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Sample records for assisted enucleation method

  1. Study of the efficiency of chemically assisted enucleation method for handmade cloning in goat (Capra hircus).

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    Akshey, Y S; Malakar, D; De, A K; Jena, M K; Sahu, S; Dutta, R

    2011-08-01

    The present investigation was carried out to find an efficient chemically assisted procedure for enucleation of goat oocytes related to handmade cloning (HMC) technique. After 22-h in vitro maturation, oocytes were incubated with 0.5 μg/ml demecolcine for 2 h. Cumulus cells were removed by pipetting and vortexing in 0.5 mg/ml hyaluronidase, and zona pellucida were digested with pronase. Oocytes with extrusion cones were subjected to oriented bisection. One-third of the cytoplasm with the extrusion cone was removed with a micro blade. The remaining cytoplasts were used as recipients in HMC. Goat foetal fibroblasts were used as nuclear donors. The overall efficiency measured as the number of cytoplasts obtained per total number of oocytes used was significantly (p < 0.05) higher in chemically assisted handmade enucleation (CAHE) than oriented handmade enucleation without demecolcine (OHE) (80.02 ± 1.292% vs. 72.9 ± 1.00%, respectively, mean ± SEM). The reconstructed and activated embryos were cultured in embryo development medium (EDM) for 7 days. Fusion, cleavage and blastocyst development rate were 71.63 ± 1.95%, 92.94 ± 0.91% and 23.78 ± 3.33% (mean ± SEM), respectively which did not differ significantly from those achieved with random handmade enucleation and OHE. In conclusion, chemically assisted enucleation is a highly efficient and reliable enucleation method for goat HMC which eliminates the need of expensive equipment (inverted fluorescence microscope) and potentially harmful chromatin staining and ultraviolet (UV) irradiation for cytoplast selection. © 2010 Blackwell Verlag GmbH.

  2. Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattle

    DEFF Research Database (Denmark)

    Vajta, Gábor; Maddox-Hyttel, Poul; Skou, Christina T.

    2005-01-01

    The purpose of the present study was to find an efficient and reliable assisted procedure for enucleation related to the handmade cloning (HMC) technique. After in vitro maturation oocytes were incubated in 0.5 µgmL-¹ demecolcine for 2 h. Subsequently, zonae pellucidae were digested with pronase...

  3. Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryos

    DEFF Research Database (Denmark)

    Li, J; Villemoes, K; Zhang, Y

    2009-01-01

    The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41â€"42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade......%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems...

  4. Holmium laser assisted ′anatomical′ enucleation of adenoma of benign hyperplasia of prostate

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    Shivadeo S Bapat

    2006-01-01

    Full Text Available Aims: To present our technique of Holmium Laser assisted "ANATOMICAL" enucleation of the benign prostatic adenoma (HoLEP in 219 patients. Procedure is based on the principle of digital enucleation of the adenoma from its surgical capsule, but performed entirely by perurethral endoscopic technique assisted by Holmium Laser. Materials and Methods: From March 2001 to November 2004, 219 patients under went HoLEP. After the initial cuts from bladder neck to verumontanum at 5 and 7 o′clock position, capsule is identified. The beak of the resectoscope sheath was inserted in the plane between the capsule and the adenoma and the adenoma was physically pushed away towards the urethra from the capsule. Laser was used to coagulate the bleeders, to cut the mucosal attachments and tough stromal tissue. Procedure was repeated for median and two lateral lobes. There was minimal bleeding and fluid absorption. Complications were few. Results: In 206 cases successful enucleation of the adenoma was carried out. First 13 cases formed part of the learning curve and were completed by standard transurethral resection of prostate (TURP. IPSS score dropped from average of 23 to 8 and peak flow improved from 20. No patient had postoperative urinary incontinence or stricture. Conclusions: HoLEP is an effective alternative to TURP. Ultimate end results replicate the end results of open enucleation of BPH without its morbidity and have all the advantages of endoscopic surgery. It offers distinct advantages over standard TURP as the incidence of blood transfusion and fluid absorption are greatly minimized.

  5. Enucleation is enucleation is enucleation is enucleation.

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    Herrmann, Thomas R W

    2016-10-01

    The latest update of the EAU Guidelines on Management of Non-Neurogenic Male Lower Urinary Tract Symptoms, incl. Benign Prostatic Obstruction in 2016 announced a novel acronym for transurethral Endoscopic Enucleation of the Prostate (EEP). This was inspired by a meta-analysis on randomized controlled trials on Holmium Laser Enucleation of the Prostate (HolEP) and bipolar enucleation versus open simple prostatectomy. EEP codes for the common ground of both techniques: "anatomical enucleation." Although study quality with regard to the availability of long-term randomized controlled trials is at the moment mostly available for HoLEP, and bipolar enucleation, the existing data of all other enucleating techniques that have been demonstrated to perform anatomical enucleation as well should also been summarized under the same term. This editorial is a call for embracing this acronym of EEP for all anatomical enucleating techniques in order to serve for the transition from the age of TURP and open prostatectomy toward the age of EEP.

  6. The Role of Hemoclips Reinforcement in the Ligation-Assisted Endoscopic Enucleation for Small GISTs in Gastric Fundus

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    Ge Nan

    2014-01-01

    Full Text Available Background. Endoscopic ultrasonography- (EUS- assisted band ligation has been proven to be a safe and effective procedure for the treatment of small gastrointestinal stromal tumors (GISTs apart from the relatively high risk of the postligation perforation of the gastric fundus. The aim of this study is to investigate the efficacy of hemoclip reinforcement in treating small GISTs in the gastric fundus. Method. During a standard endoscopy, a transparent cap attached to the endoscopic tip was placed over the lesion to exert sustained maximal aspiration before a rubber band was released. Once a definite ligation was confirmed by EUS, the tumor was enucleated. Four to 6 hemoclips were placed on the folds around the ligation band to reduce the tension of the ligation site. Results. The small GISTs were resected completely in 192 patients. Two cases of delayed perforation were found 72 hours after the procedure and successfully treated with an ordinary conservative method. Conclusion. Hemoclip-reinforced endoscopic band ligation with systematic follow-up using EUS appears to be a simple and effective technique for the resection of small GISTs in the gastric fundus.

  7. On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control

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    Akihiko Ichikawa

    2013-06-01

    Full Text Available In this study, we developed a microfluidic chip with a magnetically driven microrobot for oocyte enucleation. A microfluidic system was specially designed for enucleation, and the microrobot actively controls the local flow-speed distribution in the microfluidic chip. The microrobot can adjust fluid resistances in a channel and can open or close the channel to control the flow distribution. Analytical modeling was conducted to control the fluid speed distribution using the microrobot, and the model was experimentally validated. The novelties of the developed microfluidic system are as follows: (1 the cutting speed improved significantly owing to the local fluid flow control; (2 the cutting volume of the oocyte can be adjusted so that the oocyte undergoes less damage; and (3 the nucleus can be removed properly using the combination of a microrobot and hydrodynamic forces. Using this device, we achieved a minimally invasive enucleation process. The average enucleation time was 2.5 s and the average removal volume ratio was 20%. The proposed new system has the advantages of better operation speed, greater cutting precision, and potential for repeatable enucleation.

  8. Comparing renal function preservation after laparoscopic radio frequency ablation assisted tumor enucleation and laparoscopic partial nephrectomy for clinical T1a renal tumor: using a 3D parenchyma measurement system.

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    Zhu, Liangsong; Wu, Guangyu; Huang, Jiwei; Wang, Jianfeng; Zhang, Ruiyun; Kong, Wen; Xue, Wei; Huang, Yiran; Chen, Yonghui; Zhang, Jin

    2017-05-01

    To compare the renal function preservation between laparoscopic radio frequency ablation assisted tumor enucleation and laparoscopic partial nephrectomy. Data were analyzed from 246 patients who underwent laparoscopic radio frequency ablation assisted tumor enucleation and laparoscopic partial nephrectomy for solitary cT1a renal cell carcinoma from January 2013 to July 2015. To reduce the intergroup difference, we used a 1:1 propensity matching analysis. The functional renal parenchyma volume preservation were measured preoperative and 12 months after surgery. The total renal function recovery and spilt GFR was compared. Multivariable logistic analysis was used for predictive factors for renal function decline. After 1:1 propensity matching, each group including 100 patients. Patients in the laparoscopic radio frequency ablation assisted tumor enucleation had a smaller decrease in estimate glomerular filtration rate at 1 day (-7.88 vs -20.01%, p radio frequency ablation assisted tumor enucleation technology has unique advantage and potential in preserving renal parenchyma without ischemia damage compared to conventional laparoscopic partial nephrectomy, and had a better outcome, thus we recommend this technique in selected T1a patients.

  9. Enucleation ratio efficacy might be a better predictor to assess learning curve of holmium laser enucleation of the prostate

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    Chang Wook Jeong

    2012-06-01

    Full Text Available PURPOSE: To appraise the evaluation methods for learning curve and to analyze the non-mentor-aided learning curve and early complications following the holmium laser enucleation of the prostate. MATERIALS AND METHODS:One-hundred and forty (n=140 consecutive patients who underwent HoLEP from July 2008 to July 2010 by a single surgeon (SJO were enrolled. Perioperative clinical variables, including enucleation time, morcellation time, enucleation ratio (enucleation weight/transitional zone volume, enucleation efficacy (enucleated weight/enucleation time, enucleation ratio efficacy (enucleation ratio/enucleation time, and early complication rate were analyzed. RESULTS: Mean prostate volume was 62.7 mL (range 21-162 and preoperative International Prostate Symptom Score (IPSS was 19.0 (4-35. Mean enucleation time and morcellation time were 49.9±23.8 (S.D. min and 11.0±9.7 min, respectively. Median duration of postoperative indwelling catheter was 1 (1-7 day and median hospital stay was 1 (1-6 day. There were a total of 31 surgery-related complications in 27 patients (19.3%, and all were manageable. There was an increasing trend of enucleation efficacy in the first 50 cases. However, enucleation efficacy was linearly correlated with the prostate size (correlation coefficients, R=0.701, p<0.001. But, enucleation ratio efficacy could eliminate the confounding effect of the prostate size (R=-0.101, p=0.233. The plateau of enucleation ratio efficacy was reached around the twenty-fifth case. CONCLUSIONS: Our results demonstrated that the operative learning curve plateau is reached after about 25 cases. We propose that a more appropriate parameter for estimating the operative learning curve is enucleation ratio efficacy, rather than enucleation efficacy.

  10. Video-thoracoscopic enucleation of esophageal leiomyoma

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    Luh Shi-Ping

    2012-03-01

    Full Text Available Abstract Background Leiomyoma is the most common benign tumor of the esophagus. Surgical enucleation is indicated in case of symptoms or an unclear diagnosis, and open thoracotomy has long been the standard approach for this procedure. However, enucleation through video assisted thoracoscopic surgery (VATS has been developed as a preferred approach for most lesions in recent years. Method Herein we report our twelve patients (seven men and five women, with median age of 42 years from 2001 to 2009, who underwent enucleation through VATS for esophageal leiomyomas, with a size from 1 to 8 cm in diameter (median: 5, and at different locations, from the thoracic outlet to near the diaphragmatic level of the thoracic esophagus. Intraoperative fiberoptic esophagoscopy was performed in two patients for localization by illumination. A right-sided approach was performed in eight cases (upper two thirds of esophagus and the left-sided in another four cases (lower third of esophagus. Result The median operative time was 95 minutes (70 to 230 minutes. Four of them required small utility incisions (4-6 cm for better exploration and manipulation. There were no major complications, such as death or empyema due to leaks from mucosal tears, and the presenting symptoms were improved during the follow-up period, from 12 to 98 months. Conclusion VATS can be considered as an initial approach for most patients with esophageal leiomyomas, even large in size, irregular in shape, or at unfavorable location. It is a safe, minimally invasive, and effective treatment. However, conversion to open thoracotomy should be required for the sake of clinical or technical concern.

  11. Oocytes Polar Body Detection for Automatic Enucleation

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    Di Chen

    2016-02-01

    Full Text Available Enucleation is a crucial step in cloning. In order to achieve automatic blind enucleation, we should detect the polar body of the oocyte automatically. The conventional polar body detection approaches have low success rate or low efficiency. We propose a polar body detection method based on machine learning in this paper. On one hand, the improved Histogram of Oriented Gradient (HOG algorithm is employed to extract features of polar body images, which will increase success rate. On the other hand, a position prediction method is put forward to narrow the search range of polar body, which will improve efficiency. Experiment results show that the success rate is 96% for various types of polar bodies. Furthermore, the method is applied to an enucleation experiment and improves the degree of automatic enucleation.

  12. Laser prostate enucleation techniques.

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    Lerner, Lori B; Rajender, Archana

    2015-10-01

    Laser treatment of benign prostatic hyperplasia (BPH) through enucleation techniques has become increasingly more utilized in the field of urology. Laser enucleation of the prostate (LEP) is a transurethral procedure that employs several different types of lasers to dissect the adenoma from the surgical capsule in a retrograde fashion. We review basic laser physics and current laser prostate enucleation techniques. Holmium-LEP (HoLEP), Thulium-LEP (ThuLEP), Greenlight-LEP (GreenLEP) and Diode-LEP (DiLEP) applications are discussed. We summarize the current literature with respect to functional outcomes and complications. Although each laser device used for prostate enucleation has the same goal of removal of the adenoma from the surgical capsule, each has unique characteristics (i.e. wavelength, absorption rates) that must be understood by the practicing surgeon. Mastery of one LEP technique does not necessarily translate into facile use of an alternative enucleation energy source and/or approach. The various LEP techniques have demonstrated similar, if not superior, postoperative results to transurethral resection of the prostate (TURP), the current gold standard in the treatment of BPH. This article outlines the current LEP techniques and should serve as a quick reference for the practicing urologist.

  13. Enucleated Weight/Enucleation Time, Is It Appropriate for Estimating Enucleation Skills for Holmium Laser Enucleation of the Prostate? A Consideration of Energy Consumption.

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    Kim, Khae Hawn; Kim, Kwang Taek; Oh, Jin Kyu; Chung, Kyung Jin; Yoon, Sang Jin; Jung, Han; Kim, Tae Beom

    2018-01-01

    To date, the parameters for evaluating enucleation efficiency have only considered enucleation time, although operators simultaneously consume both time and energy during holmium laser enucleation of the prostate. This study was undertaken to find a better way of assessing enucleation skills, considering both enucleation time and consumed energy. One hundred (n=100) consecutive patients who underwent holmium laser enucleation of the prostate from April 2012 to April 2014 by a single surgeon were enrolled. Ten groups of 10 consecutive cases were used to analyze the parameters of enucleation efficiency. The mean enucleation time, consumed energy, and enucleated weight were 41.3±19.2 minutes, 66.2±36.0 kJ, and 26.6±21.8 g, respectively. Concerning learning curves, like enucleation time-efficacy (=enucleated weight/enucleation time), enucleation energy-efficacy (=enucleated weight/consumed energy) also had an increasing tendency. Enucleation ratio efficacy (=enucleated weight/transitional zone volume/enucleation time) plateaued after 30 cases. However, enucleation time-energy-efficacy (=enucleated weight/enucleation time/consumed energy) continued to increase after 30 cases and plateaued at 61 to 70 cases. Furthermore, one-way analysis of variance showed that group means for enucleation time-energy-efficacy (F=3.560, p=0.001) were significantly different, but that those of enucleation ratio efficacy (F=1.931, p=0.057) were not. When both time and energy were considered, enucleation skills continued to improve even after 30 cases and plateaued at 61 to 70 cases. Therefore, we propose that enucleation time-energy-efficacy should be used as a more appropriate parameter than enucleation ratio efficacy for evaluating enucleation skills.

  14. [Transurethral enucleation prostate with plasmakinetic energy. A new technique of enucleation].

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    Ramírez Mendoza, Antonio; Ramírez Zambrana, Antonio; Ramírez Zambrana, María Cristina

    2013-03-01

    We present a new technique of transurethral prostatic enucleation. METHODS. We use an Olympus resectoscope with two electrodes; one has a button or "mushroom" shape to vaporize and enucleate, and the other one the shape of a conventional "cutting loop electrode"to resect. With the button-shaped electrode, we vaporize transversally at the level of the veru montanum, extending the dissection towards the lateroapical lobes, looking for the capsular plane. Once it is found, we ascend vaporizing up to the 3 and 9 in the clock following the capsular plane. Subsequently, we vaporize longitudinally the anterior commissure and keep going down to join the previously vaporizatized area. Then enucleation is achieved by pushing the adenoma with the button-shaped electrode, from distal to proximal, at the capsular plane level. Before the enucleated lobe falls inside the bladder, we can proceed, with the help of the conventional cutting loop electrode, to its resection, which is very fast and bloodless. If there is middle lobe, enucleation is initiated with it, performing two lateral grooves lengthways down to the veru montanum. RESULTS. We have carried out 45 procedures since October 2011, with excellent functional results. Prostate volumes range between 40-120 gr, with an operating time of 30-90 minutes. Hospital stay was 24-48 h. and the catheter is removed in 4-5 days. Blood transfusions were not needed in any case, and we have not recorded complications within this first year of follow-up. CONCLUSIONS. This technique makes possible the enucleation of the adenoma with a good haemostasis control. It also provides a good visualization of the capsular plane. Vaporization of the prostatic tissue is similar to the use of other energy forces. We don't need to use a "morcellator" to extract the enucleated prostatic tissue. It is an economical and easily reproducible procedure, with a short learning curve.

  15. A simplified approach for oocyte enucleation in mammalian cloning.

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    Iuso, Domenico; Czernik, Marta; Zacchini, Federica; Ptak, Grazyna; Loi, Pasqualino

    2013-12-01

    Despite its success in almost all farm and laboratory animals, somatic cell nuclear transfer (SCNT) is still a low-efficiency technique. In this investigation, we determined the impact of each enucleation step on oocyte viability (assessed by parthenogenetic activation): Hoechst (HO) staining, cytochalasin B, ultraviolet (UV) exposure, and demecolcine. Our data showed that of all the factors analyzed, UV exposure impaired oocyte development (cleavage, 59% for untreated oocytes vs. 8% UV exposed; blastocyst stage, 32% untreated vs. 0% UV exposed). A minor toxicity was detected following demecolcine treatment (cleavage, 62%; blastocyst stage, 13%). Next, we compared HO/UV (canonical) and demecolcine-assisted enucleation (DAE), with a straight removal of metaphase chromosomes without any chemical or physical aid (straight enucleation). DAE improved the preimplantation development of sheep cloned embryos compared to HO/UV enucleation (cleavage, 38% vs. 19%; blastocysts, 17% vs. 4%), yet straight enucleation resulted in the highest cleavage and blastocysts rates (61% and 30%, respectively). We concluded that: (1) UV exposure harms sheep oocyte and embryo development; (2) DAE may represent an alternative approach, especially for unskilled operators; and (3) straight enucleation remains, in our estimation, the most reliable and least harmful protocol for SCNT.

  16. The Use of Bipolar Transurethral Enucleation for the Treatment of Large-Sized Benign Prostatic Hyperplasia

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    Sevryukov F.A.; Nakagawa Ken

    2012-01-01

    The aim of the investigation is to study the clinical efficiency of the transurethral enucleation of large-sized prostate adenoma based on a comparative analysis with the results of open adenomectomy. Materials and Methods. The research is based on the treatment results of 122 patients treated using the transurethral enucleation method, and 122 patients with open adenomectomy. Conclusion. The advantage of an operative method of bipolar transurethral enucleation of prostate over adenom...

  17. [Monopolar transurethral enucleation of benign prostatic hyperplasia. Our initial experience].

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    Tsarichenko, D G; Simberdeev, R R; Glybochko, P V; Alyaev, Y G; Rapoport, L M; Sukhanov, R B; Sorokin, N I; Enikeev, D V; Davydov, D S

    2016-08-01

    The choice of surgical treatment in patients with BPH is one of the most discussed issues in urology. In recent years, the surgical treatment of prostates of medium and large sizes by means of enucleation has become increasingly popular. The emergence of special loops to perform bipolar and monopolar enucleation using standard equipment for TURP has opened up new possibilities for the treatment of patients with BPH-transurethral monopolar enucleation BPH. In the period from December 2014 to the current time 35 monopolar enucleations were performed in the clinic of urology Sechenov FMSMU. The mean age was 70,3+/-3,7 years; Prostate volume was 60,3+/-12,5 cm3; IPSS / Qol 24,6+/-3,3 / 5,1+/-1,1; Qsr 7.7+/-2.1 ml/s. We used resectoscope 26 Ch with constant irrigation, Hook-electrode, pusher-electrode, as well as a standard set of electrodes for mono- and bipolar surgery during the procedure. Enucleated adenomatous nodes were resected either by mono- or bipolar TURP and were laundered by Rene-Alexander syringe, or morcellated. Comparative analysis of different methods of surgical treatment of prostatic hyperplasia (laser and monopolar enucleation) shows the advantages of monopolar enucleation in patients with prostatic hyperplasia. Monopolar enucleation of prostate hyperplasia is a radical, as well as a safe and effective surgical method in the treatment of patients with BPH. Further observation of the operated patients will allow us to make a final conclusion about the place of this technique in the treatment of patients with BPH and adequacy of data.

  18. LAPAROSCOPIC PANCREATIC RESECTION. FROM ENUCLEATION TO PANCREATODUODENECTOMY. 11-YEAR EXPERIENCE

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    Marcel Autran Cesar MACHADO

    2013-09-01

    Full Text Available Context Our experience with laparoscopic pancreatic resection began in 2001. During initial experience, laparoscopy was reserved for selected cases. With increasing experience more complex laparoscopic procedures such as central pancreatectomy and pancreatoduodenectomies were performed. Objectives The aim of this paper is to review our personal experience with laparoscopic pancreatic resection over 11-year period. Methods All patients who underwent laparoscopic pancreatic resection from 2001 through 2012 were reviewed. Preoperative data included age, gender, and indication for surgery. Intraoperative variables included operative time, bleeding, blood transfusion. Diagnosis, tumor size, margin status were determined from final pathology reports. Results Since 2001, 96 patients underwent laparoscopic pancreatectomy. Median age was 55 years old. 60 patients were female and 36 male. Of these, 88 (91.6% were performed totally laparoscopic; 4 (4.2% needed hand-assistance, 1 robotic assistance. Three patients were converted. Four patients needed blood transfusion. Operative time varied according type of operation. Mortality was nil but morbidity was high, mainly due to pancreatic fistula (28.1%. Sixty-one patients underwent distal pancreatectomy, 18 underwent pancreatic enucleation, 7 pylorus-preserving pancreatoduodenectomies, 5 uncinate process resection, 3 central and 2 total pancreatectomies. Conclusions Laparoscopic resection of the pancreas is a reality. Pancreas sparing techniques, such as enucleation, resection of uncinate process and central pancreatectomy, should be used to avoid exocrine and/or endocrine insufficiency that could be detrimental to the patient's quality of life. Laparoscopic pancreatoduodenectomy is a safe operation but should be performed in specialized centers by highly skilled laparoscopic surgeons.

  19. Transpalpebral eye enucleation in the standing horse

    DEFF Research Database (Denmark)

    Christophersen, Mogens Teken

    Transpalpebral eye enucleation in the standing horse. The Nordic Equine Veterinary Conference, Proceedings, Copenhagen. Denmark. Nov. 2011.......Transpalpebral eye enucleation in the standing horse. The Nordic Equine Veterinary Conference, Proceedings, Copenhagen. Denmark. Nov. 2011....

  20. The role of intraoperative ultrasound in small renal mass robotic enucleation

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    Roberta Gunelli

    2016-12-01

    Full Text Available Introduction: As a result of the growing evidence on tumor radical resection in literature, simple enucleation has become one of the best techniques associated to robotic surgery in the treatment of renal neoplasia, as it guarantees minimal invasiveness and the maximum sparing of renal tissue, facilitating the use of reduced or zero ischemia techniques during resection. The use of a robotic ultrasound probe represents a useful tool to detect and define tumor location, especially in poorly exophytic small renal mass. Materials and methods: A total of 22 robotic enucleations were performed on < 3 cm renal neoplasias (PADUA score 18 Pz 6/7 e 4 Pz 8 using a 12-5 MHz robotic ultrasound probe (BK Drop-In 8826. Results: Once kidney had been isolated from the adipose capsule at the site of the neoplasia (2, the exact position of the lesion could be easily identified in all cases (22/22, even for mostly endophytic lesions, thanks to the insertion of the ultrasound probe through the assistant port. Images were produced and visualized by the surgeon using the TilePro feature of the DaVinci surgical system for producing a picture-in-picture image on the console screen. The margins of resection were then marked with cautery, thus allowing for speedy anatomical dissection. This reduced the time of ischemia to 8 min (6-13 and facilitated the enucleation technique when performed without clamping the renal peduncle (6/22. No complications due to the use of the ultrasound probe were observed. Conclusions: The use of an intraoperative robotic ultrasound probe has allowed for easier identification of small, mostly endophytic neoplasias, better anatomical approach, shorter ischemic time, reduced risk of pseudocapsule rupture during dissection, and easier enucleation in cases performed without clamping. It is noteworthy that the use of intraoperative ultrasound probe allows mental reconstruction of the tumor through an accurate 3D vision of the hidden field during

  1. Piezosurgery versus conventional surgery in radicular cyst enucleation.

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    Kocyigit, Ismail Doruk; Atil, Fethi; Alp, Yunus Emre; Tekin, Umut; Tuz, Hakan H

    2012-11-01

    This study compared the use of piezosurgery and conventional surgery in radicular cyst enucleation. The study was conducted with 29 patients who were radiologically and cytologically prediagnosed with radicular cysts in the jaw region. Nineteen patients were treated using piezosurgery, and 10 were treated using conventional surgical procedures. Surgical procedures were evaluated according to the following criteria: hemorrhage, soft-tissue damage, manipulation complexity, major perforation areas on the enucleated cyst tissue, and approximate operation duration. Patients were monitored postoperatively and evaluated for hemorrhaging at 24, 48, and 72 hours following surgery. Follow-up was conducted to check for recurrences and ranged from 5 to 24 months. No complications were observed in any of the 20 patients treated using piezosurgery, although the duration of surgery was longer than expected. Of the 10 patients treated using conventional methods, hemorrhaging that affected the operation occurred in 3 cases, perforation of the cyst epithelium and difficulties in enucleation occurred in 5 cases, postoperative hemorrhage occurred in 2 cases, and recurrence was observed in 2 cases. Piezosurgery may be considered effective in procedures such as enucleation that require sensitive manipulation, despite the increase in the length of the overall surgical procedure. Given the results of the present study and the current lack of information in the literature regarding postoperative pain, infection, and long-term success rates associated with the use of piezosurgery in cyst enucleation, further study in this area is recommended.

  2. Impact of the off-clamp endoscopic robot-assisted simple enucleation (ERASE) of clinical T1 renal tumors on the postoperative renal function: Results from a matched-pair comparison.

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    Mari, Andrea; Morselli, Simone; Sessa, Francesco; Campi, Riccardo; Di Maida, Fabrizio; Greco, Isabella; Siena, Giampaolo; Tuccio, Agostino; Vittori, Gianni; Serni, Sergio; Carini, Marco; Minervini, Andrea

    2018-02-13

    To evaluate the surgical and functional outcomes of a matched-paired series of on-clamp vs off-clamp endoscopic robot-assisted simple enucleation (ERASE) and standardized renorraphy in a tertiary referral institution, to search for predictors of functional drop after surgery and to investigate the influence of off-clamp technique in patients presenting these characteristics. A matched-pair comparison of 120 on-clamp vs 120 off-clamp over 491 patients treated with ERASE was performed. Perioperative and functional outcomes were compared between groups. Patients treated with on-clamp and off-clamp technique had comparable complication and positive surgical margin rate. The off-clamp group had a significantly lower eGFR drop compared to the on-clamp group at 3rd postoperative day (POD) (1% vs 7%, p = 0.0001) and at 30th POD (2.5% vs 9%, p = 0.01) from baseline. This difference lost its statistical significance at 6th month and at last follow-up (median 40 months). At multivariable analysis the Charlson comorbidity index (OR 2.06, p 15% eGFR drop from baseline to last follow-up. In a subanalysis over 64 comorbid patients, those patients who underwent off-clamp ERASE had a significantly lower eGFR drop compared to the comorbid counterpart during the whole follow-up. The off-clamp ERASE is a safe surgical technique with a significantly lower renal function drop compared to on-clamp ERASE in the early perioperative time. Patients with comorbidity might represent a subgroup of patients having a functional benefit after off-clamp RAPN even in the long-term period. Copyright © 2018 Elsevier Ltd, BASO ~ The Association for Cancer Surgery, and the European Society of Surgical Oncology. All rights reserved.

  3. Preservation of enucleated porcine eyes for use in a wet laboratory

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    Nibourg, Lisanne M.; Koopmans, Steven A.

    PURPOSE: To design a method to preserve enucleated porcine eyes for use in a wet laboratory. SETTING: Laboratory of Experimental Ophthalmology, University Medical Center Groningen, the Netherlands. DESIGN: Experimental study. METHODS: Porcine eyes were preserved using 15 methods including salt

  4. Evolution and success of holmium laser enucleation of the prostate

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    Krambeck, Amy E.

    2010-01-01

    Aims: The purpose of this article is to review the development of instruments, current technique, and expected outcomes for holmium laser enucleation of the prostate (HoLEP). Materials and Methods: A review of published, peer-reviewed articles focusing on HoLEP was performed using the MEDLINE database. Results: Historically, the gold-standard management for symptomatic obstructing benign prostatic hyperplasia (BPH) has been transurethral resection of the prostate (TURP). With the development of new laser technology minimally invasive surgical procedures have been introduced in an attempt to decrease the morbidity experienced with standard TURP. Laser treatment of BPH has evolved from coagulation to complete adenoma enucleation. The holmium laser was initially utilized for prostate ablation and soon evolved into holmium laser tissue resection, but was limited by difficulties with extracting the prostate tissue from the bladder. With the development of a compatible tissue morcellator whole prostate lobes could be enucleated similar to an open prostate enucleation and the HoLEP procedure was developed. Currently HoLEP is the only procedure to demonstrate superior outcomes to TURP on urodynamic studies and long-term studies demonstrate its durability up to 7 years post procedure. Changes in enucleation technique have also increased the efficiency of the HoLEP procedure, such that any sized prostate can be treated. Conclusions: HoLEP is a safe and effective surgical treatment for symptomatic BPH, dependent on a high powered laser and morcellation system. The procedure continues to gain acceptance due to excellent short and long-term results, its wide application, and further simplification of technique. PMID:21116363

  5. Sequential transurethral enucleation of the prostate and laparoscopic bladder diverticulectomy.

    Science.gov (United States)

    Yu, Yang; Guan, Sheng; Shen, Chen; Yang, Bo

    2016-08-01

    Transurethral endoscopic technique and standard laparoscopic technique are surgical options for the management of benign prostatic hyperplasia (BPH) associated with urinary bladder diverticuli (UBD). In this article, we report laparoscopic diverticulectomy (LD) and transurethral plasmakinetic enucleation of the prostate (TUEP) in the same patient sequentially. To the best of our knowledge, this is the first case report of LD combined with TUEP. An 82-year-old patient with benign prostatic hyperplasia and two secondary large bladder diverticuli underwent sequential TUEP and LD. After completion of the TUEP procedure, the detached adenoma was pushed into the bladder as a whole. Then laparoscopic transperitoneal extravesical diverticulectomy assisted by cystoscopic transillumination was performed immediately, and the enucleated prostate was removed via the neck of the diverticulum. The enucleation time and diverticulectomy time was 18 minutes and 108 minutes, respectively. The catheter was removed on the tenth postoperative day. Transurethral endoscopic surgery combined with LD is a good choice in treating BPH and UBD in one session. But the combined procedure is time-consuming, especially for fragmentation of the prostate. TUEP can greatly reduce the operative time of the combined procedure.

  6. Calcium Signaling Is Required for Erythroid Enucleation.

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    Christina B Wölwer

    Full Text Available Although erythroid enucleation, the property of erythroblasts to expel their nucleus, has been known for 7ore than a century, surprisingly little is known regarding the molecular mechanisms governing this unique developmental process. Here we show that similar to cytokinesis, nuclear extrusion requires intracellular calcium signaling and signal transduction through the calmodulin (CaM pathway. However, in contrast to cytokinesis we found that orthochromatic erythroblasts require uptake of extracellular calcium to enucleate. Together these functional studies highlight a critical role for calcium signaling in the regulation of erythroid enucleation.

  7. Impaled Orbital TASER Probe Injury Requiring Primary Enucleation.

    Science.gov (United States)

    Rafailov, Leon; Temnogorod, Jenny; Tsai, Frank F; Shinder, Roman

    The Conducted Electrical Weapon is a weapon often used by law enforcement agencies as a method of less lethal means to subdue a suspect. Injuries to the eye with these devices are usually due to the projectile force of the metal probes that are released when these devices are engaged. The authors report what may be the first case of an impaled orbital TASER probe that required primary enucleation for globe perforation.

  8. Treatment of a large radicular cyst - enucleation or decompression?

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    Matijević Stevo

    2015-01-01

    Full Text Available Introduction. Radicular cysts treatment involves surgical approach, more or less aggressive. However, treatment of large cystic lesions, including radicular cysts, causes some of dilemmas concerning the choice of the surgical method, especially the degree of radicalism. Case report. We presented a 65-year-old male patient with large radicular cyst in the mandible. A large elliptical multilocular radiolucency, located in the left side of the mandible, being in close vicinity to the mandibular canal, was registered at the orthopantomographic radiography. There was a risk of pathological fracture of the mandible. However, the cyst was completely removed by enucleation without intraoperative and postoperative complications. Conclusion. The presented case support the opinion that careful enucleation of large mandibular cysts may be done without complications, such as damages of surrounding anatomical structures or mandibular fracture. The authors indicate reasons for strong support of the undertaken surgical approach of treating large radicular cysts in the mandible.

  9. Treatment of a large radicular cyst-enucleation or decompression?

    Science.gov (United States)

    Matijević, Stevo; Jovivić, Bojan; Bubalo, Marija; Dukić, Smiljka; Cutović, Tatjana

    2015-04-01

    Radicular cysts treatment involves surgical approach, more or less aggressive. However, treatment of large cystic lesions, including radicular cysts, causes some of dilemmas concerning the choice of the surgical method, especially the degree of radicalism. We presented a 65-year-old male patient with large radicular cyst in the mandible. A large elliptical multilocular radiolucency, located in the left side of the mandible, being in close vicinity to the mandibular canal, was registered at the orthopantomographic radiography. There was a risk of pathological fracture of the mandible. However, the cyst was completely removed by enucleation without intraoperative and postoperative complications. The presented case support the opinion that careful enucleation of large mandibular cysts may be done without complications, such as damages of surrounding anatomical structures or mandibular fracture. The authors indicate reasons for strong support of the undertaken surgical approach of treating large radicular cysts in the mandible.

  10. From "gold standard" resection to reproducible "future standard" endoscopic enucleation of the prostate: what we know about anatomical enucleation.

    Science.gov (United States)

    Naspro, Richard; Gomez Sancha, Fernando; Manica, Michele; Meneghini, Agostino; Ahyai, Sascha; Aho, Tevita; Fiori, Cristian; Vavassori, Ivano; DA Pozzo, Luigi F; Pansadoro, Vito; Montorsi, Francesco; Herrmann, Thomas R

    2017-10-01

    Open prostatectomy (OP) and transurethral resection of the prostate (TURP) have traditionally been the most common surgical approaches for the treatment of benign prostatic hyperplasia causing bladder outlet obstruction and have certainly passed the test of time. In time, many endoscopic surgical procedures have been described as an alternative mini-invasive treatment. Holmium laser enucleation (HoLEP) guaranteed functional outcomes similar to OP and TURP with lower perioperative complication rates for any prostate size. With the development of different kinds of lasers (such as thulium, "green light" and diode) and bipolar energy, the feasibility of endoscopic enucleation using these energies has been explored. In this paper, recent techniques to perform true prostate enucleation have been reviewed through a search of PubMed and Web of Science, including articles published in the last 20 years in clinical journals. The review is based on a peer-review process of the authors after a structured data search. Search terms included "Thulium prostate enucleation, THULEP, TmLEP/Tm Yag enucleation" OR "Greenlight enucleation/prostate enucleation/vapo-enucleation/KTP prostate enucleation, PVP prostate enucleation, GreenLep/" OR "bipolar prostate enucleation" OR "HoLEP, Holmium prostate enucleation" OR "monopolar prostate enucleation" OR "Diode prostate enucleation" OR "DiLEP" OR "Eraser prostate enucleation" OR "ELEP". Following the example of HoLEP, many techniques have been described in the literature using a variety of energy sources and instruments either in a pure enucleative or a hybrid (mixed) fashion. However, the levels of evidence are too low and follow-up still too short to offer solid recommendations. HoLEP has become the conceptual and practical paradigm for the wide spread of enucleation thanks to the evidence provided by the literature and excellent outcomes. Higher level of evidence is required to assess efficacy of alternative enucleative techniques.

  11. Osmotically-assisted desalination method and system

    Science.gov (United States)

    Achilli, Andrea; Childress, Amy E.; Cath, Tzahi Y.

    2014-08-12

    Systems and methods for osmotically assisted desalination include using a pressurized concentrate from a pressure desalination process to pressurize a feed to the desalination process. The depressurized concentrate thereby produced is used as a draw solution for a pressure-retarded osmosis process. The pressure-retarded osmosis unit produces a pressurized draw solution stream that is used to pressurize another feed to the desalination process. In one example, the feed to the pressure-retarded osmosis process is impaired water.

  12. [Traumatic enucleation. Accident or self-mutilation?].

    Science.gov (United States)

    Straßburger, P; Váradi, G

    2013-05-01

    A 52-year-old woman was admitted for emergency treatment after traumatic enucleation. Because of the position of the avulsed eye and the acute mental condition of the patient a trauma caused by self-mutilation could not be excluded. There was even the suspicion of self-enucleation in a second case and in both cases a psychiatrist was consulted but ultimately a disastrous accident was clearly verified for both patients. Nevertheless, an interdisciplinary evaluation should be initiated in cases of patients with a suspicious traumatic injury as in cases of self-mutilation the danger of relapse is high in the first hours and weeks after the primary event.

  13. [Transurethral vapor enucleation and resection of prostate with TURis button electrode].

    Science.gov (United States)

    Xie, Li-ping; Qin, Jie; Zheng, Xiang-yi; Mao, Qi-qi; Zhang, Peng; Huang, Xiao; Tan, Fu-qing; Liu, Ben

    2012-06-12

    To describe an efficient and effective method of using Olympus TURis button plasma vaporization electrode plus loop electrode for transurethral vapor enucleation and resection of prostate. Between July 2011 and October 2011, the investigators performed transurethral vapor enucleation and resection of prostate using Olympus TURis button plasma vaporization electrode plus loop electrode in 16 consecutive patients at our institution. The parameters of prostate weight, International Prostate Symptom Score (IPSS), quality of life (QOL), operative duration, blood loss volume, catheterization period, duration of hospitalization, perioperative complications and the weight of enucleated tissue were evaluated. IPSS and QOL were recorded during the follow-up. No patient had significant blood loss or signs of transurethral resection syndrome. The mean patient age was 67.3 ± 8.1 years. Mean preoperative prostate weight was 49 ± 24 g (range: 19 - 91) and mean resected tissue weight 36 ± 16 g (range: 10 - 50). Serious complications were not observed. Operative duration was 116 ± 31 minutes, mean catheter time 4.9 ± 1.8 days and the duration of hospitalization was 16.6 ± 5.5 days. Transurethral vapor enucleation and resection of prostate induced significant, pronounced, immediate and lasting improvement in IPSS (15.6 ± 6.8 vs 6.7 ± 2.4, P Transurethral vapor enucleation and resection of prostate with Olympus TURis plasma button electrode is a safe, effective and thorough surgical method in the treatment of benign prostatic hyperplasia.

  14. [Transurethral holmium enucleation of prostatic adenoma].

    Science.gov (United States)

    Martov, A G; Maksimov, V A; Iarovoĭ, S Iu; Ergakov, D V; Kornienko, S I; Fakhredinov, G A

    2011-01-01

    Laser (holmium) enucleation of the prostate (LEP) for infravesical obstruction caused by prostatic adenoma up to 60 cm3 in size was made in 42 patients in the Moscow city urological hospital N 47 from January to July 2009. Transurethral resection (TUR) of the prostate was made in 42 matched controls. All the patients from both groups have undergone the same standard clinical and laboratory examination before the treatment, 1, 3 and 6 months after it. Duration of surgery was shorter in the TUR group (42.4 +/- 26.1 min vs. 74.8 +/- 39.7 min). Washing system operated longer in the TUR group (23 +/- 7 vs. 10 +/- 5 h). The LEP group patients stayed in the hospital for much shorter time (3.1 +/- 1.2 days vs 4.6 +/- 1.8 days). No significant differences between the groups by basic postoperative parameters were found 1, 3 and 6 months after the operation. Postoperative complications were rare in both groups. Hemorrhagic complications were absent in the LEP group. This makes it possible to recommend LEP for patients with coagulopathy. Trauma of the bladder occurred in 1 case of LEP because of a complicated technique of LEP and the absence of conventional endoscopic reference points characteristic for TUR. Thus, our initial experience with transurethral LEP in comparison with TUR leads us to the conclusion that LEP is effective and safe in surgical treatment of patients with prostatic adenoma. Further gain of experience with LEP conduction may make this method alternative to open adenomectomy.

  15. Office Hysteroscopic Laser Enucleation of Submucous Myomas without Mass Extraction: A Case Series Study

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    Sergio Haimovich

    2015-01-01

    Full Text Available Background and Objectives. A new two-step hysteroscopic myomectomy carried out in the office setting and without anesthesia was feasible for the excision of submucous myomas. The objective of this study was to assess whether removal of submucous myomas from the uterine cavity after hysteroscopic laser enucleation is necessary. Methods. Between June 2009 and April 2013, all outpatients with symptomatic myomatosis (bleeding, pelvic pain, and infertility assessed ultrasonographically were eligible to participate in a prospective study. All patients underwent office hysteroscopic enucleation of submucous myomas. Enucleated myomas were left in the uterine cavity. Neither anesthesia nor antibiotic prophylaxis was used. Results. Sixty-one women (mean age: 47.3 years were included. Regardless of hysteroscopic localization and grading, all myomas were enucleated. The mean (standard deviation, SD diameter of the myoma as measured by the ultrasound scan was 22.6 (8.5 mm. In 29 cases (47.5%, the diameter of the resected myoma was >20 mm and in 10 cases (16.4% >30 mm. After a mean follow-up of 68.2 (16.5 days, none of the patients showed a residual myoma inside the uterine cavity. Conclusions. The present results indicate that leaving laser-enucleated submucous myoma in the uterine cavity is a feasible and safe therapeutic option.

  16. Standard Enucleation with Aluminium Oxide Implant (Bioceramic Covered with Patient's Sclera

    Directory of Open Access Journals (Sweden)

    Gian Luigi Zigiotti

    2012-01-01

    Full Text Available Purpose. We describe in our study a modified standard enucleation, using sclera harvested from the enucleated eye to cover the prosthesis in order to insert a large porous implant and to reduce postoperative complication rates in a phthisis globe. Methods. We perform initially a standard enucleation. The porous implant (Bioceramic is then covered only partially by the patient's sclera. The implant is inserted in the posterior Tenon's space with the scleral covering looking at front. All patients were followed at least for twelve months (average followup 16 months. Results. We performed nineteen primary procedures (19 patients, 19 eyes, M; F and secondary, to fill the orbital cavity in patients already operated by standard evisceration (7 patients, 7 eyes. There were no cases of implant extrusion. The orbital volume was well reintegrated. Conclusion. Our procedure was safe and effective. All patients had a good cosmetic result after final prosthetic fitting and we also achieved good prothesis mobility.

  17. Standard Enucleation with Aluminium Oxide Implant (Bioceramic) Covered with Patient's Sclera

    Science.gov (United States)

    Zigiotti, Gian Luigi; Cavarretta, Sonia; Morara, Mariachiara; Nam, Sang Min; Ranno, Stefano; Pichi, Francesco; Lembo, Andrea; Lupo, Stefano; Nucci, Paolo; Meduri, Alessandro

    2012-01-01

    Purpose. We describe in our study a modified standard enucleation, using sclera harvested from the enucleated eye to cover the prosthesis in order to insert a large porous implant and to reduce postoperative complication rates in a phthisis globe. Methods. We perform initially a standard enucleation. The porous implant (Bioceramic) is then covered only partially by the patient's sclera. The implant is inserted in the posterior Tenon's space with the scleral covering looking at front. All patients were followed at least for twelve months (average followup 16 months). Results. We performed nineteen primary procedures (19 patients, 19 eyes, x M; x F) and secondary, to fill the orbital cavity in patients already operated by standard evisceration (7 patients, 7 eyes). There were no cases of implant extrusion. The orbital volume was well reintegrated. Conclusion. Our procedure was safe and effective. All patients had a good cosmetic result after final prosthetic fitting and we also achieved good prothesis mobility. PMID:22654614

  18. MR imaging of post transurethral enucleation of the prostate for benign prostatic hypertrophy

    Energy Technology Data Exchange (ETDEWEB)

    Tajima, Natsuki; Okada, Susumu [Nippon Medical School, Chiba (Japan). Chiba Hokusoh Hospital; Hiraoka, Yasunori; Tajima, Hiroyuki; Iida, Eiji; Kobayashi, Yuko; Sugizaki, Kenichi; Yamamoto, Kanae; Kumazaki, Tatsuo

    1995-11-01

    Magnetic resonance imaging of post transurethral enucleation of the prostate for benign prostatic hypertrophy was evaluated in 11 patients. Both pre- and post-enucleation images were evaluated in 6 patients. Decrease in maximum diameter of prostatic glands after this method had a good correlation with the resected volume, uroflowmetry analysis and clinical symptoms. Intensity decrease of peripheral zone on T2-weighted image was detected in 3 of 6 patients, while size increase of peripheral zone was seen in 1 of 6 patients. Low intensity band around the resected urethra was demonstrated in all patients after surgical intervention. (author).

  19. Performance Evaluation Methods for Assistive Robotic Technology

    Science.gov (United States)

    Tsui, Katherine M.; Feil-Seifer, David J.; Matarić, Maja J.; Yanco, Holly A.

    Robots have been developed for several assistive technology domains, including intervention for Autism Spectrum Disorders, eldercare, and post-stroke rehabilitation. Assistive robots have also been used to promote independent living through the use of devices such as intelligent wheelchairs, assistive robotic arms, and external limb prostheses. Work in the broad field of assistive robotic technology can be divided into two major research phases: technology development, in which new devices, software, and interfaces are created; and clinical, in which assistive technology is applied to a given end-user population. Moving from technology development towards clinical applications is a significant challenge. Developing performance metrics for assistive robots poses a related set of challenges. In this paper, we survey several areas of assistive robotic technology in order to derive and demonstrate domain-specific means for evaluating the performance of such systems. We also present two case studies of applied performance measures and a discussion regarding the ubiquity of functional performance measures across the sampled domains. Finally, we present guidelines for incorporating human performance metrics into end-user evaluations of assistive robotic technologies.

  20. Irreversible electroporation of human primary uveal melanoma in enucleated eyes.

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    Yossi Mandel

    Full Text Available Uveal melanoma (UM is the most common primary intraocular tumor in adults and is characterized by high rates of metastatic disease. Although brachytherapy is the most common globe-sparing treatment option for small- and medium-sized tumors, the treatment is associated with severe adverse reactions and does not lead to increased survival rates as compared to enucleation. The use of irreversible electroporation (IRE for tumor ablation has potential advantages in the treatment of tumors in complex organs such as the eye. Following previous theoretical work, herein we evaluate the use of IRE for uveal tumor ablation in human ex vivo eye model. Enucleated eyes of patients with uveal melanoma were treated with short electric pulses (50-100 µs, 1000-2000 V/cm using a customized electrode design. Tumor bioimpedance was measured before and after treatment and was followed by histopathological evaluation. We found that IRE caused tumor ablation characterized by cell membrane disruption while sparing the non-cellular sclera. Membrane disruption and loss of cellular capacitance were also associated with significant reduction in total tumor impedance and loss of impedance frequency dependence. The effect was more pronounced near the pulsing electrodes and was dependent on time from treatment to fixation. Future studies should further evaluate the potential of IRE as an alternative method of uveal melanoma treatment.

  1. Brain-controlled body movement assistance devices and methods

    Energy Technology Data Exchange (ETDEWEB)

    Leuthardt, Eric C.; Love, Lonnie J.; Coker, Rob; Moran, Daniel W.

    2017-01-10

    Methods, devices, systems, and apparatus, including computer programs encoded on a computer storage medium, for brain-controlled body movement assistance devices. In one aspect, a device includes a brain-controlled body movement assistance device with a brain-computer interface (BCI) component adapted to be mounted to a user, a body movement assistance component operably connected to the BCI component and adapted to be worn by the user, and a feedback mechanism provided in connection with at least one of the BCI component and the body movement assistance component, the feedback mechanism being configured to output information relating to a usage session of the brain-controlled body movement assistance device.

  2. Use of a hydroxyapatite orbital implant in a cosmetic corneoscleral prosthesis after enucleation in a horse.

    Science.gov (United States)

    Gilger, Brian C; Pizzirani, Stefano; Johnston, Lisa C; Urdiales, Nicholas R

    2003-02-01

    A hydroxyapatite orbital implant was used after enucleation of an eye from a 5-year-old performance horse. A custom-made corneoscleral prosthesis was made and fitted over the hydroxyapatite implant. The implant and surgery were well tolerated. Placement of a cosmetic prosthesis is desired after enucleation of equine eyes to allow horses to return to competition. Synthetic spheres consisting of methylmethacrylate or silicone have been used, although reported complications have included extrusion, infection, and poor cosmetic results. Hydroxyapatite orbital implants made from marine coral allow vascular and fibrous tissue growth from the host orbit into the implant, which decreases the possibility of implant extrusion or infection and allows enhanced healing of defects in the overlying conjunctiva. Extraocular muscle fixation onto the implant permits motility and assists in the prevention of implant extrusion.

  3. JAW CYSTS AND GUIDED BONE REGENERATION (a late complication after enucleation

    Directory of Open Access Journals (Sweden)

    Hristina Lalabonova

    2013-10-01

    Full Text Available Maxillary jaw bone possesses a high regenerative capacity. Yet sometimes the defects enucleation of jaw cysts leaves may regenerate only partially or not at all. For this reason some researchers advise treatment of the residual cavities after cystectomy using bone regeneration stimulation methods. We report a case of an atypical complication after enucleation of a maxillary cyst manifesting itself eight years after the initial treatment. The symptoms the patient reported were at first periodic sweating on the left sides of face and head. This was followed by a piercing pain in the left palpebral fissure radiating to the middle of the palate and felt in the left cheekbone, left eye and left supraorbital ridge. The patient has a history of maxillary cysts recurring three times and of three operations she had 20, 12 and 8 years previously. The multiple recurrences of the cysts after their enucleation indicates poor regenerative capacity of the body which resulted in the formation of cicatricial tissue. It is most probably this tissue that was responsible for the disruption of the nerve conduction capacity which can account for the reported symptoms. We filled the cavity with bone graft material which boosted the bone structure regeneration. Although maxillary jaws possess high regenerative capacity we advise the use of guided bone regeneration in cases of large bone defects that usually occur after enucleation of jaw cysts.

  4. Psychosocial factors influencing parental decision to allow or refuse potentially lifesaving enucleation in children with retinoblastoma

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    Rolando Enrique D. Domingo

    2017-01-01

    Full Text Available Objective: Retinoblastoma is the most common malignancy of the eye and ocular adnexa in the Philippines. It is curable when treated early, but delay in enucleation is common due to the parental refusal of surgery for varied reasons. The aim of this study is to identify the psychosocial barriers and facilitating factors for accepting versus refusing enucleation as treatment for retinoblastoma. Methods: This is a cross-sectional descriptive study utilizing structured interviews and a questionnaire. It was conducted at the Retinoblastoma Clinic of the Philippine General Hospital. A questionnaire using the Likert scale was constructed after performing key informant interviews and focus group discussions. It was pretested and revised before parents of patients with retinoblastoma were invited to participate in the study. Descriptive statistics, quantitative item analyses using inter-item correlations and item-total correlations was performed. Results: Factors that correlate with refusal to enucleate are the beliefs that cancer is a fatal illness, the fear of unacceptable esthetic outcome of the surgery, and the cost of treatment. Favorable factors include value of life, high regard for the opinion of medical practitioners, and appreciation of the efficacy of treatment. Conclusions: There are several favorable factors and barriers that health practitioners must consider in facilitating parental decision-making toward enucleation for retinoblastoma.

  5. Novel methylene blue staining technique for localizing small esophageal leiomyomas during thoracoscopic enucleation.

    Science.gov (United States)

    Zhang, Z; Ai, B; Liao, Y; Liu, L; Liu, M

    2016-11-01

    The treatment of choice for leiomyoma, the most common benign esophageal tumor, is thoracoscopic enucleation. One of the most difficult aspects of thoracoscopic enucleation is the precise localization of small tumors (≤1.5 cm) and tumors without external protrusion. No simple, feasible solutions to this problem are available. We developed a novel methylene blue staining technique to localize small esophageal leiomyomas and evaluated the feasibility of our technique. Between January 2013 and July 2014, eight patients with small esophageal leiomyomas (≤1.5 cm) underwent thoracoscopic enucleation in Tongji Hospital. Preoperative endoscopic ultrasonography was performed in all patients. The leiomyomas were located in the middle (n = 5) and lower (n = 3) thirds of the esophagus. We preoperatively injected 0.5-1.0 mL methylene blue in the submucosa adjacent to the tumors under standard gastroscope guidance. The entire staining process took about 10 minutes. Staining was successful in all patients. The unstained tumor was exposed after the blue-stained mediastinal pleura, and overlying muscle were incised longitudinally. All procedures were successfully completed without conversion to open surgery. No abnormalities were detected in the esophageal mucosa. The median operating time was 60 minutes (range, 40-90 minutes). Postoperative histopathology confirmed leiomyoma in all patients. The median postoperative hospital stay was 6 days (range, 5-7 days). No major complications, such as esophageal leakage or esophageal diverticulum, occurred. Endoscopic methylene blue staining is safe and feasible for localizing small esophageal leiomyomas during thoracoscopic enucleation. This method will enable precise and easy enucleation. © 2015 International Society for Diseases of the Esophagus.

  6. Research advances in control methods of wearable walking assist robots

    Directory of Open Access Journals (Sweden)

    Xia ZHANG

    2016-04-01

    Full Text Available As the proportion of the elderly in China increases, the need for robotic assist walking is growing. The assisted-as-needed (AAN property of a wearable walking assist robot matches a user’s biological need and improves the flexibility, appetency and friendliness of a mechanical system. To realize AAN walking and aiming at realizing master/slave flexible assist, a new hybrid control method consisting of hip joint control based on central pattern generators and knee joint impedance structured control is proposed. The adaptation of a robot's master/slave motion mode to a user's physical function, the continuous switching method for knee joint impedance structured control and its stability, and the AAN effect of the Hybrid control theory are studied, which provides a new thought for the development of wearable walking assist robots.

  7. Microwave assisted spectrophotometric method for the ...

    African Journals Online (AJOL)

    A sensitive spectrophotometric method has been developed for the trace determination of copper using leucocrystal violet as chromogenic reagent. The proposed method is based on the reaction of copper(II) with potassium iodide in acid medium to liberate iodine, which oxidizes leucocrystal violet to crystal violet dye ...

  8. Microcomputer-Assisted Mathematics. Roots: Newton's Method.

    Science.gov (United States)

    Kimberling, Clark

    1985-01-01

    Discusses Newton's method for approximating the roots of functions, indicating that students who program in BASIC can learn and appreciate the method by writing their own programs. Includes a hypothetical dialogue between teacher and student about the topic; sample program listings; and problems assigned to students who have written programs. (JN)

  9. Formation of mammalian erythrocytes: chromatin condensation and enucleation.

    Science.gov (United States)

    Ji, Peng; Murata-Hori, Maki; Lodish, Harvey F

    2011-07-01

    In all vertebrates, the cell nucleus becomes highly condensed and transcriptionally inactive during the final stages of red cell biogenesis. Enucleation, the process by which the nucleus is extruded by budding off from the erythroblast, is unique to mammals. Enucleation has critical physiological and evolutionary significance in that it allows an elevation of hemoglobin levels in the blood and also gives red cells their flexible biconcave shape. Recent experiments reveal that enucleation involves multiple molecular and cellular pathways that include histone deacetylation, actin polymerization, cytokinesis, cell-matrix interactions, specific microRNAs and vesicle trafficking; many evolutionarily conserved proteins and genes have been recruited to participate in this uniquely mammalian process. In this review, we discuss recent advances in mammalian erythroblast chromatin condensation and enucleation, and conclude with our perspectives on future studies. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. Unsuccessful Self-Enucleation in a Schizophrenic Patient

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    Noam Bar-Yaakov

    2014-01-01

    Full Text Available Self-enucleation is a very unusual form of self-mutilation directly linked to mental illness. In this case we present a 26-year-old schizophrenic patient who attempted to enucleate his eye with a rollerball pen. Antipsychotic therapy and emergency surgery saved the patient eye and emphasize the importance of quick response and good collaboration between psychiatric and ophthalmic teams.

  11. Shifts in developmental timing, and not increased levels of experience-dependent neuronal activity, promote barrel expansion in the primary somatosensory cortex of rats enucleated at birth.

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    Ingrid Fetter-Pruneda

    Full Text Available Birth-enucleated rodents display enlarged representations of whiskers (i.e., barrels of the posteromedial subfield in the primary somatosensory cortex. Although the historical view maintains that barrel expansion is due to incremental increases in neuronal activity along the trigeminal pathway during postnatal development, recent evidence obtained in experimental models of intramodal plasticity challenges this view. Here, we re-evaluate the role of experience-dependent neuronal activity on barrel expansion in birth-enucleated rats by combining various anatomical methods and sensory deprivation paradigms. We show that barrels in birth-enucleated rats were already enlarged by the end of the first week of life and had levels of metabolic activity comparable to those in control rats at different ages. Dewhiskering after the postnatal period of barrel formation did not prevent barrel expansion in adult, birth-enucleated rats. Further, dark rearing and enucleation after barrel formation did not lead to expanded barrels in adult brains. Because incremental increases of somatosensory experience did not promote barrel expansion in birth-enucleated rats, we explored whether shifts of the developmental timing could better explain barrel expansion during the first week of life. Accordingly, birth-enucleated rats show earlier formation of barrels, accelerated growth of somatosensory thalamocortical afferents, and an earlier H4 deacetylation. Interestingly, when H4 deacetylation was prevented with a histone deacetylases inhibitor (valproic acid, barrel specification timing returned to normal and barrel expansion did not occur. Thus, we provide evidence supporting that shifts in developmental timing modulated through epigenetic mechanisms, and not increased levels of experience dependent neuronal activity, promote barrel expansion in the primary somatosensory cortex of rats enucleated at birth.

  12. Discharge and infection in retinoblastoma post-enucleation sockets

    Directory of Open Access Journals (Sweden)

    Mourits DL

    2017-03-01

    Full Text Available Daphne L Mourits,1 Dyonne T Hartong,1 Andries E Budding,2 Machteld I Bosscha,1 H Stevie Tan,1 Annette C Moll1 1Department of Ophthalmology, VU University Medical Center, 2Department of Microbiology, Academic Medical Center, Amsterdam, the Netherlands Purpose: To investigate the causes and treatment options for socket discharge and infection in patients enucleated for retinoblastoma (Rb. Methods: A questionnaire was filled out by (parents of ocular prosthesis-wearing patients with a history of enucleation as treatment for Rb. We collected data on patients’ characteristics, cleaning habits of the prosthesis, frequency of socket irritation, discharge, and infection, and use of antibiotics. With ordinal logistic regression analysis, factors related to the outcome parameters (frequency of irritation, mucoid and purulent discharge were identified. In a subset of young asymptomatic and symptomatic patients, a swab culture of the socket was performed to determine the presence of microorganisms. Results: A total of 186 patients or their parents (mean age of the patients: 17.3 years, ranging from 0.8 to 88.3 years filled out the questionnaire. Irritation, mucoid discharge, and purulent discharge were frequently (once a month or more often experienced in 75 (39.5%, 127 (66.8%, and 15 (13.2% sockets, respectively. Younger age was associated with a higher frequency of mucoid and purulent discharge. Radiation therapy, chemotherapy, gender, age at surgery, cleaning frequency, and nocturnal wear were not associated with the outcome parameters. In a subgroup of 26 patients, the sockets were swabbed and cultured. All symptomatic patients had a positive bacterial culture versus 15% (2/13 of the asymptomatic patients (P<0.001. Common cold was correlated with both symptoms and presence of bacteria. Haemophilus influenzae and Staphylococcus aureus were the species most frequently cultured. Conclusion: Ocular prosthesis-wearing patients often experienced mucoid

  13. [Transurethral plasmakinetic enucleation of the prostate for benign prostatic hyperplasia].

    Science.gov (United States)

    Sheng, Xu-jun; Chen, Jian-hua; Wang, Wei-ming; Kong, Liang; Zhang, Liang; Yu, Yong-jiang; Wu, Yu; Qi, Jun

    2011-05-01

    To evaluate clinical application of transurethral plasmakinetic enucleation of the prostate (PKEP) to the treatment of benign prostatic hyperplasia (BPH). A total of 90 BPH patients, aged 59-83 (mean 71) years and with indication of surgery, underwent transurethral resection of the prostate (the TURP group, n=50) and transurethral plasmakinetic enucleation of the prostate (the PKEP group, n=40), respectively. We recorded and analyzed the preoperative prostate volume, IPSS, QOL and Qmax, operation time, intra- and post-operative bleeding and complications, postoperative continuous bladder irrigation, and IPSS, QOL and Qmax at 2 weeks and 6 months after surgery. The preoperative prostate volume and operation time were 58.9 g and 58.8 min in the TURP group versus 58.3 g and 93.0 min in the PKEP group. Mild transurethral resection syndrome (TURS) appeared in 2 TURP receivers, while no abnormality was found in electrocardiogram monitoring in those undergoing PKEP. Continuous bladder irrigation was necessitated in 3 and urgent incontinence of urine occurred in 4 cases of TURP, as compared with 1 and 4 cases in the PKEP group. None of the 90 patients needed blood transfusion. At 2 weeks before and after surgery and 6 months postoperatively, IPSS averaged 19.7, 11.6 and 5.1, QOL 4.6, 3.3 and 1.1, and Qmax 6.3, 13.0 and 18.1 ml/s in the TURP group versus 18.6, 8.4 and 4.9 (IPSS), 4.5, 2.7 and 1.1 (QOL) and 6.9, 14.2 and 19.0 ml/s (Qmax) in the PKEP group. There were significant differences in operation time, IPSS and QOL at 2 weeks postoperatively between the two groups, as well as in IPSS, QOL and Qmax at 6 months before and after surgery (P 0.01). Transurethral PKEP is a safe, effective and thorough surgical method to be chosen for the treatment of BPH.

  14. Mouse eye enucleation for remote high-throughput phenotyping.

    Science.gov (United States)

    Mahajan, Vinit B; Skeie, Jessica M; Assefnia, Amir H; Mahajan, Maryann; Tsang, Stephen H

    2011-11-19

    The mouse eye is an important genetic model for the translational study of human ophthalmic disease. Blinding diseases in humans, such as macular degeneration, photoreceptor degeneration, cataract, glaucoma, retinoblastoma, and diabetic retinopathy have been recapitulated in transgenic mice.(1-5) Most transgenic and knockout mice have been generated by laboratories to study non-ophthalmic diseases, but genetic conservation between organ systems suggests that many of the same genes may also play a role in ocular development and disease. Hence, these mice represent an important resource for discovering new genotype-phenotype correlations in the eye. Because these mice are scattered across the globe, it is difficult to acquire, maintain, and phenotype them in an efficient, cost-effective manner. Thus, most high-throughput ophthalmic phenotyping screens are restricted to a few locations that require on-site, ophthalmic expertise to examine eyes in live mice. (6-9) An alternative approach developed by our laboratory is a method for remote tissue-acquisition that can be used in large or small-scale surveys of transgenic mouse eyes. Standardized procedures for video-based surgical skill transfer, tissue fixation, and shipping allow any lab to collect whole eyes from mutant animals and send them for molecular and morphological phenotyping. In this video article, we present techniques to enucleate and transfer both unfixed and perfusion fixed mouse eyes for remote phenotyping analyses.

  15. ASPECTS: an automation-assisted SPE method development system.

    Science.gov (United States)

    Li, Ming; Chou, Judy; King, Kristopher W; Yang, Liyu

    2013-07-01

    A typical conventional SPE method development (MD) process usually involves deciding the chemistry of the sorbent and eluent based on information about the analyte; experimentally preparing and trying out various combinations of adsorption chemistry and elution conditions; quantitatively evaluating the various conditions; and comparing quantitative results from all combination of conditions to select the best condition for method qualification. The second and fourth steps have mostly been performed manually until now. We developed an automation-assisted system that expedites the conventional SPE MD process by automating 99% of the second step, and expedites the fourth step by automatically processing the results data and presenting it to the analyst in a user-friendly format. The automation-assisted SPE MD system greatly saves the manual labor in SPE MD work, prevents analyst errors from causing misinterpretation of quantitative results, and shortens data analysis and interpretation time.

  16. Integrating medical, assistive, and universal design products and technologies: Assistive Technology Service Method (ATSM).

    Science.gov (United States)

    Elsaesser, Linda-Jeanne; Bauer, Stephen

    2012-07-01

    ISO26000 provides guidance on effective organizational performance that recognizes social responsibility (including rights of persons with disabilities (PWD)), engages stakeholders, and contributes to sustainable development [1]. Millennium Development Goals 2010 state: while progress has been made, insufficient dedication to sustainable development, and inequalities to the most vulnerable people require attention [2]. World Report on Disability 2011 recommendations includes improved data collection and removal of barriers to rehabilitation that empower PWD [3]. The Assistive Technology Service Method (ATSM), Assistive Technology Device Classification (ATDC) and Matching Person and Technology (MPT) provide an evidence-based, standardized, internationally comparable framework to improve rehabilitation interventions [4-6]. The ATSM and ATDC support universal design (UD) principles and provision of universal technology. The MPT assures interventions are effective and satisfactory to end-users [7]. The ICF conceptual framework and common language are used throughout [8]. Research findings on healthcare needs are translated. ATSM applications in support of these findings are presented. National initiatives demonstrate the need and value of the ATSM as an evidence-based, user-centric, interdisciplinary method to improve individual and organizational performance for rehabilitation [including AT] services. Two Disability & Rehabilitation: Assistive Technology articles demonstrate ATSM and ATDC use to strengthen rehabilitation services and integrate Universal Design principles for socially responsible behavior.

  17. [Clinical evaluation of transurethral enucleation with bipolar (TUEB)].

    Science.gov (United States)

    Okugi, Hironobu; Makino, Takeaki; Kato, Haruo; Oyam, Yusuke; Okazaki, Hiroshi; Nakamur, Toshiyuki

    2011-01-01

    Transurethral enucleation with bipolar (TUEB) is an established procedure for treating patients with benign prostatic hyperplasia (BPH). In this study, the usefulness of TUEB was evaluated by comparing the preoperative and postoperative results. A total of 45 patients with symptomatic BPH underwent TUEB at our hospital between February 2008 and August 2009. All the patients were assessed using the International prostate symptom score (IPSS), quality of life (QOL) index, overactive bladder symptom score (OABSS), maximum urinary flow rate (Qmax), post void urine residue (PVR), serum prostate specific antigen (PSA) measurement, and estimated prostate weight. Total operative time, enucleation time, enucleated tissue weight, changes in levels of hemoglobin, hematocrit and serum sodium, duration of urethral catheterization, and complications were also investigated. IPSS, QOL index, Qmax, and PVR were reassessed 1, 3, 6 and 12 months after surgery. PSA reduction rate was also assessed 3 months after surgery. The average age was 71.6 years (60-84 years), estimated prostate weight 53.2 g (11.8-105.6 g), total operative time 125.5 minutes (76-212 min.), enucleation time 96.1 min (56-169 min), enucleated tissue weight 32.3 g (6-82 g), and duration of urethral catheterization 2.5 days (2-7 days). Blood transfusion was not required and hyponatremia was not observed in any patients. IPSS, QOL index, OABSS, PVR, and Qmax were significantly improved 1 month after surgery. As complications, re-catheterisation was reported in 2 patients (4.4%), bladder tanponade in 1 (2.2%), and urinary incontinence requiring at least 1 pad 6 months after surgery in 1 (5.9%). Postoperative PSA declined by 89.8% 3 months after surgery. TUEB was a safe and effective treatment procedure for patients with BPH.

  18. Developing Teaching Material Software Assisted for Numerical Methods

    Science.gov (United States)

    Handayani, A. D.; Herman, T.; Fatimah, S.

    2017-09-01

    The NCTM vision shows the importance of two things in school mathematics, which is knowing the mathematics of the 21st century and the need to continue to improve mathematics education to answer the challenges of a changing world. One of the competencies associated with the great challenges of the 21st century is the use of help and tools (including IT), such as: knowing the existence of various tools for mathematical activity. One of the significant challenges in mathematical learning is how to teach students about abstract concepts. In this case, technology in the form of mathematics learning software can be used more widely to embed the abstract concept in mathematics. In mathematics learning, the use of mathematical software can make high level math activity become easier accepted by student. Technology can strengthen student learning by delivering numerical, graphic, and symbolic content without spending the time to calculate complex computing problems manually. The purpose of this research is to design and develop teaching materials software assisted for numerical method. The process of developing the teaching material starts from the defining step, the process of designing the learning material developed based on information obtained from the step of early analysis, learners, materials, tasks that support then done the design step or design, then the last step is the development step. The development of teaching materials software assisted for numerical methods is valid in content. While validator assessment for teaching material in numerical methods is good and can be used with little revision.

  19. Common trend: move to enucleation-Is there a case for GreenLight enucleation? Development and description of the technique.

    Science.gov (United States)

    Gomez Sancha, Fernando; Rivera, Vanesa Cuadros; Georgiev, Georgi; Botsevski, Alexander; Kotsev, Julian; Herrmann, Thomas

    2015-04-01

    Transurethral laser prostatectomy has evolved as a viable alternative for the management of benign prostate enlargement. Since the renaissance of laser prostatectomy with the advent of the holmium:yttrium-aluminum-garnet laser in the 1990s, various lasers and subsequent procedures have been introduced. These techniques can be categorized as vaporizing, resecting, and enucleating approaches. Photoselective vaporization of the prostate (PVP) is dominated by high-power lithium triborate (LBO) crystal lasers (GreenLight XPS). The mainstay of this technique is for the treatment of small to medium prostate volumes whereas enucleating techniques, such as holmium laser enucleation of the prostate and thulium enucleation of the prostate, focus on large-volume glands. In order to perspectively "delimit" LBO into the field of large-volume prostates, we developed LBO en bloc enucleation to render it as a competing transurethral enucleating approach. We present a detailed stepwise progressive technique developed in Madrid, Spain, for the complete removal of the transitional zone by vapoenucleation. The steps include exposition of the prostatic capsule by PVP toward the peripheral zone, thereby identifying the anatomical limits of enucleation. Subsequently, the transitional zone is excised in a single bloc and morcellated after its placement into the bladder. This new GreenLight en bloc enucleation technique allows to treat larger prostates than those previously treated with the PVP technique.

  20. Transurethral enucleation of large benign prostatic hyperplasia in sodium chloride solution

    Directory of Open Access Journals (Sweden)

    I. N. Shvarev

    2016-01-01

    Full Text Available Background. Transurethral resection is a generally recognized treatment for small to medium-sized benign prostatic hyperplasia (BPH. Numerous original tools are proposed for surgery of large BPH. These include laser (holmium laser enucleation of the prostate and transurethral (transurethral enucleation with bipolar (TUEB enucleation in 0.9 % NaCl solution, which are accompanied by removal of the maximum adenoma tissue volume, short-term recovery, and a substantially fewer early and late complications.Objective: to evaluate the safety and efficiency of TUEB in patients with large BPH.Subjects and methods. TUEB was carried out in 58 patients with large BPH. Their mean age was 64 years. The gland size was ≥ 102 сm3. The residual urine volume in all the patients was more than 100 ml. One year later, the results of treatment were analyzed using the International Prostate Symptom Score (IPSS, Quality of Life (QoL Questionnaire, as well as from residual urine volume and remaining prostate tissue.Results. The mean time of surgery and enucleation was 90 and 55 min, respectively. The mean volume of removed adenoma tissue was 99 ml. By the end of month 2 following TUEB, there was a progressive reduction in the total IPSS and QoL scores from 35 to 7 and from 5 to 1, respectively, which preserved this trend by the end of a 1-year follow-up. study. The residual urine amount was less than 30 ml. The mean volume of the prostate tissue left was 8.5 сm3 and less than 11.0 сm3. The ultrasound anatomy of the prostatic urethra (transformation of thin-walled spherical TUR defect that is characteristic slit-like one resembles the inverted letter Y eventually formed by the end of the 1-year follow-up. Stricture of the urethra and contracture of the bladder neck were noted in none of the cases. Conclusion. TUEB is an alternative to open surgery in patients with the enlarged prostate. The use of isotonic NaCl solution as washing fluid allows one to plan

  1. Comparison of the efficacy and feasibility of laser enucleation of bladder tumor versus transurethral resection of bladder tumor: a meta-analysis.

    Science.gov (United States)

    Yang, Huan; Wang, Ning; Han, Shanfu; Male, Musa; Zhao, Chenming; Yao, Daqiang; Chen, Zhiqiang

    2017-12-01

    The transurethral resection of bladder tumor (TURBT) remains the most widely used method in the surgical treatment of the non-muscle invasive bladder tumor (NMIBT). Despite its popularity, the laser technique has been widely used in urology as an alternative, via the application of transurethral laser enucleation of bladder tumor. The aim of the present study was to compare the efficacy and feasibility between transurethral laser enucleation and transurethral resection of bladder tumor. A systematic search of the following databases was conducted: PubMed, Wed of Science, Cochrane Library, EMBASE, Google scholar, and Medline. The search included studies up to the 1st of January 2017. The outcomes of interest that were used in order to assess the two techniques included operation time, catheterization time, hospitalization time, obturator nerve reflex, bladder perforation, bladder irritation, 24-month-recurrence rate, and the postoperative adjuvant intravesical chemotherapy. A total of 13 trials with 2012 participants were included, of which 975 and 1037 underwent transurethral laser enucleation and transurethral resection of bladder tumor, respectively. No significant difference was noted in the operation time between the two groups, although significant differences were reported for the variables catheterization time, hospitalization time, obturator nerve reflex, bladder perforation, bladder irritation, and 24-month-recurrence rate. In the mitomycin and epirubicin subgroups, no significant differences were observed in the laser enucleation and TURBT methods with regard to the 24-month-recurrence rate. The laser enucleation was superior to TURBT with regard to the parameters obturator nerve reflex, bladder perforation, catheterization time, hospitalization time, and 24-month-recurrence rate. Moreover, laser enucleation can offer a more accurate result of the tumor's pathological stage and grade.

  2. [Long-term outcome after endoscopic enucleation of the prostate : From monopolar enucleation to HoLEP and from HoLEP to EEP].

    Science.gov (United States)

    Herrmann, T R W

    2016-11-01

    In the last 20 years various transurethral endoscopic enucleation techniques (EEP) have been established as a substitute for open prostatectomy (OP) and TURP. Since the 2016 update of the "EAU Guidelines on Management of Non-Neurogenic Male Lower Urinary Tract Symptoms (LUTS), including Benign Prostatic Obstruction (BPO)", Holmium laser enucleation of the prostate (HoLEP) and bipolar enucleation being summarized as anatomical enucleating techniques are proposed as first choice for the surgical management of BPO of large volume prostates. The purpose of this review is to demonstrate the available data on long-term outcomes of current EEP techniques. PubMed/Medline and Scopus were searched using the terms: long term, HoLEP, ThuLEP, ThuVEP, DiLEP, ELEP, GreenlEP, Greenlight enucleation, bipolar enucleation, plasmakinetic enucleation, monopolar enucleation, and transurethral enucleation. Studies with a follow-up ≥48 months were selected. In all, 5 randomized controlled trials (2 HoLEP, 2 bipolar enucleation, 1 Thulium laser resection of the prostate in tangerine technique [TmLR-TT]), 3 prospective cohort studies (2 thulium vapoenucleation [ThuVEP], 1 TmLRP-TT), and 2 retrospective studies with large patient cohorts were selected. All EEP were equivalent to OP with regard to effectivity and durability of results. The rate of secondary surgical procedures in HolEP, ThuVEP, bipolar enucleation and tangerine technique (TmLRP-TT) was 0-1.2 % for reTURP, 1.9-3.75 % for urethrotomy, and 0.9-4 % for bladder neck resection. No significant difference in the individual studies was found when compared to OP. For bipolar enucleation vs. TURP long-term results for uroflow, residual urine, and IPSS were significantly better at 60 months for bipolar enucleation. One RCT TmLRP-TT vs. TURP at the 48-month follow-up found no significant difference. Various transurethral EEP can be considered as equally safe and effective anatomical enucleation techniques. All

  3. Monopolar Transurethral Enucleation of Prostatic Adenoma: Preliminary Report.

    Science.gov (United States)

    Pansadoro, Vito; Emiliozzi, Paolo; Del Vecchio, Gianluca; Martini, Marco; Scarpone, Paolo; Del Giudice, Francesco; Veneziano, Domenico; Brassetti, Aldo; Assenmacher, Christophe

    2017-04-01

    To describe preliminary results of our monopolar transurethral enucleation of prostatic adenoma (mTUEPA). A consecutive series of male patients treated with mTUEPA, a retrograde enucleation of the prostatic adenoma performed by means of a standard monopolar resectoscope, were prospectively enrolled. Symptoms, uroflowmetry parameters, and post-voiding residual were assessed at baseline and at 1, 6, and 12 months postoperatively. Prostate volume was evaluated at baseline by means of transrectal ultrasound. Antiplatelet and anticoagulant drugs were stopped at least 1 week before the operation. Forty-seven patients were enrolled. Mean preoperative prostate volume was 64.9 ± 28.5 g. When assessed at baseline, the mean total International Prostatic Symptoms Score was 15.2 ± 3.9, peak flow rate (Qmax) was 8.4 ± 2.9 mL/s and the post-voiding residual was 103.2 ± 90.6 mL. Four weeks after surgery, patients reported a mean International Prostatic Symptoms Score of 5.3 ± 3. This lower urinary tract symptoms relief was further maintained at 6 and 12 months after surgery. A significant postoperative improvement in uroflowmetry parameters was described, being the 6 and 12 months mean Qmax of 23.4 ± 10.6 mL/s and 18.8 ± 9.2 mL/s, respectively (P enucleation and the advantages of mechanical enucleation with standard monopolar transurethral resection of the prostate equipment. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Holmium laser enucleation of the prostate hyperplasia: technical aspects

    Directory of Open Access Journals (Sweden)

    P. V. Glybochko

    2015-01-01

    Full Text Available Holmium laser enucleation of the prostate (HoLEP was first described by doctor P.J. Gilling et al. from New Zealand in 1996. The operation involves anatomical dissection of the prostatic tissue off the surgical capsule using a high-powered holmium laser followed by intravesical morsellation. The objective of this article is to explain the techniques for HoLEP.

  5. Panorama parking assistant system with improved particle swarm optimization method

    Science.gov (United States)

    Cheng, Ruzhong; Zhao, Yong; Li, Zhichao; Jiang, Weigang; Wang, Xin'an; Xu, Yong

    2013-10-01

    A panorama parking assistant system (PPAS) for the automotive aftermarket together with a practical improved particle swarm optimization method (IPSO) are proposed in this paper. In the PPAS system, four fisheye cameras are installed in the vehicle with different views, and four channels of video frames captured by the cameras are processed as a 360-deg top-view image around the vehicle. Besides the embedded design of PPAS, the key problem for image distortion correction and mosaicking is the efficiency of parameter optimization in the process of camera calibration. In order to address this problem, an IPSO method is proposed. Compared with other parameter optimization methods, the proposed method allows a certain range of dynamic change for the intrinsic and extrinsic parameters, and can exploit only one reference image to complete all of the optimization; therefore, the efficiency of the whole camera calibration is increased. The PPAS is commercially available, and the IPSO method is a highly practical way to increase the efficiency of the installation and the calibration of PPAS in automobile 4S shops.

  6. Degradation of 4-chlorophenol by a microwave assisted photocatalysis method.

    Science.gov (United States)

    Ai, Zhihui; Yang, Peng; Lu, Xiaohua

    2005-09-30

    In this work, the degradation of 4-chlorophenol (4CP) under simultaneous microwave assisted UV (electrodeless discharge lamp) photocatalysis technique (MW/UV/TiO2) was investigated. Several factors affecting the degradation of 4CP by MW/UV/TiO2 method, such as the dosage of photocatalysts, the initial pH value of the solutions, gas bubbling, light intensity and addition of H2O2 oxidant, were studied in detail. The synergistic effects between microwave irradiation and TiO2 photocatalysis were also studied. The major intermediates were found to be chlorobenzene, phenol, hydroquinone, benzoquinone and 4-chlorocatechol. Based on the results, a general reaction pathway for the degradation of 4CP was proposed.

  7. Evolving transurethral resection of the prostate: enucleation-resection of the prostate.

    Science.gov (United States)

    Galanakis, Ioannis P; Celia, Antonio; Del Biondo, Dario; Zeccolini, Gugleilmo; Caruso, Adara; Silvestre, Pasquale; Giunta, Angelo; Breda, Guglielmo

    2010-12-01

    Enucleation-transurethral resection of the prostate (e-TURP) is our evolution of the conventional TURP. The aim of this study was to report our experience with e-TURP for the endoscopic management of benign prostatic hyperplasia (BPH). The e-TURP combines the basic steps of classic TURP with the technique of holmium laser enucleation of the prostate (HoLEP) but using only the bipolar resector. The charts of 64 patients who underwent e-TURP for BPH in our department between October 2005 and October 2009 were retrospectively studied. The mean resected tissue weight was 27.21 g, and the mean operative time was 75.7 min. The mean decreases in hemoglobin and serum sodium levels were 1.63 g/dL and 0.55 mEq/L, respectively. Mean catheter duration was 2.53 days, and the mean total hospitalization time was 3.75 days. There was a significant improvement in urinary peak flow rate (Qmax) 2 months postoperatively (P = 0.009718), as well as a significant decrease in the International Prostate Symptom Score during the same period (P < 0.0001). No major complications were observed, and the rates for early and late complications were 10% and 5%, respectively, at 14.75-month mean follow-up. The e-TURP seems to be a safe and effective method for the endoscopic management of BPH. Further investigation with randomized trials is needed on this matter.

  8. Marsupialization and enucleation of keratocystic odontogenic tumor with the use of Carnoy's solution

    Directory of Open Access Journals (Sweden)

    Jovanović Goran

    2010-01-01

    Full Text Available Introduction. Keratocystic odontogenic tumors (KCOT or odontogenic keratocysts are aggressive and expansive odontogenic neoplasms with high recurrence rate (25%- 60%. There are a small number of publications about the combination of marsupialization and enucleation with the use of Carnoy's solution for the treatment of KCOT. Case report. In a female patient, aged 24, marsupialization KCOT was done in the first stage, and enucleation with the use of Carnoy's solution in the second stage, six months later. Lost sensibility of the lower lip was reestablished after three months. A postoperation defect was completely filled in seven months. One year later orthopantomographic x-ray showed the presence of a newly formed bone tissue, whereas in 7 years a completely preserved new mandibular bone and recanalisation of mandibular canal were observed. Conclusion. We consider that our method was successful in the treatment of KCOT, with no occurrence of recidives seven years later. However, it is necessary to follow the patient periodically because of a possible late recidive.

  9. Histone deacetylase 2 is required for chromatin condensation and subsequent enucleation of cultured mouse fetal erythroblasts.

    Science.gov (United States)

    Ji, Peng; Yeh, Victor; Ramirez, Tzutzuy; Murata-Hori, Maki; Lodish, Harvey F

    2010-12-01

    During the final stages of differentiation of mammalian erythroid cells, the chromatin is condensed and enucleated. We previously reported that Rac GTPases and their downstream target, mammalian homolog of Drosophila diaphanous 2 (mDia2), are required for enucleation of in vitro cultured mouse fetal liver erythroblasts. However, it is not clear how chromatin condensation is achieved and whether it is required for enucleation. Mouse fetal liver erythroblasts were purified from embryonic day 14.5 pregnant mice and cultured in erythropoietin-containing medium. Enucleation was determined by flow-cytometry based analysis after treatment with histone deacetylase inhibitors or infection with lentiviral short hairpin RNA. We showed that histone deacetylases play critical roles in chromatin condensation and enucleation in cultured mouse fetal liver erythroblasts. Enzymatic inhibition of histone deacetylases by trichostatin A or valproic acid prior to the start of enucleation blocked chromatin condensation, contractile actin ring formation and enucleation. We further demonstrated that histone deacetylases 1, 2, 3 and 5 are highly expressed in mouse fetal erythroblasts. Short hairpin RNA down-regulation of histone deacetylase 2, but not of the other histone deacetylases, phenotypically mimicked the effect of trichostatin A or valproic acid treatment, causing significant inhibition of chromatin condensation and enucleation. Importantly, knock-down of histone deacetylase 2 did not affect erythroblast proliferation, differentiation, or apoptosis. These results identify histone deacetylase 2 as an important regulator, mediating chromatin condensation and enucleation in the final stages of mammalian erythropoiesis.

  10. Second molar germectomy and third molar eruption. 11 cases of lower second molar enucleation.

    Science.gov (United States)

    Gaumond, G

    1985-01-01

    Eleven cases followed from enucleation of the unerupted lower second molars through third molar eruption. While results were generally good, three presented third molar malpositions requiring further treatment.

  11. Evaluation of the learning curve for transurethral plasmakinetic enucleation and resection of prostate using a mentor-based approach

    Directory of Open Access Journals (Sweden)

    Lang Feng

    Full Text Available ABSTRACT Objective To analyze the mentor-based learning curve of one single surgeon with transurethral plasmakinetic enucleation and resection of prostate (PKERP prospectively. Materials and Methods Ninety consecutive PKERP operations performed by one resident under the supervision of an experienced endourologist were studied. Operations were analyzed in cohorts of 10 cases to determine when a plateau was reached for the variables such as operation efficiency, enucleation efficiency and frequency of mentor advice (FMA. Patient demographic variables, perioperative data, complications and 12-month follow-up data were analyzed and compared with the results of a senior urologist. Results The mean operative efficiency and enucleation efficiency increased from a mean of 0.49±0.09g/min and 1.11±0.28g/min for the first 10 procedures to a mean of 0.63±0.08g/min and 1.62±0.36g/min for case numbers 31-40 (p=0.003 and p=0.002. The mean value of FMA decreased from a mean of 6.7±1.5 for the first 10 procedures to a mean of 2.8±1.2 for case numbers 31-40 (p<0.01. The senior urologist had a mean operative efficiency and enucleation efficiency equivalent to those of the senior resident after 40 cases. There was significant improvement in 3, 6 and 12 month’s parameter compared with preoperative values (p<0.001. Conclusions PKERP can be performed safely and efficiently even during the initial learning curve of the surgeon when closely mentored. Further well-designed trials with several surgeons are needed to confirm the results.

  12. A Celestial Assisted INS Initialization Method for Lunar Explorers

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    Jiancheng Fang

    2011-07-01

    Full Text Available The second and third phases of the Chinese Lunar Exploration Program (CLEP are planning to achieve Moon landing, surface exploration and automated sample return. In these missions, the inertial navigation system (INS and celestial navigation system (CNS are two indispensable autonomous navigation systems which can compensate for limitations in the ground based navigation system. The accurate initialization of the INS and the precise calibration of the CNS are needed in order to achieve high navigation accuracy. Neither the INS nor the CNS can solve the above problems using the ground controllers or by themselves on the lunar surface. However, since they are complementary to each other, these problems can be solved by combining them together. A new celestial assisted INS initialization method is presented, in which the initial position and attitude of the explorer as well as the inertial sensors’ biases are estimated by aiding the INS with celestial measurements. Furthermore, the systematic error of the CNS is also corrected by the help of INS measurements. Simulations show that the maximum error in position is 300 m and in attitude 40″, which demonstrates this method is a promising and attractive scheme for explorers on the lunar surface.

  13. A celestial assisted INS initialization method for lunar explorers.

    Science.gov (United States)

    Ning, Xiaolin; Wang, Longhua; Wu, Weiren; Fang, Jiancheng

    2011-01-01

    The second and third phases of the Chinese Lunar Exploration Program (CLEP) are planning to achieve Moon landing, surface exploration and automated sample return. In these missions, the inertial navigation system (INS) and celestial navigation system (CNS) are two indispensable autonomous navigation systems which can compensate for limitations in the ground based navigation system. The accurate initialization of the INS and the precise calibration of the CNS are needed in order to achieve high navigation accuracy. Neither the INS nor the CNS can solve the above problems using the ground controllers or by themselves on the lunar surface. However, since they are complementary to each other, these problems can be solved by combining them together. A new celestial assisted INS initialization method is presented, in which the initial position and attitude of the explorer as well as the inertial sensors' biases are estimated by aiding the INS with celestial measurements. Furthermore, the systematic error of the CNS is also corrected by the help of INS measurements. Simulations show that the maximum error in position is 300 m and in attitude 40″, which demonstrates this method is a promising and attractive scheme for explorers on the lunar surface.

  14. Solar oscillation time delay measurement assisted celestial navigation method

    Science.gov (United States)

    Ning, Xiaolin; Gui, Mingzhen; Zhang, Jie; Fang, Jiancheng; Liu, Gang

    2017-05-01

    Solar oscillation, which causes the sunlight intensity and spectrum frequency change, has been studied in great detail, both observationally and theoretically. In this paper, owing to the existence of solar oscillation, the time delay between the sunlight coming from the Sun directly and the sunlight reflected by the other celestial body such as the satellite of planet or asteroid can be obtained with two optical power meters. Because the solar oscillation time delay is determined by the relative positions of the spacecraft, reflective celestial body and the Sun, it can be adopted as the navigation measurement to estimate the spacecraft's position. The navigation accuracy of single solar oscillation time delay navigation system depends on the time delay measurement accuracy, and is influenced by the distance between spacecraft and reflective celestial body. In this paper, we combine it with the star angle measurement and propose a solar oscillation time delay measurement assisted celestial navigation method for deep space exploration. Since the measurement model of time delay is an implicit function, the Implicit Unscented Kalman Filter (IUKF) is applied. Simulations demonstrate the effectiveness and superiority of this method.

  15. Direct fixation of extraocular muscles to a silicone sphere: a cost-sensitive, low-risk enucleation procedure.

    Science.gov (United States)

    Wells, Timothy S; Harris, Gerald J

    2011-01-01

    To describe a simple, cost-sensitive enucleation technique and to evaluate it in the context of intended benefits and long-term outcomes of alternate methods. A retrospective record review of patients who underwent enucleation using the described technique at a single institution by 2 surgeons. Surgery involved direct fixation of extraocular muscles to a solid silicone sphere, using nonabsorbable braided sutures with knots tied beneath the muscle insertions. Outcome measures included implant-related complications. Seventy-five patients meeting inclusion criteria ranged in age from 3 to 94 years (mean, 54 years; median, 56 years). Surgical indications included a blind painful or disrupted eye in 56 cases; uveal melanoma in 15 cases; retinoblastoma in 2 cases; and endophthalmitis in 2 cases. Follow-up intervals ranged from 3 to 57 months (mean, 27 months; median, 22 months). Complications requiring surgical revision of the implant occurred in 2 of the 75 cases (one luxation; one exposure). There were no instances of chronic socket discharge or implant infection. Nonabsorbable-suture attachment of muscles to a solid silicone implant offers an inexpensive enucleation option, with minimal risk of implant migration, exposure, or infection. In light of widespread disaffection with pegging of porous implants, and with no motility advantage of unpegged porous over nonporous implants, consideration should be given to techniques that are equally effective, less costly, and perhaps more reliable.

  16. Surgical treatment of odontogenic keratocyst by enucleation

    Directory of Open Access Journals (Sweden)

    Mamta Singh

    2010-01-01

    Full Text Available Although odontogenic keratocysts (OKCs are benign, they are often locally destructive and tend to recur after conservative surgical treatment. They must therefore be distinguished from other cysts of the jaw. Keratocysts possess outpouchings and microscopic daughter cysts from which recurrences may arise. Histologic examination is essential for diagnosis since the appearances on roentgenograms and at operation usually do not reveal the true nature of the lesion. Since many non-dental surgeons and pathologists are unaware of OKCs, a case is presented in which surgical treatment was by original conservative method. Decompression causes a reduction in the cyst volume with new bone formation so that the structures impinged upon (e.g., teeth, nerves are completely free.

  17. THE METHOD OF DESIGNING ASSISTED ON COMPUTER OF THE

    Directory of Open Access Journals (Sweden)

    LUCA Cornelia

    2015-05-01

    Full Text Available To the base of the footwear soles designing, is the shoe last. The shoe lasts have irregular shapes, with various curves witch can’t be represented by a simple mathematic function. In order to design the footwear’s soles it’s necessary to take from the shoe last some base contours. These contours are obtained with high precision in a 3D CAD system. In the paper, it will be presented a method of designing of the soles for footwear, computer assisted. The copying process of the shoe last is done using the 3D digitizer. For digitizing, the shoe last spatial shape is positioned on the peripheral of data gathering, witch follows automatically the shoe last’s surface. The wire network obtained through digitizing is numerically interpolated with the interpolator functions in order to obtain the spatial numerical shape of the shoe last. The 3D designing of the sole will be realized on the numerical shape of the shoe last following the next steps: the manufacture of the sole’s surface, the lateral surface realization of the sole’s shape, obtaining the link surface between the lateral side and the planner one of the sole, of the sole’s margin, the sole’s designing contains the skid proof area. The main advantage of the designing method is the design precision, visualization in 3D space of the sole and the possibility to take the best decision viewing the acceptance of new sole’s pattern.

  18. Enucleation of cultured mouse fetal erythroblasts requires Rac GTPases and mDia2.

    Science.gov (United States)

    Ji, Peng; Jayapal, Senthil Raja; Lodish, Harvey F

    2008-03-01

    Mammalian erythroid cells undergo enucleation, an asymmetric cell division involving extrusion of a pycnotic nucleus enveloped by the plasma membrane. The mechanisms that power and regulate the enucleation process have remained obscure. Here, we show that deregulation of Rac GTPase during a late stage of erythropoiesis completely blocks enucleation of cultured mouse fetal erythroblasts without affecting their proliferation or differentiation. Formation of the contractile actin ring (CAR) on the plasma membrane of enucleating erythroblasts was disrupted by inhibition of Rac GTPases. Furthermore, we demonstrate that mDia2, a downstream effector of Rho GTPases and a formin protein required for nucleation of unbranched actin filaments, is also required for enucleation of mouse fetal erythroblasts. We show that Rac1 and Rac2 bind to mDia2 in a GTP-dependent manner and that downregulation of mDia2, but not mDia1, by small interfering RNA (siRNA) during the late stages of erythropoiesis blocked both CAR formation and erythroblast enucleation. Additionally, overexpression of a constitutively active mutant of mDia2 rescued the enucleation defects induced by the inhibition of Rac GTPases. These results reveal important roles for Rac GTPases and their effector mDia2 in enucleation of mammalian erythroblasts.

  19. Indications for evisceration and enucleation of the eye at Ilorin, Nigeria

    African Journals Online (AJOL)

    In the period, 1990-1999, 92 eyes that underwent evisceration / enucleation at the University of Ilorin Teaching Hospital, Ilorin, Nigeria were reviewed retrospectively. Males were mostly affected with a male / female ratio of 2.8:1. The most common procedure done was evisceration in 85.9% of the cases. Enucleation was ...

  20. Worldwide enucleation techniques and materials for treatment of retinoblastoma: an international survey

    NARCIS (Netherlands)

    Mourits, Daphne L.; Hartong, Dyonne T.; Bosscha, Machteld I.; Kloos, Roel J. H. M.; Moll, Annette C.

    2015-01-01

    To investigate the current practice of enucleation with or without orbital implant for retinoblastoma in countries across the world. A digital survey identifying operation techniques and material used for orbital implants after enucleation in patients with retinoblastoma. We received a response of

  1. Indications for Enucleation of the Eyes of Children in Eastern Nigeria ...

    African Journals Online (AJOL)

    Retinoblastoma accounted for 36 (56.1%) enucleations, both sex being equally affected. Avoidable ocular problems resulting in enucleations such as panophthalmitis, endopphthalmitis, some staphyloma and others were noted in as high as 42.7% Trauma, measles and corneal ulcers of unknown aetiology preceded most ...

  2. Visual system plasticity in mammals: the story of monocular enucleation-induced vision loss

    Science.gov (United States)

    Nys, Julie; Scheyltjens, Isabelle; Arckens, Lutgarde

    2015-01-01

    The groundbreaking work of Hubel and Wiesel in the 1960’s on ocular dominance plasticity instigated many studies of the visual system of mammals, enriching our understanding of how the development of its structure and function depends on high quality visual input through both eyes. These studies have mainly employed lid suturing, dark rearing and eye patching applied to different species to reduce or impair visual input, and have created extensive knowledge on binocular vision. However, not all aspects and types of plasticity in the visual cortex have been covered in full detail. In that regard, a more drastic deprivation method like enucleation, leading to complete vision loss appears useful as it has more widespread effects on the afferent visual pathway and even on non-visual brain regions. One-eyed vision due to monocular enucleation (ME) profoundly affects the contralateral retinorecipient subcortical and cortical structures thereby creating a powerful means to investigate cortical plasticity phenomena in which binocular competition has no vote.In this review, we will present current knowledge about the specific application of ME as an experimental tool to study visual and cross-modal brain plasticity and compare early postnatal stages up into adulthood. The structural and physiological consequences of this type of extensive sensory loss as documented and studied in several animal species and human patients will be discussed. We will summarize how ME studies have been instrumental to our current understanding of the differentiation of sensory systems and how the structure and function of cortical circuits in mammals are shaped in response to such an extensive alteration in experience. In conclusion, we will highlight future perspectives and the clinical relevance of adding ME to the list of more longstanding deprivation models in visual system research. PMID:25972788

  3. Ultrastructure of basal vitreo-retina interface in enucleated pig eyes

    Directory of Open Access Journals (Sweden)

    Yi-Bin Xiong

    2013-04-01

    Full Text Available AIM: To investigate the ultrastructure of the basal vitreo- retina interface in enucleated pig eyes after vitreous injection of enzymes. METHODS:Totally 130 eyes were obtained from freshly slaughter pigs and divided into 5 groups. Each group was sub-divided into the 15 minutes group and the 30 minutes group, with 13 eyes in each group. The control group were performed middle vitreous injection of 0.1mL PBS, 200U/mL and 800U/mL hyaluronidase(HAwere injected in groups A and B, 10U/mL and 50U/mL chondroitinase(CAin groups C and D. After incubation for 15 minutes and 30 minutes, the eye balls were fixed with 4% glutaraldehyde and retina fixative solution. Pathological examination, eosin-hematoxylin staining, scan electron microscopy were taken to evaluate the remaining vitreous on vitreous base, transmission electron microscopy to evaluate retina toxicity. RESULTS:Both of gross appearance and hematoxylin+eosine slice all revealed basal vitreous partially liquefied and degradation. Remarkably decrease was found in basal vitreous than the control group both in the HA 800U/mL group and the CA 50U/mL group when using electron microscopy scanning. Transmission electron microscopy in group B, C and D revealed the remaining vitreous were less than the control group. CONCLUSION: Both CA and HA can cause basal vitreoretinal detachment in enucleated pig eyes. But the HA may cause less damage to the retina.

  4. The eyeball killer: serial killings with postmortem globe enucleation.

    Science.gov (United States)

    Coyle, Julie; Ross, Karen F; Barnard, Jeffrey J; Peacock, Elizabeth; Linch, Charles A; Prahlow, Joseph A

    2015-05-01

    Although serial killings are relatively rare, they can be the cause of a great deal of anxiety while the killer remains at-large. Despite the fact that the motivations for serial killings are typically quite complex, the psychological analysis of a serial killer can provide valuable insight into how and why certain individuals become serial killers. Such knowledge may be instrumental in preventing future serial killings or in solving ongoing cases. In certain serial killings, the various incidents have a variety of similar features. Identification of similarities between separate homicidal incidents is necessary to recognize that a serial killer may be actively killing. In this report, the authors present a group of serial killings involving three prostitutes who were shot to death over a 3-month period. Scene and autopsy findings, including the unusual finding of postmortem enucleation of the eyes, led investigators to recognize the serial nature of the homicides. © 2015 American Academy of Forensic Sciences.

  5. [FIRST CASES OF ANTEROPOSTERIOR DISSECTION TUEB (TRANSURETHRAL ENUCLEATION WITH BIPOLAR)].

    Science.gov (United States)

    Hosokawa, Shingo; Matsumoto, Rikiya; Mizuno, Takuji

    2015-01-01

    We examined the efficacy of anteroposterior dissection transurethral enucleation with bipolar (TUEB) for benign prostate hyperplasia (BPH). We performed anteroposterior dissection TUEB on 21 patients with BPH between October 2012 and June 2013. In anteroposterior dissection TUEB, the lateral lobes are anteroposteriorly dissected from the 12 o'clock position to the 6 o'clock position after enucleation of the middle lobe. Mean age was 73 years, mean estimated prostate volume was 62.8 ml, mean estimated transition zone volume was 44.5 ml, mean International Prostate Symptom Score (IPSS) score was 15.5, mean quality of life (QOL) score was 4.1, peak flow rate (Qmax) was 8.6 ml/s, and mean residual urine volume was 42.4 ml. The mean operation time was 69 min, the mean weight of removed prostate tissue was 27.3 g, and the mean decrease in hemoglobin level was 1.8 g/dl. Three months after surgery, mean IPSS score was 5.9, mean QOL score was 1.9, mean Qmax was 28 ml/s, and mean residual urine volume was 1.0 ml, values which were significantly improved over pre-surgery data. No complications such as perforation or bladder injury occurred during surgery, but 1 case (4.7%) of stress urinary incontinence and 1 case (4.7%) of urethral stricture were reported after surgery. Of note, urethral stricture was determined using a flexible cytoscope at 1 week, 1 month, and 3 months post-surgery. Anteroposterior dissection TUEB can be conducted safely and may be useful in reducing frequency of urethral stricture post-surgery.

  6. Clinicopathologic correlations in eyes enucleated after uveal melanoma resection with positive surgical margins.

    Science.gov (United States)

    Khalifa, Yousuf; Aaberg, Thomas M; Aaberg, Thomas M; Grossniklaus, Hans E

    2007-01-01

    We identified three eyes that had undergone enucleation after transscleral resection of uveal melanoma. Two enucleated eyes with microscopically positive margins of resection exhibited no evidence of residual melanoma and these patients were alive without metastasis with at least four years' follow-up. One eye with a transected melanoma contained residual melanoma and that patient died with metastatic melanoma to the liver three years after enucleation. There appear to be at least two general types of positive surgical margins of resection of uveal melanoma: microscopically positive margins and macroscopically positive (transected) margins of resection.

  7. Clinicopathologic correlations in eyes enucleated after uveal melanoma resection with positive surgical margins

    Directory of Open Access Journals (Sweden)

    Khalifa Yousuf

    2007-01-01

    Full Text Available We identified three eyes that had undergone enucleation after transscleral resection of uveal melanoma. Two enucleated eyes with microscopically positive margins of resection exhibited no evidence of residual melanoma and these patients were alive without metastasis with at least four years′ follow-up. One eye with a transected melanoma contained residual melanoma and that patient died with metastatic melanoma to the liver three years after enucleation. There appear to be at least two general types of positive surgical margins of resection of uveal melanoma: microscopically positive margins and macroscopically positive (transected margins of resection.

  8. CONSIDERING PEER ASSISTED LEARNING METHODS IN NEW ZEALAND’S SPECIAL NEEDS EDUCATION SECTOR

    Directory of Open Access Journals (Sweden)

    Donna IYER

    2011-09-01

    Full Text Available With the cuts to the public spending in the education sector extending to even students with special needs, the early childhood education sector and the public school system in New Zealand are under significant financial pressure. Elsewhere in the world, peer assisted learning methods have been identified as cost effective instruments with proven efficacy in improving the educational and social outcomes of children with disabilities. In New Zealand, peer assisted learning methods have been relatively slow on the uptake. The aim of this paper is to suggest peer assisted learning methods that can be potentially considered and utilised by the New Zealand’s early childhood education sector to support students with special needs. Based on a review of the international literature, peer tutoring and cross-age tutoring methods appear viable options. It is observed that peer assisted learning methods can complement conventional teaching methods.

  9. Giant Hepatic Hemangioma With Kasabach–Merritt Syndrome: Is the Appropriate Treatment Enucleation or Liver Transplantation?

    Science.gov (United States)

    Hochwald, Steven N.

    2000-01-01

    We present a case of giant cavernous hemangioma of the liver with disseminated intravascular coagulopathy (Kasabach–Merritt syndrome) which was cured by enucleation. The 51 year old woman presented with increased abdominal girth and easy bruisability. Workup elsewhere revealed a massive hepatic hemangioma and she was started on radiation therapy to the lesion and offered an orthotopic liver transplant. After careful preoperative preparation, we felt that resection was possible and she underwent a successful enucleation. The operation and postoperative course were complicated by bleeding but she recovered and remains well in followup after 6 months. All coagulation parameters have returned to normal. Enucleation should be considered the treatment of choice for hepatic hemangiomas, including those presenting with Kasabach–Merritt syndrome. The benefits of enucleation as compared to liver transplantation for these lesions are discussed. PMID:10977121

  10. Pancreatic enucleation using the da Vinci robotic surgical system: a report of 26 cases.

    Science.gov (United States)

    Shi, Yusheng; Peng, Chenghong; Shen, Baiyong; Deng, Xiaxing; Jin, Jiabin; Wu, Zhichong; Zhan, Qian; Li, Hongwei

    2016-12-01

    As a tissue-sparing procedure, pancreatic enucleation has become an alternative for benign or borderline pancreatic tumours; it has been proved to be safe and feasible. To date, a large sample size of robotic pancreatic enucleation has not been reported. This study aimed to discuss the clinical evaluation and postoperative complications after robotic pancreatic enucleation and compare it with open surgery. Patients who underwent robotic or open pancreatic enucleation during December 2010-December 2014 at Shanghai Ruijin Hospital, affiliated with the Shanghai Jiaotong University School of Medicine in China, were included. Clinical data were collected and analysed. Patients were divided into an open group and a robotic group: 26 patients underwent robotic pancreatic enucleation, of whom 13 patients were female. The mean age was 51.7 years, the operation time was 125.7 ± 58.8 min, blood loss was 49.4 ± 33.4 ml and mean tumour size was 18.8 ± 7.9 mm; 17 patients underwent open pancreatic enucleation, of whom 11 were female. The mean age was 54.6 ± 17.2 min, blood loss was 198.5 ± 70.7 ml and mean tumour size was 3.5 ± 1.9 cm. Pathology included insulinomas, intrapancreatic mucinous neoplasmas (IPMNs), pancreatic neuro-endocrine tumours (PNETs), solid pseudopapillary tumours (SPTs) and serous cystadenomas (SCAs). Robotic pancreatic enucleations were associated with less trauma, shorter operation time, less blood loss and faster wound recovery compared with open pancreatic enucleation. Pancreatic fistulas (PFs) were the main complication that occurred in the robotic group; infection also occurred in the open group. All patients recovered after effective drainage and the use of somatostatin. The mean follow-up time was 25 months. No recurrence was discovered, and one patient in the open group suffered endocrine insufficiency. Robotic pancreatic enucleation is a safe and effective surgical procedure for pancreatic benign and borderline tumours. It produces less

  11. ENUCLEATION IN A PATIENT WITH NEUROFIBROMATOSIS TYPE 1 WITH BUPHTHALMOS AND OCULAR DEFORMITY

    Directory of Open Access Journals (Sweden)

    F. Asadi-Amoli

    2007-09-01

    Full Text Available To report a case of buphthalmos with neurofibromatosis (NF-1 who suffered with blindness suffered enucleation. Physical examination & histopathology were performed. Physical examination revealed Buphthalmos & vascularized cornea with lower lid ectropion and orbital deformity. Histopathologic findings shows plexiform neurofibroma in orbital tissue with scleral wall involvement. And hamartomatous proliferation in choroid & cilliary bodies with melanocytic elements. Few NF-1 cases with buphthalmos have been reported. But cases with complicationg buphthalmos (retinal Detachment & optic nerve Atrophy suffering enucleation are very rare.

  12. [Laser enucleation of the prostate (HOLEP and THULEP): a comparative effectiveness analysis in treating recurrent prostatic hyperplasia].

    Science.gov (United States)

    Enikeev, D V; Glybochko, P V; Alyaev, Yu G; Rapoport, L M; Sorokin, N I; Enikeev, M E; Dymov, A M; Sukhanov, R B; Khamraev, O Kh; Taratkin, M S; Dymova, A V; Inoyatov, J S

    2017-09-01

    enucleation of the prostate is not only an effective treatment for infravesical obstruction due to benign prostatic hyperplasia, but is also a method of choice in the treatment of patients with recurrent BPH.

  13. Optimization of ultrasound assisted extraction of anthocyanins from red cabbage using Taguchi design method

    National Research Council Canada - National Science Library

    Ravanfar, Raheleh; Tamadon, Ali Mohammad; Niakousari, Mehrdad

    2015-01-01

    ... (output power, time, temperature and pulse mode) for ultrasound assisted extraction of anthocyanins from red cabbage and the concluding yield of anthocyanin was measured by pH differential method...

  14. New insights into the mechanisms of mammalian erythroid chromatin condensation and enucleation.

    Science.gov (United States)

    Ji, Peng

    2015-01-01

    A unique feature in mammalian erythropoiesis is the dramatic chromatin condensation followed by enucleation. This step-by-step process starts at the beginning of terminal erythropoiesis after the hematopoietic stem cells are committed to erythroid lineage. Although this phenomenon is known for decades, the mechanisms of chromatin condensation and enucleation remain elusive. Recent advances in cell and molecular biology have started to reveal the molecular pathways in the regulation of chromatin condensation, the establishment of nuclear polarity prior enucleation, and the rearrangement of actin cytoskeleton in enucleation. However, many challenging questions, especially whether and how the apoptotic mechanisms are involved in chromatin condensation and how to dissect the functions of many actin cytoskeleton proteins in cytokinesis and enucleation, remain to be answered. Here I review our current understanding of mammalian erythroid chromatin condensation and enucleation during terminal differentiation with a focus on more recent studies. I conclude with my perspective of future works in this rising topic in developmental and cell biology. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Enucleation in Iceland 1992-2004: study in a defined population.

    Science.gov (United States)

    Geirsdottir, Asbjorg; Agnarsson, Bjarni A; Helgadottir, Gudleif; Sigurdsson, Haraldur

    2014-03-01

    To determine the incidence rate as well as causative diagnoses and surgical indications of enucleation in Iceland during the years 1992-2004. A retrospective population-based incidence study involving the entire population of Iceland. Medical records of all patients who underwent enucleation in Iceland from January 1992 through December 2004 were reviewed. The annually updated Icelandic census was used as a denominator data. Fifty-six eyes were enucleated during 1992-2004. No eviscerations were done, and the three exenterations performed were not included in the study. The mean annual age-adjusted incidence rate of enucleation in Iceland was 1.48 enucleations per 100 000 population in comparison with 2.66 enucleations per 100 000 for the time period 1964-1991. With advancing age, a significant increasing linear trend existed (p Iceland is continually decreasing, although the incidence of severe ocular trauma and ocular malignancy is fairly stable. © 2012 The Authors. Acta Ophthalmologica © 2012 Acta Ophthalmologica Scandinavica Foundation.

  16. Radiological changes post-lymph node enucleation for airway obstruction in children with pulmonary tuberculosis.

    Science.gov (United States)

    Maydell, Arthur; Goussard, Pierre; Andronikou, Savvas; Bezuidenhout, Fourie; Ackermann, Christelle; Gie, Robert

    2010-10-01

    Tuberculous lymphadenopathy causing airway obstruction in children may be life threatening and may require surgical enucleation of the lymph glands. There are no studies investigating the radiological picture post-enucleation. We attempt to explore this area in our study. A retrospective study of the imaging in 21 paediatric cases having undergone tuberculosis (TB) lymph node enucleation. Bronchus intermedius (BI) stenosis was present in 95% of patients undergoing enucleation, followed by left main bronchus (LMB) (81%) and right main bronchus (RMB) (67%) stenosis. Right lung collapse/consolidation occurred more frequently (48-62%) than left-lung collapse/consolidation (10-14%). Resolution of BI stenosis and right lower lobe (RLL) collapse/consolidation is the most consistent postoperative finding. Nine children resolved at an average time of 6.5 months postoperatively, while 10 children were still resolving at an average time of 4.5 months. The resolution of the complications of lymph node enlargement (airway stenosis and lung collapse/consolidation) was seen more frequently than the resolution of the offending lymphadenopathy itself. Right-sided disease was necessary to produce complications severe enough to require enucleation. Subcarinal lymph node enucleation is sufficient for resolution of LMB stenosis and associated left-lung sequelae. Copyright © 2010 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved.

  17. Towards optimizing prostate tissue retrieval following holmium laser enucleation of the prostate (HoLEP): Assessment of two morcellators and review of literature.

    Science.gov (United States)

    Elshal, Ahmed M; Mekkawy, Ramy; Laymon, Mahmoud; El-Assmy, Ahmed; El-Nahas, Ahmed R

    2015-01-01

    We assess different approaches to retrieve the enucleated adenoma after transurethral enucleation of the prostate, particularly using the holmium laser. A retrospective review through our prospectively maintained database was performed looking for safety and efficacy of two morcellators. The enucleation phase of the holmium laser enucleation of the prostate (HoLEP) was classically performed followed by retrieval of the intravesical adenoma using either the Piranha (Wolf Inc., Knittlingen, Germany) or VersaCut (Lumenis) morcellator. A PubMed-MEDLINE search was conducted for all transurethral enucleation procedures and relevant data regarding methods of prostate tissue retrieval were extracted. Strictly limiting the study to 3 reusable blades with each morcellator, we performed 67 and 55 consecutive procedures with Piranha and VersaCut, respectively. There was no significant difference between the two morcellators regarding perioperative complications, apart from 5 bladder mucosal injuries with the VersaCut (9%). Furthermore, there were similar retrieved tissue weight, mechanical problems-rate, catheter-time and hospital-stay in both morcellators. However, the Piranha morcellator needed significantly less morcellation-time, needed to use cold loop to remove non-morcellated pieces and to score the adenoma by laser for better bite of the adenoma, and had a higher median morcellation-rate 6.2 (rate: 2.8-12) g/min. Despite little reporting on morcellation, we had data on the tissue retrieval rate (2.6 to 6.5 g/min with Piranha and 1.9 to 11 g/min with VersaCut. Furthermore, bladder mucosal injury was reported in 1.4% and 0.7 to 5.7% with Piranha and VersaCut, respectively; bladder perforation with VersaCut was experienced in about 0.1 to 1.5% of patients. Our study is limited by its non-randomization. The Piranha morcellator was the most efficient and safe way to retrieve tissue after a transurethral enucleation of a prostate adenoma.

  18. Enucleation: A treatment alternative for branch duct intraductal papillary mucinous neoplasms.

    Science.gov (United States)

    Kaiser, Joerg; Fritz, Stefan; Klauss, Miriam; Bergmann, Frank; Hinz, Ulf; Strobel, Oliver; Schneider, Lutz; Büchler, Markus W; Hackert, Thilo

    2017-03-01

    Small, asymptomatic, branch-duct intraductal papillary mucinous neoplasms of the pancreas are often kept under surveillance despite their malignant potential. The management of branch-duct intraductal papillary mucinous neoplasm is controversial with regard to indications and extent of any operative intervention. The present study aimed to evaluate enucleation as an alternative operative approach for branch-duct intraductal papillary mucinous neoplasms to exclude and prevent malignancy. For branch-duct intraductal papillary mucinous neoplasms of neoplasm on the basis of these features between January 2004 and September 2014 were analyzed. Among these, patients with successful enucleation were compared with those who were scheduled for enucleation but converted intraoperatively to pancreatic resection (intention-to-treat analysis). End points were hospital morbidity and mortality as well as histopathology and functional outcome at a mean follow-up of 32 months. In the study, 115 patients with presumed branch-duct intraductal papillary mucinous neoplasm and the intention to perform pancreatic enucleation were included; 87 enucleations were performed in 74 patients. In 41 patients, enucleation was converted to a pancreatic resection (procedure-specific success rate 64%); indications for conversion included location or size (46%), presence of multicystic lesions (39%), or involvement of the main pancreatic duct (15%). Of the 74 patients with enucleation, 64 branch-duct intraductal papillary mucinous neoplasms revealed low- (85%), 11% moderate dysplasia-, and 4% high-grade dysplasia on histology. Among converted resections, 6 intraductal papillary mucinous neoplasms revealed high-grade dysplasia or invasive carcinoma (15%). Intention-to-treat analysis with patients converted to pancreatic resection showed that enucleations resulted in less blood loss (100 vs 400 mL) and a shorter operation time (146 vs 255 minutes; P neoplasm-specific recurrence rates (3% vs 6

  19. Translation Method and Computer Programme for Assisting the Same

    DEFF Research Database (Denmark)

    2013-01-01

    , a machine translation system translating the written source text into a set of translations hypotheses in the target language, and an integration module combining the set of spoken word hypotheses and the set of machine translation hypotheses obtaining a text in the target language. Thereby obtaining......The present invention relates to a translation method comprising the steps of: a translator speaking a translation of a written source text in a target language, an automatic speech recognition system converting the spoken translation into a set of phone and word hypotheses in the target language...... a significantly improved and faster translation compared to what is achieved by known translation methods....

  20. Method and apparatus for jet-assisted drilling or cutting

    Science.gov (United States)

    Summers, David Archibold; Woelk, Klaus Hubert; Oglesby, Kenneth Doyle; Galecki, Grzegorz

    2013-07-02

    An abrasive cutting or drilling system, apparatus and method, which includes an upstream supercritical fluid and/or liquid carrier fluid, abrasive particles, a nozzle and a gaseous or low-density supercritical fluid exhaust abrasive stream. The nozzle includes a throat section and, optionally, a converging inlet section, a divergent discharge section, and a feed section.

  1. Peer-Assisted Learning in Research Methods and Statistics

    Science.gov (United States)

    Stone, Anna; Meade, Claire; Watling, Rosamond

    2012-01-01

    Feedback from students on a Level 1 Research Methods and Statistics module, studied as a core part of a BSc Psychology programme, highlighted demand for additional tutorials to help them to understand basic concepts. Students in their final year of study commonly request work experience to enhance their employability. All students on the Level 1…

  2. Method and apparatus for jet-assisted drilling or cutting

    Science.gov (United States)

    Summers, David Archibold; Woelk, Klaus Hubert; Oglesby, Kenneth Doyle; Galecki, Grzegorz

    2012-09-04

    An abrasive cutting or drilling system, apparatus and method, which includes an upstream supercritical fluid and/or liquid carrier fluid, abrasive particles, a nozzle and a gaseous or low-density supercritical fluid exhaust abrasive stream. The nozzle includes a throat section and, optionally, a converging inlet section, a divergent discharge section, and a feed section.

  3. New mobile methods for dietary assessment: review of image-assisted and image-based dietary assessment methods.

    Science.gov (United States)

    Boushey, C J; Spoden, M; Zhu, F M; Delp, E J; Kerr, D A

    2017-08-01

    For nutrition practitioners and researchers, assessing dietary intake of children and adults with a high level of accuracy continues to be a challenge. Developments in mobile technologies have created a role for images in the assessment of dietary intake. The objective of this review was to examine peer-reviewed published papers covering development, evaluation and/or validation of image-assisted or image-based dietary assessment methods from December 2013 to January 2016. Images taken with handheld devices or wearable cameras have been used to assist traditional dietary assessment methods for portion size estimations made by dietitians (image-assisted methods). Image-assisted approaches can supplement either dietary records or 24-h dietary recalls. In recent years, image-based approaches integrating application technology for mobile devices have been developed (image-based methods). Image-based approaches aim at capturing all eating occasions by images as the primary record of dietary intake, and therefore follow the methodology of food records. The present paper reviews several image-assisted and image-based methods, their benefits and challenges; followed by details on an image-based mobile food record. Mobile technology offers a wide range of feasible options for dietary assessment, which are easier to incorporate into daily routines. The presented studies illustrate that image-assisted methods can improve the accuracy of conventional dietary assessment methods by adding eating occasion detail via pictures captured by an individual (dynamic images). All of the studies reduced underreporting with the help of images compared with results with traditional assessment methods. Studies with larger sample sizes are needed to better delineate attributes with regards to age of user, degree of error and cost.

  4. Thulium laser enucleation of the prostate (TmLEP) vs. transurethral resection of the prostate (TURP): evaluation of early results.

    Science.gov (United States)

    Świniarski, Piotr Paweł; Stępień, Stanisław; Dudzic, Waldemar; Kęsy, Stanisław; Blewniewski, Mariusz; Różański, Waldemar

    2012-01-01

    The first decade of XXI century it is a time of the thulium laser implementation to a benign prostatic hyperplasia treatment. The objective of this paper is a comparative assessment of early results thulium laser enucleation of the prostate (TmLEP) versus transurethral resection of the prostate (TURP) in 3-months observation. PATIENTS WERE RANDOMIZED TO BPH SURGICAL TREATMENT: research group (TmLEP - 54 men) or control group (TURP - 52 men). Between 02.2007-09.2009 non-consecutive patients were examined before, one month, and 3-months after surgery. Perioperative data (age, PV, time of surgery, use of laser, morcellation, catheterization, hospitalization, used energy, Hgb loss and removed tissue weight) were assessed. Before and after surgery IPSS, QoL, Qmax and PVR were controlled. Hemoglobin loss was twice lower during TmLEP than TURP [0.95 ±0.77 (0-3.2) vs. 1.81 ±0.97 (0.1-4.7) g/dl, p enucleation of the prostate is safe and efficient BPH treatment method, comparable to the transurethral electroresection in 3-months observation. Lack of long-term research does not allow to form wider conclusions.

  5. A new method for optimization of low-thrust gravity-assist sequences

    Science.gov (United States)

    Maiwald, V.

    2017-09-01

    Recently missions like Hayabusa and Dawn have shown the relevance and benefits of low-thrust spacecraft concerning the exploration of our solar system. In general, the efficiency of low-thrust propulsion is one means of improving mission payload mass. At the same time, gravity-assist maneuvers can serve as mission enablers, as they have the capability to provide "free energy." A combination of both, gravity-assist and low-thrust propulsion, has the potential to generally improve mission performance, i.e. planning and optimization of gravity-assist sequences for low-thrust missions is a desirable asset. Currently no established methods exist to include the gravity-assist partners as optimization variable for low-thrust missions. The present paper explains how gravity-assists are planned and optimized, including the gravity-assist partners, for high-thrust missions and discusses the possibility to transfer the established method, based on the Tisserand Criterion, to low-thrust missions. It is shown how the Tisserand Criterion needs to be adapted using a correction term for the low-thrust situation. It is explained why this necessary correction term excludes an a priori evaluation of sequences and therefore their planning and an alternate approach is proposed. Preliminary results of this method, by application of a Differential Evolution optimization algorithm, are presented and discussed, showing that the method is valid but can be improved. Two constraints on the search space are briefly presented for that aim.

  6. Transurethral enucleation and resection of prostate in patients with benign prostatic hyperplasia by plasma kinetics.

    Science.gov (United States)

    Liu, Chunxiao; Zheng, Shaobo; Li, Hulin; Xu, Kai

    2010-12-01

    We evaluated transurethral enucleation and resection of the prostate in patients with urinary symptoms due to benign prostatic hyperplasia using the Plasmakinetic™ system. We retrospectively analyzed the records of 1,100 patients who underwent transurethral enucleation and resection of the prostate between January 2003 and February 2009 at our institution. We assessed the International Prostate Symptom Score, quality of life score, peak flow rate and post-void residual urine volume preoperatively, 1, 3, 6 and 12 months postoperatively, and yearly thereafter. Enucleation and resection time, enucleated tissue weight, catheterization time, hospital stay and long-term complications were recorded. No patient had significant blood loss or signs of the transurethral resection syndrome. Mean±SD patient age was 66.7±7.3 years and mean followup was 4.3 years. Mean preoperative prostate weight was 67.7±12 gm (range 35 to 256), mean enucleation time was 15.5 minutes (range 10 to 38), mean resection time was 46 minutes (range 20 to 65) and mean resected tissue weight was 42.8±7.7 gm (range 23 to 219). Mean catheter time was 1.8±0.4 days and mean hospital stay was 5.3±2.3 days. Transurethral enucleation and resection of the prostate induced significant, pronounced, immediate and lasting improvement in the International Prostate Symptom Score, quality of life, maximum urinary flow and post-void residual urine volume. Postoperative complications included meatal stenosis in 9 cases, incontinence in 56, urethral stricture in 12 and bladder neck contracture in 10. Transurethral enucleation and resection of the prostate appears to be the modern alternative to transurethral resection of the prostate and open prostatectomy for bladder outlet obstruction due to benign prostatic hyperplasia. It may be done in glands up to 250 gm. Copyright © 2010 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  7. Outcomes of Holmium Laser Enucleation of the Prostate in the Re-Treatment Setting.

    Science.gov (United States)

    Marien, Tracy; Kadihasanoglu, Mustafa; Tangpaitoon, Teerayut; York, Nadya; Blackburne, Andrew T; Abdul-Muhsin, Haidar; Borofsky, Michael S; Krambeck, Amy E; Humphreys, Mitchell R; Lingeman, James E; Miller, Nicole L

    2017-06-01

    Holmium laser enucleation of the prostate can also be applied in the re-treatment setting when other benign prostatic hyperplasia therapies fail. We compared outcomes in men who underwent holmium laser enucleation of the prostate in the primary vs the re-treatment setting. We retrospectively reviewed the records of 2,242 patients who underwent holmium laser enucleation of the prostate at a total of 4 academic hospitals between 2003 and 2015. Patient demographics, and operative and perioperative outcomes were compared between re-treatment and primary holmium laser enucleation of the prostate. Of the 360 of 2,242 men (16%) who underwent re-treatment holmium laser enucleation of the prostate the procedure was done for residual urinary symptoms in 71%. The most common primary procedure was transurethral resection of the prostate in 42% of cases. Mean time between prior benign prostatic hyperplasia surgery and re-treatment was 68 months (range 1 to 444). There were no significant differences in age, prostate size, AUA (American Urological Association) symptom score or average flow rate between the cohorts. Perioperatively, re-treatment holmium laser enucleation of the prostate was associated with significantly shorter operative time, reduced blood loss, lower specimen weight and shorter length of stay. The AUA symptom score improved in both groups, although it remained higher in men who underwent re-treatment (6.5 vs 5.0, p enucleation of the prostate performed in the re-treatment setting were no different from those in the primary setting. While re-treatment was associated with an increased likelihood of clot retention, urethral stricture and higher AUA symptom score, these minimal differences must be considered against the overall favorable symptom improvement across both cohorts. Copyright © 2017 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  8. Holmium Laser Enucleation of the Prostate (HoLEP: Our Experience with the Learning Curve and the Development of the ‘En-Bloc No-Touch’ Technique

    Directory of Open Access Journals (Sweden)

    Cesare Marco Scoffone

    2015-05-01

    Full Text Available Background: Holmium laser enucleation of the prostate (HoLEP is a safe and effective therapeutic option in patients suffering from benign prostatic hyperplasia (BPH of any size. In spite of its excellent and durable outcomes, HoLEP is gaining widespread acceptance very slowly, since it is perceived as requiring significant endoscopic skill and having a steep learning curve. Here we present our 4-year experience with this technique after more than 200 cases, describing our learning curve with the traditional three-lobe technique of Gilling, and its progressive modification into the so-called ‘en-bloc no-touch’ technique. Methods: From January 2011 to December 2014, 200 consecutive patients diagnosed with symptomatic and obstructive BPH underwent HoLEP in our department. Demographic and clinical data were prospectively collected. Age, total operating time, enucleation time and efficiency, morcellation time, energy employed, adenoma weight, hospital stay, and complications were recorded. Results: The HoLEP learning curve in our department included an initial 1-year experience with the traditional technique of Gilling, and its progressive modification with the development of the socalled ‘en-bloc no-touch’ approach, subsequently standardised step by step. At the beginning of the learning curve short time intervals between the procedures are relevant for faster learning. With time and experience, adenomas of all sizes are treated, with significantly shorter total operating and enucleation times, significantly increased enucleation efficiency, decreased use of energy (meaning fewer postoperative voiding symptoms, and fewer complications. Morcellation time is more devicedependent than surgeon-dependent, and is also influenced by the composition of the adenomatous tissue. Conclusion: The ‘en-bloc no-touch’ technique seems to simplify the procedure, making it easier to teach and to learn. HoLEP safety and efficacy are improved by increasing

  9. Vision-Related Quality of Life and Appearance Concerns Are Associated with Anxiety and Depression after Eye Enucleation: A Cross-Sectional Study

    Science.gov (United States)

    Ye, Juan; Lou, Lixia; Jin, Kai; Xu, Yufeng; Ye, Xin; Moss, Timothy; McBain, Hayley

    2015-01-01

    Aims To investigate the association of demographic, clinical and psychosocial variables with levels of anxiety and depression in participants wearing an ocular prosthesis after eye enucleation. Methods This cross-sectional study included 195 participants with an enucleated eye who were attending an ophthalmic clinic for prosthetic rehabilitation between July and November 2014. Demographic and clinical data, and self-reported feelings of shame, sadness and anger were collected. Participants also completed the National Eye Institute Visual Function Questionnaire, the Facial Appearance subscale of the Negative Physical Self Scale, and the Hospital Anxiety and Depression Scale. Regression models were used to identify the factors associated with anxiety and depression. Results The proportion of participants with clinical anxiety was 11.8% and clinical depression 13.8%. More anxiety and depression were associated with poorer vision-related quality of life and greater levels of appearance concerns. Younger age was related to greater levels of anxiety. Less educated participants and those feeling more angry about losing an eye are more prone to experience depression. Clinical variables were unrelated to anxiety or depression. Conclusions Anxiety and depression are more prevalent in eye-enucleated patients than the general population, which brings up the issues of psychiatric support in these patients. Psychosocial rather than clinical characteristics were associated with anxiety and depression. Longitudinal studies need to be conducted to further elucidate the direction of causality before interventions to improve mood states are developed. PMID:26317860

  10. Vaporization vs. enucleation techniques for BPO: do we have a standard?

    Science.gov (United States)

    Netsch, Christopher; Bach, Thorsten

    2015-01-01

    This article discusses enucleation and vaporization procedures which have been developed on the surgical techniques of holmium laser enucleation of the prostate (HoLEP) and photoselective vaporization of the prostate (PVP) by reviewing the most recent publications. Enucleation procedures have been described using holmium, thulium, diode or GreenLight lasers in addition to bipolar energy sources. Most of the current literature for these enucleation procedures consists of initial descriptions of the surgical techniques or prospective series from single centres, although the availability of prospective randomized trial for these procedures other than HoLEP is limited. PVP have been described using 80-W, 120-W, or 180-W GreenLight lasers. To date, only sparse literature is available for thulium or bipolar vaporization of the prostate. A variety of alternative vaporization and enucleation procedures are available for transurethral treatment of benign prostatic obstruction. Only very few PRT have been published for these procedures limiting their evidence for the treatment of benign prostatic obstruction. To date, best evidence is still available for the HoLEP and PVP procedure.

  11. Transurethral holmium laser enucleation versus transurethral resection of the prostate and simple open prostatectomy--which procedure is faster?

    Science.gov (United States)

    Ahyai, Sascha A; Chun, Felix K H; Lehrich, Karin; Dahlem, Roland; Zacharias, Mario S; Fisch, Margit M; Kuntz, Rainer M

    2012-05-01

    The longer operative time of holmium laser enucleation of the prostate compared to transurethral resection of the prostate or simple open prostatectomy reported in the literature might have been biased by the unavailability of a soft tissue morcellator, limited surgical experience with holmium laser prostate enucleation or the fact that significantly more tissue was removed by enucleation than by resection. We objectively compared the resection speed of contemporary holmium laser enucleation vs transurethral resection of the prostate and of holmium laser enucleation vs simple open prostatectomy. The study cohort consisted of 100 cases of transurethral prostate resection and 60 of simple open prostatectomy from our previous randomized, controlled trials. These cases were subjected to matched pair analysis with greater than 1,000 from our prospective contemporary database on holmium laser prostate enucleation. Exact matches were made for the same amount of resected tissue. In all contemporary holmium laser enucleation cases a mechanical soft tissue morcellator was used. We calculated and compared the specific resection speed in gm per minute and operative time for the same amount of resected tissue. In groups 1 and 2 we matched 99 exact laser enucleation-transurethral resection pairs and 53 exact laser enucleation-simple open prostatectomy pairs, respectively. Resection speed and operative time for laser enucleation were statistically significantly faster than for resection (0.61 vs 0.51 gm per minute and 62 vs 73 minutes, p enucleation of the prostate is faster than transurethral resection of the prostate and similar to simple open prostatectomy. Copyright © 2012 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  12. A case of lipoma of lateral anterior neck treated with surgical enucleation

    Directory of Open Access Journals (Sweden)

    Francesco Grecchi

    2012-01-01

    Full Text Available Lipoma arise in almost 50% of all soft tumours. The neck lipomas are rare tumours that may present as painless masses with slow growth, in the lateral portions of the neck. Some lipomas, such as the one studied in our case, grow deep in the subcutaneous tissue, in close contact with muscles. Here, we report a case of lipoma extending from pre-tragal region up to the ascending branch of the mandible in a 62 year old man, treated with enucleation. The inferior margin of lipoma involved the pharyngeal and the superior margin was achieved by the top of the skull base. The mass of lipoma caused breathing difficulties in the patient, preventing regular sleep. No complication was recorded in the post-operative period and no further surgery was performed. The complete resolution after one year′s follow-up, together with the rarity of the anatomical site, makes this case worthy of description. A correct diagnosis facilitated removal of this lesion with a surgical method.

  13. Holmium Laser enucleation of the prostate (HoLEP versus Transurethral Resection of the Prostate (TURP

    Directory of Open Access Journals (Sweden)

    Luís Eduardo Durães Barboza

    Full Text Available OBJECTIVE:to evaluate the effectiveness and applicability of Holmium laser enucleation of the prostate (HoLEP - in the treatment of benign prostatic hyperplasia (BPH - in comparison to transurethral resection of the prostate (TURP.METHODS:patients with symptomatic prostatic hyperplasia and candidates for surgical treatment were selected. Both procedures were explained and they had choosen HoLEP or TURP. At the hospital were collected: age, date of birth, international prostate symptom score, urinary peak flow rate, prostate volume, post-voiding residual urine, globular volume and serum PSA. At the procedure operating time, morcellating time (HoLEP, bladder mucosal injury and intercurrences were collected. At the first postoperative day, globular volume and sodium. Besides that were observed the catheter indwelling time and hospital stay and after 90 days, urinary peak flow rate and international prostate symptom score. Statistical analisys have been done partially by Sinpe(r and also by a professional team.RESULTS:twenty patients in HoLEP group and 21 at TURP were operated. Baseline urinary peak flow rate was 8 ml/s in both groups and preoperative international prostate symptom score was 22 in HoLEP and 20 in TURP, very similar. Operative time was 85 minutes in HoLEP and 60 in TURP, pCONCLUSION:HoLEP is a feasible technique and is as effective as TURP on symptomatic prostatic hyperplasia surgical treatment.

  14. Comparison of fluid absorption between transurethral enucleation and transurethral resection for benign prostate hyperplasia.

    Science.gov (United States)

    Ran, Longfei; He, Weiyang; Zhu, Xin; Zhou, Qingsong; Gou, Xin

    2013-01-01

    Although transurethral resection of prostate (TURP) remains the reference standard for benign prostate hyperplasia (BPH), the concern about complications promotes researchers to develop alternative surgical methods with fewer complications. In this study, we compared the safety and efficacy between the transurethral plasma kinetic enucleation of prostate (TUPKEP) and transurethral plasma kinetic resection of prostate (TUPKRP), mainly including absorption of irrigation fluid, the operation time, the weight of prostate tissue removed and severe complications. Sixty BPH patients were randomly and evenly assigned to the TUPKEP or TUPKRP group. The irrigation fluid used in both groups was 1% ethanol-containing saline solution. The ethanol concentrations in the subjects' end expiration were measured during operation. The volume of irrigation fluid absorbed was calculated accordingly. No significant difference was found in operation time between two groups, whereas the weight of prostate tissue resection was significantly higher in the TUPKEP than that in the TUPKRP group. The study provides evidence for the safety, feasibility and effectiveness of both bipolar transurethral techniques. Further, compared to the TUPKRP group, the TUPKEP group has more efficient for resection of prostatic hyperplasia tissue, even though in terms of fluid absorption, no difference has been found in both groups. Ethanol monitoring is simple, safe and effective, which is beneficial for enhancing safety procedures. Copyright © 2013 S. Karger AG, Basel.

  15. Autogenous Partial Bone Chip Grafting on the Exposed Inferior Alveolar Nerve After Cystic Enucleation.

    Science.gov (United States)

    Seo, Mi Hyun; Eo, Mi Young; Cho, Yun Ju; Kim, Soung Min; Lee, Suk Keun

    2017-10-26

    This prospective study evaluated the clinical effectiveness of the new approach of partial autogenous bone chip grafts for the treatment of mandibular cystic lesions related to the inferior alveolar nerve (IAN). A total of 38 patients treated for mandibular cysts or benign tumors were included in this prospective study and subsequently divided into 3 groups depending on the bone grafting method used: cystic enucleation without a bone graft (group 1), partial bone chip graft covering the exposed IAN (group 2), and autogenous bone graft covering the entire defect (group 3). We evaluated the symptoms, clinical signs, and radiographic changes using dental panorama preoperatively, immediate postoperatively, and at 1, 3, 6, and 12 months postoperatively. Radiographic densities were compared using Adobe Photoshop CS5 (Adobe Systems Inc., San Jose, CA). Repeated measures analysis of variance was used for statistical evaluation with SPSS 22.0 (SPSS Inc, Chicago, IL), and P < 0.05 was considered statistically significant.Radiopacities were the most increased at 1 year postoperative in group 3; groups 2 and 3 did not show statistically significant differences, whereas groups 1 and 3 were statistically significant. In terms of radiographic bone healing with clinical regeneration of the exposed IAN, healing occurred in all patients, although the best healing was achieved in group 2.This autogenous partial bone chip grafting procedure to cover the exposed IAN is suggested as a new surgical protocol for the treatment of cystic lesions associated with the IAN.

  16. Safety and efficacy of Eraser laser enucleation of the prostate: preliminary report.

    Science.gov (United States)

    Lusuardi, Lukas; Myatt, Andrew; Sieberer, Manuela; Jeschke, Stefan; Zimmermann, Reinhold; Janetschek, Günter

    2011-11-01

    We ascertained the safety and efficacy of the 1,318 nm diode Eraser laser (Rolle and Rolle, Salzburg, Austria) for transurethral enucleation of the prostate. This laser has been successfully used to resect lung metastasis. It cuts and coagulates vascular rich tissue safely and effectively. We describe a prospective, randomized trial of Eraser laser prostate enucleation vs bipolar transurethral prostate resection. A total of 60 patients with lower urinary tract symptoms suggesting bladder outlet obstruction and a mean prostate size of 59.5 ml on transrectal ultrasound were randomized to Eraser laser prostate enucleation or bipolar transurethral prostate resection. Patients were assessed preoperatively, and 1 and 6 months postoperatively. Eraser laser prostate enucleation was equivalent to bipolar transurethral prostate resection in improvement in International Prostate Symptom Score, maximal flow rate and quality of life. Laser enucleation was significantly superior to bipolar transurethral resection for measured blood loss (mean ± SD 116.83 ± 97.02 vs 409.83 ± 148.61 ml), catheter time (mean 32.80 ± 8.74 vs 65.73 ± 13.72 hours) and hospital time (mean 45.13 ± 14.77 vs 91.20 ± 11.76 hours, each p enucleation and bipolar transurethral prostate resection were equally safe and effective to relieve bladder outflow obstruction and lower urinary tract symptoms. This laser technique has the advantage of less blood loss, and shorter catheter time and hospital stay. Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  17. Transurethral GreenLight laser enucleation of the prostate--a feasibility study.

    Science.gov (United States)

    Brunken, Claus; Seitz, Christian; Tauber, Stephan; Schmidt, Rainer

    2011-07-01

    GreenLight laser vaporization is established as a minimally invasive procedure to treat patients with benign prostatic hyperplasia. Despite good functional results, it may be difficult to achieve adequate tissue removal for large prostates. In this study, we evaluated whether a transurethral enucleation technique is feasible with the GreenLight laser as a possible way to improve the amount of tissue removed. Following the technique described by Gilling for the holmium laser, we carried out transurethral enucleation of prostate adenoma with the 120W HPS GreenLight laser in 21 consecutive patients. Preoperative data were collected prospectively; prostate volume, International Prostate Symptom Score (IPSS), postvoid residual (PVR), prostate-specific antigen level, peak urinary flow rate, operative time, catherization period, length of hospitalization, and perioperative complications were recorded as well as the weight of the enucleated tissue and the applied laser energy. For follow-up, IPSS and PVR were recorded. Data are presented as mean±standard deviation. Fifty-two percent of the patients had preoperative urinary retention. Preoperative prostate volume was 74.6±21.7 cc; 34.7±21.7 g of tissue were enucleated. IPSS was reduced from 25±6 to 5±9 (P=0.0001), PVR from 126±80 to 11±18 (P=0.002) by GreenLight laser enucleation. Serious complications were not observed. Operative time was 112±27 minutes. Catheter time was 1.2±0.4 days. The length of hospitalization was 3.6±0.9 days. GreenLight laser enucleation is feasible and safe. Tissue reduction is complete, and good functional results are achieved. All patients were able to void properly. Major complications were not observed. The procedure is technically demanding, resulting in long operative times at the first interventions.

  18. Early postoperative outcome of bipolar transurethral enucleation and resection of the prostate.

    Science.gov (United States)

    Cho, C L; Leung, Clarence L H; Chan, Wayne K W; Chu, Ringo W H; Law, I C

    2015-12-01

    To report the early postoperative outcome of bipolar transurethral enucleation and resection of the prostate. Our results were compared with those published from various centres. Regional hospital, Hong Kong. A total of 28 consecutive patients who had undergone bipolar transurethral enucleation and resection of the prostate by a single surgeon between January and June 2014. All patients were evaluated preoperatively by physical examination, digital rectal examination, transrectal ultrasonography, and laboratory studies, including measurement of haemoglobin, sodium, and prostate-specific antigen levels. Patients were assessed perioperatively and at 4 weeks and 3 months postoperatively. The mean resected specimen weight of prostatic adenoma in 28 patients was 48.2 g with a mean enucleation and resection time of 13.6 and 47.7 minutes, respectively. There was a mean decrease in serum prostate-specific antigen by 85.9% (from 6.4 ng/mL to 0.9 ng/mL) postoperatively. Prostate volume was decreased by 68.2% (from 71.9 cm(3) to 22.9 cm(3)) at 4 weeks postoperatively. The mean postoperative haemoglobin drop was 11.5 g/L. The rate of transient urinary incontinence at 3 months was 3.6%. Patients who underwent bipolar transurethral enucleation and resection of the prostate had a short catheterisation time and hospital stay, which is comparable to conventional transurethral resection of the prostate. Bipolar transurethral enucleation and resection of the prostate should become the endourological equivalent to open adenomectomy with fewer complications and short convalescence. The technique of bipolar transurethral enucleation and resection of the prostate can be acquired safely with a relatively short learning curve.

  19. Rapid Changes in Cortical and Subcortical Brain Regions after Early Bilateral Enucleation in the Mouse.

    Directory of Open Access Journals (Sweden)

    Olga O Kozanian

    Full Text Available Functional sensory and motor areas in the developing mammalian neocortex are formed through a complex interaction of cortically intrinsic mechanisms, such as gene expression, and cortically extrinsic mechanisms such as those mediated by thalamic input from the senses. Both intrinsic and extrinsic mechanisms are believed to be involved in cortical patterning and the establishment of areal boundaries in early development; however, the nature of the interaction between intrinsic and extrinsic processes is not well understood. In a previous study, we used a perinatal bilateral enucleation mouse model to test some aspects of this interaction by reweighting sensory input to the developing cortex. Visual deprivation at birth resulted in a shift of intraneocortical connections (INCs that aligned with ectopic ephrin A5 expression in the same location ten days later at postnatal day (P 10. A prevailing question remained: Does visual deprivation first induce a change in gene expression, followed by a shift in INCs, or vice versa? In the present study, we address this question by investigating the neuroanatomy and patterns of gene expression in post-natal day (P 1 and 4 mice following bilateral enucleation at birth. Our results demonstrate a rapid reduction in dorsal lateral geniculate nucleus (dLGN size and ephrin A5 gene expression 24-hours post-enucleation, with more profound effects apparent at P4. The reduced nuclear size and diminished gene expression mirrors subtle changes in ephrin A5 expression evident in P1 and P4 enucleated neocortex, 11 and 8 days prior to natural eye opening, respectively. Somatosensory and visual INCs were indistinguishable between P1 and P4 mice bilaterally enucleated at birth, indicating that perinatal bilateral enucleation initiates a rapid change in gene expression (within one day followed by an alteration of sensory INCs later on (second postnatal week. With these results, we gain a deeper understanding of how gene

  20. Holmium laser enucleation versus transurethral resection of the prostate.

    Science.gov (United States)

    Eltabey, Magdy A; Sherif, Hammouda; Hussein, Alaa A

    2010-12-01

    This was a prospective, randomized clinical trial to compare the safety, efficacy, and medium-term durability of holmium laser enucleation of the prostate (HoLEP) combined with mechanical morcellation versus standard transurethral resection of the prostate (TURP) for the surgical treatment of patients with bladder outlet obstruction due to benign prostatic hyperplasia (BPH). The patients had prostates that were greater than 30 g and less than 100 g and were followed for 1 year. From April 2008 to December 2009, 80 consecutive patients with lower urinary tract obstruction (LUTS) due to BPH were randomized to either surgical treatment with HoLEP (group 1, n = 40) or standard TURP (group 2, n = 40). Preoperative assessments included American Urological Association (AUA) symptom score, serum prostate-specific antigen (PSA), post-voiding residual (PVR) urine volume, transrectal ultrasound (TRUS), and urodynamic studies. Perioperative parameters included total operating time, resected tissue weight, hemoglobin loss, presence or absence of blood transfusion, time of catheter removal, and duration of hospital stay. Postoperative evaluations were conducted at 1, 6, and 12 months. Patients in the HoLEP group had shorter catheterization times and hospital stays than patients in the TURP group. There was no significant difference in operating times between the two groups. Mean hemoglobin loss was lower in the HoLEP group (1.8 ± 1.3 g/dL versus 2.9 ± 1.5 g/dL). There was a significantly greater improvement from baseline AUA symptom scores and PVR urine volumes in the HoLEP group versus the TURP group, at all postoperative assessments. Postoperatively, 25% of patients in group 1 (HoLEP) and 20% of patients in group 2 (TURP) had irritative voiding symptoms. Urethral stricture occurred in three cases (one case in the HoLEP group and two cases in the TURP group). HoLEP proved to be a safe and highly effective technique for surgical treatment of bladder outlet obstruction due to

  1. Effects of ocular enucleation on the oxidative metabolism of CNS structures and ovarium in female rats.

    Science.gov (United States)

    López, B D; Menéndez-Patterson, A; Marín, B

    1980-01-01

    In this paper we have studied oxygen uptake of the amygdala, hypothalamus, septal area, anterior cortex (latero-frontal), posterior cortex (latero-occipital) and ovarium in female rats in the oestrous and dioestrous phases with occular enculeation. Our results point to a statistically significant difference in the oestrous phase of the posterior cortex and ovarium levels, comparing control rats with the enucleated ones. In the dioestrous phase, the modifications that we have encountered are at the amygdala and ovarium. We point out the possible participation of the pineal gland in the control of the sexual cycle in in enucleated rats.

  2. Examining Stress in Graduate Assistants: Combining Qualitative and Quantitative Survey Methods

    Science.gov (United States)

    Mazzola, Joseph J.; Walker, Erin J.; Shockley, Kristen M.; Spector, Paul E.

    2011-01-01

    The aim of this study was to employ qualitative and quantitative survey methods in a concurrent mixed model design to assess stressors and strains in graduate assistants. The stressors most frequently reported qualitatively were work overload, interpersonal conflict, and organizational constraints; the most frequently reported psychological…

  3. Flower-like CuO synthesized by CTAB-assisted hydrothermal method

    Indian Academy of Sciences (India)

    Flower-like CuO nanostructures have been synthesized by cetyltrimethylammonium bromide (CTAB)-assisted hydrothermal method. Here, CuCl2.2H2O was used as copper raw material, and sodium hydroxide was used as precipitate. The resulting CuO powders were characterized by X-ray diffraction (XRD) and field ...

  4. Prioritizing humanitarian assistance in a complex emergency: a decision method for military forces

    NARCIS (Netherlands)

    Rietjens, S.J.H.; de Boer, S.J.; Voordijk, Johannes T.

    2004-01-01

    The article develops a decision support model for a military commander who has to determine what humanitarian assistance will be provided in cooperation with which civil organizations in peacekeeping situations. After an investigation of the current methods decision theory was used to develop an

  5. Efficiency of Computer Assisted Cooperative Learning Method on Students' Performance in Using Colors

    Science.gov (United States)

    Dikici, Ayhan

    2005-01-01

    In this study, the effects of computer assisted cooperative learning method on students' performance of using colours in art education were examined. 15 students at the ages between 12 and 15 who owned a personal computer and had access to the internet were selected to form the experimental group. 35 students at the same time age and education…

  6. A Method for Selection of Appropriate Assistive Technology for Computer Access

    Science.gov (United States)

    Jenko, Mojca

    2010-01-01

    Assistive technologies (ATs) for computer access enable people with disabilities to be included in the information society. Current methods for assessment and selection of the most appropriate AT for each individual are nonstandardized, lengthy, subjective, and require substantial clinical experience of a multidisciplinary team. This manuscript…

  7. Matrix assisted ionization in vacuum, a sensitive and widely applicable ionization method for mass spectrometry.

    Science.gov (United States)

    Trimpin, Sarah; Inutan, Ellen D

    2013-05-01

    An astonishingly simple new method to produce gas-phase ions of small molecules as well as proteins from the solid state under cold vacuum conditions is described. This matrix assisted ionization vacuum (MAIV) mass spectrometry (MS) method produces multiply charged ions similar to those that typify electrospray ionization (ESI) and uses sample preparation methods that are nearly identical to matrix-assisted laser desorption/ionization (MALDI). Unlike these established methods, MAIV does not require a laser or voltage for ionization, and unlike the recently introduced matrix assisted ionization inlet method, does not require added heat. MAIV-MS requires only introduction of a crystalline mixture of the analyte incorporated with a suitable small molecule matrix compound such as 3-nitrobenzonitrile directly to the vacuum of the mass spectrometer. Vacuum intermediate pressure MALDI sources and modified ESI sources successfully produce ions for analysis by MS with this method. As in ESI-MS, ion formation is continuous and, without a laser, little chemical background is observed. MAIV, operating from a surface offers the possibility of significantly improved sensitivity relative to atmospheric pressure ionization because ions are produced in the vacuum region of the mass spectrometer eliminating losses associated with ion transfer from atmospheric pressure to vacuum. Mechanistic aspects and potential applications for this new ionization method are discussed.

  8. The microwave-assisted ionic-liquid method: a promising methodology in nanomaterials.

    Science.gov (United States)

    Ma, Ming-Guo; Zhu, Jie-Fang; Zhu, Ying-Jie; Sun, Run-Cang

    2014-09-01

    In recent years, the microwave-assisted ionic-liquid method has been accepted as a promising methodology for the preparation of nanomaterials and cellulose-based nanocomposites. Applications of this method in the preparation of cellulose-based nanocomposites comply with the major principles of green chemistry, that is, they use an environmentally friendly method in environmentally preferable solvents to make use of renewable materials. This minireview focuses on the recent development of the synthesis of nanomaterials and cellulose-based nanocomposites by means of the microwave-assisted ionic-liquid method. We first discuss the preparation of nanomaterials including noble metals, metal oxides, complex metal oxides, metal sulfides, and other nanomaterials by means of this method. Then we provide an overview of the synthesis of cellulose-based nanocomposites by using this method. The emphasis is on the synthesis, microstructure, and properties of nanostructured materials obtained through this methodology. Our recent research on nanomaterials and cellulose-based nanocomposites by this rapid method is summarized. In addition, the formation mechanisms involved in the microwave-assisted ionic-liquid synthesis of nanostructured materials are discussed briefly. Finally, the future perspectives of this methodology in the synthesis of nanostructured materials are proposed. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Holmium laser enucleation of the prostate: surgical, functional, and quality-of-life outcomes upon extended follow-up

    Science.gov (United States)

    Alkan, Ilter; Ozveri, Hakan; Akin, Yigit; Ipekci, Tumay; Alican, Yusuf

    2016-01-01

    ABSTRACT Objectives: To evaluate the long-term surgical, functional, and quality-of-life (QoL) outcomes after Holmium laser enucleation of the prostate (HoLEP) in patients with symptomatic benign prostatic hyperplasia (BPH). Materials and Methods: We retrospectively reviewed recorded data on patients who underwent HoLEP between June 2002 and February 2005. Ninety-six patients were enrolled. Demographic, perioperative, and postoperative data were recorded. On follow-up, International Prostate Symptom Scores (IPSSs), prostate-specific antigen (PSA) levels, QoL scores, peak uroflowmetric data (Qmax values), and post-voiding residual urine volumes (PVR volumes), were recorded. Complications were scored using the Clavien system. Statistical significance was set at ptransurethral resection of the prostate. PMID:27256184

  10. Comparing Antibacterial and Antioxidant Activity of Annatto Dye Extracted by Conventional and Ultrasound-Assisted Methods

    OpenAIRE

    Mahmoud Yolmeh; Mohammad Bagher Habibi-Najafi; Shahrzad Shakouri; Fereshteh Hosseini

    2015-01-01

    Background: Annatto dye is used extensively in food industry that has antibacterial and antioxidant properties. Objectives: Aim of this paper was comparison of the antibacterial and antioxidant properties of annatto dye was extracted by conventional and ultrasound-assisted extraction (UAE) methods. Materials and Methods: In this experimental study minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of annatto dye against the bacteria were determined by ag...

  11. Dilation-assisted stone extraction: an alternative method for removal of common bile duct stones.

    Science.gov (United States)

    Li, Guodong; Pang, Qiuping; Zhang, Xiujuan; Dong, Haiyan; Guo, Rong; Zhai, Hailan; Dong, Yanchun; Jia, Xinyong

    2014-04-01

    Dilation-assisted stone extraction, also termed small endoscopic sphincterotomy (EST) plus endoscopic papillary balloon dilatation, is more efficient than EST alone for removal of large common bile duct (CBD) stones. However, whether this technique can be used for all stones is unclear. This study was designed to evaluate the efficacy and complications of dilation-assisted stone extraction for CBD stones. A total of 462 patients with CBD stones were randomized to undergo either dilation-assisted stone extraction (group A) or EST (group B). The efficacy and complications of the two techniques were compared. Groups A and B showed similar outcomes in terms of stone removal. The short-term and 1-year complication rates were also similar between the two groups. However, the first-session stone removal rate in group A was significantly higher than that in group B. Mechanical lithotripsy was required significantly more often in group B than in group A. The total procedure time and total fluoroscopy time in group A were significantly shorter than those in group B. Dilation-assisted stone extraction and EST are safe and effective techniques for the treatment of CBD stones. Dilation-assisted stone extraction has high efficiency. This technique is an alternative method for removal of CBD stones.

  12. Prevention of Evisceration or Enucleation in Endogenous Bacterial Panophthalmitis with No Light Perception and Scleral Abscess.

    Science.gov (United States)

    Chen, Kuan-Jen; Chen, Yen-Po; Chao, An-Ning; Wang, Nan-Kai; Wu, Wei-Chi; Lai, Chi-Chun; Chen, Tun-Lu

    2017-01-01

    Panophthalmitis is the most extensive ocular involvement in endophthalmitis with inflammation in periocular tissues. Severe inflammation of the anterior and posterior segments is frequently accompanied by corneal opacity, scleral abscess, and perforation or rupture. Enucleation or evisceration was the only remaining viable treatment option when all options to salvage the eye had been exhausted. The purpose of this retrospective study is to examine the outcomes of patients with endogenous bacterial panophthalmitis, no light perception and scleral abscess who were treated with multiple intravitreal and periocular injections of antibiotics and dexamethasone. Evaluation included spreading of infection to contiguous or remote sites, following evisceration or enucleation, and sympathetic ophthalmia. Eighteen patients were diagnosed with EBP, with liver abscesses in eight patients, retroperitoneal infection in four, pneumonia in two, infective endocarditis in one, cellulitis in one, drug abuse in one, and mycotic pseudoaneurysm in one. Culture results were positive for Klebsiella pneumoniae in 12 patients, Streptococcus spp. in three, Pseudomonas aeruginosa in one, Escherichia coli in one, and Staphylococcus aureus in one. The average number of periocular injections was 2.2, and the average number of intravitreal injections was 5.8. No eye required evisceration or enucleation and developed the spreading of infection to contiguous or remote sites during the follow-up. No sympathetic ophthalmia was observed in the fellow eye of all patients. Prevention of evisceration or enucleation in patients with EBP, NLP and scleral abscess can be achieved by multiple intravitreal and periocular injections of antibiotics and dexamethasone.

  13. Dermal-fat graft for anophthalmic socket in children enucleated for retinoblastoma.

    Science.gov (United States)

    Bosch-Canto, V; Cruz, C; Ordaz-Favila, J C

    2018-01-01

    Retinoblastoma is the most frequent intraocular tumour in childhood. The definitive treatment is enucleation. The management of the anophthalmic socket consists in the use of a plastic implant. The problem is that they are expensive and they usually extrude. The use of dermal-fat grafts minimises the hemi-facial hypoplasia. They usually grow with the face, and help to expand the orbital bones, thus avoiding the psychological and physical consequences. To determine if there is hemi-facial hypoplasia, using MRI images after the use of a dermal-fat implant in patients enucleated for RB. The study included patients enucleated for RB in which a dermal-fat implant was used and MRI images were taken in the period between June 2010 and December 2012. Facial growth and cosmesis was measured. The study included 12 patients, aged between 6 to 41 months. After 24 months of follow up, none of them developed hemifacial hypoplasia. All had a good cosmesis with the prosthesis. There were no complications after the surgery. The use of dermal-fat implant is a good option for the anophthalmic socket in patients with RB after enucleation. Copyright © 2017 Sociedad Española de Oftalmología. Publicado por Elsevier España, S.L.U. All rights reserved.

  14. Comparison of Microwave-Assisted and Hydrodistillation Methods for Extraction of Essential Oil from Achillea millefolium

    Directory of Open Access Journals (Sweden)

    S. Mollasalehi

    2013-08-01

    Full Text Available Microwave-assisted hydrodistillation (MAHD method has been compared with hydrodistillation (HD conventional technique for extraction of essential oil from Achillea millefolium. Microwave-assisted hydrodistillation were examined at three levels of microwave powers (300, 500, and 700 W. Obtained results show that MAHD offers important advantages over HD in terms of energy savings and extraction time (20 min against 2.5 h. Also, the essential oils were analyzed by GC-MS. The amount of oxygenated compounds and monoterpene, such as 1,8 -Cineole, Lavandulyl acetate,Caryophylla-dien, Aromadendrene were increased in the microwave method. All these results suggest that MAHD represents an excellent alternative method for extraction of essential oils from plant materials.

  15. Orbital Implants in Enucleation Surgery: A Report by the American Academy of Ophthalmology.

    Science.gov (United States)

    Wladis, Edward J; Aakalu, Vinay K; Sobel, Rachel K; Yen, Michael T; Bilyk, Jurij R; Mawn, Louise A

    2018-02-01

    To compare the motility and complication rates of porous and nonporous implants after enucleation surgery. Literature searches of the PubMed and Cochrane Library databases were last performed in February 2017 to identify studies published between 2003 and 2017 on outcomes after enucleation surgeries in which a variety implants were used. The searches were limited to the English language with abstracts and yielded 43 articles, which the Ophthalmic Technology Assessment Committee Oculoplastics and Orbit Panel reviewed for relevancy. Twenty-five articles were considered to have met the search strategy, and the panel methodologist assigned ratings to them according to the level of evidence. Only 2 of the 25 articles identified met the criteria for level I evidence. Eighteen of the studies did not assess motility after enucleation surgery, and the 7 that did evaluate this metric involved porous implants. The studies that analyzed this outcome reported favorable results, but the results were not uniformly based on objective analysis. Both porous and nonporous implants were well tolerated, and complication rates were generally low for both types. In keeping with increasing surgeon preference for porous implants, most studies identified in this literature search involved the use of this type of implant. These implants resulted in excellent motility after enucleation surgery, although many studies did not assess this outcome. Regardless of implant type, major complications were rare, and infection was exceptionally uncommon after enucleation. Given the paucity of data on motility and the absence of direct, objective comparisons of porous and nonporous implants, definitive conclusions about the impact of implant material on motility cannot be made. Since few studies evaluated nonporous implants, direct comparisons cannot be made definitively between implant types, and future investigations are needed to enable a critical assessment. Copyright © 2017 American Academy of

  16. Enucleation versus Anatomic Resection for Giant Hepatic Hemangioma: A Meta-Analysis.

    Science.gov (United States)

    Liu, Yuhui; Wei, Xuyong; Wang, Kun; Shan, Qiaonan; Dai, Haojiang; Xie, Haiyang; Zhou, Lin; Xu, Xiao; Zheng, Shusen

    2017-03-01

    Hepatic hemangiomas are the most common benign liver tumors, and the management of giant hepatic hemangioma (GHH) is still in controversial. The aim of this meta-analysis was to compare the postoperative outcomes of enucleation versus anatomic resection for GHH. PubMed, Embase, Web of Science, and the Cochrane Library were searched from January 1988 to December 2015 to identify studies comparing the outcomes of enucleation versus anatomic resection for GHH. Finally, we performed this meta-analysis using the Review Manager 5.3 software, and the results were presented as risk ratio (RR) or mean difference (MD) with corresponding 95% confidence interval (CI). The major limitation is that all data were derived from nonrandomized studies, and we cannot exclude potential selection bias. Nine studies involving 1,185 patients were included. The results showed that there was a lower incidence of complications (RR = 0.66, 95% CI 0.52 to 0.84, I(2) = 0%, p = 0.0007); no incidents of death occurred among the 9 included trials. Blood loss (MD = -419.07 mL, 95% CI -575.04 to -263.09, I(2) = 83%, p < 0.00001), duration of surgery (MD = -23.5 min, 95% CI -45.28 to -1.74, I(2) = 0%, p = 0.03), and length of hospital stay (MD = -1.59 days, 95% CI -3.06 to -0.13, I(2) = 0%, p = 0.03) were much lower in the enucleation group. GHH can be removed safely by either enucleation or anatomic resection. Enucleation can preserve more hepatic parenchyma and reduce postoperative complications, which is why it should be the preferred surgical procedure for suitable lesions.

  17. Extrascleral extension of choroidal melanoma: post-enucleation high-dose-rate interstitial brachytherapy of the orbit.

    Science.gov (United States)

    Finger, Paul T; Tena, Lawrence B; Semenova, Ekaterina; Aridgides, Paul; Choi, Walter H

    2014-01-01

    To investigate if orbital extension of uveal melanoma can be treated with high-dose-rate (HDR) brachytherapy. This study is a retrospective analysis of the results of a clinical case series was performed on 10 patients. Each underwent primary enucleation for uveal melanoma, was discovered to have orbital extension, and consented for HDR brachytherapy. By American Joint Committee on Cancer (AJCC) initial tumor grading, there was one each (T1c, T2c, T2d, and T3d, three T4c, and two T4d-staged uveal melanomas. One was AJCC-staged R2 due to orbital recurrence presenting 16 months after enucleation. (192)Ir HDR brachytherapy involved transcutaneous circumferential orbital incisions allowing for evenly spaced brachytherapy catheters into the orbit. A target dose of 32.85 Gy (range, 32.85-34 Gy) was delivered in 9-10 twice-daily fractions (range, 3.4-3.65 Gy per fraction) over 5 consecutive days. Data analysis included but was not limited to radiation therapy methods, local tumor control, side effects, and metastatic rate. In the 9 patients who tolerated treatment, there has been no orbital recurrence at a median follow-up of 18 months (range, 1-62 months). Four patients died of metastatic disease (one presented with a treated solitary liver metastasis before brachytherapy). There was no significant eyelash or eyebrow loss. There was no radiation-induced eyelid erythema, orbital infection, or contracted sockets. All orbits accepted and maintained ocular prostheses. Brachytherapy was used as an alternative to external beam radiation treatment for postenucleation orbital melanoma. This series reports complete local control, few side effects, and excellent cosmetic results. Copyright © 2014 American Brachytherapy Society. Published by Elsevier Inc. All rights reserved.

  18. Age and prostate volume are risk factors for transient urinary incontinence after transurethral enucleation with bipolar for benign prostatic hyperplasia.

    Science.gov (United States)

    Hirasawa, Yosuke; Kato, Yuji; Fujita, Kiichiro

    2017-10-03

    To investigate the predictive factors for transient urinary incontinence after transurethral enucleation with bipolar. We retrospectively analyzed the data of 584 patients who underwent transurethral enucleation with bipolar between December 2011 and September 2016 operated by a single surgeon. Urinary incontinence after transurethral enucleation with bipolar was defined as involuntary leakage of urine that required the use of pads. It was evaluated at 1 week, and 1, 3, 6, 12 and 24 months after transurethral enucleation with bipolar. We defined transient urinary incontinence as urinary incontinence persisting up to 1 month after transurethral enucleation with bipolar. Based on independent risk factors identified by a multivariate stepwise logistic regression analysis, a nomogram to predict transient urinary incontinence was developed. Of the 584 patients, 17.3%, 13.5%, 3.1%, 0.41%, and 0% patients had urinary incontinence at 1 week, 1, 3, 6 and 12 months after transurethral enucleation with bipolar, respectively. The mean (±standard error) age was 69.6 ± 0.26 years, estimated prostate volume was 54.7 ± 0.91 cm3 , operative time was 58.0 ± 1.1 min and the prostate specimen weight was 30.6 ± 0.69 g. On univariate analysis, age, prostate volume estimated by transrectal ultrasonography, prostate-specific antigen, prostate specimen weight, operative time, prostate specimen weight/prostate volume and prostate specimen weight/operative time were significant predictive factors for transient urinary incontinence after transurethral enucleation with bipolar. On multivariate analysis, age (hazard ratio 1.07, P-value = 0.0034) and prostate volume (hazard ratio 1.03, P-value transurethral enucleation with bipolar. Age and prostate volume estimated by transrectal ultrasonography seem to represent significant independent risk factors for transient urinary incontinence after transurethral enucleation with bipolar. This should be well discussed with the patient before surgery

  19. PERFORMANCE OF TRAPEZOIDAL METHOD AND GAUSS-LEGENDRE METHOD IN SETTLEMENT OF CERTAIN INTEGRAL ASSISTED MATLAB

    Directory of Open Access Journals (Sweden)

    Adi Prasetia

    2016-10-01

    Full Text Available This study aimed to compare the performance of a particular integral calculation using the Trapezoid and Gauss-Legendre method in five integral cases to determine the accuracy of the integral results value of both methods which are based on the relative error percentage. This study is a literature research which formulate mathematical problem that can be solved with the usual arithmetic operations or calculations which are addition, subtraction, multiplication and division to obtain numeral with the best accuracy. Trapezoidal method formula used is while the Gauss-Legendre formula used is , This study shows that the Gauss-Legendre method has better performance by giving the percentage of error which is relatively better than Trapezoid method used in the five cases. Keywords: Numerical Methods, Trapezoid method, the method of Gauss-Legendre

  20. Membrane contactor assisted water extraction system for separating hydrogen peroxide from a working solution, and method thereof

    Science.gov (United States)

    Snyder, Seth W [Lincolnwood, IL; Lin, Yupo J [Naperville, IL; Hestekin', Jamie A [Fayetteville, AR; Henry, Michael P [Batavia, IL; Pujado, Peter [Kildeer, IL; Oroskar, Anil [Oak Brook, IL; Kulprathipanja, Santi [Inverness, IL; Randhava, Sarabjit [Evanston, IL

    2010-09-21

    The present invention relates to a membrane contactor assisted extraction system and method for extracting a single phase species from multi-phase working solutions. More specifically one preferred embodiment of the invention relates to a method and system for membrane contactor assisted water (MCAWE) extraction of hydrogen peroxide (H.sub.2O.sub.2) from a working solution.

  1. Factors affecting de novo urinary retention after Holmium laser enucleation of the prostate.

    Directory of Open Access Journals (Sweden)

    Sung Han Kim

    Full Text Available OBJECTIVE: Patients can experience urinary retention (UR after Holmium laser enucleation of the prostate (HoLEP that requires bladder distension during the procedure. The aim of this retrospective study is to identify factors affecting the UR after HoLEP. MATERIALS AND METHODS: 336 patients, which underwent HoLEP for a symptomatic benign prostatic hyperplasia between July 2008 and March 2012, were included in this study. Urethral catheters were routinely removed one or two days after surgery. UR was defined as the need for an indwelling catheter placement following a failure to void after catheter removal. Demographic and clinical parameters were compared between the UR (n = 37 and the non-urinary retention (non-UR; n = 299 groups. RESULTS: The mean age of patients was 68.3 (±6.5 years and the mean operative time was 75.3 (±37.4 min. Thirty seven patients (11.0% experienced a postoperative UR. UR patients voided catheter free an average of 1.9 (±1.7 days after UR. With regard to the causes of UR, 24 (7.1% and 13 (3.9% patients experienced a blood clot-related UR and a non-clot related UR respectively. Using multivariate analysis (p0.05. CONCLUSIONS: De novo UR after HoLEP was found to be self-limited and it was not related to learning curve, patient age, diabetes, or operative time. Efficient morcellation and careful control of bleeding, which reduces clot formation, decrease the risk of UR after HoLEP.

  2. Matrix Assisted Ionization Vacuum (MAIV), a New Ionization Method for Biological Materials Analysis Using Mass Spectrometry*

    Science.gov (United States)

    Inutan, Ellen D.; Trimpin, Sarah

    2013-01-01

    The introduction of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) for the mass spectrometric analysis of peptides and proteins had a dramatic impact on biological science. We now report that a wide variety of compounds, including peptides, proteins, and protein complexes, are transported directly from a solid-state small molecule matrix to gas-phase ions when placed into the vacuum of a mass spectrometer without the use of high voltage, a laser, or added heat. This ionization process produces ions having charge states similar to ESI, making the method applicable for high performance mass spectrometers designed for atmospheric pressure ionization. We demonstrate highly sensitive ionization using intermediate pressure MALDI and modified ESI sources. This matrix and vacuum assisted soft ionization method is suitable for the direct surface analysis of biological materials, including tissue, via mass spectrometry. PMID:23242551

  3. Water-assisted and surfactant-free synthesis of cobalt ferrite nanospheres via solvothermal method

    Energy Technology Data Exchange (ETDEWEB)

    Bi, Yiqing [CAS Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190 (China); University of the Chinese Academy of Sciences, Beijing 100049 (China); Ren, Yanan [CAS Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190 (China); Bi, Feng [CAS Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190 (China); University of the Chinese Academy of Sciences, Beijing 100049 (China); He, Tao, E-mail: het@nanoctr.cn [CAS Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, Beijing 100190 (China)

    2015-10-15

    With ethylene glycol as the solvent, monodispersed cobalt ferrite nanospheres were prepared via a solvothermal method assisted by water. The samples were mainly characterized by X-ray diffraction, scanning electron microscope, and transmission electron microscope. The size of as-prepared products ranges from 10 nm to 200 nm. Size distribution and chemical composition were controlled by the amount of water and pH value in the reaction system. More important, suitable amount of water can avoid the use of surfactant. - Highlights: • Cobalt ferrite nanospheres were synthesized via solvothermal method assisted by water. • An introduction of suitable amount of water can avoid the use of surfactant. • The pH value of the precursor can be used to adjust the product composition.

  4. Transurethral anatomical enucleation of the prostate with Tm:YAG support (ThuLEP): review of the literature on a novel surgical approach in the management of benign prostatic enlargement.

    Science.gov (United States)

    Kyriazis, Iason; Świniarski, Piotr P; Jutzi, Stephan; Wolters, Mathias; Netsch, Christopher; Burchardt, Martin; Liatsikos, Evangelos; Xia, Shujie; Bach, Thorsten; Gross, Andreas J; Herrmann, Thomas R W

    2015-04-01

    Retrograde transurethral anatomical enucleation of the prostate is gaining momentum as a new concept in transurethral surgery of benign prostatic hyperplasia. Its adaptation is boosted by the familiarity of urologists with the finger-assisted anatomical enucleation of the adenoma during open prostatectomy and the combination of this well-established concept with the minimal invasive characteristics of transurethral surgery. The thulium laser appears as an ideal energy source for such operation. In this work, current evidence on thulium laser-assisted anatomical enucleation of the prostate (ThuLEP) is being reviewed. A comprehensive literature review was performed on Medline, PubMed, and Cochrane databases retrieving all literature on thulium laser-assisted prostatectomy between 2006 and 2015. Experimental studies, review articles and editorial comments as well as studies on thulium laser-assisted approaches other than ThuLEP (i.e., ThuVEP, ThuVAP or ThuVARP) were excluded from the analysis. In total, six original articles on either surgical technique or clinical outcomes were retrieved. With regard to functional results, ThuLEP presented no significant differences toward the standard treatment (TURP/HoLEP) arm in two randomized controlled trials and favorable outcomes in available prospective cohorts. Observed morbidity was minimum and comparable with the rest of transurethral literature. ThuLEP literature is still very limited. Based on the available data, the approach is safe and effective, demonstrating favorable outcomes, comparable with the current standard treatment options. Further documentation of ThuLEP outcomes is necessary to define the optimum indications of this novel technique.

  5. Optical characterization of europium-doped indium hydroxide nanocubes obtained by Microwave-Assisted Hydrothermal method

    OpenAIRE

    Motta, Fabiana Villela da; Marques,Ana Paula de Azevedo; Araújo,Vinícius Dantas de; Tavares, Mara Tatiane De Souza; Delmonte,Mauricio Roberto Bomio; Paskocimas, Carlos Alberto; Li, Máximo Siu; Nascimento, Rubens Maribondo do; Longo, Elson [UNESP

    2014-01-01

    Crystalline europium-doped indium hydroxide (In(OH)3:Eu) nanostructures were prepared by rapid and efficient Microwave-Assisted Hydrothermal (MAH) method. Nanostructures were obtained at low temperature. FE-SEM images confirm that these samples are composed of 3D nanostructures. XRD, optical diffuse reflectance and photoluminescence (PL) measurements were used to characterize the products. Emission spectra of europium-doped indium hydroxide (IH:xEu) samples under excitation (350.7 nm) present...

  6. A Study of Polishing Feature of Ultrasonic-Assisted Vibration Method in Bamboo Charcoal

    Directory of Open Access Journals (Sweden)

    Hsin-Min Lee

    2017-01-01

    Full Text Available Focusing on the feature of porosity in bamboo charcoal, this study applies the ultrasonic-assisted vibration method to perform surface polishing of the silicon wafer workpiece. The self-developed bamboo charcoal polishing spindle and ultrasonic- assisted vibration mechanism are attached to a single lapping machine. In the machining process, ultrasonic vibration enables the diamond slurry to smoothly pass through the microscopic holes of bamboo charcoal; the end of the bamboo charcoalis able to continue machining on the surface of the workpiece through the grasping force which exists in the microscopic holes. Under the polishing and machining parameters of ultrasonic-assisted vibration, with a diamond slurry concentration of 0.3%, the experimental results show a polishing time of 20 min, a loading of 25 N on the workpiece surface, a spindle speed of 1200 rpm, a vibration frequency of 30 kHz and the original surface roughness value of Ra 0.252 μm equals that of a mirror-like surface at Ra 0.017 μm. These research results prove that by using bamboo charcoal and ultrasonic-assisted vibration for polishing, a very good improvement can be achieved on the workpiece surface.

  7. Chemometrics-assisted spectrophotometry method for the determination of chemical oxygen demand in pulping effluent.

    Science.gov (United States)

    Chen, Honglei; Chen, Yuancai; Zhan, Huaiyu; Fu, Shiyu

    2011-04-01

    A new method has been developed for the determination of chemical oxygen demand (COD) in pulping effluent using chemometrics-assisted spectrophotometry. Two calibration models were established by inducing UV-visible spectroscopy (model 1) and derivative spectroscopy (model 2), combined with the chemometrics software Smica-P. Correlation coefficients of the two models are 0.9954 (model 1) and 0.9963 (model 2) when COD of samples is in the range of 0 to 405 mg/L. Sensitivities of the two models are 0.0061 (model 1) and 0.0056 (model 2) and method detection limits are 2.02-2.45 mg/L (model 1) and 2.13-2.51 mg/L (model 2). Validation experiment showed that the average standard deviation of model 2 was 1.11 and that of model 1 was 1.54. Similarly, average relative error of model 2 (4.25%) was lower than model 1 (5.00%), which indicated that the predictability of model 2 was better than that of model 1. Chemometrics-assisted spectrophotometry method did not need chemical reagents and digestion which were required in the conventional methods, and the testing time of the new method was significantly shorter than the conventional ones. The proposed method can be used to measure COD in pulping effluent as an environmentally friendly approach with satisfactory results.

  8. Metal oxide targets produced by the polymer-assisted deposition method

    Energy Technology Data Exchange (ETDEWEB)

    Garcia, Mitch A., E-mail: mitch@berkeley.ed [Department of Chemistry, Room 446 Latimer Hall, University of California Berkeley, Berkeley, CA 94720-1460 (United States); Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Ali, Mazhar N.; Chang, Noel N.; Parsons-Moss, T. [Department of Chemistry, Room 446 Latimer Hall, University of California Berkeley, Berkeley, CA 94720-1460 (United States); Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Ashby, Paul D. [Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Gates, Jacklyn M. [Department of Chemistry, Room 446 Latimer Hall, University of California Berkeley, Berkeley, CA 94720-1460 (United States); Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Stavsetra, Liv [Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Gregorich, Kenneth E.; Nitsche, Heino [Department of Chemistry, Room 446 Latimer Hall, University of California Berkeley, Berkeley, CA 94720-1460 (United States); Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)

    2010-02-11

    The polymer-assisted deposition (PAD) method was used to create crack-free homogenous metal oxide films for use as targets in nuclear science applications. Metal oxide films of europium, thulium, and hafnium were prepared as models for actinide oxides. Films produced by a single application of PAD were homogenous and uniform and ranged in thickness from 30 to 320 nm. Reapplication of the PAD method (six times) with a 10% by weight hafnium(IV) solution resulted in an equally homogeneous and uniform film with a total thickness of 600 nm.

  9. A Review of Smart House Analysis Methods for Assisting Older People Living Alone

    Directory of Open Access Journals (Sweden)

    Veralia Gabriela Sanchez

    2017-07-01

    Full Text Available Smart Houses are a prominent field of research referring to environments adapted to assist people in their everyday life. Older people and people with disabilities would benefit the most from the use of Smart Houses because they provide the opportunity for them to stay in their home for as long as possible. In this review, the developments achieved in the field of Smart Houses for the last 16 years are described. The concept of Smart Houses, the most used analysis methods, and current challenges in Smart Houses are presented. A brief introduction of the analysis methods is given, and their implementation is also reported.

  10. A study of thermal properties of sodium titanate nanotubes synthesized by microwave-assisted hydrothermal method

    Energy Technology Data Exchange (ETDEWEB)

    Preda, Silviu, E-mail: predas01@yahoo.co.uk [Institute of Physical Chemistry “Ilie Murgulescu”, 202 Splaiul Independentei, 060021 Bucharest (Romania); Rutar, Melita [Jožef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Jožef Stefan International Postgraduate School, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Umek, Polona [Jožef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana (Slovenia); Zaharescu, Maria [Institute of Physical Chemistry “Ilie Murgulescu”, 202 Splaiul Independentei, 060021 Bucharest (Romania)

    2015-11-15

    Highlights: • The microwave-assisted hydrothermal route was used for titanate nanotubes synthesis. • Conversion to single-phase nanotube morphology completes after 8 h reaction time. • The nanotube morphology is stable up to 600 °C, as determined by in-situ XRD and SEM. • Sodium ions migrate to the surface due to thermal motion and structure condensation. - Abstract: Sodium titanate nanotubes (NaTiNTs) were synthesized by microwave-assisted hydrothermal treatment of commercial TiO{sub 2}, at constant temperature (135 °C) and different irradiation times (15 min, 1, 4, 8 and 16 h). The products were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, differential scanning calorimetry and specific surface area measurements. The irradiation time turned out to be the key parameter for morphological control of the material. Nanotubes were observed already after 15 min of microwave irradiation. The analyses of the products irradiated for 8 and 16 h confirm the complete transformation of the starting TiO{sub 2} powder to NaTiNTs. The nanotubes are open ended with multi-wall structures, with the average outer diameter of 8 nm and specific surface area up to 210 m{sup 2}/g. The morphology, surface area and crystal structure of the sodium titanate nanotubes synthesized by microwave-assisted hydrothermal method were similar to those obtained by conventional hydrothermal method.

  11. Periodontal regenerative management of residual tunnel osseous defect results from the enucleation of lateral periodontal cyst in anterior maxilla: A rare case report

    Directory of Open Access Journals (Sweden)

    Sanjeev Kumar Salaria

    2016-01-01

    Full Text Available The lateral periodontal cyst (LPC is a nonkeratinized, noninflammatory developmental cyst occurring adjacent or lateral to tooth root. It is a relatively uncommon lesion found in the maxillary incisors and found mostly in adults during 5th to 7th decades. In this case, 45-year-old male patient reported with occasional mild discomfort between left maxillary central and lateral incisor region since 1 year. Interproximally, a well-defined round radiolucent area with corticated borders was determined radiographically between vital tooth #21 and #22. Preliminary diagnosis of LPC was established based on clinical and radiographical findings. Following enucleation of the lesion, an anticipated residual tunnel osseous defect was observed, which was managed successfully utilizing bone graft and guided tissue regeneration-assisted technique. Cystic tissue removed was examined histologically; hematoxylin- and eosin-stained sections showed features suggestive of LPC. Complete healing of tunnel defect was achieved at 1 year follow-up.

  12. A New Celestial Navigation Method for Spacecraft on a Gravity Assist Trajectory

    Directory of Open Access Journals (Sweden)

    Ning Xiaolin

    2013-01-01

    Full Text Available A practical and reliable capability for autonomous navigation needs to reduce operation cost, to improve operational efficiency, and to increase mission safety. Celestial navigation is a very attractive autonomous navigation solution for deep space spacecraft. There are mainly two kinds of celestial navigation methods: the direct calculation method and the filter method. The accuracy of the direct calculation method is low and very sensitive to the measurement noise. The filter method can provide a better navigation performance if a high accuracy dynamical model is available. However, the main practical problem existing in the autonomous celestial navigation of spacecraft on a gravity assist trajectory is that the accuracy of trajectory model is not enough to be used in the real navigation sometimes, which may introduce large estimation error and even cause filter divergence. To solve this problem, a new celestial navigation method is proposed in this paper, which effectively combines the direct calculation method and the filter method using an interacting multiple model unscented Kalman filter (IMMUKF. The ground experimental results demonstrate that this method can provide better navigation performance and higher reliability than the traditional direct calculation method and filter method.

  13. Risk of metastasis and orbital recurrence in advanced retinoblastoma eyes treated with systemic chemoreduction versus primary enucleation.

    Science.gov (United States)

    Berry, Jesse L; Kogachi, Kaitlin; Aziz, Hassan A; McGovern, Kathleen; Zolfaghari, Emily; Murphree, A Linn; Jubran, Rima; Kim, Jonathan W

    2017-04-01

    The purpose of this study was to evaluate the risk of metastatic disease and orbital recurrence in advanced retinoblastoma treated with systemic chemoreduction versus primary enucleation. A retrospective review of patients with Group D/E retinoblastoma was conducted with data collection from 1995 to 2015. Overall, 345 eyes (294 patients) were included (165 Group D and 180 Group E). Primary outcome measures were orbital recurrence and metastatic disease. Of the 345 eyes, 139 were treated with systemic chemoreduction (102 Group D, 37 Group E) and 206 with primary enucleation (63 Group D, 143 Group E). In the chemoreduction group, one patient developed metastasis (0.7%) and one an orbital recurrence (0.7%). In the primary enucleation group, two patients developed metastases (0.9%) and one an orbital recurrence (0.5%). After systemic chemoreduction, 58 of the 139 eyes (30 Group D, 28 Group E) were secondarily enucleated for treatment failure (41.7%). The median time to secondary enucleation from diagnosis was 8.1 months. None of the eyes in the systemic chemoreduction group had high-risk pathologic features. In the primary enucleation group, 56 eyes had high-risk pathology. Over a 20-year period, 345 eyes were treated for advanced retinoblastoma at Children's Hospital Los Angeles. Incidence of orbital recurrence and metastatic disease was <1% and did not vary by treatment modality or group classification. None of the eyes enucleated for treatment failure had high-risk pathology, and none of these patients developed metastatic disease. Globe salvage therapy with systemic chemoreduction and subsequent enucleation for poor response does not increase the risk of metastatic disease or orbital recurrence. © 2016 Wiley Periodicals, Inc.

  14. Case of vascular air embolism during holmium laser enucleation of the prostate.

    Science.gov (United States)

    Kato, Takuma; Sugimoto, Mikio; Matsuoka, Yuki; Sakura, Yuma; Hayashida, Yushi; Hirama, Hiromi; Tsunemori, Hiroyuki; Ueda, Nobufumi; Uemura, Naoya; Miyawaki, Yuki; Shirakami, Gotaro; Kakehi, Yoshiyuki

    2015-02-01

    Vascular air embolism is a rare complication during transurethral surgery. A case of air embolism during holmium laser enucleation of the prostate in a 76-year-old man is presented. During the step of morcellation, the patient's blood pressure suddenly oscillated up and down, and end-tidal CO2 and arterial saturation decreased. Transesophageal and transthoracic echocardiography showed air collection in the right atrium. It was also discovered that incorrect assembly of the tube from the morcellator caused rapid entrainment of air into the vein. Computed tomography and abdominal X-ray showed niveau formation in the femoral vein and air collection in the pelvic retroperitoneal space. The patient recovered with careful observation and was discharged 7 days after the operation with no sequelae. This report is presented to remind urologists of this unusual complication that can occur during holmium laser enucleation of the prostate procedures. © 2014 The Japanese Urological Association.

  15. Enucleation after Embolization of Liver Failure-Causing Giant Liver Hemangioma

    Science.gov (United States)

    Sharma, Amit; Kaspar, Matthew; Siddiqui, Mohammad; Kim, Joohyun

    2015-01-01

    Patient: Female, 73 Final Diagnosis: Giant liver hemangioma Symptoms: Abdominal discomfort • abdominal enlargement • Icterus Medication: — Clinical Procedure: Enucleation after embolization of liver failure-causing giant liver Specialty: Surgery Objective: Unusual clinical course Background: Hepatic hemangioma is a congenital tumor of the mesenchymal tissues of the liver. While typically benign, these tumors can occasionally grow to sufficient size to cause a number of symptoms, including pain, severe hepatic dysfunction, or, rarely, consumptive coagulopathy. In such instances, surgical treatment may be warranted. Case Report: We present a case of a symptomatic giant hepatic hemangioma in an elderly patient who presented with impending liver failure. She was successfully treated with a combination of surgical enucleation and liver resection after preoperative arterial embolization. We also provide a brief discussion of current treatment options for giant hepatic hemangiomas. Conclusions: Early referral to experienced surgical centers before the onset of dire complications such as severe hepatic dysfunction and liver failure is recommended. PMID:26301888

  16. Is there a way to predict stress urinary incontinence after holmium laser enucleation of the prostate?

    Science.gov (United States)

    Elmansy, Hazem M; Kotb, Ahmed; Elhilali, Mostafa M

    2011-11-01

    In this study we defined high risk patients at high risk of stress urinary incontinence after holmium laser enucleation of the prostate. We performed a retrospective analysis during a 10-year period of 949 consecutive patients treated with holmium laser enucleation of the prostate by a single surgeon. Patients were divided into group 1--those without postoperative stress urinary incontinence (902) and group 2--those with stress urinary incontinence (47). All preoperative, intraoperative and postoperative clinical variables were compared between the 2 groups. Patient age, preoperative and postoperative prostate specific antigen, preoperative medications, preoperative acute retention and duration of postoperative catheter time were not associated with postoperative stress urinary incontinence. The presence of diabetes mellitus was significantly associated with a higher incidence of stress urinary incontinence (p Kegel exercises in the immediate postoperative period. Copyright © 2011 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  17. Ball mill assisted rapid mechanochemical extraction method for natural products from plants.

    Science.gov (United States)

    Wang, Man; Bi, Wentao; Huang, Xiaohua; Chen, David Da Yong

    2016-06-03

    A ball mill assisted mechanochemical extraction method was developed to extract compounds of natural product (NP) from plant using ionic liquid (IL). A small volume ball mill, also known as PastPrep(®) Homogenizer, which is often used for high-speed lysis of biological samples and for other applications, was used to dramatically increase the speed, completeness and reproducibility of the extraction process at room temperature to preserve the chemical integrity of the extracted compounds. In this study, tanshinones were selected as target compounds to evaluate the performance of this extraction method. Factors affecting the extraction efficiency, such as the duration, IL concentration and solid/liquid ratio were systematically optimized using the response surface methodology. Under the optimized conditions, the described method was more efficient and much faster than the conventional extraction methods such as methanol based ultrasound assisted extraction (UAE) and heat reflux extraction (HRE) that consumes a lot more organic solvent. In addition, the natural products of interest were enriched by anion metathesis of ionic liquids, combining extraction and preconcentration in the same process. The extractant was analyzed by HPLC and LC-MS. The reproducibility (RSD, n=5), correlation coefficient (r(2)) of the calibration curve, and the limit of detection, were determined to be in the range of 4.7-5.2%, 0.9992-0.9995, and 20-51ng/mL, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Efficacy and Safety of 120-W Thulium:Yttrium-Aluminum-Garnet Vapoenucleation of Prostates Compared with Holmium Laser Enucleation of Prostates for Benign Prostatic Hyperplasia

    Directory of Open Access Journals (Sweden)

    Kai Hong

    2015-01-01

    Full Text Available Background: This study compared the efficacy and safety between 120-W thulium:yttrium-aluminum-garnet (Tm:YAG vapoenucleation of prostates (ThuVEP and holmium laser enucleation of prostates (HoLEP for patients with lower urinary tract symptoms (LUTS due to benign prostatic hyperplasia (BPH. Methods: A retrospective analysis of 88 consecutive patients with symptomatic BPH was carried out, who underwent either 120-W ThuVEP or HoLEP nonrandomly. Patient demographics and peri-operative and 12-month follow-up data were analyzed with the International Prostate Symptom Score (IPSS, quality of life (QoL score, maximum flow rate (Qmax, postvoid residual urine volume (PVR, and rates of peri-operative and late complications. Results: The patients in each group showed no significant difference in preoperative parameters. Compared with the HoLEP group, patients in the 120-W ThuVEP group required significantly shorter time for laser enucleation (58.3 ± 12.8 min vs. 70.5 ± 22.3 min, P = 0.003, and resulted in a significant superiority in laser efficiency (resected prostate weight/laser enucleation time for 120-W Tm:YAG laser compared to holmium:YAG laser (0.69 ± 0.18 vs. 0.61 ± 0.19, P = 0.048. During 1, 6, and 12 months of follow-ups, the procedures did not demonstrate a significant difference in IPSS, QoL score, Qmax, or PVR (P > 0.05. Mean peri-operative decrease of hemoglobin in the HoLEP group was similar to the ThuVEP group (17.1 ± 12.0 g/L vs. 15.2 ± 10.1 g/L, P = 0.415. Early and late incidences of complications were low and did not differ significantly between the two groups of 120-W ThuVEP and HoLEP patients (P > 0.05. Conclusions: 120-W ThuVEP and HoLEP are potent, safe and efficient modalities of minimally invasive surgeries for patients with LUTS due to BPH. Compared with HoLEP, 120-W ThuVEP offers advantages of reduction of laser enucleation time and improvement of laser efficiency.

  19. Laser Prostatectomy: Holmium Laser Enucleation and Photoselective Laser Vaporization of the Prostate

    Science.gov (United States)

    Bostanci, Yakup; Kazzazi, Amir; Djavan, Bob

    2013-01-01

    Historically, transurethral resection of the prostate has been the gold standard for the treatment of benign prostatic hyperplasia (BPH). Laser technology has been used to treat BPH for > 15 years. Over the past decade, it has gained wide acceptance by experienced urologists. This review provides an evidence-based update on laser surgery for BPH with a focus on photoselective laser vaporization and holmium laser enucleation of the prostate surgeries and assesses the safety, efficacy, and durability of these techniques. PMID:23671400

  20. Holmium laser enucleation of the prostate: a review of the clinical trial evidence

    Science.gov (United States)

    2014-01-01

    Transurethral resection of the prostate (TURP) has remained the procedure of choice for the surgical treatment of bladder outflow obstruction for almost five decades, but holmium laser enucleation of the prostate (HoLEP) is now emerging as a challenger as the gold standard procedure. This review summarizes the evidence base for HoLEP, with particular reference to randomized, controlled (level 1) evidence. PMID:24688602

  1. Giant Hepatic Hemangioma With Kasabach–Merritt Syndrome: Is the Appropriate Treatment Enucleation or Liver Transplantation?

    OpenAIRE

    Hochwald, Steven N.; Blumgart, Leslie H

    2000-01-01

    We present a case of giant cavernous hemangioma of the liver with disseminated intravascular coagulopathy (Kasabach–Merritt syndrome) which was cured by enucleation. The 51 year old woman presented with increased abdominal girth and easy bruisability. Workup elsewhere revealed a massive hepatic hemangioma and she was started on radiation therapy to the lesion and offered an orthotopic liver transplant. After careful preoperative preparation, we felt that resection w...

  2. Spontaneous alignment of permanent successors after enucleation of odontogenic cysts associated with primary teeth.

    Science.gov (United States)

    Chiu, W K; Sham, A S K; Hung, J N M

    2008-01-01

    Pulpotomy and pulpectomy occasionally lead to cyst formation in the primary dentition. They show specific clinical features of large size, rapid growth, buccal expansion and displacement of permanent teeth. Complete enucleation of the cyst with extraction of the associated primary teeth and preservation of the permanent teeth appeared to be the most suitable treatment option. Normal alignment of the permanent teeth occurred spontaneously even their initial positions were highly unfavourable.

  3. Digital mammography: computer-assisted diagnosis method for mass detection with multiorientation and multiresolution wavelet transforms.

    Science.gov (United States)

    Li, L; Qian, W; Clarke, L P

    1997-11-01

    The authors evaluated a modular computer-assisted diagnosis (CAD) method for mass detection that uses computation of features in three domains (gray level, morphology, and directional texture). Their objectives were to improve the sensitivity of detection and reduce the false-positive (FP) detection rate. The directional wavelet transform (DWT) method, which uses both multiorientation and multiresolution wavelet transforms to improve image preprocessing and segmentation of suspicious areas and to extract both morphologic and directional texture features, was evaluated with a previously reported image database containing 50 normal and 45 abnormal digitized screen-film mammograms. The mammograms contained all mass types and included 16 minimal cancers. This method was compared with the Markov random field (MRF) method to avoid issues related to case selection criteria. Free-response receiver operating characteristic curves were compared for both DWT and MRF methods. For the DWT method, the sensitivity was 98% and the FP detection rate was 1.8 FP findings per image. For the MRF method, the sensitivity was 90% and the FP detection rate was 2.0 FP findings per image. The CAD method applied to the full mammographic image is automatic and independent of mass type. The segmentation of masses as performed with this method may potentially allow visual interpretation according to American College of Radiology criteria.

  4. Le Fort I osteotomy to enucleation of grand proportions fissural cyst-presentation of case report.

    Science.gov (United States)

    Cavalcante, Rafael Correia; Durski, Fernanda; Deliberador, Tatiana Miranda; Giovanini, Allan Fernando; Rebellato, Nelson Luís Barbosa; da Costa, Delson João; Klüppel, Leandro Eduardo; Scariot, Rafaela

    2016-01-01

    Fissural cysts (FC) are caused by entraped epithelium between nasal and maxilar processes. They are commonly treated with surgical enucleation precedded or not by marsupialization depending on the cyst size. Biopsy of lesion is recommended due to confirm radiographic evaluation. It is rare to observe Le Fort I surgical approach to this type of injury. This study reports the case of an uncommon grand proportions fissural cyst in a female patient, 53, that was referred to the Oral and Maxillofacial Surgery Departament of Hospital XV presenting volume increase in maxilla associated with numbness of palate. Radiograph examination showed an intimate relationship between incisors apexes and FC. Expansion of both buccal and palate cortical was then confirmed as well as its unusual size, approximately 25 millimeters. Due to the abnormal size of lesion and possible impairment of upper incisors, LeFort I osteotomy associated with downfracture to cystic enucleation was the chosen treatment. After enucleation, the remaining space was filled with BIOSs and bioguide (lyophilized bone and collagen membrane). Patients' twelve months follow-up demonstrate no relapses and maintenance of teeth involved. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  5. A Case of Attempted Bilateral Self-Enucleation in a Patient with Bipolar Disorder.

    Science.gov (United States)

    Castro, Hannah Muniz; Alvarez, John; Bota, Robert G; Yonkers, Marc; Tao, Jeremiah

    2017-03-22

    Attempted and completed self-enucleation, or removal of one's own eyes, is a rare but devastating form of self-mutilation behavior. It is often associated with psychiatric disorders, particularly schizophrenia, substance induced psychosis, and bipolar disorder. We report a case of a patient with a history of bipolar disorder who gouged his eyes bilaterally as an attempt to self-enucleate himself. On presentation, the patient was manic with both psychotic features of hyperreligous delusions and command auditory hallucinations of God telling him to take his eyes out. On presentation, the patient had no light perception vision in both eyes and his exam displayed severe proptosis, extensive conjunctival lacerations, and visibly avulsed extraocular muscles on the right side. An emergency computed tomography scan of the orbits revealed small and irregular globes, air within the orbits, and intraocular hemorrhage. He was taken to the operating room for surgical repair of his injuries. Attempted and completed self-enucleation is most commonly associated with schizophrenia and substance induced psychosis, but can also present in patients with bipolar disorder. Other less commonly associated disorders include obsessive-compulsive disorder, depression, mental retardation, neurosyphilis, Lesch-Nyhan syndrome, and structural brain lesions.

  6. Technical aspects of transurethral plasmakinetic enucleation and resection of the prostate for benign prostatic hyperplasia.

    Science.gov (United States)

    Yu, Yang; Lou, Guantao; Shen, Chen; Guan, Sheng; Wang, Wei; Yang, Bo

    2017-02-01

    TUERP, which is based on TURP, technically has been proven to be safe and effective with reduced complication rates. Due to the lack of systematic pre-clinical training, the surgery is learnt only inside the operative theatre in the majority of the Chinese medical centers. It is also known to have a steep learning curve, and very few articles have addressed the technical aspects of TUERP. The videos of 91 cases of bipolar transurethral enucleation and resection of the prostate, which were performed by one urological surgeon in our department from August 2013 to January 2016, were retrospectively analyzed. With an extensive review of the literature and based on the summary of our experience, detailed techniques and tips for TUERP are described. The procedure is initiated from the enucleation of mid-lobe and progressed in a retrograde mode with the guide of the capsular plane. Along with the experience accumulated, the capability of landmark identifying and the surgical skills were improved. A steep operative learning curve may be the main obstacle to the widespread use of TUERP. Accurate identification and orientation of the landmark with good understanding of the three-dimensional structure of the prostatic fossa and the detailed technical issues of enucleating along the right capsule plane will be helpful for beginners to overcome the learning curve and gain confidence with this procedure and for experienced surgeons to further improve their surgical technique.

  7. Clinical Outcomes of Transurethral Enucleation with Bipolar for Benign Prostatic Hypertrophy.

    Science.gov (United States)

    Kawamura, Yoshiaki; Tokunaga, Masatoshi; Hoshino, Hideaki; Matsushita, Kazuo; Terachi, Toshiro

    2015-12-20

    This study compared outcomes of transurethral enucleation with bipolar (TUEB) with transurethral resection in saline (TURis). Thirty patients who underwent TURis were compared with 30 who underwent TUEB. Perioperative treatment outcomes, preoperative and 1-month postoperative International Prostrate Symptom Scores (IPSS), quality of life (QOL) index, maximum flow rate, average urinary flow, post- void residual urinary volume, and complications were compared. There were no significant differences in IPSS, measurements of urinary flow, or duration of catheterization. However, the improvement of QOL index after surgery was significantly greater in the TUEB group than the TURis group. The TUEB group had significantly longer surgical time, but tended to have greater enucleated tissue weight than the TURis group. There was no significant difference in enucleated tissue weight per unit time between the groups. The TUEB group also tended to have less hemoglobin decrease at postoperative day 1; this tendency was more prominent in patients with an estimated prostate volume of ≥ 50 ml. No significant differences in postoperative complications were observed. This study confirmed that the previously reported safety and efficacy of TUEB are comparable to those of TURis. TUEB appears especially safe for those with a large benign hypertrophic prostate.

  8. Transurethral enucleation of prostate with button electrode plasmakinetic vaporization for the treatment of Benign Prostatic Hyperplasia.

    Science.gov (United States)

    Peng, Bo; Huang, Jianhua; Wang, Guangchun; Zhang, Haimin; Liu, Min

    2016-12-23

    Benign prostatic hyperplasia (BPH) is a common disease in aged men. In this study, we investigated the efficacy and safety of transurethral enucleation of prostate with button electrode plasmakinetic vaporization for the treatment of BPH. 60 patients diagnosed with BPH who were treated in our hospital from August to December, 2014 by enucleation with button electrode were retrospectively reviewed, and operation time, urinary catheter indwelling time, continuous bladder irrigation time, operation related complications, maximum urinary flow rate (Qmax), post-void residual urine volume (PVR), International Prostate Symptom Score (IPSS), quality of life assessment (QOL), perioperative hemoglobin and electrolytes were recorded. All the operations were completed successfully. The operation time and urinary catheter indwelling time were 45.3 ± 16.2 min and 1.72 ± 0.32 d, respectively. During the follow-up, urethral stricture (n = 1), and urinary incontinence (n = 2) were found with recovery after 1-month training. Postoperative PVR at 1, 3 and 6 months significantly decreased compared with preoperative ones (P enucleation of prostate with button electrode was efficient and safe, which was worth being recommended.

  9. A case of attempted bilateral self-enucleation in a patient with bipolar disorder

    Directory of Open Access Journals (Sweden)

    Hannah Muniz Castro

    2017-06-01

    Full Text Available Attempted and completed self-enucleation, or removal of one’s own eyes, is a rare but devastating form of self-mutilation behavior. It is often associated with psychiatric disorders, particularly schizophrenia, substance induced psychosis, and bipolar disorder. We report a case of a patient with a history of bipolar disorder who gouged his eyes bilaterally as an attempt to self-enucleate himself. On presentation, the patient was manic with both psychotic features of hyperreligous delusions and command auditory hallucinations of God telling him to take his eyes out. On presentation, the patient had no light perception vision in both eyes and his exam displayed severe proptosis, extensive conjunctival lacerations, and visibly avulsed extraocular muscles on the right side. An emergency computed tomography scan of the orbits revealed small and irregular globes, air within the orbits, and intraocular hemorrhage. He was taken to the operating room for surgical repair of his injuries. Attempted and completed self-enucleation is most commonly associated with schizophrenia and substance induced psychosis, but can also present in patients with bipolar disorder. Other less commonly associated disorders include obsessive-compulsive disorder, depression, mental retardation, neurosyphilis, Lesch-Nyhan syndrome, and structural brain lesions.

  10. Clinical comparison of ultrasonic surgery and conventional surgical techniques for enucleating jaw cysts.

    Science.gov (United States)

    Yaman, Z; Suer, B T

    2013-11-01

    The conventional treatment of odontogenic cysts usually involves enucleation of the cyst using rotary and manual instruments; such procedures can cause trauma to the cystic epithelium or soft tissues in the region, such as sinus membrane perforation or nerve damage. The use of ultrasonic surgery may reduce the risk of damage to soft tissues. The objective of this study was to evaluate the performance of ultrasonic surgery in removing odontogenic cysts. Eighty-two cysts were removed from 68 patients over a period of 45 months. Ultrasonic surgery was used for 34 patients and conventional surgical procedures were used for 34 control patients. Two surgeons rated the cutting efficiency, visibility of the surgical field, ease of operation, and ease of cyst epithelium removal on a 100-mm visual analogue scale. The operation time was also recorded. No major intraoperative or postoperative complications were observed, and there was no cyst recurrence. Ultrasonic surgery for enucleating jaw cysts was found to increase the operation time, but also markedly increased the visibility of the operation field. In cases where cyst enucleation is performed in difficult areas that require delicate manipulation, there is less risk of damage to vital structures such as neurovascular tissues with ultrasonic surgery. Copyright © 2013 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  11. Microwave-assisted extraction of glucosinolates from Eruca sativa seeds and soil: comparison with existing methods.

    Science.gov (United States)

    Omirou, Michalis; Papastylianou, Ioannis; Iori, Renato; Papastephanou, Chara; Papadopoulou, Kalliope K; Ehaliotis, Costantinos; Karpouzas, Dimitrios G

    2009-01-01

    Glucosinolates (GSLs) are secondary plant metabolites that are abundant in brassicas and their hydrolysis products, isothiocyanates, are toxic to soil pathogens. Efficiency and extraction time are critical for routine analysis of GSLs in plant tissues. Robust analytical procedures are required for the extraction of GSL from soil. Development and optimisation of a microwave-assisted extraction (MAE) method for the recovery of GSL from plant tissues and soil and comparison of its efficiency with other established extraction methods. Solvents, temperature, microwave power and extraction time were examined as parameters controlling MAE efficiency. In rocket seeds the efficiency of MAE was determined through recovery of GSLs from seeds and of sinigrin (1) that was used as internal standard. MAE was then compared with the certified ISO-9167 method and an ultrasonic-assisted extraction (UAE). MAE was also applied for the extraction of GSLs from soils fortified with 1 at three fortification levels. The efficiency of MAE was compared with a recently proposed agitation-filtration (AGIT) extraction method. The optimum conditions identified for extraction of GSLs from seeds were: methanol extraction at 250 W and 80 degrees C for 10 min. MAE and ISO methods showed comparable efficiencies and higher than UAE. In soil, both methods resulted in nearly 100% recovery of 1 at all fortification levels, although MAE achieved this recovery after a single extraction step compared with AGIT, which required two. The MAE developed is a simple and rapid method for the extraction of GSLs from plant tissues and soil that can be applied to a large number of samples, thus reducing the time of analysis. Copyright (c) 2009 John Wiley & Sons, Ltd.

  12. Identifying strategies to assist final semester nursing students to develop numeracy skills: a mixed methods study.

    Science.gov (United States)

    Ramjan, Lucie M; Stewart, Lyn; Salamonson, Yenna; Morris, Maureen M; Armstrong, Lyn; Sanchez, Paula; Flannery, Liz

    2014-03-01

    It remains a grave concern that many nursing students within tertiary institutions continue to experience difficulties with achieving medication calculation competency. In addition, universities have a moral responsibility to prepare proficient clinicians for graduate practice. This requires risk management strategies to reduce adverse medication errors post registration. To identify strategies and potential predictors that may assist nurse academics to tailor their drug calculation teaching and assessment methods. This project builds on previous experience and explores students' perceptions of newly implemented interventions designed to increase confidence and competence in medication calculation. This mixed method study surveyed students (n=405) enrolled in their final semester of study at a large, metropolitan university in Sydney, Australia. Tailored, contextualised interventions included online practice quizzes, simulated medication calculation scenarios developed for clinical practice classes, contextualised 'pen and paper' tests, visually enhanced didactic remediation and 'hands-on' contextualised workshops. Surveys were administered to students to determine their perceptions of interventions and to identify whether these interventions assisted with calculation competence. Test scores were analysed using SPSS v. 20 for correlations between students' perceptions and actual performance. Qualitative open-ended survey questions were analysed manually and thematically. The study reinforced that nursing students preferred a 'hands-on,' contextualised approach to learning that was 'authentic' and aligned with clinical practice. Our interventions assisted with supporting students' learning and improvement of calculation confidence. Qualitative data provided further insight into students' awareness of their calculation errors and preferred learning styles. Some of the strongest predictors for numeracy skill performance included (1) being an international student, (2

  13. Enucleation of a large true cyst in the head of pancreas instead of Whipple procedure in an 8-year old boy: a case report

    Directory of Open Access Journals (Sweden)

    Ostadian N

    2011-02-01

    Full Text Available "nBackground: Simple cysts of pancreas are smaller than complex ones and are more likely to be located at the distal tail of the pancreas. Simple cysts are often asymptomatic and can be managed conservatively by observation. There seems to be few clinical trials to suggest the best treatments method for large symptomatic cysts of pancreas located at the head of the organ."n "nCase presentation: In this report, we describe an eight-year old boy with a large symptomatic true cyst at the head of the pancreas who was successfully treated by enucleation of the cyst, instead of the Whipple's procedure, without any ensuing complications."n "nConclusion: Regarding the rarity of simple cysts in the pancreas, lack of studies to compare different surgical procedures and suggest the best methods to treat them and the considerable morbidity and even mortality of major surgeries (e.g Whipple's procedure enucleation of these cysts seem to be appropriate for treating them with no early or late complications. More studies are needed to warrant the results of this report.

  14. New method of direct percutaneous endoscopic jejunostomy tube placement using balloon-assisted enteroscopy with fluoroscopy.

    Science.gov (United States)

    Velázquez-Aviña, Jacobo; Beyer, Ryan; Díaz-Tobar, Claudia P; Peter, Shajan; Kyanam Kabir Baig, Kondal R; Wilcox, C Mel; Mönkemüller, Klaus

    2015-03-01

    Direct percutaneous endoscopic jejunostomy (DPEJ) is a useful method to provide nutrition to patients with a variety of gastrointestinal (GI) problems. The present study describes a new method of DPEJ using balloon-assisted-enteroscopy. This observational, retrospective, single-arm case study conducted at a tertiary care hospital during a 15-month period included 25 patients (12 females, 13 males, mean age 54 years, age range 31-79 years) with necrotizing pancreatitis, n = 7; complex upper GI surgery, n = 6; complex fistula, n = 6; impossibility to place a gastrostomy tube, n = 5; and bowel obstruction, n = 1. The new DPEJ technique focused on three key components: (i) use of balloon-assisted overtube; (ii) use of fluoroscopy; (iii) leaving the overtube in place during the entire procedure (and also for DPEJ removal). Technical success was 96%. Mean time of the procedure was 30.5 min (range 24 to 45 min). Clinical success was 100% (24/24); all DPEJ could be used for their intended purpose. This new method of inserting a DPEJ using balloon enteroscopy and fluoroscopy was safe and successful. Future comparative studies are now warranted. © 2014 The Authors. Digestive Endoscopy © 2014 Japan Gastroenterological Endoscopy Society.

  15. Novel in situ method (vacuum assisted electroless plating) modified porous cathode for solid oxide fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Su, Ren; Lue, Zhe; Chen, Kongfa; Ai, Na; Li, Shuyan; Wei, Bo [Center for the Condensed Matter Science and Technology, Harbin Institute of Technology, Harbin 150001 (China); Su, Wenhui [Center for the Condensed Matter Science and Technology, Harbin Institute of Technology, Harbin 150001 (China); Department of Condensed Matter Physics, Jilin University, Changchun 130023 (China); International Centre for Materials Physics, Academia Sinica, Shenyang 110015 (China)

    2008-06-15

    A novel in situ method - vacuum assisted electroless plating (VA-EP) is developed to modify the porous structure of various materials. The advantage of this method is that it can form a metal network based on the already-given structure. We utilize this method to deposit silver (VA-EPA) in porous perovskite cathode Ba{sub 0.5}Sr{sub 0.5}Co{sub 0.8}Fe{sub 0.2}O{sub 3-{delta}} (BSCF) for an intermediate temperature solid oxide fuel cell (IT-SOFC) in the present research. The results of investigation show the performance of the modified cathode (VA-EPA-BSCF) enhances greatly, for example, the polarization resistance of VA-EPA-BSCF decreases by 60% at 600 C compared to BSCF. (author)

  16. Patterned growth of luminescent metal-organic framework films: a versatile electrochemically-assisted microwave deposition method.

    Science.gov (United States)

    Li, Wei-Jin; Feng, Ji-Fei; Lin, Zu-Jin; Yang, Ying-Long; Yang, Yan; Wang, Xu-Sheng; Gao, Shui-Ying; Cao, Rong

    2016-03-11

    Electrochemically-assisted microwave deposition technology, a facile method for the fabrication of luminescent metal-organic framework (LMOF) films, is presented herein. This method was further developed into a versatile method for preparing patterned LMOF films. The strategy based on this method can spatially locate microcrystals of MOFs on a surface, which provides great promise in anti-counterfeiting barcode applications.

  17. Prostate tissue retrieval after holmium laser enucleation of the prostate; assessment of non-morcellation approaches.

    Science.gov (United States)

    Elshal, Ahmed M; Eldemerdash, Yasser; Mekkawy, Ramy; Taha, Diaa-Eldin; Laymon, Mahmoud; El-Nahas, Ahmed R; El-Assmy, Ahmed

    2016-06-01

    To review non-morcellation approaches for tissue retrieval after holmium laser enucleation of the prostate (HoLEP) and whether these approaches demolish the advantages of the HoLEP procedure. We reviewed our prospectively maintained laser prostate database for HoLEP procedures where non-morcellation approaches were used for retrieval of the enucleated adenoma. Non-morcellation approaches were adopted in cases of morcellator malfunction or whenever concomitant pathology indicated laparotomy. Patients were stratified into the laparotomy group (Group I) or the transurethral resection (TUR) group (Group II). Safety and efficacy of each approach were assessed and compared. Between August 2012 and July 2015, of 392 HoLEP procedures non-morcellation approaches were used for tissue retrieval in 37 (9.4%). In 19 procedures a laparotomy approach was adopted (17 mini-laparotomies and two conventional laparotomies for concomitant diverticulectomy). TUR of the enucleated adenoma was adopted in 18 patients. Baseline demographic data and indications for surgery were comparable between the groups. However, significantly larger prostates were treated in Group I. There were no significant differences between the groups for tissue retrieval time, histopathological findings of retrieved tissue, and peri-procedure biochemical changes. However, significantly more tissue was retrieved (median tissue weight 115 vs 38 g) and at a faster rate (4.6 vs 1.09 g/min) in Group I. The median hospital stay was similar in both groups, but the median time to catheter removal was longer in Group I (5 vs 2 days). Minimal and similar peri-procedure complications were reported in both groups and in both groups there was a significant and comparable improvement in all urinary outcome measures. In the absence or malfunction of a tissue morcellator, or whenever concomitant pathology indicates laparotomy, non-morcellation tissue retrieval approaches are feasible options for endourologists practicing

  18. Potential Suitable Methods for Measuring the Effects of Animal-Assisted Activities and Therapy: a Review

    Directory of Open Access Journals (Sweden)

    Machová K.

    2016-09-01

    Full Text Available Dogs are companions of humans since time beyond memory and their use in rehabilitation processes is increasingly frequent. Dogs can be used for animal-assisted activities (AAA as well as animal-assisted therapy (AAT. The effects, however, have not been fully demonstrated, reasons for this including difficulties in providing evidence of positive action. According to previous studies, there is a decrease in heart and respiratory rate, blood pressure reduction, more positive mood as well as warming of muscles, which promotes relaxation of spastic areas. Regulation of hormone levels might also occur as a result of AAA/AAT. Indeed, increases in oxytocin levels and decreases of cortisol have been found. However, a unified methodology for clear measuring the entire impact of AAA/AAT on patients is missing. This survey evaluated different methods for measuring the effects of AAA/AAT, with results showing that the most suitable ones, selected on the basis of the effect of rehabilitation, comprise thermography, spectral analysis of heart rate, electromyography (EMG, polyelectromyography (PEMG, and blood sampling. Because AAT making use of dogs has not yet been recognized as an official method of treatment, it is very important to find out objective means to evidence its beneficial effects. Highlights:

  19. The safety and efficacy of front-firing green-light laser endoscopic en bloc photoselective vapo-enucleation of non-muscle-invasive bladder cancer

    Science.gov (United States)

    Cheng, Bo; Qiu, Xiaofu; Li, Huanhui; Yang, Guosheng

    2017-01-01

    Purpose Laser therapy provides an alternative option for treating non-muscle-invasive bladder cancer (NMIBC). However, the clinical evidence for potassium-titanyl-phosphate (KTP) laser en bloc resection is still limited. Here, we investigated the efficacy and safety of the 120-W front-firing KTP laser for the treatment of NMIBC. Methods A total of 64 patients with NMIBC treated with either a 120-W front-firing KTP-photoselective vapo-enucleation of the bladder tumor (PVEBT, n=34) or transurethral resection of the bladder tumor (TURBT, n=30) were included. En bloc resection was applied to the patients in PVEBT group. Results There was no significant difference in rinsing time (P=0.292), indwelling catheter (P=0.080), pathologic type, and T stage (P=0.870) between the two groups. Compared with the TURBT group, patients treated with PVEBT had a shorter hospitalization stay (P=0.044), a shorter operation time (P=0.008), and a lower muscle miss rate (P=0.044). PVEBT is superior to TURBT in terms of the rate of 1-year recurrence (P=0.015) and tumor grade progression rate (P=0.019). Conclusion The 120-W front-firing KTP laser en bloc enucleation technique is a safe and feasible procedure for treating patients with NMIBC. Further external validation in larger cohorts with a long follow-up period is warranted. PMID:28860782

  20. Development of microwave assisted spectrophotometric method for the determination of glucose.

    Science.gov (United States)

    Ali, Asif; Hussain, Zahid; Arain, Muhammad Balal; Shah, Nasrullah; Khan, Khalid Mohammad; Gulab, Hussain; Zada, Amir

    2016-01-15

    A spectrophotometric method was developed based on the microwave assisted synthesis of Maillard product. Various conditions of the reaction were optimized by varying the relative concentration of the reagents, operating temperature and volume of solutions used in the reaction in the microwave synthesizer. The absorbance of the microwave synthesized Maillard product was measured in the range of 360-740 nm using UV-Visible spectrophotometer. Based on the maximum absorbance, 370 nm was selected as the optimum wave length for further studies. The LOD and LOQ of glucose was found 3.08 μg mL(-1) and 9.33 μg mL(-1) with standard deviation of ±0.05. The developed method was also applicable to urine sample. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Development of microwave assisted spectrophotometric method for the determination of glucose

    Science.gov (United States)

    Ali, Asif; Hussain, Zahid; Arain, Muhammad Balal; Shah, Nasrullah; Khan, Khalid Mohammad; Gulab, Hussain; Zada, Amir

    2016-01-01

    A spectrophotometric method was developed based on the microwave assisted synthesis of Maillard product. Various conditions of the reaction were optimized by varying the relative concentration of the reagents, operating temperature and volume of solutions used in the reaction in the microwave synthesizer. The absorbance of the microwave synthesized Maillard product was measured in the range of 360-740 nm using UV-Visible spectrophotometer. Based on the maximum absorbance, 370 nm was selected as the optimum wave length for further studies. The LOD and LOQ of glucose was found 3.08 μg mL- 1 and 9.33 μg mL- 1 with standard deviation of ± 0.05. The developed method was also applicable to urine sample.

  2. Modeling of hydrogen-assisted cracking in iron crystal using a quasi-Newton method.

    Science.gov (United States)

    Telitchev, Igor Ye; Vinogradov, Oleg

    2008-07-01

    A Quasi-Newton method was applied in the context of a molecular statics approach to simulate the phenomenon of hydrogen embrittlement of an iron lattice. The atomic system is treated as a truss-type structure. The interatomic forces between the hydrogen-iron and the iron-iron atoms are defined by Morse and modified Morse potential functions, respectively. Two-dimensional hexagonal and 3D bcc crystal structures were subjected to tensile numerical tests. It was shown that the Inverse Broyden's Algorithm-a quasi-Newton method-provides a computationally efficient technique for modeling of the hydrogen-assisted cracking in iron crystal. Simulation results demonstrate that atoms of hydrogen placed near the crack tip produce a strong deformation and crack propagation effect in iron lattice, leading to a decrease in the residual strength of numerically tested samples.

  3. Electrosurgical enucleation versus bipolar transurethral resection for prostates larger than 70 ml: a prospective, randomized trial with 5-year followup.

    Science.gov (United States)

    Zhu, Lingfeng; Chen, Shushang; Yang, Shunliang; Wu, Meijing; Ge, Rong; Wu, Weizhen; Liao, Lianming; Tan, Jianming

    2013-04-01

    We compared the perioperative and postoperative characteristics of prostate PlasmaKinetic™ enucleation and bipolar transurethral resection for large volume benign prostatic hyperplasia. In this prospective, randomized, controlled trial 80 patients with benign prostatic hyperplasia and a prostate of larger than 70 ml were randomly assigned to prostate bipolar transurethral resection or PlasmaKinetic enucleation. Operative time, resected adenoma weight, changes in hemoglobin, catheterization time and postoperative hospital stay were recorded and compared. Patients were followed 1, 6, 12, 24, 36, 48 and 60 months after surgery. Greater resected prostate weight (mean ± SD 64.2 ± 19.0 vs 50.6 ± 20.0 gm, p = 0.03), less blood loss (mean 0.87 ± 0.42 vs 1.74 ± 0.63 gm, p enucleation group than in the resection group. The postoperative improvement in International Prostate Symptom Score, quality of life, maximal flow rate and post-void residual urine volume was similar in the 2 groups at 1, 6, 12 and 24 months but significantly better in the enucleation group at 36, 48 and 60 months. During the 5-year followup no patient in the enucleation group but 2 in the resection group experienced recurrence. For large volume benign prostatic hyperplasia PlasmaKinetic enucleation of the prostate is associated with less blood loss, shorter hospital stay and catheterization time than bipolar transurethral resection of the prostate. Moreover, PlasmaKinetic enucleation seems to be superior at long-term followup with fewer reoperations necessary. Copyright © 2013 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

  4. Strain differences of the effect of enucleation and anophthalmia on the size and growth of sensory cortices in mice.

    Science.gov (United States)

    Massé, Ian O; Guillemette, Sonia; Laramée, Marie-Eve; Bronchti, Gilles; Boire, Denis

    2014-11-07

    Anophthalmia is a condition in which the eye does not develop from the early embryonic period. Early blindness induces cross-modal plastic modifications in the brain such as auditory and haptic activations of the visual cortex and also leads to a greater solicitation of the somatosensory and auditory cortices. The visual cortex is activated by auditory stimuli in anophthalmic mice and activity is known to alter the growth pattern of the cerebral cortex. The size of the primary visual, auditory and somatosensory cortices and of the corresponding specific sensory thalamic nuclei were measured in intact and enucleated C57Bl/6J mice and in ZRDCT anophthalmic mice (ZRDCT/An) to evaluate the contribution of cross-modal activity on the growth of the cerebral cortex. In addition, the size of these structures were compared in intact, enucleated and anophthalmic fourth generation backcrossed hybrid C57Bl/6J×ZRDCT/An mice to parse out the effects of mouse strains and of the different visual deprivations. The visual cortex was smaller in the anophthalmic ZRDCT/An than in the intact and enucleated C57Bl/6J mice. Also the auditory cortex was larger and the somatosensory cortex smaller in the ZRDCT/An than in the intact and enucleated C57Bl/6J mice. The size differences of sensory cortices between the enucleated and anophthalmic mice were no longer present in the hybrid mice, showing specific genetic differences between C57Bl/6J and ZRDCT mice. The post natal size increase of the visual cortex was less in the enucleated than in the anophthalmic and intact hybrid mice. This suggests differences in the activity of the visual cortex between enucleated and anophthalmic mice and that early in-utero spontaneous neural activity in the visual system contributes to the shaping of functional properties of cortical networks. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Advanced Unilateral Retinoblastoma: The Impact of Ophthalmic Artery Chemosurgery on Enucleation Rate and Patient Survival at MSKCC.

    Directory of Open Access Journals (Sweden)

    David H Abramson

    Full Text Available To report on the influence of ophthalmic artery chemosurgery (OAC on enucleation rates, ocular and patient survival from metastasis and impact on practice patterns at Memorial Sloan Kettering for children with advanced intraocular unilateral retinoblastoma.Single-center retrospective review of all unilateral retinoblastoma patients with advanced intraocular retinoblastoma treated at MSKCC between our introduction of OAC (May 2006 and December 2014. End points were ocular survival, patient survival from metastases and enucleation rates.156 eyes of 156 retinoblastoma patients were included. Primary enucleation rates have progressively decreased from a rate of >95% before OAC to 66.7% in the first year of OAC use to the present rate of 7.4%. The percent of patients receiving OAC has progressively increased from 33.3% in 2006 to 92.6% in 2014. Overall, ocular survival was significantly better in eyes treated with OAC in the years 2010-2014 compared to 2006-2009 (p = 0.023, 92.7% vs 68.0% ocular survival at 48 months. There have been no metastatic deaths in the OAC group but two patients treated with primary enucleation have died of metastatic disease.OAC was introduced in 2006 and its impact on patient management is profound. Enucleation rates have decreased from over 95% to less than 10%. Our ocular survival rate has also significantly and progressively improved since May 2006. Despite treating more advanced eyes rather then enucleating them patient survival has not been compromised (there have been no metastatic deaths in the OAC group. In our institution, enucleation is no longer the most common treatment for advanced unilateral retinoblastoma.

  6. Modified exposure method for gastric mobilization in robot-assisted esophagectomy.

    Science.gov (United States)

    Zheng, Yu; Zhao, Xi-Wen; Zhang, Han-Lu; Wang, Zi-Hao; Wang, Yun

    2017-12-01

    We describe a modified method to facilitate gastric mobilization in robotic esophagectomy. Furthermore, we performed a prospective comparative analysis of surgical outcomes between the conventional method and described technique. From April 1st, 2016 to December 31st, 2016, 59 consecutive patients were included who underwent robot-assisted McKeown esophagectomy for esophageal squamous cell carcinoma in our institution. They were subdivided into two groups based on the method of gastric exposure: a grasper retraction (GR) group (n=27) and a thread retraction (TR) group (n=32). For the GR patients, robotic instruments were directly used to expose the surgical field for gastric mobilization. However, for TR patients, the right gastroepiploic arcade and the short gastric vessels were fully exposed via a polyester tape combined with a thread loop. There was no incidence of postoperative 30-day mortality. The median gastric mobilization time was 53 min (range, 38-77 min). It took significantly less time in the TR group compared to the GR group (P=0.005). The median amount of blood loss was 8 mL (range, 5-14 mL), and no significant difference was found between the two groups (P=0.573). The median number of dissected lymph nodes was 10 (range, 7-16), and there was no significant difference between groups (P=0.386). Similarly, the postoperative morbidity rates did not statistically differ between the two groups (P=0.942). The robot-assisted McKeown procedure presented is a safe and easy to perform technique for stomach retraction during gastric mobilization. Compared with the conventional GR method of gastric mobilization, TR requires less operating time and allows for an excellent operative field. The technique could, therefore, help surgeons to overcome some of the defects of robotic esophagectomy during gastric mobilization.

  7. Fatty Acid Characteristics of Isochrysis galbana Lipids Extracted Using a Microwave-Assisted Method

    Directory of Open Access Journals (Sweden)

    Cherng-Yuan Lin

    2015-02-01

    Full Text Available Lipids were extracted from Isochrysis galbana using a microwave-assisted method accompanied by various types of organic solvents. The effects of organic solvent type and microwave input energy on the fatty acid characteristics of the extracted lipids and their biodiesel product were investigated. Variations in the characteristics of the lipids extracted using a combination of n-hexane and iso-propanol solvents in both emulsion and direct mixtures were also compared. The experimental results showed that greater quantities of Isochrysis galbana lipids, and fatty acid methyl esters transesterified from those lipids, were extracted when using microwave irradiation with an organic solvent mixture of n-hexane and isopropanol in a 2:1 volumetric ratio than when using either n-hexane or isopropanol as the sole solvent. A greater quantity of Isochrysis galbana lipids was extracted when an emulsion of isopropanol solvent evenly dispersed in the continuous phase of n-hexane solvent was used than when a direct mixture of the two solvents was used. In addition, the quantity of lipids extracted from the dried Isochrysis galbana powder with the assistance of microwave irradiation was 9.08 wt% greater than when using traditional Soxhlet extraction without microwave irradiation.

  8. A computer-assisted systematic quality monitoring method for cervical hip fracture radiography

    Science.gov (United States)

    Laurin, Olof; Johnsson, Ragnar; Laurin, Sven

    2016-01-01

    Background A thorough quality analysis of radiologic performance is cumbersome. Instead, the prevalence of missed cervical hip fractures might be used as a quality indicator. Purpose To validate a computer-based quality study of cervical hip fracture radiography. Material and Methods True and false negative and positive hip trauma radiography during 6 years was assessed manually. Patients with two or more radiologic hip examinations before surgery were selected by computer analysis of the databases. The first of two preoperative examinations might constitute a missed fracture. These cases were reviewed. Results Out of 1621 cervical hip fractures, manual perusal found 51 (3.1%) false negative radiographic diagnoses. Among approximately 14,000 radiographic hip examinations, there were 27 (0.2%) false positive diagnoses. Fifty-seven percent of false negative reports were occult fractures, the other diagnostic mistakes. There were no significant differences over the years. Diagnostic sensitivity was 96.9% and specificity 99.8%. Computer-assisted analysis with a time interval of at least 120 days between the first and the second radiographic examination discovered 39 of the 51 false negative reports. Conclusion Cervical hip trauma radiography has high sensitivity and specificity. With computer-assisted analysis, 76% of false negative reports were found. PMID:27994880

  9. Chromosomes in the porcine first polar body possess competence of second meiotic division within enucleated MII stage oocytes.

    Directory of Open Access Journals (Sweden)

    Tao Lin

    Full Text Available To determine whether chromosomes in the porcine first polar body (PB1 can complete the second meiotic division and subsequently undergo normal pre-implantation embryonic development, we examined the developmental competence of PB1 chromosomes injected into enucleated MII stage oocytes by nuclear transfer method (chromosome replacement group, CR group. After parthenogenetic activation (PA or in vitro fertilization (IVF, the cleavage rate of reconstructed oocytes in the IVF group (CR-IVF group, 36.4 ± 3.2% and PA group (CR-PA group, 50.8 ± 4.2% were significantly lower than that of control groups in which normal MII oocytes were subjected to IVF (MII-IVF group, 75.8 ± 1.5% and PA (MII-PA group, 86.9 ± 3.7%. Unfertilized rates was significantly higher in the CR-IVF group (48.6 ± 3.3% than in the MII-IVF group (13.1 ± 3.4%. The blastocyst formation rate was 8.3 ± 1.9% in the CR-PA group, whereas no blastocyst formation was observed in the CR-IVF group. To produce tetraploid parthenogenetic embryos, intact MII stage oocytes injected with PB1 chromosomes were electrically stimulated, treated with 7.5 μg/mL cytochalasin B for 3 h (MII oocyte + PB1 + CB group, and then cultured without cytochalasin B. The average cleavage rate of reconstructed oocytes was 72.5% (48 of 66, and the blastocyst formation rate was 18.7% (9 of 48. Chromosome analysis showed similar proportions of haploid and diploid cells in the control (normal MII oocytes and CR groups after PA; overall, 23.6% of blastocysts were tetraploid in the MII oocyte + PB1 + CB group. These results demonstrate that chromosomes in PB1 can participate in normal pre-implantation embryonic development when injected into enucleated MII stage oocytes, and that tetraploid PA blastocysts are produced (although at a low proportion when PB1 chromosomes are injected into intact MII stage oocytes.

  10. Tarp-Assisted Cooling as a Method of Whole-Body Cooling in Hyperthermic Individuals.

    Science.gov (United States)

    Hosokawa, Yuri; Adams, William M; Belval, Luke N; Vandermark, Lesley W; Casa, Douglas J

    2017-03-01

    We investigated the efficacy of tarp-assisted cooling as a body cooling modality. Participants exercised on a motorized treadmill in hot conditions (ambient temperature 39.5°C [103.1°F], SD 3.1°C [5.58°F]; relative humidity 38.1% [SD 6.7%]) until they reached exercise-induced hyperthermia. After exercise, participants were cooled with either partial immersion using a tarp-assisted cooling method (water temperature 9.20°C [48.56°F], SD 2.81°C [5.06°F]) or passive cooling in a climatic chamber. There were no differences in exercise duration (mean difference=0.10 minutes; 95% CI -5.98 to 6.17 minutes or end exercise rectal temperature (mean difference=0.10°C [0.18°F]; 95% CI -0.05°C to 0.25°C [-0.09°F to 0.45°F] between tarp-assisted cooling (48.47 minutes [SD 8.27 minutes]; rectal temperature 39.73°C [103.51°F], SD 0.27°C [0.49°F]) and passive cooling (48.37 minutes [SD 7.10 minutes]; 39.63°C [103.33°F], SD 0.40°C [0.72°F]). Cooling time to rectal temperature 38.25°C (100.85°F) was significantly faster in tarp-assisted cooling (10.30 minutes [SD 1.33 minutes]) than passive cooling (42.78 [SD 5.87 minutes]). Cooling rates for tarp-assisted cooling and passive cooling were 0.17°C/min (0.31°F/min), SD 0.07°C/min (0.13°F/min) and 0.04°C/min (0.07°F/min), SD 0.01°C/min (0.02°F/min), respectively (mean difference=0.13°C [0.23°F]; 95% CI 0.09°C to 0.17°C [0.16°F to 0.31°F]. No sex differences were observed in tarp-assisted cooling rates (men 0.17°C/min [0.31°F/min], SD 0.07°C/min [0.13°F/min]; women 0.16°C/min [0.29°F/min], SD 0.07°C/min [0.13°F/min]; mean difference=0.02°C/min [0.04°F/min]; 95% CI -0.06°C/min to 0.10°C/min [-0.11°F/min to 0.18°F/min]). Women (0.04°C/min [0.07°F/min], SD 0.01°C/min [0.02°F/min]) had greater cooling rates than men (0.03°C/min [0.05°F/min], SD 0.01°C/min [0.02°F/min]) in passive cooling, with negligible clinical effect (mean difference=0.01°C/min [0.02°F/min]; 95% CI 0.001

  11. Ultrasound-assisted extraction method for the simultaneous determination of emerging contaminants in freshwater sediments.

    Science.gov (United States)

    de Sousa, Diana Nara Ribeiro; Grosseli, Guilherme Martins; Mozeto, Antonio Aparecido; Carneiro, Renato Lajarim; Fadini, Pedro Sergio

    2015-10-01

    Sediments are the fate of several emerging organic contaminants, such as pharmaceuticals, personal care products and hormones, and therefore an important subject in environmental monitoring studies. In the present work, a simple and sensitive method was developed, validated and applied for the simultaneous extraction of atenolol, caffeine, carbamazepine, diclofenac, ibuprofen, naproxen, propranolol, triclosan, estrone, 17-β-estradiol and 17-α-ethinylestradiol using ultrasound-assisted extraction from freshwater sediment samples followed by solid-phase extraction clean-up and liquid chromatography with tandem mass spectrometry detection. The solvent type and extraction pH were evaluated to obtain the highest recoveries of the compounds. The best method shows absolute recoveries between 54.0 and 94.4% at 50 ng/g concentration. The method exhibits good precision with relative standard deviation ranging from 1.0-16%. The detection and quantification limits ranged from 0.006-0.067 and 0.016-0.336 ng/g, respectively. The developed method was successfully applied to freshwater sediment samples collected from different sites in Jundiaí River basin of São Paulo State, Brazil. The compounds atenolol, caffeine, propranolol and triclosan were detected in all the sampling sites with concentrations of 13.8, 41.0, 28.5 and 176 ng/g, respectively. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Relay-Assisted Partial Packet Recovery with IDMA Method in CDMA Wireless Network

    CERN Document Server

    Luo, Zhifeng; Wong, Albert Kai-sun; Qiu, Shuisheng

    2010-01-01

    Automatic Repeat Request (ARQ) is an effective technique for reliable transmission of packets in wireless networks. In ARQ, however, only a few erroneous bits in a packet will cause the entire packet to be discarded at the receiver. In this case, it's wasteful to retransmit the correct bit in the received packet. The partial packet recovery only retransmits the unreliable decoded bits in order to increase the throughput of network. In addition, the cooperative transmission based on Interleave-division multiple-access (IDMA) can obtain diversity gains with multiple relays with different locations for multiple sources simultaneously. By exploring the diversity from the channel between relay and destination, we propose a relay-assisted partial packet recovery in CDMA wireless network to improve the performance of throughput. In the proposed scheme, asynchronous IDMA iterative chip-by-chip multiuser detection is utilized as a method of multiple partial recovery, which can be a complementarity in a current CDMA ne...

  13. Surfactant-assisted synthesis of BaTiO3 nanoparticles by micro-emulsion method

    Directory of Open Access Journals (Sweden)

    J. L. Gao

    2015-10-01

    Full Text Available Barium titanate (BaTiO3 nanoparticles were successfully synthesized by using a surfactant-assisted method. The various processing parameters, namely, th e species of surfactant, reaction temperature and micro-emusion concentration had been varied, and the effects on the micrographs and crystal structure of BaTiO3 particles had been analyzed by scanning electron microscope (SEM, transmission electron microscopy (TEM and X-ray diffraction (XRD. XRD analyses confirming the tetragonal structure of the BaTiO3 nanoparticles using hexadecyl trimethyl ammonium Bromide (CTAB or nonylphenol polyoxyethylene ether (NP-10 as surfactant. The SEM analysis showed that by changing the species of surfactant, grains with different dimensions could be synthesized. TEM analyses indicate that BaTiO3 nanoparticles with 15–20 nm in diameter were successfully synthesized.

  14. Preliminary Study on Synthesis of Composite rGO/Ni by Microwave Assisted Method

    Science.gov (United States)

    Fakhri, Hafizh A.; Husnah, Miftahul; Hasdi Aimon, Akfiny; Ferry, Iskandar

    2017-07-01

    Composite reduced graphene oxide-nickel (rGO/Ni) was successfully synthesized by wet chemical process via microwave assisted method. The nickel fraction was varied 0%, 5% and 20%. Crystallinity, morphology, chemical composition, chemical bonding, and conductivity were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), elemental dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), 4 point probes and electrochemical impedance spectroscopy (EIS). Based on the XRD patterns, the crystal sizes were 1.62 nm for 5% rGO/Ni and 1.64 nm for 20% rGO/Ni respectively. Furthermore, vibration of C=C aromatic stretching was detected in the FTIR spectra, which corresponds to rGO fingerprint. These results give a new perspective on synthesis of composite rGO/Ni.

  15. Porous microspheres of manganese-cerium mixed oxides by a polyvinylpyrrolidone assisted solvothermal method

    Science.gov (United States)

    Schmit, F.; Bois, L.; Chiriac, R.; Toche, F.; Chassagneux, F.; Descorme, C.; Besson, M.; Khrouz, L.

    2017-04-01

    Mixed cerium manganese oxides were synthesized using a polyvinylpyrrolidone (PVP) assisted solvothermal method. Materials obtained after calcination at 400 °C were characterized by X-ray diffraction, scanning and transmission electron microscopies, electron paramagnetic resonance (EPR), Raman spectroscopy, thermal analysis and nitrogen adsorption/desorption isotherms. The influence of the synthesis parameters on the oxide structure, such as the Mn:Ce ratio or the amount of PVP, was discussed. Micrometric spheres of mixed Mn-Ce oxides, resulting from the aggregation of 100 nm porous snowflakes, were successfully synthesized. These snowflakes were formed from the aggregation of smaller oriented crystallites (size 4 nm). The hydrothermal stability of these materials was also investigated.

  16. EXTRACTION OF PHENOLIC COMPOUNDS FROM PETAI LEAVES (PARKIA SPECIOSA HASSK. USING MICROWAVE AND ULTRASOUND ASSISTED METHODS

    Directory of Open Access Journals (Sweden)

    Buanasari Buanasari

    2017-06-01

    Full Text Available The antioxidant has an activity to neutralize free radical compound that the body needs to avoid damage cells and tissues. Phenolic is one of the compounds that have an antioxidant activity. The influences of ultrasonic-assisted extraction (UAE and microwave-assisted extraction (MAE conditions on phenolic compounds of Parkia speciosa Hassk. leaves were investigated. The effects of temperature (40°C, 50°C, 60°C and 70°C, time (10, 30 and 50 minutes and material-solvent ratio (1:10, 1:13, 1:15 were evaluated based on the yield, total phenolic content (TPC and antioxidant activity. The result showed that the highest yield (15.82% was obtained at 1:15 (w/w of material-solvent ratio, 50°C of temperature and 50 minutes of extraction time for MAE. The highest yield of UAE is 15.53% that sample was obtained at 1:13 (w/w of material-solvent ratio, 60°C of optimal temperature and 30 minutes extraction time. The highest IC50 of UAE method extract was 52.55 ppm, while the extract obtained using MAE method was 50.44 ppm. UAE is more stable at higher temperatures. Time and solvent which was used more efficient than MAE. Extract of petai leaves (Parkia speciosa Hassk. were very potential to be used as a source of natural antioxidants because they have IC50 values from 41.39 to 66.00 ppm. Its antioxidants capacity is ranged from strong to very strong capacity.

  17. Does Surgeon Experience Affect Operative Time, Adverse Events and Continence Outcomes in Holmium Laser Enucleation of the Prostate? A Review of More Than 1,000 Cases.

    Science.gov (United States)

    Shigemura, Katsumi; Yamamichi, Fukashi; Kitagawa, Koichi; Yamashita, Masuo; Oka, Yasuhiko; Tanaka, Hirokazu; Fujisawa, Masato

    2017-09-01

    Holmium laser enucleation of the prostate has become an increasingly common surgical therapy for benign prostatic hyperplasia. However, the relationship between surgeon experience and surgical outcomes has not yet been fully investigated. In this study we investigated how surgeon experience with holmium laser enucleation of the prostate affected operative time, adverse events and outcomes related to urination. We gathered a total of 1,113 cases of holmium laser enucleation of the prostate from 5 hospitals in Hyogo Prefecture, Japan. Included were data on surgeon experience with the procedure, operative time, enucleation time, morcellation time, patient age, perioperative and postoperative surgery related complications, and outcomes related to urination. A total of 39 surgeons were included in analysis. Statistical data showed that increasing surgical experience significantly contributed only to surgical time, enucleation time and urinary incontinence after holmium laser enucleation (p = 0.0146, 0.0216 and 0.0405, respectively). No significant changes were seen postoperatively in surgery related factors such as morcellation time, resected prostate volume, infectious or noninfectious surgery related complications, or urination related outcomes (p >0.05) Experience with at least 20 cases in particular affected surgical time (p = 0.0050), enucleation time (p = 0.0068) and urinary incontinence after holmium laser enucleation (p = 0.0021). Surgeon experience contributed to shortened operative time and enucleation time, and to decreased postoperative urinary incontinence but not to surgery related complications or urination related outcomes as shown by maximum urine flow and post-void residual urine volume. We also found that experienced surgeons with 31 to 50 cases might be associated with complications after holmium laser enucleation in larger prostate cases. Based on these data further prospective studies are scheduled to establish a program for training in holmium

  18. [Comparison of holmium and thulium laser in transurethral enucleation of the prostate].

    Science.gov (United States)

    Shao, Qiang; Zhang, Feng-Bo; Shang, Dong-Hao; Tian, Ye

    2009-04-01

    To investigate and compare the effect and safety of Holmium laser and Thulium laser in transurethral enucleation of the prostate in BPH patients. Ninety-eight BPH patients were divided into 2 groups and underwent transurethral enucleation of the prostate with holmium laser (Ho group) and thulium laser (Th group) respectively. Comparisons were made between the 2 groups in operation time, bleeding volume, electrolyte, IPSS score, PVR and Qmax. No statistically significant differences were noted between the 2 groups in age and preoperative prostate volume, IPSS, PVR and Qmax (P > 0.05). The mean operation time was shorter in the Th group ([84.6 +/- 10.2] min) than in the Ho group ([70.5 +/- 7.5] min) (P = 0.032); blood loss was less in the former ([126.5 +/- 14.6] ml) than in the latter ([176.5 +/- 14.1] ml) (P = 0.071), with no blood transfusion necessitated; and the mean times of catheter indwelling were 2.4 d and 2.5 d respectively. There were no significant differences in the levels of hemoglobin and electrolyte before and after operation between the two groups, and no TURP syndrome was observed. IPSS, PVR and Qmax before operation were significantly different from those obtained 3 months after it (P 0.05). Both holmium and thulium laser transurethral enucleation of the prostate can alleviate LUTS in BPH patients with similar short-time effectiveness. Thulium is superior to holmium laser in hemostasis, but inferior to it in anatomical distinctness.

  19. Worldwide enucleation techniques and materials for treatment of retinoblastoma: an international survey.

    Science.gov (United States)

    Mourits, Daphne L; Hartong, Dyonne T; Bosscha, Machteld I; Kloos, Roel J H M; Moll, Annette C

    2015-01-01

    To investigate the current practice of enucleation with or without orbital implant for retinoblastoma in countries across the world. A digital survey identifying operation techniques and material used for orbital implants after enucleation in patients with retinoblastoma. We received a response of 58 surgeons in 32 different countries. A primary artificial implant is routinely inserted by 42 (72.4%) surgeons. Ten (17.2%) surgeons leave the socket empty, three (5.2%) decide per case. Other surgeons insert a dermis fat graft as a standard primary implant (n=1), or fill the socket in a standard secondary procedure (n=2; one uses dermis fat grafts and one artificial implants). The choice for porous implants was more frequent than for non-porous implants: 27 (58.7%) and 15 (32.6%), respectively. Both porous and non-porous implant types are used by 4 (8.7%) surgeons. Twenty-five surgeons (54.3%) insert bare implants, 11 (23.9%) use separate wrappings, eight (17.4%) use implants with prefab wrapping and two insert implants with and without wrapping depending on type of implant. Attachment of the muscles to the wrapping or implant (at various locations) is done by 31 (53.4%) surgeons. Eleven (19.0%) use a myoconjunctival technique, nine (15.5%) suture the muscles to each other and seven (12.1%) do not reattach the muscles. Measures to improve volume are implant exchange at an older age (n=4), the use of Restylane SQ (n=1) and osmotic expanders (n=1). Pegging is done by two surgeons. No (worldwide) consensus exists about the use of material and techniques for enucleation for the treatment of retinoblastoma. Considerations for the use of different techniques are discussed.

  20. Worldwide enucleation techniques and materials for treatment of retinoblastoma: an international survey.

    Directory of Open Access Journals (Sweden)

    Daphne L Mourits

    Full Text Available To investigate the current practice of enucleation with or without orbital implant for retinoblastoma in countries across the world.A digital survey identifying operation techniques and material used for orbital implants after enucleation in patients with retinoblastoma.We received a response of 58 surgeons in 32 different countries. A primary artificial implant is routinely inserted by 42 (72.4% surgeons. Ten (17.2% surgeons leave the socket empty, three (5.2% decide per case. Other surgeons insert a dermis fat graft as a standard primary implant (n=1, or fill the socket in a standard secondary procedure (n=2; one uses dermis fat grafts and one artificial implants. The choice for porous implants was more frequent than for non-porous implants: 27 (58.7% and 15 (32.6%, respectively. Both porous and non-porous implant types are used by 4 (8.7% surgeons. Twenty-five surgeons (54.3% insert bare implants, 11 (23.9% use separate wrappings, eight (17.4% use implants with prefab wrapping and two insert implants with and without wrapping depending on type of implant. Attachment of the muscles to the wrapping or implant (at various locations is done by 31 (53.4% surgeons. Eleven (19.0% use a myoconjunctival technique, nine (15.5% suture the muscles to each other and seven (12.1% do not reattach the muscles. Measures to improve volume are implant exchange at an older age (n=4, the use of Restylane SQ (n=1 and osmotic expanders (n=1. Pegging is done by two surgeons.No (worldwide consensus exists about the use of material and techniques for enucleation for the treatment of retinoblastoma. Considerations for the use of different techniques are discussed.

  1. Technical Aspects of Holmium Laser Enucleation of the Prostate for Benign Prostatic Hyperplasia

    Science.gov (United States)

    Kim, Myong; Lee, Hahn-Ey

    2013-01-01

    Holmium laser enucleation of the prostate (HoLEP) is a minimally invasive procedure and a size-independent treatment for benign prostatic hyperplasia with excellent long-term surgical outcome. HoLEP has become an alternative to conventional transurethral resection of the prostate or open prostatectomy owing to its efficacy and safety. Although HoLEP is known to have a steep learning curve, very few articles have addressed the technical aspects of HoLEP. Herein, we described detailed techniques and tips for HoLEP as performed at Seoul National University Hospital in a step-by-step manner with extensive review of the literature. PMID:24044089

  2. Holmium Laser Enucleation of the Prostate and Iatrogenic Arteriovenous Fistula Treated by Superselective Arterial Embolization

    Directory of Open Access Journals (Sweden)

    Anastasios D. Asimakopoulos

    2016-01-01

    Full Text Available Iatrogenic pelvic pseudoaneurysm with concomitant arteriovenous fistula has been described as a rare and challenging complication, which may occur during transurethral resection of the prostate. We provide the first report of this complication after holmium laser enucleation of the prostate for the treatment of benign prostatic hyperplasia. The attempt to control the bleeding by conversion to open surgery and placement of haemostatic stitches into the prostatic fossa failed. Angiography with superselective arterial embolization proved to be a modern, quick, safe, and efficient treatment of this uncommon complication.

  3. [Transurethral enucleation of the prostate with the holmium: YAG laser system: how much power is necessary?].

    Science.gov (United States)

    Rassweiler, J; Roder, M; Schulze, M; Muschter, R

    2008-04-01

    To evaluate the efficacy and safety of an intermediate power (50 W) holmium:YAG laser system for transurethral enucleation of the prostate (TULP) with the use of a new mechanical morcellator. Our results are compared with the results of high-powered holmium laser enucleation (HoLEP) presented in the literature. From December 2003 to January 2008, 129 patients with benign prostatic hyperplasia were treated by TULP. In 45 cases (group A; 2.0, 12 Hz) we used a bipolar resectoscope (VISTA/ACMI) for morcellation, whereas after that morcellation was accomplished with a modified endoscopic shaver (Wolf). Thirty-nine patients were treated using 25 W (group B; 2.0 J, 12 Hz), and 45 patients were treated using 40 W (group C; 2.2 J 18 Hz), who were compared with 45 matched-pair patients who received transurethral resection of the prostate (TURP; group D). Finally, the literature on HoLEP was reviewed. Bipolar morcellation significantly prolonged the operating time (135 vs. 131 vs. 96 min). The morcellation speed averaged 2.8 (range 1.3-5.2) g/min with no complications. The resection speed (retrieval rate) of 40-W TULP was comparable to that for TURP (0.71 vs. 0.76 g/min). The transfusion rate was lower than for TURP (8% vs. 12%), with a smaller Hb difference (3.1 vs. 3.8 mg/dl). Catheter times (3.4 vs. 4.1 days) were similar; however, hospital stay was significantly shorter after TULP (5.2 vs. 6.8 days). The complication rate was significantly lower (6.6% vs. 13.3%). The efficacy of HoLEP significantly improved with introduction of the morcellator: Resection speed increased from 0.34-0.61 g/min to 0.48-0.82 g/min. HoLEP was better than 40-W TULP regarding transfusion rate (0-4% vs. 8%) and catheter time (1.1-1.5 vs. 3.4 days). Complications and functional results were similar. CONCLUSION[UBERSCHRIFT]: The intermediate-power 50-W holmium laser together with the new morcellator enable safe transurethral enucleation of the prostate. As with HoLEP, the procedure has a significant

  4. Enucleation following treatment with intravenous pentamidine for Acanthamoeba sclerokeratitis

    Directory of Open Access Journals (Sweden)

    Rebecca A Kuennen

    2010-09-01

    Full Text Available Rebecca A Kuennen, Reynell Harder Smith, Thomas F Mauger, Elson CraigDepartment of Ophthalmology, The Ohio State University, Columbus, Ohio, USAPurpose: To describe the course and outcome of treatment of advanced Acanthamoeba sclerokeratitis with intravenous pentamidine.Methods: A case of advanced Acanthamoeba sclerokeratitis was resistant to conventional therapy and was treated with intravenous pentamidine. The eye was later removed due to incapacitating pain.Results: The eye showed Acanthamoeba organisms within the cornea and evidence of acute and chronic inflammation throughout the remainder of the eye. The patient has survived without orbital recurrence for 2 years.Conclusions: This case demonstrates late inflammation with active Acanthameoba keratitis following systemic pentamidine therapy.Keywords: keratitis, scleritis

  5. An evaluation of microwave-assisted fusion and microwave-assisted acid digestion methods for determining elemental impurities in carbon nanostructures using inductively coupled plasma optical emission spectrometry

    KAUST Repository

    Patole, Shashikant P.

    2015-10-21

    It is common for as-prepared carbon nanotube (CNT) and graphene samples to contain remnants of the transition metals used to catalyze their growth; contamination may also leave other trace elemental impurities in the samples. Although a full quantification of impurities in as-prepared samples of carbon nanostructures is difficult, particularly when trace elements are intercalated or encapsulated within a protective layer of graphitic carbon, reliable information is essential for reasons such as quantifying the adulteration of physico-chemical properties of the materials and for evaluating environmental issues. Here, we introduce a microwave-based fusion method to degrade single- and double-walled CNTs and graphene nanoplatelets into a fusion flux thereby thoroughly leaching all metallic impurities. Subsequent dissolution of the fusion product in diluted hydrochloric and nitric acid allowed us to identify their trace elemental impurities using inductively coupled plasma optical emission spectrometry. Comparisons of the results from the proposed microwave-assisted fusion method against those of a more classical microwave-assisted acid digestion approach suggest complementarity between the two that ultimately could lead to a more reliable and less costly determination of trace elemental impurities in carbon nanostructured materials. Graphical abstract A method for the complete digestion of carbon nanostructures has been demonstrated. Photographs (on the left side) show zirconium crucibles containing SWCNTs with flux of Na2CO3 and K2CO3, before and after microwave fusion; (on the right side) the appearance of the final solutions containing dissolved samples, from microwave-assisted fusion and microwave-assisted acid digestion. These solutions were used for determining the trace elemental impurities by ICP‒OES.

  6. Analysis of hydrocarbon contamination with membrane-assisted solvent extraction: comparison of agitation and sonication methods.

    Science.gov (United States)

    Szekeres, Z; Eke, Zs; Rikker, T; Torkos, K

    2009-10-09

    Membrane-assisted solvent extraction (MASE) coupled to large volume injection was applied to the determination of (gasoline-type) hydrocarbon contamination in water samples. Hexane was used as acceptor phase. 50 microL extract was injected in the programmed temperature vaporizer injector using combined split-splitless evaporation. The extraction conditions were optimized both for MASE with agitation and for MASE with sonication. In the course of optimization the effect of extraction time, extraction temperature, agitation speed, solvent volume, pH, ionic strength and the addition of methanol were tested. Over 75% recovery was accomplished in the range of diesel oil hydrocarbons (n-C(9)-n-C(24)). The developed method was validated. Linearity, accuracy and precision were tested. The method showed excellent linearity between 1 and 1000 microgL(-1) for n-alkanes and between 0.05 and 50 mgL(-1) for gasoline. The method was tested with comprehensive GCxGC as well and found to be non-discriminative to all major compounds of diesel oil.

  7. Quicker method for assessing influences on teaching assistant buy-in and practices in reformed courses

    Science.gov (United States)

    Wilcox, Matthew; Yang, Yuehai; Chini, Jacquelyn J.

    2016-12-01

    Teaching assistants (TAs) that lead reformed recitations and labs must understand and buy into the design of the course and the research-based instructional strategies that the course requires in order to create high-fidelity implementations. We present a model that outlines possible influences on TAs' buy-in and their in-class actions coupled with a method, using a Real-time Instructor Observation Tool-based [E. A. West et al. Phys. Rev. ST Phys. Educ. Res. 9, 010109 (2013)] exercise, to measure the effect of these influences that is not only quicker than interviews but also allows one to quantify these effects. We use this method to measure the influences on six graduate TAs teaching algebra-based introductory mechanics and electricity and magnetism recitations and labs ("mini studios") at the University of Central Florida. The results from the exercise are confirmed by interview responses from the TAs. We find a relatively high degree of buy-in to the design of the course, yet this is not reflected in the TAs' actions. The TAs' actions appear to be most influenced by student responses and expectations which do not align with the design of the course. Our study examines the effect of three influences shown in our model, and we argue that our method could be easily adapted to examine additional influences.

  8. Phantom-based evaluation method for surgical assistance devices in minimally invasive cochlear implantation

    Science.gov (United States)

    Lexow, G. Jakob; Kluge, Marcel; Majdani, Omid; Lenarz, Thomas; Rau, Thomas S.

    2017-03-01

    Several research groups have proposed individual solutions for surgical assistance devices to perform minimally invasive cochlear implantation. The main challenge is the drilling of a small bore hole from the surface of the skull to the inner ear at submillimetric accuracy. Each group tested the accuracy of their device in their respective test bench or in a small number of temporal bone specimens. This complicates the comparison of the different approaches. Thus, a simple and inexpensive phantom based evaluation method is proposed which resembles clinical conditions. The method is based on half-skull phantoms made of bone-substitute material - optionally equipped with an artificial skin replica to include skin incision within the evaluation procedure. Anatomical structures of the temporal bone derived from segmentations using clinical imaging data are registered into a computer tomographic scan of the skull phantom and used for the planning of the drill trajectory. Drilling is performed with the respective device under conditions close to the intraoperative setting. Evaluation of accuracy can either be performed through postoperative imaging or by means of added targets on the inside of the skull model. Two different targets are proposed: simple reference marks only for measuring the accuracy of the device and a target containing a scala tympani model for evaluation of the complete workflow including the insertion of the electrode carrier. Experiments using the presented method take place under reproducible conditions thus allowing the comparison of the different approaches. In addition, artificial phantoms are easier to obtain and handle than human specimens.

  9. miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1.

    Science.gov (United States)

    Zhang, Lingbo; Flygare, Johan; Wong, Piu; Lim, Bing; Lodish, Harvey F

    2011-01-15

    Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1.

  10. [Clinical efficiency of computer-assisted pedicle screw placement versus conventional method: a meta-analysis].

    Science.gov (United States)

    Xing, Dan; Ma, Xin-Long; Song, Dong-Hui; Ma, Jian-Xiong; Xu, Wei-Guo; Wang, Jie; Yang, Yang; Chen, Yang

    2012-10-01

    To compare the clinical efficacy of computer-assisted pedicle screw placement and conventional placement in the treatment of spinal disease. A systematic search of studies published between Jan. 1990 and Feb. 2012 was conducted using Medline, Embase, OVID, ScienceDirect and Cochrane Review databases. Randomized controlled trials (RCTs) and prospective controlled trials of comparing computer-assisted pedicle screw placement to conventional method performed at one center or multi-centers providing data on accuracy of placement and clinical effects were identified. Two study authors independently reviewed the 93 articles originally identified and selected 10 for analysis. Study title,demographic characteristics,number of pedicle screw,anatomical area and outcomes were extracted manually from all selected studies. RevMan 5.1 software was used for meta-analysis. Ten studies encompassing 2813 pedicle screws met the inclusion criteria. Overall,the result of meta-analysis indicated that there were significant differences between two groups in accuracy in placement of pedicle screw [OR = 2.58, 95% CI (1.18, 5.63)], insertion time [WMD = -2.15, 95% CI (-2.36, -1.94)]. However, there was only one study reported preparation time of pedicle screw of navigation group was longer than conventional group. No neurological complication in navigation group was reported. As a safety supplementary measure, computer navigation provide better accuracy in placement of pedicle screw and insertion time. The preparation time of pedicle screw may prolong due to the complexity of navigation system. Further reseach should include randomized controlled trials with well-planned methodology to limit bias.

  11. Benign Prostatic Hyperplasia Treatment by Transurethral Enucleation of the Prostate Using a 2-μm Laser

    OpenAIRE

    He-qing GUO; Zhou, Gao-Biao; Liu, Hong-Ming; Sun, Bin; Pan, Guang-Xin; Mu, Da-Wei; Yan, Jing-Ming; Xing, Ji-Zhang; Li, Di; Hong, Quan

    2015-01-01

    This study investigates the efficacy of benign prostatic hyperplasia (BPH) treatment by prostate transurethral enucleation using a 2-μm laser. A total of 107 patients with BPH were treated by prostate transurethral enucleation using a RevoLix 2-μm laser surgery system. Bleeding volume, operation time, catheterization time, voiding situation, maximum urinary flow rate, and hospital stay were observed. The mean operation time was 74 min ± 12 min (range 45 to 150 min), the mean follow-up period ...

  12. Water-assisted growth of graphene on carbon nanotubes by the chemical vapor deposition method.

    Science.gov (United States)

    Feng, Jian-Min; Dai, Ye-Jing

    2013-05-21

    Combining carbon nanotubes (CNTs) with graphene has been proved to be a feasible method for improving the performance of graphene for some practical applications. This paper reports a water-assisted route to grow graphene on CNTs from ferrocene and thiophene dissolved in ethanol by the chemical vapor deposition method in an argon flow. A double injection technique was used to separately inject ethanol solution and water for the preparation of graphene/CNTs. First, CNTs were prepared from ethanol solution and water. The injection of ethanol solution was suspended and water alone was injected into the reactor to etch the CNTs. Thereafter, ethanol solution was injected along with water, which is the key factor in obtaining graphene/CNTs. Transmission electron microscopy, scanning electron microscopy, X-ray diffraction, and Raman scattering analyses confirmed that the products were the hybrid materials of graphene/CNTs. X-ray photo-electron spectroscopy analysis showed the presence of oxygen rich functional groups on the surface of the graphene/CNTs. Given the activity of the graphene/CNT surface, CdS quantum dots adhered onto it uniformly through simple mechanical mixing.

  13. Synthesis and Raman analysis of SnS nanoparticles synthesized by PVP assisted polyol method

    Energy Technology Data Exchange (ETDEWEB)

    Baby, Benjamin Hudson; Mohan, D. Bharathi, E-mail: d.bharathimohan@gmail.com [Department of Physics, School of Physical, Chemical and Applied Sciences, Pondicherry University, R.V. Nagar, Kalapet, Puducherry-605014 (India)

    2015-06-24

    SnS film was prepared by a simple drop casting method after synthesizing SnS nanoparticles by using PVP assisted polyol method. Confocal Raman study was carried out for the as deposited and annealed (150, 300 and 400 °C) films at two different excitation wavelengths 514 and 785 nm. At the excitation wavelength of 514 nm, the Raman modes showed for a mixed phase of SnS and SnS{sub 2} up to 150 °C and then only a pure SnS phase was observed up to 400 °C due to the dissociation of SnS{sub 2} in to SnS by releasing S. The increase in intensity of Raman (A{sub g} and B{sub 3g}) as well as IR (B{sub 3u}) active modes of SnS are observed with increasing annealing temperature at excitation wavelength 785 nm due to the increased crystallinity and inactiveness of SnS{sub 2} modes. X-ray diffraction confirming the formation of a single phase of SnS while the greater homogeneity in both size and shape of SnS nanoparticles were confirmed through surface morphology from SEM.

  14. The Asymmetric Cell Division Regulators Par3, Scribble and Pins/Gpsm2 Are Not Essential for Erythroid Development or Enucleation.

    Directory of Open Access Journals (Sweden)

    Christina B Wölwer

    Full Text Available Erythroid enucleation is the process by which the future red blood cell disposes of its nucleus prior to entering the blood stream. This key event during red blood cell development has been likened to an asymmetric cell division (ACD, by which the enucleating erythroblast divides into two very different daughter cells of alternate molecular composition, a nucleated cell that will be removed by associated macrophages, and the reticulocyte that will mature to the definitive erythrocyte. Here we investigated gene expression of members of the Par, Scribble and Pins/Gpsm2 asymmetric cell division complexes in erythroid cells, and functionally tested their role in erythroid enucleation in vivo and ex vivo. Despite their roles in regulating ACD in other contexts, we found that these polarity regulators are not essential for erythroid enucleation, nor for erythroid development in vivo. Together our results put into question a role for cell polarity and asymmetric cell division in erythroid enucleation.

  15. Hereditary Spherocytosis and Hereditary Elliptocytosis: Aberrant Protein Sorting during Erythroblast Enucleation

    Energy Technology Data Exchange (ETDEWEB)

    Salomao, Marcela; Chen, Ke; Villalobos, Jonathan; Mohandas, Narla; An, Xiuli; Chasis, Joel Anne

    2010-02-08

    During erythroblast enucleation, membrane proteins distribute between extruded nuclei and reticulocytes. In hereditary spherocytosis (HS) and hereditary elliptocytosis (HE), deficiencies of membrane proteins, in addition to those encoded by the mutant gene, occur. Elliptocytes, resulting from protein 4.1R gene mutations, lack not only 4.1R but also glycophorin C, which links the cytoskeleton and bilayer. In HS resulting from ankyrin-1 mutations, band 3, Rh-associated antigen, and glycophorin A are deficient. The current study was undertaken to explore whether aberrant protein sorting, during enucleation, creates these membrane-spanning protein deficiencies. We found that although glycophorin C sorts to reticulocytes normally, it distributes to nuclei in 4.1R-deficient HE cells. Further, glycophorin A and Rh-associated antigen, which normally partition predominantly to reticulocytes, distribute to both nuclei and reticulocytes in an ankyrin-1-deficient murine model of HS. We conclude that aberrant protein sorting is one mechanistic basis for protein deficiencies in HE and HS.

  16. Are Histological Findings of Thulium Laser Vapo-Enucleation Versus Transurethral Resection of the Prostate Comparable?

    Science.gov (United States)

    Carmignani, Luca; Macchi, Alberto; Ratti, Dario; Finkelberg, Elisabetta; Casellato, Stefano; Bozzini, Giorgio; Maruccia, Serena; Marenghi, Carlo; Picozzi, Stefano

    2015-09-01

    We investigated if an adequate histological diagnosis can be made from tissue after Thulium laser vapo-enucleation of the prostate (ThuVEP) and whether it is comparable to transurethral prostate resection (TURP) tissue findings in patients with symptomatic benign prostatic hyperplasia. We analyzed 350 ThuLEP and 100 matched TURP tissue specimens from patients who underwent one of the two procedures between January 2009 and June 2014. Thulium Laser Enucleation of Prostate (ThuVEP) was combined with mechanical morcellation of the resected lobe. Each histological specimen was reviewed by two pathologists. Preoperative prostate ultrasound volume, total serum prostatic specific antigen and postoperative tissue weight were evaluated. Microscopic histological diagnosis was assessed by standard histological techniques and immunohistochemical evaluation. Patients were comparable in terms of age and preoperative total serum prostate specific antigen. Incidental adenocarcinoma and high grade PIN of the prostate were diagnosed in a comparable percent of specimens in the 2 groups (2.5 % in the ThuVEP group versus 3 % in the TURP group). Tissue thermal artifacts induced by the Thulium laser are mostly due to coagulation as that of the conventional monopolar diathermy in TURP. Tissue quality was maintained in the ThuVEP histological specimens. Tissue maintain histological characteristics and proprieties without modification for successive immunoistochemical analysis. The pathologist ability to detect incidental prostate cancer and PIN was maintained even if there is a quoted of vaporized tissue.

  17. [Transurethral enucleation plus pneumo-cystostomy rotary cut for large benign prostatic hyperplasia].

    Science.gov (United States)

    Dong, Yan-Xin; Wu, Yang; Zeng, Rui; Yang, Jun-Chang; Gao, Xiao-Kang; Zhu, Ming-De; Huo, Shuang-Jin; Li, Dong; Niguti

    2014-06-01

    To investigate the feasibility, effectiveness and practicability of transurethral enucleation plus pneumocystostomy rotary cut (TUE + PCRC) for large benign prostatic hyperplasia (BPH). We performed TUE + PCRC for 26 BPH patients aged 62 - 85 years with the prostate volume of 80 - 165 ml. We conducted transurethral enucleation of the hyperplastic prostate glands and pushed them into the bladder, followed by bladder puncture for pneumo-cystostomy rotary cut. All the surgical procedures were successfully accomplished, with the mean surgical time of 41 (32 - 54) minutes and intraoperative blood loss < 60 ml in all the cases. Twenty-three of the patients were followed up for 2 - 8 months, which revealed no stricture of the urethra or any other severe complications. Compared with the preoperative baseline, significant improvement was achieved in the IPSS (6.5 +/- 2.2 vs 26.2 +/- 2.4), QOL (1.4 +/- 0.9 vs 4.6 +/- 1.2) and Qmax ([5.8 +/- 1.0 ] vs [19.6 +/- 2.8] ml/s) of the patients after surgery (P < 0.01). TUE + PCRC, with its advantages of short operation time and less severe complications, is a safe and effective approach to the management of large BPH.

  18. Bipolar Button Transurethral Enucleation of Prostate in Benign Prostate Hypertrophy Treatment: A New Surgical Technique.

    Science.gov (United States)

    Giulianelli, Roberto; Gentile, Barbara; Albanesi, Luca; Tariciotti, Paola; Mirabile, Gabriella

    2015-08-01

    To evaluate the safety and efficacy of transurethral bipolar enucleation with a button electrode (B-TUEP) for the treatment of bladder outlet obstruction due to benign prostatic hyperplasia. Between July 2011 and March 2012, a single surgeon performed 50 B-TUEP. Preoperative and postoperative assessments included prostate-specific antigen, International Prostate Symptoms Score (IPSS), International Index of Erectile Function-5 (IIEF-5), quality of life (QoL) index, uroflowmetry with postvoiding residual (PVR) urinary volume, and prostate volume measured by transrectal ultrasonography. Intraoperatively, we evaluated B-TUEP time (enucleation and resection time). Perioperatively, we evaluated hemoglobin dosage, bladder irrigation time, catheterization time, acute urinary retention events, length of stay, patient readmission, and any endoscopic retreatments. Three months after surgery, 82% of the patients presented a significant improvement in maximum urine flow (Qmax; P transurethral incision of the prostate. B-TUEP using the Gyrus PK system is a rapid and safety technique with optimal outcomes. Copyright © 2015 Elsevier Inc. All rights reserved.

  19. [Application of morcellator in transurethral bipolar plasmakinetic anatomical enucleation of the prostate].

    Science.gov (United States)

    Xu, A-Bai; Luo, Fu; Zou, Zhi-Hui; DU, Wei; Zhao, Peng-Peng; Liu, Chun-Xiao

    2016-08-20

    To assess the safety and therapeutic effect of morcellator in transurethral bipolar plasmakinetic anatomical enucleation (TUPKAEP) of benign prostate hyperplasia (BPH). The clinical data of 47 patients with BPH receiving TUPKAEP between January and July, 2015 were analyzed. During the operation, morcellator was used to smash the enucleated BPH which was aspirated with subatmospheric pressure in 29 cases, and the tissue was smashed with bipolar electrosurgical loop slicing from top to bottom and aspirated by ellic suction in 18 cases. s The procedures were completed successfully in all the 47 cases. The time used for adenoma dissociation was 2.24∓1.09 with morcellator at the speed of 18.43∓6.01 g/min, and was 17.19∓11.74 min with bipolar electrosurgical loop at the speed of 1.91∓0.65 g/min; the mean total operation time was significantly shorter in morcellator group (28.13∓14.71 vs 43.22∓25.39 min). The 2 groups showed no significant difference in postoperative continuous bladder irrigation time, postoperative indwelling time of urinary catheter or postoperative hospital stay. s Morcellator is safe and feasible for application in TUPKAEP and helps to shorten the operation time.

  20. A technique for evisceration as an alternative to enucleation in birds of prey: 19 cases.

    Science.gov (United States)

    Murray, Maureen; Pizzirani, Stefano; Tseng, Florina

    2013-06-01

    Ocular trauma is common in birds of prey presented to wildlife clinics and rehabilitation centers. Enucleation is the procedure most commonly described for treatment of end-stage ocular disease or chronically painful eyes in birds; however, there are several disadvantages and risks to this procedure. While evisceration has been suggested as an alternative, it has not been described for multiple cases or with long-term follow-up data in birds of prey. This report details an evisceration technique performed in 5 captive birds of prey of 4 different species (1 eastern screech owl [Megascops asio], 1 great horned owl [Bubo virginianus], 2 red-tailed hawks [Buteo jamaicensis], and 1 bald eagle [Haliaeetus leucocephalus]) with long-term follow-up information. In addition, this report describes 14 cases of free-living owls of 3 different species (1 great horned owl, 4 barred owls [Strix varia], and 9 eastern screech owls) on which this technique was performed from 2004 to 2011 and which were subsequently released to the wild. Because of the limited risk of complications and the less-severe disruption of facial symmetry, which may be particularly important in owls that are candidates for release to the wild, evisceration should be considered over enucleation in birds of prey that require surgical intervention for the management of severe sequelae to ocular trauma.

  1. Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.

    Science.gov (United States)

    Graham, David M; Andersen, Tomas; Sharek, Lisa; Uzer, Gunes; Rothenberg, Katheryn; Hoffman, Brenton D; Rubin, Janet; Balland, Martial; Bear, James E; Burridge, Keith

    2018-01-19

    The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical necessity of the nucleus during cell polarization and directed migration. Using enucleated mammalian cells (cytoplasts), we found that polarity establishment and cell migration in one dimension (1D) and two dimensions (2D) occur without the nucleus. Cytoplasts directionally migrate toward soluble (chemotaxis) and surface-bound (haptotaxis) extracellular cues and migrate collectively in scratch-wound assays. Consistent with previous studies, migration in 3D environments was dependent on the nucleus. In part, this likely reflects the decreased force exerted by cytoplasts on mechanically compliant substrates. This response is mimicked both in cells with nucleocytoskeletal defects and upon inhibition of actomyosin-based contractility. Together, our observations reveal that the nucleus is dispensable for polarization and migration in 1D and 2D but critical for proper cell mechanical responses. © 2018 Graham et al.

  2. Efficient synthesis of sulfonamide derivatives on solid supports catalyzed using solvent-free and microwave-assisted methods

    Energy Technology Data Exchange (ETDEWEB)

    Camargo-Ordonez, Argelia; Moreno-Reyes, Christian; Olazaran-Santibanez, Fabian; Martinez-Hernandez, Sheila; Bocanegra-Garcia, Virgilio; Rivera, Gildardo [Universidad Autonoma de Tamaulipas, Reynosa (Mexico). Dep. de Farmacia y Quimica Medicinal

    2011-07-01

    In this work we report the synthesis of sulfonamide derivatives using a conventional procedure and with solid supports, such as silica gel, florisil, alumina, 4A molecular sieves, montmorillonite KSF, and montmorillonite K10 using solvent-free and microwave-assisted methods. Our results show that solid supports have a catalytic activity in the formation of sulfonamide derivatives. We found that florisil, montmorillonite KSF, and K10 could be used as inexpensive alternative catalysts that are easily separated from the reaction media. Additionally, solvent-free and microwave-assisted methods were more efficient in reducing reaction time and in increasing yield. (author)

  3. Nanoparticle-assisted laser desorption/ionization mass spectrometry: Novel sample preparation methods and nanoparticle screening for plant metabolite imaging

    Energy Technology Data Exchange (ETDEWEB)

    Yagnik, Gargey B. [Iowa State Univ., Ames, IA (United States)

    2016-02-19

    The main goal of the presented research is development of nanoparticle based matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). This dissertation includes the application of previously developed data acquisition methods, development of novel sample preparation methods, application and comparison of novel nanoparticle matrices, and comparison of two nanoparticle matrix application methods for MALDI-MS and MALDI-MS imaging.

  4. A comparative study between xerographic, computer-assisted overlay generation and animated-superimposition methods in bite mark analyses.

    Science.gov (United States)

    Tai, Meng Wei; Chong, Zhen Feng; Asif, Muhammad Khan; Rahmat, Rabiah A; Nambiar, Phrabhakaran

    2016-09-01

    This study was to compare the suitability and precision of xerographic and computer-assisted methods for bite mark investigations. Eleven subjects were asked to bite on their forearm and the bite marks were photographically recorded. Alginate impressions of the subjects' dentition were taken and their casts were made using dental stone. The overlays generated by xerographic method were obtained by photocopying the subjects' casts and the incisal edge outlines were then transferred on a transparent sheet. The bite mark images were imported into Adobe Photoshop® software and printed to life-size. The bite mark analyses using xerographically generated overlays were done by comparing an overlay to the corresponding printed bite mark images manually. In computer-assisted method, the subjects' casts were scanned into Adobe Photoshop®. The bite mark analyses using computer-assisted overlay generation were done by matching an overlay and the corresponding bite mark images digitally using Adobe Photoshop®. Another comparison method was superimposing the cast images with corresponding bite mark images employing the Adobe Photoshop® CS6 and GIF-Animator©. A score with a range of 0-3 was given during analysis to each precision-determining criterion and the score was increased with better matching. The Kruskal Wallis H test showed significant difference between the three sets of data (H=18.761, pbite mark analysis using the computer-assisted animated-superimposition method was the most accurate, followed by the computer-assisted overlay generation and lastly the xerographic method. The superior precision contributed by digital method is discernible despite the human skin being a poor recording medium of bite marks. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Comparative evaluation of two reconstructive methods following laparoscopic assisted subtotal gastrectomy in dogs

    Directory of Open Access Journals (Sweden)

    Bakhtiari Jalal

    2012-12-01

    Full Text Available Abstract Background Laparoscopic gastrectomy is a new and technically challenging surgical procedure with potential benefit. The objective of this study was to investigate clinical and para-clinical consequences following Roux-en-Y and Jejunal Loop interposition reconstructive techniques for subtotal gastrectomy using laparoscopic assisted surgery. Results Following resection of the stomach attachments through a laparoscopic approach, stomach was removed and reconstruction was performed with either standard Roux-en-Y (n = 5 or Jejunal Loop interposition (n = 5 methods. Weight changes were monitored on a daily basis and blood samples were collected on Days 0, 7 and 21 post surgery. A fecal sample was collected on Day 28 after surgery to evaluate fat content. One month post surgery, positive contrast radiography was conducted at 5, 10, 20, 40, 60 and 90 minutes after oral administration of barium sulfate, to evaluate the postoperative complications. There was a gradual decline in body weight in both experimental groups after surgery (P  0.05. Fecal fat content increased in the Roux-en-Y compared to the Jejunal loop interposition technique (P  0.05. Conclusion Roux-en-Y and Jejunal loop interposition techniques might be considered as suitable approaches for reconstructing gastro-intestinal tract following gastrectomy in dogs. The results of this study warrant further investigation with a larger number of animals.

  6. Short Time and Low Temperature Reaction between Metal Oxides through Microwave-Assisted Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    S. M. V. Novais

    2016-01-01

    Full Text Available This work demonstrates the possibility of synthesis of cadmium tungstate at low temperatures using oxide precursors. Cadmium tungstate (CdWO4 scintillator was produced via microwave-assisted hydrothermal reaction using the precursors CdO and WO3. The methodology was based on microwave radiation for heating, which is remarkably faster than the solid-state route or conventional hydrothermal procedure. CdWO4 monoclinic (wolframite structure was successfully obtained at 120°C for synthesis times as short as 20 min. This route does not require the use of templates or surfactants and yields self-assembled nanorods with size of around 24 ± 9 nm width and 260 ± 47 nm length. The growth mechanism for the formation of CdWO4 involves microwave-induced dissociation of the reagents and solvation of Cd2+ and WO42- ions, which are free to move and start the nucleation process. The luminescence properties of the produced nanoparticles were investigated, presenting a broad emission band at around 500 nm, which is comparable to that observed for samples produced using other chemical routes. This result highlights the great potential of the proposed method as a low-cost and time saving process to fabricate luminescent oxide nanoparticles.

  7. A novel method for furfural recovery via gas stripping assisted vapor permeation by a polydimethylsiloxane membrane

    Science.gov (United States)

    Hu, Song; Guan, Yu; Cai, Di; Li, Shufeng; Qin, Peiyong; Karim, M. Nazmul; Tan, Tianwei

    2015-03-01

    Furfural is an important platform chemical with a wide range of applications. However, due to the low concentration of furfural in the hydrolysate, the conventional methods for furfural recovery are energy-intensive and environmentally unfriendly. Considering the disadvantages of pervaporation (PV) and distillation in furfural separation, a novel energy-efficient `green technique', gas stripping assisted vapor permeation (GSVP), was introduced in this work. In this process, the polydimethylsiloxane (PDMS) membrane was prepared by employing water as solvent. Coking in pipe and membrane fouling was virtually non-existent in this new process. In addition, GSVP was found to achieve the highest pervaporation separation index of 216200 (permeate concentration of 71.1 wt% and furfural flux of 4.09 kgm-2h-1) so far, which was approximately 2.5 times higher than that found in pervaporation at 95°C for recovering 6.0 wt% furfural from water. Moreover, the evaporation energy required for GSVP decreased by 35% to 44% relative to that of PV process. Finally, GSVP also displayed more promising potential in industrial application than PV, especially when coupled with the hydrolysis process or fermentation in biorefinery industry.

  8. Strong Antibacterial Polydopamine Coatings Prepared by a Shaking-assisted Method

    Science.gov (United States)

    Su, Lei; Yu, Yang; Zhao, Yanshuang; Liang, Feng; Zhang, Xueji

    2016-04-01

    Strong antibacterial polydopamine (PDA) coatings prepared by a facile shaking-assisted method is reported for the first time. It was found that a minor modification made to the conventional synthesis procedure of PDA coatings, viz. replacing the static solution condition with a shaking solution condition by using a mechanical shaker, can produce the roughened polydopamine (rPDA) coatings at different substrates, e.g., glass, stainless steel, plastic, and gauze. The resulting rPDA coatings were characterized with Raman spectrum, zeta-potential analysis and contact angle measurement. The antibacterial activity of the rPDA coatings was evaluated by a shake flask test with gram-positive Staphylococcus aureus, and gram-negative Escherichia coli and Pseudomonas aeruginosa as bacteria models. Testing results revealed that, in the absence of any other antibacterial agents, the rPDA coatings exhibited remarkably enhanced antibacterial activities. In addition, such enhanced antibacterial activities of the rPDA coatings were found to be unimpaired by steam sterilization treatments.

  9. Generating rate equations for complex enzyme systems by a computer-assisted systematic method

    Directory of Open Access Journals (Sweden)

    Beard Daniel A

    2009-08-01

    Full Text Available Abstract Background While the theory of enzyme kinetics is fundamental to analyzing and simulating biochemical systems, the derivation of rate equations for complex mechanisms for enzyme-catalyzed reactions is cumbersome and error prone. Therefore, a number of algorithms and related computer programs have been developed to assist in such derivations. Yet although a number of algorithms, programs, and software packages are reported in the literature, one or more significant limitation is associated with each of these tools. Furthermore, none is freely available for download and use by the community. Results We have implemented an algorithm based on the schematic method of King and Altman (KA that employs the topological theory of linear graphs for systematic generation of valid reaction patterns in a GUI-based stand-alone computer program called KAPattern. The underlying algorithm allows for the assumption steady-state, rapid equilibrium-binding, and/or irreversibility for individual steps in catalytic mechanisms. The program can automatically generate MathML and MATLAB output files that users can easily incorporate into simulation programs. Conclusion A computer program, called KAPattern, for generating rate equations for complex enzyme system is a freely available and can be accessed at http://www.biocoda.org.

  10. Gas assisted method synthesis nitrogen-doped carbon quantum dots and Hg (II) sensing.

    Science.gov (United States)

    Li, Yamei; Wang, Nan; He, Zhanhang

    2017-06-01

    Nitrogen-doped fluorescent carbon quantum dots (CQDs) was prepared by gas-assisted method using cellulose as precursors under ammonia atmosphere, which not only exhibited excellent photoluminescent properties, but also showed highly selective and sensitive detection of mercury ion. The nitrogen-doped CQDs displayed excitation wavelength dependent fluorescent behavior with outstanding dispersibility. Moreover, they exhibited high tolerance to various external conditions, such as storage time, pH value, and ionic strength. The rapid detection of Hg (II) by one-step operation within 1 min and the good linear correlation between I0/I and Hg (II) concentration in the range of 10-100 nM made the nitrogen-doped CQDs a promising nanoprobe for Hg (II) detection. The detection limit of the nitrogen-doped CQDs is about 7.7 nM. Such a nanoprobe has been successfully applied for the analysis of Hg (II) in natural water samples, demonstrating excellent practical feasibility.

  11. Ficoll-400 density gradient method as an effective sperm preparation technique for assisted reproductive techniques.

    Science.gov (United States)

    Highland, Hyacinth N; Rishika, A Sharma; Almira, S Shaikh; Kanthi, P Bansal

    2016-01-01

    Infertility being a burning issue, the male itself contributes about 40% as a cause, as evident by statistical data. However, Assisted Reproductive Technology (ART) has emerged as a powerful tool in the management of infertility. Sperm preparation techniques govern the selection procedure to separate functional spermatozoa which can then be used in IUI, IVF, and ART and for cryopreservation. The present study was aimed at evaluation of sperm preparation techniques for reliability, performance and to determine the most effective, feasible and economical technique. The subjects under study includes males with normal proven fertility (n=40) and the males with unexplained infertility (n=40). Four sperm separation techniques, viz., Swim-up, Swim-down, Sucrose and Ficoll-400 density gradient techniques were evaluated for their efficacy in separation of good quality fraction of spermatozoa. Sperm viability, morphology and maturation status of spermatozoa were taken as evaluation parameters following the standard methods (WHO 2010). Data was analyzed using student's t -test and the four selected techniques were compared with the normal semen samples for scoring the efficiency of the techniques. Out of the several techniques used, Ficoll-400 was found to be more efficient method for separation of spermatozoa. The percentage of change in each parameter was calculated and taken as the index for recovery of potent sperm from the original sperm. Ficoll-400 density gradient yielded higher percentage of live, mature, morphologically normal spermatozoa in an isolated fraction as compared to other three techniques. It was observed that a combination of Ficoll-400 gradient separation with Swim-up technique could give quality spermatozoa which in-turn would directly have an impact on the success of IVF and other ART techniques.

  12. New method to estimate the possibility of natural pregnancy using computer-assisted sperm analysis.

    Science.gov (United States)

    Isobe, Tetsuya

    2012-12-01

    The World Health Organization (WHO) criteria, which include percent motility and sperm concentration, are the only criteria for evaluating sperm quality and conception ability. However, these criteria are insufficient to evaluate the possibility of natural pregnancy. Thus, an index that can directly evaluate the possibility of a natural pregnancy is necessary. A new sperm energy theory without approximation was developed to assess the possibility of natural pregnancy based on mechanical sperm energy. Sperm motility parameters were measured using computer-assisted sperm analysis (CASA) in 129 ejaculated semen samples from 50 men in couples diagnosed with infertility, in which no abnormalities were found in women (sterile group), and 157 ejaculated semen samples from 57 men who had already fathered children in natural pregnancies (control group). A total of 129 subjects were selected from the control group and classified as the fertile group in order of the sample measurement date. The sperm energy index (SEI) and mean sperm energy index (MEI) were accurately obtained according to the methods described by the new sperm energy theory. SEI reflects total mechanical energy of the sperm in a visual field during CASA measurements. MEI reflects the mean mechanical energy of one sperm in a measurement field. All subjects with (MEI)/(SEI) > 2 were assigned to the sterile group. The larger the SEI, the higher was the probability of predicting fertile subjects. The probability of predicting fertile subjects was approximately 60% with a SEI of > 0.5, 70% with a SEI of > 1, 80% with a SEI of > 3, and 90% with a SEI of > 6 in cases where (MEI)/(SEI) is < 2. The data support the view that this novel method can be used to estimate the possibility of a natural pregnancy.

  13. The Effect of Computer Assisted and Computer Based Teaching Methods on Computer Course Success and Computer Using Attitudes of Students

    Science.gov (United States)

    Tosun, Nilgun; Sucsuz, Nursen; Yigit, Birol

    2006-01-01

    The purpose of this research was to investigate the effects of the computer-assisted and computer-based instructional methods on students achievement at computer classes and on their attitudes towards using computers. The study, which was completed in 6 weeks, were carried out with 94 sophomores studying in formal education program of Primary…

  14. Closed vessel microwave assisted extraction - An innovative method for determination of trace metals in plant materials

    Science.gov (United States)

    Oeztan, S.; Duering, R.-A.

    2012-04-01

    Determination of metal concentrations in plant samples is important for better understanding of effects of toxic metals that are biologically magnified through the food chain and compose a great danger to all living beings. In recent years the use of microwave assisted extraction for plant samples has shown tremendous research interest which will probably substitute conventional procedures in the future. Generally conventional procedures have disadvantages including consuming of time and solvents. The objective of this study is to investigate and compare a new closed vessel microwave extraction (MAE) method with the combination of EDTA (MAE-EDTA) for the determination of metal contents (Cd, Mn, Pb, Zn) in plant samples (Lolio-Cynosuretum) by ICP-OES. Validation of the method was done by comparison of the results with another MAE procedure (MAE-H) which is applied with the mixture of 69% nitric acid (HNO3) and 30% hydrogen peroxide (H2O2). Moreover, conventional plant extraction (CE) method, for which the dissolution of plant samples were handled in HNO3 after dry ashing at 420° C, was used as a reference method. Approximately 0.5 g of sample was digested in 5 ml HNO3, 3 ml H2O2, and 5 ml deionized H2O for MAE-H and in 8 ml EDTA solution for MAE-EDTA. Certified plant reference materials (CRMs) were used for comparison of recovery rates from different extraction protocols. Thereby, the applicability of both MAE-H and MAE-EDTA procedures could be demonstrated. For 58 plant samples MAE-H showed the same extraction yields as CE in the determination of trace metal contents of the investigated elements in plant samples. MAE-EDTA gave similar values when compared to MAE-H and highly linear relationships were found for determination of Cd, Mn, Pb and Zn amounts. The recoveries for the CRMs were within the range 89.6-115%. Finally, strategic characteristics of MAE-EDTA for determination metal contents (Cd, Mn, Pb, Zn) in plant samples are: (i) applicability to a large set

  15. [Transurethral enucleation of the prostate for treatment of benign prostatic hyperplasia in patients less than 50 years old].

    Science.gov (United States)

    Xu, Ya-wen; Liu, Chun-xiao; Zheng, Shao-bo; Li, Hu-ling; Fang, Ping; Chen, Bin-shen; Xu, Kai; Shen, Hai-yan

    2010-12-01

    To evaluate the therapeutic effect of transurethral enucleation of the prostate for treatment of benign prostatic hyperplasia in patients below 50 years of age. Twelve patients with benign prostatic hyperplasia patients (mean age 48.2 years, range 46-49 years) underwent transurethral enucleation of the prostate. The middle lobe and two lateral lobes were enucleated with the preprosthetic sphincter and anterior fibromuscular stroma preserved during the operation. The patients were followed up to evaluate the lower urinary tract symptoms and sexual activity after the surgery. The 12 patients were followed up for 3 to 6 months. The symptoms of lower urinary tract obstruction were improved obviously after the surgery, and the International Prostate Symptom Score (IPSS) decreased from 24±5.1 to 8.8±1.4 and peak urine flow rate (Qmax) increased from 8.1±4.2 ml/s to 20.1±4.2 ml/s at 3 months postoperatively. All the 12 cases had residual urine (12-44 ml) preoperatively, but after the surgery, only 4 still had residual urine of less than 30 ml. All the patients had normal erection function postoperatively, and 10 had normal ejaculation; the other 2 patients recovered normal ejaculation 3 and 5 months after the operation, respectively. Transurethral enucleation can alleviate the low urinary tract obstruction symptom and improve the sexual function by avoiding preprosthetic sphincter injury in relatively young patients with benign prostatic hyperplasia.

  16. Predictive risk factors of postoperative urinary incontinence following holmium laser enucleation of the prostate during the initial learning period.

    Science.gov (United States)

    Kobayashi, Shuichiro; Yano, Masataka; Nakayama, Takayuki; Kitahara, Satoshi

    2016-01-01

    To determine the predictive factors for postoperative urinary incontinence (UI) following holmium laser enucleation of the prostate (HoLEP) during the initial learning period. We evaluated 127 patients with benign prostatic hyperplasia who underwent HoLEP between January 2011 and December 2013. We recorded clinical variables, including blood loss, serum prostate-specific antigen levels, and the presence or absence of UI. Blood loss was estimated as a decline in postoperative hemoglobina levels. The predictive factors for postoperative UI were determined using a multivariable logistic regression analysis. Postoperative UI occurred in 31 patients (24.4%), but it cured in 29 patients (93.5%) after a mean duration of 12 weeks. Enucleation time >100 min (p=0.043) and blood loss >2.5g/dL (p=0.032) were identified as significant and independent risk factors for postoperative UI. Longer enucleation time and increased blood loss were independent predictors of postoperative UI in patients who underwent HoLEP during the initial learning period. Surgeons in training should take care to perform speedy enucleation maneuver with hemostasis. Copyright© by the International Brazilian Journal of Urology.

  17. Comparisons between conventional, ultrasound-assisted and microwave-assisted methods for extraction of anthraquinones from Heterophyllaea pustulata Hook f. (Rubiaceae).

    Science.gov (United States)

    Barrera Vázquez, M F; Comini, L R; Martini, R E; Núñez Montoya, S C; Bottini, S; Cabrera, J L

    2014-03-01

    This work reports a comparative study about extraction methods used to obtain anthraquinones (AQs) from stems and leaves of Heterophyllae pustulata Hook (Rubiáceae). One of the conventional procedures used to extract these metabolites from a vegetable matrix is by successive Soxhlet extractions with solvents of increasing polarity: starting with hexane to eliminate chlorophylls and fatty components, following by benzene and finally ethyl acetate. However, this technique shows a low extraction yield of total AQs, and consumes large quantities of solvent and time. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have been investigated as alternative methods to extract these compounds, using the same sequence of solvents. It was found that UAE increases the extraction yield of total AQs and reduces the time and amount of solvent used. Nevertheless, the combination UAE with benzene, plus MAE with ethyl acetate at a constant power of 900 W showed the best results. A higher yield of total AQs was obtained in less time and using the same amount of solvent that UAE. The optimal conditions for this latter procedure were UAE with benzene at 50 °C during 60 min, followed by MAE at 900 W during 15 min using ethyl acetate as extraction solvent. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. Novel Method for Exchange of Impella Circulatory Assist Catheter: The "Trojan Horse" Technique.

    Science.gov (United States)

    Phillips, Colin T; Tamez, Hector; Tu, Thomas M; Yeh, Robert W; Pinto, Duane S

    2017-07-01

    Patients with an indwelling Impella may require escalation of hemodynamic support or exchange to another circulatory assistance platform. As such, preservation of vascular access is preferable in cases where anticoagulation cannot be discontinued or to facilitate exchange to an alternative catheter or closure device. Challenges exist in avoiding bleeding and loss of wire access in these situations. We describe a single-access "Trojan Horse" technique that minimizes bleeding while maintaining arterial access for rapid exchange of this percutaneous ventricular assist device.

  19. Analysis of Contemporary Methods for Designing Rotary Type Ventricular Assist Devices

    Directory of Open Access Journals (Sweden)

    E. P. Banin

    2015-01-01

    Full Text Available The research object is inlet apparatus of ventricular assist device, namely inlet cannula and straightener.The purpose of the study is to reveal features of blood flow in inlet apparatus of ventricular assist device. The mathematical modeling is carried out by computational fluid dynamics analysis in a stationary setting.The first part of study concerns the analysis of existing approaches to the numerical and experimental studies in designing the ventricular assist devices of rotary type. It reveals the features of each approach for their further application in practice. The article presents an original design of developed hydraulic test bench to verify the results of mathematical modeling. Analysis of foreign authors’ studies showed that there is no enough attention paid to design of the adjacent pump assemblies of ventricular assist device. The second part of study considers direct mathematical modeling of input apparatus of ventricular assist device. The study examined straightener with three or four blades. Mathematical modeling has revealed the presence of potentially dangerous stagnation zones and essential asymmetry of the outlet flow from the input unit. The found features must be taken in consideration in designing the ventricular assist device pumps. In the future we plan to use obtained data to create a parametric model of the rotor and the diffuser considering the abovementioned features.

  20. Ultrasound-Assist Extrusion Methods for the Fabrication of Polymer Nanocomposites Based on Polypropylene/Multi-Wall Carbon Nanotubes

    Science.gov (United States)

    Ávila-Orta, Carlos A.; Quiñones-Jurado, Zoe V.; Waldo-Mendoza, Miguel A.; Rivera-Paz, Erika A.; Cruz-Delgado, Víctor J.; Mata-Padilla, José M.; González-Morones, Pablo; Ziolo, Ronald F.

    2015-01-01

    Isotactic polypropylenes (iPP) with different melt flow indexes (MFI) were used to fabricate nanocomposites (NCs) with 10 wt % loadings of multi-wall carbon nanotubes (MWCNTs) using ultrasound-assisted extrusion methods to determine their effect on the morphology, melt flow, and electrical properties of the NCs. Three different types of iPPs were used with MFIs of 2.5, 34 and 1200 g/10 min. Four different NC fabrication methods based on melt extrusion were used. In the first method melt extrusion fabrication without ultrasound assistance was used. In the second and third methods, an ultrasound probe attached to a hot chamber located at the exit of the die was used to subject the sample to fixed frequency and variable frequency, respectively. The fourth method is similar to the first method, with the difference being that the carbon nanotubes were treated in a fluidized air-bed with an ultrasound probe before being used in the fabrication of the NCs with no ultrasound assistance during extrusion. The samples were characterized by MFI, Optical microscopy (OM), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), electrical surface resistivity, and electric charge. MFI decreases in all cases with addition of MWCNTs with the largest decrease observed for samples with the highest MFI. The surface resistivity, which ranged from 1013 to 105 Ω/sq, and electric charge, were observed to depend on the ultrasound-assisted fabrication method as well as on the melt flow index of the iPP. A relationship between agglomerate size and area ratio with electric charge was found. Several trends in the overall data were identified and are discussed in terms of MFI and the different fabrication methods. PMID:28793686

  1. Ultrasound-Assist Extrusion Methods for the Fabrication of Polymer Nanocomposites Based on Polypropylene/Multi-Wall Carbon Nanotubes

    Directory of Open Access Journals (Sweden)

    Carlos A. Ávila-Orta

    2015-11-01

    Full Text Available Isotactic polypropylenes (iPP with different melt flow indexes (MFI were used to fabricate nanocomposites (NCs with 10 wt % loadings of multi-wall carbon nanotubes (MWCNTs using ultrasound-assisted extrusion methods to determine their effect on the morphology, melt flow, and electrical properties of the NCs. Three different types of iPPs were used with MFIs of 2.5, 34 and 1200 g/10 min. Four different NC fabrication methods based on melt extrusion were used. In the first method melt extrusion fabrication without ultrasound assistance was used. In the second and third methods, an ultrasound probe attached to a hot chamber located at the exit of the die was used to subject the sample to fixed frequency and variable frequency, respectively. The fourth method is similar to the first method, with the difference being that the carbon nanotubes were treated in a fluidized air-bed with an ultrasound probe before being used in the fabrication of the NCs with no ultrasound assistance during extrusion. The samples were characterized by MFI, Optical microscopy (OM, Scanning electron microscopy (SEM, Transmission electron microscopy (TEM, electrical surface resistivity, and electric charge. MFI decreases in all cases with addition of MWCNTs with the largest decrease observed for samples with the highest MFI. The surface resistivity, which ranged from 1013 to 105 Ω/sq, and electric charge, were observed to depend on the ultrasound-assisted fabrication method as well as on the melt flow index of the iPP. A relationship between agglomerate size and area ratio with electric charge was found. Several trends in the overall data were identified and are discussed in terms of MFI and the different fabrication methods.

  2. Evaluation of vacuum-assisted biopsy as a new method for histological diagnosis of soft-tissue tumors

    OpenAIRE

    Klein, Torsten

    2010-01-01

    Background: The exact pre-operative diagnosis of a low invasive and complication-poor biopsy-method is often problematic. Often, the taken tissue-specimen is not sufficient impeded in order to investigate the exact histopathology of soft-tissue-tumors, which complicates an adequate operative therapy. Vacuum-assisted-biopsy is a new method whose valence histopathology diagnosis for the praeoperative of soft-tissue-tumors, has not yet examined scientifically. The feasibility and the exact pre-o...

  3. Diffusion tensor imaging detects early cerebral cortex abnormalities in neuronal architecture induced by bilateral neonatal enucleation: An experimental model in the ferret

    Directory of Open Access Journals (Sweden)

    Andrew S Bock

    2010-10-01

    Full Text Available Diffusion tensor imaging (DTI is a technique that non-invasively provides quantitative measures of water translational diffusion, including fractional anisotropy (FA, that are sensitive to the shape and orientation of cellular elements, such as axons, dendrites and cell somas. For several neurodevelopmental disorders, histopathological investigations have identified abnormalities in the architecture of pyramidal neurons at early stages of cerebral cortex development. To assess the potential capability of DTI to detect neuromorphological abnormalities within the developing cerebral cortex, we compare changes in cortical FA with changes in neuronal architecture and connectivity induced by bilateral enucleation at postnatal day 7 (BEP7 in ferrets. We show here that the visual callosal pattern in BEP7 ferrets is more irregular and occupies a significantly greater cortical area compared to controls at adulthood. To determine whether development of the cerebral cortex is altered in BEP7 ferrets in a manner detectable by DTI, cortical FA was compared in control and BEP7 animals on postnatal day 31. Visual cortex, but not rostrally-adjacent non-visual cortex, exhibits higher FA than control animals, consistent with BEP7 animals possessing axonal and dendritic arbors of reduced complexity than age-matched controls. Subsequent to DTI, Golgi staining and analysis methods were used to identify regions, restricted to visual areas, in which the orientation distribution of neuronal processes is significantly more concentrated than in control ferrets. Together, these findings suggest that DTI can be of utility for detecting abnormalities associated with neurodevelopmental disorders at early stages of cerebral cortical development, and that the neonatally-enucleated ferret is a useful animal model system for systematically assessing the potential of this new diagnostic strategy.

  4. The safety and efficacy of front-firing green-light laser endoscopic en bloc photoselective vapo-enucleation of non-muscle-invasive bladder cancer

    Directory of Open Access Journals (Sweden)

    Cheng B

    2017-08-01

    Full Text Available Bo Cheng,1,2 Xiaofu Qiu,1 Huanhui Li,1 Guosheng Yang1 1Department of Urology, Southern Medical University affiliated Guangdong Second Provincial General Hospital, Southern Medical University, Guangzhou, People’s Republic of China; 2Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China Purpose: Laser therapy provides an alternative option for treating non-muscle-invasive bladder cancer (NMIBC. However, the clinical evidence for potassium-titanyl-phosphate (KTP laser en bloc resection is still limited. Here, we investigated the efficacy and safety of the 120-W front-firing KTP laser for the treatment of NMIBC.Methods: A total of 64 patients with NMIBC treated with either a 120-W front-firing KTP-photoselective vapo-enucleation of the bladder tumor (PVEBT, n=34 or transurethral resection of the bladder tumor (TURBT, n=30 were included. En bloc resection was applied to the patients in PVEBT group.Results: There was no significant difference in rinsing time (P=0.292, indwelling catheter (P=0.080, pathologic type, and T stage (P=0.870 between the two groups. Compared with the TURBT group, patients treated with PVEBT had a shorter hospitalization stay (P=0.044, a shorter operation time (P=0.008, and a lower muscle miss rate (P=0.044. PVEBT is superior to TURBT in terms of the rate of 1-year recurrence (P=0.015 and tumor grade progression rate (P=0.019.Conclusion: The 120-W front-firing KTP laser en bloc enucleation technique is a safe and feasible procedure for treating patients with NMIBC. Further external validation in larger cohorts with a long follow-up period is warranted. Keywords: bladder cancer, transurethral resection, en bloc, laser surgery, recurrence

  5. Shape effect on the antibacterial activity of silver nanoparticles synthesized via a microwave-assisted method.

    Science.gov (United States)

    Hong, Xuesen; Wen, Junjie; Xiong, Xuhua; Hu, Yongyou

    2016-03-01

    Silver nanoparticles (AgNPs) are used as sustained-release bactericidal agents for water treatment. Among the physicochemical characteristics of AgNPs, shape is an important parameter relevant to the antibacterial activity. Three typically shaped AgNPs, nanocubes, nanospheres, and nanowires, were prepared via a microwave-assisted method and characterized by TEM, UV-vis, and XRD. The antibacterial activity of AgNPs was determined by OD growth curves tests, MIC tests, and cell viability assay against Escherichia coli. The interaction between AgNPs and bacterial cells was observed by TEM. The results showed that the three differently shaped AgNPs were nanoscale, 55 ± 10 nm in edge length for nanocubes, 60 ± 15 nm in diameter for nanospheres, 60 ± 10 nm in diameter and 2-4 μm in length for nanowires. At the bacterial concentration of 10(4) CFU/mL, the MIC of nanocubes, nanospheres, and nanowires were 37.5, 75, and 100 μg/mL, respectively. Due to the worst contact with bacteria, silver nanowires exhibited the weakest antibacterial activity compared with silver nanocubes and silver nanospheres. Besides, silver nanocubes mainly covered by {100} facets showed stronger antibacterial activity than silver nanospheres covered by {111} facets. It suggests that the shape effect on the antibacterial activity of AgNPs is attributed to the specific surface areas and facets reactivity; AgNPs with larger effective contact areas and higher reactive facets exhibit stronger antibacterial activity.

  6. Simple method to predict return of continence after robot-assisted radical prostatectomy.

    Science.gov (United States)

    Skarecky, Douglas; Morales, Blanca; Chang, Alexandra; Ahlering, Thomas

    2011-09-01

    After removal of the Foley catheter after robot-assisted radical prostatectomy (RARP), recovery of continence can take days to months. We sought to identify a simple means to predict time to recovery of postoperative continence. Preoperative characteristics on 172 men who were undergoing RARP were entered into an electronic database. All men were queried via telephone and/or returned a 7-day log of pad use. Men without need for pads were excluded (n=41). At 4 to 7 days, responses were grouped as: one pad (n=55), two pads (n=35), or three or more pads (n=41). Patients returned self-addressed postcards noting the date of 0-pad urinary status. Univariate and multivariate analysis of variables were assessed for ability to predict time to continence. No preoperative factors, such as age, International Index of Erectile Function-5, prostate-specific antigen level, American Urological Association symptom score, body mass index, uroflowmetry, nerve-sparing status, estimated blood loss, or prostate weight, were found to predict time to continence. Pad use at 4 to 7 days, however, was highly correlated with median time to continence. The median time to continence for men using one pad was 35 days, two pads was 42 days, and for three or more pads was 73 days (P=0.0001). As has been previously reported, we found no reliable baseline factors that predicted postoperative time to 0-pad continence. We did find that determining pad usage at 4 to 7 days after catheter removal strongly predicted time to pad-free continence. This method is simpler then pad weights, predicts high- and low-risk men for delayed continence, and can be used for counseling/intervention.

  7. Late effects of orbital enucleation and radiation on maxillofacial prosthetic rehabilitation: A clinical report.

    Science.gov (United States)

    Tummawanit, Supanut; Shrestha, Binit; Thaworanunta, Sita; Srithavaj, Theerathavaj

    2013-05-01

    The retinoblastoma is one of the most common tumors of the eye diagnosed in childhood. The treatment for patients with retinoblastoma includes surgical removal of the lesion along with adjunctive chemotherapy and radiotherapy. If the tumor is controlled, these treatments can have secondary adverse effects pertaining to the growth and development of orofacial structures in young patients. This clinical report describes the prosthetic rehabilitation of a patient who underwent enucleation followed by radiation therapy and adjunctive chemotherapy to treat the primary diagnosis of retinoblastoma. This therapy resulted in a combination of dental and facial growth and developmental abnormalities. Copyright © 2013 The Editorial Council of the Journal of Prosthetic Dentistry. Published by Mosby, Inc. All rights reserved.

  8. [Initial results of transurethral enucleation with bipolar system for benign prostate hypertrophy patients].

    Science.gov (United States)

    Nakao, Atsushi; Fukui, Koji; Togo, Yoshikazu; Kokura, Koji

    2010-07-01

    We have performed transurethral enucleation with bipolar system (TUEB) on 60 patients since April 2008. The patients were 61 to 81 years old (average 71.7 years old), and estimated prostate volumes were 25 cm3 to 80.43 cm3 (average 51.1 cm3). The weight of prostate removed was 8 g to 56 g (average 27.4 g) during the operations which lasted between 40 min to 200 min (average 117.5 min). The International Prostate Symptom Score (IPSS), quality of life index (QOL) maximum flow rate (Q max) and average flow rate (Qave) were recorded before operation, and at 1 and at 3 months after operation. The results indicated a high safety with TUEB compared to TUR-P even for beginners. In conclusion, TUEB may become the most common approach in the treatment of BPH.

  9. Holmium Laser Enucleation of the Prostate (HoLEP in Ambulatory Surger

    Directory of Open Access Journals (Sweden)

    Marc Fourmarier

    2015-05-01

    Full Text Available The focus of this work is the need to give an update on the holmium laser enucleation of the prostate (HoLEP technique in ambulatory surgery. Indeed, over the last two decades, there has been a significant change in surgical treatment of benign prostatic hyperplasia. Laser surgery has been growing in popularity as an alternative to standard transurethral prostatectomy. Our goal was to analyse the opportunity to perform HoLEP in one-day surgery. Furthermore, there is a willingness of the French Ministry of Health to develop this kind of management. A pilot study was performed in 50 selected patients to evaluate HoLEP feasibility in ambulatory surgery from June 2013 to April 2014. The results were good with minimal morbidity and a high satisfaction rate, but excellent organisation is necessary, leaving no room for improvisation.

  10. Influence of prostate size on the outcome of holmium laser enucleation of the prostate.

    Science.gov (United States)

    Shah, Hemendra N; Sodha, Hiren S; Kharodawala, Shabbir J; Khandkar, Amit A; Hegde, Sunil S; Bansal, Manish B

    2008-06-01

    To analyse the effect of prostate size on the outcome of holmium laser enucleation of prostate (HoLEP, an established procedure for treating symptomatic benign prostatic hypertrophy, BPH), in the initial 354 patients at 1 year of follow-up. We retrospectively reviewed the records of 354 patients who had HoLEP at our institution from April 2003 to March 2007. In 235 patients the prostate weighed 100 g (group 3). Demographic data and perioperative variables were recorded and compared among the three groups. The mean prostate size was 38.1, 76.4 and 133.5 g for groups 1, 2 and 3, respectively (P prostate was 18.47, 40.8 and 82.76 g, respectively (P prostate size, and is associated with low morbidity. The efficiency of HoLEP increases with increasing prostate size.

  11. Prosthetic rehabilitation of surgically treated orbital defects - evisceration, enucleation, and exenteration: A case series

    Directory of Open Access Journals (Sweden)

    Anna Serene Babu

    2016-01-01

    Full Text Available The rehabilitation of a patient who has suffered the psychological trauma due to loss of an eye requires a prosthesis that will provide the optimum cosmetic and functional result. The mode of rehabilitation varies based on the type of defect and surgical approach being adopted. A case series of prosthetic rehabilitation of three types of orbital defects - evisceration, enucleation and exenteration have been reported in this article. The clinical relevance of surgical approaches highlights the preservation of remaining anatomic structures creating a negative space or concavity to aid in future prosthetic rehabilitation. A multidisciplinary management and team approach is essential in providing esthetics and to regain the confidence. Follow-up care for the patient is mandatory.

  12. One-year Surgical Outcomes of Complete or Incomplete Enucleation of Prostate by Monopolar Electrocoagulation, Photoselective Vapoenucleation of 120-W GreenLight Laser, and Holmium Laser.

    Science.gov (United States)

    Cho, Sung Yong; Park, Juhyun; Yoo, Sangjun; Cho, Min Chul; Jeong, Hyeon; Son, Hwancheol

    2017-10-01

    To evaluate surgical outcomes of patients who underwent complete or incomplete enucleation technique during a short-term postoperative period. Patients having intractable lower urinary tract symptoms/benign prostatic hyperplasia and prostates >30 g with obstructed pattern in the urodynamic examinations were included. They underwent transurethral resection of prostate (TUR-P), 120 W GreenLight laser high power system-photoselective vapoenucleation of prostate (HPS-PVEP), or holmium laser enucleation of prostate (HoLEP). Patients with the size of remnant prostates minus surgical defects enucleated group (group C), and others were grouped into the partially enucleated group (group P). Mean prostate-specific antigen value was 3.5 ± 4.5 ng/mL, and mean prostate volume was 58.4 ± 31.0 mL. Complete enucleation rates in TUR-P, HPS-PVEP, and HoLEP groups were 39% (37 out of 95), 54.6% (83 out of 152), and 54.4% (31 out of 57), respectively. Complete enucleation rate of the TUR-P was significantly lower than those of the other 2 groups. Compared with group C, group P had lower maximal flow rate, higher bladder outlet obstruction index, and higher overactive bladder symptom scores. Multivariate logistic regression analyses showed that smaller prostate, presence of intravesical prostatic protrusion, HoLEP operation, and surgeons' experience were significant predictors for achieving complete enucleation of prostate. Voiding subscores of group C were significantly higher than those of group P at postoperative 12 months. The performance of HoLEP was superior to other surgical techniques. However, HPS-PVEP with vapoenucleation showed the comparable enucleation rate with that of HoLEP. Complete enucleation was effective in maintaining outcomes of prostate resection, especially voiding subscores. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Cortical potentials after electrical intraneural stimulation of the optic nerve during orbital enucleation.

    Science.gov (United States)

    Benedičič, Mitja; Beltram, Matej; Olup, Brigita Drnovšek; Bošnjak, Roman

    2012-12-01

    The aim of this study was to present cortical potentials after electrical intraneural stimulation of the optic nerve during orbital enucleation due to malignant melanoma of the choroid or the ciliary body. These cortical potentials were related to cortical potentials after electrical epidural stimulation of the optic nerve, recorded during non-manipulative phases of neurosurgery for central skull base tumors. Cortical potentials were recorded with surface occipital electrode (Oz) in six patients undergoing orbital enucleation under total intravenous anesthesia. Two thin needle stimulating electrodes were inserted inside the intraorbital part of the optic nerve. The electrical stimulus consisted of a rectangular current pulse of varying intensity (0.2-10.0 mA) and duration (0.1-0.3 ms); the stimulation rate was 2 Hz; the bandpass filter was 1-1,000 Hz; the analysis time was 50-300 ms. Cortical potentials could not be obtained or were inconsistently elicitable in three patients with longstanding history (>3 months) of severe visual deterioration, while they consisted of several positive and negative deflections in a patient with a short history of mild visual impairment. In two other patients, cortical potentials consisted of N20, P30 and N40 waves. Cortical potentials after electrical intraneural stimulation of the optic nerve could be recorded in patients with a short history of visual deterioration and without optic nerve atrophy and appear more heterogeneous than cortical potentials after electrical epidural stimulation of the optic nerve, recorded during non-manipulative phases of neurosurgery for central skull base tumors.

  14. Observations and recommendations for further research regarding environmentally assisted fatigue evaluation methods

    Energy Technology Data Exchange (ETDEWEB)

    Stevens, G.L.; Tregoning, R.L. [U.S. Nuclear Regulatory Commission (U.S. NRC), Rockville, MD (United States); Chopra, O.K. [Argonne National Laboratory, Argonne, IL (United States)

    2014-07-01

    The U.S. Nuclear Regulatory Commission (NRC) and Argonne National Laboratory (ANL) have completed research activities on environmentally assisted fatigue (EAF) methods. This work has led to a revision of NUREG/CR-6909 in its entirety, a draft of which is forthcoming for public review and comment. These activities addressed the following areas: - Air and water fatigue curves were re-developed using a much larger fatigue (ε-N) database. The additional data include an expansion in the ε-N data previously used by the NRC and ANL by as much as 50%. - The environmental fatigue multiplier (F{sub en}) expressions for carbon, low-alloy, stainless, and nickel-alloy steels were revised. - The revised F{sub en} expressions address comments from interested stakeholders related to: (a) the constants in previous F{sub en} expressions that results in F{sub en} values of approximately 2.0 even when the strain rate is very high or the temperature is very low, (b) the temperature dependence of the F{sub en} expression for carbon and low-alloy steels, and (c) the dependence of F{sub en} on water chemistry for austenitic stainless steels. - In addition, the appropriateness of a strain threshold and the possible effects of hold periods were evaluated. - The potential effects of dynamic strain aging (DSA) on cyclic deformation and environmental effects were discussed. - The revised F{sub en} expressions proposed were validated to the extent possible by comparing the results of five different experimental data sets obtained from fatigue tests that simulate actual plant conditions to estimates of fatigue usage adjusted for environmental effects using the updated F{sub en} expressions. In the course of performing the foregoing EAF research activities, the NRC and ANL observed several areas where further research could yield reduced conservatism in EAF evaluation. These include more refined, material-specific fatigue (S-N) curves, S-N curves for ferritic materials based on material tensile

  15. Holmium laser enucleation of the prostate: surgical, functional, and quality-of-life outcomes upon extended follow-up

    Directory of Open Access Journals (Sweden)

    Ilter Alkan

    2016-04-01

    Full Text Available ABSTRACT Objectives: To evaluate the long-term surgical, functional, and quality-of-life (QoL outcomes after Holmium laser enucleation of the prostate (HoLEP in patients with symptomatic benign prostatic hyperplasia (BPH. Materials and Methods: We retrospectively reviewed recorded data on patients who underwent HoLEP between June 2002 and February 2005. Ninety-six patients were enrolled. Demographic, perioperative, and postoperative data were recorded. On follow-up, International Prostate Symptom Scores (IPSSs, prostate-specific antigen (PSA levels, QoL scores, peak uroflowmetric data (Qmax values, and post-voiding residual urine volumes (PVR volumes, were recorded. Complications were scored using the Clavien system. Statistical significance was set at p<0.05. Results: The mean follow-up time was 41.8±34.6 months and the mean patient age 73.2±8.7 years. The mean prostate volume was 74.6±34.3mL. Significant improvements in Qmax values, QoL, and IPSSs and decreases in PSA levels and PVR volumes were noted during follow-up (all p values=0.001. The most common complication was a requirement for re-catheterisation because of urinary retention. Two patients had concomitant bladder tumours that did not invade the muscles. Eight patients (8.3% required re-operations; three had residual adenoma, three urethral strictures, and two residual prostate tissue in the bladder. Stress incontinence occurred in one patient (1%. All complications were of Clavien Grade 3a. We noted no Clavien 3b, 4, or 5 complications during follow-up. Conclusions: HoLEP improved IPSSs, Qmax values, PVR volumes, and QoL and was associated with a low complication rate, during extended follow-up. Thus, HoLEP can be a viable option to transurethral resection of the prostate.

  16. Predictors of urgency improvement after Holmium laser enucleation of the prostate in men with benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Won Sok Hur

    2016-11-01

    Full Text Available Purpose: To investigate the change in urinary urgency and predictors of urgency improvement after holmium laser enucleation of the prostate (HoLEP in men with benign prostatic hyperplasia (BPH. Materials and Methods: We retrospectively analyzed the medical records of patients who were treated with HoLEP for BPH and had preoperative urgency measuring ≥3 on a 5-point urinary sensation scale. Those with prostate cancer diagnosed prior to or after HoLEP, a history of other prostatic and/or urethral surgery, moderate to severe postoperative complications, and neurogenic causes were excluded. Patients who had improved urgency with antimuscarinic medication after HoLEP were excluded. We divided the patients into 2 groups based on urgency symptoms 3 months after HoLEP: improved and unimproved urgency. Improved urgency was defined as a reduction of 2 or more points on the 5-point urinary sensation scale. Preoperative clinical and urodynamic factors as well as perioperative factors were compared between groups. Results: In total, 139 patients were included in this study. Voiding parameters in all patients improved significantly after HoLEP. Seventy-one patients (51.1% had improved urgency, while 68 (48.9% did not show any improvement. A history of acute urinary retention (AUR and postvoid residual were associated with postoperative urgency improvement in univariate analysis. In multivariate analysis, a history of AUR was an independent factor affecting urgency improvement. Conclusions: A preoperative history of AUR could influence the change in urgency after HoLEP surgery in patients with BPH.

  17. A Dynamic Localized Adjustable Force Field Method for Real-Time Assistive Non-Holonomic Mobile Robotics

    Directory of Open Access Journals (Sweden)

    Michael Gillham

    2015-10-01

    Full Text Available Providing an assistive navigation system that augments rather than usurps user control of a powered wheelchair represents a significant technical challenge. This paper evaluates an assistive collision avoidance method for a powered wheelchair that allows the user to navigate safely whilst maintaining their overall governance of the platform motion. The paper shows that by shaping, switching and adjusting localized potential fields we are able to negotiate different obstacles by generating a more intuitively natural trajectory, one that does not deviate significantly from the operator in the loop desired-trajectory. It can also be seen that this method does not suffer from the local minima problem, or narrow corridor and proximity oscillation, which are common problems that occur when using potential fields. Furthermore this localized method enables the robotic platform to pass very close to obstacles, such as when negotiating a narrow passage or doorway.

  18. Transurethral surgical anatomy of the arterial bleeder in the enucleated capsular plane of enlarged prostates during holmium laser enucleation of the prostate.

    Science.gov (United States)

    Choo, Min Soo; Lee, Hahn-Ey; Bae, Jungbum; Cho, Sung Yong; Oh, Seung-June

    2014-09-01

    To identify the endoscopic vascular anatomy of the prostate during Holmium laser enucleation of the prostate (HoLEP), and analyze the clinical risk factors associated with significant arterial bleeding. We identified 107 consecutive patients with benign prostatic hyperplasia who underwent HoLEP between September 2009 and August 2010, performed by a single surgeon (S.J.O.). Two independent reviewers reviewed the surgery video database and completed a prespecified form. The location of bleeding arteries was marked at the level of the bladder neck, proximal prostate, distal prostate, and verumontanum. Arterial bleeding was classified into one of three grades according to bleeding severity (grades 2 and 3 indicate significant bleeding). The mean prostate volume was 65.1±31.5 mL, and the mean prostate-specific antigen (PSA) level was 3.69±3.58 ng/mL. During the HoLEP procedure, the most common locations of significant bleeders were the 2-5 and 7-10 o'clock positions in the proximal prostate. The average number of bleeding arteries was 12.1±7.9 per procedure, and 1.93±1.20 per 10 mL of prostate volume. Multivariate analysis revealed that prostate volume and serum PSA were significant parameters for estimating the number of bleeding vessels. During the HoLEP procedure, the most common locations of significant bleeders were the 2-5 and 7-10 o'clock positions in the proximal prostate. Prostate volume was associated with the number of bleeders. A careful approach to the capsular plane of the proximal prostate facilitates early hemostasis during the HoLEP procedure, especially with larger adenomas.

  19. [Computer-assisted teaching in medical education--development of methods and supplemental materials].

    Science.gov (United States)

    Santek, F; Bozikov, J; Kern, J; Dezelić, G

    1990-01-01

    A review of the elementary types of materials for the computer assisted learning (CAI--Computer Assisted Instruction) is given in this paper. It shows our initial experiences in creating the computer educational materials of the patient management simulation type, destinated for the primary care physicians. A computer simulation program "First Aid in Traffic Accident" is described as an example. A typical structure of these materials is shown. The materials had been developed using the own authoring system, a computer program which enables the teachers and other experts to create the computer educational materials independently, with the minimal help of the professional computer experts.

  20. Characterization methods of nano-patterned surfaces generated by induction heating assisted injection molding

    DEFF Research Database (Denmark)

    Tang, Peter Torben; Ravn, Christian; Menotti, Stefano

    2015-01-01

    An induction heating-assisted injection molding (IHAIM) process developed by the authors is used to replicate surfaces containing random nano-patterns. The injection molding setup is developed so that an induction heating system rapidly heats the cavity wall at rates of up to 10◦C/s. In order...

  1. Portfolio : Theory and Practice of the Pedagogical Methods Course for Assistant Professors at Aalborg University

    DEFF Research Database (Denmark)

    Hansen, Søren

    2002-01-01

    At Aalborg University staff development is organized as a pedagogical course that all Assistant Professors must attend. The pedagogical principles are that learning takes place in action and by keeping a reflective dialog up with supervisors, students and colleagues. Relevant theory is learned from...

  2. New method for sternal closure after vacuum-assisted therapy in deep sternal infections after cardiac surgery.

    Science.gov (United States)

    Reiss, Nils; Schuett, Ulrich; Kemper, Michael; Bairaktaris, Andreas; Koerfer, Reiner

    2007-06-01

    The treatment of nonhealing and infected sternotomies after cardiac surgery is a challenging task with increased rates of mortality and morbidity, as well as high costs. A local vacuum therapy (ie, the vacuum-assisted closure system) permits the treatment of deep sternal infections due to continuous aspiration and a sealed dressing that stimulates granulation tissue formation. Aggressive vacuum-assisted closure treatment of the sternum in postoperative deep wound infection enhances sternal preservation and the speed of potential rewiring. After some weeks of vacuum-assisted closure therapy, a complete preparation of the substernal structures is necessary. In this context, laceration of the right ventricle is a rare but life-threatening complication. We describe a new technique for sternal closure after vacuum-assisted wound treatment using Nitinol clips (Praesidia, Bologna, Italy), which can prevent these severe complications. Without any preparation of the substernal tissue the clips can be inserted in the parasternal space with consecutive proper stabilization of the sternum. This new method represents an easy, low-cost and complication-free procedure.

  3. Improvement of a sample preparation method assisted by sodium deoxycholate for mass-spectrometry-based shotgun membrane proteomics.

    Science.gov (United States)

    Lin, Yong; Lin, Haiyan; Liu, Zhonghua; Wang, Kunbo; Yan, Yujun

    2014-11-01

    In current shotgun-proteomics-based biological discovery, the identification of membrane proteins is a challenge. This is especially true for integral membrane proteins due to their highly hydrophobic nature and low abundance. Thus, much effort has been directed at sample preparation strategies such as use of detergents, chaotropes, and organic solvents. We previously described a sample preparation method for shotgun membrane proteomics, the sodium deoxycholate assisted method, which cleverly circumvents many of the challenges associated with traditional sample preparation methods. However, the method is associated with significant sample loss due to the slightly weaker extraction/solubilization ability of sodium deoxycholate when it is used at relatively low concentrations such as 1%. Hence, we present an enhanced sodium deoxycholate sample preparation strategy that first uses a high concentration of sodium deoxycholate (5%) to lyse membranes and extract/solubilize hydrophobic membrane proteins, and then dilutes the detergent to 1% for a more efficient digestion. We then applied the improved method to shotgun analysis of proteins from rat liver membrane enriched fraction. Compared with other representative sample preparation strategies including our previous sodium deoxycholate assisted method, the enhanced sodium deoxycholate method exhibited superior sensitivity, coverage, and reliability for the identification of membrane proteins particularly those with high hydrophobicity and/or multiple transmembrane domains. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Gravity-Assist Trajectories to the Ice Giants: An Automated Method to Catalog Mass-or Time-Optimal Solutions

    Science.gov (United States)

    Hughes, Kyle M.; Knittel, Jeremy M.; Englander, Jacob A.

    2017-01-01

    This work presents an automated method of calculating mass (or time) optimal gravity-assist trajectories without a priori knowledge of the flyby-body combination. Since gravity assists are particularly crucial for reaching the outer Solar System, we use the Ice Giants, Uranus and Neptune, as example destinations for this work. Catalogs are also provided that list the most attractive trajectories found over launch dates ranging from 2024 to 2038. The tool developed to implement this method, called the Python EMTG Automated Trade Study Application (PEATSA), iteratively runs the Evolutionary Mission Trajectory Generator (EMTG), a NASA Goddard Space Flight Center in-house trajectory optimization tool. EMTG finds gravity-assist trajectories with impulsive maneuvers using a multiple-shooting structure along with stochastic methods (such as monotonic basin hopping) and may be run with or without an initial guess provided. PEATSA runs instances of EMTG in parallel over a grid of launch dates. After each set of runs completes, the best results within a neighborhood of launch dates are used to seed all other cases in that neighborhood---allowing the solutions across the range of launch dates to improve over each iteration. The results here are compared against trajectories found using a grid-search technique, and PEATSA is found to outperform the grid-search results for most launch years considered.

  5. Functional Fe-Pd nanomaterials synthesized by template-assisted methods

    Energy Technology Data Exchange (ETDEWEB)

    Prida, V.M., E-mail: vmpp@uniovi.es [Depto. de Fisica, Universidad de Oviedo, Calvo Sotelo s/n, 33007 Oviedo, Asturias (Spain); Vega, V.; Garcia, J.; Gonzalez, L.; Rosa, W.O.; Fernandez, A.; Hernando, B. [Depto. de Fisica, Universidad de Oviedo, Calvo Sotelo s/n, 33007 Oviedo, Asturias (Spain)

    2012-10-15

    In this work, we highlight our recent progress in the synthesis and characterization of functional nanomaterials based on Fe-Pd ferromagnetic alloys by means of template-assisted deposition techniques employing highly ordered nanoporous alumina membranes, such as ordered arrays of nanowires and antidots thin films. Special attention is paid on their basic magnetic properties, such as coercivity, remanence and magnetic anisotropy, and their dependence on the microstructure and morphological parameters of the ordered arrays.

  6. A comparative assessment of prosthetic outcome on enucleation and evisceration in three different etiological eye defects: A case series.

    Science.gov (United States)

    Jamayet, Nafij Bin; Kirangi, John Kariuki; Husein, Adam; Alam, Mohammad Khursheed

    2017-01-01

    Enucleation and evisceration are the most common surgical procedures that are performed to manage tumor, trauma, and infection. Given the consequences of surgical intervention, the conditions of the remaining eye socket may affect future prosthetic rehabilitation. A custom-made ocular prosthesis can be used to help restore the esthetics and functional defects and to improve the quality of life of patients with such conditions. An assessment must be performed on the prosthetic outcome before rehabilitation. The etiology of defect, type of surgery, condition of the remaining socket, and patient's age should all be considered. This report discusses three different etiological eye defects that have undergone enucleation and evisceration and describes the factors that have a significant role in the esthetic and functional outcome of the prosthesis. This report should serve as a helpful aid for maxillofacial prosthodontists to understand the primary objective of rehabilitating each eye defect and to meet patient expectations.

  7. Ankyrin and band 3 differentially affect expression of membrane glycoproteins but are not required for erythroblast enucleation.

    Science.gov (United States)

    Ji, Peng; Lodish, Harvey F

    2012-01-27

    During late stages of mammalian erythropoiesis the nucleus undergoes chromatin condensation, migration to the plasma membrane, and extrusion from the cytoplasm surrounded by a segment of plasma membrane. Since nuclear condensation occurs in all vertebrates, mammalian erythroid membrane and cytoskeleton proteins were implicated as playing important roles in mediating the movement and extrusion of the nucleus. Here we use erythroid ankyrin deficient and band 3 knockout mouse models to show that band 3, but not ankyrin, plays an important role in regulating the level of erythroid cell membrane proteins, as evidenced by decreased cell surface expression of glycophorin A in band 3 knockout mice. However, neither band 3 nor ankyrin are required for enucleation. These results demonstrate that mammalian erythroblast enucleation does not depend on the membrane integrity generated by the ankyrin-band 3 complex. Copyright © 2011 Elsevier Inc. All rights reserved.

  8. Learning curve for bipolar transurethral enucleation and resection of the prostate in saline for symptomatic benign prostatic hyperplasia: experience in the first 100 consecutive patients.

    Science.gov (United States)

    Xiong, Wei; Sun, Minghan; Ran, Qing; Chen, Fang; Du, Yangchun; Dou, Ke

    2013-01-01

    Bipolar transurethral enucleation and resection of the prostate in saline has recently been considered as a safe and technically feasible endoscopic procedure for symptomatic benign prostatic hyperplasia. However, it has not been accepted widely because of the perception of technical difficulty. A retrospective data review was performed of the first consecutive 100 patients who had undergone bipolar transurethral enucleation and resection of prostatic adenoma. Operative outcome, complications, ratio of conversion to conventional transurethral resection of the prostate and efficiency of tissue enucleation and resection were used to assess the learning curve. Bipolar transurethral enucleation and resection of the prostate was successfully performed in 83 patients. The mean operative time was 117.5 min, and the mean indwelling catheterization was 3.3 ± 1.9 days. After 6 months, maximum urinary flow was 21.34 ± 4.09 ml/s, IPSS was 9.66 ± 2.64, and quality of life was 2.31 ± 0.92 with a residual prostate volume of 35.29 ± 17.57 ml. Regarding the learning curve, the ratio of conversion to conventional bipolar transurethral resection of the prostate decreased after 30 cases, and the efficiency of enucleation and resection increased significantly with accumulative experience after 50 cases. The current results established that bipolar transurethral enucleation and resection of the prostate in saline is a safe and reproducible procedure. Copyright © 2012 S. Karger AG, Basel.

  9. Deployment of assistive living technology in a nursing home environment: methods and lessons learned.

    Science.gov (United States)

    Aloulou, Hamdi; Mokhtari, Mounir; Tiberghien, Thibaut; Biswas, Jit; Phua, Clifton; Kenneth Lin, Jin Hong; Yap, Philip

    2013-04-08

    With an ever-growing ageing population, dementia is fast becoming the chronic disease of the 21st century. Elderly people affected with dementia progressively lose their autonomy as they encounter problems in their Activities of Daily Living (ADLs). Hence, they need supervision and assistance from their family members or professional caregivers, which can often lead to underestimated psychological and financial stress for all parties. The use of Ambient Assistive Living (AAL) technologies aims to empower people with dementia and relieve the burden of their caregivers.The aim of this paper is to present the approach we have adopted to develop and deploy a system for ambient assistive living in an operating nursing home, and evaluate its performance and usability in real conditions. Based on this approach, we emphasise on the importance of deployments in real world settings as opposed to prototype testing in laboratories. We chose to conduct this work in close partnership with end-users (dementia patients) and specialists in dementia care (professional caregivers). Our trial was conducted during a period of 14 months within three rooms in a nursing home in Singapore, and with the participation of eight dementia patients and two caregivers. A technical ambient assistive living solution, consisting of a set of sensors and devices controlled by a software platform, was deployed in the collaborating nursing home. The trial was preceded by a pre-deployment period to organise several observation sessions with dementia patients and focus group discussions with professional caregivers. A process of ground truth and system's log data gathering was also planned prior to the trial and a system performance evaluation was realised during the deployment period with the help of caregivers. An ethical approval was obtained prior to real life deployment of our solution. Patients' observations and discussions allowed us to gather a set of requirements that a system for elders with

  10. Comparison of transurethral enucleation with bipolar and transurethral resection in saline for managing benign prostatic hyperplasia.

    Science.gov (United States)

    Hirasawa, Yosuke; Ide, Hiroki; Yasumizu, Yota; Hoshino, Katsura; Ito, Yujiro; Masuda, Takeshi

    2012-12-01

    Study Type--Therapy (case series) Level of Evidence 4. What's known on the subject? and What does the study add? Transurethral enucleation with bipolar (TUEB) has been widely adopted as an alternative to standard TURP, although the surgical technique, efficacy and safety of TUEB have not been described so far. The present study provides illustrations of the TUEB technique, as well as peri-operative, 6-month and 12-month follow-up results of TUEB from a retrospective review of a single-institution experience comparing the efficacy and safety of TUEB and transurethral resection in saline for managing BPH. • To illustrate the transurethral enucleation with bipolar (TUEB) technique and compare the efficacy and safety of TUEB and transurethral resection in saline (TURis) for managing benign prostatic hyperplasia (BPH). • A retrospective review of a single-institution experience of 110 consecutive TUEB or TURis between 2008 and 2011 at our hospital was performed aiming to compare the efficacy and safety of TUEB and TURis for managing BPH (55 patients in each group). • Peri-operative data included operating time, resected tissue weight, changes in haemoglobin, duration of catheterization, hospital stay and early complications. • Postoperative outcomes included 6- and 12-month postoperative International Prostate Symptom Score (IPSS), and Quality of Life (QoL) score, uroflowmetry findings, change in serum prostate-specific antigen level and all late complications in the two groups. • Both groups were well matched for age, preoperative prostate volume, IPSS, QoL and uroflowmetry findings. • The change in haemoglobin at postoperative day 1 in the TUEB group was significantly less than in the TURis group (1.08 vs 1.60 g/dL; P < 0.001). • Catheterization time and hospital stay were significantly shorter in the TUEB group than in the TURis group and the mean retrieved tissue weight in the TUEB group was larger than that in the TURis group (41.3 vs 31.7 g; P = 0

  11. Microwave-assisted multicomponent reactions for rapid synthesis of AIE-active fluorescent polymeric nanoparticles by post-polymerization method.

    Science.gov (United States)

    Cao, Qian-Yong; Jiang, Ruming; Liu, Meiying; Wan, Qing; Xu, Dazhuang; Tian, Jianwen; Huang, Hongye; Wen, Yuanqing; Zhang, Xiaoyong; Wei, Yen

    2017-11-01

    The development of simple and effective methods for synthesis of fluorescent polymeric nanoparticles (FPNs) with aggregation-induced emission (AIE) plays an important role for the biomedical applications of AIE-active FPNs. In present work, we developed a facile strategy for the fabrication of AIE-active FPNs by a post-polymerization method based on the microwave-assisted Kabachnik-Fields (KF) reaction, which can conjugate with poly(PEGMA-NH2), AIE-active dye (TPE-CHO) and diethyl phosphate (DP) under microwave irradiation within 5min. The characterization results confirm that PEGMA-TPE FPNs are successfully prepared through the microwave-assisted KF reaction. The resultant AIE-active FPNs show high water dispersity, intensive fluorescence and low cytotoxicity. These features make these AIE-active FPNs great potential for biomedical applications. Moreover, the microwave-assisted KF reaction is simple, fast, atom economy that should be a general strategy for the fabrication of various multifunctional AIE-active FPNs. We believe this work will open up a new avenue for the preparation of AIE-active functional materials with great potential for different applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Superiority of resection over enucleation for schwannomas of the cervical vagus nerve: A retrospective cohort study of 22 consecutive patients.

    Science.gov (United States)

    Illuminati, Giulio; Pizzardi, Giulia; Minni, Antonio; Masci, Federica; Ciamberlano, Bernardo; Pasqua, Rocco; Calio, Francesco G; Vietri, Francesco

    2016-05-01

    Schwannoma of the cervical vagus nerve is rare. Treatment options include intracapsular enucleation and en bloc resection. The purpose of this study was to compare the outcomes of enucleation and resection in terms of postoperative mortality and morbidity, freedom from vocal cord palsy, freedom from local recurrence, quality-adjusted life-year (QALY) and vocal handicap index (VHI). Twentytwo consecutive patients were divided into two groups. Patients in group A (n = 9) underwent intracapsular enucleation, whereas patients in Group B (n = 13) underwent en bloc resection. Main endpoints of the study were postoperative mortality and morbidity, freedom from vocal cord palsy, freedom from local recurrence and quality of life. The quality of life after surgery was assessed according to the quality-adjusted life-year (QALY) EQ-5D-5L methodology, and calculation of the voice handicap index (VHI). Postoperative mortality was nil. Morbidity included 1 wound dehiscence in group A and 2 transitory dysphagias in group B. Freedom from vocal cord palsy was 22% in group A and zero in group B (p = 0.15). Operation-specific local recurrence rate was 33% (3/9 patients) in group A and nil in group B (0/23 patients) (p = 0.05). QALYs was 0.55 in group A and 0.54 in group B (p = 1.0). VHI was 23.77 in group A and 26.15 in group B (p = 1.00). Resection is superior to enucleation in terms of freedom from local recurrence. Functional results are comparable for both techniques. Copyright © 2016 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.

  13. An Effective Vacuum Assisted Extraction Method for the Optimization of Labdane Diterpenoids from Andrographis paniculata by Response Surface Methodology

    Directory of Open Access Journals (Sweden)

    Ya-Qi Wang

    2014-12-01

    Full Text Available An effective vacuum assisted extraction (VAE technique was proposed for the first time and applied to extract bioactive components from Andrographis paniculata. The process was carefully optimized by response surface methodology (RSM. Under the optimized experimental conditions, the best results were obtained using a boiling temperature of 65 °C, 50% ethanol concentration, 16 min of extraction time, one extraction cycles and a 12:1 liquid-solid ratio. Compared with conventional ultrasonic assisted extraction and heat reflux extraction, the VAE technique gave shorter extraction times and remarkable higher extraction efficiency, which indicated that a certain degree of vacuum gave the solvent a better penetration of the solvent into the pores and between the matrix particles, and enhanced the process of mass transfer. The present results demonstrated that VAE is an efficient, simple and fast method for extracting bioactive components from A. paniculata, which shows great potential for becoming an alternative technique for industrial scale-up applications.

  14. Standard test method for determining a threshold stress intensity factor for environment-assisted cracking of metallic materials

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2003-01-01

    1.1 This test method covers the determination of the environment-assisted cracking threshold stress intensity factor parameters, KIEAC and KEAC, for metallic materials from constant-force testing of fatigue precracked beam or compact fracture specimens and from constant-displacement testing of fatigue precracked bolt-load compact fracture specimens. 1.2 This test method is applicable to environment-assisted cracking in aqueous or other aggressive environments. 1.3 Materials that can be tested by this test method are not limited by thickness or by strength as long as specimens are of sufficient thickness and planar size to meet the size requirements of this test method. 1.4 A range of specimen sizes with proportional planar dimensions is provided, but size may be variable and adjusted for yield strength and applied force. Specimen thickness is a variable independent of planar size. 1.5 Specimen configurations other than those contained in this test method may be used, provided that well-established stress ...

  15. Ankyrin and band 3 differentially affect expression of membrane glycoproteins but are not required for erythroblast enucleation

    Energy Technology Data Exchange (ETDEWEB)

    Ji, Peng, E-mail: peng-ji@fsm.northwestern.edu [Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611 (United States); Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (United States); Lodish, Harvey F. [Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (United States); Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States)

    2012-01-27

    Highlights: Black-Right-Pointing-Pointer Ankyrin and band 3 are not required for erythroblasts enucleation. Black-Right-Pointing-Pointer Loss of ankyrin does not affect erythroid membrane glycoprotein expression. Black-Right-Pointing-Pointer Loss of band 3 influences erythroid membrane glycoprotein expression. -- Abstract: During late stages of mammalian erythropoiesis the nucleus undergoes chromatin condensation, migration to the plasma membrane, and extrusion from the cytoplasm surrounded by a segment of plasma membrane. Since nuclear condensation occurs in all vertebrates, mammalian erythroid membrane and cytoskeleton proteins were implicated as playing important roles in mediating the movement and extrusion of the nucleus. Here we use erythroid ankyrin deficient and band 3 knockout mouse models to show that band 3, but not ankyrin, plays an important role in regulating the level of erythroid cell membrane proteins, as evidenced by decreased cell surface expression of glycophorin A in band 3 knockout mice. However, neither band 3 nor ankyrin are required for enucleation. These results demonstrate that mammalian erythroblast enucleation does not depend on the membrane integrity generated by the ankyrin-band 3 complex.

  16. Benign Prostatic Hyperplasia Treatment by Transurethral Enucleation of the Prostate Using a 2-μm Laser.

    Science.gov (United States)

    Guo, He-Qing; Zhou, Gao-Biao; Liu, Hong-Ming; Sun, Bin; Pan, Guang-Xin; Mu, Da-Wei; Yan, Jing-Ming; Xing, Ji-Zhang; Li, Di; Hong, Quan

    2015-12-01

    This study investigates the efficacy of benign prostatic hyperplasia (BPH) treatment by prostate transurethral enucleation using a 2-μm laser. A total of 107 patients with BPH were treated by prostate transurethral enucleation using a RevoLix 2-μm laser surgery system. Bleeding volume, operation time, catheterization time, voiding situation, maximum urinary flow rate, and hospital stay were observed. The mean operation time was 74 min ± 12 min (range 45 to 150 min), the mean follow-up period was 2 to 6 months, the mean catheter time was 5 days, and the mean peak urinary flow rate increased from 6.3 ± 0.6 to 17.5 ± 1.5 mL/s. The International Prostate Symptom Score and quality of life significantly declined (p transurethral enucleation using a 2-μm laser is safe and efficient for BPH treatment.

  17. Melanocortins contribute to sequential differentiation and enucleation of human erythroblasts via melanocortin receptors 1, 2 and 5.

    Directory of Open Access Journals (Sweden)

    Eriko Simamura

    Full Text Available In this study, we showed that adrenocorticotropic hormone (ACTH promoted erythroblast differentiation and increased the enucleation ratio of erythroblasts. Because ACTH was contained in hematopoietic medium as contamination, the ratio decreased by the addition of anti-ACTH antibody (Ab. Addition of neutralizing Abs (nAbs for melanocortin receptors (MCRs caused erythroblast accumulation at specific stages, i.e., the addition of anti-MC2R nAb led to erythroblast accumulation at the basophilic stage (baso-E, the addition of anti-MC1R nAb caused accumulation at the polychromatic stage (poly-E, and the addition of anti-MC5R nAb caused accumulation at the orthochromatic stage (ortho-E. During erythroblast differentiation, ERK, STAT5, and AKT were consecutively phosphorylated by erythropoietin (EPO. ERK, STAT5, and AKT phosphorylation was inhibited by blocking MC2R, MC1R, and MC5R, respectively. Finally, the phosphorylation of myosin light chain 2, which is essential for the formation of contractile actomyosin rings, was inhibited by anti-MC5R nAb. Taken together, our study suggests that MC2R and MC1R signals are consecutively required for the regulation of EPO signal transduction in erythroblast differentiation, and that MC5R signal transduction is required to induce enucleation. Thus, melanocortin induces proliferation and differentiation at baso-E, and polarization and formation of an actomyosin contractile ring at ortho-E are required for enucleation.

  18. Performance evaluation of 3D vision-based semi-autonomous control method for assistive robotic manipulator.

    Science.gov (United States)

    Ka, Hyun W; Chung, Cheng-Shiu; Ding, Dan; James, Khara; Cooper, Rory

    2017-03-22

    We developed a 3D vision-based semi-autonomous control interface for assistive robotic manipulators. It was implemented based on one of the most popular commercially available assistive robotic manipulator combined with a low-cost depth-sensing camera mounted on the robot base. To perform a manipulation task with the 3D vision-based semi-autonomous control interface, a user starts operating with a manual control method available to him/her. When detecting objects within a set range, the control interface automatically stops the robot, and provides the user with possible manipulation options through audible text output, based on the detected object characteristics. Then, the system waits until the user states a voice command. Once the user command is given, the control interface drives the robot autonomously until the given command is completed. In the empirical evaluations conducted with human subjects from two different groups, it was shown that the semi-autonomous control can be used as an alternative control method to enable individuals with impaired motor control to more efficiently operate the robot arms by facilitating their fine motion control. The advantage of semi-autonomous control was not so obvious for the simple tasks. But, for the relatively complex real-life tasks, the 3D vision-based semi-autonomous control showed significantly faster performance. Implications for Rehabilitation A 3D vision-based semi-autonomous control interface will improve clinical practice by providing an alternative control method that is less demanding physically as well cognitively. A 3D vision-based semi-autonomous control provides the user with task specific intelligent semiautonomous manipulation assistances. A 3D vision-based semi-autonomous control gives the user the feeling that he or she is still in control at any moment. A 3D vision-based semi-autonomous control is compatible with different types of new and existing manual control methods for ARMs.

  19. Orbital and Subcutaneous Emphysema Following Enucleation and Respiratory Distress in a Japanese Chin.

    Science.gov (United States)

    Gornik, Kara R; Pirie, Christopher G; Alario, Anthony F

    2015-01-01

    A 7 yr old, neutered male Japanese chin presented to the Cummings School of Veterinary Medicine at Tufts University (CSVMTU) for evaluation of chronic unilateral orbital swelling that worsened following an episode of respiratory distress. The left eye had been enucleated 5 yr previously. Intermittent mild-to-moderate left orbital swelling had been noted by the owner since the initial surgery. Examination demonstrated a moderate-to-severe, soft, fluctuant swelling involving the left orbit with erythema of the overlying skin. Crepitus was noted over the occipital tuberosity. Computed tomography revealed a large volume of gas involving the left orbit. The gas extended caudally within the subcutaneous tissues to both hemimandibles, dorsal to the cranium, and partially surrounded the cranial neck. The presence of a mucosa-lined, air-filled space with a patent nasolacrimal duct was noted on orbital exploration. The lining was removed and the duct closed. Histopathology confirmed the presence of an epithelial lining. No recurrence of the swelling was observed on examination 8 wk after surgery. This is the first report documenting acute worsening of orbital swelling following an episode of respiratory distress. This case highlights the importance of addressing the nasolacrimal duct while performing an enculeation in a brachycephalic dog.

  20. Histopathology of enucleated intraocular melanomas irradiated with cobalt and ruthenium plaques

    Energy Technology Data Exchange (ETDEWEB)

    Fuchs, U.; Kivelae, T.; Tarkkanen, A.; Laatikainen, L.

    1988-01-01

    Eight malignant uveal melanomas irradiated with Stallard's /sup 60/Co applicator or Lommatzsch's /sup 106/Ru//sup 10/ /sup 6/Rh applicator were studied by light microscopy and immunohistochemistry to analyse tumour cell morphology, antigenic expression and inflammatory cell infiltration in relation to radiation dose. The tumours were enucleated 1 to 24 months after irradiation because they did not regress or continued to grow. One patient has later died of metastatic disease. Eight uveal melanomas that were not irradiated were studied for comparative purposes. Radiation necrosis was found only in two cases. In four irradiated and in one control melanoma conspicuous collagen fibres were present in the basal part of the tumour. No difference was noted between the two groups in the number of mitotic figures or in the morphology of tumour cells. In all cases but one, more than half of the tumour cells were variably positive for S-100 protein. Neutron-specific enolase-positive melanoma cells were few in number and occurred in small clusters, and were somewhat more common in irradiated cases. A few lymphocytes and macrophages, which were preferentially found near the choroid, infiltrated every melanoma, but only single cells were associated with radiation necrosis. A mild scleritis near the tumour was present irrespective of irradiation. Although the melanomas were quite large for irradiation, the lack of necrosis in optimally irradiated basal parts of the tumour additionally indicates substantial radioresistance.

  1. Deep Eutectic Solvent-Based Microwave-Assisted Method for Extraction of Hydrophilic and Hydrophobic Components from Radix Salviae miltiorrhizae

    Directory of Open Access Journals (Sweden)

    Jue Chen

    2016-10-01

    Full Text Available Deep eutectic solvents (DESs have attracted significant attention as a promising green media. In this work, twenty-five kinds of benign choline chloride-based DESs with microwave-assisted methods were applied to quickly extract active components from Radix Salviae miltiorrhizae. The extraction factors, including temperature, time, power of microwave, and solid/liquid ratio, were investigated systematically by response surface methodology. The hydrophilic and hydrophobic ingredients were extracted simultaneously under the optimized conditions: 20 vol% of water in choline chloride/1,2-propanediol (1:1, molar ratio as solvent, microwave power of 800 W, temperature at 70 °C, time at 11.11 min, and solid/liquid ratio of 0.007 g·mL−1. The extraction yield was comparable to, or even better than, conventional methods with organic solvents. The microstructure alteration of samples before and after extraction was also investigated. The method validation was tested as the linearity of analytes (r2 > 0.9997 over two orders of magnitude, precision (intra-day relative standard deviation (RSD < 2.49 and inter-day RSD < 2.96, and accuracy (recoveries ranging from 95.04% to 99.93%. The proposed DESs combined with the microwave-assisted method provided a prominent advantage for fast and efficient extraction of active components, and DESs could be extended as solvents to extract and analyze complex environmental and pharmaceutical samples.

  2. A New Celestial Navigation Method for Spacecraft on a Gravity Assist Trajectory

    OpenAIRE

    Ning Xiaolin; Huang Panpan; Fang Jiancheng

    2013-01-01

    A practical and reliable capability for autonomous navigation needs to reduce operation cost, to improve operational efficiency, and to increase mission safety. Celestial navigation is a very attractive autonomous navigation solution for deep space spacecraft. There are mainly two kinds of celestial navigation methods: the direct calculation method and the filter method. The accuracy of the direct calculation method is low and very sensitive to the measurement noise. The filter method can pro...

  3. Assessing symmetry using the mirror stand device with manual and software-assisted methods in postoperative zygomatic fracture patients

    Science.gov (United States)

    Syarif, A. N.; Bangun, K.

    2017-08-01

    Zygomatic fractures are among the most common fractures to the facial skeleton. However, because no standard and reliable method of evaluation is available to assess postoperative patients, we often rely on photographs and subjective assessments. A portable mirror stand device (MiRS), which is a new method for the standardization of photography, was developed in our institution. Used with image analysis software, this device provides a new method for evaluating outcomes after the open reduction and internal fixation of zygomatic fractures. The portable mirror stand device was set up in our outpatient clinic at the Cleft Craniofacial Center at Cipto Mangunkusumo Hospital. Photographs of 11 postoperative patients were taken using the device, and they were analyzed both manually and using image analysis software (ImageJ 1.46) for symmetry. The two methods were then compared to assess the correlation and agreement of the results. The measurements taken using the manual method and the software-assisted method did not differ significantly, which indicated the good agreement between the two methods. The results of the symmetry achieved atour center were similar to other centers in the Asian region (ΔZy = 3.4±1.5 mm, ΔBc = 2.6±1.6 mm, ΔCh = 2.3±2.4 mm) compared with (ΔZy = 3.2±1.7 mm, ΔBc = 2.6±1.6 mm, ΔCh = 2.3±2.5 mm). The treatment of zygomatic fracture a tour center achieved good results. The portable mirror stand device assisted the image analysis software (ImageJ 1.46), which could be beneficial in assessing symmetry in postoperative zygomatic fracture patients.

  4. High performance flexible metal oxide/silver nanowire based transparent conductive films by a scalable lamination-assisted solution method

    Directory of Open Access Journals (Sweden)

    Hua Yu

    2017-03-01

    Full Text Available Flexible MoO3/silver nanowire (AgNW/MoO3/TiO2/Epoxy electrodes with comparable performance to ITO were fabricated by a scalable solution-processed method with lamination assistance for transparent and conductive applications. Silver nanoparticle-based electrodes were also prepared for comparison. Using a simple spin-coating and lamination-assisted planarization method, a full solution-based approach allows preparation of AgNW-based composite electrodes at temperatures as low as 140 °C. The resulting flexible AgNW-based electrodes exhibit higher transmittance of 82% at 550 nm and lower sheet resistance about 12–15 Ω sq−1, in comparison with the values of 68% and 22–25 Ω sq−1 separately for AgNP based electrodes. Scanning electron microscopy (SEM and Atomic force microscopy (AFM reveals that the multi-stacked metal-oxide layers embedded with the AgNWs possess lower surface roughness (<15 nm. The AgNW/MoO3 composite network could enhance the charge transport and collection efficiency by broadening the lateral conduction range due to the built of an efficient charge transport network with long-sized nanowire. In consideration of the manufacturing cost, the lamination-assisted solution-processed method is cost-effective and scalable, which is desire for large-area fabrication. While in view of the materials cost and comparable performance, this AgNW-based transparent and conductive electrodes is potential as an alternative to ITO for various optoelectronic applications.

  5. Guidestar-assisted wavefront-shaping methods for focusing light into biological tissue

    Science.gov (United States)

    Horstmeyer, Roarke; Ruan, Haowen; Yang, Changhuei

    2015-09-01

    In the field of biomedical optics, optical scattering has traditionally limited the range of imaging within tissue to a depth of one millimetre. A recently developed class of wavefront-shaping techniques now aims to overcome this limit and achieve diffraction-limited control of light beyond one centimetre. By manipulating the spatial profile of an optical field before it enters a scattering medium, it is possible to create a micrometre-scale focal spot deep within tissue. To successfully operate in vivo, these wavefront-shaping techniques typically require feedback from within the biological sample. This Review summarizes recently developed 'guidestar' mechanisms that provide feedback for intra-tissue focusing. Potential applications of guidestar-assisted focusing include optogenetic control over neurons, targeted photodynamic therapy and deep tissue imaging.

  6. Comparison of techniques for transurethral laser prostatectomy: standard photoselective vaporization of the prostate versus transurethral laser enucleation of the prostate.

    Science.gov (United States)

    Elterman, Dean S; Chughtai, Bilal; Lee, Richard; Kurlander, Lauren; Yip-Bannicq, Marika; Kaplan, Steven A; Te, Alexis E

    2013-06-01

    Transurethral laser enucleation of the prostate (TLEP) using the potassium-titanyl-phosphate (KTP) laser offers an alternative technique to traditional photovaporization. The study objective was to determine the comparative efficacy between transurethral photovaporization of the prostate (PVP) with a TLEP technique using the 80W 532 nm KTP laser. A series of 97 vs 170 patients who underwent PVP vs TLEP, respectively, with the KTP laser system at Weill Cornell Medical College from September 2001 to May 2009 was studied retrospectively. Outcome measures included laser time, prostate volume lased per unit time, International Prostate Symptom Score (IPSS), postvoid residual (PVR), and maximum flow rate (Qmax). Statistical analyses were performed using the Shapiro-Wilk, Mann-Whitney, Wilcoxon, and unpaired t tests. Baseline parameters were similar between groups, although volume was greater in the TLEP group (83 vs 63 cc, P=0.04). Median laser time was longer in the TLEP group (90 vs 50 min, P<0.001) with a higher median energy used (308 vs 165 kJ, P<0.001). The volume lased per unit time was shorter, however, for TLEP (0.92 cc/min) than for PVP (1.26 cc/min). A greater median number of fibers were used in TLEP (2.5 vs 2.0, P=0.001). Improvements in median IPSS and PVR were seen in the TLEP group (5.0, P<0.001; 55.5, P=0.02, respectively) but not in the PVP group (P=0.40 and 0.30). Median Qmax and prostate-specific antigen (PSA) level improved similarly in both groups. Final IPSS was lower for the TLEP group (P<0.001), but other final parameters were statistically equivalent. In our series, both PVP and TLEP techniques were safe and effective. Although changes in Qmax and PSA were similar between the two techniques, improvement in urinary symptoms and PVR was superior with the TLEP technique. The TLEP technique was a more efficient method for laser prostatectomy.

  7. Source Allocation of Long-Range Asian Dusts Transportation across the Taiwan Strait by Innovative Chemical-Assisted Identification Methods

    Directory of Open Access Journals (Sweden)

    Yi-Hsiu Jen

    2014-01-01

    Full Text Available This study used the backward trajectory calculation to obtain the transportation routes of Asian dusts and further combined the chemical composition with the enrichment factor (EF and the grey relational analysis (GR to identify the potential sources of eighteen Asian dust storm (ADS events. The results showed that the chemical compositions of atmospheric particles sampled at the Pescadores Islands were very similar to source soils fugitively emitted from Inner Mongolia, which could assist in identifying the source regions of Asian dusts. This study further compared the source allocation of Asian dusts obtained from EF, GR, and backward trajectory, which showed that the source regions of Asian dusts obtained from these three methods were quite similar. The similarity of backward trajectory and GR reached as high as 83.3%. Moreover, the similarity of backward trajectory calculation and EF or GR was up to 77.8% while that of the GR and EF was up to 83.3%. Overall, these three methods can successfully allocate the source regions of Asian dusts by 66.7%. Moreover, these innovative chemical-assisted methods can be successfully applied to identify the source regions of Asian dusts for 18 ADS events.

  8. Hierarchical porous carbon derived from Allium cepa for supercapacitors through direct carbonization method with the assist of calcium acetate

    KAUST Repository

    Xu, Jinhui

    2017-11-02

    In this paper, a direction carbonization method was used to prepare porous carbon from Allium cepa for supercapacitor applications. In this method, calcium acetate was used to assist carbonization process. Scanning electron microscope (SEM) and N2 adsorption/desorption method were used to characterize the morphology, Brunauer-Emmett-Teller (BET) specific surface area and pore size distribution of porous carbon derived from Allium cepa (onion derived porous carbon, OPC). OPC is of hierarchical porous structure with high specific surface area and relatively high specific capacitance. OPC possesses relatively high specific surface area of 533.5 m2/g. What’s more, OPC possesses a specific capacitance of 133.5 F/g at scan rate of 5 mV/s.

  9. Effect of onion extract on corneal haze suppression after air assisted lamellar keratectomy

    Science.gov (United States)

    KIM, Soohyun; PARK, Young Woo; LEE, Euiri; PARK, Sang Wan; PARK, Sungwon; NOH, Hyunwoo; KIM, Jong Whi; SEONG, Je Kyung; SEO, Kangmoon

    2015-01-01

    This study evaluated the effect of onion extract on corneal haze suppression after applying the air assisted lamellar keratectomy. The air assisted lamellar keratectomy was performed on 24 canine eyes. They were treated with an artificial tear (group C), prednisolone acetate (group P), onion extract (group O) and TGF-β1 (group T) three times per day from 7 to 28 days after the surgery. Corneal haze occurred on the all eyes and was observed beginning 7 days after the surgery. The haze was significantly decreased in groups P and O from day 14 compared with the group C using the clinical (group P; P=0.021, group O; P=0.037) and objective evaluation method (group P; P=0.021, group O; P=0.039). In contrast, it was significantly increased in group T from day 14 compared with group C based on the clinical (P=0.002) and objective evaluation method (P<0.001). Subsequently, these eyes were enucleated after euthanasia, and immunohistochemistry with α-SMA antibodies was done. The total green intensity for α-SMA was significantly more expressed in group T and significantly less expressed in groups P and O than in group C. Onion extract could have potential as a therapeutic in preventing corneal haze development by suppressing the differentiation of fibroblasts into myofibroblasts. PMID:26607134

  10. Probing the effect of human normal sperm morphology rate on cycle outcomes and assisted reproductive methods selection.

    Directory of Open Access Journals (Sweden)

    Bo Li

    Full Text Available Sperm morphology is the best predictor of fertilization potential, and the critical predictive information for supporting assisted reproductive methods selection. Given its important predictive value and the declining reality of semen quality in recent years, the threshold of normal sperm morphology rate (NSMR is being constantly corrected and controversial, from the 4th edition (14% to the 5th version (4%. We retrospectively analyzed 4756 cases of infertility patients treated with conventional-IVF(c-IVF or ICSI, which were divided into three groups according to NSMR: ≥14%, 4%-14% and <4%. Here, we demonstrate that, with decrease in NSMR(≥14%, 4%-14%, <4%, in the c-IVF group, the rate of fertilization, normal fertilization, high-quality embryo, multi-pregnancy and birth weight of twins gradually decreased significantly (P<0.05, while the miscarriage rate was significantly increased (p<0.01 and implantation rate, clinical pregnancy rate, ectopic pregnancy rate, preterm birth rate, live birth rate, sex ratio, and birth weight(Singleton showed no significant change. In the ICSI group, with decrease in NSMR (≥14%, 4%-14%, <4%, high-quality embryo rate, multi-pregnancy rate and birth weight of twins were gradually decreased significantly (p<0.05, while other parameters had no significant difference. Considering the clinical assisted methods selection, in the NFMR ≥14% group, normal fertilization rate of c-IVF was significantly higher than the ICSI group (P<0.05, in the 4%-14% group, birth weight (twins of c-IVF were significantly higher than the ICSI group, in the <4% group, miscarriage of IVF was significantly higher than the ICSI group. Therefore, we conclude that NSMR is positively related to embryo reproductive potential, and when NSMR<4% (5th edition, ICSI should be considered first, while the NSMR≥4%, c-IVF assisted reproduction might be preferred.

  11. Zygomatic surface marker-assisted surgical navigation: a new computer-assisted navigation method for accurate treatment of delayed zygomatic fractures.

    Science.gov (United States)

    He, Yang; Zhang, Yi; An, Jin-gang; Gong, Xi; Feng, Zhi-qiang; Guo, Chuan-bin

    2013-12-01

    To describe a new method of zygomatic surface marker navigation to treat delayed unilateral zygomatic fractures. The computed tomography (CT) data for 6 patients were obtained before surgery and imported into the surgical planning software. After 3-dimensional (3D) construction and segmentation, 3D cylindrical-shaped objects in stereolithographic format were placed in position and merged with the data from the fractured segments to mark the area for surface reduction. Data from the unaffected side were used to guide the reduction data for the segments with markers. During surgery, the surface markers were marked by drilling holes in the fractured bones in a process guided by the surgical navigation plan established before osteotomy. The segments were then reduced to the predetermined places using the positions of the hole markers as guides. 3D image comparisons and axial CT measurements were used to evaluate navigation accuracy and bone symmetry. Six patients with unilateral delayed zygomatic fractures were treated using this approach. The mean deviation between the postoperative 3D images and the reduction navigation plan for the 6 patients was +1.24 mm and -1.4 mm. The mean width deviation between the affected and unaffected sides was 1.28 mm, and the mean eminence deviation was 1.22 mm. All patients were followed up for at least 3 months and experienced no obvious complications. Zygomatic surface marker-assisted surgical navigation can simplify the navigation planning for surgery and avoid the complex protocols needed to create the surgical templates. The navigation accuracy was acceptable, and all 6 patients obtained good facial symmetry. Copyright © 2013. Published by Elsevier Inc.

  12. Malnutrition in older adults on financial assistance in an urban Asian country: a mixed methods study.

    Science.gov (United States)

    Koo, Yu Xuan; Kang, Min Li; Auyong, Angie; Liau, Glen Zq; Hoe, Jeremy; Long, Melody; Koh, Aaron; Koh, Frederick; Liu, Rain; Koh, Gerald

    2014-12-01

    The objectives of the present study were to assess the nutritional status, identify factors for malnutrition risk and evaluate barriers to adequate nutrition among recipients of the Public Assistance (PA) scheme for socio-economically disadvantaged Singaporeans. Using a cross-sectional study design, we assessed PA recipients' malnutrition risk using the DETERMINE Nutritional Health checklist and the full Mini-Nutritional Assessment (MNA), as well as their nutritional knowledge, co-morbidity burden, depression risk, instrumental and basic activities of daily living (IADL and BADL), and awareness and utilization of available food services. In-depth interviews were also conducted on malnourished individuals (MNA score 75 years, currently unmarried, BADL impairment, depression risk and BMI analysis revealed that financial, social and physical barriers and lack of knowledge were the main contributors to poor nutritional status. Only half were aware of subsidized food services and education increased interest in utilizing food services. Among nursing home respondents, those who were BADL impaired were more likely to be at risk of malnutrition. Among PA recipients, the prevalence of malnutrition is low but the risk of malnutrition is high. Education on adequate nutrition and food services are recommended.

  13. Ultrasound assisted method to increase soluble chemical oxygen demand (SCOD) of sewage sludge for digestion.

    Science.gov (United States)

    Grönroos, Antti; Kyllönen, Hanna; Korpijärvi, Kirsi; Pirkonen, Pentti; Paavola, Teija; Jokela, Jari; Rintala, Jukka

    2005-01-01

    The aim of this study was to clarify the possibilities to increase the amount of soluble chemical oxygen demand (SCOD) and methane production of sludge using ultrasound technologies with and without oxidising agents. The study was done using multivariate data analyses. The most important factors affected were discovered. Ultrasonically assisted disintegration increased clearly the amount of SCOD of sludge. Also more methane was produced from treated sludge in anaerobic batch assays compared to the sludge with no ultrasonic treatment. Multivariate data analysis showed that ultrasonic power, dry solid content of sludge (DS), sludge temperature and ultrasonic treatment time have the most significant effect on the disintegration. It was also observed that in the reactor studied energy efficiency with high ultrasound power together with short treatment time was higher than with low ultrasound power with long treatment time. When oxidising agents were used together with ultrasound no increase in SCOD was achieved compared the ultrasonic treatment alone and only a slight increase in total organic carbon of sludge was observed. However, no enhancement in methane production was observed when using oxidising agents together with ultrasound compared the ultrasonic treatment alone. Ultrasound propagation is an important factor in ultrasonic reactor scale up. Ultrasound efficiency rose linearly with input power in sludge at small distances from the transducer. Instead, ultrasound efficiency started even to decrease with input power at long distances from the transducer.

  14. Computer-assisted assessment of ocular synkinesis: a comparison of methods

    NARCIS (Netherlands)

    Kleiss, I.J.; Hohman, M.H.; Quatela, O.E.; Marres, H.A.M.; Hadlock, T.A.

    2013-01-01

    OBJECTIVES/HYPOTHESIS: To define an objective, easy to perform, rapid method for the assessment of ocular synkinesis by employing both the Facial Assessment by Computer Evaluation software and a modification of the Glasgow Facial Palsy Scale. STUDY DESIGN: Retrospective study. METHODS: Fifty normal

  15. An Illustration to Assist in Comparing and Remembering Several Multiplicity Adjustment Methods

    Science.gov (United States)

    Hasler, Mario

    2017-01-01

    There are many well-known or new methods to adjust statistical tests for multiplicity. This article provides an illustration helping lecturers or consultants to remember the differences of three important multiplicity adjustment methods and to explain them to non-statisticians.

  16. Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods.

    Science.gov (United States)

    Xu, Dong-Ping; Zheng, Jie; Zhou, Yue; Li, Ya; Li, Sha; Li, Hua-Bin

    2017-02-15

    Natural antioxidants are widely used as dietary supplements or food additives. An optimized method of ultrasound-assisted extraction (UAE) was proposed for the effective extraction of antioxidants from the flowers of Limonium sinuatum and evaluated by response surface methodology. In this study, ethanol concentration, ratio of solvent to solid, ultrasonication time and temperature were investigated and optimized using a central composite rotatable design. The optimum extraction conditions were as follows: ethanol concentration, 60%; ratio of solvent to solid, 56.9:1mL/g; ultrasonication time, 9.8min; and temperature, 40°C. Under the optimal UAE conditions, the experimental values (483.01±15.39μmolTrolox/gDW) matched with those predicted (494.13μmolTrolox/gDW) within a 95% confidence level. In addition, the antioxidant activities of UAE were compared with those of conventional maceration and Soxhlet extraction methods, and the ultrasound-assisted extraction could give higher yield of antioxidants and markedly reduce the extraction time. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. A new matrix assisted ionization method for the analysis of volatile and nonvolatile compounds by atmospheric probe mass spectrometry.

    Science.gov (United States)

    Chakrabarty, Shubhashis; Pagnotti, Vincent S; Inutan, Ellen D; Trimpin, Sarah; McEwen, Charles N

    2013-07-01

    Matrix assisted ionization of nonvolatile compounds is shown not to be limited to vacuum conditions and does not require a laser. Simply placing a solution of analyte dissolved with a suitable matrix such as 3-nitrobenzonitrile (3-NBN) or 2,5-dihydroxyacetophenone on a melting point tube and gently heating the dried sample near the ion entrance aperture of a mass spectrometer using a flow of gas produces abundant ions of peptides, small proteins, drugs, and polar lipids. Fundamental studies point to matrix-mediated ionization occurring prior to the entrance aperture of the mass spectrometer. The method is analytically useful, producing peptide mass fingerprints of bovine serum albumin tryptic digest consuming sub-picomoles of sample. Application of 100 fmol of angiotensin I in 3-NBN matrix produces the doubly and triply protonated molecular ions as the most abundant peaks in the mass spectrum. No carryover is observed for samples containing up to 100 pmol of this peptide. A commercial atmospheric samples analysis probe provides a simple method for sample introduction to an atmospheric pressure ion source for analysis of volatile and nonvolatile compounds without using the corona discharge but using sample preparation similar to matrix-assisted laser desorption/ionization.

  18. Optimization and Comparison of Ultrasound-Assisted Extraction of Estragole from Tarragon Leaves with Hydro-Distillation Method

    Directory of Open Access Journals (Sweden)

    Mohammad Bagher Gholivand

    2014-12-01

    Full Text Available A comparative study of ultrasound-assisted extraction (UAE and hydro-distillation was performed for fast extraction of estragole from tarragon (Artemisia dracunculus L. dried leaves. Several influential parameters of the UAE procedure in the extraction of estragole (type of solvent, extraction cycles, solvent to material ratio, irradiation time and particle size were investigated and optimized. It was found that UAE offers a more rapid extraction of estragole than hydrodistillation. The optimum parameters were solvent to material ratio of 8:1 v/m, 96% (w/w ethanol in water as extraction solvent, particle size of 1.18 mm, irradiation time of 5 min, output power of 63 W, 9 pulses, and ultrasonic frequency of 20 kHz. The recovery of estragole by UAE under optimal conditions was 44.4% based on dry extract. The benefit of ultrasound was to decrease the extraction time (5 min relative to the classical hydrodistillation method (3 h. The experimental results also indicated that ultrasound-assisted extraction is a simple, rapid and effective method for extraction of the volatile oil components of tarragon.

  19. Robotic Assistance Enables Inexperienced Surgeons to Perform Unicompartmental Knee Arthroplasties on Dry Bone Models with Accuracy Superior to Conventional Methods

    Directory of Open Access Journals (Sweden)

    Monil Karia

    2013-01-01

    Full Text Available Robotic systems have been shown to improve unicompartmental knee arthroplasty (UKA component placement accuracy compared to conventional methods when used by experienced surgeons. We aimed to determine whether inexperienced UKA surgeons can position components accurately using robotic assistance when compared to conventional methods and to demonstrate the effect repetition has on accuracy. Sixteen surgeons were randomised to an active constraint robot or conventional group performing three UKAs over three weeks. Implanted component positions and orientations were compared to planned component positions in six degrees of freedom for both femoral and tibial components. Mean procedure time decreased for both robot (37.5 mins to 25.7 mins (P=0.002 and conventional (33.8 mins to 21.0 mins (P=0.002 groups by attempt three indicating the presence of a learning curve; however, neither group demonstrated changes in accuracy. Mean compound rotational and translational errors were lower in the robot group compared to the conventional group for both components at all attempts for which rotational error differences were significant at every attempt. The conventional group’s positioning remained inaccurate even with repeated attempts although procedure time improved. In comparison, by limiting inaccuracies inherent in conventional equipment, robotic assistance enabled surgeons to achieve precision and accuracy when positioning UKA components irrespective of their experience.

  20. Assisted history matching using direct search optimization methods; Ajuste de historico assistido usando metodos de otimizacao de busca direta

    Energy Technology Data Exchange (ETDEWEB)

    Maschio, Celio; Schiozer, Denis J. [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia Mecanica. Dept. de Engenharia de Petroleo

    2004-07-01

    The goal of history matching is the improvement of reservoir characterization process through the incorporation of dynamic data in the process, that is, production and pressure data measured during well operation. The objective is the building of consistent simulation models, for to reproduce the observed data and to allow more reliable forecasting. History matching is performed under consistent changes of the reservoir properties, in order to decrease the distance between observed and simulated data. Manual process is a difficult and a tedious task and involves several trial and error steps and automatic or semi automatic history matching methods (assisted history matching) normally requires a large number of simulations. The purpose of the present work is the improvement of an optimization algorithm, based on direct search on grid space (using discretization of the properties), in order to accelerate the assisted history matching process. The algorithm uses a combination of exploratory and linear search to find the minimum of the objective function. The performance of the algorithm is evaluated through the comparison with an existing search methodology, using as parameters the number of simulation and the quality of the matching. The method is applied to a synthetic model and to a real field from Campos basin. The results show that is possible to reduce the number of simulation, preserving the quality of the matching. (author)

  1. Sexual outcome of patients undergoing thulium laser enucleation of the prostate for benign prostatic hyperplasia

    Directory of Open Access Journals (Sweden)

    Luca Carmignani

    2015-01-01

    Full Text Available Treatment of patients with lower urinary tract symptoms (LUTS secondary to benign prostatic hyperplasia (BPH may affect the quality of sexual function and ejaculation. The effect of new surgical procedures, which are currently available to treat BPH, on erection and ejaculation, has been poorly studied. This study aimed to assess the effect of thulium laser enucleation of the prostate (ThuLEP on sexual function and retrograde ejaculation in patients with LUTS secondary to BPH. We performed a prospective study in 110 consecutive patients who had undergone ThuLEP to analyze changes in sexual function and urinary symptoms. To evaluate changes in erection and ejaculation, and the effect of urinary symptoms on the quality of life (QoL, five validated questionnaires were used: the ICIQ-MLUTSsex, MSHQ-EjD, International Index of Erectile Function 5, International Prognostic Scoring System (IPSS questionnaire, and QoL index of the intraclass correlation coefficients. Patients also underwent IPSS and flowmetry to assess the outcome of flow. Patients were evaluated before surgery and 3-6 months after ThuLEP, whereas those with previous abdominal surgery were excluded. The patients′ mean age was 67.83 years. Postoperative urinary symptoms improved after surgery. No significant differences in erectile function before and after surgery were observed. As compared with other techniques described in the literature, the percentage of patients with conserved ejaculation increased by 52.7% after ThuLEP. ThuLEP positively affects urinary symptoms and their effect on the QoL of patients as assessed by questionnaire scores. While endoscopic management of BPH (e.g. transurethral resection of the prostate causes retrograde ejaculation in most patients, those who undergo ThuLEP have conserved ejaculation and erectile function.

  2. Holmium laser enucleation of the prostate versus transurethral resection of the prostate: a randomized clinical trial.

    Science.gov (United States)

    Sun, Nao; Fu, Yaowen; Tian, Tengzheng; Gao, Jialin; Wang, Yuantao; Wang, Song; An, Wei

    2014-07-01

    To evaluate the efficacy and safety of holmium laser enucleation of the prostate (HoLEP) and transurethral resection of the prostate (TURP), for treatment of benign prostatic hyperplasia (BPH). A total of 164 cases of BPH were selected from patients who were hospitalized between January 2010 and December 2011. Patients had received either HoLEP or TURP treatment. Clinical data were collected from the perioperative period, 1 month after surgery, and 12 months after surgery. There was no significant difference between the two groups in the maximum urinary flow rate (Q max), postvoid residual volume (PVR), international prostate symptom score (IPSS), or quality-of-life score (QOL score) at 1 month after surgery (p = 0.56, p = 0.346, p = 0.536 and p = 0.145, respectively). However, after 12 months, patients from the HoLEP group demonstrated better scores in Qmax, PVR, IPSS, and QOL than those from the TURP group (p = 0.037, p = 0.003, p < 0.001 and p = 0.019, respectively). The two groups had comparable operation time (p = 0.105), catheterization time (p = 0.173), and length of hospital stay (p = 0.395), but were statistically different in the weight of resected prostate tissue (p < 0.001), bladder irrigation time (p < 0.001), hemoglobin levels (p = 0.011), and blood sodium levels (p = 0.002) after surgery. Compared to TURP, HoLEP was safer and had better long-term efficacy as assessed by multiple quantitative measures. Therefore, HoLEP may present a better option in the treatment of BPH.

  3. Transurethral Bipolar Enucleation of the Prostate Is an Effective Treatment Option for Men With Urinary Retention.

    Science.gov (United States)

    Tracey, James M; Warner, Jonathan N

    2016-01-01

    To evaluate outcomes of transurethral bipolar enucleation (TuBE) of the prostate in patients with refractory lower urinary tract symptoms. A retrospective analysis was performed on patients who underwent TuBE from July 2014 to March 2015. Perioperative factors evaluated included International Prostate Symptom Score, Sexual Health Inventory for Men score, prostate-specific antigen, postvoid residual volume, transrectal ultrasound volume measurement, estimated blood loss, operative time, pathologic weight, and complications. Postoperative evaluation was performed at 6 weeks and 3 months. Forty-nine patients were identified. Mean age was 67 years and mean follow-up was 4.4 months. Twenty-eight patients (57%) were in retention. Preoperative, 6-week, and 3-month mean postvoid residual volumes were 278 mL, 66 mL, and 87 mL (P < .01); mean International Prostate Symptom Scores were 22, 9, and 8 (P  <  .01); mean quality of life scores were 5.0, 1.9, and 1.9; and Sexual Health Inventory for Men scores were 7.1, 8.4, and 7.0 (P = .35), respectively. Twenty-eight patients (57%) were able to have erections preoperatively and were still able to postoperatively. All (100%) of the patients in retention were able to void postoperatively. Mean operative time was 93 minutes, estimated blood loss was 49 mL, and pathologic weight was 18 g. Urinary tract infection occurred in 3 patients (6%), urethral stricture in 1 (2%), and bladder neck contracture in 2 (4%). Mean prostate-specific antigen decreased from 3.2 ng/dL to 0.9 ng/dL at 3 months (P < .01). TuBE is an effective operation for refractory urinary tract symptoms including those who are in urinary retention. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. Holmium laser enucleation versus transurethral resection of the prostate: a comparison of clinical results.

    Science.gov (United States)

    Basić, Dragoslav; Stanković, Jablan; Potić, Milan; Ignjatović, Ivan; Stojković, Ivica

    2013-01-01

    Holmium laser enucleation of the prostate (HoLEP) represents a great potential alternative technique to standard transurethral resection of the prostate (TURP). We present 12-month follow up results of a randomized clinical trial, comparing HoLEP with TURP. A total of 40 patients with BPH and prostate volume < 50 g, have been randomized for HoLEP (n = 20) or TURP (n = 20). Urinary tract ultrasound with postvoid residual urine (PVR), International Prostate Symptom Score (IPSS) and Single Question Quality of Life (QoL) Score were evaluated preoperatively and during the follow-up period at 1, 3, 6, and 12 mo postoperatively. Intra- and perioperative data as well as early and late complications were assessed. Operative time was longer in the HoLEP group (p < 0.001); catheterisation time (p < 0.05) and hospital stay (p < 0.05) shorter. Hemoglobin levels drop (p < 0.001) was higher in the TURp group. Early and late postoperative complications were more frequent in the TURp group (p < 0.001). Follow-up data favored the HoLEP group, both regarding IPSS at 6-month (p < 0.05) and 12-month (p < 0.01), and single question QoL score, at 6-month (p < 0.01) and 12-month (p < 0.05). PVR was lower in the HoLEP group at 6-month (p < 0.01). HoLEP demonstrates superiority to TURp in regards to perioperative parameters and follow-up data and has a great potential to become the new gold standard in the surgical treatment of BPH.

  5. Early outcome of transurethral enucleation and resection of the prostate versus transurethral resection of the prostate.

    Science.gov (United States)

    Palaniappan, Sundaram; Kuo, Tricia Li Chuen; Cheng, Christopher Wai Sam; Foo, Keong Tatt

    2016-12-01

    Recurrent prostate adenoma is a long-term complication following transurethral resection of the prostate (TURP). Transurethral enucleation and resection of the prostate (TUERP) is more appealing, since the nodular adenoma can be completely removed through endoscopy. TUERP is also hypothesised to result in a lower frequency of recurrent adenoma. This study aimed to compare the early outcomes of TUERP and TURP, and assess the feasibility and safety of TUERP. We compared the outcome of 81 patients who underwent TUERP with that of 85 patients who underwent TURP. International prostate symptom score, quality of life score, prostate volume, degree of intravesical prostatic protrusion, maximum flow rate, post-void residual volume and prostate-specific antigen (PSA) level were obtained pre- and postoperatively. Complications (e.g. transfusion rate, incontinence, infection and urethral stricture) were analysed. Operative time was significantly longer in the TUERP group compared to the TURP group (85.3 minutes vs. 51.6 minutes). After TUERP, the maximum flow rate was significantly higher (21.1 mL/s vs. 17.1 mL/s) and PSA level was significantly lower (1.2 ng/mL vs. 1.9 ng/mL) than after TURP. The rates of infection, transfusion and urethral stricture were similar for both groups, but the TUERP group had a higher rate of temporary incontinence (13.6% vs. 4.7%). The lower PSA level and better maximum flow rate achieved following TUERP suggest that prostate adenoma removal was more complete with TUERP. Long-term follow-up is required to establish whether TUERP results in fewer resections for recurrent adenoma.

  6. Holmium Laser enucleation of the prostate (HoLEP) versus Transurethral Resection of the Prostate (TURP).

    Science.gov (United States)

    Barboza, Luís Eduardo Durães; Malafaia, Osvaldo; Slongo, Luiz Edison; Meyer, Fernando; Nassif, Paulo Afonso Nunes; Tabushi, Fernando Issamu; Wendler, Eduardo; Beraldi, Rafael Alexandre

    2015-06-01

    to evaluate the effectiveness and applicability of Holmium laser enucleation of the prostate (HoLEP) - in the treatment of benign prostatic hyperplasia (BPH) - in comparison to transurethral resection of the prostate (TURP). patients with symptomatic prostatic hyperplasia and candidates for surgical treatment were selected. Both procedures were explained and they had choosen HoLEP or TURP. At the hospital were collected: age, date of birth, international prostate symptom score, urinary peak flow rate, prostate volume, post-voiding residual urine, globular volume and serum PSA. At the procedure operating time, morcellating time (HoLEP), bladder mucosal injury and intercurrences were collected. At the first postoperative day, globular volume and sodium. Besides that were observed the catheter indwelling time and hospital stay and after 90 days, urinary peak flow rate and international prostate symptom score. Statistical analisys have been done partially by Sinpe(r) and also by a professional team. twenty patients in HoLEP group and 21 at TURP were operated. Baseline urinary peak flow rate was 8 ml/s in both groups and preoperative international prostate symptom score was 22 in HoLEP and 20 in TURP, very similar. Operative time was 85 minutes in HoLEP and 60 in TURP, p<0.05. Hospital stay was 47 hours for HoLEP and 48 hours to TURP, p<0.05. At 90 day the urinary peak flow rate was raised to 21.5 ml/s in HoLEP group and to 20 ml/s in TURP and the median of international prostate symptom score had been reduced to score 3 in both groups. HoLEP is a feasible technique and is as effective as TURP on symptomatic prostatic hyperplasia surgical treatment.

  7. Factors involved in the occurrence of bleeding complications after enucleation for cornea donation.

    Science.gov (United States)

    van Wijk, Marja J; Nijenhuis, Matthijs V; Dorrepaal, Caroline A; Bokhorst, Arlinke G

    2009-10-01

    To identify risk factors for the occurrence of bleeding complications after enucleation for cornea donation, in order to develop preventive activities to reduce the occurrence of bleeding complications and especially the development of ocular hematomas. From all Dutch cornea donors deceased in the year 2006, donor characteristics, retrieval characteristics, and bleeding complication data were collected. First, univariate relations between donor and retrieval factors and bleeding complications were determined. Then, multivariate logistic regression analysis was used to identify factors significantly associated with bleeding complications. In 114 of the 1173 cornea donors (9.7%), bleeding complications occurred, with ocular hematomas developing in 39 (3.3% of all cornea donors). Donor factors significantly associated with bleeding complications were age [odds ratio (OR) 0.96 (95% CI 0.94-0.97)], weight [OR 1.03 (1.02-1.04)], heart failure [OR 2.10 (1.12-3.94)], thrombocyte aggregation inhibitor use [OR 1.64 (1.02-2.64)], and chronic alcoholic liver disease [OR 2.85 (1.11-7.31)]. The most significant factor associated with bleeding complications was the retrieval sequence. If cornea donation was followed by any other tissue retrieval, the risk of bleeding complications was strongly increased. : This study shows that the tissue retrieval sequence in multitissue donors is the most important factor associated with the occurrence of bleeding complications. The risk of bleeding and ocular hematoma is lower if cornea donation is performed after all other retrievals. However, if the tissue retrieval sequence is altered, the effect of prolonged postmortem time on corneal quality must be taken into account.

  8. An automation-assisted generic approach for biological sample preparation and LC-MS/MS method validation.

    Science.gov (United States)

    Zhang, Jie; Wei, Shimin; Ayres, David W; Smith, Harold T; Tse, Francis L S

    2011-09-01

    Although it is well known that automation can provide significant improvement in the efficiency of biological sample preparation in quantitative LC-MS/MS analysis, it has not been widely implemented in bioanalytical laboratories throughout the industry. This can be attributed to the lack of a sound strategy and practical procedures in working with robotic liquid-handling systems. Several comprehensive automation assisted procedures for biological sample preparation and method validation were developed and qualified using two types of Hamilton Microlab liquid-handling robots. The procedures developed were generic, user-friendly and covered the majority of steps involved in routine sample preparation and method validation. Generic automation procedures were established as a practical approach to widely implement automation into the routine bioanalysis of samples in support of drug-development programs.

  9. Tunable microwave-assisted method for the solvent-free and catalyst-free peracetylation of natural products

    Directory of Open Access Journals (Sweden)

    Manuela Oliverio

    2016-10-01

    Full Text Available Background: The peracetylation is a simple chemical modification that can be used to enhance the bioavailability of hydrophilic products and to obtain safe and stable pro-drugs.Results: A totally green, solvent-free and catalyst-free microwave (MW-assisted method for peracetylation of natural products such as oleuropein, alpha-hederin, quercetin and rutin is presented. By simply tuning the MW heating program, polyols with chemical diverse –OH groups or thermolabile functionalities can be peracetylated to improve the biological activity without degradation of the natural starting molecules. An evaluation of the process greenness was performed.Conclusion: The method is potentially universally applicable for green acetylation of hydrophilic biological molecules, potentially easily scalable for industrial applications, including pharmaceutical, cosmetic and food industry.

  10. HPLC and chemometric assisted spectrophotometric methods for simultaneous determination of diprophylline, phenobarbitone and papaverine hydrochloride.

    Science.gov (United States)

    El-Gindy, Alaa

    2005-09-01

    Three methods are developed for the simultaneous determination of diprophylline (DP), phenobarbitone (PH) and papaverine hydrochloride (PP). The chromatographic method depends on a high performance liquid chromatographic (HPLC) separation on a reversed-phase C18 column with a mobile phase consisting of 0.02 M potassium dihydrogen phosphate, pH 3.5--acetonitrile (55:45 v/v). Quantitation was achieved with UV detection at 210 nm based on peak area. The other two chemometric methods applied were principal component regression (PCR) and partial least squares (PLS-1). These approaches were successfully applied to quantify the three drugs in the mixture using the information included in the UV absorption spectra of appropriate solutions in the range 215-245 nm with the intervals Delta lambda = 0.2 nm. The calibration PCR and PLS-1 models were evaluated by internal validation (prediction of compounds in its own designed training set of calibration), by cross-validation (obtaining statistical parameters that show the efficiency for a calibration fit model) and by external validation over laboratory-prepared mixtures and pharmaceutical preparations. The PCR and PLS-1 methods require neither any separation step, nor any priori graphical treatment of the overlapping spectra of the three drugs in a mixture. The results of PCR and PLS-1 methods were compared with HPLC method obtained in pharmaceutical formulation and a good agreement was found.

  11. extrap: Software to assist the selection of extrapolation methods for moving-boat ADCP streamflow measurements

    Science.gov (United States)

    Mueller, David S.

    2013-04-01

    Selection of the appropriate extrapolation methods for computing the discharge in the unmeasured top and bottom parts of a moving-boat acoustic Doppler current profiler (ADCP) streamflow measurement is critical to the total discharge computation. The software tool, extrap, combines normalized velocity profiles from the entire cross section and multiple transects to determine a mean profile for the measurement. The use of an exponent derived from normalized data from the entire cross section is shown to be valid for application of the power velocity distribution law in the computation of the unmeasured discharge in a cross section. Selected statistics are combined with empirically derived criteria to automatically select the appropriate extrapolation methods. A graphical user interface (GUI) provides the user tools to visually evaluate the automatically selected extrapolation methods and manually change them, as necessary. The sensitivity of the total discharge to available extrapolation methods is presented in the GUI. Use of extrap by field hydrographers has demonstrated that extrap is a more accurate and efficient method of determining the appropriate extrapolation methods compared with tools currently (2012) provided in the ADCP manufacturers' software.

  12. Multicentre prospective evaluation of the learning curve of holmium laser enucleation of the prostate (HoLEP).

    Science.gov (United States)

    Robert, Grégoire; Cornu, Jean-Nicolas; Fourmarier, Marc; Saussine, Christian; Descazeaud, Aurélien; Azzouzi, Abdel-Rahmène; Vicaut, Eric; Lukacs, Bertrand

    2016-03-01

    To describe the step-by-step learning curve of the holmium laser enucleation of the prostate (HoLEP) surgical technique. A prospective, multicentre observational study was conducted, involving surgeons experienced in transurethral resection of the prostate and open prostatectomy but never having performed HoLEP. The main judgment criterion was the ability of the surgeon to perform four consecutive successful procedures, defined by the following: complete enucleation and morcellation within transurethral resection of the prostate (TURP), with acceptable stress, and with acceptable difficulty (evaluated by Likert scales). Each surgeon included 20 consecutive cases. Of nine centres, three abandoned HoLEP before the end of the study due to complications, and one was excluded for treating patients off protocol. Only one centre achieved the main judgment criterion of four consecutive successful HoLEP procedures. Overall, the procedures were successfully performed in 43.6% of cases. Reasons for unsuccessful procedures were mainly operative time >90 min (n = 51), followed by conversion to TURP (n = 14), incomplete morcellation (n = 8), significant stress (n = 9), or difficulty (n = 14) during HoLEP. Ignoring operating time, 64% of procedures were successful and four out of five centres did four consecutive successful cases. Of the five centres that completed the study, four chose to continue HoLEP. Even in a prospective training structure, HoLEP has a steep learning curve exceeding 20 cases, with almost half of our centres choosing to abandon or not to continue with the technique. Operating time and difficulty of the enucleation seem the most important problems for a beginner. A more intensely mentored and structured mentorship programme might allow safer adoption of the procedure. © 2015 The Authors BJU International © 2015 BJU International Published by John Wiley & Sons Ltd.

  13. Evaluation of the Learning Curve for Transurethral Plasmakinetic Enucleation and Resection of Prostate Using a Mentor-based Approach.

    Science.gov (United States)

    Feng, Lang; Song, Jian; Zhang, Daoxin; Tian, Ye

    2017-01-01

    To analyze the mentor-based learning curve of one single surgeon with transurethral plasmakinetic enucleation and resection of prostate (PKERP) prospectively. Ninety consecutive PKERP operations performed by one resident under the supervision of an experienced endourologist were studied. Operations were analyzed in cohorts of 10 cases to determine when a plateau was reached for the variables such as operation efficiency, enucleation efficiency and frequency of mentor advice (FMA). Patient demographic variables, perioperative data, complications and 12-month follow-up data were analyzed and compared with the results of a senior urologist. The mean operative efficiency and enucleation efficiency increased from a mean of 0.49±0.09g/min and 1.11±0.28g/min for the first 10 procedures to a mean of 0.63±0.08g/min and 1.62±0.36g/min for case numbers 31-40 (p=0.003 and p=0.002). The mean value of FMA decreased from a mean of 6.7±1.5 for the first 10 procedures to a mean of 2.8±1.2 for case numbers 31-40 (penucleation efficiency equivalent to those of the senior resident after 40 cases. There was significant improvement in 3, 6 and 12 month's parameter compared with preoperative values (p<0.001). PKERP can be performed safely and efficiently even during the initial learning curve of the surgeon when closely mentored. Further well-designed trials with several surgeons are needed to confirm the results. Copyright® by the International Brazilian Journal of Urology.

  14. Holmium Laser Enucleation of the Prostate versus Laparoscopic Transcapsular Prostatectomy: Perioperative Results and Three-Month Follow-Up.

    Science.gov (United States)

    Baldini, Arnaud; Fassi-Fehri, Hakim; Duarte, Ricardo C; Crouzet, Sebastien; Ecochard, René; Abid, Nadia; Martin, Xavier; Badet, Lionel; Colombel, Marc

    2017-07-01

    Symptomatic benign prostatic hypertrophy greater than 70 cc used to be treated by invasive procedures. Holmium laser enucleation of prostate (HoLEP) and laparoscopic transcapsular prostatectomy (LTP) are two techniques whose efficacy has been demonstrated compared to standard onesmore invasive standard procedures. The objective was to evaluate and compare perioperative results from these two techniques for the treatment of benign prostatic hypertrophy greater than 70 cc. This was a non-randomized retrospective study comparing the HoLEP technique with LTP. From January 2012 to January 2015, 39 patients had HoLEP and 28 had LTP. Perioperative outcomes, complications, and functional results at 3 months were compared. A chi-2 squared test and Student's t test were used for statistical analysis. In multivariate analysis, there was a statistically significant difference in favor of HoLEP for the duration of catheterization (1.9 vs. 3.7 days; p = 0.004) and the average length of stay (2.8 vs. 4.0 days, p = 0.010). There was a trend towards a greater decrease in postoperative hemoglobin levels in LTP (138 vs. 218 g/l; p = 0.082), which was statistically significant in univariate analysis (p = 0.033). Other endpoints were not significant, particularly the enucleated prostate volume compared to the total prostate volume (61.8 vs. 68.4%; p = 0.319) and postoperative complications. There was no increased morbidity for LTP compared to the HoLEP technique. However, the HoLEP technique appeared to be a less invasive technique, reducing the duration of catheterization, blood loss, and the average length of stay while maintaining good efficacy for the enucleated prostate volume.

  15. New experimental setup for boron isotopes separation by laser assisted retardation of condensation method

    Energy Technology Data Exchange (ETDEWEB)

    Lyakhov, K. A.; Lee, H. J. [Institute for Nuclear Science and Technology, Jeju (Korea, Republic of)

    2013-05-15

    Proposed static model corresponds to the two-cycle iterative scheme for boron isotopes separation. This rather simple model helps to understand combined action of all important parameters and relations between them on boron isotopes separation by SILARC method. These parameters include carrier gas choice, molar fraction at what BCl{sub 3} gas is dissolved in carrier gas, laser intensity, providing minimum of energy expenses and the largest output, optimal gas pressure and temperature in backing and downstream chambers, optimal irradiation cell and skimmer chamber dimensions, optimal nozzle throughput. It was suggested a method for finding optimal values of these parameters based on global minimum or energy spent on production of unit of isotope, which is analog of Separative Work Unit (SWU). It was shown that the most optimal carrier gas, corresponding to this minimum, among other considered-N{sub 2},NO{sub 2},SF{sub 6}, is Ar. Restriction on the maximal irradiation chamber length, caused by dimerization rate, is also considered. Two types of industrial scale irradiation cells are compared. The first one has the only large throughput slit nozzle, while the second one has numerous small nozzles arranged in parallel arrays for better overlap with laser beam. It is shown that the last one outperforms the former one significantly. We calculated that our experimental setup, provided argon is used as a carrier gas, can yield ∼50mg/hr of boron-10. It is interesting to compare this production rate with traditional method of industrial boron isotopes separation by low temperature distillation, Ref. In this method production rate is practically the same, but energy expenses per separated isotope are substantially smaller: ∼1 eV/atom instead of ∼10{sup 3} eV/atom as for SILARC method. The largest fraction of energy expenses in our SILARC-based method comes from compressor operation.

  16. Thermoluminescence study of Cu and Ag doped lithium tetraborate samples synthesized by water/solution assisted method

    Energy Technology Data Exchange (ETDEWEB)

    Thiyagarajan, S.; Kumar, S.; Vallejo, M.; Sosa, M. [Universidad de Guanajuato, Departamento de Ingenieria Fisica, 37150 Leon, Guanajuato (Mexico); Velusamy, J., E-mail: thiya93@gmail.com [Centro de Investigaciones en Optica, Apdo. Postal 1-948, Leon, Guanajuato (Mexico)

    2016-10-15

    In this paper lithium tetraborate (Li{sub 2}B{sub 4}O{sub 7}) was produced by water/solution assisted synthesis method. Transition metals, such as Cu and Ag were used to dope Li{sub 2}B{sub 4}O{sub 7} in order to enhance its thermoluminescent properties. The heating temperature parameters for synthesis were 750 degrees Celsius for 2 hours and 150 degrees Celsius for another 2 hours. The samples produced by water assisted method were doped at different doping percentage (0.08, 0.12, 0.5, 0.1 and 1%) of Cu and Ag. Pellets of samples were prepared and there were irradiated with different doses (58, 100, 500 and 945 mGy) by using and X-ray source. The characteristics of undoped and doped Li{sub 2}B-4O{sub 7} were determined by X-ray diffraction (XRD), scanning electron microscopy (Sem), photoluminescence and ultraviolet-visible spectroscopy. The chemical composition and their morphologies of the obtained Li{sub 2}B{sub 4}O{sub 7} and Li{sub 2}B{sub 4}O{sub 7}:Cu, Ag was confirmed by XRD and Sem results. The most intense peak of the XRD pattern of the lithium tetraborate sample was determined by comparing to the reference data and was found to have a tetragonal structure. The thermoluminescent glow curves of the pellets exposed to different doses exhibited a clear response to X-ray irradiation. Especially Li{sub 2}B{sub 4}O{sub 7}:Cu presented a good glow curve in all kind of doses. The experimental results showed that this could have good potential applications in radiation dosimetry. The order of kinetics (b), frequency factor (s) and activation energy (E) or the trapping parameters were calculated using peak shape method. (Author)

  17. Determination of residues of sulfonylurea herbicides in soil by using microwave-assisted extraction and high performance liquid chromatographic method

    Directory of Open Access Journals (Sweden)

    Grahovac Nada L.

    2017-01-01

    Full Text Available A modified method for the analysis of nicosulfuron, rimsulfuron and prosulfuron was developed and validated by using microwave-assisted extraction (MAE and ultra-performance liquid chromatography with diode array detection in the ultraviolet region (HPLC-UV-DAD. The most important experimental parameters of extraction procedure and HPLC-UV-DAD technique were optimised in respect to those sulfonylurea herbicides. High recoveries of the microwave-assisted extraction were obtained by using a dichloromethane–acetonitrile mixture (2:1 volume ratio acidified with acetic acid (0.8 vol.% with the addition of urea. The mean recoveries at three spiking levels ranged from 97.47 to 98.76% for nicosulfuron, 97.88 to 99.17% for rimsulfuron and from 97.91 to 99.83% for prosulfuron. The limits of detection of nicosulfuron, rimsulfuron and prosulfuron were 0.95, 0.91 and 0.89 μg kg–1, respectively. The accuracy of the developed method was confirmed by HPLC coupled with tandem mass spectrometry parallel analyses. The developed method was used to investigate the dissipation dynamics of sulfonylurea herbicides in the real field trials in Vojvodina Province, Serbia. The obtained half-lives were 0.05, 0.23 and 0.15 days for recommended dose application of nicosulfuron, rimsulfuron and prosulfuron, respectively. Low residues and short half-life in soil suggested that the risk to sensitive rotational crops after application of those sulfonylurea herbicides is low when they are used in the appropriate dosages. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31072

  18. Comparison of fluid absorption during transurethral resection of prostate and Holmium-Yag laser enucleation of benign adenoma of prostate using breath ethanol concentration

    Directory of Open Access Journals (Sweden)

    Shivadeo Bapat

    2007-01-01

    Full Text Available Objective: We conducted a study to detect, quantify and compare irrigation fluid absorption in transurethral resection of the prostate (TURP and Holmium laser enucleation of the prostate (HoLEP, using BEC. Materials and Methods: The study included 50 patients of lower urinary tract symptoms, secondary to benign enlargement of prostate. The patients were nonrandomly allocated to undergo TURP and HoLEP. Twenty-six patients underwent TURP and the remaining 24 underwent HoLEP. Sterile water tagged with 1% ethanol w/v was used for irrigation. Absorption was detected and quantified every 10min by BEC levels. Data was analyzed using standard nomograms. Results: In HoLEP, 14/24 had no fluid absorption. The remaining 10/24 showed fluid absorption ranging from 95 ml to 300 ml. In TURP, all had fluid absorption ranging from 250-980 ml. Three TURP patients developed overt symptoms, while none did in the HoLEP group. Conclusions: Fluid absorption observed in our study in the HoLEP group was lower than in the TURP group.

  19. Efficacy and safety of holmium laser enucleation of the prostate for extremely large prostatic adenoma in patients with benign prostatic hyperplasia

    Science.gov (United States)

    Kim, Myong; Piao, Songzhe; Lee, Hahn-Ey; Kim, Sung Han

    2015-01-01

    Purpose To evaluate the efficacy and safety of holmium laser enucleation of the prostate (HoLEP) for extremely large prostates. Materials and Methods Patients undergoing HoLEP between July 2008 and December 2013 from the Seoul National University Hospital Benign Prostatic Hyperplasia Database Registry were retrospectively analyzed. The patients were divided into three groups according to their total prostate volume (TPV): group A (TPVenucleation and morcellation times were longer in group C (penucleation efficacy was higher in this group (p<0.001, R2=0.399). Moreover, the mean postoperative catheterization and hospitalization periods were significantly longer in group C (p=0.004 and p=0.011, respectively). However, there were no significant differences between the groups in any other postoperative events, including recatheterization, reoperation, urinary tract infection, clot retention, and bladder neck contracture (p range, 0.516-0.913). One patient in group C experienced recurrence of the urethral stricture. Conclusions HoLEP in patients with an extremely large prostate can be performed efficiently and safely. PMID:25763126

  20. Thulium laser versus holmium laser transurethral enucleation of the prostate: 18-month follow-up data of a single center.

    Science.gov (United States)

    Zhang, Fengbo; Shao, Qiang; Herrmann, Thomas R W; Tian, Ye; Zhang, Yuhai

    2012-04-01

    To compare the clinical outcomes between thulium laser transurethral enucleation of the prostate (ThuLEP) (70 W) and holmium laser transurethral enucleation of the prostate (HoLEP) (90 W) in a prospective randomized trial with 18 months of follow-up. Both ThuLEP and HoLEP effectively relieve the obstructive symptoms due to benign prostatic hyperplasia (BPH). A total of 133 consecutive patients with BPH were randomized to either ThuLEP (n = 71) or HoLEP (n = 62). An energy setting of 70 W and 90 W was used for the thulium and holmium laser in the enucleation procedure, respectively. The mushroom technique was used to fragment the enucleated lobes with the resection loop. The preoperative and postoperative parameters were compared. ThuLEP required a longer operation time (72.4 vs 61.5 minutes, P = .034) but resulted in less blood loss than HoLEP (130.0 vs 166.6 mL, P = .045). The catheterization time was comparable. At 18 months, the lower urinary tract symptom indexes were improved significantly in both groups compared with the baseline values. The International Prostate Symptom Score decreased to 5.2 in the ThuLEP group and 6.2 in the HoLEP group. The quality of life score and peak urinary flow rate were similar between the 2 groups (1.3 vs 1.2 and 23.4 vs 24.2 mL/s) and the postvoid residual urine volume decreased by 82.50% and 81.73% in the ThuLEP and HoLEP groups, respectively. The mean prostate-specific antigen decrease after HoLEP and ThuLEP was 30.43% and 43.36%, respectively. No urethral or bladder neck stricture were found in either group. Both ThuLEP (70 W) and HoLEP (90 W) relieve lower urinary tract symptoms equally with high efficacy and safety. ThuLEP was statistically superior to HoLEP in blood loss and inferior to HoLEP in operation time, although the differences were clinically negligible. The mushroom technique could be adequate, without an additional mechanical tissue morcellator. Copyright © 2012 Elsevier Inc. All rights reserved.

  1. A unique case of facial burn superinfected with Dermatobia Hominis larvae resulting in a bilateral enucleation of the eyes.

    Science.gov (United States)

    Pinos, Victor Hugo; Ortiz-Prado, Esteban; Bermeo, Carlos; León, Juan; Armijos, Luciana; Almeida, Estibaliz

    2014-10-01

    We present a case of a female Ecuadorian patient who presented a deep facial burn injury complicated with a severe infestation of Dermatobia Hominis larvae. The burn injury was complicated by severe myiasis attributable to the poor management of the wound received at home, using tropical plants, which caused a secondary infection and severe necrosis of the tissue involving the forehead, cheeks, chin, scalp, nose, mouth and the eyes resulting in a bilateral enucleation and long inpatient hospital care. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  2. Electric Field-Assisted Matrix Coating Method Enhances the Detection of Small Molecule Metabolites for Mass Spectrometry Imaging.

    Science.gov (United States)

    Guo, Shuai; Wang, Yanmin; Zhou, Dan; Li, Zhili

    2015-06-16

    Small molecule metabolites (SMMs, typically matrix and/or low sensitivity. Herein, we develop an electric field-assisted scanning-spraying (EFASS) matrix coating system to deposit matrix on tissue with crystal sizes of matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) in negative ion mode. A comparison with other matrix deposition methods (i.e., airbrush and sublimation) using common matrixes (i.e., N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC), 9-aminoacridine (9-AA), 2,5-dihydroxybenoic acid (DHB)) indicated that the EFASS system could effectively enhance detection sensitivity and the number of tissue SMMs detected. MSI of five gastric cancer tissues coated with NEDC by the EFASS system demonstrated that significantly increased levels of fatty acids (i.e., palmitic acid and oleic acid) and nucleosides monophosphate (i.e., uridine monophosphate, adenosine monophosphate, and guanosine monophosphate) and significantly decreased levels of nucleosides (i.e., inosine, guanosine, and uridine) and N-acetylneuraminic acid were observed in cancerous areas.

  3. Participant Assisted Data Collection Methods in the California Healthy Homes Indoor Air Quality Study of 2011-13

    Energy Technology Data Exchange (ETDEWEB)

    Mullen, Nasim A. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Li, Jina [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Singer, Brett C. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2013-08-01

    From November 2011 to March 2013, air quality was measured over 6-day periods in 324 residences across California using a mail-out strategy. All interactions with study participants, from recruitment, to data collection, to communication of results, were conducted with remote communication methods including conventional mail, electronic mail, telephone and text messaging. Potential participants were reached primarily by sharing study information with community groups and organizations that directed interested individuals to complete an online screening survey. Pollutant concentrations were measured with sampling equipment that was mailed to participants' homes with deployment instructions. Residence and household characteristics and activity data were collected via two phone surveys and an activity log. A comparison of responses to survey questions completed online versus over the phone indicated that a substantial fraction of participants (roughly 20%) required a researcher's assistance to respond to basic questions about appliance characteristics. Using the printed instructions and telephone assistance from researchers, roughly 90% of participants successfully deployed and returned sampling materials accurately and on schedule. The mail-out strategy employed in this study was found to be a cost-effective means for collecting residential air quality data.

  4. Urea free and more efficient sample preparation method for mass spectrometry based protein identification via combining the formic acid-assisted chemical cleavage and trypsin digestion.

    Science.gov (United States)

    Wu, Shuaibin; Yang, Kaiguang; Liang, Zhen; Zhang, Lihua; Zhang, Yukui

    2011-10-30

    A formic acid (FA)-assisted sample preparation method was presented for protein identification via mass spectrometry (MS). Detailedly, an aqueous solution containing 2% FA and dithiothreitol was selected to perform protein denaturation, aspartic acid (D) sites cleavage and disulfide linkages reduction simultaneously at 108°C for 2h. Subsequently, FA wiped off via vacuum concentration. Finally, iodoacetamide (IAA) alkylation and trypsin digestion could be performed ordinally. A series of model proteins (BSA, β-lactoglobulin and apo-Transferrin) were treated respectively using such method, followed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The identified peptide number was increased by ∼ 80% in comparison with the conventional urea-assisted sample preparation method. Moreover, BSA identification was achieved efficiently down to femtomole (25 ± 0 sequence coverage and 16 ± 1 peptides) via such method. In contrast, there were not peptides identified confidently via the urea-assisted method before desalination via the C18 zip tip. The absence of urea in this sample preparation method was an advantage for the more favorable digestion and MALDI-TOF MS analysis. The performances of two methods for the real sample (rat liver proteome) were also compared, followed by a nanoflow reversed-phase liquid chromatography with electrospray ionization tandem mass spectrometry system analysis. As a result, 1335 ± 43 peptides were identified confidently (false discovery rate method, corresponding to 295 ± 12 proteins (of top match=1 and requiring 2 unique peptides at least). In contrast, there were only 1107 ± 16 peptides (corresponding to 231 ± 10 proteins) obtained from the conventional urea-assisted method. It was serving as a more efficient protein sample preparation method for researching specific proteomes better, and providing assistance to develop other proteomics analysis methods, such as, peptide

  5. Synthesis of nanostructured and microstructured ZnO and Zn(OH)2 on activated carbon cloth by hydrothermal and microwave-assisted chemical bath deposition methods

    Science.gov (United States)

    Mosayebi, Elham; Azizian, Saeid; Hajian, Ali

    2015-05-01

    Nanostructured and microstructured ZnO and Zn(OH)2 loaded on activated carbon cloth were synthesized by microwave-assisted chemical bath deposition and hydrothermal methods. By hydrothermal method the deposited sample on carbon fiber is pure ZnO with dandelion-like nanostructures. By microwave-assisted chemical bath method the structure and composition of deposited sample depends on solution pH. At pH = 9.8 the deposited sample on carbon fiber is pure ZnO with flower-like microstructure; but at pH = 10.8 the sample is a mixture of ZnO and Zn(OH)2 with flower-like and rhombic microstructures, respectively. The mechanism of crystal grow by microwave-assisted chemical bath method was investigated by SEM method at both pH.

  6. Adaptive Finite Element Method Assisted by Stochastic Simulation of Chemical Systems

    KAUST Repository

    Cotter, Simon L.

    2013-01-01

    Stochastic models of chemical systems are often analyzed by solving the corresponding Fokker-Planck equation, which is a drift-diffusion partial differential equation for the probability distribution function. Efficient numerical solution of the Fokker-Planck equation requires adaptive mesh refinements. In this paper, we present a mesh refinement approach which makes use of a stochastic simulation of the underlying chemical system. By observing the stochastic trajectory for a relatively short amount of time, the areas of the state space with nonnegligible probability density are identified. By refining the finite element mesh in these areas, and coarsening elsewhere, a suitable mesh is constructed and used for the computation of the stationary probability density. Numerical examples demonstrate that the presented method is competitive with existing a posteriori methods. © 2013 Society for Industrial and Applied Mathematics.

  7. System and method for laser assisted sample transfer to solution for chemical analysis

    Science.gov (United States)

    Van Berkel, Gary J; Kertesz, Vilmos

    2014-01-28

    A system and method for laser desorption of an analyte from a specimen and capturing of the analyte in a suspended solvent to form a testing solution are described. The method can include providing a specimen supported by a desorption region of a specimen stage and desorbing an analyte from a target site of the specimen with a laser beam centered at a radiation wavelength (.lamda.). The desorption region is transparent to the radiation wavelength (.lamda.) and the sampling probe and a laser source emitting the laser beam are on opposite sides of a primary surface of the specimen stage. The system can also be arranged where the laser source and the sampling probe are on the same side of a primary surface of the specimen stage. The testing solution can then be analyzed using an analytical instrument or undergo further processing.

  8. Macro cell assisted cell discovery method for 5G mobile networks

    DEFF Research Database (Denmark)

    Marcano, Andrea; Christiansen, Henrik Lehrmann

    2016-01-01

    , and requires a new system design. The aspects concerning the impact of using mmWave frequencies on the medium access (MAC) layer are one of the topics that need to be further analyzed. In this article we focus on the cell discovery process of the MAC laywe for mmWave communications. A new approach assuming...... a joint search of the user equipment (UE) between the mmWave small cell (SC) and the macro cell (MC) is proposed. The performance of this method is analyzed and compared with existing methods. The results show that using the MC as aid during the search process can allow for up to 99% improvement in terms...

  9. Prosthetic Rehabilitation Following Socket Reconstruction with Blair-Brown Graft and Conformer Therapy for Management of Severe Post-Enucleation Socket Syndrome--A Clinical Report.

    Science.gov (United States)

    Aggarwal, Himanshi; Singh, Saumyendra V; Kumar, Pradeep; Kumar Singh, Arun

    2015-06-01

    One of the most common tumors of the eye diagnosed in childhood is retinoblastoma, which mandates enucleation with adjunctive chemotherapy and radiotherapy to save the patient's life. The most common late enucleation complication is post-enucleation socket syndrome (PESS), which poses a management dilemma for the prosthodontist and surgeon, along with being a major esthetic concern for the patient. The reconstruction of such sockets is complex. The purpose of this clinical report is to describe the rehabilitation of such a pediatric patient with severe PESS. The patient was successfully rehabilitated by presurgical conformer therapy, socket reconstruction surgery with non-meshed intermediate split thickness skin graft (STSG)/Blair-Brown graft, and postsurgical conformer stent. This was followed by fabrication of a custom ocular prosthesis, to achieve favorable functional, physical, and psychological effects. © 2014 by the American College of Prosthodontists.

  10. Bone dissemination of prostate cancer after holmium laser enucleation of the prostate: a case report and a review of the literature.

    Science.gov (United States)

    Koguchi, Dai; Nishi, Morihiro; Satoh, Takefumi; Shitara, Toshiya; Matsumoto, Kazumasa; Fujita, Tetsuo; Yoshida, Kazunari; Iwamura, Masatsugu

    2014-02-01

    We report a case of dissemination of prostate cancer after holmium laser enucleation of the prostate in an 80-year-old patient. The patient presented at hospital because of nocturia. Transrectal ultrasound-guided biopsy was carried out because of high serum prostate-specific antigen (3.55 ng/mL), but it showed no malignancies. Benign prostate hyperplasia was diagnosed, and he was started on an α1-blocker. Although the urinary symptom improved with silodosin, acute urinary retention occurred 3 years after therapy began. Holmium laser enucleation of the prostate for relief of bladder outlet obstruction enabled discharge of urine. Pathological examination of the resected tissue found adenocarcinoma with a high Gleason score, 4 + 5. Serum alkaline phosphatase increased rapidly after holmium laser enucleation, and bone scintigraphy confirmed multiple bone metastases. Prostate cancer, T1bN0M1b, was diagnosed. © 2013 The Japanese Urological Association.

  11. Ultrasound assisted methods for enhanced extraction of phycobiliproteins from marine macro-algae, Gelidium pusillum (Rhodophyta).

    Science.gov (United States)

    Mittal, Rochak; Tavanandi, Hrishikesh A; Mantri, Vaibhav A; Raghavarao, K S M S

    2017-09-01

    Extraction of phycobiliproteins (R-phycoerythrin, R-PE and R-phycocyanin, R-PC) from macro-algae is difficult due to the presence of large polysaccharides (agar, cellulose etc.) present in the cell wall which offer major hindrance for cell disruption. The present study is aimed at developing most suitable methodology for the primary extraction of R-PE and R-PC from marine macro-algae, Gelidium pusillum(Stackhouse) Le Jolis. Such extraction of phycobiliproteins by using ultrasonication and other conventional methods such as maceration, maceration in presence of liquid nitrogen, homogenization, and freezing and thawing (alone and in combinations) is reported for the first time. Standardization of ultrasonication for different parameters such as ultrasonication amplitude (60, 90 and 120µm) and ultrasonication time (1, 2, 4, 6, 8 and 10mins) at different temperatures (30, 35 and 40°C) was carried out. Kinetic parameters were estimated for extraction of phycobiliproteins by ultrasonication based on second order mass transfer kinetics. Based on calorimetric measurements, power, ultrasound intensity and acoustic power density were estimated to be 41.97W, 14.81W/cm2 and 0.419W/cm3, respectively. Synergistic effect of ultrasonication was observed when employed in combination with other conventional primary extraction methods. Homogenization in combination with ultrasonication resulted in an enhancement in efficiency by 9.3% over homogenization alone. Similarly, maceration in combination with ultrasonication resulted in an enhancement in efficiency by 31% over maceration alone. Among all the methods employed, maceration in combination with ultrasonication resulted in the highest extraction efficiency of 77 and 93% for R-PE and R-PC, respectively followed by homogenization in combination with ultrasonication (69.6% for R-PE and 74.1% for R-PC). HPLC analysis was carried out in order to ensure that R-PE was present in the extract and remained intact even after processing

  12. Molecular beam epitaxy of GaAs nanowires and their sustainability for optoelectronic applications. Comparing Au- and self-assisted growth methods

    Energy Technology Data Exchange (ETDEWEB)

    Breuer, Steffen

    2011-09-28

    In this work the synthesis of GaAs nanowires by molecular beam epitaxy (MBE) using the vapour-liquid-solid (VLS) mechanism is investigated. A comparison between Au- and self-assisted VLS growth is at the centre of this thesis. While the Au-assisted method is established as a versatile tool for nanowire growth, the recently developed self-assisted variation results from the exchange of Au by Ga droplets and thus eliminates any possibility of Au incorporation. By both methods, we achieve nanowires with epitaxial alignment to the Si(111) substrates. Caused by differences during nanowire nucleation, a parasitic planar layer grows between the nanowires by the Au-assisted method, but can be avoided by the self-assisted method. Au-assisted nanowires grow predominantly in the metastable wurtzite crystal structure, while their self-assisted counterparts have the zincblende structure. All GaAs nanowires are fully relaxed and the strain arising from the lattice mismatch between GaAs and Si of 4.1 % is accommodated by misfit dislocations at the interface. Self-assisted GaAs nanowires are generally found to have vertical and non-polar side facets, while tilted and polar nanofacets were described for Au-assisted GaAs nanowires. We employ VLS nucleation theory to understand the effect of the droplet material on the lateral facets. Optoelectronic applications require long minority carrier lifetimes at room temperature. We fabricate GaAs/(Al,Ga)As core-shell nanowires and analyse them by transient photoluminescence (PL) spectroscopy. The results are 2.5 ns for the self-assisted nanowires as well as 9 ps for the Au-assisted nanowires. By temperature-dependent PL measurements we find a characteristic activation energy of 77 meV that is present only in the Au-assisted nanowires. We conclude that most likely Au is incorporated from the droplets into the GaAs nanowires and acts as a deep, non-radiative recombination centre.

  13. Development of new UV-vis spectroscopic microwave-assisted method for determination of glucose in pharmaceutical samples.

    Science.gov (United States)

    Mabood, Fazal; Hussain, Z; Haq, H; Arian, M B; Boqué, R; Khan, K M; Hussain, K; Jabeen, F; Hussain, J; Ahmed, M; Alharasi, A; Naureen, Z; Hussain, H; Khan, A; Perveen, S

    2016-01-15

    A new UV-Visible spectroscopic method assisted with microwave for the determination of glucose in pharmaceutical formulations was developed. In this study glucose solutions were oxidized by ammonium molybdate in the presence of microwave energy and reacted with aniline to produce a colored solution. Optimum conditions of the reaction including wavelength, temperature, and pH of the medium and relative concentration ratio of the reactants were investigated. It was found that the optimal wavelength for the reaction is 610 nm, the optimal reaction time is 80s, the optimal reaction temperature is 160°C, the optimal reaction pH is 4, and the optimal concentration ratio aniline/ammonium molybdate solution was found to be 1:1. The limits of detection and quantification of the method are 0.82 and 2.75 ppm for glucose solution, respectively. The use of microwaves improved the speed of the method while the use of aniline improved the sensitivity of the method by shifting the wavelength. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Investigation of Embedded Si/C System Exposed to a Hybrid Reaction of Centrifugal-Assisted Thermite Method.

    Science.gov (United States)

    Mahmoodian, Reza; Yahya, Rosiyah; Dabbagh, Ali; Hamdi, Mohd; Hassan, Mohsen A

    2015-01-01

    A novel method is proposed to study the behavior and phase formation of a Si+C compacted pellet under centrifugal acceleration in a hybrid reaction. Si+C as elemental mixture in the form of a pellet is embedded in a centrifugal tube. The pellet assembly and tube are exposed to the sudden thermal energy of a thermite reaction resulted in a hybrid reaction. The hybrid reaction of thermite and Si+C produced unique phases. X-ray diffraction pattern (XRD) as well as microstructural and elemental analyses are then investigated. XRD pattern showed formation of materials with possible electronic and magnetic properties. The cooling rate and the molten particle viscosity mathematical model of the process are meant to assist in understanding the physical and chemical phenomena took place during and after reaction. The results analysis revealed that up to 85% of materials converted into secondary products as ceramics-matrix composite.

  15. Investigation of Embedded Si/C System Exposed to a Hybrid Reaction of Centrifugal-Assisted Thermite Method.

    Directory of Open Access Journals (Sweden)

    Reza Mahmoodian

    Full Text Available A novel method is proposed to study the behavior and phase formation of a Si+C compacted pellet under centrifugal acceleration in a hybrid reaction. Si+C as elemental mixture in the form of a pellet is embedded in a centrifugal tube. The pellet assembly and tube are exposed to the sudden thermal energy of a thermite reaction resulted in a hybrid reaction. The hybrid reaction of thermite and Si+C produced unique phases. X-ray diffraction pattern (XRD as well as microstructural and elemental analyses are then investigated. XRD pattern showed formation of materials with possible electronic and magnetic properties. The cooling rate and the molten particle viscosity mathematical model of the process are meant to assist in understanding the physical and chemical phenomena took place during and after reaction. The results analysis revealed that up to 85% of materials converted into secondary products as ceramics-matrix composite.

  16. Simultaneous determination of plasma lopinavir and ritonavir by chemometrics-assisted spectrophotometry and comparison with HPLC method

    Directory of Open Access Journals (Sweden)

    Chutima Matayatsuk Phechkrajang

    2013-06-01

    Full Text Available Chemometrics-assisted spectrophotometry for the determination of two protease inhibitors, lopinavir and ritonavir, in plasma was evaluated. A set of calibration mixtures (calibration set was designed according to central composite design. The UV spectra obtained from the calibration set were subjected to partial least square regression (PLS-1 to construct the prediction models for lopinavir and ritonavir in unknown samples, which were then validated in a randomly selected set of synthetic mixtures of the drugs. An optimum model was obtained in the wavelength ranges of 215–249 nm and 240–279 nm with principal components 8 for both lopinavir and ritonavir respectively. The prediction models were used to analyse the two drugs in plasma and the results were compared with those obtained by high-performance liquid chromatography (HPLC. The PLS-1 model and the HPLC method were found to be comparable.

  17. Facile Synthesis of N-Doped BiOCl Photocatalyst by an Ethylenediamine-Assisted Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    Guihua Chen

    2015-01-01

    Full Text Available A nitrogen doped BiOCl (N-BiOCl photocatalyst was synthesized and characterized using an ethylenediamine-assisted hydrothermal method. The N-BiOCl sample demonstrated the same tetragonal crystal structure as the as-prepared pure BiOCl sample. SEM results indicated that N-BiOCl sample was self-assembled by nanoplates to provide an aggregated flower-like microstructure. Doped nitrogen was substituted for oxygen in the crystal lattice of BiOCl, causing a red shift for N-BiOCl sample compared to BiOCl sample. The N-BiOCl sample exhibited higher photocatalytic activity in the degradation of Rhodamine B under visible light than observed in BiOCl sample, and the stability of the sample was verified. Meanwhile, speculative causes for the enhancement in the photocatalytic activity of N-BiOCl sample were also proposed.

  18. Laser micro-machinability of borosilicate glass surface-modified by electric field-assisted ion-exchange method

    Science.gov (United States)

    Matsusaka, S.; Kobayakawa, T.; Hidai, H.; Morita, N.

    2012-08-01

    In order to improve the laser micro-machinability of borosilicate glass, the glass surface was doped with metal (silver or copper) ions by an electric field-assisted ion-exchange method. Doped ions drifted and diffused into the glass substrate under a DC electric field. The concentration of metal ions within the doped area was approximately constant because the ion penetration was caused by substitution between dopant metal and inherent sodium ions. Nanosecond ultraviolet laser irradiation of metal-containing regions produced flat, smooth and defect-free holes. However, the shapes of holes were degraded when the processed hole bottoms reached ion penetration depths. A numerical analysis of ionic drift-diffusion behaviour in glass material under an electric field was also carried out. The calculated results for penetration depth and ionic flux showed good agreement with the measured values.

  19. Kinetic analysis of lesions without mass effect on breast MRI using manual and computer-assisted methods.

    Science.gov (United States)

    Vag, Tibor; Baltzer, Pascal A T; Dietzel, Matthias; Zoubi, Ramy; Gajda, Mieczyslaw; Camara, Oumar; Kaiser, Werner A

    2011-05-01

    To analyse the kinetic characteristics of lesions without mass effect in dynamic breast MRI using manual and computer assisted methods. The enhancement pattern of 82 histopathologically verified lesions without mass effect (36 malignant, 46 benign) was evaluated on breast MRI using manual placement of a region of interest. Commercially available computer analysis software automatically assessed volume enhancement characteristics of a lesion voxelwise. Kinetic features evaluated included classification of the signal-intensity time curve as washout, plateau or persistent enhancement. Unlike manual ROI placement, computer-aided analysis demonstrated a significant difference in enhancement pattern between benign (washout: 32.6%, plateau: 32.6%, persistent: 34.8%) and malignant lesions without mass effect (77.1%, 8.6%, 14.3% respectively, P effect.

  20. Accurate quantification of tio2 nanoparticles collected on air filters using a microwave-assisted acid digestion method.

    Science.gov (United States)

    Mudunkotuwa, Imali A; Anthony, T Renée; Grassian, Vicki H; Peters, Thomas M

    2016-01-01

    Titanium dioxide (TiO(2)) particles, including nanoparticles with diameters smaller than 100 nm, are used extensively in consumer products. In a 2011 current intelligence bulletin, the National Institute of Occupational Safety and Health (NIOSH) recommended methods to assess worker exposures to fine and ultrafine TiO(2) particles and associated occupational exposure limits for these particles. However, there are several challenges and problems encountered with these recommended exposure assessment methods involving the accurate quantitation of titanium dioxide collected on air filters using acid digestion followed by inductively coupled plasma optical emission spectroscopy (ICP-OES). Specifically, recommended digestion methods include the use of chemicals, such as perchloric acid, which are typically unavailable in most accredited industrial hygiene laboratories due to highly corrosive and oxidizing properties. Other alternative methods that are used typically involve the use of nitric acid or combination of nitric acid and sulfuric acid, which yield very poor recoveries for titanium dioxide. Therefore, given the current state of the science, it is clear that a new method is needed for exposure assessment. In this current study, a microwave-assisted acid digestion method has been specifically designed to improve the recovery of titanium in TiO(2) nanoparticles for quantitative analysis using ICP-OES. The optimum digestion conditions were determined by changing several variables including the acids used, digestion time, and temperature. Consequently, the optimized digestion temperature of 210°C with concentrated sulfuric and nitric acid (2:1 v/v) resulted in a recovery of >90% for TiO(2). The method is expected to provide for a more accurate quantification of airborne TiO(2) particles in the workplace environment.

  1. Holmium laser enucleation versus photoselective vaporization for prostatic adenoma greater than 60 ml: preliminary results of a prospective, randomized clinical trial.

    Science.gov (United States)

    Elmansy, Hazem; Baazeem, Abdulaziz; Kotb, Ahmed; Badawy, Hesham; Riad, Essam; Emran, Ashraf; Elhilali, Mostafa

    2012-07-01

    To our knowledge we report the first single center, prospective, randomized study comparing holmium laser enucleation and high performance GreenLight™ prostate photoselective vaporization as surgical treatment of prostatic adenomas greater than 60 ml. A total of 80 patients with a large prostatic adenoma were randomly assigned to surgical treatment with holmium laser enucleation or photoselective vaporization. International Prostate Symptom Score, International Index of Erectile Function-15, maximum flow rate, post-void residual urine, serum prostate specific antigen and transrectal ultrasound volume were recorded. Patient baseline characteristics were similar for holmium laser enucleation and photoselective vaporization. Operative time and catheter removal time were almost equal in the 2 groups (p = 0.7 and 0.2, respectively). Eight vaporization cases were converted to transurethral prostate resection or holmium laser enucleation intraoperatively due to bleeding. A significantly higher maximum flow rate and lower post-void residual urine were noted in holmium laser cases during the entire followup (at 1 year each p = 0.02). However, no significant difference in International Prostate Symptom Score, quality of life or International Index of Erectile Function-15 was detected. Prostate volume and serum PSA decreased 78% and 88% in the holmium laser group, and 52% and 60% in the vaporization group, respectively. Holmium laser enucleation and photoselective vaporization are effective for lower urinary tract symptoms due to a large prostatic adenoma. Early subjective functional results (maximum flow rate and post-void residual urine) of holmium laser enucleation appear to be superior to those of photoselective vaporization. In our hands cases intended to be treated with photoselective vaporization were at 22% risk of conversion to another modality. This could reflect our determination to vaporize to the capsule in all vaporization cases. Copyright © 2012 American

  2. Mealtime assistance for older adults in hospital settings and rehabilitation units from the perspective of patients, families and healthcare professionals: a mixed methods systematic review

    OpenAIRE

    Edwards, Deborah; Carrier, Judith; Hopkinson, Jane

    2016-01-01

    Background: The prevalence of malnutrition for older adults (>65 years) in hospital and rehabilitation units has been reported as being as high as 60%; some older patients with good appetites do not receive sufficient nourishment because of inadequate feeding assistance. Mealtime assistance can therefore enhance nutritional intake, clinical outcomes and patient experience.\\ud \\ud \\ud Objectives: This mixed methods review sought to develop an aggregated synthesis of quantitative and qualitativ...

  3. Surfactant Assisted Stabilization of Carbon Nanotubes Synthesized by a Spray Pyrolysis Method

    Directory of Open Access Journals (Sweden)

    D. Mendoza-Cachú

    2017-01-01

    Full Text Available Surface modification of carbon nanotubes has been an interesting issue from a composites materials point of view. A nanotubes agglomeration has to be avoided to achieve a homogeneous dispersion in a composite matrix. In this research, we report on the synthesis of carbon nanotubes using a variant of the chemical vapor deposition technique known as spray pyrolysis method. X-ray diffraction (XRD, transmission electron microscopy (TEM, and scanning electron microscopy (SEM studies showed that the synthesized products had an aligned structure with low purity degree, high content of catalyst particles, and a smaller amount of amorphous carbon. A secondary method was applied, which involves an acidic treatment that dissolves contaminant particles to enhance the purity of the nanotubes. Microstructural analysis, which includes XRD and SEM, indicates an effective reduction of impurities. Dispersion of the nanotubes was assessed using different surfactants, such as sodium dodecyl-sulfate (SDS and ethylenediaminetetraacetic acid (EDTA. Finally, Raman spectroscopy, UV-Vis, and SEM techniques confirm that better results were obtained with EDTA. For EDTA and SDS surfactants, low concentrations of 0.3 mg/mL and 0.2 mg/mL were most efficient, respectively.

  4. Application of Assistive Computer Vision Methods to Oyama Karate Techniques Recognition

    Directory of Open Access Journals (Sweden)

    Tomasz Hachaj

    2015-09-01

    Full Text Available In this paper we propose a novel algorithm that enables online actions segmentation and classification. The algorithm enables segmentation from an incoming motion capture (MoCap data stream, sport (or karate movement sequences that are later processed by classification algorithm. The segmentation is based on Gesture Description Language classifier that is trained with an unsupervised learning algorithm. The classification is performed by continuous density forward-only hidden Markov models (HMM classifier. Our methodology was evaluated on a unique dataset consisting of MoCap recordings of six Oyama karate martial artists including multiple champion of Kumite Knockdown Oyama karate. The dataset consists of 10 classes of actions and included dynamic actions of stands, kicks and blocking techniques. Total number of samples was 1236. We have examined several HMM classifiers with various number of hidden states and also Gaussian mixture model (GMM classifier to empirically find the best setup of the proposed method in our dataset. We have used leave-one-out cross validation. The recognition rate of our methodology differs between karate techniques and is in the range of 81% ± 15% even to 100%. Our method is not limited for this class of actions but can be easily adapted to any other MoCap-based actions. The description of our approach and its evaluation are the main contributions of this paper. The results presented in this paper are effects of pioneering research on online karate action classification.

  5. Computer assisted strain-gauge plethysmography is a practical method of excluding deep venous thrombosis

    Energy Technology Data Exchange (ETDEWEB)

    Goddard, A.J.P.; Chakraverty, S.; Wright, J

    2001-01-01

    AIM: To evaluate a computed strain-gauge plethysmograph (CSGP) as a screening tool to exclude above knee deep venous thrombosis (DVT). METHODS: The first phase took place in the Radiology department. One hundred and forty-nine patients had both Doppler ultrasound and CSGP performed. Discordant results were resolved by venography where possible. The second phase took place in an acute medical admissions ward using a modified protocol. A further 173 patients had both studies performed. The results were collated and analysed. RESULTS: Phase 1. The predictive value of a negative CSGP study was 98%. There were two false-negative CSGP results (false-negative rate 5%), including one equivocal CSGP study which had deep venous thrombosis on ultrasound examination. Two patients thought to have thrombus on ultrasound proved not to have acute thrombus on venography. Phase 2. The negative predictive value of CSGP using a modified protocol was 97%. There were two definite and one possible false-negative studies (false-negative rate 4-7%). CONCLUSION: Computer strain-gauge plethysmograph can provide a simple, cheap and effective method of excluding lower limb DVT. However, its use should be rigorously assessed in each hospital in which it is used. Goddard, A.J.P., Chakraverty, S. and Wright, J. (2001)

  6. Efficacy of transurethral prostate enucleation by bipolar system for patients with benign prostatic hyperplasia.

    Science.gov (United States)

    Sato, Katsuhiko; Obinata, Daisuke; Funakoshi, Daigo; Saito, Fuminori; Takada, Shogo; Ito, Akiko; Murata, Yasutaka; Ashikari, Daisaku; Ikado, Yuichiro; Igarashi, Tomohiro; Matsui, Tsuyoshi; Mochida, Junichi; Yamanaka, Yataro; Yamaguchi, Kenya; Takahashi, Satoru

    2016-08-01

    The aim of this paper was to evaluate the efficacies and safety of transurethral prostate enucleation by bipolar system (TUEB) for the patients with benign prostatic hyperplasia (BPH). We prospectively evaluated clinical outcomes of TUEB in 55 patients with BPH from July 2005 to January 2011. Mean ages of the patients were 69.2 years. International Prostate Symptom Score (IPSS), IPSS-quality of life (IPSS-QOL) were assessed before and 12 months after surgery. Serum PSA, maximal flow rate (MFR), and post-void residual (PVR) were also evaluated before and 6 and 12 months after surgery. The median prostate volumes and resection volumes were 64.1 g (interquartile range [IQR]: 48-87) and 34.4 g (25-60.2), respectively. The median operation time was 138.0 min (100.2-169.2). Total IPSS scores and IPSS-QOL were significantly improved (from 24 [17-31] to 5 [2-8] points, and from 6 [5-6] to 2 [1-2] points, both P<0.001). MFR and PVR were significantly improved 6 and 12 months after TUEB (from 6.2 [3.9-8.3] to 15.1 [10.5-20.9], and 14.6 [10.2-20.5] mL/s, P<0.0001, and from 151.5 [81.5-284.7] to 16.5 [0-30.5], and 6.0 [0-41.0] mL, P<0.0001, respectively). Serum PSA also significantly decreased, 6 and 12 months (from 7.5 [4.7-9.8] to 1.1 [0.5-1.5], and 0.6 [0.3-1.9] ng/mL, P<0.0001). Although hemoglobin decreased after operation, no case experienced blood transfusion. Three episodes of urinary tract infections, 14 cases of mild stress urinary incontinence, 2 cases of urinary retention were occurred transiently with recovery within 1 month after surgery. We identified favorable efficacy and safety of TUEB. TUEB appears to be another possibility in the treatment of BPH.

  7. Does perioperative outcome of transurethral holmium laser enucleation of the prostate depend on prostate size?

    Science.gov (United States)

    Kuntz, Rainer M; Lehrich, Karin; Ahyai, Sascha

    2004-03-01

    In conventional transurethral resection of the prostate (TURP), perioperative morbidity resulting from causes such as blood loss and TUR syndrome increases with prostate size. Therefore, TURP is restricted to small and medium-sized glands. The present study aimed to find out whether perioperative parameters of holmium laser enucleation of the prostate (HoLEP) other than operation time and weight of resected tissue were dependent on prostate size. A total of 384 patients were treated with HoLEP (holmium:YAG laser, 2.0 J, 40 or 50 Hz, 80 or 100 W, 550-nm bare fiber) for acute removal of obstructing benign hyperplastic tissue. Among them, 111 patients (28.9%) had prostates of /=80 g (range 80-260) (group 3). The perioperative outcomes of the three groups were compared. A total of 346 patients completed the 1-month postoperative assessment. The mean prostate sizes were 31.8 g, 56 g, and 98.7 g for groups 1, 2, and 3 (P<0.0001 group 1 v group 2 v group 3). The mean resected tissue weight was 19.5 g v 34.4 g (P= 0.009) v 70.1 g (range 50-220) (P< 0.0001). The mean operation time was 64.3 v 84.2 (P= 0.009) v 118.4 minutes (P< 0.0001). The mean hemoglobin loss was 0.9 v 1.2 (NS) v 1.9 g/dL (P< 0.001). The overall correlation between hemoglobin loss and prostate size in all patients was very weak (r = 0.229) and just exceeded the level of significance (r = 0.2). In all three groups, the median postoperative catheter time was 1 day, and the median postoperative hospital stay was 2 days. The HoLEP resulted in an immediate and significant improvement of American Urological Association Symptom Scores, peak urinary flow rates, and postvoiding residual urine volumes (P< 0.0001) 1 month after the operation, without significant differences between the groups. The rate of complications was similar in all three groups. None of the patients needed blood transfusions. There were no perioperative deaths. In HoLEP, perioperative morbidity and postoperative micturition improvement do not

  8. Transurethral vapor enucleation and resection of the prostate with plasma vaporization button electrode for the treatment of benign prostatic hyperplasia: a feasibility study.

    Science.gov (United States)

    Xie, Liping; Mao, Qiqi; Chen, Hong; Qin, Jie; Zheng, Xiangyi; Lin, Yiwei; Wang, Xiao; Liu, Ben

    2012-10-01

    Various improvements and modifications to the surgical treatment of benign prostatic hyperplasia have emerged over the last decade. Most techniques often initially claimed superiority only to turn out to be mediocre with time. Holmium laser enucleation of the prostate has been associated with superior outcomes compared with transurethral resection of the prostate and demonstrated improvement in long-term outcomes, while its clinical use has limitations. We describe the first use of plasma vaporization button electrode combined with loop electrode for transurethral vapor enucleation and resection of the prostate.

  9. Leaching of silver from solid waste using ultrasound assisted thiourea method.

    Science.gov (United States)

    Oncel, M Salim; Ince, Mahir; Bayramoglu, Mahmut

    2005-02-01

    Thiourea leaching of precious metals such as gold and silver from ores has several advantages when compared with conventional cyanidation process. In recent years, the use of ultrasound in leaching processes is becoming increasingly popular in hydrometallurgy. This paper deals with combining these two techniques for silver leaching from solid waste of a cyanidation leach plant located in Kutahya, Turkey. The primary aim of this research is to assess the technical performance of the method. To achieve maximum leaching yield, eight process variables have been selected to estimate optimum process conditions by means of statistical factorial design and steepest ascent techniques. Laboratory-scale experiments showed that complete leaching of silver may be achieved by this process.

  10. A facile one-pot synthesis of polyaniline/magnetite nanocomposites by micelles-assisted method

    Science.gov (United States)

    Basavaiah, K.; Pavan Kumar, Y.; Prasada Rao, A. V.

    2013-10-01

    Magnetic nanocomposites based on polyaniline (PANI) and magnetite nanoparticles (Fe3O4 NPs) have been prepared by an in situ self-assembly method in presence of dodecylbenzene sulfonic acid (DBSA) as dopant as well as surfactant. Influence of the aniline to DBSA molar ratio on morphology, magnetic properties, and thermal stability of PANI/Fe3O4 NPs composites has been investigated. Spectroscopic results indicated the interaction between PANI nanorods and Fe3O4 NPs. Scanning electron microscopy and transmission electron microscopy images indicated that PANI rods were decorated with Fe3O4 NPs. Morphologies of nanocomposites were found to be critically dependent on molar ratios of organic acid to monomer. PANI nanorod/Fe3O4 NPs composites showed superparamagnetism and higher thermal stability with small mass fraction of Fe3O4 NPs.

  11. System and method for liquid extraction electrospray-assisted sample transfer to solution for chemical analysis

    Science.gov (United States)

    Kertesz, Vilmos; Van Berkel, Gary J.

    2016-07-12

    A system for sampling a surface includes a surface sampling probe comprising a solvent liquid supply conduit and a distal end, and a sample collector for suspending a sample collection liquid adjacent to the distal end of the probe. A first electrode provides a first voltage to solvent liquid at the distal end of the probe. The first voltage produces a field sufficient to generate electrospray plume at the distal end of the probe. A second electrode provides a second voltage and is positioned to produce a plume-directing field sufficient to direct the electrospray droplets and ions to the suspended sample collection liquid. The second voltage is less than the first voltage in absolute value. A voltage supply system supplies the voltages to the first electrode and the second electrode. The first electrode can apply the first voltage directly to the solvent liquid. A method for sampling for a surface is also disclosed.

  12. CAD AND CAE METHODS IN COMPUTER ASSISTED DESIGN OF WORM MESHES

    Directory of Open Access Journals (Sweden)

    Henryk SABINIAK

    2014-03-01

    Full Text Available One important feature of worm gear meshes is their complex geometry and now the application of computer aided design allows for mapping their real construction patterns with great affinity. Modelling of worm meshes with the use of CAD (Computer Aided Design and CAE (Computer Aided Engineering methods enables tracking the design process from the very beginning, i.e. from the concept or specific assumptions, until the final stage only by using appropriate software, thereby lowering the costs and eliminating common errors. Owing to this, just before the manufacturing process of the first meshing elements begins, one can conduct geometric analysis and correct or modify working profiles of the teeth in order to improve the operation properties of future gear. Visualizing such a geometric modelling process can also serve didactic purposes when used in education of future engineers, technologists and operators of this type of gears.

  13. Avaliação das dimensões orbitárias de coelhos após enucleação e evisceração Rabbit orbital measure evaluations after enucleation and evisceration

    Directory of Open Access Journals (Sweden)

    Claudia Akemi Shiratori

    2005-04-01

    Full Text Available OBJETIVO: Avaliar o volume orbitário de coelhos recém-nascidos, enucleados ou eviscerados, sem ou com reposição do volume orbitário por esferas de polietileno poroso de 10 mm, usando a tomografia computadorizada, o teste de deslocamento de água e a medida da abertura orbitária por Image-J. MÉTODOS: Estudo experimental, usando 48 coelhos albinos, com idade de 42 dias, submetidos à evisceração ou enucleação, com implante de esfera de polietileno poroso de 10 mm de diâmetro ou sem implante. O lado esquerdo não foi submetido à cirurgia e foi usado como controle. Após 1, 3 e 6 meses, foram sacrificados quatro animais de cada grupo. Os crânios foram preparados para estudo do osso seco por maceração da peça, e analisados em relação ao volume orbitário por tomografia computadorizada, teste de deslocamento de água e avaliação da abertura orbitária usando o software Image-J. RESULTADOS: O volume das órbitas enucleadas ou evisceradas, com ou sem o uso do implante não apresentaram diferenças estatisticamente significativas (pPURPOSE: To assess orbital volume in weaned rabbits, submitted to enucleation and evisceration, with orbital volume reconstitution using porous polyethylene (PP spheres, analyzed by computed tomography, by the water displacement test and external orbital measures using Image-J software. METHODS: This experimental randomized study consisted of 48 albino rabbits, with 42 days of life, submitted to enucleation (EN and evisceration (EV, with (WI or without (NI implantation of a 10 mm PP sphere. After 1 month (M1, 3 months (M2 and 6 months (M3, 4 animals of each group were sacrificed. The skulls were prepared for dry bone study using the maceration technique. The orbital volume was calculated by computed tomography and by the water displacement test. The results suggest that orbital development in rabbits above 42 days of life is not different regarding evisceration or enucleation, or when an orbital implant is

  14. Hysteroscopic enucleation in toto of submucous type 2 myomas: long-term follow-up in women affected by menorrhagia.

    Science.gov (United States)

    Saccardi, Carlo; Conte, Lorena; Fabris, Alberta; De Marchi, Francesca; Borghero, Angela; Gizzo, Salvatore; Litta, Pietro

    2014-01-01

    To evaluate long-term efficacy of type 2 myoma enucleation in toto. Longitudinal retrospective study (Canadian Task Force classification II-2). University obstetrics and gynecology clinic. One hundred twelve women with menorrhagia and at least 1 type 2 submucous myoma who underwent hysteroscopic myoma enucleation in toto. Clinical long-term follow-up. Success of the procedure and influence of myoma characteristics on recurrence of menorrhagia were evaluated. Mean (SD) follow-up was 58.4 (19.1) months. The success of the procedure was 88.4% (99 patients). Seventeen patients (15.2%) underwent a 2-step procedure. Among patients with relapsed menorrhagia, 10 (8.9%) underwent a repeat operation. Statistical analysis showed that number and diameter of myomas did not influence the outcome. Localization in the posterior wall of the uterus, compared with other sites, was associated with a higher percentage of resolution of menstrual symptoms (p = .03). There was no significant relationship between myomas features and risk of symptom recurrence during follow-up. The 2-step myomectomy was performed in patients with myomas >30 mm in diameter (p menorrhagia. Copyright © 2014 AAGL. Published by Elsevier Inc. All rights reserved.

  15. Investigation of diamond wheel topography in Elliptical Ultrasonic Assisted Grinding (EUAG) of monocrystal sapphire using fractal analysis method.

    Science.gov (United States)

    Wang, Qiuyan; Zhao, Wenxiang; Liang, Zhiqiang; Wang, Xibin; Zhou, Tianfeng; Wu, Yongbo; Jiao, Li

    2018-03-01

    The wear behaviors of grinding wheel have significant influence on the work-surface topography. However, a comprehensive and quantitative method is lacking for evaluating the wear conditions of grinding wheel. In this paper, a fractal analysis method is used to investigate the wear behavior of resin-bonded diamond wheel in Elliptical Ultrasonic Assisted Grinding (EUAG) of monocrystal sapphire, and a series of experiments on EUAG and conventional grinding (CG) are performed. The results show that the fractal dimension of grinding wheel topography is highly correlated to the wear behavior, i.e., grain fracture, grain pullout, and wheel loading. An increase in cutting edge density on the wheel surface results in an increase of the fractal dimension, but an increase in the grain pullout and wheel loading results in a decrease in the fractal dimension. The wheel topography in EUAG has a higher fractal dimension than that in CG before 60 passes due to better self-sharpening behavior, and then has a smaller fractal dimension because of more serious wheel loadings after 60 passes. By angle-dependent distribution analysis of profile fractal dimensions, the wheel surface topography is transformed from isotropic to anisotropic. These indicated that the fractal analysis method could be further used in monitoring of a grinding wheel performance in EUAG. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. A modular video streaming method for surgical assistance in operating room networks.

    Science.gov (United States)

    Voruganti, Arun Kumar Raj; Mayoral, Rafael; Vazquez, Adrian; Burgert, Oliver

    2010-09-01

    Continuous video is used with increasing frequency in the operating room for minimally invasive laparoscopic and endoscopic procedures. Video data communication in the OR requires device interoperability, efficient data transfer methods, and specialized IT infrastructure. A framework for digital video communication based on a two channel client-server architecture was developed and tested. One channel is used for stream handling and the second channel is used for data streaming. A video stream description (VSD) specification is defined to negotiate video stream characteristics and ensure semantic interoperability. Quality assessment of the streamed data employs an image-based structural quality measure called the Structural Similarity (SSIM) Index. By introducing the stream description and a quality metric, the stream parameters can be modified as needed. The video communication framework ensures interoperability by defining interfaces for each of the streaming architecture modules. To prove the framework's feasibility, two prototype applications were developed and performance tests were performed on a dedicated OR network. The results showed acceptable network performance for streaming video in the OR network under clinically realistic conditions. An OR video communications framework was developed that uses existing OR network infrastructure as an economical alternative to dedicated integrated OR solutions. This framework provides functional and semantic interoperability among imaging modalities for continuous video data communication.

  17. Reformer assisted lean NO.sub.x catalyst aftertreatment system and method

    Science.gov (United States)

    Kalyanaraman, Mohan [Media, PA; Park, Paul W [Peoria, IL; Ragle, Christie S [Havana, IL

    2010-06-29

    A method and apparatus for catalytically processing a gas stream passing therethrough to reduce the presence of NO.sub.x therein, wherein the apparatus includes a first catalyst composed of a silver-containing alumina that is adapted for catalytically processing the gas stream at a first temperature range, a second catalyst composed of a copper-containing zeolite located downstream from the first catalyst, wherein the second catalyst is adapted for catalytically processing the gas stream at a lower second temperature range relative to the first temperature range, a hydrocarbon compound for injection into the gas stream upstream of the first catalyst to provide a reductant, and a reformer for reforming a portion of the hydrocarbon compound into H.sub.2 and/or oxygenated hydrocarbon for injection into the gas stream upstream of the first catalyst. The second catalyst is adapted to facilitate the reaction of reducing NOx into N.sub.2, whereby the intermediates are produced via the first catalyst reacting with NOx and hydrocarbons.

  18. Development of Deal - and Journal- level Metrics and Methods Assists Librarians to Evaluate Big Deals

    Directory of Open Access Journals (Sweden)

    Kathleen Reed

    2014-07-01

    Full Text Available Objective – To assess the value of aggregated journal packages (Big Deals and to select individual journal titles for continued subscription should a deal be cancelled. Design – Case study. Setting – Doctoral research university library in the United States of America. Subjects – Three anonymous Big Deals. Methods – The authors define metrics at two levels (deal and journal to evaluate Big Deal packages. The metrics rely heavily on the COUNTER JR1 metric Successful Full-Text Article Request (SFTAR. Main Results – The authors found that while 30% of journals provide 80% of SFTARs, the cost of subscribing to these journals individually would not save significant sums of money. Additionally, they speculate that library users would increase the number of interlibrary loan requests to access the 20% of SFTARs that would be inaccessible if a Big Deal was cut, amounting to increased costs. Main Results – The authors found that while 30% of journals provide 80% of SFTARs, the cost of subscribing to these journals individually would not save significant sums of money. Additionally, they speculate that library users would increase the number of interlibrary loan requests to access the 20% of SFTARs that would be inaccessible if a Big Deal was cut, amounting to increased costs.

  19. Optical and morphological properties of ZnO- and TiO2-derived nanostructures synthesized via a microwave-assisted hydrothermal method

    CSIR Research Space (South Africa)

    Moloto, N

    2012-01-01

    Full Text Available A microwave-assisted hydrothermal method was used to synthesize ZnO and TiO2 nanostructures. The experimental results show that the method resulted in crystalline monodispersed ZnO nanorods that have pointed tips with hexagonal crystal phase. TiO2...

  20. Holmium Laser Enucleation of the Prostate is Effective in the Treatment of Symptomatic Benign Prostatic Hyperplasia of Any Size Including a Small Prostate

    Science.gov (United States)

    Lee, Min Ho; Yang, Hee Jo; Kim, Doo Sang; Lee, Chang Ho

    2014-01-01

    Purpose Although transurethral resection of the prostate (TURP) is considered the standard surgical treatment for benign prostatic hyperplasia (BPH), Holmium laser enucleation of the prostate (HoLEP) is replacing TURP. We compared TURP with HoLEP with matching for prostate size. Materials and Methods We retrospectively reviewed the medical charts of patients who underwent TURP and HoLEP performed by one surgeon at our institute. All patients were categorized into 3 groups on the basis of prostate size (group 1, 80 g), and 45 patients were selected for each method. Results No major intraoperative complications were encountered. The mean resected tissue weight was 6.3, 18.3, and 28.0 g for groups 1, 2, and 3, respectively, for TURP and 8.7, 25.0, and 39.8 g, respectively, for HoLEP. The mean operation time was 51.8, 89.3, and 101.9 minutes for TURP and 83.6, 122.8, and 131.2 minutes for HoLEP in groups 1, 2, and 3, respectively. HoLEP had better resection efficacy than TURP for any size prostate, but there was no statistical difference between the methods. Both methods resulted in an immediate and significant improvement of International Prostate Symptom Score, peak urinary flow rates, and postvoid residual urine volume. Conclusions HoLEP is effective for BPH treatment, regardless of prostate size, even in a small prostate. The perioperative morbidity of HoLEP is also comparable to that of TURP. PMID:25405016

  1. Hematocrit as a simple method to predict and manage ovarian hyperstimulation syndrome in assisted reproduction

    Directory of Open Access Journals (Sweden)

    Taswin Kaur

    2015-01-01

    Full Text Available Aim: The aim was to analyze the hematocrit levels in cases of ovarian hyperstimulation syndrome (OHSS, syndrome occurring during in-vitro fertilization (IVF, and study its role as a prognostic indicator. Subjects and Methods: Two years data of 66 women at high risk for developing OHSS was analyzed. Twenty-seven women who developed OHSS were further analyzed based on their hematocrit levels on the day of oocyte pick-up (OPU and the day of embryo transfer (ET to see if there was a prognostic trend. Results: Of the total 225 IVF cases, 66 were deemed high risk for developing OHSS. Twenty-seven of these developed OHSS (40.9%. Of these 27, 21 (77.8% had a hematocrit >35% on the day of OPU. The mean hematocrit in women developing OHSS on the day of OPU was 37.39% (standard deviation [SD] 2.66 as against 35.97% (2.80 in those not developing OHSS. This difference was statistically significant (P = 0.043. On the day of ET, 23/27 (85.8% who developed OHSS had a hematocrit of >35%. The mean hematocrit was 39.29% (SD 3.85 in those who developed OHSS as against 34.7% (2.88 in those who did not. This difference (4.85 was statistically significant (P 35%. Those who required cancellation of ET had a hematocrit of >35% on the day of ET or showed a significant increase of 3% from OPU to ET.

  2. The barrier method as a new tool to assist in career selection: covert observational study.

    Science.gov (United States)

    McCain, R Scott; Harris, Andrew R; McCallion, Kevin; Campbell, W Jeffrey; Kirk, Stephen J

    2010-12-15

    To determine if senior doctors' parking habits and skills are associated with clinical specialty and, if so, whether observation of junior doctors' parking could provide guidance in choice of specialty. Covert observational study. Pass-card controlled consultants' car park (parking lot), December 2009. 103 consultants entering the car park on three consecutive mornings. The outcomes were specialty and sex of the consultants, manner of approaching the barrier (pass-card ready or not), and time taken to park, exit the vehicle, and walk to a designated point. Approaches to the barrier and parking were recorded for 103 consultants (79 men, 24 women): 28 anaesthetists (22 men, six women), 29 physicians (internists, 18 men, 11 women), 14 radiologists (nine men, five women), and 32 surgeons (30 men, two women). The manner of approaching the barrier (card ready) differed by specialty but not by sex. The total time taken to park (seconds) differed significantly between specialties: surgery (median 68, interquartile range 61-71 seconds), anaesthesia (82, 76-91), radiology (86, 70-103), and general medicine (112, 96-136). The time taken to park was overall longer among women, but this was explained by their specialty (men and women matched by specialty did not differ). The total time taken to park and manner of approaching the barrier to gain entry to the car park differed across specialties. Surgical consultants were fastest, followed by consultant anaesthetists and consultant radiologists, with physicians slowest. Sex was not an influencing factor. If reproducible in studies of a similar nature the "barrier method" could allow for a low cost means of guiding junior doctors in career selection.

  3. Model-assisted measuring method for periodical sub-wavelength nanostructures.

    Science.gov (United States)

    Alexe, Gabriela; Tausendfreund, Andreas; Stöbener, Dirk; Fischer, Andreas

    2018-01-01

    This paper describes a scatterometry approach designed by simulations for the in-line characterization of sub-wavelength sinusoidal gratings, which are formed on a transparent foil in a roll-to-roll procedure. Currently used methods are based on series of in situ measurements of the specular optical response at different incident angles or wavelengths for acquiring dimensional information on the gratings. The capability of single measurements of the first diffraction maxima at a fixed incident angle and wavelength to accurately measure the height of the sub-wavelength sinusoidal gratings is investigated in this work. The relation between the scattered powers of the diffraction maxima and the grating height is extracted from light scattering simulations, i.e., the inverse problem is solved. Optimal setup parameters for the measurement of grating heights ranging from 100 nm to 300 nm are derived from simulations. Limits of measurability and the measurement uncertainty are evaluated for different instrumentation and simulation parameters. When using laser light in the visible wavelength range, the measurement uncertainty is physically limited by the photon shot noise to the picometer range, but the systematic contributions dominate the uncertainty. As a result, the measurement uncertainty for the grating height is estimated to ≤12  nm, with a potential for <4  nm. Large-area scanning measurements performed offline and reference atomic force microscopy measurements verify the sensitivity of the presented measurement approach for identifying local variations of the spatial surface properties. Depending on the chosen detection system, sampling rates up to the MHz range are feasible, meeting the requirements of in-line process control of the roll-to-roll production process.

  4. Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods

    Directory of Open Access Journals (Sweden)

    Mahmoud Mohamed Issa

    2013-01-01

    Full Text Available Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.

  5. Influencing factors and kinetics analysis on the leaching of iron from boron carbide waste-scrap with ultrasound-assisted method.

    Science.gov (United States)

    Li, Xin; Xing, Pengfei; Du, Xinghong; Gao, Shuaibo; Chen, Chen

    2017-09-01

    In this paper, the ultrasound-assisted leaching of iron from boron carbide waste-scrap was investigated and the optimization of different influencing factors had also been performed. The factors investigated were acid concentration, liquid-solid ratio, leaching temperature, ultrasonic power and frequency. The leaching of iron with conventional method at various temperatures was also performed. The results show the maximum iron leaching ratios are 87.4%, 94.5% for 80min-leaching with conventional method and 50min-leaching with ultrasound assistance, respectively. The leaching of waste-scrap with conventional method fits the chemical reaction-controlled model. The leaching with ultrasound assistance fits chemical reaction-controlled model, diffusion-controlled model for the first stage and second stage, respectively. The assistance of ultrasound can greatly improve the iron leaching ratio, accelerate the leaching rate, shorten leaching time and lower the residual iron, comparing with conventional method. The advantages of ultrasound-assisted leaching were also confirmed by the SEM-EDS analysis and elemental analysis of the raw material and leached solid samples. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Students’ Knowledge Comprehension after Implementation of Live Conventional Demonstration, Video Teaching and Video-Assisted Instruction Methods in Endodontic Practice

    Science.gov (United States)

    Mohammadzadeh Akhlaghi, Nahid; Khalilak, Zohreh; Vatanpour, Mehdi; Moshari, Amirabbas; Ghaffari, Saman; Namazikhah, Mohammad Sadegh

    2017-01-01

    Introduction: Video-assisted clinical instruction (VACID) has been found to be a beneficial teaching tool for various fields in dentistry. The aim of this interventional study was to compare the efficacy of live conventional demonstration (CD), video teaching, and VACID (video with explanation) methods in teaching of root canal treatment to undergraduate dental students. Methods and Materials: Forty-two undergraduate senior dental students participated in this study. The students experienced this course for the first time and were randomly divided into three groups (n=14). Group A attended live CD on a patient; group B watched a professionally produced demonstration video without any verbal explanation during 1 h; and finally group C watched the same video alongside live explanation by a mentor during the 1.5 h (VACID). The whole process was performed by an experienced endodontist on maxillary central incisors. All of The students carried out a multiple choice question exam to evaluate their comprehension. The mean score of the experimental groups were compared using ANOVA test and multiple comparisons were carried out with Tamhane test. The level of significance was set at 0.05. Results: There was significant difference among three groups according to the ANOVA test (Pendodontic training in undergraduate dental students. PMID:28512486

  7. Development of a computer-assisted forensic radiographic identification method using the lateral cervical and lumbar spine.

    Science.gov (United States)

    Derrick, Sharon M; Raxter, Michelle H; Hipp, John A; Goel, Priya; Chan, Elaine F; Love, Jennifer C; Wiersema, Jason M; Akella, N Shastry

    2015-01-01

    Medical examiners and coroners (ME/C) in the United States hold statutory responsibility to identify deceased individuals who fall under their jurisdiction. The computer-assisted decedent identification (CADI) project was designed to modify software used in diagnosis and treatment of spinal injuries into a mathematically validated tool for ME/C identification of fleshed decedents. CADI software analyzes the shapes of targeted vertebral bodies imaged in an array of standard radiographs and quantifies the likelihood that any two of the radiographs contain matching vertebral bodies. Six validation tests measured the repeatability, reliability, and sensitivity of the method, and the effects of age, sex, and number of radiographs in array composition. CADI returned a 92-100% success rate in identifying the true matching pair of vertebrae within arrays of five to 30 radiographs. Further development of CADI is expected to produce a novel identification method for use in ME/C offices that is reliable, timely, and cost-effective. © 2014 American Academy of Forensic Sciences.

  8. Bioinspired synthesis of high-performance nanocomposite imprinted membrane by a polydopamine-assisted metal-organic method.

    Science.gov (United States)

    Wu, Yilin; Liu, Xinlin; Cui, Jiuyun; Meng, Minjia; Dai, Jiangdong; Li, Chunxiang; Yan, Yongsheng

    2017-02-05

    Significant efforts have been focused on the functionalization and simplification of membrane-associated molecularly imprinted materials, which can rapidly recognize and separate specific compound. However, issues such as low permselectivity and unstable composite structures are restricting it from developing stage to a higher level. In this work, with the bioinspired design of polydopamine (pDA)-assisted inorganic film, we present a novel molecular imprinting strategy to integrate multilevel nanocomposites (Ag/pDA) into the porous membrane structure. The molecularly imprinted nanocomposite membranes were then obtained through an in situ photoinitiated ATRP method by using tetracycline (TC) as the template molecule. Importantly, attributing to the formation of the Ag/pDA-based TC-imprinted layers, largely enhance TC-rebinding capacity (35.41mg/g), adsorption selectivity and structural stability (still maintained 92.1% of the maximum adsorption capacity after 10 cycling operations) could been easily achieved. Moreover, largely enhanced permselectivity performance toward template molecule (the permeability factor β values were also more than 5.95) was also obtained. Finally, all synthesis methods were conducted in aqueous solution at ambient temperature, which was environmental friendly for scaling up without causing pollution. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method.

    Science.gov (United States)

    Jing, Chang-Liang; Dong, Xiao-Fang; Tong, Jian-Ming

    2015-08-26

    Ultrasonic-assisted extraction (UAE) was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology (RSM), based on a four-factor, five-level central composite design (CCD), was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) methods. The results showed good fit with the proposed models for the total flavonoid extraction (R² = 0.9849), for the antioxidant extraction assayed by ABTS method (R² = 0.9764), and by DPPH method (R² = 0.9806). Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL/g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL/g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL/g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa.

  10. Vaporize, anatomically vaporize or enucleate the prostate? The flexible use of the GreenLight laser.

    Science.gov (United States)

    Cindolo, Luca; Ruggera, Lorenzo; Destefanis, Paolo; Dadone, Claudio; Ferrari, Giovanni

    2017-03-01

    GreenLight laser has gained increasing acceptance as a less invasive treatment for lower urinary tract symptoms due to benign prostatic hyperplasia (BPH/LUTS). Three surgical options were developed: standard photovaporization (PVP), anatomical PVP and GreenLight enucleation of prostate (GreenLEP); however, literature lacks a direct comparison among the procedures. Aim of the present study is to compare the three techniques in a multicentre series of patients. Data were collected from consecutive patients with indication to surgical management of BPH/LUTS in five institutions. Patients underwent standard PVP, anatomical PVP or GreenLEP according to surgeon preferences. Standard parameters associated with transurethral prostate surgery were documented prior surgery and during the follow-up. Patients' perception of improvement was measured using a single-item scale. Early (within first 30 post-operative days) and delayed post-operative complications were recorded. Descriptive statistics, univariate and multivariate analysis were used. We evaluate 367 consecutive patients (mean age 69.1 years). Median prostate size and PSA were 68 ml (IQR 50-90) and 2.8 ng/ml (IQR 1.7-4.3), respectively. The median operative time and applied energy were 60 min (IQR 45-75) and 250 kJ (IQR 160-364). Catheterization time and median post-operative stay were 1 and 2 days. No patient was transfused. The overall median Q max values increased for 8-19 ml/s (p < 0.05), median International Prostate Symptoms Score decreased from 24 to 7 (p < 0.05). A total of 7.4% urinary retention, 33.4% bothersome storage symptoms, 2.5% short-term stress incontinence were recorded. Three heart attacks, one pulmonary embolism and one death occurred. Prostate volume was a predictive factor for post-operative storage symptoms (p = 0.049). Nine percentage of patients experienced long-term complications (4, 0.9 and 0.9% of urethral stricture, bladder neck contracture and prostatic fossa sclerosis

  11. Mealtime assistance for older adults in hospital settings and rehabilitation units from the perspective of patients, families and healthcare professionals: a mixed methods systematic review.

    Science.gov (United States)

    Edwards, Deborah; Carrier, Judith; Hopkinson, Jane

    2016-09-01

    The prevalence of malnutrition for older adults (>65 years) in hospital and rehabilitation units has been reported as being as high as 60%; some older patients with good appetites do not receive sufficient nourishment because of inadequate feeding assistance. Mealtime assistance can therefore enhance nutritional intake, clinical outcomes and patient experience. This mixed methods review sought to develop an aggregated synthesis of quantitative and qualitative data on assistance at mealtimes for older adults in hospital settings and rehabilitation units to determine current practices, what practices work, and the perceptions of patients, families, and healthcare professionals of mealtime assistance. TYPES OF PARTICIPANTS: Participants included older adults (65 years and over) in hospital settings, including rehabilitation units. The review focused on interventions for mealtime assistance, observed mealtime assistance, or discussed experiences of mealtime assistance with patients, families and healthcare professionals. The review included qualitative, quantitative and mixed methods studies. The outcomes of interest were the effectiveness of mealtime assistance initiatives and experiences of assistance at mealtimes. The search strategy identified studies from seven databases published between 1998 and 2015. Methodological quality of studies was independently assessed by two reviewers using standardized Joanna Briggs Institute critical appraisal instruments. Standardized Joanna Briggs Institute data extraction tools were used. Synthesis of the findings was reached through discussion. The results of quantitative studies could not be statistically pooled because of heterogeneity and are presented in narrative form. The results are presented as three aggregated mixed methods syntheses. A total of 21 publications (19 studies) were included: 11 quantitative, five qualitative and three mixed method studies. Two studies were conducted in rehabilitation units, and 17 in

  12. [Comparison of 2 µm continuous-wave laser enucleation of the prostate and transurethral resection of the prostate for benign prostatic hyperplasia].

    Science.gov (United States)

    Shao, Jin-kai; Wang, Yu-bin; Lü, Yong-an; Li, Xiao-dong

    2012-02-01

    To compare the safety and efficacy of RevoLix 120 W 2 µm continuous-wave (cw) laser enucleation of the prostate with transurethral resection of prostate (TURP) in patients with symptomatic benign prostatic hyperplasia (BPH). And to evaluate clinical value of 120 W 2 µm cw laser enucleation. All 168 patients with BPH underwent 2 µm cw laser enucleation (n = 88) or TURP (n = 80) between January 2010 and January 2011. The operative time, drop in hemoglobin, drop in serum sodium, indwelling catheterization time and operative complications were recorded. International prostate symptom score (IPSS), quality of life (QOL), urinary peak flow rate (Qmax) and post-voiding residual urine (PVR) were also compared. The mean operative time was slightly longer in the 2 µm laser group ((63.2 ± 21.6) min) than the TURP group ((59.4 ± 18.6) min) (P > 0.05). Transfusions were not necessary in 2 µm laser group. Catheter indwelling time were (1.8 ± 0.6) days vs. (3.5 ± 2.6) days in 2 µm laser group than in TURP group (t = 3.912, P enucleation is an novel excellent treatment for BPH as well as TURP, and has the advantage of significantly less blood loss, shorter hospitalization, shorter catheter indwelling time and rapid recovery after surgery.

  13. Assistance at mealtimes in hospital settings and rehabilitation units for patients (>65years) from the perspective of patients, families and healthcare professionals: A mixed methods systematic review.

    Science.gov (United States)

    Edwards, Deborah; Carrier, Judith; Hopkinson, Jane

    2017-04-01

    Malnutrition is one of the key issues affecting the health of older people (>65years). With an aging population the problem is expected to increase further since the prevalence of malnutrition increases with age. Studies worldwide have identified that some older patients with good appetites do not receive sufficient nourishment because of inadequate feeding assistance. Mealtime assistance can enhance nutritional intake, clinical outcomes and patient experience. To determine the effectiveness of meal time assistance initiatives for improving nutritional intake and nutritional status for older adult patients (>65years) in hospital settings and rehabilitation units. The review also sought to identify and explore the perceptions and experiences of older adult patients and those involved with their care. Mixed methods systematic review. A search of electronic databases to identify published studies (CINAHL, MEDLINE, British Nursing Index, Cochrane Central Register of Controlled Trials, EMBASE, PsychINFO, Web of Science (1998-2015) was conducted. Relevant journals were hand-searched and reference lists from retrieved studies were reviewed. The search was restricted to English language papers. The key words used were words that described meal time assistance for adult patients in hospital units or rehabilitation settings. The review considered qualitative, quantitative and mixed methods studies that included interventions for mealtime assistance, observed mealtime assistance or discussed experiences of mealtime assistance with staff, patients, relatives, volunteers or stakeholders. Extraction of data was undertaken independently by two reviewers. A further two reviewers assessed the methodological quality against agreed criteria. Twenty one publications covering 19 studies were included. Three aggregated mixed methods syntheses were developed: 1) Mealtimes should be viewed as high priority. 2a) Nursing staff, employed mealtime assistants, volunteers or relatives

  14. Bipolar Plasma Enucleation of the Prostate (B-TUEP) in Benign Prostate Hypertrophy Treatment: 3-Year Results.

    Science.gov (United States)

    Giulianelli, Roberto; Gentile, Barbara Cristina; Mirabile, Gabriella; Albanesi, Luca; Tariciotti, Paola; Rizzo, Giorgio; Buscarini, Maurizio; Falavolti, Cristina

    2017-09-01

    To investigate numerous endoscopic techniques that have been described for the treatment of benign prostate enlargement. Plasma-button enucleation of the prostate (B-TUEP) is a successful treatment option because the large surface creates a fast enucleation process, vaporization, and concomitant hemostasis. The aim of this study was to evaluate the efficacy of bipolar button electrode transurethral adenoma enucleation (B-TUEP) in saline solution. The second end point was to determine the change of International Prostate Symptom Score (IPSS), post-void residual urine, International Index of Erectile Function, transrectal ultrasound gland volume evaluation, and prostate-specific antigen. Between July 2011 and March 2012, 50 consecutive patients underwent B-TUEP in our facility, all performed by a single surgeon (R.G.). All patients were preoperatively assessed with maximum urinary flow rate, single-question quality of life assessment, IPSS and the International Index of Erectile Function questionnaires, transrectal ultrasound gland volume evaluation, prostate-specific antigen, and post-void residual urine. We observed a significant improvement at 12, 24, and 36 months in terms of maximum urinary flow rate (22.3 ± 4.74 mL/s, 23.2 ± 0.30 mL/s, and 23.6 ± 1.26 mL/s, respectively, P prostate-specific postoperative antigen levels were 0.76 ± 0.61 ng/mL, 0.7 ± 0.51 ng/mL, and 0.62 ± 0.18 ng/mL, at 12, 24, and 36 months, respectively. Two patients (4%) had persistent bladder outlet obstruction requiring reoperation. After 3-year follow-up, B-TUEP represents an effective, durable, and safe form of surgical intervention. B-TUEP is an alternative treatment for symptomatic benign prostate enlargement. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Comparison of survival among eligible patients not enrolled versus enrolled in the Collaborative Ocular Melanoma Study (COMS) randomized trial of pre-enucleation radiation of large choroidal melanoma.

    Science.gov (United States)

    Gilson, Marta M; Diener-West, Marie; Hawkins, Barbara S

    2007-01-01

    To compare survival between patients enrolled in the Collaborative Ocular Melanoma Study (COMS) randomized trial of pre-enucleation radiation therapy (PERT) for large choroidal melanoma and eligible patients who did not enroll. COMS clinical center personnel prospectively reported to the COMS Coordinating Center all patients with choroidal melanoma examined between November 1986 and December 1994. Deaths of enrolled patients were reported prospectively by clinical center personnel. In a COMS ancillary study, we retrospectively searched medical records of participating clinical centers, the Social Security Death Index, and the National Death Index to determine vital status of eligible patients not enrolled. Cox proportional hazards analysis was used to compare survival within 10 years of baseline reporting and before July 31, 2000, of enrolled patients versus eligible patients not enrolled. Clinical centers that received local institutional review board approval to participate in this ancillary study prospectively reported on 129 of 299 eligible patients not enrolled in the COMS PERT trial. The baseline characteristics of the 129 patients included in this ancillary study were similar to those of the 170 patients not included; 73 patients were reported as deceased. Previously identified prognostic covariates, i.e., age and longest tumor diameter, were confirmed to predict survival in both enrolled patients and eligible patients not enrolled; trial enrollment was not predictive. After adjusting for prognostic covariates and stratifying by clinical center, the estimated hazard ratio (enrolled vs. not-enrolled) was 1.12 (95% confidence interval: 0.83 to 1.51). The results of the COMS PERT trial should be generalizable to all patients with choroidal melanoma meeting the eligibility criteria for that trial. While the methods we used may not be generalizable to all clinical trials because of unique features of the COMS, other researchers may be able to use similar methods

  16. The order and priority of research and design method application within an assistive technology new product development process: a summative content analysis of 20 case studies.

    Science.gov (United States)

    Torrens, George Edward

    2018-01-01

    Summative content analysis was used to define methods and heuristics from each case study. The review process was in two parts: (1) A literature review to identify conventional research methods and (2) a summative content analysis of published case studies, based on the identified methods and heuristics to suggest an order and priority of where and when were used. Over 200 research and design methods and design heuristics were identified. From the review of the 20 case studies 42 were identified as being applied. The majority of methods and heuristics were applied in phase two, market choice. There appeared a disparity between the limited numbers of methods frequently used, under 10 within the 20 case studies, when hundreds were available. Implications for Rehabilitation The communication highlights a number of issues that have implication for those involved in assistive technology new product development: •The study defined over 200 well-established research and design methods and design heuristics that are available for use by those who specify and design assistive technology products, which provide a comprehensive reference list for practitioners in the field; •The review within the study suggests only a limited number of research and design methods are regularly used by industrial design focused assistive technology new product developers; and, •Debate is required within the practitioners working in this field to reflect on how a wider range of potentially more effective methods and heuristics may be incorporated into daily working practice.

  17. Structural and optical study of CaF{sub 2} nanoparticles produced by a microwave-assisted hydrothermal method

    Energy Technology Data Exchange (ETDEWEB)

    Bezerra, Claudiane dos S., E-mail: cdsantos20@gmail.com; Valerio, Mário E.G.

    2016-11-15

    CaF{sub 2} nanoparticles were synthesized by the microwave-assisted hydrothermal method. With the addition of the ethylenediamine (EDA) as chelating agent, the size of the particles was reduced. The CaF{sub 2} exhibit single phase identified for X-ray diffraction (XRD) and confirmed by Rietveld refinement. Scanning electron microscopy (SEM) images showed nanoparticles with non-uniform morphology and statistical analysis of collections of particles reviewed that the EDA decreases both the average particle size and average aspect ratio of the particles. The chemical composition of the surface of the particles was investigated by X-ray Photoelectron Spectroscopy (XPS) and the results indicated the presence of reasonable amounts of hydroxyl groups and oxygen ions in the samples produced with EDA. Radioluminescence (RL) measurements showed that both types of nanoparticles presented intrinsic scintillation emission formed by two main bands and that the CaF{sub 2} samples produced without EDA presented higher emission intensity. The broad RL band centered at approximately 293 nm is related to self-trapped exciton (STE) emission of calcium fluoride, while the band at 428 nm can be due to the presence of F centers. The STE excitation and optical band gap were measured through photoluminescent excitation spectra in the VUV range.

  18. Chelating agent-free, vapor-assisted crystallization method to synthesize hierarchical microporous/mesoporous MIL-125 (Ti).

    Science.gov (United States)

    McNamara, Nicholas D; Hicks, Jason C

    2015-03-11

    Titanium-based microporous heterogeneous catalysts are widely studied but are often limited by the accessibility of reactants to active sites. Metal-organic frameworks (MOFs), such as MIL-125 (Ti), exhibit enhanced surface areas due to their high intrinsic microporosity, but the pore diameters of most microporous MOFs are often too small to allow for the diffusion of larger reactants (>7 Å) relevant to petroleum and biomass upgrading. In this work, hierarchical microporous MIL-125 exhibiting significantly enhanced interparticle mesoporosity was synthesized using a chelating-free, vapor-assisted crystallization method. The resulting hierarchical MOF was examined as an active catalyst for the oxidation of dibenzothiophene (DBT) with tert-butyl hydroperoxide and outperformed the solely microporous analogue. This was attributed to greater access of the substrate to surface active sites, as the pores in the microporous analogues were of inadequate size to accommodate DBT. Moreover, thiophene adsorption studies suggested the mesoporous MOF contained larger amounts of unsaturated metal sites that could enhance the observed catalytic activity.

  19. Perovskites of Type LaBO3 Prepared by the Microwave-Assisted Method for Oxygen Production

    Science.gov (United States)

    Ali, Shimaa M.; Atta, Nada F.; Al-Rahman, Yasser M. Abd; Galal, Ahmed

    LaBO3 (B = Ni, Mn, Cr and Co) were prepared by microwave-assisted citrate method. The electrocatalytic activity toward oxygen evolution reaction was investigated by dc linear polarization and electrochemical impedance measurements. XRD characterization showed that pure perovskite crystals were formed. FESEM images showed that changing the type of the B-site metal ion affected the morphology of the prepared perovskites. HRTEM images confirmed the formation of the orthorhombic phase LaCrO3 and the rhombohedral phases of LaNiO3, LaMnO3 and LaCoO3. The order of the electrocatalytic activity was LaNiO3 > LaFeO3 > LaMnO3, that was related to the calculated values of the activation energy 11.38, 20.13 and 22.01 kJ mol-1 for LaBO3 (B = Ni, Fe and Mn), respectively. LaCrO3 and LaCoO3 showed no catalytic activity toward OER due to the dissolution and the formation of passive layer of Co3O4 when being tested in HClO4 medium. The reaction order and mechanism were identified.

  20. Structural and optical characterization of CuInS2 quantum dots synthesized by microwave-assisted continuous flow methods

    Science.gov (United States)

    Fitzmorris, Robert C.; Oleksak, Richard P.; Zhou, Zheng; Mangum, Benjamin D.; Kurtin, Juanita N.; Herman, Gregory S.

    2015-07-01

    Semiconductor quantum dots (QDs) have recently been incorporated into consumer displays and lighting technologies. Now that these materials are being produced on industrial scales, it is important to investigate scalable synthetic methods and less toxic materials and chemistries. To achieve these goals, we have synthesized cadmium-free, visible light-emitting QDs using a microwave-assisted continuous flow reactor. After synthesis, the CuInS2 QD cores underwent a near-complete Zn cation exchange reaction in a batch reactor, followed by the growth of a ZnS shell. Analysis of X-ray diffraction, transmission electron microscopy, and Raman spectroscopy data indicate that the crystal structure changes from CuInS2 (chalcopyrite) to ZnS (zincblende) during the cation exchange reaction. Compositional analysis indicated that the core/shell QDs were 98 % ZnS, with Cu and In present at much lower concentrations. The photoluminescence (PL) peak position was blue shifted for longer cation exchange reactions, and it was found that the ZnS shell was necessary for improved PL stability. The synthesized QDs have a PL down conversion efficiency of 65 % when using a blue LED source.

  1. A SIMPLE AND RAPID MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHT MASS SPECTROMETRY METHOD TO SCREEN FISH PLASMA SAMPLES FOR ESTROGEN-RESPONSIVE BIOMARKERS

    Science.gov (United States)

    In this study, we describe and evaluate the performance of a simple and rapid mass spectral method for screening fish plasma for estrogen-responsive biomarkers using matrix assisted laster desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) couopled with a short...

  2. A Different Approach to Have Science and Technology Student-Teachers Gain Varied Methods in Laboratory Applications: A Sample of Computer Assisted POE Application

    Science.gov (United States)

    Saka, Arzu

    2012-01-01

    The purpose of this study is to develop a new approach and assess the application for the science and technology student-teachers to gain varied laboratory methods in science and technology teaching. It is also aimed to describe the computer-assisted POE application in the subject of "Photosynthesis-Light" developed in the context of…

  3. The Effectiveness of a Facebook-Assisted Teaching Method on Knowledge and Attitudes about Cervical Cancer Prevention and HPV Vaccination Intention among Female Adolescent Students in Taiwan

    Science.gov (United States)

    Lai, Ching-Yi; Wu, Wei-Wen; Tsai, Shao-Yu; Cheng, Su-Fen; Lin, Kuan-Chia; Liang, Shu-Yuan

    2015-01-01

    Background: Lack of education is a known barrier to vaccination, but data on the design and effectiveness of interventions remain limited. Objective: This study aims to identify the effectiveness of a Facebook-assisted teaching method on female adolescents' knowledge and attitudes about cervical cancer prevention and on their human papillomavirus…

  4. The Views of Pre-Service Teachers Regarding the Effectiveness of Peer Assisted Learning Method in the Science and Technology Laboratory Practices Course

    Science.gov (United States)

    Simsekli, Yeter; Özer, Dilek Zeren; Güngör, Sema Nur

    2017-01-01

    The purpose of this study is to show the views of pre-service teachers about peer-assisted learning method which is a common practice. The peer student group of the research sample (N:40) consisted of 2nd grade pre-service primary teachers attending the Uludag University Faculty of Education during the 2010-2011 academic year and taking the…

  5. The Effects of Computer-Assisted Instruction Based on Top-Level Structure Method in English Reading and Writing Abilities of Thai EFL Students

    Science.gov (United States)

    Jinajai, Nattapong; Rattanavich, Saowalak

    2015-01-01

    This research aims to study the development of ninth grade students' reading and writing abilities and interests in learning English taught through computer-assisted instruction (CAI) based on the top-level structure (TLS) method. An experimental group time series design was used, and the data was analyzed by multivariate analysis of variance…

  6. Treatment of anastomotic leakage after rectal resection with transrectal vacuum-assisted drainage (VAC). A method for rapid control of pelvic sepsis and healing

    DEFF Research Database (Denmark)

    Nagell, Carl Frederik Otto; Holte, Kathrine

    2006-01-01

    functional result is not uncommon. Vacuum-assisted closure (VAC) has been shown to accelerate wound healing by increasing local blood flow, reducing bacterial load and stimulating growth of granulation tissue. In this paper, we describe VAC as a method for treating anastomotic leakage after rectal resection...

  7. Avaliação da gordura orbitária de coelhos após enucleação e evisceração Orbital fat evaluation after enucleation and evisceration in rabbits

    Directory of Open Access Journals (Sweden)

    Claudia Akemi Shiratori

    2005-10-01

    Full Text Available OBJETIVO: Avaliar os adipócitos da gordura orbitária de coelhos após enucleação e evisceração. MÉTODOS: Foram estudados vinte e três espécimes de gordura orbitária, provenientes de 23 coelhos com idade de 42 dias, sendo 11 submetidos à cirurgia de enucleação e 12 à evisceração. Os animais foram sacrificados 30, 90 e 180 dias após a cirurgia, avaliando-se a gordura orbitária ao microscópio óptico (aumento de 200x e usando o programa IpWin 32. A área média celular foi calculada a partir do número de adipócitos por campo e da área de cada adipócito, tendo sido comparados os resultados dos grupos (enucleação e evisceração usando teste estatístico não paramétrico para avaliação da área dos adipócitos. RESULTADOS: Não houve diferença significativa entre a área média dos adipócitos quando considerado o procedimento cirúrgico (enucleação e evisceração, ou quando considerado o momento de sacrifício. CONCLUSÃO: Tendo em vista que a área dos adipócitos foi semelhante e não variou significativamente após a enucleação ou a evisceração, em período de observação de até 180 dias, a diminuição de volume orbitário que ocorre nas cavidades anoftálmicas deve ser conseqüência de outros mecanismos, como mudanças na distribuição espacial da gordura da órbita.PURPOSE: To evaluate rabbit adipocytes of the orbital fat tissue after enucleation and evisceration. METHODS: Twenty-tree specimens from 23 rabbits aged 42 days which had undergone socket surgery (evisceration and enucleation were evaluated. The animals were sacrificed on the 30th, 90th and 180th postoperative day. The orbital fat tissue was prepared for light microscope evaluation (magnification: 200x and analyzed by IpWin 32 software program. The number of cells in each field and the area of each adipocyte were used to calculate the cell median area. The data were compared between the groups (enucleation and evisceration and submitted to

  8. Management of Benign Prostatic Hyperplasia Larger than 100 ml: Simple Open Enucleation Versus Transurethral Laser Prostatectomy.

    Science.gov (United States)

    Elkoushy, Mohamed A; Elhilali, Mostafa M

    2016-06-01

    Benign prostatic hyperplasia (BPH) is one of the most common causes of lower urinary tract symptoms (LUTS) in aging men. Over the age of 60, more than a half of men have BPH and/or bothersome LUTS. Contemporary guidelines advocate surgery as the standard of care for symptomatic BPH after failure of medical therapy, where the choice of the appropriate surgical procedure depends on the prostate size. Transurethral resection of the prostate (TURP) and simple open prostatectomy (OP) have been considered for decades the reference-standard techniques for men with prostate smaller and larger than 80 ml, respectively. However, both procedures are potentially associated with considerable perioperative morbidity which prompted the introduction of a variety of minimally invasive surgical techniques with comparable long-term outcomes compared to TURP and OP. Nevertheless, the management of prostates larger than 100 ml remains a clinical challenge. Transurethral anatomical enucleation of the prostate utilizing different laser energy represents an excellent alternative concept in transurethral BPH surgery. These procedures gained popularity and demonstrated similar outcomes to OP with the advantages of favorable morbidity profiles and shorter catheter time and hospital stay. Despite the fact that OP remains a viable treatment option for patients with bothersome LUTS secondary to very large prostates, this procedure has been to a large extent replaced by these emerging enucleation techniques. Given the advent of surgical alternatives, the current review presents an evidence-based comparison of the efficacy and safety profile of the currently available transurethral laser techniques with the standard OP for the management of BPH due to adenomas larger than 100 ml.

  9. Diode laser (980 nm) enucleation of the prostate: a promising alternative to transurethral resection of the prostate.

    Science.gov (United States)

    Yang, Stephen S; Hsieh, Cheng-Hsing; Lee, Yi-Shin; Chang, Shang-Jen

    2013-02-01

    With good hemostatic ability, the end-firing continuous-wave diode laser at 980 nm was used to enucleate the prostate (DiLEP) for the treatment of benign prostatic obstruction (BPO). The study compared the patients' demographics and surgical outcomes between DiLEP and transurethral resection of the prostate (TURP). Patients with significant BPO and a total prostatic weight of 40 g or more who had undergone DiLEP (n = 74) or TURP (n = 52) during the same period at our hospital were enrolled for analysis. DiLEP was performed by a single surgeon (Yang), and TURP by three surgeons (Yang, Hsieh and Chang). The 4-U incision technique was developed for DiLEP. The diode laser ensured bloodless incision followed by blunt dissection using the resectoscope and laser fiber as an 'index finger' to enucleate the prostate. To prevent unexpected deep thermal damage, the power of the laser was set at 80 W and the laser beam was directed towards the bladder neck and not towards the prostatic capsule. Demographic data and perioperative parameters were comparable between the two groups, except that DiLEP resulted in a significantly lower drop in hemoglobin level (0.9 ± 1.0 vs. 1.6 ± 2.4 g/dl, p = 0.03), shorter catheterization time (41.2 ± 19.9 vs. 67.7 ± 33.3 h, p = 0.01), and shorter postoperative stay (2.9 ± 1.9 vs. 4.1 ± 6.2 days, p = 00.01). Delayed postoperative sloughing of necrotic tissue was not observed in the DiLEP group. Improvements in voiding parameters were comparable between the groups, and were sustained during a follow-up of up to 1 year. DiLEP provided better hemostasis than TURP as evidenced by less blood loss. The role of DiLEP treating BPO requires further investigation.

  10. Outcomes of transurethral resection and holmium laser enucleation in more than 60 g of prostate: A prospective randomized study.

    Science.gov (United States)

    Jhanwar, Ankur; Sinha, Rahul J; Bansal, Ankur; Prakash, Gaurav; Singh, Kawaljit; Singh, Vishwajeet

    2017-01-01

    Transurethral resection of prostate (TURP) is considered a gold standard surgical procedure. The management of benign prostatic hyperplasia (BPH) has undergone tremendous change in recent years and shifted from open to minimal invasive procedure. With the advancement in technology and skills of surgeons, lasers have been used more liberally, particularly holmium laser. Holmium laser enucleation of prostate (HoLEP) is seen as close rival of TURP. The objective if this study is to observe long- and short-term outcomes of transurethral resection and holmium laser enucleation in the prostate of more than 60 g. This prospective randomized study includes 164 patients. Inclusion criteria were age 60 g, gross hematuria secondary to BPH, recurrent urinary tract infection, acute urinary retention, postvoid residual >150 ml, and Schafer Grade II or more. BPH associated with neurogenic bladder, stricture urethra, and carcinoma prostate were excluded from the study. Group 1 comprises patients who underwent TURP and Group 2 comprises who underwent HoLEP. Follow-up was done at 1, 3, 6, 12, and 24 months after the surgery. Data of 144 patients were analyzed. The mean age of patients in TURP and HoLEP group was 66.78 ± 7.81 and 67.70 ± 7.44 years, respectively (P = 0.47), mean prostatic volume was 74.5 ± 12.56 and 75.6 ± 12.84 g, respectively (P = 0.60), operative time was 73.10 ± 10.49 and 89.56 ± 13.81 min, respectively (P = 0.0001). Mean resected tissue was 44.80 ± 9.87 and 48.49 ± 10.87, respectively (P = 0.03). The sexual function did not changed significantly in postoperative follow-up. HoLEP is associated with less blood loss, lower transfusion rates, and a shorter hospital stay. The disadvantage of HoLEP is longer operative time and postoperative dysuria.

  11. Holmium laser enucleation of the prostate: initial report of the first 230 Egyptian cases performed in a single center.

    Science.gov (United States)

    Abdel-Hakim, Amr M; Habib, Enmar I; El-Feel, Ahmed S; Elbaz, Ahmed G; Fayad, Amr M; Abdel-Hakim, Mahmoud A; Meshref, Alaa W

    2010-08-01

    To report our experience with the first 230 cases of holmium laser enucleation of the prostate (HoLEP) performed in a single center. A total of 230 cases of HoLEP were performed between June 2007 and June 2008. Mean age of patients was 69.8 +/- 10.3 years, and 21.3% of patients were either on anticoagulant or antiplatelet treatment. There was no limit for prostate size, with a mean prostate size of 86.5 +/- 65.4 g (range: 20-350 g). Follow-up was performed regularly at 1, 3, 6, and 12 months, assessing the Q(max), PVR, and International Prostate Symptom Score. Weight of prostate chips retrieved after morcellation was 78.6 +/- 61.3 g (range: 10-350), with enucleation time 102.2 +/- 55.4 minutes and morcellation time 19.3 +/- 10.1 minutes, leading an estimated efficiency rate of 0.64 g/min. The rate of decrease in prostate volume and prostate-specific antigen was 90.8% and 82.5%, respectively. At 1 month, mean Q(max) increased from 7.7 +/- 2.3 to 25.8 +/- 10.1 mL/s (P Kegel exercises, respectively. Only 1 patient with pancytopenia required blood transfusion and there was no case for transurethral resection syndrome. HoLEP is a safe and an effective modern modality for the treatment of symptomatic BPH regardless of the gland size, with satisfactory clinical outcome. Copyright 2010 Elsevier Inc. All rights reserved.

  12. Development and evaluation of microwave-assisted and ultrasound-assisted methods based on a quick, easy, cheap, effective, rugged, and safe sample preparation approach for the determination of bisphenol analogues in serum and sediments.

    Science.gov (United States)

    Song, Shanjun; Shao, Mingwu; Wang, Weihua; He, Yajuan; Dai, Xinhua; Wang, Huiyu; Liu, Liliang; Guo, Feng

    2017-12-01

    Microwave- and ultrasound-assisted methods based on a quick, easy, cheap, effective, rugged, and safe sample preparation approach followed by high-performance liquid chromatography with tandem mass spectrometry were developed for the simultaneous determination of eight bisphenol analogues in serum and sediment. The developed methods provided satisfactory extraction efficiency for the energy provided by microwaves and ultrasound. Compositions of commercial sorbents (primary secondary amine, MgSO4 , octadecyl-modified silica, and graphitized carbon black) were evaluated. The ultrasound-assisted method was suited for serum using primary secondary amine, MgSO4 , and octadecyl-modified silica as sorbents and a mixture of hexane and ethyl acetate as extraction solvent. The microwave-assisted method worked better for sediment with tetrahydrofuran and methanol as solvents and primary secondary amine, MgSO4 , octadecyl-modified silica, and graphitized carbon black as sorbents. Other experimental parameters, such as extraction temperature and time, were also optimized. The inter- and intraday relative standard deviations ranged from 2.7 to 5.5%. The limits of detection were between 0.1 and 1.0 ng/mL for serum and between 0.1 and 0.5 ng/g dry weight for sediment. The proposed methods were successfully applied to seven sediment and 20 human serum samples. The results showed that the developed methods were practical for the analysis and biomonitoring of bisphenols in sera and sediment. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. The Reliability of a Computer-Assisted Telephone Interview Version of the Ohio State University Traumatic Brain Injury Identification Method.

    Science.gov (United States)

    Cuthbert, Jeffrey P; Whiteneck, Gale G; Corrigan, John D; Bogner, Jennifer

    2016-01-01

    Provide test-retest reliability (>5 months) of the Ohio State University Traumatic Brain Injury Identification Method modified for use as a computer-assisted telephone interview (CATI) to capture traumatic brain injury (TBI) and other substantial bodily injuries among a representative sample of noninstitutionalized adults living in Colorado. Four subsamples of 50 individuals, including people with no major lifetime injury, a major lifetime injury but no TBI, TBI with no loss of consciousness, and TBI with loss of consciousness, were interviewed using the CATI Ohio State University Traumatic Brain Injury Identification Method between 6 and 18 months after an initial interview. Stratified random sample of Coloradans (n = 200) selected from a larger study of TBI. Cumulative, Severity and Age-related indices were assessed for long-term reliability. Cumulative indices were those that summed the total number of specific TBI severities across the lifetime; Severity indices included measures of the most severe type of injury incurred throughout the lifetime; and Age-related indices assessed the timing of specific injury types across the lifespan. Test-retest reliabilities ranged from poor to excellent. The indices demonstrating the greatest reliability were Severity measures, with intraclass correlations for ordinal indices ranging from 0.62 to 0.78 and Cohen κ ranging from 0.50 to 0.62. One Cumulative outcome demonstrated high reliability (0.70 for number of TBIs with loss of consciousness ≥30 minutes), while the remaining Cumulative outcomes demonstrated low reliability, ranging from 0.06 to 0.21. Age-related test-retest reliabilities were fair to poor, with intraclass correlations of 0.38 to 0.49 and Cohen κ of 0.32 and 0.34. The CATI-modified Ohio State University Traumatic Brain Injury Identification Method used in this study is an effective measure for evaluating the maximum TBI severity incurred throughout the lifetime within a general population survey. The

  14. Development of normal-suction boundary control method based on inflow cannula pressure waveform for the undulation pump ventricular assist device.

    Science.gov (United States)

    Ishii, Kohei; Saito, Itsuro; Isoyama, Takashi; Nakagawa, Hidemoto; Emiko, Nakano; Ono, Toshiya; Shi, Wei; Inoue, Yusuke; Abe, Yusuke

    2012-09-01

    It is desirable to obtain the maximum assist without suction in ventricular assist devices (VADs). However, high driving power of a VAD may cause severe ventricle suction that can induce arrhythmia, hemolysis, and pump damage. In this report, an appropriate VAD driving level that maximizes the assist effect without severe systolic suction was explored. The target driving level was set at the boundary between low driving power without suction and high driving power with frequent suction. In the boundary range, intermittent mild suction may occur. Driving power was regulated by the suction occurrence. The normal-suction boundary control method was evaluated in a female goat implanted with an undulation pump ventricular assist device (UPVAD). The UPVAD was driven in a semipulsatile mode with heartbeat synchronization control. Systolic driving power was adjusted using a normal-suction boundary control method developed for this study. We confirmed that driving power could be maintained in the boundary range. Occurrences of suction were evaluated using the suction ratio. We defined this ratio as the number of suction occurrences divided by the number of heartbeats. The suction ratio decreased by 70% when the normal-suction boundary control method was used. © 2012, Copyright the Authors. Artificial Organs © 2012, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

  15. Low temperature synthesis of nanocrystalline magnesium aluminate with high surface area by surfactant assisted precipitation method: Effect of preparation conditions

    Energy Technology Data Exchange (ETDEWEB)

    Mosayebi, Zeinab [Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan (Iran, Islamic Republic of); Rezaei, Mehran, E-mail: rezaei@kashanu.ac.ir [Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan (Iran, Islamic Republic of); Catalyst and Advanced Materials Research Laboratory, Chemical Engineering Department, Faculty of Engineering, University of Kashan, Kashan (Iran, Islamic Republic of); Hadian, Narges [Institute of Nanoscience and Nanotechnology, University of Kashan, Kashan (Iran, Islamic Republic of); Kordshuli, Fazlollah Zareie [Shiraz Petrochemical Co., Shiraz (Iran, Islamic Republic of); Meshkani, Fereshteh [Catalyst and Advanced Materials Research Laboratory, Chemical Engineering Department, Faculty of Engineering, University of Kashan, Kashan (Iran, Islamic Republic of)

    2012-09-15

    Highlights: ► MgAl{sub 2}O{sub 4} showed a high surface area and nanocrystalline structure. ► Addition of polymeric surfactant affected the structural properties of MgAl{sub 2}O{sub 4}. ► MgAl{sub 2}O{sub 4} prepared with surfactant showed a hollow cylindrical shape. -- Abstract: A surfactant assisted co-precipitation method was employed for the low temperature synthesis of magnesium aluminate spinel with nanocrystalline size and high specific surface area. Pluronic P123 triblock copolymer and ammonia solution were used as surfactant and precipitation agent, respectively. The prepared samples were characterized by thermal gravimetric and differential thermal gravimetric analyses (TG/DTG), X-ray diffraction (XRD), N{sub 2} adsorption (BET) and transmission electron microscopy (TEM) techniques. The effects of several process parameters such as refluxing temperature, refluxing time, pH, P123 to metals mole ratio (P123/metals) and calcination temperature on the structural properties of the samples were investigated. The obtained results showed that, among the process parameters pH and refluxing temperature have a significant effect on the structural properties of samples. The results revealed that increase in pH from 9.5 to 11 and refluxing temperature from 40 °C to 80 °C increased the specific surface area of prepared samples in the range of 157–188 m{sup 2} g{sup −1} and 162–184 m{sup 2} g{sup −1}, respectively. The XRD analysis showed the single-phase MgAl{sub 2}O{sub 4} was formed at 700 °C.

  16. Shape-control by microwave-assisted hydrothermal method for the synthesis of magnetite nanoparticles using organic additives

    Energy Technology Data Exchange (ETDEWEB)

    Rizzuti, Antonino [Politecnico di Bari, Dipartimento di Ingegneria Civile, Ambientale, del Territorio, Edile e di Chimica (Italy); Dassisti, Michele [Politecnico di Bari, Dipartimento di Meccanica, Management e Matematica (Italy); Mastrorilli, Piero, E-mail: p.mastrorilli@poliba.it [Politecnico di Bari, Dipartimento di Ingegneria Civile, Ambientale, del Territorio, Edile e di Chimica (Italy); Sportelli, Maria C.; Cioffi, Nicola; Picca, Rosaria A. [Università di Bari, Dipartimento di Chimica (Italy); Agostinelli, Elisabetta; Varvaro, Gaspare [Consiglio Nazionale delle Ricerche, Istituto di Struttura della Materia (Italy); Caliandro, Rocco [Consiglio Nazionale delle Ricerche, Istituto di Cristallografia (Italy)

    2015-10-15

    A simple and fast microwave-assisted hydrothermal method is proposed for the synthesis of magnetite nanoparticles. The addition of different surfactants (polyvinylpyrrolidone, oleic acid, or trisodium citrate) was studied to investigate the effect on size distribution, morphology, and functionalization of the magnetite nanoparticles. Microwave irradiation at 150 °C for 2 h of aqueous ferrous chloride and hydrazine without additives resulted in hexagonal magnetite nanoplatelets with a facet-to-facet distance of 116 nm and a thickness of 40 nm having a saturation magnetization of ∼65 Am{sup 2} kg{sup −1}. The use of polyvinylpyrrolidone led to hexagonal nanoparticles with a facet-to-facet distance of 120 nm and a thickness of 53 nm with a saturation magnetization of ∼54 Am{sup 2} kg{sup −1}. Additives such as oleic acid and trisodium citrate yielded quasi-spherical nanoparticles of 25 nm in size with a saturation magnetization of ∼70 Am{sup 2} kg{sup −1} and spheroidal nanoparticles of 60 nm in size with a saturation magnetization up to ∼82 Am{sup 2} kg{sup −1}, respectively. A kinetic control of the crystal growth is believed to be responsible for the hexagonal habit of the nanoparticles obtained without additive. Conversely, a thermodynamic control of the crystal growth, leading to spheroidal nanoparticles, seems to occur when additives which strongly interact with the nanoparticle surface are used. A thorough characterization of the materials was performed. Magnetic properties were investigated by Superconducting Quantum Interference Device and Vibrating Sample magnetometers. Based on the observed magnetic properties, the magnetite obtained using citrate appears to be a promising support for magnetically transportable catalysts.

  17. Controlling the size and morphology of griseofulvin nanoparticles using polymeric stabilizers by evaporation-assisted solvent-antisolvent interaction method

    Science.gov (United States)

    Kumar, Raj; Siril, Prem Felix

    2015-06-01

    Griseofulvin (GF) is a potential drug for cancer therapy. However, its application is limited by its poor water solubility. Ultrafine GF nanoparticles were prepared through evaporation-assisted solvent-antisolvent interaction method for improving its solubility. Acetone was used as the solvent and water was used as the antisolvent. It was observed that particle size could be controlled by varying the concentration of GF in acetone. Average particle size was very low, 16 ± 4 and 28 ± 8 nm, when the concentration of GF was 5 and 25 mM, respectively, in acetone. However, the particle size increased drastically to more than 3 µm, when the concentration was increased to 50 mM. Interestingly, the presence of optimized concentration of polyvinylpyrrolidone (PVP) and hydroxypropyl methylcellulose (HPMC) as stabilizers in the antisolvent resulted in significant reduction of particle size. Particle size decreased to less than 40 nm in the presence of the polymeric stabilizers, even when the concentration was 50 mM. Field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy imaging revealed that the polymeric stabilizers encapsulated very small GF particles and thus stabilized them. The solubility of GF-HPMC, GF-PVP, and the bare GF particles that were prepared from 50 mM solution (micro-GF) was nearly 24, 19, and 11 times, respectively, higher than that of raw-GF. In vitro dissolution studies revealed that almost 100 % of the drug was released in 60 min from GF-PVP and GF-HPMC. Fourier transform infrared spectroscopy did not detect any strong interaction between GF and the stabilizers. X-ray diffraction showed that the prepared GF nanoparticles and the micro-GF were in polymorphic form I. Differential scanning calorimetric studies showed that the crystallinity of the nanoformulated GF was only slightly lower than that of raw-GF. Thus, particle size reduction and the presence of stabilizers led to significant enhancement in

  18. A powerful method for transcriptional profiling of specific cell types in eukaryotes: laser-assisted microdissection and RNA sequencing.

    Directory of Open Access Journals (Sweden)

    Marc W Schmid

    Full Text Available The acquisition of distinct cell fates is central to the development of multicellular organisms and is largely mediated by gene expression patterns specific to individual cells and tissues. A spatially and temporally resolved analysis of gene expression facilitates the elucidation of transcriptional networks linked to cellular identity and function. We present an approach that allows cell type-specific transcriptional profiling of distinct target cells, which are rare and difficult to access, with unprecedented sensitivity and resolution. We combined laser-assisted microdissection (LAM, linear amplification starting from <1 ng of total RNA, and RNA-sequencing (RNA-Seq. As a model we used the central cell of the Arabidopsis thaliana female gametophyte, one of the female gametes harbored in the reproductive organs of the flower. We estimated the number of expressed genes to be more than twice the number reported previously in a study using LAM and ATH1 microarrays, and identified several classes of genes that were systematically underrepresented in the transcriptome measured with the ATH1 microarray. Among them are many genes that are likely to be important for developmental processes and specific cellular functions. In addition, we identified several intergenic regions, which are likely to be transcribed, and describe a considerable fraction of reads mapping to introns and regions flanking annotated loci, which may represent alternative transcript isoforms. Finally, we performed a de novo assembly of the transcriptome and show that the method is suitable for studying individual cell types of organisms lacking reference sequence information, demonstrating that this approach can be applied to most eukaryotic organisms.

  19. Evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry assisted, selective broth method to screen for vancomycin-resistant enterococci in patients at high risk.

    Science.gov (United States)

    Huang, Tsi-Shu; Lee, Susan Shin-Jung; Lee, Chia-Chien; Chen, Chiu-Yen; Chen, Fang-Chen; Chen, Bao-Chen; Sy, Cheng Len; Wu, Kuan-Sheng

    2017-01-01

    Bile esculin azide with vancomycin (BEAV) medium is a sensitive, but slightly less specific method for vancomycin-resistant enterococci (VRE) screening. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid method for identification of clinical pathogens. This study aimed to assess the performance of a novel combination screening test for VRE, using BEAV broth combined with MALDI-TOF MS. Clinical specimens were collected from patients at risk of VRE carriage, and tested by the novel combination method, using selective BEAV broth culture method followed by MALDI-TOF MS identification (SBEAVM). The reference method used for comparison was the ChromID VRE agar method. A total of 135 specimens were collected from 78 patients, and 63 specimens tested positive for VRE positive using the ChromID VRE method (positive rate 46.7%). The sensitivity, specificity, positive predictive value, and negative predictive value of SBEAVM method after an incubation period of 28 hours were 93.7%, 90.3%, 89.4%, and 94.2%, respectively. The SBEAVM method when compared to the ChromID VRE method had a shorter turnaround time (29 vs. 48-72 hours) and lower laboratory cost ($2.11 vs. $3.23 per test). This study demonstrates that SBEAVM is a rapid, inexpensive, and accurate method for use in VRE screening.

  20. [Mg/Al layered double hydroxides prepared by microwave-assisted co-precipitation method for the removal of bromate].

    Science.gov (United States)

    Zhong, Qiong; Li, Huan

    2014-04-01

    In this paper, Mg/Al layered double hydroxides (Mg/Al LDHs) were prepared by the microwave-assisted co-precipitation method and the conventional co-precipitation method. The samples were labeled as Mg/Al LDHs-MW and Mg/Al LDHs-H, respectively. Mg/Al LDHs were characterized by X-ray diffractometer (XRD), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR). The results showed that the application of microwave in the preparation process promoted the formation of smaller pore diameter and higher crystallinity particles. The pore size and particle size of Mg/Al LDHs-MW were 41.13 nm and 427.08 nm, respectively. Batch experiments were investigated to evaluate the effect of dosage, initial pH and regeneration frequencies for bromate removal. The conclusion showed that the process of bromate removal on Mg/Al LDHs could be described by the pseudo-second kinetic model. The Langmuir isotherm well described the experimental data, and the Mg/Al LDHs-MW has a stronger adsorption capacity while the maximum adsorption capacity (q(0)) of Mg/Al LDHs-MW for bromate was 321.26 microg x g(-1) which was larger than the q(0) (288.74 microg x g(-1)) of Mg/Al LDHs-H. For the continuous fixed-bed column, model simulations using the Thomas model showed that the experimental data obtained at three different columns packed with Mg/Al LDHs-MW were able to predict breakthrough curves. Simulating the maximum adsorption capacity of adsorption column for bromate removal was 288.81 microg x g(-1). When the bed depth was 10 cm, inlet concentration was 800 microg x L(-1) and flow rate was 4.0 mL x min, the correlation coefficient of model was 0.92, indicating that the experimental data was described well by the Thomas model.

  1. Methods for assisting recovery of damaged brain and spinal cord and treating various diseases using arrays of x-ray microplanar beams

    Science.gov (United States)

    Dilmanian, F Avraham [Yaphank, NY; Anchel, David J [Rocky Point, NY; Gaudette, Glenn [Holden, MA; Romanelli, Pantaleo [Monteroduni, IT; Hainfeld, James [Shoreham, NY

    2010-06-29

    A method of assisting recovery of an injury site of the central nervous system (CNS) or treating a disease includes providing a therapeutic dose of X-ray radiation to a target volume through an array of parallel microplanar beams. The dose to treat CNS injury temporarily removes regeneration inhibitors from the irradiated site. Substantially unirradiated cells surviving between beams migrate to the in-beam portion and assist recovery. The dose may be staggered in fractions over sessions using angle-variable intersecting microbeam arrays (AVIMA). Additional doses are administered by varying the orientation of the beams. The method is enhanced by injecting stem cells into the injury site. One array or the AVIMA method is applied to ablate selected cells in a target volume associated with disease for palliative or curative effect. Atrial fibrillation is treated by irradiating the atrial wall to destroy myocardial cells while continuously rotating the subject.

  2. TiO{sub 2} assisted photo-oxidation of wastewater prior to voltammetric determination of trace metals: Eco-friendly alternative to traditional digestion methods

    Energy Technology Data Exchange (ETDEWEB)

    Lejbt, Beata; Ospina-Alvarez, Natalia; Miecznikowski, Krzysztof, E-mail: kmiecz@chem.uw.edu.pl; Krasnodębska-Ostręga, Beata, E-mail: bekras@chem.uw.edu.pl

    2016-12-01

    Highlights: • Cheap and eco-friendly method using anatase-assisted photodecomposition to pre-treat wastewater samples prior to trace metal analysis. • Addition of polyvinylidene fluoride did not affect the efficiency of organic matter decomposition. • TiO{sub 2} assisted photo-oxidation procedure is equally effective than ICP MS determination after UV assisted digestion with H{sub 2}O{sub 2} addition. - Abstract: Voltammetry is a sensitive method for metal determination and one alternative to ICP MS, but its limitation is the influence of the organic matrix on the measurements. To avoid those interferences, wet digestion with H{sub 2}O{sub 2} accelerated with UV irradiation is applied and evaporation of excess of reagents is required. In this study, photolytic oxidation in quartz tubes with anatase was carried out, using as object of study wastewater samples with high amount of organic matter. Cadmium and lead determination was carried out in order to test metal recoveries. Lead recoveries reached c.a 85% for UV digestion with hydrogen peroxide, 98% for digestion with anatase immobilized with polyvinylidene fluoride (N-metylo-2-pirolidon) and c.a 103% for digestion with immobilized anatase (anatase in gum). The results obtained showed the usefulness of TiO{sub 2} layer as an oxidation medium. An easy, cheap and eco-friendly digestion method of surfactants without any reagent has been developed, with equal sensitivity but and the same detection limit of traditional digestion methods.

  3. Enhanced production of α-form indomethacin using the antisolvent crystallization method assisted by N2 fine bubbles

    Science.gov (United States)

    Matsumoto, Masakazu; Ohno, Masatoshi; Wada, Yoshinari; Sato, Toshiyuki; Okada, Masaki; Hiaki, Toshihiko

    2017-07-01

    In this study, using the gas-liquid interfaces near fine bubbles as new crystallization fields where nucleation proceeds dominantly, a crystallization technique to enhance the production of α-form Indomethacin (IMC) at a constant temperature was developed. IMC is reported to show a complicated polymorphism that consists of five true polymorphs and a wide range of solvate forms, which are collectively named the β-form. IMC was crystallized using the antisolvent method assisted by nitrogen (N2) fine bubbles. In the regions around the minute gas-liquid interfaces, local supersaturation increases because of the accumulation of IMC, which is caused by the negative electric charge on the fine-bubble surface. Hence, the rate of crystal nucleation increases, and the crystallization of metastable polymorph is enhanced. At a solution temperature of 298 K, a saturated IMC-EtOH solution and water as an antisolvent were mixed by two different addition orders, as follows: the IMC-EtOH/H2O system: water was quickly added into a saturated IMC-EtOH solution; the H2O/IMC-EtOH system: a saturated IMC-EtOH solution was rapidly added into water. While water was mixed with the saturated IMC-EtOH solution, N2 fine bubbles with an average diameter of 25 μm were continuously supplied to the mixed solution using a self-supporting bubble generator and IMC was crystallized within a crystallization time of 5 min. In both systems, the supersaturation ratio in the bulk solution (lnC0/CS) was varied in the range of 1.5-5.7 by controlling the addition volume of water or saturated IMC-EtOH solution. For comparison, antisolvent crystallization free of fine bubbles was performed. The results show that in both systems, N2 fine-bubble injection enhanced the production of metastable α-form at ln(C0/CS) below 3.5, and decreased the required ln(C0/CS) for the high-yield crystallization of α-form.

  4. Controlling the size and morphology of griseofulvin nanoparticles using polymeric stabilizers by evaporation-assisted solvent–antisolvent interaction method

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Raj, E-mail: rk7410@gmail.com; Siril, Prem Felix, E-mail: prem@iitmandi.ac.in [Indian Institute of Technology Mandi, School of Basic Sciences and Advanced Material Research Centre (India)

    2015-06-15

    Griseofulvin (GF) is a potential drug for cancer therapy. However, its application is limited by its poor water solubility. Ultrafine GF nanoparticles were prepared through evaporation-assisted solvent–antisolvent interaction method for improving its solubility. Acetone was used as the solvent and water was used as the antisolvent. It was observed that particle size could be controlled by varying the concentration of GF in acetone. Average particle size was very low, 16 ± 4 and 28 ± 8 nm, when the concentration of GF was 5 and 25 mM, respectively, in acetone. However, the particle size increased drastically to more than 3 µm, when the concentration was increased to 50 mM. Interestingly, the presence of optimized concentration of polyvinylpyrrolidone (PVP) and hydroxypropyl methylcellulose (HPMC) as stabilizers in the antisolvent resulted in significant reduction of particle size. Particle size decreased to less than 40 nm in the presence of the polymeric stabilizers, even when the concentration was 50 mM. Field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy imaging revealed that the polymeric stabilizers encapsulated very small GF particles and thus stabilized them. The solubility of GF-HPMC, GF-PVP, and the bare GF particles that were prepared from 50 mM solution (micro-GF) was nearly 24, 19, and 11 times, respectively, higher than that of raw-GF. In vitro dissolution studies revealed that almost 100 % of the drug was released in 60 min from GF-PVP and GF-HPMC. Fourier transform infrared spectroscopy did not detect any strong interaction between GF and the stabilizers. X-ray diffraction showed that the prepared GF nanoparticles and the micro-GF were in polymorphic form I. Differential scanning calorimetric studies showed that the crystallinity of the nanoformulated GF was only slightly lower than that of raw-GF. Thus, particle size reduction and the presence of stabilizers led to significant

  5. Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application

    Directory of Open Access Journals (Sweden)

    Mantosh Kumar Satapathy

    2017-06-01

    Full Text Available Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for cross-linking in fabrication of the desired gelatin-graphene oxide (gel-GO nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v containing 0.1∼1 wt% of high-functionality nano-graphene oxide (GO. Optimized plasma conditions (2,500 V and 8.7 mA for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 µm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasma-assisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.

  6. Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application.

    Science.gov (United States)

    Satapathy, Mantosh Kumar; Chiang, Wei-Hung; Chuang, Er-Yuan; Chen, Chih-Hwa; Liao, Jia-Liang; Huang, Huin-Ning

    2017-01-01

    Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for cross-linking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1∼1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 µm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasma-assisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.

  7. The Effectiveness of an Outdoor Adventure Program as a Training Method for Resident Assistants. A Thesis in Recreation and Parks.

    Science.gov (United States)

    Cook, Keith Vincent

    A 3-day outdoor adventure training program for an experimental group of Resident Assistants (RAs) at Pennsylvania State University was implemented to determine the effects of such a program on group cohesiveness and self-confidence. There were 60 subjects in the control group and 55 in the experimental group; 59% of the control group were male and…

  8. The Computer-Assisted Web Interview Method as Used in the National Study of ICT Use in Primary Healthcare in Poland – Reflections on a Case Study

    Directory of Open Access Journals (Sweden)

    Sowa Paweł

    2015-12-01

    Full Text Available The development and widespread use of ICT in society are reflected by the way research is designed and conducted. The Computer Assisted Web Interview method is becoming more attractive and is a frequently used method in health sciences. The National Study of ICT Use in Primary Healthcare in Poland was conducted using this method. The aim of this paper is to present the major advantages and disadvantages of web surveys. Technical aspects of methodology and important stages of the aforementioned study, as well as key elements for its procedure, are mentioned. The authors also provide reflections based on their analysis of this national study, conducted between January and April 2014.

  9. Fully-automated computer-assisted method of CT brain scan analysis for the measurement of cerebrospinal fluid spaces and brain absorption density

    Energy Technology Data Exchange (ETDEWEB)

    Baldy, R.E.; Brindley, G.S.; Jacobson, R.R.; Reveley, M.A.; Lishman, W.A.; Ewusi-Mensah, I.; Turner, S.W.

    1986-03-01

    Computer-assisted methods of CT brain scan analysis offer considerable advantages over visual inspection, particularly in research; and several semi-automated methods are currently available. A new computer-assisted program is presented which provides fully automated processing of CT brain scans, depending on ''anatomical knowledge'' of where cerebrospinal fluid (CSF)-containing spaces are likely to lie. After identifying these regions of interest quantitative estimates are then provided of CSF content in each slice in cisterns, ventricles, Sylvian fissure and interhemispheric fissure. Separate measures are also provided of mean brain density in each slice. These estimates can be summated to provide total ventricular and total brain volumes. The program shows a high correlation with measures derived from mechanical planimetry and visual grading procedures, also when tested against a phantom brain of known ventricular volume. The advantages and limitations of the present program are discussed.

  10. Ultrasound assisted dispersive liquid-liquid microextraction followed by injector port silylation: a novel method for rapid determination of quinine in urine by GC-MS.

    Science.gov (United States)

    Jain, Rajeev; Mudiam, Mohana Krishna Reddy; Ch, Ratnasekhar; Chauhan, Abhishek; Khan, Haider A; Murthy, Rc

    2013-09-01

    Silylation is a widely used derivatization method for the analysis of polar analytes by GC-MS. Ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) is an ecofriendly, rapid and simple microextraction method. For the first time, a novel approach has been developed and applied for the analysis of quinine in urine by combining UA-DLLME with injection port silylation. The LOD and LOQ were found to be 5.4 and 18 ng/ml. The intra- and inter-day precisions were less than 5 and 8%, respectively. Mean recoveries of quinine were found to be in the range of 87 to 96%. Ultrasound-assisted dispersive liquid-liquid microextraction is rapid, simple and consumes less reagent for the analysis of polar analytes such as quinine.

  11. Introducing holmium laser enucleation of the prostate alongside transurethral resection of the prostate improves outcomes of each procedure.

    Science.gov (United States)

    Wilson, N; Mikhail, M; Acher, P; Lodge, R; Young, A

    2013-07-01

    Holmium laser enucleation of the prostate (HoLEP) is recognised as an alternative to transurethral resection of the prostate (TURP). HoLEP has been demonstrated to be at least as effective as TURP with less morbidity but its introduction to practice has been limited in part by the learning curve of a novel procedure. This study examined the effects of introducing HoLEP alongside an established practice of TURP on early morbidity and length of hospital stay (LOS). A retrospective review of all patients who underwent HoLEP and TURP between April 2007 and July 2011 was undertaken. HoLEP was introduced in April 2008; patients undergoing TURP before this were considered as a historical control group. Data were collected concerning resection/enucleation weight, blood transfusions and LOS. Overall, 772 patients underwent HoLEP or TURP within the 52-month study period: 164 underwent TURP prior to the introduction of HoLEP (TURP-A), 425 had TURP after the introduction of HoLEP (TURP-B) and 183 underwent HoLEP. The mean removed weight was 24g (standard deviation [SD]: 21g) for TURP-A, 19g for TURP-B (SD: 16g) and 38g (SD: 32g) for HoLEP (p<0.005). Blood transfusion rates were 5.5%, 2.2% and 1.6% for the TURP-A, TURP-B and HoLEP groups respectively (p<0.05). For TURP-A patients, the mean LOS was 5.6 days (SD: 3.5 days, 95% confidence interval [CI]: 5.3-6.0 days). The mean LOS for TURP-B patients was 4.4 days (SD: 4.4 days, 95% CI: 4.2-4.8 days). HoLEP patients had a mean LOS of 3.0 days (SD: 3.0 days, 95% CI: 2.6-3.4 days). The introduction of HoLEP alongside TURP is associated with lower rates of blood transfusion and shorter LOS for all patients. This is likely to be due to the use of HoLEP rather than TURP in patients with larger prostates, who are more likely to have complications.

  12. [Comparison of the diode laser and the thulium laser in transurethral enucleation of the prostate for treatment of benign prostatic hyperplasia].

    Science.gov (United States)

    Zhang, Feng-bo; Shao, Qiang; Tian, Ye

    2013-08-18

    To compare the validity and safety of diode laser and thulium laser transurethral enucleation of the prostate in benign prostatic hyperplasia (BPH) patients. In our study, 63 BPH patients treated with transurethral enucleation of the prostate were divided randomly into 2 groups by diode laser (Di group) or thulium laser (Thu group) respectively. The operation time, bleeding volume, electrolyte, international prostatic symptomatic score (IPSS), post-voiding residual volume (PVR) and maximum urine flow rate (Qmax) were recorded and compared. No difference was found in the 2 groups in basic preoperative characteristics. Di group was superior to Thu group in mean operation time [(61.5±19.6) min vs. (71.4±16.5) min, P=0.026] notwithstanding little clinical sense. The mean time of removing catheter was 2.1 d and 2.3 d respectively. No difference in either electrolyte decrease or hemoglobin decrease [(5.0±1.1 g/L) vs. (4.4±0.9) g/L, P=0.32] peri-operation between the 2 groups were found and no transurethral resection (TUR) syndrome was encountered. Lower urinary tract symptom (LUTS) in both the groups were released the 1st and 3nd months post-operation effectively in IPSS, PVR and Qmax respectively and comparable (P>0.05). Both the Diode laser and the Thulium laser transurethral enucleation of the prostate released the LUTS effectively in BPH patients and comparable in the short-time follow-up. Further study about late complications related to enucleation is necessary.

  13. Discrete reduction patterns of parvalbumin and calbindin D-28k immunoreactivity in the dorsal lateral geniculate nucleus and the striate cortex of adult macaque monkeys after monocular enucleation.

    Science.gov (United States)

    Blümcke, I; Weruaga, E; Kasas, S; Hendrickson, A E; Celio, M R

    1994-01-01

    We analyzed the immunohistochemical distribution of the two calcium-binding proteins, parvalbumin (PV) and calbindin D-28k (CB), in the primary visual cortex and lateral dorsal geniculate nucleus (dLGN) of monocularly enucleated macaque monkeys (Macaca fascicularis and Macaca nemestrina) in order to determine how the expression of PV and CB is affected by functional inactivity. The monkeys survived 1-17 weeks after monocular enucleation. The distribution pattern of each of the proteins was examined immunocytochemically using monoclonal antibodies and compared with that of the metabolic marker cytochrome oxidase (CO). We recorded manually the number of immunostained neurons and estimated the concentration of immunoreactive staining product using a computerized image-acquisition system. Our results indicate a decrease of approximately 30% in the labeling of PV-immunoreactive (ir) neuropil particularly in those layers of denervated ocular-dominance columns receiving the geniculocortical input. There was no change in the number of PV-ir neurons in any compartment irrespective of the enucleation interval. For CB-ir, we found a 20% decrease in the neuropil labeling in layer 2/3 of the denervated ocular-dominance columns. In addition, a subset of pyramidal CB-ir neurons in layers 2 and 4B, which are weakly stained in control animals, showed decreased labeling. In the dLGN of enucleated animals, PV-ir and CB-ir were decreased only in the neuropil of the denervated layers. From these results, we conclude that cortical interneurons and geniculate projection neurons still express PV and CB in their cell bodies after disruption of the direct functional input from one eye. The only distinct decrease of PV and CB expression is seen in axon terminals from retinal ganglion cells in the dLGN, and in the axons and terminals of both geniculocortical projection cells and cortical interneurons in the cerebral cortex.

  14. Gel-aided sample preparation (GASP)?A simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells

    OpenAIRE

    Fischer, Roman; Benedikt M Kessler

    2015-01-01

    We describe a ?gel-assisted? proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of...

  15. Vision-Related Quality of Life and Appearance Concerns Are Associated with Anxiety and Depression after Eye Enucleation: A Cross-Sectional Study.

    Science.gov (United States)

    Ye, Juan; Lou, Lixia; Jin, Kai; Xu, Yufeng; Ye, Xin; Moss, Timothy; McBain, Hayley

    2015-01-01

    To investigate the association of demographic, clinical and psychosocial variables with levels of anxiety and depression in participants wearing an ocular prosthesis after eye enucleation. This cross-sectional study included 195 participants with an enucleated eye who were attending an ophthalmic clinic for prosthetic rehabilitation between July and November 2014. Demographic and clinical data, and self-reported feelings of shame, sadness and anger were collected. Participants also completed the National Eye Institute Visual Function Questionnaire, the Facial Appearance subscale of the Negative Physical Self Scale, and the Hospital Anxiety and Depression Scale. Regression models were used to identify the factors associated with anxiety and depression. The proportion of participants with clinical anxiety was 11.8% and clinical depression 13.8%. More anxiety and depression were associated with poorer vision-related quality of life and greater levels of appearance concerns. Younger age was related to greater levels of anxiety. Less educated participants and those feeling more angry about losing an eye are more prone to experience depression. Clinical variables were unrelated to anxiety or depression. Anxiety and depression are more prevalent in eye-enucleated patients than the general population, which brings up the issues of psychiatric support in these patients. Psychosocial rather than clinical characteristics were associated with anxiety and depression. Longitudinal studies need to be conducted to further elucidate the direction of causality before interventions to improve mood states are developed.

  16. Vision-Related Quality of Life and Appearance Concerns Are Associated with Anxiety and Depression after Eye Enucleation: A Cross-Sectional Study.

    Directory of Open Access Journals (Sweden)

    Juan Ye

    Full Text Available To investigate the association of demographic, clinical and psychosocial variables with levels of anxiety and depression in participants wearing an ocular prosthesis after eye enucleation.This cross-sectional study included 195 participants with an enucleated eye who were attending an ophthalmic clinic for prosthetic rehabilitation between July and November 2014. Demographic and clinical data, and self-reported feelings of shame, sadness and anger were collected. Participants also completed the National Eye Institute Visual Function Questionnaire, the Facial Appearance subscale of the Negative Physical Self Scale, and the Hospital Anxiety and Depression Scale. Regression models were used to identify the factors associated with anxiety and depression.The proportion of participants with clinical anxiety was 11.8% and clinical depression 13.8%. More anxiety and depression were associated with poorer vision-related quality of life and greater levels of appearance concerns. Younger age was related to greater levels of anxiety. Less educated participants and those feeling more angry about losing an eye are more prone to experience depression. Clinical variables were unrelated to anxiety or depression.Anxiety and depression are more prevalent in eye-enucleated patients than the general population, which brings up the issues of psychiatric support in these patients. Psychosocial rather than clinical characteristics were associated with anxiety and depression. Longitudinal studies need to be conducted to further elucidate the direction of causality before interventions to improve mood states are developed.

  17. Thulium laser enucleation of the prostate (ThuLEP): transurethral anatomical prostatectomy with laser support. Introduction of a novel technique for the treatment of benign prostatic obstruction.

    Science.gov (United States)

    Herrmann, Thomas R W; Bach, T; Imkamp, F; Georgiou, A; Burchardt, M; Oelke, M; Gross, A J

    2010-02-01

    Transurethral removal of prostatic tissue is the treatment choice for benign prostatic enlargement and benign prostatic obstruction. Urodynamic results are directly linked to the amount of removed tissue which, however, is directly associated with intra- and postoperative morbidity. Transurethral laser operations of the prostate offer the advantage of decreased bleeding complications and the possibility to treat patients with bleeding disorders or anticoagulative treatment. The aim of the article is to present a novel technique of complete transurethral removal of the transition zone (enucleation) with the support of the Thulium laser to combine complete anatomical enucleation and maximum urodynamic efficacy with minimal side-effects. We present five distinct surgical steps for transurethral complete removal of the transition zone of the prostate (Thulium laser enucleation of the prostate, ThuLEP). Surgical steps are presented in chronological order with the help of intraoperative pictures. Laser energy of 70-90 W is only used for the incision at the verumontanum and bladder neck for removal of the middle lobe, whereas laser energy of 30 W was only used for coagulation of small vessel crossing the surgical capsule towards the transition zone and bladder neck for dissection of the lateral lobes. The lobes themselves are liberated by blunt dissection. ThuLEP offers complete removal of the transition zone no matter what prostatic size. The techniques combine maximum efficacy with minimal side-effects. Clinical results comparing ThuLEP with open prostatectomy or transurethral resection are awaited.

  18. Radical nephrectomy performed by open, laparoscopy with or without hand-assistance or robotic methods by the same surgeon produces comparable perioperative results

    Directory of Open Access Journals (Sweden)

    Tanya Nazemi

    2006-02-01

    Full Text Available PURPOSE: Radical nephrectomy can be performed using open or laparoscopic (with or without hand assistance methods, and most recently using the da Vinci Surgical Robotic System. We evaluated the perioperative outcomes using a contemporary cohort of patients undergoing radical nephrectomy by one of the above 4 methods performed by the same surgeon. MATERIALS AND METHODS: The relevant clinical information on 57 consecutive patients undergoing radical nephrectomy from September 2000 until July 2004 by a single surgeon was entered in a Microsoft Access DatabaseTM and queried. Following appropriate statistical analysis, p values < 0.05 were considered significant. RESULTS: Of 57 patients, the open, robotic, laparoscopy with or without hand assistance radical nephrectomy were performed in 18, 6, 21, and 12 patients, respectively. The age, sex, body mass index (BMI, incidence of malignancy, specimen and tumor size, tumor stage, Fuhrman grade, hospital stay, change in postoperative creatinine, drop in hemoglobin, and perioperative complications were not significantly different between the methods. While the estimated median blood loss, postoperative narcotic use for pain control, and hospital stay were significantly higher in the open surgery method (p < 0.05, the median operative time was significantly shorter compared to the robotic method (p = 0.02. Operating room costs were significantly higher in the robotic and laparoscopic groups; however, there was no significant difference in total hospital costs between the 4 groups. CONCLUSIONS: The study demonstrates that radical nephrectomy can be safely performed either by open, robotic, or laparoscopic with or without hand assistance methods without significant difference in perioperative complication rates. A larger cohort and longer follow up are needed to validate our findings and establish oncological outcomes.

  19. Comparison of ultrasound-assisted extraction with conventional extraction methods of oil and polyphenols from grape (Vitis vinifera L.) seeds.

    Science.gov (United States)

    Da Porto, Carla; Porretto, Erica; Decorti, Deborha

    2013-07-01

    Ultrasound-assisted extraction (US) carried out at 20 KHz, 150 W for 30 min gave grape seed oil yield (14% w/w) similar to Soxhlet extraction (S) for 6 h. No significant differences for the major fatty acids was observed in oils extracted by S and US at 150 W. Instead, K232 and K268 of US- oils resulted lower than S-oil. From grape seeds differently defatted (S and US), polyphenols and their fractions were extracted by maceration for 12 h and by ultrasound-assisted extraction for 15 min. Sonication time was optimized after kinetics study on polyphenols extraction. Grape seed extracts obtained from seeds defatted by ultrasound (US) and then extracted by maceration resulted the highest in polyphenol concentration (105.20mg GAE/g flour) and antioxidant activity (109 Eq αToc/g flour). Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Completeness of assisted bathing in nursing homes related to dementia and bathing method: results from a secondary analysis of cluster-randomised trial data.

    Science.gov (United States)

    van Achterberg, Theo; van Gaal, Betsie G I; Geense, Wytske W; Verbeke, Geert; van der Vleuten, Carine; Schoonhoven, Lisette

    2016-06-01

    Bathing assistance is a core element of essential care in nursing homes, yet little is known for quality of assisted bathing or its determinants. To explore differences in completeness of assisted bathing in relation to bathing method and resident characteristics. Secondary analysis of a cluster randomised trial including 500 nursing home residents designed to compare traditional bathing methods for skin effects and cost-consequences; GlinicalTrials.gov ID [NCT01187732]. Logistic mixed modelling was used to relate resident characteristics and bathing method to bathing completeness. Bathing completeness was highly variable over wards. Apart from a large effect for ward, logistic mixed modelling indicated bathing was more often complete in case of washing without water (using disposable skin cleaning and caring materials; estimate 2.55, SE 0.17, P < 0.0001) and less often complete in residents with dementia (estimate -0.22, SE 0.08, P = 0.0040). Introduction of washing without water is likely to lead to more bathing completeness in nursing homes. However, inequity in care was also identified with a view to highly variable bathing completeness over wards and more incomplete bathing by care staff in residents with dementia. Monitoring the performance of assisted bathing in nursing homes is indicated for the identification of undesirable variation in essential care and poorly performing teams. The introduction of washing without water could serve the promotion of bathing completeness in nursing homes overall, but will not solve inequity issues for residents. © 2015 John Wiley & Sons Ltd.

  1. Microwave assisted synthesis for A2E and development of LC-ESI-MS method for quantification of ocular bisretinoids in human retina.

    Science.gov (United States)

    Kotnala, A; Senthilkumari, S; Halder, N; Kumar, A; Velpandian, T

    2017-11-26

    To develop a microwave assisted method for the rapid synthesis of A2E and also to develop a method to quantify N-retinylidene-N-retinylethanolamine(A2E), all-trans retinal dimer (ATRD), A2-glycerophospho ethanolamine (A2GPE), dihydropyridine phosphatidyl ethanolamine (A2DHPE) and monofuran A2E (MFA2E) in age matched retina. The development of microwave assisted synthesis of A2E, its purification and characterization for its utility in quantification in human retina. The semi-quantitative method development using LC-ESI-MS, LC-ESI-MS/MS and LC-APCI-MS/MS from pooled macula and peripheral retina for the bisretinoid analysis has been done. Maximum A2E conversion using microwave assisted process took place at 80°C for 45min with a yield of 55.01%. Highly sensitive and specific mass spectrometric method was developed using reverse phase C-18 separation with positive electrospray ionization and positive atmospheric phase chemical ionization of tandom mass spectrometry. A gradient mobile phase separation was achieved using water and methanol with 0.1% TFA. Multiple reaction monitoring acquisition for ESI and APCI was performed at ATRD m/z 551.2/522.2, A2GPE m/z 746.4/729.5, A2DHPEm/z 594.4/576.5, MFA2E m/z 608.2/591.2, A2E m/z 592.4/418.2. Method was validated using LC-ESI-SIM mode to determine selectivity, linearity, sensitivity, precision and accuracy. An attempt towards optimization of the synthetic procedure of A2E was made so as to reduce the lengthy reaction time without compromising the yield. Developed method was capable enough for the detection of low level of bisretinids in retina. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Postoperative Lower Urinary Tract Storage Symptoms: Does Prostate Enucleation Differ from Prostate Vaporization for Treatment of Symptomatic Benign Prostatic Hyperplasia?

    Science.gov (United States)

    Elkoushy, Mohamed A; Elshal, Ahmed M; Elhilali, Mostafa M

    2015-10-01

    To assess the degree of postoperative storage symptoms after GreenLight™ laser photoselective vaporization of the prostate (PVP) and Holmium laser enucleation of the prostate (HoLEP) for management of benign prostatic hyperplasia (BPH) and its predictors. A retrospective review was performed for patients who underwent HoLEP or PVP for non catheter-dependent patients with BPH. Patients were followed up at 1, 3, 6, and 12 months and then annually by International Prostate Symptoms Score (IPSS), quality of life index, peak flow rate, residual urine volume, and prostate-specific antigen (PSA) level. Moderate or severe storage symptoms were defined as IPSS storage subscore ≥ 9. Of 1673 laser procedures, a total of 1100 procedures met the inclusion criteria including 809 HoLEPs and 291 PVPs. The HoLEP group had significantly larger preoperative prostates and longer operative time. In the HoLEP group, postoperative IPSS was significantly better than in the PVP group at all follow-up points (P100 minutes, and lower percent of postoperative PSA level reduction significantly predicted less improvement of postoperative storage symptoms regardless of the laser procedure. Storage urinary symptoms significantly improved more after HoLEP compared with PVP, irrespective of the generation of GreenLight laser used. Recovery from bothersome storage urinary symptoms after prostate vaporization is time dependent, and baseline degree of storage symptoms, prolonged operative time, and lower percent of postoperative PSA level reduction negatively predicts postoperative improvement of storage symptoms regardless of the laser procedure.

  3. Optical and Morphological Properties of ZnO- and TiO2-Derived Nanostructures Synthesized via a Microwave-Assisted Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    Nosipho Moloto

    2012-01-01

    Full Text Available A microwave-assisted hydrothermal method was used to synthesize ZnO and TiO2 nanostructures. The experimental results show that the method resulted in crystalline monodispersed ZnO nanorods that have pointed tips with hexagonal crystal phase. TiO2 nanotubes were also formed with minimum bundles. The mechanism for the formation of the tubes was validated by HRTEM results. The optical properties of both ZnO and TiO2 nanostructures showed characteristics of strong quantum confinement regime. The photoluminescence spectrum of TiO2 nanotubes shows good improvement from previously reported data.

  4. Acceptance of an assistive robot in older adults: a mixed-method study of human–robot interaction over a 1-month period in the Living Lab setting

    Directory of Open Access Journals (Sweden)

    Wu YH

    2014-05-01

    Full Text Available Ya-Huei Wu,1,2 Jérémy Wrobel,1,2 Mélanie Cornuet,1,2 Hélène Kerhervé,1,2 Souad Damnée,1,2 Anne-Sophie Rigaud1,21Hôpital Broca, Assistance Publique – Hôpitaux de Paris, 2Research Team 4468, Faculté de Médecine, Université Paris Descartes, Paris, FranceBackground: There is growing interest in investigating acceptance of robots, which are increasingly being proposed as one form of assistive technology to support older adults, maintain their independence, and enhance their well-being. In the present study, we aimed to observe robot-acceptance in older adults, particularly subsequent to a 1-month direct experience with a robot.Subjects and methods: Six older adults with mild cognitive impairment (MCI and five cognitively intact healthy (CIH older adults were recruited. Participants interacted with an assistive robot in the Living Lab once a week for 4 weeks. After being shown how to use the robot, participants performed tasks to simulate robot use in everyday life. Mixed methods, comprising a robot-acceptance questionnaire, semistructured interviews, usability-performance measures, and a focus group, were used.Results: Both CIH and MCI subjects were able to learn how to use the robot. However, MCI subjects needed more time to perform tasks after a 1-week period of not using the robot. Both groups rated similarly on the robot-acceptance questionnaire. They showed low intention to use the robot, as well as negative attitudes toward and negative images of this device. They did not perceive it as useful in their daily life. However, they found it easy to use, amusing, and not threatening. In addition, social influence was perceived as powerful on robot adoption. Direct experience with the robot did not change the way the participants rated robots in their acceptance questionnaire. We identified several barriers to robot-acceptance, including older adults’ uneasiness with technology, feeling of stigmatization, and ethical

  5. Acceptance of an assistive robot in older adults: a mixed-method study of human–robot interaction over a 1-month period in the Living Lab setting

    Science.gov (United States)

    Wu, Ya-Huei; Wrobel, Jérémy; Cornuet, Mélanie; Kerhervé, Hélène; Damnée, Souad; Rigaud, Anne-Sophie

    2014-01-01

    Background There is growing interest in investigating acceptance of robots, which are increasingly being proposed as one form of assistive technology to support older adults, maintain their independence, and enhance their well-being. In the present study, we aimed to observe robot-acceptance in older adults, particularly subsequent to a 1-month direct experience with a robot. Subjects and methods Six older adults with mild cognitive impairment (MCI) and five cognitively intact healthy (CIH) older adults were recruited. Participants interacted with an assistive robot in the Living Lab once a week for 4 weeks. After being shown how to use the robot, participants performed tasks to simulate robot use in everyday life. Mixed methods, comprising a robot-acceptance questionnaire, semistructured interviews, usability-performance measures, and a focus group, were used. Results Both CIH and MCI subjects were able to learn how to use the robot. However, MCI subjects needed more time to perform tasks after a 1-week period of not using the robot. Both groups rated similarly on the robot-acceptance questionnaire. They showed low intention to use the robot, as well as negative attitudes toward and negative images of this device. They did not perceive it as useful in their daily life. However, they found it easy to use, amusing, and not threatening. In addition, social influence was perceived as powerful on robot adoption. Direct experience with the robot did not change the way the participants rated robots in their acceptance questionnaire. We identified several barriers to robot-acceptance, including older adults’ uneasiness with technology, feeling of stigmatization, and ethical/societal issues associated with robot use. Conclusion It is important to destigmatize images of assistive robots to facilitate their acceptance. Universal design aiming to increase the market for and production of products that are usable by everyone (to the greatest extent possible) might help to

  6. Transurethral bipolar plasmakinetic vapo-enucleation of the prostate: Is it safe for patients on chronic oral anticoagulants and/or platelet aggregation inhibitors?

    Directory of Open Access Journals (Sweden)

    Waleed El-Shaer

    2017-12-01

    Full Text Available Objectives: To assess the safety and efficacy of bipolar plasmakinetic enucleation and resection of the prostate (PKERP for the management of benign prostatic hyperplasia (BPH in patients on oral anticoagulant (OAC therapy and/or platelet aggregation inhibitors (PAIs. Patients and methods: In all, 91 patients were recruited and underwent PKERP whilst they were receiving PAIs (aspirin, 56 patients; clopidogrel, three; aspirin and clopidogrel, 11. In all, 15 patients were receiving an OAC drug perioperatively, whilst another six patients were on dual PAIs and OACs. The primary outcomes were the perioperative morbidity and mortality rates. The secondary outcomes were functional outcomes including maximum urinary flow rate (Qmax, International Prostate Symptoms Score (IPSS, and post-void residual urine volume (PVR. Results: The mean (SD age of the patients was 65 (5.9 years, preoperative adenoma volume was 80.9 (30.4 mL, and the operative time was 67 (23 min. No patient developed serious perioperative cardiovascular complications. The mean (SD duration of hospital stay was 1.79 (1 days and the postoperative catheterisation time was 1.14 (0.76 days. The mean (SD haemoglobin drop was 0.74 (0.61 g/dL, blood transfusion rate was 2.2%, and the clot retention rate was 2.2%. The mean (SD postoperative Qmax was 18.6 (4.37 mL/s as compared to 7.2 (3.2 mL/s preoperatively (P < 0.001, and the preoperative IPSS was reduced from 24.3 (6.1 to 5.7 (2.3 postoperatively (P < 0.05. Prostate volume measured by transrectal ultrasonography was significantly reduced from a mean (SD of 80.9 (30.4 mL preoperatively to 29.5 (10.6 mL postoperatively (P < 0.001. Conclusion: Minimally invasive PKERP may be considered as a safe and effective treatment option for managing patients with BPH receiving OAC/PAI drugs. Keywords: Anticoagulant, BPH, LUTS, PKERP

  7. Holmium laser enucleation versus transurethral resection in patients with benign prostate hyperplasia: an updated systematic review with meta-analysis and trial sequential analysis.

    Directory of Open Access Journals (Sweden)

    Sheng Li

    Full Text Available BACKGROUND: Holmium laser enucleation (HoLEP in surgical treatment of benign prostate hyperplasia (BPH potentially offers advantages over transurethral resection of the prostate (TURP. METHODS: Published randomized controlled trials (RCTs were identified from PubMed, EMBASE, Science Citation Index, and the Cochrane Library up to October 10, 2013 (updated on February 5, 2014. After methodological quality assessment and data extraction, meta-analysis was performed using STATA 12.0 and Trial Sequential Analysis (TSA 0.9 software. RESULTS: Fifteen studies including 8 RCTs involving 855 patients met the criteria. The results of meta-analysis showed that: a efficacy indicators: there was no significant difference in quality of life between the two groups (P>0.05, but compared with the TURP group, Qmax was better at 3 months and 12 months, PVR was less at 6, 12 months, and IPSS was lower at 12 months in the HoLEP, b safety indicators: compared with the TURP, HoLEP had less blood transfusion (RR 0.17, 95% CI 0.06 to 0.47, but there was no significant difference in early and late postoperative complications (P>0.05, and c perioperative indicators: HoLEP was associated with longer operation time (WMD 14.19 min, 95% CI 6.30 to 22.08 min, shorter catheterization time (WMD -19.97 h, 95% CI -24.24 to -15.70 h and hospital stay (WMD -25.25 h, 95% CI -29.81 to -20.68 h. CONCLUSIONS: In conventional meta-analyses, there is no clinically relevant difference in early and late postoperative complications between the two techniques, but HoLEP is preferable due to advantage in the curative effect, less blood transfusion rate, shorter catheterization duration time and hospital stay. However, trial sequential analysis does not allow us to draw any solid conclusion in overall clinical benefit comparison between the two approaches. Further large, well-designed, multicentre/international RCTs with long-term data and the comparison between the two approaches remain open.

  8. Microwave-assisted cyclizations promoted by polyphosphoric acid esters: a general method for 1-aryl-2-iminoazacycloalkanes

    Directory of Open Access Journals (Sweden)

    Jimena E. Díaz

    2016-09-01

    Full Text Available The first general procedure for the synthesis of 5 to 7-membered 1-aryl-2-iminoazacycloalkanes is presented, by microwave-assisted ring closure of ω-arylaminonitriles promoted by polyphosphoric acid (PPA esters. 1-Aryl-2-iminopyrrolidines were easily prepared from the acyclic precursors employing a chloroformic solution of ethyl polyphosphate (PPE. The use of trimethylsilyl polyphosphate (PPSE in solvent-free conditions allowed for the synthesis of 1-aryl-2-iminopiperidines and hitherto unreported 1-aryl-2-iminoazepanes. The cyclization reaction involves good to high yields and short reaction times, and represents a novel application of PPA esters in heterocyclic synthesis.

  9. [The research and application of pretreatment method for matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi].

    Science.gov (United States)

    Huang, Y F; Chang, Z; Bai, J; Zhu, M; Zhang, M X; Wang, M; Zhang, G; Li, X Y; Tong, Y G; Wang, J L; Lu, X X

    2017-08-08

    Objective: To establish and evaluate the feasibility of a pretreatment method for matrix-assisted laser desorption ionization-time of flight mass spectrometry identification of filamentous fungi developed by the laboratory. Methods: Three hundred and eighty strains of filamentous fungi from January 2014 to December 2016 were recovered and cultured on sabouraud dextrose agar (SDA) plate at 28 ℃ to mature state. Meanwhile, the fungi were cultured in liquid sabouraud medium with a vertical rotation method recommended by Bruker and a horizontal vibration method developed by the laboratory until adequate amount of colonies were observed. For the strains cultured with the three methods, protein was extracted with modified magnetic bead-based extraction method for mass spectrum identification. Results: For 380 fungi strains, it took 3-10 d to culture with SDA culture method, and the ratio of identification of the species and genus was 47% and 81%, respectively; it took 5-7 d to culture with vertical rotation method, and the ratio of identification of the species and genus was 76% and 94%, respectively; it took 1-2 d to culture with horizontal vibration method, and the ratio of identification of the species and genus was 96% and 99%, respectively. For the comparison between horizontal vibration method and SDA culture method comparison, the difference was statistically significant (χ(2)=39.026, P method and vertical rotation method recommended by Bruker, the difference was statistically significant(χ(2)=11.310, P method and modified magnetic bead-based extraction method developed by the laboratory is superior to the method recommended by Bruker and SDA culture method in terms of the identification capacity for filamentous fungi, which can be applied in clinic.

  10. Spectrophotometric analysis of the effectiveness of a novel in-office laser-assisted tooth bleaching method using Er,Cr:YSGG laser.

    Science.gov (United States)

    Dionysopoulos, Dimitrios; Strakas, Dimitrios; Tolidis, Kosmas; Tsitrou, Effrosyni; Koumpia, Effimia; Koliniotou-Koumpia, Eugenia

    2017-11-01

    The purpose of this in vitro study was to compare the effectiveness of a novel Er,Cr:YSGG laser-assisted in-office tooth bleaching method with a conventional method by spectrophotometric analysis of the tooth color change. Furthermore, the influence of the application time of the bleaching gel on the effectiveness of the methods and the maintenance of the results 7 days and 1 month after the treatments were also evaluated. Twenty-four bovine incisors were stained and randomly distributed into four groups. Group 1 specimens received an in-office bleaching treatment with 35% H2O2 for 2 × 15 min. Group 2 specimens received the same treatment but with extended application time (2 × 20 min). In Group 3, the same in-office bleaching procedure (2 × 15 min) was carried out as that in Group 1, using Er,Cr:YSGG laser irradiation for 2 × 15 s on each specimen to catalyze the reaction of H2O2 breakdown. Group 4 specimens received the same bleaching treatment as Group 3 but with extended application time (2 × 20 min). Er,Cr:YSGG laser-assisted tooth bleaching treatment is more effective than the conventional treatment regarding color change of the teeth. Application time of the bleaching agent may influence the effectiveness of the methods. The color change of the tested treatments decreases after 7 days and 1 month. The clinical relevance of this study is that this novel laser-assisted bleaching treatment may be more advantageous in color change and application time compared to the conventional bleaching treatment.

  11. Prepare core–multishell CdSe/ZnS nanocrystals with pure color and controlled emission by tri-n-octylphosphine-assisted method

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Cuiling, E-mail: rencl@lzu.edu.cn [College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000 (China); Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000 (China); Hao, Junjie, E-mail: haojj@sustc.edu.cn [Department of Electrical & Electronic Engineering, South University of Science and Technology of China, Shenzhen 518055 (China); Chen, Hongli [College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000 (China); Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000 (China); Wang, Kai, E-mail: wangk@sustc.edu.cn [Department of Electrical & Electronic Engineering, South University of Science and Technology of China, Shenzhen 518055 (China); Wu, Dan [School of Electrical and Electronic Engineering, Nanyang Technological University (Singapore)

    2015-10-30

    Graphical abstract: The core–multishell CdSe/ZnS QDs synthesized by the TOP-assisted SILAR method represent pure color, high luminescence and controlled emission wavelength, which can be continuously tuned by simply varying the emission of the core nanocrystals. - Highlights: • The prepared core–multishell QDs have pure color emission (FWHM, <25 nm) even after coating with 3-monolayer ZnS. • The emission wavelength can be continuously adjusted by simply varying the ODA:Cd ratio for preparing the core nanocrystals. • The CdSe/ZnS QDs still have good optical properties synthesized at 30 multi scales. • The knowledge gained in this study enabled us to better understand the mechanism of TOP-assisted method. - Abstract: Core–multishell semiconductor nanocrystals have great potential in light emitting devices (LEDs) display, fluorescent biomarkers and luminescent solar concentrators. However, their applications are strongly limited due to the wide full-width at half-maximum (FWHM), inaccurate controllable emission wavelength, and decreased quantum yield as the shell coverage growth. So there still remains a great challenge for improving the photoluminescence properties of core–multishell quantum dots. In this work, tri-n-octylphosphine (TOP) assisted method was used to prepare CdSe/ZnS QDs with narrow FWHM and controlled emission wavelength, the influence of experimental conditions on the photoluminescent properties of the core–multishell QDs were investigated. The experimental results indicated this is an effective method to prepare core–multishell QDs with pure color emission (FWHM value is smaller than 25 nm after coating with 3 monolayers of ZnS), accurately controlled emission and high QY (>95%). This is the smallest FWHM for core–multishell QDs. The emission wavelength of the as-prepared core–multishell QDs can be continuously tuned by simply varying the emission of the core nanocrystals. Furthermore, the knowledge gained in this study

  12. Development of a microprocessing-assisted cell-systematic evolution of ligands by exponential enrichment method for human umbilical vein endothelial cells

    Science.gov (United States)

    Terazono, Hideyuki; Kim, Hyonchol; Nomura, Fumimasa; Yasuda, Kenji

    2016-06-01

    We developed a microprocessing-assisted technique to select single-strand DNA aptamers that bind to unknown targets on the cell surface by modifying the conventional systematic evolution of ligands by exponential enrichment (cell-SELEX). Our technique involves 1) the specific selection of target-cell-surface-bound aptamers without leakage of intracellular components by trypsinization and 2) cloning of aptamers by microprocessing-assisted picking of single cells using magnetic beads. After cell-SELEX, the enriched aptamers were conjugated with magnetic beads. The aptamer-magnetic beads conjugates attached to target cells were collected individually by microassisted procedures using microneedles under a microscope. After that, the sequences of the collected magnetic-bead-bound aptamers were identified. As a result, a specific aptamer for the surface of target cells, e.g., human umbilical vein endothelial cells (HUVECs), was chosen and its specificity was examined using other cell types, e.g., HeLa cells. The results indicate that this microprocessing-assisted cell-SELEX method for identifying aptamers is applicable in biological research and clinical diagnostics.

  13. "Helping someone with a skill sharpens it in your own mind": a mixed method study exploring health professions students experiences of Peer Assisted Learning (PAL).

    Science.gov (United States)

    Carr, Sandra E; Brand, Gabrielle; Wei, Li; Wright, Helen; Nicol, Pam; Metcalfe, Helene; Saunders, Julie; Payne, John; Seubert, Liza; Foley, Laurie

    2016-02-04

    Peer assisted learning (PAL) has been described as "the development of knowledge and skill through active help and support among status equals or matched companions". To enhance the learning experience of health professions students and improve collaborative and collegial learning, six pilot Peer Assisted Learning (PAL) projects were conducted across a health science faculty. A responsive mixed method evaluation design was applied to explore the adequacy of the preparation for PAL, the impact PAL had on student attainment of examination, consultation, communication and feedback skills and to explore students' learning experiences through PAL. The 149 participants agreed the training programme was well organised, offered a safe learning environment and prepared the participant for the PAL activity. The impact of PAL included improvements in students' confidence and ability to give feedback and developed students' teaching, clinical and communication skills. Qualitative analysis revealed participants experienced deeper learning through teaching and learning from their peers, became more open to giving and receiving feedback and valued the comfortable/safe learning environment offered through PAL. Providing appropriate training in peer teaching and feedback and the schools engagement and openness to peer learning in the classroom and clinical setting enhances students' peer assisted learning experience.

  14. Laser enucleation of the prostate: Overview of our results after the ...

    African Journals Online (AJOL)

    Y. Kharbach

    2017-08-01

    Aug 1, 2017 ... prostate hyperplasia for many decades [4]. Despite the good treat- ment results, there is a significant risk of complications for both techniques, thus explaining the unceasing efforts in researching alternative therapeutic options such as laser therapy methods [5]. It is clear that Holmium laser treatments can ...

  15. Evaluation of ultrasound velocity in enucleated equine aqueous humor, lens and vitreous body

    NARCIS (Netherlands)

    Meister, Ulrike; Ohnesorge, Bernhard; Körner, Daniel; Boevé, Michael H

    2014-01-01

    BackgroundSonographic ophthalmic examinations have become increasingly important in veterinary medicine. If the velocity of ultrasound in ocular tissues is known, the A-mode ultrasound method may be used to determine the axial intraocular distances, such as anterior chamber depth, lens thickness,

  16. Microwave-assisted extraction and a new determination method for total steroid saponins from Dioscorea zingiberensis C.H. Wright.

    Science.gov (United States)

    Ren, Yao; Chen, Yu; Hu, Bohan; Wu, Hui; Lai, Furao; Li, Xiaofeng

    2015-12-01

    An efficient microwave-assisted extraction (MAE) technique was applied to isolate total steroid saponins from Dioscorea zingiberensis C.H. Wright (DZW). The optimal extracting conditions were established as 75% ethanol as solvent, ratio of solid/liquid 1:20 (g/ml), temperature 75 °C, irradiation power 600 W and three extraction cycles of 6 min each. Scanning electron microscopy (SEM) images of DZW processed by four different extractions provided visual evidence of the disruption effect on DZW. Diosgenin was quantified by HPLC and examined further by LC-ESI/MS after acid hydrolysis. Total steroid saponins were calculated using diosgenin from total steroid saponins. The MAE procedure was optimized, validated and compared with other conventional extraction processes. This report provides a convenient technology for the extraction and quantification of total saponins of DZW combining MAE with HPLC and LC-ESI/MS for the first time. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Visible light-induced photocatalytic activity of Bi2O3 prepared via microwave-assisted method.

    Science.gov (United States)

    Liu, Xinjuan; Pan, Likun; Li, Jinliang; Yu, Kai; Sun, Zhuo

    2013-07-01

    Bi2O3 was successfully synthesized via microwave-assisted reaction of Bi2O3 precursor in aqueous solution using a microwave system. Their morphologies, structures and photocatalytic performances in the degradation of methylene blue (MB) were characterized by scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction spectroscopy, photoluminescence spectroscopy and UV-vis absorption spectroscopy, respectively. The results show that Bi2O3 synthesized at pH value of 7 exhibits an optimal photocatalytic performance with the MB degradation rate of 76% at 240 min under visible light irradiation due to its higher visible light absorption and comparatively low electron-hole pair recombination.

  18. Assistance at mealtimes in hospital settings and rehabilitation units for older adults from the perspective of patients, families and healthcare professionals: a mixed methods systematic review protocol.

    Science.gov (United States)

    Edwards, Deborah; Carrier, Judith; Hopkinson, Jane

    2015-11-01

    The review question is: assistance at mealtimes for older adults in hospital settings and rehabilitation units: what goes on, what works and what do patients, families and healthcare professionals think about it?The specific objectives are:This mixed methods review seeks to develop an aggregated synthesis of quantitative and qualitative data on assistance at mealtimes for older adults in hospital settings and rehabilitation units in order to derive conclusions and recommendations useful for clinical practice and policy decision making. Worldwide, it is estimated that between 20% and 50% of all adult patients admitted to hospital wards are malnourished. Reported prevalence occurs, depending on the specific patient group of interest, type of healthcare setting, disease state and criteria used to assess malnutrition. For older adults in hospital (over 65 years) the prevalence of malnutrition has been reported as being as high as 60% and can continue to deteriorate during the hospital stay. This is an area of concern as it is associated with prolonged hospital stays and increased morbidity (pressure ulcers, infections and falls) and mortality, especially for those with chronic conditions.Malnutrition in adults in developed countries is frequently associated with disease and may occur because of reduced dietary intake, malabsorption, increased nutrient losses or altered metabolic demands, with reduced dietary intake being considered the single most important aetiological factor. For the hospitalized older adult patient with pre-existing malnutrition, further nutritional problems are often encountered due to a reduced dietary intake. Poor food intake for older patients in hospital may be due to the effects of acute illness, poor appetite, nausea or vomiting, "nil by mouth" orders, medication side effects, catering limitations, swallowing and/or oral problems, difficulty with vision and opening containers, the placement of food out of the patients' reach, limited access

  19. Effect of two recovery methods on repeated closed-handed and open-handed weight-assisted pull-ups.

    Science.gov (United States)

    Bacon, Nicholas T; Wingo, Jonathan E; Richardson, Mark T; Ryan, Greg A; Pangallo, Tracey C; Bishop, Phillip A

    2012-05-01

    The purpose of this study was to examine 2 recovery modalities (with and without an ice bag treatment) on closed-handed and open-handed weight-assisted pull-ups in recreationally-trained rock climbers. Healthy and recreationally active volunteers (n = 9) completed 4 counterbalanced trials separated by 72 hours. Trials included 3 sets of closed-handed and open-handed weight-assisted pull-ups supported by 50% of body weight, until failure. Between each set, participants sat quietly in a chair and engaged in approximately 20 minutes of either passive or ice bag treatment. Ice bags were placed on the participants' arms and shoulders. Heart rate (HR), ratings of perceived exertion (RPE), session-RPE (S-RPE), and perceived recovery were also assessed. Hand-grip strength pretrial and posttrial was not different between ice bag conditions. Also, there were no differences between treatments for HR, RPE, perceived recovery, S-RPE, or comfort ratings. The overall number of open-handed pull-ups (mean ± SD = 19 ± 5) was lower than closed-handed pull-ups (34 ± 14; p up performance for sets 2 (22 ± 5; p = 0.004) and 3 (22 ± 5; p = 0.003) relative to set 3 using passive recovery only (i.e., no ice bag; 17 ± 6). There were no differences (p = 0.31) between treatments for closed-handed pull-ups. The findings support the recommendations to use ice bags for recovery between bouts of rock climbing that involve a predominantly open-handed grip to maintain performance.

  20. Comparison of Surgical Outcomes Between Holmium Laser Enucleation and Transurethral Resection of the Prostate in Patients With Detrusor Underactivity.

    Science.gov (United States)

    Woo, Myeong Jin; Ha, Yun-Sok; Lee, Jun Nyung; Kim, Bum Soo; Kim, Hyun Tae; Kim, Tae-Hwan; Yoo, Eun Sang

    2017-03-24

    Currently, holmium laser enucleation of the prostate (HoLEP) and transurethral resection of the prostate (TURP) are the standard surgical procedures used to treat benign prostatic hyperplasia (BPH). Several recent studies have demonstrated that the surgical management of BPH in patients with detrusor underactivity (DU) can effectively improve voiding symptoms, but comparative data on the efficacy of HoLEP and TURP are insufficient. Therefore, we compared the short-term surgical outcomes of HoLEP and TURP in patients with DU. From January 2010 to May 2015, 352 patients underwent HoLEP or TURP in procedures performed by a single surgeon. Of these patients, 56 patients with both BPH and DU were enrolled in this study (HoLEP, n=24; TURP, n=32). Surgical outcomes were retrospectively compared between the 2 groups. DU was defined as a detrusor pressure at maximal flow rate of <40 cm H2O as measured by a pressure flow study. The preoperative characteristics of patients and the presence of comorbidities were comparable between the 2 groups. The TURP group showed a significantly shorter operative time than the HoLEP group (P=0.033). The weight of the resected prostate was greater in the HoLEP group, and postoperative voiding parameters, including peak flow rate and postvoid residual urine volume were significantly better in the HoLEP group than in the TURP group. HoLEP can be effectively and safely performed in patients with DU and can be expected to have better surgical outcomes than TURP in terms of the improvement in lower urinary tract symptoms.

  1. Holmium laser enucleation versus transurethral resection of the prostate: 3-year follow-up results of a randomized clinical trial.

    Science.gov (United States)

    Ahyai, Sascha A; Lehrich, Karin; Kuntz, Rainer M

    2007-11-01

    To report 3-yr follow-up results of a randomised clinical trial comparing holmium laser enucleation of the prostate (HoLEP) with transurethral resection of the prostate (TURP). A total of 200 patients with urodynamic obstruction and a prostate volume of less than 100 cc were prospectively randomised and assigned to HoLEP or TURP. All patients were assessed preoperatively and followed at 1, 6, 12, 18, 24, and 36 mo postoperatively. American Urological Association Symptom Score (AUA SS), maximum flow rate (Q(max)), and postvoid residual (PVR) [urine] volume were obtained at each follow-up. Perioperative data and postoperative outcome were compared. All complications were recorded. AUA SS were significantly better 2 yr postoperatively in the HoLEP group (1.7 vs. 3.9, p<0.0001) and similar at 3 yr (2.7 vs. 3.3, p=0.17). PVR volume was significantly better 2 yr (5.6 vs. 19.9 ml, p<0.001) and 3 yr (8.4 vs. 20.2 ml, p=0.012) postoperatively in HoLEP patients. Q(max) was similar in the HoLEP and TURP groups at 2 yr (28.0 vs. 29.1 ml/s, p=0.83) and at 3 yr (29.0 vs. 27.5 ml/s, p=0.41) postoperatively. Late complications consisted of urethral strictures, bladder-neck contractures, and BPH recurrence; reoperation rates were 7.2% in the HoLEP and 6.6% in the TURP group (p=1.0). After 2 and 3 yr of follow-up, HoLEP micturition outcomes compare favourably with TURP. Late complications are equally low. HoLEP may be a real alternative to TURP.

  2. Comparison of Photoselective Vaporization versus Holmium Laser Enucleation for Treatment of Benign Prostate Hyperplasia in a Small Prostate Volume.

    Science.gov (United States)

    Kim, Kang Sup; Choi, Jin Bong; Bae, Woong Jin; Kim, Su Jin; Cho, Hyuk Jin; Hong, Sung-Hoo; Lee, Ji Youl; Kim, Sang Hoon; Kim, Hyun Woo; Cho, Su Yeon; Kim, Sae Woong

    2016-01-01

    Photoselective vaporization of the prostate (PVP) using GreenLight and Holmium laser enucleation of the prostate (HoLEP) is an important surgical technique for management of benign prostate hyperplasia (BPH). We aimed to compare the effectiveness and safety of PVP using a 120 W GreenLight laser with HoLEP in a small prostate volume. Patients who underwent PVP or HoLEP surgery for BPH at our institutions were reviewed from May 2009 to December 2014 in this retrospective study. Among them, patients with prostate volumes < 40 mL based on preoperative trans-rectal ultrasonography were included in this study. Peri-operative and post-operative parameters-such as International Prostate Symptom Score (IPSS), quality of life (QoL), maximum urinary flow rate (Qmax), post-void residual urine volume (PVR), and complications-were compared between the groups. PVP was performed in 176 patients and HoLEP in162 patients. Preoperative demographic data were similar in both groups, with the exception of PVR. Operative time and catheter duration did not show significant difference. Significant improvements compared to preoperative values were verified at the postoperative evaluation in both groups in terms of IPSS, QoL, Qmax, and PVR. Comparison of the postoperative parameters between the PVP and HoLEP groups demonstrated no significant difference, with the exception of IPSS voiding subscore at 1 month postoperatively (5.9 vs. 3.8, P< 0.001). There was no significant difference in postoperative complications between the two groups. Our data suggest that PVP and HoLEP are efficient and safe surgical treatment options for patients with small prostate volume.

  3. Comparison of Photoselective Vaporization versus Holmium Laser Enucleation for Treatment of Benign Prostate Hyperplasia in a Small Prostate Volume.

    Directory of Open Access Journals (Sweden)

    Kang Sup Kim

    Full Text Available Photoselective vaporization of the prostate (PVP using GreenLight and Holmium laser enucleation of the prostate (HoLEP is an important surgical technique for management of benign prostate hyperplasia (BPH. We aimed to compare the effectiveness and safety of PVP using a 120 W GreenLight laser with HoLEP in a small prostate volume.Patients who underwent PVP or HoLEP surgery for BPH at our institutions were reviewed from May 2009 to December 2014 in this retrospective study. Among them, patients with prostate volumes < 40 mL based on preoperative trans-rectal ultrasonography were included in this study. Peri-operative and post-operative parameters-such as International Prostate Symptom Score (IPSS, quality of life (QoL, maximum urinary flow rate (Qmax, post-void residual urine volume (PVR, and complications-were compared between the groups.PVP was performed in 176 patients and HoLEP in162 patients. Preoperative demographic data were similar in both groups, with the exception of PVR. Operative time and catheter duration did not show significant difference. Significant improvements compared to preoperative values were verified at the postoperative evaluation in both groups in terms of IPSS, QoL, Qmax, and PVR. Comparison of the postoperative parameters between the PVP and HoLEP groups demonstrated no significant difference, with the exception of IPSS voiding subscore at 1 month postoperatively (5.9 vs. 3.8, P< 0.001. There was no significant difference in postoperative complications between the two groups.Our data suggest that PVP and HoLEP are efficient and safe surgical treatment options for patients with small prostate volume.

  4. Efficient sample preparation method based on solvent-assisted dispersive solid-phase extraction for the trace detection of butachlor in urine and waste water samples.

    Science.gov (United States)

    Aladaghlo, Zolfaghar; Fakhari, Alireza; Behbahani, Mohammad

    2016-10-01

    In this work, an efficient sample preparation method termed solvent-assisted dispersive solid-phase extraction was applied. The used sample preparation method was based on the dispersion of the sorbent (benzophenone) into the aqueous sample to maximize the interaction surface. In this approach, the dispersion of the sorbent at a very low milligram level was achieved by inserting a solution of the sorbent and disperser solvent into the aqueous sample. The cloudy solution created from the dispersion of the sorbent in the bulk aqueous sample. After pre-concentration of the butachlor, the cloudy solution was centrifuged and butachlor in the sediment phase dissolved in ethanol and determined by gas chromatography with flame ionization detection. Under the optimized conditions (solution pH = 7.0, sorbent: benzophenone, 2%, disperser solvent: ethanol, 500 μL, centrifuged at 4000 rpm for 3 min), the method detection limit for butachlor was 2, 3 and 3 μg/L for distilled water, waste water, and urine sample, respectively. Furthermore, the preconcentration factor was 198.8, 175.0, and 174.2 in distilled water, waste water, and urine sample, respectively. Solvent-assisted dispersive solid-phase extraction was successfully used for the trace monitoring of butachlor in urine and waste water samples. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Feasibility of holmium laser enucleation of the prostate (HoLEP) for recurrent/residual benign prostatic hyperplasia (BPH).

    Science.gov (United States)

    Elshal, Ahmed M; Elmansy, Hazem M; Elhilali, Mostafa M

    2012-12-01

    Study Type--Therapy (case series) Level of Evidence 4. What's known on the subject? and What does the study add? The major advantage of holmium laser enucleation of the prostate (HoLEP) depends on the ability to use the native anatomical plane between the prostate adenoma and surgical capsule, peeling each prostatic lobe from the capsule. HoLEP is associated with less catheterisation time, hospital stay and blood loss than transurethral resection of the prostate (TURP) or open prostatectomy. Urodynamic relief of obstruction has been reported to be better with HoLEP than TURP. However, surgical treatment of recurrent prostatic obstruction after previous transurethral surgery for symptomatic benign prostatic hyperplasia is more challenging because of loss of anatomical landmarks resulting in either incomplete removal or incontinence. HoLEP for recurrent symptoms due to residual or re-growing prostatic adenoma seems to be as safe, feasible and efficient as HoLEP for de novo cases. The surgical plane between the adenoma and the surgical capsule was still accessible resulting in a durable long-term outcome with minimal side-effects. Previous transurethral prostatic surgery is not a contraindication for HoLEP. • To assess the technical feasibility, functional outcome and morbidity of holmium laser enucleation of the prostate (HoLEP) for symptomatic benign prostatic hyperplasia (BPH) in patients with previous transurethral prostate surgery. 'Redo' surgery for recurrent or residual BPH poses a technical challenge with uncertain outcome as a result of disturbed anatomical landmarks with no clear surgical limits. • We retrospectively reviewed 1054 patients who underwent HoLEP for symptomatic BPH. • Patients were stratified into two groups, group-I with no previous prostate surgery or primary-HoLEP (978 patients) and group-II with history of previous prostate surgery or secondary-HoLEP (76). • All patients' variables as well as follow-up data were assessed and

  6. Dental Assistants

    Science.gov (United States)

    ... State & Area Data Explore resources for employment and wages by state and area for dental assistants. Similar Occupations Compare the job duties, education, job growth, and pay of dental assistants with ...

  7. Assistive Technology

    Science.gov (United States)

    ... Page Resize Text Printer Friendly Online Chat Assistive Technology Assistive technology (AT) is any service or tool that helps ... be difficult or impossible. For older adults, such technology may be a walker to improve mobility or ...

  8. Low Energy Consumption Synthesis of Nanostructured TiO2 Particles by Combining Oxidant Peroxide Method and Microwave-Assisted Hydrothermal Treatment

    Directory of Open Access Journals (Sweden)

    A. P. Garcia

    2016-01-01

    Full Text Available Titanium dioxide with high specific surface area in the crystalline anatase phase is a promising material for environmental applications. In this work, TiO2 with good applicability for photocatalytic processes has been obtained using the low energy consumption synthesis based on oxidant peroxide method combined with microwave-assisted low temperature hydrothermal treatment. To prepare the material, titanium propoxide, hydrogen peroxide, and isopropyl alcohol were used. The influence of time and temperature during the hydrothermal step on properties like morphology, crystallinity, phase composition, specific surface area, and photocatalytic behavior were investigated. Photoactivity was measured using the methyl orange decomposition method in UV-A light. Increasing temperature during hydrothermal step, photocatalytic properties could be improved. The nanostructured TiO2 particles synthesized at 200°C and 30 min with this method showed photocatalytic activity comparable to commercial Aeroxide® TiO2 P25.

  9. Topographic measurements of eyelids and orbit in enucleated eyes with hydroxyapatite integrated implant versus PMMA implant

    OpenAIRE

    Gradinaru, S; Totir, M; Iancu, R; Leasu, C; Pricopie, S; Yasin, S; Ciuluvica, R; Ungureanu, E

    2014-01-01

    Introduction: This study reports our results relating to palpebral eyelid fissure and orbital measurements following evisceration with orbital implantation of hydroxyapatite integrated implant and PMMA implant. Materials and Methods: This study is a prospective study of 43 patients that underwent evisceration for different ocular affections at University Emergency Hospital Bucharest, Ophthalmology department between January 2009 and September 2010 (Group A comprising of twenty patients had th...

  10. Assistive Technologies

    Science.gov (United States)

    Auat Cheein, Fernando A., Ed.

    2012-01-01

    This book offers the reader new achievements within the Assistive Technology field made by worldwide experts, covering aspects such as assistive technology focused on teaching and education, mobility, communication and social interactivity, among others. Each chapter included in this book covers one particular aspect of Assistive Technology that…

  11. Automutilação ocular: relato de seis casos de enucleação ocular Self-mutilation: report of six cases of enucleation

    Directory of Open Access Journals (Sweden)

    Marcelo G Nucci

    2000-06-01

    Full Text Available No presente trabalho são relatados seis casos de pacientes que cometeram auto-enucleação (cinco unilaterais e uma bilateral, acompanhados no serviço de psiquiatria do Hospital das Clínicas da Unicamp nos últimos dez anos. Além disso, é feita uma revisão da literatura relacionada a esse tipo de comportamento, considerado a forma mais grave e dramática de automutilação ocular. A partir da discussão crítica comparativa entre os aspectos clínicos de cada caso relatado e os dados encontrados na literatura, concluímos que se mostram associados à auto-enucleação: pacientes esquizofrênicos que cometeram a autolesão durante episódios psicóticos agudos, fortes elementos religiosos como parte da psicopatologia, evidências de comportamento automutilatório menos grave antes da auto-enucleação e explicação do ato como relacionado a uma suposta salvação do próprio paciente ou do mundo. Algumas outras características mais específicas de nossos casos também são destacadas: longo tempo de evolução da doença no momento da auto-enucleação; crenças religiosas variando entre três diferentes igrejas cristãs (Católica, Evangélica e Testemunhas de Jeová; presença de uma forma não usual de auto-enucleação com uso de arma de fogo e presença de um caso de auto-enucleação bilateral.Six cases of self-inflicted ocular mutilation (five unilateral and one bilateral were seen and treated at a university hospital in the last ten years. The literature on ocular self-mutilations was also reviewed. Self-enucleation is considered one of the most severe and dramatic forms of self-mutilation. After comparing these patients' clinical and psychopathological data with the literature on ocular self-enucleation, it was concluded that: 1. It is more frequent in schizophrenic patients during acute episodes; 2. Symptoms with a strong religious content are very important; 3. Less severe forms of self-mutilation before self-enucleation are

  12. Fe(II)–Al(III) layered double hydroxides prepared by ultrasound-assisted co-precipitation method for the reduction of bromate

    Energy Technology Data Exchange (ETDEWEB)

    Zhong, Yu [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082 (China); Yang, Qi, E-mail: yangqi@hnu.edu.cn [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082 (China); Luo, Kun; Wu, Xiuqiong [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082 (China); Li, Xiaoming, E-mail: xmli@hnu.edu.cn [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082 (China); Liu, Yang [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Urban and Rural Garbage Disposal Technology Research Center, Hunan Province, Changsha 410082 (China); Tang, Wangwang; Zeng, Guangming; Peng, Bo [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082 (China)

    2013-04-15

    Highlights: ► Fe(II)–Al(III) LDHs were synthesized by ultrasound-assisted co-precipitation method. ► The Fe–Al (30 min) exhibited highly reduction reactivity on bromate. ► Pseudo-first-order model described the experimental data well. ► The mechanisms of bromate removal were proposed. -- Abstract: Bromate is recognized as an oxyhalide disinfection byproduct in drinking water. In this paper, Fe(II)–Al(III) layered double hydroxides (Fe–Al LDHs) prepared by the ultrasound-assisted co-precipitation method were used for the reduction of bromate in solution. The Fe–Al LDHs particles were characterized by X-ray diffractometer, scanning electron microscopy and thermogravimetry–differential scanning calorimetry. It was found that ultrasound irradiation assistance promoted the formation of the hydrotalcite-like phase and then improved the removal efficiency of bromate. In addition, the effects of solid-to-solution ratio, contact time, initial bromate concentration, initial pH, coexisting anions on the bromate removal were investigated. The results showed the bromate with an initial concentration of 1.56 μmol/L could be completely removed from solution by Fe–Al LDHs within 120 min. When the initial bromate concentration was 7.81 μmol/L, the Fe–Al LDHs with irradiation time of 30 min exhibited the optimum removal efficiency and the bromate removal capacity (q{sub e}) was 6.80 μmol/g. In addition, the appearance of sulfate and production of bromide were observed simultaneously in this process, which suggested that ion-exchange between sulfate and bromate, and the reduction of bromate to bromide by Fe{sup 2+} were the main mechanisms responsible for the bromate removal by Fe–Al LDHs.

  13. Comparison of the exposure rate of wrapped hydroxyapatite (Bio-Eye) versus unwrapped porous polyethylene (Medpor) orbital implants in enucleated patients.

    Science.gov (United States)

    Tabatabaee, Ziaeddin; Mazloumi, Mehdi; Rajabi, Mohammad Taher; Khalilzadeh, Omid; Kassaee, Abolfazl; Moghimi, Sasan; Eftekhar, Hassan; Goldberg, Robert A

    2011-01-01

    To compare the exposure rate of wrapped hydroxyapatite versus unwrapped porous polyethylene orbital implants in enucleated patients. Medical records of the patients who underwent primary placement of hydroxyapatite (Bio-Eye) or porous polyethylene (Medpor) orbital implants after enucleation between 2002 and 2005 in Farabi Eye Hospital were reviewed, and the occurrence of implant exposure during follow-up visits was recorded. The exclusion criteria were secondary implantation, evisceration, or follow up of less than 1 year unless the exposure had occurred in the year after surgery. In the hydroxyapatite group, the implants were wrapped either in Mersilene mesh (65%) or in donor sclera (35%). Wrapping was not performed for any patient in the porous polyethylene group. A total of 198 cases with hydroxyapatite and 53 cases with porous polyethylene implant were identified. The most common causes of enucleation in both groups were globe trauma and painful blind eye. Rate of exposure was significantly higher [odds ratio (OR) = 7.97, p < 0.001] in patients with porous polyethylene (34.0%) than in those with hydroxyapatite implant (6.1%). This association remained significant after adjustment for potential confounders. Mean time of exposure after surgery was significantly (p < 0.001) longer in patients with porous polyethylene implant. Kaplan-Meier plots depicted a significantly (p < 0.001) higher rate of exposure in patients with porous polyethylene implant during the follow-up time. Unwrapped porous polyethylene implants demonstrated a higher rate of exposure, and longer time interval to exposure, compared with wrapped hydroxyapatite implants.

  14. A simple method for rapid microbial identification from positive monomicrobial blood culture bottles through matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Lin, Jung-Fu; Ge, Mao-Cheng; Liu, Tsui-Ping; Chang, Shih-Cheng; Lu, Jang-Jih

    2017-06-30

    Rapid identification of microbes in the bloodstream is crucial in managing septicemia because of its high disease severity, and direct identification from positive blood culture bottles through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can shorten the turnaround time. Therefore, we developed a simple method for rapid microbiological identification from positive blood cultures by using MALDI-TOF MS. We modified previously developed methods to propose a faster, simpler and more economical method, which includes centrifugation and hemolysis. Specifically, our method comprises two-stage centrifugation with gravitational acceleration (g) at 600g and 3000g, followed by the addition of a lysis buffer and another 3000g centrifugation. In total, 324 monomicrobial bacterial cultures were identified. The success rate of species identification was 81.8%, which is comparable with other complex methods. The identification success rate was the highest for Gram-negative aerobes (85%), followed by Gram-positive aerobes (78.2%) and anaerobes (67%). The proposed method requires less than 10 min, costs less than US$0.2 per usage, and facilitates batch processing. We conclude that this method is feasible for clinical use in microbiology laboratories, and can serve as a reference for treatments or further complementary diagnostic testing. Copyright © 2017. Published by Elsevier B.V.

  15. Determination of carbohydrates in tobacco by pressurized liquid extraction combined with a novel ultrasound-assisted dispersive liquid-liquid microextraction method.

    Science.gov (United States)

    Cai, Kai; Hu, Deyu; Lei, Bo; Zhao, Huina; Pan, Wenjie; Song, Baoan

    2015-07-02

    A novel derivatization-ultrasonic assisted-dispersive liquid-liquid microextraction (UA-DLLME) method for the simultaneous determination of 11 main carbohydrates in tobacco has been developed. The combined method involves pressurized liquid extraction (PLE), derivatization, and UA-DLLME, followed by the analysis of the main carbohydrates with a gas chromatography-flame ionization detector (GC-FID). First, the PLE conditions were optimized using a univariate approach. Then, the derivatization methods were properly compared and optimized. The aldononitrile acetate method combined with the O-methoxyoxime-trimethylsilyl method was used for derivatization. Finally, the critical variables affecting the UA-DLLME extraction efficiency were searched using fractional factorial design (FFD) and further optimized using Doehlert design (DD) of the response surface methodology. The optimum conditions were found to be 44 μL for CHCl3, 2.3 mL for H2O, 11% w/v for NaCl, 5 min for the extraction time and 5 min for the centrifugation time. Under the optimized experimental conditions, the detection limit of the method (LODs) and linear correlation coefficient were found to be in the range of 0.06-0.90 μg mL(-1) and 0.9987-0.9999. The proposed method was successfully employed to analyze three flue-cured tobacco cultivars, among which the main carbohydrate concentrations were found to be very different. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Study of nuclear and acrosomal sperm morphometry in ram using a computer-assisted sperm morphometry analysis fluorescence (CASMA-F) method.

    Science.gov (United States)

    Yániz, J L; Capistrós, S; Vicente-Fiel, S; Soler, C; Nuñez de Murga, J; Santolaria, P

    2014-10-01

    The aim of this study was to develop a new method that allows morphometric assessment of the sperm nucleus and acrosome in the ram using fluorescence microscopy and free software. The study was divided into three experiments. In the first experiment, semen smears from 20 ejaculates were fixed and labeled with a propidium iodide-pisum sativum agglutinin (PI/PSA) combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed using the ImageJ program. The computer-assisted sperm morphometry analysis fluorescence (CASMA-F) method used allowed the differentiation, capture, and morphometric analysis of most sperm nuclei, acrosomes, and whole heads with high precision and the assessment of the acrosomal status. In the second experiment, sperm nuclear morphometry by CASMA-F was compared by staining with the PI/PSA combination and staining with Hoechst 33342 as in previous studies. Similar results were obtained using both methods. In the third experiment, CASMA-F with PI/PSA was compared with a more conventional CASMA method (semen smears stained with Hemacolor (HEM) and processed with the ISAS commercial software, HEM). Spermatozoa displayed a bigger size when processed with CASMA-F than with HEM method in all primary sperm head morphometric parameters, but results using both methods were correlated. It was concluded that the CASMA-F method allows the simultaneous assessment of sperm nucleus, acrosome, and head in the ram. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. New method based on combining ultrasonic assisted miniaturized matrix solid-phase dispersion and homogeneous liquid-liquid extraction for the determination of some organochlorinated pesticides in fish

    Energy Technology Data Exchange (ETDEWEB)

    Rezaei, Farahnaz [Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, Narmak, Tehran 16846 (Iran, Islamic Republic of); Hosseini, Mohammad-Reza Milani, E-mail: drmilani@iust.ac.ir [Department of Analytical Chemistry, Faculty of Chemistry, Iran University of Science and Technology, Narmak, Tehran 16846 (Iran, Islamic Republic of); Electroanalytical Chemistry Research Center, Iran University of Science and Technology, Narmak, Tehran 16846 (Iran, Islamic Republic of)

    2011-09-30

    Highlights: {yields} Ultrasonic assisted miniaturized matrix solid-phase dispersion combined with HLLE was developed as a new method for the extraction of OCPs in fish. {yields} The goal of this combination was to enhance the selectivity of HLLE procedure and to extend its application in biological samples. {yields} This method proposed the advantages of good detection limits, lower consumption of reagents, and does not need any special instrumentation. - Abstract: In this study, ultrasonic assisted miniaturized matrix solid-phase dispersion (US-MMSPD) combined with homogeneous liquid-liquid extraction (HLLE) has been developed as a new method for the extraction of organochlorinated pesticides (OCPs) in fish prior to gas chromatography with electron capture detector (GC-ECD). In the proposed method, OCPs (heptachlor, aldrin, DDE, DDD, lindane and endrin) were first extracted from fish sample into acetonitrile by US-MMSPD procedure, and the extract was then used as consolute solvent in HLLE process. Optimal condition for US-MMSPD step was as follows: volume of acetonitrile, 1.5 mL; temperature of ultrasound, 40 deg. C; time of ultrasound, 10 min. For HLLE step, optimal results were obtained at the following conditions: volume of chloroform, 35 {mu}L; volume of aqueous phase, 1.5 mL; volume of double distilled water, 0.5 mL; time of centrifuge, 10 min. Under the optimum conditions, the enrichment factors for the studied compounds were obtained in the range of 185-240, and the overall recoveries were ranged from 39.1% to 81.5%. The limits of detection were 0.4-1.2 ng g{sup -1} and the relative standard deviations for 20 ng g{sup -1} of the OCPs, varied from 3.2% to 8% (n = 4). Finally, the proposed method has been successfully applied to the analysis of the OCPs in real fish sample, and satisfactory results were obtained.

  18. Ultrasound-assisted heating extraction of pectin from grapefruit peel: optimization and comparison with the conventional method.

    Science.gov (United States)

    Wang, Wenjun; Ma, Xiaobin; Xu, Yuting; Cao, Yongqiang; Jiang, Zhumao; Ding, Tian; Ye, Xingqian; Liu, Donghong

    2015-07-01

    The extraction of pectin from grapefruit peel by ultrasound-assisted heating extraction (UAHE) was investigated using response surface methodology and compared with the conventional heating extraction (CHE). The optimized conditions were power intensity of 12.56 W/cm(2), extraction temperature of 66.71°C, and sonication time of 27.95 min. The experimental optimized yield was 27.34%, which was well matched with the predicted value (27.46%). Compared with CHE, UAHE provided higher yield increased by 16.34% at the temperature lowered by 13.3°C and the time shortened by 37.78%. Image studies showed that pectin extracted by UAHE showed better color and more loosen microstructure compared to that extracted by CHE, although Fourier Transform Infrared Analysis indicated insignificant difference in their chemical structures. Furthermore, UAHE pectin possessed lower viscosity, molecular weight and degree of esterification, but higher degree of branching and purity than CHE pectin, indicating that the former was preliminarily modified during the extraction process. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. iContraception(®): a software tool to assist professionals in choosing contraceptive methods according to WHO medical eligibility criteria.

    Science.gov (United States)

    Lopez, Ramón Guisado; Polo, Isabel Ramirez; Berral, Jose Eduardo Arjona; Fernandez, Julia Guisado; Castelo-Branco, Camil

    2015-04-01

    To design software to assist health care providers with contraceptive counselling. The Model-View-Controller software architecture pattern was used. Decision logic was incorporated to automatically compute the safety category of each contraceptive option. Decisions are made according to the specific characteristics or known medical conditions of each potential contraception user. The software is an app designed for the iOS and Android platforms and is available in four languages. iContraception(®) facilitates presentation of visual data on medical eligibility criteria for contraceptive treatments. The use of this software was evaluated by a sample of 54 health care providers. The general satisfaction with the use of the app was over 8 on a 0-10 visual analogue scale in 96.3% of cases. iContraception provides easy access to medical eligibility criteria of contraceptive options and may help with contraceptive counselling. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  20. Heart motion uncertainty compensation prediction method for robot assisted beating heart surgery - Master-slave Kalman Filters approach.

    Science.gov (United States)

    Liang, Fan; Yu, Yang; Cui, Shigang; Zhao, Li; Wu, Xingli

    2014-05-01

    Robot Assisted Coronary Artery Bypass Graft (CABG) allows the heart keep beating in the surgery by actively eliminating the relative motion between point of interest (POI) on the heart surface and surgical tool. The inherited nonlinear and diverse nature of beating heart motion gives a huge obstacle for the robot to meet the demanding tracking control requirements. In this paper, we novelty propose a Master-slave Kalman Filter based on beating heart motion Nonlinear Adaptive Prediction (NAP) algorithm. In the study, we describe the beating heart motion as the combination of nonlinearity relating mathematics part and uncertainty relating non-mathematics part. Specifically, first, we model the nonlinearity of the heart motion via quadratic modulated sinusoids and estimate it by a Master Kalman Filter. Second, we involve the uncertainty heart motion by adaptively change the covariance of the process noise through the slave Kalman Filter. We conduct comparative experiments to evaluate the proposed approach with four distinguished datasets. The results indicate that the new approach reduces prediction errors by at least 30 μm. Moreover, the new approach performs well in robustness test, in which two kinds of arrhythmia datasets from MIT-BIH arrhythmia database are assessed.

  1. Visible light photocatalytic degradation of dyes by bismuth oxide-reduced graphene oxide composites prepared via microwave-assisted method.

    Science.gov (United States)

    Liu, Xinjuan; Pan, Likun; Lv, Tian; Sun, Zhuo; Sun, Chang Q

    2013-10-15

    Bi2O3-reduced graphene oxide (RGO) composites were successfully synthesized via microwave-assisted reduction of graphite oxide in Bi2O3 precursor solution using a microwave system. Their morphologies, structures, and photocatalytic performance in the degradation of methylene blue (MB) and methyl orange (MO) were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction spectroscopy, UV-vis absorption spectroscopy, and electrochemical impedance spectroscopy, respectively. The results show that the RGO addition can enhance the photocatalytic performance of Bi2O3-RGO composites. Bi2O3-RGO composite with 2 wt.% RGO achieves maximum MO and MB degradation rates of 93% and 96% at 240min under visible light irradiation, respectively, much higher than those for the pure Bi2O3 (78% and 76%). The enhanced photocatalytic performance is ascribed to the increased light adsorption and the reduction in electron-hole pair recombination in Bi2O3 with the introduction of RGO. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Phosphate adsorption ability of biochar/Mg-Al assembled nanocomposites prepared by aluminum-electrode based electro-assisted modification method with MgCl₂ as electrolyte.

    Science.gov (United States)

    Jung, Kyung-Won; Jeong, Tae-Un; Hwang, Min-Jin; Kim, Kipal; Ahn, Kyu-Hong

    2015-12-01

    In this work, the textural properties and phosphate adsorption capability of modified-biochar containing Mg-Al assembled nanocomposites prepared by an effective electro-assisted modification method with MgCl2 as an electrolyte have been determined. Structure and chemical analyses of the modified-biochar showed that nano-sized stonelike or flowerlike Mg-Al assembled composites, MgO, spinel MgAl2O4, AlOOH, and Al2O3, were densely grown and uniformly dispersed on the biochar surface. The adsorption isotherm and kinetics data suggested that the biochar/Mg-Al assembled nanocomposites have an energetically heterogeneous surface and that phosphate adsorption could be controlled by multiple processes. The maximum phosphate adsorption capacity was as high as 887 mg g(-1), as fitted by the Langmuir-Freundlich model, and is the highest value ever reported. It was concluded that this novel electro-assisted modification is a very attractive method and the biochar/Mg-Al assembled nanocomposites provide an excellent adsorbent that can effectively remove phosphate from aqueous solutions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. CdS sensitized TiO{sub 2} film for photocatalytic reduction of Cr(VI) by microwave-assisted chemical bath deposition method

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xinjuan; Pan, Likun, E-mail: lkpan@phy.ecnu.edu.cn; Lv, Tian; Sun, Zhuo

    2014-01-15

    Highlights: • CdS sensitized TiO{sub 2} films are synthesized by microwave-assisted deposition method. • The photocatalytic reduction of Cr(VI) by CdS sensitized TiO{sub 2} film is investigated. • A high Cr(VI) reduction rate of 93% is achieved under white LED light irradiation. -- Abstract: CdS quantum dots (QDs) sensitized TiO{sub 2} films were successfully synthesized using one-step microwave assisted chemical bath deposition method, which allows a facile and rapid deposition and integration between CdS QDs and TiO{sub 2} films. The photocatalytic performance of the films fabricated using CdS precursor solutions with different concentrations in the reduction of Cr(VI) under white light emitting diode (LED) light irradiation was investigated. The results show that the CdS sensitization can enhance the photocatalytic performance of TiO{sub 2} film with a maximum reduction rate of 93% for 240 min under white LED light irradiation as compared with the pure TiO{sub 2} film (31%) due to the increase of light absorption and the reduction of recombination of the injected electrons from CdS to TiO{sub 2}.

  4. Synthesis of Co3O4-Bi2O3 using microwave-assisted method as the peroxymonosulfate activator for elimination of bisphenol A.

    Science.gov (United States)

    Hu, Limin; Zhang, Guangshan; Liu, Meng; Wang, Qiao; Wang, Peng

    2017-12-01

    In this work, Co3O4-Bi2O3 was successfully synthesized using a microwave-assisted method [Co3O4-Bi2O3(MW)] and employed as a peroxymonosulfate (PMS) activator for bisphenol A removal. A reference catalyst was prepared using the same preparation conditions but different heating mode and labeled as Co3O4-Bi2O3(CH). The series of Co3O4-Bi2O3 was characterized using XRD, SEM, and N2 adsorption to detect their crystallinity, morphology, and surface area, among others. Results indicated that both microwave and calcination significantly affected the characteristic and catalytic activity of the catalyst. Moreover, the microwave-irradiated catalyst calcined at 300 °C showed higher catalytic activity and mineralization percentage for BPA degradation than the conventionally heated catalyst calcined at the same temperature. Microwave temperature and microwave time of the proposed microwave-assisted method were also investigated. Compared with other catalysts, the present catalyst showed considerably superior preparation time and degradation efficiency. This study broadens a new horizon for advanced oxidation process using a PMS activator.

  5. A prospective, randomized comparison of a 1940 nm and a 2013 nm thulium: yttrium-aluminum-garnet laser device for Thulium VapoEnucleation of the prostate (ThuVEP: First results

    Directory of Open Access Journals (Sweden)

    Christian Tiburtius

    2015-01-01

    Full Text Available Introduction: We report the early postoperative results of the first prospective, randomized comparison of two commercially available thulium lasers with different wavelengths for the treatment of benign prostatic obstruction (BPO. Materials and Methods: From January to June 2013, 80 consecutive patients were randomized for Thulium VapoEnucleation of the prostate (ThuVEP with a 2013 nm (RevoLix ® (n = 39 or a 1940 nm (Vela ® XL (n = 41 thulium laser. Preoperative status, surgical details and the immediate outcome were recorded for each patient. The perioperative complications were assessed and classified according to the modified Clavien classification system. Results: Median operation time, resected tissue, percentage of resected tissue, catheter time, overall operation efficiency and Hb loss differed nonsignificantly between both devices (P > 0.05. At discharge, the median maximum urinary flow rate and postvoiding residual (PVR urine improved significantly in both groups (P < 0.001. The PVR was lower in the 1940 nm ThuVEP group (P ≤ 0.034. Perioperative complications occurred in 18 (22.5% patients (Clavien 1: 12.5%; Clavien 2: 5%, Clavien 3b: 2.5%, Clavien 4a: 2.5%, with no differences between the groups (P = 0.5. Conclusions: The 1940 nm and the 2013 nm thulium lasers are both safe and effective for the treatment of BPO with ThuVEP. Both lasers give equivalent and satisfactory immediate micturition improvement with low perioperative morbidity.

  6. Housing Assistance

    Directory of Open Access Journals (Sweden)

    Emma Baker

    2013-07-01

    Full Text Available In Australia, an increasing number of households face problems of access to suitable housing in the private market. In response, the Federal and State Governments share responsibility for providing housing assistance to these, mainly low-income, households. A broad range of policy instruments are used to provide and maintain housing assistance across all housing tenures, for example, assisting entry into homeownership, providing affordability assistance in the private rental market, and the provision of socially owned and managed housing options. Underlying each of these interventions is the premise that secure, affordable, and appropriate housing provides not only shelter but also a number of nonshelter benefits to individuals and their households. Although the nonshelter outcomes of housing are well acknowledged in Australia, the understanding of the nonshelter outcomes of housing assistance is less clear. This paper explores nonshelter outcomes of three of the major forms of housing assistance provided by Australian governments—low-income mortgage assistance, social housing, and private rent assistance. It is based upon analysis of a survey of 1,353 low-income recipients of housing assistance, and specifically measures the formulation of health and well-being, financial stress, and housing satisfaction outcomes across these three assistance types. We find clear evidence that health, finance, and housing satisfaction outcomes are associated with quite different factors for individuals in these three major housing assistance types.

  7. BCT phase formation in synthesis via microwave assisted hydrothermal method; Limite da concentracao de Ca na formacao da fase BCT em sintese via metodo hidrotermico assistido por microondas

    Energy Technology Data Exchange (ETDEWEB)

    Barra, B.C.; Souza, A.E.; Teixeira, S.R.; Santos, G.T.A.; Lanzi, C.A.C. [Universidade Estadual Paulista Julio de Mesquita Filho (FCT/DFQB/UNESP), Presidente Prudente, SP (Brazil). Fac. de Ciencia e Tecnologia. Dept. de Fisica, Quimica e Biologia; Longo, E. [Universidade Estadual Paulista Julio de Mesquita Filho (IQ/UNESP/), Araraquara, SP (Brazil); Instituto Nacional de Ciencias e Tecnologia de Materiais em Nanotecnologia (INCTMN), Araraquara, SP (Brazil)

    2012-07-01

    In previous work, samples of barium and calcium titanate (Ba1-xCaxTiO3 (BCT x = 0- 1) were prepared using the microwave assisted hydrothermal method in conditions of relatively short time and temperature. To the sample with 75wt% of Ca no BCT phase was formed but the photoluminescent emission was improved. In the present study, these titanates were synthesized by the same method with other concentrations of Ca, Ba1-xCaxTiO3 (x = 0, 0.20, 0.40, 0. 60, 0.80 and 1) to evaluate the limit of BCT phase formation. Results of X-ray diffraction showed that the phase BCT is formed between zero and 50wt%-Ca, in Ba substitution. Above this concentration, was observed only the formation of carbonates, and to x = 1 there was carbonate formation together with CaTiO3. These results were confirmed by micro Raman spectroscopy. (author)

  8. Application of Taguchi Method to the Optimization of a-C:H Coatings Deposited Using Ion Beam Assisted Physical Vapor Deposition

    Directory of Open Access Journals (Sweden)

    W. H. Kao

    2015-02-01

    Full Text Available The Taguchi design method is used to optimize the adhesion, hardness, and wear resistance properties of a-C:H coatings deposited on AISI M2 steel substrates using the ion beam assisted physical vapor deposition method. The adhesion strength of the coatings is evaluated by means of scratch tests, while the hardness is measured using a nanoindentation tester. Finally, the wear resistance is evaluated by performing cyclic ball-on-disc wear tests. The Taguchi experimental results show that the optimal deposition parameters are as follows: a substrate bias voltage of 90 V, an ion beam voltage of 1 kV, an acetylene flow rate of 21 sccm, and a working distance of 7 cm. Given these optimal processing conditions, the a-C:H coating has a critical load of 99.8 N, a hardness of 25.5 GPa, and a wear rate of 0.4 × 10−6 mm3/Nm.

  9. Sample-first preparation: a method for surface-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cyclic oligosaccharides.

    Science.gov (United States)

    Wu, Hsin-Pin; Su, Chih-Lin; Chang, Hui-Chiu; Tseng, Wei-Lung

    2007-08-15

    A new sample preparation method for the analysis of cyclic oligosaccharides in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is presented. We call this new technique "sample first method", in which a sample is deposited first and then bare gold nanoparticles (AuNPs), which serve as the SALDI matrixes, are added to the top of the sample layer. The use of the sample first method offers significant advantages for improving shot-to-shot reproducibility, enhancing the ionization efficiency of the analyte, and reducing sample preparation time as compared to the dried-droplet method, wherein samples and bare AuNPs are mixed and dried together. The relative standard deviation (RSD) values of the signal intensity as calculated from 65 sample spots was 25% when the sample first methods were applied to the analysis of beta-cyclodextrin. The results were more homogeneous as compared to the outcome using dried-droplet preparation of AuNPs (RSD=66%) and 2,5-dihydroxybenzoic acid (RSD=209%). We also found out that the optimal concentration of AuNP for ionization efficiency is 7.4 nM (4.52x10(12) particles/mL) while the lowest detectable concentration of cyclic oligosaccharides through this approach is 0.25 microM. Except for the cyclic oligosaccharide, the proposed method was also applied to the analyses of other biological samples, including neutral carbohydrate and steroid, aminothiols, and peptides as well as proteins.

  10. Optimized design method for trench-assisted grade-index ring-core fiber with low DMD and large Aeff

    Science.gov (United States)

    Qiao, Xihui; Tu, Jiajing; Long, Keping

    2017-02-01

    We propose a kind of trench-assisted graded-index ring-core fiber (TA-GI-RCF) with a low refractive index rod deployed in the center of the core, which supports three LP modes (LP01, LP11 and LP21) transmission. There are two difficulties about designing TA-GI-RCF, one is to depart LP21 mode from LP02 mode because their effective indices are too close which makes it difficult to realize only three LP modes transmission; the other one is how to make sure these three LP modes reach the receiver end with low differential mode delay (DMD), so that the computation complexity of multi-input multi-output (MIMO) digital signal process (DSP) can be reduced. At first, we realize the separation of LP21 mode and LP02 mode in TA-GI-RCF by enlarging the size of low refractive index rod. We next investigate the influence of the TA-GI-RCF structural parameters on DMD and DMD slope, and find that a graded-index core and a low refractive index rod can flexibly tune the DMD, and a trench can flexibly control the DMD slope. Through optimizing the core parameters, we find that the design region of α is 1.01 2.23 and that of Δ1 is 0.28% 0.46% at r1=30 μm, where α is the profile exponent and Δ1 is the relative refractive index difference between core and cladding. Simulation results show that TA-GI-RCF can achieve the effective area (Aeff) of LP01 mode over 2000 μm2 and the |DMD| between LP01 mode and LP11 mode is DMD over whole C+L band and large Aeff.

  11. Conserving analyst attention units: use of multi-agent software and CEP methods to assist information analysis

    Science.gov (United States)

    Rimland, Jeffrey; McNeese, Michael; Hall, David

    2013-05-01

    Although the capability of computer-based artificial intelligence techniques for decision-making and situational awareness has seen notable improvement over the last several decades, the current state-of-the-art still falls short of creating computer systems capable of autonomously making complex decisions and judgments in many domains where data is nuanced and accountability is high. However, there is a great deal of potential for hybrid systems in which software applications augment human capabilities by focusing the analyst's attention to relevant information elements based on both a priori knowledge of the analyst's goals and the processing/correlation of a series of data streams too numerous and heterogeneous for the analyst to digest without assistance. Researchers at Penn State University are exploring ways in which an information framework influenced by Klein's (Recognition Primed Decision) RPD model, Endsley's model of situational awareness, and the Joint Directors of Laboratories (JDL) data fusion process model can be implemented through a novel combination of Complex Event Processing (CEP) and Multi-Agent Software (MAS). Though originally designed for stock market and financial applications, the high performance data-driven nature of CEP techniques provide a natural compliment to the proven capabilities of MAS systems for modeling naturalistic decision-making, performing process adjudication, and optimizing networked processing and cognition via the use of "mobile agents." This paper addresses the challenges and opportunities of such a framework for augmenting human observational capability as well as enabling the ability to perform collaborative context-aware reasoning in both human teams and hybrid human / software agent teams.

  12. Structural and physicochemical properties of nickel manganite NiMn{sub 2}O{sub 4-δ}synthesized by sol-gel and ultra sound assisted methods

    Energy Technology Data Exchange (ETDEWEB)

    Alburquenque, D. [Materials Chemistry Department, USACH, Ave L.B.O' Higgins 3363, Santiago 9170022 (Chile); Metallurgy Department, USACH, Ave Ecuador 3469, Santiago 9170124 (Chile); Troncoso, L.; Denardin, J.C. [Physics Department, USACH, Ave. Ecuador 3493, Santiago 9170124 (Chile); Butera, A. [Atomic Center, CNEA, Ito. Balseiro, Bariloche Rio Negro 84000 (Argentina); Padmasree, K.D. [Cinvestav, U.Saltillo, Monterrey Km 13.5, Saltillo CP 25900 (Mexico); Ortiz, J.; Herrera, F. [Materials Chemistry Department, USACH, Ave L.B.O' Higgins 3363, Santiago 9170022 (Chile); Marco, J.F. [Institute of Physical Chemistry “Rocasolano”, CSIC, Serrano 119, Madrid 28006 (Spain); Gautier, J.L., E-mail: juan.gautier@usach.cl [Materials Chemistry Department, USACH, Ave L.B.O' Higgins 3363, Santiago 9170022 (Chile)

    2016-07-05

    In this work we present the structural, magnetic and surface characterization of the ceramic nickel manganite oxide NiMn{sub 2}O{sub 4-δ} synthesized by two methods: sol-gel and ultrasound-assisted sol-gel using nitrate salts as precursors. We have characterized the non-stoichiometric samples NiMn{sub 2}O{sub 4-δ} using different physicochemical analyses. X-ray diffraction and Rietveld refinement of the X-ray data indicated that the samples crystallize in the Fd3m space group characteristic of cubic spinel-related oxides. The specific surface area of the oxides was 1.3 m{sup 2}/g (SG) and 16.3 m{sup 2}/g (UASG). SEM results showed particle agglomerates of 1.05 μm (SG) and 0.85 μm (UASG). Temperature dependence magnetization measurements were performed and a ferrimagnetic transition was identified at 103 K and 105 K depending on the preparation method (SG and UASG, respectively). The observed Curie constant was found to vary from 7.4 to 7.7 cm{sup 3} K mol{sup −1} and hysteretic magnetization vs. applied field curves at different temperatures were obtained. XPS studies of these oxides reveal the presence of Ni{sup 2+}, Mn{sup 2+}, Mn{sup 3+} and Mn{sup 4+} ions at the surface. A probable ionic distribution as a function of the synthesis method is proposed. - Highlights: • Nickel manganite was synthesized by sol gel synthesis and a novel ultrasound-assisted sol gel method. . • Bulk and surface cation distributions are proposed. • Physicochemical properties, including magnetic and surface area measurements, were studied for both types of samples.

  13. Low-temperature synthesis and characterization of anatase TiO{sub 2} nanoparticles by an acid assisted sol–gel method

    Energy Technology Data Exchange (ETDEWEB)

    Leyva-Porras, C. [Centro de Investigación en Materiales DIP-CUCEI, Universidad de Guadalajara, Av. Revolución # 1500, Col. Olímpica, C.P. 44430, Guadalajara (Mexico); Centro de Investigación en Materiales Avanzados, S.C. (CIMAV), Alianza Norte No. 202, Parque de Investigación e Innovación Tecnológica (PIIT), Carretera Aeropuerto km. 10, C.P. 66600, Apodaca, N.L. (Mexico); Toxqui-Teran, A.; Vega-Becerra, O. [Centro de Investigación en Materiales Avanzados, S.C. (CIMAV), Alianza Norte No. 202, Parque de Investigación e Innovación Tecnológica (PIIT), Carretera Aeropuerto km. 10, C.P. 66600, Apodaca, N.L. (Mexico); Miki-Yoshida, M. [Centro de Investigación en Materiales Avanzados, S.C. (CIMAV), Miguel de Cervantes No. 120, Parque Industrial Chihuahua, C.P. 31109, Chihuahua (Mexico); Rojas-Villalobos, M.; García-Guaderrama, M. [Centro de Investigación en Materiales DIP-CUCEI, Universidad de Guadalajara, Av. Revolución # 1500, Col. Olímpica, C.P. 44430, Guadalajara (Mexico); and others

    2015-10-25

    The synthesis of anatase TiO{sub 2} nanoparticles by an acid-assisted sol–gel method at 25 and 80 °C is described. Specifically, acetic acid (AA) was used and the evolution of the anatase phase with the amount of AA was observed. The results of X-ray diffraction (XRD) and transmission electron microscopy (TEM) both showed that a pure anatase phase was obtained with particle size smaller than 5 nm. Structural refinements and quantitative determination of phase composition was achieved by using the Rietveld method. The particle size distribution became slightly narrower as the amount of AA was increased. Raman spectroscopy showed that when the amount of AA was increased a small amount of brookite was present at the contamination level. The anatase phase was studied by differential thermal analysis (DTA), providing phase stability up to 600 °C. These and other results were discussed in terms of particle size and structure. Likewise, the formation of the anatase phase under these synthesis conditions was explained. - Highlights: • Synthesis of anatase TiO{sub 2} nanoparticles by an acid assisted sol–gel method at mild conditions. • Microstructure characterization by XRD, TEM and Raman spectroscopy. • Observation of the formation and evolution of the anatase phase as acetic acid was increased. • Anatase thermal stability up to 600 °C and band gap range between 3.2 and 3.5 eV. • A simplified method which can be considered as a green chemistry process.

  14. HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule

    Science.gov (United States)

    Hadad, Ghada M.; El-Gindy, Alaa; Mahmoud, Waleed M. M.

    2008-08-01

    High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase C 18 analytical column with a mobile phase consisting of