WorldWideScience

Sample records for assaying qualitative

  1. Application of statistical process control to qualitative molecular diagnostic assays

    LENUS (Irish Health Repository)

    O'Brien, Cathal P.

    2014-11-01

    Modern pathology laboratories and in particular high throughput laboratories such as clinical chemistry have developed a reliable system for statistical process control (SPC). Such a system is absent from the majority of molecular laboratories and where present is confined to quantitative assays. As the inability to apply SPC to an assay is an obvious disadvantage this study aimed to solve this problem by using a frequency estimate coupled with a confidence interval calculation to detect deviations from an expected mutation frequency. The results of this study demonstrate the strengths and weaknesses of this approach and highlight minimum sample number requirements. Notably, assays with low mutation frequencies and detection of small deviations from an expected value require greater sample numbers to mitigate a protracted time to detection. Modeled laboratory data was also used to highlight how this approach might be applied in a routine molecular laboratory. This article is the first to describe the application of SPC to qualitative laboratory data.

  2. How Do Detergents Work? A Qualitative Assay to Measure Amylase Activity

    Science.gov (United States)

    Novo, M. Teresa; Casanoves, Marina; Garcia-Vallvé, Santi; Pujadas, Gerard; Mulero, Miquel; Valls, Cristina

    2016-01-01

    We present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a…

  3. A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue

    Directory of Open Access Journals (Sweden)

    Bodil Hakonen

    2014-01-01

    Full Text Available The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH agar or 3D synthetic soft tissues (SST and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.

  4. Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

    Science.gov (United States)

    Libert, X; Chasseur, C; Bladt, S; Packeu, A; Bureau, F; Roosens, N H; De Keersmaecker, S C J

    2015-09-01

    Currently, contamination of indoor environment by fungi and molds is considered as a public health problem. The monitoring of indoor airborne fungal contamination is a common tool to help understanding the link between fungi in houses and respiratory problems. Classical analytical monitoring methods, based on cultivation and microscopic identification, depend on the growth of the fungi. Consequently, they are biased by difficulties to grow some species on certain culture media and under certain conditions or by noncultivable or dead fungi that can consequently not be identified. However, they could have an impact on human health as they might be allergenic. Since molecular methods do not require a culture step, they seem an excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR® green real-time PCR-based assay for the specific detection and identification of Aspergillus versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and repeatability. The limit of detection was determined to be 1 or 2 copies of genomic DNA of A. versicolor. In order to demonstrate that this SYBR® green qPCR assay is a valuable alternative for monitoring indoor fungal contamination with A. versicolor, environmental samples collected in contaminated houses were analyzed and the results were compared to the ones obtained with the traditional methods. PMID:26184975

  5. Fluid-phase immunoradiometric assay for the detection of qualitative abnormalities of factor VIII/von Willebrand factor in variants of von Willebrand's disease

    International Nuclear Information System (INIS)

    Antigenic reactivity of F.VIII/WF in variants of von Willebrand's disease (vWd) was studied with both fluid-phase and solid-phase immunoradiometric assays. Two different (rabbit and goat) 125I-labeled specific antibodies against purified F.VIII/WF were used in both their divalent (lgG) and their monovalent (Fab fragment) forms. Dose-response curves obtained by reacting a constant amount of antibody with serial dilutions of plasmas from normal or homozygous vWd demonstrated the specificity of the test. The accuracy was significantly higher with 125I-Fab fragments of goat anti-F.VIII/WF antiserum than intact goat lgG or rabbit lgG or Fab fragments. The significant decrease of the slope of the dose-response curves obtained with plasma from variants of vWd has been interpreted as due to the presence of abnormal F.VIII/WF molecules with decreased antigenic reactivity. A similar anomaly was found in cryosupernatant prepared from normal plasma, paralleling similarities demonstrated between variants of vWd and cryosupernatant. Results of experiments performed by reacting constant plasma dilutions from control or variants of vWd and varying concentrations of anti-F.VIII/WF Fab fragments (rabbit or goat) confirmed the decreased antigenic reactivity of variant F.VIII/WF

  6. New Application of the Comet Assay: Chromosome–Comet Assay

    OpenAIRE

    Cortés-Gutiérrez, Elva I.; DÁVILA-RODRÍGUEZ, MARTHA I.; Fernández, José Luís; López-Fernández, Carmen; Gosálbez, Altea; Gosálvez, Jaime

    2011-01-01

    The comet assay is a well-established, simple, versatile, visual, rapid, and sensitive tool used extensively to assess DNA damage and DNA repair quantitatively and qualitatively in single cells. The comet assay is most frequently used to analyze white blood cells or lymphocytes in human biomonitoring studies, although other cell types have been examined, including buccal, nasal, epithelial, and placental cells and even spermatozoa. This study was conducted to design a protocol that can be use...

  7. Qualitative Economics

    DEFF Research Database (Denmark)

    Fast, Michael; Clark II, Woodrow W

    2013-01-01

    This chapter is about science from a book that on Qualitative Economics (Clark and Fast 2008), specifically building a science of economics, grounded in understanding of organizations and what is beneath the surface of structures and activities. Economics should be, as a science, concerned with its...... assumptions and how to develop and formulate theories of ideas and reality that produce descriptions of how to understand phenomenon that create experiences, hypotheses generation and replicable data which need to be connected to everyday business life. Economics has to start with a discussion of philosophy...... of science There is a “disconnection” between economics which focuses on structures and universal laws from those that are in contrast with the everyday of life of business activity, which are processual and dynamic. This discussion is the central issue in the paper, and is discussed from the perspective...

  8. Qualitative Economics

    DEFF Research Database (Denmark)

    Fast, Michael; Clark II, Woodrow W

                         This book is about science -- specifically, the science of economics. Or lack thereof is more accurate. The building of any science, let alone economics, is grounded in the understanding of what is beneath the "surface" of economics. Science, and hence economics, should...... be concerned with formulating ideas that express theories which produce descriptions of how to understand phenomenon and real world experiences.                       Economics must become a science, because the essence of economics in terms of human actions, group interactions and communities are in need...... of scientific inquiry. Academics and scholars need a scientific perspective that can hypothesize, theorize document, understand and analyze human dynamics from the individual to more societal interactions. And that is what qualitative economics does; it can make economics into becoming a science. The economic...

  9. Hormone assay

    International Nuclear Information System (INIS)

    An improved radioimmunoassay is described for measuring total triiodothyronine or total thyroxine levels in a sample of serum containing free endogenous thyroid hormone and endogenous thyroid hormone bound to thyroid hormone binding protein. The thyroid hormone is released from the protein by adding hydrochloric acid to the serum. The pH of the separated thyroid hormone and thyroid hormone binding protein is raised in the absence of a blocking agent without interference from the endogenous protein. 125I-labelled thyroid hormone and thyroid hormone antibodies are added to the mixture, allowing the labelled and unlabelled thyroid hormone and the thyroid hormone antibody to bind competitively. This results in free thyroid hormone being separated from antibody bound thyroid hormone and thus the unknown quantity of thyroid hormone may be determined. A thyroid hormone test assay kit is described for this radioimmunoassay. It provides a 'single tube' assay which does not require blocking agents for endogenous protein interference nor an external solid phase sorption step for the separation of bound and free hormone after the competitive binding step; it also requires a minimum number of manipulative steps. Examples of the assay are given to illustrate the reproducibility, linearity and specificity of the assay. (UK)

  10. Radioreceptor assays

    International Nuclear Information System (INIS)

    Radioreceptor assay (RRA) is an analytical method using the specific interaction of some pharmaceuticals and endogenic substances (ligands) with specific receptors present in certin tissues of living organisms. RRA uses the principle of isotope dilution. The method is described in detail of the preparation of receptors, samples and radioligands, conditions of incubation, the separation of free and bound radioligand, and the mathematical evaluation of RRA. The sensitivity of RRA is measured in units to tens of pg. The specificity of RRA relates to a group of substances with similar pharmacological effect. RRA may be used for identifying neuroleptics, antidepressants, anxiolytics, ergot alkaloids, beta blockers, anticholinergic drugs, certain hormones and neuropeptides. (M.D.)

  11. Angiogenesis Assays.

    Science.gov (United States)

    Nambiar, Dhanya K; Kujur, Praveen K; Singh, Rana P

    2016-01-01

    Neoangiogenesis constitutes one of the first steps of tumor progression beyond a critical size of tumor growth, which supplies a dormant mass of cancerous cells with the required nutrient supply and gaseous exchange through blood vessels essentially needed for their sustained and aggressive growth. In order to understand any biological process, it becomes imperative that we use models, which could mimic the actual biological system as closely as possible. Hence, finding the most appropriate model is always a vital part of any experimental design. Angiogenesis research has also been much affected due to lack of simple, reliable, and relevant models which could be easily quantitated. The angiogenesis models have been used extensively for studying the action of various molecules for agonist or antagonistic behaviour and associated mechanisms. Here, we have described two protocols or models which have been popularly utilized for studying angiogenic parameters. Rat aortic ring assay tends to bridge the gap between in vitro and in vivo models. The chorioallantoic membrane (CAM) assay is one of the most utilized in vivo model system for angiogenesis-related studies. The CAM is highly vascularized tissue of the avian embryo and serves as a good model to study the effects of various test compounds on neoangiogenesis. PMID:26608294

  12. Conflicts in qualitative research

    OpenAIRE

    Colmenero Ruiz, María Jesús; Domínguez Garrido, María Concepción; Huber, Günter L.; Jiménez-Garrido, Amador; Khan, Shahjehan; Ernesto LÓPEZ GÓMEZ; Medina Rivilla, Antonio; Medina Domínguez, María; Medina Domínguez, Conchita; Pegalajar Palomino, María del Carmen; Pérez Navío, Eufrasio; Santoro Moreno, José María; Sládková, Jana; Center for Qualitative Psychology e.V.

    2014-01-01

    Volume 12 of the Qualitative Psychology Nexus series presents contributions to the XIV. Workshop on Qualitative Research in Psychology on the general topic of "Conflicts in Qualitative Research." It was organized by the Center for Qualitative Psychology and the Department of Pedagogy of the University of Jaén, Spain, during May 17-19, 2013. The presentations cover a broad spectrum of possible conflicts that social researchers using qualitative methods have to overcome, ranging from elabora...

  13. Qualitative Research Process

    OpenAIRE

    Dewan Mahboob HOSSAIN

    2011-01-01

    This article provides with an overview of the qualitative research methods. Over last few decades, qualitative research is getting very popular in the fields of business, sociology, psychology and others. This article, in its introduction, gives a general idea about the qualitative research. Then it discusses the main differences between qualitative and quantitative research methods. The article also discusses about the ethical issues important for qualitative research. Lastly it discusses ab...

  14. Qualitative research in health

    OpenAIRE

    Ligia Regina Franco Sansigolo Kerr; Carl Kendall

    2014-01-01

    Qualitative research and health has become extremely popular in the last 30 years. Since the 80’s, more and more health professionals have engaged in qualitative research. Discriminating a “qualitative research” from quantitative research, though, is a misnomer, since all research is at least part qualitative. After all, when epidemiologists or biostatisticians count something, that category is a qualitative “something”.

  15. Radiopharmaceutical assays

    International Nuclear Information System (INIS)

    Under the laws in force, radiopharmaceuticals for human use must be among other features, non-pyrogenous and non-toxic. For this reason pyrogenity and toxicity assays are carried out. Pharmacokinetic studies may also be necessary in some cases. Products currently made at the Radiopharmaceutical Center, new products designed for certification and raw materials used to manufacture the above, were tested. A total 342 pyrogenity and toxicity tests, and four pharmacokinetic studies were conducted in 1996. To determine pyrogenity, the temperature animals were measured following intravenous administration of radiopharmaceuticals concerned: sodium pertechnetate, colloidal gold and sodium orthoradiohippurate from current production; pharmaceutic components of several new products, i.e. technetium generator, fibrinogen and microspheres. A total 327 products were tested, 96 percent of which met the requirements. To determine toxicity, the probit method was used, consisting of the administration of radiopharmaceutical doses for seven straight days, and checking for lethal effects. An overall 15 tests were carried out and 80 percent of products tested were found certifiable. Pharmacokinetic tests consisted of tropism on target organs and biodistribution in several organs using the tomographic method. (author)

  16. Validity in qualitative research

    OpenAIRE

    Kuzmanić, Marja

    2015-01-01

    This paper addresses several issues related to validity in qualitative research and, more specifically, explores the ways in which validity has been discussed and applied in research with qualitative interviews. The central question is to what extent, if at all, traditional positivist validity criteria are applicable, but also relevant, for evaluation of research with qualitative interviewing. The qualitative interview has been chosen as the focal point of this paper because of its peculiarit...

  17. Qualitative Research in Psychology

    OpenAIRE

    Fattah Hanurawan

    2013-01-01

    "The first 'Workshop Qualitative Research in Psychology' took place in Blaubeuren, Germany from October 20-22, 2000. The meeting was organized by the Center for Qualitative Psychology of the University of Tübingen, Germany. The purpose of the meeting was to begin a network of qualitative psychologists. Thirty-two participants got to know each other, presented and discussed their research, discussed potential further developments within the field of qualitative psychology, and inspired each ot...

  18. The qualitative research proposal

    OpenAIRE

    H. Klopper

    2008-01-01

    Qualitative research in the health sciences has had to overcome many prejudices and a number of misunderstandings, but today qualitative research is as acceptable as quantitative research designs and is widely funded and published. Writing the proposal of a qualitative study, however, can be a challenging feat, due to the emergent nature of the qualitative research design and the description of the methodology as a process. Even today, many sub-standard proposals at post-graduate evaluation c...

  19. Effectively Communicating Qualitative Research

    Science.gov (United States)

    Ponterotto, Joseph G.; Grieger, Ingrid

    2007-01-01

    This article is a guide for counseling researchers wishing to communicate the methods and results of their qualitative research to varied audiences. The authors posit that the first step in effectively communicating qualitative research is the development of strong qualitative research skills. To this end, the authors review a process model for…

  20. Qualitative Research Methods

    OpenAIRE

    Yıldırım, Ali; Orta Doğu Teknik Üniversitesi Eğitim Bilimleri Bölümü

    1999-01-01

    In this article, the basic characteristics of qualitative research methods are discussed in relation to quantitative research methods and their potential contribution to educational research. In addition, the article reviews the roots of qualitative research, the basic data collection methods used and how the results are used in practice. As the basic characteristics of qualitative research, being sensitive to natural context, the participant role of the researcher, a holistic approach, explo...

  1. Truly quantitative analysis of the firefly luciferase complementation assay

    Directory of Open Access Journals (Sweden)

    Renee Dale

    2016-04-01

    Full Text Available Luciferase complementation assays detect protein-protein interactions within living cells using bioluminescence. Since the first report using plant cells was published in 2007, over 100 peer-reviewed articles have been published describing the detection of protein-protein interactions within plant cells by the assays. The assays have also been used to analyze networks of protein-protein interactions in plants. Although the assays have a high dynamic range, they remain qualitative with respect to determining the affinities of interactions. In this article, we first summarize the luciferase complementation assays developed in the past years. We then describe the mechanism of the firefly luciferase complementation that is most widely used in plants, and the reason it is qualitative rather than quantitative using a mathematical model. Finally, we discuss possible procedures to quantitatively determine the affinity of a protein pair using the firefly luciferase complementation assay.

  2. Computers and Qualitative Research.

    Science.gov (United States)

    Willis, Jerry; Jost, Muktha

    1999-01-01

    Discusses the use of computers in qualitative research, including sources of information; collaboration; electronic discussion groups; Web sites; Internet search engines; electronic sources of data; data collection; communicating research results; desktop publishing; hypermedia and multimedia documents; electronic publishing; holistic and…

  3. Qualitative research for international business

    OpenAIRE

    Yves Doz

    2011-01-01

    Qualitative research in international business has been rare, the main research streams of the field relying more on quantitative methods. This paper first outlines why qualitative research has been scant. It then presents areas, such as theory building, where qualitative research could make a substantial contribution. Third, it reviews approaches to high standards of qualitative research and criteria for evaluating qualitative research. Finally, some possible research areas where qualitative...

  4. Qualitative Robustness in Estimation

    Directory of Open Access Journals (Sweden)

    Mohammed Nasser

    2012-07-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Times New Roman","serif";} Qualitative robustness, influence function, and breakdown point are three main concepts to judge an estimator from the viewpoint of robust estimation. It is important as well as interesting to study relation among them. This article attempts to present the concept of qualitative robustness as forwarded by first proponents and its later development. It illustrates intricacies of qualitative robustness and its relation with consistency, and also tries to remove commonly believed misunderstandings about relation between influence function and qualitative robustness citing some examples from literature and providing a new counter-example. At the end it places a useful finite and a simulated version of   qualitative robustness index (QRI. In order to assess the performance of the proposed measures, we have compared fifteen estimators of correlation coefficient using simulated as well as real data sets.

  5. Qualitative Assertions as Prescriptive Statements

    Science.gov (United States)

    Nolen, Amanda; Talbert, Tony

    2011-01-01

    The primary question regarding prescriptive appropriateness is a difficult one to answer for the qualitative researcher. While there are certainly qualitative researchers who have offered prescriptive protocols to better define and describe the terrain of qualitative research design and there are qualitative researchers who offer research…

  6. 21 CFR 866.3980 - Respiratory viral panel multiplex nucleic acid assay.

    Science.gov (United States)

    2010-04-01

    ... assay. 866.3980 Section 866.3980 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... § 866.3980 Respiratory viral panel multiplex nucleic acid assay. (a) Identification. A respiratory viral panel multiplex nucleic acid assay is a qualitative in vitro diagnostic device intended...

  7. Demystifying Interdisciplinary Qualitative Research

    Science.gov (United States)

    Greckhamer, Thomas; Koro-Ljungberg, Mirka; Cilesiz, Sebnem; Hayes, Sharon

    2008-01-01

    This article seeks to demystify, through deconstruction, the concept of "interdisciplinarity" in the context of qualitative research to contribute to a new praxis of knowledge production through reflection on the possibilities and impossibilities of interdisciplinarity. A review and discussion of disciplinarity and interdisciplinarity leads the…

  8. Disciplining Qualitative Research

    Science.gov (United States)

    Denzin, Norman K.; Lincoln, Yvonna S.; Giardina, Michael D.

    2006-01-01

    Qualitative research exists in a time of global uncertainty. Around the world, governments are attempting to regulate scientific inquiry by defining what counts as "good" science. These regulatory activities raise fundamental, philosophical epistemological, political and pedagogical issues for scholarship and freedom of speech in the academy. This…

  9. The Qualitative Similarity Hypothesis

    Science.gov (United States)

    Paul, Peter V.; Lee, Chongmin

    2010-01-01

    Evidence is presented for the qualitative similarity hypothesis (QSH) with respect to children and adolescents who are d/Deaf or hard of hearing. The primary focus is on the development of English language and literacy skills, and some information is provided on the acquisition of English as a second language. The QSH is briefly discussed within…

  10. Microbead agglutination based assays

    KAUST Repository

    Kodzius, Rimantas

    2013-01-21

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

  11. Radioreceptor opioid assay

    International Nuclear Information System (INIS)

    A radioreceptor assay is described for assaying opioid drugs in biological fluids. The method enables the assay of total opioid activity, being specific for opioids as a class but lacking specificity within the class. A radio-iodinated opioid and the liquid test sample are incubated with an opiate receptor material. The percentage inhibition of the binding of the radio-iodinated compound to the opiate receptor is calculated and the opioid activity of the test liquid determined from a standard curve. Examples of preparing radio-iodinated opioids and assaying opioid activity are given. A test kit for the assay is described. Compared to other methods, this assay is cheap, easy and rapid. (U.K.)

  12. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K.; Snyderman, Neal J.; Rowland, Mark S.

    2010-07-13

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  13. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K.; Snyderman, Neal J.; Rowland, Mark S.

    2012-05-15

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  14. QSMSR QUALITATIVE MODEL

    Directory of Open Access Journals (Sweden)

    Tahir Abdullah

    2012-02-01

    Full Text Available Software architecture design and requirement engineering are core and independent areas of engineering. A lot of research, education and practice are carried on Requirement elicitation and doing refine it, but it is a major issue of engineering. QSMSR model act as a bridge between requirement and design there is a huge gap between these two areas of software architecture and requirement engineering. The QSMSR model divide into two sub model qualitative model and Principal model in this research we focus on Qualitative model which further divide into two sub models fabricated model and classified model. Classified model make the sub groups of the role and match it with components. The Fabricated model link QSMSR Principal Model to an architecture design. At the end it provides the QSMSR Architecture model of the system as output.

  15. Tricyclic antidepressant radioreceptor assay

    International Nuclear Information System (INIS)

    A receptor assay for tricyclic antidepressants described here is based on the ability of these drugs to compete with [3H]-3-guinuclidnyl benzilate (3H-QNB) for binding to muscarinic cholinergic receptors in rat brain membranes. The assay is sensitive, in that it can detect, for example, 2ng/ml nortriptyline in plasma. Seven plasma samples from depressed patients treated with nortriptyline were assayed with the radioreceptor and gas liquid chromatographic methods, and the results from these two methods were almost identical. This assay should be used cautiously, if at all, in patients treated with other drugs that have potent anticholinergic effects. (Auth.)

  16. Qualitative research in finance

    OpenAIRE

    Dan Kaczynski; Michelle Salmona; Tom Smith

    2014-01-01

    This paper provides an overview of qualitative research to encourage finance researchers to apply a more diverse approach to current research practices. Social science researchers recognize that research questions should determine what research paradigm is best for each study. Imagine the benefits to finance if we expand our empirical sources of data to include what people have to say, which then allows us to explore the complex reasoning behind these conversations. It is the intent of this p...

  17. QSMSR QUALITATIVE MODEL

    OpenAIRE

    Tahir Abdullah; Shahbaz Nazeer

    2012-01-01

    Software architecture design and requirement engineering are core and independent areas of engineering. A lot of research, education and practice are carried on Requirement elicitation and doing refine it, but it is a major issue of engineering. QSMSR model act as a bridge between requirement and design there is a huge gap between these two areas of software architecture and requirement engineering. The QSMSR model divide into two sub model qualitative model and Principal model in this resear...

  18. Qualitative Psychology Nexus, Vol. I: Qualitative Research in Psychology

    OpenAIRE

    2004-01-01

    The first "Workshop Qualitative Research in Psychology" took place in Blaubeuren, Germany from October 20-22, 2000. The meeting was organized by the Center for Qualitative Psychology of the University of Tübingen, Germany. The purpose of the meeting was to begin a network of qualitative psychologists. Thirty-two participants got to know each other, presented and discussed their research, discussed potential further developments within the field of qualitative psychology, and inspired eac...

  19. Qualitative methodology in developmental psychology

    DEFF Research Database (Denmark)

    Demuth, Carolin; Mey, Günter

    2015-01-01

    Qualitative methodology presently is gaining increasing recognition in developmental psychology. Although the founders of developmental psychology to a large extent already used qualitative procedures, the field was long dominated by a (post) positivistic quantitative paradigm. The increasing...

  20. Database searches for qualitative research

    OpenAIRE

    Evans, David

    2002-01-01

    Interest in the role of qualitative research in evidence-based health care is growing. However, the methods currently used to identify quantitative research do not translate easily to qualitative research. This paper highlights some of the difficulties during searches of electronic databases for qualitative research. These difficulties relate to the descriptive nature of the titles used in some qualitative studies, the variable information provided in abstracts, and the differences in the ind...

  1. Comparison of several fixation methods for cytomegalovirus antigenemia assay.

    OpenAIRE

    Pérez, J.L.; De Oña, M; Niubò, J; Villar, H.; Melón, S.; García, A.; Martín, R.

    1995-01-01

    Two fixation methods based on formaldehyde or acetone for qualitative cytomegalovirus antigenemia assay were evaluated on 405 consecutive blood samples. Cytomegalovirus was detected in 40 samples by the antigenemia assay: 36 were detected by formaldehyde fixation; 22, by acetone; and 18, by both methods. Differences were statistically significant (P = 0.0043). In addition, four fixation methods (two based on formalin [with and without permeabilization] and two using acetone at different fixat...

  2. Qualitative Studies in Information Systems

    DEFF Research Database (Denmark)

    Sarker, Suprateek; Xiao, Xiao; Beaulieu, Tanya

    2013-01-01

    The authors discuss a review of qualitative papers on information systems (IS) published in various journals between 2001 and 2012. They explain trends related to qualitative research in the chosen journals and the key anatomical components of a qualitative research manuscript, including the...

  3. Detection of Giardia lamblia Antigens in Human Fecal Specimens by a Solid-Phase Qualitative Immunochromatographic Assay▿

    OpenAIRE

    Garcia, Lynne S.; Garcia, John Paul

    2006-01-01

    The SIMPLE-READ Giardia rapid assay (Medical Chemical Corporation) is a solid-phase qualitative immunochromatographic assay that detects Giardia lamblia in aqueous extracts of human fecal specimens. Testing 106 Giardia-positive and 104 Giardia-negative stool specimens yielded a sensitivity of 97.2% and a specificity of 100% for the SIMPLE-READ Giardia rapid assay.

  4. A Qualitative Report

    Directory of Open Access Journals (Sweden)

    Sara L. Ackerman

    2012-01-01

    Full Text Available Background. No in-depth qualitative research exists about the effects of therapeutic massage with children hospitalized to undergo hematopoietic cell transplantation (HCT. The objective of this study is to describe parent caregivers' experience of the effects of massage/acupressure for their children undergoing HCT. Methods. We conducted a qualitative analysis of open-ended interviews with 15 parents of children in the intervention arm of a massage/acupressure trial. Children received both practitioner and parent-provided massage/acupressure. Results. Parents reported that their child experienced relief from pain and nausea, relaxation, and greater ease falling asleep. They also reported increased caregiver competence and closeness with their child as a result of learning and performing massage/acupressure. Parents supported a semistandardized massage protocol. Conclusion. Massage/acupressure may support symptom relief and promote relaxation and sleep among pediatric HCT patients if administered with attention to individual patients' needs and hospital routines and may relieve stress among parents, improve caregiver competence, and enhance the sense of connection between parent and child.

  5. CPTAC Assay Portal: a repository of targeted proteomic assays

    Energy Technology Data Exchange (ETDEWEB)

    Whiteaker, Jeffrey R.; Halusa, Goran; Hoofnagle, Andrew N.; Sharma, Vagisha; MacLean, Brendan; Yan, Ping; Wrobel, John; Kennedy, Jacob; Mani, DR; Zimmerman, Lisa J.; Meyer, Matthew R.; Mesri, Mehdi; Rodriguez, Henry; Abbateillo, Susan E.; Boja, Emily; Carr, Steven A.; Chan, Daniel W.; Chen, Xian; Chen, Jing; Davies, Sherri; Ellis, Matthew; Fenyo, David; Hiltket, Tara; Ketchum, Karen; Kinsinger, Christopher; Kuhn, Eric; Liebler, Daniel; Lin, De; Liu, Tao; Loss, Michael; MacCoss, Michael; Qian, Weijun; Rivers, Robert; Rodland, Karin D.; Ruggles, Kelly; Scott, Mitchell; Smith, Richard D.; Thomas, Stefani N.; Townsend, Reid; Whiteley, Gordon; Wu, Chaochao; Zhang, Hui; Zhang, Zhen; Paulovich, Amanda G.

    2014-06-27

    To address these issues, the Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal (http://assays.cancer.gov) to serve as a public repository of well-characterized quantitative, MS-based, targeted proteomic assays. The purpose of the CPTAC Assay Portal is to facilitate widespread adoption of targeted MS assays by disseminating SOPs, reagents, and assay characterization data for highly characterized assays. A primary aim of the NCI-supported portal is to bring together clinicians or biologists and analytical chemists to answer hypothesis-driven questions using targeted, MS-based assays. Assay content is easily accessed through queries and filters, enabling investigators to find assays to proteins relevant to their areas of interest. Detailed characterization data are available for each assay, enabling researchers to evaluate assay performance prior to launching the assay in their own laboratory.

  6. Transgenic Animal Mutation Assays

    Institute of Scientific and Technical Information of China (English)

    Tao Chen; Ph.D.D.A.B.T.

    2005-01-01

    @@ The novel transgenic mouse and rat mutation assays have provided a tool for analyzing in vivo mutation in any tissue, thus permitting the direct comparison of cancer incidence with mutant frequency.

  7. The neutral comet assay

    International Nuclear Information System (INIS)

    The single-cell gel electrophoresis (or comet) assay is a rapid and sensitive fluorescence microscopic method that is used for the detection of DNA damage at the individual cell level. Comet assay under neutral conditions allows the detection of DNA double-strand breaks, considered to the biologically relevant radiation-induced lesion. In this report we describe modifications of the neutral comet method and its use for studying DNA damage and its repair in irradiated human lymphocytes. (author)

  8. The qualitative similarity hypothesis.

    Science.gov (United States)

    Paul, Peter V; Lee, Chongmin

    2010-01-01

    Evidence is presented for the qualitative similarity hypothesis (QSH) with respect to children and adolescents who are d/Deaf or hard of hearing. The primary focus is on the development of English language and literacy skills, and some information is provided on the acquisition of English as a second language. The QSH is briefly discussed within the purview of two groups of cognitive models: those that emphasize the cognitive development of individuals and those that pertain to disciplinary or knowledge structures. It is argued that the QSH has scientific merit with implications for classroom instruction. Future research should examine the validity of the QSH in other disciplines such as mathematics and science and should include perspectives from social as well as cognitive models. PMID:20415280

  9. Qualitative research, tourism

    DEFF Research Database (Denmark)

    Ren, Carina Bregnholm

    2016-01-01

    various methods, which seek to deploy more inductive and explorative approaches. Such methods include interviews, participant or non-participatory observations, focus groups, text and discourse analysis, photo and video documentation or elicitation, semiotic studies, autoethnography, and virtual......) and a drastic rise in qualitative research based publications in prominent journals such as Annals of Tourism Research and, to a lesser degree, Tourism Management. Tourism as a heterogeneous field Since the turn of the century, the research community is progressively coming to terms with the contributions...... with which to study tourism not only as an industry and business, but also as a mundane, embodied, enacted, and localized practice. Since then, a focus on mobility, affect, emotions, materiality, everyday life, and performativity has increased immensely and shown how cultural or social issues...

  10. Qualitative experiments in psychology

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2015-01-01

    In this article, I explore the meaning of experiments in early twentieth century psychology, focusing on the qualitative experimental methodology of psychologist Frederic BARTLETT. I begin by contextualizing BARTLETT's experiments within the continental research tradition of his time, which...... was in a state of transition from a focus on elements (the concern of psychophysics) to a focus on wholes (the concern of Gestalt psychology). The defining feature of BARTLETT's early experiments is his holistic treatment of human responses, in which the basic unit of analysis is the active person relating...... to some material within the constraints of a social and material context. This manifests itself in a number of methodological principles that contrast with contemporary understandings of experimentation in psychology. The contrast is further explored by reviewing the history of "replications...

  11. Ethics and the practice of qualitative research

    DEFF Research Database (Denmark)

    Shaw, Ian Frank

    2016-01-01

    Ethics and the practice of qualitative research? Qualitative Social Work 7 (4): 400-414. Reprinted......Ethics and the practice of qualitative research? Qualitative Social Work 7 (4): 400-414. Reprinted...

  12. Identification of irradiated pepper with comet assay

    International Nuclear Information System (INIS)

    The treatment of foods with ionizing radiations is a technological process utilized in order to increase the hygienic quality and the storage time of the foods. Several methods of detection of irradiated foods have been recommended. The comet assay of DNA is one fast and economical technique for the qualitative identification of irradiated foods. The objective of the present paper was to identify with the comet assay technique the modifications of the DNA molecule of irradiated pepper storage at environment and refrigeration temperatures and different post-irradiation times for different absorbed dose values, (0.1, 0.3 and 0.5 kGy). It was demonstrated that for the high absorbed dose values was observed a greater break into fragments of the DNA molecule, which shows the application of this technique for the identification of irradiated foods. (author)

  13. Identification of irradiated pepper with comet assay

    Energy Technology Data Exchange (ETDEWEB)

    Prieto Miranda, Enrique Fco.; Moreno Alvarez, Damaris L.; Carro Palacio, Sandra [Centro de Aplicaciones Tecnologicas y Desarrollo Nuclear. (CEADEN), Ciudad de La Habana (Cuba)]. E-mail: efprieto@ceaden.edu.cu; damaris@ceaden.edu.cu; Iglesia Enriquez, Isora [Instituto de Investigacion para la Industria Alimenticia (IIIA), Ciudad de La Habana (Cuba)

    2007-07-01

    The treatment of foods with ionizing radiations is a technological process utilized in order to increase the hygienic quality and the storage time of the foods. Several methods of detection of irradiated foods have been recommended. The comet assay of DNA is one fast and economical technique for the qualitative identification of irradiated foods. The objective of the present paper was to identify with the comet assay technique the modifications of the DNA molecule of irradiated pepper storage at environment and refrigeration temperatures and different post-irradiation times for different absorbed dose values, (0.1, 0.3 and 0.5 kGy). It was demonstrated that for the high absorbed dose values was observed a greater break into fragments of the DNA molecule, which shows the application of this technique for the identification of irradiated foods. (author)

  14. Presenting and Evaluating Qualitative Research

    OpenAIRE

    Anderson, Claire

    2010-01-01

    The purpose of this paper is to help authors to think about ways to present qualitative research papers in the American Journal of Pharmaceutical Education. It also discusses methods for reviewers to assess the rigour, quality, and usefulness of qualitative research. Examples of different ways to present data from interviews, observations, and focus groups are included. The paper concludes with guidance for publishing qualitative research and a checklist for authors and reviewers.

  15. Common Perspectives in Qualitative Research.

    Science.gov (United States)

    Flannery, Marie

    2016-07-01

    The primary purpose of this column is to focus on several common core concepts that are foundational to qualitative research. Discussion of these concepts is at an introductory level and is designed to raise awareness and understanding of several conceptual foundations that undergird qualitative research. Because of the variety of qualitative approaches, not all concepts are relevant to every design and tradition. However, foundational aspects were selected for highlighting. PMID:27314194

  16. Qualitative research in ophthalmic sciences

    OpenAIRE

    Murthy G; Gupta Sanjeev

    1999-01-01

    Qualitative research was the preserve of social scientists before making inroads into medical sciences, as the medical fraternity was wedded to evidence-based medicine. Qualitative research methods are now being increasingly used in medicine. The goal of qualitative research is to help in the understanding of social phenomenon in their natural settings. These methods help to unravel mysteries relating to complex human interactions and to study the role of human behaviour in health and disease...

  17. Qualitative Equations: The Confluence Case

    OpenAIRE

    Aubin, J.-P.

    1988-01-01

    This paper deals with a domain of Artificial Intelligence known under the name of "qualitative simulation" or "qualitative physics", to which special volumes of "Artificial Intelligence" (1984) and or "IEEE Transactions on Systems, Man and Cybernetics" (1987) have been devoted. It defines the concept of "qualitative frame" of a set, which allows to introduce strict, large and dual confluence frames of a finite dimensional vector-space. After providing a rigorous definition of standa...

  18. Qualitative methods in travel surveys

    OpenAIRE

    HANNES, Els; Janssens, Davy; Wets, Geert

    2009-01-01

    This chapter explains the added value of integrating qualitative methods in travel surveys. The introduction briefly frames some general aspects of qualitative research. Distinct steps in a research process structure the remainder of this chapter. First, questions and contexts most applicable for qualitative methods are addressed. Next, its actual use, role and timing in travel surveys are discussed. Sampling and data collection issues are treated subsequently, followed by a brief account on ...

  19. Assay method and compositions

    International Nuclear Information System (INIS)

    Methods are described for measuring catecholamine levels in human and animal body fluids and tissues using the catechol-O-methyl-transferase (COMT) radioassay. The assay involves incubating the biological sample with COMT and the tritiated methyl donor, S-adenosyl-L-methionine(3H)-methyl. The O-methylated (3H) epinephrine and/or norepinephrine are extracted and oxidised to vanillin-3H which in turn is extracted and its radioactivity counted. When analysing dopamine levels the assay is extended by vanillin-3H and raising the pH of the aqueous periodate phase from which O-methylated (3H) dopamine is extracted and counted. The assay may be modified depending on whether measurements of undifferentiated total endogenous catecholamine levels or differential analyses of the catecholamine levels are being performed. The sensitivity of the assay can be as low as 5 picograms for norepinephrine and epinephrine and 12 picograms for dopamine. The assemblance of the essential components of the assay into a kit for use in laboratories is also described. (U.K.)

  20. Rover waste assay system

    Energy Technology Data Exchange (ETDEWEB)

    Akers, D.W.; Stoots, C.M.; Kraft, N.C.; Marts, D.J. [Idaho National Engineering Lab., Idaho Falls, ID (United States)

    1997-11-01

    The Rover Waste Assay System (RWAS) is a nondestructive assay system designed for the rapid assay of highly-enriched {sup 235}U contaminated piping, tank sections, and debris from the Rover nuclear rocket fuel processing facility at the Idaho Chemical Processing Plant. A scanning system translates a NaI(Tl) detector/collimator system over the structural components where both relative and calibrated measurements for {sup 137}Cs are made. Uranium-235 concentrations are in operation and is sufficiently automated that most functions are performed by the computer system. These functions include system calibration, problem identification, collimator control, data analysis, and reporting. Calibration of the system was done through a combination of measurements on calibration standards and benchmarked modeling. A description of the system is presented along with the methods and uncertainties associated with the calibration and analysis of the system for components from the Rover facility. 4 refs., 2 figs., 4 tabs.

  1. Rover waste assay system

    International Nuclear Information System (INIS)

    The Rover Waste Assay System (RWAS) is a nondestructive assay system designed for the rapid assay of highly-enriched 235U contaminated piping, tank sections, and debris from the Rover nuclear rocket fuel processing facility at the Idaho Chemical Processing Plant. A scanning system translates a NaI(Tl) detector/collimator system over the structural components where both relative and calibrated measurements for 137Cs are made. Uranium-235 concentrations are in operation and is sufficiently automated that most functions are performed by the computer system. These functions include system calibration, problem identification, collimator control, data analysis, and reporting. Calibration of the system was done through a combination of measurements on calibration standards and benchmarked modeling. A description of the system is presented along with the methods and uncertainties associated with the calibration and analysis of the system for components from the Rover facility. 4 refs., 2 figs., 4 tabs

  2. Neutral Comet Assay

    OpenAIRE

    2013-01-01

    The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding dye. Damaged DNA (charged DNA) migrates in this field, forming the tail of a “comet”, while undamaged DNA remained in the head of the “comet”. The ...

  3. Lateral flow strip assay

    Science.gov (United States)

    Miles, Robin R.; Benett, William J.; Coleman, Matthew A.; Pearson, Francesca S.; Nasarabadi, Shanavaz L.

    2011-03-08

    A lateral flow strip assay apparatus comprising a housing; a lateral flow strip in the housing, the lateral flow strip having a receiving portion; a sample collection unit; and a reagent reservoir. Saliva and/or buccal cells are collected from an individual using the sample collection unit. The sample collection unit is immersed in the reagent reservoir. The tip of the lateral flow strip is immersed in the reservoir and the reagent/sample mixture wicks up into the lateral flow strip to perform the assay.

  4. Automated phantom assay system

    International Nuclear Information System (INIS)

    This paper describes an automated phantom assay system developed for assaying phantoms spiked with minute quantities of radionuclides. The system includes a computer-controlled linear-translation table that positions the phantom at exact distances from a spectrometer. A multichannel analyzer (MCA) interfaces with a computer to collect gamma spectral data. Signals transmitted between the controller and MCA synchronize data collection and phantom positioning. Measured data are then stored on disk for subsequent analysis. The automated system allows continuous unattended operation and ensures reproducible results

  5. Qualitative Analysis of Somitogenesis Models

    Directory of Open Access Journals (Sweden)

    Maschke-Dutz E.

    2007-12-01

    Full Text Available Although recently the properties of a single somite cell oscillator have been intensively investigated, the system-level nature of the segmentation clock remains largely unknown. To elaborate qualitatively this question, we examine the possibility to transform a well-known time delay somite cell oscillator to dynamical system of differential equations allowing qualitative analysis.

  6. Quality assurance of qualitative analysis

    DEFF Research Database (Denmark)

    Ríos, Ángel; Barceló, Damiá; Buydens, Lutgarde;

    2003-01-01

    The European Commission has supported the G6MA-CT-2000-01012 project on "Metrology of Qualitative Chemical Analysis" (MEQUALAN), which was developed during 2000-2002. The final result is a document produced by a group of scientists with expertise in different areas of chemical analysis, metrology......: traceability, reliability (uncertainty), validation, and internal/external quality control for qualitative methods....

  7. Using Numbers in Qualitative Research

    Science.gov (United States)

    Maxwell, Joseph A.

    2010-01-01

    The use of numerical/quantitative data in qualitative research studies and reports has been controversial. Prominent qualitative researchers such as Howard Becker and Martyn Hammersley have supported the inclusion of what Becker called "quasi-statistics": simple counts of things to make statements such as "some," "usually," and "most" more…

  8. Hyaluronic Acid Assays

    DEFF Research Database (Denmark)

    Itenov, Theis S; Kirkby, Nikolai S; Bestle, Morten H;

    2015-01-01

    BACKGROUD: Hyaluronic acid (HA) is proposed as a marker of functional liver capacity. The aim of the present study was to compare a new turbidimetric assay for measuring HA with the current standard method. METHODS: HA was measured by a particle-enhanced turbidimetric immunoassay (PETIA) and enzyme...

  9. Instrument for assaying radiation

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Jody Rustyn; Farfan, Eduardo B.

    2016-03-22

    An instrument for assaying radiation includes a flat panel detector having a first side opposed to a second side. A collimated aperture covers at least a portion of the first side of the flat panel detector. At least one of a display screen or a radiation shield may cover at least a portion of the second side of the flat panel detector.

  10. Multiplex, Quantitative, Real-Time PCR Assay for Cytomegalovirus and Human DNA

    OpenAIRE

    Sanchez, Jason L.; Storch, Gregory A.

    2002-01-01

    We created a multiplex, quantitative, real-time PCR assay that amplifies cytomegalovirus (CMV) and human DNA in the same reaction tube, allowing for a viral load determination that is normalized to measured human DNA. The assay targets a conserved region of the CMV DNA polymerase gene that is not affected by known drug resistance mutations. All 36 strains of CMV detected by culture or qualitative PCR in a population of lung transplant recipients were detected. The assay detected 1 to 10 copie...

  11. Positivism and qualitative nursing research.

    Science.gov (United States)

    Paley, J

    2001-01-01

    Despite the hostility to positivism shown by qualitative methodologists in nursing, as in other disciplines, the epistemological and ontological instincts of qualitative researchers seem to coincide with those of the positivists, especially Bayesian positivists. This article suggests that positivists and qualitative researchers alike are pro-observation, proinduction, pro-plausibility and pro-subjectivity. They are also anti-cause, anti-realist, anti-explanation, anti-correspondence, anti-truth. In only one respect is there a significant difference between positivist and qualitative methodologists: most positivists have believed that, methodologically, the natural sciences and the social sciences are the same; most qualitative researchers are adamant that they are not. However, if positivism fails as a philosophy of the natural sciences (which it probably does), it might well succeed as a philosophy of the social sciences, just because there is a methodological watershed between the two. Reflex antagonism to positivism might therefore be a major obstacle to understanding the real reasons why qualitative research and the natural sciences are methodologically divergent; and less hostility on the part of qualitative nurse researchers might bring certain advantages in its wake. PMID:11885869

  12. Qualitative Psycholgy Nexus Vol. VIII: Epistemologies for Qualitative Research

    OpenAIRE

    Soini, Hannu; Kronqvist, Eeva-Liisa; Huber, Günter L.; Maxwell, Joseph; Kiegelmann, Mechthild; Gento, Samuel; Medina, Antonio; Pina, Jorge; Domínguez, María Concepción; Schweizer, Karin; Korte, Petra; Pavlovic, Jelena; Suorsa, Teemu; Feliz, Sálvora; Feliz, Tiberio

    2011-01-01

    The 9th annual meeting of the Center for Qualitative Psychology was held in Northern Finland, near to the city of Oulu. The seminar was started with two crucial questions leading the participants to the theme of the conference. The orientation followed the line of the former meetings of the Center for Qualitative Research in Psychology helping participants to reflect own ways of doing psychological research. In the beginning of the conference we asked the conference participant...

  13. Dual isotope assays

    International Nuclear Information System (INIS)

    Dual isotope assays for thyroid function are performed by carrying out a radio-immunoassay for two of thyroxine (T4), tri-iodothyronine (T3), thyroid stimulating hormone (TSH), and thyroxine binding globulin (TBG), by a method wherein a version of one of the thyroid components, preferably T4 or T3 is labelled with Selenium-75 and the version of the other thyroid component is labelled with a different radionuclide, preferably Iodine-125. (author)

  14. Automated uranium assays

    International Nuclear Information System (INIS)

    Precise, timely inventories of enriched uranium stocks are vital to help prevent the loss, theft, or diversion of this material for illicit use. A wet-chemistry analyzer has been developed at LLL to assist in these inventories by performing automated analyses of uranium samples from different stages in the nuclear fuel cycle. These assays offer improved accuracy, reduced costs, significant savings in manpower, and lower radiation exposure for personnel compared with present techniques

  15. Real-Time PCR Assay Compared to Nested PCR and Antigenemia Assays for Detecting Cytomegalovirus Reactivation in Adult T-Cell Leukemia-Lymphoma Patients

    OpenAIRE

    Ikewaki, Junji; Ohtsuka, Eiichi; Kawano, Rie; Ogata, Masao; Kikuchi, Hiroshi; Nasu, Masaru

    2003-01-01

    We analyzed the efficiency of the quantitative real-time PCR assay for cytomegalovirus (CMV) reactivation in adult T-cell leukemia-lymphoma (ATL) patients and compared the results with those obtained with qualitative nested PCR and antigenemia assays. The viral load obtained by the real-time PCR assay closely paralleled the number of antigen-positive cells obtained with the antigenemia assay. Real-time PCR revealed that a large number of DNA copies could be present even in samples assessed as...

  16. Disagreement between Human Papillomavirus Assays

    DEFF Research Database (Denmark)

    Rebolj, Matejka; Preisler, Sarah; Ejegod, Ditte Møller;

    2014-01-01

    We aimed to determine the disagreement in primary cervical screening between four human papillomavirus assays: Hybrid Capture 2, cobas, CLART, and APTIMA. Material from 5,064 SurePath samples of women participating in routine cervical screening in Copenhagen, Denmark, was tested with the four...... assays. Positive agreement between the assays was measured as the conditional probability that the results of all compared assays were positive given that at least one assay returned a positive result. Of all 5,064 samples, 1,679 (33.2%) tested positive on at least one of the assays. Among these, 41......-65 years (n = 2,881), 23% tested positive on at least one assay, and 42 to 58% of these showed positive agreement on any compared pair of the assays. While 4% of primary screening samples showed abnormal cytology, 6 to 10% were discordant on any pair of assays. A literature review corroborated our findings...

  17. Qualitative Capacities as Imprecise Possibilities.

    OpenAIRE

    Dubois, Didier; Prade, Henri; Rico, Agnés

    2013-01-01

    National audience This paper studies the structure of qualitative capacities, that is, monotonic set-functions, when they range on a finite totally ordered scale equipped with an order-reversing map. These set-functions correspond to general representations of uncertainty, as well as importance levels of groups of criteria in multicriteria decision-making. More specifically, we investigate the question whether these qualitative set-functions can be viewed as classes of simpler set-function...

  18. Designs for qualitatively orientated research

    OpenAIRE

    Philipp Mayring

    2007-01-01

    It is shown that contemporary psychology appears to be hindered by its orientation to natural science and experimental methodology. An opening to more combinations of methods, to an integration of qualitative and quantitative approaches is needed. Several foundations for such an integration (pragmatism, constructivism, dialectics) are given. An integration is only possible if qualitatively orientated studies have a clear conception, a study design, and a research process model which fits into...

  19. Qualitative methods : the new orthodoxy?

    OpenAIRE

    Crang, M.

    2002-01-01

    It is an interesting moment to write Progress in Human Geography’s first report on qualitative methods. In one sense, it suggests these methods have, at long last, arrived and been accepted as established approaches. That this is an overdue recognition needs little emphasizing when surveying the number of articles drawing upon, at least in part, qualitative material. However, a less encouraging omen is the recent column in the UK Economic and Social Research Council’s Social Sciences, penned ...

  20. Qualitative Data Mining and Its Applications

    OpenAIRE

    Bratko, Ivan; Šuc, Dorian

    2003-01-01

    In machine learning from numerical data, usually the target concept is a numerical function that facilitates quantitative prediction. In contrast to this, we consider qualitative data mining which aims at finding qualitative patterns, or qualitative relationships in numerical data. We present one approach to qualitative data mining, in which the target concepts are expressed as qualitative decision trees. We reviewsome case studies in qualitative data mining, and discuss typical application s...

  1. Use of laminar flow patterning for miniaturised biochemical assays

    DEFF Research Database (Denmark)

    Regenberg, Birgitte; Krühne, Ulrich; Beyer, M.;

    2004-01-01

    Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive...... species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 mm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor-ligand assays, recording biotin...

  2. Radiorespirometic assay device

    International Nuclear Information System (INIS)

    A radiorespirometic assay device is described in which the presence of microorganisms in a sample is determined by placing the sample in contact with a metabolisable radioactive labelled substrate, collecting any gas evolved, exposing a photosensitive material to the gas and determining if a spot is produced on the material. A spot indicates the presence of radioactivity showing that the substrate has been metabolized by a microorganism. Bacteria may be detected in body fluids, hospital operating rooms, water, food, cosmetics and drugs. (U.K.)

  3. Radon assay for SNO+

    International Nuclear Information System (INIS)

    The SNO+ experiment will study neutrinos while located 6,800 feet below the surface of the earth at SNOLAB. Though shielded from surface backgrounds, emanation of radon radioisotopes from the surrounding rock leads to back-grounds. The characteristic decay of radon and its daughters allows for an alpha detection technique to count the amount of Rn-222 atoms collected. Traps can collect Rn-222 from various positions and materials, including an assay skid that will collect Rn-222 from the organic liquid scintillator used to detect interactions within SNO+

  4. Radon assay for SNO+

    Energy Technology Data Exchange (ETDEWEB)

    Rumleskie, Janet [Laurentian University, Greater Sudbury, Ontario (Canada)

    2015-12-31

    The SNO+ experiment will study neutrinos while located 6,800 feet below the surface of the earth at SNOLAB. Though shielded from surface backgrounds, emanation of radon radioisotopes from the surrounding rock leads to back-grounds. The characteristic decay of radon and its daughters allows for an alpha detection technique to count the amount of Rn-222 atoms collected. Traps can collect Rn-222 from various positions and materials, including an assay skid that will collect Rn-222 from the organic liquid scintillator used to detect interactions within SNO+.

  5. Canberra waste assay systems

    International Nuclear Information System (INIS)

    Canberra Industries, Inc. (USA) offers complete solutions and turnkey systems for qualifying and quantifying drums of radioactive waste. Different Waste Assay Systems are available for low-, medium-, and high activity barrels. Such systems scan the segments of the drums. The gamma-emitting nuclides present in the radioactive waste are identified from the spectra of the gamma-rays emitted and their activities are quantified. The spectrum of each segment of the waste container is analysed separately and the results are summed for total drum inventory reporting. The Waste Assay specific Canberra software is an easy-to-use menu driven program. It offers automatic procedures for routine work but interactive mode is also available to investigate particular cases. The way of analysis and the format and contents of the report can be customized. Additional Quality Control program module monitors system parameters. The measured and calculated values of each waste container are saved into a relational database. Canberra offers mathematical efficiency calibration services for different drum types, with different waste densities and different activities. This new theoretical approach of efficiency calibration eliminates the need of calibration drums, thus saving costs. For field applications Canberra developed a portable In-Situ Object Counting System. This system determines efficiency calibration for any kind of objects after the user enters its size and density, furthermore the type of shielding and the distance between the detector and the object. (author)

  6. The Epistemology of Qualitative Research

    Directory of Open Access Journals (Sweden)

    Howard S. Becker

    2014-07-01

    Full Text Available This article discusses questions that are relevant to the epistemology of qualitative research. In order to do so, the presumed dichotomy between qualitative and quantitative research is discussed and challenged. According to the author, the similarities between these methods are more relevant than its differences. Both methods strive to describe the social reality and thus have the same epistemological basis, even though they emphasize different questions. To shed light in such dichotomy, the author explores the origins of epistemology as a discipline and its philosophical character. Finally, the particularities and advantages of qualitative research are discussed, especially ethnography and field research, through an analysis of some of its main aspects for observing social reality: its focus on the point of view of the actor; the observation of the everyday world and the full and thick description. 

  7. Qualitative Methodology in Unfamiliar Cultures

    DEFF Research Database (Denmark)

    Svensson, Christian Franklin

    2014-01-01

    This case study discusses qualitative fieldwork in Malaysia. The trends in higher education led to investigating how and why young Indians and Chinese in Malaysia are using the university to pursue a life strategy. Given the importance of field context in designing and analysing research based on a...... qualitative methodology, conscious reflection on research design and objectivity is important when doing fieldwork. This case study discusses such reflections. Emphasis throughout is given to applied qualitative methodology and its contributions to the social sciences, in particular having to do with...... relational, emotional, and ethical issues associated with interviewing and personal observation. Although the empirical setting of this case is Southeast Asia, the various discussions and interrelatedness of methodology, theory, and empirical reflections will prove applicable to field studies throughout the...

  8. Collective Analysis of Qualitative Data

    DEFF Research Database (Denmark)

    Simonsen, Jesper; Friberg, Karin

    2014-01-01

    What. Many students and practitioners do not know how to systematically process qualitative data once it is gathered—at least not as a collective effort. This chapter presents two workshop techniques, affinity diagramming and diagnostic mapping, that support collective analysis of large amounts of...... qualitative data. Affinity diagramming is used to make collective analysis and interpretations of qualitative data to identify core problems that need to be addressed in the design process. Diagnostic mapping supports collective interpretation and description of these problems and how to intervene in them. We...... explain the techniques through a case where they were used to analyze why a new elec- tronic medical record system introduced life-threatening situations for patients. Why. Collective analyses offer all participants a voice, visualize their contributions, combine different actors’ perspectives, and anchor...

  9. FLUIDICS DEVICE FOR ASSAY

    DEFF Research Database (Denmark)

    2007-01-01

    The present invention relates to a device for use in performing assays on standard laboratory solid supports whereon chemical entities are attached. The invention furthermore relates to the use of such a device and a kit comprising such a device. The device according to the present invention is...... adapted to receive one or more replaceable solid support(s) (40) onto which chemical entities (41) are attached, said device comprising a base (1, 60, 80, 300, 400, 10, 70, 140, 20, 90, 120, 150, 30, 100), one or more inlet(s) (5), one or more outlet(s) (6). The base and the solid support (40) defines......, when operatively connected, one or more chambers (21) comprising the chemical entities (41), the inlet(s) (5) and outlet(s) (6) and chambers (21) being in fluid connection. The device further comprise means for providing differing chemical conditions in each chamber (21)....

  10. Assay of oestrogen

    International Nuclear Information System (INIS)

    A particular problem with the direct radioimmunoassay of unconjugated oestriol in pregnancy is caused by the increased amount of steroid-binding proteins present in pregnancy serum and plasma. The steroid-binding proteins react with oestriol and 125I-labelled oestriol during the assay procedure and the steroid-protein bound 125I-labelled oestriol is precipitated along with the antibody-bound 125I-labelled oestriol by the ammonium sulphate solution separation system. A novel method is described whereby progesterone (1-20 μg/ml) is used to block the action of steroid-binding proteins in pregnancy serum and plasma samples, thus minimizing interference in a direct radioimmunoassay for unconjugated oestriol using a specific anti-oestriol serum. (U.K.)

  11. Qualitative Inquiry in Everyday Life

    DEFF Research Database (Denmark)

    Brinkmann, Svend

    This book is a 'survival guide' for students and researchers who would like to conduct a qualitative study with limited resources. Brinkmann shows how everyday life materials such as books, television, the internet, the media and everyday conversations and interactions can help us to understand...... helps its reader develop a disciplined and analytic awareness informed by theory, and shows how less can be more in qualitative research. Each chapter introduces theoretical tools to think with, and demonstrates how they can be put to use in working concretely with everyday life materials....

  12. Qualitative methods in theoretical physics

    CERN Document Server

    Maslov, Dmitrii

    2016-01-01

    This book comprises a set of tools which allow researchers and students to arrive at a qualitatively correct answer without undertaking lengthy calculations. In general, Qualitative Methods in Theoretical Physics is about combining approximate mathematical methods with fundamental principles of physics: conservation laws and symmetries. Readers will learn how to simplify problems, how to estimate results, and how to apply symmetry arguments and conduct dimensional analysis. A comprehensive problem set is included. The book will appeal to a wide range of students and researchers.

  13. Qualitative Research Articles: Guidelines, Suggestions and Needs

    Science.gov (United States)

    Crescentini, Alberto; Mainardi, Giuditta

    2009-01-01

    Purpose: The purpose of this paper is to give ideas and suggestions to avoid some typical problems of qualitative articles. The aim is not to debate quality in qualitative research but to indicate some practical solutions. Design/methodology/approach: The paper discusses the design of qualitative research and the structure of a qualitative article…

  14. Qualitative computing and qualitative research: addressing the challenges of technology and globalization

    OpenAIRE

    Cisneros Puebla, César A.; Davidson, Judith

    2012-01-01

    "Qualitative computing has been part of our lives for thirty years. Today, we urgently call for an evaluation of its international impact on qualitative research. Evaluating the international impact of qualitative research and qualitative computing requires a consideration of the vast amount of qualitative research over the last decades, as well as thoughtfulness about the uneven and unequal way in which qualitative research and qualitative computing are present in different fields of study a...

  15. PathogenMip Assay: A Multiplex Pathogen Detection Assay

    OpenAIRE

    Akhras, Michael S.; Sreedevi Thiyagarajan; Villablanca, Andrea C.; Davis, Ronald W; Pål Nyrén; Nader Pourmand

    2007-01-01

    The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical cancer progression. Probe construction was based on a novel, cost-effective, ligase-based protocol. The ...

  16. Qualitative Methods Used in Market Research

    OpenAIRE

    Gabriel Bratucu; Tamara-Oana Bratucu

    2006-01-01

    According to the given data, admittedly, there are two main types of market research: Quantitative Research (questionnaire surveys) and Qualitative Research. Qualitative Research implies getting answers from a small number of respondents and the results of qualitative nature: ideas, perceptions, attitudes, associated values, preferences, suggestions. The Qualitative Research is conducted in the initial phase of studying one issue. The main purpose of Qualitative Research is to define the natu...

  17. Reconsidering Constructivism in Qualitative Research

    Science.gov (United States)

    Lee, Cheu-Jey George

    2012-01-01

    This article examines constructivism, a paradigm in qualitative research that has been propagated by Egon Guba, Yvonna Lincoln, and Norman Denzin. A distinction is made between whether the basic presuppositions of constructivism are credible compared to those of a competing paradigm and whether constructivism's beliefs are internally consistent.…

  18. Caregiving: A Qualitative Concept Analysis

    Science.gov (United States)

    Hermanns, Melinda; Mastel-Smith, Beth

    2012-01-01

    A common definition of caregiving does not exist. In an attempt to define the concept of caregiving, the authors used a hybrid qualitative model of concept development to analyze caregiving. The model consists of three phases: (a) theoretical, (b) fieldwork, and (c) analytical. The theoretical phase involves conducting an interdisciplinary…

  19. Qualitative Research in Rehabilitation Counseling

    Science.gov (United States)

    Hanley-Maxwell, Cheryl; Al Hano, Ibrahim; Skivington, Michael

    2007-01-01

    Qualitative research approaches offer rehabilitation scholars and practitioners avenues into understanding the lives and experiences of people with disabilities and those people and systems with whom they interact. The methods used often parallel those used in counseling and appear to be well matched with the field of rehabilitation counseling.…

  20. Philosophical foundations of qualitative research.

    Science.gov (United States)

    Boyd, C O

    1993-08-01

    Although new and still emerging for us, qualitative research approaches have been receiving considerable attention for some time in other disciplines. Along with philosophical debates, there are debates about whether there needs to be a debate. On a philosophical level, there is irreconcilable conflict between the quantitative and qualitative paradigms. It is important to recognize this conflict, avoiding illogical compromise. Yet, proponents of each paradigm need to applaud both the existence of the other and the hybrid paradigms that inevitably are born of conflict. An apt beginning would be broader definitions of what constitutes science and research in nursing, eliminating the sense-organ bias that is so contrary to our philosophy for practice. This alone would provide qualitative nurse researchers with the sanction they need to progress in their exploration of various approaches to creating a science and a body of knowledge in, for, and about nursing practice. In the chapters to follow, readers will be introduced to several qualitative research approaches. Each approach represents an interpretation of the qualitative paradigm in nursing research, grounded in the general perspective of phenomenological philosophy. This perspective focuses on phenomena as they appear and recognizes that reality is subjective and a matter of appearances for us in our social world. Subjectivity means that the world becomes real through our contact with it and acquires meaning through our interpretations of that contact. Truth, then, is a composite of realities, and access to truth is a problem of access to human subjectivity. This perspective guides the qualitative researcher in nursing to the subject matter of lived experiences, which are the original contacts with a world, and of the processes and content of interpretation--the meaning attributions that constitute realities and perspectives for a future of possibilities in the world. Other consequences of a phenomenological

  1. Experience of fibromyalgia. Qualitative study.

    OpenAIRE

    Raymond, M. C.; Brown, J. B.

    2000-01-01

    OBJECTIVE: To explore illness experiences of patients diagnosed with fibromyalgia. DESIGN: Qualitative method of in-depth interviews. SETTING: Midsize city in Ontario. PARTICIPANTS: Seven patients diagnosed with fibromyalgia. METHOD: Seven in-depth interviews were conducted to explore the illness experience of patients diagnosed with fibromyalgia. All interviews were audiotaped and transcribed verbatim. All interview transcriptions were read independently by the researchers, who then compared...

  2. Qualitative theory of rubber friction

    OpenAIRE

    Persson, B.N.J.; E. Tosatti

    2000-01-01

    When rubber is slid on a hard, rough substrate, the surface asperities of the substrate exert oscillating forces on the rubber surface leading to energy "dissipation" via the internal friction of the rubber. We present a qualitative discussion of how the resulting friction force depends on the nature of the surface roughness and comment on the origin of the wear of sliding rubber surfaces. (C) 2000 American Institute of Physics. [S0021-9606(00)51404-4].

  3. Qualitative Psychology Nexus, Vol. II: The Role of the Researcher in Qualitative Psychology

    OpenAIRE

    2002-01-01

    Volume two of Qualitative Research Nexus focuses on the roles of qualitative researchers and their relationships within psychological studies. This book is a result of the presentations, discussions, and collaborations of participants at the second workshop "Qualitative Psychology" in October 2001 in Blaubeuren, Germany that was organized by the Center for Qualitative Psychology. The theme of the meeting was "the role of the researcher in qualitative psychology." Reading this volume of Qualit...

  4. Dye Labelled Monoclonal Antibody Assay for Detection of Toxic Shock Syndrome Toxin -1 from Staphylococcus Aureus

    Directory of Open Access Journals (Sweden)

    V Javid Khojasteh

    2011-12-01

    Full Text Available Objective: The aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (DLMAA, using non-radioactive organic synthetic dyes for identification of Toxic Shock Syndrome Toxin-1 (TSST-1 producing strains of Staphylococcus aureus.Materials and Methods: The assay protocol required only two simple steps; addition of TSST-1 antigen to a nitrocellulose membrane and then adding a colloidal dye labelled antibody (D/A suspension detection reagent.Results: The sensitivity and specificity of the assay was determined relative to positive and negative strains compared to an ELISA assay. Overall 100% agreement was found between both assays. The sensitivity for detection of TSST-1 was 30 ng.Conclusion: The DLMAA did not require handling and disposal of radioactive materials. It is a rapid qualitative technique for detection of TSST-1 toxin at room temperature within a short time.

  5. From Antenna to Assay

    Science.gov (United States)

    Moore, Evan G.; Samuel, Amanda P. S.; Raymond, Kenneth N.

    2009-01-01

    Conspectus Ligand-sensitized, luminescent lanthanide(III) complexes are of considerable importance because their unique photophysical properties (microsecond to millisecond lifetimes, characteristic and narrow emission bands, and large Stokes shifts) make them well suited as labels in fluorescence-based bioassays. The long-lived emission of lanthanide(III) cations can be temporally resolved from scattered light and background fluorescence to vastly enhance measurement sensitivity. One challenge in this field is the design of sensitizing ligands that provide highly emissive complexes with sufficient stability and aqueous solubility for practical applications. In this Account, we give an overview of some of the general properties of the trivalent lanthanides and follow with a summary of advances made in our laboratory in the development of highly luminescent Tb(III) and Eu(III) complexes for applications in biotechnology. A focus of our research has been the optimization of these compounds as potential commercial agents for use in Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our approach involves developing high-stability octadentate Tb(III) and Eu(III) complexes that rely on all-oxygen donor atoms and using multi-chromophore chelates to increase molar absorptivity; earlier examples utilized a single pendant chromophore (that is, a single “antenna”). Ligands based on 2-hydroxyisophthalamide (IAM) provide exceptionally emissive Tb(III) complexes with quantum yield values up to ∼60% that are stable at the nanomolar concentrations required for commercial assays. Through synthetic modification of the IAM chromophore and time-dependent density functional theory (TD-DFT) calculations, we have developed a method to predict absorption and emission properties of these chromophores as a tool to guide ligand design. Additionally, we have investigated chiral IAM ligands that yield Tb(III) complexes possessing both high quantum yield values and strong

  6. Quantifying Qualitative Data Using Cognitive Maps

    Science.gov (United States)

    Scherp, Hans-Ake

    2013-01-01

    The aim of the article is to show how substantial qualitative material consisting of graphic cognitive maps can be analysed by using digital CmapTools, Excel and SPSS. Evidence is provided of how qualitative and quantitative methods can be combined in educational research by transforming qualitative data into quantitative data to facilitate…

  7. Strategies of Qualitative Inquiry. Third Edition

    Science.gov (United States)

    Denzin, Norman K., Ed.; Lincoln, Yvonna S., Ed.

    2007-01-01

    "Strategies of Qualitative Inquiry, Third Edition," the second volume in the paperback version of "The SAGE Handbook of Qualitative Research, 3rd Edition," consists of Part III of the handbook ("Strategies of Inquiry"). "Strategies of Qualitative Inquiry, Third Edition" presents the major tactics--historically, the research methods--that…

  8. Qualitätsmerkmale von RFID Etiketten

    OpenAIRE

    Bernhard, Josef; Dräger, Tobias

    2014-01-01

    Um die Qualität eines RFID Etiketts objektiv und reproduzierbar bewerten zu können sind entsprechende Qualitätsmerkmale und Prüfverfahren notwendig. In diesem Beitrag wird kurz auf Qualitätsmerkmale im Bezug auf RFID Etiketten eingegangen und die aktuelle Situation bei der Definition von Testkriterien und- verfahren dargestellt.

  9. Publishing Qualitative Research in Counseling Journals

    Science.gov (United States)

    Hunt, Brandon

    2011-01-01

    This article focuses on the essential elements to be included when developing a qualitative study and preparing the findings for publication. Using the sections typically found in a qualitative article, the author describes content relevant to each section, with additional suggestions for publishing qualitative research.

  10. Infusing Qualitative Traditions in Counseling Research Designs

    Science.gov (United States)

    Hays, Danica G.; Wood, Chris

    2011-01-01

    Research traditions serve as a blueprint or guide for a variety of design decisions throughout qualitative inquiry. This article presents 6 qualitative research traditions: grounded theory, phenomenology, consensual qualitative research, ethnography, narratology, and participatory action research. For each tradition, the authors describe its…

  11. Teaching Qualitative Research to Practitioner-Researchers

    Science.gov (United States)

    Cox, Rebecca D.

    2012-01-01

    Practitioner-researchers are well-positioned to apply qualitative methods to the study of significant problems of educational practice. However, while learning the skills of qualitative inquiry, practitioners may be compelled by forces outside of qualitative research classrooms to think quantitatively. In this article, the author considers two…

  12. Calorimetric assay of minor actinides

    Energy Technology Data Exchange (ETDEWEB)

    Rudy, C.; Bracken, D.; Cremers, T.; Foster, L.A.; Ensslin, N.

    1996-12-31

    This paper reviews the principles of calorimetric assay and evaluates its potential application to the minor actinides (U-232-4, Am-241, Am- 243, Cm-245, Np-237). We conclude that calorimetry and high- resolution gamma-ray isotopic analysis can be used for the assay of minor actinides by adapting existing methodologies for Pu/Am-241 mixtures. In some cases, mixtures of special nuclear materials and minor actinides may require the development of new methodologies that involve a combination of destructive and nondestructive assay techniques.

  13. Calorimetric assay of minor actinides

    International Nuclear Information System (INIS)

    This paper reviews the principles of calorimetric assay and evaluates its potential application to the minor actinides (U-232-4, Am-241, Am- 243, Cm-245, Np-237). We conclude that calorimetry and high- resolution gamma-ray isotopic analysis can be used for the assay of minor actinides by adapting existing methodologies for Pu/Am-241 mixtures. In some cases, mixtures of special nuclear materials and minor actinides may require the development of new methodologies that involve a combination of destructive and nondestructive assay techniques

  14. QUALITATIVE METHODS IN CREATIVITY STUDIES

    DEFF Research Database (Denmark)

    Hertel, Frederik

    2015-01-01

    -specific methods, involving a discussion of creativity test, divergent and convergent thinking, for studying creativity in this specific setting. Beside from that we will develop a research design involving a combination of methods necessary for conducting a case study in the setting mentioned.......In this article we will focus on developing a qualitative research design suitable for conducting case study in creativity. The case is a team of workers (See Hertel, 2015) doing industrial cleaning in the Danish food industry. The hypothesis is that these workers are both participating in, and...

  15. A comparison of quantitative and qualitative superoxide dismutase assays for application to low temperature microalgae

    NARCIS (Netherlands)

    Janknegt, P.J.; Rijstenbil, J.W.; van de Poll, W.H.; Gechev, T.S.; Buma, A.G.J.

    2007-01-01

    Antioxidant enzymes such as superoxide dismutase (SOD) play a key role in the removal of reactive oxygen species produced during visible and ultraviolet irradiance stress in microalgae and plants. However, little is known about the enzymatic antioxidative stress responses in ecologically important A

  16. What are Millian Qualitative Superiorities?

    Directory of Open Access Journals (Sweden)

    Jonathan Riley

    2008-04-01

    Full Text Available In an article published in Prolegomena 2006, Christoph Schmidt-Petri has defended his interpretation and attacked mine of Mill’s idea that higher kinds of pleasure are superior in quality to lower kinds, regardless of quantity. Millian qualitative superiorities as I understand them are infinite superiorities. In this paper, I clarify my interpretation and show how Schmidt-Petri has misrepresented it and ignored the obvious textual support for it. As a result, he fails to understand how genuine Millian qualitative superiorities determine the novel structure of Mill’s pluralistic utilitarianism, in which a social code of justice that distributes equal rights and duties takes absolute priority over competing considerations. Schmidt-Petri’s own interpretation is a non-starter, because it does noteven recognize that Mill is talking about different kinds of pleasant feelings, such that the higher kinds are intrinsically more valuable than the lower. I conclude by outlining why my interpretation is free of any metaphysical commitment to the “essence” of pleasure.

  17. Qualitative interpretation of galaxy spectra

    CERN Document Server

    Almeida, J Sanchez; Terlevich, E; Fernandes, R Cid; Morales-Luis, A B

    2012-01-01

    We describe a simple step-by-step guide to qualitative interpretation of galaxy spectra. Rather than an alternative to existing automated tools, it is put forward as an instrument for quick-look analysis, and for gaining physical insight when interpreting the outputs provided by automated tools. Though the recipe is of general application, it was developed for understanding the nature of the Automatic Spectroscopic K-means based (ASK) template spectra. They resulted from the classification of all the galaxy spectra in the Sloan Digital Sky Survey data release 7 (SDSS-DR7), thus being a comprehensive representation of the galaxy spectra in the local universe. Using the recipe, we give a description of the properties of the gas and the stars that characterize the ASK classes, from those corresponding to passively evolving galaxies, to HII galaxies undergoing a galaxy-wide starburst. The qualitative analysis is found to be in excellent agreement with quantitative analyses of the same spectra. A number of byprodu...

  18. Correlation between the genotoxicity endpoints measured by two different genotoxicity assays: comet assay and CBMN assay

    Directory of Open Access Journals (Sweden)

    Carina Ladeira

    2015-06-01

    The results concerning of positive findings by micronuclei and non significant ones by comet assay, are corroborated by Deng et al. (2005 study performed in workers occupationally exposed to methotrexate, also a cytostatic drug. According to Cavallo et al. (2009, the comet assay seems to be more suitable for the prompt evaluation of the genotoxic effects, for instance, of polycyclic aromatic hydrocarbons mixtures containing volatile substances, whereas the micronucleus test seems more appropriate to evaluate the effects of exposure to antineoplastic agents. However, there are studies that observed an increase in both the comet assay and the micronucleus test in nurses handling antineoplastic drugs, although statistical significance was only seen in the comet assay, quite the opposite of our results (Maluf & Erdtmann, 2000; Laffon et al. 2005.

  19. The Physcomitrella patens System for Transient Gene Expression Assays.

    Science.gov (United States)

    Thévenin, Johanne; Xu, Wenjia; Vaisman, Louise; Lepiniec, Loïc; Dubreucq, Bertrand; Dubos, Christian

    2016-01-01

    Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells. PMID:27557766

  20. Pluralism in qualitative research: the impact of different researchers and qualitative approaches on the analysis of qualitative data.

    OpenAIRE

    Frost, Nollaig; Nolas, Sevasti-Melissa; Brooks-Gordon, Belinda; Esin, Cigdem; Holt, Amanda; Mehdizadeh, Leila; Shinebourne, Pnina

    2010-01-01

    Qualitative approaches to research in psychology and the social sciences are increasingly used. The variety of approaches incorporates different epistemologies, theoretical traditions and practices with associated analysis techniques spanning a range of theoretical and empirical frameworks. Despite the increase in mixed method approaches it is unusual for qualitative methods to be used in combination with each other. The Pluralism in Qualitative Research project (PQR) was developed in order ...

  1. Contradictions in qualitative management research

    DEFF Research Database (Denmark)

    Hansen, Per Richard; Dorland, Jens

    2016-01-01

    Purpose: Contradictory accounts in empirical material are often perceived as deliberate “lies” or “misleading deceptions” performed in acts of impression management, or they are simply neglected. When observed in the material collected empirically, methods have been developed in order to identify...... and remove them from the analytical work. The purpose of this paper is to re-visit and re-introduce a dissensus-based management research strategy in order to analytically be able to work with what appear to be contradictions and misinformation in qualitative research accounts, and give them a more...... profound role in the understanding of management ideas, work and practices. Design/methodology/approach: A literature review is presented on consensus and dissensus orientated theories on contradictions and multiple and conflicting identities in a single individual in an ethnographic inquiry. The purpose...

  2. QUALITATIVE INTERPRETATION OF GALAXY SPECTRA

    International Nuclear Information System (INIS)

    We describe a simple step-by-step guide to qualitative interpretation of galaxy spectra. Rather than an alternative to existing automated tools, it is put forward as an instrument for quick-look analysis and for gaining physical insight when interpreting the outputs provided by automated tools. Though the recipe is for general application, it was developed for understanding the nature of the Automatic Spectroscopic K-means-based (ASK) template spectra. They resulted from the classification of all the galaxy spectra in the Sloan Digital Sky Survey data release 7, thus being a comprehensive representation of the galaxy spectra in the local universe. Using the recipe, we give a description of the properties of the gas and the stars that characterize the ASK classes, from those corresponding to passively evolving galaxies, to H II galaxies undergoing a galaxy-wide starburst. The qualitative analysis is found to be in excellent agreement with quantitative analyses of the same spectra. We compare the mean ages of the stellar populations with those inferred using the code STARLIGHT. We also examine the estimated gas-phase metallicity with the metallicities obtained using electron-temperature-based methods. A number of byproducts follow from the analysis. There is a tight correlation between the age of the stellar population and the metallicity of the gas, which is stronger than the correlations between galaxy mass and stellar age, and galaxy mass and gas metallicity. The galaxy spectra are known to follow a one-dimensional sequence, and we identify the luminosity-weighted mean stellar age as the affine parameter that describes the sequence. All ASK classes happen to have a significant fraction of old stars, although spectrum-wise they are outshined by the youngest populations. Old stars are metal-rich or metal-poor depending on whether they reside in passive galaxies or in star-forming galaxies.

  3. QUALITATIVE INTERPRETATION OF GALAXY SPECTRA

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez Almeida, J.; Morales-Luis, A. B. [Instituto de Astrofisica de Canarias, E-38205 La Laguna, Tenerife (Spain); Terlevich, R.; Terlevich, E. [Instituto Nacional de Astrofisica, Optica y Electronica, Tonantzintla, Puebla (Mexico); Cid Fernandes, R., E-mail: jos@iac.es, E-mail: abml@iac.es, E-mail: rjt@ast.cam.ac.uk, E-mail: eterlevi@inaoep.mx, E-mail: cid@astro.ufsc.br [Departamento de Fisica-CFM, Universidade Federal de Santa Catarina, P.O. Box 476, 88040-900 Florianopolis, SC (Brazil)

    2012-09-10

    We describe a simple step-by-step guide to qualitative interpretation of galaxy spectra. Rather than an alternative to existing automated tools, it is put forward as an instrument for quick-look analysis and for gaining physical insight when interpreting the outputs provided by automated tools. Though the recipe is for general application, it was developed for understanding the nature of the Automatic Spectroscopic K-means-based (ASK) template spectra. They resulted from the classification of all the galaxy spectra in the Sloan Digital Sky Survey data release 7, thus being a comprehensive representation of the galaxy spectra in the local universe. Using the recipe, we give a description of the properties of the gas and the stars that characterize the ASK classes, from those corresponding to passively evolving galaxies, to H II galaxies undergoing a galaxy-wide starburst. The qualitative analysis is found to be in excellent agreement with quantitative analyses of the same spectra. We compare the mean ages of the stellar populations with those inferred using the code STARLIGHT. We also examine the estimated gas-phase metallicity with the metallicities obtained using electron-temperature-based methods. A number of byproducts follow from the analysis. There is a tight correlation between the age of the stellar population and the metallicity of the gas, which is stronger than the correlations between galaxy mass and stellar age, and galaxy mass and gas metallicity. The galaxy spectra are known to follow a one-dimensional sequence, and we identify the luminosity-weighted mean stellar age as the affine parameter that describes the sequence. All ASK classes happen to have a significant fraction of old stars, although spectrum-wise they are outshined by the youngest populations. Old stars are metal-rich or metal-poor depending on whether they reside in passive galaxies or in star-forming galaxies.

  4. Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes

    OpenAIRE

    Barbau-Piednoir, Elodie; Botteldoorn, Nadine; Yde, Marc; Mahillon, Jacques; Roosens, Nancy H

    2012-01-01

    A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) programme. The paper also proposes a new validation procedure to specifically validate qPCR assays applied to food microbiology according to two guidelines: the ISO 22118 norm and the “Definition of minim...

  5. HTA and synthesis of qualitative research

    DEFF Research Database (Denmark)

    Draborg, Eva Ulriksen; Hansen, Helle Ploug

    2007-01-01

    these kinds of questions. Key challenges using qualitative research in HTA are related to the interpretive and small scale nature of qualitative research and how to synthesise qualitative research. Objective: The objective of this study is to examine and discuss the relevance of synthesis of qualitative...... there are only superficial discussions of the concept of evidence, the relationship between qualitative and quantitative research and the use of theory in the synthesising process. Conclusions: SQR is still a new methodology. The study shows the importance of developing SQR methodology to face the needs...... research (SQR) in HTAs and to address its possibilities and limitations. Methods: SQR is described and discussed focusing on definition of synthesis and of qualitative versus quantitative methods, on questions of evidence and on the relevance for HTA. SQR is understood as an umbrella term for different...

  6. Qualitative perspectives: through a methodological lens

    OpenAIRE

    Lee Parker

    2014-01-01

    Purpose –This paper aims to offer an insight into the emergent qualitative methodological profile and its distinctive contribution to accounting and management scholarship, particularly reflecting upon the contribution of Qualitative Research in Accounting & Management (QRAM). Design/methodology/approach - It examines the range of qualitative methodologies employed in the research published across the ten years of QRAM and analyses the methodological discourse and its contribution to the armo...

  7. USING QUALITATIVE APPROACH IN SPECIAL EDUCATION RESEARCH

    Directory of Open Access Journals (Sweden)

    Natasa GALEVSKA

    1998-04-01

    Full Text Available The text deals with some methodological problems in special education research. The limits of purely positivistic, quantitative, experimental research in the area of special education lately are overcome with the use of qualitative approach. Qualitative research are flexibly designed. The data are descriptive and collected in natural setting. Characteristics of the qualitative research make them more appropriate for investigation of the phenomena in special education, considering the small numbers of available subjects, heterogeneity, ethical and moral problems, etc.

  8. Qualitative research: resurgence, institutionalisation and application

    OpenAIRE

    Fielding, NG

    2005-01-01

    The way that the social sciences developed in respect of methodological preferences, and differences between European and North American approaches, helps us to understand why secondary analysis has until recently been a limited practice in qualitative research. To unravel the developments that explain the differing circumstances of secondary analysis in quantitative and qualitative research, we will initially consider the early days of qualitative method, and comment on its location in the f...

  9. Personal construct psychology methods for qualitative research

    OpenAIRE

    Burr, Vivien; King, Nigel; Butt, Trevor

    2014-01-01

    Personal construct psychology (PCP) has always been better known for its methods than its theory, but many researchers are not aware of the range of qualitative methods offered by a PCP approach. We argue that PCP methods have been overlooked as tools for the qualitative researcher and that they satisfy some key requirements of much qualitative research, such as the capacity to provide in-depth insight into personal experience, to establish a ‘democratic’ relationship between researcher and p...

  10. Validity, reliability, and generalizability in qualitative research

    OpenAIRE

    Leung, Lawrence

    2015-01-01

    In general practice, qualitative research contributes as significantly as quantitative research, in particular regarding psycho-social aspects of patient-care, health services provision, policy setting, and health administrations. In contrast to quantitative research, qualitative research as a whole has been constantly critiqued, if not disparaged, by the lack of consensus for assessing its quality and robustness. This article illustrates with five published studies how qualitative research c...

  11. The Futures of Qualitative Social Research

    OpenAIRE

    Keller, Reiner

    2014-01-01

    In this contribution I begin by reviewing past views on the future of qualitative social research. In different ways, all of these views give the same account of a problematic present state which must be overcome by following their own particular "mandatory directives" for future developments. I then discuss four structural mechanisms from which current problems in the transmission of qualitative and interpretative designs or approaches originate. Recently, supporters of "post-qualitative res...

  12. Conducting qualitative research in audiology : A tutorial

    OpenAIRE

    Knudsen, Line V; Laplante-Levesque, Ariane; Jones, Lesley; Preminger, Jill E.; Nielsen, Claus; Lunner, Thomas; Hickson, Louise; Naylor, Graham; Kramer, Sophia E.

    2012-01-01

    OBJECTIVE: Qualitative research methodologies are being used more frequently in audiology as it allows for a better understanding of the perspectives of people with hearing impairment. This article describes why and how international interdisciplinary qualitative research can be conducted. DESIGN: This paper is based on a literature review and our recent experience with the conduction of an international interdisciplinary qualitative study in audiology. RESULTS: We describe some available qua...

  13. The problem of appraising qualitative research

    OpenAIRE

    Dixon-Woods, M.; Shaw, R; Agarwal, S.; Smith, J.

    2004-01-01

    

 Qualitative research can make a valuable contribution to the study of quality and safety in health care. Sound ways of appraising qualitative research are needed, but currently there are many different proposals with few signs of an emerging consensus. One problem has been the tendency to treat qualitative research as a unified field. We distinguish universal features of quality from those specific to methodology and offer a set of minimally prescriptive prompts to assist with the assessme...

  14. Qualitative Methods in Mental Health Services Research

    OpenAIRE

    Palinkas, Lawrence A.

    2014-01-01

    Qualitative and mixed methods play a prominent role in mental health services research. However, the standards for their use are not always evident, especially for those not trained in such methods. This paper reviews the rationale and common approaches to using qualitative and mixed methods in mental health services and implementation research based on a review of the papers included in this special series along with representative examples from the literature. Qualitative methods are used t...

  15. Qualitative methods in environmental health research.

    OpenAIRE

    Brown, Phil

    2003-01-01

    Public health researchers increasingly turn to qualitative methods either on their own or in combination with quantitative methods. Qualitative methods are especially important to community environmental health research, as they provide a way to produce community narratives that give voice to individuals and characterize the community in a full and complex fashion. This article first traces the legacy of qualitative research in environmental health, then uses a case study of the author's expe...

  16. THE DURATION AND PHASES OF QUALITATIVE RESEARCH

    OpenAIRE

    Natasha ANGELOSKA-GALEVSKA

    1998-01-01

    Text points to the main characteristics of the research process when we use qualitative approach. Qualitative research design differs from the conventional linear model, which is typical for quantitative research. Qualitative research begins with selection of a research problem and then follows a cyclical pattern in which changes can be made in any phase of the research. New questions, tasks and hypotheses generate during the research and collection and analysis of data can be done in success...

  17. Rocket engine diagnostics using qualitative modeling techniques

    Science.gov (United States)

    Binder, Michael; Maul, William; Meyer, Claudia; Sovie, Amy

    1992-01-01

    Researchers at NASA Lewis Research Center are presently developing qualitative modeling techniques for automated rocket engine diagnostics. A qualitative model of a turbopump interpropellant seal system has been created. The qualitative model describes the effects of seal failures on the system steady-state behavior. This model is able to diagnose the failure of particular seals in the system based on anomalous temperature and pressure values. The anomalous values input to the qualitative model are generated using numerical simulations. Diagnostic test cases include both single and multiple seal failures.

  18. New Directions in Qualitative Research in Psychology

    DEFF Research Database (Denmark)

    Demuth, Carolin

    2015-01-01

    Qualitative Research gains increasing popularity in the field of Psychology. With the renewed interest, there are, however, also some risks related to the overhomogenization and increasing standardization of qualitative methods. This special issue is dedicated to clarify some of the existing...... misconceptions of qualitative research and to discuss its potentials for the field of psychology in light of recent endeavors to overcome paradigmatic battles and a re-orientation to the specifities of psychology. The issue comprises a discussion from workshop on the future of qualitative research in psychology...

  19. Chromosome aberration assays in Allium

    Energy Technology Data Exchange (ETDEWEB)

    Grant, W.F.

    1982-01-01

    The common onion (Allium cepa) is an excellent plant for the assay of chromosome aberrations after chemical treatment. Other species of Allium (A. cepa var. proliferum, A. carinatum, A. fistulosum and A. sativum) have also been used but to a much lesser extent. Protocols have been given for using root tips from either bulbs or seeds of Allium cepa to study the cytological end-points, such as chromosome breaks and exchanges, which follow the testing of chemicals in somatic cells. It is considered that both mitotic and meiotic end-points should be used to a greater extent in assaying the cytogenetic effects of a chemical. From a literature survey, 148 chemicals are tabulated that have been assayed in 164 Allium tests for their clastogenic effect. Of the 164 assays which have been carried out, 75 are reported as giving a positive reaction, 49 positive and with a dose response, 1 positive and temperature-related, 9 borderline positive, and 30 negative; 76% of the chemicals gave a definite positive response. It is proposed that the Allium test be included among those tests routinely used for assessing chromosomal damage induced by chemicals.

  20. Bacterial mutagenicity assays: test methods.

    Science.gov (United States)

    Gatehouse, David

    2012-01-01

    The most widely used assays for detecting chemically induced gene mutations are those employing bacteria. The plate incorporation assay using various Salmonella typhimurium LT2 and E. coli WP2 strains is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances capable of causing DNA damage leading to gene mutations. The test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs.The test uses several strains of S. typhimurium which carry different mutations in various genes of the histidine operon, and E. coli which carry the same AT base pair at the critical mutation site within the trpE gene. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. When these auxotrophic bacterial strains are grown on a minimal media agar plates containing a trace of the required amino-acid (histidine or tryptophan), only those bacteria that revert to amino-acid independence (His(+) or Tryp(+)) will grow to form visible colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner.This chapter provides detailed procedures for performing the test in the presence and absence of a metabolic activation system (S9-mix), including advice on specific assay variations and any technical problems. PMID:22147566

  1. Assays for B lymphocyte function.

    Science.gov (United States)

    Bondada, Subbarao; Robertson, Darrell A

    2003-11-01

    This unit describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. The first basic protocol is a generalized system for inducing in vitro antibody production and can accommodate various types of antigens under study. Secreted antibodies can then be measured with an enzyme-linked immunosorbent assay (ELISA) or other soluble-antibody detection systems. Alternatively, the number of antibody-producing cells can be quantified by plaque-forming cell (PFC) assays presented in this unit: the Cunningham-Szenberg and the Jerne-Nordin techniques. Both methods employ specially prepared slide chambers, described here, in which the antibody-producing B cells are mixed with complement and indicator sheep red blood cells (SRBC), or with trinitrophenol-modified SRBC (TNP-SRBC), with subsequent lysis and counting of plaques. Because IgM antibodies fix complement efficiently, whereas IgG and IgA antibodies do not, unmodified PFC assays measure only IgM antibodies. The assay can be modified, however, to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells, as described in alternate protocols. Yet another method of measuring the number of antibody-producing B cells (in a class-specific fashion) is to use the ELISPOT technique described in UNIT 7.14. The resting B cells used in these procedures are prepared as described in the final support protocols for Percoll gradient centrifugation. PMID:18432909

  2. Qualitative Psychology Nexus, Vol. VI: Qualitative psychology in the changing academic context

    OpenAIRE

    Maslo, Irina; Kiegelmann, Mechthild; Huber, Günter; Gento, Samuel; Medina, Antonio; Domínguez, Concepción; Herfort, Inge; Weiss, Andreas; Witzel, Andreas; Ullrich, Annette; Wood, Wendy; Cook, Leslie; Pigozne, Tamara; Sánchez Romero, Cristina; Surikova, Svetlana

    2008-01-01

    The sixth volume of the Qualitative Psychology Nexus comprises documents and contributions of the sixth international meeting on qualitative psychology, organized by EARLI SIG 17 "Qualitative and Quantitative Approaches to Learning and Instruction". The meeting was organised by the Centre for Qualitative Psychology and the University of Latvia to deepen our joint understanding of learning and instruction in the changing academic context. It took place in Riga (Latvia), October 20-22, 2006 as ...

  3. Getting added value from using qualitative research with randomized controlled trials: a qualitative interview study.

    OpenAIRE

    O Cathain, A.; Goode, J.; Drabble, S J; Thomas, K.J.; Rudolph, A; Hewison, J.

    2014-01-01

    Background Qualitative research is undertaken with randomized controlled trials of health interventions. Our aim was to explore the perceptions of researchers with experience of this endeavour to understand the added value of qualitative research to the trial in practice. Methods A telephone semi-structured interview study with 18 researchers with experience of undertaking the trial and/or the qualitative research. Results Interviewees described the added value of qualitative research for the...

  4. Generic qualitative research: a design for qualitative research in emergency care?

    OpenAIRE

    Cooper, S.; Endacott, R

    2007-01-01

    The frequency of qualitative studies in the Emergency Medicine Journal, while still low, has increased over the last few years. All take a generic approach and rarely conform to established qualitative approaches such as phenomenology, ethnography and grounded theory. This generic approach is no doubt selected for pragmatic reasons but can be weakened by a lack of rigor and understanding of qualitative research. This paper explores qualitative approaches and then focuses on “best practice” fo...

  5. Is Qualitative Research Second Class Science? A Quantitative Longitudinal Examination of Qualitative Research in Medical Journals

    OpenAIRE

    Shuval, K.; Harker, K.; Roudsari, B; Groce, N. E.; Mills, B.; Siddiqi, Z.; Shachak, A.

    2011-01-01

    Background: Qualitative research appears to be gaining acceptability in medical journals. Yet, little is actually known about the proportion of qualitative research and factors affecting its publication. This study describes the proportion of qualitative research over a 10 year period and correlates associated with its publication. Design: A quantitative longitudinal examination of the proportion of original qualitative research in 67 journals of general medicine during a 10 year period (1998...

  6. Qualitative methods in pharmacy practice research

    DEFF Research Database (Denmark)

    Kaae, Susanne; Traulsen, Janine Marie

    2015-01-01

    Qualitative research within pharmacy practice is concerned with understanding the behavior of actors such as pharmacy staff, pharmacy owners, patients, other healthcare professionals, and politicians to explore various types of existing practices and beliefs in order to improve them. As qualitative...

  7. Qualitative Research: Emerging Opportunity in Business Education

    Science.gov (United States)

    Gaytan, Jorge

    2007-01-01

    The purpose of this qualitative study was to examine the research methods used in articles published in "The Delta Pi Epsilon Journal" and the "NABTE Review" between 2001 and 2005 to determine the extent to which qualitative research methodologies have been employed by researchers and the extent to which these research methodologies were clearly…

  8. The Qualitative Method of Impact Analysis.

    Science.gov (United States)

    Mohr, Lawrence B.

    1999-01-01

    Discusses qualitative methods of impact analysis and provides an introductory treatment of one such approach. Combines an awareness of an alternative causal epistemology with current knowledge of qualitative methods of data collection and measurement to produce an approach to the analysis of impacts. (SLD)

  9. Musical Cognition at Birth: A Qualitative Study

    Science.gov (United States)

    Hefer, Michal; Weintraub, Zalman; Cohen, Veronika

    2009-01-01

    This paper describes research on newborns' responses to music. Video observation and electroencephalogram (EEG) were collected to see whether newborns' responses to random sounds differed from their responses to music. The data collected were subjected to both qualitative and quantitative analysis. This paper will focus on the qualitative study,…

  10. Teaching Qualitative Research: Lessons from Practice

    Science.gov (United States)

    Poulin, Karen L.

    2007-01-01

    Qualitative research has become increasingly perceived as well suited to the advancement of counseling psychology, yet opportunities for formal training in qualitative inquiry remain inconsistently available within and across graduate programs. For the potential contribution of this approach to counseling psychology to be realized, graduate…

  11. Enhance Your Team-Based Qualitative Research

    OpenAIRE

    Fernald, Douglas H.; Duclos, Christine W.

    2005-01-01

    PURPOSE Qualitative research projects often involve the collaborative efforts of a research team. Challenges inherent in teamwork include changes in membership and differences in analytical style, philosophy, training, experience, and skill. This article discusses teamwork issues and tools and techniques used to improve team-based qualitative research.

  12. The Landscape of Qualitative Research. Third Edition

    Science.gov (United States)

    Denzin, Norman K., Ed.; Lincoln, Yvonna, Ed.

    2007-01-01

    This book, the first volume of the paperback versions of the "The SAGE Handbook of Qualitative Research, Third Edition," takes a look at the field from a broadly theoretical perspective, and is composed of the Handbook's Parts I ("Locating the Field"), II ("Major Paradigms and Perspectives"), and VI ("The Future of Qualitative Research"). "The…

  13. Talking and Thinking about Qualitative Research

    Science.gov (United States)

    Ellis, Carolyn; Bochner, Arthur; Denzin, Norman; Lincoln, Yvonna; Morse, Janice; Pelias, Ronald; Richardson, Laurel

    2008-01-01

    This script comes from an edited transcript of a session titled "Talking and Thinking About Qualitative Research," which was part of the 2006 International Congress of Qualitative Inquiry, held at the University of Illinois at Urbana-Champaign on May 4-6, 2006. This special session featured scholars informally responding to questions about their…

  14. Qualitative Studies: Developing Good Research Questions

    Science.gov (United States)

    Bufkin, Melissa A.

    2006-01-01

    Qualitative research is a type of research process that is widely used to give people a voice while researching a particular subject matter. In using this research process, one must understand how important it is to develop research questions within the qualitative research process. The purpose of this article is to aid researchers in the…

  15. Qualitative Research in Counseling Psychology: Conceptual Foundations

    Science.gov (United States)

    Morrow, Susan L.

    2007-01-01

    Beginning with calls for methodological diversity in counseling psychology, this article addresses the history and current state of qualitative research in counseling psychology. It identifies the historical and disciplinary origins as well as basic assumptions and underpinnings of qualitative research in general, as well as within counseling…

  16. Qualitative Clinical Research with Children and Adolescents

    Science.gov (United States)

    Nelson, Mary Lee; Quintana, Stephen M.

    2005-01-01

    This article provides an overview of how qualitative research methods (QRMs) can augment the literature in child and adolescent clinical psychology by contributing to theory and hypothesis building. We discuss the utility of qualitative methods in examining the nature of clinical processes and obtaining deeper understandings about quantitative…

  17. Getting Specific about Qualitative Research Generalizability

    Science.gov (United States)

    Chenail, Ronald J.

    2010-01-01

    The question of generalizability or the usefulness of qualitative research results beyond the confines of the primary site, sample, and study has been hotly debated by qualitative researchers for decades. When examining this question of generalization the first surprising finding is there appears to be no general consensus about the definition,…

  18. Controlling variation in the comet assay

    OpenAIRE

    Collins, Andrew R; El Yamani, Naouale; Lorenzo, Yolanda; Shaposhnikov, Sergey; Brunborg, Gunnar; Azqueta, Amaya

    2014-01-01

    Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples. Do we have to live with high variability, just because the comet assay is a biological assay rather than analytical chemistry? Numerous attempts have been made to limit variability by standardizing the assay protocol, and the critical steps in the assay have been identified; agarose concentration, duration of ...

  19. Searching for proteins of subspecies with diagnostic potential by comparative qualitative proteomic analysis of mycobacterial tuberculins

    OpenAIRE

    Santema, Wiebren; Overdijk, Marije; Barends, Judith; Krijgsveld, Jeroen; Rutten, Victor; Koets, Ad

    2009-01-01

    Abstract Accurate immunodiagnosis of bovine paratuberculosis is among others hampered by the lack of specific antigens. One of the most frequently used antigen preparations is purified protein derivative (PPD), also known as tuberculin. This crude extract has limitations when used in diagnostic assays due to the presence of cross-reactive antigens. The aim of the current study was to systematically analyze the qualitative protein composition of PPD of the major mycobacterial pathog...

  20. Comet Assay in Cancer Chemoprevention.

    Science.gov (United States)

    Santoro, Raffaela; Ferraiuolo, Maria; Morgano, Gian Paolo; Muti, Paola; Strano, Sabrina

    2016-01-01

    The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks. PMID:26608293

  1. Nondestructive assay of sale materials

    International Nuclear Information System (INIS)

    This paper covers three primary areas: (1) reasons for performing nondestructive assay on SALE materials; (2) techniques used; and (3) discussion of investigators' revised results. The study shows that nondestructive calorimetric assay of plutonium offers a viable alternative to traditional wet chemical techniques. For these samples, the precision ranged from 0.4 to 0.6% with biases less than 0.2%. Thus, for those materials where sampling errors are the predominant source of uncertainty, this technique can provide improved accuracy and precision while saving time and money as well as reducing the amount of liquid wastes to be handled. In addition, high resolution gamma-ray spectroscopy measurements of solids can provide isotopic analysis data in a cost effective and timely manner. The timeliness of the method can be especially useful to the plant operator for production control and quality control measurements

  2. Assay of vitamin B12

    International Nuclear Information System (INIS)

    A radioassay is described for vitamin B12 which involves denaturing serum protein binding proteins with alkali. In the denaturation step a dithiopolyol and cyanide are used and in the intrinsic factor assay step a vitamin B12 analogue such as cobinamide is used to bind with any remaining serum proteins. The invention also includes a kit in which the dithiopolyol is provided in admixture with the alkali. The dithiopolyol may be dithiothreitol or dithioerythritol. (author)

  3. Establishing rigour in qualitative radiography research

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, F.J. [School of Healthcare Professions, University of Salford, Salford M6 6PU (United Kingdom)], E-mail: f.j.murphy@salford.ac.uk; Yielder, J. [Medical Imaging, School of Health Sciences, Unitec, Auckland (New Zealand)

    2010-02-15

    The vast majority of radiography research is subject to critique and evaluation from peers in order to justify the method and the outcome of the study. Within the quantitative domain, which the majority of medical imaging publications tend to fall into, there are prescribed methods for establishing scientific rigour and quality in order to critique a study. However, researchers within the qualitative paradigm, which is a developing area of radiography research, are often unclear about the most appropriate methods to measure the rigour (standards and quality) of a research study. This article considers the issues related to rigour, reliability and validity within qualitative research. The concepts of reliability and validity are briefly discussed within traditional positivism and then the attempts to use these terms as a measure of quality within qualitative research are explored. Alternative methods for research rigour in interpretive research (meanings and emotions) are suggested in order to compliment the existing radiography framework that exists for qualitative studies. The authors propose the use of an established model that is adapted to reflect the iterative process of qualitative research. Although a mechanistic approach to establishing rigour is rejected by many qualitative researchers, it is argued that a guide for novice researchers within a developing research base such as radiography is appropriate in order to establish the credibility and trustworthiness of a qualitative study.

  4. Establishing rigour in qualitative radiography research

    International Nuclear Information System (INIS)

    The vast majority of radiography research is subject to critique and evaluation from peers in order to justify the method and the outcome of the study. Within the quantitative domain, which the majority of medical imaging publications tend to fall into, there are prescribed methods for establishing scientific rigour and quality in order to critique a study. However, researchers within the qualitative paradigm, which is a developing area of radiography research, are often unclear about the most appropriate methods to measure the rigour (standards and quality) of a research study. This article considers the issues related to rigour, reliability and validity within qualitative research. The concepts of reliability and validity are briefly discussed within traditional positivism and then the attempts to use these terms as a measure of quality within qualitative research are explored. Alternative methods for research rigour in interpretive research (meanings and emotions) are suggested in order to compliment the existing radiography framework that exists for qualitative studies. The authors propose the use of an established model that is adapted to reflect the iterative process of qualitative research. Although a mechanistic approach to establishing rigour is rejected by many qualitative researchers, it is argued that a guide for novice researchers within a developing research base such as radiography is appropriate in order to establish the credibility and trustworthiness of a qualitative study.

  5. Qualitative knowledge engineering for nuclear applications

    International Nuclear Information System (INIS)

    After the TMI nuclear power plant accident, the two topics of plant safety and operational efficiency became more important areas of artificial intelligence, which have difference characteristics. Qualitative deep model is the recently prospective technology of AI, that can overcome several handicaps of the existing expert systems such as lack of common sense reasoning. The application of AI to the large and complex system like nuclear power plants is typically and effectively done through a module-based hierarchical system. As each module has to be built with suitable AI system. Through the experiences of hierarchical system construction, we aimed to develop basic AI application schemes for the power plant safety and operational efficiency as well as basic technologies for autonomous power plants. The goal of the research is to develop qualitative reasoning technologies for nuclear power plants. For this purpose, the development of qualitative modeling technologies and qualitative behaviour prediction technologies of the power plant are accomplished. In addition, the feasibility of application of typical qualitative reasoning technologies to power plants is studied . The goal of the application is to develop intelligent control technologies of power plants, support technologies. For these purposes, we analyzed the operation of power plants according to its operation purpose: power generation operation, shut-down and start-up operation. As a result, qualitative model of basic components were sketched, including pipes, valves, pumps and heat exchangers. Finally, plant behaviour prediction technologies through qualitative plant heat transfer model and design support technologies through 2nd-order differential equation were developed. For the construction of AI system of power plants, we have studied on the mixed module based hierarchical software. As a testbed, we have considered the spent fuel system and the feedwater system. We also studied the integration

  6. Correlation between the genotoxicity endpoints measured by two different genotoxicity assays: comet assay and CBMN assay

    OpenAIRE

    Carina Ladeira; Susana Viegas; Gomes, Manuel C.

    2015-01-01

    The cytokinesis-block micronucleus cytome (CBMN) assay is a comprehensive system for measuring DNA damage; cytostasis and cytotoxicity-DNA damage events are scored specifically in once-divided binucleated cells. The endpoints possible to be measured are micronuclei (MN), a biomarker of chromosome breakage and/or whole chromosome loss, nucleoplasmic bridges (NPB), a biomarker of DNA misrepair and/or telomere end-fusions, and nuclear buds (NBUD), a biomarker of elimination of amplified DNA and/...

  7. Qualitative Research on Emergency Medicine Physicians

    DEFF Research Database (Denmark)

    Paltved, Charlotte; Musaeus, Peter

    2012-01-01

    with qualitative research. Methods: Electronic databases of English peer-reviewed articles were searched from 1971 to 2012 using Medline through PubMed and PsychINFO. This search was supplemented with hand-searches of Academic Emergency Medicine and Emergency Medicine Journal from 1999 to 2012 and...... cross references were reviewed. The key words used were emergency medicine, qualitative, ethnography, observation, interview, video, anthropology, simulation, and simulation-based. Results: 820 papers were identified and 46 studies were included in this review. This literature review found that the......Aim: This study aims to systematically review the qualitative research studying Emergency Medicine (EM) physicians in Emergency Departments (ED). Background: Qualitative research aims to study complex social phenomena. EM is a highly complex medical and social environment that can be investigated...

  8. Validity and reliability in qualitative researches

    Directory of Open Access Journals (Sweden)

    Mohammad Abbaszadeh

    2012-06-01

    Full Text Available Abstract   Today science world changes sharply in a high speed. In a way that in all areas such as research methods these changes are obvious. Although concepts such as validity and reliability in Positivist methodology have been used with distinct and general rules, in post positivist period in which they have paid much more attention to qualitative research, these principles have lost their general form. In one hand some thinkers stress on applying common validity and reliability quantitative techniques in qualitative studies, on the other hand, some groups focus on distinction between qualitative and quantitative methods. Therefore, in this study we try to introduce the qualitative research methods and applying validity and reliability concepts in this way.  .

  9. Perils and potentials in qualitative psychology

    DEFF Research Database (Denmark)

    Brinkmann, Svend

    2015-01-01

    Famously, Ebbinghaus declared that psychology has a long past, but only a short history. Psychology, as something implicit to human conduct, is as old as the human race, but the science, as an explicit investigative reflection upon that conduct, is a recent invention. Within the short history of...... psychology, we find an even shorter history of qualitative psychology specifically. Although most founding fathers (Freud, Piaget, Bartlett etc.) worked as “qualitative psychologists”, they found no need to thematize their methods of inquiry in this manner. Since around 1980, however, a field has established...... itself that can be called qualitative psychology. In this paper, I discuss how this field can move sensibly into the future, and I highlight two perils and two potentials. The perils stem from neo-positivism and a threatening “McDonaldization” of qualitative research, while the potentials are related to...

  10. Designs, formats and applications of lateral flow assay: A literature review

    Directory of Open Access Journals (Sweden)

    Muhammad Sajid

    2015-11-01

    Full Text Available This manuscript provides a brief overview of latest research involving the use of lateral flow assay for qualitative and quantitative analysis in different areas. The excellent features and versatility of detection formats make these strips an ideal choice for point of care applications. We outline and critically discuss detection formats, molecular recognition probes, labels, and detection systems used in lateral flow assay. Applications in different fields along with selected examples from the literature have been included to show analytical performance of these devices. At the end, we summarize accomplishments, weaknesses and future challenges in the area of lateral flow strips.

  11. Capture-S, a nontreponemal solid-phase erythrocyte adherence assay for serological detection of syphilis.

    OpenAIRE

    Stone, D L; Moheng, M C; Rolih, S; Sinor, L T

    1997-01-01

    A solid-phase erythrocyte adherence assay has been developed for the serological detection of reagin antibodies in syphilis. Capture-S (Immucor, Inc., Norcross, Ga.) is a nontreponemal, qualitative screening test for the detection of immunoglobulin G (IgG) and IgM antilipid antibodies in serum or plasma samples from blood donors. The Capture-S assay utilizes a modified Venereal Disease Research Laboratory antigen bound to microtitration wells and anti-IgG- plus anti-IgM-coated indicator eryth...

  12. Cross-Cultural Research and Qualitative Inquiry

    OpenAIRE

    Pranee Liamputtong

    2010-01-01

    Cross-cultural research has become important in this postmodern world where many people have been made, and are still, marginalised and vulnerable by others in more powerful positions like colonial researchers. In this paper, I contend that qualitative research is particularly appropriate for cross-cultural research because it allows us to find answers which are more relevant to the research participants. Cross-cultural qualitative research must be situated within some theoretical frameworks....

  13. Researcher's reflexivity and qualitative methods in psychology

    OpenAIRE

    Franz Breuer; Antje Lettau

    2007-01-01

    Within academic psychology in the German-speaking countries qualitative methodology plays only a marginal role. Most researchers favour a quantitative methodological orientation with focus on experimental approaches and mathematical or statistical procedures of data analysis. Opposed to this orientation qualitative methodology often stands in epistemological traditions that pay attention to personal preconditions of the researcher and their influence on the research process. Of interest are s...

  14. Autism and the Family: A Qualitative Perspective

    OpenAIRE

    Glass, Paul William

    2001-01-01

    AUTISM AND THE FAMILY: A QUALITATIVE PERSPECTIVE Paul W. Glass (ABSTRACT) The focus of this dissertation was to gain a better understanding of autism, and its effects on family life. Studies have been done on the behavioral and cognitive effects of autism on the affected child, and how those effects manifest themselves into family life. No studies were found, however, that give a rich, qualitative account of what it is like to live with autism using first hand accounts as data, and ...

  15. Theory and evidence in qualitative research

    OpenAIRE

    Hammersley, Martyn

    1995-01-01

    This article identifies and explores some unresolved problems surrounding the role of theory and evidence in qualitative research. It begins by distinguishing among various meanings of the term theory, focusing in particular on the view that portrays it as consisting of specific explanatory principles. An ambivalence towards theory on the part of qualitative researchers is highlighted, showing how the influence of positivism persists despite explicit rejection of it. The problems involved in ...

  16. Rural poverty in Ecuador : a qualitative assessment

    OpenAIRE

    Hentschel, Jesko; Waters, William F; Vandever Webb, Anna Kathryn

    1996-01-01

    A complement to recent in-depth quantitative analyses of rural poverty in Ecuador, this is a report on the results of the Rural Qualitative Assessment in living conditions in rural communities in all three Ecuador's diverse regions. Using a variety of qualitative techniques, the research aimed to assess what poverty means to marginalized rural families, what kind of survival strategies families use in times of hardship, and what these families believe is needed to alleviate poverty. Several k...

  17. Modal Logics for Qualitative Possibility and Beliefs

    OpenAIRE

    Boutilier, Craig

    2013-01-01

    Possibilistic logic has been proposed as a numerical formalism for reasoning with uncertainty. There has been interest in developing qualitative accounts of possibility, as well as an explanation of the relationship between possibility and modal logics. We present two modal logics that can be used to represent and reason with qualitative statements of possibility and necessity. Within this modal framework, we are able to identify interesting relationships between possibilistic logic, beliefs ...

  18. Qualitative reasoning for earthquake resistant buildings

    OpenAIRE

    Bozzo Rotondo, Luis Miguel

    1993-01-01

    Qualitative reasoning is a new field of research from artificial intelligence that derives the behavior of a model from a high- level representation of the fundamental principles of the domain, and the geometry and topology of the model. Qualitative reasoning is useful for evaluating conceptual designs for earthquake resistant buildings because it derives values for parameters even with incomplete and imprecise knowledge about the model, which is particularly important for the conceptual desi...

  19. Freedom to Speak Up - Qualitative Research Report

    OpenAIRE

    Vandekerckhove, Wim; Rumyantseva, Nataliya

    2015-01-01

    This is the report from a qualitative research study commissioned by The Freedom to Speak Up Review, which set out to be an independent review into creating an open and honest reporting culture in the NHS. This qualitative study aimed to: • gain an understanding of views and attitudes to whistleblowing in the NHS held by those in various roles in the whistleblowing process - i.e. whistleblowers, frontline staff, managers, directors, regulators, unions, and whistleblowing support group...

  20. Analyzing qualitative data with computer software.

    OpenAIRE

    Weitzman, E A

    1999-01-01

    OBJECTIVE: To provide health services researchers with an overview of the qualitative data analysis process and the role of software within it; to provide a principled approach to choosing among software packages to support qualitative data analysis; to alert researchers to the potential benefits and limitations of such software; and to provide an overview of the developments to be expected in the field in the near future. DATA SOURCES, STUDY DESIGN, METHODS: This article does not include rep...

  1. Theoretical versus pragmatic design in qualitative research

    OpenAIRE

    Smith, Joanna; Bekker, Hilary; Cheater, Francine

    2011-01-01

    For many years, discussions of the relative merits of generic and theoretical approaches to qualitative research have divided researchers while overshadowing the need to focus on addressing clinical questions. Drawing on the challenges of designing a study that explored parents’ experiences of living with children with hydrocephalus, the authors of this paper argue that over-adherence to, and deliberations about, the philosophical origins of qualitative methods is undermining the contribution...

  2. Research MethodologyOverview of Qualitative Research

    OpenAIRE

    Grossoehme, Daniel H.

    2014-01-01

    Qualitative research methods are a robust tool for chaplaincy research questions. Similar to much of chaplaincy clinical care, qualitative research generally works with written texts, often transcriptions of individual interviews or focus group conversations and seeks to understand the meaning of experience in a study sample. This article describes three common methodologies: ethnography, grounded theory, and phenomenology. Issues to consider relating to the study sample, design, and analysis...

  3. Radiosotopic assay and binder therefor

    International Nuclear Information System (INIS)

    A rapid and less costly radioisotopic assay for measuring the concentration of folate in blood serum is described. This procedure utilizes 3H-pteroylmonoglutamate, unlabeled 5-methyltetrahydrofolic acid, and a partially purified folate binder, such as for example a folate binder extracted from hog kidney. The procedure involves radioisotopically relating the bound amounts of a labeled folate and a known folate at various concentrations of the known folate in a system containing a predetermined amount of the labeled folate, a predetermined amount of the binder factor for the folates, and a predetermined amount of defolated test serum. 16 claims, 8 drawing figures

  4. Methodological pluralism as a vehicle of qualitative generalization

    DEFF Research Database (Denmark)

    Schrøder, Kim Christian

    2012-01-01

    This article proposes an innovative framework for making analytical generalizations in qualitative research. In order to achieve this purpose it contends that the multimethodological strategy, now readily accepted in many qualitative quarters, of mixing qualitative and quantitative methods...

  5. The chemistry behind antioxidant capacity assays.

    Science.gov (United States)

    Huang, Dejian; Ou, Boxin; Prior, Ronald L

    2005-03-23

    This review summarizes the multifaceted aspects of antioxidants and the basic kinetic models of inhibited autoxidation and analyzes the chemical principles of antioxidant capacity assays. Depending upon the reactions involved, these assays can roughly be classified into two types: assays based on hydrogen atom transfer (HAT) reactions and assays based on electron transfer (ET). The majority of HAT-based assays apply a competitive reaction scheme, in which antioxidant and substrate compete for thermally generated peroxyl radicals through the decomposition of azo compounds. These assays include inhibition of induced low-density lipoprotein autoxidation, oxygen radical absorbance capacity (ORAC), total radical trapping antioxidant parameter (TRAP), and crocin bleaching assays. ET-based assays measure the capacity of an antioxidant in the reduction of an oxidant, which changes color when reduced. The degree of color change is correlated with the sample's antioxidant concentrations. ET-based assays include the total phenols assay by Folin-Ciocalteu reagent (FCR), Trolox equivalence antioxidant capacity (TEAC), ferric ion reducing antioxidant power (FRAP), "total antioxidant potential" assay using a Cu(II) complex as an oxidant, and DPPH. In addition, other assays intended to measure a sample's scavenging capacity of biologically relevant oxidants such as singlet oxygen, superoxide anion, peroxynitrite, and hydroxyl radical are also summarized. On the basis of this analysis, it is suggested that the total phenols assay by FCR be used to quantify an antioxidant's reducing capacity and the ORAC assay to quantify peroxyl radical scavenging capacity. To comprehensively study different aspects of antioxidants, validated and specific assays are needed in addition to these two commonly accepted assays. PMID:15769103

  6. Indirect conductimetric assay of antibacterial activities.

    Science.gov (United States)

    Sawai, J; Doi, R; Maekawa, Y; Yoshikawa, T; Kojima, H

    2002-11-01

    The applicability of indirect conductimetric assays for evaluation of antibacterial activity was examined. The minimal inhibitory concentration (MIC) obtained by the indirect method was consistent with that by the direct conductimetric assay and the turbidity method. The indirect assay allows use of growth media, which cannot be used in the direct conductimetric assay, making it possible to evaluate the antibacterial activity of insoluble or slightly soluble materials with high turbidity, such as antibacterial ceramic powders. PMID:12407467

  7. The role of the researcher in qualitative psychology

    OpenAIRE

    2002-01-01

    Volume two of Qualitative Research Nexus focuses on the roles of qualitative researchers and their relationships within psychological studies. This book is a result of the presentations, discussions, and collaborations of participants at the second workshop "Qualitative Psychology" in October 2001 in Blaubeuren, Germany that was organized by the Center for Qualitative Psychology. The theme of the meeting was "the role of the researcher in qualitative psychology." Reading this volume of Qualit...

  8. Serum indices: managing assay interference.

    Science.gov (United States)

    Farrell, Christopher-John L; Carter, Andrew C

    2016-09-01

    Clinical laboratories frequently encounter samples showing significant haemolysis, icterus or lipaemia. Technical advances, utilizing spectrophotometric measurements on automated chemistry analysers, allow rapid and accurate identification of such samples. However, accurate quantification of haemolysis, icterus and lipaemia interference is of limited value if laboratories do not set rational alert limits, based on sound interference testing experiments. Furthermore, in the context of increasing consolidation of laboratories and the formation of laboratory networks, there is an increasing requirement for harmonization of the handling of haemolysis, icterus and lipaemia-affected samples across different analytical platforms. Harmonization may be best achieved by considering both the analytical aspects of index measurement and the possible variations in the effects of haemolysis, icterus and lipaemia interferences on assays from different manufacturers. Initial verification studies, followed up with ongoing quality control testing, can help a laboratory ensure the accuracy of haemolysis, icterus and lipaemia index results, as well as assist in managing any biases in index results from analysers from different manufacturers. Similarities, and variations, in the effect of haemolysis, icterus and lipaemia interference in assays from different manufacturers can often be predicted from the mechanism of interference. Nevertheless, interference testing is required to confirm expected similarities or to quantify differences. It is important that laboratories are familiar with a number of interference testing protocols and the particular strengths and weaknesses of each. A rigorous approach to all aspects of haemolysis, icterus and lipaemia interference testing allows the analytical progress in index measurement to be translated into improved patient care. PMID:27147624

  9. SNO+ Scintillator Purification and Assay

    International Nuclear Information System (INIS)

    We describe the R and D on the scintillator purification and assay methods and technology for the SNO+ neutrino and double-beta decay experiment. The SNO+ experiment is a replacement of the SNO heavy water with liquid scintillator comprised of 2 g/L PPO in linear alkylbenzene (LAB). During filling the LAB will be transported underground by rail car and purified by multi-stage distillation and steam stripping at a flow rate of 19 LPM. While the detector is operational the scintillator can be recirculated at 150 LPM (full detector volume in 4 days) to provide repurification as necessary by either water extraction (for Ra, K, Bi) or by functional metal scavenger columns (for Pb, Ra, Bi, Ac, Th) followed by steam stripping to remove noble gases and oxygen (Rn, O2, Kr, Ar). The metal scavenger columns also provide a method for scintillator assay for ex-situ measurement of the U and Th chain radioactivity. We have developed ''natural'' radioactive spikes of Pb and Ra in LAB and use these for purification testing. Lastly, we present the planned operating modes and purification strategies and the plant specifications and design.

  10. Proteasome Assay in Cell Lysates

    Science.gov (United States)

    Maher, Pamela

    2016-01-01

    The ubiquitin-proteasome system (UPS) mediates the majority of the proteolysis seen in the cytoplasm and nucleus of mammalian cells. As such it plays an important role in the regulation of a variety of physiological and pathophysiological processes including tumorigenesis, inflammation and cell death (Ciechanover, 2005; Kisselev and Goldberg, 2001). A number of recent studies have shown that proteasome activity is decreased in a variety of neurological disorders including Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis and stroke as well as during normal aging (Chung et al., 2001; Ciechanover and Brundin, 2003; Betarbet et al., 2005). This decrease in proteasome activity is thought to play a critical role in the accumulation of abnormal and oxidized proteins. Protein clearance by the UPS involves two sequential reactions. The first is the tagging of protein lysine residues with ubiquitin (Ub) and the second is the subsequent degradation of the tagged proteins by the proteasome. We herein describe an assay for the second of these two reactions (Valera et al., 2013). This assay uses fluorogenic substrates for each of the three activities of the proteasome: chymotrypsin-like activity, trypsin-like activity and caspase-like activity. Cleavage of the fluorophore from the substrate by the proteasome results in fluorescence that can be detected with a fluorescent plate reader.

  11. SNO+ Scintillator Purification and Assay

    Science.gov (United States)

    Ford, R.; Chen, M.; Chkvorets, O.; Hallman, D.; Vázquez-Jáuregui, E.

    2011-04-01

    We describe the R&D on the scintillator purification and assay methods and technology for the SNO+ neutrino and double-beta decay experiment. The SNO+ experiment is a replacement of the SNO heavy water with liquid scintillator comprised of 2 g/L PPO in linear alkylbenzene (LAB). During filling the LAB will be transported underground by rail car and purified by multi-stage distillation and steam stripping at a flow rate of 19 LPM. While the detector is operational the scintillator can be recirculated at 150 LPM (full detector volume in 4 days) to provide repurification as necessary by either water extraction (for Ra, K, Bi) or by functional metal scavenger columns (for Pb, Ra, Bi, Ac, Th) followed by steam stripping to remove noble gases and oxygen (Rn, O2, Kr, Ar). The metal scavenger columns also provide a method for scintillator assay for ex-situ measurement of the U and Th chain radioactivity. We have developed "natural" radioactive spikes of Pb and Ra in LAB and use these for purification testing. Lastly, we present the planned operating modes and purification strategies and the plant specifications and design.

  12. 21 CFR 864.7525 - Heparin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Heparin assay. 864.7525 Section 864.7525 Food and... HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7525 Heparin assay. (a) Identification. A heparin assay is a device used to determine the level of the anticoagulant heparin in the...

  13. 21 CFR 864.7490 - Sulfhemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sulfhemoglobin assay. 864.7490 Section 864.7490 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... assay. (a) Identification. A sulfhemoglobin assay is a device consisting of the reagents,...

  14. 21 CFR 864.7250 - Erythropoietin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Erythropoietin assay. 864.7250 Section 864.7250... assay. (a) Identification. A erythropoietin assay is a device that measures the concentration of erythropoietin (an enzyme that regulates the production of red blood cells) in serum or urine. This...

  15. 21 CFR 864.7425 - Carboxyhemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Carboxyhemoglobin assay. 864.7425 Section 864.7425 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... assay. (a) Identification. A carboxyhemoglobin assay is a device used to determine the...

  16. 21 CFR 866.3210 - Endotoxin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Endotoxin assay. 866.3210 Section 866.3210 Food... DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3210 Endotoxin assay. (a) Identification. An endotoxin assay is a device that uses serological techniques in whole blood. The device...

  17. Multicentre comparison of a diagnostic assay

    DEFF Research Database (Denmark)

    Waters, Patrick; Reindl, Markus; Saiz, Albert;

    2016-01-01

    ) assays in neuromyelitis optica spectrum disorders (NMOSD). METHODS: Coded samples from patients with neuromyelitis optica (NMO) or NMOSD (101) and controls (92) were tested at 15 European diagnostic centres using 21 assays including live (n=3) or fixed cell-based assays (n=10), flow cytometry (n=4...

  18. Quantification of Analyte Concentration from a Paper-Based Lateral Flow Assay Device Using Reflective Sensors

    OpenAIRE

    Chiu, Megan Z; Linnes, Jacqueline

    2015-01-01

    Paper-based point-of-care (POC) diagnostics is a growing field in global health due to the extreme portability, accuracy, affordability, and ease of use of these tests. Advancements in recent years have led to more accurate detection and improved functionality using multistep molecular diagnostics. Many such assays utilize lateral flow detection strips for visualization of diagnostic results by eye, which limits the results to qualitative Yes/No readouts. This project focused on combining rec...

  19. Establishment of Immunoradiometric Assay for Carcinoembryonic Antigen

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Two anti-CEA monoclonal antibodies are used, one is coated on the microtiter plate, the other is labeled to make 125I-CEAMcAb. The one-step assay is established based on immunoradiometric assay(IRMA). The sensitivity of the assay is 0.5 μ g/L. The intra-assay CVs and the inter-assay CVs are lower than 10.0% and 15.0%, respectively. The analytical recoveries are ranged from 97.4% to 107.8%. The reference cut-out value of 35 normal serum is lower than

  20. Making transuranic assay measurements using modern controllers

    International Nuclear Information System (INIS)

    This paper describes methodology and computer-controlled instrumentation developed at the Los Alamos National Laboratory that accurately performs nondestructive assays of large containers bearing transuranic wastes and nonradioactive matrix materials. These assay systems can measure fissile isotopes with 1-mg sensitivity and spontaneous neutron-emitting isotopes at a 10-mg sensitivity. The assays are performed by neutron interrogation, detection, and counting in a custom assay chamber. An International Business Machines Personal Computer (IBM-PC) is used to control the CAMAC-based instrumentation system that acquires the assay data. 6 refs., 7 figs

  1. The C. elegans healthspan and stress-resistance assay toolkit.

    Science.gov (United States)

    Keith, Scott Alexander; Amrit, Francis Raj Gandhi; Ratnappan, Ramesh; Ghazi, Arjumand

    2014-08-01

    A wealth of knowledge on the genetic mechanisms that govern aging has emerged from the study of mutants that exhibit enhanced longevity and exceptional resilience to adverse environmental conditions. In these studies, lifespan has been an excellent proxy for establishing the rate of aging, but it is not always correlated with qualitative measures of healthy aging or 'healthspan'. Although the attributes of healthspan have been challenging to define, they share some universal features that are increasingly being incorporated into aging studies. Here we describe methods used to determine Caenorhabditis elegans healthspan. These include assessments of tissue integrity and functionality and resistance to a variety of biotic and abiotic stressors. We have chosen to include simple, rapid assays in this collection that can be easily undertaken in any C. elegans laboratory, and can be relied on to provide a preliminary but thorough insight into the healthspan of a population. PMID:24727065

  2. Verification of uranium 238 quantity calculated using waste assay systems

    International Nuclear Information System (INIS)

    The amount of 238U in uranium-contaminated waste drums generated in the decommissioning of nuclear facilities is evaluated from γ-ray measurement. We used the γ-ray measurement system made from CANBERRA(Qualitative and Quantitative (Q2) Low Level Waste Assay Systems) and measured the waste drums. This system assumes uniform distribution of uranium. But, homogeneity can not be checked with real waste drums. Authors developed the new analysis technique which calculates the amount of uranium by correcting the influence of uneven distribution of the uranium. As a result of evaluating using the new analysis technique, the error which influences quantitative value of 238U has been evaluated. (author)

  3. Activity assay of membrane transport proteins

    Institute of Scientific and Technical Information of China (English)

    Hao Xie

    2008-01-01

    Membrane transport proteins are integral membrane proteins and considered as potential drug targets. Activity assay of transport proteins is essential for developing drugs to target these proteins. Major issues related to activity assessment of transport proteins include availability of transporters,transport activity of transporters, and interactions between ligands and transporters. Researchers need to consider the physiological status of proteins (bound in lipid membranes or purified), availability and specificity of substrates, and the purpose of the activity assay (screening, identifying, or comparing substrates and inhibitors) before choosing appropriate assay strategies and techniques. Transport proteins bound in vesicular membranes can be assayed for transporting substrate across membranes by means of uptake assay or entrance counterflow assay. Alternatively, transport proteins can be assayed for interactions with ligands by using techniques such as isothermal titration calorimetry, nuclear magnetic resonance spectroscopy, or surface plasmon resonance. Other methods and techniques such as fluorometry, scintillation proximity assay, electrophysiological assay, or stopped-flow assay could also be used for activity assay of transport proteins. In this paper the major strategies and techniques for activity assessment of membrane transport proteins are reviewed.

  4. Maximum entropy estimation of a Benzene contaminated plume using ecotoxicological assays

    International Nuclear Information System (INIS)

    Ecotoxicological bioassays, e.g. based on Danio rerio teratogenicity (DarT) or the acute luminescence inhibition with Vibrio fischeri, could potentially lead to significant benefits for detecting on site contaminations on qualitative or semi-quantitative bases. The aim was to use the observed effects of two ecotoxicological assays for estimating the extent of a Benzene groundwater contamination plume. We used a Maximum Entropy (MaxEnt) method to rebuild a bivariate probability table that links the observed toxicity from the bioassays with Benzene concentrations. Compared with direct mapping of the contamination plume as obtained from groundwater samples, the MaxEnt concentration map exhibits on average slightly higher concentrations though the global pattern is close to it. This suggest MaxEnt is a valuable method to build a relationship between quantitative data, e.g. contaminant concentrations, and more qualitative or indirect measurements, in a spatial mapping framework, which is especially useful when clear quantitative relation is not at hand. - Highlights: ► Ecotoxicological shows significant benefits for detecting on site contaminations. ► MaxEnt to rebuild qualitative link on concentration and ecotoxicological assays. ► MaxEnt shows similar pattern when compared with concentrations map of groundwater. ► MaxEnt is a valuable method especially when quantitative relation is not at hand. - A Maximum Entropy method to rebuild qualitative relationships between Benzene groundwater concentrations and their ecotoxicological effect.

  5. Non destructive assay (NDA) techniques

    International Nuclear Information System (INIS)

    In the IAEA Safeguards System the basic verification method used is nuclear material accountancy, with containment and surveillance as important complementary measures. If nuclear material accountancy is to be effective, IAEA inspectors have to make independent measurements to verify declared material quantities. Most of the equipment available to the inspectors is designed to measure gamma rays and/or neutrons emitted by various nuclear materials. Equipment is also available to measure the gross weight of an item containing nuclear material. These types of measurement techniques are generally grouped under the title of nondestructive assay (NDA). The paper describes the NDA techniques and instruments used to verify the total amount of nuclear material held at a nuclear facility. (author)

  6. Predictive Assay For Cancer Targets

    Energy Technology Data Exchange (ETDEWEB)

    Suess, A; Nguyen, C; Sorensen, K; Montgomery, J; Souza, B; Kulp, K; Dugan, L; Christian, A

    2005-09-19

    Early detection of cancer is a key element in successful treatment of the disease. Understanding the particular type of cancer involved, its origins and probable course, is also important. PhIP (2-amino-1-methyl-6 phenylimidazo [4,5-b]pyridine), a heterocyclic amine produced during the cooking of meat at elevated temperatures, has been shown to induce mammary cancer in female, Sprague-Dawley rats. Tumors induced by PhIP have been shown to contain discreet cytogenetic signature patterns of gains and losses using comparative genomic hybridization (CGH). To determine if a protein signature exists for these tumors, we are analyzing expression levels of the protein products of the above-mentioned tumors in combination with a new bulk protein subtractive assay. This assay produces a panel of antibodies against proteins that are either on or off in the tumor. Hybridization of the antibody panel onto a 2-D gel of tumor or control protein will allow for identification of a distinct protein signature in the tumor. Analysis of several gene databases has identified a number of rat homologs of human cancer genes located in these regions of gain and loss. These genes include the oncogenes c-MYK, ERBB2/NEU, THRA and tumor suppressor genes EGR1 and HDAC3. The listed genes have been shown to be estrogen-responsive, suggesting a possible link between delivery of bio-activated PhIP to the cell nucleus via estrogen receptors and gene-specific PhIP-induced DNA damage, leading to cell transformation. All three tumors showed similar silver staining patterns compared to each other, while they all were different than the control tissue. Subsequent screening of these genes against those from tumors know to be caused by other agents may produce a protein signature unique to PhIP, which can be used as a diagnostic to augment optical and radiation-based detection schemes.

  7. Comet assay as a predictive assay for radiosensitivity of two human brain tumor cell lines

    International Nuclear Information System (INIS)

    Micronucleus assay and comet assay were compared as a predictive assay for radiosensitivity of tumors. Two human brain tumor cell lines, Becker (derived from astrocytoma) and ONS76 (derived from medulloblastoma) were used. Colony methods as the gold standard showed ONS76 as radiosensitive and Becker as radioresistant cell lines. Micronucleus assay revealed no different radiosensitivity between them. With comet assay, Becker cells received irradiation showed less damage to the DNA and faster repair of the damage than ONS76 cells did. The results correlate with those from colony methods. Comet assay is simple and rapid method for clinical use and it has an advantage not to establish the primary culture. Moreover, the results of comet assay showed not only DNA damage but also repair from the damage. It is concluded that comet assay is a superior method than micronucleus assay and has a potent candidate for clinical predictive assay. (author)

  8. Conducting Qualitative Data Analysis: Reading Line-by-Line, but Analyzing by Meaningful Qualitative Units

    Science.gov (United States)

    Chenail, Ronald J.

    2012-01-01

    In the first of a series of "how-to" essays on conducting qualitative data analysis, Ron Chenail points out the challenges of determining units to analyze qualitatively when dealing with text. He acknowledges that although we may read a document word-by-word or line-by-line, we need to adjust our focus when processing the text for purposes of…

  9. Conducting Qualitative Data Analysis: Qualitative Data Analysis as a Metaphoric Process

    Science.gov (United States)

    Chenail, Ronald J.

    2012-01-01

    In the second of a series of "how-to" essays on conducting qualitative data analysis, Ron Chenail argues the process can best be understood as a metaphoric process. From this orientation he suggests researchers follow Kenneth Burke's notion of metaphor and see qualitative data analysis as the analyst systematically considering the "this-ness" of…

  10. Turning Points in Qualitative Research: Tying Knots in a Handkerchief. Crossroads in Qualitative Inquiry Series.

    Science.gov (United States)

    Lincoln, Yvonna S., Ed.; Denzin, Norman K., Ed.

    The chapters of this volume traces the changes in the discipline of qualitative inquiry over the last five decades. The collection serves as a textbook for training scholars in the history and trajectory of qualitative research. The chapters of part 1, The Revolution of Representation: Feminist and Race/Ethnic Studies Discourses, are: (1) Situated…

  11. Disrupting Qualitative Inquiry: Possibilities and Tensions in Educational Research. Critical Qualitative Research. Volume 10

    Science.gov (United States)

    Brown, Ruth Nicole, Ed.; Carducci, Rozana, Ed.; Kuby, Candace R., Ed.

    2014-01-01

    "Disrupting Qualitative Inquiry" is an edited volume that examines the possibilities and tensions encountered by scholars who adopt disruptive qualitative approaches to the study of educational contexts, issues, and phenomena. It presents a collection of innovative and intellectually stimulating chapters which illustrate the potential…

  12. Building Interdisciplinary Qualitative Research Networks: Reflections on Qualitative Research Group (QRG) at the University of Manitoba

    Science.gov (United States)

    Roger, Kerstin Stieber; Halas, Gayle

    2012-01-01

    As qualitative research methodologies continue to evolve and develop, both students and experienced researchers are showing greater interest in learning about and developing new approaches. To meet this need, faculty at the University of Manitoba created the Qualitative Research Group (QRG), a community of practice that utilizes experiential…

  13. About Statistical Analysis of Qualitative Survey Data

    Directory of Open Access Journals (Sweden)

    Stefan Loehnert

    2010-01-01

    Full Text Available Gathered data is frequently not in a numerical form allowing immediate appliance of the quantitative mathematical-statistical methods. In this paper are some basic aspects examining how quantitative-based statistical methodology can be utilized in the analysis of qualitative data sets. The transformation of qualitative data into numeric values is considered as the entrance point to quantitative analysis. Concurrently related publications and impacts of scale transformations are discussed. Subsequently, it is shown how correlation coefficients are usable in conjunction with data aggregation constrains to construct relationship modelling matrices. For illustration, a case study is referenced at which ordinal type ordered qualitative survey answers are allocated to process defining procedures as aggregation levels. Finally options about measuring the adherence of the gathered empirical data to such kind of derived aggregation models are introduced and a statistically based reliability check approach to evaluate the reliability of the chosen model specification is outlined.

  14. Leveraging Qualitative Reasoning to Learning Manipulation Tasks

    Directory of Open Access Journals (Sweden)

    Diedrich Wolter

    2015-07-01

    Full Text Available Learning and planning are powerful AI methods that exhibit complementary strengths. While planning allows goal-directed actions to be computed when a reliable forward model is known, learning allows such models to be obtained autonomously. In this paper we describe how both methods can be combined using an expressive qualitative knowledge representation. We argue that the crucial step in this integration is to employ a representation based on a well-defined semantics. This article proposes the qualitative spatial logic QSL, a representation that combines qualitative abstraction with linear temporal logic, allowing us to represent relevant information about the learning task, possible actions, and their consequences. Doing so, we empower reasoning processes to enhance learning performance beyond the positive effects of learning in abstract state spaces. Proof-of-concept experiments in two simulation environments show that this approach can help to improve learning-based robotics by quicker convergence and leads to more reliable action planning.

  15. From conditional oughts to qualitative decision theory

    Science.gov (United States)

    Pearl, Judea

    1994-01-01

    The primary theme of this investigation is a decision theoretic account of conditional ought statements (e.g., 'You ought to do A, if C') that rectifies glaring deficiencies in classical deontic logic. The resulting account forms a sound basis for qualitative decision theory, thus providing a framework for qualitative planning under uncertainty. In particular, we show that adding causal relationships (in the form of a single graph) as part of an epistemic state is sufficient to facilitate the analysis of action sequences, their consequences, their interaction with observations, their expected utilities, and the synthesis of plans and strategies under uncertainty.

  16. The Qualitative Trajectory Calculus on Networks

    Science.gov (United States)

    Bogaert, Peter; van de Weghe, Nico; Cohn, Anthony G.; Witlox, Frank; de Maeyer, Philippe

    Moving objects are commonly handled using quantitative methods and information. However, in many cases, qualitative information can be more efficient and more meaningful than quantitative information. A lot of research has been done in generating, indexing, modelling and querying network-based moving objects, but little work has been done in building a calculus of relations between these objects in a qualitative way. In this paper, we introduce a formal definition of how to represent and reason about the relative trajectories of pairs of objects moving along a network.

  17. Qualitative research and the modern library

    CERN Document Server

    Goodman, Valeda Dent

    2011-01-01

    Qualitative Research and the Modern Library examines the present-day role and provides suggestions for areas that might be suited to this type of research for the purposes of evaluation. The author discusses how the results from such research might be applied, and the overall impact of using this type of research to inform development of a more user-centred organisation. The book provides a thoughtful look at the implications of using qualitative research to inform decision-making processes within libraries and is written by an author and library researcher with international experience in var

  18. Reasoning about energy in qualitative simulation

    Science.gov (United States)

    Fouche, Pierre; Kuipers, Benjamin J.

    1992-01-01

    While possible behaviors of a mechanism that are consistent with an incomplete state of knowledge can be predicted through qualitative modeling and simulation, spurious behaviors corresponding to no solution of any ordinary differential equation consistent with the model may be generated. The present method for energy-related reasoning eliminates an important source of spurious behaviors, as demonstrated by its application to a nonlinear, proportional-integral controlled. It is shown that such qualitative properties of such a system as stability and zero-offset control are captured by the simulation.

  19. Smartphone log data in a qualitative perspective

    DEFF Research Database (Denmark)

    Ørmen, Jacob; Thorhauge, Anne Mette

    2015-01-01

    into studies of smartphones in everyday life. Through an illustrative study, we explore a more nuanced perspective on what can be considered “log data” and how these types of data can be collected and analysed. A qualitative approach to log data analysis offers researchers new opportunities to situate...... serve as cues to instigate discussion and reflection as well as act as resources for contextualizing and organizing related empirical material. In the discussion, the advantages of a qualitative perspective for research designs are assessed in relation to issues of validity. Further perspectives on the...

  20. Qualitative and quantitative analysis of detonation products

    International Nuclear Information System (INIS)

    Different sampling and different injection method were used during analyzing unknown detonation products in a obturator. The sample analyzed by gas chromatography and gas chromatography/mass spectrum. Qualitative analysis was used with CO, NO, C2H2, C6H6 and so on, qualitative analysis was used with C3H5N, C10H10, C8H8N2 and so on. The method used in the article is feasible. The results show that the component of detonation in the study is negative oxygen balance, there were many pollutants in the detonation products. (authors)

  1. Expert system for transuranic waste assay

    International Nuclear Information System (INIS)

    Transuranic wastes are generated at the Savannah River Site (SRS) as a result of routine production of nuclear materials. These wastes contain Pu-238 and Pu-239 and are placed into lined 55-gallon waste drums. The drums are placed on monitored storage pads pending shipment to the Waste Isolation Pilot Plant in New Mexico. A passive-active neutron (PAN) assay system is used to determine the mass of the radioactive material within the waste drums. Assay results are used to classify the wastes as either low-level or transuranic (TRU). During assays, the PAN assay system communicates with an IBM-AT computer. A Fortran computer program, called NEUT, controls and performs all data analyses. Unassisted, the NEUT program cannot adequately interpret assay results. To eliminate this limitation, an expert system shell was used to write a new algorithm, called the Transuranic Expert System (TRUX), to drive the NEUT program and add decision making capabilities for analysis of the assay results. The TRUX knowledge base was formulated by consulting with human experts in the field of neutron assay, by direct experimentation on the PAN assay system, and by observing operations on a daily basis. TRUX, with its improved ability to interpret assay results, has eliminated the need for close supervision by a human expert, allowing skilled technicians to operate the PAN assay system. 4 refs., 1 fig., 4 tabs

  2. Quantitative comparison of in house irma/ria methods using reagents supplied in bulk with assays based on commercial kits

    International Nuclear Information System (INIS)

    Due to high cost of commercial kit assays, local trials were started to establish low cost immunoassay techniques at MINAR, Multan. First available alternate of commercial kits was the use of matched reagents supplied in bulk by NETRIA through INMOL, Lahore under IAEA assistance. As quality is crucial in RIA estimations this laboratory collected passive quality control data of 50, 51 and 52 in-house assay batches of T3,T4 and TSH to compare with the same number of last Amerlex RIAs(successive lots). A qualitative comparison based on computerized data analysis shows linear correlation between the results of two assay systems with low and acceptable precision in T3 and T4 assays. In TSH assays both systems show high imprecision although Amerlex RIA system is relatively more precise than in-house TSH- IRMA. T3 and T4 assays in both the systems show wide working ranges covering all clinical regions. In TSH, working ranges of both the techniques do not cover all clinical regions. In-house TSH assay excludes below 5.5 mulU/ml, whereas Amerlex excludes levels below 4.5 mulL/ml. This may reduce the clinical efficacy of these tests. Amerlex-M T3 and T4 assays show high negative drift with relatively less between variation, whereas in-house assays show low positive drift with high between batch variation. (author)

  3. Evaluation of the Procleix Ultrio Plus ID NAT assay for detection of HIV 1, HBV and HCV in blood donors

    Directory of Open Access Journals (Sweden)

    Raj Nath Makroo

    2015-01-01

    Full Text Available Introduction: The Procleix Ultrio Plusassay is a new-generation qualitative in vitro nucleic acid amplification test used to screen for human immunodeficiency virus type 1 (HIV-1 RNA, hepatitis C virus (HCV RNA and hepatitis B virus (HBV DNA in blood donors. This study was performed to compare the Procleix Ultrio assay with the new-generation Procleix Ultrio Plus Nucleic Acid Test (NAT assays. Materials and Methods: Ten thousand three hundred and two donor samples were run in parallel for ID NAT using the Procleix Ultrio and the Procleix Ultrio Plus assay. Simultaneously, enzyme-linked immunosorbent assay testing was performed on an EVOLIS Walk away System for HIV, HCV, HBsAg and anti-HBc. Reactive samples were confirmed using polymerase chain reaction. Results: In the 10,302 samples tested during the study period, we identified 15 NAT yields, and all these revealed HBV DNA in the discriminatory assays. Eight of these were exclusive yields from the Ultrio Plus assay and the remaining seven cases were determined as HBV NAT yield, both by the Procleix Ultrio as well as the Ultrio Plus assays, i.e. "Combined" yields. No HCV or HIV 1 yields were detected during the study period by either of two assays. Conclusion: With an overall yield rate of 1 in 687 and an exclusive yield rate of 1 in 1287, the Procleix Ultrio Plus assay proved to be highly sensitive in detecting occult HBV infections.

  4. Harmonization of radiobiological assays: why and how?

    International Nuclear Information System (INIS)

    The International Atomic Energy Agency has made available a technical manual for cytogenetic biodosimetry assays (dicentric chromosome aberration (DCA) and cytokinesis-block micronucleus (CBMN) assays) used for radiation dose assessment in radiation accidents. The International Standardization Organization, which develops standards and guidelines, also provides an avenue for laboratory accreditation, has developed guidelines and recommendations for performing cytogenetic biodosimetry assays. Harmonization of DCA and CBMN assays, has improved their accuracy. Double-blinded inter-laboratory comparison studies involving several networks have further validated DCA and CBMN assays and improved the confidence in their potential use for radiation dose assessment in mass casualties. This kind of international harmonization is lacking for pre-clinical radiobiology assays. The widely used pre-clinical assays that are relatively important to set stage for clinical trials include clonogenic assays, flow-cytometry assays, apoptotic assays, and tumor regression and growth delay assays. However, significant inter-laboratory variations occur with respect to data among laboratories. This raises concerns on the reliability and reproducibility of preclinical data that drives further development and translation. Lack of reproducibility may stem from a variety of factors such as poor scientist training, less than optimal experimental design, inadequate description of methodology, and impulse to publish only the positive data etc. Availability of technical manuals, standard operating procedures, accreditation avenues for laboratories performing such assays, inter-laboratory comparisons, and use of standardized protocols are necessary to enhance reliability and reproducibility. Thus, it is important that radiobiological assays are harmonized for laboratory protocols to ensure successful translation of pre-clinical research on radiation effect modulators to help design clinic trials with

  5. Conducting Qualitative Data Analysis: Managing Dynamic Tensions within

    Science.gov (United States)

    Chenail, Ronald J.

    2012-01-01

    In the third of a series of "how-to" essays on conducting qualitative data analysis, Ron Chenail examines the dynamic tensions within the process of qualitative data analysis that qualitative researchers must manage in order to produce credible and creative results. These tensions include (a) the qualities of the data and the qualitative data…

  6. Understanding Participatory Action Research: A Qualitative Research Methodology Option

    Science.gov (United States)

    MacDonald, Cathy

    2012-01-01

    Participatory Action Research (PAR) is a qualitative research methodology option that requires further understanding and consideration. PAR is considered democratic, equitable, liberating, and life-enhancing qualitative inquiry that remains distinct from other qualitative methodologies (Kach & Kralik, 2006). Using PAR, qualitative features of an…

  7. Insights into Pain: A Review of Qualitative Research

    OpenAIRE

    Osborn, Mike; Rodham, Karen

    2010-01-01

    Qualitative research exposes and explores important aspects of the pain experience that are inaccessible to other approaches.Qualitative work adopts a different epistemological and ontological perspective to quantitative work.Qualitative research is not well established in the field of pain, but is growing.More interpretative engagement with qualitative data is required.

  8. Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester

    Directory of Open Access Journals (Sweden)

    Hampus Sunner

    2015-09-01

    Full Text Available Research on glucuronoyl esterases (GEs has been hampered by the lack of enzyme assays based on easily obtainable substrates. While benzyl d-glucuronic acid ester (BnGlcA is a commercially available substrate that can be used for GE assays, several considerations regarding substrate instability, limited solubility and low apparent affinities should be made. In this work we discuss the factors that are important when using BnGlcA for assaying GE activity and show how these can be applied when designing BnGlcA-based GE assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery of different GEs through an extensive screening of heterologous Genomic and Metagenomic expression libraries.

  9. Identification of irradiated refrigerated pork with the DNA comet assay

    International Nuclear Information System (INIS)

    Food irradiation can contribute to a safer and more plentiful food supply by inactivating pathogens, eradicating pests and by extending shelf-life. Particularly in the case of pork meat, this process could be a useful way to inactivate harmful parasites such as Trichinella and Taenia solium. Ionizing radiation causes damage to the DNA of the cells (e.g. strand breaks), which can be used to detect irradiated food. Microelectrophoresis of single cells ('Comet Assay') is a simple and rapid test for DNA damage and can be used over a wide dose range and for a variety of products. Refrigerated pork meat was irradiated with a 60Co source, Gammacell 220 (A.E.C.L.) installed in IPEN (Sao Paulo, Brazil). The doses given were 0, 1.5, 3.0 and 4.5 kGy for refrigerated samples. Immediately after irradiation the samples were returned to the refrigerator (6 deg. C). Samples were kept in the refrigerator after irradiation. Pork meat was analyzed 1, 8 and 10 days after irradiation using the DNA 'Comet Assay'. This method showed to be an inexpensive and rapid technique for qualitative detection of irradiation treatment

  10. Identification of irradiated refrigerated pork with the DNA comet assay

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, M.M. E-mail: villavic@net.ipen.br; Marin-Huachaca, N.S.; Mancini-Filho, J. E-mail: jmancini@usp.br; Delincee, H.; Villavicencio, A.L.C.H. E-mail: henry.delincee@bfe.uni-karlsruhe.de

    2004-10-01

    Food irradiation can contribute to a safer and more plentiful food supply by inactivating pathogens, eradicating pests and by extending shelf-life. Particularly in the case of pork meat, this process could be a useful way to inactivate harmful parasites such as Trichinella and Taenia solium. Ionizing radiation causes damage to the DNA of the cells (e.g. strand breaks), which can be used to detect irradiated food. Microelectrophoresis of single cells ('Comet Assay') is a simple and rapid test for DNA damage and can be used over a wide dose range and for a variety of products. Refrigerated pork meat was irradiated with a {sup 60}Co source, Gammacell 220 (A.E.C.L.) installed in IPEN (Sao Paulo, Brazil). The doses given were 0, 1.5, 3.0 and 4.5 kGy for refrigerated samples. Immediately after irradiation the samples were returned to the refrigerator (6 deg. C). Samples were kept in the refrigerator after irradiation. Pork meat was analyzed 1, 8 and 10 days after irradiation using the DNA 'Comet Assay'. This method showed to be an inexpensive and rapid technique for qualitative detection of irradiation treatment.

  11. Identification of irradiated refrigerated pork with the DNA comet assay

    Science.gov (United States)

    Araújo, M. M.; Marin-Huachaca, N. S.; Mancini-Filho, J.; Delincée, H.; Villavicencio, A. L. C. H.

    2004-09-01

    Food irradiation can contribute to a safer and more plentiful food supply by inactivating pathogens, eradicating pests and by extending shelf-life. Particularly in the case of pork meat, this process could be a useful way to inactivate harmful parasites such as Trichinella and Taenia solium. Ionizing radiation causes damage to the DNA of the cells (e.g. strand breaks), which can be used to detect irradiated food. Microelectrophoresis of single cells (``Comet Assay'') is a simple and rapid test for DNA damage and can be used over a wide dose range and for a variety of products. Refrigerated pork meat was irradiated with a 60Co source, Gammacell 220 (A.E.C.L.) installed in IPEN (Sa~o Paulo, Brazil). The doses given were 0, 1.5, 3.0 and 4.5kGy for refrigerated samples. Immediately after irradiation the samples were returned to the refrigerator (6°C). Samples were kept in the refrigerator after irradiation. Pork meat was analyzed 1, 8 and 10 days after irradiation using the DNA ``Comet Assay''. This method showed to be an inexpensive and rapid technique for qualitative detection of irradiation treatment.

  12. An ultrafiltration assay for lysyl oxidase

    Energy Technology Data Exchange (ETDEWEB)

    Shackleton, D.R.; Hulmes, D.J. (Univ. of Edinburgh Medical School (England))

    1990-03-01

    A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a (4,5-3H)-lysine-labeled elastin substrate. Enzyme activity is determined by scintillation counting of the ultrafiltrate, after subtraction of radioactivity released in the presence of beta-aminopropionitrile, a specific inhibitor of the enzyme. Conditions are described which optimize both the sensitivity and the efficient use of substrate. The assay shows linear inhibition of activity in up to 1 M urea; hence, as the enzyme is normally diluted in the assay, samples in 6 M urea can be assayed directly, without prior dialysis, and corrected for partial inhibition. Comparable results are obtained when enzyme activity is assayed by ultrafiltration or microdistillation. The assay is simple and convenient and, by using disposable containers throughout, it eliminates the need for time-consuming decontamination of radioactive glassware.

  13. Assay development status report for total cyanide

    Energy Technology Data Exchange (ETDEWEB)

    Simpson, B.C. [Westinghouse Hanford Co., Richland, WA (United States); Jones, T.E.; Pool, K.H. [Pacific Northwest Lab., Richland, WA (United States)

    1993-02-01

    A validated cyanide assay that is applicable to a variety of tank waste matrices is necessary to resolve certain waste tank safety issues and for purposes of overall waste characterization. The target for this effort is an assay with an applicable range of greater than 1,000 ppM (0.10 wt%) total cyanide and a confidence level greater than 80%. Figure 1 illustrates the operating regime of the proposed cyanide assay method. The Assay Development Status Report for Total Cyanide will summarize the past experience with cyanide analyses on-tank waste matrices and will rate the status of the analytical methods used to assay total cyanide (CN{sup {minus}} ion) in the tank waste matrices as acceptable or unacceptable. This paper will also briefly describe the current efforts for improving analytical resolution of the assays and the attempts at speciation.

  14. An ultrafiltration assay for lysyl oxidase

    International Nuclear Information System (INIS)

    A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Enzyme activity is determined by scintillation counting of the ultrafiltrate, after subtraction of radioactivity released in the presence of beta-aminopropionitrile, a specific inhibitor of the enzyme. Conditions are described which optimize both the sensitivity and the efficient use of substrate. The assay shows linear inhibition of activity in up to 1 M urea; hence, as the enzyme is normally diluted in the assay, samples in 6 M urea can be assayed directly, without prior dialysis, and corrected for partial inhibition. Comparable results are obtained when enzyme activity is assayed by ultrafiltration or microdistillation. The assay is simple and convenient and, by using disposable containers throughout, it eliminates the need for time-consuming decontamination of radioactive glassware

  15. Thyroglobulin measurement by highly sensitive assays

    DEFF Research Database (Denmark)

    Giovanella, Luca; Feldt-Rasmussen, Ulla; Verburg, Frederik A;

    2015-01-01

    thyroglobulin (Tg), circulating Tg serves as a biochemical marker of persistent or recurrent disease in the follow-up of DTC. Due to the suboptimal clinical detection rate of older Tg assays endogenous or exogenous thyrotropin (TSH) stimulations are recommended for unmasking occult disease. However, the...... development of new Tg assays with improved analytical sensitivity and precision at low concentrations now allows detection of very low Tg concentrations, reflecting minimal amounts of thyroid tissue, even without the need for TSH stimulation. Even if the use of these assays still has not found its way in...... current clinical guidelines, such assays are now increasingly used in clinical practice. As serum Tg measurement is a technically challenging assay and criteria to define a 'highly sensitive' assay may be different, a good knowledge of the technical difficulties and interpretation criteria is of paramount...

  16. Automated amperometric plutonium assay system

    International Nuclear Information System (INIS)

    The amperometric titration for plutonium assay has been used in the nuclear industry for over twenty years and has been in routine use at the Hanford Engineering Development Laboratory since 1976 for the analysis of plutonium oxide and mixed oxide fuel material for the Fast Flux Test Facility. It has proven itself to be an accurate and reliable method. The method may be used as a direct end point titration or an excess of titrant may be added and a back titration performed to aid in determination of the end point. Due to the slowness of the PuVI-FeII reaction it is difficult to recognize when the end point is being approached and is very time consuming if the current is allowed to decay to the residual value after each titrant addition. For this reason the back titration in which the rapid FeII-CrVI reaction occurs is used by most laboratories. The back titration is performed by the addition of excess ferrous solution followed by two measured aliquots of standard dichromate with measurement of cell current after each addition

  17. Qualitative Research: A Grounded Theory Example and Evaluation Criteria

    OpenAIRE

    Bitsch, Vera

    2005-01-01

    The qualitative research paradigm, although occasionally applied, is not widely discussed in agribusiness and agricultural economics literature. The primary goals of this paper are (a) to present insights into qualitative research approaches and processes by outlining grounded theory as an example of a systematic and rigorous qualitative approach, and (b) to discuss criteria for scientific rigor applicable to qualitative research. In addition, assessing qualitative research is demonstrated by...

  18. Matrix effects of TRU [transuranic] assays using the SWEPP PAN assay system

    International Nuclear Information System (INIS)

    The Drum Assay System (DAS) at the Stored Waste Experimental Pilot Plant (SWEPP) is a second-generation active-passive neutron assay system. It has been used to assay over 5000 208-liter drums of transuranic waste from the Rocky Flats Plant (RFP). Data from these assays have been examined and compared with the assays performed at Rocky Flats, mainly utilize counting of 239Pu gamma rays. For the most part the passive assays are in very good agreement with the Rocky Flats assays. The active assays are strongly correlated with the results of the other two methods, but require matrix-dependent correction factors beyond those provided by the system itself. A set of matrix-dependent correction factors has been developed from the study of the assay results. 3 refs., 4 figs., 3 tabs

  19. Immunofluorescence Assay for Serologic Diagnosis of SARS

    OpenAIRE

    Chan, Paul K. S.; Ng, King-Cheung; Chan, Rickjason C. W.; Lam, Rebecca K. Y.; Chow, Viola C. Y.; Hui, Mamie; Wu, Alan; Lee, Nelson; Yap, Florence H.Y.; Cheng, Frankie W.T.; Joseph J. Y. Sung; Tam, John S

    2004-01-01

    We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

  20. Trypan blue exclusion assay by flow cytometry

    OpenAIRE

    Avelar-Freitas, B.A.; V.G. Almeida; Pinto, M.C.X.; F.A.G. Mourão; A.R. Massensini; O.A. Martins-Filho; E. Rocha-Vieira; G.E.A. Brito-Melo

    2014-01-01

    Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating ce...

  1. Murder and Motivation: A Qualitative Study

    OpenAIRE

    Mirzaei Alavijeh; Mostafavi; Ahmadpanah; Karami Matin; Amoei; Jalilian

    2015-01-01

    Background Murder is considered as the worst crime in all societies. It might be accomplished suddenly based on previous resolution, effect of stimuli, or due to a mental condition. Objectives The present study aimed to determine the reasons and motivations to commit a murder from murderers’ point of view. Patients and Methods It is a qualitative research making use of content anal...

  2. Values in Qualitative and Quantitative Research

    Science.gov (United States)

    Duffy, Maureen; Chenail, Ronald J.

    2008-01-01

    The authors identify the philosophical underpinnings and value-ladenness of major research paradigms. They argue that useful and meaningful research findings for counseling can be generated from both qualitative and quantitative research methodologies, provided that the researcher has an appreciation of the importance of philosophical coherence in…

  3. Doing Qualitative Studies, Using Statistical Reasoning

    DEFF Research Database (Denmark)

    Kristensen, Tore; Gabrielsen, Gorm

    Qualitative studies are associated with interviews, focus groups and observations. We introduce experiments as a way of dealing with such studies. In contrast to the common focus on how many respondents choose a particular behaviour we focus on how much a design affect the individual. This is oft...

  4. Qualität der Eizelle

    Directory of Open Access Journals (Sweden)

    Montag M

    2009-01-01

    Full Text Available Im Rahmen der assistierten Reproduktion ist die Qualität der Eizelle ein entscheidendes Kriterium für den Erfolg einer Kinderwunschbehandlung. Die lichtmikroskopische Untersuchung morphologischer Kriterien stellte lange Zeit die einzige Möglichkeit zur Beurteilung der Eizellqualität dar und hat auch heute noch ihre Berechtigung. In zunehmendem Maße werden auch andere diagnostische Verfahren evaluiert. Neben der chromosomalen bzw. genetischen Integrität, die mit geeigneten Methoden bereits untersucht werden kann, stellt die nukleäre und die zytoplasmatische Reife der Eizellen ein wesentliches Qualitätsmerkmal dar. Bisher stehen insbesondere indirekte Untersuchungsmethoden zur Verfügung, die zum Teil bereits in der klinischen Praxis eingesetzt werden, wie z. B. das Zonaimaging. Neueste Entwicklungen wie die Sauerstoff-Respirationsmessung oder die Genexpressionsanalyse befinden sich noch im Entwicklungsstadium und werden hinsichtlich ihrer Einsatzmöglichkeit im Laboralltag evaluiert. Für einige neue Parameter sind bei verschiedenen Eizellen zum Teil bereits erhebliche Unterschiede erkennbar, die als indirekter Hinweis auf qualitative Unterschiede angesehen werden können. Erst mit genügend sensitiven Untersuchungsmethoden werden die zum Teil geringen Unterschiede zwischen Eizellen verschiedener Qualität erkennbar.

  5. A qualitative evaluation of The European Library

    OpenAIRE

    Klas, Claus-Peter; Τσάκωνας, Γιάννης; Albrechtsen, Hanne; Hansen, Preben

    2007-01-01

    Evaluation of digital libraries assesses their effectiveness, quality and overall impact. In this paper we describe a qualitative evaluation of “The European Library” carried out by six highly qualified evaluators from the area of computer and library science. The findings - mainly usability issues - are presented along the ITF model and suggestions are given to overcome these findings.

  6. Getting Over the Quantitative-Qualitative Debate.

    Science.gov (United States)

    Howe, Kenneth R.

    1992-01-01

    Describes the evolution of the qualitative-quantitative debate, and suggests that educational researchers learn to live with the necessary tensions resulting from accepting elements of each approach. The proposed critical educational research model is illustrated through examples that go beyond a positivist-interpretivist split. (SLD)

  7. Qualitative models of global warming amplifiers

    NARCIS (Netherlands)

    U. Milošević; B. Bredeweg

    2010-01-01

    There is growing interest from ecological experts to create qualitative models of phenomena for which numerical information is sparse or missing. We present a number of successful models in the field of environmental science, namely, the domain of global warming. The motivation behind the effort is

  8. Handbook of Qualitative Research. Second Edition.

    Science.gov (United States)

    Denzin, Norman K., Ed.; Lincoln, Yvonna S., Ed.

    This handbook's second edition represents the state of the art for the theory and practice of qualitative inquiry. It features eight new topics, including autoethnography, critical race theory, applied ethnography, queer theory, and "testimonio"every chapter in the handbook has been thoroughly revised and updated. The book contains:"Preface" (1…

  9. Collecting and Interpreting Qualitative Materials. Third Edition

    Science.gov (United States)

    Denzin, Norman K., Ed.; Lincoln, Yvonna, Ed.

    2007-01-01

    This book is the third volume of the paperback versions of "The SAGE Handbook of Qualitative Research, Third Edition." This portion of the handbook considers the tasks of collecting, analyzing, and interpreting empirical materials, and comprises the Handbook's Parts IV ("Methods of Collecting and Analyzing Empirical Materials") and V ("The Art and…

  10. Developing Qualitative Research Questions: A Reflective Process

    Science.gov (United States)

    Agee, Jane

    2009-01-01

    The reflective and interrogative processes required for developing effective qualitative research questions can give shape and direction to a study in ways that are often underestimated. Good research questions do not necessarily produce good research, but poorly conceived or constructed questions will likely create problems that affect all…

  11. Innovative Data Collection Strategies in Qualitative Research

    Science.gov (United States)

    Onwuegbuzie, Anthony J.; Leech, Nancy L.; Collins, Kathleen M. T.

    2010-01-01

    This article provides an innovative meta-framework comprising strategies designed to guide qualitative data collection in the 21st century. We present a meta-framework comprising strategies for collecting data from interviews, focus groups, observations, and documents/material culture. We present a template for collecting nonverbal data during…

  12. Why Students Procrastinate: A Qualitative Approach

    Science.gov (United States)

    Klingsieck, Katrin B.; Grund, Axel; Schmid, Sebastian; Fries, Stefan

    2013-01-01

    In this study we adopted an impartial view on academic procrastination in order to gain new insights for the development of intervention programs. Following a qualitative approach, we thereby explored antecedents of procrastination by attending to the actual voices and experiences of 29 students. Students' subjective theories were in line…

  13. A Qualitative Acceleration Model Based on Intervals

    Directory of Open Access Journals (Sweden)

    Ester MARTINEZ-MARTIN

    2013-08-01

    Full Text Available On the way to autonomous service robots, spatial reasoning plays a main role since it properly deals with problems involving uncertainty. In particular, we are interested in knowing people's pose to avoid collisions. With that aim, in this paper, we present a qualitative acceleration model for robotic applications including representation, reasoning and a practical application.

  14. Using Qualitative Methods to Study Friendships

    Science.gov (United States)

    Bukowski, William; Lisboa, Carolina

    2005-01-01

    Basic concepts and procedures of qualitative analysis are discussed, especially as they relate to the study of the features, processes, and effects of friendships. The contributions of the previous chapters are presented according to theory and research on friendship as a developmental process.

  15. Facilitating Coherence across Qualitative Research Papers

    Science.gov (United States)

    Chenail, Ronald J.; Duffy, Maureen; St. George, Sally; Wulff, Dan

    2011-01-01

    Bringing the various elements of qualitative research papers into coherent textual patterns presents challenges for authors and editors alike. Although individual sections such as presentation of the problem, review of the literature, methodology, results, and discussion may each be constructed in a sound logical and structural sense, the…

  16. Culturally Competent Qualitative Research with Latino Immigrants

    Science.gov (United States)

    Ojeda, Lizette; Flores, Lisa Y.; Meza, Rocio Rosales; Morales, Alejandro

    2011-01-01

    This article provides recommendations for conducting culturally competent qualitative research with Latino immigrants, a historically exploited group that represents more than half of all U.S. immigrants and is continuously growing. Limited research exists on Latino immigrants despite their large presence in the United States. The authors draw…

  17. The Ethics of Qualitative Nursing Research.

    Science.gov (United States)

    Robley, Lois R.

    1995-01-01

    Ethical issues in qualitative nursing research include the following: what to study, which participants, what methods, how to achieve informed consent, when to terminate interviews and when to probe, when treatment should supersede research, and what and how to document in case studies. (SK)

  18. Preliminary aspects of a qualitative research projection

    OpenAIRE

    Simona Haidau

    2012-01-01

    In this paper are presented preliminary aspects on the design of of qualitative research achieved by nongovernmental organizations in the North-East of Romȃnia. The research aims to identify attitudes, opinions and behaviors to nongovernmental organizations to implement public relations programs and campaigns.

  19. Asian American Career Development: A Qualitative Analysis

    Science.gov (United States)

    Fouad, Nadya A.; Kantamneni, Neeta; Smothers, Melissa K.; Chen, Yung-Lung; Fitzpatrick, Mary; Terry, Sarah

    2008-01-01

    This study used a modified version of consensual qualitative research design to examine how contextual, cultural, and personal variables influence the career choices of a diverse group of 12 Asian Americans. Seven domains of influences on career choices emerged including family, culture, external factors, career goals, role models, work values,…

  20. Safeguards and Non-destructive Assay

    International Nuclear Information System (INIS)

    SCK-CEN's programme on safeguards and non-destructive assay includes: (1) various activities to assure nuclear materials accountancy; (2) contributes to the implementation of Integrated Safeguards measures in Belgium and to assist the IAEA through the Belgian Support Programme; (3) renders services to internal and external customers in the field of safeguards; (4) improves passive neutron coincidence counting techniques for waste assay and safeguards verification measurements by R and D on correlation algorithms implemented via software or dedicated hardware; (5) improves gamma assay techniques for waste assay by implementing advanced scanning techniques and different correlation algorithms; and (6) develops numerical calibration techniques. Major achievements in these areas in 2000 are reported

  1. Detection of irradiated onion by means of the comet assay

    International Nuclear Information System (INIS)

    The ionizing radiations are used as a harmless alternative treatment that it substitutes the employment of chemical treatments, which after their application in the food products can remain residuals not desired that they come to be carcinogenic. With the food irradiation is eliminated microorganisms and the storage time is prolonged, which produces benefits for the Food Industry and the consumers. In many countries the search of sensitive detecting methods of irradiated foods is promoted by the necessity of the assurance of the consumption of foods with nutritional quality and to test directly the radiation processing, for which several techniques have been developed, these are based on the changes that induce the ionizing radiations in the food products. A recommended method is the Comet Assay of DNA, it is approved by the European Committee of Standardization (EN 13784). The DNA molecule is very sensitive to gamma radiations even at low radiation dose, where the modifications produced in the molecule can be monitored for this analytical technique well-known as Comet Assay of DNA or Single Cell Gel Electrophoresis. The objective of the present paper was to evaluate the modifications of the DNA molecule of irradiated onions with the Comet Assay for several dose values, the onions were conserved at environment and refrigeration temperatures. The samples were irradiated in a self-shielding irradiator with 60Co source, dose rate of 20.45 Gy/min and absorbed dose values of 0.5; 0.6; 0.8 and 1.0 kGy. This detection method demonstrates to be one sensitive and quick technique for the qualitative detection of irradiated onions. (author)

  2. Detection of irradiated onion by means of the comet assay

    Energy Technology Data Exchange (ETDEWEB)

    Moreno Alvarez, Damaris L.; Prieto Miranda, Enrique Fco.; Carro Palacio, Sandra [Centro de Aplicaciones Tecnologicas y Desarrollo Nuclear. (CEADEN), Ciudad de La Habana (Cuba)]. E-mail: damaris@ceaden.edu.cu; efprieto@ceaden.edu.cu; Iglesia Enriquez, Isora [Instituto de Investigacion para la Industria Alimenticia (IIIA), Ciudad de La Habana (Cuba)

    2007-07-01

    The ionizing radiations are used as a harmless alternative treatment that it substitutes the employment of chemical treatments, which after their application in the food products can remain residuals not desired that they come to be carcinogenic. With the food irradiation is eliminated microorganisms and the storage time is prolonged, which produces benefits for the Food Industry and the consumers. In many countries the search of sensitive detecting methods of irradiated foods is promoted by the necessity of the assurance of the consumption of foods with nutritional quality and to test directly the radiation processing, for which several techniques have been developed, these are based on the changes that induce the ionizing radiations in the food products. A recommended method is the Comet Assay of DNA, it is approved by the European Committee of Standardization (EN 13784). The DNA molecule is very sensitive to gamma radiations even at low radiation dose, where the modifications produced in the molecule can be monitored for this analytical technique well-known as Comet Assay of DNA or Single Cell Gel Electrophoresis. The objective of the present paper was to evaluate the modifications of the DNA molecule of irradiated onions with the Comet Assay for several dose values, the onions were conserved at environment and refrigeration temperatures. The samples were irradiated in a self-shielding irradiator with {sup 60}Co source, dose rate of 20.45 Gy/min and absorbed dose values of 0.5; 0.6; 0.8 and 1.0 kGy. This detection method demonstrates to be one sensitive and quick technique for the qualitative detection of irradiated onions. (author)

  3. Zur Qualitätsausbildung und Qualitätserhaltung gartenbaulicher Produkte

    OpenAIRE

    Masoud, Alaa Abd El-Gaber Badawi

    2003-01-01

    Qualität wird nach ISO 8402 als Gesamtheit von Eigenschaften und Merkmalen eines Produktes, die sich auf deren Eignung zur Erfüllung festgelegter oder vorausgesetzter Erfordernisse beziehen, definiert. Produkte mit hohem Wassergehalt und großer Atmungsrate können erhebliche Qualitätsbeeinträchtigungen in der Nachernteperiode bis zum Verkauf bzw. Verzehr erleiden. Am Beispiel des Spargels, also junger und physiologisch sehr aktiver Pflanzensprosse, werden äußere und innere Qualitätsmerkmale ...

  4. A radiochemical assay for biotin in biological materials

    International Nuclear Information System (INIS)

    A radiochemical assay for biotin is described. The assay was sensitive to one nanogram and simple enough for routine biotin analyses. The assay yielded results which were comparable to those obtained from a microbiological assay using Lactobacillus plantarum. (author)

  5. Plasmatic and thermographic consequences of local acute irradiation; a qualitative and quantitative analysis in the pig

    International Nuclear Information System (INIS)

    Acute phase reactant proteins associated with thermographic measurements and enzymatic activity assays in plasma were carried out on 39 pigs, following local exposure of the thigh to a collimated source of iridium 192 at doses ranging between 30 and 84 Gy (2 cm depth dose). The inflammatory response after irradiation, from day 1 to day 30 was accompanied by plasma protein changes associated with an elevation of local and general temperatures in irradiated animals. Degenerative processes in muscle led to an increase of plasmatic creatine kinase and lactate-dehydrogenase. Results were developed qualitatively (distribution pattern of proteins, thermographic measurements, enzymatic activities and clinical evolution of the lesions) and qualitatively (plasma level of creatine kinase versus applied radiation doses and pharmalogical treatments)

  6. LT-HSC Methylcellulose Assay

    Science.gov (United States)

    Kerenyi, Marc A.

    2016-01-01

    Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their exceptional ability to divide asymmetrically giving rise to two different kinds of offspring. One daughter cell becomes an LT-HSC itself (self-renews) to maintain the LT-HSC pool, whereas the second daughter cell pursues a differentiation fate to ultimately give rise to terminally differentiated mature blood cells (Orkin and Zon, 2008). Quantification of phenotypic LT-HSCs can be performed by multi-color flow cytometry and the gold standard for assessment of LT-HSC self-renewal and function is competitive bone marrow transplantation (Miller et al., 2008). Although these methods are irreplaceable to determine LT-HSC abundance and functionality, they have their disadvantages and limitations. For example, competitive bone marrow transplantation is typically monitored as a function of peripheral blood donor contribution over 12–16 weeks. While reduced peripheral blood donor contribution by itself signifies impairment in the stem/progenitor cells compartment, it cannot unambiguously discriminate between reduced LT-HSC self-renewal, impaired LT-HSC differentiation or compromised progenitor cell differentiation. Here we describe an LT-HSCs methylcellulose colony-forming assay, as a fast complementary in vitro method to directly assess LT-HSC differentiation capacity. As described in Kerenyi et al. (2013), this technique acts as a powerful tool to differentiate between LT-HSC or progenitor cell differentiation defects.

  7. Acellular comet assay: A tool for assessing variables influencing the alkaline comet assay

    International Nuclear Information System (INIS)

    In this study, an acellular modification to the alkaline comet assay to further evaluate key variables within the assay that may influence the outcome of genotoxicity studies is described. This acellular comet assay can detect differences of 0.2 Gy of 60Co gamma-ray radiation between 0 and 1 Gy and differences of 1 Gy between 0 and 8 Gy; thus, this assay is applicable for a wide range of DNA damage levels. It is also shown that DNA damage from different radiation energies was not significantly different from 60Co gamma-ray. This assay displayed a statistical increase in DNA damage due to uncontrolled exposure to natural light; however, the slope of the dose-response curve for light-exposed samples was similar to that for samples protected from light. A comparison of the alkaline comet assay with the acellular comet assay allowed for the intrinsic repair capacity of the alkaline comet assay to be quantified. (authors)

  8. Development of an upconverting chelate assay

    Science.gov (United States)

    Xiao, Xudong; Haushalter, Jeanne P.; Kotz, Kenneth T.; Faris, Gregory W.

    2005-04-01

    We report progress on performing a cell-based assay for the detection of EGFR on cell surfaces by using upconverting chelates. An upconversion microscope has been developed for performing assays and testing optical response. A431 cells are labeled with europium DOTA and imaged using this upconverting microscope.

  9. A bioluminescent assay for measuring glucose uptake.

    Science.gov (United States)

    Valley, Michael P; Karassina, Natasha; Aoyama, Natsuyo; Carlson, Coby; Cali, James J; Vidugiriene, Jolanta

    2016-07-15

    Identifying activators and inhibitors of glucose uptake is critical for both diabetes management and anticancer therapy. To facilitate such studies, easy-to-use nonradioactive assays are desired. Here we describe a bioluminescent glucose uptake assay for measuring glucose transport in cells. The assay is based on the uptake of 2-deoxyglucose and the enzymatic detection of the 2-deoxyglucose-6-phosphate that accumulates. Uptake can be measured from a variety of cell types, it can be inhibited by known glucose transporter inhibitors, and the bioluminescent assay yields similar results when compared with the radioactive method. With HCT 116 cells, glucose uptake can be detected in as little as 5000 cells and remains linear up to 50,000 cells with signal-to-background values ranging from 5 to 45. The assay can be used to screen for glucose transporter inhibitors, or by multiplexing with viability readouts, changes in glucose uptake can be differentiated from overall effects on cell health. The assay also can provide a relevant end point for measuring insulin sensitivity. With adipocytes and myotubes, insulin-dependent increases in glucose uptake have been measured with 10- and 2-fold assay windows, respectively. Significant assay signals of 2-fold or more have also been measured with human induced pluripotent stem cell (iPSC)-derived cardiomyocytes and skeletal myoblasts. PMID:27130501

  10. Radioreceptor assay: theory and applications to pharmacology

    International Nuclear Information System (INIS)

    The aim of the first part of this work is to present the theory of the radioreceptor assay and to compare it to the other techniques of radioanalysis (radioimmunoassay, competitive protein binding assays). The technology of the radioreceptor assay is then presented and its components (preparation of the receptors, radioligand, incubation medium) are described. The analytical characteristics of the radioreceptor assay (specificity, sensitivity, reproductibility, accuracy) and the pharmacological significance of the results are discussed. The second part is devoted to the description of the radioreceptor assays of some pharmacological classes (neuroleptics, tricyclic antidepressants, benzodiazepines, β-blockers, anticholinergic drugs) and to their use in therapeutic drug monitoring. In conclusion, by their nature, radioreceptor assays are highly sensitive, reliable, precise, accurate and simple to perform. Their chief disadvantage relates to specificity, since any substance having an appreciable affinity to the receptor site will displace the specifically bound radioligand. Paradoxically in some cases, this lack of specificity may be advantageous in that it allows for the detection of not only the apparent compound but of active metabolites and endogenous receptor agonists as well and in that radioreceptors assays can be devised for a whole pharmacological class and not only for one drug as it is the case for classical physico-chemical techniques. For all these reasons future of radioreceptor assay in pharmacology appears promising

  11. Assessing sediment contamination using six toxicity assays

    Directory of Open Access Journals (Sweden)

    Allen G. BURTON Jr.

    2001-08-01

    Full Text Available An evaluation of sediment toxicity at Lake Orta, Italy was conducted to compare a toxicity test battery of 6 assays and to evaluate the extent of sediment contamination at various sediment depths. Lake Orta received excessive loadings of copper and ammonia during the 1900’s until a large remediation effort was conducted in 1989-90 using lime addition. Since that time, the lake has shown signs of a steady recovery of biological communities. The study results showed acute toxicity still exists in sediments at a depth of 5 cm and greater. Assays that detected the highest levels of toxicity were two whole sediment exposures (7 d using Hyalella azteca and Ceriodaphnia dubia. The MicrotoxR assay using pore water was the third most sensitive assay. The Thamnotox, Rototox, Microtox solid phase, and Seed Germination-Root Elongation (pore and solid phase assays showed occasional to no toxicity. Based on similarity of responses and assay sensitivity, the two most useful assays were the C. dubia (or H. azteca and Microtox pore water. These assays were effective at describing sediment toxicity in a weight-of-evidence approach.

  12. Simple UV spectrophotometric assay of Clarithromycin

    Directory of Open Access Journals (Sweden)

    Dr. Safila Naveed

    2014-09-01

    Full Text Available Clarithromycin belongs to semi-synthetic macrolide antibiotic class of drugs that inhibits bacterial protein synthesis.. Our aim of study is to develop a efficient least time consuming and simple spectrophotometric method for the assay of clarithromycin. Comparision of assay of five different brands of clarithromycin (klaricid,klaribact,rithmo,clariteck,E-clark available in public medical store of Karachi, Pakistan has also been done. The assay is based on the ultraviolet UV absorbance maxima at about 210nm wavelength of mefenamic acid, water is used as solvent. A sample of drug was dissolved in water to produce a solution containing mefenamic acid. Similarly, a sample of ground tablets of different brand were dissolved in water and various dilutions were made. The absorbance of sample preparation was measured at 210nm against the solvent blank and the assay was determined by comparing with the absorbance of available brand. Our results reveals that among all the five brands of clarithromycin (klaricid,klaribact,rithmo,clariteck,E-clark Klaribact shows highest percentage assay i.e 115.3846%. Klaricid and Claritecl shows percentage assay of 107.693%. Rithmo shows a percent assay of 92.307% while E-clark shows lowest value for percentage assay 84.6153%

  13. Practical radiochromatographic assay for cholesterol epoxide hydrase

    International Nuclear Information System (INIS)

    A method for the assay of cholesterol epoxide hydrase activity is described. The assay involves the thin-layer chromatographic separation and quantitation of radiolabeled cholestan-3β,5α,6α-epoxide and its major hydration product, cholestan-3β,5α,6β-triol. Radiochromatographic scanning is employed to quantitate the reaction. The procedure is sensitive, rapid, and nondestructive

  14. Qualitative Psychology Nexus, Vol. IX: Beyond Text: Video and other Medium Use in Qualitative Research

    OpenAIRE

    Schweizer, Karin; Feliz Murias, Tiberio; Huber, Günter L.; Almazán Moreno, Lorenzo; Ortega Tudela, Juana María; Ortiz Colón, Ana María; Demuth, Carolin; Gento, Samuel; Domínguez, María-C.; Medina, Antonio; González, Raúl; Drews, Johanna; Kröner, Stephan; Ricoy Lorenzo, María Carmen; Feliz Ricoy, Sálvora

    2012-01-01

    The 10th Annual Workshop of the Center for Qualitative Psychology "Beyond text: Video and other medium use in qualitative research" took place in February 20th- 22nd 2009 in Weingarten, Germany. With this workshop we focused on video analyses and related forms of gathering data for content analyses. The topics of research were discussion groups as a methodological approach, a comparative study of mother-infant-interactions, the evaluation of an international Master course, forms a...

  15. Qualitative Psychology Nexus, Vol. IV: Areas of Qualitative Psychology — Special Focus on Design

    OpenAIRE

    2005-01-01

    This volume contains the proceedings of the fourth annual meeting of Qualitative Psychology in Blaubeuren (near Ulm, Germany) Oct., 22-24, 2003 organized by the Center for Qualitative Psychology (Tübingen, Germany). The question of Research Design was chosen as the central topic of the conference. Researchers from different professions took part. The range of experience of the participants was very heterogeneous: Beginning with young researchers, different levels of expertise were represe...

  16. Qualitative and quantitative evaluation of donkeys responses to immunization by rabbits' IgG

    International Nuclear Information System (INIS)

    In this study two apparently healthy donkeys were immunized with highly pure rabbit's 1gG using a revised protocol. Qualitative test using the same immuno gen was done as a primary test to eva lute the immune system response. However, the same 1gG was iodinated with 125I using chloramine T method and the labeled 1gG was used to quantitatively study the immune response. The two donkeys showed good response with the younger one having the best response. The obtained donkey anti rabbit sera was used as separating agent for RIA assay for human PRL. (Author)

  17. Comparison of real-time and quantitative polymerase chain reaction assays in detection of cytomegalovirus DNA in clinical specimens

    International Nuclear Information System (INIS)

    To compare the real-time (RT) and qualitative (Q) polymerase chain reaction (PCR) assays for detection of Cytomegalovirus (CMV) DNA. The study took place in the Department of Microbiology, Erciyes University, Kayseri and in Iontek Laboratory, Istanbul, Turkey, from August to December 2006. One hundred and seven clinical specimens from 67 patients were included in the study. Cytomegalovirus DNA was investigated using RT-PCR kit (Fluorion Iontek, Turkey) and Q-PCR kit (Fluorion Iontek, Turkey). Deoxyribonucleic acid sequencing was applied to the samples that yielded discrepant results in both assays. Mac Nema's Chi Square test was used for statistical analysis. Of the specimens, 27 were found positive with both assays: 9 with only RT-PCR, and 11 with only Q-PCR assay. Both assays were found negative in 60 of the specimens. There was a good agreement between the 2 assays in 87(81.3%) of the specimens. There was no statistical significant difference between the assays (p>0.05). Two of the 11 samples that RT-PCR negative Q-PCR positive, and 3 of 9 samples that RT-PCR positive Q-PCR negative were found to be CMV DNA positive by DNA sequencing. A good level of concordance between RT-PCR and Q-PCR assays for CMV DNA detection has been found. (author)

  18. Evaluation of a Real-Time PCR Assay Using the LightCycler System for Detection of Parvovirus B19 DNA

    OpenAIRE

    Buller, Richard S.; Storch, Gregory

    2004-01-01

    We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.

  19. Assays for Determination of Protein Concentration.

    Science.gov (United States)

    Olson, Bradley J S C

    2016-01-01

    Biochemical analysis of proteins relies on accurate quantification of protein concentration. Detailed in this appendix are some commonly used methods for protein analysis, e.g., Lowry, Bradford, bicinchoninic acid (BCA), UV spectroscopic, and 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) assays. The primary focus of this report is assay selection, emphasizing sample and buffer compatibility. The fundamentals of generating protein assay standard curves and of data processing are considered, as are high-throughput adaptations of the more commonly used protein assays. Also included is a rapid, inexpensive, and reliable BCA assay of total protein in SDS-PAGE sample buffer that is used for equal loading of SDS-PAGE gels. © 2016 by John Wiley & Sons, Inc. PMID:27248579

  20. Probabilistic interpretation of radioactive waste assay

    International Nuclear Information System (INIS)

    The non-destructive assay of radioactive wastes from nuclear power plants and fuel cycle facilities is required for assessing the disposal risks. Such assay is influenced by two distinct facts: the statistical uncertainty of the measurement and the spatial uncertainty due to the random or at least unknown spatial distribution of the assayed material in a waste container. In this paper a probabilistic interpretation procedure is presented for a single-detector assay system of nuclear waste by one-shot passive gamma technique. The key parameter for this study, the average escape probability for photons, has been obtained by a specific-purpose Monte Carlo code, MCRW. The code has been developed to simulate the entire pulse height spectral responses for point sources located within the compartment which is the basic idea describing the degree of spatial homogeneity of the nuclear and matrix materials. The methodology presented here can be extended to actual radioactive waste assay

  1. The haemolytic antibody isotope release (HAIR) assay; an efficient alternative technique to conventional plaque assays

    International Nuclear Information System (INIS)

    The haemolytic antibody isotope release (HAIR) assay quantitates antibody production by splenic antibody-producing cells by lysis of chromium-51-labelled sheep red blood cells. The amount of antibody quantitated by the HAIR assay directly correlates with the number of antibody-producing cells measured by a conventional plaque assay. The HAIR assay is an easy, sensitive, and reproducible technique that is especially useful when large numbers of animals are required for testing. (author)

  2. Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study

    OpenAIRE

    Buch Karl; Peters Tanja; Nawroth Thomas; Sänger Markus; Schmidberger Heinz; Langguth Peter

    2012-01-01

    Abstract For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well-plates, assays can be performed semi-automatically with a microplate reader. Survival, the calc...

  3. Biomonitoring of genotoxic risk in radar facility workers: comparison of the comet assay with micronucleus assay and chromatid breakage assay

    International Nuclear Information System (INIS)

    Genotoxic risks of occupational exposure in a radar facility were evaluated by using alkaline comet assay, micronucleus assay and chromatid breakage assay on peripheral blood leukocytes in exposed subjects and corresponding controls. Results show that occupational exposure to microwave radiation correlates with an increase of genome damage in somatic cells. The levels of DNA damage in exposed subjects determined by using alkaline comet assay were increased compared to control and showed interindividual variations. Incidence of micronuclei was also significantly increased compared to baseline control values. After short exposure of cultured lymphocytes to bleomycin, cells of occupationally exposed subjects responded with high numbers of chromatid breaks. Although the level of chromosome damage generated by bleomycin varied greatly between individuals, in exposed subjects a significantly elevated number of chromatid breaks was observed. Our results support data reported in literature indicating that microwave radiation represents a potential DNA-damaging hazard. Alkaline comet assay is confirmed as a sensitive and highly reproducible technique for detection of primary DNA damage inflicted in somatic cells. Micronucleus assay was confirmed as reliable bio-markers of effect and chromatid breakage assay as sensitive bio-marker of individual cancer susceptibility. The results obtained also confirm the necessity to improve measures and to perform accurate health surveillance of individuals occupationally exposed to microwave radiation

  4. Ensuring validity in qualitative international business research

    DEFF Research Database (Denmark)

    Andersen, Poul Houman; Skaates, Maria Anne

    2002-01-01

    The purpose of this paper is to provide an account of how the validity issue related to qualitative research strategies within the IB field may be grasped from an at least partially subjectivist point of view. In section two, we will first assess via the aforementioned literature review the extent...... to which the validity issue has been treated in qualitative research contributions published in six leading English-language journals which publish IB research. Thereafter, in section three, we will discuss our findings and relate them to (a) various levels of a research project and (b) the existing...... literature on potential validity problems from a more subjectivist point of view. As a part of this step, we will demonstrate that the assumptions of objectivist and subjectivist ontologies and their corresponding epistemologies merit different canons for assessing research validity. In the subsequent...

  5. Qualitative research ethics on the spot

    DEFF Research Database (Denmark)

    Sørensen, Nelli Øvre; Øye, Christine; Glasdam, Stinne

    2015-01-01

    research ethical guidelines related to informed consent and doing no harm. Third, the article argues for the importance of having research ethical guidelines and review boards to question and discuss the possible ethical dilemmas that occur in qualitative research. Discussion and conclusion: Research......Abstract Background: The increase in medical ethical regulations and bureaucracy handled by institutional review boards and healthcare institutions puts the researchers using qualitative methods in a challenging position. Method: Based on three different cases from three different research studies......, the article explores and discusses research ethical dilemmas. Objectives and ethical considerations: First, and especially, the article addresses the challenges for gatekeepers who influence the informant’s decisions to participate in research. Second, the article addresses the challenges in following...

  6. Troubling Methods in Qualitative Inquiry and Beyond

    DEFF Research Database (Denmark)

    Tanggaard, Lene

    2013-01-01

    This present paper troubles and literally ‘shakes’ the idea of methods as the founding ground of qualitative inquiry. It does so by addressing the real-time messiness of research and the retrospective character of research reports. While the paper is not as such opposed to methods, it does suggest...... that many actual research practices do not follow defined and regular plans as the terminology of methods inclines. However, rather than seeing the messiness as a bias to be eliminated, a more constructive approach is suggested. With the intention of inviting more creative and thought-provoking research...... suggestion is that these open-ended and flexible strategies allow for an innovative approach to the development of a qualitative psychology while also serving to trouble (at least for a moment) the current popularity of methods in research....

  7. Rigor in Qualitative Supply Chain Management Research

    DEFF Research Database (Denmark)

    Goffin, Keith; Raja, Jawwad; Claes, Björn;

    2012-01-01

    Purpose – The purpose of this paper is to share the authors' experiences of using the repertory grid technique in two supply chain management studies. The paper aims to demonstrate how the two studies provided insights into how qualitative techniques such as the repertory grid can be made more...... rigorous than in the past, and how results can be generated that are inaccessible using quantitative methods. Design/methodology/approach – This paper presents two studies undertaken using the repertory grid technique to illustrate its application in supply chain management research. Findings – The paper......, reliability, and theoretical saturation. Originality/value – It is the authors' contention that the addition of the repertory grid technique to the toolset of methods used by logistics and supply chain management researchers can only enhance insights and the building of robust theories. Qualitative studies...

  8. Handling knowledge on osteoporosis - a qualitative study

    DEFF Research Database (Denmark)

    Huniche, Lotte; Brixen, Kim; Sahota, Opinder;

    2013-01-01

    Scand J Caring Sci; 2012 Handling knowledge on osteoporosis - a qualitative study The aim of this qualitative study was to increase understanding of the importance of osteoporosis information and knowledge for patients' ways of handling osteoporosis in their everyday lives. Interviews were...... performed with 14 patients recruited from two English university hospitals and 12 patients from a Danish university hospital. Critical psychology was used as a theoretical framework for the data analysis, which aimed at shedding light on patients' ways of conducting everyday life with osteoporosis. The...... themes that emerged from the analysis showed that life conditions influenced the way in which risk, pain and osteoporosis were handled. Everyday life was also influenced by patients' attitude to treatment. The patients who were experiencing emotional difficulties in handling osteoporosis were not those...

  9. Qualitätsmanagementsysteme - Teil 1

    Science.gov (United States)

    Seghezzi, Hans-Dieter; Wasmer, René

    Medizinprodukte haben grundsätzlich hohen Sicherheitskriterien zu genügen, zuverlässig zu funktionieren und dadurch den erwarteten Gesundheitsschutz und Patientennutzen zu gewährleisten. Dies erfordert schon beim Hersteller ein gut funktionierendes und systematisches Qualitätsmanagement welches dafür bürgt, dass bereits zum Zeitpunkt der Inverkehrbringung neuer Produkte die Konformität mit den Anforderungen besteht und nachweisbar ist. In den 90er Jahren sind insbesondere in Europa die anzuwendenden technischen Regeln für den Bereich der Medizinprodukte spürbar harmonisiert worden. Der Gesetzgeber hat dabei die Anwendung von Qualitätsmanagementsystemen stark gefördert und in den gesetzlichen Vorgaben verankert. Eigenverantwortung und Eigenkontrolle haben dadurch bei der Herstellung von Medizinprodukten höhere Bedeutung erlangt und sind unabdingbar wenn es um die erfolgreiche Marktzulassung geht.

  10. Qualitative versus Quantitative Research in Marketing

    Directory of Open Access Journals (Sweden)

    Russel W. Belk

    2013-04-01

    Full Text Available It is ironic that at a time when wehave more quantitative data aboutconsumers than ever before – so-called “bigdata,” scanner data, loyalty programpurchase histories, trails of Internet searchesand social media activity, and much more –that businesses nevertheless increasinglydesire qualitative information. I am nottalking about the ubiquitous focus groupswhich have long been a staple in exploratorymarketing and advertising research. Suchfocus groups continue to be used, but theyare one of the weakest techniques inqualitative research. The sort of qualitativeconsumer research I have in mind insteadincludes ethnography, netnography, videography,qualitative data mining, projectivemethods, various types of observation, anddepth interviews. What these techniquespotentially provide is more naturalistic insight into how individual consumers andgroups of consumers behave in everyday life.

  11. [Qualitative research: which priority for scientific journals?].

    Science.gov (United States)

    Rodella, Stefania

    2016-04-01

    Quantitative and qualitative approaches in scientific research should not be looked at as separate or even opposed fields of thinking and action, but could rather offer complementary perspectives in order to build appropriate answers to increasingly complex research questions. An open letter recently published by the BMJ and signed by 76 senior academics from 11 countries invite the editors to reconsider their policy of rejecting qualitative research on the grounds of low priority and challenge the journal to develop a proactive, scholarly and pluralistic approach to research that aligns with its stated mission. The contents of the letter, the many voices raised by almost fifty rapid responses and the severe but not closed responses of the editors outline a stimulating debate and hopefully prelude some "change in emphasis", ensuring that all types of research relevant to the mission of the BMJ (as well as other core journals) are considered for publication and providing an evolving landmark for scientific and educational purposes. PMID:27093324

  12. Using qualitative maps to direct reactive robots

    Science.gov (United States)

    Bertin, Randolph; Pendleton, Tom

    1992-01-01

    The principal advantage of mobile robots is that they are able to go to specific locations to perform useful tasks rather than have the tasks brought to them. It is important therefore that the robot be used to reach desired locations efficiently and reliably. A mobile robot whose environment extends significantly beyond its sensory horizon must maintain a representation of the environment, a map, in order to attain these efficiency and reliability requirements. We believe that qualitative mapping methods provide useful and robust representation schemes and that such maps may be used to direct the actions of a reactively controlled robot. In this paper we describe our experience in employing qualitative maps to direct, through the selection of desired control strategies, a reactive-behavior based robot. This mapping capability represents the development of one aspect of a successful deliberative/reactive hybrid control architecture.

  13. Improved benzodiazepine radioreceptor assay using the MultiScreen (R) Assay System

    NARCIS (Netherlands)

    Janssen, MJ; Ensing, K; de Zeeuw, RA

    1999-01-01

    In this article, an improved benzodiazepine radioreceptor assay is described, which allows substantial reduction in assay time, The filtration in this method was performed by using the MultiScreen(R) Assay System. The latter consists of a 96-well plate with glass fibre filters sealed at the bottom,

  14. A Qualitative Exploration of Oral Communciations Apprehension

    OpenAIRE

    Byrne, Marann; Flood, Barbara; Shanahan, Dan

    2012-01-01

    Prior research has identified communication apprehension, or fear of communicating, as a major factor which inhibits an individual’s willingness to communicate and his/her capability to develop effective communication skills. While many prior studies have measured oral communication apprehension of students, there has been little qualitative exploration of the phenomenon. This study was conducted by interviewing first-year business and accounting students at a higher education institution in ...

  15. Evaluating Qualitative Measures for Effective Website Design

    OpenAIRE

    Dr. A.A.Chari; G. Sreedhar; Dr. V.V.Venkata Ramana

    2010-01-01

    The primary goal of this paper is to investigate and evaluate qualitative measures for improving the website design of various universities in India. The website of each university is scanned using W3C guidelines. The parameters such as download time, website size, broken links, etc., are considered in the procedure. Different kinds of tools are used to examine the components of website. These tools include: W3C Link Checker, W3C Markup Validation Service, Webpage Analyzer and Website Extract...

  16. Parenting Coordinators' Practices Recommendations: A Qualitative Study

    OpenAIRE

    Hirsch, Barbara Phyllis

    2016-01-01

    This qualitative study used a phenomenological approach to understand the experiences of seven parenting coordinators in using parenting coordination practices that they have found to be effective and would recommend to other parenting coordinators to achieve the following goals: educating parents, increasing the quality of parenting and co-parenting, managing conflict, and involving children and other family members in the process of parenting coordination. Data were collected with semi-str...

  17. Qualitative Forschung in der kanadischen Psychologie

    OpenAIRE

    Rennie, David L.; Watson, Kimberly D.; Monteiro, Althea

    2000-01-01

    Ausgehend von einer ausführlichen Schilderung des Erstautors zu seiner Situation als qualitativer Forscher in einem großen kanadischen Psychologie-Department wurden kanadische qualitative Forscher(innen) unterschiedlicher disziplinärer Herkunft eingeladen, per E-Mail einige Fragen zur Lage der qualitativen Forschung in Kanada zu beantworten. Eine thematische Analyse der eingegangenen Antworten ergab, daß Psycholog(inn)en, die in Abteilungen arbeiten, die kunst- oder naturwissenschaftlichen Fa...

  18. The qualitative criterion of transient angle stability

    OpenAIRE

    Lyu, R; Xue, Y.; Xue, F; Wu, Qiuwei; Yang, Guangya; Zhou, H; Ju, P.

    2015-01-01

    In almost all the literatures, the qualitative assessment of transient angle stability extracts the angle information of generators based on the swing curve. As the angle (or angle difference) of concern and the threshold value rely strongly on the engineering experience, the validity and robust of these criterions are weak. Based on the stability mechanism from the extended equal area criterion (EEAC) theory and combining with abundant simulations of real system, this paper analyzes the crit...

  19. A qualitative survey of regression testing practices

    OpenAIRE

    Engström, Emelie; Runeson, Per

    2010-01-01

    Aim: Regression testing practices in industry have to be better understood, both for the industry itself and for the research community. Method: We conducted a qualitative industry survey by i) running a focus group meeting with 15 industry participants and ii) validating the outcome in an on line questionnaire with 32 respondents. Results: Regression testing needs and practices vary greatly between and within organizations and at different stages of a project. The importance and challenges o...

  20. Motivation in the Gym: A Qualitative Approach

    OpenAIRE

    Cloes, Marc

    2004-01-01

    Students’ motivation is a major concern of physical education teachers as well as it interest the others educators. Understanding of that complex variable increased during the last two decades. In the physical education domain, many studies were focused on isolated variables related to motivation and on their relationship with pupils’ behaviours. More recently, qualitative researches were conducted to enter in the class specificity and to integrate the complexity and diversity of the variable...

  1. Environmental Risk Communication through Qualitative Risk Assessment

    OpenAIRE

    Sabre J. Coleman; Zalk, David M.

    2014-01-01

    Environmental analysts are often hampered in communicating the risks of environmental contaminants due to the myriad of regulatory requirements that are applicable. The use of a qualitative, risk-based control banding strategy for assessment and control of potential environmental contaminants provides a standardized approach to improve risk communication. Presented is a model that provides an effective means for determining standardized responses and controls for common environmental issues b...

  2. Validity and reliability in qualitative researches

    OpenAIRE

    Mohammad Abbaszadeh

    2012-01-01

    Abstract   Today science world changes sharply in a high speed. In a way that in all areas such as research methods these changes are obvious. Although concepts such as validity and reliability in Positivist methodology have been used with distinct and general rules, in post positivist period in which they have paid much more attention to qualitative research, these principles have lost their general form. In one hand some thinkers stress on applying common validity and reliability quantitati...

  3. Qualitative Research in Communication. Introductory remarks

    OpenAIRE

    Mireia Fernández-Ardèvol; Corina Daba-Buzoianu; Loredana Ivan

    2013-01-01

    The 20th century’s epistemological turn in social sciences (Denzin & Lincoln, 1994; Punch, 2013) acknowledged the importance of qualitative research methods. The need for this turn was also pointed out by Habermas (1979), who noticed that the way data was collected in social sciences affected the analysis and data interpretation. Research gained a comprehensive character and proposed a phenomenological approach of reality (Guba & Lincoln, 1994; Willig, 2008; Lindlof & Taylor, 2011). Nowadays,...

  4. Qualitative research methods in Software Engineering

    OpenAIRE

    Patrick Anya; Gerarld Smith

    2014-01-01

    In the investigation of Software Engineering coexist two different research methods: 1) the quantitative that try to measure and analyze the casual relationship between the variables in a frame with free values, and 2) the qualitative that examine the creation process of meanings from which is generated new theorems or improve. Apply this methods separately in the research in software engineer makes evident that obtain results are incomplete and so is difficult to choose definitively wit whic...

  5. Measuring poverty using qualitative perceptions of welfare

    OpenAIRE

    Pradhan, Menno; Ravallion, Martin

    1998-01-01

    The authors show how subjective poverty lines can be derived using simple qualitative assessments of perceived consumption adequacy, based on a household survey. Respondents were asked whether their consumption of food, housing, and clothing was adequate for their family's needs. The author's approach, by identifying the subjective poverty line without the usual"minimum-income question,"offers wide applications in developing country settings. They implement it using survey data for Jamaica an...

  6. Living with Chronic Pancreatitis: A qualitative study.

    OpenAIRE

    CRONIN, PATRICIA; Begley, Cecily

    2013-01-01

    PUBLISHED OBJECTIVE: Recent literature acknowledges the impact of this progressive and debilitating disease on psychological and social well-being, but the plight of those with chronic pancreatitis remains unknown and hidden. The aim of this study was to develop an understanding of what it means to live with chronic pancreatitis. DESIGN: Qualitative study based on philosophical hermeneutics using multiple unstructured interviews. PARTICIPANTS: Fourteen people with chronic...

  7. Qualitative Forschung und Professionalisierung sozialer Arbeit

    OpenAIRE

    Ackermann, Friedhelm

    1995-01-01

    In einer Reihe neuerer Veröffentlichungen (vgl. zur Übersicht Kraimer 1991) wird vermehrt die Anstrengung unternommen, qualitative Forschungsmethoden für die Soziale Arbeit mit dem Ziel fruchtbar zu machen, die Perspektiven des von Sozialer Arbeit betroffenen Klientels aufzuzeigen und durch die Rekonstruktion der "Lebenskonstruktionen" (Bude) von Subjekten in unterschiedlichen Lebenswelten vorhandene Kompetenzen aufzudecken und Ansatzpunkte für die Soziale Arbeit zu entwickeln. Haben sich qua...

  8. Qualitative Research in OM: Criteria for evaluation

    OpenAIRE

    Martinez, Veronica; Albores, Pavel

    2003-01-01

    The paper presents a reference set of research-quality evaluation criteria for qualitative research in Operations Management. The typical research process is presented and enhanced by making explicit the role of the criteria in both the design and evaluation phases. The paper highlights the importance of having such criteria not only to strengthen the research outcome, but also as means of control and self-correction during the research process. This paper concludes with a r...

  9. Qualitative and quantitative methods in health research

    OpenAIRE

    2009-01-01

    Introduction Research in the area of health has been traditionally dominated by quantitative research. However, the complexity of ill-health, which is socially constructed by individuals, health personnel and health authorities have motivated the search for other forms to approach knowledge. Aim To discuss the complementarities of qualitative and quantitative research methods in the generation of knowledge. Contents The purpose of quantitative research is to measure the magnitude of an event,...

  10. Measuring attitudes: quantitative and qualitative methods

    OpenAIRE

    Carrasco, Ja; Lucas, K

    2015-01-01

    In this paper, we present the discussion and main findings from the ISCTSC Workshop B3, which focused on measuring the role of attitudes and perceptions in people’s travel choices. The paper considers various issues concerning the design and use of quantitative and qualitative approaches in the measurement of attitudes and perceptions within travel surveys. It first identifies the importance of measuring the underlying psychological motivations for people’s travel choices and behaviors and pr...

  11. Antioxidant activity evaluation by physiologically relevant assays based on haemoglobin peroxidase activity and cytochrome c-induced oxidation of liposomes.

    Science.gov (United States)

    Mot, Augustin C; Bischin, Cristina; Muresan, Bianca; Parvu, Marcel; Damian, Grigore; Vlase, Laurian; Silaghi-Dumitrescu, Radu

    2016-06-01

    Two new protocols for exploring antioxidant-related chemical composition and reactivity are described: one based on a chronometric variation of a haemoglobin ascorbate peroxidase assay and one based on cytochrome c-induced oxidation of lecithin liposomes. Detailed accounts are given on their design, application, critical correlations with established methods and mechanisms. These assays are proposed to be physiologically relevant and bring new information regarding a real sample, both qualitative and quantitative. The well-known assays used for evaluation of antioxidant (re)activity are revisited and compared with these new methods. Extracts of the Hedera helix L. are examined as test case, with focus on seasonal variation and on leaf, fruit and flower with respect to chromatographic, spectroscopic and reactivity properties. According to the set of assays performed, winter are the most antioxidant, followed by summer leaves, and then by flowers and fruits. PMID:26208459

  12. Design of Qualitative HRA Database Structure

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seunghwan; Kim, Yochan; Choi, Sun Yeong; Park, Jinkyun; Jung, Wondea [KAERI, Daejeon (Korea, Republic of)

    2015-05-15

    HRA DB is to collect and store the data in a database form to manage and maintain them from the perspective of human reliability analysis. All information on the human errors taken by operators in the power plant should be systematically collected and documented in its management. KAERI is developing the simulator-based HRA data handbook. In this study, the information required to store and manage the data necessary to perform an HRA as to store the HRA data to be stored in the handbook is identified and summarized. Especially this study is to summarize the collection and classification of qualitative data as the raw data to organize the data required to draw the HEP and its DB process. Qualitative HRA DB is a storehouse of all sub-information needed to receive the human error probability for Pasa. In this study, the requirements for structural design and implementation of qualitative HRA DB must be implemented for HRA DB were summarized. The follow-up study of the quantitative HRA DB implementation should be followed to draw the substantial HEP.

  13. Design of Qualitative HRA Database Structure

    International Nuclear Information System (INIS)

    HRA DB is to collect and store the data in a database form to manage and maintain them from the perspective of human reliability analysis. All information on the human errors taken by operators in the power plant should be systematically collected and documented in its management. KAERI is developing the simulator-based HRA data handbook. In this study, the information required to store and manage the data necessary to perform an HRA as to store the HRA data to be stored in the handbook is identified and summarized. Especially this study is to summarize the collection and classification of qualitative data as the raw data to organize the data required to draw the HEP and its DB process. Qualitative HRA DB is a storehouse of all sub-information needed to receive the human error probability for Pasa. In this study, the requirements for structural design and implementation of qualitative HRA DB must be implemented for HRA DB were summarized. The follow-up study of the quantitative HRA DB implementation should be followed to draw the substantial HEP

  14. Applications of an improved quantitative in vitro assay for cell migration/invasion

    International Nuclear Information System (INIS)

    A qualitative, in vitro assay of cell migration/invasion through basement membrane, a physiological barrier to normal and metastatic cells travelling to extravascular sites, has been developed. The method utilizes Indium-111-labeled cells and measures objectively (a) adherence to, (b) migration into and (c) migration through human amnion membrane. The assay was used successfully to measure human peripheral blood polymorphonuclear leukocytes (PMNLs) and alveolar macrophage migration. Addition of protease inhibitors to assays of PMNL migration provided information which suggested that the protease human leukocytic elastase, plays a role in PMNL migration through physicological membranes. No consistent evidence, however, was obtained to support a direct role for oxygen radicals. Migration of peripheral blood PMNLs and alveolar macrophages of smokers and non-smokers was compared. No difference was observed. The relative invasiveness of two clones and two sublines of a murine fibrosarcoma cell line was also evaluated by the amnion assay. Differences were minimal and the reliability of the assay with the tumor cells is still uncertain. The relative invasion rates of the fibrosarcoma cells into the amnion membrane, however, were compared to the following in vivo properties of the same clones and sublines, with several other clones: (a) invasion rates of subcutaneous tumors into adjacent muscle, (b) collagenolytic activities in tumor homogenates, (c) metastatic potential, and (d) tumor growth rate. No definite conclusions could be drawn. Significant correlations between the collagenolytic activities and invasiveness supported a need for further investigation in this area

  15. Radioactive wastes assay technique and equipment

    International Nuclear Information System (INIS)

    The waste inventory records such as the activities and radio- nuclides contained in the waste packages are to be submitted with the radioactive wastes packages for the final disposal. The nearly around 10,000 drums of waste stocked in KAERI now should be assayed for the preparation of the waste inventory records too. For the successive execution of the waste assay, the investigation into the present waste assay techniques and equipment are to be taken first. Also the installation of the waste assay equipment through the comprehensive design, manufacturing and procurement should be proceeded timely. As the characteristics of the KAERI-stocked wastes are very different from that of the nuclear power plant and those have no regular waste streams, the application of the in-direct waste assay method using the scaling factors are not effective for the KAERI-generated wastes. Considering for the versal conveniency including the accuracy over the wide range of waste forms and the combination of assay time and sensitivity, the TGS(Tomographic Gamma Scanner) is appropriate as for the KAERI -generated radioactive waste assay equipment

  16. Mobile non-destructive assay system

    International Nuclear Information System (INIS)

    A mobile system for non-destructive assay (NDA), developed at the Los Alamos National Laboratory, provides accurate and sensitive measurements for transuranic (TRU) isotopes contained in 208-iota drums of miscellaneous nuclear wastes. The NDA unit consists of four major subsystems: an assay chamber, counting and digital electronics, data acquisition, and a neutron generator. It performs both active and passive neutron waste measurements. The former determines the amount of fissile isotopes at a sensitivity level of 1 mg plutonium. The latter determines spontaneous fission and α,n) isotopes at a comparable level. A complete assay consists of sequential active and passive measurements. The assay measurement and other supporting data are incorporated in a commercial spreadsheet program (Lotus 1,2,3) for further analysis, which includes various matrix corrections and a determination of whether or not the drum exceeds the 100-nCi/g threshold for TRU wastes. Field tests have been performed on three separate occasions, accomplishing more than 1800 waste drum assays. These waste drum assays are discussed, especially those comparing passive and active neutron measurements with independent segmented gamma scan assays. Results obtained with a set of 15 drums containing plutonium prepared from standards and actual hot waste matrices are also reviewed

  17. Simple UV spectrophotometric assay of Mefenamic acid

    Directory of Open Access Journals (Sweden)

    Dr. Safila Naveed

    2014-07-01

    Full Text Available Mefenamic acid belongs to non-steroidal antiinflammatory drugs (NSAID.. It is being used widely for the treatment of analgesia. It is also used as antirheumatic and antipyretic drug. Our aim of study is to develop a efficient least time consuming and simple spectrophotometric method for the assay of mefenamic acid. Comparision of assay of three different brands of mefenamic acid (mefnac,ponstan,dolar available in public medical store of Karachi, Pakistan has also been done. The assay is based on the ultraviolet UV absorbance maxima at about 288nm wavelength of mefenamic acid, water is used as solvent. A sample of drug was dissolved in water to produce a solution containing mefenamic acid. Similarly, a sample of ground tablets of different brand were dissolved in water and various dilutions were made. The absorbance of sample preparation was measured at 288nm against the solvent blank and the assay was determined by comparing with the absorbance of available brand. Our results reveals that among all the three brands of mefenamic acid (mefnac,ponstan,dolar rosulin and rovista shows highest percentage assay 107.5%. xplended and rosubar shows percent assay of 106.25% and 103.75% while rovactor shows lowest value for percentage assay 98.75%.

  18. A smartphone-readable barcode assay for the detection and quantitation of pesticide residues.

    Science.gov (United States)

    Guo, Juan; Wong, Jessica X H; Cui, Caie; Li, Xiaochun; Yu, Hua-Zhong

    2015-08-21

    In this paper, we present a smartphone-readable barcode assay for the qualitative detection of methyl parathion residues, a toxic organophosphorus pesticide that is popularly used in agriculture worldwide. The detection principle is based on the irreversible inhibition of the enzymatic activity of acetylcholinesterase (AchE) by methyl parathion; AchE catalytically hydrolyzes acetylthiocholine iodine to thiocholine that in turn dissociates dithiobis-nitrobenzoate to produce a yellow product (deprotonated thio-nitrobenzoate). The yellow intensity of the product was confirmed to be inversely dependent on the concentration of the pesticide. We have designed a barcode-formatted assay chip by using a PDMS (polydimethylsiloxane) channel plate (as the reaction reservoir), situated under a printed partial barcode, to complete the whole barcode such that it can be directly read by a barcode scanning app installed on a smartphone. The app is able to qualitatively present the result of the pesticide test; the absence or a low concentration of methyl parathion results in the barcode reading as "-", identifying the test as negative for pesticides. Upon obtaining a positive result (the app reads a "+" character), the captured image can be further analyzed to quantitate the methyl parathion concentration in the sample. Besides the portability and simplicity, this mobile-app based colorimetric barcode assay compares favorably with the standard spectrophotometric method. PMID:26087169

  19. Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study

    International Nuclear Information System (INIS)

    For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay, based on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by living cells was modified from a single-point towards a proliferation assay. This assay can be performed with a large number of samples in short time using multi-well-plates, assays can be performed semi-automatically with a microplate reader. Survival, the calculated parameter in this assay, is determined mathematically. Exponential growth in both control and irradiated groups was proven as the underlying basis of the applicability of the multiple MTT assay. The equivalence to a clonogenic survival assay with its disadvantages such as time consumption was proven in two setups including plating of cells before and after irradiation. Three cell lines (A 549, LN 229 and F 98) were included in the experiment to study its principal and general applicability

  20. Environmental herbicides and mycotoxin by inmunoraciochemical assay

    International Nuclear Information System (INIS)

    Immunochemical assays based on antigen antibody recognition, are at present very attractive analytical tools for determination of molecules present in different matrixes.Due to its specificity, sensitivity and easy application, the Immuno radiochemical assays have been adopted by international agencies for control procedures of environmental impact analytes. Optimization conditions for two contaminants, aflatoxin-B1 (AfB1, bacterial myco toxin from Aspergillus flavus) in dry food and atrazine (Atr, chloro-derivative triazine herbicides) in milk and water, by immunoradiometric assays based on the use of polyclonal antibodies for the mycotoxins and specific monoclonal antibody for the triazine derivatives, are presented.Liquid Chromatography is used as reference

  1. Radioreceptor assay of human growth hormone

    International Nuclear Information System (INIS)

    A radioreceptor assay for human growth hormone (hGH) was developed. The receptor preparation was 25,000g pellet from the livers of pregnant rabbits. Iodination of GH with 125I was preformed by the methods of Lactoperoxidase and Iodogen. The sensitivity of assay was 0.67 ± 0.11 ng/ml serum. Serum hGH levels in 72 cases of normal subjects, 102 cases of acromegaly were measured by radioreceptor assay (RRA), and the results were compared with those obtained by radioimmunoassay (RIA)

  2. Passive neutron assay of irradiated nuclear fuels

    International Nuclear Information System (INIS)

    Passive neutron assay of irradiated nuclear fuel has been investigated by calculations and experiments as a simple, complementary technique to the gamma assay. From the calculations it is found that the neutron emission arises mainly from the curium isotopes, the neutrons exhibit very good penetrability of the assemblies, and the neutron multiplication is not affected by the burnup. From the experiments on BWR and PWR assemblies, it is found that the neutron emission rate is proportional to burnup raised to 3.4 power. Recent investigations indicate that the passive neutron assay is a simple and useful technique to determine the consistency of burnups between assemblies. 10 refs

  3. Passive neutron assay of irradiated nuclear fuels

    International Nuclear Information System (INIS)

    Passive neutron assay of irradiated nuclear fuel has been investigated by calculations and experiments as a simple, complementary technique to the gamma assay. From the calculations it was found that the neutron emission arises mainly from the curium isotopes, the neutrons exhibit very good penetrability of the assemblies, and the neutron multiplication is not affected by the burnup. From the experiments on BWR and PWR assemblies, the neutron emission rate is proportional to burnup raised to 3.4 power. The investigations indicate that the passive neutron assay is a simple and useful technique to determine the consistency of burnups between assemblies

  4. Nondestructive assay measurements applied to reprocessing plants

    International Nuclear Information System (INIS)

    Nondestructive assay for reprocessing plants relies on passive gamma-ray spectrometry for plutonium isotopic and plutonium mass values of medium-to-low-density samples and holdup deposits; on active x-ray fluorescence and densitometry techniques for uranium and plutonium concentrations in solutions; on calorimetry for plutonium mass in product; and passive neutron techniques for plutonium mass in spent fuel, product, and waste. This paper will describe the radiation-based nondestructive assay techniques used to perform materials accounting measurements. The paper will also discuss nondestructive assay measurements used in inspections of reprocessing plants

  5. Qualitative Research and Computer Analysis: New Challenges and Opportunities

    OpenAIRE

    Yuen, AHK

    2000-01-01

    The use of computers for Qualitative Data Analysis (QDA) in qualitative research has been growing rapidly in the last decade. QDA programs are software packages developed explicitly for the purpose of analyzing qualitative data. A range of different kinds of program is available for the handling and analysis of qualitative data, such as Atlas/ti, HyperRESEARCH, and NUD*IST. With the development of new technologies, the QDA software has advanced from the efficient code-and-retrieve ability to ...

  6. PRIORITY RESEARCH DIRECTIONS IN THE AREA OF QUALITATIVE METHODOLOGY

    OpenAIRE

    Melnikova, Olga; Khoroshilov, Dmitry

    2010-01-01

    The basic directions of modern theoretical and practical research in the area of qualitative methodology in Russia are discussed in the article. The complexity of research is considered from three points of view: the development of methodology of qualitative analysis, qualitative methods, and verbal and nonverbal projective techniques. The authors present an integrative model of the qualitative analysis, the research on specificity of the use of discourse-analysis method and projective techni...

  7. Travelling Methods: Tracing the Globalization of Qualitative Communication Research

    OpenAIRE

    Bryan C. TAYLOR; Thomas R. LINDLOF

    2013-01-01

    Existing discussion of the relationships between globalization, communication research, and qualitative methods emphasizes two images: the challenges posed by globalization to existing communication theory and research methods, and the impact of post-colonial politics and ethics on qualitative research. We draw in this paper on a third image – qualitative research methods as artifacts of globalization – to explore the globalization of qualitative communication research methods. Following a...

  8. Live-cell luciferase assay of drug resistant cells

    OpenAIRE

    sprotocols

    2015-01-01

    To date, multiplexing cell-based assay is essential for high-throughput screening of molecular targets. Measuring multiple parameters of a single sample increases consistency and decrease time and cost of assay. Functional assay of living cell is useful as a first step of multiplexing assay, because live-cell assay allows following second assay using cell lysate or stained cell. However, live-cell assay of drug resistant cells that are highly activated of drug efflux mechanisms is sometimes u...

  9. Critical Issues in the Funding of Qualitative Research

    Science.gov (United States)

    Bourgeault, Ivy Lynn

    2012-01-01

    Qualitative research has moved from the margins to the mainstream in many domains of scholarship. Yet, biases against how qualitative methods can best address important research questions still persist. The present article provides reflections regarding my experiences of proposing and reviewing both qualitative and quantitative research grants for…

  10. Qualitative Research and Its Place in Psychological Science

    Science.gov (United States)

    Madill, Anna; Gough, Brendan

    2008-01-01

    In discussing the place of diverse qualitative research within psychological science, the authors highlight the potential permeability of the quantitative-qualitative boundary and identify different ways of increasing communication between researchers specializing in different methods. Explicating diversity within qualitative research is…

  11. Origins, Methods and Advances in Qualitative Meta-Synthesis

    Science.gov (United States)

    Nye, Elizabeth; Melendez-Torres, G. J.; Bonell, Chris

    2016-01-01

    Qualitative research is a broad term encompassing many methods. Critiques of the field of qualitative research argue that while individual studies provide rich descriptions and insights, the absence of connections drawn between studies limits their usefulness. In response, qualitative meta-synthesis serves as a design to interpret and synthesise…

  12. Extending Engineering Practice Research with Shared Qualitative Data

    Science.gov (United States)

    Trevelyan, James

    2016-01-01

    Research on engineering practice is scarce and sharing of qualitative research data can reduce the effort required for an aspiring researcher to obtain enough data from engineering workplaces to draw generalizable conclusions, both qualitative and quantitative. This paper describes how a large shareable qualitative data set on engineering…

  13. Research Commentary: The Promise of Qualitative Metasynthesis for Mathematics Education

    Science.gov (United States)

    Thunder, Kateri; Berry, Robert Q., III.

    2016-01-01

    Mathematics education has benefited from qualitative methodological approaches over the past 40 years across diverse topics. Although the number, type, and quality of qualitative research studies in mathematics education has changed, little is known about how a collective body of qualitative research findings contributes to our understanding of a…

  14. How Is Qualitative Research Taught at the Masters' Level?

    Science.gov (United States)

    Drisko, James W.

    2008-01-01

    This study examined how qualitative research is taught in foundation MSW courses using a content analysis of syllabi and a survey. The Council on Social Work Education required qualitative research content in 1994 and several authors advocate for greater inclusion of it. Yet no research about what qualitative content is included on syllabi is…

  15. Application of qualitative research in management (why, when and how)

    OpenAIRE

    Gholamreza JandaghI; Hasan Zarei Matin

    2010-01-01

    Qualitative research has been increasingly used in all sciences. In this paper we will discussabout the applications of qualitative research methods in management sciences. The differencesbetween quantitative and qualitative researches will be clarified and the statistical methods whichare suitable for such researches will be explored.

  16. QUALITATIVE RESEARCH IN AGRICULTURAL ECONOMICS: PARADIGMS, PURPOSES, AND EVALUATION CRITERIA

    OpenAIRE

    Bitsch, Vera

    2001-01-01

    The qualitative paradigm is not widely discussed in agricultural economics, although research strategies are applied. The purpose of this contribution is (1) to elaborate on the paradigm of qualitative research; (2) to introduce purposes of qualitative research and provide examples; and (3) to discuss criteria of scientific rigor applicable.

  17. An Exemplar for Teaching and Learning Qualitative Research

    Science.gov (United States)

    Onwuegbuzie, Anthony J.; Leech, Nancy L.; Slate, John R.; Stark, Marcella; Sharma, Bipin; Frels, Rebecca; Harris, Kristin; Combs, Julie P.

    2012-01-01

    In this article, we outline a course wherein the instructors teach students how to conduct rigorous qualitative research. We discuss the four major distinct, but overlapping, phases of the course: conceptual/theoretical, technical, applied, and emergent scholar. Students write several qualitative reports, called qualitative notebooks, which…

  18. Conducting Qualitative Research: A Practical Guide for School Counselors

    Science.gov (United States)

    Farber, Nancy K.

    2006-01-01

    This article discusses the concept of school counselor as researcher. Qualitative research is defined, explained, and differentiated from quantitative research. School counselor questions that lend themselves to qualitative research are explored. The article also discusses the steps of qualitative research in depth, including developing questions,…

  19. Strategically Reviewing the Research Literature in Qualitative Research

    Science.gov (United States)

    Chenail, Ronald J.; Cooper, Robin; Desir, Charlene

    2010-01-01

    Reviewing literature in qualitative research can be challenging in terms of why, when, where, and how we should access third-party sources in our work, especially for novice qualitative researchers. As a pragmatic solution, we suggest qualitative researchers utilize research literature in four functional ways: (a) define the phenomenon in…

  20. Stochastic modeling for the COMET-assay

    OpenAIRE

    Boulesteix, Anne-Laure; Hösel, Volker; Liebscher, Volkmar

    2003-01-01

    We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data analysis.

  1. ORNL TRU-waste-assay system

    International Nuclear Information System (INIS)

    ORNL has been selected as the demonstration site for a new transuranic neutron assay system developed at the Los Alamos National Laboratory. The major objectives of the cooperative interlaboratory program is to field test, calibrate, and evaluate the neutron interrogation system, to provide a demonstration and training facility for DOE of this sophisticated technology, to reduce the volume of TRU waste stored at ORNL, and to provide positive identification of the radionuclide content of the ORNL TRU waste. In order to meet these objectives, a two-tier assay system utilizing the LANL neutron interrogation assay system and an upgraded gamma-ray drum scanner has been employed. Each assay technique and the results obtained from the examination of authentic ORNL TRU waste are described

  2. Proximity assays for sensitive quantification of proteins

    Directory of Open Access Journals (Sweden)

    Christina Greenwood

    2015-06-01

    Full Text Available Proximity assays are immunohistochemical tools that utilise two or more DNA-tagged aptamers or antibodies binding in close proximity to the same protein or protein complex. Amplification by PCR or isothermal methods and hybridisation of a labelled probe to its DNA target generates a signal that enables sensitive and robust detection of proteins, protein modifications or protein–protein interactions. Assays can be carried out in homogeneous or solid phase formats and in situ assays can visualise single protein molecules or complexes with high spatial accuracy. These properties highlight the potential of proximity assays in research, diagnostic, pharmacological and many other applications that require sensitive, specific and accurate assessments of protein expression.

  3. Safeguards and Non-destructive Assay

    Energy Technology Data Exchange (ETDEWEB)

    Carchon, R.; Bruggeman, M

    2001-04-01

    SCK-CEN's programme on safeguards and non-destructive assay includes: (1) various activities to assure nuclear materials accountancy; (2) contributes to the implementation of Integrated Safeguards measures in Belgium and to assist the IAEA through the Belgian Support Programme; (3) renders services to internal and external customers in the field of safeguards; (4) improves passive neutron coincidence counting techniques for waste assay and safeguards verification measurements by R and D on correlation algorithms implemented via software or dedicated hardware; (5) improves gamma assay techniques for waste assay by implementing advanced scanning techniques and different correlation algorithms; and (6) develops numerical calibration techniques. Major achievements in these areas in 2000 are reported.

  4. A novel parameter in comet assay measurements

    OpenAIRE

    Kirilova Milena; Ivanov Rumen; Miloshev George

    2005-01-01

    Single Cell Gel Electrophoresis (SCGE) or Comet assay is a very sensitive method for assessing damages in DNA on a single cell level. It has found many applications in fields where genotoxic activity could be an issue. In environmental monitoring, health care, food industry Comet assay is used with increasing popularity. For verifying the results obtained by this method many parameters could be monitored. To that end several software packages exist. In the conditions that we are suggesting on...

  5. The comet assay in nanotoxicology research.

    OpenAIRE

    Karlsson, Hanna L

    2010-01-01

    Nanoscale particles can have impressive and useful characteristics, but the same properties may be problematic for human health. From this perspective it is critical to assess the ability of nanoparticles to cause DNA damage. This review focuses on the use of the comet assay in nanotoxicology research. In the alkaline version of the assay, DNA strand breaks and alkali-labile sites are detected and oxidatively damaged DNA can be analyzed using the enzyme formamidopyrimidine glycosylase. The ar...

  6. Automated optical sensing system for biochemical assays

    Science.gov (United States)

    Oroszlan, Peter; Duveneck, Gert L.; Ehrat, Markus; Widmer, H. M.

    1994-03-01

    In this paper, we present a new system called FOBIA that was developed and optimized with respect to automated operation of repetitive assay cycles with regenerable bioaffinity sensors. The reliability and precision of the new system is demonstrated by an application in a competitive assay for the detection of the triazine herbicide Atrazine. Using one sensor in more than 300 repetitive cycles, a signal precision better than 5% was achieved.

  7. Passive nondestructive assay of nuclear materials

    International Nuclear Information System (INIS)

    The term nondestructive assay (NDA) is applied to a series of measurement techniques for nuclear fuel materials. The techniques measure radiation induced or emitted spontaneously from the nuclear material; the measurements are nondestructive in that they do not alter the physical or chemical state of the nuclear material. NDA techniques are characterized as passive or active depending on whether they measure radiation from the spontaneous decay of the nuclear material or radiation induced by an external source. This book emphasizes passive NDA techniques, although certain active techniques like gamma-ray absorption densitometry and x-ray fluorescence are discussed here because of their intimate relation to passive assay techniques. The principal NDA techniques are classified as gamma-ray assay, neutron assay, and calorimetry. Gamma-ray assay techniques are treated in Chapters 1--10. Neutron assay techniques are the subject of Chapters 11--17. Chapters 11--13 cover the origin of neutrons, neutron interactions, and neutron detectors. Chapters 14--17 cover the theory and applications of total and coincidence neutron counting. Chapter 18 deals with the assay of irradiated nuclear fuel, which uses both gamma-ray and neutron assay techniques. Chapter 19 covers perimeter monitoring, which uses gamma-ray and neutron detectors of high sensitivity to check that no unauthorized nuclear material crosses a facility boundary. The subject of Chapter 20 is attribute and semiquantitative measurements. The goal of these measurements is a rapid verification of the contents of nuclear material containers to assist physical inventory verifications. Waste and holdup measurements are also treated in this chapter. Chapters 21 and 22 cover calorimetry theory and application, and Chapter 23 is a brief application guide to illustrate which techniques can be used to solve certain measurement problems

  8. Qualität und Qualitätsmanagement in der universitären naturwissenschaftlichen Lehrerfortbildung

    OpenAIRE

    Ansorge-Grein, Kerstin

    2011-01-01

    Vor dem Hintergrund der politischen Entwicklungen in Hessen und der allgemeinen Debatte über Qualität im Bildungsbereich sollte im Lehrerfortbildungszentrum Chemie (lfbz-Chemie) der Universität Frankfurt am Main eine systematische Qualitätsentwicklung etabliert und dieses Vorhaben wissenschatflich begleitet werden. Die wissenschaftliche Arbeit besteht aus drei Säulen: - Eine erste, qualitative Studie sollte Qualitätskriterien und -indikatoren liefern. - In einer Fallstudie am lfbz-Chemie Fran...

  9. Un quaderno di qualitá

    Directory of Open Access Journals (Sweden)

    M.E. Damiani

    2003-05-01

    Full Text Available

    Obiettivo: sviluppare un sistema qualità per il Dipartimento di Sanità Pubblica, coerente con la politica aziendale, l’accreditamento regionale e le ISO Vision 2000, in cui il cuore è dato dal governo clinico, perseguendo obiettivi di salute e coniugando l’efficacia del sistema all’efficienza.

    Materiali e Metodi: attività di formazione intervento strutturata in corsi formativi ed incontri periodici indirizzati alla Direzione del Dipartimento, ai referenti Qualità individuati in ogni Servizio, oltre ad incontri di sensibilizzazione sul percorso con gli altri operatori del DSP.

    Risultati: sono stati definiti l’offerta di prodotti/servizi e dei relativi fattori qualitativi, i processi operativi (attività, responsabilità, strumenti e interfacce, il coinvolgimento della direzione (la politica e l’adesione al percorso, i sistemi di comunicazione (esterna ed interna, il sistema informativo per la verifica dei risultati, le modalità di realizzazione delle verifiche interne e del sistema informativo come supporto alle decisioni (il sistema informativo, la programmazione annuale ed il processo di budget. La realizzazione del testo “Quaderno di appunti sulla Qualità in Sanità” ha ulteriormente contribuito a diffondere agli operatori i fondamenti teorici del Sistema Qualità sviluppato, oltre alle tecniche e agli strumenti acquisiti.

    Conclusioni: il percorso ha portato a presidiare gli aspetti fondamentali del sistema organizzativo e a documentarne l’attività, ma anche ad individuare nuove criticità e opportunità di sviluppo: come la progettazione e l’utilizzo di sistemi informativiinformatici per la rilevazione dei dati e per la restituzione di informazioni gestionali, la necessità di un confronto maggiormente strutturato con l’utenza, di una verifica più approfondita dei risultati, mediante il riesame della

  10. The qualitative criterion of transient angle stability

    DEFF Research Database (Denmark)

    Lyu, R.; Xue, Y.; Xue, F.;

    2015-01-01

    In almost all the literatures, the qualitative assessment of transient angle stability extracts the angle information of generators based on the swing curve. As the angle (or angle difference) of concern and the threshold value rely strongly on the engineering experience, the validity and robust of...... these criterions are weak. Based on the stability mechanism from the extended equal area criterion (EEAC) theory and combining with abundant simulations of real system, this paper analyzes the criterions in most literatures and finds that the results could be too conservative or too optimistic. It is...

  11. How public perceive diabetes: A qualitative study

    OpenAIRE

    Abdoli, Samereh; Mardanian, Leila; Mirzaei, Marjan

    2012-01-01

    Background: Diabetes has a high prevalence in Iran, and its incidence is estimated to increase from 3.5 million adults in 2005 to 5.1 million by 2025. Given the high prevalence of diabetes in Iranians, it is surprising that little is known about understanding of diabetes in the general population. This study aimed to explore how people without diabetes interpreted the disease. Materials and Methods: This study was conducted as a qualitative content analysis, using unstructured and in-depth in...

  12. Qualitative evolution in f(R) cosmologies

    CERN Document Server

    Avagyan, R M; Harutyunyan, G H; Saharian, A A

    2016-01-01

    We investigate the qualitative evolution of (D+1)-dimensional cosmological models in f(R) gravity for the general case of the function f(R). The analysis is specified for various examples, including the (D+1)-dimensional generalization of the Starobinsky model, models with polynomial and exponential functions. The cosmological dynamics are compared in the Einstein and Jordan representations of the corresponding scalar-tensor theory. The features of the cosmological evolution are discussed for Einstein frame potentials taking negative values in certain regions of the field space.

  13. Comparative qualitative research in Cultural Psychology

    DEFF Research Database (Denmark)

    Demuth, Carolin; Fatigante, Marilena

    2012-01-01

    The present paper aims to provide an approach that allows to study the interplay of culture and psychological human functioning in comparative study designs. Starting out with a brief overview of how qualitative, cultural, and comparative research is addressed in the field of psychology we...... will take a Cultural Psychology approach to suggest that the unit of analysis for comparative research needs to be situated social interaction. We will then suggest an integrative approach that allows us to study social interaction both on a micro- and on a macro-level by combining discourse analysis...... some criteria of validity that particularly apply to the field of comparative research in Cultural Psychology....

  14. Analysis of uncertainties in isotopic assay of UO2 pellets using thermal ionization mass spectrometer

    International Nuclear Information System (INIS)

    Control Laboratory being a centralized analytical facility of NFC is responsible for isotopic assay of UO2 pellets by Thermal ionization mass spectrometry (TIMS). TIMS is a precise and accurate technique for the determination of isotopic ratios. The present work involves the evaluation of uncertainty components affecting the measurement results. Qualitative and Quantitative Uncertainty factors have been evaluated. Qualitative factors mentioned are mostly related to technical competency of operators involved in the analysis; while quantitative factors have been evaluated. The isotope reference material selected as check standard is SRM U-005 Uranium Isotopic Standard with 235U (at%): 0.4833 ± 0.0005 and the Natural isotopic composition of 235U sample has been selected for analysis to calculate uncertainty sources effecting the obtained isotopic ratios

  15. Radioimmune assay of human platelet prostaglandin synthetase

    International Nuclear Information System (INIS)

    Normal platelet function depends, in part, on platelet PG synthesis. PG synthetase (cyclo-oxygenase) catalyzes the first step in PG synthesis, the formation of PGH2 from arachidonic acid. Inhibition of the enzyme by ASA results in an abnormality in the platelet release reaction. Patients with pparent congenital abnormalities in the enzyme have been described, and the effects have been referred to as ''aspirin-like'' defects of the platelet function. These patients lack platelet PG synthetase activity, but the actual content of PG synthetase protein in these individuals' platelets is unknown. Therefore an RIA for human platelet PG synthetase would provide new information, useful in assessing the aspirin-like defects of platelet function. An RIA for human platelet PG synthetase is described. The assay utilizes a rabbit antibody directed against the enzyme and [125I]-labelled sheep PG synthetase as antigen. The human platelet enzyme is assayed by its ability to inhibit precipitation of the [125I]antigen. The assay is sensitive to 1 ng of enzyme. By the immune assay, human platelets contain approximately 1200 ng of PG synethetase protein per 1.5 mg of platelet protein (approximately 109 platelets). This content corresponds to 10,000 enzyme molecules per platelet. The assay provides a rapid and convenient assay for the human platelet enzyme, and it can be applied to the assessment of patients with apparent platelet PG synthetase (cyclo-oxygenase) deficiency

  16. An ultrafiltration assay for lysyl oxidase.

    Science.gov (United States)

    Shackleton, D R; Hulmes, D J

    1990-03-01

    A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Enzyme activity is determined by scintillation counting of the ultrafiltrate, after subtraction of radioactivity released in the presence of beta-aminopropionitrile, a specific inhibitor of the enzyme. Conditions are described which optimize both the sensitivity and the efficient use of substrate. The assay shows linear inhibition of activity in up to 1 M urea; hence, as the enzyme is normally diluted in the assay, samples in 6 M urea can be assayed directly, without prior dialysis, and corrected for partial inhibition. Comparable results are obtained when enzyme activity is assayed by ultrafiltration or microdistillation. The assay is simple and convenient and, by using disposable containers throughout, it eliminates the need for time-consuming decontamination of radioactive glassware. PMID:1971160

  17. Hepatitis C virus: Screening, diagnosis, and interpretation of laboratory assays

    Directory of Open Access Journals (Sweden)

    Ekta Gupta

    2014-01-01

    Full Text Available An estimated 3% of the world population is infected with Hepatitis C virus (HCV, a hepatotropic RNA virus, transmitted primarily via the blood route. The major modes of transmission of the virus include injection drug use, unsafe injection practices, blood transfusion etc. HCV causes chronic hepatitis in about 80% of those infected by it. The mainstay in diagnosing infection with HCV is to initially screen high risk groups for antibodies to HCV (anti-HCV. The inclusion of serum to cut-off ratio (S/CO in recent guidelines is helpful in deciding the supplemental assay to be used to confirm initially reactive screening results. Nucleic acid amplification tests (NAT are used as confirmatory tools, and also to determine viral load prior to initiating treatment. Quantitative NAT has replaced qualitative assays. Genotyping is an important tool in clinical management to predict the likelihood of response and determine the optimal duration of therapy. The impact of this infection has begun to emerge in India. The problem of professional blood donation despite an existing law against it, and flourishing unsafe injection practices, are potential sources for the spread of hepatitis C in our country. All health care practitioners need to understand how to establish or exclude a diagnosis of HCV infection and to interpret the tests correctly. In the absence of a preventive or therapeutic vaccine, and also of post-exposure prophylaxis against the virus, it is imperative to diagnose infection by HCV so as to prevent hepatic insult and the ensuing complications that follow, including primary hepatocellular carcinoma (HCC. This review aims to help blood bank staff regarding options for diagnosis and management of donors positive for HCV.

  18. Tagungsbericht: Sixth Annual Meeting of Qualitative Psychology "Generalization in Qualitative Psychology"

    OpenAIRE

    Gürtler, Leo; Gahleitner, Silke-Birgitta

    2006-01-01

    Der Konferenzbericht gibt einen Überblick über die sechste Tagung der Initiative Qualitative Psychologie, die vom 21.-23. Oktober 2005 in Velden, Österreich, vom Zentrum für Qualitative Psychologie (Tübingen) organisiert wurde. Die Tagung hat bereits eine mehrjährige Tradition und wurde wie gewohnt von Forscher(inne)n unterschiedlichster Professionen aus verschieden Ländern besucht. Der Schwerpunkt der diesjährigen Konferenz widmete sich der Frage, wie der Aspekt der Generalisierung in der qu...

  19. Conference Report: Fourth Annual Meeting of Qualitative Psychology. "Areas of Qualitative Psychology - Special Focus on Design"

    OpenAIRE

    Gürtler, Leo; Gahleitner, Silke-Birgitta

    2004-01-01

    This report note reviews the fourth an­nual meeting of Qualitative Psychology in Blau­beu­ren (near Ulm, Germany) Oct., 22-24, 2003. Organized by the Center for Qualitative Psy­chol­ogy (Tübingen, Germany). The question of Re­search Design was chosen as the central topic of the conference. Researchers from different pro­fes­sions took part. The range of experience of the participants was very heterogeneous: Beginning with young researchers, different levels of exper­tise were represented (up ...

  20. Development and validation of a Pneumocystis jirovecii real-time polymerase chain reaction assay for diagnosis of Pneumocystis pneumonia

    OpenAIRE

    Church, Deirdre L; Ambasta, Anshula; Wilmer, Amanda; Williscroft, Holly; Ritchie, Gordon; Pillai, Dylan R.; Champagne, Sylvie; Daniel G Gregson

    2015-01-01

    Pneumocystis pneumonia is caused by Pneumocystis jirovecii, an opportunistic fungal pathogen. Presently, many clinical microbiology laboratories rely on direct microscopic detection of P jirovecii. The validation, and clinical and laboratory development of a qualitative P jirovecii real-time polymerase chain reaction assay for the rapid detection of Pneumocystis pneumonia is discussed by the authors. In addition, this new technique is compared with the existing gold-standard immunofluorescenc...

  1. Qualitative Methodology in Analyzing Educational Phenomena

    Directory of Open Access Journals (Sweden)

    Antonio SANDU

    2010-12-01

    Full Text Available Semiological analysis of educational phenomena allow researchers access to a multidimensional universe of meanings that is represented by the school, not so much seen as an institution, but as a vector of social action through educational strategies. We consider education as a multidimensional phenomenon since its analysis allows the researcher to explore a variety of research hypotheses of different paradigmatic perspectives that converge in an educational finality. According to the author Simona Branc one of the most appropriate methods used in qualitative data analysis is Grounded Theory; this one assumes a systematic process of generating concepts and theories based on the data collected. Specialised literature defines Grounded Theory as an inductive approach that starts with general observations and during the analytical process creates conceptual categories that explain the theme explored. Research insist on the role of the sociologic theory of managing the research data and for providing ways of conceptualizing the descriptions and explanations.Qualitative content analysis is based on the constructivist paradigm (constructionist in the restricted sense that we used previously. It aims to create an “understanding of the latent meanings of the analyzed messages”. Quantitative content analysis involves a process of encoding and statistical analysis of data extracted from the content of the paper in the form of extractions like: frequencies, contingency analysis, etc

  2. Qualitative simulation in formal process modelling

    International Nuclear Information System (INIS)

    In relation to several different research activities at the OECD Halden Reactor Project, the usefulness of formal process models has been identified. Being represented in some appropriate representation language, the purpose of these models is to model process plants and plant automatics in a unified way to allow verification and computer aided design of control strategies. The present report discusses qualitative simulation and the tool QSIM as one approach to formal process models. In particular, the report aims at investigating how recent improvements of the tool facilitate the use of the approach in areas like process system analysis, procedure verification, and control software safety analysis. An important long term goal is to provide a basis for using qualitative reasoning in combination with other techniques to facilitate the treatment of embedded programmable systems in Probabilistic Safety Analysis (PSA). This is motivated from the potential of such a combination in safety analysis based on models comprising both software, hardware, and operator. It is anticipated that the research results from this activity will benefit V and V in a wide variety of applications where formal process models can be utilized. Examples are operator procedures, intelligent decision support systems, and common model repositories (author) (ml)

  3. [Reflexivity as the ethos of qualitative research].

    Science.gov (United States)

    Minayo, Maria Cecília de Souza; Guerriero, Iara Coelho Zito

    2014-04-01

    This paper seeks to promote reflection on ethics in anthropological and qualitative research and emphasize the comprehensive, relational and reflective character of this process, as well as the advantages and problems that arise from different logic and often conflicting interests between researchers and their interlocutors. The text is divided into four parts and addresses the ethical: (a) significance of these approaches; (b) behavior of the researcher in the field; (c) analysis of the empirical material; and (d) considerations in the preparation of results of anthropological and qualitative studies, using some classic examples from the international literature. The paper concludes by reflecting on the distinction between the requirements of the Ethics Committee and the Ethics of research itself. It must be clear that the comprehensive sense of ethics which includes the responsibility of the researcher cannot be condensed in the instruments required for the judgment of projects because the following elements are involved in the development of research, namely the social significance of the work, the institutional relations with fund providers, how to treat staff and research students in academic work and commitments with the scientific community. PMID:24820593

  4. Environmental Risk Communication through Qualitative Risk Assessment

    Directory of Open Access Journals (Sweden)

    Sabre J. Coleman

    2014-06-01

    Full Text Available Environmental analysts are often hampered in communicating the risks of environmental contaminants due to the myriad of regulatory requirements that are applicable. The use of a qualitative, risk-based control banding strategy for assessment and control of potential environmental contaminants provides a standardized approach to improve risk communication. Presented is a model that provides an effective means for determining standardized responses and controls for common environmental issues based on the level of risk. The model is designed for integration within an occupational health and safety management system to provide a multidisciplinary environmental and occupational risk management approach. This environmental model, which utilizes multidisciplinary control banding strategies for delineating risk, complements the existing Risk Level Based Management System, a proven method in a highly regulated facility for occupational health and safety. A simplified environmental risk matrix is presented that is stratified over four risk levels. Examples of qualitative environmental control banding strategies are presented as they apply to United States regulations for construction, research activities, facility maintenance, and spill remediation that affect air, water, soil, and waste disposal. This approach offers a standardized risk communication language for multidisciplinary issues that will improve communications within and between environmental health and safety professionals, workers, and management.

  5. The Future of Qualitative Research in Psychology: Accentuating the Positive.

    Science.gov (United States)

    Gough, Brendan; Lyons, Antonia

    2016-06-01

    In this paper we reflect on current trends and anticipate future prospects regarding qualitative research in Psychology. We highlight various institutional and disciplinary obstacles to qualitative research diversity, complexity and quality. At the same time, we note some causes for optimism, including publication breakthroughs and vitality within the field. The paper is structured into three main sections which consider: 1) the positioning of qualitative research within Psychology; 2) celebrating the different kinds of knowledge produced by qualitative research; and 3) implementing high quality qualitative research. In general we accentuate the positive, recognising and illustrating innovative qualitative research practices which generate new insights and propel the field forward. We conclude by emphasising the importance of research training: for qualitative research to flourish within Psychology (and beyond), students and early career researchers require more sophisticated, in-depth instruction than is currently offered. PMID:26179872

  6. The Comet Assay: Tails of the (Unexpected. Use of the comet assay in pharmaceutical development.

    Directory of Open Access Journals (Sweden)

    Bas-jan Van Der Leede

    2015-08-01

    Full Text Available In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance. In this presentation we want to give insight into the circumstances in vivo comet assay is deployed in a Genetic Toxicology Department of a pharmaceutical company. As the in vivo comet assay is a salvage assay, it means that some events have occurred in an in vitro assay and that the compound (or metabolite responsible for this signal is potentially deselected for further development. More than often the decision to perform an in vivo comet assay is at a very early stage in development and the first time that the compound will be tested in vivo at high/toxic dose levels. As almost no toxicokinetic data and tissue distribution data are available a careful design with maximizes the chances for successful mitigation is necessary. Decisions on acute or repeated dosing need to be made and arrangements for combining the in vivo comet assay with the in vivo micronucleus assay are to be considered. Often synthesis methods need to be scaled up fast to provide the required amount of compound and information on suitable formulations needs to be in place. As exposure data is crucial for interpretation of results, analytical methods need to be brought in place rapidly. An experienced multi skilled and communicative team needs to be available to deploy successfully this kind of assays at an early stage of development. We will present a few scenarios on study conduct and demonstrate how this assay can make a difference for the further development of a new drug.

  7. Controlling variation in the comet assay

    Directory of Open Access Journals (Sweden)

    Andrew Richard Collins

    2014-10-01

    Full Text Available Variability of the comet assay is a serious issue, whether it occurs from experiment to experiment in the same laboratory, or between different laboratories analysing identical samples. Do we have to live with high variability, just because the comet assay is a biological assay rather than analytical chemistry? Numerous attempts have been made to limit variability by standardising the assay protocol, and the critical steps in the assay have been identified; agarose concentration, duration of alkaline incubation, and electrophoresis conditions (time, temperature and voltage gradient are particularly important. Even when these are controlled, variation seems to be inevitable. It is helpful to include in experiments reference standards, i.e. cells with a known amount of specific damage to the DNA. They can be aliquots frozen from a single large batch of cells, either untreated (negative controls or treated with, for example, H2O2 or X-rays to induce strand breaks (positive control for the basic assay, or photosensitiser plus light to oxidise guanine (positive control for Fpg- or OGG1-sensitive sites. Reference standards are especially valuable when performing a series of experiments over a long period - for example, analysing samples of white blood cells from a large human biomonitoring trial - to check that the assay is performing consistently, and to identify anomalous results necessitating a repeat experiment. The reference values of tail intensity can also be used to iron out small variations occurring from day to day. We present examples of the use of reference standards in human trials, both within one laboratory and between different laboratories, and describe procedures that can be used to control variation.

  8. Detection of Phaeocystis globosa using sandwich hybridization integrated with nuclease protection assay (NPA-SH)

    Institute of Scientific and Technical Information of China (English)

    ZHEN Yu; MI Tiezhu; YU Zhigang

    2008-01-01

    Phaeocystis globosa Scherffel is one of the common harmful algae species in coastal waters of the southeastern China. In this study, sandwich hybridization integrated with nuclease protection assay (NPA-SH) was used to qualitatively and quantitatively detect P. globosa. Results showed that this method had good applicability and validity in analyzing the samples from laboratory cultures and from fields. The linear regression equation for P. globosa was obtained, and the lowest detection number of cells was 1.8×104 cells. Statistics showed that there was no distinct difference between the results of detecting the microalgae by NPA-SH and traditional microscopy. This technique has good reliability, accuracy, and can give a remarkably high sample processing rate. Sandwich hybridization integrated with nuclease protection assay will provide an efficient alternative to microscopic method for monitoring and investigating the bloom of P. globosa.

  9. Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay.

    Science.gov (United States)

    Carroll, Karen C; Buchan, Blake W; Tan, Sokha; Stamper, Paul D; Riebe, Katherine M; Pancholi, Preeti; Kelly, Cheryl; Rao, Arundhati; Fader, Robert; Cavagnolo, Robert; Watson, Wendy; Goering, Richard V; Trevino, Ernest A; Weissfeld, Alice S; Ledeboer, Nathan A

    2013-12-01

    The Verigene Clostridium difficile Nucleic Acid test (Verigene CDF test) (Nanosphere, Northbrook, IL) is a multiplex qualitative PCR assay that utilizes a nanoparticle-based array hybridization method to detect C. difficile tcdA and tcdB in fecal specimens. In addition, the assay detects binary toxin gene sequences and the single base pair deletion at nucleotide 117 (Δ 117) in tcdC to provide a presumptive identification of the epidemic strain 027/NAP1/BI (referred to here as ribotype 027). This study compared the Verigene CDF test with anaerobic direct and enriched toxigenic culture on stool specimens from symptomatic patients among five geographically diverse laboratories within the United States. The Verigene CDF test was performed according to the manufacturer's instructions, and the reference methods performed by a central laboratory included direct culture onto cycloserine cefoxitin fructose agar (CCFA) and enriched culture using cycloserine cefoxitin mannitol broth with taurocholate and lysozyme. Recovered isolates were identified as C. difficile using gas liquid chromatography and were tested for toxin using a cell culture cytotoxicity neutralization assay. Strains belonging to ribotype 027 were determined by PCR ribotyping and bidirectional sequencing for Δ 117 in tcdC. A total of 1,875 specimens were evaluable. Of these, 275 specimens (14.7%) were culture positive by either direct or enriched culture methods. Compared to direct culture alone, the overall sensitivity, specificity, positive predictive value, and negative predictive value for the Verigene CDF test were 98.7%, 87.5%, 42%, and 99.9%, respectively. Compared to combined direct and enriched culture results, the sensitivity, specificity, positive predictive value, and negative predictive values of the Verigene CDF test were 90.9%, 92.5%, 67.6%, and 98.3%, respectively. Of the 250 concordantly culture-positive specimens, 59 (23.6%) were flagged as "hypervirulent"; 53 were confirmed as ribotype

  10. Identification of irradiated refrigerated poultry with the DNA comet assay

    Science.gov (United States)

    Villavicencio, A. L. C. H.; Araújo, M. M.; Marin-Huachaca, N. S.; Mancini-Filho, J.; Delincée, H.

    2004-09-01

    Food irradiation could make a significant contribution to the reduction of food-borne diseases caused by harmful bacteria such as Salmonella and parasites. In fact these organisms cause an increasing number of diseases and eventually deaths all over the world, also in industrialized countries. Radiation processing has the advantage that in addition to eliminating pathogens, thereby enhancing food safety, it also extends shelf life through destruction of spoilage organisms. The DNA molecule because of its big size is an easy target for ionizing radiation, therefore, changes in DNA offer potential to be used as a detection method for the irradiation treatment. In our study, poultry has been irradiated and changes in DNA analyzed by the Comet Assay. Samples were packed in plastic bags and irradiated. Doses were 0, 1.5, 3.0 and 4.5kGy. Immediately after irradiation the samples were returned to the refrigerator (4°C). Samples were analyzed 1 and 10 days after irradiation. This method proved to be an inexpensive and rapid screening technique for qualitative detection of irradiation treatment.

  11. Comet assay on mice testicular cells

    Directory of Open Access Journals (Sweden)

    Anoop Kumar Sharma

    2015-05-01

    Full Text Available Heritable mutations may result in a variety of adverse outcomes including genetic disease in the offspring. In recent years the focus on germ cell mutagenicity has increased and the “Globally Harmonized System of Classification and Labelling of Chemicals (GHS” has published classification criteria for germ cell mutagens (Speit et al., 2009. The in vivo Comet assay is considered a useful tool for investigating germ cell genotoxicity. In the present study DNA strand breaks in testicular cells of mice were investigated. Different classes of chemicals were tested in order to evaluate the sensitivity of the comet assay in testicular cells. The chemicals included environmentally relevant substances such as Bisphenol A, PFOS and Tetrabrombisphenol A. Statistical power calculations will be presented to aid in the design of future Comet assay studies on testicular cells. Power curves were provided with different fold changes in % tail DNA, different number of cells scored and different number of gels (Hansen et al., 2014. An example is shown in Figure 1. A high throughput version of the Comet assay was used. Samples were scored with a fully automatic comet assay scoring system that provided faster scoring of randomly selected cells.

  12. The micronucleus assay in radiation accidents

    International Nuclear Information System (INIS)

    The cytokinesis-block micronucleus assay in peripheral blood lymphocytes is a standardised and validated technique for bio dosimetry. Automated scoring of micronuclei allows large scale applications as in population triage in case of radiation accidents or malevolent use of radioactive sources. The dose detection limit (95% confidence) of the micronucleus assay for individual dose assessment is restricted to 0.2 Gy but can be decreased to 0.1 Gy by scoring centromeres in micronuclei using fluorescence in situ hybridization (FISH). In the past the micronucleus assay was applied for a number of large scale bio monitoring studies of nuclear power plant workers and hospital workers. Baseline micronucleus frequencies depend strongly on age and gender. The assay was also already used for bio dosimetry of radiation accidents. In a multiple endpoint bio dosimetry study for dose assessment of a worker exposed accidentally in 2003 to X-rays, a good agreement was obtained between dose estimates resulting from the micronucleus assay, the scoring of dicentrics and translocations. Automated scoring of micronuclei in combination with centromere signals, allowing systematic bio dosimetry of exposed populations, remains a challenge for the future.

  13. Potentiometric assay for acid and alkaline phosphatase

    International Nuclear Information System (INIS)

    Simple potentiometric kinetic assay for evaluation of acid and alkaline phosphatase activity has been developed. Enzymatically catalyzed hydrolysis of monofluorophosphate, the simplest inorganic compound containing P-F bond, has been investigated as the basis of the assays. Fluoride ions formed in the course of the hydrolysis of this specific substrate have been detected using conventional fluoride ion-selective electrode based on membrane made of lanthanum fluoride. The key analytical parameters necessary for sensitive and selective detection of both enzymes have been assessed. Maximal sensitivity of the assays was observed at monofluorophosphate concentration near 10-3 M. Maximal sensitivity of acid phosphatase assay was found at pH 6.0, but pH of 4.8 is recommended to eliminate effects from alkaline phosphatase. Optimal pH for alkaline phosphatase assay is 9.0. The utility of the developed substrate-sensor system for determination of acid and alkaline phosphatase activity in human serum has been demonstrated

  14. Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

    Directory of Open Access Journals (Sweden)

    Seiichi Sakamoto

    2012-09-01

    Full Text Available An enzyme-linked immunosorbent assay (ELISA using monoclonal antibody (MAb, Fab antibody, and single-chain variable fragment (scFv antibody has become one of the most promising analytical methods owing to its rapidity, sensitivity, and reliability. Recently, a chimera of green fluorescent protein (GFP with a scFv antibody, named fluobody, was proposed as a probe for an alternative immunosorbent assay; i.e., fluorescence-linked immunosorbent assay (FLISA. In this FLISA, an even more sensitive, simple, and rapid immunoassay can be performed by detecting the highly sensitive fluorophore of GFP that is genetically and directly fused to the scFv antibody. In addition, the time- and cost-consuming secondary antibody reaction and the following enzyme-substrate reaction, necessary for conventional ELISA, can be avoided, making it possible to complete the assay more rapidly. Focusing on naturally occurring bioactive products, fluobody recognizing 1,4-naphthoquinone, plumbagin and triterpenoid saponin, ginsenosides were successfully expressed in Escherichia coli (E. coli and applied to FLISA. The construction, the expression, and the potential use of fluobody in quantitative/qualitative analysis of bioactive natural products are reviewed in this article.

  15. Hepatitis C virus RNA assays: a comparison of SuperQuant and Monitor.

    Science.gov (United States)

    Hadziyannis, E; Hadziyannis, A; Yen-Lieberman, B; Kiwi, M L; Hodnick, S; Spanou, F; Starkey, C; Younossi, Z M

    2001-07-01

    Hepatitis C RNA testing has been used extensively to assess the efficacy of antiviral therapy and has increasingly become an integral part of clinical management of patients with chronic hepatitis C. A variety of commercially available hepatitis C virus (HCV) RNA tests are used to detect HCV RNA qualitatively or quantitatively. These commercial tests have fundamental differences that are reflected on the values they generate. We compared two widely used assays, HCV SuperQuant (SQ) and Amplicor HCV Monitor (M1 and M2), in sera of patients with chronic hepatitis C. A total of 506 sera from 79 patients were tested with both assays. The data were logarithmically transformed and analyzed by linear regression and measurement of agreement. Two hundred thirty-eight sera had HCV RNA values within the dynamic range of both assays. The correlation between the assays was fair, with a correlation coefficient (r) of 0.699. Overall, SQ generated higher values than M1 with a mean difference of 0.558 log (SD = 0.624). One hundred ninety-four (38%) and 121 (24%) of the sera were below the dynamic range of M1 and SQ, respectively. Seventy-three sera, undetectable by M1, were positive by SQ. The Amplicor HCV Monitor 2.0 (M2) was performed in 66 sera. All were positive by SQ and M2, but only 38 were within the dynamic range of M1. The correlations between these tests were good (r = 0.68-0.78), but the agreement was rather poor. In conclusion, this study confirms that both SQ and M2 are more sensitive than M1. Additionally, our results show rather poor agreements between these assays. The recent attempts in standardizing the reporting of these assays should make their results more easily interchangeable. PMID:11418790

  16. Feelings and Intersubjectivity in Qualitative Suicide Research.

    Science.gov (United States)

    Boden, Zoë V R; Gibson, Susanne; Owen, Gareth J; Benson, Outi

    2016-07-01

    In this article, we explore how feelings permeated our qualitative research on suicide. Drawing on phenomenological theory, we argue for the epistemic and ethical importance of the feelings that emerge through research encounters, considering them to be embodied, intersubjective, and multilayered, and requiring careful interpretation through a "reflexivity of feelings." We sketch a tentative framework of the ways that we experienced feelings in our research and give three in-depth examples to illustrate some of the different layers and types of feelings we identified. We reflexively interpret these feelings and their role in our analysis and then discuss some of the ethical and methodological issues related to examining feelings in suicide research, and research more generally. PMID:25794522

  17. Quality in Qualitative Management Accounting Research

    DEFF Research Database (Denmark)

    Nørreklit, Hanne

    2014-01-01

    Purpose: The purpose of this article is to demonstrate how the quality of Qualitative Research in Accounting & Management (QRAM) is manifested through the conceptualization of knowledge about functioning actions that are applicable for local management accounting practices. Design......, the paper has implications for contemporary discussions on doing research that is relevant for practice. Originality/value: The paper provides novel insight into the analysis of quality in management accounting research. Additionally, it provides a framework for reflecting on the accumulation of....../methodology/approach: Drawing on language game theory and pragmatic constructivism, the paper analyzes the "practice doing" embedded in key language games of the case descriptions of three articles on intra-organizational buyer-supplier relations published in QRAM with the aim of revealing how they contribute to the...

  18. Cardinal Direction Relations in Qualitative Spatial Reasoning

    Directory of Open Access Journals (Sweden)

    Chaman L. Sabharwal

    2014-02-01

    Full Text Available Representation and reasoning with spatial information is a fundamental aspect of artificial intelligence. Qualitative methods have become prominent in spatial reasoning. In geophysical explorations, one of the aspects is to determine compass direction between the regions. In this paper, we present an efficient approach to cardinal directions between free form regions. The development is very simple, mathematically sound and can be implemented efficiently. The extension to 3D is seamless; it needs no additional formulation for transition from 2D to 3D.It has no adverse impact on the computational efficiency, as the technique is akin to 2D. This work is directly applicable to geographical information systems for location determination, robot navigation, and spatio-temporal networks databases where direction changes frequently

  19. Qualitative Research in Communication. Introductory Remarks

    Directory of Open Access Journals (Sweden)

    Liz Yeomans

    2016-02-01

    Full Text Available The 20th century’s epistemological turn in social sciences (Denzin & Lincoln, 1994; Punch, 2013 acknowledged the importance of qualitative research methods. The need for this turn was also pointed out by Habermas (1979, who noticed that the way data was collected in social sciences affected the analysis and data interpretation. Research gained a comprehensive character and proposed a phenomenological approach of reality (Guba & Lincoln, 1994; Willig, 2008; Lindlof & Taylor, 2011. Nowadays, we notice a “more confident community of scholars” whose earlier endeavors had “opened up the study of cultures, meanings, symbolic performances, and social practices” (Lindlof & Taylor, 2002, p. xi.

  20. On the foundations of qualitative decision theory

    Energy Technology Data Exchange (ETDEWEB)

    Brafman, R.I. [Univ. of British Columbia, Vancouver (Canada); Tennenholtz, M. [Israel Inst. of Technology, Haifa (Israel)

    1996-12-31

    This paper investigates the foundation of maximin, one of the central qualitative decision criteria, using the approach taken by Savage to investigate the foundation and rationality of classical decision theory. This approach asks {open_quotes}which behaviors could result from the use of a particular decision procedure?{close_quotes} The answer to this question provides two important insights: (1) under what conditions can we employ a particular agent model, and (2) how rational is a particular decision procedure. Our main result is a constructive representation theorem in the spirit of Savage`s result for expected utility maximization, which uses two choice axioms to characterize the maximin criterion. These axioms characterize agent behaviors that can be modeled compactly using the maximin model, and, with some reservations, indicate that maximin is a reasonable decision criterion.

  1. Nurse researchers in corrections: a qualitative study.

    Science.gov (United States)

    Ferszt, Ginette G; Hickey, Joyce

    2013-01-01

    The United States has more people, per capita, in prisons and jails than any other country in the world. Because the prison population is largely composed of people who have been economically and socially disadvantaged, a very high percentage enter correctional facilities in poor health. Because of the large concentrated numbers of women, men, and youth in prisons and jails, an exceptional opportunity exists for nurses and other researchers to conduct creative and innovative research to improve the health care of this hard-to-reach population. The purpose of this study was to explore the experiences of nurse researchers who have been successful in conducting studies in different correctional settings. A qualitative descriptive design was used, and telephone interviews with 16 participants were completed. The participants described how they established credibility, the challenges they faced, and the unexpected personal and professional rewards they received. Recommendations for potential researchers will hopefully lead to an increase in research with this invisible population. PMID:24256982

  2. QUALITATIVE MARKETING RESEARCH REGARDING THE MULTICHANNEL DISTRIBUTION

    Directory of Open Access Journals (Sweden)

    CĂTĂLIN NICOLAE BULGĂREA

    2011-01-01

    Full Text Available Market research can be defined as an “active form through which, by means of different concepts, methods and techniques of scientific investigation, is carried out, systematically, the specification, the measurement, the collection, the analysis and the objective interpretation of marketing information for the management of the economic unit, in order to know better the company’s environment, to identify the opportunities, to evaluate the alternatives of marketing actions and their effects. The qualitative research seeks answers to questions like: “why?” and “how?”, in order to find the root causes of consumers' attitudes, motives, behaviours, preferences and opinions and also the subjective, emotional or unconscious elements behind them.

  3. A qualitative multiresolution model for counterterrorism

    Science.gov (United States)

    Davis, Paul K.

    2006-05-01

    This paper describes a prototype model for exploring counterterrorism issues related to the recruiting effectiveness of organizations such as al Qaeda. The prototype demonstrates how a model can be built using qualitative input variables appropriate to representation of social-science knowledge, and how a multiresolution design can allow a user to think and operate at several levels - such as first conducting low-resolution exploratory analysis and then zooming into several layers of detail. The prototype also motivates and introduces a variety of nonlinear mathematical methods for representing how certain influences combine. This has value for, e.g., representing collapse phenomena underlying some theories of victory, and for explanations of historical results. The methodology is believed to be suitable for more extensive system modeling of terrorism and counterterrorism.

  4. Qualitative dynamics of wavepackets in turbulent jets

    CERN Document Server

    Semeraro, Onofrio; Pastur, Luc; Jordan, Peter

    2016-01-01

    It has long been established that turbulent jets comprise large-scale coherent structures, now more commonly referred to as "wavepackets". These structures exhibit a remarkable spatio-temporal organisation, despite turbulence. In this work we analyse, from a qualitative point of view, the temporal dynamics of axisymmetric wavepackets educed, experimentally, from subsonic iso-thermal jets. We use the data presented by, where time-series of the wavepackets are extracted at different streamwise locations. A thorough analysis is performed, statistical tools are used for estimating the embedding and correlation dimensions characterising the dynamical system. System identification is used for computing nonlinear surrogate models. Finally, control-oriented linear models are computed. The goal of the contribution is to assess the extent to which non-linear models are necessary, or appropriate, for description of the temporal wave-packet dynamics and to provide a complementary perspective to the current modelling.

  5. Development of an integrated assay facility

    International Nuclear Information System (INIS)

    The I.R.I.S. concept proposed the use of passive examination and active interrogation techniques in an integrated assay facility. A linac would generate the interrogating gamma and neutron beams. Insufficiently detailed knowledge about active neutron and gamma interrogation of 500 litre drums of cement immobilised intermediate level waste led to a research programme which is now in its main experimental stage. Measurements of interrogation responses are being made using simulated waste drums containing actinide samples and calibration sources, in an experimental assay assembly. Results show that responses are generally consistent with theory, but that improvements are needed in some areas. A preliminary appraisal of the engineering and economic aspects of integrated assay shows that correct operational sequencing is required to achieve the short cycle time needed for high throughput. The main engineering features of a facility have been identified

  6. Nuclear Resonance Fluorescence for Materials Assay

    Energy Technology Data Exchange (ETDEWEB)

    Quiter, Brian J.; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

    2009-06-29

    This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX?s photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

  7. Nuclear Resonance Fluorescence for Materials Assay

    Energy Technology Data Exchange (ETDEWEB)

    Quiter, Brian; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

    2009-06-05

    This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX's photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

  8. Monitoring environmental exposures with semen assays

    International Nuclear Information System (INIS)

    Semen studies in humans and animals have yielded extensive and compelling evidence that sperm can be used to assess reproductive potential and diagnose pathology. More recent studies on mutagens and carcinogens both at this and other laboratories suggest that a combination of mouse and human assays can be an efficient, effective approach to monitoring for reproductive hazards in the environment. We are investigating the potential of using variability in sperm morphology and DNA content to quantify and monitor the effects of environmental agents on the human testes. Here we review the status of human and mouse assays for environmental surveillance, discuss the genetic and fertility implications of chemically induced semen changes, and describe the high-speed flow methods being developed to automate sperm assays

  9. Comet assay on tetraploid yeast cells

    DEFF Research Database (Denmark)

    Rank, Jette; Syberg, Kristian; Jensen, Klara

    2009-01-01

    Tetraploid yeast cells (Saccharomyces cerevisiae) were used in the comet assay with the intention of developing a new, fast and easy assay for detecting environmental genotoxic agents without using higher organisms. Two DNA-damaging chemicals, H2O2 and acrylamide, together with wastewater from...... three municipal treatment plants were tested for their effect on the yeast-cell DNA. The main problem with using yeast in the comet assay is the necessity to degrade the cell wall. This was achieved by using Zymolase 100 T twice during the procedure, since Zymolase 20 T did not open the cell wall...... causing significant DNA damage was 20 μM for H2O2 and 200 mg/l for acrylamide. Tertiary-treated wastewater from the outlets of three municipal wastewater-treatment plants was tested, but did not cause DNA damage. Even though it is possible to produce comets with tetraploid yeast cells, the amount of DNA...

  10. Nuclear Resonance Fluorescence for Materials Assay

    International Nuclear Information System (INIS)

    This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has been performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX?s photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

  11. Nanoparticles for Use in Enzyme Assays.

    Science.gov (United States)

    Kim, Young-Pil; Kim, Hak-Sung

    2016-02-01

    Nanoparticles (NPs) have created new ways to enhance the performance of classical biosensors in analytical sciences. NPs with unprecedented physiochemical properties can serve both as excellent carriers of bioreceptors and as signal enhancers, leading to improved assay platforms with high sensitivity and selectivity. Because enzymes play central roles in many cellular functions, specific and precise assays of their functions are of great significance in medical science and biotechnology. Here we review recent advances in NP-based biosensors and their use in enzyme assays. With fast and specific responses to enzyme-mediated reactions, NPs transduce and amplify the initial responses into various types of signals, such as electrochemical, optical and magnetic ones. Translation of their potential should lead to functionalized NPs finding wide applications in diagnostics, drug development and biotechnology. PMID:26662229

  12. The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.

    OpenAIRE

    Bas-jan Van Der Leede

    2015-01-01

    In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance. In this presentation we want to give insight into the circumstances in vivo comet assay is deployed in a Genetic Toxicology Department of a pharmaceutical company. As the in vivo comet assay is a salvage assay, it means that some events have occu...

  13. Method validation and clinical utility of chromogenic factor VIII assay compared to one-stage assay.

    Science.gov (United States)

    Gouws, Wilmare; Botha, Elsabie; Visser, Adele

    2014-01-01

    The chromogenic FVIII assay is currently considered the gold standard for quantitation of factor VIII levels in both haemophilia A patients and as part of screening for thrombophilia. A method validation and evaluation of clinical utility within a routine diagnostic laboratory was undertaken by comparing the currently used one-stage assay to a commercially available chromogenic assay (Siemens, Johannesburg, South Africa). In total, 60 samples were included in this study to encompass the whole diagnostic range of the assay. Both low and high values showed very good correlation on linear regression analysis with correlation coeffients of 0.949 and 0.888 respectively. However, the lower detection limit of the Siemens Chromogenic assay was 1.5 IU/dL rendering it impossible to utilize in the setting of classifying a haemophilia A patient in terms of disease severity. Although the Siemens FVIII chromogenic assay shows excellent correlation to the currently used one-stage assay, the relatively high detection limit restrict implementation as a stand-alone assay in a routine diagnostic laboratory. PMID:23504571

  14. Relationship between the radioisotopic footpad assay and other immunological assays in tumor bearing rats

    International Nuclear Information System (INIS)

    KMT-17, a fibrosarcoma induced by 3-methylcholanthrene in a WKA rat, is a sensitive tumor to various kinds of immunological assays and is a suitable model tumor for the study of the immune status in tumor bearing hosts. The antitumor immune response of KMT-17 bearing rats was studied by a radioisotopic footpad assay (FPA) in comparison with other in vivo and in vitro assays. Delayed hypersensitivity to tumor antigens measured by the FPA was observed from the 8th day after transplantation of KMT-17 cells, reached a peak on the 12 - 15th day, and then declined in the late stage on the 17th day. The kinetics of the FPA correlated well with those of an in vivo Winn assay and of an in vitro lymphocyte cytotoxicity assay (51Cr-release assay). The appearance of an antitumor antibody detected by a complement dependent cytotoxicity test also correlated well with the kinetics of the FPA. A growth inhibition assay (GIA) for non-specific cell-mediated immunity also showed similar kinetics to that of the FPA. The delayed hypersensitivity footpad reaction to tumor cell extracts measured by this FPA was tumor-specific. These results suggest that the FPA is a simple and reliable in vivo assay for evaluating antitumor immunity in tumor bearing hosts. (author)

  15. Instructions for Uploading Data to the Assay Portal - Instructions for Uploading Data to the Assay Portal

    Science.gov (United States)

    This document provides instructions for configuring and uploading data files to the CPTAC Assay Portal. It is divided into sections, with an overview checklist provided at the end. If help is needed at any stage of the process, please use the support page: https://assays.cancer.gov/support/

  16. Bicinchoninic acid (BCA) assay in low volume.

    Science.gov (United States)

    Bainor, Anthony; Chang, Lyra; McQuade, Thomas J; Webb, Brian; Gestwicki, Jason E

    2011-03-15

    The BCA assay is a colorimetric method for estimating protein concentration. In 96-well plates, the relationship between protein content and absorbance is nearly linear over a wide range; however, performance is reduced in lower volume. To overcome this limitation, we performed the BCA assays in opaque, white 384-well plates. These plates emit fluorescence between 450-600 nm when excited at 430 nm; thus, their fluorescence is quenched by the BCA chromophore (λ(max) 562 nm). This arrangement allowed accurate determination of protein content using only 2 μL of sample. Moreover, soluble flourescein could replace the white plates, creating a homogenous format. PMID:21078286

  17. A sensitive assay for Staphylococcus aureus nucleases

    International Nuclear Information System (INIS)

    A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured [3H] thymidine labelled DNA from E.coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleases elaborated by different strains of S. aureus as well as for determining the extent of contamination of S. aureus in food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate. (author). 26 refs., 3 figs ., 3 tabs

  18. Sensitive radioenzymatic assay for catechol drugs

    International Nuclear Information System (INIS)

    This assay measures picogram quantities of catechol drugs and endogenous catecholamines in body tissues and fluids. The catechols are converted to their 3H-O-methyl metabolites during incubation with 3H-S-adenosylmethionine then separated by solvent extraction and thin-layer chromatography. Most drugs containing the catechol structure can be radiolabeled and separated from norepinephrine and epinephrine by this technique to provide simultaneous measurement of endogenous and exogenously administered catechols. The disposition of isoproterenol in tissues and fluids of man and experimental animals is measured to illustrate the utility of this assay. The reactivity of several commonly administered catechol drugs with COMT is described and the possible implications discussed

  19. Detection of Eperythrozoon wenyoni by PCR assay

    Institute of Scientific and Technical Information of China (English)

    Jian WANG; Yutao ZHU; Jianhua QIN; Fumei ZHANG; Yuelan ZHAO

    2009-01-01

    The objective of this research was to develop a detection method for Eperythrozoon wenyoni infection using polymerase chain reaction (PCR) assay technique. A pair of primers was designed and synthesized according to the conservative sequence 16S rRNA. The PCR assay was performed with the primers. A 985-bp fragment was amplified by using PCR. The amplified fragments with the expected size were identified by EcoR I restriction digestion. The crossing-reaction, specific-reaction and duplicate-reaction indicated that the PCR method is a specific, sensitive, fast and effective method for diagnosing E. Wenyoni infection at group level.

  20. Elements of nondestructive assay (NDA) technology

    International Nuclear Information System (INIS)

    A thorough introduction to nondestructive assay methods and instruments as they are applied to nuclear safeguards is presented. The general principles and major applications of NDA are discussed and situations in which NDA is particularly useful for nuclear safeguards purposes are described. Various passive and active γ-ray and neutron methods are examined and assay situations particularly suited to γ-ray techniques, or to neutron techniques are identified. The role of calorimetry in the NDA of plutonium-bearing materials is also discussed. The advantages and disadvantages of various NDA methods for different types of nuclear materials are mentioned

  1. Serum Aminoglycoside Assay by Enzyme-Mediated Immunoassay (EMIT): Correlation with Radioimmunoassay, Fluoroimmunoassay, and Acetyltransferase and Microbiological Assays

    OpenAIRE

    White, L O; Scammell, L. M.; Reeves, D S

    1981-01-01

    Enzyme-mediated immunoassay (EMIT) serum aminoglycoside assay results were accurate and precise and correlated well with radioimmunoassay, fluoroimmunoassay, and acetyltransferase and microbiological assay determinations.

  2. Qualitative description - the poor cousin of health research?

    DEFF Research Database (Denmark)

    Neergaard, Mette Asbjørn; Olesen, Frede; Andersen, Rikke Sand;

    2009-01-01

    BACKGROUND: The knowledge and use of qualitative description as a qualitative research approach in health services research is limited.The aim of this article is to discuss the potential benefits of a qualitative descriptive approach, to identify its strengths and weaknesses and to provide examples...... of use. DISCUSSION: Qualitative description is a useful qualitative method in much medical research if you keep the limitations of the approach in mind. It is especially relevant in mixed method research, in questionnaire development and in research projects aiming to gain firsthand knowledge of...... patients', relatives' or professionals' experiences with a particular topic. Another great advantage of the method is that it is suitable if time or resources are limited. SUMMARY: As a consequence of the growth in qualitative research in the health sciences, researchers sometimes feel obliged to designate...

  3. Travelling Methods: Tracing the Globalization of Qualitative Communication Research

    Directory of Open Access Journals (Sweden)

    Bryan C. Taylor

    2016-05-01

    Full Text Available Existing discussion of the relationships between globalization, communication research, and qualitative methods emphasizes two images: the challenges posed by globalization to existing communication theory and research methods, and the impact of post-colonial politics and ethics on qualitative research. We draw in this paper on a third image – qualitative research methods as artifacts of globalization – to explore the globalization of qualitative communication research methods. Following a review of literature which tentatively models this process, we discuss two case studies of qualitative research in the disciplinary subfields of intercultural communication and media audience studies. These cases elaborate the forces which influence the articulation of national, disciplinary, and methodological identities which mediate the globalization of qualitative communication research methods.

  4. Travelling Methods: Tracing the Globalization of Qualitative Communication Research

    Directory of Open Access Journals (Sweden)

    Bryan C. TAYLOR

    2013-12-01

    Full Text Available Existing discussion of the relationships between globalization, communication research, and qualitative methods emphasizes two images: the challenges posed by globalization to existing communication theory and research methods, and the impact of post-colonial politics and ethics on qualitative research. We draw in this paper on a third image – qualitative research methods as artifacts of globalization – to explore the globalization of qualitative communication research methods. Following a review of literature which tentatively models this process, we discuss two case studies of qualitative research in the disciplinary subfields of intercultural communication and media audience studies. These cases elaborate the forces which influence the articulation of national, disciplinary, and methodological identities which mediate the globalization of qualitative communication research methods.

  5. Demonstration of a visual cell-based assay for screening glucose transporter 4 translocation modulators in real time

    Indian Academy of Sciences (India)

    Maleppillil Vavachan Vijayakumar; Amrendra Kumar Ajay; Manoj Kumar Bhat

    2010-12-01

    Insulin-stimulated translocation of glucose transporter 4 (GLUT4) to cell membrane leading to glucose uptake is the rate-limiting step in diabetes. It is also a defined target of antidiabetic drug research. Existing GLUT4 translocation assays are based on time-consuming immunoassays and are hampered by assay variability and low sensitivity. We describe a real-time, visual, cell-based qualitative GLUT4 translocation assay using CHO-HIRc-myc-GLUT4eGFP cells that stably express myc- and eGFP-tagged GLUT4 in addition to human insulin receptor (HIRc). GLUT4 translocation is visualized by live cell imaging based on GFP fluorescence by employing a cooled charge-coupled device camera attached to a fluorescent microscope. This video imaging method and further quantitative analysis of GLUT4 on the cell membrane provide rapid and foolproof visual evidence that this method is suitable for screening GLUT4 translocation modulators.

  6. Evaluation of four endogenous reference genes and their real-time PCR assays for common wheat quantification in GMOs detection.

    Directory of Open Access Journals (Sweden)

    Huali Huang

    Full Text Available Proper selection of endogenous reference genes and their real-time PCR assays is quite important in genetically modified organisms (GMOs detection. To find a suitable endogenous reference gene and its real-time PCR assay for common wheat (Triticum aestivum L. DNA content or copy number quantification, four previously reported wheat endogenous reference genes and their real-time PCR assays were comprehensively evaluated for the target gene sequence variation and their real-time PCR performance among 37 common wheat lines. Three SNPs were observed in the PKABA1 and ALMT1 genes, and these SNPs significantly decreased the efficiency of real-time PCR amplification. GeNorm analysis of the real-time PCR performance of each gene among common wheat lines showed that the Waxy-D1 assay had the lowest M values with the best stability among all tested lines. All results indicated that the Waxy-D1 gene and its real-time PCR assay were most suitable to be used as an endogenous reference gene for common wheat DNA content quantification. The validated Waxy-D1 gene assay will be useful in establishing accurate and creditable qualitative and quantitative PCR analysis of GM wheat.

  7. Qualitative and Quantitative Detection of Botulinum Neurotoxins from Complex Matrices: Results of the First International Proficiency Test

    Directory of Open Access Journals (Sweden)

    Sylvia Worbs

    2015-11-01

    Full Text Available In the framework of the EU project EQuATox, a first international proficiency test (PT on the detection and quantification of botulinum neurotoxins (BoNT was conducted. Sample materials included BoNT serotypes A, B and E spiked into buffer, milk, meat extract and serum. Different methods were applied by the participants combining different principles of detection, identification and quantification. Based on qualitative assays, 95% of all results reported were correct. Successful strategies for BoNT detection were based on a combination of complementary immunological, MS-based and functional methods or on suitable functional in vivo/in vitro approaches (mouse bioassay, hemidiaphragm assay and Endopep-MS assay. Quantification of BoNT/A, BoNT/B and BoNT/E was performed by 48% of participating laboratories. It turned out that precise quantification of BoNT was difficult, resulting in a substantial scatter of quantitative data. This was especially true for results obtained by the mouse bioassay which is currently considered as “gold standard” for BoNT detection. The results clearly demonstrate the urgent need for certified BoNT reference materials and the development of methods replacing animal testing. In this context, the BoNT PT provided the valuable information that both the Endopep-MS assay and the hemidiaphragm assay delivered quantitative results superior to the mouse bioassay.

  8. Qualitätsmanagement im ambulanten Bereich – Teil 2

    OpenAIRE

    Czypionka, Thomas; Sigl, Clemens; Berger, Michael; Kronemann, Frank; Reiss, Miriam

    2014-01-01

    Diese Ausgabe des HSW bildet die Fortsetzung der Ausgabe I-A 2014 zum Thema Qualitätsmanagement im ambulanten Bereich. Wurden im ersten Teil eine theoretische Einordnung und Instrumente des Qualitätsmanagement vorgestellt, so wird in diesem Teil auf internationale Qualitätsmanagement-Systeme eingegangen und abschließend ein Ausblick für Österreich gegeben.

  9. How to state general qualitative facts in Psychology?

    OpenAIRE

    Vautier, Stéphane

    2013-01-01

    In what form should qualitative psychological statements have to be in order to be general and falsifiable? I show how basic qualitative measurement systems can be defined that allow for the detection and testing of general relational facts of the if-then form in a well-defined range of potential qualitative, static or dynamic, multivariate observations. The formal framework permits the pointing out of general research problems falling under the scope of empirical investigation.

  10. QUALITATIVE OPERATION INDICES OF TRANSPORT COMPLEX OF THE RUSSIAN FEDERATION

    OpenAIRE

    I. N. Pugachev; Yu. I. Kulikov; V. N. Sediukevich

    2015-01-01

    The paper considers existing qualitative indices of transport services. Indices for evaluation of qualitative operation indices of transport complex have been proposed in the paper. Special attention has been devoted to interaction and coordination of various types of transport as the highest level of qualitative operation of transport complexes. Operational coordination of transport facilities is considered as introduction of modern management, information and communication systems while usi...

  11. Joint association analysis of bivariate quantitative and qualitative traits

    OpenAIRE

    2011-01-01

    Univariate genome-wide association analysis of quantitative and qualitative traits has been investigated extensively in the literature. In the presence of correlated phenotypes, it is more intuitive to analyze all phenotypes simultaneously. We describe an efficient likelihood-based approach for the joint association analysis of quantitative and qualitative traits in unrelated individuals. We assume a probit model for the qualitative trait, under which an unobserved latent variable and a presp...

  12. Enhancing the quality and credibility of qualitative analysis.

    OpenAIRE

    Patton, M Q

    1999-01-01

    Varying philosophical and theoretical orientations to qualitative inquiry remind us that issues of quality and credibility intersect with audience and intended research purposes. This overview examines ways of enhancing the quality and credibility of qualitative analysis by dealing with three distinct but related inquiry concerns: rigorous techniques and methods for gathering and analyzing qualitative data, including attention to validity, reliability, and triangulation; the credibility, comp...

  13. An Introduction to Qualitative Research for Food and Nutrition Professionals.

    OpenAIRE

    Harris, Jeffrey E.; Philip M. Gleason; Sheean, Patricia M.; Carol Boushey; Beto, Judith A; Barbara Bruemmer

    2009-01-01

    This article defines qualitative research as applied in the field of dietetics to increase knowledge and competency in evaluating this type of research. The authors explain the design of qualitative studies, explore congruence with quantitative research, and provide examples of applications in dietetics, stressing the importance of ensuring validity and reliability of qualitative measures. The article aims to help food and nutrition professionals add to the body of peer-reviewed, dietetics-re...

  14. Pragmatism vs interpretivism in qualitative information systems research

    OpenAIRE

    Goldkuhl, Göran

    2012-01-01

    Qualitative research is often associated with interpretivism, but alternatives do exist. Besides critical research and sometimes positivism, qualitative research in information systems can be performed following a paradigm of pragmatism. This paradigm is associated with action, intervention and constructive knowledge. This paper has picked out interpretivism and pragmatism as two possible and important research paradigms for qualitative research in information systems. It clarifies each parad...

  15. Endoproteolytic activity assay in malting barley

    Directory of Open Access Journals (Sweden)

    Blanca Gómez Guerrero

    2013-12-01

    Full Text Available Hydrolysis of barley proteins into peptides and amino acids is one of the most important processes during barley germination.The degradation of the endosperm stored proteins facilitates water and enzyme movements, enhances modification, liberates starch granules and increases soluble amino nitrogen. Protease activity is the result of the activities of a mixture of exo- and endo-proteases. The barley proteins are initially solubilized by endo-proteases and the further by exo-proteases. Four classes of endo-proteases have been described: serine-proteases, cysteine-proteases, aspartic-proteases and metallo-proteases. The objective of this work was to develop a rapid and colorimetric enzymatic assay to determine the endo-proteolytic activity of the four endo-protease classes using two different substrates: azo-gelatin and azo-casein. Optimum conditions for the assays such as: pH,reaction time and temperature and absorbance scale were determined. Azo-gelatin presented several difficulties in standardizing an “in solution” assay. On the other hand, azo-casein allowed standardization of the assay for the four enzyme classes to produce consistent results. The endo-proteoteolytic method developed was applied to determine the endo-protease activity in barley, malt and wort.

  16. A novel fluorescent assay for sucrose transporters

    Directory of Open Access Journals (Sweden)

    Gora Peter J

    2012-04-01

    Full Text Available Abstract Background We have developed a novel assay based on the ability of type I sucrose uptake transporters (SUTs to transport the fluorescent coumarin β-glucoside, esculin. Budding yeast (Saccharomyces cerevisiae is routinely used for the heterologous expression of SUTs and does not take up esculin. Results When type I sucrose transporters StSUT1 from potato or AtSUC2 from Arabidopsis were expressed in yeast, the cells were able to take up esculin and became brightly fluorescent. We tested a variety of incubation times, esculin concentrations, and buffer pH values and found that for these transporters, a 1 hr incubation at 0.1 to 1 mM esculin at pH 4.0 produced fluorescent cells that were easily distinguished from vector controls. Esculin uptake was assayed by several methods including fluorescence microscopy, spectrofluorometry and fluorescence-activiated cell sorting (FACS. Expression of the type II sucrose transporter OsSUT1 from rice did not result in increased esculin uptake under any conditions tested. Results were reproduced successfully in two distinct yeast strains, SEY6210 (an invertase mutant and BY4742. Conclusions The esculin uptake assay is rapid and sensitive and should be generally useful for preliminary tests of sucrose transporter function by heterologous expression in yeast. This assay is also suitable for selection of yeast showing esculin uptake activity using FACS.

  17. Capillary Electrophoresis Analysis of Conventional Splicing Assays

    DEFF Research Database (Denmark)

    de Garibay, Gorka Ruiz; Acedo, Alberto; García-Casado, Zaida;

    2014-01-01

    Rare sequence variants in "high-risk" disease genes, often referred as unclassified variants (UVs), pose a serious challenge to genetic testing. However, UVs resulting in splicing alterations can be readily assessed by in vitro assays. Unfortunately, analytical and clinical interpretation of thes...

  18. Homogeneous screening assay for human tankyrase.

    Science.gov (United States)

    Narwal, Mohit; Fallarero, Adyary; Vuorela, Pia; Lehtiö, Lari

    2012-06-01

    Tankyrase, a member of human PARP protein superfamily, catalyzes a covalent post-translational modification of substrate proteins. This modification, poly(ADP-ribos)ylation, leads to changes in protein interactions and modifies downstream signaling events. Tankyrase 1 is a potential drug target due to its functions in telomere homeostasis and in Wnt signaling. We describe here optimization and application of an activity-based homogenous assay for tankyrase inhibitors in a high-throughput screening format. The method measures the consumption of substrate by the chemical conversion of the remaining NAD(+) into a stable fluorescent condensation product. Conditions were optimized to measure the enzymatic auto-modification of a recombinant catalytic fragment of tankyrase 1. The fluorescence assay is inexpensive, operationally easy and performs well according to the statistical analysis (Z'= 0.7). A validatory screen with a natural product library confirmed suitability of the assay for finding new tankyrase inhibitors. Flavone was the most potent (IC(50)=325 nM) hit from the natural compounds. A flavone derivative, apigenin, and isopropyl gallate showed potency on the micromolar range, but displayed over 30-fold selectivity for tankyrase over the studied isoenzymes PARP1 and PARP2. The assay is robust and will be useful for screening new tankyrase inhibitors. PMID:22357873

  19. Verification of prior measurements by nondestructive assay

    International Nuclear Information System (INIS)

    Emphasis in this report is placed on the factors affecting nondestructive assay (NDA) measurement accuracies and the selection of the appropriate NDA techniques for certain measurements based on these factors. Factors affecting measurement accuracies are operational characteristics, isotopic composition of SNM sample, response dependence on the SNM and matrix materials within sample, and composition and dimension of the container

  20. Radioligand assay for biotin in liver tissues

    International Nuclear Information System (INIS)

    A radioligand assay for biotin in liver tissue is described. 3H-biotin is used as tracer and avidin as binder. The biotin-loaded avidin is separated from free biotin on dextran-coated charcoal, which leaves the avidin-biotin complex in the supernatant liquid. Thus, the avidin-biotin complex can easily be utilized for determination of the radioactivity. Calibration with known additions of biotin in the range 0.25-8.0 ng per assay sample yields a linear logit-log plot. The biotin is extracted from liver tissues by enzymatic proteolysis with papain. This treatment is optimized to liberate the bound forms of the vitamin. Microbiological parallel assays with Lactobacillus plantarum were in good agreement with the radioligand assay giving a regression coefficient of 0.974(n=44). The coefficient of variation was found to be 4.2% in the range 500-1200 ng of biotin per g of liver tissue (n=46). The method is simple and reliable and allows the simultaneous analysis of a considerable number of samples. (Auth.)

  1. Enzymatic assay for methotrexate in erythrocytes

    DEFF Research Database (Denmark)

    Schrøder, H; Heinsvig, E M

    1985-01-01

    Methotrexate (MTX) accumulates in erythrocytes in MTX-treated patients. We present a modified enzymatic assay measuring MTX concentrations between 10 and 60 nmol/l in erythrocytes, adapted for a centrifugal analyser (Cobas Bio). About 40 patient's samples could be analysed within 1 h. The detection...

  2. Antibacterial effect of protamine assayed by impedimetry

    DEFF Research Database (Denmark)

    Johansen, Charlotte; Gill, T.; Gram, Lone

    1995-01-01

    Impedimetric measurements were used to assay the antibacterial effect of protamine. A good linear correlation between the impedance detection time and the initial cell counts was obtained (r = 0 . 99, n = 2). As basic peptides may cause clumping of cells, this correlation curve was used when...

  3. Benzodiazepine Synthesis and Rapid Toxicity Assay

    Science.gov (United States)

    Fletcher, James T.; Boriraj, Grit

    2010-01-01

    A second-year organic chemistry laboratory experiment to introduce students to general concepts of medicinal chemistry is described. Within a single three-hour time window, students experience the synthesis of a biologically active small molecule and the assaying of its biological toxicity. Benzodiazepine rings are commonly found in antidepressant…

  4. Advanced analysis techniques for uranium assay

    International Nuclear Information System (INIS)

    Uranium has a negligible passive neutron emission rate making its assay practicable only with an active interrogation method. The active interrogation uses external neutron sources to induce fission events in the uranium in order to determine the mass. This technique requires careful calibration with standards that are representative of the items to be assayed. The samples to be measured are not always well represented by the available standards which often leads to large biases. A technique of active multiplicity counting is being developed to reduce some of these assay difficulties. Active multiplicity counting uses the measured doubles and triples count rates to determine the neutron multiplication (f4) and the product of the source-sample coupling ( C ) and the 235U mass (m). Since the 35U mass always appears in the multiplicity equations as the product of Cm, the coupling needs to be determined before the mass can be known. A relationship has been developed that relates the coupling to the neutron multiplication. The relationship is based on both an analytical derivation and also on empirical observations. To determine a scaling constant present in this relationship, known standards must be used. Evaluation of experimental data revealed an improvement over the traditional calibration curve analysis method of fitting the doubles count rate to the 235Um ass. Active multiplicity assay appears to relax the requirement that the calibration standards and unknown items have the same chemical form and geometry.

  5. Plants Bioassays: Comet Assay on Higher Plants

    Czech Academy of Sciences Publication Activity Database

    Mukherjee, A.; Gichner, Tomáš

    Houston: Studium Press, 2009 - (Sampietro, D.; Narwal, S.), s. 97-108 ISBN 1-933699-42-6 R&D Projects: GA ČR GA521/05/0500 Institutional research plan: CEZ:AV0Z50380511 Keywords : Comet assay * DNA damage * Plants Subject RIV: EB - Genetics ; Molecular Biology

  6. A radiometric assay for HIV-1 protease

    International Nuclear Information System (INIS)

    A rapid, high-throughput radiometric assay for HIV-1 protease has been developed using ion-exchange chromatography performed in 96-well filtration plates. The assay monitors the activity of the HIV-1 protease on the radiolabeled form of a heptapeptide substrate, [tyrosyl-3,5-3H]Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, which is based on the p17-p24 cleavage site found in the viral polyprotein substrate Pr55gag. Specific cleavage of this uncharged heptapeptide substrate by HIV-1 protease releases the anionic product [tyrosyl-3,5-3H]Ac-Ser-Gln-Asn-Tyr, which is retained upon minicolumns of the anion-exchange resin AG1-X8. Protease activity is determined from the recovery of this radiolabeled product following elution with formic acid. This facile and highly sensitive assay may be utilized for steady-state kinetic analysis of the protease, for measurements of enzyme activity during its purification, and as a routine assay for the evaluation of protease inhibitors from natural product or synthetic sources

  7. Radioreceptor assay for epidermal growth factor

    International Nuclear Information System (INIS)

    An established cell line of human lung fibroblasts with a high number of surface receptors for mouse epidermal growth factor (mEGF) was used to deveop a simple and highly sensitive radioreceptor assay for EGF. 125I-Labeled mEGF competed mole for mole with unlabeled mEGF for specific receptors. Optimal range for discriminating EGF concentrations in body fluids and tissue extracts by a competitive binding assay was between 5 and 100 ng/ml. Interassay correlation of variation was 8.47% and the recovery of highly purified mEGF added to serum and urine samples was greater than 95%. Human serum and amniotic fluids contained about 24 and 4 ng/ml, respectively, of mEGF equivalents. Concentrations of mEGF in mouse urine and serum were highly variable and were 2- to 10-fold greater than that previously detected by radioimmune assay. Hypophysectomy nearly abolished submaxillary mEGF content in both male and female mice, but testosterone treatment of hypophysectomized animals restored normal concentrations of mEGF to the glands. mEGF added to culture medium disappeared with time as a function of the number of cellular EGF receptors indicating cellular degradation of the growth factor. The radioreceptor assay for EGF is based on the close biologic relationship between the cell receptor site and the native hormone and should prove to be a useful complementary tool to characterize the physiological role of EGF

  8. Nondestructive assay of boxed radioactive waste

    International Nuclear Information System (INIS)

    This paper describes the problems related to the nondestructive assay (NDA) of boxed radioactive waste at the Hanford Site and how Westinghouse Hanford company (WHC) is solving the problems. The waste form and radionuclide content are described. The characteristics of the combined neutron and gamma-based measurement system are described

  9. The Four Methods of Qualitative Research in Education

    Institute of Scientific and Technical Information of China (English)

    孙慧

    1996-01-01

    "Research is a systematic investigation to find answers to a problem" ( Introduction to Research Methods in Deucation 1990:1). Educational research, like research in the other areas, includes quantitative and qualitative methods, both of which are equally important, but serve different roles. Qualitative research focuses on people' s behaviour, attitudes, opinions and so on. Then, what is the nature of qualitative research? Upon what assumption is it based? This paper will try to address four qualitative methods - survey, case study, action research and ethnography to look at how each of them works in educational research.

  10. The phenomenological method in qualitative psychology and psychiatry

    Directory of Open Access Journals (Sweden)

    Magnus Englander

    2016-03-01

    Full Text Available This article will closely examine the phenomenological method as applied to qualitative inquiry in psychology and psychiatry. In a critical comparison between Amedeo Giorgi's and Larry Davidson's qualitatively methods, conclusions were drawn with regard to how different kinds of qualitative inquiry are possible while remaining faithful to Husserlian philosophical foundations. Utilizing Lester Embree's recent articulation of how Husserl's method of the epochē can be disclosed as specific to a discipline, varieties of these two qualitative methods were seen in their relation to the original scientific aim instigated by the developer.

  11. The phenomenological method in qualitative psychology and psychiatry

    Science.gov (United States)

    Englander, Magnus

    2016-01-01

    This article will closely examine the phenomenological method as applied to qualitative inquiry in psychology and psychiatry. In a critical comparison between Amedeo Giorgi's and Larry Davidson's qualitatively methods, conclusions were drawn with regard to how different kinds of qualitative inquiry are possible while remaining faithful to Husserlian philosophical foundations. Utilizing Lester Embree's recent articulation of how Husserl's method of the epochē can be disclosed as specific to a discipline, varieties of these two qualitative methods were seen in their relation to the original scientific aim instigated by the developer. PMID:26968361

  12. Reliability and Validity of Qualitative and Operational Research Paradigm

    Directory of Open Access Journals (Sweden)

    Muhammad Bashir

    2008-01-01

    Full Text Available Both qualitative and quantitative paradigms try to find the same result; the truth. Qualitative studies are tools used in understanding and describing the world of human experience. Since we maintain our humanity throughout the research process, it is largely impossible to escape the subjective experience, even for the most experienced of researchers. Reliability and Validity are the issue that has been described in great deal by advocates of quantitative researchers. The validity and the norms of rigor that are applied to quantitative research are not entirely applicable to qualitative research. Validity in qualitative research means the extent to which the data is plausible, credible and trustworthy; and thus can be defended when challenged. Reliability and validity remain appropriate concepts for attaining rigor in qualitative research. Qualitative researchers have to salvage responsibility for reliability and validity by implementing verification strategies integral and self-correcting during the conduct of inquiry itself. This ensures the attainment of rigor using strategies inherent within each qualitative design, and moves the responsibility for incorporating and maintaining reliability and validity from external reviewers’ judgments to the investigators themselves. There have different opinions on validity with some suggesting that the concepts of validity is incompatible with qualitative research and should be abandoned while others argue efforts should be made to ensure validity so as to lend credibility to the results. This paper is an attempt to clarify the meaning and use of reliability and validity in the qualitative research paradigm.

  13. The Role of Conceptual Frameworks in Collecting Multisite Qualitative Data.

    Science.gov (United States)

    Lotto, Linda S.

    1983-01-01

    Examines the use of conceptual frameworks in collecting qualitative data from multiple sites. Presents strategies for devising frameworks that are flexible and general without sacrificing specificity. (JOW)

  14. Qualitative Psychology Nexus, Vol. X: Values and Diversity in Qualitative Research

    OpenAIRE

    Schweizer, Karin; Christel, Taylor; Ullrich, Anette; Huber, Günter L.; Lim, Levan; von Lautz, Alex; Estalella, Adolfo; Suorsa, Teemu; Lambert, Richard G.; McCarthy, Christopher; Zimber, Andreas; Comtesse, Hannah; Flavian, Heidi; Tussia-Cohen, Betty; Kröner, Stephan

    2012-01-01

    Presentations from the annual workshops of the Center for Qualitative psychology in Sassary (Italy) in 2010 and in Heidelberg (Germany) in 2011 have been combined in this volume. The book has twelve chapters, organized in two parts: Reflections on Values and Diversity (Part I), Methodological Impacts (Part II). All chapters are related to a specific aspect of diversity and have been presented at either one of the workshops in Italy or in Germany. The term "diversity" is often used to descr...

  15. Non-separation assay for glycohemoglobin.

    Science.gov (United States)

    Blincko, S; Edwards, R

    1998-06-01

    The determination of glycohemoglobin [HbA1c, HbA1, or total glycohemoglobin (GHb)] has become an established procedure in the management of diabetes mellitus. Here, we describe the development of a simple, fluorescence, non-separation assay for the percentage of GHb (%GHb). The fluorescence of an eosin-boronic acid derivative when it was mixed with hemolysates of unwashed erythrocytes was quenched in proportion to the percentage of glycohemoglobin. Measurement of the fluorescence intensity gave an estimate of GHb in the sample, and measurement of light absorbance gave an estimate of total hemoglobin. A combination of the two measurements gave the assay response. Comparison with HPLC (Menarini-Arkray HA-8140 fully automated analyzer) for the percentage of HbA1 (%HbA1) gave %GHb(NETRIA) = 1.1(SD +/-0.03)%HbA1 +0.6(SD +/-0.3), S(y/x) = 0.821, r = 0.972, n = 80; comparison for HbA1c gave %GHb(NETRIA) = 1.3(SD +/-0.04)%HbA1c + 1.8(SD +/-0.3), S(y/x) = 0.813, r = 0.973, n = 80. Precision, estimated as the percentage of the CV of the %GHb assay results, was <2% (intraassay, range 5-22% GHb) and <4.2% (interassay, range 4-16% GHb). Dilution of a high-percentage GHb sample lysate showed that the assay was linear, and addition of glucose (60 mmol/L), bilirubin (250 micromol/L), and triglycerides (14 mmol/L) to low, medium, and high %GHb samples showed no clinical interference in assay results. PMID:9625057

  16. Nondestructive waste assay capability evaluation project

    International Nuclear Information System (INIS)

    For most containerized waste forms, the most common and efficient means to determine the TRU material quantity is the use of nondestructive assay (NDA) techniques. Requirements and quality assurance objectives (QAOs) for NDA techniques employed to characterize TRU waste destined for the Waste Isolation Pilot Plant are delineated in the National TRU Program Transuranic Waste Characterization Quality Assurance Program Plan (QAPP). These QAOs represent minimum performance requirements consisting of allowable measurement parameter ranges such as bias and precision for which compliance must be demonstrated in accordance with the prescriptions of the QAPP. To establish actual capabilities of existing nondestructive waste assay systems relative to the requirements of the QAPP, a mechanism is required to acquire performance data for waste types typical of the DOE inventory and to objectively evaluate such data. In accordance with this need, the DOE mixed waste focus area operating in conjunction with the characterization monitoring and sensor technology crosscut area program has instituted an assay capability evaluation project (CEP). The project is focused on mobile NDA technologies but is applicable to most permanently installed systems operating the same NDA technology. The evaluation project is currently being conducted at the Idaho National Engineering and Environmental Laboratory (INEEL) Radioactive Waste Management Complex (RWMC) using actual Rocky Flats Plant-generated waste forms currently in storage and appropriate matrix/radioactive material surrogates. Six mobile waste assay system technologies are participating in the CEP: (1) segmented gamma scanner; (2) active and passive computed tomography; (3) high-efficiency neutron counter; (4) IQ3-gamma assay system; (5) tomographic gamma scanner; and (6) an imaging passive/active neutron system

  17. Assays for mammalian tyrosinase: a comparative study

    International Nuclear Information System (INIS)

    This work describes a comparative study of the tyrosinase activity determined using three methods which are the most extensively employed; two radiometric assays using L-tyrosine as substrate (tyrosine hydroxylase and melanin formation activities) and one spectrophotometric assay using L-dopa (dopa oxidase activity). The three methods were simultaneously employed to measure the activities of the soluble, melanosomal, and microsomal tyrosinase isozymes from Harding-Passey mouse melanoma through their purification processes. The aim of this study was to find any correlation among the tyrosinase activities measured by the three different assays and to determine whether that correlation varied with the isozyme and its degree of purification. The results show that mammalian tyrosinase has a greater turnover number for L-dopa than for L-tyrosine. Thus, enzyme activity, expressed as mumol of substrate transformed per min, is higher in assays using L-dopa as substrate than those using L-tyrosine. Moreover, the percentage of hydroxylated L-tyrosine that is converted into melanin is low and is affected by several factors, apparently decreasing the tyrosinase activity measured by the melanin formation assay. Bearing these considerations in mind, average interassay factors are proposed. Their values are 10 to transform melanin formation into tyrosine hydroxylase activity, 100 to transform tyrosine hydroxylase into dopa oxidase activity, and 1,000 to transform melanin formation into dopa oxidase activity. Variations in these values due to the presence in the tyrosinase preparations of either inhibitors or regulatory factors in melanogenesis independent of tyrosinase are also discussed

  18. Production and assay of forskolin antibodies

    International Nuclear Information System (INIS)

    Forskolin (Fo), a cardiovascular active diterpene of plant origin, has been widely used as a research tool in regulation of the catalytic activity of adenylate cyclase (AC). A linear relationship of Fo binding to plasma membrane with activation of AC has been reported. The present abstract describes the production and assay of Fo antibodies (AB). 7-0-Hemisuccinyl-7-deacetyl Fo, coupled to either human serum albumin or goat IgG, was injected into goats to elicit AB to Fo haptan. AB to Fo in antiserum or an isolated IgG fraction was tested by two assay methods, a radioimmunoassay using 3H-Fo as a tracer and a colorimetric enzyme-linked immunosorbent assay (ELISA) using horse radish peroxidase-rabbit anti goat IgG as indicator. The titers for Fo antiserum were 4000-10,000. In the defined assay condition, approximately 20-25% of the added 3H-Fo was found to bind to AB. The bound radioactivity was displaced by Fo-HSA or Fo-goat IgG or free unlabelled Fo ranging from 0.5-50 pmol/tube, or 5-500 nM. The IC50 was approximately 8-10 pmol/tube or 80-100 nM. The binding of HRP-rabbit anti goat IgG in the ELISA was inhibited by proper Fo conjugate. The development of methods for production and assay for Fo AB may be useful in the study of mechanism of activation of AC by Fo and Fo-like compound

  19. 21 CFR 864.7100 - Red blood cell enzyme assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Red blood cell enzyme assay. 864.7100 Section 864... enzyme assay. (a) Identification. Red blood cell enzyme assay is a device used to measure the activity in... kinase or 2,3-diphosphoglycerate. A red blood cell enzyme assay is used to determine the enzyme...

  20. 21 CFR 866.3305 - Herpes simplex virus serological assays.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Herpes simplex virus serological assays. 866.3305... simplex virus serological assays. (a) Identification. Herpes simplex virus serological assays are devices... herpes simplex virus in serum. Additionally, some of the assays consist of herpes simplex virus...

  1. 21 CFR 864.7375 - Glutathione reductase assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Glutathione reductase assay. 864.7375 Section 864... reductase assay. (a) Identification. A glutathione reductase assay is a device used to determine the... fluorescence and photometry. The results of this assay are used in the diagnosis of liver disease,...

  2. 21 CFR 864.7455 - Fetal hemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Fetal hemoglobin assay. 864.7455 Section 864.7455... assay. (a) Identification. A fetal hemoglobin assay is a device that is used to determine the presence... hemoglobin present. The assay may be used to detect fetal red cells in the maternal circulation or to...

  3. 21 CFR 866.3225 - Enterovirus nucleic acid assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Enterovirus nucleic acid assay. 866.3225 Section... nucleic acid assay. (a) Identification. An enterovirus nucleic acid assay is a device that consists of... Special Controls Guidance Document: Nucleic Acid Amplification Assay for the Detection of Enterovirus...

  4. Evaluierung eines Qualitäts- und Herkunftszeichens: Das Beispiel "Geprüfte Qualität - HESSEN"

    OpenAIRE

    Will, Sabine

    2013-01-01

    Die Dissertation führt eine umfassende Erfolgskontrolle des Qualitäts- und Herkunftszeichens „Geprüfte Qualität – HESSEN“ (GQH) aus Sicht der verschiedenen Akteure durch. Die GQH ist ein regionales Qualitätssicherungssystem des hessischen Agrarmarketings. Da die Wirtschaftspartner zunehmend finanziell beteiligt sind, steigt die Erfolgsabhängigkeit des Marketings und Erfolgsanalysen sind von zunehmender Bedeutung. Auf Verbraucherebene erfolgt daher eine produktübergreifende Bewertung der Marke...

  5. Qualität ist kein Zufall. Einblick in die Qualitätsentwicklung der Wiener Volkshochschulen

    OpenAIRE

    Pig, Christine; Schiller, Renate

    2011-01-01

    Der vorliegende Beitrag beschreibt die Maßnahmen und Verfahren, die die Wiener Volkshochschulen GmbH einsetzt, um auf Basis einer kontinuierlichen Qualitätssicherung, eines abgestimmten Bildungsangebots und eines qualitätsvollen Unterrichts gelungenes Lernen, gelungene Beratung und eine gelungene Dienstleistung zu ermöglichen. Für den derzeit laufenden Qualitätsentwicklungsprozess sind drei Instrumente maßgeblich: das Weißbuch Programmplanung, das auf die Weiterentwicklung des Programmangebot...

  6. A quantitative comet infection assay for influenza virus

    OpenAIRE

    Lindsay, Stephen M.; Timm, Andrea; Yin, John

    2011-01-01

    The virus comet assay is a cell-based virulence assay used to evaluate an antiviral drug or antibody against a target virus. The comet assay differs from the plaque assay in allowing spontaneous flows in 6-well plates to spread virus. When implemented quantitatively the comet assay has been shown to have an order-of-magnitude greater sensitivity to antivirals than the plaque assay. In this study, a quantitative comet assay for influenza virus is demonstrated, and is shown to have a 13-fold in...

  7. Comparative titers of egg assay against immunofluorescent assay of Chlamydia psittaci.

    OpenAIRE

    Tessler, J

    1985-01-01

    A comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in Vero cells with and without cortisone plus cytochalasin B. The titer of NJ-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin B and cortisone. The SCT and CDC strains had approximately the same titers in the egg titration and the fluorescent antibody a...

  8. Qualitative research: adding drive and dimension to clinical research.

    Science.gov (United States)

    Rusinová, Katerĭna; Pochard, Frédéric; Kentish-Barnes, Nancy; Chaize, Marine; Azoulay, Elie

    2009-01-01

    Qualitative research and its methods stem from the social sciences and can be used to describe and interpret complex phenomena that involve individuals' views, beliefs, preferences, and subjective responses to places and people. Thus, qualitative research explores the many subjective factors that may influence patient outcomes, staff well-being, and healthcare quality, yet fail to lend themselves to the hypothesis-testing approach that characterizes quantitative research. Qualitative research is valuable in the intensive care unit to explore organizational and cultural issues and to gain insight into social interactions, healthcare delivery processes, and communication. Qualitative research generates explanatory models and theories, which can then serve to devise interventions, whose efficacy can be studied quantitatively. Thus, qualitative research works synergistically with quantitative research, providing new impetus to the research process and a new dimension to research findings. Qualitative research starts with conceptualizing the research question, choosing the appropriate qualitative strategy, and designing the study; rigorous methods specifically designed for qualitative research are then used to conduct the study, analyze the data, and verify the findings. The researcher is the data-collecting instrument, and the data are the participants' words and behaviors. Data coding methods are used to describe experiences, discover themes, and build theories. In this review, we outline the rationale and methods for conducting qualitative research to inform critical care issues. We provide an overview of available qualitative methods and explain how they can work in close synergy with quantitative methods. To illustrate the effectiveness of combining different research methods, we will refer to recent qualitative studies conducted in the intensive care unit. PMID:19104214

  9. Murder and Motivation: A Qualitative Study

    Directory of Open Access Journals (Sweden)

    Mirzaei Alavijeh

    2015-05-01

    Full Text Available Background Murder is considered as the worst crime in all societies. It might be accomplished suddenly based on previous resolution, effect of stimuli, or due to a mental condition. Objectives The present study aimed to determine the reasons and motivations to commit a murder from murderers’ point of view. Patients and Methods It is a qualitative research making use of content analysis, conducting on 17 male imprisoned murderers. Participants were selected through goal based sampling to gain data saturation among prisoners in central prison of Kermanshah. Deep individual and semi-structured interviews were conducted using general questions designed as interview guide. In the meantime, personal satisfaction, information privacy, liberty to leave the study, and moral commitment of the interviews were observed. Results In the end, five main contents were found: collective quarrel, honor killing, emotional dissatisfaction, lack of control on anger, and burglary. Conclusions It seems that prevention of collective quarrel and control on anger could be helpful to decrease the murder rate in society.

  10. Peer Education: Participatory Qualitative Educational Needs Assessment.

    Directory of Open Access Journals (Sweden)

    Shirin Djalalinia

    2013-12-01

    Full Text Available In the area of youth health, peers education is an approach to health promotion. Assess the training needs of peers educators clarifies the components, values, and quality of training protocols. Aim to that we conducted a participatory educational needs assessment of youth peer educators.Involving youth and key informants in direct collaboration with research team, a qualitative approach was planned based on grounded theory. For data collection a semi-structured guide questioning was designed. Sixteen focus group discussions and 8 in depth interview were held.The majority of participants emphasized on the importance of mental health, life skills, AIDS prevention, contraception methods, and healthy nutrition as the main training topics. They were extremely interested into the comprehensive educational material among their participatory role in peer programs.The training programs should be well defined based on the knowledge, skills and behavior of peers. During the implementation, training programs should be followed to meet the ongoing educational needs of service providers.

  11. QUALITATIVE DRUG: WHAT SHOULD IT LOOK LIKE?

    Directory of Open Access Journals (Sweden)

    V. V. Iakusevich

    2016-01-01

    Full Text Available Drug quality in modern market is discussed. There is an increase of a number of drugs that don’t meet to the quality requirements.Most of them are produced by Russian manufacturers, which number has also increased recently. According to official data 6-10%of all medicines are counterfeit. Quality reduction is also connected with a huge number (80% of reproduced drugs (generics. Considerable part among generics doesn’t pass required evaluation of equivalence with original product. Approved deviations (according to Russian regulative rules in pharmacological equivalence may reach 5%, and in pharmacokinetic equivalence -25%. Evidences of pharmacodynamic (therapeutic equivalence are not required at all. At the same time, author advocates the production of qualitative generics that can providemodern and accessible treatment. Generic quality should be confirmed by randomized studies with cross-over designed comparison with original drug in parallel groups. The examples of the generics with such evidences are demonstrated. The requirements to the data about drugs are given to help the physician to make a right independent estimation of its quality.

  12. Validation of qualitative microbiological test methods.

    Science.gov (United States)

    IJzerman-Boon, Pieta C; van den Heuvel, Edwin R

    2015-01-01

    This paper considers a statistical model for the detection mechanism of qualitative microbiological test methods with a parameter for the detection proportion (the probability to detect a single organism) and a parameter for the false positive rate. It is demonstrated that the detection proportion and the bacterial density cannot be estimated separately, not even in a multiple dilution experiment. Only the product can be estimated, changing the interpretation of the most probable number estimator. The asymptotic power of the likelihood ratio statistic for comparing an alternative method with the compendial method, is optimal for a single dilution experiment. The bacterial density should either be close to two CFUs per test unit or equal to zero, depending on differences in the model parameters between the two test methods. The proposed strategy for method validation is to use these two dilutions and test for differences in the two model parameters, addressing the validation parameters specificity and accuracy. Robustness of these two parameters might still be required, but all other validation parameters can be omitted. A confidence interval-based approach for the ratio of the detection proportions for the two methods is recommended, since it is most informative and close to the power of the likelihood ratio test. PMID:25412584

  13. Experiences of Kratom Users: A Qualitative Analysis.

    Science.gov (United States)

    Swogger, Marc T; Hart, Elaine; Erowid, Fire; Erowid, Earth; Trabold, Nicole; Yee, Kaila; Parkhurst, Kimberly A; Priddy, Brittany M; Walsh, Zach

    2015-01-01

    Kratom (Mitragyna speciosa) is a psychoactive plant that has been used since at least 1836 in folk medicine in Southeast Asian countries. More recently, kratom has become widely available in the West and is used for both recreational and medicinal purposes. There has, however, been little scientific research into the short- and long-term effects of kratom in humans, and much of the information available is anecdotal. To supplement the increasing scientific understanding of kratom's pharmacology and research into its effects in animals, we report the results of a qualitative analysis of first-hand descriptions of human kratom use that were submitted to, and published by, a psychoactive substance information website (Erowid.org). Themes that emerged from these experience reports indicate that kratom may be useful for analgesia, mood elevation, anxiety reduction, and may aid opioid withdrawal management. Negative response themes also emerged, indicating potential problems and unfavorable "side" effects, especially stomach upset and vomiting. Based on our analyses, we present preliminary hypotheses for future examination in controlled, quantitative studies of kratom. PMID:26595229

  14. Diagnostic reasoning using qualitative causal models

    International Nuclear Information System (INIS)

    The application of expert systems to reasoning problems involving real-time data from plant measurements has been a topic of much research, but few practical systems have been deployed. One obstacle to wider use of expert systems in applications involving real-time data is the lack of adequate knowledge representation methodologies for dynamic processes. Knowledge bases composed mainly of rules have disadvantages when applied to dynamic processes and real-time data. This paper describes a methodology for the development of qualitative causal models that can be used as knowledge bases for reasoning about process dynamic behavior. These models provide a systematic method for knowledge base construction, considerably reducing the engineering effort required. They also offer much better opportunities for verification and validation of the knowledge base, thus increasing the possibility of the application of expert systems to reasoning about mission critical systems. Starting with the Signed Directed Graph (SDG) method that has been successfully applied to describe the behavior of diverse dynamic processes, the paper shows how certain non-physical behaviors that result from abstraction may be eliminated by applying causal constraint to the models. The resulting Extended Signed Directed Graph (ESDG) may then be compiled to produce a model for use in process fault diagnosis. This model based reasoning methodology is used in the MOBIAS system being developed by Duke Power Company under EPRI sponsorship. 15 refs., 4 figs

  15. Stigma in Cirrhotic Patients: A Qualitative Study.

    Science.gov (United States)

    Shabanloei, Reza; Ebrahimi, Hossein; Ahmadi, Fazlollah; Mohammadi, Eesa; Dolatkhah, Roya

    2016-01-01

    Stigma is one of the main problems of patients suffering from cirrhosis, and it causes many challenges for the patients and their treatment. The present study aimed to discover and define the perceived stigma by cirrhotic patients. This qualitative study was conducted through a content analysis approach. The participants were 15 patients suffering from cirrhosis. Data were collected via semistructured, in-depth interviews and analyzed on the basis of methods described by Granheme and Landman. During data analysis, stigma was categorized into four categories and 13 subcategories: external representation of social stigma (others' avoidance behaviors, inadmissible tag, discriminative behaviors of treatment personnel, blaming behaviors), internal representation of social stigma (social ostracism, social isolation, curiosity to perceive people's perceptions), external representation of self-stigma (fear of disclosure of illness, threatening situation, difficult emotional relationships), and internal representation of self-stigma (condemned to suffer, self-punishment, self-alienation). Experiencing stigma is common among cirrhotic patients and may affect patients' coping with the illness and treatment. Thus, it is specifically important that treatment personnel know patients' perception, provide comprehensive support for these patients, and plan to enhance public awareness about the disease recommended. PMID:27258462

  16. Quantitative and qualitative coronary arteriography. 1

    International Nuclear Information System (INIS)

    The clinical objectives of arteriography are to obtain information that contributes to an understanding of the mechanisms of the clinical syndrome, provides prognostic information, facilitates therapeutic decisions, and guides invasive therapy. Quantitative and improved qualitative assessments of arterial disease provide us with a common descriptive language which has the potential to accomplish these objectives more effectively and thus to improve clinical outcome. In certain situations, this potential has been demonstrated. Clinical investigation using quantitative techniques has definitely contributed to our understanding of disease mechanisms and of atherosclerosis progression/regression. Routine quantitation of clinical images should permit more accurate and repeatable estimates of disease severity and promises to provide useful estimates of coronary flow reserve. But routine clinical QCA awaits more cost- and time-efficient methods and clear proof of a clinical advantage. Careful inspection of highly magnified, high-resolution arteriographic images reveals morphologic features related to the pathophysiology of the clinical syndrome and to the likelihood of future progression or regression of obstruction. Features that have been found useful include thrombus in its various forms, ulceration and irregularity, eccentricity, flexing and dissection. The description of such high-resolution features should be included among, rather than excluded from, the goals of image processing, since they contribute substantially to the understanding and treatment of the clinical syndrome. (author). 81 refs.; 8 figs.; 1 tab

  17. Qualitative and Asymptotic Theory of Detonations

    KAUST Repository

    Faria, Luiz

    2014-11-09

    Shock waves in reactive media possess very rich dynamics: from formation of cells in multiple dimensions to oscillating shock fronts in one-dimension. Because of the extreme complexity of the equations of combustion theory, most of the current understanding of unstable detonation waves relies on extensive numerical simulations of the reactive compressible Euler/Navier-Stokes equations. Attempts at a simplified theory have been made in the past, most of which are very successful in describing steady detonation waves. In this work we focus on obtaining simplified theories capable of capturing not only the steady, but also the unsteady behavior of detonation waves. The first part of this thesis is focused on qualitative theories of detonation, where ad hoc models are proposed and analyzed. We show that equations as simple as a forced Burgers equation can capture most of the complex phenomena observed in detonations. In the second part of this thesis we focus on rational theories, and derive a weakly nonlinear model of multi-dimensional detonations. We also show, by analysis and numerical simulations, that the asymptotic equations provide good quantitative predictions.

  18. Qualitative Analysis for Maintenance Process Assessment

    Science.gov (United States)

    Brand, Lionel; Kim, Yong-Mi; Melo, Walcelio; Seaman, Carolyn; Basili, Victor

    1996-01-01

    In order to improve software maintenance processes, we first need to be able to characterize and assess them. These tasks must be performed in depth and with objectivity since the problems are complex. One approach is to set up a measurement-based software process improvement program specifically aimed at maintenance. However, establishing a measurement program requires that one understands the problems to be addressed by the measurement program and is able to characterize the maintenance environment and processes in order to collect suitable and cost-effective data. Also, enacting such a program and getting usable data sets takes time. A short term substitute is therefore needed. We propose in this paper a characterization process aimed specifically at maintenance and based on a general qualitative analysis methodology. This process is rigorously defined in order to be repeatable and usable by people who are not acquainted with such analysis procedures. A basic feature of our approach is that actual implemented software changes are analyzed in order to understand the flaws in the maintenance process. Guidelines are provided and a case study is shown that demonstrates the usefulness of the approach.

  19. Experiencia in qualitative methodology: History of life

    Directory of Open Access Journals (Sweden)

    María del Socorro Borboa Quintero

    2012-05-01

    Full Text Available From the commercial opening of Mexico the companies compete with transnational companies. In the municipality of Culiacan of the state of Sinaloa the small companies have been harmed mainly by his low level of productivity and competitiveness. Reason why to study two people who even with the complexity of the environment they haveinfluenced for the growth and development of its organization which is important for the local and state development. The intention of the present work, even though some results are included, is to show the method and the instruments used in the investigation. In the first place one poses the problem to investigate, the questions, objectives and hypotheses that orient this work, also includes the design of the field work, that contemplates relevance to use the qualitative method of the tradition life history, the collect and the storage of the data, its analysis and interpretation and some conclusions. The study allowed determining the characteristics and qualities that conform their personality of leader, as well as the identification of the management practices that have implemented.

  20. Heliconia phenotypic diversity based on qualitative descriptors.

    Science.gov (United States)

    Guimarães, W N R; Martins, L S S; Castro, C E F; Carvalho Filho, J L S; Loges, V

    2014-01-01

    The aim of this study was to characterize Heliconia genotypes phenotypically using 26 qualitative descriptors. The evaluations were conducted in five flowering stems per clump in three replicates of 22 Heliconia genotypes. Data were subjected to multivariate analysis, the Mahalanobis dissimilarity measure was estimated, and the dendrogram was generated using the nearest neighbor method. From the values generated by the dissimilarity matrix and the clusters formed among the Heliconia genotypes studied, the phenotypic characterizations that best differentiated the genotypes were: pseudostem and wax green tone (light or dark green), leaf-wax petiole, the petiole hair, cleft margin at the base of the petiole, midrib underside shade of green, wax midrib underside, color sheet (light or dark green), unequal lamina base, torn limb, inflorescence-wax, position of inflorescence, bract leaf in apex, twisting of the rachis, and type of bloom. These results will be applied in the preparation of a catalog for Heliconia descriptors, in the selection of different genotypes with most promising characteristics for crosses, and for the characterization of new genotypes to be introduced in germplasm collections. PMID:24782170

  1. Sexual activity during menstruation: a qualitative study.

    Science.gov (United States)

    Allen, Katherine R; Goldberg, Abbie E

    2009-01-01

    This study utilized a grounded theory method to analyze qualitative narratives about sexuality and menstruation from 108 young women (ages 18-23; M = 19.8, SD = 1.07) and 12 young men (ages 18-24; M = 20.4, SD = 1.46). Five patterns were found: Sixteen women identified themselves as virgins and had not faced the issue of negotiating sex during menstruation. Among the 92 women who said they were sexually active, 37 women said they would never have intercourse during menstruation, eight women said they tried it once but never would again, and seven women said they rarely would and only under certain conditions. The largest group, 40 women, said they do have menstrual sex. Compared to the other groups, more of the women who do have sex during menstruation were in committed relationships, and none espoused a discourse of disgust. Considering the 12 men, three were virgins. Among the nine sexually experienced men, seven said they did have sex with a menstruating partner. Young adults who were comfortable with menstrual sex saw it as just another part of a committed intimate relationship. PMID:19350441

  2. Is the Comet Assay a Sensitive Procedure for Detecting Genotoxicity?

    OpenAIRE

    Sasaki, Yu F.; Satomi Kawaguchi; Takanori Nakamura; Gisho Honda; Ayumi Yamamoto

    2010-01-01

    Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine whether the power of the Comet assay to detect a low level of genotoxic potential is superior to those of other genotoxicity assays in mammalian cells, we compared the results of Comet assay with those of micronucleus test (MN test). WTK1 hum...

  3. Using Blogs in Qualitative Educational Research: An Exploration of Method

    Science.gov (United States)

    Harricharan, Michelle; Bhopal, Kalwant

    2014-01-01

    When compared with wider social research, qualitative educational research has been relatively slow to take up online research methods (ORMs). There is some very notable research in the area but, in general, ORMs have not achieved wide applicability in qualitative educational contexts apart from research that is inherently linked to the Internet,…

  4. Solicited diary studies of psychotherapy in qualitative research

    DEFF Research Database (Denmark)

    Mackrill, Thomas Edward

    2008-01-01

    Diary studies are scarce within the field of qualitative psychotherapy research. In this article arguments for and against the employment of solicited diaries studies in qualitative psychotherapy research are investigated. The strengths of diary studies are presented along with arguments concerning...

  5. Incorporating Translation in Qualitative Studies: Two Case Studies in Education

    Science.gov (United States)

    Sutrisno, Agustian; Nguyen, Nga Thanh; Tangen, Donna

    2014-01-01

    Cross-language qualitative research in education continues to increase. However, there has been inadequate discussion in the literature concerning the translation process that ensures research trustworthiness applicable for bilingual researchers. Informed by the literature on evaluation criteria for qualitative data translation, this paper…

  6. Visual Methodology in Classroom Inquiry: Enhancing Complementary Qualitative Research Designs

    Science.gov (United States)

    Kingsley, Joanne

    2009-01-01

    This article presents the argument that combining visual methods with other qualitative research methods enhances the inherent strengths of each methodology and allows new understandings to emerge. These would otherwise remain hidden if only one method were used in isolation. In a qualitative inquiry of an elementary teacher's constructivist…

  7. Conducting Qualitative Research on Desertification in Western Lesvos, Greece

    Science.gov (United States)

    Iosifides, Theodoros; Politidis, Theodoros

    2005-01-01

    The main aim of this article is to present some critical methodological strategies employed in a qualitative research study on local socioeconomic development and desertification in western Lesvos, Greece. Through in-depth qualitative interviews with local producers in western Lesvos, Greece, an effort was made to identify and analyze the links…

  8. Beyond Constant Comparison Qualitative Data Analysis: Using NVivo

    Science.gov (United States)

    Leech, Nancy L.; Onwuegbuzie, Anthony J.

    2011-01-01

    The purposes of this paper are to outline seven types of qualitative data analysis techniques, to present step-by-step guidance for conducting these analyses via a computer-assisted qualitative data analysis software program (i.e., NVivo9), and to present screenshots of the data analysis process. Specifically, the following seven analyses are…

  9. “Slow Food” Post-Qualitative Research in Psychology

    DEFF Research Database (Denmark)

    Demuth, Carolin

    2015-01-01

    The present paper addresses several aspects discussed in the special issue on the future of qualitative research in psychology. Particularly, it asks whether in light of the overhomogenization of the term “qualitative methods” researchers actually can still assume that they talk about the same...

  10. Contributions of Qualitative Research to the Validity of Intervention Research

    Science.gov (United States)

    Nastasi, B.K.; Schensul, S.L.

    2005-01-01

    The Interdisciplinary Qualitative Research Subcommittee (IQRS) of the Task for on Evidence-based Interventions in School Psychology assumed the responsibility of developing criteria for reviewing qualitative methods used in intervention research. The IQRS, composed primarily of psychologists and anthropologists, was confronted with the…

  11. Quality and Trustworthiness in Qualitative Research in Counseling Psychology.

    Science.gov (United States)

    Morrow, Susan L.

    2005-01-01

    This article examines concepts of the trustworthiness, or credibility, of qualitative research. Following a "researcher-as-instrument," or self-reflective, statement, the paradigmatic underpinnings of various criteria for judging the quality of qualitative research are explored, setting the stage for a discussion of more transcendent standards…

  12. Communicating Qualitative Research Study Designs to Research Ethics Review Boards

    Science.gov (United States)

    Ells, Carolyn

    2011-01-01

    Researchers using qualitative methodologies appear to be particularly prone to having their study designs called into question by research ethics or funding agency review committees. In this paper, the author considers the issue of communicating qualitative research study designs in the context of institutional research ethics review and offers…

  13. Exploring School Counselors' Perceptions of Vicarious Trauma: A Qualitative Study

    Science.gov (United States)

    Parker, Mashone; Henfield, Malik S.

    2012-01-01

    The purpose of this qualitative study was to examine school counselors' perceptions of vicarious trauma. Consensual qualitative research (CQR) methodology was used. Six school counselors were interviewed. Three primary domains emerged from the data: (a) ambiguous vicarious trauma, (b) support system significance, and (c) importance of level of…

  14. Blending Qualitative & Quantitative Research Methods in Theses and Dissertations.

    Science.gov (United States)

    Thomas, R. Murray

    This guide discusses combining qualitative and quantitative research methods in theses and dissertations. It covers a wide array of methods, the strengths and limitations of each, and how they can be effectively interwoven into various research designs. The first chapter is "The Qualitative and the Quantitative." Part 1, "A Catalogue of…

  15. Interviewing Objects: Including Educational Technologies as Qualitative Research Participants

    Science.gov (United States)

    Adams, Catherine A.; Thompson, Terrie Lynn

    2011-01-01

    This article argues the importance of including significant technologies-in-use as key qualitative research participants when studying today's digitally enhanced learning environments. We gather a set of eight heuristics to assist qualitative researchers in "interviewing" technologies-in-use (or other relevant objects), drawing on concrete…

  16. Caring for an Individual with Autism Disorder: A Qualitative Analysis

    Science.gov (United States)

    Phelps, Kenneth W.; Hodgson, Jennifer L.; McCammon, Susan L.; Lamson, Angela L.

    2009-01-01

    Background: Caregivers in this qualitative study reported the multidimensional implications of having a child with autism on their family's lives and overall functioning. Method: The Effects of the Situation Questionnaire (Yatchmenoff, Koren, Friesen, Gordon, & Kinney, 1998) was used to gather qualitative data from 80 caregivers. Colaizzi's (1978)…

  17. Polish Qualitative Sociology. Insight into the future of postdisciplinary research

    OpenAIRE

    Konecki, Krzysztof

    2014-01-01

    The paper desctibes the definitions of following concepts: multidisiplinarity, interdisciplinarity, transdysciplinarity, postdisciplinarity. MOreover it discuss the meanings of a concept of discipline. It describes the place of the Polish qualitative sociology in the context of postdisciplinary research. The main question of paper is: Does the POlish Qualitative Sociology has entered the postdisciplinary phase of research? DGS, UL Krzysztof Konecki

  18. Ethical Perspectives on Qualitative Research in Applied Psychology

    Science.gov (United States)

    Haverkamp, Beth E.

    2005-01-01

    The present article explores ethical issues that emerge in qualitative research conducted by applied psychologists. The utility and relevance of the Ethical Principles of Psychologists and Code of Conduct (American Psychological Association, 2002) for qualitative research are examined. The importance of psychology's fiduciary relationship with…

  19. Qualitative vs. quantiative research on FL teaching and learning process

    OpenAIRE

    Komorowska, Hanna

    1990-01-01

    Part 1 of the article contains a discussion of quantitative research projects described as structuralistic, deductive, statistical and objective. Main lines of criticism directed against quantitative research projects are also presented. Part 2 contains characteristic of qualitative research projects described as holistic, inductive, descriptive and subjective. Theoretical affiliation of qualitative research projects to ethnometh-odology, ethnography, phenomenology is also analyzed. P...

  20. A Critical Review of Qualitative Interviews in Applied Linguistics

    Science.gov (United States)

    Mann, Steve

    2011-01-01

    This article asks what applied linguistics can learn from related disciplines with regard to the collection, analysis and representation of qualitative interviews. It assesses the contributions of qualitative sociology, anthropology, discursive psychology and outlines four "discourse dilemmas" which might provide the basis for a more critical and…

  1. A Qualitative Phenomenological Exploration of Teachers' Experience With Nutrition Education

    OpenAIRE

    Hall, Elisha; Chai, Weiwen; Albrecht, Julie A.

    2016-01-01

    Background: Nutrition education delivered by classroom teachers has become a popular intervention designed to combat childhood obesity. However, few qualitative studies have explored nutrition education with teachers Purpose: The purpose of this study was to explore how elementary teachers describe their experience with nutrition education. Methods: A qualitative phenomenological approach was used. Semistructured interviews, observations, and document analysis were conducted with 10 teachers ...

  2. Trends in Qualitative Research in Language Teaching since 2000

    Science.gov (United States)

    Richards, Keith

    2009-01-01

    This paper reviews developments in qualitative research in language teaching since the year 2000, focusing on its contributions to the field and identifying issues that emerge. Its aims are to identify those areas in language teaching where qualitative research has the greatest potential and indicate what needs to be done to further improve the…

  3. The Importance of Qualitative Research for Causal Explanation in Education

    Science.gov (United States)

    Maxwell, Joseph A.

    2012-01-01

    The concept of causation has long been controversial in qualitative research, and many qualitative researchers have rejected causal explanation as incompatible with an interpretivist or constructivist approach. This rejection conflates causation with the positivist "theory" of causation, and ignores an alternative understanding of causation,…

  4. Estranged Familiars: A Deweyan Approach to Philosophy and Qualitative Research

    Science.gov (United States)

    Shuffelton, Amy

    2015-01-01

    This essay argues that philosophy can be combined with qualitative research without sacrificing the aims of either approach. Philosophers and qualitative researchers have articulated and supported the idea that human meaning-constructions are appropriately grasped through close attention to "consequences incurred in action," in…

  5. Somatic Sensitivity and Reflexivity as Validity Tools in Qualitative Research

    Science.gov (United States)

    Green, Jill

    2015-01-01

    Validity is a key concept in qualitative educational research. Yet, it is often not addressed in methodological writing about dance. This essay explores validity in a postmodern world of diverse approaches to scholarship, by looking at the changing face of validity in educational qualitative research and at how new understandings of the concept…

  6. Learning the Concept of Researcher as Instrument in Qualitative Research

    Science.gov (United States)

    Xu, Mengxuan Annie; Storr, Gail Blair

    2012-01-01

    The authors describe the process whereby a student with a background in economics was guided to understand the central role in qualitative research of the researcher as instrument. The instructor designed a three-part mock research project designed to provide experiential knowledge of the enterprise of qualitative research. Students, as neophyte…

  7. How People Interpret Healthy Eating: Contributions of Qualitative Research

    Science.gov (United States)

    Bisogni, Carole A.; Jastran, Margaret; Seligson, Marc; Thompson, Alyssa

    2012-01-01

    Objective: To identify how qualitative research has contributed to understanding the ways people in developed countries interpret healthy eating. Design: Bibliographic database searches identified reports of qualitative, empirical studies published in English, peer-reviewed journals since 1995. Data Analysis: Authors coded, discussed, recoded, and…

  8. Array-in-well platform-based multiplex assay for the simultaneous detection of anti-HIV- and treponemal-antibodies, and Hepatitis B surface antigen.

    Science.gov (United States)

    Talha, Sheikh M; Saviranta, Petri; Hattara, Liisa; Vuorinen, Tytti; Hytönen, Jukka; Khanna, Navin; Pettersson, Kim

    2016-02-01

    Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential of microarrays with the ease of handling microtiter wells. We have developed a single-step AIW assay for the simultaneous screening of HIV, Treponema pallidum subspecies pallidum (causing syphilis) and Hepatitis B virus infections targeting the specific detection of anti-HIV- and treponemal-antibodies and Hepatitis B surface antigen (HBsAg), respectively, using two different fluorescent label technologies i.e. DyLight 633 and europium nanoparticle. Double-antigen assay formats were used for anti-HIV- and treponemal-antibody detection that can simultaneously detect both IgG and IgM, and thus reduce the window period of detection. AIW assay was evaluated with well characterized serum/plasma samples (n=111), and the qualitative results were in near complete agreement with those of the reference assays. The AIW assay exhibited 100% sensitivities for all three analytes, and 100% specificities for anti-HIV antibodies and HBsAg, and 98.6% specificity for treponemal antibodies. The limit of detection of HBsAg in AIW assay was 0.18 ng/ml. This high performing AIW assay has the potential to be used as a multiplex screening test for these three infections. PMID:26711310

  9. Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.

    Science.gov (United States)

    Barbau-Piednoir, Elodie; Botteldoorn, Nadine; Yde, Marc; Mahillon, Jacques; Roosens, Nancy H

    2013-05-01

    A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) programme. The paper also proposes a new validation procedure to specifically validate qPCR assays applied to food microbiology according to two guidelines: the ISO 22118 norm and the "Definition of minimum performance requirements for analytical methods of GMO testing". The developed assays target the iap, prs and hlyA genes that belong to or neighbour the virulence cluster of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the SYBR®Green qPCR assays is two to five copies of target genes per qPCR reaction. These assays are highly accurate (98.08 and 100 % accuracy for the Listeria spp. and L. monocytogenes assays, respectively). PMID:23086339

  10. Event-specific qualitative and quantitative PCR detection of roundup ready event GT73 based on the 3'-integration junction.

    Science.gov (United States)

    Yang, Rong; Xu, Wentao; Luo, Yunbo; Guo, Feng; Lu, Yun; Huang, Kunlun

    2007-10-01

    With the development of genetically modified organisms, labeling regulations have been introduced, which require appropriate detection methods. Event-specific qualitative and quantitative polymerase chain reaction (PCR) detection methods have become the internationally agreed state-of-art. This paper describes an event-specific PCR method for qualitative and quantitative of Roundup Ready canola event GT73. The 3'-integration junction was characterized by two methods: inverse-PCR and thermal asymmetric interlaced-PCR. In the conventional qualitative PCR assay, the event-specific primers designed were confirmed to be specific and the limit of detection (LOD) was 0.05% (approximates to ten haploid genome copies). In the quantitative TaqMan real-time PCR assay, the LOD and the limit of quantification were five and ten haploid genome copies, respectively. In addition, for further quantitative detection, a reference molecule which contained the canola endogenous gene and event-specific sequence was constructed and standard curves were set up. The goodness of the linearity and high efficiency of the PCR reaction indicated the usability of the plasmid and the established PCR system. Moreover, mixed samples with different GT73 content (6, 3, 1 and 0.5%) were quantified using the established real-time PCR system to evaluate the trueness and precision of the system. The trueness expressed as bias varied from 2.00 to 18.00%. The precision expressed as variation coefficient were different from 6.40 to 32.95%. From above results, we believed that the established event-specific qualitative and quantitative PCR systems for GT73 in this study were acceptable and suitable for genetic modified canola detection. PMID:17554542

  11. Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies

    OpenAIRE

    Hongxia Lin; Susan Goodin; Strair, Roger K.; Robert S. DiPaola; Gounder, Murugesan K.

    2012-01-01

    Busulfan is used in preparative regimens for bone marrow transplantation and timely busulfan plasma concentration reporting is critical for subsequent dose adjustment. We compared two sensitive methods for pharmacokinetics studies including LC-MS assay and HPLC precolumn derivatization assay. Chromatographic separation was performed on a Gemini C18 column. Liquid-liquid extraction with ethyl acetate was used for plasma sample preparation. Busulfan and internal standard ([2H8]-busulfan) were d...

  12. Developments in plutonium waste assay at AWE.

    Science.gov (United States)

    Miller, T J

    2009-06-01

    In 2002 a paper was presented at the 43rd Annual Meeting of the Institute of Nuclear Materials Management (INMM) on the assay of low level plutonium (Pu) in soft drummed waste (Miller 2002 INMM Ann. Meeting (Orlando, FL, 23-27 July 2002)). The technique described enabled the Atomic Weapons Establishment (AWE), at Aldermaston in the UK, to meet the stringent Low Level Waste Repository at Drigg (LLWRD) conditions for acceptance for the first time. However, it was initially applied to only low density waste streams because it relied on measuring the relatively low energy (60 keV) photon yield from Am-241 during growth. This paper reviews the results achieved when using the technique to assay over 10,000 waste packages and presents the case for extending the range of application to denser waste streams. PMID:19454791

  13. Developments in plutonium waste assay at AWE

    Energy Technology Data Exchange (ETDEWEB)

    Miller, T J [AWE, Aldermaston, Reading, Berkshire RG7 4PR (United Kingdom)], E-mail: Timothy.Miller@awe.co.uk

    2009-06-15

    In 2002 a paper was presented at the 43rd Annual Meeting of the Institute of Nuclear Materials Management (INMM) on the assay of low level plutonium (Pu) in soft drummed waste (Miller 2002 INMM Ann. Meeting (Orlando, FL, 23-27 July 2002)). The technique described enabled the Atomic Weapons Establishment (AWE), at Aldermaston in the UK, to meet the stringent Low Level Waste Repository at Drigg (LLWRD) conditions for acceptance for the first time. However, it was initially applied to only low density waste streams because it relied on measuring the relatively low energy (60 keV) photon yield from Am-241 during growth. This paper reviews the results achieved when using the technique to assay over 10 000 waste packages and presents the case for extending the range of application to denser waste streams.

  14. High-Throughput Cell Toxicity Assays.

    Science.gov (United States)

    Murray, David; McWilliams, Lisa; Wigglesworth, Mark

    2016-01-01

    Understanding compound-driven cell toxicity is vitally important for all drug discovery approaches. With high-throughput screening (HTS) being the key strategy to find hit and lead compounds for drug discovery projects in the pharmaceutical industry [1], an understanding of the cell toxicity profile of hit molecules from HTS activities is fundamentally important. Recently, there has been a resurgence of interest in phenotypic drug discovery and these cell-based assays are now being run in HTS labs on ever increasing numbers of compounds. As the use of cell assays increases the ability to measure toxicity of compounds on a large scale becomes increasingly important to ensure that false hits are not progressed and that compounds do not carry forward a toxic liability that may cause them to fail at later stages of a project. Here we describe methods employed in the AstraZeneca HTS laboratory to carry out very large scale cell toxicity screening. PMID:27317000

  15. Expert system technology for nondestructive waste assay

    International Nuclear Information System (INIS)

    Nondestructive assay waste characterization data generated for use in the National TRU Program must be of known and demonstrable quality. Each measurement is required to receive an independent technical review by a qualified expert. An expert system prototype has been developed to automate waste NDA data review of a passive/active neutron drum counter system. The expert system is designed to yield a confidence rating regarding measurement validity. Expert system rules are derived from data in a process involving data clustering, fuzzy logic, and genetic algorithms. Expert system performance is assessed against confidence assignments elicited from waste NDA domain experts. Performance levels varied for the active, passive shielded, and passive system assay modes of the drum counter system, ranging from 78% to 94% correct classifications

  16. Mobile nondestructive assay and examination instruments

    International Nuclear Information System (INIS)

    A compact system that evaluates radioactive materials can furnish a big savings to taxpayers by ensuring that only properly identified nuclear waste is sent to a Department of Energy (DOE) radioactive waste storage area. The Los Alamos National Laboratory's Advanced Nuclear Technology Group has developed and field tested two easily transportable, self-contained modules: one x-rays the contents of special 208-iota shipment containers, the other assays the contents. The assay and evaluation system is a simple, portable solution to a complex problem that ensures that only properly packaged transuranic (TRU) waste is shipped to the Department of Energy's Waste Isolation Pilot Plant (WIPP). Caustic chemicals, liquids, and other objects or materials that could cause a container leak during shipment are the objects of an x-ray and video camera used in the system

  17. Nondestructive assay methods for solids containing plutonium

    International Nuclear Information System (INIS)

    Specific nondestructive assay (NDA) methods, e.g. calorimetry, coincidence neutron counting, singles neutron counting, and gamma ray spectrometry, were studied to provide the Savannah River Plant with an NDA method to measure the plutonium content of solid scrap (slag and crucible) generated in the JB-Line plutonium metal production process. Results indicate that calorimetry can be used to measure the plutonium content to within about 3% in 4 to 6 hours by using computerized equilibrium sample power predictive models. Calorimetry results confirm that a bias exists in the present indirect measurement method used to estimate the plutonium content of slag and crucible. Singles neutron counting of slag and crucible can measure plutonium to only +-30%, but coincidence neutron counting methods improve measurement precision to better than +-10% in less than ten minutes. Only four portions of a single slag and crucible sample were assayed, and further study is recommended

  18. System and method for assaying radiation

    Science.gov (United States)

    DiPrete, David P; Whiteside, Tad; Pak, Donald J; DiPrete, Cecilia C

    2013-11-12

    A system for assaying radiation includes a sample holder configured to hold a liquid scintillation solution. A photomultiplier receives light from the liquid scintillation solution and generates a signal reflective of the light. A control circuit biases the photomultiplier and receives the signal from the photomultiplier reflective of the light. A light impermeable casing surrounds the sample holder, photomultiplier, and control circuit. A method for assaying radiation includes placing a sample in a liquid scintillation solution, placing the liquid scintillation solution in a sample holder, and placing the sample holder inside a light impermeable casing. The method further includes positioning a photomultiplier inside the light impermeable casing and supplying power to a control circuit inside the light impermeable casing.

  19. Delayed gamma technique for fissile material assay

    Energy Technology Data Exchange (ETDEWEB)

    Mozin, Vladimir [Los Alamos National Laboratory; Tobin, Stephen [Los Alamos National Laboratory; Vujie, Jasmina [UC BERKELEY; Hunt, Alan [IDAHO ACCELERATOR CENTER

    2010-01-01

    Research sponsored by the Next Generation Safeguards Initiative are investigating several non-destructive assay techniques for the quantification of fissile plutonium mass in spent nuclear fuel assemblies. AppHcation of the delayed gamma signatures is investigated in this context. The objective of the research is to assess whether the delayed gamma assay instrument can provide sufficient sensitivity, isotope specificity and accuracy as required in nuclear material safeguards. This effort includes theoretical and experimental components for the optimal combination of interrogation parameters. A new modeling algorithm offering a high level of detail was developed specifically for this purpose and was validated in series of benchmark experiments. Preliminary modeling of the delayed gamma response from spent fuel assemblies was accomplished offering a future direction for the design process.

  20. Depleted uranium waste assay at AWE

    International Nuclear Information System (INIS)

    The Atomic Weapons Establishment (AWE) at Aldermaston has recently conducted a Best Practical Means (BPM) study, for solid Depleted Uranium (DU) waste assay, in order to satisfy key stakeholders that AWE is applying best practice. This study has identified portable passive High Resolution Gamma Spectrometry (HRGS), combined with an analytical software package called Spectral Nondestructive Assay Platform (SNAP), as the preferred option with the best balance between performance and costs. HRGS/SNAP performance has been assessed by monitoring 200 l DU waste drum standards and also heterogeneous, high density drums from DU firing trials. Accuracy was usually within 30 % with Detection Limits (DL) in the region of 10 g DU for short count times. Monte Carlo N-Particle (MCNP) calculations have been used to confirm the shape of the calibration curve generated by the SNAP software procured from Eberline Services Inc. (authors)