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Sample records for aspe valley pyrenees

  1. Characterisation and quantification of trace metal elements in atmospheric deposition and particularities in the Aspe valley (Pyrenees): implementation of road traffic air quality indicators

    International Nuclear Information System (INIS)

    Veschambre, S.

    2006-04-01

    This study of inputs of trace metal elements (TME) in the Aspe valley (Pyrenees Atlantiques) has two objectives: (1) to define a reference state of metallic contaminants for the monitoring of road traffic emissions since the opening of the Somport tunnel and, (2) to evaluate sources and climatic conditions which contribute to TME inputs in the Aspe valley. To establish air quality indicators, TME (Al, Na, Mg, K, V, Mn, Cr, Zn, Cu, Rb, Cd, Sn, Sb, Ba, Ce, Pb and U) and lead isotopic ratios ( 208 Pb/ 206 Pb, 206 Pb/ 207 Pb and 208 Pb/ 207 Pb) were determined in the atmospheric receptors (fresh snow, wet deposition, atmospheric particulates and lichen). Sampling and analyses with ultra clean procedures were employed for TME quantification. Variability of atmospheric receptors studied, allows integration on a daily and pluri-annual temporal scale and a spatial scale in the North-South axis of the valley and as a function of the altitude from the road. The Aspe valley presents a level of contamination characteristic of remote European areas and the metallic contaminants identified are Cd, Sb, Zn, Cu, Pb and Sn. In the low valley, air quality indicators indicate contaminant contributions (i) from local emissions of domestic heat sources, from agricultural burning practices and road traffic, and (ii) from regional anthropogenic sources of waste incinerators, metallurgic industries and urban centres. In altitude, the valley is significantly influenced by wind erosion and long range transport of TME in the Northern Hemisphere. Characterisation of TME and the isotopic ratios of Pb in the Somport tunnel indicate (i) a significant emission of Cu, Sb, Zn and Ba and (ii) an isotopic composition from a slightly radiogenic source even though Pb concentrations indicate low emissions from road traffic emissions. Nevertheless, the low traffic volume in the Aspe valley prevents conclusive evidence of significant contamination from road traffic. (author)

  2. Characterisation and quantification of trace metal elements in atmospheric deposition and particularities in the Aspe valley (Pyrenees): implementation of road traffic air quality indicators; Caracterisation et quantification des elements traces metalliques dans les depots et les particules atmospheriques de la vallee d'Aspe (Pyrenees): Mise en place d'indicateurs de la qualite de l'air lies au trafic routier

    Energy Technology Data Exchange (ETDEWEB)

    Veschambre, S

    2006-04-15

    This study of inputs of trace metal elements (TME) in the Aspe valley (Pyrenees Atlantiques) has two objectives: (1) to define a reference state of metallic contaminants for the monitoring of road traffic emissions since the opening of the Somport tunnel and, (2) to evaluate sources and climatic conditions which contribute to TME inputs in the Aspe valley. To establish air quality indicators, TME (Al, Na, Mg, K, V, Mn, Cr, Zn, Cu, Rb, Cd, Sn, Sb, Ba, Ce, Pb and U) and lead isotopic ratios ({sup 208}Pb/{sup 206}Pb, {sup 206}Pb/{sup 207}Pb and {sup 208}Pb/{sup 207}Pb) were determined in the atmospheric receptors (fresh snow, wet deposition, atmospheric particulates and lichen). Sampling and analyses with ultra clean procedures were employed for TME quantification. Variability of atmospheric receptors studied, allows integration on a daily and pluri-annual temporal scale and a spatial scale in the North-South axis of the valley and as a function of the altitude from the road. The Aspe valley presents a level of contamination characteristic of remote European areas and the metallic contaminants identified are Cd, Sb, Zn, Cu, Pb and Sn. In the low valley, air quality indicators indicate contaminant contributions (i) from local emissions of domestic heat sources, from agricultural burning practices and road traffic, and (ii) from regional anthropogenic sources of waste incinerators, metallurgic industries and urban centres. In altitude, the valley is significantly influenced by wind erosion and long range transport of TME in the Northern Hemisphere. Characterisation of TME and the isotopic ratios of Pb in the Somport tunnel indicate (i) a significant emission of Cu, Sb, Zn and Ba and (ii) an isotopic composition from a slightly radiogenic source even though Pb concentrations indicate low emissions from road traffic emissions. Nevertheless, the low traffic volume in the Aspe valley prevents conclusive evidence of significant contamination from road traffic. (author)

  3. Studies on pharmaceutical ethnobotany in the high river Ter valley (Pyrenees, Catalonia, Iberian Peninsula).

    Science.gov (United States)

    Rigat, Montse; Bonet, Maria Angels; Garcia, Sònia; Garnatje, Teresa; Vallès, Joan

    2007-09-05

    An ethnobotanical study has been carried out in the high river Ter valley (Catalonia, Iberian Peninsula) a small area located in the eastern Pyrenees, with 294 km(2) and 4526 inhabitants. Through 42 interviews with 60 informants of a mean age of 71.1, 220 species belonging to 71 botanical families were reported, 90.6% of which were used in human medicine and 7.8% in veterinary therapy. The present paper is focused on human medicinal plant uses. One fungal and four vascular plant species have not, or have very rarely been cited as medicinal, and for other taxa some very scarcely reported medicinal uses have been recorded (110 uses concerning 78 species).

  4. Glacial stages and post-glacial environmental evolution in the Upper Garonne valley, Central Pyrenees.

    Science.gov (United States)

    Fernandes, M; Oliva, M; Palma, P; Ruiz-Fernández, J; Lopes, L

    2017-04-15

    The maximum glacial extent in the Central Pyrenees during the Last Glaciation is known to have occurred before the global Last Glacial Maximum, but the succession of cold events afterwards and their impact on the landscape are still relatively unknown. This study focuses on the environmental evolution in the upper valley of the Garonne River since the Last Glaciation. Geomorphological mapping allows analysis of the spatial distribution of inherited and current processes and landforms in the study area. The distribution of glacial records (moraines, till, erratic boulders, glacial thresholds) suggests the existence of four glacial stages, from the maximum expansion to the end of the glaciation. GIS modeling allows quantification of the Equilibrium Line Altitude, extent, thickness and volume of ice in each glacial stage. During the first stage, the Garonne glacier reached 460m in the Loures-Barousse-Barbazan basin, where it formed a piedmont glacier 88km from the head and extended over 960km 2 . At a second stage of glacier stabilization during the deglaciation process, the valley glaciers were 12-23km from the head until elevations of 1000-1850m, covering an area of 157km 2 . Glaciers during stage three remained isolated in the upper parts of the valley, at heights of 2050-2200m and 2.6-4.5km from the head, with a glacial surface of 16km 2 . In stage four, cirque glaciers were formed between 2260m and 2590m, with a length of 0.4-2km and a glacial area of 5.7km 2 . Also, the wide range of periglacial, slope, nival and alluvial landforms existing in the formerly glaciated environments allows reconstruction of the post-glacial environmental dynamics in the upper Garonne basin. Today, the highest lands are organized following three elevation belts: subnival (1500-1900m), nival (1900-2300m) and periglacial/cryonival (2300-2800m). Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Soil properties relevant to land degradation in abandoned sloping fields in Aisa valley, Central Pyrenees (Spain

    Directory of Open Access Journals (Sweden)

    Pardini, G.

    1991-06-01

    Full Text Available A multi-approach characterization of soil properties in abandoned fields in the Aisa valley, at mid mountain in the Central Spanish Pyrenees, demonstrated that the soil's own peculiar characteristics are concerned with conservation problems. Aggregate stability and shrinkage tests pointed to a relatively good soil performance due to the aggregating role of organic matter and calcium carbonates, although calcium ions, in some instances, may exert and additional antagonistic role for a sealed surface, increasing runoff. On the other hand, soil micromorphology suggests that the poor condition of the soils is in some contradiction to paedogenic activity. These findings, together with the presence of ashes, support the hypothesis that land degradation in these areas is mainly related to human activity thought unsuitable management after land abandonment.

    [es] La caracterización de diversas propiedades del suelo en campos abandonados del valle de Aisa, montaña media del Pirineo Central, ha mostrado que dichos suelos presentan algunos caracteres de interés desde el punto de vista de la conservación. La estabilidad de los agregados y los test de agrietamiento evidencian un comportamiento aceptable, gracias al papel agregante de la materia orgánica y carbonatos de calcio, a pesar que los iones calcio, en algunas ocasiones, pueden ejercer un papel antagonista adicional y favorecer el sellado de la superficie del suelo, aumentando la escorrentía superficial. Por otra parte, la micromorfología sugiere que el estado de degradación de los suelos contrasta con la actividad pedogénica. Estos resultados, juntamente con la presencia de cenizas, apoyan la hipótesis de que el estado de degradación en estas áreas es consecuencia principalmente de una utilización incorrecta después del abandono de los cultivos.
    [fr] Un étude des propriétés des sois dans une zone à cultures en pente abandonnées dans la vallée d'Aisa (Pyr

  6. Co-evolution of soils and vegetation in the Aísa Valley Experimental Station (Central Pyrenees)

    Science.gov (United States)

    Serrano Muela, Maria Pilar; Nadal Romero, Estela; Lasanta, Teodoro; María García Ruiz, José

    2013-04-01

    Soils and vegetation tend to evolve jointly in relation to climate evolution and the impacts of human activity. This study analyzes soil and vegetation characteristics under various plant covers, using information from the Aísa Valley Experimental Station (AVES), Spanish Pyrenees, from 1991 to 2010. The land uses considered were: dense shrub cover, grazing meadow, abandoned field, cereal (barley), abandoned shifting agriculture, active shifting agriculture, burnt1 and burnt2 plots, and in-fallow plot. All the plots were installed on a field abandoned 45 years ago. Some of the plots did not change in plant cover through the study period (e.g., the meadow, cereal and shifting agriculture plots), but others underwent changes in density and composition, such as: (i) The dense shrub cover plot represents the natural evolution of the abandoned field. When the AVES was equipped, this plot was completely dominated by Genista scorpius, with a few stands of Rosa gr. Canina. Twenty years later, Genista scorpius is affected of senescence and shows almost no regeneration capacity. (ii) The abandoned field had previously been cultivated with cereals until 1993. Once abandoned, the progression of plant colonization was very rapid. Firstly with grasses and, 10 years later, with Genista scorpius. At present, this latter occupies more than 50% of the plot. (iii) The evolution of plant colonization in the abandoned shifting agriculture plot was slower than that in the 'normal' abandoned field, mainly because of the differences in fertilization when they were cultivated. (iv) One of the burnt plots evolved from 0% to a coverage of almost 100% in a shot period, whereas the other plot remained with a shrub density of about 60% several years after the fire. Soil samples (superficial and depth) were analyzed to obtain physical and chemical properties: structure, texture, pH, CaCO3, Organic Matter and various anions and cations. The main purpose was to detect differences in the soil

  7. Glacial evolution of the upper Gallego Valley (Panticosa mountains and Ribera de Biescas, Aragonese Pyrenees, Spain

    Directory of Open Access Journals (Sweden)

    Serrano-Cañadas, Enrique

    1991-12-01

    Full Text Available Glacial evolution in the upper Gallego Valley has been established by studying erosional and depositional land forms. Ten pulsations, related to five phases are described: Premaximal (F. G. 0, attributed to Middle Pleistocene; Peniglacier, with three expanding pulsations (F.G. 1, 2 y 3, is attributed to the Upper Pleistocene; Finipleniglacial with two phases of dynamic (F.G. 4 y 5' and climatic (F.G. 5 equilibrium associated with the Pleistocene deglaciation; high mountain phase (F.G. 6 y 7, with two morphogenetic episodes; and the Holocene pulses from the Little Ice Age.

    [es] Evolución glaciar del Alto Gallego (Montañas de Panticosa y Ribera de Biescas, Pirineo aragonés. Se reconstruye la evolución glaciar del Alto Gallego a partir del estudio de las formas de erosión y acumulación glaciar. Se describen diez pulsaciones, correspondientes a cinco fases mayores: Premáximo (F.G.O., atribuido al Pleistoceno medio; Pleniglaciar, con tres pulsaciones de expansión (F.G. 1,2 y 3, del Pleistoceno reciente; el Finipleniglaciar,con fases de equilibrio dinámico (F.G. 4y 5'y climático (F.G.S, ligadas a la deglaciación pleistocena; las fases de alta montaña, con dos pulsaciones (F.G. 6 y 7, atribuidas al Tardiglaciar; y las fases holocenas, de la Pequeña Edad del Hielo.
    [fr] Évolution glaciaire de Haut Gallego (montagnes de Panticosa et Rivage de Biescas, Pyrénées aragonaises. On étudie l'évolution glaciaire du Haut Gallego à partir de l'étude des formes d'érosion et accumulation glaciaire. On décrit dix pulsations, correspondant à cinq phases majeures: le Prémaximun (F.G. 0, attribué au Pléistocène moyen; le Pléniglaciaire avec trois pulsations d'expansion (F.G. 1, 2 et 3 attribuée au Pleistocene récent; le Pini-pléniglaciaire avec des phases d'équilibre dynamique (F.G. 4 et 5 et climatique (F.G. 5, liées à la déglaciation pléistocène; les phases de haute montagne, avec deux pulsations (F.G. 6 et 7 attribu

  8. Glaciolacustrine deposits formed in an ice-dammed tributary valley in the south-central Pyrenees: New evidence for late Pleistocene climate

    Science.gov (United States)

    Sancho, Carlos; Arenas, Concha; Pardo, Gonzalo; Peña-Monné, José Luis; Rhodes, Edward J.; Bartolomé, Miguel; García-Ruiz, José M.; Martí-Bono, Carlos

    2018-04-01

    Combined geomorphic features, stratigraphic characteristics and sedimentologic interpretation, coupled with optically stimulated luminescence (OSL) dates, of a glacio-fluvio-lacustrine sequence (Linás de Broto, northern Spain) provide new information to understand the palaeoenvironmental significance of dynamics of glacier systems in the south-central Pyrenees during the Last Glacial Cycle (≈130 ka to 14 ka). The Linás de Broto depositional system consisted of a proglacial lake fed primarily by meltwater streams emanating from the small Sorrosal glacier and dammed by a lateral moraine of the Ara trunk glacier. The resulting glacio-fluvio-lacustrine sequence, around 55 m thick, is divided into five lithological units consisting of braided fluvial (gravel deposits), lake margin (gravel and sand deltaic deposits) and distal lake (silt and clay laminites) facies associations. Evolution of the depositional environment reflects three phases of progradation of a high-energy braided fluvial system separated by two phases of rapid expansion of the lake. Fluvial progradation occurred during short periods of ice melting. Lake expansion concurred with ice-dam growth of the trunk glacier. The first lake expansion occurred over a time range between 55 ± 9 ka and 49 ± 11 ka, and is consistent with the age of the Viu lateral moraine (49 ± 8 ka), which marks the maximum areal extent of the Ara glacier during the Last Glacial Cycle. These dates confirm that the maximum areal extent of the glacier occurred during Marine Isotope Stages 4 and 3 in the south-central Pyrenees, thus before the Last Glacial Maximum. The evolution of the Linás de Broto depositional system during this maximum glacier extent was modulated by climate oscillations in the northern Iberian Peninsula, probably related to latitudinal shifts of the atmospheric circulation in the southern North-Atlantic Ocean, and variations in summer insolation intensity.

  9. Geological map of the Paleozoic of the Central Pyrenees

    NARCIS (Netherlands)

    Sitter, de L.U.; Zwart, H.J.

    1957-01-01

    Mapping of the Paleozoic of the Central Pyrenees by students in geology of Leiden University under the directorship of Prof. Dr L.U. de Sitter started in 1948 with the Arize massif. Since then the survey has shifted first eastwards, 1949 St. Barthélemy massif; then westwards, 1950 Salat valley; 1951

  10. Ethnobotany of food plants in the high river Ter valley (Pyrenees, Catalonia, Iberian Peninsula): non-crop food vascular plants and crop food plants with medicinal properties.

    Science.gov (United States)

    Rigat, Montse; Bonet, Maria Àngels; Garcia, Sònia; Garnatje, Teresa; Vallès, Joan

    2009-01-01

    The present study reports a part of the findings of an ethnobotanical research project conducted in the Catalan region of the high river Ter valley (Iberian Peninsula), concerning the use of wild vascular plants as food and the medicinal uses of both wild and cultivated food plants. We have detected 100 species which are or have been consumed in this region, 83 of which are treated here (the remaining are the cultivated food plants without additional medicinal uses). Some of them, such as Achillea ptarmica subsp. pyrenaica, Convolvulus arvensis, Leontodon hispidus, Molopospermum peloponnesiacum and Taraxacum dissectum, have not been previously reported, or have only very rarely been cited or indicated as plant foods in very restricted geographical areas. Several of these edible wild plants have a therapeutic use attributed to them by local people, making them a kind of functional food. They are usually eaten raw, dressed in salads or cooked; the elaboration of products from these species such as liquors or marmalades is a common practice in the region. The consumption of these resources is still fairly alive in popular practice, as is the existence of homegardens, where many of these plants are cultivated for private consumption.

  11. ASP made simple

    CERN Document Server

    Deane, Sharon

    2003-01-01

    ASP Made Simple provides a brief introduction to ASP for the person who favours self teaching and/or does not have expensive computing facilities to learn on. The book will demonstrate how the principles of ASP can be learned with an ordinary PC running Personal Web Server, MS Access and a general text editor like Notepad.After working through the material readers should be able to:* Write ASP scripts that can display changing information on a web browser* Request records from a remote database or add records to it* Check user names & passwords and take this knowledge forward, either for their

  12. QuickASP: PEMBANGKIT KODE PROGRAM ASP UNTUK APLIKASI BASIS DATA BERBASIS WEB

    Directory of Open Access Journals (Sweden)

    Imam Kuswardayan

    2007-01-01

    Full Text Available Dalam pembuatan sistem aplikasi basis data berbasis web, perancangan antarmuka pengguna (presentation layer dan lapisan bisnis (bussiness layer merupakan tahap yang dilalui setelah pemahaman terhadap kebutuhan pengguna sistem. Adanya pola atau keteraturan dalam implementasi tahap ini menyebabkan pengembangan sistem akan lebih efisien jika menggunakan suatu aplikasi yang dapat menghasilkan kerangka dasar aplikasi web dengan cepat untuk kedua lapisan tersebut dan bahkan beserta kode programnya. Pada penelitian ini telah diimplementasikan suatu perangkat lunak yang selanjutnya disebut QuickASP. QuickASP membangkitkan kode ASP untuk membangun  homepage otomatis. Untuk membangkitkan kode ASP, QuickASP membutuhkan komponen berupa basis data dan file Cascading Style Sheets (CSS. Proses awal yang dilakukan QuickASP dalam membangkitkan kode program ASP adalah membaca informasi basis data berupa tabel-tabel, nama field dan tipe data. Setelah itu QuickASP akan membangkitkan file-file ASP beserta file-file pendukungnya berdasarkan hasil pengaturan tampilan halaman web yang dilakukan oleh pengguna.Uji coba QuickASP dilakukan pada tiga jenis basis data yaitu Microsoft Access, Microsoft SQL Server, dan Oracle. Dari hasil uji coba tersebut, QuickASP terbukti dapat membangkitkan homepage otomatis beserta fungsi–fungsi yang disediakan untuk modifikasi record dan fungsi navigasi.Kata kunci: QuickASP, file cascading style sheets, kode program ASP.

  13. Pro ASP.NET MVC 4

    CERN Document Server

    Freeman, Adam

    2012-01-01

    The ASP.NET MVC 4 Framework is the latest evolution of Microsoft's ASP.NET web platform. It provides a high-productivity programming model that promotes cleaner code architecture, test-driven development, and powerful extensibility, combined with all the benefits of ASP.NET. ASP.NET MVC 4 contains a number of significant advances over previous versions. New mobile and desktop templates (employing adaptive rendering) are included together with support for jQuery Mobile for the first time. New display modes allow your application to select views based on the browser that's making the request whi

  14. Influence of spring snowpack melting on thunderstorm activity in the Catalan Pyrenees

    Science.gov (United States)

    Pascual, R.; Callado, A.; Terradelles, E.; Téllez, B.

    2009-09-01

    Catalan Pyrenees, the eastern half of the Pyrenees range, is a very popular area for tourism, hiking and climbing. This sector of the range is 200 km long and, on average, 80 km wide. Its highest peaks reach 3000 m ASL and there are many summits above 2500 m ASL. Two of the main climatic characteristics of the region are the very frequent summer convective storms and the late autumn, winter and spring snow-cover. Both characteristics have normally been studied from different points of view, and weather forecasts in late spring have not normally considered the plausible relationship between them. The snowpack melting from April to June, especially rapid in May, leads to important changes on the surface energy balance since the evolution from snow-covered ground to bare soil or canopy, significantly alters the surface albedo and the turbulent, latent and sensible, heat fluxes. These modifications have a noticeable influence in developing or inhibiting thermally-induced mesoscale circulations such as upslope winds, valley breezes or plane-mountain breezes, and could condition the triggering of convection, showers and storm activity. In order to gain insight into the relationship between the spring snowpack melting and the location of thunderstorm activity, a comparison between seasonal snow-cover and thunderstorm frequency evolution (using lightning network data) for a period of 5 years has been carried out, showing a progressive transition from a non-convective to a convective precipitation regime in areas where the snowpack has melted recently Furthermore, a meso-beta scale non-hydrostatic numerical weather prediction model at a 2.5-km horizontal resolution is used to study the sensitivity of snowpack extension on the thunderstorms development over the complex orography of the Catalan Pyrenees. A spring case with thunderstorm activity restricted to snow-free areas has been selected and accurately simulated. A number of sensitivity runs with different initial snow

  15. Complex climate constraints of upper treeline formation in the Pyrenees

    Czech Academy of Sciences Publication Activity Database

    Gonzalez de Andres, E.; Julio Camarero, J.; Büntgen, Ulf

    2015-01-01

    Roč. 29, č. 3 (2015), s. 941-952 ISSN 0931-1890 Institutional support: RVO:67179843 Keywords : pinus-uncinata * spanish pyrenees * summer temperature * sierra-nevada * wood density * ring growth * variability * altitude * mountain * dynamics * Basal area increment * Drought stress * Mediterranean * Pinus uncinata * Temperature * Treeline Subject RIV: EH - Ecology, Behaviour Impact factor: 1.706, year: 2015

  16. Modelling regional land change scenarios to assess land abandonment and reforestation dynamics in the Pyrenees (France)

    Science.gov (United States)

    Vacquie, Laure; Houet, Thomas; Sohl, Terry L.; Reker, Ryan R.; Sayler, Kristi L.

    2015-01-01

    Over the last decades and centuries, European mountain landscapes have experienced substantial transformations. Natural and anthropogenic LULC changes (land use and land cover changes), especially agro-pastoral activities, have directly influenced the spatial organization and composition of European mountain landscapes. For the past sixty years, natural reforestation has been occurring due to a decline in both agricultural production activities and rural population. Stakeholders, to better anticipate future changes, need spatially and temporally explicit models to identify areas at risk of land change and possible abandonment. This paper presents an integrated approach combining forecasting scenarios and a LULC changes simulation model to assess where LULC changes may occur in the Pyrenees Mountains, based on historical LULC trends and a range of future socio-economic drivers. The proposed methodology considers local specificities of the Pyrenean valleys, sub-regional climate and topographical properties, and regional economic policies. Results indicate that some regions are projected to face strong abandonment, regardless of the scenario conditions. Overall, high rates of change are associated with administrative regions where land productivity is highly dependent on socio-economic drivers and climatic and environmental conditions limit intensive (agricultural and/or pastoral) production and profitability. The combination of the results for the four scenarios allows assessments of where encroachment (e.g. colonization by shrublands) and reforestation are the most probable. This assessment intends to provide insight into the potential future development of the Pyrenees to help identify areas that are the most sensitive to change and to guide decision makers to help their management decisions.

  17. First comprehensive contribution to medical ethnobotany of Western Pyrenees.

    Science.gov (United States)

    Akerreta, Silvia; Cavero, Rita Yolanda; Calvo, María Isabel

    2007-06-06

    An ethnobotanical and medical study was carried out in the Navarre Pyrenees, an area known both for its high biological diversity and its cultural significance. As well as the compilation of an ethnopharmacological catalogue, a quantitative ethnobotanical comparison has been carried out in relation to the outcomes from other studies about the Pyrenees. A review of all drugs used in the area has also been carried out, through a study of the monographs published by the institutions and organizations responsible for the safety and efficacy of medicinal plants (WHO, ESCOP, and the E Commission of the German Department of Health) in order to ascertain the extent to which the Navarre Pyrenees ethnopharmacology has been officially evaluated. Fieldwork was carried out over two years, from November 2004 to December 2006. During that time we interviewed 88 local people in 40 villages. Information was collected using semi-structured ethnobotanical interviews and the data was analyzed using quantitative indexes: Ethnobotonicity Index, Shannon-Wiener's Diversity, Equitability and The Informant Consensus Factor. The official review has been performed using the official monographs published by the WHO, ESCOP and the E Commission of the German Department of Health. The ethnobotanical and medical catalogue of the Navarre Pyrenees Area comprises 92 species, of which 39 have been mentioned by at least three interviewees. The quantitative ethnobotany results show lower values than those found in other studies about the Pyrenees; and 57.6% of the Pyrenees medical ethnobotany described does not figure in documents published by the above mentioned institutions. The results show a reduction in the ethnobotanical and medical knowledge in the area of study, when compared to other studies carried out in the Pyrenees. Nevertheless, the use of several species that may be regarded as possible sources for pharmacological studies is reported here such as the bark of Sambucus nigra, the roots of

  18. Pro ASP.NET 4 in VB 2010

    CERN Document Server

    MacDonald, Matthew; Freeman, Adam; Szpuszta, Mario; Agarwal, Vidya Vrat

    2010-01-01

    ASP.NET 4 is the latest version of Microsoft's revolutionary ASP.NET technology. It is the principal standard for creating dynamic web pages on the Windows platform. Pro ASP.NET 4 in VB 2010 raises the bar for high-quality, practical advice on learning and deploying Microsoft's dynamic web solution. This new edition is updated with everything you need to come to grips with the latest version of ASP.NET, including coverage of ASP.NET MVC, ASP.NET AJAX 4, ASP.NET Dynamic Data, and Silverlight 3. Seasoned .NET professionals Matthew MacDonald and Mario Szpuszta explain how you can get the most fro

  19. The diffusion of cultural heritage through ICT: the case of the Boí valley portal

    Directory of Open Access Journals (Sweden)

    Piero Berni Millet

    2003-04-01

    Full Text Available The Boí valley portal is a joint project involving different museums, institutions and universities in Catalonia with the aim of creating a virtual environment to include all of the cultural heritage of this small valley in the Pyrenees. The portal is not only a potential resource for cultural tourism, but also an art and history resource for secondary schools and universities, as well as a means of communication for the local community.

  20. A Compared Aproach: ASP versus PHP

    Directory of Open Access Journals (Sweden)

    Liana-Maria STANCA

    2006-01-01

    Full Text Available In the development process of electronic business theory, we attributed a very important role to technologies of business projection on the Internet. Starting with this idea, in the following article we will compare the most used technologies in the development of electronic business application like ASP and PHP.

  1. Saphire models and software for ASP evaluations

    International Nuclear Information System (INIS)

    Sattison, M.B.

    1997-01-01

    The Idaho National Engineering Laboratory (INEL) over the three years has created 75 plant-specific Accident Sequence Precursor (ASP) models using the SAPHIRE suite of PRA codes. Along with the new models, the INEL has also developed a new module for SAPHIRE which is tailored specifically to the unique needs of ASP evaluations. These models and software will be the next generation of risk tools for the evaluation of accident precursors by both the U.S. Nuclear Regulatory Commission's (NRC's) Office of Nuclear Reactor Regulation (NRR) and the Office for Analysis and Evaluation of Operational Data (AEOD). This paper presents an overview of the models and software. Key characteristics include: (1) classification of the plant models according to plant response with a unique set of event trees for each plant class, (2) plant-specific fault trees using supercomponents, (3) generation and retention of all system and sequence cutsets, (4) full flexibility in modifying logic, regenerating cutsets, and requantifying results, and (5) user interface for streamlined evaluation of ASP events. Future plans for the ASP models is also presented

  2. Adventure sports and tourism at the beginning of the construction of Europe in the Pyrenees

    Directory of Open Access Journals (Sweden)

    André Suchet

    2011-12-01

    Full Text Available Organised in the spring of 1993 on the border between France and Spain, the Pyrenees Adventure Games (les Jeux Pyrénéens de l’Aventure or los Juegos Pirenaicos de la Aventura, brought together more than 1,000 athletes from 26 countries and were attended by 21,000 visitors. An alternative Olympics of adventure and outdoor sports patronised by the IOC, the events took place in the Aure Valley in France (Hautes-Pyrénées and in the Sobrarbe in Spain (Huesca province. This article analyses the governance of this international sports competition. More specifically, this case study will enable us to find out more about the conditions of organisation of a cross-border project in the early 1990s. What were the various political, social and cultural exchanges between the valleys in terms of sports and tourism in the Pyrenees? The results show that the French organisation and the Spanish organisation functioned side by side instead of working together on the field. This division in working enabled them to bypass the language problem. The cross-border dimension of these adventure Olympics in 1993 stemmed from pressure from the French regional planning agency, DATAR right from the beginning of the project, i.e. in October 1989, to be exact. This dimension made the 1993 Pyrenees Adventure Games a testimony to the new political Europe that was being built after 1989. This period was marked by the proactiveness of institutions, which far exceeded the motivations of the populations of the Union.Organisés au printemps 1993 sur la frontière entre la France et l’Espagne, les Jeux Pyrénéens de l’Aventure, ou los Juegos Pirenaicos de la Aventura, réunirent plus de 1 000 athlètes venus de 26 pays du monde sous les yeux de 21 000 visiteurs. Olympiade des sports d’aventure et de nature patronnée par le CIO, les épreuves se déroulèrent dans la vallée d’Aure en France (département des Hautes-Pyrénées et le Sobrarbe en Espagne (province de

  3. Elizabeth Croll: ASPS Patient of Courage 2016.

    Science.gov (United States)

    Gosain, Arun K

    2017-07-01

    Mrs. Elizabeth Croll was selected as an American Society of Plastic Surgeons (ASPS) Patient of Courage in 2016. During a boating accident that occurred within 1 month after graduating from Harvard Business School, she was struck by an oncoming boat and suffered severe facial trauma, resulting in an open skull injury with loss of her right eye. She has since undergone multiple reconstructive surgical procedures beginning in 1997 and continuing up to the month before she was awarded the ASPS Patient of Courage. During this time, she got married, started a family, and continued an ambitious business career. Mrs. Croll is the epitome of an individual who has overcome tragedy to further her career, her community, and access to medical care for indigent children.

  4. Professional ASP.NET MVC 2

    CERN Document Server

    Galloway, Jon; Haack, Phil

    2010-01-01

    Top-selling MVC book from a top team at Microsoft—now fully updated!. ASP.NET MVC 2.0 is now available and shipping with Visual Studio 2010 and .NET 4. A new update to Microsoft's Model-View-Controller technologies, MVC 2.0 enables developers to build dynamic, data-driven Web sites. This in-depth book shows you step-by-step how to use MVC 2.0. You'll learn both the theory behind MVC 2.0, as well as walk through practical tutorials, where you'll create a real-world application. Topics include transitioning from ASP.NET development, as well as an overview of related tools and technologies, inclu

  5. External controls on Quaternary fluvial incision and terrace formation at the Segre River, Southern Pyrenees

    NARCIS (Netherlands)

    Stange, K.M.; van Balen, R.T.; Vandenberghe, J.; Peña, J.L.; Sancho, C.

    2013-01-01

    Focusing on climatic- and structural (tectonic) controls, we aim to determine their relative importance for the (Pliocene to Quaternary) fluvial landscape evolution in the Southern Pyrenees foreland. We investigate the Segre River, which is one of the major streams of the Southern Pyrenees that

  6. Diagnosis and modelling of a summer convective storm over Mediterranean Pyrenees

    Directory of Open Access Journals (Sweden)

    A. Callado

    2005-01-01

    Full Text Available Over the Mediterranean Pyrenees and near coastal region there is a high frequency of convective storms during summertime. This fact determines a local maximum of precipitation at this season unlike the normal and general minimum over Mediterranean basin. The common synoptic and mesoscale patterns associated to convective phenomena are briefly analyzed and diagnosed with radar imagery, surface pressure and isobaric level fields and surface data. They usually involves a little distant through at medium levels, very weak pressure gradient at the surface and not significant instability, but a preferred Planetary Boundary Layer low-level convergence zones tied to the orography. As a result of this analysis two different kinds of circulations associated to recurrent convergence zones have been recognized: upvalley/upslope winds and sea breezes. High resolution simulations from a Numerical Weather Prediction model for a case study have carried out to analyze the role of the main key components, i.e. the orography, the sea and valley breeze and their influence to generate local convergence zones. Furthermore, the skills of the model to simulate the location and the intensity of the precipitation of these summer Mediterranean storms have been discussed.

  7. Pro ASP.NET 4 CMS

    CERN Document Server

    Harris, Alan

    2010-01-01

    To be a successful ASP.NET 4 developer, you need to know how to apply the vast array of new functionality available in the latest release of the .NET 4 Framework and Visual Studio 2010. This book will immerse you in a variety of advanced topics, including architecting different application data tiers, memory caching paradigms, data mining, and search engine optimization. Working through step-by-step exercises using P/LINQ, DLR, MEF, MVC, IronPython, Axum, and Ajax, you will learn a variety of approaches to building each of the key application tiers common to all web solutions. Using a proven t

  8. Electronic trigger for the ASP experiment

    International Nuclear Information System (INIS)

    Wilson, R.J.

    1985-11-01

    The Anomalous Single Photon (ASP) electronic trigger is described. The experiments is based on an electromagnetic calorimeter composed of arrays of lead glass blocks, read out with photo-multiplier tubes, surrounding the interaction point at the PEP storage ring. The primary requirement of the trigger system is to be sensitive to low energy (approx. =0.5 GeV and above) photons whilst discriminating against high backgrounds at PEP. Analogue summing of the PMT signals and a sequence of programmable digital look-up tables produces a ''dead-timeless'' trigger for the beam collision rate of 408 kHz. 6 refs., 6 figs

  9. Electronic trigger for the ASP experiment

    International Nuclear Information System (INIS)

    Wilson, R.J.

    1985-01-01

    The ASP electronic trigger is described. The experiment is based on an electromagnetic calorimeter composed of arrays of lead glass blocks, read out with photo-multiplier tubes, surrounding the interaction point at the PEP storage ring. The primary requirement of the trigger system is to be sensitive to low energy (≅ 0.5 GeV and above) photons whilst discriminating against high rate backgrounds at PEP. Analogue summing of the PMT signals and a sequence of programmable digital look-up tables produces a ''dead-timeless'' trigger for the beam collision rate of 408 kHz

  10. The Midi-Pyrenees regional energy observatory - Release 2005

    International Nuclear Information System (INIS)

    Malvy, Martin; Daubigny, Jean; Fraysse, Jean-Marie; Dedieu-Casties, Francoise; RIEY, Benedicte

    2005-01-01

    Illustrated with maps and graphs, this publication proposes a synthetic and brief energy assessment for the Midi-Pyrenees region. It briefly presents the regional energy situation in terms of final energy consumption between 1990 and 2003, of primary energy production during the same period, of inventories of greenhouse gas emissions, and of CO 2 emissions by the energy sector. It also proposes an overview of the situation, evolution and production of various energy sources: hydroelectricity, wood, wind, solar photovoltaic, solar and thermal energy, co-generation, and other types of energy valorisation modes (biogas, combustion). It proposes a focus on two important sectors, transports and housing

  11. Shifting agriculture: the main cause of landscape degradation in the Central Spanish Pyrenees

    Science.gov (United States)

    Lasanta, Teodoro; Nadal-Romero, Estela; Errea, Paz

    2017-04-01

    Cereal agriculture occupied large areas in the Spanish Pyrenees to feed the population in a socio-economic system of limited exchanges with the outside. In the Western valleys, shifting agriculture constitutes the dominant field pattern, representing almost three-quarters of the traditional agricultural space (Lasanta et al., in press). These were cultivated at times of heavy population growth, necessitating steep and stony hillsides with poor soil to be tilled, or the ones that were far away from the village. The fields were created by clearing the vegetation from a slope, then burning it to use the ash as a fertilizer. Cereal was grown for 3-4 years, after which they were abandoned for 20-30 years to recover fertility, and the cycle was repeated. Almost all the fields (99%) using shifting agriculture had been abandoned by the 1950s. This study analyzes the role of the shifting agriculture in soil erosion and landscape degradation. For this purpose, (i) experimental plots, which reproduce the traditional agriculture in the Pyrenees and the abandonment processes, and (ii) the cartography made from the SIOSE (2009), which shows the present land cover 50 years after cropland abandonment, were used. The results show that shifting agriculture caused higher soil losses than other agricultural uses (1.36 kg m-2 yr-1): fallow land (0.87 kg m-2 yr-1), chemically fertilized cereal (0.86 kg m-2 yr-1) and meadow (0.14 kg m-2 yr-1). Also, after land abandonment, soil losses are higher in shifting agriculture (0.78 kg m-2 yr-1) than cereal lands (0.73 kg m-2 yr-1). The burning of the shrub cover and the use of ashes as fertilizer did not contribute to improve the soil quality, which explains both the higher soil losses during the cultivated period and after the abandonment, since slower plant succession occurs. The results obtained from the SIOSE confirm that the change from meadows to shrubland is relatively fast, as a consequence of the low relationship with livestock

  12. Filamentous fungal-specific septin AspE is phosphorylated in vivo and interacts with actin, tubulin and other septins in the human pathogen Aspergillus fumigatus

    Energy Technology Data Exchange (ETDEWEB)

    Juvvadi, Praveen Rao; Belina, Detti [Division of Pediatric Infectious Diseases, Department of Pediatrics, Duke University Medical Center, Durham, NC (United States); Soderblom, Erik J.; Moseley, M. Arthur [Duke Proteomics Core Facility, Institute for Genome Sciences and Policy, Duke University, Durham, NC (United States); Steinbach, William J., E-mail: bill.steinbach@duke.edu [Division of Pediatric Infectious Diseases, Department of Pediatrics, Duke University Medical Center, Durham, NC (United States); Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC (United States)

    2013-02-15

    Highlights: ► In vivo interactions of the novel septin AspE were identified by GFP-Trap® affinity purification. ► Septins AspA, AspB, AspC and AspD interacted with AspE in vivo. ► Actin and tubulin interacted with AspE in vivo. ► AspE is phosphorylated at six serine residues in vivo. -- Abstract: We previously analyzed the differential localization patterns of five septins (AspA–E), including a filamentous fungal-specific septin, AspE, in the human pathogen Aspergillus fumigatus. Here we utilized the A. fumigatus strain expressing an AspE–EGFP fusion protein and show that this novel septin with a tubular localization pattern in hyphae is phosphorylated in vivo and interacts with the other septins, AspA, AspB, AspC and AspD. The other major proteins interacting with AspE included the cytoskeletal proteins, actin and tubulin, which may be involved in the organization and transport of the septins. This is the first report analyzing the phosphorylation of AspE and localizing the sites of phosphorylation, and opens opportunities for further analysis on the role of post-translational modifications in the assembly and organization of A. fumigatus septins. This study also describes the previously unknown interaction of AspE with the actin-microtubule network. Furthermore, the novel GFP-Trap® affinity purification method used here complements widely-used GFP localization studies in fungal systems.

  13. The Pyrenees earthquake of 1660: Effects in France.

    Science.gov (United States)

    Vogt, J

    1983-09-01

    Until 1975 or so, scientists' knowledge of the major earthquake of 1660 in the Pyrenees (in Bigorre) was largely restricted to the meagre information repeated in the classical catalogues. Useful material published in local learned journals was mostly unknown, and has only been unearthed and interpreted since 1976, when two year's work of analysis was started for the "Projet Sismo-tectonique" in France. During and since that project, further routine work necessary for the revision of the historical seismicity of France, has brought to light a new wealth of information from archive sources. This new knowledge, at least from the French side, is used as the basis for a brief macroseismic review of the event north of the Pyrenees, putting forward proposals for the isoseismal lines. Emphasis is placed on the number and quality of the sources, some of which are quoted for evidence of major rockfalls in the meizoseismal region and the wide extent of the felt area, as far north as Salnt-Maixent in Poitou.

  14. Valley Fever

    Science.gov (United States)

    Valley Fever is a disease caused by a fungus (or mold) called Coccidioides. The fungi live in the soil ... from person to person. Anyone can get Valley Fever. But it's most common among older adults, especially ...

  15. A multi-proxy perspective on millennium-long climate variability in the Southern Pyrenees

    NARCIS (Netherlands)

    Morellón, M.; Pérez-Sanz, A.; Corella, J.P.; Büntgen, U.; Catalán, J.; González-Samprizé, P.; González-Trueba, J.J.; López-Sáez, J.A.; Moreno, A.; Pla-Rabes, S.; Saz-Sánchez, M.Á.; Scussolini, P.; Serrano, E.; Steinhilber, F.; Stefanova, V.; Vegas-Vilarrúbia, T.; Valero-Garcés, B.

    2012-01-01

    This paper reviews multi-proxy paleoclimatic reconstructions with robust age-control derived from lacustrine, dendrochronological and geomorphological records and characterizes the main environmental changes that occurred in the Southern Pyrenees during the last millennium. Warmer and relatively

  16. Microsatellite marker analysis reveals the complex phylogeographic history of Rhododendron ferrugineum (Ericaceae in the Pyrenees.

    Directory of Open Access Journals (Sweden)

    Olivia Charrier

    Full Text Available Genetic variation within plant species is determined by a number of factors such as reproductive mode, breeding system, life history traits and climatic events. In alpine regions, plants experience heterogenic abiotic conditions that influence the population's genetic structure. The aim of this study was to investigate the genetic structure and phylogeographic history of the subalpine shrub Rhododendron ferrugineum across the Pyrenees and the links between the populations in the Pyrenees, the Alps and Jura Mountains. We used 27 microsatellite markers to genotype 645 samples from 29 Pyrenean populations, three from the Alps and one from the Jura Mountains. These data were used to estimate population genetics statistics such as allelic richness, observed heterozygosity, expected heterozygosity, fixation index, inbreeding coefficient and number of migrants. Genetic diversity was found to be higher in the Alps than in the Pyrenees suggesting colonization waves from the Alps to the Pyrenees. Two separate genetic lineages were found in both the Alps and Pyrenees, with a substructure of five genetic clusters in the Pyrenees where a loss of genetic diversity was noted. The strong differentiation among clusters is maintained by low gene flow across populations. Moreover, some populations showed higher genetic diversity than others and presented rare alleles that may indicate the presence of alpine refugia. Two lineages of R. ferrugineum have colonized the Pyrenees from the Alps. Then, during glaciation events R. ferrugineum survived in the Pyrenees in different refugia such as lowland refugia at the eastern part of the chain and nunataks at high elevations leading to a clustered genetic pattern.

  17. Fluvial-system response to climate change: The Paleocene-Eocene Tremp Group, Pyrenees, Spain

    Science.gov (United States)

    Colombera, Luca; Arévalo, Oscar J.; Mountney, Nigel P.

    2017-10-01

    The Tremp Group of the Tremp-Graus Basin (Southern Pyrenees, Spain) is a succession of predominantly continental origin that records the Paleocene-Eocene Thermal Maximum (PETM), a transient episode of extreme global warming that occurred across the Paleocene-Eocene boundary. For this succession, the stratigraphic position of the PETM is accurately determined, and histories of tectonic and sea-level controls are well constrained. Building upon previous studies, this work assesses changes in sedimentary architecture through the PETM in the Tremp Group, based on quantitative sedimentological analyses documented over a km-scale strike-oriented transect in the Arén area, with the scope to better understand the response of this alluvial system to the hyperthermal event. The analysed features represent a partial record of the geomorphic organization and processes of the system at the time of deposition, and are therefore interpretable in terms of geomorphic change in alluvial landscapes caused by the PETM. The record of the PETM, as previously recognized, begins at a time when erosional palaeotopographic relief was developed and deposition was confined in valleys. A shift between valley back-filling and widespread aggradation is observed at the onset of the PETM interval, which demonstrates uniquely the impact of the hyperthermal on both depositional loci and interfluves. Compared to underlying strata, the interval that embodies the onset and main phase of the PETM is characterized by: (i) higher proportion of channel deposits; (ii) channel complexes of greater average thickness and width; (iii) barforms and channel fills that are slightly thicker; (iv) increased thickness of sets of cross-stratified sandstones; (v) similar values of maximum extraclast size, by architectural element. An evident change in the facies organization of channel deposits is also seen through the stratigraphy, though this appears to predate the PETM. Increased channel-body density in the PETM

  18. The geology of the Upper Ribagorzana and Baliera valleys, Central Pyrenees, Spain

    NARCIS (Netherlands)

    Mey, P.H.W.

    1968-01-01

    In the mapped area there is a well-exposed low-grade metamorphic marine sequence from Ordovician to Lower Carboniferous, unconformably overlain by Permo-Triassic continental deposits. Determinable fossils are rare. The Ordovician consists of a quartzite/shale sequence with one marly limestone

  19. The Road Network of the Catalan Pyrenees: Development and Current State

    Directory of Open Access Journals (Sweden)

    Jaume Font

    2012-10-01

    Full Text Available In this article we analyse the process of development andthe functional characteristics of the road network in the CatalanPyrenees. The study is divided in three main sections: 1 ananalysis of the geographical factors that have been determinantin the growth of the network; 2 a description of the process ofits regional implementation and 3 its current state, includingan evaluation of the plans now in the process of being implementedand recent improvements and an analysis of the significanceof two major undertakings - the Llobregat Axis and thetunnel of Cadi. Special emphasis is placed throughout the wticleon the concept of network integration Labasse, J. 1971,which we define as the degree of connectivity of the comarcas(provincial subdivisions of the Catalan Pyrenees and theircentres of population to a general road network.This mticle has drawn on both documentmy and bibliographicsources but is based principally on J. Font's doctoralthesis, which analyses the growth of the Catalan road network.Few geographical studies of the transpmt network of the southemslopes of the Pyrenees have been published, be it in Catalanor Spanish journals. This stands in marked contrast to the attentiondedicated to the subject by French academic publications(Pyrenees, Revue de Geograhie des Pyrenees et duSud-Ouest. This said, howevet; we should not overlook thestudies undettaken in Spain by A. Escalona ( 1888, above allthose carried out in the A rag on Pyrenees. Any other articles describingthe Catalan Pyrenees, though, must be tracked down inindividual chapters of regional monographs, both ancient(Sole Sabaris, L. 1951; Llobet, S., 1947 and modem (L6pezPalomeque, F., 1986.The data sources desctibing the Pyrenees transport networkhave improved considerably in recent years. This is due to thegrowth in number of applied studies sponsored by the variousbodies of public administration following the re-establishmentof democracy in Spain (1977 and the restoration of the

  20. ASP.NET MVC 4 mobile app development

    CERN Document Server

    Meadows, Andy

    2013-01-01

    A step-by-step tutorial to get acquainted with the ASP.NET MVC4 Framework and its features in order to discover how to develop web applications using them.This book is targeted at people who are familiar with C# development on the .NET platform and are interested in web development with the ASP.NET development framework. No prior web or mobile development experience is required

  1. From plot to regional scales: Interactions of slope and catchment hydrological and geomorphic processes in the Spanish Pyrenees

    Science.gov (United States)

    García-Ruiz, José M.; Lana-Renault, Noemí; Beguería, Santiago; Lasanta, Teodoro; Regüés, David; Nadal-Romero, Estela; Serrano-Muela, Pilar; López-Moreno, Juan I.; Alvera, Bernardo; Martí-Bono, Carlos; Alatorre, Luis C.

    2010-08-01

    The hydrological and geomorphic effects of land use/land cover changes, particularly those associated with vegetation regrowth after farmland abandonment were investigated in the Central Spanish Pyrenees. The main focus was to assess the interactions among slope, catchment, basin, and fluvial channel processes over a range of spatial scales. In recent centuries most Mediterranean mountain areas have been subjected to significant human pressure through deforestation, cultivation of steep slopes, fires, and overgrazing. Depopulation commencing at the beginning of the 20th century, and particularly since the 1960s, has resulted in farmland abandonment and a reduction in livestock numbers, and this has led to an expansion of shrubs and forests. Studies in the Central Spanish Pyrenees, based on experimental plots and catchments, in large basins and fluvial channels, have confirmed that these land use changes have had hydrological and geomorphic consequences regardless of the spatial scale considered, and that processes occurring at any particular scale can be explained by such processes acting on other scales. Studies using experimental plots have demonstrated that during the period of greatest human pressure (mainly the 18th and 19th centuries), cultivation of steep slopes caused high runoff rates and extreme soil loss. Large parts of the small catchments behaved as runoff and sediment source areas, whereas the fluvial channels of large basins showed signs of high torrentiality (braided morphology, bare sedimentary bars, instability, and prevalence of bedload transport). Depopulation has concentrated most human pressure on the valley bottoms and specific locations such as resorts, whereas the remainder of the area has been affected by an almost generalized abandonment. Subsequent plant recolonization has resulted in a reduction of overland flow and declining soil erosion. At a catchment scale this has caused a reduction in sediment sources, and channel incision in the

  2. Valley Fever

    Science.gov (United States)

    ... loss Headache Valley fever Symptoms & causes Diagnosis & treatment Advertisement Mayo Clinic does not endorse companies or products. ... a Job Site Map About This Site Twitter Facebook Google YouTube Pinterest Mayo Clinic is a not- ...

  3. Basque Cultural Landscapes of the Western French Pyrenees

    Directory of Open Access Journals (Sweden)

    Ted L Gragson

    2015-12-01

    Full Text Available Results are presented on the co-evolution of agropastoralism and soils in the western Pyrenees Mountains (>800 masl over the course of the Holocene conducted in the ethnically Basque commune of Larrau, France. Larrau presents a unique opportunity to examine the structural legacies and biotic factor in soil evolution across millennia. Multi-proxy evidence from geoarchives, archaeology, history and ethnography is analyzed to evaluate the relation between land management practices, soil characteristics and chronostratigraphy in the study area. Research indicates that the landscape of Larrau has been subject to intense human transformation through agropastoral use since at least the early Bronze age, yet there are no signs of significant degradation of the soil mantle. The place-based approach followed in this research provides the means for evaluating modal human behaviors and decisionmaking within a complex adaptive system. It details how the present is connected to the past and how contemporary land systems can contribute to a sustainable future. Nel corso della ricerca effettuata nel comune etnicamente basco di Larrau (Francia abbiamo indagato tracce multi-proxy per esaminare le forme di domesticazione dei paesaggi montani (> 800 m slm dei Pirenei occidentali, nel corso dell’Olocene. I nostri dati suggeriscono che in quest’area le foreste originarie sono state trasformate in pascoli diverse migliaia di anni fa, senza che questo abbia comportato un degrado significativo del paesaggio e dei suoli. Il patrimonio dei paesaggi agropastorali di Larrau offre una rara opportunità per esaminare un doppio sistema in lento cambiamento, nel quale, attraverso millenni, le attività di gestione umana hanno strutturato un paesaggio scenografico, creando un sistema di produzione agropastorale resiliente e durevole, e hanno reindirizzato i sottostanti percorsi e meccanismi di pedogenesi. Se i sistemi terrestri contemporanei mirano a raggiungere un

  4. Beginning ASP.NET 4.5 in VB

    CERN Document Server

    MacDonald, Matthew

    2012-01-01

    This book is the most comprehensive and up to date introduction to ASP.NET ever written. Focusing solely on Visual Basic, with no code samples duplicated in other languages, award winning author Matthew MacDonald introduces you to the very latest thinking and best practices for the ASP.NET 4.5 technology.Assuming no prior coding experience, you'll be taught everything you need to know from the ground up.  Starting from first principals, you'll learn the skills you need to be an effective ASP.NET developer who is ready to progress to more sophisticated projects and professional work.You'll be t

  5. Beginning ASP.NET 4.5 in C#

    CERN Document Server

    MacDonald, Matthew

    2012-01-01

    This book is the most comprehensive and up to date introduction to ASP.NET ever written. Focussing solely on C#, with no code samples duplicated in other languages, award winning author Matthew MacDonald introduces you to the very latest thinking and best practices for the ASP.NET 4.5 technology.Assuming no prior coding experience, you'll be taught everything you need to know from the ground up.  Starting from first principals, you'll learn the skills you need to be an effective ASP.NET developer who is ready to progress to more sophisticated projects and professional work. You'll be taught ho

  6. Review of Clinical Profile of IDegAsp.

    Science.gov (United States)

    Unnikrishnan, A G; Singh, Awadhesh Kumar; Modi, K D; Saboo, Banshi; Garcha, Shilpa Chugh; Rao, Paturi Vishnupriya

    2015-05-01

    In patients with diabetes, treatment intensification requires basal and bolus insulin injections to control the fasting and prandial insulin needs. To overcome the burden of multiple daily injections, co-formulating basal and bolus insulins in single injection could allow a simple regimen with fewer injections. Current premixed insulin analogues are limited by the protaminated insulin component, which cannot provide effective basal coverage. While, long-acting insulin analogues like insulin glargine and insulin detemir cannot be combined with rapid-acting insulin analogues due to physicochemical incompatibility. Insulin degludec/insulin aspart (IDegAsp) is a soluble co-formulation of two distinct insulin analogues in the ratio of 70% ultra-long-acting insulin degludec (IDeg) and 30% rapid-acting insulin aspart (IAsp). The distinct PK/PD properties of IDeg and IAsp components are preserved in the co-formulation, with the rapid absorption characteristics of IAsp and flat and stable profile of IDeg maintained separately. Size exclusion chromatography studies of IDegAsp indicate that IDeg and lAsp exist as stable di-hexamers and hexamers, respectively in the formulation. Moreover, at steady state, the prandial and basal glucose lowering effects of IDeg and IAsp were distinct and clearly separated. A clear dose-response relationship was observed in patients with type 1 and type 2 diabetes treated with IDegAsp. The glucose lowering effects of basal and prandial components of IDegAsp are maintained in elderly (≥ 65 years of age) patients with type 1 diabetes. In addition, the PK and clearance of IDeg and IAsp are not affected by mild, moderate or severe renal or hepatic impairment. Presence of two distinct insulin analogues, as a soluble co-formulation with basal component with an ultra-long duration of action makes IDegAsp an advance to premix insulins.

  7. Geomorphic and hydrological effects of traditional shifting agriculture in a Mediterranean mountain, Central Spanish Pyrenees

    NARCIS (Netherlands)

    Lasanta, T.; Beguería, S.; García-Ruiz, J.M.

    2006-01-01

    Shifting agriculture occupied large areas of middle latitude mountains during periods of strong demographic pressure. On average, this practice accounted for about 22.8% of the total cultivated area in the Central Spanish Pyrenees at the beginning of the 20th century. The use of experimental

  8. Temporal and spatial interactions of slope and catchment processes in the Central Spanish Pyrenees

    NARCIS (Netherlands)

    Beguería, S.; García-Ruiz, J.M.; Lana-Renault, N.; Valero-Garcés, B.; Lasanta, T.; Arnáez, J.; López-Moreno, J.I.; Regüés, D.; Martí-Bono, C.

    2004-01-01

    Historical and present day changes in land uses and plant cover explain the complex interactions assessed in the Central Spanish Pyrenees between geomorphic processes in hillslopes and channels. More intense erosion periods caused an enlargement of sediment sources areas and an increase of

  9. Geological analysis of paleozoic large-scale faulting in the south-central Pyrenees

    NARCIS (Netherlands)

    Speksnijder, A.

    1986-01-01

    Detailed structural and sedimentological analysis reveals the existence of an east-west directed fundamental fault zone in the south-central Pyrenees, which has been intermittently active from (at least) the Devonian on. Emphasis is laid on the stUdy of fault-bounded post-Variscan (StephanoPermian)

  10. Geological analysis of paleozoic large-scale faulting in the south-central Pyrenees

    NARCIS (Netherlands)

    Speksnijder, A.

    1986-01-01

    Detailed structural and sedimentological analysis reveals the existence of an east-west directed fundamental fault zone in the south-central Pyrenees, which has been intermittently active from (at least) the Devonian on. Emphasis is laid on the stUdy of fault-bounded post-Variscan

  11. Contingency & Agency in the Holocene Anthropization of Mountain Landscapes of the Western Pyrenees

    Science.gov (United States)

    Gragson, Theodore; Coughlan, Michael; Leigh, David

    2017-04-01

    Mounting evidence indicates that mixed forests of the humid-temperate western Pyrenees mountains were converted by human agency to managed grasslands by at least the late Neolithic. We first realized major ramifications of the conversation process from pronounced differences we observed between soil profiles of ancient pastures and old-growth forests in otherwise similar landscape positions. Subsequently through radiocarbon dating of colluvial deposits we established a chronology for anthropic manipulation of the biotic factor of pedogenesis resulting in the creation of new soil materials, processes and functions. Regional- and biome-scale paleoecological analyses and archaeological syntheses suggest that it was Neolithic agropastoral land use that initiated anthropization of mountain landscapes of the western Pyrenees. However, such macroscopic views of human behavior cannot reveal the contingency and agency on which human causality rests. We have thus followed a complementary place-based investigative strategy that couples geoarchaeological, biophysical and socio-ecological factors spatially and temporally to arrive at the coevolutionary processes of human-environment interactions and landscape history. The results often contrast sharply with conventional narratives about human landscape degradation in agropastoral systems. For the last 2000 years, the western Pyrenees mountains were spatially removed from regional centers such as Pamplona and Bordeaux, and economically and politically peripheral to continental social and governmental processes. This marginality favored a macroscopic and time-invariant interpretation of agropastoral production in the western Pyrenees as the result of unambiguously enforced social norms exacted by intense solidarities of kin and neighbors. However, anthropization of the western Pyrenees was a spatially and temporally heterogeneous process in which land transitions appear to precede intensification. Radiocarbon dating shows three

  12. Lichens biomonitoring as feasible methodology to assess air pollution in natural ecosystems: combined study of quantitative PAHs analyses and lichen biodiversity in the Pyrenees Mountains.

    Science.gov (United States)

    Blasco, María; Domeño, Celia; Nerín, Cristina

    2008-06-01

    The air quality in the Aragón valley, in the central Pyrenees, has been assessed by evaluation of lichen biodiversity and mapped by elaboration of the Index of Air Purity (IAP) based on observations of the presence and abundance of eight kinds of lichen with different sensitivity to air pollution. The IAP values obtained have been compared with quantitative analytical measures of 16 PAHs in the lichen Evernia prunastri, because this species was associated with a wide range of traffic exposure and levels of urbanization. Analyses of PAHs were carried out by the DSASE method followed by an SPE clean-up step and GC-MS analysis. The concentration of total PAHs found in lichen samples from the Aragón valley ranged from 692 to 6420 ng g(-1) and the PAHs profile showed predominance of compounds with three aromatic rings. The influence of the road traffic in the area has been shown because values over the median concentration of PAHs (>1092 ng g(-1)), percentage of combustion PAHs (>50%), and equivalent toxicity (>169) were found in lichens collected at places exposed to the influence of traffic. The combination of both methods suggests IAP as a general method for evaluating the air pollution referenced to PAHs because it can be correlated with the content of combustion PAHs and poor lichen biodiversity can be partly explained by the air pollution caused by specific PAHs.

  13. Lichens biomonitoring as feasible methodology to assess air pollution in natural ecosystems: Combined study of quantitative PAHs analyses and lichen biodiversity in the Pyrenees Mountains

    Energy Technology Data Exchange (ETDEWEB)

    Blasco, Maria; Domeno, Celia; Nerin, Cristina [Grupo GUIA-Instituto de Investigacion de Ingenieria en Aragon (I3A), Centro Superior de Ingenieros (CPS), Zaragoza (Spain)

    2008-06-15

    The air quality in the Aragon valley, in the central Pyrenees, has been assessed by evaluation of lichen biodiversity and mapped by elaboration of the Index of Air Purity (IAP) based on observations of the presence and abundance of eight kinds of lichen with different sensitivity to air pollution. The IAP values obtained have been compared with quantitative analytical measures of 16 PAHs in the lichen Evernia prunastri, because this species was associated with a wide range of traffic exposure and levels of urbanization. Analyses of PAHs were carried out by the DSASE method followed by an SPE clean-up step and GC-MS analysis. The concentration of total PAHs found in lichen samples from the Aragon valley ranged from 692 to 6420 ng g{sup -1} and the PAHs profile showed predominance of compounds with three aromatic rings. The influence of the road traffic in the area has been shown because values over the median concentration of PAHs (>1092 ng g{sup -1}), percentage of combustion PAHs (>50%), and equivalent toxicity (>169) were found in lichens collected at places exposed to the influence of traffic. The combination of both methods suggests IAP as a general method for evaluating the air pollution referenced to PAHs because it can be correlated with the content of combustion PAHs and poor lichen biodiversity can be partly explained by the air pollution caused by specific PAHs. (orig.)

  14. Prerequisites for Affective Signal Processing (ASP) - Part II

    NARCIS (Netherlands)

    van den Broek, Egon; Janssen, Joris H.; Healey, Jennifer A.; van der Zwaag, Marjolein; Fred, A.; Filipe, J.; Gamboa, H.

    2010-01-01

    Last year, in van den Broek et al. (2009a), a start was made with defining prerequisites for affective signal processing (ASP). Four prerequisites were identified: validation (e.g., mapping of constructs on signals), triangulation, a physiology-driven approach, and contributions of the signal

  15. Prerequisites for Affective Signal Processing (ASP) - Part III

    NARCIS (Netherlands)

    van den Broek, Egon; Janssen, Joris H.; van der Zwaag, Marjolein D.; Healey, Jennifer A.; Fred, A.; Filipe, J.; Gamboa, H.

    2010-01-01

    This is the third part in a series on prerequisites for affective signal processing (ASP). So far, six prerequisites were identified: validation (e.g., mapping of constructs on signals), triangulation, a physiology-driven approach, and contributions of the signal processing community (van den Broek

  16. FROM IPAB TO ASP: THE EVOLUTION OF DECISIONAL PROCESSES

    Directory of Open Access Journals (Sweden)

    Dario Recubini

    2014-12-01

    Full Text Available This study aims to analyze the evolution of decision-making processes through the transformation of The Public Institution of Assistance and Charity (IPAB, in Public Corporations aimed to the Person (ASP. Public corporations of services oriented to the Person (ASP are, in facts, an innovation. In the Public sector, ASPs are of big relevance, providing health care services to the society and taking a new and innovative governance model. This model wants to increase he awareness of ASP’s role in the public sector and generate awareness of the social effects, coupled with cost effectiveness and competitiveness. The public-law Corporations, which have got legal subject, administrative, property, accounting and financial autonomy, perform their activity with efficiency, efficacy and cost effectiveness, keeping the right balance between costs and profits. They are also able to save, anyway, not less than a third of incomings to realize various activities. These kind of companies are active at council and provincial level. This process is managed by the Region which endorses its Statutes, promotes the establishment of tools for the valuation of property assets and regulates the information and accounting system.   DALLE IPAB ALLE ASP: L’EVOLUZIONE DEI PROCESSI DECISIONALI Lo studio intende analizzare l’evoluzione dei processi decisionali nella trasformazione delle Istituzioni Pubbliche di Assistenza e Beneficienza (IPAB in Aziende Servizi alla Persona (ASP. Le Aziende pubbliche di servizi alla persona (ASP rappresentano, infatti,  un soggetto innovativo di grande rilevanza nell'ambito dei soggetti pubblici produttori ed erogatori di servizi socio-sanitari assumendo un nuovo modello di governance innovativo, diretto ad aumentare la consapevolezza del ruolo svolto e degli effetti prodotti in termini sociali, economici e competitivi. Aziende di diritto pubblico, dotate di personalità giuridica, di autonomia statutaria, gestionale, patrimoniale

  17. HT-LP thermometamorphism modelling : Agly massif, French Pyrenees.

    Science.gov (United States)

    Tournaire Guille, Baptiste; Pascal, Marie-Lola; Lejeune, Anne-Marie; Annen, Catherine

    2017-04-01

    Owing to the strongly anomalous thermal gradients implied, HT-LP metamorphism is a worldwide type of processes in which magma emplacement and solidification at relatively high levels in the crust must be considered as a potentially major heat source. Thermal modelling (e.g. Annen et al. 2005) is an appropriate tool for constraining the part played by such processes in practical cases of thermometamorphism. We study the Agly massif, an exhumed part of middle crust from the Variscan belt in the French Pyrenees. This massif is a classical example of HT-LP metamorphism (Vielzeuf 1996), composed of a metasedimentary cover, mainly micaschists aged from upper Cambrian to Devonian, unconformably overlying an older basement of para- and orthogneisses. The Variscan metamorphic facies extend from greenschists, in the upper part of the cover, to granulites in the basement (Fonteilles 1976). The apparent geotherm of about 110°/km in the metasedimentary cover (amphibolite and greenschist facies) has given way to contrasting interpretations. Magmatic activity partly synchronous with and probably related to the Variscan thermometamorphism is observed at the outcropping level as at least 4 magmatic bodies of mantle origin (Touil 1994), of Stephanian age, including granodiorites and subordinate diorites and gabbros. Recent U/Pb datations on zircons (Tournaire-Guille et al., in prep) also reveal the presence of lower Cambrian magmatism in the gneisses, therefore confirming their interpretation as a pre-Variscan basement. The location (depth), volume (thickness), temperature (composition) and timing of magma emplacement are the parameters controlling the thermal effect to be modelled with a Matlab® code (Annen et al. 2005). In order to constrain these parameters, we have updated the lithostratigraphy and the PT conditions of the Variscan metamorphism in the Agly area. Mineralogic and petrologic data exploited in thermobarometric analyses compared with thermodynamic PerpleX modelling

  18. ASP for Minimal Entailment in a Rational Extension of SROEL

    OpenAIRE

    Giordano, Laura; Dupré, Daniele Theseider

    2016-01-01

    In this paper we exploit Answer Set Programming (ASP) for reasoning in a rational extension SROEL-R-T of the low complexity description logic SROEL, which underlies the OWL EL ontology language. In the extended language, a typicality operator T is allowed to define concepts T(C) (typical C's) under a rational semantics. It has been proven that instance checking under rational entailment has a polynomial complexity. To strengthen rational entailment, in this paper we consider a minimal model s...

  19. Antecedents to the adoption of ASPS in healthcare.

    Science.gov (United States)

    Randeree, Ebrahim; Judd, Susan P; Kishore, Rajiv; Rao, H Raghav

    2003-01-01

    The objective of this exploratory study was to identify drivers of adoption for a new form of information technology outsourcing--the ASP model--in the healthcare industry. Primary data were collected in January 2002 from a nationwide survey of senior-level healthcare information technology executives. Cost management (supplier presence, asset specificity, production costs, transaction costs, resource availability) and relative advantage (reliability, customizability, strategic alignment, and magnitude of potential loss) were found to have the largest influences on adoption behavior.

  20. Structural Elucidation of the DFG-Asp in and DFG-Asp out States of TAM Kinases and Insight into the Selectivity of Their Inhibitors

    Directory of Open Access Journals (Sweden)

    Abdellah Messoussi

    2014-10-01

    Full Text Available Structural elucidation of the active (DFG-Asp in and inactive (DFG-Asp out states of the TAM family of receptor tyrosine kinases is required for future development of TAM inhibitors as drugs. Herein we report a computational study on each of the three TAM members Tyro-3, Axl and Mer. DFG-Asp in and DFG-Asp out homology models of each one were built based on the X-ray structure of c-Met kinase, an enzyme with a closely related sequence. Structural validation and in silico screening enabled identification of critical amino acids for ligand binding within the active site of each DFG-Asp in and DFG-Asp out model. The position and nature of amino acids that differ among Tyro-3, Axl and Mer, and the potential role of these residues in the design of selective TAM ligands, are discussed.

  1. Bed load size distribution and flow conditions in a high mountain catchment of Central Pyrenees

    OpenAIRE

    Martínez Castroviejo, Ricardo

    1990-01-01

    The bed load size distribution caused by different types of flow are compared in a high mountain catchment located in the upper Gallego river basin (Central Spanish’ Pyrenees). Three kinds of hydrologic events could be defined: those triggered by heavy autumn rainfalls, those originated by isolated summer rainstorms and those promoted by snowmelting. Each one is characterized by a peculiar bed load size distribution. Thus, it could be demonstrated that the coarser fractions, above 30 mm in di...

  2. Cave levels as proxies for measuring post-orogenic uplift: Evidence from cosmogenic dating of alluvium-filled caves in the French Pyrenees

    Science.gov (United States)

    Calvet, M.; Gunnell, Y.; Braucher, R.; Hez, G.; Bourlès, D.; Guillou, V.; Delmas, M.; Aster Team

    2015-10-01

    The rates and chronology of valley incision in mountain ranges have been studied in various parts of the globe, but the causes of river incision are often blurred because tectonic, climatic, and sea level-related forcing signals are difficult to distinguish from one another. The Têt River limestone gorge in the Eastern Pyrenees, which displays multiple cave levels containing datable alluvial deposits, provides an opportunity for clarifying this debate. Horizontal epiphreatic passages in limestone can be used as substitutes for fluvial terraces because they correspond to former valley floors and, therefore, also record the position of former local base levels. In the Têt canyon, the passages are filled with quartz-rich sand and gravel sequences that can be dated by 26Al/10Be burial dating. The canyon has cut into a Middle Miocene pediment system-now forming a raised plateau at 1250-1500 m-and displays nine cave levels over a vertical height of 1 km. One alluvial fill sequence in a cave at + 270 m above datum (i.e., the local river bed) yielded a weighted mean age of 5.14 ± 0.41 Ma; another, situated at + 110 m above datum, yielded weighted mean ages of 2.23 ± 0.230 Ma and 1.20 ± 0.286 Ma. The data convert to a mean incision rate of 52 m·Ma- 1 since the beginning of the Pliocene, and involved an acceleration to 92 m·Ma- 1 during the Quaternary. Pre-burial catchment denudation rates range from 35 to 7 m·Ma- 1, and these also doubled during the early Quaternary. It is concluded that: (i) valley incision into the Miocene pediment has been occurring since 5, probably 10 Ma; (ii) there is no evidence of a Messinian canyon in the Villefranche gorge, strongly suggesting through various additional indicators that interference of the Messinian Salinity Crisis with the canyon incision history was minimal; (iii) valley deepening was not a steady process, and recorded periods of stability around 1-2 Ma and perhaps 6-5 Ma; and (iv) the terraced network of epiphreatic cave

  3. Description and analysis of major debris flows occurred during 2008 in the Eastern Pyrenees

    Science.gov (United States)

    Chevalier, G.; Postilla, M.; Hürlimann, M.

    2009-04-01

    In the Eastern Pyrenees, debris flows are not as frequent as in other mountainous areas such as the Alps. Nevertheless, several important events have occurred in 2008 provoking damages to essential infrastructures and causing large economic loss. A rainstorm at the beginning of June 2008 generated various surficial slides and debris flows in the area of Berga, located in the Pre-Pyrenees. A major flow obstructed the tunnel entrance of a national road during several days. Another rainstorm on August 1 caused several debris flows and debris floods in the Southern sector of Andorra, situated in the Axial Pyrenees. The most important event occurred in the Riu Runer torrent and destroyed the main building at the Andorran border. Finally, a large debris flow was triggered by a thunderstorm on September 11 near Rialp, Axial Pyrenees. Some installations of Port-Ainé's ski-resort were damaged and its access road was destroyed at several points. Preliminary results of these three events are presented focussing on the initiation, flow behaviour and deposition processes. Moreover, the influence of human activity on the initiation was analysed. Field surveys and interpretation of aerial photographs are carried out in order to obtain geomorphologic information as well as data on the hydraulic characteristics. Additionally, rainfall as triggering is studied using records from nearby observation stations and data from weather radars. Finally, the dynamic behaviour is simulated at one site applying numerical modelling. Volume estimates of the two events occurred in the Axial Pyrenees range from 5000 up to 10000 m3. These are rather large magnitudes compared to historic debris flows in the same area. The initiation process of both events can be defined as in-channel formation. Field observations indicated important erosion rates of up to 10 m3/m characteristic of the high flow velocities estimated along the flow trajectory. In contrast, the Berga event was caused by a surficial

  4. Hands on with ASP.NET MVC covering MVC 6

    CERN Document Server

    Sahay, Rahul

    2014-01-01

    MVC (Model-View-Controller) is the popular Microsoft technology which enables you to build dynamic, data-driven, mobile websites, TDD site. Hands-On with ASP.NET MVC is not only written for those who are going to have affair with MVC for the 1st time, rather it is written in such a way that even experienced professional will love reading this book. This book covers all the tiny steps on using MVC at its best. With complete practical tutorials to illustrate the concepts, you will step by step build one End to End application which covers below mentioned techniques - Controllers, Views, Models,

  5. "A shepherd has to invent": Poetic analysis of social-ecological change in the cultural landscape of the central Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    Maria E. Fernández-Giménez

    2015-12-01

    Full Text Available Since the mid-20th century, the Pyrenean pastoral social-ecological system (SES has undergone socioeconomic and demographic transformations leading to changes in grazing practices and a decline in the livestock industry. Land abandonment has contributed to an ecological transition from herbaceous vegetation cover to shrublands and forests, leading to a loss of ecosystem services, including biodiversity and forage. I interviewed 27 stockmen (ganaderos in two valleys of the central Pyrenees to document their traditional ecological knowledge and observations of environmental, social, economic, and cultural changes in the valleys. I used poetic analysis, a qualitative data analysis approach, to illustrate and analyze one ganadero's experience of social-ecological change. First, I created seven poems based on an interview transcript with this ganadero. Second, I analyzed the poetry I created, to see what new insights and understanding about system dynamics and the lived experience of SES change emerged from analysis of the transcript re-presented as poetry. Third, I compared key themes that emerged from this analysis with findings across the other 26 interviews. Fourth, I read the poems and presented the associated analysis to multiple audiences, to gauge their impact and effectiveness in communicating research findings. Finally, I synthesized across the themes raised in the seven poems. Poetic analysis revealed emotional and cultural dimensions of change, especially the importance of occupational and place identity, in the experience of the ganadero. The transcript re-presented as poetry portrayed the ganadero as an agent in creating and maintaining a cultural landscape and as both an adaptor and resister to SES change. Poetic analysis also uncovered telling contradictions, adaptive capacities, and barriers to adaptation in this SES that went unappreciated with conventional qualitative analysis approaches. This exploratory study illustrates the

  6. Paradoxical Glucose-Sensitizing yet Proinflammatory Effects of Acute ASP Administration in Mice

    Directory of Open Access Journals (Sweden)

    Alexandre Fisette

    2013-01-01

    Full Text Available Acylation stimulating protein (ASP is an adipokine derived from the immune complement system, which stimulates fat storage and is typically increased in obesity, type 2 diabetes, and cardiovascular disease. Using a diet-induced obesity (DIO mouse model, the acute effects of ASP on energy metabolism and inflammatory processes in vivo were evaluated. We hypothesized that ASP would specifically exert proinflammatory effects. C57Bl/6 wild-type mice were put on a high-fat-high-sucrose diet for 12 weeks. Mice were then subjected to both glucose and insulin tolerance tests, each manipulation being preceded by recombinant ASP or vehicle (control bolus injection. ASP supplementation increased whole-body glucose excursion, and this was accomplished with reduced concomitant insulin levels. However, ASP did not directly alter insulin sensitivity. ASP supplementation induced a proinflammatory phenotype, with higher levels of cytokines including IL-6 and TNF-α in plasma and in adipose tissue, liver, and skeletal muscle mRNA. Additionally, ASP increased M1 macrophage content of these tissues. ASP exerted a direct concentration-dependent role in the migration and M1 activation of cultured macrophages. Altogether, the in vivo and in vitro experiments demonstrate that ASP plays a role in both energy metabolism and inflammation, with paradoxical whole-body glucose-sensitizing yet proinflammatory effects.

  7. Deep structure of Pyrenees range (SW Europe) imaged by joint inversion of gravity and teleseismic delay time

    Science.gov (United States)

    Dufréchou, G.; Tiberi, C.; Martin, R.; Bonvalot, S.; Chevrot, S.; Seoane, L.

    2018-04-01

    We present a new model of the lithosphere and asthenosphere structure down to 300 km depth beneath the Pyrenees from the joint inversion of recent gravity and teleseismic data. Unlike previous studies, crustal correction were not applied on teleseismic data in order (i) to preserve the consistency between gravity data, which are mainly sensitive to the density structure of the crust.lithosphere, and travel time data, and (ii) to avoid the introduction of biases resulting from crustal reductions. The density model down to 100 km depth is preferentially used here to discuss the lithospheric structure of the Pyrenees, whereas the asthenospheric structure from 100 km to 300 km depth is discussed from our velocity model. The absence of a high density anomaly in our model between 30-100 km depth (except the Labourd density anomaly) in the northern part of the Pyrenees seems to preclude eclogitization of the subducted Iberian crust at the scale of the entire Pyrenean range. Local eclogitization of the deep Pyrenean crust beneath the western part of the Axial Zone (West of Andorra) associated with the positive Central density anomaly is proposed. The Pyrenean lithosphere in density and velocity models appears segmented from East to West. No clear relation between the along-strike segmentation and mapped major faults is visible in our models. The Pyrenees' lithosphere segments are associated to different seismicity pattern in the Pyrenees suggesting a possible relation between the deep structure of the Pyrenees and its seismicity in the upper crust. The concentration of earthquakes localized just straight up the Central density anomaly can result of the subsidence and/or delamination of an eclogitized Pyrenean deep root. The velocity model in the asthenosphere is similar to previous studies. The absence of a high-velocity anomaly in the upper mantle and transition zone (i.e. 125 to 225 km depth) seems to preclude the presence of a detached oceanic lithosphere beneath the

  8. Long-term dynamics of Mycoplasma conjunctivae at the wildlife-livestock interface in the Pyrenees.

    Science.gov (United States)

    Fernández-Aguilar, Xavier; Cabezón, Oscar; Frey, Joachim; Velarde, Roser; Serrano, Emmanuel; Colom-Cadena, Andreu; Gelormini, Giuseppina; Marco, Ignasi; Mentaberre, Gregorio; Lavín, Santiago; López-Olvera, Jorge Ramón

    2017-01-01

    Functional roles of domestic and wild host populations in infectious keratoconjunctivitis (IKC) epidemiology have been extensively discussed claiming a domestic reservoir for the more susceptible wild hosts, however, based on limited data. With the aim to better assess IKC epidemiology in complex host-pathogen alpine systems, the long-term infectious dynamics and molecular epidemiology of Mycoplasma conjunctivae was investigated in all host populations from six study areas in the Pyrenees and one in the Cantabrian Mountains (Northern Spain). Detection of M. conjunctivae was performed by qPCR on 3600 eye swabs collected during seven years from hunted wild ungulates and sympatric domestic sheep (n = 1800 animals), and cluster analyses of the strains were performed including previous reported local strains. Mycoplasma conjunctivae was consistently detected in three Pyrenean chamois (Rupicapra p. pyrenaica) populations, as well as in sheep flocks (17.0% of sheep) and occasionally in mouflon (Ovis aries musimon) from the Pyrenees (22.2% in one year/area); statistically associated with ocular clinical signs only in chamois. Chamois populations showed different infection dynamics with low but steady prevalence (4.9%) and significant yearly fluctuations (0.0%- 40.0%). Persistence of specific M. conjunctivae strain clusters in wild host populations is demonstrated for six and nine years. Cross-species transmission between chamois and sheep and chamois and mouflon were also sporadically evidenced. Overall, independent M. conjunctivae sylvatic and domestic cycles occurred at the wildlife-livestock interface in the alpine ecosystems from the Pyrenees with sheep and chamois as the key host species for each cycle, and mouflon as a spill-over host. Host population characteristics and M. conjunctivae strains resulted in different epidemiological scenarios in chamois, ranging from the fading out of the mycoplasma to the epidemic and endemic long-term persistence. These findings

  9. Long-term dynamics of Mycoplasma conjunctivae at the wildlife-livestock interface in the Pyrenees.

    Directory of Open Access Journals (Sweden)

    Xavier Fernández-Aguilar

    Full Text Available Functional roles of domestic and wild host populations in infectious keratoconjunctivitis (IKC epidemiology have been extensively discussed claiming a domestic reservoir for the more susceptible wild hosts, however, based on limited data. With the aim to better assess IKC epidemiology in complex host-pathogen alpine systems, the long-term infectious dynamics and molecular epidemiology of Mycoplasma conjunctivae was investigated in all host populations from six study areas in the Pyrenees and one in the Cantabrian Mountains (Northern Spain. Detection of M. conjunctivae was performed by qPCR on 3600 eye swabs collected during seven years from hunted wild ungulates and sympatric domestic sheep (n = 1800 animals, and cluster analyses of the strains were performed including previous reported local strains. Mycoplasma conjunctivae was consistently detected in three Pyrenean chamois (Rupicapra p. pyrenaica populations, as well as in sheep flocks (17.0% of sheep and occasionally in mouflon (Ovis aries musimon from the Pyrenees (22.2% in one year/area; statistically associated with ocular clinical signs only in chamois. Chamois populations showed different infection dynamics with low but steady prevalence (4.9% and significant yearly fluctuations (0.0%- 40.0%. Persistence of specific M. conjunctivae strain clusters in wild host populations is demonstrated for six and nine years. Cross-species transmission between chamois and sheep and chamois and mouflon were also sporadically evidenced. Overall, independent M. conjunctivae sylvatic and domestic cycles occurred at the wildlife-livestock interface in the alpine ecosystems from the Pyrenees with sheep and chamois as the key host species for each cycle, and mouflon as a spill-over host. Host population characteristics and M. conjunctivae strains resulted in different epidemiological scenarios in chamois, ranging from the fading out of the mycoplasma to the epidemic and endemic long-term persistence. These

  10. 700 years reconstruction of mercury and lead atmospheric deposition in the Pyrenees (NE Spain)

    Science.gov (United States)

    Corella, J. P.; Valero-Garcés, B. L.; Wang, F.; Martínez-Cortizas, A.; Cuevas, C. A.; Saiz-Lopez, A.

    2017-04-01

    Geochemical analyses in varved lake sediment cores (Lake Montcortès, Pre-Pyrenees) allowed reconstruction of mercury (Hg) and lead (Pb) atmospheric deposition over the past seven centuries in the Pyrenees (NE Spain). Accumulation Rates (AR) from the Middle Ages to the Industrial Period ranged from 2500 to 26130 μg m2.y-1 and 15-152 μg m2.y-1 for Pb and Hg respectively. Significant metal pollution started ca CE 1550 during a period of increased exploitation of ore resources in Spain. Colder and humid conditions in the Pyrenees during the Little Ice Age may have also favoured Hg and Pb atmospheric deposition in the lake. Therefore, the interplay between increased rainfall (wet deposition) and mining activities in the Iberian Peninsula has driven Hg and Pb AR during the Pre-industrial Period. More recently, the use of leaded gasoline in Europe in the mid-20th century may explain the highest Pb AR between CE 1953 and 1971. The highest Hg AR occurred in CE 1940 synchronous with the highest Hg production peak in Almadén mining district (southern Spain) and the Second World War. The record of Hg enrichment in Lake Montcortès shows a decrease during the last decades in Western Europe similar to other regional records and global emission models. This study highlights the exceptional quality of varved sequences to tease apart pollutants depositional mechanisms, identify historical periods of increased atmospheric pollution and provide a historical context for pollutant baseline values to make correct assessments of recent (atmospheric) pollution in lake ecosystems.

  11. A Structural Equation Modelling for CRM Development in rural Tourism in the Catalan Pyrenees

    Directory of Open Access Journals (Sweden)

    José Mª Prat Forga

    2014-12-01

    Full Text Available This paper investigates the interrelationships between customer relationship management development in rural tourism, information and communication technologies level in the territory, perceived economic impacts and rural tourism development. A total of 76 respondents completed a survey conducted in the Spanish Pyrenees Mountains in order to examine the structural effects of these impact factors. The results reveal that the support for customer relationship management development in rural tourism shown by rural tourism workers mainly depends on the level of development of information and communication technologies. A confirmatory factor analysis and structural equation modelling procedure were performed, respectively, using the AMOS software. 

  12. A Structural Equation Modelling for Crm Development in Rural Tourism in the Catalan Pyrenees

    Directory of Open Access Journals (Sweden)

    José Mª Prat Forga

    2013-02-01

    Full Text Available This paper investigates the interrelationships between customer relationship management development in rural tourism, information and communication technologies level in the territory, perceived economic impacts and rural tourism development. A total of 76 respondents completed a survey conducted in the Spanish Pyrenees Mountains in order to examine the structural effects of these impact factors. The results reveal that the support for customer relationship management development in rural tourism shown by rural tourism workers mainly depends on the level of development of information and communication technologies. A confirmatory factor analysis and structural equation modelling procedure were performed, respectively, using the AMOS software.

  13. Stratigraphic and structural reconstruction of an Upper Ordovician super-eruption (Catalan Pyrenees)

    Science.gov (United States)

    Marti, Joan; Casas, Josep Maria; Muñoz, Josep A.

    2017-04-01

    Pre-Variscan basement of the Pyrenees includes evidence of many magmatic episodes represented by different types of granitoids and volcanic rocks, which indicates the complex geodynamic history of this peri-Gondwana terrane during Palaeozoic. One of the most significative magmatic episodes is that of Upper Ordovician (Caradocian) age, which is represented by several granitic and granodioritic bodies and volcanic rocks mostly of pyroclastic nature. In the Catalan Pyrenees this magmatism is well represented in the Ribes de Freser and Nuria area, where the orthogneisses from the Nuria massif and the Ribes granophyre, both with a similar age of 457 Ma, seem to form a calc-alkaline plutonic suite covering terms from deeper to shallower levels. The presence of numerous pyroclastic deposits and lavas interbedded with Caradocian sediments and intruded by and immediately above the Ribes granophyre, suggests that this intrusive episode also generated significant volcanism. The area also hosts an important volume of rhyolitic ignimbrites and andesitic lavas strongly affected by Alpine tectonics and commonly showing tectonised contacts at the base and top of the sequences. These volcanic rocks were previously attributed to the Upper Carboniferous late-Variscan volcanism, extensively represented in the Pyrenees. However, new laser ablation U-Pb zircon geochronology from these rocks has revealed an Upper Ordovician age ( 455 Ma), similar to that of the plutonic rocks of the same area, thus suggesting a probable genetic relation between all them. The palinspatic reconstruction of the Alpine and Variscan tectonic units that affect this area has permitted to infer the geometry, facies distribution, original position, and thickness of these volcanic rocks previously attributed to the late-Variscan volcanism, and reveals how they are spatially (and stratigraphically) associated with the previously identified Late Ordovician volcanic rocks. In particular, the volcanic rocks cropping

  14. Professional ASP.NET 4.5 in C# and VB

    CERN Document Server

    Gaylord, Jason N; Rastogi, Pranav; Miranda, Todd; Hanselman, Scott

    2013-01-01

    The all-new approach for experienced ASP.NET professionals! ASP.NET is Microsoft's technology for building dynamically generated web pages from database content. Originally introduced in 2002, ASP.NET has undergone many changes in multiple versions and iterations as developers have gained a decade of experience with this popular technology. With that decade of experience, this edition of the book presents a fresh, new overhauled approach. A new focus on how to build ASP.NET sites and applications relying on field-tested reliable methodsIntegration of ""One A

  15. An All-Sky Portable (ASP) Optical Catalogue

    Science.gov (United States)

    Flesch, Eric Wim

    2017-06-01

    This optical catalogue combines the all-sky USNO-B1.0/A1.0 and most-sky APM catalogues, plus overlays of SDSS optical data, into a single all-sky map presented in a sparse binary format that is easily downloaded at 9 Gb zipped. Total count is 1 163 237 190 sources and each has J2000 astrometry, red and blue magnitudes with PSFs and variability indicator, and flags for proper motion, epoch, and source survey and catalogue for each of the photometry and astrometry. The catalogue is available on http://quasars.org/asp.html, and additional data for this paper is available at http://dx.doi.org/10.4225/50/5807fbc12595f.

  16. The fourth edition of the ASP took place in Kigali

    CERN Document Server

    CERN Bulletin

    2016-01-01

    The fourth biennial African School of Fundamental Physics and Applications (ASP) took place on 1-19 August in Africa’s cleanest city, Kigali in Rwanda.   The students of the fourth African School of Fundamental Physics and Applications pose for the traditional conference group picture. Many lecturers flew in from CERN to give lectures and mentor students. (Photo: Gilbert Tekoute) Seventy-five students from around the African continent, chosen from 439 applicants, were hosted in the University of Rwanda’s College of Sciences and Technology for about 3 weeks. The school received financial support from CERN and 19 other institutions in total, including the International Centre for Theoretical Physics (ICTP), Brookhaven National Laboratory, the South African National Research Foundation and Department of Technology, the Rwandan Ministry of Education, INFN, and other major particle physics laboratories, as well as governmental institutions in Africa, Europe and the United States....

  17. Subalpine Pyrenees received higher nitrogen deposition than predicted by EMEP and CHIMERE chemistry-transport models

    Science.gov (United States)

    Boutin, Marion; Lamaze, Thierry; Couvidat, Florian; Pornon, André

    2015-08-01

    Deposition of reactive nitrogen (N) from the atmosphere is expected to be the third greatest driver of biodiversity loss by the year 2100. Chemistry-transport models are essential tools to estimate spatially explicit N deposition but the reliability of their predictions remained to be validated in mountains. We measured N deposition and air concentration over the subalpine Pyrenees. N deposition was found to range from 797 to 1,463 mg N m-2 year-1. These values were higher than expected from model predictions, especially for nitrate, which exceeded the estimations of EMEP by a factor of 2.6 and CHIMERE by 3.6. Our observations also displayed a reversed reduced-to-oxidized ratio in N deposition compared with model predictions. The results highlight that the subalpine Pyrenees are exposed to higher levels of N deposition than expected according to standard predictions and that these levels exceed currently recognized critical loads for most high-elevation habitats. Our study reveals a need to improve the evaluation of N deposition in mountains which are home to a substantial and original part of the world’s biodiversity.

  18. The sedimentary record and petrophysical logs from the Spanish Central Pyrenees: Implications for paleoclimate change in the Early Devonian

    Czech Academy of Sciences Publication Activity Database

    Slavík, Ladislav; Valenzuela-Ríos, J. I.; Hladil, Jindřich; Chadimová, Leona; Liao, J-Ch.; Hušková, Aneta; Calvo, H.; Hrstka, Tomáš

    2015-01-01

    Roč. 21, - (2015), s. 350-350 ISSN 1608-8166. [International Congress on Stratigraphy /2./. STRATI 2015. 19.07.2015-23.07.2015, Graz] Institutional support: RVO:67985831 Keywords : stratigraphy * Early Devonian * sedimentary record * paleoclimate change * Spanish Central Pyrenees Subject RIV: DB - Geology ; Mineralogy http://www.zobodat.at/pdf/Ber-Inst-Erdwiss-Univ-Graz_21_0001-0437.pdf

  19. Chronology and pedogenic effects of mid- to late-Holocene conversion of forests to pastures in the French western Pyrenees

    Science.gov (United States)

    David S. Leigh; Theodore L. Gragson; Michael R. Coughlan

    2015-01-01

    This paper presents a place-based examination of the timing and long-term pedogenic effects of human-induced forest to pasture conversion in the French western Pyrenees Mountains, Basque commune of Larrau. We analyzed colluvial stratigraphic sections to derive the chronology of landscape change using radiocarbon dating, charcoal concentrations, magnetic...

  20. Absence of GABRA1 Ala322Asp mutation in juvenile myoclonic ...

    Indian Academy of Sciences (India)

    An Ala322Asp mutation in the GABRA1 gene was recently reported to be responsible for causing the autosomal dominant (AD) form of juvenile myoclonic epilepsy (JME) in a French-Canadian family. To study if JME families from India exhibiting the AD mode of inheritance carry the Ala322Asp mutation, we examined 35 ...

  1. Isomerization of Asp residues plays an important role in αA-crystallin dissociation.

    Science.gov (United States)

    Takata, Takumi; Fujii, Noriko

    2016-03-01

    Aged cataract formation is caused by the accumulative precipitation of lens proteins incorporating diverse post-translational modifications. α-Crystallin, a major structural and functional lens protein, consists of a large polymeric structure that is dissociated and insolubilized with accumulative post-translational modifications. One such modification, isomerization of Asp, was recently identified in αB-crystallin monomers derived from aged lens. However, the distributions of Asp isomers in each lens fraction remain unknown. Here, α-crystallin fractions from aged lens were separated into heteropolymeric and monomeric forms to determine the Asp isomerization ratios in each fraction. Lens of four different ages were homogenized and centrifuged, and the soluble fraction was applied to size-exclusion chromatography. The heteropolymeric α-crystallin and monomeric crystallin fractions were obtained and concentrated. After trypsin digestion, each fraction was independently applied to liquid chromatography equipped with mass spectrometry to extract α-crystallin-derived peptides containing Asp isomers. The results showed that Asp58, Asp84 and Asp151 of αA-crystallin were highly isomerized in the monomeric fraction, but not isomerized to the same level in the heteropolymeric fraction. Each type of Asp isomerization increased in an age-dependent manner, was site-specific and was similar to previous results from lens water-insoluble fractions. These results imply that isomerization of Asp residues leads to dissociation of αA-crystallin from the heteropolymeric state and induces insolubilization in aged lens. Taken together, our findings suggest that isomerization of Asp might disrupt the higher order polymeric state of α-crystallin, resulting in decreased solubility and function, ultimately contributing to lens protein impairment and cataract formation with aging. © 2015 Federation of European Biochemical Societies.

  2. Structural evolution of Pico del Águila anticline (External Sierras, southern Pyrenees) derived from sandbox, numerical and 3D structural modelling techniques

    OpenAIRE

    VIDAL-ROYO, O.; MUÑOZ, J.A.; HARDY, S.; KOYI, H.; CARDOZO, N.

    2013-01-01

    This paper reports on the integration of different modelling techniques to construct a unified conceptual model of structural evolution of the Pico del Águila anticline (External Sierras, southern Pyrenees, Spain). The structure is a well-known example of a detachment fold, which exhibits a N-S structural trend, perpendicular to the general structural trend of the southern Pyrenees (mainly E-W). Based on field observations of an unevenly distributed Triassic décollement, analogue modelling sh...

  3. Association of Glu298Asp Polymorphism of Endothelial NO Synthase Gene with Metabolic Syndrome Development: a Pilot Study.

    Science.gov (United States)

    Fattakhov, N S; Skuratovskaya, D A; Vasilenko, M A; Kirienkova, E V; Zatolokin, P A; Mironyuk, N I; Litvinova, L S

    2017-03-01

    We studied association of single nucleotide polymorphism Glu298Asp (rs1799983) of the NOS3 gene with the risk of metabolic syndrome in the Slavic population. Blood samples were obtained from 128 patients with metabolic syndrome and 100 healthy individuals. Polymorphism Glu298Asp of the NOS3 gene was genotyped by allele-specific PCR. Allele Asp (OR=1.95, 95%CI 1.29-2.95, p=0.007) and genotype Asp/Asp (OR=2.56, 95%CI 0.98-6.72, p=0.04) were associated with the risk of metabolic syndrome in Slavic population. Patients with metabolic syndrome carrying genotype Asp/Asp had higher serum endothelin-1 level in comparison with Glu/Asp and Glu/Glu carriers.

  4. Growth and destruction of Bathonian silica nodules in the Western Pyrenees (France)

    Science.gov (United States)

    James, V.; Canerot, J.; Meyer, A.; Biteau, J.-J.

    2000-04-01

    In the beginning of the Middle Jurassic, a regional ramp characterized by microfilament limestones was formed in the Western Pyrenean region. In the Middle Bathonian, it became a rimmed platform where silica nodules are formed. These nodules were studied in four sections along an East-West transverse area involving the newly developed inner and outer shelf carbonate environments. Petrographic and geochemical studies show that they are formed, in the whole domain, under shallow marine conditions, during the Middle-Upper Bathonian by the mixing of seawater and freshwater. Then, at the inner eastern platform, where carbonate strata were more permeable, they suffered an early dolomitization and, in the Albian, a subsequent desilicification and transformation into talc and calcite. The Bathonian nodule-bearing horizon was deposited near the top of a transgressive systems tract, close to the main flooding surface of a third-order sequence. Thus, it appears to provide a good stratigraphic marker all over the Western Pyrenees.

  5. Genetic diversity of planktonic eukaryotes in high mountain lakes (Central Pyrenees, Spain).

    Science.gov (United States)

    Triadó-Margarit, Xavier; Casamayor, Emilio O

    2012-09-01

    The genetic diversity of planktonic eukaryotic microorganisms (size range 3-40 µm) inhabiting 11 alpine lakes of the Central Pyrenees (Spain) was analysed by cloning and sequencing of the 18S rRNA gene. The selected lakes covered a wide range of environmental conditions representative of the regional landscape heterogeneity. Overall, we obtained 953 sequences (averaged length 750 bp) that were grouped in 343 representative OTUs (98% identity). The genetic richness was high, and the 18S rRNA gene sequences spread within nine high-rank taxonomic groups and grouped in 26 eukaryal classes. Most of the sequences affiliated with Stramenopiles (> 55% of total sequences, mostly Chrysophyceae), Cryptophyta and Alveolata (15% each). Three groups had relative abundance biodiversity. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

  6. Detecting human mobility in the Pyrenees through the analysis of chert tools during the Upper Palaeolithic

    Directory of Open Access Journals (Sweden)

    Marta Sánchez de la Torre

    2014-03-01

    Full Text Available The purpose of this paper is to present the preliminary results of PhD research focused on hunter-gatherer groups that occupied the Central and Eastern Pyrenees during the Magdalenian period. This research aims to improve the knowledge we have about those Magdalenian groups, specifically concerning their lithic procurement strategies. The core of the study is based on the lithic tools collected from two archaeological sites - Alonsé Cave and Forcas I Shelter, both in Huesca, Spain-, and in particular those made from chert, because they are both a spatial and a cultural marker at the same time. These cherts have been studied using petroarchaeological methods, and as a result, it has been possible to detect the type of procurement strategies carried out and to guess the relation existing between those human groups and their environment, especially in what refers to mobility strategies.

  7. Groundwater ferricretes from the Silurian of Ireland and Permian of the Spanish Pyrenees

    Science.gov (United States)

    Wright, V. P.; Sloan, R. J.; Valero Garcés, B.; Garvie, L. A. J.

    1992-04-01

    Haematite nodule horizons occur in alluvial-lacustrine deposits from the Mill Cove Formation (Silurian) of Dingle, Ireland and the Unidad Roja Superior (Permian) of the Spanish Pyrenees. These horizons, generally less than 1 m thick, are not associated with palaeosol profiles but are hosted by laminated or structureless, bioturbated mudstones to sandstones. The sequences contain weakly developed calcrete profiles. The haematite nodules are interpreted as having formed around fluctuating water-tables and are analogous to present-day groundwater ferricretes (laterites or plinthites). Fe 2+, mobilised during periods of reducing groundwaters, was segregated into ferric oxide nodules during falls in the water-table or during influxes of oxygenated meteoric waters. The prevailing climate during deposition of both sequences was probably semi-arid and the presence of ferricrete-like horizons is not evidence of humid climates. The horizons represent early "diagenetic" effects, not related to prevailing soil moisture regime.

  8. Arabidopsis SUMO protease ASP1 positively regulates flowering time partially through regulating FLC stability 

    KAUST Repository

    Kong, Xiangxiong

    2016-12-07

    The initiation of flowering is tightly regulated by the endogenous and environment signals, which is crucial for the reproductive success of flowering plants. It is well known that autonomous and vernalization pathways repress transcription of FLOWERING LOCUS C (FLC), a focal floral repressor, but how its protein stability is regulated remains largely unknown. Here, we found that mutations in a novel Arabidopsis SUMO protease 1 (ASP1) resulted in a strong late-flowering phenotype under long-days, but to a lesser extent under short-days. ASP1 localizes in the nucleus and exhibited a SUMO protease activity in vitro and in vivo. The conserved Cys-577 in ASP1 is critical for its enzymatic activity, as well as its physiological function in the regulation of flowering time. Genetic and gene expression analyses demonstrated that ASP1 promotes transcription of positive regulators of flowering, such as FT, SOC1 and FD, and may function in both CO-dependent photoperiod pathway and FLC-dependent pathways. Although the transcription level of FLC was not affected in the loss-of-function asp1 mutant, the protein stability of FLC was increased in the asp1 mutant. Taken together, this study identified a novel bona fide SUMO protease, ASP1, which positively regulates transition to flowering at least partly by repressing FLC protein stability.

  9. Meteorological and snow distribution data in the Izas Experimental Catchment (Spanish Pyrenees) from 2011 to 2017

    Science.gov (United States)

    Revuelto, Jesús; Azorin-Molina, Cesar; Alonso-González, Esteban; Sanmiguel-Vallelado, Alba; Navarro-Serrano, Francisco; Rico, Ibai; López-Moreno, Juan Ignacio

    2017-12-01

    This work describes the snow and meteorological data set available for the Izas Experimental Catchment in the Central Spanish Pyrenees, from the 2011 to 2017 snow seasons. The experimental site is located on the southern side of the Pyrenees between 2000 and 2300 m above sea level, covering an area of 55 ha. The site is a good example of a subalpine environment in which the evolution of snow accumulation and melt are of major importance in many mountain processes. The climatic data set consists of (i) continuous meteorological variables acquired from an automatic weather station (AWS), (ii) detailed information on snow depth distribution collected with a terrestrial laser scanner (TLS, lidar technology) for certain dates across the snow season (between three and six TLS surveys per snow season) and (iii) time-lapse images showing the evolution of the snow-covered area (SCA). The meteorological variables acquired at the AWS are precipitation, air temperature, incoming and reflected solar radiation, infrared surface temperature, relative humidity, wind speed and direction, atmospheric air pressure, surface temperature (snow or soil surface), and soil temperature; all were taken at 10 min intervals. Snow depth distribution was measured during 23 field campaigns using a TLS, and daily information on the SCA was also retrieved from time-lapse photography. The data set (https://doi.org/10.5281/zenodo.848277" target="_blank">https://doi.org/10.5281/zenodo.848277) is valuable since it provides high-spatial-resolution information on the snow depth and snow cover, which is particularly useful when combined with meteorological variables to simulate snow energy and mass balance. This information has already been analyzed in various scientific studies on snow pack dynamics and its interaction with the local climatology or topographical characteristics. However, the database generated has great potential for understanding other environmental processes from a hydrometeorological

  10. Spatial variations of b-value and crustal stress in the Pyrenees

    Science.gov (United States)

    Rigo, A.; Souriau, A.; Sylvander, M.

    2018-01-01

    The seismicity in the Pyrenees is continuous and well surveyed since more than 20 years. We use the catalogue of seismicity between 1997 and 2013 to explore the spatial variations of the b-value, which corresponds to the slope of the frequency-magnitude distribution of the earthquakes. Especially, variations of the b-value characterise the state of stress of the crust, possibly highlighting a deficit of large earthquake occurrence. We estimate the differential crustal stress from the b-value using a relationship published by Scholz ( Geophys Res Lett 42:1399-1402, 2015). We also estimate the stress drop variations by determining a power law which links the magnitude to the seismic source radius in the Pyrenees. We focus on the depth variations and we analyse vertical profiles of b-value, differential stress and stress drop, first in the whole Pyrenean belt, then in 10 subregions. The b-values are generally smaller than 1, except in the uppermost 3-5 km where the obtained high values could be linked to the presence of fluids. Downward, the b-values decrease slowly or remain constant until a depth of increase, which could correspond to the brittle-ductile limit of the crust. We propose that this depth and the regional and vertical variations of the b-values are related to the regional tectonic context and possibly to the density heterogeneities. We also suggest that stress drop and differential stress are linearly correlated and that the stress drop is at least 1.8‰ of the differential stress.

  11. Association between the ERCC5 Asp1104His polymorphism and cancer risk: a meta-analysis.

    Directory of Open Access Journals (Sweden)

    Mei-Ling Zhu

    Full Text Available BACKGROUND: Excision repair cross complementing group 5 (ERCC5 or XPG plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light. Mutations in this gene cause a rare autosomal recessive syndrome, and its functional single nucleotide polymorphisms (SNPs may alter DNA repair capacity phenotype and cancer risk. However, a series of epidemiological studies on the association between the ERCC5 Asp1104His polymorphism (rs17655, G>C and cancer susceptibility generated conflicting results. METHODOLOGY/PRINCIPAL FINDINGS: To derive a more precise estimation of the association between the ERCC5 Asp1104His polymorphism and overall cancer risk, we performed a meta-analysis of 44 published case-control studies, in which a total of 23,490 cases and 27,168 controls were included. To provide additional biological plausibility, we also assessed the genotype-gene expression correlation from the HapMap phase II release 23 data with 270 individuals from 4 ethnic populations. When all studies were pooled, we found no statistical evidence for a significantly increased cancer risk in the recessive genetic models (His/His vs. Asp/Asp: OR = 0.99, 95% CI: 0.92-1.06, P = 0.242 for heterogeneity or His/His vs. Asp/His + Asp/Asp: OR = 0.98, 95% CI: 0.93-1.03, P = 0.260 for heterogeneity, nor in further stratified analyses by cancer type, ethnicity, source of controls and sample size. In the genotype-phenotype correlation analysis from 270 individuals, we consistently found no significant correlation of the Asp1104His polymorphism with ERCC5 mRNA expression. CONCLUSIONS/SIGNIFICANCE: This meta-analysis suggests that it is unlikely that the ERCC5 Asp1104His polymorphism may contribute to individual susceptibility to cancer risk.

  12. Functional effects of the DCM mutant Gly159Asp troponin C in skinned muscle fibres

    DEFF Research Database (Denmark)

    Preston, Laura C; Lipscomb, Simon; Robinson, Paul

    2006-01-01

    We recently reported a dilated cardiomyopathy (DCM) causing mutation in a novel disease gene, TNNC1, which encodes cardiac troponin C (TnC). We have determined how this mutation, Gly159Asp, affects contractile regulation when incorporated into muscle fibres. Endogenous troponin in rabbit skinned...... psoas fibres was partially replaced by recombinant human cardiac troponin containing either wild-type or Gly159Asp TnC. We measured both the force-pCa relationship of these fibres and the activation rate using the caged-Ca(2+) compound nitrophenyl-EGTA. Gly159Asp TnC had no significant effect on either...

  13. Mechanism of Asp24 Upregulation in Brucella abortus Rough Mutant with a Disrupted O-Antigen Export System and Effect of Asp24 in Bacterial Intracellular Survival

    Science.gov (United States)

    Tian, Mingxing; Qu, Jing; Han, Xiangan; Ding, Chan; Wang, Shaohui; Peng, Daxin

    2014-01-01

    We previously showed that Brucella abortus rough mutant strain 2308 ΔATP (called the ΔrfbE mutant in this study) exhibits reduced intracellular survival in RAW264.7 cells and attenuated persistence in BALB/c mice. In this study, we performed microarray analysis to detect genes with differential expression between the ΔrfbE mutant and wild-type strain S2308. Interestingly, acid shock protein 24 gene (asp24) expression was significantly upregulated in the ΔrfbE mutant compared to S2308, as confirmed by quantitative reverse transcription-PCR (qRT-PCR) and Western blotting. Further studies using additional strains indicated that the upregulation of asp24 occurred only in rough mutants with disrupted O-antigen export system components, including the ATP-binding protein gene rfbE (bab1_0542) and the permease gene rfbD (bab1_0543), while the ΔwboA rough mutant (which lacks an O-antigen synthesis-related glycosyltransferase) and the RB51 strain (a vaccine strain with the rough phenotype) showed no significant changes in asp24 expression compared to S2308. In addition, abolishing the intracellular O-antigen synthesis of the ΔrfbE mutant by deleting the wboA gene (thereby creating the ΔrfbE ΔwboA double-knockout strain) recovered asp24 expression. These results indicated that asp24 upregulation is associated with intracellular O-antigen synthesis and accumulation but not with the bacterial rough phenotype. Further studies indicated that asp24 upregulation in the ΔrfbE mutant was associated neither with bacterial adherence and invasion nor with cellular necrosis on RAW264.7 macrophages. However, proper expression of the asp24 gene favors intracellular survival of Brucella in RAW264.7 cells and HeLa cells during an infection. This study reveals a novel mechanism for asp24 upregulation in B. abortus mutants. PMID:24752516

  14. Biodiversity and Greenhouse Gases in Grasslands: A Flux Network along Climate and Management Gradients in the Pyrenees

    Science.gov (United States)

    Sebastia, M. T.; Debouk, H.; Ibañez, M.; Llurba, R.; Ribas, A.; Altimir, N.

    2016-12-01

    Grassland in the Pyrenees is critically threatened by climate and land use changes. Ecologically, these ecosystems contain a rich biodiversity and contribute many ecosystem services, including feed production, soil carbon storage and water regulation. Socioeconomically, grasslands fuel the rural economy in the Pyrenees, including organic stockbreeding, outdoor activities and recreation. Three eddy covariance stations were established in the Eastern Pyrenees between 2009 and 2011 along altitudinal and climatic gradients by the FLUXPYR project (EU INTERREG IV-A-POCTEFA). The network of flux towers on climatically diverse grasslands is included in the European Fluxes Database Cluster. The three locations are representative of the typical managements in the region: Pla de Riart (ES-PRt, 1000 m a.s.l.), intensively managed sown grassland grazed in the fall; La Bertolina (ES-LBr, 1300 m a.s.l.), montane grassland grazed from spring to fall; and Castellar de n'Hug (ES-Cst, 2000 m a.s.l.), subalpine grassland grazed in summer. The flux studies are completed by chamber measurements of CO2, CH4, and N2O fluxes by photoacoustic. The focus of study is the relationship biodiversity-ecosystem function in response to climate, management and other environmental changes. Results show how sown and spontaneous plant diversity regulates grassland functioning including greenhouse gas fluxes. Polycultures in particular have a tendency to increase NEE and Reco compared to monocultures, enhancing CO2 uptake. Grasses are less efficient photosynthetically per biomass unit than other plant functional types, but this is not reflected at the ecosystem scale, where they are dominant. N2O is a greenhouse gas particularly dependent on plant functional diversity and climatic conditions. Soil function responds to changes in plant functional type distribution within the grassland. In conclusion, biodiversity regulates greenhouse gas fluxes in many ways in grasslands in the Pyrenees, through multiple

  15. Development of alkaline/surfactant/polymer (ASP flooding technology for recovery of Karazhanbas oil

    Directory of Open Access Journals (Sweden)

    Birzhan Zhappasbaev

    2016-03-01

    Full Text Available The tertiary oil recovery methods like alkaline, surfactant and polymer (ASP flooding are very perspective in order to achieve the synergetic effect out of the different impacts which are caused by these chemicals, which affect oil and water filtration in the reservoir and increase oil recovery. In this communication, we consider the applicability of hydrophobically modified polyampholyte – poly(hexadecylaminocrotonatebetaine (PHDACB as ASP flooding agent for recovery of oil from Karazhanbas oilfield. As “polysoap”, the aqueous solution of PHDACB dissolved in aqueous KOH was used. This system combines the advantages of alkaline, surfactant and polymer and exhibits the synergistic effect. The laboratory results showed that the ASP flooding considerably increases the oil recovery in addition to water flooding. In perspective, the ASP flooding may substitute the steam injection and other thermal enhanced oil recovery (EOR technologies.

  16. What Does City Name Split Mean? Etymology of the Ancient Greek Word aspálathos

    Directory of Open Access Journals (Sweden)

    Žarko B. Veljković

    2018-02-01

    Full Text Available The article reviews the current scientific etymology of the city name Split. The latter comes from an Ancient Greek name for the plant aspálathos, that is, ‘Croatian žukovina or brnistra, more accurately, woody shrub Alhagi maurorum, with its flowers of diverse color, from light pink to chestnut, thorny trefoil Calycotome villosa with its light yellow flowers, and the plant Genista acanthoclada with its yellow flowers’. Exploring the etymology of the Ancient Greek name for the plant aspálathos yields the following conclusion: *aspálax or spálax (as name of the plant ‘lily Colchicum parnassicum with its medium violet flowers + suffix -thos > aspálathos’, and that in the general and most common sense: ‘woody shrub Alhagi maurorum, with its flowers of diverse color, from light pink to chestnut’.

  17. Development of alkaline/surfactant/polymer (ASP) flooding technology for recovery of Karazhanbas oil

    OpenAIRE

    Birzhan Zhappasbaev; Iskander Gussenov; Alexey Shakhvorostov; Nurxat Nuraje; Sarkyt Kudaibergenov

    2016-01-01

    The tertiary oil recovery methods like alkaline, surfactant and polymer (ASP) flooding are very perspective in order to achieve the synergetic effect out of the different impacts which are caused by these chemicals, which affect oil and water filtration in the reservoir and increase oil recovery. In this communication, we consider the applicability of hydrophobically modified polyampholyte – poly(hexadecylaminocrotonate)betaine (PHDACB) as ASP flooding agent for recovery of oil from Karazhanb...

  18. Web Development Technology-PHP. How It Is Related To Web Development Technology ASP.NET

    Directory of Open Access Journals (Sweden)

    Manya Sharma

    2015-01-01

    Full Text Available ABSTRACT This paper tells about the technologies used in PHP and how they are related to ASP.NET. The paper begin with the introduction of PHP defining what and how technologies has been used in development of User Complaint Web Application. How thistechnology is related to ASP.NET in features such as implementation functionality validation and proactive behavior involved in validating user input from the browser providing users feedback overall time consumed in development and maintenance.

  19. Localization of tRNAsup(asp)2 genes from Drosophila melanogaster by 'in situ' hybridization

    International Nuclear Information System (INIS)

    Schmidt, T.; Egg, A.H.; Kubli, E.

    1978-01-01

    Transfer RNAsup(asp) 2 delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated 'in vitro' with Na[ 125 I] and hybridized 'in situ' to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAsup(asp) 2 delta to the left arm of the second chromosome in the regions 29 D and E. (orig.) [de

  20. ASP.NET web API build RESTful web applications and services on the .NET framework

    CERN Document Server

    Kanjilal, Joydip

    2013-01-01

    This book is a step-by-step, practical tutorial with a simple approach to help you build RESTful web applications and services on the .NET framework quickly and efficiently.This book is for ASP.NET web developers who want to explore REST-based services with C# 5. This book contains many real-world code examples with explanations whenever necessary. Some experience with C# and ASP.NET 4 is expected.

  1. Characterization of Zinc and Cadmium hyperaccumulation in three Noccaea populations from non-metalliferous sites in the eastern Pyrenees

    Directory of Open Access Journals (Sweden)

    Soledad eMartos

    2016-02-01

    Full Text Available The Southern slope of the Pyrenees is the meridional limit for the distribution of Noccaea populations. However, the systematic description of these populations and their hyperaccumulation mechanisms are not well established. Morphological and genetic analysis (ITS and 3 chloroplast regions were used to identify Noccaea populations localized on non-metallicolous soils during a survey in the Catalonian Pyrenees. Cd and Zn concentrations were analysed in soils and plants both sampled in the field and grown hydroponically. The expression of selected metal transporter genes was assessed by quantitative PCR. The populations were identified as Noccaea brachypetala (Jord. F.K. Mey by conspicuous morphological traits. Principal component analysis provided a clear separation among N. brachypetala, Noccaea caerulescens J. & C. Presl and Noccaea occitanica (Jord. F.K. Mey., three Noccaea species reported in the Pyrenees. Contrastingly, ITS and cpDNA analyses were unable to clearly differentiate these taxa. Differences in the expression of the metal transporter genes HMA3, HMA4, and MTP1 between N. caerulescens and N. brachypetala, and those amongst the N. brachypetala populations suggest differences in the strategies for handling enhanced Cd and Zn availability. This is the first report demonstrating Cd and Zn hyperaccumulation by N. brachypetala both in the field and in hydroponics. This comprehensive study based on taxonomic, molecular, and physiological data allows both the correct identification of this species and the characterization of population differences in hyperaccumulation and tolerance of Zn and Cd.

  2. Application Service Providers (ASP Adoption in Core and Non-Core Functions

    Directory of Open Access Journals (Sweden)

    Aman Y.M. Chan

    2009-10-01

    Full Text Available With the further improvement in internet bandwidth, connection stability and data transmission security, a new wave of Application Service Providers (ASP is on his way. The recent booming on some models such as Software Application as Service (SaaS and On-Demand in 2008, has led to emergence of ASP model in core business functions. The traditional IS outsourcing covers the non-core business functions that are not critical to business performance and competitive advantages. Comparing with traditional IS outsourcing, ASP is a new phenomenon that can be considered as an emerging innovation as it covers both core and non-core business functions. Most of the executives do not comprehend the difference and similarity between traditional IS outsourcing and ASP mode. Hence, we propose to conduct a research so as to identify the determinants (cost benefit, gap in IS capability complementing the company's strategic goal, and trust to ASP's service and security level and moderating factors (management's attitude in ownership & control, and company aggressiveness of ASP adoption decision in both core and non-core business functions.

  3. Large landslides in the Pyrenees: preliminary tasks carried out for a harmonized cross-border risk analysis

    Science.gov (United States)

    Moya, José; Grandjean, Gilles; Copons, Ramon; Vaunat, Jean; Buxó, Pere; Colas, Bastien; Darrozes, José; Gasc, Muriel; Guinau, Marta; Gutiérrez, Francisco; García, Juan Carlos; Virely, Didier; Crosetto, Michele; Mas, Raül

    2017-04-01

    Large landslides are recognised as one of the main erosional agents in mountain ranges, having a significant influence on landscape evolution. However, few efforts have been carried out to assess their geomorphological impact from a regional perspective. Regional-scale investigations are also necessary for the reliable evaluation of the associated risks (i.e. for land-use planning). Large landslides are common in the Pyrenees but: 1) their geographic distribution on a regional scale is not well known; 2) their geological and geomorphological controlling factors have been only studied preliminarily; and 3) their state of activity and stability conditions are unknown for most of the cases. Regional analyses of large landslides, as those carried out by Crosta et al. (2013) in the Alps, are rare worldwide. Jarman et al. (2014) conducted a very preliminary analysis in a sector of the Pyrenees. The construction of a cartographic inventory constitutes the basics for such type of studies, which are typically hindered by the lack of cross-border landslide data bases and methodologies. The aim of this contribution is to present the preliminary works carried out for constructing a harmonized inventory of large landslides in the Pyrenees, involving for the first time both sides of the cordillera and the main groups working in landslide risk in France, Spain and Andorra. Methods used for landslide hazard and risk analysis have been compiled and compared, showing a significant divergence, even as regards the terminology. A preliminary cross-border inventory sheet on risk of large landslides has been prepared. It includes specific fields for the assessment of landslide activity (by using complimentary methods such as morpho-stratigraphy, morphometric analysis and remote techniques) and indirect potential costs (that typically overcome direct ones), which usually are neglected in the existing data bases. Crosta, G.B., Frattini, P. and Agliardi, F., 2013. Deep seated gravitational

  4. Woodland clearance alters geomorphic, hydrologic, and pedogenic drivers of ecosystem services: examples from the southern Blue Ridge (USA) and the French western Pyrenees Mountains

    Science.gov (United States)

    Leigh, David

    2016-04-01

    The southern Blue Ridge (USA) and French western Pyrenees both are humid-temperate mountains where native woodlands have been cleared on soils formed in residuum and colluvium on hillslopes. Forest removal increased rates of erosion and sediment yield that drove both negative and positive ecosystem services. For example, the supportive ecosystem service of soil formation was diminished on eroded hillslopes, but may have been enhanced by accumulation of sediment on bottomlands far downstream from the highland source areas. Negative effects on provisional ecosystem services (e.g. water supply) resulted in aggraded bottomlands by increasing the depth to the water table. Legacy effects linger on hillslopes that reforested (diminished soil properties), and ongoing alteration of pedogenic and hydrologic processes affect pastures that persisted from cleared woodlands. Beyond those general similarities, pastures of the two regions exhibit very different pedogenic pathways and ecosystem service outcomes. Soils of the Blue Ridge pastures adhere to a typical degradation scenario of erosion, compaction, and reduced infiltration capacities, whereas Pyrenees pastures exhibit soil qualities trending in the opposite direction and arguably now are better quality soils than their forested predecessors. Major differences in temporal duration and management styles apparently have led to such contrasts in soil quality. The Blue Ridge pastures are only tens to hundreds of years old, whereas Pyrenees pastures are thousands of years old. Blue Ridge pastures are maintained by mowing with tractors and year-round grazing primarily with beef cattle, whereas Pyrenees pastures (outfields) lack tractors and are only grazed seasonally (summer), primarily with sheep. Fire is rarely used as a management tool in the Blue Ridge, while Pyrenees pastures frequently are burned. Such management practices, and their influence on pedogenic and hydrologic processes, generally have resulted in negative

  5. Impact of topography, climate and moisture sources on isotopic composition (δ18O &δD) of rivers in the Pyrenees: Implications for topographic reconstructions in small orogens

    Science.gov (United States)

    Huyghe, Damien; Mouthereau, Frédéric; Sébilo, Mathieu; Vacherat, Arnaud; Ségalen, Loïc; Richard, Patricia; Biron, Philippe; Bariac, Thierry

    2018-02-01

    Understanding how orogenic topography controls the spatial distribution and isotopic composition of precipitation is critical for paleoaltitudinal reconstructions. Here, we determine the isotopic composition (δ18O and δD) of 82 small rivers and springs from small catchments in the Pyrenees. Calculation of the deuterium excess (d-excess) parameter allows the distinction of four distinct isotopic provinces with d-excess values of between 15 and 22‰ in the northwest, between 7 and 14‰ in the central northern Pyrenees and between 3 and 11‰ in the northeast. The southern Pyrenees have a homogenous d-excess signature ranging from 7 to 14‰. Our results show significant local moisture recycling and/or rain amount effect in the northwestern Pyrenees, and control by evaporation processes during rainfall events in the southern Pyrenees and for low elevated samples of the northeast of the range. Based on the distribution of d-excess values, we estimate contrasting isotope lapse rates of -2.9/-21.4‰/km (δ18O/δD) in the northwest, -2.7/-21.4‰/km (δ18O/δD) in the north central and -3.7/-31.7‰/km (δ18O/δD) in the northeastern Pyrenees. The southern Pyrenees show distinctly higher lapse rates of -9.5/-77.5‰/km (δ18O/δD), indicating that in this area the altitudinal effect in not the only parameter driving isotopic composition of rivers. Despite their relatively low topographic gradient, the Pyrenees exert a direct control on the isotopic composition of river waters, especially on their northern side. The variations in isotopic composition-elevation relationships documented along the strike of the range are interpreted to reflect an increasing continentality effect driven by wind trajectories parallel to the range, and mixing with Mediterranean air masses. Despite these effects, the measurable orographic effect on precipitation in the Pyrenees proves that the isotopic composition approach for reconstructing past topography is applicable to low

  6. Haemoragisk Rift Valley Fever

    DEFF Research Database (Denmark)

    Fabiansen, Christian; Thybo, Søren

    2007-01-01

    A case of fatal hemorrhagic Rift Valley fever during an epidemic in Kenya's North Eastern Province in January 2007 is described.......A case of fatal hemorrhagic Rift Valley fever during an epidemic in Kenya's North Eastern Province in January 2007 is described....

  7. Effect of Asp 96 isomerization on the properties of a lens αB-crystallin-derived short peptide.

    Science.gov (United States)

    Takata, Takumi; Fujii, Noriko

    2015-12-10

    One of the major reasons for age-related cataract formation is an accumulation of insoluble lens proteins. In particular, higher-order α-crystallin aggregates, comprising αA and αB subunits, are insolubilized by the build up of various post-translational modifications over time. Although we previously found an exceptional amount of Asp96 isomerization in αB-crystallin from aged human lens, the biological effect remains unknown. To approximate the effect of Asp 96 isomerization in αB-crystallin, here residues 93-103 of αB-crystallin were chemically synthesized as peptides in which l-α-Asp was replaced with l-β-Asp, D-α-Asp, or D-β-Asp. The resulting peptides were then compared in a biological assay. The results showed that isomerization of Asp 96 altered both the local structure of peptide and its stability against enzymatic digestion. In addition, the synthesized peptides decreased the insoluble fraction of heated α-crystallin. The D-β-Asp-containing peptide further decreased heat-induced precipitation of α-crystallin, and a chaperone assay based on heated alcohol dehydrogenase implied differential interaction of the peptides with substrate depending on the Asp isomer present in each. Our results suggest that the formation of Asp isomers is likely to affect the higher-order oligomer structure of α-crystallin and thereby its chaperone functions in aged lens. Copyright © 2015 Elsevier B.V. All rights reserved.

  8. Dermatophytosis caused by Trichophyton mentagrophytes in the southern chamois (Rupicapra pyrenaica) in the Eastern Pyrenees.

    Science.gov (United States)

    Marco, I; Lopez-Olvera, J R; Gibert, P; Abarca, L; Gauthier, D; Lavín, S

    2007-01-01

    Two free-ranging southern chamois (Rupicapra pyrenaica) - one 4-year-old male and one 3-year-old male - were diagnosed with clinical dermatophytosis in the French and Spanish Eastern Pyrenees respectively. Skin samples were collected for microbiological studies, Trichophyton mentagrophytes being isolated and identified in both animals. The first chamois was found dead at the base of a cliff, and presented with alopecia and scaling on the dorsum and left forelimb. The second chamois showed grey-yellow, rough to raised scaling and crusting skin lesions at the base of the horns, around the eyes, dorsum of the nose, sternum, tail and limbs. Histological examination was carried out only on the second animal. The main lesions were orthokeratotic hyperkeratosis with focal parakeratosis, irregular to papillary epidermal hyperplasia, intracorneal neutrophilic pustules, perivascular to diffuse dermatitis with neutrophilic folliculitis and furunculosis. In those follicles with folliculitis and/or furunculosis, fungal hyphae and arthrospores associated with the follicular keratin and hair shafts were seen.

  9. Climate Change and Policy Effects: The Development of Renewable Energy in the Midi-Pyrenees

    International Nuclear Information System (INIS)

    Behar, Laurie; Leroy, Pieter

    2014-01-01

    In order to analyze the construction of regional-scale policies for fighting climate change, we have chosen to study windmill development, wood energy and solar power in the region of Midi-Pyrenees. More precisely, we study the interactions between public actors, local associations and renewable energy experts, the manner in which this interface has been institutionalized and what this process means in terms of knowledge acquisition among the actors involved. Theoretically, we seek to shed light on neo-institutionalist and sociology of science approaches. By drawing upon the 'policy arrangements' framework of analysis, we show that three of the dimensions characterizing the policy arrangement of renewable energy - coalitions among actors, the distribution of resources and the legal framework - have gradually been modified. This has led to changes in a fourth dimension - that of dominant discourse. Finally, even if interactions between experts and local associations may play a decisive role in the initial phase of the construction of energy policies, public actors gradually succeed in bringing the implementation of these policies under their control

  10. Beef animal welfare, attitudes and Willingness to Pay: A regional comparison across the Pyrenees

    Energy Technology Data Exchange (ETDEWEB)

    Sans, P.; Sanjuán-López, A.I.

    2015-07-01

    Attitudes towards beef animal welfare (AW) and Willingness to Pay (WTP) for AW certification are investigated among consumers in two Spanish and two French regions located on both sides of the Pyrenees (n=1213). Attitudes were measured through a scale of 11 animal practices, on which, consumers report their degree of concern and trust on the supply chain compliance. Attitudes significantly differed across regions, especially with respect to those AW practices carried out by farmers, while trust lies behind concerns. Three segments based on individual consumer attitudes are defined by opposing those consumers who are more concerned and who trust more on the compliance with AW standards (n=264, 22%) to those less concerned and who are more uncertain about stakeholders´ compliance with AW rules (n=356, 29%). Consumer location, gender, age and education significantly differed across attitudinal clusters. Results from a contingent valuation survey show that WTP for certified animal friendly beef ranged between 20.6% and 22.6% over the average market price of standard beef, in Spain and France, respectively. Both, consumers’ socio-demographic characteristics and habits regarding beef meat purchasing and attitudes towards farmers influenced this WTP (the more consumers trust in farmers’ involvement in animal welfare, the highest is their WTP), while a negative overall attitude significantly reduced WTP. (Author)

  11. Beef animal welfare, attitudes and Willingness to Pay: A regional comparison across the Pyrenees

    Directory of Open Access Journals (Sweden)

    Pierre Sans

    2015-09-01

    Full Text Available Attitudes towards beef animal welfare (AW and Willingness to Pay (WTP for AW certification are investigated among consumers in two Spanish and two French regions located on both sides of the Pyrenees (n=1213. Attitudes were measured through a scale of 11 animal practices, on which, consumers report their degree of concern and trust on the supply chain compliance. Attitudes significantly differed across regions, especially with respect to those AW practices carried out by farmers, while trust lies behind concerns. Three segments based on individual consumer attitudes are defined by opposing those consumers who are more concerned and who trust more on the compliance with AW standards (n=264, 22% to those less concerned and who are more uncertain about stakeholders´ compliance with AW rules (n=356, 29%. Consumer location, gender, age and education significantly differed across attitudinal clusters. Results from a contingent valuation survey show that WTP for certified animal friendly beef ranged between 20.6% and 22.6% over the average market price of standard beef, in Spain and France, respectively. Both, consumers’ socio-demographic characteristics and habits regarding beef meat purchasing and attitudes towards farmers influenced this WTP (the more consumers trust in farmers’ involvement in animal welfare, the highest is their WTP, while a negative overall attitude significantly reduced WTP.

  12. A multi-proxy perspective on millennium-long climate variability in the Southern Pyrenees

    Science.gov (United States)

    Morellón, M.; Pérez-Sanz, A.; Corella, J. P.; Büntgen, U.; Catalán, J.; González-Sampériz, P.; González-Trueba, J. J.; López-Sáez, J. A.; Moreno, A.; Pla-Rabes, S.; Saz-Sánchez, M. Á.; Scussolini, P.; Serrano, E.; Steinhilber, F.; Stefanova, V.; Vegas-Vilarrúbia, T.; Valero-Garcés, B.

    2012-03-01

    This paper reviews multi-proxy paleoclimatic reconstructions with robust age-control derived from lacustrine, dendrochronological and geomorphological records and characterizes the main environmental changes that occurred in the Southern Pyrenees during the last millennium. Warmer and relatively arid conditions prevailed during the Medieval Climate Anomaly (MCA, ca. 900-1300 AD), with a significant development of xerophytes and Mediterranean vegetation and limited deciduous tree formations (mesophytes). The Little Ice Age (LIA, 1300-1800 AD) was generally colder and moister, with an expansion of deciduous taxa and cold-adapted montane conifers. Two major phases occurred within this period: (i) a transition MCA-LIA, characterized by fluctuating, moist conditions and relatively cold temperatures (ca. 1300 and 1600 AD); and (ii) a second period, characterized by the coldest and most humid conditions, coinciding with maximum (recent) glacier advances (ca. 1600-1800 AD). Glaciers retreated after the LIA when warmer and more arid conditions dominated, interrupted by a short-living cooling episode during the late 19th to early 20th centuries. Some records suggest a response to solar activity with colder and slightly moister conditions during solar minima. Centennial-scale hydrological fluctuations are in phase with reconstructions of NAO variability, which appears to be one of the main climate mechanisms influencing rainfall variations in the region during the last millennium.

  13. A multi-proxy perspective on millennium-long climate variability in the Southern Pyrenees

    Directory of Open Access Journals (Sweden)

    M. Morellón

    2012-03-01

    Full Text Available This paper reviews multi-proxy paleoclimatic reconstructions with robust age-control derived from lacustrine, dendrochronological and geomorphological records and characterizes the main environmental changes that occurred in the Southern Pyrenees during the last millennium. Warmer and relatively arid conditions prevailed during the Medieval Climate Anomaly (MCA, ca. 900–1300 AD, with a significant development of xerophytes and Mediterranean vegetation and limited deciduous tree formations (mesophytes. The Little Ice Age (LIA, 1300–1800 AD was generally colder and moister, with an expansion of deciduous taxa and cold-adapted montane conifers. Two major phases occurred within this period: (i a transition MCA–LIA, characterized by fluctuating, moist conditions and relatively cold temperatures (ca. 1300 and 1600 AD; and (ii a second period, characterized by the coldest and most humid conditions, coinciding with maximum (recent glacier advances (ca. 1600–1800 AD. Glaciers retreated after the LIA when warmer and more arid conditions dominated, interrupted by a short-living cooling episode during the late 19th to early 20th centuries. Some records suggest a response to solar activity with colder and slightly moister conditions during solar minima. Centennial-scale hydrological fluctuations are in phase with reconstructions of NAO variability, which appears to be one of the main climate mechanisms influencing rainfall variations in the region during the last millennium.

  14. Role of Asp544 in subunit I for Na+ pumping by Vitreoscilla cytochrome bo

    International Nuclear Information System (INIS)

    Chung, Yeon T.; Stark, Benjamin C.; Webster, Dale A.

    2006-01-01

    The conserved Glu540 in subunit I of Escherichia coli cytochrome bo (a H + pump) is replaced by Asp544 in the Vitreoscilla enzyme (a Na + pump). Site-directed mutagenesis of the Vitreoscilla cytochrome bo operon changed this Asp to Glu, and both wild type and mutant cyo's were transformed into E. coli strain GV100, which lacks cytochrome bo. Compared to the wild type transformant the Asp544Glu transformant had decreased ability to pump Na + as well as decreased stimulation in respiratory activity in the presence of Na + . Preliminary experiments indicated that this mutant also had increased ability to pump protons, suggesting that this single change may provide cation pumping specificity in this group of enzymes

  15. Analisis Perancangan Customer Relationship Management Berbasis Web pada PT ASP Jakarta

    Directory of Open Access Journals (Sweden)

    Idris Gautama So

    2011-05-01

    Full Text Available The purpose of this thesis is to identify the needs of web-based CRM in PT ASP and also to design the suitable CRM for the company. The research method is by analyzing competitions in the industry with Porter’s five forces analysis and formulating framework in input stage, matching stage, decision stage to choose the best strategy for the company. From the analysis of formulating strategy, it could be concluded that market penetration strategy is the best strategy for PT ASP. Market penetration strategy is an approach of web-based Customer Relationship Management (CRM to increase service quality for customers and company introduction to broaden community. Web-based CRM design is suitable for PT ASP based on interview conclusion using 5 features (5C from the 7 features (7C in web design elements. 

  16. A tonoplast Glu/Asp/GABA exchanger that affects tomato fruit amino acid composition.

    Science.gov (United States)

    Snowden, Christopher J; Thomas, Benjamin; Baxter, Charles J; Smith, J Andrew C; Sweetlove, Lee J

    2015-03-01

    Vacuolar accumulation of acidic metabolites is an important aspect of tomato fruit flavour and nutritional quality. The amino acids Asp and Glu accumulate to high concentrations during ripening, while γ-aminobutyrate (GABA) shows an approximately stoichiometric decline. Given that GABA can be catabolised to form Glu and subsequently Asp, and the requirement for the fruit to maintain osmotic homeostasis during ripening, we hypothesised the existence of a tonoplast transporter that exports GABA from the vacuole in exchange for import of either Asp or Glu. We show here that the tomato vacuolar membrane possesses such a transport property: transport of Glu across isolated tonoplast vesicle membranes was trans-stimulated in counterexchange mode by GABA, Glu and Asp. We identified SlCAT9 as a candidate protein for this exchanger using quantitative proteomics of a tonoplast-enriched membrane fraction. Transient expression of a SlCAT9-YFP fusion in tobacco confirmed a tonoplast localisation. The function of the protein was examined by overexpression of SlCAT9 in transgenic tomato plants. Tonoplast vesicles isolated from transgenic plants showed higher rates of Glu and GABA transport than wild-type (WT) only when assayed in counterexchange mode with Glu, Asp, or GABA. Moreover, there were substantial increases in the content of all three cognate amino acids in ripe fruit from the transgenic plants. We conclude that SlCAT9 is a tonoplast Glu/Asp/GABA exchanger that strongly influences the accumulation of these amino acids during fruit development. © 2015 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

  17. Introduction of the ASP3D Computer Program for Unsteady Aerodynamic and Aeroelastic Analyses

    Science.gov (United States)

    Batina, John T.

    2005-01-01

    A new computer program has been developed called ASP3D (Advanced Small Perturbation 3D), which solves the small perturbation potential flow equation in an advanced form including mass-consistent surface and trailing wake boundary conditions, and entropy, vorticity, and viscous effects. The purpose of the program is for unsteady aerodynamic and aeroelastic analyses, especially in the nonlinear transonic flight regime. The program exploits the simplicity of stationary Cartesian meshes with the movement or deformation of the configuration under consideration incorporated into the solution algorithm through a planar surface boundary condition. The new ASP3D code is the result of a decade of developmental work on improvements to the small perturbation formulation, performed while the author was employed as a Senior Research Scientist in the Configuration Aerodynamics Branch at the NASA Langley Research Center. The ASP3D code is a significant improvement to the state-of-the-art for transonic aeroelastic analyses over the CAP-TSD code (Computational Aeroelasticity Program Transonic Small Disturbance), which was developed principally by the author in the mid-1980s. The author is in a unique position as the developer of both computer programs to compare, contrast, and ultimately make conclusions regarding the underlying formulations and utility of each code. The paper describes the salient features of the ASP3D code including the rationale for improvements in comparison with CAP-TSD. Numerous results are presented to demonstrate the ASP3D capability. The general conclusion is that the new ASP3D capability is superior to the older CAP-TSD code because of the myriad improvements developed and incorporated.

  18. Building a dynamically ASP.NET 2.0 GridView control

    Directory of Open Access Journals (Sweden)

    Catalin NACHILA

    2008-01-01

    Full Text Available Microsoft Visual Studio 2005 (based on ASP.NET 2.0, the successor to Visual Studio .NET 2003 has a lot of new features and goodies designed for Web developers. This article show how a ASP.NET 2.0 control can be dynamically connected to Microsoft Access database. The delete and update operation will be implemented using a GridView control and SQL queries. The connection between the database and the .NET application will be made with OleDb Data provider, the new Access Data Source control. The SQL queries will be implemented with OleDbCommand.

  19. C# 4, ASP.NET 4, and WPF, with Visual Studio 2010 jump start

    CERN Document Server

    Nagel, Christian; Stephens, Rod; Hanselman, Scott; Glynn, Jay; Rader, Devin; Watson, Karli; Skinner, Morgan

    2010-01-01

    This Wrox Blox is a value-packed resource to help experienced .NETdevelopers learn the new .NET release. It is excerpted from theWrox books: Professional C# 4 and .NET 4, Professional ASP.NET4, and WPF Programmer's Reference by Christian Nagel,Bill Evjen, Scott Hanselman, and Rod Stephens, and includes morethan 100 print book pages drawn from these three key titles. It isan excellent resource to help .NET developers get up to speed faston .NET 4, C# 4.0, ASP.NET 4, and WPF, providing all theinformation needed to program with the important new features,including: C# Dynamic Types and Par

  20. ASP.NET MVC 4 recipes a problem-solution approach

    CERN Document Server

    Ciliberti, John

    2013-01-01

    ASP.NET MVC 4 Recipes is a practical guide for developers creating modern web applications, cutting through the complexities of ASP.NET, jQuery, Knockout.js and HTML 5 to provide straightforward solutions to common web development problems using proven methods based on best practices. The problem-solution approach gets you in, out, and back to work quickly while deepening your understanding of the underlying platform and how to develop with it. Author John Ciliberti guides you through the framework and development tools, presenting typical challenges, along with code solutions and clear, conci

  1. What Does City Name Split Mean? Etymology of the Ancient Greek Word aspálathos

    OpenAIRE

    Žarko B. Veljković

    2018-01-01

    The article reviews the current scientific etymology of the city name Split. The latter comes from an Ancient Greek name for the plant aspálathos, that is, ‘Croatian žukovina or brnistra, more accurately, woody shrub Alhagi maurorum, with its flowers of diverse color, from light pink to chestnut, thorny trefoil Calycotome villosa with its light yellow flowers, and the plant Genista acanthoclada with its yellow flowers’. Exploring the etymology of the Ancient Greek name for the plant aspálatho...

  2. Immediate changes in topsoil chemical properties after controlled shrubland burning in the Central Pyrenees

    Science.gov (United States)

    Zufiaurre-Galarza, Raquel; Fernández Campos, Marta; Badía-Villas, David; María Armas-Herrera, Cecilia; Martí-Dalmau, Clara; Girona-García, Antonio

    2016-04-01

    Prescribed fire has recently been adopted as an encroachment-fighting strategy in the Central Pyrenees. Despite relatively large information on wildfire impacts on soil, there is little information on prescribed fire effects, especially in mountain ecosystems (Shakesby et al, 2015). Fire effects are noticeable in the topsoil, particularly in relation to soil organic matter and nutrient contents and quality (Alexis et al, 2012). These components change with time after fire and at the scale of the upper few centimetres of mineral soil (Badía et al, 2014). The aim of this study is to evaluate the immediate effects of prescribed shrubland burning on soil's nutrients and organic matter content to detect changes at cm-scale, trying to differentiate the heat shock from the subsequent incorporation of ash and charcoal. The study area, densely covered with spiny broom (Echinospartum horridum), is located in Tella (Central Pyrenees, NE Spain) at 1900 meters above sea level. Three sites were sampled before burning and immediately after burning just in its adjacent side. The soils belong to the WRB unit Leptic Eutric Cambisol, Soil samples were collected separating carefully the organic layers (litter in unburned soils and ashes and fire-altered organic residues in burned soils) and the mineral horizon at 0-1, 1-2 and 2-3 cm depths. Soil samples were air-dried and sieved to 2 mm. Soil organic C (by the wet oxidation method), total N (Kjeldahl method), water-soluble ions (Ca2+, Mg2+, Na+, K+, SO4=, NO3- and NH4+), exchangeable ions (Ca2+, Mg2+, K+, Na+, Fe3+ and Mn2+), total and available P, pH (1:5) and the electrical conductivity (in a 1:10 soil-to-water ratio) were measured. Immediately after the controlled fire, soil organic carbon content on burned topsoil decreases significantly within 0-3 cm of soil depth studied while total N decrease was not significant. Moreover, only a slight increase of the electrical conductivity, water-soluble ions and exchangeable ions was

  3. CYP2C9 polymorphism in five autochthonous population of the same geographic area (Spanish Pyrenees).

    Science.gov (United States)

    Borobia, Alberto M; López-Parra, Ana María; Tabarés, Beatriz; Ramirez, Elena; Baeza, Carlos; Arroyo-Pardo, Eduardo; Carcas, Antonio J

    2009-02-01

    To evaluate the frequency of CYP2C9 polymorphisms in a cohort of Caucasians (Spanish Pyrenees), previously classified in autochthonous populations. Blood samples from 154 anonymous volunteer donors were collected. All the individuals were autochthonous to their respective populations (four grandparents born in the region): 23 from Valle de Arán (Lérida), 29 from Alto Urgel (Lérida), 32 from La Cerdaña (Gerona), 30 from Jacetania (Huesca) and 40 from Cinco Villas (Navarra). The analyses for allelic mutation, CYP2C9*2 and CYP2C9*3, were identified with Taqman Allelic Discrimination kits. No statistical differences were found when allelic frequencies in the five autochthonous populations were compared. Frequency distribution of genotypic classes (wt/wt, wt/mut and mut/mut) in Alto Urgel was different from that in La Cerdaña, Cinco Villas and Jacetania samples. Comparison of Pyrenean and other European populations through exact test revealed significant differences in the distribution of genotypic classes: Alto Urgel, Barcelona, and Croatia yielded the highest significant differences. According to the exact test these populations were pooled in four groups. This classification produced a statistically significant percentage of variation explained by differences among groups (1.94%, P= 0.036), but not by differences among populations within groups (P=0.914), although most of the percentage of variance is explained by differences within populations (97.46%, P<0.001). This study increases the evidence of intra-population genotypic variability and highlights the significant genotypic heterogeneity when different autochthonous populations are considered, despite no clear differences in allelic frequencies do exist.

  4. Spatial distribution and environmental analysis of the alpine flora in the Pyrenees

    Directory of Open Access Journals (Sweden)

    D. Gómez

    2017-09-01

    Full Text Available On the basis of the digital edition of the “Atlas of the vascular flora of the Pyrenees” (www.florapyrenaea. org, the alpine flora of this mountain range is delimited in order to know its diversity and the different patterns of its spatial distribution, along with some other environmental characteristics. The Pyrenean alpine flora is made up of 645 taxa (630 species and 15 subspecies. All the administrative regions harbour more than 60% of the alpine plants, with Catalonia and Aragon reaching the highest values (around 90%. Along the altitudinal gradient, the highest plant diversity is found between 2300 and 2600 m. a. s. l., although 25% of the total alpine flora goes beyond 3000 m. On the other hand, a remarkable number of alpine plants live in the lowlands, and thus more than 300 alpine plants can be found below 1500 m. The average altitude range of the alpine plants is 1369 m, 300 m wider than that observed for the whole Pyrenean flora. Life-forms, habitat distribution and habitat naturalness of alpine plants are significantly different from those of the whole Pyrenean flora. Distribution of abundance categories also shows values of rarity significantly lower among alpine plants than for the whole flora. More than half the Pyrenean endemic plants are present in the alpine flora. High diversity and wide ecological amplitude of the alpine flora must be taken into account either when considering vulnerability of alpine plants facing “global change” or when addressing conservation policies for the whole Pyrenees from a common perspective.

  5. Modelling the impact of forest loss on shallow landslide sediment yield, Ijuez river catchment, Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    2007-01-01

    Full Text Available The SHETRAN model for simulating the sediment yield arising from shallow landslides at the scale of a river catchment was applied to the 45-km2 Ijuez catchment in the central Spanish Pyrenees, to investigate the effect of loss of forest cover on landslide and debris flow incidence and on catchment sediment yield. The application demonstrated how such a model, with a large number of parameters to be evaluated, can be used even when directly measured data are not available: rainfall and discharge time series were generated by reference to other local records and data providing the basis for a soil map were obtained by a short field campaign. Uncertainty bounds for the outputs were determined as a function of the uncertainty in the values of key model parameters. For a four-year period and for the existing forested state of the catchment, a good ability to simulate the observed long term spatial distribution of debris flows (represented by a 45-year inventory and to determine catchment sediment yield within the range of regional observations was demonstrated. The lower uncertainty bound on simulated landslide occurrence approximated the observed annual rate of landsliding and suggests that landslides provide a relatively minor proportion of the total sediment yield, at least in drier years. A scenario simulation in which the forest cover was replaced by grassland indicated an increase in landsliding but a decrease in the number of landslides which evolve into debris flows and, at least for drier years, a reduction in sediment delivery to the channel network.

  6. Spatial and temporal variation in snow accumulation in the Central Spanish Pyrenees

    Science.gov (United States)

    López-Moreno, I.; Beguería, S.; García-Ruiz, J. M.

    2003-04-01

    Water stored in winter snowpack represents a valuable resource in mountainous regions. The distribution of the snow determines the availability of water resources during snowmelt period, as well as the development of the economy based on winter sports. This work analyses the main factors that explain the variation in snow accumulation in the Central Spanish Pyrenees. Data about evolution of snowpack is provided by the measurement of 106 sticks installed in the study area from 1985. The snow depth is measured in two moments of the year, at the beginning of March and at the end of April or beginning of May. Snow depth, of both measurements moments, has been mapped using linear regression between snow depth and different topographic and geographic variables derived from the digital terrain model. In order to improve the estimation interpolated residuals values have been subtracted. The variability of the interannual snow distribution has been analysed with a factorial analysis in order to obtain different annual patterns of snowpack distribution. Relation between the dominant winter weather-type and the different patterns, provided by the factorial analysis, has been studied. The weather-type classification has been obtained using the Jenkinson and Collison system based on daily series of sea level atmospheric pressure measured around the Iberian Peninsula with a 5º x 10º scale. Thus, it is possible to know the influence of the prevailing winter synoptic situations in the snowpack distribution. The results show that topographic and geographic variables have a high capacity to explain the spatial distribution of the average snow cover during the study period. Nevertheless, the annual snow accumulation is the result of the arrival of frontal disturbances that come from different directions. By that, it is complex to relate the different snow distribution patterns to the main winter synoptic conditions of each year.

  7. Forecasting and modelling ice layer formation on the snowpack due to freezing precipitations in the Pyrenees

    Science.gov (United States)

    Quéno, Louis; Vionnet, Vincent; Cabot, Frédéric; Vrécourt, Dominique; Dombrowski-Etchevers, Ingrid

    2017-04-01

    In the Pyrenees, freezing precipitations in altitude occur at least once per winter, leading to the formation of a pure ice layer on the surface of the snowpack. It may lead to accidents and fatalities among mountaineers and skiers, with sometimes a higher human toll than avalanches. Such events are not predicted by the current operational systems for snow and avalanche hazard forecasting. A crowd-sourced database of surface ice layer occurrences is first built up, using reports from Internet mountaineering and ski-touring communities, to mitigate the lack of observations from conventional observation networks. A simple diagnostic of freezing precipitation is then developed, based on the cloud water content and screen temperature forecast by the Numerical Weather Prediction model AROME, operating at 2.5-km resolution. The performance of this diagnostic is assessed for the event of 5-6 January 2012, with a good representation of altitudinal and spatial distributions of the ice layer. An evaluation of the diagnostic for major events over five winters gives good skills of detection compared to the occurrences reported in the observation database. A new modelling of ice formation on the surface of the snowpack due to impinging supercooled water is added to the detailed snowpack model Crocus. It is combined to the atmospheric diagnostic of freezing precipitations and resulting snowpack simulations over a winter season capture well the formation of the main ice layers. Their influence on the snowpack stratigraphy is also realistically simulated. These simple methods enable to forecast the occurrence of surface ice layer formations with good confidence and to simulate their evolution within the snowpack, even if an accurate estimation of freezing precipitation amounts remains the main challenge.

  8. The fluvial sediment budget of a dammed river (upper Muga, southern Pyrenees)

    Science.gov (United States)

    Piqué, G.; Batalla, R. J.; López, R.; Sabater, S.

    2017-09-01

    Many rivers in the Mediterranean region are regulated for urban and agricultural purposes. Reservoir presence and operation results in flow alteration and sediment discontinuity, altering the longitudinal structure of the fluvial system. This study presents a 3-year sediment budget of a highly dammed Mediterranean river (the Muga, southern Pyrenees), which has experienced flow regulation since the 1969 owing to a 61-hm3 reservoir. Flow discharge and suspended sediment concentration were monitored immediately upstream and downstream from the reservoir, whereas bedload transport was estimated by means of bedload formulae and estimated from regional data. Results show how the dam modifies river flow, reducing the magnitude of floods and shortening its duration. At the same time, duration of low flows increases. The downstream flow regime follows reservoir releases that are mostly driven by the irrigation needs in the lowlands. Likewise, suspended sediment and bedload transport are shown to be notably affected by the dam. Sediment transport upstream was mainly associated with floods and was therefore concentrated in short periods of time (i.e., > 90% of the sediment load occurred in transported more constantly (i.e., 90% of the load was carried during 50% of the time). Total sediment load upstream from the dam equalled 23,074 t, while downstream it was transport downstream. More than 95% of the sediments transported from the upstream basins were trapped in the reservoir, a fact that explains the sediment deficit and the river bed armouring observed downstream. Overall, the dam disrupted the natural water and sediment fluxes, generating a highly modified environment downstream. Below the dam, the whole ecosystem shifted to stable conditions owing to the reduction of water and sediment loads.

  9. Perception of teratogenic and foetotoxic risk by health professionals: a survey in Midi-Pyrenees area.

    Directory of Open Access Journals (Sweden)

    Damase-Michel C

    2008-03-01

    Full Text Available Counselling or prescribing drugs during pregnancy requires health professionals to assess risk/benefit ratio for women and their baby. A misperception of the risk may lead to inappropriate decisions for pregnancy outcomes. The aim of the present study was to assess teratogenic and/or foetotoxic risk perception of common medications by general practitioners (GPs and community pharmacists (CPs from the Midi-Pyrenees area.Methods: 103 GPs and 104 CPs were interviewed. For 21 given drugs, a visual-analogue scale was used to evaluate the risk to give birth to a malformed infant if the mother had taken the drug during first trimester of pregnancy. For 9 drugs, health professionals had to say if they thought there was a potential foetotoxic and/or neonatal risk when drugs were administered during late pregnancy.Results: 97% and 91% of GPs and CPs respectively thought that isotretinoin and thalidomide are teratogenic and more than 80% thought that amoxicillin and acetaminophen are safe in early pregnancy. However, 19% of the GPs and 33% of CPs answered there were no teratogenic risk for valproate. Around 11% of both GPs and CPs said that warfarin was safe during pregnancy. For 22% of GPs and for 13% and 27% of CPs respectively, ibuprofen and enalapril were safe on late pregnancy. For each drug, mean value of perceived teratogenic risk by health professionals was higher than values that can be found in scientific references. Concerning isotretinoin, thalidomide and metoclopramide, perceived teratogenic risk was higher for CPs.Conclusion: These data show that the potential teratogenic and foetotoxic risk of several commonly used drugs is unknown by health professionals. Conversely, GPs and CPs who think that a risk exists, overestimate it. This misperception can lead to inappropriate decisions for pregnancy outcomes.

  10. Determination of the protonation state of the Asp dyad: conventional molecular dynamics versus thermodynamic integration.

    Science.gov (United States)

    Huang, Jinfeng; Zhu, Yali; Sun, Bin; Yao, Yuan; Liu, Junjun

    2016-03-01

    The protonation state of the Asp dyad is important as it can reveal enzymatic mechanisms, and the information this provides can be used in the development of drugs for proteins such as memapsin 2 (BACE-1), HIV-1 protease, and rennin. Conventional molecular dynamics (MD) simulations have been successfully used to determine the preferred protonation state of the Asp dyad. In the present work, we demonstrate that the results obtained from conventional MD simulations can be greatly influenced by the particular force field applied or the values used for control parameters. In principle, free-energy changes between possible protonation states can be used to determine the protonation state. We show that protonation state prediction by the thermodynamic integration (TI) method is insensitive to force field version or to the cutoff for calculating nonbonded interactions (a control parameter). In the present study, the protonation state of the Asp dyad predicted by TI calculations was the same regardless of the force field and cutoff value applied. Contrary to the intuition that conventional MD is more efficient, our results clearly show that the TI method is actually more efficient and more reliable for determining the protonation state of the Asp dyad.

  11. The role of Arg445 and Asp498 in the human thyroid hormone transporter MCT8

    NARCIS (Netherlands)

    S. Groeneweg (Stefan); E.C.H. Friesema (Edith); S. Kersseboom (Simone); W. Klootwijk (Willem); W.E. Visser (Wil Edward); R.P. Peeters (Robin); T.J. Visser (Theo)

    2014-01-01

    textabstractMonocarboxylate transporter 8 (MCT8) facilitates cellular influx and efflux of the thyroid hormones (THs) T4 and T3. Mutations in MCT8 lead to severe psychomotor retardation. Here, we studied the importance of 2 highly conserved residues (Arg445 in transmembrane domain 8 and Asp498 in

  12. Usage Of Asp.Net Ajax for Binus School Serpong Web Applications

    Directory of Open Access Journals (Sweden)

    Karto Iskandar

    2016-03-01

    Full Text Available Today web applications have become a necessity and many companies use them as a communication tool to keep in touch with their customers. The usage of Web Application in current time increases as the numberof internet users has been rised. For reason of Rich Internet Application, the desktop application developer wasmoved to web application developer with AJAX technology. BINUS School Serpong is a Cambridge Curriculum base International School that uses web application for access every information about the school. By usingAJAX, performance of web application should be improved and the bandwidth usage is decreased. Problems thatoccur at BINUS School Serpong is not all part of the web application that uses AJAX. This paper introducesusage of AJAX in ASP.NET with C# programming language in web application BINUS School Serpong. It is expected by using ASP.NET AJAX, BINUS School Serpong website performance will be faster because of reducing web page reload. The methodology used in this paper is literature study. Results from this study are to prove that the ASP.NET AJAX can be used easily and improve BINUS School Serpong website performance. Conclusion of this paper is the implementation of ASP.NET AJAX improves performance of web application in BINUS School Serpong.

  13. Gly118Asp is a SCA14 founder mutation in the Dutch ataxia population

    NARCIS (Netherlands)

    Verbeek, DS; van de Warrenburg, BPC; Hennekam, FAM; Dooijes, D; Ippel, PF; Verschuuren-Bemelmans, CC; Kremer, HPH; Sinke, RJ

    Missense mutations in the PRKCG gene have recently been identified in spinocerebellar ataxia 14 (SCA14) patients; these include the Gly118Asp mutation that we found in a large Dutch autosomal dominant cerebellar ataxia (ADCA) family. We subsequently screened the current Dutch ataxia cohort

  14. The Academic Support Process (ASP) website: helping preceptors develop resident learning plans and track progress.

    Science.gov (United States)

    Stodel, Emma J; Montpetit, Madeleine; Eyre, Alison; Prentice, Michelle; Johnston, Mary

    2012-01-01

    At times, preceptors struggle with aspects of resident education. Many are looking for more support and faculty development in this area. To address preceptors' needs for resources and provide a proactive framework for their teaching, the Academic Support Process (ASP) website was developed and evaluated. Preceptors' (N = 35) experiences using the ASP website, as well as their perceptions of its usefulness in supporting resident education, were identified. The research comprised two phases: a self-directed workshop involving the creation of a web-based learning plan for a standardised scenario of a resident in difficulty followed by 3 months use of the ASP website with residents in their practice. Information on their experiences was solicited via surveys and focus group interviews. Findings revealed the ASP website enabled preceptors to find words for their concerns around resident competency, gave them a proactive teaching framework, expanded their arsenal of teaching strategies, and supported a customised approach for all learners along the performance spectrum. However, there were a number of challenges encountered by the preceptors that affected site use and buy in. Results are promising. Next steps involve developing a clear strategy for adoption.

  15. Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI.

    Science.gov (United States)

    Horton, John R; Nugent, Rebecca L; Li, Andrew; Mabuchi, Megumu Yamada; Fomenkov, Alexey; Cohen-Karni, Devora; Griggs, Rose M; Zhang, Xing; Wilson, Geoffrey G; Zheng, Yu; Xu, Shuang-yong; Cheng, Xiaodong

    2014-03-07

    The modification-dependent restriction endonuclease AspBHI recognizes 5-methylcytosine (5mC) in the double-strand DNA sequence context of (C/T)(C/G)(5mC)N(C/G) (N = any nucleotide) and cleaves the two strands a fixed distance (N12/N16) 3' to the modified cytosine. We determined the crystal structure of the homo-tetrameric AspBHI. Each subunit of the protein comprises two domains: an N-terminal DNA-recognition domain and a C-terminal DNA cleavage domain. The N-terminal domain is structurally similar to the eukaryotic SET and RING-associated (SRA) domain, which is known to bind to a hemi-methylated CpG dinucleotide. The C-terminal domain is structurally similar to classic Type II restriction enzymes and contains the endonuclease catalytic-site motif of DX20EAK. To understand how specific amino acids affect AspBHI recognition preference, we generated a homology model of the AspBHI-DNA complex, and probed the importance of individual amino acids by mutagenesis. Ser41 and Arg42 are predicted to be located in the DNA minor groove 5' to the modified cytosine. Substitution of Ser41 with alanine (S41A) and cysteine (S41C) resulted in mutants with altered cleavage activity. All 19 Arg42 variants resulted in loss of endonuclease activity.

  16. Stability of Asp(B28) Insulin Exposed to Modified and Unmodified Polypropylene

    DEFF Research Database (Denmark)

    Fristrup, C. J.; Jankova, K.; Eskimergen, R.

    2015-01-01

    Polypropylene (PP) plates have been modified with two different hydrophilic polymeric materials, poly(N,N-dimethylacrylamide) (poly(DMAAm)) and poly(poly(ethylene glycol)methacrylate) (poly(PEGMA)) in order to reduce insulin adsorption when the plates were exposed to insulin aspart (Asp(B28...

  17. Activated sludge process simulator ASP-Sim, Part-2: Bod removal ...

    African Journals Online (AJOL)

    The software was validated by comparing its output with design problems in literature. The memory space requirement for the simulator is 392kb with an average execution time per option of about 1.0s. Results obtained proves Visual Basic 6.0 program (ASP-SIM) a useful tool for wastewater treatment plant design and ...

  18. The FlatModel: a 2D numerical code to evaluate debris flow dynamics. Eastern Pyrenees basins application.

    Science.gov (United States)

    Bateman, A.; Medina, V.; Hürlimann, M.

    2009-04-01

    Debris flows are present in every country where a combination of high mountains and flash floods exists. In the northern part of the Iberian Peninsula, at the Pyrenees, sporadic debris events occur. We selected two different events. The first one was triggered at La Guingueta by the big exceptional flood event that produced many debris flows in 1982 which were spread all over the Catalonian Pyrenees. The second, more local event occurred in 2000 at the mountain Montserrat at the Pre-litoral mountain chain. We present here some results of the FLATModel, entirely developed at the Research Group in Sediment Transport of the Hydraulic, Marine and Environmental Engineering Department (GITS-UPC). The 2D FLATModel is a Finite Volume method that uses the Godunov scheme. Some numerical arranges have been made to analyze the entrainment process during the events, the Stop & Go phenomena and the final deposit of the material. The material rheology implemented is the Voellmy approach, because it acts very well evaluating the frictional and turbulent behavior. The FLATModel uses a GIS environment that facilitates the data analysis as the comparison between field and numerical data. The two events present two different characteristics, one is practically a one dimensional problem of 1400 m in length and the other has a more two dimensional behavior that forms a big fan.

  19. Western Pyrenees facing global change: comparison of the effects of climatic and anthropogenic change on water abstractions

    International Nuclear Information System (INIS)

    Terrasson, Isabelle; Chazot, Sebastien; Maton, Laure; Rinaudo, Jean-Daniel; Caballero, Yvan

    2014-01-01

    In the French Western Pyrenees, the trend to the decrease of low water flows that has been observed during the current years should be going on in the future. This may increase the hydric stress on aquatic ecosystems, and the competition among water uses and users for accessing water resources. The research project ANR-VULCAIN compared the impacts of climatic and socio-economic change on the hydro-systems of the French Western Pyrenees. Modeling and participative prospect analysis have been coupled to quantify the evolution of water abstractions under these two types of change. Socio-economic scenarios have been built together with local stakeholders during workshops (urbanism / land planning on the one hand and agriculture on the other hand). Their results have been quantified with the models developed so as to assess anthropogenic change impacts on domestic and agricultural abstractions. In parallel, the agricultural model has been fed with climatic scenarios so as to assess the impacts of climate change on agricultural water needs. In the created scenarios, the evolution of agricultural water needs under climate change have a bigger range than the evolution of abstractions for domestic water and agricultural needs under anthropic change, which are the same order of magnitude. To satisfy this evolution, there are some rooms to maneuver: make distribution modalities more efficient, optimize the management of storage capacity, or use substitution resources. This paper presents the approach that has been followed, and some of the main results. (authors)

  20. The broad-spectrum caspase inhibitor Boc-Asp-CMK induces cell death in human leukaemia cells.

    Science.gov (United States)

    Frydrych, Ivo; Mlejnek, Petr; Dolezel, Petr; Zoumpourlis, Vassilis; Krumpochova, Petra

    2008-08-01

    Synthetic caspase inhibitors and particularly broad-spectrum caspase inhibitors can prevent cells from death or at least slow down cell death process and abrogate some apoptotic hallmarks [Kitanaka, C., Kuchino, Y., 1999. Caspase-independent programmed cell death with necrotic morphology. Cell Death and Differentiation 6, 508-515]. However, not all synthetic caspase inhibitors diminish cell death. We have found that the broad-spectrum caspase inhibitor Boc-Asp-CMK induced cell death at micromolar concentrations in human leukaemia cells. Interestingly, low concentrations of Boc-Asp-CMK induced cell death with apoptotic hallmarks. Increasing concentrations of Boc-Asp-CMK led to necrotic cell death. The switch between apoptosis and necrosis seemed to depend upon the degree of inhibition of executioner caspases, including caspase-3/7 with Boc-Asp-CMK. Interestingly, caspase-3 processing was not inhibited even for the highest concentration of Boc-Asp-CMK used. We assume, that toxic properties of Boc-Asp-CMK can be attributed to the chloromethylketone residuum in its molecule, as its analogue Boc-Asp-FMK with fluoromethylketone residuum was more than 13 times less toxic. Our results further indicated that toxicity of Boc-Asp-CMK might arise from its interference with mitochondrial metabolism.

  1. Middle Jurassic shear zones at Cap de Creus (eastern Pyrenees, Spain) : a record of pre-drift extension of the Piemonte–Ligurian Ocean?

    NARCIS (Netherlands)

    Vissers, Reinoud L. M.; Van Hinsbergen, Douwe J. J.; Wilkinson, Camilla M.; Ganerød, Morgan

    The Cap de Creus peninsula in NE Spain consists of greenschist- to amphibolite-facies metasediments and granitoid bodies of the Variscan Axial Zone of the Pyrenees, overprinted in the north by anastomosed greenschist-facies shear zones. Current tectonic interpretations ascribe these shear zones to

  2. Mass transport-related stratal disruption within sedimentary melanges: Examples from the northern Apennines (Italy) and south-central Pyrenees (Spain)

    NARCIS (Netherlands)

    Ogata, Kei; Mutti, Emiliano; Pini, Gian Andrea; Tinterri, Roberto

    2012-01-01

    We report here mass transport-related disruption processes and their artifacts within sedimentary mélanges. The case studies include the early Oligocene wedge-top mass transport deposits in the northern Apennines (Italy) and the Eocene foredeep carbonate megabreccias from the south-central Pyrenees

  3. Mass transport-related stratal disruption within sedimentary m??langes: Examples from the northern Apennines (Italy) and south-central Pyrenees (Spain)

    NARCIS (Netherlands)

    Ogata, Kei; Mutti, Emiliano; Pini, Gian Andrea; Tinterri, Roberto

    2012-01-01

    We report here mass transport-related disruption processes and their artifacts within sedimentary m??langes. The case studies include the early Oligocene wedge-top mass transport deposits in the northern Apennines (Italy) and the Eocene foredeep carbonate megabreccias from the south-central Pyrenees

  4. Biostratigraphy, palaeoecology and palaeogeography of the mainly marine Ager Formation (Upper Paleocene — Lower Eocene) in the Tremp Basin, Central-South Pyrenees, Spain

    NARCIS (Netherlands)

    Gaemers, Pieter A.M.

    1978-01-01

    During the greater part of the Palaeogene the Tremp Basin was an area which underwent rapid subsidence as compared with the axial zone of the Pyrenees to the north, and the Ebro Massif to the south. As a result the sea occupied this area for a long time and deposition of the Ager Formation took

  5. Topology of AspT, the aspartate:alanine antiporter of Tetragenococcus halophilus, determined by site-directed fluorescence labeling.

    Science.gov (United States)

    Nanatani, Kei; Fujiki, Takashi; Kanou, Kazuhiko; Takeda-Shitaka, Mayuko; Umeyama, Hideaki; Ye, Liwen; Wang, Xicheng; Nakajima, Tasuku; Uchida, Takafumi; Maloney, Peter C; Abe, Keietsu

    2007-10-01

    The gram-positive lactic acid bacterium Tetragenococcus halophilus catalyzes the decarboxylation of L-aspartate (Asp) with release of L-alanine (Ala) and CO(2). The decarboxylation reaction consists of two steps: electrogenic exchange of Asp for Ala catalyzed by an aspartate:alanine antiporter (AspT) and intracellular decarboxylation of the transported Asp catalyzed by an L-aspartate-beta-decarboxylase (AspD). AspT belongs to the newly classified aspartate:alanine exchanger family (transporter classification no. 2.A.81) of transporters. In this study, we were interested in the relationship between the structure and function of AspT and thus analyzed the topology by means of the substituted-cysteine accessibility method using the impermeant, fluorescent, thiol-specific probe Oregon Green 488 maleimide (OGM) and the impermeant, nonfluorescent, thiol-specific probe [2-(trimethylammonium)ethyl]methanethiosulfonate bromide. We generated 23 single-cysteine variants from a six-histidine-tagged cysteineless AspT template. A cysteine position was assigned an external location if the corresponding single-cysteine variant reacted with OGM added to intact cells, and a position was assigned an internal location if OGM labeling required cell lysis. The topology analyses revealed that AspT has a unique topology; the protein has 10 transmembrane helices (TMs), a large hydrophilic cytoplasmic loop (about 180 amino acids) between TM5 and TM6, N and C termini that face the periplasm, and a positively charged residue (arginine 76) within TM3. Moreover, the three-dimensional structure constructed by means of the full automatic modeling system indicates that the large hydrophilic cytoplasmic loop of AspT possesses a TrkA_C domain and a TrkA_C-like domain and that the three-dimensional structures of these domains are similar to each other even though their amino acid sequences show low similarity.

  6. The mountainous space as a commodity: the Pyrenees at the age of globalization

    Directory of Open Access Journals (Sweden)

    Ismael Vaccaro

    2009-12-01

    Full Text Available In these pages we reflect on the process of transformation of the Catalan Pyrenees from a peripheral and economically decaying area to a first-rate tourism center. Real estate speculation has replaced depopulation. The economic and social value of the territory has dramatically shifted as it has gone from supporting low profitability ranching activities to become the space where resorts and parks are built. The paper analyzes the process of commodification and patrimonialization that have reintegrated the area into the national and international leisure markets paying special attention to the role of conservation practices, ski resorts and second residences in such a process. Finally, we attempt to identify the similarities and differences that characterize the two emergent tourism models: mass tourism versus small scale ecotourism.Au fil de ces pages, nous réfléchissons au processus de transformation des Pyrénées catalanes de zone périphérique et en déclin économique en centre touristique de qualité. La spéculation immobilière a remplacé le dépeuplement. La valeur économique et sociale du territoire a radicalement changé, la promotion des activités d’élevage peu rentables ayant cédé la place aux stations touristiques et aux parcs. Cet article analyse le processus de marchandisation et de patrimonialisation qui a réintégré la région sur les marchés de loisirs nationaux et internationaux en prêtant une attention particulière au rôle des pratiques de conservation, des stations de ski et des résidences secondaires. Enfin, nous tentons d’identifier les similitudes et les différences qui caractérisent les deux modèles touristiques émergents : le tourisme de masse contre l’écotourisme à l’échelle locale.

  7. Positive effects of millennial grazing on soils in the western French Pyrenees

    Science.gov (United States)

    Leigh, David; Gragson, Ted; Coughlan, Michael

    2015-04-01

    Many hillslopes of the western French Pyrenees have been grazed for thousands of years following the introduction of sheep about 7500 years ago, yet little is known about the long-term effects of pastoral activities on soil properties and pedogenic processes in this humid-temperate mountain range. In the 13 square kilometer Basque commune of Larrau we compare the status of soils under old pastures to those under old-growth forests at elevations ranging from 1000 to 1600 masl. Four separate tracts of side-by-side pairs of pasture and old-growth forest were sampled to discriminate differences in physical and chemical soil properties. Five paired soil profile samples were taken from each vegetation type on each tract so that all factors of soil formation, except vegetation type, were similar for each pair. Animal trails were excluded from sampling. We also developed radiocarbon chronologies of sedimentation rates from colluvial deposits at four other pasture sites to evaluate possible differences in the magnitude of soil erosion and sedimentation before and after conversion to pastures during the Holocene. Results indicate pastured A horizons are about three times as thick as forested soils, have significantly lower soil bulk densities, and much finer and stronger structural development of soil aggregates. These traits favor much greater infiltration and water holding capacities of the pastured soils. Thus, very significant pedogenic reorganization occurred in the pastures that can be viewed as improvements in soil quality. Inorganic nutrients in the pastured soils have significantly lower concentrations than in forested soils, except that amorphous silica is more abundant within pastured soils presumably due to greater phytolith production. The amount of nutrient depletion does not appear to be a limiting factor for grass growth and biomass production. Sedimentation chronologies indicate that erosion and sedimentation rates slightly increased following the earliest

  8. Isotopic composition of dissolved inorganic nitrogen in high mountain lakes: variation with altitude in the Pyrenees

    Science.gov (United States)

    Bartrons, M.; Camarero, L.; Catalan, J.

    2010-05-01

    Nitrogen deposition in remote areas has increased, but the effect on ecosystems is still poorly understood. For aquatic systems, knowledge of the main processes driving the observed variation is limited, as is knowledge of how changes in nitrogen supply affect lake biogeochemical and food web processes. Differences in dissolved inorganic nitrogen (DIN) between lakes cannot be understood without considering catchment characteristics. In mountains, catchment features (e.g., thermal conditions, land cover) vary considerably with elevation. The isotopic composition of nitrogen (δ15N) is increasingly used to study aquatic ecosystem dynamics. Here we explore the variability of δ15N in DIN in high mountain lakes and show that environmental conditions that change with altitude can affect the isotopic ratio. We measured ammonium and nitrate δ15N values in atmospheric deposition, epilimnetic water, deep chlorophyll maximum water (DCMW) and sediment pore water (SPW) from eight mountain lakes in the Pyrenees, both above and below the treeline. Lakes showed relatively uniform δ15N-NH4+ values in SPW (2.2±1.6‰), with no variation corresponding to catchment or lake characteristics. We suggest that organic matter diagenesis under similar sediment conditions is responsible for the low variation between the lakes. In the water column, the range of δ15N values was larger for ammonium (-9.4‰ to 7.4‰) than for nitrate (-11.4‰ to -3.4‰), as a result of higher variation both between and within lakes (epilimnetic vs. DCM water). For both compounds part of the difference correlated with altitude or catchment features (e.g., scree proportion). Based on concentration, chemical and isotopic tendencies, we suggest that patterns arise from the distinct relative contributions of two types of water flow paths to the lakes: one from snowpack melting, with little soil interaction; and another highly influenced by soil conditions. The snow-type flow path contributes low DIN

  9. Radiometric dating of brittle fault rocks; illite polytype age analysis and application to the Spanish Pyrenees.

    Science.gov (United States)

    van der Pluijm, B. A.; Haines, S. H.

    2008-12-01

    A variety of approaches have been available to indirectly date the timing of deformation and motion on faults, but few approaches for direct, radiometric dating of shallow crustal fault rocks were available until recently. The growing recognition of clay neomineralization at low temperatures in many fault rocks, particularly the 1Md illite polytype, allows the successful application of Ar dating to these K-bearing phases. In this presentation we will discuss our recent illite age analysis approach (sampling, treatments, analytical methods), and present new results from fault dating along the Spanish Pyrenean orogenic front as an example. X-ray quantification of polytype ratios in three or more size fractions is used to define a mixing line between (1Md illite) authigenic and (2M illite) detrital end-member phases that constrain the fault age and host rock provenance/cooling age for each fault. The common problem of recoil in clays is addressed by encapsulating samples before irradiation. Nine fault gouge ages in the south-central and south-eastern Pyrenees support several contractional pulses in the Pyrenean orogen: 1) Late Cretaceous thrusting (Boixols), 2) Latest Paleocene-Early Eocene deformation (Nogueres Zone and Freser antiformal stack), 3) Middle-Late Eocene deformation (Ripoll syncline, Vallfogona, Gavernie, Abocador and L'Escala thrusts), and 4) Middle Oligocene thrusting in the central portion of the Axial Zone (Llavorsi-Senet). The late Paleocene-Early Eocene and Middle-Late Eocene events may or may not be one single phase, due to slightly overlapping error estimates. The outboard thrusts give Hercynian ages for the detrital component of the fault rock, while the inboard thrusts, which juxtapose metamorphic units, give Cretaceous ages for the non-authigenic component, reflecting the cooling age of the adjacent wallrocks. Based on our latest work, the illite polytype dating method complements previously developed illite-smectite dating (van der Pluijm et

  10. Smart Valley Infrastructure.

    Science.gov (United States)

    Maule, R. William

    1994-01-01

    Discusses prototype information infrastructure projects in northern California's Silicon Valley. The strategies of the public and private telecommunications carriers vying for backbone services and industries developing end-user infrastructure technologies via office networks, set-top box networks, Internet multimedia, and "smart homes"…

  11. Breathing Valley Fever

    Centers for Disease Control (CDC) Podcasts

    2014-02-04

    Dr. Duc Vugia, chief of the Infectious Diseases Branch in the California Department of Public Health, discusses Valley Fever.  Created: 2/4/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 2/5/2014.

  12. Pro-domain removal in ASP-2 and the cleavage of the amyloid precursor are influenced by pH

    Directory of Open Access Journals (Sweden)

    Austen Brian

    2002-08-01

    Full Text Available Abstract Background One of the signatures of Alzheimer's disease is the accumulation of aggregated amyloid protein, Aβ, in the brain. Aβ arises from cleavage of the Amyloid Precursor protein by β and γ secretases, which present attractive candidates for therapeutic targeting. Two β-secretase candidates, ASP-1 and ASP-2, were identified as aspartic proteases, both of which cleave the amyloid precursor at the β-site. These are produced as immature transmembrane proteins containing a pro-segment. Results ASP-2 expressed in HEK293-cells cleaved the Swedish mutant amyloid precursor at different β-sites at different pHs in vitro. Recent reports show that furin cleaves the pro-peptide of ASP-2, whereas ASP-1 undergoes auto-catalysis. We show that purified recombinant ASP-2 cleaves its own pro-peptide at ph 5 but not pH 8.5 as seen by mass spectrometry, electrophoresis and N-terminal sequencing. Conclusion We suggest that ASP-2 processing as well as activity are influenced by pH, and hence the cellular localisation of the protein may have profound effects on the production of Aβ. These factors should be taken into consideration in the design of potential inhibitors for these enzymes.

  13. The AgI/II family adhesin AspA is required for respiratory infection by Streptococcus pyogenes.

    Directory of Open Access Journals (Sweden)

    Linda Franklin

    Full Text Available Streptococcus pyogenes (GAS is a human pathogen that causes pharyngitis and invasive diseases such as toxic shock syndrome and sepsis. The upper respiratory tract is the primary reservoir from which GAS can infect new hosts and cause disease. The factors involved in colonisation are incompletely known however. Previous evidence in oral streptococci has shown that the AgI/II family proteins are involved. We hypothesized that the AspA member of this family might be involved in GAS colonization. We describe a novel mouse model of GAS colonization of the nasopharynx and lower respiratory tract to elucidate these interactions. We used two clinical M serotypes expressing AspA, and their aspA gene deletant isogenic mutants in experiments using adherence assays to respiratory epithelium, macrophage phagocytosis and neutrophil killing assays and in vivo models of respiratory tract colonisation and infection. We demonstrated the requirement for AspA in colonization of the respiratory tract. AspA mutants were cleared from the respiratory tract and were deficient in adherence to epithelial cells, and susceptible to phagocytosis. Expression of AspA in the surrogate host Lactococcus lactis protected bacteria from phagocytosis. Our results suggest that AspA has an essential role in respiratory infection, and may function as a novel anti-phagocytic factor.

  14. Prominent Intracytoplasmic Crystals in Alveolar Soft Part Sarcoma (ASPS): An Aid in Cytological Diagnosis.

    Science.gov (United States)

    Mannan, Rahul; Bhasin, Tejinder Singh; Kaur, Parampreet; Manjari, Mridu; Gill, Karamjit Singh

    2014-02-01

    Alveolar soft part sarcoma (ASPS) is a rare neoplasm of unknown histogenesis with poor prognosis. Due to the epithelioid appearance of the neoplastic cells, ASPS may resemble many neoplastic conditions, such as metastatic epithelial cell tumours with clear cell change, metastatic renal cell carcinoma, granular cell tumour, epithelioid sarcoma, malignant melanoma and even paragangliomas. Presence of abundant, rod like crystals in the cytoplasm of tumour cells is an important finding characteristic of this tumour, which helps in differentiating it from the other entities. The case study highlights the importance of correlating cytological features that help in reaching the diagnosis such as the background, cell morphology and presence of characteristic rod shaped crystals as immunohistochemical studies are often non-conclusive. The case also is unique as it demonstrates presence of intra-cytoplamic crystals in such abundance.

  15. GLU298ASP and 4G/5G Polymorphisms and the Risk of Ischemic Stroke in Young Individuals.

    Science.gov (United States)

    Esparza-García, Juan Carlos; Santiago-Germán, David; Guadalupe Valades-Mejía, María; Hernández-Juárez, Jesus; Aguilar-Sosa, Eberth; Leaños-Miranda, Alfredo; Alvarado-Moreno, Antonio; Majluf-Cruz, Abraham; Isordia-Salas, Irma

    2015-09-01

    Polymorphisms in the endothelial nitric oxide synthase (eNOS) and in the plasminogen activator inhibitor -1 (PAI-1) genes have been implicated in stroke pathogenesis but results are still controversial. The aim of this study was to examine the possible contribution of Glu298Asp in the eNOS and 4G/5G in the PAI-1polymorphisms with ischemic stroke in a young Mexican population. In a case-control study, conducted between January 2006 and June 2010, 204 patients ≤45 years of age with ischemic stroke and 204 controls matched by age and gender, were recruited. The Glu298Asp and 4G/5G polymorphisms were determined in all participants by polymerase chain reaction-restriction fragment length polymorphism. There was a significant difference in the Glu298Asp genotype distribution (P=0.001) and allele frequency between the two groups (P=0.001). The 4G/5G genotype distribution (P=0.40) and the allele frequency was similar between groups; (P=0.13). There were independent factors for ischemic stroke: Asp carriage (GluAsp+AspAsp) (P=0.02); smoking (P=0.01); hypertension (P=0.03), and familial history of atherothrombotic disease (P=0.04). The Asp allele from the Gu298Asp gene represents an independent risk factor for ischemic stroke in a young Mexican population. In contrast, the 4G/5G was not associated with an increased risk for this disease in the same group of patients, as previously has been demonstrated in other populations.

  16. Characterization and evolutionary implications of the triad Asp-Xxx-Glu in group II phosphopantetheinyl transferases.

    Science.gov (United States)

    Wang, Yue-Yue; Li, Yu-Dong; Liu, Jian-Bo; Ran, Xin-Xin; Guo, Yuan-Yang; Ren, Ni-Ni; Chen, Xin; Jiang, Hui; Li, Yong-Quan

    2014-01-01

    Phosphopantetheinyl transferases (PPTases), which play an essential role in both primary and secondary metabolism, are magnesium binding enzymes. In this study, we characterized the magnesium binding residues of all known group II PPTases by biochemical and evolutionary analysis. Our results suggested that group II PPTases could be classified into two subgroups, two-magnesium-binding-residue-PPTases containing the triad Asp-Xxx-Glu and three-magnesium-binding-residue-PPTases containing the triad Asp-Glu-Glu. Mutations of two three-magnesium-binding-residue-PPTases and one two-magnesium-binding-residue-PPTase indicate that the first and the third residues in the triads are essential to activities; the second residues in the triads are non-essential. Although variations of the second residues in the triad Asp-Xxx-Glu exist throughout the whole phylogenetic tree, the second residues are conserved in animals, plants, algae, and most prokaryotes, respectively. Evolutionary analysis suggests that: the animal group II PPTases may originate from one common ancestor; the plant two-magnesium-binding-residue-PPTases may originate from one common ancestor; the plant three-magnesium-binding-residue-PPTases may derive from horizontal gene transfer from prokaryotes.

  17. Systemic toxoplasmosis and Gram-negative sepsis in a southern chamois (Rupicapra pyrenaica) from the Pyrenees in northeast Spain.

    Science.gov (United States)

    Marco, Ignasi; Velarde, Roser; López-Olvera, Jorge R; Cabezón, Oscar; Pumarola, Martí; Lavín, Santiago

    2009-03-01

    A 6-year-old, male southern chamois (Rupicapra pyrenaica) had an absence of flight response and was captured by hand in the Catalan Pyrenees in northeast Spain. On clinical examination, the animal was in good body condition, and only atrophy of the right eye was observed. Blood samples were collected and hematologic analysis performed, but no alterations were observed. The animal was sent to a Wildlife Rescue Centre, where it developed chronic wasting and died after 32 days in captivity. At necropsy, the animal was cachectic and had edematous, mottled lungs. Histopathologic examination revealed systemic toxoplasmosis and acute Gram-negative septicemia. The protozoan organisms were identified as Toxoplasma gondii based on immunohistochemistry. An indirect fluorescent antibody test was performed, and the animal was positive with an antibody titer of 150.

  18. High resolution imaging of lithospheric structures beneath the Pyrenees by full waveform inversion of shortperiod teleseismic P waves

    Science.gov (United States)

    Wang, Yi; Chevrot, Sébastien; Komatitsch, Dimitri; Monteiller, Vadim; Durochat, Clément

    2016-04-01

    Thanks to the deployment of permanent and temporary broadband arrays, coverage and data quality have dramatically improved in the last decade, especially for regional-scale studies. In addition, owing to the progress of high-performance resources and numerical simulation techniques, waveform inversion approaches nowadays become a viable alternative to classical asymptotic ray based tomographic approaches. Exploiting full waveforms in seismic tomography requires an efficient and precise method to solve the elastic wave equation in 3D inhomogeneous media. Since resolution of waveform inversion is limited by the seismic wavelength as well as the wavefield sampling density, it is crucial to exploit short-period teleseismic waves recorded by dense regional arrays. However, modeling the propagation of short-period body waves in heterogeneous media is still very challenging, even on the largest modern supercomputers. For this reason, we have developed a hybrid method that couples a global wave propagation method in a 1D Earth to a 3D spectral-element method in a regional domain. This hybrid method restricts the costly 3D computations to inside the regional domain, which dramatically decreases the computational cost, allows us to compute teleseismic wavefields down to 1s period, thus accounting for the complexities that affect the propagation of seismic waves in the regional domain. We present the first application of this new waveform inversion approach to broadband data coming from two dense transects deployed during the PYROPE experiment across the Pyrenees mountains. We obtain the first high-resolution lithospheric section of compressional and shear velocities across an orogenic belt. The tomographic model provides clear evidence for the under-thrusting of the thinned Iberian crust beneath the European plate and for the important role of rift-inherited mantle structures during the formation of the Pyrenees.

  19. Retrospective study of pestivirus infection in Pyrenean chamois (Rupicapra pyrenaica) and other ungulates in the Pyrenees (NE Spain).

    Science.gov (United States)

    Marco, Ignasi; Cabezón, Oscar; Rosell, Rosa; Fernández-Sirera, Laura; Allepuz, Alberto; Lavín, Santiago

    2011-04-21

    In 2001 a new Pestivirus (Family Flaviviridae) was associated with an outbreak of a previously unreported disease in Pyrenean chamois (Rupicapra pyrenaica) in the Pyrenees (NE Spain). Molecular characterization assigned this virus to the Border Disease Virus (BDV) cluster, BDV-4 genotype. A retrospective study was performed in archived sera and spleen of 74 Pyrenean chamois and in archived sera of 28 mouflon (Ovis ammon), 56 red deer (Cervus elaphus), 43 roe deer (Capreolus capreolus) and 29 fallow deer (Dama dama) from the Pyrenees between the years 1990 and 2000. Thirty six of 74 (48.6%) sera of Pyrenean chamois, one of mouflon and one of red deer were positive by an ELISA antibody test. Comparative virus neutralization tests were performed on 26 seropositive chamois, one mouflon and one red deer, using five pestivirus strains. An ELISA antigen test was performed on 37 seronegative chamois and yielded positive results in one chamois and inconclusive result in two. RT-PCR and virus isolation performed on spleen samples from these three animals gave positive results in the positive and one inconclusive animal. Sequence analysis in the 5' unstranslated region revealed that they were grouped into the BDV-4 genotype. Virological and serological data of the present study indicate that BDV infection has been present in the chamois population since at least 1990, 11 years before the first outbreak of disease. Therefore, the emergence of the disease in 2001 is apparently due to other factors rather than the introduction of a new virus in the chamois population. Copyright © 2010 Elsevier B.V. All rights reserved.

  20. Bed load size distribution and flow conditions in a high mountain catchment of Central Pyrenees

    Directory of Open Access Journals (Sweden)

    Martínez-Castroviejo, Ricardo

    1990-06-01

    Full Text Available The bed load size distribution caused by different types of flow are compared in a high mountain catchment located in the upper Gallego river basin (Central Spanish’ Pyrenees. Three kinds of hydrologic events could be defined: those triggered by heavy autumn rainfalls, those originated by isolated summer rainstorms and those promoted by snowmelting. Each one is characterized by a peculiar bed load size distribution. Thus, it could be demonstrated that the coarser fractions, above 30 mm in diameter, are up to six times more abundant, in percentage of total weight, in transports caused by heavy rainfalls than in the material collected after snowmelt flows. In its turn, bed load mobilized by snowmelt flows is mainly composed by medium and fine gravel, from 2 to 8 mm. These may amount up to 60 % of total weight of bed load. The reasons for these so different size distributions are discussed.

    [es] En una cuenca de alta montaña localizada en el alto valle del río Gallego (Pirineo central se comparan las distribuciones por tamaños de los acarreos movilizados por diferentes tipos de caudal. Tres tipos de eventos hidrológicos han podido ser caracterizados: los ocasionados por intensas lluvias de otoño, los originados por tormentas estivales aisladas y los producidos por la fusión de la nieve acumulada durante el invierno. Se concluye que cada uno de ellos lleva asociada una distribución por tamaños típica de la carga de fondo. Así, se ha comprobado que las fracciones más gruesas consideradas -superiores a los 30 mm de diámetro- son hasta seis veces más abundantes -en porcentaje sobre el peso total- en las exportaciones causadas por lluvias de gran intensidad que en las generadas por caudales de fusión. A su vez, las descargas ocasionadas por la fusión arrastran principalmente gravas de calibre medio y fino -entre 2y8 mm- que llegan a suponer el 60 % en peso del volumen movilizado. Este artículo discute las razones que provocan

  1. Identifying erosive periods by using RUSLE factors in mountain fields of the Central Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    M. López-Vicente

    2008-03-01

    Full Text Available The Mediterranean environment is characterized by strong temporal variations in rainfall volume and intensity, soil moisture and vegetation cover along the year. These factors play a key role on soil erosion. The aim of this work is to identify different erosive periods in function of the temporal changes in rainfall and runoff characteristics (erosivity, maximum intensity and number of erosive events, soil properties (soil erodibility in relation to freeze-thaw processes and soil moisture content and current tillage practices in a set of agricultural fields in a mountainous area of the Central Pyrenees in NE Spain. To this purpose the rainfall and runoff erosivity (R, the soil erodibility (K and the cover-management (C factors of the empirical RUSLE soil loss model were used. The R, K and C factors were calculated at monthly scale. The first erosive period extends from July to October and presents the highest values of erosivity (87.8 MJ mm ha−1 h−1, maximum rainfall intensity (22.3 mm h−1 and monthly soil erosion (0.25 Mg ha−1 month−1 with the minimum values of duration of erosive storms, freeze-thaw cycles, soil moisture content and soil erodibility (0.007 Mg h MJ−1 mm−1. This period includes the harvesting and the plowing tillage practices. The second erosive period has a duration of two months, from May to June, and presents the lowest total and monthly soil losses (0.10 Mg ha−1 month−1 that correspond to the maximum protection of the soil by the crop-cover ($C$ factor = 0.05 due to the maximum stage of the growing season and intermediate values of rainfall and runoff erosivity, maximum rainfall intensity and soil erodibility. The third erosive period extends from November to April and has the minimum values of rainfall erosivity (17.5 MJ mm ha−1 h−1 and

  2. Numerical modelling comparison of slow landslides: the Portalet case study (Central Pyrenees-Spain)

    Science.gov (United States)

    Fernandez-Merodo, Jose Antonio; Garcia-Davalillo, Juan Carlos; Herrera, Gerardo

    2013-04-01

    Slow-moving landslides are a wide-spread type of active mass movement that cause severe damages to infrastructures and may be a precursor of sudden catastrophic slope failures. In this context, modelling slow-moving landslide behaviour is an important task in order to quantify and reduce the risk associated to this geological process. In practice, landslide occurrence and stability conditions are evaluated for a given scenario through a stability factor based on limit equilibrium analysis. This "static" approximation is hardly applied when boundary conditions are time dependent. Apart from earthquake studies, time dependent analysis is required when: (i) hydrological conditions change as in the case of rainfall; (ii) resistant parameters are reduced as in the case of strain softening or weathering processes and (iii) creep behaviour is taken into account. Different numerical models can be applied to reproduce the kinematic behaviour of large slow landslides. This paper compares four different models: i) a direct correlation with measured rainfall, ii) a simple 1D infinite slope viscoplastic model [1], iii) a 2D elasto-plastic finite element model [2] and iv) a 2D visco-plastic finite element model [3]. These models, ordered by increasing level of complexity, are compared by applying them to the Portalet case study. The Portalet landslide (Central Spanish Pyrenees) is an active paleo-landslide that has been "reactivated" by the construction of a parking area at the toe of the slope in 2004. This landslide is still active despite the corrective measures carried out to stabilize it. The measurements obtained with different monitoring techniques (ground based SAR, advanced DInSAR processing of satellite SAR images, DGPS and inclinometers) indicate that the hillside is still moving today following two patterns. The first one corresponds to a slow continuous motion of constant speed of about 100 mm/year, the second one corresponds to accelerations of the moving mass when

  3. Soil removed by voles of the genus Pitymys in the Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    Borghi, C. E.

    1990-12-01

    Full Text Available The erosiogenic activity of Pyrenean mountain voles is studied following the measures taken in an experimental plot in the Western Pyrenees. An easy model for estimating the volume and weight of soil carried to the surface by voles is presented and used to quantify this amount in natural conditions. Fossorial Pyrenean rodents seem to dislodge well over 6Tm/ha.yr of soil on the colonized areas above the timberline. The four stages (new, recent, old, and vegetated of the evolution of soil heaps are discussed. Finally, an attempt is made to evaluate the rate of horizontal sediment transport due to the direct action of voles, with a maximum result of 17 cm3/cm.yr, quite comparable to pure geoclimatic rates.

    [es] Se estudia la actividad de movimiento del suelo de los roedores pirenaicos del género Pitymys, a partir de los datos obtenidos en una parcela experimental situada en los Pirineos Occidentales. Se presenta un modelo sencillo para estimar la cantidad de tierra removida a partir de medidas que pueden tomarse fácilmente en el campo, y se emplea dicho modelo para evaluar esta magnitud en condiciones naturales. Al parecer, los roedores subterráneos pueden sacar al exterior más de 6 Tm de tierra por hectárea y año en las zonas epiforestales que colonizan. También se discute la evolución del suelo removido y sus condiciones para la erosión por escorrentía. Finalmente se intenta evaluar la tasa de transporte horizontal del sedimento debida a los animales, que resulta ser de hasta 17 cm3 por cm y año, un valor claramente comparable con los debidos a agentes geoclimáticos.
    [fr] On a étudié l'activité fouisseuse des campagnols pyrénéens du genre Pitymys, d'après les données recueillies dans une enclosure expérimentale située dans les Pyrénées de l'Ouest. On présente un modèle simple permettant d'estimer la quantité de sol mue par les campagnols a partir de mésurements qu

  4. Radionuclides and stable elements in the sediments of the Yesa Reservoir, Central Spanish Pyrenees

    Energy Technology Data Exchange (ETDEWEB)

    Navas, Ana; Gaspar, Leticia; Palazon, Leticia [Estacion Experimental Aula Dei (EEAD- CSIC), Zaragoza (Spain). Dept. of Soil and Water; Valero-Garces, Blas [Instituto Pirenaico de Ecologia (IPE-CSIC), Zaragoza (Spain)

    2011-09-15

    Purpose: The sediments accumulated in the Yesa Reservoir (Central Spanish Pyrenees) have greatly decreased its water storage capacity and are a major threat to the sustainability of water resources in the region. This study examines the contents of radionuclides and stable elements in the reservoir sediments and relates their variations with the sediment composition and local sedimentary dynamics, particularly flood frequency and intensity, which are responsible for changes in the main supply and distribution of radionuclides in the basin. Materials and methods: The sedimentary sequence accumulated in the Yesa Reservoir (471 Hm{sup 3}), which supplies water to ca. 1,000,000 people and for irrigation, was examined in two 4-m long sediment cores (Y1, Y2) and one profile (Y3) retrieved at its central part. In the sediments, radionuclide activities of {sup 238}U, {sup 226}Ra, {sup 232}Th, {sup 40}K, {sup 210}Pb and {sup 137}Cs were measured using a hyperpure Ge coaxial detector. The stable elements Mg, Ca, Sr, Ba, Cr, Cu, Mn, Fe, Al, Zn, Ni, Co, Pb, Li, K and Na were analysed by ICP-OES. Complementary analyses to characterize the sediments included: XRD in the profile, grain size distribution by laser equipment and the contents of organic matter, carbonates and the residual fraction by loss on ignition. Results and discussion: The variation in radionuclide activities is associated with grain size and sediment composition. The activity levels (becquerels per kilogram) ranged between 20 and 43 for{sup 238}U, 14 and 40 for {sup 226}Ra, 7 and 56 for {sup 210}Pb, 19 and 46 for Th{sup 232}, 1 and 48 for {sup 137}Cs and 185 and 610 for {sup 40}K. Enriched activity levels are associated with clayey and silty layers, and depleted levels with sandy layers. The levels of radionuclides and trace elements were significantly lower in the cores than in the profile because of its higher silicate content and the influence of inflow of spring mineral-rich waters. The correlations among

  5. Isotopic composition of dissolved inorganic nitrogen in high mountain lakes: variation with altitude in the Pyrenees

    Directory of Open Access Journals (Sweden)

    M. Bartrons

    2010-05-01

    Full Text Available Nitrogen deposition in remote areas has increased, but the effect on ecosystems is still poorly understood. For aquatic systems, knowledge of the main processes driving the observed variation is limited, as is knowledge of how changes in nitrogen supply affect lake biogeochemical and food web processes. Differences in dissolved inorganic nitrogen (DIN between lakes cannot be understood without considering catchment characteristics. In mountains, catchment features (e.g., thermal conditions, land cover vary considerably with elevation. The isotopic composition of nitrogen (δ15N is increasingly used to study aquatic ecosystem dynamics. Here we explore the variability of δ15N in DIN in high mountain lakes and show that environmental conditions that change with altitude can affect the isotopic ratio.

    We measured ammonium and nitrate δ15N values in atmospheric deposition, epilimnetic water, deep chlorophyll maximum water (DCMW and sediment pore water (SPW from eight mountain lakes in the Pyrenees, both above and below the treeline. Lakes showed relatively uniform δ15N-NH4+ values in SPW (2.2±1.6‰, with no variation corresponding to catchment or lake characteristics. We suggest that organic matter diagenesis under similar sediment conditions is responsible for the low variation between the lakes.

    In the water column, the range of δ15N values was larger for ammonium (−9.4‰ to 7.4‰ than for nitrate (−11.4‰ to −3.4‰, as a result of higher variation both between and within lakes (epilimnetic vs. DCM water. For both compounds part of the difference correlated with altitude or catchment features (e.g., scree proportion. Based on concentration, chemical and isotopic tendencies, we suggest that patterns arise from the distinct relative contributions of two types of water flow paths to the lakes: one from snowpack melting, with little soil

  6. Rift Valley fever vaccines

    OpenAIRE

    Ikegami, Tetsuro; Makino, Shinji

    2009-01-01

    Rift Valley fever virus (RVFV), which belongs to the genus Phlebovirus, family Bunyaviridae, is a negative-stranded RNA virus carrying a tripartite RNA genome. RVFV is transmitted by mosquitoes and causes large outbreaks among ruminants and humans in Africa and the Arabian Peninsula. Human patients develop an acute febrile illness, followed by a fatal hemorrhagic fever, encephalitis or ocular diseases, whereas ruminants experience abortions during outbreak. Effective vaccination of both human...

  7. Fundamental Roles of the Golgi-Associated Toxoplasma Aspartyl Protease, ASP5, at the Host-Parasite Interface

    Czech Academy of Sciences Publication Activity Database

    Hammoudi, P.-M.; Jacot, D.; Mueller, C.; Di Cristina, M.; Dogga, S.K.; Marq, J.-B.; Romano, J.; Tosetti, N.; Dubrot, J.; Emre, Y.; Lunghi, M.; Coppens, I.; Yamamoto, M.; Sojka, Daniel; Pino, P.; Soldati-Favre, D.

    2015-01-01

    Roč. 11, č. 10 (2015), e1005211 E-ISSN 1553-7374 Institutional support: RVO:60077344 Keywords : Toxoplasma gondii * Plasmodium proteins * ASP5 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 7.003, year: 2015

  8. Enhanced humoral response to influenza vaccine in aged mice with a novel adjuvant, rOv-ASP-1.

    Science.gov (United States)

    Jiang, Jiu; Fisher, Erin M; Concannon, Mark; Lustigman, Sara; Shen, Hao; Murasko, Donna M

    2016-02-10

    Immunization is the best way to prevent seasonal epidemics and pandemics of influenza. There are two kinds of influenza vaccines available in the United States: an inactivated vaccine (TIV) and an attenuated vaccine; however, only TIV is approved for immunization of the elderly population. While the aged population has the highest rate of influenza vaccination, the protective efficacy is low as evidenced by elderly individuals having the highest mortality associated with influenza. Recently, we reported that an adjuvant derived from the helminth parasite Onchocerca volvulus, named O. volvulus activation-associated secreted protein-1 (Ov-ASP-1), can significantly enhance the protective efficacy of an inactivated vaccine (TIV) in young adult mice. In the current study, we examined whether this recombinant Ov-ASP-1 (rOv-ASP-1) can enhance the efficacy of TIV in aged mice as well. While primary immunization with TIV alone produced only a low level of influenza-specific antibodies (total IgG, IgG1, and IgG2c) in aged mice, the antibody levels were significantly increased after immunization with TIV+rOv-ASP-1. More importantly, the level of the total IgG in aged mice administered TIV+rOv-ASP-1 was comparable to that of young adult mice immunized with TIV alone. Co-administration of rOv-ASP-1 induced a low level of cross-reactive antibody and enhanced the protective efficacy of TIV in aged mice, reflected by significantly increased survival after challenge with a heterologous influenza virus. rOv-ASP-1 was also superior to the conventional adjuvant alum in inducing specific IgG after TIV immunization in aged mice, and in conferring protection after challenge. These results demonstrate that rOv-ASP-1 may serve as a potential adjuvant for influenza vaccine to improve the efficacy of protection in the elderly. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. PROTOTIPE PEMESANAN BAHAN PUSTAKA MELALUI WEB MENGGUNAKAN ACTIVE SERVER PAGE (ASP

    Directory of Open Access Journals (Sweden)

    Djoni Haryadi Setiabudi

    2002-01-01

    Full Text Available Electronic commerce is one of the components in the internet that growing fast in the world. In this research, it is developed the prototype for library service that offers library collection ordering especially books and articles through World Wide Web. In order to get an interaction between seller and buyer, there is an urgency to develop a dynamic web, which needs the technology and software. One of the programming languages is called Active Server Pages (ASP and it is combining with database system to store data. The other component as an interface between application and database is ActiveX Data Objects (ADO. ASP has an advantage in the scripting method and it is easy to make the configuration with database. This application consists of two major parts those are administrator and user. This prototype has the facilities for editing, searching and looking through ordering information online. Users can also do downloading process for searching and ordering articles. Paying method in this e-commerce system is quite essential because in Indonesia not everybody has a credit card. As a solution to this situation, this prototype has a form for user who does not have credit card. If the bill has been paid, he can do the transaction online. In this case, one of the ASP advantages will be used. This is called "session" when data in process would not be lost as long as the user still in that "session". This will be used in user area and admin area where the users and the admin can do various processes. Abstract in Bahasa Indonesia : Electronic commerce adalah satu bagian dari internet yang berkembang pesat di dunia saat ini. Pada penelitian ini dibuat suatu prototipe program aplikasi untuk pengembangan jasa layanan perpustakaan khususnya pemesanan artikel dan buku melalui World Wide Web. Untuk membangun aplikasi berbasis web diperlukan teknologi dan software yang mendukung pembuatan situs web dinamis sehingga ada interaksi antara pembeli dan penjual

  10. Pro single page application development using Backbone.js and ASP.NET

    CERN Document Server

    Fink, Gil

    2014-01-01

    One of the most important and exciting trends in web development in recent years is the move towards single page applications, or SPAs. Instead of clicking through hyperlinks and waiting for each page to load, the user loads a site once and all the interactivity is handled fluidly by a rich JavaScript front end. If you come from a background in ASP.NET development, you'll be used to handling most interactions on the server side. Pro Single Page Application Development will guide you through your transition to this powerful new application type.The book starts in Part I by laying the groundwork

  11. Razvoj in analiza zmogljivosti rešitev na osnovi ogrodja ASP .NET Core

    OpenAIRE

    Jerenko, Tomi

    2017-01-01

    V diplomskem delu smo spoznali principe in dobre prakse oblačnega računalništva ter lastnosti ogrodij za razvoj spletnih aplikacij. Kot glavno temo diplomskega dela smo predstavili kratko zgodovino ogrodja ASP .NET Core, nato pa še principe delovanja funkcionalnosti in primere uporabe. V praktičnem delu diplomskega dela smo prikazali potek namestitve aplikacije, zgrajene z ogrodjem, na strežnike Apache, Nginx in IIS ter za vsakega izvajali obremenitvene teste, katerih rezultate smo na koncu p...

  12. Application of ASP Technology to Realize the Online Administrative License of the earthquake in Hunan Province

    Science.gov (United States)

    Tang, Hongliang; Kang, Chengxu; Tian, Youping

    2018-01-01

    Realizing the online handling of administrative approval of earthquakes is an important measure to improve work efficiency and facilitate people’s convenience. Based on the analysis of the characteristics and processes of the administrative licensing in the earthquake industry, this paper proposes an online processing model based on ASP technology and an online processing system based on B/S architecture. This paper presents the design and implementation methods. The application of the system shows that the system is simple in design and full in function, and can be used on mobile platforms such as computers and mobile phones, and has good practicability and forward-lookingness.

  13. The California Valley grassland

    Science.gov (United States)

    Keeley, J.E.; Schoenherr, Allan A.

    1990-01-01

    Grasslands are distributed throughout California from Oregon to Baja California Norte and from the coast to the desert (Brown 1982) (Figure 1). This review will focus on the dominant formation in cismontane California, a community referred to as Valley Grassland (Munz 1959). Today, Valley Grassland is dominated by non-native annual grasses in genera such as Avena (wild oat), Bromus (brome grass), and Hordeum (barley), and is often referred to as the California annual grassland. On localized sites, native perennial bunchgrasses such as Stipa pultra (purple needle grass) may dominate and such sites are interpreted to be remnants of the pristine valley grassland. In northwestern California a floristically distinct formation of the Valley Grassland, known as Coast Prairie (Munz 1959) or Northern Coastal Grassland (Holland and Keil 1989) is recognized. The dominant grasses include many native perennial bunchgrasses in genera such as Agrostis, Calamagrostis, Danthonia, Deschampsia, Festuca, Koeleria and Poa (Heady et al. 1977). Non-native annuals do not dominate, but on some sites non-native perennials like Anthoxanthum odoratum may colonize the native grassland (Foin and Hektner 1986). Elevationally, California's grasslands extend from sea level to at leas 1500 m. The upper boundary is vague because montane grassland formations are commonly referred to as meadows; a community which Munz (1959) does not recognize. Holland and Keil (1989) describe the montane meadow as an azonal community; that is, a community restricted not so much to a particular climatic zone but rather controlled by substrate characteristics. They consider poor soil-drainage an over-riding factor in the development of montane meadows and, in contrast to grasslands, meadows often remain green through the summer drought. Floristically, meadows are composed of graminoids; Cyperaceae, Juncaceae, and rhizomatous grasses such as Agropyron (wheat grass). Some bunchgrasses, such as Muhlenbergia rigens, are

  14. Neolithic flint mines of Treviño (Basque-Cantabrian Basin, Western Pyrenees, Spain

    Directory of Open Access Journals (Sweden)

    Antonio Tarriño

    2014-09-01

    Full Text Available English:The prehistoric Treviño flint mine complex is located in the Sierra de Araico-Cucho (Berantevilla, Alava - Condado de Treviño, Burgos, inside the lacustrine-palustrine Cenozoic (Aquitanian, Miocene materials of the South-Pyrenean syncline of the Basque-Cantabrian Basin. It is a landscape unit constituted by a set of carbonated layers with abundant nodular and stratiform silicifications. The extraction mining works (often referred to as ‘tailing’ are usually identified as dumps or trenches, subtly visible and associated with archaeological materials.An archaeological excavation was carried out in one potential mining structure (dump or pit that was detected by LiDAR (Light Detection and Ranging in the mountain pass of “Pozarrate” near the villages of Grandival and Araico (Treviño, Burgos. In this work we present the results of the excavation of the last two years. The existence of a Neolithic mining dump (the tailings with a chronology ca. 5000 cal. BC was confirmed. The base rock level with nodular flint was reached and the impressions of the exploited nodules have been identified. As well, the extraction front which reaches about 4.0-5.0 metres in height was delimited. Thousands of lithic remains associated with the extraction and the initial processing (shaping of flint were collected, as along with mining tools. We have found and described three types of mining structures: trenches, linear dumps and crescent-shaped (or “half-moon-shaped” dumps.This site is one of the few prehistoric flint mines dated in the Iberian Peninsula. Recent investigations in the Cantabrian Mountains and Western Pyrenees indicate that the circulation and use of Treviño flint during Prehistory reached many Holocene and Pleistocene archaeological sites, located hundreds of kilometres away from the outcrops.Español:El complejo prehistórico minero de sílex de Treviño se sitúa en la Sierra de Araico-Cucho (Berantevilla, Alava - Condado de Trevi

  15. HABITAT AND HABITANTS IN THE CATALAN PYRENEES: DYNAMICS AND POLICIES FOR UNDER-POPULATED HIGH MOUNTAIN VILLAGES

    Directory of Open Access Journals (Sweden)

    Maria Rosa Bonet Casas

    2009-03-01

    Full Text Available This paper is the result of a research carried out in 2006 in a partnership between the Universitat de Barcelona and the Universitat Politècnica de Catalunya, which was aimed to define guidelines to recover the architectural and scenic values of villages in almost abandoned Pyrenean valleys in Catalunya. The research studied at different levels (geographical, urban and architectural the villages of several valleys in order to find out the factors more determinant to explain their degree of revitalization or decay. As a result of the research, the paper presents some conclusions on how to intervene on those pyrenean valleys: to work at the level of geographical units rather than with singular villages or individuals; to establish degrees of priority of intervention between the valleys in order to increase the efficiency of the actions; to ensure high quality infrastructure levels on the selected valleys; to fix strict limits to the construction of new vacation houses in high mountain villages in order to orient the real state demand to the rehabilitation of existing buildings; to create a program of subsidies to help the permanent population working on the primary sector to rehabilitate their dwellings; to promote the display of the natural and cultural richness of the valleys as a main factor for their revitalization; to support private and civic actions oriented to the recover of the valleys.

  16. Arg-Tyr-Asp (RYD) and Arg-Cys-Asp (RCD) motifs in dendroaspin promote selective inhibition of beta1 and beta3 integrins.

    Science.gov (United States)

    Wattam, B; Shang, D; Rahman, S; Egglezou, S; Scully, M; Kakkar, V; Lu, X

    2001-05-15

    Arg-Gly-Asp (RGD) is a unique minimal integrin-binding sequence that is found within several glycoprotein ligands. This sequence has also been found in snake-venom anti-platelet proteins, including the disintegrins and dendroaspin, a natural variant of short-chain neurotoxins isolated from the venom of Dendroaspis jamesonii. In the present study, the motifs RYD and RCD were introduced into the dendroaspin scaffold to replace RGD. Both motifs in dendroaspin caused inhibition of ADP-induced platelet aggregation with IC(50) values of 200 and 300 nM respectively, similar to that of the wild-type RGD motif (170 nM). In comparison with wild-type dendroaspin, both RYD- and RCD-containing dendroaspins were more selective in the inhibition of the adhesion of K562 cells to laminin rather than to fibrinogen and fibronectin, even though they were 10-30-fold less potent at inhibiting K562 cell (containing alpha(5)beta(1) integrin) adhesion to laminin compared with wild-type. Interestingly, the RYD motif produced a similar IC(50) value to the RGD motif at inhibiting A375-SM cell (beta(3) integrin) adhesion to collagen, whereas the RCD motif was approx. 2-6-fold less potent compared with either RGD or RYD. These findings show that the selectivity of dendroaspin binding to beta(1) and beta(3) integrins can be modulated by the introduction of alternative cell recognition sequences.

  17. Assessing Workshop Models for Informal Educators: ASP's "Astronomy from the Ground Up” Experiment.

    Science.gov (United States)

    Manning, Jim; Gurton, S.; Hurst Schmitt, A.; Pompea, S.; Glass, M.; Haley, K.

    2011-01-01

    The classic workshop model for science educators in the past has been largely in situ: you show up somewhere, meet your fellow workshoppers, get personal treatment from instructors over several intensive days of content delivery, illustrative activities, and practice in technique, and try to incorporate what you've learned once you get back. But in an age when everybody's digitally connected, and many can't afford to travel, can an "online” workshop be as effective? This was a key question in the Astronomical Society of the Pacific (ASP) NSF-funded project "Astronomy from the Ground Up,” designed to increase astronomy education capacity at small and medium-sized science and nature centers and museums around the U.S. Together with its institutional partners, the Association of Science and Technology Centers (ASTC) and the Institute for Learning Innovation (ILI), and a cadre of individual partners, the ASP conducted both on-site and online workshops and created an online community of practice to increase informal educator capacity to present astronomy to their audiences, and to evaluate the relative effectiveness of the on-site and online delivery schemes. The presenter(s) will share some initial results and findings of the project.

  18. Comparison of taurine, GABA, Glu, and Asp as scavengers of malondialdehyde in vitro and in vivo

    Science.gov (United States)

    Deng, Yan; Wang, Wei; Yu, Pingfeng; Xi, Zhijiang; Xu, Lijian; Li, Xiaolong; He, Nongyue

    2013-04-01

    The purpose of this study is to determine if amino acid neurotransmitters such as gamma-aminobutyric acid (GABA), taurine, glutamate (Glu), and aspartate (Asp) can scavenge activated carbonyl toxicants. In vitro, direct reaction between malondialdehyde (MDA) and amino acids was researched using different analytical methods. The results indicated that scavenging activated carbonyl function of taurine and GABA is very strong and that of Glu and Asp is very weak in pathophysiological situations. The results provided perspective into the reaction mechanism of taurine and GABA as targets of activated carbonyl such as MDA in protecting nerve terminals. In vivo, we studied the effect of taurine and GABA as antioxidants by detecting MDA concentration and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. It was shown that MDA concentration was decreased significantly, and the activities of SOD and GSH-Px were increased significantly in the cerebral cortex and hippocampus of acute epileptic state rats, after the administration of taurine and GABA. The results indicated that the peripherally administered taurine and GABA can scavenge free radicals and protect the tissue against activated carbonyl in vivo and in vitro.

  19. Project Overview: Cumulus Humilis Aerosol Processing Study (CHAPS): Proposed Summer 2007 ASP Field Campaign

    Energy Technology Data Exchange (ETDEWEB)

    Berkowitz, Carl M.; Berg, Larry K.; Ogren, J. A.; Hostetler, Chris A.; Ferrare, Richard

    2006-05-18

    This white paper presents the scientific motivation and preliminary logistical plans for a proposed ASP field campaign to be carried out in the summer of 2007. The primary objective of this campaign is to use the DOE Gulfstream-1 aircraft to make measurements characterizing the chemical, physical and optical properties of aerosols below, within and above large fields of fair weather cumulus and to use the NASA Langley Research Center’s High Spectral Resolution Lidar (HSRL) to make independent measurements of aerosol backscatter and extinction profiles in the vicinity of these fields. Separate from the science questions to be addressed by these observations will be information to add in the development of a parameterized cumulus scheme capable of including multiple cloud fields within a regional or global scale model. We will also be able to compare and contrast the cloud and aerosol properties within and outside the Oklahoma City plume to study aerosol processes within individual clouds. Preliminary discussions with the Cloud and Land Surface Interaction Campaign (CLASIC) science team have identified overlap between the science questions posed for the CLASIC Intensive Operation Period (IOP) and the proposed ASP campaign, suggesting collaboration would benefit both teams.

  20. Racial difference in Acylation Stimulating Protein (ASP correlates to triglyceride in non-obese and obese African American and Caucasian women

    Directory of Open Access Journals (Sweden)

    Cianflone Katherine

    2009-04-01

    Full Text Available Abstract Background Acylation Stimulating Protein (ASP has been shown to influence adipose tissue triglyceride (TG storage. The aim was to examine ethnic differences in ASP and leptin levels in relation to lipid profiles and postprandial changes amongst African American (AA and Caucasian American (CA women matched for BMI. Methods 129 women were recruited in total (age 21 – 73 y: 24 non-obese (BMI 2 CA, 27 obese (BMI ≥ 30 kg/m2 CA, 13 obese diabetic CA, 25 non-obese AA, 25 obese AA, and 15 obese diabetic AA. Cholesterol, HDL-C, LDL-C, apoB, glucose and insulin were measured at baseline. TG, non-esterified fatty acids, leptin, and ASP were measured at baseline and postprandially following a fat meal. Results ASP, leptin, insulin and TG were significantly increased in obese subjects within each race. However, AA women had significantly lower ASP and TG than CA women at all BMI. Obese and diabetic AA women had significantly lower apoB levels than CA women when compared to their respective counterparts. For AA women, fasting ASP was positively correlated with BMI, cholesterol, apoB, LDL-C and glucose. For CA women, fasting ASP was positively correlated with BMI, leptin, glucose and insulin. However, for any given BMI, ASP was significantly reduced in AA vs CA (p = 0.0004. Similarly, for any given leptin level or TG levels, ASP was significantly lower in AA women (p = 0.041 and p = 0.003, respectively. Conclusion CA women have higher baseline TG levels and an earlier TG peak that is accompanied with higher ASP levels suggesting increased ASP resistance, while AA women have lower baseline TG levels and a later TG peak at lower ASP levels suggesting increased ASP sensitivity. This may explain why AA women may have fewer metabolic complications, such as diabetes and CVD, when compared to their Caucasian counterparts at the same level of obesity.

  1. Investigating the role of polymer type and dead end pores’ distribution on oil recovery efficiency during ASP flooding

    Directory of Open Access Journals (Sweden)

    Mohammad Hossein Sedaghat

    2013-12-01

    Full Text Available Although alkaline-surfactant-polymer flooding is proved to be efficient for oil recovery from petroleum reservoirs, effects of existence of dead end pores on this process need more discussions. In this work, several ASP flooding tests constituted from 4 polymers, 1 surfactant and 1 alkaline were performed on micromodels designed in four various dead end pore distributions initially saturated with crude oil. The results showed that although using ASP solution constituted from hydrolyzed polymers at high molecular weights significantly increases oil recovery factor due to increasing apparent viscosity of the solution, using sulfonated polymers in ASP solution increases oil recovery much more because of their capability to increase viscosity even in saline solutions. In addition, it was concluded that the number of dead end pores as well as their distribution with respect to the flow direction are two main characteristics that identify the efficiency of brine and ASP floods in dead end porous media. Moreover, although in ASP flooding, since the viscosity is higher and the front is flatter, the role of the number of dead ends on the recovery efficiency is more identifiable than the role of dead ends’ distribution, in waterflooding, since the mobility ratio is not low enough, the role of dead end direction with respect to the flow direction plays a more significant role in recovery process. So, considering the efficient direction of injection is too important during waterflooding and chemical EOR especially in reservoirs that have a remarkable percentage of dead end pores in their geological structure.

  2. Structural and functional analysis of the ASM p.Ala359Asp mutant that causes acid sphingomyelinase deficiency.

    Science.gov (United States)

    Acuña, Mariana; Castro-Fernández, Víctor; Latorre, Mauricio; Castro, Juan; Schuchman, Edward H; Guixé, Victoria; González, Mauricio; Zanlungo, Silvana

    2016-10-21

    Niemann-Pick disease (NPD) type A and B are recessive hereditary disorders caused by deficiency in acid sphingomyelinase (ASM). The p.Ala359Asp mutation has been described in several patients but its functional and structural effects in the protein are unknown. In order to characterize this mutation, we modeled the three-dimensional ASM structure using the recent available crystal of the mammalian ASM as a template. We found that the p.Ala359Asp mutation is localized in the hydrophobic core and far from the sphingomyelin binding site. However, energy function calculations using statistical potentials indicate that the mutation causes a decrease in ASM stability. Therefore, we investigated the functional effect of the p.Ala359Asp mutation in ASM expression, secretion, localization and activity in human fibroblasts. We found a 3.8% residual ASM activity compared to the wild-type enzyme, without changes in the other parameters evaluated. These results support the hypothesis that the p.Ala359Asp mutation causes structural alterations in the hydrophobic environment where ASM is located, decreasing its enzymatic activity. A similar effect was observed in other previously described NPDB mutations located outside the active site of the enzyme. This work shows the first full size ASM mutant model describe at date, providing a complete analysis of the structural and functional effects of the p.Ala359Asp mutation over the stability and activity of the enzyme. Copyright © 2016 Elsevier Inc. All rights reserved.

  3. The influence of knowledge on the public policy making process: the case of renewable energies in Midi Pyrenees

    International Nuclear Information System (INIS)

    Behar, L.

    2012-01-01

    Even though energy policy makers were novices when wind power was introduced in the Midi Pyrenees region, the situation had changed when the photovoltaic was implemented. The new challenges policy makers experimented about the wind power and their opponents, the wood energy and the primacy of the wood's industrials, the photovoltaic and the preservation of the agricultural lands, fostered their learning and gradually lead them to change their perception of inherent issues. The coalitions forged between technical experts and policy makers unbind along the progressive empowerment of the formers. Shared between different forms of knowledge, however, the resources needed to implement renewable energies projects cannot be reduced to the technical dimension. Local knowledge and engineering knowledge increasingly compete with expert knowledge. This evolution corresponds to a policy arrangement change. Since the dynamic but also the elements that compose this political system change, a new reality gradually emerges. This whole process will be the main concern of this thesis. We will finally notice that there are some differences between the implementation of these renewable energies among the territories but also among the three forms of renewable energies we analyze. This means that although renewable energy policy are based on interactions between actors' resources, discourse and rules, they also emerge from the action of intermediates actors, and from the characteristic of each territories in which they are implemented. (author)

  4. Tree-ring based assessment of rockfall frequency on talus slopes at Solà d'Andorra, Eastern Pyrenees

    Science.gov (United States)

    Moya, J.; Corominas, J.; Pérez Arcas, J.; Baeza, C.

    2010-06-01

    The determination of the frequency of rockfall events continues to challenge quantitative hazard assessments in most mountain areas. Dendrogeomorphological analysis was used to assess rockfall frequency on talus slopes at Solà d'Andorra (Eastern Pyrenees, Andorra). Rockfall events were dated at two sites: one at the outlet of a chute, and the other below a rock wall. The impact wounds visible on the tree surface were analyzed with a seasonal temporal resolution. At each site, trees were sampled in three forest strips 15 to 30 m wide located at different heights on the talus. All the trees with visible injuries in the strips were sampled. Rockfall frequency cannot be assessed by a simple analysis of time series of tree damage. A satisfactory assessment requires a prior interpretation of the location of the damaged trees in relation to a number of rockfall events. At Solà d'Andorra, the rockfall chronology was reconstructed for the last 25 years by dating visible wounds. For older periods, the tree wound record of rockfalls is incomplete owing to progressive closure of wounds. Wounds exceeding 40 years in age had already disappeared from the tree surface in the study area. Our results show a clear reduction of the rockfall frequency down the talus, a noticeable lateral change in frequency, and an influence of source morphology (chute or wall) on rockfall activity on the talus.

  5. A method for upscaling soil parameters for use in a dynamic modelling assessment of water quality in the Pyrenees.

    Science.gov (United States)

    Camarero, Lluís; Garcia-Pausas, Jordi; Huguet, Carme

    2009-02-15

    Dynamic modelling of hydrochemistry is a valuable tool to study and predict the recovery of surface waters from acidification, and to assess the effects of confounding factors (such as delayed soil response and changing climate) that cause hysteresis during reversal from acidification. The availability of soil data is often a limitation for the regional application of dynamic models. Here we present a method to upscale site-specific soil properties to a regional scale in order to circumvent that problem. The method proposed for upscaling relied on multiple regression models between soil properties and a suite of environmental variables used as predictors. Soil measurements were made during a field survey in 13 catchments in the Pyrenees (NW Spain). The environmental variables were derived from mapped or remotely sensed topographic, lithological, land-cover, and climatic information. Regression models were then used to model soil parameters, which were supplied as input for the biogeochemical model MAGIC (Model for Acidification of Groundwater In Catchments) in order to reconstruct the history of acidification in Pyrenean lakes and forecast the recovery under a scenario of reduced acid deposition. The resulting simulations were then compared with model runs using field measurements as input parameters. These comparisons showed that regional averages for the key water and soil chemistry variables were suitably reproduced when using the modelled parameters. Simulations of water chemistry at the catchment scale also showed good results, whereas simulated soil parameters reflected uncertainty in the initial modelled estimates.

  6. On the reliability of AMS in ilmenite-type granites: an insight from the Marimanha pluton, central Pyrenees

    Science.gov (United States)

    Oliva-Urcia, B.; Casas, A. M.; Ramón, M. J.; Leiss, B.; Mariani, E.; Román-Berdiel, T.

    2012-04-01

    The anisotropy of magnetic susceptibility (AMS) at room temperature has been used for decades to obtain the petrofabric orientation in granites as a kinematic marker to establish models explaining the emplacement of plutons. To assess the significance of AMS in terms of mineral orientation, we have performed a multidisciplinary study at five sites of an ilmenite-type pluton (Marimanha, central Pyrenees) with significant facies changes. To test the reliability of AMS measurements at room temperature, the following methods were applied: low temperature AMS; image analyses and X-ray texture goniometry (XTG) of biotites; and electron backscatter diffraction (EBSD) to obtain c-axes directions of quartz. The total (para-, ferro- and dia-)magnetic fabric analysed by AMS is compared with the paramagnetic fabric (low-T AMS), mica orientation (with image analyses and XTG) and the diamagnetic fabric (EBSD). Results indicate that weakly oriented paramagnetic minerals can give well-defined magnetic fabrics (AMS at room and low temperatures). Furthermore, the AMS ellipsoid is the result of composite biotite fabrics resulting from both orientation and spatial distribution of crystals, as demonstrated by 2-D mathematical models presented in this study. AMS is the most effective technique for quickly measuring composite fabrics. In addition, the advantage of using AMS analyses is twofold: (1) it is a fast way of analysing standard samples that can give clues for subsequent image/mineral orientation analysis and (2) it is a volume-related method that gives a picture of the rock fabric as a whole.

  7. Saw-tooth structures and curved veins related to folds in the south-central Pyrenees (Spain)

    Science.gov (United States)

    Bastida, F.; Aller, J.; Lisle, R. J.; Bobillo-Ares, N. C.; Menéndez, C. O.

    2012-01-01

    Two generation of folds ( F1 and F2) and associated structures, developed in the Eocene turbidites of the south-central Pyrenees, are analyzed in this paper. F1 folds are close, have sub-horizontal axes and southwards vergence. They have an associated cleavage S1. Competent layers were folded by layer-parallel shortening, tangential longitudinal strain, some possible flexural flow and an obliquely superimposed homogeneous strain due mainly to simple shear. Flexural slip is also an important mechanism in the whole multilayer. F2 folds are gentle and scarce; they fold the S1 cleavage. Among the structures associated with F1 folds, there are sets of veins with curved form in the competent layers. The displacement of each vein gave rise usually to a step in the layer boundary, so that a set of veins produces a structure that is named "saw-tooth structure". The veins initiated as small faults that made flexural slip difficult and gave rise to a concentration of stress on the steps, leading to an opening of the fractures and a propagation of them along a curved path, as suggested by a simple mechanical model. This propagation agrees with finite element models developed by other authors.

  8. Valley development on Hawaiian volcanoes

    International Nuclear Information System (INIS)

    Baker, V.R.; Gulick, V.C.

    1987-01-01

    Work in progress on Hawaiian drainage evolution indicates an important potential for understanding drainage development on Mars. Similar to Mars, the Hawaiian valleys were initiated by surface runoff, subsequently enlarged by groundwater sapping, and eventually stabilized as aquifers were depleted. Quantitative geomorphic measurements were used to evaluate the following factors in Hawaiian drainage evolution: climate, stream processes, and time. In comparing regions of similar climate, drainage density shows a general increase with the age of the volcani island. With age and climate held constant, sapping dominated valleys, in contrast to runoff-dominated valleys, display the following: lower drainage densities, higher ratios of valley floor width to valley height, and more positive profile concavities. Studies of stream junction angles indicate increasing junction angles with time on the drier leeward sides of the major islands. The quantitative geomorphic studies and earlier field work yielded important insights for Martian geomorphology. The importance of ash mantling in controlling infiltration on Hawaii also seems to apply to Mars. The Hawaiian valley also have implications for the valley networks of Martian heavily cratered terrains

  9. Substrate Specificity of the Aspartate:Alanine Antiporter (AspT) of Tetragenococcus halophilus in Reconstituted Liposomes*

    Science.gov (United States)

    Sasahara, Ayako; Nanatani, Kei; Enomoto, Masaru; Kuwahara, Shigefumi; Abe, Keietsu

    2011-01-01

    The aspartate:alanine antiporter (AspT) of the lactic acid bacterium Tetragenococcus halophilus is a member of the aspartate:alanine exchanger (AAEx) transporter family. T. halophilus AspT catalyzes the electrogenic exchange of l-aspartate1− with l-alanine0. Although physiological functions of AspT were well studied, l-aspartate1−:l-alanine0 antiport mechanisms are still unsolved. Here we report that the binding sites of l-aspartate and l-alanine are independently present in AspT by means of the kinetic studies. We purified His6-tagged T. halophilus AspT and characterized its kinetic properties when reconstituted in liposomes (Km = 0.35 ± 0.03 mm for l-aspartate, Km = 0.098 ± 0 mm for d-aspartate, Km = 26 ± 2 mm for l-alanine, Km = 3.3 ± 0.2 mm for d-alanine). Competitive inhibition by various amino acids of l-aspartate or l-alanine in self-exchange reactions revealed that l-cysteine selectively inhibited l-aspartate self-exchange but only weakly inhibited l-alanine self-exchange. Additionally, l-serine selectively inhibited l-alanine self-exchange but barely inhibited l-aspartate self-exchange. The aspartate analogs l-cysteine sulfinic acid, l-cysteic acid, and d-cysteic acid competitively and strongly inhibited l-aspartate self-exchange compared with l-alanine self-exchange. Taken together, these kinetic data suggest that the putative binding sites of l-aspartate and l-alanine are independently located in the substrate translocation pathway of AspT. PMID:21719707

  10. Dark Valley in Newton Crater

    Science.gov (United States)

    2003-01-01

    MGS MOC Release No. MOC2-418, 11 July 2003This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) high resolution image shows part of a dark-floored valley system in northern Newton Crater. The valley might have been originally formed by liquid water; the dark material is probably sand that has blown into the valley in more recent times. The picture was acquired earlier this week on July 6, 2003, and is located near 39.2oS, 157.9oW. The picture covers an area 2.3 km (1.4 mi) across; sunlight illuminates the scene from the upper left.

  11. Evaluation of Ga-DOTA-(D-Asp)n as bone imaging agents: D-aspartic acid peptides as carriers to bone

    OpenAIRE

    Ogawa, Kazuma; Ishizaki, Atsushi; Takai, Kenichiro; Kitamura, Yoji; Makino, Akira; Kozaka, Takashi; Kiyono, Yasushi; Shiba, Kazuhiro; Odani, Akira

    2017-01-01

    67Ga-DOTA-(L-Asp)11 and 67Ga-DOTA-(L-Asp)14, which have been developed as bone imaging agents, showed a high accumulation in bone and a rapid blood clearance in mice. However, peptides composed of D-amino acids are more stable in vivo than those composed of their L-equivalents. In this study, 67Ga-DOTA-(D-Asp)n (n = 2, 5, 8, 11, or 14) were synthesized using the Fmoc-based solid-phase methodology and evaluated. In hydroxyapatite binding assay, binding of 67Ga-DOTA-(D-Asp)n tended to increase ...

  12. 27 CFR 9.41 - Lancaster Valley.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Lancaster Valley. 9.41... Lancaster Valley. (a) Name. The name of the viticultural area described in this section is “Lancaster Valley.” (b) Approved maps. The appropriate maps for determining the boundaries of the Lancaster Valley...

  13. Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite

    Directory of Open Access Journals (Sweden)

    Alessandro Pistone

    2017-02-01

    Full Text Available Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped hydroxyapatite (HA may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C, or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethyl­thiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

  14. Asp3Gly polymorphism affects fatty acid-binding protein 3 intracellular stability and subcellular localization.

    Science.gov (United States)

    Kusudo, Tatsuya; Hashida, Yasuhiko; Ando, Fujiko; Shimokata, Hiroshi; Yamashita, Hitoshi

    2015-08-19

    Fatty acid-binding proteins (FABP) play a crucial role in intracellular fatty acid transportation and metabolism. In this study, we investigate the effects of the FABP3 Asp3Gly (D3G) polymorphism on protein structure and function. Although the mutation did not alter protein secondary structure or the ability to bind 1-anilinonaphthalene-8-sulfonic acid and palmitate, the intracellular stability of the D3G mutant was significantly decreased. Immunocytochemical analysis reveals that the mutation alters FABP3 subcellular localization. Our results suggest that the D3G polymorphism may impact energy metabolism and physiological functions. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  15. Formation of quantum wires and dots on InP(001) by As/P exchange

    International Nuclear Information System (INIS)

    Yang, Haeyeon; Ballet, P.; Salamo, G. J.

    2001-01-01

    We report on the use of in situ scanning tunneling microscopy to study As/P exchange on InP(001) surfaces by molecular beam epitaxy. Results demonstrate that the exchange process can be controlled to selectively produce either quantum wires or quantum dots. 15 nm wide self-assembled nanowires are observed, and they are elongated along the dimer row direction of the InP(001)-2x4 surface with a length of over 1 μm and flat top 2x4 surfaces. In addition, when the nanowires are annealed with no arsenic overpressure, the surface reconstruction transforms from 2x4 to 4x2 and the nanowires transform into dots with a rectangular base and flat top. [copyright] 2001 American Institute of Physics

  16. From hyper-extended rifts to orogens: the example of the Mauléon rift basin in the Western Pyrenees (SW France)

    Science.gov (United States)

    Masini, E.; Manatschal, G.; Tugend, J.

    2011-12-01

    An integral part of plate tectonic theory is that the fate of rifted margins is to be accreted into mountain belts. Thus, rift-related inheritance is an essential parameter controlling the evolution and architecture of collisional orogens. Although this link is well accepted, rift inheritance is often ignored. The Pyrenees, located along the Iberian and European plate boundary, can be considered as one of the best places to study the reactivation of former rift structures. In this orogen the Late Cretaceous and Tertiary convergence overprints a Late Jurassic to Lower Cretaceous complex intracontinental rift system related to the opening of the North Atlantic. During the rifting, several strongly subsiding basins developed in the axis of the Pyrenees showing evidence of extreme crustal extension and even locale mantle exhumation to the seafloor. Although the exact age and kinematics of rifting is still debated, these structures have an important impact in the subsequent orogenic overprint. In our presentation we discuss the example of the Mauléon basin, which escaped from the most pervasive deformations because of its specific location at the interface between the western termination of the chain and the Bay of Biscay oceanic realm. Detailed mapping combined with seismic reflection, gravity data and industry wells enabled to determine the 3D rift architecture of the Mauléon basin. Two major diachronous detachment systems can be mapped and followed through space. The Southern Mauléon Detachment (SMD) develops first, starts to thin the crust and floors the Southern Mauléon sub-Basin (SMB). The second, the Northern Mauléon Detachment (SMD) is younger and controls the final crustal thinning and mantle exhumation to the north. Both constitute the whole Mauléon basin. Like at the scale of the overall Pyrenees, the reactivation of the Mauléon Basin increases progressively from west to east, which enables to document the progressive reactivation of an aborted hyper

  17. The Drentsche Aa valley system

    International Nuclear Information System (INIS)

    Gans, W. de.

    1981-01-01

    This thesis is composed of five papers concerned with Late Quaternary geology and geomorphology of the Aa valley system. The correlation and chronostratigraphic position of the layers have been established by radiocarbon dating. (Auth.)

  18. Application of the SWAT model to an endorheic watershed in the Central Spanish Pre-Pyrenees: Methodological approach and preliminary results

    Science.gov (United States)

    Gaspar, Leticia; White, Sue; Navas, Ana; López-Vicente, Manuel; Palazón, Leticia

    2013-04-01

    Modelling runoff and sediment transport at watershed scale are key tools to predict hydrological and sediment processes, identify soil sediment sources and estimate sediment yield, with the purpose of better managing soil and water resources. This study aims to apply the SWAT model in an endorheic watershed in the Central Spanish Pre-Pyrenees, where there have been a number of previous field-based studies on sediment sources and transfers. The Soil and Water Assessment Tool (SWAT) is a process based semi-distributed watershed scale hydrologic model, which can provide a high level of spatial detail by allowing the watershed to be divided into sub-basins. This study addresses the challenge of applying the SWAT model to an endorheic watershed that drains to a central lake, without external output, and without a network of permanent rivers. In this case it has been shown that the SWAT model does not correctly reproduce the stream network when using automatic watershed delineation, even with a high resolution Digital Elevation Model (5 x 5 metres). For this purpose, different approaches needed to be considered, such as i) user-defined watersheds and streams, ii) burning in a stream network or iii) modelling each sub-watershed separately. The objective of this study was to develop a new methodological approach for correctly simulating the main hydrological processes in an endorheic and complex karst watershed of the Spanish Pre-Pyrenees. The Estanque de Arriba Lake watershed (74 ha) is an endorheic system located in the Spanish Central Pre-Pyrenees. This watershed holds a small and permanent lake of fresh water (1.7 ha) and is a Site of Community Importance (European NATURA 2000 network). The study area is characterized by an abrupt topography with altitude range between 679 and 862 m and an average slope gradient of 24 %. Steep slopes (> 24 %) occupy the northern part of the watershed, whereas gentle slopes (

  19. Warming or cooling in the Pragian? Sedimentary record and petrophysical logs across the Lochkovian-Pragian boundary in the Spanish Central Pyrenees

    Czech Academy of Sciences Publication Activity Database

    Slavík, Ladislav; Valenzuela-Ríos, J. I.; Hladil, Jindřich; Chadimová, Leona; Liao, J.-Ch.; Hušková, Aneta; Calvo, H.; Hrstka, Tomáš

    2016-01-01

    Roč. 449, May (2016), s. 300-321 ISSN 0031-0182 R&D Projects: GA ČR GA14-18183S Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M100131201 Institutional support: RVO:67985831 Keywords : climate change * correlation * Early Devonian * gamma-ray spectrometry * magnetic susceptibility * Pyrenees Subject RIV: DB - Geology ; Mineralogy Impact factor: 2.578, year: 2016

  20. La Guerra Civil en Aspe. Las dos caras de la represión. El caso de la familia Calpena

    OpenAIRE

    García Gandía, José Ramón

    2016-01-01

    Análisis de la represión en la retaguardia republicana y de la posguerra en el pueblo de Aspe, a raíz de los procesos judiciales que tuvieron lugar en la relación al linchamiento de la familia Calpena en julio de 1937.

  1. Right Ventricular Adaptation Is Associated with the Glu298Asp Variant of the NOS3 Gene in Elite Athletes.

    Directory of Open Access Journals (Sweden)

    Zsolt Szelid

    Full Text Available Nitric oxide (NO, an important endogenous pulmonary vasodilator is synthetized by the endothelial NO synthase (NOS3. Reduced NO bioavailability and thus the Glu298Asp polymorphism of NOS3 may enhance right ventricular (RV afterload and hypertrophic remodeling and influence athletic performance. To test this hypothesis world class level athletes (water polo players, kayakers, canoeists, rowers, swimmers, n = 126 with a VO2 maximum greater than 50ml/kg/min were compared with non-athletic volunteers (n = 155. Cardiopulmonary exercise tests and cardiac magnetic resonance imaging (cMRI were performed to determine structural or functional changes. Genotype distribution of the NOS3 Glu298Asp polymorphism was not affected by gender or physical performance. Cardiac MRI showed increased stroke volume with eccentric hypertrophy in all athletes regardless of their genotype. However, the Asp allelic variant carriers had increased RV mass index (32±6g versus 27±6g, p<0.01 and larger RV stroke volume index (71±10ml versus 64±10ml, p<0.01 than athletes with a Glu/Glu genotype. Genotype was not significantly associated with athletic performance. In the non-athletic group no genotype related differences were detected. The association between the NOS3 Glu298Asp polymorphism and RV structure and dimension in elite athletes emphasizes the importance of NOS3 gene function and NO bioavailability in sport related cardiac adaptation.

  2. Right Ventricular Adaptation Is Associated with the Glu298Asp Variant of the NOS3 Gene in Elite Athletes.

    Science.gov (United States)

    Szelid, Zsolt; Lux, Árpád; Kolossváry, Márton; Tóth, Attila; Vágó, Hajnalka; Lendvai, Zsuzsanna; Kiss, Loretta; Maurovich-Horvat, Pál; Bagyura, Zsolt; Merkely, Béla

    2015-01-01

    Nitric oxide (NO), an important endogenous pulmonary vasodilator is synthetized by the endothelial NO synthase (NOS3). Reduced NO bioavailability and thus the Glu298Asp polymorphism of NOS3 may enhance right ventricular (RV) afterload and hypertrophic remodeling and influence athletic performance. To test this hypothesis world class level athletes (water polo players, kayakers, canoeists, rowers, swimmers, n = 126) with a VO2 maximum greater than 50ml/kg/min were compared with non-athletic volunteers (n = 155). Cardiopulmonary exercise tests and cardiac magnetic resonance imaging (cMRI) were performed to determine structural or functional changes. Genotype distribution of the NOS3 Glu298Asp polymorphism was not affected by gender or physical performance. Cardiac MRI showed increased stroke volume with eccentric hypertrophy in all athletes regardless of their genotype. However, the Asp allelic variant carriers had increased RV mass index (32±6g versus 27±6g, pathletes with a Glu/Glu genotype. Genotype was not significantly associated with athletic performance. In the non-athletic group no genotype related differences were detected. The association between the NOS3 Glu298Asp polymorphism and RV structure and dimension in elite athletes emphasizes the importance of NOS3 gene function and NO bioavailability in sport related cardiac adaptation.

  3. Stability metabolism and transport of D-Asp(OBzl)Ala -a model prodrug with affinity for the oligopeptide transporter

    DEFF Research Database (Denmark)

    Steffansen, Bente; Lepist, E-I; Frøkjær, Sven

    1999-01-01

    in different gastrointestinal media arising from rats and humans, as well as in human plasma. The second major aim of the study was to evaluate our previous study in Caco-2 cell culture, by determining the effective intestinal permeability (Peff) of D-Asp(OBzl)-Ala in situ using the single-pass rat perfusion...

  4. Study of Water-Oil Emulsion Breaking by Stabilized Solution Consisting of Anionic Surface Acting Agent - Soda Ash - Polymer (ASP)

    Science.gov (United States)

    Kulichkov, S. V.; Avtomonov, E. G.; Andreeva, L. V.; Solomennik, S. F.; Nikitina, A. V.

    2018-01-01

    The paper provides a laboratory research of breaking natural water-oil emulsions: - by non-stabilized ASP; by stabilized ASP; by mixture of stabilized and non-stabilized ASP in different proportions and production of refinery water of the required quality with the use of IronGuard 2495 as flocculant. Oil-in-water emulsion is stable. Classic methods are not suitable for residual water treatment: sediment gravity flow; filtration; centrifuge test. Microemulsion formed after ASP application has low boundary tension and high pH. It contributes to transfer of oil phase into a water one, forming oil-in-water emulsion. Alkaline condition has adverse effect on demulsifying ability of agents, flocculation and boundary tension. For breaking of water-oil emulsion at EBU before the interchanger water or water-oil emulsion from the wells that were not APS-treated in ratio of 1:9 shall be delivered. Residual water after EBU must be prepared in water tanks by dilution in great volume.

  5. Activation of an Asp-124→Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagine

    NARCIS (Netherlands)

    Pries, Frens; Kingma, Jacob; JANSSEN, Dick B

    1995-01-01

    Haloalkane dehalogenase hydrolyses various 1-halon-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed

  6. Proper receptor signalling in a mutant catfish gonadotropin-releasing hormone receptor lacking the highly conserved Asp(90) residue

    NARCIS (Netherlands)

    Blomenrohr, M.; Kuhne, R.; Hund, E.; Leurs, R.; Bogerd, J.; ter Laak, T.L.

    2001-01-01

    The negatively charged side chain of an Asp residue in transmembrane domain 2 is likely to play an important role in receptor signalling since it is highly conserved in the whole family of G protein-coupled receptors, except in mammalian gonadotropin-releasing hormone (GnRH) receptors. In this paper

  7. Middle-to-late Holocene palaeoenvironmental reconstruction from the A294 ice-cave record (Central Pyrenees, northern Spain)

    Science.gov (United States)

    Sancho, Carlos; Belmonte, Ánchel; Bartolomé, Miguel; Moreno, Ana; Leunda, María; López-Martínez, Jerónimo

    2018-02-01

    Perennial ice deposits in caves represent unique, but underexplored, terrestrial sequences that potentially contain outstanding palaeoclimatic records. Here, we present a pioneer palaeoenvironmental study of an ice deposit preserved in a small sag-type cave (A294) in the Central Pyrenees (northern Iberian Peninsula). The 9.25-m-thick sequence, which is dated from 6100 ± 107 to 1888 ± 64 cal BP, represents the oldest known firn ice record worldwide. The stratigraphy (detrital layers, unconformities, and cross stratification), plant macrofossils, and isotopic signature (similarity between the ice linear distribution, δ2H = 7.83δ18O + 8.4, and the Global Meteoric Water Line) of the ice point to the diagenesis of snow introduced to the cave by winter snowstorms. Four phases of rapid ice accumulation (6100-5515, 4945-4250, 3810-3155, and 2450-1890 cal BP) are related to wetter and colder winters. Comparison of the isotopic composition (δ18O and deuterium excess) of the ice with other paleoclimate records show that both source effects and the North Atlantic Oscillation (NAO) mechanism exert a dominant influence on the ice cave record. The NAO signal may be a combination of source effects and rainfall amount. Three intervals with low ice accumulation occurred between the phases of rapid accumulation and were related to drier, and possibly warmer, winters. These centennial-scale episodes appear to be in-phase with regional arid events, as established from high altitude lacustrine records and can be correlated to global Rapid Climate Change events. The current warming trend has dramatically decreased the volume of the ice deposit in cave A294.

  8. Assessing the effect of soil use changes on soil moisture regimes in mountain regions. (Catalan Pre-Pyrenees NE Spain)

    International Nuclear Information System (INIS)

    Loaiza Usuga, Juan Carlos; Jarauta Bragulat, Eusebio; Porta Casanellas, Jaume; Poch Claret, Rosa Maria

    2010-01-01

    Soil moisture regimes under different land uses were observed and modeled in a representative forest basin in the Catalonian Pre-Pyrenees, more specifically in the Ribera Salada catchment (222.5 km2). The vegetation cover in the catchment consists of pasture, tillage and forest. A number of representative plots for each of these land cover types were intensely monitored during the study period. The annual precipitation fluctuates between 516 and 753 mm, while the soil moisture content oscillates between 14 and 26% in the middle and low lying areas of the basin, and between 21 and 48% in shady zones near the river bed, and in the higher parts of the basin. Soil moisture and rainfall are controlled firstly by altitude, with the existence of two climatic types in the basin (sub-Mediterranean and sub-alpine), and further, by land use. Two models were applied to the estimated water moisture regimes: the Jarauta Simulation Newhall model (JSM) and the Newhall simulation model (NSM) were found to be able to predict the soil moisture regimes in the basin in the different combinations of local abiotic and biotic factors. The JSM results are more precise than the results obtained using another frequently used method, more specifically the Newhall Simulation Model (NSM), which has been developed to simulate soil moisture regimes. NSM was found to overestimate wet soil moisture regimes. The results show the importance of the moisture control section size and Available Water Capacity (AWC) of the profile, in the moisture section control state and variability. The mountain soils are dominated by rustic and occasionally xeric regimes. Land use changes leading to an increase in forest areas would imply drier soil conditions and therefore drier soil water regimes. These effects are most evident in degraded shallow and stony soils with low AWC.

  9. Streamflow response and sediment yield after farmland abandonment: results from a small experimental catchment in the Central Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    Serrano-Muela, M. P.

    2010-12-01

    Full Text Available A small catchment affected by agricultural practices in the past, then progressively abandoned and naturally re-vegetated was monitored in the central Spanish Pyrenees. The results obtained over a 9-year period revealed the complexity of its hydrological and geomorphological behaviour. Several runoff generation processes can occur at the same time and in different parts of the catchment, depending on the water reserves conditions and rainfall characteristics. Sediment response is mainly controlled by the spatial and temporal dynamics of water and sediment contributing areas within the catchment. The sediment output illustrates the complexity of the geomorphic response of mountain environments modified by past farmland activities.

    Se presenta una síntesis de la investigación realizada en una pequeña cuenca experimental afectada por una intensa actividad agraria en el pasado y sujeta a un proceso de revegetación natural en la actualidad en el Pirineo Central. Los principales resultados obtenidos demuestran su complejo comportamiento hidrogeomorfológico. El análisis de la información hidrológica sugiere que diferentes procesos dominantes de generación de escorrentía pueden ocurrir de manera simultánea en diferentes áreas de la cuenca, en función del estado de humedad de la misma y de las características de la precipitación. La respuesta sedimentológica está principalmente controlada por la dinámica espacio-temporal de las áreas fuente de escorrentía y de sedimento. La exportación de sedimento ilustra la complejidad de la respuesta geomorfológica en los ambientes de montaña afectados por actividades agrarias en el pasado.

  10. The "bear" essentials: actualistic research on Ursus arctos arctos in the Spanish Pyrenees and its implications for paleontology and archaeology.

    Science.gov (United States)

    Arilla, Maite; Rosell, Jordi; Blasco, Ruth; Domínguez-Rodrigo, Manuel; Pickering, Travis Rayne

    2014-01-01

    Neotaphonomic studies of large carnivores are used to create models in order to explain the formation of terrestrial vertebrate fossil faunas. The research reported here adds to the growing body of knowledge on the taphonomic consequences of large carnivore behavior in temperate habitats and has important implications for paleontology and archaeology. Using photo- and videotrap data, we were able to describe the consumption of 17 ungulate carcasses by wild brown bears (Ursus arctos arctos) ranging the Spanish Pyrenees. Further, we analyzed the taphonomic impact of these feeding bouts on the bones recovered from those carcasses. The general sequence of consumption that we charted starts with separation of a carcass's trunk; viscera are generally eaten first, followed by musculature of the humerus and femur. Long limb bones are not broken open for marrow extraction. Bears did not transport carcasses or carcass parts from points of feeding and did not disperse bones appreciably (if at all) from their anatomical positions. The general pattern of damage that resulted from bear feeding includes fracturing, peeling, crenulation, tooth pitting and scoring of axial and girdle elements and furrowing of the upper long limb bones. As predicted from observational data, the taphonomic consequences of bear feeding resemble those of other non-durophagus carnivores, such as felids, and are distinct from those of durophagus carnivores, such as hyenids. Our results have paleontological and archaeological relevance. Specifically, they may prove useful in building analogical models for interpreting the formation of fossil faunas for which bears are suspected bone accumulators and/or modifiers. More generally, our comparative statistical analyses draw precise quantitative distinctions between bone damage patterns imparted respectively by durophagus (modelled here primarily by spotted hyenas [Crocuta crocuta] and wolves [Canis lupus]) and non-durophagus (modelled here by brown bears and

  11. Small-Scale Effect of Pine Stand Pruning on Snowpack Distribution in the Pyrenees Observed with a Terrestrial Laser Scanner

    Directory of Open Access Journals (Sweden)

    Jesús Revuelto

    2016-07-01

    Full Text Available Forests in snow-dominated areas have substantial effects on the snowpack and its evolution over time. Such interactions have significant consequences for the hydrological response of mountain rivers. Thus, the impact of forest management actions on the snow distribution, and hence the storage of water in the form of snow during winter and spring, is a major concern. The results of this study provide the first detailed comparison of the small-scale effect of forest characteristics on the snowpack distribution, assessed prior to and following major modification of the structure of the canopy by pruning of the lower branches of the trees to 3 m above the ground. This is a common management practice aimed at reducing the spread of forest fires. The snowpack distribution was determined using terrestrial laser scanning (LiDAR technology at a high spatial resolution (0.25 m over a 1000 m2 study area during 23 survey dates over three snow seasons in a small study area in the central Pyrenees. The pruning was conducted during summer following the snow season in the second year of the study (i.e., the study duration encompassed two seasons prior to canopy pruning and one following. Principal component analysis (PCA was used to identify recurring spatial patterns of snow distribution. The results showed that pruning reduced the average radius of the canopy of trees by 1.2 m, and increased the clearance around the trunks, as all the branches that formerly contacted the ground were removed. However, the impact on the snowpack was moderate. The PCA revealed that the spatial configuration of the snowpack did not change significantly, as the principal components included survey days from different periods of the snow season, and did not discriminate days surveyed prior to and following pruning. Nevertheless, removal of the lower branches reduced the area beneath the canopy by 36%, and led to an average increase in total snow depth of approximately 14%.

  12. Lithology controls the regional distribution and morphological diversity of montane Mediterranean badlands in the upper Llobregat basin (eastern Pyrenees)

    Science.gov (United States)

    Moreno-de las Heras, Mariano; Gallart, Francesc

    2016-11-01

    Badlands are pervasive in a wide range of environmental conditions across the Mediterranean region, including arid, semiarid, and humid environments. On montane (cold subhumid and humid) Mediterranean landscapes, harsh thermal conditions on north-facing hillslopes favour intense bedrock weathering by freezing and impose serious constraints on plant colonization. The above are the customary arguments to explain the high abundance of montane badlands on north-exposed shady aspects. We studied the distribution and morphological diversity of badlands in the upper Llobregat basin (Catalan Pyrenees, NE Spain), using remote sensing information (high-resolution orthophotos and complementary Landsat 8 imagery), digital elevation data, and regional information on lithology. Badlands extend over about 200 ha in the upper Llobregat basin and occur on two different parent materials, swelling (smectite-rich) continental Garumnian lutites of late Cretaceous age, and nonswelling (illitic) marine Eocene marls. Vegetation, assessed by remote-sensed vegetation greenness, is less developed on north-facing badland slopes. However, badland slope-aspect distribution varies considerably with lithology. While badlands on Eocene marls show preferential distribution on north-facing shady slopes, badland occurrence on Garumnian lutites does not reveal clear slope-aspect anisotropy patterns. Lithology also affects slope gradient, with badlands on Eocene marls showing rougher topography (30-40° average slope angle) than on Garumnian lutites (20-30°). Badland morphological differences induced by lithology are discussed in terms of the greater weathering susceptibility and slope instability of the swelling, smectite-rich Garumnian lutites than of the nonswelling Eocene marls. Elevation, which broadly controls annual precipitation and winter air temperature within the region, shows no clear influence on badland distribution. Overall, our results reveal lithology as the main factor controlling

  13. Antigen sparing and enhanced protection using a novel rOv-ASP-1 adjuvant in aqueous formulation with influenza vaccines.

    Science.gov (United States)

    Jiang, Jiu; Fisher, Erin M; Hensley, Scott E; Lustigman, Sara; Murasko, Donna M; Shen, Hao

    2014-05-13

    Influenza is one of the most common infectious diseases endangering the health of humans, especially young children and the elderly. Although vaccination is the most effective means of protection against influenza, frequent mutations in viral surface antigens, low protective efficacy of the influenza vaccine in the elderly, slow production process and the potential of vaccine supply shortage during a pandemic are significant limitations of current vaccines. Adjuvants have been used to enhance the efficacy of a variety of vaccines; however, no adjuvant is included in current influenza vaccines approved in the United States. In this study, we found that a novel adjuvant, rOv-ASP-1, co-administrated with inactivated influenza vaccine using an aqueous formulation, substantially improved the influenza-specific antibody response and protection against lethal infection in a mouse model. rOv-ASP-1 enhanced the magnitude of the specific antibody response after immunization with low doses of influenza vaccine, allowing antigen-sparring by 10-fold. The rOv-ASP-1 formulated vaccine induced a more rapid response and a stronger Th1-associated antibody response compared to vaccine alone and to the vaccine formulated with the adjuvant alum. Importantly, rOv-ASP-1 significantly enhanced cross-reactive antibody responses and protection against challenge with an antigenically distinct strain. These results demonstrate that rOv-ASP-1 is an effective adjuvant that: (1) accelerates and enhances the specific antibody response induced by influenza vaccine; (2) allows for antigen sparing; and (3) augments a Th1-biased and cross-reactive antibody response that confers protection against an antigenically distinct strain. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Coordination ability determined transition metal ions substitution of Tb in Tb-Asp fluorescent nanocrystals and a facile ions-detection approach.

    Science.gov (United States)

    Duan, Jiazhi; Ma, Baojin; Liu, Feng; Zhang, Shan; Wang, Shicai; Kong, Ying; Du, Min; Han, Lin; Wang, Jianjun; Sang, Yuanhua; Liu, Hong

    2018-04-11

    Although the synthesis and fluorescent properties of lanthanide-amino acid complex nanostructures have been investigated extensively, limited studies have been reported on metal ions' substitution ability for the lanthanide ions in the complex and their effect on the fluorescent property. In this study, taking biocompatible Tb-aspartic acid (Tb-Asp) complex nanocrystals as a model, the substitution mechanism of metal ions, particularly transition metals, for Tb ions in Tb-Asp nanocrystals and the change in the fluorescent property of the Tb-Asp nanocrystals after substitution were systematically investigated. The experimental results illustrated that metal ions with higher electronegativity, higher valence, and smaller radius possess stronger ability for Tb ions' substitution in Tb-Asp nanocrystals. Based on the effect of substituting ions' concentration on the fluorescent property of Tb-Asp, a facile method for copper ions detection with high sensitivity was proposed by measuring the fluorescent intensity of Tb-Asp nanocrystals' suspensions containing different concentrations of copper ions. The good biocompatibility, great convenience of synthesis and sensitive detection ability make Tb-Asp nanocrystals a very low cost and effective material for metal ions detection, which also opens a new door for practical applications of metal-Asp coordinated nanocrystals.

  15. Topology of AspT, the Aspartate:Alanine Antiporter of Tetragenococcus halophilus, Determined by Site-Directed Fluorescence Labeling▿ †

    Science.gov (United States)

    Nanatani, Kei; Fujiki, Takashi; Kanou, Kazuhiko; Takeda-Shitaka, Mayuko; Umeyama, Hideaki; Ye, Liwen; Wang, Xicheng; Nakajima, Tasuku; Uchida, Takafumi; Maloney, Peter C.; Abe, Keietsu

    2007-01-01

    The gram-positive lactic acid bacterium Tetragenococcus halophilus catalyzes the decarboxylation of l-aspartate (Asp) with release of l-alanine (Ala) and CO2. The decarboxylation reaction consists of two steps: electrogenic exchange of Asp for Ala catalyzed by an aspartate:alanine antiporter (AspT) and intracellular decarboxylation of the transported Asp catalyzed by an l-aspartate-β-decarboxylase (AspD). AspT belongs to the newly classified aspartate:alanine exchanger family (transporter classification no. 2.A.81) of transporters. In this study, we were interested in the relationship between the structure and function of AspT and thus analyzed the topology by means of the substituted-cysteine accessibility method using the impermeant, fluorescent, thiol-specific probe Oregon Green 488 maleimide (OGM) and the impermeant, nonfluorescent, thiol-specific probe [2-(trimethylammonium)ethyl]methanethiosulfonate bromide. We generated 23 single-cysteine variants from a six-histidine-tagged cysteineless AspT template. A cysteine position was assigned an external location if the corresponding single-cysteine variant reacted with OGM added to intact cells, and a position was assigned an internal location if OGM labeling required cell lysis. The topology analyses revealed that AspT has a unique topology; the protein has 10 transmembrane helices (TMs), a large hydrophilic cytoplasmic loop (about 180 amino acids) between TM5 and TM6, N and C termini that face the periplasm, and a positively charged residue (arginine 76) within TM3. Moreover, the three-dimensional structure constructed by means of the full automatic modeling system indicates that the large hydrophilic cytoplasmic loop of AspT possesses a TrkA_C domain and a TrkA_C-like domain and that the three-dimensional structures of these domains are similar to each other even though their amino acid sequences show low similarity. PMID:17660287

  16. [Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis].

    Science.gov (United States)

    Skochko, O V; Bobrova, N A; Izmaylova, O V; Kaĭdashev, I P

    2011-01-01

    Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD). Samples of coronary vessels obtained during autopsy of 31 individuals deceased from IHD and 5 individuals deceased due to reasons not related with IHD were studied. PCR was used to determine DNA of the microorganisms. TLR4 gene polymorphic segment was amplified by using specific primers. Analysis of coronary vessel atherosclerotic plaques revealed presence of the studied periodontopathogenic microorganisms in 83.9% of cases. The most frequently detected were Porphyromonas gingivalis (64.5%), Treponema denticola (41.9%), Actinobacillus actinomycetemcomitans (32.3%), less frequently--Bacteroides forsythus and Prevotella intermedia (12.9% and 6.5% respectively). In 51.6% of cases 2 or more microorganisms were detected. Only in 11.1% ofcoronary artery samples, with plaques containing microorganisms, the microorganisms were detected in undamaged tissues. Patients deceased from IHD had TLR4 gene 299Gly allele significantly more frequently. The studied periodontopathogenic microorganisms can play an important role in the pathogenesis of atherosclerotic injury of coronary arteries in IHD. The presence of TLR4 gene allele 299Gly significantly contributes to these processes.

  17. Seismic image of the Cantabrian Mountains in the western extension of the Pyrenees from integrated ESCIN reflection and refraction data

    Science.gov (United States)

    Pulgar, J. A.; Gallart, J.; Fernández-Viejo, G.; Pérez-Estaún, A.; Álvarez-Marrón, J.; Escin Group

    1996-10-01

    different thicknesses. This signature offers a strong parallelism with the one previously observed further east across the Pyrenees in the ECORS seismic profile.

  18. Some learnings from post-event field investigations after the june 2013 floods in the Pyrenees region in France.

    Science.gov (United States)

    Payrastre, Olivier; Bonnifait, Laurent; Gaume, Eric; Le Boursicaut, Raphael

    2014-05-01

    In June 2013 catastrophic floods occurred in south of France in the Pyrenees mountainous area. These floods were due to the combination of a high initial discharge due to snowmelt with a significant rainfall event (up to 200mm rainfall), which effects may have been enhanced by an increase of snowmelt. Although the dynamics of this flood are not really similar, some of its features clearly remind what may be observed in the case of flash floods: significant contribution of relatively small watersheds, high solid transport, very limited information on the reality of flood magnitudes due to the small size of catchments contributing to the flood and the destruction of a significant part of the gauging network. This contribution presents the results of a post event field survey conducted in July 2013 in order to document this flood in terms of intensities of hydrologic reactions. The methods used are those described in Gaume et al. [2008, 2009], with a specific focus on the exploitation of videos from weatnesses. The dataset builded includes 31 peak discharge estimates, illustrating the relatively limited intensity of hydrologic reactions if compared to flash floods, but also providing some interesting complements for the consolidation of the methodology used for post-event field investigations: - several opportunities of comparison of the peak discharge estimates obtained from post event field investigations and from the gauging network, showing an overall good coherence - possibility of very significant flow velocities (up to 6 m/s-2) in the specific context observed here (slopes reaching up to 5%). - possibility to get information on flow surface velocities fields from videos provided by weatnesses. - significant influence of space-time rainfall distribution on the features of the flood, stressing the importance of a detailed information on the contribution of the sub-catchments. Gaume E., Borga M., 2008. Post flood field investigations after major flash floods

  19. Potential of airborne LiDAR data analysis to detect subtle landforms of slope failure: Portainé, Central Pyrenees

    Science.gov (United States)

    Ortuño, María; Guinau, Marta; Calvet, Jaume; Furdada, Glòria; Bordonau, Jaume; Ruiz, Antonio; Camafort, Miquel

    2017-10-01

    Slope failures have been traditionally detected by field inspection and aerial-photo interpretation. These approaches are generally insufficient to identify subtle landforms, especially those generated during the early stages of failures, and particularly where the site is located in forested and remote terrains. We present the identification and characterization of several large and medium size slope failures previously undetected within the Orri massif, Central Pyrenees. Around 130 scarps were interpreted as being part of Rock Slope Failures (RSFs), while other smaller and more superficial failures were interpreted as complex movements combining colluvium slow flow/slope creep and RSFs. Except for one of them, these slope failures had not been previously detected, albeit they extend across a 15% of the studied region. The failures were identified through the analysis of a high-resolution (1 m) LIDAR-derived bare earth Digital Elevation Model (DEM). Most of the scarps are undetectable either by fieldwork, photo interpretation or 5 m resolution topography analysis owing to their small heights (0.5 to 2 m) and their location within forest areas. In many cases, these landforms are not evident in the field due to the presence of other minor irregularities in the slope and the lack of open views due to the forest. 2D and 3D visualization of hillshade maps with different sun azimuths provided an overall picture of the scarp assemblage and permitted a more complete analysis of the geometry of the scarps with respect to the slope and the structural fabric. The sharpness of some of the landforms suggests ongoing activity, which should be explored in future detailed studies in order to assess potential hazards affecting the Portainé ski resort. Our results reveal that close analysis of the 1 m LIDAR-derived DEM can significantly help to detect early-stage slope deformations in high mountain regions, and that expert judgment of the DEM is essential when dealing with subtle

  20. Salt structures and vertical axis rotations; a case study in the Barbastro-Balaguer anticline, Southern Pyrenees.

    Science.gov (United States)

    Pueyo, Emilio L.; Oliván, Carlota; Soto, Ruth; Rodríguez-Pintó, Adriana; Santolaria, Pablo; Luzón, Aránzazu; Casas, Antonio M.; Ayala, Conxi

    2017-04-01

    Vertical axis rotations are common in all deformation settings. At larger scales, for example in fold and thrust belts, they are usually related to differential shortening along strike and this may be caused by a number of reasons (interplay of plate boundaries, sedimentary wedges, detachment level distribution, etc.). At smaller scales, local stress fields, interference of non-coaxial deformation phases, development of non-cylindrical structures, etc. may play an important role to accommodate significant magnitudes of rotation. Apart from their implication in the truly 4D understanding of geological structures, the occurrence of vertical axis rotation usually precludes the application of most 3D restoration techniques and thus, increases the uncertainty in any 3D reconstruction. Salt structures may form in different geological settings, but focusing on compressive regimes, very little is known about the relation between their geometry and kinematics and their ability to accommodate vertical axis rotations (i.e. local or regional lateral gradients of shortening). The Barbastro-Balaguer anticline (BBA) is the southernmost structure of the Central Pyrenees. It is a large detachment fold spreading more than 150 km along the front. In contrast to most frontal Pyrenean structures, the BBA is detached in Priabonian evaporites and was folded during Oligocene times as witnessed by well exposed growth strata. Along strike changes in the fold axis trend may reach 50°, an overall the anticline displays a convex shape towards the foreland (south). A residual Bouguer anomaly map based on a densely sampled gravimetric surveying (10.000 stations) has helped delineating a heterogeneous distribution of the Eocene detachment level in the subsurface. In this contribution we explore the interplay between vertical axis rotations, detachment level distribution and the fold geometry (structural trend and style based on hundreds of data). Seventy paleomagnetic sites evenly and densely

  1. Holocene climate variability, vegetation dynamics and fire regime in the central Pyrenees: the Basa de la Mora sequence (NE Spain)

    Science.gov (United States)

    Pérez-Sanz, A.; González-Sampériz, P.; Moreno, A.; Valero-Garcés, B.; Gil-Romera, G.; Rieradevall, M.; Tarrats, P.; Lasheras-Álvarez, L.; Morellón, M.; Belmonte, A.; Sancho, C.; Sevilla-Callejo, M.; Navas, A.

    2013-08-01

    High resolution multiproxy data (pollen, sedimentology, geochemistry, chironomids and charcoal) from the Basa de la Mora (BSM) lake sequence (42° 32' N, 0° 19' E, 1914 m a.s.l.) show marked climate variability in the central southern Pyrenees throughout the Holocene. A robust age model based on 15 AMS radiocarbon dates underpins the first precise reconstruction of rapid climate changes during the Holocene from this area. During the Early Holocene, increased winter snowpack and high snowmelt during summer, as a consequence of high seasonality, led to higher lake levels, a chironomid community dominated by non-lacustrine taxa (Orthocladiinae) related to higher inlet streams, and a forested landscape with intense run-off processes in the watershed. From 9.8 to 8.1 cal ka BP, climate instability is inferred from rapid and intense forest shifts and high fluctuation in surface run-off. Shifts among conifers and mesophytes reveal at least four short-lived dry events at 9.7, 9.3, 8.8 and 8.3 cal ka BP. Between 8.1 and 5.7 cal ka BP a stable climate with higher precipitation favoured highest lake levels and forest expansion, with spread of mesophytes, withdrawal of conifers and intensification of fires, coinciding with the Holocene Climate Optimum. At 5.7 cal ka BP a major change leading to drier conditions contributed to a regional decline in mesophytes, expansion of pines and junipers, and a significant lake level drop. Despite drier conditions, fire activity dropped as consequence of biomass reduction. Two arid intervals occurred between 2.9 and 2.4 cal ka BP and at 1.2-0.7 cal ka BP (800-1300 AD). The latter coincides with the Medieval Climate Anomaly and is one of the most arid phases of the Holocene in BSM sequence. Anthropogenic disturbances were small until 700 AD, when human pressure over landscape intensified, with Olea cultivation in the lowlands and significant deforestation in highlands. Colder and unfavourable weather conditions during the second part of the

  2. Valley Fever (Coccidioidomycosis) Risk and Prevention

    Science.gov (United States)

    ... valley fever, but it is not contagious between animals and people. Valley fever in dogs is similar to valley ... Via Growth on Fomites. An Epidemic Involving Six Persons. Am Rev Respir Dis. ... aspects of coccidioidomycosis in animals and humans. Ann N Y Acad Sci. 2007 ...

  3. PENGEMBANGAN WEBSITE DINAMIS MENGGUNAKAN ASP.NET MVC DAN SQL SERVER DENGAN METODE RAD (STUDI KASUS: PT X

    Directory of Open Access Journals (Sweden)

    Fityan Aula Juyuspan

    2017-09-01

    Full Text Available Abstrak — Pembangunan website dinamis merupakan salah satu kebutuhan bagi beberapa perusahaan. Kebutuhan tersebut dapat berupa sebuah komunikasi dengan pelanggan, sebagai sarana informasi, promosi produk, statistic pengunjung, dan pengelolaan quotation. PT X merupakan sebuah perusahaan yang menjadikan website dinamis sebagai salah satu kebutuhan mereka. Website dinamis bertujuan untuk mengelola konten serta mempublikasikan promosi-promosi perusahaan sehingga mempermudah pengunjung dalam memahami dan berinteraksi aktif dengan perusahaan. Framework ASP.NET MVC merupakan salah satu metode yang dikembangkan oleh Microsoft yang digunakan untuk pembangunan website dinamis. Pada studi kasus ini bahasa pemrograman yang digunakan adalah C# dan menggunakan database SQL Server 2016. Tujuan dari pengembangan ini adalah untuk mengimplementasikan MVC Pattern, bundling and minification, cookie, AJAX (Asynchronous JavaScript and XML, dan Action Filter pada ASP.NET. Pengembangan tersebut menghasilkan website dinamis yang interaktif, menyediakan informasi dan layanan bagi pengunjung baru ataupun pelanggan dari perusahaan tersebut, serta dikembangkan dengan cepat (rapid dengan menggunakan metode RAD.

  4. Crystallization and preliminary X-ray analysis of Na-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite Necator americanus

    Energy Technology Data Exchange (ETDEWEB)

    Asojo, Oluwatoyin A., E-mail: oasojo@unmc.edu [Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696 (United States); Loukas, Alex [Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington DC 20037 (United States); Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, QLD 4006 (Australia); Inan, Mehmet; Barent, Rick; Huang, Jicai; Plantz, Brad; Swanson, Amber; Gouthro, Mark; Meagher, Michael M. [Department of Chemical Engineering, The University of Nebraska-Lincoln, Lincoln, NE 68588-0643 (United States); Hotez, Peter J. [Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington DC 20037 (United States); Eppley Institute for Research in Cancer and Allied Diseases, 987696 Nebraska Medical Center, Omaha, NE 68198-7696 (United States)

    2005-04-01

    In order to clarify the structural basis of the pathogenesis-related-1 domain, Na-ASP-1, the first multi-domain ASP from the human hookworm parasite N. americanus, has been crystallized. 2.2 Å resolution data have been collected from a crystal belonging to the monoclinic space group P2{sub 1}. Human hookworm infection is a major cause of anemia and malnutrition in the developing world. In an effort to control hookworm infection, the Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective larval stage (L3) of the parasite, including a family of pathogenesis-related-1 (PR-1) proteins known as the ancylostoma-secreted proteins (ASPs). The functions of the ASPs are unknown. In addition, it is unclear why some ASPs have one while others have multiple PR-1 domains. There are no known structures of a multi-domain ASP and in an effort to remedy this situation, recombinant Na-ASP-1 has been expressed, purified and crystallized. Na-ASP-1 is a 406-amino-acid multi-domain ASP from the prevalent human hookworm parasite Necator americanus. Useful X-ray data to 2.2 Å have been collected from a crystal that belongs to the monoclinic space group P2{sub 1} with unit-cell parameters a = 67.7, b = 74.27, c = 84.60 Å, β = 112.12°. An initial molecular-replacement solution has been obtained with one monomer in the asymmetric unit.

  5. Stability, metabolism and transport of D-Asp(OBzl)-Ala--a model prodrug with affinity for the oligopeptide transporter

    DEFF Research Database (Denmark)

    Steffansen, B; Lepist, E I; Taub, M E

    1999-01-01

    model. The aqueous stability studies show water, general buffer, as well as specific acid and base catalysis of D-Asp(OBzl)-Ala. The degradation of the model prodrug was independent of ionic strength. The half-lives in rat jejunal fluid and homogenate were >3 h. In human gastric and intestinal fluids...... jejunal oligopeptide transporter, since the stability in the intestinal homogenate and fluids was rather high (t1/2>2.3 h)....

  6. Interactions and Interventions: Current Research on Improving Informal Astronomy Education via the Astronomical Society of the Pacific (ASP)

    Science.gov (United States)

    Manning, James G.; Gurton, S.; Hurst, A.; Berendsen, M.; Storksdieck, M.; Haley-Goldman, K.; Stein, J.; Pompea, S.; Garmany, C.; Sparks, R.; Pollock, W.

    2007-12-01

    In building national capacity for better informal astronomy education and public outreach (EPO), what sorts of professional development interactions are most effective in what situations--and what interventions for improvement can be effectively applied? Building on previous experience, the ASP, in conjunction with its partners, is conducting two National Science Foundation (NSF) funded projects investigating astronomy teaching and learning in informal contexts to explore these questions in both museum-based and amateur astronomy club settings. "Astronomy from the Ground Up" (AfGU) develops capacity for hands-on astronomy education in small and medium-sized science centers and nature centers through on-site and online professional development workshops and the establishment of a "community of practice" network. The ASP, in collaboration with the National Optical Astronomy Observatory (NOAO) and the Association of Science and Technology Centers (ASTC), is investigating which model--face-to-face or online professional development--works best and will be sustainable for that target group. "Sharing the Universe" (STU) builds on the Night Sky Network in which amateur astronomy clubs, through the ASP with financial and logistical support from NASA and its missions, are provided tools and training to conduct EPO activities with their public audiences. The ASP, in collaboration with the Institute for Learning Innovation (ILI), launched a national survey in late 2007 to investigate the factors that either support or discourage sustained amateur astronomer EPO efforts, followed by an in-depth study of a subset of both successful and struggling clubs, and leading to the development of interventions that support amateur astronomy outreach within the context of a nurturing club environment. The presentation will offer some early and initial results of the AfGU project--which reveal some interesting and unforeseen advantages of the online model over the on-site model--and some

  7. Death adder envenoming causes neurotoxicity not reversed by antivenom--Australian Snakebite Project (ASP-16).

    Science.gov (United States)

    Johnston, Christopher I; O'Leary, Margaret A; Brown, Simon G A; Currie, Bart J; Halkidis, Lambros; Whitaker, Richard; Close, Benjamin; Isbister, Geoffrey K

    2012-01-01

    Death adders (Acanthophis spp) are found in Australia, Papua New Guinea and parts of eastern Indonesia. This study aimed to investigate the clinical syndrome of death adder envenoming and response to antivenom treatment. Definite death adder bites were recruited from the Australian Snakebite Project (ASP) as defined by expert identification or detection of death adder venom in blood. Clinical effects and laboratory results were collected prospectively, including the time course of neurotoxicity and response to treatment. Enzyme immunoassay was used to measure venom concentrations. Twenty nine patients had definite death adder bites; median age 45 yr (5-74 yr); 25 were male. Envenoming occurred in 14 patients. Two further patients had allergic reactions without envenoming, both snake handlers with previous death adder bites. Of 14 envenomed patients, 12 developed neurotoxicity characterised by ptosis (12), diplopia (9), bulbar weakness (7), intercostal muscle weakness (2) and limb weakness (2). Intubation and mechanical ventilation were required for two patients for 17 and 83 hours. The median time to onset of neurotoxicity was 4 hours (0.5-15.5 hr). One patient bitten by a northern death adder developed myotoxicity and one patient only developed systemic symptoms without neurotoxicity. No patient developed venom induced consumption coagulopathy. Antivenom was administered to 13 patients, all receiving one vial initially. The median time for resolution of neurotoxicity post-antivenom was 21 hours (5-168). The median peak venom concentration in 13 envenomed patients with blood samples was 22 ng/mL (4.4-245 ng/mL). In eight patients where post-antivenom bloods were available, no venom was detected after one vial of antivenom. Death adder envenoming is characterised by neurotoxicity, which is mild in most cases. One vial of death adder antivenom was sufficient to bind all circulating venom. The persistent neurological effects despite antivenom, suggests that

  8. Death adder envenoming causes neurotoxicity not reversed by antivenom--Australian Snakebite Project (ASP-16.

    Directory of Open Access Journals (Sweden)

    Christopher I Johnston

    Full Text Available BACKGROUND: Death adders (Acanthophis spp are found in Australia, Papua New Guinea and parts of eastern Indonesia. This study aimed to investigate the clinical syndrome of death adder envenoming and response to antivenom treatment. METHODOLOGY/PRINCIPAL FINDINGS: Definite death adder bites were recruited from the Australian Snakebite Project (ASP as defined by expert identification or detection of death adder venom in blood. Clinical effects and laboratory results were collected prospectively, including the time course of neurotoxicity and response to treatment. Enzyme immunoassay was used to measure venom concentrations. Twenty nine patients had definite death adder bites; median age 45 yr (5-74 yr; 25 were male. Envenoming occurred in 14 patients. Two further patients had allergic reactions without envenoming, both snake handlers with previous death adder bites. Of 14 envenomed patients, 12 developed neurotoxicity characterised by ptosis (12, diplopia (9, bulbar weakness (7, intercostal muscle weakness (2 and limb weakness (2. Intubation and mechanical ventilation were required for two patients for 17 and 83 hours. The median time to onset of neurotoxicity was 4 hours (0.5-15.5 hr. One patient bitten by a northern death adder developed myotoxicity and one patient only developed systemic symptoms without neurotoxicity. No patient developed venom induced consumption coagulopathy. Antivenom was administered to 13 patients, all receiving one vial initially. The median time for resolution of neurotoxicity post-antivenom was 21 hours (5-168. The median peak venom concentration in 13 envenomed patients with blood samples was 22 ng/mL (4.4-245 ng/mL. In eight patients where post-antivenom bloods were available, no venom was detected after one vial of antivenom. CONCLUSIONS/SIGNIFICANCE: Death adder envenoming is characterised by neurotoxicity, which is mild in most cases. One vial of death adder antivenom was sufficient to bind all circulating venom

  9. Arthropods (http://www.iaees.org/publications/journals/arthropods/online-version.asp

    Directory of Open Access Journals (Sweden)

    arthropods@iaees.org

    Full Text Available Arthropods ISSN 2224-4255 URL: http://www.iaees.org/publications/journals/arthropods/online-version.asp RSS: http://www.iaees.org/publications/journals/arthropods/rss.xml E-mail: arthropods@iaees.org Editor-in-Chief: WenJun Zhang Aims and Scope ARTHROPODS (ISSN 2224-4255 is an international journal devoted to the publication of articles on various aspects of arthropods, e.g., ecology, biogeography, systematics, biodiversity (species diversity, genetic diversity, et al., conservation, control, etc. The journal provides a forum for examining the importance of arthropods in biosphere (both terrestrial and marine ecosystems and human life in such fields as agriculture, forestry, fishery, environmental management and human health. The scope of Arthropods is wide and embraces all arthropods-insects, arachnids, crustaceans, centipedes, millipedes, and other arthropods. Articles/short communications on new taxa (species, genus, families, orders, etc. and new records of arthropods are particularly welcome. Authors can submit their works to the email box of this journal, arthropods@iaees.org. All manuscripts submitted to this journal must be previously unpublished and may not be considered for publication elsewhere at any time during review period of this journal. Authors are asked to read Author Guidelines before submitting manuscripts. In addition to free submissions from authors around the world, special issues are also accepted. The organizer of a special issue can collect submissions (yielded from a research project, a research group, etc. on a specific research topic, or submissions of a scientific conference for publication of special issue.

  10. Environmental Skeptics and Critics (http://www.iaees.org/publications/journals/environsc/online-version.asp

    Directory of Open Access Journals (Sweden)

    environsc@iaees.org

    Full Text Available Environmental Skeptics and Critics ISSN 2224-4263 URL: http://www.iaees.org/publications/journals/environsc/online-version.asp RSS: http://www.iaees.org/publications/journals/environsc/rss.xml E-mail: environsc@iaees.org Editor-in-Chief: WenJun Zhang Aims and Scope The more truth is debated, the clearer it becomes. Science will not proceed without debate and controversy. Wide and in-depth debate and controversy on human's knowledge, attitudes, policies and practices on the environment determines the future of our planet. There are numerous controversial and potentially controversial issues on environmental sciences and practices. ENVIRONMENTAL SKEPTICS and CRITICS (ISSN 2224-4263 is an international journal devoted to the publication of skeptical and critical articles/short communications/letters on theories, viewpoints, methodologies, practices, policies, etc., in ecological and environmental areas. The journal provides a forum for questioning, disputing, arguing, challenging, criticizing and judging known theories, methdologies, practices, and policies, etc., or presenting different ideas. The scope of Environmental Skeptics and Critics is wide and embraces all controversial, non-conclusive or unexplained issues in ecological and environmental areas. Authors can submit their works to the email box of this journal, environsc@iaees.org. All manuscripts submitted to this journal must be previously unpublished and may not be considered for publication elsewhere at any time during review period of this journal. Authors are asked to read Author Guidelines before submitting manuscripts. In addition to free submissions from authors around the world, special issues are also accepted. The organizer of a special issue can collect submissions (yielded from a research project, a research group, etc. on a specific research topic, or submissions of a scientific conference for publication of special issue.

  11. [Key role of Asp16 in proteolysis of influenza A NP protein by caspases in infected cells].

    Science.gov (United States)

    Zhirnov, O P; Vorob'eva, I V; Veselovskiĭ, E M; Klenk, N

    2003-01-01

    The main nucleocapsid protein NP (molecular weight--56 kD) of human influenza A virus (IAV) was found to be subject to the N-terminal proteolysis in position Asp16 with production of aNP (molecular weight--53 kD) in the infected cells' apoptosis. It was assumed that NP of avian and animal influenza viruses was not subject to proteolysis since it has Gly16. To verify the above assumption the NP chimeric gene of human influenza virus was developed; Asp16 was replaced by Gly by means of "site-oriented" mutagenesis in the above gene, after that, the A/WSN/33 (H1N1) mutant of human influenza virus with "avian" NP and with point mutation (Gly16) was developed by using the method of "reverse genetics". The "human" influenza virus with "avian" chimeric NP/Gly16 turned out to be viable but had a lower replication velocity versus its wild-nature counterpart. It is noteworthy, that the mutant virus caused the cellular apoptosis in the remote infection period the way the wild virus did; however, NP of the former was found to be resistant to cellular caspasas and was not subject to proteolysis in infected cells. The conclusion is that Asp16 in NP molecule of human IAV is involved into the regulation process of virus replication and is the key element in NP proteolysis by cellular caspasas in cells' apoptosis.

  12. Characterization of the glycosylation profiles of Alzheimer's beta -secretase protein Asp-2 expressed in a variety of cell lines.

    Science.gov (United States)

    Charlwood, J; Dingwall, C; Matico, R; Hussain, I; Johanson, K; Moore, S; Powell, D J; Skehel, J M; Ratcliffe, S; Clarke, B; Trill, J; Sweitzer, S; Camilleri, P

    2001-05-18

    Amyloid 39-42 beta -peptides are the main components of amyloid plaques found in the brain of Alzheimer's disease patients. Amyloid 39-42 beta-peptide is formed from amyloid precursor protein by the sequential action of beta- and gamma-secretases. Asp-2 is a transmembrane aspartic protease expressed in the brain, shown to have beta-secretase activity. Mature Asp-2 has four N-glycosylation sites. In this report we have characterized the carbohydrate structures in this glycoprotein expressed in three different cell lines, namely Chinese hamster ovary, CV-1 origin of SV40, and baculovirus-infected SF9 cells. Biantennary and triantennary oligosaccharides of the "complex" type were released from glycoprotein expressed in the mammalian cells, whereas mannose-rich glycans were identified from glycoprotein synthesized in the baculovirus-infected cells. Site-directed mutagenesis of the asparagine residues at amino acid positions 153, 172, 223, and 354 demonstrate that the protease activity of Asp-2 is dependent on its glycosylation.

  13. Formation of crystalline nanoparticles by iron binding to pentapeptide (Asp-His-Thr-Lys-Glu) from egg white hydrolysates.

    Science.gov (United States)

    Sun, Na; Cui, Pengbo; Li, Dongmei; Jin, Ziqi; Zhang, Shuyu; Lin, Songyi

    2017-09-20

    A novel peptide from egg white, Asp-His-Thr-Lys-Glu (DHTKE), contains specific amino acids associated with iron binding. The present study aims to better understand the molecular basis of interactions between the DHTKE peptide and iron ions. The ultraviolet-visible and fluorescence spectra indicate an interaction between the DHTKE peptide and iron ions, which leads to the formation of a DHTKE-iron complex. Notably, Asp, Glu, His, and Lys in the DHTKE peptide play crucial roles in the formation of the DHTKE-iron complex, and the iron-binding site of the DHTKE peptide corresponds primarily to the amide and carboxyl groups. The DHTKE peptide can bind iron ions in a 1 : 2 ratio with a binding constant of 1.312 × 10 5 M -1 . Moreover, the DHTKE-iron complex belongs to thermodynamically stable nanoparticles that are present in the crystalline structure, which might be attributed to peptide folding induced by iron binding. Meanwhile, the DHTKE-iron complex exhibits a relatively high iron-releasing percentage and exerts excellent solubility in the human gastrointestinal tract in vitro. This suggests a potential application of peptides containing Asp, Glu, His, or Lys residues as potential iron supplements.

  14. Screening of Pro-Asp Sequences Exposed on Bacteriophage M13 as an Ideal Anchor for Gold Nanocubes.

    Science.gov (United States)

    Lee, Hwa Kyoung; Lee, Yujean; Kim, Hyori; Lee, Hye-Eun; Chang, Hyejin; Nam, Ki Tae; Jeong, Dae Hong; Chung, Junho

    2017-09-15

    Bacteriophages are thought to be ideal vehicles for linking antibodies to nanoparticles. Here, we define the sequence of peptides exposed as a fusion protein on M13 bacteriophages to yield optimal binding of gold nanocubes and efficient bacteriophage amplification. We generated five helper bacteriophage libraries using AE(X) 2 DP, AE(X) 3 DP, AE(X) 4 DP, AE(X) 5 DP, and AE(X) 6 DP as the exposed portion of pVIII, in which X was a randomized amino acid residue encoded by the nucleotide sequence NNK. Efficient phage amplification was achievable only in the AE(X) 2 DP, AE(X) 3 DP, and AE(X) 4 DP libraries. Through biopanning with gold nanocubes, we enriched the phage clones and selected the clone with the highest fold change after enrichment. This clone displayed Pro-Asp on the surface of the bacteriophage and had amplification yields similar to those of the wild-type helper bacteriophage (VCSM13). The clone displayed even binding of gold nanocubes along its length and minimal aggregation after binding. We conclude that, for efficient amplification, the exposed pVIII amino acid length should be limited to six residues and Ala-Glu-Pro-Asp-Asp-Pro (AEPDDP) is the ideal fusion protein sequence for guaranteeing the optimal formation of a complex with gold nanocubes.

  15. Physico-chemical study of the ancient and recent accumulations of a braided system (Ara river. Central Pyrenees, Spain

    Directory of Open Access Journals (Sweden)

    Rubio, V.

    1997-12-01

    Full Text Available The physico-chemical and mineralogical study of the Ara river sediments, in the Central Pyrenees (Spain, allows to recognize the genesis and evolution of its basin. The minerals of the sand fraction, the ones corresponding to the clay fraction and the minority elements, reveal certain processes and associations that, in addition to the identification of the fluvial terraces, fans, glacis and even morainic deposits, contribute to explain the geological history of the Central Pyrenees, the main objective of this work. The application of a multivariate factorial analysis to the data with BMDP-4M program was very helpful for the interpretation of the results. The more resistant minerals are found in the thinner textural fractions, whereas the more alterable are located in the thicker fractions. The cones and glacis, composed by materials of the flysch, reveal an alteration of the carbonated materials which is shown by the formation of feldspars and anphybols. The formation of iron and manganese metal oxides, of different crystallinity degrees, associated with chrome, lead and nickel, is also observed. The morainic deposits, mainly composed by silicate materials, bring along the formation of illite associated with copper and zinc. Finally, the fluvial terraces have a combination of both alteration processes previously described.

    [es] El estudio físico-químico y mineralógico de los sedimentos del río Ara, en el Pirineo Central (España, permite reconocer la génesis y evolución de su cuenca. Los minerales de la fracción arena, los correspondientes a la fracción arcilla y los elementos minoritarios, delatan ciertos procesos y asociaciones que, junto con la identificación de terrazas fluviales, conos, glacis e incluso depósitos morrénicos, ayudan a explicar la historia geológica del Pirineo Central, principal objetivo de este trabajo. La aplicación al conjunto de datos de un análisis factorial multivariante mediante el programa BMDP

  16. Snow cover dynamics in the Catalan Pyrenees range using remote sensing data from 2002 to 2008 period

    Science.gov (United States)

    Cea, C.; Cristóbal, J.; Pons, X.

    2009-04-01

    Snow cover dynamics in the Catalan Pyrenees range using remote sensing data from 2002 to 2008 period. C. Cea (1), J. Cristóbal (1), X. Pons (1, 2) (1) Department of Geography. Autonomous University of Barcelona. Cerdanyola del Vallès, 08193. Cristina.Cea@uab.cat, (2) Center for Ecological Research and Forestry Applications (CREAF) Cerdanyola del Vallès, 08193. Water resources and its management are essential in many alpine mountainous areas. Snow cover monitoring in the Mediterranean zone requires obtaining accurate snow cartography to estimate the volume of water derived from snow melting and species distribution modelling. Snow data is usually obtained by field campaigns, but to obtain a spatial and temporal cover of enough detail and quality it is necessary collect an important number of data. However, when a continuous surface is needed, Remote Sensing could provide better snow cover estimation due to its spatial and temporal resolution. The aim of this study is to map snow cover and analyse its spatial and temporal dynamics using medium and coarse remote sensing data at a regional scale over an heterogeneous area, the Catalan Pyrenees (NE of the Iberian Peninsula). The seasonal snow cover period is from October to June. In this period, regular snowfalls usually take place from December to April, although during the rest of the period, punctual but important episodes of snowfalls are frequent. To perform this analysis, a set of 96 Landsat images (36 Landsat-5 TM and 60 Landsat-7 ETM+) of path 197 and 198 and rows 31 and 32 from January 2002 to April 2007, and 90 Terra-MODIS images from October 2007 to July 2008, with a different percentage of cloudiness, have been chosen. The computation of the Landsat-5 TM and Landsat-7 ETM+ data used in snow cover mapping has been carried out by means of the following methodologies. Images have been geometrically corrected by means of techniques based on first order polynomials taking into account the effect of the relief

  17. Computational Ecology and Software (http://www.iaees.org/publications/journals/ces/online-version.asp

    Directory of Open Access Journals (Sweden)

    ces@iaees.org

    Full Text Available Computational Ecology and Software ISSN 2220-721X URL: http://www.iaees.org/publications/journals/ces/online-version.asp RSS: http://www.iaees.org/publications/journals/ces/rss.xml E-mail: ces@iaees.org Editor-in-Chief: WenJun Zhang Aims and Scope COMPUTATIONAL ECOLOGY AND SOFTWARE (ISSN 2220-721X is an open access, peer-reviewed online journal that considers scientific articles in all different areas of computational ecology. It is the transactions of the International Society of Computational Ecology. The journal is concerned with the ecological researches, constructions and applications of theories and methods of computational sciences including computational mathematics, computational statistics and computer science. It features the simulation, approximation, prediction, recognition, and classification of ecological issues. Intensive computation is one of the major stresses of the journal. The journal welcomes research articles, short communications, review articles, perspectives, and book reviews. The journal also supports the activities of the International Society of Computational Ecology. The topics to be covered by CES include, but are not limited to: •Computation intensive methods, numerical and optimization methods, differential and difference equation modeling and simulation, prediction, recognition, classification, statistical computation (Bayesian computing, randomization, bootstrapping, Monte Carlo techniques, stochastic process, etc., agent-based modeling, individual-based modeling, artificial neural networks, knowledge based systems, machine learning, genetic algorithms, data exploration, network analysis and computation, databases, ecological modeling and computation using Geographical Information Systems, satellite imagery, and other computation intensive theories and methods. •Artificial ecosystems, artificial life, complexity of ecosystems and virtual reality. •The development, evaluation and validation of software and

  18. Network Biology (http://www.iaees.org/publications/journals/nb/online-version.asp

    Directory of Open Access Journals (Sweden)

    networkbiology@iaees.org

    Full Text Available Network Biology ISSN 2220-8879 URL: http://www.iaees.org/publications/journals/nb/online-version.asp RSS: http://www.iaees.org/publications/journals/nb/rss.xml E-mail: networkbiology@iaees.org Editor-in-Chief: WenJun Zhang Aims and Scope NETWORK BIOLOGY (ISSN 2220-8879; CODEN NBEICS is an open access, peer-reviewed international journal that considers scientific articles in all different areas of network biology. It is the transactions of the International Society of Network Biology. It dedicates to the latest advances in network biology. The goal of this journal is to keep a record of the state-of-the-art research and promote the research work in these fast moving areas. The topics to be covered by Network Biology include, but are not limited to: •Theories, algorithms and programs of network analysis •Innovations and applications of biological networks •Ecological networks, food webs and natural equilibrium •Co-evolution, co-extinction, biodiversity conservation •Metabolic networks, protein-protein interaction networks, biochemical reaction networks, gene networks, transcriptional regulatory networks, cell cycle networks, phylogenetic networks, network motifs •Physiological networks •Network regulation of metabolic processes, human diseases and ecological systems •Social networks, epidemiological networks •System complexity, self-organized systems, emergence of biological systems, agent-based modeling, individual-based modeling, neural network modeling, and other network-based modeling, etc. We are also interested in short communications that clearly address a specific issue or completely present a new ecological network, food web, or metabolic or gene network, etc. Authors can submit their works to the email box of this journal, networkbiology@iaees.org. All manuscripts submitted to this journal must be previously unpublished and may not be considered for publication elsewhere at any time during review period of this journal

  19. Identification of active erosion areas and areas at risk by remote sensing: an example in the Esera Isabena watershed, Central Spanish Pyrenees

    International Nuclear Information System (INIS)

    Alatorre, L. C.; Begueria, S.; Vicente Serrano, S. M.

    2009-01-01

    The identification of eroded areas at basin scale can be very useful for environmental planning and can help to reduce land degradation and sediments yield. In this paper remote sensing technique are used to discriminate eroded areas and areas at risk in a badlands landscape developed on Eocene marls. In the Esera Isabena watershed (Spanish Pyrenees). The spatial distribution, the scarce vegetal cover and the high level of erosion let a good visual and digital discrimination of badlands, as opposed to other land covers and surfaces. A maximum likelihood supervised method was used to discriminate heavily eroded areas (badlands) from scarce or densely vegetated lands. the classification distance was used to obtain thresholds for eroded areas and areas at risk. Two error statistics (sensitivity and specificity), where used to determine the most adequate threshold values. The resulting map shows that most areas at risk are located surrounding the badlands areas. (Author) 8 refs.

  20. Synergism between basic Asp49 and Lys49 phospholipase A2 myotoxins of viperid snake venom in vitro and in vivo.

    Directory of Open Access Journals (Sweden)

    Diana Mora-Obando

    Full Text Available Two subtypes of phospholipases A2 (PLA2s with the ability to induce myonecrosis, 'Asp49' and 'Lys49' myotoxins, often coexist in viperid snake venoms. Since the latter lack catalytic activity, two different mechanisms are involved in their myotoxicity. A synergism between Asp49 and Lys49 myotoxins from Bothrops asper was previously observed in vitro, enhancing Ca2+ entry and cell death when acting together upon C2C12 myotubes. These observations are extended for the first time in vivo, by demonstrating a clear enhancement of myonecrosis by the combined action of these two toxins in mice. In addition, novel aspects of their synergism were revealed using myotubes. Proportions of Asp49 myotoxin as low as 0.1% of the Lys49 myotoxin are sufficient to enhance cytotoxicity of the latter, but not the opposite. Sublytic amounts of Asp49 myotoxin also enhanced cytotoxicity of a synthetic peptide encompassing the toxic region of Lys49 myotoxin. Asp49 myotoxin rendered myotubes more susceptible to osmotic lysis, whereas Lys49 myotoxin did not. In contrast to myotoxic Asp49 PLA2, an acidic non-toxic PLA2 from the same venom did not markedly synergize with Lys49 myotoxin, revealing a functional difference between basic and acidic PLA2 enzymes. It is suggested that Asp49 myotoxins synergize with Lys49 myotoxins by virtue of their PLA2 activity. In addition to the membrane-destabilizing effect of this activity, Asp49 myotoxins may generate anionic patches of hydrolytic reaction products, facilitating electrostatic interactions with Lys49 myotoxins. These data provide new evidence for the evolutionary adaptive value of the two subtypes of PLA2 myotoxins acting synergistically in viperid venoms.

  1. Synergism between Basic Asp49 and Lys49 Phospholipase A2 Myotoxins of Viperid Snake Venom In Vitro and In Vivo

    Science.gov (United States)

    Mora-Obando, Diana; Fernández, Julián; Montecucco, Cesare; Gutiérrez, José María; Lomonte, Bruno

    2014-01-01

    Two subtypes of phospholipases A2 (PLA2s) with the ability to induce myonecrosis, ‘Asp49’ and ‘Lys49’ myotoxins, often coexist in viperid snake venoms. Since the latter lack catalytic activity, two different mechanisms are involved in their myotoxicity. A synergism between Asp49 and Lys49 myotoxins from Bothrops asper was previously observed in vitro, enhancing Ca2+ entry and cell death when acting together upon C2C12 myotubes. These observations are extended for the first time in vivo, by demonstrating a clear enhancement of myonecrosis by the combined action of these two toxins in mice. In addition, novel aspects of their synergism were revealed using myotubes. Proportions of Asp49 myotoxin as low as 0.1% of the Lys49 myotoxin are sufficient to enhance cytotoxicity of the latter, but not the opposite. Sublytic amounts of Asp49 myotoxin also enhanced cytotoxicity of a synthetic peptide encompassing the toxic region of Lys49 myotoxin. Asp49 myotoxin rendered myotubes more susceptible to osmotic lysis, whereas Lys49 myotoxin did not. In contrast to myotoxic Asp49 PLA2, an acidic non-toxic PLA2 from the same venom did not markedly synergize with Lys49 myotoxin, revealing a functional difference between basic and acidic PLA2 enzymes. It is suggested that Asp49 myotoxins synergize with Lys49 myotoxins by virtue of their PLA2 activity. In addition to the membrane-destabilizing effect of this activity, Asp49 myotoxins may generate anionic patches of hydrolytic reaction products, facilitating electrostatic interactions with Lys49 myotoxins. These data provide new evidence for the evolutionary adaptive value of the two subtypes of PLA2 myotoxins acting synergistically in viperid venoms. PMID:25290688

  2. Elevated serum levels of caspase-cleaved cytokeratin 18 (CK18-Asp396) in patients with nonalcoholic steatohepatitis and chronic hepatitis C.

    Science.gov (United States)

    Yilmaz, Yusuf; Dolar, Enver; Ulukaya, Engin; Akgoz, Semra; Keskin, Murat; Kiyici, Murat; Yerci, Omer; Oral, Arzu Yilmaztepe; Gul, Cuma Bulent; Gurel, Selim; Nak, Selim Giray; Gulten, Macit

    2009-04-01

    Caspase-cleaved cytokeratin 18 (CK18-Asp396) is released from hepatocytes during apoptosis. Recent studies have indicated that serum levels of CK18-Asp396 could be a clinically useful biomarker of chronic liver disease. To shed more light on the rate of hepatocyte loss by apoptosis in chronic liver disease, serum levels of CK18-Asp396 were examined in patients with nonalcoholic steatohepatitis (NASH) and chronic hepatitis C. Apoptotic CK18-Asp396 levels were quantified in sera from 35 patients with nonalcoholic steatohepatitis (NASH), 21 patients with chronic hepatitis C (HCV), and 18 healthy controls. Analysis of serum CK18-Asp396 levels showed an increasing trend starting from healthy controls (median: 54.5 U/l), to HCV patients (80.1 U/l), to patients with NASH (144.1 U/l, Kruskall-Wallis test: P<0.001). Post hoc analyses revealed that CK18-Asp396 levels were significantly higher in the NASH patients than in both HCV patients (P=0.008) and healthy controls (P<0.001). Moreover, the levels were significantly higher in patients with HCV than in control individuals (P<0.05). In patients with chronic HCV infection there was a significant positive correlation between serum CK18-Asp396 levels and AST (r=0.442, P<0.05), the ultrasonographic grade of steatosis (r=0.446, P<0.05), and the histological steatosis score (r=0.759, P<0.001). Although subject to future confirmation, these pilot findings seem to indicate that serum levels of caspase-cleaved cytokeratin 18 (CK18-Asp396) are higher in patients with NASH than in those with chronic HCV infection. These data suggest that NASH patients have an increased hepatocyte loss by apoptosis compared with chronic hepatitis C patients.

  3. Can Recent Global Changes Explain the Dramatic Range Contraction of an Endangered Semi-Aquatic Mammal Species in the French Pyrenees?

    Science.gov (United States)

    Charbonnel, Anaïs; Laffaille, Pascal; Biffi, Marjorie; Blanc, Frédéric; Maire, Anthony; Némoz, Mélanie; Sanchez-Perez, José Miguel; Sauvage, Sabine; Buisson, Laëtitia

    2016-01-01

    Species distribution models (SDMs) are the main tool to predict global change impacts on species ranges. Climate change alone is frequently considered, but in freshwater ecosystems, hydrology is a key driver of the ecology of aquatic species. At large scale, hydrology is however rarely accounted for, owing to the lack of detailed stream flow data. In this study, we developed an integrated modelling approach to simulate stream flow using the hydrological Soil and Water Assessment Tool (SWAT). Simulated stream flow was subsequently included as an input variable in SDMs along with topographic, hydrographic, climatic and land-cover descriptors. SDMs were applied to two temporally-distinct surveys of the distribution of the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees: a historical one conducted from 1985 to 1992 and a current one carried out between 2011 and 2013. The model calibrated on historical data was also forecasted onto the current period to assess its ability to describe the distributional change of the Pyrenean desman that has been modelled in the recent years. First, we found that hydrological and climatic variables were the ones influencing the most the distribution of this species for both periods, emphasizing the importance of taking into account hydrology when SDMs are applied to aquatic species. Secondly, our results highlighted a strong range contraction of the Pyrenean desman in the French Pyrenees over the last 25 years. Given that this range contraction was under-estimated when the historical model was forecasted onto current conditions, this finding suggests that other drivers may be interacting with climate, hydrology and land-use changes. Our results imply major concerns for the conservation of this endemic semi-aquatic mammal since changes in climate and hydrology are expected to become more intense in the future.

  4. Can Recent Global Changes Explain the Dramatic Range Contraction of an Endangered Semi-Aquatic Mammal Species in the French Pyrenees?

    Directory of Open Access Journals (Sweden)

    Anaïs Charbonnel

    Full Text Available Species distribution models (SDMs are the main tool to predict global change impacts on species ranges. Climate change alone is frequently considered, but in freshwater ecosystems, hydrology is a key driver of the ecology of aquatic species. At large scale, hydrology is however rarely accounted for, owing to the lack of detailed stream flow data. In this study, we developed an integrated modelling approach to simulate stream flow using the hydrological Soil and Water Assessment Tool (SWAT. Simulated stream flow was subsequently included as an input variable in SDMs along with topographic, hydrographic, climatic and land-cover descriptors. SDMs were applied to two temporally-distinct surveys of the distribution of the endangered Pyrenean desman (Galemys pyrenaicus in the French Pyrenees: a historical one conducted from 1985 to 1992 and a current one carried out between 2011 and 2013. The model calibrated on historical data was also forecasted onto the current period to assess its ability to describe the distributional change of the Pyrenean desman that has been modelled in the recent years. First, we found that hydrological and climatic variables were the ones influencing the most the distribution of this species for both periods, emphasizing the importance of taking into account hydrology when SDMs are applied to aquatic species. Secondly, our results highlighted a strong range contraction of the Pyrenean desman in the French Pyrenees over the last 25 years. Given that this range contraction was under-estimated when the historical model was forecasted onto current conditions, this finding suggests that other drivers may be interacting with climate, hydrology and land-use changes. Our results imply major concerns for the conservation of this endemic semi-aquatic mammal since changes in climate and hydrology are expected to become more intense in the future.

  5. Can Recent Global Changes Explain the Dramatic Range Contraction of an Endangered Semi-Aquatic Mammal Species in the French Pyrenees?

    Science.gov (United States)

    Charbonnel, Anaïs; Laffaille, Pascal; Biffi, Marjorie; Blanc, Frédéric; Maire, Anthony; Némoz, Mélanie; Sanchez-Perez, José Miguel; Sauvage, Sabine

    2016-01-01

    Species distribution models (SDMs) are the main tool to predict global change impacts on species ranges. Climate change alone is frequently considered, but in freshwater ecosystems, hydrology is a key driver of the ecology of aquatic species. At large scale, hydrology is however rarely accounted for, owing to the lack of detailed stream flow data. In this study, we developed an integrated modelling approach to simulate stream flow using the hydrological Soil and Water Assessment Tool (SWAT). Simulated stream flow was subsequently included as an input variable in SDMs along with topographic, hydrographic, climatic and land-cover descriptors. SDMs were applied to two temporally-distinct surveys of the distribution of the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees: a historical one conducted from 1985 to 1992 and a current one carried out between 2011 and 2013. The model calibrated on historical data was also forecasted onto the current period to assess its ability to describe the distributional change of the Pyrenean desman that has been modelled in the recent years. First, we found that hydrological and climatic variables were the ones influencing the most the distribution of this species for both periods, emphasizing the importance of taking into account hydrology when SDMs are applied to aquatic species. Secondly, our results highlighted a strong range contraction of the Pyrenean desman in the French Pyrenees over the last 25 years. Given that this range contraction was under-estimated when the historical model was forecasted onto current conditions, this finding suggests that other drivers may be interacting with climate, hydrology and land-use changes. Our results imply major concerns for the conservation of this endemic semi-aquatic mammal since changes in climate and hydrology are expected to become more intense in the future. PMID:27467269

  6. Precisions on the structure of the Basque Arc (western Pyrenees, Spain): preliminary results from magnetic fabrics from the Biscay Synclinorium

    Science.gov (United States)

    Vegas, Néstor; Aranguren, Aitor; Rodríguez-Méndez, Lidia; Cuevas, Julia; María Tubía, José; Julián Esteban, José

    2017-04-01

    The Mesozoic Basque-Cantabrian basin (western Pyrenees) was inverted during the Alpine Orogeny in late Cretaceous-Eocene times. The central sector of the basin, the Basque Arc is characterized by the existence of large folds (80 km long) that outline an arc. This study focuses on the interpretation of AMS fabrics in rocks from the Biscay Synclinorium, a major fold system of the Basque Arc that verges to the NE, with sub-horizontal, N110˚ E trending axes and axial planes striking to N110˚ E and dipping steeply to the SW (Calvo-Rathert et al., 2007). The aim of this contribution is characterize the deformation around the Biscay Synclinorium. For this reason we combined fieldwork with magnetic fabric analysis of 95 cores in Upper Cretaceous sedimentary rocks of the Biscay Synclinorium. 68 cores come from the Calcareous formation (marls, sandy limestones and limestones of Cenomanian to Campanian age) that makes part of the northeastern limb of the synclinorium and the remaining 27 cores from the Detrital-calcareous flysch (a multilayer sequence with sandy limestones and marls of Maastrichtian age) that crops out in the synclinorium core (Garrote et al., 1991). In the Upper Cretaceous Calcareous formation there is a penetrative cleavage that mainly strikes to N110˚ E dipping 50˚ to 60˚ to the SW (S1). In the Detrital-calcareous flysch of the core, the best-preserved planar structure is the bedding and only locally an axial planar cleavage is observed. Intersection lineations are sub-horizontal and N110˚ E-trending. The measurements of the magnetic susceptibility provide low k values ranging between 99x10-6 and 403x10-6 SI. The anisotropy of the magnetic susceptibility, P, reaches values of 1.213, pointing to the overprinting of tectonic deformation on primary magnetic fabrics of sedimentary origin. The magnetic foliation shows a fairly uniform arrangement that is nearly coincident with the dominant planar structures of the rocks, S0 or S1in the core and the limb

  7. ASP5878, a Novel Inhibitor of FGFR1, 2, 3, and 4, Inhibits the Growth of FGF19-Expressing Hepatocellular Carcinoma.

    Science.gov (United States)

    Futami, Takashi; Okada, Hidetsugu; Kihara, Rumi; Kawase, Tatsuya; Nakayama, Ayako; Suzuki, Tomoyuki; Kameda, Minoru; Shindoh, Nobuaki; Terasaka, Tadashi; Hirano, Masaaki; Kuromitsu, Sadao

    2017-01-01

    Hepatocellular carcinoma is an aggressive cancer with poor prognosis. Fibroblast growth factor 19, a member of the fibroblast growth factor family, is a ligand for fibroblast growth factor receptor 4. Moreover, it plays a crucial role in the progression of hepatocellular carcinoma. ASP5878 is a novel inhibitor of fibroblast growth factor receptors 1, 2, 3, and 4 that is under development. It inhibits fibroblast growth factor receptor 4 kinase activity with an IC 50 of 3.5 nmol/L. ASP5878 potently suppressed the growth of the fibroblast growth factor 19-expressing hepatocellular carcinoma cell lines Hep3B2.1-7, HuH-7, and JHH-7. In the Hep3B2.1-7 cell line, ASP5878 inhibited the phosphorylation of fibroblast growth factor receptor 4 and its downstream signaling molecules as well as induced apoptosis. Oral administration of ASP5878 at 3 mg/kg induced sustained tumor regression in a subcutaneous xenograft mouse model using Hep3B2.1-7. In HuH-7, an orthotopic xenograft mouse model, ASP5878 induced complete tumor regression and dramatically extended the survival of the mice. These results suggest that ASP5878 is a potentially effective therapeutic agent for hepatocellular carcinoma patients with tumors expressing fibroblast growth factor 19. Mol Cancer Ther; 16(1); 68-75. ©2016 AACR. ©2016 American Association for Cancer Research.

  8. One Valley at a Time

    Science.gov (United States)

    2006-08-01

    teaching, outreach, and research in the science and art of joint special operations. JSOU provides education to the men and women of Special Operations... leftover guerrillas. One valley at a time, stability can spread. This persistent spreading of influence into for- mally controlled Taliban areas, one...world. To carry out some of these transactions, Al Qaeda operates in Malaysia, China , the Philippines, and Germany. 10 JSOU Report 06-6 History of the

  9. Structural and functional importance of transmembrane domain 3 (TM3) in the aspartate:alanine antiporter AspT: topology and function of the residues of TM3 and oligomerization of AspT.

    Science.gov (United States)

    Nanatani, Kei; Maloney, Peter C; Abe, Keietsu

    2009-04-01

    AspT, the aspartate:alanine antiporter of Tetragenococcus halophilus, a membrane protein of 543 amino acids with 10 putative transmembrane (TM) helices, is the prototype of the aspartate:alanine exchanger (AAE) family of transporters. Because TM3 (isoleucine 64 to methionine 85) has many amino acid residues that are conserved among members of the AAE family and because TM3 contains two charged residues and four polar residues, it is thought to be located near (or to form part of) the substrate translocation pathway that includes the binding site for the substrates. To elucidate the role of TM3 in the transport process, we carried out cysteine-scanning mutagenesis. The substitutions of tyrosine 75 and serine 84 had the strongest inhibitory effects on transport (initial rates of l-aspartate transport were below 15% of the rate for cysteine-less AspT). Considerable but less-marked effects were observed upon the replacement of methionine 70, phenylalanine 71, glycine 74, arginine 76, serine 83, and methionine 85 (initial rates between 15% and 30% of the rate for cysteine-less AspT). Introduced cysteine residues at the cytoplasmic half of TM3 could be labeled with Oregon green maleimide (OGM), whereas cysteines close to the periplasmic half (residues 64 to 75) were not labeled. These results suggest that TM3 has a hydrophobic core on the periplasmic half and that hydrophilic residues on the cytoplasmic half of TM3 participate in the formation of an aqueous cavity in membranes. Furthermore, the presence of l-aspartate protected the cysteine introduced at glycine 62 against a reaction with OGM. In contrast, l-aspartate stimulated the reactivity of the cysteine introduced at proline 79 with OGM. These results demonstrate that TM3 undergoes l-aspartate-induced conformational alterations. In addition, nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses and a glutaraldehyde cross-linking assay suggest that functional AspT forms homo-oligomers as a

  10. Structural and Functional Importance of Transmembrane Domain 3 (TM3) in the Aspartate:Alanine Antiporter AspT: Topology and Function of the Residues of TM3 and Oligomerization of AspT▿

    Science.gov (United States)

    Nanatani, Kei; Maloney, Peter C.; Abe, Keietsu

    2009-01-01

    AspT, the aspartate:alanine antiporter of Tetragenococcus halophilus, a membrane protein of 543 amino acids with 10 putative transmembrane (TM) helices, is the prototype of the aspartate:alanine exchanger (AAE) family of transporters. Because TM3 (isoleucine 64 to methionine 85) has many amino acid residues that are conserved among members of the AAE family and because TM3 contains two charged residues and four polar residues, it is thought to be located near (or to form part of) the substrate translocation pathway that includes the binding site for the substrates. To elucidate the role of TM3 in the transport process, we carried out cysteine-scanning mutagenesis. The substitutions of tyrosine 75 and serine 84 had the strongest inhibitory effects on transport (initial rates of l-aspartate transport were below 15% of the rate for cysteine-less AspT). Considerable but less-marked effects were observed upon the replacement of methionine 70, phenylalanine 71, glycine 74, arginine 76, serine 83, and methionine 85 (initial rates between 15% and 30% of the rate for cysteine-less AspT). Introduced cysteine residues at the cytoplasmic half of TM3 could be labeled with Oregon green maleimide (OGM), whereas cysteines close to the periplasmic half (residues 64 to 75) were not labeled. These results suggest that TM3 has a hydrophobic core on the periplasmic half and that hydrophilic residues on the cytoplasmic half of TM3 participate in the formation of an aqueous cavity in membranes. Furthermore, the presence of l-aspartate protected the cysteine introduced at glycine 62 against a reaction with OGM. In contrast, l-aspartate stimulated the reactivity of the cysteine introduced at proline 79 with OGM. These results demonstrate that TM3 undergoes l-aspartate-induced conformational alterations. In addition, nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses and a glutaraldehyde cross-linking assay suggest that functional AspT forms homo-oligomers as a

  11. Chloroplast His-to-Asp signal transduction: a potential mechanism for plastid gene regulation in Heterosigma akashiwo (Raphidophyceae

    Directory of Open Access Journals (Sweden)

    Jacobs Michael A

    2007-05-01

    Full Text Available Abstract Background Maintenance of homeostasis requires that an organism perceive selected physical and chemical signals within an informationally dense environment. Functionally, an organism uses a variety of signal transduction arrays to amplify and convert these perceived signals into appropriate gene transcriptional responses. These changes in gene expression serve to modify selective metabolic processes and thus optimize reproductive success. Here we analyze a chloroplast-encoded His-to-Asp signal transduction circuit in the stramenopile Heterosigma akashiwo (Hada Hada ex Y. Hara et Chihara [syn. H. carterae (Hulburt F.J.R. Taylor]. The presence, structure and putative function of this protein pair are discussed in the context of their evolutionary homologues. Results Bioinformatic analysis of the Heterosigma akashiwo chloroplast genome sequence revealed the presence of a single two-component His-to-Asp (designated Tsg1/Trg1 pair in this stramenopile (golden-brown alga. These data represent the first documentation of a His-to-Asp array in stramenopiles and counter previous reports suggesting that such regulatory proteins are lacking in this taxonomic cluster. Comparison of the 43 kDa H. akashiwo Tsg1 with bacterial sensor kinases showed that the algal protein exhibits a moderately maintained PAS motif in the sensor kinase domain as well as highly conserved H, N, G1 and F motifs within the histidine kinase ATP binding site. Molecular modelling of the 27 kDa H. akashiwo Trg1 regulator protein was consistent with a winged helix-turn-helix identity – a class of proteins that is known to impact gene expression at the level of transcription. The occurrence of Trg1 protein in actively growing H. akashiwo cells was verified by Western analysis. The presence of a PhoB-like RNA polymerase loop in Trg1 and its homologues in the red-algal lineage support the hypothesis that Trg1 and its homologues interact with a sigma 70 (σ70 subunit (encoded by

  12. HLA-DPβ1 Asp84-Lys69 antigen-binding signature predicts event-free survival in childhood B-cell precursor acute lymphoblastic leukaemia: results from the MRC UKALL XI childhood ALL trial.

    Science.gov (United States)

    Taylor, G M; Wade, R; Hussain, A; Thompson, P; Hann, I; Gibson, B; Eden, T; Richards, S

    2012-07-01

    We previously reported that children in the UKALL XI ALL trial with HLA-DP 1 and -DP 3 supertypes had significantly worse event-free survival (EFS) than children with other DP supertypes. As DP 1 and DP 3 share two of four key antigen-binding amino-acid polymorphisms (aspartic acid84-lysine69), we asked whether Asp84-Lys69 or Asp84 alone were independent prognostic indicators in childhood acute lymphoblastic leukemia (ALL). We analysed EFS in 798 UKALL XI patients, stratified by Asp84-Lys69 vs non-Asp84-Lys69, for a median follow-up of 12.5 years. Asp84-Lys69 was associated with a significantly worse EFS than non-Asp84-Lys69 (5-year EFS: Asp84-Lys69: 58.8% (95% CI (confidence of interval): 52.7-64.9%); non-Asp84-Lys69: 67.3% (63.4-71.2%); 2P=0.007). Post-relapse EFS was 10% less in Asp84-Lys69 than non-Asp84-Lys69 patients. EFS was significantly worse (P=0.03) and post-relapse EFS marginally worse (P=0.06) in patients with Asp84 compared with Gly84. These results suggest that Asp84-Lys69 predicted adverse EFS in the context of UKALL XI because of Asp84, and may have influenced post-relapse EFS. We speculate that this may be due to the recruitment of Asp84-Lys69-restricted regulatory T cells in the context of this regimen, leading to the re-emergence of residual disease. However, functional and molecular studies of the prognostic value of this and other HLA molecular signatures in other childhood ALL trials are needed.

  13. Medidas estructurales implementadas ante el riesgo de inundación en el núcleo urbano de Aspe (Alicante)

    OpenAIRE

    Sánchez Almodóvar, Esther

    2017-01-01

    El análisis de la evolución urbana del municipio de Aspe centra su interés en identificar las zonas de escorrentía y arterias fluviales que con la evolución urbanística pasan a formar parte del entramado urbano. Estas transformaciones territoriales son el factor clave que explican el riesgo de inundación, ya que las aguas pluviales vierten hacia el núcleo urbano, convirtiendo a las calles en auténticos torrentes. Ante pretéritas riadas por esta causa, se han llevado a cabo medidas estructural...

  14. California's restless giant: the Long Valley Caldera

    Science.gov (United States)

    Hill, David P.; Bailey, Roy A.; Hendley, James W.; Stauffer, Peter H.; Marcaida, Mae

    2014-01-01

    Scientists have monitored geologic unrest in the Long Valley, California, area since 1980. In that year, following a swarm of strong earthquakes, they discovered that the central part of the Long Valley Caldera had begun actively rising. Unrest in the area persists today. The U.S. Geological Survey (USGS) continues to provide the public and civil authorities with current information on the volcanic hazard at Long Valley and is prepared to give timely warnings of any impending eruption.

  15. Structure of N-Terminal Sequence Asp-Ala-Glu-Phe-Arg-His-Asp-Ser of Aβ-Peptide with Phospholipase A2 from Venom of Andaman Cobra Sub-Species Naja naja sagittifera at 2.0 Å Resolution

    Directory of Open Access Journals (Sweden)

    Zeenat Mirza

    2014-03-01

    Full Text Available Alzheimer’s disease (AD is one of the most significant social and health burdens of the present century. Plaques formed by extracellular deposits of amyloid β (Aβ are the prime player of AD’s neuropathology. Studies have implicated the varied role of phospholipase A2 (PLA2 in brain where it contributes to neuronal growth and inflammatory response. Overall contour and chemical nature of the substrate-binding channel in the low molecular weight PLA2s are similar. This study involves the reductionist fragment-based approach to understand the structure adopted by N-terminal fragment of Alzheimer’s Aβ peptide in its complex with PLA2. In the current communication, we report the structure determined by X-ray crystallography of N-terminal sequence Asp-Ala-Glu-Phe-Arg-His-Asp-Ser (DAEFRHDS of Aβ-peptide with a Group I PLA2 purified from venom of Andaman Cobra sub-species Naja naja sagittifera at 2.0 Å resolution (Protein Data Bank (PDB Code: 3JQ5. This is probably the first attempt to structurally establish interaction between amyloid-β peptide fragment and hydrophobic substrate binding site of PLA2 involving H bond and van der Waals interactions. We speculate that higher affinity between Aβ and PLA2 has the therapeutic potential of decreasing the Aβ–Aβ interaction, thereby reducing the amyloid aggregation and plaque formation in AD.

  16. Small martian valleys: Pristine and degraded morphology

    International Nuclear Information System (INIS)

    Baker, V.R.; Partridge, J.B.

    1986-01-01

    The equatorial heavily cratered uplands of Mars are dissected by two classes of small valleys that are intimately associated in compound networks. Pristine valleys with steep valley walls preferentially occupy downstream portions of compound basins. Degraded valleys with eroded walls are laterally more extensive and have higher drainage densities than pristine valleys. Morphometric and crater-counting studies indicate that relatively dense drainage networks were emplaced on Mars during the heavy bombardment about 4.0 b.y. ago. Over a period of approximately 10 8 years, these networks were degraded and subsequently invaded by headwardly extending pristine valleys. The pristine valleys locally reactivated the compound networks, probably through sapping processes dependent upon high water tables. Fluvial activity in the heavily cratered uplands generally ceased approximately 3.8--3.9 b.y. ago, coincident with the rapid decline in cratering rates. The relict compound valleys on Mars are morphometrically distinct from most terrestrial drainage systems. The differences might be caused by a Martian valley formation episode characterized by hyperaridity, by inadequate time for network growth, by very permeable rock types, or by a combination of factors

  17. EPA Region 1 - Valley Depth in Meters

    Science.gov (United States)

    Raster of the Depth in meters of EPA-delimited Valleys in Region 1.Valleys (areas that are lower than their neighbors) were extracted from a Digital Elevation Model (USGS, 30m) by finding the local average elevation, subtracting the actual elevation from the average, and selecting areas where the actual elevation was below the average. The landscape was sampled at seven scales (circles of 1, 2, 4, 7, 11, 16, and 22 km radius) to take into account the diversity of valley shapes and sizes. Areas selected in at least four scales were designated as valleys.

  18. Mengukur Kesiapan Implementasi Customer Relationship Management (CRM Model Application Service Provider (ASP pada Usaha Mikro Kecil Menengah (UMKM di Indonesia

    Directory of Open Access Journals (Sweden)

    Rianto

    2017-04-01

    Full Text Available UMKM menjadi penyumbang utama pendapatan nasional untuk suatu Negara, khususnya Negara berkembang. UMKM tumbuh dan menyerap banyak tenaga kerja dan menjadi roda perekonomian utama. Indonesia menjadi salah satu dari banyak negara, dimana UMKM memberikan banyak kontribusi pendapatan nasional dan penyerapan tenaga kerja. Dalam bisnis, perubahan diperlukan agar tetap hidup dan berkembang, dalam hal ini perubahan bisnis yang bersifat konvensional ke e-bisnis. Salah satunya adalah CRM, CRM telah banyak digunakan oleh perusahaan-perusahaan besar untuk mengembangkan bisnis mereka. Implementasi CRM membutuhkan modal yang besar, khususnya aplikasi CRM model tradisional. Dibandingkan dengan Model ASP, CRM model ini sangat sesuai untuk UMKM khususnya di Indonesia karena dari segi keuangan dapat dijangkau oleh UMKM di Indonesia. Pada penelitian ini sebanyak 30 UMKM tingkat menengah digunakan sebagai koresponden dan digunakan untuk mengukur kesiapan UMKM di Indonesia menggunakan teknologi CRM dengan model ASP. Dimana pada hasil penelitian mengenai kegiatan yang berhubungan dengan CRM dilihat dari dimensi intelektual, sosial dan teknologi masih terlihat sangat rendah.

  19. Bacillus thuringiensis Crystal Protein Cry6Aa Triggers Caenorhabditis elegans Necrosis Pathway Mediated by Aspartic Protease (ASP-1.

    Directory of Open Access Journals (Sweden)

    Fengjuan Zhang

    2016-01-01

    Full Text Available Cell death plays an important role in host-pathogen interactions. Crystal proteins (toxins are essential components of Bacillus thuringiensis (Bt biological pesticides because of their specific toxicity against insects and nematodes. However, the mode of action by which crystal toxins to induce cell death is not completely understood. Here we show that crystal toxin triggers cell death by necrosis signaling pathway using crystal toxin Cry6Aa-Caenorhabditis elegans toxin-host interaction system, which involves an increase in concentrations of cytoplasmic calcium, lysosomal lyses, uptake of propidium iodide, and burst of death fluorescence. We find that a deficiency in the necrosis pathway confers tolerance to Cry6Aa toxin. Intriguingly, the necrosis pathway is specifically triggered by Cry6Aa, not by Cry5Ba, whose amino acid sequence is different from that of Cry6Aa. Furthermore, Cry6Aa-induced necrosis pathway requires aspartic protease (ASP-1. In addition, ASP-1 protects Cry6Aa from over-degradation in C. elegans. This is the first demonstration that deficiency in necrosis pathway confers tolerance to Bt crystal protein, and that Cry6A triggers necrosis represents a newly added necrosis paradigm in the C. elegans. Understanding this model could lead to new strategies for nematode control.

  20. Roles of Asp179 and Glu270 in ADP-Ribosylation of Actin by Clostridium perfringens Iota Toxin.

    Directory of Open Access Journals (Sweden)

    Alexander Belyy

    Full Text Available Clostridium perfringens iota toxin is a binary toxin composed of the enzymatically active component Ia and receptor binding component Ib. Ia is an ADP-ribosyltransferase, which modifies Arg177 of actin. The previously determined crystal structure of the actin-Ia complex suggested involvement of Asp179 of actin in the ADP-ribosylation reaction. To gain more insights into the structural requirements of actin to serve as a substrate for toxin-catalyzed ADP-ribosylation, we engineered Saccharomyces cerevisiae strains, in which wild type actin was replaced by actin variants with substitutions in residues located on the Ia-actin interface. Expression of the actin mutant Arg177Lys resulted in complete resistance towards Ia. Actin mutation of Asp179 did not change Ia-induced ADP-ribosylation and growth inhibition of S. cerevisiae. By contrast, substitution of Glu270 of actin inhibited the toxic action of Ia and the ADP-ribosylation of actin. In vitro transcribed/translated human β-actin confirmed the crucial role of Glu270 in ADP-ribosylation of actin by Ia.

  1. Radwaste challenge at Beaver Valley

    International Nuclear Information System (INIS)

    Anon.

    1984-01-01

    Duquesne Light Company met the problem of accumulating low-level radioactive waste at its Beaver Valley nuclear plant with an aggressive program to reduce the quantity of contaminated material and demonstrate that the plant was improving its radiological protection. There was also an economic incentive to reduce low-level wastes. The imaginative campaign involved workers in the reduction effort through training and the adoption of practical approaches to reducing the amount of material exposed to radiation that include sorting trash by radiation level and a compacting system. 4 figures

  2. Annual and seasonal analysis of temperature and precipitation in Andorra (Pyrenees) from 1934 to 2008: quality check, homogenization and trends

    Science.gov (United States)

    Esteban, Pere; Prohom, Marc; Aguilar, Enric; Mestre, Olivier

    2010-05-01

    ://www.meteo.cat) - Spagnoli B, Planton S, Mestre O, Déqué M, Moisselin, JM (2002). Detecting climate change at a regional scale: the case of France. Geophys. Res. Lett., 29, n.10, pp. 91-94. - Maris M, Giraud G, Durand Y, Navarre JP, Mérindol L. 2009. Results of 50 years of climate reanalysis in the French Pyrenees (1958-2008) using SAFRAN and CROCUS models. International Snow Science Workshop, Davos 2009, Proceedings.

  3. Glutathione S-transferase omega-2 polymorphism Asn142Asp modifies the risk of age-related cataract in smokers and subjects exposed to ultraviolet irradiation.

    Science.gov (United States)

    Stamenkovic, Miroslav; Radic, Tanja; Stefanovic, Ivan; Coric, Vesna; Sencanic, Ivan; Pljesa-Ercegovac, Marija; Matic, Marija; Jaksic, Vesna; Simic, Tatjana; Savic-Radojevic, Ana

    2014-04-01

    Glutathione S-transferase omega-1 and 2 have a unique range of enzymatic activities, including the regeneration of ascorbate by their dehydroascorbate reductase activities. Because these enzymes could have a protective role from oxidative damage in the lens, the question of whether the two coding glutathione S-transferase omega polymorphisms confer the risk of age-related cataract was addressed. rs4925 (Ala140Asp) of glutathione S-transferase omega-1 and rs156697 (Asn142Asp) of glutathione S-transferase omega-2 polymorphisms in 100 patients with age-related cataract and 130 controls were assessed. Presence of one mutant GSTO1*Asp or GSTO2*Asp allele did not contribute independently towards the risk of cataract; however, homozygous carriers of GSTO1*Asp/GSTO2*Asp haplotype demonstrated 3.42-fold enhanced risk of cataract development (95% confidence interval = 0.84-13.93; P = 0.086). When GSTO genotype was analysed in association with smoking or professional exposure to ultraviolet irradiation, carriers of at least one mutant GSTO2*Asp allele had increased risk of cataract development in comparison with individuals with wild-type GSTO2*Asn/Asn with no history of smoking or ultraviolet exposure (odds ratio = 6.89, 95% confidence interval = 1.81-16.21, P = 0.005; odds ratio = 4.10, 95% confidence interval = 1.23-13.74, P = 0.022, respectively). Regarding the distribution of particular glutathione S-transferase omega genotype and cataract type, the highest frequency of mutant GSTO2*Asp allele was found in patients with nuclear cataract. The results indicate that mutant GSTO2*Asp genotype is associated with increased risk of age-related cataract in smokers and ultraviolet-exposed subjects, suggesting a role of inefficient ascorbate regeneration in cataract development. © 2013 Royal Australian and New Zealand College of Ophthalmologists.

  4. The adjuvanticity of an O. volvulus-derived rOv-ASP-1 protein in mice using sequential vaccinations and in non-human primates.

    Science.gov (United States)

    Wang, Jing; Tricoche, Nancy; Du, Lanying; Hunter, Meredith; Zhan, Bin; Goud, Gaddam; Didier, Elizabeth S; Liu, Jing; Lu, Lu; Marx, Preston A; Jiang, Shibo; Lustigman, Sara

    2012-01-01

    Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the Onchocerca volvulus recombinant protein rOv-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6. However, because it is a protein, the rOv-ASP-1 adjuvant may also induce anti-self-antibodies. Therefore, it was important to verify that the host responses to self will not affect the adjuvanticity of rOv-ASP-1 when it is used in subsequent vaccinations with the same or different vaccine antigens. In this study, we have established rOv-ASP-1's adjuvanticity in mice during the course of two sequential vaccinations using two vaccine model systems: the receptor-binding domain (RBD) of SARS-CoV spike protein and a commercial influenza virus hemagglutinin (HA) vaccine comprised of three virus strains. Moreover, the adjuvanticity of rOv-ASP-1 was retained with an efficacy similar to that obtained when it was used for a first vaccination, even though a high level of anti-rOv-ASP-1 antibodies was present in the sera of mice before the administration of the second vaccine. To further demonstrate its utility as an adjuvant for human use, we also immunized non-human primates (NHPs) with RBD plus rOv-ASP-1 and showed that rOv-ASP-1 could induce high titres of functional and protective anti-RBD antibody responses in NHPs. Notably, the rOv-ASP-1 adjuvant did not induce high titer antibodies against self in NHPs. Thus, the present study provided a sound scientific foundation for future strategies in the development of this novel protein adjuvant.

  5. L-Asp is a useful tool in the purification of the ionotropic glutamate receptor A2 ligand-binding domain

    DEFF Research Database (Denmark)

    Krintel, Christian; Frydenvang, Karla; Ceravalls de Rabassa, Anna

    2014-01-01

    In purification of the ionotropic glutamate receptor A2 (GluA2) ligand-binding domain (LBD), L-Glu supplemented buffers have previously been used for protein stabilization during the procedure. This sometimes hampers structural studies of low affinity ligands because L-Glu is difficult to displace...... crystallized as a mixed dimer with L-Glu present in one subunit while neither L-Asp nor L-Glu were found in the other subunit. Thus, residual L-Glu is still present from the expression. On the other hand, only L-Asp was found at the binding site when using 50 mM or 250 mM L-Asp for crystallization. The binding...... mode observed for L-Asp at the GluA2 LBD is very similar to that described for L-Glu. Taken together, we have shown that L-Asp can be used instead of L-Glu for ligand-dependent stabilization of the GluA2 LBD during purification. This will enable structural studies of low affinity ligands for lead...

  6. Valley dependent transport in graphene L junction

    Science.gov (United States)

    Chan, K. S.

    2018-05-01

    We studied the valley dependent transport in graphene L junctions connecting an armchair lead and a zigzag lead. The junction can be used in valleytronic devices and circuits. Electrons injected from the armchair lead into the junction is not valley polarized, but they can become valley polarized in the zigzag lead. There are Fermi energies, where the current in the zigzag lead is highly valley polarized and the junction is an efficient generator of valley polarized current. The features of the valley polarized current depend sensitively on the widths of the two leads, as well as the number of dimers in the armchair lead, because this number has a sensitive effect on the band structure of the armchair lead. When an external potential is applied to the junction, the energy range with high valley polarization is enlarged enhancing its function as a generator of highly valley polarized current. The scaling behavior found in other graphene devices is also found in L junctions, which means that the results presented here can be extended to junctions with larger dimensions after appropriate scaling of the energy.

  7. Sustainable agricultural development in inland valleys

    NARCIS (Netherlands)

    Zwart, S.J.

    2018-01-01

    The inland valley in Africa are common landscapes that have favorable conditions for agricultural production. Compared to the surrounding uplands they are characterized by a relatively high and secure water availability and high soil fertility levels. Inland valleys thus have a high agricultural

  8. Rift Valley fever outbreak, southern Mauritania, 2012.

    Science.gov (United States)

    Sow, Abdourahmane; Faye, Ousmane; Ba, Yamar; Ba, Hampathé; Diallo, Diawo; Faye, Oumar; Loucoubar, Cheikh; Boushab, Mohamed; Barry, Yahya; Diallo, Mawlouth; Sall, Amadou Alpha

    2014-02-01

    After a period of heavy rainfall, an outbreak of Rift Valley fever occurred in southern Mauritania during September-November 2012. A total of 41 human cases were confirmed, including 13 deaths, and 12 Rift Valley fever virus strains were isolated. Moudjeria and Temchecket Departments were the most affected areas.

  9. 27 CFR 9.154 - Chiles Valley.

    Science.gov (United States)

    2010-04-01

    ... viticultural area are four 1:24,000 Scale U.S.G.S. topography maps. They are titled: (1) St. Helena, CA 1960... Valley viticultural area, using landmarks and points of reference found on appropriate U.S.G.S. maps..., with a county road known locally as Chiles and Pope Valley Road; (5) Then in a southwesterly direction...

  10. Beaver assisted river valley formation

    Science.gov (United States)

    Westbrook, Cherie J.; Cooper, D.J.; Baker, B.W.

    2011-01-01

    We examined how beaver dams affect key ecosystem processes, including pattern and process of sediment deposition, the composition and spatial pattern of vegetation, and nutrient loading and processing. We provide new evidence for the formation of heterogeneous beaver meadows on riverine system floodplains and terraces where dynamic flows are capable of breaching in-channel beaver dams. Our data show a 1.7-m high beaver dam triggered overbank flooding that drowned vegetation in areas deeply flooded, deposited nutrient-rich sediment in a spatially heterogeneous pattern on the floodplain and terrace, and scoured soils in other areas. The site quickly de-watered following the dam breach by high stream flows, protecting the deposited sediment from future re-mobilization by overbank floods. Bare sediment either exposed by scouring or deposited by the beaver flood was quickly colonized by a spatially heterogeneous plant community, forming a beaver meadow. Many willow and some aspen seedlings established in the more heavily disturbed areas, suggesting the site may succeed to a willow carr plant community suitable for future beaver re-occupation. We expand existing theory beyond the beaver pond to include terraces within valleys. This more fully explains how beavers can help drive the formation of alluvial valleys and their complex vegetation patterns as was first postulated by Ruedemann and Schoonmaker in 1938. ?? 2010 John Wiley & Sons, Ltd.

  11. "The Pyrenees are not hollow": the mountain as a boundary object "Os Pirineus não são ecos": a montanha como objeto de fronteira

    Directory of Open Access Journals (Sweden)

    Jérôme Lamy

    2009-09-01

    Full Text Available In Toulouse, around 1850, a controversy about the structure of the Pyrenees pitted observatory director Frederic Petit against geology professor Alexandre Leymerie. The object of the debate was an assumption formulated by Petit: that the inside of the Pyrenees was practically hollow. This proposal was based on work that Petit initiated in order to determine the latitude of Toulouse. The debates, which took place within the Toulouse Academy of Science and also in local newspapers, illustrate the organization of disciplinary spaces in the nineteenth century. Petit defended his research method based on calculation; the geologist's perspective was from the field. The emergence of the less mathematical science of geology came up against nineteenth-century astronomical practices, centered on calculation. Dissected by calculation or by visual observation, the mountain was an object of controversy from the perspective of distinct scientific practices.Em Toulouse, por volta de 1850, uma controvérsia sobre a estrutura dos Pirineus colocou o diretor do Observatório, Frederic Petit, contra o professor de geologia Alexandre Leymerie. O motivo do debate foi a hipótese formulada por Petit: o interior dos Pirineus era praticamente oco. A proposição baseava-se no trabalho que Petit iniciara para determinar a latitude de Toulouse. Os debates, que tiveram lugar na Academia de Ciências de Toulouse e também em periódicos locais, ilustram a organização dos espaços disciplinares no século XIX. Petit defendia seu método de pesquisa baseado em cálculos; a perspectiva do geólogo provinha do campo. A emergência da ciência geológica, menos matemática, vinha de encontro às práticas da astronomia daquele século. Analisada por cálculos ou pela observação visual, a montanha foi um objeto de controvérsia entre diferentes práticas científicas.

  12. Effects of functionalization of PLGA-[Asp-PEG]n copolymer surfaces with Arg-Gly-Asp peptides, hydroxyapatite nanoparticles, and BMP-2-derived peptides on cell behavior in vitro.

    Science.gov (United States)

    Pan, Haitao; Zheng, Qixin; Yang, Shuhua; Guo, Xiaodong

    2014-12-01

    Functionalization of polymer surfaces has been recognized as a valuable tool to improve their properties that significantly influence cellular behaviors, such as adhesion, proliferation, migration, and differentiation. In stem cell-mediated bone tissue engineering, surface multifunctionalization of polymeric scaffolds with cell-adhesive, osteoconductive, and osteoinductive biomolecules is a critical strategy to improve such properties. However, the traditional surface modification techniques such as physical deposition/adsorption, chemical modification, grafting, and plasma techniques have great limitations for immobilization of multiple bioactive molecules due to multistep procedures. Recently, a universal technique based on mussel-inspired self-polymerization of dopamine is developed for multifunctional coatings in a simple way. In our study, we used this newly developed technique to incorporate three biomolecules, cell adhesion-promoting (K)16 GRGDSPC peptides, osteoconductive hydroxyapatite (HAp) nanoparticles, and osteoinductive bone morphogenetic protein-2-derived P24 peptides, to functionalize poly(lactide-co-glycolide) (PLGA)-[Asp-PEG]n scaffolds, and the effects on biological behaviors of co-cultured rabbit-derived bone marrow stromal cells in vitro were investigated. The results showed (K)16 GRGDSPC, HAp, and P24 could be immobilized onto the scaffolds through predeposition of polydopamine (pDA) ad-layer, and the surface-modified scaffolds were noncytotoxic as well as the virgin scaffold. The pDA-assisted codeposition of (K)16 GRGDSPC, HAp, and P24 on the scaffold surfaces significantly promoted cell adhesion, proliferation, osteodifferentiation, and mineralization in vitro with synergistic effects. Taken together, the functionalized PLGA-[Asp-PEG]n polymeric scaffolds achieved significantly elevated affinity, osteoconductive and osteoinductive ability, and may be a potentially promising bone graft substitute for bone repair. © 2014 Wiley Periodicals

  13. Hyperalgesia induced by Asp49 and Lys49 phospholipases A2 from Bothrops asper snake venom: pharmacological mediation and molecular determinants.

    Science.gov (United States)

    Chacur, M; Longo, I; Picolo, G; Gutiérrez, J M; Lomonte, B; Guerra, J L; Teixeira, C F P; Cury, Y

    2003-05-01

    The ability of Lys49 and Asp49 phospholipases A(2) (PLA(2)), from Bothrops asper snake venom, to cause hyperalgesia was investigated in rats, using the paw pressure test. Intraplantar injection of both toxins (5-20 micro g/paw) caused hyperalgesia, which peaked 1h after injections. Incubation of both proteins with heparin, prior to their injection, partially reduced this response. Chemical modification of Asp49 PLA(2) with p-bromophenacyl bromide (p-BPB), which abrogates its PLA(2) activity, also abolished hyperalgesia. Intraplantar injection of a synthetic peptide corresponding to the C-terminal sequence 115-129 of Lys49 PLA(2), caused hyperalgesia of similar time course, but varying magnitude, than that induced by the native protein. In contrast, a homologous peptide derived from the Asp49 PLA(2) did not show any nociceptive effect. Hyperalgesia induced by both PLA(2)s was blocked by the histamine and serotonin receptor antagonists promethazine and methysergide, respectively, by the bradykinin B(2) receptor antagonist HOE 140 and by antibodies to tumor necrosis factor alfa (TNFalpha) and interleukin 1 (IL-1). Pretreatment with guanethidine, atenolol, prazosin and yohimbine, inhibitors of sympathomimetic amines, or with indomethacin, inhibitor of the cyclo-oxygenase pathway, reduced Lys49 PLA(2)-induced hyperalgesia without interfering with the nociceptive activity of Asp49 PLA(2). The hyperalgesic response to both myotoxins was not modified by pretreatment with celecoxib, an inhibitor of the cyclo-oxygenase type II, by zileuton, an inhibitor of the lipoxygenase pathway or by N(g)-methyl-L-arginine (LNMMA), an inhibitor of nitric oxide synthase. These results suggest that Asp49 and Lys49 PLA(2)s are important hyperalgesic components of B. asper venom, and that Lys49 and Asp49 PLA(2)s exert their algogenic actions through different molecular mechanisms.

  14. A comparative study of occurrence and fate of endocrine disruptors: diethyl phthalate and dibutyl phthalate in ASP- and SBR-based wastewater treatment plants.

    Science.gov (United States)

    Saini, Gita; Pant, Shalini; Singh, Shri Om; Kazmi, A A; Alam, Tanveer

    2016-11-01

    Phthalates are endocrine-disrupting chemicals which affect endocrine system by bio-accumulation in aquatic organisms and produce adverse health effects in aquatic organisms as well as human beings, when come in contact. Present study focuses on occurrence and removal of two phthalates: diethylphthalate (DEP) and dibutylphthalate (DBP) in two full-scale wastewater treatment plants (WWTPs) i.e. sewage treatment plants (STPs) based on well-adopted technologies, activated sludge process (ASP) and sequencing batch reactor (SBR).Gas chromatography-mass spectrometry (GC-MS) analysis was performed for both wastewater and sludge sample for determination and identification of the concentration of these compounds in both STPs by monitoring the STPs for 9 months. It was observed that the concentration of DEP was less than DBP in the influent of ASP and SBR. Average concentrations of DEP and DBP in sludge sample of ASP were found to be 2.15 and 2.08 ng/g, whereas in SBR plant, these values were observed as 1.71 and 2.01 ng/g, respectively. Concerning the removal efficiency of DEP, SBR and ASP plants were found effective with removal efficiency of 91.51 and 91.03 %, respectively. However, in the case of DBP, SBR showed lower removal efficiency (85.42 %) as compared to ASP (92.67 %). Comparative study of both plants proposed that in ASP plant, DBP reduction was higher than the SBR. Fourier transformation infrared (FTIR) analysis also confirmed the same result of sludge analysis for both STPs. Sludge disposal studied with scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and thermo-gravimetric analysis (TGA) techniques confirmed that sludge of both STPs have high calorific value and can be used as fuel to make fuel-briquettes and bottom ash to make firebricks.

  15. A common variant of endothelial nitric oxide synthase (Glu298Asp) is associated with collateral development in patients with chronic coronary occlusions

    International Nuclear Information System (INIS)

    Lamblin, Nicolas; Cuilleret, François J; Helbecque, Nicole; Dallongeville, Jean; Lablanche, Jean-Marc; Amouyel, Philippe; Bauters, Christophe; Van Belle, Eric

    2005-01-01

    Experimental studies support an important role for endothelial nitric oxide synthase (eNOS) in the regulation of angiogenesis. In humans, a common polymorphism exists in the eNOS gene that results in the conversion of glutamate to aspartate for codon 298. In vitro and in vivo studies have suggested a decreased NOS activity in patients with the Asp 298 variant. We hypothesized that a genetic-mediated decreased eNOS activity may limit collateral development in patients with chronic coronary occlusions. We selected 291 consecutive patients who underwent coronary angiography and who had at least one chronic (>15 days) total coronary occlusion. Collateral development was graded angiographically using two different methods: the collateral flow grade and the recipient filling grade. Genomic DNA was extracted from white blood cells and genotyping was performed using previously published techniques. Collateral development was lower in patients carrying the Asp 298 variant than in Glu-Glu homozygotes (collateral flow grade: 2.64 ± 0.08 and 2.89 ± 0.08, respectively, p = 0.04; recipient filling grade: 3.00 ± 0.08 and 3.24 ± 0.07, respectively, p = 0.04). By multivariable analysis, three variables were independently associated with the collateral flow grade: female gender, smoking, and the Asp 298 variant (p = 0.03) while the Asp 298 variant was the sole variable independently associated with the recipient filling grade (p = 0.03). Collateral development is lower in patients with the Asp 298 variant. This may be explained by the decreased NOS activity in patients with the Asp 298 variant. Further studies will have to determine whether increasing eNOS activity in humans is associated with coronary collateral development

  16. Microbial expression of proteins containing long repetitive Arg-Gly-Asp cell adhesive motifs created by overlap elongation PCR

    International Nuclear Information System (INIS)

    Kurihara, Hiroyuki; Shinkai, Masashige; Nagamune, Teruyuki

    2004-01-01

    We developed a novel method for creating repetitive DNA libraries using overlap elongation PCR, and prepared a DNA library encoding repetitive Arg-Gly-Asp (RGD) cell adhesive motifs. We obtained various length DNAs encoding repetitive RGD from a short monomer DNA (18 bp) after a thermal cyclic reaction without a DNA template for amplification, and isolated DNAs encoding 2, 21, and 43 repeats of the RGD motif. We cloned these DNAs into a protein expression vector and overexpressed them as thioredoxin fusion proteins: RGD2, RGD21, and RGD43, respectively. The solubility of RGD43 in water was low and it formed a fibrous precipitate in water. Scanning electron microscopy revealed that RGD43 formed a branched 3D-network structure in the solid state. To evaluate the function of the cell adhesive motifs in RGD43, mouse fibroblast cells were cultivated on the RGD43 scaffold. The fibroblast cells adhered to the RGD43 scaffold and extended long filopodia

  17. Peripherally applied synthetic peptide isoAsp7-Aβ(1-42) triggers cerebral β-amyloidosis.

    Science.gov (United States)

    Kozin, S A; Cheglakov, I B; Ovsepyan, A A; Telegin, G B; Tsvetkov, P O; Lisitsa, A V; Makarov, A A

    2013-10-01

    Intracerebral and intraperitoneal inoculation with β-amyloid-rich brain extracts originating from patients with Alzheimer's disease as well as intracerebral injection of aggregates composed of synthetic Aβ can induce cerebral β-amyloidosis, and associated cognitive dysfunctions in susceptible animal hosts. We have found that repetitive intravenous administration of 100 μg of synthetic peptide corresponding to isoAsp7-containing Aβ(1-42), an abundant age-dependent Aβ isoform present both in the pathological brain and in synthetic Aβ preparations, robustly accelerates formation of classic dense-core congophilic amyloid plaques in the brain of β-amyloid precursor protein transgenic mice. Our findings indicate this peptide as an inductive agent of cerebral β-amyloidosis in vivo.

  18. Synthesis of a precursor dipeptide of RGDS (Arg-Gly-Asp-Ser) catalysed by the industrial protease alcalase.

    Science.gov (United States)

    Hou, Rui-Zhen; Yang, Yan; Li, Gang; Huang, Yi-Bing; Wang, Hua; Liu, Yun-Jia; Xu, Li; Zhang, Xue-Zhong

    2006-05-01

    Synthesis of Bz-Arg-Gly-NH(2) (N-benzoylargininylglycinamide) [a precursor dipeptide of RGDS (Arg-Gly-Asp-Ser)] catalysed by protease in water/organic co-solvent systems was studied. Starting substrates were N-benzoyl-L-arginine ethyl ester hydrochloride (acyl donor) and glycinamide (nucleophile). Acetonitrile was selected as the organic solvent. Alcalase, an industrial alkaline protease, was applied to the synthesis of the target dipeptide. The conditions of the synthesis reaction were optimized by examining the effects of several factors, including water content, temperature, pH, molar ratio of the substrates and reaction time, on the yield of Bz-Arg-Gly-NH(2). The optimum conditions were established to be pH 10.0, 45 degrees C, in acetonitrile/0.1 M Na(2)CO(3)/NaHCO(3) buffer system (90:10, v/v) for 1 h with a dipeptide yield of 82.9%.

  19. Medusa: una aplicación de la integración entre PDF y ASP:NET®

    Directory of Open Access Journals (Sweden)

    CARLOS M. ZAPATA J.

    2005-01-01

    Full Text Available El formato de Documentos Portátiles (PDF por sus siglas en inglés se ha venido consolidando en el mundo como un estándar para la transmisión de documentos vía Web, especialmente reconocidos como sólo lectura. Sin embargo, poco se han trabajado las capacidades interactivas de dicha tecnología, en particular para la captura y transmisión de datos a un servidor. En este artículo se presenta MEDUSA, un software basado en tecnología PDF que interactúa con ASP® y ASP.NET® para el manejo de una plataforma de compras vía Web.

  20. Datación mediante racemización del ácido aspártico en dentina humana

    OpenAIRE

    Cristopher Barriga Salazar; César Rivera Martínez; Iván Suazo Galdames

    2012-01-01

    La determinación de la edad al momento de la muerte sobre la base de la racemización del ácido aspártico (AAR) en la dentina humana se ha aplicado con éxito en odontología forense desde hace varios años. El uso de este procedimiento en la dentina proporciona una solución sencilla y rentable, cuyo método puede lograr una precisión de más menos 3 años. Actualmente esta determinación no se encuentra estandarizada, inconsistencia que se debe resolver si se pretende aplicar con éxito la técnica pa...

  1. Endothelial nitric oxide synthase gene polymorphism (Glu298Asp and development of pre-eclampsia: a case-control study and a meta-analysis

    Directory of Open Access Journals (Sweden)

    Sahemey Manpreet K

    2006-03-01

    Full Text Available Abstract Background Pre-eclampsia is thought to have an important genetic component. Recently, pre-eclampsia has been associated in some studies with carriage of a common eNOS gene Glu298Asp polymorphism, a variant that leads to the replacement of glutamic acid by aspartic acid at codon 298. Method Healthy women with singleton pregnancies were recruited from 7 district general hospitals in London, UK. Women at high risk of pre-eclampsia were screened by uterine artery Doppler velocimetry at 22–24 weeks of gestation and maternal blood was obtained to genotype the eNOS Glu298Asp polymorphism. Odds ratios (OR and 95%CI, using logistic regression methods, were obtained to evaluate the association between the Glu298Asp polymorphism and pre-eclampsia. A meta-analysis was then undertaken of all published studies up to November 2005 examining the association of eNOS Glu298Asp genotype and pre-eclampsia. Results 89 women with pre-eclampsia and 349 controls were included in the new study. The Glu298Asp polymorphism in a recessive model was not significantly associated with pre-eclampsia (adjusted-OR: 0.83 [95%CI: 0.30–2.25]; p = 0.7. In the meta-analysis, under a recessive genetic model (1129 cases & 2384 controls women homozygous for the Asp298 allele were not at significantly increased risk of pre-eclampsia (OR: 1.28 [95%CI: 0.76–2.16]; p = 0.34. A dominant model (1334 cases & 2894 controls was associated with no increase of risk of pre-eclampsia for women carriers of the Asp298 allele (OR: 1.12 [95%CI: 0.84–1.49]; p = 0.42. Conclusion From the data currently available, the eNOS Glu298Asp polymorphism is not associated with a significant increased risk of pre-eclampsia. However, published studies have been underpowered, much larger studies are needed to confirm or refute a realistic genotypic risk of disease, but which might contribute to many cases of pre-eclampsia in the population.

  2. Photosensitive Epilepsy In Kashmir Valley

    Directory of Open Access Journals (Sweden)

    Saleem S M

    2003-01-01

    Full Text Available A random population of 618 people with epilepsy hailing from different parts of Kashmir valley was screened for photosensitivity both clinically and on a standard protocol of intermittent photic stimulation (IPS provoked EEG recordings. Six (0.9% patients with a mean age of 15+6.57 years were found to be photosensitive; five had generalized and one had absence seizures. The baseline EEG in all patients showed generalized epileptiform discharges. On IPS, similar EEG findings were obtained with a narrow range of stimulus frequency i.e. 7-12 cycles/sec. There appears to be a low prevalence of photo-sensitivity in our epileptic population, possibly related to genetic factors.

  3. Novel Bacterial Topoisomerase Inhibitors Exploit Asp83 and the Intrinsic Flexibility of the DNA Gyrase Binding Site

    Directory of Open Access Journals (Sweden)

    Sebastian Franco-Ulloa

    2018-02-01

    Full Text Available DNA gyrases are enzymes that control the topology of DNA in bacteria cells. This is a vital function for bacteria. For this reason, DNA gyrases are targeted by widely used antibiotics such as quinolones. Recently, structural and biochemical investigations identified a new class of DNA gyrase inhibitors called NBTIs (i.e., novel bacterial topoisomerase inhibitors. NBTIs are particularly promising because they are active against multi-drug resistant bacteria, an alarming clinical issue. Structural data recently demonstrated that these NBTIs bind tightly to a newly identified pocket at the dimer interface of the DNA–protein complex. In the present study, we used molecular dynamics (MD simulations and docking calculations to shed new light on the binding of NBTIs to this site. Interestingly, our MD simulations demonstrate the intrinsic flexibility of this binding site, which allows the pocket to adapt its conformation and form optimal interactions with the ligand. In particular, we examined two ligands, AM8085 and AM8191, which induced a repositioning of a key aspartate (Asp83B, whose side chain can rotate within the binding site. The conformational rearrangement of Asp83B allows the formation of a newly identified H-bond interaction with an NH on the bound NBTI, which seems important for the binding of NBTIs having such functionality. We validated these findings through docking calculations using an extended set of cognate oxabicyclooctane-linked NBTIs derivatives (~150, in total, screened against multiple target conformations. The newly identified H-bond interaction significantly improves the docking enrichment. These insights could be helpful for future virtual screening campaigns against DNA gyrase.

  4. Prevalence of endothelial nitric oxide synthase (eNOS) gene exon 7 Glu298Asp variant in North Eastern India

    Science.gov (United States)

    Shankarishan, Priyanka; Borah, Prasanta Kumar; Ahmed, Giasuddin; Mahanta, Jagadish

    2011-01-01

    Background & objectives Endothelial nitric oxide is a potent vasodilator and impairment of its generation brought about by gene polymorphism is considered a major predictor for several diseases. A single nucleotide polymorphism G894T within exon 7 of endothelial nitric oxide synthase (eNOS-7) gene, resulting in a replacement of glutamic acid by aspartic acid, has been studied as a putative candidate gene for cardiovascular diseases. The pattern of eNOS-7 Glu298Asp variant in the Indian population is poorly known. The present study was planned to determine the prevalence of the variant of this gene among tea garden community in Assam, North-East India with high prevalence of hypertension. Methods Study participants of both sex aged ≥18 yr were recruited randomly from temporary field clinics established in tea gardens of Dibrugarh, Assam. Genomic DNA was extracted from 409 subjects by the conventional phenol-chloroform method. The prevalence of the eNOS exon 7 Glu298Asp variant was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results The study population was in Hardy-Weinberg Equilibrium. The frequency of the eNOS GG, GT and TT genotypes was found to be 75, 22 and 3 per cent respectively and did not show any significant difference in gender wise analysis. Interpretation & conclusions Our results showed that the prevalence of the homozygous GG genotype was high (75%) and the rare mutant genotype (homozygous, TT) was 3 per cent in a population at risk with cardiovascular disease. Such population-based data on various polymorphisms can ultimately be exploited in pharmacogenomics. PMID:21623032

  5. A high-order 3-D spectral-element method for the forward modelling and inversion of gravimetric data—Application to the western Pyrenees

    Science.gov (United States)

    Martin, Roland; Chevrot, Sébastien; Komatitsch, Dimitri; Seoane, Lucia; Spangenberg, Hannah; Wang, Yi; Dufréchou, Grégory; Bonvalot, Sylvain; Bruinsma, Sean

    2017-04-01

    We image the internal density structure of the Pyrenees by inverting gravity data using an a priori density model derived by scaling a Vp model obtained by full waveform inversion of teleseismic P-waves. Gravity anomalies are computed via a 3-D high-order finite-element integration in the same high-order spectral-element grid as the one used to solve the wave equation and thus to obtain the velocity model. The curvature of the Earth and surface topography are taken into account in order to obtain a density model as accurate as possible. The method is validated through comparisons with exact semi-analytical solutions. We show that the spectral-element method drastically accelerates the computations when compared to other more classical methods. Different scaling relations between compressional velocity and density are tested, and the Nafe-Drake relation is the one that leads to the best agreement between computed and observed gravity anomalies. Gravity data inversion is then performed and the results allow us to put more constraints on the density structure of the shallow crust and on the deep architecture of the mountain range.

  6. Geology and taphonomy of the L'Espinau dinosaur bonebed, a singular lagoonal site from the Maastrichtian of South-Central Pyrenees

    Science.gov (United States)

    Fondevilla, V.; Vicente, A.; Battista, F.; Sellés, A. G.; Dinarès-Turell, J.; Martín-Closas, C.; Anadón, P.; Vila, B.; Razzolini, N. L.; Galobart, À.; Oms, O.

    2017-06-01

    The L'Espinau site is a dinosaur bonebed from the Upper Cretaceous of the South-Central Pyrenees (north-eastern Spain) that have provided hundreds of bone remains attributed to hadrosauroids, together with a rich assemblage of herpetofauna, fish and microflora. Magnetostratigraphy calibrated the site with the early late Maastrichtian, and the combined sedimentology, stable isotope geochemistry and palaeoecology revealed that this fossil site formed in a lagoon, in which a mixed freshwater-brackish palaeoenvironment was developed. This setting displays a south-north charophyte zonation from freshwater (Clavator brachycerus-dominated assemblage) to brackish or eurihaline conditions (Feistiella malladae-dominated assemblage), revealing a palaeoenvironment change towards the coast. Sedimentology and taphonomy (bidirectional arrangement of long bones, abrasion and disarticulation) indicate that the L'Espinau site is the result of a cohesive mass flow event originated very close to the sea. This process entrained and mixed fauna from both the terrestrial and the brackish/marine environment of a lagoon. An increasing of the water runoff (e.g. by intense rainfall) reworking poorly consolidated sediments is considered here as the most probable triggering mechanism. Mass flow-hosted bonebeds are commonly linked to fluvial palaeoenvironments, so our study case is a rare example of bones accumulating near the sea. This study adds evidence that hadrosauroids inhabited littoral environments during the Maastrichtian in the southern Pyrenean area.

  7. Allodaposuchus palustris sp. nov. from the upper cretaceous of Fumanya (South-Eastern Pyrenees, Iberian Peninsula: systematics, palaeoecology and palaeobiogeography of the enigmatic allodaposuchian crocodylians.

    Directory of Open Access Journals (Sweden)

    Alejandro Blanco

    Full Text Available The controversial European genus Allodaposuchus is currently composed of two species (A. precedens, A. subjuniperus and it has been traditionally considered a basal eusuchian clade of crocodylomorphs. In the present work, the new species A. palustris is erected on the base of cranial and postcranial remains from the lower Maastrichtian of the southern Pyrenees. Phylogenetic analyses here including both cranial and postcranial data support the hypothesis that Allodaposuchus is included within Crocodylia. The studied specimen suggests little change in postcranial skeleton along the evolutionary history of crocodylians, except for some bone elements such as the axis, the first caudal vertebra and the ilium. The specimen was found in an organic mudstone corresponding to a coastal wetland environment. Thus, A. palustris from Fumanya is the first Allodaposuchus reported in lacustrine-palustrine settings that expand the ecological range for this genus. The S-DIVA palaeobiogeographic reconstruction of ancestral area suggests that early members of Crocodylia rapidly widespread for the Northern Hemisphere landmasses no later than the Campanian, leading the apparition of endemic groups. In that way "Allodaposuchia" represents an endemic European clade probably originated in the Ibero-Armorican domain in the late Campanian and dispersed by the Southern European archipelago prior to the early Maastrichtian.

  8. Calculation of the rockwall recession rate of a limestone cliff, affected by rockfalls, using cosmogenic chlorine-36. Case study of the Montsec Range (Eastern Pyrenees, Spain)

    Science.gov (United States)

    Domènech, Guillem; Corominas, Jordi; Mavrouli, Olga; Merchel, Silke; Abellán, Antonio; Pavetich, Stefan; Rugel, Georg

    2018-04-01

    Cliff erosion may be a major problem in settled areas affecting populations and producing economic and ecological losses. In this paper we present a procedure to calculate the long-term retreat rate of a cliff affected by rockfalls in the Montsec Range, Eastern Pyrenees (Spain). It is composed of low, densely fractured limestones; and the rockwall is affected by rockfalls of different sizes. The rockfall scars are clearly distinguishable by their regular boundaries and by their orange colour, which contrast with the greyish old reference surface (S0) of the cliff face. We have dated different stepped surfaces of the rockwall, including S0, using cosmogenic 36Cl. The total amount of material released by rockfall activity was calculated using a high definition point cloud of the slope face obtained with a terrestrial laser scanner (TLS). The present rockwall surface has been subtracted from the reconstructed old cliff surface. This has allowed the calculation of the total volume released by rockfalls and of the retreat rate. The latter ranges from 0.31 to 0.37 mm·a- 1. This value is of the same order of magnitude as that obtained by other researchers in neighbouring regions in Spain, having similar geology and affected by rockfalls.

  9. Analysis and Predictability of the Hydrological Response of Mountain Catchments to Heavy Rain on Snow Events: A Case Study in the Spanish Pyrenees

    Directory of Open Access Journals (Sweden)

    Javier G. Corripio

    2017-03-01

    Full Text Available From 18 to 19 June 2013, the Ésera river in the Pyrenees, Northern Spain, caused widespread damage due to flooding as a result of torrential rains and sustained snowmelt. We estimate the contribution of snow melt to total discharge applying a snow energy balance to the catchment. Precipitation is derived from sparse local measurements and the WRF-ARW model over three nested domains, down to a grid cell size of 2 km. Temperature profiles, precipitation and precipitation gradient are well simulated, although with a possible displacement regarding the observations. Snowpack melting was correctly reproduced and verified in three instrumented sites, and according to satellite images. We found that the hydrological simulations agree well with measured discharge. Snowmelt represented 33% of total runoff during the main flood event and 23% at peak flow. The snow energy balance model indicates that most of the energy for snow melt during the day of maximum precipitation came from turbulent fluxes. This approach forecast correctly peak flow and discharge during normal conditions at least 24 h in advance and could give an early warning of the extreme event 2.5 days before.

  10. Synthesis report: program ecosystems, transport, pollutions, 1998 - 2001

    International Nuclear Information System (INIS)

    Etchelecou, A.; Deletraz, G.; Elichegaray, Ch.

    2001-04-01

    The ''Ecosystems, Transports, Pollution Program'' ETP Program, has been elaborated to evaluate the road pollution impacts on the mountain ecosystems. Four mountains valleys have been chosen: two in Alps (Chamonix and Maurienne) and two in Pyrenees (Biriatou and Vallee d'Aspe). This Program presents six objectives: the road traffic characterization, the pollutants emission estimation, the pollutants concentrations in the air, the pollutants dispersion according to relief, the relationships between pollutants emissions and bio-monitoring, the road pollution effects on nearby ecosystems. (A.L.B.)

  11. Synthesis report: program ecosystems, transport, pollutions, 1998 - 2001; Rapport de synthese: programme ecosystemes, transport, pollutions, 1998 - 2001

    Energy Technology Data Exchange (ETDEWEB)

    Etchelecou, A.; Deletraz, G.; Elichegaray, Ch

    2001-04-01

    The ''Ecosystems, Transports, Pollution Program'' ETP Program, has been elaborated to evaluate the road pollution impacts on the mountain ecosystems. Four mountains valleys have been chosen: two in Alps (Chamonix and Maurienne) and two in Pyrenees (Biriatou and Vallee d'Aspe). This Program presents six objectives: the road traffic characterization, the pollutants emission estimation, the pollutants concentrations in the air, the pollutants dispersion according to relief, the relationships between pollutants emissions and bio-monitoring, the road pollution effects on nearby ecosystems. (A.L.B.)

  12. Assessment of the performance of X-band satellite radar data for landslide mapping and monitoring: Upper Tena Valley case study

    Directory of Open Access Journals (Sweden)

    D. Notti

    2010-09-01

    Full Text Available The aim of this work is to analyse the advantages and disadvantages of using the new X-band SAR data acquired by TerraSAR-X sensors for landslides mapping. This dataset has been processed using a Persistent Scatterer Interferometry technique over the Upper Tena Valley (Central Pyrenees, Spain. In the first section, the geological and geomorphological setting of the study area is introduced, focusing on the description of the landslide inventory. Then the Stable Point Network technique is briefly described, followed by the assessment of the performance of the X-band SAR dataset. In this context, we present first a model to predict the distribution of Persistent Scatterers based on the slope geometry and the land use information, which has then been validated with X-band data results. On a second stage, we have assessed the performance of X-band dataset to detect and monitor mapped landslides. Finally some illustrative case studies are shown demonstrating the potential of using X-band SAR data not only for landslide mapping but also to detect and monitor deformations affecting human infrastructures.

  13. 76 FR 22746 - Conecuh Valley Railway, LLC-Acquisition and Operation Exemption-Conecuh Valley Railroad Co., Inc.

    Science.gov (United States)

    2011-04-22

    ... Surface Transportation Board Conecuh Valley Railway, LLC--Acquisition and Operation Exemption--Conecuh Valley Railroad Co., Inc. Conecuh Valley Railway, LLC (CVR), a noncarrier, has filed a verified notice of exemption under 49 CFR 1150.31 to acquire from Conecuh Valley Railroad Co., Inc. (COEH), and to operate...

  14. Valley-filtered edge states and quantum valley Hall effect in gated bilayer graphene.

    Science.gov (United States)

    Zhang, Xu-Long; Xu, Lei; Zhang, Jun

    2017-05-10

    Electron edge states in gated bilayer graphene in the quantum valley Hall (QVH) effect regime can carry both charge and valley currents. We show that an interlayer potential splits the zero-energy level and opens a bulk gap, yielding counter-propagating edge modes with different valleys. A rich variety of valley current states can be obtained by tuning the applied boundary potential and lead to the QVH effect, as well as to the unbalanced QVH effect. A method to individually manipulate the edge states by the boundary potentials is proposed.

  15. Minimal geometry for valley filtering in graphene

    Science.gov (United States)

    Asmar, Mahmoud M.; Ulloa, Sergio E.

    2017-11-01

    The possibility to effect valley splitting of an electronic current in graphene represents the essential component in the new field of valleytronics in such two-dimensional materials. Based on a symmetry analysis of the scattering matrix, we show that if the spatial distribution of multiple potential scatterers breaks mirror symmetry about the axis of incoming electrons, then a splitting of the current between two valleys is observed. This leads to the appearance of the valley Hall effect. We illustrate the effect of mirror-symmetry breaking in a minimal system of two symmetric impurities, demonstrating the splitting between valleys via the differential cross sections and nonvanishing skew parameter. We further discuss the role that these effects may play in transport experiments.

  16. VALMET-A valley air pollution model

    Energy Technology Data Exchange (ETDEWEB)

    Whiteman, C.D.; Allwine, K.J.

    1983-09-01

    Following a thorough analysis of meteorological data obtained from deep valleys of western Colorado, a modular air-pollution model has been developed to simulate the transport and diffusion of pollutants released from an elevated point source in a well-defined mountain valley during the nighttime and morning transition periods. This initial version of the model, named VALMET, operates on a valley cross section at an arbitrary distance down-valley from a continuous point source. The model has been constructed to include parameterizations of the major physical processes that act to disperse pollution during these time periods. The model has not been fully evaluated. Further testing, evaluations, and development of the model are needed. Priorities for further development and testing are provided.

  17. Meie ingel Silicon Valleys / Raigo Neudorf

    Index Scriptorium Estoniae

    Neudorf, Raigo

    2008-01-01

    Ettevõtluse Arendamise Sihtasutuse esinduse töölepanekust USAs Silicon Valleys räägib esinduse juht Andrus Viirg. Vt. ka: Eestlasi leidub San Franciscos omajagu; Muljetavaldav karjäär; USAga ammune tuttav

  18. Meie mees Silicon Valleys / Kertu Ruus

    Index Scriptorium Estoniae

    Ruus, Kertu, 1977-

    2007-01-01

    Ilmunud ka: Delovõje Vedomosti 5. dets. lk. 4. Peaminister Andrus Ansip avas Eesti Ettevõtluse Sihtasutuse esinduse Silicon Valley pealinnas San Joses. Vt. samas: Ränioru kliima on tehnoloogiasõbralik; Andrus Viirg

  19. Silicon Valley Smart Corridor : draft evaluation strategy

    Science.gov (United States)

    2000-06-05

    This document outlines the strategy for evaluating the integrated freeway, arterial, and incident management system known as the Silicon Valley Smart Corridor (SVSC). Centered in San Jose, California, the SVSC is one of approximately 65 deployments o...

  20. Burrowing Owl - Palo Verde Valley [ds197

    Data.gov (United States)

    California Department of Resources — These burrowing owl observations were collected during the spring and early summer of 1976 in the Palo Verde Valley, eastern Riverside County, California. This is an...

  1. Hydrothermal system of Long Valley caldera, California

    Energy Technology Data Exchange (ETDEWEB)

    Sorey, M.L.; Lewis, R.E.; Olmsted, F.H.

    1978-01-01

    The geologic and hydrologic setting of the hydrothermal system are described. The geochemical and thermal characteristics of the system are presented. A mathematical model of the Long Valley caldera is analyzed. (MHR)

  2. Statistical analysis of Amenamevir (ASP2151) between pharmacokinetics and clinical efficacies with non-linear effect model for the treatment of genital herpes.

    Science.gov (United States)

    Takada, Akitsugu; Katashima, Masataka; Kaibara, Atsunori; Sawamoto, Taiji; Zhang, Wenhui; Keirns, James

    2014-09-01

    Amenamevir is the international non-proprietary name for ASP2151 synthesized by Astellas Pharma, Inc. It is a structurally novel class of helicase-primase inhibitor and demonstrated more potency in vitro anti-viral activity with low cytotoxicity against varicella-zoster virus (VZV), herpes simplex virus type 1 (HSV-1), and herpes simplex virus type 2 (HSV-2) than acyclovir (ACV). Phase II randomized trial assessed the safety and efficacy of ASP2151 for episodic therapy of recurrent genital herpes was conducted. Participants self-initiated with ASP2151 (100, 200, or 400 mg daily for 3 days), ASP2151 (1,200 mg as a single dose), placebo for 3 days, or Valacyclovir (500 mg twice daily for 3 days). We present a first population pharmacokinetic (PPK) modeling analysis of Amenamevir for genital herpes patients. The final model retained the effect of Weight and Albumin on CL. Statistical analysis between pharmacokinetics and clinical efficacies was done by using the time above 200 ng/mL (T200 ). T200 derived from the final PPK model to consider the correlation with Time to lesion healing and viral shedding. This finding suggested that it could be necessary to maintain the Amenamevir concentration above the threshold level to prevent the virus replication. © 2014, The American College of Clinical Pharmacology.

  3. eNOS Glu298Asp polymorphism is associated with development of complicated plaques in patients from Serbia with advanced carotid atherosclerosis

    Directory of Open Access Journals (Sweden)

    Đurić Tamara

    2013-01-01

    Full Text Available Nitric oxide inhibits adhesion of thrombocytes, proliferation and migration of smooth muscle cells and restricts oxidation of atherogenic low-density lipoproteins. Therefore, decreased production or activity of NO may play a role in the initiation, progression or complications of atherosclerosis. The aim of this study was to estimate the effect of Glu298Asp eNOS gene polymorphism on the individual risk for development of complicated carotid atherosclerotic plaque in patients from Serbia with advanced carotid atherosclerosis (CA who had undergone endarterectomy. The study population included 233 patients. eNOS G894T gene polymorphism was identified by PCR and RFLP methods. Multivariate logistic regression analysis showed that Asp298Asp is an independent risk factor for the presence of complicated plaques in CA patients. Patients who were homozygous for the Asp298 allele had an adjusted OR of 4.36 for the development of complicated plaques compared to those that carry the Glu298 allele. Further validation and replication studies are needed. [Projekat Ministarstva nauke Republike Srbije, br. OI175085

  4. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination...

  5. Jutustuse ja romaani vahel: Elisabeth Aspe linn. Between a story and a novel : Elisabeth Aspe’ s town

    Directory of Open Access Journals (Sweden)

    Elle-Mari Talivee

    2012-06-01

    Full Text Available The following article analyses the description of towns in the short novels of Elisabeth Aspe: Kasuõde (Stepsister, 1887, Ennosaare Ain (Ain From Ennosaare Farm, 1888, Anna Dorothea (1891 and Aastate pärast (After Many Years, 1910. Aspe (1860–1927, one of the first Estonian female writers and an early realist, lived on the town border of Pärnu in south-west Estonia. During this historically interesting period, towns grew rapidly, but the ideology initiated by the national awakening movement concentrated on the idea of a nation being tightly bound to the countryside. Aspe’s works are like a litmus test of her time, and were influenced by German authors (Ottilie Wildermuth, W. Heimburg and Eugenie Marlitt, as well as by the Estonian national awakening period. Her first (epistolary novel is based on a pure opposition between the city and the country; in her later novels, the emphasis has changed: the contrasts are suddenly more ambivalent. One possible explanation is the impelling emergence of city thematics, and the early city novel replacing the village story – a process that began in Estonian literature at that time – resulting in a more complicated picture than a simple black-and-white opposition. This was a period of mapping, describing and exploring the townscape with curiosity and eagerness; also, it was a period of warning social criticism. For example, Eduard Vilde wrote a novel about factories consuming their workers, and men from country villages coming to a town. It was also a time of a fight for Estonia’s own town space, persuading the Estonian reader that he/she had the same right to live in towns, and even to govern towns, as the existing upper classes from other nationalities. In Ain From Ennosaare Farm, the protagonist has a double Estonian- German identity, belonging simultaneously to the town and to the countryside; finally, he acknowledges his ethnic origin. Throughout Aspe’s novels, the word “city” has a

  6. Strawberry Growing in the Pajaro Valley

    OpenAIRE

    Regional History Project, UCSC Library; Shikuma, Hiroshi; Jarrell, Randall

    1986-01-01

    Mr. Shikuma is a prominent Nisei strawberry grower in the Pajaro Valley. In this volume he describes family life in the Japanese-American community in the Pajaro Valley during the first decades of the twentieth century. He conveys the texture of everyday family life, recalling details of housing, food preparation, education, religion, and his childhood responsibilities in a farming family. The second part of the volume describes the growth and development of strawberries as an import...

  7. Intensification of IDegAsp Twice Daily (Adding Insulin Aspart vs. Switching To Basal-Bolus): Exploratory Randomized Trial in Type 2 Diabetes.

    Science.gov (United States)

    Bebakar, Wan Mohamaed Wan; Chaykin, Louis; Hersløv, Malene Lundgren; Rasmussen, Søren

    2017-02-01

    In a preceding trial comparing two different titration schemes, insulin degludec/insulin aspart (IDegAsp) showed good efficacy for achieving HbA 1c IAsp) or by switching to a basal-bolus regimen of insulin degludec (IDeg) plus IAsp taken three times a day (TID). A 26-week, randomized, open-label, phase 3b, treat-to-target trial in which 40 patients with T2D who had not reached target HbA 1c ≤7.0% following previous 26-week treatment intensification with IDegAsp BID ±3 oral antidiabetic agents (OADs) were randomized (1:1) to receive IDegAsp BID + IAsp OD (n = 20) or IDeg OD + IAsp TID (n = 20). Mean baseline HbA 1c was 7.9% in the IDegAsp BID + IAsp OD group and 7.7% in the IDeg OD + IAsp TID group. After 26 weeks, the estimated mean change in HbA 1c from baseline was 0.05% points in the IDegAsp BID + IAsp OD group and -0.49% points for IDeg OD + IAsp TID: estimated treatment difference (ETD) [95% confidence interval] 0.54% [0.09; 0.99], p = 0.021. Few achieved HbA 1c IAsp OD (four patients) and IDeg OD + IAsp TID groups (five patients). Fasting plasma glucose, hypoglycemia, and adverse events were similar between groups. When used as intensification regimens in patients who failed to achieve target HbA 1c during 26-week IDegAsp BID treatment, HbA 1c improvements were numerically greater with IDeg OD + IAsp TID compared with IDegAsp BID + IAsp OD. No new safety issues were identified. However, the small, selective sample means clinical generalizations should be made with caution. Novo Nordisk. CLINICALTRIALS. NCT01814137.

  8. Distinct Prandial and Basal Glucose-Lowering Effects of Insulin Degludec/Insulin Aspart (IDegAsp) at Steady State in Subjects with Type 1 Diabetes Mellitus.

    Science.gov (United States)

    Heise, Tim; Nosek, Leszek; Roepstorff, Carsten; Chenji, Suresh; Klein, Oliver; Haahr, Hanne

    2014-06-01

    Insulin degludec/insulin aspart (IDegAsp) is a soluble co-formulation of long-acting and short-acting insulin analogs. The primary objective of this study was to investigate the pharmacodynamic response of once-daily IDegAsp dosing in patients with type 1 diabetes. Pharmacokinetic response, as well as safety and tolerability, were assessed as secondary objectives. This was a single-center, open-label, single-arm study. Twenty-two subjects received once-daily insulin degludec (IDeg) (0.42 U/kg) for five consecutive days [with separate bolus insulin aspart (IAsp) as needed for safety and glycemic control], to achieve clinical steady state of the basal component. On Day 6, they received a single injection of IDegAsp (0.6 U/kg, comprising 0.42 U/kg IDeg and 0.18 U/kg IAsp). Pharmacodynamic response was assessed using a 30-h euglycemic glucose clamp, with blood glucose stabilized at a target of 5.5 mmol/L. The glucose infusion rate profile showed a rapid onset of action and a distinct peak due to IAsp, followed by a separate, flat and stable basal glucose-lowering effect due to the IDeg component. Modeling data suggested that the pharmacodynamic profile of IDegAsp was retained with twice-daily dosing (allowing for coverage of two main meals daily). IDegAsp was well tolerated and no safety issues were identified in this trial. In conclusion, the IAsp component of IDegAsp has a fast onset of appearance and a peak covering the prandial phase, while the IDeg component has a flat and an evenly distributed pharmacokinetic profile over 24 h. IDegAsp is the first co-formulation of a basal insulin analog with an ultra-long duration of action and a mealtime insulin analog in a single soluble injection. These properties translate into clinically relevant benefits, including improved glycemic control and reduction in hypoglycemia.

  9. ASPsiRNA: A Resource of ASP-siRNAs Having Therapeutic Potential for Human Genetic Disorders and Algorithm for Prediction of Their Inhibitory Efficacy.

    Science.gov (United States)

    Monga, Isha; Qureshi, Abid; Thakur, Nishant; Gupta, Amit Kumar; Kumar, Manoj

    2017-09-07

    Allele-specific siRNAs (ASP-siRNAs) have emerged as promising therapeutic molecules owing to their selectivity to inhibit the mutant allele or associated single-nucleotide polymorphisms (SNPs) sparing the expression of the wild-type counterpart. Thus, a dedicated bioinformatics platform encompassing updated ASP-siRNAs and an algorithm for the prediction of their inhibitory efficacy will be helpful in tackling currently intractable genetic disorders. In the present study, we have developed the ASPsiRNA resource (http://crdd.osdd.net/servers/aspsirna/) covering three components viz (i) ASPsiDb , (ii) ASPsiPred , and (iii) analysis tools like ASP-siOffTar ASPsiDb is a manually curated database harboring 4543 (including 422 chemically modified) ASP-siRNAs targeting 78 unique genes involved in 51 different diseases. It furnishes comprehensive information from experimental studies on ASP-siRNAs along with multidimensional genetic and clinical information for numerous mutations. ASPsiPred is a two-layered algorithm to predict efficacy of ASP-siRNAs for fully complementary mutant (Eff mut ) and wild-type allele (Eff wild ) with one mismatch by ASPsiPred SVM and ASPsiPred matrix , respectively. In ASPsiPred SVM , 922 unique ASP-siRNAs with experimentally validated quantitative Eff mut were used. During 10-fold cross-validation (10nCV) employing various sequence features on the training/testing dataset (T737), the best predictive model achieved a maximum Pearson's correlation coefficient (PCC) of 0.71. Further, the accuracy of the classifier to predict Eff mut against novel genes was assessed by leave one target out cross-validation approach (LOTOCV). ASPsiPred matrix was constructed from rule-based studies describing the effect of single siRNA:mRNA mismatches on the efficacy at 19 different locations of siRNA. Thus, ASPsiRNA encompasses the first database, prediction algorithm, and off-target analysis tool that is expected to accelerate research in the field of RNAi

  10. Role of enzymatic activity in muscle damage and cytotoxicity induced by Bothrops asper Asp49 phospholipase A2 myotoxins: are there additional effector mechanisms involved?

    Science.gov (United States)

    Mora-Obando, Diana; Díaz, Cecilia; Angulo, Yamileth; Gutiérrez, José María; Lomonte, Bruno

    2014-01-01

    Viperid venoms often contain mixtures of Asp49 and Lys49 PLA2 myotoxin isoforms, relevant to development of myonecrosis. Given their difference in catalytic activity, mechanistic studies on each type require highly purified samples. Studies on Asp49 PLA2s have shown that enzyme inactivation using p-bromophenacyl bromide (p-BPB) drastically affects toxicity. However, based on the variable levels of residual toxicity observed in some studies, it has been suggested that effector mechanisms independent of catalysis may additionally be involved in the toxicity of these enzymes, possibly resembling those of the enzymatically inactive Lys49 myotoxins. A possibility that Lys49 isoforms could be present in Asp49 PLA2 preparations exists and, if undetected in previous studies, could explain the variable residual toxicity. This question is here addressed by using an enzyme preparation ascertained to be free of Lys49 myotoxins. In agreement with previous reports, inactivation of the catalytic activity of an Asp49 myotoxin preparation led to major inhibition of toxic effects in vitro and in vivo. The very low residual levels of myotoxicity (7%) and cytotoxicity (4%) observed can be attributed to the low, although detectable, enzyme remaining active after p-BPB treatment (2.7%), and would be difficult to reconcile with the proposed existence of additional catalytic-independent toxic mechanisms. These findings favor the concept that the effector mechanism of toxicity of Asp49 PLA2 myotoxins from viperids fundamentally relies on their ability to hydrolyze phospholipids, arguing against the proposal that membrane disruption may also be caused by additional mechanisms that are independent of catalysis.

  11. Role of enzymatic activity in muscle damage and cytotoxicity induced by Bothrops asper Asp49 phospholipase A2 myotoxins: are there additional effector mechanisms involved?

    Directory of Open Access Journals (Sweden)

    Diana Mora-Obando

    2014-09-01

    Full Text Available Viperid venoms often contain mixtures of Asp49 and Lys49 PLA2 myotoxin isoforms, relevant to development of myonecrosis. Given their difference in catalytic activity, mechanistic studies on each type require highly purified samples. Studies on Asp49 PLA2s have shown that enzyme inactivation using p-bromophenacyl bromide (p-BPB drastically affects toxicity. However, based on the variable levels of residual toxicity observed in some studies, it has been suggested that effector mechanisms independent of catalysis may additionally be involved in the toxicity of these enzymes, possibly resembling those of the enzymatically inactive Lys49 myotoxins. A possibility that Lys49 isoforms could be present in Asp49 PLA2 preparations exists and, if undetected in previous studies, could explain the variable residual toxicity. This question is here addressed by using an enzyme preparation ascertained to be free of Lys49 myotoxins. In agreement with previous reports, inactivation of the catalytic activity of an Asp49 myotoxin preparation led to major inhibition of toxic effects in vitro and in vivo. The very low residual levels of myotoxicity (7% and cytotoxicity (4% observed can be attributed to the low, although detectable, enzyme remaining active after p-BPB treatment (2.7%, and would be difficult to reconcile with the proposed existence of additional catalytic-independent toxic mechanisms. These findings favor the concept that the effector mechanism of toxicity of Asp49 PLA2 myotoxins from viperids fundamentally relies on their ability to hydrolyze phospholipids, arguing against the proposal that membrane disruption may also be caused by additional mechanisms that are independent of catalysis.

  12. Challenges in pKa Predictions for Proteins: The case of Asp213 in Human Proteinase 3

    Science.gov (United States)

    Hajjar, Eric; Dejaegere, Annick; Reuter, Nathalie

    2009-09-01

    Knowledge of the protonation states of the ionizable residues in an enzyme is a prerequisite to an accurate description of its structure and mechanism. In practice, the use of the inappropriate protonation state for an amino acid in a molecular modeling computation (e.g., molecular dynamics simulation) is likely to lead to unrealistic results. Although methods using solvers of the linearized Poisson-Boltzmann equation have proven to yield accurate pKa predictions, they bear a number of limitations. They are quite demanding in terms of computational power and are sensitive to representation of the charges and their position (force field and protein conformation). Moreover they depend on the choice of a dielectric constant for the protein interior. In this manuscript, we describe the difficulties met when trying to predict the protonation state of a buried amino acid, located in a protein for which very little biochemical data is available. Such a case is highly representative of the challenges faced in theoretical biology studies. Proteinase 3 (PR3) is an enzyme involved in proteolytic events associated with inflammation. It is a potential target in the development of new anti-inflammatory therapeutic strategies. We report the results of pKa predictions of the aspartic acid 213 of PR3 with a FDPB solver. We probed the influence of the choice of the dielectric constant for the protein interior ɛp and the benefits of conformational sampling by molecular dynamics (MD) on the pKa prediction of this carboxylate group. Using only the FDPB calculations, we could not conclude on the protonation state of Asp213. MD simulations confronted to knowledge of the ligand-binding and reaction mechanism led us to decide on a protonated form of this aspartic acid. We also demonstrate that the use of the wrong protonation state leads to an unreliable structural model for PR3. pKa prediction with a fast empirical method yielded a pKa of 8.4 for Asp213, which is in agreement with our

  13. 77 FR 33237 - Saline Valley Warm Springs Management Plan/Environmental Impact Statement, Death Valley National...

    Science.gov (United States)

    2012-06-05

    ... visitor access, asset management, water resources, biological and cultural resources, human health and... desert valley located in the northwest portion of Death Valley National Park. Despite the variety of... passage of the California Desert Protection Act in 1994. Under NPS management, efforts have been made to...

  14. Topological Valley Transport in Two-dimensional Honeycomb Photonic Crystals.

    Science.gov (United States)

    Yang, Yuting; Jiang, Hua; Hang, Zhi Hong

    2018-01-25

    Two-dimensional photonic crystals, in analogy to AB/BA stacking bilayer graphene in electronic system, are studied. Inequivalent valleys in the momentum space for photons can be manipulated by simply engineering diameters of cylinders in a honeycomb lattice. The inequivalent valleys in photonic crystal are selectively excited by a designed optical chiral source and bulk valley polarizations are visualized. Unidirectional valley interface states are proved to exist on a domain wall connecting two photonic crystals with different valley Chern numbers. With the similar optical vortex index, interface states can couple with bulk valley polarizations and thus valley filter and valley coupler can be designed. Our simple dielectric PC scheme can help to exploit the valley degree of freedom for future optical devices.

  15. Low-frequency noise in δ13C and δ18O tree ring data: A case study of Pinus uncinata in the Spanish Pyrenees

    Science.gov (United States)

    Esper, Jan; Frank, David C.; Battipaglia, Giovanna; Büntgen, Ulf; Holert, Christopher; Treydte, Kerstin; Siegwolf, Rolf; Saurer, Matthias

    2010-12-01

    Isotopic discrimination measurements in tree rings are becoming increasingly important estimators of past environmental change. Potential biases inherent to these parameters, including age trend and level offset are, however, not well understood. We here perform measurements on a new millennium-long data set of decadally resolved δ18O and δ13C discrimination from 25 high-elevation pine trees in the Spanish Pyrenees to investigate whether such low-frequency biases exist and how they alter the long-term behavior of derived time series. Alignment of the tree ring data by biological age reveals age trends over the first one to four centuries after germination. On average, isotope values change by -0.089‰ δ18O and +0.064‰ δ13C per decade over the first 100 years of tree growth. This trend persists into the forth century after germination for δ18O but diminishes to ˜0‰ over the 100-390 year period for δ13C. We also find level offsets up to 7‰ δ18O and 3‰ δ13C between single trees. Analysis of the shape of age trends reveals negative exponential functions as reasonable choices for detrending of δ18O and (inverted) δ13C time series. The detrended isotope chronologies share low-frequency variance with traditional ring width and density measurements beyond statistical chance, suggesting that the various parameters reflect some of the same environmental forcing. Our results show that δ18O and δ13C from these Spanish pines need to be detrended to assess long-term environmental changes. To evaluate the general applicability of this conclusion, production of (1) well-replicated, (2) nonpooled, and (3) composite chronologies from other species and regions will be required. Increases in measurement speed and technology will make these tests feasible in the coming years.

  16. Epidemiological study of border disease virus infection in Southern chamois (Rupicapra pyrenaica) after an outbreak of disease in the Pyrenees (NE Spain).

    Science.gov (United States)

    Marco, Ignasi; Rosell, Rosa; Cabezón, Oscar; Mentaberre, Gregorio; Casas, Encarna; Velarde, Roser; López-Olvera, Jorge Ramón; Hurtado, Ana; Lavín, Santiago

    2008-02-05

    In 2001 and 2002, an outbreak of a previously unreported disease, associated with a border disease virus (BDV), caused high mortality in the Southern chamois (Rupicapra pyrenaica) population in the Alt Pallars-Aran National Hunting Reserve in the Catalan Pyrenees (NE Spain). Between 2002 and 2006, sera and/or tissue samples taken from 116 healthy chamois shot during the hunting season, plus 42 from chamois affected by different diseases, were studied. A blocking enzyme-immunosorbent assay (ELISA) was used to study pestivirus seroprevalence in 114 healthy hunted and 31 diseased chamois, yielding positive results in 73.7 and 22.6% of the chamois, respectively. Comparative virus neutralization tests (VNT) performed on 42 seropositive samples with 6 pestivirus strains yielded statistically higher titres to BDV Spain 97, followed by BDV chamois, BDV 137/4, BDV Moredun, Bovine Diarrhoea virus-1 (BVDV-1) NADL and BVDV-2 atypical. Virological investigations for pestivirus detection were performed using an antigen ELISA test in 82 healthy and 18 diseased chamois, RT-PCR in 16 healthy and in all diseased chamois, and virus isolation in 14 diseased chamois. No viral antigen was detected in any of the healthy animals. A pestivirus, characterized as BDV by monoclonal antibodies, was detected in the 10 chamois showing clinical signs consistent with BDV infection. Sequence analysis in the 5' untranslated region (5'-UTR) revealed that they were grouped into the BDV-4 genotype. In the remaining chamois, infectious keratoconjunctivitis, pneumonia, trauma and contagious ecthyma were diagnosed. The cause of death was unknown in five chamois. The results suggest that the infection has become endemic in the population and that it could have a significant impact on chamois population dynamics.

  17. Microbial plankton assemblages, composition and biomass, during two ice-free periods in a deep high mountain lake (Estany Redó, Pyrenees

    Directory of Open Access Journals (Sweden)

    Silvana HALAC

    1999-08-01

    Full Text Available Microbial plankton composition and biomass were monitored for two ice-free periods in a deep oligotrophic high-mountain lake (Redó, Pyrenees. Phytoplankton dominated microbial biomass, while the relationship between total water-column-integrated autotrophic and heterotrophic biomass ranged from 1.5 to 6.5 (an average of 4.4. Heterotrophic biomass was dominated by bacteria (an average of 47 %, but heterotrophic nanoflagellates and, to a lesser degree, ciliates occasionally constituted a sizeable proportion. In general, the microbial biomass ratios were 10:2:2:1 for PHY:BAC:HNF:CIL. About one hundred eukaryotic species were found, although most of them in low abundance and frequency. Phytoplankton biomass was dominated by flagellated chrysophytes and dinoflagellates (an average of 40 and 32% respectively; occasionally cryptophytes (in deep layers and chlorococcal chlorophytes (during the autumn mixing period were also significant. In the two years sampled, the maximum phytoplankton diversity was observed during the autumn mixing period. Heterotrophic flagellate biomass was dominated by chrysophytes (78% on average, but sporadically a non-identified species reached high abundances. Oligotrichs, (an average of 43% of total ciliate biomass dominated the ciliate community, still other groups (gymnostomatida and prostomatida were also significant. Bacteria biomass was largely homogeneous throughout the two periods, but size segregation was observed especially when the lake was stratified, with larger bacteria appearing in the upper layers. The highest planktonic microbial biomass occurred during the mixing periods, mainly during spring. But no clear relationships were found between the temporal distribution of bacteria, phytoplankton, heterotrophic flagellate and ciliate biomass.

  18. Case Study: Effect of Climatic Characterization on River Discharge in an Alpine-Prealpine Catchment of the Spanish Pyrenees Using the SWAT Model

    Directory of Open Access Journals (Sweden)

    Leticia Palazón

    2016-10-01

    Full Text Available The new challenges in assessment of water resources demand new approaches and tools, such as the use of hydrologic models, which could serve to assist managers in the prediction, planning and management of catchment water supplies in view of increased demand of water for irrigation and climatic change. Good characterization of the spatial patterns of climate variables is of paramount importance in hydrological modelling. This is especially so when modelling mountain environments which are characterized by strong altitudinal climate gradients. However, very often there is a poor distribution of climatic stations in these areas, which in many cases, results in under representation of high altitude areas with respect to climatic data. This results in the poor performance of the models. In the present study, the Soil and Water Assessment Tool (SWAT model was applied to the Barasona reservoir catchment in the Central Spanish Pyrenees in order to assess the influence of different climatic characterizations in the monthly river discharges. Four simulations with different input data were assessed, using only the available climate data (A1; the former plus one synthetic dataset at a higher altitude (B1; and both plus the altitudinal climate gradient (A2 and B2. The model’s performance was evaluated against the river discharges for the representative periods of 2003–2005 and 1994–1996 by means of commonly used statistical measures. The best results were obtained using the altitudinal climate gradient alone (scenario A2. This study provided insight into the importance of taking into account the sources and the spatial distribution of weather data in modelling water resources in mountainous catchments.

  19. 238U, 226Ra, 210Pb, 232Th and 40K activities in soil profiles of the Flysch sector (Central Spanish Pyrenees)

    International Nuclear Information System (INIS)

    Navas, A.; Soto, J.; Machin, J.

    2002-01-01

    Distributions of natural gamma-emitting radionuclides were determined in three soil profiles developed on Tertiary sedimentary materials in mountain landscapes of the Central Spanish Pyrenees. Radioisotope activities (Bq kg -1 ) lie in the range of 0-53 for 238 U; 19-33 for 226 Ra; 7-75 for 210 Pb; 24-48 for 232 Th and 335-562 for 40 K. 238 U and 210 Pb activities show an important variability down the soil profiles. 238 U was markedly depleted in all upper soil layers and highly enriched in lower layers of two soil profiles. 210 Pb exhibits very dissimilar distribution patterns in all three soils. 226 Ra and 232 Th had quite uniform depth distributions. 40 K showed depletion in both upper and lower layers in one soil profile but remained fairly constant in the other two profiles. 238 U/ 226 Ra activity ratios (ARs) have been used to assess equilibrium in the 238 U decay chain and as indicators of edaphogenesis in the studied soil profiles. Maintenance of initial proportionality in the ratio of 232 Th/ 238 U activities has been assessed through ARs of their progenies. Additionally, a variety of soil properties were measured down the soil profiles. Among soils, variation in radionuclide activities may be due to differences in carbonate content, organic matter and/or grain size. In this environment, soil properties differently affect mobilization of natural radionuclides. The association of some radiologic properties with soil layers suggest a relationship between soil processes and radionuclide distribution

  20. Paleozoic-involving thrust array in the central Sierras Interiores (South Pyrenean Zone, Central Pyrenees): regional implications

    Science.gov (United States)

    Rodriguez, L.; Cuevas, J.; Tubía, J. M.

    2012-04-01

    This work deals with the structural evolution of the Sierras Interiores between the Tena and Aragon valleys. The Sierras Interiores is a WNW-trending mountain range that bounds the South Pyrenean Zone to the north and that is characterized by a thrust-fold system with a strong lithological control that places preferably decollements in Triassic evaporites. In the studied area of the Sierras Interiores Cenomanian limestones cover discordantly the Paleozoic rocks of the Axial Zone because there is a stratigraphic lacuna developed from Triassic to Late Cretaceous times. A simple lithostratigraphy of the study area is made up of Late Cenomanian to Early Campanian limestones with grey colour and massive aspect in landscape (170 m, Lower calcareous section), Campanian to Maastrichtian brown coloured sandstones (400-600 m, Marboré sandstones) and, finally, Paleocene light-coloured massive limestones (130-230 m), that often generate the higher topographic levels of the Sierras Interiores due to their greater resistance to erosion. Above the sedimentary sequence of the Sierras Interiores, the Jaca Basin flysch succession crops out discordantly. Based on a detailed mapping of the studied area of the Sierras Interiores, together with well and structural data of the Jaca Basin (Lanaja, 1987; Rodríguez and Cuevas, 2008) we have constructed a 12 km long NS cross section, approximately parallel to the movement direction deduced for this region (Rodríguez et al., 2011). The main structure is a thrust array made up of at least four Paleozoic-involving thrusts (the deeper thrust system) of similar thickness in a probably piggyback sequence, some of which are blind thrusts that generate fold-propagation-folds in upper levels. The higher thrust of the thrust array crops out duplicating the lower calcareous section all over the Sierras Interiores. The emplacement of the deeper thrust system generated the tightness of previous structures: south directed piggyback duplexes (the upper

  1. Christmas Valley Renewable Energy Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Del Mar, Robert [Oregon Department of Energy, Salem, OR (United States)

    2017-05-22

    In partnership with the Oregon Military Department, the Department of Energy used the award to assess and evaluate renewable resources in a 2,622-acre location in Lake County, central Oregon, leading to future development of up to 200 MW of solar electricity. In partnership with the Oregon Military Department, the Department of Energy used the award to assess and evaluate renewable resources in a 2,622-acre location in Lake County, central Oregon, leading to future development of up to 200 MW of solar electricity. The Oregon Military Department (Military) acquired a large parcel of land located in south central Oregon. The land was previously owned by the US Air Force and developed for an Over-the-Horizon Backscatter Radar Transmitter Facility, located about 10 miles east of the town of Christmas Valley. The Military is investigating a number of uses for the site, including Research and Development (R&D) laboratory, emergency response, military operations, developing renewable energy and related educational programs. One of the key potential uses would be for a large scale solar photovoltaic power plant. This is an attractive use because the site has excellent solar exposure; an existing strong electrical interconnection to the power grid; and a secure location at a moderate cost per acre. The project objectives include: 1. Site evaluation 2. Research and Development (R&D) facility analysis 3. Utility interconnection studies and agreements 4. Additional on-site renewable energy resources analysis 5. Community education, outreach and mitigation 6. Renewable energy and emergency readiness training program for veterans

  2. Effects of Asn318 and Asp87Asn318 mutations on signal transduction by the gonadotropin-releasing hormone receptor and receptor regulation.

    Science.gov (United States)

    Awara, W M; Guo, C H; Conn, P M

    1996-02-01

    GnRH receptor (GnRH-R) contains Asn87 and Asp318 instead of the more frequently observed Asp87 and Asn318 found in other G protein-coupled receptors. In the present study, site-directed mutagenesis was used to introduce Asn318 and Asp87Asn318 into GnRH-R. The effect on coupling and regulation of GnRH-R was studied by stable expression of wild and mutant mouse GnRH-R in the lactotropic GH3 cells; these normally release PRL in response to TRH stimulation. The responses to Buserelin (a metabolically stable GnRH analog) in three different cell lines, M1, N8, and ND1 (expressing wild-type, Asn318 mutant, and Asp87Asn318 mutant mouse GnRH-R, respectively) were compared with that observed in the previously characterized GGH3-1' cells, which stably express rat GnRH-R. The Asn318 and Asp87Asn318 mutations had no measurable effect on ligand binding, but abolished the initial down-regulation of receptor that was observed in M1 and GGH3-1' cells, suggesting that the normal location of Asn87 and Asp318 in GnRH-R is involved in the regulation of GnRH-R. In N8 and ND1 cells, Buserelin-stimulated inositol phosphate (IP) production was attenuated, but the release of both cAMP and PRL was stimulated in a dose- and time-dependent manner. These mutations apparently impaired the coupling between GnRH-R and G proteins involved in IP production, but not those involved in cAMP release. In M1 cells, Buserelin stimulation produced a significant increase in IP production, but neither cAMP nor PRL release was significantly stimulated. These findings are consistent with the previous suggestion that GnRH-stimulated PRL release is mediated by a cAMP second messenger system in transfected GGH3 cells.

  3. Graphene Nanobubbles as Valley Filters and Beam Splitters

    DEFF Research Database (Denmark)

    Settnes, Mikkel; Power, Stephen; Brandbyge, Mads

    2016-01-01

    The energy band structure of graphene has two inequivalent valleys at the K and K' points of the Brillouin zone. The possibility to manipulate this valley degree of freedom defines the field of valleytronics, the valley analogue of spintronics. A key requirement for valleytronic devices is the ab......The energy band structure of graphene has two inequivalent valleys at the K and K' points of the Brillouin zone. The possibility to manipulate this valley degree of freedom defines the field of valleytronics, the valley analogue of spintronics. A key requirement for valleytronic devices...... is the ability to break the valley degeneracy by filtering and spatially splitting valleys to generate valley polarized currents. Here, we suggest a way to obtain valley polarization using strain-induced inhomogeneous pseudomagnetic fields (PMFs) that act oppositely on the two valleys. Notably, the suggested...... to be addressed individually. In this way, graphene nanobubbles can be exploited in both valley filtering and valley splitting devices, and our simulations reveal that a number of different functionalities are possible depending on the deformation field....

  4. Subsurface Salts in Antarctic Dry Valley Soils

    Science.gov (United States)

    Englert, P.; Bishop, J. L.; Gibson, E. K.; Koeberl, C.

    2013-01-01

    The distribution of water-soluble ions, major and minor elements, and other parameters were examined to determine the extent and effects of chemical weathering on cold desert soils. Patterns at the study sites support theories of multiple salt forming processes, including marine aerosols and chemical weathering of mafic minerals. Periodic solar-mediated ionization of atmospheric nitrogen might also produce high nitrate concentrations found in older sediments. Chemical weathering, however, was the major contributor of salts in Antarctic Dry Valleys. The Antarctic Dry Valleys represent a unique analog for Mars, as they are extremely cold and dry desert environments. Similarities in the climate, surface geology, and chemical properties of the Dry Valleys to that of Mars imply the possible presence of these soil formation mechanisms on Mars, other planets and icy satellites.

  5. The lakes of the Jordan Rift Valley

    International Nuclear Information System (INIS)

    Gat, J.R.

    2001-01-01

    This paper presents a summary of the proceedings of a workshop on the Lakes of the Jordan Rift Valley that was held in conjunction with the CRP on The Use of Isotope Techniques in Lake Dynamics Investigations. The paper presents a review of the geological, hydrogeological and physical limnological setting of the lakes in the Jordan Rift Valley, Lake Hula, Lake Kinneret and the Dead Sea. This is complemented by a description of the isotope hydrology of the system that includes the use of a wide range of isotopes: oxygen-18, deuterium, tritium, carbon-14, carbon-13, chlorine isotopes, boron-11 and helium-3/4. Environmental isotope aspects of the salt balances of the lakes, their palaeolimnology and biogeochemical tracers are also presented. The scope of application of isotopic tracers is very broad and provides a clear insight into many aspects of the physical, chemical and biological limnology of the Rift Valley Lakes. (author)

  6. A new Proposal to Mexico Valley Zonification

    Science.gov (United States)

    Flores-Estrella, H. C.; Yussim, S.; Lomnitz, C.

    2004-12-01

    The effects of the Michoacan earthquake (19th September, 1985, Mw 8.1) in Mexico City caused a significant change in the political, social and scientific history, as it was considered the worst seismic disaster ever lived in Mexico. Since then, numerous efforts have been made to understand and determine the parameters that caused the special features registered. One of these efforts had began on 1960 with the work by Marsal and Masari, who published the Mexico Valley seismological and geotechnical zonification (1969), based on gravimetric and shallow borehole data. In this work, we present a revision of the studies that proposed the zonification, a description of the valley geology, and basing on it we propose a new zonification for Mexico Valley.

  7. Geologic summary of the Owens Valley drilling project, Owens and Rose Valleys, Inyo County, California

    International Nuclear Information System (INIS)

    Schaer, D.W.

    1981-07-01

    The Owens Valley Drilling Project consists of eight drill holes located in southwest Inyo County, California, having an aggregate depth of 19,205 feet (5853 m). Project holes penetrated the Coso Formation of upper Pliocene or early Pleistocene age and the Owens Lake sand and lakebed units of the same age. The project objective was to improve the reliability of uranium-potential-resource estimates assigned to the Coso Formation in the Owens Valley region. Uranium-potential-resource estimates for this area in $100 per pound U 3 O 8 forward-cost-category material have been estimatd to be 16,954 tons (15,384 metric tons). This estimate is based partly on project drilling results. Within the Owens Valley project area, the Coso Formation was encountered only in the Rose Valley region, and for this reason Rose Valley is considered to be the only portion of the project area favorable for economically sized uranium deposits. The sequence of sediments contained in the Owens Valley basin is considered to be largely equivalent but lithologically dissimilar to the Coso Formation of Haiwee Ridge and Rose Valley. The most important factor in the concentration of significant amounts of uranium in the rock units investigated appears to be the availability of reducing agents. Significant amounts of reductants (pyrite) were found in the Coso Formation. No organic debris was noted. Many small, disconnected uranium occurrences, 100 to 500 ppM U 3 O 8 , were encountered in several of the holes

  8. Groundwater quality in Coachella Valley, California

    Science.gov (United States)

    Dawson, Barbara J. Milby; Belitz, Kenneth

    2012-01-01

    Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Coachella Valley is one of the study areas being evaluated. The Coachella study area is approximately 820 square miles (2,124 square kilometers) and includes the Coachella Valley groundwater basin (California Department of Water Resources, 2003). Coachella Valley has an arid climate, with average annual rainfall of about 6 inches (15 centimeters). The runoff from the surrounding mountains drains to rivers that flow east and south out of the study area to the Salton Sea. Land use in the study area is approximately 67 percent (%) natural, 21% agricultural, and 12% urban. The primary natural land cover is shrubland. The largest urban areas are the cities of Indio and Palm Springs (2010 populations of 76,000 and 44,000, respectively). Groundwater in this basin is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay derived from surrounding mountains. The primary aquifers in Coachella Valley are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database. Public-supply wells in Coachella Valley are completed to depths between 490 and 900 feet (149 to 274 meters), consist of solid casing from the land surface to a depth of 260 to 510 feet (79 to 155 meters), and are screened or perforated below the solid casing. Recharge to the groundwater system is primarily runoff from the surrounding mountains, and by direct infiltration of irrigation. The primary sources of discharge are pumping wells, evapotranspiration, and underflow to

  9. Genome analysis of Rift Valley fever virus, Mayotte.

    Science.gov (United States)

    Cêtre-Sossah, Catherine; Zeller, Hervé; Grandadam, Marc; Caro, Valérie; Pettinelli, François; Bouloy, Michèle; Cardinale, Eric; Albina, Emmanuel

    2012-06-01

    As further confirmation of a first human case of Rift Valley fever in 2007 in Comoros, we isolated Rift Valley fever virus in suspected human cases. These viruses are genetically closely linked to the 2006-2007 isolates from Kenya.

  10. Solar energy innovation and Silicon Valley

    Science.gov (United States)

    Kammen, Daniel M.

    2015-03-01

    The growth of the U. S. and global solar energy industry depends on a strong relationship between science and engineering innovation, manufacturing, and cycles of policy design and advancement. The mixture of the academic and industrial engine of innovation that is Silicon Valley, and the strong suite of environmental policies for which California is a leader work together to both drive the solar energy industry, and keep Silicon Valley competitive as China, Europe and other area of solar energy strength continue to build their clean energy sectors.

  11. Clean Cities Award Winning Coalition: Coachella Valley

    Energy Technology Data Exchange (ETDEWEB)

    ICF Kaiser

    1999-05-20

    Southern California's Coachella Valley became a Clean Cities region in 1996. Since then, they've made great strides. SunLine Transit, the regional public transit provider, was the first transit provider to replace its entire fleet with compressed natural gas buses. They've also built the foundation for a nationally recognized model in the clean air movement, by partnering with Southern California Gas Company to install a refueling station and developing a curriculum for AFV maintenance with the College of the Desert. Today the valley is home to more than 275 AFVs and 15 refueling stations.

  12. The crystal structure, mutagenesis, and activity studies reveal that patatin is a lipid acyl hydrolase with a Ser-Asp catalytic dyad.

    Science.gov (United States)

    Rydel, Timothy J; Williams, Jennifer M; Krieger, Elysia; Moshiri, Farhad; Stallings, William C; Brown, Sherri M; Pershing, Jay C; Purcell, John P; Alibhai, Murtaza F

    2003-06-10

    Patatin is a nonspecific lipid acyl hydrolase that accounts for approximately 40% of the total soluble protein in mature potato tubers, and it has potent insecticidal activity against the corn rootworm. We determined the X-ray crystal structure of a His-tagged variant of an isozyme of patatin, Pat17, to 2.2 A resolution, employing SeMet multiwavelength anomalous dispersion (MAD) phasing methods. The patatin crystal structure has three molecules in the asymmetric unit, an R-factor of 22.0%, and an R(free) of 27.2% (for 10% of the data not included in the refinement) and includes 498 water molecules. The structure notably revealed that patatin has a Ser-Asp catalytic dyad and an active site like that of human cytosolic phospholipase A(2) (cPLA(2)) [Dessen, A., et al. (1999) Cell 97, 349-360]. In addition, patatin has a folding topology related to that of the catalytic domain of cPLA(2) and unlike the canonical alpha/beta-hydrolase fold. The structure confirms our site-directed mutagenesis and bioactivity data that initially suggested patatin possessed a Ser-Asp catalytic dyad. Alanine-scanning mutagenesis revealed that Ser77 and Asp215 were critical for both esterase and bioactivity, consistent with prior work implicating a Ser residue [Strickland, J. H., et al. (1995) Plant Physiol. 109, 667-674] and a Ser-Asp dyad [Hirschberg, H. J. H. B., et al. (2001) Eur. J. Biochem. 268, 5037-5044] in patatin's catalytic activity. The crystal structure aids the understanding of other structure-function relationships in patatin. Patatin does not display interfacial activation, a hallmark feature of lipases, and this is likely due to the fact that it lacks a flexible lid that can shield the active site.

  13. Preliminary results from ASP on tests of QED to order α4 in e+e- annihilation at √s = 29 GeV

    International Nuclear Information System (INIS)

    Hawkins, C.A.

    1988-11-01

    Tests of QED to order α 4 performed with the ASP detector at PEP are presented. Measurements have been made of exclusive e + e - e + e - , e + e - γγ and γγγγ final states with all particles above 50 milliradians with respect to the e + e - beam line. These measurements represent a significant increase in statistics over previous measurements. All measurements agree well with theoretical predictions. 5 refs., 1 tab

  14. Molecular gallium arsenide phosphide clusters prepared from AsP(3), P(4), and [{GaC(SiMe(3))(3)}(4)].

    Science.gov (United States)

    Cossairt, Brandi M; Cummins, Christopher C

    2010-11-08

    Treatment of AsP(3) with 0.75 equivalents of [{GaC(SiMe(3))(3)}(4)] resulted in selective insertion of three equivalents of {GaC(SiMe(3))(3)} into the three As--P bonds to give [As{GaC(SiMe(3))(3)}(3)P(3)] (1-As) with an intact cyclo-P(3) ring. This yellow compound has been characterized by NMR spectroscopy, combustion analysis, single-crystal X-ray diffraction, UV/Vis spectroscopy, Raman spectroscopy, and cyclic voltammetry (THF, 0.2 M [TBA][B(C(6)F(5))(4)]; TBA=tetrabutyl ammonium). Computational models of 1-As and the isomeric [P{GaC(SiMe(3))(3)}(3)AsP(2)] (1-P) have been investigated as well, revealing several interesting electronic features of these cage molecules. Following from the cyclic voltammetry studies of 1-As that highlight an irreversible two-electron reduction at -2.2 V versus Fc/Fc(+), treatment with one equivalent of [Mg(C(14)H(10))(thf)(3)] resulted in two-electron reduction to provide [As{GaC(SiMe(3))(3)}(3)P(3)Mg(thf)(3)] (2), in which the Mg(2+) ion has inserted into one of the P--P bonds of the cyclo-P(3) ring. It was also found that treatment of AsP(3) or P(4) with one equivalent of [{GaC(SiMe(3))(3)}(4)] resulted in formation of the quadruple insertion products [As{GaC(SiMe(3))(3)}(4)P(3)] (3) and [P{GaC(SiMe(3))(3)}(4)P(3)] (4), respectively.

  15. Muscle phospholipid hydrolysis by Bothrops asper Asp49 and Lys49 phospholipase A₂ myotoxins--distinct mechanisms of action.

    Science.gov (United States)

    Fernández, Julián; Caccin, Paola; Koster, Grielof; Lomonte, Bruno; Gutiérrez, José M; Montecucco, Cesare; Postle, Anthony D

    2013-08-01

    Bothrops snakes are the major cause of ophidian envenomings in Latin America. Their venom contains myotoxins that cause prominent muscle damage, which may lead to permanent disability. These toxins include myotoxins Mt-I and Mt-II, which share the phospholipase A₂ (PLA₂) fold, but Mt-II lacks enzymatic activity because the essential active site Asp49 is replaced by Lys. Both myotoxins cause sarcolemma alterations, with Ca²⁺ entry and loss of ATP and K⁺ from muscle cells, but the molecular lesions at the basis of their cellular action are not known, particularly the role of phospholipid hydrolysis. Here we tested their PLA₂ activity in vivo, and evaluated the hypothesis that Ca²⁺-activated endogenous PLA₂s may be involved in the action of Mt-II. The time course of phospholipid hydrolysis by Mt-I and Mt-II in myotubes in culture and in tibialis anterior mouse muscles was determined. Mt-I rapidly hydrolyzed phosphatidylcholine and phosphatidylethanolamine but not phosphatidylserine, but no phospho-lipids were hydrolyzed in the presence of Mt-II. Whole Bothrops asper venom induced a higher extent of phospholipid hydrolysis than Mt-I alone. These results demonstrate in vivo PLA₂ activity of Mt-I for the first time, and indicate that it acts only on the external monolayer of the sarcolemma. They also exclude activation of endogenous PLA₂s in the action of Mt-II, implying that plasma membrane disruption by this toxin does not depend on phospholipid hydrolysis. Therefore, both Bothrops myotoxins induce Ca²⁺ entry and release of ATP and cause myonecrosis, but through different biochemical mechanisms. © 2013 FEBS.

  16. 124I labeled Arg-Gly-Asp (RGD) peptide: PET/MR fusion imaging of tumor expressing integrin v3

    International Nuclear Information System (INIS)

    Yun, Seon Young; Park, Jeong Chan; Chen, Xiaoyuan

    2007-01-01

    Tumor-induced angiogenesis and metastasis express high level of integrin v3 which has become a promising diagnostic biomarker and therapeutic target for various tumors. Radiolabeled RGD (Arg-Gly-Asp) peptides are well known to specifically bind to integrin and can be used not only for noninvasive imaging of integrin over-expression but also for integrin-targeted radionuclide therapy. We used three RGDyK derivatives, monomeric-, dimeric- and tetrameric-RGD for radio labeling. They were labeled with [ 124 I]NaI using iodo-bead. Labeled RGD peptide was purified by FPLC. Xenografts were induced by subcutaneous injection of 1X10 7 U87MG cells into the right thigh of female Balb/c nude mice. After injection of 124 I-labeled monomeric-RGD via the tail vein, the microPET imaging was obtained during 2 hours. Right after microPET imaging, T 2 -weighted MR imaging was performed with fast gradient spin echo sequence in 0.8 mm of a slice thickness. PET/MR fusion images were constructed by AMIDE program. For immobilization and reproducible positioning of animal, animal-specific positioning molds were fabricated. Monomeric-RGD peptide was labeled with 124 I in labeling yield of 50%. After FPLC purification, the radiochemical purity was above 90%. U87MG tumor was clearly seen in both microPET and MR imaging of tumor-bearing nude mouse. The fusion imaging was well constructed by using AMIDE with little discrepancy thanks to animal-specific molds. Three RGD derivatives were also labeled with 131 I in labeling yield of 20-55%. All three RGD peptides were successfully radioiodinated with 124 I or 131 I. U87MG glioblastoma tumor was clearly visualized in PET/MR fusion imaging. MicroPET/MR fusion imaging of dimeric- and tetrameric-RGD peptides in tumor bearing mice will also be presented during conference

  17. Identification of hemoglobin Q India (alpha 1-64 Asp-His) through ARMS-PCR. First report from Pakistan.

    Science.gov (United States)

    Moiz, Bushra; Moatter, Tariq; Hashmi, Mashhooda Rasool; Hashmi, Nazish; Kauser, Toheed; Nasir, Amna; Khurshid, Mohammad

    2008-05-01

    Various hemoglobinopathies have been reported from Pakistan excepting the rare ones like hemoglobin Q India. Our purpose of study was to identify the mutation (alpha 1 64 aspartate to histidine) through amplification restriction mutation system-polymerase chain reaction (ARMS-PCR) in patients where hemoglobin Q has been detected via high performance liquid chromatography (HPLC) and also to evaluate the cost effectiveness of the two technologies. All patients irrespective of age and gender who underwent HPLC for identification of their hemoglobin variant during January 1, 2006 to January 30, 2007 were studied. The blood samples with unknown peak at a retention time of 4.7 min were evaluated at the molecular level. Analysis of HPLC tracings of 11,008 subjects over a thirteen-month period identified ten individuals with hemoglobin Q. Male to female ratio was 1:1.5 and their age was variable ranging from 1 to 49 (mean 22.8) years. The mean hemoglobin level was 11.3 g/dl while MCV (fl) and MCH (pg) were 73.0 and 20.8 respectively. HPLC showed an unknown peak of 17.7% which was detected as Hb Q. ARMS based PCR showed Hb Q specific product of 370 bp and also an amplified product of 766 bp as the control fragment in these samples. This is the first ever report that documents the presence of Hb Q India (alpha 64 Asp to His) in Pakistani population. We recommend that HPLC be used as a useful screening tool especially in developing countries where PCR facilities may not be accessible.

  18. Comparison of intracellular signalling by insulin and the hypermitogenic AspB10 analogue in MCF-7 breast adenocarcinoma cells.

    Science.gov (United States)

    Oleksiewicz, Martin B; Bonnesen, Christine; Hegelund, Anne Charlotte; Lundby, Anders; Holm, Gitte-Mai Nelander; Jensen, Marianne B; Krabbe, Jonas S

    2011-05-01

    We compared mitogenicity and intracellular signalling by human insulin and the AspB10 (X-10) human insulin analogue in MCF-7 human mammary adenocarcinoma cells. By flow analysis of phosphorylated histone H3 or cell cycle distributions, insulin and X-10 were mitogenic at physiologically relevant concentrations (2 nm to 74 pm range), with X-10 being approximately 3-fold more mitogenic than insulin. By western blotting with phospho-specific antibodies, insulin induced phosphorylation of IRS-1, Akt, p70S6K, S6 ribosomal protein, 4E-BP1, FoxO3a, FoxO1, p44/42 MAPK and the EGFR. Blocking with wortmannin, rapamycin and U0126 showed that these signalling events conformed to the canonical PI3K pathway. IRS-1 (Ser302) phosphorylation was abolished by wortmannin and rapamycin, suggesting a feedback from the PI3K pathway on insulin signalling. Compared with equimolar insulin, X-10 caused up to 2-fold higher phosphorylation of all proteins examined in this study. The phosphorylation sites that responded most strongly to insulin were not generally the same as those responding most strongly to X-10. In the PI3K pathway, the most X-10-sensitive protein localized to the translation-regulating arm (p70S6K), with FoxO3a and FoxO1 transcription factors showing a more comparable response to insulin and X-10. Using flow analysis, we confirmed the correlation between insulin-triggered translational activation in G0/G1 (S6 phosphorylation) and S-phase entry by MCF-7 cells. In summary, our findings implicate asymmetrical PI3K pathway activation and specifically stimulation of protein translation in the hypermitogenic effect of insulin analogues such as X-10. It remains to be shown whether these findings are relevant to other human mammary cancer cell types. Copyright © 2010 John Wiley & Sons, Ltd.

  19. The Rho ADP-ribosylating C3 exoenzyme binds cells via an Arg-Gly-Asp motif.

    Science.gov (United States)

    Rohrbeck, Astrid; Höltje, Markus; Adolf, Andrej; Oms, Elisabeth; Hagemann, Sandra; Ahnert-Hilger, Gudrun; Just, Ingo

    2017-10-27

    The Rho ADP-ribosylating C3 exoenzyme (C3bot) is a bacterial protein toxin devoid of a cell-binding or -translocation domain. Nevertheless, C3 can efficiently enter intact cells, including neurons, but the mechanism of C3 binding and uptake is not yet understood. Previously, we identified the intermediate filament vimentin as an extracellular membranous interaction partner of C3. However, uptake of C3 into cells still occurs (although reduced) in the absence of vimentin, indicating involvement of an additional host cell receptor. C3 harbors an Arg-Gly-Asp (RGD) motif, which is the major integrin-binding site, present in a variety of integrin ligands. To check whether the RGD motif of C3 is involved in binding to cells, we performed a competition assay with C3 and RGD peptide or with a monoclonal antibody binding to β1-integrin subunit and binding assays in different cell lines, primary neurons, and synaptosomes with C3-RGD mutants. Here, we report that preincubation of cells with the GRGDNP peptide strongly reduced C3 binding to cells. Moreover, mutation of the RGD motif reduced C3 binding to intact cells and also to recombinant vimentin. Anti-integrin antibodies also lowered the C3 binding to cells. Our results indicate that the RGD motif of C3 is at least one essential C3 motif for binding to host cells and that integrin is an additional receptor for C3 besides vimentin. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  20. Clinical effects and antivenom dosing in brown snake (Pseudonaja spp. envenoming--Australian snakebite project (ASP-14.

    Directory of Open Access Journals (Sweden)

    George E Allen

    Full Text Available BACKGROUND: Snakebite is a global health issue and treatment with antivenom continues to be problematic. Brown snakes (genus Pseudonaja are the most medically important group of Australian snakes and there is controversy over the dose of brown snake antivenom. We aimed to investigate the clinical and laboratory features of definite brown snake (Pseudonaja spp. envenoming, and determine the dose of antivenom required. METHODS AND FINDING: This was a prospective observational study of definite brown snake envenoming from the Australian Snakebite Project (ASP based on snake identification or specific enzyme immunoassay for Pseudonaja venom. From January 2004 to January 2012 there were 149 definite brown snake bites [median age 42 y (2-81 y; 100 males]. Systemic envenoming occurred in 136 (88% cases. All envenomed patients developed venom induced consumption coagulopathy (VICC, with complete VICC in 109 (80% and partial VICC in 27 (20%. Systemic symptoms occurred in 61 (45% and mild neurotoxicity in 2 (1%. Myotoxicity did not occur. Severe envenoming occurred in 51 patients (38% and was characterised by collapse or hypotension (37, thrombotic microangiopathy (15, major haemorrhage (5, cardiac arrest (7 and death (6. The median peak venom concentration in 118 envenomed patients was 1.6 ng/mL (Range: 0.15-210 ng/mL. The median initial antivenom dose was 2 vials (Range: 1-40 in 128 patients receiving antivenom. There was no difference in INR recovery or clinical outcome between patients receiving one or more than one vial of antivenom. Free venom was not detected in 112/115 patients post-antivenom with only low concentrations (0.4 to 0.9 ng/ml in three patients. CONCLUSIONS: Envenoming by brown snakes causes VICC and over a third of patients had serious complications including major haemorrhage, collapse and microangiopathy. The results of this study support accumulating evidence that giving more than one vial of antivenom is unnecessary in brown snake

  1. Amnesic Shellfish Poisoning (ASP)

    DEFF Research Database (Denmark)

    Ravn, H.

    HAB Publ. Ser. vol 1 is a supplement to Chapter 7 Mehtods for Domoic Acid, the Amnesic Shellfish Poisons in the IOC Manual of Harmful Marine Microalgae......HAB Publ. Ser. vol 1 is a supplement to Chapter 7 Mehtods for Domoic Acid, the Amnesic Shellfish Poisons in the IOC Manual of Harmful Marine Microalgae...

  2. Resistance mutations of Pro197, Asp376 and Trp574 in the acetohydroxyacid synthase (AHAS) affect pigments, growths, and competitiveness of Descurainia sophia L.

    Science.gov (United States)

    Zhang, Yongzhi; Xu, Yufang; Wang, Shipeng; Li, Xuefeng; Zheng, Mingqi

    2017-11-27

    D. Sophia is one of the most problematic weed species infesting winter wheat in China, and has evolved high resistance to tribenuron-methyl. Amino acid substitutions at site of Pro197, Asp376 and Trp574 in acetohydroxyacid synthase (AHAS) were mainly responsible for D. sophia resistance to tribenuron-methyl. In this study, D. sophia plant individually homozygous for specific AHAS mutation (Pro197Leu, Pro197His, Pro197Ser, Pro197Thr, Asp376Glu and Trp574Leu) were generated. In addition, the effects of resistance mutations on pigments, growths and competitiveness of susceptible (S) and resistant (R) plants of D. sophia were investigated. The results indicated the R plants carrying Pro197Leu or Pro197His or Asp376Glu or Trp574Leu displayed stronger competitiveness than S plants. The adverse effects on R plants aggravated with the increase of R plants proportion, which made the R plants against domination the weed community in absent of herbicide selection. Therefore, these resistance mutation have no obvious adverse effects on the pigments (chlorophyll a, chlorophyll b and carotenoid), relative growth rates (RGR), leaf area ratio (LAR) and net assimilation rate (NAR) of R plants.

  3. Differential effect of beetroot bread on postprandial DBP according to Glu298Asp polymorphism in the eNOS gene: a pilot study.

    Science.gov (United States)

    Hobbs, D A; George, T W; Lovegrove, J A

    2014-12-01

    Our objective was to investigate whether the presence of Glu298Asp polymorphism in the endothelial NO synthase (eNOS) gene differentially affects the postprandial blood pressure response to dietary nitrate-rich beetroot bread. A randomised, single-blind, controlled, crossover acute pilot study was performed in 14 healthy men (mean age: 34±9 years) who were retrospectively genotyped for Glu298Asp polymorphism (7GG; T carriers 7). Volunteers were randomised to receive 200 g beetroot-enriched bread (1.1 mmol nitrate) or control bread (no beetroot; 0.01 mmol nitrate) on two separate occasions 10 days apart. Baseline and incremental area under the curve of blood pressure and NOx (nitrate/nitrite) were measured for a 6-h postprandial period. A treatment × genotype interaction was observed for diastolic blood pressure (Pconsumption of beetroot bread compared with control bread. No significant differences were observed in the GG group. The beneficial diastolic blood pressure reduction was observed only in the T carriers of the Glu298Asp polymorphism in the eNOS gene after consumption of nitrate-rich beetroot bread. These data require confirmation in a larger population group.

  4. An ecological vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment: system development, treatment performance, and mathematical modeling.

    Science.gov (United States)

    Yuan, Jiajia; Dong, Wenyi; Sun, Feiyun; Li, Pu; Zhao, Ke

    2016-05-01

    An environment-friendly decentralized wastewater treatment process that is comprised of activated sludge process (ASP) and wetland vegetation, named as vegetation-activated sludge process (V-ASP), was developed for decentralized wastewater treatment. The long-term experimental results evidenced that the vegetation sequencing batch reactor (V-SBR) process had consistently stable higher removal efficiencies of organic substances and nutrients from domestic wastewater compared with traditional sequencing batch reactor (SBR). The vegetation allocated into V-SBR system could not only remove nutrients through its vegetation transpiration ratio but also provide great surface area for microorganism activity enhancement. This high vegetation transpiration ratio enhanced nutrients removal effectiveness from wastewater mainly by flux enhancement, oxygen and substrate transportation acceleration, and vegetation respiration stimulation. A mathematical model based on ASM2d was successfully established by involving the specific function of vegetation to simulate system performance. The simulation results on the influence of operational parameters on V-ASP treatment effectiveness demonstrated that V-SBR had a high resistance to seasonal temperature fluctuations and influent loading shocking.

  5. Flexible xxx-asp/asn and gly-xxx residues of equine cytochrome C in matrix-assisted laser desorption/ionization in-source decay mass spectrometry.

    Science.gov (United States)

    Takayama, Mitsuo

    2012-01-01

    The backbone flexibility of a protein has been studied from the standpoint of the susceptibility of amino acid residues to in-source decay (ISD) in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Residues more susceptible to MALDI-ISD, namely Xxx-Asp/Asn and Gly-Xxx, were identified from the discontinuous intense peak of c'-ions originating from specific cleavage at N-Cα bonds of the backbone of equine cytochrome c. The identity of the residues susceptible to ISD was consistent with the known flexible backbone amides as estimated by hydrogen/deuterium exchange (HDX) experiments. The identity of these flexible amino acid residues (Asp, Asn, and Gly) is consistent with the fact that these residues are preferred in flexible secondary structure free from intramolecular hydrogen-bonded structures such as α-helix and β-sheet. The MALDI-ISD spectrum of equine cytochrome c gave not only intense N-terminal side c'-ions originating from N-Cα bond cleavage at Xxx-Asp/Asn and Gly-Xxx residues, but also C-terminal side complement z'-ions originating from the same cleavage sites. The present study implies that MALDI-ISD can give information about backbone flexibility of proteins, comparable with the protection factors estimated by HDX.

  6. Flexible Xxx–Asp/Asn and Gly–Xxx Residues of Equine Cytochrome c in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry

    Science.gov (United States)

    Takayama, Mitsuo

    2012-01-01

    The backbone flexibility of a protein has been studied from the standpoint of the susceptibility of amino acid residues to in-source decay (ISD) in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Residues more susceptible to MALDI-ISD, namely Xxx–Asp/Asn and Gly–Xxx, were identified from the discontinuous intense peak of c′-ions originating from specific cleavage at N–Cα bonds of the backbone of equine cytochrome c. The identity of the residues susceptible to ISD was consistent with the known flexible backbone amides as estimated by hydrogen/deuterium exchange (HDX) experiments. The identity of these flexible amino acid residues (Asp, Asn, and Gly) is consistent with the fact that these residues are preferred in flexible secondary structure free from intramolecular hydrogen-bonded structures such as α-helix and β-sheet. The MALDI-ISD spectrum of equine cytochrome c gave not only intense N-terminal side c′-ions originating from N–Cα bond cleavage at Xxx–Asp/Asn and Gly–Xxx residues, but also C-terminal side complement z′-ions originating from the same cleavage sites. The present study implies that MALDI-ISD can give information about backbone flexibility of proteins, comparable with the protection factors estimated by HDX. PMID:24349908

  7. Toll receptor 4 Asp299Gly polymorphism and its association with preterm birth and premature rupture of membranes in a South American population.

    Science.gov (United States)

    Rey, G; Skowronek, F; Alciaturi, J; Alonso, J; Bertoni, B; Sapiro, R

    2008-09-01

    Preterm birth (PTB) is a worldwide health problem and remains the leading cause of perinatal morbidity and mortality. Systemic and local intrauterine infections have been implicated in the pathogenesis of preterm labor and delivery. Common pathways between PTB, premature rupture of ovular membranes (PROM) and altered molecular routes of inflammation have been proposed. There is evidence to support a genetic component in these conditions. Lipopolysaccharide (LPS), a component of the cell wall of Gram-negative bacteria, is thought to play a key role in eliciting an inflammatory response. LPS is recognized by proteins of the innate immune system, including Toll-like receptor 4 (TLR4). Individuals from some European countries carrying the variant alleles resulting in an amino acid substitution (Asp299Gly) are at increased risk of Gram-negative infections and premature birth. The objective of this study was to determine if preterm newborns have different allele frequency of the Asp299Gly TLR4 variant from healthy term neonates in Uruguay. The impact of PROM was also examined. There was an increase in the risk for fetuses carrying the Asp299Gly substitution in TLR4 of being severely premature (<33 weeks) and to present PROM at the same time.

  8. Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.

    Science.gov (United States)

    Naito, Yoshiki; Tomita, Motonori

    2013-08-01

    The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a 1 bp substitution in exon 1 (Jukkoku-type GA20ox-2 mutant allele: Jukkoku_GA20ox-2). An allele-specific primer (ASP)-polymerase chain reaction (PCR) with primers SD1F3 and SD1JR gave a PCR product specific to Jukkoku_GA20ox-2. In addition, ASP-PCR with primers SD1F3 and SD1NRM (which contains a mismatch at the third nucleotide from the 3'-terminus of SD1NR) gave a PCR product specific to non-Jukkoku_GA20ox-2. Multiplex ASP-PCR using SD1F3, VIC dye-labeled SD1JR, and FAM dye-labeled SD1NRM enabled simultaneous codominant detection of Jukkoku_GA20ox-2 and non-Jukkoku_GA20ox-2 among 188 cultivars. Also, Hikarishinseiki is identifiable by RM253 polymorphism from 11 cultivars carrying Jukkoku_GA20ox-2. Taken together, our results establish a methodology for distinguishing Hikarishinseiki.

  9. Caprine PrP variants harboring Asp-146, His-154 and Gln-211 alleles display reduced convertibility upon interaction with pathogenic murine prion protein in scrapie infected cells.

    Science.gov (United States)

    Kanata, Eirini; Arsenakis, Minas; Sklaviadis, Theodoros

    2016-09-02

    Scrapie, the prion disease of sheep and goats, is a devastating malady of small ruminants. Due to its infectious nature, epidemic outbreaks may occur in flocks/herds consisting of highly susceptible animals. Field studies identified scrapie-protective caprine PrP variants, harboring specific single amino acid changes (Met-142, Arg-143, Asp-146, Ser-146, His-154, Gln-211 and Lys-222). Their effects are under further evaluation, and aim to determine the most protective allele. We assessed some of these variants (Asp-146, His-154, Gln-211 and Lys-222), after their exogenous expression as murine-caprine chimeras in a scrapie- infected murine cell line. We report that exogenously expressed PrPs undergo conformational conversion upon interaction with the endogenous pathological murine prion protein (PrP SC ), which results in the detection of goat-specific and partially PK-resistant moieties. These moieties display a PK-resistance pattern distinct from the one detected in natural goat scrapie cases. Within this cellular model, distinct conformational conversion potentials were assigned to the tested variants. Molecules carrying the Asp-146, His-154 and Gln-211 alleles showed significantly lower conversion levels compared to wild type, confirming their protective effects against scrapie. Although we utilized a heterologous conversion system, this is to our knowledge, the first study of caprine PrP variants in a cellular context of scrapie, that confirms the protective effects of some of the studied alleles.

  10. A novel Asp121Asn mutation of myelin protein zero is associated with late-onset axonal Charcot-Marie-Tooth disease, hearing loss and pupil abnormalities

    Directory of Open Access Journals (Sweden)

    Xiaohui Duan

    2016-09-01

    Full Text Available Myelin protein zero (MPZ is a major component of compact myelin in peripheral nerves. Mutations in MPZ have been associated with different Charcot–Marie–Tooth disease (CMT phenotypes (CMT1B, CMT2I/J, CMTDI, Dejerine–Sottas syndrome, and congenital hypomyelination neuropathy. Here, we report phenotypic variability in a four-generation Chinese family with the MPZ mutation Asp121Asn. Genetic testing was performed on 9 family members and 200 controls. Clinical, electrophysiological and skeletal muscle MRI assessments were available for review in 6 family members. A novel heterozygous missense mutation, Asp121Asn, was observed in 5 affected members of the family. Unaffected relatives and 200 normal controls were without the mutation. Four of the affected members of the family displayed late-onset, predominantly axonal sensory and motor neuropathy, pupil abnormalities, and progressive sensorineural hearing loss. One young affected member presented with Argyll–Robertson pupils and diminished deep tendon reflexes in the lower limbs. The MPZ mutation Asp121Asn may be associated with late-onset axonal neuropathy, early-onset hearing loss and pupil abnormalities. Our report expands the number and phenotypic spectrum of MPZ mutations.

  11. New Waveform Analysis Method: the Chirplet Transform. Characterisation of the Induced-Seismicity From the Lacq gas Field (Western Pyrenees, France).

    Science.gov (United States)

    Bardainne, T.; Gaillot, P.; Senechal, G.

    2004-12-01

    Whole waveform records of waves radiated by induced-seismic events contain considerable information on event location, propagation medium, failure type, source size, and state of stress. Modern seismological studies of induced-seismicity related with hydrocarbons exploitation or storage benefit from time-frequency (TF) analysis techniques such as the windowed Fourier transform or the wavelet transform. These TF representations with fixed windows or kernels perform well only for limited classes of seismograms. Representations with signal-dependent kernels can overcome this limitation. A new five-parameter atomic decomposition of chirplets is developed for compact and precise representation of seismograms with chirp components. The five-parameter chirplet atom is obtained from the unit Gaussian function by successive applications of scaling, fractional Fourier transform, time-shift and frequency-shift operators of various duration. So, the chirplet transform unifies many of the disparate signal representation methods. In particular, the wide-range of TF methods such as the Fourier transform, spectrogram, Wigner distribution, and wavelet transform may each be shown to be a special case of the chirplet transform. We developed a methodology that recognizes the attributes of the waveforms of each seismic events. We first present and illustrate on synthetic examples this new formalism. Next, we illustrate its potentialities on seismograms recorded by 17 seismometers (Mark-Product L-4C - 12 bits) during the monitoring period (1974-1997) of the Lacq gas field (Western Pyrenees, France). Using the chirplet transform, each wave packet of the seismogram (mostly single component, sampling frequency = 150 Hz, duration = 15 s) is decomposed in terms of energy, arrival time, frequency, duration, shape (time-shear) and frequency modulation (frequency-shear). Thus, selected seismograms (N ˜ 5500 for 592 well-localized events) are described by a set of sextuplets which provide

  12. Crustal-scale alpine tectonic evolution of the western Pyrenees - eastern Cantabrian Mountains (N Spain) from integration of structural data, low-T thermochronology and seismic constraint

    Science.gov (United States)

    DeFelipe, I.; Pedreira, D.; Pulgar, J. A.; Van der Beek, P.; Bernet, M.; Pik, R.

    2017-12-01

    The Pyrenean-Cantabrian Mountain belt extends in an E-W direction along the northern border of Spain and resulted from the convergence between the Iberian and European plates from the Late Cretaceous to the Miocene, in the context of the Alpine orogeny. The main aim of this work is to characterize the tectonic evolution at a crustal-scale of the transition zone from the Pyrenees to the Cantabrian Mountains, in the eastern Basque-Cantabrian Basin (BCB). We integrate structural work, thermochronology (apatite fission track and zircon (U-Th)/He) and geophysical information (shallow seismic reflection profiles, deep seismic refraction/wide-angle reflection profiles and seismicity distribution) to propose an evolutionary model since the Jurassic to the present. During the Albian, hyperextension related to the opening of the Bay of Biscay yielded to mantle unroofing to the base of the BCB. This process was favored by a detachment fault that connected the mantle in its footwall with the base of a deep basin in its hanging wall. During this process, the basin experienced HT metamorphism and fluid circulation caused the serpentinization of the upper part of the mantle. There is no evidence of seafloor mantle exhumation before the onset of the Alpine orogeny. The thermochronological study points to a N-vergent phase of contractional deformation in the late Eocene represented by the thin-skinned Leiza fault system followed in the early Oligocene by the S-vergent, thick-skinned, Ollín thrust. Exhumation rates for the late Eocene-early Oligocene are of 0.2-0.7 km/Myr. After that period, deformation continues southwards until the Miocene. The crustal-scale structure resultant of the Alpine orogeny consists of an Iberian plate that subducts below the European plate. The crust is segmented into four blocks separated by three S-vergent crustal faults inherited from the Cretaceous extensional period. The P-wave velocities in this transect show anomalous values (7.4 km/s) in the

  13. Hydrogeochemical assessment of groundwater in Kashmir Valley ...

    Indian Academy of Sciences (India)

    Groundwater samples ( = 163) were collected across Kashmir Valley in 2010 to assess the hydrogeochemistry of the groundwater in shallow and deep aquifers and its suitability for domestic, agriculture, horticulture, and livestock purposes. The groundwater is generally alkaline in nature. The electrical conductivity (EC) ...

  14. Potential hydrologic characterization wells in Amargosa Valley

    International Nuclear Information System (INIS)

    Lyles, B.; Mihevc, T.

    1994-09-01

    More than 500 domestic, agricultural, and monitoring wells were identified in the Amargosa Valley. From this list, 80 wells were identified as potential hydrologic characterization wells, in support of the US Department of Energy (DOE) Underground Test Area/Remedial Investigation and Feasibility Study (UGTA/RIFS). Previous hydrogeologic studies have shown that groundwater flow in the basin is complex and that aquifers may have little lateral continuity. Wells located more than 10 km or so from the Nevada Test Site (NTS) boundary may yield data that are difficult to correlate to sources from the NTS. Also, monitoring well locations should be chosen within the guidelines of a hydrologic conceptual model and monitoring plan. Since these do not exist at this time, recompletion recommendations will be restricted to wells relatively close (approximately 20 km) to the NTS boundary. Recompletion recommendations were made for two abandoned agricultural irrigation wells near the town of Amargosa Valley (previously Lathrop Wells), for two abandoned wildcat oil wells about 10 km southwest of Amargosa Valley, and for Test Well 5 (TW-5), about 10 km east of Amargosa Valley

  15. Magnetic Valley: A Knowledge Transfer Project

    Directory of Open Access Journals (Sweden)

    F Humbled

    2011-07-01

    Full Text Available The knowledge transfer project called "Magnetic Valley" that was launched in 2009 is presented below. This project is funded by the Belgian government to investigate and develop products and services that will improve the socio-economic development in the area around the "Centre de Physique du Globe de l'IRM".

  16. Rift Valley fever, Mayotte, 2007-2008.

    Science.gov (United States)

    Sissoko, Daouda; Giry, Claude; Gabrie, Philippe; Tarantola, Arnaud; Pettinelli, François; Collet, Louis; D'Ortenzio, Eric; Renault, Philippe; Pierre, Vincent

    2009-04-01

    After the 2006-2007 epidemic wave of Rift Valley fever (RVF) in East Africa and its circulation in the Comoros, laboratory case-finding of RVF was conducted in Mayotte from September 2007 through May 2008. Ten recent human RVF cases were detected, which confirms the indigenous transmission of RFV virus in Mayotte.

  17. Hydrogeochemical assessment of groundwater in Kashmir Valley ...

    Indian Academy of Sciences (India)

    Groundwater samples (n = 163) were collected across Kashmir Valley in 2010 to assess the hydrogeo- chemistry of the groundwater in shallow and deep aquifers and its suitability for domestic, agriculture, horticulture, and livestock purposes. The groundwater is generally alkaline in nature. The electrical conductivity (EC) ...

  18. 27 CFR 9.46 - Livermore Valley.

    Science.gov (United States)

    2010-04-01

    ...) Clayton, CA (1953; Photorevised 1980; Minor Revision 1994); (2) Diablo, Calif. (1953; Photorevised 1980... Livermore Valley viticultural area's boundary is defined as follows: (1) The beginning point is on the Clayton map at the peak of Mount Diablo (VABM 3849) where the Mount Diablo Base Line and Mount Diablo...

  19. Groundwater links between Kenyan Rift Valley lakes

    OpenAIRE

    Becht, Robert; Mwango, Fred; Muno, Fred Amstrong

    2006-01-01

    The series of lakes in the bottom of the Kenyan Rift valley are fed by rivers and springs. Based on the water balance, the relative positions determining the regional groundwater flow systems and the analysis of natural isotopes it can be shown that groundwater flows from lake Naivasha to lake Magadi, Elementeita, Nakuru and Bogoria.

  20. Preceramic occupations in the orinoco river valley.

    Science.gov (United States)

    Barse, W P

    1990-12-07

    Two sites in the Orinoco Valley containing preceramic from excavated contexts are described. Radiocarbon dating and stylistic comparisons indicate that the northern tropical lowlands were inhabited at the onset of the Holocene, suggesting a time depth of 9000 years before the present for tropical forest-savanna adaptations in northern South America.

  1. Reemergence of Rift Valley fever, Mauritania, 2010.

    Science.gov (United States)

    Faye, Ousmane; Ba, Hampathé; Ba, Yamar; Freire, Caio C M; Faye, Oumar; Ndiaye, Oumar; Elgady, Isselmou O; Zanotto, Paolo M A; Diallo, Mawlouth; Sall, Amadou A

    2014-02-01

    A Rift Valley fever (RVF) outbreak in humans and animals occurred in Mauritania in 2010. Thirty cases of RVF in humans and 3 deaths were identified. RVFV isolates were recovered from humans, camels, sheep, goats, and Culex antennatus mosquitoes. Phylogenetic analysis of isolates indicated a virus origin from western Africa.

  2. Unexpected Rift Valley fever outbreak, northern Mauritania.

    Science.gov (United States)

    El Mamy, Ahmed B O; Baba, Mohamed Ould; Barry, Yahya; Isselmou, Katia; Dia, Mamadou L; El Kory, Mohamed O B; Diop, Mariam; Lo, Modou Moustapha; Thiongane, Yaya; Bengoumi, Mohammed; Puech, Lilian; Plee, Ludovic; Claes, Filip; de La Rocque, Stephane; Doumbia, Baba

    2011-10-01

    During September-October 2010, an unprecedented outbreak of Rift Valley fever was reported in the northern Sahelian region of Mauritania after exceptionally heavy rainfall. Camels probably played a central role in the local amplification of the virus. We describe the main clinical signs (hemorrhagic fever, icterus, and nervous symptoms) observed during the outbreak.

  3. Rift Valley fever: A neglected zoonotic disease?

    Science.gov (United States)

    Rift Valley fever (RVF) is a serious viral disease of animals and humans in Africa and the Middle East that is transmitted by mosquitoes. First isolated in Kenya during an outbreak in 1930, subsequent outbreaks have had a significant impact on animal and human health, as well as national economies. ...

  4. Geomorphological hazards in Swat valley, Pakistan

    International Nuclear Information System (INIS)

    Usman, A.

    1999-01-01

    This study attempts to describe, interpret and analyze, in depth, the varied geomorphological hazards and their impacts prevailing in the swat valley locate in the northern hilly and mountainous regions of Pakistan. The hills and mountains re zones of high geomorphological activity with rapid rates of weathering, active tectonic activities, abundant precipitation, rapid runoff and heavy sediment transport. Due to the varied topography, lithology, steep slope, erodible soil, heavy winter snowfall and intensive rainfall in the spring and summer seasons, several kinds of geomorphological hazards, such as geomorphic gravitational hazards, Fluvial hazards, Glacial hazards, Geo tectonic hazards, are occurring frequently in swat valley. Amongst them, geomorphic gravitational hazards, such as rock fall rock slide, debris slide mud flow avalanches, are major hazards in mountains and hills while fluvial hazards and sedimentation are mainly confined to the alluvial plain and lowlands of the valley. The Getechtonic hazards, on the other hand, have wide spread distribution in the valley the magnitude and occurrence of each king of hazard is thus, varied according to intensity of process and physical geographic environment. This paper discusses the type distribution and damage due to the various geomorphological hazards and their reduction treatments. The study would to be of particular importance and interest to both natural and social scientists, as well as planner, environmentalists and decision-makers for successful developmental interventions in the region. (author)

  5. Rift Valley Fever, Mayotte, 2007–2008

    Science.gov (United States)

    Giry, Claude; Gabrie, Philippe; Tarantola, Arnaud; Pettinelli, François; Collet, Louis; D’Ortenzio, Eric; Renault, Philippe; Pierre, Vincent

    2009-01-01

    After the 2006–2007 epidemic wave of Rift Valley fever (RVF) in East Africa and its circulation in the Comoros, laboratory case-finding of RVF was conducted in Mayotte from September 2007 through May 2008. Ten recent human RVF cases were detected, which confirms the indigenous transmission of RFV virus in Mayotte. PMID:19331733

  6. Business plan Hatchery Facility Zambezi Valley, Mozambique

    NARCIS (Netherlands)

    Vernooij, A.G.; Wilschut, S.

    2015-01-01

    This business plan focuses on the establishment of a hatchery, one of the essential elements of a sustainable and profitable poultry meat value chain. There is a growing demand for poultry meat in the Zambezi Valley, and currently a large part of the consumed broilers comes from other parts of the

  7. Babesiosis in Lower Hudson Valley, New York

    Centers for Disease Control (CDC) Podcasts

    2011-05-12

    This podcast discusses a study about an increase in babesiosis in the Lower Hudson Valley of New York state. Dr. Julie Joseph, Assistant Professor of Medicine at New York Medical College, shares details of this study.  Created: 5/12/2011 by National Center for Emerging Zoonotic and Infectious Diseases (NCEZID).   Date Released: 5/23/2011.

  8. Pumpernickel Valley Geothermal Project Thermal Gradient Wells

    Energy Technology Data Exchange (ETDEWEB)

    Z. Adam Szybinski

    2006-01-01

    The Pumpernickel Valley geothermal project area is located near the eastern edge of the Sonoma Range and is positioned within the structurally complex Winnemucca fold and thrust belt of north-central Nevada. A series of approximately north-northeast-striking faults related to the Basin and Range tectonics are superimposed on the earlier structures within the project area, and are responsible for the final overall geometry and distribution of the pre-existing structural features on the property. Two of these faults, the Pumpernickel Valley fault and Edna Mountain fault, are range-bounding and display numerous characteristics typical of strike-slip fault systems. These characteristics, when combined with geophysical data from Shore (2005), indicate the presence of a pull-apart basin, formed within the releasing bend of the Pumpernickel Valley – Edna Mountain fault system. A substantial body of evidence exists, in the form of available geothermal, geological and geophysical information, to suggest that the property and the pull-apart basin host a structurally controlled, extensive geothermal field. The most evident manifestations of the geothermal activity in the valley are two areas with hot springs, seepages, and wet ground/vegetation anomalies near the Pumpernickel Valley fault, which indicate that the fault focuses the fluid up-flow. There has not been any geothermal production from the Pumpernickel Valley area, but it was the focus of a limited exploration effort by Magma Power Company. In 1974, the company drilled one exploration/temperature gradient borehole east of the Pumpernickel Valley fault and recorded a thermal gradient of 160oC/km. The 1982 temperature data from five unrelated mineral exploration holes to the north of the Magma well indicated geothermal gradients in a range from 66 to 249oC/km for wells west of the fault, and ~283oC/km in a well next to the fault. In 2005, Nevada Geothermal Power Company drilled four geothermal gradient wells, PVTG-1

  9. Poultry Slaughter facility Zambezi Valley, Mozambique

    NARCIS (Netherlands)

    Vernooij, A.G.; Wilschut, S.

    2015-01-01

    This business plan focuses on the establishment of a slaughterhouse, one of the essential elements of a sustainable and profitable poultry meat value chain. There is a growing demand for poultry meat in the Zambezi Valley, and currently a large part of the consumed broilers comes from other parts of

  10. A valley-filtering switch based on strained graphene.

    Science.gov (United States)

    Zhai, Feng; Ma, Yanling; Zhang, Ying-Tao

    2011-09-28

    We investigate valley-dependent transport through a graphene sheet modulated by both the substrate strain and the fringe field of two parallel ferromagnetic metal (FM) stripes. When the magnetizations of the two FM stripes are switched from the parallel to the antiparallel alignment, the total conductance, valley polarization and valley conductance excess change greatly over a wide range of Fermi energy, which results from the dependence of the valley-related transmission suppression on the polarity configuration of inhomogeneous magnetic fields. Thus the proposed structure exhibits the significant features of a valley-filtering switch and a magnetoresistance device.

  11. A valley-filtering switch based on strained graphene

    International Nuclear Information System (INIS)

    Zhai Feng; Ma Yanling; Zhang Yingtao

    2011-01-01

    We investigate valley-dependent transport through a graphene sheet modulated by both the substrate strain and the fringe field of two parallel ferromagnetic metal (FM) stripes. When the magnetizations of the two FM stripes are switched from the parallel to the antiparallel alignment, the total conductance, valley polarization and valley conductance excess change greatly over a wide range of Fermi energy, which results from the dependence of the valley-related transmission suppression on the polarity configuration of inhomogeneous magnetic fields. Thus the proposed structure exhibits the significant features of a valley-filtering switch and a magnetoresistance device. (paper)

  12. Valley-selective optical Stark effect in monolayer WS2

    Science.gov (United States)

    Gedik, Nuh

    Monolayer semiconducting transition-metal dichalcogenides (TMDs) have a pair of valleys that, by time-reversal symmetry, are energetically degenerate. Lifting the valley degeneracy in these materials is of great interest because it would allow for valley specific band engineering and offer additional control in valleytronic applications. In this talk, I will show that circularly polarized light, which breaks time-reversal symmetry, can be used to lift the valley degeneracy by means of the optical Stark effect. We demonstrate that this effect is capable of raising the exciton level in monolayer TMD WS2 by as much as 18 meV in a controllable valley-selective manner. The resulting energy shift is extremely large, comparable to the shift that would be obtained using a very high magnetic field (approximately 100 Tesla). These results offer a novel way to control valley degree of freedom, and may provide a means to realize new valley-selective Floquet topological state of matter.

  13. Groundwater quality in the Antelope Valley, California

    Science.gov (United States)

    Dawson, Barbara J. Milby; Belitz, Kenneth

    2012-01-01

    Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Antelope Valley is one of the study areas being evaluated. The Antelope study area is approximately 1,600 square miles (4,144 square kilometers) and includes the Antelope Valley groundwater basin (California Department of Water Resources, 2003). Antelope Valley has an arid climate and is part of the Mojave Desert. Average annual rainfall is about 6 inches (15 centimeters). The study area has internal drainage, with runoff from the surrounding mountains draining towards dry lakebeds in the lower parts of the valley. Land use in the study area is approximately 68 percent (%) natural (mostly shrubland and grassland), 24% agricultural, and 8% urban. The primary crops are pasture and hay. The largest urban areas are the cities of Palmdale and Lancaster (2010 populations of 152,000 and 156,000, respectively). Groundwater in this basin is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay derived from surrounding mountains. The primary aquifers in Antelope Valley are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database. Public-supply wells in Antelope Valley are completed to depths between 360 and 700 feet (110 to 213 meters), consist of solid casing from the land surface to a depth of 180 to 350 feet (55 to 107 meters), and are screened or perforated below the solid casing. Recharge to the groundwater system is primarily runoff from the surrounding mountains, and by direct infiltration of irrigation and sewer and septic

  14. Groundwater quality in the Owens Valley, California

    Science.gov (United States)

    Dawson, Barbara J. Milby; Belitz, Kenneth

    2012-01-01

    Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Owens Valley is one of the study areas being evaluated. The Owens study area is approximately 1,030 square miles (2,668 square kilometers) and includes the Owens Valley groundwater basin (California Department of Water Resources, 2003). Owens Valley has a semiarid to arid climate, with average annual rainfall of about 6 inches (15 centimeters). The study area has internal drainage, with runoff primarily from the Sierra Nevada draining east to the Owens River, which flows south to Owens Lake dry lakebed at the southern end of the valley. Beginning in the early 1900s, the City of Los Angeles began diverting the flow of the Owens River to the Los Angeles Aqueduct, resulting in the evaporation of Owens Lake and the formation of the current Owens Lake dry lakebed. Land use in the study area is approximately 94 percent (%) natural, 5% agricultural, and 1% urban. The primary natural land cover is shrubland. The largest urban area is the city of Bishop (2010 population of 4,000). Groundwater in this basin is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay derived from surrounding mountains. Recharge to the groundwater system is primarily runoff from the Sierra Nevada, and by direct infiltration of irrigation. The primary sources of discharge are pumping wells, evapotranspiration, and underflow to the Owens Lake dry lakebed. The primary aquifers in Owens Valley are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database

  15. Microscopic Identification of Prokaryotes in Modern and Ancient Halite, Saline Valley and Death Valley, California

    Science.gov (United States)

    Schubert, Brian A.; Lowenstein, Tim K.; Timofeeff, Michael N.

    2009-06-01

    Primary fluid inclusions in halite crystallized in Saline Valley, California, in 1980, 2004-2005, and 2007, contain rod- and coccoid-shaped microparticles the same size and morphology as archaea and bacteria living in modern brines. Primary fluid inclusions from a well-dated (0-100,000 years), 90 m long salt core from Badwater Basin, Death Valley, California, also contain microparticles, here interpreted as halophilic and halotolerant prokaryotes. Prokaryotes are distinguished from crystals on the basis of morphology, optical properties (birefringence), and uniformity of size. Electron micrographs of microparticles from filtered modern brine (Saline Valley), dissolved modern halite crystals (Saline Valley), and dissolved ancient halite crystals (Death Valley) support in situ microscopic observations that prokaryotes are present in fluid inclusions in ancient halite. In the Death Valley salt core, prokaryotes in fluid inclusions occur almost exclusively in halite precipitated in perennial saline lakes 10,000 to 35,000 years ago. This suggests that trapping and preservation of prokaryotes in fluid inclusions is influenced by the surface environment in which the halite originally precipitated. In all cases, prokaryotes in fluid inclusions in halite from the Death Valley salt core are miniaturized (indigenous to the halite and starvation survival may be the normal response of some prokaryotes to entrapment in fluid inclusions for millennia. These results reinforce the view that fluid inclusions in halite and possibly other evaporites are important repositories of microbial life and should be carefully examined in the search for ancient microorganisms on Earth, Mars, and elsewhere in the Solar System.

  16. Valley Topological Phases in Bilayer Sonic Crystals

    Science.gov (United States)

    Lu, Jiuyang; Qiu, Chunyin; Deng, Weiyin; Huang, Xueqin; Li, Feng; Zhang, Fan; Chen, Shuqi; Liu, Zhengyou

    2018-03-01

    Recently, the topological physics in artificial crystals for classical waves has become an emerging research area. In this Letter, we propose a unique bilayer design of sonic crystals that are constructed by two layers of coupled hexagonal array of triangular scatterers. Assisted by the additional layer degree of freedom, a rich topological phase diagram is achieved by simply rotating scatterers in both layers. Under a unified theoretical framework, two kinds of valley-projected topological acoustic insulators are distinguished analytically, i.e., the layer-mixed and layer-polarized topological valley Hall phases, respectively. The theory is evidently confirmed by our numerical and experimental observations of the nontrivial edge states that propagate along the interfaces separating different topological phases. Various applications such as sound communications in integrated devices can be anticipated by the intriguing acoustic edge states enriched by the layer information.

  17. Valley Fever: Earth Observations for Risk Reduction

    Science.gov (United States)

    Sprigg, W. A.

    2012-12-01

    Advances in satellite Earth observation systems, numerical weather prediction, and dust storm modeling yield new tools for public health warnings, advisories and epidemiology of illnesses associated with airborne desert dust. Valley Fever, endemic from California through the US/Mexico border region into Central and South America, is triggered by inhalation of soil-dwelling fungal spores. The path from fungal growth to airborne threat depends on environmental conditions observable from satellite. And space-based sensors provide initial conditions for dust storm forecasts and baselines for the epidemiology of Valley Fever and other dust-borne aggravation of respiratory and cardiovascular disease. A new Pan-American Center for the World Meteorological Organization Sand and Dust Storm Warning Advisory and Assessment System creates an opportunity to advance Earth science applications in public health.

  18. Eco-Hydrological Modelling of Stream Valleys

    DEFF Research Database (Denmark)

    Johansen, Ole

    Predicting the effects of hydrological alterations on terrestrial stream valley ecosystems requires multidisciplinary approaches involving both engineers and ecologists. Groundwater discharge in stream valleys and other lowland areas support a number of species rich ecosystems, and their protection...... is prioritised worldwide. Protection requires improved knowledge on the functioning of these ecosystems and especially the linkages between vegetation, groundwater discharge and water level conditions are crucial for management applications. Groundwater abstraction affects catchment hydrology and thereby also...... groundwater discharge. Numerical hydrological modelling has been widely used for evaluation of sustainable groundwater resources and effects of abstraction, however, the importance of local scale heterogeneity becomes increasingly important in the assessment of local damage to these groundwater dependent...

  19. Integrated multi-stratigraphic study of the Coll de Terrers late Permian-Early Triassic continental succession from the Catalan Pyrenees (NE Iberian Peninsula): A geologic reference record for equatorial Pangaea

    Science.gov (United States)

    Mujal, Eudald; Fortuny, Josep; Pérez-Cano, Jordi; Dinarès-Turell, Jaume; Ibáñez-Insa, Jordi; Oms, Oriol; Vila, Isabel; Bolet, Arnau; Anadón, Pere

    2017-12-01

    The most severe biotic crisis on Earth history occurred during the Permian-Triassic (PT) transition around 252 Ma. Whereas in the marine realm such extinction event is well-constrained, in terrestrial settings it is still poorly known, mainly due to the lack of suitable complete sections. This is utterly the case along the Western Tethys region, located at Pangaea's equator, where terrestrial successions are typically build-up of red beds often characterised by a significant erosive gap at the base of the Triassic strata. Henceforth, documenting potentially complete terrestrial successions along the PT transition becomes fundamental. Here, we document the exceptional Coll de Terrers area from the Catalan Pyrenees (NE Iberian Peninsula), for which a multidisciplinary research is conducted along the PT transition. The red-bed succession, located in a long E-W extended narrow rift system known as Pyrenean Basin, resulted from a continuous sedimentary deposition evolving from meandering (lower Upper Red Unit) to playa-lake/ephemeral lacustrine (upper Upper Red Unit) and again to meandering settings (Buntsandstein facies). Sedimentary continuity is suggested by preliminary cyclostratigraphic analysis that warrants further analysis. Our combined sedimentological, mineralogical and geochemical data infer a humid-semiarid-humid climatic trend across the studied succession. The uppermost Permian strata, deposited under an orbitally controlled monsoonal regime, yields a relatively diverse ichnoassemblage mainly composed of tetrapod footprints and arthropod trace fossils. Such fossils indicate appropriate life conditions and water presence in levels that also display desiccation structures. These levels alternate with barren intervals formed under dry conditions, being thus indicative of strong seasonality. All these features are correlated with those reported elsewhere in Gondwana and Laurasia, and suggest that the Permian-Triassic boundary might be recorded somewhere around

  20. Asp to Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli

    Science.gov (United States)

    Cheng, Bokun; Annamalai, Thirunavakkarasu; Sorokin, Elena; Abrenica, Maria; Aedo, Sandra; Tse-Dinh, Yuk-Ching

    2010-01-01

    SUMMARY The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved in magnesium ion coordination for catalysis. Medium to high copy number plasmid clones of Yersinia pestis topoisomerase I (YpTOP) with Asp to Asn substitution at the first aspartate residue (D117N) of this motif could not be generated in Escherichia coli without second site mutation even when expression was under the control of the tightly regulated BAD promoter and suppressed by 2% glucose in the medium. Arabinose induction of a single copy YpTOP-D117N mutant gene integrated into the chromosome resulted in ~105 fold of cell killing in 2.5 h. Attempt to induce expression of the corresponding E. coli topoisomerase I mutant (EcTOP-D111N) encoded on a high copy number plasmid resulted in either loss of viability or reversion of the clone to wild-type. High copy plasmid clones of YpTOP-D119N and EcTOP-D113N with the Asn substitution at the second Asp of the TOPRIM motif could be stably maintained, but overexpression also decreased cell viability significantly. The Asp to Asn substitutions at these TOPRIM residues can selectively decrease Mg2+ binding affinity with minimal disruption of the active site geometry, leading to trapping of the covalent complex with cleaved DNA and causing bacterial cell death. The extreme sensitivity of the first TOPRIM position suggested that this might be a useful site for binding of small molecules that could act as topoisomerase poisons. PMID:19013470

  1. Environment of copper in Pseudomonas aeruginosa azurin probed by binding of exogenous ligands to Met121X (X = Gly, Ala, Val, Leu, or Asp) mutants.

    Science.gov (United States)

    Bonander, N; Karlsson, B G; Vänngård, T

    1996-02-20

    The binding of small exogenous ligands to mutants of the blue copper protein azurin from Pseudomonas aeruginosa, altered in the axial position, Met121X (X = Gly, Ala, Val, Leu, or Asp), has been studied with optical and electron paramagnetic resonance (EPR) spectroscopy. The results show that small molecules can enter the pocket left by the side chain of Met121. For azide, the dissociation constants are Leu > Val > Ala, reflecting the increasing space available. The Gly and Asp mutants bind azide less strongly than the Ala mutant, due to competition with water (Gly) and the polar side chain (Asp). Similar trends are found for thiocyanate. Cyanide binds equally well to the Ala and Val mutants. A number of other small potential ligands were tried. Alcohols do not affect room-temperature optical spectra, but at low temperatures, the EPR spectrum is stellacyanin-like, indicative of a weak axial interaction. Ligands binding with a carboxyl group or nitrogen (e.g. acetate or azide) convert the metal center to a form intermediate between regular types 1 and 2, presumably by pulling the copper ion out of the trigonal plane formed by Cys(S) and two His(N). Cyanide interacts strongly as shown by the hyperfine coupling to the 13C nucleus. With increasing strength of the axial interaction, the two major bands in the visible region (600 and 400-500 nm) shift in parallel to higher energy, and at the same time, the strength of the latter transition increases at the expense of the former. This demonstrates that these transitions have a common origin, namely S-to-Cu charge transfer transition.

  2. Historic Settlement in the Upper Tombigbee Valley.

    Science.gov (United States)

    1981-02-28

    SETTLEMENT IN THE UPPER TOMBIGBEE VALLEY ITT By LJU i8 James F. Doster LA- and David C. Weaver dhA dout hc onaproe 82 07 16 063 frPublic relgoise cmd *We...10 1/2 yds plain wht. Bobnut I ps. Black crape $2.50 2.50 2 2/- 2.63 18 doz. buttons 29 1/2, (ea) 5.31 24 yds Bobnut Lace 2 8¢ 1.92 5 pr. Ladies kid

  3. Can Springs Cut Valleys Into Bedrock?

    Science.gov (United States)

    Lamb, M. P.; Dietrich, W. E.

    2004-12-01

    Valleys formed from groundwater sapping are thought to have a characteristic form including steep walls, flat floors, and amphitheatre-like heads. Observations of these features on Earth and Mars have led to the morphologic-based interpretation that groundwater sapping is an important valley forming process. This interpretation has significant implications for Mars in particular because it has been used to constrain Martian hydrology and the associated prospects for life. However, a mechanistic understanding of sapping erosion has only been demonstrated for granular mediums (i.e. sand). Many of the "sapping" valleys on Earth and (likely) Mars have been carved into bedrock, and the extension of previous work to bedrock erosion is unclear. To our knowledge, a process-based understanding of seepage erosion in bedrock does not exist, even though it is thought to be a first order geomorphic process on Earth and Mars. In order to address this knowledge gap, we are currently investigating Box Canyon, Idaho. Box Canyon, developed in the Snake River Plain, has many of the morphologic features often associated with sapping valleys. In addition, it was carved into basaltic bedrock and has a large spring emanating from its amphitheatre-like head, making it an ideal candidate for a sapping origin. There is currently no overland flow contribution to the canyon; however, based on mapping bedrock scours, a paleo-flood from an unknown upslope source did enter the canyon (and perhaps carved it). We present some first order hydraulic measurements, sediment transport calculations, and field observations to try and constrain the types of flows needed to carve Box Canyon. These flows could conceivably be derived from expansion of the current spring. Direct observation at the head of the canyon has not yet indicated how sapping could be responsible for the erosion of the headwall. We are using various dating techniques to constrain the timing and rate of headwall migration to constrain

  4. Hemoglobin J-Baltimore (beta 16(A13)Gly----Asp): interference with the assay of HbA1c.

    Science.gov (United States)

    Vandenesch, F; Baklouti, F; Francina, A; Vianey-Liaud, C; Bertrand, A; Le Dévéhat, C; Delaunay, J

    1987-09-30

    Three independent cases of Hemoglobin J-Baltimore(beta 16(A13)Gly----Asp) were detected through the assay of HbA1c in diabetic patients. Using chromatography on Bio-Rex 70 resin, one large peak replaced the usually well resolved peaks of HbA1a + b and HbA1c. The species that overlapped the latter fractions was identified as HbJ1c. HbJ-Baltimore itself was identified using HPLC of the beta-chain tryptic peptides. This observation emphasizes the errors that hemoglobin variants may introduce in the assay of HbA1c.

  5. Cell adhesion to fibrillin-1: identification of an Arg-Gly-Asp-dependent synergy region and a heparin-binding site that regulates focal adhesion formation

    DEFF Research Database (Denmark)

    Bax, Daniel V; Mahalingam, Yashithra; Cain, Stuart

    2007-01-01

    We have defined the molecular basis of cell adhesion to fibrillin-1, the major structural component of extracellular microfibrils that are associated with elastic fibres. Using human dermal fibroblasts, and recombinant domain swap fragments containing the Arg-Gly-Asp motif, we have demonstrated...... a requirement for upstream domains for integrin-alpha(5)beta(1)-mediated cell adhesion and migration. An adjacent heparin-binding site, which supports focal adhesion formation, was mapped to the fibrillin-1 TB5 motif. Site-directed mutagenesis revealed two arginine residues that are crucial for heparin binding...

  6. Nástroje pro testování bezpečnosti aplikací v ASP.NET

    OpenAIRE

    Kikerle, Martin

    2015-01-01

    The purpose of this thesis is to create a methodology which helps ASP.NET developers to test their web applications for the presence of the most common vulnerabilities. The theoretical part is focused on the ways of application testing and briefly describes ten of the most common web application vulnerabilities, so-called OWASP Top Ten 2013. The web application testing methodology is included in the practical part. A process of the application testing is the key part of the methodology. The d...

  7. Depositional processes, triggering mechanisms and sediment composition of carbonate gravity flow deposits: examples from the Late Cretaceous of the south-central Pyrenees, Spain

    Science.gov (United States)

    Drzewiecki, Peter A.; Simó, J. Antonio

    2002-01-01

    Cenomanian through Coniacian strata near the town of Sopeira in the south-central Pyrenees (northern Spain) are composed of a variety of autochthonous and allochthonous carbonate slope lithologies that are divided into six depositional sequences based on facies distribution patterns and stratal relationships. The sequences record three major phases of platform margin evolution: rifting, burial, and exhumation. During the first phase (sequences UK-1, UK-2, UK-3, UK-4, and lower UK-5), deposition occurred on the edge of a wrench basin, and a normal fault located beneath the platform margin strongly influenced slope evolution. Background hemipelagic sediments on the slope were commonly redeposited by submarine slumps and slides. More intense reworking resulted in matrix-supported, slope-derived megaconglomerates (debrites). During the Cenomanian and Turonian, seismically triggered debris flows originated at the platform margin, bypassed the upper slope, and were deposited on the lower slope as polymictic, clast-supported, matrix-rich megabreccias. The megabreccias form channelized and sheet-like bodies with erosional basal surfaces. Shallow carbonate environments backstepped during the Late Turonian and Coniacian, but displacement along the fault at this time resulted in the development of a steep submarine scarp and the exposure of Cenomanian and Lower Turonian strata to submarine erosion. Matrix-poor, margin-derived megabreccias form a thick talus pile at the base of the scarp. Some of the breccias were transported into the basin as debris falls, forming sheet-like beds. Marl eventually buried the Coniacian scarp in sequence UK-5, resulting in the second major phase of platform slope evolution. The slope profile at this time was relatively gentle, and redeposited material is less common. In the third phase (sequence UK-6), tectonically induced bankward erosion during the Santonian resulted in a high (greater than 800 m) erosional scarp with a regional east

  8. Along-strike thickness variations of décollement levels controlling lateral changes in fold-and-thrust belts: the Barbastro-Balaguer Anticline (Southern Pyrenees)

    Science.gov (United States)

    Santolaria, Pablo; Calvín, Pablo; Pueyo, Emilio L.; Soto, Ruth; Ayala, Concepción; Casas, Antonio; Oliván, Carlota; Luzón, Aránzazu

    2017-04-01

    The subsurface vertical and lateral distribution of two evaporitic levels (Middle-Upper Triassic and Eocene) is here studied to better understand their role as a controlling factor on the geometry and kinematics of the central part of the South Pyrenean deformation front. Field work with hundreds of bedding attitudes, 5 exploration boreholes (up to 5000 m deep), the residual Bouguer anomaly and the interpretation of 27 seismic reflection profiles (approximately 440 km of sections) crossing the different allochthonous units and the adjacent Ebro foreland basin have been analysed in this work. Subsoil information is crucial because part of the studied area is extensively covered by the Oligocene-Miocene molasse that prevents any direct observation of the structure underneath to be done. Interpreted key horizons and faults have been identified from the lithological description of partially reinterpreted lithological well data and surface geology. Lithological well data has been anchored to seismic profiles by means of sonic log data. Kingdom software has been used to perform the 2D interpretation of seismic reflection profiles, supported by 2.5D gravity modelling, in a georeferenced workspace and then, surfaces of key horizons and faults were built and assembled together in a 3D model. The studied area is characterized from north to south by several minor thrust sheets, detached on the Middle-Upper Triassic décollement, which represents the basal décollement of the Pyrenees. Some of them can be linked with the South Pyrenean Frontal Thrust (SPFT) and others have structural continuation towards the NW. The SPFT displays a N160E trend and its hanging wall progressively deepens towards the west. To the east, this structure locally crops out. On the other hand, the footwall geometry of the SPBT shows very distinct features; the western part (External Sierras sector) displays a single ramp with moderate-high angle (20-30°) while the eastern part (linking zone to the

  9. Functional enhancement of neuronal cell behaviors and differentiation by elastin-mimetic recombinant protein presenting Arg-Gly-Asp peptides

    Directory of Open Access Journals (Sweden)

    Jeon Won

    2012-09-01

    Full Text Available Abstract Background Integrin-mediated interaction of neuronal cells with extracellular matrix (ECM is important for the control of cell adhesion, morphology, motility, and differentiation in both in vitro and in vivo systems. Arg-Gly-Asp (RGD sequence is one of the most potent integrin-binding ligand found in many native ECM proteins. An elastin-mimetic recombinant protein, TGPG[VGRGD(VGVPG6]20WPC, referred to as [RGD-V6]20, contains multiple RGD motifs to bind cell-surface integrins. This study aimed to investigate how surface-adsorbed recombinant protein can be used to modulate the behaviors and differentiation of neuronal cells in vitro. For this purpose, biomimetic ECM surfaces were prepared by isothermal adsorption of [RGD-V6]20 onto the tissue culture polystyrene (TCPS, and the effects of protein-coated surfaces on neuronal cell adhesion, spreading, migration, and differentiation were quantitatively measured using N2a neuroblastoma cells. Results The [RGD-V6]20 was expressed in E. coli and purified by thermally-induced phase transition. N2a cell attachment to either [RGD-V6]20 or fibronectin followed hyperbolic binding kinetics saturating around 2 μM protein concentration. The apparent maximum cell binding to [RGD-V6]20 was approximately 96% of fibronectin, with half-maximal adhesion on [RGD-V6]20 and fibronectin occurring at a coating concentration of 2.4 × 10-7 and 1.4 × 10-7 M, respectively. The percentage of spreading cells was in the following order of proteins: fibronectin (84.3% ± 6.9% > [RGD-V6]20 (42.9% ± 6.5% > [V7]20 (15.5% ± 3.2% > TCPS (less than 10%. The migration speed of N2a cells on [RGD-V6]20 was similar to that of cells on fibronectin. The expression of neuronal marker proteins Tuj1, MAP2, and GFAP was approximately 1.5-fold up-regulated by [RGD-V6]20 relative to TCPS. Moreover, by the presence of both [RGD-V6]20 and RA, the expression levels of NSE, TuJ1, NF68, MAP2, and GFAP

  10. Landslide Buries Valley of the Geysers

    Science.gov (United States)

    2007-01-01

    Geysers are a rare natural phenomena found only in a few places, such as New Zealand, Iceland, the United States (Yellowstone National Park), and on Russia's far eastern Kamchatka Peninsula. On June 3, 2007, one of these rare geyser fields was severely damaged when a landslide rolled through Russia's Valley of the Geysers. The landslide--a mix of mud, melting snow, trees, and boulders--tore a scar on the land and buried a number of geysers, thermal pools, and waterfalls in the valley. It also blocked the Geyser River, causing a new thermal lake to pool upstream. The Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) on NASA's Terra satellite captured this infrared-enhanced image on June 11, 2007, a week after the slide. The image shows the valley, the landslide, and the new thermal lake. Even in mid-June, just days from the start of summer, the landscape is generally covered in snow, though the geologically heated valley is relatively snow free. The tree-covered hills are red (the color of vegetation in this false-color treatment), providing a strong contrast to the aquamarine water and the gray-brown slide. According to the Russian News and Information Agency (RIA) [English language], the slide left a path roughly a kilometer and a half (one mile) long and 200 meters (600 feet) wide. Within hours of the landslide, the water in the new lake inundated a number of additional geysers. The geysers directly buried under the landslide now lie under as much as 60 meters (180 feet) of material, according to RIA reports. It is unlikely that the geysers will be able to force a new opening through this thick layer, adds RIA. Among those directly buried is Pervenets (Firstborn), the first geyser found in the valley, in 1941. Other geysers, such as the Bolshoi (Greater) and Maly (Lesser) Geysers, were silenced when buried by water building up behind the new natural dam. According to Vladimir and Andrei Leonov of the Russian Federation Institute of

  11. Valley-orbit hybrid states in Si quantum dots

    Science.gov (United States)

    Gamble, John; Friesen, Mark; Coppersmith, S. N.

    2013-03-01

    The conduction band for electrons in layered Si nanostructures oriented along (001) has two low-lying valleys. Most theoretical treatments assume that these valleys are decoupled from the long-wavelength physics of electron confinement. In this work, we show that even a minimal amount of disorder (a single atomic step at the quantum well interface) is sufficient to mix valley states and electron orbitals, causing a significant distortion of the long-wavelength electron envelope. For physically realistic electric fields and dot sizes, this valley-orbit coupling impacts all electronic states in Si quantum dots, implying that one must always consider valley-orbit hybrid states, rather than distinct valley and orbital degrees of freedom. We discuss the ramifications of our results on silicon quantum dot qubits. This work was supported in part by ARO (W911NF-08-1-0482) and NSF (DMR-0805045).

  12. Valley Hall effect and Nernst effect in strain engineered graphene

    Science.gov (United States)

    Niu, Zhi Ping; Yao, Jian-ming

    2018-04-01

    We theoretically predict the existence of tunneling valley Hall effect and Nernst effect in the normal/strain/normal graphene junctions, where a strained graphene is sandwiched by two normal graphene electrodes. By applying an electric bias a pure transverse valley Hall current with longitudinal charge current is generated. If the system is driven by a temperature bias, a valley Nernst effect is observed, where a pure transverse valley current without charge current propagates. Furthermore, the transverse valley current can be modulated by the Fermi energy and crystallographic orientation. When the magnetic field is further considered, we obtain a fully valley-polarized current. It is expected these features may be helpful in the design of the controllable valleytronic devices.

  13. Hydrologic reconnaissance of Rush Valley, Tooele County, Utah

    Science.gov (United States)

    Hood, James W.; Price, Don; Waddell, K.M.

    1969-01-01

    This report is the third in a series by the U. S. Geological Survey in cooperation with the Utah Department of Natural Resources, Division of Water Rights, which describes the water resources of the western basins of Utah. Its purpose is to present available hydrologic data for Rush Valley, to provide an evaluation of the potential water-resources development of the valley, and to identify needed studies that would help provide an understanding of the valley's water supply.

  14. Hydrologic reconnaissance of Skull Valley, Tooele County, Utah

    Science.gov (United States)

    Hood, James W.; Waddell, K.M.

    1968-01-01

    This report is the second in a series by the U.S. Geological Survey in cooperation with the Utah Department of Natural Resources, Division of Water Rights, which describes the water resources of the western basins of Utah. Its purpose is to present available hydrologic data on Skull Valley, to provide an evaluation of the potential water-resource development of the valley, and to identify needed studies that would help provide an understandingof the valley's water supply.

  15. OBSERVED AIR POLLUTION SPECIFICS OF THE VALLEY-BASED TOWNS

    OpenAIRE

    ZOLTÁN UTASI; ILONA PAJTÓK-TARI; JÁNOS MIKA; CSABA PATKÓS; ANTAL TÓTH

    2012-01-01

    Observed air pollution specifics of the valley-based towns. There are 21 valley-based towns in the 100 most populated ones of Hungary. This topographical feature may be advantageous due to mezoscale circulation between the valley or basin, containing these settlements and the surrounding hills. On the other hand, the hills form a mechanical obstacle that may limit the vertical mixing of pollution. Final result of these counteracting features are analysed by comparing air pollution characteris...

  16. Climatology of atmospheric PM10 concentration in the Po Valley

    OpenAIRE

    Alessandro, Bigi; Ghermandi, Grazia

    2014-01-01

    The limits to atmospheric pollutant concentration set by the European Commission provide a challenging target for the municipalities in the Po Valley, because of the characteristic climatic conditions and high population density of this region. In order to assess climatology and trends in the concentration of atmospheric particles in the Po Valley, a dataset of PM10 data from 41 sites across the Po Valley have been analysed, including both traffic and backgr...

  17. Heterozygous p.Asp50Asn mutation in the GJB2 gene in two Cameroonian patients with keratitis-ichthyosis-deafness (KID) syndrome.

    Science.gov (United States)

    Wonkam, Ambroise; Noubiap, Jean Jacques N; Bosch, Jason; Dandara, Collet; Toure, Geneviève Bengono

    2013-08-07

    Keratitis-Ichthyosis-Deafness (KID) syndrome (OMIM 148210) is a congenital ectodermal defect that consists of an atypical ichthyosiform erythroderma associated with congenital sensorineural deafness. KID appears to be genetically heterogeneous and most cases are caused by GJB2 mutations. Mutations in African patients have been rarely described. We report on two unrelated Cameroonian individuals affected with sporadic KID, presenting with the classic phenotypic triad. The two patients were heterozygous for the most frequent p.Asp50Asn mutation. This first report in patients from sub-Saharan African origin supports the hypothesis that the occurrence of KID due to p.Asp50Asn mutation in GJB2 seems not to be population specific. Our finding has implication in medical genetic practice, specifically in the molecular diagnosis of KID in Africans. These cases also reveal and emphasize the urgent need to develop appropriate policies to care for patients with rare/orphan diseases in Sub-Saharan Africa, as many of these cases become more and more recognizable.

  18. Development of a programme about Food and Nutrition Education in school children in the State School “La Serranica” in Aspe (Alicante: A pilot experience

    Directory of Open Access Journals (Sweden)

    Alba Martínez-García

    2016-05-01

    Full Text Available Introduction: Obesity and overweight are among the main risk factors in the world today. In Spain, more than half of the children are above their ideal weight. The aim of this study was to show the pilot experience of the activities of food and nutrition education which were carried out in the State School “La Serranica “ in Aspe (Spain to promote the acquisition of knowledge and healthy eating habits and assess the impact of such intervention. Material and Methods: The study was developed in a population of 28 students of 3rd of Primary School in “La Serranica” in Aspe (Alicante, Spain. Results: The student response was positive. After the intervention, more than 70% of children placed the food correctly in the pyramid, identified season fruits and vegetables, and a healthy breakfast. However, the percentage decreased two years later, except for recognizing a healthy breakfast (89%. In relation to lunch, consumption of fruits increased in 18% at the end of the period of the activities, and consumption of pastries and commercial juices decreased. Anybody took for lunch pastries and most of them consumed sandwich and/or fruit two years after intervention. Conclusions: An educational intervention about food and nutrition produces positive changes on the population, significantly improving knowledge and consumption of healthy food and decreasing consumption of unhealthy foods. Therefore, it could be said that nutrition programmes education are needed in today’s society to prevent the problem of overweight and obesity and promote healthy habits.

  19. Appearance of Planktothrix rubescens Bloom with [D-Asp3, Mdha7]MC–RR in Gravel Pit Pond of a Shallow Lake-Dominated Area

    Directory of Open Access Journals (Sweden)

    Gábor Vasas

    2013-12-01

    Full Text Available Blooms of toxic cyanobacteria are well-known phenomena in many regions of the world. Microcystin (MC, the most frequent cyanobacterial toxin, is produced by entirely different cyanobacteria, including unicellular, multicellular filamentous, heterocytic, and non-heterocytic bloom-forming species. Planktothrix is one of the most important MC-producing genera in temperate lakes. The reddish color of cyanobacterial blooms viewed in a gravel pit pond with the appearance of a dense 3 cm thick layer (biovolume: 28.4 mm3 L−1 was an unexpected observation in the shallow lake-dominated alluvial region of the Carpathian Basin. [d-Asp3, Mdha7]MC–RR was identified from the blooms sample by MALDI-TOF and NMR. Concentrations of [d-Asp3, Mdha7]MC–RR were measured by capillary electrophoresis to compare the microcystin content of the field samples and the isolated, laboratory-maintained P. rubescens strain. In analyzing the MC gene cluster of the isolated P. rubescens strain, a deletion in the spacer region between mcyE and mcyG and an insertion were located in the spacer region between mcyT and mcyD. The insertion elements were sequenced and partly identified. Although some invasive tropical cyanobacterial species have been given a great deal of attention in many recent studies, our results draw attention to the spread of the alpine organism P. rubescens as a MC-producing, bloom-forming species.

  20. Simultaneous determination of a novel oral Janus kinase inhibitor ASP015K and its sulfated metabolite in rat plasma using LC-MS/MS.

    Science.gov (United States)

    Oda, Kazuo; Mera, Katsumi; Nagasaka, Yasuhisa; Tokoro, Kazumi

    2015-07-01

    A sensitive and selective liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed for determining the concentrations of novel Janus kinase inhibitor ASP015K and its sulfated metabolite M2 in rat plasma. This method involves solid-phase extraction (SPE) from 25 μL of rat plasma. LC separation was performed on an Inertsil PH-3 column (100 mm L ×4.6 mm I.D., 5 µm) with a mobile phase consisting of 10 mM ammonium acetate and methanol under linear gradient conditions. Analytes were introduced to the LC-MS/MS through an electrospray ionization source and detected in positive-ion mode using selected reaction monitoring. Standard curves were linear from 0.25 to 500 ng/mL (r ≥0.9964). This assay enabled quantification of ASP015K and M2 at a concentration as low as 0.25 ng/mL in rat plasma. Validation data demonstrated that the method is selective, sensitive and accurate. Further, we also successfully applied this method to a preclinical pharmacokinetic study in rats. Copyright © 2014 John Wiley & Sons, Ltd.

  1. Electrostatics of the photosynthetic bacterial reaction center. Protonation of Glu L 212 and Asp L 213 - A new method of calculation.

    Science.gov (United States)

    Ptushenko, Vasily V; Cherepanov, Dmitry A; Krishtalik, Lev I

    2015-12-01

    Continuum electrostatic calculation of the transfer energies of anions from water into aprotic solvents gives the figures erroneous by order of magnitude. This is due to the hydrogen bond disruption that suggests the necessity to reconsider the traditional approach of the purely electrostatic calculation of the transfer energy from water into protein. In this paper, the method combining the experimental estimates of the transfer energies from water into aprotic solvent and the electrostatic calculation of the transfer energies from aprotic solvent into protein is proposed. Hydrogen bonds between aprotic solvent and solute are taken into account by introducing an imaginary aprotic medium incapable to form hydrogen bonds with the solute. Besides, a new treatment of the heterogeneous intraprotein dielectric permittivity based on the microscopic protein structure and electrometric measurements is elaborated. The method accounts semi-quantitatively for the electrostatic effect of diverse charged amino acid substitutions in the donor and acceptor parts of the photosynthetic bacterial reaction center from Rhodobacter sphaeroides. Analysis of the volatile secondary acceptor site QB revealed that in the conformation with a minimal distance between quinone QB and Glu L 212 the proton uptake upon the reduction of QB is prompted by Glu L 212 in alkaline and by Asp L 213 in slightly acidic regions. This agrees with the pH dependences of protonation degrees and the proton uptake. The method of pK calculation was applied successfully also for dissociation of Asp 26 in bacterial thioredoxin. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Competitive formation of b(2) and c(2)-H2O ions from b(3) ions containing Asp residue during tandem mass spectrometry: the influence of neighboring Arg.

    Science.gov (United States)

    Guo, Mengzhe; Guo, Cheng; Pan, Yuanjiang

    2014-08-01

    The fragmentation of b3 ions derived from protonated Arg-Xxx-Asp-Ala-Ala (Xxx = Ala, Asp, Glu, Cys) and Arg-Xxx-Glu-Ala-Ala was investigated by electrospray ionization tandem mass spectrometry (MS (n) ) with collision-induced dissociation. A particular ion, which is 1 Da less than b2 ion, is shown to be the c2-H2O ion. The mechanism for its formation involved the aspartic acid in the third position easily losing anhydride to form a c2 ion, which then lost water to form an eight-membered ring of azacyclooctane derivative under the participation of the guanidine of the N-terminal arginine. However, this phenomenon was not observed when the aspartic acid was replaced by glutamic acid. The Amber program was used to determine the conformation of the original c2 residue from the dynamic energy perspective, and then density functional theory-based calculations and changing N-terminal amino acid from arginine to phenylalanine supported this mechanism.

  3. Analysis of ozone in the San Joaquin Valley of California

    Science.gov (United States)

    Dabdub, Donald; DeHaan, Laurel L.; Seinfeld, John H.

    The dynamics of ozone in the San Joaquin Valley of central California are studied by systematic diagnostic runs of the three-dimensional SARMAP Air Quality Model. Air quality in the San Joaquin Valley is the result of a complex combination of local and transported emissions. Simulations show that relatively brisk winds at points of inflow to the Valley produce a strong dependence of ozone in the Valley on upwind conditions. Furthermore, NO x influx from boundaries and local emissions has significantly greater impact on ozone production than ROG influx and emissions.

  4. Disorder-dependent valley properties in monolayer WSe2

    KAUST Repository

    Tran, Kha

    2017-07-19

    We investigate the effect of disorder on exciton valley polarization and valley coherence in monolayer WSe2. By analyzing the polarization properties of photoluminescence, the valley coherence (VC) and valley polarization (VP) are quantified across the inhomogeneously broadened exciton resonance. We find that disorder plays a critical role in the exciton VC, while affecting VP less. For different monolayer samples with disorder characterized by their Stokes shift (SS), VC decreases in samples with higher SS while VP does not follow a simple trend. These two methods consistently demonstrate that VC as defined by the degree of linearly polarized photoluminescence is more sensitive to disorder, motivating further theoretical studies.

  5. A cyclic nucleotide-gated channel mutation associated with canine daylight blindness provides insight into a role for the S2 segment tri-Asp motif in channel biogenesis.

    Directory of Open Access Journals (Sweden)

    Naoto Tanaka

    Full Text Available Cone cyclic nucleotide-gated channels are tetramers formed by CNGA3 and CNGB3 subunits; CNGA3 subunits function as homotetrameric channels but CNGB3 exhibits channel function only when co-expressed with CNGA3. An aspartatic acid (Asp to asparagine (Asn missense mutation at position 262 in the canine CNGB3 (D262N subunit results in loss of cone function (daylight blindness, suggesting an important role for this aspartic acid residue in channel biogenesis and/or function. Asp 262 is located in a conserved region of the second transmembrane segment containing three Asp residues designated the Tri-Asp motif. This motif is conserved in all CNG channels. Here we examine mutations in canine CNGA3 homomeric channels using a combination of experimental and computational approaches. Mutations of these conserved Asp residues result in the absence of nucleotide-activated currents in heterologous expression. A fluorescent tag on CNGA3 shows mislocalization of mutant channels. Co-expressing CNGB3 Tri-Asp mutants with wild type CNGA3 results in some functional channels, however, their electrophysiological characterization matches the properties of homomeric CNGA3 channels. This failure to record heteromeric currents suggests that Asp/Asn mutations affect heteromeric subunit assembly. A homology model of S1-S6 of the CNGA3 channel was generated and relaxed in a membrane using molecular dynamics simulations. The model predicts that the Tri-Asp motif is involved in non-specific salt bridge pairings with positive residues of S3/S4. We propose that the D262N mutation in dogs with CNGB3-day blindness results in the loss of these inter-helical interactions altering the electrostatic equilibrium within in the S1-S4 bundle. Because residues analogous to Tri-Asp in the voltage-gated Shaker potassium channel family were implicated in monomer folding, we hypothesize that destabilizing these electrostatic interactions impairs the monomer folding state in D262N mutant CNG

  6. Surface slip during large Owens Valley earthquakes

    KAUST Repository

    Haddon, E. K.

    2016-01-10

    The 1872 Owens Valley earthquake is the third largest known historical earthquake in California. Relatively sparse field data and a complex rupture trace, however, inhibited attempts to fully resolve the slip distribution and reconcile the total moment release. We present a new, comprehensive record of surface slip based on lidar and field investigation, documenting 162 new measurements of laterally and vertically displaced landforms for 1872 and prehistoric Owens Valley earthquakes. Our lidar analysis uses a newly developed analytical tool to measure fault slip based on cross-correlation of sublinear topographic features and to produce a uniquely shaped probability density function (PDF) for each measurement. Stacking PDFs along strike to form cumulative offset probability distribution plots (COPDs) highlights common values corresponding to single and multiple-event displacements. Lateral offsets for 1872 vary systematically from approximate to 1.0 to 6.0 m and average 3.31.1 m (2 sigma). Vertical offsets are predominantly east-down between approximate to 0.1 and 2.4 m, with a mean of 0.80.5 m. The average lateral-to-vertical ratio compiled at specific sites is approximate to 6:1. Summing displacements across subparallel, overlapping rupture traces implies a maximum of 7-11 m and net average of 4.41.5 m, corresponding to a geologic M-w approximate to 7.5 for the 1872 event. We attribute progressively higher-offset lateral COPD peaks at 7.12.0 m, 12.8 +/- 1.5 m, and 16.6 +/- 1.4 m to three earlier large surface ruptures. Evaluating cumulative displacements in context with previously dated landforms in Owens Valley suggests relatively modest rates of fault slip, averaging between approximate to 0.6 and 1.6 mm/yr (1 sigma) over the late Quaternary.

  7. Environmental Assessment for the Saline Valley Radar Facility Project Saline Valley, California

    Science.gov (United States)

    2003-12-01

    positions. In 1999, a Marine Corps jet aircraft crashed in the Saline Valley area. Because of the lack of radar data, the search area initially...Sauromalus obesus), zebra-tailed lizards (Callisaurus draconoides), desert iguanas Section 3.0 – Description of Proposed Action FINAL December

  8. 78 FR 59840 - Revisions to the California State Implementation Plan, Antelope Valley Air Quality Management...

    Science.gov (United States)

    2013-09-30

    ...] Revisions to the California State Implementation Plan, Antelope Valley Air Quality Management District... of plan. * * * * * (c) * * * (428) * * * (i) * * * (B) Antelope Valley Air Quality Management...) * * * (i) * * * (B) Antelope Valley Air Quality Management District. (1) Rule 431.1, ``Sulfur Content of...

  9. 78 FR 45114 - Revisions to the California State Implementation Plan, Antelope Valley Air Quality Management...

    Science.gov (United States)

    2013-07-26

    ... the California State Implementation Plan, Antelope Valley Air Quality Management District AGENCY... the Antelope Valley Air Quality Management District (AVAQMD) portion of the California State... for the South Coast Air Quality Management District (SCAQMD). The Antelope Valley Air Pollution...

  10. [The Great Rift Valley. Parasitological results].

    Science.gov (United States)

    Nozais, J P

    1985-01-01

    East Africa is separated from the continent by the Great Rift Valley which was created at the end of the secondary era limiting then the East Africa under-continent with peculiar fauna and flora features. A several million years long isolation, during the tertiary era, seems to explain that a certain number of protozoan and helminthic diseases present peculiar clinical, epidemiological, therapeutical and parasitological features. The occurrence of those peculiar strains tends to indicate that in this region, for example, the resistance of P. falciparum to amino-4-quinolines is a regional feature which should not largely expand to the rest of the African continent.

  11. Neuroimaging Features of San Luis Valley Syndrome

    Directory of Open Access Journals (Sweden)

    Matthew T. Whitehead

    2015-01-01

    Full Text Available A 14-month-old Hispanic female with a history of double-outlet right ventricle and developmental delay in the setting of recombinant chromosome 8 syndrome was referred for neurologic imaging. Brain MR revealed multiple abnormalities primarily affecting midline structures, including commissural dysgenesis, vermian and brainstem hypoplasia/dysplasia, an interhypothalamic adhesion, and an epidermoid between the frontal lobes that enlarged over time. Spine MR demonstrated hypoplastic C1 and C2 posterior elements, scoliosis, and a borderline low conus medullaris position. Presented herein is the first illustration of neuroimaging findings from a patient with San Luis Valley syndrome.

  12. The uncanny valley in games and animation

    CERN Document Server

    Tinwell, Angela

    2014-01-01

    Advances in technology have enabled animators and video game designers to design increasingly realistic, human-like characters in animation and games. Although it was intended that this increased realism would allow viewers to appreciate the emotional state of characters, research has shown that audiences often have a negative reaction as the human likeness of a character increases. This phenomenon, known as the Uncanny Valley, has become a benchmark for measuring if a character is believably realistic and authentically human like. This book is an essential guide on how to overcome the Uncanny

  13. Extracellular pH Regulates Zinc Signaling via an Asp Residue of the Zinc-sensing Receptor (ZnR/GPR39)*

    Science.gov (United States)

    Cohen, Limor; Asraf, Hila; Sekler, Israel; Hershfinkel, Michal

    2012-01-01

    Zinc activates a specific Zn2+-sensing receptor, ZnR/GPR39, and thereby triggers cellular signaling leading to epithelial cell proliferation and survival. Epithelial cells that express ZnR, particularly colonocytes, face frequent changes in extracellular pH that are of physiological and pathological implication. Here we show that the ZnR/GPR39-dependent Ca2+ responses in HT29 colonocytes were maximal at pH 7.4 but were reduced by about 50% at pH 7.7 and by about 62% at pH 7.1 and were completely abolished at pH 6.5. Intracellular acidification did not attenuate ZnR/GPR39 activity, indicating that the pH sensor of this protein is located on an extracellular domain. ZnR/GPR39-dependent activation of extracellular-regulated kinase (ERK)1/2 or AKT pathways was abolished at acidic extracellular pH of 6.5. A similar inhibitory effect was monitored for the ZnR/GPR39-dependent up-regulation of Na+/H+ exchange activity at pH 6.5. Focusing on residues putatively facing the extracellular domain, we sought to identify the pH sensor of ZnR/GPR39. Replacing the histidine residues forming the Zn2+ binding site, His17 or His19, or other extracellular-facing histidines to alanine residues did not abolish the pH dependence of ZnR/GPR39. In contrast, replacing Asp313 with alanine resulted in similar Ca2+ responses triggered by ZnR/GPR39 at pH 7.4 or 6.5. This mutant also showed similar activation of ERK1/2 and AKT pathways, and ZnR-dependent up-regulation of Na+/H+ exchange at pH 7.4 and pH 6.5. Substitution of Asp313 to His or Glu residues restored pH sensitivity of the receptor. This indicates that Asp313, which was shown to modulate Zn2+ binding, is an essential residue of the pH sensor of GPR39. In conclusion, ZnR/GPR39 is tuned to sense physiologically relevant changes in extracellular pH that thus regulate ZnR-dependent signaling and ion transport activity. PMID:22879599

  14. Home destruction examination: Grass Valley Fire, Lake Arrowhead, California

    Science.gov (United States)

    Jack D. Cohen; Richard D. Stratton

    2008-01-01

    The Grass Valley Fire started October 22, 2007 at approximately 0508, one-mile west of Lake Arrowhead in the San Bernardino Mountains. Fuel and weather conditions were extreme due to drought, dry Santa Ana winds, and chaparral and conifer vegetation on steep terrain. The fire proceeded south through the Grass Valley drainage one-mile before impacting an area of dense...

  15. Prevalence of Rift Valley Fever among ruminants, Mayotte.

    Science.gov (United States)

    Cêtre-Sossah, Catherine; Pédarrieu, Aurélie; Guis, Hélène; Defernez, Cédric; Bouloy, Michèle; Favre, Jacques; Girard, Sébastien; Cardinale, Eric; Albina, Emmanuel

    2012-06-01

    Rift Valley fever threatens human and animal health. After a human case was confirmed in Comoros in 2007, 4 serosurveys among ruminants in Mayotte suggested that Rift Valley fever virus had been circulating at low levels since 2004, although no clinical cases occurred in animals. Entomologic and ecologic studies will help determine outbreak potential.

  16. Rift Valley fever potential mosquito vectors and their infection status ...

    African Journals Online (AJOL)

    Background: Rift Valley fever (RVF) is a mosquito-borne viral zoonotic disease. Rift Valley fever virus (RVFV) has been isolated from more than 40 species of mosquitoes from eight genera. This study was conducted to determine the abundance of potential mosquito vectors and their RVFV infection status in Ngorongoro ...

  17. Medicinal plants of Usherai valley, Dir, NWFP, Pakistan

    International Nuclear Information System (INIS)

    Hazarat, A.; Shah, J.; Ahmad, S.; Nasir, M.; Jan, A.K.; Skindar

    2010-01-01

    This research is based on the results of an ethno-botanical research conducted in Usherai Valley. The main objective was to enlist the wealth of medicinal plants. In total 50 species, belonging to 32 families of wild herbs, shrubs and trees were found to be used as medicinal plants by the inhabitants in the valley. (author)

  18. Some Environmental Issues of Inland Valleys: A Case Study | Asiam ...

    African Journals Online (AJOL)

    The study concluded that inland valleys can be real environmental liability because produce from such valleys can be polluted and hence can be a source of social conflict particularly when they fringe mineral concessions as the adverse impacts could be unfortunately attributed to mining activity and similar land uses.

  19. Influence of Valley Floor Landforms on Stream Ecosystems

    Science.gov (United States)

    Stanley V. Gregory; Gary A. Lamberti; Kelly M. S. Moore

    1989-01-01

    A hierarchical perspective of relationships between valley floor landforms, riparian plant communities, and aquatic ecosystems has been developed, based on studies of two fifth-order basins in the Cascade Mountains of Oregon. Retention of dissolved nitrogen and leaves were approximately 2-3 times greater in unconstrained reaches than in constrained reaches. Both valley...

  20. 27 CFR 9.208 - Snake River Valley.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Snake River Valley. 9.208... Snake River Valley. (a) Name. The name of the viticultural area described in this section is “Snake River Valley”. For purposes of part 4 of this chapter, “Snake River Valley” is a term of viticultural...

  1. Adapting to Climate Change in Peru's Mantaro Valley | IDRC ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    The Instituto Geofísico del Perú (Peruvian Geophysics Institute), in the Ministry of Environment, has assessed the vulnerability of rural and urban settlements in the valley. Through several multidisciplinary studies, the Institute identified the level of risk that extreme weather events pose to the valley's population and public ...

  2. Rift Valley Fever Outbreak in Livestock, Mozambique, 2014

    Science.gov (United States)

    Coetzee, Peter; Mubemba, Benjamin; Nhambirre, Ofélia; Neves, Luis; Coetzer, J.A.W.; Venter, Estelle H.

    2016-01-01

    In early 2014, abortions and death of ruminants were reported on farms in Maputo and Gaza Provinces, Mozambique. Serologic analysis and quantitative and conventional reverse transcription PCR confirmed the presence of Rift Valley fever virus. The viruses belonged to lineage C, which is prevalent among Rift Valley fever viruses in southern Africa. PMID:27869589

  3. 78 FR 9686 - Valley Electric Association, Inc.; Notice of Filing

    Science.gov (United States)

    2013-02-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Valley Electric Association, Inc.; Notice of Filing Take notice that on February 1, 2013, Valley Electric Association, Inc. filed a notice of material changes in certain of the...

  4. Groundwater recharge on east side soils of the Salinas Valley

    Science.gov (United States)

    After four years of drought, groundwater levels in the Salinas Valley are at historically low levels which threaten to adversely affect farming in the Salinas Valley. Given the prospect of a strong El Niño this coming winter, it seems prudent to plan to capture as much of the rainfall as possible to...

  5. Esophageal cancer in north rift valley of western Kenya | Wakhisi ...

    African Journals Online (AJOL)

    Esophageal cancer in north rift valley of western Kenya. ... Our finding also contrast with an earlier reported study that indicated that Rift Valley is a low prevalence area for this type of cancer. The mean age ... This may lead to identification of molecular biomarkers to be used in future for the early detection of this neoplasm.

  6. Salts in the dry valleys of Antartica

    Science.gov (United States)

    Gibson, E. K., Jr.; Presley, B. J.; Hatfield, J.

    1984-01-01

    The Dry Valleys of Antarctica are examples of polar deserts which are rare geological features on the Earth. Such deserts typically have high salinities associated with their closed-basin waters and on many surficial materials throughout them. In order to examine the possible sources for the salts observed in association with the soils in the Dry Valleys. The chloride and bromide concentrations of the water leachates from 58 soils and core samples were measured. The Cl/Br ratio for seawater is 289 and ratios measured for most of the 58 soils studied (greater than 85% of the soils studied) was larger than the seawater ratio (ratios typically were greater than 1000 and ranged up to 50,000). The enrichment in Cl relative to Br is strong evidence that the alts present within the soils were derived from seawater during ordinary evaporation processes, and not from the deposition of Cl and Br from aerosols or from rock weathering as has often been suggested.

  7. Investigating the uncanny valley for prosthetic hands.

    Science.gov (United States)

    Poliakoff, Ellen; O'Kane, Sophie; Carefoot, Olivia; Kyberd, Peter; Gowen, Emma

    2018-02-01

    In 1970, Mori hypothesised the existence of an 'uncanny valley', whereby stimuli falling short of being fully human are found to be creepy or eerie. To investigate how eerie people find different prosthetic hands and whether perceptions of eeriness can be accounted for by categorical ambiguity. Students participated in computerised experiments during which photographic images of hands were presented. We compared photographs of prosthetic hands pre-selected as more (H+) or less human-like (H-), as well as mechanical and real hands. Participants rated the hands for eeriness and human-likeness, as well as performing a speeded classification (human/non-human) and location judgment (control) task. The H- prosthetic hands were rated as more eerie than the H+ prosthetic, mechanical and real hands, and this was unaffected by hand orientation. Participants were significantly slower to categorise the H+ prosthetic hands compared to the H- prosthetic and real hands, which was not due to generally slower responses to the H+ prosthetic hands (control task). People find prosthetic hands to be eerie, most consistently for less human-like prosthetic hands. This effect is not driven by ambiguity about whether to categorise the prosthetic hand as human or artificial. Clinical relevance More obviously artificial, less-realistic, prosthetic hands consistently generate a sense of eeriness, while more realistic prosthetic hands avoid the uncanny valley, at least on initial viewing. Thus, greater realism in prosthetic design may not always incur a cost, although the role of movement and cutaneous input requires further investigation.

  8. West Valley waste removal system study

    International Nuclear Information System (INIS)

    Janicek, G.P.

    1981-04-01

    This study addresses the specific task of removing high-level wastes from underground tanks at Western New York Nuclear Center and delivering them to an onsite waste solidification plant. It begins with a review of the design and construction features of the waste storage tanks pertinent to the waste removal task with particular emphasis on the unique and complex tank internals which severely complicate the task of removal. It follows with a review of tank cleaning techniques used and under study at both Hanford and Savannah River and previous studies proposing the use of these techniques at West Valley. It concludes from these reviews that existing techniques are not directly transferable to West Valley and that a new approach is required utilizing selected feature and attributes from existing methodology. The study also concludes, from an investigation of the constraints imposed by the processing facility, that waste removal will be intermittent, requiring batch transfer over the anticipated 3 years of processing operations. Based on these reviews and conclusions, the study proposes that the acid waste be processed first and that one of the 15,000-gallon acid tanks then be used for batch feeding the neutralized waste. The proposed system would employ commercially available pumping equipment to transfer the wastes from the batch tank to processing via existing process piping. A commercially available mixed-flow pump and eight turbine pumps would homogenize the neutralized waste in conjunction with eight custom-fabricated sluicers for periodic transfer to the batch tank

  9. Ius Chasma Tributary Valleys and Adjacent Plains

    Science.gov (United States)

    2006-01-01

    This image covers valley tributaries of Ius Chasma, as well as the plains adjacent to the valleys. Ius Chasma is one of several canyons that make up the Valles Marineris canyon system. Valles Marineris likely formed by extension associated with the growth of the large volcanoes and topographic high of Tharsis to the northwest. As the ground was pulled apart, large and deep gaps resulted in the valleys seen in the top and bottom of this HiRISE image. Ice that was once in the ground could have also melted to create additional removal of material in the formation of the valleys. HiRISE is able to see the rocks along the walls of both these valleys and also impact craters in the image. Rock layers that appear lower down in elevation appear rougher and are shedding boulders. Near the top of the walls and also seen in patches along the smooth plains are brighter layers. These brighter layers are not shedding boulders so they must represent a different kind of rock formed in a different kind of environment than those further down the walls. Because they are highest in elevation, the bright layers are youngest in age. HiRISE is able to see dozens of the bright layers, which are perhaps only a meter in thickness. Darker sand dunes and ripples cover most of the plains and fill the floors of impact craters. Image PSP_001351_1715 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on November 9, 2006. The complete image is centered at -8.3 degrees latitude, 275.4 degrees East longitude. The range to the target site was 254.3 km (158.9 miles). At this distance the image scale ranges from 25.4 cm/pixel (with 1 x 1 binning) to 101.8 cm/pixel (with 4 x 4 binning). The image shown here has been map-projected to 25 cm/pixel and north is up. The image was taken at a local Mars time of 3:32 PM and the scene is illuminated from the west with a solar incidence angle of 59 degrees, thus the sun was about 31

  10. Four newly recorded species of Dryopteridaceae from Kashmir valley, India

    Directory of Open Access Journals (Sweden)

    SHAKOOR AHMAD MIR

    2014-04-01

    Full Text Available Mir SA, Mishra AK, Reshi ZA, Sharma MP. 2014. Four newly recorded species of Dryopteridaceae from Kashmir valley, India. Biodiversitas 15: 6-11. Habitat diversity, elevation, cloud cover, rainfall, seasonal and temperature variations have created many ideal sites for the luxuriant growth of pteridophytes in the Kashmir valley, yet all the regions of the valley have not been surveyed. In Kashmir valley the family Dryopteridaceae is represented by 31 species. During the recent extensive field surveys of Shopian district four more species viz., Dryopteris caroli-hopei Fraser-Jenkins, Dryopteris blanfordii subsp. nigrosquamosa (Ching Fraser-Jenkins, Dryopteris pulvinulifera (Bedd. Kuntze and Polystichum Nepalense (Spreng C. Chr. have been recorded for the first time from the valley. The taxonomic description, synonyms, distribution and photographs of each species are given in this article.

  11. Role of Flexibility in Protein-DNA-Drug Recognition: The Case of Asp677Gly-Val703Ile Topoisomerase Mutant Hypersensitive to Camptothecin

    Science.gov (United States)

    D'Annessa, Ilda; Tesauro, Cinzia; Fiorani, Paola; Chillemi, Giovanni; Castelli, Silvia; Vassallo, Oscar; Capranico, Giovanni; Desideri, Alessandro

    2012-01-01

    Topoisomerases I are ubiquitous enzymes that control DNA topology within the cell. They are the unique target of the antitumor drug camptothecin that selectively recognizes the DNA-topoisomerase covalent complex and reversibly stabilizes it. The biochemical and structural-dynamical properties of the Asp677Gly-Val703Ile double mutant with enhanced CPT sensitivity have been investigated. The mutant displays a lower religation rate of the DNA substrate when compared to the wild-type protein. Analyses of the structural dynamical properties by molecular dynamics simulation show that the mutant has reduced flexibility and an active site partially destructured at the level of the Lys532 residue. These results demonstrate long-range communication mechanism where reduction of the linker flexibility alters the active site geometry with the consequent lowering of the religation rate and increase in drug sensitivity. PMID:22315664

  12. Role of Flexibility in Protein-DNA-Drug Recognition: The Case of Asp677Gly-Val703Ile Topoisomerase Mutant Hypersensitive to Camptothecin

    Directory of Open Access Journals (Sweden)

    Ilda D'Annessa

    2012-01-01

    Full Text Available Topoisomerases I are ubiquitous enzymes that control DNA topology within the cell. They are the unique target of the antitumor drug camptothecin that selectively recognizes the DNA-topoisomerase covalent complex and reversibly stabilizes it. The biochemical and structural-dynamical properties of the Asp677Gly-Val703Ile double mutant with enhanced CPT sensitivity have been investigated. The mutant displays a lower religation rate of the DNA substrate when compared to the wild-type protein. Analyses of the structural dynamical properties by molecular dynamics simulation show that the mutant has reduced flexibility and an active site partially destructured at the level of the Lys532 residue. These results demonstrate long-range communication mechanism where reduction of the linker flexibility alters the active site geometry with the consequent lowering of the religation rate and increase in drug sensitivity.

  13. Covalent attachment of cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) to poly(etheretherketone) surface by tailored silanization layers technique

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Yanyan [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Xiong, Chengdong [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Li, Xiaoyu [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China); Zhang, Lifang, E-mail: zhanglfcioc@163.com [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China)

    2014-11-30

    Highlights: • The carbonyl groups on PEEK surface were effectively reduced to hydroxyl groups using sodium borohydride. • Silanization layers technique was employed to immobilize the cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) on hydroxylation-pretreated PEEK sheet surface by covalent chemical attachment. • XPS, surface profiler and water contact angle measurements proved the presence of GRGD on PEEK surface. • Osteoblast-like cells (MC3T3-E1) attachment and proliferation were improved effectively on GRGD-modified PEEK surface. - Abstract: Poly(etheretherketone) (PEEK) is a rigid semicrystalline polymer that combines excellent mechanical properties, broad chemical resistance and bone-like stiffness and is widely used in biomedical fields. However, PEEK is naturally bioinert, leading to limited biomedical applications, especially when a direct bone-implant osteointegration is desired. In this study, a three-step reaction procedure was employed to immobilize the cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) on the surface of PEEK sheet by covalent chemical attachment to favor cell adhesion and proliferation. First, hydroxylation-pretreated PEEK surfaces were silanized with 7-Oct-1-enyltrichlorosilane (OETS) in dry cyclohexane, resulting in a silanization layer with terminal ethenyl. Second, the terminal ethylenic double bonds of the silanization layer on PEEK surface were converted to carboxyl groups through acidic potassium manganate oxidation. Finally, GRGD was covalently attached by carbodiimide mediated condensation between the carboxyl on PEEK surface and amine presents in GRGD. X-ray photoelectron spectroscopy (XPS), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, surface profiler and water contact angle measurements were applied to characterize the modified surfaces. The effect of cells attachment and proliferation on each specimen was investigated. Pre-osteoblast cells (MC3T3-E1) attachment, spreading and proliferation

  14. Molecular Characterization of Lys49 and Asp49 Phospholipases A2 from Snake Venom and Their Antiviral Activities against Dengue virus

    Science.gov (United States)

    Cecilio, Alzira B.; Caldas, Sergio; De Oliveira, Raiana A.; Santos, Arthur S. B.; Richardson, Michael; Naumann, Gustavo B.; Schneider, Francisco S.; Alvarenga, Valeria G.; Estevão-Costa, Maria I.; Fuly, Andre L.; Eble, Johannes A.; Sanchez, Eladio F.

    2013-01-01

    We report the detailed molecular characterization of two PLA2s, Lys49 and Asp49 isolated from Bothrops leucurus venom, and examined their effects against Dengue virus (DENV). The Bl-PLA2s, named BlK-PLA2 and BlD-PLA2, are composed of 121 and 122 amino acids determined by automated sequencing of the native proteins and peptides produced by digestion with trypsin. They contain fourteen cysteines with pIs of 9.05 and 8.18 for BlK- and BlD-PLA2s, and show a high degree of sequence similarity to homologous snake venom PLA2s, but may display different biological effects. Molecular masses of 13,689.220 (Lys49) and 13,978.386 (Asp49) were determined by mass spectrometry. DENV causes a prevalent arboviral disease in humans, and no clinically approved antiviral therapy is currently available to treat DENV infections. The maximum non-toxic concentration of the proteins to LLC-MK2 cells determined by MTT assay was 40 µg/mL for Bl-PLA2s (pool) and 20 µg/mL for each isoform. Antiviral effects of Bl-PLA2s were assessed by quantitative Real-Time PCR. Bl-PLA2s were able to reduce DENV-1, DENV-2, and DENV-3 serotypes in LLC-MK2 cells infection. Our data provide further insight into the structural properties and their antiviral activity against DENV, opening up possibilities for biotechnological applications of these Bl-PLA2s as tools of research. PMID:24131891

  15. Covalent attachment of cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) to poly(etheretherketone) surface by tailored silanization layers technique

    International Nuclear Information System (INIS)

    Zheng, Yanyan; Xiong, Chengdong; Li, Xiaoyu; Zhang, Lifang

    2014-01-01

    Highlights: • The carbonyl groups on PEEK surface were effectively reduced to hydroxyl groups using sodium borohydride. • Silanization layers technique was employed to immobilize the cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) on hydroxylation-pretreated PEEK sheet surface by covalent chemical attachment. • XPS, surface profiler and water contact angle measurements proved the presence of GRGD on PEEK surface. • Osteoblast-like cells (MC3T3-E1) attachment and proliferation were improved effectively on GRGD-modified PEEK surface. - Abstract: Poly(etheretherketone) (PEEK) is a rigid semicrystalline polymer that combines excellent mechanical properties, broad chemical resistance and bone-like stiffness and is widely used in biomedical fields. However, PEEK is naturally bioinert, leading to limited biomedical applications, especially when a direct bone-implant osteointegration is desired. In this study, a three-step reaction procedure was employed to immobilize the cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) on the surface of PEEK sheet by covalent chemical attachment to favor cell adhesion and proliferation. First, hydroxylation-pretreated PEEK surfaces were silanized with 7-Oct-1-enyltrichlorosilane (OETS) in dry cyclohexane, resulting in a silanization layer with terminal ethenyl. Second, the terminal ethylenic double bonds of the silanization layer on PEEK surface were converted to carboxyl groups through acidic potassium manganate oxidation. Finally, GRGD was covalently attached by carbodiimide mediated condensation between the carboxyl on PEEK surface and amine presents in GRGD. X-ray photoelectron spectroscopy (XPS), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, surface profiler and water contact angle measurements were applied to characterize the modified surfaces. The effect of cells attachment and proliferation on each specimen was investigated. Pre-osteoblast cells (MC3T3-E1) attachment, spreading and proliferation

  16. Molecular Characterization of Lys49 and Asp49 Phospholipases A2 from Snake Venom and Their Antiviral Activities against Dengue virus

    Directory of Open Access Journals (Sweden)

    Andre L. Fuly

    2013-10-01

    Full Text Available We report the detailed molecular characterization of two PLA2s, Lys49 and Asp49 isolated from Bothrops leucurus venom, and examined their effects against Dengue virus (DENV. The Bl-PLA2s, named BlK-PLA2 and BlD-PLA2, are composed of 121 and 122 amino acids determined by automated sequencing of the native proteins and peptides produced by digestion with trypsin. They contain fourteen cysteines with pIs of 9.05 and 8.18 for BlK- and BlD-PLA2s, and show a high degree of sequence similarity to homologous snake venom PLA2s, but may display different biological effects. Molecular masses of 13,689.220 (Lys49 and 13,978.386 (Asp49 were determined by mass spectrometry. DENV causes a prevalent arboviral disease in humans, and no clinically approved antiviral therapy is currently available to treat DENV infections. The maximum non-toxic concentration of the proteins to LLC-MK2 cells determined by MTT assay was 40 µg/mL for Bl-PLA2s (pool and 20 µg/mL for each isoform. Antiviral effects of Bl-PLA2s were assessed by quantitative Real-Time PCR. Bl-PLA2s were able to reduce DENV-1, DENV-2, and DENV-3 serotypes in LLC-MK2 cells infection. Our data provide further insight into the structural properties and their antiviral activity against DENV, opening up possibilities for biotechnological applications of these Bl-PLA2s as tools of research.

  17. New Constraints for Tectono-Thermal Alpine Evolution of the Pyrenees: Combining Zircon Fission-Track and (U-Th)/He Analyses with Raman Spectrometry and In-Situ K-Ar Geochronology

    Science.gov (United States)

    Waldner, M.; Bellahsen, N.; Mouthereau, F.; Pik, R.; Bernet, M.; Scaillet, S.; Rosenberg, C.

    2017-12-01

    The pyrenean range was formed by the convergence of European and Iberian plates following the inversion of the Mesozoic rifting in the north of Pyrenees. In the Axial Zone, the collision caused an antiformal nappe-stacking of tectonic units. Recent studies pointed out the importance of pre-collision structural and thermal inheritance that may play a major role for orogeny such as: 1) Paleozoic Variscan inheritance; 2) Mesozoic rift-related high geothermal gradients, which are maintained during the onset of convergence in the North Pyrenean Zone. From a mineralogical point of view, pre-collision feldspars have been destabilized and influenced the development of alpine phyllonite in brittle-ductile conditions which suggests a weak crustal behavior during the formation of the orogenic wedge. Our aim is to get a better understanding of alpine deformation and exhumation by coupling different thermochronological, geochronological and thermometric methods. We document the thermal evolution of each tectonic unit by using low-temperature thermochronometers (Zircon Fission Tracks, U-Th/He on zircons including laser ablation profiles). Our data on vertical profiles combined to existing dataset on apatite allows to model alpine exhumation across the Axial zone. Structural observations through alpine thrusts coupled to geochronology (in situ K/Ar on phengites), Raman and chlorite-phengite thermo(baro)metry provide new key data to unravel the alpine evolution of the Pyrenees. According to preliminary ZFT results on granite massifs in the central part of Pyrenean Axial zone (near ECORS profile), exhumation ages potentially indicates a migration of exhumation towards the south. Exhumation ages of the northern massifs seems to have preserved the North Pyrenean Cretaceous rift evolution. Further south, the onset of exhumation is as old as Paleocene, which precedes the Eocene ages of the literature. The low burial estimated in the northern massifs may indicate a high thermal gradient

  18. Water resources of Parowan Valley, Iron County, Utah

    Science.gov (United States)

    Marston, Thomas M.

    2017-08-29

    Parowan Valley, in Iron County, Utah, covers about 160 square miles west of the Red Cliffs and includes the towns of Parowan, Paragonah, and Summit. The valley is a structural depression formed by northwest-trending faults and is, essentially, a closed surface-water basin although a small part of the valley at the southwestern end drains into the adjacent Cedar Valley. Groundwater occurs in and has been developed mainly from the unconsolidated basin-fill aquifer. Long-term downward trends in groundwater levels have been documented by the U.S. Geological Survey (USGS) since the mid-1950s. The water resources of Parowan Valley were assessed during 2012 to 2014 with an emphasis on refining the understanding of the groundwater and surface-water systems and updating the groundwater budget.Surface-water discharge of five perennial mountain streams that enter Parowan Valley was measured from 2013 to 2014. The total annual surface-water discharge of the five streams during 2013 to 2014 was about 18,000 acre-feet (acre-ft) compared to the average annual streamflow of about 22,000 acre-ft from USGS streamgages operated on the three largest of these streams from the 1940s to the 1980s. The largest stream, Parowan Creek, contributes more than 50 percent of the annual surface-water discharge to the valley, with smaller amounts contributed by Red, Summit, Little, and Cottonwood Creeks.Average annual recharge to the Parowan Valley groundwater system was estimated to be about 25,000 acre-ft from 1994 to 2013. Nearly all recharge occurs as direct infiltration of snowmelt and rainfall on the Markagunt Plateau east of the valley. Smaller amounts of recharge occur as infiltration of streamflow and unconsumed irrigation water near the east side of the valley on alluvial fans associated with mountain streams at the foot of the Red Cliffs. Subsurface flow from the mountain block to the east of the valley is a significant source of groundwater recharge to the basin-fill aquifer

  19. Surface slip during large Owens Valley earthquakes

    Science.gov (United States)

    Haddon, E.K.; Amos, C.B.; Zielke, O.; Jayko, Angela S.; Burgmann, R.

    2016-01-01

    The 1872 Owens Valley earthquake is the third largest known historical earthquake in California. Relatively sparse field data and a complex rupture trace, however, inhibited attempts to fully resolve the slip distribution and reconcile the total moment release. We present a new, comprehensive record of surface slip based on lidar and field investigation, documenting 162 new measurements of laterally and vertically displaced landforms for 1872 and prehistoric Owens Valley earthquakes. Our lidar analysis uses a newly developed analytical tool to measure fault slip based on cross-correlation of sublinear topographic features and to produce a uniquely shaped probability density function (PDF) for each measurement. Stacking PDFs along strike to form cumulative offset probability distribution plots (COPDs) highlights common values corresponding to single and multiple-event displacements. Lateral offsets for 1872 vary systematically from ∼1.0 to 6.0 m and average 3.3 ± 1.1 m (2σ). Vertical offsets are predominantly east-down between ∼0.1 and 2.4 m, with a mean of 0.8 ± 0.5 m. The average lateral-to-vertical ratio compiled at specific sites is ∼6:1. Summing displacements across subparallel, overlapping rupture traces implies a maximum of 7–11 m and net average of 4.4 ± 1.5 m, corresponding to a geologic Mw ∼7.5 for the 1872 event. We attribute progressively higher-offset lateral COPD peaks at 7.1 ± 2.0 m, 12.8 ± 1.5 m, and 16.6 ± 1.4 m to three earlier large surface ruptures. Evaluating cumulative displacements in context with previously dated landforms in Owens Valley suggests relatively modest rates of fault slip, averaging between ∼0.6 and 1.6 mm/yr (1σ) over the late Quaternary.

  20. The carbon stable isotope biogeochemistry of streams, Taylor Valley, Antarctica

    International Nuclear Information System (INIS)

    Lyons, W.B.; Leslie, D.L.; Harmon, R.S.; Neumann, K.; Welch, K.A.; Bisson, K.M.; McKnight, D.M.

    2013-01-01

    Highlights: ► δ 13 C-DIC reported from McMurdo Dry Valleys, Antarctica, streams. ► Stream water δ 13 C PDB values range −9.4‰ to +5.1‰, largely inorganic in character. ► Atmospheric exchange is the dominant control on δ 13 C-DIC. - Abstract: The McMurdo Dry Valleys region of Antarctica is the largest ice-free region on the continent. This study reports the first C stable isotope measurements for dissolved inorganic C present in ephemeral streams in four dry valleys that flow for four to twelve weeks during the austral summer. One of these valleys, Taylor Valley, has been the focus of the McMurdo Dry Valleys Long-Term Ecological Research (MCM-LTER) program since 1993. Within Taylor Valley, numerous ephemeral streams deliver water to three perennially ice-covered, closed-basin lakes: Lake Fryxell, Lake Hoare, and Lake Bonney. The Onyx River in the Wright Valley, the longest river in Antarctica, flows for 40 km from the Wright Lower Glacier and Lake Brownworth at the foot of the glacier to Lake Vanda. Streamflow in the McMurdo Dry Valley streams is produced primarily from glacial melt, as there is no overland flow. However, hyporheic zone exchange can be a major hydrogeochemical process in these streams. Depending on landscape position, these streams vary in gradient, channel substrate, biomass abundance, and hyporheic zone extent. This study sampled streams from Taylor, Wright, Garwood, and Miers Valleys and conducted diurnal sampling of two streams of different character in Taylor Valley. In addition, transect sampling was undertaken of the Onyx River in Wright Valley. The δ 13 C PDB values from these streams span a range of greater than 14‰, from −9.4‰ to +5.1‰, with the majority of samples falling between −3‰ and +2‰, suggesting that the C stable isotope composition of dissolved C in McMurdo Dry Valley streams is largely inorganic in character. Because there are no vascular plants on this landscape and no groundwater input to these

  1. Hydrogeological reconnaissance study: Dyfi Valley, Wales

    International Nuclear Information System (INIS)

    Glendining, S.J.

    1981-10-01

    This report describes work carried out for the Department of the Environment as part of its research programme into radioactive waste management. It presents an account of a hydrogeological reconnaissance study in the Dyfi Valley area of Central Wales. Initially the purposes of such a study are given and the assumptions used in deriving parameters such as flow volume, path length and transit time in areas of massive fractured rocks are described. Using these assumptions with geological, topographic and hydrometeorological data the potential ranges in properties such as bulk hydraulic conductivity, path lengths, hydraulic gradients and volumes of groundwater flow have been determined. These ranges have been used to estimate solute transport model parameters. The limitations and usefulness of the reconnaissance study in planning research and siting exploratory boreholes in the Dyfi area are discussed. (author)

  2. Diagnostic approaches for Rift Valley fever.

    Science.gov (United States)

    Wilson, W C; Weingartl, H M; Drolet, B S; Davé, K; Harpster, M H; Johnson, P A; Faburay, B; Ruder, M G; Richt, J A; McVey, D S

    2013-01-01

    Disease outbreaks caused by arthropod-borne animal viruses (arboviruses) resulting in significant livestock and economic losses world-wide appear to be increasing. Rift Valley fever (RVF) virus is an important arbovirus that causes lethal disease in cattle, camels, sheep and goats in Sub-Saharan Africa. There is concern that this virus could spread because of global warming, increased animal trade or through bioterrorism. This paper discusses the current and developing approaches to diagnosis of RVF. Diagnostic assays are available for RVF, but availability can be limited and there is a need for global harmonization. Continued improvement of standard serological and viral genome amplification approaches, including new embedded/syndromic testing, biosensor, emerging virus detection and characterization technologies is needed.

  3. Rift Valley Fever, Sudan, 2007 and 2010

    Science.gov (United States)

    Aradaib, Imadeldin E.; Erickson, Bobbie R.; Elageb, Rehab M.; Khristova, Marina L.; Carroll, Serena A.; Elkhidir, Isam M.; Karsany, Mubarak E.; Karrar, AbdelRahim E.; Elbashir, Mustafa I.

    2013-01-01

    To elucidate whether Rift Valley fever virus (RVFV) diversity in Sudan resulted from multiple introductions or from acquired changes over time from 1 introduction event, we generated complete genome sequences from RVFV strains detected during the 2007 and 2010 outbreaks. Phylogenetic analyses of small, medium, and large RNA segment sequences indicated several genetic RVFV variants were circulating in Sudan, which all grouped into Kenya-1 or Kenya-2 sublineages from the 2006–2008 eastern Africa epizootic. Bayesian analysis of sequence differences estimated that diversity among the 2007 and 2010 Sudan RVFV variants shared a most recent common ancestor circa 1996. The data suggest multiple introductions of RVFV into Sudan as part of sweeping epizootics from eastern Africa. The sequences indicate recent movement of RVFV and support the need for surveillance to recognize when and where RVFV circulates between epidemics, which can make data from prediction tools easier to interpret and preventive measures easier to direct toward high-risk areas. PMID:23347790

  4. Functional ecology of an Antarctic Dry Valley

    Science.gov (United States)

    Chan, Yuki; Van Nostrand, Joy D.; Zhou, Jizhong; Pointing, Stephen B.

    2013-01-01

    The McMurdo Dry Valleys are the largest ice-free region in Antarctica and are critically at risk from climate change. The terrestrial landscape is dominated by oligotrophic mineral soils and extensive exposed rocky surfaces where biota are largely restricted to microbial communities, although their ability to perform the majority of geobiological processes has remained largely uncharacterized. Here, we identified functional traits that drive microbial survival and community assembly, using a metagenomic approach with GeoChip-based functional gene arrays to establish metabolic capabilities in communities inhabiting soil and rock surface niches in McKelvey Valley. Major pathways in primary metabolism were identified, indicating significant plasticity in autotrophic, heterotrophic, and diazotrophic strategies supporting microbial communities. This represents a major advance beyond biodiversity surveys in that we have now identified how putative functional ecology drives microbial community assembly. Significant differences were apparent between open soil, hypolithic, chasmoendolithic, and cryptoendolithic communities. A suite of previously unappreciated Antarctic microbial stress response pathways, thermal, osmotic, and nutrient limitation responses were identified and related to environmental stressors, offering tangible clues to the mechanisms behind the enduring success of microorganisms in this seemingly inhospitable terrain. Rocky substrates exposed to larger fluctuations in environmental stress supported greater functional diversity in stress-response pathways than soils. Soils comprised a unique reservoir of genes involved in transformation of organic hydrocarbons and lignin-like degradative pathways. This has major implications for the evolutionary origin of the organisms, turnover of recalcitrant substrates in Antarctic soils, and predicting future responses to anthropogenic pollution. PMID:23671121

  5. Direct measurement of exciton valley coherence in monolayer WSe2

    KAUST Repository

    Hao, Kai

    2016-02-29

    In crystals, energy band extrema in momentum space can be identified by a valley index. The internal quantum degree of freedom associated with valley pseudospin indices can act as a useful information carrier, analogous to electronic charge or spin. Interest in valleytronics has been revived in recent years following the discovery of atomically thin materials such as graphene and transition metal dichalcogenides. However, the valley coherence time—a crucial quantity for valley pseudospin manipulation—is difficult to directly probe. In this work, we use two-dimensional coherent spectroscopy to resonantly generate and detect valley coherence of excitons (Coulomb-bound electron–hole pairs) in monolayer WSe2 (refs ,). The imposed valley coherence persists for approximately one hundred femtoseconds. We propose that the electron–hole exchange interaction provides an important decoherence mechanism in addition to exciton population recombination. This work provides critical insight into the requirements and strategies for optical manipulation of the valley pseudospin for future valleytronics applications.

  6. Origin and evolution of valleys on Martian volcanoes

    Energy Technology Data Exchange (ETDEWEB)

    Gulick, V.C.; Baker, V.R. (Univ. of Arizona, Tucson (USA))

    1990-08-30

    Morphological analyses of six Martian volcanoes, Ceraunius Tholus, Hecates Tholus, Alba Patera, Hadriaca Patera, Apollinaris Patera, and Tyrrhena Patera, indicate that fluvial processes were the dominant influence in the initiation and subsequent development of many dissecting valleys. Lava processes and possibly volcanic density flows were also important as valley-forming processes. Fluvial valleys are especially well developed on Alba Patera, Ceraunius Tholus, and Hecates Tholus. These valleys are inset into the surrounding landscape. They formed in regions of subdued lava flow morphology, contain tributaries, and tend to widen slightly in the downstream direction. Lava channels on Alba Patera are located on the crest of lava flows and have a discontinuous, irregular surface morphology, and distributary patterns. These channels sometimes narrow toward their termini. Possible volcanic density flow channels are located on the northern flank of Ceraunius Tholus. Valleys dissecting Apollinaris Patera, Hadriaca Patera, and Tyrrhena Patera appear to have a complex evolution, probably a mixed fluvial and lava origin. They are inset into a subdued (possibly mantled) surface, lack tributaries, and either have fairly constant widths or widen slightly downvalley. Valleys surrounding the caldera of Apollinaris appear to have formed by fluvial and possibly by volcanic density flow processes, while those on the Apollinaris fan structure may have a mixed lava and fluvial origin. Valleys on Tyrrhena have broad flat floors and theater heads, which have been extensively enlarged, probably by sapping.

  7. OBSERVED AIR POLLUTION SPECIFICS OF THE VALLEY-BASED TOWNS

    Directory of Open Access Journals (Sweden)

    ZOLTÁN UTASI

    2012-03-01

    Full Text Available Observed air pollution specifics of the valley-based towns. There are 21 valley-based towns in the 100 most populated ones of Hungary. This topographical feature may be advantageous due to mezoscale circulation between the valley or basin, containing these settlements and the surrounding hills. On the other hand, the hills form a mechanical obstacle that may limit the vertical mixing of pollution. Final result of these counteracting features are analysed by comparing air pollution characteristics of these settlements with those in two control groups, also counting 21-21 non-valley towns of the country. Each group represents slightly over 1 million inhabitants. Nitrogen dioxide (NO2, sulphur-dioxide (SO2 concentrations and the total particulate matter below 10 µm (PM10 are considered. Annual mean, maxima and median values are analysed in 2007. For NO2 the valley-based towns are slightly cleaner, whereas for SO2 the situation is the opposite. The PM10 values do not indicate much difference between valley based and fully plain settlements. For explanation of these results, selected settlement and communal statistics are also compared. The paper is terminated by a sub-collection of similar valley-based towns in Central Europe.

  8. Geothermal hydrology of Warner Valley, Oregon: a reconnaissance study

    Energy Technology Data Exchange (ETDEWEB)

    Sammel, E.A.; Craig, R.W.

    1981-01-01

    Warner Valley and its southern extension, Coleman Valley, are two of several high-desert valleys in the Basin and Range province of south-central Oregon that contain thermal waters. At least 20 thermal springs, defined as having temperatures of 20/sup 0/C or more, issue from Tertiary basaltic flows and tuffs in and near the valleys. Many shallow wells also produce thermal waters. The highest measured temperature is 127/sup 0/C, reported from a well known as Crump geyser, at a depth of 200 meters. The hottest spring, located near Crump geyser, has a surface temperature of 78/sup 0/C. The occurrence of these thermal waters is closely related to faults and fault intersections in the graben and horst structure of the valleys. Chemical analyses show that the thermal waters are of two types: sodium chloride and sodium bicarbonate waters. Chemical indicators show that the geothermal system is a hot-water rather than a vapor-dominated system. Conductive heat flow in areas of the valley unaffected by hydrothermal convection is probably about 75 milliwatts per square meter. The normal thermal gradient in valley-fill dpeosits in these areas may be about 40/sup 0/C per kilometer. Geothermometers and mixing models indicate that temperatures of equilibration are at least 170/sup 0/C for the thermal components of the hotter waters. The size and location of geothermal reservoirs are unknown.

  9. Statistical Characterization of the Flow Structure in the Rhine Valley

    Science.gov (United States)

    Philippe, Philippe; Debas, Alain M.; Haeberli, Christian; Flamant, Pierre H.

    The flow structure at the intersection between the Rhine and the Seez valleys nearthe Swiss city of Bad Ragaz has been documented by means of wind and pressuremeasurements collected from 9 September to 10 November 1999 during the MesoscaleAlpine Programme (MAP) experiment. To understand better the dynamics of theageostrophic winds that develop in this part of the Rhine valley, some key questionsare answered in this paper including the following: (i) How does air blow at theintersection of the Rhine and Seez valleys? and (ii) what are the dynamical processes(mechanical or thermal) driving the flow circulations in the valleys?Statistical analysis of the wind and pressure patterns at synoptic scale and at the scaleof the valley shows that five main flow patterns, SE/S, NW/W, NW/N, NW/S, SE/N(wind direction in the Seez valley/wind direction in the Rhine valley) prevail. The SE/S regime is the flow splitting situation. It is mainly driven by a strong pressure gradient across the Alps leading to foehn, even though some nocturnal cases are generated bylocal thermal gradients. The NW/W and NW/N regimes are mechanically forced bythe synoptic pressure gradient (as the flow splitting case). The difference between thetwo regimes is due to the synoptic flow direction [westerly (northerly) synoptic flowfor the NW/W (NW/N) regime], showing that the Rhine valley (particularly from BadRagaz to Lake Constance) is less efficient in channelling the flow than the Seez valley.The NW/S (occurring mainly during nighttime) and SE/N (occurring mainly duringdaytime) regimes are mainly katabatic flows. However, the SE/N regime is also partlyforced at the synoptic scale during the foehn case that occurred between 18 October and 20 October 1999, with a complex layered vertical structure.

  10. A 1-D morphodynamic model of postglacial valley incision

    Science.gov (United States)

    Tunnicliffe, Jon F.; Church, Michael

    2015-11-01

    Chilliwack River is typical of many Cordilleran valley river systems that have undergone dramatic Holocene degradation of valley fills that built up over the course of Pleistocene glaciation. Downstream controls on base level, mainly blockage of valleys by glaciers, led to aggradation of significant glaciofluvial and glaciolacustrine valley fills and fan deposits, subsequently incised by fluvial action. Models of such large-scale, long-term degradation present a number of important challenges since the evolution of model parameters, such as the rate of bedload transport and grain size characteristics, are governed by the nature of the deposit. Sediment sampling in the Chilliwack Valley reveals a complex sequence of very coarse to fine textural modes. We present a 1-D numerical morphodynamic model for the river-floodplain system tailored to conditions in the valley. The model is adapted to dynamically adjust channel width to optimize sediment transporting capacity and to integrate relict valley fill material as the channel incises through valley deposits. Sensitivity to model parameters is studied using four principal criteria: profile concavity, rate of downstream grain size fining, bed surface sand content, and the timescale to equilibrium. Model results indicate that rates of abrasion and coarsening of the grain size distributions exert the strongest controls on all of the interrelated model performance criteria. While there are a number of difficulties in satisfying all model criteria simultaneously, results indicate that 1-D models of valley bottom sedimentary systems can provide a suitable framework for integrating results from sediment budget studies and chronologies of sediment evacuation established from dating.

  11. Groundwater availability of the Central Valley Aquifer, California

    Science.gov (United States)

    Faunt, Claudia C.

    2009-01-01

    California's Central Valley covers about 20,000 square miles and is one of the most productive agricultural regions in the world. More than 250 different crops are grown in the Central Valley with an estimated value of $17 billion per year. This irrigated agriculture relies heavily on surface-water diversions and groundwater pumpage. Approximately one-sixth of the Nation's irrigated land is in the Central Valley, and about one-fifth of the Nation's groundwater demand is supplied from its aquifers. The Central Valley also is rapidly becoming an important area for California's expanding urban population. Since 1980, the population of the Central Valley has nearly doubled from 2 million to 3.8 million people. The Census Bureau projects that the Central Valley's population will increase to 6 million people by 2020. This surge in population has increased the competition for water resources within the Central Valley and statewide, which likely will be exacerbated by anticipated reductions in deliveries of Colorado River water to southern California. In response to this competition for water, a number of water-related issues have gained prominence: conservation of agricultural land, conjunctive use, artificial recharge, hydrologic implications of land-use change, and effects of climate variability. To provide information to stakeholders addressing these issues, the USGS Groundwater Resources Program made a detailed assessment of groundwater availability of the Central Valley aquifer system, that includes: (1) the present status of groundwater resources; (2) how these resources have changed over time; and (3) tools to assess system responses to stresses from future human uses and climate variability and change. This effort builds on previous investigations, such as the USGS Central Valley Regional Aquifer System and Analysis (CV-RASA) project and several other groundwater studies in the Valley completed by Federal, State and local agencies at differing scales. The

  12. VALMET: a valley air pollution model. Final report. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Whiteman, C.D.; Allwine, K.J.

    1985-04-01

    An air quality model is described for predicting air pollution concentrations in deep mountain valleys arising from nocturnal down-valley transport and diffusion of an elevated pollutant plume, and the fumigation of the plume on the valley floor and sidewalls after sunrise. Included is a technical description of the model, a discussion of the model's applications, the required model inputs, sample calculations and model outputs, and a full listing of the FORTRAN computer program. 55 refs., 27 figs., 6 tabs.

  13. rOv-ASP-1, a recombinant secreted protein of the helminth Onchocercavolvulus, is a potent adjuvant for inducing antibodies to ovalbumin, HIV-1 polypeptide and SARS-CoV peptide antigens.

    Science.gov (United States)

    MacDonald, Angus J; Cao, Long; He, Yuxian; Zhao, Qian; Jiang, Shibo; Lustigman, Sara

    2005-05-16

    We studied the adjuvanticity of recombinant Onchocerca volvulus activation associated protein-1 (rOv-ASP-1) for ovalbumin (OVA) in mice. After a single immunization and one boost, rOv-ASP-1 exceeded the efficacy of alum or MPL + TDM adjuvants in terms of end-point total IgG or IgG1 and IgG2a anti-OVA titres. Using the helminth-derived adjuvant, IgG isotype responses to OVA were of a mixed Th1/Th2 profile and spleen cell cytokines exclusively Th1-type. The potent adjuvanticity of rOv-ASP-1 was confirmed in mice vaccinated with a 37-mer peptide from the S protein of SARS-CoV and an HIV-1 gp120-CD4 chimeric polypeptide antigen. Unusually for a helminth product, the rOv-ASP-1 adjuvant augmented not only Th2 but also Th1 responses, the latter property being of potential utility in stimulating anti-viral immune responses.

  14. Demonstration of using quieter pavement in Death Valley National Park

    Science.gov (United States)

    2013-06-01

    Death Valley National Park provided an environment that allowed a demonstration of : quieter pavement use. Sound measurements near the tire-pavement interface, near the : road, and in areas of frequent human use were conducted and analyses performed ...

  15. Bird Use of Imperial Valley Crops [ds427

    Data.gov (United States)

    California Natural Resource Agency — Agriculture crops in the Imperial Valley of California provide valuable habitat for many resident and migratory birds and are a very important component of the...

  16. Spin-valley splitting of electron beam in graphene

    Directory of Open Access Journals (Sweden)

    Yu Song

    2016-11-01

    Full Text Available We study spatial separation of the four degenerate spin-valley components of an electron beam in a EuO-induced and top-gated ferromagnetic/pristine/strained graphene structure. We show that, in a full resonant tunneling regime for all beam components, the formation of standing waves can lead sudden phase jumps ∼−π and giant lateral Goos-Hänchen shifts as large as the transverse beam width, while the interplay of the spin and valley imaginary wave vectors in the modulated regions can lead differences of resonant angles for the four spin-valley flavors, manifesting a spin-valley beam splitting effect. The splitting effect is found to be controllable by the gating and strain.

  17. Chinook Critical Habitat, Central Valley - NOAA [ds125

    Data.gov (United States)

    California Department of Resources — This layer depicts areas designated for Chinook Critical Habitat as well as habitat type and quality in the Central Valley Spring-run Evolutionary Significant Unit...

  18. Steelhead Critical Habitat, Central Valley - NOAA [ds123

    Data.gov (United States)

    California Natural Resource Agency — This layer depicts areas designated for Steelhead Critical Habitat as well as habitat type and quality in the California Central Valley Evolutionary Significant Unit...

  19. San Joaquin Valley Aerosol Health Effects Research Center (SAHERC)

    Data.gov (United States)

    Federal Laboratory Consortium — At the San Joaquin Valley Aerosol Health Effects Center, located at the University of California-Davis, researchers will investigate the properties of particles that...

  20. Factors influencing immunisation coverage in Mathare Valley, Nairobi

    African Journals Online (AJOL)

    : Cross section destrictive study. Setting: Mathare valley slums in Central district of Nairobi, Kenya. Subjects: Seven hundred and twelve children aged 12-23 months. Results: Access to immunisation services was excellent at 95.6%. However ...

  1. West Valley Reprocessing Plant. Safety analysis report, supplement 21

    International Nuclear Information System (INIS)

    1976-01-01

    Supplement No. 21 contains responses to USNRC questions on quality assurance contained in USNRC letter to NFS dated January 22, 1976, revised pages for the safety analysis report, and Appendix IX ''Quality Assurance Manual--West Valley Construction Projects.''

  2. Comparison of access to medicines between Klang Valley and East ...

    African Journals Online (AJOL)

    income of USD1/person/day) between urbanised Klang Valley and rural East Coast of Peninsular Malaysia. Methods: A semi-structured interview was conducted with caregivers to determine demographics, access to medicines, knowledge, ...

  3. Gravity and magnetic data of Midway Valley, southwest Nevada

    International Nuclear Information System (INIS)

    Ponce, D.A.; Langenheim, V.E.; Sikora, R.F.

    1993-01-01

    Detailed gravity and ground magnetic data collected along five traverses across Midway Valley on the eastern flank of Yucca Mountain in southwest Nevada are described. These data were collected as part of an effort to evaluate faulting in the vicinity of proposed surface facilities for a potential nuclear waste repository at Yucca Mountain. Geophysical data show that Midway Valley is bounded by large gravity and magnetic anomalies associated with the Bow Ridge and Paintbrush Canyon faults, on the west side of Exile Hill and on the west flank of Fran Ridge, respectively. In addition, Midway Valley itself is characterized by a number of small-amplitude anomalies that probably reflect small-scale faulting beneath Midway Valley

  4. Comparison of access to medicines between Klang Valley and East ...

    African Journals Online (AJOL)

    income of. USD1/person/day) between ... medicines for their children such as financial, transportation, physical and attitudinal. Conclusion: Access to medicines for ..... some caregivers felt that public transportation in the Klang Valley was not ...

  5. Sociocultural and economic dimensions of Rift Valley fever.

    Science.gov (United States)

    Muga, Geoffrey Otieno; Onyango-Ouma, Washington; Sang, Rosemary; Affognon, Hippolyte

    2015-04-01

    Health researchers have advocated for a cross-disciplinary approach to the study and prevention of infectious zoonotic diseases, such as Rift Valley Fever. It is believed that this approach can help bring out the social determinants and effects of the zoonotic diseases for the design of appropriate interventions and public health policy. A comprehensive literature review using a systematic search strategy was undertaken to explore the sociocultural and economic factors that influence the transmission and spread of Rift Valley Fever. Although the findings reveal a paucity of social research on Rift Valley Fever, they suggest that livestock sacrificial rituals, food preparation and consumption practices, gender roles, and inadequate resource base for public institutions are the key factors that influence the transmission. It is concluded that there is need for cross-disciplinary studies to increase the understanding of Rift Valley Fever and facilitate appropriate and timely response and mitigation measures. © The American Society of Tropical Medicine and Hygiene.

  6. Rock glacier inventory, Printse Valley, Valais, Switzerland, Version 1

    Data.gov (United States)

    National Aeronautics and Space Administration — The Printse Valley is a 16-km-long north-orientated basin. The south-part (summits at 300 m asl) is glacierized. The intermediate sector, more continental, is very...

  7. 27 CFR 9.66 - Russian River Valley.

    Science.gov (United States)

    2010-04-01

    ... Springs map. (22) Proceed 4.8 miles north-northwest along Mark West Springs Road, which becomes Porter Creek Road, to its intersection with Franz Valley Road, a light-duty road to the north of Porter Creek...

  8. Vernal Pool Complexes - Central Valley, 1989-1998 [ds36

    Data.gov (United States)

    California Natural Resource Agency — This Arc/Info coverage is a polygon layer of vernal pool complexes greater than 40 acres in size for 29 counties throughout the greater Central Valley, and some...

  9. NPP Tropical Forest: Magdalena Valley, Colombia, 1970-1971

    Data.gov (United States)

    National Aeronautics and Space Administration — ABSTRACT: Biomass, litterfall, and nutrient content of above-ground vegetation and soil for a tropical seasonal evergreen forest at Magdalena Valley, Columbia,...

  10. Physiologically based pharmacokinetic modeling of 18F-SiFAlin-Asp3-PEG1-TATE in AR42J tumor bearing mice

    International Nuclear Information System (INIS)

    Maaß, Christian; Rivas, Jose Ricardo Avelar; Attarwala, Ali Asgar; Hardiansyah, Deni; Niedermoser, Sabrina; Litau, Shanna; Wängler, Carmen; Wängler, Björn; Glatting, Gerhard

    2016-01-01

    Purpose: Peptide receptor radionuclide therapy (PRRT) is commonly performed in the treatment of neuroendocrine tumors (NET), where somatostatin analogs (DOTATATE) are radiolabeled with 90 Y, 68 Ga or 111 In for pre-therapeutic and therapeutic purposes. Quantitative evaluation of the biokinetic data can be performed by using physiologically based pharmacokinetic (PBPK) models. Knowledge about the biodistribution in a pre-clinical setting would allow optimizing the translation from bench to bedside. The aim of this study was to develop a PBPK model to describe the biodistribution of a novel sst2-targeting radiotracer. Methods: Biokinetic data of six mice after injection of 18 F-SiFAlin-Asp 3 -PEG 1 -TATE were investigated using two PBPK models. The PBPK models describe the biodistribution of the tracer in the tumor, kidneys, liver, remainder and whole body via blood flow to these organs via absorption, distribution, metabolism and excretion. A recently published sst2 PBPK model for humans (model 1) was used to describe the data. Physiological information in this model was adapted to that of a mouse. Model 1 was further modified by implementing receptor-mediated endocytosis (model 2). Model parameters were fitted to the biokinetic data of each mouse. Model selection was performed by calculating Akaike weights w i using the corrected Akaike Information Criterion (AICc). Results: The implementation of receptor-mediated endocytosis considerably improved the description of the biodistribution (Akaike weights w 1 = 0% and w 2 = 100% for model 1 and 2, respectively). The resulting time-integrated activity coefficients determined by model 2 were for tumor (0.05 ± 0.02) h, kidneys (0.11 ± 0.01) h and liver (0.02 ± 0.01) h. Conclusion: Simply downscaling a human PBPK model does not allow for an accurate description of 18 F-SiFAlin-Asp 3 -PEG 1 -TATE in mice. Biokinetics of this tracer can be accurately and adequately described using a physiologically based pharmacokinetic

  11. Seismicity related to geothermal development in Dixie Valley, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Ryall, A.S.; Vetter, U.R.

    1982-07-08

    A ten-station seismic network was operated in and around the Dixie Valley area from January 1980 to November 1981; three of these stations are still in operation. Data from the Dixie Valley network were analyzed through 30 Jun 1981, and results of analysis were compared with analysis of somewhat larger events for the period 1970-1979. The seismic cycle in the Western Great Basic, the geologic structural setting, and the instrumentation are also described.

  12. Proximity to citrus influences Pierce's disease in Temecula Valley vineyards

    OpenAIRE

    Perring, Thomas M.; Farrar, Charles A.; Blua, Matthew

    2001-01-01

    Pierce's disease has caused extensive losses to grapes in the Temecula Valley. The primary vector of Pierce's disease in the region is the glassy-winged sharpshooter (GWSS), which has been found in large numbers in citrus trees. We examined the role of citrus in the Temecula Valley Pierce's disease epidemic and found that citrus groves have influenced the incidence and severity of Pierce's disease in grapes. Because GWSS inhabit citrus in large numbers, California grape growers should take ad...

  13. Topological Valley Transport at Bilayer Graphene Domain Walls

    Science.gov (United States)

    2015-04-22

    resistances are lower than that expected from the semiconductor bandgap in ideal bilayer graphene , presumably owing to impurities and defects in our devices...LETTER doi:10.1038/nature14364 Topological valley transport at bilayer graphene domain walls Long Ju1*, Zhiwen Shi1*, Nityan Nair1, Yinchuan Lv1...Electron valley, a degree of freedom that is analogous to spin, can lead to novel topological phases in bilayer graphene . A tunable bandgap can be

  14. Inca expansion and parasitism in the Lluta Valley: preliminary data

    OpenAIRE

    Santoro Calogero; Vinton Sheila Dorsey; Reinhard Karl J

    2003-01-01

    Assessing the impact of cultural change on parasitism has been a central goal in archaeoparasitology. The influence of civilization and the development of empires on parasitism has not been evaluated. Presented here is a preliminary analysis of the change in human parasitism associated with the Inca conquest of the Lluta Valley in Northern Chile. Changes in parasite prevalence are described. It can be seen that the change in life imposed on the inhabitants of the Lluta Valley by the Incas cau...

  15. Hydrothermal system in Southern Grass Valley, Pershing County, Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Welch, A.H.; Sorey, M.L.; Olmsted, F.H.

    1981-01-01

    Southern Grass Valley is a fairly typical extensional basin in the Basin and Range province. Leach Hot Springs, in the southern part of the valley, represents the discharge end of an active hydrothermal flow system with an estimated deep aquifer temperature of 163 to 176/sup 0/C. Results of geologic, hydrologic, geophysical and geochemical investigations are discussed in an attempt to construct an internally consistent model of the system.

  16. Makran Mountain Range, Indus River Valley, Pakistan, India

    Science.gov (United States)

    1984-01-01

    The enormous geologic pressures exerted by continental drift can be very well illustrated by the long northward curving parallel folded mountain ridges and valleys of the coastal Makran Range of Pakistan (27.0N, 66.0E). As a result of the collision of the northward bound Indian sub-continent into the Asian Continent, the east/west parallel range has been bent in a great northward arc and forming the Indus River valley at the interface of the collision.

  17. Effects of Cache Valley Particulate Matter on Human Lung Cells

    OpenAIRE

    Watterson, Todd L.

    2012-01-01

    During wintertime temperature inversion episodes the concentrations of particulate air pollution, also defined as particulate matter (PM), in Utah’s Cache Valley have often been highest in the nation, with concentrations surpassing more populated and industrial areas. This has attracted much local and national attention to the area and its pollution. The Cache Valley has recently been declared to be in non-attainment of provisions of Federal law bringing to bear Federal regulatory attention a...

  18. Reconstruction of the MSRs in-situ at Beaver Valley

    International Nuclear Information System (INIS)

    Yarden, A.; Tam, C.W.; Deahna, S.T.; McFeaters, C.V.

    1992-01-01

    The Moisture Separator Reheaters (MSRs) have been problem components at Beaver Valley 1 pressurized water reactor since the plant started up 16 years ago, many of the problems encountered being widespread in the nuclear industry. In 1991, Duquesne Light rebuilt the Beaver Valley 1 MSRs and in 1992 did the same at unit 2. The reconstruction projects have proved cost effective with short payback times and significant improvements in station performance. (Author)

  19. Vitrification process equipment design for the West Valley Demonstration Project

    International Nuclear Information System (INIS)

    Chapman, C.C.; Drosjack, W.P.

    1988-10-01

    The vitrification process and equipment design is nearing completion for the West Valley Project. This report provides the basis and current status for the design of the major vessels and equipment within the West Valley Vitrification Plant. A review of the function and key design features of the equipment is also provided. The major subsystems described include the feed preparation and delivery systems, the melter, the canister handling systems, and the process off-gas system. 11 refs., 33 figs., 4 tabs

  20. Why do European companies have Innovation Hubs in Silicon Valley

    DEFF Research Database (Denmark)

    Berger, Andreas; Brem, Alexander

    2017-01-01

    Innovation hubs are gaining high attention in recent years, especially from European companies. Silicon Valley has been deemed as one of the most attractive and successful environments for establishing innovation hubs. This article highlights examples of companies from Europe that made the step...... to California—namely, Volkswagen, Swisscom, BMW, Axel Springer, Munich Re, and Innogy SE (RWE Group). Based on these companies’ experiences, recommendations are given on how companies might approach a setup in Silicon Valley for long-term success....

  1. Quaternary glaciation of the Tashkurgan Valley, Southeast Pamir

    Science.gov (United States)

    Owen, Lewis A.; Chen, Jie; Hedrick, Kathyrn A.; Caffee, Marc W.; Robinson, Alexander C.; Schoenbohm, Lindsay M.; Yuan, Zhaode; Li, Wenqiao; Imrecke, Daniel B.; Liu, Jinfeng

    2012-07-01

    The Quaternary glacial history of Tashkurgan valley, in the transition between the Pamir and Karakoram, in Xinjiang Province, China was examined using remote sensing, field mapping, geomorphic analysis of landforms and sediments, and 10Be terrestrial cosmogenic nuclide dating. Moraines were assigned to four glacial stages: 1) the Dabudaer glacial stage that dates to the penultimate glacial cycle and/or earlier, and may represent one or more glaciations; 2) the Tashkurgan glacial stage that dates to early last glacial, most likely Marine Oxygen Isotope Stage (MIS) 4; 3) the Hangdi glacial stage that dates to MIS 2, possibly early MIS 2; and 4) the Kuzigun glacial stage that dates to the MIS 2, possibly the global Last Glacial Maximum, and is younger than the Hangdi glacial stage. Younger moraines and rock glaciers are present at the heads of tributary valleys; but these were inaccessible because they are located close to politically sensitive borders with Pakistan, Afghanistan and Tajikistan. Glaciers during the Dabudaer glacial stage advanced into the central part of the Tashkurgan valley. During the Tashkurgan glacial stages, glaciers advanced several kilometers beyond the mouths of the tributary valleys into the Tashkurgan valley. Glaciers during the Hangdi and Kuzigun glacial stages advanced just beyond the mouths of the tributary valleys. Glaciation in this part of the Himalayan-Tibetan orogen is likely strongly controlled by northern hemisphere climate oscillations, although a monsoonal influence on glaciation cannot be ruled out entirely.

  2. Geothermal resource assessment of western San Luis Valley, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Zacharakis, Ted G.; Pearl, Richard Howard; Ringrose, Charles D.

    1983-01-01

    The Colorado Geological Survey initiated and carried out a fully integrated assessment program of the geothermal resource potential of the western San Luis Valley during 1979 and 1980. The San Luis Valley is a large intermontane basin located in southcentral Colorado. While thermal springs and wells are found throughout the Valley, the only thermal waters found along the western part of the Valley are found at Shaw Warm Springs which is a relatively unused spring located approximately 6 miles (9.66 km) north of Del Norte, Colorado. The waters at Shaws Warm Spring have a temperature of 86 F (30 C), a discharge of 40 gallons per minute and contain approximately 408 mg/l of total dissolved solids. The assessment program carried out din the western San Luis Valley consisted of: soil mercury geochemical surveys; geothermal gradient drilling; and dipole-dipole electrical resistivity traverses, Schlumberger soundings, Audio-magnetotelluric surveys, telluric surveys, and time-domain electro-magnetic soundings and seismic surveys. Shaw Warm Springs appears to be the only source of thermal waters along the western side of the Valley. From the various investigations conducted the springs appear to be fault controlled and is very limited in extent. Based on best evidence presently available estimates are presented on the size and extent of Shaw Warm Springs thermal system. It is estimated that this could have an areal extent of 0.63 sq. miles (1.62 sq. km) and contain 0.0148 Q's of heat energy.

  3. Ventilation potential during the emissions survey in Toluca Valley, Mexico

    Science.gov (United States)

    Ruiz Angulo, A.; Peralta, O.; Jurado, O. E.; Ortinez, A.; Grutter de la Mora, M.; Rivera, C.; Gutierrez, W.; Gonzalez, E.

    2017-12-01

    During the late-spring early-summer measurements of emissions and pollutants were carried out during a survey campaign at four different locations within the Toluca Valley. The current emissions inventory typically estimates the generation of pollutants based on pre-estimated values representing an entire sector function of their activities. However, those factors are not always based direct measurements. The emissions from the Toluca Valley are rather large and they could affect the air quality of Mexico City Valley. The air masses interchange between those two valleys is not very well understood; however, based on the measurements obtained during the 3 months campaign we looked carefully at the daily variability of the wind finding a clear signal for mountain-valley breeze. The ventilation coefficient is estimated and the correlations with the concentrations at the 4 locations and in a far away station in Mexico City are addressed in this work. Finally, we discuss the implication of the ventilation capacity in air quality for the system of Valleys that include Mexico City.

  4. Valley-selective optical Stark effect probed by Kerr rotation

    Science.gov (United States)

    LaMountain, Trevor; Bergeron, Hadallia; Balla, Itamar; Stanev, Teodor K.; Hersam, Mark C.; Stern, Nathaniel P.

    2018-01-01

    The ability to monitor and control distinct states is at the heart of emerging quantum technologies. The valley pseudospin in transition metal dichalcogenide (TMDC) monolayers is a promising degree of freedom for such control, with the optical Stark effect allowing for valley-selective manipulation of energy levels in WS2 and WSe2 using ultrafast optical pulses. Despite these advances, understanding of valley-sensitive optical Stark shifts in TMDCs has been limited by reflectance-based detection methods where the signal is small and prone to background effects. More sensitive polarization-based spectroscopy is required to better probe ultrafast Stark shifts for all-optical manipulation of valley energy levels. Here, we show time-resolved Kerr rotation to be a more sensitive probe of the valley-selective optical Stark effect in monolayer TMDCs. Compared to the established time-resolved reflectance methods, Kerr rotation is less sensitive to background effects. Kerr rotation provides a fivefold improvement in the signal-to-noise ratio of the Stark effect optical signal and a more precise estimate of the energy shift. This increased sensitivity allows for observation of an optical Stark shift in monolayer MoS2 that exhibits both valley and energy selectivity, demonstrating the promise of this method for investigating this effect in other layered materials and heterostructures.

  5. Assessment of Wastewater in Duhok Valley, Kurdistan Region/Iraq

    Directory of Open Access Journals (Sweden)

    Najmaldin E. Hassan

    2016-08-01

    Full Text Available In order to characterize the waste water in Duhok valley in Duhok governorate, during 25km, seven sites were selected in Duhok valley, to represent their water quality. Monthly samples were collected from the Duhok valley for the period from, April to September, 2015. The qualitative study of Duhok valley water tested, as considered one of the main sources of water pollution for Musol Lake. The physical and chemical test for water samples are taken from different locations in Duhok valley. To know the degree of pollution, and the impact of self-purification processes to improve water quality before arriving to the Mosul Lake, and the indicated results of the study a lack of dissolved oxygen in the water (DO. And high organic load values, (BOD and most of the bad qualities during water passage within the city of Duhok, while meat a significant improvement in the quality of water downstream before arriving at the dam Lake, is attributed to the effect of operations of self- purification ability of water. In spite of salinity problems and toxicity, the quality of water is suitable for irrigation crops on both sides of the valley .The all samples were tested for conductivity, TDS, pH, total hardness, chloride, alkalinity, sulfate, BOD, and phosphate, according to the standard methods.

  6. Higgs portal valleys, stability and inflation

    Energy Technology Data Exchange (ETDEWEB)

    Ballesteros, Guillermo [Instituto de Física Teórica IFT-UAM/CSIC,C/ Nicolás Cabrera 13-15, Cantoblanco, 28049 Madrid (Spain); CERN, Theory Division,1211 Geneva (Switzerland); Tamarit, Carlos [Institute for Particle Physics Phenomenology, Durham University,South Road, Durham, DH1 3LE (United Kingdom)

    2015-09-30

    The measured values of the Higgs and top quark masses imply that the Standard Model potential is very likely to be unstable at large Higgs values. This is particularly problematic during inflation, which sources large perturbations of the Higgs. The instability could be cured by a threshold effect induced by a scalar with a large vacuum expectation value and directly connected to the Standard Model through a Higgs portal coupling. However, we find that in a minimal model in which the scalar generates inflation, this mechanism does not stabilize the potential because the mass required for inflation is beyond the instability scale. This conclusion does not change if the Higgs has a direct weak coupling to the scalar curvature. On the other hand, if the potential is absolutely stable, successful inflation in agreement with current CMB data can occur along a valley of the potential with a Mexican hat profile. We revisit the stability conditions, independently of inflation, and clarify that the threshold effect cannot work if the Higgs portal coupling is too small. We also show that inflation in a false Higgs vacuum appearing radiatively for a tuned ratio of the Higgs and top masses leads to an amplitude of primordial gravitational waves that is far too high, ruling out this possibility.

  7. Tennessee Valley Region: a year 2000 profile

    International Nuclear Information System (INIS)

    1978-06-01

    A study was undertaken to determine the potential radiological implications of nuclear facilities in the combined watersheds of the Tennessee and Cumberland rivers, an area covering portions of 7 states of varied topography. The regional population in 1970 was about 4.6 million and is expected to increase to about 7 million by the year 2000. A 1973 projection estimated the installed electric generating capacity of the region to increase from a 1970 value of 45,000 megawatts to a total of 222,000 megawatts by the year 2000. In that year, about 144,000 megawatts were projected to be nuclear plants. The profile of the Tennessee Valley Region in the year 2000, as drawn from this report, contains the essential data for calculation of the radiological dose from operation of nuclear facilities in that year. Those calculations are reported in the companion document, DOE/ET-0064/2. Specifically, Volume I establishes the parameters describing where the people live, what they eat, the activities in which they engage, and the environmental surroundings that enable an evaluation of the potential radiation dose to the population. Airborne radionuclides from nuclear facilities in this zone may enter the study area and be deposited on the ground, on growing food, and on water surfaces. Consideration was not given to waterborne radionuclides external to the study region. 17 references

  8. Tennessee Valley Region: a year 2000 profile

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-06-01

    A study was undertaken to determine the potential radiological implications of nuclear facilities in the combined watersheds of the Tennessee and Cumberland rivers, an area covering portions of 7 states of varied topography. The regional population in 1970 was about 4.6 million and is expected to increase to about 7 million by the year 2000. A 1973 projection estimated the installed electric generating capacity of the region to increase from a 1970 value of 45,000 megawatts to a total of 222,000 megawatts by the year 2000. In that year, about 144,000 megawatts were projected to be nuclear plants. The profile of the Tennessee Valley Region in the year 2000, as drawn from this report, contains the essential data for calculation of the radiological dose from operation of nuclear facilities in that year. Those calculations are reported in the companion document, DOE/ET-0064/2. Specifically, Volume I establishes the parameters describing where the people live, what they eat, the activities in which they engage, and the environmental surroundings that enable an evaluation of the potential radiation dose to the population. Airborne radionuclides from nuclear facilities in this zone may enter the study area and be deposited on the ground, on growing food, and on water surfaces. Consideration was not given to waterborne radionuclides external to the study region. 17 references. (MCW)

  9. Higgs portal valleys, stability and inflation

    CERN Document Server

    Ballesteros, Guillermo

    2015-01-01

    The measured values of the Higgs and top quark masses imply that the Standard Model potential is very likely to be unstable at large Higgs values. This is particularly problematic during inflation, which sources large perturbations of the Higgs. The instability could be cured by a threshold effect induced by a scalar with a large vacuum expectation value and directly connected to the Standard Model through a Higgs portal coupling. However, we find that in a minimal model in which the scalar generates inflation, this mechanism does not stabilize the potential because the mass required for inflation is beyond the instability scale. This conclusion does not change if the Higgs has a direct weak coupling to the scalar curvature. On the other hand, if the potential is absolutely stable, successful inflation in agreement with current CMB data can occur along a valley of the potential with a Mexican hat profile. We revisit the stability conditions, independently of inflation, and clarify that the threshold effect ca...

  10. Flowers of Çoruh Valley

    Directory of Open Access Journals (Sweden)

    Ramazan Çakmakçı

    2018-01-01

    Full Text Available Coruh valley has an important biological diversity in term of plants, flora-fauna, wildlife and ecosystems. These regions contain the landraces, wild and weedy relatives, other wild, herbaceous and flowering trees, herbaceous flowering plants, medicinal and aromatic and flowering and ornamental shrubs plants species which are especially economically important plant for floriculture, eco-tourism, botanical tourism and nature tourism. Many important medicinal and aromatic and ornamental plants species are found in this region and naturally grow. It is considered that Acantholimon, Achillea, Alkanna, Allium, Amygdalus, Angelica, Anemone, Anthemis, Arabis, Arctium, Artemisia, Asparagus, Asperula, Astragalus, Calamintha, Calendula, Calutea, Campanula, Capparis, Cardamine, Centaurea, Cephalanthera, Cephalaria, Chelidonium, Chenopodium, Chysanthemum, Colchicum, Consolida, Coriandrum, Cornus, Coronilla, Cerasus, Cotoneaster, Crataegus, Crocus, Cyclamen, Dactylorhiza, Digitalis, Dianthus, Draba, Echinops, Equisetum, Ferula, Filipendula, Fritillaria, Fumaria, Gagea, Galanthus, Galium, Genista, Gentiana, Geranium, Geum, Gladiolus, Glychirrza, Helichrysum, Hesperis, Hypericum, İnula, İris, Isatis, Juniperus, Lilium, Linaria, Linum, lysimachia, Malus, Malva, Marrubium, Melissa, Mentha, Micromeria, Morina, Muscari, Mysotis, Narcissus, Neotchichatchewia, Nepeta, Onobrychis, Orchis, Ornithogalum, Origanum, Paeonia, Papaver, Pedicularis, Peganum, Phelypaea, Platanthera, Plantago, Pilosella, Pelargonium, Potentilla, Polygonum, Polygala, Primula, Punica, Prunus, Pyrus, Ranunculus, Rhamnus, Rhododendron, Rhus, Rosa, Rubia, Rubus, Rumex, Salvia, Sambucus, Satureja, Scilla, Scorzonera, Scutellaria, Sedum, Sempervivum, Sideritis, Sophora, Sorbus, Stachys, Tanecetum, Teucrium, Thymus, Trigonella, Tulipa, Tussilago, Uechtriitzia, Vaccinium, Verbascum, Verbena, Veronica, Viburnum and Ziziphora species commonly found in the region may be may be evaluated economically.

  11. Inflammatory events induced by Lys-49 and Asp-49 phospholipases A2 isolated from Bothrops asper snake venom: role of catalytic activity.

    Science.gov (United States)

    Zuliani, Juliana P; Fernandes, Cristina M; Zamuner, Stella R; Gutiérrez, José M; Teixeira, Catarina F P

    2005-03-01

    The inflammatory events induced in the peritoneal cavity of mice by two PLA2s isolated from Bothrops asper snake venom were investigated. MT-III, an Asp-49 catalytically active enzyme and MT-II, a catalytically inactive Lys-49 variant induced increase in vascular permeability. Inhibition of enzymatic activity of MT-III with p-bromophenacyl bromide reduced this effect. MT-III induced a larger neutrophil infiltrate than MT-II. This activity was significantly reduced after inhibition of catalytic activity. Reduction in the number of neutrophils was observed when antibodies against L-selectin, CD18 or LFA-1 were used, suggesting the involvement of these adhesion molecules in the effects of both PLA2s. There was no effect with antibodies against ICAM-1 and PECAM-1. Increase in the levels of LTB4 and TXA2, as well as of IL-1, IL-6 and TNF-alpha, were observed in the peritoneal exudates induced by MT-III. MT-II did not enhance levels of eicosanoids but increased those of cytokines. It is concluded that both PLA2s induce inflammatory events in this model. Since MT-III exerts a stronger proinflammatory effect, the enzymatic phospholipid hydrolysis may be relevant for these phenomena. However, the fact that MT-II induced inflammation suggests that molecular regions distinct from the catalytic site elicit inflammatory events perhaps by interacting with specific cell membrane acceptors.

  12. A NOVEL P.ASP304GLY MUTATION IN BEST1 GENE ASSOCIATED WITH ATYPICAL BEST VITELLIFORM MACULAR DYSTROPHY PHENOTYPE AND HIGH INTRAFAMILIAL VARIABILITY.

    Science.gov (United States)

    Peiretti, Enrico; Caminiti, Giulia; Forma, Gina; Carboni, Giovannella; Dhaenens, Claire-Marie; Querques, Lea; Souied, Eric; Querques, Giuseppe

    2016-09-01

    To report the atypical phenotypic characteristics of patients with a novel p.Asp304Gly mutation in BEST1. Affected individuals underwent a complete ophthalmic examination, including best-corrected visual acuity, fundus autofluorescence, spectral domain optical coherence tomography, and electrophysiologic testing. All individuals were screened for mutations in the BEST1 gene. Five patients of the same Italian family were clinically examined. All patients complained of decreased vision as the initial symptom. Best-corrected visual acuity ranged from 20/800 to 20/32. On fundus examination, all patients showed atypical Best vitelliform macular dystrophy phenotype with multifocal macular and extramacular involvement. The spectral domain optical coherence tomography characteristics of central macular and extramacular lesions varied in each patient and included "giant" choroidal excavation, extensive flat macular elevation with hyporeflective subretinal material accumulation surrounded by hyperautofluorescent spots/annulus, and extensive hypoautofluorescent extramacular atrophic areas. Electrooculogram was always abnormal with Arden ratio lower than 1.55, whereas electroretinogram was normal in the two younger patients and abnormal (low amplitude) in the three older patients. Genetic analysis revealed a novel missense mutation in BEST1, substituting aspartate for glycine at amino acid 304. We describe the atypical phenotype and high intrafamilial variability associated with a new mutation in the BEST1 gene in an Italian family affected with Best vitelliform macular dystrophy. Clinicians should consider screening the BEST1 gene even in the absence of the typical phenotype and in case of high intrafamilial variability.

  13. A Ser75-to-Asp phospho-mimicking mutation in Src accelerates ageing-related loss of retinal ganglion cells in mice.

    Science.gov (United States)

    Kashiwagi, Kenji; Ito, Sadahiro; Maeda, Shuichiro; Kato, Goro

    2017-12-01

    Src knockout mice show no detectable abnormalities in central nervous system (CNS) post-mitotic neurons, likely reflecting functional compensation by other Src family kinases. Cdk1- or Cdk5-dependent Ser75 phosphorylation in the amino-terminal Unique domain of Src, which shares no homology with other Src family kinases, regulates the stability of active Src. To clarify the roles of Src Ser75 phosphorylation in CNS neurons, we established two types of mutant mice with mutations in Src: phospho-mimicking Ser75Asp (SD) and non-phosphorylatable Ser75Ala (SA). In ageing SD/SD mice, retinal ganglion cell (RGC) number in whole retinas was significantly lower than that in young SD/SD mice in the absence of inflammation and elevated intraocular pressure, resembling the pathogenesis of progressive optic neuropathy. By contrast, SA/SA mice and wild-type (WT) mice exhibited no age-related RGC loss. The age-related retinal RGC number reduction was greater in the peripheral rather than the mid-peripheral region of the retina in SD/SD mice. Furthermore, Rho-associated kinase activity in whole retinas of ageing SD/SD mice was significantly higher than that in young SD/SD mice. These results suggest that Src regulates RGC survival during ageing in a manner that depends on Ser75 phosphorylation.

  14. Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms and typhoid susceptibility in Asian Malay population in Malaysia.

    Science.gov (United States)

    Bhuvanendran, Saatheeyavaane; Hussin, Hani M; Meran, Lila P; Anthony, Amy A; Zhang, Leilei; Burch, Lauranell H; Phua, Kia K; Ismail, Asma; Balaram, Prabha

    2011-09-01

    Typhoid fever is a major health problem with frequent outbreaks in Kelantan, Malaysia. Prevalence of TLR4 gene polymorphisms varies with ethnic groups (0-20%) and predisposean individual to gram-negative infections. The prevalence rate of TLR4 Asp299Gly and Thr399lle polymorphisms in the Malay population or the influence of these on typhoid fever susceptibility is not yet reported. 250 normal and 304 susceptible Malay individuals were investigated for these polymorphisms using allele-specific PCR and analysed for its association with typhoid fever susceptibility. The total prevalence of polymorphisms in the normal population was 4.8% in comparison to 12.5% in the susceptible population (p = 0.002). An increased frequency of both polymorphisms was observed in the susceptible population (p population and suggests that these polymorphisms confer a higher risk for typhoid, infection. The higher incidence of typhoid fever in Kelantan could be attributed to the higher percentage of Malays (95%) in this state. In order to reduce the incidence of this disease, people with these polymorphisms, can be prioritised for prophylactic strategies. Copyright © 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  15. Covalent attachment of cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) to poly(etheretherketone) surface by tailored silanization layers technique

    Science.gov (United States)

    Zheng, Yanyan; Xiong, Chengdong; Li, Xiaoyu; Zhang, Lifang

    2014-11-01

    Poly(etheretherketone) (PEEK) is a rigid semicrystalline polymer that combines excellent mechanical properties, broad chemical resistance and bone-like stiffness and is widely used in biomedical fields. However, PEEK is naturally bioinert, leading to limited biomedical applications, especially when a direct bone-implant osteointegration is desired. In this study, a three-step reaction procedure was employed to immobilize the cell-adhesive peptide Gly-Arg-Gly-Asp (GRGD) on the surface of PEEK sheet by covalent chemical attachment to favor cell adhesion and proliferation. First, hydroxylation-pretreated PEEK surfaces were silanized with 7-Oct-1-enyltrichlorosilane (OETS) in dry cyclohexane, resulting in a silanization layer with terminal ethenyl. Second, the terminal ethylenic double bonds of the silanization layer on PEEK surface were converted to carboxyl groups through acidic potassium manganate oxidation. Finally, GRGD was covalently attached by carbodiimide mediated condensation between the carboxyl on PEEK surface and amine presents in GRGD. X-ray photoelectron spectroscopy (XPS), attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, surface profiler and water contact angle measurements were applied to characterize the modified surfaces. The effect of cells attachment and proliferation on each specimen was investigated. Pre-osteoblast cells (MC3T3-E1) attachment, spreading and proliferation were improved effectively on GRGD-modified PEEK surface. PEEK modified with GRGD on its surface has potential use in orthopedic or dental implants.

  16. Identification of homozygous WFS1 mutations (p.Asp211Asn, p.Gln486*) causing severe Wolfram syndrome and first report of male fertility

    Science.gov (United States)

    Haghighi, Amirreza; Haghighi, Alireza; Setoodeh, Aria; Saleh-Gohari, Nasrollah; Astuti, Dewi; Barrett, Timothy G

    2013-01-01

    Wolfram syndrome (WFS) is a neurodegenerative genetic condition characterized by juvenile-onset of diabetes mellitus and optic atrophy. We studied clinical features and the molecular basis of severe WFS (neurodegenerative complications) in two consanguineous families from Iran. A clinical and molecular genetic investigation was performed in the affected and healthy members of two families. The clinical diagnosis of WFS was confirmed by the existence of diabetes mellitus and optic atrophy in the affected patients, who in addition had severe neurodegenerative complications. Sequencing of WFS1 was undertaken in one affected member from each family. Targeted mutations were tested in all members of relevant families. Patients had most of the reported features of WFS. Two affected males in the first family had fathered unaffected children. We identified two homozygous mutations previously reported with apparently milder phenotypes: family 1: c.631G>A (p.Asp211Asn) in exon 5, and family 2: c.1456C>T (p.Gln486*) in exon 8. Heterozygous carriers were unaffected. This is the first report of male Wolfram patients who have successfully fathered children. Surprisingly, they also had almost all the complications associated with WFS. Our report has implications for genetic counseling and family planning advice for other affected families. PMID:22781099

  17. Evaluation of Osteoblast-Like Cell Viability and Differentiation on the Gly-Arg-Gly-Asp-Ser Peptide Immobilized Titanium Dioxide Nanotube via Chemical Grafting.

    Science.gov (United States)

    Kim, Ga-Hyun; Kim, Il-Shin; Park, Sang-Won; Lee, Kwangmin; Yun, Kwi-Dug; Kim, Hyun-Seung; Oh, Gye-Jeong; Ji, Min-Kyung; Lim, Hyun-Pil

    2016-02-01

    This study examined the effect of the immobilization of the Gly-Arg-Gly-Asp-Ser (GRGDS) peptide on titanium dioxide (TiO2) nanotube via chemical grafting on osteoblast-like cell (MG-63) viability and differentiation. The specimens were divided into two groups; TiO2 nanotubes and GRGDS-immobilized TiO2 nanotubes. The surface characteristics of GRGDS-immobilized TiO2 nanotubes were observed by using X-ray photoelectron spectroscopy (XPS) and a field emission scanning electron microscope (FE-SEM). The morphology of cells on specimens was observed by FE-SEM after 2 hr and 24 hr. The level of cell viability was investigated via a tetrazolium (XTT) assay after 2 and 4 days. Alkaline phosphatase (ALP) activity was evaluated to measure the cell differentiation after 4 and 7 days. The presence of nitrogen up-regulation or C==O carbons con- firmed that TiO2 nanotubes were immobilized with GRGDS peptides. Cell adhesion was enhanced on the GRGDS-immobilized TiO2 nanotubes compared to TiO2 nanotubes. Furthermore, significantly increased cell spreading and proliferation were observed with the cells grown on GRGDS-immobilized TiO2 nanotubes (P nanotubes and TiO2 nanotubes. These results suggest that the GRGDS-immobilized TiO2 nanotubes might be effective in improving the osseointegration of dental implants.

  18. Comparison of the FXG™: RESP (Asp+) real-time PCR assay with direct immunofluorescence and calcofluor white staining for the detection of Pneumocystis jirovecii in respiratory specimens.

    Science.gov (United States)

    Seah, Christine; Richardson, Susan E; Tsui, George; Yu, Billy; Thornback, John; McTaggart, Lisa; Boggild, Andrea; Wengenack, Nancy L; Zhang, Sean X

    2012-04-01

    We compared the FXG™: RESP (Asp +) real-time PCR assay (Myconostica Ltd) with two microscopic staining methods (direct immunofluorescence [IFA] and calcofluor white) for the detection of Pneumocystis jirovecii in 411 respiratory specimens submitted for P. jirovecii examination. We considered the specimen to be microscopically positive if the organism could be visualized through the use of either IFA or calcofluor white. A second, published real-time PCR assay targeting the cdc2 gene of P. jirovecii was used to adjudicate those specimens that were microscopically negative but Myconostica PCR positive. The Myconostica PCR positive samples were deemed to be true positives if they were concordant with microscopically positive results or if they were positive by the second PCR assay. As a result, the Myconostica PCR assay was found to be more sensitive than the two microscopy methods in detecting P. jirovecii (10.5% true positivity rate by PCR, 8.0% by immunofluorescence, and 7.1% by calcofluor white). The Myconostica PCR assay showed 93.5% sensitivity, 95.1% specificity, 70.5% positive predictive value, and 99.1% negative predictive value. Its high negative predictive value suggests a role of the Myconostica PCR assay in ruling out Pneumocystis pneumonia.

  19. Effect of APE1 T2197G (Asp148Glu Polymorphism on APE1, XRCC1, PARP1 and OGG1 Expression in Patients with Colorectal Cancer

    Directory of Open Access Journals (Sweden)

    Juliana C. Santos

    2014-09-01

    Full Text Available It has been hypothesized that genetic variation in base excision repair (BER might modify colorectal adenoma risk. Thus, we evaluated the influence of APE1 T2197G (Asp148Glu polymorphism on APE1, XRCC1, PARP1 and OGG1 expression in normal and tumor samples from patients with colorectal cancer. The results indicate a downregulation of OGG1 and an upregulation of XRCC1 expression in tumor tissue. Regarding the anatomical location of APE1, OGG1 and PARP-1, a decrease in gene expression was observed among patients with cancer in the rectum. In patients with or without some degree of tumor invasion, a significant downregulation in OGG1 was observed in tumor tissue. Interestingly, when taking into account the tumor stage, patients with more advanced grades (III and IV showed a significant repression for APE1, OGG1 and PARP-1. XRCC1 expression levels were significantly enhanced in tumor samples and were correlated with all clinical and histopathological data. Concerning the polymorphism T2197G, GG genotype carriers exhibited a significantly reduced expression of genes of the BER repair system (APE1, XRCC1 and PARP1. In summary, our data show that patients with colorectal cancer present expression changes in several BER genes, suggesting a role for APE1, XRCC1, PARP1 and OGG1 and APE1 polymorphism in colorectal carcinogenesis.

  20. Identification of homozygous WFS1 mutations (p.Asp211Asn, p.Gln486*) causing severe Wolfram syndrome and first report of male fertility.

    Science.gov (United States)

    Haghighi, Amirreza; Haghighi, Alireza; Setoodeh, Aria; Saleh-Gohari, Nasrollah; Astuti, Dewi; Barrett, Timothy G

    2013-03-01

    Wolfram syndrome (WFS) is a neurodegenerative genetic condition characterized by juvenile-onset of diabetes mellitus and optic atrophy. We studied clinical features and the molecular basis of severe WFS (neurodegenerative complications) in two consanguineous families from Iran. A clinical and molecular genetic investigation was performed in the affected and healthy members of two families. The clinical diagnosis of WFS was confirmed by the existence of diabetes mellitus and optic atrophy in the affected patients, who in addition had severe neurodegenerative complications. Sequencing of WFS1 was undertaken in one affected member from each family. Targeted mutations were tested in all members of relevant families. Patients had most of the reported features of WFS. Two affected males in the first family had fathered unaffected children. We identified two homozygous mutations previously reported with apparently milder phenotypes: family 1: c.631G>A (p.Asp211Asn) in exon 5, and family 2: c.1456C>T (p.Gln486*) in exon 8. Heterozygous carriers were unaffected. This is the first report of male Wolfram patients who have successfully fathered children. Surprisingly, they also had almost all the complications associated with WFS. Our report has implications for genetic counseling and family planning advice for other affected families.