in the pancreatic cancer group were significantly higher than those in the chronic pancreatitis group and the control group ,the differences had statistical significance(P<0 .05);the serum CEA ,CA19‐9 and ferritin expression levels had the statistical differences among different stages of TNM classifica‐tion (P<0 .05);the serum CEA ,CA19‐9 and ferritin expression levels in the patients with the survival period ≥6 months were lower than those in the patients with the survival period<6 months ,the differences had statistical sig‐nificance(P<0 .05);the sensitivity and the specificity of the combination detection of CEA ,CA19‐9 and ferritin were 87 .2% and 96 .1% respectively .Conclusion The combination detection of serum CEA ,CA19‐9 and ferritin could have important reference value for the diagnosis of pancreatic cancer .

  12. Shelf-life of ɛ-lysyl-3-(trimethylstannyl)benzamide immunoconjugates, precursors for 211At labeling of antibodies

    DEFF Research Database (Denmark)

    Aneheim, Emma; Halleröd, Jenny; Albertsson, Per;

    2015-01-01

    Astatine-211 is possibly the most promising radionuclide for targeted α-particle therapy when it comes to the treatment of occult disseminated cancer. Preclinical research has proven effective, and patient studies have been initiated based on these results. However, a lack of production capacity ...

  13. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    Energy Technology Data Exchange (ETDEWEB)

    Jaszczak, R.J.

    1995-12-01

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons.

  14. SPECT assay of radiolabeled monoclonal antibodies. Final performance report, March 1992--November 1995

    International Nuclear Information System (INIS)

    Research is described in the following areas: development and evaluation quantitatively of reconstruction algorithms with improved compensations for attenuation, scatter, and geometric collimator response; evaluation of single photon emission computed tomography (SPECT) quantification of iodine 123 and astatine 211; and the development and evaluation of SPECT pinhole imaging for low and medium energy photons

  15. Separation of 211At from irradiated bismuth target and labelling of a model protein

    International Nuclear Information System (INIS)

    Astatine-211 is a promising radionuclide for therapeutic use in nuclear medicine. An efficient method (70%) for radiochemical separation from the irradiated Bi-target has been described. The level of Bi contamination in the purified 2'11At-solution was estimated. Finally labelling of a model protein has been tried. (author). 14 refs

  16. Report by the parliamentary mission on nuclear safety, the role and future of the nuclear sector - Final report: the future of the nuclear sector in France - National Assembly report nr 4097, Senate report nr 199

    International Nuclear Information System (INIS)

    This large report first outlines the fact that the French nuclear sector is adapted to the national context while discussing the difference with other countries and the French strategic priorities. It describes the constraints another approach would have regarding the objective of a controlled consumption, the exploitation of renewable energies, and the remanence of the burden related to nuclear energy (culture of safety, the waste management issue). It proposes a prospective vision for the nuclear sector with respect with technological perspectives, and analyses three scenarios. The document also contains reports of visits in Germany and in Fukushima. A second part proposes the contents of hearings and meetings, as well as reports of other visits on French nuclear sites

  17. Multibeam collection for KN199-02: Multibeam data collected aboard Knorr from 2010-09-07 to 2010-09-29, departing from Nuuk (Godthab), Greenland and returning to Nuuk (Godthab), Greenland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  18. Niskin bottle data of the Hawaii Ocean Time-series (HOT) program in the North Pacific 100 Miles North of Oahu, Hawaii for Cruises HOT199-206 during 2008 (NODC Accession 0059936)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The HOT program makes repeated observations of the physics, biology and chemistry at a site approximately 100 km north of Oahu, Hawaii. Two stations are visited...

  19. Thermosalinograph data of the Hawaii Ocean Time-series (HOT) program in the North Pacific 100 Miles North of Oahu, Hawaii for Cruises HOT199-206 during 2008 (NODC Accession 0059943)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The HOT program makes repeated observations of the physics, biology and chemistry at a site approximately 100 km north of Oahu, Hawaii. Two stations are visited...

  20. Oana Salişteanu Cristea, Prestito latino - Elemento ereditario nel lessico della lingua italiana - Doppioni e varianti, Istituto di Studi Romanzi, Facoltà di Lettere, Università Carolina Praga; Praga 2000, pp. 199

    Directory of Open Access Journals (Sweden)

    Pavao Tekavčić

    2005-12-01

    Full Text Available La ricchezza del lessico italiano dall'antichità ad oggi e la sua complicata stratificazione sono oggetto dell'interesse dei linguisti da più di un secolo e mezzo; eppure, c'è una serie di problemi non studiati a fondo, tuttora aperti e promettenti. Uno di tali temi è la coesistenza di due (o più riflessi di una sola base latina, cioè gli allotropi e doppioni (franc. doublets. Dai tempi di Ugo Angelo Canello (anni 70 dell'Ottocento questo settore del vocabolario non cessa di preoccupare gli studiosi ed il più recente contributo -- importantissimo, diciamolo subito-- che riassume, discute, sistematizza e in gran parte completa quanto fatto finora, è il recentissimo volume della professoressa Oana Sălişteanu Cristea, docente di linguistica italiana (storia della lingua, filologia e dialettologia all'Università di Bucarest. Questo libro è l'oggetto della presente recensione.

  1. Corrigendum to “Robust error bounds for finite element approximation of reaction–diffusion problems with non-constant reaction coefficient in arbitrary space dimension” [Comput. Methods Appl. Mech. Engrg. 281 (2014) 184–199

    Czech Academy of Sciences Publication Activity Database

    Ainsworth, M.; Vejchodský, Tomáš

    2016-01-01

    Roč. 299, February (2016), s. 143. ISSN 0045-7825 Institutional support: RVO:67985921 Subject RIV: BA - General Mathematics Impact factor: 2.959, year: 2014 http://www.sciencedirect.com/science/article/pii/S0045782515003473

  2. CTD data of the Hawaii Ocean Time-series (HOT) program in the North Pacific 100 Miles North of Oahu, Hawaii for Cruises HOT199-206 during 2008 (NODC Accession 0059842)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The HOT program makes repeated observations of the physics, biology and chemistry at a site approximately 100 km north of Oahu, Hawaii. Two stations are visited...

  3. A seismic-reflection investigation of gas hydrates and sea-floor features of the upper continental slope of the Garden Banks and Green Canyon regions, northern Gulf of Mexico: report for cruise G1-99-GM (99002)

    Science.gov (United States)

    Cooper, Alan; Twichell, David; Hart, Patrick

    1999-01-01

    During April 1999, the U.S. Geological Survey (USGS) conducted a 13-day cruise in the Garden Banks and Green Canyon regions of the Gulf of Mexico. The R/V Gyre, owned by Texas A&M University, was chartered for the cruise. The general objectives were (1) to acquire very high resolution seismic-reflection data and side-scan sonar images of the upper and middle continental slope (200-1200-m water depths), (2) to study the acoustic character and features of the sea floor for evidence of sea-floor hazards, and (3) to look for evidence of subsurface gas hydrates and their effects. The Gulf of Mexico is well known for hydrocarbon resources, with emphasis now on frontier deep-water areas. For water depths greater than about 250 m, the pressure-termperature conditions are correct for the development of shallow-subsurface gas hydrate formation (Anderson et al., 1992). Gas hydrates are ice-like mixtures of gas and water (Kvenvolden, 1993). They are known to be present from extensive previous sampling in sea-floor cores and from mound-like features observed on the sea floor in many parts of the northern Gulf, including the Green Canyon and Garden Banks areas (e.g., Roberts, 1995). Seismic-reflection data are extensive in the Gulf of Mexico, but few very-high-resolution data like those needed for gas-hydrate studies exist in the public domain. The occurrence and mechanisms of gas hydrate formation and dissociation are important to understand, because of their perceived economic potential for methane gas, their potential controls on local and regional sea-floor stability, and their possible effects on earth climates due to massive release of methane greenhouse gas into the atmosphere. Three high-resolution seismic-reflection systems and one side-scan sonar system were used on the cruise to map the surface reflectance and features of the sea floor and the acoustic geometries and character of the shallow sub-surface. The cruise was designed to acquire regional and detailed local information. The regional survey covered an area about 3400 km2 in the Green Canyon and Garden Banks regions. Data recorded included 15 cu. in. water gun multichannel seismic-reflection and Huntec boomer information. Detailed surveys were planned in two parts of the study area, but due to a winch failure only one detailed survey was done in the Green Canyon area. The detailed survey included collection of 15 cu. in. water gun multichannel seismic-reflection, chirp seismic-reflection, and side-scan data.

  4. Water Column Chemical Data of the Hawaii Ocean Time-series (HOT) program in the North Pacific 100 Miles North of Oahu, Hawaii for Cruises HOT199-227 during 2008-2010 (NCEI Accession 0088839)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The HOT program makes repeated observations of the physics, biology and chemistry at a site approximately 100 km north of Oahu, Hawaii. Two stations are visited...

  5. Multibeam collection for KN199-03: Multibeam data collected aboard Knorr from 2010-10-01 to 2010-10-10, departing from Nuuk (Godthab), Greenland and returning to Lisboa, Portugal

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  6. Multibeam collection for KN199-04: Multibeam data collected aboard Knorr from 2010-10-15 to 2010-11-04, departing from Lisbon, Portugal and returning to Porto Grande, Cape Verde

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  7. Phase 1-2a multicenter dose-escalation study of ezatiostat hydrochloride liposomes for injection (Telintra®, TLK199, a novel glutathione analog prodrug in patients with myelodysplastic syndrome

    Directory of Open Access Journals (Sweden)

    Burris Howard

    2009-05-01

    Full Text Available Abstract Background Ezatiostat hydrochloride liposomes for injection, a glutathione S-transferase P1-1 inhibitor, was evaluated in myelodysplastic syndrome (MDS. The objectives were to determine the safety, pharmacokinetics, and hematologic improvement (HI rate. Phase 1-2a testing of ezatiostat for the treatment of MDS was conducted in a multidose-escalation, multicenter study. Phase 1 patients received ezatiostat at 5 dose levels (50, 100, 200, 400 and 600 mg/m2 intravenously (IV on days 1 to 5 of a 14-day cycle until MDS progression or unacceptable toxicity. In phase 2, ezatiostat was administered on 2 dose schedules: 600 mg/m2 IV on days 1 to 5 or days 1 to 3 of a 21-day treatment cycle. Results 54 patients with histologically confirmed MDS were enrolled. The most common adverse events were grade 1 or 2, respectively, chills (11%, 9%, back pain (15%, 2%, flushing (19%, 0%, nausea (15%, 0%, bone pain (6%, 6%, fatigue (0%, 13%, extremity pain (7%, 4%, dyspnea (9%, 4%, and diarrhea (7%, 4% related to acute infusional hypersensitivity reactions. The concentration of the primary active metabolites increased proportionate to ezatiostat dosage. Trilineage responses were observed in 4 of 16 patients (25% with trilineage cytopenia. Hematologic Improvement-Erythroid (HI-E was observed in 9 of 38 patients (24%, HI-Neutrophil in 11 of 26 patients (42% and HI-Platelet in 12 of 24 patients (50%. These responses were accompanied by improvement in clinical symptoms and reductions in transfusion requirements. Improvement in bone marrow maturation and cellularity was also observed. Conclusion Phase 2 studies of ezatiostat hydrochloride liposomes for injection in MDS are supported by the tolerability and HI responses observed. An oral formulation of ezatiostat hydrochloride tablets is also in phase 2 clinical development. Trial Registration Clinicaltrials.gov: NCT00035867

  8. Temperature profile data from XBT casts from the SEA-LAND ENTERPRISE and other vessels in the North/South Atlantic Oceans from 01 January 199 to 31 December 1999 (NODC Accession 0000684)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Temperature profiles were collected from XBT casts in the North/South Atlantic Oceans from the SEA-LAND ENTERPRISE and other vessels. Data were collected in support...

  9. Estudo comparativo da eficácia da drenagem linfática manual e mecânica no pós –operatório de dermolipectomia - doi:10.5020/18061230.2005.p199

    OpenAIRE

    Lúcia Maria Alves Soares; Sandra Mara Brasil Soares; Aline Kercia Alves Soares

    2012-01-01

    A abdominoplastia ou dermolipectomia é uma cirurgia que retira o excesso de pele e tecido adiposo do abdome de pacientes com ptose de pele ou flacidez em mulheres após múltiplas gestações. A drenagem linfática é uma técnica de massagem realizada com pressões suaves seguindo o trajeto do sistema linfático e atualmente é um dos recursos mais indicados no pós-operatório de cirurgias plásticas. O objetivo desse estudo foi comparar os efeitos da drenagem linfática manual (DLM) e da mecânica (DLME)...

  10. Estudo comparativo da eficácia da drenagem linfática manual e mecânica no pós –operatório de dermolipectomia - doi:10.5020/18061230.2005.p199

    Directory of Open Access Journals (Sweden)

    Lúcia Maria Alves Soares

    2012-01-01

    Full Text Available A abdominoplastia ou dermolipectomia é uma cirurgia que retira o excesso de pele e tecido adiposo do abdome de pacientes com ptose de pele ou flacidez em mulheres após múltiplas gestações. A drenagem linfática é uma técnica de massagem realizada com pressões suaves seguindo o trajeto do sistema linfático e atualmente é um dos recursos mais indicados no pós-operatório de cirurgias plásticas. O objetivo desse estudo foi comparar os efeitos da drenagem linfática manual (DLM e da mecânica (DLME no pós-operatório da dermolipectomia. Foi realizado um ensaio clínico randomizado com 14 mulheres de 35 a 45 anos, entre o oitavo e vigésimo dia de pós-operatório, avaliadas após um período de dez atendimentos por meio de medidas de perimetria, e melhoria dos sintomas. Também houve uma abordagem qualitativa, realizada mediante de um questionário, para analisar o grau de satisfação do paciente e do pesquisador em relação aos tratamentos empregados. Observou-se melhora quanto aos sintomas do pós-operatório e sua intensidade, nos dois grupos de tratamento. A DLM mostrou-se mais eficaz que a DLME na redução do edema especialmente na perimetria abdominal (p=0,01. A análise subjetiva do investigador e dos pacientes também revelou um maior índice de aceitação para a DLM, 57,1% e 85,7%, respectivamente. Conclui-se, portanto, que as duas técnicas foram eficazes e que a DLM mostrou-se mais eficiente do que a DLME no pós-operatório das pacientes submetidas a abdominoplastia-dermolipectomia

  11. Ground-water monitoring compliance projects for Hanford site facilities: Progress report for January 1 to March 31, 1988: Volume 9: Appendix C

    Energy Technology Data Exchange (ETDEWEB)

    1988-05-01

    The appendix is one of nine volumes, and presents data describing wells completed at the Hanford Site during the first quarter of calendar year 1988 (January through March). The data in this volume of Appendix C cover the following wells: 199-N-58; 199-N-59; 199-N-60; 199-N-61; 199-N-67. The data are presented in the following order: Well Completion Report/Title III Inspection List, As-Built Diagram, Logging Charts, and Drill Logs.

  12. Hurdles for a Broader Use of 211At and for the Synthesis of 211At-Labelled Radiopharmaceuticals at High Activities for Clinical Use

    International Nuclear Information System (INIS)

    One of the key impediments to the use of 211At is the very well known deleterious effect of high radiation fields caused by its alpha particles on the synthesis of 211At-labelled radiopharmaceuticals. This is problematic because radiolysis-mediated effects can produce diminishing efficiency of electrophilic astatination reactions due to increasing deposition of radiation dose with increasing activities and with the passage of the time. Astatine-211 has chemical properties that permit complex labelling strategies and a longer half-life than 213Bi that makes it more suitable when the targeting molecule does not gain immediate access to the tumour cells. The first clinical evaluation was published in 2001 [2] in patients with brain tumour. Although this study circumvents many of the challenges to entering clinical studies with 211At and many obstacles had to be surmounted before clinical studies could be initiated, several problems were encountered in maintaining efficient labelling with escalating radiation dose of α-particle even with fresh 211At elution [3]. Astatine-211 also has an additional hurdle to overcome before to its clinical application in labelled radiopharmaceuticals related with its production and distribution. Among the potential group of promising α- emitter it is the only one produced by cyclotrons, but due to the scarcity of cyclotrons equipped with 25−30 MeV α-particle beams, it will of necessity be utilized in distant locations from the site of production. It presents a major chemical challenge because the diminishing efficiency of electrophilic astatination reactions with the passage of the time is well known, a problem likely related to the radiolysis produced by the high LET (linear energy transfer) meaning that large amounts of energy are deposited in a highly localized manner. This problem has been most comprehensively investigated to understand and evaluate the role of the radiolysis effects of astatine alpha particles in the synthesis

  13. $\\beta$-delayed fission, laser spectroscopy and shape-coexistence studies with radioactive At beams

    CERN Multimedia

    We propose to study the $\\beta$-delayed fission, laser spectroscopy and radioactive decay of the newly available pure beams of neutron-deficient and neutron-rich astatine (Z=85) isotopes. The fission probability and the fission fragment distribution of the even-even isotopes $^{194,196}$Po following the $\\beta$-decay of the isotopes $^{194,196}$At will be studied with the Windmill setup. In-source laser spectroscopy will be performed on the entire astatine isotopic chain, using a combination of the Windmill setup, ISOLTRAP MR-ToF and ISOLDE Faraday. Radioactive decay data will be acquired at the Windmill setup throughout those studies and contribute to the global understanding of the phenomenon of shape coexistence in the neutron-deficient lead region.

  14. Production cross section of At radionuclides from $^{7}$Li+$^{\\textrm{nat}}$Pb and $^{9}$Be+$^{\\textrm{nat}}$Tl reactions

    CERN Document Server

    Maiti, Moumita

    2011-01-01

    Earlier we reported theoretical studies on the probable production of astatine radionuclides from $^{6,7}$Li and $^{9}$Be-induced reactions on natural lead and thalliun targets, respectively. For the first time, in this report, production of astatine radionuclides has been investigated experimentally with two heavy ion induced reactions: $^{9}$Be+$^{\\textrm{nat}}$Tl and $^{7}$Li+$^{\\textrm{nat}}$Pb. Formation cross sections of the evaporation residues, $^{207,208,209,210}$At, produced in (HI, xn) channel, have been measured by the stacked-foil technique followed by the off-line $\\gamma$-spectrometry at the low incident energies ($<$50 MeV). Measured excitation functions have been explained in terms of compound nuclear reaction mechanism using Weisskopf-Ewing and Hauser-Feshbach model. Absolute cross section values are lower than the respective theoretical predictions.

  15. Radiopharmaceutical Chemistry of Targeted Radiopharmaceutics. Synthesis of 211At-Labeled Radiopharmaceuticals at High Activities for Clinical Use

    International Nuclear Information System (INIS)

    Targeted α-particle radiotherapy is an appealing approach to cancer treatment because of the potential for delivering curative doses of radiation to tumor with minimal damage to normal tissue due to a range equivalent to only a few cell diameters. Compared with β-emitters they have significant advantages from a radiobiological perspective. The LET of 211At α-particles is more than 400 times higher than the β-particles emitted by 90Y, in addition the distance between ionizing events is almost the same as that between the two strands of DNA, yielding a high probability of creating non-repairable DNA damage. It gives the ability to kill cancer cells not compromised by hypoxia, dose rate effects or cell cycle position, enhancing their attractiveness for targeted radiotherapy. However, translation of the concept to the clinic has been slow, many obstacles had to be surmounted before clinical studies could be initiated, the first clinical evaluation of a 211At- labeled mAb was made in 2001. This study circumvents many of the challenges to entering clinical studies with 211At. But several problems were encountered in maintaining efficient labeling with escalating radiation dose of alpha-particle likely related to radiolysis. The impact of the radiolysis produced by the α-particle over the labeling chemistry is much higher in comparison with typical β-emitters due to a deposition of energy in the solvent in a highly localized manner two orders of magnitude per unit volume higher than 90Y or 131I. Due to these difficulties a comprehensive basic science study about the radiolytic effects of astatine alpha-particles over the synthesis of 211At-labeled radiopharmaceuticals was carried out. Its main goal was overcoming the problem of the synthesis of 211At-labeled radiopharmaceuticals at the high activities necessaries for therapy and also to extend the shelf life of astatine elutions. Briefly this study held several steps, the first one was to study the role of solvent

  16. Radiohalogenation of biomolecules. An experimental study on radiohalogen preparation, precursor synthesis, radiolabeling and biodistribution

    International Nuclear Information System (INIS)

    Radiohalogens are widely used in nuclear medicine, both as tool for diagnostic in vivo imaging, and in radionuclide therapy. This study deals with the use of radiohalogens; separation, precursor synthesis, labeling and biological behavior. The focus is on 211At and 124I, the former being a candidate for nuclide therapy and the latter potentially useful for diagnostic imaging and Auger-electron based radiotherapy. For astatine the separation, labeling and some biological behavior is described, and for iodine the latter two. Astatine was separated from an irradiated bismuth target by dry distillation. A novel cryotrap was developed for the isolation of astatine and subsequent synthesis of radiolabeled compounds. 5-[211At]astato-2'-deoxyuridine (AUdR) and N-succinimidyl-4-[211At]astatobenzoate (SAB) were synthesized in 95% respectively 90% radiochemical yields. The former is incorporated into DNA of proliferating cells and can therefore be used as an endoradiotherapeutic agent. The latter is a conjugate for the astatination of proteins. Human epidermal growth factor (hEGF) was tagged with astatine using three approaches: a) direct labeling of native hEGF, b) conjugation with SAB, and c) direct labeling of an hEGF - 7-(3-aminopropyl)-7,8-dicarba-nido-undecaborate(1-) conjugate. The overall labeling yields were 3.5% for direct labeling, 44% for SAB and 70% for the hEGF-nido-carborane conjugate. A new route to N-succinimidyl 3- and 4- [124I]iodobenzoate, two reagents for radioiodination of proteins is described affording 90% radiochemical yield. Three radioiodinated analogs of PK11195, 1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)isoquinoline-3-carboxyam ide, a peripheral-type benzodiazepine receptor antagonist, were synthesized. All three analogs were obtained in >90% radiochemical yield. Synthesis and application of 5-[124I]iodo-2'-deoxyuridine (IUdR) is presented. The closo-dodecaborate anion was evaluated as prosthetic group for radioiodination of macromolecules

  17. Cyclic voltammetry of ion and electron transfer across the water/ionic liquid interfaces

    Czech Academy of Sciences Publication Activity Database

    Langmaier, Jan; Samec, Zdeněk

    2013-01-01

    Roč. 59, č. 3 (2013), s. 199-199. ISSN 0034-6691 R&D Projects: GA ČR GAP206/11/0707 Institutional support: RVO:61388955 Keywords : cyclic voltammetry * electrochemistry Subject RIV: CG - Electrochemistry

  18. Grids of Agricultural Pesticide Use in the Conterminous United States, 1992

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This spatial dataset consists of 199 1-kilometer (km) resolution grids depicting estimated agricultural use of 199 pesticides in 1992 for the conterminous United...

  19. 211At production and recovery: first results of a new start

    International Nuclear Information System (INIS)

    Full text: 211At is the only alpha-emitting isotope which can be introduced into organic molecular structures by covalent chemical binding and possesses superior decay characteristics for therapeutic applications when compared with other alpha emitters. However, its production requires a minimum of 27,5 MeV alpha-particles, which are relatively rarely available. In view of the revival of alpha-therapy approaches a production of 211At has been set up at the MHH cyclotron (MC-35). The first aim of this project was to establish a target- and work-up-system for the reliable production of several ten MBq-quantities of 211At for animal- and cell-research. An aluminum-target-system holds a 1.3 cm diameter Bi-disc of 0.25 mm thickness. The disk is pressed onto a the aluminum backing with 2 tons/cm2. The front is covered with a 125 μm Al foil, cooled by a He jet which is separated from the vacuum by a 250 μm Ti foil. Starting with 27.5 MeV alpha-particles the resulting target-energy is 25 MeV. This relatively low energy is chosen to avoid any contamination with 210At and 210Po in the first experimental period. A beam current of 10 μA for 15 min (9 mCoulomb) yields 16.4 MBq 211At theoretically. Astatine is recovered from the target by a dry distillation technique, using various gases as a transport support-medium. The small all-quartz distillation-apparatus is heated up to 900 oC. Astatine is recovered in a small cool able vessel containing various trapping media. Distillation and trapping can be monitored with small radioactivity monitors. The whole system fits in a cubical 0.5 m glove box. Continuous monitoring of the distillation process allowed to optimize the heating rate, the transport support-medium and its flow-rate. Various trapping solutions were tested for their efficiency. Different beam dispersion in the target seems to influence the release and distillation of astatine from the molten bismuth-target. Two different release patterns were observed. The first

  20. Väheväärtusliku vallasasja süstemaatiline vargus KarS § 199 lõike 2 punkti 9 tähenduses. Tartu Ringkonnakohtu kriminaalkolleegiumi määrus kriminaalasjas 1-11-11324 (Riigikohtu kriminaalkolleegiumi otsuses asjas 3-1-1-87-07 väljendatud seisu

    Index Scriptorium Estoniae

    Randma, Paavo, 1977-

    2013-01-01

    Riigikohus leidis lahendis 3-1-1-87-07, et süstemaatilisuse jaatamiseks on vaja isiku poolt vähemalt kolme varguse toimepanemist. Ringkonnakohus asus lahendis 1-11-11324 seisukohale, et varguste toimepanemisest kui inimese elustiilist saab väheväärtuslike vallasasjade varastamise korral rääkida vaid juhul, kui need pannakse toime teatud kindla ajavahemiku vältel

  1. What is the empirical evidence of the Fisher Effect upon colombian economy during the period 199O-2000? ¿Cuál es la evidencia empírica del efecto Fisher en la economía colombiana, 1980-2000?

    Directory of Open Access Journals (Sweden)

    Sáenz Castro Jorge Enrique

    2001-12-01

    Full Text Available In this essay an analysis is carried out on the Fisher Effect
    upon the colombian economy during 1980-2000 taking the quarterly data of nominal interest rate and the inflation rateo Our results show that the nominal interest rate and the inflation rate have a unitary root, that they have a cointegral relation, this is a
    long-run relationship and that the variations in inflation upon
    the nominal interest rate are in the form of one to one. Thus,
    our results suggest that during the period analysed (1980-2000
    increments in inflation, on the long-run (20 years are entirely
    transmited to the nominal interest rate.
    En este trabajo se hace un análisis sobre el Efecto Fisher para
    el caso colombiano durante el periodo 1980-2000 a partir de la
    información trimestral de la tasa de interés nominal y de la tasa de inflación. Los resultados muestran que la tasa de interés nominal y la inflación poseen una raiz unitaria, guardan una relación de cointegración o relación de largo plazo y los cambios en la inflación sobre la tasa de interés nominal se presentan de forma uno a uno. De esta manera, los resultados sugieren que para el periodo 1980-2000 los incrementos en la inflación en el largo plazo (20 años se transmiten en forma completa sobre la tasa de interés nominal.

  2. Secondary School Teachers Don’t Have Time to Engage in the Most Important Aspects of Information Literacy Due to Curricular Pressures. A Review of: Williams, D. and C. Wavell. “Secondary School Teachers’ Conceptions of Student Information Literacy.” Journal of Librarianship and Information Science 39.4 (2007: 199-212.

    Directory of Open Access Journals (Sweden)

    Lotta Haglund

    2009-03-01

    Full Text Available Objective – To examine school teachers’ understanding of student information literacy and to look at the implications of the findings for developing information literacy in students.Design – Qualitative research design (group discussions; verbal and written reflections.Setting – Secondary schools in the United Kingdom.Subjects – Secondary school teachers with various subject specializations.Methods – The study initially involved 31 secondary school teachers; 24 from seven schools in Scotland and seven from two schools in England. Participant teachers were self-selecting. The study took a phenomenographic approach. Teachers’ perceptions of their students’ abilities and their experiences and perceptions of their role in developing these abilities were examined through data collection in three stages. During the first stage, group discussions established the teacher’s initial conceptions of information literacy. Participants also filled out an information task grid which, together with focus questions, ensured that discussions were kept in the realm of practice. During the second stage of data collection, teachers observed and reflected on their students’ use of information in classroom activities. This was accompanied by informal site visits during which 26 of the participants were introduced to information literacy frameworks and definitions in order to contextualize reflections and discussions. Field notes were used to record these informal meetings. Six participants provided written feedback on reflections. In the third and final stage, a summary of themes from the first two stages was sent to 23 of the participants prior to a second group discussion in order to try to stimulate further reflection. Discussions were audio recorded and transcribed. The transcribed data were analyzed for the dimensions of variation of teachers’ conceptions of student information literacy and key elements associated with each category of description were determined.Main Results – The data revealed six main conceptions of student information literacy among the teachers: “finding information,” “linguistic understanding,” “makingmeaning,” “skills,” “critical awareness ofsources,” and “independent learning.” There was no particular hierarchy in their conceptualization, although independent learning seemed to be the ultimate goal. Teachers’ conceptions in the beginning ofthe study focused on the ability to find information, whereas after a period of reflection and further discussions, a broader and more complex understanding of information literacy appeared. Table 1 simplifies some of the data from the table in the article and shows conceptualizations as well as some of the contextual elements. Teachers felt that they had at least some control over the development of student ability to find information, develop skills, and critically appraise sources, even though these were not the highest learning priority outcomes. However, in the three areas of ability which the teachers regarded as of high learning priority (linguistic understanding, making meaning and independent learning, they felt that they had little control over the development of these abilities in the students.Conclusion – The teachers’ conceptions of student information literacy overlapped with the ideas in existing models and frameworks for information literacy. However, some areas of information literacy were not addressed by the teachers, i.e., ethical issues in the use of information. In the categories of description of high learning priority, the main reason for the low sense of control by the teachers seemed to be curricular pressures – “our content is prescribed and time allocated doing each of these things is prescribed so we have got limited room for maneuvering” (206. The teachers tended to regard information literacy as process and skills-orientated, with little connection to learning. The authors note that other research also suggests that school librarians also have difficulty relating information finding to learning in specific subject disciplines. There is a clear gap here as the authors point out that “there is a danger that students will not understand the significance of learning with information for academic achievement or for independent learning” (209.The authors suggest that the way forward for further development of information literacy in the classroom and library is through dialogue and that “librarian-teacher collaborations needs to be founded on shared understanding of the complex inter-relationship between information and learning” (209.

  3. MR staging accuracy for endometrial cancer based on the new FIGO stage

    International Nuclear Information System (INIS)

    Background: Magnetic resonance imaging (MRI) has been frequently used to determine a preoperative treatment plan for gynecologic cancers. However, the MR accuracy for staging an endometrial cancer is not satisfactory based on the old FIGO staging system. Purpose: To evaluate MR accuracy for staging endometrial cancer using the new FIGO staging system. Material and Methods: Between January 2005 and May 2009, 199 women underwent surgery due to endometrial cancer. In each patient, an endometrial cancer was staged using MR findings based on the old FIGO staging system and then repeated according to the new FIGO staging system for comparison. Histopathologic findings were used as a standard of reference. Results: The accuracy of MRI in the staging of endometrial carcinoma stage I, II, III, and IV using the old FIGO staging system were 80% (159/199), 89% (178/199), 90% (179/199), and 99% (198/199), respectively, compared to 87% (174/199), 97% (193/199), 90% (179/199), and 99% (198/199), respectively, when using the new FIGO staging criteria. The overall MR accuracy of the old and new staging systems were 51% (101/199) and 81% (161/199), respectively. Conclusion: MRI has become a more useful tool in the preoperative staging of endometrial cancers using the new FIGO staging system compared to the old one with increased accuracy

  4. Inclusive measurement of (p,πsup(-)xn) double charge exchange reactions on bismuth from threshold to 800 MeV

    International Nuclear Information System (INIS)

    The energy dependence of the total angle-integrated cross section for the production of astatine isotopes from (p,πsup(-)xn) double charge exchange reactions on bismuth (sup(209)Bi) was measured from 120 to 800 MeV using activation and radiochemical techniques. Chemical yields were estimated by direct radioassaying of sup(211)At activity in thin (approximately 1 mg/cmsup(2)), irradiated bismuth targets. Calculations of the contributions of secondary (2-step) reactions to these measured astatine yields were performed, based partially upon the observed sup(211)At activity although even at the highest energies, the contribution to products lighter than sup(207)At was negligible. These data for products with as many as 7 neutrons removed from the doubly coherent product (sup(210)At) display nearby gaussian shapes for the mass distributions of the astatine residues with the maximum occurring for about sup(204)At. The most probable momentum transfer deduced from these distributions for the initial πsup(-) production step was 335 MeV/c. The observed excitation functions display a behaviour similar to that observed for the yield of sup(210)Po from a (p,πsup(O)) reaction on sup(209)Bi, but radically different from that observed for inclusive πsup(-) reactions on a heavy nucleus. These data are discussed in terms of recent theoretical approaches to negative pion production from bismuth. In addition, a simple, schematic model is developed to treat the rapidly decreasing percentage of the total inclusive πsup(-) emission which is observed for this double charge exchange reaction. This model reflects the capacity of a nucleus to a source of internal energetic protons

  5. EFFECT OF MECHANICAL PROPERTIES OF MARTENSITE AND LOADING RATE ON DUAL PHASE STEELS

    OpenAIRE

    BAYRAM, Ali

    1998-01-01

    In this study, steel sheet materials were used in order to obtain dual-phase steel. Specimens for this purpose have been annealed in ferrite + astatine regions at the temperatures of 740, 760, 800 and 820 °C. The specimens were annealed at the different temperatures with corresponding times 20, 40 and 60 minutes and quenched into water. As a result of this dual-phase steels at different ferrite + martensite ratio were produced. Sheet specimens were tested at the range of loading rates of 1...

  6. Human radiation studies: Remembering the early years: Oral history of Dr. Patricia Wallace Durbin, Ph.D., conducted November 11, 1994

    International Nuclear Information System (INIS)

    This report is a transcript of an interview of Dr. Patricia Wallace Durbin by representatives of the US DOE Office of Human Radiation Research. Dr. Durbin was selected for this interview because of her knowledge of the human plutonium injections and her recollections of key figures, especially Joseph Hamilton. After a brief biographical sketch Dr. Durbin discusses her loss of research funding from DOE, her recollections concerning research into strontium metabolism as part of Project Sunshine, her recollections relating to the rationale for studies of human metabolism of radionuclides, her remembrances of Dr. Hamilton's Astatine and Plutonium research, and her experiences in gathering archival records concerning these researches

  7. Hormesis and the Salk Polio Vaccine

    OpenAIRE

    Calabrese, Edward J.

    2011-01-01

    The production of the Salk vaccine polio virus by monkey kidney cells was generated using the synthetic tissue culture medium, Mixture 199. In this paper’s retrospective assessment of this process, it was discovered that Mixture 199 was modified by the addition of ethanol to optimize animal cell survival based on experimentation that revealed a hormetic-like biphasic response relationship. This hormesis-based optimization procedure was then applied to all uses of Mixture 199 and modifications...

  8. 32 CFR 220.8 - Reasonable charges.

    Science.gov (United States)

    2010-07-01

    ..., oral surgery, orthodontics, pediatric dentistry and endodontics) will be based on a full cost of the... CFR 199.14 to reimburse authorized providers. There are some exceptions to this general rule, as... CHAMPUS Diagnosis Related Group (DRG) payment system rates under 32 CFR 199.14(a)(1). Certain...

  9. 40 CFR 266.230 - What conditions must you meet for your LLMW to qualify for and maintain a storage and treatment...

    Science.gov (United States)

    2010-07-01

    ... solely to recordkeeping); (2) Store your LLMW in tanks or containers in compliance with chemical compatibility requirements of a tank or container in 40 CFR 264.177, or 264.199 or 40 CFR 265.177, or 265.199... standards found in 40 CFR 265.16(a)(3); (4) Conduct an inventory of your stored conditionally exempt LLMW...

  10. Samarbejdspolitikkens lange arm

    DEFF Research Database (Denmark)

    Christensen, Claus Bundgård

    2015-01-01

    Anmeldelse af Hans Sode-Madsen: De hvide busser 1941-45. Reddet fra Hitlers helvede. 316 sider. 199,95 kr. Lindhardt og Ringhof.......Anmeldelse af Hans Sode-Madsen: De hvide busser 1941-45. Reddet fra Hitlers helvede. 316 sider. 199,95 kr. Lindhardt og Ringhof....

  11. Radiopharmaceuticals for SPECT cancer detection

    Science.gov (United States)

    Chernov, V. I.; Medvedeva, A. A.; Zelchan, R. V.; Sinilkin, I. G.; Stasyuk, E. S.; Larionova, L. A.; Slonimskaya, E. M.; Choynzonov, E. L.

    2016-08-01

    The purpose of the study was to assess the efficacy of single photon emission computed tomography (SPECT) with 199Tl and 99mTc-MIBI in the detection of breast, laryngeal and hypopharyngeal cancers. A total of 220 patients were included into the study: 120 patients with breast lesions (100 patients with breast cancer and 20 patients with benign breast tumors) and 100 patients with laryngeal/hypopharyngeal diseases (80 patients with laryngeal/hypopharyngeal cancer and 20 patients with benign laryngeal/hypopharyngeal lesions). No abnormal 199Tl uptake was seen in all patients with benign breast and laryngeal lesions, indicating a 100% specificity of 199Tl SPECT. In the breast cancer patients, the increased 199Tl uptake in the breast was visualized in 94.8% patients, 99mTc-MIBI—in 93.4% patients. The increased 199Tl uptake in axillary lymph nodes was detected in 60% patients, and 99mTc-MIBI—in 93.1% patients. In patients with laryngeal/hypopharyngeal cancer, the sensitivity of SPECT with 199Tl and 99mTc-MIBI was 95%. The 199Tl SPECT sensitivity in identification of regional lymph node metastases in the patients with laryngeal/hypopharyngeal cancer was 75% and the 99mTc-MIBI SPECT sensitivity was 17%. The data obtained showed that SPECT with 199Tl and 99mTc-MIBI can be used as one of the additional imaging methods in detection of tumors.

  12. Geochemical constraints on the hydrothermal origin of ferromanganese encrustations from the Rodriguez Triple Junction, Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Nath, B.N.; Pluger, W.L.; Roelandts, I.

    stream_size 13 stream_content_type text/plain stream_name Geol_Soc_Spl_Pub_(London)_1997_119_199.pdf.txt stream_source_info Geol_Soc_Spl_Pub_(London)_1997_119_199.pdf.txt Content-Encoding ISO-8859-1 Content-Type text...

  13. Drug: D10331 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D10331 Drug Ozanezumab (USAN) C6446H10016N1712O2010S48 145104.076 145193.8836 (Heavy chain) QVQL ... L199, L'23-L'93, L'139-L'199) Peptide Treatment of ALS ... and multiple sclerosis anti Nogo-A protein [HSA:57 ...

  14. Drug: D02601 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D02601 Drug Phenothiazine (INN); Thiodiphenylamin; Nemazine [veterinary] (TN) C12H9...NS 199.0456 199.2716 D02601.gif veterinary medicine map07029 Phenothiazines CAS: 92-84-2 PubChem: 17396771 L

  15. 32 CFR 220.14 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., see 32 CFR part 199.) The term has the same meaning as set forth in the CHAMPUS regulation (32 CFR 199...)(1) of the Social Security Act (42 U.S.C. 1395ss) and 42 CFR part 403, subpart B. No-fault insurance... intensity and are provided in an ambulatory procedure unit (APU) of the facility of the Uniformed...

  16. Tumor markers in pancreatic cancer: a European Group on Tumor Markers (EGTM) status report.

    LENUS (Irish Health Repository)

    Duffy, M J

    2012-02-01

    Pancreatic ductal adenocarcinoma is one of the most difficult malignancies to diagnose and treat. The aim of this article is to review how tumor markers can aid the diagnosis and management of patients with this malignancy. The most widely used and best validated marker for pancreatic cancer is CA 19-9. Inadequate sensitivity and specificity limit the use of CA 19-9 in the early diagnosis of pancreatic cancer. In non-jaundiced patients, however, CA 19-9 may complement other diagnostic procedures. In patients with resectable pancreatic cancer, presurgical and postresection CA 19-9 levels correlate with overall survival. In advanced disease, elevated pretreatment levels of CA 19-9 are associated with adverse patient outcome and thus may be combined with other factors for risk stratification. Most, but not all, reports indicate that serial levels of CA 19-9 correlate with response to systemic therapy. Use of CA 19-9 kinetics in conjunction with imaging is therefore recommended in monitoring therapy. Although several potential serum and tissue markers for pancreatic cancer are currently undergoing evaluation, none are sufficiently validated for routine clinical use. CA 19-9 thus remains the serum pancreatic cancer marker against which new markers for this malignancy should be judged.

  17. EFFECT OF MECHANICAL PROPERTIES OF MARTENSITE AND LOADING RATE ON DUAL PHASE STEELS

    Directory of Open Access Journals (Sweden)

    Ali BAYRAM

    1998-03-01

    Full Text Available In this study, steel sheet materials were used in order to obtain dual-phase steel. Specimens for this purpose have been annealed in ferrite + astatine regions at the temperatures of 740, 760, 800 and 820 °C. The specimens were annealed at the different temperatures with corresponding times 20, 40 and 60 minutes and quenched into water. As a result of this dual-phase steels at different ferrite + martensite ratio were produced. Sheet specimens were tested at the range of loading rates of 10, 50 and 259 mm/min. Strength properties of dual-phase steels were investigated depending on annealing temperature, ratio of martensite and loading rate.

  18. A route for polonium 210 production from alpha-particle irradiated bismuth-209 target

    International Nuclear Information System (INIS)

    A method is proposed for production of polonium-210 via the 209Bi(α,3n)210 At nuclear reaction. Bombardment of a bismuth-209 target was performed with a 37 MeV alpha-particle beam that leads to the production of astatine-210 (T1/2 = 8.1 h), which decays to polonium-210. It is purified from the bismuth target matrix by employing liquid-liquid extraction using tributyl phosphate (TBP) in para-xylene from 7 M hydrochloric acid. Back extraction of polonium-210 was performed by 9 M nitric acid. This method allows to purify a tracer amount of Po-210 (2.6 x 10-13 mol) from macroscopic amount of Bi (2.8 x 10-2 mol).

  19. Effective bond orders from two-step spin–orbit coupling approaches: The I{sub 2}, At{sub 2}, IO{sup +}, and AtO{sup +} case studies

    Energy Technology Data Exchange (ETDEWEB)

    Maurice, Rémi, E-mail: remi.maurice@subatech.in2p3.fr; Montavon, Gilles [SUBATECH, CNRS UMR 6457, IN2P3/EMN Nantes/Université de Nantes, 4 rue Alfred Kastler, BP 20722, 44307 Nantes Cedex 3 (France); Réal, Florent; Gomes, André Severo Pereira; Vallet, Valérie [Laboratoire PhLAM, CNRS UMR 8523, Université de Lille, 59655 Villeneuve d’Ascq Cedex (France); Galland, Nicolas [CEISAM, UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3 (France)

    2015-03-07

    The nature of chemical bonds in heavy main-group diatomics is discussed from the viewpoint of effective bond orders, which are computed from spin–orbit wave functions resulting from spin–orbit configuration interaction calculations. The reliability of the relativistic correlated wave functions obtained in such two-step spin–orbit coupling frameworks is assessed by benchmark studies of the spectroscopic constants with respect to either experimental data, or state-of-the-art fully relativistic correlated calculations. The I{sub 2}, At{sub 2}, IO{sup +}, and AtO{sup +} species are considered, and differences and similarities between the astatine and iodine elements are highlighted. In particular, we demonstrate that spin–orbit coupling weakens the covalent character of the bond in At{sub 2} even more than electron correlation, making the consideration of spin–orbit coupling compulsory for discussing chemical bonding in heavy (6p) main group element systems.

  20. Effective bond orders from two-step spin–orbit coupling approaches: The I2, At2, IO+, and AtO+ case studies

    International Nuclear Information System (INIS)

    The nature of chemical bonds in heavy main-group diatomics is discussed from the viewpoint of effective bond orders, which are computed from spin–orbit wave functions resulting from spin–orbit configuration interaction calculations. The reliability of the relativistic correlated wave functions obtained in such two-step spin–orbit coupling frameworks is assessed by benchmark studies of the spectroscopic constants with respect to either experimental data, or state-of-the-art fully relativistic correlated calculations. The I2, At2, IO+, and AtO+ species are considered, and differences and similarities between the astatine and iodine elements are highlighted. In particular, we demonstrate that spin–orbit coupling weakens the covalent character of the bond in At2 even more than electron correlation, making the consideration of spin–orbit coupling compulsory for discussing chemical bonding in heavy (6p) main group element systems

  1. Different ways to improve the clinical effectiveness of radioimmunotherapy in solid tumors

    Directory of Open Access Journals (Sweden)

    Chatal Jean-Francois

    2009-09-01

    Full Text Available Radioimmunotherapy (RIT has been proven effective in the treatment of radiosensitive non-Hodgkin lymphoma but, for radioresistant solid tumors, new approaches are necessary to improve the clinical effectiveness. A real improvement has been the introduction of the pretargeting technology which appeared to be able to significantly increase tumor-to-normal organ uptake ratios.Another very promising approach consists in associating RIT with other treatment modalities. Finally the use of alpha particle-emitting radionuclides such as astatin-211 or bismuth-213 (alpha-RIT should allow to efficiently eradicate disseminated microscopic clusters of tumor cells or isolated tumor cells which fit well with the short path length of alpha particles.

  2. Sequential radioimmunotherapy with 177Lu- and 211At-labeled monoclonal antibody BR96 in a syngeneic rat colon carcinoma model

    DEFF Research Database (Denmark)

    Eriksson, Sophie E; Elgström, Erika; Bäck, Tom;

    2014-01-01

    UNLABELLED: Alpha-particle emitters, such as astatine-211 (211At), are generally considered suitable for the treatment of small cell clusters due to their short path length, while beta-particle emitters, for example, Lutetium-177 (177Lu), have a longer path length and are considered better for...... small, established tumors. A combination of such radionuclides may be successful in regimens of radioimmunotherapy. In this study, rats were treated by sequential administration of first a 177Lu-labeled antibody, followed by a 211At-labeled antibody 25 days later. METHODS: Rats bearing solid colon...... carcinoma tumors were treated with 400 MBq/kg body weight 177Lu-BR96. After 25 days, three groups of animals were given either 5 or 10 MBq/kg body weight of 211At-BR96 simultaneously with or without a blocking agent reducing halogen uptake in normal tissues. Control animals were not given any 211At-BR96...

  3. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    CERN Document Server

    Marsh, B A; Imai, N; Seliverstov, M D; Rothe, S; Sels, S; Capponi, L; Rossel, R E; Franchoo, S; Wendt, K; Focker, G J; Kalaninova, Z; Sjoedin, A M; Popescu, L; Nicol, T; Huyse, M; Radulov, D; Atanasov, D; Kesteloot, N; Borgmann, Ch; Cocolios, T E; Lecesne, N; Ghys, L; Pauwels, D; Rapisarda, E; Kreim, S; Liberati, V; Wolf, R N; Andel, B; Schweikhard, L; Lane, J; Derkx, X; Kudryavtsev, Yu; Zemlyanoy, S G; Fedosseev, V N; Lynch, K M; Rosenbusch, M; Van Duppen, P; Lunney, D; Manea, V; Barzakh, A E; Andreyev, A N; Truesdale, V; Flanagan, K T; Molkanov, P L; Koester, U; Van Beveren, C; Wienholtz, F; Goodacre, T Day; Antalic, S; Bastin, B; De Witte, H; Fink, D A; Fedorov, D V

    2013-01-01

    At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar fr...

  4. Development of 211At Chemistry for Labelling Biomolecules

    International Nuclear Information System (INIS)

    Our studies have demonstrated that high recovery of 211At can be obtained using a “wet chemistry” approach to isolation from the bismuth target. We have also demonstrated that direct labelling of antibody-B10 conjugates with 211At can be performed in high yields, and that the astatinated antibodies are stable to in vivo deastatination. While these results will allow us to enter clinical studies with 211At-labelled antibodies, the chemistry associated with 211At is not fully understood. Therefore, it is important that many more basic studies to be conducted with 211At, so the optimal labelling reagent for each type of disease-targeting agent becomes apparent

  5. Alpha particle induced DNA damage and repair in normal cultured thyrocytes of different proliferation status

    DEFF Research Database (Denmark)

    Lyckesvärd, Madeleine Nordén; Delle, Ulla; Kahu, Helena;

    2014-01-01

    Childhood exposure to ionizing radiation increases the risk of developing thyroid cancer later in life and this is suggested to be due to higher proliferation of the young thyroid. The interest of using high-LET alpha particles from Astatine-211 ((211)At), concentrated in the thyroid by the same...... levels of γH2AX decreased during the first 24h in both cycling and stationary cultures and complete repair was seen in all cultures but cycling cells exposed to (211)At. Compared to stationary cells alpha particles were more harmful for cycling cultures, an effect also seen at the pChk2 levels...... cultures at a modest level of damage, clearly demonstrating that cell cycle status influences the relative effectiveness of alpha particles....

  6. Groundwater monitoring plan for the 183-H Solar Evaporation Basins

    International Nuclear Information System (INIS)

    Groundwater monitoring at the 183-H Solar Evaporation Basins is regulated under Washington Administrative Code 173-303-645. Proposed in this plan is the first phase of a final-status, corrective action monitoring program for the site. The monitoring network consists of four existing wells: 199-H4-3, 199-H4-7, 199-H4-12A, and 199-H4-12C. Well 199-H4-12C is completed at the base of the unconfined aquifer; the other wells are screened at the water table. Wells 199-H4-7 and 199-H4-12A are groundwater extraction wells used in a pump-and-treat system. Groundwater samples will be collected from each well annually. Samples will be analyzed for the following: (1) constituents of concern (i.e., chromium, nitrate, technetium-99, and uranium) and fluoride; (2) additional constituents to aid data interpretation (e.g., alkalinity, anions, and metals); and (3) field parameters routinely acquired at the wellhead (e.g., pH, specific conductance, temperature, and turbidity). The objective of monitoring during operation of the pump-and-treat system is to determine whether concentrations of the contaminants of concern are decreasing

  7. A low-toxic site-directed mutant of Clostridium perfringens ε-toxin as a potential candidate vaccine against enterotoxemia.

    Science.gov (United States)

    Li, Qing; Xin, Wenwen; Gao, Shan; Kang, Lin; Wang, Jinglin

    2013-11-01

    Clostridium perfringens epsilon toxin (ETX), one of the most potent toxins known, is a potential biological weapon; therefore, the development of an effective vaccine is important for preventing intoxication or disease by ETX. In this study, genetically detoxified epsilon toxin mutants were developed as candidate vaccines. We used site-directed mutagenesis to mutate the essential amino acid residues (His106, Ser111 and Phe199). Six site-directed mutants of ETX (mETX (H106P) , mETX (S111H) , mETX (S111Y) , mETX (F199H) , mETX (F199E) , mETX (S111YF199E) ) were generated and then expressed in Escherichia coli. Both mETX (F199E) and mETX (H106P) with low or non-cytotoxicity that retained their immunogenicity were selected to immunize mice 3 times, and the mouse survival data were recorded after challenging with recombinant wild-type ETX. mETX (F199E) induces the same protection as mETX (H106P) , which was reported previously as a promising toxin mutant for vaccine, and both of them could protect immunized mice against a 100× LD₅₀ dose of active wild-type recombinant ETX. This work showed that mETX (F199E) is another promising candidate vaccine against enterotoxemia and other diseases caused by ETX. PMID:23835363

  8. A germline polymorphism of thymine DNA glycosylase induces genomic instability and cellular transformation.

    Directory of Open Access Journals (Sweden)

    Ashley Sjolund

    2014-11-01

    Full Text Available Thymine DNA glycosylase (TDG functions in base excision repair, a DNA repair pathway that acts in a lesion-specific manner to correct individual damaged or altered bases. TDG preferentially catalyzes the removal of thymine and uracil paired with guanine, and is also active on 5-fluorouracil (5-FU paired with adenine or guanine. The rs4135113 single nucleotide polymorphism (SNP of TDG is found in 10% of the global population. This coding SNP results in the alteration of Gly199 to Ser. Gly199 is part of a loop responsible for stabilizing the flipped abasic nucleotide in the active site pocket. Biochemical analyses indicate that G199S exhibits tighter binding to both its substrate and abasic product. The persistent accumulation of abasic sites in cells expressing G199S leads to the induction of double-strand breaks (DSBs. Cells expressing the G199S variant also activate a DNA damage response. When expressed in cells, G199S induces genomic instability and cellular transformation. Together, these results suggest that individuals harboring the G199S variant may have increased risk for developing cancer.

  9. Novel serum tumor marker,RCAS1,in pancreatic diseases

    Institute of Scientific and Technical Information of China (English)

    Koji Yamaguchi; Munechika Enjoji; Manabu Nakashima; Makoto Nakamuta; Takashi Watanabe; Masao Tanaka

    2005-01-01

    AIM: As tumor markers for pancreatic carcinoma,carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9 have been used, but the sensitivity and specificity are not enough for the diagnosis of pancreatic carcinoma.METHODS: A novel serum tumor marker, RCAS1, was compared with two conventional serum tumor markers,CEA (highly specific for pancreatic cancer) and CA 19-9 (highly sensitive for pancreatic cancer), in 48 patients with pancreatic exocrine tumors.RESULTS: When the diagnosis of benign or malignant conditions was examined by one tumor marker, the sensitivity of RCAS1 alone (55%) was higher than that of CEA alone (27%) and the specificity of RCAS1 alone (92%) was greater than that of CA19-9 alone (78%). When examined by a combination of two markers, the sensitivity of a combination of RCAS1 and CA19-9 (95%) was superior to those of CA19-9 alone (78%), RCAS1 alone (55%, P = 0.002),CEA alone (27%) (P<0.001), RCAS1 and CEA (59%) and CA19-9 and CEA (82%).CONCLUSION: These results suggest that the combination of RCASL and CA19-9 is highly sensitive for pancreatic carcinoma.

  10. An improved synthesis of an 125I and 211At labelled benzamide for melanoma imaging

    International Nuclear Information System (INIS)

    Recent studies have indicated that benzamides can exhibit affinity for malignant melanoma and may be exploited diagnostically in the treatment of this cancer. Radioiodinated N-(2-diethylaminoethyl)-3-[123I/131I]iodo-4-methoxybenzamide (*I-IMBA) is a benzamide with promising diagnostic properties. A new synthesis procedure was developed to obtain 125I-IMBA suitable for use in vivo. The assets of the procedure include the use of less toxic reagents and better reproducible results when radiolabelling the precursor. The procedure also facilitates the synthesis of the astatinated N-(2-diethylaminoethyl)-3-[211At]astatine-4-methoxybenzamide (211At-AMBA), a new benzamide with a therapeutic potential. The regiospecific no-carrier-added 125I- and 211At-labeling of the benzamide is performed by demetalation of an organotin precursor. Using tributylstannyl as a leaving group, the radiochemical yield obtained after 15 minutes of reaction was 70 %-90 % for both 125I-IMBA and 211At-AMBA. The labelling was performed in a solution of MeOH:AcOH with NCS as the oxidising agent. The organotin precursor N-(2-diethylaminoethyl)-3-(tri-n-butylstannyl)-4-methoxy-benzamide was synthesized from 3-bromo-4-methoxybenzoic acid, with n-BuLi (2 eq) and Bu3SnCl (1 eq) in THF, giving 3-(tri-n-butylstannyl)-4-methoxybenzoic acid. The amide function was introduced by converting the acid group into an active N-succinimidyl-ester, a good leaving group in the reaction with 2-(diethylamino)ethylamine. The overall yield of the organotin precursor was 65 %

  11. New developments of the in-source spectroscopy method at RILIS/ISOLDE

    Energy Technology Data Exchange (ETDEWEB)

    Marsh, B.A., E-mail: bruce.marsh@cern.ch [CERN, CH-1211 Geneva (Switzerland); Andel, B. [Comenius University, Bratislava (Slovakia); Andreyev, A.N. [University of York, Department of Physics, York YO10 5DD (United Kingdom); Antalic, S. [Comenius University, Bratislava (Slovakia); Atanasov, D. [Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Barzakh, A.E. [Petersburg Nuclear Physics Institute, NRC Kurchatov Institute, 188300 Gatchina (Russian Federation); Bastin, B. [Grand Accérateur National d’Ions Lourds (GANIL), Bd Henri Becquerel, F-14076 Caen (France); Borgmann, Ch. [Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Capponi, L. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Cocolios, T.E.; Day Goodacre, T. [CERN, CH-1211 Geneva (Switzerland); University of Manchester, Manchester (United Kingdom); Dehairs, M. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Derkx, X. [University of the West of Scotland, School of Engineering, Paisley PA1 2BE (United Kingdom); De Witte, H. [KU Leuven, Celestijnenlaan 200D, B-3001 Leuven (Belgium); Fedorov, D.V. [Petersburg Nuclear Physics Institute, NRC Kurchatov Institute, 188300 Gatchina (Russian Federation); Fedosseev, V.N.; Focker, G.J. [CERN, CH-1211 Geneva (Switzerland); Fink, D.A. [Ruprecht-Karls Universität, Seminarstr. 2, 69117 Heidelberg (Germany); CERN, CH-1211 Geneva (Switzerland); Flanagan, K.T. [University of Manchester, Manchester (United Kingdom); Franchoo, S. [CSNSM-IN2P3-CNRS, Université Paris-Sud, 91406 Orsay (France); and others

    2013-12-15

    Highlights: • Upgrade of the lasers, detectors and data acquisition for in-source resonance ionization spectroscopy at ISOLDE. • First use of the ISOLTRAP MR-ToF MS in combination with laser spectroscopy at ISOLDE. • Resonance ionization of astatine for the study of its nuclear structure. -- Abstract: At the CERN ISOLDE facility, long isotope chains of many elements are produced by proton-induced reactions in target materials such as uranium carbide. The Resonance Ionization Laser Ion Source (RILIS) is an efficient and selective means of ionizing the reaction products to produce an ion beam of a chosen isotope. Coupling the RILIS with modern ion detection techniques enables highly sensitive studies of nuclear properties (spins, electromagnetic moments and charge radii) along an isotope chain, provided that the isotope shifts and hyperfine structure splitting of the atomic transitions can be resolved. At ISOLDE the campaign to measure the systematics of isotopes in the lead region (Pb, Bi, Tl and Po) has been extended to include the gold and astatine isotope chains. Several developments were specifically required for the feasibility of the most recent measurements: new ionization schemes (Po, At); a remote controlled narrow line-width mode of operation for the RILIS Ti:sapphire laser (At, Au, Po); isobar free ionization using the Laser Ion Source Trap, LIST (Po); isobar selective particle identification using the multi-reflection time-of-flight mass separator (MR-ToF MS) of ISOLTRAP (Au, At). These are summarized as part of an overview of the current status of the in-source resonance ionization spectroscopy setup at ISOLDE.

  12. Diagnostic double guarded low-volume uterine lavage in mares

    DEFF Research Database (Denmark)

    Christoffersen, Mette; Brandis, Louise; Samuelsson, Julia; Bojesen, Anders Miki; Troedsson, M.H.T.; Petersen, M. R.

    2014-01-01

    endometrial biopsy and double guarded swab. Endometrial biopsies (n=199), swabs (n=199) and double guarded lavage samples (n=199) were obtained from 34 mares at six different time points in four estrous cycles, and were evaluated cytologically and bacteriologically. Endometrial biopsies from the first cycle...... guarded lavage technique was an accurate method for diagnosing mares with endometritis and the risk of false positive samples is considered to be minimal compared to other flushing techniques described. Key words: Endometritis, double guarded lavage, diagnostic test, E. coli, β-hemolytic streptococci...

  13. Vliv učitele a podíl školy na pohybovou aktivitu dětí vedoucí ke zdraví

    Czech Academy of Sciences Publication Activity Database

    Blahutková, M.; Řehulka, Evžen

    -, č. 11 (2002), s. 197-199. ISSN 1210-5481 Institutional research plan: CEZ:AV0Z7025918 Keywords : health oriented physical education * well-being system * psychomotoric Subject RIV: AN - Psychology

  14. Co se děje na konvergentních okrajích litosférických desek?

    Czech Academy of Sciences Publication Activity Database

    Vaněk, Jiří; Hanuš, Václav; Špičák, Aleš

    2004-01-01

    Roč. 54, č. 4 (2004), s. 196-199. ISSN 0009-0700 Institutional research plan: CEZ:AV0Z3012916 Keywords : convergent plate margins * subduction zones * active volcanoes Subject RIV: DC - Siesmology, Volcanology, Earth Structure

  15. Fission-fragment mass distribution and estimation of the cluster emission probability in the gamma+.SUP.232 Th and .SUP.181 Ta reactions

    Czech Academy of Sciences Publication Activity Database

    Karamian, S. A.; Adam, Jindřich; Belov, A. G.; Čaloun, Pavel; Norseev, Yu. V.; Stegailov, V. I.

    Singapore: World scientific, 1997 - (von Egidy, T.; Hartmann, H.; Habs, D.; Lobner, K.; Nifenecker, H.), s. 199-204 [International Workshop on Research with Fission Fragment s. Benediktbeurn (DE), 28.10.1996-30.10.1996

  16. Primary and Secondary Qualities

    Czech Academy of Sciences Publication Activity Database

    Hill, James

    New York: Continuum, 2010 - ( Savonius -Wroth, S.; Schuurman, P.; Walmsley, J.), s. 199-201 ISBN 9780826428110 Institutional research plan: CEZ:AV0Z90090514 Keywords : primary and secondary qualities * Locke * corpuscularianism Subject RIV: AA - Philosophy ; Religion

  17. Mezinárodní folklorní festival Strážnice

    Czech Academy of Sciences Publication Activity Database

    Stavělová, Daniela

    Praha : Divadelní ústav, 2001, s. 198-199. ISBN 80-7008-112-0 Institutional research plan: CEZ:AV0Z9058907 Keywords : festival, stage production, folklore Subject RIV: AC - Archeology, Anthropology, Ethnology

  18. Adult Bone Marrow Stem Cells and Biocompatible Polymers in SCI Repair

    Czech Academy of Sciences Publication Activity Database

    Hejčl, Aleš; Jendelová, Pavla; Syková, Eva

    New York : NOVA SCIENCE PUBLISHERS, 2010 - (Berhardt, L.), s. 199-222 ISBN 978-1-60876-863-9 Institutional support: RVO:68378041 Keywords : spinal cord injury * central nervous system * stromal cells Subject RIV: FH - Neurology

  19. Otto Gutfreund architekt(onik)

    Czech Academy of Sciences Publication Activity Database

    Lahoda, Vojtěch

    Praha : Artefactum, 2013 - (Petrasová, T.; Platovská, M.), s. 189-199 ISBN 978-80-86890-47-0 Institutional support: RVO:68378033 Keywords : Cubism * sculpture * architecture Subject RIV: AL - Art, Architecture, Cultural Heritage

  20. Kolmnurkkelmus, vahendlikult toimepandud vargus ja nõude omastamine. Riigikohtu kriminaalkolleegiumi otsus 3-1-1-85-11 / Kristina Domaškina

    Index Scriptorium Estoniae

    Domaškina, Kristina

    2012-01-01

    Riigikohtu lahendist 3-1-1-85-11, mis käsitleb varguse, kelmuse ja omastamise objektiivsete koosseisude temaatikat. Materiaalõiguslikest küsimustest karistusseadustiku paragrahvide 199, 209 ja 201 järgi

  1. Intermittent hemodialysis is superior to continuous veno-venous hemodialysis/hemodiafiltration to eliminate methanol and formate during treatment for methanol poisoning

    Czech Academy of Sciences Publication Activity Database

    Zakharov, S.; Pelclová, D.; Navrátil, Tomáš; Běláček, J.; Kurcová, I.; Komzák, O.; Šálek, T.; Latta, J.; Turek, R.; Boček, R.; Kučera, C.; Hubáček, J. A.; Fenclová, Z.; Petřík, V.; Čermák, M.; Hovda, K. E.

    2014-01-01

    Roč. 86, č. 1 (2014), s. 199-207. ISSN 0085-2538 Institutional support: RVO:61388955 Keywords : Intermittent hemodialysis * hemodiafiltration * methanol poisonings Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 8.563, year: 2014

  2. OPEC and the international oil market: can a cartel fuel the engine of economic development?

    Czech Academy of Sciences Publication Activity Database

    Noguera, Jose; Pecchenino, R. A.

    2007-01-01

    Roč. 25, č. 1 (2007), s. 187-199. ISSN 0167-7187 Institutional research plan: CEZ:MSM0021620846 Keywords : OPEC * International oil market * oil export Subject RIV: AH - Economics Impact factor: 0.464, year: 2007

  3. Novel serum tumor marker, RCAS1, in pancreatic diseases

    OpenAIRE

    Yamaguchi, Koji; Enjoji, Munechika; Nakashima, Manabu; Nakamuta, Makoto; Watanabe, Takashi; Tanaka, Masao

    2005-01-01

    AIM: As tumor markers for pancreatic carcinoma, carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9 have been used, but the sensitivity and specificity are not enough for the diagnosis of pancreatic carcinoma.

  4. Between a Rock and a Hard Place. Czech Trade Unions in Domestic and European Arenas

    Czech Academy of Sciences Publication Activity Database

    Mansfeldová, Zdenka

    Stuttgart: ibidem-Verlag, 2015 - (Landgraf, C.; Pleines, H.), s. 181-199 ISBN 978-3-8382-0734-6 Institutional support: RVO:68378025 Keywords : Trade unions * europeanization * Czech republic Subject RIV: AD - Politology ; Political Sciences

  5. Study of the Neutron-rich Isotope Ar-46 Through Intermediate Energy Coulomb Excitation

    Czech Academy of Sciences Publication Activity Database

    Calinescu, S.; Ceceres, L.; Grévy, S.; Sorlin, O.; Sohler, D.; Stanoiu, M.; Negoita, F.; Clement, E.; Astabatyan, R.; Borcea, C.; Borcea, R.; Bowry, M.; Catford, W.; Dombradi, Z.; Franchoo, S.; Garcia, R.; Gillibert, R.; Guerin, H.; Thomas, J. C.; Kuti, I.; Lukyanov, S.; Lepailleur, A.; Maslov, V.; Morfouace, P.; Mrázek, Jaromír; Niikura, M.; Perrot, L.; Podolyak, Z.; Petrone, C.; Peniozhkevich, Y.; Roger, T.; Rotaru, F.; Stefan, I.; Vajta, Zs.; Wilson, E.

    2014-01-01

    Roč. 45, č. 2 (2014), s. 199-204. ISSN 0587-4254 Institutional support: RVO:61389005 Keywords : GANIL * detector Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 0.850, year: 2014

  6. Characterization of heavy chain CDR3 diversity in the developing fetal piglet

    Czech Academy of Sciences Publication Activity Database

    Sun, J.; Butler, J. E.; Weber, P.; Šinkora, Marek; Ford, S. P.; Christenson, R. K.

    Uppsala: International Union of Immunological Societies, 2001. s. 199. [International Veterinary Immunology Symposium /6./. 15.07.2001-20.07.2001, Uppsala] Institutional research plan: CEZ:AV0Z5020903 Subject RIV: EC - Immunology

  7. AcEST: BP912908 [AcEST

    Lifescience Database Archive (English)

    Full Text Available -2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Web..., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (199

  8. Diagnosing Diabetes and Learning about Prediabetes

    Science.gov (United States)

    ... Size: A A A Listen En Español Diagnosing Diabetes and Learning About Prediabetes There are several ways ... mg/dl – 199 mg/dl Preventing Type 2 Diabetes You will not develop type 2 diabetes automatically ...

  9. Tooth decay - early childhood

    Science.gov (United States)

    ... Ribeiro NM, Ribeiro MA. Breastfeeding and early childhood caries: a critical review. J Pediatr (Rio J) . 2004;80:S199-S210. Sexton S, Natale R. Risks and benefits of pacifiers. Am Fam Physician . 2009; ...

  10. Sociální inteligence, sociální kompetence

    Czech Academy of Sciences Publication Activity Database

    Baumgartner, František; Orosová, O.; Výrost, J.

    Praha : Grada Publishing, 2008, s. 199-215 ISBN 978-80-247-1428-8 Institutional research plan: CEZ:AV0Z70250504 Keywords : social intelligence * social competence * emotional intelligence Subject RIV: AN - Psychology

  11. K narativnímu pojetí autobiografické paměti: výzkumné možnosti metody čáry života

    Czech Academy of Sciences Publication Activity Database

    Vlčková, Irena; Blatný, Marek

    Olomouc: Univerzita Palackého v Olomouci, 2005, s. 183-199. ISBN 80-244-1159-8 Institutional research plan: CEZ:AV0Z70250504 Keywords : Autobiographical memory * narrative psychology Subject RIV: AN - Psychology

  12. Ilmus Rein Neggo "Vastutuuli. I" / Hannes Oja

    Index Scriptorium Estoniae

    Oja, Hannes

    1999-01-01

    rets. rmt.: Vastutuuli. I : valimik EELK Los Angelese koguduse ajakirjas "Vastutuuli" (1950-1965) avaldatud kirjasõna / koostanud ja toimetanud Rein Neggo. Los Angeles : Eesti Evangeelse Luterliku Kiriku Los Angelese kogudus, 1999. 199 lk. : ill.

  13. The Way We Applauded: How popular culture stimulates collective memory of the socialist past in Czechoslovakia - the case of the television serial Vyprávěj and its viewers

    Czech Academy of Sciences Publication Activity Database

    Carpentier-Reifová, I.; Gillárová, K.; Hladík, Radim

    London: Routledge, 2011 - (Imre, A.; Havens, T.; Lustyik, K.), s. 199-221 ISBN 978-0-415-89248-3 Institutional support: RVO:67985955 Keywords : memory * nostalgia * trauma * post-socialism * television Subject RIV: AA - Philosophy ; Religion

  14. Temperature, salinity, and oxygen profiles from bottle casts in the North Atlantic Ocean by the Ukrainian Scientific Centre of the Ecology of Sea from 25 November 1983 to 15 February 1990 (NODC Accession 0000434)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Bottle and other data were collected from the North Atlantic Ocean from 25 November 1983 to 15 February 199. Data were collected by the Ukrainian Scientific Centre...

  15. Temperature, salinity, and nutrients data from CTD and bottle casts in the North Atlantic Ocean from 01 April 1969 to 31 August 1995 (NODC Accession 0000426)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — CTD, bottle, and other data were collected from the CHARLES DARWIN and other vessels in the Atlantic Ocean from 01 April 1969 to 31 August 199. CTD data include...

  16. Sv. Vojtěch a Levý Hradec

    Czech Academy of Sciences Publication Activity Database

    Tomková, Kateřina

    2014-01-01

    Roč. 2007, č. 3 (2014), s. 199-208. ISSN 1803-1374 Institutional support: RVO:67985912 Keywords : Early Middle Age * Bohemia * St. Adalbert * stronghold Subject RIV: AC - Archeology, Anthropology, Ethnology

  17. Drug: D09952 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ACE VTHQGLSSPV TKSFNRGEC (Disulfide bridge: 23-93; 139-199; H130-L219, Dimer) Peptide Treatment of Alzheim...er's disease monoclonal antibody beta-amyloid CAS: 1178862-65-1 PubChem: 124490631 ...

  18. Reference: 2SSEEDPROTBANAPA [PLACE

    Lifescience Database Archive (English)

    Full Text Available an overlapping E-box/ABRE motif abolished high transcription of the napA storage-protein promoter in transgenic Brassica napus seeds. Planta 199:515-519 (1996) PubMed: 8818291; ...

  19. AcEST: DK962128 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0013_A14 554 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_A14. 5' end seq ... SREDLIKDAILAVRETL 199 Query: 363 QDEELSVTNCSVAVLGIEEPF HVLDVKALQGFIDSLEEKKEAGEGDVTVEGEPVEGTAGP 542 Q E L + ... C+VA+LG++EPF H LD +A+Q ID+ E+ E EG EGE EG A Sbjct: 200 QGETLKSSL ... CTVAILGVDEPF HFLDQEAIQKVIDTFEKVPEEEEG----EGEAGEGEAEA 255 Query: ... SKEDLIKDAIMAIRETL 199 Query: 363 QDEELSVTNCSVAVLGIEEPF HVLDVKALQGFIDSL----EEKKEAGEGDVTVEGEPVEG 530 Q E L + ...

  20. Combining multiple serum biomarkers in tumor diagnosis: A clinical assessment

    OpenAIRE

    Li, Xin; LU, JUN; Ren, Hui; CHEN, TIANJUN; Gao, Lin; DI, LIGAI; SONG, ZHUCUI; Zhang, Ying; Yang, Tian; THAKUR, ASMITANANDA; Zhou, Shu-Feng; Yin, Yanhai; Chen, Mingwei

    2012-01-01

    The present study aimed to assess the diagnostic/prognostic value of various clinical tumor markers, including carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), cytokeratin 19 (CYFRA21-1), α-fetoprotein (AFP), carbohydrate antigen-125 (CA-125), carbohydrate antigen-19.9 (CA-19.9) and ferritin, individually or in combination. The electro-chemiluminescence immunization method was performed to detect the levels of seven tumor markers in 560 cancer patients and 103 healthy subjects f...

  1. Cytokeratin 19-fragments (CYFRA 21-1) as a novel serum biomarker for response and survival in patients with advanced pancreatic cancer

    OpenAIRE

    Boeck, S.; Wittwer, C; Heinemann, V; Haas, M.; Kern, C; Stieber, P; Nagel, D; Holdenrieder, S

    2013-01-01

    Background: CYFRA 21-1 serves as biomarker in several epithelial malignancies. However, its role in pancreatic cancer (PC) has not yet been investigated. Methods: Within a prospective single-centre study serial blood samples were collected from patients with confirmed advanced PC. Pre-treatment values and weekly measurements of CYFRA 21-1, carbohydrate antigen 19-9 (CA 19-9) and carcinoembryonic antigen (assessed by Elecsys 2010, Roche Diagnostics) during palliative first-line chemotherapy we...

  2. AcEST: DK956014 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ens... 201 4e-50 tr|Q6T8H1|Q6T8H1_9ROSI Citrate synthase OS=Citrus junos PE=2 SV=1 199 9e-50 tr|O24135|O2413...1_9ROSI Citrate synthase OS=Citrus junos PE=2 SV=1 Length = 464 Score = 199 bits (507), Expect = 9e-50 Ident

  3. AcEST: DK952347 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0013_N13 532 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_N13. 5' end seq ... SV=1 199 5e-51 sp|P00053|CYC_CANSA Cytochrome c OS=Cannabis ... sativa PE=1 SV=1 199 8e-51 sp|P00071|CYC_PASSA Cyt ...

  4. A note about the rate-and-state-dependent friction model in a thermodynamic framework of the Biot-type equation

    Czech Academy of Sciences Publication Activity Database

    Roubíček, Tomáš

    2014-01-01

    Roč. 199, č. 1 (2014), s. 286-295. ISSN 0956-540X R&D Projects: GA ČR GAP201/10/0357 Institutional support: RVO:61388998 Keywords : non-linear differential equations * heat flow * plasticity Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 2.724, year: 2013 http://gji.oxfordjournals.org/content/199/1/286.full.pdf?keytype=ref&ijkey=Bxq4QAJg1lMyhdk

  5. Tumor markers as a diagnostic key for hilar Cholangiocarcinoma

    Directory of Open Access Journals (Sweden)

    Juntermanns B

    2010-08-01

    Full Text Available Abstract Objective Hilar cholangiocarcinoma is the fourth most common gastrointestinal malignancy. CA19-9 and CEA are helpful devices in the management of gastrointestinal malignancies and belong to clinical routine in surgical oncology. But the validity of these parameters in terms of tumor extension and prognosis of bile duct malignancies still remains unclear. Methods From 1998 to 2008, we obtained preoperative CA19-9 and CEA serum levels in 136 patients with hilar cholangiocarcinoma. We correlated tumor stage, resectability rate and survival with preoperative CA 19-9 and CEA serum levels. Results CA19-9 (UICC I: 253 ± 561 U/ml; UICC II: 742 ± 1572 U/ml; UICC III: 906 ± 1708 U/ml; UICC IV: 1707 ± 3053 U/ml and CEA levels (UICC I: 2.9 ± 3.8 U/ml; UICC II: 4.6 ± 6.5 U/ml; UICC III: 18.1 ± 29.6 U/ml; UICC IV: 22.7 ± 53.9 U/ml increase significantly with rising tumor stage. Patients with pre operative serum levels of CA19-9 (> 1000 U/ml and CEA (> 14.4 ng/ml showed a significant poorer resectability rate and survival than patients with lower CA19-9 and CEA serum levels respectively. Conclusion CA19-9 and CEA serum levels are associated with the tumor stage. If preoperatively obtained CA19-9 and CEA serum levels are highly elevated patients have an even worse survival and the frequency of irresectability is significantly higher.

  6. Drug: D10058 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available mer's disease Monoclonal antibody beta-amyloid map07056 Agents for Alzheimer-type dementia CAS: 955085-14-0 PubChem: 135626778 ... ... H362-H420, H'22-H'96, H'139-H'195, H'215-L'219, H'256-H'316, H'362-H'420, L23-L93, L139-L199, L'23-L'93, L'139-L'199) Peptide Alzhei

  7. Renovação parcial do meio de cultura como perspectiva de aumentar a eficiência de um sistema de cocultoura de embriões sem fluxo contínuo de CO2 Partial replacement of the culture medium in order to enhance a embryos coculture system without continuous CO2 flow

    Directory of Open Access Journals (Sweden)

    Marcos Antônio Lemos Oliveira

    1996-12-01

    Full Text Available Substituindo-se parcialmente (30% os meios de cultura TC M 199/NaHCO3 (199/NaHCO3 e TC M 199/25mM HEPES (199/HEPES a intervalos de 24, 48 ou 72 horas, testou-se a possibilidade de incrementar a obtenção de blastocistos expandidos (BLE a partir de embriões Mus musculus no estádio de duas células incubados sem fluxo contínuo de CO2. Após distribuição aleatória dos embriões em tubos de ensaio contendo 2ml de meio de cultura e monocamada celular de oviduto bovino, os referidos tubos foram hermeticamente fechados e colocados em estufa bacteriológica a 37°C durante 96 horas. A renovação do meio de cultura em qualquer período não incrementou a porcentagem de embriões que alcançou o estádio de BLE, todavia, o número total de BLE obtido com o 199/HEPES (79,0% foi superior (P £ 0,01 ao verificado com o 199/NaHCO3 (65,0%.It has been evaluated, in a coculture system without continuous CO2 flow, the possibility to enhance the percentage of blastocists obtained from two cells mouse embryos by partial (30% replacement (each 24, 48 and 72 hours of intervals of two culture media (TCM 199/NaHCO3 and TCM 199/25 mM HEPES. The embryos, randomiy allocated in closed cultures tubes with 2 ml of medium and bovine oviduct monolayer, were maintained at 37°C during 96 hours. No diferences between spreaded blastocists were verifica when the media were replaced at ali intervals, however, the medium TCM 199/25mM HEPES was significantiy more effective (P £ 0.01 then TCM 199/NaHCO3.

  8. Effect of Fibroblast Growth Factor 2 (FGF2 and Insulin Transferrin Selenium (ITS on In Vitro Maturation, Fertilization and Embryo Development in Sheep

    Directory of Open Access Journals (Sweden)

    Sukanta Mondal

    2015-08-01

    Full Text Available The present study evaluated the effect of fibroblast growth factor 2 (FGF2 and insulin-transferrin-selenium (ITS to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i TCM-199 (Tissue Culture Medium-199; (ii TCM-199+10 ng/mL FGF2; (iii TCM-199+20 ng/mL FGF2; (iv TCM-199+30 ng/mL FGF2; (v TCM-199+10 ng/mL ITS; (vi TCM-199+20 ng/mL ITS; (vii TCM-199+30 ng/mL ITS and (viii TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL and gentamicin (50 µg/mL. The maturation rate was assessed based on the degree of expansion of cumulus cells and identifying first polar body extrusion into perivitelline space. The matured oocytes were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant medium and the cleavage rate was checked after 42-48 h post insemination and further cultured for 6-7 days. Maturation and cleavage rates were significantly higher (P<0.05 in the oocytes cultured in TCM-199 +10% FBS+FSH (10 μg/mL supplemented with both 20 ng/mL ITS and 20 ng/mL FGF2 as compared to the control. It was concluded that the supplementation of ITS and FGF2 in maturation medium was beneficial for improving maturation and cleavage rates of sheep oocytes. The addition of ITS and FGF2 in embryo culture medium did not improve the development of sheep embryos.

  9. The effect of IVM and IVC media on in vitro development of bovine embryos

    Directory of Open Access Journals (Sweden)

    E.T Mergawati

    2000-12-01

    Full Text Available The purpose of this study was to examine the effect of medium combination of IVM and IVC on the in vitro development of bovine embryos. The study involved 4 groups in a 2 (IVM medium x 2 (IVC medium factorial in a randomized block design. Each group was replicated for 5 times. The treatments were as follows: TCM-199/CR1aa (T1; TCM-199/SOF (T2; B- 199/CR1aa (T3 and B-199/SOF (T4. Oocytes were aspirated from ovaries collected at local abattoirs using aspiration medium of PBS supplemented with 3% FCS and 0.1% Penicillin and Streptomycin. The oocytes were matured in medium of TCM-199 or B-199 supplemented with 10% FCS, hormones: 10μg/ml FSH+ 10μg/ml hCG+ 1μg/ml Estradiol. Maturation was maintained at 37oC for 22 hours in 5% CO2 incubator with high humidity. A method of BRACKETT & Oliphant (BO was used to fertilize the matured oocytes. The fertilization was incubated for 7 hours in the 5% CO2 incubator. Two culture media of CR1aa or SOF/AA/BSA were used to develop the fertilized oocytes undergo to morula and blastocyst embryos. The findings showed that the proportion of oocytes cleaved and formation of blastocysts were affected significantly by a combination of IVM and IVC media (P<0.05. A combination of B-199/SOF (T4 resulted in a higher blastocyst rate (32% than others (T3= 29%; T2=T1= 23%. This study suggests that either SOF/AA/BSA or CR1aa has similar competence in development of bovine embryos in vitro.

  10. Cortisol influence on testicular testosterone secretion in domestic cat: An in vitro study Ação do cortisol sobre a secreção de testosterona em testículos de gato doméstico: um estudo in vitro

    Directory of Open Access Journals (Sweden)

    Gelson Genaro

    2010-10-01

    Full Text Available The aim of the present experiment was to investigate the effect of corticosteroids (exogen on in vitro testosterone secretion after stress by transportation (40 minutes. Feline testes (Felis silvestris catus were incubated in the following media: TCM 199; TCM 199 + hCG 10_7M; TCM 199 + hydrocortisone 10_7M, or TCM 199 + hCG + hydrocortisone. The animals (n=21 were allocated into three groups: (S that arrived at 3 h prior to surgery, (A that remained in the laboratory for 36 h before being submitted to surgical procedure, and (C that were also allowed to remain for 36 hours in the laboratory before the surgical procedure, but whose testes had been incubated with hydrocortisone prior to incubation in the referred media. The results showed that group S secreted higher levels of testosterone, regardless of the culture media. It is noteworthy that the suppressing action of hydrocortisone sodium succinate led to a reduction in the testosterone concentration, despite the presence of hCG.O objetivo deste trabalho foi investigar o efeito da hidrocortisona sobre a secreção de testosterona após cultivo in vitro dos testículos, em distintas situações de estresse (transporte de gatos domésticos (Felis silvestris catus. Testículos foram incubados nos seguintes meios de cultura: TCM 199; TCM 199 + hCG 10_7M; TCM 199 + hydrocortisona 10_7M e TCM 199 + hCG + hidrocortisona. Os animais (n=21 foram alocados em 3 sub-grupos: (S animal admitido 3 horas antes da orquiectomia, (A animais orquiectomizados após 36 horas de permanência no biotério e (C animais que permaneceram por 36 horas no biotério antes da cirurgia e que tiveram seus testículos pré-incubados em hidrocortisona. Os resultados demonstraram que o grupo S secretou maiores valores de testosterona em todas as condições estudadas. É válido mencionar que a supressão promovida pela hidrocortisona também promoveu redução na concentração de testosterona no meio TCM 199, a despeito da

  11. Preliminary results on the use of Ca 15-3 in malignant ovarian pathology

    International Nuclear Information System (INIS)

    Ca 15-3 was evaluated as a tumoral marker for ovarian carcinoma and compared to Ca 125, Ca 19-9 and TPA. Fourteen women with untreated malignant epitelial ovarian neoplasm and 52 women with various gynecological pathologies (16 benign ovarian tumors, 9 endometriosis, 16 malignant uterine tumors, 8 malignant uterine tumors and 3 vulvar carcinomas) were studied. All patients were sampled once before surgery. Patients with ovarian carcinoma were also sampled monthly for 18 months during and after chemiotherapy. Serum concentrations were assayed with an immunoradiometric assay using monoclonal antibodies (Ca 15-3, Ca 125 and Ca 19-9) or with a radioimmunoassay using polyclonal antibodies (TPA). Sensitivity and specificity were 42.9% and 92.3% for Ca 15-3, 71.4% and 94.2% for Ca 125, 28.6% and 92.3% for Ca 19-9, 71.4% and 80.8% for TPA, respectively. When Ca 15-3 was evaluated in association with Ca 125 and Ca 19-9 sensitivity and specificity were 71.4% and 88.5% for Ca 15-3 + Ca 125, and 64.3% and 88.5% for Ca 15-3 + Ca 19-9 respectively. Ca 125 gave the best accuracy and specificity. Ca 15-3 was positively correlated to Ca 125 (P<0.01) but because of its lower sensitivity cannot be used alone for monitoring ovarian malignant epithelial neoplasms

  12. Preclinical characterization of recombinant human tissue kallikrein-1 as a novel treatment for type 2 diabetes mellitus.

    Directory of Open Access Journals (Sweden)

    Tadeusz Kolodka

    Full Text Available Modulation of the kallikrein-kinin system (KKS has been shown to have beneficial effects on glucose homeostasis and several other physiological responses relevant to the progression of type 2 diabetes mellitus (T2D. The importance of bradykinin and its receptors in mediating these responses is well documented, but the role of tissue kallikrein-1, the protease that generates bradykinin in situ, is much less understood. We developed and tested DM199, recombinant human tissue kallikrein-1 protein (rhKLK-1, as a potential novel therapeutic for T2D. Hyperinsulinemic-euglycemic clamp studies suggest that DM199 increases whole body glucose disposal in non-diabetic rats. Single-dose administration of DM199 in obese db/db mice and ZDF rats, showed an acute, dose-dependent improvement in whole-body glucose utilization. Sub-acute dosing for a week in ZDF rats improved glucose utilization, with a concomitant rise in fasting insulin levels and HOMA1-%B scores. After cessation of sub-acute dosing, fasting blood glucose levels were significantly lower in ZDF rats during a drug wash-out period. Our studies show for the first time that DM199 administration results in acute anti-hyperglycemic effects in several preclinical models, and demonstrate the potential for further development of DM199 as a novel therapeutic for T2D.

  13. Time course of effects of chemotherapy and radiotherapy on a human pancreatic cancer cell line (SUIT-2) in vitro

    International Nuclear Information System (INIS)

    In order to investigate time course of effects of chemotherapy and radiotherapy on pancreatic cancer, we used the monolayer culture of a human pancreatic cancer cell line, SUIT-2 which produces CEA and CA19-9. Into the culture various kinds of anti-cancer drugs (MMC, ADR, FAR, 5FU, FT, CDDP, CPT-11) were administered for a period of 2 days, followed by irradiation with 60Co. Observation period was 20 days, during which we measured LDH, CEA and Ca19-9 in medium, DNA, protein, CEA and CA19-9 in cells in addition to observation of cells through microscope. Effects of anti-cancer drugs on pancreatic cancer cells were greater than those of irradiation when doses of anti-cancer drugs were enough. By using a lot of anti-cancer drugs, LDH value in medium increased at early time and decreased later. CEA and Ca19-9 values in medium decreased with the lapse of time, while they were increased later when small doses of the drugs had been administered. DNA, protein, CEA and Ca19-9 values in cells showed a decrease in accordance with increased doses of the drugs. Effects of FT and CPT-11 were marginal compared with the other drugs. (author)

  14. Effects of the cofactor binding sites on the activities of secondary alcohol dehydrogenase (SADH).

    Science.gov (United States)

    Wang, Tao; Chen, Xiangjun; Han, Jun; Ma, Sichun; Wang, Jianmei; Li, Xufeng; Zhang, Hui; Liu, Zhibin; Yang, Yi

    2016-07-01

    SADHs from Thermoanaerobacter ethanolicus are enzymes that, together with various cofactors, catalyze the reversible reduction of carbonyl compounds to their corresponding alcohols. To explore how cofactors bind to SADH, TeSADH was cloned in this study, and Ser(199) and Arg(200) were replaced by Tyr and Asp, respectively. Both sites were expected to be inside or adjacent to the cofactor-binding domain according to computational a prediction. Analysis of TeSADH activities revealed that the enzymatic efficiency (kcat/Km) of the S199Y mutant was noticeably enhanced using by NADH, NADPH as cofactors, and similar with that of wild-type using by NADP(+), NAD(+). Conversely, the activity of the R200D mutant significantly decreased with all cofactors. Furthermore, in yeast, the S199Y mutant substantially elevated the ethanol concentration compared with the wild type. Molecular dynamics simulation results indicated the H-bonding network between TeSADH and the cofactors was stronger for the S199Y mutant and the binding energy was simultaneously increased. Moreover, the fluorescence results indicated the S199Y mutant exhibited an increased preference for NAD(P)H, binding with NAD(P)H more compactly compared with wild type. PMID:27016086

  15. CLINICAL USE OF COMBINED DETECTION WITH TUMOR MARKERS FOR PANCREATIC CANCER

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective To explore the value of clinical use of combined detection with tumor markers for pancreatic cancer. Methods Tumor markers CA242,CA19-9 and CA50 in serum of 32 patinets with pancreatic cancer;26 patients with non-pancreatic digestive tract cancers and 24 patietns with benign pancreatic or biliary tract diseases were measured by immunoradiometric assay (IRMA). Results The levels of three markers in serum and positive rates of patients with pancreatic cancer were higher than those of other patients. The effect of measurement combining CA242 with CA19-9 was the best. The sensitivity ,specificity and accuracy of diagnosis for pancreatic cancer were 92.6%, 73.8% and 81.2% respectively. The levels of CA242 and CA19-9 were positively relative to burden of pancreatic cancer, and serum levels of these two markers of patients with resectable pancreatic cancer were lower than those with unresectable, but on difference was observed for CA50. Conclusion Combined detection of serum CA242 and CA19-9 could prove the effectual indicator for finding the patients with pancreatic cancer in high risk population or for resectable pancreatic cancer. Pre-operative measurement of serum levels of CA242 and CA19-9 is helpful to evaluate the burden of the tumors and possiblity of resect for pancreatic cancers.

  16. Investigation of the E2 and E3 matrix elements in 200Hg using inelastic scattering

    Directory of Open Access Journals (Sweden)

    Rand E. T.

    2014-03-01

    Full Text Available A nuclear structure campaign has been initiated to investigate the isotopes of mercury near A = 199. Currently 199Hg provides the most stringent limit on an atomic electric dipole moment (EDM. The observation of a permanent EDM would represent a clear signal of CP violation from new physics beyond the Standard Model. Theoretical calculations for 199Hg are very difficult and give varied predictions for the excited-state spectrum. Understanding the E2 and E3 strengths in the neighbouring even-even isotopes of mercury will make it possible to develop a nuclear structure model for the Schiff strength based on these matrix elements, and thereby constrain present model predictions of the contribution of octupole collectivity to the Schiff moment of the nucleus.

  17. Detection of EGFR mutations in plasma and biopsies from non-small cell lung cancer patients by allele-specific PCR assays

    DEFF Research Database (Denmark)

    Weber, Britta; Meldgaard, Peter; Hager, Henrik; Wu, Lin; Wei, Wen; Tsai, Julie; Khalil, Azza; Nexo, Ebba; Sorensen, Boe S

    2014-01-01

    mutations in exons 18-21 of the EGFR gene, employing the cobas(®) EGFR Tissue Test and cobas(®) EGFR Blood Test (in development, Roche Molecular Systems, Inc., CA, USA). RESULTS: Test results were obtained in all 199 (100%) plasma samples and 196/199 (98%) of the biopsies. EGFR-activating mutations were......BACKGROUND: Lung cancer patients with mutations in the epidermal growth factor receptor (EGFR) are primary candidates for EGFR-targeted therapy. Reliable analyses of such mutations have previously been possible only in tumour tissue. Here, we demonstrate that mutations can be detected in plasma...... samples with allele-specific PCR assays. METHODS: Pairs of the diagnostic biopsy and plasma obtained just prior to start of erlotinib treatment were collected from 199 patients with adenocarcinoma of non-small-cell lung cancer. DNA from both sample types was isolated and examined for the presence of...

  18. The atomic electric dipole moment induced by the nuclear electric dipole moment; the magnetic moment effect

    CERN Document Server

    Porsev, S G; Flambaum, V V

    2010-01-01

    We have considered a mechanism for inducing a time-reversal violating electric dipole moment (EDM) in atoms through the interaction of a nuclear EDM (d_N) with the hyperfine interaction, the "magnetic moment effect". We have derived the operator for this interaction and presented analytical formulas for the matrix elements between atomic states. Induced EDMs in the diamagnetic atoms 129Xe, 171Yb, 199Hg, 211Rn, and 225Ra have been calculated numerically. From the experimental limits on the atomic EDMs of 129Xe and 199Hg, we have placed the following constraints on the nuclear EDMs, |d_N(129Xe)|< 1.1 * 10^{-21} |e|cm and |d_N(199Hg)|< 2.8 * 10^{-24} |e|cm.

  19. Effect of Somatic Cell Types and Culture Medium on in vitro Maturation, Fertilization and Early Development Capability of Buffalo Oocytes

    Directory of Open Access Journals (Sweden)

    H. Jamil*, H. A. Samad, N. Rehman, Z. I. Qureshi and L. A. Lodhi

    2011-04-01

    Full Text Available This study was designed to evaluate the efficacy of different somatic cell types and media in supporting in vitro maturation (IVM, in vitro fertilization (IVF and early embryonic development competence of buffalo follicular oocytes. Cumulus oocyte complexes were collected for maturation from follicles (>6mm of buffalo ovaries collected at the local abattoir. Oocytes were co-cultured in tissue culture medium (TCM-199 with either granulosa cells, cumulus cells, or buffalo oviductal epithelial cells (BOEC @ 3x106 cells/ml or in TCM-199 without helper cells (control at 39°C and 5%CO2 in humidified air. Fresh semen was prepared in modified Ca++ free Tyrode medium. Fertilization was carried out in four types of media: i Tyrode lactate albumin pyruvate (TALP, ii TALP+BOEC, iii modified Ca++ free Tyrode and iv modified Ca++ free Tyrode+BOEC. Fertilized oocytes were cultured for early embryonic development in TCM-199 with and without BOEC. Higher maturation rates were observed in the granulosa (84.24% and cumulus cells (83.44% than BOEC co culture system (73.37%. Highest fertilization rate was obtained in modified Ca++ free Tyrode with BOEC co culture (70.42%, followed by modified Ca++ free Tyrode alone (63.77%, TALP with BOEC (36.92% and TALP alone (10.94%. Development of early embryos (8-cell stage improved in TCM-199 with BOEC co culture than TCM-199 alone. From the results of this study, it can be concluded that addition of somatic cells (granulosa cells, cumulus cells results in higher maturation rates of buffalo follicular oocytes than BOEC co culture system, while fertilization rate improved in modified Ca++ free Tyrode with and without BOEC. Addition of BOEC to TCM-199 improved the developmental capacity of early embryo.

  20. Diagnostic value of tissue polypeptide specific antigen in serum for patients with pancreatic carcinoma

    International Nuclear Information System (INIS)

    Objective: To compare the diagnostic value of tissue polypeptide specific antigen (TPS) in serum, with the carbohydrate antigens (CA) 19-9, CA50, CA125 and CA242 in pancreatic carcinoma. Methods: Serum TPS was measured with enzyme linked immunosorbent assay (ELISA). CA19-9 and CA125 were measured with chemiluminescent immunoassay. CAS0 and CA242 were measured with immunoradiometric assay in 33 patients with pancreatitis, 34 patients with pathologically proven pancreatic carcinoma, and 35 patients with non-pancreatic malignancies. Statistic analysis was carried out with SPSS 9.0 software. Results: Patients with pancreatic carcinoma had relatively higher levels of TPS [(386.5 ± 315. 1) U/L] and CA19-9 [(10 820.9 ± 389.7) kU/L] when compared with patients with pancreatitis [(86. 2 ± 28.1) U/L and (61.5 ± 24.7) kU/L, respectively; F = 936. 42, P < 0. 001 ; F = 2217. 09, P < 0. 001], with a sensitivity and a specificity of 70.6% (48/68) and 57.4% (39/68), respectively, for TPS, and 82.4% (28/34) and 77.9% (53/68), respectively, for CA19-9. Diagnostic performance was further improved when TPS was assayed in combination with CA19-9, CA50, CA125 and CA242. Conclusion: Serum TPS has an incremental value in complementing CA19-9 in the diagnosis of pancreatic carcinoma. (authors)

  1. The MobM relaxase domain of plasmid pMV158: thermal stability and activity upon Mn2+ and specific DNA binding

    OpenAIRE

    Lorenzo-Diaz, F.; Dostal, L.; Coll, M.; Schildbach, J. F.; Menendez, M.; Espinosa, M

    2011-01-01

    Protein MobM, the relaxase involved in conjugative transfer of the streptococcal plasmid pMV158, is the prototype of the MOB(V) superfamily of relaxases. To characterize the DNA-binding and nicking domain of MobM, a truncated version of the protein (MobMN199) encompassing its N-terminal region was designed and the protein was purified. MobMN199 was monomeric in contrast to the dimeric form of the full-length protein, but it kept its nicking activity on pMV158 DNA. The optimal relaxase activit...

  2. Domain Modeling: NP_071371.3 [SAHG[Archive

    Lifescience Database Archive (English)

    Full Text Available NP_071371.3 chr20 Solution structure of three tandem repeats of zf-C2H2 domains fro...m human Kruppel-like factor 5 p2ebta_ chr20/NP_071371.3/NP_071371.3_holo_107-199.pdb blast 117G,119K,126C,128K,139H,143H,151C,154C,167H,170I,171H,179C,181I,182C,195H,196L,199H _ZN 0 ...

  3. AcEST: BP911473 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000005_D04 513 Adiantum capillus-veneris mRNA. clone: YMU001_000005_D04. BP911473 - Show ... /69 (52%), Gaps = 6/69 (8%) Frame = -3 Query: 199 SEPF TQLLHSLYVYPRV------ASLSRKKNLFIRVELRRDDSDIRKPPLEAIYS ... 59 (54%), Gaps = 6/59 (10%) Frame = -3 Query: 199 SEPF TQLLHSLYVYPRV------ASLSRKKNLFIRVELRRDDSDIRKPPLEAIYS ... 31 HRGPFNGQTKWLTPDLKD--GKAARGFGGSS-GSNDFHALDFRNFTRSEPF TQLLHSLYV 161 P N +KW D K+ + FG S ++DF A DFR+ TR+EP ... 25 ---PQNSNSKWNFFDGKEIPRNGSNAFGYSDFNADDFQAFDFRSTTRNEPF LQLFHCLYV 481 Query: 160 YPRVASLSRKKNLFIRVELRRDDSDI ...

  4. 联合检测七种血清肿瘤标志物在肺癌诊断中的价值%Diagnostic value of joint detection of seven kinds of serum tumor markers in lung cancer

    Institute of Scientific and Technical Information of China (English)

    马芳芳; 王厚照; 刘青

    2013-01-01

    目的 探讨血清癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原199(CA199)、鳞状细胞癌抗原(SCC-Ag)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA211)、胃泌素释放肽前体(ProGRP)对肺癌的诊断价值.方法 采用化学发光法检测60例肺癌患者和52例健康者血清中CEA、CA125、CA199、SCC Ag、NSE、CYFRA211和ProGRP水平和阳性率.结果 肺癌组血清CEA、CA125、CA199、SCC-Ag、NSE、CYFRA211和ProGRP水平和阳性率与健康对照组相比,差异均有统计学意义(P<0.05);联合检测7种肿瘤标志物诊断肺癌的灵敏度明显高于单项检测.结论 上述7种血清肿瘤标志物对肺癌的辅助诊断有一定价值,其联合检测对肺癌的早期诊断具有重要意义.%Objective To investigate the diagnostic value of carcinoembryonic antigen (CEA),carbohydrate antigen 125 (CA125),carbohydrate antigen 199(CA199),squamous cell carcinoma antigen(SCC-Ag),neuron-specific enolase(NSE),cytokeratin 19 fragment(CYFRA211),gastrin releasing peptide precursor(ProGRP) in lung cancer.Methods The serum content and the positive rate of CEA,CA125,CA199,SCC-Ag,NSE,CYFRA211,ProGRP of serum tumor markers were measured by chemiluminoimmunoassay in 60 patients with lung carcinoma and 52 healthy suhjectseople.Results The levels and positive rates of CEA,CA125,CA199,SCC-Ag,NSE,CYFRA211,ProGRP of serum tumor markers in lung cancer group were higher than those of healthy controls(P<0.05),and combined detection of seven kinds of tumor makers had high sensitivity for lung cancer diagnosis.Conclusion The seven kinds of serum tumor markers are helpful for diagnosis of lung cancer.Joint detection is very significant for the early diagnosis of lung cancer.

  5. Dicty_cDB: Contig-U13783-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available th of database: 656,320,666 Effective search space: 52505653280 Effective search space used: 52505653280 Neighboring words thres... ... 87 5e-16 BC064686_1( BC064686 |pid:none) Xenopus laevis hypothetical protei... 87 5e-16 AM920689_3220( ...), Expect = e-104 Identities = 188/199 (94%), Positives = 188/199 (94%) Frame = +...KFVGLEVLGDGNEAVINDLET IGSLLHKEDYIHKYPYDWRXXX Frame C: *tiyqrqatliklkw*yqq*fgqlhhgqfqqikqfvsivkwiif*lnqlnlnniemkcl* fqkkd*nh*qnhliles...; 5,674,871 total letters Score E Sequences producing significant alignments: (bits) Value

  6. Effect of insulin-like growth factor-1 during in vitro oocyte maturation and in vitro culture of bovine embryos

    OpenAIRE

    Quetglas M.D.; Coelho L.A.; Garcia J.M.; Oliveira Filho E.B.; Esper C.R.

    2001-01-01

    The effects of insulin-like growth factor-I (IGF-I) on in vitro maturation (IVM) (experiment I) and on in vitro embryo development (experiment II) of bovine oocytes fertilized in vitro, were evaluated in terms of cleavage (CR), blastocyst (BR) and hatching (HR) rates. For IVM, immature cumulus-oocyte complexes were cultured in TCM-199 medium supplemented with Hepes, sodium bicarbonate, sodium pyruvate, additives, fetal calf serum (B-199 medium) and gonadotropins (14 U/ml PMSG and 7 U/ml hCG)....

  7. Effect of insulin-like growth factor-1 during in vitro oocyte maturation and in vitro culture of bovine embryos Efeito do fator de crescimento semelhante à insulina-1 durante a maturação in vitro dos oócitos e cultivo in vitro de embriões bovinos

    OpenAIRE

    M.D. Quetglas; L.A Coelho; Garcia, J M; E.B. Oliveira Filho; C.R. Esper

    2001-01-01

    The effects of insulin-like growth factor-I (IGF-I) on in vitro maturation (IVM) (experiment I) and on in vitro embryo development (experiment II) of bovine oocytes fertilized in vitro, were evaluated in terms of cleavage (CR), blastocyst (BR) and hatching (HR) rates. For IVM, immature cumulus-oocyte complexes were cultured in TCM-199 medium supplemented with Hepes, sodium bicarbonate, sodium pyruvate, additives, fetal calf serum (B-199 medium) and gonadotropins (14 U/ml PMSG and 7 U/ml hCG)....

  8. Overinhibition of Mitogen-Activated Protein Kinase Inducing Tau Hyperphosphorylation

    Institute of Scientific and Technical Information of China (English)

    LI Hong-lian; CHEN Juan; LIU Shi-jie; ZHANG Jia-yu; WANG Qun; WANG Jian-zhi

    2005-01-01

    To reveal the relationship between mitogen-activated protein kinase (MAPK) and tau phosphorylation, we used different concentration of PD98059, an inhibitor of MEK (MAPK kinase), to treat mice neuroblastma (N2a) cell line for 6 h. It showed that the activity of MAPK decreased in a dose-dependent manner. But Western blot and immunofluorescence revealed that just when the cells were treated with 16 μmol/L PD98059, tau was hyperphosphorylated at Ser396/404 and Ser199/202 sites. We obtained the conclusion that overinhibited MAPK induced tau hyperphosphorylation at Ser396/404 and Ser199/202 sites.

  9. Drug: D00177 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D00177 Drug Methylmethionine sulfonium chloride (JAN); L-Methylmethionine sulfonium... chloride; Cabagin U (TN) C6H14NO2S. Cl 199.0434 199.6989 D00177.gif Same as: C04078 Therapeutic category: 2...ns 23 Digestive organ agents 232 Peptic ulcer agents 2321 Methylmethionines D00177...ro-oesophageal reflux disease (GORD) A02BX04 Methiosulfonium chloride D00177 Methylmethionine sulfonium chlo...ride (JAN) CAS: 1115-84-0 PubChem: 7847245 LigandBox: D00177 NIKKAJI: J246.885I ATOM 11 1 C1c C 33.4422 -17.

  10. Role of blood tumor markers in predicting metastasis and local recurrence after curative resection of colon cancer

    OpenAIRE

    Peng, Yifan; Zhai, Zhiwei; Li, Zhongmin; Wang, Lin; Gu, Jin

    2015-01-01

    Aim: To investigate the prognostic value of carcinoembryonic antigen (CEA), CA199, CA724 and CA242 in peripheral blood and local draining venous blood in colon cancer patients after curative resection. Methods: 92 colon cancer patients who received curative resection were retrospectively analyzed. The CEA, CA199, CA724 and CA242 were detected in peripheral blood and local draining venous blood. Results: Metastasis or local recurrence was found in 29 (29/92, 31.5%) patients during follow-up pe...

  11. Drug: D04681 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D04681 Drug Lazabemide (USAN/INN) C8H10ClN3O 199.0512 199.6375 D04681.gif Antiparkinsonian monoamine oxid...(4129) Tyrosine metabolism hsa00360(4129) Phenylalanine metabolism hsa00380(4129)...nergic synapse map07216 Catecholamine transferase inhibitors Target-based classificatio...n of drugs [BR:br08310] Enzymes Oxidoreductases monoamine oxidase B (MAO-B) [HSA:... Tryptophan metabolism hsa00982(4129) Drug metabolism - cytochrome P450 hsa04726(4129) Serotonergic synapse hsa04728(4129) Dopami

  12. EST Table: FS900509 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS900509 E_FL_ftes_39E07_R_0 10/09/28 66 %/199 aa ref|XP_001600958.1| PREDICTED: si...0/09/10 65 %/209 aa gnl|Amel|GB30214-PA 10/09/10 66 %/199 aa gi|189237999|ref|XP_001812951.1| PREDICTED...A 10/08/29 low homology 10/09/10 62 %/208 aa AGAP005604-PA Protein|2L:17803628:17804284:1|gene:MED20_ANOGA 1

  13. New holographic scalar field models of dark energy in non-flat universe

    Energy Technology Data Exchange (ETDEWEB)

    Karami, K., E-mail: KKarami@uok.ac.i [Department of Physics, University of Kurdistan, Pasdaran St., Sanandaj (Iran, Islamic Republic of); Research Institute for Astronomy and Astrophysics of Maragha (RIAAM), Maragha (Iran, Islamic Republic of); Fehri, J. [Department of Physics, University of Kurdistan, Pasdaran St., Sanandaj (Iran, Islamic Republic of)

    2010-02-08

    Motivated by the work of Granda and Oliveros [L.N. Granda, A. Oliveros, Phys. Lett. B 671 (2009) 199], we generalize their work to the non-flat case. We study the correspondence between the quintessence, tachyon, K-essence and dilaton scalar field models with the new holographic dark energy model in the non-flat FRW universe. We reconstruct the potentials and the dynamics for these scalar field models, which describe accelerated expansion of the universe. In the limiting case of a flat universe, i.e. k=0, all results given in [L.N. Granda, A. Oliveros, Phys. Lett. B 671 (2009) 199] are obtained.

  14. AcEST: DK953163 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0017_A19 614 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_A19. 5' end seq ... rator 3 OS=Mus musculus GN=S... 56 2e-07 sp|Q12116|TMS 1_YEAST Membrane protein TMS 1 OS=Saccharomyces cere ... za sativa subs... 199 8e-50 tr|Q10IH0|Q10IH0_ORYSJ TMS ... membrane family protein, putative, ex... 199 8e-50 ...

  15. Dicty_cDB: Contig-U06681-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available a 109 Database: CSM Posted date: Jun 9, 2004 7:35 PM Number of letters in database: 5,674,871 Number of se... than 10.0: 113 length of query: 199 length of database: 5,674,871 effective HSP length...: 15 effective length of query: 184 effective length of database: 5,571,296 effective se... of successful extensions: 2534702 Number of sequences better than 10.0: 35 Length of query: 199 Length of database...sfully gapped: 0 Length of query: 66 Length of database: 1,040,966,779 Length adjustment: 38 Effective length of quer

  16. The biokinetics of alpha-particle emitting radiopharmaceuticals

    International Nuclear Information System (INIS)

    The past two decades have seen wide interest in the application of alpha-particle emitting radionuclides for targeted endoradiotherapy and a large number of compounds labeled with 211At (T1/2 7.21 h), 212Bi (T1/2 1 h) or 213Bi (T1/2 0.78 h) have been studied. Knowledge of the biokinetic behaviour of such agents is important both for their optimal clinical exploitation and for general radiological protection purposes. Animal studies of the distribution and retention of 211At compounds, including ionic astatide, substituted aromatic compounds and labelled monoclonal antibodies, have provided new information on the biochemistry of astatine. With respect the thyroid gland the uptake of the astatide ion has been shown to be very much lower than that of the iodide ion. Less information is available for 212Bi-labelled radiopharmaceuticals. The available data for both 211At and 212Bi radiopharmaceuticals are reviewed. Cautious generic biokinetic models for inorganic and simple organic compounds of 211At and 212Bi; for [211At]-, and [212Bi]-biphosphonates and for [211At]-, and [212Bi]-monoclonal antibodies, are proposed for use in general radiological protection when compound-specific data are not available. (orig.)

  17. The radiolabeled monoclonal antibodies in immunoscintigraphy and radioimmunotherapy: current state and perspectives

    International Nuclear Information System (INIS)

    The antibodies can be satisfactorily labelled with technitium-99 m or indium-111 for tumor immunoscintigraphy. The immunoscintigraphy is not useful for the primary tumor diagnosis. It can be useful for the diagnosis of the some cancer extension and for recurrent tumor visualization. The immunoscintigraphy is widely competed with Positron Emission Tomography (PET) which gives accurate results. In the future the immunoscintigraphy, in pre-therapeutic stage, contribute to the estimation of the dose delivered to the tumor and to normal organs for adopting or not a radioimmunotherapy. The antibodies can also be labeled with Iodine-131 for an application in radioimmunotherapy (RIT). The RIT is efficient in the non-Hodgkin's lymphoma treatment because of their great radiosensitivity. Until now the results have been very modest in solid tumor treatment but methodological and biotechnological progresses have to improve the efficiency especially for the small tumors. In the future iodine-131 which requires the confinement (very expensive) of patients will be substituted by yttrium-90 beta emitter, more energetic than iodine-131 and can be injected in walking case. In the long term, the alpha emitter radionuclides (astatine-211 or bismuth-213) can be used for hematologic cancer treatment. In conclusion the future of radiolabeled monoclonal antibodies is essentially therapeutic. The radioimmunotherapy associated to the chemotherapy give promising perspectives for the radiosensitive cancer treatment and in general small solid tumor treatment (F.M.)

  18. Biological toxicity of intracellular radionuclide decay. Part of a coordinated programme on radiation biology of Auger emitters and their therapeutic applications

    International Nuclear Information System (INIS)

    Internal radiotherapy should be performed with short-lived radionuclides which emit high LET radiation and short ranged radiation, and accumulated within cancers. Based on these considerations, several radionuclides (tritium, copper-64, gallium-67, iodine-123, iodine 125, iodine-131 and astatine-211) were chosen and their toxicity was assessed using cell division in mammalian cultured cells as a criterion. It was apparent that the toxic effects obtained with 125I greatly exceeded those observed in cells treated with any other radionuclides. The possible hypotheses to explain the excessive radiosensitivity of 125I were discussed in relation to microdosimetry calculation. It was also found that the division delay induced by radionuclide decay is primarily due to damage to the cell nucleus but not to the plasma membrane. The key problem remains the development of agents which can serve as carriers for radionuclide accumulation within tumors. Although several promising approaches (Synkavit, tamoxifen, iododeoxyuridine, antibodies, liposomes) were investigated, only 125I-labelled Synkavit would be desirable for clinical application

  19. The biokinetics of alpha-particle emitting radiopharmaceuticals

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, D.M. [School of Chemistry, Cardiff Univ., Cardiff (United Kingdom); Duffield, J.R. [Faculty of Applied Sciences, Univ. of the West of England, Bristol (United Kingdom)

    2005-07-01

    The past two decades have seen wide interest in the application of alpha-particle emitting radionuclides for targeted endoradiotherapy and a large number of compounds labeled with {sup 211}At (T{sup 1}/{sub 2} 7.21 h), {sup 212}Bi (T{sup 1}/{sub 2} 1 h) or {sup 213}Bi (T{sup 1}/{sub 2} 0.78 h) have been studied. Knowledge of the biokinetic behaviour of such agents is important both for their optimal clinical exploitation and for general radiological protection purposes. Animal studies of the distribution and retention of {sup 211}At compounds, including ionic astatide, substituted aromatic compounds and labelled monoclonal antibodies, have provided new information on the biochemistry of astatine. With respect the thyroid gland the uptake of the astatide ion has been shown to be very much lower than that of the iodide ion. Less information is available for {sup 212}Bi-labelled radiopharmaceuticals. The available data for both {sup 211}At and {sup 212}Bi radiopharmaceuticals are reviewed. Cautious generic biokinetic models for inorganic and simple organic compounds of {sup 211}At and {sup 212}Bi; for [{sup 211}At]-, and [{sup 212}Bi]-biphosphonates and for [{sup 211}At]-, and [{sup 212}Bi]-monoclonal antibodies, are proposed for use in general radiological protection when compound-specific data are not available. (orig.)

  20. Effect of cetuximab in combination with alpha-radioimmunotherapy in cultured squamous cell carcinomas

    International Nuclear Information System (INIS)

    Aim: The monoclonal antibody cetuximab, targeting the epidermal growth factor receptor (EGFR), is a promising molecular targeting agent to be used in combination with radiation for anticancer therapy. In this study, effects of cetuximab in combination with alpha-emitting radioimmunotherapy (RIT) in a panel of cultured human squamous cell carcinomas (SCCs) were assessed. Methods: SCC cell lines were characterized and treated with cetuximab in combination with anti-CD44v6 RIT using the astatinated chimeric monoclonal antibody U36 (211At-cMAb U36). Effects on 211At-cMAb U36 uptake, internalization and cell proliferation were then assessed in SCC cells. Results: Cetuximab in combination with 211At-cMAb U36 mediated increased growth inhibition compared to RIT or cetuximab alone in two cell lines. However, cetuximab also mediated radioprotective effects compared to RIT alone in two cell lines. The radioprotective effects occurred in the cell lines in which cetuximab clearly inhibited cell growth during radiation exposure. Cetuximab treatment also influenced 211At-cMAb-U36 uptake and internalization, suggesting interactions between CD44v6 and EGFR. Conclusions: Results from this study demonstrate the vast importance of further clarifying the mechanisms of cetuximab and radiation response, and the relationship between EGFR and suitable RIT targets. This is important not only in order to avoid potential radioprotective effects, but also in order to find and utilize potential synergistic effects from these combinations.

  1. Tumor immunotargeting using innovative radionuclides.

    Science.gov (United States)

    Kraeber-Bodéré, Françoise; Rousseau, Caroline; Bodet-Milin, Caroline; Mathieu, Cédric; Guérard, François; Frampas, Eric; Carlier, Thomas; Chouin, Nicolas; Haddad, Ferid; Chatal, Jean-François; Faivre-Chauvet, Alain; Chérel, Michel; Barbet, Jacques

    2015-01-01

    This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality. PMID:25679452

  2. Tumor Immunotargeting Using Innovative Radionuclides

    Directory of Open Access Journals (Sweden)

    Françoise Kraeber-Bodéré

    2015-02-01

    Full Text Available This paper reviews some aspects and recent developments in the use of antibodies to target radionuclides for tumor imaging and therapy. While radiolabeled antibodies have been considered for many years in this context, only a few have reached the level of routine clinical use. However, alternative radionuclides, with more appropriate physical properties, such as lutetium-177 or copper-67, as well as alpha-emitting radionuclides, including astatine-211, bismuth-213, actinium-225, and others are currently reviving hopes in cancer treatments, both in hematological diseases and solid tumors. At the same time, PET imaging, with short-lived radionuclides, such as gallium-68, fluorine-18 or copper-64, or long half-life ones, particularly iodine-124 and zirconium-89 now offers new perspectives in immuno-specific phenotype tumor imaging. New antibody analogues and pretargeting strategies have also considerably improved the performances of tumor immunotargeting and completely renewed the interest in these approaches for imaging and therapy by providing theranostics, companion diagnostics and news tools to make personalized medicine a reality.

  3. Proceedings of transuranium elements

    International Nuclear Information System (INIS)

    The identification of the first synthetic elements was established by chemical evidence. Conclusive proof of the synthesis of the first artificial element, technetium, was published in 1937 by Perrier and Segre. An essential aspect of their achievement was the prediction of the chemical properties of element 43, which had been missing from the periodic table and which was expected to have properties similar to those of manganese and rhenium. The discovery of other artificial elements, astatine and francium, was facilitated in 1939-1940 by the prediction of their chemical properties. A little more than 50 years ago, in the spring of 1940, Edwin McMillan and Philip Abelson synthesized element 93, neptunium, and confirmed its uniqueness by chemical means. On August 30, 1940, Glenn Seaborg, Arthur Wahl, and the late Joseph Kennedy began their neutron irradiations of uranium nitrate hexahydrate. A few months later they synthesized element 94, later named plutonium, by observing the alpha particles emitted from uranium oxide targets that had been bombarded with deuterons. Shortly thereafter they proved that is was the second transuranium element by establishing its unique oxidation-reduction behavior. The symposium honored the scientists and engineers whose vision and dedication led to the discovery of the transuranium elements and to the understanding of the influence of 5f electrons on their electronic structure and bonding. This volume represents a record of papers presented at the symposium

  4. Silver impregnated nanoparticles of titanium dioxide as carriers for {sup 211}At

    Energy Technology Data Exchange (ETDEWEB)

    Cedrowska, Edyta; Lyczko, Monika; Piotrowska, Agata; Bilewicz, Aleksander [Institute of Nuclear Chemistry and Technology, Warsaw (Poland); Stolarz, Anna; Trcinska, Agnieszka [Warsaw Univ. (Poland). Heavy Ion Lab.; Szkliniarz, Katarzyna [Silesia Univ. Katowice (Poland). Inst. of Physics; Was, Bogdan [Polish Academy of Science, Cracow (Poland). Inst. of Nuclear Physics

    2016-08-01

    The {sup 211}At radioisotope exhibits very attractive nuclear properties for application in radionuclide therapy. Unfortunately use of {sup 211}At is limited, because astatine as the heaviest halogen forms weak bond with carbon atoms in the biomolecules which makes {sup 211}At bioconjugates unstable in physiological conditions. In our work we propose a new solution for binding of {sup 211}At which consists of using nanoparticles of titanium dioxide modified with silver atoms as carriers for {sup 211}At. Ag{sup +} cations have been absorbed on the nanometer-sized TiO{sub 2} particles (15 and 32 nm) through ion exchange process and were reduced in Tollens' reaction. The obtained TiO{sub 2}-Ag nanoparticles were labeled with {sup 211}At. It was found that labeling yields were almost quantitative under reducing conditions, while under oxidizing conditions they dropped to about 80%. The labeled nanoparticles exhibited very high stability in physiological salt, PBS buffer, solutions of peptides (0.001 M cysteine, 0.001 M glutathione) and in human blood serum. To make TiO{sub 2}/Ag nanoparticles well dispersed in water and biocompatible their surface was modified with a silane coupling agent containing poly(ethyleneglycol) molecules. The developed functionalization approach will allow us to attach biomolecules to the TiO{sub 2}/Ag surface.

  5. Development and radiotherapeutic application of 211At-labeled radiopharmaceuticals. Progress report, March 1, 1981-February 28, 1982

    International Nuclear Information System (INIS)

    This project is concerned with developing the potential of alpha-emitting radionuclides as agents for radiotherapy. Alpha-emitters seem ideally suited for his application because their high linear energy transfer and short range permit the deposition of considerable energy in a very small volume of tissue. Unlike the beta particles of 131I which have a range of about 1 to 2 mm in tissue, 5 to 7 MeV alpha particles would traverse only a few cell diameters. Among the available alpha-emitters, 211At appears most promising for therapeutic applications because, (1) it has some chemical similarities to iodine, an element that can readily be incorporated into numerous proteins and peptides, (2) it has a half-life that is long enough to permit chemical manipulation yet short enough to minimize destruction of healthy cells due to degradation of the label over time, (3) it can be produced conveniently using a cyclotron, and (4) alpha emission is associated with 100% of its decays with no accompanying beta emission. In the past year the evaluation of an astatine-tellurium colloid as an agent for the destruction of malignant ascites has been completed. The therapeutic efficacy of 211At-tellurium colloid has been compared with that of several beta-emitting radiocolloids. Studies on the application of monoclonal antibodies as carriers for selective delineation and destruction of malignant cell populations have also been initiated

  6. Nuclear and chemical data for life sciences

    International Nuclear Information System (INIS)

    Use of reactor produced radionuclides is popular in life sciences. However, cyclotron production of proton rich radionuclides are being more focused in recent times. These radionuclides have already gained attention in various fields, including life sciences, provided they are obtained in pure form. This article is a representative brief of our contributions in generating nuclear data for the production of proton rich radionuclides of terbium, astatine, technetium, ruthenium, cadmium, niobium, zirconium, rhenium, etc., which may have application in clinical, biological, agriculture studies or in basic research. The chemical data required to separate the product isotopes from the corresponding target matrix have been presented along with a few propositions of radiopharmaceuticals. It also emphasizes on the development of simple empirical technique, based on the nuclear reaction model analysis, to generate reliable nuclear data for the estimation of yield and angular distribution of emitted neutrons and light charged particles from light as well as heavy ion induced reactions on thick stopping targets. These data bear utmost important in radiation dosimetry. (author)

  7. Studies of Stable Octupole Deformations in the Radium Region

    CERN Multimedia

    2002-01-01

    The purpose of the present project is to locate and identify states in the atomic nuclei possessing stable pearshaped octupole deformation. Such states, formally related to the structures known in molecular physics, manifest themselves as families of parity doublets in odd nuclei.\\\\ \\\\ The best possibilities for observing stable octupole deformations are offered in the Ra-region. Both theoretical calculations and experimental indications support such expectations. Such indications are the non-observation of two-phonon octupole vibrational states in the ISOLDE studies of the even-even radium nuclei, and the reversed sign of the decoupling factor of the ground state band in |2|2|5Ra observed in the single-neutron transfer reactions. In order to establish the predicted strong E1 and E3-transitions between the parity doublets in odd nuclei with stable octupole deformations it is proposed to study conversion electrons in odd-mass francium radium and radon isotopes following the @b-decay of francium and astatine. \\...

  8. Effect of cetuximab in combination with alpha-radioimmunotherapy in cultured squamous cell carcinomas

    Energy Technology Data Exchange (ETDEWEB)

    Nestor, Marika, E-mail: marika.nestor@bms.uu.s [Unit of Otolaryngology and Head and Neck Surgery, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Unit of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, S-751 85 Uppsala (Sweden); Sundstroem, Magnus [Unit of Molecular Pathology, Department of Genetics and Pathology, Uppsala University (Sweden); Anniko, Matti [Unit of Otolaryngology and Head and Neck Surgery, Department of Surgical Sciences, Uppsala University, S-751 85 Uppsala (Sweden); Tolmachev, Vladimir [Unit of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, S-751 85 Uppsala (Sweden)

    2011-01-15

    Aim: The monoclonal antibody cetuximab, targeting the epidermal growth factor receptor (EGFR), is a promising molecular targeting agent to be used in combination with radiation for anticancer therapy. In this study, effects of cetuximab in combination with alpha-emitting radioimmunotherapy (RIT) in a panel of cultured human squamous cell carcinomas (SCCs) were assessed. Methods: SCC cell lines were characterized and treated with cetuximab in combination with anti-CD44v6 RIT using the astatinated chimeric monoclonal antibody U36 ({sup 211}At-cMAb U36). Effects on {sup 211}At-cMAb U36 uptake, internalization and cell proliferation were then assessed in SCC cells. Results: Cetuximab in combination with {sup 211}At-cMAb U36 mediated increased growth inhibition compared to RIT or cetuximab alone in two cell lines. However, cetuximab also mediated radioprotective effects compared to RIT alone in two cell lines. The radioprotective effects occurred in the cell lines in which cetuximab clearly inhibited cell growth during radiation exposure. Cetuximab treatment also influenced {sup 211}At-cMAb-U36 uptake and internalization, suggesting interactions between CD44v6 and EGFR. Conclusions: Results from this study demonstrate the vast importance of further clarifying the mechanisms of cetuximab and radiation response, and the relationship between EGFR and suitable RIT targets. This is important not only in order to avoid potential radioprotective effects, but also in order to find and utilize potential synergistic effects from these combinations.

  9. Alpha particle emitters in medicine

    International Nuclear Information System (INIS)

    Radiation-induced cancer of bone, liver and lung has been a prominent harmful side-effect of medical applications of alpha emitters. In recent years, however, the potential use of antibodies labeled with alpha emitting radionuclides against cancer has seemed promising because alpha particles are highly effective in cell killing. High dose rates at high LET, effectiveness under hypoxic conditions, and minimal expectancy of repair are additional advantages of alpha emitters over antibodies labeled with beta emitting radionuclides for cancer therapy. Cyclotron-produced astatine-211 (211At) and natural bismuth-212 (212Bi) have been proposed and are under extensive study in the United States and Europe. Radium-223 (223Ra) also has favorable properties as a potential alpha emitting label, including a short-lived daughter chain with four alpha emissions. The radiation dosimetry of internal alpha emitters is complex due to nonuniformly distributed sources, short particle tracks, and high relative specific ionization. The variations in dose at the cellular level may be extreme. Alpha-particle radiation dosimetry, therefore, must involve analysis of statistical energy deposition probabilities for cellular level targets. It must also account fully for nonuniform distributions of sources in tissues, source-target geometries, and particle-track physics. 18 refs., 4 figs

  10. Preparation of 211At-labeled humanized anti-Tac using 211At produced in disposable internal and external bismuth targets

    International Nuclear Information System (INIS)

    These studies describe the production and purification of 211At as well as the procedure for labeling humanized anti-Tac, the antibody to the α-chain of the IL-2 receptor (IL-2Rα), which has been shown to be a useful target for immunotherapy. The optimized protocol combines the advantages of the two-stage dry distillation procedure with the astatination of trialkylstannyl substances as labeling compounds for proteins. The 211At was produced by bombarding either an external or a recently developed disposable internal bismuth target with α-particles from a Cyclotron Corporation CS-30 cyclotron. The 211At was found to contain less than 0.01% 210At. The production rate for the external target was 0.15 mCi ± 0.056 μA-1 h-1 (n = 9) (5.55 MBq μA-1 h-1). The production rate for the internal target was 0.44 ± 0.14 mCi μA-1 h-1 (n = 16) (16.28 MBq μA-1 h-1)

  11. 77 FR 58050 - Refuge-Specific Hunting and Sport Fishing Regulations

    Science.gov (United States)

    2012-09-19

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Fish and Wildlife Service 50 CFR Part 32 Refuge-Specific Hunting and Sport Fishing Regulations CFR Correction Sec. 32.29 0 In Title 50 of the Code of Federal Regulations, parts 18 to 199, revised as...

  12. 77 FR 58051 - Refuge-Specific Hunting and Sport Fishing Regulations

    Science.gov (United States)

    2012-09-19

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF THE INTERIOR Fish and Wildlife Service 50 CFR Part 32 Refuge-Specific Hunting and Sport Fishing Regulations CFR Correction 0 In Title 50 of the Code of Federal Regulations, Parts 18 to 199, revised as of October 1,...

  13. Verbální a vizuální. K využití analogií mezi slovesným a výtvarným výrazem v interpretaci literárních textů

    Czech Academy of Sciences Publication Activity Database

    Jedličková, Alice

    Praha : Pedagogická fakulta Univerzity Karlovy, 2005 - (Slavík, J.), s. 213-239 ISBN 80-7290-199-0 Institutional research plan: CEZ:AV0Z90560517 Keywords : interdisciplinarity * visuality Subject RIV: AJ - Letters, Mass-media, Audiovision

  14. Experimental investigation and thermodynamic description of the In-Sb-Sn ternary system

    Czech Academy of Sciences Publication Activity Database

    Manasijevič, D.; Vřešťál, J.; Minic, D.; Kroupa, Aleš; Zivkovic, D.; Zivkovic, Z.

    2008-01-01

    Roč. 450, 1-2 (2008), s. 193-199. ISSN 0925-8388 R&D Projects: GA MŠk(CZ) OC08053 Institutional research plan: CEZ:AV0Z20410507 Keywords : In-Sb-Sn system * phase diagram * CALPHAD Subject RIV: BJ - Thermodynamics Impact factor: 1.510, year: 2008

  15. Cluster PEACE observations of electrons during magnetospheric flux transfer events

    Czech Academy of Sciences Publication Activity Database

    Owen, C. J.; Fazakerley, A. N.; Carter, P. J.; Coates, A. J.; Krauklis, I. C.; Szita, S.; Taylor, M. G. G. T.; Trávníček, Pavel; Watson, G.; Wilson, R. J.; Balogh, A.; Dunlop, M. W.

    2001-01-01

    Roč. 19, - (2001), s. 1509-1522. ISSN 0992-7689 Grant ostatní: European Space Agency - Prodex(XE) IMF CS/SFe/571/2000 Institutional research plan: CEZ:AV0Z3042911 Keywords : magnetospheric physics * magnetopause * cusp and boundary layers * solar wind -magnetosphere interactions Subject RIV: BE - Theoretical Physics Impact factor: 1.199, year: 2001

  16. ISABELLE superconducting power distribution system

    International Nuclear Information System (INIS)

    Research during the period June 1980 through May 1981 is described. Areas studied include: radioactive decay of 199Po and 201Po, 202At, 189Tl energy levels from Pb decay, 192Pb → 192Tl → 192Hg, and Ba decays; high spin states; inelastic scattering and reactions of heavy ions from deformed nuclei; and nuclear structure theory

  17. Vliv koroze pod napětím na bezpečnost provozu plynovodu (2. část)

    Czech Academy of Sciences Publication Activity Database

    Gajdoš, Lubomír; Šperl, Martin

    2009-01-01

    Roč. 89, č. 9 (2009), s. 195-199. ISSN 0032-1761 R&D Projects: GA MPO(CZ) FT-TA5/076 Institutional research plan: CEZ:AV0Z20710524 Keywords : corrosion cracking * pipeline * fracture toughness Subject RIV: JL - Materials Fatigue, Friction Mechanics

  18. On equivalent roughness of mobile bed at high shear stress

    Czech Academy of Sciences Publication Activity Database

    Matoušek, Václav; Krupička, Jan

    2009-01-01

    Roč. 57, č. 3 (2009), s. 191-199. ISSN 0042-790X R&D Projects: GA ČR GA103/06/0428 Institutional research plan: CEZ:AV0Z20600510 Keywords : bed shear * experiment * hydraulic transport * sediment transport Subject RIV: BK - Fluid Dynamics Impact factor: 1.000, year: 2009

  19. Arthroderma benhamiae jako původce tinea capitis produnda a tinea corporis u dětských pacientů

    Czech Academy of Sciences Publication Activity Database

    Mallátová, N.; Janatová, H.; Kocourková, K.; Hubka, Vít; Dobiášová, S.; Širůček, P.; Nováková, J.; Šimečková, K.

    2014-01-01

    Roč. 89, č. 4 (2014), s. 199-204. ISSN 1803-6597 Grant ostatní: Karlova Universita(CZ) 1344214 Institutional support: RVO:61388971 Keywords : tinea corporis * tinea capitis * children * zoofilic dermatophytes Subject RIV: EE - Microbiology, Virology

  20. A GIS-based approach to spatio-temporal analysis of environmental pollution in urban areas: A case study of Prague's environment extended by LIDAR data

    Czech Academy of Sciences Publication Activity Database

    Matějíček, L.; Engst, P.; Jaňour, Zbyněk

    2006-01-01

    Roč. 199, č. 3 (2006), s. 261-277. ISSN 0304-3800 R&D Projects: GA ČR GA205/02/0898 Institutional research plan: CEZ:AV0Z20760514 Keywords : environmental pollution * ordinary kriging * GIS Subject RIV: DG - Athmosphere Sciences, Meteorology Impact factor: 1.888, year: 2006 www.elsevier.com/locate/ecolmodel