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Sample records for assay nf iso

  1. NF ISO 21243, September 2009. Radiation protection - Performance criteria for laboratories performing cytogenetic triage for assessment of mass casualties in radiological or nuclear emergencies - General principles and application to dicentric assay; NF ISO 21243, septembre 2009. Radioprotection - Criteres de performance pour les laboratoires pratiquant le tri par cytogenetique en cas d'accident radiologique ou nucleaire affectant un grand nombre de personnes - Principes generaux et application aux dicentriques

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-07-01

    This international standard is to give an overview of the minimum requirements of process and quality-control components of the cytogenetic response for triage of mass casualties. Cytogenetic triage is the use of chromosome damage to evaluate approximately and rapidly radiation doses received by individuals in order to supplement the early clinical categorization of casualties. This standard concentrates on organizational aspects of applying the dicentric assay for operation in a triage mode. The technical aspects of the dicentric assay can be found in the ISO 19238. This international standard is applicable either to an experienced biological dosimetry laboratory working alone or to a network of collaborating laboratories.

  2. ISO 17025 validation of a next-generation sequencing assay for relationship testing

    DEFF Research Database (Denmark)

    Buchard, Anders; Kampmann, Marie-Louise; Poulsen, Lena

    2016-01-01

    The HID-Ion AmpliSeq™ Identity Panel is a next-generation sequencing assay with 90 autosomal and 34 Y-chromosome SNPs that are amplified in one PCR step and subsequently sequenced using the Ion Personal Genome Machine (Ion PGM™) System. This assay was validated for relationship testing in our ISO...

  3. Modulating temporal control of NF-kappaB activation: implications for therapeutic and assay selection.

    Science.gov (United States)

    Klinke, David J; Ustyugova, Irina V; Brundage, Kathleen M; Barnett, John B

    2008-06-01

    The activation of transcription factor NF-kappaB (nuclear factor-kappaB) plays a central role in the induction of many inflammatory response genes. This process is characterized by either oscillations or stable induction of NF-kappaB nuclear binding. Changes in dynamics of binding result in the expression of distinct subsets of genes leading to different physiological outcomes. We examined NF-kappaB DNA binding activity in lipopolysaccharide (LPS)-stimulated IC-21 cells by electromobility shift assay and nonradioactive transcription factor assay and interpreted the results using a kinetic model of NF-kappaB activation. Both assays detected damped oscillatory behavior of NF-kappaB with differences in sensitivity and reproducibility. 3,4-Dichloropropionaniline (DCPA) was used to modulate the oscillatory behavior of NF-kappaB after LPS stimulation. DCPA is known to inhibit the production of two NF-kappaB-inducible cytokines, IL-6 and tumor necrosis factor alpha, by reducing but not completely abrogating NF-kappaB-induced transcription. DCPA treatment resulted in a potentiation of early LPS-induced NF-kappaB activation. The nonradioactive transcription factor assay, which has a higher signal/noise ratio than the electromobility shift assay, combined with in silico modeling, produced results that revealed changes in NF-kappaB dynamics which, to the best of our knowledge, have never been previously reported. These results highlight the importance of cell type and stimulus specificity in transcription factor activity assessment. In addition, assay selection has important implications for network inference and drug discovery.

  4. Quantitative Analysis of NF-κB Transactivation Specificity Using a Yeast-Based Functional Assay.

    Directory of Open Access Journals (Sweden)

    Vasundhara Sharma

    Full Text Available The NF-κB transcription factor family plays a central role in innate immunity and inflammation processes and is frequently dysregulated in cancer. We developed an NF-κB functional assay in yeast to investigate the following issues: transactivation specificity of NF-κB proteins acting as homodimers or heterodimers; correlation between transactivation capacity and in vitro DNA binding measurements; impact of co-expressed interacting proteins or of small molecule inhibitors on NF-κB-dependent transactivation. Full-length p65 and p50 cDNAs were cloned into centromeric expression vectors under inducible GAL1 promoter in order to vary their expression levels. Since p50 lacks a transactivation domain (TAD, a chimeric construct containing the TAD derived from p65 was also generated (p50TAD to address its binding and transactivation potential. The p50TAD and p65 had distinct transactivation specificities towards seventeen different κB response elements (κB-REs where single nucleotide changes could greatly impact transactivation. For four κB-REs, results in yeast were predictive of transactivation potential measured in the human MCF7 cell lines treated with the NF-κB activator TNFα. Transactivation results in yeast correlated only partially with in vitro measured DNA binding affinities, suggesting that features other than strength of interaction with naked DNA affect transactivation, although factors such as chromatin context are kept constant in our isogenic yeast assay. The small molecules BAY11-7082 and ethyl-pyruvate as well as expressed IkBα protein acted as NF-κB inhibitors in yeast, more strongly towards p65. Thus, the yeast-based system can recapitulate NF-κB features found in human cells, thereby providing opportunities to address various NF-κB functions, interactions and chemical modulators.

  5. Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization.

    Science.gov (United States)

    Cotelle, Sylvie; Dhyèvre, Adrien; Muller, Serge; Chenon, Pascale; Manier, Nicolas; Pandard, Pascal; Echairi, Abdelwahad; Silvestre, Jérôme; Guiresse, Maritxu; Pinelli, Eric; Giorgetti, Lucia; Barbafieri, Meri; Silva, Valéria C; Engel, Fernanda; Radetski, Claudemir M

    2015-01-01

    The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, "Assessment of genotoxic effects on higher plants-Vicia faba micronucleus test," for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials). The aim of this interlaboratory study on the Vicia micronucleus assay was to investigate the robustness of this in vivo assay in terms of its applicability in different countries where each participant were asked to use their own seeds and reference soil, in agreement with the ISO 29200 standard. The ISO 29200 standard protocol was adopted for this study, and seven laboratories from three countries (France, Italy, and Brazil) participated in the study. Negative and positive controls were correctly evaluated by 100 % of the participants. In the solid-phase test, the micronucleus frequency (number of micronuclei/1,000 cells) varied from 0.0 to 1.8 for the negative control (i.e., Hoagland's solution) and from 5.8 to 85.7 for the positive control (i.e., maleic hydrazide), while these values varied from 0.0 to 1.7 for the negative control and from 14.3 to 97.7 for the positive control in the case of liquid-phase test. The variability in the data obtained does not adversely affect the robustness of the protocol assessed, on the condition that the methodology described in the standard ISO 29200 is strictly respected. Thus, the Vicia micronucleus test (ISO 29200) is appropriate for complementing prokaryotic or in vitro tests cited in legislation related to risk assessment of genotoxicity potential.

  6. Validation of a single nucleotide polymorphism (SNP) typing assay with 49 SNPs for forensic genetic testing in a laboratory accredited according to the ISO 17025 standard

    DEFF Research Database (Denmark)

    Børsting, Claus; Rockenbauer, Eszter; Morling, Niels

    2009-01-01

    A multiplex assay with 49 autosomal single nucleotide polymorphisms (SNPs) developed for human identification was validated for forensic genetic casework and accredited according to the ISO 17025 standard. The multiplex assay was based on the SNPforID 52plex SNP assay [J.J. Sanchez, C. Phillips, C...

  7. 转录因子NF-κB 活性检测技术%Techniques for assaying the activity of transcription factor NF-κB

    Institute of Scientific and Technical Information of China (English)

    凌小倩; 王进科

    2013-01-01

    NF-κB activity have always been paid more attention and continuously developed. Especially in recent year, along with the development of each disciplines, various new techniques have been developed, including ELISA-like assays based on dsDNA-coupled plate, filter binding assays, FRET assays, fluorescence reporting and nucleic acids amplification assays based on exonuclease and endonuclease, MS and flow cytometry assays based on immunomicrobeads, and other biophysical and electrochemical assays. Some of these techniques have already played important roles in NF-κB studies. This paper reviewed new techniques developed in recent years by classification, in order to providean overview of NF-κB activity assays, which may be helpful for researchers to select appropriate techniques used in their studies. Moreover, the learning and understanding of these techniques may inspirere searchers to improve currently existing techniques and develop novel methods forthe studies of NF-κB.

  8. A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer.

    Science.gov (United States)

    Altevogt, Dominik; Hrenn, Andrea; Kern, Claudia; Clima, Lilia; Bannwarth, Willi; Merfort, Irmgard

    2009-10-07

    Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

  9. ISO 14001

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2004-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001, the international standard for environmental management systems (EMS). The standard is a process standard that leaves room for interpretation at company level as well as among lead auditors from certifying bodies....... The paper questions whether the interpretation of the standard is uniform both at national and international levels. The paper also suggests changes of ISO 14001 in order to formulate more clear demands of environmental improvements, to include demand for publication of an environmental report...... and to include product focus more clearly. Secondly the paper briefly presents a cases study of four companies in the automotive industry in South Africa, related to the discussions of ISO 14001 and compliance with environmental regulation....

  10. Learn about NF

    Science.gov (United States)

    ... Contact Donate Understanding NF Neurofibromatosis Intro to NF NF1 NF2 Schwannomatosis Genetics of NF Living with NF ... develop NF? Half of the people who develop NF1 or NF2 inherit it from a parent. The ...

  11. [Method validation according to ISO 15189 and SH GTA 04: application for the extraction of DNA and its quantitative evaluation by a spectrophotometric assay].

    Science.gov (United States)

    Harlé, Alexandre; Lion, Maëva; Husson, Marie; Dubois, Cindy; Merlin, Jean-Louis

    2013-01-01

    According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecular biology and specifically in somatic or constitutional genetic. The extracted DNA must meet a number of criteria such quality and also be in sufficient concentration to allow molecular biology assays such as the detection of somatic mutations. This paper describes the validation of the extraction and purification of DNA using chromatographic column extraction and quantitative determination by spectrophotometric assay, according to ISO 15189 and the accreditation technical guide in Human Health SH-GTA-04.

  12. ISO27001 / ISO27002 a pocket guide

    CERN Document Server

    Calder, Alan

    2013-01-01

    Information is one of your organisation's most important resources. Keeping it secure is therefore vital to your business. This handy pocket guide is an essential overview of two key information security standards that cover the formal requirements (ISO27001:2013) for creating an Information Security Management System (ISMS), and the best-practice recommendations (ISO27002:2013) for those responsible for initiating, implementing or maintaining it.

  13. NF EN ISO 11137-1, July 2006. Sterilization of health care products - Irradiation - Part 1: Requirements for development, validation and routine control of a sterilization process for medical devices; NF EN ISO 11137-1, Juillet 2006. Sterilisation des produits de sante - Irradiation - Partie 1: exigences relatives a la mise au point, a la validation et au controle de routine d'un procede de sterilisation pour les dispositifs medicaux

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This part of the ISO 11137 standard specifies requirements for the development, validation and routine control of a radiation sterilization process for medical devices. Although the scope of this part of the ISO 11137 standard is limited to medical devices, it specifies requirements and provides guidance that may be applicable to other products and equipment. This part of the international standard covers radiation processes employing irradiators using the radionuclide {sup 60}Co or {sup 137}Cs, a beam from an electron generator or a beam from an X-ray generator. This part of the international standard does not: - specify requirements for development, validation and routine control of a process for inactivating the causative agents of spongiform encephalopathies such as scrapie, bovine spongiform encephalopathy and Creutzfeldt-Jakob disease; - detail specified requirements for designating a medical device as sterile; - specify a quality management system for the control of all stages of production of medical devices; - specify requirements for occupational safety associated with the design and operation of irradiation facilities; - specify requirements for the sterilization of used or reprocessed devices.

  14. Analytical method for the identification and assay of 12 phthalates in cosmetic products: application of the ISO 12787 international standard "Cosmetics-Analytical methods-Validation criteria for analytical results using chromatographic techniques".

    Science.gov (United States)

    Gimeno, Pascal; Maggio, Annie-Françoise; Bousquet, Claudine; Quoirez, Audrey; Civade, Corinne; Bonnet, Pierre-Antoine

    2012-08-31

    Esters of phthalic acid, more commonly named phthalates, may be present in cosmetic products as ingredients or contaminants. Their presence as contaminant can be due to the manufacturing process, to raw materials used or to the migration of phthalates from packaging when plastic (polyvinyl chloride--PVC) is used. 8 phthalates (DBP, DEHP, BBP, DMEP, DnPP, DiPP, DPP, and DiBP), classified H360 or H361, are forbidden in cosmetics according to the European regulation on cosmetics 1223/2009. A GC/MS method was developed for the assay of 12 phthalates in cosmetics, including the 8 phthalates regulated. Analyses are carried out on a GC/MS system with electron impact ionization mode (EI). The separation of phthalates is obtained on a cross-linked 5%-phenyl/95%-dimethylpolysiloxane capillary column 30 m × 0.25 mm (i.d.) × 0.25 mm film thickness using a temperature gradient. Phthalate quantification is performed by external calibration using an internal standard. Validation elements obtained on standard solutions, highlight a satisfactory system conformity (resolution>1.5), a common quantification limit at 0.25 ng injected, an acceptable linearity between 0.5 μg mL⁻¹ and 5.0 μg mL⁻¹ as well as a precision and an accuracy in agreement with in-house specifications. Cosmetic samples ready for analytical injection are analyzed after a dilution in ethanol whereas more complex cosmetic matrices, like milks and creams, are assayed after a liquid/liquid extraction using ter-butyl methyl ether (TBME). Depending on the type of cosmetics analyzed, the common limits of quantification for the 12 phthalates were set at 0.5 or 2.5 μg g⁻¹. All samples were assayed using the analytical approach described in the ISO 12787 international standard "Cosmetics-Analytical methods-Validation criteria for analytical results using chromatographic techniques". This analytical protocol is particularly adapted when it is not possible to make reconstituted sample matrices.

  15. Identification and comparison of anti-inflammatory ingredients from different organs of Lotus nelumbo by UPLC/Q-TOF and PCA coupled with a NF-κB reporter gene assay.

    Directory of Open Access Journals (Sweden)

    Mengge Zhou

    Full Text Available Lotus nelumbo (LN (Nelumbo nucifera Gaertn. is an aquatic crop that is widely distributed throughout Asia and India, and various parts of this plant are edible and medicinal. It is noteworthy that different organs of this plant are used in traditional herbal medicine or folk recipes to cure different diseases and to relieve their corresponding symptoms. The compounds that are contained in each organ, which are named based on their chemical compositions, have led to their respective usages. In this work, a strategy was used to identify the difference ingredients and screen for Nuclear-factor-kappaB (NF-κB inhibitors with anti-inflammatory ability in LN. Seventeen main difference ingredients were compared and identified from 64 samples of 4 different organs by ultra-performance liquid chromatography that was coupled with quadrupole/time of flight mass spectrometry (UPLC/Q-TOF-MS with principal component analysis (PCA. A luciferase reporter assay system combined with the UPLC/Q-TOF-MS information was applied to screen biologically active substances. Ten NF-κB inhibitors from Lotus plumule (LP extracts, most of which were isoquinoline alkaloids or flavone C-glycosides, were screened. Heat map results showed that eight of these compounds were abundant in the LP. In conclusion, the LP extracts were considered to have the best anti-inflammatory ability of the four LN organs, and the chemical material basis (CMB of this biological activity was successfully validated by multivariate statistical analysis and biological research methods.

  16. Nieuwe ISO- normen melkinstallaties

    OpenAIRE

    Wemmenhove, H.; Huijsmans, P.J.M.

    1996-01-01

    Alle melkmachine-installaties die na 1 oktober 1996 worden aangelegd moeten voldoen aan nieuwe ISO-normen beschreven in TNA-'96. In tegenstelling tot de normen van 1984, zijn de nieuwe normen meer op het bedrijf toegeschreven.

  17. Early results from ISO

    Science.gov (United States)

    1996-01-01

    First findings by Europe's new space telescope ISO (Infrared Space Observatory) will be announced at a press conference to be held at ESA's satellite tracking station in Villafranca, Apartado 50727 - 28080-Madrid, on Wednesday 14 February 1996 Astronomers responsible for ISO's instruments will show results ranging from materials in the planet Saturn, through the birth and death of stars, to the behaviour of colliding galaxies. All instruments are working well and even their preliminary results confirm that ISO is a unique observatory making an unprecedented exploration of the universe by infrared rays. Parallel press conferences will be held at ESA Headquarters in Paris, ESTEC Noordwijk (the Netherlands) and ESOC Darmstadt (Germany) where a live television link will be established with Villafranca and from where the media can participate in the discussion.

  18. Impact of 50% Synthesized Iso-Paraffins (SIP) on Middle Distillate Fuel Filtration and Coalescence

    Science.gov (United States)

    2014-10-30

    Impact of 50% Synthesized Iso-Paraffins (SIP) on Middle Distillate Fuel Filtration and Coalescence NF&LCFT REPORT 441/15-003 30 October 2014...October 2014 Page 1 Impact of 50% Synthesized Iso-Paraffins (SIP) on Middle Distillate Fuel Filtration and Coalescence 1.0 BACKGROUND In...Paraffins (SIP) on Middle 5a. CONTRACT NUMBER N/A Distillate Fuel F-76 Filtration and Coalescence 5b. GRANT NUMBER N/A 5c. PROGRAM

  19. ISO CD 14492

    DEFF Research Database (Denmark)

    Martins, Bo; Kossentini, F.; Arps, R.

    1998-01-01

    This Recommendation | International Standard, informally called JBIG2, defines a coding method for bilevel images, that is, images consisting of a single rectangular bit plane, with each pixel taking on one of just two possible colors. Itis being drafted by the Joint Bi-level Image Experts Group ...... (JBIG), a "Collaborative team" that reports both to ISO/IEC JTC1/SC29/WG1 and to ITU-T/SG8....

  20. ISO 21500 AND PMBOK COMPARISON

    OpenAIRE

    Dotsenko A.V.

    2013-01-01

    On 31st December 2012, the Project Management Institute (PMI) published the 5th edition of the Project Management Body of Knowledge (PMBoK) Guide. A few months earlier, the International Organization for Standardization (ISO) published a new standard, ISO 21500:2012, Guidance on Project Management. The two releases of the PMBoK Guide and ISO 21500 standard are very close. Detailed review of the differences and similarities between them are compiled.

  1. ISO 9000 Quality Management System

    Science.gov (United States)

    Hadjicostas, Evsevios

    The ISO 9000 series describes a quality management system applicable to any organization. In this chapter we present the requirements of the standard in a way that is as close as possible to the needs of analytical laboratories. The sequence of the requirements follows that in the ISO 9001:2008 standard. In addition, the guidelines for performance improvement set out in the ISO 9004 are reviewed. Both standards should be used as a reference as well as the basis for further elaboration.

  2. ISO 50001 Energy Management Standard

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-08-12

    This powerful standard from the International Organization for Standardization (ISO) provides an internationally recognized framework for organizations to voluntarily implement an energy management system.

  3. ISO 19152: 2012, Land Administration Domain Model published by ISO

    NARCIS (Netherlands)

    Van Oosterom, P.; Lemmen, C.; Uitermark, H.

    2013-01-01

    This paper describes the last developments of the Land Administration Domain Model (LADM). The Final Draft International Standard, ISO FDIS 19152, unanimously passed on 1 November 2012 the final vote towards becoming an International Standard (IS). After technical editing by ISO secretariat in Genev

  4. ERP与ISO9000

    Institute of Scientific and Technical Information of China (English)

    李劫

    2003-01-01

    ERP作为企业管理信息化的重要手段,越来越受到企业管理者的关注;同时,以ISO9000为代表的现代质量管理方法也被越来越多的企业所采纳。在谈及企业管理行为时,无论采用何种管理方法,都应该是千条线进一根针,统一在企业管理的系统构架之下。企业管理好比一部运转着的机器,需要每一个环节,每一个部件的有效配合,

  5. ISO 9000: The Librarian's Role.

    Science.gov (United States)

    Dobson, Chris; Ernst, Carolyn

    1999-01-01

    Describes the special library's role in implementing ISO 9000 (i.e., a series of international quality-assurance standards developed by the International Organization of Standards). Topics discussed include document and data control, keeping the standards current, documentation of procedures, the ISO 9000 audit, and benefits for the library. (MES)

  6. ISO9000、BPR和ERP%ISO9000, BPR & ERP

    Institute of Scientific and Technical Information of China (English)

    施能自

    2002-01-01

    @@ 从目前情况来看,如何认识和处理ISO9000、BPR(业务流程重组)和ERP(企业资源计划系统)相互关系等问题困扰着不少中国企业的老总.如果企业已通过了ISO9000的认证,是否就意味着企业在管理方面已达到最高境界,可以一劳永逸,借助ISO9000的证书在竞争激烈的市场上通行无阻?在通过了ISO9000认证的前提下,如果再进行业务流程重组,是否会对ISO9000的复审带来中击?另外,由于ISO9000、BPR和ERP的实施工作都将产生操作手册、业务手册、程序文件等大量文档,是否会使得企业在大量文件中无所适从?

  7. ISO 9000与TQM

    Institute of Scientific and Technical Information of China (English)

    郑树海

    1998-01-01

    通过对ISO 9000与TQM的分析比较,阐明了两者在质量管理和质量保证的做法上的相同点和不同点,从而得出ISO 9000与TQM的侧重面不同,它们之间不能互相代替,只能起到互相补充的结论.

  8. ISO 9001 quality management systems

    CERN Document Server

    Natarajan, Dhanasekharan

    2017-01-01

    This book explains the requirements of ISO 9001 for establishing quality management system (QMS) for an organization. The requirements are illustrated with examples from industries for understanding the requirements and preparing the documents of QMS with high clarity. Methods of integrating ISO 9001 requirements with enterprise resource planning (ERP) software are presented. The software integrated approach enables process owners to focus on their core tasks of achieving the planned outputs of processes and the software generates quality records automatically.

  9. Moments of structure functions for $N_f=2$ near the physical point

    CERN Document Server

    Bali, G S; Gläßle, B; Göckeler, M; Najjar, J; Rödl, R; Schäfer, A; Schiel, R; Sternbeck, A; Söldner, W

    2013-01-01

    We report on our on-going study of the lower moments of iso-vector polarised and unpolarised structure functions, $g_A$ and $\\langle x\\rangle_{u-d}$, respectively, and the iso-vector scalar and tensor charge, for $N_f=2$ non-perturbatively improved clover fermions. With pion masses which go down to about 150 MeV, we investigate finite volume effects and excited state contributions.

  10. Nephrin Deficiency Activates NF-κB and Promotes Glomerular Injury

    Science.gov (United States)

    Hussain, Sagair; Romio, Leile; Saleem, Moin; Mathieson, Peter; Serrano, Manuel; Moscat, Jorge; Diaz-Meco, Maria; Scambler, Peter

    2009-01-01

    Increasing evidence implicates activation of NF-κB in a variety of glomerular diseases, but the mechanisms involved are unknown. Here, upregulation of NF-κB in the podocytes of transgenic mice resulted in glomerulosclerosis and proteinuria. Absence of the podocyte protein nephrin resulted in NF-κB activation, suggesting that nephrin negatively regulates the NF-κB pathway. Signal transduction assays supported a functional relationship between nephrin and NF-κB and suggested the involvement of atypical protein kinase C (aPKCζ/λ/ι) as an intermediary. We propose that disruption of the slit diaphragm leads to activation of NF-κB; subsequent upregulation of NF-κB-driven genes results in glomerular damage mediated by NF-κB-dependent pathways. In summary, nephrin may normally limit NF-κB activity in the podocyte, suggesting a mechanism by which it might discourage the evolution of glomerular disease. PMID:19497968

  11. Identification of a protein that interacts with the nuclear factor-1 (NF-1) binding site in cells that do not express NF-1: comparison to NF-1, cellular distribution, and effect on transcription.

    OpenAIRE

    McQuillan, J J; Rosen, G.D.; Birkenmeier, T M; Dean, D C

    1991-01-01

    We examined expression of nuclear factor-1 (NF-1) in different cell lines. Expression was low or undetectable in T and B lymphocyte cell lines, whereas fibroblasts and other adherent cell lines generally had a relatively high level of NF-1 mRNA. In cell lines that did not express NF-1, gel retardation assays, nevertheless, indicated complexes between a protein or proteins and the NF-1 site. These complexes were less abundant than those formed with NF-1, they migrated more slowly, and they app...

  12. Isoorientin attenuates lipopolysaccharide-induced pro-inflammatory responses through down-regulation of ROS-related MAPK/NF-κB signaling pathway in BV-2 microglia.

    Science.gov (United States)

    Yuan, Li; Wu, Yuchen; Ren, Xiaomeng; Liu, Qian; Wang, Jing; Liu, Xuebo

    2014-01-01

    Isoorientin (ISO) is a flavonoid compound in the human diet, and has been known to possess various bioactivities. However, the effects of ISO on microglia inflammation have not been investigated. The current study investigates the neuroprotective effect of ISO in LPS-activated mouse microglial (BV-2) cells. ISO significantly increased the BV-2 cells viability, blocked the protein expression of inducible nitric oxide synthase and cyclooxygenase-2, and decreased the production of nitric oxide, pro-inflammatory cytokines including tumor necrosis factor-α and interleukin-1β. The activation of mitogen-activated protein kinases (MAPKs) was blocked by ISO, and NF-κB nuclear translocation was decreased by ISO both alone and together with NF-κB inhibitor (PDTC) and MAPKs inhibitors (U0126, SP 600125, and SB 203580). Furthermore, ISO strongly quenched intracellular reactive oxygen species (ROS) generation. ROS inhibitor (N-acetyl cysteine, NAC) significantly inhibited pro-inflammatory cytokines release and NF-κB and MAPKs activation, indicating that ISO attenuated neuroinflammation by inhibiting the ROS-related MAPK/NF-κB signaling pathway.

  13. [ISO 15189 medical laboratory accreditation].

    Science.gov (United States)

    Aoyagi, Tsutomu

    2004-10-01

    This International Standard, based upon ISO/IEC 17025 and ISO 9001, provides requirements for competence and quality that are particular to medical laboratories. While this International Standard is intended for use throughout the currently recognized disciplines of medical laboratory services, those working in other services and disciplines will also find it useful and appropriate. In addition, bodies engaged in the recognition of the competence of medical laboratories will be able to use this International Standard as the basis for their activities. The Japan Accreditation Board for Conformity Assessment (AB) and the Japanese Committee for Clinical Laboratory Standards (CCLS) are jointly developing the program of accreditation of medical laboratories. ISO 15189 requirements consist of two parts, one is management requirements and the other is technical requirements. The former includes the requirements of all parts of ISO 9001, moreover it includes the requirement of conformity assessment body, for example, impartiality and independence from any other party. The latter includes the requirements of laboratory competence (e.g. personnel, facility, instrument, and examination methods), moreover it requires that laboratories shall participate proficiency testing(s) and laboratories' examination results shall have traceability of measurements and implement uncertainty of measurement. Implementation of ISO 15189 will result in a significant improvement in medical laboratories management system and their technical competence. The accreditation of medical laboratory will improve medical laboratory service and be useful for patients.

  14. α-Iso-cubebene exerts neuroprotective effects in amyloid beta stimulated microglia activation.

    Science.gov (United States)

    Park, Sun Young; Park, Se Jin; Park, Nan Jeong; Joo, Woo Hong; Lee, Sang-Joon; Choi, Young-Whan

    2013-10-25

    Schisandra chinensis is commonly used for food and as a traditional remedy for the treatment of neuronal disorders. However, it is unclear which component of S. chinensis is responsible for its neuropharmacological effects. To answer this question, we isolated α-iso-cubebene, a dibenzocyclooctadiene lignin, from S. chinensis and determined if it has any anti-neuroinflammatory and neuroprotective properties against amyloid β-induced neuroinflammation in microglia. Microglia that are stimulated by amyloid β increased their production of pro-inflammatory cytokines and chemokines, prostaglandin E2 (PGE2), nitric oxide (NO) and reactive oxygen species (ROS) and the enzymatic activity of matrix metalloproteinase 9 (MMP-9). We found this was all inhibited by α-iso-cubebene. Consistent with these results, α-iso-cubebene inhibited the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) and MMP-9 in amyloid β-stimulated microglia. Subsequent mechanistic studies revealed that α-iso-cubebene inhibited the phosphorylation and degradation of IκB-α, the phosphorylation and transactivity of NF-κB, and the phosphorylation of MAPK in amyloid β-stimulated microglia. These results suggest that α-iso-cubebene impairs the amyloid β-induced neuroinflammatory response of microglia by inhibiting the NF-κB and MAPK signaling pathways. Importantly, α-iso-cubebene can provide critical neuroprotection for primary cortical neurons against amyloid β-stimulated microglia-mediated neurotoxicity. To the best of our knowledge, this is the first report showing that α-iso-cubebene can provide neuroprotection against, and influence neuroinflammation triggered by, amyloid β activation of microglia.

  15. Environmental Management System ISO 14001

    CERN Document Server

    Haider, Syed

    2010-01-01

    This is a must-have tool for any company in the process of adopting and incorporating the ISO 14001:2004 requirements, this book and accompanying CD-ROM provides the latest updates and amendments and translates the ISO language into actionable strategy. Offering administrative solutions to managers of all sorts, it includes practical examples of policies with objectives, targets, and action plans applicable to any process related industry or an ordinary organization. With template formats and hands-on flow charts to describe step-by-step system development, documentation, and implementation ph

  16. ISO 14001: time for improvements?

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2007-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001:2004, the international standard for Environmental Management Systems (EMS) with the purpose of improving the next edition in order to recognise and reflect new recognitions in approaches to pollution prevention. A case...... study is presented and shows lack of life cycle thinking in product development. This paper suggests changes of ISO 14001:2004 in order to include a more product-oriented approach and a stronger focus on stakeholders. It also suggests to formulate clearer demands for targets and environmental...

  17. Information security risk management for ISO27001/ISO27002

    CERN Document Server

    Calder, A; Watkins, S

    2010-01-01

    Drawing on international best practice, including ISO/IEC 27005, NIST SP800-30 and BS7799-3, the book explains in practical detail how to carry out an information security risk assessment. It covers key topics, such as risk scales, threats and vulnerabilities, selection of controls, and roles and responsibilities, and includes advice on choosing risk assessment software.

  18. Our Application of ISO 9000.

    Science.gov (United States)

    Hammond, Jane

    2000-01-01

    Since a large, underfunded urban Colorado district initiated ISO 9000 reforms, administrators and staff have reviewed 14 central-office departments' processes to improve efficiency and enhance student outcomes. Jefferson County has saved $900,000 annually on purchasing processes, developed a quality curriculum-development process, and improved…

  19. ISO27001 in a Windows Environment

    CERN Document Server

    Honan, Brian

    2010-01-01

    Ideal for project managers, IT and security staff, this book plugs the gap in current guidance literature for ISO27001. ISO27001, the information security management standard (ISMS), is providing a significant challenge for many organisations. One of the key areas of confusion is the relationship between the ISO27001 ISMS project manager and those responsible for implementing the technical controls.

  20. The European Large Area ISO Survey

    DEFF Research Database (Denmark)

    Oliver, S.; Rowan-Robinson, M.; Alexander, D.M.

    2000-01-01

    We describe the European Large Area ISO Survey (ELAIS). ELAIS was the largest single Open Time project conducted by ISO, mapping an area of 12 deg(2) at 15 mu m with ISOCAM and at 90 mu m with ISOPHOT. Secondary surveys in other ISO bands were undertaken by the ELAIS team within the fields...

  1. DNA duplex stability of the thio-iso-guanine•methyl-iso-Cytosine base pair.

    Science.gov (United States)

    Lee, Dongkye; Switzer, Christopher

    2015-01-01

    We report the synthesis, incorporation into oligonucleotides, and base-pairing properties of the 2-thio-variant of iso-guanine. Iso-guanine is the purine component of a nonstandard base pair with 5-methyl-iso-cytosine. The 2-thio-iso-guanine • 5-methyl-iso-cytosine base pair is found to have similar stability to an adenine • thymine pair.

  2. Integrative Augmentation with the New ISO 10000 Standards1

    OpenAIRE

    Karapetrovic Stanislav

    2014-01-01

    Application and integration of the systems based on ISO 10001: 2007 and other new augmentative standards are discussed. Particular attention is paid to the quality management standards forming the front end of the ISO 10000 series, namely ISO 10001, ISO 10002 and ISO 10003, as well as ISO 19011, the standard which stems from the original augmentative ISO 10000 guideline, specifically ISO 10011 on quality auditing. The ability of these and other similar standards to augment the performance of ...

  3. ISO: highlights of recent results

    Science.gov (United States)

    Metcalfe, L.; Salama, A.

    ESA's Infrared Space Observatory (ISO) mission, operating in the wavelength range from 2.5 to 240 microns, made over 26000 scientific observations during its 2.5 year operational lifetime. ISO's results broke new ground on all scales. New asteroid counts and improved asteroid thermophysical models augmented important advances in Solar System chemistry to comprise a striking body of results addressing our planetary system. In turn, parallels between the chemical composition of Solar System dust and dust around other stars revealed by the comparison of stellar spectra with cometary spectra, together with results on the incidence and stability of stellar disks, recall the birth of our Solar System and point to fundamental similarities with other star systems. Numerous important facts concerning the chemistry of the ISM have unfolded, such as the ubiquity of water and of the probably-organic carriers of the Unidentified Infrared Bands (UIBs). The large systematic body of data on galactic stars has permitted fascinating advances in the characterisation of important aspects of stellar evolution. Investigations of nearby normal galaxies complement template specimens of interacting galaxies. These in turn exemplify galaxy evolutionary processes in the early Universe associated with a huge burst of dust-obscured star formation at redshifts of just below one. This global surge of star formation has vital implications for the interpretation and explanation of major components of the Extragalactic Background Light (EBL) and for charting the global history of star formation and the relative importance of sources which derive their energy from accretion processes. Representative examples of key aspects of ISO's recent scientific output will be presented, once again affirming ISO's place at the forefront of successful space-borne astronomy missions.

  4. ISO 17025简介

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    目前,大家对1SO9000、ISO14000、OHSAS18000等管理体系已不陌生.但对ISO17025可能就比较少接触。所谓ISO17025,就是由国际标准化组织/合格评定委员会(ISO/CASCO)制定的实验室管理标准,前身是ISO/IEC导则25:1990《校准和检测实验室能力的要求》。国际上对实验室认可进行管理的组织是“国际实验室认可合作组织(ILAC)”。由包括中国实验室国家认可委员会(CNACL)在内的44个实验室认可机构参加。ISO17025主要包括:定义、组织和管理、质量体系、审核和评审、人员、设施和环境、设备和标准物质、量值溯源和校准、校准和检测方法、样品管理、记录、证书和报告、校准或检测的分包、外部协助和供给、抱怨等内容。其核心内容为设备和标准物质、量值溯源和校准、校准和检测方法、样品管理。重点是评价实验室校准或检测能力是否达到预期的要求。

  5. 75 FR 7581 - RTO/ISO Performance Metrics; Notice Requesting Comments on RTO/ISO Performance Metrics

    Science.gov (United States)

    2010-02-22

    ... Energy Regulatory Commission RTO/ISO Performance Metrics; Notice Requesting Comments on RTO/ ISO..., work with regional transmission organizations (RTO), Independent System Operators (ISO), stakeholders and other experts to develop standardized measures that track the performance of RTO/ISO...

  6. Ny versjon av standarden ISO 15489

    Directory of Open Access Journals (Sweden)

    Herbjørn Andresen

    2016-09-01

    Full Text Available Standarden NS-ISO 15489-1:2016 er gjeldende norsk standard fra 1. juli 2016. Dette er andre versjon av ISO 15489, den første versjonen fra 2001 er ikke lenger gyldig. 2001-utgaven av ISO 15489 besto av to deler. Del 2, SN-ISO/TR 15489-2:2001 er trukket, slik at den nye ISO 15489 kun består av del 1. ISO 15489 var den første internasjonale Records Management-standarden, og har en funksjon som overordnet referansestandard for en voksende portefølje av senere standarder på tilgrensende områder. Denne artikkelen gjennomgår endringene i hovedtrekk, både i standarden ISO 15489 som sådan og i noen av de standardene som bygger videre på den.

  7. Cryogenic mechanism for ISO camera

    Science.gov (United States)

    Luciano, G.

    1987-12-01

    The Infrared Space Observatory (ISO) camera configuration, architecture, materials, tribology, motorization, and development status are outlined. The operating temperature is 2 to 3 K, at 2.5 to 18 microns. Selected material is a titanium alloy, with MoS2/TiC lubrication. A stepping motor drives the ball-bearing mounted wheels to which the optical elements are fixed. Model test results are satisfactory, and also confirm the validity of the test facilities, particularly for vibration tests at 4K.

  8. 什么是ISO 9000?

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

      ISO 9000是国际标准化组织(英文简称ISO)于1987年3月订立的一种产品质量保证制度国际标准,它又被称为ISO 9000系列质量管理及质量保证标准指导纲要.……

  9. ISO/IEC 27001与ISO/IEC 27002标准的演变%Introduction of ISO/IEC 27001 and ISO/IEC 27002

    Institute of Scientific and Technical Information of China (English)

    谢宗晓; 王静漪

    2015-01-01

    本文按照时间顺序梳理了ISO/IEC 27001和ISO/IEC 27002的发展过程,其中包括这两个标准成为国际标准的开发过程以及被等同采用为我国国家标准的过程。%We reorganized history of ISO/IEC 27001 and ISO/IEC 27002 in chronological order, includingdevelopment process before becoming international standard and the process of adoption as Chinese standards.

  10. VizieR Online Data Catalog: ISO Observation Log (ISO Team, 2001)

    Science.gov (United States)

    Iso Data, Centre

    2001-04-01

    The Infrared Space Observatory (ISO) is an ESA (European Space Agency) project with instruments funded by ESA member states (especially the PI countries France, Germany, The Netherlands and the United Kingdom) and with participation of ISAS (Japan) and NASA (USA). It was operated from November 1995 till May 1998, almost a year longer than expected. As an unprecedented observatory for infrared astronomy, able to examine cool and hidden places in the Universe, ISO successfully made nearly 30,000 scientific observations. The ISO data can be retrieved from the ISO Data Archive, available at the ISO Data Centre at http://www.iso.vilspa.esa.es, comprising about 140,000 observations, including parallel mode and serendipity observations. ISO helpdesk: helpdesk@iso.vilspa.esa.es (1 data file).

  11. Implementation and Impact of ISO 9001

    NARCIS (Netherlands)

    B. Manders (Basak)

    2015-01-01

    markdownabstract__Abstract __ The ISO 9001 quality management standard has been implemented by more than one million organizations in 187 countries since its introduction in 1987. A newer version of ISO 9001 is planned to be introduced in 2015. Even though it is widely used by organizations to gai

  12. Affinity-matured recombinant immunotoxin targeting gangliosides 3'-isoLM1 and 3',6'-isoLD1 on malignant gliomas.

    Science.gov (United States)

    Piao, Hailan; Kuan, Chien-Tsun; Chandramohan, Vidya; Keir, Stephen T; Pegram, Charles N; Bao, Xuhui; Månsson, Jan-Eric; Pastan, Ira H; Bigner, Darell D

    2013-01-01

    About 60 percent of glioblastomas highly express the gangliosides 3'-isoLM1 and 3',6'-isoLD1 on the cell surface, providing ideal targets for brain tumor immunotherapy. A novel recombinant immunotoxin, DmAb14m-(scFv)-PE38KDEL (DmAb14m-IT), specific for the gangliosides 3'-isoLM1 and 3',6'-isoLD1, was constructed with improved affinity and increased cytotoxicity for immunotherapeutic targeting of glioblastoma. We isolated an scFv parental clone from a previously established murine hybridoma, DmAb14, that is specific to both 3'-isoLM1 and 3',6'-isoLD1. We then performed in vitro affinity maturation by CDR hotspot random mutagenesis. The binding affinity and specificity of affinity-matured DmAb14m-IT were measured by surface-plasmon resonance, flow cytometry, and immunohistochemical analysis. In vitro cytotoxicity of DmAb14m-IT was measured by protein synthesis inhibition and cell death assays in human cell lines expressing gangliosides 3'-isoLM1 and 3',6'-isoLD1 (D54MG and D336MG) and xenograft-derived cells (D2224MG). As a result, the KD of DmAb14m-IT for gangliosides 3'-isoLM1 and 3',6'-isoLD1 was 2.6 × 10(-9)M. Also, DmAb14m-IT showed a significantly higher internalization rate in cells expressing 3'-isoLM1 and 3',6'-isoLD1. The DmAb14m-IT IC 50 was 80 ng/mL (1194 pM) on the D54MG cell line, 5 ng/ml (75 pM) on the D336MG cell line, and 0.5 ng/ml (7.5 pM) on the D2224MG xenograft-derived cells. There was no cytotoxicity on ganglioside-negative HEK293 cells. Immunohistochemical analysis confirmed the specific apparent affinity of DmAb14m-IT with 3'-isoLM1 and 3',6'-isoLD1. In conclusion, DmAb14m-IT showed specific binding affinity, a significantly high internalization rate, and selective cytotoxicity on glioma cell lines and xenograft-derived cells expressing 3'-isoLM1 and 3',6'-isoLD1, thereby displaying robust therapeutic potential for testing the antitumor efficacy of DmAb14m-IT at the preclinical level and eventually in the clinical setting.

  13. VizieR Online Data Catalog: ISO Observation Log (ISO Data Centre, 2004)

    Science.gov (United States)

    ISO Data Centre, 2004

    2004-04-01

    The Infrared Space Observatory (ISO) is an ESA (European Space Agency) project with instruments funded by ESA member states (especially the PI countries France, Germany, The Netherlands and the United Kingdom) and with participation of ISAS (Japan) and NASA (USA). It was operated from November 1995 till May 1998, almost a year longer than expected. As an unprecedented observatory for infrared astronomy, able to examine cool and hidden places in the Universe, ISO successfully made nearly 30,000 scientific observations. The ISO Data Archive is available at the ISO Data Centre at http://www.iso.vilspa.esa.es and it includes about 150,000 observations, if account is taken of calibration and parallel or serendipity mode observations. The catalogue contains all observations performed in standard observing modes exempt from technical problems; special flags indicate calibration observations. ISO helpdesk: helpdesk@iso.vilspa.esa.es (1 data file).

  14. VizieR Online Data Catalog: ISO Observation Log of validated data (ISO Data Centre, 2001)

    Science.gov (United States)

    ISO Data Centre

    2001-06-01

    The Infrared Space Observatory (ISO) is an ESA (European Space Agency) project with instruments funded by ESA member states (especially the PI countries France, Germany, The Netherlands and the United Kingdom) and with participation of ISAS (Japan) and NASA (USA). It was operated from November 1995 till May 1998, almost a year longer than expected. As an unprecedented observatory for infrared astronomy, able to examine cool and hidden places in the Universe, ISO successfully made nearly 30,000 scientific observations. The ISO data can be retrieved from the ISO Data Archive, available at the ISO Data Centre at http://www.iso.vilspa.esa.es, comprising about 150,000 observations, including parallel and serendipity mode observations. This catalogue contains a subset of the science observations, exempt from technical problems and performed in standard observing modes, for which the automated pipeline processing products have been declared as scientifically validated by the instrument teams. The catalogue gives observation details and provides links to quick-look images depicting the data and to observation specific documentation. The final version of the ISO automated Offline Processing Software and associated Calibration files (OLP 10.0 and CALG 7.0) is available through the on-the-fly-reprocessing facility of the ISO Data Archive since June 8, 2001. By the end of 2001, all ISO telemetry will have been reprocessed with this version of the pipeline software, and the ISO Data Archive will have been repopulated with the latest version of the products. During ISO's Active Archive Phase, ending in 2006, effort will be invested to augment the archive content for many observations by ingestion of products which become available through interactive or specialised processing of subsets of the ISO data. So the archive will continue to be improved in terms of both its user friendliness, and the immediacy with which products can be taken and used. ISO helpdesk: helpdesk@iso

  15. ISO Science Legacy A Compact Review of ISO Major Achievements

    CERN Document Server

    Cesarsky, Catherine J

    2006-01-01

    Stars are born and die in clouds of gas and dust, opaque to most types of radiation, but transparent in the infrared. Requiring complex detectors, space missions and cooled telescopes, infrared astronomy is the last branch of this discipline to come of age. After a very successful sky survey performed in the eighties by the IRAS satellite, the Infrared Space Observatory, in the nineties, brought spectacular advances in the understanding of the processes giving rise to powerful infrared emission by a great variety of celestial sources. Outstanding results have been obtained on the bright comet Hale-Bopp, and in particular of its water spectrum, as well as on the formation, chemistry and dynamics of planetary objects in the solar system. Ideas on the early stages of stellar formation and on the stellar initial mass function have been clarified. ISO is the first facility in space able to provide a systematic diagnosis of the physical phenomena and the chemistry in the close environment of pre-main sequence stars...

  16. Effectiveness of ISO 9001 System Implementation in Japanese Companies

    OpenAIRE

    Shimada,Tomoaki; Okamoto, Nobuki

    2009-01-01

    ISO (International Organization for Standardization) management systems are represented by ISO 9001:2000. Since the establishment of the ISO 9000 series in 1984, many manufacturers in Japan that perform international transactions have adopted the ISO management systems, and now, companies in various industries, including those in the service sector, have implemented ISO management systems. The ISO 9001 system specifies operational procedures that reflect business policy, organizational charac...

  17. Phenotypic characterization of transgenic mice harboring Nf1+/- or Nf1-/- osteoclasts in otherwise Nf1+/+ background.

    Science.gov (United States)

    Alanne, Maria H; Siljamäki, Elina; Peltonen, Sirkku; Väänänen, Kalervo; Windle, Jolene J; Parada, Luis F; Määttä, Jorma A; Peltonen, Juha

    2012-06-01

    Skeletal abnormalities in neurofibromatosis type 1 syndrome (NF1) are observed in ∼50% of patients. Here, we describe the phenotype of Nf1(Ocl) mouse model with Nf1-deficient osteoclasts. Nf1Ocl mice with Nf1+/- or Nf1-/- osteoclasts in otherwise Nf1+/+ background were successfully generated by mating parental Nf1flox/flox and TRAP-Cre mice. Contrary to our original hypothesis, osteoporotic or fragile bone phenotype was not observed. The µCT analysis revealed that tibial bone marrow cavity, trabecular tissue volume, and the perimeter of cortical bone were smaller in Nf1 Ocl-/- mice compared to Nf1 Ocl+/+ control mice. Nf1 Ocl-/- mice also a displayed narrowed growth plate in the proximal tibia. In vitro analysis showed increased bone resorption capacity and cytoskeletal changes including irregular cell shape and abnormal actin ring formation in Nf1-/- osteoclasts. Surprisingly, the size of spleen in Nf1 Ocl-/- mice was two times larger than in controls and histomorphometric analysis showed splenic megakaryocytosis. In summary, Nf1Ocl mouse model presented with a mild but specific bone phenotype. This study shows that NF1-deficiency in osteoclasts may have a role in the development of NF1-related skeletal abnormalities, but Nf1-deficiency in osteoclasts in Nf1+/+ background is not sufficient to induce skeletal abnormalities analogous to those observed in patients with NF1.

  18. ISO 9001 2000 : the quality management process

    CERN Document Server

    Tricker, Ray

    2006-01-01

    With the publication of ISO 9001:2000, there is now a single quality management ?requirements? standard that is applicable to all organisations, products and services. ISO 9001:2000 is the only standard that can be used for the certification of a QMS and its generic requirements can be used by any organisation.ISO 9001:2000 applies to all types or organisations. It is the quality standard which specifies the requirements of quality management systems for use where organisations need to demonstrate their capability to provide products and services which meet both customer needs and relevant reg

  19. Involvement of the NF-κB signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model.

    Science.gov (United States)

    Qiu, Shujuan; Sun, Guiling; Zhang, Yunxia; Li, Xiangling; Wang, Rong

    2016-05-01

    The aim of the present study was to investigate the renoprotective effects of isorhamnetin (ISO) in type 2 diabetic rats and its effects on the nuclear factor-κB (NF-κB) signaling pathway, which is associated with diabetic nephropathy. The type 2 diabetic rat model was established by a high-fat diet plus streptozocin injection and the rats were subsequently treated with two dosages of ISO, respectively. The levels of blood glucose were determined. Urinary osteopontin, kidney injury molecule-1 (KIM-1) and albumin were measured to evaluate the renal function of the rats. Renal NF-κB signaling activity was assessed by measuring the levels of NF-κB p65, phospho-NF-κB p65, inhibitor of NF-κB (IκBα) and phospho-IκBα, and the NF-κB p65 DNA-binding activity. Downstream inflammatory mediators [tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, intercellular adhesion molecule-1 (ICAM-1) and transforming growth factor-β1 (TGF-β1)] of the NF-κB signaling pathway were investigated to evaluate the renal inflammatory response. Renal levels of malondialdehyde and total superoxide dismutase were detected to access the oxidative stress. Furthermore, glomerular mesangial cells (GMCs) were treated with lipopolysaccharide and ISO. In the cellular experiment, the NF-κB signaling activity, levels of TNF-α, IL-1β, IL-6, ICAM-1 and TGF-β1, and oxidative stress were also investigated. The results showed that ISO decreased the levels of urinary osteopontin, KIM-1 and albumin. ISO also inhibited the NF-κB signaling activity, decreased the production of inflammatory mediators and attenuated oxidative stress in diabetic rats and GMCs. The present investigations revealed that ISO had ameliorative effects on diabetes-induced renal damage and the activity may be associated with the negative regulation of NF-κB signaling pathway.

  20. Relationship between the Expression of NF-kappa B and Apoptosis in Non-Small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    Jianqun Ma; Zhenfa Zhang; Shidong Xu

    2008-01-01

    OBJECTIVE To study the expression of the nuclear factor kappa B (NF-kappa B)in non-small cell lung cancer(NSCLC),to explore the apoptotic ratio in NSCLC related to different NF-kappa Bs,and to understand the clinical significance of NF-kappa B in NSCLC apoptosis.METHODS NF-kappa B expression in 45 new samples of NSCLC,collected during a period from October to December,2005,was assayed using Western blots,and the apoptotic ratio of NSCLC was determined by the Tunel method.RESULTS Of the 45 patients,the average relative expressxon of NF-kappa B was 0.6047±0.3572.The expression of NF-kappa B was higher in the poorly differentiated lung cancer cells than in the well-differentiated tumors (P<0.05).The apoptotic ratio was 56.4%in the lung cancer cells with higher NF-kappa B expression,and was 76.7%in those with lower NF-kappa B expression(P<0.05).CONCLUSION The expression of NF-kappa B is correlated with the differentiation of NSCLC.NF-kappa B inhibits apoptosis in NSCLC.AS a transcription factor,NF-kappa B plays a very important role both in formation and in development of NSCLC.NF-kappa B might serve as a target for NSCLC gene therapy.

  1. ISO 14001 ENVIRONMENTAL MANAGEMENT SYSTEMS FOR GOVERNMENT

    Science.gov (United States)

    This presentation provides an overview of the document, ISO 14001 - A Tool for Supporting Government Environmental Programs and Policies, recently published by USEPA/s Office of Research and Development. It is an informational document, explaining the backjground and development ...

  2. Implement an ISO 14001 environmental management system

    Energy Technology Data Exchange (ETDEWEB)

    Schiffman, R.I.; Delaney, B.T.; Fleming, S.; Hamilton, E.

    1997-11-01

    The ISO 14001 Environmental Management System (EMS) standard brings environmental concerns into the mainstream of business operations by providing a framework for balancing and integrating environmental and economic interests. ISO 14001 requires that an organization develop an environmental management program that describes all of its environmental objectives and targets and how each will be achieved. The program must include a specific plan that details the actions required to meet each objective and target, the person(s) responsible for meeting each objective, and a time scale for when each target will be attained. Objectives and targets can be prioritized within the program, but all objectives must be included. This article outlines the benefits of developing an EMS, explains the ISO 14001 EMS standard, and provides detailed step-by-step guidance on what to do to become ISO 14001 certified.

  3. "ISO/IEC 17025"与"ISO 9000"的区别与联系

    Institute of Scientific and Technical Information of China (English)

    汤洁

    2002-01-01

    本文归纳了ISO/IEC 17025和ISO 9000系列标准的基本含义和技术要求,进一步阐明了它们之间的区别和联系,并对我国已获得"17025"和"ISO 9000"证书的部分检验实验室和生产企业的基本状况进行了分析.

  4. P systems generating iso-plcture languages

    Institute of Scientific and Technical Information of China (English)

    Subbaiah Annadurai; Thiyagarajan Kalyani; Vincent Rajkumar Dare; Durairaj Gnanaraj Thomas

    2008-01-01

    P systems generating rectangular arrays and hexagonal arrays have been studied in the literature,bringing together the two areas of theoretical computer science,namely membrane computing and picture languages.Recently,a new class of picture languages called the class of iso-picture languages generating interesting picture languages has been introduced.In this paper,we develop a class of tissue-like P systems with active membranes as a generative device for iso-picture languages.

  5. ISO9001和ISO/TS16949之联系和差异%The Relationship and Differences between ISO 9001 and ISO/TS 16949

    Institute of Scientific and Technical Information of China (English)

    马学辉

    2010-01-01

    文章主要介绍了ISO9001和ISO/TS16949质量管理体系要求之间的关系和差异以及它们的核心要求和适用性.本文同时介绍了对于它们的认证机构在管理上的差异.

  6. 建立ISO 14001 / ISO 9001一体化管理体系

    Institute of Scientific and Technical Information of China (English)

    付海湘

    2001-01-01

    建立ISO 14001 / ISO 9001环境/质量一体化管理体系是企业管理体系的发展方向。文章从建立ISO 14001 / ISO 9001环境/质量一体化管理体系的优势、途径、文件结构内容、建议的环境/质量手册目次以及必要的程序文件清单等方面进行了总结、介绍。

  7. Benefits of the ISO 9001 and ISO 14001 standards: A literature review

    Directory of Open Access Journals (Sweden)

    Juan José Tarí

    2012-12-01

    Full Text Available Purpose: The purpose of this paper is to determine the similarities and differences between the benefits derived from implementing the ISO 9001 and the ISO 14001 standards.Methodology/Approach: The paper reviews the literature using an electronic search in the ScienceDirect, ABI/Inform, Emerald databases to identify papers focusing on the adoption of the ISO 9001 and 14001 standards and the benefits derived from implementing them.Findings: The paper identifies 82 articles about ISO 9001 and 29 about ISO 14001. Although some differences can be observed between the benefits considered by ISO 9001 and 14001, there is a great degree of coincidence in the benefits studied. The review suggests 13 benefits as the most usually analyzed (including environmental performance for the case of the ISO 14001 standard by scholars. It is suggested that both standards have clear benefits on operational, people and customer results and that the effects on financial performance are inconclusive.Limitations/implications: One limitation of this paper is that the works identified are conditioned by the search strategy used. In addition, other key words could be included in future studies such as operational, market, quality, financial performance, and customer satisfaction in order to expand this search.Originality/Value: The main contribution is that the paper identifies the literature gap and future research proposals with regard to the benefits of the ISO 9001 and ISO 14001 standards.

  8. Activation of NF-κB and apoptosis of intestinal epithelial cells induced by hydrogen peroxide

    Institute of Scientific and Technical Information of China (English)

    李建明; 周红; 蔡黔; 肖光夏

    2002-01-01

    In vitro model of hydrogen peroxide induced apoptosis of SW-480 cells was used to investigate the role of NF-κB in the pathogenesis of reactive oxygen species induced apoptosis of intestinal epithelial cells. Methods: Ultra-structural changes were observed.Apoptosis of SW-480 cell line was determined by Annexin-V and PI double-stained flow cytometry. Nuclear translocation of NF-κB was determined by anti-NF-κB polyclonal antibody and EB double-staining. NF-κB activity was studied by electrophoretic mobility shift assays. RTPCR was performed to study expression of NF-κB mRNA. Results: Hydrogen peroxide led to apoptosis of SW-480 cells, condensed or semilunar chromatin even apoptotic bodies could be observed. Nuclear translocation of NF-κB,increase of NF-κB activity and expression of NF-κB mRNA were found simultaneously. Conclusions: Early activation of NF-κ B may be one of the mechanisms of apoptosis in intestinal epithelial cells by reactive oxygen species.

  9. Aplikace ISO norem v oblasti vývoje softwaru

    OpenAIRE

    Ullrich, Jan

    2007-01-01

    Thesis evaluates ISO standars used for software developement and their relationship to methodologies. It also describes processes according to international standard ISO/IEC 12207 and quality management systems according to ISO 9000. It evaluates also mothodlogy RUP and agile methodollogies. The goal of the thesis to tailor processes related to ISO 9001 to be compliant to the ISO/IEC 12207 in a small company.

  10. ISO 31000的整合实施%An Integrated Implementation of ISO 31000

    Institute of Scientific and Technical Information of China (English)

    吕多加; 高晓红

    2013-01-01

    The article describes a generic implementation process for risk management that integrates the risk management framework and process described in ISO 31000. According to the process the organizations are able to effectively implement risk management ISO 31000, adapted to their unique circumstances.

  11. ISO 9000 and Creativity: Potential Advantages of Implementing ISO in Community Colleges

    Science.gov (United States)

    Alkeaid, Adel

    2007-01-01

    The purpose of this paper is to examine the relationship between the ISO 9000 standard and creativity. In this paper, the researcher tries to answer the following questions: Which learning theories best foster creative thought? Is creativity more behaviorist or constructivist? Where does ISO 9000 fit in the continuum from behaviorism to…

  12. Transforming the Land Administration Domain Model (LADM) into an ISO Standard (ISO19152)

    NARCIS (Netherlands)

    Lemmen, C.H.J.; Van Oosterom, P.J.M.; Uitermark, H.T.; Thompson, R.J.; Hespanha, J.P.

    2009-01-01

    In February 2008, FIG submitted the Land Administration Domain Model (LADM) to the International Organization for Standardization (ISO). After three meetings with the LADM/ISO19152 Project Team (in Copenhagen, Denmark, May 2008; in Delft, The Netherlands, September 2008; and in Tsukuba, Japan, Decem

  13. Expression of NF-κB and osteopontin of synovial fluid of patientswith knee osteoarthritis

    Institute of Scientific and Technical Information of China (English)

    Li-Feng Qin; Dan Peng; Wan-Chun Wang; Han Fang; Xin-Zhan Mao; Guo-Liang Huang; You Chen; Hou-De Zhou; Yi Shen; Li-Hua Qin

    2013-01-01

    Objective: To explore the significance of osteopontin and nuclear factorκB (NF-κB) expression in patients with knee osteoarthritis. Methods: RT-PCR and enzyme-linked immunosorbent assay were used to measure the Osteopontin (OPN) and NF-κB concentration of knee joint synovial fluid of patients with knee osteoarthritis and trauma fractures, and analyze the relationship between the expressiones of them. Results: OPN and NF-κB expression at the mRNA and protein levels of patients with knee osteoarthritis were significantly higher than the control group. the result showed statistical significance (P<0.05). There was a positive correlation between the OPN levels in synovial fluid of patients with knee osteoarthritis and NF-κB expression levels (P<0.05). Conclusions: The high expression of OPN and NF-κB are closely related to occurrence and development of knee osteoarthritis.

  14. Involvement of activation of the Nrf2/ARE pathway in protection against 6-OHDA-induced SH-SY5Y cell death by α-iso-cubebenol.

    Science.gov (United States)

    Park, Sun Young; Kim, Do Yeon; Kang, Jong-Koo; Park, Geuntae; Choi, Young-Whan

    2014-09-01

    Free radical-mediated neurodegeneration is one of the many causes of Parkinson's disease (PD). As part of our ongoing studies on the identification of biologically active Schisandra chinensis components, we have isolated and structurally elucidated α-iso-cubebenol. This study was carried out in an attempt to clarify the neuroprotective effect of α-iso-cubebenol on toxin-insulted dopaminergic neuronal death using 6-hydroxy-dopamine (6-OHDA)-induced dopaminergic SH-SY5Y cells. α-iso-cubebenol significantly attenuated the loss of mitochondrial function (MTT assay) and membrane integrity (lactate dehydrogenase assay) associated with 6-OHDA-induced neurotoxicity. Pretreatment of the cells with α-iso-cubebenol diminished the intracellular accumulation of reactive oxygen species (ROS) and calcium in response to 6-OHDA. Moreover, α-iso-cubebenol protected against 6-OHDA-induced neurotoxicity through inhibition of SH-SY5Y cell apoptosis. In addition, JC-1 staining, which is a well-established measure of mitochondrial damage, was decreased after treatment with α-iso-cubebenol. Notably, α-iso-cubebenol inhibited the release of mitochondrial flavoprotein apoptosis inducing factor (AIF) from the mitochondria to the cytosol and nucleus following 6-OHDA treatment. In addition, α-iso-cubebenol reduced the 6-OHDA-induced phosphorylation of ERK and induced the phosphorylation of PKA, PKB, and CREB in a dose-dependent manner. Moreover, α-iso-cubebenol stimulated the activation of Nrf2, a downstream target of CREB. Furthermore, α-iso-cubebenol stimulated the expression of multiple antioxidant response genes (NQO-1 and HO-1). Finally, CREB and Nrf2 siRNA transfection diminished α-iso-cubebenol-mediated neuroprotection.

  15. ISO 9001:能否更好

    Institute of Scientific and Technical Information of China (English)

    杰克·戴林; 王金德

    2007-01-01

    作为质量行业的专业人员,我们能给自己、公司、顾客和我们的团队提出比ISO 9001标准更高的质量要求。许多才华横溢、工作努力、目标明确的人员,通过ISO 9001标准来控制和改进质量。但是如果抛弃ISO 9001,特别是第三方审核的一些特征,我们可能会做得更好。

  16. IS ISO 9000 CERTIFICATION LOSING ITS CREDIBILITY?

    Directory of Open Access Journals (Sweden)

    P.R. Ramesh

    2013-03-01

    Full Text Available ISO certification was a great hope for many stakeholders in India for making the country strong in terms of quality practices. It was a blessing in disguise when in early nineties; buyers were imposing conditions that you should be ISO compliant to do business with them. ISO was a great system to bring in improvements in the way an organization functions. But unfortunately some have adopted a short cut approach in getting the certification done. It is only just before the audit team comes for re-inspection that firms start removing dust from the quality manual files so that they can pretend that that they follow those systems. But a real commitment to adhering to these systems is missing. The paper describes such scenario existing in some quarters in India and warns against such practices for the overall good of the system.

  17. Isorhapontigenin (ISO) inhibited cell transformation by inducing G0/G1 phase arrest via increasing MKP-1 mRNA Stability.

    Science.gov (United States)

    Gao, Guangxun; Chen, Liang; Li, Jingxia; Zhang, Dongyun; Fang, Yong; Huang, Haishan; Chen, Xiequn; Huang, Chuanshu

    2014-05-15

    The cancer chemopreventive property of Chinese herb new isolate isorhapontigenin (ISO) and mechanisms underlying its activity have never been explored. Here we demonstrated that ISO treatment with various concentrations for 3 weeks could dramatically inhibit TPA/EGF-induced cell transformation of Cl41 cells in Soft Agar assay, whereas co-incubation of cells with ISO at the same concentrations could elicit G0/G1 cell-cycle arrest without redundant cytotoxic effects on non-transformed cells. Further studies showed that ISO treatment resulted in cyclin D1 downregulation in dose- and time-dependent manner. Our results indicated that ISO regulated cyclin D1 at transcription level via targeting JNK/C-Jun/AP-1 activation. Moreover, we found that ISO-inhibited JNK/C-Jun/AP-1 activation was mediated by both upregulation of MKP-1 expression through increasing its mRNA stability and deactivating MKK7. Most importantly, MKP-1 knockdown could attenuate ISO-mediated suppression of JNK/C-Jun activation and cyclin D1 expression, as well as G0/G1 cell cycle arrest and cell transformation inhibition, while ectopic expression of FLAG-cyclin D1 T286A mutant also reversed ISO-induced G0/G1 cell-cycle arrest and inhibition of cell transformation. Our results demonstrated that ISO is a promising chemopreventive agent via upregulating mkp-1 mRNA stability, which is distinct from its cancer therapeutic effect with downregulation of XIAP and cyclin D1 expression.

  18. Mechanisms of antiprostate cancer by gum mastic: NF-κB signal as target

    Institute of Scientific and Technical Information of China (English)

    Mei-lan HE; Ang LI; Chun-su XU; Shun-li WANG; Meng-jie ZHANG; Hua GU; Yao-qin YANG; Hui-hong TAO

    2007-01-01

    Aim: To study the effect of gum mastic, a natural resin, on the proliferation of androgen-independent prostate cancer PC-3 cells, and further investigate the mechanisms involved in this regulatory system, taking nuclear factor kB (NF-kB) signal as the target. Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and a flow cytometer were used to detect the effect of gum mastic on the proliferation of PC-3 cells. Then, reporter gene assay, RT-PCR, and Western blotting were carried out to study the effects of gum mastic on the NF-κB protein level and the NF-kB signal pathway. The expression of genes involved in the NF-kB signal pathway, including cyclin D1, inhibitors of kBs (IkBα), and phosphorylated Akt (p-AKT), were measured. In addition, transient transfection assays with the 5×NF-κB consensus sequence promoter was also used to test the effects of gum mastic. Results: Gum mastic inhibited PC-3 cell growth and blocked the PC-3 cell cycle in the G1 phase. Gum mastic also suppressed NF-κB activity in the PC-3 cells. The expression of cyclin D1, a crucial cell cycle regulator and an NF-kB downstream target gene, was reduced as well. Moreover, gum mastic decreased the p-AKT protein level and increased the IkBα protein level.Conclusion: Gum mastic inhibited the proliferation and blocked the cell cycle progression in PC-3 cells by suppressing NF-κB activity and the NF-κB signal pathway.

  19. miR-107 regulates tumor progression by targeting NF1 in gastric cancer.

    Science.gov (United States)

    Wang, Shizhi; Ma, Gaoxiang; Zhu, Haixia; Lv, Chunye; Chu, Haiyan; Tong, Na; Wu, Dongmei; Qiang, Fulin; Gong, Weida; Zhao, Qinghong; Tao, Guoquan; Zhou, Jianwei; Zhang, Zhengdong; Wang, Meilin

    2016-11-09

    Our previous genome-wide miRNA microarray study revealed that miR-107 was upregulated in gastric cancer (GC). In this study we aimed to explore its biological role in the pathogenesis of GC. Integrating in silico prediction algorithms with western blotting assays revealed that miR-107 inhibition enhanced NF1 (neurofibromin 1) mRNA and protein levels, suggesting that NF1 is one of miR-107 targets in GC. Luciferase reporter assay revealed that miR-107 suppressed NF1 expression by binding to the first potential binding site within the 3'-UTR of NF1 mRNA. mRNA stable assay indicated this binding could result in NF1 mRNA instability, which might contribute to its abnormal protein expression. Functional analyses such as cell growth, transwell migration and invasion assays were used to investigate the role of interaction between miR-107 and its target on GC development and progression. Moreover, We investigated the association between the clinical phenotype and the status of miR-107 expression in 55 GC tissues, and found the high expression contributed to the tumor size and depth of invasion. The results exhibited that down regulation of miR-107 opposed cell growth, migration, and invasion, whereas NF1 repression promoted these phenotypes. Our findings provide a mechanism by which miR-107 regulates NF1 in GC, as well as highlight the importance of interaction between miR-107 and NF1 in GC development and progression.

  20. Natural chalcones as dual inhibitors of HDACs and NF-κB

    OpenAIRE

    2012-01-01

    Histone deacetylase enzymes (HDACs) are emerging as a promising biological target for cancer and inflammation. Using a fluorescence assay, we tested the in vitro HDAC inhibitory activity of twenty-one natural chalcones, a widespread group of natural products with well-known anti-inflammatory and antitumor effects. Since HDACs regulate the expression of the transcription factor NF-κB, we also evaluated the inhibitory potential of the compounds on NF-κB activation. Only four chalcones, isoliqui...

  1. ISO 9001:Gurantee for exportion%ISO 9001:为输出提供保障

    Institute of Scientific and Technical Information of China (English)

    Jack West; 赵敏

    2009-01-01

    @@ ISO 9001标准要求产品设计应得到确认以确保其能够满足既定用途的要求,识别并满足顾客要求是ISO 9001贯穿始终的主题.例如,管理评审的一个预期输出是对不满足顾客要求的产品所做出的相关决定.附表是ISO 9001中的条款,这些都证实了输出的重要性.

  2. Baicalein inhibits TNF-α-induced NF-κB activation and expression of NF-κB-regulated target gene products.

    Science.gov (United States)

    Li, Junbo; Ma, Juan; Wang, Ke Si; Mi, Chunliu; Wang, Zhe; Piao, Lian Xun; Xu, Guang Hua; Li, Xuezheng; Lee, Jung Joon; Jin, Xuejun

    2016-11-01

    The nuclear factor-κB (NF-κB) transcription factors control many physiological processes including inflammation, immunity, apoptosis and angiogenesis. In our search for NF-κB inhibitors from natural resources, we identified baicalein from Scutellaria baicalensis as an inhibitor of NF-κB activation. As examined by the NF-κB luciferase reporter assay, we found that baicalein suppressed TNF-α-induced NF-κB activation in a dose-dependent manner. It also inhibited TNF-α-induced nuclear translocation of p65 through inhibition of phosphorylation and degradation of IκBα. Furthermore, baicalein blocked the TNF-α-induced expression of NF-κB target genes involved in anti-apoptosis (cIAP-1, cIAP-2, FLIP and BCL-2), proliferation (COX-2, cyclin D1 and c-Myc), invasion (MMP‑9), angiogenesis (VEGF) and major inflammatory cytokines (IL-8 and MCP1). The flow cytometric analysis indicated that baicalein potentiated TNF-α-induced apoptosis and induced G1 phase arrest in HeLa cells. Moreover, baicalein significantly blocked activation of p38, extracellular signal-regulated kinase 1/2 (ERK1/2). Our results imply that baicalein could be a lead compound for the modulation of inflammatory diseases as well as certain cancers in which inhibition of NF-κB activity may be desirable.

  3. ISO/IEC 17025与ISO/IEC导则25的比照分析%Contrast and Analysis of ISO/IEC 17025 with ISO/IEC Guide 25

    Institute of Scientific and Technical Information of China (English)

    吴听; 齐建华

    2001-01-01

    本文主要介绍ISO/IEC 17025:1999的由来,及其与ISO 9001、ISO 9002的关系;ISO/IEC 17025的组成和构架以及与ISO/IEC导则25的对照、比较和分析。以帮助读者了解ISO/IEC 17025:1999与导则25的异同,从而有助于推进ISO/IEC 17025的实施。%In order to help the readers with understanding the similarities and differences between ISO/IEC 17025:1999 and Guide 25 and thereby carry forward the implement of ISO/IEC 17025,the oringin of ISO/IEC 17025,its relationship with ISO 9001 and ISO 9002,the composition,frame and analysis of ISO/IEC 17025 and its contrast and comparision with Guide 25 are mainly described in this paper.

  4. ISO 19512: The land administration domain model

    NARCIS (Netherlands)

    Lemmen, C.H.J.; Van Oosterom, P.J.M.

    2011-01-01

    Focus of this paper is on the Land Administration Domain Model which is under development as an International Standard at ISO. This development is an initiative of the International Federation of Surveyors – FIG. The International Standard is expected to be published in 2012. Why is this development

  5. ISO sertifikaat aitab otsustada / Toomas Udu

    Index Scriptorium Estoniae

    Udu, Toomas

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti : Transport i logistika 25. mai lk. 8. Teenuse kvaliteedi näitajad logistikafirmas ISO standardi alusel. Vt. samas: Eestis kasutatakse kolme kvaliteedistandardit; Kuidas mõjutada kvaliteeti läbi juhtimissüsteemi

  6. New ISO standards for hearing protectors (A)

    DEFF Research Database (Denmark)

    Poulsen, Torben

    2000-01-01

    Working Group 17 under ISO/TC43/SC1 deals with measurement of hearing protector attenuation. WG17 is presently involved in revision of the present 4869-1 standard (especially sound field requirements and reproducibility estimations) and formulation of a new standard where inexperienced hearing...

  7. Enthusiasm for Europe's space telescope ISO

    Science.gov (United States)

    1996-11-01

    "Are the most luminous galaxies powered by extreme rates of star formation, or do they harbour black-hole-powered active nuclei as well?" Scientists lead by Reinhard Genzel at Max Planck Institut fur extra terrestrische Physik in Garching, Germany, have used ISO's Short Wavelength Spectrometer to give an answer. Writing in the London journal Nature, an independent commentator, Gerry Gilmore of the Institute of Astronomy in Cambridge, considers that their papers in Astronomy and Astrophysics give a clear answer to this question. The "remarkable result" according to Gilmore is that none of three ultra-luminous infrared galaxies studied by ISO requires an active nucleus to account for the emissions, which arise from rapid star formation. About another ISO target Gilmore writes: "In one lovely example, of two intersecting disk galaxies known as the Antennae, it is even possible to resolve the spot where the two disks currently cross, and to see the progression of star formation across the disk as the two galaxies orbit through each other." (Nature, 21 November 1996, p. 211) One year after its launch, ISO is exceptionally popular among astronomers, not only in Europe but worldwide. A panel of American astronomers, reporting to NASA on the scientific merits of eight astrophysical space missions, gives ISO the highest ranking and calls it "the major infrared mission of the decade". At a time when NASA is reducing its budgets for some astrophysical missions, its funding of ISO-related research is increasing. The demand for a share in ISO's unique view of the infrared universe is insatiable. ESA has been overwhelmed by new proposals for observations, coming from 511 groups of astronomers in ESA's own Member States and in the USA and Japan. Although ISO is extremely efficient, performing an average of 45 observations a day, it could not cope with 16,000 observations requested as additions to ISO's already busy programme for 1997. The time allocation committee has had to turn

  8. Clinical significance of the detection of 8-isoPG in exhaled breath condensate of different lung diseases%EBC中8-isoPG测定在不同肺部疾病中的临床意义

    Institute of Scientific and Technical Information of China (English)

    杨猛; 陶一江; 陈建荣; 朱杰; 陈金亮; 姚苏梅

    2013-01-01

    目的 研究ARDS、OSAHS和AECOPD患者的EBC中8-isoPG浓度变化及临床意义.对象和方法选择江苏省南通大学第二附属医院的ARDS、OSAHS和AECOPD患者,收集ARDS患者入院第一天、OSAHS患者晨起、AECOPD患者以及健康对照组的EBC;采用ELISA法检测EBC中的8-isoPG浓度.结果 1.ARDS组、OSAHS组、AECOPD组和健康对照组EBC中 8-isoPG浓度为[(52.19±11.51 ng/L)、(14.93±1.39 ng/L)、(6.74±3.38 ng/L)、(3.21±0.97 ng/L)];2.ARDS、OSAHS、AECOPD患者EBC中8-isoPG均高于健康对照组,三者EBC中8-isoPG浓度顺序排列为:ARDS组>OSAHS组>AECOPD组(P<0.01).结论 EBC中8-isoPG可以反应多种肺部疾病的炎症及氧化应激程度.%Objective To investigate the rliniral significance of the detection of 8-iso-prostaglandin ( 8-isoPG ) in exhaled breath condensate of patients with anute respiratory distress syndrome ( ARDS ), obstructive sleep apnea/hypopnea syndrome ( OSAHS ) and a-cute exacerbation of chronic obstructive pulmonary disease ( AECOPD ). Methods The EBC was collected from the patients with AR DS, OSAHS, AECOPD and healthy controlis, and the level of 8-isoPG was measured by enzyme linked immunosorbent assay. Results The concentrations of 8-isoPG in EBC of all patients and the healthy controls were 52. 19 ± 1 1. 51 ng/L( ARDS ), 14. 93 ± 1. 39 ng/L ( OSAHS), 6.74 ±3.38 ng/L( AECOPD), and 3. 21 ±0.97 ng/L( healthy controls). The levels of 8-isoPC in all patients were higher than that in the healthy controls. Conclusion The level of 8-isoPG in EBC can reflect the inflammation of different lung diseases and the degree of oxidative stress.

  9. COMPARISON METHODOLOGIES FOR CALIBRATION OF Hp(10) PERSONAL DOSEMETERS USING ISO 4037 AND ISO 29661 STANDARDS.

    Science.gov (United States)

    Cardoso, J; Santos, L; Carvalhal, G; Oliveira, C

    2016-09-01

    The calibration of electronic personal dosemeters at the Portuguese ionizing radiation metrology laboratory uses the standard IEC 61526 for calibration methodology. This standard describes the irradiation geometry for testing and indicates that the standard ISO 4037-1, 2, 3 and 4 should be used. The ISO 4037 establishes that the reference point of test is a point in the radiation monitor, known or established, and the calibration phantom should be placed on its back in order to simulate the trunk body. Recently, ISO published another standard, the ISO 29661, that changes the reference point from the radiation monitor to the front face of the calibration phantom. The aim of this work is to present the result of the comparison of these two methodologies on personal dosemeters from five different manufacturers. The work shows differences in the Hp(10) response up to 4% resulting from the two different reference point concepts.

  10. STUDI TEORITIS ISO 9000 DAN TQM: SUBSTITUSI ATAU KOMPLEMETER?

    Directory of Open Access Journals (Sweden)

    Harianto Respati

    2007-09-01

    Full Text Available Banyak pertentangan pendapat tentang dampak ISO 9000 dan TQM dalam kaitannya dengan tujuannya yakni kepuasan pelanggan. Selain itu peran ISO 9000 dan TQM dipandang masih membingungkan. Penjelasan pada jurnal ini, diuraikan secara konseptual baik dari pernyataan para pakar maupun hasil-hasil penelitian terdahulu tentang peran ISO 9000 dan TQM. Hasil menujukkan bahwa secara umum ISO 9000 dan TQM mempunyai tujuan yang sama yakni kepuasan pelanggan. Peran implementasi sertifikasi ISO 9000 dan praktek TQM adalah komplementer artinya ISO 9000 dan TQM merupakan sistem yang terpadu serta bekerja sama untuk mencapai sasaran yakni kepuasan pelanggan. Kajian teoritis tentang peran ISO 9000 dan TQM bertujuan untuk mempertegas keberadaan ISO 9000 dan TQM untuk kepentingan para peneliti, akademik maupun para praktisi

  11. Integrative Augmentation with the New ISO 10000 Standards1

    Directory of Open Access Journals (Sweden)

    Karapetrovic Stanislav

    2014-11-01

    Full Text Available Application and integration of the systems based on ISO 10001: 2007 and other new augmentative standards are discussed. Particular attention is paid to the quality management standards forming the front end of the ISO 10000 series, namely ISO 10001, ISO 10002 and ISO 10003, as well as ISO 19011, the standard which stems from the original augmentative ISO 10000 guideline, specifically ISO 10011 on quality auditing. The ability of these and other similar standards to augment the performance of quality management systems in organizations and to foster integration of the respective augmentative systems themselves or within the overarching management systems is addressed and further illustrated through an example of their use in engineering education.

  12. From ISO 14001 to EMAS%从ISO 14001到EMAS

    Institute of Scientific and Technical Information of China (English)

    徐新宇; 王文

    2014-01-01

    ISO14001 is currently the most widely used environmental management system standard, while EMAS is a voluntary environmental management tool released by EU legislation. Centering on the aspects of environmental performance, environmental assessment, environmental factors, information disclosure, compliance, employee involvement, etc., the paper analyzes the correlation of the two major differences between ISO14001 and EMAS environmental management tools, providing reference for organizations to upgrade from ISO14001 to EMAS.%ISO 14001是目前应用最为广泛的环境管理体系标准,而EMAS是欧盟法令发布的自愿性环境管理工具。本文围绕环境绩效、环境评审、环境因素、信息公开、全面合规性、员工参与等方面,分析ISO 14001和EMAS两种环境管理工具的相关性和主要差异,为组织从ISO 14001升级至EMAS提供参考。

  13. Suppressing progress of pancreatitis through selective inhibition of NF-κB activation by using NAC

    Institute of Scientific and Technical Information of China (English)

    赵志成; 郑树森; 陈文亮; 王选; 齐莹

    2004-01-01

    Objective: To explore the characteristics of NF-κB activation in the progress of pancreatitis, the relationship with expression of TNF-α in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC. Method: Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A). NAC served as the inhibitor of NF-κB activation. In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-κB activation was detected with flow cytometry (FCM) and the expression of TNF-α mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. Result: NF-κB activation in the pancreatitis group was higher than that in the control group (P<0.01), peaked at 3 hours, and was depressed by the inhibitor of NF-κB, NAC. The expression of TNF-α as well as the level of lipase and amylase in the serum also rose synchronously with activation of NF-κB. In contrast to group A, it was significantly different (P<0.01) in group B. After using NAC in group C, all of these values were decreased and the inflammatory reaction in the pancreas abated evidently. The pathology changes of the pancreas were shown to be alleviated in group C. Conclusion: First, NF-κB activity is intensively initiated in the course of pancreatitis and shown to have closely relationship with the release of cytokines. Second, use of NAC markedly depressed NF-κB activation. TNF-α expression is down regulated by cytokines. It is suggested that NAC probably acts as a useful agent for treatment of pancreatitis by indirectly inhibiting activation of NF-κB.

  14. Proposal for revision of ISO 4377: Flat-V weirs

    NARCIS (Netherlands)

    Kalkwijk, J.P.T.; Lianxiang, W.

    1983-01-01

    In ISO/TC 113/SC 2/308 it was suggested that ISO 4377 could be significantly simplified as far as the calculation of discharge was involved. The proposal for revision is based upon this suggestion. The revision is fully based upon the material given in ISO 4377. In that respect no changes have been

  15. The Relationship between the Training Function and ISO-9000 Registration.

    Science.gov (United States)

    Maxwell, Randy C.; Jost, Karen L.

    ISO 9000 is one of a series of international standards providing guidelines and governing quality of products and services. The ISO 9000 certification demonstrates the capability of a supplier to control the processes that determine the acceptability of the product or service being supplied. This paper focuses on the training aspects of ISO 9000…

  16. 什么是ISO 9000族标准?

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    "ISO 9000族"是国际标准化组织(International Organization for Stan-dardization,简称 ISO)在1994年提出的概念,是指"由国际标准化组织质量管理和质量保证技术委员会(ISO/TC

  17. An introduction to information security and ISO27001:2013

    CERN Document Server

    Watkins, Steve

    2013-01-01

    Up to date with the latest version of the Standard (ISO27001:2013), An Introduction to information security and ISO27001:2013 is the perfect solution for anyone wanting an accurate, fast, easy-to-read primer on information security from an acknowledged expert on ISO27001.

  18. Signal Transducer and Activator of Transcription (STAT)-3 Activates Nuclear Factor (NF)-κB in Chronic Lymphocytic Leukemia Cells

    Science.gov (United States)

    Liu, Zhiming; Hazan-Halevy, Inbal; Harris, David M.; Li, Ping; Ferrajoli, Alessandra; Faderl, Stefan; Keating, Michael J.; Estrov, Zeev

    2014-01-01

    Nuclear factor (NF)-κB plays a major role in the pathogenesis of B-cell neoplasms. A broad array of mostly extracellular stimuli has been reported to activate NF-κB, to various degrees, in chronic lymphocytic leukemia (CLL) cells. Because CLL cells harbor high levels of unphosphorylated (U) signal transducer and activator of transcription (STAT)-3 protein and U-STAT3 was reported to activate NF-κB, we sought to determine whether U-STAT3 activates NF-κB in CLL. Using the electrophoretic mobility shift assay (EMSA) we studied peripheral blood low-density cells from 15 patients with CLL and found that CLL cell nuclear extracts from all the samples bound to an NF-κB DNA probe, suggesting that NF-κB is constitutively activated in CLL. Immunoprecipitation studies showed that STAT3 bound NF-κB p65, and confocal microscopy studies detected U-STAT3/NF-κB complexes in the nuclei of CLL cells, thereby confirming these findings. Furthermore, infection of CLL cells with retroviral STAT3-shRNA attenuated the binding of NF-κB to DNA, as assessed by EMSA, and downregulated mRNA levels of NF-κB-regulated genes, as assessed by quantitative polymerase chain reaction. Taken together, our data suggest that U-STAT3 binds to the NF-κB p50/p65 dimers and that the U-STAT3/NF-κB complexes bind to DNA and activate NF-κB-regulated genes in CLL cells. PMID:21364020

  19. ISO's analysis of Comet Hale-Bopp

    Science.gov (United States)

    1997-03-01

    The European Space Agency's Infrared Space Observatory ISO inspected Comet Hall-Bopp during the spring and autumn of 1996. The need to keep ISO's telescope extremely cold restricts the spacecraft's pointing in relation to the Sun and the Earth and it ruled out observations at other times. The analyses of the 1996 observations are not yet complete, but already they give new insight into the nature of comets. Comet Hale-Bopp is believed to be a large comet with a nucleus up to 40 kilometres wide. It was discovered in July 1995 by two American astronomers working independently, Alan Hale and Thomas Bopp. At that time, the comet was a billion kilometres away from the Sun, but 200 times brighter than Halley's Comet was, when at a comparable distance. Comet Hale-Bopp will make its closest approach to the Earth on 22 March, and its closest approach to the Sun (perihelion) on 1 April 1997. Some scientific results from ISO The discovery of Comet Hale-Bopp occurred before ISO's launch in November 1995. When first observed by ISO in March and April 1996, the comet was still 700 million kilometres from the Sun, and almost as far from the Earth and ISO. With its privileged view of infrared wavebands inaccessible from the Earth's surface, ISO's photometer ISOPHOT discovered that carbon dioxide was an important constituent of the comet's emissions of vapour.ISOPHOT measured the temperature of the dust cloud around Comet Hale-Bopp. In March 1996, when the comet was still more than 700 million kilometres from the Sun, the dust cloud was at minus 120 degrees C. When ISOPHOT made similar observations in October 1996, the comet was 420 million kilometres from the Sun, and the dust cloud had warmed to about minus 50 degrees C. Intensive observations of Comet Hale-Bopp were also made by ISO's Short-Wave Spectrometer SWS, the Long-Wave Spectrometer LWS, and the ISOPHOT spectrometer PHOT-S. Results are due for publication at the end of March. They will give details about the composition

  20. ISO Results Presented at International Astronomical Union

    Science.gov (United States)

    1997-08-01

    Some of the work being presented is collected in the attached ESA Information Note N 25-97, ISO illuminates our cosmic ancestry. A set of six colour images illustrating various aspects have also been released and are available at http://www.estec.esa.nl/spdwww/iso1808.htm or in hard copy from ESA Public Relations Paris (fax:+33.1.5369.7690). These pictures cover: 1. Distant but powerful infrared galaxies 2. A scan across the milky way 3. Helix nebula: the shroud of a dead star 4. Supernova remnant Cassiopeia A 5. Trifid nebula: a dusty birthplace of stars 6. Precursors of stars and planets The International Astronomical Union provides a forum where astronomers from all over the world can develop astronomy in all its aspects through international co-operation. General Assemblies are held every three years. It is expected that over 1600 astronomers will attend this year's meeting, which is being held in Kyoto, Japan from 18-30 August. Further information on the meeting can be found at: www.tenmon.or.jp/iau97/ . ISO illuminates our cosmic ancestry The European Space Agency's Infrared Space Observatory, ISO, is unmatched in its ability to explore and analyse many of the universal processes that made our existence possible. We are children of the stars. Every atom in our bodies was created in cosmic space and delivered to the Sun's vicinity in time for the Earth's formation, during a ceaseless cycle of birth, death and rebirth among the stars. The most creative places in the sky are cool and dusty, and opaque even to the Hubble Space Telescope. Infrared rays penetrating the dust reveal to ISO hidden objects, and the atoms and molecules of cosmic chemistry. "ISO is reading Nature's recipe book," says Roger Bonnet, ESA's director of science. "As the world's only telescope capable of observing the Universe over a wide range of infrared wavelengths, ISO plays an indispensable part in astronomical discoveries that help to explain how we came to exist." This Information Note

  1. A Realization of Hom-Lie Algebras by Iso-Deformed Commutator Bracket

    OpenAIRE

    Xiuxian Li

    2013-01-01

    We construct classical Iso-Lie and Iso-Hom-Lie algebras in $gl(V)$ by twisted commutator bracket through Iso-deformation. We prove that they are simple. Their Iso-automorphisms and isotopies are also presented.

  2. Ras-Mek-Erk signaling regulates Nf1 heterozygous neointima formation.

    Science.gov (United States)

    Stansfield, Brian K; Bessler, Waylan K; Mali, Raghuveer; Mund, Julie A; Downing, Brandon D; Kapur, Reuben; Ingram, David A

    2014-01-01

    Neurofibromatosis type 1 (NF1) results from mutations in the NF1 tumor-suppressor gene, which encodes neurofibromin, a negative regulator of diverse Ras signaling cascades. Arterial stenosis is a nonneoplastic manifestation of NF1 that predisposes some patients to debilitating morbidity and sudden death. Recent murine studies demonstrate that Nf1 heterozygosity (Nf1(+/-)) in monocytes/macrophages significantly enhances intimal proliferation after arterial injury. However, the downstream Ras effector pathway responsible for this phenotype is unknown. Based on in vitro assays demonstrating enhanced extracellular signal-related kinase (Erk) signaling in Nf1(+/-) macrophages and vascular smooth muscle cells and in vivo evidence of Erk amplification without alteration of phosphatidylinositol 3-kinase signaling in Nf1(+/-) neointimas, we tested the hypothesis that Ras-Erk signaling regulates intimal proliferation in a murine model of NF1 arterial stenosis. By using a well-established in vivo model of inflammatory cell migration and standard cell culture, neurofibromin-deficient macrophages demonstrate enhanced sensitivity to growth factor stimulation in vivo and in vitro, which is significantly diminished in the presence of PD0325901, a specific inhibitor of Ras-Erk signaling in phase 2 clinical trials for cancer. After carotid artery injury, Nf1(+/-) mice demonstrated increased intimal proliferation compared with wild-type mice. Daily administration of PD0325901 significantly reduced Nf1(+/-) neointima formation to levels of wild-type mice. These studies identify the Ras-Erk pathway in neurofibromin-deficient macrophages as the aberrant pathway responsible for enhanced neointima formation.

  3. Identification of a NF-kB site in the negative regulatory element (εNRAII) of human ε-globin gene and its binding protein NF-κB p50 in the nuclei of K562 cells

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The developmental control of the human ε-globin gene expression is mediated by transcription regulatory elements in the 5' flanking DNA of this gene. Sequence analysis has revealed a DNA motif (GGGGAATTTGCT) similar to NF-κB consensus sequence resides in the negative regulatory element (-3028bp ~ -2902bp, termed ε-NRAII) 5' to the cap site of this gene. NRF DNA fragment (-3010bp ~-2986bp) containing the NF-κB motif similar sequence was synthesized and used in electrophoresis mobility shift assay (EMSA) and competitive analysis. Data showed that a protein factor from nuclear extracts of K562 cells specifically interacted with NRF DNA fragment. The synthetic NF DNA fragment (containing NF-κB consensus sequence) could competed for the protein binding, but MNF DNA fragment (mutated NF-κB motif) could not, suggesting that the binding protein is a member of NF-κB/Rel family. Western blot assay demonstrated that the molecular weight of NF-κB protein in the nuclei of K562 cells is 50ku. We suggested that NF-κB p50 may play an important role in the regulation of human ε-globin gene expression.

  4. Promoting Strong ISO 50001 Outcomes with Supportive National Infrastructure

    Energy Technology Data Exchange (ETDEWEB)

    McKane, Aimee, T.; Siciliano, Graziella; de los Reyes, Pamela

    2015-08-04

    The ISO 50001 standard is a key mechanism for reducing greenhouse gas emissions and improving energy efficiency globally. An increasing number of companies are seeking certification, creating the need for personnel that are competent to conduct ISO 50001 certification audits. The growth of ISO 50001 is expected to accelerate as more companies integrate ISO 50001 into their corporate sustainability strategies and supplier requirements. Robust implementation of ISO 50001 represents an important tool for countries with climate change mitigation goals. Because of its dual focus on continual improvement of an organization’s energy management system (EnMS) and its energy performance improvement, ISO 50001 requires skills of both implementers and certification auditors that are not well-supported by current credentials and training. This paper describes an effort to address skill gaps of certification auditors, a critical factor to ensure that ISO 50001 implementations are robust and result in continued energy performance improvement. A collaboration of governments through the Energy Management Working Group (EMWG), formerly under Global Superior Energy Performance (GSEP), has formed to build workforce capacity for ISO 50001 certification audits. The EMWG is leading the development of an internationally-relevant certification scheme for ISO 50001 Lead Auditor that meets requirements for ISO/IEC 17024 accreditation and ISO 50003 for defining ISO 50001 Lead Auditor competency. Wider availability of competent ISO 50001 Lead Auditors will ultimately increase the impact and market value of ISO 50001 certification and improve consistency of ISO 50001 certification outcomes by establishing a standardized and high level of knowledge and skills globally.

  5. Incorporating ISO Metadata Using HDF Product Designer

    Science.gov (United States)

    Jelenak, Aleksandar; Kozimor, John; Habermann, Ted

    2016-01-01

    The need to store in HDF5 files increasing amounts of metadata of various complexity is greatly overcoming the capabilities of the Earth science metadata conventions currently in use. Data producers until now did not have much choice but to come up with ad hoc solutions to this challenge. Such solutions, in turn, pose a wide range of issues for data managers, distributors, and, ultimately, data users. The HDF Group is experimenting on a novel approach of using ISO 19115 metadata objects as a catch-all container for all the metadata that cannot be fitted into the current Earth science data conventions. This presentation will showcase how the HDF Product Designer software can be utilized to help data producers include various ISO metadata objects in their products.

  6. Catalogue of ISO LWS observations of asteroids

    CERN Document Server

    Hormuth, Felix

    2009-01-01

    (Abridged) The Long Wavelength Spectrometer (LWS) onboard the Infrared Space Observatory (ISO) observed the four large main-belt asteroids (1) Ceres, (2) Pallas, (4) Vesta, and (10) Hygiea multiple times. The photometric and spectroscopic data cover the wavelength range between 43 and 197 um, and are a unique dataset for future investigations and detailed characterisations of these bodies. The standard ISO archive products, produced through the last post-mission LWS pipeline, were still affected by instrument artefacts. Our goal was to provide the best possible data products to exploit the full scientific potential of these observations. We performed a refined reduction of all measurements, corrected for various instrumental effects, and re-calibrated the data. We outline the data reduction process and give an overview of the available data and the quality of the observations. We apply a thermophysical model to the flux measurements to derive far-IR based diameter and albedo values of the asteroids. The measu...

  7. Serie de Normas NTP ISO 9000:2001

    OpenAIRE

    García P, Manuel; Quispe A, Carlos; FII-UNMSM; Ráez Guevara, Luis; FII-UNMSM

    2014-01-01

    La diferencia entre la serie de Normas NTP ISO 9000:1994 y la NTP ISO 9000:2001 es que la primera prioriza el aseguramiento, y la segunda el mejoramiento. en el presente artículo se pretende ilustrar los principales cambios y beneficios que trae consigo la nueva versión de la Serie de Normas NTP ISO 9000:2001 The difference between the number of ISO 9000:1994 Standards NTP and NTP ISO 9000:2001 is the first priority assurance, and the second improvement. in this article is intended to ill...

  8. ISO 9000: 2000: towards a learning organization ?

    Directory of Open Access Journals (Sweden)

    Michel DOMENC

    2009-01-01

    Full Text Available The 2000 version of ISO 9000 contains major changes compared to the 1994 version. We hypothesize that the changes it induces are in line of research applying the emergence of a new "model" organization. From a field survey among actors involved in implementing the new standard, the research presented examines changes recorded in terms of spaces of communication and cooperation, competence, shared responsibilities. These changes involved the development of a learning organization.

  9. Revision of ISO 15859 Aerospace Fluid Standards

    Science.gov (United States)

    Greene, Benjamin; McClure, Mark B.

    2012-01-01

    A detailed review of ISO 15859 "Space Systems - Fluid Characteristics, Sampling and Test Methods" was performed An approach to revising Parts 1-9 and 11-13 was developed and concurred by the NASA Technical Standards Program Office. The approach was to align them with the highest level source documents, and not to program-specific requirements. The updated documents were prepared and presented.

  10. "ENVIRONMENTAL AUDITING STANDARDS ISO14010, 14011, 14012 "

    OpenAIRE

    1998-01-01

    In order to standardize sustainable status of environmental management in productive and industrial activities and commercial and economical relationship, the ISO 14000 standards series guarantee the minimization of the costs, growth accoptancy of society and trade and environmental quality improvement and natural resources. In fact, these standards will help any organization to improve and solve these standards and help to improve and solve their environmental remarks of executive activities...

  11. 2000版ISO 9000修订概要

    Institute of Scientific and Technical Information of China (English)

    于立梅

    2000-01-01

    ISO 9000族的四个核心标准的修订目前进展得非常顺利。ISO 9000、ISO 9001及ISO 9004作为国际标准草案(DIS)已于1999年11月25日公布,期望在2000年第四季度以成熟的国际标准的形式公布于众。 ISO 19011(即质量与环境管理体系审核标准)已形成委员会草案,将取代目前ISO 9000和ISO 14000族的“单一范围”的审核标准,期望在2001年第四季度以国际标准的形式公布于众。

  12. Regulation of MSK1-Mediated NF-κB Activation Upon UVB Irradiation.

    Science.gov (United States)

    Carpenter, Oliver L; Wu, Shiyong

    2014-01-01

    Nuclear Factor Kappa-B (NF-κB) is a transcription factor that controls expression of genes involved in the immune and inflammatory responses as well as being a key component in the onset of cancers. In this study, we provided evidence that mitogen- and stress-activated protein kinase (MSK1) is responsible for a noncanonical late-phase activation of NF-κB upon UVB irradiation. Our data demonstrated that following UVB irradiation, MSK1 is activated via phosphorylation at the 24 h time point coinciding with translocation of NF-κB into the nucleus. Investigations into the signaling pathways upstream of MSK1 through the use of specific inhibitors for mitogen-activated protein kinase and p38 revealed that both kinases are required for full phosphorylation during the late phase (24 h), while p38 is paramount for phosphorylation during the early phase (6 h). Electromobility shift assays (EMSA) showed that inhibition of MSK1 resulted in a marked reduction in NF-κB binding affinity without altering the nuclear translocation of NF-κB. Supershift EMSA implicate that the p65, but not p50, isoform of NF-κB is involved in late-phase activation in response to UVB irradiation. Together, the results of these studies shed light onto a novel pathway of MSK1-mediated late-phase activation of NF-κB in response to UVB irradiation.

  13. Binding of NF-κB to nucleosomes: effect of translational positioning, nucleosome remodeling and linker histone H1.

    Directory of Open Access Journals (Sweden)

    Imtiaz Nisar Lone

    Full Text Available NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser footprinting, hydroxyl footprinting and electrophoretic mobility shift assay to investigate the binding of NF-κB to nucleosomal templates. We show that NF-κB p50 homodimer is able to bind to its recognition sequence, when it is localized at the edge of the core particle, but not when the recognition sequence is at the interior of the nucleosome. Remodeling of the nucleosome by the chromatin remodeling machine RSC was not sufficient to allow binding of NF-κB to its recognition sequence located in the vicinity of the nucleosome dyad, but RSC-induced histone octamer sliding allowed clearly detectable binding of NF-κB with the slid particle. Importantly, nucleosome dilution-driven removal of H2A-H2B dimer led to complete accessibility of the site located close to the dyad to NF-κB. Finally, we found that NF-κB was able to displace histone H1 and prevent its binding to nucleosome. These data provide important insight on the role of chromatin structure in the regulation of transcription of NF-κB dependent genes.

  14. Characterization of NF-kappa B/I kappa B proteins in zebra fish and their involvement in notochord development.

    Science.gov (United States)

    Correa, Ricardo G; Tergaonkar, Vinay; Ng, Jennifer K; Dubova, Ilir; Izpisua-Belmonte, Juan Carlos; Verma, Inder M

    2004-06-01

    Although largely involved in innate and adaptive immunity, NF-kappa B plays an important role in vertebrate development. In chicks, the inactivation of the NF-kappa B pathway induces functional alterations of the apical ectodermal ridge, which mediates limb outgrowth. In mice, the complete absence of NF-kappa B activity leads to prenatal death and neural tube defects. Here, we report the cloning and characterization of NF-kappa B/I kappa B proteins in zebra fish. Despite being ubiquitously expressed among the embryonic tissues, NF-kappa B/I kappa B members present distinct patterns of gene expression during the early zebra fish development. Biochemical assays indicate that zebra fish NF-kappa B proteins are able to bind consensus DNA-binding (kappa B) sites and inhibitory I kappa B alpha proteins from mammals. We show that zebra fish I kappa B alphas are degraded in a time-dependent manner after induction of transduced murine embryo fibroblasts (MEFs) and that these proteins are able to rescue NF-kappa B activity in I kappa B alpha(-/-) MEFs. Expression of a dominant-negative form of the murine I kappa B alpha (mI kappa B alpha M), which is able to block NF-kappa B in zebra fish cells, interferes with the notochord differentiation, generating no tail (ntl)-like embryos. This phenotype can be rescued by coinjection of the T-box gene ntl (Brachyury homologue), which is typically required for the formation of posterior mesoderm and axial development, suggesting that ntl lies downstream of NF-kappa B . We further show that ntl and Brachyury promoter regions contain functional kappa B sites and NF-kappa B can directly modulate ntl expression. Our study illustrates the conservation and compatibility of NF-kappa B/I kappa B proteins among vertebrates and the importance of NF-kappa B pathway in mesoderm formation during early embryogenesis.

  15. Understand and Challenge of ISO 26000%ISO26000的理解和应对

    Institute of Scientific and Technical Information of China (English)

    吴士权

    2011-01-01

    2010年11月1日,国际标准化组织(ISO)在瑞士日内瓦国际会议中心举办了题为“共担责任,实现可持续发展”的《ISO 26000社会责任指南》标准发布会,隆重宣布即日起正式颁布和实施ISO 26000《社会责任指南》国际标准.作为ISO将标准制定视角从传统的工程技术领域转向国际社会政治经济和伦理道德领域的里程碑性标准,该标准的诞生意义重大.

  16. Metrological management evaluation based on ISO10012: an empirical study in ISO-14001-certified Spanish companies

    Energy Technology Data Exchange (ETDEWEB)

    Beltran, Jaime; Rivas, Miguel [Andalusian Institute of Technology, C/Leonardo Da Vinci, 2, 41092 Sevilla (Spain); Munuzuri, Jesus [University of Seville, Av/Americo Vespucio, s/n. 41092 Sevilla (Spain); Gonzalez, Cristina [National University of Distance Education, C/Juan del Rosal, 2, 28040 Madrid (Spain)

    2010-01-15

    Environmental management systems based on the ISO 14001 standard rely strongly on metrological measurement and confirmation processes to certify the extent to which organizations monitor and improve their environmental behavior. Nevertheless, the literature lacks in studies that assess the influence of these metrological processes on the performance of environmental management in organizations, even now that the international standard ISO 10012 is already available to establish requisites and guidelines for the development of a metrological management system that is compatible with any other standardized management system. This work seeks to assess that influence through the development of an evaluation model for metrological management, which is then validated through an experimental analysis of the results obtained from the application of an audit process in 11 Spanish companies, all ISO-14001-certified and operating in different industrial sectors. (author)

  17. NF-κB Mediates the Expression of TBX15 in Cancer Cells

    Science.gov (United States)

    Arribas, Jéssica; Cajuso, Tatiana; Rodio, Angela; Marcos, Ricard; Leonardi, Antonio; Velázquez, Antonia

    2016-01-01

    TBX15 is a T-box transcription factor essential for development, also proposed as a marker in prostate cancer; and, recently, its antiapoptotic function indicates a role in carcinogenesis. Regulation of TBX15 is uncovered. In this study, we investigated the regulation of TBX15 expression in human cancer cells, by analyzing the regulatory function of a 5’-distal conserved region of TBX15. Bisulfite sequencing showed high methylation of the CpG island contained in this region that was not correlated with TBX15 mRNA levels, in the cancer cell lines analyzed; however, after 5-aza-dC treatment of TPC-1 cells an increase of TBX15 expression was observed. We also found a significant response of TBX15 to TNF-α activation of the NF-κB pathway using five cancer cell lines, and similar results were obtained when NF-κB was activated with PMA/ionomycin. Next, by luciferase reporter assays, we identified the TBX15 regulatory region containing two functional NF-κB binding sites with response to NF-κBp65, mapping on the -3302 and -3059 positions of the TBX15 gene. Moreover, a direct interaction of NF-κBp65 with one of the two NF-κB binding sites was indicated by ChIP assays. In summary, we provide novel data showing that NF-κB signaling up-regulates TBX15 expression in cancer cells. Furthermore, the link between TBX15 and NF-κB found in this study may be important to understand cancer and development processes. PMID:27327083

  18. At the crossroads of SUMO and NF-κB

    Directory of Open Access Journals (Sweden)

    Schmidt Christian

    2003-11-01

    Full Text Available Abstract Background Recognition of pathogens by immune receptors leads to activation of macrophages, dendritic cells, and lymphocytes. Signals are communicated to enhance expression of target molecules such as cytokines and adhesion molecules, depending on activation of various inducible transcription factors, among which the family NF-κB transcription factors plays an evolutionarily conserved and critical role. Classical activation of NF-κB involves phosphorylation, polyubiquitination and subsequent degradation of the inhibitor molecules of NF-κB, referred to as IκB. Modification of IκBα, one of the mammalian IκB isoforms, with the small ubiquitin-like modifier (SUMO results its protection from degradation. Presentation of the hypothesis SUMO-IκBα localizes in the nucleus. The nuclear SUMO-IκBα pool may be dynamic. SUMO-IκBα functions as synergy control factor. Testing the hypothesis Immunoprecipitation from cellular fractions, 35S methionine pulse-chase, and FRET assays should reveal the localization of SUMO-IκBα and the dynamics of the pool. Expression of SUMOylation defective IκBα in an IκBα -/- background should yield insights into the function of SUMO-IκBα. Implication of the hypothesis IκBα contains the required SUMOylation motif but IκBβ does not. The suggested study would provide evidence whether or not IκBα and IκBβ can substitute each other. In addition, the suggested assays would reveal a possible redundancy in controlling transcriptional activity of NF-κB.

  19. Neuronal RING finger protein 11 (RNF11 regulates canonical NF-κB signaling

    Directory of Open Access Journals (Sweden)

    Pranski Elaine L

    2012-04-01

    Full Text Available Abstract Background The RING domain-containing protein RING finger protein 11 (RNF11 is a member of the A20 ubiquitin-editing protein complex and modulates peripheral NF-κB signaling. RNF11 is robustly expressed in neurons and colocalizes with a population of α-synuclein-positive Lewy bodies and neurites in Parkinson disease patients. The NF-κB pathway has an important role in the vertebrate nervous system, where the absence of NF-κB activity during development can result in learning and memory deficits, whereas chronic NF-κB activation is associated with persistent neuroinflammation. We examined the functional role of RNF11 with respect to canonical NF-κB signaling in neurons to gain understanding of the tight association of inflammatory pathways, including NF-κB, with the pathogenesis of neurodegenerative diseases. Methods and results Luciferase assays were employed to assess NF-κB activity under targeted short hairpin RNA (shRNA knockdown of RNF11 in human neuroblastoma cells and murine primary neurons, which suggested that RNF11 acts as a negative regulator of canonical neuronal NF-κB signaling. These results were further supported by analyses of p65 translocation to the nucleus following depletion of RNF11. Coimmunoprecipitation experiments indicated that RNF11 associates with members of the A20 ubiquitin-editing protein complex in neurons. Site-directed mutagenesis of the myristoylation domain, which is necessary for endosomal targeting of RNF11, altered the impact of RNF11 on NF-κB signaling and abrogated RNF11’s association with the A20 ubiquitin-editing protein complex. A partial effect on canonical NF-κB signaling and an association with the A20 ubiquitin-editing protein complex was observed with mutagenesis of the PPxY motif, a proline-rich region involved in Nedd4-like protein interactions. Last, shRNA-mediated reduction of RNF11 in neurons and neuronal cell lines elevated levels of monocyte chemoattractant protein 1 and

  20. Lean principles adoption in environmental management system (EMS - ISO 14001

    Directory of Open Access Journals (Sweden)

    Perumal Puvanasvaran

    2012-12-01

    Full Text Available Purpose: The purpose of this study is to examine the characteristic of the lean principles into ISO 14001 and to propose linkage of the lean principles and ISO 14001.Design/methodology/approach: To achieve the objective of the study, literature survey and quantitative research method using questionnaires survey are used.Findings and Originality/value: The findings of this study confirm that ISO 14001 certified company adopted lean production practices.  The study also proves that lean principles have positive and significant relationship with ISO 14001 EMS and the linkage can be made between lean principles and ISO 14001 to achieve Continual Improvement.Research limitations/implications: The small sizes of the sample of the participating companies are the main limitations of this study and this research mainly focuses on the manufacturing environment and services industry.Practical implications: This research show that all ISO 14001 companies do adopt at least one lean production practices and the main findings are lean principles has positive and highly significant relationship with ISO 14001 requirements.  This is because the integration of lean principles into ISO 14001 will serve practical methods for ISO14001 EMS to achieve the continual improvement.Originality/value: This research is amongst the first to study the combined lean principles with ISO 1400.  Based on the current situation, there is no integration within this two management system. 

  1. A graded approach to ISO 9000 implementation for records managers

    Energy Technology Data Exchange (ETDEWEB)

    Pasterczyk, C.E.

    1994-08-01

    ISO 9000 is a series of quality system standards developed by Technical Committee 176 of the International Standards Organization. The series includes three standards, ISO 9001 `Quality systems--Model for quality assurance in design/development, production, installation and serving,` ISO 9002 `Quality systems--Model for quality assurance in production and installation,` ISO 9003 `Quality systems--Model for quality assurance in final inspection and test,` and two guidelines, ISO 9000 `Quality management and quality assurance standards` and ISO 9004 `Quality management and quality system elements--Guidelines.` In companies with quality programs already in place, the structure of the ISO series lends itself to augmenting those policies, procedures, and instructions where problems exist or where the risks of failure are greater. The objectives of this paper are to provide a basic familiarity to ISO 9000 and quality assurance concepts; provide a higher level of familiarity to the records management and document control portions of the ISO 9000 series; and to discuss a graded approach to meeting the intent of ISO 9000.

  2. ISO 639-1 and ISO 639-2: International Standards for Language Codes. ISO 15924: International Standard for Names of Scripts.

    Science.gov (United States)

    Byrum, John D.

    This paper describes two international standards for the representation of the names of languages. The first (ISO 639-1), published in 1988, provides two-letter codes for 136 languages and was produced primarily to meet terminological needs. The second (ISO 639-2) appeared in late 1998 and includes three-letter codes for 460 languages. This list…

  3. Functional analysis of splicing mutations in exon 7 of NF1 gene

    Directory of Open Access Journals (Sweden)

    Calvieri Stefano

    2007-02-01

    Full Text Available Abstract Background Neurofibromatosis type 1 is one of the most common autosomal dominant disorders, affecting about 1:3,500 individuals. NF1 exon 7 displays weakly defined exon-intron boundaries, and is particularly prone to missplicing. Methods In this study we investigated the expression of exon 7 transcripts using bioinformatic identification of splicing regulatory sequences, and functional minigene analysis of four sequence changes [c.910C>T (R304X, c.945G>A/c.946C>A (Q315Q/L316M, c.1005T>C (N335N] identified in exon 7 of three different NF1 patients. Results Our results detected the presence of three exonic splicing enhancers (ESEs and one putative exonic splicing silencer (ESS element. The wild type minigene assay resulted in three alternative isoforms, including a transcript lacking NF1 exon 7 (NF1ΔE7. Both the wild type and the mutated constructs shared NF1ΔE7 in addition to the complete messenger, but displayed a different ratio between the two transcripts. In the presence of R304X and Q315Q/L316M mutations, the relative proportion between the different isoforms is shifted toward the expression of NF1ΔE7, while in the presence of N335N variant, the NF1ΔE7 expression is abolished. Conclusion In conclusion, it appears mandatory to investigate the role of each nucleotide change within the NF1 coding sequence, since a significant proportion of NF1 exon 7 mutations affects pre-mRNA splicing, by disrupting exonic splicing motifs and modifying the delicate balance between aberrantly and correctly spliced transcripts.

  4. Enzyme assays.

    Science.gov (United States)

    Reymond, Jean-Louis; Fluxà, Viviana S; Maillard, Noélie

    2009-01-07

    Enzyme assays are analytical tools to visualize enzyme activities. In recent years a large variety of enzyme assays have been developed to assist the discovery and optimization of industrial enzymes, in particular for "white biotechnology" where selective enzymes are used with great success for economically viable, mild and environmentally benign production processes. The present article highlights the aspects of fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting, which are our particular areas of interest.

  5. Norma ISO 690:2010 [Doctorado. 2015

    OpenAIRE

    Biblioteca Universitaria

    2015-01-01

    Mediante el estudio de este tema aprenderás a gestionar adecuadamente tu bibliografía adquiriendo una serie de competencias que te permitirán: - Actuar de manera responsable, ética y legal en el uso de la información empleada para realizar un trabajo académico. - Identificar el estilo de cita más adecuado para cada disciplina científica. - Identificar correctamente y elaborar las referencias bibliográficas correspondientes a distintos tipos de documentos, de acuerdo con la norma ISO 690:2010....

  6. Norma ISO 690:2010 [Doctorado. 2014

    OpenAIRE

    Biblioteca Universitaria

    2014-01-01

    Mediante el estudio de este tema aprenderás a gestionar adecuadamente tu bibliografía adquiriendo una serie de competencias que te permitirán: - Actuar de manera responsable, ética y legal en el uso de la información empleada para realizar un trabajo académico. - Identificar el estilo de cita más adecuado para cada disciplina científica. - Identificar correctamente y elaborar las referencias bibliográficas correspondientes a distintos tipos de documentos, de acuerdo con la norma ISO 690:2010....

  7. Elevated Risk for MPNST in NF1 Microdeletion Patients

    OpenAIRE

    De Raedt, T.; Brems, H.; Wolkenstein, P.; Vidaud, D; Pilotti, S; Perrone, F.; Mautner, V; Frahm, S.; Sciot, R; Legius, E

    2003-01-01

    An NF1 microdeletion is the single most commonly reported mutation in individuals with neurofibromatosis type 1 (NF1). Individuals with an NF1 microdeletion have, as a group, more neurofibromas at a younger age than the group of all individuals with NF1. We report that NF1 microdeletion individuals additionally have a substantially higher lifetime risk for the development of malignant peripheral nerve sheath tumors than individuals with NF1 who do not have an NF1 microdeletion. This should be...

  8. Desflurane preconditioning induces oscillation of NF-κB in human umbilical vein endothelial cells.

    Directory of Open Access Journals (Sweden)

    Juan Yi

    Full Text Available BACKGROUND: Nuclear factor kappa B (NF-κB has been implicated in anesthetic preconditioning (APC induced protection against anoxia and reoxygenation (A/R injury. The authors hypothesized that desflurane preconditioning would induce NF-κB oscillation and prevent endothelial cells apoptosis. METHODS: A human umbilical vein endothelial cells (HUVECs A/R injury model was used. A 30 minute desflurane treatment was initiated before anoxia. NF-κB inhibitor BAY11-7082 was administered in some experiments before desflurane preconditioning. Cells apoptosis was analyzed by flow cytometry using annexin V-fluorescein isothiocyanate staining and cell viability was evaluated by modified tertrozalium salt (MTT assay. The cellular superoxide dismutases (SOD activitiy were tested by water-soluble tetrazolium salt (WST-1 assay. NF-κB p65 subunit nuclear translocation was detected by immunofluorescence staining. Expression of inhibitor of NF-κB-α (IκBα, NF-κB p65 and cellular inhibitor of apoptosis 1 (c-IAP1, B-cell leukemia/lymphoma 2 (Bcl-2, cysteine containing aspartate specific protease 3 (caspases-3 and second mitochondrial-derived activator of caspase (SMAC/DIABLO were determined by western blot. RESULTS: Desflurane preconditioning caused phosphorylation and nuclear translocation of NF-κB before anoxia, on the contrary, induced the synthesis of IκBα and inhibition of NF-κB after reoxygenation. Desflurane preconditioning up-regulated the expression of c-IAP1 and Bcl-2, blocked the cleavage of caspase-3 and reduced SMAC release, and decreased the cell death of HUVECs after A/R. The protective effect was abolished by BAY11-7082 administered before desflurane. CONCLUSIONS: The results demonstrated that desflurane activated NF-κB during the preconditioning period and inhibited excessive activation of NF-κB in reperfusion. And the oscillation of NF-κB induced by desflurane preconditioning finally up-regulated antiapoptotic proteins expression and

  9. NORSOK/ISO and underwater systems; ISO subsea standards program 1

    Energy Technology Data Exchange (ETDEWEB)

    Inderberg, Olav

    1999-07-01

    This presentation discusses the status for the NORSOK work, the experience from the development of standards, the experience from operation with NORSOK, the Petroleum Directorate's recommendations and Norwegian NORSOK/ISO standards, and the globalization of Norwegian petroleum industry.

  10. Translation initiation factor (iso) 4E interacts with BTF3, the beta subunit of the nascent polypeptide-associated complex.

    Science.gov (United States)

    Freire, Miguel Angel

    2005-01-31

    A two-hybrid screen with the translation initiation factor, eIF(iso)4E from Arabidopsis, identified a clone encoding a lipoxygenase type 2 [Freire, M.A., et al., 2000. Plant lipoxygenase 2 is a translation initiation factor-4E-binding protein. Plant Molecular Biology 44, 129-140], and three cDNA clones encoding the homologue of the mammalian BTF3 factor, the beta subunit of the nascent polypeptide-associated complex (NAC). Here we report on the interaction between the translation initiation factor eIF(iso)4E and AtBTF3. AtBTF3 protein is able to interact with the wheat initiation factors eIF4E and eIF(iso)4E. AtBTF3 contains a sequence related to the prototypic motif found on most of the 4E-binding proteins, and competes with the translation initiation factor eIF(iso)4G for eIF4(iso)4E binding, in a two hybrid interference assay. These findings provide a molecular link between the translation initiation mechanism and the emergence of the nascent polypeptide chains.

  11. Cotation de fabrication avec les normes ISO

    CERN Document Server

    Anselmetti, Bernard

    2010-01-01

    La cotation ISO en production impose de nouvelles méthodologies pour élaborer les documents de maîtrise de la qualité géométrique des produits : les dessins de phase, du brut et d'états intermédiaires pour la conformité des pièces et les fiches de production, de réglage, de contrôle et de surveillance pour la conformité des moyens de production. Une méthode de transfert unidirectionnelle simple permet d'établir des dessins de phase en normes ISO et d'optimiser la répartition des tolérances à isocapabilité. Cette cotation est déclinée en cotation d'état intermédiaire, de production et de réglage. Une méthode tridimensionnelle est proposée pour prendre en compte les effets des défauts angulaires. Une maquette montre les fonctionnalités envisageables pour les futurs outils d'aides au tolérancement de fabrication au sein de la CFAO. De nombreux exercices illustrent les différentes démarches. Ce Manuel de tolérancement est principalement destiné aux préparateurs méthodes et aux c...

  12. Experience with ISO quality control in assisted reproductive technology.

    Science.gov (United States)

    Alper, Michael M

    2013-12-01

    Assisted reproductive technology (ART) programs are complex organizations requiring the integration of multiple disciplines. ISO 9001:2008 is a quality management system that is readily adaptable to an ART program. The value that ISO brings to the entire organization includes control of documents, clear delineation of responsibilities of staff members, documentation of the numerous processes and procedures, improvement in tracking and reducing errors, and overall better control of systems. A quality ART program sets quality objectives and monitors their progress. ISO provides a sense of transparency within the organization and clearer understanding of how service is provided to patients. Most importantly, ISO provides the framework to allow for continual improvement.

  13. 实施CMMI企业的ISO9000审核%ISO9000 Audit on the CMMI Implementation Company

    Institute of Scientific and Technical Information of China (English)

    齐元

    2011-01-01

    该文结合CMMI模型的特点和企业实施CMMI的经验,分别从开发活动和项目管理问题的角度说明针对实施CMMI企业开展ISO9000审核时需要关注的重点,以供ISO9000审核人员以及期望融合CMMI以及ISO9000体系的企业管理人员参考。%This paper is to discuss the key audit points of ISO9000 audit on the companies which use CMMI for process improvement.It covers development activities and project management issues and can be used as reference for ISO auditors and enterprise management who expects to integrate CMMI and ISO9000 together.

  14. Curcumin Sensitizes Hepatocellular Carcinoma Cells to Radiation via Suppression of Radiation-Induced NF-κB Activity

    Directory of Open Access Journals (Sweden)

    Fei-Ting Hsu

    2015-01-01

    Full Text Available The effects and possible underlying mechanism of curcumin combined with radiation in human hepatocellular carcinoma (HCC cells in vitro were evaluated. The effects of curcumin, radiation, and combination of both on cell viability, apoptosis, NF-κB activation, and expressions of NF-κB downstream effector proteins were investigated with 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT, NF-κB reporter gene, mitochondrial membrane potential (MMP, electrophoretic mobility shift (EMSA, and Western blot assays in Huh7-NF-κB-luc2, Hep3B, and HepG2 cells. Effect of I kappa B alpha mutant (IκBαM vector, a specific inhibitor of NF-κB activation, on radiation-induced loss of MMP was also evaluated. Results show that curcumin not only significantly enhances radiation-induced cytotoxicity and depletion of MMP but inhibits radiation-induced NF-κB activity and expressions of NF-κB downstream proteins in HCC cells. IκBαM vector also shows similar effects. In conclusion, we suggest that curcumin augments anticancer effects of radiation via the suppression of NF-κB activation.

  15. 从实践看ISO 14000对ISO 9000体系的兼容性

    Institute of Scientific and Technical Information of China (English)

    郭士毅

    2001-01-01

    本文介绍了ISO 14000系列标准在我国和日本实施的一些情况,并通过实施质量体系认证、环境体系认证事例,论述了社会、企业在环境、质量推进工作中ISO 14000对ISO 9000体系的兼容性.

  16. 浅谈ISO 14000环境管理体系标准%ISO 14000, Environment Management System Standard

    Institute of Scientific and Technical Information of China (English)

    周松波

    2002-01-01

    简要介绍了ISO 14000环境管理体系标准的核心内容,同时分别就ISO 14000与ISO 9000和清洁生产的关系进行了深入浅出的阐释,最后结合行业实际分析了机械工业制造过程中常见的与ISO 14000相关联的环境因素.

  17. Inhibition of NF-IL6 activity by manassantin B, a dilignan isolated from Saururus chinensis, in phorbol myristate acetate-stimulated U937 promonocytic cells.

    Science.gov (United States)

    Son, Kyung-No; Song, In-sung; Shin, Yong-Hyun; Pai, Tong-Kun; Chung, Dae-Kyun; Baek, Nam-In; Lee, Jung Joon; Kim, Jiyoung

    2005-08-31

    Mannasantin B, a dilignan structurally related to manssantin A, is an inhibitor of NF-kappaB transactivation. In the present study, we found that it inhibited PMA-induced expression of IL-1beta, IL-1beta mRNA, and IL-1beta promoter activity in U937 cells with IC50 values of about 50 nM. It also inhibited NF-IL6- and NF-kappaB-induced activation of IL-1beta, with IC50 values of 78 nM and 1.6 microM, respectively, revealing a potent inhibitory effect on NF-IL6. Electrophoretic mobility shift assays showed that manassantin B had an inhibitory effect on DNA binding by NF-IL6, but not by NF-kappaB. Further analysis revealed that transactivation by NF-IL6 was also inhibited. Our results indicate that manassantin B suppresses expression of IL-1beta in promonocytic cells by inhibiting not only NF-kappaB but also NF-IL6 activity. Furthermore, our observations suggest that manassantin B may be clinically useful as a potent inhibitor of NF-IL6 activity.

  18. Iso 19011:2002--a combined auditing standard for quality and environmental management systems.

    Science.gov (United States)

    Johnson, G L

    2000-01-01

    In a precedent-setting decision in 1998, the International Organization for Standardization (ISO) directed ISO Technical Committee (TC) 176 on Quality Management and ISO TC 207 on Environmental Management to develop jointly a single guideline standard for auditing quality and environmental management systems. When approved, this standard would replace ISO 10011-1, ISO 10011-2, and ISO 10011-3 on quality auditing and ISO 14010, ISO 14011, and ISO 14012 on environmental auditing. A Joint Working Group (JWG) was established comprising experts from both TC 176 and TC 207 to develop the new standard, ISO 19011, Guidelines on Quality and/or Environmental Management Systems Auditing, and to incorporate lessons learned from efforts to improve compatibility between ISO 9001/9004 and ISO 14001/14004, the standards for quality and environmental management systems, respectively. Work is proceeding on the development of ISO 19011 with an expected completion in early 2002.

  19. Immunohistochemical NF1 analysis does not predict NF1 gene mutation status in pheochromocytoma.

    Science.gov (United States)

    Stenman, Adam; Svahn, Fredrika; Welander, Jenny; Gustavson, Boel; Söderkvist, Peter; Gimm, Oliver; Juhlin, C Christofer

    2015-03-01

    Pheochromocytomas (PCCs) are tumors originating from the adrenal medulla displaying a diverse genetic background. While most PCCs are sporadic, about 40 % of the tumors have been associated with constitutional mutations in one of at least 14 known susceptibility genes. As 25 % of sporadic PCCs harbor somatic neurofibromin 1 gene (NF1) mutations, NF1 has been established as the most recurrently mutated gene in PCCs. To be able to pinpoint NF1-related pheochromocytoma (PCC) disease in clinical practice could facilitate the detection of familial cases, but the large size of the NF1 gene makes standard DNA sequencing methods cumbersome. The aim of this study was to examine whether mutations in the NF1 gene could be predicted by immunohistochemistry as a method to identify cases for further genetic characterization. Sixty-seven PCCs obtained from 67 unselected patients for which the somatic and constitutional mutational status of NF1 was known (49 NF1 wild type, 18 NF1 mutated) were investigated for NF1 protein immunoreactivity, and the results were correlated to clinical and genetic data. NF1 immunoreactivity was absent in the majority of the PCCs (44/67; 66 %), including 13 out of 18 cases (72 %) with a somatic or constitutional NF1 mutation. However, only a minority of the NF1 wild-type PCCs (18/49; 37 %) displayed retained NF1 immunoreactivity, thereby diminishing the specificity of the method. We conclude that NF1 immunohistochemistry alone is not a sufficient method to distinguish between NF1-mutated and non-mutated PCCs. In the clinical context, genetic screening therefore remains the most reliable tool to detect NF1-mutated PCCs.

  20. Mednarodni standardi - veličine in enote (ISO 31-0 do 31-13)

    OpenAIRE

    Glavič, Peter

    2015-01-01

    In this paper the international standards ISO 31 (Quantities and units) are presented with the following parts: ISO 31-0 (General principles), ISO 31-1 (Space and time), ISO 31-2 (Periodic and related phenomena), ISO 31-3 (Mechanics), ISO 31-4 (Heat), ISO 31-5 (Electricity and magnetism), ISO 31-8 (Physical chemistry and molecular physics), ISO 31-12 (Characteristic numbers)and others. The emphasis is given on the basic principles, which is important for writing of reports, presentations, art...

  1. ISO proves that intergalactic space is dusty

    Science.gov (United States)

    1997-11-01

    In the past, astronomers have considered intergalactic space to be extremely clean. Except in the vast concentrations of stars, gas and dust that make up the galaxies themselves, the Universe was supposed to be filled only by very thin traces of invisible gas. ISO's detection of dust means that the Universe is less transparent than astronomers have assumed. Their cosmic window-pane is slightly dirty and large-scale inferences based on the brightnesses of distant galaxies and quasars may be affected. Emissions from the intergalactic dust were picked up by the photometer ISOPHOT. A team of German, British, Spanish and Danish astronomers contributed this versatile set of detectors to ISO. The leader of the ISOPHOT team is Dietrich Lemke of the Max-Planck Institut fr Astronomie (MPIA) in Heidelberg, Germany. "ISOPHOT in ISO is the only instrument in existence capable of making this detection" Lemke says. "The intergalactic dust is so cold that we need a very cold telescope to detect it. The strongest emissions from the dust are at a wavelength of 0.1-0.2 millimetre, which cannot be well observed from the Earth. ISO provides telescope in space cooled by superfluid helium to within 2 degrees of absolute zero. ISOPHOT is the instrument on ISO that measures infrared intensities at the longest wavelengths, up to 0.2 millimetre." ISOPHOT's advantages made finding the intergalactic dust possible, but not easy. The observations pushed instrumental sensitivity to the limit, and emissions from cold dust clouds in the Milky Way Galaxy confused the picture. The signal of intergalactic dust emerged clearly only after extensive data analysis. Cold dust in a hot cluster Our home Galaxy, the Milky Way, belongs to a very small group of galaxies. Intergalactic dust may very well be present nearby, but it is likely to be sparse and scattered. A team of astronomers, from MPIA Heidelberg and Helsinki Observatory, hoped that the intergalactic dust might be easier to recognise in a large

  2. Intermediate species measurement during iso-butanol auto-ignition

    KAUST Repository

    Ji, Weiqi

    2015-10-01

    © 2015 The Combustion Institute.Published by Elsevier Inc. All rights reserved. This work presents the time histories of intermediate species during the auto-ignition of iso-butanol at high pressure and intermediate temperature conditions obtained using a rapid compression machine and recently developed fast sampling system. Iso-butanol ignition delays were acquired for iso-butanol/O2 mixture with an inert/O2 ratio of 7.26, equivalence ratio of 0.4, in the temperature range of 840-950 K and at pressure of 25 bar. Fast sampling and gas chromatography were used to acquire and quantify the intermediate species during the ignition delay of the same mixture at P = 25.3 bar and T = 905 K. The ignition delay times and quantitative measurements of the mole fraction time histories of methane, ethene, propene, iso-butene, iso-butyraldehyde, iso-butanol, and carbon monoxide were compared with predictions from the detailed mechanisms developed by Sarathy et al., Merchant et al., and Cai et al. It is shown that while the Sarathy mechanism well predicts the overall ignition delay time, it overpredicts ethene by a factor of 6-10, underpredicts iso-butene by a factor of 2, and overpredicts iso-butyraldehyde by a factor of 2. Reaction path and sensitivity analyses were carried out to identify the reactions responsible for the observed inadequacy. The rates of iso-butanol hydrogen atom abstraction by OH radical and the beta-scission reactions of hydroxybutyl radicals were updated based on recently published quantum calculation results. Significant improvements were achieved in predicting ignition delay at high pressures (25 and 30 bar) and the species concentrations of ethene and iso-butene. However, the updated mechanism still overpredicts iso-butyraldehyde concentrations. Also, the updated mechanism degrades the prediction in ignition delay at lower pressure (15 bar) compared to the original mechanism developed by Sarathy et al.

  3. Oncogenic activation of NF-kappaB.

    Science.gov (United States)

    Staudt, Louis M

    2010-06-01

    Recent genetic evidence has established a pathogenetic role for NF-kappaB signaling in cancer. NF-kappaB signaling is engaged transiently when normal B lymphocytes respond to antigens, but lymphomas derived from these cells accumulate genetic lesions that constitutively activate NF-kappaB signaling. Many genetic aberrations in lymphomas alter CARD11, MALT1, or BCL10, which constitute a signaling complex that is intermediate between the B-cell receptor and IkappaB kinase. The activated B-cell-like subtype of diffuse large B-cell lymphoma activates NF-kappaB by a variety of mechanisms including oncogenic mutations in CARD11 and a chronic active form of B-cell receptor signaling. Normal plasma cells activate NF-kappaB in response to ligands in the bone marrow microenvironment, but their malignant counterpart, multiple myeloma, sustains a variety of genetic hits that stabilize the kinase NIK, leading to constitutive activation of the classical and alternative NF-kappaB pathways. Various oncogenic abnormalities in epithelial cancers, including mutant K-ras, engage unconventional IkappaB kinases to activate NF-kappaB. Inhibition of constitutive NF-kappaB signaling in each of these cancer types induces apoptosis, providing a rationale for the development of NF-kappaB pathway inhibitors for the treatment of cancer.

  4. Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.

    Science.gov (United States)

    Kim, Jinhee; Kang, Won-Hee; Hwang, Jeena; Yang, Hee-Bum; Dosun, Kim; Oh, Chang-Sik; Kang, Byoung-Cheorl

    2014-08-01

    The protein-protein interaction between VPg (viral protein genome-linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad-spectrum resistance in Chinese cabbage (Brassica rapa) to Turnip mosaic virus (TuMV), which is one of the most important potyviruses, by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and B. rapa eIF(iso)4E. The seven amino acids in the cap-binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap-binding pockets, and mutated. Yeast two-hybrid assay and co-immunoprecipitation analysis demonstrated that W95L, K150L and W95L/K150E amino acid mutations of B. rapa eIF(iso)4E interrupted its interaction with TuMV VPg. All eIF(iso)4E mutants were able to complement an eIF4E-knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L, W95L/K150E and eIF(iso)4E wild-type were over-expressed in a susceptible Chinese cabbage cultivar. Evaluation of the TuMV resistance of T1 and T2 transformants demonstrated that the over-expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge-based approaches for the engineering of broad-spectrum resistance in Chinese cabbage.

  5. Lipopolysaccharide enhances the inhibition of NF-κB expression in NNK-mediated peritoneal macrophages

    Institute of Scientific and Technical Information of China (English)

    Bin Li; Mei Wu; Xiaoping Liu

    2014-01-01

    The aim of the study was to investigate the efect of lipopolysaccharide (LPS) on the expression of nuclear factor kappa B (NF-κB) in 4-(methylitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-mediated primary mouse peritoneal macrophagesin vitro.Methods: The activity of peritoneal macrophages treated with diferent concentrations of LPS was de-tected by MTT assay in rider to find the optimal concentration. Peritoneal macrophages were also treated with NNK (100-500μM), with or without LPS for 9 h. The expression of NF-κB was demonstrated via immunocytochemistry (ICC) and Western-blot, respectively.Results:The concentration of LPS at 25 μg/mL was found to be the optimal concentration to improve the activity of peritoneal macrophages (P < 0.01). Simultaneously, LPS (25 μg/mL) increased the expression of NF-κB in both the nucleus and cytoplasm and facilitated transfer of NF-κB to the nucleus. NNK treatment significantly inhibited the expression of NF-κB in a concentration-dependent manner, among the LPS-stimulated or unstimulated peritoneal macrophages, espe-cialy when cotreated with LPS (25 μg/mL,P < 0.01 ). Furthermore, NNK treatment (500 μM) with LPS yielded a significant decrease in NF-κB translocation to nucleus and inhibited the expression of NF-κB (P < 0.005).Conclusion: LPS enhances the suppression of NF-κB expression in NNK-mediated mouse peritoneal macrophages, which may provide a theoretical basis for the inhibition of cancer.

  6. Elevated expression of NF-kappaB in oral submucous fibrosis--evidence for NF-kappaB induction by safrole in human buccal mucosal fibroblasts.

    Science.gov (United States)

    Ni, Wei-Feng; Tsai, Chung-Hung; Yang, Shun-Fa; Chang, Yu-Chao

    2007-07-01

    Nuclear factor-kappa B (NF-kappaB) is considered to be important in many inflammatory and immune responses. The aim of this study was to compare NF-kappaB expression in normal human buccal mucosa and oral submucous fibrosis (OSF) specimens and further explore the potential mechanism that may lead to induction of NF-kappaB expression. Seventeen OSF and six normal buccal mucosa specimens were examined by immunohistochemistry. Primary human buccal mucosal fibroblasts (BMFs) were established and challenged with safrole, a major polyphenolic compound in the influorescence of Piper betel, by cytotoxicity and western blot assays. Furthermore, glutathione precursor N-acetyl-L-cysteine (NAC), extracellular signal-regulated protein kinase (ERK) inhibitor PD98059, cyclooxygenase-2 (COX-2) inhibitor NS-398, dexamethasone, and cyclosporin A were added to find the possible mechanism. NF-kappaB expression was significantly higher in OSF specimens and expressed mainly by fibroblasts, endothelial cells, and inflammatory cells. Safrole was cytotoxic to BMFs in a dose-dependent manner (psafrole (psafrole induced-NF-kappaB expression (psafrole in fibroblasts may be mediated by ERK activation and COX-2 signal transduction pathway.

  7. ISO Images of Starbursts and Active Galaxies

    CERN Document Server

    Mirabel, I F

    1999-01-01

    We present some highlights from the mid-infrared (5-16 micron) images of mergers of massive galaxies obtained with the Infrared Space Observatory (ISO). We have observed: 1) ultraluminous infrared nuclei, 2) luminous dust-enshrouded extranuclear starbursts, and 3) active galaxy nuclei (AGNs). In this contribution we discuss the observations of Arp 299, a prototype for very luminous infrared galaxies, the Antennae which is a prototype of mergers, and Centaurus A which is the closest AGN to Earth. From these observations we conclude the following: 1) the most intense starbursts in colliding systems of galaxies and the most massive stars are dust-enshrouded in regions that appear inconspicuous at optical wavelengths, 2) the most intense nuclear infrared sources are a combination of AGN and starburst activity, 3) the hosts of radio loud AGNs that trigger giant double-lobe structures may be symbiotic galaxies composed of barred spirals inside ellipticals.

  8. Transplacental haemorrhage and maternal iso-immunization

    Directory of Open Access Journals (Sweden)

    Mehta Daxa

    1979-01-01

    Full Text Available Foetomaternal haemorrhage is one of the important factors influencing maternal iso-immunization. In the present report at-tempt is made to study various aspects of foetomaternal haemor-rhage. While the overall incidence of foetal cell leak was observed in 21.43%, the incidence in the complicated deliveries ranged from. 27.94 to 85.72%. Incidence was not influenced by parity, however ABO compatible deliveries showed slight increase in the incidence (22.56% as compared to ABO incompatible (18.75% deliveries. ABO incompatible foetal cells were eliminated within 24 hours after delivery. Incidence of foetal cell leak was 8.28% in medical termination of pregnancy. Anth-Rh was produced in two out of nine women having foetal cell leak.

  9. Iso-electric point of fluid

    CERN Document Server

    Wan, Li

    2016-01-01

    Iso-electric point(IEP) is the PH, at which the $\\zeta$ potential is measured to be zero. The occurrence of IEP has been understood due to the neutralization of surface charge density (SCD) at the solid-liquid interface. In this work, we use the potential trap model to study the sources of the surface charge density at verious PC and PH, by taking the water-silica system as an example. It is revealed that in the case of $PH9$. Due to the mass action law, the dissociation of water molecules is suppressed at the PH close to IEP, leading to a zero surface charge density. In this way, zero $\\zeta$ potential is obtained at the IEP. It has also been obtained that the increase of the salt concentration in the water can decrease the $\\zeta$ potential, but increase the surface charge density.

  10. 聚焦ISO 9001:2015

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    本文发表于2014年9月的《质量进展》,作者桑福德•雷比斯曼(Sandford Liebesman)是美国新泽西桑福德质量咨询公司总裁,拥有30余年的质量领域从业经验,先后供职于贝尔实验室、朗讯科技和贝尔通信研究所。作为美国质量协会的会员,曾担任电子与通信委员会主席一职。从1984年至今,他一直是国际标准化组织质量管理和质量保证技术委员会美国技术顾问小组的成员。在此期间,他参与了所有版本的标准修订工作。ISO 9001新版标准首次引入了“风险”概念,本文通过对ISO9001相关风险条款的详细分析,说明如何增强企业的风险管理。包括风险的4种分类以及风险的分析方法。

  11. Manufacturing Bms/Iso System Review

    Science.gov (United States)

    Gomez, Yazmin

    2004-01-01

    The Quality Management System (QMS) is one that recognizes the need to continuously change and improve an organization s products and services as determined by system feedback, and corresponding management decisions. The purpose of a Quality Management System is to minimize quality variability of an organization's products and services. The optimal Quality Management System balances the need for an organization to maintain flexibility in the products and services it provides with the need for providing the appropriate level of discipline and control over the processes used to provide them. The goal of a Quality Management System is to ensure the quality of the products and services while consistently (through minimizing quality variability) meeting or exceeding customer expectations. The GRC Business Management System (BMS) is the foundation of the Center's ISO 9001:2000 registered quality system. ISO 9001 is a quality system model developed by the International Organization for Standardization. BMS supports and promote the Glenn Research Center Quality Policy and wants to ensure the customer satisfaction while also meeting quality standards. My assignment during this summer is to examine the manufacturing processes used to develop research hardware, which in most cases are one of a kind hardware, made with non conventional equipment and materials. During this process of observation I will make a determination, based on my observations of the hardware development processes the best way to meet customer requirements and at the same time achieve the GRC quality standards. The purpose of my task is to review the manufacturing processes identifying opportunities in which to optimize the efficiency of the processes and establish a plan for implementation and continuous improvement.

  12. The ISO View of Star Forming Galaxies

    Science.gov (United States)

    Helou, George

    1999-01-01

    ISO studies of normal galaxies in the local Universe have revealed basic new properties whose significant implications for the star formation process and cosmology are only starting to be understood. This review will touch on the general results of a statistical nature, and provide a quick summary of the profusion of exciting results on individual objects. In the mid-infrared, PHT-S has established that the spectra of star forming galaxies between 6 and-13microns are dominated by the Aromatic Features in Emission (AFE), and show little variation as a function of the heating intensity. The Carriers of the AFE (CAFE) are thus a universal component of dust with standard properties, and contribute between 10 and 25% of the total dust luminosity. In addition to AFE, the spectra show a low-level continuum detectable at wavelengths longer than 3.5microns whose origin is still under investigation. The mid-infrared colors formed as the ratio of flux densities in the 6.75micron and the 15micron bands of ISO-CAM remain essentially constant and near unity for quiescent and mildly active galaxies. As dust heating increases further, the 15micron flux increases steeply compared to 6.75microns, indicating that dust heated to 100Kgalaxy become more active in star formation, its [CII] flux weakens relative to total dust emission while the [OI] does not. This behavior has attracted much interest because it extrapolates to the most active galaxies, making them weaker in [CII] than previously expected. Several explanations for the effect have been advanced, and will be discussed in this review. Spectroscopy with SWS has measured molecular hydrogen in galaxies, providing a powerful handle on the warm molecular gas content. SWS and CAM-CVF studies targeting ionic fine-structure lines have demonstrated their value as diagnostics of the radiation field.

  13. Revision of the ISO and EN radiation sterilization standards

    DEFF Research Database (Denmark)

    Miller, A.; Hansen, J.

    2002-01-01

    The radiation sterilization standards, ISO 11137 and EN 552, are now being revised under "ISO lead", with the aim of producing only one international standard, although in four parts: (1) requirements. (2) dose-setting methods, (3) dose-substantiation methods and (4) dosimetry. Several aspects...

  14. Evaluating Education Quality in Terms of ISO9000 Standards

    Science.gov (United States)

    Fengchun, Cheng; Vogel, Linda R.; Zhaoyu, Zheng

    2014-01-01

    While nations measure the quality of educational systems by different standards, many nations, such as China, have adopted the ISO9000 quality standards to assess the quality of schools. This article explores the various elements of the the ISO9000 standards and how each element relates to the various groups that are consumers or stakeholders in…

  15. ISO 14001 ENVIRONMENTL MANAGEMENT SYSTEMS IN GOVERNMENT AND INDUSTRY

    Science.gov (United States)

    Two new USEPA documents address environmental management systems (EMS) from the perspectives of government and industry and are offered as tools for understanding the process of the ISO 14000 Standards development and usefulness of an EMS approach. The first document, ISO 14001 -...

  16. A Stepwise ISO-Based TQM Implementation Approach Using ISO 9001:2015

    Directory of Open Access Journals (Sweden)

    Chen Chi-kuang

    2016-12-01

    Full Text Available The lack of an implementation roadmap always deters enterprises from choosing Total Quality Management (TQM as its major management approach. This paper proposes a stepwise ISO-based TQM implementation approach which is based on the notion of the new three-dimensional overall business excellence framework developed by Dahlgaard et al. [1]. The proposed approach consists of nine steps comprising three categories: “TQM faith building”, “TQM tools and techniques learning”, and “system development”. The steps in each of the three categories are arranged to span across the proposed nine-step approach. The ISO 9001:2015 standard is used as a case study to demonstrate the proposed approach. The ideas and benefits of the proposed approach are further discussed in relation to this illustration.

  17. Hepatocyte growth factor-induced proliferation of hepatic stem-like cells depends on activation of NF-κB

    Institute of Scientific and Technical Information of China (English)

    PengYao; YiqunZhan; WangxiangXu; ChangyanLi; PeibinYue; ChengwangXu; DarongHU; ChengkuiQu; XiaomingYang

    2005-01-01

    Background/Aims: Hepatocyte growth factor (HGF) regulates proliferation of hepatic stem cells. Transcription factor nuclear factor kappa B (NF-κB) has been demonstrated as a key mediator for cell growth regulation. We investigated the role of NF-κB in HGF-mediated cellular proliferation responses in a rat liver.derived hepatic stem-like cell line WB.F344. Methods: Cell proliferation was determined by incorporation of [3H]thymidine. Phosphorylation of ERK1/2, p38 MAPK, Akt and IκBα by HGF stimulation was detected by Western blotting. NF-κB activation was determined by electrophoretic mobility shift assay and NF-κB.mediated SEAP reporter assay. NF-κB activation was inhibited by treatment with an IκBα dominant-negative vector or inhibitor BAY-11-7082. Results: We found that stimulation of WB-F344 cells with HGF promoted cell proliferation and effectively protected WB-F344 cells from apoptosis induced by TNF-α. We also observed activation of ERK1/2, p38 MAPK, Akt and NF-κB signaling pathways by HGF in WB-F344 cells. HGF-induced cell proliferation was partly blocked by pre-treatment of the cells with inhibitors against MEK1 or p38 MAPK, and completely blocked using an inhibitor for NF-κB activity.Furthermore, it was demonstrated that IκB mutant that suppressed NF-κB activity completely blocked HGF-induced cell proliferation. Conclusions: NF-κB activity is required for HGF-induced proliferation in hepatic stem-like cell line WB-F344, and this activity requires ERK1/2 and p38 MAPK pathways.

  18. Cyclic isoDGR and RGD peptidomimetics containing bifunctional diketopiperazine scaffolds are integrin antagonists.

    Science.gov (United States)

    Panzeri, Silvia; Zanella, Simone; Arosio, Daniela; Vahdati, Leila; Dal Corso, Alberto; Pignataro, Luca; Paolillo, Mayra; Schinelli, Sergio; Belvisi, Laura; Gennari, Cesare; Piarulli, Umberto

    2015-04-13

    The cyclo[DKP-isoDGR] peptidomimetics 2-5, containing bifunctional diketopiperazine (DKP) scaffolds that differ in the configuration of the two DKP stereocenters and in the substitution at the DKP nitrogen atoms, were prepared and examined in vitro in competitive binding assays with purified αv β3 and αv β5 integrin receptors. IC50 values ranged from low nanomolar (ligand 3) to submicromolar with αv β3 integrin. The biological activities of ligands cyclo[DKP3-RGD] 1 and cyclo[DKP3-isoDGR] 3, bearing the same bifunctional DKP scaffold and showing similar αV β3 integrin binding values, were compared in terms of their cellular effects in human U373 glioblastoma cells. Compounds 1 and 3 displayed overlapping inhibitory effects on the FAK/Akt integrin activated transduction pathway and on integrin-mediated cell infiltration processes, and qualify therefore, despite the different RGD and isoDGR sequences, as integrin antagonists. Both compounds induced apoptosis in glioma cells after 72 hour treatment.

  19. Standardization of Image Quality Analysis – ISO 19264

    DEFF Research Database (Denmark)

    Kejser, Ulla Bøgvad; Wüller, Dietmar

    2016-01-01

    There are a variety of image quality analysis tools available for the archiving world, which are based on different test charts and analysis algorithms. ISO has formed a working group in 2012 to harmonize these approaches and create a standard way of analyzing the image quality for archiving...... systems. This has resulted in three documents that have been or are going to be published soon. ISO 19262 defines the terms used in the area of image capture to unify the language. ISO 19263 describes the workflow issues and provides detailed information on how the measurements are done. Last...... but not least ISO 19264 describes the measurements in detail and provides aims and tolerance levels for the different aspects. This paper will present the new ISO 19264 technical specification to analyze image quality based on a single capture of a multi-pattern test chart, and discuss the reasoning behind its...

  20. Neurofibromatosis type 2 tumor suppressor protein, NF2, induces proteasome-mediated degradation of JC virus T-antigen in human glioblastoma.

    Directory of Open Access Journals (Sweden)

    Sarah Beltrami

    Full Text Available Neurofibromatosis type 2 protein (NF2 has been shown to act as tumor suppressor primarily through its functions as a cytoskeletal scaffold. However, NF2 can also be found in the nucleus, where its role is less clear. Previously, our group has identified JC virus (JCV tumor antigen (T-antigen as a nuclear binding partner for NF2 in tumors derived from JCV T-antigen transgenic mice. The association of NF2 with T-antigen in neuronal origin tumors suggests a potential role for NF2 in regulating the expression of the JCV T-antigen. Here, we report that NF2 suppresses T-antigen protein expression in U-87 MG human glioblastoma cells, which subsequently reduces T-antigen-mediated regulation of the JCV promoter. When T-antigen mRNA was quantified, it was determined that increasing expression of NF2 correlated with an accumulation of T-antigen mRNA; however, a decrease in T-antigen at the protein level was observed. NF2 was found to promote degradation of ubiquitin bound T-antigen protein via a proteasome dependent pathway concomitant with the accumulation of the JCV early mRNA encoding T-antigen. The interaction between T-antigen and NF2 maps to the FERM domain of NF2, which has been shown previously to be responsible for its tumor suppressor activity. Co-immunoprecipitation assays revealed a ternary complex among NF2, T-antigen, and the tumor suppressor protein, p53 within a glioblastoma cell line. Further, these proteins were detected in various degrees in patient tumor tissue, suggesting that these associations may occur in vivo. Collectively, these results demonstrate that NF2 negatively regulates JCV T-antigen expression by proteasome-mediated degradation, and suggest a novel role for NF2 as a suppressor of JCV T-antigen-induced cell cycle regulation.

  1. ISO26000系列讲座:ISO26000制定过程

    Institute of Scientific and Technical Information of China (English)

    陈元桥

    2011-01-01

    @@ 1创新独特的工作机制 ISO/TMB(国际标准化组织技术管理局)希望以ISO 26000的制定为契机,进行全面的开拓性探索,开展国际标准化创新试验:一方面是标准内容创新,即:国际标准化的内容将不再仅限于传统的技术领域,而是扩展到包括社会科学领域在内的所有领域,正如时任ISO/TMB副主席的ZivaPatir女士所言,"只要市场需要且符合ISO战略,没有什么是国际标准化工作不可以涉及的","我们的传统任务是促进产品、服务、过程、材料和系统的标准化.

  2. Usability evaluation of mobile applications using ISO 9241 and ISO 25062 standards.

    Science.gov (United States)

    Moumane, Karima; Idri, Ali; Abran, Alain

    2016-01-01

    This paper presents an empirical study based on a set of measures to evaluate the usability of mobile applications running on different mobile operating systems, including Android, iOS and Symbian. The aim is to evaluate empirically a framework that we have developed on the use of the Software Quality Standard ISO 9126 in mobile environments, especially the usability characteristic. To do that, 32 users had participated in the experiment and we have used ISO 25062 and ISO 9241 standards for objective measures by working with two widely used mobile applications: Google Apps and Google Maps. The QUIS 7.0 questionnaire have been used to collect measures assessing the users' level of satisfaction when using these two mobile applications. By analyzing the results we highlighted a set of mobile usability issues that are related to the hardware as well as to the software and that need to be taken into account by designers and developers in order to improve the usability of mobile applications.

  3. Paraptosis and NF-κB activation are associated with protopanaxadiol-induced cancer chemoprevention

    Directory of Open Access Journals (Sweden)

    Wang Chong-Zhi

    2013-01-01

    Full Text Available Abstract Background Protopanaxadiol (PPD is a triterpenoid that can be prepared from steamed ginseng. PPD possesses anticancer potential via caspase-dependent apoptosis. Whether paraptosis, a type of the caspase-independent cell death, is also induced by PPD has not been evaluated. Methods Cell death, the cell cycle and intracellular reactive oxygen species (ROS were analyzed by flow cytometry after staining with annexin V/PI, PI/RNase or H2DCFDA. We observed morphological changes by crystal violet staining assay. Mitochondrial swelling was measured by ultraviolet–visible spectrophotometry. The activation of NF-κB was measured by luciferase reporter assay. Results At comparable concentrations of 5-fluorouracil, PPD induced more cell death in human colorectal cancer cell lines HCT-116 and SW-480. We demonstrated that PPD induced paraptosis in these cancer cells. PPD treatment significantly increased the percentage of cancer cells with cytoplasmic vacuoles. After the cells were treated with PPD and cycloheximides, cytoplasmic vacuole generation was inhibited. The paraptotic induction effect of PPD was also supported by the results of the mitochondrial swelling assay. PPD induced ROS production in cancer cells, which activated the NF-κB pathway. Blockage of ROS by NAC or PS-1145 inhibited the activation of NF-κB signaling. Conclusions PPD induces colorectal cancer cell death in part by induction of paraptosis. The anticancer activity of PPD may be enhanced by antioxidants such as green tea, which also inhibit the activation of NF-κB signaling.

  4. Sertifikatõ ISO - mafioznaja struktura so znakom pljuss / Olesja Bõkova

    Index Scriptorium Estoniae

    Bõkova, Olesja

    2005-01-01

    Esimesed ISO sertifikaadid, mille aluseks on briti kvaliteedi standardite süsteem, anti Euroopa Liidus välja 1987. a. ISO sertifikaadid Eestis. Diagramm: Välja antud ISO 9000 sertifikaadid 1994-2004

  5. Identification of large NF1 duplications reciprocal to NAHR-mediated type-1 NF1 deletions.

    Science.gov (United States)

    Kehrer-Sawatzki, Hildegard; Bengesser, Kathrin; Callens, Tom; Mikhail, Fady; Fu, Chuanhua; Hillmer, Morten; Walker, Martha E; Saal, Howard M; Lacassie, Yves; Cooper, David N; Messiaen, Ludwine

    2014-12-01

    Approximately 5% of all patients with neurofibromatosis type-1 (NF1) exhibit large deletions of the NF1 gene region. To date, only nine unrelated cases of large NF1 duplications have been reported, with none of the affected patients exhibiting multiple café au lait spots (CALS), Lisch nodules, freckling, or neurofibromas, the hallmark signs of NF1. Here, we have characterized two novel NF1 duplications, one sporadic and one familial. Both index patients with NF1 duplications exhibited learning disabilities and atypical CALS. Additionally, patient R609021 had Lisch nodules, whereas patient R653070 exhibited two inguinal freckles. The mother and sister of patient R609021 also harbored the NF1 duplication and exhibited cognitive dysfunction but no CALS. The breakpoints of the nine NF1 duplications reported previously have not been identified and hence their underlying generative mechanisms have remained unclear. In this study, we performed high-resolution breakpoint analysis that indicated that the two duplications studied were mediated by nonallelic homologous recombination (NAHR) and that the duplication breakpoints were located within the NAHR hotspot paralogous recombination site 2 (PRS2), which also harbors the type-1 NF1 deletion breakpoints. Hence, our study indicates for the first time that NF1 duplications are reciprocal to type-1 NF1 deletions and originate from the same NAHR events.

  6. Effects of returning NF concentrate on the MBR-NF process treating antibiotic production wastewater.

    Science.gov (United States)

    Li, Kun; Cheng, Yutao; Wang, Jianxing; Zhang, Junya; Liu, Jibao; Yu, Dawei; Li, Mingyue; Wei, Yuansong

    2016-07-01

    The optimization of the nanofiltration (NF) concentrate backflow ratio (R cb) and the influence of the NF concentrate on the performance of membrane bioreactor-nanofiltration (MBR-NF) process treating antibiotic production wastewater were investigated on a laboratory scale. The R cb was optimized at 60 % based on the removal rates of chemical oxygen demand (COD) and NH4 (+)-N by MBR. Data analyses indicated that salinity brought by NF concentrate is the major driver leading to the decrease of sludge activity, especially at a high R cb. EPS analysis showed that electric conductivity (EC), proteins in soluble microbial products (SMP), and SMP brought by NF concentrate are the dominant factors causing the severe membrane fouling in MBR. Furthermore, undegradable substances including fulvic acid-like and humic acid-like compounds accumulated in NF concentrate showed significant influence on fouling of NF. MBR could well degrade small MW compounds in NF concentrate, which confirmed the enhancement of organic removal efficiency by recycling the NF concentrate to MBR. The MBR-NF process showed a relatively stable performance at the R cb of 60 % (volume reduction factor (VRF) = 5), and the NF permeate could satisfy the water quality standard for fermentation process with a water recovery rate of 90.9 %.

  7. The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri.

    Science.gov (United States)

    Sohn, Hae-Jin; Kim, Jong-Hyun; Shin, Myeong-Heon; Song, Kyoung-Ju; Shin, Ho-Joon

    2010-03-01

    Naegleria fowleri destroys target cells by trogocytosis, a phagocytosis mechanism, and a process of piecemeal ingestion of target cells by food-cups. Phagocytosis is an actin-dependent process that involves polymerization of monomeric G-actin into filamentous F-actin. However, despite the numerous studies concerning phagocytosis, its role in the N. fowleri food-cup formation related with trogocytosis has been poorly reported. In this study, we cloned and characterized an Nf-actin gene to elucidate the role of Nf-actin gene in N. fowleri pathogenesis. The Nf-actin gene is composed of 1,128-bp and produced a 54.1-kDa recombinant protein (Nf-actin). The sequence identity was 82% with nonpathogenic Naegleria gruberi but has no sequence identity with other mammals or human actin gene. Anti-Nf-actin polyclonal antibody was produced in BALB/c mice immunized with recombinant Nf-actin. The Nf-actin was localized on the cytoplasm, pseudopodia, and especially, food-cup structure (amoebastome) in N. fowleri trophozoites using immunofluorescence assay. When N. fowleri co-cultured with Chinese hamster ovary cells, Nf-actin was observed to localize around on phagocytic food-cups. We also observed that N. fowleri treated with cytochalasin D as actin polymerization inhibitor or transfected with antisense oligomer of Nf-actin gene had shown the reduced ability of food-cup formation and in vitro cytotoxicity. Finally, it suggests that Nf-actin plays an important role in phagocytic activity of pathogenic N. fowleri.

  8. ANKRD1 modulates inflammatory responses in C2C12 myoblasts through feedback inhibition of NF-κB signaling activity

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xin-Hua [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Bauman, William A. [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Cardozo, Christopher, E-mail: chris.cardozo@va.gov [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States)

    2015-08-14

    Transcription factors of the nuclear factor-kappa B (NF-κB) family play a pivotal role in inflammation, immunity and cell survival responses. Recent studies revealed that NF-κB also regulates the processes of muscle atrophy. NF-κB activity is regulated by various factors, including ankyrin repeat domain 2 (AnkrD2), which belongs to the muscle ankyrin repeat protein family. Another member of this family, AnkrD1 is also a transcriptional effector. The expression levels of AnkrD1 are highly upregulated in denervated skeletal muscle, suggesting an involvement of AnkrD1 in NF-κB mediated cellular responses to paralysis. However, the molecular mechanism underlying the interactive role of AnkrD1 in NF-κB mediated cellular responses is not well understood. In the current study, we examined the effect of AnkrD1 on NF-κB activity and determined the interactions between AnkrD1 expression and NF-κB signaling induced by TNFα in differentiating C2C12 myoblasts. TNFα upregulated AnkrD1 mRNA and protein levels. AnkrD1-siRNA significantly increased TNFα-induced transcriptional activation of NF-κB, whereas overexpression of AnkrD1 inhibited TNFα-induced NF-κB activity. Co-immunoprecipitation studies demonstrated that AnkrD1 was able to bind p50 subunit of NF-κB and vice versa. Finally, CHIP assays revealed that AnkrD1 bound chromatin at a NF-κB binding site in the AnrkD2 promoter and required NF-κB to do so. These results provide evidence of signaling integration between AnkrD1 and NF-κB pathways, and suggest a novel anti-inflammatory role of AnkrD1 through feedback inhibition of NF-κB transcriptional activity by which AnkrD1 modulates the balance between physiological and pathological inflammatory responses in skeletal muscle. - Highlights: • AnkrD1 is upregulated by TNFα and represses NF-κB-induced transcriptional activity. • AnkrD1 binds to p50 subunit of NF-κB and is recruited to NF-κB bound to chromatin. • AnkrD1 mediates a feed-back inhibitory loop

  9. NF-κB in immunobiology

    Institute of Scientific and Technical Information of China (English)

    Matthew S Hayden; Sankar Ghosh

    2011-01-01

    NF-κB was first discovered and characterized 25 years ago as a key regulator of inducible gene expression in the immune system. Thus, it is not surprising that the clearest biological role of NF-κB is in the development and function of the immune system. Both innate and adaptive immune responses as well as the development and maintenance of the cells and tissues that comprise the immune system are, at multiple steps, under the control of the NF-κB family of transcription factors. Although this is a well-studied area of NF-KB research, new and significant findings continue to accumulate. This review will focus on these areas of recent progress while also providing a broad overview of the roles of NF-κB in mammalian immunobiology.

  10. Three Quality Journeys - Capability Maturity Model Integration, Baldrige Performance Excellence Program, and ISO 9000 Series

    Science.gov (United States)

    2012-04-26

    ISO 9001 :2008 has been implemented by over a million organizations in 176 countries. 14 Three Quality Journeys...University I S O 9 0 0 0 Initial Release of ISO 9000:1987 9001 :2000 amended to 9001 :2008 – quality management requirements 9004:2000 amended...to 9004:2009 to support management of sustained success of an organization Released - ISO 9000:2000 ISO 9001 :2000 ISO 9004:2000 Released - ISO

  11. The stability of iso-α-acids and reduced iso-α-acids in stored blood specimens.

    Science.gov (United States)

    Rodda, Luke N; Gerostamoulos, Dimitri; Drummer, Olaf H

    2014-06-01

    The long-term stability of the iso-α-acids, and three structurally similar but chemically altered iso-α-acids (known as 'reduced iso-α-acids' and consisting of the rho-, tetrahydro- and hexahydro-iso-α-acid groups) were investigated in whole blood. Pools of blank blood spiked with the four beer-specific ingredient congener groups at two different concentration levels were stored at 20°C, 4°C and -20°C; and extracted in duplicate in weeks 1, 3, 5 and 8, using a previously published method. A loss of 15% of the initial concentration was considered to indicate possible instability and losses greater than 30% demonstrated significant losses. The individual analytes within the four iso-α-acid groups were also measured to determine which iso-α-acids were subject to greater degradation and were responsible for the overall group instability. All four iso-α-acid groups showed significant losses after 8 weeks of storage under room temperature conditions in particularly the natural iso-α-acid group where major losses were observed (96% and 85% losses for low and high concentrations, respectively). Some degradation in all iso-α-acid groups were seen at 4°C samples predominantly due to the 'n' analogs of the groups showing an increased instability in blood. The -20°C storage conditions resulted in minimal changes in concentrations of all analytes. Higher than frozen storage temperatures can result in substantial changes on the stability of the iso-α-acid type groups in blood. The aim of this study was to highlight the stabilities of the IAA analytes in order to assist in the interpretation of IAA in stored blood specimens.

  12. ISO Spectroscopy of Proto-Planetary Nebulae

    Science.gov (United States)

    Hrivnak, Bruce J.

    2000-01-01

    The goal of this program was to determine the chemical properties of the dust shells around protoplanetary nebulae (PPNs) through a study of their short-wavelength (6-45 micron) infrared spectra. PPNs are evolved stars in transition from the asymptotic giant branch to the planetary nebula stages. Spectral features in the 10 to 20 gm region indicate the chemical nature (oxygen- or carbon-rich), and the strengths of the features relate to the physical properties of the shells. A few bright carbon-rich PPNs have been observed to show PAH features and an unidentified 21 micron emission feature. We used the Infrared Space Observatory (ISO) to observe a sample of IRAS sources that have the expected properties of PPNs and for which we have accurate positions. Some of these have optical counterparts (proposal SWSPPN01) and some do not (SWSPPN02). We had previously observed these from the ground with near-infrared photometry and, for those with visible counterparts, visible photometry and spectroscopy, which we have combined with these new ISO data in the interpretation of the spectra. We have completed a study of the unidentified emission feature at 21 micron in eight sources. We find the shape of the feature to be the same in all of the sources, with no evidence of any substructure. The ratio of the emission peak to continuum ranges from 0.13 to 1.30. We have completed a study of seven PPNs and two other carbon-rich objects for which we had obtained ISO 2-45 micron observations. The unidentified emission features at 21 and 30 micron were detected in six sources, including four new detections of the 30 micron feature. This previously unresolved 30 micron feature was resolved and found to consist of a broad feature peaking at 27.2 micron (the "30 micron" feature) and a narrower feature peaking at 25.5 micron (the "26 micron" feature). This new 26 micron feature is detected in eight sources and is particularly strong in IRAS Z02229+6208 and 16594-4656. The unidentified

  13. UBE2W抑制TNFα诱导的NF-κB转录活性%The Inhibition of TNFα Induced NF-κB Transcription Activity by UBE2W

    Institute of Scientific and Technical Information of China (English)

    王少鑫; 巩伟丽; 陈媛; 靳宝锋

    2012-01-01

    UBE2 W is a novel ubiquitin-conjugating enzyme ( E2 ) , and its function has not been well defined. Dual-luciferase reporter assay system ( pNF-κB-luc) and Western blot were used to evaluate the effects of UBE2W on the TNFα-induced NF-kB transcription activity when UBE2W was knocked down with siRNA or overex-pressed. The result indicates that UBE2W can significantly inhibit TNFa-induced NF-kB transcription activity and suggestes that UBE2W might participate in the regulation of NF-κB-associated functions.%UBE2W (ubiquitin-conjugating enzyme E2W)是一新的泛素结合酶,关于其功能研究甚少.采用双荧光报告系统和Western blot等方法,分别干涉和过表达UBE2W,分析UBE2W对TNF(刺激下NF-(B转录活性的影响.结果显示UBE2W能够显著抑制TNFα诱导的NF-κB的转录活性,提示该基因可能参与NF-κB相关功能的调控.

  14. ADOPTION OF ISO 9001 QUALITY MANAGEMENT STANDARD IN AFRICA

    Directory of Open Access Journals (Sweden)

    Erlantz Allur

    2014-03-01

    Full Text Available This article analyzes the dissemination of ISO 9001, the main global management standards, within Africa. The work refers to the diffusion of ISO 9001 standard in terms of its certification intensity. In this article, the dissemination of ISO 9001 in Africa has been analyzed. The findings reveal that the diffusion of the standard in this continent is not very relevant, what might be seen as an indicator of the process of Africa's trade marginalization in the age of globalization. The general certification intensity of the continet is of 0.18; in other words, the proportion of the contribution of Africa to the global GDP of the world is more than five times superior to the proportion of ISO 9001 certificates located in Africa. By means of the logistic model the dissemination of ISO 9001 is forecasted, and it has been observed that the diffusion of ISO 9001 in Africa is in an 85% of its saturation point. Taking into account this model, it's expected that the dissemination of ISO 9001 will be growing until 2020.

  15. Complexing properties of {alpha}-iso-saccharinate: thorium

    Energy Technology Data Exchange (ETDEWEB)

    Allard, S.; Ekberg, C. [Nuclear Chemistry - Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Goeteborg (Sweden)

    2005-07-01

    Full text of publication follows: In the alkaline conditions of the intended Swedish repository for long-lived, low to intermediate level waste, SFL3-5, cellulose based items will degrade with iso-saccharinate being the dominant end-product. Iso-saccharinate is a strong complexing agent for tetravalent actinides, as well as for trivalent lanthanides, and its complexation properties are of interest for the long-term repository assessment. In the presented study, the complexation of {sup 234}Th-{alpha}-iso-saccharinate is examined using liquid-liquid- extraction and radioanalytical techniques. An organic phase consisting of 0.1 M acetylacetone in toluene was used with a 1.0 M NaClO{sub 4} aqueous phase kept at pH 8 in a thermostated AKUFVE unit. Since the {alpha}-iso-saccharinic system consists of three species - the carboxylic {alpha}-iso-saccharinic acid, {alpha}-iso-saccharinate and {alpha}-D-iso-saccharino-1,4-lactone - care must be taken when choosing the operational pH. Keeping the aqueous phase at pH 8 results in negligible concentrations of all but the {alpha}-iso-saccharinate form while at the same time being an excellent pH for the {sup 234}Th-acetylacetone complexation with very limited hydrolysis effect. The {alpha}- iso-saccharinate concentration was incrementally increased, and samples of equal volumes were taken from the two phases and analyzed in a liquid scintillation detector once the radioactive equilibrium between {sup 234}Th and {sup 234}Pa had been reached. Two complexation constants were used to fit a distribution equation to the experimental data. (authors)

  16. Protonation and lactonization of {alpha}-iso-saccharinic acid

    Energy Technology Data Exchange (ETDEWEB)

    Rao, L.; Tian, G. [Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Zhang, Z. [Washington State University, Pullman, WA 99165 (United States); Rai, D. [Pacific Northwest National Laboratory, Richland, WA 99352 (United States)

    2005-07-01

    Full text of publication follows: {alpha}-iso-saccharinic acid, a degradation product of cellulose materials, has been a subject of study due to its ability to complex actinides and affect their migration behavior in the environment. Like other poly-hydroxy-carboxylic acids such as gluconic acid, {alpha}-iso-saccharinic acid undergoes dehydration to form {alpha}-iso-saccharinate-1,4-lactone in acidic solutions. The lactonization of iso-saccharinic acid is slower than the protonation/deprotonation reactions because it involves breaking and formation of C-O as well as O-H bonds. It is important to take into consideration the slow lactonization reaction when determining the protonation constant of iso-saccharinic acid. In this work, the protonation of a-iso-saccharinate and the lactonization of {alpha}-iso-saccharinic acid have been evaluated by potentiometry. A concept of 'composite' protonation constant, similar to the well-known composite protonation constant of carbonic acid [1], is developed for {alpha}- iso-saccharinic acid. Results on the protonation and lactonization of {alpha}-iso-saccharinic acid are discussed in comparison with gluconic acid. This research was supported by the Office of Science, Office of Basic Energy Sciences and Office of Biological and Environmental Research, U.S. Department of Energy, under Contract No. DE-AC03-76SF0098 at Lawrence Berkeley National Laboratory. [1] Stumm, W., Morgan, J.J.: 'Aquatic Chemistry: an Introduction Emphasizing Chemical Equilibria in Natural Waters', John Wiley and Sons, New York (1981), pp. 173-174. (authors)

  17. Relationship between Carbachol Hyperstimulation-induced Pancreatic Intracelluar Trypsinogen and NF-кB Activation in Rats in vitro

    Institute of Scientific and Technical Information of China (English)

    Chunfang JIANG; Hai ZHENG; Sunan LIU; Kaifeng FANG

    2008-01-01

    The relationship between intracelluar trypsinogen activation and NF-r,B activation in rat pancreatic acinar cells induced by M3 cholinergic receptor agonist (carbachoi) hyperstimulation was studied. Rat pancreatic acinar cells were isolated, cultured and treated with carbachol, the active pro- tease inhibitor (pefabloc) and NF-кB inhibitor (PDTC) in vitro. Intracelluar trypsin activity was measured by using a fluorogenie substrate. The activity of NF-кB was monitored by using electro- phoretic mobility shift assay. The results showed that after pretreatment with 2 mmol/L pefabloc, the activities of trypsin and NF-кB in pancreatic acinar cells treated with high concertrations of carbachol (10-3 mol/L) in vitro was significantly decreased as compared with control group (P<0.01). The addi- tion of 10-2mol/L PDTC resulted in a significant decrease of NF-кB activities in pancreatic acinar cells after treated with high concertrations of carbachol (10-3 mol/L) in vitro, but the intracelluar trypsinogen activity was not obviously inhibited (P>0.05). It was concluded that intracelluar trypsi- nogen activation is likely involved in the regulation of high concertrations of carbachol-induced NF-кB activation in pancreatic acinar cells in vitro. NF-кB activation is likely not necessary for high concertrations of carbachol-induced trypsinogen activation in pancreatic acinar cells in vitro.

  18. HSV-1-induced activation of NF-κB protects U937 monocytic cells against both virus replication and apoptosis.

    Science.gov (United States)

    Marino-Merlo, Francesca; Papaianni, Emanuela; Medici, Maria Antonietta; Macchi, Beatrice; Grelli, Sandro; Mosca, Claudia; Borner, Christoph; Mastino, Antonio

    2016-09-01

    The transcription factor nuclear factor-kappa B (NF-κB) is a crucial player of the antiviral innate response. Intriguingly, however, NF-κB activation is assumed to favour herpes simplex virus (HSV) infection rather than restrict it. Apoptosis, a form of innate response to viruses, is completely inhibited by HSV in fully permissive cells, but not in cells incapable to fully sustain HSV replication, such as immunocompetent cells. To resolve the intricate interplay among NF-κB signalling, apoptosis and permissiveness to HSV-1 in monocytic cells, we utilized U937 monocytic cells in which NF-κB activation was inhibited by expressing a dominant-negative IκBα. Surprisingly, viral production was increased in monocytic cells in which NF-κB was inhibited. Moreover, inhibition of NF-κB led to increased apoptosis following HSV-1 infection, associated with lysosomal membrane permeabilization. High expression of late viral proteins and induction of apoptosis occurred in distinct cells. Transcriptional analysis of known innate response genes by real-time quantitative reverse transcription-PCR excluded a contribution of the assayed genes to the observed phenomena. Thus, in monocytic cells NF-κB activation simultaneously serves as an innate process to restrict viral replication as well as a mechanism to limit the damage of an excessive apoptotic response to HSV-1 infection. This finding may clarify mechanisms controlling HSV-1 infection in monocytic cells.

  19. Suppressing progress of pancreatitis through selective inhibition of NF-κB activation by using NAC

    Institute of Scientific and Technical Information of China (English)

    赵志成; 郑树森; 陈文亮; 王选; 齐莹

    2004-01-01

    Objective:To explore the characteristics of NF-κB activation in the progress of pancreatitis,the relationship with expression of TNF-α in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC.Method:Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A).NAC served as the inhibitor of NF-κB activation.In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-κB activation was detected with flow cytometry (FCM) and the expression of TNF-α mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. Result:NF-κB activation in the pancreatitis group was higher than that in the control group (P<0.01),peaked at 3 hours,and was depressed by the inhibitor of NF-κB, NAC.The expression of TNF-α as well as the level of lipase and amylase in the serum also rose synchronously with activation of NF-κB.In contrast to group A, it was significantly different (P<0.01) in group B.After using NAC in group C,all of these values were decreased and the inflammatory reaction in the pancreas abated evidently. The pathology changes of the pancreas were shown to be alleviated in group C. Conclusion: First,NF-κB activity is intensively initiated in the course of pancreatitis and shown to have closely relationship with the release of cytokines. Second, use of NAC markedly depressed NF-κB activation. TNF-α expression is down regulated by cytokines. It is suggested that NAC probably acts as a useful agent for treatment of pancreatitis by indirectly inhibiting activation of NF-κB.

  20. The historical impact of ISO 9000 on Lebanese firms.

    Science.gov (United States)

    Mezher, Toufic; Ajam, Maher; Shehab, Mohamad

    2004-01-01

    The objective of this paper is to assess the impact of the new ISO 9001:2000 standard on the Quality Management Systems (QMS) of Lebanese firms that were already certified under ISO 9000:1994. To get an accurate feedback of stakeholders in a firm, three different questionnaires were developed and distributed to management, employees, and customers respectively. Empirical results indicate that ISO 9001:2000 has improved the QMS performance of Lebanese firms over that of the 1994 edition. Nevertheless, results showed that the new standard still has some weaknesses when it comes to improving suppliers' relationships and empowering employees.

  1. Experience of 2 dental clinics registered to ISO 9002.

    Science.gov (United States)

    Casas, Michael J; Kenny, David J; Johnston, Douglas H

    2003-04-01

    This paper describes the 3-year experience of managing 2 hospital-based dental clinics registered to ISO 9002:1994; it also examines the revision of previous quality management standards in 2 separate institutions to prepare for registration under the new ISO 9001:2000 standard. Daily equipment and process checks, combined with internal audits, were the backbone of the quality system at both locations. Corrective and protective actions had been underused, because of the partial duplication produced by 2 different institutionally mandated risk management and incident reporting systems. ISO 9002 registration provided both dental clinics with responsive quality systems, emphasizing patient satisfaction and providing measurable continuous quality improvement.

  2. Modeling and Implementing ISO 13584-based Part Library

    Institute of Scientific and Technical Information of China (English)

    杨东; 肖丽雯; 何援军; 张申生

    2004-01-01

    ISO 13584 (I.e. PLIB) is an international standard for the representation and exchange of CAD part libraries. It aims to provide an application-independent mechanism to enable the share of part library information between applications. In this paper, the approach of modeling part library conforming to ISO 13584 is presented. Also, a prototype of part library management system, I.e. BYL-PLIB, whose implementation is in agreement with ISO 13854 is developed to demonstrate the usefulness of proposed approach.

  3. ISO 26000 – An Integrative Approach of Corporate Social Responsibility

    Directory of Open Access Journals (Sweden)

    Herciu Mihaela

    2016-04-01

    Full Text Available ISO 26000 represents a guidance on corporate social responsibility and it is, at the present time, one of the most important document on CSR in the world. Without being mandatory or regulated, ISO 26000 is focus on seven core subjects that have to be approached synergistic – governance, human right, labor, environment, business practices, consumer, and community – in order to achieve its goal. The present paper intends to present and analyze the seven core subjects of ISO 26000 from tow perspectives: by emphasizing the benefits of implementing the standard into the business strategy and by revealing the correlation between corporate social responsibility and sustainable development.

  4. The ISO SWS on-line system

    Science.gov (United States)

    Roelfsema, P. R.; Kester, D. J. M.; Wesselius, P. R.; Wieprech, E.; Sym, N.

    1992-01-01

    The software which is currently being developed for the Short Wavelength Spectrometer (SWS) of the Infrared Space Observatory (ISO) is described. The spectrometer has a wide range of capabilities in the 2-45 micron infrared band. SWS contains two independent gratings, one for the long and one for the short wavelength section of the band. With the gratings a spectral resolution of approximately 1000 to approximately 2500 can be obtained. The instrument also contains two Fabry-Perault's yielding a resolution between approximately 1000 and approximately 20000. Software is currently being developed for the acquisition, calibration, and analysis of SWS data. The software is firstly required to run in a pipeline mode without human interaction, to process data as they are received from the telescope. However, both for testing and calibration of the instrument as well as for evaluation of the planned operating procedures the software should also be suitable for interactive use. Thirdly the same software will be used for long term characterization of the instrument. The software must work properly within the environment designed by the European Space Agency (ESA) for the spacecraft operations. As a result strict constraints are put on I/O devices, throughput etc.

  5. "ENVIRONMENTAL AUDITING STANDARDS ISO14010, 14011, 14012 "

    Directory of Open Access Journals (Sweden)

    J.Nouri

    1998-09-01

    Full Text Available In order to standardize sustainable status of environmental management in productive and industrial activities and commercial and economical relationship, the ISO 14000 standards series guarantee the minimization of the costs, growth accoptancy of society and trade and environmental quality improvement and natural resources. In fact, these standards will help any organization to improve and solve these standards and help to improve and solve their environmental remarks of executive activities in the best way. Environmental auditing is one of the environmental tools. In the early 1970, most organization was searching ways to match their regulations with environmental needs. Although in some case, environmental auditing had been compulsory by the legal authorities, directors, were doing environmental auditing by their own ways, in order to escape from liabi1ities and punishments. At a large scale, efficiency and proportion of environmental management system are evaluated through a periodical management review, by the acquired environmental programmed progress. Thus, environmental system performance output connects and evaluates to the input of organization management review process. In the meantime, one of the important inputs to management review process in environmental auditing.

  6. BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

    Institute of Scientific and Technical Information of China (English)

    Li-Hui ZHAO; Xue-Qing BA; Xiao-Guang WANG; Xiao-Juan ZHU; Li WANG; Xian-Lu ZENG

    2005-01-01

    Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg 1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP Ⅱ in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated, but not the resting,T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP Ⅱ, and this interaction is closely dependent on the activation of gene transcription.

  7. Introduction to the New Edition of ISO/DIS 9004%新版ISO/DIS 9004介绍

    Institute of Scientific and Technical Information of China (English)

    谷艳君

    2009-01-01

    @@ 一、概述 ISO自发布第一个ISO 9000族标准以来,至今已有20多年.在这么长的时间里,作为ISO 9000族核心标准之一的ISO 9004,在世界范围内一直都没能得到广泛应用.究其原因,一方面存在ISO 9000族标准的使用者在认知和功利上的因素,另一方面也不排除ISO 9004标准的目的、思路和内容不太适合使用者的因素.因此,TC 176正在对ISO 9004做重大修订.目前,修订工作已进入到DIS阶段,计划2009年8月正式发布新版ISO 9004标准.

  8. AGRO100 inhibits activation of nuclear factor-kappaB (NF-kappaB) by forming a complex with NF-kappaB essential modulator (NEMO) and nucleolin.

    Science.gov (United States)

    Girvan, Allicia C; Teng, Yun; Casson, Lavona K; Thomas, Shelia D; Jüliger, Simone; Ball, Mark W; Klein, Jon B; Pierce, William M; Barve, Shirish S; Bates, Paula J

    2006-07-01

    AGRO100, also known as AS1411, is an experimental anticancer drug that recently entered human clinical trials. It is a member of a novel class of antiproliferative agents known as G-rich oligonucleotides (GRO), which are non-antisense, guanosine-rich phosphodiester oligodeoxynucleotides that form stable G-quadruplex structures. The biological activity of GROs results from their binding to specific cellular proteins as aptamers. One important target protein of GROs has been previously identified as nucleolin, a multifunctional protein expressed at high levels by cancer cells. Here, we report that AGRO100 also associates with nuclear factor-kappaB (NF-kappaB) essential modulator (NEMO), which is a regulatory subunit of the inhibitor of kappaB (IkappaB) kinase (IKK) complex, and also called IKKgamma. In the classic NF-kappaB pathway, the IKK complex is required for phosphorylation of IkappaBalpha and subsequent activation of the transcription factor NF-kappaB. We found that treatment of cancer cells with AGRO100 inhibits IKK activity and reduces phosphorylation of IkappaBalpha in response to tumor necrosis factor-alpha stimulation. Using a reporter gene assay, we showed that AGRO100 blocks both tumor necrosis factor-alpha-induced and constitutive NF-kappaB activity in human cancer cell lines derived from cervical, prostate, breast, and lung carcinomas. In addition, we showed that, in AGRO100-treated cancer cells, NEMO is coprecipitated by nucleolin, indicating that both proteins are present in the same complex. Our studies suggest that abrogation of NF-kappaB activity may contribute to the anticancer effects of AGRO100 and that nucleolin may play a previously unknown role in regulating the NF-kappaB pathway.

  9. Prevalence of bortezomib-resistant constitutive NF-kappaB activity in mantle cell lymphoma

    Directory of Open Access Journals (Sweden)

    Kahl Brad S

    2008-05-01

    Full Text Available Abstract Background The proteasome inhibitor bortezomib can inhibit activation of the transcription factor NF-κB, a mechanism implicated in its anti-neoplastic effects observed in mantle cell lymphoma (MCL. However, NF-κB can be activated through many distinct mechanisms, including proteasome independent pathways. While MCL cells have been shown to harbor constitutive NF-κB activity, what fraction of this activity in primary MCL samples is sensitive or resistant to inhibition by bortezomib remains unclear. Results Proteasome activity in the EBV-negative MCL cell lines Jeko-1 and Rec-1 is inhibited by greater than 80% after exposure to 20 nM bortezomib for 4 hours. This treatment decreased NF-κB activity in Jeko-1 cells, but failed to do so in Rec-1 cells when assessed by electrophoretic mobility shift assay (EMSA. Concurrently, Rec-1 cells were more resistant to the cytotoxic effects of bortezomib than Jeko-1 cells. Consistent with a proteasome inhibitor resistant pathway of activation described in mouse B-lymphoma cells (WEHI231 and a breast carcinoma cell line (MDA-MB-468, the bortezomib-resistant NF-κB activity in Rec-1 cells is inhibited by calcium chelators, calmodulin inhibitors, and perillyl alcohol, a monoterpene capable of blocking L-type calcium channels. Importantly, the combination of perillyl alcohol and bortezomib is synergistic in eliciting Rec-1 cell cytotoxicity. The relevance of these results is illuminated by the additional finding that a considerable fraction of primary MCL samples (8 out of 10 displayed bortezomib-resistant constitutive NF-κB activity. Conclusion Our findings show that bortezomib-resistant NF-κB activity is frequently observed in MCL samples and suggest that this activity may be relevant to MCL biology as well as serve as a potential therapeutic target.

  10. NF-kappaB Is Involved in the Regulation of EMT Genes in Breast Cancer Cells

    Science.gov (United States)

    Mencalha, Andre L.; Ferreira, Gerson M.; de Souza, Waldemir F.; Morgado-Díaz, José A.; Maia, Amanda M.; Corrêa, Stephany; Abdelhay, Eliana S. F. W.

    2017-01-01

    The metastatic process in breast cancer is related to the expression of the epithelial-to-mesenchymal transition transcription factors (EMT-TFs) SNAIL, SLUG, SIP1 and TWIST1. EMT-TFs and nuclear factor-κB (NF-κB) activation have been associated with aggressiveness and metastatic potential in carcinomas. Here, we sought to examine the role of NF-κB in the aggressive properties and regulation of EMT-TFs in human breast cancer cells. Blocking NF-κB/p65 activity by reducing its transcript and protein levels (through siRNA-strategy and dehydroxymethylepoxyquinomicin [DHMEQ] treatment) in the aggressive MDA-MB-231 and HCC-1954 cell lines resulted in decreased invasiveness and migration, a downregulation of SLUG, SIP1, TWIST1, MMP11 and N-cadherin transcripts and an upregulation of E-cadherin transcripts. No significant changes were observed in the less aggressive cell line MCF-7. Bioinformatics tools identified several NF-κB binding sites along the promoters of SNAIL, SLUG, SIP1 and TWIST1 genes. Through chromatin immunoprecipitation and luciferase reporter assays, the NF-κB/p65 binding on TWIST1, SLUG and SIP1 promoter regions was confirmed. Thus, we suggest that NF-κB directly regulates the transcription of EMT-TF genes in breast cancer. Our findings may contribute to a greater understanding of the metastatic process of this neoplasia and highlight NF-κB as a potential target for breast cancer treatment. PMID:28107418

  11. Blood-based NfL

    Science.gov (United States)

    Janelidze, Shorena; Hall, Sara; Magdalinou, Nadia; Lees, Andrew J.; Andreasson, Ulf; Norgren, Niklas; Linder, Jan; Forsgren, Lars; Constantinescu, Radu; Zetterberg, Henrik; Blennow, Kaj

    2017-01-01

    Objective: To determine if blood neurofilament light chain (NfL) protein can discriminate between Parkinson disease (PD) and atypical parkinsonian disorders (APD) with equally high diagnostic accuracy as CSF NfL, and can therefore improve the diagnostic workup of parkinsonian disorders. Methods: The study included 3 independent prospective cohorts: the Lund (n = 278) and London (n = 117) cohorts, comprising healthy controls and patients with PD, progressive supranuclear palsy (PSP), corticobasal syndrome (CBS), and multiple system atrophy (MSA), as well as an early disease cohort (n = 109) of patients with PD, PSP, MSA, or CBS with disease duration ≤3 years. Blood NfL concentration was measured using an ultrasensitive single molecule array (Simoa) method, and the diagnostic accuracy to distinguish PD from APD was investigated. Results: We found strong correlations between blood and CSF concentrations of NfL (ρ ≥ 0.73–0.84, p ≤ 0.001). Blood NfL was increased in patients with MSA, PSP, and CBS (i.e., all APD groups) when compared to patients with PD as well as healthy controls in all cohorts (p < 0.001). Furthermore, in the Lund cohort, blood NfL could accurately distinguish PD from APD (area under the curve [AUC] 0.91) with similar results in both the London cohort (AUC 0.85) and the early disease cohort (AUC 0.81). Conclusions: Quantification of blood NfL concentration can be used to distinguish PD from APD. Blood-based NfL might consequently be included in the diagnostic workup of patients with parkinsonian symptoms in both primary care and specialized clinics. Classification of evidence: This study provides Class III evidence that blood NfL levels discriminate between PD and APD. PMID:28179466

  12. Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time

    KAUST Repository

    Hwang, Yoon-Hyung

    2016-01-11

    Plant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S::OsNF-YC2 nf-yc3 nf-yc4 nf-yc9 plants was attributed to the upregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). In vitro and in planta protein–protein analyses revealed that OsNF-YB8/10/11 and OsNF-YC1/2/4/6/7 interact with AtNF-YC3/4/9 and AtNF-YB2/3, respectively. Our data indicate that some OsNF-YB and OsNF-YC genes are functional equivalents of AtNF-YB2/3 and AtNF-YC3/4/9 genes, respectively, and suggest functional conservation of Arabidopsis and rice NF-Y genes in the control of flowering time.

  13. ISO 9000标准与ISO/TS 16949技术规范

    Institute of Scientific and Technical Information of China (English)

    陶泳; 朱文玉

    2005-01-01

    根据ISO的报道,当前标准的变化有两个趋势,一是各类专业标准日益增多,有专业的延伸,有范围上的扩展.另一个趋势是标准的集成化或整合,即相关标准集成一个统一体.本文论述了TS 16949与ISO 9000标准的关系及贯彻TS 16949的必要性.

  14. Prolongation of liver allograft survival by dendritic cells modified with NF-κB decoy oligodeoxynucleotides

    Institute of Scientific and Technical Information of China (English)

    Ming-Qing Xu; Yu-Ping Suo; Jian-Ping Gong; Ming-Man Zhang; Lü-Nan Yan

    2004-01-01

    AIM: To induce the tolerance of rat liver allograft by dendritic cells (DCs) modified with NF-κB decoy oligodeoxynucleotides (ODNs).METHODS: Bone marrow (BM)-derived DCs from SD rats were propagated in the presence of GM-CSF or GM-CSF+IL-4to obtain immature DCs or mature DCs. GM-CSF+IL-4-propagated DCs were treated with double-strand NF-κB decoy ODNs containing two NF-κB binding sites or scrambled ODNs to ascertain whether NF-κB decoy ODNs might prevent DC maturation. GM-CSF-propagated DCs, GMCSF+NF-κB decoy ODNs or scrambled ODNs-propagated DCs were treated with LPS for 18 h to determine whether NF-κB decoy ODNs could prevent LPS-induced IL-12production in DCs. NF-κB binding activities, costimulatory molecule (CD40, CD80, CD86) surface expression, IL-12protein expression and allostimulatory capacity of DCs were measured with electrophoretic mobility shift assay (EMSA),flow cytometry, Western blotting, and mixed lymphocyte reaction (MLR), respectively. GM-CSF-propagated DCs, GMCSF+IL-4 -propagated DCs, and GM-CSF+NF-κB decoy ODNs or scrambled ODNs-propagated DCs were injected intravenously into recipient LEW rats 7 d prior to liver transplantation and immediately after liver transplantation.Histological grading of liver graft rejection was determined 7 d after liver transplantation. Expression of IL-2, IL-4 and IFN-γ mRNA in liver graft and in recipient spleen was analyzed by semiquantitative RT-PCR. Apoptosis of liver allograft-infiltrating cells was measured with TUNEL staining.RESULTS: GM-CSF-propagated DCs, GM-CSF+NF-κB decoy ODNs-propagated DCs and GM-CSF+ scrambled ODNspropagated DCs exhibited features of immature DCs, with similar low level of costimulatory molecule(CD40, CD80,CD86) surface expression, absence of NF-κB activation,and few allocostimulatory activities. GM-CSF+IL-4-propagated DCs displayed features of mature DCs, with high levels of costimulatory molecule (CD40, CD80, CD86) surface expression, marked NF-κB activation, and

  15. Technical drawings Extract from international standard ISO 1101

    CERN Document Server

    1983-01-01

    The document forms an extract of ISO 1101, suitable for everyday use. Symbols and tolerancing characteristics as well as examples of indication and interpretation are listed in a table which is given in English and French.

  16. Applicability of ISO 16697 Data to Spacecraft Fire Fighting Strategies

    Science.gov (United States)

    Hirsch, David B.; Beeson, Harold D.

    2012-01-01

    Presentation Agenda: (1) Selected variables affecting oxygen consumption during spacecraft fires, (2) General overview of ISO 16697, (3) Estimated amounts of material consumed during combustion in typical ISS enclosures, (4) Discussion on potential applications.

  17. ISO 9000质量体系与ISO 14000环境管理体系结合研究%A synthetical research to the ISO 9000 quality system and ISO14000 environment management system

    Institute of Scientific and Technical Information of China (English)

    于启武; 徐泓; 易验

    1999-01-01

    本研究报告分为两部分:第一部分,体系结合研究,包括体系结合问题的由来、ISO 9000质量体系与ISO 14000环境管理体系的推动因素比较、对国家有关政策的建议、企业实施ISO 9000和ISO 14000建立质量和环境综合管理体系的方式和程序;第二部分,环境成本研究,包括环境成本现状分析、环境成本核算的必要性和理论基础、环境成本的基本内容和分类、环境成本的核算方法.

  18. ISO and EIGA standards for cryogenic vessels and accessories

    CERN Document Server

    CERN. Geneva

    2016-01-01

    The EIGA/WG 6’s scope is cryogenic vessels and accessories, including their design, material compatibility, operational requirements and periodical inspection. The specific responsibilities include monitoring international standardization (ISO, CEN) and regulations (UN, TPED, PED...

  19. A review of ISO and CEN standards on ergonomics

    NARCIS (Netherlands)

    Dul, J.; Vlaming, P.M. de; Munnik, M.J.

    1996-01-01

    In recent years an increasing number of official ergonomic standards has become available world-wide. The International Standardization Organization (ISO) and the European standardization organization Comite Europeen de Normalisation (CEN) have published 28 ergonomic standards, whereas 69 standards

  20. The new ISO 9001:2015 : Its opportunities and challenges

    OpenAIRE

    Sickinger-Nagorni, Rajka; Schwanke, Jana

    2016-01-01

    The aim of this thesis is to introduce business supportive quality management systems as a whole and to further scrutinize main additions and changes of the new quality management system standard ISO 9001:2015, regarding benefits or adverse effects for implementation. The revised standard was published in autumn 2015 by ISO (International Organisation for Standardization) and already a few companies worldwide have been certified to it. Although there are organisations which have utilized the ...

  1. Discussion on the Relationship of ISO26000 and School Environmental Management%ISO26000与学校环境管理关系浅析

    Institute of Scientific and Technical Information of China (English)

    陈晓燕

    2015-01-01

    This article introduces the connotation of ISO26000 and school environmental management and describes the rela-tionship between ISO26000 and school environmental management.It puts forward the opinion on managing the school environ-ment with the ISO 26000.%本文介绍了ISO26000社会责任国际标准和学校环境管理的内涵,阐述了ISO26000与学校环境管理的关系,提出了学校引入ISO26000,进行学校环境管理的观点。

  2. A novel intestinal trans-factor (NF-LPH1) interacts with the lactase-phlorizin hydrolase promoter and co-varies with the enzymatic activity

    DEFF Research Database (Denmark)

    Troelsen, J T; Norén, O; Sjöström, H

    1992-01-01

    The promoter of the pig lactase-phlorizin hydrolase was cloned and showed to be functional in the human intestinal cell line Caco2. The proximal promoter was analyzed for binding of nuclear proteins from small intestine and liver. DNase I footprinting and electrophoretic mobility shift assays show......, that an intestinal nuclear factor (NF-LPH1) binds to a sequence (-40 to -54) located close to the TATA-box. Enterocytes from newborn pigs with high lactase activity contain high amounts of NF-LPH1, whereas enterocytes from adult pigs with low lactase activity contain low amounts of NF-LPH1. The liver does...... not contain lactase activity, and NF-LPH1 is not present in liver nuclear extracts in detectable amounts. This indicates that NF-LPH1 is involved in the decline of lactase at weaning and may be of importance for the molecular explanation of hypolactasia in humans. It was demonstrated by transfection of two...

  3. Iso-vector and Iso-scalar Tensor Charges of the Nucleon from Lattice QCD

    CERN Document Server

    Bhattacharya, Tanmoy; Cohen, Saul; Gupta, Rajan; Joseph, Anosh; Lin, Huey-Wen; Yoon, Boram

    2015-01-01

    We present results for the iso-vector and flavor diagonal tensor charges $g^{u-d}_T$, $g^{u}_T$, $g^{d}_T$, and $g^{s}_T$ needed to probe novel tensor interactions at the TeV scale in neutron and nuclear $\\beta$-decays and the contribution of the quark electric dipole moment (EDM) to the neutron EDM. The lattice QCD calculations were done using nine ensembles of gauge configurations generated by the MILC collaboration using the HISQ action with 2+1+1 dynamical flavors. These ensembles span three lattice spacings $a \\approx 0.06, 0.09$ and $0.12 $ fm and three quark masses corresponding to the pion masses $M_\\pi \\approx 130, 220$ and $310 $ MeV. Using estimates from these ensembles, we quantify all systematic uncertainties and perform a simultaneous extrapolation in the lattice spacing, volume and light quark masses for the connected contributions. The final estimates of the connected nucleon (proton) tensor charge for the iso-vector combination is $g_T^{u-d} = 1.020(76) $ in the $\\overline{\\text{MS}}$ scheme ...

  4. Surviving the Transition from FGDC to ISO Metadata Standards

    Science.gov (United States)

    Fox, C. G.; Milan, A.; Sylvester, D.; Habermann, T.; Kozimor, J.; Froehlich, D.

    2008-12-01

    The NOAA Metadata Manager and Repository (NMMR) has served a well established group of data managers at NOAA's National Data Centers for over a decade. It provides a web interface for managing FGDC compliant metadata and publishing that metadata to several large data discovery systems (GeoSpatial One-Stop, NASA's Global Change Master Directory, the Comprehensive Large-Array data Stewardship System, and FirstGov). The Data Center's are now faced with migration of these metadata to new International Metadata Standards (ISO 19115, 19115-2, "). We would like to accomplish this migration while minimizing disruption to the current users and supporting significant new capabilities of the ISO standards. Our current approach involves relational ISO views on top of the existing XML database to convert FGDC content into ISO without changing the data manager interface. These views are the foundation for ISO- compliant XML metadata access via REST-like web services. Additionally, new database tables provide information required by ISO that is not included in the FGDC standard. This approach allows us to support the new standard without disrupting the current system.

  5. Inter ISO Market Coordination by Calculating Border Locational Marginal Prices

    Directory of Open Access Journals (Sweden)

    BABIC, A. B.

    2013-05-01

    Full Text Available In this paper the methodology for solving Locational Marginal Price (LMP differences (inconsistency of LMPs that arise at the boundary buses between separate power markets is proposed. The algorithm developed enables us to obtain consistent LMP values at the boundary buses between interconnected ISOs. A Primal-Dual Interior Point based optimal power flow (OPF is applied, with complete set of power system physical limit constraints, to solve a regional spot market. The OPF is implemented such that producer and consumer behaviors are modeled simultaneously, while the welfare is maximized. In this paper a generalized methodology for multiple ISOs case is proposed and later it is practically applied on two interconnected independent entities. The algorithm for approximation of cost coefficients of generators and dispatchable loads for neighboring ISOs is proposed. The developed algorithm enables participating ISOs to obtain LMPs at the boundary buses with other interconnected ISOs. By controlling interchange of electric power at the scheduled level, regional spot markets are resolved eliminating possible exercise of market power by individual interconnected ISOs. Results of proposed methodology are tested on the IEEE 118-bus power system.

  6. Somatic neurofibromatosis type 1 (NF1) inactivation characterizes NF1-associated pilocytic astrocytoma.

    Science.gov (United States)

    Gutmann, David H; McLellan, Michael D; Hussain, Ibrahim; Wallis, John W; Fulton, Lucinda L; Fulton, Robert S; Magrini, Vincent; Demeter, Ryan; Wylie, Todd; Kandoth, Cyriac; Leonard, Jeffrey R; Guha, Abhijit; Miller, Christopher A; Ding, Li; Mardis, Elaine R

    2013-03-01

    Low-grade brain tumors (pilocytic astrocytomas) arising in the neurofibromatosis type 1 (NF1) inherited cancer predisposition syndrome are hypothesized to result from a combination of germline and acquired somatic NF1 tumor suppressor gene mutations. However, genetically engineered mice (GEM) in which mono-allelic germline Nf1 gene loss is coupled with bi-allelic somatic (glial progenitor cell) Nf1 gene inactivation develop brain tumors that do not fully recapitulate the neuropathological features of the human condition. These observations raise the intriguing possibility that, while loss of neurofibromin function is necessary for NF1-associated low-grade astrocytoma development, additional genetic changes may be required for full penetrance of the human brain tumor phenotype. To identify these potential cooperating genetic mutations, we performed whole-genome sequencing (WGS) analysis of three NF1-associated pilocytic astrocytoma (PA) tumors. We found that the mechanism of somatic NF1 loss was different in each tumor (frameshift mutation, loss of heterozygosity, and methylation). In addition, tumor purity analysis revealed that these tumors had a high proportion of stromal cells, such that only 50%-60% of cells in the tumor mass exhibited somatic NF1 loss. Importantly, we identified no additional recurrent pathogenic somatic mutations, supporting a model in which neuroglial progenitor cell NF1 loss is likely sufficient for PA formation in cooperation with a proper stromal environment.

  7. IAF发布从ISO 14001:1996到ISO 14001:2004转换计划

    Institute of Scientific and Technical Information of China (English)

    CNAB技术管理处

    2005-01-01

    目前,国际标准化组织(ISO)正在就ISO/FDIS 14001:2004征求意见。FDIS即国际标准最终草案稿。ISO计划2004年年底前发布该标准,即ISO 14001:2004,同时发布对此次换版的政策建议书。为此,国际认可论坛(IAF)针对从ISO 14001:1996到ISO 14001:2004的转换制定了相应的计划,具体内容为:

  8. Molecular Regulation of Endothelial Cells by NF-1

    Science.gov (United States)

    2013-01-01

    33 1 Introduction: NF1 and Vascular Disease Mutations in the NF1 gene cause Neurofibromatosis type 1, an autosomal dominant...to increase in the population(McClatchey 2007). NF1 has variable clinical manifestations. Most commonly changes in skin pigmentation (café au-lait...Importantly, a significant clinical manifestation of NF1 disease can include vascular disease. Patients with NF1 disease make up a significant portion

  9. The NF1 gene revisited – from bench to bedside

    OpenAIRE

    Yap, Yoon-Sim; McPherson, John R.; Ong, Choon-Kiat; Rozen, Steven G.; Teh, Bin-Tean; Lee, Ann SG; Callen, David F.

    2014-01-01

    Neurofibromatosis type 1 (NF1) is a relatively common tumour predisposition syndrome related to germline aberrations of NF1, a tumour suppressor gene. The gene product neurofibromin is a negative regulator of the Ras cellular proliferation pathway, and also exerts tumour suppression via other mechanisms. Recent next-generation sequencing projects have revealed somatic NF1 aberrations in various sporadic tumours. NF1 plays a critical role in a wide range of tumours. NF1 alterations appear to b...

  10. Steroid Hormones in NF1 Tumorigenesis

    Science.gov (United States)

    2005-08-01

    neurofibroma and MPNST Schwann cells. We found less than 2-fold difference in these transcripts in tumor versus normal Schwann cells (in those that changed...neurofibromin-negative) to steroid hormones, focusing on estrogen and progesterone. The hypothesis is that human neurofibroma (and MPNST , malignant...to determine steroid hormone receptor expression in human normal, NF1 neurofibroma, and NF1 MPNST Schwann cells, pre- and post-hormone treatment by

  11. 77 FR 11532 - Notice of Attendance at ISO New England and NEPOOL Meetings

    Science.gov (United States)

    2012-02-27

    ... Federal Energy Regulatory Commission Notice of Attendance at ISO New England and NEPOOL Meetings The... Commission staff may attend upcoming ISO New England Inc. (ISO-NE) and New England Power Pool (NEPOOL... Commission staff may monitor the various meetings posted on the ISO-NE Web site. NEPOOL...

  12. Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla

    Directory of Open Access Journals (Sweden)

    Junjun Li

    2014-01-01

    Full Text Available Background Information. NF-κB signaling pathway plays a complicated role in the biological functions of mesenchymal stem cells. However, the effects of NF-κB pathway on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs remain unclear. The present study was designed to evaluate the effects of canonical NF-κB pathway on the osteo/odontogenic capacity of SCAPs in vitro. Results. Western blot results demonstrated that NF-κB pathway in SCAPs was successfully activated by TNF-α or blocked by BMS-345541. NF-κB pathway-activated SCAPs presented a higher proliferation activity compared with control groups, as indicated by dimethyl-thiazol-diphenyl tetrazolium bromide assay (MTT and flow cytometry assay (FCM. Wound scratch assay revealed that NF-κB pathway-activated SCAPs presented an improved migration capacity, enhanced alkaline phosphatase (ALP activity, and upregulated mineralization capacity of SCAPs, as compared with control groups. Meanwhile, the odonto/osteogenic markers (ALP/ALP, RUNX2/RUNX2, OSX/OSX, OCN/OCN, OPN/OPN, BSP/BSP, DSPP/DSP, and DMP-1/DMP-1 in NF-κB pathway-activated SCAPs were also significantly upregulated as compared with control groups at both protein and mRNA levels. However, NF-κB pathway-inhibited SCAPs exhibited a lower proliferation/migration capacity, and decreased odonto/osteogenic ability in comparison with control groups. Conclusion. Our findings suggest that classical NF-κB pathway plays a paramount role in the proliferation and committed differentiation of SCAPs.

  13. Foxtail millet NF-Y families: genome-wide survey and evolution analyses identified two functional genes important in abiotic stresses

    Directory of Open Access Journals (Sweden)

    Zhi-Juan eFeng

    2015-12-01

    Full Text Available It was reported that Nuclear Factor Y (NF-Y genes were involved in abiotic stress in plants. Foxtail millet (Setaria italica, an elite stress tolerant crop, provided an impetus for the investigation of the NF-Y families in abiotic responses. In the present study, a total of 39 NF-Y genes were identified in foxtail millet. Synteny analyses suggested that foxtail millet NF-Y genes had experienced rapid expansion and strong purifying selection during the process of plant evolution. De novo transcriptome assembly of foxtail millet revealed 11 drought up-regulated NF-Y genes. SiNF-YA1 and SiNF-YB8 were highly activated in leaves and/or roots by drought and salt stresses. Abscisic acid (ABA and H2O2 played positive roles in the induction of SiNF-YA1 and SiNF-YB8 under stress treatments. Transient luciferase (LUC expression assays revealed that SiNF-YA1 and SiNF-YB8 could activate the LUC gene driven by the tobacco (Nicotiana tobacam NtERD10, NtLEA5, NtCAT, NtSOD or NtPOD promoter under normal or stress conditions. Overexpression of SiNF-YA1 enhanced drought and salt tolerance by activating stress-related genes NtERD10 and NtCAT1 and by maintaining relatively stable relative water content (RWC and contents of chlorophyll, superoxide dismutase (SOD, peroxidase (POD, catalase (CAT and malondialdehyde (MDA in transgenic lines under stresses. SiNF-YB8 regulated expression of NtSOD, NtPOD, NtLEA5 and NtERD10 and conferred relatively high RWC and chlorophyll contents and low MDA content, resulting in drought and osmotic tolerance in transgenic lines under stresses. Therefore, SiNF-YA1 and SiNF-YB8 could activate stress-related genes and improve physiological traits, resulting in tolerance to abiotic stresses in plants. All these results will facilitate functional characterization of foxtail millet NF-Ys in future studies.

  14. Foxtail Millet NF-Y Families: Genome-Wide Survey and Evolution Analyses Identified Two Functional Genes Important in Abiotic Stresses.

    Science.gov (United States)

    Feng, Zhi-Juan; He, Guan-Hua; Zheng, Wei-Jun; Lu, Pan-Pan; Chen, Ming; Gong, Ya-Ming; Ma, You-Zhi; Xu, Zhao-Shi

    2015-01-01

    It was reported that Nuclear Factor Y (NF-Y) genes were involved in abiotic stress in plants. Foxtail millet (Setaria italica), an elite stress tolerant crop, provided an impetus for the investigation of the NF-Y families in abiotic responses. In the present study, a total of 39 NF-Y genes were identified in foxtail millet. Synteny analyses suggested that foxtail millet NF-Y genes had experienced rapid expansion and strong purifying selection during the process of plant evolution. De novo transcriptome assembly of foxtail millet revealed 11 drought up-regulated NF-Y genes. SiNF-YA1 and SiNF-YB8 were highly activated in leaves and/or roots by drought and salt stresses. Abscisic acid (ABA) and H2O2 played positive roles in the induction of SiNF-YA1 and SiNF-YB8 under stress treatments. Transient luciferase (LUC) expression assays revealed that SiNF-YA1 and SiNF-YB8 could activate the LUC gene driven by the tobacco (Nicotiana tobacam) NtERD10, NtLEA5, NtCAT, NtSOD, or NtPOD promoter under normal or stress conditions. Overexpression of SiNF-YA1 enhanced drought and salt tolerance by activating stress-related genes NtERD10 and NtCAT1 and by maintaining relatively stable relative water content (RWC) and contents of chlorophyll, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA) in transgenic lines under stresses. SiNF-YB8 regulated expression of NtSOD, NtPOD, NtLEA5, and NtERD10 and conferred relatively high RWC and chlorophyll contents and low MDA content, resulting in drought and osmotic tolerance in transgenic lines under stresses. Therefore, SiNF-YA1 and SiNF-YB8 could activate stress-related genes and improve physiological traits, resulting in tolerance to abiotic stresses in plants. All these results will facilitate functional characterization of foxtail millet NF-Ys in future studies.

  15. ISO 19011:2002标准的思考

    Institute of Scientific and Technical Information of China (English)

    陈乃骏

    2003-01-01

    @@ISO 19011:2002《质量和(或)环境管理体系审核 指南》(下称新版审核标准)已于2002年10月发布.该标准取代了三个质量审核标准 (ISO 10011-1、ISO 10011-2、ISO10011-3)和三个环境管理体系审核标准(ISO1401O、ISO 14011、ISO 14012).新版审核标准……

  16. Cadenas de rodillos. Tendencias de desarrollo y dimensionales según normas ISO. // Trends of roller chain and dimensional series according to ISO standards.

    Directory of Open Access Journals (Sweden)

    M. E. García Domínguez

    2007-05-01

    Full Text Available El artículo es una compilación de resultados derivados de un estudio del desarrollo de la transmisión por cadena de rodillosque permite brindar información relevante sobre la tendencia del mercado internacional, innovaciones recientes en lascadenas de rodillos y las diferencias dimensionales que caracterizan las series geométricas de las cadenas ISO-A e ISO-Bsegún la norma ISO 606:2004.Palabras claves: Cadena de rodillos, ISO 606, dimensiones de cadenas de rodillos.______________________________________________________________________________Abstract:The paper is a compilation of some results associated with the development of roller chain transmissions and outstanding informationin relations with the roller chain market trend, recent innovations and dimensional differences of ISO-A and ISO-B roller chain seriesaccording to ISO Standard 606:2004.Key Words: roller chain, ISO 606, roller chain dimension.

  17. ISO 9000∶2000--新的国际质量标准

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    从1987年以来,全世界已有25万个机构通过了ISO 9000鉴定。新标准ISO 9000∶2000要求被鉴定机构更新它们老的ISO 9000程序。ISO 9000∶2000比ISO 9000∶1994前进了一大步。

  18. Livscyklusvurderinger - en kommenteret oversættelse af ISO 14040 til 14043

    DEFF Research Database (Denmark)

    Jerland, J.; Christiansen, K.; Weidema, B.

    . Standarderne er DS/EN ISO 14040 Livscyklusvurdering. Principper og opbygning DS/EN ISO 14041 Livscyklusvurdering. Formål og afgrænsning DS/EN ISO 14042 Livscyklusvurdering. Vurdering af miljøpåvirkninger DS/EN ISO 14043 Livscyklusvurdering. Fortolkning af resultater Målet med publikationen er at give brugerne...... af livscyklusvurderinger et samlet værktøj som udgangspunkt for arbejdet med LCA efter ISO-standarderne i 14040 serien....

  19. Recognition of the iso-ADP-ribose moiety in poly(ADP-ribose) by WWE domains suggests a general mechanism for poly(ADP-ribosyl)ation-dependent ubiquitination.

    Science.gov (United States)

    Wang, Zhizhi; Michaud, Gregory A; Cheng, Zhihong; Zhang, Yue; Hinds, Thomas R; Fan, Erkang; Cong, Feng; Xu, Wenqing

    2012-02-01

    Protein poly(ADP-ribosyl)ation and ubiquitination are two key post-translational modifications regulating many biological processes. Through crystallographic and biochemical analysis, we show that the RNF146 WWE domain recognizes poly(ADP-ribose) (PAR) by interacting with iso-ADP-ribose (iso-ADPR), the smallest internal PAR structural unit containing the characteristic ribose-ribose glycosidic bond formed during poly(ADP-ribosyl)ation. The key iso-ADPR-binding residues we identified are highly conserved among WWE domains. Binding assays further demonstrate that PAR binding is a common function for the WWE domain family. Since many WWE domain-containing proteins are known E3 ubiquitin ligases, our results suggest that protein poly(ADP-ribosyl)ation may be a general mechanism to target proteins for ubiquitination.

  20. NF-kappa B activity in T cells stably expressing the Tax protein of human T cell lymphotropic virus type I

    Energy Technology Data Exchange (ETDEWEB)

    Lacoste, J.; Cohen, L.; Hiscott, J. (Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec (Canada))

    1991-10-01

    The effect of constitutive Tax expression on the interaction of NF-{kappa} B with its recognition sequence and on NF-{kappa} B-dependent gene expression was examined in T lymphoid Jurkat cell lines (19D and 9J) stably transformed with a Tax expression vector. Tax expressing T cell lines contained a constitutive level of NF-{kappa} B binding activity, detectable by mobility shift assay and uv cross-linking using a palindromic NF-{kappa} B probe homologous to the interferon beta PRDII site. In Jurkat and NC2.10 induction with phorbol esters resulted in the appearance of new DNA binding proteins of 85, 75, and 54 kDa, whereas in Tax expressing cells the 85-kDa protein and a 92-kDa DNA binding protein were constitutively induced. Expression of Tax protein in 19D and 9J resulted in transcription of the endogenous NF-kappa B-dependent granulocyte-macrophage colony stimulating factor gene and increased basal level expression of transfected NF-kappa B-regulated promoters. Nonetheless transcription of both the endogenous and the transfected gene was inducible by PMA treatment. Tax expression in Jurkat T cells may alter the stoichiometry of NF-kappa B DNA binding proteins and thus change the expression of NF-kappa B-regulated promoters.

  1. ISO celebrates its prolonged life with a video of Jupiter

    Science.gov (United States)

    1997-07-01

    This is excellent news for astronomers and especially for the multinational teams, with leaders in France, Germany, the Netherlands and the United Kingdom, who spent many years devising the four instruments served by ISO's telescope. The camera ISOCAM, the photometer ISOPHOT, the Short Wavelength Spectrometer and the Long Wavelength Spectrometer span between them an unprecedented range of infrared wavelengths from 2 to 200 microns. The atmosphere of Jupiter is one of the cool and cloudy places attracting ISO's attention, and ESA today releases a video of unprecedented images of Jupiter. The planet changes its appearance drastically as the camera ISOCAM scans a range of 90 different infrared wavelengths. Picture by picture, ISOCAM picks out different features of the atmosphere's composition and behaviour. These and other results from ISO will enable scientists to sharpen their ideas about how Jupiter's weather works. "ISO is giving us a new impression of the giant planets of the Solar System," comments Roger Bonnet, ESA's director of science. "Not just Jupiter, but Saturn, Uranus and Neptune too. By observing the planets across its very wide range of infrared wavelengths, ISO can see features overlooked even by spacecraft visiting the planets. The remarkable movie of Jupiter released today represents only a few per cent of ISO's wavelength range, yet every image tells its own story." More information about the Jupiter video appears later in this Information Note. How ISO's cold telescope beat the calendar The need to keep ISO's telescope and instruments chilled to a very low temperature sets a limit to their useful operating life. ISO was supplied with more than 2000 litres of superfluid helium to cool it. Slow evaporation maintains key parts of the spacecraft at temperatures close to absolute zero, below minus 271 degrees C. The rate of loss of helium was expected to be about 3 litres a day, but the cryogenic system could not be tested in exactly the conditions

  2. Iso-suillin from Suillus flavus Induces Apoptosis in Human Small Cell Lung Cancer H446 Cell Line

    Institute of Scientific and Technical Information of China (English)

    Jun-Xia Zhao; Qing-Shuang Zhang; Ying Chen; Sheng-Jie Yao; Yong-Xin Yan; Ying Wang; Jin-Xiu Zhang

    2016-01-01

    Background:The suillin isoform iso-suillin is a natural substance isolated from a petroleum ether extract of the fruiting bodies of the mushroom Suillusflavus.Previous studies have found its inhibition effect on some cancer cells,and we aimed to study its effects on human small cell lung cancer H446 cell line.Methods:Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay.Cellular morphological changes (apoptosis and necrosis) were evaluated using an electron microscope and Hoechst 33258 staining detected by the inverted microscope.Flow cytometry was used to detect cell apoptosis,cell cycle distribution,and mitochondrial membrane potential.Protein expression was determined by Western blotting analysis.Results:Here,we describe the ability of iso-suillin to inhibit the growth of H446 cells in time-and dose-dependent way.Iso-suillin had no obvious impact on normal human lymphocyte proliferation at low concentrations (9.09,18.17,or 36.35 μmol/L) but promoted lymphocyte proliferation at a high concentration (72.70 μmol/L).After treatment of different concentrations of iso-suillin (6.82,13.63,or 20.45 μmol/L),the apoptosis rate of H446 cells increased with increasing concentrations of iso-suillin (16.70%,35.54%,and 49.20%,respectively,all P < 0.05 compared with the control),and the expression of related apoptotic proteins in the mitochondrial pathway including cytochrome c and caspase-9 were up-regulated compared with the control (all P < 0.05).On the contrary,Bcl-2/Bax ratio was down-regulated compared with the control.Besides,the expression of pro-apoptotic proteins in the death receptor apoptosis pathway,including Fas-associating protein with a novel death domain and caspase-8,and the expression of caspase-3,a downstream regulatory protein of apoptosis,were also increased compared with the control (all P < 0.05).Inhibitors of caspase-9 and caspase-8 reversed the apoptosis process in H446 cells to varying

  3. Angiotensin II upregulates endothelial lipase expression via the NF-kappa B and MAPK signaling pathways.

    Directory of Open Access Journals (Sweden)

    Xiaoli Zhang

    Full Text Available BACKGROUND: Angiotensin II (AngII participates in endothelial damage and inflammation, and accelerates atherosclerosis. Endothelial lipase (EL is involved in the metabolism and clearance of high density lipoproteins (HDL, the serum levels of which correlate negatively with the onset of cardiovascular diseases including atherosclerosis. However, the relationship between AngII and EL is not yet fully understood. In this study, we investigated the effects of AngII on the expression of EL and the signaling pathways that mediate its effects in human umbilical vein endothelial cells (HUVECs. METHODS AND FINDINGS: HUVECs were cultured in vitro with different treatments as follows: 1 The control group without any treatment; 2 AngII treatment for 0 h, 4 h, 8 h, 12 h and 24 h; 3 NF-κB activation inhibitor pyrrolidine dithiocarbamate (PDTC pretreatment for 1 h before AngII treatment; and 4 mitogen-activated protein kinase (MAPK p38 inhibitor (SB203580 pretreatment for 1 h before AngII treatment. EL levels in each group were detected by immunocytochemical staining and western blotting. HUVECs proliferation was detected by MTT and proliferating cell nuclear antigen (PCNA immunofluorescence staining. NF-kappa B (NF-κB p65, MAPK p38, c-Jun N-terminal kinase (JNK, extracellular signal-regulated kinase (ERK and phosphorylated extracellular signal-regulated kinase (p-ERK expression levels were assayed by western blotting. The results showed that the protein levels of EL, NF-κB p65, MAPK p38, JNK, and p-ERK protein levels, in addition to the proliferation of HUVECs, were increased by AngII. Both the NF-kB inhibitor (PDTC and the MAPK p38 inhibitor (SB203580 partially inhibited the effects of AngII on EL expression. CONCLUSION: AngII may upregulate EL protein expression via the NF-κB and MAPK signaling pathways.

  4. Implementing ISO standard-compliant freeform component drawings

    Science.gov (United States)

    Youngworth, Richard N.; Kiontke, Sven R.; Aikens, David M.

    2016-07-01

    Successful fabrication of aspheres requires all parts of the process chain including design, production, and measurements. Aspheres now are well-established and accepted as an equal optical element, when done properly. Research and industry have now started to focus efforts to develop the next element that propels the field forward in capability, namely the optical freeform surface. An essential factor enabling wide use of freeforms is communicating requirements. This paper discusses form description and tolerancing additions to ISO 10110 to accommodate freeform surfaces. Information stating how ISO 10110 and related standards documents such as ISO 14999-4 are being continually developed to meet the requirements for specifying freeform surfaces is also provided. This paper further provides an example monolithic freeform element using the recently updated relevant parts of ISO 10110. The first manufacturing of this component has been successful, and this paper shows the role the ISO standard has played in success. Definitions for toleranced parameters, such as surface registration (centration) and form deviation (irregularity, slope, Zernike, PV, and PVr), are also indicated. The monolithic example also shows how to use the defined data and definitions for metrology and data handling. Metrology results for the freeform surface are given.

  5. Pentacyclic Triterpenoids Inhibit IKKβ Mediated Activation of NF-κB Pathway: In Silico and In Vitro Evidences.

    Directory of Open Access Journals (Sweden)

    Kalpesh R Patil

    Full Text Available Pentacyclic Triterpenoids (PTs and their analogues as well as derivatives are emerging as important drug leads for various diseases. They act through a variety of mechanisms and a majority of them inhibit the nuclear factor kappa-beta (NF-κB signaling pathway. In this study, we examined the effects of the naturally occurring PTs on IκB kinase-β (IKKβ, which has great scientific relevance in the NF-κB signaling pathway. On virtual screening, 109 PTs were screened through the PASS (prediction of activity spectra of substances software for prediction of NF-κB inhibitory activity followed by docking on the NEMO/IKKβ association complex (PDB: 3BRV and testing for compliance with the softened Lipinski's Rule of Five using Schrodinger (LLC, New York, USA. Out of the projected 45 druggable PTs, Corosolic Acid (CA, Asiatic Acid (AA and Ursolic Acid (UA were assayed for IKKβ kinase activity in the cell free medium. The UA exhibited a potent IKKβ inhibitory effect on the hotspot kinase assay with IC50 of 69 μM. Whereas, CA at 50 μM concentration markedly reduced the NF-κB luciferase activity and phospho-IKKβ protein expressions. The PTs tested, attenuated the expression of the NF-κB cascade proteins in the LPS-stimulated RAW 264.7 cells, prevented the phosphorylation of the IKKα/β and blocked the activation of the Interferon-gamma (IFN-γ. The results suggest that the IKKβ inhibition is the major mechanism of the PTs-induced NF-κB inhibition. PASS predictions along with in-silico docking against the NEMO/IKKβ can be successfully applied in the selection of the prospective NF-κB inhibitory downregulators of IKKβ phosphorylation.

  6. The role of NF-κB in hepatocellular carcinoma cell

    Institute of Scientific and Technical Information of China (English)

    王剑虹; 黄庆科; 陈民新

    2003-01-01

    Objective To evaluate the role of nuclear factor-kappaB (NF-κB) and IκBα in hepatocellular cacinoma (HCC) SMMC7721 cells, the consequence of NF-κB inhibition in SMMC7721 cells transfected with mutated IκBα (mIκBα) plasmid and the effect of stable inhibition of NF-κB activity in combination with Doxorubicin.Methods Western blot was used to determine the expression of NF-κB and IκBα in SMMC7721 cells and normal liver cells. Nuclear protein was used to evaluate the binding of the 32 P-labeled tandem κB sequence using electrophoretic mobility shift assay and the expression of NF-κB using Western blot between SMMC7721 cells transfected with mIκBα plasmid (SMMC7721-MT) and control cells. Furthermore, cell viability was plotted between SMMC7721-MT and control cells. The binding of κB sequence and cell viability between SMMC7721-MT and control cells at different concentrations of Doxorubicin were also investigated.Results Western blot analysis for nuclear extract showed more P50 (NF-κB1) and P65 (RelA) expression in SMMC7721 cells compared with normal liver cells. The expression of cytosolic IκBα protein in SMMC7721 cells was less than that in normal cells. SMMC7721-MT cells inhibited NF-κB nuclear translocation at 0, 24, 48 and 96 hours. Furthermore, NF-κB cannot be detected in the nuclear protein of SMMC7721-MT cells by Western blot. By calculating cell viability, the proliferation of SMMC7721-MT cells was shown to be suppressed more significantly than that of control cells. NF-κB in untransfected cells was activated by Doxorubicin in a dose-dependent manner, but that in SMMC7721-MT cells was not induced at low concentrations of Doxorubicin. Compared with untransfected cells, the viability of SMMC7721-MT cells was significantly suppressed at the same concentration of Doxorubicin (P<0.01).Conclusions The present study demonstrates that upregulation of NF-κB and downregulation of inhibitory kappaB (IκBα) in SMMC7721 cells are related with the

  7. Multiple roles of NF1 in the melanocyte lineage.

    Science.gov (United States)

    Larribère, Lionel; Utikal, Jochen

    2016-07-01

    NF1 is a tumour suppressor gene, germline mutations of which lead to neurofibromatosis type 1 syndrome. Patients develop benign tumours from several types of cells including neural crest-derived cells. NF1 somatic mutations also occur in 15% of sporadic melanoma, a cancer originating from melanocytes. Evidence now suggests the involvement of NF1 mutations in melanoma resistance to targeted therapies. Although NF1 is ubiquitously expressed, genetic links between NF1 and genes involved in melanocyte biology have been described, implying the lineage-specific mechanisms. In this review, we summarize and discuss the latest advances related to the roles of NF1 in melanocyte biology and in cutaneous melanoma.

  8. Persistent activation of NF-kappaB related to IkappaB's degradation profiles during early chemical hepatocarcinogenesis

    Directory of Open Access Journals (Sweden)

    García-Román Rebeca

    2007-04-01

    Full Text Available Abstract Background To define the NF-kappaB activation in early stages of hepatocarcinogenesis and its IkappaB's degradation profiles in comparison to sole liver regeneration. Methods Western-blot and EMSA analyses were performed for the NF-kappaB activation. The transcriptional activity of NF-kappaB was determined by RT-PCR of the IkappaB-α mRNA. The IkappaB's degradation proteins were determined by Western-blot assay. Results We demonstrated the persistent activation of NF-kappaB during early stages of hepatocarcinogenesis, which reached maximal level 30 min after partial hepatectomy. The DNA binding and transcriptional activity of NF-kappaB, were sustained during early steps of hepatocarcinogenesis in comparison to only partial hepatectomy, which displayed a transitory NF-kappaB activation. In early stages of hepatocarconogenesis, the IkappaB-α degradation turned out to be acute and transitory, but the low levels of IkappaB-β persisted even 15 days after partial hepatectomy. Interestingly, IkappaB-β degradation is not induced after sole partial hepatectomy. Conclusion We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-β and IkappaB-α degradation, mainly IkappaB-β degradation, which contributes to gene transcription related to proliferation required for neoplasic progression.

  9. Maternal Wnt/β-catenin signaling coactivates transcription through NF-κB binding sites during Xenopus axis formation.

    Directory of Open Access Journals (Sweden)

    Neil J Armstrong

    Full Text Available Maternal Wnt/β-Catenin signaling establishes a program of dorsal-specific gene expression required for axial patterning in Xenopus. We previously reported that a subset of dorsally expressed genes depends not only on Wnt/β-Catenin stimulation, but also on a MyD88-dependent Toll-like receptor/IL1-receptor (TLR/IL1-R signaling pathway. Here we show that these two signal transduction cascades converge in the nucleus to coactivate gene transcription in blastulae through a direct interaction between β-Catenin and NF-κB proteins. A transdominant inhibitor of NF-κB, ΔNIκBα, phenocopies loss of MyD88 protein function, implicating Rel/NF-κB proteins as selective activators of dorsal-specific gene expression. Sensitive axis formation assays in the embryo demonstrate that dorsalization by Wnt/β-Catenin requires NF-κB protein activity, and vice versa. Xenopus nodal-related 3 (Xnr3 is one of the genes with dual β-Catenin/NF-κB input, and a proximal NF-κB consensus site contributes to the regional activity of its promoter. We demonstrate in vitro binding of Xenopus β-Catenin to several XRel proteins. This interaction is observed in vivo upon Wnt-stimulation. Finally, we show that a synthetic luciferase reporter gene responds to both endogenous and exogenous β-Catenin levels in an NF-κB motif dependent manner. These results suggest that β-Catenin acts as a transcriptional co-activator of NF-κB-dependent transcription in frog primary embryonic cells.

  10. Effect of Tetrandrine on LPS-induced NF-κB activation in isolated pancreatic acinar cells of rat

    Institute of Scientific and Technical Information of China (English)

    Hong Zhang; Yong-Yu Li; Xian-Zhong Wu

    2006-01-01

    AIM: To investigate the effect of Tetrandrine (Tet) on LPS-induced NF-κB activation and cell injury in pancreatic acinar cells and to explore the mechanism of Tetrandrine preventing LPS-induced acinar cell injury.METHODS: Male rat pancreatic acinar cells were isolated by collagenase digestion, then exposed to LPS (10mg/L), Tet (50 μmol/L, 100 μmol/L) or normal media. At different time point (30 min, 1 h, 4 h, 10 h) after treatment with the agents, cell viability was determined by MTT, the product and nuclear translocation of subunit p65 of NF-κB was visualized by immunofluorescence staining and nuclear protein was extracted to perform EMSA which was used to assay the NF-κB binding activity.RESULTS: LPS induced cell damage directly in a time dependent manner and Tet attenuated LPS-induced cell damage (50 μmol/L, P < 0.05; 100 μmol/L, P < 0.01).NF-κB p65 immunofluorescence staining in cytoplasm increased and began showing its nuclear translocation within 30 min and the peak was shown at 1 h of LPS 10 mg/L treatment. NF-κB DNA binding activity showed the same alteration pattern as p65 immunofluorescence staining. In Tet group, the immunofluorescence staining in cytoplasm and nuclear translocation of NF-κB were inhibited significantly.CONCLUSION: NF-κB activation is an important early event that may contribute to inflammatory responses and cell injury in pancreatic acinar cells. Tet possesses the protective effect on LPS-induced acinar cell injury by inhibiting NF-κB activation.

  11. Mednarodni standardi - veličine in enote (ISO 31-0 do 31-13): International standards - quantities and units (ISO 31-0 to 31-13):

    OpenAIRE

    Glavič, Peter

    2002-01-01

    In this paper the international standards ISO 31 (Quantities and units) are presented with the following parts: ISO 31-0 (General principles), ISO 31-1 (Space and time), ISO 31-2 (Periodic and related phenomena), ISO 31-3 (Mechanics), ISO 31-4 (Heat), ISO 31-5 (Electricity and magnetism), ISO 31-8 (Physical chemistry and molecular physics), ISO 31-12 (Characteristic numbers)and others. The emphasis is given on the basic principles, which is important for writing of reports, presentations, art...

  12. The New Criteria on Quality Certification--ISO 9000:2000 Revised Standards%质量认证的新准则--2000版ISO 9000系列标准

    Institute of Scientific and Technical Information of China (English)

    罗春燕

    2001-01-01

    @@ 国际标准化组织(ISO)于2000年12月15日正式出版了ISO 9000系列质量管理体系国际标准的2000年修订本.即:ISO 9000:2000(取代1994版ISO 8402和ISO 9000-1)、ISO 9001:2000(取代1994版ISO 9001、9002、9003)、ISO9004:2000(取代1994版ISO 9004-1).

  13. A functional genomics approach to (iso)flavonoid glycosylation in the model legume Medicago truncatula.

    Science.gov (United States)

    Modolo, Luzia V; Blount, Jack W; Achnine, Lahoucine; Naoumkina, Marina A; Wang, Xiaoqiang; Dixon, Richard A

    2007-07-01

    Analysis of over 200,000 expressed sequence tags from a range of Medicago truncatula cDNA libraries resulted in the identification of over 150 different family 1 glycosyltransferase (UGT) genes. Of these, 63 were represented by full length clones in an EST library collection. Among these, 19 gave soluble proteins when expressed in E. coli, and these were screened for catalytic activity against a range of flavonoid and isoflavonoid substrates using a high-throughput HPLC assay method. Eight UGTs were identified with activity against isoflavones, flavones, flavonols or anthocyanidins, and several showed high catalytic specificity for more than one class of (iso)flavonoid substrate. All tested UGTs preferred UDP-glucose as sugar donor. Phylogenetic analysis indicated that the Medicago (iso)flavonoid glycosyltransferase gene sequences fell into a number of different clades, and several clustered with UGTs annotated as glycosylating non-flavonoid substrates. Quantitative RT-PCR and DNA microarray analysis revealed unique transcript expression patterns for each of the eight UGTs in Medicago organs and cell suspension cultures, and comparison of these patterns with known phytochemical profiles suggested in vivo functions for several of the enzymes.

  14. Regulation of NF-{kappa}B activity in astrocytes: effects of flavonoids at dietary-relevant concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Spilsbury, Alison [Reading School of Pharmacy, University of Reading, Reading RG6 6UB (United Kingdom); Vauzour, David; Spencer, Jeremy P.E. [Molecular Nutrition Group, Centre for Integrative Neuroscience and Neurodynamics, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP (United Kingdom); Rattray, Marcus, E-mail: m.a.n.rattray@reading.ac.uk [Reading School of Pharmacy, University of Reading, Reading RG6 6UB (United Kingdom)

    2012-02-17

    Highlights: Black-Right-Pointing-Pointer We tested the hypothesis that low concentrations of flavonoids inhibit NF-{kappa}B in astrocytes. Black-Right-Pointing-Pointer Primary cultured astrocytes possess a functional {kappa}B-system, measured using luciferase assays. Black-Right-Pointing-Pointer Seven flavonoids (100 nM-1 {mu}M) failed to reduce NF-{kappa}B activity in astrocytes. Black-Right-Pointing-Pointer Four flavonoids (100 nM-1 {mu}M) failed to reduce TNFa-stimulated NF-{kappa}B activity in astrocytes. Black-Right-Pointing-Pointer (-)-Epicatechin did not regulate nuclear translocation of the NF-{kappa}B subunit, p65. -- Abstract: Neuroinflammation plays an important role in the progression of neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Sustained activation of nuclear transcription factor {kappa}B (NF-{kappa}B) is thought to play an important role in the pathogenesis of neurodegenerative disorders. Flavonoids have been shown to possess antioxidant and anti-inflammatory properties and we investigated whether flavonoids, at submicromolar concentrations relevant to their bioavailability from the diet, were able to modulate NF-{kappa}B signalling in astrocytes. Using luciferase reporter assays, we found that tumour necrosis factor (TNF{alpha}, 150 ng/ml) increased NF-{kappa}B-mediated transcription in primary cultures of mouse cortical astrocytes, which was abolished on co-transfection of a dominant-negative I{kappa}B{alpha} construct. In addition, TNF{alpha} increased nuclear localisation of p65 as shown by immunocytochemistry. To investigate potential flavonoid modulation of NF-{kappa}B activity, astrocytes were treated with flavonoids from different classes; flavan-3-ols ((-)-epicatechin and (+)-catechin), flavones (luteolin and chrysin), a flavonol (kaempferol) or the flavanones (naringenin and hesperetin) at dietary-relevant concentrations (0.1-1 {mu}M) for 18 h. None of the flavonoids modulated constitutive or

  15. ISO 9001 benefits and pitfalls: the path to successful certification.

    Science.gov (United States)

    Krause, M S

    1996-09-01

    The lifeblood of a business is developing and commercializing new products with minimum cost and time and maximum quality. Implementation of a quality management system is often used to achieve these goals, and the ISO 9001 standard for a business quality system is rapidly becoming the model of choice. The existence of an ISO-compliant system is a key to meeting the forthcoming regulatory requirements in the European Union and the US Food and Drug Administrations's proposed good management practices. DuPont has demonstrated leadership in the achievement of ISO registration. I describe the path to these successful registrations along with key lessons from the experience. Elements of success are management commitment, adequate resources, education, communication, total organizational involvement, and auditing of system performance. For the system to flourish and provide benefits to the users, sufficient time must be allocated for the organization to change.

  16. Comparison between ASHRAE and ISO thermal transmittance calculation methods

    DEFF Research Database (Denmark)

    Blanusa, Petar; Goss, William P.; Roth, Hartwig

    2007-01-01

    the Heat Transfer and Total Optical Properties of Fenestration Products, Public Review Draft of Standard 142P, American Society of Heating, Refrigerating and Air Conditioning Engineers, Atlanta, 1998]) and in Europe [ISO 10077-2. Thermal Performance of Windows, Doors and Shutters-Calculation of Thermal...... Transmittance-Part 2: Numerical Method for Frames, International Standards Organization, Geneva, 2003]. The two approaches, called the ASHRAE and ISO methods, are different in the way they treat the effect of the glazing spacer on the heat transfer through the frame and the glazing unit near the frame....... The ASHRAE method assumes that the spacer effects both the heat transfer through the frame and the heat transfer through the glazing in an "edge-of glass" region 63.5mm (2.5in.) from the glazing/frame sight line. The ISO method assumes that the additional heat transfer due to the existence of the spacer...

  17. Considerations about ISO 14001, and suggestions for the next revision

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2006-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001:2004, the international standard for environmental management systems (EMS) with the purpose of improving the next edition in order to recognize and reflect new recognitions in approaches to pollution prevention. The stan......The aim of this paper is to discuss a number of issues related to ISO 14001:2004, the international standard for environmental management systems (EMS) with the purpose of improving the next edition in order to recognize and reflect new recognitions in approaches to pollution prevention....... The standard is a process standard that leaves room for interpretation at company level as well as among lead auditors from certifying bodies. A case study is presented and shows lack of life cycle thinking in product development. The paper suggests changes of ISO 14001:2004 in order to include a clear product...

  18. [History of ISO 15189 and its future perspective].

    Science.gov (United States)

    Kawai, Tadashi

    2010-01-01

    ISO 15189, Medical laboratories--Particular requirements for quality and competence was prepared by ISO/TC212, Clinical laboratory testing and in vitro diagnostic test systems, and its first edition published in 2003 and the second edition in 2007. Its purpose is to be used by medical laboratories in developing their quality management systems and assessing their own competence, also for use by accreditation bodies in confirming or recognizing the competence of medical laboratories. Since its publication, it has been recognized officially by the International Laboratory Accreditation Cooperation (ILAC) to be used as the international standard for the accreditation of medical laboratories worldwide. In Japan, the Japanese Committee for Clinical Laboratory Standards (JCCLS) and the Japan Accreditation Board for Conformity Assessment (JAB) cojointly started the accreditation program in August, 2005, and a total of 46 medical laboratories had been accredited by April 2009. In ISO/TC212/WG1 is working on the preparation of its third edition.

  19. A potential role for NF1 mRNA editing in the pathogenesis of NF1 tumors

    Energy Technology Data Exchange (ETDEWEB)

    Cappione, A.J.; French, B.L.; Skuse, G.R. [Univ. of Rochester School of Medicine and Dentistry, NY (United States)

    1997-02-01

    Neurofibromatosis type I (NF1) is a common disorder that predisposes to neoplasia in tissues derived from the embryonic neural crest. The NF1 gene encodes a tumor suppressor that most likely acts through the interaction of its GTPase-activating protein (GAP)-related domain (GRD) with the product of the ras protooncogene. We have previously identified a site in the NF1 mRNA, within the first half of the NF1 GRD, which undergoes base-modification editing. Editing at that site changes a C to a U, thereby introducing an in-frame stop codon. NF1 RNA editing has been detected in all cell types studied, to date. In order to investigate the role played by editing in NF1 tumorigenesis, we analyzed RNA from 19 NF1 and 4 non-NF1 tumors. We observed varying levels of NF1 mRNA editing in different tumors, with a higher range of editing levels in more malignant tumors (e.g., neurofibrosarcomas) compared to benign tumors (cutaneous neurofibromas). Plexiform neurofibromas have an intermediate range of levels of NF1 mRNA editing. We also compared tumor and nontumor tissues from several NF1 individuals, to determine the extent of variability present in the constitutional levels of NF1 mRNA editing and to determine whether higher levels are present in tumors. The constitutional levels of NF1 mRNA editing varied slightly but were consistent with the levels observed in non-NF1 individuals. In every case, there was a greater level of NF1 mRNA editing in the tumor than in the nontumor tissue from the same patient. These results suggest that inappropriately high levels of NF1 mRNA editing does play a role in NF1 tumorigenesis and that editing may result in the functional equivalent of biallelic inactivation of the NF1 tumor suppressor. 24 refs., 4 figs., 2 tabs.

  20. HERBAL PRODUCT REALIZATION IN ACCORDANCE WITH WHO AND ISO GUIDELINES

    Directory of Open Access Journals (Sweden)

    S. J. Ameh et al

    2012-10-01

    Full Text Available Background: Following the Alma-Ata Declaration of 1978, the World Health Organization (WHO began the issuance of guidelines for developing standardized herbal preparations from Traditional Medicine (TM. Similarly in 1987, the International Organization for Standardization (ISO launched the most anticipated industrial standard (ISO 9001 in world history. The seventh (7th clause of ISO 9001’s eight clauses is devoted to “Product Realization”- a quality management system (QMS procedure that includes: planning of product realization; appreciation of customer-related processes; design and development processes; purchasing; production and service provision; and control of measuring and monitoring equipment. Purpose: The article takes a hard look at the QMS processes involved in product realization and the critical stages of the WHO model of herbal drug development from TM, with a view to devising a framework that can be used to promote the production of quality herbal products, commencing from the stage of ethnobotanical survey, through the laboratory, to the clinic.Methodology: Both the WHO model of herbal drug development and the 7th clause of ISO 9001:2008 were critically reviewed and combined to yield a framework that is discussed within the context of guiding herbal drug development from TM. Results and Discussion: The resulting WHO-ISO framework of herbal product realization is discussed in terms of its relevance to practical problems of GMP-production using herbal starting materials, given their innate variability in composition, potency and appearance. Conclusion: The provisions of ISO 9001’s seventh clause can, to a large extent, be applied to the production of quality herbal products developed in accordance with WHO.

  1. Progress report on the management of the NEA ISOE system

    Energy Technology Data Exchange (ETDEWEB)

    Lazo, E. [OECD Nuclear Energy Agency, Issy-les-Moulineaux (France)

    1995-03-01

    The Information System on Occupational Exposure (ISOE) was launched by the Organization for Economic Cooperation and Development (OECD), Nuclear Energy Agency (NEA) on 1 January, 1992, to facilitate the communication of dosimetric and ALARA implementation data among nuclear utilities around the world. After two years of operation the System has become a mature interactive network for transfer of data and experience. Currently, 37 utilities from 12 countries, representing 289 power plants, and 12 national regulatory authorities participate in ISOE. Agreements for cooperation also exist between the NEA and the Commission of the European Communities (CEC), and the Paris Center of the WOrld Association of Nuclear Operators (WANO-PC). In addition, the International Atomic Energy Agency (IAEA) is acting as a co-sponsor of ISOE for the participation of non-NEA member countries. Three Regional Technical Centres, Europe, Asia, and Non-NEA member countries, serve to administer the system. The ISOE Network is comprised of three data bases and a communications network at several levels. The three ISOE data bases include the following types of information: NEA1 - annual plant dosimetric information; NEA2 - plant operational characteristics for dose and dose rate reduction; and NEA3 - job specific ALARA practices and experiences. The ISOE communications network has matured greatly during 1992 and 1993. In addition to having access to the above mentioned data bases, participants may now solicit information on new subjects, through the Technical Centres, from all other participants on a real-time basis. Information Sheets on these studies are produced for distribution to all participants. In addition, Topical Reports on areas of interest are produced, and Topical Meetings are held annually.

  2. ISO 14000: Review and guidelines for the photovoltaic industry

    Energy Technology Data Exchange (ETDEWEB)

    DePhillips, M.P.

    1997-08-01

    The International Organization for Standardization (ISO) has released and is in the process of ratifying a new set of environmental management system standards, ISO 14000. These voluntary international standards will have a significant impact on how companies throughout the international community manage their environmental programs. Initially this impact will result in increased international trade opportunities due to the anticipated customer requirement for suppliers to demonstrate certification. Other potential impacts will benefit both companies engaged in international commerce and companies with a domestic focus. These would include installing a cohesive system to manage environmental matters, participation within a new regulatory paradigm, and adhering to a potentially new set of legal standards. The wide range of potential environmental, commercial, legal and regulatory benefits offered by ISO 14000 offer most companies significant reason to explore the possibilities of implementing an environmental management system. Organizations will be able to anticipate and meet growing environmental expectations and to ensure ongoing compliance with national and international requirements as well as satisfy the growing demands of customers, stakeholders and the interested public. As an emerging industry with significant international ties, the photovoltaic (PV) community will be effected from these standards if they do become globally adopted. In fact some major players in the PV industry have recently received certification to ISO 9000. This again demonstrates a trend within the industry to move toward a uniform system approach to management. This paper will expose interested parties within the PV industry to the details of ISO 14000 and will assist decision makers assess the various possibilities for implementation certification of ISO 14000 for an individual facility.

  3. Managing your library and its quality the ISO 9001 way

    CERN Document Server

    Balagué, Núria

    2011-01-01

    This book, divided into two parts, provides an introduction to the quality management issues and gives a general overview to the use of ISO 9001 in the library environment. The second part presents the main features of ISO 9001:2008 with practical comments and examples on how to implement its clauses in libraries. Whether in the public or in the private sector, libraries can be seen as service organisations: they act in very dynamic environments where users are increasingly demanding new types of services. Thus the adoption of a quality management system helps each library in meeting the needs

  4. U-value measurements on ISO round Robin window

    DEFF Research Database (Denmark)

    Duer, Karsten

    1997-01-01

    9 laboratories in 6 european and 2 north american countries have participated in a round robin U-value test of a standard facade window. The objective of the round robin test was to verify that the ISO/CEN proposals for U-value measurement standard is robust.......9 laboratories in 6 european and 2 north american countries have participated in a round robin U-value test of a standard facade window. The objective of the round robin test was to verify that the ISO/CEN proposals for U-value measurement standard is robust....

  5. Characterization and robust filtering of multifunctional surfaces using ISO standards

    DEFF Research Database (Denmark)

    Friis, Kasper Storgaard; Godi, Alessandro; De Chiffre, Leonardo

    2011-01-01

    to the multi-process production method involved. A series of MUFU surfaces were characterized by using the ISO 13565 standard for stratified surfaces and it is shown that the standard in some cases is inadequate for the characterization of a MUFU surface. To improve the filtering of MUFU surfaces, the robust...... Gaussian regression filtering technique described in ISO 16610-31 is analyzed and discussed. By slight modifications it is shown how the robust Gaussian regression filter can be applied to remove the form and find a suitable reference surface for further characterization of the MUFU surfaces...

  6. 医疗器械企业如何更好地将ISO 13485与ISO 9001融合应用%How to Better Integrate and Apply ISO 13485 & ISO 9001 Standards for Medical Devices Organizations

    Institute of Scientific and Technical Information of China (English)

    张建锋; 卫志刚

    2016-01-01

    在准确理解标准及其变化内涵的基础上,对比新旧标准的变化,评审原有质量管理体系与ISO 13485:2016与ISO 9001:2015标准的差距,全面策划、更新、完善其质量管理体系使之有效融合,对于医疗器械企业是至关重要的。

  7. Empresas excelentes entre las certificadas en las normas ISO 9000

    OpenAIRE

    Climent Serrano, Salvador

    2005-01-01

    En este trabajo se realiza un estudio entre las empresas certificadas en la norma ISO 9000 de la Comunidad Valenciana mediante una encuesta, con el objetivo de diferenciar los resultados sobre incremento de competitividad por la certificación en las normas ISO 9000, dependiendo de la involucración de las empresas en los sistemas de gestión de la calidad total, así como averiguar que factores inciden para que las empresas certificadas se impliquen en mayor medida en estos sistemas

  8. 2000版ISO 9001标准断想

    Institute of Scientific and Technical Information of China (English)

    李玉峰

    2002-01-01

    @@ 一、质量管理体系文件 ISO 9001:2000标准所要求的是一个"形成文件的质量管理体系",而不是一个"文件体系".也就是说,按ISO 9001:2000标准建立的是质量管理体系,而该质量管理体系的特点之一是"文件化".

  9. STAT3/NF-κB-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer

    Science.gov (United States)

    Kuo, Wei-Ying; Hwu, Luen; Wu, Chun-Yi; Lee, Jhih-Shian; Chang, Chi-Wei; Liu, Ren-Shyan

    2017-01-01

    Triple-negative breast cancer (TNBC) represents approximately 20% of all breast cancers and appears resistance to conventional cytotoxic chemotherapy, demonstrating a particularly poor prognosis and a significantly worse clinical outcome than other types of cancer. Suicide gene therapy has been used for the in vivo treatment of various solid tumors in recent clinical trials. In tumor microenvironment, STAT3/NF-κB pathways are constitutively activated in stromal cells as well as in cancer stem cells (CSCs). In this study, we have cloned a novel STAT3/NF-κB-based reporter system to drive the expression of herpes simplex virus thymidine kinase (HSV-TK) against breast cancer. Lentiviral vector expressing HSV-TK under the regulation of STAT3/NF-κB fused response element was developed. In this setting, we exploited the constitutive STAT3/NF-κB activation in tumors to achieve higher transgene expression than that driven by a constitutively active CMV promotor in vivo. An orthotropic MDA-MB-231 triple negative breast cancer mouse model was used for evaluating the feasibility of STAT3-NF-κB-TK/GCV suicide gene therapy system. The basal promoter activity of Lenti-CMV-TK and Lenti-STAT3-NF-κB-TK in MDA-MB-231 cells was compared by 3H-FEAU uptake assay. The Lenti-CMV-TK showed ~5 fold higher 3H-FEAU uptake then Lenti -STAT3-NF-κB-TK. In clonogenic assay, cells expressing Lenti-CMV-TK were 2-fold more sensitive to GCV than Lenti-STAT3-NF-κB-TK transduced cells. In vitro effect of STAT3-NF-κB-induced transgene expression was determined by 10ng/mL TNF-α induction and confirmed by western blot analysis and DsRedm fluorescent microscopy. In vivo evaluation of therapeutic effect by bioluminescence and [18F]FHBG microPET imaging indicated that Lenti-STAT3-NF-κB-TK showed more tumor growth retardation than Lenti-CMV-TK when GCV (20 mg/kg) was administered. The invasiveness and expression of cancer stem cell markers were both decreased after STAT3/NF-κB-regulated HSV

  10. Screening for germline mutations in the neurofibromatosis type 2 (NF2) gene in NF2 patients

    Energy Technology Data Exchange (ETDEWEB)

    Andermann, A.A.; Ruttledge, M.H.; Rangaratnam, A. [McGill Univ. and Montreal General Hospital Research Institute, Quebec (Canada)] [and others

    1994-09-01

    Neurofibromatosis type 2 (NF2) is an autosomal dominant disease with over 95% penetrance which predisposes gene carriers to develop multiple tumors of the central nervous system. The NF2 gene is a putative tumor suppressor gene which was previously mapped to the long arm of chromosome 22, and has recently been identified, using positional cloning techniques. The gene encodes a protein, schwannomin (SCH), which is highly homologous to the band 4.1 protein family. In an attempt to identify and characterize mutations which lead to the manifestation of the disease, we have used single strand conformation analysis (SSCA) to screen for germline mutations in all 17 exons of the NF2 gene in 59 unrelated NF2 patients, representing both familial and new mutations. A total of 27 migration abnormalities was found in 26 patients. Using direct sequencing analysis, the majority of these variants were found to result in nonsense, splice-site or frameshift mutations. Mutations identified in familial NF2 patients segregate in the family, and may prove to be useful tools for a simple and direct SSCA-based technique of presymptomatic or prenatal diagnosis in relatives of patients with NF2. This may be of particular importance in children of patients who have new mutations in the NF2 gene, where linkage analysis may not be feasible.

  11. Iso-vector and iso-scalar tensor charges of the nucleon from lattice QCD

    Energy Technology Data Exchange (ETDEWEB)

    Bhattacharya, Tanmoy; Cirigliano, Vincenzo; Gupta, Rajan; Yoon, Boram [Los Alamos National Laboratory, NM (United States). Theoretical Div. T-2; Cohen, Saul D. [Washington Univ., Seattle, WA (United States). Inst. for Nuclear Theory; Joseph, Anosh [Deutsches Elektronen-Synchrotron (DESY), Zeuthen (Germany). John von Neumann-Inst. fuer Computing NIC; Lin, Huey-Wen [California Univ., Berkeley, CA (United States). Dept. of Physics; Collaboration: PNDME Collaboration

    2015-07-15

    We present results for the iso-vector and flavor diagonal tensor charges g{sup u-d}{sub T}, g{sup u}{sub T}, g{sup d}{sub T}, and g{sup s}{sub T} needed to probe novel tensor interactions at the TeV scale in neutron and nuclear β-decays and the contribution of the quark electric dipole moment (EDM) to the neutron EDM. The lattice QCD calculations were done using nine ensembles of gauge configurations generated by the MILC collaboration using the HISQ action with 2+1+1 dynamical flavors. These ensembles span three lattice spacings a∼0.06, 0.09 and 0.12 fm and three quark masses corresponding to the pion masses M{sub π}∼130, 220 and 310 MeV. Using estimates from these ensembles, we quantify all systematic uncertainties and perform a simultaneous extrapolation in the lattice spacing, volume and light quark masses for the connected contributions. The final estimates of the connected nucleon (proton) tensor charge for the iso-vector combination is g{sup u-d}{sub T}=1.020(76) in the MS scheme at 2 GeV. The additional disconnected quark loop contributions needed for the flavor-diagonal matrix elements are calculated using a stochastic estimator employing the truncated solver method with the all-mode-averaging technique. We find that the size of the disconnected contribution is smaller than the statistical error in the connected contribution. This allows us to bound the disconnected contribution and include it as an additional uncertainty in the flavor-diagonal charges. After a continuum extrapolation, we find g{sup u}{sub T}=0.774(66), g{sup d}{sub T}=-0.233(28) and g{sup u+d}{sub T}=0.541(67). The strangeness tensor charge, that can make a significant contribution to the neutron EDM due to the large ratio m{sub s}/m{sub u,d}, is g{sup s}{sub T}=0.008(9) in the continuum limit.

  12. ISO 9000质量体系认证——通向世界的钥匙!%ISO 9000—THE ROAD TO THE WORLD

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ 1 1994版ISO 9000系列标准简介 1.1 ISO 9000系列标准包括的标准范围 ISO 9000系列标准共包括5项标准。它们是: ISO 9000—1:1994《质量管理和质量保证标准第一部分:选择和使用指南》 ISO 9001:1994《质量体系设计、开发、生产、安装和服务的质量保证模式》 ISO 9002:1994《质量体系生产、安装和服务的质量保证模式》 ISO 9003:1994《质量体系最终检验和试验的……

  13. The influenza virus protein PB1-F2 interacts with IKKβ and modulates NF-κB signalling.

    Directory of Open Access Journals (Sweden)

    Ana Luísa Reis

    Full Text Available PB1-F2, a protein encoded by a second open reading frame of the influenza virus RNA segment 2, has emerged as a modulator of lung inflammatory responses but the molecular mechanisms underlying this are only poorly understood. Here we show that PB1-F2 inhibits the activation of NF-κB dependent signalling pathways in luciferase reporter assays. PB1-F2 proteins from four different viruses interact with IKKβ in yeast two-hybrid assays and by co-immunoprecipitation. PB1-F2 expression did not inhibit IKKβ kinase activity or NF-κB translocation into the nucleus, but NF-κB binding to DNA was severely impaired in PB1-F2 transfected cells as assessed by Electrophoretic Mobility Shift Assay. Neither the N-terminal 57 amino acid truncated forms nor the C-terminus of PB1-F2 were able to inhibit NF-κB dependent signalling, indicating that the full length protein is necessary for the inhibition.

  14. The effect of endometritis on level of inflammatory protein and oxidation factor via NF-κB signal pathway

    Institute of Scientific and Technical Information of China (English)

    Ming Ni

    2015-01-01

    Objective:This study explored effect of endometritis induced by LPS on level of inflammatory protein and oxidation factor via NF-κB signal pathway.Methods: Endometial cell was treated with LPS (50, 100, 200 ng/mL). The viability of cell was detected by MTT assay. The concentration of IL-6 and PGE2 was tested by elisa method. The concentration of MDA was tested by thiobarbituric acid method. The concentration of SOD was tested by xanthine oxidation method. The concentration of NO was tested by Gries method. The expression of NF-κB p65 was assayed by western blot.Results:MTT assay demonstrated LPS (50, 100, 00 ng/mL) could suppress cell viability, and the inhibitory effect was highest in 48 h. With the increasing dose of LPS, the activity of SOD decreased, the level of MDA, NO, IL-1 and PGE2 elevated. Finally, LPS stimulated NF-κB p65 phosphorylation.Conclusion:These results suggested endometrial cell treated by LPS make inflammatory factor secreted and anti-oxidant ability decreased, which might be related to NF-κB signal pathway.

  15. Generation and functional characterization of a BCL10-inhibitory peptide that represses NF-kappaB activation.

    Science.gov (United States)

    Marasco, Daniela; Stilo, Romania; Sandomenico, Annamaria; Monti, Simona Maria; Tizzano, Barbara; de Capua, Antonia; Varricchio, Ettore; Liguoro, Domenico; Zotti, Tiziana; Formisano, Silvestro; Ruvo, Menotti; Vito, Pasquale

    2009-08-27

    The molecular complex containing BCL10 and CARMA [CARD (caspase recruitment domain)-containing MAGUK (membrane-associated guanylate kinase)] proteins has recently been identified as a key component in the signal transduction pathways that regulate activation of the transcription factor NF-kappaB (nuclear factor kappaB) in lymphoid and non-lymphoid cells. Assembly of complexes containing BCL10 and CARMA proteins relies on homophilic interactions established between the CARDs of these proteins. In order to identify BCL10-inhibitory peptides, we have established a method of assaying peptides derived from the CARD of BCL10 in binding competition assays of CARD-CARD self-association. By this procedure, a short peptide corresponding to amino acid residues 91-98 of BCL10 has been selected as an effective inhibitor of protein self-association. When tested in cell assays for its capacity to block NF-kappaB activation, this peptide represses activation of NF-kappaB mediated by BCL10, CARMA3 and PMA/ionomycin stimulation. Collectively, these results indicate that residues 91-98 of BCL10 are involved in BCL10 self-association and also participate in the interaction with external partners. We also show that blocking of the CARD of BCL10 may potentially be used for the treatment of pathological conditions associated with inappropriate NF-kappaB activation.

  16. INHIBITORY EFFECTS OF INDOMETHACIN AND MELOXICAM ON NF-κB IN MOUSE PERITONEAL MACROPHAGES%吲哚美辛和美洛昔康对脂多糖诱导的小鼠腹腔 巨噬细胞NF-κB的抑制作用

    Institute of Scientific and Technical Information of China (English)

    胡玉芳; 郭颖; 程桂芳

    2001-01-01

    目的研究非甾体抗炎药吲哚美辛(indomethacin)和美洛昔康(meloxicam)对细菌脂多糖(LPS)诱导表达的C57小鼠腹腔巨噬细胞核转录因子NF-κappaB(NF-κB)的抑制作用。方法 NF-κB的测定采用电泳迁移率改变法。结果小鼠腹腔巨噬细胞经LPS诱导后细胞NF-κB含量明显增高。Indomethacin和meloxicam在10-5-10-7 mol.L-1浓度下可明显降低LPS激活的小鼠腹腔巨噬细胞NF-κB活化。结论 Indomethacin和meloxicam对NF-κB活化的抑制作用可能是两者的抗炎作用机理之一。%AIM To study the inhibitory effects of indomethacin and meloxicam on NF-κB from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice. METHODS NF-κB was measured with the method of electrophoretic mobility shift assay (EMSA). RESULTS After induction by LPS at the concentrations of 1 and 3 μg.mL-1, the NF-κB content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10-7-10-5 mol.L-1, decreased the activation of NF-κB at the concentrations of 1 and 3 μg.mL-1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 μg.mL-1. CONCLUSION The inhibitory effects of indomethacin and meloxicam on NF-κB activation may be one of their mechanisms of antiinflammatory actions.

  17. Styryl-lactone goniothalamin inhibits TNF-α-induced NF-κB activation.

    Science.gov (United States)

    Orlikova, Barbora; Schumacher, Marc; Juncker, Tom; Yan, Choo Chee; Inayat-Hussain, Salmaan H; Hajjouli, Shéhérazade; Cerella, Claudia; Dicato, Mario; Diederich, Marc

    2013-09-01

    (R)-(+)-Goniothalamin (GTN), a styryl-lactone isolated from the medicinal plant Goniothalamus macrophyllus, exhibits pharmacological activities including cytotoxic and anti-inflammatory effects. In this study, GTN modulated TNF-α induced NF-κB activation. GTN concentrations up to 20 μM showed low cytotoxic effects in K562 chronic myelogenous leukemia and in Jurkat T cells. Importantly, at these concentrations, no cytotoxicity was observed in healthy peripheral blood mononuclear cells. Our results confirmed that GTN inhibited tumor necrosis factor-α (TNF-α)-induced NF-κB activation in Jurkat and K562 leukemia cells at concentrations as low as 5 μM as shown by reporter gene assays and western blots. Moreover, GTN down-regulated translocation of the p50/p65 heterodimer to the nucleus, prevented binding of NF-κB to its DNA response element and reduced TNF-α-activated interleukin-8 (IL-8) expression. In conclusion, GTN inhibits TNF-α-induced NF-κB activation at non-apoptogenic concentrations in different leukemia cell models without presenting toxicity towards healthy blood cells underlining the anti-leukemic potential of this natural compound.

  18. In vivo functional analysis of the human NF2 tumor suppressor gene in Drosophila.

    Directory of Open Access Journals (Sweden)

    Heather S Gavilan

    Full Text Available The proper control of tissue growth is essential during normal development and an important problem in human disease. Merlin, the product of the Neurofibromatosis 2 tumor suppressor gene, has been extensively studied to understand its functions in growth control. Here we describe experiments in which we used Drosophila as an in vivo system to test the functions of the normal human NF2 gene products and patient-derived mutant alleles. Although the predominant NF2 gene isoform, isoform 1, could functionally replace the Drosophila Merlin gene, a second isoform with a distinct C-terminal tail could not. Immunofluorescence studies show that the two isoforms have distinct subcellular localizations when expressed in the polarized imaginal epithelium, and function in genetic rescue assays correlates with apical localization of the NF2 protein. Interestingly, we found that a patient-derived missense allele, NF2L64P, appears to be temperature sensitive. These studies highlight the utility of Drosophila for in vivo functional analysis of highly conserved human disease genes.

  19. ISO 26000融入图书馆实践的思考%Integration of ISO 26000 into Library Practices

    Institute of Scientific and Technical Information of China (English)

    刘川; 夏红兵

    2014-01-01

    图书馆的决策和活动能够影响社会和环境,是图书馆承担社会责任的缘由.ISO 26000拓展了图书馆社会责任理论与实践的领域.详述ISO 26000各条款内容,阐述其对图书馆社会责任发展的意义.指出图书馆应从宣传教育、制定图书馆社会责任指南、加强规章体系建设等方面全面融入ISO 26000,以推动图书馆的社会责任实践.

  20. 2000版ISO 9000族标准的修订与转换实施%Modification and implementation of ISO 9000:2000

    Institute of Scientific and Technical Information of China (English)

    张丽生

    2001-01-01

    @@1 2000版ISO 9000族标准修订过程 2000版ISO 9000族标准(2000版ISO 9000、ISO 9001和ISO 9004三个国际标准已经各成员机构表决并获通过)已于2000年12月15日正式发布。该标准族的修订大致经历如下过程。 国际标准化组织(英文缩写为ISO)分别于1986年发布ISO 8402《质量——术语》和1987年发布ISO 9000《质量管理和质量保证标准——选择和使用指南》、ISO 9001《质量体系——设计开发、生产、安装和服务的质量保证模式》、ISO 9002《质量体系——生产、安装和服务的质量保证模式》、ISO 9003《质量体系——最终检验和试验的质量保证模式》、ISO 9004《质量管理和质量体系要素——指南》等6项国际标准,通称为ISO 9000系列标准或称为1987版ISO 9000系列国际标准。

  1. Difference and Sameness of ISO9001, CMM and SPICE%试析ISO 9001 CMM SPICE之异同

    Institute of Scientific and Technical Information of China (English)

    李晓桦

    2002-01-01

    @@ 1 ISO9001、CMM、SPICE概述 随着计算机技术的飞速发展,对软件的需求急剧增长.为了使软件开发适应形势的需要,20世纪90年代前后,国际上出现了一些可用于软件管理的质量管理标准和软件过程模型.其中,ISO9001、CMM、SPICE使用最广泛、影响最大.

  2. Requerimientos establecidos por las normas ISO para papeles permanentes Established requirements for ISO standards for permanent papers

    Directory of Open Access Journals (Sweden)

    Juan Facundo Araujo

    2011-06-01

    Full Text Available El patrimonio documental nacional e internacional se encuentra en su gran mayoría en soporte papel. A partir de la industrialización en la manufactura de dicho material se ha producido papel con niveles de acidez, que sumado a otros factores, ha sido causante del deterioro de este tipo de patrimonio documental. En las últimas décadas, con la incorporación de nuevos procesos dentro de la producción papelera se ha resuelto en parte dicha problemática. El organismo internacional de normalización ISO ha redactado dos normas al respecto: la norma ISO 9706 e ISO 11108. Con la difusión y recomendación de uso del papel permanente para la conservación del patrimonio documental a largo plazo, se hace imprescindible conocer dicha normativa e informarse al respecto. En el presente artículo se describen las variables en la fabricación del papel que influyen en la permanencia de los papeles. También se determinan los conceptos y antecedentes que rodean ambas normas ISO, así como las características principales y partes que componen las mismas. Como anexo se consigna un glosario con los principales términos técnicos utilizados.Most of the national and international documentary heritage are on paper. From industrialization in the manufacture of such material, paper with acidity levels has been produced which, together with other factors, has been causing the deterioration of this type of documentary heritage. In recent decades, with the incorporation of new paper production processes this problem has been partly solved. The international standards organism ISO has drafted two rules in this connection: ISO 9706 and ISO 11108. With the spread and recommendation for the use of permanent paper for preservation of documentary heritage in the long term, it is essential to know the standards and be well informed. This article describes the variables in the manufacture of paper that influence the permanence of the papers. It also identifies the

  3. Interpretation of the New Version of ISO 21461%新版ISO 21461标准解读

    Institute of Scientific and Technical Information of China (English)

    洪颖; 艾峰; 李瑜; 何重辉; 曹丽华

    2013-01-01

    本文主要介绍了ISO 21461-2012《橡胶--硫化橡胶化合物中油的芳香性测定》标准的背景及适用范围,选取重点并结合检测实际对新版标准进行解读。%This paper mainly introduces the background and application scope of ISO 21461-2012 Rubber-Determination of the aromaticity of oil in vulcanized rubber compounds, and interpreted on the focus with the new standard.

  4. Proteomic Analyses of NF1-Interacting Proteins in Keratinocytes

    Science.gov (United States)

    2015-04-01

    AWARD NUMBER: W81XWH-14-1-0070 TITLE: Proteomic Analyses of NF1 -Interacting Proteins in Keratinocytes PRINCIPAL INVESTIGATOR: Shyni Varghese...TITLE AND SUBTITLE 5a.CONTRACT NUMBER Proteomic Analyses of NF1 -Interacting Proteins in Keratinocytes 5b. GRANT NUMBER W81XWH-14-1-0070 5c. PROGRAM...in the NF1 null epidermis, we analyzed NF1 expression in a mouse model of psoriasis (imiquimod-induced psoriasis-like skin inflammation) and

  5. New water and remote galaxies complete ISO's observations

    Science.gov (United States)

    ISO's operational teams at ESA's ground station at Villafranca near Madrid have been hurrying to provide the world's astronomers with as many observations as possible. They have long anticipated the exhaustion of ISO's vital supply of liquid helium, which cooled the infrared telescope and its instruments to their operating temperatures, close to absolute zero. Two weeks after ISO was put into orbit on 17 November 1995 by an Ariane 44P launcher, the external parts of the cooling system had settled to the operating temperature. The specification required that ISO should then operate for at least 18 months -- implying that operations might have to end in May 1997. Thanks to superb engineering by European industry, which built the spacecraft and its super-cool telescope, ISO has given astronomers almost a year longer than that. During the extra time the count of ISO's observations of cosmic objects has risen from 16,000 to about 26,000. Among the benefits of ISO's longevity has been the chance to examine an important region of the sky, in and around the constellation of Orion. This was not accessible in the nominal mission but has now been observed in two periods. Four inter national teams, supported by national funding agencies, supplied the instruments to analyse the infrared rays received by ISO's telescope. The principal investigators leading the teams are Dietrich Lemke (Heidelberg, Germany) for the versatile photometer ISOPHOT, Catherine Cesarsky (Saclay, France) for the camera ISOCAM, Thijs de Graauw (Groningen, the Netherlands) for the Short Wavelength Spectrometer SWS, and Peter Clegg (London, UK) for the Long Wavelength Spectrometer LWS. Water vapour on Titan A big difference between ISO and the only previous infrared astronomy satellite (IRAS 1983) has been its ability to examine individual objects across a wide range of accurately defined infrared wavelengths. Many spectra showing patterns of intensities at the different wavelengths have enabled astronomers

  6. Launching the chaotic realm of iso-fractals: A short remark

    Energy Technology Data Exchange (ETDEWEB)

    O' Schmidt, Nathan [Department of Mathematics, Boise State University, 1910 University Drive, Boise, ID 83725 (United States); Katebi, Reza [Department of Physics, California State University in Fullerton, 800 North State College Boulevard, Fullerton, CA 92831 (United States); Corda, Christian [Institute for Theoretical Physics and Advanced Mathematics Einstein-Galilei (IFM), Via Santa Gonda 14, 59100 Prato (Italy)

    2015-03-10

    In this brief note, we introduce the new, emerging sub-discipline of iso-fractals by highlighting and discussing the preliminary results of recent works. First, we note the abundance of fractal, chaotic, non-linear, and self-similar structures in nature while emphasizing the importance of studying such systems because fractal geometry is the language of chaos. Second, we outline the iso-fractal generalization of the Mandelbrot set to exemplify the newly generated Mandelbrot iso-sets. Third, we present the cutting-edge notion of dynamic iso-spaces and explain how a mathematical space can be iso-topically lifted with iso-unit functions that (continuously or discretely) change; in the discrete case examples, we mention that iteratively generated sequences like Fibonacci’s numbers and (the complex moduli of) Mandelbrot’s numbers can supply a deterministic chain of iso-units to construct an ordered series of (magnified and/or de-magnified) iso-spaces that are locally iso-morphic. Fourth, we consider the initiation of iso-fractals with Inopin’s holographic ring (IHR) topology and fractional statistics for 2D and 3D iso-spaces. In total, the reviewed iso-fractal results are a significant improvement over traditional fractals because the application of Santilli’s iso-mathematics arms us an extra degree of freedom for attacking problems in chaos. Finally, we conclude by proposing some questions and ideas for future research work.

  7. NF-KB downregulation may be involved the depression of tumor cell proliferation mediated by human mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    Ling QIAO; Tie-jun ZHAO; Feng-ze WANG; Chang-liang SHAN; Li-hong YE; Xiao-dong ZHANG

    2008-01-01

    Aim:It has been reported that stem cells are able to home to tumorigenesis and inhibit the proliferation of tumor cells.The purpose of our study was to demon-strate the molecular mechanism of the inhibitory proliferation of hepatoma cells and breast cancer cells mediated by human mesenchymal stem cells (hMSCs).Methods:The proliferation of H7402 human hepatoma cells and MCF-7 human breast cancer cells was measured by the 5-bromodeoxyuridine (BrdU) incorpora-tion assay and flow cytometry assay after the treatment with conditioned media from hMSCs culture,such as Z3 cells or BMMS-03 cells.The role of NF-kB or the phosphorylation of inhibitor kBoα (p-IkBα) in the depression of hepatoma or breast cancer cells treated with conditioned media from Z3 cells or BMMS-03 cells was examined by reporter gene assay,quantitative real-time PCR,and Western blot analysis,respectively.Results:The proliferation of H7402 cells and MCF-7 cells was decreased significantly by the BrdU incorporation assay and flow cytometry assay after treatment.The transcriptional activity and mRNA level of NF-kB were downregulated in the treated cells by reporter gene assay and quantitative real-time PCR in a dose-dependent manner.At the protein level,NF-kB and p-IkBα decreased in the treated cells by Western blot analysis.Conclusion:Conditioned media from hMSCs are able to inhibit the proliferation of tumor cells.NF-kB downregulation is one of reasons for the depression of tumor cell proliferation mediated by hMSCs.

  8. ISO 9001:2000--setting the standard for quality management.

    Science.gov (United States)

    Edelstein, M E

    2001-04-01

    How will you approach the next quality management initiative? This article offers an introduction to the ISO 9001:2000 quality management system standards that can be applied to the HIM industry and how these standards can improve your department's policies and procedures.

  9. Re-engineering caused by ISO-9000 certification

    DEFF Research Database (Denmark)

    Hvam, Lars; Nielsen, Anders Paarup; Bjarnø, Ole-Christian

    1997-01-01

    Based on a project performed at a medium-sized producer of medical utensils, reviews some of the problems which the company experienced in connection with the system built up during ISO 9001 certification, and the re-engineering efforts which were performed in order to relieve these problems...

  10. An ISO and IUE study of planetary nebula NGC 2440

    NARCIS (Netherlands)

    Salas, JB; Pottasch, [No Value; Feibelman, WA; Wesselius, PR

    2002-01-01

    The infrared and ultraviolet spectra of planetary nebula NGC2440 are presented. The observations were made by the Infrared Space Observatory (ISO) and the International Ultraviolet Explorer (IUE). These data, in conjunction with published optical observations have been used to derive electron temper

  11. First Results of ISO-SWS Grating Observations of Saturn

    NARCIS (Netherlands)

    de Graauw, Th.; Encrenaz, Th.; Schaeidt, S.; Lellouch, E.; Feuchtgruber, H.; Beintema, D. A.; Bezard, B.; Drossart, P.; Griffin, M.; Heras, A.; Kessler, M.; Leech, K.; Morris, A.; Roelfsema, P. R.; Roos-Serote, M.; Salama, A.; Vandenbussche, B.; Valentijn, E. A.; Davies, G. R.; Naylor, D. A.

    1996-01-01

    The spectrum of Saturn has been recorded between 2.3 and 15 mu m, on June 13, 1996, with the grating mode of the Short-Wavelength Spectrometer of ISO (Infrared Space Observatory). The resolving power is about 1500 and the sensitivity is better than 1 Jy. As compared to Jupiter, the spectrum of Satur

  12. Iso-geometric shape optimization of magnetic density separators

    DEFF Research Database (Denmark)

    Dang Manh, Nguyen; Evgrafov, Anton; Gravesen, Jens;

    2014-01-01

    covers with B-splines and defines a cost functional that measures the non-uniformity of the magnetic field in an area above the poles. The authors apply an iso-geometric shape optimization procedure, which allows us to accurately represent, analyze and optimize the geometry using only a few design...

  13. Mid-infrared ISO spectroscopy of NGC 4945

    NARCIS (Netherlands)

    Spoon, HWW; Koornneef, J; Moorwood, AFM; Lutz, D; Tielens, AGGM

    2000-01-01

    We have observed the central region of the nearby starburst galaxy NGC 4945 with the mid-infrared spectrometers SWS and PHT-S aboard ISO. We do not find any evidence for the existence of the powerful AGN, inferred from hard X-ray observations. The upper limits on our AGN tracers [Nev]14.32 mu m&24.3

  14. The Relative Spectral Response Calibration of the ISO-SWS

    NARCIS (Netherlands)

    Vandenbussche, B.; Morris, P. W.; Valentijn, E. A.; Beintema, D. A.; Boxhoorn, D. R.; Decin, L.; de Graauw, T.; Feuchtgruber, H.; Heras, A. M.; Lahuis, F.; Lorente, R.; Lutz, D.; Roelfsema, P. R.; Salama, A.; Shipman, R. F.; van Malderen, R.; Wieprecht, E.

    2001-01-01

    The Relative Spectral Response Function (RSRF) characterises the wavelength dependent response of a spectrometer. The wavelength range of the grating section of the ISO-SWS is covered in 15 spectral bands for each of which the RSRF has to be determined. We discuss how the RSRF was measured during th

  15. ISO observations of Titan with SWS/grating

    Science.gov (United States)

    Coustenis, A.; Encrenaz, T.; Salama, A.; Lellouch, E.; Gautier, D.; Kessler, M. F.; deGraauw, T.; Samuelson, R. E.; Bjoraker, G.; Orton, G.

    1997-01-01

    The observations of Titan performed by the Infrared Space Observatory (ISO) short wavelength spectrometer (SWS), in the 2 micrometer to 45 micrometer region using the grating mode, are reported on. Special attention is given to data from Titan concerning 7 micrometer to 45 micrometer spectral resolution. Future work for improving Titan's spectra investigation is suggested.

  16. Information Asymmetries as Trade Barriers: ISO 9000 Increases International Commerce

    Science.gov (United States)

    Potoski, Matthew; Prakash, Aseem

    2009-01-01

    Spatial, cultural, and linguistic barriers create information asymmetries between buyers and sellers that impede international trade. The International Organization for Standardization's ISO 9000 program is designed to reduce these information asymmetries by providing assurance about the product quality of firms that receive its certification.…

  17. The hidden networks of knowledge in ISO 14001

    DEFF Research Database (Denmark)

    Jørgensen, Ulrik

    2003-01-01

    The ISO 14001 standard is defined as a management standard based on an assumed existing knowledge base of what is to be considered being environmental problems. In the implementation of the standard is become evident, that its use and impact is entirely dependent of the existence of networks...

  18. ISO19770-1:2012 SAM process guidance

    CERN Document Server

    Canavan, Rory

    2012-01-01

    The pocket guide offers a concise summary of the principles of software asset management as conveyed by ISO 19770-1: 2012, and provides advice and guidance on how to kick-start your own SAM programme - something the Standard alone doesn't offer.

  19. First results of ISO-SWS observations of Jupiter

    NARCIS (Netherlands)

    Encrenaz, T.; de Graauw, T.; Schaeidt, S.; Lellouch, E.; Feuchtgruber, H.; Beintema, D. A.; Bezard, B.; Drossart, P.; Griffin, M.; Heras, A.; Kessler, M.; Leech, K.; Morris, P.; Roelfsema, P. R.; Roos-Serote, M.; Salama, A.; Vandenbussche, B.; Valentijn, E. A.; Davis, G. R.; Naylor, D. A.

    1996-01-01

    The spectrum of Jupiter has been recorded between 2.75 and 14.5 mu m with the grating mode of the Short-Wavelength Spectrometer (SWS) of ISO. The resolving power is 1500. The main preliminary results of this observation are (1) at 3 mu m, the first spectroscopic signature, probably associated with N

  20. Choosing change strategy for ISO/IEC 33014

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Johansen, Jørn

    2015-01-01

    The ISO/IEC 33014.2013 [1] standard on process improvement includes a core activity called ‘identify the overall change strategy’, which includes selecting a change strategy among the many available. This selection can be carried out using the ImprovAbility model [2] in which there is a framework...

  1. Quality in Service Management System According to ISO 20000

    Science.gov (United States)

    Šrubařová, Ružena

    2012-12-01

    ISO 20000 is a service management system standard. It specifies requirements for the service provider to plan, establish, implement, operate, monitor, review, maintain and improve an service management system. The requirements include the design, transition, delivery and improvement of services to fulfil agreed service requirements.

  2. The ISO 5-pass authentication in χ-Spaces

    DEFF Research Database (Denmark)

    Cáccamo, Mario J.; Crazzolara, Federico; Milicia, Giuseppe

    2002-01-01

    We implement the ISO 5-pass authentication protocol and study its correctness using the χ-Space framework. χ-Spaces is a novel domain specific programming language designed for developing protocols and in particular security protocols. χ-Spaces programming language is an implementation of SPL...

  3. Study on Extraction Performance of Tri-iso-amyl Phosphate

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The distribution performance of HNO3, Pu(Ⅲ ), Pu(Ⅳ) and Np(Ⅳ) in the two phase system of Tri-iso-Amyl Phosphate(TiAP) and aqueous, the influence of the concentration of extractant, nitric acid and Al(NO3)3 on the distribution ratio of Pu(Ⅲ ),

  4. ISO/IEC 38500 the IT governance standard

    CERN Document Server

    Calder, Alan

    2008-01-01

    The emergence of ISO/IEC 38500 - the international standard for the corporate governance of information and communication technology - puts boards around the world in a position from which they can take effective action to apply core governance principles to their information and communication technology.

  5. IMPLEMENTING AN ISO 10001-BASED PROMISE IN INPATIENTS CARE

    Directory of Open Access Journals (Sweden)

    Mohammad Ashiqur Rahman Khan

    2013-09-01

    Full Text Available This paper presents the implementation of a Customer Satisfaction Promise (CSP that requires nurses to introduce themselves and explain the care plan to the patients of a hospital unit in Canada. The CSP implementation, maintenance and improvement activities were based on ISO 10001:2007. Qualitative and quantitative performance data were collected from nurses, the unit manager and patients, and improvement suggestions were made. During the implementation, nurses introduced themselves 95% of the time and explained the care plan 86% of the time. When interviewed, some nurses stated that the CSP was a good reinforcement of a practice already expected of them, which made patients happy, satisfied and more comfortable. Data from a small sample of patients was not adequate in clearly indicating the CSP's performance or improvement, but was useful in validating the survey and the feedback form. To our knowledge, applications of ISO 10001:2007 in health care have not been studied. Furthermore, this paper may be the first example of the integrated use of ISO 10001 and ISO 10002 in health care.

  6. The NF1 gene revisited - from bench to bedside.

    Science.gov (United States)

    Yap, Yoon-Sim; McPherson, John R; Ong, Choon-Kiat; Rozen, Steven G; Teh, Bin-Tean; Lee, Ann S G; Callen, David F

    2014-08-15

    Neurofibromatosis type 1 (NF1) is a relatively common tumour predisposition syndrome related to germline aberrations of NF1, a tumour suppressor gene. The gene product neurofibromin is a negative regulator of the Ras cellular proliferation pathway, and also exerts tumour suppression via other mechanisms. Recent next-generation sequencing projects have revealed somatic NF1 aberrations in various sporadic tumours. NF1 plays a critical role in a wide range of tumours. NF1 alterations appear to be associated with resistance to therapy and adverse outcomes in several tumour types. Identification of a patient's germline or somatic NF1 aberrations can be challenging, as NF1 is one of the largest human genes, with a myriad of possible mutations. Epigenetic factors may also also contribute to inadequate levels of neurofibromin in cancer cells. Clinical trials of NF1-based therapeutic approaches are currently limited. Preclinical studies on neurofibromin-deficient malignancies have mainly been on malignant peripheral nerve sheath tumour cell lines or xenografts derived from NF1 patients. However, the emerging recognition of the role of NF1 in sporadic cancers may lead to the development of NF1-based treatments for other tumour types. Improved understanding of the implications of NF1 aberrations is critical for the development of novel therapeutic strategies.

  7. The role of NF-kappaB in endometriosis.

    Science.gov (United States)

    Kaponis, Apostolos; Iwabe, Tomio; Taniguchi, Fuminori; Ito, Masayuki; Deura, Imari; Decavalas, George; Terakawa, Naoki; Harada, Tasuku

    2012-06-01

    The nuclear factor kappaB (NF-kappaB) is a ubiquitously expressed transcription factor playing vital roles in innate immunity and other processes involving cellular survival, proliferation, and differentiation. This review highlights the importance of NF-kappaB in the pathophysiology of endometriosis. Constitutive activation of NF-kappaB has been shown in endometriotic lesions. Complex interactions of NF-kappaB with steroid receptors and apoptotic molecules in endometriosis resulting in opposing roles of NF-kappaB are discussed. NF-kappaB regulates the expression of cytokines mediating autocrine self-amplifying cycles of cytokine release and NF-kappaB activation, leading to maintenance of inflammatory reactions in endometriosis. NF-kappaB can contribute to the increased ability of endometriotic cells to invade and adhere to the peritoneal surface by regulating the expression of matrix metaloproteinases. We are presenting the role of NF-kappaB to regulate vascularization and oxidative stress in endometriotic cells. Effects of drugs used for the treatment of endometriosis on NF-kappaB pathway are presented and we show how drugs that inhibit the NF-kappaB can mediate the progression of endometriosis. Novel therapeutic strategies involving the NF-kappaB and applied in endometriosis are also discussed.

  8. 42 CFR 483.122 - FFP for NF services.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false FFP for NF services. 483.122 Section 483.122 Public... Annual Review of Mentally Ill and Mentally Retarded Individuals § 483.122 FFP for NF services. (a) Basic...(e)(7)(E) of the Act, FFP is available in State expenditures for NF services provided to a...

  9. Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells.

    Science.gov (United States)

    Assar, Emelia A; Vidalle, Magdalena Castellano; Chopra, Mridula; Hafizi, Sassan

    2016-07-01

    We studied the effect of the potent dietary antioxidant lycopene on multiple points along the nuclear factor kappa B (NF-κB) signaling pathway in prostate and breast cancer cells. Lycopene significantly inhibited prostate and breast cancer cell growth at physiologically relevant concentrations of ≥1.25 μM. Similar concentrations also caused a 30-40 % reduction in inhibitor of kappa B (IκB) phosphorylation in the cells, as determined by western blotting. Furthermore, the same degree of inhibition by lycopene was observed for NF-κB transcriptional activity, as determined by reporter gene assay. Concomitant with this, immunofluorescence staining of lycopene-treated cells showed a significant suppression (≥25 %) of TNF-induced NF-κB p65 subunit nuclear translocation. Further probing of lycopene's effects on upstream elements of the NF-κB pathway showed a 25 % inhibition of both activity of recombinant IκB kinase β (IKKβ) kinase in a cell-free in vitro assay, as well as activity of IKKβ immunoprecipitated from MDA-MB-231 cells treated with lycopene. In conclusion, the anticancer properties of lycopene may occur through inhibition of the NF-κB signaling pathway, beginning at the early stage of cytoplasmic IKK kinase activity, which then leads to reduced NF-κB-responsive gene regulation. Furthermore, these effects in cancer cells were observed at concentrations of lycopene that are relevant and achievable in vivo.

  10. DNA Damage-Induced NF-κB Activation in Human Glioblastoma Cells Promotes miR-181b Expression and Cell Proliferation

    Directory of Open Access Journals (Sweden)

    Rui-Xue Xu

    2015-01-01

    Full Text Available Background: Glioblastoma (GBM is the most common and most aggressive form of brain cancer. After surgery, radiotherapy is the mainstay of treatment for GBM patients. Unfortunately, the vast majority of GBM patients fail responding to radiotherapy because GBM cells remain highly resistant to radiation. Radiotherapy-induced DNA damage response may correlate with therapeutic resistance. Methods: Ionizing radiation (IR was used to induce DNA damage. Cell proliferation and migration were detected by wound-healing, MTT and apoptosis assays. Dual-luciferase assays and Western blot analysis were performed to evaluate NF-κB activation and validate microRNA targets. Real-time PCR was used to study mRNA and microRNA levels. Results: IR-induced DNA damage activated NF-κB in GBM cells which promoted expression of IL-6, IL-8 and Bcl-xL, thereby contributing to cell survival and invasion. Knockdown SENP2 expression enhanced NF-κB essential modulator (NEMO SUMOylation and NF-κB activity following IR exposure. miR-181b targets SENP2 and positively regulated NF-κB activity. Conclusion: NF-κB activation by DNA damage in GBM cells confers resistance to radiation-induced death.

  11. Foam-stabilizing effects and cling formation patterns of iso-alpha-acids and reduced iso-alpha-acids in lager beer.

    Science.gov (United States)

    Kunimune, Takeshi; Shellhammer, Thomas H

    2008-09-24

    Foam-stabilizing properties and cling formation patterns of iso-alpha-acids and reduced iso-alpha-acids were investigated using an unhopped lager beer. Unhopped beer was dosed with iso-alpha-acid (Iso), rho-iso-alpha-acid (Rho), tetrahydro-iso-alpha-acid (Tetra), and hexahydro-iso-alpha-acid (Hexa), separately, over a range of concentrations from 2 to 10 ppm. A uniform foam was created by Inpack 2000 Flasher Head and was measured by a NIBEM Foam Stability Tester (NIBEM-TPH) followed by a NIBEM Cling Meter (NIBEM-CLM) to determine the relationship between the concentration and NIBEM-30 and the cling formation ability of each compound. The foam-stabilizing power was determined to be Tetra, Hexa, Iso, and Rho from the strongest to weakest. Linear regression models were created using the NIBEM-TPH data set, and on the basis of 95% confidence intervals, the foam stability of Tetra or Hexa became significantly larger than that of Iso when 2.4 or 4.2 ppm of Tetra or Hexa was used as a replacement for Iso, respectively. Cling formation patterns could be categorized into three groups: "ring", "mesh", and "powdery". The control beer had the lowest foam stability and did not yield any foam cling.

  12. NF-κB signalling requirement for brain myelin formation is shown by genotype/MRI phenotype correlations in patients with Xq28 duplications.

    Science.gov (United States)

    Philippe, Orianne; Rio, Marlène; Malan, Valérie; Van Esch, Hilde; Baujat, Geneviève; Bahi-Buisson, Nadia; Valayannopoulos, Vassili; Gesny, Roseline; Bonnefont, Jean-Paul; Munnich, Arnold; Froyen, Guy; Amiel, Jeanne; Boddaert, Nathalie; Colleaux, Laurence

    2013-02-01

    One of the key signals regulating peripheral myelin formation by Schwann cell is the activation of the transcription factor NF-κB. Yet, whether NF-κB exerts similar functions in central myelin formation by oligodendrocytes remains largely unknown. We previously reported white matter abnormalities with unusual discordance between T2 and FLAIR sequences in a patient with intellectual disability and defective NF-κB signalling. These observations prompted us to hypothesise that NF-κB signalling may have a role in the axon myelination process of central neurons. We report here on five male patients with Xq28 duplications encompassing MECP2, three of which presented white matter anomalies on brain MRI. Array-CGH and FISH analyses demonstrated that brain abnormalities correlate with additional copies of the IKBKG, a gene encoding a key regulator of NF-κB activation. Quantitative RT-PCR experiments and κB-responsive reporter gene assays provide evidence that IKBKG overexpression causes impaired NF-κB signalling in skin fibroblasts derived from patients with white matter anomalies. These data further support the role of NF-κB signalling in astroglial cells for normal myelin formation of the central nervous system.

  13. [Efforts to achieve and effects of acquiring ISO 15189 in Tokushima University Hospital].

    Science.gov (United States)

    Shono, Kazuko; Kishi, Misako; Satou, Mituyo; Nagamine, Yasunori; Doi, Tosio

    2009-12-01

    The medical laboratory of Tokushima University Hospital acquired ISO 15189, an international standard for medical laboratories, on July 6th, 2007, resulting in it achieving the 24th place in Japan and 5th place among national university hospitals. The first surveillance was just performed on October 6th, 2008. Tokushima University Hospital, in which our medical laboratory is included as one section, already succeeded in acquiring ISO 9001, PrivacyMark System, and Quality Health Care ver. 5 before accomplishing ISO 15189. To achieve ISO 15189, we prepared documents based on ISO 9001 without any consultation, resulting in a review of the difference between ISO 9001 and ISO 15189 after the preliminary survey. Although achieving ISO 15189 resulted in an improvement in the reliability of laboratory results and accuracy, leading to the development of our technical skills and awareness, and sharing of knowledge, we consider that the considerable investment of time to prepare the requirements remains to be overcome.

  14. 78 FR 23940 - Use of International Standard ISO-10993, “Biological Evaluation of Medical Devices Part 1...

    Science.gov (United States)

    2013-04-23

    ... HUMAN SERVICES Food and Drug Administration Use of International Standard ISO-10993, ``Biological... International Standard ISO-10993, `Biological Evaluation of Medical Devices Part 1: Evaluation and Testing... entitled ``Use of International Standard ISO-10993, `Biological Evaluation of Medical Devices Part...

  15. Signal transduction mechanisms involved in S100A4-induced activation of the transcription factor NF-κB

    Directory of Open Access Journals (Sweden)

    Mælandsmo Gunhild M

    2010-05-01

    Full Text Available Abstract Background The metastasis-promoting protein S100A4 activates the transcription factor NF-κB through the classical NF-κB activation pathway. The upstream signal transduction mechanisms leading to increased NF-κB activity are, however, incompletely characterized. Methods The human osteosarcoma cell line II-11b was stimulated with recombinant S100A4 in the presence or absence of inhibitors of common signal transduction pathways, and NF-κB activity was examined using a luciferase-based reporter assay and phosphorylation of IκBα. mRNA expression was analyzed by real-time RT-PCR, protein expression was examined by Western blotting and IKK activity was measured using an in vitro kinase assay. The role of upstream kinases and the cell surface receptor RAGE was investigated by overexpression of dominant negative proteins and by siRNA transfection. Results The Ser/Thr kinase inhibitors H-7 and staurosporine inhibited S100A4-induced IκBα phosphorylation and subsequent NF-κB activation. The protein tyrosine kinase inhibitor genistein and the phospholipase C inhibitor compound 48/80 had a partial inhibitory effect on IκBα phosphorylation, whereas inhibitors of protein kinase C, G-protein coupled receptors and PI 3-kinases had no effect on the level of phosphorylation. Interestingly, S100A4 treatment induced activating phosphorylations of IKKα/β, but neither H-7 nor staurosporine was able to significantly inhibit IKK activation. Dominant negative MEKK1 or NIK did not inhibit S100A4-induced NF-κB activity, and S100A4 stimulation did not influence AKT phosphorylation. Furthermore, diminished expression of the putative S100 protein receptor RAGE did not affect the observed phosphorylation of IκBα. Conclusions S100A4 activates NF-κB by inducing phosphorylation of IKKα/β, leading to increased IκBα phosphorylation. The Ser/Thr kinase inhibitors H-7 and staurosporine attenuated S100A4-induced NF-κB activation and inhibited IKK

  16. Cosmic water traced by Europe's space telescope ISO

    Science.gov (United States)

    1996-05-01

    In retracing this history, ISO also observes water in the form of ice in cooler regions around the stars, and in the dust surrounding young stars, from which planets could evolve. Comets represent an intermediate stage in planet-building, and they contain much water ice. According to one hypothesis the newly formed Earth received some of its water directly from impacting comets. Water vapour in the Earth's atmosphere has prevented telescopes on the ground from detecting the water vapour among the stars, except in very unusual circumstances. ISO orbiting in space escapes the impediment of the atmosphere. Excellent onboard instruments register the characteristic infrared signatures of water vapour, water ice and many other materials. When ISO scrutinizes selected objects, it detects emissions or absorptions of infrared rays at particular wavelengths, or "lines" in a spectrum, which reveal the presence of identifiable atoms, molecules and solids. The Short Wavelength Specrometer and the Long Wavelength Spectrometer provide detailed chemical diagnoses, and the photometer ISOPHOT and camera ISOCAM also have important spectroscopic capabilities. Examples of water detection were among many topics reviewed at the First ISO Science Workshop held at ESA's Research and Technology Centre (ESTEC) in Noordwijk, the Netherlands (29-31 May) when 300 astronomers from Europe, the USA and Japan gathered to assess results from ISO since its launch on 17 November 1995. The Long Wavelength Spectrometer has made remarkable observations of water-vapour lines in the vicinity of dying stars and in star-forming regions. So has the Short Wavelength Spectrometer, which also detects water ice. The photometer lSOPHOT has registered water ice in a large number of objects. Although fascinated by the natural history of water in the cosmos, astronomers have more technical reasons for welcoming ISO's observations. They can use thc details in a spectrum to reduce the abundance of water and its

  17. New Standard ISO 9001:2015 and its Effect on Organisations

    OpenAIRE

    Srđan Medić; Biljana Karlović; Zrinko Cindrić

    2016-01-01

    ISO 9001 is the international standard which specifies requirements for quality management systems (QMS). Organisations implement requirements of the standard to demonstrate the ability to consistently provide products and services that meet customer and regulatory requirements. It is the most popular standard of the ISO 9000 series and the only standard in the series to which organisations can certify. The new version of ISO 9001 was released in September 2015 and changes made in ISO 900...

  18. Pro-apoptotic role of NF-κB pathway inhibition in lipopolysaccharide-stimulated polymorphonuclear neutrophils

    Institute of Scientific and Technical Information of China (English)

    刘艳梅; 张君岚; 赵占胜; 凌亦凌

    2003-01-01

    Objective To investigate the role of nuclear factor kappa B (NF-κB) pathway inhibition in lipopolysaccharide (LPS)-stimulated apoptosis of polymorphonuclear neutrophils (PMNs).Methods Rats with acute lung injury induced by LPS intratracheal instillation and cultured human venous PMNs were studied. Pyrrolidine dithiocarbamate (PDTC) and gliotoxin were used as NF-κB inhibitors. Additionally, to explore the role of extracellularly regulated protein kinase as an upstream signal in NF-κB pathway on regulating LPS-stimulated PMN apoptosis, PD098059, the specific inhibitor of extracellularly regulated protein kinase, was also applied. The lung injury was determined by protein content and PMN numbers in bronchoalveolar lavage fluid. PMN apoptosis was measured by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) end labeling and DNA fragmentation. IκBα degradation was analyzed by Western blot. NF-κB DNA binding activity was detected by an electrophoretic mobility shift assay.Results (1) The increase of protein content and PMN numbers in bronchoalveolar lavage fluid induced by LPS (100 μg per rat) intratracheal instillation were alleviated by PDTC (50, 100, or 200 mg/kg, I.p.) in a dose-dependent manner. (2) PMNs apoptosis in vivo or in vitro was delayed by LPS, and accelerated by PDTC, gliotoxin or PD098059 pretreatment. (3) IκBα degradation and increased NF-κB DNA binding activity mediated by LPS were inhibited by PDTC, gliotoxin or PD098059 pretreatment.Conclusion Inhibition of either NF-κB itself or the upstream signals in NF-κB pathway such as extracellularly regulated protein kinases has therapeutic effect on LPS-induced acute lung injury, in which the dysregulation of PMN apoptosis plays an important role.

  19. NOTCH1 can initiate NF-kappaB activation via cytosolic interactions with components of the T cell signalosome

    Directory of Open Access Journals (Sweden)

    Lisa M Minter

    2014-05-01

    Full Text Available T cell stimulation requires the input and integration of external signals. Signaling through the T cell receptor (TCR is known to induce formation of the membrane-tethered CBM complex, comprising CARMA1, BCL10 and MALT1, which is required for TCR-mediated NF-kappaB activation. TCR signaling has been shown to activate NOTCH proteins, transmembrane receptors also implicated in NF-kappaB activation. However, the link between TCR-mediated NOTCH signaling and early events leading to induction of NF-kappaB activity remains unclear. In this report, we demonstrate a novel cytosolic function for NOTCH1 and show that it is essential to CBM complex formation. Using a model of skin allograft rejection, we show in vivo that NOTCH1 acts in the same functional pathway as PKCtheta, a T cell-specific kinase important for CBM assembly and classical NF-kappaB activation. We further demonstrate in vitro NOTCH1 associates physically with PKCtheta and CARMA1 in the cytosol. Unexpectedly, when NOTCH1 expression was abrogated using RNAi approaches, interactions between CARMA1, BCL10 and MALT1 were lost. This failure in CBM assembly reduced IkappaBalpha phosphorylation and diminished NF-kappaB-DNA binding. Finally, using a luciferase gene reporter assay, we show the intracellular domain of NOTCH1 can initiate robust NF-kappaB activity in stimulated T cells, even when NOTCH1 is excluded from the nucleus through modifications that restrict it to the cytoplasm or hold it tethered to the membrane. Collectively, these observations provide evidence that NOTCH1 may facilitate early events during T cell activation by nucleating the CBM complex and initiating NF-kappaB signaling.

  20. NF-Kβ Activation in U266 Cells on Mesenchymal Stem Cells

    Science.gov (United States)

    Zahedi, Sara; Shamsasenjan, Karim; Movassaghpour, Aliakbar; Akbarzadehlaleh, Parvin

    2016-01-01

    Purpose: Mesenchymal Stem Cells (MSCs) are one of the essential members of Bone Marrow (BM) microenvironment and the cells affect normal and malignant cells in BM milieu. One of the most important hematological malignancies is Multiple Myeloma (MM). Numerous studies reported various effects of MSCs on myeloma cells. MSCs initiate various signaling pathways in myeloma cells, particularly NF-kβ. NF-kβ signaling pathway plays pivotal role in the survival, proliferation and resistance of myeloma cells to the anticancer drugs, therefore this pathway can be said to be a vital target for cancer therapy. This study examined the relationship between U266 cells and MSCs. Methods: U266 cells were cultured with Umbilical Cord Blood derived-MSCs (UCB-MSCs) and Conditioned Medium (C.M). Effect of UCB-MSCs and C.M on proliferation rate and CD54 expression of U266 cells were examined with MTT assay and Flowcytometry respectively. Furthermore, expression of CXCL1, PECAM-1, JUNB, CCL2, CD44, CCL4, IL-6, and IL-8 were analyzed by Real Time-PCR (RT-PCR). Moreover, status of p65 protein in NF-kβ pathway assessed by western blotting. Results: Our findings confirm that UCB-MSCs support U266 cells proliferation and they increase CD54 expression. In addition, we demonstrate that UCB-MSCs alter the expression of CCL4, IL-6, IL-8, CXCL1 and the levels of phosphorylated p65 in U266 cells. Conclusion: Our study provides a novel sight to the role of MSCs in the activation of NF-kβ signaling pathway. So, NF-kβ signaling pathway will be targeted in future therapies against MM.

  1. Induction of nuclear translocation of NF-kappaB in epithelial cells by respirable mineral fibres.

    Science.gov (United States)

    Brown, D M; Beswick, P H; Donaldson, K

    1999-10-01

    A panel of mineral fibres has been studied for their ability to cause translocation of the transcription factor NF-kappaB to the nucleus in A549 lung epithelial cells. On the basis of inhalation studies, three fibres were designated as being carcinogenic-amosite asbestos, silicon carbide and refractory ceramic fibre 1 (RCF1)-or non-carcinogenic-man-made vitreous fibre (MMVF10), Code 100/475 glass fibre, and RCF4. The experiments were carried out at equal fibre number. It was hypothesized that carcinogenic fibres have greater free radical activity than non-carcinogenic fibres and that an oxidative stress produced in the lung after inhalation of fibres could cause translocation of the transcription factor NF-kappaB to the nucleus, where transcription of pro-inflammatory genes such as cytokines could occur. It was demonstrated that a simple oxidant, hydrogen peroxide, caused translocation in a time- and dose-dependent manner. The three carcinogenic fibres produced a significant dose-dependent translocation of NF-kappaB to the nucleus, whereas the non-carcinogenic fibres did not. Silicon carbide fibres were the most potent of the pathogenic fibres. MMVF10 was the most potent of the non-pathogenic fibres, causing significant nuclear translocation of NF-kappaB at high fibre number. Using three antioxidants, curcumin, pyrrolidine dithiocarbamate, and Nacystelin, translocation caused by carcinogenic fibres could be significantly reduced. The present study shows that a short-term in vitro assay can discriminate between pathogenic and non-pathogenic fibres in terms of a key pro-inflammatory event in epithelial cells. The mechanism of the activation of NF-kappaB by pathogenic fibres and its general applicability to other fibre types remain to be determined.

  2. ISO sertifikaat kuulutab tootja mainet üle maailma / Ain Alvela

    Index Scriptorium Estoniae

    Alvela, Ain, 1967-

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 12. apr. lk. 24-25. ISO standardi rakendamine tagab toodangu kvaliteedi ja annab firmale usaldusväärsuse. Kommenteerib Ain Noormägi. Vt. samas: ISO 9001; ISO 14 001; Universaalne standard kohandub ettevõtte vajadustega

  3. Antioxidant mechanisms of iso-6-cassine in suppressing seizures induced by pilocarpine

    Directory of Open Access Journals (Sweden)

    Rivelilson Mendes de Freitas

    2011-06-01

    Full Text Available The aim of this study was to evaluate the in vitro antioxidant effects of 12-[(2R,5R,6R-5-hydroxy-6-methylpiperidin-2-yl]dodecan-2-one (iso-6-cassine; ISO and the anticonvulsant effects of ISO on pilocarpine-induced seizures in rats. Wistar rats were treated with 0.9% saline (i.p., control group, pilocarpine (400 mg/kg, i.p., pilocarpine group, and the association of ISO (1.0 mg/kg, i.p. plus pilocarpine (400 mg/kg, i.p., 30 min after administration of ISO (ISO plus pilocarpine group. After the treatments all groups were observed for 1h. The antioxidant effect of ISO on the pilocarpine model was assessed by determining the activity of glutathione peroxidase (GPx, glutathione-S-transferase (GST and catalase (CAT as well as the levels of reactive species (RS and lipid peroxidation (LP. In vitro, ISO (5 μM reduced RS and LP. ISO (1.0 mg/kg and abolished seizures and death induced by pilocarpine in rats. ISO protected against the increase in the RS and LP levels, GST activity as well as the inhibition of GPx activity caused by pilocarpine. In addition, ISO increased the catalase activity in hippocampus of seized rats. In conclusion, the dta suggest that ISO can present anticonvulsant and antioxidant properties in the pilocarpine model of seizures in rats.

  4. Juhtimissüsteemide selgroog. Lean ning ISO - terviklahendused tootmise haldamiseks / Anton Ovsjankin

    Index Scriptorium Estoniae

    Ovsjankin, Anton

    2011-01-01

    Kasvanud on juurutatavate Lean ja ISO süsteemide arv, kuid on kasvanud ka nende juurutamise ja arendamisega seotud ebaõnnestumiste arv. Põhjustest, miks jäävad paljud Leani ja ISO juurutanud ettevõtted ilma lubatud tulemusteta. Leani ja ISO haldamiseks vajalikest teguritest

  5. 75 FR 3228 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2010-01-20

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings January 12... Commission staff may attend the following Midwest ISO-related meetings: Advisory Committee (10 a.m.-3 p.m... October 20 November 17 December 1 Midwest ISO Informational Forum (3 p.m.-5 p.m., ET) January 19...

  6. 76 FR 4104 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2011-01-24

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings January 13... Commission staff may attend the following Midwest ISO-related meetings during the 2011 year: Advisory.... Paul, MN) September 14 October 19 November 16 December 6 Midwest ISO Informational Forum (3 p.m.-5...

  7. 78 FR 38027 - ISO New England Inc.; Notice of Initiation of Proceeding and Refund Effective Date

    Science.gov (United States)

    2013-06-25

    ... Energy Regulatory Commission ISO New England Inc.; Notice of Initiation of Proceeding and Refund... determine the justness and reasonableness of section III.A.15 of Appendix A of ISO New England Inc.'s existing tariff. Dominion Energy Marketing, Inc. and ISO New England Inc., 143 FERC ] 61,233 (2013)....

  8. 75 FR 5779 - Notice Providing Agenda for Technical Conference on RTO/ISO Responsiveness

    Science.gov (United States)

    2010-02-04

    ... Energy Regulatory Commission Notice Providing Agenda for Technical Conference on RTO/ISO Responsiveness.... ER09-1050-000, ER09- 1192-000. ISO New England, Inc. and New England Docket No. ER09-1051-000. Power... , (202) 502-8048. Kimberly D. Bose, Secretary. Agenda--Technical Conference on RTO/ISO...

  9. 75 FR 16096 - New England Power Generators Association Inc., Complainant v. ISO New England Inc., Respondent...

    Science.gov (United States)

    2010-03-31

    ... Energy Regulatory Commission New England Power Generators Association Inc., Complainant v. ISO New England Inc., Respondent; ISO New England Inc. and New England Power Pool; Notice of Complaint March 24... Generators Association Inc. (Complainant) filed a formal complaint against ISO New England Inc....

  10. Uncertainty budget for final assay of a pharmaceutical product based on RP-HPLC

    DEFF Research Database (Denmark)

    Heydorn, Kaj; Anglov, Thomas; Byrialsen, Kirsten

    2003-01-01

    Compliance with specified limits for the content of active substance in a pharmaceutical drug requires knowledge of the uncertainty of the final assay. The uncertainty of measurement is based on the ISO recommendation as expressed in the Guide to the Expression of Uncertainty in Measurement (GUM...

  11. Pyrrolidine dithiocarbamate Inhibits NF- κB Activation and Enhance TNFα- Induced Apoptosis in Human Breast Cancer Cells

    Institute of Scientific and Technical Information of China (English)

    TU Gang(涂刚); YAO Zhenxiang(姚榛祥); DONG Pujiang(董浦江)

    2002-01-01

    Objective: Tumor necrosis factor α(TNFα) induced apoptosis is limited by its coactivation of nuclear factor kappa B(NF- κB) -dependent antiapoptosis genes. We examined whether pyrrolidine dithiocarbamate (PDTC) enhance TNFα - induced apoptosis in cultured breast cancer cells and explored the role of NF - κB in TNFα - induced apoptosis. Methods: Human breast cancer cell lines MCF-7 and MDA - MB -435s were treated with TNFα、 PDTC and combination therapy . Induction of apoptosis was detected by TUNEL staining and flow cytometry. NF- κB DNA binding activity was detected using electrophoresis mobility shift assay(EMSA) . Western blots of cytoplasmic lysates were performed to demonstrate IκBα (Inhibitor protein of nuclear factor κB) phosphorylation and degradation. Results:TNFα-induced IκBo phosphorylation and degradation, which was inhibited by PDTC in both cell lines. TNFα-induced apoptosis (TUNEL) increased significantly when both cells were pretreated with PDTC. Flow cytometry also confirmed this. EMSA showed that PDTC continuously inhibited TNFo-induced NF- κB DNA binding activity . Conclusions:PDTC enhances TNFo-induced apoptosis whileinhibiting IκBα phosphorylation and degradation in human breast cancer cells. NF - κB has a protective role on TNFα-induced apoptosis.

  12. Effects of digoxin on cell cycle, apoptosis and NF-κB pathway in Burkitt's lymphoma cells and animal model.

    Science.gov (United States)

    Wang, Ting; Xu, Peipei; Wang, Fan; Zhou, Di; Wang, Ruju; Meng, Li; Wang, Xiaohui; Zhou, Min; Chen, Bing; Ouyang, Jian

    2017-01-13

    Digoxin has potential antitumor properties. This study investigated whether digoxin suppressed Burkitt's lymphoma (BL) cells. Raji and NAMALWA cells were exposed to digoxin, followed by assay of cell viability, apoptosis and cell cycle. Western blotting was used to analyze NF-κB activity. A xenograft model was established for therapeutic efficacy evaluation. Digoxin inhibited cell growth and resulted in apoptosis and cell cycle arrest (G0/G1 for Raji cells; G2/M for NAMALWA cells). Digoxin inhibited DNA synthesis and induced morphological apoptotic characteristics. Besides, digoxin inhibited NF-κB and TNF-α-stimulated NF-κB activity, and suppressed NF-κB initiating genes (Bcl-2, Bcl-xL, cyclin D1, and c-myc), however, increased p21(cip1). Digoxin activated caspase-9/3. Furthermore, digoxin inhibited xenograft tumors growth and reduced Ki-67 and c-myc. Digoxin exerted antitumor effects on BL cells in vitro and in vivo might through regulating NF-κB and caspase pathway. These outcomes highlight the potential of digoxin as a therapeutic agent for BL.

  13. TGEV infection up-regulates FcRn expression via activation of NF-κB signaling.

    Science.gov (United States)

    Guo, Jinyue; Li, Fei; Qian, Shaoju; Bi, Dingren; He, Qigai; Jin, Hui; Luo, Rui; Li, Shaowen; Meng, Xianrong; Li, Zili

    2016-08-24

    It has been well characterized that the neonatal Fc receptor (FcRn) transports maternal IgG to a fetus or newborn and protects IgG from degradation. We previously reported that FcRn is expressed in a model of normal porcine intestinal epithelial cells (IPEC-J2). Transmissible gastroenteritis is an acute enteric disease of swine that is caused by transmissible gastroenteritis virus (TGEV). How porcine FcRn (pFcRn) expression is regulated by pathogenic infection remains unknown. Our research shows that IPEC-J2 cells infected with TGEV had up-regulated pFcRn expression. In addition, the NF-κB signaling pathway was activated in IPEC-J2 cells by TGEV infection. Furthermore, treatment of TGEV-infected IPEC-J2 cells with the NF-κB-specific inhibitor BAY 11-7082 resulted in down-regulation of pFcRn expression. Transient transfection of pFcRn promoter luciferase report plasmids with overexpression of NF-κB p65 transcription factor enhanced the activation of the luciferase report plasmids. We identified four NF-κB transcription factor binding sites in the promoter region of this gene using luciferase reporter system, chromatin immunoprecipitation, electromobility shift assay, and supershift analysis. Together, the data provide the first evidence that TGEV infection up-regulates pFcRn expression via activation of NF-κB signaling.

  14. 企業経営とISO14000

    OpenAIRE

    佐々木,浩幸

    1999-01-01

    今日の日本の企業のほとんどは,国際化の中での経営活動を展開している。このため,国際標準化機構(ISO)の定めた国際規格を取得することが,国際的な経営活動をする企業として生き残るために不可欠な要素となった。今日ISOのうちでも注目されているのは, 9000と14000である。ISO9000は品質システムに関する国際規格で,ISO14000は,環境マネジメントシステムに関する国際規格である。本論文では,全人類の課題である環境問題の重要性の視点から,このISO14000と企業経営について論じた。まず, ISO14000取得のメリットデメリットについて論じ,世界及び日本におけるISO14000の取得状況について述べた。特に日本の状況については,都道府県別にその特徴の分析を,基本的データを分かりやすく加工して,行った。そして,日本の企業の将来のためには,ISO14000取得は必須条件であり,取得のための問題点,その解決策,将来展望について論じた。...

  15. ISO/FDIS 9001:2008 and Its Implementation%2008版ISO9001标准及其实施建议

    Institute of Scientific and Technical Information of China (English)

    田武

    2008-01-01

    文章介绍了2008版ISO/FDIS 9001标准的起草情况以及相对于2000版标准的主要变化,根据2008年5月第25届ISO/TC 176大会的决议,提出了我国企业实施新版ISO 9001标准的对策建议.

  16. 从管理学的视角解读ISO 9001:2008%Interpretation of ISO 9001:2008 from the View of Management

    Institute of Scientific and Technical Information of China (English)

    韦斌生

    2009-01-01

    @@ ISO 9001:2008新版标准终于在2008年11月15日正式发布.根据ISO(国际标准化组织)官方发布的公告,基于ISO 9001:2000标准发布8年来,全球170个国家一百多万个组织认证的经验.

  17. The NF1 gene contains hotspots for L1 endonuclease-dependent de novo insertion.

    Directory of Open Access Journals (Sweden)

    Katharina Wimmer

    2011-11-01

    Full Text Available Long interspersed (L1 and Alu elements are actively amplified in the human genome through retrotransposition of their RNA intermediates by the -100 still retrotranspositionally fully competent L1 elements. Retrotransposition can cause inherited disease if such an element is inserted near or within a functional gene. Using direct cDNA sequencing as the primary assay for comprehensive NF1 mutation analysis, we uncovered in 18 unrelated index patients splicing alterations not readily explained at the genomic level by an underlying point-mutation or deletion. Improved PCR protocols avoiding allelic drop-out of the mutant alleles uncovered insertions of fourteen Alu elements, three L1 elements, and one poly(T stretch to cause these splicing defects. Taken together, the 18 pathogenic L1 endonuclease-mediated de novo insertions represent the largest number of this type of mutations characterized in a single human gene. Our findings show that retrotransposon insertions account for as many as -0.4% of all NF1 mutations. Since altered splicing was the main effect of the inserted elements, the current finding was facilitated by the use of RNA-based mutation analysis protocols, resulting in improved detection compared to gDNA-based approaches. Six different insertions clustered in a relatively small 1.5-kb region (NF1 exons 21(16-23(18 within the 280-kb NF1 gene. Furthermore, three different specific integration sites, one of them located in this cluster region, were each used twice, i.e. NM_000267.3(NF1:c.1642-1_1642 in intron 14(10c, NM_000267.3(NF1:c.2835_2836 in exon 21(16, and NM_000267.3(NF1:c.4319_4320 in exon 33(25. Identification of three loci that each served twice as integration site for independent retrotransposition events as well as 1.5-kb cluster region harboring six independent insertions supports the notion of non-random insertion of retrotransposons in the human genome. Currently, little is known about which features make sites

  18. The PRR11-SKA2 Bidirectional Transcription Unit Is Negatively Regulated by p53 through NF-Y in Lung Cancer Cells.

    Science.gov (United States)

    Wang, Yitao; Weng, Huali; Zhang, Ying; Long, Yinjiang; Li, Yi; Niu, Yulong; Song, Fangzhou; Bu, Youquan

    2017-03-01

    We previously identified proline-rich protein 11 (PRR11) as a novel cancer-related gene that is implicated in the regulation of cell cycle and tumorigenesis. Our recent study demonstrated that PRR11 and its adjacent gene, kinetochore associated 2 (SKA2), constitute a classic head-to-head gene pair that is coordinately regulated by nuclear factor Y (NF-Y). In the present study, we further show that the PRR11-SKA2 bidirectional transcription unit is an indirect target of the tumor suppressor p53. A luciferase reporter assay revealed that overexpression of wild type p53, but not mutant p53, significantly represses the basal activity and NF-Y mediated transactivation of the PRR11-SKA2 bidirectional promoter. Deletion and mutation analysis of the PRR11-SKA2 promoter revealed that p53-mediated PRR11-SKA2 repression is dependent on the presence of functional NF-Y binding sites. Furthermore, a co-immunoprecipitation assay revealed that p53 associates with NF-Y in lung cancer cells, and a chromatin immunoprecipitation assay showed that p53 represses PRR11-SKA2 transcription by reducing the binding amount of NF-Y in the PRR11-SKA2 promoter region. Consistently, the ability of p53 to downregulate PRR11-SKA2 transcription was significantly attenuated upon siRNA-mediated depletion of nuclear factor Y subunit beta (NF-YB). Notably, lung cancer patients with lower expression of either PRR11 or SKA2 along with wild type p53 exhibited the best overall survival compared with others with p53 mutation and/or higher expression of either PRR11 or SKA2. Taken together, our results demonstrate that p53 negatively regulates the expression of the PRR11-SKA2 bidirectional transcription unit through NF-Y, suggesting that the inability to repress the PRR11-SKA2 bidirectional transcription unit after loss of p53 might contribute to tumorigenesis.

  19. PYRROLIDINE DITHIOCARBAMATE INHIBITS NF-ΚB ACTIVATION AND ENHANCES TNF-INDUCED APOPTOSIS IN HUMAN BREAST CANCER CELLS

    Institute of Scientific and Technical Information of China (English)

    涂刚; 姚榛祥

    2004-01-01

    Objective: To determine whether pyrrolidine dithio- carbamate(PDTC) enhances TNFκ-induced apoptosis in cultured breast cancer cells and explore the role of NF-κB in TNFα-induced apoptosis. Methods: Human breast cancer cell lines MCF-7 and MDA-MB-435s were treated with TNFα, PDTC and combination therapy. Cell survivals were determined by MTT assay. Apoptosis was detected by TUNEL and flow cytometry. NF-κ B DNA binding activity was detected using electrophoresis mobility shift assay (EMSA). Western blots were performed to demonstrate IκBα(Inhibitor protein of nuclear factor(B) phosphorylation and degradation. Results: Cell growth was not suppressed by either TNFα(2000 U/ml or less) or PDTC alone. Both cell lines treated with TNFα(2000 U/ml) combined with PDTC(50 μmol/L) showed significant growth inhibition. PDTC inhibited TNFα-induced IκBα phosphorylation and degradation in both cell lines. EMSA showed that PDTC continuously inhibited TNFα induced NF-κB DNA binding activity. TNFα induced apoptosis (TUNEL) was increased significantly when both cells were pretreated with PDTC, and this was confirmed by Flow cytometry. Conclusion: PDTC enhances TNFα-induced apoptosis via inhibiting IκBα phosphorylation and degradation in human breast cancer cells. NF-κB protects against TNFα-induced apoptosis.

  20. Prediction of Time, Cost and Effort needed for software organizations to transit from ISO 9001:2008 to ISO 9001:2015. : A Survey

    OpenAIRE

    Somaraju, Dilip

    2016-01-01

    Context. Several quality standards have been developed over the years in order to define quality metrics for an organization’s product and even processes. One of the famous standards among them is the ISO 9000 standards which started several years ago. Since its beginning, ISO standards have seen several upgrades. Currently ISO 9001:2008 is in use which is being upgraded to ISO 9001: 2015. Companies have to migrate to the new scheme within three years of the prescribed time in order to retain...

  1. ISO 9000 Family Standards in China——Interview with Wang Zhongmin, President of CNIS, at ISO/TC 176 Meeting

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    China Standardization:Could you please talk about the promotion and development of ISO 9000 family standards in China?Wang Zhongmin:China began the implementation of national standards equivalent to ISO 9000-1987 on August 1,1989.On December 2,1989,CSBTS/TC 151 was established responsible for the development,revision,promotion and implementation of national standards concerning quality management and quality assurance,coordinating with ISO/TC 176.China started the implementation of equivalent national standards in 1992,converted ISO 9000 series standards respectively in 1994,2000 and 2008,and made great efforts in promotion and implementation of those standards.

  2. New Thinking on Quality Management Standards——Interview with Dr.Gary Cort, the Chair of ISO/TC 176

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    China Standardization:We know that ISO/TC 176 is one of the most important and influential technical committees of ISO,ISO 9000 family standards developed by ISO/TC 176 are well-known worldwide and set a successful example for international standards promotion and application,would you please tell us about the Scope of ISO/TC 176,and the global impact of ISO 9000 family standards developed by ISO/TC 176.Gary Cort:ISO/TC 176 is responsible for a suite of quality managemeng system standards including ISO 9000,ISO 9001,and ISO 9004.

  3. Vocational High School Effectiveness Standard ISO 9001: 2008 for Achievement Content Standards, Standard Process and Competency Standards Graduates

    Directory of Open Access Journals (Sweden)

    Yeni Ratih Pratiwi

    2014-06-01

    Full Text Available Efektivitas Sekolah Menengah Kejuruan Berstandar ISO 9001:2008 terhadap Pencapaian Standar Isi, Standar Proses dan Standar Kompetensi Lulusan Abstract: The purpose of this study was to determine differences in the effectiveness of the achievement of the content standards, process standards, and competency standards in vocational already standard ISO 9001: 2008 with CMS that has not been standardized ISO 9001: 2008 both in public schools and private schools. Data collection using the questionnaire enclosed Likert scale models. Analysis of data using one-way ANOVA using SPSS. The results showed: (1 there is no difference in effectiveness between public SMK ISO standard ISO standards with private SMK (P = 0.001; (2 there are differences in the effectiveness of public SMK SMK ISO standards with ISO standards have not (P = 0.000; (3 there are differences in the effectiveness of public SMK ISO standards with private vocational yet ISO standards (P = 0.000; (4 there are differences in the effectiveness of the private vocational school with vocational standard ISO standard ISO country has not (P = 0.015; (5 there are differences in the effectiveness of the private vocational bertandar ISO with private vocational yet standardized ISO (P = 0.000; (6 there was no difference in the effectiveness of public SMK has not been standardized by the ISO standard ISO private SMK yet. Key Words: vocational high school standards ISO 9001: 2008, the standard content, process standards, competency standards Abstrak: Tujuan penelitian ini untuk mengetahui perbedaan efektivitas pencapaian standar isi, standar proses, dan standar kompetensi lulusan pada SMK yang sudah berstandar ISO 9001:2008 dengan SMK yang belum berstandar ISO 9001:2008 baik pada sekolah negeri maupun sekolah swasta. Pengumpulan data menggunakan kuisioner tertutup model skala likert. Analisis data menggunakan ANOVA one way menggunakan program SPSS. Hasil penelitian menunjukkan: (1 ada perbedaan

  4. Oncogene Mutation Survey in MPNST Cell Lines Enhances the Dominant Role of Hyperactive Ras in NF1 Associated Pro-Survival and Malignancy.

    Science.gov (United States)

    Sun, Daochun; Tainsky, Michael A; Haddad, Ramsi

    2012-01-01

    Malignant peripheral nerve sheath tumors (MPNST) are a type of soft tissue sarcoma that can be associated with germline mutations in Neurofibromatosis type 1 (NF1) or may occur sporadically. Although the etiology of MPNST is poorly understood, it is clear that a loss of function of the NF1 gene, encoding a Ras-GAP, is an important factor in the tumorigenesis of the inherited form of MPNST. Tumor latency in NF1 patients suggests that additional mutational events are probably required for malignancy. In order to define oncogene mutations associated with 5 MPNST cell lines, we assayed the 238 most frequent mutations in 19 commonly activated oncogenes using mass spectroscopy-based analysis. All 238 mutation sites in the assayed oncogenes were determined to harbor only wild-type sequences. These data suggest that hyperactive Ras resulting from the loss function of neurofibromin may be sufficient to set up the direction of malignant transformation of Schwann cells to MPNST.

  5. Why did ISO 9001:2008 system fail to deliver?

    Science.gov (United States)

    Langford, Melvyn

    2014-02-01

    This article is based on an actual investigation undertaken, and summarises the subsequent report's findings and observations. It has been anonymised for obvious reasons. In May 2013 an analysis was undertaken by a multidisciplinary team that compared an NHS Trust estates department's managerial systems against the NHS national recommendations. The conclusions stated that: 'There was a systemic failure across a large number of topics generating intolerable/substantial risks to the organisation, its staff, and patients; this despite the department's managerial systems being accredited to the International Standard ISO 9001:2008'. The natural question raised when presented with this demonstrable and auditable evidence was: 'Why did the ISO 9001:2008 system fail?'

  6. Analysis to Maturity Levels in CMMI and ISO Models

    Directory of Open Access Journals (Sweden)

    Linda Macalister

    2012-12-01

    Full Text Available Both CMMI as ISO are important models with widely acceptance for evaluating and improving software processes. Both structure and carry out common continuous representations but as models are different and proposed various sets of processes differentiated primarily on the structure of its stages. CMMI is based on the classic idea of the 5 maturity levels proposed by Humphrey in 1988, while ISO promotes acontinuous model to evaluate the process capability. However, this community has recognized the benefits of a staged representation and the standard 2008 version introduced the framework of organizational maturity of 6 levels. This paper investigates and analyzes the relationship between levels of maturity and process capability ofthese two models.

  7. ISO(4,1) symmetry in the EFT of inflation

    Science.gov (United States)

    Creminelli, Paolo; Emami, Razieh; Simonović, Marko; Trevisan, Gabriele

    2013-07-01

    In DBI inflation the cubic action is a particular linear combination of the two, otherwise independent, cubic operators dot pi3 and dot pi(∂iπ)2. We show that in the Effective Field Theory (EFT) of inflation this is a consequence of an approximate 5D Poincar&apose symmetry, ISO(4,1), non-linearly realized by the Goldstone π. This symmetry uniquely fixes, at lowest order in derivatives, all correlation functions in terms of the speed of sound cs. In the limit cs → 1, the ISO(4,1) symmetry reduces to the Galilean symmetry acting on π. On the other hand, we point out that the non-linear realization of SO(4,2), the isometry group of 5D AdS space, does not fix the cubic action in terms of cs.

  8. ISO(4,1) Symmetry in the EFT of Inflation

    CERN Document Server

    Creminelli, Paolo; Simonović, Marko; Trevisan, Gabriele

    2013-01-01

    In DBI inflation the cubic action is a particular linear combination of the two, otherwise independent, cubic operators \\dot \\pi^3 and \\dot \\pi (\\partial_i \\pi)^2. We show that in the Effective Field Theory (EFT) of inflation this is a consequence of an approximate 5D Poincar\\'e symmetry, ISO(4,1), non-linearly realized by the Goldstone \\pi. This symmetry uniquely fixes, at lowest order in derivatives, all correlation functions in terms of the speed of sound c_s. In the limit c_s \\to 1, the ISO(4,1) symmetry reduces to the Galilean symmetry acting on \\pi. On the other hand, we point out that the non-linear realization of SO(4,2), the isometry group of 5D AdS space, does not fix the cubic action in terms of c_s.

  9. [Quality assurance in oncology: experiences of an ISO certification].

    Science.gov (United States)

    Szentirmay, Zoltán; Cseh, Lujza; Ottó, Szabolcs; Kásler, Miklós

    2002-01-01

    The ISO 9001 quality assurance of the National Institute of Oncology has been achieved successfully. We give an account of the brief history and the structure of the assurance system of the Institute, the process of setting our goals, and also the experience gained from drafting ISO 9001 handbook and flowcharts. Apart from the bureaucratic nature of quality assurance, it is a good opportunity for us to investigate our everyday work, put it into orderly manner and work more reliably. Experience has shown that the introduction of a quality assurance system increases the level of patient care, the documentation helps the Institute or some of its departments, or even individuals prevent law suits, and serves as a sound basis for proposing promotion, salary increases and bonuses, or even honors.

  10. Composite repairs qualification according to ISO/TS 24817

    Energy Technology Data Exchange (ETDEWEB)

    Meniconi, Luiz C.M.; Perrut, Valber A. [Petroleo Brasileiro S.A. (PETROBRAS/CENPES), Rio de Janeiro, RJ (Brazil). Centro de Pesquisas

    2009-07-01

    Composite repairs for metallic pipes from three different suppliers were evaluated according to ISO Technical Specification TS 24817. The intended application scenarios are offshore production plants, but the design methodology is also applicable to onshore pipelines and pipework. The evaluation covered all the relevant mechanical properties and established the maximum application temperatures for each repair system. The tests also considered the application of composite repair sleeves to metallic pipes with through thickness defects, by measuring the strain energy release rates of the composite-metal interfaces. The test campaigns aimed to verify the applicability of the recently published ISO document, and to implement it as the routine procedure for composite repairs evaluation within PETROBRAS. The tests also addressed the influence of metallic pipe surface preparation on the final properties of the repair sleeves, especially in relation to the long term behavior of leaking pipes repaired by means of composite materials. (author)

  11. Quality control with R an ISO standards approach

    CERN Document Server

    Cano, Emilio L; Prieto Corcoba, Mariano

    2015-01-01

    Presenting a practitioner's guide to capabilities and best practices of quality control systems using the R programming language, this volume emphasizes accessibility and ease-of-use through detailed explanations of R code as well as standard statistical methodologies. In the interest of reaching the widest possible audience of quality-control professionals and statisticians, examples throughout are structured to simplify complex equations and data structures, and to demonstrate their applications to quality control processes, such as ISO standards. The volume balances its treatment of key aspects of quality control, statistics, and programming in R, making the text accessible to beginners and expert quality control professionals alike. Several appendices serve as useful references for ISO standards and common tasks performed while applying quality control with R.

  12. ENVIRONMENTAL RESPONSIBILITY MODEL BASED ON ISO 14000 MANAGEMENT SYSTEMS

    Directory of Open Access Journals (Sweden)

    Catalina SITNIKOV

    2012-01-01

    Full Text Available Worldwide corporations, as well as their stakeholders, are more conscious of the need for environmental management, SR behaviour, and sustainable growth and development. International Standards are becoming more significant for corporations to work towards common environmental management practices. ISO 14001 is the first and the broadest standard intended at a more responsible approach of corporations and the world’s most acknowledged framework for environmental management systems that assists corporations to better manage the effect of their activities on the environment. This article aims to study ISO 14001 implementation and its effects on the environmental responsibility. A model will be built, which covers the environmental management system, the components of organizational culture, being able to influence environmental standards implementation.

  13. A Cross-Country Empirical Study Towards the Impact of Following ISO Management System Standards on Euro-Area Economic Confidence

    OpenAIRE

    Ştefan Daniel Armeanu; Georgeta Vintilă; Ştefan Cristian Gherghina

    2017-01-01

    This study aims to examine the impact of following ISO management system standards on economic sentiment indicator (ESI) as proxy of economic agents’ general view concerning economic activity, for 21 European Union member states over 2005-2014. The empirical research comprises ISO standards with reference to management systems towards quality (ISO 9001, ISO 13485, ISO 16949), food safety (ISO 22000), environment (ISO 14001), and information security (ISO 27001). Panel data fixed effects regre...

  14. ISO9001:2008标准的用户调查介绍

    Institute of Scientific and Technical Information of China (English)

    田武

    2010-01-01

    @@ ISO 9001:2008 已于2008年11月15日正式发布,承担该标准修订工作的TC 176/SC 2质量体系分委员会WG 18/TG 1.19解散.在2009年ISO/TC 176会议上,成立了新的工作组,即ISO 9001标准未来修订工作组(Future revision to ISO 9001),探讨ISO 9001:2008标准的未来修订事宜.

  15. [Recommendations for implementing the UNE-EN-ISO 15189 quality standard in the clinical microbiology laboratory: bacteriology and serology].

    Science.gov (United States)

    Rojo, María Dolores; Aguiar, Juan Manuel; Cercenado, Emilia; de Ory, Fernando; de la Rosa, Manuel

    2010-11-01

    The UNE-EN-ISO 15189:2007 standard specifies the management and technical requirements that clinical microbiology laboratories must meet to achieve optimal quality when performing microbiological analyses. With implementation of this standard, a laboratory can receive the accreditation and formal recognition of an authorized body, certifying that it is apt for performing an assay or group of assays. In Spain, laboratories that apply these standards can be accredited by the Entidad Nacional de Acreditación (ENAC, Spanish accreditation body). The purpose of this review is to familiarize clinical microbiology laboratory specialists with the UNE-EN-ISO 15189:2007 standard through a practical approach focussed on bacteriology and serology studies. We briefly define the scope and specify the requisites required for managing the quality of the procedures and processes involved in performing tests on human specimens, for document control, and for management of instruments and equipment, personnel, information systems, supply systems, and external services. Lastly, evaluation approaches are indicated to achieve continuing improvement of the processes carried out and the services the laboratory provides.

  16. Gap Analysis Comparing LLNL ISMS and ISO 14001

    Energy Technology Data Exchange (ETDEWEB)

    Doerr, T B

    2004-08-09

    A gap analysis was conducted comparing the Lawrence Livermore National Laboratory (LLNL) Integrated Safety Management System (ISMS) with the international standard ISO 14001 Environmental Management System and with Department of Energy (DOE) Order 450.1. This analysis was accomplished as part of LLNL's assessment of the impacts of adopting DOE Order 450.1 and comprises a portion of its continuous improvement efforts under ISMS.

  17. ISOS: A job evaluation system to implement comparable worth

    Directory of Open Access Journals (Sweden)

    Albert Corominas

    2008-01-01

    Full Text Available A fair pay structure is an essential element of the personnel policy of a firm. If the pay structure is perceived as arbitrary by the members of the staff, it becomes a cause of disturbance of the labor relations. Particularly, a pay structure is unfair if it discriminates against women. Job evaluation is a traditional tool used by companies to assist in the process of determining pay structures that can be also useful to detect and combat wage discrimination, since allow determining whether two jobs are of comparable worth or not. Although there are many kinds of systems, authors agree when defining point factor methods as the most appropriate and fair job evaluation systems. However, even being well defined from a technical point of view, most existing systems give discriminatory results regarding to gender. ISOS, a new job evaluation system which is described in this paper, has been designed, with the aim to define a neutral system with regard to gender, based on present jobs characteristics, existing job evaluation systems and job description questionnaires, international experts’ knowledge and a wide body of literature on gender discrimination and its relation with job evaluation. Using ISOS can contribute to detect, combat and eliminate part of the existing wage discrimination in general and, in particular, against women. ISOS includes all aspects of the work so no characteristics are omitted. The system can be applied in any company and to evaluate any job, and offers flexibility to be adapted to the specific characteristics of an organization. ISOS can also be used to detect and combat wage discrimination. Furthermore, characteristics of present jobs, such as cross-training or flexible working time, are also included so the system can be considered innovative in a very traditional field of industrial engineering.

  18. ISO 14000 Y LA GESTIÓN MEDIO AMBIENTAL

    OpenAIRE

    2014-01-01

    ISO 14000 es un conjunto de Normas Internacionales de carácter voluntario, orientadas hacia la gestión efectiva del medio ambiente en las organizaciones. Implica el desarrollo de requisitos concretos de los Sistemas de Gestión Ambiental, la Auditoría Ambiental, el Eco­ Etiquetado y el ciclo de vida de Productos, Servicios y Procesos dentro de las aspiraciones de las Organizaciones.

  19. NF1 Signal Transduction and Vascular Dysfunction

    Science.gov (United States)

    2015-05-01

    as well as clinically available pharmaceuticals , we are determining the critical regulatory pathways that drive the changes seen following the loss of...loss in the adult endothelium and can serve as a model system for investigation of both cardiovascular effects and the tumor microenvironment. Body: Aim...Korf, B. R. (2002). Cardiovascular disease in neurofibromatosis 1: report of the NF1 Cardiovascular Task Force. Genet Med 4, 105-111. Munchhof, A. M

  20. ISO26000:国外已先行一步

    Institute of Scientific and Technical Information of China (English)

    王琛; 于帆; 郝琴

    2011-01-01

    2011年,ISO 26000社会责任国际标准实施应用已经历时一年,其影响辐射到世界每一个区域.为了做好ISO 26000的推广实施以及不同语种的翻译出版工作,近期,ISO 26000"后出版组织"(Post Publication Organization,以下简称"PPO")开展了有关该标准在各国实施应用情况的调查.具体调查情况如下.调查范围2011年10月,PPO在全体ISO成员国范围内开展社会责任标准实施应用调查,收到65个成员国的有效反馈意见,占ISO成员国总数的40.1%,具体国家列表见表1.

  1. Does implementation of ISO standards in hospitals improve patient satisfaction?

    Directory of Open Access Journals (Sweden)

    Vahid Keshtkar

    2017-01-01

    Full Text Available Introduction: Around the world, a large number of projects have been developed with the aim of assessing patient satisfaction especially in hospitals. As an important indicator of the quality of health care system, Patients’ perception of health care has been the center of attention over the recent 20 years. Method: 402 patients who were hospitalized in teaching hospitals affiliated to the Shiraz University of Medical Sciences were investigated. Patients’ satisfactions of the health care services were assessed using the translated and modified version of the KQCAH consisted of 44 questions divided to7 categories of Respect and Caring, Effectiveness and Continuity, Appropriateness, Information, Efficiency, Meals, First Impression, Staff Diversity. All of the patients were asked to fill out the questionnaire (with written informed consents at the time of discharge from the hospitals. Results: Regarding total score of patient satisfaction the ISO-certified hospitals did not show advantages over the uncertified hospitals. The total score of patients’ satisfaction ranged from 66.5 to 77.5 in. Overall, only in one ISO-certified hospital the total score of patient satisfaction representing all dimensions, was significantly higher comparing to other hospitals included in the study. Conclusion: It seems that solitary application of ISO standards could not improve patient satisfaction in hospitals affiliated to Shiraz University of Medical Sciences.

  2. On the new ISO guide on risk management terminology

    Energy Technology Data Exchange (ETDEWEB)

    Aven, Terje, E-mail: terje.aven@uis.n [University of Stavanger, Stavanger (Norway)

    2011-07-15

    A new ISO guide on risk management terminology has recently been issued. The guide provides basic vocabulary for developing a common understanding of risk assessment and risk management concepts and terms among organisations and functions, and across different application areas. It provides the foundation of, for example, the ISO 31000 standard on risk management. The guide strongly influences the risk assessment and risk management field, and its quality is thus of utmost importance. In this paper a critical review of the guide is conducted. We argue that the guide fails in several ways in producing consistent and meaningful definitions of many of the key concepts covered. A main focus is placed on the risk concept, which is defined as the effect of uncertainty on objectives, but also many other definitions are looked into, including probability, vulnerability, hazard, risk identification and risk description. Examples are used to illustrate the problems and show how they can be rectified. Although the focus is on the ISO guide, the discussion is to a large extent general. The overall aim of the paper is to contribute to the further development of the area of risk assessment and risk management by strengthening its conceptual basis.

  3. A New method of preparing iso-hardness plots

    Science.gov (United States)

    Kumar, V.; Patwardhan, A. K.

    1993-02-01

    A new approach has been used for the preparation of iso-hardness plots, which are very useful in the heat treating of metals and alloys. An alloy white iron containing ~6% Mn, ~5% Cr, and ~3% Cu was heat treated at 800,850,900,950,1000, and 1050 °C and soaked for 2, 4, 6, 8, and 10 h, respectively, at each of these temperatures followed by air cooling. It was observed that, although the hardness varied linearly with soaking time, its variation with soaking temperature was nonlinear. This can be represented as: H = 98.285e2021.33/T + (0.037 - 0.316 × 10- 4 T) . t where H is Vickers hardness; T is soaking temperature in K; and t is soaking time in seconds. The variation in hardness has been discussed in light of microstructural changes that can take place upon heat treating. A three-dimensional plot of hardness, soaking temperature, and time was prepared based on the above equation. It revealed that the variation in hardness on heat treating can be represented as a surface instead of lines, where the change in the slope of the surface reflects the various transformations occurring in the alloy system. This iso- hardness plot, consisting of contours of constant hardness, has been prepared by projecting the above-mentioned surface on the soaking temperature- time plane. The method of selection of heat treating parameters based on this iso- hardness plot is discussed.

  4. [ISO 9001 conformity in research, teaching and rehabilitation].

    Science.gov (United States)

    Howorka, K; Kletschka, G; Pumprla, J; Thoma, H

    1998-01-01

    Quality assurance, in particular in the areas of development and production of medical devices, is one of the tasks of biomedical engineering. The interdisciplinary working group "Functional Rehabilitation and Group Education, Vienna" is committed to the development and implementation of group education models on three levels: (1) direct education/instruction of patients with chronic diseases, (2) university research and teaching, and (3) development of technical aids for rehabilitation and the means for disseminating group education models in rehabilitation and therapy. Major aims were, by generating conformity with ISO 9001 standards, to achieve greater transparency and process optimization with very small resources in university (teaching, research, technical aids) and extra-university (rehabilitation) areas. A secondary aim was the establishment of interdisciplinary (clinical and biomedical) cooperation at university level. In all main areas (research, teaching and group education/instruction), ISO 9001-conformity was achieved by our activities on three methodological levels: (1) description and analysis of processes, (2) use of ISO 9001 standards for evaluating internal processes, and (3) optimization measures. The following article contains relevant elements of the quality manual and quality assurance system, and offers a typical example of innovative cooperation between medicine and medical engineering.

  5. ISO 14649 (STEP-NC: New Standards for CNC Machining

    Directory of Open Access Journals (Sweden)

    Yusri Yusof

    2010-06-01

    Full Text Available The changing economic climate has made global manufacturing a growing reality over the last decade, forcing companies from east and west and all over the world to collaborate beyond geographic boundaries in the design, manufacture and assemble of products. The ISO10303 and ISO14649 Standards (STEP and STEP-NC have been developed to introduce interoperability into manufacturing enterprises so as to meet the challenge of responding to production on demand. The paper focuses on the use of this new standard to address the process planning and machining of turn/mill discrete components. Due to the complexity of programming these machines there is a need to model their process capability to improve the interoperable manufacturing capability in places such as turning centres. Finally a proposed computational environment for a STEP-NC compliant system for turning operations (SCSTO is described. And supported by the specification of information models and constructed using a structured methodology and object-oriented methods. SCSTO was developed to generate a Part 21 file based on machining features to support the interactive generation of process plans utilizing feature extraction. A case study component has been developed to prove the concept for using the milling and turning parts of ISO14649 to provide a turn-mill CAD/CAPP/CAM environment.

  6. ISO出版ISO 11960技术勘误

    Institute of Scientific and Technical Information of China (English)

    方伟

    2006-01-01

    国际标准化组织第67技术委员会第5分委会于2004年出版了ISO 11960:2004《石油天然气工业-油气井套管或油管用钢管》第2版,2005年7月,美国石油学会以等同采用该国际标准的形式制定并出版了API Spec 5CT《套管和油管规范》第8版,该标准自2006年1月1日开始实施.在标准实施过程中,发现了标准文本中的部分技术错误.ISO/TC67/SC5于2006年5月15日出版了对ISO11960:2004的技术勘误,现将该勘误内容刊载如下,供该标准使用人员参考。

  7. ISO Observations of Titan with SWS/Grating

    Science.gov (United States)

    Coustenis, A.; Encrenaz, Th.; Salama, A.; Lellouch, E.; Gautier, D.; Kessler, M. F.; de Graauw, Th.; Samuelson, R. E.; Bjoraker, G.; Orton, G.

    ISO observations of Titan in the thermal infrared were performed around January the 10th using the Grating mode of the Short Wavelength Spectrometer (SWS) in the 2-45 um region. Titan was at Eastern Elongation with respect to Saturn, showing its leading hemispbere as viewed from the Earth. The resolving power of the SWS varies from one AOT Band to the other, but is roughly on the order of 1500-3000 in the Grating mode, giving spectral resolutions for the 200-1500 cm-1 region of 0.1 - 0.8 cm-1 in the 7-45 um and 1 - 2 cm-1 in the 2.45-5 um region. The observed ranges on Titan are associated to signal-to-noise ratios of 15-80 and as much as 200. Hereafter we concentrate on the 7-45 micron range of Titan's spectrum (part of which is shown in Fig. 1) as observed by ISO/SWS. The spectrum of Titan in the 200-1500 cm-1 range was first observed as a whole by IRIS ahoard Voyager 1 in November 1980, allowing the detection in the stratosphere of numerous hydrocarbons, some nitriles and an oxygen compound (see e.g. Hanel et al. 1981). The spectral resolution of IRIS was about 4.3 cm-1, 5-20 times lower than ISO/SWS in this same range.

  8. ISO 14051: A new era for MFCA implementation and research

    Directory of Open Access Journals (Sweden)

    Katherine L. Christ

    2016-01-01

    Full Text Available Material flow cost accounting (MFCA is a tool designed to encourage eco-efficiency in organizations by focusing on a reduction in use of materials and related improvements in economic performance of corporations. It provides a way to identify win–win situations where monetary and environmental performance can both be improved. But take-up by business is slow, which seems to go against the notion of strong competition driving economic performance. A recent standard, ISO 14051, has been produced by the International Organization for Standardization, and could bring substantial change to MFCA implementation and research. Drawing on Rogers (2003 theory of diffusion of innovation, and with a focus on the first two stages of the innovation-decision process, knowledge and persuasion, this study sought to analyze MFCA and predict how the 2011 release of ISO 14051 might be expected to influence take-up of MFCA by business, and what this might mean for future research. The analysis revealed that, when combined with ISO involvement, MFCA is well placed in terms of Rogers’ theory, with the future likely to see increased diffusion of MFCA and, as adoption rates increase, more opportunities for research in this area. Specific areas identified as a result of the analysis include: the introduction of new research methods, the need for theoretically informed research, and the potential to address new research questions previously considered impractical.

  9. NF-κB involved in transcription enhancement of TGF-β1 induced by Ox-LDL in rat mesangial cells

    Institute of Scientific and Technical Information of China (English)

    兰洋; 周钦; 吴兆龙

    2004-01-01

    Background To determine the binding activity of nuclear factor-KB (NF-κB) and the transcription of transforming growth factor-βl (TGF-β1) induced by oxidized Iow density lipoprotein (Ox-LDL) in rat mesangial cells and to elucidate the mechanism of renal injury of Ox-LDL Methods NF-κB binding activity was measured by gel shift assay in mesangial cells with or without inducement of Ox-LDL. Protein kinase inhibitors and activtors were then used to determine the signal transduction pathways. In this course IκB protein expression was analyzed by Westerm blot assay. TGF-β1 mRNA was measured in mesangial cells exposed to Ox-LDL by RT-PCR assay. TGF-β1 promoter from -1551 to+57 were constructed into a pGL3-Basic vector with a luciferase reporting gene. A putative binding site of NF-KB was mutated. The wild and mutant promoters activity was analyzed by transfection into mesangial cells. Results NF-KB was activated by Ox-LDL persistently and rebounded in the early period. Ox-LDL induced NF-κB activation in a dose dependent way. It also induced IκB degradation in 2 hours and resumed to normal levels. NF-κB activation was not alleviated by inhibitors of protein kinase A ( PKA), extracellular signal-regulated kinase (ERK), and p38 MAP kinase (p38MAPK). Inhibitors of protein kinase C (PKC) and proteinsome inhibited the enhancement of NF-KB binding activity. Ox-LDL induced the transcription of TGF-βI in a time and dose dependent manner. Mutation of the putative binding site of NF-κB reduced the activity of TGF-β1 promoter. Conclusion Ox-LDL induced activation of NF-KB persistently. It was probably regulated by the degradation of IκB mediated by PKC pathway. NF-KB may be involved in the enhancement of TGF-βI induced by Ox-LDL in rat mesangial cells.

  10. NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqr activation in echiuran worm Urechis unicinctus

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xiaolong; Qin, Zhenkui; Li, Xueyu; Ma, Xiaoyu; Gao, Beibei; Zhang, Zhifeng, E-mail: zzfp107@ouc.edu.cn

    2016-06-15

    Highlights: • Sulfide activates sqr transcription against respiratory toxicity in Urechis unicinctus. • Sulfide increases expressions and activities of NF1, Sp1 and HSF1 in a time-dependent manner. • NF1 and Sp1 participate in both basal and early sulfide-induced sqr transcription. • HSF1 functions more significantly than NF1 and Sp1 in sulfide-induced sqr transcription. • Transcription factors NF1, Sp1 and HSF1 enhance sqr promoter activity synergistically. - Abstract: Background: Sulfide is a well-known environmental toxic substance. Mitochondrial sulfide oxidation is a main mechanism of sulfide detoxification in organisms, and sulfide: quinone oxidoreductase (SQR) is a key enzyme which is involved in transferring electrons from sulfide to ubiquinone and converting sulfide into thiosulfate. Previous studies have revealed the SQR-mediated mitochondrial sulfide oxidation exists in the echiuran worm Urechis unicinctus, and its sqr mRNA level increased significantly when the worm is exposed to sulfide. In this study, we attempt to reveal the synergistic regulation of transcription factors on sulfide-induced sqr transcription in U. unicinctus. Methods: ChIP and EMSA were used to identify the interactions between sqr proximal promoter (from −391 to +194 bp) and transcription factors NF1 (nuclear factor 1) and Sp1 (specificity protein 1). Site-directed mutation and transfection assays further revealed their binding sites and synergistic roles of HSF1, NF1 and Sp1 in the sqr transcription. When U. unicinctus were exposed to 150 μM sulfide, the expression levels and nuclear contents of NF1 and Sp1 were examined by Western blotting, and the binding contents between NF1 or Sp1 and the sqr promoter were also detected by ChIP. Results: Transcription factors NF1 and Sp1 were confirmed to interact with the sqr proximal promoter, and their binding sites were identified in −75 to −69 bp for NF1 and −210 to −201 bp for Sp1. Transfection assays showed mutation

  11. Clinicopathologic implications of NF1 gene alterations in diffuse gliomas.

    Science.gov (United States)

    Vizcaíno, M Adelita; Shah, Smit; Eberhart, Charles G; Rodriguez, Fausto J

    2015-09-01

    Recent studies have identified somatic alterations in the gene encoding for neurofibromin (NF1) in a subset of glioblastoma (GBM), usually associated with the mesenchymal molecular subtype. To understand the significance of NF1 genetic alterations in diffuse gliomas in general, we evaluated public databases and tested for NF1 copy number alterations in a cohort using fluorescence in situ hybridization. NF1 genetic loss (homozygous NF1 deletions or mutations with predicted functional consequences) was present in 30 (of 281) (11%) GBM and 21 (of 286) (7%) lower-grade gliomas in The Cancer Genome Atlas data. Furthermore, NF1 loss was associated with worse overall and disease-specific survival in the lower-grade glioma, but not GBM, Group in The Cancer Genome Atlas cohort. IDH1 or 2 mutations co-existed in lower-grade gliomas with NF1 loss (36%) but not in GBM. In our cohort studied by fluorescence in situ hybridization, NF1/17q (n = 2) or whole Ch17 (n = 3) losses were only identified in the GBM group (5/86 [6%]). Tumors with NF1/Ch17 loss were predominantly adult GBM (4/5); lacked EGFR amplification (0/4), strong p53 immunolabeling (1/5), or IDH1 (R132H) protein expression (0/5); but expressed the mesenchymal marker podoplanin in 4/5. NF1 genetic loss occurs in a subset of diffuse gliomas, and its significance deserves further exploration.

  12. Neurofibromatosis type 1 (NF1) and associated tumors.

    Science.gov (United States)

    Rosenbaum, T; Wimmer, K

    2014-11-01

    Neurofibromatosis type 1 (NF1) is a frequent neurocutaneous syndrome that predisposes for various benign and malignant tumors. Most characteristic are neurofibromas which occur in almost all NF1 patients at some point in lifetime. Although neurofibromas are benign tumors they can be disfiguring and plexiform neurofibromas may progress to malignant peripheral nerve sheath tumors. Overall survival rates of patients with these malignant tumors are poor. Other neoplasias frequently observed in NF1 patients are pilocytic astrocytomas, gastrointestinal stromal tumors, pheochromocytomas and juvenile myelomonocytic leukemia. Several other tumors have been reported in NF1 patients but it is unclear if there is a true association between the particular tumor type and NF1. Some of these tumors might be caused by a rare recessively inherited childhood cancer syndrome termed constitutive mismatch repair deficiency syndrome which shows certain phenotypic overlap with NF1 but includes a broad spectrum of tumors which usually do not occur in NF1. The development of NF1-associated tumors is largely explained by the underlying defect of the NF1 gene which results in activation of the RAS proto-oncogene- a key mechanism of tumorigenesis. Several downstream effectors of activated RAS as well as cooperating molecular pathways have been identified. These insights provide the basis to develop novel targeted treatment strategies which are urgently needed to improve the outcome for patients with NF1-associated malignancies.

  13. Clinicopathologic implications of NF1 gene alterations in diffuse gliomas☆

    Science.gov (United States)

    Vizcaíno, M. Adelita; Shah, Smit; Eberhart, Charles G.; Rodriguez, Fausto J.

    2015-01-01

    Summary Recent studies have identified somatic alterations in the gene encoding for neurofibromin (NF1) in a subset of glioblastoma (GBM), usually associated with the mesenchymal molecular subtype. To understand the significance of NF1 genetic alterations in diffuse gliomas in general, we evaluated public databases and tested for NF1 copy number alterations in a cohort using fluorescence in situ hybridization. NF1 genetic loss (homozygous NF1 deletions or mutations with predicted functional consequences) was present in 30 (of 281) (11%) GBM and 21 (of 286) (7%) lower-grade gliomas in The Cancer Genome Atlas data. Furthermore, NF1 loss was associated with worse overall and disease-specific survival in the lower-grade glioma, but not GBM, Group in The Cancer Genome Atlas cohort. IDH1 or 2 mutations co-existed in lower-grade gliomas with NF1 loss (36%) but not in GBM. In our cohort studied by fluorescence in situ hybridization, NF1/17q (n = 2) or whole Ch17 (n = 3) losses were only identified in the GBM group (5/86 [6%]). Tumors with NF1/Ch17 loss were predominantly adult GBM (4/5); lacked EGFR amplification (0/4), strong p53 immunolabeling (1/5), or IDH1 (R132H) protein expression (0/5); but expressed the mesenchymal marker podoplanin in 4/5. NF1 genetic loss occurs in a subset of diffuse gliomas, and its significance deserves further exploration. PMID:26190195

  14. Detection and clinical implication of TNF-α and 8-isoPG in Exhaled Breath Condensate of Asthmatic Patients%哮喘患者呼出气冷凝液中TNF-α、8-isoPG检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    张建辉; 陶一江; 陈晓笑; 马航; 陈金亮

    2011-01-01

    目的 了解哮喘患者呼出气冷凝液(EBC)中TNF-α、8-isoPG的含量,并观察激素治疗对其水平的影响.方法 选择轻、中度哮喘患者38例和健康对照组25例,其中轻度哮喘组20例、中度哮喘组18例.轻度哮喘组予以吸入激素治疗一个 月.采用EHSA双抗体夹心法测定EBC中TN F-α、8 -isoPG的含量.结果 哮喘组EBC中TNF-α、8-isoPG的含量显著高于对照组(P<0.05).结论 哮喘患者EBC中TNF-α、8-isoPG的含量增高与哮喘严重程度有关,可作为检测哮喘气道炎症反应和氧化应激的客观指标.吸入糖皮质激素治疗后TNF-α、8-isoPG含量无显著变化,提示糖皮质激素不能完全有效控制哮喘患者气道炎症反应和氧化应激损伤.%Objective To examine the levels of TNF-α and 8-isoPC in exhaled breath condensates (EBC) in patients with asthma, and to investigate the effect of corticosteroid treatment. Methods 38 patients with mild to moderate asthma and 25 notnal controls were included in the study. Among 38 patients, 20 cases were mild asthma group and 18 cases were moderate asthma group. The mild asthma group were treated by Corticosteroid for one month. TNF-α and 8-isoPG in EBC were assayed by enzyme linked immunosorbenl assay. Results ①The TNF-α and 8-isoPG levels in EBC of the asthma group were higher than those of control group (P 0.05) in the TNF-a and 8-isoPC levels in EBC of the mild asthma group before and after therapy. Conclusion TNF-α and 8-isoPC levels in EBC are associated with the severity of asthma, and implicating TNF-α and 8-isoPG may be useful in monitoring airway inflammation and airway oxidative stress in asthma. ICS treatment had no significant difference of the TNF-α and 8-isoPG levels, which suggests that the ICS could not have an impact on airway inflammation and oxidative stress completely.

  15. The Relation of the Total Quality Management and ISO 9000%TQM与ISO 9000的关系

    Institute of Scientific and Technical Information of China (English)

    王志坚

    2003-01-01

    随着经济全球化和WTO的到来,质量管理的对象已从原来"商品、服务的质量"扩大至"经营系统的质量".因此,必须提高TQM(综合质量管理)水平,实施ISO 9000,使我国企业质量管理的管理方法规范并与国际接轨,管理水平达到世界先进水平.文中介绍了综合质量管理的产生,运作需注意的问题,ISO 9000质量标准和2000版标准的主要特点,最后讨论了TQM和ISO的关系.

  16. NF-κB和HPA在口腔鳞状细胞癌中的表达及意义%Expression and significance of NF-κB and HPA in oral squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    吴祥冰; 王荣艳; 田涛; 徐佳; 朱声荣

    2011-01-01

    目的:应用组织微阵列技术,检测口腔鳞癌中核转录因子KB(NF-κB)和乙酰肝素酶(HPA)的表达,探讨两者之间的关系及其临床意义.方法:应用组织微阵列技术和免疫组化方法,检测146例口腔鳞癌、48例正常口腔黏膜中NF-KB和HPA的表达.应用SPSS13.0软件包对数据进行χ2检验和Spearman相关分析.结果:NF-KB和HPA在口腔鳞癌中的阳性表达率分别为69.2%和63.7%,显著高于正常口腔黏膜组(P<0.05).NF-κB和HPA在口腔鳞癌中的表达与肿瘤的分化程度、临床分期和有无区域淋巴结或远处转移相关(P<0.05),而与患者性别、年龄、肿瘤发生部位无关(P>0.05).NF-κB的表达与HPA的表达呈正相关(r=0.7301,P<0.05).结论:NF-κB与HPA协同作用.促进口腔鳞癌的发生、发展.%PURPOSE: To detect the expression of NF-κB and HPA in oral squamous cell carcinoma (OSCC) by tissue microarray assay and investigate their clinical significance. METHODS: Tissue microarray assay and immunohistochemistry were applied to detect the expression of NF-κB and HPA in 146 cases of OSCC and 48 cases of normal control. Chisquare test and Spearman rank correlation were performed using SPSS 13.0 software package for statistics. RESULTS:The positive rates of NF-κB and HPA were 69.2% and 63.7% ,respectively.There was significant difference compared with normal oral mucosa (P<0.05). The expression of NF-κB and HPA in OSCC was significantly correlated with histological differentiation, clinical stage and lymph node or distant metastasis (P<O.05), but not with sex and age of the patients or site of the tumor (P>0.05).The expression of NF-κB had a significant positive correlation with the expression of HPA in OSCC (r=0.7301,P<0.05). CONCLUSION: The expression of NF-κB and HPA may have synergistic effect on the carcinogenesis and progression of OSCC.

  17. Role of autocrine osteopontin in promoting multiple functions of murine Nf1+/-osteoclast%自分泌骨桥蛋白在Nf1+/-小鼠破骨细胞功能增强中的作用

    Institute of Scientific and Technical Information of China (English)

    李会杰; 刘亚玲; 井永敏; 张英泽; 王振昊; 闫金成

    2013-01-01

    Objective To detect the osteopontin (OPN) autocrine function of the osteoclasts in neurofibromatosis type 1 heterozygote (Nfl+/-) and wild type (Nfl+/+) mice.Test the osteoclasts function of neurofibromatosis type 1 heterozygote (Nfl+/-) and wild type (Nil+/+) mice with exogenous neutralizing OPN antibody,analysis the role of autocrine OPN in the hyperfunction of osteoclast in neurofibromatosis type 1.Methods Culture the low density bone marrow cells from Nfl heterozygote (Nfl+/-) and wild type (Nfl+/+) mice (4-6 weeks old) with macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand(RANKL),Measure.the OPN concentration in osteoclast culture superenant with ELISA.Culture the low density bone marrow cells from Nf1+/-and Nf1+/+ mice with or without exogenous neutralizing antibody for OPN.The function of osteoclasts and osteoclast progenitors in formation,migration,adhesion,and bone absorption were tested.Results A significantly higher concentration of OPN was detected in the Nf1+/-osteoclast culture media as compared to that of wild type.In control,Osteoclast functions,including migration,adhesion,and bone resorption of Nf1 +/-were higher than that of wild type.Addition OPN neutralizing antibody to the Nf1+/-OCL significantly reduced OCL formation.Neutralizing OPN antibody diminished both wild type and Nf1+/-OCL adhensiontion,Anti-OPN minimized OCL migration in both wild type and Nf1 +/-OCL cultures as measured by the transwell assays.Neutralizing OPN antibody diminished both wild type and Nf1+/-OCL pit formation,P>0.05 for comparing Nfl+/-vs.wild type OCLs with anti-OPN antibody.Conclusion The hyperfunction of osteoclast in Nf1 heterozygote is related with autocrine osteopontin,inhibition of OPN may be an effective treatment for bone destruction of neurofibromatosis type 1.%目的 研究体外培养的Nf1+/-小鼠破骨细胞合成、分泌骨桥蛋白(osteopontin,OPN)的能力,应用OPN中和抗体抑制破骨细胞分泌的OPN,测

  18. Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.

    Directory of Open Access Journals (Sweden)

    Bin Yi

    Full Text Available AIMS: To investigate if nuclear NF-κB p65 expression in ex vivo isolated peripheral blood mononuclear cells correlates with urinary MCP-1 or RANTES and the severity of type 2 diabetic nephropathy. METHODS: According to their urinary albumin-to-creatinine ratio (uACR, 107 patients with type 2 diabetes (eGFR >60 ml/min were divided into normal albuminuria group (DN0 group, 38 cases, microalbuminuria group (DN1 group, 38 cases, and macroalbuminuria group (DN2 group, 31 cases, compared with matched healthy normal control group (NC group, 30 cases. Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting. Real-time quantitative polymerase chain reaction was used to detect NF-κB p65 mRNA expression and ELISA assay was used to detect the levels of urinary MCP-1 and RANTES. RESULTS: Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group. In particular, the increase of nuclear NF-κB p65 protein and NF-κB p65 mRNA expressions, urinary MCP-1/Cr and RANTES/Cr all correlated with the severity of type 2 diabetic nephropathy as indicated by the increase in uACR. Pearson correlation analysis indicated that both urinary MCP-1/Cr and RANTES/Cr were positively correlated with nuclear NF-κB p65 protein or NF-κB p65 mRNA levels. Stepwise multiple regression analysis showed that nuclear NF-κB p65 protein or NF-κB p65 mRNA was an independent variable for urinary MCP-1/Cr, and MCP-1/Cr and RANTES/Cr were two independent variables for uACR. CONCLUSION: Our research demonstrates that nuclear NF-κB p65 protein and mRNA expressions in ex vivo isolated peripheral blood mononuclear cells well correlate with urinary MCP-1/Cr, RANTES/Cr and the severity of type 2 diabetic nephropathy.

  19. A practical synthesis of long-chain iso-fatty acids (iso-C12-C19) and related natural products.

    Science.gov (United States)

    Richardson, Mark B; Williams, Spencer J

    2013-01-01

    A gram-scale synthesis of terminally-branched iso-fatty acids (iso-C12-C19) was developed commencing with methyl undec-10-enoate (methyl undecylenate) (for iso-C12-C14) or the C15 and C16 lactones pentadecanolide (for iso-C15-C17) and hexadecanolide (for iso-C18-C19). Central to the approaches outlined is the two-step construction of the terminal isopropyl group through addition of methylmagnesium bromide to the ester/lactones and selective reduction of the resulting tertiary alcohols. Thus, the C12, C17 and C18 iso-fatty acids were obtained in three steps from commercially-available starting materials, and the remaining C13-C16 and C19 iso-fatty acids were prepared by homologation or recursive dehomologations of these fatty acids or through intercepting appropriate intermediates. Highlighting the synthetic potential of the iso-fatty acids and various intermediates prepared herein, we describe the synthesis of the natural products (S)-2,15-dimethylpalmitic acid, (S)-2-hydroxy-15-methylpalmitic acid, and 2-oxo-14-methylpentadecane.

  20. A practical synthesis of long-chain iso-fatty acids (iso-C12–C19 and related natural products

    Directory of Open Access Journals (Sweden)

    Mark B. Richardson

    2013-09-01

    Full Text Available A gram-scale synthesis of terminally-branched iso-fatty acids (iso-C12–C19 was developed commencing with methyl undec-10-enoate (methyl undecylenate (for iso-C12–C14 or the C15 and C16 lactones pentadecanolide (for iso-C15–C17 and hexadecanolide (for iso-C18–C19. Central to the approaches outlined is the two-step construction of the terminal isopropyl group through addition of methylmagnesium bromide to the ester/lactones and selective reduction of the resulting tertiary alcohols. Thus, the C12, C17 and C18 iso-fatty acids were obtained in three steps from commercially-available starting materials, and the remaining C13–C16 and C19 iso-fatty acids were prepared by homologation or recursive dehomologations of these fatty acids or through intercepting appropriate intermediates. Highlighting the synthetic potential of the iso-fatty acids and various intermediates prepared herein, we describe the synthesis of the natural products (S-2,15-dimethylpalmitic acid, (S-2-hydroxy-15-methylpalmitic acid, and 2-oxo-14-methylpentadecane.

  1. The NF1 gene in tumor syndromes and melanoma.

    Science.gov (United States)

    Kiuru, Maija; Busam, Klaus J

    2017-02-01

    Activation of the RAS/MAPK pathway is critical in melanoma. Melanoma can be grouped into four molecular subtypes based on their main genetic driver: BRAF-mutant, NRAS-mutant, NF1-mutant, and triple wild-type tumors. The NF1 protein, neurofibromin 1, negatively regulates RAS proteins through GTPase activity. Germline mutations in NF1 cause neurofibromatosis type I, a common genetic tumor syndrome caused by dysregulation of the RAS/MAPK pathway, ie, RASopathy. Melanomas with NF1 mutations typically occur on chronically sun-exposed skin or in older individuals, show a high mutation burden, and are wild-type for BRAF and NRAS. Additionally, NF1 mutations characterize certain clinicopathologic melanoma subtypes, specifically desmoplastic melanoma. This review discusses the current knowledge of the NF1 gene and neurofibromin 1 in neurofibromatosis type I and in melanoma.

  2. Role of Merlin/NF2 in mTOR Signaling and Meningioma Growth

    Science.gov (United States)

    2012-04-01

    Nonidet p - 40 (NP- 40 ) containing calyculin (50 nmol/L; Cell Signaling Technology), 1...IP kinase In vitro assay EGF-stimulated arachnoid cells 0 20 40 60 80 100 120 con RNAi NF2 RNAi A ve ra g e re la ti ve A kt S 47 3 p h o...R T - P R T pAKT S473 pp70 S6K T389 Akt pAKT S473 (long exposure) 1 h 24 h BA pAkt S473 DC p70 S6K 1 h 24 h 0 10 20 30 40

  3. 78 FR 67357 - New England Power Generators Association, Inc. v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2013-11-12

    ... Energy Regulatory Commission New England Power Generators Association, Inc. v. ISO New England Inc... Association, Inc. (NEPGA or Complainant) filed a complaint against ISO New England, Inc. (ISO-NE or Respondent). NEPGA alleges that the provisions of the ISO-NE Tariff that set capacity prices during...

  4. 建材行业贯彻ISO 9000及同时实施ISO 14000系列标准--从实践到发展

    Institute of Scientific and Technical Information of China (English)

    方文时

    2002-01-01

    ISO 9000系列标准是一套既有科学理论基础、又有实践背景的质量管理和质量保证的国际标准.本文首先对ISO 9000系列标准产生的历史背景及国内外发展状况进行了回顾;然后以天津三联公司为例,对企业实施ISO 9000系列标准的组织程序、实施效果及存在的问题进行了分析.最后对建材行业同时实施ISO 9000及ISO 14000系列标准提出几点建议.

  5. The Role of NF1 in Memory Retrieval

    Science.gov (United States)

    2014-09-01

    AD_________________ Award Number: W81XWH-10-1-0450 TITLE: T R NF1 M PRINCIPAL... NF1 M R 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER YI ZHONG 5e. TASK NUMBER E-Mail: zhongyi...ABSTRACT This
three‐year
grant
supports
our
efforts
in
studying
a
role
of
the
neurofibromatosis
type
 1
( NF1 )
gene
in
mediating
retrieval
of
long‐term

  6. Nerve Degeneration and Regeneration Associated with NF1 Tumors

    Science.gov (United States)

    2014-09-01

    Associated with NF1 Tumors PRINCIPAL INVESTIGATOR: David F. Muir CONTRACTING ORGANIZATION: University of Florida...NUMBER Nerve Degeneration and Regeneration Associated with NF1 Tumors 5b. GRANT NUMBER W81XWH-11-1-0145 5c...for  the   eradication  of  PNSTs.    Our  preliminary  studies  indicate  that  PDT  effectively  kills  human   NF1

  7. Genotyping of PCR-based polymorphisms and linkage-disequilibrium analysis at the NF1 locus

    Energy Technology Data Exchange (ETDEWEB)

    Purandare, S.M.; Viskochil, D.H.; Cawthon, R. [Univ. of Utah, Salt Lake City, UT (United States)] [and others

    1996-07-01

    Six polymorphism across the NF1 gene have been adapted for genotyping through application of PCR-based assays. Three exon-based polymorphisms - at positions 702, 2034, and 10647 in the NF1 cDNA - were genotyped by mutagenically separated PCR (MS-PCR). A fourth polymorphism, DV1.9, is an L1 insertion element in intron 30, and the other two polymorphisms, GXAlu and EVI-20, are short tandem repeats in intron 27b. All the polymorphisms were evaluated in a cohort of 110 CEPH individuals who previously had been analyzed by use of eight RFLPs at the NF1 locus. Pairwise linkage-disequilibrium analyses with the six PCR-based polymorphisms and their flanking markers demonstrated disequilibrium between all tested loci. Genotypes of the four diallelic polymorphisms (702, 2034, 10647, and DV1.9) were also evaluated in cohorts from the CEPH, African, and Japanese populations. The CEPH and Japanese cohorts showed similar heterozygosities and linkage-disequilibrium coefficients. The African cohort showed a higher degree of heterozygosity and lower linkage-disequilibrium values, compared with the CEPH and Japanese cohorts. 36 refs., 2 figs., 3 tabs.

  8. Characterization of NF-kB-mediated inhibition of catechol-O-methyltransferase

    Directory of Open Access Journals (Sweden)

    Conrad Matthew

    2009-03-01

    Full Text Available Abstract Background Catechol-O-methyltransferase (COMT, an enzyme that metabolizes catecholamines, has recently been implicated in the modulation of pain. Specifically, low COMT activity is associated with heightened pain perception and development of musculoskeletal pain in humans as well as increased experimental pain sensitivity in rodents. Results We report that the proinflammatory cytokine tumor necrosis factor α (TNFα downregulates COMT mRNA and protein in astrocytes. Examination of the distal COMT promoter (P2-COMT reveals a putative binding site for nuclear factor κB (NF-κB, the pivotal regulator of inflammation and the target of TNFα. Cell culture assays and functional deletion analyses of the cloned P2-COMT promoter demonstrate that TNFα inhibits P2-COMT activity in astrocytes by inducing NF-κB complex recruitment to the specific κB binding site. Conclusion Collectively, our findings provide the first evidence for NF-κB-mediated inhibition of COMT expression in the central nervous system, suggesting that COMT contributes to the pathogenesis of inflammatory pain states.

  9. Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB pathway

    Institute of Scientific and Technical Information of China (English)

    Yang XUE; Ying WANG; De-chun FENG; Bao-guo XIAO; Ling-yun XU

    2008-01-01

    Aim: Microglial activation has been implicated in many neurological diseases. In this study, we examined the effects of tetrandrine (TET), a major pharmacologi-cally-active compound of Chinese herb Stephania tetrandra S Moore on micro-glial activation. Methods: The microglia pretreated with or without TET were activated by lipoopolysaccharide (LPS) in vitro. Nitric oxide (NO) release, superox-ide anion (O2-) generation, as well as TNF-α and intedeukin-6 (IL-6) production by microglia were measured afterwards. Electrophoretic mobility shift assay was performed to determine whether NF-κB activity in microglia was affected by TET treatment. Results: We found that TET inhibited the LPS-induced activation of microglia by decreasing the production of NO and O2-, consequently affecting the release of TNF-α and IL-6 in LPS-induced microglial activation. Such suppressive effect was accompanied by inhibiting transcription factor NF-κB activation. Conclusion: Our results suggest that TET might modulate LPS-induced microglial activation by inhibiting the NF-κB-mediated release of inflammatory factors.

  10. Isolation of anteiso-C17, iso-C17, iso-C16, and iso-C15 bacillomycin D from Bacillus amyloliquefaciens SD-32 and their antifungal activities against plant pathogens.

    Science.gov (United States)

    Tanaka, Keijitsu; Ishihara, Atsushi; Nakajima, Hiromitsu

    2014-02-19

    Two new cyclic lipopeptides (3 and 4) were isolated from the culture filtrate of Bacillus amyloliquefaciens strain SD-32, together with two known metabolites, iso-C15 and iso-C16 bacillomycin D (1 and 2). Spectroscopic and chemical analyses identified the structures of the new compounds 3 and 4 as anteiso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-14-methylhexadecanoic acid) and iso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-15-methylhexadecanoic acid), respectively. The absolute configuration of C-3 in the β-amino fatty acid was determined to be R on the basis of the CD spectrum of its dinitrophenyl-p-methoxyaniline derivative. The activities of compounds 1-4 were evaluated against 13 plant pathogens: the activities of anteiso- and iso-C17 bacillomycin D (3 and 4) were almost the same and stronger than those of iso-C15 and iso-C16 bacillomycin D (1 and 2); iso-C15 bacillomycin D (1) was weakest. Compounds 2-4 inhibited the growth of all fungi tested; however, Pythium aphanidermatum was not inhibited at all by any of the compounds. Furthermore, compounds 1-4 at concentrations of 80, 40, 30, and 30 μM, respectively, inhibited completely the Botrytis cinerea infection in cucumber leaf.

  11. Isorhamnetin augments the anti-tumor effect of capeciatbine through the negative regulation of NF-κB signaling cascade in gastric cancer.

    Science.gov (United States)

    Manu, Kanjoormana A; Shanmugam, Muthu K; Ramachandran, Lalitha; Li, Feng; Siveen, Kodappully Sivaraman; Chinnathambi, Arunachalam; Zayed, M E; Alharbi, Sulaiman Ali; Arfuso, Frank; Kumar, Alan Prem; Ahn, Kwang Seok; Sethi, Gautam

    2015-07-10

    Development of drug resistance to standard chemotherapy is a common phenomenon that leads to poor prognosis in patients. Thus, novel agents that can attenuate chemoresistance are urgently needed. Therefore, we analyzed whether isorhamnetin (IH), a 3'-O-methylated metabolite of quercetin, can enhance the potential efficacy of capecitabine in gastric cancer. The potential effect of IH on viability was analyzed by MTT assay, apoptosis by flow cytometric analysis, and NF-κB activation by DNA binding as well as Western blot assays. The in vivo effect of IH was also examined on the growth of subcutaneously implanted tumors in nude mice. IH inhibited the viability, potentiated the apoptotic effects of capecitabine, abrogated NF-κB activation, and suppressed the expression of various NF-κB regulated gene products in tumor cells. In a gastric cancer xenograft model, administration of IH alone (1 mg/kg body weight, i.p.) significantly suppressed the tumor growth alone as well as in combination with capecitabine. IH further reduced NF-κB activation and the expression of various proliferative and oncogenic biomarkers in tumor tissues. Overall, our results demonstrate that IH can significantly enhance the anti-tumor effects of capecitabine through the negative regulation of NF-κB regulated oncogenic genes.

  12. Veronicastrum axillare Alleviates Ethanol-Induced Injury on Gastric Epithelial Cells via Downregulation of the NF-kB Signaling Pathway

    Science.gov (United States)

    Xu, Yan-shan; Chen, Gang; Guo, Yan; Wang, Dan-yi; Meng, Gui-bin

    2017-01-01

    We used human gastric epithelial cells (GES-1) line in an ethanol-induced cell damage model to study the protective effect of Veronicastrum axillare and its modulation to NF-κB signal pathway. The goal was to probe the molecular mechanism of V. axillare decoction in the prevention of gastric ulcer and therefore provide guidance in the clinical application of V. axillare on treating injuries from chronic nephritis, pleural effusion, gastric ulcer, and other ailments. The effects of V. axillare-loaded serums on cell viability were detected by MTT assays. Enzyme-linked immunosorbent assay (ELISA) and Real-Time PCR methods were used to analyze the protein and mRNA expression of TNF-α, NF-κB, IκBα, and IKKβ. The results showed that V. axillare-loaded serum partially reversed the damaging effects of ethanol and NF-κB activator (phorbol-12-myristate-13-acetate: PMA) and increased cell viability. The protein and mRNA expressions of TNF-α, NF-κB, IκBα, and IKKβ were significantly upregulated by ethanol and PMA while they were downregulated by V. axillare-loaded serum. In summary, V. axillare-loaded serum has significantly protective effect on GES-1 against ethanol-induced injury. The protective effect was likely linked to downregulation of TNF-α based NF-κB signal pathway. PMID:28182096

  13. ExoU activates NF-κB and increases IL-8/KC secretion during Pseudomonas aeruginosa infection.

    Directory of Open Access Journals (Sweden)

    Carolina Diettrich Mallet de Lima

    Full Text Available ExoU, a Pseudomonas aeruginosa cytotoxin injected into host cytosol by type III secretion system, exhibits a potent proinflammatory activity that leads to a marked recruitment of neutrophils to infected tissues. To evaluate the mechanisms that account for neutrophil infiltration, we investigated the effect of ExoU on IL-8 secretion and NF-κB activation. We demonstrate that ExoU increases IL-8 mRNA and protein levels in P. aeruginosa-infected epithelial and endothelial cell lines. Also, ExoU induces the nuclear translocation of p65/p50 NF-κB transactivator heterodimer as well as NF-κB-dependent transcriptional activity. ChIP assays clearly revealed that ExoU promotes p65 binding to NF-κB site in IL-8 promoter and the treatment of cultures with the NF-κB inhibitor Bay 11-7082 led to a significant reduction in IL-8 mRNA levels and protein secretion induced by ExoU. These results were corroborated in a murine model of pneumonia that revealed a significant reduction in KC secretion and neutrophil infiltration in bronchoalveolar lavage when mice were treated with Bay 11-7082 before infection with an ExoU-producing strain. In conclusion, our data demonstrate that ExoU activates NF-κB, stimulating IL-8 expression and secretion during P. aeruginosa infection, and unveils a new mechanism triggered by this important virulence factor to interfere in host signaling pathways.

  14. Trichothecin induces cell death in NF-κB constitutively activated human cancer cells via inhibition of IKKβ phosphorylation.

    Directory of Open Access Journals (Sweden)

    Jia Su

    Full Text Available Constitutive activation of the transcription factor nuclear factor-κB (NF-κB is involved in tumorigenesis and chemo-resistance. As the key regulator of NF-κB, IKKβ is a major therapeutic target for various cancers. Trichothecin (TCN is a metabolite isolated from an endophytic fungus of the herbal plant Maytenus hookeri Loes. In this study, we evaluated the anti-tumor activity of TCN and found that TCN markedly inhibits the growth of cancer cells with constitutively activated NF-κB. TCN induces G0/G1 cell cycle arrest and apoptosis in cancer cells, activating pro-apoptotic proteins, including caspase-3, -8 and PARP-1, and decreasing the expression of anti-apoptotic proteins Bcl-2, Bcl-xL, and survivin. Reporter activity assay and target genes expression analysis illustrated that TCN works as a potent inhibitor of the NF-κB signaling pathway. TCN inhibits the phosphorylation and degradation of IκBα and blocks the nuclear translocation of p65, and thus inhibits the expression of NF-κB target genes XIAP, cyclin D1, and Bcl-xL. Though TCN does not directly interfere with IKKβ kinase, it suppresses the phosphorylation of IKKβ. Overexpression of constitutively activated IKKβ aborted TCN induced cancer cell apoptosis, whereas knockdown of endogenous IKKβ with siRNA sensitized cancer cells toward apoptosis induced by TCN. Moreover, TCN showed a markedly weaker effect on normal cells. These findings suggest that TCN may be a potential therapeutic candidate for cancer treatment, targeting NF-κB signaling.

  15. Cirhin up-regulates a canonical NF-{kappa}B element through strong interaction with Cirip/HIVEP1

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Bin; Mitchell, Grant A. [Genetique Medicale, Centre de Recherche CHU Sainte-Justine, Departement de Pediatrie, Universite de Montreal, Montreal, QC (Canada); Richter, Andrea, E-mail: andrea.richter@umontreal.ca [Genetique Medicale, Centre de Recherche CHU Sainte-Justine, Departement de Pediatrie, Universite de Montreal, Montreal, QC (Canada)

    2009-11-01

    North American Indian childhood cirrhosis (NAIC/CIRH1A) is a severe autosomal recessive intrahepatic cholestasis. All NAIC patients have a homozygous mutation in CIRH1A that changes conserved Arg565 to Trp (R565W) in Cirhin, a nucleolar protein of unknown function. Subcellular localization is unaffected by the mutation. Yeast two-hybrid screening identified Cirip (Cirhin interaction protein) and found that interaction between Cirip and R565W-Cirhin was weakened. Co-immunoprecipitation of the two proteins from nuclear extracts of HeLa cells strongly supports the yeast two hybrid results. Cirip has essentially the same sequence as the C-terminal of HIVEP1, a regulator of a canonical NF-{kappa}B sequence. Since Cirip has the zinc fingers required for this interaction, we developed an in vitro assay based on this element in mammalian cells to demonstrate functional Cirhin-Cirip interaction. The strong positive effect of Cirip on the NF-{kappa}B sequence was further increased by both Cirhin and R565W-Cirhin. Importantly, the effect of R565W-Cirhin was weaker than that of the wild type protein. We observed increased levels of Cirhin-Cirip complex in nuclear extracts in the presence of this NF-{kappa}B sequence. Our hypothesis is that Cirhin is a transcriptional regulatory factor of this NF-{kappa}B sequence and could be a participant in the regulation of other genes with NF-{kappa}B responsive elements. Since the activities of genes regulated through NF-{kappa}B responsive elements are especially important during development, this interaction may be a key to explain the perinatal appearance of NAIC.

  16. Elucidating distinct roles for NF1 in melanomagenesis.

    Science.gov (United States)

    Maertens, Ophélia; Johnson, Bryan; Hollstein, Pablo; Frederick, Dennie T; Cooper, Zachary A; Messiaen, Ludwine; Bronson, Roderick T; McMahon, Martin; Granter, Scott; Flaherty, Keith; Wargo, Jennifer A; Marais, Richard; Cichowski, Karen

    2013-03-01

    BRAF mutations play a well-established role in melanomagenesis; however, without additional genetic alterations, tumor development is restricted by oncogene-induced senescence (OIS). Here, we show that mutations in the NF1 tumor suppressor gene cooperate with BRAF mutations in melanomagenesis by preventing OIS. In a genetically engineered mouse model, Nf1 mutations suppress Braf-induced senescence, promote melanocyte hyperproliferation, and enhance melanoma development. Nf1 mutations function by deregulating both phosphoinositide 3-kinase and extracellular signal-regulated kinase pathways. As such, Nf1/Braf-mutant tumors are resistant to BRAF inhibitors but are sensitive to combined inhibition of mitogen-activated protein/extracellular signal-regulated kinase kinase and mTOR. Importantly, NF1 is mutated or suppressed in human melanomas that harbor concurrent BRAF mutations, NF1 ablation decreases the sensitivity of melanoma cell lines to BRAF inhibitors, and NF1 is lost in tumors from patients following treatment with these agents. Collectively, these studies provide mechanistic insight into how NF1 cooperates with BRAF mutations in melanoma and show that NF1/neurofibromin inactivation may have an impact on responses to targeted therapies.

  17. Immortalization of human normal and NF1 neurofibroma Schwann cells.

    Science.gov (United States)

    Li, Hua; Chang, Lung-Ji; Neubauer, Debbie R; Muir, David F; Wallace, Margaret R

    2016-10-01

    Neurofibromas, which are benign Schwann cell tumors, are the hallmark feature in the autosomal dominant condition neurofibromatosis 1 (NF1) and are associated with biallelic loss of NF1 gene function. There is a need for effective therapies for neurofibromas, particularly the larger, plexiform neurofibromas. Tissue culture is an important tool for research. However, it is difficult to derive enriched human Schwann cell cultures, and most enter replicative senescence after 6-10 passages, impeding cell-based research in NF1. Through exogenous expression of human telomerase reverse transcriptase and murine cyclin-dependent kinase (mCdk4), normal (NF1 wild-type), neurofibroma-derived Schwann cells heterozygous for NF1 mutation, and neurofibroma-derived Schwann cells homozygous for NF1 mutation were immortalized, including some matched samples from the same NF1 patient. Initial experiments employed retroviral vectors, while subsequent work utilized lentiviral vectors carrying these genes because of improved efficiency. Expression of both transgenes was required for immortalization. Molecular and immunohistochemical analysis indicated that these cell lines are of Schwann cell lineage and have a range of phenotypes, many of which are consistent with their primary cultures. This is the first report of immortalization and detailed characterization of multiple human NF1 normal nerve and neurofibroma-derived Schwann cell lines, which will be highly useful research tools to study NF1 and other Schwann tumor biology and conditions.

  18. NF-kappaB Signaling in Chronic Inflammatory Airway Disease

    Directory of Open Access Journals (Sweden)

    Michael Schuliga

    2015-06-01

    Full Text Available Asthma and chronic obstructive pulmonary disease (COPD are obstructive airway disorders which differ in their underlying causes and phenotypes but overlap in patterns of pharmacological treatments. In both asthma and COPD, oxidative stress contributes to airway inflammation by inducing inflammatory gene expression. The redox-sensitive transcription factor, nuclear factor (NF-kappaB (NF-κB, is an important participant in a broad spectrum of inflammatory networks that regulate cytokine activity in airway pathology. The anti-inflammatory actions of glucocorticoids (GCs, a mainstay treatment for asthma, involve inhibition of NF-κB induced gene transcription. Ligand bound GC receptors (GRs bind NF-κB to suppress the transcription of NF-κB responsive genes (i.e., transrepression. However, in severe asthma and COPD, the transrepression of NF-κB by GCs is negated as a consequence of post-translational changes to GR and histones involved in chromatin remodeling. Therapeutics which target NF-κB activation, including inhibitors of IκB kinases (IKKs are potential treatments for asthma and COPD. Furthermore, reversing GR/histone acetylation shows promise as a strategy to treat steroid refractory airway disease by augmenting NF-κB transrepression. This review examines NF-κB signaling in airway inflammation and its potential as target for treatment of asthma and COPD.

  19. Salvia miltiorrhiza water-soluble extract, but not its constituent salvianolic acid B, abrogates LPS-induced NF-κB signalling in intestinal epithelial cells

    Science.gov (United States)

    Kim, J S; Narula, A S; Jobin, C

    2005-01-01

    Herbal medicine has become an increasing popular therapeutic alternative among patients suffering from various inflammatory disorders. The Salvia miltiorrhizae water-soluble extract (SME) have been shown to possess antioxidant and anti-inflammatory properties in vitro. However, the mechanism of action and impact of SME on LPS-induced gene expression is still unknown. We report that SME significantly abrogated LPS-induced IκB phosphorylation/degradation, NF-κB transcriptional activity and ICAM-1 gene expression in rat IEC-18 cells. Chromatin immunoprecipitation assay demonstrated that LPS-induced RelA recruitment to the ICAM-1 gene promoter was inhibited by SME. Moreover, in vitro kinase assay showed that SME directly inhibits LPS induced IκB kinase (IKK) activity in IEC-18 cells. To investigate the physiological relevance of SME inhibitory activity on NF-κB signalling, we used small intestinal explants and primary intestinal epithelial cells derived from a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-κB cis-elements (cis-NF-κBEGFP). SME significantly blocked LPS-induced EGFP expression and IκBα phosphorylation in intestinal explants and primary IECs, respectively. However, salvianolic acid B, an activate component of SME did not inhibit NF-κB transcriptional activity and IκB phosphorylation/degradation in IEC-18 cells. These results indicate that SME blocks LPS-induced NF-κB signalling pathway by targeting the IKK complex in intestinal epithelial cells. Modulation of bacterial product-mediated NF-κB signalling by natural plant extracts may represent an attractive strategy towards the prevention and treatment of intestinal inflammation. PMID:15996193

  20. Inflammatory MAPK and NF-κB signaling pathways differentiated hepatitis potential of two agglomerated titanium dioxide particles.

    Science.gov (United States)

    Chen, Jin; Zhang, Jianying; Cao, Junmei; Xia, Zongping; Gan, Jay

    2016-03-05

    TiO2 nanoparticles (TiO2NPs) consumption and deposit in liver have possible implications for hepatitis risks. Specific signal dysregulation at early inflammatory responses needs to be characterized in TiO2NP-induced hepatopathy. MAPK and NF-κB signaling pathways are known to participate in inflammation and respond sensitively to chemical agents, making them preferable biomarkers for hepatitis. In the present study, dynamic activation of MAPK and NF-κB pathways were explored by immunoblotting and NF-κB luciferase reporter assay depending on characterization of TiO2NP agglomeration in human HepG2 cells. Inflammatory and cytotoxic potential of TiO2NPs were determined by qRT-PCR and WST-1 assay. AFM and TEM analyses uncovered ultrastructure damages underlying hepatotoxicity of TiO2NPs. Rod-like rutile agglomerated smaller and induced more pronounced cytotoxicity and immunogenicity than anatase. Correspondingly, though both rutile and anatase significantly activated p38, ERK1/2 and NF-κB pathways, rutile accelerated the maximum phosphorylation of ERK1/2 and elevated the potency of IκBα phosphorylation to its bell curve shape in comparison with a lower and sigmoid type of IκBα phosphorylation for anatase. Furthermore, cell elasticity indicated by Young's modulus and adhesion force increased accompanied with mitochondria damage, contributing to signal dysregulation and hepatotoxicity. The results suggested that differential activation of MAPK and NF-κB pathways could be early predictors for distinct hepatitis risks of two agglomerated TiO2NPs.

  1. Role of NF-κB and cytokine in experimental cancer cachexia

    Institute of Scientific and Technical Information of China (English)

    Wei Zhou; Zhi-Wei Jiang; Jie Tian; Jun Jiang; Ning Li; Jie-Shou Li

    2003-01-01

    AIM: To assess the putative involvement of NF-κB and proinflammatory cytokines in the pathogenesis of cancer cachexia and the therapeutic efficacy of indomethacin (IND)on cachexia.METHODS: Thirty young male BABL/c mice were divided randomly into five groups: (a) control, (b) tumor-bearing murine were inoculated subcutaneously to induce cachexia.Saline and IND were given intraperitoneally daily for 7 days from the onset of cachexia to sacrifice. Food intake and body composition were documented, serum levels of TNFα and IL-6 and activity of NF-κB in the spleen were investigated in all animals.RESULTS: Weight loss was observed in all tumor-bearing mice. By day 16, body weights of non-tumor mice were about 72 % of healthy controls (P<0.01), and the weight of gastrocnemius was decreased by 28.7 % (P<0.01). No difference was found between groups in food intake (P>0.05).Gastrocnemius weight was increased markedly (P<0.01)body weights were not significantly elevated. Tumor-bearing caused a 2-3 fold increase in serum levels of both TNF-αand IL-6 (P<0.01). The concentration of TNF-α (P<0.05)and IL-6 (P<0.01) in tumor-bearing mice was reduced after of IL-6 was slightly elevated following treatment of IND 2.0tumor-bearing mice in comparison with controls in electrophoretic mobility shift assay (ENSA). NF-κB activity a higher NF-κB activity was observed in mice treated with CONCLUSION: Colon 26 adenocarcinoma cells can induce severe cancer cachexia experimentally, and the mechanism may be partially due to the enhanced TNF-αand IL-6 in tumor-bearing animals, which is controlled by NF-κB. Low dose of indomethacin alleviates the cachexia,decreases the activation of NF-κB and the serum levels of TNF-α and IL-6, and prevents body weight loss and muscle atrophy, while no further effect is gained by a higher dosage.

  2. An Initial Comparative Analysis of the CMMI Version 1.2 Development Constellation and the ISO 9000 Family

    Science.gov (United States)

    2009-03-01

    automobile ( ISO TS 16949), chemical (RC 14001), medical devices ( ISO 13485 ), and petroleum and natural gas ( ISO /TS 29001). These individual industry...An Initial Comparative Analysis of the CMMI Version 1.2 Development Constellation and the ISO 9000 Family David H, Kitson, KAMO Consultancy...Credentials Program 10 2.9 Adoption 11 3 Overview of the ISO 9000 Family 13 3.1 Scope of Application and Purpose 13 3.2 Background 13 3.3

  3. Ciliary neurotrophic factor activates NF-κB to enhance mitochondrial bioenergetics and prevent neuropathy in sensory neurons of streptozotocin-induced diabetic rodents.

    Science.gov (United States)

    Saleh, Ali; Roy Chowdhury, Subir K; Smith, Darrell R; Balakrishnan, Savitha; Tessler, Lori; Martens, Corina; Morrow, Dwane; Schartner, Emily; Frizzi, Katie E; Calcutt, Nigel A; Fernyhough, Paul

    2013-02-01

    Diabetes causes mitochondrial dysfunction in sensory neurons that may contribute to peripheral neuropathy. Ciliary neurotrophic factor (CNTF) promotes sensory neuron survival and axon regeneration and prevents axonal dwindling, nerve conduction deficits and thermal hypoalgesia in diabetic rats. In this study, we tested the hypothesis that CNTF protects sensory neuron function during diabetes through normalization of impaired mitochondrial bioenergetics. In addition, we investigated whether the NF-κB signal transduction pathway was mobilized by CNTF. Neurite outgrowth of sensory neurons derived from streptozotocin (STZ)-induced diabetic rats was reduced compared to neurons from control rats and exposure to CNTF for 24 h enhanced neurite outgrowth. CNTF also activated NF-κB, as assessed by Western blotting for the NF-κB p50 subunit and reporter assays for NF-κB promoter activity. Conversely, blockade of NF-κB signaling using SN50 peptide inhibited CNTF-mediated neurite outgrowth. Studies in mice with STZ-induced diabetes demonstrated that systemic therapy with CNTF prevented functional indices of peripheral neuropathy along with deficiencies in dorsal root ganglion (DRG) NF-κB p50 expression and DNA binding activity. DRG neurons derived from STZ-diabetic mice also exhibited deficiencies in maximal oxygen consumption rate and associated spare respiratory capacity that were corrected by exposure to CNTF for 24 h in an NF-κB-dependent manner. We propose that the ability of CNTF to enhance axon regeneration and protect peripheral nerve from structural and functional indices of diabetic peripheral neuropathy is associated with targeting of mitochondrial function, in part via NF-κB activation, and improvement of cellular bioenergetics.

  4. Single-cell NF-kappaB dynamics reveal digital activation and analogue information processing.

    Science.gov (United States)

    Tay, Savaş; Hughey, Jacob J; Lee, Timothy K; Lipniacki, Tomasz; Quake, Stephen R; Covert, Markus W

    2010-07-08

    Cells operate in dynamic environments using extraordinary communication capabilities that emerge from the interactions of genetic circuitry. The mammalian immune response is a striking example of the coordination of different cell types. Cell-to-cell communication is primarily mediated by signalling molecules that form spatiotemporal concentration gradients, requiring cells to respond to a wide range of signal intensities. Here we use high-throughput microfluidic cell culture and fluorescence microscopy, quantitative gene expression analysis and mathematical modelling to investigate how single mammalian cells respond to different concentrations of the signalling molecule tumour-necrosis factor (TNF)-alpha, and relay information to the gene expression programs by means of the transcription factor nuclear factor (NF)-kappaB. We measured NF-kappaB activity in thousands of live cells under TNF-alpha doses covering four orders of magnitude. We find, in contrast to population-level studies with bulk assays, that the activation is heterogeneous and is a digital process at the single-cell level with fewer cells responding at lower doses. Cells also encode a subtle set of analogue parameters to modulate the outcome; these parameters include NF-kappaB peak intensity, response time and number of oscillations. We developed a stochastic mathematical model that reproduces both the digital and analogue dynamics as well as most gene expression profiles at all measured conditions, constituting a broadly applicable model for TNF-alpha-induced NF-kappaB signalling in various types of cells. These results highlight the value of high-throughput quantitative measurements with single-cell resolution in understanding how biological systems operate.

  5. Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-{kappa}B signaling

    Energy Technology Data Exchange (ETDEWEB)

    Tsou, Tsui-Chun, E-mail: tctsou@nhri.org.tw [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan (China); Yeh, Szu-Ching; Tsai, Feng-Yuan; Lin, Ho-Jane [Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan (China); Cheng, Tsun-Jen [Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University, Taipei, Taiwan (China); Chao, How-Ran [Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan (China); Tai, Lin-Ai [Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County, Taiwan (China)

    2010-11-15

    This study investigated inflammatory effects of zinc oxide (ZnO) particles on vascular endothelial cells. The effects of 50 and 100-nm ZnO particles on human umbilical vein endothelial cells (HUVECs) were characterized by assaying cytotoxicity, cell proliferation, and glutathione levels. A marked drop in survival rate was observed when ZnO concentration was increased to 45 {mu}g/ml. ZnO concentrations of {<=}3 {mu}g/ml resulted in increased cell proliferation, while those of {<=}45 {mu}g/ml caused dose-dependent increases in oxidized glutathione levels. Treatments with ZnO concentrations {<=}45 {mu}g/ml were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) protein, an indicator of vascular endothelium inflammation, revealing that ZnO particles induced a dose-dependent increase in ICAM-1 expression and marked increases in NF-{kappa}B reporter activity. Overexpression of I{kappa}B{alpha} completely inhibited ZnO-induced ICAM-1 expression, suggesting NF-{kappa}B plays a pivotal role in regulation of ZnO-induced inflammation in HUVECs. Additionally, TNF-{alpha}, a typical inflammatory cytokine, induced ICAM-1 expression in an NF-{kappa}B-dependent manner, and ZnO synergistically enhanced TNF-{alpha}-induced ICAM-1 expression. Both 50 and 100-nm ZnO particles agglomerated to similar size distributions. This study reveals an important role for ZnO in modulating inflammatory responses of vascular endothelial cells via NF-{kappa}B signaling, which could have important implications for treatments of vascular disease.

  6. Communication - An Effective Tool for Implementing ISO 14001/EMS

    Energy Technology Data Exchange (ETDEWEB)

    Rachel Damewood; Bowen Huntsman

    2004-04-01

    The Idaho National Engineering and Environmental Laboratory (INEEL) received ISO 14001/EMS certification in June 2002. Communication played an effective role in implementing ISO 14001/EMS at the INEEL. This paper describes communication strategies used during the implementation and certification processes. The INEEL achieved Integrated Safety Management System (ISMS) and Voluntary Protection Program (VPP) Star status in 2001. ISMS implemented a formal process to plan and execute work. VPP facilitated worker involvement by establishing geographic units at various facilities with employee points of contact and management champions. The INEEL Environmental Management System (EMS) was developed to integrate the environmental functional area into its ISMS and VPP. Since the core functions of ISMS, VPP, and EMS are interchangeable, they were easy to integrate. Communication is essential to successfully implement an EMS. (According to ISO 14001 requirements, communication interacts with 12 other elements of the requirements.) We developed communication strategies that integrated ISMS, VPP, and EMS. For example, the ISMS, VPP, and EMS Web sites communicated messages to the work force, such as “VPP emphasizes the people side of doing business, ISMS emphasizes the system side of doing business, and EMS emphasizes the systems to protect the environment; but they all define work, identify and analyze hazards, and mitigate the hazards.” As a result of this integration, the work force supported and implemented the EMS. In addition, the INEEL established a cross-functional communication team to assist with implementing the EMS. The team included members from the Training and Communication organizations, VPP office, Pollution Prevention, Employee and Media Relations, a union representative, facility environmental support, and EMS staff. This crossfunctional team used various communication strategies to promote our EMS to all organization levels and successfully implemented EMS

  7. Arranging ISO 13606 archetypes into a knowledge base.

    Science.gov (United States)

    Kopanitsa, Georgy

    2014-01-01

    To enable the efficient reuse of standard based medical data we propose to develop a higher level information model that will complement the archetype model of ISO 13606. This model will make use of the relationships that are specified in UML to connect medical archetypes into a knowledge base within a repository. UML connectors were analyzed for their ability to be applied in the implementation of a higher level model that will establish relationships between archetypes. An information model was developed using XML Schema notation. The model allows linking different archetypes of one repository into a knowledge base. Presently it supports several relationships and will be advanced in future.

  8. ISO 9000 and/or Systems Engineering Capability Maturity Model?

    Science.gov (United States)

    Gholston, Sampson E.

    2002-01-01

    For businesses and organizations to remain competitive today they must have processes and systems in place that will allow them to first identify customer needs and then develop products/processes that will meet or exceed the customers needs and expectations. Customer needs, once identified, are normally stated as requirements. Designers can then develop products/processes that will meet these requirements. Several functions, such as quality management and systems engineering management are used to assist product development teams in the development process. Both functions exist in all organizations and both have a similar objective, which is to ensure that developed processes will meet customer requirements. Are efforts in these organizations being duplicated? Are both functions needed by organizations? What are the similarities and differences between the functions listed above? ISO 9000 is an international standard of goods and services. It sets broad requirements for the assurance of quality and for management's involvement. It requires organizations to document the processes and to follow these documented processes. ISO 9000 gives customers assurance that the suppliers have control of the process for product development. Systems engineering can broadly be defined as a discipline that seeks to ensure that all requirements for a system are satisfied throughout the life of the system by preserving their interrelationship. The key activities of systems engineering include requirements analysis, functional analysis/allocation, design synthesis and verification, and system analysis and control. The systems engineering process, when followed properly, will lead to higher quality products, lower cost products, and shorter development cycles. The System Engineering Capability Maturity Model (SE-CMM) will allow companies to measure their system engineering capability and continuously improve those capabilities. ISO 9000 and SE-CMM seem to have a similar objective, which

  9. ncISO Facilitating Metadata and Scientific Data Discovery

    Science.gov (United States)

    Neufeld, D.; Habermann, T.

    2011-12-01

    Increasing the usability and availability climate and oceanographic datasets for environmental research requires improved metadata and tools to rapidly locate and access relevant information for an area of interest. Because of the distributed nature of most environmental geospatial data, a common approach is to use catalog services that support queries on metadata harvested from remote map and data services. A key component to effectively using these catalog services is the availability of high quality metadata associated with the underlying data sets. In this presentation, we examine the use of ncISO, and Geoportal as open source tools that can be used to document and facilitate access to ocean and climate data available from Thematic Realtime Environmental Distributed Data Services (THREDDS) data services. Many atmospheric and oceanographic spatial data sets are stored in the Network Common Data Format (netCDF) and served through the Unidata THREDDS Data Server (TDS). NetCDF and THREDDS are becoming increasingly accepted in both the scientific and geographic research communities as demonstrated by the recent adoption of netCDF as an Open Geospatial Consortium (OGC) standard. One important source for ocean and atmospheric based data sets is NOAA's Unified Access Framework (UAF) which serves over 3000 gridded data sets from across NOAA and NOAA-affiliated partners. Due to the large number of datasets, browsing the data holdings to locate data is impractical. Working with Unidata, we have created a new service for the TDS called "ncISO", which allows automatic generation of ISO 19115-2 metadata from attributes and variables in TDS datasets. The ncISO metadata records can be harvested by catalog services such as ESSI-labs GI-Cat catalog service, and ESRI's Geoportal which supports query through a number of services, including OpenSearch and Catalog Services for the Web (CSW). ESRI's Geoportal Server provides a number of user friendly search capabilities for end users

  10. APPLICATION OF POWERBUILDER IN MACHINE MANUFACTURE AND ISO CERTIFICATION

    Institute of Scientific and Technical Information of China (English)

    Song Limei; Qu Xinghua; Ding Jinming; Ye Shenghua; Qian Xiaolong

    2004-01-01

    The manufacturing status has great effect on the product's quality.Now measuring data can be automatically sent to database in computer by interfaces.Based on the client/server design, management information system (MIS) of a company is set up by PowerBuilder and SQL Server.Statistic parameters can be automatically computed and control curves can be plotted on time, which can bring great convenience to the ISO quality certification.Searching and analyzing on the database system can give useful information to the administrator to improve the quality of products.

  11. Mouse Models of Hrs Nf2 Interaction

    Science.gov (United States)

    2008-01-01

    degeneration. These abnormalities, however, occurred in all genotypes. Only one malignant peripheral nerve sheath tumor ( MPNST ) was seen in 125...36 wt +/- N MPNST N Hyperostosis 43 wt +/- N N N N 45 wt +/- N N N N 52 wt +/- N N N N 60 wt +/- N N N N 62 wt +/- Calcifications Basal...to 18 months) and tissue decomposition MPNST : Malignant peripheral nerve sheath tumor W81XWH-05-1-0188 13 Table 2: Other Tumors Number HRS NF2

  12. Performance and fate of organics in a pilot MBR-NF for treating antibiotic production wastewater with recycling NF concentrate.

    Science.gov (United States)

    Wang, Jianxing; Li, Kun; Wei, Yuansong; Cheng, Yutao; Wei, Dongbin; Li, Mingyue

    2015-02-01

    A double membrane system comprising a membrane bioreactor (MBR) combined with a nanofiltration (NF) membrane was investigated on a pilot scale for the treatment of antibiotic production wastewater over a three-month period at a pharmaceutical company in Wuxi, China. By recycling the NF concentrate, the combined MBR-NF process was shown to be effective for the treatment of antibiotic production wastewater, resulting in excellent water quality and a high water yield of 92±5.6%. The water quality of the pilot-scale MBR-NF process was excellent; e.g., the concentrations of TOC, NH4(+)-N, TP were stable at 5.52, 0.68, 0.34 mg L(-1), respectively, and the values of turbidity and conductivity of the NF permeate were 0.15 NTU and 2.5 mS cm(-1), respectively; these values meet China's water quality standard requirements for industrial use (GB21903-2008). Not only were the antibiotic removal rates of spiramycin (SPM) and new spiramycin (NSPM) over 95%, the acute toxicity was also drastically reduced by the MBR-NF pilot system. The main organics in the MBR effluent were proteins, polysaccharides, and humic-like substances; they were almost completely retained by the NF membrane and further biodegraded in the MBR because the NF concentrate was recycled. The microbial community of the MBR did not significantly change with the recycling of the NF concentrate.

  13. Cadenas de rodillos. Tendencias de desarrollo y dimensionales según normas ISO.

    Directory of Open Access Journals (Sweden)

    M. E. García Domínguez

    2007-05-01

    Full Text Available El artículo es una compilación de resultados derivados de un estudio del desarrollo de la transmisión por cadena de rodillos que permite brindar información relevante sobre la tendencia del mercado internacional, innovaciones recientes en las cadenas de rodillos y las diferencias dimensionales que caracterizan las series geométricas de las cadenas ISO-A e ISO-B según la norma ISO 606:2004The paper is a compilation of some results associated with the development of roller chain transmissions and outstanding information in relations with the roller chain market trend, recent innovations and dimensional differences of ISO-A and ISO-B roller chain series according to ISO Standard 606:2004.

  14. 浅析ISO 9000与流程管理

    Institute of Scientific and Technical Information of China (English)

    刘立

    2007-01-01

    指出了ISO9000质量管理体系与业务流程管理(BPM)两者之间的关系,探讨了BPM是推动ISO9000持续改进的动力,列举了企业ISO9000与BPM结合的业务流程再造案例,说明组织在实施ISO9001标准,而未使用ISO9004指南标准时,流程管理是组织确保ISO9000质量管理体系得到持续改进的一种管理理论和方法.

  15. ISO 14000 information platform. Quarterly report, January 1--March 31, 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    GETE, through its ISO 14000 Information Platform, globeNet{trademark} (www.iso14000.net), seeks to provide FETC and the US Department of Energy (DOE) with a timely, accurate, and reliable information resource on the issue of environmental management standards. This resource allows FETC to keep track of the latest developments in the ISO 14000 arena, in DOE as well as throughout the federal complex and industry. Utilizing this information, FETC and DOE can make critical decisions on their own use and implementation of the ISO 14000 standards. The information platform also provides FETC with a forum to present information on its activities, such as the DOE ISO 14000 Pilot Projects, and as an information gathering and dissemination resource for its publications. GETE works with an array of national and international contacts and content sources to gather information and provide daily updates on ISO 14000 and related environmental management issues.

  16. ISO 9000 IMPLEMENTATION AND PERCEIVED ORGANISATIONAL OUTCOME: THE CASE OF A SERVICE ORGANISATION

    Directory of Open Access Journals (Sweden)

    Jasni Abdul Latif

    2003-01-01

    Full Text Available The study examines the relationship between the perceptions of the employees about ISO 9000 implementation and perceived organisational outcomes. A conceptual framework linking the level of employee influence and involvement, their perception of ISO 9000 and the perceived impact on their work and the organisational outcomes is posited. Data was collected from a random sample of 64 employees of a service organisation. Contrary to expectations, the work impact of ISO 9000 was not correlated with the efficiency and market outcomes of ISO 9000 certification but the employee involvement, influence and perception of ISO 9000 were. The conceptualization of the influence path of ISO 9000 implementation on the organisational outcomes may require rethinking as perception may have a direct effect on the outcomes without the mediation of the impact on work. In short, employees may report benefits or positive outcomes even though no change is reported in their work.

  17. Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

    Directory of Open Access Journals (Sweden)

    Andréia Cirolini

    2013-09-01

    Full Text Available The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the PetrifilmTM EB system and the TEMPO® EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.

  18. ISO 9001: 2008 Quality Assurance Assessment of Defense Acquisition University Processes

    Science.gov (United States)

    2012-09-27

    Report No. DoDIG-2012-143 September 27, 2012 ISO 9001 :2008 Quality Assurance Assessment of Defense Acquisition University...DATE 27 SEP 2012 2. REPORT TYPE 3. DATES COVERED 00-00-2012 to 00-00-2012 4. TITLE AND SUBTITLE ISO 9001 :2008 Quality Assurance Assessment of...ACQUISITION UNIVERSITY SUBJECT: ISO 9001 :2008 Quality Assurance Assessment of Defense Acquisition University Processes (Report No. DODIG-2012-143) The

  19. Implementace systému managementu kvality podle normy ISO 9001:2015

    OpenAIRE

    Čížková, Martina

    2015-01-01

    The main goal of my thesis is to show the process of transitioning the current quality management system (in accordance with the norm ISO 9001: 2008) into the new quality management system in accordance with the norm ISO 9001: 2015 in Sipral a.s. company. The thesis analyses current state in the company concerning requirements of the norm and suggests changes necessary for implementation of the new quality management system in accordance with the norm ISO 9001: 2015. The first section introdu...

  20. 贯彻ISO 9000标准的思路及想法

    Institute of Scientific and Technical Information of China (English)

    王天忻

    2003-01-01

    结合北京西站贯彻ISO 9000标准的经验,就贯彻ISO 9000标准的主要难点,以及针对难点应采取的对策,说明铁路基层运输企业贯彻ISO 9000标准并取得实效应注意的问题.

  1. 医院全面优质管理与ISO 9000

    Institute of Scientific and Technical Information of China (English)

    印梦筱; 温端改; 甘建和

    2002-01-01

    本文对医院全面优质管理与ISO 9000之间的联系、相互作用及在医院如何实施ISO 9000提出一些思考意见,希望对正欲实施ISO 9000族标准化管理的医院提供一点借鉴。

  2. ISO 9000族标准与全国质量管理奖

    Institute of Scientific and Technical Information of China (English)

    季坤

    2002-01-01

    @@ 2000版ISO 9000族核心标准均以八项质量管理原则为理论基础,采用以过程为基础的质量管理体系模式,提出了最基本的要求(ISO 9001:2000);提供达到优秀业绩的指南(ISO 9004:2000).

  3. [Approval of ISO/IEC 17025 and quality control of laboratory testing].

    Science.gov (United States)

    Yamamoto, Shigeki; Asakura, Hiroshi; Machii, Kenji; Igimi, Shizunobu

    2010-01-01

    First section of Division of Biomedical Food Research, National Institute of Health Sciences (NIHS) was approved by ISO/IEC 17025 as a laboratory having an appropriate laboratory testing technique. NIHS is the first national laboratory approved by ISO/IEC 17025. NIHS has also been accepted the appropriate technique and facility for the BSL3 level pathogens by ISO/IEC 17025. NIHS is necessary to take an external audit almost every year. This approval is renewed every 4 years.

  4. Interim Report on ISO TC 163 Working Group 3. Annual progress report

    Energy Technology Data Exchange (ETDEWEB)

    Fairey, Philip [Florida Solar Energy Center, Cocoa, FL (United States)

    2009-04-02

    This reports cover the initial year efforts of the International Standards Organization (ISO) to develop international standards for rating the energy performance of buildings. The author of this report is a participant in this effort. This report summarizes the activities of the ISO Working Group charged with development of these standards and makes recommendations to the sponsors for future U.S. involvement in this ISO effort.

  5. A Comparison of ISO 9001 and the Capability Maturity Model for Software,

    Science.gov (United States)

    1994-08-01

    intuitively correlated. The purpose of this report is to contrast the CMM and ISO 9001 , showing both their differences and their similarities. The results...of the analysis indicate that, although an ISO 9001 -compliant organization would not necessarily satisfy all of the level 2 key process areas, it...for a level 1 organization to receive 9001 station; similarly, there are areas addressed by ISO 9001 that are not addressed in the CMM. A level 3

  6. Microbead agglutination based assays

    KAUST Repository

    Kodzius, Rimantas

    2013-01-21

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

  7. Colorimetric protein assay techniques.

    Science.gov (United States)

    Sapan, C V; Lundblad, R L; Price, N C

    1999-04-01

    There has been an increase in the number of colorimetric assay techniques for the determination of protein concentration over the past 20 years. This has resulted in a perceived increase in sensitivity and accuracy with the advent of new techniques. The present review considers these advances with emphasis on the potential use of such technologies in the assay of biopharmaceuticals. The techniques reviewed include Coomassie Blue G-250 dye binding (the Bradford assay), the Lowry assay, the bicinchoninic acid assay and the biuret assay. It is shown that each assay has advantages and disadvantages relative to sensitivity, ease of performance, acceptance in the literature, accuracy and reproducibility/coefficient of variation/laboratory-to-laboratory variation. A comparison of the use of several assays with the same sample population is presented. It is suggested that the most critical issue in the use of a chromogenic protein assay for the characterization of a biopharmaceutical is the selection of a standard for the calibration of the assay; it is crucial that the standard be representative of the sample. If it is not possible to match the standard with the sample from the perspective of protein composition, then it is preferable to use an assay that is not sensitive to the composition of the protein such as a micro-Kjeldahl technique, quantitative amino acid analysis or the biuret assay. In a complex mixture it might be inappropriate to focus on a general method of protein determination and much more informative to use specific methods relating to the protein(s) of particular interest, using either specific assays or antibody-based methods. The key point is that whatever method is adopted as the 'gold standard' for a given protein, this method needs to be used routinely for calibration.

  8. Down-Regulation of Notchl and NF-κB by Curcumin in Breast Cancer Cells MDA-MB-231

    Institute of Scientific and Technical Information of China (English)

    LONG Li; CAO You-de

    2008-01-01

    Objective:To test whether the down-regulation of Notch1 gene expression by curcumin could inhibit cell growth and induce apoptosis,which may be associated mechanistically with the down-regulation of NF-κB in breast cancer cells. Methods:Breast cancer cell lines MDA-MB-231 were cultured in vitro and treated with different dosages of curcumin(0,1.25,5.0,20.0μmol/L)for dose-dependent assay and different time(0,24,48,72 h)at the dosage of 5.0μmol/L for time course assay.The changes of the mRNA and protein expression of Notch1 and NF-κB were measured by RT-PCR and Western Blot,and MTT assay was used to measure the change of proliferation. Results:The mRNA and protein levels of Notch 1 and NF-κB were decreased significantly in human breast cancer cell line with the increase of dosage of curcumin(P<0.05),and with the extension of time course(P<0.05).These changes suggested a dose- and time-dependent manner.The proliferation rate of cells also was significantly inhibited(P<0.05). Conclusion:The current results show that the Notch-1 signaling pathway is associated mechanistically with NF-κB activity during curcumin-induced cell growth inhibition and apoptosis of breast cancer cells.These results suggest that the down-regulation of Notch signaling by curcumin may be a novel strategy for the treatment of patients with breast cancer.

  9. Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-κB/bFGF signaling

    Institute of Scientific and Technical Information of China (English)

    Jun-Li Guo; Shao-Jiang Zheng; Yue-Nan Li; Wei Jie; Xin-Bao Hao; Tian-Fa Li; Li-Ping Xia

    2012-01-01

    AIM:To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line (SGC-7901) and determine the underlying molecular mechanism.METHODS:After SGC-7901 cells were treated with toxicarioside A at various concentrations (0.5,1.5,4.5,9.0 μg/mL) for 24 h or 48 h,cell viability was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay,and the motility and invasion of tumor cells were assessed by the Transwell chamber assay.Immunofluorescence staining,reverse transcription polymerase chain reaction and Western blotting were performed to detect the expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR1),and nuclear factorkappa B (NF-κB) activation was examined by electrophoretic mobility shift assay.RESULTS:The results showed that toxicarioside A was capable of reducing cell viability,inhibiting cell growth,and suppressing cell migration and invasion activities in a time-and dose-dependent manner in SGC-7901cells.Further analysis revealed that not only the expression of bFGF and its high-affinity receptor FGFR1 but also the NF-κB-DNA binding activity were effectively blocked by toxicarioside A in a dose-dependent manner compared with the control group (P < 0.05 or P < 0.01).Interestingly,application of the NF-κB specific inhibitor,pyrrolidinedithiocarbamate (PDTC),to SGC-7901 cells significantly potentized the toxicarioside A-induced down-regulation of bFGF compared with the control group (P < 0.05).CONCLUSION:These findings suggest that toxicarioside A has an anti-gastric cancer activity and this effect may be achieved partly through down-regulation of NF-κB and bFGF/FGFR1 signaling.

  10. Up-stream and Down-stream Events in the NF-kB Activation Cascade in Response to Sparsely and Densely Ionizing Radiation

    Science.gov (United States)

    Langen, Britta; Hellweg, Christine; Baumstark-Khan, Christa; Ruscher, Roland; Schmitz, Claudia; Arenz, Andrea; Lau, Patrick; Meier, Matthias M.; Testard, Isabelle; Reitz, Guenther

    Radiation is a potentially limiting factor for long term orbital and interplanetary missions. Long-term exposure to galactic cosmic rays may shorten the healthy life-span after return to Earth due to cancer induction. During the mission, a solar flare can be life threatening. For better risk estimation and development of appropriate countermeasures, the study of the cellular radiation response is necessary. As an antiapoptotic factor, if activated in human cells by exposure to components of cosmic rays, the transcription factor nuclear factor κB (NF- κB) could influence the cancer risk of astronauts exposed to cosmic radiation and improve cellular survival after exposure to high radiation doses. In previous studies using a screening assay for the detection of NF-κB-dependent gene induction (HEK-pNF-κB-d2EGFP/Neo cells), the activation of this transcription factor by heavy ions was shown (Radiat. Res. 164: 527- 530, 2005). In this work, the events upstream of NF-κB attachment to its promoter and enhancer binding sites, and downstream expression of target genes were analysed. It is supposed that the ATM kinase mediates the signal from damaged DNA in the nucleus to kinases in the cytoplasm, such as NF-κB essential modulator (NEMO). For liberation of NF-κB and its nuclear translocation, the inhibitor of NF-κB (IκB) has to be degraded in the proteasom. In order to evaluate a role of NEMO in the radiation response, the survival of murine embryonic fibroblasts expressing wildtype NEMO and lacking NEMO in response to ionizing radiation was analyzed. Lack of NEMO impairs survival after X-ray exposure. The inhibition of ATM by KU-55933 suppresses the X-ray and heavy ion (13C, 35 MeV/u, LET 70 keV/µm) induced activation of NF-κB dependent gene expression, indicating the central position of ATM in radiation induced NF-κB activation. Short-term incubation with the proteasome inhibitor MG-132 also blocks NF-κB activation by radiation. These results suggest a role of

  11. The Effect of NF-κB Signalling Pathway on Expression and Regulation of Nacrein in Pearl Oyster, Pinctada fucata.

    Directory of Open Access Journals (Sweden)

    Juan Sun

    Full Text Available Nacrein is the first identified and widely investigated molluscan matrix protein and is considered to play an important role in the shell formation of the pearl oyster, Pinctada fucata. Here, we investigate the effect of the NF-κB signalling pathway on Nacrein gene expression in P. fucata to elucidate the mechanisms involved in shell formation. Inhibition of NF-κB signalling decreased Nacrein promoter-dependent luciferase activity. However, co-transfection of the Nacrein promoter vector with Pf-IKK or Pf-Rel expression plasmids could enhance luciferase activity, thus proving NF-κB signalling could regulate the transcriptional activity of the Nacrein promoter. Gene silencing by RNA interference and subsequent observation of the inner surface of the nacreous layer of oyster shells by SEM, showed that suppression of the gene Pf-Rel lead to a partial inhibition of Nacrein expression, not only at the mRNA level but also at the protein level. The inner surface of the shells became abnormal. Electrophoretic mobility shift assays (EMSAs revealed that Pf-Rel could directly bind to the relative sites of the Nacrein promoter. These results confirm that an important component of the NF-κB signalling pathway, Pf-Rel, can directly bind the Nacrein promoter in P. fucata, and regulate its transcription and shell formation.

  12. Panduratin A, a Possible Inhibitor in Metastasized A549 Cells through Inhibition of NF-Kappa B Translocation and Chemoinvasion

    Directory of Open Access Journals (Sweden)

    Mohd. Rais Mustafa

    2013-07-01

    Full Text Available In the present study, we investigated the effects of panduratin A (PA, isolated from Boesenbergia rotunda, on apoptosis and chemoinvasion in A549 human non-small cell lung cancer cells. Activation of the executioner procaspase-3 by PA was found to be dose-dependent. Caspase-3 activity was significantly elevated at the 5 µg/mL level of PA treatment and progressed to a maximal level. However, no significant elevated level was detected on procaspase-8. These findings suggest that PA activated caspase-3 but not caspase-8. Numerous nuclei of PA treated A549 cells stained brightly by anti-cleaved PARP antibody through High Content Screening. This result further confirmed that PA induced apoptotic cell death was mediated through activation of caspase-3 and eventually led to PARP cleavage. Treatment of A549 cells with PA resulted in a strong inhibition of NF-κB activation, which was consistent with a decrease in nuclear levels of NF-κB/p65 and NF-κB/p50 and the elevation of p53 and p21. Besides that, we also showed that PA significantly inhibited the invasion of A549 cells in a dose-dependent manner through reducing the secretion of MMP-2 of A549 cells gelatin zymography assay. Our findings not only provide the effects of PA, but may also be important in the design of therapeutic protocols that involve targeting of either p53 or NF-κB.

  13. Inhibitory effect of the antidepressant imipramine on NF-κB-dependent CXCL1 expression in TNFα-exposed astrocytes.

    Science.gov (United States)

    Lee, Young Han; Kim, Se Hyun; Kim, Yeni; Lim, Yoongho; Ha, Kyooseob; Shin, Soon Young

    2012-04-01

    Neuroinflammation is associated with the pathophysiology of various neurodegenerative diseases. Emerging evidence indicates that imipramine, a tricyclic antidepressant commonly used in depressive disorders, exhibits neuroprotective activity partly through anti-inflammatory effects. However, the molecular mechanisms underlying imipramine-mediated anti-inflammatory response are poorly understood. In this study, rat primary cultured astrocytes were used to elucidate the effect of the imipramine on TNFα-induced inflammatory responses. The results clearly demonstrated that imipramine reduced TNFα-induced CXCL1 expression through suppression of NF-κB-dependent CXCL1 promoter activity in primary astrocytes. In addition, we found that imipramine suppressed TNFα-induced phosphorylation of inhibitor of κBα (IκBα) and p65/RelA nuclear factor-κB (NF-κB), as well as the nuclear translocation of p65/RelA in primary cultured astrocytes. Chemotaxis assay demonstrated that astrocyte-derived CXCL1 contributed to migration of BV2 microglial cells toward astrocytes. This response was significantly blocked by treatment of astrocytes with imipramine or NF-κB inhibitor BAY11-7082. This study indicates that the antidepressant imipramine inhibits TNFα-induced CXCL1 expression via down-regulation of NF-κB signaling pathway in astrocytes and suggests that imipramine has a potential as an anti-inflammatory drug.

  14. Chromatin remodeling protein SMAR1 regulates NF-κB dependent Interleukin-8 transcription in breast cancer.

    Science.gov (United States)

    Malonia, Sunil K; Yadav, Bhawna; Sinha, Surajit; Lazennec, Gwendel; Chattopadhyay, Samit

    2014-10-01

    Interleukin-8 (IL-8) is a pleiotropic chemokine involved in metastasis and angiogenesis of breast tumors. The expression of IL-8 is deregulated in metastatic breast carcinomas owing to aberrant NF-κB activity, which is known to positively regulate IL-8 transcription. Earlier, we have shown that tumor suppressor SMAR1 suppresses NF-κB transcriptional activity by modulating IκBα function. Here, we show that NF-κB target gene IL-8, is a direct transcriptional target of SMAR1. Using chromatin immunoprecipitation and reporter assays, we demonstrate that SMAR1 binds to IL-8 promoter MAR (matrix attachment region) and recruits HDAC1 dependent co-repressor complex. Further, we also show that SMAR1 antagonizes p300-mediated acetylation of RelA/p65, a post-translational modification indispensable for IL-8 transactivation. Thus, we decipher a new role of SMAR1 in NF-κB dependent transcriptional regulation of pro-angiogenic chemokine IL-8.

  15. Harpagoside suppresses lipopolysaccharide-induced iNOS and COX-2 expression through inhibition of NF-kappa B activation.

    Science.gov (United States)

    Huang, Tom Hsun-Wei; Tran, Van H; Duke, Rujee K; Tan, Sharon; Chrubasik, Sigrun; Roufogalis, Basil D; Duke, Colin C

    2006-03-08

    Preparations of Harpagophytum procumbens, known as devil's claw, are used as an adjunctive therapy for the treatment of pain and osteoarthritis. Pharmacological evaluations have proven the effectiveness of this herbal drug as an anti-inflammatory and analgesic agent. The present study has investigated the mechanism of action of harpagoside, one of the major components of Harpagophytum procumbens, using human HepG2 hepatocarcinoma and RAW 264.7 macrophage cell lines. Harpagoside inhibited lipopolysaccharide-induced mRNA levels and protein expression of cyclooxygenase-2 and inducible nitric oxide in HepG2 cells. These inhibitions appeared to correlate with the suppression of NF-kappaB activation by harpagoside, as pre-treating cells with harpagoside blocked the translocation of NF-kappaB into the nuclear compartments and degradation of the inhibitory subunit IkappaB-alpha. Furthermore, harpagoside dose-dependently inhibited LPS-stimulated NF-kappaB promoter activity in a gene reporter assay in RAW 264.7 cells, indicating that harpagoside interfered with the activation of gene transcription. These results suggest that the inhibition of the expression of cyclooxygenase-2 and inducible nitric oxide by harpagoside involves suppression of NF-kappaB activation, thereby inhibiting downstream inflammation and subsequent pain events.

  16. IMPLEMENTASI MANAJEMEN MUTU ISO 9001: 2000 PADA SMKN 1 KALASAN

    Directory of Open Access Journals (Sweden)

    Jamiatun Jamiatun

    2015-10-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan  implementasi manajemen mutu  ISO 9001: 2000 pada mutu kinerja sekolah yang meliputi komponen input, proses, dan produk serta partisipasi orang tua siswa dalam pelaksanaan program-program sekolah di SMKN 1 Kalasan. Penelitian ini merupakan penelitian studi kasus dengan subjek penelitian  seluruh guru, karyawan, siswa dan orang tua siswa SMKN 1 Kalasan. Sampel dalam penelitian ini adalah guru dan karyawan SMKN 1 Kalasan yang telah mengabdi kurang lebih tiga tahun yang terdiri atas 84 guru dan 29 karyawan, dan orang tua/wali dan siswa yang duduk di kelas XII saat penelitian berlangsung terdiri atas 175 orang tua dan 312 siswa. Teknik analisis data yang digunakan dalam penelitian adalah deskriptif. Data dikumpulkan melalui  kuesioner, wawancara, observasi, dan dokumentasi. Hasil penelitian ini menunjukkan penilaian guru, karyawan, orang tua dan siswa terhadap mutu kinerja komponen input di SMKN 1 Kalasan sangat baik (63,19%, komponen proses sudah baik (50,35%, komponen produk sangat baik (73,61% dan partisipasi orang tua sangat baik (61,14%. Berdasarkan hasil ini, peneliti menyarankan bagi pihak SMKN 1 Kalasan untuk tetap melakukan perbaikan dalam implementasi Sistem Manajemen Mutu ISO 9001: 2000 agar mutu kinerja sekolah dapat ditingkatkan.

  17. ISO26000:远虑与近忧

    Institute of Scientific and Technical Information of China (English)

    周莉; 李向华

    2011-01-01

    在社会责任发展过程中,特别是二十一世纪以来,全球不同地区、不同层面、不同形式的组织和团体不断增强和丰富对社会责任的认识,积极参与实践,共同推动了社会责任在全球的广泛发展.国际标准化组织(ISO)历时6年多,于2010年11月正式发布ISO 26000《社会责任指南》国际标准,这一标准的发布,是ISO将标准制定视角从传统的工程技术领域转向国际社会政治经济和伦理道德领域的里程碑性标准,体现了全球社会责任的共识,是社会责任发展史上的里程碑,也是社会责任发展的新起点.

  18. ISO 17025: practical benefits of implementing a quality system.

    Science.gov (United States)

    Honsa, Julie D; McIntyre, Deborah A

    2003-01-01

    As a laboratory certified to ISO 9001:2000 and accredited to ISO 17025, rtech laboratories has incorporated an overall system for technical and quality management, which results in benefits observed in daily laboratory practices. Technical requirements were updated to include the addition of formal personnel training plans and detailed records, method development and validation procedures, measurement of method uncertainty, and a defined equipment calibration and maintenance program. In addition, a stronger definition of the sample preparation process was documented to maintain consistency in sampling, and a more rigorous quality control monitoring program was implemented for chemistry and microbiology. Management quality improvements focused on document control to maintain consistent analytical processes, improved monitoring of supplier performance, a contract review process for documenting customer requirements, and a system for handling customer comments and complaints, with continuous improvement through corrective and preventive action procedures and audits. Quarterly management review of corrective actions, nonconforming testing, and proficiency testing aid in determining long-term trending. The practical benefits of these technical and management quality improvements are seen on a daily basis in the laboratory. Faster identification and resolution of issues regarding methods, personnel or equipment, improved customer satisfaction, meeting quality requirements of specialized customers, and overall increased laboratory business are all the result of implementing an effective quality system.

  19. ISO Observations of Starless Bok Globules: Usually No Embedded Stars

    Science.gov (United States)

    Clemens, D.; Byrne, A.; Yun, J.; Kane, B.

    1996-01-01

    We have used ISOCAM to search the cores of a sample of small Bok globules previously classified to be mostly starless based on analysis of IRAS data. The ISO observations at 6.75microns (LW2 filter) and 14.5microns (LW3 filter) were sufficiently deep to enable detection of any low-mass hydrogen burning star or young stellar object (YSO) embedded in these globules. Of the 20 Bok globules observed by ISOCAM to date, we have reduced the data for 14. Of these, 13 show no evidence for faint red (S(sub v)(LW3) greater than S(sub v)(LW2)) stars missed by IRAS. One (CB68) does show the first mid-infrared detection of the very cool IRAS source toward this cloud, and may be a Class I or 0 YSO. We conclude, based on these new ISO observations, that Bok globules which have no IRAS sources are in general bona fide starless molecular clouds.

  20. Using ISO 25040 standard for evaluating electronic health record systems.

    Science.gov (United States)

    Oliveira, Marília; Novaes, Magdala; Vasconcelos, Alexandre

    2013-01-01

    Quality of electronic health record systems (EHR-S) is one of the key points in the discussion about the safe use of this kind of system. It stimulates creation of technical standards and certifications in order to establish the minimum requirements expected for these systems. [1] In other side, EHR-S suppliers need to invest in evaluation of their products to provide systems according to these requirements. This work presents a proposal of use ISO 25040 standard, which focuses on the evaluation of software products, for define a model of evaluation of EHR-S in relation to Brazilian Certification for Electronic Health Record Systems - SBIS-CFM Certification. Proposal instantiates the process described in ISO 25040 standard using the set of requirements that is scope of the Brazilian certification. As first results, this research has produced an evaluation model and a scale for classify an EHR-S about its compliance level in relation to certification. This work in progress is part for the acquisition of the degree of master in Computer Science at the Federal University of Pernambuco.

  1. DuPont IsoTherming clean fuel technology

    Energy Technology Data Exchange (ETDEWEB)

    Levinski, E. [E.I. DuPont Co., Wilmington, DE (United States)

    2009-07-01

    This poster described a hydroprocessing technology that DuPont has acquired from Process Dynamics, Inc. The IsoTherming clean fuel technology significantly reduces sulphur in motor fuels. The technology provides petroleum refiners the solution for meeting ultra low sulphur diesel requirements, at much lower costs than conventional technologies. IsoTherming hydroprocessing operates in a kinetically limited mode, with no mass transfer limitation. Hydrogen is delivered to the reactor in the liquid phase as soluble hydrogen, allowing for much higher space velocities than conventional hydrotreating reactors. Treated diesel is recycled back to the inlet of the reactor, generating less heat and more hydrogen into the reactor. The process results in a more isothermal reactor operation that allows for better yields, fewer light ends and greater catalyst life. The technology reduces coking, because the process provides enough hydrogen in the solution when cracking reactions take place. As a result, the process yields longer catalyst life. Other advantages for refiners include lower total investment; reduced equipment delivery lead times; reduced maintenance and operating costs; and configuration flexibility. tabs., figs.

  2. IsoDAR - A Definitive Search for Sterile Neutrinos

    Science.gov (United States)

    Barletta, William

    2013-04-01

    The steady development of high power cyclotrons, mostly in industry, is making possible a definitive, highly cost effective approach to the search for sterile neutrinos. In the proposed IsoDAR experiment a 600 kW beam of protons from a 60 MeV, H2+ cyclotron will impinge on a lithium target to generate copious Li-8. The Li-8 then decays at rest to yield a powerful source of anti-neutrinos that can be located ˜20 m from a hydrogenous detector. In particular our collaboration has been designing the accelerator / target system to be consistent with installation in the Kamioka mine to use the Kamland detector to record inverse beta decay events. We show that this source / detector combination can reveal or exclude the global-fit allowed region at 5 sigma in four months and differentiate between 1 and 2 sterile neutrinos with a few years of continued running. Our studies also show that high power cyclotrons will provide the most cost effective source for such an experiment. In addition, the 60 MeV IsoDAR cyclotron would be an ideal injector for DAEdALUS, our approach to measuring CP violation in the neutrino sector with decay-at-rest experiment.

  3. Characterization and robust filtering of multifunctional surfaces using ISO standards

    Science.gov (United States)

    Friis, K. S.; Godi, A.; De Chiffre, L.

    2011-12-01

    Engineered surfaces containing lubrication pockets and directional surface texture can decrease wear and friction in sliding or rolling contacts. A new generation of multifunctional (MUFU) surfaces has been created by hard machining followed by robot-assisted polishing. The production method allows for a large degree of freedom in specifying surface topography defined by frequency, depth and volume of the lubricant retention valleys, as well as the amount of load bearing area and the surface roughness. The surfaces cannot readily be characterized by means of conventional roughness parameters due to the multi-process production method involved. A series of MUFU surfaces were characterized by using the ISO 13565 standard for stratified surfaces and it is shown that the standard in some cases is inadequate for the characterization of a MUFU surface. To improve the filtering of MUFU surfaces, the robust Gaussian regression filtering technique described in ISO 16610-31 is analyzed and discussed. By slight modifications it is shown how the robust Gaussian regression filter can be applied to remove the form and find a suitable reference surface for further characterization of the MUFU surfaces—even for surfaces with a moderate to small plateau region.

  4. C26 Cancer-Induced Muscle Wasting Is IKKβ-Dependent and NF-kappaB-Independent

    Science.gov (United States)

    Cornwell, Evangeline W.; Mirbod, Azadeh; Wu, Chia-Ling; Kandarian, Susan C.; Jackman, Robert W.

    2014-01-01

    Existing data suggest that NF-kappaB signaling is a key regulator of cancer-induced skeletal muscle wasting. However, identification of the components of this signaling pathway and of the NF-κB transcription factors that regulate wasting is far from complete. In muscles of C26 tumor bearing mice, overexpression of dominant negative (d.n.) IKKβ blocked muscle wasting by 69% and the IκBα-super repressor blocked wasting by 41%. In contrast, overexpression of d.n. IKKα or d.n. NIK did not block C26-induced wasting. Surprisingly, overexpression of d.n. p65 or d.n. c-Rel did not significantly affect muscle wasting. Genome-wide mRNA expression arrays showed upregulation of many genes previously implicated in muscle atrophy. To test if these upregulated genes were direct targets of NF-κB transcription factors, we compared genome-wide p65 binding to DNA in control and cachectic muscle using ChIP-sequencing. Bioinformatic analysis of ChIP-sequencing data from control and C26 muscles showed very little p65 binding to genes in cachexia and little to suggest that upregulated p65 binding influences the gene expression associated with muscle based cachexia. The p65 ChIP-seq data are consistent with our finding of no significant change in protein binding to an NF-κB oligonucleotide in a gel shift assay, no activation of a NF-κB-dependent reporter, and no effect of d.n.p65 overexpression in muscles of tumor bearing mice. Taken together, these data support the idea that although inhibition of IκBα, and particularly IKKβ, blocks cancer-induced wasting, the alternative NF-κB signaling pathway is not required. In addition, the downstream NF-κB transcription factors, p65 and c-Rel do not appear to regulate the transcriptional changes induced by the C26 tumor. These data are consistent with the growing body of literature showing that there are NF-κB-independent substrates of IKKβ and IκBα that regulate physiological processes. PMID:24489962

  5. C26 cancer-induced muscle wasting is IKKβ-dependent and NF-kappaB-independent.

    Directory of Open Access Journals (Sweden)

    Evangeline W Cornwell

    Full Text Available Existing data suggest that NF-kappaB signaling is a key regulator of cancer-induced skeletal muscle wasting. However, identification of the components of this signaling pathway and of the NF-κB transcription factors that regulate wasting is far from complete. In muscles of C26 tumor bearing mice, overexpression of dominant negative (d.n. IKKβ blocked muscle wasting by 69% and the IκBα-super repressor blocked wasting by 41%. In contrast, overexpression of d.n. IKKα or d.n. NIK did not block C26-induced wasting. Surprisingly, overexpression of d.n. p65 or d.n. c-Rel did not significantly affect muscle wasting. Genome-wide mRNA expression arrays showed upregulation of many genes previously implicated in muscle atrophy. To test if these upregulated genes were direct targets of NF-κB transcription factors, we compared genome-wide p65 binding to DNA in control and cachectic muscle using ChIP-sequencing. Bioinformatic analysis of ChIP-sequencing data from control and C26 muscles showed very little p65 binding to genes in cachexia and little to suggest that upregulated p65 binding influences the gene expression associated with muscle based cachexia. The p65 ChIP-seq data are consistent with our finding of no significant change in protein binding to an NF-κB oligonucleotide in a gel shift assay, no activation of a NF-κB-dependent reporter, and no effect of d.n.p65 overexpression in muscles of tumor bearing mice. Taken together, these data support the idea that although inhibition of IκBα, and particularly IKKβ, blocks cancer-induced wasting, the alternative NF-κB signaling pathway is not required. In addition, the downstream NF-κB transcription factors, p65 and c-Rel do not appear to regulate the transcriptional changes induced by the C26 tumor. These data are consistent with the growing body of literature showing that there are NF-κB-independent substrates of IKKβ and IκBα that regulate physiological processes.

  6. Global Impact Estimation of ISO 50001 Energy Management System for Industrial and Service Sectors

    Energy Technology Data Exchange (ETDEWEB)

    Aghajanzadeh, Arian [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Therkelsen, Peter L. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Rao, Prakash [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); McKane, Aimee T. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2016-08-01

    A methodology has been developed to determine the impacts of ISO 50001 Energy Management System (EnMS) at a region or country level. The impacts of ISO 50001 EnMS include energy, CO2 emissions, and cost savings. This internationally recognized and transparent methodology has been embodied in a user friendly Microsoft Excel® based tool called ISO 50001 Impact Estimator Tool (IET 50001). However, the tool inputs are critical in order to get accurate and defensible results. This report is intended to document the data sources used and assumptions made to calculate the global impact of ISO 50001 EnMS.

  7. 结合术语学习ISO 9001标准(一)

    Institute of Scientific and Technical Information of China (English)

    杨裕涛

    2003-01-01

    @@ 引言:"术语"是ISO 9001标准的一部分 ISO 9001:2000标准第三章明确指出,"本标准采用ISO 9000中的术语和定义";第二章也明确了ISO 9000:2000标准所包含的条文,"通过在本标准中引用而构成为本标准的条文".

  8. ISO-66, a novel inhibitor of macrophage migration, shows efficacy in melanoma and colon cancer models.

    Science.gov (United States)

    Ioannou, Kyriaki; Cheng, Kai Fan; Crichlow, Gregg V; Birmpilis, Anastasios I; Lolis, Elias J; Tsitsilonis, Ourania E; Al-Abed, Yousef

    2014-10-01

    Macrophage migration inhibitory factor (MIF) is a pleiotropic pro-inflammatory cytokine, which possesses a contributing role in cancer progression and metastasis and, thus, is now considered a promising anticancer drug target. Many MIF-inactivating strategies have proven successful in delaying cancer growth. Here, we report on the synthesis of ISO-66, a novel, highly stable, small-molecule MIF inhibitor, an analog of ISO-1 with improved characteristics. The MIF:ISO-66 co-crystal structure demonstrated that ISO-66 ligates the tautomerase active site of MIF, which has previously been shown to play an important role in its biological functions. In vitro, ISO-66 enhanced specific and non-specific anticancer immune responses, whereas prolonged administration of ISO-66 in mice with established syngeneic melanoma or colon cancer was non-toxic and resulted in a significant decrease in tumor burden. Subsequent ex vivo analysis of mouse splenocytes revealed that the observed decrease in tumor growth rates was likely mediated by the selective in vivo expansion of antitumor-reactive effector cells induced by ISO-66. Compared to other MIF-inactivating strategies employed in vivo, the anticancer activity of ISO-66 is demonstrated to be of equal or better efficacy. Our findings suggest that targeting MIF, via highly specific and stable compounds, such as ISO-66, may be effective for cancer treatment and stimulation of anticancer immune responses.

  9. The iso-structural viscosity, configurational entropy and fragility of oxide liquids

    DEFF Research Database (Denmark)

    Yue, Yuanzheng

    2009-01-01

    This paper describes how the fragility of a liquid is linked to the ratio between the energy barrier (Eeq) for the equilibrium viscous behavior and that (Eiso) for the non-equilibrium iso-structural viscous behavior. Using the concept of iso-structural viscosity, two functions describing the vari......This paper describes how the fragility of a liquid is linked to the ratio between the energy barrier (Eeq) for the equilibrium viscous behavior and that (Eiso) for the non-equilibrium iso-structural viscous behavior. Using the concept of iso-structural viscosity, two functions describing...

  10. 国际旅行卫生保健中心引入ISO/IEC17020和ISO/IEC17025质量管理体系运行关键点分析%The key points of quality management systems on ISO/IEC17020 and ISO/IEC17025 in international travel health care centers

    Institute of Scientific and Technical Information of China (English)

    史玲莉; 闫冀焕; 沈军; 董辉; 王慧兰; 崔鹰; 韩伟

    2012-01-01

    Objective To analysis the suitability,, necessity and the key points of quality management systems on ISO/ IEC17020 and ISO/IEC17025 in international travel health care centers. Methods The differences and similarities of the clausals of ISO/IEC 1702 quality management systems were analyzed. Results The systems were harmonization and interrelated, meanwhile, there were different emphases and complement each other. Conclusion It is important to ensure the international health care centers in all aspects of work, effectively promote the management of scientific and standardization.%目的 探讨国际标准ISO/IEC17020和ISO/IEC17025管理体系在国际旅行卫生保健中心应用的适宜性、必要性以及运行的关键点.方法 比较分析ISO/IEC17020和ISO/IEC17025管理体系条款的异同.结果 两个体系既协调统一,相互关联,又各有侧重,相互补充.结论 ISO/IEC17020和ISO/IEC17025管理体系同时运行,确保国际旅行卫生保健中心的各个工作环节都处于受控状态,有力促进了管理的科学化、正规化.

  11. The Handwriting Performance of Children with NF1

    Science.gov (United States)

    Gilboa, Yafit; Josman, Naomi; Fattal-Valevski, Aviva; Toledano-Alhadef, Hagit; Rosenblum, Sara

    2010-01-01

    The objective of this study was to analyze the process and product of handwriting among children with Neurofibromatosis Type 1 (NF1) in comparison to those of Typically Developing (TD) children. Children with NF1 are at risk for some cognitive deficits, a wide range of deficits in perceptual skills and, motor and visual-motor integration skills…

  12. NF-κB Signaling Pathway, Inflammation and Colorectal Cancer

    Institute of Scientific and Technical Information of China (English)

    Soly Wang; Zhanjie Liu; Lunshan Wang; Xiaoren Zhang

    2009-01-01

    There is growing evidence for a connection between inflammation and tumor development, and the nuclear factor kappa B (NF-κB), a proinflammatory transcription factor, is hypothesized to promote tumorigenesis. Although the genetic evidence for the hypothesis has been lacking, recent papers have lent credence to this hypothesis. It has been reported that constitutive NF-κB activation in inflammatory bowel diseases (IBDs) increases risk of colorectal cancer (CRC) in the patients with the number of years of active disease. NF-κB activation might induce cellular transformation, mediate cellular proliferation, prevent the elimination of pre-neoplastic and fully malignant cells by up-regulating the anti-apoptosis proteins. Furthermore, NF-κB may contribute to the progression of CRC by regulating the expression of diverse target genes that are involved in cell proliferation (Cyclin D1), angiogenesis (VEGF, IL-8, COX2), and metastasis (MMP9). These findings implicate NF-κB inhibition as an important therapeutic target in CRC. However, due to lack of knowledge about the specific roles of different NF-κB subunits in different stage of carcinogenesis, and compounds to block specific subunits of NF-κB family, it will be a long time before the coming of targeting NF-κB in CRC therapy. Cellular & Molecular Immunology. 2009;6(5):327-334.

  13. Advances in NF-κB Signaling Transduction and Transcription

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    The molecular mechanisms for NF-κB signaling transduction and transcription have been the most attractive subjects for both basic research and pharmaceutical industries due to its important roles in both physiological and pathogenesis, particularly the close association of dysregulated NF-κB with tumorgenesis and inflammation. Several novel intracellular molecular events that regulate NF-κB activity have been described recently, including the discovery of an alternative signaling pathway that appears inducing a specific subset genes involved in adoptive immune response. Multi-level and multi-dimensional regulation of NF-κB activity by phosphorylation and acetylation modifications have unveiled and became the hottest targets for potentially tissue specific molecular interventions. Another emerging mechanism for NF-κB-responsive gene's regulation where NF-κB participates the transcriptional regulation independent of its cognate regulatory binding site within the target gene's promoter but facilitating the transaction activity of other involved transcription factors,that implicated an novel transcriptional activities for NF-κB. Thus, the current review will focus on these recent progresses that have been made on NF-κB signaling transduction and transcription. Cellular & Molecular Immunology. 2004; 1(6):425-435.

  14. Advances in NF-κB Signaling Transduction and Transcription

    Institute of Scientific and Technical Information of China (English)

    Weihua Xiao

    2004-01-01

    The molecular mechanisms for NF-κB signaling transduction and transcription have been the most attractive subjects for both basic research and pharmaceutical industries due to its important roles in both physiological and pathogenesis, particularly the close association of dysregulated NF-κB with tumorgenesis and inflammation. Several novel intracellular molecular events that regulate NF-κB activity have been described recently, including the discovery of an alternative signaling pathway that appears inducing a specific subset genes involved in adoptive immune response. Multi-level and multi-dimensional regulation of NF-κB activity by phosphorylation and acetylation modifications have unveiled and became the hottest targets for potentially tissue specific molecular interventions. Another emerging mechanism for NF-κB-responsive gene's regulation where NF-κB participates the transcriptional regulation independent of its cognate regulatory binding site within the target gene's promoter but facilitating the transaction activity of other involved transcription factors,that implicated an novel transcriptional activities for NF-κB. Thus, the current review will focus on these recent progresses that have been made on NF-κB signaling transduction and transcription. Cellular & Molecular Immunology. 2004;1(6):425-435.

  15. Regulation of NF-κB by TNF family cytokines.

    Science.gov (United States)

    Hayden, Matthew S; Ghosh, Sankar

    2014-06-01

    The NF-κB family of inducible transcription factors is activated in response to a variety of stimuli. Amongst the best-characterized inducers of NF-κB are members of the TNF family of cytokines. Research on NF-κB and TNF have been tightly intertwined for more than 25 years. Perhaps the most compelling examples of the interconnectedness of NF-κB and the TNF have come from analysis of knock-out mice that are unable to activate NF-κB. Such mice die embryonically, however, deletion of TNF or TNFR1 can rescue the lethality thereby illustrating the important role of NF-κB as the key regulator of transcriptional responses to TNF. The physiological connections between NF-κB and TNF cytokines are numerous and best explored in articles focusing on a single TNF family member. Instead, in this review, we explore general mechanisms of TNF cytokine signaling, with a focus on the upstream signaling events leading to activation of the so-called canonical and noncanonical NF-κB pathways by TNFR1 and CD40, respectively.

  16. ISO, unique explorer of the invisible cool universe

    Science.gov (United States)

    1995-10-01

    As Roger Bonnet, director of ESA's science programme, explains: "The ISO launch will be the culmination of twelve years of intensive effort to build tile mast powerful and precise infrared space telescope to dote, We fully expect astronomers in Europe. and all over the wand, to be rewarded with unprecedented insights into the particularly rich and fertile sources of the universe hidden in the infrared." For the mission looks set to mark a new step forward for astronomy. To date, infrared astronomy has always been hampered by two major difficulties. The first is that infrared radiation is thermal, calorific. This means that any body which is not absolutely cold naturally emits this type of "heat" or "hidden" radiation, Consequently, at the corresponding wavelengths, all telescopes, detectors and the Earth's atmosphere appear to shine spontaneously, Infrared observation is therefore tantamount to attempting to observe the stars in broad daylight using a telescope a million times more luminous than the stars themselves. Moreover, the atmosphere is partly or totally opaque to infrared light. Hence the great attraction of conducting such observations from space, above the Earth's atmospheric layers. using a very low-temperature telescope. The first - and, so far, truly - Satellite actually to have done so is the Infrared Astronomical Satellite (IRAS) - a joint UK/Dutch/US mission - which surveyed and catalogued the sky far ten months in 1983. A corner of the curtain was thus lifted, The scene was set. Now, the task is to observe each character, each object. And here, infrared astronomy is hampered by a second major difficulty: the very detection of light. For while infrared radiation was discovered back in 1800 (in the solar spectrum). infrared-sensitive photoelectric detectors did not materialise until the Second World War, IRAS itself had to make do without the multi-dimensional set-ups that would have allowed it to record true images. It had to build up its images

  17. Guía para la aplicación de las normas ISO 9001:2015 e ISO 14001:2015 a las empresas constructoras

    OpenAIRE

    Wu Fu, Xinli

    2016-01-01

    Dado la publicación de las nuevas normativas de ISO 9001:2015 e ISO 14001:2015, la intención de este trabajo es elaborar guía de aplicación de ambas normativas. El presente consta de tres partes esenciales: 1) Primera: interpretación de los criterios de ISO 9001:2015 Sistema de gestión de calidad aplicando sus requisitos a empresas del sector de la construcción. Para poder acreditar la tercera lengua, esta parte está redactada en inglés 2) Segunda: interpretación de los crit...

  18. 企业实施ISO 9000族标准的实践%The Practice of Implementing the Standard ISO 9000 in Enterprises

    Institute of Scientific and Technical Information of China (English)

    刘自山

    2007-01-01

    @@ 企业实施ISO 9000标准存在的主要问题 1 实施目的存在偏差 部分企业实施ISO 9000质量管理体系并不是出自企业强化内部质量管理、增强顾客满意度的需要,主要是迫于市场的压力,这些企业推行ISO 9000的目的仅仅是为了得到一张证书.

  19. Energy management systems in the practice. ISO 50001. A guideline for companies and institutions; Energiemanagementsysteme in der Praxis. ISO 50001. Leitfaden fuer Unternehmen und Organisationen

    Energy Technology Data Exchange (ETDEWEB)

    Kohlenborn, Walter; Kabisch, Sibylle; Klein, Johanna; Richter, Ina; Schuermann, Silas

    2012-06-15

    The guideline under consideration is an updated version of the guideline to the regulation EN 16001 and amended to ISO 50001. This contribution supports institutes in the implementation of an energy management system according to ISO 50001. The differences and commonalities to the European environment management system EMAS as well as to the regulation ISO 14001 are described. EMAS certified companies fulfil regularly all conditions of an energy management system. A practised energy management system is an excellent platform for the implementation of EMAS.

  20. ISO/9000 Quality Management System and university management%ISO/9000质量管理基本概念与高校管理

    Institute of Scientific and Technical Information of China (English)

    龚国友

    2012-01-01

    高等院校引入ISO/9000族标准质量管理体系,将促进学校改进教育方法,树立以学生为关注焦点的教育理念,阐述了ISO/9000族标准质量管理体系中的基本观念,8项基本管理原则与高校管理,进而探索了ISO/9000质量管理标准与高校传统管理的结合.

  1. 切实运行ISO 9000族质量管理体系%To Run the ISO 9000 Quality Management System

    Institute of Scientific and Technical Information of China (English)

    赵泽龙; 王梅俏; 王学志

    2012-01-01

    To run a good fusion of total quality management idea of ISO 9000 quality management system is to seize the basic management work leading.%从局实际情况出发,分析了实行ISO 9000族质量管理体系的重要性,认为切实运行好融合全面质量管理理念的ISO 9000族质量管理体系就是抓住了基础管理工作的龙头.

  2. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K [Pleasanton, CA; Snyderman, Neal J [Berkeley, CA; Rowland, Mark S [Alamo, CA

    2012-05-15

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  3. DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-α-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-κB pathways in human gastric epithelial cells

    Institute of Scientific and Technical Information of China (English)

    Suck-Chei Choi; Kang-Min Lee; Won-Jung Lee; Jae-Sik Park; Chang-Yell Shin; Tae-Young Oh; Chang-Duk Jun; Eun-Ju Choi; Hyun-Mee Oh; SungGa Lee; Jeong-Kun Lee; Meung-Su Lee; Yong-Il Shin; Suck-Jun Choi; Jeong-Ryong Chae

    2006-01-01

    AIM: To investigate whether, or how, DA-9601, which is a new gastroprotective agent, inhibits TNF-α-induced inflammatory signals in gastric epithelial AGS cells. METHODS: Cell viability was determined by MTT assay. IL-8 and CCL20 promoter activities were determined by a luciferease reporter gene assay. NF-κB-dependent transcriptional activity was determined by I-κBα degradation, NF-κB p65 nuclear translocation and a luciferase activity assay. IL-8 and CCL20 gene expression and protein secretion were determined by RT-PCR and an enzymelinked immunosorbent assay (ELISA). Total and phosphorylated forms of mitogen-activated protein kinases (MAPKs) were determined by Western blot. RESULTS: Treatment of AGS cells with DA-9601 reduced TNF-α-induced IL-8 and CCL20 promoter activities, as well as their gene expression and protein release. TNF-α also induced NF-κB-dependent transcriptional activity in AGS cells. In contrast, in cells treated with DA-9601, TNF-α-induced NF-κB activity was significantly blocked. Although all three MAP kinase family members were phosphorylated in response to TNF-α, a selective inhibitor of p38 kinase SB203580 only could inhibit both NF κB-dependent transcriptional activity and IL-8 and CCL20 production, suggesting a potential link between p38 kinase and NF-κB-dependent pathways in AGS cells. Interestingly, DA-9601 also selectively inhibited p38 kinase phosphorylation induced by TNF-α.CONCLUSION: DA-9601 blocked TNF-α-mediated inflammatory signals by potentially modulating the p38 kinase pathway and/or a signal leading to NF-κB dependent pathways in gastric epithelial cells.

  4. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network.

    Directory of Open Access Journals (Sweden)

    Mala V Rao

    Full Text Available Newly synthesized neurofilaments or protofilaments are incorporated into a highly stable stationary cytoskeleton network as they are transported along axons. Although the heavily phosphorylated carboxyl-terminal tail domains of the heavy and medium neurofilament (NF subunits have been proposed to contribute to this process and particularly to stability of this structure, their function is still obscure. Here we show in NF-H/M tail deletion [NF-(H/M(tailΔ] mice that the deletion of both of these domains selectively lowers NF levels 3-6 fold along optic axons without altering either rates of subunit synthesis or the rate of slow axonal transport of NF. Pulse labeling studies carried out over 90 days revealed a significantly faster rate of disappearance of NF from the stationary NF network of optic axons in NF-(H/M(tailΔ mice. Faster NF disappearance was accompanied by elevated levels of NF-L proteolytic fragments in NF-(H/M(tailΔ axons. We conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons.

  5. BiBTeX styl pro ČSN ISO 690 a ČSN ISO 690-2

    OpenAIRE

    Pyšný, Radek

    2009-01-01

    Tato práce popisuje průběh vytváření bibliografického stylu pro nástroj BibTeX, pomocí něhož lze v LaTeXu sázet bibliografické citace v souladu s normou ČSN ISO 690. V úvodu práce je proveden rozbor ČSN ISO 690. Následuje popis spolupráce mezi systémem LaTeX a nástrojem BibTeX. Podstatná část práce je věnována popisu struktury souborů, které BibTeX potřebuje pro svou funkci, tj. bibliografická databáze a bibliografický styl. Závěr této práce je věnovaný popisu vlastností vytvořeného bibliogra...

  6. ISO TR 22335:2007 experimental comparison%国际标准ISO TR22335实验比对

    Institute of Scientific and Technical Information of China (English)

    姚文清

    2013-01-01

    为了制定“技术报告”类国际标准ISO TR22335,2005年2月ISO/TC201技术委员会委托美国国家标准学会负责召集工作小组,小组成员由中国、匈牙利、日本、英国、美国、瑞士、韩国、菲律宾、俄罗斯等9个国家组成,中国等3个国家的6个实验室参加了实验比对,其中清华大学分析中心扫描俄歇电子能谱仪测得的标准偏差值最接近实际值,测得结果为:利用单孔栅网对97 nm的热氧化二氧化硅片进行界面溅射测量,测量的孔内(IA)标准偏差值为0.06 min,孔外(OA)标准偏差值为0.03min;氧化层厚度测量的标准偏差值为0.4 nm.

  7. Somatic neurofibromatosis type 1 (NF1) inactivation events in cutaneous neurofibromas of a single NF1 patient.

    Science.gov (United States)

    Emmerich, Denise; Zemojtel, Tomasz; Hecht, Jochen; Krawitz, Peter; Spielmann, Malte; Kühnisch, Jirko; Kobus, Karolina; Osswald, Monika; Heinrich, Verena; Berlien, Peter; Müller, Ute; Mautner, Victor-F; Wimmer, Katharina; Robinson, Peter N; Vingron, Martin; Tinschert, Sigrid; Mundlos, Stefan; Kolanczyk, Mateusz

    2015-06-01

    Neurofibromatosis type 1 (NF1) (MIM#162200) is a relatively frequent genetic condition that predisposes to tumor formation. The main types of tumors occurring in NF1 patients are cutaneous and subcutaneous neurofibromas, plexiform neurofibromas, optic pathway gliomas, and malignant peripheral nerve sheath tumors. To search for somatic mutations in cutaneous (dermal) neurofibromas, whole-exome sequencing (WES) was performed on seven spatially separated tumors and two reference tissues (blood and unaffected skin) from a single NF1 patient. Validation of WES findings was done using routine Sanger sequencing or Sequenom IPlex SNP genotyping. Exome sequencing confirmed the existence of a known familial splice-site mutation NM_000267.3:c.3113+1G>A in exon 23 of NF1 gene (HGMD ID CS951480) in blood, unaffected skin, and all tumor samples. In five out of seven analyzed tumors, we additionally detected second-hit mutations in the NF1 gene. Four of them were novel and one was previously observed. Each mutation was distinct, demonstrating the independent origin of each tumor. Only in two of seven tumors we detected an additional somatic mutation that was not associated with NF1. Our study demonstrated that somatic mutations of NF1 are likely the main drivers of cutaneous tumor formation. The study provides evidence for the rareness of single base pair level alterations in the exomes of benign NF1 cutaneous tumors.

  8. Germline and somatic NF1 mutations in sporadic and NF1-associated malignant peripheral nerve sheath tumours

    NARCIS (Netherlands)

    Bottillo, Irene; Ahiquist, Terje; Brekke, Helge; Danielsen, Stine A.; van den Berg, Eva; Mertens, Fredrik; Lothe, Ragnhild A.; Dallapiccola, Bruno

    2009-01-01

    Malignant peripheral nerve sheath tumours (MPNSTs) are a malignancy occurring with increased frequency in patients with neurofibromatosis type 1 (NF1). In contrast to the well-known spectrum of germline NF1 mutations, the information on somatic mutations in MPNSTs is limited. In this study, we scree

  9. No correlation between NF1 mutation position and risk of optic pathway glioma in 77 unrelated NF1 patients.

    Science.gov (United States)

    Hutter, Sonja; Piro, Rosario M; Waszak, Sebastian M; Kehrer-Sawatzki, Hildegard; Friedrich, Reinhard E; Lassaletta, Alvaro; Witt, Olaf; Korbel, Jan O; Lichter, Peter; Schuhmann, Martin U; Pfister, Stefan M; Tabori, Uri; Mautner, Victor F; Jones, David T W

    2016-05-01

    Neurofibromatosis type 1 (NF1) is a common monogenic disorder whereby affected individuals are predisposed to developing CNS tumors, including optic pathway gliomas (OPGs, occurring in ~15 to 20 % of cases). So far, no definite genotype-phenotype correlation determining NF1 patients at risk for tumor formation has been described, although enrichment for mutations in the 5' region of the NF1 gene in OPG patients has been suggested. We used whole exome sequencing, targeted sequencing, and copy number analysis to screen 77 unrelated NF1 patients with (n = 41) or without (n = 36; age ≥10 years) optic pathway glioma for germline NF1 alterations. We identified germline NF1 mutations in 69 of 77 patients (90 %), but no genotype-phenotype correlation was observed. Our data using a larger patient cohort did not confirm the previously reported clustering of mutations in the 5' region of the NF1 gene in patients with OPG. Thus, NF1 mutation location should not currently be used as a clinical criterion to assess the risk of developing OPGs.

  10. miR-486 sustains NF-κB activity by disrupting multiple NF-κB-negative feedback loops

    Institute of Scientific and Technical Information of China (English)

    Libing Song; Chuyong Lin; Hui Gong; Chanjuan Wang; Liping Liu; Jueheng Wu; Sha Tao

    2013-01-01

    Deubiquitinases,such as CYLD,A20 and Cezanne,have emerged as important negative regulators that balance the strength and the duration of NF-κB signaling through feedback mechanisms.However,how these serial feedback loops are simultaneously disrupted in cancers,which commonly exhibit constitutively activated NF-κB,remains puzzling.Herein,we report that miR-486 directly suppresses NF-κB-negative regulators,CYLD and Cezanne,as well as multiple A20 activity regulators,including ITCH,TNIP-1,TNIP-2 and TNIP-3,resulting in promotion of ubiquitin conjugations in NF-κB signaling and sustained NF-κB activity.Furthermore,we demonstrate that upregulation of miR-486 promotes glioma aggressiveness both in vitro and in vivo through activation of NF-κB signaling pathway.Importantly,miR-486 levels in primary gliomas significantly correlate with NF-κB activation status.These findings uncover a novel mechanism for constitutive NF-κB activation in gliomas and support a functionally and clinically relevant epigenetic mechanism in cancer progression.

  11. Promoter competition assay for analyzing gene regulation in joint tissue engineering.

    Science.gov (United States)

    Sun, Hui Bin; Malacinski, George M; Yokota, Hiroki

    2002-08-01

    We describe a new biochemical technique, "promoter competition assay," for examining the role of cis-acting DNA elements in tissue cultures. Recent advances in tissue engineering permit the culture of a variety of cells. Many tissues are engineered, however, without an appropriate understanding of molecular machinery that regulates gene expression and cellular growth. For elucidating the role of cis-acting regulatory elements in cellular differentiation and growth, we developed the promoter competition assay. This assay uses a transient transfer into cells of double-stranded DNA fragments consisting of cis-acting regulatory elements. The transferred DNA fragments act as a competitor and titrate the function of their genomic counterparts. Using synovial cells derived from a rheumatoid arthritis patient, we examined a role of NF-kappa B binding sites in the regulation of the expression of matrix metalloproteinase (MMP) genes. The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13.

  12. ISO sees the pattern in the cosmic wallpaper

    Science.gov (United States)

    1998-07-01

    Two dozen distant galaxies that are undergoing a process of intense evolution --either merging to build larger ones or reaching their final shape-- have been detected by a team of French astronomers using the European Space Agency's ISO space telescope. These are the first individual objects known to contribute their energy to the bulk of the Cosmic Infrared Background, a radiation that fills the entire universe like a wallpaper, emitted at the era when galaxies were formed. The new-found distant galaxies are indeed like the 'pattern' in this 'cosmic wallpaper'. This discovery will for the first time enable scientists to test different theories of galaxy formation, and therefore to tackle a key problem of astronomy --the birth process of galaxies which has remained a mystery so far, mainly because current telescopes cannot reach that far back in time, about 12,000 million years ago. This obstacle has been partially overcome by the French team headed by Jean-Loup Puget, at the Institut d'Astrophysique Spatiale in Paris, precisely because they searched for the primeval galaxies by focusing first on the study of the cosmic wallpaper, the 'Cosmic Infrared Background' (see note to editors below). This background glow that fills the whole universe is a by-product of the galaxy formation itself, a relic of the era when the first galaxies were being born. Its existence was predicted three decades ago and it was known to be detectable only at infrared wavelengths, as the dust enshrouding young galaxies causes them to be both opaque in visible light but bright at infrared wavelengths. However, this cosmic wallpaper turned out to be very dim: only two years ago Puget's team detected it after a careful analysis of data from NASA's COBE satellite. Once the 'Cosmic Infrared Background' had been found, the next step was to disentangle it into the sources contributing to it, that is, into the young galaxies in evolution or the 'pattern' in the wallpaper. The two dozen distant

  13. ASEGURAMIENTO DE LA CALIDAD DE PRODUCTO MEDIANTE ISO 9000-2000

    Directory of Open Access Journals (Sweden)

    Jhonny Avilio Olivar Contreras

    2014-10-01

    Full Text Available This paper proposes as a tool for quality processes, organizational management through the implementation of ISO 9000-2000. Basically is to acquire knowledge and update on the proposal of this rule. Then, this paper aims General Objective: "To ensure the product quality in business management through ISO 9000-2000". As Specific objectives are listed as follows: 1 Describe what the ISO 9000-2000 Standard and compendium of rules derived or related 2 Identify the advantages and disadvantages of the implementation of a System of Quality Management Standards under ISO 9000-2000, 3 List the application requirements required by the standard. 4 Conceptualize Process Certification under ISO. 5 List the Eight Principles of Quality Management according to ISO standards. The methodology of the present work is framed in a model of Documentary Research that analyzes different aspects about ISO through a comprehensive and systematic investigation of documents related to that topic. The level of research is Exploratory; The fundamental idea is to update knowledge of ISO, which the researcher believes the country is little current information exists, more so because Venezuela withdrew its membership from the ISO Organization in 2006 Any organization business desired implantation and subsequent certification of product quality through ISO, you should do on their own, which increases the costs to the respective adjustments and productivity. This documentary provides exploratory proposal primarily a guide to the organization concerned in the design and implementation of a quality management under ISO and, secondly, to know the core principles thereof. The result of applying the improvements and to ensure product quality in a reputable company out organizational process, then to manage through this type of standard. Thus it generates added value to the product and the organization through continuous improvement.

  14. Asiaticoside attenuates lipopolysaccharide-induced acute lung injury via down-regulation of NF-κB signaling pathway.

    Science.gov (United States)

    Qiu, Jiaming; Yu, Lijun; Zhang, Xingxing; Wu, Qianchao; Wang, Di; Wang, Xiuzhi; Xia, Cheng; Feng, Haihua

    2015-05-01

    Asiaticoside (AS), a triterpene glycoside isolated from Centella asiatica, has been shown to possess potent anti-inflammatory activity. However, the detailed molecular mechanisms of AS on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in mice are scanty. The purpose of this study was to evaluate the effect of AS on LPS-induced mouse ALI via down-regulation of NF-κB signaling pathway. We investigated the efficacy of AS on cytokine levels induced by LPS in bronchoalveolar lavage fluid (BALF) and RAW 264.7 cells. The production of cytokine (TNF-α and IL-6) was measured by enzyme-linked immunosorbent assay (ELISA). The lung wet-to-dry weight ratios were measured in LPS-challenged mice, and lung histopathologic changes observed via paraffin section were assessed. To further study the mechanism of AS protective effects on ALI, the activation of NF-κB p65 subunit and the degradation of IκBα were tested by western blot assay. We found that AS treatment at 15, 30 or 45mg/kg dose-dependently attenuated LPS-induced pulmonary inflammation by reducing inflammatory infiltration, histopathological changes, descended cytokine production, and pulmonary edema initiated by LPS. Furthermore, our results suggested that AS suppressed inflammatory responses in LPS-induced ALI through inhibition of the phosphorylation of NF-κB p65 subunit and the degradation of its inhibitor IκBα, and might be a new preventive agent of ALI in the clinical setting.

  15. ISO26000社会责任国际标准与企业知识产权保护%ISO 26000 and Intellectual Property Protection

    Institute of Scientific and Technical Information of China (English)

    黄坤; 管竹笋; 于志宏

    2012-01-01

    2010年11月1日,国际标准化组织(International Organization for Standardization,简称ISO)在2瑞士日内瓦国际会议中心发布了第一个真正意义上的全球社会责任标准——ISO 26000.ISO 26000的发布,是全球社会责任发展的里程碑和新起点,一个旨在推进可持续发展的社会责任管理新时代已然到来.ISO 26000发布以来,引起全球的竞相关注,世界各国都想在社会责任发展的浪潮中抢得先机和占到优势.

  16. ISO14000与煤炭企业的可持续发展%ISO14000 AND SUSTAINABLE DEVELOPMENT OF COAL ENTERPRISE

    Institute of Scientific and Technical Information of China (English)

    田伟君

    2001-01-01

    Combining characters of coal enterprise, this paper discusses the important meaning carrying out ISO14000 environmental standard in coal enterprise and how to carry out and actalize ISO14000 .In addition,it brings forward solvents for existent problems,to realize sustainable development of coal enterprise.%本文结合煤炭企业的自身特点,论述了在煤炭企业开展 ISO14000环境标准的重大意义和如何开展和实施 ISO14000。此外,还就存在的问题提出了解决办法,以实现煤炭企业的可持续发展。

  17. ISO9000 Standards and Hospital Quality Management%ISO 9000族标准与医院质量管理

    Institute of Scientific and Technical Information of China (English)

    顾伟民

    2000-01-01

    ISO 9000族标准体系,作为质量管理、质量保证的国际标准,为医院质量管理提供了重要的标准化形式.通过对ISO 9000族概况的介绍,以及各质量保证要素与医院质量管理的关系的分析,意在帮助人们进一步了解ISO 9000族标准的内涵,提高对ISO 9000在医院质量管理标准化建设方面重要意义的认识.

  18. ISO/IEC 27001:2013概述与改版分析%ISO/IEC 27001:2013 Introduction and Revision Analysis

    Institute of Scientific and Technical Information of China (English)

    白云广; 谢宗晓

    2014-01-01

    The paper briefly introduces the ISO/IEC 27001: 2013, and summarized the main changes according to ISO/IEC 27001: 2005, which can be referenced by users who have already deployed Information Security Management System (ISMS).%本文简要介绍了ISO/IEC 27001:2013,并分别按正文和附录A与ISO/IEC 27001:2005进行了对比,总结了其主要的变化之处,可以作为已经部署信息安全管理体系(ISMS)的用户作为升级参考。

  19. [Significance and future prospects of the ISO15189 accreditation topics on systems of operation in preparation for accreditation].

    Science.gov (United States)

    Ohno, Hiroie

    2010-01-01

    ISO 15189 is a standard for systems of operation to provide medical laboratory services. It is essential that daily operations be performed in accordance with ISO 15189, but acquisition of accreditation under ISO 15189 should not be the goal. Furthermore, since ISO 15189 does not stipulate operational procedures, the optimal method for the individual laboratory should be used. After obtaining ISO 15189 accreditation, the laboratory only has to manage and perform daily operations in accordance with ISO 15189 on the basis of the quality management system that has been established, and the quality of service that the medical laboratory provides will improve accordingly.

  20. Selected Extracts of Chinese Herbal Medicines: Their Effect on NF-κB, PPARα and PPARγ and the Respective Bioactive Compounds

    Directory of Open Access Journals (Sweden)

    E. Rozema

    2012-01-01

    Full Text Available Chinese herbal medicinal (CHM extracts from fourteen plants were investigated in cell-based in vitro assays for their effect on nuclear factor κB (NF-κB, a key regulator of inflammation, as well as on peroxisome proliferator-activated receptors (PPARs being key regulators of genes involved in lipid and glucose metabolism. 43% of the investigated CHMs showed NF-κB inhibitory and 50% PPARα and PPARγ activating effects. Apolar extracts from cortex and flos of Albizia julibrissin Durazz. and processed rhizomes of Arisaema sp. and Pinellia ternata (Thunb. Breit. that effectively inhibited TNF-α-induced NF-κB activation and dose-dependently activated PPARα and PPARγ were further investigated. Bioassay-guided fractionation and analysis by GC-MS led to the identification of fatty acids as PPAR agonists, including linoleic and palmitic acid.

  1. Selected Extracts of Chinese Herbal Medicines: Their Effect on NF-κB, PPARα and PPARγ and the Respective Bioactive Compounds.

    Science.gov (United States)

    Rozema, E; Atanasov, A G; Fakhrudin, N; Singhuber, J; Namduang, U; Heiss, E H; Reznicek, G; Huck, C W; Bonn, G K; Dirsch, V M; Kopp, B

    2012-01-01

    Chinese herbal medicinal (CHM) extracts from fourteen plants were investigated in cell-based in vitro assays for their effect on nuclear factor κB (NF-κB), a key regulator of inflammation, as well as on peroxisome proliferator-activated receptors (PPARs) being key regulators of genes involved in lipid and glucose metabolism. 43% of the investigated CHMs showed NF-κB inhibitory and 50% PPARα and PPARγ activating effects. Apolar extracts from cortex and flos of Albizia julibrissin Durazz. and processed rhizomes of Arisaema sp. and Pinellia ternata (Thunb.) Breit. that effectively inhibited TNF-α-induced NF-κB activation and dose-dependently activated PPARα and PPARγ were further investigated. Bioassay-guided fractionation and analysis by GC-MS led to the identification of fatty acids as PPAR agonists, including linoleic and palmitic acid.

  2. Comparative Study on the TQM & ISO 9000 in Agile Competition%敏捷竞争环境中TQM与ISO9000标准比较研究

    Institute of Scientific and Technical Information of China (English)

    苏秦

    2001-01-01

    本文对全面质量管理TQM、ISO9000质量管理保证标准和敏捷竞争进行了深入的对比分析,探讨敏捷竞争、TQM以及ISO9000的融合机理,为敏捷竞争质量管理的实施提供思路和途径.

  3. Comparative Study on the TQM & ISO9000in Agile Competition%敏捷竞争环境中TQM与ISO9000标准比较研究

    Institute of Scientific and Technical Information of China (English)

    苏秦

    2001-01-01

    本文对全面质量管理TQM、ISO9000质量管理质量保证标准和敏捷竞争进行了深入的对比分析,探讨敏捷竞争、TQM以及ISO9000的融合机理,为敏捷竞争质量管理的实施提供思路和途径.

  4. 再说高职院校实施ISO 9000%Further Development and Implement of ISO 9000 in Senior Occupational College

    Institute of Scientific and Technical Information of China (English)

    崔凤岐

    2003-01-01

    @@ 目前在高职教育服务领域里推行ISO 9000族标准,可以提高教学质量已在高职教育研究范围内得到共识.但在多数高职院校的管理层,特别是最高管理层对实施ISO 9000标准的必要性认识还有待提高.

  5. The Perspective and Implementation of Complaint Handling in ISO13485:2016%ISO 13485:2016抱怨处理的解析和实施

    Institute of Scientific and Technical Information of China (English)

    汪淑梅

    2016-01-01

    本文介绍了 ISO13485新版标准中抱怨的主要变化点和新要求,并就抱怨处理过程进行了详细说明。%This document introduces the main change points and new requirements of ISO13485:2016, and describes the process of complaint handling.

  6. Management system quality of service radio physics and RR. ISO 9001; Sistema de gestion de la calidad de un servicio de radiofisica y P. R. segun ISO 9001

    Energy Technology Data Exchange (ETDEWEB)

    Gil Agudo, A.; Torres Donaire, J.; Jimenez, J. C.; Carrascosa Fernandez, C.; Arjona Gutierrez, J.

    2011-07-01

    One of the tool increasingly deployed for the optimization of the procedures is the process management system according to a Quality Management. Likewise, for specific areas, such as central services within large institutions such as the General Hospitals of the systems of quality management is the most widely used ISO 9001. We describe in this paper our experience in implementing the system ISO 9001 in our Department of Radio physics and Radiation Protection (SRFPR).

  7. GeNF - Experimental report 2006

    Energy Technology Data Exchange (ETDEWEB)

    Pranzas, P.K.; Schreyer, A.; Willumeit, R. (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. of Materials Research

    2007-07-01

    At the Geesthacht Neutron Facility GeNF about 212 experiments were performed in 2006 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guests and by the GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 71 contributions in the present annual experimental report for the year 2006. The contributions may contain one or also several combined experiments. During 2006 the GKSS research reactor FRG-1 achieved an operation time of 197 days at the full 5 MW reactor power providing a neutron flux of ca. 1.4 x 10{sup 14} thermal neutrons/cm{sup 2}s. The cold neutron source was available during the complete operation time. The focus of the in house R and D work at GeNF instruments was the characterisation of nanostructures in engineering materials, the analysis of stresses and textures in welds and technical structures at ARES-2, TEX-2, DCD and SANS-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at PNR, NeRo, POLDI and ROeDI. The thoroughly upgraded residual stress diffractomer ARES-2 went in full operation in spring 2006 as well as the new neutron tomography device at GENRA-3. The installation of modern experiment control hardware and software based on LabView was completed on all designated instruments. In the appendices I and II the experimental reports of REFSANS at FRM II are attached as well as of the GKSS outstation HARWI-II at DESY. Both instruments started full operation in 2006. (orig.)

  8. GeNF - experimental report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Schreyer, A.; Vollbrandt, J.; Willumeit, R. (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. for Materials Research

    2004-07-01

    At the Geesthacht Neutron Facility GeNF about 210 experiments were performed in 2003 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guest and by the GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 76 contributions in the present annual experimental report for the year 2003. The contributions may contain one or also several combined experiments. During 2003 the GKSS research reactor FRG-1 achieved an operation time of 252 days at the full 5 MW reactor power providing a neutron flux of ca. 1,4 x 10{sup 14} thermal neutrons / cm{sup 2} s. The cold neutron source was available during the complete operation time. The focus of the in house R and D work at GeNF instruments was the characterisation of metal alloys, the analysis of stresses in welds and technical structures at ARES, FSS, DCD and SANS-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at PNR and ROeDI. The reflectomer TOREMA was thoroughly upgraded to the instrument NeRo and now offers new measurement possibilities. In the appendices the progress of the project REFSANS at FRM-II is reported as well as the experimental activities of the newly installed GKSS outstation HARWI-II at DESY. (orig.)

  9. [ISO 15189, Up-To-Date Information and Prospective View].

    Science.gov (United States)

    Shimoda, Katsuji

    2016-02-01

    "Accreditation Activities for Medical Laboratories in Japan" Audits for transition to ISO 15189:2012 continue to progress. Besides the continual increase of accreditations for medical laboratory testing and pathological examinations, preparations for the addition of physiological testing to the scope of accreditation have finally been completed. As a part of the revision to Japan's Medical Service Act, the external evaluation of medical laboratories is now a requirement to approve clinical trial core hospitals. Accordingly, the importance of third-party accreditation in medical laboratory testing is attracting a growing level of attention. World Accreditation Day 2015 "Accreditation: Supporting the Delivery of Health and Social Care" JAB is being used to make every effort to contribute to this system in order to improve the quality of healthcare in Japan and the health of its citizens.

  10. An ISO and IUE Study of Planetary Nebula NGC 2440

    Science.gov (United States)

    Salas, J. Bernard; Pottasch, S. R.; Feibelman, W. A.; Wesselius, P. R.; Oegerle, William R. (Technical Monitor)

    2002-01-01

    The infrared and ultraviolet spectra of planetary nebula NGC 2440 is presented. The observations were made respectively by the Infrared Space Observatory (ISO) and International Ultraviolet Explorer (IUE) These data, in conjunction with published optical observations have been used to derive electron temperature and density. A trend of electron temperature with ionization potential is found. In particular the electron temperature increases from 11000 to 18000 K with increasing IBM. The electron density has a constant value of 4500/cu cm in agreement with previous determination. The chemical abundance has been derived for the following elements; helium, carbon, nitrogen, oxygen, neon, sulfur and argon. The ionization correction factor turns out to be very small (almost unnecessary) for all species except sulfur.

  11. PROPILVEN LABORATORY EVALUATION ACCORDING TO ISO 17025:2005

    Directory of Open Access Journals (Sweden)

    Peggy Fereira R.

    2013-07-01

    Full Text Available This research aims to evaluate the procedures and methods used in the laboratory Propilven accordance with the provisions of ISO 17025:2005. Propilven, a petrochemicals company in Venezuela and in his laboratory determined the quality of the manufactured product (polypropylene, at all stages, so that the application of this technical standard will increase the confidence level of the results. The research was descriptive, with a non-experimental design, field, using as a data collection method, the interview, survey, observation and review of documents and records. Among the results, the standard currently stands, is in the process of standardization in the laboratory, with a degree of implementation of 50%. Additionally, among the recommendations made, are a series of steps to establish a quality management system based on this standard, which apply not only to this company but for any others who wish to demonstrate their competence to carry out tests and / or calibrations.

  12. isoSTED nanoscopy with intrinsic beam alignment.

    Science.gov (United States)

    Curdt, Franziska; Herr, Simon J; Lutz, Tobias; Schmidt, Roman; Engelhardt, Johann; Sahl, Steffen J; Hell, Stefan W

    2015-11-30

    Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ only a single objective lens typically feature a trade-off between axial and lateral resolution. 4Pi arrangements, in which the sample is illuminated coherently through two opposing lenses, have proven their potential for rendering the resolution isotropic. However, instrument complexity due to a large number of alignment parameters has so far thwarted the dissemination of this approach. Here, we present a 4Pi-STED setup combination, also called isoSTED nanoscope, where the STED and excitation beams are intrinsically co-aligned. A highly robust and convenient 4Pi cavity allows easy handling without the need for readjustments during imaging experiments.

  13. ISO9126 BASED SOFTWARE QUALITY EVALUATION USING CHOQUET INTEGRAL

    Directory of Open Access Journals (Sweden)

    Abdelkareem M. Alashqar

    2015-01-01

    Full Text Available Evaluating software quality is an important and essential issue in the development process because it helps to deliver a competitive software product. A decision of selecting the best software based on quality attributes is a type of multi-criteria decision-making (MCDM processes where interactions among criteria should be considered. This paper presents and develops quantitative evaluations by considering interactions among criteria in the MCDM problems. The aggregator methods such as Arithmetic Mean (AM and Weighted Arithmetic Mean (WAM are introduced, described and compared to Choquet Integral (CI approach which is a type of fuzzy measure used as a new method for MCDM. The comparisons are shown by evaluating and ranking software alternatives based on six main quality attributes as identified by the ISO 9126-1 standard. The evaluation experiments depend on real data collected from case studies.

  14. Getting the Most Neutrinos out of IsoDAR

    CERN Document Server

    Ciuffoli, Emilio; Mohammed, Hosam; Zhao, Fengyi; Deliyergiyev, Maksym

    2016-01-01

    Several experimental collaborations worldwide intend to test sterile neutrino models by measuring the disappearance of antineutrinos produced via isotope decay at rest (IsoDAR). The most advanced of these proposals have very similar setups, in which a proton beam strikes a target yielding neutrons which are absorbed by a high isotopic purity 7Li converter, yielding 8Li whose resulting decay yields the antineutrinos. In this note, we use FLUKA and GEANT4 simulations to investigate three proposed modifications of this standard proposal. In the first, the 7Li is replaced with 7Li compounds including a deuterium moderator. In the second, a gap is placed between the target and the converter to reduce the neutron bounce-back. Finally, we consider cooling the converter with liquid nitrogen. We find that these modifications can increase the antineutrino yield by as much as 50 percent. The first also substantially reduces the quantity of high purity 7Li which is needed.

  15. Iso-geometric shape optimization of magnetic density separators

    DEFF Research Database (Denmark)

    Dang Manh, Nguyen; Evgrafov, Anton; Gravesen, Jens;

    2014-01-01

    Purpose The waste recycling industry increasingly relies on magnetic density separators. These devices generate an upward magnetic force in ferro-fluids allowing to separate the immersed particles according to their mass density. Recently, a new separator design has been proposed that significantly...... covers with B-splines and defines a cost functional that measures the non-uniformity of the magnetic field in an area above the poles. The authors apply an iso-geometric shape optimization procedure, which allows us to accurately represent, analyze and optimize the geometry using only a few design...... variables. The design problem is regularized by imposing constraints that enforce the convexity of the pole cover shapes and is solved by a non-linear optimization procedure. The paper validates the implementation of the algorithm using a simplified variant of the design problem with a known analytical...

  16. Disruption of NF-κB signaling by fluoxetine attenuates MGMT expression in glioma cells

    Directory of Open Access Journals (Sweden)

    Song T

    2015-08-01

    Full Text Available Tao Song,1 Hui Li,2 Zhiliang Tian,3 Chaojiu Xu,4 Jingfang Liu,1 Yong Guo1 1Department of Neurosurgery, Xiangya Hospital, Central South University, 2Department of Immunology and Microbiology, Medical School of Jishou University, 3Department of Neurosurgery, 4Department of Oncology, The Hospital of Xiangxi Autonomous Prefecture, Jishou, People’s Republic of China Background: Resistance to temozolomide (TMZ in glioma is modulated by the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT. This study aimed to examine the effects of fluoxetine (FLT on MGMT expression in glioma cells and to investigate its underlying mechanisms.Materials and methods: Expression of MGMT, GluR1, or IκB kinase β (IKKβ was attenuated using short hairpin RNA-mediated gene knockdown. The 3-(4,5-dimethylthiazol -2-yl-2,5-diphenyltetrazolium bromide (MTT assay was used to evaluate the growth inhibition induced by FLT or TMZ. Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL was conducted to detect apoptotic cells. Western blotting was conducted to analyze the protein expression of MGMT, IKKβ, and NF-κB/p65 following FLT treatment. The murine subcutaneous xenograft model was used to evaluate the combinational effect of TMZ and FLT.Results: FLT markedly reduced MGMT expression in glioma cells, which was independent of GluR1 receptor function. Further, FLT disrupted NF-κB/p65 signaling in glioma cells and consequently attenuated NF-κB/p65 activity in regulating MGMT expression. Importantly, FLT sensitized MGMT-expressing glioma cells to TMZ, as FLT enhanced TMZ’s ability to impair the in vitro tumorigenic potential and to induce apoptosis in glioma cells. Knockdown of MGMT or IKKβ expression abolished the synergistic effect of FLT with TMZ in glioma cells, which suggested that FLT might sensitize glioma cells to TMZ through down-regulation of MGMT expression. Consistently, TMZ combined with FLT markedly attenuated NF

  17. OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

    KAUST Repository

    Kim, SoonKap

    2015-11-05

    OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes ( Ehd1, Hd3a , and RFT1 ) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

  18. ISO 26000对我国有色金属工业的影响及对策%Influence of ISO 26000 on Chinese nonferrous metals industry

    Institute of Scientific and Technical Information of China (English)

    赵家生; 王藩; 张强; 刘新新; 李明怡; 王玉岐; 吴桐; 刘宏浩; 刘世佳

    2015-01-01

    2010年11月,国际标准化组织发布了ISO 26000社会责任指南《简称ISO 26000》.对我国有色金属工业的发展既是机遇也是挑战.一方面ISO 26000有助于推动我国有色金属工业产业结构升级,贯彻国家“走出去”的发展战略,促进我国有色金属工业可持续发展,同时也为我国有色金属工业的发展带来了挑战.本文首先对ISO 26000制定意义进行阐述,随后着重分析ISO 26000的实施对我国有色金属工业发展的影响,最后提出有色金属工业企业在社会责任领域应对ISO 26000的策略和建议.

  19. Health risk evaluation of whole-body vibration by ISO 2631-5 and ISO 2631-1 for operators of agricultural tractors and recreational vehicles.

    Science.gov (United States)

    Park, Min-Soo; Fukuda, Takabumi; Kim, Tae-Gu; Maeda, Setsuo

    2013-01-01

    This paper presents experimental research evaluation of the vibration exposure for the health risk prediction during vehicle operation. The vibration measurements were carried out on a recreational vehicle and two types of agricultural tractors. The vibration levels were measured for different surfaces and vehicle speed conditions. Based on the analysis of the results in the small agricultural tractor operated in the workplace (frameworks), Sed exceeded 0.80 MPa by ISO2631-5:2004, and Av exceeded 0.89 m/s(2) by ISO2631-1:1997. That means that operators driving small agricultural tractors more than 8 h a day have a high probability of adverse health effects. However, the exposure value for the recreational vehicle had Sed < 0.5 MPa by ISO2631-5:2004 and Av < 0.5 m/s(2) by ISO2631-1:1997 on highways and local roads. That means Recreational Vehicle operators driving more than 8 h a day, have a low probability of adverse health effects. Also, for the recreational vehicle, vibration was taken at different speeds (40-60 km/h, 80 km/h, 100-120 km/h). However, the speed change did not appear to affect the vibration dose variation while driving a vehicle on the highway and road. Finally, the health effect index of ISO2631-5:2004 are almost the same as assessment of health effect by ISO2631-1:1997.

  20. Exploring the integration of ERP and ISO / TS16949 international quality system%探究ERP与ISO/TS16949国际质量体系融台

    Institute of Scientific and Technical Information of China (English)

    王峰

    2015-01-01

    ISO/TS16949是汽车行业重要的国际规范,1SO/TS 16949适用于直接零部件制造商以及汽车整车厂,ISO/TS16949不仅十分关注质量系统保证能力以及产品质量,更对质量管理系统是否具有有效性十分关注,ISO/TS16949国际质量体系将顾客作为中心,坚持预防为主.ISO/TS16949将满足顾客需求作为前提和基础,进而建立起持续改进质量具有高效性的质量管理体系.很多大型企业均将ISO/TS16949作为质量管理和制造的标准.探究了ERP与ISO/TS16949国际质量体系的融合.

  1. MOLES3: Implementing an ISO standards driven data catalogue

    Directory of Open Access Journals (Sweden)

    Graham A Parton

    2015-02-01

    Full Text Available ISO19156 Observations and Measurements (O&M provides a standardised framework for organising information about the collection of information about the environment.  Here we describe the implementation of a specialisation of O&M for environmental data, the Metadata Objects for Linking Environmental Sciences (MOLES3.MOLES3 provides support for organising information about data, and for user navigation around data holdings. The implementation described here, “CEDA-MOLES”, also supports data management functions for the Centre for Environmental Data Archival, CEDA. The previous iteration of MOLES (MOLES2 saw active use over five years, being replaced by CEDA-MOLES in late 2014. During that period important lessons were learnt both about the information needed, as well as how to design and maintain the necessary information systems. In this paper we review the problems encountered in MOLES2; how and why CEDA-MOLES was developed and engineered; the migration of information holdings from MOLES2 to CEDA-MOLES; and, finally, provide an early assessment of MOLES3 (as implemented in CEDA-MOLES and its limitations.Key drivers for the MOLES3 development included the necessity for improved data provenance, for further structured information to support ISO19115 discovery metadata  export (for EU INSPIRE compliance, and to provide appropriate fixed landing pages for Digital Object Identifiers (DOIs in the presence of evolving datasets. Key lessons learned included the importance of minimising information structure in free text fields, and the necessity to support as much agility in the information infrastructure as possible without compromising on maintainability both by those using the systems internally and externally (e.g. citing in to the information infrastructure, and those responsible for the systems themselves. The migration itself needed to ensure continuity of service and traceability of archived assets.

  2. ISO 9001 certification for hospitals in Bulgaria: does it help service?

    Science.gov (United States)

    Stoimenova, Assena; Stoilova, Ani; Petrova, Guenka

    2014-03-04

    The aim of our study is to review the published literature on establishment and implementation of ISO 9001 QMS in European hospitals, to study the availability of International Organization for Standardization (ISO) quality management systems (QMS) in Bulgarian hospitals and to outline the main advantages of ISO implementation in the hospitals in Bulgaria. The information on availability of ISO QMS in the hospitals in Bulgaria was gathered via Bulgarian certification register, the registries of various quality associations, websites of hospitals and certification companies presented in Bulgaria. A total number of 312 hospitals in Bulgaria were screened for the availability of QMS certified against the ISO 9001 requirements. The experience of European hospitals that implemented QMS is positive and the used approaches to improve the processes and the demonstrated effects from ISO implementation are analysed by the researchers. Unlike other European Union member states, the establishment of quality management systems in Bulgaria is not compulsory. However, our study revealed that 14.42% of the hospitals in Bulgaria have implemented and have certified quality systems against the requirements of ISO 9001. Our study confirmed that a quality management system using the ISO 9001 standard is useful for the hospitals as it can help to increase the operational efficiencies, to reduce errors, improve patient safety and produce a more preventive approach instead of a reactive environment.

  3. A primer on the Petri Net Markup Language and ISO/IEC 15909-2

    DEFF Research Database (Denmark)

    Hillah, L. M.; Kindler, Ekkart; Kordon, F.

    2009-01-01

    Standard, defines a transfer format for high-level nets. The transfer format defined in Part 2 of ISO/IEC 15909 is (or is based on) the \\emph{Petri Net Markup Language} (PNML), which was originally introduced as an interchange format for different kinds of Petri nets. In ISO/IEC 15909-2, however...

  4. Humana looks to ISO registration to address quality improvement and customer satisfaction.

    Science.gov (United States)

    2003-01-01

    Seeking new ways to improve standardization of clinical operations and customer focus, Louisville, KY-based Humana, Inc. announced in November that it has become the first healthcare company to be registered in the U.S. under ISO 9001:2000, a quality management standard published by the International Organization for Standardization (ISO).

  5. HL7 template model and EN/ISO 13606 archetype object model - a comparison.

    Science.gov (United States)

    Bointner, Karl; Duftschmid, Georg

    2009-01-01

    HL7 Templates and EN/ISO 13606 Archetypes are essential components for a semantically interoperable exchange of electronic health record (EHR) data. In this article the underlying models from which Templates and Archetypes are instantiated, namely the HL7 Template Model and the EN/ISO 13606 Archetype Object Model will be compared to identify discrepancies and analogies.

  6. New ISO/TC 163 activities on energy performance of buildings

    NARCIS (Netherlands)

    Dijk, D. van; Spiekman, M.

    2007-01-01

    The International Organization for standardization (ISO), Technical Committee 163 "Thermal Performance and Energy Use in the Built Environment" (ISO/TC 163) has produced and will produce sets of standards by reference to which performance requirements can be expressed at various levels, from materia

  7. EPA STANDARDS NETWORK FACT SHEET: ISO 14000: INTERNATIONAL ENVIRONMENTAL MANAGEMENT STANDARDS

    Science.gov (United States)

    This flyer provides an overview of the ISO 14000 series of International standards, supplying a brief history, structure of the Technical Committee (TC) 207, structure of the U.S. Technical Advisory Group (TAG) to ISO TC-207, status of the Standards development as of June 1997, w...

  8. 77 FR 71585 - Brookfield Energy Marketing LP v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2012-12-03

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Brookfield Energy Marketing LP v. ISO New England Inc.; Notice of...) filed a formal complaint against ISO New England Inc. (Respondent) alleging that the...

  9. 75 FR 51996 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2010-08-24

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings August 16... Commission staff may attend the following Midwest ISO-related meetings: Advisory Committee (10 a.m.-3 p.m.... Paul Hotel, 350 Market St., St. Paul, MN) September 15 October 20 November 17 December 1 Midwest...

  10. Factors That Relate to the Time to ISO 9000 Registration in Education Institutions

    Science.gov (United States)

    Thonhauser, Theresa

    2008-01-01

    The implementation of the ISO 9000 international quality management system in education has become a debated trend. Building on the literature, this study did not attempt to end the debate over ISO 9000 in education but looked at the problem with a new perspective. The purpose of this study was to identify factors that relate to the time required…

  11. The European large area ISO survey - III. 90-mu m extragalactic source counts

    DEFF Research Database (Denmark)

    Efstathiou, A.; Oliver, S.; Rowan-Robinson, M.

    2000-01-01

    We present results and source counts at 90 mum extracted from the preliminary analysis of the European Large Area ISO Survey (ELAIS). The survey covered about 12 deg(2) of the sky in four main areas and was carried out with the ISOPHOT instrument onboard the Infrared Space Observatory (ISO...

  12. 78 FR 32384 - New England Power Generators Association v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2013-05-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission New England Power Generators Association v. ISO New England Inc.; Notice of...) filed a formal complaint against ISO New England Inc. (Respondent) alleging that certain newly...

  13. A Proposed Interpretation of the ISO 10015 and Implications for HRD Theory and Research

    Science.gov (United States)

    Jacobs, Ronald L.; Wang, Bryan

    2007-01-01

    While recent discussions of ISO 10015- Guidelines for Training have done much to promote the need for the standard, no interpretation of the standard has been presented that would guide its actual implementation. This paper proposes an interpretation of the ISO 10015 based on the specifications of the guideline and two other standards related to…

  14. A formal analysis of ISO/IEEE P11073-20601 standard of medical device communication

    NARCIS (Netherlands)

    Goga, Nicolae; Costache, Stefania; Moldoveanu, Florica

    2009-01-01

    This article presents the formal work done for the ISO/IEEE P11073-20601 Draft Standard for Health informatics - Personal health device communication - Application profile Optimized exchange protocol. ISO/IEEE 11073 family defines standards for device communication between agents (e.g. blood pressur

  15. Restructuring the ISO 140 series for the future: background and approach

    NARCIS (Netherlands)

    Rasmussen, B.; Gerretsen, E.

    2005-01-01

    ISO 140 is the basic series of standards in Building Acoustics and consists of several parts relating to laboratory and field measurements. The standards were originally prepared by ISO/TC43/SC2 WGs in the early sixties and since then updated and implemented as European standards through CEN/TC126.

  16. In vitro anticancer property of a novel thalidomide analogue through inhibition of NF-κB activation in HL-60 cells

    Institute of Scientific and Technical Information of China (English)

    Min LI; Wan SUN; Ya-ping YANG; Bo XU; Wen-yuan YI; Yan-xia MA; Zhong-jun LI; Jing-rong CUI

    2009-01-01

    Aim: To investigate the anticancer property and possible mechanism of action of a novel sugar-substituted thalidomide derivative (STA-35) on HL-60 cells in vitro. Methods: TNF-α-induced NF-κB activation was determined using a reporter gene assay. The MTT assay was used to mea-sure cytotoxicity of the compound. The appearance of apoptotic Sub-G1 cells was detected by flow cytometry analysis. PARP cleavage and protein expression of NF-κB p65 and its inhibitor IκB were viewed by Western blotting. Results: STA-35 (1-20 μmol/L) suppressed TNF-α-induced NF-κB activation in transfected cells (HEK293/pNiFty-SEAP) in a dose- (1-20 μmol/L) and time-dependent (0-48 h) manner. It was also shown that STA-35 exerted a dose-dependent inhibitory effect on HL-60 cell proliferation with an IC50 value of 9.05 μmol/L. In addition, STA-35 induced apoptosis in HL-60 cells, as indicated by the appearance of a Sub-G1 peak in the cell cycle distribution, as well as poly ADP-ribose polymerase (PARP) cleavage. Subsequently, both NF-κB p65 and its inhibitor IκB gradually accumulated in cytoplasmic extracts in a dose- and time-dependent manner, indicating the blockage of NF-κB translocation induced by TNF-α from the cytoplasm to the nucleus. Conclusion: A novel sugar-substituted thalidomide derivative, STA-35, is potent toward HL-60 cells in vitro and induces apoptosis by the suppression of NF-κB activation.

  17. Transcriptional activation of human CDCA8 gene regulated by transcription factor NF-Y in embryonic stem cells and cancer cells.

    Science.gov (United States)

    Dai, Can; Miao, Cong-Xiu; Xu, Xiao-Ming; Liu, Lv-Jun; Gu, Yi-Fan; Zhou, Di; Chen, Lian-Sheng; Lin, Ge; Lu, Guang-Xiu

    2015-09-11

    The cell division cycle associated 8 (CDCA8) gene plays an important role in mitosis. Overexpression of CDCA8 was reported in some human cancers and is required for cancer growth and progression. We found CDCA8 expression was also high in human ES cells (hESCs) but dropped significantly upon hESC differentiation. However, the regulation of CDCA8 expression has not yet been studied. Here, we characterized the CDCA8 promoter and identified its cis-elements and transcription factors. Three transcription start sites were identified. Reporter gene assays revealed that the CDCA8 promoter was activated in hESCs and cancer cell lines. The promoter drove the reporter expression specifically to pluripotent cells during early mouse embryo development and to tumor tissues in tumor-bearing mice. These results indicate that CDCA8 is transcriptionally activated in hESCs and cancer cells. Mechanistically, two key activation elements, bound by transcription factor NF-Y and CREB1, respectively, were identified in the CDCA8 basic promoter by mutation analyses and electrophoretic motility shift assays. NF-Y binding is positively correlated with promoter activities in different cell types. Interestingly, the NF-YA subunit, binding to the promoter, is primarily a short isoform in hESCs and a long isoform in cancer cells, indicating a different activation mechanism of the CDCA8 transcription between hESCs and cancer cells. Finally, enhanced CDCA8 promoter activities by NF-Y overexpression and reduced CDCA8 transcription by NF-Y knockdown further verified that NF-Y is a positive regulator of CDCA8 transcription. Our study unearths the molecular mechanisms underlying the activation of CDCA8 expression in hESCs and cancer cells, which provides a better understanding of its biological functions.

  18. β-Carotene and lutein inhibit hydrogen peroxide-induced activation of NF-κB and IL-8 expression in gastric epithelial AGS cells.

    Science.gov (United States)

    Kim, Youngha; Seo, Ji Hye; Kim, Hyeyoung

    2011-01-01

    Reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2)) are involved in the pathogenesis of gastric inflammation. Interleukin-8 (IL-8) is a potent mediator of the inflammatory response by activating and recruiting neutrophils to the site of infection. Oxidant-sensitive transcription factor NF-κB regulates the expression of IL-8 in the immune and inflammatory events. Carotenoids (carotenes and oxygenated carotenoids) show antioxidant and anti-inflammatory activities. Low intake of β-carotene leads to high risk of gastric cancer. Oxygenated carotenoid lutein inhibited NF-κB activation in experimental uveitis. The present study aims to investigate whether β-carotene and lutein inhibit H(2)O(2)-induced activation of NF-κB and expression of IL-8 in gastric epithelial AGS cells. The cells were treated with carotenoids 2 h prior to the treatment of H(2)O(2). mRNA expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real time RT-PCR analyses. IL-8 level in the medium was determined by enzyme-linked immunosorbent assay. NF-κB activation was assessed by electrophoretic mobility shift assay. ROS levels of the cells were detected by confocal microscopic analysis for fluorescent dichlorofluorescein. As a result, H(2)O(2 )induced the activation of NF-κB and expression of IL-8 in AGS cells time-dependently. β-Carotene and lutein showed inhibitory effects on H(2)O(2)-induced increase in intracellular ROS levels, activation of NF-κB, and IL-8 expression in AGS cells. In conclusion, supplementation of carotenoids such as β-carotene and lutein may be beneficial for the treatment of oxidative stress-mediated gastric inflammation.

  19. Bacterial endotoxin enhances colorectal cancer cell adhesion and invasion through TLR-4 and NF-kappaB-dependent activation of the urokinase plasminogen activator system.

    LENUS (Irish Health Repository)

    Killeen, S D

    2009-05-19

    Perioperative exposure to lipopolysaccharide (LPS) is associated with accelerated metastatic colorectal tumour growth. LPS directly affects cells through Toll-like receptor 4 (TLR-4) and the transcription factor NF-kappaB. The urokinase plasminogen activator (u-PA) system is intimately implicated in tumour cell extracellular matrix (ECM) interactions fundamental to tumour progression. Thus we sought to determine if LPS directly induces accelerated tumour cell ECM adhesion and invasion through activation of the u-PA system and to elucidate the cellular pathways involved. Human colorectal tumour cell lines were stimulated with LPS. u-PA concentration, u-PA activity, active u-PA, surface urokinase plasminogen activator receptor (u-PAR) and TLR-4 expression were assessed by ELISA, colorimetric assay, western blot analysis and flow cytometry respectively. In vitro tumour cell vitronectin adhesion and ECM invasion were analysed by vitronectin adhesion assay and ECM invasion chambers. u-PA and u-PAR function was inhibited with anti u-PA antibodies or the selective u-PA inhibitors amiloride or WXC-340, TLR-4 by TLR-4-blocking antibodies and NF-kappaB by the selective NF-kappaB inhibitor SN-50. LPS upregulates u-PA and u-PAR in a dose-dependent manner, enhancing in vitro tumour cell vitronectin adhesion and ECM invasion by >40% (P<0.01). These effects were ameliorated by u-PA and u-PAR inhibition. LPS activates NF-kappaB through TLR-4. TLR-4 and NF-kappaB inhibition ameliorated LPS-enhanced u-PA and u-PAR expression, tumour cell vitronectin adhesion and ECM invasion. LPS promotes tumour cell ECM adhesion and invasion through activation of the u-PA system in a TLR-4- and NF-kappaB-dependent manner.

  20. Point mutation in the NF2 gene of HEI-193 human schwannoma cells results in the expression of a merlin isoform with attenuated growth suppressive activity

    Energy Technology Data Exchange (ETDEWEB)

    Lepont, Pierig; Stickney, John T.; Foster, Lauren A.; Meng, Jin-Jun; Hennigan, Robert F. [Department of Cell and Cancer Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Ip, Wallace [Department of Cell and Cancer Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States)], E-mail: wallace.ip@uc.edu

    2008-01-01

    Neurofibromatosis type 2 (NF2) is a genetic disorder characterized by the formation of bilateral schwannomas of the eighth cranial nerve. Although the protein product of the NF2 gene (merlin) is a classical tumor suppressor, the mechanism by which merlin suppresses cell proliferation is not fully understood. The availability of isolated tumor cells would facilitate a better understanding of the molecular function of merlin, but primary schwannoma cells obtained from patients grow slowly and do not yield adequate numbers for biochemical analysis. In this study, we have examined the NF2 mutation in HEI-193 cells, an immortalized cell line derived from the schwannoma of an NF2 patient. Previous work showed that the NF2 mutation in HEI-193 cells causes a splicing defect in the NF2 transcript. We have confirmed this result and further identified the resultant protein product as an isoform of merlin previously designated as isoform 3. The level of isoform 3 proteins in HEI-193 cells is comparable to the levels of merlin isoforms 1 and 2 in normal human Schwann cells and several other immortalized cell lines. In contrast to many mutant forms of merlin, isoform 3 is as resistant to proteasomal degradation as isoforms 1 and 2 and can interact with each of these isoforms in vivo. Cell proliferation assays showed that, in NF2{sup -/-} mouse embryonic fibroblasts, exogenously expressed merlin isoform 3 does exhibit growth suppressive activity although it is significantly lower than that of identically expressed merlin isoform 1. These results indicate that, although HEI-193 cells have undetectable levels of merlin isoforms 1 and 2, they are, in fact, not a merlin-null model because they express the moderately active growth suppressive merlin isoform 3.