WorldWideScience

Sample records for assay nf iso

  1. [In-house PCR practical knowledge: a sufficient validation according to NF EN ISO 15189?].

    Science.gov (United States)

    Védy, Serge; Valois, Aude; Budzilawski, David; Perez, Pascale; Puyhardy, Jean-Michel

    2013-01-01

    As far as molecular biology is concerned, numerous are laboratories using in-house method. Used since many years, medical biologists have often a good practical knowledge about them. According to ISO 15189 accreditation, is this knowledge sufficient for the validation of these methods? We have asked this question ourselves about our in-house Leishmaniasis PCR.

  2. ISO 17025 validation of a next-generation sequencing assay for relationship testing

    DEFF Research Database (Denmark)

    Buchard, Anders; Kampmann, Marie-Louise; Poulsen, Lena

    2016-01-01

    The HID-Ion AmpliSeq™ Identity Panel is a next-generation sequencing assay with 90 autosomal and 34 Y-chromosome SNPs that are amplified in one PCR step and subsequently sequenced using the Ion Personal Genome Machine (Ion PGM™) System. This assay was validated for relationship testing in our ISO...... 17025 accredited laboratory in 2015. Here, the essential parts of the validation report submitted to the Danish Accreditation Fund are presented. A total of 100 unrelated Danes were typed in duplicates and the locus balance, heterozygote balance (Hb) and noise levels were analysed in detail. Two loci...

  3. [Standard NF EN ISO 15189: comparative analysis with GBEA and implementation of the new reference support].

    Science.gov (United States)

    Rogowski, Julien; Annaix, Véronique

    2010-01-01

    Within reflexions of the healthcare system in France, Ballereau's report published in 2008 suggests modifications of medical biology supports by quality. The new reference system will be ISO 15189 which provides requirements for competence and quality. It differs from GBEA (Guidelines to Good Execution of Analysis) on the requirement of a quality system management. We accomplished a comparative study of these two standards of reference to identify differences, therefore elements to be worked or supported for accreditation process.

  4. Quantitative Analysis of NF-κB Transactivation Specificity Using a Yeast-Based Functional Assay.

    Directory of Open Access Journals (Sweden)

    Vasundhara Sharma

    Full Text Available The NF-κB transcription factor family plays a central role in innate immunity and inflammation processes and is frequently dysregulated in cancer. We developed an NF-κB functional assay in yeast to investigate the following issues: transactivation specificity of NF-κB proteins acting as homodimers or heterodimers; correlation between transactivation capacity and in vitro DNA binding measurements; impact of co-expressed interacting proteins or of small molecule inhibitors on NF-κB-dependent transactivation. Full-length p65 and p50 cDNAs were cloned into centromeric expression vectors under inducible GAL1 promoter in order to vary their expression levels. Since p50 lacks a transactivation domain (TAD, a chimeric construct containing the TAD derived from p65 was also generated (p50TAD to address its binding and transactivation potential. The p50TAD and p65 had distinct transactivation specificities towards seventeen different κB response elements (κB-REs where single nucleotide changes could greatly impact transactivation. For four κB-REs, results in yeast were predictive of transactivation potential measured in the human MCF7 cell lines treated with the NF-κB activator TNFα. Transactivation results in yeast correlated only partially with in vitro measured DNA binding affinities, suggesting that features other than strength of interaction with naked DNA affect transactivation, although factors such as chromatin context are kept constant in our isogenic yeast assay. The small molecules BAY11-7082 and ethyl-pyruvate as well as expressed IkBα protein acted as NF-κB inhibitors in yeast, more strongly towards p65. Thus, the yeast-based system can recapitulate NF-κB features found in human cells, thereby providing opportunities to address various NF-κB functions, interactions and chemical modulators.

  5. ISO 17025 validation of a next-generation sequencing assay for relationship testing.

    Science.gov (United States)

    Buchard, Anders; Kampmann, Marie-Louise; Poulsen, Lena; Børsting, Claus; Morling, Niels

    2016-10-01

    The HID-Ion AmpliSeq™ Identity Panel is a next-generation sequencing assay with 90 autosomal and 34 Y-chromosome SNPs that are amplified in one PCR step and subsequently sequenced using the Ion Personal Genome Machine (Ion PGM™) System. This assay was validated for relationship testing in our ISO 17025 accredited laboratory in 2015. Here, the essential parts of the validation report submitted to the Danish Accreditation Fund are presented. A total of 100 unrelated Danes were typed in duplicates and the locus balance, heterozygote balance (Hb) and noise levels were analysed in detail. Two loci were disregarded for casework because genotyping was uncertain. Hb for rs7520386 was skewed and high levels of noise were observed in rs576261. Three general acceptance criteria for analysis of single-source samples were defined: (i) sequencing depth > 200 reads, (ii) noise level 0.3. A Python script named SNPonPGM was developed to assist the analyst by highlighting loci that do not fulfil the general acceptance criteria. Furthermore, SNPonPGM has functions that reduce the hands-on time of the reporting officer to a few minutes per case. Mixtures with DNA from two individuals in a 1:24 ratio were readily identified using the three criteria and the SNPonPGM script. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization.

    Science.gov (United States)

    Cotelle, Sylvie; Dhyèvre, Adrien; Muller, Serge; Chenon, Pascale; Manier, Nicolas; Pandard, Pascal; Echairi, Abdelwahad; Silvestre, Jérôme; Guiresse, Maritxu; Pinelli, Eric; Giorgetti, Lucia; Barbafieri, Meri; Silva, Valéria C; Engel, Fernanda; Radetski, Claudemir M

    2015-01-01

    The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, "Assessment of genotoxic effects on higher plants-Vicia faba micronucleus test," for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials). The aim of this interlaboratory study on the Vicia micronucleus assay was to investigate the robustness of this in vivo assay in terms of its applicability in different countries where each participant were asked to use their own seeds and reference soil, in agreement with the ISO 29200 standard. The ISO 29200 standard protocol was adopted for this study, and seven laboratories from three countries (France, Italy, and Brazil) participated in the study. Negative and positive controls were correctly evaluated by 100 % of the participants. In the solid-phase test, the micronucleus frequency (number of micronuclei/1,000 cells) varied from 0.0 to 1.8 for the negative control (i.e., Hoagland's solution) and from 5.8 to 85.7 for the positive control (i.e., maleic hydrazide), while these values varied from 0.0 to 1.7 for the negative control and from 14.3 to 97.7 for the positive control in the case of liquid-phase test. The variability in the data obtained does not adversely affect the robustness of the protocol assessed, on the condition that the methodology described in the standard ISO 29200 is strictly respected. Thus, the Vicia micronucleus test (ISO 29200) is appropriate for complementing prokaryotic or in vitro tests cited in legislation related to risk assessment of genotoxicity potential.

  7. Validation of carbapenemase and extended-spectrum β-lactamase multiplex endpoint PCR assays according to ISO 15189.

    Science.gov (United States)

    Bogaerts, P; Rezende de Castro, R; de Mendonça, R; Huang, T-D; Denis, O; Glupczynski, Y

    2013-07-01

    To validate and accredit a set of three multiplex endpoint PCR assays, targeting the most important carbapenemase and minor extended-spectrum β-lactamase (ESBL) resistance genes, according to the international ISO 15189 particular requirements for the quality and competence of medical laboratories. Specific primers targeting ESBLs and carbapenemases were collected from the literature or designed internally. The multiplex PCRs were validated for sensitivity, specificity, intra- and inter-run reproducibility and accuracy by means of external quality control (EQC) using a collection of 137 characterized and referenced isolates. For each multiplex PCR assay, the presence of an extraction control ruled out false-negative results due to PCR inhibition or extraction faults. Amplicons were separated by capillary electrophoresis (QIAxcel system, Qiagen). The protocols and validation files were reviewed in the setting of an external audit conducted by the Belgian organization for accreditation (BELAC). Sensitivity, specificity and reproducibility for each targeted gene were 100%. All isolates from the three EQC panels were correctly identified by each PCR assay (accuracy 100%). The validation files were controlled by BELAC, and the PCR protocols were accepted as accredited according to ISO 15189. Three home-made multiplex PCRs targeting the major carbapenemases and four minor class A ESBL genes encountered in Gram-negative bacteria were accredited according to the ISO 15189 standards. This validation scheme could provide a useful model for laboratories aiming to accredit their own protocols.

  8. Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.

    Science.gov (United States)

    Faivre, Lionel; Lecouflet, Lucie; Liu, Wang-Qing; Khadher, Isabelle; Lahaie, Camille; Vidal, Michel; Legouvello, Sabine; Beaumont, Jean-Louis; Bierling, Philippe; Rouard, Hélène; Birebent, Brigitte

    2015-01-01

    For the last 40 years, the technique of extracorporeal photopheresis (ECP) has constantly developed. Among irradiation systems, those called "off-line" allow the validation of the quality of the cell therapy product. The inhibition of the proliferation of lymphocytes after ultraviolet irradiation (UVA) is usually verified by the tritiated thymidine assay as in vitro proliferation assay. The document presented here describes the results obtained while performing the setting up of an alternative proliferation assay using flow cytometry according to ISO 15189:2007 Standard. Cells samples taken before and after UVA irradiation were labeled with CarboxyFluorescein Succinimidyl Ester (CFSE) and then cultured with phytohemagglutinin-A (PHA). After 3 days, an analysis of the CFSE staining was realized by flow cytometry. In order to validate the shift in the method used according to Standard, the following tests were performed: 1) comparison with the reference method, 2) robustness test, 3) reagents stability. Comparison method demonstrated that the sensitivity of the CFSE test is 100%, the specificity is 89%, and the concordance is almost complete. The CFSE test is robust regarding parameters like cell concentration or PHA concentration. PHA and CFSE are stable for 6 months and one year, respectively. Validation of this alternative test, according to the ISO 15189:2007 Standard, has demonstrated good concordance with reference method. The results of the robustness and stability of reagents are appropriate for its routine use. Thus, the benefits of alternative technique make it a wise choice for the quality control of ECP in a cell therapy laboratory. © 2014 Clinical Cytometry Society.

  9. Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels.

    Science.gov (United States)

    Pop, Bianca; Niculae, Alexandru-Ștefan; Pop, Tudor Lucian; Răchișan, Andreea Liana

    2017-12-01

    Autism spectrum disorder (ASD) represents a very large set of neurodevelopmental issues with diverse clinical outcomes. Various hypotheses have been put forth for the etiology of autism spectrum disorder, including issues pertaining to oxidative stress. In this study, we conducted measurements of serum 8-Iso-Prostaglanding F2 α (8-iso-PGF2α, which is the results of non-enzimatically mediated polyunsaturated fatty acid oxidation) in a population of individuals with autism and a control group of age and sex matched controls. A quantitative assay of Paraoxonase 1 (PON1) was conducted. Data regarding comorbidities, structural MRI scans, medication, intelligence quotient (IQ) and Childhood Autism Rating Scale scores (CARS) were also included in our study. Our results show that patients diagnosed with autism have higher levels of 8-iso-PGF2α than their neurotypical counterparts. Levels of this particular metabolite, however, do not correlate with quantitative serum levels of Paraoxonase 1, which has been shown to be altered in individuals with autism. Neither 8-iso-PGF2α nor quantitative levels of PON1 provide any meaningful correlation with clinical or neuroimaging data in this study group. Future research should focus on providing data regarding PON 1 phenotype, in addition to standard quantitative measurements, in relation to 8-iso-PGF2α as well as other clinical and structural brain findings.

  10. A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins.

    Science.gov (United States)

    Bergamo, Elisa; Diani, Erica; Bertazzoni, Umberto; Romanelli, Maria Grazia

    2017-01-01

    HTLV-1 and HTLV-2 viruses express Tax transactivator proteins required for viral genome transcription and capable of transforming cells in vivo and in vitro. Although Tax oncogenic potential needs to be further elucidated, it is well established that Tax proteins activate, among others, transcription factors of the NF-ĸB family, which are involved in immune and inflammatory responses, cell growth, apoptosis, stress responses and oncogenesis. Here, we describe a reporter gene assay applied for quantitative analysis of Tax-dependent NF-ĸB activation. The procedure is based on co-transfection of two individual vectors containing the cDNA for firefly and Renilla luciferase enzymes and vectors expressing Tax proteins. The luciferase expression is driven by cis-NF-ĸB promoter regulatory elements responsive to Tax transactivating factor. This assay is particularly useful to investigate Tax influence on NF-ĸB activation mediated by viral or host factors.

  11. Validation of a single nucleotide polymorphism (SNP) typing assay with 49 SNPs for forensic genetic testing in a laboratory accredited according to the ISO 17025 standard

    DEFF Research Database (Denmark)

    Børsting, Claus; Rockenbauer, Eszter; Morling, Niels

    2009-01-01

    A multiplex assay with 49 autosomal single nucleotide polymorphisms (SNPs) developed for human identification was validated for forensic genetic casework and accredited according to the ISO 17025 standard. The multiplex assay was based on the SNPforID 52plex SNP assay [J.J. Sanchez, C. Phillips, C...... amplified in one PCR reaction. The SNPs were analysed by single base extension (SBE) and capillary electrophoresis. Twenty-three of the original SBE primers were altered to improve the overall robustness of the assay and to simplify the analysis of the SBE results. A total of 216 samples from 50 paternity...... cases and 33 twin cases were typed at least twice for the 49 SNPs. All electropherograms were analysed independently by two expert analysts prior to approval. Based on these results, detailed guidelines for analysis of the SBE products were developed. With these guidelines, the peak height ratio...

  12. A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer.

    Science.gov (United States)

    Altevogt, Dominik; Hrenn, Andrea; Kern, Claudia; Clima, Lilia; Bannwarth, Willi; Merfort, Irmgard

    2009-10-07

    Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

  13. Validation of a single nucleotide polymorphism (SNP) typing assay with 49 SNPs for forensic genetic testing in a laboratory accredited according to the ISO 17025 standard.

    Science.gov (United States)

    Børsting, Claus; Rockenbauer, Eszter; Morling, Niels

    2009-12-01

    A multiplex assay with 49 autosomal single nucleotide polymorphisms (SNPs) developed for human identification was validated for forensic genetic casework and accredited according to the ISO 17025 standard. The multiplex assay was based on the SNPforID 52plex SNP assay [J.J. Sanchez, C. Phillips, C. Børsting, K. Balogh, M. Bogus, M. Fondevila, C.D. Harrison, E. Musgrave-Brown, A. Salas, D. Syndercombe-Court, P.M. Schneider, A. Carracedo, N. Morling, A multiplex assay with 52 single nucleotide polymorphisms for human identification, Electrophoresis 27 (2006) 1713-1724], where 52 fragments were amplified in one PCR reaction. The SNPs were analysed by single base extension (SBE) and capillary electrophoresis. Twenty-three of the original SBE primers were altered to improve the overall robustness of the assay and to simplify the analysis of the SBE results. A total of 216 samples from 50 paternity cases and 33 twin cases were typed at least twice for the 49 SNPs. All electropherograms were analysed independently by two expert analysts prior to approval. Based on these results, detailed guidelines for analysis of the SBE products were developed. With these guidelines, the peak height ratio of a heterozygous allele call or the signal to noise ratio of a homozygous allele call is compared with previously obtained ratios. A laboratory protocol for analysis of SBE products was developed where allele calls with unusual ratios were highlighted to facilitate the analysis of difficult allele calls. The guidelines for allele calling proved to be highly efficient for the detection of DNA mixtures and contaminated DNA preparations. DNA from two individuals was mixed in seven different ratios ranging from 1:1 to 1:10; all mixtures were easily identified as mixtures.

  14. α-Iso-cubebenol inhibits inflammation-mediated neurotoxicity and amyloid beta 1-42 fibril-induced microglial activation.

    Science.gov (United States)

    Park, Sun Young; Park, Tae Gyeong; Lee, Sang-Joon; Bae, Yoe-Sik; Ko, Min J; Choi, Young-Whan

    2014-01-01

    To examine the antineuroinflammatory and neuroprotective activity of α-iso-cubebenol and its molecular mechanism of action in amyloid β (Aβ) 1-42 fibril-stimulated microglia. Aβ 1-42 fibrils were used to induce a neuroinflammatory response in murine primary microglia and BV-2 murine microglia cell lines. Cell viability was monitored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, protein expression and phosphorylation were determined by Western blot analysis, and matrix metalloproteinase-9 (MMP-9) activity was determined by gelatin zymography assay. In addition, prostaglandin E2 (PGE2), pro-inflammatory cytokines and chemokines were measured by ELISA, and the transactivity of nuclear factor (NF)-κB was determined by a reporter assay. α-Iso-cubebenol significantly inhibited Aβ 1-42 fibril-induced MMP-9, inducible nitric oxide synthase and cyclooxygenase-2 expressions and activity, without affecting cell viability. α-Iso-cubebenol also suppressed the production of tumour necrosis factor-α, IL-1β, IL-6, monocyte chemoattractant protein-1 and reactive oxygen species in a dose-dependent manner, while decreasing the nuclear translocation and transactivity of NF-κB by inhibiting the phosphorylation and degradation of the inhibitor of κB (IκB)α. α-Iso-cubebenol suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) in Aβ 1-42 fibril-stimulated microglia. Primary cortical neurons were protected by the inhibitory effect of α-iso-cubebenol on Aβ 1-42 fibril-induced neuroinflammatory response. α-Iso-cubebenol suppresses Aβ 1-42 fibril-induced neuroinflammatory molecules in primary microglia via the suppression of NF-κB/inhibitor of κBα and MAPK. Importantly, the antineuroinflammatory potential of α-iso-cubebenol is critical for neuroprotection. © 2013 Royal Pharmaceutical Society.

  15. Integration of Affinity Selection-Mass Spectrometry and Functional Cell-Based Assays to Rapidly Triage Druggable Target Space within the NF-κB Pathway.

    Science.gov (United States)

    Kutilek, Victoria D; Andrews, Christine L; Richards, Matthew P; Xu, Zangwei; Sun, Tianxiao; Chen, Yiping; Hashke, Andrew; Smotrov, Nadya; Fernandez, Rafael; Nickbarg, Elliott B; Chamberlin, Chad; Sauvagnat, Berengere; Curran, Patrick J; Boinay, Ryan; Saradjian, Peter; Allen, Samantha J; Byrne, Noel; Elsen, Nathaniel L; Ford, Rachael E; Hall, Dawn L; Kornienko, Maria; Rickert, Keith W; Sharma, Sujata; Shipman, Jennifer M; Lumb, Kevin J; Coleman, Kevin; Dandliker, Peter J; Kariv, Ilona; Beutel, Bruce

    2016-07-01

    The primary objective of early drug discovery is to associate druggable target space with a desired phenotype. The inability to efficiently associate these often leads to failure early in the drug discovery process. In this proof-of-concept study, the most tractable starting points for drug discovery within the NF-κB pathway model system were identified by integrating affinity selection-mass spectrometry (AS-MS) with functional cellular assays. The AS-MS platform Automated Ligand Identification System (ALIS) was used to rapidly screen 15 NF-κB proteins in parallel against large-compound libraries. ALIS identified 382 target-selective compounds binding to 14 of the 15 proteins. Without any chemical optimization, 22 of the 382 target-selective compounds exhibited a cellular phenotype consistent with the respective target associated in ALIS. Further studies on structurally related compounds distinguished two chemical series that exhibited a preliminary structure-activity relationship and confirmed target-driven cellular activity to NF-κB1/p105 and TRAF5, respectively. These two series represent new drug discovery opportunities for chemical optimization. The results described herein demonstrate the power of combining ALIS with cell functional assays in a high-throughput, target-based approach to determine the most tractable drug discovery opportunities within a pathway. © 2016 Society for Laboratory Automation and Screening.

  16. Analytical Method for the Identification and Assay of Kojic Acid, Methylparaben, and Propylparaben in Cosmetic Products Using UPLC: Application of ISO 12787:2011 Standard.

    Science.gov (United States)

    Qadir, Muhammad Abdul; Ahmed, Mahmood; Shafiq, Muhammad Imtiaz; Ali, Amir; Sadiq, Asma

    2016-09-01

    A straightforward, fast UPLC method is developed for the identification and quantification of kojic acid (KA), methylparaben (MP), and propylparaben (PP) in 15 cosmetic products (skin whitening creams and lotions). Chromatographic separations for KA, MP, and PP were obtained in 3.5 min on an Acquity BEH-C18 column (100 × 2.1 mm, 1.7 μm particle size) as the stationary phase at 260 nm (diode-array detector), with the mobile phase comprising a mixture of 0.01 M dibasic potassium phosphate and methanol-acetonitrile (50 + 50). Validation studies were performed according to in-house established criteria. There was a linear function of concentrations over the range of 0.4-1.6 μg/mL for KA, MP, and PP. The LOQ for all components was 0.2 μg/mL using the S/N method. Good separation of analytes was observed, with acceptable values of resolution and tailing. The analytical approach defined in the ISO 12787:2011 standard ("Cosmetics-Analytical methods-Validation criteria for analytical results using chromatographic techniques") was used for the assay of cosmetic samples.

  17. Evaluation of the international reference methods NF EN ISO 11290-1 and 11290-2 and an in-house method for the isolation of Listeria monocytogenes from retail seafood products in france.

    Science.gov (United States)

    Midelet-Bourdin, Graziella; Leleu, Guylaine; Malle, Pierre

    2007-04-01

    Retail seafood products were analyzed on their use-by date using the international reference methods NF EN ISO 11290-1 and 11290-2 (collectively method R) or an in-house method (method B) for the isolation of Listeria monocytogenes. The sensitivity of the methods was about 78%. Method R detected more positive samples of smoked salmon and herb-flavored slices of smoked salmon than did method B, whereas the reverse was true for samples of carpaccio-like salmon, herb-flavored slices of raw salmon, and smoked trout. Most products produced a positive result after the first of two enrichments, and little difference was observed after changing the isolation medium (Listeria selective agar, L. monocytogenes blood agar, agar for Listeria according to Ottaviani and Agosti, Oxford agar, and Palcam agar). L. monocytogenes was isolated from 151 (27.8%) of the 543 samples, with concentrations mostly below 100 CFU/g. The pathogen prevalence and concentration in these seafood products varied greatly depending on the producer and the nature of the product. In certain cases, these differences could be explained by problems in cleaning and disinfection operations in the food-processing environment. The identities of L. monocytogenes isolates were confirmed by PCR, and isolates were characterized by random amplification of polymorphic DNA and pulsed-field gel electrophoresis (PFGE). PFGE patterns obtained with the enzymes Apal and AscI produced 26 different pulsotypes. In general, different pulsotypes were present in the different categories of seafood products and were not specific to one producer. The genetic diversity observed in the products was not related to the prevalence found at the manufacturing site. It is therefore important for producers to determine the source(s) of contamination of their product so the risks linked to the presence of L. monocytogenes can be reduced.

  18. ISO 14001

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2004-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001, the international standard for environmental management systems (EMS). The standard is a process standard that leaves room for interpretation at company level as well as among lead auditors from certifying bodies....... The paper questions whether the interpretation of the standard is uniform both at national and international levels. The paper also suggests changes of ISO 14001 in order to formulate more clear demands of environmental improvements, to include demand for publication of an environmental report...... and to include product focus more clearly. Secondly the paper briefly presents a cases study of four companies in the automotive industry in South Africa, related to the discussions of ISO 14001 and compliance with environmental regulation....

  19. [Method validation according to ISO 15189 and SH GTA 04: application for the detection of KRAS mutations using PCR TaqMan assay].

    Science.gov (United States)

    Harlé, Alexandre; Dubois, Cindy; Rouyer, Marie; Merlin, Jean-Louis

    2013-01-01

    Since January 16(th) 2010, the French legislation requires that the medical laboratories must be accredited according to ISO 15189 standards. Thus, all medical laboratories in France must be accredited for at least part of their biological tests before the end of October 2013. Molecular biology tests are also concerned by the accreditation. Validation of molecular biology methods is made difficult, for reasons related to the methods, but also by the type of analytes that are basically rare. This article describes the validation of the qualitative detection of KRAS mutations in metastatic colorectal cancer using TaqMan PCR according to ISO 15189 and to the technical guide for accreditation in Human Health, SH-GTA-04, edited by the COFRAC.

  20. [Method validation according to ISO 15189 and SH GTA 04: application for the extraction of DNA and its quantitative evaluation by a spectrophotometric assay].

    Science.gov (United States)

    Harlé, Alexandre; Lion, Maëva; Husson, Marie; Dubois, Cindy; Merlin, Jean-Louis

    2013-01-01

    According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecular biology and specifically in somatic or constitutional genetic. The extracted DNA must meet a number of criteria such quality and also be in sufficient concentration to allow molecular biology assays such as the detection of somatic mutations. This paper describes the validation of the extraction and purification of DNA using chromatographic column extraction and quantitative determination by spectrophotometric assay, according to ISO 15189 and the accreditation technical guide in Human Health SH-GTA-04.

  1. NF EN ISO 11137-1, July 2006. Sterilization of health care products - Irradiation - Part 1: Requirements for development, validation and routine control of a sterilization process for medical devices; NF EN ISO 11137-1, Juillet 2006. Sterilisation des produits de sante - Irradiation - Partie 1: exigences relatives a la mise au point, a la validation et au controle de routine d'un procede de sterilisation pour les dispositifs medicaux

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-07-01

    This part of the ISO 11137 standard specifies requirements for the development, validation and routine control of a radiation sterilization process for medical devices. Although the scope of this part of the ISO 11137 standard is limited to medical devices, it specifies requirements and provides guidance that may be applicable to other products and equipment. This part of the international standard covers radiation processes employing irradiators using the radionuclide {sup 60}Co or {sup 137}Cs, a beam from an electron generator or a beam from an X-ray generator. This part of the international standard does not: - specify requirements for development, validation and routine control of a process for inactivating the causative agents of spongiform encephalopathies such as scrapie, bovine spongiform encephalopathy and Creutzfeldt-Jakob disease; - detail specified requirements for designating a medical device as sterile; - specify a quality management system for the control of all stages of production of medical devices; - specify requirements for occupational safety associated with the design and operation of irradiation facilities; - specify requirements for the sterilization of used or reprocessed devices.

  2. ISO27001 / ISO27002 a pocket guide

    CERN Document Server

    Calder, Alan

    2013-01-01

    Information is one of your organisation's most important resources. Keeping it secure is therefore vital to your business. This handy pocket guide is an essential overview of two key information security standards that cover the formal requirements (ISO27001:2013) for creating an Information Security Management System (ISMS), and the best-practice recommendations (ISO27002:2013) for those responsible for initiating, implementing or maintaining it.

  3. Analytical method for the identification and assay of 12 phthalates in cosmetic products: application of the ISO 12787 international standard "Cosmetics-Analytical methods-Validation criteria for analytical results using chromatographic techniques".

    Science.gov (United States)

    Gimeno, Pascal; Maggio, Annie-Françoise; Bousquet, Claudine; Quoirez, Audrey; Civade, Corinne; Bonnet, Pierre-Antoine

    2012-08-31

    Esters of phthalic acid, more commonly named phthalates, may be present in cosmetic products as ingredients or contaminants. Their presence as contaminant can be due to the manufacturing process, to raw materials used or to the migration of phthalates from packaging when plastic (polyvinyl chloride--PVC) is used. 8 phthalates (DBP, DEHP, BBP, DMEP, DnPP, DiPP, DPP, and DiBP), classified H360 or H361, are forbidden in cosmetics according to the European regulation on cosmetics 1223/2009. A GC/MS method was developed for the assay of 12 phthalates in cosmetics, including the 8 phthalates regulated. Analyses are carried out on a GC/MS system with electron impact ionization mode (EI). The separation of phthalates is obtained on a cross-linked 5%-phenyl/95%-dimethylpolysiloxane capillary column 30 m × 0.25 mm (i.d.) × 0.25 mm film thickness using a temperature gradient. Phthalate quantification is performed by external calibration using an internal standard. Validation elements obtained on standard solutions, highlight a satisfactory system conformity (resolution>1.5), a common quantification limit at 0.25 ng injected, an acceptable linearity between 0.5 μg mL⁻¹ and 5.0 μg mL⁻¹ as well as a precision and an accuracy in agreement with in-house specifications. Cosmetic samples ready for analytical injection are analyzed after a dilution in ethanol whereas more complex cosmetic matrices, like milks and creams, are assayed after a liquid/liquid extraction using ter-butyl methyl ether (TBME). Depending on the type of cosmetics analyzed, the common limits of quantification for the 12 phthalates were set at 0.5 or 2.5 μg g⁻¹. All samples were assayed using the analytical approach described in the ISO 12787 international standard "Cosmetics-Analytical methods-Validation criteria for analytical results using chromatographic techniques". This analytical protocol is particularly adapted when it is not possible to make reconstituted sample matrices. Copyright © 2012

  4. Identification and comparison of anti-inflammatory ingredients from different organs of Lotus nelumbo by UPLC/Q-TOF and PCA coupled with a NF-κB reporter gene assay.

    Directory of Open Access Journals (Sweden)

    Mengge Zhou

    Full Text Available Lotus nelumbo (LN (Nelumbo nucifera Gaertn. is an aquatic crop that is widely distributed throughout Asia and India, and various parts of this plant are edible and medicinal. It is noteworthy that different organs of this plant are used in traditional herbal medicine or folk recipes to cure different diseases and to relieve their corresponding symptoms. The compounds that are contained in each organ, which are named based on their chemical compositions, have led to their respective usages. In this work, a strategy was used to identify the difference ingredients and screen for Nuclear-factor-kappaB (NF-κB inhibitors with anti-inflammatory ability in LN. Seventeen main difference ingredients were compared and identified from 64 samples of 4 different organs by ultra-performance liquid chromatography that was coupled with quadrupole/time of flight mass spectrometry (UPLC/Q-TOF-MS with principal component analysis (PCA. A luciferase reporter assay system combined with the UPLC/Q-TOF-MS information was applied to screen biologically active substances. Ten NF-κB inhibitors from Lotus plumule (LP extracts, most of which were isoquinoline alkaloids or flavone C-glycosides, were screened. Heat map results showed that eight of these compounds were abundant in the LP. In conclusion, the LP extracts were considered to have the best anti-inflammatory ability of the four LN organs, and the chemical material basis (CMB of this biological activity was successfully validated by multivariate statistical analysis and biological research methods.

  5. NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression

    Directory of Open Access Journals (Sweden)

    Yan Li

    2016-02-01

    Full Text Available A previous proteomic screen in our laboratory identified nuclear factor 45 (NF45 and nuclear factor 90 (NF90 as potential cellular factors involved in human immunodeficiency virus type 1 (HIV-1 replication. Both are RNA binding proteins that regulate gene expression; and NF90 has been shown to regulate the expression of cyclin T1 which is required for Tat-dependent trans-activation of viral gene expression. In this study the roles of NF45 and NF90 in HIV replication were investigated through overexpression studies. Ectopic expression of either factor potentiated HIV infection, gene expression, and virus production. Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. Both proteins were found to interact with the HIV RNA. RNA decay assays demonstrated that NF90, but not NF45, increased the half-life of the HIV RNA. Overall, these studies indicate that both NF45 and NF90 potentiate HIV infection through their RNA binding domains.

  6. Control: the effects of ISO

    NARCIS (Netherlands)

    Laura Ilzarbe; PhD Everard van Kemenade; Maria-Jezus Alvarez

    2007-01-01

    De effecten van ISO-certificering zoals gerapporteerd in wetenschappelijk onderzoek. Met speciale aandacht voor de resultaten in het onderwijs. ISO 9000:2000 doet het nadrukkelijk beter dan haar vorige versies.

  7. Accreditation - ISO/IEC 17025

    Science.gov (United States)

    Kaus, Rüdiger

    This chapter gives the background on the accreditation of testing and calibration laboratories according to ISO/IEC 17025 and sets out the requirements of this international standard. ISO 15189 describes similar requirements especially tailored for medical laboratories. Because of these similarities ISO 15189 is not separately mentioned throughout this lecture.

  8. ISO CD 14492

    DEFF Research Database (Denmark)

    Martins, Bo; Kossentini, F.; Arps, R.

    1998-01-01

    This Recommendation | International Standard, informally called JBIG2, defines a coding method for bilevel images, that is, images consisting of a single rectangular bit plane, with each pixel taking on one of just two possible colors. Itis being drafted by the Joint Bi-level Image Experts Group ...... (JBIG), a "Collaborative team" that reports both to ISO/IEC JTC1/SC29/WG1 and to ITU-T/SG8....

  9. ISO 21500 AND PMBOK COMPARISON

    OpenAIRE

    Dotsenko A.V.

    2013-01-01

    On 31st December 2012, the Project Management Institute (PMI) published the 5th edition of the Project Management Body of Knowledge (PMBoK) Guide. A few months earlier, the International Organization for Standardization (ISO) published a new standard, ISO 21500:2012, Guidance on Project Management. The two releases of the PMBoK Guide and ISO 21500 standard are very close. Detailed review of the differences and similarities between them are compiled.

  10. ISO 9000 Quality Management System

    Science.gov (United States)

    Hadjicostas, Evsevios

    The ISO 9000 series describes a quality management system applicable to any organization. In this chapter we present the requirements of the standard in a way that is as close as possible to the needs of analytical laboratories. The sequence of the requirements follows that in the ISO 9001:2008 standard. In addition, the guidelines for performance improvement set out in the ISO 9004 are reviewed. Both standards should be used as a reference as well as the basis for further elaboration.

  11. ISO 50001 Energy Management Standard

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-08-12

    This powerful standard from the International Organization for Standardization (ISO) provides an internationally recognized framework for organizations to voluntarily implement an energy management system.

  12. ISO 19152 : 2012, Land Administration Domain Model published by ISO

    NARCIS (Netherlands)

    Van Oosterom, P.; Lemmen, C.; Uitermark, H.

    2013-01-01

    This paper describes the last developments of the Land Administration Domain Model (LADM). The Final Draft International Standard, ISO FDIS 19152, unanimously passed on 1 November 2012 the final vote towards becoming an International Standard (IS). After technical editing by ISO secretariat in

  13. Quality Assurance through ISO 9000.

    Science.gov (United States)

    Zuckerman, Amy

    2000-01-01

    Created in 1987 by the International Organization for Standardization, in Geneva, Switzerland, ISO 9000 is attempting to develop a world standard to help companies and other institutions measure and monitor their quality-control efforts. This article describes four school districts' successful efforts to secure ISO 9000 certification. (MLH)

  14. ISO 9000: The Librarian's Role.

    Science.gov (United States)

    Dobson, Chris; Ernst, Carolyn

    1999-01-01

    Describes the special library's role in implementing ISO 9000 (i.e., a series of international quality-assurance standards developed by the International Organization of Standards). Topics discussed include document and data control, keeping the standards current, documentation of procedures, the ISO 9000 audit, and benefits for the library. (MES)

  15. ISO 9001 quality management systems

    CERN Document Server

    Natarajan, Dhanasekharan

    2017-01-01

    This book explains the requirements of ISO 9001 for establishing quality management system (QMS) for an organization. The requirements are illustrated with examples from industries for understanding the requirements and preparing the documents of QMS with high clarity. Methods of integrating ISO 9001 requirements with enterprise resource planning (ERP) software are presented. The software integrated approach enables process owners to focus on their core tasks of achieving the planned outputs of processes and the software generates quality records automatically.

  16. Shikonin ameliorates isoproterenol (ISO)-induced myocardial damage through suppressing fibrosis, inflammation, apoptosis and ER stress.

    Science.gov (United States)

    Yang, Jun; Wang, Zhao; Chen, Dong-Lin

    2017-09-01

    Shikonin, isolated from the roots of herbal plant Lithospermum erythrorhizon, is a naphthoquinone. It has been reported to exert beneficial anti-inflammatory effects and anti-oxidant properties in various diseases. Isoproterenol (ISO) has been widely used to establish cardiac injury in vivo and in vitro. However, shikonin function in ISO-induced cardiac injury remains uncertain. In our study, we attempted to investigate the efficiency and possible molecular mechanism of shikonin in cardiac injury treatment induced by ISO. In vivo, C57BL6 mice were subcutaneously injected with 5mg/kg ISO to induce heart failure. And mice were given a gavage of shikonin (2 or 4mg/kg/d, for four weeks). Cardiac function, fibrosis indices, inflammation response, apoptosis and endoplasmic reticulum (ER) stress were calculated. Pathological alterations, fibrosis-, inflammation-, apoptosis- and ER stress-related molecules were examined. In ISO-induced cardiac injury, shikonin significantly ameliorated heart function, decreased myocardial fibrosis, suppressed inflammation, attenuated apoptosis and ER stress through impeding collagen accumulation, Toll like receptor 4/nuclear transcription factor κB (TLR4/NF-κB), Caspase-3 and glucose-regulated protein 78 (GRP78) signaling pathways activity, relieving heart failure in vivo. Also, in vitro, shikonin attenuated ISO-induced cardiac muscle cells by reducing fibrosis, inflammation, apoptosis and ER stress. Our findings indicated that shikonin treatment attenuated ISO-induced heart injury, providing an effective therapeutic strategy for heart failure treatment for future. Copyright © 2017. Published by Elsevier Masson SAS.

  17. Integrated management system: The integration of ISO 9001, ISO 14001, OHSAS 18001 and ISO 31000

    Science.gov (United States)

    Muzaimi, Hafizzudin; Chew, Boon Cheong; Hamid, Syaiful Rizal

    2017-03-01

    The implementation of integrated management system (IMS) for better quality management has become a preference for many organizations. This can be seen as many organizations used the combination of quality ISO 9001, an environment ISO 14001 and occupational health and safety management system OHSAS 18001 as a core for the IMS that largely implemented. Besides, the linked between quality management with risk management system need to be identified as the management system that enhance the effectiveness of IMS. Therefore, the risk management system ISO 31000 also presented as a part of integration. In nowadays competitive environment, the increasing pressure and needs from customer or stakeholders make it compulsory for the organization to propose the new system and standards. This paper presents and discusses about the benefit of integration, the management system components that can be converged and the implementation approach. A series of interview was conducted through in-depth interviews with 8 experts in this field, while data collected were analyzed qualitatively. The results consist of 16 factors of IMS implementation that have been identified and the use of PDCA approach for an effective implementation of IMS. As a conclusion, the paper proposes the integration of four management systems (ISO 9001, ISO 14001, OHSAS 18001 and ISO 31000) and on how the IMS can be used to structure the process of management for quality management towards sustainability practices in the organization.

  18. Tinjauan Kritis terhadap Implementasi Iso 9000

    OpenAIRE

    Nursya’bani Purnama

    2009-01-01

    ISO 9000 telah diterima secara luas sebagai acuan sistem manajemen kualitas dan penjaminan kualitas. Perusahaan-perusahaan yang memperoleh sertifikasi ISO dari tahun ke ke tahun mengalami pertumbu¬han yang pesat. Dengan implementasi dan memperoleh sertifikasi ISO 9000, banyak perusahaan di dunia mem¬peroleh peningkatan kinerja. Meskipun demikian, implementasi ISO 9000 juga dihadapkan pada sejumlah ham¬batan. Implementasi ISO 9000 seringkali dikaitkan dengan Total Quality Management (TQM). Ban...

  19. Tinjauan Kritis Terhadap Implementasi Iso 9000

    OpenAIRE

    Purnama, Nursya’bani

    2005-01-01

    ISO 9000 telah diterima secara luas sebagai acuan sistem manajemen kualitas dan penjaminan kualitas. Perusahaan-Perusahaan yang memperoleh sertifikasi ISO dari tahun ke ke tahun mengalami pertumbu¬han yang pesat. Dengan implementasi dan memperoleh sertifikasi ISO 9000, banyak Perusahaan di dunia mem¬peroleh peningkatan kinerja. Meskipun demikian, implementasi ISO 9000 juga dihadapkan pada sejumlah ham¬batan. Implementasi ISO 9000 seringkali dikaitkan dengan Total Quality Management (TQM). Ban...

  20. [ISO 15189 medical laboratory accreditation].

    Science.gov (United States)

    Aoyagi, Tsutomu

    2004-10-01

    This International Standard, based upon ISO/IEC 17025 and ISO 9001, provides requirements for competence and quality that are particular to medical laboratories. While this International Standard is intended for use throughout the currently recognized disciplines of medical laboratory services, those working in other services and disciplines will also find it useful and appropriate. In addition, bodies engaged in the recognition of the competence of medical laboratories will be able to use this International Standard as the basis for their activities. The Japan Accreditation Board for Conformity Assessment (AB) and the Japanese Committee for Clinical Laboratory Standards (CCLS) are jointly developing the program of accreditation of medical laboratories. ISO 15189 requirements consist of two parts, one is management requirements and the other is technical requirements. The former includes the requirements of all parts of ISO 9001, moreover it includes the requirement of conformity assessment body, for example, impartiality and independence from any other party. The latter includes the requirements of laboratory competence (e.g. personnel, facility, instrument, and examination methods), moreover it requires that laboratories shall participate proficiency testing(s) and laboratories' examination results shall have traceability of measurements and implement uncertainty of measurement. Implementation of ISO 15189 will result in a significant improvement in medical laboratories management system and their technical competence. The accreditation of medical laboratory will improve medical laboratory service and be useful for patients.

  1. α-Iso-cubebene exerts neuroprotective effects in amyloid beta stimulated microglia activation.

    Science.gov (United States)

    Park, Sun Young; Park, Se Jin; Park, Nan Jeong; Joo, Woo Hong; Lee, Sang-Joon; Choi, Young-Whan

    2013-10-25

    Schisandra chinensis is commonly used for food and as a traditional remedy for the treatment of neuronal disorders. However, it is unclear which component of S. chinensis is responsible for its neuropharmacological effects. To answer this question, we isolated α-iso-cubebene, a dibenzocyclooctadiene lignin, from S. chinensis and determined if it has any anti-neuroinflammatory and neuroprotective properties against amyloid β-induced neuroinflammation in microglia. Microglia that are stimulated by amyloid β increased their production of pro-inflammatory cytokines and chemokines, prostaglandin E2 (PGE2), nitric oxide (NO) and reactive oxygen species (ROS) and the enzymatic activity of matrix metalloproteinase 9 (MMP-9). We found this was all inhibited by α-iso-cubebene. Consistent with these results, α-iso-cubebene inhibited the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) and MMP-9 in amyloid β-stimulated microglia. Subsequent mechanistic studies revealed that α-iso-cubebene inhibited the phosphorylation and degradation of IκB-α, the phosphorylation and transactivity of NF-κB, and the phosphorylation of MAPK in amyloid β-stimulated microglia. These results suggest that α-iso-cubebene impairs the amyloid β-induced neuroinflammatory response of microglia by inhibiting the NF-κB and MAPK signaling pathways. Importantly, α-iso-cubebene can provide critical neuroprotection for primary cortical neurons against amyloid β-stimulated microglia-mediated neurotoxicity. To the best of our knowledge, this is the first report showing that α-iso-cubebene can provide neuroprotection against, and influence neuroinflammation triggered by, amyloid β activation of microglia. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  2. ISO 14001: time for improvements?

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2007-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001:2004, the international standard for Environmental Management Systems (EMS) with the purpose of improving the next edition in order to recognise and reflect new recognitions in approaches to pollution prevention. A case...... study is presented and shows lack of life cycle thinking in product development. This paper suggests changes of ISO 14001:2004 in order to include a more product-oriented approach and a stronger focus on stakeholders. It also suggests to formulate clearer demands for targets and environmental...

  3. Environmental Management System ISO 14001

    CERN Document Server

    Haider, Syed

    2010-01-01

    This is a must-have tool for any company in the process of adopting and incorporating the ISO 14001:2004 requirements, this book and accompanying CD-ROM provides the latest updates and amendments and translates the ISO language into actionable strategy. Offering administrative solutions to managers of all sorts, it includes practical examples of policies with objectives, targets, and action plans applicable to any process related industry or an ordinary organization. With template formats and hands-on flow charts to describe step-by-step system development, documentation, and implementation ph

  4. Information security risk management for ISO27001/ISO27002

    CERN Document Server

    Calder, A; Watkins, S

    2010-01-01

    Drawing on international best practice, including ISO/IEC 27005, NIST SP800-30 and BS7799-3, the book explains in practical detail how to carry out an information security risk assessment. It covers key topics, such as risk scales, threats and vulnerabilities, selection of controls, and roles and responsibilities, and includes advice on choosing risk assessment software.

  5. Food service compliance with ISO 14001 and ISO 22000

    Directory of Open Access Journals (Sweden)

    Laura Lyra Santos

    2012-06-01

    Full Text Available OBJECTIVE: The objective of this study was to assess food service environmental and food safety management systems according to two checklists based on ABNT ISO 22000 and 14001. METHODS: This exploratory and descriptive study investigated a-la-carte food services of the Federal District, Brazil. Two checklists were developed to investigate ISO 14001 and 22000 compliance. A total of 37 food services were selected from the list of the Brazilian Association of Bars and Restaurants by simple random sampling. Checklist results were analyzed according to ANVISA resolution nº 275/2002. RESULTS: Only five food services employed dietitians to supervise meal production. These establishments achieved the highest ISO compliance. However, no establishment had more than 50% ISO 14001 or 22000 compliance. Restaurants showed little concern for the environment and disobeyed waste disposal laws by not separating recyclables from non-recyclables. CONCLUSION: The study food services do not have safe meal production systems, evidenced by non-conformity with the reference standards. Additionally, they do not attempt to reduce the environmental impact of their wastes. Food services supervised by dietitians are better prepared to produce safe foods.

  6. Ny versjon av standarden ISO 15489

    OpenAIRE

    Herbjørn Andresen

    2016-01-01

    Standarden NS-ISO 15489-1:2016 er gjeldende norsk standard fra 1. juli 2016. Dette er andre versjon av ISO 15489, den første versjonen fra 2001 er ikke lenger gyldig. 2001-utgaven av ISO 15489 besto av to deler. Del 2, SN-ISO/TR 15489-2:2001 er trukket, slik at den nye ISO 15489 kun består av del 1. ISO 15489 var den første internasjonale Records Management-standarden, og har en funksjon som overordnet referansestandard for en voksende portefølje av senere standarder på tilgrensende områder. ...

  7. Angiogenesis and Therapeutic Approaches to NF1 Tumors

    Science.gov (United States)

    2007-04-01

    Medium Wild-type ECGS Nf1+/- ECGS Wild-type bFGF Nf1+/- bFGF Wild-type VEGF NF1+/- VEGF Mitogen treatment Fig. 1. BrdU incorporation assay of brain...anesthetized, and the sciatic nerves of both legs were exposed at midthigh. The cell suspension (5 ll, 5 3 105 cells) was incrementally injected within the...3. Fascicle 24. Washington, DC: Armed Forces Insti- tute of Pathology. p 385–405. Scott JE, Mowry RW. 1970. Alcian blue—a consumer’s guide. J Histo

  8. Our Application of ISO 9000.

    Science.gov (United States)

    Hammond, Jane

    2000-01-01

    Since a large, underfunded urban Colorado district initiated ISO 9000 reforms, administrators and staff have reviewed 14 central-office departments' processes to improve efficiency and enhance student outcomes. Jefferson County has saved $900,000 annually on purchasing processes, developed a quality curriculum-development process, and improved…

  9. Application Security in the ISO27001 Environment

    CERN Document Server

    Vinod, Vasudevan; Firosh, Ummer

    2008-01-01

    Application Security in the ISO27001 Environment demonstrates how to secure software applications within a best practice ISO/IEC 27001 environment and supports implementation of the PCI DSS Payment Application Security Standard.

  10. The European Large Area ISO Survey

    DEFF Research Database (Denmark)

    Oliver, S.; Rowan-Robinson, M.; Alexander, D.M.

    2000-01-01

    We describe the European Large Area ISO Survey (ELAIS). ELAIS was the largest single Open Time project conducted by ISO, mapping an area of 12 deg(2) at 15 mu m with ISOCAM and at 90 mu m with ISOPHOT. Secondary surveys in other ISO bands were undertaken by the ELAIS team within the fields...

  11. Lipoamide Inhibits NF1 Deficiency-induced Epithelial-Mesenchymal Transition in Murine Schwann Cells.

    Science.gov (United States)

    Zhang, Yuchen; Zhou, Rongsheng; Qu, Yiping; Shu, Maoguo; Guo, Shuzhong; Bai, Zhuanli

    2017-11-29

    Neurofibromatosis type I (NF1) is one of the most common neurocutaneous syndromes characterized by development of adult neurofibromas which is mainly made up of Schwann cells. The disease is generally accepted to be caused by inactivation mutation of Nf1 gene. And Nf1 deficiency had been reported to lead to ROS overproduction and epithelial-mesenchymal transition (EMT) phenotype. This study was designed to investigate whether excessive ROS conferred to Nf1 deficiency-induced EMT in Schwann cells. Colony formation, wound healing assay and transwell assay was used to evaluate the effects of stable Nf1 knockdown in SW10 Schwann cells. Western blot and ROS assay was conducted to explore the molecular mechanisms of Nf1 inactivation in tumorigenesis. Animal experiments were performed to assess the inhibitory effects of lipoamide, which is the neutral amide of α-lipoic acid and functions as a potent antioxidant to scavenge ROS, on Nf1-deficiency tumor growth in vivo. Nf1 knockdown enhanced the cellular capacities of proliferation, migration and invasion, promoted ROS generation, decreased the expression of epithelial surface marker E-cadherin, and up-regulated several EMT-associated molecules in Schwann cells. Moreover, lipoamide dose-dependently inhibited not only Nf1 deficiency-induced EMT but also spontaneous EMT. Furthermore, lipoamide markedly suppresses tumor growth in a mouse model of NF1-associated neurofibroma. Our results clearly reveal that ROS overproduction is responsible for Nf1 deficiency-induced EMT and plays a crucial role in NF1 tumor growth. The findings presented herein shed light on the potential of antioxidant therapy to prevent the progression of NF1-associated neurofibroma. Copyright © 2017 IMSS. Published by Elsevier Inc. All rights reserved.

  12. A Transgenic Model for Learning Defects: Role of NF1 in Drosophila Visual-Spatial Learning

    Science.gov (United States)

    2008-12-01

    segments of different orientations and elevations, prior to assaying NF1 mutants. We will begin to prepare a manuscript for publication that describes...Role of NF1 in Drosophila Visual- Spatial Learning PRINCIPAL INVESTIGATOR: Frances Hannan, Ph.D. CONTRACTING...Model for Learning Defects: Role of NF1 in Drosophila Visual-Spatial Learnings 5b. GRANT NUMBER W81XWH-07-1-0436 5c. PROGRAM ELEMENT NUMBER 6

  13. Ny versjon av standarden ISO 15489

    Directory of Open Access Journals (Sweden)

    Herbjørn Andresen

    2016-09-01

    Full Text Available Standarden NS-ISO 15489-1:2016 er gjeldende norsk standard fra 1. juli 2016. Dette er andre versjon av ISO 15489, den første versjonen fra 2001 er ikke lenger gyldig. 2001-utgaven av ISO 15489 besto av to deler. Del 2, SN-ISO/TR 15489-2:2001 er trukket, slik at den nye ISO 15489 kun består av del 1. ISO 15489 var den første internasjonale Records Management-standarden, og har en funksjon som overordnet referansestandard for en voksende portefølje av senere standarder på tilgrensende områder. Denne artikkelen gjennomgår endringene i hovedtrekk, både i standarden ISO 15489 som sådan og i noen av de standardene som bygger videre på den.

  14. International Organization for Standardization (ISO) 15189

    OpenAIRE

    Schneider, Frank; Maurer, Caroline; Friedberg, Richard C.

    2017-01-01

    The College of American Pathologists (CAP) offers a suite of laboratory accreditation programs, including one specific to accreditation to the international organization for standardization (ISO) 15189 standard for quality management specific to medical laboratories. CAP leaders offer an overview of ISO 15189 including its components, internal audits, occurrence management, document control, and risk management. The authors provide a comparison of its own ISO 15189 program, CAP 15189, to the ...

  15. Sistemas de referencia e identificadores geográficos (ISO 19111, ISO 19112, ISO 6709)

    OpenAIRE

    Sevilla, Miguel J.; Iguácel Abeigón, Cristina; Abad Power, Paloma

    2008-01-01

    Para la familia de normas ISO 19100 describir de manera normalizada, consistente, no ambigua y eficaz posiciones geográficas, es decir posiciones relativas a la superficie terrestre, es fundamental. El posicionamiento es el rasgo diferenciador de la IG, y su normalización un elemento clave para la interoperabilidad de los sistemas. Por ello, se trata de un aspecto esencial y, en ese sentido pueden considerarse básicas las normas que atañen a este propósito de una manera más dir...

  16. International Organization for Standardization (ISO) 15189.

    Science.gov (United States)

    Schneider, Frank; Maurer, Caroline; Friedberg, Richard C

    2017-09-01

    The College of American Pathologists (CAP) offers a suite of laboratory accreditation programs, including one specific to accreditation to the international organization for standardization (ISO) 15189 standard for quality management specific to medical laboratories. CAP leaders offer an overview of ISO 15189 including its components, internal audits, occurrence management, document control, and risk management. The authors provide a comparison of its own ISO 15189 program, CAP 15189, to the CAP Laboratory Accreditation Program. The authors conclude with why laboratories should use ISO 15189. © The Korean Society for Laboratory Medicine.

  17. Plasma 8-iso-Prostaglandin F2α concentrations and outcomes after acute intracerebral hemorrhage.

    Science.gov (United States)

    Du, Quan; Yu, Wen-Hua; Dong, Xiao-Qiao; Yang, Ding-Bo; Shen, Yong-Feng; Wang, Hao; Jiang, Li; Du, Yuan-Feng; Zhang, Zu-Yong; Zhu, Qiang; Che, Zhi-Hao; Liu, Qun-Jie

    2014-11-01

    Higher plasma 8-iso-Prostaglandin F2α concentrations have been associated with poor outcome of severe traumatic brain injury. We further investigated the relationships between plasma 8-iso-Prostaglandin F2α concentrations and clinical outcomes in patients with acute intracerebral hemorrhage. Plasma 8-iso-Prostaglandin F2α concentrations of 128 consecutive patients and 128 sex- and gender-matched healthy subjects were measured by enzyme-linked immunosorbent assay. We assessed their relationships with disease severity and clinical outcomes including 1-week mortality, 6-month mortality and unfavorable outcome (modified Rankin Scale score>2). Plasma 8-iso-Prostaglandin F2α concentrations were substantially higher in patients than in healthy controls. Plasma 8-iso-Prostaglandin F2α concentrations were positively associated with National Institutes of Health Stroke Scale (NIHSS) scores and hematoma volume using a multivariate linear regression. It emerged as an independent predictor for clinical outcomes of patients using a forward stepwise logistic regression. ROC curves identified the predictive values of plasma 8-iso-Prostaglandin F2α concentrations, and found its predictive value was similar to NIHSS scores and hematoma volumes. However, it just numerically added the predictive values of NIHSS score and hematoma volume. Increased plasma 8-iso-Prostaglandin F2α concentrations are associated with disease severity and clinical outcome after acute intracerebral hemorrhage. Copyright © 2014 Elsevier B.V. All rights reserved.

  18. IsoBank workshop held at IsoEcol10 - University of Tokyo 2016

    OpenAIRE

    Hayden, Brian

    2016-01-01

    IsoBank is a repository for stable isotope values and relevant metadata. These slides were presented at a workshop held during IsoEcol10 to refine and design the IsoBank for the needs of a diverse group of researchers.

  19. Interactions of the CCAAT-binding trimer NF-Y with nucleosomes.

    Science.gov (United States)

    Motta, M C; Caretti, G; Badaracco, G F; Mantovani, R

    1999-01-15

    NF-Y is a sequence-specific evolutionary conserved activator binding to CCAAT boxes with high affinity and specificity. It is a trimer formed by NF-YA and two putative histone-like subunits, NF-YB and NF-YC, showing similarity to histones H2B and H2A, respectively. We investigated the relationships between NF-Y and chromatin using an Artemia franciscana chromatin assembly system with plasmids containing the Major HistoCompatibility complex class II Ea promoter. The NF-Y trimer, but not single subunits, protects the Y box in the presence of reconstituted chromatin, and it can bind the target sequence during and after assembly. Using reconstitution assays with purified chicken histones, we show that NF-Y associates with preformed nucleosomes. Translational analysis of various Ea fragments of identical length in which the CCAAT box is at different positions indicated that the lateral fragment was slightly more prone to NF-Y binding. In competition experiments, NF-Y is able to prevent formation of nucleosomes significantly. These data support the idea that NF-Y is a gene-specific activator with a built-in capacity to interface with chromatin structures.

  20. Enhancing Technology Education Students' Understanding of ISO 9000.

    Science.gov (United States)

    Harding, Kerry A.; Tesolowski, Dennis G.; Simmons, J. Bryan

    2000-01-01

    Defines ISO 9000, the quality control standard of the International Organization for Standardization (ISO), and looks at the benefits of ISO certification. Looks at the four levels of ISO 9000 and discusses the construction of training courses to prepare ISO documentation. (JOW)

  1. Maximizing Value for Training with ISO 9000.

    Science.gov (United States)

    Russo, C. W. Russ; Russo, Tracy Callaway

    1996-01-01

    The International Organization for Standardization (ISO) has created quality assurance guidelines that help technology trainers and educators manage and organize training programs. This article briefly outlines program design principles, emphasizing needs analysis and outcome evaluation, performance documentation, and process management. ISO 9000…

  2. Documenting an ISO 9000 Quality System.

    Science.gov (United States)

    Fisher, Barry

    1995-01-01

    Discusses six steps to follow when documenting an ISO 9000 quality system: using ISO 9000 to develop a quality system, identifying a company's business processes, analyzing the business processes; describing the procedures, writing the quality manual, and working to the documented procedures. (SR)

  3. Implementation and Impact of ISO 9001

    NARCIS (Netherlands)

    B. Manders (Basak)

    2015-01-01

    markdownabstract__Abstract __ The ISO 9001 quality management standard has been implemented by more than one million organizations in 187 countries since its introduction in 1987. A newer version of ISO 9001 is planned to be introduced in 2015. Even though it is widely used by organizations to

  4. ISO Science Legacy A Compact Review of ISO Major Achievements

    CERN Document Server

    Cesarsky, Catherine J

    2006-01-01

    Stars are born and die in clouds of gas and dust, opaque to most types of radiation, but transparent in the infrared. Requiring complex detectors, space missions and cooled telescopes, infrared astronomy is the last branch of this discipline to come of age. After a very successful sky survey performed in the eighties by the IRAS satellite, the Infrared Space Observatory, in the nineties, brought spectacular advances in the understanding of the processes giving rise to powerful infrared emission by a great variety of celestial sources. Outstanding results have been obtained on the bright comet Hale-Bopp, and in particular of its water spectrum, as well as on the formation, chemistry and dynamics of planetary objects in the solar system. Ideas on the early stages of stellar formation and on the stellar initial mass function have been clarified. ISO is the first facility in space able to provide a systematic diagnosis of the physical phenomena and the chemistry in the close environment of pre-main sequence stars...

  5. IMPLEMENTATION OF ISO 14001 IN BULGARIA

    Directory of Open Access Journals (Sweden)

    Hristina HARIZANOVA

    2015-04-01

    Full Text Available In the last years, the Bulgarian business organizations and government bodies were facing with the increased needs to implement new standards which could bring a higher benefit for companies and also not to affect the environment. The ISO 14 000 Family is based on standards for environmental management and gives the possibility to be applied in any type of organization, public or private sectors, administrations, etc . The main aim of the publication is to trace the implementation of ISO 14001 in Bulgaria by sectors and type of activities. As well this research offers the main reasons to the Bulgarian firms to implement ISO 14001. For reaching the aim, the study made a review of a Evolution of ISO 14001 and its flexibility, in compliance with changes in the environment and business; b Research of the firms which implemented ISO 14001 in Bulgaria, c Content of the activities in the certification cycle. The used methods are according to the research system of the Bulgarian firms which implemented ISO 14001. Finally, some conclusions were drawn about the development of the ISO 14 001 in Bulgaria.

  6. Peningkatan Mutu Proses Perguruan Tinggi Melalui Sistem Mutu Iso 9000

    Directory of Open Access Journals (Sweden)

    Th. Widia Soerjaningsih

    2004-09-01

    Full Text Available Article was made to answer these several questions;  what is acreditation and the application in the college; what is ISO 9000 quality system; why is ISO 9000 needed by the college; how ISO 9000 can support the achievement of the defined quality level; how ISO  9000 guarantee the achievement of BAN target including quality, efficiency, and relevance; what is the difference between BAN Acreditation and ISO 9000 Certification; ISO 9000 Consultant: why it is necessary, how to choose it, the line of the contract; ISO 9000 Assessor and the achieve certification: SGS and etc.; and ISO 9000 Certification Proccess Cost Aspect.   

  7. ISO 9001 2000 : the quality management process

    CERN Document Server

    Tricker, Ray

    2006-01-01

    With the publication of ISO 9001:2000, there is now a single quality management ?requirements? standard that is applicable to all organisations, products and services. ISO 9001:2000 is the only standard that can be used for the certification of a QMS and its generic requirements can be used by any organisation.ISO 9001:2000 applies to all types or organisations. It is the quality standard which specifies the requirements of quality management systems for use where organisations need to demonstrate their capability to provide products and services which meet both customer needs and relevant reg

  8. Borna disease virus possesses an NF-ĸB inhibitory sequence in the nucleoprotein gene.

    Science.gov (United States)

    Makino, Akiko; Fujino, Kan; Parrish, Nicholas F; Honda, Tomoyuki; Tomonaga, Keizo

    2015-03-03

    Borna disease virus (BDV) has a non-segmented, negative-stranded RNA genome and causes persistent infection in many animal species. Previous study has shown that the activation of the IκB kinase (IKK)/NF-κB pathway is reduced by BDV infection even in cells expressing constitutively active mutant IKK. This result suggests that BDV directly interferes with the IKK/NF-κB pathway. To elucidate the mechanism for the inhibition of NF-κB activation by BDV infection, we evaluated the cross-talk between BDV infection and the NF-κB pathway. Using Multiple EM for Motif Elicitation analysis, we found that the nucleoproteins of BDV (BDV-N) and NF-κB1 share a common ankyrin-like motif. When THP1-CD14 cells were pre-treated with the identified peptide, NF-κB activation by Toll-like receptor ligands was suppressed. The 20S proteasome assay showed that BDV-N and BDV-N-derived peptide inhibited the processing of NF-κB1 p105 into p50. Furthermore, immunoprecipitation assays showed that BDV-N interacted with NF-κB1 but not with NF-κB2, which shares no common motif with BDV-N. These results suggest BDV-N inhibits NF-κB1 processing by the 20S proteasome through its ankyrin-like peptide sequence, resulting in the suppression of IKK/NF-κB pathway activation. This inhibitory effect of BDV on the induction of the host innate immunity might provide benefits against persistent BDV infection.

  9. Segmental NF: A Guide for Patients

    Science.gov (United States)

    ... the body can show signs of segmental NF. Segmental NF1 - Individuals with segmental NF1 most commonly have the skin findings associated with ... cases, can include severe complications. Many individuals with segmental NF1 never develop any complications other than café-au- ...

  10. The practical application of ISO 22301.

    Science.gov (United States)

    Zawada, Brian

    2014-01-01

    As well as providing a brief background of ISO 22301, this paper describes three practical uses of the standard, as well as ways to use the content to solve common challenges that business continuity professionals face every day.

  11. ISO 14001 ENVIRONMENTAL MANAGEMENT SYSTEMS FOR GOVERNMENT

    Science.gov (United States)

    This presentation provides an overview of the document, ISO 14001 - A Tool for Supporting Government Environmental Programs and Policies, recently published by USEPA/s Office of Research and Development. It is an informational document, explaining the backjground and development ...

  12. Benefits of the ISO 9001 and ISO 14001 standards: A literature review

    Directory of Open Access Journals (Sweden)

    Juan José Tarí

    2012-12-01

    Full Text Available Purpose: The purpose of this paper is to determine the similarities and differences between the benefits derived from implementing the ISO 9001 and the ISO 14001 standards.Methodology/Approach: The paper reviews the literature using an electronic search in the ScienceDirect, ABI/Inform, Emerald databases to identify papers focusing on the adoption of the ISO 9001 and 14001 standards and the benefits derived from implementing them.Findings: The paper identifies 82 articles about ISO 9001 and 29 about ISO 14001. Although some differences can be observed between the benefits considered by ISO 9001 and 14001, there is a great degree of coincidence in the benefits studied. The review suggests 13 benefits as the most usually analyzed (including environmental performance for the case of the ISO 14001 standard by scholars. It is suggested that both standards have clear benefits on operational, people and customer results and that the effects on financial performance are inconclusive.Limitations/implications: One limitation of this paper is that the works identified are conditioned by the search strategy used. In addition, other key words could be included in future studies such as operational, market, quality, financial performance, and customer satisfaction in order to expand this search.Originality/Value: The main contribution is that the paper identifies the literature gap and future research proposals with regard to the benefits of the ISO 9001 and ISO 14001 standards.

  13. 75 FR 7581 - RTO/ISO Performance Metrics; Notice Requesting Comments on RTO/ISO Performance Metrics

    Science.gov (United States)

    2010-02-22

    ... Energy Regulatory Commission RTO/ISO Performance Metrics; Notice Requesting Comments on RTO/ ISO..., work with regional transmission organizations (RTO), Independent System Operators (ISO), stakeholders and other experts to develop standardized measures that track the performance of RTO/ISO operations...

  14. Promoting Strong ISO 50001 Outcomes with Supportive National Infrastructure:

    OpenAIRE

    McKane, Aimee, T.; Siciliano, Graziella; de los Reyes, Pamela

    2015-01-01

    The ISO 50001 standard is a key mechanism for reducing greenhouse gas emissions and improving energy efficiency globally. An increasing number of companies are seeking certification, creating the need for personnel that are competent to conduct ISO 50001 certification audits. The growth of ISO 50001 is expected to accelerate as more companies integrate ISO 50001 into their corporate sustainability strategies and supplier requirements. Robust implementation of ISO 50001 represents an impo...

  15. Studi Teoritis Iso 9000 Dan Tqm: Substitusi Atau Komplemeter?

    OpenAIRE

    Respati, Harianto

    2007-01-01

    Banyak pertentangan pendapat tentang dampak ISO 9000 dan TQM dalam kaitannya dengan tujuannya yakni kepuasan pelanggan. Selain itu peran ISO 9000 dan TQM dipandang masih membingungkan. Penjelasan pada jurnal ini, diuraikan secara konseptual baik dari pernyataan para pakar maupun hasil-hasil penelitian terdahulu tentang peran ISO 9000 dan TQM. Hasil menujukkan bahwa secara umum ISO 9000 dan TQM mempunyai tujuan yang sama yakni kepuasan pelanggan. Peran implementasi sertifikasi ISO 9000 dan pra...

  16. ISO 9000 and Creativity: Potential Advantages of Implementing ISO in Community Colleges

    Science.gov (United States)

    Alkeaid, Adel

    2007-01-01

    The purpose of this paper is to examine the relationship between the ISO 9000 standard and creativity. In this paper, the researcher tries to answer the following questions: Which learning theories best foster creative thought? Is creativity more behaviorist or constructivist? Where does ISO 9000 fit in the continuum from behaviorism to…

  17. IS ISO 9000 CERTIFICATION LOSING ITS CREDIBILITY?

    Directory of Open Access Journals (Sweden)

    P.R. Ramesh

    2013-03-01

    Full Text Available ISO certification was a great hope for many stakeholders in India for making the country strong in terms of quality practices. It was a blessing in disguise when in early nineties; buyers were imposing conditions that you should be ISO compliant to do business with them. ISO was a great system to bring in improvements in the way an organization functions. But unfortunately some have adopted a short cut approach in getting the certification done. It is only just before the audit team comes for re-inspection that firms start removing dust from the quality manual files so that they can pretend that that they follow those systems. But a real commitment to adhering to these systems is missing. The paper describes such scenario existing in some quarters in India and warns against such practices for the overall good of the system.

  18. Chemical biology strategy reveals pathway-selective inhibitor of NF-kappaB activation induced by protein kinase C.

    Science.gov (United States)

    Shi, Ranxin; Re, Daniel; Dudl, Eric; Cuddy, Michael; Okolotowicz, Karl J; Dahl, Russell; Su, Ying; Hurder, Andrew; Kitada, Shinichi; Peddibhotla, Satyamaheshwar; Roth, Gregory P; Smith, Layton H; Kipps, Thomas J; Cosford, Nicholas; Cashman, John; Reed, John C

    2010-03-19

    Dysregulation of NF-kappaB activity contributes to many autoimmune and inflammatory diseases. At least nine pathways for NF-kappaB activation have been identified, most of which converge on the IkappaB kinases (IKKs). Although IKKs represent logical targets for potential drug discovery, chemical inhibitors of IKKs suppress all known NF-kappaB activation pathways and thus lack the selectivity required for safe use. A unique NF-kappaB activation pathway is initiated by protein kinase C (PKC) that is stimulated by antigen receptors and many growth factor receptors. Using a cell-based high-throughput screening (HTS) assay and chemical biology strategy, we identified a 2-aminobenzimidazole compound, CID-2858522, which selectively inhibits the NF-kappaB pathway induced by PKC, operating downstream of PKC but upstream of IKKbeta, without inhibiting other NF-kappaB activation pathways. In human B cells stimulated through surface immunoglobulin, CID-2858522 inhibited NF-kappaB DNA-binding activity and expression of endogenous NF-kappaB-dependent target gene, TRAF1. Altogether, as a selective chemical inhibitor of the NF-kappaB pathway induced by PKC, CID-2858522 serves as a powerful research tool and may reveal new paths toward therapeutically useful NF-kappaB inhibitors.

  19. ISO 22000 FOOD SAFETY MANAGEMENT SYSTEM

    OpenAIRE

    Başaran, Burhan

    2015-01-01

    The increase in the level of welfare of the countriesand the awareness of the consumers have forced the firms in the food sector toseek for new pursuits. In this re­gard, ISO 22000 is the most commonlypreferred food safety system. This study aims to contribute the share­holdersand the researchers studying this topic by in­terpreting the lateststudies in this field and determine the critical relationships inorder to picture an effective and productive implementation ofISO22000 which is one of ...

  20. ISO Abbreviations for Names of Polymeric Substances

    Directory of Open Access Journals (Sweden)

    V. Jarm

    2011-04-01

    Full Text Available The use of abbreviations for the names of polymers is practical and economic in written and spoken language. Taking into consideration the several hundreds of polymers appearing in literature annually, some of them having complicated structures, it is almost impossible to derive a systematic and unique abbreviation to polymer structures. Therefore, IUPAC has taken over the well-established ISO list of abbreviated terms (about 120 items mainly selected on the basis of the scale of production. The presented ISO nomenclature is not necessarily in accord with IUPAC recommendations.

  1. Involvement of activation of the Nrf2/ARE pathway in protection against 6-OHDA-induced SH-SY5Y cell death by α-iso-cubebenol.

    Science.gov (United States)

    Park, Sun Young; Kim, Do Yeon; Kang, Jong-Koo; Park, Geuntae; Choi, Young-Whan

    2014-09-01

    Free radical-mediated neurodegeneration is one of the many causes of Parkinson's disease (PD). As part of our ongoing studies on the identification of biologically active Schisandra chinensis components, we have isolated and structurally elucidated α-iso-cubebenol. This study was carried out in an attempt to clarify the neuroprotective effect of α-iso-cubebenol on toxin-insulted dopaminergic neuronal death using 6-hydroxy-dopamine (6-OHDA)-induced dopaminergic SH-SY5Y cells. α-iso-cubebenol significantly attenuated the loss of mitochondrial function (MTT assay) and membrane integrity (lactate dehydrogenase assay) associated with 6-OHDA-induced neurotoxicity. Pretreatment of the cells with α-iso-cubebenol diminished the intracellular accumulation of reactive oxygen species (ROS) and calcium in response to 6-OHDA. Moreover, α-iso-cubebenol protected against 6-OHDA-induced neurotoxicity through inhibition of SH-SY5Y cell apoptosis. In addition, JC-1 staining, which is a well-established measure of mitochondrial damage, was decreased after treatment with α-iso-cubebenol. Notably, α-iso-cubebenol inhibited the release of mitochondrial flavoprotein apoptosis inducing factor (AIF) from the mitochondria to the cytosol and nucleus following 6-OHDA treatment. In addition, α-iso-cubebenol reduced the 6-OHDA-induced phosphorylation of ERK and induced the phosphorylation of PKA, PKB, and CREB in a dose-dependent manner. Moreover, α-iso-cubebenol stimulated the activation of Nrf2, a downstream target of CREB. Furthermore, α-iso-cubebenol stimulated the expression of multiple antioxidant response genes (NQO-1 and HO-1). Finally, CREB and Nrf2 siRNA transfection diminished α-iso-cubebenol-mediated neuroprotection. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Postoperative plasma 8-iso-prostaglandin F2α levels are associated with delirium and cognitive dysfunction in elderly patients after hip fracture surgery.

    Science.gov (United States)

    Zheng, Yuan-Bo; Ruan, Guo-Mo; Fu, Jia-Xing; Su, Zhong-Liang; Cheng, Peng; Lu, Jian-Zuo

    2016-04-01

    Oxidative stress may be involved in occurrence of postoperative delirium (POD) and cognitive dysfunction (POCD). 8-iso-Prostaglandin F2α (8-iso-PGF2α), an isoprostane derived from arachidonic acid via lipid peroxidation, is considered a gold standard for measuring oxidative stress. The present study aimed to investigate the ability of postoperative plasma 8-iso-PGF2α levels to predict POD and POCD in elderly patients undergoing hip fracture surgery. Postoperative plasma 8-iso-PGF2α levels of 182 patients were measured by an enzyme-linked immunosorbent assay. We assessed the relationships between plasma 8-iso-PGF2α levels and the risk of POD and POCD using a multivariate analysis. Plasma 8-iso-PGF2α levels and age were identified as the independent predictors for POD and POCD. Based on areas under receiver operating characteristic curve, the predictive values of 8-iso-PGF2α were obviously higher than those of age for POD and POCD. In a combined logistic-regression model, 8-iso-PGF2α significantly enhanced the areas under curve of age for prediction of POD and POCD. Postoperative plasma 8-iso-PGF2α levels may have the potential to predict POD and POCD in elder patients undergoing hip fracture surgery. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. Isorhapontigenin (ISO) inhibited cell transformation by inducing G0/G1 phase arrest via increasing MKP-1 mRNA Stability.

    Science.gov (United States)

    Gao, Guangxun; Chen, Liang; Li, Jingxia; Zhang, Dongyun; Fang, Yong; Huang, Haishan; Chen, Xiequn; Huang, Chuanshu

    2014-05-15

    The cancer chemopreventive property of Chinese herb new isolate isorhapontigenin (ISO) and mechanisms underlying its activity have never been explored. Here we demonstrated that ISO treatment with various concentrations for 3 weeks could dramatically inhibit TPA/EGF-induced cell transformation of Cl41 cells in Soft Agar assay, whereas co-incubation of cells with ISO at the same concentrations could elicit G0/G1 cell-cycle arrest without redundant cytotoxic effects on non-transformed cells. Further studies showed that ISO treatment resulted in cyclin D1 downregulation in dose- and time-dependent manner. Our results indicated that ISO regulated cyclin D1 at transcription level via targeting JNK/C-Jun/AP-1 activation. Moreover, we found that ISO-inhibited JNK/C-Jun/AP-1 activation was mediated by both upregulation of MKP-1 expression through increasing its mRNA stability and deactivating MKK7. Most importantly, MKP-1 knockdown could attenuate ISO-mediated suppression of JNK/C-Jun activation and cyclin D1 expression, as well as G0/G1 cell cycle arrest and cell transformation inhibition, while ectopic expression of FLAG-cyclin D1 T286A mutant also reversed ISO-induced G0/G1 cell-cycle arrest and inhibition of cell transformation. Our results demonstrated that ISO is a promising chemopreventive agent via upregulating mkp-1 mRNA stability, which is distinct from its cancer therapeutic effect with downregulation of XIAP and cyclin D1 expression.

  4. [Update to ISO 15189:2012, and future prospects].

    Science.gov (United States)

    Shimoda, Katsuji; Kubono, Katsuo

    2014-06-01

    ISO (the International Organization for Standardization) is a worldwide federation of national standard bodies (ISO member bodies). The work of preparing international standards is normally carried out through ISO technical committees. ISO 15189 was prepared by Technical Committee ISO/TC 212, clinical laboratory testing, and in vitro diagnostic test systems. This third edition replaces the second edition (ISO 15189:2007), which was technically revised. Medical laboratory services are essential for patient care and, therefore, have to be available to meet the needs of all patients and the clinical personnel responsible for the care of those patients.

  5. NF-κB Inhibitors from Eurycoma longifolia

    Science.gov (United States)

    2014-01-01

    The roots of Eurycoma longifolia have been used in many countries of Southeast Asia to alleviate various diseases including malaria, dysentery, sexual insufficiency, and rheumatism. Although numerous studies have reported the pharmacological properties of E. longifolia, the mode of action of the anti-inflammatory activity has not been elucidated. Bioguided isolation of NF-κB inhibitors using an NF-κB-driven luciferase reporter gene assay led to the identification of a new quassinoid, eurycomalide C (1), together with 27 known compounds including 11 quassinoids (2–12), six alkaloids (13–18), two coumarins (19, 20), a squalene derivative (21), a triterpenoid (22), and six phenolic compounds (23–28) from the extract of E. longifolia. Evaluation of the biological activity revealed that C19-type and C20-type quassinoids, β-carboline, and canthin-6-one alkaloids are potent NF-κB inhibitors, with IC50 values in the low micromolar range, while C18-type quassinoids, phenolic compounds, coumarins, the squalene derivative, and the triterpenoid turned out to be inactive when tested at a concentration of 30 μM. Eurycomalactone (2), 14,15β-dihydroklaieanone (7), and 13,21-dehydroeurycomanone (10) were identified as potent NF-κB inhibitors with IC50 values of less than 1 μM. PMID:24467387

  6. Signal Transducer and Activator of Transcription (STAT)-3 Activates Nuclear Factor (NF)-κB in Chronic Lymphocytic Leukemia Cells

    Science.gov (United States)

    Liu, Zhiming; Hazan-Halevy, Inbal; Harris, David M.; Li, Ping; Ferrajoli, Alessandra; Faderl, Stefan; Keating, Michael J.; Estrov, Zeev

    2014-01-01

    Nuclear factor (NF)-κB plays a major role in the pathogenesis of B-cell neoplasms. A broad array of mostly extracellular stimuli has been reported to activate NF-κB, to various degrees, in chronic lymphocytic leukemia (CLL) cells. Because CLL cells harbor high levels of unphosphorylated (U) signal transducer and activator of transcription (STAT)-3 protein and U-STAT3 was reported to activate NF-κB, we sought to determine whether U-STAT3 activates NF-κB in CLL. Using the electrophoretic mobility shift assay (EMSA) we studied peripheral blood low-density cells from 15 patients with CLL and found that CLL cell nuclear extracts from all the samples bound to an NF-κB DNA probe, suggesting that NF-κB is constitutively activated in CLL. Immunoprecipitation studies showed that STAT3 bound NF-κB p65, and confocal microscopy studies detected U-STAT3/NF-κB complexes in the nuclei of CLL cells, thereby confirming these findings. Furthermore, infection of CLL cells with retroviral STAT3-shRNA attenuated the binding of NF-κB to DNA, as assessed by EMSA, and downregulated mRNA levels of NF-κB-regulated genes, as assessed by quantitative polymerase chain reaction. Taken together, our data suggest that U-STAT3 binds to the NF-κB p50/p65 dimers and that the U-STAT3/NF-κB complexes bind to DNA and activate NF-κB-regulated genes in CLL cells. PMID:21364020

  7. ISO sertifikaat aitab otsustada / Toomas Udu

    Index Scriptorium Estoniae

    Udu, Toomas

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti : Transport i logistika 25. mai lk. 8. Teenuse kvaliteedi näitajad logistikafirmas ISO standardi alusel. Vt. samas: Eestis kasutatakse kolme kvaliteedistandardit; Kuidas mõjutada kvaliteeti läbi juhtimissüsteemi

  8. The Technical Communicator and ISO 9000.

    Science.gov (United States)

    Weiss, Edmond H.

    1993-01-01

    Describes the complexities of ISO 9000, a set of generic quality standards to which any commercial firm wishing to do business with a member firm of the European Economic Community will have to comply. Describes opportunities for professional technical communicators in providing the required product documentation. (SR)

  9. New ISO standards for hearing protectors (A)

    DEFF Research Database (Denmark)

    Poulsen, Torben

    2000-01-01

    Working Group 17 under ISO/TC43/SC1 deals with measurement of hearing protector attenuation. WG17 is presently involved in revision of the present 4869-1 standard (especially sound field requirements and reproducibility estimations) and formulation of a new standard where inexperienced hearing...

  10. ISO 19512 : The land administration domain model

    NARCIS (Netherlands)

    Lemmen, C.H.J.; Van Oosterom, P.J.M.

    2011-01-01

    Focus of this paper is on the Land Administration Domain Model which is under development as an International Standard at ISO. This development is an initiative of the International Federation of Surveyors – FIG. The International Standard is expected to be published in 2012. Why is this development

  11. [ISO 15189 accreditation acquisition and the procedure].

    Science.gov (United States)

    Kayamori, Yuzo; Toyofuku, Mitsuko; Hotta, Taeko; Fujise, Masako; Egashira, Sadaomi; Kang, Dongchon

    2009-02-01

    Recently, the transparency of customer service and organization administration has been required in many fields. Objective evaluation by a third person is necessary. This also applies to medical institutions; whether the responsibility for explanation to patients and the transparency of medical activities are open is currently emphasized. ISO15189, which specifically applies to clinical laboratories, is an international standard issued in 2003. This standard has commonly been employed as an accreditation tool for clinical laboratories in Europe and Australia. In Japan, the Japan Accreditation Board for Conformity Assessment (JAB) introduced this system as an accreditation tool for clinical laboratories in cooperation with the Japanese Committee for Clinical Laboratory Standards (JCCLS) in 2004. In April 2005, accreditation work was started. Among the ISO systems, ISO15189 is perhaps the first standard specialized in the field of medical practice. This reflects the fact that the importance of clinical examination is internationally recognized. In this report, we summarize the process via which our laboratory was awarded this ISO and the situation in which we passed two surveillances.

  12. Enthusiasm for Europe's space telescope ISO

    Science.gov (United States)

    1996-11-01

    "Are the most luminous galaxies powered by extreme rates of star formation, or do they harbour black-hole-powered active nuclei as well?" Scientists lead by Reinhard Genzel at Max Planck Institut fur extra terrestrische Physik in Garching, Germany, have used ISO's Short Wavelength Spectrometer to give an answer. Writing in the London journal Nature, an independent commentator, Gerry Gilmore of the Institute of Astronomy in Cambridge, considers that their papers in Astronomy and Astrophysics give a clear answer to this question. The "remarkable result" according to Gilmore is that none of three ultra-luminous infrared galaxies studied by ISO requires an active nucleus to account for the emissions, which arise from rapid star formation. About another ISO target Gilmore writes: "In one lovely example, of two intersecting disk galaxies known as the Antennae, it is even possible to resolve the spot where the two disks currently cross, and to see the progression of star formation across the disk as the two galaxies orbit through each other." (Nature, 21 November 1996, p. 211) One year after its launch, ISO is exceptionally popular among astronomers, not only in Europe but worldwide. A panel of American astronomers, reporting to NASA on the scientific merits of eight astrophysical space missions, gives ISO the highest ranking and calls it "the major infrared mission of the decade". At a time when NASA is reducing its budgets for some astrophysical missions, its funding of ISO-related research is increasing. The demand for a share in ISO's unique view of the infrared universe is insatiable. ESA has been overwhelmed by new proposals for observations, coming from 511 groups of astronomers in ESA's own Member States and in the USA and Japan. Although ISO is extremely efficient, performing an average of 45 observations a day, it could not cope with 16,000 observations requested as additions to ISO's already busy programme for 1997. The time allocation committee has had to turn

  13. LRP16 integrates into NF-κB transcriptional complex and is required for its functional activation.

    Directory of Open Access Journals (Sweden)

    Zhiqiang Wu

    Full Text Available BACKGROUND: Nuclear factor κB (NF-κB-mediated pathways have been widely implicated in cell survival, development and tumor progression. Although the molecular events of determining NF-κB translocation from cytoplasm to nucleus have been extensively documented, the regulatory mechanisms of NF-κB activity inside the nucleus are still poorly understood. Being a special member of macro domain proteins, LRP16 was previously identified as a coactivator of both estrogen receptor and androgen receptor, and as an interactor of NF-κB coactivator UXT. Here, we investigated the regulatory role of LRP16 on NF-κB activation. METHODOLOGY: GST pull-down and coimmunoprecipitation (CoIP assays assessed protein-protein interactions. The functional activity of NF-κB was assessed by luciferase assays, changes in expression of its target genes, and its DNA binding ability. Annexin V staining and flow cytometry analysis were used to evaluate cell apoptosis. Immunohistochemical staining of LRP16 and enzyme-linked immunosorbent assay-based evaluation of active NF-κB were performed on primary human gastric carcinoma samples. RESULTS: We demonstrate that LRP16 integrates into NF-κB transcriptional complex through associating with its p65 component. RNA interference knockdown of the endogenous LRP16 in cells leads to impaired NF-κB activity and significantly attenuated NF-κB-dependent gene expression. Mechanistic analysis revealed that knockdown of LRP16 did not affect tumor necrosis factor α (TNF-α-induced nuclear translocation of NF-κB, but blunted the formation or stabilization of functional NF-κB/p300/CREB-binding protein transcription complex in the nucleus. In addition, knockdown of LRP16 also sensitizes cells to apoptosis induced by TNF-α. Finally, a positive link between LRP16 expression intensity in nuclei of tumor cells and NF-κB activity was preliminarily established in human gastric carcinoma specimens. CONCLUSIONS: Our findings not only

  14. Transforming the Land Administration Domain Model (LADM) into an ISO Standard (ISO19152)

    OpenAIRE

    C. H. J. Lemmen; van Oosterom, P.J.M.; Uitermark, H.T.; Thompson, R.J.; Hespanha, J.P.

    2009-01-01

    In February 2008, FIG submitted the Land Administration Domain Model (LADM) to the International Organization for Standardization (ISO). After three meetings with the LADM/ISO19152 Project Team (in Copenhagen, Denmark, May 2008; in Delft, The Netherlands, September 2008; and in Tsukuba, Japan, December 2008) we are now preparing a Committee Draft (CD), the next stage in the development process of an International Standard.

  15. Implementation of ISO 50001 Energy Management System in Sports Stadia

    National Research Council Canada - National Science Library

    Aidan Byrne; Martin Barrett; Richard Kelly

    2014-01-01

    ... Stadium’s recent implementation of the ISO 50001 Energy Management System. The authors identify the potential challenges and benefits of implementing the ISO 50001 Energy Management System in sports stadia...

  16. Integrative Augmentation with the New ISO 10000 Standards1

    Directory of Open Access Journals (Sweden)

    Karapetrovic Stanislav

    2014-11-01

    Full Text Available Application and integration of the systems based on ISO 10001: 2007 and other new augmentative standards are discussed. Particular attention is paid to the quality management standards forming the front end of the ISO 10000 series, namely ISO 10001, ISO 10002 and ISO 10003, as well as ISO 19011, the standard which stems from the original augmentative ISO 10000 guideline, specifically ISO 10011 on quality auditing. The ability of these and other similar standards to augment the performance of quality management systems in organizations and to foster integration of the respective augmentative systems themselves or within the overarching management systems is addressed and further illustrated through an example of their use in engineering education.

  17. RAGE Plays a Role in LPS-Induced NF-κB Activation and Endothelial Hyperpermeability.

    Science.gov (United States)

    Wang, Liqun; Wu, Jie; Guo, Xiaohua; Huang, Xuliang; Huang, Qiaobing

    2017-03-30

    Endothelial functional dysregulation and barrier disruption contribute to the initiation and development of sepsis. The receptor for advanced glycation end products (RAGE) has been demonstrated to be involved in the pathogenesis of sepsis. The present study aimed to investigate the role of RAGE in lipopolysaccharide (LPS)-induced nuclear factor-κB (NF-κB) activation in endothelial cells and the consequent endothelial hyperpermeability. LPS-induced upregulation of RAGE protein expression in human umbilical vein endothelial cells (HUVECs) was detected by western blotting. Activation of NF-κB was revealed using western blotting and immunofluorescent staining. LPS-elicited endothelial hyperpermeability was explored by transendothelial electrical resistance (TER) assay and endothelial monolayer permeability assay. The blocking antibody specific to RAGE was used to confirm the role of RAGE in LPS-mediated NF-κB activation and endothelial barrier disruption. We found that LPS upregulated the protein expression of RAGE in a dose- and time-dependent manner in HUVECs. Moreover, LPS triggered a significant phosphorylation and degradation of IκBα, as well as NF-κB p65 nuclear translocation. Moreover, we observed a significant increase in endothelial permeability after LPS treatment. However, the RAGE blocking antibody attenuated LPS-evoked NF-κB activation and endothelial hyperpermeability. Our results suggest that RAGE plays an important role in LPS-induced NF-κB activation and endothelial barrier dysfunction.

  18. Inhibition of SIRT2 in merlin/NF2-mutant Schwann cells triggers necrosis.

    Science.gov (United States)

    Petrilli, Alejandra; Bott, Marga; Fernández-Valle, Cristina

    2013-12-01

    Mutations in the NF2 gene cause Neurofibromatosis Type 2 (NF2), a disorder characterized by the development of schwannomas, meningiomas and ependymomas in the nervous system. Merlin, a tumor suppressor encoded by the NF2 gene, modulates activity of many essential signaling pathways. Yet despite increasing knowledge of merlin function, there are no NF2 drug therapies. In a pilot high-throughput screen of the Library of Pharmacologically Active Compounds, we assayed for compounds capable of reducing viability of mouse Schwann cells (MSC) with Nf2 inactivation as a cellular model for human NF2 schwannomas. AGK2, a SIRT2 (sirtuin 2) inhibitor, was identified as a candidate compound. SIRT2 is one of seven mammalian sirtuins that are NAD+-dependent protein deacetylases. We show that merlin-mutant MSC have higher expression levels of SIRT2 and lower levels of overall lysine acetylation than wild-type control MSC. Pharmacological inhibition of SIRT2 decreases merlin-mutant MSC viability in a dose dependent manner without substantially reducing wild-type MSC viability. Inhibition of SIRT2 activity in merlin-mutant MSC is accompanied by release of lactate dehydrogenase and high mobility group box 1 protein into the medium in the absence of significant apoptosis, autophagy, or cell cycle arrest. These findings suggest that SIRT2 inhibition triggers necrosis of merlin-mutant MSCs and that SIRT2 is a potential NF2 drug target.

  19. Dimethyl sulfoxide modulates NF-kappa B and cytokine activation in lipopolysaccharide-treated murine macrophages.

    Science.gov (United States)

    Kelly, K A; Hill, M R; Youkhana, K; Wanker, F; Gimble, J M

    1994-01-01

    Antioxidants are protective against septic shock in animal models. Recently, free radical scavengers have been found to inhibit the activation of the NF-kappa B protein in a number of cell lines. This transcriptional regulatory protein binds to the promoters of the proinflammatory cytokines tumor necrosis factor, interleukin-6, and the macrophage inflammatory proteins. The current work examined lipopolysaccharide-induced NF-kappa B activation in the J774 macrophage-like cell line and primary peritoneal macrophages from lipopolysaccharide-responsive (C3HeB/Fej) and -nonresponsive (C3H/HeJ) murine strains. The DNA-binding activity of the NF-kappa B protein directly correlated with mRNA expression for the genes encoding the proinflammatory cytokines and the free radical scavenging enzyme, superoxide dismutase. Both the p50 and p65 NF-kappa B subunits were detected on gel supershift assays. Minimal NF-kappa B activity was observed following exposure of C3H/HeJ macrophages to lipopolysaccharide. The antioxidant dimethyl sulfoxide decreased the level of NF-kappa B activation in the J774 cells. This correlated with decreased expression of cytokine mRNAs and tumor necrosis factor bioactivity. These results suggest that modulation of NF-kappa B activation may provide a mechanism through which antioxidants protect against endotoxemia in murine models. Images PMID:8039880

  20. Binding of NF-κB to nucleosomes: effect of translational positioning, nucleosome remodeling and linker histone H1.

    Directory of Open Access Journals (Sweden)

    Imtiaz Nisar Lone

    Full Text Available NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser footprinting, hydroxyl footprinting and electrophoretic mobility shift assay to investigate the binding of NF-κB to nucleosomal templates. We show that NF-κB p50 homodimer is able to bind to its recognition sequence, when it is localized at the edge of the core particle, but not when the recognition sequence is at the interior of the nucleosome. Remodeling of the nucleosome by the chromatin remodeling machine RSC was not sufficient to allow binding of NF-κB to its recognition sequence located in the vicinity of the nucleosome dyad, but RSC-induced histone octamer sliding allowed clearly detectable binding of NF-κB with the slid particle. Importantly, nucleosome dilution-driven removal of H2A-H2B dimer led to complete accessibility of the site located close to the dyad to NF-κB. Finally, we found that NF-κB was able to displace histone H1 and prevent its binding to nucleosome. These data provide important insight on the role of chromatin structure in the regulation of transcription of NF-κB dependent genes.

  1. Binding of NF-κB to Nucleosomes: Effect of Translational Positioning, Nucleosome Remodeling and Linker Histone H1

    Science.gov (United States)

    Lone, Imtiaz Nisar; Shukla, Manu Shubhdarshan; Charles Richard, John Lalith; Peshev, Zahary Yordanov; Dimitrov, Stefan; Angelov, Dimitar

    2013-01-01

    NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser footprinting, hydroxyl footprinting and electrophoretic mobility shift assay to investigate the binding of NF-κB to nucleosomal templates. We show that NF-κB p50 homodimer is able to bind to its recognition sequence, when it is localized at the edge of the core particle, but not when the recognition sequence is at the interior of the nucleosome. Remodeling of the nucleosome by the chromatin remodeling machine RSC was not sufficient to allow binding of NF-κB to its recognition sequence located in the vicinity of the nucleosome dyad, but RSC-induced histone octamer sliding allowed clearly detectable binding of NF-κB with the slid particle. Importantly, nucleosome dilution-driven removal of H2A–H2B dimer led to complete accessibility of the site located close to the dyad to NF-κB. Finally, we found that NF-κB was able to displace histone H1 and prevent its binding to nucleosome. These data provide important insight on the role of chromatin structure in the regulation of transcription of NF-κB dependent genes. PMID:24086160

  2. Energy Management Systems: from EN 16001 to ISO 50001

    OpenAIRE

    DUGLIO, Stefano

    2011-01-01

    In July 2009, The European Standard EN 16001 on the Energy Management Systems was issued. However, different authors have pointed out certain differences between the European Standard EN 16001 and ISO 50001. The article presents the main differences between the old EN 16001 Standard and the new ISO 50001. It also provides important information on the adoption of ISO 50001 at world level.

  3. An introduction to information security and ISO27001:2013

    CERN Document Server

    Watkins, Steve

    2013-01-01

    Up to date with the latest version of the Standard (ISO27001:2013), An Introduction to information security and ISO27001:2013 is the perfect solution for anyone wanting an accurate, fast, easy-to-read primer on information security from an acknowledged expert on ISO27001.

  4. The Relationship between the Training Function and ISO-9000 Registration.

    Science.gov (United States)

    Maxwell, Randy C.; Jost, Karen L.

    ISO 9000 is one of a series of international standards providing guidelines and governing quality of products and services. The ISO 9000 certification demonstrates the capability of a supplier to control the processes that determine the acceptability of the product or service being supplied. This paper focuses on the training aspects of ISO 9000…

  5. Promoting Strong ISO 50001 Outcomes with Supportive National Infrastructure

    Energy Technology Data Exchange (ETDEWEB)

    McKane, Aimee, T.; Siciliano, Graziella; de los Reyes, Pamela

    2015-08-04

    The ISO 50001 standard is a key mechanism for reducing greenhouse gas emissions and improving energy efficiency globally. An increasing number of companies are seeking certification, creating the need for personnel that are competent to conduct ISO 50001 certification audits. The growth of ISO 50001 is expected to accelerate as more companies integrate ISO 50001 into their corporate sustainability strategies and supplier requirements. Robust implementation of ISO 50001 represents an important tool for countries with climate change mitigation goals. Because of its dual focus on continual improvement of an organization’s energy management system (EnMS) and its energy performance improvement, ISO 50001 requires skills of both implementers and certification auditors that are not well-supported by current credentials and training. This paper describes an effort to address skill gaps of certification auditors, a critical factor to ensure that ISO 50001 implementations are robust and result in continued energy performance improvement. A collaboration of governments through the Energy Management Working Group (EMWG), formerly under Global Superior Energy Performance (GSEP), has formed to build workforce capacity for ISO 50001 certification audits. The EMWG is leading the development of an internationally-relevant certification scheme for ISO 50001 Lead Auditor that meets requirements for ISO/IEC 17024 accreditation and ISO 50003 for defining ISO 50001 Lead Auditor competency. Wider availability of competent ISO 50001 Lead Auditors will ultimately increase the impact and market value of ISO 50001 certification and improve consistency of ISO 50001 certification outcomes by establishing a standardized and high level of knowledge and skills globally.

  6. Incorporating ISO Metadata Using HDF Product Designer

    Science.gov (United States)

    Jelenak, Aleksandar; Kozimor, John; Habermann, Ted

    2016-01-01

    The need to store in HDF5 files increasing amounts of metadata of various complexity is greatly overcoming the capabilities of the Earth science metadata conventions currently in use. Data producers until now did not have much choice but to come up with ad hoc solutions to this challenge. Such solutions, in turn, pose a wide range of issues for data managers, distributors, and, ultimately, data users. The HDF Group is experimenting on a novel approach of using ISO 19115 metadata objects as a catch-all container for all the metadata that cannot be fitted into the current Earth science data conventions. This presentation will showcase how the HDF Product Designer software can be utilized to help data producers include various ISO metadata objects in their products.

  7. Neuronal RING finger protein 11 (RNF11 regulates canonical NF-κB signaling

    Directory of Open Access Journals (Sweden)

    Pranski Elaine L

    2012-04-01

    Full Text Available Abstract Background The RING domain-containing protein RING finger protein 11 (RNF11 is a member of the A20 ubiquitin-editing protein complex and modulates peripheral NF-κB signaling. RNF11 is robustly expressed in neurons and colocalizes with a population of α-synuclein-positive Lewy bodies and neurites in Parkinson disease patients. The NF-κB pathway has an important role in the vertebrate nervous system, where the absence of NF-κB activity during development can result in learning and memory deficits, whereas chronic NF-κB activation is associated with persistent neuroinflammation. We examined the functional role of RNF11 with respect to canonical NF-κB signaling in neurons to gain understanding of the tight association of inflammatory pathways, including NF-κB, with the pathogenesis of neurodegenerative diseases. Methods and results Luciferase assays were employed to assess NF-κB activity under targeted short hairpin RNA (shRNA knockdown of RNF11 in human neuroblastoma cells and murine primary neurons, which suggested that RNF11 acts as a negative regulator of canonical neuronal NF-κB signaling. These results were further supported by analyses of p65 translocation to the nucleus following depletion of RNF11. Coimmunoprecipitation experiments indicated that RNF11 associates with members of the A20 ubiquitin-editing protein complex in neurons. Site-directed mutagenesis of the myristoylation domain, which is necessary for endosomal targeting of RNF11, altered the impact of RNF11 on NF-κB signaling and abrogated RNF11’s association with the A20 ubiquitin-editing protein complex. A partial effect on canonical NF-κB signaling and an association with the A20 ubiquitin-editing protein complex was observed with mutagenesis of the PPxY motif, a proline-rich region involved in Nedd4-like protein interactions. Last, shRNA-mediated reduction of RNF11 in neurons and neuronal cell lines elevated levels of monocyte chemoattractant protein 1 and

  8. ISO 9000: 2000: towards a learning organization ?

    Directory of Open Access Journals (Sweden)

    Michel DOMENC

    2009-01-01

    Full Text Available The 2000 version of ISO 9000 contains major changes compared to the 1994 version. We hypothesize that the changes it induces are in line of research applying the emergence of a new "model" organization. From a field survey among actors involved in implementing the new standard, the research presented examines changes recorded in terms of spaces of communication and cooperation, competence, shared responsibilities. These changes involved the development of a learning organization.

  9. Revision of ISO 15859 Aerospace Fluid Standards

    Science.gov (United States)

    Greene, Benjamin; McClure, Mark B.

    2012-01-01

    A detailed review of ISO 15859 "Space Systems - Fluid Characteristics, Sampling and Test Methods" was performed An approach to revising Parts 1-9 and 11-13 was developed and concurred by the NASA Technical Standards Program Office. The approach was to align them with the highest level source documents, and not to program-specific requirements. The updated documents were prepared and presented.

  10. [Current status of ISO 15189 accreditation system].

    Science.gov (United States)

    Watanabe, Kiyoaki; Kubono, Katsuo; Shimoda, Katsuji

    2012-07-01

    The Japan Accreditation Board (JAB) mainly involves the ISO 15189 accreditation system with support from the Japanese Committee for Clinical Laboratory Standards (JCCLS). The currently available procedure to obtain accreditation is as below. Firstly, it is necessary for applicants to prepare ISO 15189 and related documents in each laboratory. Then a JAB assessor will conduct a preliminary assessment to check if the applicant is ready to be accredited. Subsequently, a team consisting of one to five JAB assessors and/or technical experts will conduct the initial assessment, usually for two days. Finally, the team will make a recommendation to the JAB Accreditation Committee for Medical Laboratory on its evaluation for accreditation. If the Accreditation Committee approves the recommendation of the assessment team, the applicant will be granted accreditation and issued with a certificate of accreditation. According to EU data in February 2011, about 1,300 medical laboratories obtained the ISO 15189 accreditation. The numbers of accredited laboratories are 482 in Germany, 276 in England, 209 in France, 100 in Czechoslovakia etc. Similarly, the data for the Asia-Pacific region in June 2011 showed that the numbers of accredited laboratories are 638 in Australia, 287 in India, 220 in Canada, 160 in Taiwan etc. Although 59 laboratories are accredited in Japan, the ISO 15189 accreditation is not so widespread compared with other countries. It is now expected that the government and/or related bodies will have sufficient understanding of this accreditation system to further its development in Japan. [Rinsho Byori 60: 653-659, 2012

  11. ISO 55000: Creating an asset management system.

    Science.gov (United States)

    Bradley, Chris; Main, Kevin

    2015-02-01

    In the October 2014 issue of HEJ, Keith Hamer, group vice-president, Asset Management & Engineering at Sodexo, and marketing director at Asset Wisdom, Kevin Main, argued that the new ISO 55000 standards present facilities managers with an opportunity to create 'a joined-up, whole lifecycle approach' to managing and delivering value from assets. In this article, Kevin Main and Chris Bradley, who runs various asset management projects, examine the process of creating an asset management system.

  12. ISO TC211 standards on Provenance for Earth science

    Science.gov (United States)

    Di, L.; Deng, M.

    2014-12-01

    Data provenance, also called lineage, records the derivation history of a data product. The history could include the algorithms used, the process steps taken, the computing environment run, data sources input to the processes, the organization/person responsible for the product, etc. Provenance provides important information to data users for them to determine the usability and reliability of the product. In the science domain, the data provenance is especially important since scientists need to use the information to determine the scientific validity of a data product and to decide if such a product can be used as the basis for further scientific analysis. Provenance is a kind of metadata. In Earth science domain, the International Organization for Standardization (ISO) Technical Committee 211 (ISO TC 211) have set geospatial metadata standards for geospatial data, including ISO 19115:2003-Metadata, ISO 19115-2:2009-Metadata-Part 2: Extensions for imagery and gridded data, and ISO 19115-1:2014 - Metadata -- Part 1: Fundamentals. ISO 19115 and ISO 19115-1 define the fundamental metadata for documenting geospatial data products, and ISO 19115-2 provides additional metadata classes for imagery and gridded data. ISO 19115-1:2014 is the revised version of ISO 19115:2003. ISO 19115 and ISO 19115-1 define fundamental lineage information classes and subclasses. They miss some key information classes needed for documenting the provenance in the Web service environment, such as the running environment, the algorithms, and software executables. However, ISO 19115-2 extends the lineage model in ISO 19115 and provides additional metadata classes needed for documenting provenance information. The combination of lineage models in ISO 19115 and ISO 19115-2 provides a comprehensive provenance information model needed for the web service environment. Currently the ISO Provence standard is not compatible with W3C Prov standard. The revision of ISO 19115-2 will be started in

  13. Enzyme assays

    OpenAIRE

    Bisswanger, Hans

    2014-01-01

    The essential requirements for enzyme assays are described and frequently occurring errors and pitfalls as well as their avoidance are discussed. The main factors, which must be considered for assaying enzymes, are temperature, pH, ionic strength and the proper concentrations of the essential components like substrates and enzymes. Standardization of these parameters would be desirable, but the diversity of the features of different enzymes prevents unification of assay conditions. Neverthele...

  14. Biliverdin inhibits activation of NF-kappaB: reversal of inhibition by human biliverdin reductase.

    Science.gov (United States)

    Gibbs, Peter E M; Maines, Mahin D

    2007-12-01

    hBVR functions in the cell as a reductase and as a kinase. In the first capacity, it reduces biliverdin, the product of HO activity, to the effective intracellular antioxidant, bilirubin; as a dual-specificity kinase (S/T/Y) it activates the MAPK and IGF/IRK receptor signal transduction pathways. NF-kappaB and the MAPK pathway are activated by ROS, which results in the activation of stress-inducible genes, including ho-1. Presently, we report on the negative effect of biliverdin on NF-kappaB activation and the converse effect of hBVR. Biliverdin, in a concentration- and time-dependent manner, inhibited transcriptional activity of NF-kappaB in HEK293A cells. Nuclear extracts from biliverdin-treated cells show reduced DNA binding of NF-kappaB in an electromobility shift assay, whereas extracts from cells treated with TNF-alpha showed enhanced binding. Coimmunoprecipitation data show hBVR binds to the 65 kDa subunit of NF-kappaB, and that this is dependent on activation by TNF-alpha. Overexpression of hBVR enhanced both the basal and TNF-alpha-mediated activation of NF-kappaB and also that of the NF-kappaB-activated iNOS gene. Also, overexpression of hBVR arrested the cell cycle in the G(1)/G(0) phase and reduced the number of cells in S phase. Similar results were observed with MCF-7 cells. Because of the Janus nature of NF-kappaB activity in the cell and the inhibitory action of biliverdin, the present findings provide a foundation for therapeutic intervention in inflammatory diseases and cancer that may be attained by preventing reduction of biliverdin. On the other hand, by increasing BVR levels beneficial functions of NF-kappaB might be augmented. (c) 2007 Wiley-Liss, Inc.

  15. Desflurane preconditioning induces oscillation of NF-κB in human umbilical vein endothelial cells.

    Directory of Open Access Journals (Sweden)

    Juan Yi

    Full Text Available BACKGROUND: Nuclear factor kappa B (NF-κB has been implicated in anesthetic preconditioning (APC induced protection against anoxia and reoxygenation (A/R injury. The authors hypothesized that desflurane preconditioning would induce NF-κB oscillation and prevent endothelial cells apoptosis. METHODS: A human umbilical vein endothelial cells (HUVECs A/R injury model was used. A 30 minute desflurane treatment was initiated before anoxia. NF-κB inhibitor BAY11-7082 was administered in some experiments before desflurane preconditioning. Cells apoptosis was analyzed by flow cytometry using annexin V-fluorescein isothiocyanate staining and cell viability was evaluated by modified tertrozalium salt (MTT assay. The cellular superoxide dismutases (SOD activitiy were tested by water-soluble tetrazolium salt (WST-1 assay. NF-κB p65 subunit nuclear translocation was detected by immunofluorescence staining. Expression of inhibitor of NF-κB-α (IκBα, NF-κB p65 and cellular inhibitor of apoptosis 1 (c-IAP1, B-cell leukemia/lymphoma 2 (Bcl-2, cysteine containing aspartate specific protease 3 (caspases-3 and second mitochondrial-derived activator of caspase (SMAC/DIABLO were determined by western blot. RESULTS: Desflurane preconditioning caused phosphorylation and nuclear translocation of NF-κB before anoxia, on the contrary, induced the synthesis of IκBα and inhibition of NF-κB after reoxygenation. Desflurane preconditioning up-regulated the expression of c-IAP1 and Bcl-2, blocked the cleavage of caspase-3 and reduced SMAC release, and decreased the cell death of HUVECs after A/R. The protective effect was abolished by BAY11-7082 administered before desflurane. CONCLUSIONS: The results demonstrated that desflurane activated NF-κB during the preconditioning period and inhibited excessive activation of NF-κB in reperfusion. And the oscillation of NF-κB induced by desflurane preconditioning finally up-regulated antiapoptotic proteins expression and

  16. Ras-Independent Function of NF1

    Science.gov (United States)

    2013-05-01

    months 1-12). Perform immunofluorescence staining on pNF B, CD3 (T-cell), CD4 (helper T cells), CD8 (killer T cells), Gr-1 (granulocytes) and MAC-1...including an increased level of pNF B, increased infiltration of immune cells to the dermis as T-cells, granulocytes and macrophages (figure 4A-D). Moreover...Our staining results proved that loss of NF1 results in increased inflammatory responses only in old mice, including an increased level of pNF B

  17. Lean principles adoption in environmental management system (EMS - ISO 14001

    Directory of Open Access Journals (Sweden)

    Perumal Puvanasvaran

    2012-12-01

    Full Text Available Purpose: The purpose of this study is to examine the characteristic of the lean principles into ISO 14001 and to propose linkage of the lean principles and ISO 14001.Design/methodology/approach: To achieve the objective of the study, literature survey and quantitative research method using questionnaires survey are used.Findings and Originality/value: The findings of this study confirm that ISO 14001 certified company adopted lean production practices.  The study also proves that lean principles have positive and significant relationship with ISO 14001 EMS and the linkage can be made between lean principles and ISO 14001 to achieve Continual Improvement.Research limitations/implications: The small sizes of the sample of the participating companies are the main limitations of this study and this research mainly focuses on the manufacturing environment and services industry.Practical implications: This research show that all ISO 14001 companies do adopt at least one lean production practices and the main findings are lean principles has positive and highly significant relationship with ISO 14001 requirements.  This is because the integration of lean principles into ISO 14001 will serve practical methods for ISO14001 EMS to achieve the continual improvement.Originality/value: This research is amongst the first to study the combined lean principles with ISO 1400.  Based on the current situation, there is no integration within this two management system. 

  18. ISO 639-1 and ISO 639-2: International Standards for Language Codes. ISO 15924: International Standard for Names of Scripts.

    Science.gov (United States)

    Byrum, John D.

    This paper describes two international standards for the representation of the names of languages. The first (ISO 639-1), published in 1988, provides two-letter codes for 136 languages and was produced primarily to meet terminological needs. The second (ISO 639-2) appeared in late 1998 and includes three-letter codes for 460 languages. This list…

  19. Plasma neurofilament pNF-H concentration is not increased in acute equine grass sickness.

    Science.gov (United States)

    Stratford, C H; Pemberton, A; Cameron, L; McGorum, B C

    2013-03-01

    Although a presumptive diagnosis of acute grass sickness (AGS) can be made on the basis of clinical signs, a definitive ante mortem diagnosis currently requires histological examination of enteric ganglia. Development of an accurate noninvasive ante mortem diagnostic test is therefore warranted. The objective of this study was to determine whether quantification of the plasma concentrations of the heavily phosphorylated form of major neurofilament subunit NF-H (pNF-H), which mirror the degree of axonal degeneration in some human and animal neurodegenerative disorders, could distinguish AGS-affected and control horses. The pNF-H was quantified in plasma from 20 AGS cases and 20 control horses using a commercial enzyme-linked immunosorbent assay kit. Five AGS and 4 control samples had detectable pNF-H concentrations (>0.0759 ng/ml). There was no significant intergroup difference in pNF-H concentrations. It was concluded that plasma pNF-H is not a useful biomarker for the diagnosis of AGS. © 2012 EVJ Ltd.

  20. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury.

    Science.gov (United States)

    Anderson, Kevin J; Scheff, Stephen W; Miller, Kelly M; Roberts, Kelly N; Gilmer, Lesley K; Yang, Cui; Shaw, Gerry

    2008-09-01

    The detection of neuron-specific proteins in blood might allow quantification of the degree of neuropathology in experimental and clinical contexts. We have been studying a novel blood biomarker of axonal injury, the heavily phosphorylated axonal form of the high molecular weight neurofilament subunit NF-H (pNF-H). We hypothesized that this protein would be released from damaged and degenerating neurons following experimental traumatic brain injury (TBI) in amounts large enough to allow its detection in blood and that the levels detected would reflect the degree of injury severity. An enzyme-linked immunosorbent assay (ELISA) capture assay capable of detecting nanogram amounts of pNF-H was used to test blood of rats subjected to experimental TBI using a controlled cortical impact (CCI) device. Animals were subjected to a mild (1.0 mm), moderate (1.5 mm), or severe (2.0 mm) cortical contusion, and blood samples were taken at defined times post-injury. The assay detected the presence of pNF-H as early as 6 h post-injury; levels peaked at 24-48 h, and then slowly decreased to baseline over several days post-injury. No signal above baseline was detectable in control animals. Analysis of variance (ANOVA) showed a significant effect of lesion severity, and post hoc analysis revealed that animals given a moderate and severe contusion showed higher levels of blood pNF-H than controls. In addition, the peak levels of pNF-H detected at both 24 and 48 h post-injury correlated with the degree of injury as determined by volumetric analysis of spared cortical tissue. Relative amounts of pNF-H were also determined in different areas of the central nervous system (CNS) and were found to be highest in regions containing large-diameter axons, including spinal cord and brainstem, and lowest in the cerebral cortex and hippocampus. These findings suggest that the measurement of blood levels of pNF-H is a convenient method for assessing neuropathology following TBI.

  1. Norepinephrine activates NF-κB transcription factor in cultured rat pineal gland.

    Science.gov (United States)

    Villela, Darine; de Sá Lima, Larissa; Peres, Rafael; Peliciari-Garcia, Rodrigo Antonio; do Amaral, Fernanda Gaspar; Cipolla-Neto, José; Scavone, Cristóforo; Afeche, Solange Castro

    2014-01-17

    The circadian rhythm in mammalian pineal melatonin secretion is modulated by norepinephrine (NE) released at night. NE interaction with β1-adrenoceptors activates PKA that phosphorylates the transcription factor CREB, leading to the transcription and translation of the arylalkylamine-N-acetyltransferase (AANAT) enzyme. Several studies have reported the interplay between CREB and the nuclear factor-κB (NF-κB) and a circadian rhythm for this transcription factor was recently described in the rat pineal gland. In this work we studied a direct effect of NE on NF-κB activation and the role played by this factor on melatonin synthesis and Aanat transcription and activity. Cultured rat pineal glands were incubated in the presence of two different NF-κB inhibitors, pyrrolidine-dithiocarbamate or sodium salicylate, and stimulated with NE. Melatonin content was quantified by HPLC with electrochemical detection. AANAT activity was measured by a radiometric assay and the expression of Aanat mRNA was analyzed by real-time PCR. Gel shift assay was performed to study the NF-κB activation in cultured rat pineal glands stimulated by NE. Our results showed that the p50/p50 homodimer of NF-κB is activated by NE and that it has a role in melatonin synthesis, acting on Aanat transcription and activity. Here we present evidence that NF-κB is an important transcription factor that acts, directly or indirectly, on Aanat transcription and activity leading to a modulation of melatonin synthesis. NE plays a role in the translocation of NF-κB p50/p50 homodimer to the nucleus of pinealocytes, thus probably influencing the nocturnal pineal melatonin synthesis. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Raf Kinase Inhibitor Protein Interacts with NF-κB-Inducing Kinase and TAK1 and Inhibits NF-κB Activation

    Science.gov (United States)

    Yeung, Kam C.; Rose, David W.; Dhillon, Amardeep S.; Yaros, Diane; Gustafsson, Marcus; Chatterjee, Devasis; McFerran, Brian; Wyche, James; Kolch, Walter; Sedivy, John M.

    2001-01-01

    The Raf kinase inhibitor protein (RKIP) acts as a negative regulator of the mitogen-activated protein (MAP) kinase (MAPK) cascade initiated by Raf-1. RKIP inhibits the phosphorylation of MAP/extracellular signal-regulated kinase 1 (MEK1) by Raf-1 by disrupting the interaction between these two kinases. We show here that RKIP also antagonizes the signal transduction pathways that mediate the activation of the transcription factor nuclear factor kappa B (NF-κB) in response to stimulation with tumor necrosis factor alpha (TNF-α) or interleukin 1 beta. Modulation of RKIP expression levels affected NF-κB signaling independent of the MAPK pathway. Genetic epistasis analysis involving the ectopic expression of kinases acting in the NF-κB pathway indicated that RKIP acts upstream of the kinase complex that mediates the phosphorylation and inactivation of the inhibitor of NF-κB (IκB). In vitro kinase assays showed that RKIP antagonizes the activation of the IκB kinase (IKK) activity elicited by TNF-α. RKIP physically interacted with four kinases of the NF-κB activation pathway, NF-κB-inducing kinase, transforming growth factor beta-activated kinase 1, IKKα, and IKKβ. This mode of action bears striking similarities to the interactions of RKIP with Raf-1 and MEK1 in the MAPK pathway. Emerging data from diverse organisms suggest that RKIP and RKIP-related proteins represent a new and evolutionarily highly conserved family of protein kinase regulators. Since the MAPK and NF-κB pathways have physiologically distinct roles, the function of RKIP may be, in part, to coordinate the regulation of these pathways. PMID:11585904

  3. Targeting the NF-κB Pathway as a Combination Therapy for Advanced Thyroid Cancer.

    Directory of Open Access Journals (Sweden)

    Nikita Pozdeyev

    Full Text Available NF-κB signaling plays an important role in tumor cell proliferation, cell survival, angiogenesis, invasion, metastasis and drug/radiation resistance. Combination therapy involving NF-κB pathway inhibition is an attractive strategy for the treatment of advanced forms of thyroid cancer. This study was designed to test the efficacy of NF-κB pathway inhibition in combination with cytotoxic chemotherapy, using docetaxel and ionizing radiation in in vitro models of thyroid cancer. We found that while both docetaxel and ionizing radiation activated NF-κB signaling in thyroid cancer cells, there was no synergistic effect on cell proliferation and/or programmed cell death with either genetic (transduction of a dominant negative mutant form of IκBα or pharmacologic (proteasome inhibitor bortezomib and IKKβ inhibitor GO-Y030 inhibition of the NF-κB pathway in thyroid cancer cell lines BCPAP, 8505C, THJ16T and SW1736. Docetaxel plus bortezomib synergistically decreased in vitro invasion of 8505C cells, but not in the other cell lines. Screening of a panel of clinically relevant targeted therapies for synergy with genetic NF-κB inhibition in a proliferation/cytotoxicity assay identified the histone deacetylase (HDAC inhibitor suberoylanilide hydroxamic acid (SAHA as a potential candidate. However, the synergistic effect was confirmed only in the BCPAP cells. These results indicate that NF-κB inhibitors are unlikely to be beneficial as combination therapy with taxane cytotoxic chemotherapy, external radiation therapy or radioiodine therapy. There may be unique circumstances where NF-κB inhibitors may be considered in combination with docetaxel to reduce tumor invasion or in combination with HDAC inhibitors to reduce tumor growth, but this does not appear to be a combination therapy that could be broadly applied to patients with advanced thyroid cancer. Further research may identify which subsets of patients/tumors may respond to this therapeutic

  4. ISO 9001 PERFORMANCE: A HOLISTIC AND MIXED-METHOD ANALYSIS

    OpenAIRE

    Alcina A. de Sena Portugal Dias*, Iñaki Heras Saizarbitoria

    2016-01-01

    Purpose – The aim of this paper is to analyse the impact of the ISO 9001 Standard on organizational performance from a holistic theoretical perspective, using a mixed-method analysis. More specifically, the qualitative study considers the impact of the adoption of ISO 9001 on the general performance of certified companies. The quantitative study analysed the effect of ISO 9001 on productivity, business value and increase on sales. Design/methodology/approach – A holistic and integra...

  5. Cotation de fabrication avec les normes ISO

    CERN Document Server

    Anselmetti, Bernard

    2010-01-01

    La cotation ISO en production impose de nouvelles méthodologies pour élaborer les documents de maîtrise de la qualité géométrique des produits : les dessins de phase, du brut et d'états intermédiaires pour la conformité des pièces et les fiches de production, de réglage, de contrôle et de surveillance pour la conformité des moyens de production. Une méthode de transfert unidirectionnelle simple permet d'établir des dessins de phase en normes ISO et d'optimiser la répartition des tolérances à isocapabilité. Cette cotation est déclinée en cotation d'état intermédiaire, de production et de réglage. Une méthode tridimensionnelle est proposée pour prendre en compte les effets des défauts angulaires. Une maquette montre les fonctionnalités envisageables pour les futurs outils d'aides au tolérancement de fabrication au sein de la CFAO. De nombreux exercices illustrent les différentes démarches. Ce Manuel de tolérancement est principalement destiné aux préparateurs méthodes et aux c...

  6. Iso-suillin from Suillus flavus Induces Apoptosis in Human Small Cell Lung Cancer H446 Cell Line.

    Science.gov (United States)

    Zhao, Jun-Xia; Zhang, Qing-Shuang; Chen, Ying; Yao, Sheng-Jie; Yan, Yong-Xin; Wang, Ying; Zhang, Jin-Xiu; Wang, Li-An

    2016-05-20

    The suillin isoform iso-suillin is a natural substance isolated from a petroleum ether extract of the fruiting bodies of the mushroom Suillus flavus. Previous studies have found its inhibition effect on some cancer cells, and we aimed to study its effects on human small cell lung cancer H446 cell line. Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Cellular morphological changes (apoptosis and necrosis) were evaluated using an electron microscope and Hoechst 33258 staining detected by the inverted microscope. Flow cytometry was used to detect cell apoptosis, cell cycle distribution, and mitochondrial membrane potential. Protein expression was determined by Western blotting analysis. Here, we describe the ability of iso-suillin to inhibit the growth of H446 cells in time- and dose-dependent way. Iso-suillin had no obvious impact on normal human lymphocyte proliferation at low concentrations (9.09, 18.17, or 36.35 μmol/L) but promoted lymphocyte proliferation at a high concentration (72.70 μmol/L). After treatment of different concentrations of iso-suillin (6.82, 13.63, or 20.45 μmol/L), the apoptosis rate of H446 cells increased with increasing concentrations of iso-suillin (16.70%, 35.54%, and 49.20%, respectively, all P iso-suillin could induce H446 cell apoptosis through the mitochondrial pathway and the death-receptor pathway. Therefore, iso-suillin might have a potential application as a novel drug for lung cancer treatment.

  7. Risk in Management Systems according to ISO standard

    Directory of Open Access Journals (Sweden)

    P. Królas

    2010-07-01

    Full Text Available The purpose of this article was to present selected management standards in context of risk management. It presents main ISOmanagement standards (ISO 9001, ISO 14001, OHSAS 18001, ISO 27001, BS 25999, ISO 31000 that apply to polish enterprises. In thefirst part of this article there are analyzed management standards regarding quality, environment, occupational health and safety,information security, as well as business continuity management and risk management. The second part of the article discusses the processof dealing with risk based on chosen management standard.

  8. ISO standartų pritaikomumas kaimo turizmo versle

    OpenAIRE

    Bričkutė, Ernesta

    2014-01-01

    Lietuvos kaimo turizmo asociacija klasifikuoja kaimo turizmo sodybas, suteikdama joms pagal nustatytus standartus nuo 1 iki 5 gandrų ženklus, tačiau ISO standartai netaikomi kaimo turizmo sodybų kokybės vertinime, todėl kyla probleminis klausimas, ar ISO standartai yra patrauklūs Lietuvos kaimo turizmo apgyvendinimo sektoriui? Tyrimo tikslas – išsiaiškinti ISO standartų pritaikomumą Lietuvos kaimo turizmo versle. Tyrimo uždaviniai: 1. Išanalizuoti ISO standartų sampratą. 2. Atlikt...

  9. The dark side of ISO 14001: The symbolic environmental behavior

    Directory of Open Access Journals (Sweden)

    Vera Ferrón Vílchez

    2017-01-01

    Full Text Available Some of the academic research on ISO 14001 has focused on analyzing the benefits of its adoption. However, this international standard has also received some criticism, particularly in respect of the adoption of ISO 14001 when not accompanied by significant improvements in environmental performance. This study analyzes the relationship between the symbolic environmental behavior and the adoption of ISO 14001. In so doing, it uses binary logistic regression to analyze an international sample of 1961 manufacturing facilities that each employs more than 50 people. The results indicate that the higher the symbolic environmental performance of the firm, the greater the probability of adopting ISO 14001.

  10. Neurofibromatosis type 2 tumor suppressor protein, NF2, induces proteasome-mediated degradation of JC virus T-antigen in human glioblastoma.

    Directory of Open Access Journals (Sweden)

    Sarah Beltrami

    Full Text Available Neurofibromatosis type 2 protein (NF2 has been shown to act as tumor suppressor primarily through its functions as a cytoskeletal scaffold. However, NF2 can also be found in the nucleus, where its role is less clear. Previously, our group has identified JC virus (JCV tumor antigen (T-antigen as a nuclear binding partner for NF2 in tumors derived from JCV T-antigen transgenic mice. The association of NF2 with T-antigen in neuronal origin tumors suggests a potential role for NF2 in regulating the expression of the JCV T-antigen. Here, we report that NF2 suppresses T-antigen protein expression in U-87 MG human glioblastoma cells, which subsequently reduces T-antigen-mediated regulation of the JCV promoter. When T-antigen mRNA was quantified, it was determined that increasing expression of NF2 correlated with an accumulation of T-antigen mRNA; however, a decrease in T-antigen at the protein level was observed. NF2 was found to promote degradation of ubiquitin bound T-antigen protein via a proteasome dependent pathway concomitant with the accumulation of the JCV early mRNA encoding T-antigen. The interaction between T-antigen and NF2 maps to the FERM domain of NF2, which has been shown previously to be responsible for its tumor suppressor activity. Co-immunoprecipitation assays revealed a ternary complex among NF2, T-antigen, and the tumor suppressor protein, p53 within a glioblastoma cell line. Further, these proteins were detected in various degrees in patient tumor tissue, suggesting that these associations may occur in vivo. Collectively, these results demonstrate that NF2 negatively regulates JCV T-antigen expression by proteasome-mediated degradation, and suggest a novel role for NF2 as a suppressor of JCV T-antigen-induced cell cycle regulation.

  11. Analysis of ISO NE Balancing Requirements: Uncertainty-based Secure Ranges for ISO New England Dynamic Inerchange Adjustments

    Energy Technology Data Exchange (ETDEWEB)

    Etingov, Pavel V.; Makarov, Yuri V.; Wu, Di; Hou, Zhangshuan; Sun, Yannan; Maslennikov, S.; Luo, X.; Zheng, T.; George, S.; Knowland, T.; Litvinov, E.; Weaver, S.; Sanchez, E.

    2013-01-31

    The document describes detailed uncertainty quantification (UQ) methodology developed by PNNL to estimate secure ranges of potential dynamic intra-hour interchange adjustments in the ISO-NE system and provides description of the dynamic interchange adjustment (DINA) tool developed under the same contract. The overall system ramping up and down capability, spinning reserve requirements, interchange schedules, load variations and uncertainties from various sources that are relevant to the ISO-NE system are incorporated into the methodology and the tool. The DINA tool has been tested by PNNL and ISO-NE staff engineers using ISO-NE data.

  12. The Nf-actin gene is an important factor for food-cup formation and cytotoxicity of pathogenic Naegleria fowleri.

    Science.gov (United States)

    Sohn, Hae-Jin; Kim, Jong-Hyun; Shin, Myeong-Heon; Song, Kyoung-Ju; Shin, Ho-Joon

    2010-03-01

    Naegleria fowleri destroys target cells by trogocytosis, a phagocytosis mechanism, and a process of piecemeal ingestion of target cells by food-cups. Phagocytosis is an actin-dependent process that involves polymerization of monomeric G-actin into filamentous F-actin. However, despite the numerous studies concerning phagocytosis, its role in the N. fowleri food-cup formation related with trogocytosis has been poorly reported. In this study, we cloned and characterized an Nf-actin gene to elucidate the role of Nf-actin gene in N. fowleri pathogenesis. The Nf-actin gene is composed of 1,128-bp and produced a 54.1-kDa recombinant protein (Nf-actin). The sequence identity was 82% with nonpathogenic Naegleria gruberi but has no sequence identity with other mammals or human actin gene. Anti-Nf-actin polyclonal antibody was produced in BALB/c mice immunized with recombinant Nf-actin. The Nf-actin was localized on the cytoplasm, pseudopodia, and especially, food-cup structure (amoebastome) in N. fowleri trophozoites using immunofluorescence assay. When N. fowleri co-cultured with Chinese hamster ovary cells, Nf-actin was observed to localize around on phagocytic food-cups. We also observed that N. fowleri treated with cytochalasin D as actin polymerization inhibitor or transfected with antisense oligomer of Nf-actin gene had shown the reduced ability of food-cup formation and in vitro cytotoxicity. Finally, it suggests that Nf-actin plays an important role in phagocytic activity of pathogenic N. fowleri.

  13. Intermediate species measurement during iso-butanol auto-ignition

    KAUST Repository

    Ji, Weiqi

    2015-10-01

    © 2015 The Combustion Institute.Published by Elsevier Inc. All rights reserved. This work presents the time histories of intermediate species during the auto-ignition of iso-butanol at high pressure and intermediate temperature conditions obtained using a rapid compression machine and recently developed fast sampling system. Iso-butanol ignition delays were acquired for iso-butanol/O2 mixture with an inert/O2 ratio of 7.26, equivalence ratio of 0.4, in the temperature range of 840-950 K and at pressure of 25 bar. Fast sampling and gas chromatography were used to acquire and quantify the intermediate species during the ignition delay of the same mixture at P = 25.3 bar and T = 905 K. The ignition delay times and quantitative measurements of the mole fraction time histories of methane, ethene, propene, iso-butene, iso-butyraldehyde, iso-butanol, and carbon monoxide were compared with predictions from the detailed mechanisms developed by Sarathy et al., Merchant et al., and Cai et al. It is shown that while the Sarathy mechanism well predicts the overall ignition delay time, it overpredicts ethene by a factor of 6-10, underpredicts iso-butene by a factor of 2, and overpredicts iso-butyraldehyde by a factor of 2. Reaction path and sensitivity analyses were carried out to identify the reactions responsible for the observed inadequacy. The rates of iso-butanol hydrogen atom abstraction by OH radical and the beta-scission reactions of hydroxybutyl radicals were updated based on recently published quantum calculation results. Significant improvements were achieved in predicting ignition delay at high pressures (25 and 30 bar) and the species concentrations of ethene and iso-butene. However, the updated mechanism still overpredicts iso-butyraldehyde concentrations. Also, the updated mechanism degrades the prediction in ignition delay at lower pressure (15 bar) compared to the original mechanism developed by Sarathy et al.

  14. Iso 19011:2002--a combined auditing standard for quality and environmental management systems.

    Science.gov (United States)

    Johnson, G L

    2000-01-01

    In a precedent-setting decision in 1998, the International Organization for Standardization (ISO) directed ISO Technical Committee (TC) 176 on Quality Management and ISO TC 207 on Environmental Management to develop jointly a single guideline standard for auditing quality and environmental management systems. When approved, this standard would replace ISO 10011-1, ISO 10011-2, and ISO 10011-3 on quality auditing and ISO 14010, ISO 14011, and ISO 14012 on environmental auditing. A Joint Working Group (JWG) was established comprising experts from both TC 176 and TC 207 to develop the new standard, ISO 19011, Guidelines on Quality and/or Environmental Management Systems Auditing, and to incorporate lessons learned from efforts to improve compatibility between ISO 9001/9004 and ISO 14001/14004, the standards for quality and environmental management systems, respectively. Work is proceeding on the development of ISO 19011 with an expected completion in early 2002.

  15. Mednarodni standardi - veličine in enote (ISO 31-0 do 31-13)

    OpenAIRE

    Glavič, Peter

    2015-01-01

    In this paper the international standards ISO 31 (Quantities and units) are presented with the following parts: ISO 31-0 (General principles), ISO 31-1 (Space and time), ISO 31-2 (Periodic and related phenomena), ISO 31-3 (Mechanics), ISO 31-4 (Heat), ISO 31-5 (Electricity and magnetism), ISO 31-8 (Physical chemistry and molecular physics), ISO 31-12 (Characteristic numbers)and others. The emphasis is given on the basic principles, which is important for writing of reports, presentations, art...

  16. ANKRD1 modulates inflammatory responses in C2C12 myoblasts through feedback inhibition of NF-κB signaling activity

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xin-Hua [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Bauman, William A. [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Cardozo, Christopher, E-mail: chris.cardozo@va.gov [National Center of Excellence for the Medical Consequences of Spinal Cord Injury, James J. Peter VA Medical Center, Bronx, NY 10468 (United States); Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Rehabilitation Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States); Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY 10029 (United States)

    2015-08-14

    Transcription factors of the nuclear factor-kappa B (NF-κB) family play a pivotal role in inflammation, immunity and cell survival responses. Recent studies revealed that NF-κB also regulates the processes of muscle atrophy. NF-κB activity is regulated by various factors, including ankyrin repeat domain 2 (AnkrD2), which belongs to the muscle ankyrin repeat protein family. Another member of this family, AnkrD1 is also a transcriptional effector. The expression levels of AnkrD1 are highly upregulated in denervated skeletal muscle, suggesting an involvement of AnkrD1 in NF-κB mediated cellular responses to paralysis. However, the molecular mechanism underlying the interactive role of AnkrD1 in NF-κB mediated cellular responses is not well understood. In the current study, we examined the effect of AnkrD1 on NF-κB activity and determined the interactions between AnkrD1 expression and NF-κB signaling induced by TNFα in differentiating C2C12 myoblasts. TNFα upregulated AnkrD1 mRNA and protein levels. AnkrD1-siRNA significantly increased TNFα-induced transcriptional activation of NF-κB, whereas overexpression of AnkrD1 inhibited TNFα-induced NF-κB activity. Co-immunoprecipitation studies demonstrated that AnkrD1 was able to bind p50 subunit of NF-κB and vice versa. Finally, CHIP assays revealed that AnkrD1 bound chromatin at a NF-κB binding site in the AnrkD2 promoter and required NF-κB to do so. These results provide evidence of signaling integration between AnkrD1 and NF-κB pathways, and suggest a novel anti-inflammatory role of AnkrD1 through feedback inhibition of NF-κB transcriptional activity by which AnkrD1 modulates the balance between physiological and pathological inflammatory responses in skeletal muscle. - Highlights: • AnkrD1 is upregulated by TNFα and represses NF-κB-induced transcriptional activity. • AnkrD1 binds to p50 subunit of NF-κB and is recruited to NF-κB bound to chromatin. • AnkrD1 mediates a feed-back inhibitory loop

  17. Manufacturing Bms/Iso System Review

    Science.gov (United States)

    Gomez, Yazmin

    2004-01-01

    The Quality Management System (QMS) is one that recognizes the need to continuously change and improve an organization s products and services as determined by system feedback, and corresponding management decisions. The purpose of a Quality Management System is to minimize quality variability of an organization's products and services. The optimal Quality Management System balances the need for an organization to maintain flexibility in the products and services it provides with the need for providing the appropriate level of discipline and control over the processes used to provide them. The goal of a Quality Management System is to ensure the quality of the products and services while consistently (through minimizing quality variability) meeting or exceeding customer expectations. The GRC Business Management System (BMS) is the foundation of the Center's ISO 9001:2000 registered quality system. ISO 9001 is a quality system model developed by the International Organization for Standardization. BMS supports and promote the Glenn Research Center Quality Policy and wants to ensure the customer satisfaction while also meeting quality standards. My assignment during this summer is to examine the manufacturing processes used to develop research hardware, which in most cases are one of a kind hardware, made with non conventional equipment and materials. During this process of observation I will make a determination, based on my observations of the hardware development processes the best way to meet customer requirements and at the same time achieve the GRC quality standards. The purpose of my task is to review the manufacturing processes identifying opportunities in which to optimize the efficiency of the processes and establish a plan for implementation and continuous improvement.

  18. Effect of natural and semisynthetic pseudoguianolides on the stability of NF-κB:DNA complex studied by agarose gel electrophoresis.

    Science.gov (United States)

    Villagomez, Rodrigo; Hatti-Kaul, Rajni; Sterner, Olov; Almanza, Giovanna; Linares-Pastén, Javier A

    2015-01-01

    The nuclear factor κB (NF-κB) is a promising target for drug discovery. NF-κB is a heterodimeric complex of RelA and p50 subunits that interact with the DNA, regulating the expression of several genes; its dysregulation can trigger diverse diseases including inflammation, immunodeficiency, and cancer. There is some experimental evidence, based on whole cells studies, that natural sesquiterpene lactones (Sls) can inhibit the interaction of NF-κB with DNA, by alkylating the RelA subunit via a Michael addition. In the present work, 28 natural and semisynthetic pseudoguianolides were screened as potential inhibitors of NF-κB in a biochemical assay that was designed using pure NF-κB heterodimer, pseudoguianolides and a ~1000 bp palindromic DNA fragment harboring two NF-κB recognition sequences. By comparing the relative amount of free DNA fragment to the NF-κB - DNA complex, in a routine agarose gel electrophoresis, the destabilizing effect of a compound on the complex is estimated. The results of the assay and the following structure-activity relationship study, allowed the identification of several relevant structural features in the pseudoguaianolide skeleton, which are necessary to enhance the dissociating capacity of NF-κB-DNA complex. The most active compounds are substituted at C-3 (α-carbonyl), in addition to having the α-methylene-γ-lactone moiety which is essential for the alkylation of RelA.

  19. Effect of natural and semisynthetic pseudoguianolides on the stability of NF-κB:DNA complex studied by agarose gel electrophoresis.

    Directory of Open Access Journals (Sweden)

    Rodrigo Villagomez

    Full Text Available The nuclear factor κB (NF-κB is a promising target for drug discovery. NF-κB is a heterodimeric complex of RelA and p50 subunits that interact with the DNA, regulating the expression of several genes; its dysregulation can trigger diverse diseases including inflammation, immunodeficiency, and cancer. There is some experimental evidence, based on whole cells studies, that natural sesquiterpene lactones (Sls can inhibit the interaction of NF-κB with DNA, by alkylating the RelA subunit via a Michael addition. In the present work, 28 natural and semisynthetic pseudoguianolides were screened as potential inhibitors of NF-κB in a biochemical assay that was designed using pure NF-κB heterodimer, pseudoguianolides and a ~1000 bp palindromic DNA fragment harboring two NF-κB recognition sequences. By comparing the relative amount of free DNA fragment to the NF-κB - DNA complex, in a routine agarose gel electrophoresis, the destabilizing effect of a compound on the complex is estimated. The results of the assay and the following structure-activity relationship study, allowed the identification of several relevant structural features in the pseudoguaianolide skeleton, which are necessary to enhance the dissociating capacity of NF-κB-DNA complex. The most active compounds are substituted at C-3 (α-carbonyl, in addition to having the α-methylene-γ-lactone moiety which is essential for the alkylation of RelA.

  20. A Stepwise ISO-Based TQM Implementation Approach Using ISO 9001:2015

    Directory of Open Access Journals (Sweden)

    Chen Chi-kuang

    2016-12-01

    Full Text Available The lack of an implementation roadmap always deters enterprises from choosing Total Quality Management (TQM as its major management approach. This paper proposes a stepwise ISO-based TQM implementation approach which is based on the notion of the new three-dimensional overall business excellence framework developed by Dahlgaard et al. [1]. The proposed approach consists of nine steps comprising three categories: “TQM faith building”, “TQM tools and techniques learning”, and “system development”. The steps in each of the three categories are arranged to span across the proposed nine-step approach. The ISO 9001:2015 standard is used as a case study to demonstrate the proposed approach. The ideas and benefits of the proposed approach are further discussed in relation to this illustration.

  1. Hoogvliet haalt ISO 50001 dankzij TB-student

    NARCIS (Netherlands)

    ir.ing Ruud Thelosen

    2016-01-01

    Derde Jaars stagiaire Technische Bedrijfskunde Werner Hofmeester heeft voor detailgroothandel en distributiecentrum Hoogvliet uit Alphen aan de Rijn de voorbereidingen getroffen en metingen uitgevoerd om de ISO-certificering binnen te halen. Dat is ook binnen 5 maanden gelukt. ISO 50001 gaat over

  2. ISO 14000 - Condition of Competition Romanian Firm on Foreign Market

    OpenAIRE

    Enea, Constanta

    2008-01-01

    Every organization determined to embrace ISO 14000 should have a supporting policy manual, procedures, data collection forms, etc. ISO 14000 is actually a series of international standards on environmental management. It provides a framework for the development of both the system and the supporting audit program.

  3. The Strengths and Weaknesses of ISO 9000 in Vocational Education

    Science.gov (United States)

    Bevans-Gonzales, Theresa L.; Nair, Ajay T.

    2004-01-01

    ISO 9000 is a set of quality standards that assists an organization to identify, correct and prevent errors, and to promote continual improvement. Educational institutions worldwide are implementing ISO 9000 as they face increasing external pressure to maintain accountability for funding. Similar to other countries, in the United States vocational…

  4. Quality Systems and ISO 9000 in Higher Education.

    Science.gov (United States)

    Lundquist, Robert

    1997-01-01

    Examines quality assurance systems in higher education in general and of ISO 9000, a set of international quality standards, in particular. Reports on a survey of 23 institutions worldwide concerning implementation of quality systems and application of the ISO 9000 standards. Concludes that while institutions have found the quality assurance…

  5. ISO 9000 Series Certification Over Time: what have we learnt?

    NARCIS (Netherlands)

    A. van der Wiele (Ton); A.M. Brown (Alan)

    2002-01-01

    textabstractThe ISO 9000 experiences of the same sample of organisations over a five year time period is examined in this paper. The responses to a questionnaire sent out at the end of 1999 to companies which had a reasonably long term experience with the ISO 9000 series quality system are analysed.

  6. High standards: ISO 9000 comes to health care.

    Science.gov (United States)

    Moore, L M

    1999-02-01

    ISO 9000, an international quality system, is catching on in health care. Providers see it as a much less expensive alternative or as a complement to the JCAHO. And because ISO has been used for years in manufacturing, it has one big advantage over JCAHO: employers know it and trust it.

  7. ISO 9000:2000 revisions and their implications.

    Science.gov (United States)

    Sim, P

    2000-05-01

    The planned revision of the ISO 9000 series of standards means that all medical device companies will have to revise their quality systems to align them with the new ISO 9000:2000 standards. This overview outlines the changes and the factors companies need to consider, and highlights future standards activity.

  8. ISO 9000 in het onderwijs: vriend of vijand?

    NARCIS (Netherlands)

    PhD Everard van Kemenade

    2008-01-01

    In dit artikel wordt onderzoek over de effecten van ISO van de laatste twintig jaar op een rij gezet. Het is daarbij belangrijk onderscheid te maken tussen de verschillende versies van ISO 1987, 1994 of de meest recente 2000. Het betreft veel case studies met een beperkt bereik of artikelen op basis

  9. Analysis of ISO 26262 Compliant Techniques for the Automotive Domain

    NARCIS (Netherlands)

    M. S. Kannan; Y. Dajsuren (Yanjindulam); Y. Luo; I. Barosan

    2015-01-01

    htmlabstractThe ISO 26262 standard denes functional safety for automotive E/E systems. Since the publication of the rst edition of this standard in 2011, many dierent safety techniques complying to the ISO 26262 have been developed. However, it is not clear which parts and (sub-) phases of the

  10. ISO 14001 ENVIRONMENTL MANAGEMENT SYSTEMS IN GOVERNMENT AND INDUSTRY

    Science.gov (United States)

    Two new USEPA documents address environmental management systems (EMS) from the perspectives of government and industry and are offered as tools for understanding the process of the ISO 14000 Standards development and usefulness of an EMS approach. The first document, ISO 14001 -...

  11. Evaluating Education Quality in Terms of ISO9000 Standards

    Science.gov (United States)

    Fengchun, Cheng; Vogel, Linda R.; Zhaoyu, Zheng

    2014-01-01

    While nations measure the quality of educational systems by different standards, many nations, such as China, have adopted the ISO9000 quality standards to assess the quality of schools. This article explores the various elements of the the ISO9000 standards and how each element relates to the various groups that are consumers or stakeholders in…

  12. Thoughts on ISO and the development of terminologies in Southern ...

    African Journals Online (AJOL)

    A South African national committee of ISO's Technical Committee 37 was established during 1990. This national committee is the link between South African bodies who develop and standardise terminologies and ISO committees who standardise terminologies with a view to the quality control of products. In this article the ...

  13. ISO 9000 and quality standards improvement in the maintenance ...

    African Journals Online (AJOL)

    ... only relevant but very important to enhance business performance and competiveness of manufacturing firms. The study therefore recommends more firms to adopt ISO 9000 for firms` performance and competiveness. Keywords: ISO 9000, quality management system, maintenance department, certification, performance ...

  14. ISO 9001 in a neonatal intensive care unit (NICU).

    Science.gov (United States)

    Vitner, Gad; Nadir, Erez; Feldman, Michael; Yurman, Shmuel

    2011-01-01

    The aim of this paper is to present the process for approving and certifying a neonatal intensive care unit to ISO 9001 standards. The process started with the department head's decision to improve services quality before deciding to achieve ISO 9001 certification. Department processes were mapped and quality management mechanisms were developed. Process control and performance measurements were defined and implemented to monitor the daily work. A service satisfaction review was conducted to get feedback from families. In total, 28 processes and related work instructions were defined. Process yields showed service improvements. Family satisfaction improved. The paper is based on preparing only one neonatal intensive care unit to the ISO 9001 standard. The case study should act as an incentive for hospital managers aiming to improve service quality based on the ISO 9001 standard. ISO 9001 is becoming a recommended tool to improve clinical service quality.

  15. Standardization of Image Quality Analysis – ISO 19264

    DEFF Research Database (Denmark)

    Kejser, Ulla Bøgvad; Wüller, Dietmar

    2016-01-01

    There are a variety of image quality analysis tools available for the archiving world, which are based on different test charts and analysis algorithms. ISO has formed a working group in 2012 to harmonize these approaches and create a standard way of analyzing the image quality for archiving...... systems. This has resulted in three documents that have been or are going to be published soon. ISO 19262 defines the terms used in the area of image capture to unify the language. ISO 19263 describes the workflow issues and provides detailed information on how the measurements are done. Last...... but not least ISO 19264 describes the measurements in detail and provides aims and tolerance levels for the different aspects. This paper will present the new ISO 19264 technical specification to analyze image quality based on a single capture of a multi-pattern test chart, and discuss the reasoning behind its...

  16. Usability evaluation of mobile applications using ISO 9241 and ISO 25062 standards.

    Science.gov (United States)

    Moumane, Karima; Idri, Ali; Abran, Alain

    2016-01-01

    This paper presents an empirical study based on a set of measures to evaluate the usability of mobile applications running on different mobile operating systems, including Android, iOS and Symbian. The aim is to evaluate empirically a framework that we have developed on the use of the Software Quality Standard ISO 9126 in mobile environments, especially the usability characteristic. To do that, 32 users had participated in the experiment and we have used ISO 25062 and ISO 9241 standards for objective measures by working with two widely used mobile applications: Google Apps and Google Maps. The QUIS 7.0 questionnaire have been used to collect measures assessing the users' level of satisfaction when using these two mobile applications. By analyzing the results we highlighted a set of mobile usability issues that are related to the hardware as well as to the software and that need to be taken into account by designers and developers in order to improve the usability of mobile applications.

  17. NF-kappaB in Lung Tumorigenesis

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Zhenjian [Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, New York University School of Medicine, 462 First Avenue, NBV 7N24, New York, NY 10016 (United States); Tchou-Wong, Kam-Meng; Rom, William N., E-mail: william.rom@nyumc.org [Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, New York University School of Medicine, 462 First Avenue, NBV 7N24, New York, NY 10016 (United States); Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987 (United States)

    2011-12-14

    The development of lung cancer in humans can be divided into three steps initiation, promotion and progression. This process is driven by alterations in related signal transduction pathways. These pathways signal the aberrant activation of NF-kappaB, a transcription factor that regulates the expression of genes important for lung tumorigenesis. Our current knowledge about the role of the NF-kappaB signaling pathway in the development of lung cancer has been bolstered by animal models demonstrating the connection between K-ras and tobacco induced lung transformation with NF-kappaB. Activation of downstream genes leads to cell proliferation, inhibition of apoptosis, angiogenesis, inflammation, invasion, and metastasis.

  18. AGRO100 inhibits activation of nuclear factor-kappaB (NF-kappaB) by forming a complex with NF-kappaB essential modulator (NEMO) and nucleolin.

    Science.gov (United States)

    Girvan, Allicia C; Teng, Yun; Casson, Lavona K; Thomas, Shelia D; Jüliger, Simone; Ball, Mark W; Klein, Jon B; Pierce, William M; Barve, Shirish S; Bates, Paula J

    2006-07-01

    AGRO100, also known as AS1411, is an experimental anticancer drug that recently entered human clinical trials. It is a member of a novel class of antiproliferative agents known as G-rich oligonucleotides (GRO), which are non-antisense, guanosine-rich phosphodiester oligodeoxynucleotides that form stable G-quadruplex structures. The biological activity of GROs results from their binding to specific cellular proteins as aptamers. One important target protein of GROs has been previously identified as nucleolin, a multifunctional protein expressed at high levels by cancer cells. Here, we report that AGRO100 also associates with nuclear factor-kappaB (NF-kappaB) essential modulator (NEMO), which is a regulatory subunit of the inhibitor of kappaB (IkappaB) kinase (IKK) complex, and also called IKKgamma. In the classic NF-kappaB pathway, the IKK complex is required for phosphorylation of IkappaBalpha and subsequent activation of the transcription factor NF-kappaB. We found that treatment of cancer cells with AGRO100 inhibits IKK activity and reduces phosphorylation of IkappaBalpha in response to tumor necrosis factor-alpha stimulation. Using a reporter gene assay, we showed that AGRO100 blocks both tumor necrosis factor-alpha-induced and constitutive NF-kappaB activity in human cancer cell lines derived from cervical, prostate, breast, and lung carcinomas. In addition, we showed that, in AGRO100-treated cancer cells, NEMO is coprecipitated by nucleolin, indicating that both proteins are present in the same complex. Our studies suggest that abrogation of NF-kappaB activity may contribute to the anticancer effects of AGRO100 and that nucleolin may play a previously unknown role in regulating the NF-kappaB pathway.

  19. Functional conservation of rice OsNF-YB/YC and Arabidopsis AtNF-YB/YC proteins in the regulation of flowering time

    KAUST Repository

    Hwang, Yoon-Hyung

    2016-01-11

    Plant NUCLEAR FACTOR Y (NF-Y) transcription factors play important roles in plant development and abiotic stress. In Arabidopsis thaliana, two NF-YB (AtNF-YB2 and AtNF-YB3) and five NF-YC (AtNF-YC1, AtNF-YC2, AtNF-YC3, AtNF-YC4, and AtNF-YC9) genes regulate photoperiodic flowering by interacting with other AtNF-Y subunit proteins. Three rice NF-YB (OsNF-YB8, OsNF-YB10, and OsNF-YB11) and five rice OsNF-YC (OsNF-YC1, OsNF-YC2, OsNF-YC4, OsNF-YC6, and OsNF-YC7) genes are clustered with two AtNF-YB and five AtNF-YC genes, respectively. To investigate the functional conservation of these NF-YB and NF-YC genes in rice and Arabidopsis, we analyzed the flowering phenotypes of transgenic plants overexpressing the respective OsNF-YB and OsNF-YC genes in Arabidopsis mutants. Overexpression of OsNF-YB8/10/11 and OsNF-YC2 complemented the late flowering phenotype of Arabidopsis nf-yb2 nf-yb3 and nf-yc3 nf-yc4 nf-yc9 mutants, respectively. The rescued phenotype of 35S::OsNF-YC2 nf-yc3 nf-yc4 nf-yc9 plants was attributed to the upregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). In vitro and in planta protein–protein analyses revealed that OsNF-YB8/10/11 and OsNF-YC1/2/4/6/7 interact with AtNF-YC3/4/9 and AtNF-YB2/3, respectively. Our data indicate that some OsNF-YB and OsNF-YC genes are functional equivalents of AtNF-YB2/3 and AtNF-YC3/4/9 genes, respectively, and suggest functional conservation of Arabidopsis and rice NF-Y genes in the control of flowering time.

  20. Litopenaeus vannamei NF-κB is required for WSSV replication.

    Science.gov (United States)

    Qiu, Wei; Zhang, Shuang; Chen, Yong-Gui; Wang, Pei-Hui; Xu, Xiao-Peng; Li, Chao-zheng; Chen, Yi-Hong; Fan, Wen-Zhou; Yan, Hui; Weng, Shao-Ping; FrancisChan, Siuming; He, Jian-Guo

    2014-07-01

    Many viruses can hijack the host cell NF-κB as part of their life cycle, diverting NF-κB immune regulatory functions to favor their replications. There were several reports on the functions of Litopenaeus vannamei NF-κB (LvNF-κB) in White spot syndrome virus (WSSV) replication in vitro. Here, we studied the relationship between LvNF-κB family protein Dorsal (LvDorsal) and Relish (LvRelish) with WSSV replication in vivo. The expressions of LvDorsal and LvRelish were significantly upregulated by WSSV challenge. Virus loads and expression of viral envelope protein VP28 in LvDorsal or LvRelish silencing shrimps were significantly lower than the control shrimps injected with EGFP-dsRNA or PBS after challenge with 1×10(5) copies WSSV/shrimp. In addition to the LvDorsal activation of WSV069 (ie1) and WSV303 promoter that we have reported, LvRelish can also activate WSV069 (ie1) and WSV303 promoter by dual luciferase reporter assays through screening 40 WSSV gene promoters that have putative multiple NF-κB binding sites. The promoter activity of the WSV069 (ie1) by LvDorsal activation was significantly higher than that by LvRelish activation. WSSV replication in LvDorsal, LvRelish or WSV303 silencing shrimps were significantly inhibited. These results indicate that the L. vannamei NF-κB family proteins LvDorsal and LvRelish expressions are significantly activated by WSSV challenge and WSSV replication partially relied on the activations of LvDorsal and LvRelish in vivo. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Prevalence of bortezomib-resistant constitutive NF-kappaB activity in mantle cell lymphoma

    Directory of Open Access Journals (Sweden)

    Kahl Brad S

    2008-05-01

    Full Text Available Abstract Background The proteasome inhibitor bortezomib can inhibit activation of the transcription factor NF-κB, a mechanism implicated in its anti-neoplastic effects observed in mantle cell lymphoma (MCL. However, NF-κB can be activated through many distinct mechanisms, including proteasome independent pathways. While MCL cells have been shown to harbor constitutive NF-κB activity, what fraction of this activity in primary MCL samples is sensitive or resistant to inhibition by bortezomib remains unclear. Results Proteasome activity in the EBV-negative MCL cell lines Jeko-1 and Rec-1 is inhibited by greater than 80% after exposure to 20 nM bortezomib for 4 hours. This treatment decreased NF-κB activity in Jeko-1 cells, but failed to do so in Rec-1 cells when assessed by electrophoretic mobility shift assay (EMSA. Concurrently, Rec-1 cells were more resistant to the cytotoxic effects of bortezomib than Jeko-1 cells. Consistent with a proteasome inhibitor resistant pathway of activation described in mouse B-lymphoma cells (WEHI231 and a breast carcinoma cell line (MDA-MB-468, the bortezomib-resistant NF-κB activity in Rec-1 cells is inhibited by calcium chelators, calmodulin inhibitors, and perillyl alcohol, a monoterpene capable of blocking L-type calcium channels. Importantly, the combination of perillyl alcohol and bortezomib is synergistic in eliciting Rec-1 cell cytotoxicity. The relevance of these results is illuminated by the additional finding that a considerable fraction of primary MCL samples (8 out of 10 displayed bortezomib-resistant constitutive NF-κB activity. Conclusion Our findings show that bortezomib-resistant NF-κB activity is frequently observed in MCL samples and suggest that this activity may be relevant to MCL biology as well as serve as a potential therapeutic target.

  2. Transforming growth factor-beta 1 (TGF-beta1) promotes IL-2 mRNA expression through the up-regulation of NF-kappaB, AP-1 and NF-AT in EL4 cells.

    Science.gov (United States)

    Han, S H; Yea, S S; Jeon, Y J; Yang, K H; Kaminski, N E

    1998-12-01

    Transforming growth factor beta1 (TGF-beta1) has been previously shown to modulate interleukin 2 (IL-2) secretion by activated T-cells. In the present studies, we determined that TGF-beta1 induced IL-2 mRNA expression in the murine T-cell line EL4, in the absence of other stimuli. IL-2 mRNA expression was significantly induced by TGF-beta1 (0.1-1 ng/ml) over a relatively narrow concentration range, which led to the induction of IL-2 secretion. Under identical condition, we examined the effect of TGF-beta1 on the activity of nuclear factor AT (NF-AT), nuclear factor kappaB (NF-kappaB), activator protein-1 (AP-1) and octamer, all of which contribute to the regulation of IL-2 gene expression. Electrophoretic mobility shift assays showed that TGF-beta1 markedly increased NF-AT, NF-kappaB and AP-1 binding to their respective cognate DNA binding sites, whereas octamer binding remained constant, as compared with untreated cells. Employing a reporter gene expression system with p(NF-kappaB)3-CAT, p(NF-AT)3-CAT and p(AP-1)3-CAT, TGF-beta1 treatment of transfected EL4 cells induced a dose-related increase in chloramphenicol acetyltransferase activity that correlated well with the DNA binding profile found in the electrophoretic mobility shift assay studies. These results show that TGF-beta1, in the absence of any additional stimuli, up-regulates the activity of key transcription factors involved in IL-2 gene expression, including NF-AT, NF-kappaB and AP-1, to help promote IL-2 mRNA expression by EL4 cells.

  3. Sertifikatõ ISO - mafioznaja struktura so znakom pljuss / Olesja Bõkova

    Index Scriptorium Estoniae

    Bõkova, Olesja

    2005-01-01

    Esimesed ISO sertifikaadid, mille aluseks on briti kvaliteedi standardite süsteem, anti Euroopa Liidus välja 1987. a. ISO sertifikaadid Eestis. Diagramm: Välja antud ISO 9000 sertifikaadid 1994-2004

  4. ISO Spectroscopy of Proto-Planetary Nebulae

    Science.gov (United States)

    Hrivnak, Bruce J.

    2000-01-01

    The goal of this program was to determine the chemical properties of the dust shells around protoplanetary nebulae (PPNs) through a study of their short-wavelength (6-45 micron) infrared spectra. PPNs are evolved stars in transition from the asymptotic giant branch to the planetary nebula stages. Spectral features in the 10 to 20 gm region indicate the chemical nature (oxygen- or carbon-rich), and the strengths of the features relate to the physical properties of the shells. A few bright carbon-rich PPNs have been observed to show PAH features and an unidentified 21 micron emission feature. We used the Infrared Space Observatory (ISO) to observe a sample of IRAS sources that have the expected properties of PPNs and for which we have accurate positions. Some of these have optical counterparts (proposal SWSPPN01) and some do not (SWSPPN02). We had previously observed these from the ground with near-infrared photometry and, for those with visible counterparts, visible photometry and spectroscopy, which we have combined with these new ISO data in the interpretation of the spectra. We have completed a study of the unidentified emission feature at 21 micron in eight sources. We find the shape of the feature to be the same in all of the sources, with no evidence of any substructure. The ratio of the emission peak to continuum ranges from 0.13 to 1.30. We have completed a study of seven PPNs and two other carbon-rich objects for which we had obtained ISO 2-45 micron observations. The unidentified emission features at 21 and 30 micron were detected in six sources, including four new detections of the 30 micron feature. This previously unresolved 30 micron feature was resolved and found to consist of a broad feature peaking at 27.2 micron (the "30 micron" feature) and a narrower feature peaking at 25.5 micron (the "26 micron" feature). This new 26 micron feature is detected in eight sources and is particularly strong in IRAS Z02229+6208 and 16594-4656. The unidentified

  5. The stability of iso-α-acids and reduced iso-α-acids in stored blood specimens.

    Science.gov (United States)

    Rodda, Luke N; Gerostamoulos, Dimitri; Drummer, Olaf H

    2014-06-01

    The long-term stability of the iso-α-acids, and three structurally similar but chemically altered iso-α-acids (known as 'reduced iso-α-acids' and consisting of the rho-, tetrahydro- and hexahydro-iso-α-acid groups) were investigated in whole blood. Pools of blank blood spiked with the four beer-specific ingredient congener groups at two different concentration levels were stored at 20°C, 4°C and -20°C; and extracted in duplicate in weeks 1, 3, 5 and 8, using a previously published method. A loss of 15% of the initial concentration was considered to indicate possible instability and losses greater than 30% demonstrated significant losses. The individual analytes within the four iso-α-acid groups were also measured to determine which iso-α-acids were subject to greater degradation and were responsible for the overall group instability. All four iso-α-acid groups showed significant losses after 8 weeks of storage under room temperature conditions in particularly the natural iso-α-acid group where major losses were observed (96% and 85% losses for low and high concentrations, respectively). Some degradation in all iso-α-acid groups were seen at 4°C samples predominantly due to the 'n' analogs of the groups showing an increased instability in blood. The -20°C storage conditions resulted in minimal changes in concentrations of all analytes. Higher than frozen storage temperatures can result in substantial changes on the stability of the iso-α-acid type groups in blood. The aim of this study was to highlight the stabilities of the IAA analytes in order to assist in the interpretation of IAA in stored blood specimens. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  6. Evaluation of the role of 8-iso-PGF levels at multiple sites during intracranial hemorrhage in pediatric patients.

    Science.gov (United States)

    Qi, B-X; Yao, H; Shang, L; Sheng, L-P; Wang, X-C; Zhu, L; Zhang, X-X; Wang, J-P; Fang, D-H

    2017-09-01

    The present study was planned to explore the role of 8-isomeric-prostaglandinF2α (8-iso-PGF2α) levels at the multiple sites of cerebrospinal fluid in children with intracranial hemorrhage. 90 children with intracranial hemorrhage were admitted to Surgery Intensive Care Unit (SICU) of our hospital from January to December 2013 and were selected as study subjects. They were divided into group A (n=30), group B (n=30) and group C (n=30). The group A was given conventional treatment, the group B was treated with minimally invasive puncture and the group C was treated with cerebrospinal fluid decompression. After 1 d, 2 d, 3 d, and 7 d of hospitalization, enzyme-linked immunosorbent assay (ELISA) was used to detect the 8-iso-PGF2α levels in peripheral blood of children in all groups. On the day of admission and 10 d after treatment, 3 groups of children were implemented with brain nuclear magnetic resonance spectroscopy for metabolite analyses. On the day of admission there were no significant differences in the 8-iso-PGF2α levels among group A, B and C. Further, after 1 d, 3 d, 7 d of hospital stay, the 8-iso-PGF2α levels in peripheral blood showed a gradual downward trend, and decline range of the group C was greater than that of group A and B (p iso-PGF2α in peripheral blood (r = 0.546, p iso-PGF2α plays an important role in the pathogenesis of intracranial hemorrhage, and could be utilized as a biomarker of oxidative stress in children with intracranial hemorrhage. Further, cerebrospinal fluid decompression is a better method of treatment for intracranial hemorrhage.

  7. NF1 Signal Transduction and Vascular Dysfunction

    Science.gov (United States)

    2014-05-01

    the effects of losing a second allele of NF1 in the vascular endothelium of the adult mouse. This will be the first model of NF1 loss in the... adult endothelium and can serve as a model system for investigation of both cardiovascular effects and the tumor microenvironment. Body: Aim 1...would be to try and determine if there were defects in TGF-b signaling (Smad activation/EndMT) prior to doing a wholesale catalog of all the

  8. ADOPTION OF ISO 9001 QUALITY MANAGEMENT STANDARD IN AFRICA

    Directory of Open Access Journals (Sweden)

    Erlantz Allur

    2014-03-01

    Full Text Available This article analyzes the dissemination of ISO 9001, the main global management standards, within Africa. The work refers to the diffusion of ISO 9001 standard in terms of its certification intensity. In this article, the dissemination of ISO 9001 in Africa has been analyzed. The findings reveal that the diffusion of the standard in this continent is not very relevant, what might be seen as an indicator of the process of Africa's trade marginalization in the age of globalization. The general certification intensity of the continet is of 0.18; in other words, the proportion of the contribution of Africa to the global GDP of the world is more than five times superior to the proportion of ISO 9001 certificates located in Africa. By means of the logistic model the dissemination of ISO 9001 is forecasted, and it has been observed that the diffusion of ISO 9001 in Africa is in an 85% of its saturation point. Taking into account this model, it's expected that the dissemination of ISO 9001 will be growing until 2020.

  9. ISO 26000 – An Integrative Approach of Corporate Social Responsibility

    Directory of Open Access Journals (Sweden)

    Herciu Mihaela

    2016-04-01

    Full Text Available ISO 26000 represents a guidance on corporate social responsibility and it is, at the present time, one of the most important document on CSR in the world. Without being mandatory or regulated, ISO 26000 is focus on seven core subjects that have to be approached synergistic – governance, human right, labor, environment, business practices, consumer, and community – in order to achieve its goal. The present paper intends to present and analyze the seven core subjects of ISO 26000 from tow perspectives: by emphasizing the benefits of implementing the standard into the business strategy and by revealing the correlation between corporate social responsibility and sustainable development.

  10. The historical impact of ISO 9000 on Lebanese firms.

    Science.gov (United States)

    Mezher, Toufic; Ajam, Maher; Shehab, Mohamad

    2004-01-01

    The objective of this paper is to assess the impact of the new ISO 9001:2000 standard on the Quality Management Systems (QMS) of Lebanese firms that were already certified under ISO 9000:1994. To get an accurate feedback of stakeholders in a firm, three different questionnaires were developed and distributed to management, employees, and customers respectively. Empirical results indicate that ISO 9001:2000 has improved the QMS performance of Lebanese firms over that of the 1994 edition. Nevertheless, results showed that the new standard still has some weaknesses when it comes to improving suppliers' relationships and empowering employees.

  11. ISO 9000 series standards: what healthcare facilities need to know.

    Science.gov (United States)

    1995-03-01

    With the recent trend toward implementing or improving quality systems and the increasing frequency with which vendors looking to secure a place in the world market have cited the International Organization for Standardization's (ISO) 9000 Series quality system standards, healthcare facilities have been expressing increased interest in learning about these standards, especially ISO 9001, 9002, and 9003. In this article, we briefly discuss the ISO 9000 Series standards--what they are and what they mean--so that healthcare facilities can properly assess the significance of vendors' statements citing compliance with one or more of these standards.

  12. [ISO 9001:2015 Certification in Quality Management].

    Science.gov (United States)

    Enders, Christian; Lang, Gabriele E; Lang, Gerhard K; Werner, Jens Ulrich

    2017-07-01

    Quality management improves the structures, processes and results of organizations of all kinds. Many practices and clinics have their existing quality management system certified according to ISO 9001, (e.g., to check their own quality management system or to obtain a testimonial against third parties). The latest version ISO 9001:2015 contains some changes, both structurally and in terms of content. These changes can be met with reasonable efforts. An ISO 9001:2015 certification represents a value for your organization, but these advantages are often not directly measurable. Georg Thieme Verlag KG Stuttgart · New York.

  13. First light with ISO and its instruments

    Science.gov (United States)

    1995-12-01

    On 28 November, the first infrared images were taken with the ISOCAM instrument. The target was the beautiful spiral galaxy, M51, also known as the Whirlpool Galaxy. Images were taken in two infrared colours, at wavelengths of 7 and 15 microns, and at two resolutions, namely 3 and 6 arc seconds. Figure 1 shows the 7 micron ISOCAM image with a spatial resolution of 6 arc seconds. Spiral structure in galaxies was first discovered by observations of M51 taken in 1845 by the third Earl of Rosse in Ireland. At a distance of 20 million light years, M51 is not one of our nearest neighbors in space but it is one of the most spectacular, as it presents its spiral structure face-on to us. M51 is smaller and - at a mass of 50000 million suns - less massive than our galaxy. However, due to intense recent star formation, it is much brighter. While very detailed images have been available for some time in the optical and radio wavelength range, infrared data in the ISO range has been very limited. Figure 2 shows the data from the previous infrared satellite - the American/Dutch/British satellite IRAS; where, in a similar wavelength range, there is not even a hint of the spiral structure. For ISOCAM, with its higher spatial resolution and greater sensitivity, not only the spiral structure but also details within are easily visible, even in a raw first light image. Images from ISOCAM data will be compared with maps at other wavelengths to reveal information about the star formation processes in galaxies. First light for the ISOPHOT instrument was obtained on 29 November 1995 from the star Gamma Draconis. A huge signal was measured in a narrow band filter at 3.29 microns (figure 3). The optical axis of the instrument was found by this observation to be as close as 15 arc seconds to the predicted position. The next target was the interacting galaxy, NGC6090, which was clearly detected for the first time at the longest wavelengths, 120 - 240 microns, exclusively available on ISO

  14. Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla

    Directory of Open Access Journals (Sweden)

    Junjun Li

    2014-01-01

    Full Text Available Background Information. NF-κB signaling pathway plays a complicated role in the biological functions of mesenchymal stem cells. However, the effects of NF-κB pathway on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs remain unclear. The present study was designed to evaluate the effects of canonical NF-κB pathway on the osteo/odontogenic capacity of SCAPs in vitro. Results. Western blot results demonstrated that NF-κB pathway in SCAPs was successfully activated by TNF-α or blocked by BMS-345541. NF-κB pathway-activated SCAPs presented a higher proliferation activity compared with control groups, as indicated by dimethyl-thiazol-diphenyl tetrazolium bromide assay (MTT and flow cytometry assay (FCM. Wound scratch assay revealed that NF-κB pathway-activated SCAPs presented an improved migration capacity, enhanced alkaline phosphatase (ALP activity, and upregulated mineralization capacity of SCAPs, as compared with control groups. Meanwhile, the odonto/osteogenic markers (ALP/ALP, RUNX2/RUNX2, OSX/OSX, OCN/OCN, OPN/OPN, BSP/BSP, DSPP/DSP, and DMP-1/DMP-1 in NF-κB pathway-activated SCAPs were also significantly upregulated as compared with control groups at both protein and mRNA levels. However, NF-κB pathway-inhibited SCAPs exhibited a lower proliferation/migration capacity, and decreased odonto/osteogenic ability in comparison with control groups. Conclusion. Our findings suggest that classical NF-κB pathway plays a paramount role in the proliferation and committed differentiation of SCAPs.

  15. ITIL and ISO/IEC 20000 a practical handbook

    CERN Document Server

    Hernandez, Alex

    2012-01-01

    The purpose of this publication is to provide your organization with a pragmatic approach to effectively implementing service management, incorporating practices from the ITIL framework and the ISO/IEC 20000 standard.

  16. ISO and EIGA standards for cryogenic vessels and accessories

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    The EIGA/WG 6’s scope is cryogenic vessels and accessories, including their design, material compatibility, operational requirements and periodical inspection. The specific responsibilities include monitoring international standardization (ISO, CEN) and regulations (UN, TPED, PED...

  17. Applicability of ISO 16697 Data to Spacecraft Fire Fighting Strategies

    Science.gov (United States)

    Hirsch, David B.; Beeson, Harold D.

    2012-01-01

    Presentation Agenda: (1) Selected variables affecting oxygen consumption during spacecraft fires, (2) General overview of ISO 16697, (3) Estimated amounts of material consumed during combustion in typical ISS enclosures, (4) Discussion on potential applications.

  18. ISO 15189 Accreditation: Navigation Between Quality Management and Patient Safety

    National Research Council Canada - National Science Library

    Mario Plebani; Laura Sciacovelli

    2017-01-01

    ... and safety in laboratory medicine. The recent revision of the International Standard, ISO 15189, has further strengthened its value not only for improving the quality system of a clinical laboratory but also for better answering...

  19. NF-κB Participates in the Stem Cell Phenotype of Ovarian Cancer Cells.

    Science.gov (United States)

    Gonzalez-Torres, Carolina; Gaytan-Cervantes, Javier; Vazquez-Santillan, Karla; Mandujano-Tinoco, Edna Ayerim; Ceballos-Cancino, Gisela; Garcia-Venzor, Alfredo; Zampedri, Cecilia; Sanchez-Maldonado, Paulina; Mojica-Espinosa, Raul; Jimenez-Hernandez, Luis Enrique; Maldonado, Vilma

    2017-05-01

    NF-κB is a transcription factor involved in cancer stem cells maintenance of many tumors. Little is known about the specific stem-associated upstream regulators of this pathway in ovarian cancer. The Aim of the study was to analyze the role of the canonical and non-canonical NF-κB pathways in stem cells of ovarian cancer cell lines. Stem cells were isolated using sorting cytometry. Western blot and RT-PCR were used to quantify protein and messenger RNA levels. Loss and gain of function assays were performed using siRNAs and dominant-negative proteins, respectively. NF-κB binding activity was measured with a reporter gene assay. The stem phenotype was estimated with clonogenic assays using soft agar, colony formation, ovospheres formation and in vivo tumorigenicity assays. The CD44+ subpopulation of SKOV3 ovarian cancer cell line presented higher mRNA levels of key stemness genes, an increased tumorigenic capacity and higher expression of the RelA, RelB and IKKα. When the canonical pathway was inhibited by means of a dominant-negative version of IkBα, the stem cell population was reduced, as shown by a reduced CD44+ subpopulation, a decrease in the expression of the stemness genes and a reduction of the stem phenotype. In addition, IKKα, the main upstream non-canonical kinase, was highly expressed in the CSC population. Accordingly, when IKKα was inhibited using shRNAs, the expression of the stemness genes was reduced. This report is the first to show the importance of several elements of both NF-κB pathway in maintaining the ovarian cancer stem cell population. Copyright © 2017 IMSS. Published by Elsevier Inc. All rights reserved.

  20. Geometry-invariant GRIN lens: iso-dispersive contours

    OpenAIRE

    Bahrami, Mehdi; Goncharov, Alexander V.

    2012-01-01

    A dispersive model of a gradient refractive index (GRIN) lens is introduced based on the idea of iso-dispersive contours. These contours have constant Abbe number and their shape is related to iso-indicial contours of the monochromatic geometry-invariant GRIN lens (GIGL) model. The chromatic GIGL model predicts the dispersion throughout the GRIN structure by using the dispersion curves of the surface and the center of the lens. The analytical approach for paraxial ray tracing and the monochro...

  1. ANALIZA I PROCJENA KRETANJA ISO CERTIFIKATA U HRVATSKOJ

    OpenAIRE

    Baldigara, Tea; Brajdić, Igor

    2005-01-01

    Proces certificiranja prema standardima ISO 9000 u posljednjim godinama doživljava izuzetan razvoj, a broj izdanih certifikata eksponencijalno raste. Rad predstavlja analizu stanja i razvoja procesa certificiranja prema standardima ISO 9000 u Svijetu i Europi s posebnim naglaskom na Hrvatsku. Usporedbe i predviđanja temelje se na logističkom modelu prvobitno upotrebljavanom u analizama razvoja bioloških sustava.

  2. Penerapan Total Quality Management dan ISO 9000 dalam Pendidikan Teknik

    Directory of Open Access Journals (Sweden)

    Ahmad Sonhadji K. Hasan

    2016-02-01

    Full Text Available An organization is able to improve and maintain its quality by building a quality improvement system, and determining the quality standards. For satisfying industry requirements in providing human resources, technical education must continuously improve its quality. The need can be effectively achieved by appliying TQM and ISO 9000. TQM is a quality improvement system for meeting customer requirements, and ISO 9000 is an international certification for standards of design, production, instalation, and servicing

  3. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-κB and NF-κB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis.

    Science.gov (United States)

    Kim, Ji H; Gupta, Subash C; Park, Byoungduck; Yadav, Vivek R; Aggarwal, Bharat B

    2012-03-01

    The incidence of cancer is significantly lower in regions where turmeric is heavily consumed. Whether lower cancer incidence is due to turmeric was investigated by examining its effects on tumor cell proliferation, on pro-inflammatory transcription factors NF-κB and STAT3, and on associated gene products. Cell proliferation and cell cytotoxicity were measured by the MTT method, NF-κB activity by EMSA, protein expression by Western blot analysis, ROS generation by FACS analysis, and osteoclastogenesis by TRAP assay. Turmeric inhibited NF-κB activation and down-regulated NF-κB-regulated gene products linked to survival (Bcl-2, cFLIP, XIAP, and cIAP1), proliferation (cyclin D1 and c-Myc), and metastasis (CXCR4) of cancer cells. The spice suppressed the activation of STAT3, and induced the death receptors (DR)4 and DR5. Turmeric enhanced the production of ROS, and suppressed the growth of tumor cell lines. Furthermore, turmeric sensitized the tumor cells to chemotherapeutic agents capecitabine and taxol. Turmeric was found to be more potent than pure curcumin for cell growth inhibition. Turmeric also inhibited NF-κB activation induced by RANKL that correlated with the suppression of osteoclastogenesis. Our results indicate that turmeric can effectively block the proliferation of tumor cells through the suppression of NF-κB and STAT3 pathways. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Inter ISO Market Coordination by Calculating Border Locational Marginal Prices

    Directory of Open Access Journals (Sweden)

    BABIC, A. B.

    2013-05-01

    Full Text Available In this paper the methodology for solving Locational Marginal Price (LMP differences (inconsistency of LMPs that arise at the boundary buses between separate power markets is proposed. The algorithm developed enables us to obtain consistent LMP values at the boundary buses between interconnected ISOs. A Primal-Dual Interior Point based optimal power flow (OPF is applied, with complete set of power system physical limit constraints, to solve a regional spot market. The OPF is implemented such that producer and consumer behaviors are modeled simultaneously, while the welfare is maximized. In this paper a generalized methodology for multiple ISOs case is proposed and later it is practically applied on two interconnected independent entities. The algorithm for approximation of cost coefficients of generators and dispatchable loads for neighboring ISOs is proposed. The developed algorithm enables participating ISOs to obtain LMPs at the boundary buses with other interconnected ISOs. By controlling interchange of electric power at the scheduled level, regional spot markets are resolved eliminating possible exercise of market power by individual interconnected ISOs. Results of proposed methodology are tested on the IEEE 118-bus power system.

  5. Cervical neurofibromas in children with NF-1

    Energy Technology Data Exchange (ETDEWEB)

    Chung, C.J.; Mukherji, S.K.; Fordham, L.A. [University of North Carolina, Chapel Hill (United States). Dept. of Radiology; Armfield, K.B.; Krause, W.L. [Scottish Rite Children`s Medical Center, AtlantA. GA (United States). Dept. of Genetics

    1999-05-01

    Background. Children with neurofibromatosis type 1 (NF1) are at increased risk of developing plexiform neurofibroma throughout the body, including the cervical soft tissues. However, the incidence of cervical soft tissue tumors and the value of screening MR for children with NF1 are not known. Purpose. The purposes of this study were to determine the incidence and clinical significance of cervical tumors seen on MR imaging in children with NF1. Materials and methods. A retrospective review of the brain and orbit MR with cervical images obtained on 95 children who meet the NIH consensus criteria for NF1 and who are followed at our neurofibromatosis clinic was carried out. Results. Cervical tumors were found on MR imaging in 21 of 95 (22 %) children. Of 21 children with cervical tumors, 14 children were determined to be surgical candidates. In nine children, MR imaging altered the clinical management by demonstrating tumors for which surgery was indicated, but the tumors were not suspected prior to MR imaging. Conclusion. Cervical tumors are commonly seen in children with NF1. MR imaging may demonstrate a significant number of tumors that require surgery, but were not suspected prior to MR imaging. (orig.) With 3 figs., 1 tab., 17 refs.

  6. Asian sand dust causes subacute peripheral immune modification with NF-κB activation.

    Science.gov (United States)

    Song, Yuan; Ichinose, Takamichi; Morita, Kentaro; Nakanishi, Tsukasa; Kanazawa, Tamotsu; Yoshida, Yasuhiro

    2015-05-01

    Asian sand dust (ASD), a type of particulate matter found in Asia, migrates to East Asia. The increased airborne spread of ASD has led to concerns regarding possible adverse health effects. Our group previously reported that ASD induces lung inflammation in mice, but it is still unclear whether ASD affects lymphoid organs. In this study, we investigated the effect of ASD on splenocytes in a mouse model of ASD exposure. ICR mice were intratracheally administered a single dose of normal saline (control) or ASD and were subsequently sacrificed 1 or 3 days later. TNF-α production in bronchoalveolar lavage fluids was higher at day 1, but not at day 3, after ASD administration. The enzyme-linked immunosorbent assay results showed that ASD administration increased mitogen-induced IL-2, TNF-α, and IL-6 production in splenocytes. Additionally, cell viability assay showed enhanced splenocyte proliferation at day 3, but not at day 1, after ASD administration. The electrophoretic mobility shift assay results demonstrated that nuclear factor κB (NF-κB) was activated in splenocytes on day 3, but not on day 1. In particular, NF-κB activation was detected in CD4(+) and CD11b(+) cells on day 3. These results suggest that ASD induces subacute inflammatory responses with NF-κB activation in the spleen, in contrast to acute inflammation in the lungs. © 2013 Wiley Periodicals, Inc.

  7. Bmi-1 promotes the aggressiveness of glioma via activating the NF-kappaB/MMP-9 signaling pathway

    Directory of Open Access Journals (Sweden)

    Jiang Lili

    2012-09-01

    Full Text Available Abstract Background The prognosis of human glioma is poor, and the highly invasive nature of the disease represents a major impediment to current therapeutic modalities. The oncoprotein B-cell-specific Moloney murine leukemia virus integration site 1 protein (Bmi-1 has been linked to the development and progression of glioma; however, the biological role of Bmi-1 in the invasion of glioma remains unclear. Methods A172 and LN229 glioma cells were engineered to overexpress Bmi-1 via stable transfection or to be silenced for Bmi-1 expression using RNA interfering method. Migration and invasiveness of the engineered cells were assessed using wound healing assay, Transwell migration assay, Transwell matrix penetration assay and 3-D spheroid invasion assay. MMP-9 expression and activity were measured using real-time PCR, ELISA and the gelatin zymography methods. Expression of NF-kappaB target genes was quantified using real-time PCR. NF-kappaB transcriptional activity was assessed using an NF-kappaB luciferase reporter system. Expression of Bmi-1 and MMP-9 in clinical specimens was analyzed using immunohistochemical assay. Results Ectopic overexpression of Bmi-1 dramatically increased, whereas knockdown of endogenous Bmi-1 reduced, the invasiveness and migration of glioma cells. NF-kappaB transcriptional activity and MMP-9 expression and activity were significantly increased in Bmi-1-overexpressing but reduced in Bmi-1-silenced cells. The reporter luciferase activity driven by MMP-9 promoter in Bmi-1-overexpressing cells was dependent on the presence of a functional NF-kappaB binding site, and blockade of NF-kappaB signaling inhibited the upregulation of MMP-9 in Bmi-1 overexpressing cells. Furthermore, expression of Bmi-1 correlated with NF-kappaB nuclear translocation as well as MMP-9 expression in clinical glioma samples. Conclusions Bmi-1 may play an important role in the development of aggressive phenotype of glioma via activating the NF

  8. Bmi-1 promotes the aggressiveness of glioma via activating the NF-kappaB/MMP-9 signaling pathway

    Science.gov (United States)

    2012-01-01

    Background The prognosis of human glioma is poor, and the highly invasive nature of the disease represents a major impediment to current therapeutic modalities. The oncoprotein B-cell-specific Moloney murine leukemia virus integration site 1 protein (Bmi-1) has been linked to the development and progression of glioma; however, the biological role of Bmi-1 in the invasion of glioma remains unclear. Methods A172 and LN229 glioma cells were engineered to overexpress Bmi-1 via stable transfection or to be silenced for Bmi-1 expression using RNA interfering method. Migration and invasiveness of the engineered cells were assessed using wound healing assay, Transwell migration assay, Transwell matrix penetration assay and 3-D spheroid invasion assay. MMP-9 expression and activity were measured using real-time PCR, ELISA and the gelatin zymography methods. Expression of NF-kappaB target genes was quantified using real-time PCR. NF-kappaB transcriptional activity was assessed using an NF-kappaB luciferase reporter system. Expression of Bmi-1 and MMP-9 in clinical specimens was analyzed using immunohistochemical assay. Results Ectopic overexpression of Bmi-1 dramatically increased, whereas knockdown of endogenous Bmi-1 reduced, the invasiveness and migration of glioma cells. NF-kappaB transcriptional activity and MMP-9 expression and activity were significantly increased in Bmi-1-overexpressing but reduced in Bmi-1-silenced cells. The reporter luciferase activity driven by MMP-9 promoter in Bmi-1-overexpressing cells was dependent on the presence of a functional NF-kappaB binding site, and blockade of NF-kappaB signaling inhibited the upregulation of MMP-9 in Bmi-1 overexpressing cells. Furthermore, expression of Bmi-1 correlated with NF-kappaB nuclear translocation as well as MMP-9 expression in clinical glioma samples. Conclusions Bmi-1 may play an important role in the development of aggressive phenotype of glioma via activating the NF-kappaB/MMP-9 pathway and therefore

  9. NF-kappa B modulation is involved in celastrol induced human multiple myeloma cell apoptosis.

    Directory of Open Access Journals (Sweden)

    Haiwen Ni

    Full Text Available Celastrol is an active compound extracted from the root bark of the traditional Chinese medicine Tripterygium wilfordii Hook F. To investigate the effect of celastrol on human multiple myeloma cell cycle arrest and apoptosis and explore its molecular mechanism of action. The activity of celastrol on LP-1 cell proliferation was detected by WST-8 assay. The celastrol-induced cell cycle arrest was analyzed by flow cytometry after propidium iodide staining. Nuclear translocation of the nuclear factor kappa B (NF-κB was observed by fluorescence microscope. Celastrol inhibited cell proliferation of LP-1 myeloma cell in a dose-dependent manner with IC50 values of 0.8817 µM, which was mediated through G1 cell cycle arrest and p27 induction. Celastrol induced apoptosis in LP-1 and RPMI 8226 myeloma cells in a time and dose dependent manner, and it involved Caspase-3 activation and NF-κB pathway. Celastrol down-modulated antiapoptotic proteins including Bcl-2 and survivin expression. The expression of NF-κB and IKKa were decreased after celastrol treatment. Celastrol effectively blocked the nuclear translocation of the p65 subunit and induced human multiple myeloma cell cycle arrest and apoptosis by p27 upregulation and NF-kB modulation. It has been demonstrated that the effect of celastrol on NF-kB was HO-1-independent by using zinc protoporphyrin-9 (ZnPPIX, a selective heme oxygenase inhibitor. From the results, it could be inferred that celastrol may be used as a NF-kB inhibitor to inhibit myeloma cell proliferation.

  10. IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay.

    Science.gov (United States)

    Kadoya, Masato; Kaida, Kenichi; Koike, Haruki; Takazaki, Hiroshi; Ogata, Hidenori; Moriguchi, Kota; Shimizu, Jun; Nagata, Eiichiro; Takizawa, Shunya; Chiba, Atsuro; Yamasaki, Ryo; Kira, Jun-Ichi; Sobue, Gen; Ikewaki, Katsunori

    2016-12-15

    We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negative sera. Fifteen CIDP patients (8%) were IgG4 anti-human NF155 antibody-positive, which were confirmed by western blot, cell-based assay and immunohistochemical study. None of disease controls or healthy subjects had positive results. Clinical presentation of IgG4 anti-NF155 antibody-positive patients was consistent with those in previous reports. This ELISA combined with determination of the IgG4 subclass is useful in screening for anti-NF155 antibodies. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Bone Morphogenetic Protein 2 Mediates Dentin Sialophosphoprotein Expression and Odontoblast Differentiation via NF-Y Signaling*S⃞

    Science.gov (United States)

    Chen, Shuo; Gluhak-Heinrich, Jelica; Martinez, Marcos; Li, Tong; Wu, Yimin; Chuang, Hui-Hsiu; Chen, Lei; Dong, Juan; Gay, Isabel; MacDougall, Mary

    2008-01-01

    Dentin sialophosphoprotein (DSPP), an important odontoblast differentiation marker, is necessary for tooth development and mineralization. Bone morphogenetic protein 2 (BMP2) plays a vital role in odontoblast function via diverse signal transduction systems. We hypothesize that BMP2 regulates DSPP gene transcription and thus odontoblast differentiation. Here we report that expression of BMP2 and DSPP is detected during mouse odontogenesis by in situ hybridization assay, and BMP2 up-regulates DSPP mRNA and protein expression as well as DSPP-luciferase promoter activity in mouse preodontoblasts. By sequentially deleting fragments of the mouse DSPP promoter, we show that a BMP2-response element is located between nucleotides –97 and –72. By using antibody and oligonucleotide competition assays in electrophoretic mobility shift analysis and chromatin immunoprecipitation experiments, we show that the heterotrimeric transcription factor Y (NF-Y) complex physically interacts with the inverted CCAAT box within the BMP2-response element. BMP2 induces NF-Y accumulation into the nucleus increasing its recruitment to the mouse DSPP promoter in vivo. Furthermore, forced overexpression of NF-Y enhances promoter activity and increases endogenous DSPP protein levels. In contrast, mutations in the NF-Y-binding motif reduce BMP2-induced DSPP transcription. Moreover, inhibiting BMP2 signaling by Noggin, a BMP2 antagonist, results in significant inhibition of DSPP gene expression in preodontoblasts. Taken together, these results indicate that BMP2 mediates DSPP gene expression and odontoblast differentiation via NF-Y signaling during tooth development. PMID:18424784

  12. Cumprimento das normas ISO 14001 e ISO 22000 por serviços de alimentação

    OpenAIRE

    Laura Lyra Santos; Rita de Cássia Coelho de Almeida Akutsu; Raquel Braz Assunção Botelho; Renata Puppin Zandonadi

    2012-01-01

    OBJECTIVE: The objective of this study was to assess food service environmental and food safety management systems according to two checklists based on ABNT ISO 22000 and 14001. METHODS: This exploratory and descriptive study investigated a-la-carte food services of the Federal District, Brazil. Two checklists were developed to investigate ISO 14001 and 22000 compliance. A total of 37 food services were selected from the list of the Brazilian Association of Bars and Restaurants by simple rand...

  13. Pentacyclic Triterpenoids Inhibit IKKβ Mediated Activation of NF-κB Pathway: In Silico and In Vitro Evidences.

    Directory of Open Access Journals (Sweden)

    Kalpesh R Patil

    Full Text Available Pentacyclic Triterpenoids (PTs and their analogues as well as derivatives are emerging as important drug leads for various diseases. They act through a variety of mechanisms and a majority of them inhibit the nuclear factor kappa-beta (NF-κB signaling pathway. In this study, we examined the effects of the naturally occurring PTs on IκB kinase-β (IKKβ, which has great scientific relevance in the NF-κB signaling pathway. On virtual screening, 109 PTs were screened through the PASS (prediction of activity spectra of substances software for prediction of NF-κB inhibitory activity followed by docking on the NEMO/IKKβ association complex (PDB: 3BRV and testing for compliance with the softened Lipinski's Rule of Five using Schrodinger (LLC, New York, USA. Out of the projected 45 druggable PTs, Corosolic Acid (CA, Asiatic Acid (AA and Ursolic Acid (UA were assayed for IKKβ kinase activity in the cell free medium. The UA exhibited a potent IKKβ inhibitory effect on the hotspot kinase assay with IC50 of 69 μM. Whereas, CA at 50 μM concentration markedly reduced the NF-κB luciferase activity and phospho-IKKβ protein expressions. The PTs tested, attenuated the expression of the NF-κB cascade proteins in the LPS-stimulated RAW 264.7 cells, prevented the phosphorylation of the IKKα/β and blocked the activation of the Interferon-gamma (IFN-γ. The results suggest that the IKKβ inhibition is the major mechanism of the PTs-induced NF-κB inhibition. PASS predictions along with in-silico docking against the NEMO/IKKβ can be successfully applied in the selection of the prospective NF-κB inhibitory downregulators of IKKβ phosphorylation.

  14. Orf virus 002 protein targets ovine protein S100A4 and inhibits NF-kappa B signaling

    Directory of Open Access Journals (Sweden)

    Daxiang Chen

    2016-09-01

    Full Text Available Orf virus (ORFV, a member of Parapoxvirus, has evolved various strategies to modulate the immune responses of host cells. The ORFV-encoded protein ORFV002, a regulator factor, has been found to inhibit the acetylation of NF-κB-p65 by blocking phosphorylation of NF-kB-p65 at Ser276 and also to disrupt the binding of NF-kB-p65 and p300. To explore the mechanism by which ORFV002 regulates NF-κB signaling, the understanding of ORFV002 potential binding partners in host cells is critical. In this study, ovine S100 calcium binding protein A4 (S100A4, prolylendopeptidase-like (PREPL and NADH dehydrogenase (ubiquinone 1 alpha subcomplex 8 (NDUFA8 were found to interact with ORFV002 based on the yeast two-hybrid (Y2H assay using a cDNA library derived from primary ovine fetal turbinate cells (OFTu. GST pull-down and bidirectional co-immunoprecipitation assay results demonstrate that ORFV002 interacts with S100A4 directly. Following the pEGFP-ORFV002 (p002GFP transfection, we found that cytoplasmic S100A4 translocates into the nucleus and co-localizes with ORFV002. Furthermore, the inhibitory effect of ORFV002 on NF-κB signaling was significantly restored by S100A4 knock-down phenotype, suggesting ovine S100A4 participating in the ORFV002-mediated NF-κB signaling. These data demonstrate that ORFV002 inhibits the NF-κB activation through its interaction with S100A4 along with its nucleus translocation.

  15. 77 FR 11532 - Notice of Attendance at ISO New England and NEPOOL Meetings

    Science.gov (United States)

    2012-02-27

    ... Federal Energy Regulatory Commission Notice of Attendance at ISO New England and NEPOOL Meetings The... Commission staff may attend upcoming ISO New England Inc. (ISO-NE) and New England Power Pool (NEPOOL... Commission staff may monitor the various meetings posted on the ISO-NE Web site. NEPOOL Participants...

  16. From ISO 9001:2008 to ISO 9001:2015: Significant changes and their impacts to aspiring organizations

    Science.gov (United States)

    Sari, Y.; Wibisono, E.; Wahyudi, R. D.; Lio, Y.

    2017-11-01

    ISO 9001:2015 is the latest version of ISO Quality Management System standard that has been updated recently from ISO 9001:2008. It is necessary for all organizations that have implemented and been certified with ISO 9001:2008 to prepare the transition and upgrade their Quality Management System because the certification will expire by September 2018. This paper attempts to provide knowledge on the significant changes from ISO 9001:2008 to ISO 9001:2015, what new requirements are added, and how they would impact the organizations. An exploratory and applied research was chosen as the research approach and aimed to explore what transition designs are needed to anticipate the changes as well as their impacts. The research applied a methodology of Plan-Do-Check-Action (PDCA) cycle into four organizations and their results were compared and discussed to explain the transition designs. Some qualitative methods such as observation and interview were used to collect the data. By addressing the new requirements, three transition designs that should be prepared are: (i) identifying needs from interested parties, (ii) analyzing internal and external factors of the organizations to formulate relevant strategies and quality objectives, and (iii) registering risks associated to business processes as well as organizational strategies.

  17. Investigating NF-κB signaling in lung fibroblasts in 2D and 3D culture systems.

    Science.gov (United States)

    Htwe, Su Su; Harrington, Helen; Knox, Alan; Rose, Felicity; Aylott, Jonathan; Haycock, John W; Ghaemmaghami, Amir M

    2015-12-01

    Inflammatory respiratory diseases are amongst major global health challenges. Lung fibroblasts have been shown to play a key role in lung inflammatory responses. However, their exact role in initiation and maintenance of lung diseases has remained elusive partly due to the limited availability of physiologically relevant in vitro models. Therefore, developing new tools that enable investigating the molecular pathways (e.g. nuclear factor-kappa B (NF-κB) activation) that underpin inflammatory responses in fibroblasts could be a valuable resource for scientists working in this area of research. In order to investigate NF-κB activation in response to pro-inflammatory stimuli in real-time, we first developed two detection systems based on nuclear localization of NF-κB by immunostaining and luciferase reporter assay system. Furthermore using electrospun porous scaffolds, with similar geometry to human lung extracellular matrix, we developed 3D cultures of lung fibroblasts allowing comparing NF-κB activation in response to pro-inflammatory stimuli (i.e. TNF-α) in 2D and 3D. Our data clearly show that the magnitude of NF-κB activation in 2D cultures is substantially higher than 3D cultures. However, unlike 2D cultures, cells in the 3D model remained responsive to TNF-α at higher concentrations. The more subdued and wider dynamic range of NF-κB responses in 3D culture system was associated with a different expression pattern for TNF receptor I in 3D versus 2D cultures collectively reflecting a more in vivo like TNF receptor I expression and NF-κB activation pattern in the 3D system. Our data suggest that lung fibroblasts are actively involved in the pathogenesis of lung inflammation by activation of NF-κB signaling pathway. The 3D culture detection system provides a sensitive and biologically relevant tool for investigating different pro-inflammatory events involving lung fibroblasts.

  18. Maternal Wnt/β-catenin signaling coactivates transcription through NF-κB binding sites during Xenopus axis formation.

    Directory of Open Access Journals (Sweden)

    Neil J Armstrong

    Full Text Available Maternal Wnt/β-Catenin signaling establishes a program of dorsal-specific gene expression required for axial patterning in Xenopus. We previously reported that a subset of dorsally expressed genes depends not only on Wnt/β-Catenin stimulation, but also on a MyD88-dependent Toll-like receptor/IL1-receptor (TLR/IL1-R signaling pathway. Here we show that these two signal transduction cascades converge in the nucleus to coactivate gene transcription in blastulae through a direct interaction between β-Catenin and NF-κB proteins. A transdominant inhibitor of NF-κB, ΔNIκBα, phenocopies loss of MyD88 protein function, implicating Rel/NF-κB proteins as selective activators of dorsal-specific gene expression. Sensitive axis formation assays in the embryo demonstrate that dorsalization by Wnt/β-Catenin requires NF-κB protein activity, and vice versa. Xenopus nodal-related 3 (Xnr3 is one of the genes with dual β-Catenin/NF-κB input, and a proximal NF-κB consensus site contributes to the regional activity of its promoter. We demonstrate in vitro binding of Xenopus β-Catenin to several XRel proteins. This interaction is observed in vivo upon Wnt-stimulation. Finally, we show that a synthetic luciferase reporter gene responds to both endogenous and exogenous β-Catenin levels in an NF-κB motif dependent manner. These results suggest that β-Catenin acts as a transcriptional co-activator of NF-κB-dependent transcription in frog primary embryonic cells.

  19. Maternal Wnt/β-Catenin Signaling Coactivates Transcription through NF-κB Binding Sites during Xenopus Axis Formation

    Science.gov (United States)

    Armstrong, Neil J.; Fagotto, François; Prothmann, Christian; Rupp, Ralph A. W.

    2012-01-01

    Maternal Wnt/β-Catenin signaling establishes a program of dorsal-specific gene expression required for axial patterning in Xenopus. We previously reported that a subset of dorsally expressed genes depends not only on Wnt/β-Catenin stimulation, but also on a MyD88-dependent Toll-like receptor/IL1-receptor (TLR/IL1-R) signaling pathway. Here we show that these two signal transduction cascades converge in the nucleus to coactivate gene transcription in blastulae through a direct interaction between β-Catenin and NF-κB proteins. A transdominant inhibitor of NF-κB, ΔNIκBα, phenocopies loss of MyD88 protein function, implicating Rel/NF-κB proteins as selective activators of dorsal-specific gene expression. Sensitive axis formation assays in the embryo demonstrate that dorsalization by Wnt/β-Catenin requires NF-κB protein activity, and vice versa. Xenopus nodal-related 3 (Xnr3) is one of the genes with dual β-Catenin/NF-κB input, and a proximal NF-κB consensus site contributes to the regional activity of its promoter. We demonstrate in vitro binding of Xenopus β-Catenin to several XRel proteins. This interaction is observed in vivo upon Wnt-stimulation. Finally, we show that a synthetic luciferase reporter gene responds to both endogenous and exogenous β-Catenin levels in an NF-κB motif dependent manner. These results suggest that β-Catenin acts as a transcriptional co-activator of NF-κB-dependent transcription in frog primary embryonic cells. PMID:22590521

  20. Persistent activation of NF-kappaB related to IkappaB's degradation profiles during early chemical hepatocarcinogenesis

    Directory of Open Access Journals (Sweden)

    García-Román Rebeca

    2007-04-01

    Full Text Available Abstract Background To define the NF-kappaB activation in early stages of hepatocarcinogenesis and its IkappaB's degradation profiles in comparison to sole liver regeneration. Methods Western-blot and EMSA analyses were performed for the NF-kappaB activation. The transcriptional activity of NF-kappaB was determined by RT-PCR of the IkappaB-α mRNA. The IkappaB's degradation proteins were determined by Western-blot assay. Results We demonstrated the persistent activation of NF-kappaB during early stages of hepatocarcinogenesis, which reached maximal level 30 min after partial hepatectomy. The DNA binding and transcriptional activity of NF-kappaB, were sustained during early steps of hepatocarcinogenesis in comparison to only partial hepatectomy, which displayed a transitory NF-kappaB activation. In early stages of hepatocarconogenesis, the IkappaB-α degradation turned out to be acute and transitory, but the low levels of IkappaB-β persisted even 15 days after partial hepatectomy. Interestingly, IkappaB-β degradation is not induced after sole partial hepatectomy. Conclusion We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-β and IkappaB-α degradation, mainly IkappaB-β degradation, which contributes to gene transcription related to proliferation required for neoplasic progression.

  1. Decreased osteogenesis in mesenchymal stem cells derived from the aged mouse is associated with enhanced NF-κB activity.

    Science.gov (United States)

    Lin, Tzu-Hua; Gibon, Emmanuel; Loi, Florence; Pajarinen, Jukka; Córdova, Luis A; Nabeshima, Akira; Lu, Laura; Yao, Zhenyu; Goodman, Stuart B

    2017-02-01

    Aging is associated with significant bone loss and delayed fracture healing. NF-κB activation is highly correlated with inflammatory-associated bone diseases including infection, wear particle exposure, and chronic inflammation during natural aging processes. The critical roles of NF-κB in both the pro-inflammatory response and osteoclast-mediated bone resorption have been well defined. However, the biological effects of NF-κB activation in mesenchymal stem cell (MSC)-mediated bone formation remain largely unknown. In the current study, bone marrow-MSCs were isolated from young (8 weeks old) and aged (72 weeks old) mice. NF-κB activity in MSCs at basal levels and under different biological conditions were determined by our recently established lentiviral vector-based luciferase reporter assay. We found that NF-κB activity was increased in aged MSCs at basal levels or when exposed to low dose (10 or 100 ng/ml) lipopolysaccharide (LPS); this effect was not seen when the cells were exposed to higher dose (1 μg/ml) LPS. During osteogenesis, NF-κB activity was increased in aged MSCs at weeks 1 and 2, but showed no significant difference at week 3. Both Smurf2 and TAZ, the NF-κB target genes that regulate osteogenic differentiation, were increased in aged MSCs. In addition, the expression of RANKL was dramatically increased, and OPG was decreased in aged MSCs. Our findings suggest that targeting NF-κB activity in MSCs has the potential to modulate aging-associated bone loss, or enhance bone-healing in aged patients. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:281-288, 2017. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

  2. Regulation of NF-{kappa}B activity in astrocytes: effects of flavonoids at dietary-relevant concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Spilsbury, Alison [Reading School of Pharmacy, University of Reading, Reading RG6 6UB (United Kingdom); Vauzour, David; Spencer, Jeremy P.E. [Molecular Nutrition Group, Centre for Integrative Neuroscience and Neurodynamics, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP (United Kingdom); Rattray, Marcus, E-mail: m.a.n.rattray@reading.ac.uk [Reading School of Pharmacy, University of Reading, Reading RG6 6UB (United Kingdom)

    2012-02-17

    Highlights: Black-Right-Pointing-Pointer We tested the hypothesis that low concentrations of flavonoids inhibit NF-{kappa}B in astrocytes. Black-Right-Pointing-Pointer Primary cultured astrocytes possess a functional {kappa}B-system, measured using luciferase assays. Black-Right-Pointing-Pointer Seven flavonoids (100 nM-1 {mu}M) failed to reduce NF-{kappa}B activity in astrocytes. Black-Right-Pointing-Pointer Four flavonoids (100 nM-1 {mu}M) failed to reduce TNFa-stimulated NF-{kappa}B activity in astrocytes. Black-Right-Pointing-Pointer (-)-Epicatechin did not regulate nuclear translocation of the NF-{kappa}B subunit, p65. -- Abstract: Neuroinflammation plays an important role in the progression of neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Sustained activation of nuclear transcription factor {kappa}B (NF-{kappa}B) is thought to play an important role in the pathogenesis of neurodegenerative disorders. Flavonoids have been shown to possess antioxidant and anti-inflammatory properties and we investigated whether flavonoids, at submicromolar concentrations relevant to their bioavailability from the diet, were able to modulate NF-{kappa}B signalling in astrocytes. Using luciferase reporter assays, we found that tumour necrosis factor (TNF{alpha}, 150 ng/ml) increased NF-{kappa}B-mediated transcription in primary cultures of mouse cortical astrocytes, which was abolished on co-transfection of a dominant-negative I{kappa}B{alpha} construct. In addition, TNF{alpha} increased nuclear localisation of p65 as shown by immunocytochemistry. To investigate potential flavonoid modulation of NF-{kappa}B activity, astrocytes were treated with flavonoids from different classes; flavan-3-ols ((-)-epicatechin and (+)-catechin), flavones (luteolin and chrysin), a flavonol (kaempferol) or the flavanones (naringenin and hesperetin) at dietary-relevant concentrations (0.1-1 {mu}M) for 18 h. None of the flavonoids modulated constitutive or

  3. Helicobacter pylori-induced cell death is counteracted by NF-κB-mediated transcription of DARPP-32.

    Science.gov (United States)

    Zhu, Shoumin; Soutto, Mohammed; Chen, Zheng; Peng, DunFa; Romero-Gallo, Judith; Krishna, Uma S; Belkhiri, Abbes; Washington, M Kay; Peek, Richard; El-Rifai, Wael

    2017-05-01

    DARPP-32 is a frequently amplified and overexpressed gene that promotes several oncogenic functions in gastric cancer. Herein, we investigated the relationship between Helicobacter pylori infection, proinflammatory NF-κB activation and regulation of DARPP-32. The study used in vivo and in vitro experiments. Luciferase reporter, quantitative real-time PCR, immunoblot, chromatin immunoprecipitation (ChIP), cell viability, H. pylori infection, tissue microarrays and immunohistochemical assays were used. Our results indicated that H. pylori infection increased the DARPP-32 mRNA and protein levels in gastric cancer cell lines and gastric mucosa of mice. H. pylori infection increased the activity of NF-κB reporter and p-NF-κB (S536) protein level in vitro and in vivo. To investigate the transcriptional regulation of DARPP-32, we cloned a 3019 bp of the DARPP-32 promoter into the luciferase reporter (pGL3-Luc). Both H. pylori infection and tumour necrosis factor-α treatment induced DARPP-32 reporter activity (p32 promoter and ChIP assay, we demonstrated that the sequence -996 to -1008 bp containing putative NF-κB-binding sites is the most active region. The induction of DARPP-32 expression by H. pylori infection counteracted H. pylori-induced cell death through activation of serine/threonine-specific protein kinase (AKT), as determined by ATP-Glo and clonogenic survival assays. Immunohistochemistry analysis demonstrated a significant positive correlation between NF-κB and DARPP-32 expression levels in gastric cancer tissues (r(2)=0.43, p32 overexpression and its prosurvival oncogenic functions, the induction of DARPP-32 expression following H. pylori infection and activation of NF-κB provides a link between infection, inflammation and gastric tumourigenesis. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  4. Livscyklusvurderinger - en kommenteret oversættelse af ISO 14040 til 14043

    DEFF Research Database (Denmark)

    Jerland, J.; Christiansen, K.; Weidema, B.

    . Standarderne er DS/EN ISO 14040 Livscyklusvurdering. Principper og opbygning DS/EN ISO 14041 Livscyklusvurdering. Formål og afgrænsning DS/EN ISO 14042 Livscyklusvurdering. Vurdering af miljøpåvirkninger DS/EN ISO 14043 Livscyklusvurdering. Fortolkning af resultater Målet med publikationen er at give brugerne...... af livscyklusvurderinger et samlet værktøj som udgangspunkt for arbejdet med LCA efter ISO-standarderne i 14040 serien....

  5. A Functional Genomic Analysis of NF1-Associated Learning Disabilities

    National Research Council Canada - National Science Library

    Tang, Shao-Jun

    2008-01-01

    Learning disabilities severely deteriorate the life of many NF1 patients. However, the pathogenic process for NF1-associated learning disabilities has not been fully understood and an effective therapy is not available...

  6. A Functional Genomic Analysis of NF1-Associated Learning Disabilities

    National Research Council Canada - National Science Library

    Tang, Shao-Jun

    2007-01-01

    Learning disabilities severely deteriorate the life of many NF1 patients. However, the pathogenic process for NF1-associated learning disabilities has not been fully understood and an effective therapy is not available...

  7. STAT3/NF-κB-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer.

    Science.gov (United States)

    Kuo, Wei-Ying; Hwu, Luen; Wu, Chun-Yi; Lee, Jhih-Shian; Chang, Chi-Wei; Liu, Ren-Shyan

    2017-01-01

    Triple-negative breast cancer (TNBC) represents approximately 20% of all breast cancers and appears resistance to conventional cytotoxic chemotherapy, demonstrating a particularly poor prognosis and a significantly worse clinical outcome than other types of cancer. Suicide gene therapy has been used for the in vivo treatment of various solid tumors in recent clinical trials. In tumor microenvironment, STAT3/NF-κB pathways are constitutively activated in stromal cells as well as in cancer stem cells (CSCs). In this study, we have cloned a novel STAT3/NF-κB-based reporter system to drive the expression of herpes simplex virus thymidine kinase (HSV-TK) against breast cancer. Lentiviral vector expressing HSV-TK under the regulation of STAT3/NF-κB fused response element was developed. In this setting, we exploited the constitutive STAT3/NF-κB activation in tumors to achieve higher transgene expression than that driven by a constitutively active CMV promotor in vivo. An orthotropic MDA-MB-231 triple negative breast cancer mouse model was used for evaluating the feasibility of STAT3-NF-κB-TK/GCV suicide gene therapy system. The basal promoter activity of Lenti-CMV-TK and Lenti-STAT3-NF-κB-TK in MDA-MB-231 cells was compared by (3)H-FEAU uptake assay. The Lenti-CMV-TK showed ~5 fold higher (3)H-FEAU uptake then Lenti -STAT3-NF-κB-TK. In clonogenic assay, cells expressing Lenti-CMV-TK were 2-fold more sensitive to GCV than Lenti-STAT3-NF-κB-TK transduced cells. In vitro effect of STAT3-NF-κB-induced transgene expression was determined by 10ng/mL TNF-α induction and confirmed by western blot analysis and DsRedm fluorescent microscopy. In vivo evaluation of therapeutic effect by bioluminescence and [(18)F]FHBG microPET imaging indicated that Lenti-STAT3-NF-κB-TK showed more tumor growth retardation than Lenti-CMV-TK when GCV (20 mg/kg) was administered. The invasiveness and expression of cancer stem cell markers were both decreased after STAT3/NF-κB-regulated HSV

  8. Establishing an ISO 10001-based promise in inpatients care.

    Science.gov (United States)

    Khan, Mohammad Ashiqur Rahman; Karapetrovic, Stanislav

    2015-01-01

    The purpose of this paper is to explore ISO 10001:2007 in planning, designing and developing a customer satisfaction promise (CSP) intended for inpatients care. Through meetings and interviews with research participants, who included a program manager, unit managers and registered nurses, information about potential promises and their implementation was obtained and analyzed. A number of promises were drafted and one was finally selected to be developed as a CSP. Applying the standard required adaptation and novel interpretation. Additionally, ISO 10002:2004 (Clause 7) was used to design the feedback handling activities. A promise initially chosen for development turned out to be difficult to implement, experience that helped in selecting and developing the final promise. Research participants found the ISO 10001-based method useful and comprehensible. This paper presents a specific health care example of how to adapt a standard's guideline in establishing customer promises. The authors show how a promise can be used in alleviating an existing issue (i.e. communication between carers and patients). The learning can be beneficial in various health care settings. To the knowledge, this paper shows the first example of applying ISO 10001:2007 in a health care case. A few activities suggested by the standard are further detailed, and a new activity is introduced. The integrated use of ISO 10001:2007 and 10002:2004 is presented and how one can be "augmented" by the other is demonstrated.

  9. [ISO 15189 accreditation and future perspectives-Chairmen's introductory remarks].

    Science.gov (United States)

    Kayamori, Yuzo; Takagi, Yasushi

    2012-07-01

    Recently, transparency of the organization administration and patient-centered medical care such as patient service and satisfaction have been demanded in the medical field. The role of the clinical laboratory, one of the organizations in the medical field, is to provide quality laboratory data to improve diagnosis and treatment. Other than clinical laboratory facilities, education and the ability of staff to use it, the management of the organization must be appropriate to produce high quality laboratory data. International guidance on how to manage a laboratory is shown in ISO 15189. Clinical laboratories with ISO accreditation in Europe, Australia and Asia are increasing. On the other hand, only 60 institutions (10 national university hospitals, 4 public private university hospitals, 12 private hospitals and 34 referral laboratories) are accredited in Japan. Six speakers spoke at this symposium about the accreditation system of ISO 15189, the experiences of the acquiring institution and the effect of acquisition, as well as the future prospects of ISO 15189. This was a good opportunity to become informed about the current situation of ISO 15189 in Japan and internationally.

  10. Viral safety of C1-inhibitor NF

    NARCIS (Netherlands)

    Terpstra, F. G.; Kleijn, M.; Koenderman, A. H. L.; Over, J.; van Engelenburg, F. A. C.; Schuitemaker, H.; van 't Wout, A. B.

    2007-01-01

    We studied the efficacy of virus reduction by three process steps (polyethylene glycol 4000 (PEG) precipitation, pasteurization, and 15nm virus filtration) in the manufacturing of C1-inhibitor NF. The potential prion removing capacity in this process was estimated based on data from the literature.

  11. NF1 Signal Transduction and Vascular Dysfunction

    Science.gov (United States)

    2015-05-01

    malformations, aneurysms, and hypertension . Consequently there is a markedly elevated risk of cerbrovascular accidents(Friedman et al., 2002). NF1...the endothelium seems to trigger a rapid leukemic crisis . This observation is quite unexpected but consistent with the notion that the endothelial

  12. p100 Deficiency is insufficient for full activation of the alternative NF-κB pathway: TNF cooperates with p52-RelB in target gene transcription.

    Directory of Open Access Journals (Sweden)

    Agnes Lovas

    Full Text Available BACKGROUND: Constitutive activation of the alternative NF-κB pathway leads to marginal zone B cell expansion and disorganized spleen microarchitecture. Furthermore, uncontrolled alternative NF-κB signaling may result in the development and progression of cancer. Here, we focused on the question how does the constitutive alternative NF-κB signaling exert its effects in these malignant processes. METHODOLOGY/PRINCIPAL FINDINGS: To explore the consequences of unrestricted alternative NF-κB activation on genome-wide transcription, we compared gene expression profiles of wild-type and NF-κB2/p100-deficient (p100(-/- primary mouse embryonic fibroblasts (MEFs and spleens. Microarray experiments revealed only 73 differentially regulated genes in p100(-/- vs. wild-type MEFs. Chromatin immunoprecipitation (ChIP assays showed in p100(-/- MEFs direct binding of p52 and RelB to the promoter of the Enpp2 gene encoding ENPP2/Autotaxin, a protein with an important role in lymphocyte homing and cell migration. Gene ontology analysis revealed upregulation of genes with anti-apoptotic/proliferative activity (Enpp2/Atx, Serpina3g, Traf1, Rrad, chemotactic/locomotory activity (Enpp2/Atx, Ccl8, and lymphocyte homing activity (Enpp2/Atx, Cd34. Most importantly, biochemical and gene expression analyses of MEFs and spleen, respectively, indicated a marked crosstalk between classical and alternative NF-κB pathways. CONCLUSIONS/SIGNIFICANCE: Our results show that p100 deficiency alone was insufficient for full induction of genes regulated by the alternative NF-κB pathway. Moreover, alternative NF-κB signaling strongly synergized both in vitro and in vivo with classical NF-κB activation, thereby extending the number of genes under the control of the p100 inhibitor of the alternative NF-κB signaling pathway.

  13. Mosaic type-1 NF1 microdeletions as a cause of both generalized and segmental neurofibromatosis type-1 (NF1)

    OpenAIRE

    Ludwine M. Messiaen; Vogt, Julia; Bengesser, Kathrin; Fu, Chuanhua; Mikhail, Fady; Serra, Eduard; Garcia-Linares, Carles; Cooper, David N; Lázaro, Conxi; Kehrer-Sawatzki, Hildegard

    2011-01-01

    Abstract Mosaicism is an important feature of type-1 neurofibromatosis (NF1) on account of its impact upon both clinical manifestations and transmission risk. Using FISH and MLPA to screen 3500 NF1 patients, we identified 146 individuals harbouring gross NF1 deletions, 14 of whom (9.6%) displayed somatic mosaicism. The high rate of mosaicism in patients with NF1 deletions supports the postulated idea of a direct relationship between the high new mutation rate in this cancer predisp...

  14. Experience of Implementing ISO 15189 Accreditation at a University Laboratory.

    Science.gov (United States)

    Solis-Rouzant, Patricia

    2015-11-01

    The present article summarizes the authors' experience with the implementation of a quality management system based on ISO 17025 and ISO 15189 standards at university laboratories. The accreditation of the analytical procedures at the Universidad Mariano Gálvez represented a challenge due to the unique nature of an educational institution and the difference in nature to the standards implemented. Sample handling and care of the patient were combined to achieve an integrated management system. We explain the development of the management system, the obstacles and benefits of the system and concluding that it is possible to design a management system based on ISO 15189 for the university lab that allowed delivering results assuring technical competence to patient care and welfare.

  15. [ISO 9000: guidelines for a total quality system in health].

    Science.gov (United States)

    Pasini, E; Opasich, C; Scherillo, M

    1998-04-01

    The Italian National Health System has recently been revised and reorganized. In the new scenario, the quality of the service being provided is considered extremely important. The international ISO 9000 standard has made it possible to implement a total-quality management system that can be documented and certified independently by a third party. Several hospitals in Italy and abroad have implemented a total-quality system according to ISO 9000 standards in order to provide a service that can satisfy the expectations of the patients in a controlled and efficient way. This article describes ISO 9000, proposes its application to ensure quality in the structures and organizations involved in the national health system and reports several examples of how the standard has been implemented in hospitals.

  16. Study on the premixed laminar flames of iso-octane

    Science.gov (United States)

    Yang, Bo; Hong, Yan-ji; Xu, Qing-yao; Liu, Yi; Cheng, Qi-sheng; Ding, Xiao-yu

    2015-04-01

    Propagation characteristics of premixed laminar iso-octane flames at atmosphere and equivalence ratios from 0.8 to 1.4 are studied in a constant combustion bomb using a schlieren technique, the laminar burning velocity at different initial pressure, temperature, equivalence ratio are calculated through CHEMKIN program. The experimental and calculation results show that the laminar burning velocity of iso-octane rise with the decreasing of initial pressure and rise with the rising of initial temperature . Only changing the initial temperature or pressure ,the maximum laminar burning velocity of iso-octane were both obtained at equivalence ratio 1.1. Flame stability become weak ,when increased the equivalence ratio. The problem of the chemistry reaction mechanism to predict the laminar burning velocity were analysed.

  17. ISO 9001 benefits and pitfalls: the path to successful certification.

    Science.gov (United States)

    Krause, M S

    1996-09-01

    The lifeblood of a business is developing and commercializing new products with minimum cost and time and maximum quality. Implementation of a quality management system is often used to achieve these goals, and the ISO 9001 standard for a business quality system is rapidly becoming the model of choice. The existence of an ISO-compliant system is a key to meeting the forthcoming regulatory requirements in the European Union and the US Food and Drug Administrations's proposed good management practices. DuPont has demonstrated leadership in the achievement of ISO registration. I describe the path to these successful registrations along with key lessons from the experience. Elements of success are management commitment, adequate resources, education, communication, total organizational involvement, and auditing of system performance. For the system to flourish and provide benefits to the users, sufficient time must be allocated for the organization to change.

  18. Comparison between ASHRAE and ISO thermal transmittance calculation methods

    DEFF Research Database (Denmark)

    Blanusa, Petar; Goss, William P.; Roth, Hartwig

    2007-01-01

    the Heat Transfer and Total Optical Properties of Fenestration Products, Public Review Draft of Standard 142P, American Society of Heating, Refrigerating and Air Conditioning Engineers, Atlanta, 1998]) and in Europe [ISO 10077-2. Thermal Performance of Windows, Doors and Shutters-Calculation of Thermal...... Transmittance-Part 2: Numerical Method for Frames, International Standards Organization, Geneva, 2003]. The two approaches, called the ASHRAE and ISO methods, are different in the way they treat the effect of the glazing spacer on the heat transfer through the frame and the glazing unit near the frame....... The ASHRAE method assumes that the spacer effects both the heat transfer through the frame and the heat transfer through the glazing in an "edge-of glass" region 63.5mm (2.5in.) from the glazing/frame sight line. The ISO method assumes that the additional heat transfer due to the existence of the spacer...

  19. [History of ISO 15189 and its future perspective].

    Science.gov (United States)

    Kawai, Tadashi

    2010-01-01

    ISO 15189, Medical laboratories--Particular requirements for quality and competence was prepared by ISO/TC212, Clinical laboratory testing and in vitro diagnostic test systems, and its first edition published in 2003 and the second edition in 2007. Its purpose is to be used by medical laboratories in developing their quality management systems and assessing their own competence, also for use by accreditation bodies in confirming or recognizing the competence of medical laboratories. Since its publication, it has been recognized officially by the International Laboratory Accreditation Cooperation (ILAC) to be used as the international standard for the accreditation of medical laboratories worldwide. In Japan, the Japanese Committee for Clinical Laboratory Standards (JCCLS) and the Japan Accreditation Board for Conformity Assessment (JAB) cojointly started the accreditation program in August, 2005, and a total of 46 medical laboratories had been accredited by April 2009. In ISO/TC212/WG1 is working on the preparation of its third edition.

  20. [Approach for prevention of medical test malpractice using ISO 15189].

    Science.gov (United States)

    Kayamori, Yuzo; Fujishima, Akiyoshi; Noda, Nozomi; Kurihara, Masako; Tsutsui, Toshiharu; Mochimaru, Tomomi; Ikematsu, Yohko; Monji, Nobuhisa; Ishigaki, Takuya; Aoki, Kanae; Ushinohama, Sayaka; Nishioka, Shoko; Egashira, Sadaomi; Kang, Dongchon

    2010-08-01

    With the increasing need for medical laboratory data, the importance of the medical laboratory in medical care has grown exponentially. Therefore, it is necessary that the medical laboratory provide the doctor and the patient receiving medical care with accurate, precise, and reliable medical laboratory data. Quality assurance and safety management in the medical laboratory are necessary to prevent medical test malpractice, which would lead to a medical accident, and to manage the overall processes required to provide high quality medical laboratory data. ISO 15189 is an international standard known as an organizational management tool for medical laboratories. Our medical laboratory acquired this ISO in March 2005 and we utilized PDCA cycle as required by the international standard in order to establish appropriate safety management and crisis control. We introduced practices for quality improvement and prevention of medical test malpractice proposed by the ISO committee of our medical laboratory and describe these practices in this report.

  1. Functional interactions between an atypical NF-κB site from the rat CYP2B1 promoter and the transcriptional repressor RBP-Jκ/CBF1

    OpenAIRE

    Lee, Sang Hyun; Wang, Xiao-Li; DeJong, Jeff

    2000-01-01

    The phenobarbital-inducible rat cytochrome P450 (CYP) 2B1 and 2B2 proteins are encoded by homologous genes whose promoters contain a mammalian-apparent long terminal repeat retrotransposon (MaLR). An NF-κB-like site within the MaLR forms multiple protein–DNA complexes with rat liver and HeLa cell nuclear extracts. Using antibody supershift assays, we have identified these complexes as NF-κB and RPB-Jκ/CBF1. Competition assays using a series of single site mutant oligonucleotides reveal that t...

  2. Progress report on the management of the NEA ISOE system

    Energy Technology Data Exchange (ETDEWEB)

    Lazo, E. [OECD Nuclear Energy Agency, Issy-les-Moulineaux (France)

    1995-03-01

    The Information System on Occupational Exposure (ISOE) was launched by the Organization for Economic Cooperation and Development (OECD), Nuclear Energy Agency (NEA) on 1 January, 1992, to facilitate the communication of dosimetric and ALARA implementation data among nuclear utilities around the world. After two years of operation the System has become a mature interactive network for transfer of data and experience. Currently, 37 utilities from 12 countries, representing 289 power plants, and 12 national regulatory authorities participate in ISOE. Agreements for cooperation also exist between the NEA and the Commission of the European Communities (CEC), and the Paris Center of the WOrld Association of Nuclear Operators (WANO-PC). In addition, the International Atomic Energy Agency (IAEA) is acting as a co-sponsor of ISOE for the participation of non-NEA member countries. Three Regional Technical Centres, Europe, Asia, and Non-NEA member countries, serve to administer the system. The ISOE Network is comprised of three data bases and a communications network at several levels. The three ISOE data bases include the following types of information: NEA1 - annual plant dosimetric information; NEA2 - plant operational characteristics for dose and dose rate reduction; and NEA3 - job specific ALARA practices and experiences. The ISOE communications network has matured greatly during 1992 and 1993. In addition to having access to the above mentioned data bases, participants may now solicit information on new subjects, through the Technical Centres, from all other participants on a real-time basis. Information Sheets on these studies are produced for distribution to all participants. In addition, Topical Reports on areas of interest are produced, and Topical Meetings are held annually.

  3. ISO 14000: Review and guidelines for the photovoltaic industry

    Energy Technology Data Exchange (ETDEWEB)

    DePhillips, M.P.

    1997-08-01

    The International Organization for Standardization (ISO) has released and is in the process of ratifying a new set of environmental management system standards, ISO 14000. These voluntary international standards will have a significant impact on how companies throughout the international community manage their environmental programs. Initially this impact will result in increased international trade opportunities due to the anticipated customer requirement for suppliers to demonstrate certification. Other potential impacts will benefit both companies engaged in international commerce and companies with a domestic focus. These would include installing a cohesive system to manage environmental matters, participation within a new regulatory paradigm, and adhering to a potentially new set of legal standards. The wide range of potential environmental, commercial, legal and regulatory benefits offered by ISO 14000 offer most companies significant reason to explore the possibilities of implementing an environmental management system. Organizations will be able to anticipate and meet growing environmental expectations and to ensure ongoing compliance with national and international requirements as well as satisfy the growing demands of customers, stakeholders and the interested public. As an emerging industry with significant international ties, the photovoltaic (PV) community will be effected from these standards if they do become globally adopted. In fact some major players in the PV industry have recently received certification to ISO 9000. This again demonstrates a trend within the industry to move toward a uniform system approach to management. This paper will expose interested parties within the PV industry to the details of ISO 14000 and will assist decision makers assess the various possibilities for implementation certification of ISO 14000 for an individual facility.

  4. Mednarodni standardi - veličine in enote (ISO 31-0 do 31-13): International standards - quantities and units (ISO 31-0 to 31-13):

    OpenAIRE

    Glavič, Peter

    2002-01-01

    In this paper the international standards ISO 31 (Quantities and units) are presented with the following parts: ISO 31-0 (General principles), ISO 31-1 (Space and time), ISO 31-2 (Periodic and related phenomena), ISO 31-3 (Mechanics), ISO 31-4 (Heat), ISO 31-5 (Electricity and magnetism), ISO 31-8 (Physical chemistry and molecular physics), ISO 31-12 (Characteristic numbers)and others. The emphasis is given on the basic principles, which is important for writing of reports, presentations, art...

  5. ISO 14001:2015: An improved tool for sustainability

    Directory of Open Access Journals (Sweden)

    Luis Miguel Ciravegna Martins da Fonseca

    2015-01-01

    Full Text Available Purpose: ISO 14001 is an International Standard of worldwide acceptance based on the concept that better environmental performance can be achieved when environmental aspects are systematically identified and managed giving a major contribution to Sustainability, through pollution prevention, improved environmental performance and complying with applicable laws.This paper aims to discuss the Sustainability approach through the use of Environmental Management Standards and the current process of ISO 14001:2015 revision. Design/methodology/approach: Revisiting the concept of Sustainability, the status of the revision process of ISO 14001:2015 and its expected outcomes are discussed. Findings and Originality/value: It is one of the first attempts to analyze, within the Sustainability framework, the status of the revision of the future ISO 14001:2015 International Standard that will respond to the latest trends and is expected to further improve Environmental performance by impacting the work of many People and Organizations worldwide and also ensuring it is compatible with other management system standards such as ISO 9001. Research limitations/implications: Since the revision standard is not yet finished some changes might still happen and some of the remarks expressed in this paper might not be consensual within the Environmental Community. Practical implications: There will be a 3 year transition process for the ISO 14001:2015 certification and major benefits like Quality Management Systems with less emphasis on documentation and new/reinforced approaches as consideration of organizational context and (relevant Stakeholders, risk based thinking, life cycle perspective and more emphasis on improving environmental performance. Social implications: The ISO 14001:2015 revision will have major impacts on the more than 300.000 worldwide certified organizations and on the many professionals that work with, hopefully Originality/value: Although 14001

  6. Managing your library and its quality the ISO 9001 way

    CERN Document Server

    Balagué, Núria

    2011-01-01

    This book, divided into two parts, provides an introduction to the quality management issues and gives a general overview to the use of ISO 9001 in the library environment. The second part presents the main features of ISO 9001:2008 with practical comments and examples on how to implement its clauses in libraries. Whether in the public or in the private sector, libraries can be seen as service organisations: they act in very dynamic environments where users are increasingly demanding new types of services. Thus the adoption of a quality management system helps each library in meeting the needs

  7. Revision of the ISO and EN radiation sterilization standards

    DEFF Research Database (Denmark)

    Miller, A.; Hansen, J.

    2002-01-01

    The radiation sterilization standards, ISO 11137 and EN 552, are now being revised under "ISO lead", with the aim of producing only one international standard, although in four parts: (1) requirements. (2) dose-setting methods, (3) dose-substantiation methods and (4) dosimetry. Several aspects...... of the old standards that have caused problems, when they were used in practice, are addressed in the revision. Input from users of the standards is necessary in order that the standards be developed as useful tools in the documentation of radiation sterilization. (C) 2002 Elsevier Science Ltd. All rights...

  8. Imagery metadata development based on ISO/TC 211 standards

    Directory of Open Access Journals (Sweden)

    Rong Xie

    2007-04-01

    Full Text Available This paper reviews the present status and major problems of the existing ISO standards related to imagery metadata. An imagery metadata model is proposed to facilitate the development of imagery metadata on the basis of conformance to these standards and combination with other ISO standards related to imagery. The model presents an integrated metadata structure and content description for any imagery data for finding data and data integration. Using the application of satellite data integration in CEOP as an example, satellite imagery metadata is developed, and the resulting satellite metadata list is given.

  9. Characterization and robust filtering of multifunctional surfaces using ISO standards

    DEFF Research Database (Denmark)

    Friis, Kasper Storgaard; Godi, Alessandro; De Chiffre, Leonardo

    2011-01-01

    to the multi-process production method involved. A series of MUFU surfaces were characterized by using the ISO 13565 standard for stratified surfaces and it is shown that the standard in some cases is inadequate for the characterization of a MUFU surface. To improve the filtering of MUFU surfaces, the robust...... Gaussian regression filtering technique described in ISO 16610-31 is analyzed and discussed. By slight modifications it is shown how the robust Gaussian regression filter can be applied to remove the form and find a suitable reference surface for further characterization of the MUFU surfaces...

  10. U-value measurements on ISO round Robin window

    DEFF Research Database (Denmark)

    Duer, Karsten

    1997-01-01

    9 laboratories in 6 european and 2 north american countries have participated in a round robin U-value test of a standard facade window. The objective of the round robin test was to verify that the ISO/CEN proposals for U-value measurement standard is robust.......9 laboratories in 6 european and 2 north american countries have participated in a round robin U-value test of a standard facade window. The objective of the round robin test was to verify that the ISO/CEN proposals for U-value measurement standard is robust....

  11. ISO 15189:2003--quality management, evaluation and continual improvement.

    Science.gov (United States)

    Burnett, David

    2006-01-01

    The requirements of ISO 15189:2003 are discussed in the context of a process- and outcome-based quality management model in which the user's needs are the central focus. The requirements of ISO 15189:2003 are examined in terms of organisation and a quality management system, stressing the importance of evidence, document control, and control of records and clinical material. Examples are provided from the areas of resource management, and pre-examination, examination and post-examination processes. In the final section the importance of evaluation and continual improvement is presented in relation to internal audit and external assessment, non-conformity, corrective and preventative action and management review.

  12. Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells.

    Science.gov (United States)

    Assar, Emelia A; Vidalle, Magdalena Castellano; Chopra, Mridula; Hafizi, Sassan

    2016-07-01

    We studied the effect of the potent dietary antioxidant lycopene on multiple points along the nuclear factor kappa B (NF-κB) signaling pathway in prostate and breast cancer cells. Lycopene significantly inhibited prostate and breast cancer cell growth at physiologically relevant concentrations of ≥1.25 μM. Similar concentrations also caused a 30-40 % reduction in inhibitor of kappa B (IκB) phosphorylation in the cells, as determined by western blotting. Furthermore, the same degree of inhibition by lycopene was observed for NF-κB transcriptional activity, as determined by reporter gene assay. Concomitant with this, immunofluorescence staining of lycopene-treated cells showed a significant suppression (≥25 %) of TNF-induced NF-κB p65 subunit nuclear translocation. Further probing of lycopene's effects on upstream elements of the NF-κB pathway showed a 25 % inhibition of both activity of recombinant IκB kinase β (IKKβ) kinase in a cell-free in vitro assay, as well as activity of IKKβ immunoprecipitated from MDA-MB-231 cells treated with lycopene. In conclusion, the anticancer properties of lycopene may occur through inhibition of the NF-κB signaling pathway, beginning at the early stage of cytoplasmic IKK kinase activity, which then leads to reduced NF-κB-responsive gene regulation. Furthermore, these effects in cancer cells were observed at concentrations of lycopene that are relevant and achievable in vivo.

  13. Human T-cell leukemia virus type 1 bZIP factor selectively suppresses the classical pathway of NF-kappaB.

    Science.gov (United States)

    Zhao, Tiejun; Yasunaga, Jun-ichirou; Satou, Yorifumi; Nakao, Mitsuyoshi; Takahashi, Masahiko; Fujii, Masahiro; Matsuoka, Masao

    2009-03-19

    Adult T-cell leukemia (ATL) is a highly aggressive T-cell malignancy caused by human T-cell leukemia virus type 1 (HTLV-1). The activation of NF-kappaB by Tax has been reported to play a crucial role in HTLV-1-induced transformation. The HTLV-1 bZIP factor (HBZ), which is encoded by an mRNA of the opposite polarity of the viral genomic RNA, is involved in both T cell proliferation and suppression of Tax-mediated viral gene transcription, suggesting that HBZ cooperates closely with Tax. In the present study, we observed that HBZ specifically suppressed NF-kappaB-driven transcription mediated by p65 (the classical pathway) without inhibiting the alternative NF-kappaB signaling pathway. In an immunoprecipitation assay, HBZ bound to p65 and diminished the DNA binding capacity of p65. In addition, HBZ induced p65 degradation through increasing the expression of the PDLIM2 gene, which encodes a ubiquitin E3 ligase for p65. Finally, HBZ actually repressed the transcription of some classical NF-kappaB target genes, such as IL-8, IL2RA, IRF4, VCAM-1, and VEGF. Selective suppression of the classical NF-kappaB pathway by HBZ renders the alternative NF-kappaB pathway predominant after activation of NF-kappaB by Tax or other stimuli, which might be critical for oncogenesis.

  14. A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F2α (8-iso-PGF2α) including pediatric reference interval.

    Science.gov (United States)

    Xiao, Yi; Fu, Xiaowei; Pattengale, Paul; Dien Bard, Jennifer; Xu, Yan-Kang; O'Gorman, Maurice R

    2016-09-01

    Oxidative stress has been implicated in numerous diseases, including arthritis, atherosclerosis, Alzheimer's disease, cancer, diabetes, hypertension, and inflammation. 8-iso-prostaglandin F2α, a member of the F2 isoprostane family, has been well-accepted as a valuable biomarker for the assessment of oxidative stress. We report the development and validation of an ultra-sensitive LC-MS/MS assay for urinary 8-iso-PGF2α measurements in pediatric population. The assay was linear from 0.024 to 20nmol/l (R(2)=0.99). Recoveries were above 85% and matrix effects were below 5%. The variability was determined at nmol/l concentration: the intra-day variability (%CV) ranged from 3.9% to 4.5% (n=20); and the inter-day variability ranged from 4.3% to 5.7% (n=20). The accuracy of our laboratory developed test was evaluated with a clinical reference laboratory (n=39), and a correlation coefficient of 0.9257 was observed. Reference interval were established to be <0.5ng/mg creatinine in a group of pediatric population (2months-18years, n=123). The precision of the assay will allow for accurate assessment of oxidative stress, and is acceptable for patient testing, particularly in pediatric population. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. A Fluorescence-Based High-Throughput Coupled Enzymatic Assay for Quantitation of Isoaspartate in Proteins and Peptides.

    Science.gov (United States)

    Puri, Aastha; Quan, Yong; Narang, Ajit S; Adams, Monica; Gandhi, Rajesh; Nashine, Vishal C

    2017-04-01

    Formation of isoaspartate (IsoAsp) from spontaneous asparagine (Asn) deamidation or aspartate (Asp) isomerization is one of the most common non-enzymatic pathways of chemical degradation of protein and peptide pharmaceuticals. Rapid quantitation of IsoAsp formation can enable rank-ordering of potential drug candidates, mutants, and formulations as well as support shelf life prediction and stability requirements. A coupled enzymatic fluorescence-based IsoAsp assay (CEFIA) was developed as a high-throughput method for quantitation of IsoAsp in peptides and proteins. In this note, application of this method to two therapeutic candidate proteins with distinct structural scaffolds is described. In addition, the results obtained with this method are compared to those from conventional assays.

  16. Role of Merlin/NF2 inactivation in tumor biology.

    Science.gov (United States)

    Petrilli, A M; Fernández-Valle, C

    2016-02-04

    Merlin (Moesin-ezrin-radixin-like protein, also known as schwannomin) is a tumor suppressor protein encoded by the neurofibromatosis type 2 gene NF2. Loss of function mutations or deletions in NF2 cause neurofibromatosis type 2 (NF2), a multiple tumor forming disease of the nervous system. NF2 is characterized by the development of bilateral vestibular schwannomas. Patients with NF2 can also develop schwannomas on other cranial and peripheral nerves, as well as meningiomas and ependymomas. The only potential treatment is surgery/radiosurgery, which often results in loss of function of the involved nerve. There is an urgent need for chemotherapies that slow or eliminate tumors and prevent their formation in NF2 patients. Interestingly NF2 mutations and merlin inactivation also occur in spontaneous schwannomas and meningiomas, as well as other types of cancer including mesothelioma, glioma multiforme, breast, colorectal, skin, clear cell renal cell carcinoma, hepatic and prostate cancer. Except for malignant mesotheliomas, the role of NF2 mutation or inactivation has not received much attention in cancer, and NF2 might be relevant for prognosis and future chemotherapeutic approaches. This review discusses the influence of merlin loss of function in NF2-related tumors and common human cancers. We also discuss the NF2 gene status and merlin signaling pathways affected in the different tumor types and the molecular mechanisms that lead to tumorigenesis, progression and pharmacological resistance.

  17. Natural chalcones as dual inhibitors of HDACs and NF-κB

    Science.gov (United States)

    ORLIKOVA, B.; SCHNEKENBURGER, M.; ZLOH, M.; GOLAIS, F.; DIEDERICH, M.; TASDEMIR, D.

    2012-01-01

    Histone deacetylase enzymes (HDACs) are emerging as a promising biological target for cancer and inflammation. Using a fluorescence assay, we tested the in vitro HDAC inhibitory activity of twenty-one natural chalcones, a widespread group of natural products with well-known anti-inflammatory and antitumor effects. Since HDACs regulate the expression of the transcription factor NF-κB, we also evaluated the inhibitory potential of the compounds on NF-κB activation. Only four chalcones, isoliquiritigenin (no. 10), butein (no. 12), homobutein (no. 15) and the glycoside marein (no. 21) showed HDAC inhibitory activity with IC50 values of 60–190 μM, whereas a number of compounds inhibited TNFα-induced NF-κB activation with IC50 values in the range of 8–41 μM. Interestingly, three chalcones (nos. 10, 12 and 15) inhibited both TNFα-induced NF-κB activity and total HDAC activity of classes I, II and IV. Molecular modeling and docking studies were performed to shed light into dual activity and to draw structure-activity relationships among chalcones (nos. 1–21). To the best of our knowledge this is the first study that provides evidence for HDACs as potential drug targets for natural chalcones. The dual inhibitory potential of the selected chalcones on NF-κB and HDACs was investigated for the first time. This study demonstrates that chalcones can serve as lead compounds in the development of dual inhibitors against both targets in the treatment of inflammation and cancer. PMID:22710558

  18. Downregulation of SAFB Sustains the NF-κB Pathway by Targeting TAK1 during the Progression of Colorectal Cancer.

    Science.gov (United States)

    Jiao, Hong-Li; Ye, Ya-Ping; Yang, Run-Wei; Sun, Hui-Ying; Wang, Shu-Yang; Wang, Yong-Xia; Xiao, Zhi-Yuan; He, Liu-Qing; Cai, Juan-Juan; Wei, Wen-Ting; Chen, Yan-Ru; Gu, Chun-Cai; Cai, Yue-Long; Hu, Yun-Teng; Lai, Qiu-Hua; Qiu, Jun-Feng; Liang, Li; Cao, Guang-Wen; Liao, Wen-Ting; Ding, Yan-Qing

    2017-11-15

    Purpose: To investigate the role and the underlying mechanism of scaffold attachment factor B (SAFB) in the progression of colorectal cancer (CRC).Experimental Design: SAFB expression was analyzed in the Cancer Outlier Profile Analysis of Oncomine and in 175 paraffin-embedded archived CRC tissues. Gene Ontology analyses were performed to explore the mechanism of SAFB in CRC progression. Western blot, RT-PCR, luciferase assay, and chromatin immunoprecipitation (ChIP) were used to detect the regulation of transforming growth factor-β-activated kinase 1 (TAK1) and NF-κB signaling by SAFB The role of SAFB in invasion, metastasis, and angiogenesis was investigated using in vitro and in vivo assays. The relationship between SAFB and TAK1 was analyzed in CRC tissues.Results: SAFB was downregulated in CRC tissues, and low expression of SAFB was significantly associated with an aggressive phenotype and poorer survival of CRC patients. The downregulation of SAFB activated NF-κB signaling by targeting the TAK1 promoter. Ectopic expression of SAFB inhibited the development of aggressive features and metastasis of CRC cells both in vitro and in vivo The overexpression of TAK1 could rescue the aggressive features in SAFB-overexpressed cells. Furthermore, the expression of SAFB in CRC tissues was negatively correlated with the expression of TAK1- and NF-κB-related genes.Conclusions: Our results show that SAFB regulated the activity of NF-κB signaling in CRC by targeting TAK1 This novel mechanism provides a comprehensive understanding of both SAFB and the NF-κB signaling pathway in the progression of CRC and indicates that the SAFB-TAK1-NF-κB axis is a potential target for early therapeutic intervention in CRC progression. Clin Cancer Res; 23(22); 7108-18. ©2017 AACR. ©2017 American Association for Cancer Research.

  19. Cytochrome P450 (CYP) 2J2 gene transfection attenuates MMP-9 via inhibition of NF-kappabeta in hyperhomocysteinemia.

    Science.gov (United States)

    Moshal, Karni S; Zeldin, Darryl C; Sithu, Srinivas D; Sen, Utpal; Tyagi, Neetu; Kumar, Munish; Hughes, William M; Metreveli, Naira; Rosenberger, Dorothea S E; Singh, Mahavir; Vacek, Thomas P; Rodriguez, Walter E; Ayotunde, Adeagbo; Tyagi, Suresh C

    2008-06-01

    Hyperhomocysteinemia (HHcy) is associated with atherosclerotic events involving the modulation of arachidonic acid (AA) metabolism and the activation of matrix metalloproteinase-9 (MMP-9). Cytochrome P450 (CYP) epoxygenase-2J2 (CYP2J2) is abundant in the heart endothelium, and its AA metabolites epoxyeicosatrienoic acids (EETs) mitigates inflammation through NF-kappabeta. However, the underlying molecular mechanisms for MMP-9 regulation by CYP2J2 in HHcy remain obscure. We sought to determine the molecular mechanisms by which P450 epoxygenase gene transfection or EETs supplementation attenuate homocysteine (Hcy)-induced MMP-9 activation. CYP2J2 was over-expressed in mouse aortic endothelial cells (MAECs) by transfection with the pcDNA3.1/CYP2J2 vector. The effects of P450 epoxygenase transfection or exogenous supplementation of EETs on NF-kappabeta-mediated MMP-9 regulation were evaluated using Western blot, in-gel gelatin zymography, electromobility shift assay, immunocytochemistry. The result suggested that Hcy downregulated CYP2J2 protein expression and dephosphorylated PI3K-dependent AKT signal. Hcy induced the nuclear translocation of NF-kappabeta via downregulation of IKbetaalpha (endogenous cytoplasmic inhibitor of NF-kappabeta). Hcy induced MMP-9 activation by increasing NF-kappabeta-DNA binding. Moreover, P450 epoxygenase transfection or exogenous addition of 8,9-EET phosphorylated the AKT and attenuated Hcy-induced MMP-9 activation. This occurred, in part, by the inhibition of NF-kappabeta nuclear translocation, NF-kappabeta-DNA binding and activation of IKbetaalpha. The study unequivocally suggested the pivotal role of EETs in the modulation of Hcy/MMP-9 signal. (c) 2008 Wiley-Liss, Inc.

  20. Requerimientos establecidos por las normas ISO para papeles permanentes Established requirements for ISO standards for permanent papers

    Directory of Open Access Journals (Sweden)

    Juan Facundo Araujo

    2011-06-01

    Full Text Available El patrimonio documental nacional e internacional se encuentra en su gran mayoría en soporte papel. A partir de la industrialización en la manufactura de dicho material se ha producido papel con niveles de acidez, que sumado a otros factores, ha sido causante del deterioro de este tipo de patrimonio documental. En las últimas décadas, con la incorporación de nuevos procesos dentro de la producción papelera se ha resuelto en parte dicha problemática. El organismo internacional de normalización ISO ha redactado dos normas al respecto: la norma ISO 9706 e ISO 11108. Con la difusión y recomendación de uso del papel permanente para la conservación del patrimonio documental a largo plazo, se hace imprescindible conocer dicha normativa e informarse al respecto. En el presente artículo se describen las variables en la fabricación del papel que influyen en la permanencia de los papeles. También se determinan los conceptos y antecedentes que rodean ambas normas ISO, así como las características principales y partes que componen las mismas. Como anexo se consigna un glosario con los principales términos técnicos utilizados.Most of the national and international documentary heritage are on paper. From industrialization in the manufacture of such material, paper with acidity levels has been produced which, together with other factors, has been causing the deterioration of this type of documentary heritage. In recent decades, with the incorporation of new paper production processes this problem has been partly solved. The international standards organism ISO has drafted two rules in this connection: ISO 9706 and ISO 11108. With the spread and recommendation for the use of permanent paper for preservation of documentary heritage in the long term, it is essential to know the standards and be well informed. This article describes the variables in the manufacture of paper that influence the permanence of the papers. It also identifies the

  1. The NF1 somatic mutational landscape in sporadic human cancers.

    Science.gov (United States)

    Philpott, Charlotte; Tovell, Hannah; Frayling, Ian M; Cooper, David N; Upadhyaya, Meena

    2017-06-21

    Neurofibromatosis type 1 (NF1: Online Mendelian Inheritance in Man (OMIM) #162200) is an autosomal dominantly inherited tumour predisposition syndrome. Heritable constitutional mutations in the NF1 gene result in dysregulation of the RAS/MAPK pathway and are causative of NF1. The major known function of the NF1 gene product neurofibromin is to downregulate RAS. NF1 exhibits variable clinical expression and is characterized by benign cutaneous lesions including neurofibromas and café-au-lait macules, as well as a predisposition to various types of malignancy, such as breast cancer and leukaemia. However, acquired somatic mutations in NF1 are also found in a wide variety of malignant neoplasms that are not associated with NF1. Capitalizing upon the availability of next-generation sequencing data from cancer genomes and exomes, we review current knowledge of somatic NF1 mutations in a wide variety of tumours occurring at a number of different sites: breast, colorectum, urothelium, lung, ovary, skin, brain and neuroendocrine tissues, as well as leukaemias, in an attempt to understand their broader role and significance, and with a view ultimately to exploiting this in a diagnostic and therapeutic context. As neurofibromin activity is a key to regulating the RAS/MAPK pathway, NF1 mutations are important in the acquisition of drug resistance, to BRAF, EGFR inhibitors, tamoxifen and retinoic acid in melanoma, lung and breast cancers and neuroblastoma. Other curiosities are observed, such as a high rate of somatic NF1 mutation in cutaneous melanoma, lung cancer, ovarian carcinoma and glioblastoma which are not usually associated with neurofibromatosis type 1. Somatic NF1 mutations may be critical drivers in multiple cancers. The mutational landscape of somatic NF1 mutations should provide novel insights into our understanding of the pathophysiology of cancer. The identification of high frequency of somatic NF1 mutations in sporadic tumours indicates that neurofibromin is

  2. In vivo circadian oscillation of dCREB2 and NF-κB activity in the Drosophila nervous system.

    Directory of Open Access Journals (Sweden)

    Anne K Tanenhaus

    Full Text Available cAMP response element-binding protein (CREB and nuclear factor kappa-B (NF-κB are two ubiquitous transcription factors involved in a wide number of cellular processes, including the circadian system. Many previous studies on these factors use cellular assays that provide limited information on circadian activity or anatomical specificity. The ability to study transcription factors in defined tissue within intact animals will help to bridge the gap between cellular and in vivo data. We have used the GAL4-UAS and FLP-FRT systems to gain spatial control over reporter gene expression. Using a luciferase-based reporter, we show in vivo that Drosophila dCREB2- and NF-κB-mediated transcription oscillates in neuronal cells, glia, and in the mushroom body, a higher-order brain center in flies. This oscillation is under circadian control, cycling with a 24-hour rhythm, under both light-dark and dark-dark conditions. In light-light conditions, dCREB2 and NF-κB reporter flies exhibit a suppression of rhythmic activity. Furthermore, neuronal cycling of dCREB2 and NF-κB activity are modulated in period mutant flies, indicating these oscillations are controlled through the central clock. This study shows for the first time region-specific circadian oscillation of dCREB2/NF-κB activity in the Drosophila nervous system.

  3. Ctenopharyngodon idella p53 mediates between NF-κB and PKR at the transcriptional level.

    Science.gov (United States)

    Huang, Qingli; Xie, Dingkun; Mao, Huiling; Wang, Haizhou; Wu, Zhen; Huang, Keyi; Wan, Yiqi; Xu, Qun; Hu, Chengyu

    2017-10-01

    p53, NF-κB and PKR are well-known to be involved in antiviral response. Although p53 has been reported in fish, its role in the regulation of NF-κB and PKR is not well understood. Here, we cloned and characterized the full length of cDNA sequence of grass carp (Ctenopharyngodon idella) p53 (Cip53) and its promoter sequence. The full length cDNA of Cip53 was 1879 bp with an ORF of 1116 bp encoding a polypeptide of 371 amino acids. Phylogenetic tree analysis revealed that Cip53 shares high homology with Dario rerio p53 (Drp53). Similar to those of Cip65 and CiPKR, the expression of Cip53 in CIK cells was significantly up-regulated after stimulation with poly I:C. To further understand the roles of fish p53 in the transcriptional control of NF-κB and PKR, Cip53 and Cip65 were expressed in E. coli BL21 and purified by affinity chromatography with the Ni-NTA His-Bind resin. In vitro, gel mobility shift assays demonstrated that the high affinity interaction between Cip65 and Cip53 promoter. Similarly, Cip53 bound to CiPKR promoter with high affinity. Dual-luciferase reporter assays showed that Cip65 activated Cip53 promoter and Cip53 activated CiPKR promoter, respectively. In addition, the role of p53 in p65-p53-PKR transcription pathway was explored. When Cip53 was knockdown in CIK cells, the mRNA levels of Cip65 and CiPKR were decreased. Taken together, p53 may play pivotal roles in transcription pathway of NF-κB and PKR in fish. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Re-engineering caused by ISO-9000 certification

    DEFF Research Database (Denmark)

    Hvam, Lars; Nielsen, Anders Paarup; Bjarnø, Ole-Christian

    1997-01-01

    Based on a project performed at a medium-sized producer of medical utensils, reviews some of the problems which the company experienced in connection with the system built up during ISO 9001 certification, and the re-engineering efforts which were performed in order to relieve these problems...

  5. First Results of ISO-SWS Grating Observations of Saturn

    NARCIS (Netherlands)

    de Graauw, Th.; Encrenaz, Th.; Schaeidt, S.; Lellouch, E.; Feuchtgruber, H.; Beintema, D. A.; Bezard, B.; Drossart, P.; Griffin, M.; Heras, A.; Kessler, M.; Leech, K.; Morris, A.; Roelfsema, P. R.; Roos-Serote, M.; Salama, A.; Vandenbussche, B.; Valentijn, E. A.; Davies, G. R.; Naylor, D. A.

    1996-01-01

    The spectrum of Saturn has been recorded between 2.3 and 15 mu m, on June 13, 1996, with the grating mode of the Short-Wavelength Spectrometer of ISO (Infrared Space Observatory). The resolving power is about 1500 and the sensitivity is better than 1 Jy. As compared to Jupiter, the spectrum of

  6. Implementation of ISO 50001 Energy Management System in Sports Stadia

    Directory of Open Access Journals (Sweden)

    Aidan Byrne

    2014-12-01

    Full Text Available Many modern sports stadia around the world consume large amounts of energy during their dayto- day operations. With the cost of this energy constantly on the rise, the challenge of managing this uncontrolled cost has become increasingly more important for the successful and sustainable operation of these facilities. It is essential that some form of energy management system be embraced by these sports stadia. This paper is a case study on Aviva Stadium’s recent implementation of the ISO 50001 Energy Management System. The authors identify the potential challenges and benefits of implementing the ISO 50001 Energy Management System in sports stadia. Final certification to the standard came on the 25th of September 2013 making Aviva Stadium the first stadium in the world to have achieved thirdparty certification to the ISO 50001 standard. This paper can act as a guide for other stadia wishing to adapt ISO 50001 to their venue, especially since it resulted in a €1 million energy cost avoidance over a three-year period.

  7. THE ISO 14001 ENVIRONMENTAL MANAGEMENT SYSTEMS AS A ...

    African Journals Online (AJOL)

    THE ISO 14001 ENVIRONMENTAL MANAGEMENT SYSTEMS AS A MOTIVATION TOWARDS SUSTAINABLE DEVELOPMENT. ... Results obtained show that both plants affirmed that they are adequately monitoring their key significant aspects and they are complying with environmental laws and regulations. The number ...

  8. ISO-SWS spectra of OH/IR stars

    NARCIS (Netherlands)

    Cami, J; De Jong, T; Justtannont, K; Yamamura, [No Value; Waters, LBFM

    1998-01-01

    We present the spectra of 5 OH/IR stars observed with the Short Wavelength Spectrometer (SWS) on board the Infrared Space Observatory (ISO). The spectra are dominated by dust features, both in the amorphous and crystalline state and illustrate the influence of the mass loss rate on the observed

  9. ISO/IEC 38500 the IT governance standard

    CERN Document Server

    Calder, Alan

    2008-01-01

    The emergence of ISO/IEC 38500 - the international standard for the corporate governance of information and communication technology - puts boards around the world in a position from which they can take effective action to apply core governance principles to their information and communication technology.

  10. Certification Board and Effectiveness Audit in ISO14001

    OpenAIRE

    美濃, 英雄; 丸谷, 一耕; 中村, 修

    2010-01-01

    The Certification Board is functioning for diffusion of ISO14001 that is a part of the environmental management system. Recently, the Certification Board is shifting from the conventional conformity audit to the effectiveness audit. Therefore, the definition of the effectiveness audit and the reality were investigated.

  11. ISO19770-1:2012 SAM process guidance

    CERN Document Server

    Canavan, Rory

    2012-01-01

    The pocket guide offers a concise summary of the principles of software asset management as conveyed by ISO 19770-1: 2012, and provides advice and guidance on how to kick-start your own SAM programme - something the Standard alone doesn't offer.

  12. ISO 9001:2000--setting the standard for quality management.

    Science.gov (United States)

    Edelstein, M E

    2001-04-01

    How will you approach the next quality management initiative? This article offers an introduction to the ISO 9001:2000 quality management system standards that can be applied to the HIM industry and how these standards can improve your department's policies and procedures.

  13. [ISO 9001-2000 certification for refractive laser treatment].

    Science.gov (United States)

    Lebuisson, D A; Arson, B; Abenhaïm, A; Albou-Ganem, C; Assouline, M; Chong Sit, D; Danan, A; Montefiore, G; Montin, J-F; Nguyen-Khoa, J-L; Pietrini, D; Saragoussi, J-J; Trong, Th; Amar, R

    2005-05-01

    To obtain ISO 9001-2000 certification for laser corneal refractive treatment, never before sought in Europe. The consulting firm Veritas led the certification process with the clinic's staff manager. This ISO norm is dedicated to the implementation of a quality management system. We assessed and optimized all necessary resources, evaluating customer satisfaction using patient and referring-physician surveys. We started quality rounds including surgeons, nurses, and technicians. Based on this preparation, we redefined and explained all processes including staff responsibilities and necessary resources in the quality manual. The procedure lasted 14 months with substantial involvement on the part of the management. Unconditional ISO 9001-2000 certification was granted by the independent audit firm, BVQI, in december 2003 for refractive laser treatment. The 2000 version of the new ISO 9001-2000 seeks to meet the demand for improving health care delivery in this field, most particularly by establishing a clear procedural orientation. Such certification enhances team work, stabilizes methodologies, and reinforces cohesion and self-audit. Patients notice that the center follows a consistent quality policy and are assured that the clinic respects rules and regulations. Certification is an advantageous alternative when accreditation cannot be considered. Our article discusses the steps taken in upper management, quality management, procedural guidance, as well as customer and staff counselling. It also discusses the project's cost/benefit ratio for the organization.

  14. The ISO 14001 Environmental Management Systems as a Motivation

    African Journals Online (AJOL)

    user1

    the environmental, social and economical performance of Mauritian industries. ▫. Reveal whether the introduction of ISO 14001 in an organization can be used as an active tool for promoting sustainable development. 4.0 METHODOLOGY OF STUDY. The methodology gives an overview of the method that has been adopted.

  15. Upgrading your Quality Management System to ISO 9000 Standards ...

    African Journals Online (AJOL)

    Upgrading your Quality Management System to ISO 9000 Standards. Eugene Okolie. Abstract. No Abstract Available LBS Management Review Vol.5(2) 2000: 73-81. Full Text: EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Article Metrics. Metrics Loading ... Metrics powered by ...

  16. ISO 9000 and the total quality management models

    OpenAIRE

    Pacios Lozano, Ana Reyes

    1997-01-01

    Establishes the most outstanding differences between the ISO 9000 norms and total quality management as forms or manners of managing quality used in some information services. Compares two models of total quality: European Foundation far Quality Management and Malcolm Baldrige Awards.

  17. ISO 9000: a guide to world-class quality standards.

    Science.gov (United States)

    Tambolas, S F

    1996-02-01

    World standards for determining companies' commitment to continuous quality improvement (CQI) have been established. These standards are collectively known as ISO-9000. Prudent buyers of goods and services for health care organizations that are also committed to CQI should understand these standards and use them to facilitate supplier certification.

  18. ISO 9000 Quality Systems: Application to Higher Education.

    Science.gov (United States)

    Clery, Roger G.

    This paper describes and explains the 20 elements of the International Organization for Standards 9000 (ISO 9000) series, a model for quality assurance in the business processes of design/development, production, installation and servicing. The standards were designed in 1987 to provide a common denominator for business quality particularly to…

  19. Application of ISO 9000 Standards to Education and Training.

    Science.gov (United States)

    Van den Berghe, Wouter

    1998-01-01

    ISO 9000 certification has the advantages of a measurable framework for quality efforts, continuous improvement, and better customer service. Drawbacks for education and training providers include volume of paperwork, ongoing cost, risk of a growing bureaucracy, and the difficulty of making changes quickly. (SK)

  20. The Challenge of ISO 9000 Certification in Higher Education.

    Science.gov (United States)

    Crawford, Lachlan E. D.; Shutler, Paul M. E.

    1998-01-01

    Describes requirements for ISO 9000 certification in industry, using a factory example. Shows how requirements might be applied in higher education, with "product" being the actual learning outcome and "customer" anyone who purchases educational services. Addresses issues of continuous improvement in higher education. (SK)

  1. Considerations about ISO 14001, and suggestions for the next revision

    DEFF Research Database (Denmark)

    Jørgensen, Tine Herreborg

    2006-01-01

    The aim of this paper is to discuss a number of issues related to ISO 14001:2004, the international standard for environmental management systems (EMS) with the purpose of improving the next edition in order to recognize and reflect new recognitions in approaches to pollution prevention. The stan...

  2. ISO Spectroscopy of H2 in Star Forming Regions

    NARCIS (Netherlands)

    Van den Ancker, M. E.; Wesselius, P. R.; Tielens, A. G. G. M.

    2000-01-01

    Abstract: We have studied molecular hydrogen emission in a sample of 21 YSOs using spectra obtained with the Infrared Space Observatory (ISO). H2 emission was detected in 12 sources and can be explained as arising in either a shock, caused by the interaction of an outflow from an embedded YSO with

  3. Excess Molar Volume Of Binarymixtures From Iso-Propyl Alcohol ...

    African Journals Online (AJOL)

    In this work, binary mixtures from Tertiary-Butyl alcohol (TBA), Toluene (TOL) and Iso-Propyl alcohol (IPA) were prepared at 298K. The mixtures physical properties which includes densities of mixtures (ρ) and pure components (ρI) and mole fractions of components (x) were equally measured at 298K. Excess molar volumes ...

  4. THE STATE OF ISO 9001 CERTIFICATION IN MONTENEGRO

    Directory of Open Access Journals (Sweden)

    Zdravko Krivokapić

    2008-12-01

    Full Text Available In this paper we present the state of ISO 9001 certification in Montenegro. Our research will give results of number of certification comparing with citizen number and Gross Domestic Product (GDP. Furthermore, we will compare them with certain countries in region to estimate approximately number of certifications in the moment of becoming EU member. For the model we choose linear regression.

  5. First results of ISO-SWS observations of Jupiter

    NARCIS (Netherlands)

    Encrenaz, T.; de Graauw, T.; Schaeidt, S.; Lellouch, E.; Feuchtgruber, H.; Beintema, D. A.; Bezard, B.; Drossart, P.; Griffin, M.; Heras, A.; Kessler, M.; Leech, K.; Morris, P.; Roelfsema, P. R.; Roos-Serote, M.; Salama, A.; Vandenbussche, B.; Valentijn, E. A.; Davis, G. R.; Naylor, D. A.

    1996-01-01

    The spectrum of Jupiter has been recorded between 2.75 and 14.5 mu m with the grating mode of the Short-Wavelength Spectrometer (SWS) of ISO. The resolving power is 1500. The main preliminary results of this observation are (1) at 3 mu m, the first spectroscopic signature, probably associated with

  6. An Introduction to Information Security and ISO 27001

    CERN Document Server

    Watkins, Steve G

    2008-01-01

    This new pocket guide will suit both individuals who need an introduction to a topic that they know little about, and also organizations implementing, or considering implementing, some sort of information security management regime, particularly if using ISO/IEC 27001:2005

  7. The ISO-SWS spectrum of planetary nebula NGC 7027

    NARCIS (Netherlands)

    Salas, JB; Pottasch, [No Value; Beintema, DA; Wesselius, PR

    We present the infrared spectrum of the planetary nebula NGC7027 observed with the Short Wavelength Spectrometer (SWS), on board the Infrared Space Observatory (ISO). These data allow us to derive the electron density and, together with the IUE and optical spectra, the electron temperature for

  8. Iso-scalar surfaces, mixing and reaction in turbulent flows

    Science.gov (United States)

    Dopazo, César; Martín, Jesús; Hierro, Juan

    2006-08-01

    Turbulent scalar mixing with chemical reaction is investigated in terms of the local geometrical features of the iso-scalar surfaces—'scalar field topologies'—, using Direct Numerical Simulation data. Two scalars with identical initial distribution, one inert and the other obeying a prescribed Arrhenius-like chemical reaction, evolve in homogeneous isotropic turbulence with a mesh size 256 3. The two local principal curvatures, k and k, of the iso-scalar surface at each point are straightforwardly obtained from the curvature tensor, n, whose elements are the spatial derivatives of the unit vector normal to the iso-surface. The scalar diffusive flux is decomposed into a 'flat-front' contribution plus a curvature induced molecular transport. Expressions for the normal propagating velocity relative to the fluid of iso-surfaces, of both the inert and the reactive scalar fields, are provided making use of the previous decomposition. The 'flat-front' and the curvature induced contributions to the diffusive fluxes, beside to the chemical reaction rate, are correlated with the principal curvatures. Results, including the joint statistics of the principal curvatures and their correlations with the scalar dissipation, are also presented. To cite this article: C. Dopazo et al., C. R. Mecanique 334 (2006).

  9. Observing with the ISO Short-Wavelength Spectrometer

    NARCIS (Netherlands)

    deGraauw, T; Haser, LN; Beintema, DA; Roelfsema, PR; vanAgthoven, H; Barl, L; Bauer, OH; Bekenkamp, HEG; Boonstra, AJ; Boxhoorn, DR; Cote, J; deGroene, P; vanDijkhuizen, C; Evers, J; Feuchtgruber, H; Frericks, M; Genzel, R; Haerendel, G; Heras, AM; vanderHucht, KA; vanderHulst, T; Huygen, R; Jacobs, H; Jakob, G; Kamperman, T; Katterloher, RO; Kester, DJM; Kunze, D; Kussendrager, D; Lahuis, F; Lamers, HJGLM; Leech, K; vanderLei, S; vanderLinden, R; Luinge, W; Lutz, D; Melzner, F; Morris, PW; vanNguyen, D; Ploeger, G; Price, S; Salama, A; Schaeidt, SG; Sijm, N; Smoorenburg, C; Spakman, J; Spoon, H; Steinmayer, M; Stoecker, J; Valentijn, EA; Vandenbussche, B; Visser, H; Waelkens, C; Waters, LBFM; Wensink, J; Wesselius, PR; Wiezorrek, E; Wieprecht, E; Wijnbergen, JJ; Wildeman, KJ; Young, E

    1996-01-01

    The Short-Wavelength Spectrometer (SWS) is one of the four instruments on-board ESA's Infrared Space Observatory (ISO), launched on November 17, 1995. The spectrometer covers the wavelength range of 2.38 to 4.2 mu m with a spectral resolution ranging from 1000 to 2000. By inserting Fabry-Perot

  10. The hidden networks of knowledge in ISO 14001

    DEFF Research Database (Denmark)

    Jørgensen, Ulrik

    2003-01-01

    The ISO 14001 standard is defined as a management standard based on an assumed existing knowledge base of what is to be considered being environmental problems. In the implementation of the standard is become evident, that its use and impact is entirely dependent of the existence of networks...

  11. The soul of the machine: continual improvement in ISO 14001

    NARCIS (Netherlands)

    Brouwer, M.A.C.; van Koppen, C.S.A.|info:eu-repo/dai/nl/166978841

    2008-01-01

    Continual improvement is a key component of ISO 14001, but in contrast to most other specifications in the standard, it is difficult to assess. Based on interviews with 19 certified companies in the chemical, food, and environmental services sectors, this article explores the dynamics of continual

  12. The soul of the machine: continual improvement in ISO 14001.

    NARCIS (Netherlands)

    Brouwer, M.A.C.; Koppen, van C.S.A.

    2008-01-01

    Continual improvement is a key component of ISO 14001, but in contrast to most other specifications in the standard, it is difficult to assess. Based on interviews with 19 certified companies in the chemical, food, and environmental services sectors, this article explores the dynamics of continual

  13. Covenants with Weak Swords: ISO 14001 and Facilities' Environmental Performance

    Science.gov (United States)

    Potoski, Matthew; Prakash, Aseem

    2005-01-01

    Voluntary environmental programs are codes of progressive environmental conduct that firms pledge to adopt. This paper investigates whether ISO 14001, a voluntary program with a weak sword--a weak monitoring and sanctioning mechanism--can mitigate shirking and improve participants' environmental performance. Sponsored by the International…

  14. ISO observations of Titan with SWS/grating

    Science.gov (United States)

    Coustenis, A.; Encrenaz, T.; Salama, A.; Lellouch, E.; Gautier, D.; Kessler, M. F.; deGraauw, T.; Samuelson, R. E.; Bjoraker, G.; Orton, G.

    1997-01-01

    The observations of Titan performed by the Infrared Space Observatory (ISO) short wavelength spectrometer (SWS), in the 2 micrometer to 45 micrometer region using the grating mode, are reported on. Special attention is given to data from Titan concerning 7 micrometer to 45 micrometer spectral resolution. Future work for improving Titan's spectra investigation is suggested.

  15. Information Asymmetries as Trade Barriers: ISO 9000 Increases International Commerce

    Science.gov (United States)

    Potoski, Matthew; Prakash, Aseem

    2009-01-01

    Spatial, cultural, and linguistic barriers create information asymmetries between buyers and sellers that impede international trade. The International Organization for Standardization's ISO 9000 program is designed to reduce these information asymmetries by providing assurance about the product quality of firms that receive its certification.…

  16. [Effects of the ISO 15189 accreditation on Nagoya University Hospital].

    Science.gov (United States)

    Yoshiko, Kenichi

    2012-07-01

    The Department of Clinical Laboratory, Nagoya University Hospital acquired ISO 15189 accreditation in November, 2009. The operation of our Quality Management System (QMS) was first surveyed in October, 2010. In this paper, we reported the activity for the preparation and operation of our QMS and the effects of ISO 15189 accreditation. We investigated the changes in the number and content on nonconformities, incident reports and complaints before and after accreditation as indicators to evaluate the effect of ISO 15189 accreditation. Post accreditation, the number of nonconformities and incident reports decreased, seeming to show an improvement of quality of the laboratory activity; however, the number of complaints increased. We identified the increase of complaints at the phlebotomy station. There had been some problems with blood sampling in the past, but it seemed that staff had a high level of concern regarding these problems at the phlebotomy station and took appropriate measures to resolve the complaints. We confirmed that the ISO 15189 accreditation was instrumental in the improvements of the safety and efficiency on laboratory works. However there was a problem that increase of overtime works to operate the QMS. We deal with development of a laboratory management system using IT recourses to solve the problem.

  17. Significance of ISO 9000 Quality Management System for ...

    African Journals Online (AJOL)

    The objective of this paper is to analyze the significances of ISO 9000 Quality Management System implementation for performance improvement. Two consecutive cases are studied cascaded one over the other. Harar Brewery Share Company (HBSC), one of the Ethiopian Quality Award winners in 2009 is taken as the first ...

  18. The NF1 gene contains hotspots for L1 endonuclease-dependent de novo insertion.

    Directory of Open Access Journals (Sweden)

    Katharina Wimmer

    2011-11-01

    Full Text Available Long interspersed (L1 and Alu elements are actively amplified in the human genome through retrotransposition of their RNA intermediates by the -100 still retrotranspositionally fully competent L1 elements. Retrotransposition can cause inherited disease if such an element is inserted near or within a functional gene. Using direct cDNA sequencing as the primary assay for comprehensive NF1 mutation analysis, we uncovered in 18 unrelated index patients splicing alterations not readily explained at the genomic level by an underlying point-mutation or deletion. Improved PCR protocols avoiding allelic drop-out of the mutant alleles uncovered insertions of fourteen Alu elements, three L1 elements, and one poly(T stretch to cause these splicing defects. Taken together, the 18 pathogenic L1 endonuclease-mediated de novo insertions represent the largest number of this type of mutations characterized in a single human gene. Our findings show that retrotransposon insertions account for as many as -0.4% of all NF1 mutations. Since altered splicing was the main effect of the inserted elements, the current finding was facilitated by the use of RNA-based mutation analysis protocols, resulting in improved detection compared to gDNA-based approaches. Six different insertions clustered in a relatively small 1.5-kb region (NF1 exons 21(16-23(18 within the 280-kb NF1 gene. Furthermore, three different specific integration sites, one of them located in this cluster region, were each used twice, i.e. NM_000267.3(NF1:c.1642-1_1642 in intron 14(10c, NM_000267.3(NF1:c.2835_2836 in exon 21(16, and NM_000267.3(NF1:c.4319_4320 in exon 33(25. Identification of three loci that each served twice as integration site for independent retrotransposition events as well as 1.5-kb cluster region harboring six independent insertions supports the notion of non-random insertion of retrotransposons in the human genome. Currently, little is known about which features make sites

  19. Genistein improves schistosomiasis liver granuloma and fibrosis via dampening NF-kB signaling in mice.

    Science.gov (United States)

    Wan, Chunpeng; Jin, Fen; Du, Youqin; Yang, Kang; Yao, Liangliang; Mei, Zhigang; Huang, Weifeng

    2017-04-01

    In schistosomiasis, egg deposition in the liver contributes to the formation of hepatic granuloma and fibrosis, which are the most serious clinical pathological features. It has been proposed that activation of the nuclear factor kappa B (NF-κB) signaling pathways is closely associated with the development of hepatic granuloma and fibrosis. Genistein has been shown to inhibit the activity of NF-κB signaling pathways, which might be a potential agent to protect against Schistosoma japonicum egg-induced liver granuloma and fibrosis. In this study, liver granuloma and fibrosis were induced by infecting BALB/c mice with 18 ± 3 cercariae of S. japonicum. At the beginning of egg granuloma formation (early phase genistein treatment from 4 to 6 weeks after infection) or after the formation of liver fibrosis (late phase genistein treatment from 6 to 10 weeks after infection), the infected mice were injected with genistein (25, 50 mg/kg). The results revealed that genistein treatment significantly decreased the extent of hepatic granuloma and fibrosis in infected mice. The activity of NF-κB signaling declined sharply after the treatment with genistein, as evidenced by the inhibition of NF-κB-p65, phospho-NF-κB-p65, and phospo-IκB-α expressions, as well as the expression of IκB-α and the messenger RNA (mRNA) expression of inflammatory cytokines (MCP1, TNFα, IL1β, IL4, IL10) mediated by NF-κB signaling pathways in the early phase of the infection. Moreover, western blot and immunohistochemistry assays demonstrated that the contents of α-smooth muscle actin (α-SMA) and transforming growth factor-β were dramatically reduced in liver tissue under the treatment of genistein in the late phase of the infection. At the same time, the mRNA expression of MCP1, TNFα, and IL10 was inhibited markedly. These results provided evidence that genistein reduces S. japonicum egg-induced liver granuloma and fibrosis, at least partly due to decreased NF-κB signaling, and

  20. Launching the chaotic realm of iso-fractals: A short remark

    Energy Technology Data Exchange (ETDEWEB)

    O' Schmidt, Nathan [Department of Mathematics, Boise State University, 1910 University Drive, Boise, ID 83725 (United States); Katebi, Reza [Department of Physics, California State University in Fullerton, 800 North State College Boulevard, Fullerton, CA 92831 (United States); Corda, Christian [Institute for Theoretical Physics and Advanced Mathematics Einstein-Galilei (IFM), Via Santa Gonda 14, 59100 Prato (Italy)

    2015-03-10

    In this brief note, we introduce the new, emerging sub-discipline of iso-fractals by highlighting and discussing the preliminary results of recent works. First, we note the abundance of fractal, chaotic, non-linear, and self-similar structures in nature while emphasizing the importance of studying such systems because fractal geometry is the language of chaos. Second, we outline the iso-fractal generalization of the Mandelbrot set to exemplify the newly generated Mandelbrot iso-sets. Third, we present the cutting-edge notion of dynamic iso-spaces and explain how a mathematical space can be iso-topically lifted with iso-unit functions that (continuously or discretely) change; in the discrete case examples, we mention that iteratively generated sequences like Fibonacci’s numbers and (the complex moduli of) Mandelbrot’s numbers can supply a deterministic chain of iso-units to construct an ordered series of (magnified and/or de-magnified) iso-spaces that are locally iso-morphic. Fourth, we consider the initiation of iso-fractals with Inopin’s holographic ring (IHR) topology and fractional statistics for 2D and 3D iso-spaces. In total, the reviewed iso-fractal results are a significant improvement over traditional fractals because the application of Santilli’s iso-mathematics arms us an extra degree of freedom for attacking problems in chaos. Finally, we conclude by proposing some questions and ideas for future research work.

  1. 企業経営とISO14000

    OpenAIRE

    佐々木,浩幸

    1999-01-01

    今日の日本の企業のほとんどは,国際化の中での経営活動を展開している。このため,国際標準化機構(ISO)の定めた国際規格を取得することが,国際的な経営活動をする企業として生き残るために不可欠な要素となった。今日ISOのうちでも注目されているのは,9000と14000である。ISO9000は品質システムに関する国際規格で,ISO14000は,環境マネジメントシステムに関する国際規格である。本論文では,全人類の課題である環境問題の重要性の視点から,このISO14000と企業経営について論じた。まず,ISO14000取得のメリットデメリットについて論じ,世界及び日本におけるISO14000の取得状況について述べた。特に日本の状況については,都道府県別にその特徴の分析を,基本的データを分かりやすく加工して,行った。そして,日本の企業の将来のためには,ISO14000取得は必須条件であり,取得のための問題点,その解決策,将来展望について論じた。...

  2. Evaluation of Virological Microparticle Enzyme Immunoassay According to the ISO 15189: Real-Life Experience in a University Hospital.

    Science.gov (United States)

    Lafontaine, Sophie; Prin-Mathieu, Christine; Velay, Aurélie; Agulles, Odette; Schvoerer, Evelyne; Jeulin, Hélène

    2016-01-01

    The International Standard ISO 15189 based on the ISO 9001:2008 emphasizes specific requirements for quality and ability of medical laboratories. The accreditation of medical laboratories according to ISO 15189 includes the validation of biological methods, which depends on collection of bibliographic data and experimental proofs. Moreover, these results must be compared to provider data sheets and independent scientific data. In the immunodiagnostic field, independent published data are deeply lacking. The aim of our work was to share experience of method validation for virological immune markers on the widely used Architect i2000sr. After risk analysis, intra- and inter-assay variability, and inter-sample contamination were evaluated for each method, and sensitivity was investigated for antigen detection tests. A comparison between the two Architect i2000sr available in our laboratory was also performed. All tested methods were consistent with the manufacturer data (from the data sheet). No inter-sample contamination was observed. Both devices are broadly equivalent and can be used indifferently or as a backup solution of the other. To our knowledge, those results are the first independent complete data on the reliability of the Architect i2000sr in real-life experience. These data are needed to the accreditation of our platform and potentially useful for the accreditation of other laboratories using the same equipment.

  3. Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.

    Science.gov (United States)

    Kim, Jinhee; Kang, Won-Hee; Hwang, Jeena; Yang, Hee-Bum; Dosun, Kim; Oh, Chang-Sik; Kang, Byoung-Cheorl

    2014-08-01

    The protein-protein interaction between VPg (viral protein genome-linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad-spectrum resistance in Chinese cabbage (Brassica rapa) to Turnip mosaic virus (TuMV), which is one of the most important potyviruses, by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and B. rapa eIF(iso)4E. The seven amino acids in the cap-binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap-binding pockets, and mutated. Yeast two-hybrid assay and co-immunoprecipitation analysis demonstrated that W95L, K150L and W95L/K150E amino acid mutations of B. rapa eIF(iso)4E interrupted its interaction with TuMV VPg. All eIF(iso)4E mutants were able to complement an eIF4E-knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L, W95L/K150E and eIF(iso)4E wild-type were over-expressed in a susceptible Chinese cabbage cultivar. Evaluation of the TuMV resistance of T1 and T2 transformants demonstrated that the over-expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge-based approaches for the engineering of broad-spectrum resistance in Chinese cabbage. © 2014 BSPP AND JOHN WILEY & SONS LTD.

  4. Reasons to Adopt ISO 50001 Energy Management System

    Directory of Open Access Journals (Sweden)

    Frederic Marimon

    2017-09-01

    Full Text Available Purpose: The main aim of this paper is to analyze the relationships between the corporate motivations that lead organizations to establish the ISO 50001 Energy Management System (EnMS standard, and the difficulties and benefits derived from its adoption. Design/methodology/approach: Three independent exploratory factor analyses (EFA were conducted in order to identify (i sources of motivation: social requirements, ecology drivers, and competitive advantage; (ii the difficulties of an ISO 50001 adoption: operational difficulties and organizational difficulty; and (iii types of benefits: ecological benefits and operational benefits. In a second step, an exploratory path analysis, performed through Structural Equation Modeling (SEM, was used to analyze the relations among motivation, difficulties, and benefits related to the adoption of the ISO 50001 standard. Findings: Social requirements explain operational difficulties, which in turn impacts on operational benefits. Ecology drivers are directly related to ecological benefits. Organizational difficulties have an inverse relationship with operational and ecological benefits. Operational difficulties are related to operational benefits and ecological benefits. Research limitations/implications: The questionnaire was disseminated to 87 Spanish companies with ISO 50001 certification. Managers and other practitioners such as consultants, auditing companies, and official organizations in charge of developing standards might find useful implications. Originality/value: The standard was published in 2011 by the International Organization for Standardization (ISO. This paper contributes to assessment of the benefits of the standard by collecting information directly from the pioneer organizations that have adopted it, and provides clues on how to implement the standard and improve it in future.

  5. ISO 15189 Accreditation: Navigation Between Quality Management and Patient Safety

    Directory of Open Access Journals (Sweden)

    Plebani Mario

    2017-09-01

    Full Text Available Accreditation is a valuable resource for clinical laboratories and the development of an International Standard for their accreditation represented a milestone on the path towards improved quality and safety in laboratory medicine. The recent revision of the International Standard, ISO 15189, has further strengthened its value not only for improving the quality system of a clinical laboratory but also for better answering the request for competence, focus on customers’ needs and ultimate value of laboratory services. Although in some countries more general standards such as ISO 9001 for quality systems or ISO 17025 for testing laboratories are still used, there is increasing recognition of the value of ISO 15189 as the most appropriate and useful standard for the accreditation of medical laboratories. In fact, only this International Standard recognizes the importance of all steps of the total testing process, namely extra-analytical phases, the need to focus on technical competence in addition to quality systems, and the focus on customers’ needs. However, the number of accredited laboratories largely varies between European countries and also major differences affect the approaches to accreditation promoted by the national bodies. In particular, some national accreditation bodies perpetuate the use of fixed scopes, while the European co-operation for accreditation (EA and the European Federation of Laboratory Medicine (EFLM Working Group promote the use of flexible scopes. Major issues in clinical laboratory accreditation are the verification of examination procedures for imprecision, trueness and diagnostic accuracy and for estimating measurement uncertainty. In addition, quality indicators (QIs are a fundamental requirement of the ISO 15189 International Standard.

  6. Barriers to ISO 9001 implementation in Moroccan organizations: Empirical study

    Directory of Open Access Journals (Sweden)

    Noussaiba Bounabri

    2018-02-01

    Full Text Available Purpose: The purpose of this paper is to explore obstacles to ISO 9001 quality management system implementation in Moroccan firms. Design/methodology/approach: A questionnaire survey has been conducted among a heterogeneous sample of 115 organizations, operating in different sectors in Morocco. The authors have studied barriers to quality initiatives in general and obstacles to QMS implementation in particular through an extensive literature review. Questions related to profiles of respondents, reasons of seeking certification, external consultancy and barriers to ISO 9001 implementation experienced by surveyed organizations. For the purpose of this study, authors considered three categories of quality inhibiting factors: organizational, technical and costs related barriers. Findings: Results indicate that surveyed companies sought ISO 9001 certification mainly for marketing reasons and experienced many difficulties during the implementation process. Barriers reported by respondents were mostly organizational. Resistance to change headed the list according to participants’ opinion. Also, findings highlighted the prominence of bureaucracy and poor interdependence between departments in organizations. Lack of communication, poor top management commitment and insufficient trainings were also ascertained to be obstacles to QMS implementation in Morocco. Originality/value: Earlier studies were led by different researchers in different countries about barriers to quality initiatives in general and to ISO 9001 implementation in particular. Few of those studies were conducted in Arab speaking countries but no research has been carried in Morocco. This study on obstacles to QMS implementation in Morocco will help in completing the jigsaw of difficulties faced by organizations worldwide when preparing to ISO 9001 certification.

  7. EBV induces persistent NF-κB activation and contributes to survival of EBV-positive neoplastic T- or NK-cells.

    Directory of Open Access Journals (Sweden)

    Honami Takada

    Full Text Available Epstein-Barr virus (EBV has been detected in several T- and NK-cell neoplasms such as extranodal NK/T-cell lymphoma nasal type, aggressive NK-cell leukemia, EBV-positive peripheral T-cell lymphoma, systemic EBV-positive T-cell lymphoma of childhood, and chronic active EBV infection (CAEBV. However, how this virus contributes to lymphomagenesis in T or NK cells remains largely unknown. Here, we examined NF-κB activation in EBV-positive T or NK cell lines, SNT8, SNT15, SNT16, SNK6, and primary EBV-positive and clonally proliferating T/NK cells obtained from the peripheral blood of patients with CAEBV. Western blotting, electrophoretic mobility shift assays, and immunofluorescent staining revealed persistent NF-κB activation in EBV-infected cell lines and primary cells from patients. Furthermore, we investigated the role of EBV in infected T cells. We performed an in vitro infection assay using MOLT4 cells infected with EBV. The infection directly induced NF-κB activation, promoted survival, and inhibited etoposide-induced apoptosis in MOLT4 cells. The luciferase assay suggested that LMP1 mediated NF-κB activation in MOLT4 cells. IMD-0354, a specific inhibitor of NF-κB that suppresses NF-κB activation in cell lines, inhibited cell survival and induced apoptosis. These results indicate that EBV induces NF-κB-mediated survival signals in T and NK cells, and therefore, may contribute to the lymphomagenesis of these cells.

  8. EBV induces persistent NF-κB activation and contributes to survival of EBV-positive neoplastic T- or NK-cells.

    Science.gov (United States)

    Takada, Honami; Imadome, Ken-Ichi; Shibayama, Haruna; Yoshimori, Mayumi; Wang, Ludan; Saitoh, Yasunori; Uota, Shin; Yamaoka, Shoji; Koyama, Takatoshi; Shimizu, Norio; Yamamoto, Kouhei; Fujiwara, Shigeyoshi; Miura, Osamu; Arai, Ayako

    2017-01-01

    Epstein-Barr virus (EBV) has been detected in several T- and NK-cell neoplasms such as extranodal NK/T-cell lymphoma nasal type, aggressive NK-cell leukemia, EBV-positive peripheral T-cell lymphoma, systemic EBV-positive T-cell lymphoma of childhood, and chronic active EBV infection (CAEBV). However, how this virus contributes to lymphomagenesis in T or NK cells remains largely unknown. Here, we examined NF-κB activation in EBV-positive T or NK cell lines, SNT8, SNT15, SNT16, SNK6, and primary EBV-positive and clonally proliferating T/NK cells obtained from the peripheral blood of patients with CAEBV. Western blotting, electrophoretic mobility shift assays, and immunofluorescent staining revealed persistent NF-κB activation in EBV-infected cell lines and primary cells from patients. Furthermore, we investigated the role of EBV in infected T cells. We performed an in vitro infection assay using MOLT4 cells infected with EBV. The infection directly induced NF-κB activation, promoted survival, and inhibited etoposide-induced apoptosis in MOLT4 cells. The luciferase assay suggested that LMP1 mediated NF-κB activation in MOLT4 cells. IMD-0354, a specific inhibitor of NF-κB that suppresses NF-κB activation in cell lines, inhibited cell survival and induced apoptosis. These results indicate that EBV induces NF-κB-mediated survival signals in T and NK cells, and therefore, may contribute to the lymphomagenesis of these cells.

  9. [Efforts to achieve and effects of acquiring ISO 15189 in Tokushima University Hospital].

    Science.gov (United States)

    Shono, Kazuko; Kishi, Misako; Satou, Mituyo; Nagamine, Yasunori; Doi, Tosio

    2009-12-01

    The medical laboratory of Tokushima University Hospital acquired ISO 15189, an international standard for medical laboratories, on July 6th, 2007, resulting in it achieving the 24th place in Japan and 5th place among national university hospitals. The first surveillance was just performed on October 6th, 2008. Tokushima University Hospital, in which our medical laboratory is included as one section, already succeeded in acquiring ISO 9001, PrivacyMark System, and Quality Health Care ver. 5 before accomplishing ISO 15189. To achieve ISO 15189, we prepared documents based on ISO 9001 without any consultation, resulting in a review of the difference between ISO 9001 and ISO 15189 after the preliminary survey. Although achieving ISO 15189 resulted in an improvement in the reliability of laboratory results and accuracy, leading to the development of our technical skills and awareness, and sharing of knowledge, we consider that the considerable investment of time to prepare the requirements remains to be overcome.

  10. Analysis of the Air Force ISO 14001 Pilot Study Conducted by DoD

    National Research Council Canada - National Science Library

    Harris, Rodney

    2000-01-01

    The Department of Defense (DoD) conducted an ISO 14001 pilot study with the primary goal of determining how ISO 14001 could help DoD organizations reduce risks, improve compliance with environmental regulations, enhance stewardship...

  11. Implementace normy ISO 14001 v průmyslovém podniku

    OpenAIRE

    HOUZIM, Pavel

    2013-01-01

    The work deals with the implementation of an environmental management system according to ISO 14001 in a particular enterprise. The aim was to design an appropriate way of implementation of ISO 14001 certifications topped successful.

  12. Impacts of the ISO 14000 management system on sustainable forest management in South Africa

    CSIR Research Space (South Africa)

    Von Maltitz, Graham P

    2000-01-01

    Full Text Available The implementation of the International Organization for Standards (ISO) series of environmental standards (ISO 14000) within the South African forestry industry will, over time, result in a forestry management standard equal to, or superior to...

  13. TagParser: well on the way to ISO-TC37 conformance

    OpenAIRE

    Francopoulo, Gil

    2007-01-01

    We present rapidly the family of standards that are currently under development within ISO-TC37. Then as an example of application of these ISO specifications for French, a concrete industrial parser is de-scribed: TagParser.

  14. Cosmic water traced by Europe's space telescope ISO

    Science.gov (United States)

    1996-05-01

    In retracing this history, ISO also observes water in the form of ice in cooler regions around the stars, and in the dust surrounding young stars, from which planets could evolve. Comets represent an intermediate stage in planet-building, and they contain much water ice. According to one hypothesis the newly formed Earth received some of its water directly from impacting comets. Water vapour in the Earth's atmosphere has prevented telescopes on the ground from detecting the water vapour among the stars, except in very unusual circumstances. ISO orbiting in space escapes the impediment of the atmosphere. Excellent onboard instruments register the characteristic infrared signatures of water vapour, water ice and many other materials. When ISO scrutinizes selected objects, it detects emissions or absorptions of infrared rays at particular wavelengths, or "lines" in a spectrum, which reveal the presence of identifiable atoms, molecules and solids. The Short Wavelength Specrometer and the Long Wavelength Spectrometer provide detailed chemical diagnoses, and the photometer ISOPHOT and camera ISOCAM also have important spectroscopic capabilities. Examples of water detection were among many topics reviewed at the First ISO Science Workshop held at ESA's Research and Technology Centre (ESTEC) in Noordwijk, the Netherlands (29-31 May) when 300 astronomers from Europe, the USA and Japan gathered to assess results from ISO since its launch on 17 November 1995. The Long Wavelength Spectrometer has made remarkable observations of water-vapour lines in the vicinity of dying stars and in star-forming regions. So has the Short Wavelength Spectrometer, which also detects water ice. The photometer lSOPHOT has registered water ice in a large number of objects. Although fascinated by the natural history of water in the cosmos, astronomers have more technical reasons for welcoming ISO's observations. They can use thc details in a spectrum to reduce the abundance of water and its

  15. NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqr activation in echiuran worm Urechis unicinctus

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xiaolong; Qin, Zhenkui; Li, Xueyu; Ma, Xiaoyu; Gao, Beibei; Zhang, Zhifeng, E-mail: zzfp107@ouc.edu.cn

    2016-06-15

    Highlights: • Sulfide activates sqr transcription against respiratory toxicity in Urechis unicinctus. • Sulfide increases expressions and activities of NF1, Sp1 and HSF1 in a time-dependent manner. • NF1 and Sp1 participate in both basal and early sulfide-induced sqr transcription. • HSF1 functions more significantly than NF1 and Sp1 in sulfide-induced sqr transcription. • Transcription factors NF1, Sp1 and HSF1 enhance sqr promoter activity synergistically. - Abstract: Background: Sulfide is a well-known environmental toxic substance. Mitochondrial sulfide oxidation is a main mechanism of sulfide detoxification in organisms, and sulfide: quinone oxidoreductase (SQR) is a key enzyme which is involved in transferring electrons from sulfide to ubiquinone and converting sulfide into thiosulfate. Previous studies have revealed the SQR-mediated mitochondrial sulfide oxidation exists in the echiuran worm Urechis unicinctus, and its sqr mRNA level increased significantly when the worm is exposed to sulfide. In this study, we attempt to reveal the synergistic regulation of transcription factors on sulfide-induced sqr transcription in U. unicinctus. Methods: ChIP and EMSA were used to identify the interactions between sqr proximal promoter (from −391 to +194 bp) and transcription factors NF1 (nuclear factor 1) and Sp1 (specificity protein 1). Site-directed mutation and transfection assays further revealed their binding sites and synergistic roles of HSF1, NF1 and Sp1 in the sqr transcription. When U. unicinctus were exposed to 150 μM sulfide, the expression levels and nuclear contents of NF1 and Sp1 were examined by Western blotting, and the binding contents between NF1 or Sp1 and the sqr promoter were also detected by ChIP. Results: Transcription factors NF1 and Sp1 were confirmed to interact with the sqr proximal promoter, and their binding sites were identified in −75 to −69 bp for NF1 and −210 to −201 bp for Sp1. Transfection assays showed mutation

  16. On the composition of ISO 25964 hierarchical relations (BTG, BTP, BTI)

    NARCIS (Netherlands)

    Alexiev, A; Isaac, A.H.J.C.A.; Lindenthal, J

    2015-01-01

    Knowledge organization systems (KOS) can use different types of hierarchical relations: broader generic (BTG), broader partitive (BTP), and broader instantial (BTI). The latest ISO standard on thesauri (ISO 25964) has formalized these relations in a corresponding OWL ontology (De Smedt et al., ISO

  17. ISO sertifikaat kuulutab tootja mainet üle maailma / Ain Alvela

    Index Scriptorium Estoniae

    Alvela, Ain, 1967-

    2006-01-01

    Ilmunud ka: Delovõje Vedomosti 12. apr. lk. 24-25. ISO standardi rakendamine tagab toodangu kvaliteedi ja annab firmale usaldusväärsuse. Kommenteerib Ain Noormägi. Vt. samas: ISO 9001; ISO 14 001; Universaalne standard kohandub ettevõtte vajadustega

  18. Juhtimissüsteemide selgroog. Lean ning ISO - terviklahendused tootmise haldamiseks / Anton Ovsjankin

    Index Scriptorium Estoniae

    Ovsjankin, Anton

    2011-01-01

    Kasvanud on juurutatavate Lean ja ISO süsteemide arv, kuid on kasvanud ka nende juurutamise ja arendamisega seotud ebaõnnestumiste arv. Põhjustest, miks jäävad paljud Leani ja ISO juurutanud ettevõtted ilma lubatud tulemusteta. Leani ja ISO haldamiseks vajalikest teguritest

  19. Antioxidant mechanisms of iso-6-cassine in suppressing seizures induced by pilocarpine

    Directory of Open Access Journals (Sweden)

    Rivelilson Mendes de Freitas

    2011-06-01

    Full Text Available The aim of this study was to evaluate the in vitro antioxidant effects of 12-[(2R,5R,6R-5-hydroxy-6-methylpiperidin-2-yl]dodecan-2-one (iso-6-cassine; ISO and the anticonvulsant effects of ISO on pilocarpine-induced seizures in rats. Wistar rats were treated with 0.9% saline (i.p., control group, pilocarpine (400 mg/kg, i.p., pilocarpine group, and the association of ISO (1.0 mg/kg, i.p. plus pilocarpine (400 mg/kg, i.p., 30 min after administration of ISO (ISO plus pilocarpine group. After the treatments all groups were observed for 1h. The antioxidant effect of ISO on the pilocarpine model was assessed by determining the activity of glutathione peroxidase (GPx, glutathione-S-transferase (GST and catalase (CAT as well as the levels of reactive species (RS and lipid peroxidation (LP. In vitro, ISO (5 μM reduced RS and LP. ISO (1.0 mg/kg and abolished seizures and death induced by pilocarpine in rats. ISO protected against the increase in the RS and LP levels, GST activity as well as the inhibition of GPx activity caused by pilocarpine. In addition, ISO increased the catalase activity in hippocampus of seized rats. In conclusion, the dta suggest that ISO can present anticonvulsant and antioxidant properties in the pilocarpine model of seizures in rats.

  20. 76 FR 4104 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2011-01-24

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings January 13... Commission staff may attend the following Midwest ISO-related meetings during the 2011 year: Advisory.... Paul, MN) September 14 October 19 November 16 December 6 Midwest ISO Informational Forum (3 p.m.-5 p.m...

  1. 75 FR 51996 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2010-08-24

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings August 16... Commission staff may attend the following Midwest ISO-related meetings: Advisory Committee (10 a.m.-3 p.m.... Paul Hotel, 350 Market St., St. Paul, MN) September 15 October 20 November 17 December 1 Midwest ISO...

  2. 75 FR 5779 - Notice Providing Agenda for Technical Conference on RTO/ISO Responsiveness

    Science.gov (United States)

    2010-02-04

    ... Energy Regulatory Commission Notice Providing Agenda for Technical Conference on RTO/ISO Responsiveness.... ER09-1050-000, ER09- 1192-000. ISO New England, Inc. and New England Docket No. ER09-1051-000. Power... , (202) 502-8048. Kimberly D. Bose, Secretary. Agenda--Technical Conference on RTO/ISO Responsiveness...

  3. 75 FR 3228 - Notice of Commission Staff Attendance at Midwest ISO Meetings

    Science.gov (United States)

    2010-01-20

    ... Energy Regulatory Commission Notice of Commission Staff Attendance at Midwest ISO Meetings January 12... Commission staff may attend the following Midwest ISO-related meetings: Advisory Committee (10 a.m.-3 p.m... October 20 November 17 December 1 Midwest ISO Informational Forum (3 p.m.-5 p.m., ET) January 19 February...

  4. 78 FR 38027 - ISO New England Inc.; Notice of Initiation of Proceeding and Refund Effective Date

    Science.gov (United States)

    2013-06-25

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL13-72-000] ISO New England... III.A.15 of Appendix A of ISO New England Inc.'s existing tariff. Dominion Energy Marketing, Inc. and ISO New England Inc., 143 FERC ] 61,233 (2013). The refund effective date in Docket No. EL13-72-000...

  5. 75 FR 16096 - New England Power Generators Association Inc., Complainant v. ISO New England Inc., Respondent...

    Science.gov (United States)

    2010-03-31

    ... Energy Regulatory Commission New England Power Generators Association Inc., Complainant v. ISO New England Inc., Respondent; ISO New England Inc. and New England Power Pool; Notice of Complaint March 24... Generators Association Inc. (Complainant) filed a formal complaint against ISO New England Inc. (Respondent...

  6. Human stem cell modeling in neurofibromatosis type 1 (NF1).

    Science.gov (United States)

    Wegscheid, Michelle L; Anastasaki, Corina; Gutmann, David H

    2017-04-06

    The future of precision medicine is heavily reliant on the use of human tissues to identify the key determinants that account for differences between individuals with the same disorder. This need is exemplified by the neurofibromatosis type 1 (NF1) neurogenetic condition. As such, individuals with NF1 are born with a germline mutation in the NF1 gene, but may develop numerous distinct neurological problems, ranging from autism and attention deficit to brain and peripheral nerve sheath tumors. Coupled with accurate preclinical mouse models, the availability of NF1 patient-derived induced pluripotent stem cells (iPSCs) provides new opportunities to define the critical factors that underlie NF1-associated nervous system disease pathogenesis and progression. In this review, we discuss the generation and potential applications of iPSC technology to the study of NF1. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Targeting Signaling to YAP for the Therapy of NF2

    Science.gov (United States)

    2015-10-01

    VIII cranial nerve, but also meningiomas and ependymomas. The only effective current therapy for NF2 involves repeated and debilitating surgeries...clear mechanism of Merlin’s tumor suppressor function . Our studies have shown that inactivation of Merlin/NF2 de-regulates the E3 ubiquitin ligase...indicated that this reported functions as anticipated in response to overexpressed YAP. We therefore generated several reporter-expressing NF2 mutant cell

  8. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.

    Science.gov (United States)

    Harris, J; Ayyub, C; Shaw, G

    1991-09-01

    We have initiated a multidisciplinary project that aims to dissect and ultimately define the functions of the long and unusual C-terminal "tail" sequences of the two high molecular weight neurofilament subunits, NF-M and NF-H. A series of recombinant fusion proteins containing selected NF-M and NF-H tail sequences were constructed using appropriate cDNAs. These fusion proteins were used to further define the epitopes for a variety of widely used neurofilament antibodies, including NN18 and N52, which are now available commercially from several companies. We also measured the SDS-PAGE mobility of the fusion proteins and found that, like the native neurofilament tails, the fusion proteins ran considerably slower than predicted from their molecular weight. Since all fusion proteins produced so far exhibit this characteristic we conclude that all segments of the NF-M and NF-H tail share this unusual property. Finally we were able to produce novel and potentially useful polyclonal and monoclonal antibodies to selected segments of NF-M and NF-H sequence. These antibody studies showed that the extreme C-termini of NF-M and NF-H are immunologically absolutely distinct from one another and also indicate that the extreme C-terminus of NF-M is immunologically much more conserved than the analogous region of NF-H. These findings are in complete agreement with our conclusions derived from amino acid sequence analysis, and further underline the possible functional importance of the extreme C-terminus of NF-M. We also show that the unusual immunological properties of the bovine NF-M tail we have previously observed do not extend to the extreme C-terminal region, which appears immunologically no different from the analogous region of other NF-M molecules. The peculiarities of bovine NF-M could be explained by the presence of a KSP motif that resembles the NF-H KSP prototype.

  9. The New Food Safety Standard ISO 22000. Assessment, Comparison and Correlation with HACCP and ISO 9000:2000. The Practical Implementation in Victual Business

    OpenAIRE

    Mamalis, Spyridon; Kafetzopoulos, Dimitrios P.; Aggelopoulos, Stamatis

    2009-01-01

    The present paper aims to study the application of new standard of food safety: ISO 22000:2005 in the catering industry. At the beginning the study made a comparison between ISO 22000 with the HACCP system and the ISO 9000:2000 standard, in order to identify their similarities and differences, while it pinpoints and records the reasons for creating and applying the ISO 22000 standard. Due to the preliminary nature of this study, we are given the opportunity to assess the practical application...

  10. Recent operational experiences of FILMTEC NF270 membrane in Europe.

    Science.gov (United States)

    Majamaa, Katariina; Warczok, Justyna; Lehtinen, Martti

    2011-01-01

    Nanofiltration (NF) is an attractive technology for potable and industrial water treatment because NF operates between ultrafiltration (UF) and reverse osmosis (RO) membranes. NF is designed to remove a high percentage of organic contaminants (humic acids, pesticides, color bodies) while passing a medium to high percentage of salt. Compared to UF membranes, the NF product water quality is significantly better; compared to comparable RO treatment systems NF systems require lower operating pressures. Due to these features, NF is increasingly used in a broad range of water treatment applications. The general applications include softening, as well as color, organics and micro-organism removal. DOW FILMTEC NF270-400 is one the most frequently used elements in water treatment and this paper presents examples of three recent NF270-400 installations in Europe. The first two plants, Eupen and Stembert, are located in Belgium and produce potable water from surface water. The third one is a Scandinavian plant which purifies groundwater for a brewery and soft drink production. The presented operation results prove NF to be a highly competitive technique for low cost water treatment.

  11. Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.

    Directory of Open Access Journals (Sweden)

    Bin Yi

    Full Text Available AIMS: To investigate if nuclear NF-κB p65 expression in ex vivo isolated peripheral blood mononuclear cells correlates with urinary MCP-1 or RANTES and the severity of type 2 diabetic nephropathy. METHODS: According to their urinary albumin-to-creatinine ratio (uACR, 107 patients with type 2 diabetes (eGFR >60 ml/min were divided into normal albuminuria group (DN0 group, 38 cases, microalbuminuria group (DN1 group, 38 cases, and macroalbuminuria group (DN2 group, 31 cases, compared with matched healthy normal control group (NC group, 30 cases. Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting. Real-time quantitative polymerase chain reaction was used to detect NF-κB p65 mRNA expression and ELISA assay was used to detect the levels of urinary MCP-1 and RANTES. RESULTS: Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group. In particular, the increase of nuclear NF-κB p65 protein and NF-κB p65 mRNA expressions, urinary MCP-1/Cr and RANTES/Cr all correlated with the severity of type 2 diabetic nephropathy as indicated by the increase in uACR. Pearson correlation analysis indicated that both urinary MCP-1/Cr and RANTES/Cr were positively correlated with nuclear NF-κB p65 protein or NF-κB p65 mRNA levels. Stepwise multiple regression analysis showed that nuclear NF-κB p65 protein or NF-κB p65 mRNA was an independent variable for urinary MCP-1/Cr, and MCP-1/Cr and RANTES/Cr were two independent variables for uACR. CONCLUSION: Our research demonstrates that nuclear NF-κB p65 protein and mRNA expressions in ex vivo isolated peripheral blood mononuclear cells well correlate with urinary MCP-1/Cr, RANTES/Cr and the severity of type 2 diabetic nephropathy.

  12. Composite repairs qualification according to ISO/TS 24817

    Energy Technology Data Exchange (ETDEWEB)

    Meniconi, Luiz C.M.; Perrut, Valber A. [Petroleo Brasileiro S.A. (PETROBRAS/CENPES), Rio de Janeiro, RJ (Brazil). Centro de Pesquisas

    2009-07-01

    Composite repairs for metallic pipes from three different suppliers were evaluated according to ISO Technical Specification TS 24817. The intended application scenarios are offshore production plants, but the design methodology is also applicable to onshore pipelines and pipework. The evaluation covered all the relevant mechanical properties and established the maximum application temperatures for each repair system. The tests also considered the application of composite repair sleeves to metallic pipes with through thickness defects, by measuring the strain energy release rates of the composite-metal interfaces. The test campaigns aimed to verify the applicability of the recently published ISO document, and to implement it as the routine procedure for composite repairs evaluation within PETROBRAS. The tests also addressed the influence of metallic pipe surface preparation on the final properties of the repair sleeves, especially in relation to the long term behavior of leaking pipes repaired by means of composite materials. (author)

  13. Why did ISO 9001:2008 system fail to deliver?

    Science.gov (United States)

    Langford, Melvyn

    2014-02-01

    This article is based on an actual investigation undertaken, and summarises the subsequent report's findings and observations. It has been anonymised for obvious reasons. In May 2013 an analysis was undertaken by a multidisciplinary team that compared an NHS Trust estates department's managerial systems against the NHS national recommendations. The conclusions stated that: 'There was a systemic failure across a large number of topics generating intolerable/substantial risks to the organisation, its staff, and patients; this despite the department's managerial systems being accredited to the International Standard ISO 9001:2008'. The natural question raised when presented with this demonstrable and auditable evidence was: 'Why did the ISO 9001:2008 system fail?'

  14. ISO 9000 case study: the role of the external consultant.

    Science.gov (United States)

    Nevalainen, D E

    1998-01-01

    Increasingly, blood centers worldwide are turning toward the ISO (International Organization for Standardization) 9000 Quality System Standards to meet regulatory demands of public authorities, competitive demands within their industry, and cost requirements from their customers. The South Texas Blood and Tissue Center is the first blood center in North America to achieve registration to the ISO 9002 Quality System Standards. The role of the external consultant in initiating the process and as a facilitator during the implementation process is examined. To ensure the long-term success and viability of any quality system, it is necessary for an organization to develop and implement its own program. External consultants can assist in many ways, but it is important that their role be a supporting one rather than one of direct implementation.

  15. Geometry-invariant GRIN lens: iso-dispersive contours.

    Science.gov (United States)

    Bahrami, Mehdi; Goncharov, Alexander V

    2012-07-01

    A dispersive model of a gradient refractive index (GRIN) lens is introduced based on the idea of iso-dispersive contours. These contours have constant Abbe number and their shape is related to iso-indicial contours of the monochromatic geometry-invariant GRIN lens (GIGL) model. The chromatic GIGL model predicts the dispersion throughout the GRIN structure by using the dispersion curves of the surface and the center of the lens. The analytical approach for paraxial ray tracing and the monochromatic aberration calculations used in the GIGL model is employed here to derive closed-form expressions for the axial and lateral color coefficients of the lens. Expressions for equivalent refractive index and the equivalent Abbe number of the homogeneous equivalent lens are also presented and new aspects of the chromatic aberration change due to aging are discussed. The key derivations and explanations of the GRIN lens optical properties are accompanied with numerical examples for the human and animal eye GRIN lenses.

  16. Sistema de gestión ambiental: Serie ISO 14000

    Directory of Open Access Journals (Sweden)

    Rafael Pérez Uribe

    2013-08-01

    Full Text Available La implementación de la Norma ISO 14000 en las organizaciones, implica la toma de conciencia por parte de estas en el tema ambiental. Las empresas deben empezar a entender que la implementación de esta norma está directamente ligada con aspectos económicos, tales como, el máximo aprovechamiento de los recursos, el acceso a nuevos mercados y el posicionamiento de nuevos productos. Este artículo presenta algunas generalidades sobre la Norma ISO 14000 para luego centrarse en estadísticas nacionales e internacionales sobre la cantidad de organizaciones que actualmente tienen implementada la Norma, para finalmente dar algunas recomendaciones con el ánimo de dejar la inquietud, acerca de la importancia que tiene el cumplimiento de esta normativa.

  17. ENVIRONMENTAL RESPONSIBILITY MODEL BASED ON ISO 14000 MANAGEMENT SYSTEMS

    Directory of Open Access Journals (Sweden)

    Catalina SITNIKOV

    2012-01-01

    Full Text Available Worldwide corporations, as well as their stakeholders, are more conscious of the need for environmental management, SR behaviour, and sustainable growth and development. International Standards are becoming more significant for corporations to work towards common environmental management practices. ISO 14001 is the first and the broadest standard intended at a more responsible approach of corporations and the world’s most acknowledged framework for environmental management systems that assists corporations to better manage the effect of their activities on the environment. This article aims to study ISO 14001 implementation and its effects on the environmental responsibility. A model will be built, which covers the environmental management system, the components of organizational culture, being able to influence environmental standards implementation.

  18. Quality control with R an ISO standards approach

    CERN Document Server

    Cano, Emilio L; Prieto Corcoba, Mariano

    2015-01-01

    Presenting a practitioner's guide to capabilities and best practices of quality control systems using the R programming language, this volume emphasizes accessibility and ease-of-use through detailed explanations of R code as well as standard statistical methodologies. In the interest of reaching the widest possible audience of quality-control professionals and statisticians, examples throughout are structured to simplify complex equations and data structures, and to demonstrate their applications to quality control processes, such as ISO standards. The volume balances its treatment of key aspects of quality control, statistics, and programming in R, making the text accessible to beginners and expert quality control professionals alike. Several appendices serve as useful references for ISO standards and common tasks performed while applying quality control with R.

  19. Vocational High School Effectiveness Standard ISO 9001: 2008 for Achievement Content Standards, Standard Process and Competency Standards Graduates

    Directory of Open Access Journals (Sweden)

    Yeni Ratih Pratiwi

    2014-06-01

    Full Text Available Efektivitas Sekolah Menengah Kejuruan Berstandar ISO 9001:2008 terhadap Pencapaian Standar Isi, Standar Proses dan Standar Kompetensi Lulusan Abstract: The purpose of this study was to determine differences in the effectiveness of the achievement of the content standards, process standards, and competency standards in vocational already standard ISO 9001: 2008 with CMS that has not been standardized ISO 9001: 2008 both in public schools and private schools. Data collection using the questionnaire enclosed Likert scale models. Analysis of data using one-way ANOVA using SPSS. The results showed: (1 there is no difference in effectiveness between public SMK ISO standard ISO standards with private SMK (P = 0.001; (2 there are differences in the effectiveness of public SMK SMK ISO standards with ISO standards have not (P = 0.000; (3 there are differences in the effectiveness of public SMK ISO standards with private vocational yet ISO standards (P = 0.000; (4 there are differences in the effectiveness of the private vocational school with vocational standard ISO standard ISO country has not (P = 0.015; (5 there are differences in the effectiveness of the private vocational bertandar ISO with private vocational yet standardized ISO (P = 0.000; (6 there was no difference in the effectiveness of public SMK has not been standardized by the ISO standard ISO private SMK yet. Key Words: vocational high school standards ISO 9001: 2008, the standard content, process standards, competency standards Abstrak: Tujuan penelitian ini untuk mengetahui perbedaan efektivitas pencapaian standar isi, standar proses, dan standar kompetensi lulusan pada SMK yang sudah berstandar ISO 9001:2008 dengan SMK yang belum berstandar ISO 9001:2008 baik pada sekolah negeri maupun sekolah swasta. Pengumpulan data menggunakan kuisioner tertutup model skala likert. Analisis data menggunakan ANOVA one way menggunakan program SPSS. Hasil penelitian menunjukkan: (1 ada perbedaan

  20. Triptolide Transcriptionally Represses HER2 in Ovarian Cancer Cells by Targeting NF-κB

    Directory of Open Access Journals (Sweden)

    Chien-Chih Ou

    2012-01-01

    Full Text Available Triptolide (TPL inhibits the proliferation of a variety of cancer cells and has been proposed as an effective anticancer agent. In this study, we demonstrate that TPL downregulates HER2 protein expression in oral, ovarian, and breast cancer cells. It suppresses HER2 protein expression in a dose- and time-dependent manner. Transrepression of HER2 promoter activity by TPL is also observed. The interacting site of TPL on the HER2 promoter region is located between −207 and −103 bps, which includes a putative binding site for the transcription factor NF-κB. Previous reports demonstrated that TPL suppresses NF-κB expression. We demonstrate that overexpression of NF-κB rescues TPL-mediated suppression of HER2 promoter activity and protein expression in NIH3T3 cells and ovarian cancer cells, respectively. In addition, TPL downregulates the activated (phosphorylated forms of HER2, phosphoinositide-3 kinase (PI3K, and serine/threonine-specific protein kinase (Akt. TPL also inhibits tumor growth in a mouse model. Furthermore, TPL suppresses HER2 and Ki-67 expression in xenografted tumors based on an immunohistochemistry (IHC assay. These findings suggest that TPL transrepresses HER2 and suppresses the downstream PI3K/Akt-signaling pathway. Our study reveals that TPL can inhibit tumor growth and thereby may serve as a potential chemotherapeutic agent.

  1. ISOS: A job evaluation system to implement comparable worth

    Directory of Open Access Journals (Sweden)

    Albert Corominas

    2008-01-01

    Full Text Available A fair pay structure is an essential element of the personnel policy of a firm. If the pay structure is perceived as arbitrary by the members of the staff, it becomes a cause of disturbance of the labor relations. Particularly, a pay structure is unfair if it discriminates against women. Job evaluation is a traditional tool used by companies to assist in the process of determining pay structures that can be also useful to detect and combat wage discrimination, since allow determining whether two jobs are of comparable worth or not. Although there are many kinds of systems, authors agree when defining point factor methods as the most appropriate and fair job evaluation systems. However, even being well defined from a technical point of view, most existing systems give discriminatory results regarding to gender. ISOS, a new job evaluation system which is described in this paper, has been designed, with the aim to define a neutral system with regard to gender, based on present jobs characteristics, existing job evaluation systems and job description questionnaires, international experts’ knowledge and a wide body of literature on gender discrimination and its relation with job evaluation. Using ISOS can contribute to detect, combat and eliminate part of the existing wage discrimination in general and, in particular, against women. ISOS includes all aspects of the work so no characteristics are omitted. The system can be applied in any company and to evaluate any job, and offers flexibility to be adapted to the specific characteristics of an organization. ISOS can also be used to detect and combat wage discrimination. Furthermore, characteristics of present jobs, such as cross-training or flexible working time, are also included so the system can be considered innovative in a very traditional field of industrial engineering.

  2. Flavour data constraints on new physics and SuperIso

    CERN Document Server

    Mahmoudi, F

    2012-01-01

    We discuss the implications of B_s -> mu+mu- and B -> K*mu+mu- decays in the context of indirect searches for new physics, emphasising the new LHCb results. In particular, we derive the consequences of the MFV hypothesis and discuss the importance of the MFV predictions. The impact of the recent LHCb measurements in the context of the MSSM will also be addressed, and the SuperIso program will be briefly described.

  3. An Utilization Method Cooperating ISO drawings and Bookmarks for NDE

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Hyun-Ju; Cho, Chan-Hee; Lee, Tae-Hun [KHNP Central Research Institute, Daejeon (Korea, Republic of)

    2015-05-15

    The inspection of weld parts are performed with various non-destructive test methods such as AUT, VT, PT, ECT, etc. The results have been published in the report booklets. Moreover, huge amount of non-destructive inspection data and reports, which are produced from the '78 Kori 1 to newly constructed power plant, are stored in the management department and DDCC of each power plant. Because the data are not classified, it takes much time to find the corresponding non-destructive test results report of a specific unit of a power plant for a particular year. In addition, it is possibility to make human error because the report is written and submitted after finding manually the non-destructive test results for the concerning weld. When there is a dispute of the results of non-destructive inspection of a particular weld, analyzes the control center, a result of the power plant and the corresponding non-destructive inspection of a particular weld of ISO drawing in many places such as control center, corresponding power plant and CRI was discussed together will be to derive the final conclusion. For discussion, the person in charge of each site, looking for ISO drawings there is a result of the weld, in the past results history report of non-destructive testing for this, by searching the results of the welding site, telephone and E-mail disadvantage of complicated procedures if necessary to the discussion together in -mail is performed I have. In this paper, we describe the contents introduced the ISO drawings and Bookmark function is trying to complement these drawbacks. By applying the present invention utilization, find the ISO drawings manually when querying the results of the non-destructive inspection of past weld, which reduces the time to grasp the content of the non-destructive test results report.

  4. JCAHO accreditation and ISO 9000 certification: an update.

    Science.gov (United States)

    1998-10-01

    With hospital security now considered by the JCAHO as part of the hospital's total environment of care, security directors must be prepared to answer questions about areas where multidisciplinary solutions are sought. At the same time, it may pay to become familiar with ISO 9000 certification, quality standards that may in the future complement JCAHO accreditation. In this report, we'll provide detailed information that may stand you in good stead in the years ahead.

  5. ISO 9000 quality standards: a model for blood banking?

    Science.gov (United States)

    Nevalainen, D E; Lloyd, H L

    1995-06-01

    The recent American Association of Blood Banks publications Quality Program and Quality Systems in the Blood Bank and Laboratory Environment, the FDA's draft guidelines, and recent changes in the GMP regulations all discuss the benefits of implementing quality systems in blood center and/or manufacturing operations. While the medical device GMPs in the United States have been rewritten to accommodate a quality system approach similar to ISO 9000, the Center for Biologics Evaluation and Research of the FDA is also beginning to make moves toward adopting "quality systems audits" as an inspection process rather than using the historical approach of record reviews. The approach is one of prevention of errors rather than detection after the fact (Tourault MA, oral communication, November 1994). The ISO 9000 series of standards is a quality system that has worldwide scope and can be applied in any industry or service. The use of such international standards in blood banking should raise the level of quality within an organization, among organizations on a regional level, within a country, and among nations on a worldwide basis. Whether an organization wishes to become registered to a voluntary standard or not, the use of such standards to become ISO 9000-compliant would be a move in the right direction and would be a positive sign to the regulatory authorities and the public that blood banking is making a visible effort to implement world-class quality systems in its operations. Implementation of quality system standards such as the ISO 9000 series will provide an organized approach for blood banks and blood bank testing operations. With the continued trend toward consolidation and mergers, resulting in larger operational units with more complexity, quality systems will become even more important as the industry moves into the future.(ABSTRACT TRUNCATED AT 250 WORDS)

  6. Di-iso-Butyl Phthalate MATERNAL AND FETAL DATA FROM ...

    Science.gov (United States)

    this file contains the raw data on the effects of in utero administration of di-iso-butyl phthalate on maternal weight gain during dosing and the numbers of fetuses and fetal resorptions. The data have all been previously published, as described on the file metadata sheet. Raw data file from our published studies on DIBP specifically requested (6/14/2016) by NCEA scientists for analysis and inclusion in their assessment of this chemical.

  7. What matters for long-term absenteeism: Iso-Strain at work or at home?

    OpenAIRE

    Kittel, France; Casini, Annalisa; Clays, Els; Godin, Isabelle

    2011-01-01

    OBJECTIVES :To determine the prospective impact of Iso-Strain at Work (Iso-W) and Iso-Strain at Home (Iso-H) in workers, on long term absenteeism. METHODS :In the Belstress III study (N= 2675 workers from 7 enterprises) Job-Demand-Control-Social Support (KARASEK ) as well as Stress problems at Home (KLITZMAN) and Social Support outside work (SYME & BERKMAN) –calculated accordingly in Iso-Strain at Home - were measured besides classical socio-demographic data (age, sex, educational level, prof...

  8. What matters for long-term absenteeism: Iso-Strain at work or at home?

    OpenAIRE

    Kittel, France; Casini, Annalisa; Clays, Els; Godin, Isabelle

    2011-01-01

    OBJECTIVES : To determine the prospective impact of Iso-Strain at Work (Iso-W) and Iso-Strain at Home (Iso-H) in workers, on long term absenteeism. METHODS : In the Belstress III study (N= 2675 workers from 7 enterprises) Job-Demand-Control-Social Support (KARASEK ) as well as Stress problems at Home (KLITZMAN) and Social Support outside work (SYME & BERKMAN) –calculated accordingly in Iso-Strain at Home - were measured besides classical socio-demographic data (age, sex, educational level, pr...

  9. ISO 14051: A new era for MFCA implementation and research

    Directory of Open Access Journals (Sweden)

    Katherine L. Christ

    2016-01-01

    Full Text Available Material flow cost accounting (MFCA is a tool designed to encourage eco-efficiency in organizations by focusing on a reduction in use of materials and related improvements in economic performance of corporations. It provides a way to identify win–win situations where monetary and environmental performance can both be improved. But take-up by business is slow, which seems to go against the notion of strong competition driving economic performance. A recent standard, ISO 14051, has been produced by the International Organization for Standardization, and could bring substantial change to MFCA implementation and research. Drawing on Rogers (2003 theory of diffusion of innovation, and with a focus on the first two stages of the innovation-decision process, knowledge and persuasion, this study sought to analyze MFCA and predict how the 2011 release of ISO 14051 might be expected to influence take-up of MFCA by business, and what this might mean for future research. The analysis revealed that, when combined with ISO involvement, MFCA is well placed in terms of Rogers’ theory, with the future likely to see increased diffusion of MFCA and, as adoption rates increase, more opportunities for research in this area. Specific areas identified as a result of the analysis include: the introduction of new research methods, the need for theoretically informed research, and the potential to address new research questions previously considered impractical.

  10. [ISO 9001 conformity in research, teaching and rehabilitation].

    Science.gov (United States)

    Howorka, K; Kletschka, G; Pumprla, J; Thoma, H

    1998-01-01

    Quality assurance, in particular in the areas of development and production of medical devices, is one of the tasks of biomedical engineering. The interdisciplinary working group "Functional Rehabilitation and Group Education, Vienna" is committed to the development and implementation of group education models on three levels: (1) direct education/instruction of patients with chronic diseases, (2) university research and teaching, and (3) development of technical aids for rehabilitation and the means for disseminating group education models in rehabilitation and therapy. Major aims were, by generating conformity with ISO 9001 standards, to achieve greater transparency and process optimization with very small resources in university (teaching, research, technical aids) and extra-university (rehabilitation) areas. A secondary aim was the establishment of interdisciplinary (clinical and biomedical) cooperation at university level. In all main areas (research, teaching and group education/instruction), ISO 9001-conformity was achieved by our activities on three methodological levels: (1) description and analysis of processes, (2) use of ISO 9001 standards for evaluating internal processes, and (3) optimization measures. The following article contains relevant elements of the quality manual and quality assurance system, and offers a typical example of innovative cooperation between medicine and medical engineering.

  11. Does implementation of ISO standards in hospitals improve patient satisfaction?

    Directory of Open Access Journals (Sweden)

    Vahid Keshtkar

    2017-01-01

    Full Text Available Introduction: Around the world, a large number of projects have been developed with the aim of assessing patient satisfaction especially in hospitals. As an important indicator of the quality of health care system, Patients’ perception of health care has been the center of attention over the recent 20 years. Method: 402 patients who were hospitalized in teaching hospitals affiliated to the Shiraz University of Medical Sciences were investigated. Patients’ satisfactions of the health care services were assessed using the translated and modified version of the KQCAH consisted of 44 questions divided to7 categories of Respect and Caring, Effectiveness and Continuity, Appropriateness, Information, Efficiency, Meals, First Impression, Staff Diversity. All of the patients were asked to fill out the questionnaire (with written informed consents at the time of discharge from the hospitals. Results: Regarding total score of patient satisfaction the ISO-certified hospitals did not show advantages over the uncertified hospitals. The total score of patients’ satisfaction ranged from 66.5 to 77.5 in. Overall, only in one ISO-certified hospital the total score of patient satisfaction representing all dimensions, was significantly higher comparing to other hospitals included in the study. Conclusion: It seems that solitary application of ISO standards could not improve patient satisfaction in hospitals affiliated to Shiraz University of Medical Sciences.

  12. Evaluation of environmental aspects significance in ISO 14001.

    Science.gov (United States)

    Põder, Tõnis

    2006-05-01

    The methodological framework set by standards ISO 14001 and ISO 14004 gives only general principles for environmental aspects assessment, which is regarded as one of the most critical stages of implementing environmental management system. In Estonia, about 100 organizations have been certified to the ISO 14001. Experience obtained from numerous companies has demonstrated that limited transparency and reproducibility of the assessment process serves as a common shortcoming. Despite rather complicated assessment schemes sometimes used, the evaluation procedures have been largely based on subjective judgments because of ill-defined and inadequate assessment criteria. A comparison with some similar studies in other countries indicates a general nature of observed inconsistencies. The diversity of approaches to the aspects' assessment in concept literature and to the related problems has been discussed. The general structure of basic assessment criteria, compatible with environmental impact assessment and environmental risk analysis has also been outlined. Based on this general structure, the article presents a tiered approach to help organize the assessment in a more consistent manner.

  13. Implementation of ISO 17025 in the Eletronuclear Environmental Monitoring Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Baliza, Ana Rosa; Caetano, Carla de Brito, E-mail: baliza@eletronuclear.gov.br [Eletrobrás Termonuclear S.A. (ELETRONUCLEAR), Angra dos Reis, RJ (Brazil)

    2017-07-01

    In order to grant Angra 3 power plant operation license, the Brazilian Institute for the Environment and Renewable Natural Resources (IBAMA), in the IBAMA Installation License 591/2009 condition 2.24, required that Eletronuclear has to obtain the Environmental Monitoring Laboratory accreditation, in accordance with the requirements of NBR ISO / IEC 17025 standard. The accreditation is the formal recognition that a laboratory has a quality assurance system and technical competence to perform specific tests, evaluated according to the criteria based on NBR ISO/IEC 17025 (General requirements for testing and calibration laboratories competence) standard. In Brazil, the General Accreditation Coordination (CGCRE), according to the guidelines already explained by the Brazilian System of Conformity Assessment (INMETRO), does accreditation. The INMETRO is the only official accrediting body in Brazil and internationally recognized by the International Accreditation Forum (IAF). The Environmental Monitoring Laboratory at Eletronuclear is an analytical laboratory of chemical, radiochemical and biological tests, which analyzes several matrices, such as seawater, river water, marine sediment, beach sand, soil, pasture, banana, milk, besides marine sea life, around the Central Almirante Álvaro Alberto power plant station. It is licensed by CNEN, INEA, IBAMA and it follows national and international standards in the performance of the tests as well as in the collection and preparation of samples. This article describes the main steps that the Eletronuclear Environmental Monitoring Laboratory followed to implement ISO 17025. (author)

  14. [Recommendations for implementing the UNE-EN-ISO 15189 quality standard in the clinical microbiology laboratory: bacteriology and serology].

    Science.gov (United States)

    Rojo, María Dolores; Aguiar, Juan Manuel; Cercenado, Emilia; de Ory, Fernando; de la Rosa, Manuel

    2010-11-01

    The UNE-EN-ISO 15189:2007 standard specifies the management and technical requirements that clinical microbiology laboratories must meet to achieve optimal quality when performing microbiological analyses. With implementation of this standard, a laboratory can receive the accreditation and formal recognition of an authorized body, certifying that it is apt for performing an assay or group of assays. In Spain, laboratories that apply these standards can be accredited by the Entidad Nacional de Acreditación (ENAC, Spanish accreditation body). The purpose of this review is to familiarize clinical microbiology laboratory specialists with the UNE-EN-ISO 15189:2007 standard through a practical approach focussed on bacteriology and serology studies. We briefly define the scope and specify the requisites required for managing the quality of the procedures and processes involved in performing tests on human specimens, for document control, and for management of instruments and equipment, personnel, information systems, supply systems, and external services. Lastly, evaluation approaches are indicated to achieve continuing improvement of the processes carried out and the services the laboratory provides. Copyright © 2009 Elsevier España, S.L. All rights reserved.

  15. Evidence of correlation between TGFBR2 gene expression mediated by NF-kB signaling pathways and Kawasaki disease in children.

    Science.gov (United States)

    Gao, Qinling; Yuan, Shuhua; Yuan, Dawei

    2017-09-15

    We explored the correlation between the TGFBR2 gene that is mediated by NF-kb signaling pathways and the pathogenesis of Kawasaki disease in children. In this study, 43 children with Kawasaki disease from April 2014 to January 2016 at our hospital were selected as the observation group, and 42 healthy children were selected as the control group. The mRNA expression levels of NF-kb gene and TGFBR2 gene in different groups were detected using fluorescence quantitative PCR. The protein expression levels of the NF-kb and TGFBR2 were detected using enzyme-linked immunosorbent assay (ELISA) in different groups. The expression levels of NF-kb and TGFBR2 in the observation group and the control group were detected using immunohistochemistry. Compared to the control group, the mRNA expression levels of NF-kb and TGFBR2 were 12.3 times and 27.5 times as high as those in the control group respectively and there were significant differences between the two groups (pKawasaki disease were significantly higher than those in healthy subjects (pKawasaki disease than that in healthy children (4.5%); there was significant difference between the two groups (pKawasaki disease in children through NF-kB signaling pathways.

  16. NF-κB is involved in the regulation of CD154 (CD40 ligand) expression in primary human T cells

    Science.gov (United States)

    Srahna, M; Remacle, J E; Annamalai, K; Pype, S; Huylebroeck, D; Boogaerts, M A; Vandenberghe, P

    2001-01-01

    Cognate interactions between CD154 (CD40 ligand, CD40L) on activated T cells and its receptor CD40 on various antigen-presenting cells are involved in thymus-dependent humoral immune responses and multiple other cell-mediated immune responses. We have studied the regulation of CD154 expression in human T cells after activation with anti-CD3 and anti-CD28 antibodies or after pharmacological activation of protein kinase C with phorbol 12-myristate 13-acetate, and the calcium ionophore ionomycin. Under these conditions, transcription of the CD154 gene was rapidly induced without requiring de novo protein synthesis. Pharmacological inhibitors of NF-κB activation down-regulated CD154 mRNA and protein levels. Cyclosporin A, an inhibitor of NF-AT activation, acted similarly, and the effects of both inhibitors were additive. A potential NF-κB binding site is present within the CD154 promoter at positions −1190 to −1181. In electrophoretic mobility shift assays, this sequence was specifically bound by NF-κB present in nuclear extracts from activated T cells. Furthermore, in transient co-transfection of Jurkat T cells, p65 activated the transcription of a reporter construct containing a multimer of this NF-κB binding site. These observations demonstrate a role of NF-κB transcription factors in the regulation of CD40L expression in activated primary human T cells. PMID:11529914

  17. A novel pathway links oxidative stress to loss of insulin growth factor-2 (IGF2 imprinting through NF-κB activation.

    Directory of Open Access Journals (Sweden)

    Bing Yang

    Full Text Available Genomic imprinting is the allele-specific expression of a gene based on parental origin. Loss of imprinting(LOI of Insulin-like Growth Factor 2 (IGF2 during aging is important in tumorigenesis, yet the regulatory mechanisms driving this event are largely unknown. In this study oxidative stress, measured by increased NF-κB activity, induces LOI in both cancerous and noncancerous human prostate cells. Decreased expression of the enhancer-blocking element CCCTC-binding factor(CTCF results in reduced binding of CTCF to the H19-ICR (imprint control region, a major factor in the allelic silencing of IGF2. This ICR then develops increased DNA methylation. Assays identify a recruitment of the canonical pathway proteins NF-κB p65 and p50 to the CTCF promoter associated with the co-repressor HDAC1 explaining gene repression. An IκBα super-repressor blocks oxidative stress-induced activation of NF-κB and IGF2 imprinting is maintained. In vivo experiments using IκBα mutant mice with continuous NF-κB activation demonstrate increased IGF2 LOI further confirming a central role for canonical NF-κB signaling. We conclude CTCF plays a central role in mediating the effects of NF-κB activation that result in altered imprinting both in vitro and in vivo. This novel finding connects inflammation found in aging prostate tissues with the altered epigenetic landscape.

  18. Collagen gel protects L929 cells from TNFα-induced death by activating NF-κB.

    Science.gov (United States)

    Wang, Hong-Ju; Li, Meng-Qi; Liu, Wei-Wei; Hayashi, Toshihiko; Fujisaki, Hitomi; Hattori, Shunji; Tashiro, Shin-Ichi; Onodera, Satoshi; Ikejima, Takashi

    2017-09-01

    Type I collagen is one of the most abundant components of extracellular matrix. We previously illustrated that murine fibrosarcoma L929 cells grew well on type I collagen gel and escaped from TNFα-induced cell death. In this study, we investigated the mechanism underlying the protective effect of collagen gel. We used western blot, confocal microscopy, MTT assay and flow cytometry by introducing fluorescence staining to determine the expression levels of nuclear factor kappa B (NF-κB), inhibitory ratio and autophagy. L929 cells on collagen gel showed higher expression of NF-κB in the nucleus. Inhibition of NF-κB with pyrrolidine dithiocarbamate hydrochloride (PDTC) or knockdown by NF-κB-siRNA canceled the protective effect of collagen gel on L929 cells from TNFα-induced death, suggesting for the role of NF-κB in the protection from cell death. We found a new aspect of the effect of PDTC on L929 cells cultured on collagen gel. PDTC alone without TNFα induced apoptosis in the L929 cells cultured on collagen gel but not the cells on plastic dish. The apoptosis induction of the L929 cells cultured on collagen gel with PDTC was repressed by inhibiting autophagy with chloroquine, an autophagy inhibitor, suggesting that autophagy contributes to the death induced by the treatment with PDTC. Possible underlying mechanism of this finding is discussed. NF-κB played an important role in protecting the L929 cells cultured on collagen gel from TNFα-induced death.

  19. Cirhin up-regulates a canonical NF-{kappa}B element through strong interaction with Cirip/HIVEP1

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Bin; Mitchell, Grant A. [Genetique Medicale, Centre de Recherche CHU Sainte-Justine, Departement de Pediatrie, Universite de Montreal, Montreal, QC (Canada); Richter, Andrea, E-mail: andrea.richter@umontreal.ca [Genetique Medicale, Centre de Recherche CHU Sainte-Justine, Departement de Pediatrie, Universite de Montreal, Montreal, QC (Canada)

    2009-11-01

    North American Indian childhood cirrhosis (NAIC/CIRH1A) is a severe autosomal recessive intrahepatic cholestasis. All NAIC patients have a homozygous mutation in CIRH1A that changes conserved Arg565 to Trp (R565W) in Cirhin, a nucleolar protein of unknown function. Subcellular localization is unaffected by the mutation. Yeast two-hybrid screening identified Cirip (Cirhin interaction protein) and found that interaction between Cirip and R565W-Cirhin was weakened. Co-immunoprecipitation of the two proteins from nuclear extracts of HeLa cells strongly supports the yeast two hybrid results. Cirip has essentially the same sequence as the C-terminal of HIVEP1, a regulator of a canonical NF-{kappa}B sequence. Since Cirip has the zinc fingers required for this interaction, we developed an in vitro assay based on this element in mammalian cells to demonstrate functional Cirhin-Cirip interaction. The strong positive effect of Cirip on the NF-{kappa}B sequence was further increased by both Cirhin and R565W-Cirhin. Importantly, the effect of R565W-Cirhin was weaker than that of the wild type protein. We observed increased levels of Cirhin-Cirip complex in nuclear extracts in the presence of this NF-{kappa}B sequence. Our hypothesis is that Cirhin is a transcriptional regulatory factor of this NF-{kappa}B sequence and could be a participant in the regulation of other genes with NF-{kappa}B responsive elements. Since the activities of genes regulated through NF-{kappa}B responsive elements are especially important during development, this interaction may be a key to explain the perinatal appearance of NAIC.

  20. NF-κB and CREB are required for angiotensin II type 1 receptor upregulation in neurons.

    Directory of Open Access Journals (Sweden)

    Karla K V Haack

    Full Text Available Nuclear factor kappa B (NF-κB and the Ets like gene-1 (Elk-1 are two transcription factors that have been previously established to contribute to the Angiotensin II mediated upregulation of Angiotensin II type 1 receptor (AT1R in neurons. The cAMP response element binding protein (CREB is another transcription factor that has also been implicated in AT1R gene transcription. The goal of the current study was to determine if NF-κB and CREB association was required for AT1R upregulation. We hypothesized that the transcription of the AT1R gene occurs via an orchestration of transcription factor interactions including NF-κB, CREB, and Elk-1. The synergistic role of CREB and NFκB in promoting AT1R gene expression was determined using siRNA-mediated silencing of CREB. Electrophorectic Mobility Shift Assay studies employing CREB and NF-κB demonstrated increased protein - DNA binding as a result of Ang II stimulation which was blunted by siRNA silencing of CREB. Upstream inhibition of p38 mitogen activated protein kinase (p38 MAPK with SB203580 or inhibition of the calmodulin kinase (CAMK pathway using KN-62 blunted changes in CREB and NF-κB expression. These findings suggest that Ang II may activate multiple signaling pathways involving p38 MAPK leading to the activation of NF-κB and CREB, which feed back to upregulate the AT1R gene. This study provides insight into the molecular mechanisms involving multiple transcription factor activation in a coordinated fashion which may be partially responsible for sympathoexcitation in clinical conditions associated with increased activation of the renin angiotensin system.

  1. Natural Hirudin Increases Rat Flap Viability by Anti-Inflammation via PARs/p38/NF-κB Pathway

    Directory of Open Access Journals (Sweden)

    Liu Peng

    2015-01-01

    Full Text Available The present study aimed to evaluate the effect of natural hirudin on rat random skin flap viability and to determine the mechanism. Forty-eight rats were randomly divided into 2 groups. After the dorsal skin flap operation (3 cm × 10 cm in size, subcutaneous injections of 6 ATU hirudin were administered to group H (n=24 every 12 h, while group C (n=24 received an equal volume of 0.9% normal saline. Six rats from each group were euthanized 1, 2, 4, and 7 days after the operation. A full skin sample was collected from these rats to measure the p38-mitogen-activated protein kinase (p38-MAPK, phospho-p38- (Pp38- MAPK, nuclear factor-κB (NF-κB p65, phosphor-NF-κB (pNF-κB p65, tumour necrosis factor- (TNF- α, interleukin- (IL- 6, and intercellular adhesion molecule- (ICAM- 1 levels via western blot (WB assays. The results showed that flap viability was significantly higher in the hirudin-treated group, which showed a reduced inflammatory response compared with the control group. The Pp38/p38, pNF-κB p65/NF-κB p65, TNF-α, IL-6, and ICAM-1 levels in the hirudin-treated group were lower than those in the control group. The results demonstrated that hirudin could improve random skin flap viability and suggested that this effect maybe occurs by blocking the thrombin/proteinase-activated receptors (PARs/p38/NF-κB signalling pathway, thus decreasing the inflammatory response.

  2. A Cross-Country Empirical Study Towards the Impact of Following ISO Management System Standards on Euro-Area Economic Confidence

    OpenAIRE

    Armeanu, Stefan Daniel; Vintila, Georgeta; Gherghina, Stefan Cristian

    2017-01-01

    This study aims to examine the impact of following ISO management system standards on economic sentiment indicator (ESI) as proxy of economic agents’ general view concerning economic activity, for 21 European Union member states over 2005-2014. The empirical research comprises ISO standards with reference to management systems towards quality (ISO 9001, ISO 13485, ISO 16949), food safety (ISO 22000), environment (ISO 14001), and information security (ISO 27001). Panel data fixed effects regre...

  3. Veronicastrum axillare Alleviates Ethanol-Induced Injury on Gastric Epithelial Cells via Downregulation of the NF-kB Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Wei-chun Zhao

    2017-01-01

    Full Text Available We used human gastric epithelial cells (GES-1 line in an ethanol-induced cell damage model to study the protective effect of Veronicastrum axillare and its modulation to NF-κB signal pathway. The goal was to probe the molecular mechanism of V. axillare decoction in the prevention of gastric ulcer and therefore provide guidance in the clinical application of V. axillare on treating injuries from chronic nephritis, pleural effusion, gastric ulcer, and other ailments. The effects of V. axillare-loaded serums on cell viability were detected by MTT assays. Enzyme-linked immunosorbent assay (ELISA and Real-Time PCR methods were used to analyze the protein and mRNA expression of TNF-α, NF-κB, IκBα, and IKKβ. The results showed that V. axillare-loaded serum partially reversed the damaging effects of ethanol and NF-κB activator (phorbol-12-myristate-13-acetate: PMA and increased cell viability. The protein and mRNA expressions of TNF-α, NF-κB, IκBα, and IKKβ were significantly upregulated by ethanol and PMA while they were downregulated by V. axillare-loaded serum. In summary, V. axillare-loaded serum has significantly protective effect on GES-1 against ethanol-induced injury. The protective effect was likely linked to downregulation of TNF-α based NF-κB signal pathway.

  4. Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF-kappaB.

    Science.gov (United States)

    Bork, P M; Schmitz, M L; Kuhnt, M; Escher, C; Heinrich, M

    1997-01-27

    The potential inhibitory effect of 54 Mexican Indian medicinal plants on the activation of transcription factor NF-kappaB was studied. Band-shift experiments identified the ethanolic leaf extracts of Artemisia ludoviciana ssp. mexicana, Calea zacatechichi, and Polymnia maculata (all rich in sesquiterpene lactones) as inhibitors of NF-kappaB down to a concentration of 25 microg/ml. The sesquiterpene lactones isohelenin and parthenolide prevented NF-kappaB activation completely as low as 5 microM. Treatment of HeLa cells with leaf extract of A. ludoviciana ssp. mexicana, isohelenin and parthenolide prevented the induction of transcription on the IL-6 promoter. These experiments identify the eudesmanolide and germacranolide type of sesquiterpene lactones as potent non-antioxidant inhibitors of NF-kappaB. All plants active in the NF-kappaB assay also showed a delay in the onset of capillary reactions of the allantois membrane in a physiological model for anti-inflammatory activity - the HET-CAM assay.

  5. The influence of motivations for seeking ISO 14001 certification: an empirical study of ISO 14001 certified facilities in Hong Kong.

    Science.gov (United States)

    Fryxell, Gerald E; Szeto, Agnes

    2002-07-01

    This study proposes a model linking firm motivations for seeking ISO 14001 certification to the effectiveness of three major components of an environmental management system (EMS)--environmental policy, the assessment of environmental aspect and impacts, and management review. The relationship of these EMS components with a self-reported measure of environmental performance is also examined. From a sample of 29 ISO 14001 certified facilities in Hong Kong (of 71 total certifications at the time the data was collected), it was found that motivations to enhance firm reputation are positively related to perceptions of the effectiveness of the policy statement. Motivations for reputation enhancement and cost reduction were positively related to perceptions of EMS effectiveness in assessing environmental aspects and impacts. The effectiveness of the methodology for identifying environmental aspects and impacts was also found to be positively related to improved environmental performance.

  6. [Validation of the ISO 15189 trial assessment results of clinical laboratories--effects of accreditation and interpretation of ISO 15189].

    Science.gov (United States)

    Aoyagi, Tsutomu; Kawai, Tadashi

    2006-05-01

    Japanese Committee for Clinical Laboratory Standards (JCCLS) and the Japan Accreditation Board for Conformity Assessment (JAB) have developed an accreditation program for clinical laboratories using ISO 15189 in Japan. On October 1, 2004, twelve clinical laboratories applied for trial accreditation assessment. Seven laboratories were selected, including a university hospital laboratory, two other hospital-based laboratories, three large private clinical laboratories and an other private clinical laboratory. JAB had given a one week-training course to the candidate technical assessors who were nominated from the Japanese Society of Laboratory Medicine (JSLM) and the Japanese Association of Medical Technologists (JAMT), and candidate system assessors who were nominated from JAB. Thirty-five members had successfully passed the examination. During the period from November 2004 to May 2005, five laboratories were visited for assessment by four to six assessors for two or three days. Final assessment reports were discussed by the program developing committee for the validation of the accreditation program. The conclusions were that the program did not have any critical problems, but the assessment visit would have been more efficient if JAB published an easily understandable guidance document on ISO 15189, and had some training courses for laboratories in Japan in order to understand ISO 15189 more deeply. In addition, JAB should develop a more simplified checklist, mainly describing the important requirements. JAB formally started the clinical laboratory accreditation scheme from August 1, 2005 and five laboratories were accredited by the newly started accreditation committee on August 31, 2005, and four laboratories were additionally accredited on November 28, 2005. The most difficult clauses in ISO 15189:2003 were 5.6.2 and 5.6.3 on uncertainty and traceability, and 5.7.1 on post-examination procedures. Some difficult clauses to understand in the International

  7. Ciliary neurotrophic factor activates NF-κB to enhance mitochondrial bioenergetics and prevent neuropathy in sensory neurons of streptozotocin-induced diabetic rodents.

    Science.gov (United States)

    Saleh, Ali; Roy Chowdhury, Subir K; Smith, Darrell R; Balakrishnan, Savitha; Tessler, Lori; Martens, Corina; Morrow, Dwane; Schartner, Emily; Frizzi, Katie E; Calcutt, Nigel A; Fernyhough, Paul

    2013-02-01

    Diabetes causes mitochondrial dysfunction in sensory neurons that may contribute to peripheral neuropathy. Ciliary neurotrophic factor (CNTF) promotes sensory neuron survival and axon regeneration and prevents axonal dwindling, nerve conduction deficits and thermal hypoalgesia in diabetic rats. In this study, we tested the hypothesis that CNTF protects sensory neuron function during diabetes through normalization of impaired mitochondrial bioenergetics. In addition, we investigated whether the NF-κB signal transduction pathway was mobilized by CNTF. Neurite outgrowth of sensory neurons derived from streptozotocin (STZ)-induced diabetic rats was reduced compared to neurons from control rats and exposure to CNTF for 24 h enhanced neurite outgrowth. CNTF also activated NF-κB, as assessed by Western blotting for the NF-κB p50 subunit and reporter assays for NF-κB promoter activity. Conversely, blockade of NF-κB signaling using SN50 peptide inhibited CNTF-mediated neurite outgrowth. Studies in mice with STZ-induced diabetes demonstrated that systemic therapy with CNTF prevented functional indices of peripheral neuropathy along with deficiencies in dorsal root ganglion (DRG) NF-κB p50 expression and DNA binding activity. DRG neurons derived from STZ-diabetic mice also exhibited deficiencies in maximal oxygen consumption rate and associated spare respiratory capacity that were corrected by exposure to CNTF for 24 h in an NF-κB-dependent manner. We propose that the ability of CNTF to enhance axon regeneration and protect peripheral nerve from structural and functional indices of diabetic peripheral neuropathy is associated with targeting of mitochondrial function, in part via NF-κB activation, and improvement of cellular bioenergetics. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Phosphorylated neurofilament subunit NF-H becomes elevated in the cerebrospinal fluid of patients with acutely worsening symptoms of compression myelopathy.

    Science.gov (United States)

    Takahashi, Hiroshi; Aoki, Yasuchika; Nakajima, Arata; Sonobe, Masato; Terajima, Fumiaki; Saito, Masahiko; Taniguchi, Shinji; Yamada, Manabu; Watanabe, Fusako; Furuya, Takeo; Koda, Masao; Yamazaki, Masashi; Takahashi, Kazuhisa; Nakagawa, Koichi

    2014-12-01

    It is known that the severity of compression myelopathy sometimes worsens rapidly and results in poor functional recovery because of limited axonal regeneration. Levels of phosphorylated neurofilament subunit NF-H (pNF-H), which indicate axonal degeneration, are elevated in other neurological disorders. To our knowledge, there has been no examination of pNF-H levels in compression myelopathy. Therefore, we conducted a pilot cross-sectional study to evaluate pNF-H levels in the cerebrospinal fluid (CSF) of patients with worsening symptoms of cervical compression myelopathy. From January 2011 to March 2013, 51 samples of CSF were collected from patients at the time of myelography before spinal surgery. The indications for surgery were acutely worsening compression myelopathy (AM) in eight, chronic compression myelopathy (CM) in six, and lumbar canal stenosis (LCS) in 37 patients. The pNF-H levels were measured using a standard enzyme-linked immunosorbent assay. The mean ± standard deviation pNF-H value was 2127.1 ± 556.8 pg/ml in AM patients, 175.8 ± 67.38 pg/ml in CM patients and 518.7 ± 665.7 pg/ml in LCS patients. A significant increase in pNF-H levels was detected in the CSF of patients with AM compared with those with either CM or LCS. The clinical outcome of surgical treatment for patients with cervical myelopathy was satisfactory in both AM and CM patients. Despite the limitations of small sample size and lack of healthy CSF control data due to ethical considerations, our results suggest that pNF-H in CSF can act as a biomarker that reflects the severity of AM. Copyright © 2014. Published by Elsevier Ltd.

  9. Attenuation of liver pro-inflammatory responses by Zingiber officinale via inhibition of NF-kappa B activation in high-fat diet-fed rats.

    Science.gov (United States)

    Li, Xiao-Hong; McGrath, Kristine C-Y; Nammi, Srinivas; Heather, Alison K; Roufogalis, Basil D

    2012-03-01

    The aim of this study was to investigate whether treatment with a ginger (Zingiber officinale) extract of high-fat diet (HFD)-fed rats suppresses Nuclear factor-kappa B (NF-κB)-driven hepatic inflammation and to subsequently explore the molecular mechanisms in vitro. Adult male Sprague-Dawley rats were treated with an ethanolic extract of Zingiber officinale (400 mg/kg) along with a HFD for 6 weeks. Hepatic cytokine mRNA levels, cytokine protein levels and NF-κB activation were measured by real-time PCR, Western blot and an NF-κB nuclear translocation assay, respectively. In vitro, cell culture studies were carried out in human hepatocyte (HuH-7) cells by treatment with Zingiber officinale (100 μg/mL) for 24 hr prior to interleukin-1β (IL-1β, 8 ng/mL)-induced inflammation. We showed that Zingiber officinale treatment decreased cytokine gene TNFα and IL-6 expression in HFD-fed rats, which was associated with suppression of NF-κB activation. In vitro, Zingiber officinale treatment decreased NF-κB-target inflammatory gene expression of IL-6, IL-8 and serum amyloid A1 (SAA1), while it suppressed NF-κB activity, IκBα degradation and IκB kinase (IKK) activity. In conclusion, Zingiber officinale suppressed markers of hepatic inflammation in HFD-fed rats, as demonstrated by decreased hepatic cytokine gene expression and decreased NF-κB activation. The study demonstrates that the anti-inflammatory effect of Zingiber officinale occurs at least in part through the NF-κB signalling pathway. © 2011 The Authors. Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society.

  10. The Accomplices of NF-κB Lead to Radioresistance.

    Science.gov (United States)

    Bai, Miaomiao; Ma, Xiaoxing; Li, Xiaolei; Wang, Xiaohui; Mei, Qian; Li, Xiang; Wu, Zhiqiang; Han, Weidong

    2015-01-01

    Ionizing radiation (IR) plays an important role in the treatment of epithelial tumors, such as lung and prostate cancer, by wounding and killing cancer cells. However, IR also activates sophisticated anti-apoptotic transcriptional factors such that cancer cells fail to repair DNA damage and obtain resistance to apoptosis under conditions of radiotherapy. Among these transcription factors, the transcription factor nuclear factor kappa B (NF-κB) is recognized as a key feature for protecting cells from apoptosis in most cell types. Moreover, the induction of radioresistance is mediated by several genes that are regulated by NF- κB. The primary purpose of this review is to introduce the studies of the signaling mechanisms of IR in NF-κB activation, such as ROS/NF-κB, ATM or DNA-PK/MAPKK/ p90rsk, PI3K/AKT/IKK and k-ras/c-raf/ MEKK/ NF-κB pathways. Moreover, we describe how the expression of the target genes (e.g., XIAP, A20, FLIP, Bcl-xL) are induced by NF-κB to regulate the activation of survival signaling pathways and to inhibit apoptotic signaling pathways. In addition, IR activates NF-κB to express cell cycle-specific genes, for example cyclin D1, which is associated with reinforcing radioresistance. We exhibit the signaling pathways that are induced by IR stimulation of NF-κB and illustrate the molecular mechanisms of radioresistance.

  11. [NF-kappaB tumorigenesis and drug developement].

    Science.gov (United States)

    Liu, Wei-Feng; Yu, Shan-Shan; Li, Yue-Zhong

    2005-01-01

    NF-kappaB, a collective name of dimeric transcription factors, is composed of members of the Rel family proteins that recognize and bind a specific DNA sequence. It is normally sequestered in the cytoplasm of non-stimulated cells by associating with a family of inhibitor proteins called IkappaBs. Exposure of cells to a variety of extra-and intra-cellular stimuli leads to the rapid proteolytic degradation of IkappaBs, which frees NF-kappaBs allowing them to translocate to the nucleus where it regulates gene transcription. NF-kappaB is involved in a lot of physiological processes such as immunity, inflammation, cell proliferation, apoptosis and even tumorigenesis by regulating the transcription of a larger number of genes. This review introduces the various mechanisms of NF-kappaB activation including a recently reported alternative activation pathway mediated by lymphotoxin alpha/beta, B cell activating factor and CD40 ligand. The signal transduction pathway leading to NF-kappaB activation via IKK in response to proinflammatory factors like TNF-alpha and IL-1 is addressed in more detail concerning the regulation of IKK activity, mechanism of IkappaB degradation and regulation of transactivation activity of NF-kappaB on different levels. Considering the important role of NF-kappaB in cell proliferation and regulation of various genes participating in apoptosis, the involvement of NF-kappaB in tumorigenesis and drug screening is also discussed.

  12. NF-κB Signaling in Gastric Cancer

    Science.gov (United States)

    Sokolova, Olga; Naumann, Michael

    2017-01-01

    Gastric cancer is a leading cause of cancer death worldwide. Diet, obesity, smoking and chronic infections, especially with Helicobacter pylori, contribute to stomach cancer development. H. pylori possesses a variety of virulence factors including encoded factors from the cytotoxin-associated gene pathogenicity island (cagPAI) or vacuolating cytotoxin A (VacA). Most of the cagPAI-encoded products form a type 4 secretion system (T4SS), a pilus-like macromolecular transporter, which translocates CagA into the cytoplasm of the host cell. Only H. pylori strains carrying the cagPAI induce the transcription factor NF-κB, but CagA and VacA are dispensable for direct NF-κB activation. NF-κB-driven gene products include cytokines/chemokines, growth factors, anti-apoptotic factors, angiogenesis regulators and metalloproteinases. Many of the genes transcribed by NF-κB promote gastric carcinogenesis. Since it has been shown that chemotherapy-caused cellular stress could elicit activation of the survival factor NF-κB, which leads to acquisition of chemoresistance, the NF-κB system is recommended for therapeutic targeting. Research is motivated for further search of predisposing conditions, diagnostic markers and efficient drugs to improve significantly the overall survival of patients. In this review, we provide an overview about mechanisms and consequences of NF-κB activation in gastric mucosa in order to understand the role of NF-κB in gastric carcinogenesis. PMID:28350359

  13. Gestion de la calidad y del medio ambiente en instituciones de educacion superior mediante integracion de ISO 9001 e ISO 14001

    National Research Council Canada - National Science Library

    Tlapa, Diego A; Limon, Jorge; Baez, Yolanda A

    2009-01-01

    ... la factibilidad de la integracion de gestion de la calidad y medio ambiente. Para ello, se realizo una busqueda de instituciones de educacion superior que trabajan bajo ISO 9001 e ISO 14001, encontrando 322 y 3 certificados respectivamente en el ano 2008...

  14. A practical synthesis of long-chain iso-fatty acids (iso-C12-C19) and related natural products.

    Science.gov (United States)

    Richardson, Mark B; Williams, Spencer J

    2013-01-01

    A gram-scale synthesis of terminally-branched iso-fatty acids (iso-C12-C19) was developed commencing with methyl undec-10-enoate (methyl undecylenate) (for iso-C12-C14) or the C15 and C16 lactones pentadecanolide (for iso-C15-C17) and hexadecanolide (for iso-C18-C19). Central to the approaches outlined is the two-step construction of the terminal isopropyl group through addition of methylmagnesium bromide to the ester/lactones and selective reduction of the resulting tertiary alcohols. Thus, the C12, C17 and C18 iso-fatty acids were obtained in three steps from commercially-available starting materials, and the remaining C13-C16 and C19 iso-fatty acids were prepared by homologation or recursive dehomologations of these fatty acids or through intercepting appropriate intermediates. Highlighting the synthetic potential of the iso-fatty acids and various intermediates prepared herein, we describe the synthesis of the natural products (S)-2,15-dimethylpalmitic acid, (S)-2-hydroxy-15-methylpalmitic acid, and 2-oxo-14-methylpentadecane.

  15. A practical synthesis of long-chain iso-fatty acids (iso-C12–C19 and related natural products

    Directory of Open Access Journals (Sweden)

    Mark B. Richardson

    2013-09-01

    Full Text Available A gram-scale synthesis of terminally-branched iso-fatty acids (iso-C12–C19 was developed commencing with methyl undec-10-enoate (methyl undecylenate (for iso-C12–C14 or the C15 and C16 lactones pentadecanolide (for iso-C15–C17 and hexadecanolide (for iso-C18–C19. Central to the approaches outlined is the two-step construction of the terminal isopropyl group through addition of methylmagnesium bromide to the ester/lactones and selective reduction of the resulting tertiary alcohols. Thus, the C12, C17 and C18 iso-fatty acids were obtained in three steps from commercially-available starting materials, and the remaining C13–C16 and C19 iso-fatty acids were prepared by homologation or recursive dehomologations of these fatty acids or through intercepting appropriate intermediates. Highlighting the synthetic potential of the iso-fatty acids and various intermediates prepared herein, we describe the synthesis of the natural products (S-2,15-dimethylpalmitic acid, (S-2-hydroxy-15-methylpalmitic acid, and 2-oxo-14-methylpentadecane.

  16. NF1 germline mutation differentially dictates optic glioma formation and growth in neurofibromatosis-1

    OpenAIRE

    Toonen, Joseph A.; Anastasaki, Corina; Smithson, Laura J.; Gianino, Scott M.; Li, Kairong; Kesterson, Robert A.; Gutmann, David H.

    2016-01-01

    Neurofibromatosis type 1 (NF1) is a common neurogenetic condition characterized by significant clinical heterogeneity. A major barrier to developing precision medicine approaches for NF1 is an incomplete understanding of the factors that underlie its inherent variability. To determine the impact of the germline NF1 gene mutation on the optic gliomas frequently encountered in children with NF1, we developed genetically engineered mice harboring two representative NF1-patient-derived Nf1 gene m...

  17. 78 FR 67357 - New England Power Generators Association, Inc. v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2013-11-12

    ... Energy Regulatory Commission New England Power Generators Association, Inc. v. ISO New England Inc... Association, Inc. (NEPGA or Complainant) filed a complaint against ISO New England, Inc. (ISO-NE or Respondent). NEPGA alleges that the provisions of the ISO-NE Tariff that set capacity prices during circumstances...

  18. The Arabidopsis NF-YA3 and NF-YA8 genes are functionally redundant and are required in early embryogenesis.

    Directory of Open Access Journals (Sweden)

    Monica Fornari

    Full Text Available Nuclear factor Y (NF-Y is a trimeric transcription factor composed of three distinct subunits called NF-YA, NF-YB and NF-YC. In Arabidopsis thaliana, NF-Y subunits are known to play roles in many processes, such as gametogenesis, embryogenesis, seed development, drought resistance, ABA signaling, flowering time, primary root elongation, Endoplasmic Reticulum (ER stress response and blue light responses. Here, we report that the closely related NF-YA3 and NF-YA8 genes control early embryogenesis. Detailed GUS and in situ analyses showed that NF-YA3 and NF-YA8 are expressed in vegetative and reproductive tissues with the highest expression being during embryo development from the globular to the torpedo embryo stage. Plants from the nf-ya3 and nf-ya8 single mutants do not display any obvious phenotypic alteration, whereas nf-ya3 nf-ya8 double mutants are embryo lethal. Morphological analyses showed that the nf-ya3 nf-ya8 embryos fail to undergo to the heart stage and develop into abnormal globular embryos with both proembryo and suspensor characterized by a disordered cell cluster with an irregular shape, suggesting defects in embryo development. The suppression of both NF-YA3 and NF-YA8 gene expression by RNAi experiments resulted in defective embryos that phenocopied the nf-ya3 nf-ya8 double mutants, whereas complementation experiments partially rescued the abnormal globular nf-ya3 nf-ya8 embryos, confirming that NF-YA3 and NF-YA8 are required in early embryogenesis. Finally, the lack of GFP expression of the auxin responsive DR5rev::GFP marker line in double mutant embryos suggested that mutations in both NF-YA3 and NF-YA8 affect auxin response in early developing embryos. Our findings indicate that NF-YA3 and NF-YA8 are functionally redundant genes required in early embryogenesis of Arabidopsis thaliana.

  19. Isolation of anteiso-C17, iso-C17, iso-C16, and iso-C15 bacillomycin D from Bacillus amyloliquefaciens SD-32 and their antifungal activities against plant pathogens.

    Science.gov (United States)

    Tanaka, Keijitsu; Ishihara, Atsushi; Nakajima, Hiromitsu

    2014-02-19

    Two new cyclic lipopeptides (3 and 4) were isolated from the culture filtrate of Bacillus amyloliquefaciens strain SD-32, together with two known metabolites, iso-C15 and iso-C16 bacillomycin D (1 and 2). Spectroscopic and chemical analyses identified the structures of the new compounds 3 and 4 as anteiso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-14-methylhexadecanoic acid) and iso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-15-methylhexadecanoic acid), respectively. The absolute configuration of C-3 in the β-amino fatty acid was determined to be R on the basis of the CD spectrum of its dinitrophenyl-p-methoxyaniline derivative. The activities of compounds 1-4 were evaluated against 13 plant pathogens: the activities of anteiso- and iso-C17 bacillomycin D (3 and 4) were almost the same and stronger than those of iso-C15 and iso-C16 bacillomycin D (1 and 2); iso-C15 bacillomycin D (1) was weakest. Compounds 2-4 inhibited the growth of all fungi tested; however, Pythium aphanidermatum was not inhibited at all by any of the compounds. Furthermore, compounds 1-4 at concentrations of 80, 40, 30, and 30 μM, respectively, inhibited completely the Botrytis cinerea infection in cucumber leaf.

  20. A novel intestinal trans-factor (NF-LPH1) interacts with the lactase-phlorizin hydrolase promoter and co-varies with the enzymatic activity

    DEFF Research Database (Denmark)

    Troelsen, J T; Norén, O; Sjöström, H

    1992-01-01

    The promoter of the pig lactase-phlorizin hydrolase was cloned and showed to be functional in the human intestinal cell line Caco2. The proximal promoter was analyzed for binding of nuclear proteins from small intestine and liver. DNase I footprinting and electrophoretic mobility shift assays show...... not contain lactase activity, and NF-LPH1 is not present in liver nuclear extracts in detectable amounts. This indicates that NF-LPH1 is involved in the decline of lactase at weaning and may be of importance for the molecular explanation of hypolactasia in humans. It was demonstrated by transfection of two...

  1. The Effect of NF-κB Signalling Pathway on Expression and Regulation of Nacrein in Pearl Oyster, Pinctada fucata.

    Directory of Open Access Journals (Sweden)

    Juan Sun

    Full Text Available Nacrein is the first identified and widely investigated molluscan matrix protein and is considered to play an important role in the shell formation of the pearl oyster, Pinctada fucata. Here, we investigate the effect of the NF-κB signalling pathway on Nacrein gene expression in P. fucata to elucidate the mechanisms involved in shell formation. Inhibition of NF-κB signalling decreased Nacrein promoter-dependent luciferase activity. However, co-transfection of the Nacrein promoter vector with Pf-IKK or Pf-Rel expression plasmids could enhance luciferase activity, thus proving NF-κB signalling could regulate the transcriptional activity of the Nacrein promoter. Gene silencing by RNA interference and subsequent observation of the inner surface of the nacreous layer of oyster shells by SEM, showed that suppression of the gene Pf-Rel lead to a partial inhibition of Nacrein expression, not only at the mRNA level but also at the protein level. The inner surface of the shells became abnormal. Electrophoretic mobility shift assays (EMSAs revealed that Pf-Rel could directly bind to the relative sites of the Nacrein promoter. These results confirm that an important component of the NF-κB signalling pathway, Pf-Rel, can directly bind the Nacrein promoter in P. fucata, and regulate its transcription and shell formation.

  2. Models for the study of skin wound healing. The role of Nrf2 and NF-κB.

    Science.gov (United States)

    Ambrozova, Nikola; Ulrichova, Jitka; Galandakova, Adela

    2017-03-01

    Nrf2 and NF-κB transcription factors act in wound healing via their anti-inflammatory and anti-oxidant effects or through the immune response. Studying this process is a matter of some importance given the high cost of wound treatment. A major contribution in this regard is being made by models that enable investigation of the involvement of multiple factors in wound healing and testing new curative substances. This literature review was carried out via searches in the PubMed and Web of Science databases up to 2016. It covers skin wound healing, available models for its study (part I), the role of Nrf2 and NF-κB, substances that influence them and whether they can be used as markers (part II). Was found that in vitro assays are used for their availability but a holistic view must be established in vivo. In silico approaches are facilitating assessment of a vast amount of research data. Nfr2 and NF-κB play a crucial and reciprocal role in wound healing. Nrf2 controls repair-associated inflammation and protects against excessive accumulation of ROS while Nf-κB activates the innate immune reaction, proliferation and migration of cells, modulates expression of matrix metalloproteinases, secretion and stability of cytokines and growth factors for wound healing.

  3. C26 cancer-induced muscle wasting is IKKβ-dependent and NF-kappaB-independent.

    Directory of Open Access Journals (Sweden)

    Evangeline W Cornwell

    Full Text Available Existing data suggest that NF-kappaB signaling is a key regulator of cancer-induced skeletal muscle wasting. However, identification of the components of this signaling pathway and of the NF-κB transcription factors that regulate wasting is far from complete. In muscles of C26 tumor bearing mice, overexpression of dominant negative (d.n. IKKβ blocked muscle wasting by 69% and the IκBα-super repressor blocked wasting by 41%. In contrast, overexpression of d.n. IKKα or d.n. NIK did not block C26-induced wasting. Surprisingly, overexpression of d.n. p65 or d.n. c-Rel did not significantly affect muscle wasting. Genome-wide mRNA expression arrays showed upregulation of many genes previously implicated in muscle atrophy. To test if these upregulated genes were direct targets of NF-κB transcription factors, we compared genome-wide p65 binding to DNA in control and cachectic muscle using ChIP-sequencing. Bioinformatic analysis of ChIP-sequencing data from control and C26 muscles showed very little p65 binding to genes in cachexia and little to suggest that upregulated p65 binding influences the gene expression associated with muscle based cachexia. The p65 ChIP-seq data are consistent with our finding of no significant change in protein binding to an NF-κB oligonucleotide in a gel shift assay, no activation of a NF-κB-dependent reporter, and no effect of d.n.p65 overexpression in muscles of tumor bearing mice. Taken together, these data support the idea that although inhibition of IκBα, and particularly IKKβ, blocks cancer-induced wasting, the alternative NF-κB signaling pathway is not required. In addition, the downstream NF-κB transcription factors, p65 and c-Rel do not appear to regulate the transcriptional changes induced by the C26 tumor. These data are consistent with the growing body of literature showing that there are NF-κB-independent substrates of IKKβ and IκBα that regulate physiological processes.

  4. Europe's space telescope ISO finds water in distant places

    Science.gov (United States)

    1997-04-01

    Equally striking is ISO's discovery of water vapour in the outer planets, Saturn, Uranus and Neptune. As those chilly planets cannot release water from within, they probably have a supply of water coming from elsewhere in the Solar System. Since ISO went into orbit at the end of 1995, it has used its unique power of analysing infrared rays coming from the Universe to identify water vapour and water ice near dying stars and newborn stars. It has also measured the water vapour steaming from Comet Hale-Bopp. "Before ISO no instrument was capable of detecting water in so many places," comments ESA's director of science, Roger Bonnet. "To start revealing the cosmic history of the Earth's water is a big success for ESA and for the astronomers who use our unique infrared observatory. And ISO's discovery that water is commonplace in the Galaxy will encourage renewed speculation about life that may exist in the vicinity of other stars." Water amid the stars Primaeval hydrogen atoms make water by joining with oxygen atoms that are manufactured within stars, in nuclear reactions occurring towards the end of a star's life. Oxygen from defunct stars enriches the Galaxy, and abundant hydrogen is available to react with it. Although the existence of water in interstellar space is not surprising, the Earth's moist atmosphere makes life difficult for any astronomer who wishes to spot water vapour in the Universe with ground-based instruments. Observations from aircraft and balloons gave early hints of cosmic water, but thorough investigations had to wait for ISO's unhampered view from space. Three of the satellite's instruments, the Short Wavelength Spectrometer (SWS), the Long Wavelength Spectrometer (LWS) and the photometer ISOPHOT operating in spectroscopic mode, take part in the hunt for water. Last year, for example, users of both SWS and LWS reported water vapour in the vicinity of the aged star, W Hydrae, from which oxygen-rich winds blow into space. The bright infrared

  5. Synthesis and Biological Activity of 2',3'-iso-Aryl-abscisic Acid Analogs.

    Science.gov (United States)

    Wan, Chuan; Wang, Mingan; Yang, Dongyan; Han, Xiaoqiang; Che, Chuanliang; Ding, Shanshan; Xiao, Yumei; Qin, Zhaohai

    2017-12-15

    2',3'-iso-Benzoabscisic acid (iso-PhABA), an excellent selective abscisic acid (ABA) analog, was developed in our previous work. In order to find its more structure-activity information, some structural modifications were completed in this paper, including the substitution of phenyl ring and replacing the ring with heterocycles. Thus, 16 novel analogs of iso-PhABA were synthesized and screened with three bioassays, Arabidopsis and lettuce seed germination and rice seedling elongation. Some of them, i.e., 2',3'-iso-pyridoabscisic acid (iso-PyABA) and 2',3'-iso-franoabscisic acid (iso-FrABA), displayed good bioactivities that closed to iso-PhABA and natural (+)-ABA. Some others, for instance, substituted-iso-PhABA, exhibited certain selectivity to different physiological process when compared to iso-PhABA or (+)-ABA. These analogs not only provided new candidates of ABA-like synthetic plant growth regulators (PGRs) for practical application, but also new potential selective agonist/antagonist for probing the specific function of ABA receptors.

  6. Plasma 8-iso-Prostaglandin F2α, a possible prognostic marker in aneurysmal subarachnoid hemorrhage.

    Science.gov (United States)

    Pan, De-Sheng; Yan, Min; Hassan, Muhammad; Fang, Ze-Bin; Chen, Man-Tao

    2017-06-01

    8-iso-Prostaglandin F2α (8-iso-PGF2α) is a potential biomarker of oxidative stress. This study clarified whether plasma 8-iso-PGF2α concentrations were affected and its underlying relevance to prognosis in aneurysmal subarachnoid hemorrhage (aSAH). In this prospective, observational study, a total of 170 controls and 170 aSAH patients were enrolled. Plasma 8-iso-PGF2α concentrations were detected using an ELISA. Severity was assessed by World Federation of Neurological Surgeons (WFNS) scale and modified Fisher grading scale. Clinical outcomes included 6-month mortality and poor outcome referred to as Glasgow outcome scale score of 1-3. As compared to controls, admission plasma 8-iso-PGF2α concentrations were significantly enhanced. Increased concentrations of plasma 8-iso-PGF2α correlated with WFNS scores and modified Fisher scores. 8-iso-PGF2α in plasma was an independent predictor for clinical outcomes. Under ROC curve, the predictive values of 8-iso-PGF2α concentrations resembled those of WFNS scores and modified Fisher scores for clinical outcomes. An elevation in plasma 8-iso-PGF2α concentrations is associated with the severity and poor outcome after aSAH, substantializing 8-iso-PGF2α as a potential prognostic biomarker of aSAH. Copyright © 2017. Published by Elsevier B.V.

  7. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network.

    Directory of Open Access Journals (Sweden)

    Mala V Rao

    Full Text Available Newly synthesized neurofilaments or protofilaments are incorporated into a highly stable stationary cytoskeleton network as they are transported along axons. Although the heavily phosphorylated carboxyl-terminal tail domains of the heavy and medium neurofilament (NF subunits have been proposed to contribute to this process and particularly to stability of this structure, their function is still obscure. Here we show in NF-H/M tail deletion [NF-(H/M(tailΔ] mice that the deletion of both of these domains selectively lowers NF levels 3-6 fold along optic axons without altering either rates of subunit synthesis or the rate of slow axonal transport of NF. Pulse labeling studies carried out over 90 days revealed a significantly faster rate of disappearance of NF from the stationary NF network of optic axons in NF-(H/M(tailΔ mice. Faster NF disappearance was accompanied by elevated levels of NF-L proteolytic fragments in NF-(H/M(tailΔ axons. We conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons.

  8. miR-486 sustains NF-κB activity by disrupting multiple NF-κB-negative feedback loops

    National Research Council Canada - National Science Library

    Song, Libing; Lin, Chuyong; Gong, Hui; Wang, Chanjuan; Liu, Liping; Wu, Jueheng; Tao, Sha; Hu, Bo; Cheng, Shi-Yuan; Li, Mengfeng; Li, Jun

    2013-01-01

    .... Herein, we report that miR-486 directly suppresses NF-κB-negative regulators, CYLD and Cezanne, as well as multiple A20 activity regulators, including ITCH, TNIP-1, TNIP-2 and TNIP-3, resulting in promotion of ubiquitin conjugations in NF-κ...

  9. Mosaic type-1 NF1 microdeletions as a cause of both generalized and segmental neurofibromatosis type-1 (NF1).

    Science.gov (United States)

    Messiaen, Ludwine; Vogt, Julia; Bengesser, Kathrin; Fu, Chuanhua; Mikhail, Fady; Serra, Eduard; Garcia-Linares, Carles; Cooper, David N; Lazaro, Conxi; Kehrer-Sawatzki, Hildegard

    2011-02-01

    Mosaicism is an important feature of type-1 neurofibromatosis (NF1) on account of its impact upon both clinical manifestations and transmission risk. Using FISH and MLPA to screen 3500 NF1 patients, we identified 146 individuals harboring gross NF1 deletions, 14 of whom (9.6%) displayed somatic mosaicism. The high rate of mosaicism in patients with NF1 deletions supports the postulated idea of a direct relationship between the high new mutation rate in this cancer predisposition syndrome and the frequency of mosaicism. Seven of the 14 mosaic NF1 deletions were type-2, whereas four were putatively type-1, and three were atypical. Two of the four probable type-1 deletions were confirmed as such by breakpoint-spanning PCR or SNP analysis. Both deletions were associated with a generalized manifestation of NF1. Independently, we identified a third patient with a mosaic type-1 NF1 deletion who exhibited segmental NF1. Together, these three cases constitute the first proven mosaic type-1 deletions so far reported. In two of these three mosaic type-1 deletions, the breakpoints were located within PRS1 and PRS2, previously identified as hotspots for nonallelic homologous recombination (NAHR) during meiosis. Hence, NAHR within PRS1 and PRS2 is not confined to meiosis but may also occur during postzygotic mitotic cell cycles. © 2011 Wiley-Liss, Inc.

  10. ISO14000と京都教育大学

    OpenAIRE

    小倉,真智子; 荒木,光

    1999-01-01

    環境問題は,もはや研究議論の時期ではなく,実践すべき時であるといわれて久しい。しかし,環境についてや,環境学についてや,環境問題についてや,環境問題解決についての研究教育はある程度なされはじめてはいるが,いざ実践となると,なかなかどうすればよいかがわからないが,ISO14000を取得し,それを維持していけば地球環境保全の実践ができるといえよう。京都教育大学が他大学に先駆けてISO14000認証取得をすることは,附属環境教育実践センターを持ち,環境学コースを持つ大学としての責務ではないか。そこでまず,ISO14000sの概要を紹介し,その取得のために,本学で,今できることを考え,本学が取得するに際しての問題点とその解決策について論じた。...

  11. Soluciones en las empresas de TI mediante la aplicación de un sistema de gestión ISO 20000 parte 1 integrado a un sistema ISO 27001 e ISO 9001

    OpenAIRE

    Zubieta Guillén, José María

    2010-01-01

    En este proyecto fin de carrera se pretende exponer el beneficio en la gestión de las empresas de TI (Tecnologías de la Información) de los sistemas de gestión integrados ISO 9001 e ISO 27001 tras implantar el complemento ISO 20000 parte 1. A tal efecto se mostrará una empresa de software estándar (para los ejemplos a desarrollar se empleará una empresa encargada del desarrollo, personalización y mantenimiento de un ERP). Ingeniería Técnica Industrial Industria Ingeniaritza Teknikoa...

  12. Choosing change strategy for ISO/IEC 33014

    DEFF Research Database (Denmark)

    Pries-Heje, Jan; Johansen, Jørn

    2015-01-01

    The ISO/IEC 33014.2013 [1] standard on process improvement includes a core activity called ‘identify the overall change strategy’, which includes selecting a change strategy among the many available. This selection can be carried out using the ImprovAbility model [2] in which there is a framework...... analyze how they adapt the change strategy to their specific conditions. We conclude that the most often recommended organizational change strategy is optionality followed by three other strategies: specialist-driven, production-organized, and learning-driven....

  13. ISO 9000 and/or Systems Engineering Capability Maturity Model?

    Science.gov (United States)

    Gholston, Sampson E.

    2002-01-01

    For businesses and organizations to remain competitive today they must have processes and systems in place that will allow them to first identify customer needs and then develop products/processes that will meet or exceed the customers needs and expectations. Customer needs, once identified, are normally stated as requirements. Designers can then develop products/processes that will meet these requirements. Several functions, such as quality management and systems engineering management are used to assist product development teams in the development process. Both functions exist in all organizations and both have a similar objective, which is to ensure that developed processes will meet customer requirements. Are efforts in these organizations being duplicated? Are both functions needed by organizations? What are the similarities and differences between the functions listed above? ISO 9000 is an international standard of goods and services. It sets broad requirements for the assurance of quality and for management's involvement. It requires organizations to document the processes and to follow these documented processes. ISO 9000 gives customers assurance that the suppliers have control of the process for product development. Systems engineering can broadly be defined as a discipline that seeks to ensure that all requirements for a system are satisfied throughout the life of the system by preserving their interrelationship. The key activities of systems engineering include requirements analysis, functional analysis/allocation, design synthesis and verification, and system analysis and control. The systems engineering process, when followed properly, will lead to higher quality products, lower cost products, and shorter development cycles. The System Engineering Capability Maturity Model (SE-CMM) will allow companies to measure their system engineering capability and continuously improve those capabilities. ISO 9000 and SE-CMM seem to have a similar objective, which

  14. The ISO 5-pass authentication in χ-Spaces

    DEFF Research Database (Denmark)

    Cáccamo, Mario J.; Crazzolara, Federico; Milicia, Giuseppe

    2002-01-01

    We implement the ISO 5-pass authentication protocol and study its correctness using the χ-Space framework. χ-Spaces is a novel domain specific programming language designed for developing protocols and in particular security protocols. χ-Spaces programming language is an implementation of SPL...... (Security Protocol Language), a calculus for the description and formal verification of security protocols. χ-Spaces is meant to fill in the gap between a formal specification of a protocol and its implementation, ideally resulitng in the preservation in the implementation of the properties proved...

  15. New guideline represents 'processed-oriented' approach for ISO 9000.

    Science.gov (United States)

    2001-02-01

    Hospitals that have been tracking the development of ISO 9000 in the health care industry should pay close attention to a new guideline about to emerge. According to Laura Preole, health care services manager of SGS International Certification Services based in Rutherford, NJ, the new guideline is the latest step in an effort to establish a more 'process-oriented' method of looking at the health care environment from the moment a patient walks into a facility to the moment he or she is discharged.

  16. Implementation of ISO 9000 to the food industry: an overview.

    Science.gov (United States)

    Efstratiadis, M M; Karirti, A C; Arvanitoyannis, I S

    2000-11-01

    Since the early 1980s, manufacturing industries worldwide have seen a revolution in the way they operate. Consumers have become more and more demanding and the key to company/firm survival is the recognition of customers' satisfaction. In a way, companies have been forced to enhance the quality of their processes and their products. Some of them chose to establish internal quality systems whereas others have opted for employing a general quality system standard, such as the BS 5750 or the ISO 9000 series. In Greece, a great part of the companies (about 80%) employ quality system standards, among which the highest percentage (80%) belong to food manufacturing industries.

  17. Tietoturvallisuuden sertifiointi ISO/IEC 27001 -tietoturvallisuusstandardilla : case: Autovahinkokeskus Oy

    OpenAIRE

    Kemppainen, Simo

    2009-01-01

    Tämän opinnäytetyön aiheena on tutkia tietoturvallisuutta liiketoiminnassa, tietoturvallisuuden hallintajärjestelmän toteuttamista ja ylläpitoa, riskien arviointia sekä tietoturvallisuuden sertifiointivaatimuksia ISO/IEC 27001 -tietoturvallisuusstandardilla. Työssä tutkitaan myös, miten tietoa käsitellään tietoturvallisesti ja miten sitä kuuluu suojata. Autovahinkokeskus Oy on vakuutusyhtiöiden lunastaminen ajoneuvojen realisointia harjoittava yritys, joka työllistää tällä hetkellä 49 hen...

  18. An Initial Comparative Analysis of the CMMI Version 1.2 Development Constellation and the ISO 9000 Family

    Science.gov (United States)

    2009-03-01

    automobile ( ISO TS 16949), chemical (RC 14001 ), medical devices ( ISO 13485), and petroleum and natural gas ( ISO /TS 29001). These individual industry...An Initial Comparative Analysis of the CMMI Version 1.2 Development Constellation and the ISO 9000 Family David H, Kitson, KAMO Consultancy...Credentials Program 10 2.9 Adoption 11 3 Overview of the ISO 9000 Family 13 3.1 Scope of Application and Purpose 13 3.2 Background 13 3.3

  19. GeNF - Experimental report 2006

    Energy Technology Data Exchange (ETDEWEB)

    Pranzas, P.K.; Schreyer, A.; Willumeit, R. (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. of Materials Research

    2007-07-01

    At the Geesthacht Neutron Facility GeNF about 212 experiments were performed in 2006 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guests and by the GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 71 contributions in the present annual experimental report for the year 2006. The contributions may contain one or also several combined experiments. During 2006 the GKSS research reactor FRG-1 achieved an operation time of 197 days at the full 5 MW reactor power providing a neutron flux of ca. 1.4 x 10{sup 14} thermal neutrons/cm{sup 2}s. The cold neutron source was available during the complete operation time. The focus of the in house R and D work at GeNF instruments was the characterisation of nanostructures in engineering materials, the analysis of stresses and textures in welds and technical structures at ARES-2, TEX-2, DCD and SANS-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at PNR, NeRo, POLDI and ROeDI. The thoroughly upgraded residual stress diffractomer ARES-2 went in full operation in spring 2006 as well as the new neutron tomography device at GENRA-3. The installation of modern experiment control hardware and software based on LabView was completed on all designated instruments. In the appendices I and II the experimental reports of REFSANS at FRM II are attached as well as of the GKSS outstation HARWI-II at DESY. Both instruments started full operation in 2006. (orig.)

  20. GeNF - experimental report 2003

    Energy Technology Data Exchange (ETDEWEB)

    Schreyer, A.; Vollbrandt, J.; Willumeit, R. (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. for Materials Research

    2004-07-01

    At the Geesthacht Neutron Facility GeNF about 210 experiments were performed in 2003 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guest and by the GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 76 contributions in the present annual experimental report for the year 2003. The contributions may contain one or also several combined experiments. During 2003 the GKSS research reactor FRG-1 achieved an operation time of 252 days at the full 5 MW reactor power providing a neutron flux of ca. 1,4 x 10{sup 14} thermal neutrons / cm{sup 2} s. The cold neutron source was available during the complete operation time. The focus of the in house R and D work at GeNF instruments was the characterisation of metal alloys, the analysis of stresses in welds and technical structures at ARES, FSS, DCD and SANS-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at PNR and ROeDI. The reflectomer TOREMA was thoroughly upgraded to the instrument NeRo and now offers new measurement possibilities. In the appendices the progress of the project REFSANS at FRM-II is reported as well as the experimental activities of the newly installed GKSS outstation HARWI-II at DESY. (orig.)

  1. Antimetastatic effects of licochalcone B on human bladder carcinoma T24 by inhibition of matrix metalloproteinases-9 and NF-кB activity.

    Science.gov (United States)

    Zhao, Hong; Yuan, Xuan; Jiang, Jiangtao; Wang, Penglong; Sun, Xiling; Wang, Dong; Zheng, Qiusheng

    2014-12-01

    This study investigated the mechanisms by which licochalcone B (LCB) inhibits the adhesion,invasion and metastasis of human malignant bladder cancer T24 cells. Cell viability was evaluated using a sulforhodamine B (SRB) assay. Cell migration and invasion ability were conducted using wound-healing assay and matrigel transwell invasion assay. The activities of matrix metalloproteinases (MMP)-2 and MMP-9 were measured by gelatin zymography protease assays. The expression in protein level of NF-κBP65 and AP-1 was determined using the ELISA method; the protein levels of MMP-9, NF-κBP65, IκBα and P-IκBα were detected by Western blot. The expression in mRNA level of MMP-9 was assessed using quantitative real-time polymerase chain reaction (PCR) and reverse transcription PCR. The results indicated that LCB attenuated T24 cell migration, adhesion and invasion in a concentration-dependent manner. LCB treatment down-regulated the mRNA expression, protein expression and activity of MMP-9 but had no effect on MMP-2. In addition, LCB treatment decreased the protein level of NF-кBP65 and nuclear translocation of NF-кB. These findings suggested that LCB attenuated migration of bladder cancer T24 cells and adhesion and invasion accompanied with down-regulated protein expression of MMP-9 and the nuclear translocation of NF-кB. Our results provide support that LCB may be a potent adjuvant therapeutic agent in the prevention and therapy of bladder cancer. © 2014 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).

  2. ISO 9000 IMPLEMENTATION AND PERCEIVED ORGANISATIONAL OUTCOME: THE CASE OF A SERVICE ORGANISATION

    Directory of Open Access Journals (Sweden)

    Jasni Abdul Latif

    2003-01-01

    Full Text Available The study examines the relationship between the perceptions of the employees about ISO 9000 implementation and perceived organisational outcomes. A conceptual framework linking the level of employee influence and involvement, their perception of ISO 9000 and the perceived impact on their work and the organisational outcomes is posited. Data was collected from a random sample of 64 employees of a service organisation. Contrary to expectations, the work impact of ISO 9000 was not correlated with the efficiency and market outcomes of ISO 9000 certification but the employee involvement, influence and perception of ISO 9000 were. The conceptualization of the influence path of ISO 9000 implementation on the organisational outcomes may require rethinking as perception may have a direct effect on the outcomes without the mediation of the impact on work. In short, employees may report benefits or positive outcomes even though no change is reported in their work.

  3. ISO 14000 information platform. Quarterly report, January 1--March 31, 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-09-01

    GETE, through its ISO 14000 Information Platform, globeNet{trademark} (www.iso14000.net), seeks to provide FETC and the US Department of Energy (DOE) with a timely, accurate, and reliable information resource on the issue of environmental management standards. This resource allows FETC to keep track of the latest developments in the ISO 14000 arena, in DOE as well as throughout the federal complex and industry. Utilizing this information, FETC and DOE can make critical decisions on their own use and implementation of the ISO 14000 standards. The information platform also provides FETC with a forum to present information on its activities, such as the DOE ISO 14000 Pilot Projects, and as an information gathering and dissemination resource for its publications. GETE works with an array of national and international contacts and content sources to gather information and provide daily updates on ISO 14000 and related environmental management issues.

  4. Does the selection of ISO 14001 registrars matter? Registrar reputation and environmental policy statements in China.

    Science.gov (United States)

    Fryxell, Gerald E; Chung, Shan Shan; Lo, Carlos W H

    2004-05-01

    This study investigates the relationship between characteristics of environmental policy statements and the reputations of ISO 14001 registrars who had performed certification audits of firms operating in mainland China. Three characteristics of environmental policy statements were examined: (1) The conformance of the policy to strict interpretations of the international standard; (2) The policy statement's adherence to the good practice guidelines specified in ISO 14004; and, (3) Self-reported evaluations of the policy statement's effectiveness as implemented. Data from 106 facilities in Beijing, Shanghai and Guangzhou reveal that registrar quality has a relatively weak, positive relationship with conformance to both ISO 14001 standards and to ISO 14004 guidelines, but no relationship was observed with the self-reported data. Additional findings are that the use of foreign registrars is significantly associated with the adoption of ISO 14004 guidelines and that conformance with ISO 14001 standards is somewhat higher for international joint ventures and foreign-owned firms than for state-owned enterprises.

  5. Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

    Directory of Open Access Journals (Sweden)

    Bin Fan

    2017-01-01

    Full Text Available Acute energy failure is one of the critical factors contributing to the pathogenic mechanisms of retinal ischemia. Our previous study demonstrated that glucose deprivation can lead to a caspase-dependent cell death of photoreceptors. The aim of this study was to decipher the upstream signal pathway in glucose deprivation- (GD- induced cell death. We mimicked acute energy failure by using glucose deprivation in photoreceptor cells (661W cells. GD-induced oxidative stress was evaluated by measuring ROS with the DCFH-DA assay and HO-1 expression by Western blot analysis. The activation of NOX2/MAPK/NF-κB signal was assessed by Western blot and immunohistochemical assays. The roles of these signals in GD-induced cell death were measured by using their specific inhibitors. Inhibition of Rac-1 and NOX2 suppressed GD-induced oxidative stress and protected photoreceptors against GD-induced cell death. NOX2 was an upstream signal in the caspase-dependent cell death cascade, yet the downstream MAPK pathways were activated and blocking MAPK signals rescued 661W cells from GD-induced death. In addition, GD caused the activation of NF-κB signal and inhibiting NF-κB significantly protected 661W cells. These observations may provide insights for treating retinal ischemic diseases and protecting retinal neurons from ischemia-induced cell death.

  6. A functional tetranucleotide (AAAT) polymorphism in an Alu element in the NF1 gene is associated with mental retardation.

    Science.gov (United States)

    Védrine, Sylviane Marouillat; Vourc'h, Patrick; Tabagh, Refaat; Mignon, Laurence; Höfflin, Saskya; Cherpi-Antar, Catherine; Mbarek, Olivier; Paubel, Agathe; Moraine, Claude; Raynaud, Martine; Andres, Christian R

    2011-03-17

    Mental retardation (MR) is frequent in neurofibromatosis type 1 (NF1). Allele 5 of a tetranucleotide polymorphism in an Alu element (GXAlu) localized in intron 27b of the NF1 gene has previously been associated with autism. We considered that the microsatellite GXAlu could also represent a risk factor in MR without autism. We developed a rapid method for genotyping by non-denaturing HPLC and assayed the allelic variation of GXAlu marker on in vitro gene expression in Cos-7 cells. A French population of 157 individuals (68 non syndromic non familial MR (NS-MR) patients diagnosed in the University Hospital of Tours; 89 controls) was tested in a case-control assay. We observed a significant association (χ(2)=7.96; p=0.005) between alu4 carriers (7 AAAT repeats) and MR (OR: 7.86; 95% C.I.: 2.13-28.9). The relative in vitro expression of a reporter gene encoding chloramphenicol acetyl transferase (CAT) was higher for alu4 and alu5, suggesting a regulation effect for these alleles on gene expression in vivo. Our results showed an association with a polymorphism regulating the NF1 gene or other genes during brain development. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  7. IKK- and NF-κB-mediated functions in carcinogenesis.

    Science.gov (United States)

    Arkan, Melek C; Greten, Florian R

    2011-01-01

    Since the initial cloning of RelA and its close relationship to c-Rel, the cellular homolog of the viral oncoprotein v-Rel, the nuclear factor κB (NF-κB) signaling pathway and its upstream activating kinase complex (IκB-kinase) have been suspected to play a major role in tumorigenesis. This was further corroborated by the discovery of oncogenic mutations in NF-κB proteins in certain lymphoid malignancies and the notion that NF-κB is persistently activated in a large variety of solid tumors. With the advent of conditional knockout mice allowing tissue-specific targeting of the various components of the NF-κB signaling pathway, it was possible to genetically test the cell autonomous and non-autonomous functions of NF-κB in inflammation-associated cancer as well as sporadic cancers. Here, we review molecular evidence that demonstrates the various functions of NF-κB during different tumor stages and that supports the rationale to target NF-κB in cancer prevention and therapy.

  8. OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

    KAUST Repository

    Kim, SoonKap

    2015-11-05

    OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes ( Ehd1, Hd3a , and RFT1 ) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

  9. OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice.

    Science.gov (United States)

    Kim, Soon-Kap; Park, Hyo-Young; Jang, Yun Hee; Lee, Keh Chien; Chung, Young Soo; Lee, Jeong Hwan; Kim, Jeong-Kook

    2016-03-01

    OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response through the modulation of three flowering-time genes ( Ehd1, Hd3a , and RFT1 ) in rice. Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11). Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

  10. Evaluation of the Petrifilm™ EB and TEMPO(®) EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk.

    Science.gov (United States)

    Cirolini, Andréia; Miotto, Marília; Machado, Fernanda Morgana; da Silva, Helen Silvestre; Ogliari, Paulo José; Vieira, Cleide Rosana Werneck

    2013-01-01

    The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the Petrifilm™ EB and the TEMPO(®) EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method Petrifilm™ EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the Petrifilm™ EB system and the TEMPO(®) EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.

  11. Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk

    Directory of Open Access Journals (Sweden)

    Andréia Cirolini

    2013-09-01

    Full Text Available The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of pasteurized milk, r = 0.98 for artificially contaminated pasteurized milk and r = 0.99 for the artificially contaminated UHT milk. In evaluating the system TEMPO EB ® method and ISO 21528:2 correlation was also significant in the analyzed samples, with r = 0.86 for the microflora of pasteurized milk, r = 0.96 for artificially contaminated pasteurized milk and r = 0.99 for artificially contaminated UHT milk. No statistically significant differences were observed between the three methods conducted to analyze artificially contaminated pasteurized and UHT milk at three inoculum levels. In conclusion, the PetrifilmTM EB system and the TEMPO® EB system may be an alternative to the ISO 21528-2:2004 for the Enterobacteriaceae assay for milk as because of the ease-of-operation and the time reduction achieved for conducting the microbiological assay using these systems.

  12. ISO 9001: 2008 Quality Assurance Assessment of Defense Acquisition University Processes

    Science.gov (United States)

    2012-09-27

    Report No. DoDIG-2012-143 September 27, 2012 ISO 9001 :2008 Quality Assurance Assessment of Defense Acquisition University...DATE 27 SEP 2012 2. REPORT TYPE 3. DATES COVERED 00-00-2012 to 00-00-2012 4. TITLE AND SUBTITLE ISO 9001 :2008 Quality Assurance Assessment of...ACQUISITION UNIVERSITY SUBJECT: ISO 9001 :2008 Quality Assurance Assessment of Defense Acquisition University Processes (Report No. DODIG-2012-143) The

  13. [Approval of ISO/IEC 17025 and quality control of laboratory testing].

    Science.gov (United States)

    Yamamoto, Shigeki; Asakura, Hiroshi; Machii, Kenji; Igimi, Shizunobu

    2010-01-01

    First section of Division of Biomedical Food Research, National Institute of Health Sciences (NIHS) was approved by ISO/IEC 17025 as a laboratory having an appropriate laboratory testing technique. NIHS is the first national laboratory approved by ISO/IEC 17025. NIHS has also been accepted the appropriate technique and facility for the BSL3 level pathogens by ISO/IEC 17025. NIHS is necessary to take an external audit almost every year. This approval is renewed every 4 years.

  14. Implementation of ISO 9000 in the healthcare sector: a case study.

    Science.gov (United States)

    Motwani, J G; Cheng, C H; Madan, M S

    1996-01-01

    In order to compete at the international level, companies are beginning to recognize ISO 9000 registration as a virtual necessity. As a result, the number of ISO certifications issued in the U.S. and Canada tripled between 1992 and 1994. By means of a case study, this paper describes how a large healthcare manufacturing organization recently achieved its ISO 9001 certification. Specifically, the strategies and the process used by the organization in obtaining the registration is explained.

  15. Effects of ISO 9001 on Non-Financial and Financial Performance in the Organizations: A Review

    OpenAIRE

    Neyestani, Behnam

    2017-01-01

    The main purpose of this paper was to specify the impact of ISO 9001 on financial and non-financial performance. For this aim, a comprehensive literature review was carried out on the scholarly published studies, which investigated empirically the effects of ISO 9001 standard on the organization's performance in different sectors. Subsequently, the ISO 9001 studies identified and selected carefully that were relevant to the objectives and criteria of the study with the aim of achieving the re...

  16. Interim Report on ISO TC 163 Working Group 3. Annual progress report

    Energy Technology Data Exchange (ETDEWEB)

    Fairey, Philip [Florida Solar Energy Center, Cocoa, FL (United States)

    2009-04-02

    This reports cover the initial year efforts of the International Standards Organization (ISO) to develop international standards for rating the energy performance of buildings. The author of this report is a participant in this effort. This report summarizes the activities of the ISO Working Group charged with development of these standards and makes recommendations to the sponsors for future U.S. involvement in this ISO effort.

  17. 21 CFR 184.1165 - n-Butane and iso-butane.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true n-Butane and iso-butane. 184.1165 Section 184.1165 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD... Listing of Specific Substances Affirmed as GRAS § 184.1165 n-Butane and iso-butane. (a) n-Butane and iso...

  18. ISO 17025: practical benefits of implementing a quality system.

    Science.gov (United States)

    Honsa, Julie D; McIntyre, Deborah A

    2003-01-01

    As a laboratory certified to ISO 9001:2000 and accredited to ISO 17025, rtech laboratories has incorporated an overall system for technical and quality management, which results in benefits observed in daily laboratory practices. Technical requirements were updated to include the addition of formal personnel training plans and detailed records, method development and validation procedures, measurement of method uncertainty, and a defined equipment calibration and maintenance program. In addition, a stronger definition of the sample preparation process was documented to maintain consistency in sampling, and a more rigorous quality control monitoring program was implemented for chemistry and microbiology. Management quality improvements focused on document control to maintain consistent analytical processes, improved monitoring of supplier performance, a contract review process for documenting customer requirements, and a system for handling customer comments and complaints, with continuous improvement through corrective and preventive action procedures and audits. Quarterly management review of corrective actions, nonconforming testing, and proficiency testing aid in determining long-term trending. The practical benefits of these technical and management quality improvements are seen on a daily basis in the laboratory. Faster identification and resolution of issues regarding methods, personnel or equipment, improved customer satisfaction, meeting quality requirements of specialized customers, and overall increased laboratory business are all the result of implementing an effective quality system.

  19. IMPLEMENTASI MANAJEMEN MUTU ISO 9001: 2000 PADA SMKN 1 KALASAN

    Directory of Open Access Journals (Sweden)

    Jamiatun Jamiatun

    2015-10-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan  implementasi manajemen mutu  ISO 9001: 2000 pada mutu kinerja sekolah yang meliputi komponen input, proses, dan produk serta partisipasi orang tua siswa dalam pelaksanaan program-program sekolah di SMKN 1 Kalasan. Penelitian ini merupakan penelitian studi kasus dengan subjek penelitian  seluruh guru, karyawan, siswa dan orang tua siswa SMKN 1 Kalasan. Sampel dalam penelitian ini adalah guru dan karyawan SMKN 1 Kalasan yang telah mengabdi kurang lebih tiga tahun yang terdiri atas 84 guru dan 29 karyawan, dan orang tua/wali dan siswa yang duduk di kelas XII saat penelitian berlangsung terdiri atas 175 orang tua dan 312 siswa. Teknik analisis data yang digunakan dalam penelitian adalah deskriptif. Data dikumpulkan melalui  kuesioner, wawancara, observasi, dan dokumentasi. Hasil penelitian ini menunjukkan penilaian guru, karyawan, orang tua dan siswa terhadap mutu kinerja komponen input di SMKN 1 Kalasan sangat baik (63,19%, komponen proses sudah baik (50,35%, komponen produk sangat baik (73,61% dan partisipasi orang tua sangat baik (61,14%. Berdasarkan hasil ini, peneliti menyarankan bagi pihak SMKN 1 Kalasan untuk tetap melakukan perbaikan dalam implementasi Sistem Manajemen Mutu ISO 9001: 2000 agar mutu kinerja sekolah dapat ditingkatkan.

  20. An experimental investigation of iso-octane ignition phenomena

    Energy Technology Data Exchange (ETDEWEB)

    Walton, S.M.; He, X.; Zigler, B.T.; Wooldridge, M.S.; Atreya, A. [Department of Mechanical Engineering, University of Michigan, 2350 Hayward St., Ann Arbor, MI 48109-2125 (United States)

    2007-08-15

    High-speed digital imaging has been used in rapid compression facility (RCF) studies to investigate ignition phenomena of iso-octane/air mixtures. Sequential images were captured for each experiment. The results indicate the existence of two ignition regimes. In one domain, ignition is rapid, typically less than 76 {mu}s, and ignition occurs simultaneously throughout the test volume. In the other domain, reaction fronts form and propagate within the test volume prior to volumetric ignition. The data span equivalence ratios from {phi}=0.20 to 1.98, with inert/O{sub 2} gas ratios from 1.38 to 5.89, pressures from 8.7 to 16.6 atm, and temperatures from 903 to 1020 K. The transition between the two regimes is discussed in the context of the mixture composition and experimental conditions. The analysis shows that the fuel mole fraction is a key parameter dictating the boundary between the modes of ignition. Below a critical mole fraction limit, volumetric ignition is observed; above the critical limit, reaction fronts are consistently present prior to volumetric ignition. The ignition delay times for both ignition regimes are well reproduced using a homogeneous simulation with detailed reaction chemistry, when the state conditions are modified to account for the presence of the reaction fronts. The results are discussed in terms of proposed reaction chemistry, ignition theory, and previous studies of iso-octane ignition. (author)

  1. Analisis Pelaksanaan Sertifikasi ISO 9001 : 2008 Puskesmas Kalasan Kabupaten Sleman

    Directory of Open Access Journals (Sweden)

    Khamidah Yuliati

    2016-07-01

    Full Text Available The background of the study was the implementation of standardization of health service quality stated in ISO 9001: 2008 in Kalasan Health Center effectively applied on July 9th, 2009. It was hoped that the implementation of standardization of basic health service quality increase the quality of health service and human resources. The data collection was conducted by interview and questionnaire, and the collected data were in the form of primary and secondary data. The number of the research sample was 40 respondents. The data analysis method used was descriptive. The conclusions of the research result and analysis were (1 all employees increase the service quality for all patients in Kalasan Health Center. The curative aspect was emphasized on outpatients, and the stabilization of emergency and referral patients. (2 Acknowledging qualified work achievements by giving the opportunity to have position promotion as procedure assigned. Work satisfaction was from the existing of formation facility or space condition that gave comfort feeling. (3 Work team and colleague feeling needed to be maintained so that priority gap got decreased. The coordination between the employer and the employee is useful to finish the work. (4 The institution gave guarantee on health service quality which was as the hope of the consumer (patient and strengthened the competitiveness. Therefore, the implementation of quality management system based on the concept of ISO 9001:2008 as the effort to strengthen the competitiveness was urgent.

  2. ISO standards on test methods for water radioactivity monitoring.

    Science.gov (United States)

    Calmet, D; Ameon, R; Bombard, A; Forte, M; Fournier, M; Herranz, M; Jerome, S; Kwakman, P; Llaurado, M; Tokonami, S

    2013-11-01

    Water is vital to humans and each of us needs at least 1.5L of safe water a day to drink. Beginning as long ago as 1958 the World Health Organization (WHO) has published guidelines to help ensure water is safe to drink. Focused from the start on monitoring radionuclides in water, and continually cooperating with WHO, the International Standardization Organization (ISO) has been publishing standards on radioactivity test methods since 1978. As reliable, comparable and 'fit for purpose' results are an essential requirement for any public health decision based on radioactivity measurements, international standards of tested and validated radionuclide test methods are an important tool for production of such measurements. This paper presents the ISO standards already published that could be used as normative references by testing laboratories in charge of radioactivity monitoring of drinking water as well as those currently under drafting and the prospect of standardized fast test methods in response to a nuclear accident. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Field experience in implementing ISO 15189 in Kisumu, Kenya.

    Science.gov (United States)

    Zeh, Clement E; Inzaule, Seth C; Magero, Valentine O; Thomas, Timothy K; Laserson, Kayla F; Hart, Clyde E; Nkengasong, John N

    2010-09-01

    Quality medical laboratory services are an integral part of routine health care, medical research, and public health systems. Despite this vital role, quality laboratory services in Africa are scarce. The crucial need for expanding quality laboratory services throughout sub-Saharan Africa is especially critical because of the region's burden of disease. Fortunately, several plans from supporting international partners are underway to help strengthen laboratory infrastructure in this region. A key component of these initiatives is the enforcement of quality assurance services through accreditation by international standards such as the International Organization for Standardization (ISO) 15189. However, acquisition and maintenance of these standards are a significant challenge, especially in resource-limited settings. The most common limiting factors can include funding, government support, equipment, training opportunities, and poor procurement infrastructure. In this article, we discuss the challenges and benefits accrued in pursuing and sustaining ISO 15189 accreditation for the Kenya Medical Research Institute/Centre for Disease Control HIV-Research Laboratory in Kisumu, Kenya.

  4. Tetrandrine regulates hepatic stellate cell activation via TAK1 and NF-κB signaling.

    Science.gov (United States)

    Li, Xia; Jin, Quan; Wu, Yan-Ling; Sun, Peng; Jiang, Shuang; Zhang, Yu; Zhang, De-Quan; Zhang, Yu-Jing; Lian, Li-Hua; Nan, Ji-Xing

    2016-07-01

    We investigated the anti-fibrotic mechanism of tetrandrine, a bisbenzylisoquinoline alkaloid from the Chinese herb, Stephania tetrandra, on the immortalized HSC-T6 rat hepatic stellate cell line. Tetrandrine (0.39-50μM) dose- and time-dependently inhibited HSC-T6 cell viability within 24h and exhibited almost no cytotoxicity at concentrations lower than 6.25μM in the presence of tumor necrosis factor-α (TNF-α). At a much high concentration (50μM), tetrandrine caused fatal cytotoxity in both HSCs and hepatocytes. TNF-α time-dependently increased α-smooth muscle actin (α-SMA) expression, while a lower concentration of tetrandrine (6.25μM) prior to TNF-α treatment reduced the expression of α-SMA and TNFR-1-associated death domain (TRADD). TNF-α treatment induced TGF-β-activated kinase-1 (TAK1) and c-Jun N-terminal kinase (JNK) phosphorylation, which were attenuated by tetrandrine. Furthermore, TNF-α treatment activated nuclear factor-κB (NF-κB) nuclear translocation and IκB-α degradation. Tetrandrine treatment prior to TNF-α reduced nuclear phosphorylated and total NF-κB p65, while the cytosolic IκB-α and NF-κB p65 levels significantly increased. In addition, treatment with only tetrandrine induced the cleavage of caspase-3 and PARP within a range of higher concentrations. Tetrandrine-induced apoptosis was confirmed by the TUNEL assay and flow-cytometric analysis. Treatment with only tetrandrine markedly reduced α-SMA expression, except for at lower concentrations of tetrandrine. A higher concentration of tetrandrine (25μM) induced a significant increase in JNK and extracellular signal-regulated kinase (ERK) phosphorylation, NF-κB nuclear translocation and IκB-α degradation. In conclusion, the anti-fibrogenic effects of tetrandrine on HSCs involved a dosage-dependent signaling pathway, based on the tetrandrine concentration, by regulating TAK1, JNK and NF-κB. The present data provides strong evidence for the anti-fibrotic dosage

  5. Microbead agglutination based assays

    KAUST Repository

    Kodzius, Rimantas

    2013-01-21

    We report a simple and rapid room temperature assay for point-of-care (POC) testing that is based on specific agglutination. Agglutination tests are based on aggregation of microbeads in the presence of a specific analyte thus enabling the macroscopic observation. Such tests are most often used to explore antibody-antigen reactions. Agglutination has been used for protein assays using a biotin/streptavidin system as well as a hybridization based assay. The agglutination systems are prone to selftermination of the linking analyte, prone to active site saturation and loss of agglomeration at high analyte concentrations. We investigated the molecular target/ligand interaction, explaining the common agglutination problems related to analyte self-termination, linkage of the analyte to the same bead instead of different microbeads. We classified the agglutination process into three kinds of assays: a two- component assay, a three-component assay and a stepped three- component assay. Although we compared these three kinds of assays for recognizing DNA and protein molecules, the assay can be used for virtually any molecule, including ions and metabolites. In total, the optimized assay permits detecting analytes with high sensitivity in a short time, 5 min, at room temperature. Such a system is appropriate for POC testing.

  6. ISO, unique explorer of the invisible cool universe

    Science.gov (United States)

    1995-10-01

    As Roger Bonnet, director of ESA's science programme, explains: "The ISO launch will be the culmination of twelve years of intensive effort to build tile mast powerful and precise infrared space telescope to dote, We fully expect astronomers in Europe. and all over the wand, to be rewarded with unprecedented insights into the particularly rich and fertile sources of the universe hidden in the infrared." For the mission looks set to mark a new step forward for astronomy. To date, infrared astronomy has always been hampered by two major difficulties. The first is that infrared radiation is thermal, calorific. This means that any body which is not absolutely cold naturally emits this type of "heat" or "hidden" radiation, Consequently, at the corresponding wavelengths, all telescopes, detectors and the Earth's atmosphere appear to shine spontaneously, Infrared observation is therefore tantamount to attempting to observe the stars in broad daylight using a telescope a million times more luminous than the stars themselves. Moreover, the atmosphere is partly or totally opaque to infrared light. Hence the great attraction of conducting such observations from space, above the Earth's atmospheric layers. using a very low-temperature telescope. The first - and, so far, truly - Satellite actually to have done so is the Infrared Astronomical Satellite (IRAS) - a joint UK/Dutch/US mission - which surveyed and catalogued the sky far ten months in 1983. A corner of the curtain was thus lifted, The scene was set. Now, the task is to observe each character, each object. And here, infrared astronomy is hampered by a second major difficulty: the very detection of light. For while infrared radiation was discovered back in 1800 (in the solar spectrum). infrared-sensitive photoelectric detectors did not materialise until the Second World War, IRAS itself had to make do without the multi-dimensional set-ups that would have allowed it to record true images. It had to build up its images

  7. A comparison of the ability of DMXAA and xanthenone analogues to activate NF-kappaB in murine and human cell lines.

    Science.gov (United States)

    Woon, See-Tarn; Reddy, Charu B; Drummond, Catherine J; Schooltink, Mary Ann; Baguley, Bruce C; Kieda, Claudine; Ching, Lai-Ming

    2005-01-01

    DMXAA (5,6-dimethylxanthenone-4-acetic acid), the most potent of a series of xanthenone (XAA) analogues developed in this laboratory, is currently undergoing combination clinical trials as an antivascular agent for cancer treatment. XAAs have a complex mode of action, and in vitro assays that are predictive of in vivo antitumor activity have been difficult to develop. In this study, we have utilized a series including XAA, DMXAA, and mono-substituted XAA derivatives to determine firstly whether in vitro NF-kappaB activation of mouse cell lines predicts for the in vivo antitumor potential of this class of agents, and secondly whether the relative activity of these analogues is similar in murine and human cell lines. Electromobility shift assays were used to measure NF-kappaB activation in murine HECPP endothelial and 70Z/3 pre-B cells, and in human HPLNEC.B3 endothelial and Raji B-lymphoma cells. A significant correlation was obtained between NF-kappaB activation in HECPP cells by a series of XAA analogues at 100 microg/ml (r = 0.78, p = 0.008) and at 300 microg/ml (r = 0.75, p = 0.01) and the amount of hemorrhagic necrosis induced in Colon 38 tumors. Different structure-activity relationships were observed in human and murine cell lines. 8-MeXAA, which was inactive in HECPP and 70Z/3 murine cell lines, showed similar NF-kappaB activation to DMXAA in human HPLNEC.B3 cells and Raji B-lymphoma cells. These results suggest that the receptor protein(s) in human cells that mediate the human response may have a lower stringency to that for murine cells. We also noted differences in the dose-response relationships for NF-kappaB activation between lymphoid and endothelial lines that were species independent. With increasing concentrations of DMXAA, NF-kappaB activation in both murine and human lymphoid lines showed a reproducible fluctuation, while in endothelial lines, the intensity of NF-kappaB activation was relatively constant above a threshold concentration. The

  8. Global Impact Estimation of ISO 50001 Energy Management System for Industrial and Service Sectors

    Energy Technology Data Exchange (ETDEWEB)

    Aghajanzadeh, Arian [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Therkelsen, Peter L. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Rao, Prakash [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); McKane, Aimee T. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2016-08-01

    A methodology has been developed to determine the impacts of ISO 50001 Energy Management System (EnMS) at a region or country level. The impacts of ISO 50001 EnMS include energy, CO2 emissions, and cost savings. This internationally recognized and transparent methodology has been embodied in a user friendly Microsoft Excel® based tool called ISO 50001 Impact Estimator Tool (IET 50001). However, the tool inputs are critical in order to get accurate and defensible results. This report is intended to document the data sources used and assumptions made to calculate the global impact of ISO 50001 EnMS.

  9. ISO-66, a novel inhibitor of macrophage migration, shows efficacy in melanoma and colon cancer models.

    Science.gov (United States)

    Ioannou, Kyriaki; Cheng, Kai Fan; Crichlow, Gregg V; Birmpilis, Anastasios I; Lolis, Elias J; Tsitsilonis, Ourania E; Al-Abed, Yousef

    2014-10-01

    Macrophage migration inhibitory factor (MIF) is a pleiotropic pro-inflammatory cytokine, which possesses a contributing role in cancer progression and metastasis and, thus, is now considered a promising anticancer drug target. Many MIF-inactivating strategies have proven successful in delaying cancer growth. Here, we report on the synthesis of ISO-66, a novel, highly stable, small-molecule MIF inhibitor, an analog of ISO-1 with improved characteristics. The MIF:ISO-66 co-crystal structure demonstrated that ISO-66 ligates the tautomerase active site of MIF, which has previously been shown to play an important role in its biological functions. In vitro, ISO-66 enhanced specific and non-specific anticancer immune responses, whereas prolonged administration of ISO-66 in mice with established syngeneic melanoma or colon cancer was non-toxic and resulted in a significant decrease in tumor burden. Subsequent ex vivo analysis of mouse splenocytes revealed that the observed decrease in tumor growth rates was likely mediated by the selective in vivo expansion of antitumor-reactive effector cells induced by ISO-66. Compared to other MIF-inactivating strategies employed in vivo, the anticancer activity of ISO-66 is demonstrated to be of equal or better efficacy. Our findings suggest that targeting MIF, via highly specific and stable compounds, such as ISO-66, may be effective for cancer treatment and stimulation of anticancer immune responses.

  10. The new ISO 6579-1: A real horizontal standard for detection of Salmonella, at last!

    Science.gov (United States)

    Mooijman, Kirsten A

    2018-05-01

    Up to 2016, three international standard methods existed for the detection of Salmonella spp. in food, animal feed and samples from the primary production stage: ISO 6785:2001 for milk and milk products, ISO 6579:2002 for (other) food and animal feed and Annex D of ISO 6579:2007 for samples from the primary production stage. In 2009, an ISO/CEN working group started with the revision of ISO 6579:2002 with two main aims: combining the three aforementioned standards in one document and improving the information in ISO 6579:2002. Additionally it was decided to split ISO 6579 into three parts, where part 1 describes the detection, part 2 the enumeration by mini-MPN (published in 2012) and part 3 the serotyping of Salmonella (published in 2014). This paper describes the experiments and choices made for improving the part on detection of Salmonella (ISO 6579-1). The final voting stage on (draft) ISO 6579-1 was finished by the end of December 2016, with a positive outcome. Finally, a real horizontal standard became available for detection of Salmonella in food, animal feed, environmental samples in the area of food production and food handling and in samples from the primary production stage in 2017. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. [Accreditation of ISO 15189 in the Department of Laboratory Medicine, Kumamoto University Hospital: successful cases].

    Science.gov (United States)

    Obayashi, Konen; Teramoto, Koji; Yamamoto, Keiichi; Ikeda, Katsuyoshi; Ando, Yukio

    2009-02-01

    Recently, attention has been focused on international standard organization (ISO) 15189 accreditation, ensuring the quality and competence of medical laboratories in Japan. The Department of Laboratory Medicine, Kumamoto University Hospital also received ISO 15189 accreditation on August 30, 2007. In this paper, we describe our successful experiences before and after ISO 15189 accreditation, and discuss how to apply the qualification more effectively from now on. The key points to use the ISO 15189 tool effectively were summarized as follows: 1. Making sense of the purpose: Successful leadership is one of the most important factors. Our director came up with our slogan, which was called the 4 S's (speed, service, science, and strictness) to apply ISO 15189. 2. Improvement of technical and scientific competence: the development of detailed standard operating procedures(SOPs) aids the improvement of technical and scientific competence. 3. Enrich the contents of the teaching system: after we received ISO 15189 accreditation, the teaching system, not only for medical students but also medical staff and foreign students, was markedly improved to take advantage of the global standard. As it is expensive to run ISO 15189, we must utilize the specified and/or standard health check ups from now on. A laboratory cafe, which we are preparing in our hospital now, may be a new unique trial of how to apply ISO 15189. In conclusion, ISO 15189 may become an effective tool to develop and advance medical laboratories.

  12. ISO 13485: a complete guide to quality management in the medical device industry

    National Research Council Canada - National Science Library

    Abuhav, Itay

    2012-01-01

    .... Written by an experienced industry professional, this practical book provides a complete guide to the ISO 13485 standard certification for medical device manufacturing in terms of quality control...

  13. ISO/IEC 3850x - Die Normenreihe zur IT-Governance

    OpenAIRE

    Klotz, Michael

    2016-01-01

    In diesem Arbeitspapier wird die Norm "ISO/IEC 38500" beschrieben und analysiert. Die erste Ausgabe der Norm wurde im Juni 2008 als "ISO/IEC 38500:2008 Corporate governance of information technology" veröffentlicht. Die zweite Ausgabe folgte im Februar 2015 als "ISO/IEC 38500:2015 Information technology - Governance of IT for the organization". Im Folgenden wird auf die institutionellen Hintergründe, die wesentlichen Bestandteile der ISO/IEC 38500:2015, das grundlegende Modell und die Prinzip...

  14. Energy management systems in the practice. ISO 50001. A guideline for companies and institutions; Energiemanagementsysteme in der Praxis. ISO 50001. Leitfaden fuer Unternehmen und Organisationen

    Energy Technology Data Exchange (ETDEWEB)

    Kohlenborn, Walter; Kabisch, Sibylle; Klein, Johanna; Richter, Ina; Schuermann, Silas

    2012-06-15

    The guideline under consideration is an updated version of the guideline to the regulation EN 16001 and amended to ISO 50001. This contribution supports institutes in the implementation of an energy management system according to ISO 50001. The differences and commonalities to the European environment management system EMAS as well as to the regulation ISO 14001 are described. EMAS certified companies fulfil regularly all conditions of an energy management system. A practised energy management system is an excellent platform for the implementation of EMAS.

  15. Functional Analysis of Mutations in Exon 9 of NF1 Reveals the Presence of Several Elements Regulating Splicing.

    Directory of Open Access Journals (Sweden)

    Elisabete Hernández-Imaz

    Full Text Available Neurofibromatosis type 1 (NF1 is one of the most common human hereditary disorders, predisposing individuals to the development of benign and malignant tumors in the nervous system, as well as other clinical manifestations. NF1 is caused by heterozygous mutations in the NF1 gene and around 25% of the pathogenic changes affect pre-mRNA splicing. Since the molecular mechanisms affected by these mutations are poorly understood, we have analyzed the splicing mutations identified in exon 9 of NF1, which is particularly prone to such changes, to better define the possible splicing regulatory elements. Using a minigene approach, we studied the effect of five splicing mutations in this exon described in patients. These highlighted three regulatory motifs within the exon. An in vivo splicing analysis of an extensive collection of changes generated in the minigene demonstrated that the CG motif at c.910-911 is critical for the recognition of exon 9. We also found that the GC motif at c.945-946 is involved in exon recognition through SRSF2 and that this motif is part of a Composite Exon Splicing Regulatory Element made up of physically overlapping enhancer and silencer elements. Finally, through an in vivo splicing analysis and in vitro binding assays, we demonstrated that the c.1007G>A mutation creates an Exonic Splicing Silencer element that binds the hnRNPA1 protein. The complexity of the splicing regulatory elements present in exon 9 is most likely responsible for the fact that mutations in this region represent 25% of all exonic changes that affect splicing in the NF1 gene.

  16. Bacterial endotoxin enhances colorectal cancer cell adhesion and invasion through TLR-4 and NF-kappaB-dependent activation of the urokinase plasminogen activator system.

    LENUS (Irish Health Repository)

    Killeen, S D

    2009-05-19

    Perioperative exposure to lipopolysaccharide (LPS) is associated with accelerated metastatic colorectal tumour growth. LPS directly affects cells through Toll-like receptor 4 (TLR-4) and the transcription factor NF-kappaB. The urokinase plasminogen activator (u-PA) system is intimately implicated in tumour cell extracellular matrix (ECM) interactions fundamental to tumour progression. Thus we sought to determine if LPS directly induces accelerated tumour cell ECM adhesion and invasion through activation of the u-PA system and to elucidate the cellular pathways involved. Human colorectal tumour cell lines were stimulated with LPS. u-PA concentration, u-PA activity, active u-PA, surface urokinase plasminogen activator receptor (u-PAR) and TLR-4 expression were assessed by ELISA, colorimetric assay, western blot analysis and flow cytometry respectively. In vitro tumour cell vitronectin adhesion and ECM invasion were analysed by vitronectin adhesion assay and ECM invasion chambers. u-PA and u-PAR function was inhibited with anti u-PA antibodies or the selective u-PA inhibitors amiloride or WXC-340, TLR-4 by TLR-4-blocking antibodies and NF-kappaB by the selective NF-kappaB inhibitor SN-50. LPS upregulates u-PA and u-PAR in a dose-dependent manner, enhancing in vitro tumour cell vitronectin adhesion and ECM invasion by >40% (P<0.01). These effects were ameliorated by u-PA and u-PAR inhibition. LPS activates NF-kappaB through TLR-4. TLR-4 and NF-kappaB inhibition ameliorated LPS-enhanced u-PA and u-PAR expression, tumour cell vitronectin adhesion and ECM invasion. LPS promotes tumour cell ECM adhesion and invasion through activation of the u-PA system in a TLR-4- and NF-kappaB-dependent manner.

  17. Modification of Cysteine Residue in p65 Subunit of Nuclear Factor-κB by Picroliv Suppresses NF-κB-Regulated Gene Products and Potentiates Apoptosis

    Science.gov (United States)

    Anand, Preetha; Kunnumakkara, Ajaikumar B.; Harikumar, Kuzhuvelil B.; Ahn, Kwang S.; Badmaev, Vladimir

    2009-01-01

    Picroliv, an iridoid glycoside derived from the plant Picrorhiza kurroa, is used traditionally to treat fever, asthma, hepatitis and other inflammatory conditions. However, the exact mechanism of its therapeutic action is still unknown. Because NF-κB activation plays a major role in inflammation and carcinogenesis, we postulated that picroliv must interfere with this pathway by inhibiting the activation of NF-κB mediated signal cascade. Electrophoretic mobility shift assay showed that pretreatment with picroliv abrogated TNF induced activation of NF-κB. The glycoside also inhibited NF-κB activated by carcinogenic and inflammatory agents such as CSC, PMA, OA, H2O2, LPS and EGF. When examined for the mechanism of action, we found that picroliv inhibited activation of IκBα kinase, leading to inhibition of phosphorylation and degradation of IκBα. It also inhibited phosphorylation and nuclear translocation of p65. Further studies revealed that picroliv directly inhibits the binding of p65 to DNA, which was reversed by the treatment with reducing agents, suggesting a role for a cysteine residue in interaction with picroliv. Mutation of cysteine residue 38 in p65 to serine abolished this effect of picroliv. NF-κB inhibition by picroliv lead to suppression of NF-κB-regulated proteins, including those linked with cell survival (IAP1, Bcl-2, Bcl-xL, survivin, and TRAF2), proliferation (cyclin D1 and COX-2), angiogenesis (VEGF), and invasion (ICAM-1 and MMP-9). Suppression of these proteins, enhanced apoptosis induced by TNF. Overall, our results demonstrate that picroliv inhibits the NF-κB activation pathway, which may explain its anti-inflammatory and anticarcinogenic effects. PMID:18974130

  18. NRP/Optineurin Cooperates with TAX1BP1 to potentiate the activation of NF-kappaB by human T-lymphotropic virus type 1 tax protein.

    Directory of Open Access Journals (Sweden)

    Chloé Journo

    2009-07-01

    Full Text Available Nuclear factor (NF-kappaB is a major survival pathway engaged by the Human T-Lymphotropic Virus type 1 (HTLV-1 Tax protein. Tax1 activation of NF-kappaB occurs predominantly in the cytoplasm, where Tax1 binds NF-kappaB Essential Modulator (NEMO/IKKgamma and triggers the activation of IkappaB kinases. Several independent studies have shown that Tax1-mediated NF-kappaB activation is dependent on Tax1 ubiquitination. Here, we identify by co-immunoprecipitation assays NEMO-Related Protein (NRP/Optineurin as a binding partner for Tax1 in HTLV-1 infected and Tax1/NRP co-expressing cells. Immunofluorescence studies reveal that Tax1, NRP and NEMO colocalize in Golgi-associated structures. The interaction between Tax1 and NRP requires the ubiquitin-binding activity of NRP and the ubiquitination sites of Tax1. In addition, we observe that NRP increases the ubiquitination of Tax1 along with Tax1-dependent NF-kappaB signaling. Surprisingly, we find that in addition to Tax1, NRP interacts cooperatively with the Tax1 binding protein TAX1BP1, and that NRP and TAX1BP1 cooperate to modulate Tax1 ubiquitination and NF-kappaB activation. Our data strongly suggest for the first time that NRP is a critical adaptor that regulates the assembly of TAX1BP1 and post-translationally modified forms of Tax1, leading to sustained NF-kappaB activation.

  19. Point mutation in the NF2 gene of HEI-193 human schwannoma cells results in the expression of a merlin isoform with attenuated growth suppressive activity

    Energy Technology Data Exchange (ETDEWEB)

    Lepont, Pierig; Stickney, John T.; Foster, Lauren A.; Meng, Jin-Jun; Hennigan, Robert F. [Department of Cell and Cancer Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States); Ip, Wallace [Department of Cell and Cancer Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267-0521 (United States)], E-mail: wallace.ip@uc.edu

    2008-01-01

    Neurofibromatosis type 2 (NF2) is a genetic disorder characterized by the formation of bilateral schwannomas of the eighth cranial nerve. Although the protein product of the NF2 gene (merlin) is a classical tumor suppressor, the mechanism by which merlin suppresses cell proliferation is not fully understood. The availability of isolated tumor cells would facilitate a better understanding of the molecular function of merlin, but primary schwannoma cells obtained from patients grow slowly and do not yield adequate numbers for biochemical analysis. In this study, we have examined the NF2 mutation in HEI-193 cells, an immortalized cell line derived from the schwannoma of an NF2 patient. Previous work showed that the NF2 mutation in HEI-193 cells causes a splicing defect in the NF2 transcript. We have confirmed this result and further identified the resultant protein product as an isoform of merlin previously designated as isoform 3. The level of isoform 3 proteins in HEI-193 cells is comparable to the levels of merlin isoforms 1 and 2 in normal human Schwann cells and several other immortalized cell lines. In contrast to many mutant forms of merlin, isoform 3 is as resistant to proteasomal degradation as isoforms 1 and 2 and can interact with each of these isoforms in vivo. Cell proliferation assays showed that, in NF2{sup -/-} mouse embryonic fibroblasts, exogenously expressed merlin isoform 3 does exhibit growth suppressive activity although it is significantly lower than that of identically expressed merlin isoform 1. These results indicate that, although HEI-193 cells have undetectable levels of merlin isoforms 1 and 2, they are, in fact, not a merlin-null model because they express the moderately active growth suppressive merlin isoform 3.

  20. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K [Pleasanton, CA; Snyderman, Neal J [Berkeley, CA; Rowland, Mark S [Alamo, CA

    2012-05-15

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  1. Absolute nuclear material assay

    Science.gov (United States)

    Prasad, Manoj K [Pleasanton, CA; Snyderman, Neal J [Berkeley, CA; Rowland, Mark S [Alamo, CA

    2010-07-13

    A method of absolute nuclear material assay of an unknown source comprising counting neutrons from the unknown source and providing an absolute nuclear material assay utilizing a model to optimally compare to the measured count distributions. In one embodiment, the step of providing an absolute nuclear material assay comprises utilizing a random sampling of analytically computed fission chain distributions to generate a continuous time-evolving sequence of event-counts by spreading the fission chain distribution in time.

  2. IT内部統制に関する考察 : ISO27001 ISO9001の活用

    OpenAIRE

    赤林, 隆仁; Takahito, AKABAYASHI

    2007-01-01

    日本の上場企業はIT化が進展しており、内部統制においてもIT を前提としたものが要求される。そのための安価で適用し易いフレームワークとして多くの企業で経験のあるISO 27001(情報セキュリティシステム)、ISO 9001(品質システム)を活用できないかを検討した。その結果この2 つを併用するとIT を前提とする内部統制の要求事項を網羅できることが判明した。内部統制を意識して2 つの基準を適用すれば内部統制に係わる費用の削減につながることが推測された。...

  3. Data of evolutionary structure change: 1AOGA-2J3NF [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available EVID>EVID> 7 2J3N F 2J3NF...EVID>EVID> 8 2J3N F 2J3NF...1AOGA-2J3NF 1AOG 2J3N A F -SKIFDLVVIGAGSGGLEAAWNAATLYKKRVAVIDVQMV... HHHHEEEEE HHHH EEEEEEEEE EEEEEEEEE HHHHHHHHHHHH HHHHH GGG ---- EVID>...hain> 2J3N F 2J3NF

  4. GeNF - Experimental report 2007

    Energy Technology Data Exchange (ETDEWEB)

    Pranzas, P.K.; Schreyer, A.; Willumeit, R. (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. fuer Materialforschung

    2008-11-05

    At the Geesthacht Neutron Facility GeNF about 203 experiments were performed in 2007 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guests and by the GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 70 contributions in the present annual experimental report for the year 2007. The contributions may contain one or also several combined experiments. During 2007 the GKSS research reactor FRG-1 achieved an operation time of 204 days at the full 5 MW reactor power providing a neutron flux of ca. 1.4 x 10{sup 14} thermal neutrons/cm{sup 2}s. In May/June 2007 the FRG-1 was upgraded with a new cold neutron source yielding a flux increase at the five instruments using cold neutrons of up to 40 %. The focus of the in house R and D work at GeNF instruments in 2007 was the characterisation of nano-structures in engineering materials, the analysis of stresses and textures in welds and technical structures at SANS-2, DCD, ARES-2 and TEX-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at NeRo, PNR, POLDI and ROeDI. The modern experiment control hardware and software based on LabView was continuously improved on all instruments. In the appendices I and II the experimental reports of the GKSS outstation at the FRM II are attached as well as of the GKSS outstation at DESY. At the neutron reflectometer REFSANS at FRM II measurements are possible using a broad range of the scattering vector with reflectivities up to 10{sup -7}. Three reports show the activities of GKSS in the field of texture measurement at the instrument STRESS-SPEC. The instrument HARWI II at DESY is accepted very well by the community and is overbooked in all fields

  5. GeNF - Experimental report 2008

    Energy Technology Data Exchange (ETDEWEB)

    Pranzas, Philipp Klaus; Mueller, Martin; Willumeit, Regine; Schreyer, Andreas (eds.) [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. fuer Materialforschung

    2009-12-11

    At the Geesthacht Neutron Facility GeNF about 182 experiments were performed in 2008 by GKSS and by or for external users, partners or contractors. In most cases the measurements were performed and analysed in cooperation by the guests, by GKSS staff or by the permanent external user group staff. The activities, which are based on a proposal procedure and on the in house R and D program, are reported in 76 contributions in the present annual experimental report for the year 2008. The contributions may contain several combined experiments. During 2008 the GKSS research reactor FRG-1 achieved an operation time of 175 days at the full 5 MW reactor power providing a neutron flux of ca. 1.4.10{sup 14} thermal neutrons/cm{sup 2} s. The focus of the in house R and D work at GeNF instruments in 2008 was the characterisation of nanostructures in engineering materials, the analysis of stresses and textures in welds and technical structures at SANS-2, DCD, ARES-2 and TEX-2, the structural investigation of hydrogen containing substances such as polymers, colloids and biological macromolecules at SANS-1 as well as the characterisation of magnetic thin films at NeRo, PNR, POLDI and ROeDI. The modern experiment control hardware (e.g. sample environments, like magnets, cryostats or furnaces) and software based on LabView was continuously improved on all instruments. In the appendices I and II the experimental reports of the GKSS outstation at the FRM II are attached as well as of the GKSS outstation at DESY. The massive activity at the FRM II outstation is documented by the increasing number of REFSANS reports, accumulated to nine. Three reports show the activities of GKSS in the field of texture measurement at the instrument STRESS-SPEC. The instrument HARWI II at the synchrotron storage ring DORIS III at DESY is accepted very well by the community and is heavily overbooked in all fields (tomography, diffraction, etc.). After an 8-month shutdown period for an upgrade in the frame

  6. The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status

    Directory of Open Access Journals (Sweden)

    Xiaobin Zeng

    2014-06-01

    Full Text Available Wampee (Clausena lansium fruits (CLS, whose pulp can be used to prepare fruit cups, desserts, jam, or jelly, can be eaten along with the peel. In this study, a PC12 cell model was built to observe the protective effect of CLS against H2O2-induced oxidative stress. We found that pretreatment with CLS increased cell viability and inhibited cytotoxicity, caspase-3 activity and DNA condensation. CLS also attenuated the increase in ROS production and MMP reduction. Moreover, we attempted to determine whether CLS suppressed the expression and phosphorylation of NF-κB. Western blot and immunostaining assay revealed that CLS inhibited H2O2-induced up-regulation of NF-κB p65 and pNF-κB p65. And CLS significantly suppressed the translocation of NF-κB p65 and pNF-κB p65 from cytoplasm to nuclear. Also, seven major compounds including a new flavanoid, luteolin-4'-O-β-d-gluco-pyranoside (3 and six known compounds 1,2, 4–7 were isolated and identified from CLS. Their antioxidative and H2O2-induced PC12 cell apoptosis-reversing activity were determined. These findings suggest that CLS and its major constituents (flavanoids may be potential antioxidant agents and should encourage further research into their use as a functional food for neurodegenerative diseases.

  7. Identification of a potent natural triterpenoid inhibitor of proteosome chymotrypsin-like activity and NF-κB with antimyeloma activity in vitro and in vivo

    Science.gov (United States)

    Schmidt, Jessica; Keats, Jonathan J.; Shi, Chang-Xin; Zhu, Yuan Xiao; Palmer, Stephen E.; Mao, Xinliang; Schimmer, Aaron D.; Stewart, A. Keith

    2009-01-01

    As multiple myeloma tumors universally dysregulate cyclin D genes we conducted high-throughput chemical library screens for compounds that induce suppression of cyclin D2 promoter transcription. The top-ranked compound was a natural triterpenoid, pristimerin. Strikingly, the early transcriptional response of cells treated with pristimerin closely resembles cellular responses elicited by proteosome inhibitors, with rapid induction of heat shock proteins, activating transcription factor 3 (ATF3), and CHOP. Enzymatic assays and immunoblotting confirm that pristimerin rapidly (triterpenoids including pristimerin inhibit NF-κB activation via inhibition of IKKα or IKKβ, whereas proteosome inhibitors instead suppress NF-κB function by impairing degradation of ubiquitinated IκB. By inhibiting both IKK and the proteosome, pristimerin causes overt suppression of constitutive NF-κB activity in myeloma cells that may mediate its suppression of cyclin D. Multiple myeloma is exquisitely sensitive to proteosome or NF-κB pathway inhibition. Consistent with this, pristimerin is potently and selectively lethal to primary myeloma cells (IC50 triterpenoid dual-function proteosome/NF-κB inhibitors as therapeutics for human multiple myeloma and related malignancies. PMID:19096011

  8. A NF-κB-dependent dual promoter-enhancer initiates the lipopolysaccharide-mediated transcriptional activation of the chicken lysozyme in macrophages.

    Directory of Open Access Journals (Sweden)

    James Witham

    Full Text Available The transcriptional activation of the chicken lysozyme gene (cLys by lipopolysaccharide (LPS in macrophages is dependent on transcription of a LPS-Inducible Non-Coding RNA (LINoCR triggering eviction of the CCCTC-binding factor (CTCF from a negative regulatory element upstream of the lysozyme transcription start site. LINoCR is transcribed from a promoter originally characterized as a hormone response enhancer in the oviduct. Herein, we report the characterization of this cis-regulatory element (CRE. In activated macrophages, a 60 bp region bound by NF-κB, AP1 and C/EBPβ controls this CRE, which is strictly dependent on NF-κB binding for its activity in luciferase assays. Moreover, the serine/threonine kinase IKKα, known to be recruited by NF-κB to NF-κB-dependent genes is found at the CRE and within the transcribing regions of both cLys and LINoCR. Such repartition suggests a simultaneous promoter and enhancer activity of this CRE, initiating cLys transcriptional activation and driving CTCF eviction. This recruitment was transient despite persistence of both cLys transcription and NF-κB binding to the CRE. Finally, comparing cLys with other LPS-inducible genes indicates that IKKα detection within transcribing regions can be correlated with the presence of the elongating form of RNA polymerase II or concentrated in the 3' end of the gene.

  9. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-κB1

    Directory of Open Access Journals (Sweden)

    Peiquan Li

    2016-05-01

    Full Text Available Aims: Aberrant expression of microRNA-340 (miR-340 has been frequently reported in some cancers excluding ovarian cancer (OC. The role and its molecular mechanism of miR-340 in OC have not been reported. Methods: Real-time PCR was performed to detect the expression of miR-340 in OC cell lines. MiR-340 mimic and negative control were transfected into OC cells and the effects of miR-340 on the cell proliferation, cell cycle, apoptosis and metastasis were investigated by Brdu-ELISA assay, flow cytometry, qRT-PCR, Transwell and ELISA assays. Furthermore, protein level of NF-κB1 was measured by Western blotting. Meanwhile, luciferase assays were performed to validate NF-κB1 as miR-340 target in OC cells. Results: In this study, we explored the effects of miR-340 overexpression on apoptosis, invasion and EMT in OC cells. The mRNA level of miR-340 in OC cell lines and tissues was evidently reduced. The miR-340 mimic was transiently transfected into OC cells using Lipofectamine™ 2000 reagent. Subsequently, the Brdu-ELISA results showed that introduction of miR-340 inhibited cell proliferation. Our data also demonstrated that miR-340 mimic arrested cell cycle progression and promoted apoptosis of OC cells. In addition, miR-340 overexpression could also inhibit invasion and EMT of OC cells. qRT-PCR were used to determined the expressions of matrix metalloproteinase-2 and -9 (MMP-2 and -9 in OC cells. Next, we found that NF-κB1 expression was evidently reduced by up-regulation of miR-340. Bioinformatics analysis predicted that the NF-κB1 was a potential target gene of miR-340. Luciferase reporter assay further confirmed that miR-340 could directly target the 3' UTR of NF-κB1. Moreover, overexpression of NF-κB1 in OC cells transfected with miR-340 mimic partially reversed the inhibitory of miR-340 mimic. Conclusion: miR-340 induced cell apoptosis and inhibited metastasis in OC cells by down-regulation of NF-κB1.

  10. Osthole inhibits the invasive ability of human lung adenocarcinoma cells via suppression of NF-κB-mediated matrix metalloproteinase-9 expression

    Energy Technology Data Exchange (ETDEWEB)

    Kao, Shang-Jyh [Department of Chest Medicine, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan (China); School of Respiratory Therapy, Taipei Medical University, Taipei Taiwan (China); Su, Jen-Liang [Graduate Institute of Cancer Biology, College of Medicine, China Medical University, Taichung, Taiwan (China); Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan (China); Department of Biotechnology, Asia University, Taichung, Taiwan (China); Chen, Chi-Kuan [Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China); Yu, Ming-Chih; Bai, Kuan-Jen; Chang, Jer-Hua [Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan (China); Bien, Mauo-Ying [School of Respiratory Therapy, Taipei Medical University, Taipei Taiwan (China); Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan (China); Yang, Shun-Fa [Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan (China); Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan (China); Chien, Ming-Hsien, E-mail: mhchien1976@gmail.com [Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan (China)

    2012-05-15

    The induction of matrix metalloproteinase (MMP)-9 is particularly important for the invasiveness of various cancer cells. Osthole, a natural coumarin derivative extracted from traditional Chinese medicines, is known to inhibit the proliferation of a variety of tumor cells, but the effect of osthole on the invasiveness of tumor cells is largely unknown. This study determines whether and by what mechanism osthole inhibits invasion in CL1-5 human lung adenocarcinoma cells. Herein, we found that osthole effectively inhibited the migratory and invasive abilities of CL1-5 cells. A zymographic assay showed that osthole inhibited the proteolytic activity of MMP-9 in CL1-5 cells. Inhibition of migration, invasion, and MMP2 and/or MMP-9 proteolytic activities was also observed in other lung adenocarcinoma cell lines (H1299 and A549). We further found that osthole inhibited MMP-9 expression at the messenger RNA and protein levels. Moreover, a chromatin immunoprecipitation assay showed that osthole inhibited the transcriptional activity of MMP-9 by suppressing the DNA binding activity of nuclear factor (NF)-κB in the MMP-9 promoter. Using reporter assays with point-mutated promoter constructs further confirmed that the inhibitory effect of osthole requires an NF-κB binding site on the MMP-9 promoter. Western blot and immunofluorescence assays demonstrated that osthole inhibited NF-κB activity by inhibiting IκB-α degradation and NF-κB p65 nuclear translocation. In conclusion, we demonstrated that osthole inhibits NF-κB-mediated MMP-9 expression, resulting in suppression of lung cancer cell invasion and migration, and osthole might be a potential agent for preventing the invasion and metastasis of lung cancer. -- Highlights: ► Osthole treatment inhibits lung adenocarcinoma cells migration and invasion. ► Osthole reduces the expression and proteolytic activity of MMP-9. ► Osthole inhibits MMP-9 transcription via suppression of NF-κB binding activity. ► Osthole

  11. Nuclear Factor-κB (NF-κB Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1 Gene

    Directory of Open Access Journals (Sweden)

    Yo-Wen Liu

    2012-12-01

    Full Text Available The human Leucine-rich Repeats and WD repeat Domain containing 1 (LRWD1 gene was originally identified by cDNA microarray as one of the genes down-regulated in the testicular tissues of patients with severe spermatogenic defects. Human LRWD1 is a testicular-enriched protein that is present predominantly in the cytoplasm of spermatocytes and spermatids and colocalizes with the centrosome at the base of sperm tail. Reporter assay, Chromatin immunoprecipitation (ChIP analysis, and gel electrophoretic mobility shift assay (EMSA were used to identify the core promoter region of LRWD1. A 198 bp segment upstream of the LRWD1 transcription initiation site exhibited promoter activity. The LRWD1 core promoter lacked a TATA box but contained a NF-κB binding site. Chromatin immunoprecipitation (ChIP analysis and gel electrophoretic mobility shift assay (EMSA showed basal binding of the NF-κB subunit to the LRWD1 promoter. LRWD1 promoter activity was positively regulated by NF-κB, and this regulation was dependent on the presence of the conserved κB site in the LRWD1 promoter region. Our data suggest that NF-κB is an important regulator for the expression of LRWD1. This is the first study showing that the expression of the testis-enriched LRWD1 gene is regulated by NF-κB.

  12. Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies

    Directory of Open Access Journals (Sweden)

    Listwak Samuel J

    2011-10-01

    Full Text Available Abstract Background The characterization and cellular localization of transcription factors like NF-κB requires the use of antibodies for western blots and immunohistochemistry. However, if target protein levels are low and the antibodies not well characterized, false positive data can result. In studies of NF-κB activity in the CNS, antibodies detecting NF-κB proteins have been used to support the finding that NF-κB is constitutively active in neurons, and activity levels are further increased by neurotoxic treatments, glutamate stimulation, or elevated synaptic activity. The specificity of the antibodies used was analyzed in this study. Methods Selectivity and nonselectivity of commonly used commercial and non-commercial p50 and p65 antibodies were demonstrated in western blot assays conducted in tissues from mutant gene knockout mice lacking the target proteins. Results A few antibodies for p50 and p65 each mark a single band at the appropriate molecular weight in gels containing proteins from wildtype tissue, and this band is absent in proteins from knockout tissues. Several antibodies mark proteins that are present in knockout tissues, indicating that they are nonspecific. These include antibodies raised against the peptide sequence containing the nuclear localization signals of p65 (MAB3026; Chemicon and p50 (sc-114; Santa Cruz. Some antibodies that recognize target proteins at the correct molecular weight still fail in western blot analysis because they also mark additional proteins and inconsistently so. We show that the criterion for validation by use of blocking peptides can still fail the test of specificity, as demonstrated for several antibodies raised against p65 phosphorylated at serine 276. Finally, even antibodies that show specificity in western blots produce nonspecific neuronal staining by immunohistochemistry. Conclusions We note that many of the findings in the literature about neuronal NF-κB are based on data garnered

  13. Aberrant Gene Expression in NF1-Mediated Oncogenesis. Addendum

    National Research Council Canada - National Science Library

    Shields, Janiel M

    2004-01-01

    Neurofibromatosis type I (NF1) is a familial multisystem disease that occurs in approximately one in 3,500 people designating it as one of the most common autosomal-dominant human diseases. Approximately 5...

  14. ISO 50001 and SEP Faster and Cheaper - Exploring the Enterprise-Wide Approach

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Jingjing [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Rao, Prakash [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Therkelsen, Peter [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sheaffer, Paul [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Scheihing, Paul [USDOE, Washington, DC (United States); Tamm, Yannick [Energetics, Inc. (United States)

    2017-06-01

    ISO 50001 and other management systems (e.g., ISO 9001 and ISO 14001) allow for implementation and certification at the enterprise level. The "Central Office" concept, which allows a small group of employees to manage and facilitate the organization’s energy management system (EnMS) at the enterprise level, was introduced within the ISO 50003 standard to provide guidance to ISO 50001 certification bodies. Four industrial companies have partnered with the United States Department of Energy to pilot the enterprise-wide ISO 50001/SEP concept under the Better Buildings Superior Energy Performance (SEP) Enterprise-wide Accelerator. Each organization developed a Central Office to host their EnMS while implementing ISO 50001/SEP at multiple physically separated sites. The four corporate partners tailored their Central Office implementation model to meet their own specific circumstances and needs. This paper reviews the commonalities, differences, and benefits of each of these enterprise-wide implementation models, including organizational structures, Central Office staff responsibilities, and key strategies. The cost savings and benefits of using the enterprise-wide approach were assessed, including the cost per site compared with that of a conventional, single-site ISO 50001/SEP implementation approach. This paper also discusses the drivers for the cost reductions realized through these enterprise-wide approaches. The four partner companies worked with 30 total sites. On average, these 30 sites improved energy performance by 5% annually over their SEP achievement periods, saved more than $600,000 annually in energy costs and reduced implementation cost for ISO 50001 and SEP by $19,000 and 0.8 Full Time Equivalent × years (FTE-yr) of staff time per site. The results can inform other organizations seeking to implement enterprise-wide ISO 50001/SEP, as well as energy efficiency organizations seeking to promote wider adoption of ISO 50001 implementation.

  15. The Role of NF1 in Memory Retrieval

    Science.gov (United States)

    2014-09-01

    critical for olfactory memory , didn’t affect LTM, whereas overexpression within octopamine neurons showed a phenotype of enhanced appetitive LTM (Fig... memory .
Our
investigation
over
the
entire
funding
 period
leads
to
following
discoveries.
First,
NF1
is
critical
for
supporting
multiple
types
of
long...term
 memories ,
including
aversive
and
appetitive
classic
conditioning.
Second,
it
regulates
 memory 
 bi‐directionally.
A
loss
of
NF1
functions

  16. Targeting Signaling to YAP for the Therapy of NF2

    Science.gov (United States)

    2016-12-01

    any step of our newly identified pathway, and to test the preclinical efficacy of lead compounds in xenograft models of NF2. During this grant, we have...generated NF2 mutant cell lines expressing two different types of YAP reporters and tested their suitability for high throughput screening. 15...interacts through its coiled-coil segment with the central coiled-coil segment of AMOT proteins, which localize to Adherens Junctions (AJs) and

  17. Exploring Neurofibromin Function in a Yeast Model of NF1

    Science.gov (United States)

    2011-11-01

    to define NF1 disease mechanisms. Budding yeast, Saccharomyces cerevisiae , have two NF1-like genes, called IRA1 and IRA2. In year one of the project...mammalian cells and in Drosophila. References Hohfeld, J., Veenhuis, M., and Kunau, W.H. (1991). PAS3, a Saccharomyces cerevisiae gene...anaerobiosis yes yes PEX 11 Peroxisomal membrane protein required for peroxisome proliferation and medium -chain fatty acid oxidation, most abundant

  18. Management system quality of service radio physics and RR. ISO 9001; Sistema de gestion de la calidad de un servicio de radiofisica y P. R. segun ISO 9001

    Energy Technology Data Exchange (ETDEWEB)

    Gil Agudo, A.; Torres Donaire, J.; Jimenez, J. C.; Carrascosa Fernandez, C.; Arjona Gutierrez, J.

    2011-07-01

    One of the tool increasingly deployed for the optimization of the procedures is the process management system according to a Quality Management. Likewise, for specific areas, such as central services within large institutions such as the General Hospitals of the systems of quality management is the most widely used ISO 9001. We describe in this paper our experience in implementing the system ISO 9001 in our Department of Radio physics and Radiation Protection (SRFPR).

  19. Cancer Cell Growth Inhibitory Effect of Bee Venom via Increase of Death Receptor 3 Expression and Inactivation of NF-kappa B in NSCLC Cells

    Directory of Open Access Journals (Sweden)

    Kyung Eun Choi

    2014-07-01

    Full Text Available Our previous findings have demonstrated that bee venom (BV has anti-cancer activity in several cancer cells. However, the effects of BV on lung cancer cell growth have not been reported. Cell viability was determined with trypan blue uptake, soft agar formation as well as DAPI and TUNEL assay. Cell death related protein expression was determined with Western blotting. An EMSA was used for nuclear factor kappaB (NF-κB activity assay. BV (1–5 μg/mL inhibited growth of lung cancer cells by induction of apoptosis in a dose dependent manner in lung cancer cell lines A549 and NCI-H460. Consistent with apoptotic cell death, expression of DR3 and DR6 was significantly increased. However, deletion of DRs by small interfering RNA significantly reversed BV induced cell growth inhibitory effects. Expression of pro-apoptotic proteins (caspase-3 and Bax was concomitantly increased, but the NF-κB activity and expression of Bcl-2 were inhibited. A combination treatment of tumor necrosis factor (TNF-like weak inducer of apoptosis, TNF-related apoptosis-inducing ligand, docetaxel and cisplatin, with BV synergistically inhibited both A549 and NCI-H460 lung cancer cell growth with further down regulation of NF-κB activity. These results show that BV induces apoptotic cell death in lung cancer cells through the enhancement of DR3 expression and inhibition of NF-κB pathway.

  20. PROPILVEN LABORATORY EVALUATION ACCORDING TO ISO 17025:2005

    Directory of Open Access Journals (Sweden)

    Peggy Fereira R.

    2013-07-01

    Full Text Available This research aims to evaluate the procedures and methods used in the laboratory Propilven accordance with the provisions of ISO 17025:2005. Propilven, a petrochemicals company in Venezuela and in his laboratory determined the quality of the manufactured product (polypropylene, at all stages, so that the application of this technical standard will increase the confidence level of the results. The research was descriptive, with a non-experimental design, field, using as a data collection method, the interview, survey, observation and review of documents and records. Among the results, the standard currently stands, is in the process of standardization in the laboratory, with a degree of implementation of 50%. Additionally, among the recommendations made, are a series of steps to establish a quality management system based on this standard, which apply not only to this company but for any others who wish to demonstrate their competence to carry out tests and / or calibrations.

  1. Business process mapping techniques for ISO 9001 and 14001 certifications

    Energy Technology Data Exchange (ETDEWEB)

    Klement, R.E.; Richardson, G.D.

    1997-11-01

    AlliedSignal Federal Manufacturing and Technologies/Kansas City (FM and T/KC) produces nonnuclear components for nuclear weapons. The company has operated the plant for the US Department of Energy (DOE) since 1949. Throughout the history of the plant, procedures have been written to reflect the nuclear weapons industry best practices, and the facility has built a reputation for producing high quality products. The purpose of this presentation is to demonstrate how Total Quality principles were used at FM and T/KC to document processes for ISO 9001 and 14001 certifications. The information presented to the reader will lead to a better understanding of business administration by aligning procedures to key business processes within a business model; converting functional-based procedures to process-based procedures for total integrated resource management; and assigning ownership, validation, and metrics to procedures/processes, adding value to a company`s profitability.

  2. Metrology in an ISO 15189 accredited medical biology laboratory

    Directory of Open Access Journals (Sweden)

    Guichet C.

    2014-01-01

    Full Text Available All French medical biology laboratories must be accredited according to ISO 15189 for all tests conducted. Metrology is therefore critical and covers a wide variety of areas. This presentation will focus on the metrology manager’s role which is tailored to the medical biology laboratory: human resources in place, methods used, parameters followed, equipment used and strategies implemented when using equipment which is not connected to the International System of Units. It will be illustrated by examples of in vitro and in vivo clinical biochemistry, biological haematology, human toxicology and radiotoxicology. The presentation will cover the exploitation of results of internal controls and interlaboratory comparisons in order to calculate uncertainties and provide doctors with a result along with an interpretation or opinion to ensure optimum patient care. The conclusion will present the steps carried out at the Laboratoire National d’Essai (French National Testing Laboratory to provide medical biology laboratories with certified clinical biology standards.

  3. [ISO 15189, Up-To-Date Information and Prospective View].

    Science.gov (United States)

    Shimoda, Katsuji

    2016-02-01

    "Accreditation Activities for Medical Laboratories in Japan" Audits for transition to ISO 15189:2012 continue to progress. Besides the continual increase of accreditations for medical laboratory testing and pathological examinations, preparations for the addition of physiological testing to the scope of accreditation have finally been completed. As a part of the revision to Japan's Medical Service Act, the external evaluation of medical laboratories is now a requirement to approve clinical trial core hospitals. Accordingly, the importance of third-party accreditation in medical laboratory testing is attracting a growing level of attention. World Accreditation Day 2015 "Accreditation: Supporting the Delivery of Health and Social Care" JAB is being used to make every effort to contribute to this system in order to improve the quality of healthcare in Japan and the health of its citizens.

  4. Inheritance rules for Hierarchical Metadata Based on ISO 19115

    Science.gov (United States)

    Zabala, A.; Masó, J.; Pons, X.

    2012-04-01

    Mainly, ISO19115 has been used to describe metadata for datasets and services. Furthermore, ISO19115 standard (as well as the new draft ISO19115-1) includes a conceptual model that allows to describe metadata at different levels of granularity structured in hierarchical levels, both in aggregated resources such as particularly series, datasets, and also in more disaggregated resources such as types of entities (feature type), types of attributes (attribute type), entities (feature instances) and attributes (attribute instances). In theory, to apply a complete metadata structure to all hierarchical levels of metadata, from the whole series to an individual feature attributes, is possible, but to store all metadata at all levels is completely impractical. An inheritance mechanism is needed to store each metadata and quality information at the optimum hierarchical level and to allow an ease and efficient documentation of metadata in both an Earth observation scenario such as a multi-satellite mission multiband imagery, as well as in a complex vector topographical map that includes several feature types separated in layers (e.g. administrative limits, contour lines, edification polygons, road lines, etc). Moreover, and due to the traditional split of maps in tiles due to map handling at detailed scales or due to the satellite characteristics, each of the previous thematic layers (e.g. 1:5000 roads for a country) or band (Landsat-5 TM cover of the Earth) are tiled on several parts (sheets or scenes respectively). According to hierarchy in ISO 19115, the definition of general metadata can be supplemented by spatially specific metadata that, when required, either inherits or overrides the general case (G.1.3). Annex H of this standard states that only metadata exceptions are defined at lower levels, so it is not necessary to generate the full registry of metadata for each level but to link particular values to the general value that they inherit. Conceptually the metadata

  5. [Significance and future prospects of the ISO15189 accreditation topics on systems of operation in preparation for accreditation].

    Science.gov (United States)

    Ohno, Hiroie

    2010-01-01

    ISO 15189 is a standard for systems of operation to provide medical laboratory services. It is essential that daily operations be performed in accordance with ISO 15189, but acquisition of accreditation under ISO 15189 should not be the goal. Furthermore, since ISO 15189 does not stipulate operational procedures, the optimal method for the individual laboratory should be used. After obtaining ISO 15189 accreditation, the laboratory only has to manage and perform daily operations in accordance with ISO 15189 on the basis of the quality management system that has been established, and the quality of service that the medical laboratory provides will improve accordingly.

  6. NF-kappaB signaling in the aging process.

    Science.gov (United States)

    Salminen, Antero; Kaarniranta, Kai

    2009-07-01

    The aging process represents a progressive decline in cellular and organism function. Explaining the aging process has given rise to a cornucopia for different theories in which the basic difference has been the question whether aging is genetically regulated or an entropic degeneration process. Different screening techniques have revealed that mammalian aging is associated with the activation of NF-kappaB transcription factor system. The NF-kappaB system is an ancient host defense system concerned with immune responses and different external and internal dangers, such as oxidative and genotoxic stress. NF-kappaB signaling is not only the master regulator of inflammatory responses but can also regulate several homeostatic responses such as apoptosis, autophagy, and tissue atrophy. We will describe how chronic activation of NF-kappaB signaling has the capacity to induce the senescent phenotype associated with aging. Interestingly, several longevity genes such as SIRT1, SIRT6, and FoxOs can clearly suppress NF-kappaB signaling and in this way delay the aging process and extend lifespan. It seems that the aging process is an entropic degeneration process driven by NF-kappaB signaling. This process can be regulated by a variety of longevity genes along with a plethora of other factors such as genetic polymorphism, immune and dietary aspects, and environmental insults.

  7. Mechanism of hypoxia-induced NF-kappaB.

    Science.gov (United States)

    Culver, Carolyn; Sundqvist, Anders; Mudie, Sharon; Melvin, Andrew; Xirodimas, Dimitris; Rocha, Sonia

    2010-10-01

    NF-κB activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, IκB kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2)-dependent pathway distinct from that for other common inducers of NF-κB. This process still requires IKK and the IKK kinase TAK1, like that for inflammatory inducers of NF-κB, but the TAK1-associated proteins TAB1 and TAB2 are not essential. IKK complex activation following hypoxia requires Ubc13 but not the recently identified LUBAC (linear ubiquitin chain assembly complex) ubiquitin conjugation system. In contrast to the action of other NF-κB inducers, IKK-mediated phosphorylation of IκBα does not result in its degradation. We show that this results from IκBα sumoylation by Sumo-2/3 on critical lysine residues, normally required for K-48-linked polyubiquitination. Furthermore, inhibition of specific Sumo proteases is sufficient to release RelA from IκBα and activate NF-κB target genes. These results define a novel pathway regulating NF-κB activation, important to its physiological role in human health and disease.

  8. Mechanism of Hypoxia-Induced NF-κB▿ †

    Science.gov (United States)

    Culver, Carolyn; Sundqvist, Anders; Mudie, Sharon; Melvin, Andrew; Xirodimas, Dimitris; Rocha, Sonia

    2010-01-01

    NF-κB activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, IκB kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2)-dependent pathway distinct from that for other common inducers of NF-κB. This process still requires IKK and the IKK kinase TAK1, like that for inflammatory inducers of NF-κB, but the TAK1-associated proteins TAB1 and TAB2 are not essential. IKK complex activation following hypoxia requires Ubc13 but not the recently identified LUBAC (linear ubiquitin chain assembly complex) ubiquitin conjugation system. In contrast to the action of other NF-κB inducers, IKK-mediated phosphorylation of IκBα does not result in its degradation. We show that this results from IκBα sumoylation by Sumo-2/3 on critical lysine residues, normally required for K-48-linked polyubiquitination. Furthermore, inhibition of specific Sumo proteases is sufficient to release RelA from IκBα and activate NF-κB target genes. These results define a novel pathway regulating NF-κB activation, important to its physiological role in human health and disease. PMID:20696840

  9. Effects of Pharmacologic and Genetic Inhibition of Alk on Cognitive Impairments in NF1 Mutant Mice

    Science.gov (United States)

    2016-08-01

    genetic interaction between Alk and Nf1 in mice is similar to the behavioral phenotypes of Alk and NF1 mutations in flies and that pharmacologic or...the predicted improvement in hippocampal-dependent cognitive function using both genetic and pharmacologic inhibition of Alk in heterozygous NF1...phenotypes of Alk and NF1 mutations in flies and that pharmacologic or genetic inhibition of Alk in NF1 mutant mice will attenuate or even rescue

  10. Children with 5′-end NF1 gene mutations are more likely to have glioma

    OpenAIRE

    Anastasaki, Corina; Morris, Stephanie M.; Gao, Feng; Gutmann, David H.

    2017-01-01

    Objective: To ascertain the relationship between the germline NF1 gene mutation and glioma development in patients with neurofibromatosis type 1 (NF1). Methods: The relationship between the type and location of the germline NF1 mutation and the presence of a glioma was analyzed in 37 participants with NF1 from one institution (Washington University School of Medicine [WUSM]) with a clinical diagnosis of NF1. Odds ratios (ORs) were calculated using both unadjusted and weighted analyses of this...

  11. ISO 14000: Origin, Structure, and Potential Barriers to Implementation.

    Science.gov (United States)

    Casto; Ellisen; Trnovec; Kross; Ginter

    1996-04-01

    The ISO 14000 is likely to become the international standard for environmental management. At present, it is an evolving series of individual voluntary standards and guideline reference documents that provide business management with the structure for managing environmental impacts. These encompass environmental management systems, environmental audits, eco-labeling, environmental performance evaluations, life-cycle assessment, and environmental aspects in product standards. The authors present the rationale for the ISO 14000 and the steps in its evolution so far, as well as its present provisions and their implications and its position with regard to regulatory agencies. Particular attention is paid to the consequences of voluntary disclosure and correction of violations. Hanley & Belfus, Inc. Int J Occup Environ Health 1077-3525 2 2 1996 April/June Perspectives on Rural Environmental Health in Central Europe 125 134 EN Tomas Trnovec Burton C. Kross CIREH-Room 352, International Center, University of Iowa, Iowa City, IA 52242, USA. Emil Ginter Life expectancy is about five to seven years less in Central European countries than in comparable countries in Western Europe. Environmental and occupational health risk factors, along with the socioeconomic and political conditions that have prevailed in this region for the past 40 years, are suspected contributing factors to this condition. The initial impression among observers was that environmental pollution by industry was the primary source of contamination leading to human health effects. Current thinking by the authors recognizes that combinations of personal habits, local environmental emissions (home heating), and occupational risk factors are more likely to be influencing the health of this region, particularly in rural areas. A predictive model for standard mortality rates determined that only three potential risk factors were statistically significant: consumption of alcoholic beverages, consumption of citrus

  12. Polydnavirus Ank proteins bind NF-κB homodimers and inhibit processing of Relish.

    Directory of Open Access Journals (Sweden)

    Kavita Bitra

    Full Text Available Recent studies have greatly increased understanding of how the immune system of insects responds to infection, whereas much less is known about how pathogens subvert immune defenses. Key regulators of the insect immune system are Rel proteins that form Nuclear Factor-κB (NF-κB transcription factors, and inhibitor κB (IκB proteins that complex with and regulate NF-κBs. Major mortality agents of insects are parasitoid wasps that carry immunosuppressive polydnaviruses (PDVs. Most PDVs encode ank genes that share features with IκBs, while our own prior studies suggested that two ank family members from Microplitis demolitor bracovirus (MdBV (Ank-H4 and Ank-N5 behave as IκB mimics. However, the binding affinities of these viral mimics for Rel proteins relative to endogenous IκBs remained unclear. Surface plasmon resonance (SPR and co-immunoprecipitation assays showed that the IκB Cactus from Drosophila bound Dif and Dorsal homodimers more strongly than Relish homodimers. Ank-H4 and -N5 bound Dif, Dorsal and Relish homodimers with higher affinity than the IκB domain of Relish (Rel-49, and also bound Relish homodimers more strongly than Cactus. Ank-H4 and -N5 inhibited processing of compound Relish and reduced the expression of several antimicrobial peptide genes regulated by the Imd signaling pathway in Drosophila mbn2 cells. Studies conducted in the natural host Pseudoplusia includens suggested that parasitism by M. demolitor also activates NF-κB signaling and that MdBV inhibits this response. Overall, our data provide the first quantitative measures of insect and viral IκB binding affinities, while also showing that viral mimics disable Relish processing.

  13. Polydnavirus Ank proteins bind NF-κB homodimers and inhibit processing of Relish.

    Science.gov (United States)

    Bitra, Kavita; Suderman, Richard J; Strand, Michael R

    2012-01-01

    Recent studies have greatly increased understanding of how the immune system of insects responds to infection, whereas much less is known about how pathogens subvert immune defenses. Key regulators of the insect immune system are Rel proteins that form Nuclear Factor-κB (NF-κB) transcription factors, and inhibitor κB (IκB) proteins that complex with and regulate NF-κBs. Major mortality agents of insects are parasitoid wasps that carry immunosuppressive polydnaviruses (PDVs). Most PDVs encode ank genes that share features with IκBs, while our own prior studies suggested that two ank family members from Microplitis demolitor bracovirus (MdBV) (Ank-H4 and Ank-N5) behave as IκB mimics. However, the binding affinities of these viral mimics for Rel proteins relative to endogenous IκBs remained unclear. Surface plasmon resonance (SPR) and co-immunoprecipitation assays showed that the IκB Cactus from Drosophila bound Dif and Dorsal homodimers more strongly than Relish homodimers. Ank-H4 and -N5 bound Dif, Dorsal and Relish homodimers with higher affinity than the IκB domain of Relish (Rel-49), and also bound Relish homodimers more strongly than Cactus. Ank-H4 and -N5 inhibited processing of compound Relish and reduced the expression of several antimicrobial peptide genes regulated by the Imd signaling pathway in Drosophila mbn2 cells. Studies conducted in the natural host Pseudoplusia includens suggested that parasitism by M. demolitor also activates NF-κB signaling and that MdBV inhibits this response. Overall, our data provide the first quantitative measures of insect and viral IκB binding affinities, while also showing that viral mimics disable Relish processing.

  14. New ISO/TC 163 activities on energy performance of buildings

    NARCIS (Netherlands)

    Dijk, D. van; Spiekman, M.

    2007-01-01

    The International Organization for standardization (ISO), Technical Committee 163 "Thermal Performance and Energy Use in the Built Environment" (ISO/TC 163) has produced and will produce sets of standards by reference to which performance requirements can be expressed at various levels, from

  15. Factors That Relate to the Time to ISO 9000 Registration in Education Institutions

    Science.gov (United States)

    Thonhauser, Theresa

    2008-01-01

    The implementation of the ISO 9000 international quality management system in education has become a debated trend. Building on the literature, this study did not attempt to end the debate over ISO 9000 in education but looked at the problem with a new perspective. The purpose of this study was to identify factors that relate to the time required…

  16. EPA STANDARDS NETWORK FACT SHEET: ISO 14000: INTERNATIONAL ENVIRONMENTAL MANAGEMENT STANDARDS

    Science.gov (United States)

    This flyer provides an overview of the ISO 14000 series of International standards, supplying a brief history, structure of the Technical Committee (TC) 207, structure of the U.S. Technical Advisory Group (TAG) to ISO TC-207, status of the Standards development as of June 1997, w...

  17. A formal analysis of ISO/IEEE P11073-20601 standard of medical device communication

    NARCIS (Netherlands)

    Goga, Nicolae; Costache, Stefania; Moldoveanu, Florica

    2009-01-01

    This article presents the formal work done for the ISO/IEEE P11073-20601 Draft Standard for Health informatics - Personal health device communication - Application profile Optimized exchange protocol. ISO/IEEE 11073 family defines standards for device communication between agents (e.g. blood

  18. ISO 9000 and Malcolm Baldrige: In Training and Education. A Practical Application Guide.

    Science.gov (United States)

    Russo, C. W. Russ

    This book is addressed to educators and work-place trainers interested in applying the Malcolm Baldrige award criteria and/or the International Organization for Standardization (ISO) 9000 training guidelines for program improvement. An initial section compares and contrasts the ISO standards and the Malcolm Baldrige National Quality Award…

  19. Perceptions About The ISO 9000 Quality System Standard Revision And Its Value: The Dutch Experience

    NARCIS (Netherlands)

    A. van der Wiele (Ton); J.D. van Iwaarden (Jos); A.R.T. Williams (Roger); B.G. Dale (Barry)

    2004-01-01

    textabstractThe aim of the research reported in this paper is to assess the relative value of the 2000 version of the ISO 9000 series of quality management system standards in comparison to the 1994 version. 773 organisations in the Netherlands which have all been certified to the ISO 9000 standard

  20. Vergelijking van een tijd besparende analysemethode voor hydroxyproline met ISO 3496-1978

    NARCIS (Netherlands)

    Cazemier, G.; Tuinstra, L.G.M.Th.; Werdmuller, G.A.

    1988-01-01

    De bepaling van hydroxyproline volgens de referentiemethode ISO 3496 is zeer tijdrovend. In een door het Nordisk Metodikkomitti för Livsmedel voorgestelde methode is een aantal wijzigingen ten opzichte van ISO 3496 aangebracht die de analysetijd per monster sterk verkorten. Om na te gaan of deze

  1. The Influence of Academic Culture on Quality Management System ISO 9001 Maintenance within Malaysian Universities

    Science.gov (United States)

    Basir, Siti Arni; Davies, John; Douglas, Jacqueline; Douglas, Alexander

    2017-01-01

    This study investigates the influence of the elements of academic culture on quality management system ISO 9001 maintenance within Malaysian universities. There is a dearth of empirical studies on maintaining ISO 9001, particularly in the higher education context. From the literature review, academic culture was classified according to four…

  2. 78 FR 32384 - New England Power Generators Association v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2013-05-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission New England Power Generators Association v. ISO New England Inc.; Notice of...) filed a formal complaint against ISO New England Inc. (Respondent) alleging that certain newly imposed...

  3. 16 CFR Figure 2 to Part 1203 - ISO Headform-Basic, Reference, and Median Planes

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false ISO Headform-Basic, Reference, and Median Planes 2 Figure 2 to Part 1203 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS SAFETY STANDARD FOR BICYCLE HELMETS Pt. 1203, Fig. 2 Figure 2 to Part 1203—ISO...

  4. The European large area ISO survey - III. 90-mu m extragalactic source counts

    DEFF Research Database (Denmark)

    Efstathiou, A.; Oliver, S.; Rowan-Robinson, M.

    2000-01-01

    We present results and source counts at 90 mum extracted from the preliminary analysis of the European Large Area ISO Survey (ELAIS). The survey covered about 12 deg(2) of the sky in four main areas and was carried out with the ISOPHOT instrument onboard the Infrared Space Observatory (ISO...

  5. ISO 9001 certification for hospitals in Bulgaria: does it help service?

    Science.gov (United States)

    Stoimenova, Assena; Stoilova, Ani; Petrova, Guenka

    2014-03-04

    The aim of our study is to review the published literature on establishment and implementation of ISO 9001 QMS in European hospitals, to study the availability of International Organization for Standardization (ISO) quality management systems (QMS) in Bulgarian hospitals and to outline the main advantages of ISO implementation in the hospitals in Bulgaria. The information on availability of ISO QMS in the hospitals in Bulgaria was gathered via Bulgarian certification register, the registries of various quality associations, websites of hospitals and certification companies presented in Bulgaria. A total number of 312 hospitals in Bulgaria were screened for the availability of QMS certified against the ISO 9001 requirements. The experience of European hospitals that implemented QMS is positive and the used approaches to improve the processes and the demonstrated effects from ISO implementation are analysed by the researchers. Unlike other European Union member states, the establishment of quality management systems in Bulgaria is not compulsory. However, our study revealed that 14.42% of the hospitals in Bulgaria have implemented and have certified quality systems against the requirements of ISO 9001. Our study confirmed that a quality management system using the ISO 9001 standard is useful for the hospitals as it can help to increase the operational efficiencies, to reduce errors, improve patient safety and produce a more preventive approach instead of a reactive environment.

  6. A primer on the Petri Net Markup Language and ISO/IEC 15909-2

    DEFF Research Database (Denmark)

    Hillah, L. M.; Kindler, Ekkart; Kordon, F.

    2009-01-01

    Standard, defines a transfer format for high-level nets. The transfer format defined in Part 2 of ISO/IEC 15909 is (or is based on) the \\emph{Petri Net Markup Language} (PNML), which was originally introduced as an interchange format for different kinds of Petri nets. In ISO/IEC 15909-2, however...

  7. Neurofibromatosis type 2 French cohort analysis using a comprehensive NF2 molecular diagnostic strategy.

    Science.gov (United States)

    Pasmant, E; Louvrier, C; Luscan, A; Cohen, J; Laurendeau, I; Vidaud, M; Vidaud, D; Goutagny, S; Kalamarides, M; Parfait, B

    2015-06-11

    Neurofibromatosis type 2 (NF2) affects about one in 25,000 to 40,000 people. Most NF2 patients have private loss-of-function mutations scattered along the NF2 gene. Here, we present our NF2 investigation strategy. We report a comprehensive NF2 mutation analysis of 221 NF2 French patients: 134 unrelated typical NF2 patients fulfilling the Manchester criteria and 87 unrelated patients presenting symptoms that partially fulfilled the Manchester criteria. A NF2 mutation was identified in 56 of the 221 patients, giving a global mutation detection rate of 25%. This rate reached 37% (49/134) for typical NF2 patients fulfilling the Manchester criteria and only 8% (7/87) for patients presenting symptoms suggestive of NF2. Six of these seven patients were under 25 of age. Our approach showed that 77% of NF2 identified variants were detected by coding exons sequencing. Multiplex ligation-dependent probe amplification allowed the identification of restricted rearrangements (23% of NF2 identified variants corresponding to complete deletion or partial deletion/duplication of NF2). High mutation detection rate can be achieved if well phenotyped NF2 patients are studied with multiple complementary and optimized techniques. NF2 somatic mosaicism detection was improved by frozen tumor samples molecular analysis. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  8. The use of International Standards Organization ISO 9000 quality assurance standards in place of military standards

    Science.gov (United States)

    Beckerdite, Stanley M.

    1992-06-01

    The implementation of quality standards within the European Community by the creation of International Quality Standards 9000 is another step toward development of a global marketplace. It is in the interests of DoD to support this trend in order to help maintain the defense industrial base. The first part of this study performs a comparison of DoD quality standards to the ISO 9000 Standards. The second part of the study consists of a survey of U.S. firms that have become ISO 9000 registered. This survey is intended to provide an assessment of the current movement within the defense industrial base toward adoption of ISO 9000 Standards. The survey also attempts to identify potential implementation issues relating to adoption of ISO 9000 Standards in place of military standards. It is concluded that DoD should implement ISO 9000 and that the impact of this implementation will be favorable.

  9. Evaluation of ISO EN 13606 as a result of its implementation in XML.

    Science.gov (United States)

    Austin, Tony; Sun, Shanghua; Hassan, Taher; Kalra, Dipak

    2013-12-01

    The five parts of the ISO EN 13606 standard define a means by which health-care records can be exchanged between computer systems. Starting within the European standardisation process, it has now become internationally ratified in ISO. However, ISO standards do not require that a reference implementation be provided, and in order for ISO EN 13606 to deliver the expected benefits, it must be provided not as a document, but as an operational system that is not vendor specific. This article describes the evolution of an Extensible Markup Language (XML) Schema through three iterations, each of which emphasised one particular approach to delivering an executable equivalent to the printed standard. Developing these operational versions and incorporating feedback from users of these demonstrated where implementation compromises were needed and exposed defects in the standard. These are discussed herein. They may require a future technical revision to ISO EN 13606 to resolve the issues identified.

  10. PENGARUH CSR BERDASAR ISO 26000 TERHADAP NILAI PERUSAHAAN DENGAN KARAKTERSITIK PERUSAHAAN SEBAGAI VARIABEL MODERATING

    Directory of Open Access Journals (Sweden)

    Sri Wahjuni Latifah

    2015-01-01

    Full Text Available Research of the influence of ISO 26000 CSR toward the company’s value as a moderating variable which is done on companies listed in Indonesia Stock Exchange. The Company’s characteristics are measured by firm age, size, leverage and profitability. The data analysis was done by using double linear regression models, the first is to see the effect based on the ISO 26000 CSR and firm characteristics on value, and the second is to see the effect of interaction with the ISO 26000 corporate characteristics. The results of the study showed that there was no influence of ISO 26000, the characteristics of the company toward the value of the company. However, moderated ISO 26000 by firm characteristics affect the value of the company.

  11. DIN EN ISO 50001. Chances for the forging industry; DIN EN ISO 50001. Chancen fuer die Schmiedeindustrie

    Energy Technology Data Exchange (ETDEWEB)

    Schibisch, Dirk M.; Vathaire, Loic de [SMS Elotherm GmbH, Remscheid (Germany)

    2013-06-15

    The often-quoted 'Energiewende' targets ambitious goals: next to the emission reduction of greenhouse gases by 80 % compared to 1990, also 80 % of Germany's electrical power shall come from renewable energy sources in 2050. The EEG (Renewable Energy law) has been legislated for financing this energy turnaround. Therein the allocation of costs arising from the promotion of power generation from renewables onto the end-consumers is regulated. Production companies with a high energy consumption, which have been certified according to DIN EN ISO 50001, can reduce their power cost, thus maintaining international competitiveness. For the certification incl. the reduction of energy consumption measures for a sustainable boost of energy efficiency are required. This potential, especially interesting for Forge shops, shall be exemplified in this article. (orig.)

  12. La norma ISO 15489-1 en alemán y español: una comparación interlingüística/The Standard ISO 15489-1 in German and Spanish: an Interlinguistic Comparison

    National Research Council Canada - National Science Library

    Patricia Fernández Martín

    2016-01-01

      The aim of this paper is to deepen in the terminology of Records Management established by ISO standards, through a concrete example such as an interlinguistic comparison between UNE ISO 15489-1 and...

  13. Organizational leadership: A study on the affects of certification to International Organization for Standardization (ISO)

    Science.gov (United States)

    Cowart, Jewel S.

    Compliance to requirements of ISO is an important leadership problem for the International Aerospace Quality Group (IAQG) and the Aviation, Space and Defense (AS&D) companies. Furthermore, the IAQG seeks to understand the valuation of ISO/AS9100 requirements (2009), audit practices, management functions, business performance, customer satisfaction, and potential future concepts for the standard to assure quality. Since the release of the AS9100C total certification growth surpassed 1,100,000 organizations in 2010 with ISO 9001 series adoption and implementation governing business operations (ASQ, 2012b; ISO, 2011; Bernardo, Llach, & Marimon, 2011). Historians such as Crosby, Juran, Deming, and others established the foundation for quality assurance. Several researchers explored the issues which complicate overall benefits of ISO certification (Iwaro & Mwasha, 2012; Karthi et al., 2012; Sampaio et al., 2009). This study examined the effects on AS&D industries from ISO compliance to identify the implications (of ever-changing requirements) through an online survey of 15,000 practitioners. The research illuminated how ISO affects AS&D industries with current and future requirements for certification. The data showed that 75% of survey respondents report implementation of the AS9100C requirements still has benefits that outweigh the cost. Findings suggest that AS&D industries perceive significant value in the AS9100C document as part of the overall ISO 9000 series. In general, the comments from the survey can provide insight into the affects of ISO certification. The study concludes by recommending continued research to learn of further impacts from ISO certification within AS&D industries to improve the AS9100 document requirements for quality management systems.

  14. Comparison of ISO standards for device performance; 20072 and 27427: a critical appraisal.

    Science.gov (United States)

    Nerbrink, Ola; Mitchell, Jolyon P

    2012-08-01

    Two separate international standards, ISO 20072:2009 and ISO 27427:2010, have recently been published that relate to the development and performance testing of oral inhaled products (OIPs). The scope of ISO 20072 encompasses all OIP forms except nebulizing systems, whereas ISO 27427 was developed specifically for this class of OIP. Compliance with these standards will likely be necessary for manufacturers seeking approval to market inhaler devices in the European Union (EU). Their adoption in the United States may take a considerable time, but the FDA has expressed support in general terms for the ISO process. Key aspects of both standards that are very different in style and content are identified and discussed from the perspective of a potential user. In the approach adopted by ISO 20072, a formalized risk assessment is undertaken as a key part of design verification, in order to develop the Device Functionality Profile (DFP) of the device. The DFP is subsequently verified by the System Verification Test (SVT), in which pharmacopeial test methods are used to evaluate in vitro performance of the device with a chosen drug product in a statistically robust manner. On the other hand, ISO 27427 adopts a more prescriptive approach that involves performance verification of the finished nebulizing system using 1% w/v salbutamol as the test formulation. Although ISO 27427 is currently undergoing revision, at present it is unclear whether the changes that are made will significantly alter its fundamentally different approach to device performance verification. A strong case can be made for a single OIP-wide ISO standard, based on the principles developed in ISO 20072 and that makes use of the well-understood and validated in vitro test procedures that are available or will shortly be available in the case of nebulizing systems, in the United States and European pharmacopeias.

  15. Blood and urine 8-iso-PGF2α levels in babies of different gestational ages.

    Science.gov (United States)

    Li, Sitao; Hao, Hu; Zhou, Ping; Gao, Ping Ming; Xiao, Xin

    2014-01-01

    We measured cord blood and urine 8-iso-prostaglandin F2α (8-iso-PGF2α) levels in babies of different gestational ages to determine lipid peroxidation status. Babies at gestational ages of 28-43 weeks were divided into group A (28-32 weeks), group B (33-36 weeks), group C (37-41 weeks), and group D (42-43 weeks). 8-iso-PGF2α in umbilical cord blood (UCB) at birth and urine at 6 hours after birth was and tested by ELISA. UCB and urine 8-iso-PGF2α levels in group C were 130.09 ± 31.73 pg/ml and 27.14 ± 6.73 pg/ml, respectively. UCB 8-iso-PGF2α levels in group A and B were 188.42 ± 59.34 pg/ml and 189.37 ± 68.46 pg/ml, and urine 8-iso-PGF2α were 32.14 ± 7.32 pg/ml and 30.46 ± 8.83 pg/ml, respectively. Blood and urine 8-iso-PGF2α levels in group D (post-term) were 252.01 ± 46.42 pg/ml and 44.00 ± 8.50 pg/ml. For all babies, UCB and urine iso-PGF2α levels were significantly correlated (r = 0.65, P iso-PGF2α levels in normal full-term babies. Urine 8-iso-PGF2α levels may reflect the extent of lipid peroxidation in babies. In pre-term and post-term babies, there was evidence for increased lipid peroxidation.

  16. Neuroprotective effects of α-iso-cubebenol on glutamate-induced neurotoxicity.

    Science.gov (United States)

    Park, Sun Young; Choi, Yung Hyun; Park, Geuntae; Choi, Young-Whan

    2015-09-01

    α-Iso-cubebenol is a natural compound isolated from Schisandra chinensis, and is reported to have beneficial bioactivity including anti-inflammatory and anti-tumor activities. Glutamate-induced oxidative neuronal damage has been implicated in a variety of neurodegenerative disorders. Here we investigated the mechanisms of α-iso-cubebenol protection of mouse hippocampus-derived neuronal cells (HT22 cells) from apoptotic cell death induced by the major excitatory neurotransmitter, glutamate. Pretreatment with α-iso-cubebenol markedly attenuated glutamate-induced loss of cell viability and release of lactate dehydrogenase), in a dose-dependent manner. α-Iso-cubebenol significantly reduced glutamate-induced intracellular reactive oxygen species and calcium accumulation. Strikingly, α-iso-cubebenol inhibited glutamate-induced mitochondrial depolarization, which releases apoptosis-inducing factor from mitochondria. α-Iso-cubebenol also suppressed glutamate-induced phosphorylation of extracellular-signal-regulated kinases. Furthermore, α-iso-cubebenol induced CREB phosphorylation and Nrf-2 nuclear accumulation and increased the promoter activity of ARE and CREB in HT22 cells. α-Iso-cubebenol also upregulated the expression of phase-II detoxifying/antioxidant enzymes such as HO-1 and NQO1. Subsequent studies revealed that the inhibitory effects of α-iso-cubebenol on glutamate-induced apoptosis were abolished by small interfering RNA-mediated knockdown of CREB and Nrf-2. These findings suggest that α-iso-cubebenol prevents excitotoxin-induced oxidative damage to neurons by inhibiting apoptotic cell death, and might be a potential preventive or therapeutic agent for neurodegenerative disorders. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Atg7 Regulates Brain Angiogenesis via NF-κB-Dependent IL-6 Production

    Directory of Open Access Journals (Sweden)

    Shi-Fang Zhuang

    2017-05-01

    Full Text Available The formation of brain vasculature is an essential step during central nervous system development. The molecular mechanism underlying brain angiogenesis remains incompletely understood. The role of Atg7, an autophagy-related protein, in brain angiogenesis was investigated in this study. We found that the microvessel density in mice brains with endothelial-specific knockout of Atg7 (Atg7 EKO was significantly decreased compared to wild-type control. Consistently, in vitro angiogenesis assays showed that Atg7 knockdown impaired angiogenesis in brain microvascular endothelial cells. Further results indicated that knockdown of Atg7 reduced interleukin-6 (IL-6 expression in brain microvascular endothelial cells, which is mediated by NF-κB-dependent transcriptional control. Interestingly, exogenous IL-6 restored the impaired angiogenesis and reduced cell motility caused by Atg7 knockdown. These results demonstrated that Atg7 has proangiogenic activity in brain angiogenesis which is mediated by IL-6 production in a NF-κB-dependent manner.

  18. Familial Lymphoproliferative Malignancies and Tandem Duplication of NF1 Gene

    Directory of Open Access Journals (Sweden)

    Gustavo Fernandes

    2014-01-01

    Full Text Available Background. Neurofibromatosis type 1 is a genetic disorder caused by loss-of-function mutations in a tumor suppressor gene (NF1 which codifies the protein neurofibromin. The frequent genetic alterations that modify neurofibromin function are deletions and insertions. Duplications are rare and phenotype in patients bearing duplication of NF1 gene is thought to be restricted to developmental abnormalities, with no reference to cancer susceptibility in these patients. We evaluated a patient who presented with few clinical signs of neurofibromatosis type 1 and a conspicuous personal and familiar history of different types of cancer, especially lymphoproliferative malignancies. The coding region of the NF-1 gene was analyzed by real-time polymerase chain reaction and direct sequencing. Multiplex ligation-dependent probe amplification was performed to detect the number of mutant copies. The NF1 gene analysis showed the following alterations: mosaic duplication of NF1, TRAF4, and MYO1D. Fluorescence in situ hybridization using probes (RP5-1002G3 and RP5-92689 flanking NF1 gene in 17q11.2 and CEP17 for 17q11.11.1 was performed. There were three signals (RP5-1002G3conRP5-92689 in the interphases analyzed and two signals (RP5-1002G3conRP5-92689 in 93% of cells. These findings show a tandem duplication of 17q11.2. Conclusion. The case suggests the possibility that NF1 gene duplication may be associated with a phenotype characterized by lymphoproliferative disorders.

  19. Two alleles of NF-kappaB in the sea anemone Nematostella vectensis are widely dispersed in nature and encode proteins with distinct activities.

    Directory of Open Access Journals (Sweden)

    James C Sullivan

    2009-10-01

    Full Text Available NF-kappaB is an evolutionarily conserved transcription factor that controls the expression of genes involved in many key organismal processes, including innate immunity, development, and stress responses. NF-kappaB proteins contain a highly conserved DNA-binding/dimerization domain called the Rel homology domain.We characterized two NF-kappaB alleles in the sea anemone Nematostella vectensis that differ at nineteen single-nucleotide polymorphisms (SNPs. Ten of these SNPs result in amino acid substitutions, including six within the Rel homology domain. Both alleles are found in natural populations of Nematostella. The relative abundance of the two NF-kappaB alleles differs between populations, and departures from Hardy-Weinberg equilibrium within populations indicate that the locus may be under selection. The proteins encoded by the two Nv-NF-kappaB alleles have different molecular properties, in part due to a Cys/Ser polymorphism at residue 67, which resides within the DNA recognition loop. In nearly all previously characterized NF-kappaB proteins, the analogous residue is fixed for Cys, and conversion of human RHD proteins from Cys to Ser at this site has been shown to increase DNA-binding ability and increase resistance to inhibition by thiol-reactive compounds. However, the naturally-occurring Nematostella variant with Cys at position 67 binds DNA with a higher affinity than the Ser variant. On the other hand, the Ser variant activates transcription in reporter gene assays more effectively, and it is more resistant to inhibition by a thiol-reactive compound. Reciprocal CysSer mutations at residue 67 of the native Nv-NF-kappaB proteins affect DNA binding as in human NF-kappaB proteins, e.g., a Cys->Ser mutation increases DNA binding of the native Cys variant.These results are the first demonstration of a naturally occurring and functionally significant polymorphism in NF-kappaB in any species. The functional differences between these alleles

  20. DIRKS: putting ISO 15489 to work: any records manager using ISO 15489 to develop a new program or evaluate an existing one should become familiar with the DIRKS implementation process

    National Research Council Canada - National Science Library

    Macintosh, Stephen; Real, Lynne

    2007-01-01

    ... implementation document for ISO 15489: ISO/TR 15489-2 Information and Documentation--Records Management--Part 2: Guidelines. Any records manager using ISO 15489--either in developing a new program or evaluating an existing one--should become familiar with the related implementation process known as DIRKS (Developing and Implementing a Recordkeeping Sy...

  1. Lateral flow assays.

    Science.gov (United States)

    Koczula, Katarzyna M; Gallotta, Andrea

    2016-06-30

    Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored. © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

  2. Lateral flow assays

    Science.gov (United States)

    Koczula, Katarzyna M.

    2016-01-01

    Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored. PMID:27365041

  3. Tube-Forming Assays.

    Science.gov (United States)

    Brown, Ryan M; Meah, Christopher J; Heath, Victoria L; Styles, Iain B; Bicknell, Roy

    2016-01-01

    Angiogenesis involves the generation of new blood vessels from the existing vasculature and is dependent on many growth factors and signaling events. In vivo angiogenesis is dynamic and complex, meaning assays are commonly utilized to explore specific targets for research into this area. Tube-forming assays offer an excellent overview of the molecular processes in angiogenesis. The Matrigel tube forming assay is a simple-to-implement but powerful tool for identifying biomolecules involved in angiogenesis. A detailed experimental protocol on the implementation of the assay is described in conjunction with an in-depth review of methods that can be applied to the analysis of the tube formation. In addition, an ImageJ plug-in is presented which allows automatic quantification of tube images reducing analysis times while removing user bias and subjectivity.

  4. SINERGI ISO 9001:2000 - CMMI PADA INDUSTRI PENGEMBANG PERANGKAT LUNAK

    Directory of Open Access Journals (Sweden)

    Leo Willyanto Santoso

    2005-01-01

    Full Text Available All sectors of society including softwere industry, have a big concern about quality, both of product quality and service quality. The need to manage risk, has a strong relation with quality and increases system complexity. Standarization in determining quality, ISO is a must for all software developer. If we study further, ISO 9001:2000 still has some weakness especially for implementation in software product. This paper presents a comparative analysis of quality approaches to risk management. This paper also provide a general overview of all the risks aspects treated in ISO 9001:2000 standards and CMMI model version 1.1. In this research, we obtain a strategy to implement ISO 9001:2000 and CMMI together when we make a planing to increase software process. Besides it produces analysis how to change position from ISO 9001:2000 to CMMI. In the implementation on software industry PT 'X', obtain get various benefits that has never been obtained before. Abstract in Bahasa Indonesia : Semua hal dalam hidup ini selalu memperhatikan masalah kualitas, baik itu berupa kualitas produk ataupun kualitas layanan. Pada industri perangkat lunak, hal ini tidak dibedakan, kebutuhan untuk menghasilkan produk yang berkualitas akan meningkatkan kekompleksan dari sebuah sistem. Standarisasi dalam penentuan kualitas, ISO sudah menjadi keharusan bagi para pengembang perangkat lunak. Apabila diteliti lebih lanjut, ISO 9001:2000 masih mempunyai banyak kekurangan, khususnya untuk diaplikasikan dalam produk perangkat lunak.Pada penelitian ini, akan dibahas analisis perbandingan dengan melakukan pendekatan kualitas untuk meningkatkan proses perangkat lunak dengan melakukan mapping antara ISO 9001:2000 dengan CMMI. Hal-hal yang dibahas berupa aspek-aspek dari standard ISO dan CMMI versi 1.1. Pada penelitian ini, dapat diperoleh strategi untuk menerapkan ISO 9001:2000 dan CMMI secara sinergi ketika merencanakan untuk melakukan peningkatan proses perangkat lunak. Selain itu

  5. Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

    Directory of Open Access Journals (Sweden)

    Heema Hewitson

    2016-03-01

    Full Text Available The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739–3742 ΔTTTG. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

  6. Modelling the comet assay.

    Science.gov (United States)

    McArt, Darragh G; McKerr, George; Howard, C Vyvyan; Saetzler, Kurt; Wasson, Gillian R

    2009-08-01

    The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing all the cellular content except the DNA. By relaxing the DNA in an alkaline buffer, strands containing breaks are released from supercoiling. Upon electrophoresis, these strands are pulled out into the agarose, forming a tail which, when stained with a fluorescent dye, can be analysed by fluorescence microscopy. The intensity of this tail reflects the amount of DNA damage sustained. Despite being such an established and widely used assay, there are still many aspects of the comet assay which are not fully understood. The present review looks at how the comet assay is being used, and highlights some of its limitations. The protocol itself varies among laboratories, so results from similar studies may vary. Given such discrepancies, it would be attractive to break the assay into components to generate a mathematical model to investigate specific parameters.

  7. Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Gong-Min Chang

    2015-01-01

    Full Text Available The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002. Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4Gy group, and neutron irradiation of 4Gy with LY294002 treatment group. Except the control group, the other groups were irradiated by neutron of 4Gy. LY294002 was given before 24 hours of neutron irradiation. At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected. At 6 h and 24 h after neutron irradiation of 4Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found. The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups. The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.

  8. The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF-κB.

    Science.gov (United States)

    Fathy, S A; Emam, M A; Abo Agwa, S H; Abu Zahra, F A; Youssef, F S; Sami, R M

    2016-08-31

    Hepatocellular carcinoma (HCC) is one of the most common cancer types with a high prevalence and itis the leading cause of cancer deaths worldwide. This study aimed to investigate the antiproliferative effect of aqueous and ethanol extracts of Origanum majorana leaf on human hepatocellular carcinoma (HepG2) cell line through incubation of various concentrations of origanum majorana extracts with HepG2 and at different time intervals. The effects of aqueous and ethanol extracts of O. majorana L. on HepG2 cell viability, nuclear factor kappa B (NF-kB) gene expression were examined. The results of the cell viability assays showed that aqueous and ethanol extracts exhibited a highly significant inhibitory effect on HepG2 cell proliferation which was evidenced by a reduction in viable cell count. The results were confirmed by microscopical examination of cell morphology. Furthermore, the O. majorana L. extracts suppressed the activity of NF-kB gene expression of HepG2 cells compared to the control.The conclusions from this study suggest that marjoram extracts exhibit anti-proliferative effect against HCC through suppressing the activity of NF-kB gene expression and high antioxidant activity.

  9. Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif.

    Science.gov (United States)

    Toki, T; Itoh, J; Kitazawa, J; Arai, K; Hatakeyama, K; Akasaka, J; Igarashi, K; Nomura, N; Yokoyama, M; Yamamoto, M; Ito, E

    1997-04-24

    The transcription factor NF-E2, a heterodimeric protein complex composed of p45 and small Maf family proteins, is considered crucial for the regulation of erythroid gene expression and platelet formation. To facilitate the characterization of NF-E2 functions in human cells, we isolated cDNAs encoding two members of the small Maf family, MafK and MafG. The human mafK and mafG genes encode proteins of 156 and 162 amino acid residues, respectively, whose deduced amino acid sequences show approximately 95% identity to their respective chicken counterparts. Expression of mafK mRNA is high in heart, skeletal muscle and placenta, whereas mafG mRNA is abundant in skeletal muscle and is moderately expressed in heart and brain. Both are expressed in all hematopoietic cell lines, including those of erythroid and megakaryocytic lineages. In electrophoretic gel mobility shift assays binding to NF-E2 sites was found to depend on formation of homodimers or heterodimers with p45 and p45-related CNC family proteins. The results suggest that the small Maf family proteins function in human cells through interaction with various basic-leucine zipper-type transcription factors.

  10. Identification and characterization of NF-Y transcription factor families in Canola (Brassica napus L.).

    Science.gov (United States)

    Liang, Mingxiang; Yin, Xiangzhen; Lin, Zhongyuan; Zheng, Qingsong; Liu, Guohong; Zhao, Gengmao

    2014-01-01

    NF-Y (NUCLEAR FACTOR-Y), a heterotrimeric transcription factor, is composed of NF-YA, NF-YB, and NF-YC proteins in yeast, animal, and plant systems. In plants, each of the NF-YA/B/C subunit forms a multi-member family. NF-Ys are key regulators with important roles in many physiological processes, such as drought tolerance, flowering time, and seed development. In this study, we identified, annotated, and further characterized 14 NF-YA, 14 NF-YB, and 5 NF-YC proteins in Brassica napus (canola). Phylogenetic analysis revealed that the NF-YA/B/C subunits were more closely clustered with the Arabidopsis thaliana (Arabidopsis) homologs than with rice OsHAP2/3/5 subunits. Analyses of the conserved domain indicated that the BnNF-YA/B/C subfamilies, respectively, shared the same conserved domains with those in other organisms, including Homo sapiens, Saccharomyces cerevisiae, Arabidopsis, and Oryza sativa (rice). An examination of exon/intron structures revealed that most gene structures of BnNF-Y were similar to their homologs in Arabidopsis, a model dicot plant, but different from those in the model monocot plant rice, suggesting that plant NF-Ys diverged before monocot and dicot plants differentiated. Spatial-tempo expression patterns, as determined by qRT-PCR, showed that most BnNF-Ys were widely expressed in different tissues throughout the canola life cycle and that several closely related BnNF-Y subunits had similar expression profiles. Based on these findings, we predict that BnNF-Y proteins have functions that are conserved in the homologous proteins in other plants. This study provides the first extensive evaluation of the BnNF-Y family, and provides a useful foundation for dissecting the functions of BnNF-Y.

  11. Association study of the NF1 gene and autistic disorder.

    Science.gov (United States)

    Mbarek, O; Marouillat, S; Martineau, J; Barthélémy, C; Müh, J P; Andres, C

    1999-12-15

    Neurofibromatosis type 1 (NF1) is increased about 150-fold in autistic patients. The aim of this study was to test for an association between the NF1 locus and autistic disorder. The allele distributions of three markers of the NF1 gene were studied in 85 autistic patients and 90 controls. No differences in allele distributions were observed. However, we found a new allele (allele 5) of the GXAlu marker in four autistic patients. Allele 5 was absent in a larger control population (213 individuals). The patients with allele 5 had a more severe clinical picture, mainly in the fields of motility and tonus. Our preliminary results suggest that the NF1 region is not a major susceptibility locus for autism. However, the GXAlu marker of the NF1 gene appears as a possible candidate for a susceptibility locus in a small subgroup of severely affected autistic patients. Am. J. Med. Genet. (Neuropsychiatr. Genet.) 88:729-732, 1999. Copyright 1999 Wiley-Liss, Inc.

  12. WIAMan Technology Demonstrator Sensor Codes Conforming to International Organization for Standardization/Technical Standard (ISO/TS) 13499

    Science.gov (United States)

    2016-03-01

    Standardization/Technical Standard (ISO/ TS ) 13499 by Michael Tegtmeyer Approved for public release; distribution is unlimited. NOTICES Disclaimers The findings...International Organization for Standardization/Technical Standard (ISO/ TS ) 13499 by Michael TegtmeyerWIAMan Engineering Office, ARL Approved for public...WIAMan Technology Demonstrator Sensor Codes Conforming to International Organization for Standardization/Technical Standard (ISO/ TS ) 13499 Michael

  13. Mapping ‘hydroscapes’ along the iso- to anisohydric continuum of stomatal regulation of plant water status

    Science.gov (United States)

    Frederick C. Meinzer; David R. Woodruff; Danielle E. Marias; Duncan D. Smith; Katherine A. McCulloh; Ava R. Howard; Alicia L. Magedman; Josep Penuelas

    2016-01-01

    The concept of iso- vs. anisohydry has been used to describe the stringency of stomatal regulation of plant water potential (ψ). However, metrics that accurately and consistently quantify species’ operating ranges along a continuum of iso- to anisohydry have been elusive. Additionally, most approaches to quantifying iso/anisohydry require labour-intensive measurements...

  14. 76 FR 7839 - Notice of Commission and Commission Staff Attendance at ISO/RTO Council and Regional State...

    Science.gov (United States)

    2011-02-11

    ... Energy Regulatory Commission Notice of Commission and Commission Staff Attendance at ISO/RTO Council and... of the Commission and Commission staff may attend the following ISO/RTO Council and Regional State..., RTO/ISO Performance Metrics. For more information, contact Sandra Waldstein, Office of External...

  15. 75 FR 3225 - Notice of Commission Staff Attendance at Organization of MISO States and Midwest ISO Meetings

    Science.gov (United States)

    2010-01-20

    ... Midwest ISO Meetings January 12, 2010. The Federal Energy Regulatory Commission hereby gives notice that....m. CT All meetings scheduled to be held in Carmel will take place at: Midwest ISO Headquarters, 720.... LLC v. Midwest Independent Transmission System Operator, Inc. Docket No. ER08-15, Midwest ISO...

  16. A debranching enzyme IsoM of Corallococcus sp. strain EGB with potential in starch processing.

    Science.gov (United States)

    Li, Zhoukun; Ji, Kai; Zhou, Jie; Ye, Xianfeng; Wang, Ting; Luo, Xue; Huang, Yan; Cao, Hui; Cui, Zhongli; Kong, Yi

    2017-12-01

    Interest in use of resistant starch and maltooligosaccharides as functional foods and biopreservatives has grown in recent years. In this research, a novel debranching enzyme IsoM from Corallococcus sp. strain EGB was identified and expressed in P. pastoris GS115. Sequence alignments showed that IsoM was typical isoamylase with the specific activity up to 70,600U/mg, which belongs to glycoside hydrolase family 13 (GH 13). Enzymatic reaction pattern demonstrated that IsoM has high debranching efficiency against α-1,6-glycosidic bond of branched starch, and exhibited no activity towards α-1,4-glycosidic bond. The potential application of IsoM in starch processing was determined. IsoM was a potential candidate for the production of RS (70.9%) from raw starch, which was comparable with the commercial pullulanase (Promozyme(®)D2). IsoM also improved the maltohexaose yield in combination with maltohexaose-producing α-amylase AmyM (KM114206), the maltohexaose yield was improved by 63.3% compared with 21.9% improvement of Promozyme(®)D2. The results of RS production and combination with other amylases suggesting that IsoM is a potential candidate for the efficient conversion of starch. Copyright © 2017. Published by Elsevier B.V.

  17. Development of an ISO 9000 compatible occupational health standard: defining the issues.

    Science.gov (United States)

    Dyjack, D T; Levine, S P

    1995-06-01

    Corporate ISO 9000 registration is gaining international acceptance as the hallmark of quality system achievement. The International Organization for Standardization (ISO) is currently drafting environmental standards that will complement ISO 9000. Should the international community also consider development of an ISO 9000-compatible occupational safety and health management standard (OSHMS)? To determine the advantages and disadvantages of this issue, the investigators conducted interviews with government and private sector experts, reviewed publicly accessible ISO documents, and evaluated published literature germane to the subject. Major advantages of an ISO OSHMS were the harmonization of national standards, maximizing Occupational Safety and Health Administration (OSHA) efficiency through third-party registration audits, and increased emphasis on employee-driven health and safety programs. Major disadvantages were the single vote of the American National Standards Institute at international proceedings, direct and indirect program development costs, potential unethical or incompetent conduct of registrars, and the logistics of developing an acceptable standard to all stakeholders. Some unresolved issues were the inevitability of an ISO OSHMS, auditor indemnification, and the scope of OSHA participation. Industrial health and safety professionals should initiate formal discussion on this issue to elaborate on findings presented here and to establish a consensus on future activities.

  18. Sustainable development induction in organizations: a convergence analysis of ISO standards management tools' parameters.

    Science.gov (United States)

    Merlin, Fabrício Kurman; Pereira, Vera Lúciaduarte do Valle; Pacheco, Waldemar

    2012-01-01

    Organizations are part of an environment in which they are pressured to meet society's demands and acting in a sustainable way. In an attempt to meet such demands, organizations make use of various management tools, among which, ISO standards are used. Although there are evidences of contributions provided by these standards, it is questionable whether its parameters converge for a possible induction for sustainable development in organizations. This work presents a theoretical study, designed on structuralism world view, descriptive and deductive method, which aims to analyze the convergence of management tools' parameters in ISO standards. In order to support the analysis, a generic framework for possible convergence was developed, based on systems approach, linking five ISO standards (ISO 9001, ISO 14001, OHSAS 18001, ISO 31000 and ISO 26000) with sustainable development and positioning them according to organization levels (strategic, tactical and operational). The structure was designed based on Brundtland report concept. The analysis was performed exploring the generic framework for possible convergence based on Nadler and Tushman model. The results found the standards can contribute to a possible sustainable development induction in organizations, as long as they meet certain minimum conditions related to its strategic alignment.

  19. Specification and tolerancing of bulk glass material imperfections with ISO standards

    Science.gov (United States)

    Krisiloff, Allen J.

    2017-08-01

    Standards for the specification of tolerances for glass material imperfections have been evolving over the past 40 years. Today, several individual ISO Standards for drawings and drawing notation - ISO 10110-2, ISO-10110-3, and ISO- 10110-4, which were last revised in 1996 and 1997 - are being merged and re-written to incorporate technical improvements and enhance the clarity of presentation. The new standard, tentatively numbered ISO 10110-18, is on schedule for release in 2018. It will also provide notation to directly utilize concepts and quality classes defined in ISO 12123, the newly revised standard for raw glass material. New ways to specify striae and a way to specify raw material specifications on a finished part drawing are two additional highlights of the revised versions of this set of ISO standards. This paper will discuss the old shortcomings, their corrections, and the new features incorporated into the set of standards currently under final development and whose publication is expected next year.

  20. Design, synthesis, and biological evaluation of benzofuran- and 2,3-dihydrobenzofuran-2-carboxylic acid N-(substituted)phenylamide derivatives as anticancer agents and inhibitors of NF-κB.

    Science.gov (United States)

    Choi, Minho; Jo, Hyeju; Park, Hyun-Jung; Sateesh Kumar, Arepalli; Lee, Joonkwang; Yun, Jieun; Kim, Youngsoo; Han, Sang-Bae; Jung, Jae-Kyung; Cho, Jungsook; Lee, Kiho; Kwak, Jae-Hwan; Lee, Heesoon

    2015-06-15

    With the aim of developing novel scaffolds as anticancer agents and inhibitors of NF-κB activity, 60 novel benzofuran- and 2,3-dihydrobenzofuran-2-carboxylic acid N-(substituted)phenylamide derivatives (1a-s, 2a-k, 3a-s, and 4a-k) were designed and synthesized from the reference lead compound KL-1156, which is an inhibitor of NF-κB translocation to the nucleus in LPS-stimulated RAW 264.7 macrophage cells. The novel benzofuran- and 2,3-dihydrobenzofuran-2-carboxamide derivatives exhibited potent cytotoxic activities (measured by the sulforhodamine B assay) at low micromolar concentrations against six human cancer cell lines: ACHN (renal), HCT15 (colon), MM231 (breast), NUGC-3 (gastric), NCI-H23 (lung), and PC-3 (prostate). In addition, these compounds also inhibited LPS-induced NF-κB transcriptional activity. The +M effect and hydrophobic groups on the N-phenyl ring potentiated the anticancer activity and NF-κB inhibitory activity, respectively. However, according to the results of structure-activity relationship studies, only benzofuran-2-carboxylic acid N-(4'-hydroxy)phenylamide (3m) was the lead scaffold with both an outstanding anticancer activity and NF-κB inhibitory activity. This novel lead scaffold may be helpful for investigation of new anticancer agents that act through inactivation of NF-κB. Copyright © 2015 Elsevier Ltd. All rights reserved.