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Sample records for asparagus asparagus officinalis

  1. Major anthocyanins from purple asparagus (Asparagus officinalis).

    Science.gov (United States)

    Sakaguchi, Yumi; Ozaki, Yukio; Miyajima, Ikuo; Yamaguchi, Masaatsu; Fukui, Yuko; Iwasa, Keiko; Motoki, Satoru; Suzuki, Takashi; Okubo, Hiroshi

    2008-05-01

    Two major anthocyanins (A1 and A2) were isolated from peels of the spears of Asparagus officinalis cv. Purple Passion. They were purified by column, paper and high-performance liquid chromatographic separations, and their structures were elucidated by high-resolution Fourier transform ion cyclotron resonance mass spectrometry (HR-FT-ICR MS), 1H, 13C and two-dimensional NMR spectroscopic analyses and either acid or alkaline hydrolysis, respectively. A1 was identified as cyanidin 3-[3''-(O-beta-d-glucopyranosyl)-6''-(O-alpha-l-rhamnopyranosyl)-O-beta-d-glucopyranoside], whereas A2 was cyanidin 3-rutinoside, which is widely distributed in higher plants. Oxygen radical absorbance capacity (ORAC) assays proved their high antioxidant activities. PMID:18406435

  2. Study of Asparagus Radiopreservation (Asparagus Officinalis)

    International Nuclear Information System (INIS)

    The main purpose of this work were find the suitable dose to extend the useful life of this vegetable (asparagus). In addition, physical and chemical changes that could be induced were studied the bio burden of this vegetable. The asparagus sorted, cleaned and cut, was irradiated in Gamma cell 220; the analyzed dose were 0.5, 1.0, 1.5, 2.0 and 4.18 KGy. According to these date, and increased irradiation dose cause that pH and titrated acidity grow rapidly day by day, because of glucose, fructose; exist since small presence of volatile acid or the glucose formed from glucose an fructose exist moisture loss produce weight loss in the asparagus. Dose that gave better results were: 1.5, 2.0 and 0.5 KGy; moisture loss produce acids concentration and des favourable appearance observed in organoleptic analysis. The fibres formed were least in all cases to irradiated asparagus, especially for 1.5 and 2.0 KGy, there were not fat and protein content changes (in that case only there was a test to control and 2.0 KGy). The percent weigh loss and percent size diminution, were less when store conditions were the best (90-95% H.R. and 1-2 oC), the maximum loss was 14.88% to the control and 11.06% to the irradiated product (1.5 KGy). Ascorbic acid content of control was 57.0%, in irradiated products were 21.71% and 37.68% for 2.0 and 1.5 KGy, after storing the products 30 days approximately. Because asparagus bio burden vary in a range from 105 to 106, this is reduce by cleaning up to 103, the irradiation let us reduce in one order. Let's conclude that the best dose to asparagus conservation was 1.5 KGy, the useful life time was 32 days, in store conditions of 90-95 H>R. and temperature 1-2 oC. The irradiation let inhibit microorganisms action until a 15 days period, after this, there was tendency to growth. The least moisture loss and fibre formation were favoured. (author). 28 refs., 33 tabs., 27 ills

  3. Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.)

    OpenAIRE

    Leonardo Galli; Judith Viégas; Eliane Augustin; Marcia Ines Eckert; João Baptista da Silva

    1998-01-01

    Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L.), in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, st...

  4. Meiosis of anther culture regenerants in asparagus (Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Leonardo Galli

    1998-03-01

    Full Text Available Pollen mother cells obtained from regenerated plants of asparagus (Asparagus officinalis L., in a population composed exclusively of male plants, through the process of anther culture from the hybrid G27 X 22-8, were analyzed during meiosis. It was observed that, during theprocess of anther culture by organogenesis, the pollen mother cells of the regenerants had great genomic instability, as evidenced by disturbances in all the meiotic phases of the first and second division. Furthermore, structural chromosomal abnormalities, in addition to aneuploidy and polyploidy, were observed.Foi analisada a meiose em células mãe de pólen de plantas de aspargo (Asparagus officinalis L. de uma população composta exclusivamente de plantas masculinas, obtidas através do processo de cultura de anteras do híbrido G27 X 22-8. Foi observado que, durante o processo de cultura de anteras, via calogênese, as células mãe de pólen dos regenerantes apresentaram grande instabilidade genômica, evidenciada por irregularidades nas fases de diacinese, assim como de metáfase, anáfase, telófase da primeira e segunda divisão meiótica. Além disto, o processo originou anormalidades cromossômicas estruturais em adição às aneuploidias e poliploidias.

  5. Molecular phylogeny of the genus Asparagus (Asparagaceae) explains interspecific crossability between the garden asparagus (A. officinalis) and other Asparagus species.

    Science.gov (United States)

    Kubota, Shosei; Konno, Itaru; Kanno, Akira

    2012-02-01

    The genus Asparagus comprises approximately 200 species, some of which are commercially cultivated, such as the garden asparagus (A. officinalis). Many Asparagus species, including A. officinalis, are dioecious and have been grouped into a subgenus distinct from that of hermaphroditic species. Although many interspecific crossings have been attempted to introduce useful traits into A. officinalis, only some of the dioecious species were found to be cross-compatible with A. officinalis. Here, molecular phylogenetic analyses were conducted to determine whether interspecific crossability is proportional to the genetic distance between the crossing pairs and to further clarify the evolutionary history of the Asparagus genus. A clade with all cross-compatible species and no cross-incompatible species was recovered in the phylogenetic tree based on analyses of non-coding cpDNA regions. In addition, a sex-linked marker developed for A. officinalis amplified a male-specific region in all cross-compatible species. The phylogenetic analyses also provided some insights about the evolutionary history of Asparagus; for example, by indicating that the genus had its origin in southern Africa, subsequently spreading throughout the old world through intensive speciation and dispersal. The results also suggest that dioecious species were derived from a single evolutionary transition from hermaphroditism in Asparagus. These findings not only contribute towards the understanding of the evolutionary history of the genus but may also facilitate future interspecific hybridization programs involving Asparagus species.

  6. Molecular phylogeny of the genus Asparagus (Asparagaceae) explains interspecific crossability between the garden asparagus (A. officinalis) and other Asparagus species.

    Science.gov (United States)

    Kubota, Shosei; Konno, Itaru; Kanno, Akira

    2012-02-01

    The genus Asparagus comprises approximately 200 species, some of which are commercially cultivated, such as the garden asparagus (A. officinalis). Many Asparagus species, including A. officinalis, are dioecious and have been grouped into a subgenus distinct from that of hermaphroditic species. Although many interspecific crossings have been attempted to introduce useful traits into A. officinalis, only some of the dioecious species were found to be cross-compatible with A. officinalis. Here, molecular phylogenetic analyses were conducted to determine whether interspecific crossability is proportional to the genetic distance between the crossing pairs and to further clarify the evolutionary history of the Asparagus genus. A clade with all cross-compatible species and no cross-incompatible species was recovered in the phylogenetic tree based on analyses of non-coding cpDNA regions. In addition, a sex-linked marker developed for A. officinalis amplified a male-specific region in all cross-compatible species. The phylogenetic analyses also provided some insights about the evolutionary history of Asparagus; for example, by indicating that the genus had its origin in southern Africa, subsequently spreading throughout the old world through intensive speciation and dispersal. The results also suggest that dioecious species were derived from a single evolutionary transition from hermaphroditism in Asparagus. These findings not only contribute towards the understanding of the evolutionary history of the genus but may also facilitate future interspecific hybridization programs involving Asparagus species. PMID:21947345

  7. Two new acetylenic compounds from Asparagus officinalis.

    Science.gov (United States)

    Li, Xue-Mei; Cai, Jin-Long; Wang, Wen-Xiang; Ai, Hong-Lian; Mao, Zi-Chao

    2016-01-01

    Two new acetylenic compounds, asparoffins A (1) and B (2), together with two known compounds, nyasol (3) and 3″-methoxynyasol (4), were isolated from stems of Asparagus officinalis. The structures of two new compounds were elucidated on the basis of detailed spectroscopic analyses (UV, IR, MS, 1D, and 2D NMR). All compounds were evaluated for their cytotoxicities against three human cancer cell lines.

  8. Two new acetylenic compounds from Asparagus officinalis.

    Science.gov (United States)

    Li, Xue-Mei; Cai, Jin-Long; Wang, Wen-Xiang; Ai, Hong-Lian; Mao, Zi-Chao

    2016-01-01

    Two new acetylenic compounds, asparoffins A (1) and B (2), together with two known compounds, nyasol (3) and 3″-methoxynyasol (4), were isolated from stems of Asparagus officinalis. The structures of two new compounds were elucidated on the basis of detailed spectroscopic analyses (UV, IR, MS, 1D, and 2D NMR). All compounds were evaluated for their cytotoxicities against three human cancer cell lines. PMID:26558641

  9. Identification of Sensory-Active Phytochemicals in Asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2012-12-01

    Sensory-directed fractionation of extracts prepared from raw and cooked asparagus (Asparagus officinalis L.), respectively, followed by LC-TOF-MS, LC-MS/MS, and 1D/2D-NMR experiments revealed the chemical structures of nine bitter tasting mono- and bidesmotic saponins as well as the previously not reported 1,2-dithiolan-4-carboxylic acid 6-D-α/β-glucopyranose ester exhibiting an interesting buttery mouth-coating effect. Sensory studies showed that the orosensation imparted by this sulfur compound was reminiscent to that of melting butter and revealed an orosensory recognition threshold of 276.8 μmol/L.

  10. Identification of Sensory-Active Phytochemicals in Asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2012-12-01

    Sensory-directed fractionation of extracts prepared from raw and cooked asparagus (Asparagus officinalis L.), respectively, followed by LC-TOF-MS, LC-MS/MS, and 1D/2D-NMR experiments revealed the chemical structures of nine bitter tasting mono- and bidesmotic saponins as well as the previously not reported 1,2-dithiolan-4-carboxylic acid 6-D-α/β-glucopyranose ester exhibiting an interesting buttery mouth-coating effect. Sensory studies showed that the orosensation imparted by this sulfur compound was reminiscent to that of melting butter and revealed an orosensory recognition threshold of 276.8 μmol/L. PMID:23136992

  11. Steroidal saponins from roots of Asparagus officinalis.

    Science.gov (United States)

    Huang, Xuefeng; Kong, Lingyi

    2006-02-01

    Sarsasapogenin M (1) and sarsasapogenin N (2), two new oligospirostanosides with a unique aglycone moiety, (25S)-5beta-spirostan-3beta, 17alpha-diol, along with seven known compounds (25S)-5beta-spirostan-3beta-ol-3-O-beta-d-glucopyranosyl-(1,2)-[beta-d-xylopyranosyl-(1,4)]-beta-d-glucopyranoside (3), (25S)-5beta-spirostan-3beta-ol-3-O-beta-d-glucopyranosyl-(1,2)-beta-d-glucopyranoside (4), (25S)-5beta-spirostan-3beta-ol-3-O-alpha-l-rhamnopyranosyl-(1,2)-[alpha-l-rhamnopyranosyl-(1,4)]-beta-d-glucopyranoside (5), (25S)26-O-beta-d-glucopyranosyl-5beta-furost-20 (22)-ene-3beta,26-diol-3-O-beta-d-glucopyranosyl-(1,2)-beta-d-glucopyranoside (6), yamogenin (7), beta-sitosterol (8), and sitosterol-beta-d-glucoside (9) were isolated from the roots of Asparagus officinalis L. Their structures were determined by spectral analysis, including extensive 1D and 2D NMR experiments. PMID:16280142

  12. Occurrence and characterization of a Phytophthora sp. pathogenic to asparagus (Asparagus officinalis) in Michigan.

    Science.gov (United States)

    Saude, C; Hurtado-Gonzales, O P; Lamour, K H; Hausbeck, M K

    2008-10-01

    A homothallic Phytophthora sp. was recovered from asparagus (Asparagus officinalis) spears, storage roots, crowns, and stems in northwest and central Michigan in 2004 and 2005. Isolates (n = 131) produced ovoid, nonpapillate, noncaducous sporangia 45 microm long x 26 microm wide and amphigynous oospores of 25 to 30 microm diameter. Mycelial growth was optimum at 25 degrees C with no growth at 5 and 30 degrees C. All isolates were sensitive to 100 ppm mefenoxam. Pathogenicity studies confirmed the ability of the isolates to infect asparagus as well as cucurbits. Amplified fragment length polymorphism analysis of 99 isolates revealed identical fingerprints, with 12 clearly resolved fragments present and no clearly resolved polymorphic fragments, suggesting a single clonal lineage. The internal transcribed spacer regions of representative isolates were homologous with a Phytophthora sp. isolated from diseased asparagus in France and a Phytophthora sp. from agave in Australia. Phylogenetic analysis supports the conclusion that the Phytophthora sp. isolated from asparagus in Michigan is a distinct species, and has been named Phytophthora asparagi.

  13. Occurrence and characterization of a Phytophthora sp. pathogenic to asparagus (Asparagus officinalis) in Michigan.

    Science.gov (United States)

    Saude, C; Hurtado-Gonzales, O P; Lamour, K H; Hausbeck, M K

    2008-10-01

    A homothallic Phytophthora sp. was recovered from asparagus (Asparagus officinalis) spears, storage roots, crowns, and stems in northwest and central Michigan in 2004 and 2005. Isolates (n = 131) produced ovoid, nonpapillate, noncaducous sporangia 45 microm long x 26 microm wide and amphigynous oospores of 25 to 30 microm diameter. Mycelial growth was optimum at 25 degrees C with no growth at 5 and 30 degrees C. All isolates were sensitive to 100 ppm mefenoxam. Pathogenicity studies confirmed the ability of the isolates to infect asparagus as well as cucurbits. Amplified fragment length polymorphism analysis of 99 isolates revealed identical fingerprints, with 12 clearly resolved fragments present and no clearly resolved polymorphic fragments, suggesting a single clonal lineage. The internal transcribed spacer regions of representative isolates were homologous with a Phytophthora sp. isolated from diseased asparagus in France and a Phytophthora sp. from agave in Australia. Phylogenetic analysis supports the conclusion that the Phytophthora sp. isolated from asparagus in Michigan is a distinct species, and has been named Phytophthora asparagi. PMID:18943453

  14. Wildly Growing Asparagus (Asparagus officinalis L.) Hosts Pathogenic Fusarium Species and Accumulates Their Mycotoxins.

    Science.gov (United States)

    Stępień, Łukasz; Waśkiewicz, Agnieszka; Urbaniak, Monika

    2016-05-01

    Asparagus officinalis L. is an important crop in many European countries, likely infected by a number of Fusarium species. Most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. However, there is lack of information on Fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. Therefore, the main scientific aim of this study was to identify the Fusarium species and quantify their typical mycotoxins present in wild asparagus plants collected at four time points of the season. Forty-four Fusarium strains of eight species--Fusarium acuminatum, Fusarium avenaceum, Fusarium culmorum, Fusarium equiseti, Fusarium oxysporum, Fusarium proliferatum, Fusarium sporotrichioides, and Fusarium tricinctum--were isolated from nine wild asparagus plants in 2013 season. It is the first report of F. sporotrichioides isolated from this particular host. Fumonisin B1 was the most abundant mycotoxin, and the highest concentrations of fumonisins B1-B3 and beauvericin were found in the spears collected in May. Moniliformin and enniatins were quantified at lower concentrations. Mycotoxins synthesized by individual strains obtained from infected asparagus tissues were assessed using in vitro cultures on sterile rice grain. Most of the F. sporotrichioides strains synthesized HT-2 toxin and F. equiseti strains were found to be effective zearalenone producers. PMID:26687343

  15. Life table and consumption capacity of corn earworm, Helicoverpa armigera, fed asparagus, Asparagus officinalis.

    Science.gov (United States)

    Jha, Ratna Kumar; Tuan, Shu-Jen; Chi, Hsin; Tang, Li-Cheng

    2014-03-01

    The life table and consumption rate of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) reared on asparagus, Asparagus officinalis L. (Asparagales: Asparagaceae) were studied under laboratory conditions to assess their interaction. Development, survival, fecundity, and consumption data were analyzed by the age-stage, twosex life table. This study indicated that asparagus is a natural host of H. armigera. However, the poor nutritional content in asparagus foliage and the poor fitness of H. armigera that fed on asparagus indicated that asparagus is a suboptimal host in comparison to hybrid sweet corn. The uncertainty associated with life table parameters was estimated by using jackknife and bootstrap techniques, and the results were compared for statistical inference. The intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), and mean generation time (T) were estimated by the jackknife technique to be 0.0780 day(-1), 1.0811 day(-1), 67.4 offspring, and 54.8 days, respectively, while those estimated by the bootstrap technique were 0.0752 day(-1), 1.0781 day(-1), 68.0 offspring, and 55.3 days, respectively. The net consumption rate of H. armigera, as estimated by the jackknife and bootstrap technique, was 1183.02 and 1132.9 mg per individual, respectively. The frequency distribution of sample means obtained by the jackknife technique failed the normality test, while the bootstrap results fit the normal distribution well. By contrast, the relationship between the mean fecundity and the net reproductive rate, as estimated by the bootstrap technique, was slightly inconsistent with the relationship found by mathematical proof. The application of the jackknife and bootstrap techniques in estimating population parameters requires further examination.

  16. Life table and consumption capacity of corn earworm, Helicoverpa armigera, fed asparagus, Asparagus officinalis.

    Science.gov (United States)

    Jha, Ratna Kumar; Tuan, Shu-Jen; Chi, Hsin; Tang, Li-Cheng

    2014-01-01

    The life table and consumption rate of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) reared on asparagus, Asparagus officinalis L. (Asparagales: Asparagaceae) were studied under laboratory conditions to assess their interaction. Development, survival, fecundity, and consumption data were analyzed by the age-stage, twosex life table. This study indicated that asparagus is a natural host of H. armigera. However, the poor nutritional content in asparagus foliage and the poor fitness of H. armigera that fed on asparagus indicated that asparagus is a suboptimal host in comparison to hybrid sweet corn. The uncertainty associated with life table parameters was estimated by using jackknife and bootstrap techniques, and the results were compared for statistical inference. The intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), and mean generation time (T) were estimated by the jackknife technique to be 0.0780 day(-1), 1.0811 day(-1), 67.4 offspring, and 54.8 days, respectively, while those estimated by the bootstrap technique were 0.0752 day(-1), 1.0781 day(-1), 68.0 offspring, and 55.3 days, respectively. The net consumption rate of H. armigera, as estimated by the jackknife and bootstrap technique, was 1183.02 and 1132.9 mg per individual, respectively. The frequency distribution of sample means obtained by the jackknife technique failed the normality test, while the bootstrap results fit the normal distribution well. By contrast, the relationship between the mean fecundity and the net reproductive rate, as estimated by the bootstrap technique, was slightly inconsistent with the relationship found by mathematical proof. The application of the jackknife and bootstrap techniques in estimating population parameters requires further examination. PMID:25373181

  17. Quantitation and bitter taste contribution of saponins in fresh and cooked white asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2014-02-15

    A sensitive HPLC-MS/MS method was developed enabling the simultaneous quantification of bitter-tasting mono- and bidesmosidic saponins in fresh and processed asparagus (Asparagus officinalis L.). Based on quantitative data and bitter taste recognition thresholds, dose-over-threshold factors were determined for the first time to determine the bitter impact of the individual saponins. Although 3-O-[α-L-rhamnopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl]-(25R/S)-spirost-5-ene-3β-ol was found based on dose-over-threshold factors to be the predominant bitter saponin in raw asparagus spears, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-{α-L-rhamnopyranosyl-(1 → 4)}-β-D-glucopyranosyl]-26-O-[β-D-glucopyranosyl]-(25R)-22-hydroxyfurost-5-ene-3β,26-diol, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-{α-L-rhamnopyranosyl-(1 → 4)}-β-D-glucopyranosyl]-26-O-[β-D-glucopyranosyl]-(25S)-22-hydroxyfurost-5-ene-3β,26-diol, and (25R)- and (25S)-furost-5-en-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside were found as key bitter contributors after cooking. Interestingly, the monodesmosidic saponins 5a/b were demonstrated for the first time to be the major contributor to the bitter taste of fresh asparagus spears, while the bidesmosides 1a/b and 2a/b may be considered the primary determinants for the bitter taste of cooked asparagus.

  18. Quantitation and bitter taste contribution of saponins in fresh and cooked white asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2014-02-15

    A sensitive HPLC-MS/MS method was developed enabling the simultaneous quantification of bitter-tasting mono- and bidesmosidic saponins in fresh and processed asparagus (Asparagus officinalis L.). Based on quantitative data and bitter taste recognition thresholds, dose-over-threshold factors were determined for the first time to determine the bitter impact of the individual saponins. Although 3-O-[α-L-rhamnopyranosyl-(1→2)-α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranosyl]-(25R/S)-spirost-5-ene-3β-ol was found based on dose-over-threshold factors to be the predominant bitter saponin in raw asparagus spears, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-{α-L-rhamnopyranosyl-(1 → 4)}-β-D-glucopyranosyl]-26-O-[β-D-glucopyranosyl]-(25R)-22-hydroxyfurost-5-ene-3β,26-diol, 3-O-[α-L-rhamnopyranosyl-(1 → 2)-{α-L-rhamnopyranosyl-(1 → 4)}-β-D-glucopyranosyl]-26-O-[β-D-glucopyranosyl]-(25S)-22-hydroxyfurost-5-ene-3β,26-diol, and (25R)- and (25S)-furost-5-en-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1 → 4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside were found as key bitter contributors after cooking. Interestingly, the monodesmosidic saponins 5a/b were demonstrated for the first time to be the major contributor to the bitter taste of fresh asparagus spears, while the bidesmosides 1a/b and 2a/b may be considered the primary determinants for the bitter taste of cooked asparagus. PMID:24128498

  19. Structural and Sensory Characterization of Bitter Tasting Steroidal Saponins from Asparagus Spears (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2012-12-01

    Application of sequential solvent extraction and iterative chromatographic separation in combination with taste dilution analysis recently revealed a series of steroidal saponins as the key contributors to the typical bitter taste of white asparagus spears (Asparagus officinalis L.). Besides six previously reported saponins, (25R)-furost-5-en-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, (25R)-furostane-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, and (25S)-furostane-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, and 3-O-[{α-L-rhamnopyranosyl-(1→2)}{α-L-rhamnopyranosyl-(1→4)}-β-D-glucopyranosyl]-(25S)-spirost-5-ene-3β-ol were identified for the first time as key bitter compounds in the edible spears of white asparagus by means of LC-MS/MS, LC-TOF-MS, 1D/2D-NMR spectroscopy, and hydrolysis experiments. This paper presents the isolation, structure determination, and sensory activity of these saponins. Depending on their chemical structure, the saponins identified showed human bitter recognition thresholds between 10.9 and 199.7 μmol/L (water).

  20. Sex-biased gene expression in dioecious garden asparagus (Asparagus officinalis).

    Science.gov (United States)

    Harkess, Alex; Mercati, Francesco; Shan, Hong-Yan; Sunseri, Francesco; Falavigna, Agostino; Leebens-Mack, Jim

    2015-08-01

    Sex chromosomes have evolved independently in phylogenetically diverse flowering plant lineages. The genes governing sex determination in dioecious species remain unknown, but theory predicts that the linkage of genes influencing male and female function will spur the origin and early evolution of sex chromosomes. For example, in an XY system, the origin of an active Y may be spurred by the linkage of female suppressing and male promoting genes. Garden asparagus (Asparagus officinalis) serves as a model for plant sex chromosome evolution, given that it has recently evolved an XX/XY sex chromosome system. In order to elucidate the molecular basis of gender differences and sex determination, we used RNA-sequencing (RNA-Seq) to identify differentially expressed genes between female (XX), male (XY) and supermale (YY) individuals. We identified 570 differentially expressed genes, and showed that significantly more genes exhibited male-biased than female-biased expression in garden asparagus. In the context of anther development, we identified genes involved in pollen microspore and tapetum development that were specifically expressed in males and supermales. Comparative analysis of genes in the Arabidopsis thaliana, Zea mays and Oryza sativa anther development pathways shows that anther sterility in females probably occurs through interruption of tapetum development before microspore meiosis.

  1. Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Mercati, Francesco; Riccardi, Paolo; Leebens-Mack, Jim; Abenavoli, Maria Rosa; Falavigna, Agostino; Sunseri, Francesco

    2013-04-01

    Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSR) are abundant and evenly distributed co-dominant molecular markers in plant genomes. SSRs are valuable for marker assisted breeding and positional cloning of genes associated traits of interest. Although several high throughput platforms have been developed to identify SNP and SSR markers for analysis of segregant plant populations, breeding in garden asparagus (Asparagus officinalis L.) has been limited by a low content of such markers. In this study massively parallel GS-FLX pyro-sequencing technology (454 Life Sciences) has been used to sequence and compare transcriptome from two genotypes: a rust tolerant male (1770) and a susceptible female (G190). A total of 122,963 and 99,368 sequence reads, with an average length of 245.7bp, have been recovered from accessions 1770 and 190 respectively. A computational pipeline has been used to predict and visually inspect putative SNPs and SSR sequences. Analysis of Gene Ontology (GO) slim annotation assignments for all assembled uniscripts indicated that the 24,403 assemblies represent genes from a broad array of functions. Further, over 1800 putative SNPs and 1000 SSRs were detected. One hundred forty-four SNPs together with 60 selected SSRs were validated and used to develop a preliminary genetic map by using a large BC(1) population, derived from 1770 and G190. The abundance of SNPs and SSRs provides a foundation for the development of saturated genetic maps and their utilization in assisted asparagus breeding programs.

  2. Sex-biased gene expression in dioecious garden asparagus (Asparagus officinalis).

    Science.gov (United States)

    Harkess, Alex; Mercati, Francesco; Shan, Hong-Yan; Sunseri, Francesco; Falavigna, Agostino; Leebens-Mack, Jim

    2015-08-01

    Sex chromosomes have evolved independently in phylogenetically diverse flowering plant lineages. The genes governing sex determination in dioecious species remain unknown, but theory predicts that the linkage of genes influencing male and female function will spur the origin and early evolution of sex chromosomes. For example, in an XY system, the origin of an active Y may be spurred by the linkage of female suppressing and male promoting genes. Garden asparagus (Asparagus officinalis) serves as a model for plant sex chromosome evolution, given that it has recently evolved an XX/XY sex chromosome system. In order to elucidate the molecular basis of gender differences and sex determination, we used RNA-sequencing (RNA-Seq) to identify differentially expressed genes between female (XX), male (XY) and supermale (YY) individuals. We identified 570 differentially expressed genes, and showed that significantly more genes exhibited male-biased than female-biased expression in garden asparagus. In the context of anther development, we identified genes involved in pollen microspore and tapetum development that were specifically expressed in males and supermales. Comparative analysis of genes in the Arabidopsis thaliana, Zea mays and Oryza sativa anther development pathways shows that anther sterility in females probably occurs through interruption of tapetum development before microspore meiosis. PMID:25817071

  3. Structural and Sensory Characterization of Bitter Tasting Steroidal Saponins from Asparagus Spears (Asparagus officinalis L.).

    Science.gov (United States)

    Dawid, Corinna; Hofmann, Thomas

    2012-12-01

    Application of sequential solvent extraction and iterative chromatographic separation in combination with taste dilution analysis recently revealed a series of steroidal saponins as the key contributors to the typical bitter taste of white asparagus spears (Asparagus officinalis L.). Besides six previously reported saponins, (25R)-furost-5-en-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, (25R)-furostane-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, and (25S)-furostane-3β,22,26-triol-3-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside]-26-O-β-D-glucopyranoside, and 3-O-[{α-L-rhamnopyranosyl-(1→2)}{α-L-rhamnopyranosyl-(1→4)}-β-D-glucopyranosyl]-(25S)-spirost-5-ene-3β-ol were identified for the first time as key bitter compounds in the edible spears of white asparagus by means of LC-MS/MS, LC-TOF-MS, 1D/2D-NMR spectroscopy, and hydrolysis experiments. This paper presents the isolation, structure determination, and sensory activity of these saponins. Depending on their chemical structure, the saponins identified showed human bitter recognition thresholds between 10.9 and 199.7 μmol/L (water). PMID:23137023

  4. Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.).

    Science.gov (United States)

    Mercati, Francesco; Riccardi, Paolo; Leebens-Mack, Jim; Abenavoli, Maria Rosa; Falavigna, Agostino; Sunseri, Francesco

    2013-04-01

    Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSR) are abundant and evenly distributed co-dominant molecular markers in plant genomes. SSRs are valuable for marker assisted breeding and positional cloning of genes associated traits of interest. Although several high throughput platforms have been developed to identify SNP and SSR markers for analysis of segregant plant populations, breeding in garden asparagus (Asparagus officinalis L.) has been limited by a low content of such markers. In this study massively parallel GS-FLX pyro-sequencing technology (454 Life Sciences) has been used to sequence and compare transcriptome from two genotypes: a rust tolerant male (1770) and a susceptible female (G190). A total of 122,963 and 99,368 sequence reads, with an average length of 245.7bp, have been recovered from accessions 1770 and 190 respectively. A computational pipeline has been used to predict and visually inspect putative SNPs and SSR sequences. Analysis of Gene Ontology (GO) slim annotation assignments for all assembled uniscripts indicated that the 24,403 assemblies represent genes from a broad array of functions. Further, over 1800 putative SNPs and 1000 SSRs were detected. One hundred forty-four SNPs together with 60 selected SSRs were validated and used to develop a preliminary genetic map by using a large BC(1) population, derived from 1770 and G190. The abundance of SNPs and SSRs provides a foundation for the development of saturated genetic maps and their utilization in assisted asparagus breeding programs. PMID:23415335

  5. Viruses of asparagus.

    Science.gov (United States)

    Tomassoli, Laura; Tiberini, Antonio; Vetten, Heinrich-Josef

    2012-01-01

    The current knowledge on viruses infecting asparagus (Asparagus officinalis) is reviewed. Over half a century, nine virus species belonging to the genera Ilarvirus, Cucumovirus, Nepovirus, Tobamovirus, Potexvirus, and Potyvirus have been found in this crop. The potyvirus Asparagus virus 1 (AV1) and the ilarvirus Asparagus virus 2 (AV2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. The main properties and epidemiology of AV1 and AV2 as well as diagnostic techniques for their detection and identification are described. Minor viruses and control are briefly outlined.

  6. Viruses of asparagus.

    Science.gov (United States)

    Tomassoli, Laura; Tiberini, Antonio; Vetten, Heinrich-Josef

    2012-01-01

    The current knowledge on viruses infecting asparagus (Asparagus officinalis) is reviewed. Over half a century, nine virus species belonging to the genera Ilarvirus, Cucumovirus, Nepovirus, Tobamovirus, Potexvirus, and Potyvirus have been found in this crop. The potyvirus Asparagus virus 1 (AV1) and the ilarvirus Asparagus virus 2 (AV2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. The main properties and epidemiology of AV1 and AV2 as well as diagnostic techniques for their detection and identification are described. Minor viruses and control are briefly outlined. PMID:22682173

  7. Evaluación de siete poblaciones de espárrago (Asparagus officinalis L. Evaluation of seven asparagus populations (Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    ILEANA GATTI

    2000-06-01

    Full Text Available Los materiales de espárrago (Asparagus officinalis L. utilizados hasta el momento por los productores son introducciones realizadas por las casas semilleras y han sido seleccionados para satisfacer requerimientos del mercado de los sitios de origen. El objetivo del siguiente trabajo es evaluar siete poblaciones de espárrago (P1 a P7, con el fin de seleccionar genitores adecuados de manera de recurrir a la hibridación de ellos para obtener materiales adaptados a los requerimientos locales. Las evaluaciones se hicieron sobre plantas individuales, separadas por sexos y manejadas como espárrago blanco, durante los años 1993 y 1994, en el campo experimental de la Facultad de Ciencias Agrarias (Universidad Nacional de Rosario, ubicado en Zavalla, provincia de Santa Fe. La evaluación se realizó sobre planta individual, durante un período de 40 días de cosecha y con los datos obtenidos se realizó un ANOVA y un análisis de agrupamiento. Para elegir progenitores femeninos con altos rendimientos y rendimiento de mercado se determinó que se deberá recurrir a las P1, P2 y P3, las cuales presentan también alto número de turiones. Para altos peso medio y diámetro de turión, así como producción tardía, son indicadas las P5 y P7 como genitores masculinos, teniendo en cuenta que, mientras la P7 presenta bajo rendimiento, la P5 aportaría mejores producciones.The materials of asparagus (Asparagus officinalis L. that growers have in use are imported by seed dealers and have been selected according to overseas markets requirements. With the aim of selecting proper parents to use in a breeding program, seven populations of Asparagus officinalis (P1 to P7, all rised as white asparagus, were tested during 1993 and 1994, at the Experimental Field of the Facultad de Ciencias Agrarias (Universidad Nacional de Rosario placed in Zavalla, Santa Fe, Argentina. The evaluation was made on individual plant in a 40 days of harvest period. ANOVA and a cluster

  8. Egg attachment of the asparagus beetle Crioceris asparagi to the crystalline waxy surface of Asparagus officinalis.

    Science.gov (United States)

    Voigt, Dagmar; Gorb, Stanislav

    2010-03-22

    Plant surfaces covered with crystalline epicuticular waxes are known to be anti-adhesive, hardly wettable and preventing insect attachment. But there are insects that are capable of gluing their eggs to these surfaces by means of proteinaceous secretions. In this study, we analysed the bonding region between the eggs of Crioceris asparagi and the plant surface of Asparagus officinalis using light and cryo-scanning electron microscopy. The wettability of the plant surface by egg secretion was compared with that by Aqua Millipore water, aqueous sugar solution and chicken egg white. Furthermore, the force required to remove C. asparagi eggs from the plant surface was measured, in order to evaluate the egg's bonding strength. Mean pull-off force was 14.7 mN, which is about 8650 times higher than the egg weight. Egg glue was observed spreading over the wax crystal arrays on the plant cladophyll and wetting them. Similar wetting behaviour on the A. officinalis surface was observed for chicken egg white. Our results support the hypothesis that the mechanism of insect egg adhesion on micro- and nanostructured hydrophobic plant surfaces is related to the proteinaceous nature of adhesive secretions of insect eggs. The secretion wets superhydrophobic surfaces and after solidifying builds up a composite, consisting of the solidified glue and wax crystals, at the interface between the egg and plant cuticle.

  9. Egg attachment of the asparagus beetle Crioceris asparagi to the crystalline waxy surface of Asparagus officinalis.

    Science.gov (United States)

    Voigt, Dagmar; Gorb, Stanislav

    2010-03-22

    Plant surfaces covered with crystalline epicuticular waxes are known to be anti-adhesive, hardly wettable and preventing insect attachment. But there are insects that are capable of gluing their eggs to these surfaces by means of proteinaceous secretions. In this study, we analysed the bonding region between the eggs of Crioceris asparagi and the plant surface of Asparagus officinalis using light and cryo-scanning electron microscopy. The wettability of the plant surface by egg secretion was compared with that by Aqua Millipore water, aqueous sugar solution and chicken egg white. Furthermore, the force required to remove C. asparagi eggs from the plant surface was measured, in order to evaluate the egg's bonding strength. Mean pull-off force was 14.7 mN, which is about 8650 times higher than the egg weight. Egg glue was observed spreading over the wax crystal arrays on the plant cladophyll and wetting them. Similar wetting behaviour on the A. officinalis surface was observed for chicken egg white. Our results support the hypothesis that the mechanism of insect egg adhesion on micro- and nanostructured hydrophobic plant surfaces is related to the proteinaceous nature of adhesive secretions of insect eggs. The secretion wets superhydrophobic surfaces and after solidifying builds up a composite, consisting of the solidified glue and wax crystals, at the interface between the egg and plant cuticle. PMID:19923132

  10. Asparagus Beetle and Spotted Asparagus Beetle

    OpenAIRE

    Erin W Hodgson; Drost, Dan

    2007-01-01

    Asparagus beetle, Crioceris asparagi, and spotted asparagus beetle, C. duodecimpunctata are leaf beetles in the family Chrysomelidae. These beetles feed exclusively on asparagus and are native to Europe. Asparagus beetle is the more economically injurious of the two species.

  11. Effect of the gamma radiation in the metabolic activity of the apical meristem of asparagus (Asparagus officinalis)

    International Nuclear Information System (INIS)

    The asparagus (Asparagus officinalis) is an agricultural product whose production generates a great quantity of wages as well as foreign currencies for the country because a great part of its production is exported to international markets. In direct reason to the high metabolic activity of the apical meristem, this product it possesses a short shelf life under good conditions of commercialization. Due to the above mentioned, the present work had as objective to evaluate the effect of the gamma radiation in the metabolic activity of the apical meristem of turions of asparagus during the postharvest. Turions of asparagus variety Brock of standard quality was treated with gamma radiation to absorbed dose of 1.0, 1.5, 2.0, 2.5 and 3.0 kGy using an irradiator of 60Co and stored in one controlled temperature camera maintained at 2 C during 8 days. During the experiment, the camera stayed in darkness and under conditions of high relative humidity by means of the water aspersion. Immediately before the one treatment and after 1, 2, 4 and 8 days of storage, the asparagus were sampled to evaluate the breathing speed (VRCG) by means of gas chromatography and scanning differential calorimetry (VRCDB), ethylene production (PE) by means of gas chromatography and production of metabolic heat of the apical meristem of the asparagus by means of scanning differential calorimetry (Q). Its were not found effects due to the gamma radiation in the variables of VRCG, VRCDB and Q. However, for the PE case, it was found that the doses of 1.5 and 2.5 reduced the PE from the first day of storage, while the 3 kGy dose achievement to eliminate completely the ethylene production from the first day of storage. It was concluded that the gamma radiation at the used levels in the present experiment doesn't reduce the metabolic activity of the apical meristem of the turion of asparagus although it can to improve the postharvest quality from the asparagus when reducing the ethylene production

  12. Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells.

    Science.gov (United States)

    Zhong, Chunge; Jiang, Chunyu; Xia, Xichun; Mu, Teng; Wei, Lige; Lou, Yuntian; Zhang, Xiaoshu; Zhao, Yuqing; Bi, Xiuli

    2015-07-01

    Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC50 value of 45.52 μg/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-α. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-β1, activin C) and accumulation of TNF-α, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-β1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade.

  13. Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells.

    Science.gov (United States)

    Zhong, Chunge; Jiang, Chunyu; Xia, Xichun; Mu, Teng; Wei, Lige; Lou, Yuntian; Zhang, Xiaoshu; Zhao, Yuqing; Bi, Xiuli

    2015-07-01

    Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC50 value of 45.52 μg/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-α. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-β1, activin C) and accumulation of TNF-α, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-β1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade. PMID:26089141

  14. Effects of hydrogen peroxide treatment on thiol contents in fresh-cut asparagus (Asparagus officinalis) spears.

    Science.gov (United States)

    Demrkol, Omca

    2009-01-01

    In this work, the impact of hydrogen peroxide (H2O2) was investigated on the thiol content of asparagus. Fresh-cut asparagus was treated with H2O2 at varied oxidant concentrations and contact times. A significant decrease (alpha=0.05) was observed in N-acetylcysteine levels treated with 2.5% H2O2 for 10 min and with 5% H2O2 for 3, 5 and 10 min. Captopril and cysteine levels significantly decreased (alpha=0.05) in all and most treatment conditions, respectively. Glutathione levels only significantly decreased with 2.5% and 5% H2O2 for 10 min treatment. In order to determine whether asparagus undergoes oxidative stress, a well-known oxidative stress indicator-the glutathione/oxidized glutathione ratio-was calculated. This study showed that the common use of H2O2 as a disinfectant/sterilizer by the food industry could markedly diminish the important biothiols and develop oxidative stress in asparagus, and potentially in other vegetables as well.

  15. Effects of hydrogen peroxide treatment on thiol contents in fresh-cut asparagus (Asparagus officinalis) spears.

    Science.gov (United States)

    Demrkol, Omca

    2009-01-01

    In this work, the impact of hydrogen peroxide (H2O2) was investigated on the thiol content of asparagus. Fresh-cut asparagus was treated with H2O2 at varied oxidant concentrations and contact times. A significant decrease (alpha=0.05) was observed in N-acetylcysteine levels treated with 2.5% H2O2 for 10 min and with 5% H2O2 for 3, 5 and 10 min. Captopril and cysteine levels significantly decreased (alpha=0.05) in all and most treatment conditions, respectively. Glutathione levels only significantly decreased with 2.5% and 5% H2O2 for 10 min treatment. In order to determine whether asparagus undergoes oxidative stress, a well-known oxidative stress indicator-the glutathione/oxidized glutathione ratio-was calculated. This study showed that the common use of H2O2 as a disinfectant/sterilizer by the food industry could markedly diminish the important biothiols and develop oxidative stress in asparagus, and potentially in other vegetables as well. PMID:18608548

  16. Green asparagus (Asparagus officinalis) prevented hypertension by an inhibitory effect on angiotensin-converting enzyme activity in the kidney of spontaneously hypertensive rats.

    Science.gov (United States)

    Sanae, Matsuda; Yasuo, Aoyagi

    2013-06-12

    Green asparagus (Asparagus officinalis) is known to be rich in functional components. In the present study, spontaneously hypertensive rats (SHR) were used to clarify whether green asparagus prevents hypertension by inhibition of angiotensin-converting enzyme (ACE) activity. Six-week-old male SHR were fed a diet with (AD group) or without (ND group) 5% asparagus for 10 weeks. Systolic blood pressure (SBP) (AD: 159 ± 4.8 mmHg, ND: 192 ± 14.7 mmHg), urinary protein excretion/creatinine excretion, and ACE activity in the kidney were significantly lower in the AD group compared with the ND group. Creatinine clearance was significantly higher in the AD group compared with the ND group. In addition, ACE inhibitory activity was observed in a boiling water extract of asparagus. The ACE inhibitor purified and isolated from asparagus was identified as 2″-hydroxynicotianamine. In conclusion, 2″-hydroxynicotianamine in asparagus may be one of the factors inhibiting ACE activity in the kidney, thus preventing hypertension and preserving renal function.

  17. Green asparagus (Asparagus officinalis) prevented hypertension by an inhibitory effect on angiotensin-converting enzyme activity in the kidney of spontaneously hypertensive rats.

    Science.gov (United States)

    Sanae, Matsuda; Yasuo, Aoyagi

    2013-06-12

    Green asparagus (Asparagus officinalis) is known to be rich in functional components. In the present study, spontaneously hypertensive rats (SHR) were used to clarify whether green asparagus prevents hypertension by inhibition of angiotensin-converting enzyme (ACE) activity. Six-week-old male SHR were fed a diet with (AD group) or without (ND group) 5% asparagus for 10 weeks. Systolic blood pressure (SBP) (AD: 159 ± 4.8 mmHg, ND: 192 ± 14.7 mmHg), urinary protein excretion/creatinine excretion, and ACE activity in the kidney were significantly lower in the AD group compared with the ND group. Creatinine clearance was significantly higher in the AD group compared with the ND group. In addition, ACE inhibitory activity was observed in a boiling water extract of asparagus. The ACE inhibitor purified and isolated from asparagus was identified as 2″-hydroxynicotianamine. In conclusion, 2″-hydroxynicotianamine in asparagus may be one of the factors inhibiting ACE activity in the kidney, thus preventing hypertension and preserving renal function. PMID:23647085

  18. Analysis of transposable elements in the genome of Asparagus officinalis from high coverage sequence data.

    Science.gov (United States)

    Li, Shu-Fen; Gao, Wu-Jun; Zhao, Xin-Peng; Dong, Tian-Yu; Deng, Chuan-Liang; Lu, Long-Dou

    2014-01-01

    Asparagus officinalis is an economically and nutritionally important vegetable crop that is widely cultivated and is used as a model dioecious species to study plant sex determination and sex chromosome evolution. To improve our understanding of its genome composition, especially with respect to transposable elements (TEs), which make up the majority of the genome, we performed Illumina HiSeq2000 sequencing of both male and female asparagus genomes followed by bioinformatics analysis. We generated 17 Gb of sequence (12×coverage) and assembled them into 163,406 scaffolds with a total cumulated length of 400 Mbp, which represent about 30% of asparagus genome. Overall, TEs masked about 53% of the A. officinalis assembly. Majority of the identified TEs belonged to LTR retrotransposons, which constitute about 28% of genomic DNA, with Ty1/copia elements being more diverse and accumulated to higher copy numbers than Ty3/gypsy. Compared with LTR retrotransposons, non-LTR retrotransposons and DNA transposons were relatively rare. In addition, comparison of the abundance of the TE groups between male and female genomes showed that the overall TE composition was highly similar, with only slight differences in the abundance of several TE groups, which is consistent with the relatively recent origin of asparagus sex chromosomes. This study greatly improves our knowledge of the repetitive sequence construction of asparagus, which facilitates the identification of TEs responsible for the early evolution of plant sex chromosomes and is helpful for further studies on this dioecious plant.

  19. Analysis of transposable elements in the genome of Asparagus officinalis from high coverage sequence data.

    Directory of Open Access Journals (Sweden)

    Shu-Fen Li

    Full Text Available Asparagus officinalis is an economically and nutritionally important vegetable crop that is widely cultivated and is used as a model dioecious species to study plant sex determination and sex chromosome evolution. To improve our understanding of its genome composition, especially with respect to transposable elements (TEs, which make up the majority of the genome, we performed Illumina HiSeq2000 sequencing of both male and female asparagus genomes followed by bioinformatics analysis. We generated 17 Gb of sequence (12×coverage and assembled them into 163,406 scaffolds with a total cumulated length of 400 Mbp, which represent about 30% of asparagus genome. Overall, TEs masked about 53% of the A. officinalis assembly. Majority of the identified TEs belonged to LTR retrotransposons, which constitute about 28% of genomic DNA, with Ty1/copia elements being more diverse and accumulated to higher copy numbers than Ty3/gypsy. Compared with LTR retrotransposons, non-LTR retrotransposons and DNA transposons were relatively rare. In addition, comparison of the abundance of the TE groups between male and female genomes showed that the overall TE composition was highly similar, with only slight differences in the abundance of several TE groups, which is consistent with the relatively recent origin of asparagus sex chromosomes. This study greatly improves our knowledge of the repetitive sequence construction of asparagus, which facilitates the identification of TEs responsible for the early evolution of plant sex chromosomes and is helpful for further studies on this dioecious plant.

  20. Analysis of transposable elements in the genome of Asparagus officinalis from high coverage sequence data.

    Science.gov (United States)

    Li, Shu-Fen; Gao, Wu-Jun; Zhao, Xin-Peng; Dong, Tian-Yu; Deng, Chuan-Liang; Lu, Long-Dou

    2014-01-01

    Asparagus officinalis is an economically and nutritionally important vegetable crop that is widely cultivated and is used as a model dioecious species to study plant sex determination and sex chromosome evolution. To improve our understanding of its genome composition, especially with respect to transposable elements (TEs), which make up the majority of the genome, we performed Illumina HiSeq2000 sequencing of both male and female asparagus genomes followed by bioinformatics analysis. We generated 17 Gb of sequence (12×coverage) and assembled them into 163,406 scaffolds with a total cumulated length of 400 Mbp, which represent about 30% of asparagus genome. Overall, TEs masked about 53% of the A. officinalis assembly. Majority of the identified TEs belonged to LTR retrotransposons, which constitute about 28% of genomic DNA, with Ty1/copia elements being more diverse and accumulated to higher copy numbers than Ty3/gypsy. Compared with LTR retrotransposons, non-LTR retrotransposons and DNA transposons were relatively rare. In addition, comparison of the abundance of the TE groups between male and female genomes showed that the overall TE composition was highly similar, with only slight differences in the abundance of several TE groups, which is consistent with the relatively recent origin of asparagus sex chromosomes. This study greatly improves our knowledge of the repetitive sequence construction of asparagus, which facilitates the identification of TEs responsible for the early evolution of plant sex chromosomes and is helpful for further studies on this dioecious plant. PMID:24810432

  1. Analysis of Transposable Elements in the Genome of Asparagus officinalis from High Coverage Sequence Data

    Science.gov (United States)

    Li, Shu-Fen; Gao, Wu-Jun; Zhao, Xin-Peng; Dong, Tian-Yu; Deng, Chuan-Liang; Lu, Long-Dou

    2014-01-01

    Asparagus officinalis is an economically and nutritionally important vegetable crop that is widely cultivated and is used as a model dioecious species to study plant sex determination and sex chromosome evolution. To improve our understanding of its genome composition, especially with respect to transposable elements (TEs), which make up the majority of the genome, we performed Illumina HiSeq2000 sequencing of both male and female asparagus genomes followed by bioinformatics analysis. We generated 17 Gb of sequence (12×coverage) and assembled them into 163,406 scaffolds with a total cumulated length of 400 Mbp, which represent about 30% of asparagus genome. Overall, TEs masked about 53% of the A. officinalis assembly. Majority of the identified TEs belonged to LTR retrotransposons, which constitute about 28% of genomic DNA, with Ty1/copia elements being more diverse and accumulated to higher copy numbers than Ty3/gypsy. Compared with LTR retrotransposons, non-LTR retrotransposons and DNA transposons were relatively rare. In addition, comparison of the abundance of the TE groups between male and female genomes showed that the overall TE composition was highly similar, with only slight differences in the abundance of several TE groups, which is consistent with the relatively recent origin of asparagus sex chromosomes. This study greatly improves our knowledge of the repetitive sequence construction of asparagus, which facilitates the identification of TEs responsible for the early evolution of plant sex chromosomes and is helpful for further studies on this dioecious plant. PMID:24810432

  2. Brachycorynella asparagi (Mordv. Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Directory of Open Access Journals (Sweden)

    Beata Borowiak-Sobkowiak

    2016-10-01

    Full Text Available The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis leaves after Brachycorynella asparagi (B. asparagi infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•− and hydrogen peroxide (H2O2 in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age.

  3. Brachycorynella asparagi (Mordv.) Induced—Oxidative Stress and Antioxidative Defenses of Asparagus officinalis L.

    Science.gov (United States)

    Borowiak-Sobkowiak, Beata; Woźniak, Agnieszka; Bednarski, Waldemar; Formela, Magda; Samardakiewicz, Sławomir; Morkunas, Iwona

    2016-01-01

    The aim of this study was to investigate whether and to what extent oxidative stress is induced in leaves of one- and two-month-old plants of Asparagus officinalis L. cv. Argenteuil infested by Brachycorynella asparagi (Mordvilko) at a varied population size. The pest B. asparagi has been described as the most damaging species feeding on asparagus. Analyses using electron paramagnetic resonance (EPR) demonstrated generally higher concentrations of semiquinone radicals with g-values of 2.0045 ± 0.0005 and 2.0026 ± 0.0005 in Asparagus officinalis (A. officinalis) leaves after Brachycorynella asparagi (B. asparagi) infestation than in the control. Observations of leaves under a confocal microscope showed a post-infestation enhanced generation of the superoxide anion radical (O2•−) and hydrogen peroxide (H2O2) in comparison to the control. Strong fluctuations in Mn2+ ion levels detected by EPR spectroscopy versus time were detected in leaves infested by aphids, which may indicate the involvement of these ions in the control of O2•− production. An enhanced superoxide dismutase activity is an important element in leaf defense against oxidative stress. Visible symptoms were found in aphid-infested A. officinalis. Damage to leaves of one- and two-month-old A. officinalis plants by the aphid B. asparagi was dependent on the intensity, duration of infestation and plant age. PMID:27775613

  4. Genome-wide identification and validation of simple sequence repeats (SSRs) from Asparagus officinalis.

    Science.gov (United States)

    Li, Shufen; Zhang, Guojun; Li, Xu; Wang, Lianjun; Yuan, Jinhong; Deng, Chuanliang; Gao, Wujun

    2016-06-01

    Garden asparagus (Asparagus officinalis), an important vegetable cultivated worldwide, can also serve as a model dioecious plant species in the study of sex determination and sex chromosome evolution. However, limited DNA marker resources have been developed and used for this species. To expand these resources, we examined the DNA sequences for simple sequence repeats (SSRs) in 163,406 scaffolds representing approximately 400 Mbp of the A. officinalis genome. A total of 87,576 SSRs were identified in 59,565 scaffolds. The most abundant SSR repeats were trinucleotide and tetranucleotide, accounting for 29.2 and 29.1% of the total SSRs, respectively, followed by di-, penta-, hexa-, hepta-, and octanucleotides. The AG motif was most common among dinucleotides and was also the most frequent motif in the entire A. officinalis genome, representing 14.7% of all SSRs. A total of 41,917 SSR primers pairs were designed to amplify SSRs. Twenty-two genomic SSR markers were tested in 39 asparagus accessions belonging to ten cultivars and one accession of Asparagus setaceus for determination of genetic diversity. The intra-species polymorphism information content (PIC) values of the 22 genomic SSR markers were intermediate, with an average of 0.41. The genetic diversity between the ten A. officinalis cultivars was low, and the UPGMA dendrogram was largely unrelated to cultivars. It is here suggested that the sex of individuals is an important factor influencing the clustering results. The information reported here provides new information about the organization of the microsatellites in A. officinalis genome and lays a foundation for further genetic studies and breeding applications of A. officinalis and related species. PMID:26987412

  5. Effect of the gamma radiation in the metabolic activity of the apical meristem of asparagus (Asparagus officinalis); Efecto de la radiacion gamma en la actividad metabolica del meristemo apical de esparrago (Asparagus officinalis)

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez E, A.; Orozco A, J.A.; Troncoso R, R.; Ojeda C, A.J.; Mercado R, J.N.; Gardea B, A.; Tiznado H, M.E. [Centro de Investigacion en Alimentacion y Desarrollo, A.C. Carretera a la Victoria km. 0.6, A.P. 1735, Hermosillo, Sonora (Mexico); Melendrez A, R. [Universidad de Sonora, A.P. 5-088, Hermosillo, Sonora (Mexico)]. e-mail: aestrada@cascabel.ciad.mx

    2007-07-01

    The asparagus (Asparagus officinalis) is an agricultural product whose production generates a great quantity of wages as well as foreign currencies for the country because a great part of its production is exported to international markets. In direct reason to the high metabolic activity of the apical meristem, this product it possesses a short shelf life under good conditions of commercialization. Due to the above mentioned, the present work had as objective to evaluate the effect of the gamma radiation in the metabolic activity of the apical meristem of turions of asparagus during the postharvest. Turions of asparagus variety Brock of standard quality was treated with gamma radiation to absorbed dose of 1.0, 1.5, 2.0, 2.5 and 3.0 kGy using an irradiator of {sup 60}Co and stored in one controlled temperature camera maintained at 2 C during 8 days. During the experiment, the camera stayed in darkness and under conditions of high relative humidity by means of the water aspersion. Immediately before the one treatment and after 1, 2, 4 and 8 days of storage, the asparagus were sampled to evaluate the breathing speed (VRCG) by means of gas chromatography and scanning differential calorimetry (VRCDB), ethylene production (PE) by means of gas chromatography and production of metabolic heat of the apical meristem of the asparagus by means of scanning differential calorimetry (Q). Its were not found effects due to the gamma radiation in the variables of VRCG, VRCDB and Q. However, for the PE case, it was found that the doses of 1.5 and 2.5 reduced the PE from the first day of storage, while the 3 kGy dose achievement to eliminate completely the ethylene production from the first day of storage. It was concluded that the gamma radiation at the used levels in the present experiment doesn't reduce the metabolic activity of the apical meristem of the turion of asparagus although it can to improve the postharvest quality from the asparagus when reducing the ethylene

  6. Comparative genomic analyses in Asparagus.

    Science.gov (United States)

    Kuhl, Joseph C; Havey, Michael J; Martin, William J; Cheung, Foo; Yuan, Qiaoping; Landherr, Lena; Hu, Yi; Leebens-Mack, James; Town, Christopher D; Sink, Kenneth C

    2005-12-01

    Garden asparagus (Asparagus officinalis L.) belongs to the monocot family Asparagaceae in the order Asparagales. Onion (Allium cepa L.) and Asparagus officinalis are 2 of the most economically important plants of the core Asparagales, a well supported monophyletic group within the Asparagales. Coding regions in onion have lower GC contents than the grasses. We compared the GC content of 3374 unique expressed sequence tags (ESTs) from A. officinalis with Lycoris longituba and onion (both members of the core Asparagales), Acorus americanus (sister to all other monocots), the grasses, and Arabidopsis. Although ESTs in A. officinalis and Acorus had a higher average GC content than Arabidopsis, Lycoris, and onion, all were clearly lower than the grasses. The Asparagaceae have the smallest nuclear genomes among all plants in the core Asparagales, which typically have huge genomes. Within the Asparagaceae, European Asparagus species have approximately twice the nuclear DNA of that of southern African Asparagus species. We cloned and sequenced 20 genomic amplicons from European A. officinalis and the southern African species Asparagus plumosus and observed no clear evidence for a recent genome doubling in A. officinalis relative to A. plumosus. These results indicate that members of the genus Asparagus with smaller genomes may be useful genomic models for plants in the core Asparagales. PMID:16391674

  7. Effects of Asparagus officinalis extracts on liver cell toxicity and ethanol metabolism.

    Science.gov (United States)

    Kim, B-Y; Cui, Z-G; Lee, S-R; Kim, S-J; Kang, H-K; Lee, Y-K; Park, D-B

    2009-09-01

    Asparagus officinalis is a vegetable that is widely consumed worldwide and has also long been used as a herbal medicine for the treatment of several diseases. Although A. officinalis is generally regarded as a supplement for the alleviation of alcohol hangover, little is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the young shoots and the leaves of asparagus and to compare their biochemical properties. The amino acid and inorganic mineral contents were found to be much higher in the leaves than the shoots. In addition, treatment of HepG2 human hepatoma cells with the leaf extract suppressed more than 70% of the intensity of hydrogen peroxide (1 mM)-stimulated DCF fluorescence, a marker of reactive oxygen species (ROS). Cellular toxicities induced by treatment with hydrogen peroxide, ethanol, or tetrachloride carbon (CCl(4)) were also significantly alleviated in response to treatment with the extracts of A. officinalis leaves and shoots. Additionally, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by more than 2-fold in response to treatment with the leaf- and shoot extracts. Taken together, these results provide biochemical evidence of the method by which A. officinalis exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults. Moreover, the results of this study indicate that portions of asparagus that are typically discarded, such as the leaves, have therapeutic use. PMID:19895471

  8. Bioactive compounds, antioxidant and binding activities and spear yield of Asparagus officinalis L.

    Science.gov (United States)

    Lee, Jong Won; Lee, Jeong Hyun; Yu, In Ho; Gorinstein, Shela; Bae, Jong Hyang; Ku, Yang Gyu

    2014-06-01

    The aim of this investigation was to find a proper harvesting period and establishing fern number, which effects the spear yield, bioactive compounds and antioxidant activities of Asparagus officinalis L. Spears were harvested at 2, 4, and 6 weeks after sprouting. Control for comparison was used without harvest. Spears and total yield increased with prolonged spear harvest period. In harvest of 6 weeks long optimum spear yield was the highest and fern numbers were 5 ~ 8. Bioactive compounds (polyphenols, flavonoids, flavanols, tannins and ascorbic acid) and the levels of antioxidant activities by ferric-reducing/antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays in asparagus ethanol extracts significantly differed in the investigated samples and were the highest at 6 weeks harvest period (P asparagus were studied by the interaction of polyphenol ethanol extracts with HSA, using 3D- FL. In conclusion, antioxidant status (bioactive compounds, binding and antioxidant activities) improved with the harvesting period and the first segment from spear tip. Appropriate harvesting is effective for higher asparagus yield and its bioactivity.

  9. Bioactive compounds, antioxidant and binding activities and spear yield of Asparagus officinalis L.

    Science.gov (United States)

    Lee, Jong Won; Lee, Jeong Hyun; Yu, In Ho; Gorinstein, Shela; Bae, Jong Hyang; Ku, Yang Gyu

    2014-06-01

    The aim of this investigation was to find a proper harvesting period and establishing fern number, which effects the spear yield, bioactive compounds and antioxidant activities of Asparagus officinalis L. Spears were harvested at 2, 4, and 6 weeks after sprouting. Control for comparison was used without harvest. Spears and total yield increased with prolonged spear harvest period. In harvest of 6 weeks long optimum spear yield was the highest and fern numbers were 5 ~ 8. Bioactive compounds (polyphenols, flavonoids, flavanols, tannins and ascorbic acid) and the levels of antioxidant activities by ferric-reducing/antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays in asparagus ethanol extracts significantly differed in the investigated samples and were the highest at 6 weeks harvest period (P asparagus were studied by the interaction of polyphenol ethanol extracts with HSA, using 3D- FL. In conclusion, antioxidant status (bioactive compounds, binding and antioxidant activities) improved with the harvesting period and the first segment from spear tip. Appropriate harvesting is effective for higher asparagus yield and its bioactivity. PMID:24793354

  10. The effect of Asparagus officinalis polysaccharide on erythrocyte of S180 mice played in immunological reaction of lymphocytes

    Institute of Scientific and Technical Information of China (English)

    JI Chen-feng; MIN Yong-cui; JI Yu-bin

    2008-01-01

    Objective To contrast the roles of Asparagus officinalis polysaccharide on erythroeyte of S180 mice played in immunological reaction of lymphocytes. To study the effect of Asparagus officinalis polysaecharide on the erythroeyte function of S180 mice. Methods Suspensions of lymphocytes (1×106/mL) and autologous plasma were respectively separated from anticoaguted whole blood of healthy mice with the lymphocyte separation medium. The erythrocytes (1×108/mL) were separated from whole blood of Asparagus officinalis polysaccharide mice. Using the autologous plasm as reactive medium, the role of erythrocytes in regulating the immunological reaction of lymphocytes was appraised. The expression of CD25 on lymphocytes was detected using flow cytometry. Results The immunogical regulating ability of erythrocyte in mice with control groups is much lower than that of normal groups, and the immunogical regulating ability of erythroeyte in mice with Asparagus officinalis polysaccharide groups is much higher than that of control groups. Conclusions According to the effects of erythrocyte CD35 on the immuno-response of lymphocyte and the different of the expression of CD25 on lymphocytes, we prove that Asparagus officinalis polysaecharide can improve the erythrocyte function of S180 mice.

  11. [Cloning and analyzing of the female-specific marker in the dioecious species Asparagus officinalis L].

    Science.gov (United States)

    Lu, Long Dou; Li, Rui Li; Gao, Wu Jun; Deng, Chuan Liang; Wang, Lian Jun

    2006-06-01

    Sex-linked molecular markers are being obtained, which would be essential to be used in the screening of different sex of dioecious plants at the seedling stage. Furthermore, it is important in cloning the gene related to the sex. In this study the random amplified polymorphic DNA (RAPD) technique was employed with the objective to find markers linked to sex determination in Asparagus. A total of 100 primers were tested with the same PCR cycling procedure. A female-associated fragment with a length of about 867bp was generated with S12 primer. The fragment was cloned and sequenced, showing it is abundant in AT and contains 2 shorter open reading frames. In order to convert the RAPD marker into SCAR (sequence characterized amplified regions) marker, 24bp specific primers were constructed and used for PCR amplifying. The female-linked dominant SCAR marker was obtained, which would be efficient to identify the different sex of Asparagus officinalis L. PMID:16944605

  12. Preparation of cellulose nanocrystals from asparagus (Asparagus officinalis L.) and their applications to palm oil/water Pickering emulsion.

    Science.gov (United States)

    Wang, Wenhang; Du, Guanhua; Li, Cong; Zhang, Hongjie; Long, Yunduo; Ni, Yonghao

    2016-10-20

    Nano cellulosic materials as promising emulsion stabilizers have attracted great interest in food industry. In this paper, five different sized cellulose nanocrystals (CNC) samples were prepared from stem of Asparagus officinalis L. using the same sulfuric acid hydrolysis conditions but different times (1.5, 2, 2.5, 3.0, and 3.5h). The sizes of these CNC ranged from 178.2 to 261.8nm, with their crystallinity of 72.4-77.2%. The CNC aqueous dispersions showed a typical shear thinning behavior. In a palm oil/water (30/70, v/v) model solution, stable Pickering emulsions were formed with the addition of CNC, and their sizes are in the range of 1-10μm based on the optical and confocal laser scanning microscopy (CLSM) observation. The CNC sample prepared at 3h hydrolysis time, showed a relative efficient emulsion capacity for palm oil droplets, among these CNCs. Other parameters including the CNC, salt, and casein concentrations on the emulsion stability were studied.

  13. Preparation of cellulose nanocrystals from asparagus (Asparagus officinalis L.) and their applications to palm oil/water Pickering emulsion.

    Science.gov (United States)

    Wang, Wenhang; Du, Guanhua; Li, Cong; Zhang, Hongjie; Long, Yunduo; Ni, Yonghao

    2016-10-20

    Nano cellulosic materials as promising emulsion stabilizers have attracted great interest in food industry. In this paper, five different sized cellulose nanocrystals (CNC) samples were prepared from stem of Asparagus officinalis L. using the same sulfuric acid hydrolysis conditions but different times (1.5, 2, 2.5, 3.0, and 3.5h). The sizes of these CNC ranged from 178.2 to 261.8nm, with their crystallinity of 72.4-77.2%. The CNC aqueous dispersions showed a typical shear thinning behavior. In a palm oil/water (30/70, v/v) model solution, stable Pickering emulsions were formed with the addition of CNC, and their sizes are in the range of 1-10μm based on the optical and confocal laser scanning microscopy (CLSM) observation. The CNC sample prepared at 3h hydrolysis time, showed a relative efficient emulsion capacity for palm oil droplets, among these CNCs. Other parameters including the CNC, salt, and casein concentrations on the emulsion stability were studied. PMID:27474537

  14. Possible regulatory role of phenylalanine ammonia-lyase in the production of anthocyanins in asparagus (Asparagus officinalis L)

    NARCIS (Netherlands)

    Flores, F.B.; Oosterhaven, J.; Martinez-Madrid, M.C.; Romojaro, F.

    2005-01-01

    The regulatory role of phenylalanine ammonia-lyase (PAL) in the light-induced accumulation of anthocyanins in the epidermis of asparagus spears has been analysed. A correlation between the stimulation of PAL activity and the rise in total anthocyanin content has been observed. Light radiation induce

  15. Comparative analysis of gene expression by microarray analysis of male and female flowers of Asparagus officinalis.

    Science.gov (United States)

    Gao, Wu-Jun; Li, Shu-Fen; Zhang, Guo-Jun; Wang, Ning-Na; Deng, Chuan-Liang; Lu, Long-Dou

    2013-01-01

    To identify rapidly a number of genes probably involved in sex determination and differentiation of the dioecious plant Asparagus officinalis, gene expression profiles in early flower development for male and female plants were investigated by microarray assay with 8,665 probes. In total, 638 male-biased and 543 female-biased genes were identified. These genes with biased-expression for male and female were involved in a variety of processes associated with molecular functions, cellular components, and biological processes, suggesting that a complex mechanism underlies the sex development of asparagus. Among the differentially expressed genes involved in the reproductive process, a number of genes associated with floral development were identified. Reverse transcription-PCR was performed for validation, and the results were largely consistent with those obtained by microarray analysis. The findings of this study might contribute to understanding of the molecular mechanisms of sex determination and differentiation in dioecious asparagus and provide a foundation for further studies of this plant.

  16. Comparative analysis of gene expression by microarray analysis of male and female flowers of Asparagus officinalis.

    Science.gov (United States)

    Gao, Wu-Jun; Li, Shu-Fen; Zhang, Guo-Jun; Wang, Ning-Na; Deng, Chuan-Liang; Lu, Long-Dou

    2013-01-01

    To identify rapidly a number of genes probably involved in sex determination and differentiation of the dioecious plant Asparagus officinalis, gene expression profiles in early flower development for male and female plants were investigated by microarray assay with 8,665 probes. In total, 638 male-biased and 543 female-biased genes were identified. These genes with biased-expression for male and female were involved in a variety of processes associated with molecular functions, cellular components, and biological processes, suggesting that a complex mechanism underlies the sex development of asparagus. Among the differentially expressed genes involved in the reproductive process, a number of genes associated with floral development were identified. Reverse transcription-PCR was performed for validation, and the results were largely consistent with those obtained by microarray analysis. The findings of this study might contribute to understanding of the molecular mechanisms of sex determination and differentiation in dioecious asparagus and provide a foundation for further studies of this plant. PMID:23748756

  17. Steroids from the Roots of Asparagus officinalis and Their Cytotoxic Activity

    Institute of Scientific and Technical Information of China (English)

    Xue-Feng Huang; Yu-Ying Lin; Ling-Yi Kong

    2008-01-01

    One new (Sarsasapogenin O) and seven known steroids were isolated from the roots of Asparagus officinalis L. Their structures were elucidated on the basis of spectroscopic analysis, including various 2D-NMR techniques, hydrolysis,and by comparison of spectral data of known compounds. These compounds together with nine steroids which were previously isolated from this plant, were tested for cytotoxic activity. Among them, eight compounds displayed significant cytotoxicities against human A2780, HO-8910, Eca-109, MGC-803, CNE, LTEP-a-2, KB and mouse L1210 tumor cells.

  18. A new steroidal saponin from the dried stems of Asparagus officinalis L.

    Science.gov (United States)

    Sun, Zhouxuan; Huang, Xuefeng; Kong, Lingyi

    2010-04-01

    Yamogenin II (1), a new steroidal saponin with a unique aglycone moiety, and (25S)-spirostan-5-ene-3beta-ol-3-O-alpha-L-rhamnopyranosyl-(1,2)-[alpha-L-rhamnopyranosyl-(1,4)]-beta-D-glucopyranoside (2), were isolated from the dried stems of Asparagus officinalis L. The structure of 1 was assessed by spectroscopial analysis as (25S)-spirostan-5-ene-3beta,21-diol-3-O-alpha-L-rhamnopyranosyl-(1,2)-[alpha-L-rhamnopyranosyl-(1,4)]-beta-D-glucopyranoside. PMID:19751809

  19. Study regarding to Asparagus officinalis L. calus reaction, subcultured on aseptic medium with various growth regulators

    Directory of Open Access Journals (Sweden)

    Floare HURGOIU

    2008-05-01

    Full Text Available In our studies, we investigated the effects of some growth regulators, in aseptic conditions, on obtaining Asparagus officinalis plantlets from calus, after 12 weeks of its inoculation.The most recomanded culture media, for obtaining a high number of multimodal stems was MS basal medium, with a mixture of 1mg/l BA and 1 mg/l IBA, which inducing a considerably calusogenesis, and a lot of regenerated plantlets from it, but finally, this medium-after 12 weeks of culture initiation, induced plantlets vitrification.

  20. Steroids from the roots of Asparagus officinalis and their cytotoxic activity.

    Science.gov (United States)

    Huang, Xue-Feng; Lin, Yu-Ying; Kong, Ling-Yi

    2008-06-01

    One new (Sarsasapogenin O) and seven known steroids were isolated from the roots of Asparagus officinalis L. Their structures were elucidated on the basis of spectroscopic analysis, including various 2D-NMR techniques, hydrolysis, and by comparison of spectral data of known compounds. These compounds together with nine steroids which were previously isolated from this plant, were tested for cytotoxic activity. Among them, eight compounds displayed significant cytotoxicities against human A2780, HO-8910, Eca-109, MGC-803, CNE, LTEP-a-2, KB and mouse L1210 tumor cells. PMID:18713412

  1. Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology.

    Science.gov (United States)

    Zhao, Qingsheng; Kennedy, John F; Wang, Xiaodong; Yuan, Xiaofan; Zhao, Bing; Peng, Youshun; Huang, Yunxiang

    2011-08-01

    Polysaccharides were extracted from Asparagus officinalis. A novel ultrasonic circulating extraction (UCE) technology was applied for the polysaccharide extraction. Three-factor-three-level Box-Behnken design was employed to optimize ultrasonic power, extraction time and the liquid-solid ratio to obtain a high polysaccharide yield. The optimal extraction conditions were as follows: ultrasonic power was 600 W, extraction time was 46 min, the liquid-solid ratio was 35 mL/g. Under these conditions, the experimental yield of polysaccharides was 3.134%, which was agreed closely to the predicted value. The average molecular weight of A. officinalis polysaccharide was about 6.18×10(4) Da. The polysaccharides were composed of glucose, fucose, arabinose, galactose and rhamnose in a ratio of 2.18:1.86:1.50:0.98:1.53. Compared with hot water extraction (HWE), UCE showed time-saving, higher yield and no influence on the structure of asparagus polysaccharides. The results indicated that ultrasonic circulating extraction technology could be an effective and advisable technique for the large scale production of plant polysaccharides. PMID:21549748

  2. Plant regeneration after long-term callus culture in clones of Asparagus officinalis L.

    Science.gov (United States)

    Pontaroli, A C; Camadro, E L

    2005-12-01

    Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg x l(-1) kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg x l(-1) 2,4-D and 1 mg x l(-1) kinetin. Shoot primordia (SP) induction from > 18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone "265" on MS medium with 5 mg x l(-1) 2iP, 1 mg x l(-1) IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone '265' on MS medium with 0.1 mg x l(-1) kinetin, 0.1 mg x l(-1) NAA, 1.32 mg x l(-1) ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes. PMID:16524253

  3. Assessment of in vitro digestibility and fermentation parameters of alfalfa hay-based diet following direct incorporation of fenugreek seed (Trigonella foenum) and asparagus root (Asparagus officinalis).

    Science.gov (United States)

    Naseri, V; Hozhabri, F; Kafilzadeh, F

    2013-08-01

    This study was completed to evaluate the effect of fenugreek seed (Trigonella foenum; FS) and asparagus root (Asparagus officinalis; AR) on in vitro nutrient digestibility and fermentation patterns. Different levels [0%, 5%, 10%, 15% and 20% of dry matter (DM)] of the medicinal plants were included using alfalfa hay (AH) as a basal substrate at different incubation times (12, 18, 24 and 48 h). Total phenolic components of AH, FS and AR were 5.9, 10 and 8.3 g/kg DM, whereas total tannins were 0.4, 3.8 and 1.5 g/kg DM, respectively. Corresponding values for saponins were 10.4, 27.3 and 40.3 g/kg DM. Fenugreek seed increased (pAsparagus root also increased (p<0.05) in vitro OM digestibility and decreased (p<0.05) CP digestibility at different incubation times. Neutral detergent fibre digestibility was increased (p<0.05) by the addition of AR or FS at low levels, but decreased (p<0.05) noticeably by increasing level of two plants in the basal substrate. Ammonia-N concentration was markedly reduced (p<0.05) by the addition of AR at different incubation times, and this reduction was accompanied by the decrease in CP digestibility. True DM degradability and partitioning factor (ratio of substrate DM truly degraded to gas volume produced at different times of incubation) were increased, and total volatile fatty acid concentration and total gas production were decreased (p<0.05) with the addition of FS (at 10% and 15% DM levels) or AR (at 5%, 10% and 15% DM levels) at different incubation times. Results suggest that FS and AR may have potential as feed additives to increase the efficiency of nutrients' utilization, particularly of nitrogen in ruminant diets.

  4. Assessment of in vitro digestibility and fermentation parameters of alfalfa hay-based diet following direct incorporation of fenugreek seed (Trigonella foenum) and asparagus root (Asparagus officinalis).

    Science.gov (United States)

    Naseri, V; Hozhabri, F; Kafilzadeh, F

    2013-08-01

    This study was completed to evaluate the effect of fenugreek seed (Trigonella foenum; FS) and asparagus root (Asparagus officinalis; AR) on in vitro nutrient digestibility and fermentation patterns. Different levels [0%, 5%, 10%, 15% and 20% of dry matter (DM)] of the medicinal plants were included using alfalfa hay (AH) as a basal substrate at different incubation times (12, 18, 24 and 48 h). Total phenolic components of AH, FS and AR were 5.9, 10 and 8.3 g/kg DM, whereas total tannins were 0.4, 3.8 and 1.5 g/kg DM, respectively. Corresponding values for saponins were 10.4, 27.3 and 40.3 g/kg DM. Fenugreek seed increased (pAsparagus root also increased (p<0.05) in vitro OM digestibility and decreased (p<0.05) CP digestibility at different incubation times. Neutral detergent fibre digestibility was increased (p<0.05) by the addition of AR or FS at low levels, but decreased (p<0.05) noticeably by increasing level of two plants in the basal substrate. Ammonia-N concentration was markedly reduced (p<0.05) by the addition of AR at different incubation times, and this reduction was accompanied by the decrease in CP digestibility. True DM degradability and partitioning factor (ratio of substrate DM truly degraded to gas volume produced at different times of incubation) were increased, and total volatile fatty acid concentration and total gas production were decreased (p<0.05) with the addition of FS (at 10% and 15% DM levels) or AR (at 5%, 10% and 15% DM levels) at different incubation times. Results suggest that FS and AR may have potential as feed additives to increase the efficiency of nutrients' utilization, particularly of nitrogen in ruminant diets. PMID:22741923

  5. Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

    OpenAIRE

    Fan, Rui; Yuan, Fang; Wang, Ning; Gao, Yanxiang; Huang, Yunxiang

    2014-01-01

    Asparagus residues were used as materials to obtain antioxidant compounds by solid–liquid extraction in this study. The effects of different extraction parameters including extraction solvents, time, temperature and liquid–solid ratio on the contents of total flavonoids, total phenolics and total antioxidant activity were investigated. Antioxidant activity of the extract from asparagus residues was evaluated by HPLC- ABTS · + and the bioactive components were identified by HPLC- MS/MS. The re...

  6. Hypolipidemic effect of n-butanol Extract from Asparagus officinalis L. in mice fed a high-fat diet.

    Science.gov (United States)

    Zhu, Xinglei; Zhang, Wen; Pang, Xiufeng; Wang, Jiesi; Zhao, Jingjing; Qu, Weijing

    2011-08-01

    During industrial processing of Asparagus (Asparagus officinalis L.), around half of each spear is discarded. However, these discarded asparagus (by-products) might be used as food supplements for their potential therapeutic effects. This study evaluated the hypolipidemic effect of n-butanol extract (BEA) from asparagus by-products in mice fed a high-fat diet (HFD). Continuous HFD feeding caused hyperlipidemia, oxidative stress and liver damage in mice. Interestingly, while BEA significantly decreased the levels of body weight gain, serum total cholesterol and low density lipoprotein cholesterol, it dramatically increased the high density lipoprotein level when administered at three different doses (40, 80 or 160 mg/kg body weight) for 8 weeks in hyperlipidemic mice. In addition, BEA decreased the levels of alanine transaminase, aspartate transaminase and alkaline phosphatase in serum. Finally, superoxide dismutase activity and the total antioxidation capacity were evidently increased, while the malondialdehyde level and the distribution of lipid droplets were reduced in liver cells of BEA-treated mice. Taken together, the findings of this study suggested that BEA had a strong hypolipidemic function and could be used as a supplement in healthcare foods and drugs or in combination with other hypolipidemic drugs.

  7. Hypolipidemic effect of n-butanol Extract from Asparagus officinalis L. in mice fed a high-fat diet.

    Science.gov (United States)

    Zhu, Xinglei; Zhang, Wen; Pang, Xiufeng; Wang, Jiesi; Zhao, Jingjing; Qu, Weijing

    2011-08-01

    During industrial processing of Asparagus (Asparagus officinalis L.), around half of each spear is discarded. However, these discarded asparagus (by-products) might be used as food supplements for their potential therapeutic effects. This study evaluated the hypolipidemic effect of n-butanol extract (BEA) from asparagus by-products in mice fed a high-fat diet (HFD). Continuous HFD feeding caused hyperlipidemia, oxidative stress and liver damage in mice. Interestingly, while BEA significantly decreased the levels of body weight gain, serum total cholesterol and low density lipoprotein cholesterol, it dramatically increased the high density lipoprotein level when administered at three different doses (40, 80 or 160 mg/kg body weight) for 8 weeks in hyperlipidemic mice. In addition, BEA decreased the levels of alanine transaminase, aspartate transaminase and alkaline phosphatase in serum. Finally, superoxide dismutase activity and the total antioxidation capacity were evidently increased, while the malondialdehyde level and the distribution of lipid droplets were reduced in liver cells of BEA-treated mice. Taken together, the findings of this study suggested that BEA had a strong hypolipidemic function and could be used as a supplement in healthcare foods and drugs or in combination with other hypolipidemic drugs. PMID:21280112

  8. Production of extracellular exoinulinase from Kluyveromyces marxianus YS-1 using root tubers of Asparagus officinalis.

    Science.gov (United States)

    Singh, R S; Bhermi, H K

    2008-10-01

    Root tubers of Asparagus officinalis were used as a source of raw inulin for the production of exoinulinase (EC 3.2.1.7) from Kluyveromyces marxianus YS-1. Root extract prepared at 10kg/cm2 pressure for 10min showed maximum inulinase production. Medium components and process parameters were standardized to improve the enzyme production. Inulinase yield of 40.2IU/mL in a medium containing raw inulin (3.5%), beef extract (2%), SDS (0.001%), Mn2+ (2.0mM), Mg2+ (1.5mM), Co2+ (2mM) and pH 6.5 has been obtained under agitation (150rpm) after 60h of incubation at 30 degrees C at shake flask level. After optimization, the enzyme production was 4.8 times more than the basal medium. To test the feasibility of raw inulin from A. officinalis for the production of inulinase, trials were also made in a bioreactor (1.5L). Inulinase activity of 50.2IU/mL was obtained from raw inulin (4.0%) under agitation (200rpm) and aeration (0.75vvm) at 30 degrees C after 60h of fermentation. Inulinase yield in bioreactor was almost six times higher than the basal medium used initially in shake flask. PMID:18280145

  9. Chemical, Physicochemical and Functional Characteristics of Dietary Fiber Obtained From Asparagus byproducts (Asparagus officinalis L.) / Características Químicas, Físicoquímicas y Funcionales de la Fibra Dietaria Obtenida de los Subproductos del Espá

    OpenAIRE

    Edith Lorena Agudelo Cadavid; Diego Alonso Restrepo Molina; José Régulo Cartagena Valenzuela

    2014-01-01

    Abstract. Due to its mechanical and biochemical properties,dietary fiber is part of a healthy diet. Containing good levels ofprebiotics, asparagus (Asparagus officinalis L.) produces healthyeffects when incorporated into processed foods. The objective ofthe current study was to obtain fiber from asparagus byproductsand determine its chemical composition [Total Dietary Fiber (TDF),protein, moisture and ash)] and physicochemical [pH, and wateractivity (aw)] and functional characteristics [Water...

  10. Progress on Study of Anti-tumor Effect of Asparagus Officinalis%芦笋抗肿瘤作用研究进展

    Institute of Scientific and Technical Information of China (English)

    尚明

    2013-01-01

    目的:芦笋是天门冬科天门冬属多年生草本植物,具有极高的营养价值,含有大量的营养物质和活性成分,同时具有多种生理活性,该文综述了芦笋抗肿瘤作用的研究进展。%Objective:Asparagus officinalis is a perennial herb plant, and it has abundant nutrition and many active components such as polysaccharide, saponin, flavonoids, tissue protein, and trace element. Many scientific studies showed Asparagus officinalis had many activities. In this paper, we summarize the progress on the study of anti-tumor effect of Asparagus officinalis.

  11. Ambispora granatensis, a new arbuscular mycorrhizal fungus, associated with Asparagus officinalis in Andalucia (Spain).

    Science.gov (United States)

    Palenzuela, Javier; Barea, José-Miguel; Ferrol, Nuria; Oehl, Fritz

    2011-01-01

    A new dimorphic fungal species in the arbuscular mycorrhiza-forming Glomeromycota, Ambispora granatensis, was isolated from an agricultural site in the province of Granada (Andalucía, Spain) growing in the rhizosphere of Asparagus officinalis. It was propagated in pot cultures with Trifolium pratense and Sorghum vulgare. The fungus also colonized Ri T-DNA transformed Daucus carota roots but did not form spores in these root organ cultures. The spores of the acaulosporoid morph are 90-150 μm diam and hyaline to white to pale yellow. They have three walls and a papillae-like rough irregular surface on the outer surface of the outer wall. The irregular surface might become difficult to detect within a few hours in lactic acid-based mountings but are clearly visible in water. The structural central wall layer of the outer wall is only 0.8-1.5 μm thick. The glomoid spores are formed singly or in small, loose spore clusters of 2-10 spores. They are hyaline to pale yellow, (25)40-70 μm diam and have a bilayered spore wall without ornamentation. Nearly full length sequences of the 18S and the ITS regions of the ribosomal gene place the new fungus in a separate clade next to Ambispora fennica and Ambispora gerdemannii. The acaulosporoid spores of the new fungus can be distinguished easily from all other spores in genus Ambispora by the conspicuous thin outer wall. PMID:20952800

  12. Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures

    Directory of Open Access Journals (Sweden)

    Pontaroli Ana Clara

    2005-01-01

    Full Text Available Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants analyzed. All crosses between one pistillate plant and 35 regenerants, as well as the controls, produced fruits and seeds; however, no plump seeds resulted in 35.3% of the crosses with regenerants, and no seeds germinated in 12.5% of those with apparently normal seeds. Fruit and seed set was similar in crosses with diploid regenerants with normal meiosis and the controls but was lower in crosses with diploid and polyploid regenerants with abnormal meiosis. Our results show that the regenerated plants exhibited conspicuous somaclonal variation that could be eventually exploited for in vitro selection systems.

  13. 芦笋重要真菌病害研究进展%The Advance of Major Fungal Diseases Studies on Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张岳平; 陈光宇; 罗绍春; 瞿华香

    2012-01-01

    Asparagus officinalis L. Is an important high nutrition vegetable worldwide. In present, fungal diseases were the one of the severe limiting factors for Asparagus officinalis L. Production around the world. The major fungal diseases of Asparagus officinalis L. Included stem blight, root rot, Fusarium wilt, and brown spot. Among them, asparagus stem blight was the most devastating disease worldwide, especially in China. The authors of the present paper reviewed the advances in the areas of the physiology characterization and pathogenicity of the major fungal pathogens of Asparagus officinalis L., the host resistance, the key prevent and control technologies, and a prospect for the future was made as well. The mechanism of fungi pathogenisis and host resistance should be strengthened, as was helpful for resistance molecular breeding of Asparagus officinalis L. In future.%芦笋是重要的功能型蔬菜,芦笋真菌病害是威胁其生产的主要瓶颈之一,主要包括茎枯病、根腐病、枯萎病、褐斑病等.其中茎枯病是危害芦笋生产的世界性病害,特别是在中国,已成为生产中的突出问题.笔者简述了茎枯病等芦笋重要真菌病害在病原菌生物学特性、致病性、芦笋品种抗病性及病害关键防控技术等方面的研究进展,并展望了其发展趋势,认为今后应加强病原菌的致病和芦笋的抗病分子机制研究,为开展芦笋抗茎枯病分子育种提供基础.

  14. Enzyme-treated Asparagus officinalis extract shows neuroprotective effects and attenuates cognitive impairment in senescence-accelerated mice.

    Science.gov (United States)

    Sakurai, Takuya; Ito, Tomohiro; Wakame, Koji; Kitadate, Kentaro; Arai, Takashi; Ogasawara, Junetsu; Kizaki, Takako; Sato, Shogo; Ishibashi, Yoshinaga; Fujiwara, Tomonori; Akagawa, Kimio; Ishida, Hitoshi; Ohno, Hideki

    2014-01-01

    Increases in the number of patients with dementia involving Alzheimer's disease (AD) are seen as a grave public health problem. In neurodegenerative disorders involving AD, biological stresses, such as oxidative and inflammatory stress, induce neural cell damage. Asparagus (Asparagus officinalis) is a popular vegetable, and an extract prepared from this reportedly possesses various beneficial biological activities. In the present study, we investigated the effects of enzyme-treated asparagus extract (ETAS) on neuronal cells and early cognitive impairment of senescence-accelerated mouse prone 8 (SAMP8) mice. The expression of mRNAs for factors that exert cytoprotective and anti-apoptotic functions, such as heat-shock protein 70 and heme oxygenase-1, was upregulated in NG108-15 neuronal cells by treatment with ETAS. Moreover, when release of lactate dehydrogenase from damaged NG108-15 cells was increased for cells cultured in medium containing either the nitric oxide donor sodium nitroprusside or the hypoxia mimic reagent cobalt chloride, ETAS significantly attenuated this cell damage. Also, when contextual fear memory, which is considered to be a hippocampus-dependent memory, was significantly impaired in SAMP8 mice, ETAS attenuated the cognitive impairment. These results suggest that ETAS produces cytoprotective effects in neuronal cells and attenuates the effects on the cognitive impairment of SAMP8 mice.

  15. Enzyme-treated Asparagus officinalis extract shows neuroprotective effects and attenuates cognitive impairment in senescence-accelerated mice.

    Science.gov (United States)

    Sakurai, Takuya; Ito, Tomohiro; Wakame, Koji; Kitadate, Kentaro; Arai, Takashi; Ogasawara, Junetsu; Kizaki, Takako; Sato, Shogo; Ishibashi, Yoshinaga; Fujiwara, Tomonori; Akagawa, Kimio; Ishida, Hitoshi; Ohno, Hideki

    2014-01-01

    Increases in the number of patients with dementia involving Alzheimer's disease (AD) are seen as a grave public health problem. In neurodegenerative disorders involving AD, biological stresses, such as oxidative and inflammatory stress, induce neural cell damage. Asparagus (Asparagus officinalis) is a popular vegetable, and an extract prepared from this reportedly possesses various beneficial biological activities. In the present study, we investigated the effects of enzyme-treated asparagus extract (ETAS) on neuronal cells and early cognitive impairment of senescence-accelerated mouse prone 8 (SAMP8) mice. The expression of mRNAs for factors that exert cytoprotective and anti-apoptotic functions, such as heat-shock protein 70 and heme oxygenase-1, was upregulated in NG108-15 neuronal cells by treatment with ETAS. Moreover, when release of lactate dehydrogenase from damaged NG108-15 cells was increased for cells cultured in medium containing either the nitric oxide donor sodium nitroprusside or the hypoxia mimic reagent cobalt chloride, ETAS significantly attenuated this cell damage. Also, when contextual fear memory, which is considered to be a hippocampus-dependent memory, was significantly impaired in SAMP8 mice, ETAS attenuated the cognitive impairment. These results suggest that ETAS produces cytoprotective effects in neuronal cells and attenuates the effects on the cognitive impairment of SAMP8 mice. PMID:24660475

  16. Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort), of Agrostemma githago L. (corn cockle) and of Asparagus officinalis L. (asparagus), and from the latex of Hura crepitans L. (sandbox tree).

    Science.gov (United States)

    Stirpe, F; Gasperi-Campani, A; Barbieri, L; Falasca, A; Abbondanza, A; Stevens, W A

    1983-12-15

    Ribosome-inactivating proteins, similar to those already known [Barbieri & Stirpe (1982) Cancer Surveys 1, 489-520] were purified from the seeds of Saponaria officinalis (two proteins), of Agrostemma githago (three proteins), and of Asparagus officinalis (three proteins), and from the latex of Hura crepitans (one protein). The yield ranged from 8 to 400 mg/100 g of starting material. All proteins have an Mr of approx. 30000 and an alkaline isoelectric point. Their sugar content varies from 0 (proteins from S. officinalis) to 40% (protein from H. crepitans). The ribosome-inactivating proteins inhibit protein synthesis by rabbit reticulocyte lysate, the ID50 (concentration giving 50% inhibition) ranging from 1 ng/ml (a protein from S. officinalis) to 18 ng/ml (a protein from A. githago). Those which were tested (the proteins from S. officinalis and from A. githago) also inhibit polymerization of phenylalanine by isolated ribosomes, acting in an apparently catalytic manner. The protein from H. crepitans inhibited protein synthesis by HeLa cells, with an ID50 of 4 micrograms/ml, whereas the proteins from S. officinalis and from A. githago had an ID50 of more than 50-100 micrograms/ml. The ribosome-inactivating proteins from S. officinalis and from A. githago reduced the number of local lesions by tobacco-mosaic virus in the leaves of Nicotiana glutinosa.

  17. Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

    Science.gov (United States)

    Fan, Rui; Yuan, Fang; Wang, Ning; Gao, Yanxiang; Huang, Yunxiang

    2015-05-01

    Asparagus residues were used as materials to obtain antioxidant compounds by solid-liquid extraction in this study. The effects of different extraction parameters including extraction solvents, time, temperature and liquid-solid ratio on the contents of total flavonoids, total phenolics and total antioxidant activity were investigated. Antioxidant activity of the extract from asparagus residues was evaluated by HPLC- ABTS · (+) and the bioactive components were identified by HPLC- MS/MS. The results showed that the extraction yield was significantly influenced (P < 0.05) by solvent composition, extraction time and temperature. The appropriate parameters were preferred as extraction solvent of 50 % ethanol with liquid-solid ratio of 30:1, extraction temperature of 80 °C and time of 2 h. Antioxidant activity evaluation of the extract indicated flavonoids and phenolics were dominant bioactive compounds. Five antioxidant compounds were identified as ferulic acid, kaempferol, quercetin, rutin and isorhamnetin. PMID:25892766

  18. The Study on Mixed Drink Production from Asparagus officinalis Linu Juice and Aloc Juice%芦笋芦荟复合饮料的研制

    Institute of Scientific and Technical Information of China (English)

    姚晓敏; 顾文祥; 顾蓉芳

    2001-01-01

    利用正交实验设计,对芦笋、芦荟复合饮料制作进行探讨。芦笋汁的制作以芦笋头芽、主茎部分为主,辅以根基部分汁。芦笋的合理杀青条件为0.01mol/L柠檬酸,96℃,4min。芦荟汁中加入Vc、Na2SO3、柠檬酸均可增强其稳定性。芦笋汁和芦荟汁的最佳配比为V(芦笋):V(芦荟)=3:1。糖酸调整如下:加入m(蜂蜜)=10%~30%的蜂蜜,加入苹果酸调pH值为3.5~4。成品风味浓郁,口感柔和,具有保健、清凉止渴之功效。%The production of drink by mixing Asparagus officinalis Linu and aloe juice was studied by orthogonal test. Asparagus officinalis Linu juice was mainly drawing from Asparagus officinalis Linu bud and stem. The rational blanching condition was 0.01mol/L citric acid,96℃, 4 minutes. The stability of aloe juice could be improved by adding Vc, Na2SO3 or citric acid.The best quota of Asparagus officinalis Linu juice and aloe juice was 30% Asparagus officinalis Linu juice,10% aloe juice, 10%~30% honey, pH 3.5~4. The product has heavy flavour, soft taste and healthy function.

  19. Quantitative Analysis of the Migration and Accumulation of Bacillus subtilis in Asparagus officinalis.

    Science.gov (United States)

    Hao, Bian-Qing; Ma, Li-Ping; Qiao, Xiong-Wu

    2015-09-01

    Bacillus subtilis B96-II is a broad-spectrum biological control strain. It effectively suppresses soil-borne fungal diseases in vegetables. A green fluorescence protein (GFP) was expressed in B96-II to detect migration of B96-II into the root and stem of asparagus. The GFP-tagged B96-II (B96-II-GFP) strain exhibited bright green fluorescence under a fluorescence microscope. GFP was stable and had no apparent effects on the growth of the strain. Asparagus plants were planted in the soil inoculated with B96-II-GFP. Our results showed that B96-II-GFP was detected in both the root and stem 15, 30, and 45 days after the asparagus seedlings were planted. B96-II-GFP was also detected in leaves but at a lower concentration. The highest concentration was detected in 15 days, and the number of bacteria decreased subsequently irrespective of duration of growth or sampling period. The highest concentration of B96-II-GFP was present in the root base suggesting that the root base served as the hub of bacterial migration from the soil to the stem.

  20. Quantitative Analysis of the Migration and Accumulation of Bacillus subtilis in Asparagus officinalis.

    Science.gov (United States)

    Hao, Bian-Qing; Ma, Li-Ping; Qiao, Xiong-Wu

    2015-09-01

    Bacillus subtilis B96-II is a broad-spectrum biological control strain. It effectively suppresses soil-borne fungal diseases in vegetables. A green fluorescence protein (GFP) was expressed in B96-II to detect migration of B96-II into the root and stem of asparagus. The GFP-tagged B96-II (B96-II-GFP) strain exhibited bright green fluorescence under a fluorescence microscope. GFP was stable and had no apparent effects on the growth of the strain. Asparagus plants were planted in the soil inoculated with B96-II-GFP. Our results showed that B96-II-GFP was detected in both the root and stem 15, 30, and 45 days after the asparagus seedlings were planted. B96-II-GFP was also detected in leaves but at a lower concentration. The highest concentration was detected in 15 days, and the number of bacteria decreased subsequently irrespective of duration of growth or sampling period. The highest concentration of B96-II-GFP was present in the root base suggesting that the root base served as the hub of bacterial migration from the soil to the stem. PMID:26126832

  1. A PCR-denaturing gradient gel electrophoresis (DGGE) approach to assess Fusarium diversity in asparagus

    NARCIS (Netherlands)

    Yergeau, E.; Filion, M.; Vujanovic, V.; St-Arnaud, M.

    2005-01-01

    In North America, asparagus (Asparagus officinalis) production suffers from a crown and root rot disease mainly caused by Fusarium oxysporum f. sp. asparagi and F. proliferatum. Many other Fusarium species are also found in asparagus fields, whereas accurate detection and identification of these org

  2. Studies on Lignan Components of Asparagus officinalis%石刁柏的木脂素类成分研究

    Institute of Scientific and Technical Information of China (English)

    黄雪峰; 罗俊; 张勇; 孔令义

    2007-01-01

    目的 研究石刁柏(Asparagus officinalis)根茎的化学成分,为阐明其有效成分提供依据.方法 利用硅胶、Sephadex LH-20柱色谱进行分离,根据化合物的光谱数据(IR,UV,MS,1H-NMR,13C-NMR)鉴定其结构.结果 从其乙醇提取物中分离得到4个化合物,分别鉴定为:(+)-nyasd(1),3'-methoxynyasin(2),syringaresind-4′,4″-O-bis-β-D-glucoside(3),syringaresinol-4-O-β-D-glucopyranoside(4).结论 木脂素类化合物1~4均为首次从该植物中分离得到.

  3. Top-down Targeted Metabolomics Reveals a Sulfur-Containing Metabolite with Inhibitory Activity against Angiotensin-Converting Enzyme in Asparagus officinalis.

    Science.gov (United States)

    Nakabayashi, Ryo; Yang, Zhigang; Nishizawa, Tomoko; Mori, Tetsuya; Saito, Kazuki

    2015-05-22

    The discovery of bioactive natural compounds containing sulfur, which is crucial for inhibitory activity against angiotensin-converting enzyme (ACE), is a challenging task in metabolomics. Herein, a new S-containing metabolite, asparaptine (1), was discovered in the spears of Asparagus officinalis by targeted metabolomics using mass spectrometry for S-containing metabolites. The contribution ratio (2.2%) to the IC50 value in the crude extract showed that asparaptine (1) is a new ACE inhibitor.

  4. 石刁柏的种子繁殖方法与栽培管理技术%Seed propagation and planting management technology of the Asparagus officinalis

    Institute of Scientific and Technical Information of China (English)

    曹振领; 张爱辉; 陆彩凤; 张国用

    2002-01-01

    @@ 石刁柏(Asparagus officinalis L.)通常称为芦笋、露笋,为百合科天门冬属多年生草本植物.直立生长,株高可达2m以上(结实植株),茎粗直径最大1.5cm.生育中后期因分枝多,纤细柔弱,头重,常俯垂.

  5. Top-down Targeted Metabolomics Reveals a Sulfur-Containing Metabolite with Inhibitory Activity against Angiotensin-Converting Enzyme in Asparagus officinalis.

    Science.gov (United States)

    Nakabayashi, Ryo; Yang, Zhigang; Nishizawa, Tomoko; Mori, Tetsuya; Saito, Kazuki

    2015-05-22

    The discovery of bioactive natural compounds containing sulfur, which is crucial for inhibitory activity against angiotensin-converting enzyme (ACE), is a challenging task in metabolomics. Herein, a new S-containing metabolite, asparaptine (1), was discovered in the spears of Asparagus officinalis by targeted metabolomics using mass spectrometry for S-containing metabolites. The contribution ratio (2.2%) to the IC50 value in the crude extract showed that asparaptine (1) is a new ACE inhibitor. PMID:25922884

  6. Chemical constituents of Asparagus

    Directory of Open Access Journals (Sweden)

    J S Negi

    2010-01-01

    Full Text Available Asparagus species (family Liliaceae are medicinal plants of temperate Himalayas. They possess a variety of biological properties, such as being antioxidants, immunostimulants, anti-inflammatory, antihepatotoxic, antibacterial, antioxytocic, and reproductive agents. The article briefly reviews the isolated chemical constituents and the biological activities of the plant species. The structural formula of isolated compounds and their distribution in the species studied are also given.

  7. Characterization of asparagus lignin by HPLC.

    Science.gov (United States)

    Jaramillo-Carmona, S; Fuentes-Alventosa, J M; Rodríguez-Gutiérrez, G; Waldron, K W; Smith, A C; Guillén-Bejarano, R; Fernández-Bolaños, J; Jiménez-Araujo, A; Rodríguez-Arcos, R

    2008-09-01

    Lignin is the cell wall component most frequently associated with hardening. Its characterization and quantification are very important to understand the biochemical modifications related to the changes in texture of vegetables such as asparagus (Asparagus officinalis), in which this organoleptic attribute is a very important quality factor. In this study, asparagus lignin from the basal sections of fresh and stored spears was analyzed using 2 methods, the traditional (Klason lignin) and the recently developed derivatization, followed by reductive cleavage (DFRC) method. The latter is a simple and reproducible technique for lignin characterization based on a degradation procedure that produces analyzable monomers and dimers by cleaving alpha- and beta-aryl ethers in lignins. The primary monomers derived from DFRC degradation of lignins are essentially p-coumaryl peracetate, coniferyl peracetate, and sinapyl peracetate. To evaluate the efficiency of the DFRC method, our investigations have been carried on distinct sample types, including wood (data not shown), straw, and asparagus samples. The results have confirmed that lignin composition is affected by plant nature. It has been found that whereas wood samples mostly contain coniferyl units, plant foods, such as straw and asparagus, contain both coniferyl and guaiacyl units. PMID:18803697

  8. 芦笋皂苷的抗肿瘤作用研究进展%Research Advancement on Anti-tumor Activity of Saponins from Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    王洁琼; 赵頔; 冉霞; 瞿伟菁

    2011-01-01

    芦笋是一种常见蔬菜,富含多种营养物质,在多种疾病的预防和治疗中发挥良好的药理效应.芦笋中的甾体皂苷是其生物活性的主要表现物质,现已从芦笋中分离出的皂苷单体有19种.本文概述了它们的来源及结构,对其中已被报道的几种皂苷单体在肿瘤预防和治疗方面的作用、机理及研究进展加以综述,为进一步分离新的芦笋皂苷单体及其对肿瘤的预防和治疗提供参考.%Asparagus officinalis L.is a common green vegetable, rich in a variety of nutrients, and possesses pharmacological effects on the prevention and treatment of several diseases.The saponins are the main active component of Asparagus officinalis L.and performance of potential biological actions.This paper has outlined 19 saponin monomers separated from Asparagus and summarized their anti-tumor functions and corresponding mechanism, which may serve as a reference for further researches.

  9. IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM

    Directory of Open Access Journals (Sweden)

    A.K.MoHD OMAR

    1999-01-01

    Full Text Available Artificially inoculated asparagus tissue culture plantlets with a virulent fungus, Fusarium proliferatum showed signs of infection as early as 4 days after inoculat ion. Macroscopic observations revealed presence of early symptoms such as necrotic lesions at the affected area and light microscopic examinations clearly revealed the post-penetration events that took place including the destruction of surrounding cells. However, little is known of the hyphal activity or advancement on the host's surface at the initial stage after inoculation. Scanning electron microscopic examination clearly revealed the hyphal advancement on the surface and the mode of entrance into the host tissues beneath. Four days after inoculation, the fungi proceeded to spread out from the inoculation point onto the host surface which eventually developed into a sparse network of both aerial and non-aerial hyphae. Non-aerial hyphae form a network of mycelium that adheres to the surface and it's movement appeared to be oriented towards the stomata. Hyphal penetration occurs more often through the stomata, natural openings or wounds. In some cases, the hyphae crossed over the stomatal opening w ithout entering the host tissues. At places where the cuticle layer is absent or not well developed the hyphae successfully grew in between the epidermal cells into the tissues beneath.

  10. Field experiments using the rhabditid nematode Phasmarhabditis hermaphrodita or salt as control measures against slugs in green asparagus

    NARCIS (Netherlands)

    Ester, A.; Rozen, van K.; Molendijk, L.P.G.

    2003-01-01

    Green asparagus (Asparagus officinalis L.) crops are frequently damaged by the grey field slug (Deroceras reticulatum). Most damage occurs in the soil, although some asparagus spears are attacked above ground. In the Netherlands damage occurs especially during the first 5 weeks of harvesting, from t

  11. Micropropagation of Asparagus by in vitro shoot culture.

    Science.gov (United States)

    Stajner, Nataša

    2013-01-01

    Asparagus officinalis is most extensively studied species within the genus Asparagus, which is well known as garden asparagus. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Male plants are high yielders and preferred commercially over female plants. Tissue culture techniques could efficiently promote vegetative propagation of male plants and pave the way for efficient plant breeding.This chapter describes an efficient micropropagation protocol for developing rapid growing in vitro Asparagus shoot cultures. The source of explants, inoculation, and shoot proliferation, followed by shoot propagation, rooting, and acclimatization is described. The optimal medium for Asparagus micropropagation described in this chapter is composed of MS macro- and microelements and a combination of auxins and cytokinins. Plant growth regulators NAA, kinetin, and BA were used in various concentrations. Three different media representing the whole micropropagation protocol of Asparagus are described; medium for shoot initiation, medium for shoot multiplication, and medium for root formation. By in vitro propagation of Asparagus, root initiation is difficult, but can be promoted by adding growth retardant ancymidol which also greatly promotes shoot development and suppresses callus formation.

  12. Micropropagation of Asparagus by in vitro shoot culture.

    Science.gov (United States)

    Stajner, Nataša

    2013-01-01

    Asparagus officinalis is most extensively studied species within the genus Asparagus, which is well known as garden asparagus. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Male plants are high yielders and preferred commercially over female plants. Tissue culture techniques could efficiently promote vegetative propagation of male plants and pave the way for efficient plant breeding.This chapter describes an efficient micropropagation protocol for developing rapid growing in vitro Asparagus shoot cultures. The source of explants, inoculation, and shoot proliferation, followed by shoot propagation, rooting, and acclimatization is described. The optimal medium for Asparagus micropropagation described in this chapter is composed of MS macro- and microelements and a combination of auxins and cytokinins. Plant growth regulators NAA, kinetin, and BA were used in various concentrations. Three different media representing the whole micropropagation protocol of Asparagus are described; medium for shoot initiation, medium for shoot multiplication, and medium for root formation. By in vitro propagation of Asparagus, root initiation is difficult, but can be promoted by adding growth retardant ancymidol which also greatly promotes shoot development and suppresses callus formation. PMID:23179712

  13. Optimization for Ultrasonic-assisted Extraction Technology of Polysaccharide from Asparagus officinalis Stem%超声波辅助提取芦笋茎中多糖工艺的优化

    Institute of Scientific and Technical Information of China (English)

    彭友舜; 宋士涛; 廉琪; 郑学芳; 黄云祥; 李汉臣

    2011-01-01

    [ Objective ] The aim was to optimize the ultrasonic-assisted extraction technology of polysaccharide from Asparagus officinalis stem. [ Method ] Polysaccharide was extracted from Asparagus officinalis stem by ultrasonic-assisted extraction method. Based on single-factor experiment, the ultrasonic-assisted extraction technology of polysaccharide from Asparagus officinalis stem was optimized by orthogonal experimental design. [ Result] The optimal extraction conditions of polysaccharide from Asparagus officinalis stem were as follows: the ratio of water to material was 20∶1; the extraction temperature was 85 ℃; and the extraction time was 60 min. Under the conditions, the yield of polysaccharide was up to 3. 64%. [ Conclusion ] The research provides reference for development and utilization of effective ingredients in Asparagus officinalis stem.%[目的]优化芦笋老茎中多糖的超声波辅助提取工艺.[方法]利用超声波辅助提取了芦笋老茎中的多糖,并在单因素试验的基础上采用正交试验设计时芦笋茎中多糖的提取工艺进行了优化.[结果]芦笋茎中多糖的最佳提取工艺条件为水料比20:1(ml/g)、提取温度85℃和提取时间60 min,该提取条件下芦笋多糖的提取率为3.64%.[结论]该研究为芦笋老茎中有效成分的开发利用提供了参考.

  14. Changes in Fusarium and arbuscular-mycorrhizal fungi communities as related with different asparagus cultural factors

    NARCIS (Netherlands)

    Yergeau, E.; Vujanovic, V.; St-Arnaud, M.

    2006-01-01

    Asparagus (Asparagus officinalis) is a high-value perennial vegetable crop that has shown a marked decline in productivity after many years of continuous harvesting. This decline is caused by an increase in both abiotic (autotoxicity, harvesting pressure) and biotic stresses [fungal infections, main

  15. Asparagus officinalis extract controls blood glucose by improving insulin secretion and β-cell function in streptozotocin-induced type 2 diabetic rats.

    Science.gov (United States)

    Hafizur, Rahman Md; Kabir, Nurul; Chishti, Sidra

    2012-11-14

    The aim of the present study was to evaluate the anti-diabetic mechanism of Asparagus officinalis, a dietary agent used for the management of diabetes. Streptozotocin (90 mg/kg) was injected in 2-d-old Wistar rat pups to induce non-obese type 2 diabetes. After confirmation of diabetes on the 13th week, diabetic rats were treated with a methanolic extract of A. officinalis seeds (250 and 500 mg/kg per d) or glibenclamide for 28 d. After the treatment, fasting blood glucose, serum insulin and total antioxidant status were measured. The pancreas was examined by haematoxylin-eosin staining and immunostained β- and α-cells were observed using a fluorescence microscope. Treatment of the diabetic rats with the A. officinalis extract at doses of 250 and 500 mg/kg suppressed the elevated blood glucose in a dose- and time-dependent manner. The 500 mg/kg, but not 250 mg/kg, dose significantly improved serum insulin levels in the diabetic rats. The insulin:glucose ratio was significantly increased at both doses in the A. officinalis-treated rats. Both qualitative and quantitative improvements in β-cell function were found in the islets of the A. officinalis-treated rats. The extract showed potent antioxidant activity in an in vitro assay and also improved the total antioxidant status in vivo. In most cases, the efficacy of A. officinalis (500 mg/kg) was very similar to a standard anti-diabetic drug, glibenclamide. Thus, the present study suggests that A. officinalis extract exerts anti-diabetic effects by improving insulin secretion and β-cell function, as well as the antioxidant status. PMID:22221560

  16. 芦笋不同组织水浸提液对辣椒的化感效应%Allelopathic Effect of Water Extracts from Different Parts of Asparagus officinalis L.on Hot Pepper

    Institute of Scientific and Technical Information of China (English)

    严泽生; 徐冬梅; 贺忠群

    2011-01-01

    [目的]测定芦笋不同组织浸提液对辣椒的化感效应.[方法]以辣椒为侵体,通过测定芦笋不同部分水浸提液对辣椒种子萌芽和幼苗生长的影响,研究芦笋对辣椒的化感效应.[结果]芦笋地上部和地下部组织水浸提液对辣椒种子发芽率、发芽指数均表现出抑制作用;高浓度芦笋地上部和地下部组织水浸提液对辣椒幼苗的生长及叶绿素含量均具有抑制作用,而低浓度浸提液具有促进作用.芦笋不同组织部位水浸提液的化感作用强弱不同,地上部分水浸提液化感作用明显强于地下部分.[结论]该研究为选择芦笋的间作作物及其秸杆的再利用提供了依据.%[ Objective ] To discuss the allelopathic effect of water extracts from different parts of Allelopathy of Asparagus officinalis L. On hot pepper. [Method] Using hot pepper as the receptor, effect of water extracts from different parts of Asparagus officinalis L. On seed germination and seedling growth were determined, investigating the allelopathy of Asparagus officinalis L. To hot pepper. [ Result ] Water extracts of aboveground and underground parts of Asparagus officinalis L. Showed inhibitory effect on germination rate and germination index of hot pepper. High concentration water extracts all showed inhibitory effect on seedling growth and content of chlorophyll,while low concentration ones presented facilitating effect on them. In addition, ailelopathic effect of water extracts from above ground of Asparagus officinalis L. Was stronger than that from under ground. [ Conclusion] This work is of considerable value for selection of interculture of Asparagus officinalis L. And reuse of straw of it.

  17. Determination and Analysis of Amino Acids in Different Parts of Asparagus officinalis L. Stem%芦笋茎不同部位氨基酸含量的分析测定

    Institute of Scientific and Technical Information of China (English)

    彭友舜; 王树元; 贾丹丹; 廉琪; 张红梅; 黄云祥

    2011-01-01

    [目的]测定分析芦笋茎不同部位中氨基酸含量,为芦笋营养价值的开发利用提供理论依据.[方法]利用氨基酸分析仪对芦笋茎不同部位氨基酸含量及组成进行测定和分析.[结果]18种氨基酸在不同部位均有检出,嫩尖中氨基酸总量、人体必需氨基酸含量、儿童必需氨基酸含量最高;嫩茎中鲜味氨基酸含量、支/芳比最高.[结论]芦笋茎不同部位具有不同的营养价值和药用价值,这为芦笋的开发利用提供了理论依据.%[ Objective ] To determine and analyze the contents of amino acids in different parts of Asparagus officinalis L. stem, so as to provide theoretical evidence for the development of nutrient value of Asparagus offtcinalis L.. [ Method] By employing amino acid analyzer, the contents of amino acids and their constituents were analyzed. [ Result] Eighteen amino acids were analyzed in each part of Asparagus officinalis L. stem. The contents of amino acids, essential amino acids for mankind and children in tender stem tip were the highest, whereas the content of delicious amino acid and branched chain amino acid to aromatic amino acid ratio in tender stem were the highest. [ Conclusion ] Different parts of Asparagus officinalis L. stem are endowed with different nutrient and medicinal value, which provides evidence for the development of Asparagus offinalis L..

  18. 芦笋老茎总皂甙提取工艺优化%Optimization of coarse saponins extraction from the older stems of asparagus officinalis

    Institute of Scientific and Technical Information of China (English)

    蔡金星; 刘秀凤; 常学东; 冯清爱; 张兴运

    2009-01-01

    以芦笋老茎为原料,研究提取温度、提取时间、乙醇浓度、料液比对芦笋皂甙提取的影响.通过Taguchi稳健试验设计,确定最佳提取工艺条件为:提取温度75℃,提取时间2.5 h,乙醇体积浓度90%,料液比1:15(W:V),提取次数为2次.%The extraction of coarse saponins from the older stems of Asparagus Officinalis was optimized by employing Taguehi Robust Experimental Design. The optimum extraction conditions were as follows: concentration of ethanol,90% (V : V) ; temperature,75 ℃; ratio of material to ethanol solution, 1 : 15(W : V) ; extraction time,2. 5 h; and the extraction was executed twice.

  19. An observational study and quantification of the actives in a supplement with Sambucus nigra and Asparagus officinalis used for weight reduction.

    Science.gov (United States)

    Chrubasik, Cosima; Maier, Thorsten; Dawid, Corinna; Torda, Thomas; Schieber, Andreas; Hofmann, Thomas; Chrubasik, Sigrun

    2008-07-01

    The aim of the study was to obtain information on the content of co-active compounds of a food supplement recommended as a weight reduction diet and on its short-term effectiveness and safety as a starter for lifestyle change. Eighty participants completed the protocol. The Sambucus nigra L. berry juice enriched with flower extract and tablets containing berry powder and flower extract provided a total of 1 mg anthocyanins, 370 mg flavonol glycosides and 150 mg hydroxycinnamates per day; the Asparagus officinalis L. powder tablets provided 19 mg saponins per day. After the diet, the mean weight, blood pressure, physical and emotional well-being and the quality of life had significantly improved (ITT analysis). The effectiveness and tolerability of the regimen were rated as very good or good by most of the completers. It remains to be established if any particular compounds contribute to the efficacy of the diet. PMID:18350516

  20. Sugar regulation of harvest-related genes in asparagus.

    Science.gov (United States)

    Davies, K M; Seelye, J F; Irving, D E; Borst, W M; Hurst, P L; King, G A

    1996-07-01

    The signals controlling the abundance of transcripts up-regulated (pTIP27, pTIP31, and pTIP32) or down-regulated (pTIP20 and pTIP21) after harvest in asparagus (Asparagus officinalis L.) spears were examined. pTIP27 and pTIP31 are known to encode asparagine synthetase (AS) and a beta-galactosidase (beta-gal) homolog, respectively. The nucleotide sequences of pTIP20, pTIP21, and pTIP32 were determined, and they encode histone 3, histone 2B, and an unknown product, respectively. Changes in respiration, soluble sugars, and abundance of the five mRNAs were similar in the tips stored as 30-mm lengths or as part of 180-mm spears. We previously hypothesized that sugars may regulate the level of AS transcripts in asparagus tissue. Asparagus cell cultures were used to test the role of sugar status may regulate the level of AS transcripts in asparagus tissue. Asparagus cell cultures were used to test the role of sugar status in regulating gene expression. Transcript abundance for AS, beta-gal, and pTIP32 was low in cells in sugar-containing medium but increased within 12 h after transferring cells to a sugar-free medium. Histone 3 and histone 2B transcripts were, in general, abundant in cells on sugar-containing medium but declined in abundance when transferred to sugar-free medium. When cells were returned to sugar-containing medium the abundance of transcripts for histone 3 and histone 2B increased, whereas that for AS, beta-gal, and pTIP32 decreased. Soluble sugar levels are known to decline rapidly in the tips of harvested spears. Metabolic regulation by sugar status may have a major influence on gene expression in asparagus spears and other tissue after harvest. PMID:8754687

  1. 不同灌水下限对沙培芦笋生长生理指标的影响%Effects of Irrigation Threshold on Growth and Physiological Indexes of Asparagus(Asparagus officinalis L.) in Sand

    Institute of Scientific and Technical Information of China (English)

    裴红霞; 杨海玲; 赵云霞; 颜秀娟; 高晶霞; 秦小军

    2013-01-01

    [目的]确定合理的芦笋(Asparagus officinalisL.)灌水控制指标.[方法]采用盆栽试验,设置3个灌水下限(80%~ 85 %fc、65%~70%fc、45%~50%fc),研究不同灌水下限对沙培芦笋生长生理指标的影响.[结果]灌水下限对芦笋生长状况和生理指标均有显著影响..45% ~ 50%fc灌水下限条件下植株生长发育良好,根数多,主根长;一定范围内,MDA含量和SOD、POD、CAT活性随着灌水下限的提高明显增加,说明较高的灌水下限可能一定程度上对芦笋形成了胁迫,芦笋可通过提高体内的抗氧化酶活性来减轻由此带来的伤害.因此,选择土壤相对含水率45%~50%fc作为沙培芦笋的灌水下限指标.[结论]不同灌水下限是影响芦笋生长生理指标的主要因素.该研究可为沙培芦笋生产提供科学依据.

  2. Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization.

    Science.gov (United States)

    Deng, Chuan-liang; Wang, Ning-na; Li, Shu-fen; Dong, Tian-yu; Zhao, Xin-peng; Wang, Shao-jing; Gao, Wu-jun; Lu, Long-dou

    2015-09-01

    Garden asparagus (Asparagus officinalis L.) is a dioecious species whose male and female flowers are found in separate unisexual individuals. A region called the M-locus, located on a pair of homomorphic sex chromosomes, controls sexual dimorphism in asparagus. To date, no sex determining gene has been isolated from asparagus. To identify more genes involved in flower development in asparagus, subtractive hybridization library of male flowers in asparagus was constructed by suppression subtraction hybridization. A total of 107 expressed sequence tags (ESTs) were identified. BLASTX analysis showed that the library contained several genes that could be related to flower development. The expression patterns of seven selected genes believed to be involved in the development of asparagus male flower were further analyzed by semi-quantitative or real-time reverse-transcription polymerase chain reaction (RT-PCR). Results showed that AOEST4-5, AOEST12-40, and AOEST13-38 were strongly expressed in the male flower stage, whereas no transcript level of AOEST13-38 was detected in the female flower stage. The expression levels of AOEST13-87, AOEST13-92, AOEST13-40, and AOEST18-87 in the male flower stage were also higher than those in the female flower stage, although these transcripts were also expressed in other tissues. The identified genes can provide a strong starting point for further studies on the underlying molecular differences between the male and female flowers of asparagus.

  3. Hypocholesterolemic and hepatoprotective effects of "triguero" asparagus from andalusia in rats fed a high cholesterol diet.

    Science.gov (United States)

    García, M D; De la Puerta, R; Sáenz, M T; Marquez-Martín, A; Fernández-Arche, M A

    2012-01-01

    The cultivated species of the wild autochthonous Asparagus officinalis in Andalusia in Spain is commonly called "triguero" asparagus. This vegetable has traditionally been very much appreciated for its organoleptic and nutritional characteristics. This study has been designed to evaluate the potential effect of different concentrations of freeze-dried asparagus (500, 250, and 125 mg/Kg of body weight/day) on oxidative status and lipid profile in rats fed a cholesterol-rich diet. After five weeks of treatment, doses of 250 and 500 mg/Kg of asparagus were able to significantly reduce total cholesterol and LDL cholesterol levels. Atherogenic index was also significantly reduced in a dose-dependent manner by administrating freeze-dried asparagus. A beneficial effect was observed in the HDL cholesterol levels in asparagus-fed groups although the increase was not significant. Consumption of asparagus also improved antioxidant status, assayed superoxide dismutase (SOD) and catalase (CAT) enzymes, and protected against lipid peroxidation. These results show that the intake of green asparagus from Andalusia (Spain) helps to regulate plasma lipid levels and prevents oxidative damage in hypercholesterolemic conditions. PMID:22203881

  4. Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization.

    Science.gov (United States)

    Deng, Chuan-liang; Wang, Ning-na; Li, Shu-fen; Dong, Tian-yu; Zhao, Xin-peng; Wang, Shao-jing; Gao, Wu-jun; Lu, Long-dou

    2015-09-01

    Garden asparagus (Asparagus officinalis L.) is a dioecious species whose male and female flowers are found in separate unisexual individuals. A region called the M-locus, located on a pair of homomorphic sex chromosomes, controls sexual dimorphism in asparagus. To date, no sex determining gene has been isolated from asparagus. To identify more genes involved in flower development in asparagus, subtractive hybridization library of male flowers in asparagus was constructed by suppression subtraction hybridization. A total of 107 expressed sequence tags (ESTs) were identified. BLASTX analysis showed that the library contained several genes that could be related to flower development. The expression patterns of seven selected genes believed to be involved in the development of asparagus male flower were further analyzed by semi-quantitative or real-time reverse-transcription polymerase chain reaction (RT-PCR). Results showed that AOEST4-5, AOEST12-40, and AOEST13-38 were strongly expressed in the male flower stage, whereas no transcript level of AOEST13-38 was detected in the female flower stage. The expression levels of AOEST13-87, AOEST13-92, AOEST13-40, and AOEST18-87 in the male flower stage were also higher than those in the female flower stage, although these transcripts were also expressed in other tissues. The identified genes can provide a strong starting point for further studies on the underlying molecular differences between the male and female flowers of asparagus. PMID:26038270

  5. 芦笋下脚料皂苷超声提取工艺%Ultrasonic-assisted Extraction of Saponins from Asparagus officinalis Scraps

    Institute of Scientific and Technical Information of China (English)

    孙健; 李曼; 王丽卫; 赵兵

    2011-01-01

    In order to improve the utilization rate of Asparagus officinalis L. scraps, ultrasonic assistance was applied for the extraction of saponins from the materials. After two rounds of uniform design, the optimal extraction conditions for achieving maximum extraction rate of saponins were material-to-liquid ratio of 1:1 (m/V), extraction temperature of 30 ℃, duty cycle of 0.4:1.6 (ratio of occupied to empty space), ultrasonic power of 200 W, extraction time of 30 min, extraction pH of 5.05 and ethanol concentration of 70%. Under the optimal conditions, the extraction rate of saponins was up to 7.5%, and the recovery rate was 98.78% after three rounds of extraction. The saponins could be fully recovered by three rounds of extractions in a 2 L circulated ultrasonic-assisted extraction device. Therefore, ultrasonic-assisted extraction is suitable for the extraction of saponins from Asparagus officinalis L. scraps.%采用超声强化技术对芦笋下脚料中的皂苷成分进行提取,通过两轮均匀试验对提取工艺进行优化。结果得到的最优提取工艺为液料比1:1、温度30℃、占空比0.4:1.6、功率200W、提取时间30min、pH5.05、酒精体积分数70%,该工艺条件下单次提取得率为7.5%(干基);两次提取累积回收率达到93.37%,3次提取累积回收率达到98.40%。在2L循环超声提取装置中的放大实验表明,两次提取累积回收率达到97.49%,3次可提取完全。表明超声强化方法适用于芦笋下脚料中皂苷的提取。

  6. Saponin profile of green asparagus genotypes.

    Science.gov (United States)

    Vázquez-Castilla, Sara; Jaramillo-Carmona, Sara; Fuentes-Alventosa, Jose María; Jiménez-Araujo, Ana; Rodríguez-Arcos, Rocío; Cermeño-Sacristán, Pedro; Espejo-Calvo, Juan Antonio; Guillén-Bejarano, Rafael

    2013-11-20

    The main goal of this study was to determine the saponin profiles of different "triguero" asparagus genotypes and to compare them to green asparagus commercial hybrids. The samples consisted of 31 commercial hybrids and 58 genotypes from the Huétor-Tájar (HT) population variety ("triguero"). The saponin analysis by high-performance liquid chromatography-mass spectrometry allowed for the determination of 12 saponins derived from a furostan-type steroidal genin, 4 of which had never been described in the edible part of asparagus. The saponin profile of "triguero" asparagus was a combination of these new saponins and protodioscin. Although protodioscin was the major saponin found in commercial hybrids, some of these 12 saponins were detected as major components in some of the commercial hybrids. The total contents of saponins described in some of these HT genotypes reach values as high as 10-100 times higher than those found in commercial hybrids.

  7. Flavonoid profile of green asparagus genotypes.

    Science.gov (United States)

    Fuentes-Alventosa, J M; Jaramillo, S; Rodríguez-Gutiérrez, G; Cermeño, P; Espejo, J A; Jiménez-Araujo, A; Guillén-Bejarano, R; Fernández-Bolaños, J; Rodríguez-Arcos, R

    2008-08-27

    The determination of flavonoid profiles from different genotypes of triguero asparagus and their comparison to those from green asparagus commercial hybrids was the main goal of this study. The samples consisted of 32 commercial hybrids and 65 genotypes from the Huetor-Tajar population variety (triguero). The analysis of individual flavonoids by HPLC-DAD-MS has allowed the determination of eight naturally occurring flavonol derivatives in several genotypes of triguero asparagus. Those compounds included mono-, di-, and triglycosides of three flavonols, that is, quercetin, isorhamnetin, and kaempferol. The detailed analysis of the flavonoid profiles revealed significant differences among the distinct genotypes. These have been classified in three distinct groups as the result of a k-means clustering analysis, two of them containing both commercial hybrids and triguero asparagus and another cluster constituted by 21 genotypes of triguero asparagus, which contain several key flavonol derivatives able to differentiate them. Hence, the triglycosides tentatively identified as quercetin-3-rhamnosyl-rutinoside, isorhamnetin-3-rhamnosyl-rutinoside, and isorhamnetin-3-O-glucoside have been detected only in the genotypes grouped in the above-mentioned cluster. On the other hand, the compound tentatively identified as isorhamnetin-3-glucosyl-rutinoside was present in most genotypes of triguero asparagus, whereas it has not been detected in any of the commercial hybrids.

  8. Comparative sequence and genetic analyses of asparagus BACs reveal no microsynteny with onion or rice.

    Science.gov (United States)

    Jakse, Jernej; Telgmann, Alexa; Jung, Christian; Khar, Anil; Melgar, Sergio; Cheung, Foo; Town, Christopher D; Havey, Michael J

    2006-12-01

    The Poales (includes the grasses) and Asparagales [includes onion (Allium cepa L.) and asparagus (Asparagus officinalis L.)] are the two most economically important monocot orders. The Poales are a member of the commelinoid monocots, a group of orders sister to the Asparagales. Comparative genomic analyses have revealed a high degree of synteny among the grasses; however, it is not known if this synteny extends to other major monocot groups such as the Asparagales. Although we previously reported no evidence for synteny at the recombinational level between onion and rice, microsynteny may exist across shorter genomic regions in the grasses and Asparagales. We sequenced nine asparagus BACs to reveal physically linked genic-like sequences and determined their most similar positions in the onion and rice genomes. Four of the asparagus BACs were selected using molecular markers tightly linked to the sex-determining M locus on chromosome 5 of asparagus. These BACs possessed only two putative coding regions and had long tracts of degenerated retroviral elements and transposons. Five asparagus BACs were selected after hybridization of three onion cDNAs that mapped to three different onion chromosomes. Genic-like sequences that were physically linked on the cDNA-selected BACs or genetically linked on the M-linked BACs showed significant similarities (e asparagus and rice across these regions. Genic-like sequences that were linked in asparagus were used to identify highly similar (e asparagus and genetic linkages in onion. These results further indicate that synteny among grass genomes does not extend to a sister order in the monocots and that asparagus may not be an appropriate smaller genome model for plants in the Asparagales with enormous nuclear genomes. PMID:17016688

  9. Cold-resistance seedling cultivation technology of Asparagus officinalis in Heilongjiang%黑龙江省芦笋育苗防冻技术

    Institute of Scientific and Technical Information of China (English)

    赵凤臣; 吴洪军; 于春海; 王玉莲

    2003-01-01

    @@ 芦笋,学名石刁柏(Asparagus officinalis L.)百合科天门冬属,其嫩茎味道鲜美,是世界十大名菜之一,长期食用可治疗多种疾病,对肺、肝、乳腺等多种癌症具有防治作用,是国内外市场畅销菜.近年来人工栽培业发展十分迅速,目前黑龙江省引种栽培已取得成功,但由于气候寒冷,生长期短,1年生苗常因木质化程度不够而遭受冻害,造成烂根烂芽,严重影响笋苗的产量和质量,几年来通过反复试验,我们总结出了一些芦笋育苗防冻措施,生产应用收效较好,现将其介绍如下.

  10. Structural Changes in Asparagus Production and Exports from Peru

    OpenAIRE

    Shimizu, Tatsuya

    2009-01-01

    Asparagus is the star product among non-traditional agricultural exports (NTAXs) in Peru. The export of preserved asparagus has expanded since the end of the 1980s. Although there was some stagnation in the mid-1990s, exports of fresh asparagus have expanded rapidly since the end of the 1990s. Now, the export of both preserved and fresh asparagus constitute the second most important agricultural export in Peru after coffee. Besides the change in demand on the international market, the importa...

  11. 芦笋单倍体染色体加倍技术研究%Research on the Technique of Chromosome Doubling of Haploids of Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张天翔; 林宗铿; 蔡坤秀; 杨俊杰

    2011-01-01

    The technique of chromosome doubling of haploids of Asparagus officinalis L. have been studied in this paper. In vitro chromosome doubling of haploids was studied by soaking shoot tips in colchicine solution and adding colchicine in the culture medium. The results indicated that adding colchicine in the culture medium increased the efficiency of chromosome doubling than that by soaking method. The induction rate and the survival rate of treating A. officinalis with 0.3% colchicine for 7 days by adding colchicine in the culture medium reached 82.50% and 80%. Compared with the haploid, the diploid stems showed thinker with larger guard cell and stoma and more chloroplasts.%研究了芦笋单倍体染色体加倍的方法.在离体培养条件下,以秋水仙素为诱变剂,分别用浸泡法和培养基添加法处理芦笋单倍体幼苗的茎尖,比较了秋水仙素不同浓度、不同处理时间的诱导效果.结果表明,培养基添加法的诱导效果好于浸泡法,当在培养基中添加0.3%秋水仙素并处理7天时诱导效果最佳,染色体加倍频率与成活率分别可达82.50%和80%.加倍后的二倍体植株与单倍体植株相比,茎干变粗,气孔与保卫细胞增大,保卫细胞内的叶绿体数增多.

  12. 绿芦笋粉挥发油提取工艺的优化及成分分析%Optimization of Extraction Process of Essential Oil from GreenAsparagus officinalis. Powder and Its Components Analysis

    Institute of Scientific and Technical Information of China (English)

    康旭; 袁江兰; 孟鸳; 邓川

    2011-01-01

    [ Objective] To optimize the extraction process of volatile oil from green Asparagus officinalis L. powder and analyze its components, so as to provide basis for the further research and comprehensive application of green Asparagus officinalis L. powder. [ Method ] The extraction process of supercritical CO2 extraction was optimized by four factors and three levels orthogonal test with extraction content of volatile oil as evaluation index, and the chemical compositions of the extraction were analyzed by GC-MS. [ Result] The optimal extraction conditions of supercritical CO2 extraction for essential oil from green Asparagus officinalis L. powder were determined to be CO2 flow rate of 20L/h, extraction temperature of 35 ℃ , extraction pressure of 25 MPa and extraction duration of 2h, under which the extraction rate of volatile oil from green Asparagus officinalis L. powder was up to 98%. Thirty-seven components were isolated and obtained from the volatile oil, of which contents of hydrocarbons and aldehydes and esters ranked the first and second respectively. [ Conclusion ] The extraction rate of essential oil from green Asparagus officinalis L. powder by SC-CO2 is relative higher under the optimum extraction conditions, which provides basis for the further research and comprehensive application of green Asparagus officinalis L. powder.%[目的]优化超临界CO2流体萃取技术(SC-CO2)提取绿芦笋粉挥发油的工艺,并对其化学成分进行分析,为绿芦笋粉的深入研究和综合应用奠定基础.[方法]以挥发油提取量为评价指标,设计4因素3水平正交试验优化超临界CO2萃取绿芦笋粉挥发油的工艺条件,利用GC-MS对萃取物化学成分进行分析.[结果]超临界CO2萃取绿芦笋粉挥发油的最佳条件为:CO2流量20 L/h,萃取温度35 ℃,萃取压力25 MPa,萃取时间2 h,在此条件下,绿芦笋粉挥发油的提取率达到98%;主要包括37种化合物,其中以烷类含量最高,其次是醛类和

  13. Asparagus racemosus--an update.

    Directory of Open Access Journals (Sweden)

    Goyal R

    2003-09-01

    Full Text Available Asparagus racemosus (Shatavari is recommended in Ayurvedic texts for prevention and treatment of gastric ulcers, dyspepsia and as a galactogogue. A. racemosus has also been used successfully by some Ayurvedic practitioners for nervous disorders, inflammation, liver diseases and certain infectious diseases. However, no scientific proof justifying aforementioned uses of root extract of A. racemosus is available so far. Recently few reports are available demonstrating beneficial effects of alcoholic and water extracts of the root of A. racemosus in some clinical conditions and experimentally induced diseases, e.g. galactogogue effect, antihepatotoxic and immunomodulatory activities. The present article includes the detailed exploration of pharmacological properties of the root extract of A. racemosus reported so far.

  14. Food idiosyncrasies: beetroot and asparagus.

    Science.gov (United States)

    Mitchell, S C

    2001-04-01

    Anecdotal observations scattered throughout the literature have often provided clues to underlying variations in humans' ability to handle dietary chemicals. Beetroot, the red root of the garden beet used extensively as a food source, is known to produce red urine in some people following its ingestion, whereas others appear to be able to eat the vegetable with impunity. Asparagus, a vegetable whose young shoots have been eaten as a delicacy since the times of the Roman Empire, has been associated with the production of a malodorous urine smelling like rotten cabbage. Those who produce this odor assume that everyone does, and those who do not produce it have no idea of its potential olfactory consequences. These two examples, where the population appears divided in its ability to process food products or more precisely the chemicals contained within them, are reviewed in detail in this article. PMID:11259347

  15. Increase the foliage area of Asparagus Officinalis L. Cv. UC 157 F1 “asparagus” by the spraying of Gibberellin (AG3 and 6 – Benzilaminopurine (6 – BAP

    Directory of Open Access Journals (Sweden)

    José Paraguay Mercado

    2010-12-01

    Full Text Available The present work had as objective to study the increment of the leaf area of Asparagus Officinalis L., for they were used it asparagus crown with 5 yolks, to those that were applied by aspersion, different concentrations of the hormones of gibberellic acid and 6-bencilaminopurina which were applied at the 25, 60, 110 and 160 days respectively. It was found that in the different rehearsed treatments one doesn't observe differential significant in the increment of the number of plants, plant height, number of yolks and dry weight of foliage. However it was observed that the combined application of the phytohormonas in the range of 0.110 at 0.230 m of gibberllic acid and 0.037 to 0.075 m of 6-Bencilaminopurin a bigger number of yolk is achieved (13%, sprout (7%, plant height (11.3% and dry weight (7.3% in the treated plants. Concluded to continue making studies of sinergism of these two phytohormonas, gibberellic (AG3 and 6-bencilaminopurina (6-BAP, seeking to achieve adequate levels of leaf area increase of the leaf area in the asparagus.

  16. 芦笋及近缘种重要活性因子与主要功能研究进展%Research progress in key active factors and major function on Asparagus officinalis L.and its wild relatives

    Institute of Scientific and Technical Information of China (English)

    张岳平; 瞿华香; 谢启鑫; 赵萍; 陈光宇

    2013-01-01

    Asparagus officinalis L. is an important high nutrition vegetable rich in bioactive factors such as rutin, saponins,plant polysaccharides,organic selenium,free amino acid and vitamins. It is also considered as one of important herbal medicine plants which can prevent and treat several diseases for human such as tumour, hypertension,hyperlipidemia,hyperglycemia and obesity. The latest advances in the areas of the bioactive factors,their major functions,and pharmacological mechanism in Asparagus officinalis L. and its wild relatives were reviewed. The prospect of Asparagus officinalis L as an important functional vegetable both of food and medicine for the future was made as well.%芦笋是重要的富含芦丁、皂甙、植物多糖、有机硒、游离氨基酸和维生素等多种生物活性因子的营养型蔬菜,也是能预防和治疗肿瘤、高血压、高血脂、高血糖和肥胖等多种疾病的功能型药用植物 本文综述了芦笋及近缘种的重要活性因子类型、主要功能及其作用机制的最新研究进展,并展望了芦笋作为功能型药食兼用蔬菜的发展趋势.

  17. Identification of Marchfeld asparagus using Sr isotope ratio measurements by MC-ICP-MS.

    Science.gov (United States)

    Swoboda, S; Brunner, M; Boulyga, S F; Galler, P; Horacek, M; Prohaska, T

    2008-01-01

    This work focuses on testing and application of Sr isotope signatures for the fast and reliable authentication and traceability of Asparagus officinalis originating from Marchfeld, Austria, using multicollector inductively coupled plasma mass spectrometry after optimised Rb/Sr separation. The major sample pool comprises freeze-dried and microwave-digested asparagus samples from Hungary and Slovakia which are compared with Austrian asparagus originating from the Marchfeld region, which is a protected geographical indication. Additional samples from Peru, The Netherlands and Germany were limited in number and allowed therefore only restricted statistical evaluation. Asparagus samples from Marchfeld were harvested within two subsequent years in order to investigate the annual variation. The results show that the Sr isotope ratio is consistent within these 2 years of investigation. Moreover, the Sr isotope ratio of total Sr in soil was found to be significantly higher than in an NH4NO3 extract, reflecting the mobile (bioavailable) phase. The isotope composition in the latter extract corresponds well to the range found in the asparagus samples in Marchfeld, even though the concentration of Sr in asparagus shows no direct correlation to the concentration of Sr in the mobile phase of the soil. The major question was whether the 'Marchfelder Spargel' can be distinguished from samples from the neighbouring countries of Hungary and Slovakia. According to our findings, they can be clearly (100%) singled out from the Hungarian samples and can be distinguished from the Slovakian asparagus samples with a probability of more than 80%. PMID:17874075

  18. Copitarsia decolora (Lepidoptera: Noctuidae) larvae escaping from discarded asparagus: data in support of a pathway risk analysis.

    Science.gov (United States)

    Gould, J R; Maldonado, M Huamán

    2006-10-01

    This research was undertaken to gather data in support of an assessment of the likelihood that Copitarsia decolora (Guenée) (Lepidoptera: Noctuidae), a pest of asparagus, Asparagus officinalis L., and other crops, could escape from the pathway followed by asparagus from the field to the consumer. Asparagus that is destroyed by cooking and consumption, being run through a trash compactor or garbage disposal, or being buried in a landfill probably cannot support development of C. decolora larvae. Much asparagus is discarded in dumpsters, however, and the time between disposal and removal to the landfill provides an opportunity for C. decolora to escape into the environment. Results of this study indicate that C. decolora cannot survive to the pupal stage on rotten asparagus, and survival on dried asparagus is low. However, larvae can survive at least 1 wk on both types of deteriorating asparagus held at 23.5 degrees C. In field trials, a small percentage of C. decolora larvae crawled out of a dumpster filled with asparagus after 1 wk. PMID:17066789

  19. A Preliminary Study on Isolated Microspore Culture of Asparagus officinalis%芦笋游离小孢子培养研究初报

    Institute of Scientific and Technical Information of China (English)

    汤泳萍; 周劲松; 罗绍春; 盛文涛; 谢启鑫; 朱友林; 陈光宇

    2011-01-01

    In this paper, the isolated microspore culture is studied with five cultivars or hybrid combinations of Asparagus officinalis. The results show that the optimal time for the inducement of callus is near the monokaryotic stage; there is significant difference in the inducement rate of callus among different genotypes; the frequency of the inflated microspore treated by different temperatures is 6 times higher than that of the control, and the highest frequency reaches 22.5%; the suitable medium for the isolated microspore culture is MS + 0.5 mg/L 6 - BA + 1.0 mg/L NAA + 0.2 mg/L 2,4 - D + 400 mg/L Glu + 6% sucrose.%以5个芦笋品种不同发育时期的花药为试验材料,进行芦笋游离小孢子培养,探索高频率获得雄核发育个体的技术途径,为芦笋全雄育种和杂种优势利用服务.初步试验结果表明:单核靠边期是小孢子适宜培养的最佳时期:不同的基因型之间小孢子愈伤组织诱导率差异显著;对花药小孢子进行变温预处理,其膨大率比对照高出6倍多,最高达到22.5%;小孢子培养的适宜培养基为Ms加0.5 mg/L 6-BA、1.0 mg/L NAA、0.2 mg/L 2,4-D.400 ms/L Glu和6%的蔗糖.

  20. Purification of total saponins from Asparagus officinalis by macroporous resin%大孔树脂纯化芦笋总皂苷的工艺研究

    Institute of Scientific and Technical Information of China (English)

    张若洁; 徐永霞; 王鲁峰; 陈清婵; 徐晓云; 潘思轶; 王可兴

    2012-01-01

    目的 研究芦笋总皂苷的大孔树脂纯化工艺.方法 采用静态及动态吸附解吸试验优选适宜的大孔树脂,并优化纯化条件.结果 S-8型树脂对芦笋总皂苷有较好的吸附和洗脱效果,并具有良好的脱色效果,其最佳纯化条件为上样质量浓度1 mg/mL,上样量60 mL/g树脂,用pH 7的75%乙醇冼脱,洗脱剂用量为10 mL/g树脂.纯化后得提取物中芦笋总皂苷的质量分数可达50.15%,脱色率为92.23%.结论 S-8型大孔树脂用于富集芦笋总皂苷效果最佳,是一种理想的分离纯化介质.%Objective To investigate the purification of saponins from Asparagus officinalis by macroporous resin. Methods Macroporous resin was chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. Results S-8 macroporous resin was found to have good adsorption and desorption effects and show an excellent decolor effect. The optimum purification conditions were 1 mg/mL of saponins eluted by 75% ethanol at pH value 7 with a volume of 10 mL/g, and the loaded amount was 60 mL/g. The purity of total saponons increased to 50.15% after the purification, and the decoloration rate maintained 92.23%. Conclusion S-8 is an ideal resin with the best enrichment for separating and purifying total saponins from A. officinalis.

  1. Characterization of Cell Wall Components and Their Modifications during Postharvest Storage of Asparagus officinalis L.: Storage-Related Changes in Dietary Fiber Composition.

    Science.gov (United States)

    Schäfer, Judith; Wagner, Steffen; Trierweiler, Bernhard; Bunzel, Mirko

    2016-01-20

    Changes in cell wall composition during storage of plant foods potentially alter the physiological effects of dietary fiber components. To investigate postharvest cell wall modifications of asparagus and their consequences in terms of insoluble dietary fiber structures, asparagus was stored at 20 and 1 °C for different periods of time. Structural analyses demonstrated postharvest changes in the polysaccharide profile, dominated by decreased portions of galactans. Increasing lignin contents correlated with compositional changes (monolignol ratios and linkage types) of the lignin polymer as demonstrated by chemical and two-dimensional nuclear magnetic resonance (2D-NMR) methods. Depending on the storage time and temperature, syringyl units were preferentially incorporated into the lignin polymer. Furthermore, a drastic increase in the level of ester-linked phenolic monomers (i.e., p-coumaric acid and ferulic acid) and polymer cross-links (di- and triferulic acids) was detected. The attachment of p-coumaric acid to lignin was demonstrated by 2D-NMR experiments. Potential consequences of postharvest modifications on physiological effects of asparagus dietary fiber are discussed.

  2. Characterization of Cell Wall Components and Their Modifications during Postharvest Storage of Asparagus officinalis L.: Storage-Related Changes in Dietary Fiber Composition.

    Science.gov (United States)

    Schäfer, Judith; Wagner, Steffen; Trierweiler, Bernhard; Bunzel, Mirko

    2016-01-20

    Changes in cell wall composition during storage of plant foods potentially alter the physiological effects of dietary fiber components. To investigate postharvest cell wall modifications of asparagus and their consequences in terms of insoluble dietary fiber structures, asparagus was stored at 20 and 1 °C for different periods of time. Structural analyses demonstrated postharvest changes in the polysaccharide profile, dominated by decreased portions of galactans. Increasing lignin contents correlated with compositional changes (monolignol ratios and linkage types) of the lignin polymer as demonstrated by chemical and two-dimensional nuclear magnetic resonance (2D-NMR) methods. Depending on the storage time and temperature, syringyl units were preferentially incorporated into the lignin polymer. Furthermore, a drastic increase in the level of ester-linked phenolic monomers (i.e., p-coumaric acid and ferulic acid) and polymer cross-links (di- and triferulic acids) was detected. The attachment of p-coumaric acid to lignin was demonstrated by 2D-NMR experiments. Potential consequences of postharvest modifications on physiological effects of asparagus dietary fiber are discussed. PMID:26671648

  3. Determination of Fatty Acid in Asparagus by Gas Chromatography

    Directory of Open Access Journals (Sweden)

    Zehra HAJRULAI-MUSLIU

    2016-05-01

    Full Text Available Asparagus contain a lot of macronutrients and micronutrients including folate, dietary fibre (soluble and insoluble and phenolic compounds. Also asparagus is a good source of unsaturated linoleic and linolenic fatty acids which are precursors for Eicosapentanoic acid (EPA and Docosahexanoic acid (DHA. Unsaturated fatty acids have important biological effects and they have important role in human health. The objective of this study was to analyze fatty acid composition of asparagus as a potential source of linoleic and linolenic acid - a precursor for EPA and DHA. For this reason we analyzed fifty seven samples of asparagus collected from the local market. We used AOAC 996.06 method and analyses were performed with gas chromatograph with flame-ionization detector (GC-FID. The highest concentration of fatty acid in the asparagus was linoleic acid (C18:2n6 which content in asparagus is 25.620±1.0%. Also, asparagus is good source of -linolenic fatty acid (C18:3n3 and content of this fatty acid in asparagus is 8.840±0.3%. The omega-6 to omega-3 (n6/n3 ratio in asparagus was 3.19. Polyunsaturated fatty acids (PUFAs were higher than monounsaturated fatty acids (MUFAs, and from saturated fatty acids, palmitic acid was most frequent with 24.324±1.0%. From our study we can conclude that asparagus is very good source of unsaturated fatty acids, especially linoleic and linolenic fatty acids.

  4. Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus.

    Science.gov (United States)

    Sunada, Kayano; Ding, Xin Geng; Utami, Melia Sandya; Kawashima, Yoko; Miyama, Yoko; Hashimoto, Kazuhito

    2008-06-25

    TiO 2 photocatalytic decomposition and detoxification of phytotoxic compounds released by the roots of asparagus ( Asparagus officinalis L.) were investigated from the viewpoint of conservation-oriented cultivation. The phytotoxically active fraction was extracted either from dried asparagus roots or from the recycled nutrient solution of an asparagus hydroponic cultivation system. We found that the phytotoxic activity gradually decreased in the fraction with TiO 2 powder under irradiation with ultraviolet (UV) light at an intensity of 1.0 mW/cm (2). The growth of asparagus plants under actual cultivation conditions was also investigated by comparing asparagus grown in a hydroponic system where recycled waste nutrient solution was photocatalytically treated with solar light and a system with untreated recycled waste nutrient solution. The results showed, as measured by growth indices such as stem length and stem thickness, that asparagus growth in the photocatalytically treated system was superior to the untreated one. Furthermore, the yield of asparagus spears was 1.6-fold greater in the photocatalytically treated system, demonstrating the detoxification effect on the phytotoxic compounds and also the killing effect on pathogenic microorganisms.

  5. Detoxification of phytotoxic compounds by TiO2 photocatalysis in a recycling hydroponic cultivation system of asparagus.

    Science.gov (United States)

    Sunada, Kayano; Ding, Xin Geng; Utami, Melia Sandya; Kawashima, Yoko; Miyama, Yoko; Hashimoto, Kazuhito

    2008-06-25

    TiO 2 photocatalytic decomposition and detoxification of phytotoxic compounds released by the roots of asparagus ( Asparagus officinalis L.) were investigated from the viewpoint of conservation-oriented cultivation. The phytotoxically active fraction was extracted either from dried asparagus roots or from the recycled nutrient solution of an asparagus hydroponic cultivation system. We found that the phytotoxic activity gradually decreased in the fraction with TiO 2 powder under irradiation with ultraviolet (UV) light at an intensity of 1.0 mW/cm (2). The growth of asparagus plants under actual cultivation conditions was also investigated by comparing asparagus grown in a hydroponic system where recycled waste nutrient solution was photocatalytically treated with solar light and a system with untreated recycled waste nutrient solution. The results showed, as measured by growth indices such as stem length and stem thickness, that asparagus growth in the photocatalytically treated system was superior to the untreated one. Furthermore, the yield of asparagus spears was 1.6-fold greater in the photocatalytically treated system, demonstrating the detoxification effect on the phytotoxic compounds and also the killing effect on pathogenic microorganisms. PMID:18500814

  6. Hypocholesterolemic and Hepatoprotective Effects of “Triguero” Asparagus from Andalusia in Rats Fed a High Cholesterol Diet

    Directory of Open Access Journals (Sweden)

    M.D. García

    2012-01-01

    Full Text Available The cultivated species of the wild autochthonous Asparagus officinalis in Andalusia in Spain is commonly called “triguero” asparagus. This vegetable has traditionally been very much appreciated for its organoleptic and nutritional characteristics. This study has been designed to evaluate the potential effect of different concentrations of freeze-dried asparagus (500, 250, and 125 mg/Kg of body weight/day on oxidative status and lipid profile in rats fed a cholesterol-rich diet. After five weeks of treatment, doses of 250 and 500 mg/Kg of asparagus were able to significantly reduce total cholesterol and LDL cholesterol levels. Atherogenic index was also significantly reduced in a dose-dependent manner by administrating freeze-dried asparagus. A beneficial effect was observed in the HDL cholesterol levels in asparagus-fed groups although the increase was not significant. Consumption of asparagus also improved antioxidant status, assayed superoxide dismutase (SOD and catalase (CAT enzymes, and protected against lipid peroxidation. These results show that the intake of green asparagus from Andalusia (Spain helps to regulate plasma lipid levels and prevents oxidative damage in hypercholesterolemic conditions.

  7. Alergia al espárrago Allergy to the asparagus

    Directory of Open Access Journals (Sweden)

    A.I. Tabar

    2003-01-01

    Full Text Available El espárrago (Asparagus officinalis, ampliamente cultivado a escala mundial, forma parte, junto con el ajo, la cebolla y el puerro de la familia de Liliáceas. Se ha descrito hipersensibilidad al espárrago en forma de reacciones retardadas mediadas por células, y de reacciones inmediatas mediadas por IgE. Mientras que las primeras (dermatitis alérgica de contacto son una causa relativamente frecuente de patología ocupacional, la literatura sólo muestra casos puntuales de alergia IgE mediada. Las reacciones mediadas por IgE ocurren principalmente en individuos atópicos y se pueden clasificar en alergia alimentaria y reacciones secundarias a la exposición cutánea o inhalada, generalmente ocupacional, a proteínas del espárrago. La manifestación clínica más frecuente de la alergia alimentaria es la anafilaxia mientras que en las segundas, la urticaria de contacto, la rinitis y el asma ocupacional pueden coexistir o aparecer aisladas. La diferente forma de presentación clínica de las reacciones mediadas por IgE es probablemente consecuencia de la sensibilización a diferentes alergenos. La importancia de la detección y el diagnóstico diferencial de estas manifestaciones radica en su diferente tratamiento y pronóstico. En este artículo revisamos nuestra experiencia en los últimos 5 años.Asparagus (Asparagus officinalis is a widely grown vegetable; together with garlic, the onion and the leek, it belongs to the Liliaceae family. Both delayed cell-mediated reactions and IgE-mediated reactions secondary to asparagus have been described. While the former (allergic contact dermatitis are a fairly common cause of occupational disease, only a few case-reports of IgE-mediated reactions have been published. IgE-mediated reactions can be further grouped into food allergy and reactions due to cutaneous or respiratory exposure, which is often occupational. Anaphylaxis is the most common clinical picture of food allergy, while contact

  8. Morphological diversities of Asparagus officinalis L. germplasm resources%芦笋种质资源形态多样性分析

    Institute of Scientific and Technical Information of China (English)

    陈河龙; 张世清; 高建明; 郑金龙; 刘巧莲; 易克贤

    2016-01-01

    Through the morphological diversity analysis of 46 asparagus accessions, the result shows that first to three principal components represented 19.87%, 15.38%, and 11.92%. The length of secondary branches, diameter of main stem, number of secondary branches, diameter of secondary branches, diameter of primary branches, width of scale, length of spear scale, diameter of spear, fresh weight of spear, scale number below first panicle branch and plant height to first panicle branch are the main indicators of asparagus morphological differentiation. These factors are the focus of morphological traits selection on asparagus breeding. Based on the morphological cluster analysis, the 46 asparagus accessions were divided into 2 groups, the first group included Avalim, Pacific Endeavour, Pacific Peak, Backlim, Pacific Challenger 2, Shuofeng, JK107, Herkolim, Thielim, Jing lv No.1, JK101, Precoce and Pacific 2000. The second group included the other 33 asparagus accessions.%对46份芦笋种质进行形态多样性分析,结果表明:第一至第三主成分的方差贡献率分别为19.87%、15.38%、11.92%;二级分枝长度、主茎粗、二级分枝数、二级分枝粗、一级分枝粗、鳞片宽、笋鳞片长度、笋粗、笋鲜重、第1分枝以下的鳞片数量、第1分枝高度是芦笋形态分化的主要指标,也是芦笋选育种形态性状选择上关注的重点。基于形态性状的聚类分析,将46份芦笋种质分为两大类: Avalim、 Pacific Endeavour、Pacific Peak、 Backlim、 Pacific Challenger 2、硕丰、 JK107、 Herkolim、 Thielim、京绿芦1号、 JK101、Precoce、 Pacific 2000归为一类,其余33份种质归为一类。

  9. Alergia al espárrago Allergy to the asparagus

    OpenAIRE

    A.I. Tabar; M.J. Álvarez; E. Celay; López, R.; B. de Esteban; B. Gómez

    2003-01-01

    El espárrago (Asparagus officinalis), ampliamente cultivado a escala mundial, forma parte, junto con el ajo, la cebolla y el puerro de la familia de Liliáceas. Se ha descrito hipersensibilidad al espárrago en forma de reacciones retardadas mediadas por células, y de reacciones inmediatas mediadas por IgE. Mientras que las primeras (dermatitis alérgica de contacto) son una causa relativamente frecuente de patología ocupacional, la literatura sólo muestra casos puntuales de alergia IgE mediada....

  10. Ground penetrating radar for asparagus detection

    Science.gov (United States)

    Seyfried, Daniel; Schoebel, Joerg

    2016-03-01

    Ground penetrating radar is a promising technique for detection of buried objects. Recently, radar has more and more been identified to provide benefits for a plurality of applications, where it can increase efficiency of operation. One of these fields is the industrial automatic harvesting process of asparagus, which is performed so far by cutting the soil ridge at a certain height including all the asparagus spears and subsequently sieving the latter out of the soil. However, the height where the soil is cut is a critical parameter, since a wrong value leads to either damage of the roots of the asparagus plants or to a reduced crop yield as a consequence of too much biomass remaining in the soil. In this paper we present a new approach which utilizes ground penetrating radar for non-invasive sensing in order to obtain information on the optimal height for cutting the soil. Hence, asparagus spears of maximal length can be obtained, while keeping the roots at the same time undamaged. We describe our radar system as well as the subsequent digital signal processing steps utilized for extracting the information required from the recorded radar data, which then can be fed into some harvesting unit for setting up the optimal cutting height.

  11. Impacto de técnicas innovadoras aplicadas a la cadena agroalimentaria espárrago (Asparagus officinalis L. para optimizar el posicionamiento en diferentes mercados

    Directory of Open Access Journals (Sweden)

    Andrea P. Guisolis

    2010-12-01

    Full Text Available El espárrago (Asparagus officinalis L. es un producto delicado cuyo destino son segmentos de mercados muy exigentes en la presentación y cada vez más proclives a la adquisición de productos IV Gama, presentados en bandeja y listos para consumir. Actualmente, existen técnicas innovadoras de manejo productivo (empleo de plantines y manejo poscosecha (empleo de bandejas. Con el objetivo de estudiar el impacto sobre el rendimiento de densidades (D1: 35.714; D2: 23.810 y D3: 17.857 pl/ha, tamaños de plantines PG:celda grande (70 cm3, PM:celda mediana (50 cm3 y PP:celda pequeña (20 cm3 y la pérdida de peso fresco durante la poscosecha de turiones de distintos calibres (Large L, Medium M y Small S acondicionados en bandejas con (CF y sin film (SF, se realizó un ensayo, en la Facultad de Agronomía, UNCPBA, en donde se evaluaron las variables: productividad neta comercial (PNCH (kg/ha; por cosecha (PNCC (kg/cosecha; Nº de turiones (NT y peso promedio por turión (PPT. PNCH fue 1º año: 8012,6 kg/ha; 2º año: 4468,86 kg/ha (p < 0,05, mientras que PG:5.757, PM:6.213 y PP:6.688 kg/ha. El PPT de PG:10,46; PM:9,86 y PP:10,77 g/turión. D1 produjo 1069,14 kg/ha más y la mayor cantidad de turiones/ha (648,033. El peso fresco de los turiones acondicionados CF resultó estadísticamente superior (p < 0,05, obteniéndose en promedio 13,0 y para SF 10,45 g/turión. Respecto de los calibres los mayores perdieron menos peso (CF-L:5; CF-M:5; CF-S:9,1 %; SF-L:26; SF-M:35; SF-S: 36,36 %. Solo se conservaron características organolépticas en CF; L:50, M:28,57 y S:14,28 %. Por lo expuesto convendría utilizar D1 y el empleo de bandejas para su acondicionamiento.

  12. Study on Control Technology of Crioceris quatuordecimpunctata in Asparagus officinalis Field%芦笋十四点负泥虫防治技术研究

    Institute of Scientific and Technical Information of China (English)

    赵聚莹; 贾海民; 蒋晓丽; 陈丹

    2013-01-01

    The control efficacy of different dosage of six types of pesticides ( 25% beta-cypermethrin · phoxim EC, 4.5% cypermethrin EC, 40% phoxim EC, 2.5% deltamethrin EC, 2.5% cyhalothrin EC and 1.8%avermectin EC) on Crioceris quatuordecimpunctata in Asparagus officinalis L.field were studied.The results showed that the control efficacy of 25% beta-cypermethrin · phoxim EC 300 mL/hm2 , 4.5% cypermethrin EC 225 mL/hm2 , 2.5%deltamethrin EC 225 mL/hm2 and 2.5% cyhalothrin EC 150 mL/hm2 were more than 93.2%, and these pesticides had the characteristics of rapidly available, longer persistent period and lower cost, which were recommended application in the production.%  针对芦笋田害虫十四点负泥虫,进行了6种杀虫剂(25%高氯·辛乳油、4.5%高效氯氰菊酯乳油、40%辛硫磷乳油、2.5%溴氰菊酯乳油、2.5%功夫菊酯乳油和1.8%阿维菌素乳油)不同施药量的田间防效试验。结果表明:施药后7 d,25%高氯·辛乳油300 mL/hm2、4.5%高效氯氰菊酯乳油225 mL/hm2、2.5%溴氰菊酯乳油225 mL/hm2和2.5%功夫菊酯乳油150 mL/hm2防治效果较理想,防效均>93.2%,并且速效性好、持效期长、成本低,建议在生产上推广应用。

  13. Impacto de técnicas innovadoras aplicadas a la cadena agroalimentaria espárrago (Asparagus officinalis L. para optimizar el posicionamiento en diferentes mercados

    Directory of Open Access Journals (Sweden)

    Alicia Zubiría

    2010-01-01

    Full Text Available El espárrago (Asparagus officinalis L. es un producto delicado cuyo destino son segmentos de mercados muy exigentes en la presentación y cada vez más proclives a la adquisición de productos IV Gama, presentados en bandeja y listos para consumir. Actualmente, existen técnicas innovadoras de manejo productivo (empleo de plantines y manejo poscosecha (empleo de bandejas. Con el objetivo de estudiar el impacto sobre el rendimiento de densidades (D1: 35.714; D2: 23.810 y D3: 17.857 pl/ha, tamaños de plantines PG:celda grande (70 cm E3, PM:celda mediana (50 cm E3 y PP:celda pequeña (20 cm E3 y la pérdida de peso fresco durante la poscosecha de turiones de distintos calibres (Large L, Medium M y Small S acondicionados en bandejas con (CF y sin film (SF, se realizó un ensayo, en la Facultad de Agronomía, UNCPBA, en donde se evaluaron las variables: productividad neta comercial (PNCH (kg/ha; por cosecha (PNCC (kg/cosecha; Nº de turiones (NT y peso promedio por turión (PPT. PNCH fue 1º año: 8012,6 kg/ha; 2º año: 4468,86 kg/ha (p < 0,05, mientras que PG:5.757, PM:6.213 y PP:6.688 kg/ha. El PPT de PG:10,46; PM:9,86 y PP:10,77 g/turión. D1 produjo 1069,14 kg/ha más y la mayor cantidad de turiones/ha (648,033. El peso fresco de los turiones acondicionados CF resultó estadísticamente superior (p < 0,05, obteniéndose en promedio 13,0 y para SF 10,45 g/turión. Respecto de los calibres los mayores perdieron menos peso (CF-L:5; CF-M:5; CF-S:9,1 %; SF-L:26; SF-M:35; SF-S: 36,36 %. Solo se conservaron características organolépticas en CF; L:50, M:28,57 y S:14,28 %. Por lo expuesto convendría utilizar D1 y el empleo de bandejas para su acondicionamiento.

  14. 大孔吸附树脂纯化芦笋皂苷工艺优化%Purification of saponins from Asparagus officinalis by macroporous adsorption resin

    Institute of Scientific and Technical Information of China (English)

    赵芳春; 赵兵; 黄云祥

    2012-01-01

    An optimal macroporous adsorption resin and its optimal technological parameters for purification of saponins from extracts of Asparagus officinalis were investigated in the present study.Through static method,comparing the saponin purification result of 8 resins,HPD-100 resin was chosen due to its excellent adsorption and desorption performance.Optimal conditions were primarily confirmed as follows:sample concentration 15mg/mL with loading amount of 150mL at a flow rate of 1mL/min,washing with 200mL 80% ethanol at a flow rate of 1mL/min.Sample was purified by HPD-100 resin according to these technology parameters and the content of saponins reached 35.49%.Then the primary purification product was purified in accordance with the above process,and the content of saponins could be higher than 50% and complied with the standards of class V of new Chinese drug.These datas demonstrated the excellence of HPD-100 resin in purifying saponins from A.officinalis and the advantages of the developed method for industrial application,like simple operation,safety and low cost.%以芦笋下脚料提取物作为原料,优化了大孔吸附树脂纯化芦笋皂苷的工艺。通过静态法,对8种大孔吸附树脂纯化芦笋皂苷的效果进行比较,结果表明HPD-100树脂纯化芦笋皂苷效果最佳,并对HPD-100大孔树脂纯化芦笋皂苷工艺进行了优化。在上样质量浓度15mg/mL、溶液处理量150mL、1mL/min流速过柱;吸附后,先用50mL蒸馏水洗去杂质,再用200mL80%乙醇洗脱,总皂苷的含量由14.26%提高到35.49%(mean±SD)。将初次纯化产物按照上述工艺再纯化一次,总皂苷含量可高于50%,达到国家五类新药的含量要求。HPD-100可较好地吸附分离芦笋皂苷,纯化效率高,且其操作简单、安全、成本低廉,有较高的推广应用价值。

  15. Chemical, Physicochemical and Functional Characteristics of Dietary Fiber Obtained From Asparagus byproducts (Asparagus officinalis L. / Características Químicas, Físicoquímicas y Funcionales de la Fibra Dietaria Obtenida de los Subproductos del Espá

    Directory of Open Access Journals (Sweden)

    Edith Lorena Agudelo Cadavid

    2014-12-01

    Full Text Available Abstract. Due to its mechanical and biochemical properties,dietary fiber is part of a healthy diet. Containing good levels ofprebiotics, asparagus (Asparagus officinalis L. produces healthyeffects when incorporated into processed foods. The objective ofthe current study was to obtain fiber from asparagus byproductsand determine its chemical composition [Total Dietary Fiber (TDF,protein, moisture and ash] and physicochemical [pH, and wateractivity (aw] and functional characteristics [Water AbsorptionIndex (WAI, Water Solubility Index (WSI, Oil Absorption Index(OAI and Swelling Volume (SV]. The plant material was treatedthrough extraction and dehydration thermal treatments. Aresponse surface model was applied to evaluate the effects ofextraction and drying temperatures. The TDF ranged from 60.7 to79%. Significant differences were only observed for TDF, moistureand aw (P ≤ 0.05. The WAI, WSI, OAI and SV were found to bewithin the range observed for similar plant materials. Due totheir functional properties and elevated TDF content, asparagusbyproducts can be used as active biological components in foodproduction. This innovative utilization will contribute to reducingthe environmental impact of the industrial processing of thisvegetable. / Resumen. Por sus propiedades mecánicas y bioquímicas, la fibradietética puede formar parte de una alimentación saludable. Alser incorporado en los alimentos en el proceso de elaboración, elespárrago (Asparagus officinalis L. tiene efectos benéficos parala salud del consumidor, debido a su contenido de prebióticos. Elobjetivo del estudio fue obtener fibra a partir de los subproductos delespárrago para determinar su composición química [Fibra DietariaTotal (FDT, proteína, humedad y cenizas] y sus propiedadesfísicoquímicas [pH y actividad del agua (aw] y funcionales [Índicede Absorción del Agua (IAA, Índice de Solubilidad del Agua (ISA,Índice de Absorción de Aceite (IAAc y Volumen de

  16. Identification of Marchfeld asparagus using Sr isotope ratio measurements by MC-ICP-MS

    Energy Technology Data Exchange (ETDEWEB)

    Swoboda, S.; Brunner, M.; Boulyga, S.F.; Galler, P.; Prohaska, T. [University of Natural Resources and Applied Life Sciences, Department of Chemistry-VIRIS Project, Vienna (Austria); Horacek, M. [Austrian Research Centers GmbH, Seibersdorf (Austria)

    2008-01-15

    This work focuses on testing and application of Sr isotope signatures for the fast and reliable authentication and traceability of Asparagus officinalis originating from Marchfeld, Austria, using multicollector inductively coupled plasma mass spectrometry after optimised Rb/Sr separation. The major sample pool comprises freeze-dried and microwave-digested asparagus samples from Hungary and Slovakia which are compared with Austrian asparagus originating from the Marchfeld region, which is a protected geographical indication. Additional samples from Peru, the Netherlands and Germany were limited in number and allowed therefore only restricted statistical evaluation. Asparagus samples from Marchfeld were harvested within two subsequent years in order to investigate the annual variation. The results show that the Sr isotope ratio is consistent within these 2 years of investigation. Moreover, the Sr isotope ratio of total Sr in soil was found to be significantly higher than in an NH{sub 4}NO{sub 3} extract, reflecting the mobile (bioavailable) phase. The isotope composition in the latter extract corresponds well to the range found in the asparagus samples in Marchfeld, even though the concentration of Sr in asparagus shows no direct correlation to the concentration of Sr in the mobile phase of the soil. The major question was whether the 'Marchfelder Spargel' can be distinguished from samples from the neighbouring countries of Hungary and Slovakia. According to our findings, they can be clearly (100%) singled out from the Hungarian samples and can be distinguished from the Slovakian asparagus samples with a probability of more than 80%. (orig.)

  17. Asparagus Spears as a Model to Study Heteroxylan Biosynthesis during Secondary Wall Development.

    Science.gov (United States)

    Song, Lili; Zeng, Wei; Wu, Aimin; Picard, Kelsey; Lampugnani, Edwin R; Cheetamun, Roshan; Beahan, Cherie; Cassin, Andrew; Lonsdale, Andrew; Doblin, Monika S; Bacic, Antony

    2015-01-01

    Garden asparagus (Asparagus officinalis L.) is a commercially important crop species utilized for its excellent source of vitamins, minerals and dietary fiber. However, after harvest the tissue hardens and its quality rapidly deteriorates because spear cell walls become rigidified due to lignification and substantial increases in heteroxylan content. This latter observation prompted us to investigate the in vitro xylan xylosyltransferase (XylT) activity in asparagus. The current model system for studying heteroxylan biosynthesis, Arabidopsis, whilst a powerful genetic system, displays relatively low xylan XylT activity in in vitro microsomal preparations compared with garden asparagus therefore hampering our ability to study the molecular mechanism(s) of heteroxylan assembly. Here, we analyzed physiological and biochemical changes of garden asparagus spears stored at 4 °C after harvest and detected a high level of xylan XylT activity that accounts for this increased heteroxylan. The xylan XylT catalytic activity is at least thirteen-fold higher than that reported for previously published species, including Arabidopsis and grasses. A biochemical assay was optimized and up to seven successive Xyl residues were incorporated to extend the xylotetraose (Xyl4) acceptor backbone. To further elucidate the xylan biosynthesis mechanism, we used RNA-seq to generate an Asparagus reference transcriptome and identified five putative xylan biosynthetic genes (AoIRX9, AoIRX9-L, AoIRX10, AoIRX14_A, AoIRX14_B) with AoIRX9 having an expression profile that is distinct from the other genes. We propose that Asparagus provides an ideal biochemical system to investigate the biochemical aspects of heteroxylan biosynthesis and also offers the additional benefit of being able to study the lignification process during plant stem maturation.

  18. Asparagus Spears as a Model to Study Heteroxylan Biosynthesis during Secondary Wall Development.

    Directory of Open Access Journals (Sweden)

    Lili Song

    Full Text Available Garden asparagus (Asparagus officinalis L. is a commercially important crop species utilized for its excellent source of vitamins, minerals and dietary fiber. However, after harvest the tissue hardens and its quality rapidly deteriorates because spear cell walls become rigidified due to lignification and substantial increases in heteroxylan content. This latter observation prompted us to investigate the in vitro xylan xylosyltransferase (XylT activity in asparagus. The current model system for studying heteroxylan biosynthesis, Arabidopsis, whilst a powerful genetic system, displays relatively low xylan XylT activity in in vitro microsomal preparations compared with garden asparagus therefore hampering our ability to study the molecular mechanism(s of heteroxylan assembly. Here, we analyzed physiological and biochemical changes of garden asparagus spears stored at 4 °C after harvest and detected a high level of xylan XylT activity that accounts for this increased heteroxylan. The xylan XylT catalytic activity is at least thirteen-fold higher than that reported for previously published species, including Arabidopsis and grasses. A biochemical assay was optimized and up to seven successive Xyl residues were incorporated to extend the xylotetraose (Xyl4 acceptor backbone. To further elucidate the xylan biosynthesis mechanism, we used RNA-seq to generate an Asparagus reference transcriptome and identified five putative xylan biosynthetic genes (AoIRX9, AoIRX9-L, AoIRX10, AoIRX14_A, AoIRX14_B with AoIRX9 having an expression profile that is distinct from the other genes. We propose that Asparagus provides an ideal biochemical system to investigate the biochemical aspects of heteroxylan biosynthesis and also offers the additional benefit of being able to study the lignification process during plant stem maturation.

  19. Asparagus Spears as a Model to Study Heteroxylan Biosynthesis during Secondary Wall Development.

    Science.gov (United States)

    Song, Lili; Zeng, Wei; Wu, Aimin; Picard, Kelsey; Lampugnani, Edwin R; Cheetamun, Roshan; Beahan, Cherie; Cassin, Andrew; Lonsdale, Andrew; Doblin, Monika S; Bacic, Antony

    2015-01-01

    Garden asparagus (Asparagus officinalis L.) is a commercially important crop species utilized for its excellent source of vitamins, minerals and dietary fiber. However, after harvest the tissue hardens and its quality rapidly deteriorates because spear cell walls become rigidified due to lignification and substantial increases in heteroxylan content. This latter observation prompted us to investigate the in vitro xylan xylosyltransferase (XylT) activity in asparagus. The current model system for studying heteroxylan biosynthesis, Arabidopsis, whilst a powerful genetic system, displays relatively low xylan XylT activity in in vitro microsomal preparations compared with garden asparagus therefore hampering our ability to study the molecular mechanism(s) of heteroxylan assembly. Here, we analyzed physiological and biochemical changes of garden asparagus spears stored at 4 °C after harvest and detected a high level of xylan XylT activity that accounts for this increased heteroxylan. The xylan XylT catalytic activity is at least thirteen-fold higher than that reported for previously published species, including Arabidopsis and grasses. A biochemical assay was optimized and up to seven successive Xyl residues were incorporated to extend the xylotetraose (Xyl4) acceptor backbone. To further elucidate the xylan biosynthesis mechanism, we used RNA-seq to generate an Asparagus reference transcriptome and identified five putative xylan biosynthetic genes (AoIRX9, AoIRX9-L, AoIRX10, AoIRX14_A, AoIRX14_B) with AoIRX9 having an expression profile that is distinct from the other genes. We propose that Asparagus provides an ideal biochemical system to investigate the biochemical aspects of heteroxylan biosynthesis and also offers the additional benefit of being able to study the lignification process during plant stem maturation. PMID:25894575

  20. Financial Assessment of a new Soil Heating Technology for Off-season Asparagus Production

    Directory of Open Access Journals (Sweden)

    A. Luce

    2006-01-01

    Full Text Available The financial effectiveness of a soil heating system placed under the crowns in each raised bed (UCR of Asparagus plants (Asparagus officinalis L. was evaluated and then compared with a traditional greenhouse production system (TPS, a plastic greenhouse without any heating system, in northern Greece. Several appraisal approaches, such as net present value, net present value adjusted for inflation and benefit/cost ratio, were used to evaluate the effectiveness of UCR system for off-season Asparagus production. Results demonstrate the importance of UCR system in terms of volume of production, gross returns and overall effectiveness. Finally, the performed sensitivity analysis indicates that product price variations alter the accrued benefits.

  1. Effect of Asparagus polysaccharide on the number and activity of erythrocyte complement receptor 1 (CD35) of S180 mice

    Institute of Scientific and Technical Information of China (English)

    JI Yu-bin; MIN Yong-cui; JI Chen-feng

    2008-01-01

    Objective To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythroeyte complement receptor 1 in S180 mice. Methods Red blood cells from mice venous blood were labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of ECR1. Using microscope, we studied the adherence between erythrocyte immunity and C3b receptor or tumor-cell by RBC-C3bRR and DTER. Results Comparing the mean value of the number of CR1 on each RBC of high and middle groups with control groups, the mean value of the number of CR1, RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups are increased significantly. Conclusions Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice, which may be one of its most important antitumor mechanisms.

  2. White Asparagus Harvest Date Discrimination Using Nirs Technology

    Science.gov (United States)

    Jarén, C.; Arazuri, S.; García, M. J.; Arnal, P.; Arana, J. I.

    2006-03-01

    Asparagus is still an important resource for mid-size and small farms. It has been traditionally believed that factors such as the asparagus harvesting date have an influence on its quality. This research sought to identify the harvesting dates of different fruits by using Near Infrared Spectroscopy (NIRS) technology as quality indicators and the best zone a long of the asparagus to acquire the spectrum. All the asparagus tested came from the same manufacturer but had been canned on three different dates, giving a total of nine lots. There were one hundred asparagus per lot and the experiment data were taken from three different parts (tip, middle, and base) of each spear. Reflectance spectrum in the near infrared between 800 1700 nm was determined. Differences NIRS among the asparagus harvested on different dates were found. NIRS technology was able to classify the asparagus correctly in terms of harvest dates (71% well classified). The base of the asparagus turned out to be the best part to use in order to establish the harvest date.

  3. Study on Biological Characters of Asparagus macowanii Baker.

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Asparagus macowanfi Baker, is a climbing herbaceous foliage species in genus Asparagus of Liliaceae, This paper summarized its multiple uses, morphologi- cal characteristics, biological habit, reproduction methods, management after cultiva- tion, prevention and control of pests and disease, as well as harvest and grading, with the objective to provide references for the exploitation and utilization of As- paragus macowanii Baker.

  4. Detection of kestoses and kestose-related oligosaccharides in extracts of Festuca arundinacea, Dactylis glomerate L. , and Asparagus officinalis L. root cultures and invertase by sup 13 C and sup 1 H nuclear magnetic resonance spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Forsythe, K.L.; Feather, M.S.; Gracz, H.; Wong, T.C. (Univ. of Missouri, Columbia (USA))

    1990-04-01

    Previous studies show that {sup 13}C nuclear magnetic resonance spectroscopy can be used to detect and identify mixtures of 1-kestose and neokestose after conversion to the acetate derivatives. In this study, unequivocal assignments are made for the anomeric carbon and proton signals for the above two trisaccharide acetates as well as for 6-kestose hendecaacetate and for nystose tetradecaacetate (a 1-kestose-derived tetrasaccharide). A number of oligosaccharide fractions were isolated from several plant species, converted to the acetates, and nuclear magnetic resonance spectra obtained. Using the above reference data, the following information was obtained. The trisaccharide fraction from Dactylis gomerata L. stem tissue and Asparagus officinalis L. roots contain both 1-kestose and neokestose, and the tetrasaccharide fractions contain three components, one of which is nystose. Penta- and hexasaccharide acetates were also isolated from A. officinalis L. roots and were found to contain, respectively, four and at least five components. All components of both of the above species appear to contain a kestose residue and to be produced by the sequential addition of fructofuranosyl units to these. The trisaccharide fraction from Festuca arundinacea is complex, and contains at least five different components, two of which appear to be 1-kestose and neokestose.

  5. Two New Furostanol Glycosides from Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Two new furostanol oligoglycosides named as aspacochioside A (1) and B (2) were isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Their structures were elucidated to be 3-O-[{α-L-rhamnopyranosyl-(1→4)}{β-D-glucopyranosyl}]-26-O-[β-D-glucopy- ranosyl]-(25S)-5β-furostane-3β,22α,26-triol 1 and 3-O-[{α-L-rhamnopyranosyl-(1→4)}{(-D-glu- copyranosyl}]-26-O-[β-D-glucopyranosyl]-22α-methoxy-(25S)-5β-furostane-3β,26-diol 2 on the basis of spectroscopic techniques and chemical methods.

  6. 基于火焰原子吸收光谱法的芦笋中微量元素含量的测定%Determination of Trace Elements in Asparagus(Asparagus officinalis L.) by Flame Atomic Absorption Spectrometry(FAAS)

    Institute of Scientific and Technical Information of China (English)

    弓巧娟; 杨海英; 郭桂珍

    2010-01-01

    [目的]用火焰原子吸收光谱法测定芦笋 (Asparagus officinalis L.)中Cu、Fe、Zn、Ca、Mg、K微量元素含量,为芦笋的合理开发利用提供依据.[方法]加入LaCl_3和CsCl消除测定Ca、Mg和K时的干扰,以硝酸和过氧化氢为消解剂,采用高压密闭消解火焰原子吸收光谱法测定白芦笋、白芦笋罐头、绿芦笋及其嫩茎和花中Cu、Fe、Zn、Ca、Mg、K元素含量.[结果]方法的回收率在91.2%~112.3%,相对标准偏差为1.43%~2.31%.芦笋加工为罐头后,Cu、Fe、Zn和Ca元素含量增大,K和Mg元素含量减少;绿芦笋中Cu、Fe、Zn、Ca、Mg、K元素含量均高于白芦笋;绿芦笋花中Cu、Zn、Ca和Mg含量明显大于茎中.[结论]该研究可为芦笋的合理开发与利用提供依据.

  7. Effect of chitosan coatings on postharvest green asparagus quality.

    Science.gov (United States)

    Qiu, Miao; Jiang, Hengjun; Ren, Gerui; Huang, Jianying; Wang, Xiangyang

    2013-02-15

    Fresh postharvest green asparagus rapidly deteriorate due to its high respiration rate. The main benefits of edible active coatings are their edible characteristics, biodegradability and increase in food safety. In this study, the quality of the edible coatings based on 0.50%, 0.25% high-molecular weight chitosan (H-chitosan), and 0.50%, 0.25% low-molecular weight chitosan (L-chitosan) on postharvest green asparagus was investigated. On the basis of the results obtained, 0.25% H-chitosan and 0.50% L-chitosan treatments ensured lower color variation, less weight loss and less ascorbic acid, decrease presenting better quality of asparagus than other concentrations of chitosan treatments and the control during the cold storage, and prolonging a shelf life of postharvest green asparagus.

  8. An Application of Image Analysis and Colorimetric Methods on Color Change of Dehydrated Asparagus (Asparagus maritimus L.

    Directory of Open Access Journals (Sweden)

    Jasmina Lukinac

    2009-12-01

    Full Text Available Shape and color are key factors in quality evaluation of fresh asparagus (Asparagus maritimus L.. Typical green color of asparagus comes from the chlorophyll, pigment which has been degradated during drying process. The aim of this paper was to compare color changes of asparagus dried in laboratory tray drier equipment at different temperatures (40 °C, 50 °C, 60 °C and 70 °C at airflow velocity of 2.75 ms-1. Color changes were obtained by digital image analysis in RGB color model and by chromameter in L*a*b* color model. Basic elements of image analysis system were low voltage halogen lamps with reflector, digital camera and programs for image pre-processing and analysis.Mean values of color parameters, color changes and correlation coefficients for asparagus were calculated for both color models. An analysis showed statistically significant influence of drying temperature on hue angle and total color change for both chosen color models of dehydrated asparagus. Represented results show that there was no statistically significant difference according to color changes between drying at 50 °C and 60 °C. Calculated correlation coefficient between color changes for used models was found to be 0.9167.

  9. 芦笋雌性特异性片段的克隆和分析(简报)%CLONING AND ANALYZING OF THE FEMALE-SPECIFIC MARKER IN THE DIOECIOUS SPECIES ASPARAGUS OFFICINALIS L.

    Institute of Scientific and Technical Information of China (English)

    卢龙斗; 李瑞丽; 高武军; 邓传良; 王连军

    2006-01-01

    芦笋(Asparagus officinalis L.)又名石刁柏、龙须菜。系雌雄异株宿根性植物,是重要的经济作物之一。芦笋的性染色体为一对同形的L5染色体,雌性的性染色体为XX。雄性的性染色体为XY。性别决定的多态性是由位于一对L5性染色体上的一个显性基因M决定的。雌株基因型为隐性纯合子mm.雄株为显性纯合子MM(又称超雄株)或杂合子Mm。在生产上。由于雄株比雌株高产25%以上。并具有极强的抗病性和生命力。故雄株特别是超雄株则倍受生产者的青睐.但芦笋雌雄鉴定只有等到种植的第二年待植株开花时才能进行。这就严重影响了芦笋的有目的种植和经济效益。

  10. Expression of Cry1Ac in transgenic tobacco plants under the control of a wound-inducible promoter (AoPR1) isolated from Asparagus officinalis to control Heliothis virescens and Manduca sexta.

    Science.gov (United States)

    Gulbitti-Onarici, Selma; Zaidi, Mohsin Abbas; Taga, Ibrahim; Ozcan, Sebahattin; Altosaar, Illimar

    2009-07-01

    Expression of cry1Ac gene from Bacillus thuringiensis (Bt) was evaluated under the control of a wound-inducible AoPR1 promoter from Asparagus officinalis in transgenic tobacco plants. The leaves of transgenic plants were mechanically wounded to evaluate the activity of the AoPR1 promoter in driving the expression of Cry1Ac protein at the wound site. Our results indicate that mechanical wounding of transgenic plants was effective in inducing the expression of Cry1Ac protein. As a result of this induction, the accumulated levels of Cry1Ac protein increased during 6-72 h post-wounding period. The leaves of transgenic tobacco plants were evaluated for resistance against Heliothis virescens and Manduca sexta in insect bioassays in two different ways. The detached tobacco leaves were either fed directly to the insect larvae or they were first mechanically wounded followed by a 72 h post-wounding feeding period. Complete protection of mechanically wounded leaves of transgenic plants was observed within 24 h of the bioassay. The leaves of transgenic plants fed directly (without pre-wounding) to the larvae achieved the same level of protection between 24 and 72 h of the bioassay. PMID:19353306

  11. 芦笋抗肿瘤活性成分及作用机制的研究进展%Study progress on anti-tumor mechanism of active components in Asparagus officinalis

    Institute of Scientific and Technical Information of China (English)

    季宇彬; 许贺

    2008-01-01

    芦笋(Asparagus officinalis linn)为百合科天门冬属的多年生草本植物,学名石刁柏,又名龙须菜、文山竹、索罗罗、细百叶部。芦笋具有很高的营养保健及药用价值,神农本草经将野生芦笋——天门冬列为“上品之上”。研究发现,芦笋活性成分具有抗肿瘤、免疫调节、抗衰老抗疲劳、降血脂、保肝解毒等特殊的生物学功能。有关芦笋活性成分的研究已引起了国内外学者的高度关注。

  12. Asparagus racemosus--ethnopharmacological evaluation and conservation needs.

    Science.gov (United States)

    Bopana, Nishritha; Saxena, Sanjay

    2007-03-01

    Asparagus racemosus Willd. (Asparagaceae) is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani and Siddha. Asparagus racemosus is mainly known for its phytoestrogenic properties. With an increasing realization that hormone replacement therapy with synthetic oestrogens is neither as safe nor as effective as previously envisaged, the interest in plant-derived oestrogens has increased tremendously making Asparagus racemosus particularly important. The plant has been shown to aid in the treatment of neurodegenerative disorders and in alcohol abstinence-induced withdrawal symptoms. In Ayurveda, Asparagus racemosus has been described as a rasayana herb and has been used extensively as an adaptogen to increase the non-specific resistance of organisms against a variety of stresses. Besides use in the treatment of diarrhoea and dysentery, the plant also has potent antioxidant, immunostimulant, anti-dyspepsia and antitussive effects. Due to its multiple uses, the demand for Asparagus racemosus is constantly on the rise; however, the supply is rather erratic and inadequate. Destructive harvesting, combined with habitat destruction in the form of deforestation has aggravated the problem. The plant is now considered 'endangered' in its natural habitat. Therefore, the need for conservation of this plant is crucial. This article aims to evaluate the biological activities, pharmacological applications and clinical studies of Asparagus racemosus in an attempt to provide a direction for further research. Keeping in mind the fact that it is the active principle that imparts medicinal value to a plant; consistency in quality and quantity needs to be maintained to ensure uniform drug efficacy. Also, deliberate or inadvertent adulteration needs to be dealt with at an early stage. To overcome these prevalent problems, the availability

  13. Asparagus racemosus--ethnopharmacological evaluation and conservation needs.

    Science.gov (United States)

    Bopana, Nishritha; Saxena, Sanjay

    2007-03-01

    Asparagus racemosus Willd. (Asparagaceae) is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani and Siddha. Asparagus racemosus is mainly known for its phytoestrogenic properties. With an increasing realization that hormone replacement therapy with synthetic oestrogens is neither as safe nor as effective as previously envisaged, the interest in plant-derived oestrogens has increased tremendously making Asparagus racemosus particularly important. The plant has been shown to aid in the treatment of neurodegenerative disorders and in alcohol abstinence-induced withdrawal symptoms. In Ayurveda, Asparagus racemosus has been described as a rasayana herb and has been used extensively as an adaptogen to increase the non-specific resistance of organisms against a variety of stresses. Besides use in the treatment of diarrhoea and dysentery, the plant also has potent antioxidant, immunostimulant, anti-dyspepsia and antitussive effects. Due to its multiple uses, the demand for Asparagus racemosus is constantly on the rise; however, the supply is rather erratic and inadequate. Destructive harvesting, combined with habitat destruction in the form of deforestation has aggravated the problem. The plant is now considered 'endangered' in its natural habitat. Therefore, the need for conservation of this plant is crucial. This article aims to evaluate the biological activities, pharmacological applications and clinical studies of Asparagus racemosus in an attempt to provide a direction for further research. Keeping in mind the fact that it is the active principle that imparts medicinal value to a plant; consistency in quality and quantity needs to be maintained to ensure uniform drug efficacy. Also, deliberate or inadvertent adulteration needs to be dealt with at an early stage. To overcome these prevalent problems, the availability

  14. Combined RAPD and RFLP molecular linkage map of asparagus.

    Science.gov (United States)

    Jiang, C; Lewis, M E; Sink, K C

    1997-02-01

    Two linkage maps of asparagus (Asparagus officinalis L.) were constructed using a double pseudotestcross mapping strategy with restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), and allozymes as markers in a population generated from crossing MW25 x A19, two heterozygous parents. All data were inverted and combined with the natural data to detect linkages in repulsion phase. Two sets of data, one for each parent, were formed according to the inheritance patterns of the markers. The maternal MW25 map has a total of 163 marker loci placed in 13 linkage groups covering 1281 cM, with an average and a maximum distance between adjacent loci of 7.9 and 29 cM, respectively. The paternal A19 map has 183 marker loci covering 1324 cM in 9 linkage groups, with an average and a maximum distance between two adjacent loci of 7.7 and 29 cM, respectively. Six multiallelic RFLPs segregating in the pattern a/c x b/c and eight heterozygous loci (four RAPDs, and four RFLPs segregating in the pattern a/b x a/b (HZ loci)) were common to both maps. These 14 loci were used as bridges to align homologous groups between the two maps. In this case, RFLPs were more frequent and informative than RAPDs. Nine linkage groups in the MW25 map were homologous to six groups in the A19 map. In two cases, two or more bridge loci were common to a group; thus, the orientation of homologous linkage groups was also determined. In four other cases, only one locus was common to the two homologous groups and the orientation was unknown. Mdh, four RFLPs, and 14 RAPDs were assigned to chromosome L5, which also has the sex locus M. PMID:18464808

  15. Three new species of Asparagus (Asparagaceae from South Africa, with notes on other taxa

    Directory of Open Access Journals (Sweden)

    S. M. Burrows

    2008-12-01

    Full Text Available Three new species of Asparagus L. are described from South Africa: Asparagus elephantinus S.M.Burrows, A. hirsutus S.M.Burrows and A. sylvicola S.M.Burrows; and the past confusion between Asparagus acocksii Jessop and A. lynetteae (Oberm. Fellingham & N.L.Mey. is discussed.

  16. The Genus Asparagus in Southern Africa*

    Directory of Open Access Journals (Sweden)

    J. P. Jessop

    1966-12-01

    Full Text Available A revision of the genus Asparagus in South Africa, South West Africa, Bechuanaland, Basutoland, and Swaziland has been undertaken. Notes are given on the value of most o f the characters which have been used in the separation of species, recent literature on the nature of  Asparagus assimilatory organs, and a technique for the examination of chromosomes. Chromosome counts are listed for ten taxa. There is a key to the forty species and four varieties. In the main part of the work these taxa are described, and their synonymy, taxonomy, distribution and habitats dealt with. Six species and one variety are new. The following are the new species and combinations:  A. setaceus (Kunth (Asparagopsis setacea Kunth, A. mueronatus, A. macowanii Bak. var. zuluensis (N. E. Br.  (A. zuluensis N. E. Br., A. rigidus, A. densiflorus (Kunth  {Asparagopsis densiflora Kunth,  A. aethiopicus L. var. angusticladus, A. falcatus L. var. ternifolius (Bak.  (A. aethiopicus L. var.  ternifolius Bak.,  A. aspergillus,  A. obermeyerae,  A. krebsianus (Kunth (Asparagopsis krebsiana Kunth,  A. acocksii.  A. crassicladus. Several plants o f horticultural importance occur in South Africa. The three best known are A. plumosus, which is reduced here to synonymy under  A. setaceus (Kunth Jessop, and  A. sprengeri and  A. myersii. A. sprengeri is being reduced to synonymy under  A. densiflorus (Kunth Jessop,.  A. myersii, which is a  nomen nudum, is also regarded as belonging to  A. densiflorus.

  17. DAMAGE RESEARCH WITH P. PENETRANS IN ASPARAGUS PLANTS.

    Science.gov (United States)

    Hoek, J; Molendijk, L P G

    2014-01-01

    During cultivation of asparagus plants growth can be inhibited and yield can be reduced by plant-parasitic nematodes. Plant raising companies assume that the root lesion nematode (Pratylenchus penetrans) can cause severe yield loss in asparagus plants. However quantitative information about yield reduction in relation to the degree of infestation of this nematode species in the field is lacking. Research was done in The Netherlands by Applied Plant Research (part of Wageningen University and Research Centre) to determine the maximum degree of yield loss of asparagus plants at high infestation levels of P. penetrans and to establish the height of the tolerance limit for this nematode species. Also was investigated whether a field application of a granular nematicide could prevent or reduce yield loss caused by P. penetrans. Research was done in the field at sandy soils at the PPO location near Vredepeel in The Netherlands over a period of two years. In the first year the most suitable field was selected and on this field different infestation levels of P. penetrans were created. In the second year asparagus was cultivated and plant yield (number and quality of deliverable plants and financial yield) was calculated. At high infestation levels of Pratylenchus penetrans maximum yield loss was 12% (which can mean a financial loss of 7.000 C per ha). Yield started to decrease at very low infestation levels of P. penetrans and no statistical reliable tolerance limit could be calculated. Field application of 40 kg per ha of Vydate 10 G just before sowing of asparagus, could almost completely prevent yield loss caused by P. penetrans. After harvest infestation levels of P. penetrans were much lower than could be expected if asparagus was a non-host for this nematode species. In this paper therefore it is suggested that asparagus plants are (actively) controlling P. penetrans.

  18. Acquisition and diversification of cladodes: leaf-like organs in the genus Asparagus.

    Science.gov (United States)

    Nakayama, Hokuto; Yamaguchi, Takahiro; Tsukaya, Hirokazu

    2012-03-01

    The genus Asparagus is unusual in producing axillary, determinate organs called cladodes, which may take on either a flattened or cylindrical form. Here, we investigated the evolution of cladodes to elucidate the mechanisms at play in the diversification of shoot morphology. Our observations of Asparagus asparagoides, which has leaf-like cladodes, showed that its cladodes are anatomically and developmentally similar to leaves but differ in the adaxial/abaxial polarity of the vasculature. In addition to the expression of an ortholog of KNAT1, orthologous genes that are normally expressed in leaves, asymmetric leaves1 and HD-ZIPIII, were found to be expressed in cladode primordia in a leaf-like manner. The cylindrical cladodes of Asparagus officinalis showed largely similar expression patterns but showed evidence of being genetically abaxialized. These results provide evidence that cladodes are modified axillary shoots, suggest that the co-option of preexisting gene networks involved in leaf development transferred the leaf-like form to axillary shoots, and imply that altered expression of leaf polarity genes led to the evolution of cylindrical cladodes in the A. officinalis clade.

  19. Acquisition and diversification of cladodes: leaf-like organs in the genus Asparagus.

    Science.gov (United States)

    Nakayama, Hokuto; Yamaguchi, Takahiro; Tsukaya, Hirokazu

    2012-03-01

    The genus Asparagus is unusual in producing axillary, determinate organs called cladodes, which may take on either a flattened or cylindrical form. Here, we investigated the evolution of cladodes to elucidate the mechanisms at play in the diversification of shoot morphology. Our observations of Asparagus asparagoides, which has leaf-like cladodes, showed that its cladodes are anatomically and developmentally similar to leaves but differ in the adaxial/abaxial polarity of the vasculature. In addition to the expression of an ortholog of KNAT1, orthologous genes that are normally expressed in leaves, asymmetric leaves1 and HD-ZIPIII, were found to be expressed in cladode primordia in a leaf-like manner. The cylindrical cladodes of Asparagus officinalis showed largely similar expression patterns but showed evidence of being genetically abaxialized. These results provide evidence that cladodes are modified axillary shoots, suggest that the co-option of preexisting gene networks involved in leaf development transferred the leaf-like form to axillary shoots, and imply that altered expression of leaf polarity genes led to the evolution of cylindrical cladodes in the A. officinalis clade. PMID:22415273

  20. A preliminary study on the identification of male-asparagus and female-asparagus plants%芦笋雌雄株鉴定方法初探

    Institute of Scientific and Technical Information of China (English)

    冯莉; 寿森炎; 李敏

    2003-01-01

    @@ 芦笋(Asparagus officinalis L.),又名石刁柏、龙须菜,属百合科天门冬属多年生草本植物.芦笋嫩茎营养丰富,脆而多汁,风味独特,是一种具有较高药用、营养和保健价值蔬菜.近几年,芦笋已成为"世界蔬菜之王",我国种植面积也逐年扩大.

  1. Antiradical capacity and polyphenol composition of asparagus spears varieties cultivated under diff erent sunlight conditions

    Directory of Open Access Journals (Sweden)

    Bartosz Kulczyński

    2016-09-01

    Full Text Available Background. Asparagus offi cinalis has a high nutritional value. Asparagus is rich in a number of bioactive compounds, mainly fl avonoids (quercetin, glutathione, vitamin C, vitamin E, fructans (inulin and fructooligosaccharides and phytosterols (b-sitosterol. These compounds may play an important role in human health. The purpose of this study was to examine the antioxidant potential and polyphenol composition of white, pale-colored and green asparagus spears of diff erent cultivars. Material and methods. Investigations were conducted on diff erent asparagus spear extracts. The study included three colors of asparagus (white, pale-colored and green from fi ve diff erent cultivars subjected to the ethanol extraction procedure. Total phenolic content was also determined by the Folin-Ciocalteu method. Polyphenol (phenolic acids and fl avonols composition was estimated using the HPLC method. The antioxidant properties of extracts were examined using DPPH, ABTS and metal ion chelating assays. Results. The highest contents of phenolic and fl avonoids were observed in green asparagus from Grolim and the lowest in pale-colored asparagus from Gyjmlin. It was found that both the color of asparagus and the cultivar had a signifi cant eff ect on the composition of phenolic acid and fl avonols. Radical scavenging activity toward DPPH• and ABTS was highest for green asparagus cv. Grolim and Eposs. The greatest number of Fe ions was chelated by samples of green asparagus cv. Grolim and Huchel’s Alpha and pale-colored asparagus cv. Huchel’s Alpha. Conclusion. It was shown that the antioxidant activity of asparagus spears measured by antiradical and chelating activity test depends on variety and color. The highest activity was found in green asparagus and the lowest was identifi ed in white asparagus extracts. It has also been clarifi ed that changes in fl avonol and phenolic acid composition and increases in their diversity depends on growing with

  2. Bioactive constituents from "triguero" asparagus improve the plasma lipid profile and liver antioxidant status in hypercholesterolemic rats.

    Science.gov (United States)

    Vázquez-Castilla, Sara; De la Puerta, Rocío; Garcia Gimenez, María Dolores; Fernández-Arche, María Angeles; Guillén-Bejarano, Rafael

    2013-10-24

    We have previously shown that the Andalusian-cultivated Asparagus officinalis L. "triguero" variety produces hypocholesterolemic and hepatoprotective effects on rats. This asparagus is a rich source of phytochemicals although we hypothesized there would be some of them more involved in these functional properties. Thus, we aimed to study the effects of asparagus (500 mg/kg body weight (bw)/day) and their partially purified fractions in flavonoids (50 mg/kg bw/day), saponins (5 mg/kg bw/day) and dietary fiber (500 mg/kg bw/day) on oxidative status and on lipid profile in rats fed a cholesterol-rich diet. After 5 weeks treatment, plasma lipid values, hepatic enzyme activities and liver malondialdehyde (MDA) concentrations were measured. With the exception of the saponin fraction (SF), the administration of lyophilized asparagus (LA), fiber fraction (FF), and flavonoid fraction (FVF) to hypercholesterolemic rats produced a significant hypolipidemic effect compare to a high-cholesterol diet (HCD). In addition, the LA and FVF groups exhibited a significant increase in enzyme activity from multiple hepatic antioxidant systems including: superoxide dismutase, catalase, and gluthatione reductase/peroxidase as well as a decrease in MDA concentrations compared to HCD group. These results demonstrate that "triguero" asparagus possesses bioactive constituents, especially dietary fiber and flavonoids, that improve the plasma lipid profile and prevent hepatic oxidative damage under conditions of hypercholesterolemia.

  3. Bioactive Constituents from “Triguero” Asparagus Improve the Plasma Lipid Profile and Liver Antioxidant Status in Hypercholesterolemic Rats

    Directory of Open Access Journals (Sweden)

    Rafael Guillén-Bejarano

    2013-10-01

    Full Text Available We have previously shown that the Andalusian-cultivated Asparagus officinalis L. “triguero” variety produces hypocholesterolemic and hepatoprotective effects on rats. This asparagus is a rich source of phytochemicals although we hypothesized there would be some of them more involved in these functional properties. Thus, we aimed to study the effects of asparagus (500 mg/kg body weight (bw/day and their partially purified fractions in flavonoids (50 mg/kg bw/day, saponins (5 mg/kg bw/day and dietary fiber (500 mg/kg bw/day on oxidative status and on lipid profile in rats fed a cholesterol-rich diet. After 5 weeks treatment, plasma lipid values, hepatic enzyme activities and liver malondialdehyde (MDA concentrations were measured. With the exception of the saponin fraction (SF, the administration of lyophilized asparagus (LA, fiber fraction (FF, and flavonoid fraction (FVF to hypercholesterolemic rats produced a significant hypolipidemic effect compare to a high-cholesterol diet (HCD. In addition, the LA and FVF groups exhibited a significant increase in enzyme activity from multiple hepatic antioxidant systems including: superoxide dismutase, catalase, and gluthatione reductase/peroxidase as well as a decrease in MDA concentrations compared to HCD group. These results demonstrate that “triguero” asparagus possesses bioactive constituents, especially dietary fiber and flavonoids, that improve the plasma lipid profile and prevent hepatic oxidative damage under conditions of hypercholesterolemia.

  4. Bioactive constituents from "triguero" asparagus improve the plasma lipid profile and liver antioxidant status in hypercholesterolemic rats.

    Science.gov (United States)

    Vázquez-Castilla, Sara; De la Puerta, Rocío; Garcia Gimenez, María Dolores; Fernández-Arche, María Angeles; Guillén-Bejarano, Rafael

    2013-01-01

    We have previously shown that the Andalusian-cultivated Asparagus officinalis L. "triguero" variety produces hypocholesterolemic and hepatoprotective effects on rats. This asparagus is a rich source of phytochemicals although we hypothesized there would be some of them more involved in these functional properties. Thus, we aimed to study the effects of asparagus (500 mg/kg body weight (bw)/day) and their partially purified fractions in flavonoids (50 mg/kg bw/day), saponins (5 mg/kg bw/day) and dietary fiber (500 mg/kg bw/day) on oxidative status and on lipid profile in rats fed a cholesterol-rich diet. After 5 weeks treatment, plasma lipid values, hepatic enzyme activities and liver malondialdehyde (MDA) concentrations were measured. With the exception of the saponin fraction (SF), the administration of lyophilized asparagus (LA), fiber fraction (FF), and flavonoid fraction (FVF) to hypercholesterolemic rats produced a significant hypolipidemic effect compare to a high-cholesterol diet (HCD). In addition, the LA and FVF groups exhibited a significant increase in enzyme activity from multiple hepatic antioxidant systems including: superoxide dismutase, catalase, and gluthatione reductase/peroxidase as well as a decrease in MDA concentrations compared to HCD group. These results demonstrate that "triguero" asparagus possesses bioactive constituents, especially dietary fiber and flavonoids, that improve the plasma lipid profile and prevent hepatic oxidative damage under conditions of hypercholesterolemia. PMID:24284391

  5. Study on the salted asparagus food%盐渍芦笋食品的研究

    Institute of Scientific and Technical Information of China (English)

    刘娜丽; 韩晓静; 杨红; 李久长

    2012-01-01

    In this paper, use asparagus as raw materials, the research on salted asparagus food, as spicy asparagus and pickled asparagus. After a lot of experiments,we have decided the suitable processing technology and the best prescription, and then the main physical and chemical properties of the asparagus production were determined.%利用新鲜芦笋为原料,进行盐渍芦笋食品芦笋泡菜、麻辣芦笋的研制,通过试验,确定配方及生产工艺,并对成品的理化指标进行测定。

  6. Patterns of spatial and temporal distribution of the asparagus miner (Diptera: Agromyzidae): implications for management.

    Science.gov (United States)

    Morrison, William R; Szendrei, Zsofia

    2013-06-01

    The asparagus miner is an obligatory feeder on asparagus and a putative vector for pathogenic fungi implicated in the early decline of asparagus fields. To date, the distribution of the asparagus miner over space and time is poorly understood. Our study evaluated the spatial and temporal pattern of adult asparagus miners in commercial asparagus fields in Michigan in 2011 and 2012. We sampled adults and damage weekly during the growing season using yellow sticky traps outside, at the edge, and inside commercial fields. Yellow sticky traps at each trapping location were placed at the canopy and ground level to determine vertical distribution of adults. During the first generation, adults were more evenly distributed throughout the field. In the second generation, adults were more commonly found on the edge of the field. Overall, there was a greater percent of mining damage near the edge of the field. Additionally, three times as many asparagus miners were found in the canopy compared with ground-level traps. There were 12 times as many asparagus miner adults on edges bordered by another asparagus field than on ones bordered by forest. Taken together, our results indicate that while asparagus miner management in the beginning of the growing season should focus on the entire field, in the latter half of the season, growers could save money and resources by targeting miner adults at the edges of fields. Finally, conserving the remaining naturally forested landscape and planting borders of trees may help ameliorate pest pressure in asparagus fields.

  7. Asparagus byproducts as a new source of peroxidases.

    Science.gov (United States)

    Jaramillo-Carmona, Sara; Lopez, Sergio; Vazquez-Castilla, Sara; Rodriguez-Arcos, Rocio; Jimenez-Araujo, Ana; Guillen-Bejarano, Rafael

    2013-07-01

    Soluble peroxidase (POD) from asparagus byproducts was purified by ion exchange chromatographies, and its kinetic and catalytic properties were studied. The isoelectric point of the purified isoperoxidases was 9.1, and the optimum pH and temperature values were 4.0 and 25 °C, respectively. The cationic asparagus POD (CAP) midpoint inactivation temperature was 57 °C, which favors its use in industrial processes. The Km values of cationic asparagus POD for H₂O₂ and ABTS were 0.318 and 0.634 mM, respectively. The purified CAP is economically obtained from raw materials using a simple protocol and possesses features that make it advantageous for the potential use of this enzyme in a large number of processes with demonstrated requirements of thermostable POD. The results indicate that CAP can be used as a potential candidate for removing phenolic contaminants.

  8. Irradiation degradation of chlorpyrifos in water solution and asparagus

    International Nuclear Information System (INIS)

    In order to seek an effective technique to degrade chlorpyrifos residue, chlorpyrifos water solution and asparagus containing chlorpyrifos as testing materials were irradiated by 60Co γ-rays and their degradation rate were determined and compared. The results show the degradation rate in water-solution increases with irradiation dose in the range of 0 and 12 kGy, and it reached 95.5% at 4 kGy. The degradation rate of chlorpyrifos in asparagus is low and comes to the maximum of 30.0% when the dose is 8 kGy. Further study indicates that vitamin C, violaquercitrin and total sugar inhibit the irradiation degradation of chlorpyrifos in asparagus. (authors)

  9. Improvement of crop technology in Asparagus plumosus Bak. A

    Directory of Open Access Journals (Sweden)

    Dumitru ZAHARIA

    1997-08-01

    Full Text Available Improvement of crop technology in Asparagus plumosus Bak. A 0,1% nutritive solution of the N, P, K, elements in raport of 3:1:2; 4:2:3 and 4:3:3 respectively was used to fertilise the plants Asparagus plumosus Bak., for the period between February and Octomber. A 29,8 to 35,20 posesive semnificative increase production of the shoot number per m2 was obtained. The quality of the shoots was not semnificatively influenced by the applied fertilisation system but it appeared to be determined by the cultural factors, light and temperature.

  10. 超声波辅助提取芦笋总皂苷的工艺%Use of Response Surface Methodology for Optimization of Ultrasound-assisted Extraction of Total Saponins from Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张若洁; 王鲁峰; 缪文华; 徐晓云; 潘思轶

    2011-01-01

    采用Box—Behnken设计和响应面分析法,对芦笋总皂苷的超声辅助提取工艺进行优化。以提取温度、提取时间及超声功率为自变量,芦笋总皂苷得率为响应值,研究各自变量及其交互作用对超声波辅助提取法提取芦笋总皂苷得率的影响。结果表明:最佳工艺条件为乙醇体积分数95%、提取温度71℃、提取时间23min、超声功率70W、提取次数2次,在此条件下获得总皂苷的提取率为23.96mg/g。%Response surface methodology based on a three-variable, three-level Box-Behnken experimental design was used to optimize ultrasound-assisted extraction of total saponins from Asparagus officinalis L. The extraction yield of total saponins was investigated with respect to temperature, extraction time and ultrasonic power. The optimal extraction conditions were found to be: ethanol concentration 95%, temperature 71 ℃, extraction time 23 min, ultrasound power 70 W, and number of extractions 2. Under these conditions, the extraction yield of total saponins was 23.96 mg/g.

  11. 芦笋叶绿素铜钠盐制备过程中提取工艺的研究%Study on extraction process of chlorophyll and preparation of sodium copper chlorophyllin from Asparagus officinalis leaves

    Institute of Scientific and Technical Information of China (English)

    赵庆生; 孙健; 肖杰; 赵兵; 黄云祥

    2012-01-01

    The extraction process of chlorophyll and the preparation of sodium copper chlorophyllin from leaves of Asparagus officinalis were studied.The results showed that the extraction solvent was 80% acetone and 95% ethanol with the ratio of 1:4,the ratio of liquid to solid was 10:1,the extraction temperature was 70℃,the extracting time was 60min and ultrasonic power was 160W.Then sodium copper chlorophyllin was made by chemical progressing and the yield was 0.18%.The quality of sodium copper cholorphyllin met the national standard GB 3262-1982.%采用超声法对芦笋叶进行了叶绿素的提取并合成了叶绿素铜钠盐。结果表明,芦笋叶绿素的最佳提取工艺条件为:提取溶剂为80%丙酮与95%乙醇体积比1:4,提取时间60min,提取温度70℃,液固比10:1,超声功率160W。提取的叶绿素溶液,经过皂化、酸化、铜代、成盐等步骤制成叶绿素铜钠盐,得率(以鲜叶计)为0.18%,产品质量符合国家标准GB3262-1982。

  12. 芦笋组织培养中玻璃化苗逆转的初步研究%Preliminary Study on Reversion of Vitrification in Tissue Culture of Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    张天翔; 林宗铿; 蔡坤秀; 杨俊杰; 曹明华

    2013-01-01

    为探讨芦笋(Asparagus officinalis L.)玻璃化试管苗的逆转恢复途径,对培养温度、光照强度、蔗糖浓度、琼脂浓度等因素对玻璃化苗逆转效果的影响进行研究.结果显示:玻璃化苗的恢复率随着培养温度的降低而有所升高,培养温度为15℃时,恢复率为33.01%;玻璃化苗的恢复率随光照强度的增强而明显升高,当光照强度为5200lx时,逆转率可达70.55%;玻璃化苗的恢复率随着蔗糖浓度的升高而逐渐升高,当蔗糖浓度为50g/L时,恢复率可达48.20%;玻璃化苗的恢复率随琼脂浓度的增加而升高,琼脂浓度为9g/L时,恢复率达38.18%.说明在4种影响因素中,光照强度对玻璃化苗逆转的影响最大,而培养温度、蔗糖浓度、琼脂浓度对玻璃化苗的逆转也有一定的影响.

  13. Producción de Bacillus thuringiensis H-14 var. Israelensis utilizando espárrago (Asparagus officinalis y su uso potencial para el control de la Malaria en la Libertad- Perú

    Directory of Open Access Journals (Sweden)

    Franklin Vargas V

    2001-07-01

    Full Text Available Objetivo: Estandarizar un medio, de cultivo utilizando la infusión de espárrago (Asparagus officinalis para la producción masiva de Bacillus thuringiensis (Bti H-14 var. israelensis y determinar el efecto biolarvicida del Bti sobre Anopheles sp en criaderos naturales del distrito Laredo durante los meses de Enero a Diciembre del 2000. Materiales y métodos: Se ensayaron 3 medios a base de infusión de espárrago blanco: M1: 100 mL de la infusión, pH 9; M2: 50 mL de la infusión con 50 mL de buffer fosfato, pH 7; y M3: 25 mL de la infusión con 75 mL de agua destilada, pH 9. Como control se utilizó el medio estándar TPH. La producción de Bti en los medios de cultivo fueron evaluadas para determinar la efectividad biolarvicida a través del LC50 y LC90. El medio de cultivo óptimo (menor LC50 y LC90 sirvió para la producción masiva del Bti, el cual se sometió a bioensayos de laboratorio y aplicaciones en criaderos naturales. La efectividad fue determinada mediante la densidad larvaria pre y post aplicación del Bti. Resultados: El medio de cultivo óptimo para la producción de Bti fue M1 mostrando alta efectividad, con 100% de mortalidad en condiciones de laboratorio y 71-97% de mortalidad en el campo a las 24 horas de exposición con 3 aplicaciones realizadas semanalmente. Conclusiones: M1 es el medio optimo para cultivar Bti, con alta efectividad para controlar larvas de Anopheles en el laboratorio y en el campo.

  14. Early selection of elite plants in Asparagus

    Directory of Open Access Journals (Sweden)

    COINTRY ENRIQUE LUIS

    2000-01-01

    Full Text Available In order to establish an efficient selection criterion the variability in three asparagus populations was evaluated defining the most important yield components and analysing its evolution along three growing seasons. The yield components, coefficient of variation (CV and the proportion of plants contributing to 80% of the total yield were estimated. The elite plants were selected by mean of total yield and clusters techniques. Multiple regression showed that spear number (SN and spear weight (SW were the most important yield components. In every population, total yield (TY and SN showed the highest values of CV, independently of sex. 69% of the plants contributed to the 80% of the total yield in the first year while in the second and third year the contribution was 57%. At the end of the third year, 17 plants were selected by the average of the total yield and 43 by clusters. It is suggested to select for SW in the first year, reducing in 68% the experimental material. In the second year, the selection for SN would reduce to 5% the plants to evaluate for total yield in the third year. In this way the selected plants are the same but the number of plants to evaluate is dramatically reduced along the years, therefore facilitating the breeders work.

  15. Changes in communities of Fusarium and arbuscular mycorrhizal fungi as related to different asparagus cultural factors.

    Science.gov (United States)

    Yergeau, Etienne; Vujanovic, Vladimir; St-Arnaud, Marc

    2006-07-01

    Asparagus (Asparagus officinalis) is a high-value perennial vegetable crop that has shown a marked decline in productivity after many years of continuous harvesting. This decline is caused by an increase in both abiotic (autotoxicity, harvesting pressure) and biotic stresses [fungal infections, mainly Fusarium crown and root rot (FCRR)]. To gain insight into disease development and possible mitigation strategies, we studied the effects of harvesting, time in the growing season, and field age on FCRR development, Fusarium species composition, and arbuscular mycorrhizal fungi (AMF) communities in both a controlled field experiment and an ecological survey of commercial fields. In one experiment, a 3-year-old asparagus field was subdivided into plots that were harvested or not and sampled throughout the growing season to assess short-term dominant Fusarium species shifts. In addition, diseased and healthy asparagus plants sampled from six commercial fields in the same geographical region were used to assess Fusarium and AMF communities in relation to different parameters. Fusarium and AMF communities were described by using a polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) approach, and results were analyzed by mainly correspondence analysis and canonical correspondence analysis. Results showed that dominant Fusarium taxa assemblages changed throughout the growing season. Harvested plots had significantly more FCRR symptomatic plants at the end of the growing season, but this effect was not related with any trend in Fusarium community structure. Sampling site and plant age significantly influenced AMF community structure, whereas only sampling site consistently influenced the Fusarium community. Diseased and healthy plants harbored similar Fusarium and AMF communities. Shifts in Fusarium community might not be responsible for different disease incidence because they are ubiquitous regardless of plant health status or harvesting regime

  16. Competitiveness and Prospects of Greek Asparagus: The European Context

    Directory of Open Access Journals (Sweden)

    Argyrios D. Kolokontes

    2009-01-01

    Full Text Available Problem statement: The revisions and the anticipated abolition of CAP will change the agricultural structure in member states of EU. Although the asparagus, without government interventions, from the decade of 90' has been transformed at one of more promising, in exports terms, cultivation for Greece, however under the existing circumstances it has a doubtful future. Approach: This research studied the diachronic evolution of competitiveness of white fresh Greek asparagus into the European context. A scheme of trade indices, based on post-trade and post-production data, had been estimated in respect of absence from the literature of a single generally accepted measure of competitiveness. Results: The results demonstrated that the Greek asparagus presents both a highly dependency of the German market and a penetration weakness into other markets, except from the Dutch one. The lack of Greek large retail chains with considerable geographic dispersion in West Europe contributes to trading problem. The feeble domestic consumption in Greece in combination with the enlargement of German production and the implied diminishing of its imports, cause restlessness for the future of cultivation and the producers'income in Greece. Conclusion: A long term trading pattern for bonds creation in foreign supply channels and the growth of domestic consumption in Greece are indispensable to support and expand the future prospects of Greek asparagus.

  17. Retrotransposon Proliferation Coincident with the Evolution of Dioecy in Asparagus

    Science.gov (United States)

    Harkess, Alex; Mercati, Francesco; Abbate, Loredana; McKain, Michael; Pires, J. Chris; Sala, Tea; Sunseri, Francesco; Falavigna, Agostino; Leebens-Mack, Jim

    2016-01-01

    Current phylogenetic sampling reveals that dioecy and an XY sex chromosome pair evolved once, or possibly twice, in the genus Asparagus. Although there appear to be some lineage-specific polyploidization events, the base chromosome number of 2n = 2× = 20 is relatively conserved across the Asparagus genus. Regardless, dioecious species tend to have larger genomes than hermaphroditic species. Here, we test whether this genome size expansion in dioecious species is related to a polyploidization and subsequent chromosome fusion, or to retrotransposon proliferation in dioecious species. We first estimate genome sizes, or use published values, for four hermaphrodites and four dioecious species distributed across the phylogeny, and show that dioecious species typically have larger genomes than hermaphroditic species. Utilizing a phylogenomic approach, we find no evidence for ancient polyploidization contributing to increased genome sizes of sampled dioecious species. We do find support for an ancient whole genome duplication (WGD) event predating the diversification of the Asparagus genus. Repetitive DNA content of the four hermaphroditic and four dioecious species was characterized based on randomly sampled whole genome shotgun sequencing, and common elements were annotated. Across our broad phylogenetic sampling, Ty-1 Copia retroelements, in particular, have undergone a marked proliferation in dioecious species. In the absence of a detectable WGD event, retrotransposon proliferation is the most likely explanation for the precipitous increase in genome size in dioecious Asparagus species. PMID:27342737

  18. 76 FR 6313 - Asparagus Revenue Market Loss Assistance Payment Program

    Science.gov (United States)

    2011-02-04

    ... available funding for each marketing category. CCC published a proposed rule on July 16, 2010 (75 FR 41397... forth in the Notice to 7 CFR part 3015, subpart V (48 FR 29115, June 24, 1983), the programs and... Commodity Credit Corporation 7 CFR Part 1429 RIN 0560-AI02 Asparagus Revenue Market Loss Assistance...

  19. 75 FR 41397 - Asparagus Revenue Market Loss Assistance Payment Program

    Science.gov (United States)

    2010-07-16

    ... Secretary of Agriculture published in the Federal Register on July 24, 1971 (36 FR 13804), a Statement of... prepared. Executive Order 12372 For reasons set forth in the Notice to 7 CFR part 3015, subpart V (48 FR... Commodity Credit Corporation 7 CFR Part 1429 RIN 0560-AI02 Asparagus Revenue Market Loss Assistance...

  20. Phytochemical Screening and Aphrodisiac Activity of Asparagus racemosus

    Directory of Open Access Journals (Sweden)

    Javeed Ahmed Wani

    2011-04-01

    Full Text Available The plant Asparagus racemosus is widely distributed in the Himalayan and sub-Himalayan regions of India. Based on preliminary reports, there is a lot of interest in using the roots of this plant for treating sexual disorders. In this study, the hydro-alcoholic and aqueous extracts of the roots of Asparagus racemosus were subjected to preliminary phytochemical screening which showed the presence of saponins, carbohydrates, glycosides and mucilages. The total extracts were tested for their aphrodisiac activity in experimental rats. The hydro-alcoholic extract of Asparagus racemosus root at higher concentration (400 mg/kg body weight showed significant aphrodisiac activity on male wistar albino rats as evidenced by an increase in number of mounts and mating performance. On the other hand, hydro-alcoholic extract at lower dose (200 mg/kg. body weight and aqueous extract (400 mg/kg body weight showed moderate aphrodisiac property. Thus, in experimental rats, the results of the present study suggest that the extracts of Asparagus racemosus exert significant aphrodisiac activity. Further, detailed studies are needed to know whether in-vivo administration of the extracts is beneficial for patients suffering from sexual disorders.

  1. Antiprotozoal compounds from Asparagus africanus

    DEFF Research Database (Denmark)

    Oketch-Rabah, H A; Dossaji, S F; Christensen, S B;

    1997-01-01

    Two antiprotozoal compounds have been isolated from the roots of Asparagus africanus Lam. (Liliaceae), a new sapogenin, 2 beta, 12 alpha-dihydroxy-(25R)-spirosta-4,7-dien-3-one (1), which was named muzanzagenin, and the lignan (+)-nyasol (2), (Z)-(+)-4,4'-(3-ethenyl-1-propene-1,3-diyl)-bisphenol...

  2. Retrotransposon Proliferation Coincident with the Evolution of Dioecy in Asparagus

    Directory of Open Access Journals (Sweden)

    Alex Harkess

    2016-09-01

    Full Text Available Current phylogenetic sampling reveals that dioecy and an XY sex chromosome pair evolved once, or possibly twice, in the genus Asparagus. Although there appear to be some lineage-specific polyploidization events, the base chromosome number of 2n = 2× = 20 is relatively conserved across the Asparagus genus. Regardless, dioecious species tend to have larger genomes than hermaphroditic species. Here, we test whether this genome size expansion in dioecious species is related to a polyploidization and subsequent chromosome fusion, or to retrotransposon proliferation in dioecious species. We first estimate genome sizes, or use published values, for four hermaphrodites and four dioecious species distributed across the phylogeny, and show that dioecious species typically have larger genomes than hermaphroditic species. Utilizing a phylogenomic approach, we find no evidence for ancient polyploidization contributing to increased genome sizes of sampled dioecious species. We do find support for an ancient whole genome duplication (WGD event predating the diversification of the Asparagus genus. Repetitive DNA content of the four hermaphroditic and four dioecious species was characterized based on randomly sampled whole genome shotgun sequencing, and common elements were annotated. Across our broad phylogenetic sampling, Ty-1 Copia retroelements, in particular, have undergone a marked proliferation in dioecious species. In the absence of a detectable WGD event, retrotransposon proliferation is the most likely explanation for the precipitous increase in genome size in dioecious Asparagus species.

  3. Retrotransposon Proliferation Coincident with the Evolution of Dioecy in Asparagus.

    Science.gov (United States)

    Harkess, Alex; Mercati, Francesco; Abbate, Loredana; McKain, Michael; Pires, J Chris; Sala, Tea; Sunseri, Francesco; Falavigna, Agostino; Leebens-Mack, Jim

    2016-01-01

    Current phylogenetic sampling reveals that dioecy and an XY sex chromosome pair evolved once, or possibly twice, in the genus Asparagus Although there appear to be some lineage-specific polyploidization events, the base chromosome number of 2n = 2× = 20 is relatively conserved across the Asparagus genus. Regardless, dioecious species tend to have larger genomes than hermaphroditic species. Here, we test whether this genome size expansion in dioecious species is related to a polyploidization and subsequent chromosome fusion, or to retrotransposon proliferation in dioecious species. We first estimate genome sizes, or use published values, for four hermaphrodites and four dioecious species distributed across the phylogeny, and show that dioecious species typically have larger genomes than hermaphroditic species. Utilizing a phylogenomic approach, we find no evidence for ancient polyploidization contributing to increased genome sizes of sampled dioecious species. We do find support for an ancient whole genome duplication (WGD) event predating the diversification of the Asparagus genus. Repetitive DNA content of the four hermaphroditic and four dioecious species was characterized based on randomly sampled whole genome shotgun sequencing, and common elements were annotated. Across our broad phylogenetic sampling, Ty-1 Copia retroelements, in particular, have undergone a marked proliferation in dioecious species. In the absence of a detectable WGD event, retrotransposon proliferation is the most likely explanation for the precipitous increase in genome size in dioecious Asparagus species. PMID:27342737

  4. 青贮添加剂对芦笋茎叶青贮品质的影响%Effect of additives on the quality of Asparagus officinalis stem leaf silage

    Institute of Scientific and Technical Information of China (English)

    郭海明; 朱雯; 张勇; 黄文明; 焦阳; 叶均安

    2016-01-01

    本研究旨在探讨不同添加剂对青贮芦笋茎叶品质的影响。试验在芦笋茎叶鲜样中分别添加乳酸菌、乳酸菌+纤维素酶、乳酸菌+米糠和乳酸菌+纤维素酶+米糠进行青贮调制,乳酸菌、纤维素酶和米糠的添加剂量分别为5×105 cfu/g、0.6 IU/g、30 mg/g,贮存90 d 后开包。综合评定芦笋茎叶的青贮质量,测定常规营养成分,浸提液的 pH 值、氨态氮和有机酸的含量。试验结果表明,与对照组相比,添加乳酸菌提高了乙酸的含量、降低了有氧损失(P <0.05),提高了芦笋青贮料的有氧稳定性;在添加乳酸菌基础上,同时添加米糠和纤维素酶有降低青贮品质的趋势。仅添加乳酸菌即可获得优质芦笋茎叶青贮料。%The objective of this study was to investigate the effects of different additives on the quality of Asparagus officinalis silage (stem and leaf).Lactobacillus,lactobacillus+cellulose,lactobacillus+rice bran and lactobacillus+cellulose+rice bran were added to silage;the concentration of lactobacillus,cellulose and rice bran were 5×10 5 cfu/g,0.6 IU/g,30 mg/g of fresh weight respectively.After being ensiled for 90 days, the quality of A.officinalis stem leaf silage was assessed.The chemical composition,pH,NH 3-N and organic acid content of fermented juice were analysed.The data showed that,compared with the control group,the ad-dition of lactobacillus increased the acetic acid content,reduced aerobic deterioration (P <0.05)and improved the aerobic stability.Lactobacillus with rice bran and cellulose tended to lower the quality of A.officinalis stem leaf silage.The results demonstrated that high quality A.officinalis stem leaf silage can be obtained with addition of lactobacillus only.

  5. Primary investigation of infection diseases in asparagus in Henan province%河南省芦笋侵染性病害调查初报

    Institute of Scientific and Technical Information of China (English)

    康业斌; 郭岩; 王进涛

    2000-01-01

    @@ 芦笋(Asparagus officinalis L.),又名石刁柏,是百合科天门冬属多年生草本植物,其嫩茎作为蔬菜和特殊医疗用品,可加工成罐头或直接速冻出口,为国际市场上的畅销品.

  6. New combinations and a complete list of Asparagus species in southern Africa (Asparagaceae

    Directory of Open Access Journals (Sweden)

    A. C. Fellingham

    1995-12-01

    Full Text Available With only flower sexuality differing. Protasparagus can not be separated from  Asparagus, neither at generic nor at subgeneric level. No significant differences in the degree to which filaments were connivent were observed between species placed under  Myrsiphyllum and those under  Protasparagus/Asparagus. All southern African species of Asparagaceae are therefore reinstated under Asparagus and listed here without recognition of subgenera. Several new combinations are made.

  7. Comparison of Proximate and Mineral composition between Asparagus Oficinalis and Momordica dioica: Iranian and Indian Vegetables

    Directory of Open Access Journals (Sweden)

    Ali Aberoumand

    2010-06-01

    Full Text Available The proximate composition and mineral constituents of Asparagus officinalis stem and Momordica dioica fruit were evaluated in order to standard methods. The stem contained a ashes: 10.70%,crude protein: 32.69%, crude lipid: 3.44%, crude fiber: 18.50%, and carbohydrates: 34.67%. Stem also have high energy value (384.27kcal/100gdry weight. Mineral ranges (mg/100g dry weight, DW were: K (10.94, Na (1.84, Ca (0.67, Fe (0.19, and Zn (2.60. The fruits contained a ashes: 9.1%, crude protein: 5.44%, crude lipid: 3.25%, crude fiber: 22.9%, and carbohydrates: 59.31%. The fruits also have high energy value (288.25kcal/100gdry weight. Mineral ranges (mg/100g dry weight, DW were: K (4.63, Na (1.62, Ca (7.37, Fe (5.04, and Zn (3.83. Comparing proximate and minerals contents of the stem and the fruit, the results indicated that Asparagus officinalis stem could be a good supplement for some nutrients such as protein, lipid, potassium and Zinc, fibre and carbohydrates while Momordica dioica fruit was good source of lipid, crude fiber, carbohydrates, Fe and Zinc.

  8. Skill Acquisition in "High Tech" Export Agriculture: A Case Study of Lifelong Learning in Peru's Asparagus Industry

    Science.gov (United States)

    Carnoy, Martin; Luschei, Thomas F.

    2008-01-01

    As one of the world's largest exporters of asparagus, Peru has developed a high-tech system of asparagus production, processing and delivery that requires well-trained and responsive workers. In this study we examine the role of both private and public sectors in preparing workers for the asparagus industry and the implications of this skill…

  9. Stress Physiology and Virulence Characterization of Phomopsis asparagi (Sacc.) Bub6k isolated from Asparagus in Jiangxi Province of China

    Institute of Scientific and Technical Information of China (English)

    Yueping ZHANG; Guangyu CHEN; Shaochun LUO; Huaxiang QU; Yongping TANG; Qixin XIE; Jinsong ZHOU

    2012-01-01

    Abstract Fungal pathogen of asparagus stem blight was isolated. No significant ge- netic difference was detected among the three strains with 492 bp long ITS1-5.8S- ITS2 sequence. It was then identified through colony growth, conidia morphology, and molecular characterization. The physiological response to oxidation and osmosis stress, and virulence to Asparagus officinalis L. were analyzed. The results showed that the pathogen causing asparagus stem blight for A. officinalis L. in Jiangxi Province is Phomopsis asparagri (Sacc.) Bubok. Under pure culture conditions, the conidia were oval-shaped (a-type), with colorless single spore and single nucleus, containing 0-2 oil balls. Its vegetative growth rate was higher when cultured on 0.2 x potato dextrose agar (0.2 x PDA) medium than that on oatmeal agar (OA) medium. However, the pycnidia appeared earlier on OA medium than on 0.2 earlier PDA medium. The vegetative growth rate was depressed under oxidation (H202) or osmo- sis (NaCI) stress conditions, and totally inhibited under 7 mmol/L H202 or 2.4 mol/L NaCI. All the strains caused typical pathogenic symptoms to Asparagus officinalis L. at 7 days-post-inoculation (dpi) with conidia.

  10. Immunotherapeutic modulation of intraperitoneal adhesions by Asparagus racemosus.

    Directory of Open Access Journals (Sweden)

    Rege N

    1989-10-01

    Full Text Available The hypothesis that macrophages appear to play a pivotal role in the development of intraperitoneal adhesions and that modulation of macrophage activity, therefore, is likely to provide a tool for prevention of adhesions, was tested in the present study. Effect of Asparagus racemosus, an indigenous agent with immunostimulant properties, was evaluated in an animal model of intraperitoneal adhesions induced by caecal rubbing. Animals were sacrificed 15 days following surgery. The peritoneal macrophages were collected to assess their activity. At the same time, peritoneal cavity was examined for the presence of adhesions, which were graded. A significant decrease was observed in the adhesion scores attained by animals receiving Asparagus racemosus. This was associated with significant increase in the activity of macrophages (70.1 +/- 2.52, compared to that in surgical controls (53.77 +/- 10.8. These findings support our hypothesis and provide a novel approach for the prevention and management of post-operative adhesions.

  11. Cell wall bound anionic peroxidases from asparagus byproducts.

    Science.gov (United States)

    Jaramillo-Carmona, Sara; López, Sergio; Vazquez-Castilla, Sara; Jimenez-Araujo, Ana; Rodriguez-Arcos, Rocio; Guillen-Bejarano, Rafael

    2014-10-01

    Asparagus byproducts are a good source of cationic soluble peroxidases (CAP) useful for the bioremediation of phenol-contaminated wastewaters. In this study, cell wall bound peroxidases (POD) from the same byproducts have been purified and characterized. The covalent forms of POD represent >90% of the total cell wall bound POD. Isoelectric focusing showed that whereas the covalent fraction is constituted primarily by anionic isoenzymes, the ionic fraction is a mixture of anionic, neutral, and cationic isoenzymes. Covalently bound peroxidases were purified by means of ion exchange chromatography and affinity chromatography. In vitro detoxification studies showed that although CAP are more effective for the removal of 4-CP and 2,4-DCP, anionic asparagus peroxidase (AAP) is a better option for the removal of hydroxytyrosol (HT), the main phenol present in olive mill wastewaters.

  12. HPLC法同时测定芦笋黄酮中5个黄酮苷的含量%HPLC simultaneous determination of five flavonoid glycosides in Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    姜云云; 叶光明; 范国荣; 陈云红

    2012-01-01

    目的:建立反相高效液相色谱法同时测定芦笋黄酮中槲皮素-3-O-葡萄糖-芸香糖苷、芦丁、异鼠李素-3 -O-葡萄糖-芸香糖苷、烟花苷和水仙苷的含量.方法:采用Diamonsil(钻石)C18柱(200 mm ×4.6 mm,5 μm),流动相为乙腈-甲醇-0.1%醋酸水(8∶16∶76),流速1.0mL·min-1,柱温30℃,检测波长260 nm.结果:芦笋黄酮中槲皮素-3-O-葡萄糖-芸香糖苷、芦丁、异鼠李素-3-O-葡萄糖-芸香糖苷、烟花苷和水仙苷浓度分别在30.38~70.88μg·mL-1(r=0.9996)、60.75 ~141.75μ·mL-1(r=0.9995)、29.40 ~68.60 μg· mL-1(r =0.9997)、29.63~69.13 μg·mL-1 (r=0.9996) 、18.00~42.00 μg·mL-1(r =0.9998)范围内与峰面积呈良好的线性关系.方法的加样回收率(n=6)分别为99.4%,99.2%,99.2%,102.6%,101.4%;RSD分别为2.0%,1.6%,1.8%,1.3%,2.2%.结论:本法快速、简便、准确,重复性好,适用于芦笋黄酮的质量控制.%Objective:To develop an HPLC method for simultaneous determining quercetin - 3 - 0 - glucosyl - ru-tinoside,rutin,isorhamnetin -3 - 0 - glucosyl - rutinoside, nicotiflorin and narcissin in total flavonoids of Asparagus officinalis L. Methods;The separation was carried out on a Diamonsil CI8(200 mm×4. 6 mm,5μm)column. The mobile phase consisted of acetonitrile -methanol -0. 1% acetic acid(8:16:76) at a flow rate of 1.0 mLo min-1. The detection wavelength was set at 260 nm and the column temperature was 30℃. Results;The linear ranges of quercetin - 3 - 0 - glucosyl - rutinoside, rutin, isorhamnetin - 3 - 0 - glucosyl - rutinoside, nicotiflorin and narcissin were 30. 38 - 70. 88 μg·mL-1(γ =0.9996),60.75-141.75 μg o mL-1 (r= 0.9995) ,29.40-68.60 μg o mL-1(r=0.9997),29.63-69. 13 μg o mL-1(r =0. 9996)and 18.00-42.00 μg o mL-1(r=0.9998) ,respectively . The average recoveries (n- 6) of quercetin - 3-0- glucosyl - rutinoside, rutin, isorhamnetin - 3 - 0 - glucosyl - rutinoside, nicotiflorin and narcissin were 99. 4% , 99. 2% , 99. 2

  13. Genetic and physical maps around the sex-determining M-locus of the dioecious plant asparagus.

    Science.gov (United States)

    Telgmann-Rauber, Alexa; Jamsari, Ari; Kinney, Michael S; Pires, J Chris; Jung, Christian

    2007-09-01

    Asparagus officinalis L. is a dioecious plant. A region called the M-locus located on a pair of homomorphic sex chromosomes controls the sexual dimorphism in asparagus. The aim of this work was to clone the region determining sex in asparagus from its position in the genome. The structure of the region encompassing M should be investigated and compared to the sex-determining regions in other dioecious model species. To establish an improved basis for physical mapping, a high-resolution genetic map was enriched with AFLP markers closely linked to the target locus by carrying out a bulked segregant analysis. By screening a BAC library with AFLP- and STS-markers followed by chromosome walking, a physical map with eight contigs could be established. However, the gaps between the contigs could not be closed due to a plethora of repetitive elements. Surprisingly, two of the contigs on one side of the M-locus did not overlap although they have been established with two markers, which mapped in a distance as low as 0.25 cM flanking the sex locus. Thus, the clustering of the markers indicates a reduced recombination frequency within the M-region. On the opposite side of the M-locus, a contig was mapped in a distance of 0.38 cM. Four closely linked BAC clones were partially sequenced and 64 putative ORFs were identified. Interestingly, only 25% of the ORFs showed sequence similarity to known proteins and ESTs. In addition, an accumulation of repetitive sequences and a low gene density was revealed in the sex-determining region of asparagus. Molecular cytogenetic and sequence analysis of BACs flanking the M-locus indicate that the BACs contain highly repetitive sequences that localize to centromeric and pericentromeric locations on all asparagus chromosomes, which hindered the localization of the M-locus to the single pair of sex chromosomes. We speculate that dioecious Silene, papaya and Asparagus species may represent three stages in the evolution of XX, XY sex

  14. Genetic and physical maps around the sex-determining M-locus of the dioecious plant asparagus.

    Science.gov (United States)

    Telgmann-Rauber, Alexa; Jamsari, Ari; Kinney, Michael S; Pires, J Chris; Jung, Christian

    2007-09-01

    Asparagus officinalis L. is a dioecious plant. A region called the M-locus located on a pair of homomorphic sex chromosomes controls the sexual dimorphism in asparagus. The aim of this work was to clone the region determining sex in asparagus from its position in the genome. The structure of the region encompassing M should be investigated and compared to the sex-determining regions in other dioecious model species. To establish an improved basis for physical mapping, a high-resolution genetic map was enriched with AFLP markers closely linked to the target locus by carrying out a bulked segregant analysis. By screening a BAC library with AFLP- and STS-markers followed by chromosome walking, a physical map with eight contigs could be established. However, the gaps between the contigs could not be closed due to a plethora of repetitive elements. Surprisingly, two of the contigs on one side of the M-locus did not overlap although they have been established with two markers, which mapped in a distance as low as 0.25 cM flanking the sex locus. Thus, the clustering of the markers indicates a reduced recombination frequency within the M-region. On the opposite side of the M-locus, a contig was mapped in a distance of 0.38 cM. Four closely linked BAC clones were partially sequenced and 64 putative ORFs were identified. Interestingly, only 25% of the ORFs showed sequence similarity to known proteins and ESTs. In addition, an accumulation of repetitive sequences and a low gene density was revealed in the sex-determining region of asparagus. Molecular cytogenetic and sequence analysis of BACs flanking the M-locus indicate that the BACs contain highly repetitive sequences that localize to centromeric and pericentromeric locations on all asparagus chromosomes, which hindered the localization of the M-locus to the single pair of sex chromosomes. We speculate that dioecious Silene, papaya and Asparagus species may represent three stages in the evolution of XX, XY sex

  15. Establishment of a resistance-identification method on asparagus stem blight and evaluation of Asparagus officinalis germplasms%芦笋茎枯病抗性鉴定方法的建立及芦笋抗病种质资源的筛选

    Institute of Scientific and Technical Information of China (English)

    杨迎青; 李湘民; 孟凡; 兰波

    2012-01-01

    Asparagus stem blight is a worldwide devastating disease and the screening of resistant varieties is the most economical and effective control method. However, there hasn' t been a simple, fast and effective resistance-identification method. Four inoculation methods at the seedling stage, i. e. the mycelial pellet sticking on the base of stems, on the middle of stems, both on the bases and the middle of stems and the spore-spraying method, were firstly compared, with JK701 and TX-4/SD as the material. The result revealed that the spore-spraying method was easier for operation, faster for causing diseases symptoms and more convenient to divide the disease grades, while other three methods could only cause light symptoms. Then the spore-spraying method was optimized in two aspects, including isolate virulence and sprayed concentrations of spore suspension. The results showed that the isolate FJ2 behaved the strongest virulence and was used for resistance identification , 1 × 106 spore/mL was the best inoculation concentration. To verify the accuracy of the spore-spraying method, 5 varieties with different resistant levels were tested both at the seedling and the adult phase and the identification results of the seedling phase were almost consistent to those of the adult phase. Finally, 31 resources were carried out resistant identification using the established spore-spraying method. The results revealed that the disease indexes of the wild asparagus were 0, which was identified as a immunity germplasm resource, the disease indexes of TX4/SD、Shuofeng and UC157 were slow, which were identified as resistant germplasm resources, while others were susceptible.

  16. The potential of Asparagus-P to inactivate reactive oxygen radicals.

    Science.gov (United States)

    Dartsch, Peter C

    2008-02-01

    Asparagus-P is a traditional herbal medicinal product consisting of a combination of asparagus roots and parsley leaves in equal shares. It is used to support aquaretic kidney function. The present study was undertaken to shed light on the antioxidative effects of both main ingredients and their combination as in Asparagus-P. For that purpose, differentiated promyelocytic HL60 cells (functional neutrophils) capable of generating superoxide radicals upon stimulation, were used. Parsley leaves and, hence, also Asparagus-P had a marked dose-dependent antioxidant effect, whereas asparagus roots had only a minor efficacy in this respect. In conclusion, Asparagus-P does not only support kidney function, but is also able to inactivate reactive oxygen radicals which might occur by a metabolic or exogenous overload (oxidative stress) or in the course of inflammatory processes. This antioxidant efficacy is primarily related to the content of parsley leaves; asparagus roots seem to enhance this efficacy by promoting the potential of parsley leaves, thus generating a synergistic effect in the combination product Asparagus-P.

  17. Optimization of a method for the profiling and quantification of saponins in different green asparagus genotypes.

    Science.gov (United States)

    Vázquez-Castilla, Sara; Jaramillo-Carmona, Sara; Fuentes-Alventosa, Jose María; Jiménez-Araujo, Ana; Rodriguez-Arcos, Rocío; Cermeño-Sacristán, Pedro; Espejo-Calvo, Juan Antonio; Guillén-Bejarano, Rafael

    2013-07-01

    The main goal of this study was the optimization of a HPLC-MS method for the qualitative and quantitative analysis of asparagus saponins. The method includes extraction with aqueous ethanol, cleanup by solid phase extraction, separation by reverse phase chromatography, electrospray ionization, and detection in a single quadrupole mass analyzer. The method was used for the comparison of selected genotypes of Huétor-Tájar asparagus landrace and selected varieties of commercial diploid hybrids of green asparagus. The results showed that while protodioscin was almost the only saponin detected in the commercial hybrids, eight different saponins were detected in the Huétor-Tájar asparagus genotypes. The mass spectra indicated that HT saponins are derived from a furostan type steroidal genin having a single bond between carbons 5 and 6 of the B ring. The total concentration of saponins was found to be higher in triguero asparagus than in commercial hybrids.

  18. Pollen transmission of asparagus virus 2 (AV-2) may facilitate mixed infection by two AV-2 isolates in asparagus plants.

    Science.gov (United States)

    Kawamura, Ryusuke; Shimura, Hanako; Mochizuki, Tomofumi; Ohki, Satoshi T; Masuta, Chikara

    2014-09-01

    Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus and thought to induce the asparagus decline syndrome. AV-2 is known to be transmitted by seed, and the possibility of pollen transmission was proposed 25 years ago but not verified. In AV-2 sequence analyses, we have unexpectedly found mixed infection by two distinct AV-2 isolates in two asparagus plants. Because mixed infections by two related viruses are normally prevented by cross protection, we suspected that pollen transmission of AV-2 is involved in mixed infection. Immunohistochemical analyses and in situ hybridization using AV-2-infected tobacco plants revealed that AV-2 was localized in the meristem and associated with pollen grains. To experimentally produce a mixed infection via pollen transmission, two Nicotiana benthamiana plants that were infected with each of two AV-2 isolates were crossed. Derived cleaved-amplified polymorphic sequence analysis identified each AV-2 isolate in the progeny seedlings, suggesting that pollen transmission could indeed result in a mixed infection, at least in N. benthamiana.

  19. The parasitoids of the asparagus miner (Diptera: Agromyzidae): field parasitism and the influence of food resources on life history.

    Science.gov (United States)

    Morrison, William R; Gibson, Gary A P; Szendrei, Zsofia

    2014-12-01

    The goals of this study were to identify pupal parasitoids of the asparagus miner, Ophiomyia simplex Loew (Diptera: Agromyzidae), and examine the effect of different diets and floral resources on the lifespan of adult asparagus miners and their parasitoids. We also measured the effect of parasitism on stem damage caused by the asparagus miner. The identity and abundance of the parasitoids of the asparagus miner were determined in asparagus fields in Michigan from weekly asparagus miner pupal collections during the 2010-2013 seasons. Twelve species of hymenopterous parasitoids were reared from asparagus miner pupae, including Chorebus rondanii (Giard) (Ichneumonoidea: Braconidae), 10 species in three families of Chalcidoidea, and one species of Bethylidae (Chrysidoidea), that represent new host records for the asparagus miner. C. rondanii and Thinodytes cephalon (Walker) (Pteromalidae) were the most common parasitoids. The effects of different diets and flowers on the lifespan of the pest and parasitoid adults were also evaluated. Buckwheat resulted in the shortest life span for the asparagus miner, whereas Riddell's goldenrod significantly increased its lifespan relative to the control. Parasitoid lifespan was doubled when individuals were fed sugar-rich diets. In the field, parasitoids preferred stems that contained more pupae and damage. The two most commonly reared parasitoids should be considered as targets for future conservation biological control efforts of the asparagus miner.

  20. The parasitoids of the asparagus miner (Diptera: Agromyzidae): field parasitism and the influence of food resources on life history.

    Science.gov (United States)

    Morrison, William R; Gibson, Gary A P; Szendrei, Zsofia

    2014-12-01

    The goals of this study were to identify pupal parasitoids of the asparagus miner, Ophiomyia simplex Loew (Diptera: Agromyzidae), and examine the effect of different diets and floral resources on the lifespan of adult asparagus miners and their parasitoids. We also measured the effect of parasitism on stem damage caused by the asparagus miner. The identity and abundance of the parasitoids of the asparagus miner were determined in asparagus fields in Michigan from weekly asparagus miner pupal collections during the 2010-2013 seasons. Twelve species of hymenopterous parasitoids were reared from asparagus miner pupae, including Chorebus rondanii (Giard) (Ichneumonoidea: Braconidae), 10 species in three families of Chalcidoidea, and one species of Bethylidae (Chrysidoidea), that represent new host records for the asparagus miner. C. rondanii and Thinodytes cephalon (Walker) (Pteromalidae) were the most common parasitoids. The effects of different diets and flowers on the lifespan of the pest and parasitoid adults were also evaluated. Buckwheat resulted in the shortest life span for the asparagus miner, whereas Riddell's goldenrod significantly increased its lifespan relative to the control. Parasitoid lifespan was doubled when individuals were fed sugar-rich diets. In the field, parasitoids preferred stems that contained more pupae and damage. The two most commonly reared parasitoids should be considered as targets for future conservation biological control efforts of the asparagus miner. PMID:25313948

  1. Analysis on Pollution Factors in Asparagus Production and Research on Safety Production Technology

    Institute of Scientific and Technical Information of China (English)

    Liping; MA; Bianqing; HAO; Xiongwu; QIAO

    2013-01-01

    Based on the analysis on the infection degree,infection law and influencing factors of the main diseases on asparagus and the analysis on the pollution factors in asparagus production such as blind pesticide use,atmospheric pollution and acid rain,the pollution of soil and fertilizer,this article proposes asparagus safety production technologies which include the selection of disease-resistant variety and suitable planting field,scientific and reasonable disease control,balanced fertilization,rational irrigation,making a good job of field management, etc.,to reduce pathogenic factors.

  2. Asparagus polysaccharide and gum with hepatic artery embolization induces tumor growth and inhibits angiogenesis in an orthotopic hepatocellular carcinoma model.

    Science.gov (United States)

    Weng, Ling-Ling; Xiang, Jian-Feng; Lin, Jin-Bo; Yi, Shang-Hui; Yang, Li-Tao; Li, Yi-Sheng; Zeng, Hao-Tao; Lin, Sheng-Ming; Xin, Dong-Wei; Zhao, Hai-Liang; Qiu, Shu-Qi; Chen, Tao; Zhang, Min-Guang

    2014-01-01

    Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy.

  3. Asparagus polysaccharide and gum with hepatic artery embolization induces tumor growth and inhibits angiogenesis in an orthotopic hepatocellular carcinoma model.

    Science.gov (United States)

    Weng, Ling-Ling; Xiang, Jian-Feng; Lin, Jin-Bo; Yi, Shang-Hui; Yang, Li-Tao; Li, Yi-Sheng; Zeng, Hao-Tao; Lin, Sheng-Ming; Xin, Dong-Wei; Zhao, Hai-Liang; Qiu, Shu-Qi; Chen, Tao; Zhang, Min-Guang

    2014-01-01

    Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy. PMID:25605207

  4. Effect of chitosan and its derivatives as antifungal and preservative agents on postharvest green asparagus.

    Science.gov (United States)

    Qiu, Miao; Wu, Chu; Ren, Gerui; Liang, Xinle; Wang, Xiangyang; Huang, Jianying

    2014-07-15

    The antifungal activity and effect of high-molecular weight chitosan (H-chitosan), low-molecular weight chitosan (L-chitosan) and carboxymethyl chitosan (C-chitosan) coatings on postharvest green asparagus were evaluated. L-chitosan and H-chitosan efficiently inhibited the radial growth of Fusarium concentricum separated from postharvest green asparagus at 4 mg/ml, which appeared to be more effective in inhibiting spore germination and germ tube elongation than that of C-chitosan. Notably, spore germination was totally inhibited by L-chitosan and H-chitosan at 0.05 mg/ml. Coated asparagus did not show any apparent sign of phytotoxicity and maintained good quality over 28 days of cold storage, according to the weight loss and general quality aspects. Present results inferred that chitosan could act as an attractive preservative agent for postharvest green asparagus owing to its antifungal activity and its ability to stimulate some defense responses during storage.

  5. IN VIVO ANTI INFLAMMATORY AND ANTI ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF ASPARAGUS RACEMOSUS ROOTS

    Directory of Open Access Journals (Sweden)

    Suchita Mittal

    2013-04-01

    Full Text Available The present study is aimed to appraise the anti-inflammatory and anti-arthritic activity of ethanolic extract of Asparagus racemosus roots belonging to family Liliaceae. Carrageenan is used to induce inflammation and Freund’s Complete Adjuvant is used to induce arthritis. The result of this study revealed that Asparagus racemosus show potent effect on both the condition at a dose of 200mg/kg and 400mg/kg respectively.

  6. Short communication. Employment of molecular markers to develop tetraploid “supermale” asparagus from andromonoecious plants of the landrace ‘Morado de Huétor’

    Directory of Open Access Journals (Sweden)

    Jose J. Regalado

    2014-10-01

    Full Text Available The aim of this work was the development of new “supermale” genotypes retaining the highest genetic diversity possible of the tetraploid asparagus landrace ‘Morado de Huétor’. The elite andromonoecious male HT664 of the ‘Morado de Huétor’ asparagus landrace and the andromonoecious hybrid male HC027, obtained by crossing between this landrace and a commercial cultivar of Asparagus officinalis, were selected for self-pollination to produce possible “supermales” with genes of ‘Morado de Huétor’ (SMHT. To confirm the hybrid nature of HC027, we characterized this genotype with EST-SSR (Expressed Sequence Tag-derived Simple Sequence Repeats markers. We also adopted the sex-linked marker Asp1-T7 for sex determination in ‘Morado de Huétor’ and the resulting hybrids between this landrace and other commercial cultivars. Asp1-T7 marker was used for the selection and genotyping of SMHT. “All-male” cultivars with a different genetic background can be generated by crossing females with these new “supermale” genotypes, and the agronomical traits of these new cultivars would be very different from the “all-male” cultivars currently available in the market, making them extremely interesting for asparagus breeding programs.

  7. Improvement of Blood Pressure, Glucose Metabolism, and Lipid Profile by the Intake of Powdered Asparagus ( Lú Sŭn) Bottom-stems and Cladophylls.

    Science.gov (United States)

    Nishimura, Mie; Ohkawara, Tatsuya; Kagami-Katsuyama, Hiroyo; Sato, Hiroji; Nishihira, Jun

    2013-10-01

    Asparagus ( Lú Sǔn; Asparagus officinalis L.) is a common vegetable, long used as an herbal medicine. The cladophylls and bottom-stems of asparagus have various pharmacological effects, but they are generally discarded at harvesting. The present open clinical trial was performed to examine the effects of the intake of cladophylls and bottom-stems on the improvement of metabolic syndrome characterized by hypertension, hyperglycemia, and dyslipidemia. Twenty-eight healthy volunteers ingested either cladophyll or bottom-stem powder (6 g/day) daily for 10 weeks. The cladophyll intake resulted in significant reduction in the subjects' diastolic blood pressure and fasting plasma glucose (FPG), and decreased both the left cardio-ankle vascular index score and the total cholesterol level (T-CHO). The bottom-stem intake significantly reduced the subjects' systolic and diastolic blood pressure and FPG as well as T-CHO. These results suggest the possibility that asparagus cladophylls and bottom-stems differentially improve hypertension, hyperglycemia, and dyslipidemia. PMID:24716185

  8. Use of near-infrared reflectance spectroscopy for shelf-life discrimination of green asparagus stored in a cool room under controlled atmosphere.

    Science.gov (United States)

    Sánchez, María-Teresa; Pérez-Marín, Dolores; Flores-Rojas, Katherine; Guerrero, José-Emilio; Garrido-Varo, Ana

    2009-04-30

    This study sought to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to classify intact green asparagus, in refrigerated storage under controlled atmosphere, by storage time and post-harvest treatments applied. A total of 468 green asparagus (Asparagus officinalis, L., cultivar UC-157) were sampled after 7, 14, 21 and 28 days of refrigerated storage (2 degrees C, 95% R.H.) under three controlled atmosphere (CA) treatments: air (21 kPa O(2)+0.3 kPa CO(2)), CA(1) (5 kPa O(2)+5 kPa CO(2)) and CA(2) (10 kPa O(2)+10kPa CO(2)). Two commercially available spectrophotometers were evaluated for this purpose: a scanning monochromator (SM) of 400-2500 nm and a combination of diode array and scanning monochromator (DASM) of 350-2500 nm. Models developed using partial least squares 2-discriminant analysis (PLS2-DA) correctly classified between 81-100% of samples by post-harvest storage time, depending on the instrument used. Using similar models, the DASM instrument correctly classified 85% of samples by post-harvest treatment, compared with 72% using the SM. These results confirmed that NIR spectroscopy, coupled with the use of chemometric techniques, provides a reliable, accurate method of predicting the shelf-life of asparagus under different storage conditions and as a function of post-harvest treatment applied; the method can be readily applied at industrial level. PMID:19203619

  9. The potential of Thelypteris palustris and Asparagus sprengeri in phytoremediation of arsenic contamination.

    Science.gov (United States)

    Anderson, LaShunda L; Walsh, Maud; Roy, Amitava; Bianchetti, Christopher M; Merchan, Gregory

    2011-02-01

    The potential of two plants, Thelypteris palustris (marsh fern) and Asparagus sprengeri (asparagus fern), for phytoremediation of arsenic contamination was evaluated. The plants were chosen for this study because of the discovery of the arsenic hyperaccumulating fern, Pteris vittata (Ma et al., 2001) and previous research indicating asparagus fern's ability to tolerate > 1200 ppm soil arsenic. Objectives were (1) to assess if selected plants are arsenic hyperaccumulators; and (2) to assess changes in the species of arsenic upon accumulation in selected plants. Greenhouse hydroponic experiments arsenic treatment levels were established by adding potassium arsenate to solution. All plants were placed into the hydroponic experiments while still potted in their growth media. Marsh fern and Asparagus fern can both accumulate arsenic. Marsh fern bioaccumulation factors (> 10) are in the range of known hyperaccumulator, Pteris vittata Therefore, Thelypteris palustris is may be a good candidate for remediation of arsenic soil contamination levels of asparagus fern. The asparagus fern is arsenic tolerant (bioaccumulation factors < 10), but is not considered a good potential phytoremediation candidate.

  10. Keeping soil in the field - runoff and erosion management in asparagus crops

    Science.gov (United States)

    Niziolomski, Joanna; Simmons, Robert; Rickson, Jane; Hann, Mike

    2016-04-01

    Row crop production (including potatoes, onions, carrots, asparagus, bulbs and lettuce) is regarded as one of the most erosive agricultural cropping systems. This is a result of the many practices involved that increase erosion risk including: fine seedbed preparation, a typically short growing season where adequate ground cover protects the soil, permanent bare soil areas between crops, and often intensive harvesting methods that can damage soil structure and result in soil compaction. Sustained exposure of bare soil coupled with onsite compaction on slightly sloping land results in soil and water issues in asparagus production. Asparagus production is a growing British industry covering > 2000 ha and is worth approximately £30 million yr-1. However, no tried and tested erosion control measurements currently exist to manage associated problems. Research has recently been undertaken investigating the effectiveness of erosion control measures suitable for asparagus production systems. These consisted of surface applied wheat straw mulch and shallow soil disturbance (asparagus farm in Herefordshire, UK. Testing was conducted between May and November 2013. Rainfall-event based runoff and erosion measurements were taken including; runoff volume, runoff rate and total soil loss. Runoff and soil erosion was observed from all treatments. However, the surface application of straw mulch alone out performed each shallow soil disturbance practice. This suggests that runoff and erosion from asparagus production can be reduced using the simple surface application of straw.

  11. Anticancer effects of deproteinized asparagus polysaccharide on hepatocellular carcinoma in vitro and in vivo.

    Science.gov (United States)

    Xiang, Jianfeng; Xiang, Yanjie; Lin, Shengming; Xin, Dongwei; Liu, Xiaoyu; Weng, Lingling; Chen, Tao; Zhang, Minguang

    2014-04-01

    Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in the world whose chemoprevention became increasingly important in HCC treatment. Although the anticancer effects of asparagus constituents have been investigated in several cancers, its effects on hepatocellular carcinoma have not been fully studied. In this study, we investigated the anticancer effects of the deproteinized asparagus polysaccharide on the hepatocellular carcinoma cells using the in vitro and in vivo experimental model. Our data showed that deproteinized asparagus polysaccharide might act as an effective inhibitor on cell growth in vitro and in vivo and exert potent selective cytotoxicity against human hepatocellular carcinoma Hep3B and HepG2 cells. Further study showed that it could potently induce cell apoptosis and G2/M cell cycle arrest in the more sensitive Hep3B and HepG2 cell lines. Moreover, deproteinized asparagus polysaccharide potentiated the effects of mitomycin both in vitro and in vivo. Mechanistic studies revealed that deproteinized asparagus polysaccharide might exert its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. In conclusion, deproteinized asparagus polysaccharide exhibited significant anticancer activity against hepatocellular carcinoma cells and could sensitize the tumoricidal effects of mitomycin, indicating that it is a potential therapeutic agent (or chemosensitizer) for liver cancer therapy.

  12. Keeping soil in the field - runoff and erosion management in asparagus crops

    Science.gov (United States)

    Niziolomski, Joanna; Simmons, Robert; Rickson, Jane; Hann, Mike

    2016-04-01

    Row crop production (including potatoes, onions, carrots, asparagus, bulbs and lettuce) is regarded as one of the most erosive agricultural cropping systems. This is a result of the many practices involved that increase erosion risk including: fine seedbed preparation, a typically short growing season where adequate ground cover protects the soil, permanent bare soil areas between crops, and often intensive harvesting methods that can damage soil structure and result in soil compaction. Sustained exposure of bare soil coupled with onsite compaction on slightly sloping land results in soil and water issues in asparagus production. Asparagus production is a growing British industry covering > 2000 ha and is worth approximately £30 million yr‑1. However, no tried and tested erosion control measurements currently exist to manage associated problems. Research has recently been undertaken investigating the effectiveness of erosion control measures suitable for asparagus production systems. These consisted of surface applied wheat straw mulch and shallow soil disturbance (asparagus farm in Herefordshire, UK. Testing was conducted between May and November 2013. Rainfall-event based runoff and erosion measurements were taken including; runoff volume, runoff rate and total soil loss. Runoff and soil erosion was observed from all treatments. However, the surface application of straw mulch alone out performed each shallow soil disturbance practice. This suggests that runoff and erosion from asparagus production can be reduced using the simple surface application of straw.

  13. Asparagus racemosus: a review on its phytochemical and therapeutic potential.

    Science.gov (United States)

    Singh, Ram

    2016-09-01

    Asparagus racemosus (Willd.) is a widely found medicinal plant in tropical and subtropical parts of India. The therapeutic applications of this plant have been reported in Indian and British Pharmacopoeias and in traditional system of medicine, such as Ayurveda, Unani and Siddha. The crude, semi-purified and purified extracts obtained from different parts of this plant have been useful in therapeutic applications. Numerous bioactive phytochemicals mostly saponins and flavonoids have been isolated and identified from this plant which are responsible alone or in combination for various pharmacological activities. This review aims to give a comprehensive overview of traditional applications, current knowledge on the phytochemistry, pharmacology and overuse of A. racemosus. PMID:26463825

  14. Immunomodulatory potential of shatavarins produced from Asparagus racemosus tissue cultures.

    Science.gov (United States)

    Pise, Mashitha Vinod; Rudra, Jaishree Amal; Upadhyay, Avinash

    2015-01-01

    Medicinal properties of Asparagus racemosus (vernacular name: Shatavari) are attributed to its steroidal saponins called shatavarins. This plant is facing the threat of being endangered due to several developmental, seasonal constrains and malpractices involved in its collection and storage. To support its conservation, a tissue culture protocol is standardized which produces 20 fold higher levels of shatavarin. Here we evaluate the bioactivity and immunomodulatory potential of in vitro produced shatavarins from cell cultures of AR using human peripheral blood lymphocytes. In vitro produced shatavarin stimulated immune cell proliferation and IgG secretion in a dose dependent manner. It stimulated interleukin (IL)-12 production and inhibited production of IL-6. It also had strong modulatory effects on Th1/Th2 cytokine profile, indicating its potential application for immunotherapies where Th1/Th2 balance is envisaged. Our study demonstrating the bioactivity of tissue cultured AR extracts supports further in vivo evaluation of its immunomodulatory efficacy. PMID:26283842

  15. Effects of Genetic, Pre- and Post-Harvest Factors on Phenolic Content and Antioxidant Capacity of White Asparagus Spears

    Directory of Open Access Journals (Sweden)

    Evangelos Kazakis

    2009-12-01

    Full Text Available The effects of genetic, pre-harvest (season of harvest, spear diameter, spear portion and spear tip color and post-harvest factors (storage and domestic preparation practices, e.g., peeling and cooking on total phenolic, flavonoid and ascorbic acid content of white asparagus spears and their correlation with antioxidant capacity (DPPH and FRAP were studied. Results showed that genetic material was important for the total phenolic content but not season of harvest, spear diameter or storage. Violet spear tips and apical spear portions showed the largest amount of total phenolics. Peeling did not affect total phenolics in fresh asparagus, whereas it reduced their content in stored asparagus, while cooking resulted in an increase in both fresh and stored asparagus. However, the soluble extract of total phenolics and flavonoids were minor and the missing significance of phenolics and flavonoids in antioxidant capacity of white asparagus spears depends on these small amounts.

  16. Identification of flavonoid diglycosides in several genotypes of asparagus from the Huétor-Tájar population variety.

    Science.gov (United States)

    Fuentes-Alventosa, José María; Rodríguez, Guillermo; Cermeño, Pedro; Jiménez, Ana; Guillén, Rafael; Fernández-Bolaños, Juan; Rodríguez-Arcos, Rocío

    2007-11-28

    The qualitative and quantitative composition of flavonoids from the Huétor-Tájar population variety of asparagus (commonly known as " triguero") was investigated. Flavonoids were analyzed by reversed-phase high-performance liquid chromatography-diode array detection (HPLC-DAD). Liquid chromatography-mass spectrometry (LC-MS) under identical HPLC conditions was used to verify the identities of the flavonoid glycosides from triguero asparagus. The quantities of asparagus flavonoids were calculated according to concentration curves constructed with authentic standards. Total flavonoid contents, calculated as the sum of individual compounds, were determined and ranged from 400 to 700 mg/kg fresh weight. The most abundant was rutin, which represented 55-98% of the total flavonoid complement. Triguero asparagus were revealed to be an important source of not only quercetin derivatives but also kaempferol and isorhamnetin glycosides. Significant differences (p asparagus genotypes from the Huétor-Tájar population variety.

  17. Effects of genetic, pre- and post-harvest factors on phenolic content and antioxidant capacity of white asparagus spears.

    Science.gov (United States)

    Papoulias, Eleftherios; Siomos, Anastasios S; Koukounaras, Athanasios; Gerasopoulos, Dimitrios; Kazakis, Evangelos

    2009-12-16

    The effects of genetic, pre-harvest (season of harvest, spear diameter, spear portion and spear tip color) and post-harvest factors (storage and domestic preparation practices, e.g., peeling and cooking) on total phenolic, flavonoid and ascorbic acid content of white asparagus spears and their correlation with antioxidant capacity (DPPH and FRAP) were studied. Results showed that genetic material was important for the total phenolic content but not season of harvest, spear diameter or storage. Violet spear tips and apical spear portions showed the largest amount of total phenolics. Peeling did not affect total phenolics in fresh asparagus, whereas it reduced their content in stored asparagus, while cooking resulted in an increase in both fresh and stored asparagus. However, the soluble extract of total phenolics and flavonoids were minor and the missing significance of phenolics and flavonoids in antioxidant capacity of white asparagus spears depends on these small amounts.

  18. Effect of salicylic acid treatment on postharvest quality, antioxidant activities, and free polyamines of asparagus.

    Science.gov (United States)

    Wei, Yunxiao; Liu, Zhenfeng; Su, Yujing; Liu, Donghong; Ye, Xingqian

    2011-03-01

    The effects of salicylic acid (SA) on the quality and antioxidant activity of asparagus stored at 18 ± 2 °C were investigated by analyzing the color, chlorophyll, shear force, and the activity of antioxidant compounds such as ascorbic acid, phenolics, flavonoids, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity ferric reducing antioxidant power (FRAP), and polyamines (PAs). The results showed that SA improved the color and maintained the chlorophyll, phenolic, flavonoid, and ascorbic acid content of asparagus. High concentrations of SA caused a deterioration in asparagus would harm to color and had no effect on shear force within 6 d. SA induced the maximum concentration of phenolics in postharvest asparagus, promoted the increase in total flavonoids before 6 to 9 d, affected the antioxidant activity positively as indicated by the resultant increase in FRAP concentration; however, SA was only active with regard to DPPH scavenging activity within 6 d of treatment. Spermidine (Spd) is the most common form of PA in asparagus, and free putrescine (Put) contents increased over the first 3 d following harvest and then decreased. Spd and Spm concentrations evolved in a similar way and decreased during storage. Higher Spd and Spm contents in the SA pre-treatment Put was inhabited and its peaks appeared later.

  19. Evolution of Asparagus L. (Asparagaceae): Out-of-South-Africa and multiple origins of sexual dimorphism.

    Science.gov (United States)

    Norup, Maria F; Petersen, Gitte; Burrows, Sandie; Bouchenak-Khelladi, Yanis; Leebens-Mack, Jim; Pires, J Chris; Linder, H Peter; Seberg, Ole

    2015-11-01

    In the most comprehensive study to date we explored the phylogeny and evolution of the genus Asparagus, with emphasis on the southern African species. We included 211 accessions, representing 77 (92%) of the southern African, 6 (17%) of the tropical African, 10 (56%) of the strictly European and 6 (9%) of the Eurasian species. We analyzed DNA sequences from three plastid regions (trnH-psbA, trnD-T, ndhF) and from the nuclear region phytochrome C (PHYC) with parsimony and maximum likelihood methods, and recovered a monophyletic Asparagus. The phylogeny conflicts with all previous infra-generic classifications. It has many strongly supported clades, corroborated by morphological characters, which may provide a basis for a revised taxonomy. Additionally, the phylogeny indicates that many of the current species delimitations are problematic. Using biogeographic analyses that account for phylogenetic uncertainty (S-DIVA) and take into account relative branch lengths (Lagrange) we confirm the origin of Asparagus in southern Africa, and find no evidence that the dispersal of Asparagus follow the Rand flora pattern. We find that all truly dioecious species of Asparagus share a common origin, but that sexual dimorphism has arisen independently several times. PMID:26079131

  20. A study Antiurolithiatic Activity of ethanolic extract of Asparagus racemosus in animal models

    Directory of Open Access Journals (Sweden)

    Jagannath N

    2015-12-01

    Full Text Available Objective: To investigate the Antiurolithiatic Activity of ethanolic extract of Asparagus racemosus in animal models.Materials and Methods: The study includes performing on healthy albino rats of either sex weighing 220 – 270gms and urolithiasis was induced by oral administration of ethylene glycol and ammonium chloride water. The parameters studied are serum analysis for Urea, Creatinine, Calcium and Phosphorus, Body Weight of animals included in the study group and Histopathological Study of kidney for the presences crystals.  Results In our study the Ethanolic extract of Asparagus Racemosus with doses of 800mg/kg and 1600mg/kg per orally to rats showed significant reduction in serum urea, creatinine, calcium and phosphorus levels in urolithiatic rats when compared to the positive control rats (Group II. These results were found to be statistically significant (p<0.05.Conclusion: Ethanol Extract of Asparagus racemosus has a significant antiurolithiatic activity.

  1. Effect of 1-methylcyclopropene treatment on green asparagus quality during cold storage

    Science.gov (United States)

    Zhang, Peng; Zhang, Min; Wang, Shaojin; Wu, Zhishuang

    2012-10-01

    Green asparagus was treated with 1-methylcyclopropene at three concentration levels at room temperature for 24 h after harvest to evaluate the postharvest quality during cold storage at 4°C. Comparing with the controls, the loss of vitamin C, decomposition of chlorophyll, and accumulation of the malonydiadehyde under treatments of 1-methylcyclopropene were reduced during storage. The enzyme activities in asparagus including peroxidase and phenylalanine ammonia lyase were inhibited by 1-methylcyclopropene treatments, while the activity of superoxide dismutase was enhanced. Based on non-significant difference of the treated samples with 6 ìl l-1, 1-methylcyclopropene treatments at 4 ìl l-1 could be selected to maintain postharvest quality of green asparagus and provide long storage life.

  2. BROWN SPOT CAUSED BY CURVULARIA SPP., A NEW DISEASE OF ASPARAGUS

    Directory of Open Access Journals (Sweden)

    B. SALLEH

    1996-01-01

    Full Text Available The distribution, aetiology and symptomatology of a new disease on asparagus ferns, which we have termed brown spot, is described. Descriptions of and a key to identification of the causal organisms, Curvularia brachyspora, C. eragrostidis, C. lunata and C. pallescens, are also presented. Pathogenicity tests showed that C. lunata was the dominant and most virulent of the four species. Inoculation with conidial suspensions or mycelial transfers through wounded ferns were more effective in inducing the disease than inoculations on unwounded ferns. This is the first record of C. brachyspora in Malaysia and the first report of this disease on asparagus.

  3. Effect of methanolic extract of Asparagus racemosus Willd. on lipopolysaccharide induced-oxidative stress in rats.

    Science.gov (United States)

    Ahmad, Mohammad Parwez; Hussain, Arshad; Siddiqui, Hefazat Hussain; Wahab, Shadma; Adak, Manoranjan

    2015-03-01

    Lipopolysaccharide (LPS) induced oxidative stress and impairment of normal physiological function generally categorized by increased anxiety and reduced mobility. Therefore, the present study was to find out the effect Methanolic extract of Asparagus racemosus (MEAR ) in lipopolysaccharide (LPS)-induced oxidative stress in rats . LPS-induced oxidative stress in rats was measured by locomotor activity by photoactometer test, anxiety with elevated plus maze test and also studied the oxidative stress markers, nitric oxide and cytokines. The obtained data shows that LPS markedly exhausted (pAsparagus racemosus Willd. is a functionally newer type of cerebroprotective agent.

  4. Effect of methanolic extract of Asparagus racemosus Willd. on lipopolysaccharide induced-oxidative stress in rats.

    Science.gov (United States)

    Ahmad, Mohammad Parwez; Hussain, Arshad; Siddiqui, Hefazat Hussain; Wahab, Shadma; Adak, Manoranjan

    2015-03-01

    Lipopolysaccharide (LPS) induced oxidative stress and impairment of normal physiological function generally categorized by increased anxiety and reduced mobility. Therefore, the present study was to find out the effect Methanolic extract of Asparagus racemosus (MEAR ) in lipopolysaccharide (LPS)-induced oxidative stress in rats . LPS-induced oxidative stress in rats was measured by locomotor activity by photoactometer test, anxiety with elevated plus maze test and also studied the oxidative stress markers, nitric oxide and cytokines. The obtained data shows that LPS markedly exhausted (pAsparagus racemosus Willd. is a functionally newer type of cerebroprotective agent. PMID:25730806

  5. Genetic evaluation of twenty seed sources of Asparagus racemosus

    Institute of Scientific and Technical Information of China (English)

    Parveen; A Kumar; H.S.Ginwal

    2011-01-01

    A field trial of 20 seed sources of Asparagus racemosus was conducted at the Forest Research Institute, Dehradun, Uttarakhand, India to evaluate their performance of different economic traits. Genotypic variance, phenotypic variance, genotypic coefficient of variance (GCV)and phenotypic coefficient of variance (PCV) for number of shoots,shoot height, shoot weight, number of roots, root length, root diameter and root weight were calculated. Maximum genotypic and phenotypic variance was observed in shoot height among the shoot - related traits and root length among the root - related traits. For the shoot height, genotypic variance, phenotypic variance, genotypic coefficient of variance,phenotypic coefficient of variance were 231.80, 3924.80, 61.26 and 1037.32, respectively, where those of the root length were 9.55, 16.80,23.46 and 41.27, respectively. The maximum genetic advance and genetic gain were obtained for shoot height among the shoot-related traits and root length among the root-related traits. Index values were developed for all the seed sources based on the four most important traits, and Panthnagar (Uttrakhand), Jodhpur (Rajasthan), Dehradun (Uttarakhand),Chandigarh (Punjab), Jammu (Jammu and Kashmir) and Solan (Himachal Pradesh), were promising seed sources for root production.

  6. Free Radicals, Salicylic Acid and Mycotoxins in Asparagus After Inoculation with Fusarium proliferatum and F. oxysporum.

    Science.gov (United States)

    Dobosz, Bernadeta; Drzewiecka, Kinga; Waskiewicz, Agnieszka; Irzykowska, Lidia; Bocianowski, Jan; Karolewski, Zbigniew; Kostecki, Marian; Kruczynski, Zdzislaw; Krzyminiewski, Ryszard; Weber, Zbigniew; Golinski, Piotr

    2011-09-01

    Electron paramagnetic resonance was used to monitor free radicals and paramagnetic species like Fe, Mn, Cu generation, stability and status in Asparagus officinalis infected by common pathogens Fusarium proliferatum and F. oxysporum. Occurrence of F. proliferatum and F. oxysporum, level of free radicals and other paramagnetic species, as well as salicylic acid and mycotoxins content in roots and stems of seedlings were estimated on the second and fourth week after inoculation. In the first term free and total salicylic acid contents were related to free radicals level in stem (P = 0.010 and P = 0.033, respectively). Concentration of Fe(3+) ions in porphyrin complexes (g = 2.3, g = 2.9) was related to the species of pathogen. There was no significant difference between Mn(2+) concentrations in stem samples; however, the level of free radicals in samples inoculated with F. proliferatum was significantly higher when compared to F. oxysporum. PMID:21957331

  7. The effect of plant growth-promoting rhizobacteria on asparagus seedlings and germinating seeds subjected to water stress under greenhouse conditions.

    Science.gov (United States)

    Liddycoat, Scott M; Greenberg, Bruce M; Wolyn, David J

    2009-04-01

    Plant growth-promoting rhizobacteria (PGPR) can have positive effects on vigour and productivity, especially under stress conditions. In asparagus (Asparagus officinalis L.) field culture, seeds are planted in high-density nurseries, and 1-year-old crowns are transplanted to production fields. Performance can be negatively affected by water stress, transplant shock, and disease pressure on wounded roots. PGPR inoculation has the potential to alleviate some of the stresses incurred in the production system. In this study, the effects of PGPR (Pseudomonas spp.) treatment were determined on 3-week-old greenhouse-grown seedlings and germinating seeds of 2 asparagus cultivars. The pots were irrigated to a predetermined level that resulted in optimum growth or the plants were subjected to drought or flooding stress for 8 weeks. The cultivars responded differently to PGPR: single inoculations of seedlings enhanced growth of 'Guelph Millennium' under optimum conditions and 'Jersey Giant' seedlings under drought stress. Seed inoculations with PGPR resulted in a positive response only for 'Guelph Millennium', for which both single or multiple inoculations enhanced plant growth under drought stress.

  8. The effect of plant growth-promoting rhizobacteria on asparagus seedlings and germinating seeds subjected to water stress under greenhouse conditions.

    Science.gov (United States)

    Liddycoat, Scott M; Greenberg, Bruce M; Wolyn, David J

    2009-04-01

    Plant growth-promoting rhizobacteria (PGPR) can have positive effects on vigour and productivity, especially under stress conditions. In asparagus (Asparagus officinalis L.) field culture, seeds are planted in high-density nurseries, and 1-year-old crowns are transplanted to production fields. Performance can be negatively affected by water stress, transplant shock, and disease pressure on wounded roots. PGPR inoculation has the potential to alleviate some of the stresses incurred in the production system. In this study, the effects of PGPR (Pseudomonas spp.) treatment were determined on 3-week-old greenhouse-grown seedlings and germinating seeds of 2 asparagus cultivars. The pots were irrigated to a predetermined level that resulted in optimum growth or the plants were subjected to drought or flooding stress for 8 weeks. The cultivars responded differently to PGPR: single inoculations of seedlings enhanced growth of 'Guelph Millennium' under optimum conditions and 'Jersey Giant' seedlings under drought stress. Seed inoculations with PGPR resulted in a positive response only for 'Guelph Millennium', for which both single or multiple inoculations enhanced plant growth under drought stress. PMID:19396238

  9. Research Progresses of Asparagus in Cultivation Technology and Post-harvest Processing Technology in China%我国芦笋栽培及采后贮藏加工技术研究进展

    Institute of Scientific and Technical Information of China (English)

    谭亮萍; 贺超兴; 戴雄泽; 孙超; 马庆辉; 李振泉

    2011-01-01

    The trend of asparagus (Asparagus officinalis L.) planting all over the world is that developed countries reduce the areas, but the developing countries expand the areas gradually.Planting asparagus developed rapidly in China.China is the biggest country of asparagus production and export in the world.Recent research progress on its cultivation technology, post-harvest physiology, preservation,nutritional and medical function are reviewed.The existing problems in asparagus production are analyzed,and the future development prospect for asparagus industry is expected in this paper.%全世界芦笋种植的趋势是发达国家面积不断减少,发展中国家面积逐渐扩大,绿芦笋种植面积增加,白芦笋面积减少.我国在20世纪80~90年代芦笋种植发展较快,截至2008年全世界芦笋栽培面积约为25.3万hm2,其中亚洲达12万hm2,而我国约占10万hm2,我国已成为世界上栽培面积最大的芦笋生产国和产品出口国.本文综述了我国芦笋栽培技术、采后贮藏加工技术及营养功能成分等方面的研究进展,并对生产中存在的问题进行了分析,对芦笋产业的发展前景进行了展望.

  10. Host-plant finding by the asparagus fly, Plioreocepta poeciloptera (Diptera: Tephritidae), a monophagous, monovoltine tephritid.

    Science.gov (United States)

    Thibout, E; Pierre, D; Mondy, N; Lecomte, C; Biémont, J C; Auger, J

    2005-10-01

    The role of various olfactory and visual stimuli was studied in host-plant finding by the asparagus fly Plioreocepta poeciloptera (Schrank), a monophagous monovoltine tephritid causing serious damage to asparagus spears. Volatiles released by asparagus plants were extracted by diethyl ether after cryotrapping concentration, and identified by gas chromatography-mass spectrometry. Twelve of the 13 compounds identified were tested using electroantennography to measure the response of the fly. Behavioural response was analysed using two different flight tunnels according to circadian rhythm, age and sex of adults, presence of the plant and of different coloured lures, presence of a male congener, or exposure to four pure asparagus odour compounds that elicited responses in electroantennography, i.e. hexanal, (E)-2-hexenal, (Z)-2-hexen-1-ol and decanal. Data showed that males locate the host plant more quickly than females. Females are attracted mainly by the blend of plant odour and male pheromone. Both sexes respond to a complex of stimuli only during the afternoon. These findings will be helpful in developing new and effective approaches to control this pest insect.

  11. Evolution of quality characteristics of minimally processed asparagus during storage in different lighting conditions.

    Science.gov (United States)

    Sanz, S; Olarte, C; Ayala, F; Echávarri, J F

    2009-08-01

    The effect of different types of lighting (white, green, red, and blue light) on minimally processed asparagus during storage at 4 degrees C was studied. The gas concentrations in the packages, pH, mesophilic counts, and weight loss were also determined. Lighting caused an increase in physiological activity. Asparagus stored under lighting achieved atmospheres with higher CO(2) and lower O(2) content than samples kept in the dark. This activity increase explains the greater deterioration experienced by samples stored under lighting, which clearly affected texture and especially color, accelerating the appearance of greenish hues in the tips and reddish-brown hues in the spears. Exposure to light had a negative effect on the quality parameters of the asparagus and it caused a significant reduction in shelf life. Hence, the 11 d shelf life of samples kept in the dark was reduced to only 3 d in samples kept under red and green light, and to 7 d in those kept under white and blue light. However, quality indicators such as the color of the tips and texture showed significantly better behavior under blue light than with white light, which allows us to state that it is better to use this type of light or blue-tinted packaging film for the display of minimally processed asparagus to consumers.

  12. "Asparagus Racemosus" Enhances Memory and Protects against Amnesia in Rodent Models

    Science.gov (United States)

    Ojha, Rakesh; Sahu, Alakh N.; Muruganandam, A. V.; Singh, Gireesh Kumar; Krishnamurthy, Sairam

    2010-01-01

    "Asparagus Racemosus" (AR) is an Ayurvedic rasayana possessing multiple neuropharmacological activities. The adpatogenic and antidepressant activity of AR is well documented. The present study was undertaken to assess nootropic and anti-amnesic activities of MAR in rats. The Morris water maze (MWM) and elevated plus maze (EPM) models were employed…

  13. Automatic harvesting of asparagus: an application of robot vision to agriculture

    Science.gov (United States)

    Grattoni, Paolo; Cumani, Aldo; Guiducci, Antonio; Pettiti, Giuseppe

    1994-02-01

    This work presents a system for the automatic selective harvesting of asparagus in open field being developed in the framework of the Italian National Project on Robotics. It is composed of a mobile robot, equipped with a suitable manipulator, and driven by a stereo-vision module. In this paper we discuss in detail the problems related to the vision module.

  14. Changes in microbial diversity of brined green asparagus upon treatment with high hydrostatic pressure.

    Science.gov (United States)

    Toledo Del Árbol, Julia; Pérez Pulido, Rubén; La Storia, Antonietta; Grande Burgos, Maria José; Lucas, Rosario; Ercolini, Danilo; Gálvez, Antonio

    2016-01-01

    The application of high hydrostatic pressure (HHP, 600MPa, 8 min) on brined green asparagus and the changes in bacterial diversity after treatments and during storage at 4 °C (30 days) or 22 °C (10 days) were studied. HHP treatments reduced viable cell counts by 3.6 log cycles. The residual surviving population did not increase during storage at 4 °C. However, bacterial counts significantly increased at 22 °C by day 3, leading to rapid spoilage. The microbiota of green asparagus was composed mainly by Proteobacteria (mainly Pantoea and Pseudomonas), followed by Firmicutes (mainly Lactococcus and Enterococcus) and to a less extent Bacteroidetes and Actinobacteria. During chill storage of untreated asparagus, the relative abundance of Proteobacteria as well as Enterococcus and Lactococcus decreased while Lactobacillus increased. During storage of untreated asparagus at 22 °C, the abundance of Bacteroidetes decreased while Proteobacteria increased during late storage. The HHP treatment determined a reduction of the Proteobacteria both early after treatment and during chill storage. In the HHP treated samples stored at 22 °C, the relative abundance of Pseudomonas rapidly decreased at day 1, with an increase of Bacteroidetes. This was followed by a marked increase in Enterobacteriaceae (Escherichia) simultaneously with increase in viable counts and spoilage. Results from the study indicate that the effect of HHP treatments on the viability ofmicrobial populations in foods also has an impact on the dynamics of microbial populations during the storage of the treated foods.

  15. Relationships between Fusarium population structure, soil nutrient status and disease incidence in field-grown asparagus

    NARCIS (Netherlands)

    Yergeau, E.; Sommerville, D.W.; Maheux, E.; Vujanovic, V.; Hamel, C.; Whalen, J.K.; St-Arnaud, M.

    2006-01-01

    Fusarium species cause important diseases in many crops. Lack of knowledge on how Fusarium species and strains interact with their environment hampers growth management strategies to control root diseases. A field experiment involving asparagus as host plant and three phosphorus fertilization levels

  16. Changes in microbial diversity of brined green asparagus upon treatment with high hydrostatic pressure.

    Science.gov (United States)

    Toledo Del Árbol, Julia; Pérez Pulido, Rubén; La Storia, Antonietta; Grande Burgos, Maria José; Lucas, Rosario; Ercolini, Danilo; Gálvez, Antonio

    2016-01-01

    The application of high hydrostatic pressure (HHP, 600MPa, 8 min) on brined green asparagus and the changes in bacterial diversity after treatments and during storage at 4 °C (30 days) or 22 °C (10 days) were studied. HHP treatments reduced viable cell counts by 3.6 log cycles. The residual surviving population did not increase during storage at 4 °C. However, bacterial counts significantly increased at 22 °C by day 3, leading to rapid spoilage. The microbiota of green asparagus was composed mainly by Proteobacteria (mainly Pantoea and Pseudomonas), followed by Firmicutes (mainly Lactococcus and Enterococcus) and to a less extent Bacteroidetes and Actinobacteria. During chill storage of untreated asparagus, the relative abundance of Proteobacteria as well as Enterococcus and Lactococcus decreased while Lactobacillus increased. During storage of untreated asparagus at 22 °C, the abundance of Bacteroidetes decreased while Proteobacteria increased during late storage. The HHP treatment determined a reduction of the Proteobacteria both early after treatment and during chill storage. In the HHP treated samples stored at 22 °C, the relative abundance of Pseudomonas rapidly decreased at day 1, with an increase of Bacteroidetes. This was followed by a marked increase in Enterobacteriaceae (Escherichia) simultaneously with increase in viable counts and spoilage. Results from the study indicate that the effect of HHP treatments on the viability ofmicrobial populations in foods also has an impact on the dynamics of microbial populations during the storage of the treated foods. PMID:26372734

  17. Study on the Technical Parameters of Asparagus Juice Extraction and Asparagus Wine Fermentation%芦笋汁浸提及芦笋酒发酵工艺参数研究

    Institute of Scientific and Technical Information of China (English)

    侍朋宝; 陈海菊; 常学东; 王玉军

    2011-01-01

    [目的]得出芦笋汁浸提及芦笋酒发酵工艺参数.[方法]采用正交试验设计对芦笋汁浸提影响因素进行研究,并采用3种不同工艺对芦笋酒进行了酿造.[结果]芦笋汁浸提的最佳工艺参数为浸提温度35℃,pH 4.0,浸提时间8h,酶用量0.03 g/L,该浸提条件下,芦笋汁的提取率为58.12%.该试验条件下浸提后的芦笋浆对芦笋渣进行全程浸渍发酵所得芦笋酒质量最好,皂土澄清该芦笋酒的最佳用量为600 mg/L.[结论]研究为芦笋下脚料在酿酒方面的开发利用提供了参考.%[Objective] The aim was to obtain the technical parameters of asparagus juice extraction and asparagus wine fermenting. [ Method] The orthogonal experimental design was used to study the influencing factors of asparagus juice, and three different processes for making asparagus wine were studied. [ Result ] The optimal extraction conditions of asparagus juice were as follows: the extraction temperature was 35℃ , pH was 4.0, extraction time was 8 h and the enzyme dosage was 0.03 g/L. Under this condition, the yield of asparagus juice was up to 58.12%. High quality asparagus wine can be made by the process of macerating asparagus from start to end during fermenting, suitable bentonite dosage for clarificating asparagus wine was 600 mg/L. [ Conclusion] The research provides reference for development and utilization of leftover parts of asparagus in aspect of making asparagus wine.

  18. Rose Prickles and Asparagus Spines--Different Hook Structures as Attachment Devices in Climbing Plants.

    Directory of Open Access Journals (Sweden)

    Friederike Gallenmüller

    Full Text Available Functional morphology and biomechanical properties of hook structures functioning as attachment devices in the leaning climbers Rosa arvensis, Rosa arvensis 'Splendens', Asparagus falcatus and Asparagus setaceus are analysed in order to investigate the variability in closely related species as well as convergent developments of hook structure and properties in distant systematic lineages (monocots and dicots. Prickles and spines were characterised by their size, orientation and the maximum force measured at failure in mechanical tests performed with traction forces applied at different angles. In Rosa arvensis and Rosa arvensis 'Splendens' three types of prickles differing largely in geometrical and mechanical properties are identified (prickles of the wild species and two types of prickles in the cultivar. In prickles of Rosa arvensis no particular orientation of the prickle tip is found whereas in the cultivar Rosa arvensis 'Splendens' prickles gradually gain a downward-orientation due to differential growth in the first weeks of their development. Differences in mechanical properties and modes of failure are correlated to geometrical parameters. In Asparagus falcatus and Asparagus setaceus spines are composed of leaf tissue, stem tissue and tissue of the axillary bud. Between species spines differ in size, orientation, distribution along the stem, tissue contributions and mechanical properties. The prickles of Rosa arvensis and its cultivar and the spines of the studied Asparagus species have several traits in common: (1 a gradual change of cell size and cell wall thickness, with larger cells in the centre and smaller thick-walled cells at the periphery of the hooks, (2 occurrence of a diversity of shape and geometry within one individual, (3 failure of single hooks when submitted to moderate mechanical stresses (Fmax/basal area < 35 N/mm² and (4 failure of the hooks without severe stem damage (at least in the tested wild species.

  19. Rose Prickles and Asparagus Spines--Different Hook Structures as Attachment Devices in Climbing Plants.

    Science.gov (United States)

    Gallenmüller, Friederike; Feus, Amélie; Fiedler, Kathrin; Speck, Thomas

    2015-01-01

    Functional morphology and biomechanical properties of hook structures functioning as attachment devices in the leaning climbers Rosa arvensis, Rosa arvensis 'Splendens', Asparagus falcatus and Asparagus setaceus are analysed in order to investigate the variability in closely related species as well as convergent developments of hook structure and properties in distant systematic lineages (monocots and dicots). Prickles and spines were characterised by their size, orientation and the maximum force measured at failure in mechanical tests performed with traction forces applied at different angles. In Rosa arvensis and Rosa arvensis 'Splendens' three types of prickles differing largely in geometrical and mechanical properties are identified (prickles of the wild species and two types of prickles in the cultivar). In prickles of Rosa arvensis no particular orientation of the prickle tip is found whereas in the cultivar Rosa arvensis 'Splendens' prickles gradually gain a downward-orientation due to differential growth in the first weeks of their development. Differences in mechanical properties and modes of failure are correlated to geometrical parameters. In Asparagus falcatus and Asparagus setaceus spines are composed of leaf tissue, stem tissue and tissue of the axillary bud. Between species spines differ in size, orientation, distribution along the stem, tissue contributions and mechanical properties. The prickles of Rosa arvensis and its cultivar and the spines of the studied Asparagus species have several traits in common: (1) a gradual change of cell size and cell wall thickness, with larger cells in the centre and smaller thick-walled cells at the periphery of the hooks, (2) occurrence of a diversity of shape and geometry within one individual, (3) failure of single hooks when submitted to moderate mechanical stresses (Fmax/basal area < 35 N/mm²) and (4) failure of the hooks without severe stem damage (at least in the tested wild species).

  20. Rose Prickles and Asparagus Spines--Different Hook Structures as Attachment Devices in Climbing Plants.

    Science.gov (United States)

    Gallenmüller, Friederike; Feus, Amélie; Fiedler, Kathrin; Speck, Thomas

    2015-01-01

    Functional morphology and biomechanical properties of hook structures functioning as attachment devices in the leaning climbers Rosa arvensis, Rosa arvensis 'Splendens', Asparagus falcatus and Asparagus setaceus are analysed in order to investigate the variability in closely related species as well as convergent developments of hook structure and properties in distant systematic lineages (monocots and dicots). Prickles and spines were characterised by their size, orientation and the maximum force measured at failure in mechanical tests performed with traction forces applied at different angles. In Rosa arvensis and Rosa arvensis 'Splendens' three types of prickles differing largely in geometrical and mechanical properties are identified (prickles of the wild species and two types of prickles in the cultivar). In prickles of Rosa arvensis no particular orientation of the prickle tip is found whereas in the cultivar Rosa arvensis 'Splendens' prickles gradually gain a downward-orientation due to differential growth in the first weeks of their development. Differences in mechanical properties and modes of failure are correlated to geometrical parameters. In Asparagus falcatus and Asparagus setaceus spines are composed of leaf tissue, stem tissue and tissue of the axillary bud. Between species spines differ in size, orientation, distribution along the stem, tissue contributions and mechanical properties. The prickles of Rosa arvensis and its cultivar and the spines of the studied Asparagus species have several traits in common: (1) a gradual change of cell size and cell wall thickness, with larger cells in the centre and smaller thick-walled cells at the periphery of the hooks, (2) occurrence of a diversity of shape and geometry within one individual, (3) failure of single hooks when submitted to moderate mechanical stresses (Fmax/basal area < 35 N/mm²) and (4) failure of the hooks without severe stem damage (at least in the tested wild species). PMID:26629690

  1. QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits

    OpenAIRE

    Xu, Pei; Wu, Xiaohua; Wang, Baogen; Hu, Tingting; Lu, Zhongfu; Liu, Yonghua; Qin, Dehui; Wang, Sha; Li, Guojing

    2013-01-01

    Background Asparagus bean (Vigna. unguiculata. ssp sesquipedalis) is a subspecies and special vegetable type of cowpea (Vigna. unguiculata L. Walp.) important in Asia. Genetic basis of horticulturally important traits of asparagus bean is still poorly understood, hindering the utilization of targeted, DNA marker-assisted breeding in this crop. Here we report the identification of quantitative trait loci (QTLs) and epistatic interactions for four horticultural traits, namely, days to first flo...

  2. A SNP and SSR based genetic map of asparagus bean (Vigna. unguiculata ssp. sesquipedialis and comparison with the broader species.

    Directory of Open Access Journals (Sweden)

    Pei Xu

    Full Text Available Asparagus bean (Vigna. unguiculata ssp. sesquipedialis is a distinctive subspecies of cowpea [Vigna. unguiculata (L. Walp.] that apparently originated in East Asia and is characterized by extremely long and thin pods and an aggressive climbing growth habit. The crop is widely cultivated throughout Asia for the production of immature pods known as 'long beans' or 'asparagus beans'. While the genome of cowpea ssp. unguiculata has been characterized recently by high-density genetic mapping and partial sequencing, little is known about the genome of asparagus bean. We report here the first genetic map of asparagus bean based on SNP and SSR markers. The current map consists of 375 loci mapped onto 11 linkage groups (LGs, with 191 loci detected by SNP markers and 184 loci by SSR markers. The overall map length is 745 cM, with an average marker distance of 1.98 cM. There are four high marker-density blocks distributed on three LGs and three regions of segregation distortion (SDRs identified on two other LGs, two of which co-locate in chromosomal regions syntenic to SDRs in soybean. Synteny between asparagus bean and the model legume Lotus. japonica was also established. This work provides the basis for mapping and functional analysis of genes/QTLs of particular interest in asparagus bean, as well as for comparative genomics study of cowpea at the subspecies level.

  3. Automatic Recognition Of Moving Objects And Its Application To A Robot For Picking Asparagus

    Science.gov (United States)

    Baylou, P.; Amor, B. El Hadj; Bousseau, G.

    1983-10-01

    After a brief description of the robot for picking white asparagus, a statistical study of the different shapes of asparagus tips allowed us to determine certain discriminating parameters to detect the tips as they appear on the silhouette of the mound of earth. The localisation was done stereometrically with the help of two cameras. As the robot carrying the system of vision-localisation moves, the images are altered and decision cri-teria modified. A study of the image from mobile objects produced by both tube and CCD came-ras was carried out. A simulation of this phenomenon has been achieved in order to determine the modifications concerning object shapes, thresholding levels and decision parameters in function of the robot speed.

  4. Characterisation of kiwifruit and asparagus enzyme extracts, and their activities toward meat proteins.

    Science.gov (United States)

    Ha, Minh; Bekhit, Alaa El-Din; Carne, Alan; Hopkins, David L

    2013-01-15

    Two plant enzyme extracts from kiwifruit and asparagus were evaluated for their ability to hydrolyse commercially available substrates and proteins present in both beef connective tissue and topside myofibrillar extracts. The results show significant differences in protease activity depending on the assay used. Protease assays with connective tissue and meat myofibrillar extracts provide a more realistic evaluation of the potential of the enzymes for application in meat tenderization. Overall, the kiwifruit protease extract was found to be more effective at hydrolysing myofibrillar and collagen proteins than the asparagus protease extract. The two protease extracts appeared to target meat myofibrillar and collagen proteins differently, suggesting the potential of a synergistic effect of these proteases in improving the tenderness of specific cuts of meat, based on their intrinsic protein composition.

  5. Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods.

    Science.gov (United States)

    Wang, Mingfu; Tadmor, Yaakov; Wu, Qing-Li; Chin, Chee-Kok; Garrison, Stephen A; Simon, James E

    2003-10-01

    A liquid chromatography/mass spectrometry (LC/MS) method with selected ion monitoring was developed and validated to analyze the contents of protodioscin and rutin in asparagus. The distribution of rutin and protodioscin within the shoots was found to vary by location, with the tissue closest to the rhizome found to be a rich source of protodioscin, at an average level of 0.025% tissue fresh weight in the three tested lines, while the upper youngest shoot tissue contained the highest amount of rutin at levels of 0.03-0.06% tissue fresh weight. The lower portions of the asparagus shoots that are discarded during grading and processing should instead be considered a promising source of a new value-added nutraceutical product.

  6. Characterisation of kiwifruit and asparagus enzyme extracts, and their activities toward meat proteins.

    Science.gov (United States)

    Ha, Minh; Bekhit, Alaa El-Din; Carne, Alan; Hopkins, David L

    2013-01-15

    Two plant enzyme extracts from kiwifruit and asparagus were evaluated for their ability to hydrolyse commercially available substrates and proteins present in both beef connective tissue and topside myofibrillar extracts. The results show significant differences in protease activity depending on the assay used. Protease assays with connective tissue and meat myofibrillar extracts provide a more realistic evaluation of the potential of the enzymes for application in meat tenderization. Overall, the kiwifruit protease extract was found to be more effective at hydrolysing myofibrillar and collagen proteins than the asparagus protease extract. The two protease extracts appeared to target meat myofibrillar and collagen proteins differently, suggesting the potential of a synergistic effect of these proteases in improving the tenderness of specific cuts of meat, based on their intrinsic protein composition. PMID:23122154

  7. 芦笋保鲜技术研究进展%Advances in Preservation Technology of Asparagus

    Institute of Scientific and Technical Information of China (English)

    瞿华香; 张岳平; 宋晓; 郑立平; 陈光宇

    2013-01-01

    Asparagus is an important high nutrition vegetable and rich in a variety of bioactive substance,which has good nutritional and medicinal properties.In recent years,the development of asparagus industry was rapid in China.However,as a fresh edible vegetable,preservation technology of asparagus is a growing key point due to its high breathing and strong metabolic characteristics after harvest.In this paper,the current status of asparagus preservation technology system and mechanism of affecting asparagus quality after harvest were reviewed,moreover,the developing trend of asparagus preservation technology was prospected.These results can provide a reference to the further study on asparagus preservation technologies in future.%芦笋是重要的功能型蔬菜,富含多种生物活性物质,具有很好的营养和药用功效.近年来,我国芦笋产业发展迅速,但作为鲜食为主的蔬菜,芦笋采收后高呼吸、强代谢等特点,使芦笋保鲜技术日益受到关注.综述了芦笋保鲜技术体系及影响采收后芦笋品质的机制的研究现状,并展望了其发展趋势,以期为芦笋保鲜技术深入研究提供一定的借鉴和参考.

  8. Caracteres agronómicos en el cultivo de espárrago de diferentes edades y manejos Agronomic characters in asparagus of different ages and crop systems

    Directory of Open Access Journals (Sweden)

    Pablo Diego Asprelli

    2005-01-01

    Full Text Available El objetivo del trabajo fue evaluar el efecto de la edad de la planta y la respuesta a los manejos en 11 híbridos de espárrago (Asparagus officinalis L.. Se utilizó un diseño en bloques completos aleatorios con tres repeticiones de 20 plantas por parcela, con tres y cuatro años de edad, según dos sistemas de manejo: con surcos alomados para la producción de espárrago blanco y surcos sin alomar para espárrago verde. Con el manejo para espárrago blanco se obtuvieron mayores valores promedio para días a brotación, rendimiento de mercado, rendimiento total, número de turiones y peso promedio del turión. Sin embargo, la tasa de incremento del primer al segundo año de cosecha para rendimiento de mercado y rendimiento total resultó superior en el manejo verde debido a una tasa de incremento superior para número de turiones. Para días a brotación y peso promedio del turión, la respuesta debida al efecto del manejo y de la edad del cultivo fue similar, mientras que días al 50% de parcela brotada se vio afectada principalmente por la edad de la planta.Age and crop system effects on agronomic response were evaluated in 11 hybrids of Asparagus officinalis L. The experimental design was a complete randomized block with three replicates of 20 plants of 3 or 4 years old, conducted either in mounded soil over plants for blanched asparagus, or in raised beds without ridging for green asparagus. The highest mean values were obtained under the blanched production for days to harvest, market yield, total yield, spears number and the spear mean weight. However, under the green production, the rate of increment from first to second year was superior for market yield and total yield due to an augmented rate of increment of spears number. Type and year of production effects were similar for days to first harvest and spears mean weight, meanwhile days to 50% of sprout plot was affected mainly by the year of production.

  9. Lipid lowering and antioxidant potential of Asparagus racemosus in hyperlipidemic rats

    Directory of Open Access Journals (Sweden)

    Rama R. Bhosale

    2012-06-01

    Full Text Available Background: Atherosclerosis is associated with hyperlipidemia which is a major risk factor for coronary artery disease. Therefore, treatment of hyperlipidemia is one of the major approaches to decrease the atherogenic process. Many studies revealed that Asparagus racemosus (AR possesses hypolipidemic and antioxidant potential, but results were not consistent. Therefore, the present study was undertaken to investigate lipid lowering and antioxidant potential of AR root powder in hyperlipidemic rats. Methods: Hyperlipidemia was induced in normal rats by including 0.75 gm% cholesterol and 1.5 gm% bile salt in normal diet and these rats were used for the experiments. Dried root powder of Asparagus racemosus was administered as feed supplement at 5 gm% and 10 gm% dose levels to the hyperlipidemic rats. Plasma lipid profile, malondialdehyde, ascorbic acid, catalase and superoxide dismutase were estimated using standard methods. Statistical analysis was done by one way analysis of variance (ANOVA. Results: Feed supplementation with 5 gm% and 10 gm% Asparagus racemosus resulted in a significant decrease in plasma cholesterol, LDL and significant increase in HDL. But there were no significant decrease in triglycerides and VLDL. The feed supplementation increased activities of catalase, superoxide dismutase and ascorbic acid content increased significantly in both the experimental groups (5 and 10 gm% supplemented groups. But there was no significant change in the concentration of malondialdehyde in these groups. Conclusions: The present study demonstrated that addition of Asparagus racemosus root powder at 5 gm% and 10 gm% level as feed supplement reduces the plasma lipid levels and also acts as an antioxidant. [Int J Basic Clin Pharmacol 2012; 1(3.000: 168-173

  10. A New Furost-20(22)-ene Oligoglycoside from Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-β- D-glucopyranosyl-(25S)-5β-furost-20(22)-en-3α,26-diol-3-O-α-L-rhamnopyranosyl-(1→4)-β-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.

  11. Radioprotective effects of Asparagus racemosus extracts against free radical damage in rat liver mitochondria

    International Nuclear Information System (INIS)

    The possible antioxidant effect of the extracts of Asparagus racemosus against membrane damage induced by free radicals generated during γ-radiation was examined in rat liver/brain mitochondria. These extracts displayed significant antioxidant properties in mitochondria against oxidation of both lipids and proteins as assessed by lipid peroxidation, protein oxidation and depletion of thiols. The inhibitory effect of the extracts, rich in polysaccharides like galactose, was more than that of the established antioxidants glutathione and ascorbic acid. (author)

  12. Natural variation and gene regulatory basis for the responses of asparagus beans to soil drought

    Directory of Open Access Journals (Sweden)

    Pei eXu

    2015-10-01

    Full Text Available Asparagus bean (Vigna unguiculata ssp. sesquipedalis is the Asian subspecies of cowpea, a drought-resistant legume crop native to Africa. In order to explore the genetic variation of drought responses in asparagus bean, we conducted multi-year phenotyping of drought resistance traits across the Chinese asparagus bean mini-core. The phenotypic distribution indicated that the ssp. sesquipedalis subgene pool has maintained high natural variation in drought responses despite known domestic bottleneck. Thirty-nine SNP loci were found to show an association with drought resistance via a genome-wide association study (GWAS. Whole-plant water relations were compared among four genotypes by lysimetric assay. Apparent genotypic differences in transpiration patterns and the critical soil water threshold in relation to dehydration avoidance were observed, indicating a delicate adaptive mechanism for each genotype to its own climate. Microarray gene expression analyses revealed that known drought resistance pathways such as the ABA and phosphate lipid signaling pathways are conserved between genotypes, while differential regulation of certain aquaporin genes and hormonal genes may be important for the genotypic differences. Our results suggest that divergent sensitivity to soil water content is an important mechanism configuring the genotypic specific responses to water deficit. The SNP markers identified provide useful resources for marker-assisted breeding.

  13. 中药天冬研究进展%Research Progress of Chinese Medicine Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    韦树根; 马小军; 柯芳; 黄宝优; 付金娥

    2011-01-01

    Asparagus cochinchinensis (Lour.) Merr was a kind of perennial liana plant from Asparagus Linn, of liliaceae and was one of the traditional Chinese medicine. The study on resource distribution, chemical composition, pharmacological activi-ty and clinical applications of A. Cochinchinensis in last decade was summarized. It provided a reference for the further de-velopment of A. Cochinchinensis.%天冬[Asparagus cochinchinensis (Lour.)Merr]为百合科天门冬属藤本植物,是中国传统的常用大宗中药材之一.文章分析了近十几年来国内外对天冬的研究概况,对其资源分布、化学成分、药理活性及临床应用等方面的进展进行了阐述,为天冬的进一步开发利用研究提供参考依据.

  14. ACTIVITY OF LEAF EXTRACTS OF COIX LACHRYMA LINN. AND ASPARAGUS COCHINCHINENSISLINN. AS BREAST ANTICANCER DRUGS

    Directory of Open Access Journals (Sweden)

    RESMI MUSTARICHIE

    2011-11-01

    Full Text Available In the current economic crisis, the use of plant medicine forcancer prevention should be investigated. Coix lachryma Linn and Asparagus cochinchinensis Linn are among eleven of species of medicinal plants that are noted as plant medicine for cancer in Indonesia, although their mechanism of action are still unknown. The eleven plants were screened using in vitro methods, Sulforhodamin B against breast cancer cells (MCF"7 and skin (KB. The research included a maceration process using ethanol as solvent and an anti"cancer testing process in vitro using Sulforhodamin B indicated by the value of percentage viability. Extracts were classed as being 'active anticancer' if they showed IC50 values below 100 ppm.. Coix lachryma Linn. and Asparagus cochinchinensis Linn. show breast and skin anticancer activity withIC50 values 6.51 ppm and 11.3 ppm of MCF"7 cells. The ethanol plant extracts were further extracted using various solvents with increasing polarity: n"hexane, methylene chloride, and ethyl acetate. The methylene chloride extract of Coix lachryma Linn. had IC50 = 2.75 ppm against MCF"7 cells. Against KB cells, methylene chloride extracts of Coix lachryma Linn. gave IC50 = 5.16 ppm. For Asparagus cochinchinensis Linn., an ethyl acetate extract had IC50 = 3.70 ppm against KB cancer cells and IC50 = 9.80 ppm against MCF"7 cancer cells. These data indicated that both plants can be used as anticancer drugs on breast and skin cancers.

  15. Natural variation and gene regulatory basis for the responses of asparagus beans to soil drought.

    Science.gov (United States)

    Xu, Pei; Moshelion, Menachem; Wu, XiaoHua; Halperin, Ofer; Wang, BaoGen; Luo, Jie; Wallach, Rony; Wu, Xinyi; Lu, Zhongfu; Li, Guojing

    2015-01-01

    Asparagus bean (Vigna unguiculata ssp. sesquipedalis) is the Asian subspecies of cowpea, a drought-resistant legume crop native to Africa. In order to explore the genetic variation of drought responses in asparagus bean, we conducted multi-year phenotyping of drought resistance traits across the Chinese asparagus bean mini-core. The phenotypic distribution indicated that the ssp. sesquipedalis subgene pool has maintained high natural variation in drought responses despite known domestic bottleneck. Thirty-nine SNP loci were found to show an association with drought resistance via a genome-wide association study (GWAS). Whole-plant water relations were compared among four genotypes by lysimetric assay. Apparent genotypic differences in transpiration patterns and the critical soil water threshold in relation to dehydration avoidance were observed, indicating a delicate adaptive mechanism for each genotype to its own climate. Microarray gene expression analyses revealed that known drought resistance pathways such as the ABA and phosphate lipid signaling pathways are conserved between different genotypes, while differential regulation of certain aquaporin genes and hormonal genes may be important for the genotypic differences. Our results suggest that divergent sensitivity to soil water content is an important mechanism configuring the genotypic specific responses to water deficit. The SNP markers identified provide useful resources for marker-assisted breeding. PMID:26579145

  16. 芦笋MAP保鲜研究%Modified Atmosphere Packaging of Asparagus(Asparagus officinalis L.)

    Institute of Scientific and Technical Information of China (English)

    黄光荣; 沈莲清; 王向阳

    2000-01-01

    对芦笋MAP贮藏保鲜进行了研究,结果表明,初始气体浓度比例对失重影响不明显,而对感官评价值、VC含量、纤维素含量、叶绿素含量等影响较大.温度对芦笋MAP贮藏的品质影响很大,特别是当温度超过5℃时,这种影响更为明显.对六种不同薄膜的研究表明,以低密度聚乙烯(LDPE)MAP保鲜效果最好.在各种初始气体浓度比例中以5%O2+5%CO2和10%O2+10%CP2的MAP贮藏保鲜效果最好,5℃下贮藏18d后仍有较好的品质.

  17. 我国芦笋育种研究进展%Research Progress in Asparagus Breeding in China

    Institute of Scientific and Technical Information of China (English)

    张天翔; 林宗铿; 蔡坤秀; 杨俊杰

    2011-01-01

    概述了近年来我国芦笋育种中取得的主要研究进展,介绍了芦笋在选择育种、杂交育种、多倍体育种、全雄育种、航天诱变育种等方面的研究成果,并提出了我国芦笋育种的前景和发展方向.%This paper summarized the main research progresses of asparagus breeding in China in recent years, including the research achievements in selective breeding, hybrid breeding, polyploid breeding, supermale breeding and space mutation breeding of asparagus.In addition, the author discussed the prospects and developmental direction of asparagus breeding in China in the future.

  18. 白芦笋EMAP贮藏保鲜技术研究%Studies on Equilibrium Modified Atmosphere Packaging Technology for White Asparagus

    Institute of Scientific and Technical Information of China (English)

    刘萌; 姜齐永; 郭风军; 王美兰; 周志才

    2011-01-01

    依据气调袋装白芦笋的呼吸作用和薄膜的渗气作用在一定条件下达到动态平衡的原理,研究了自发气调保鲜袋(EMAP)的面积、厚度、贮藏质量与袋内O2、CO2的关系.在温度为(3.0±0.5)℃、相对湿度90%~95%贮藏条件下,测定白芦笋贮藏前后的营养和品质指标,确定了白芦笋EMPA贮藏的适宜气体组成为:CO2,5.9%±0.3%;O2,4.2%±0.4%.利用正交试验和多元回归的方法,得出EMAP气体含量与薄膜厚度、薄膜面积、贮藏质量的回归方程,从而设计出用于白芦笋长期贮藏的EMAP.%Based on the principle of dynamic balance between the respiration of white asparagus ( Asparagus officinalis L. ) and permeability of the packaging film under a certain condition, the relationship between the 3 parameters (film area, film thickness and fruit storage weight ) and the atmosphere ( O2 and CO2 ) in EMAP was studied. The optimum atmosphere composition in EMAP that stored white asparagus at ( 3 ± 0.5 ) ℃, RH 90 %-95 % was determined through quality indicators and sensory evaluation at the end of the storage period. The optimum atmosphere composition was CO2:( 5.9 % ± 0.3 % ), O2: ( 4.2 % ± 0.4 % ). Utilizing orthogonal experiment and multiple regression method,the EMAP for storing white asparagus has the advantages of low cost, simple operation, and good effect.

  19. 芦笋酒发酵工艺参数的优化研究%Optimize the Technical Parameters of Asparagus Wine Fermenting

    Institute of Scientific and Technical Information of China (English)

    常学东; 侍朋宝; 孟军; 张建才; 刘颜龙

    2011-01-01

    [Objective] The aim was to obtain the suitable technical parameters of asparagus wine fermenting. [ Method] Three kinds of yeasts were used to make asparagus wine, and orthogonal experimental design was used to study the influential factors of asparagus wine. [ Result] The suitable yeast for making asparagus wine was danbaoli yeast, and the optimal ferment conditions of asparagus wine were as follows; 170 g/L the sugar content, 7 U/g proteinase dosage and citric acid dosage was 2 g/L. [ Conclusion] The research provided reference for development and utilization of leftover parts of asparagus in aspect of making asparagus wine.%[目的]研究芦笋酒发酵最适工艺参数.[方法]采用3种酵母对芦笋汁进行发酵,再以正交试验设计对芦笋酒的发酵影响因素进行研究.[结果]丹宝利酵母最适于芦笋酒的发酵,且最佳工艺参数为糖度170 g/L,蛋白酶用量7 U/g,柠檬酸用量为2 g/L.[结论]该研究为芦笋下脚料在酿酒方面的开发利用提供了依据.

  20. PELADO QUIMICO Y TERMOFISICO DE ESPARRAGOS CHEMICAL AND STEAM PEELING OF ASPARAGUS

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    Raúl L. GARROTE

    1998-08-01

    Full Text Available Se ha estudiado el pelado químico y termofísico en una etapa de espárragos de la variedad Argentuil. Para el pelado químico se utilizó la Metodología de la Superficie de Respuesta, evaluando el efecto que las variables concentración del baño de NaOH (20, 40, 60 g/kg, temperatura del baño (55, 70, 85ºC y tiempo de pelado (30, 75, 120 segundos tienen sobre el rendimiento de la operación y la calidad del pelado. Para el pelado termofísico se estudió el efecto que el tiempo de pelado (10, 20, 30 segundos y el tamaño del espárrago ejercen sobre las respuestas mencionadas. Se determinó que los modelos estadísticos obtenidos para el pelado químico fueron apropiados, encontrándose que la mejor calidad de pelado y más altos rendimientos se ubican para condiciones de baja concentración de NaOH, corto tiempo y alta temperatura. En el pelado termofísico el tiempo óptimo de procesamiento, para ambos tamaños de espárragos, fue de 20 segundos, para una presión de trabajo de 5,1 kg/m2.One stage chemical and steam peeling of asparagus, Argentuil variety, has been studied. Response Surface Methodology was used to evaluate the effect of the variables, NaOH concentration of bath (20, 40, 60 g/kg, bath temperature (55, 70, 85ºC and the peeling time (30, 75, 120 seconds on yield and peeling quality. For steam peeling process the effect of peeling time (10, 20, 30 seconds and asparagus size on the mentioned responses was also studied. Statistical models developed for chemical peeling of asparagus were appropriate; processing conditions for optimum peeling quality and highest yield were found in regions of low NaOH bath concentration, short peeling time and high bath temperature. The optimum steam peeling time, for both asparagus sizes, was 20 seconds, for a steam pressure of 5.1 kg/cm2.

  1. EFFECT OF FEEDS SUPPLEMENTED WITH ASPARAGUS RACEMOSUS ON MILK PRODUCTION OF INDIGENOUS COWS

    Directory of Open Access Journals (Sweden)

    KIRAN KUMAR DIVYA

    2015-03-01

    Full Text Available Indigenous cows, on farm level, kept on feeding supplemented with powdered root of Shatavari (Asparagus racemosus Willd.. It was observed that feeding Shatavari roots supplemented feed increased percentage milk yield, fat, solid not fat (SNF and total solids significantly without altering quality and natural attributes of milk. Significant residual effect of feeding Shatavari roots supplemented feed was also observed in terms of percentage increase in milk yield, fat, solid not fat (SNF and total solids (TS up to ten days after Shatavari roots supplemented was stopped to cows.

  2. Anatomical observation on the developmental stages of Asparagus of ficinalis L.staminate and pistillate flowers%芦笋雌雄花发育过程的解剖学观察

    Institute of Scientific and Technical Information of China (English)

    胡淑明; 陈海媛; 王海燕; 乜兰春

    2013-01-01

    To study and ascertain the relations among Asparagus officinalis L. bisexual flowers in duction, differential gene segregation critical period and bud size, paraffin section method was applied to observe Asparagus flower buds of different diameter morphologically. The results showed that stamens and carpel are initiated in both staminate and pistillate flowers in prophase. However, as flowers developing, stamens and carpel stopped growth both in male and female individuals. It's the critical period for male Asparagus's bud when pistillate organs in it stopped developing and it's diameter was 2. 0 millimeters. The results are referential for inducing male bisexual flowers and choosing differential gene segregation time of sex differentiation.%为了研究并确定芦笋(Asparagus of ficinalis L.)两性完全花诱导和差异基因分离的关键时期与花蕾大小的关系,采用石蜡切片技术,对不同直径芦笋花蕾的性别分化过程进行了形态学观察.结果表明:在雄株和雌株的花器官发育早期,两者都有雄蕊和心皮.在随后的发育过程中,雄蕊和心皮分别在雌性和雄性个体中停止发育.雄花花蕾直径2.0 mm,是芦笋雄花雌性器官发育停滞的关键时期,为雄性两性完全花诱导和性别分化差异基因分离时期的选择提供了参考.

  3. STUDY ON CO2 SUPERCRITICAL FLUID EXTRACTION OF URSOLIC ACID FROM ASPARAGUS%CO2超临界流体萃取芦笋中熊果酸的研究

    Institute of Scientific and Technical Information of China (English)

    崔星明; 王勇为; 陈光宇

    2004-01-01

    用CO2超临界流体萃取得到的芦笋(Asparagus officinalis L.)提取物,用甲醇抽提醇溶部分,经用液质联用仪检测,得到56个紫外检测峰.其中,有保留时间与熊果酸基本一致的峰.其质谱的分子离子峰及特征碎片峰也与熊果酸的一致.确定该化合物为熊果酸.

  4. Viabilidad económica de la producción de espárrago en la Región del Maule, Chile Economic viability of asparagus production in the Maule Region, Chile

    OpenAIRE

    Germán L Andrade; Tristán M Ibáñez

    2006-01-01

    En esta investigación se estimaron indicadores de viabilidad y de eficiencia económica de la producción de espárrago (Asparagus officinalis L.) en Chile. Los coeficientes técnicos fueron tomados de los registros históricos de un predio localizado en la zona de Linares, Región del Maule, Chile. Los indicadores de viabilidad calculados fueron el valor actual neto (VAN), tasa interna de retorno (TIR), índice de valor actual neto (IVAN), razón beneficio-costo (RBC), y período de recuperación del ...

  5. Steam Cooking Significantly Improves in Vitro Bile Acid Binding of Beets, Eggplant, Asparagus, Carrots, Green Beans and Cauliflower

    Science.gov (United States)

    The relative healthful potential of cooked beets, okra, eggplant, asparagus, carrots, green beans, cauliflower and turnips was evaluated by determining their in vitro bile acid binding using a mixture of bile acids secreted in human bile at a duodenal physiological pH of 6.3. Six treatments and two...

  6. Pure phosphine fumigation treatment at low temperature for postharvest control of western flower thrips on lettuce, broccoli, asparagus, and strawberries

    Science.gov (United States)

    U.S. exported lettuce, broccoli, asparagus, and strawberries often harbor western flower thrips (Frankliniella occidentalis), a quarantined pest in Taiwan, and therefore require quarantine treatment. Pure phosphine fumigation at a low temperature of 2°C was studied as an alternative fumigant to meth...

  7. A new pest species of Copitarsia Hampson from the Neotropical Region feeding on Asparagus and cut flowers (Lepidoptera: Noctuidae)

    Science.gov (United States)

    The egg, first- and last- instar larva, and adult of Copitarsia corruda, n. sp. from Mexico, Colombia, Ecuador, and Peru are described and illustrated. Larval host-plant genera include Asparagus (Liliaceae) (Mexico, Colombia, Ecuador), Iris (Iridaceae) (Ecuador), Ammi (Apiaceae) (Ecuador), Lysimachi...

  8. Analysis of SSR information in EST resource of asparagus%芦笋EST资源的SSR信息分析

    Institute of Scientific and Technical Information of China (English)

    吴建霞; 李亚玲; 张智俊; 管雨

    2012-01-01

    To develop asparagus EST -SSRs function of molecular marker, Simple sequence repeats!(SSRs) were investigated in 8590 qualified ESTs downloaded from Nucleotide ESTs database of Lentinula edodes in NCBI . After eliminated redundancy, we got 8377 sequence of nonredundancy, in which there were 469 EST - SSRs, the mean distance was 14.80 kb in non - redundant ESTs. Of the total EST - SSRs, 40.51% (190/469 ) were dinucleotides repeats(DNRs) ,34.79% (164/469) were trinucleotide repeats (TNRs) and 21.11% (99/469) were hexanucle-otide repeats (HNRs). In which the repeat motifs CT/AG were the most abundant up to 13. 22% (62/469). Random filter out 30 EST - SSR sequences, then using primer5 software designed its primers for PCR amplification. 27 of primers showed amplifications, in which 21 pairs could amplify distinct bands ,accounted for 80% (24/30). Al-leles in asparagus were abundant , average 4. 93 each pair. These EST - SSR markers will be useful in studies of population genetics, linkage mapping, molecular mapping, gene tagging, and pedigree analysis and other aspects for this species.%为了在芦笋中开发EST - SSR功能性标记,对来源于NCBI公共数据库的8 590条芦笋(Asparagus officinalis L.)EST序列进行简单重复序列SSR搜索.剔除冗余序列,得到非冗余序列8 377条.在非冗余序列中共挖掘出469个EST - SSR,平均相隔14.80 kb出现1个SSR.在所有的重复基序中,二核苷酸重复基序的SSR所占比例最高40.51% (190/469),其次是三核苷酸34.97% (164/469),六核苷酸21.11%(99/469).在所有基序里,CT/AG出现的频率最高有62次,占全部重复基序的13.22% (62/469).选取含SSR的EST序列30条,并利用primer5软件设计引物,进行SSR位点的扩增,其中27对引物扩增产物,24对有较清晰可靠的目标扩增条带,占引物数的80%,且所检测出的芦笋等位基因数量较丰富,平均4.93个/对.这些EST - SSR标记的开发将有助于芦笋群体遗传多样性、遗传图谱

  9. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress.

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic engineering, and

  10. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars—“Dubai bean” and “Ningjiang-3”, which are tolerant and sensitive to chilling, respectively—were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic

  11. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis) Cultivars Differing in Chilling Tolerance under Cold Stress.

    Science.gov (United States)

    Tan, Huaqiang; Huang, Haitao; Tie, Manman; Tang, Yi; Lai, Yunsong; Li, Huanxiu

    2016-01-01

    Cowpea (V. unguiculata L. Walp.) is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis) is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18%) had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs) in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic engineering, and

  12. Transcriptome Profiling of Two Asparagus Bean (Vigna unguiculata subsp. sesquipedalis Cultivars Differing in Chilling Tolerance under Cold Stress.

    Directory of Open Access Journals (Sweden)

    Huaqiang Tan

    Full Text Available Cowpea (V. unguiculata L. Walp. is an important tropical grain legume. Asparagus bean (V. unguiculata ssp. sesquipedialis is a distinctive subspecies of cowpea, which is considered one of the top ten Asian vegetables. It can be adapted to a wide range of environmental stimuli such as drought and heat. Nevertheless, it is an extremely cold-sensitive tropical species. Improvement of chilling tolerance in asparagus bean may significantly increase its production and prolong its supply. However, gene regulation and signaling pathways related to cold response in this crop remain unknown. Using Illumina sequencing technology, modification of global gene expression in response to chilling stress in two asparagus bean cultivars-"Dubai bean" and "Ningjiang-3", which are tolerant and sensitive to chilling, respectively-were investigated. More than 1.8 million clean reads were obtained from each sample. After de novo assembly, 88,869 unigenes were finally generated with a mean length of 635 bp. Of these unigenes, 41,925 (47.18% had functional annotations when aligned to public protein databases. Further, we identified 3,510 differentially expressed genes (DEGs in Dubai bean, including 2,103 up-regulated genes and 1,407 down-regulated genes. While in Ningjiang-3, we found 2,868 DEGs, 1,786 of which were increasing and the others were decreasing. 1,744 DEGs were commonly regulated in two cultivars, suggesting that some genes play fundamental roles in asparagus bean during cold stress. Functional classification of the DEGs in two cultivars using Mercator pipeline indicated that RNA, protein, signaling, stress and hormone metabolism were five major groups. In RNA group, analysis of TFs in DREB subfamily showed that ICE1-CBF3-COR cold responsive cascade may also exist in asparagus bean. Our study is the first to provide the transcriptome sequence resource for asparagus bean, which will accelerate breeding cold resistant asparagus bean varieties through genetic

  13. PRELIMINARY PHYTOCHEMICAL AND INVITRO CYTOTOXIC ACTIVITY OF THE LEAVES OF Asparagus racemosus Willd., (Liliaceae

    Directory of Open Access Journals (Sweden)

    K. Durai Prabakaran

    2015-04-01

    Full Text Available Asparagus racemosus Willd.(Shatavari is a well known plant in Ayurvedic systems of medicine. ‘Shatavari’ is a reputed classical drug and said to possess therapeutic properties as Rasayana drugs of Ayurveda. The present study is aimed at the development of phytochemical parameters and invitro cytotoxic activity of various extracts of the leaves of Asparagus racemosus plant. The plant material was successively extracted with solvents of increasing polarity namely n-Hexane, chloroform, ethyl acetate and ethanol in a soxhlet extractor. Preliminary phytochemical tests, fluorescence analysis, thin layer chromatography and high performance thin layer chromatographic studies were carried out for all the extracts. Chloroform, ethyl acetate and ethanol extracts were screened for invitro cytotoxic activity by MTT assay using EAC (Ehrlich’s Ascites Carcinoma cell line. The preliminary phytochemical analysis of various extracts of the leaves showed the presence of carbohydrates, tannins, phenolic compounds, sterols and flavonoids. The chloroform and ethyl acetate extracts showed significant cytotoxic activity when compared to ethanol extract against the above mentioned cancer cell line.

  14. Application of PCR-denaturing-gradient gel electrophoresis (DGGE) method to examine microbial community structure in asparagus fields with growth inhibition due to continuous cropping.

    Science.gov (United States)

    Urashima, Yasufumi; Sonoda, Takahiro; Fujita, Yuko; Uragami, Atsuko

    2012-01-01

    Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still controversial. We aimed to develop a technique for the biological field diagnosis of growth inhibition due to continuous cropping. Therefore, in this study, fungal community structure and Fusarium community structure in continuously cropped fields of asparagus were analyzed by polymerase chain reaction/denaturing-gradient gel electrophoresis (PCR-DGGE). Soil samples were collected from the Aizu region of Fukushima Prefecture, Japan. Soil samples were taken from both continuously cropped fields of asparagus with growth inhibition and healthy neighboring fields of asparagus. The soil samples were collected from the fields of 5 sets in 2008 and 4 sets in 2009. We were able to distinguish between pathogenic and non-pathogenic Fusarium by using Alfie1 and Alfie2GC as the second PCR primers and PCR-DGGE. Fungal community structure was not greatly involved in the growth inhibition of asparagus due to continuous cropping. By contrast, the band ratios of Fusarium oxysporum f. sp. asparagi in growth-inhibited fields were higher than those in neighboring healthy fields. In addition, there was a positive correlation between the band ratios of Fusarium oxysporum f. sp. asparagi and the ratios of missing asparagus plants. We showed the potential of biological field diagnosis of growth inhibition due to continuous cropping of asparagus using PCR-DGGE.

  15. Methanolic extract of white asparagus shoots activates TRAIL apoptotic death pathway in human cancer cells and inhibits colon carcinogenesis in a preclinical model.

    Science.gov (United States)

    Bousserouel, Souad; Le Grandois, Julie; Gossé, Francine; Werner, Dalal; Barth, Stephan W; Marchioni, Eric; Marescaux, Jacques; Raul, Francis

    2013-08-01

    Shoots of white asparagus are a popular vegetable dish, known to be rich in many bioactive phytochemicals reported to possess antioxidant, and anti-inflammatory and antitumor activities. We evaluated the anticancer mechanisms of a methanolic extract of Asparagus officinalis L. shoots (Asp) on human colon carcinoma cells (SW480) and their derived metastatic cells (SW620), and Asp chemopreventive properties were also assessed in a model of colon carcinogenesis. SW480 and SW620 cell proliferation was inhibited by 80% after exposure to Asp (80 µg/ml). We demonstrated that Asp induced cell death through the activation of TRAIL DR4/DR5 death receptors leading to the activation of caspase-8 and caspase-3 and to cell apoptosis. By specific blocking agents of DR4/DR5 receptors we were able to prevent Asp-triggered cell death confirming the key role of DR4/DR5 receptors. We found also that Asp (80 µg/ml) was able to potentiate the effects of the cytokine TRAIL on cell death even in the TRAIL-resistant metastatic SW620 cells. Colon carcinogenesis was initiated in Wistar rats by intraperitoneal injections of azoxymethane (AOM), once a week for two weeks. One week after (post-initiation) rats received daily Asp (0.01%, 14 mg/kg body weight) in drinking water. After 7 weeks of Asp-treatment the colon of rats exhibited a 50% reduction of the number of preneoplastic lesions (aberrant crypt foci). In addition Asp induced inhibition of several pro-inflammatory mediators, in association with an increased expression of host-defense mediators. In the colonic mucosa of Asp-treated rats we also confirmed the pro-apoptotic effects observed in vitro including the activation of the TRAIL death‑receptor signaling pathway. Taken together, our data highlight the chemopreventive effects of Asp on colon carcinogenesis and its ability to promote normal cellular homeostasis.

  16. Asparagus IRX9, IRX10, and IRX14A Are Components of an Active Xylan Backbone Synthase Complex that Forms in the Golgi Apparatus1[OPEN

    Science.gov (United States)

    Zeng, Wei; Picard, Kelsey L.; Song, Lili; Wu, Ai-Min; Farion, Isabela M.; Zhao, Jia; Ford, Kris; Bacic, Antony

    2016-01-01

    Heteroxylans are abundant components of plant cell walls and provide important raw materials for the food, pharmaceutical, and biofuel industries. A number of studies in Arabidopsis (Arabidopsis thaliana) have suggested that the IRREGULAR XYLEM9 (IRX9), IRX10, and IRX14 proteins, as well as their homologs, are involved in xylan synthesis via a Golgi-localized complex termed the xylan synthase complex (XSC). However, both the biochemical and cell biological research lags the genetic and molecular evidence. In this study, we characterized garden asparagus (Asparagus officinalis) stem xylan biosynthesis genes (AoIRX9, AoIRX9L, AoIRX10, AoIRX14A, and AoIRX14B) by heterologous expression in Nicotiana benthamiana. We reconstituted and partially purified an active XSC and showed that three proteins, AoIRX9, AoIRX10, and AoIRX14A, are necessary for xylan xylosyltranferase activity in planta. To better understand the XSC structure and its composition, we carried out coimmunoprecipitation and bimolecular fluorescence complementation analysis to show the molecular interactions between these three IRX proteins. Using a site-directed mutagenesis approach, we showed that the DxD motifs of AoIRX10 and AoIRX14A are crucial for the catalytic activity. These data provide, to our knowledge, the first lines of biochemical and cell biological evidence that AoIRX9, AoIRX10, and AoIRX14A are core components of a Golgi-localized XSC, each with distinct roles for effective heteroxylan biosynthesis. PMID:26951434

  17. Asparagus IRX9, IRX10, and IRX14A Are Components of an Active Xylan Backbone Synthase Complex that Forms in the Golgi Apparatus.

    Science.gov (United States)

    Zeng, Wei; Lampugnani, Edwin R; Picard, Kelsey L; Song, Lili; Wu, Ai-Min; Farion, Isabela M; Zhao, Jia; Ford, Kris; Doblin, Monika S; Bacic, Antony

    2016-05-01

    Heteroxylans are abundant components of plant cell walls and provide important raw materials for the food, pharmaceutical, and biofuel industries. A number of studies in Arabidopsis (Arabidopsis thaliana) have suggested that the IRREGULAR XYLEM9 (IRX9), IRX10, and IRX14 proteins, as well as their homologs, are involved in xylan synthesis via a Golgi-localized complex termed the xylan synthase complex (XSC). However, both the biochemical and cell biological research lags the genetic and molecular evidence. In this study, we characterized garden asparagus (Asparagus officinalis) stem xylan biosynthesis genes (AoIRX9, AoIRX9L, AoIRX10, AoIRX14A, and AoIRX14B) by heterologous expression in Nicotiana benthamiana We reconstituted and partially purified an active XSC and showed that three proteins, AoIRX9, AoIRX10, and AoIRX14A, are necessary for xylan xylosyltranferase activity in planta. To better understand the XSC structure and its composition, we carried out coimmunoprecipitation and bimolecular fluorescence complementation analysis to show the molecular interactions between these three IRX proteins. Using a site-directed mutagenesis approach, we showed that the DxD motifs of AoIRX10 and AoIRX14A are crucial for the catalytic activity. These data provide, to our knowledge, the first lines of biochemical and cell biological evidence that AoIRX9, AoIRX10, and AoIRX14A are core components of a Golgi-localized XSC, each with distinct roles for effective heteroxylan biosynthesis. PMID:26951434

  18. Methanolic extract of white asparagus shoots activates TRAIL apoptotic death pathway in human cancer cells and inhibits colon carcinogenesis in a preclinical model.

    Science.gov (United States)

    Bousserouel, Souad; Le Grandois, Julie; Gossé, Francine; Werner, Dalal; Barth, Stephan W; Marchioni, Eric; Marescaux, Jacques; Raul, Francis

    2013-08-01

    Shoots of white asparagus are a popular vegetable dish, known to be rich in many bioactive phytochemicals reported to possess antioxidant, and anti-inflammatory and antitumor activities. We evaluated the anticancer mechanisms of a methanolic extract of Asparagus officinalis L. shoots (Asp) on human colon carcinoma cells (SW480) and their derived metastatic cells (SW620), and Asp chemopreventive properties were also assessed in a model of colon carcinogenesis. SW480 and SW620 cell proliferation was inhibited by 80% after exposure to Asp (80 µg/ml). We demonstrated that Asp induced cell death through the activation of TRAIL DR4/DR5 death receptors leading to the activation of caspase-8 and caspase-3 and to cell apoptosis. By specific blocking agents of DR4/DR5 receptors we were able to prevent Asp-triggered cell death confirming the key role of DR4/DR5 receptors. We found also that Asp (80 µg/ml) was able to potentiate the effects of the cytokine TRAIL on cell death even in the TRAIL-resistant metastatic SW620 cells. Colon carcinogenesis was initiated in Wistar rats by intraperitoneal injections of azoxymethane (AOM), once a week for two weeks. One week after (post-initiation) rats received daily Asp (0.01%, 14 mg/kg body weight) in drinking water. After 7 weeks of Asp-treatment the colon of rats exhibited a 50% reduction of the number of preneoplastic lesions (aberrant crypt foci). In addition Asp induced inhibition of several pro-inflammatory mediators, in association with an increased expression of host-defense mediators. In the colonic mucosa of Asp-treated rats we also confirmed the pro-apoptotic effects observed in vitro including the activation of the TRAIL death‑receptor signaling pathway. Taken together, our data highlight the chemopreventive effects of Asp on colon carcinogenesis and its ability to promote normal cellular homeostasis. PMID:23754197

  19. Complete genome sequences of two biologically distinct isolates of Asparagus virus 1.

    Science.gov (United States)

    Blockus, S; Lesker, T; Maiss, E

    2015-02-01

    The complete genome sequences of two asparagus virus 1 (AV-1) isolates differing in their ability to cause systemic infection in Nicotiana benthamiana were determined. Their genomes had 9,741 nucleotides excluding the 3'-terminal poly(A) tail, encoded a polyprotein of 3,112 amino acids, and shared 99.6 % nucleotide sequence identity. They differed at 37 nucleotide and 15 amino acid sequence positions (99.5 % identity) scattered over the polyprotein. The closest relatives of AV-1 in amino acid sequence identity were plum pox virus (54 %) and turnip mosaic virus (53 %), corroborating the classification of AV-1 as a member of a distinct species in the genus Potyvirus.

  20. Sustainability of Global and Local Food Value Chains: An Empirical Comparison of Peruvian and Belgian Asparagus

    Directory of Open Access Journals (Sweden)

    Jana Schwarz

    2016-04-01

    Full Text Available The sustainability of food value chains is an increasing concern for consumers, food companies and policy-makers. Global food chains are often perceived to be less sustainable than local food chains. Yet, thorough food chain analyses and comparisons of different food chains across sustainability dimensions are rare. In this article we analyze the local Belgian and global Peruvian asparagus value chains and explore their sustainability performance. A range of indicators linked to environmental, economic and social impacts is calculated to analyze the contribution of the supply chains to economic development, resource use, labor relations, distribution of added value and governance issues. Our findings suggest that none of the two supply chains performs invariably better and that there are trade-offs among and between sustainability dimensions. Whereas the global chain uses water and other inputs more intensively and generates more employment per unit of land and higher yields, the local chain generates more revenue per unit of land.

  1. Innovative Approach to the Accumulation of Rubrosterone by Fermentation of Asparagus filicinus with Fusarium oxysporum.

    Science.gov (United States)

    Li, Ying; Cai, Le; Dong, Jian-Wei; Xing, Yun; Duan, Wei-He; Zhou, Hao; Ding, Zhong-Tao

    2015-07-29

    Rubrosterone, possessing various remarkable bioactivities, is an insect-molting C19-steroid. However, only very small amounts are available for biological tests due to its limited content from plant sources. Fungi of genus Fusarium have been reported to have the ability to convert C27-steroids into C19-steroids. In this study, Asparagus filicinus, containing a high content of 20-hydroxyecdysone, was utilized to accumulate rubrosterone through solid fermentation by Fusarium oxysporum. The results showed that F. oxysporum had the ability to facilitate the complete biotransformation of 20-hydroxyecdysone to rubrosterone by solid-state fermentation. The present method could be an innovative and efficient approach to accumulate rubrosterone with an outstanding conversion ratio.

  2. REVERSAL EFFECT OF ASPARAGUS RACEMOSUS WILD (LILIACEAE ROOT EXTRACT ON MEMORY DEFICITS OF MICE

    Directory of Open Access Journals (Sweden)

    Ashwlayan Vrish Dhwaj

    2011-06-01

    Full Text Available Methanolic extract of roots of Asparagus racemosus wild (Liliaceae was investigated for its reversal effect on memory deficits in mice. Two doses the extract (75 and 150 mg/kg, i.p. were administered for seven consecutive days. Scopolamine (0.4 mg/kg, i.p., sodium nitrite (75 mg/kg, i.p. were used to induce memory deficits (amnesia. Elevated plus maze (EPM and Morris water maze (MWM were employed to evaluate short and long term memory respectively. Scopolamine and sodium nitrite treatment produced significant impairment of elevated plus maze and Morris water maze performance indicating impairment of memory. The methanolic root extract (150 mg/kg, i.p. significantly (p<0.05 improved EPM and MWM performance of scopolamine and sodium nitrite treated mice. The results indicated potential of the plant in relieving memory deficits.

  3. Suitability Of Sixteen Asparagus Cultivars For Growing In Polish Environmental Conditions

    Directory of Open Access Journals (Sweden)

    Knaflewski Mikołaj

    2014-12-01

    Full Text Available Green spear yield of 16 asparagus cultivars originating from six countries was assessed from 2011 to 2013. There were considerable differences in total, marketable and early yields between the cultivars. The quality of harvested spears also varied remarkably. On average, the highest mean total and marketable yields from 3 years of investigation came from Dutch cultivars ‘Gijnlim’ and ‘Cumulus’ and German cv. ‘Mondeo’. The lowest yields were found in New Zealand cultivars ‘Pacific Challenger’ and ‘Pacific 2000’. The greatest mean marketable spear weight was recorded for ‘Cumulus’, whereas the smallest spear weight was obtained from ‘Pacific 2000’, ‘H666’ and ‘NJ 953’. Cultivars ‘Gijnlim’, ‘Cumulus’ and ‘Mondeo’ produced the highest early yields, while ‘Pacific 2000’ and ‘Pacific Challenger’ produced the lowest.

  4. Innovative Approach to the Accumulation of Rubrosterone by Fermentation of Asparagus filicinus with Fusarium oxysporum.

    Science.gov (United States)

    Li, Ying; Cai, Le; Dong, Jian-Wei; Xing, Yun; Duan, Wei-He; Zhou, Hao; Ding, Zhong-Tao

    2015-07-29

    Rubrosterone, possessing various remarkable bioactivities, is an insect-molting C19-steroid. However, only very small amounts are available for biological tests due to its limited content from plant sources. Fungi of genus Fusarium have been reported to have the ability to convert C27-steroids into C19-steroids. In this study, Asparagus filicinus, containing a high content of 20-hydroxyecdysone, was utilized to accumulate rubrosterone through solid fermentation by Fusarium oxysporum. The results showed that F. oxysporum had the ability to facilitate the complete biotransformation of 20-hydroxyecdysone to rubrosterone by solid-state fermentation. The present method could be an innovative and efficient approach to accumulate rubrosterone with an outstanding conversion ratio. PMID:26145461

  5. Enzyme-treated asparagus extract promotes expression of heat shock protein and exerts antistress effects.

    Science.gov (United States)

    Ito, Tomohiro; Maeda, Takahiro; Goto, Kazunori; Miura, Takehito; Wakame, Koji; Nishioka, Hiroshi; Sato, Atsuya

    2014-03-01

    A novel enzyme-treated asparagus extract (ETAS) has been developed as a functional material produced from asparagus stem. Studies were conducted to determine the effect of ETAS on heat shock protein 70 (HSP70) expression and alleviation of stress. HeLa cells were treated with ETAS, and HSP70 mRNA and protein levels were measured using a reverse transcription-polymerase chain reaction (RT-PCR) assay and an enzyme-linked immunosorbent assay (ELISA), respectively. ETAS showed significant increases in HSP70 mRNA at more than 0.125 mg/mL and the protein at more than 1.0 mg/mL. The antistress effect was evaluated in a murine sleep-deprivation model. A sleep-deprivation stress load resulted in elevation of blood corticosterone and lipid peroxide concentrations, while supplementation with ETAS at 200 and 1000 mg/kg body weight was associated with significantly reduced levels of both stress markers, which were in the normal range. The HSP70 protein expression level in mice subjected to sleep-deprivation stress and supplemented with ETAS was significantly enhanced in stomach, liver, and kidney, compared to ETAS-untreated mice. A preliminary and small-sized human study was conducted among healthy volunteers consuming up to 150 mg/d of ETAS daily for 7 d. The mRNA expression of HSP70 in peripheral leukocytes was significantly elevated at intakes of 100 or 150 mg/d, compared to their baseline levels. Since HSP70 is known to be a stress-related protein and its induction leads to cytoprotection, the present results suggest that ETAS might exert antistress effects under stressful conditions, resulting from enhancement of HSP70 expression.

  6. Viabilidad económica de la producción de espárrago en la Región del Maule, Chile Economic viability of asparagus production in the Maule Region, Chile

    Directory of Open Access Journals (Sweden)

    Germán L Andrade

    2006-06-01

    Full Text Available En esta investigación se estimaron indicadores de viabilidad y de eficiencia económica de la producción de espárrago (Asparagus officinalis L. en Chile. Los coeficientes técnicos fueron tomados de los registros históricos de un predio localizado en la zona de Linares, Región del Maule, Chile. Los indicadores de viabilidad calculados fueron el valor actual neto (VAN, tasa interna de retorno (TIR, índice de valor actual neto (IVAN, razón beneficio-costo (RBC, y período de recuperación del capital (PRC. Los indicadores de eficiencia económica fueron el costo medio total (CMET, margen unitario ponderado (MUP y la rentabilidad sobre capital (RSC. Como unidad de análisis se consideró una hectárea. Para productores con tierra, los resultados obtenidos en la situación estándar fueron: Ch$1,52 millones (VAN, 12,1% (TIR, 0,3 (IVAN, 1,1 (RBC y 10 años (PRC. Para la etapa de producción se proyectó un CMET mínimo de Ch $317,8 kg-1, un MUP máximo de Ch$407 kg-1 y una RSC de 20,3%. La principal conclusión es que el cultivo de espárrago en la región del Maule, en Chile, es viable para los productores con tierra.Viability and economic efficiency indicators for asparagus (Asparagus officinalis L. production in Chile were evaluated. Technical parameters were obtained from an historical farm located in Linares area, Maule Region, Chile. Viability was assessed by means of net present value (VAN, internal rate of return (TIR, net present value index (IVAN, cost-benefit ratio (RBC, and investment recovery period (PRC. Economic efficiency was assessed through production average cost (CMET, weighted unit margin (MUP, and return on equity (RSC. The analysis was based upon one hectare. Results for producers who own their land were US$ 2,529 ha-1 (VAN, 12.14% (TIR, 0.25 (IVAN, and 1.1 (RBC. The minimum CMET was estimated at US$ 0.53 kg-1 the maximum MUP at US$ 0.68 kg-1, and RSC until 20.3%. The main conclusion is that asparagus production in the

  7. Effects of Different Amount of Phosphorus Fertilizer on Yield and Nutrient Quality of Green Asparagus%不同磷肥用量对绿芦笋产量及营养品质的影响

    Institute of Scientific and Technical Information of China (English)

    杨恒山; 谷永丽; 张瑞富; 刘晶

    2011-01-01

    In experimental farm of Inner Mongolia University for Nationalities from March to July in 2008, using four-year Asparagus officinalis L. cv. Apollo as experimental material, the effects of different amount of phosphorus fertilizer on yield and nutrient quality of green asparagus were studied. The results showed that the yield of green asparagus increased first and then slightly decreased with the increase of the amount of phosphorus fertilizer during the harvesting time when the amount of phosphorus fertilizer was within P 0 - 90 kg ha. The weight of per branches and the increase of branches were the major reasons for the increase of the yield of green asparagus. With the increase of amount of phosphorus fertilizer, the content of chlorophyll a and b increase, and the content of carotenoid had no obvious change, and the content of chlorophyll a, chlorophyll b and carotenoid in later period was higher than those in prophase and metaphase. The content of soluble sugar, crude protein, soluble protein all increased with the increase of amount of phosphorus fertilizer. The content of mineral elements were much higher when the amount of phosphorus fertilizer was appropriate, but which was lower when the amount of phosphorus was too much or little. Under this experimental condition, when the amount of phosphorus fertilizer was about P 72 kg ha, green asparagus not only has higher yield and better nutrient quality.%2008年3~7月在西辽河平原的内蒙古民族大学实验农场,以4年生芦笋阿波罗(Asparagus officinalis L.cv.Apollo)为试材,研究了不同磷肥用量对绿芦笋产量及营养品质的影响.结果表明,施磷量在0~90kg hm-2的范围内,绿芦笋产量随施磷量的增加呈先升高后略有降低的变化趋势,单支重和支数均有不同程度的增加.随着磷肥用量的增加,叶绿素a、叶绿素h的含量增加,而类胡萝卜素的含量变化不大;采笋后期叶绿素a、叶绿素b、类胡萝卜素含量均明显地

  8. Study on HepG-2 apoptosis induced by saponins isolated from Asparagus and the effects on the activities of caspase-3,8,9

    Institute of Scientific and Technical Information of China (English)

    JI Yu-bin; XU He; JI Chen-feng

    2008-01-01

    Objective To study the effect of saponins of asparagus on apoptosis and the variations of caspaseS, caspase-9 and caspase-3 activity in the process of asparagus induced apoptosis in HepG-2, to investigate the apoptosis mechanism further. Methods Asparagus on apoptosis effects on tumor cells cultured-HepG-2 with different concentrations at different time, IC50 value was measured by MTT assay, the apoptosis rate was determined by FCM with AnnexinV/PI staining, their apoptotic morphology were observed by electron microscopy and Colorimetric method was used to measure caspase-8,9 and caspase-3 activities. Results Experiments of antitumour in vivo showed that saponins of asparagus can inhibit the growth of tumor cell of HepG-2 in evidence, IC50 was 101.15 mg·L-1. Cultured for 72 h, the apoptosis rate had positive increased with concentrations. Apoptotic morphology was observed by electron microscopy. The activities of caspase-8, easpase-9 and caspase-3 had positive increased with concentrations. And have significant difference compared with negative control group(P<0.01). The activities of caspase-8 were high at 24 h, but the activities of caspase-9 and caspase-3 is high at 48 h. Conclusions Aaponins of asparagus can inhibit the growth of tumor cell of HepG2, and the underlying mechanism might be related to up regulation of caspase-8, 9 activity which subsequently transforms caspase-3 into its active form.

  9. Asparagus chain: Fifth year production of different genotypes and postharvest behaviour.

    Directory of Open Access Journals (Sweden)

    Erika Kirschenbilder

    2015-01-01

    Full Text Available The objective of this investigation was to study greenhouse precocious spears yield of three genotypes of asparagus on its fifth harvest season. The trial was established at the Faculty of Agricultural, Pontifical Catholic University of Argentina, on November, 15, 2006. 19 different harvests were made from 04/09/2012 to 22/10/2012, one every two days. A multifactor ANOVA-LSD test (P>0.05 was performed. Variables analyzed were: total productivity and commercial productivity (PFT and PFC, total number of spears and commercial number of spears (NTT and NTC; caliber distribution (DC: Jumbo (J, Extra-Large (XL, Large (L, Medium (M and Small (S and finally, quality defects (D: spiked spears (TE; short spears (TC and other defects (OD. During the post-harvest period, bundles (A, trays (BA and polyethylene bags were used to study fresh weight evolution (EPF; daily fresh weight loss (PPFD and average fresh weight during the post-harvest period (PPFP. Italian breeds yielded above 9 t/ha, twice the Argentinean national average. According to the production results, Giove stood out regarding PFT, PFC, NTT, NTC and a proper DC, where the 70% of spears measured L and XL. With regard to postharvest behavior, it was found that the lowest degree of dehydration was obtained by using bags and trays.

  10. Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production

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    Ju Hee Lee

    2015-01-01

    Full Text Available The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549 and the major constituent, methyl protodioscin (MP, also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF- α from A549 cells at 10–100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS- induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100–400 mg/kg and 30–60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.

  11. Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.

    Science.gov (United States)

    Guida, Vincenzo; Cantarella, Maria; Chambery, Angela; Mezzacapo, Maria C; Parente, Augusto; Landi, Nicola; Severino, Valeria; Di Maro, Antimo

    2014-08-01

    Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies. The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively. N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max. In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively. Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content. We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.

  12. Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production.

    Science.gov (United States)

    Lee, Ju Hee; Lim, Hun Jai; Lee, Chan Woo; Son, Kun-Ho; Son, Jong-Keun; Lee, Sang Kook; Kim, Hyun Pyo

    2015-01-01

    The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10-100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100-400 mg/kg and 30-60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders. PMID:26379748

  13. 低温对芦笋萌芽及其激素含量的影响%Effect of Low Temperature on Bud Break and Changes of Endogenous Hormones of Asparagus

    Institute of Scientific and Technical Information of China (English)

    苗金龙; 乜兰春; 石立哲; 高飞; 贾丽丽

    2011-01-01

    以芦笋'Applo'为试材,在河北保定研究了低温对其萌芽及内源激素含量变化的影响.结果表明:自然条件下,芦笋在10月30日保温,仍可迅速萌芽生长;但11月14日和11月29日保温,萌芽数和采笋数显著减少;而在12月14日或之后保温,萌芽数与10月30日保温无显著差异,采笋数显著多于10月30日保温.说明芦笋在低温作用下一旦进入休眠阶段,需要满足一定的低温积累量,才能在保温后恢复正常生长.不同低温处理表明,2℃30 d或5℃45 d可满足'Applo'对低温的需求.芦笋在经历自然低温和低温处理过程中,鳞芽中GA3、IAA、ZR含量相对稳定,ABA含量先上升后又迅速下降,ABA含量变化与与芦笋休眠及休眠解除有密切关系.%Three-year-old plants of ‘Applo' were used to study the effect of low temperature on bud break and changes of endogenous hormones in bud of asparagus (Asparagus officinalis Linn) . The results showed that asparagus plant showed rapid bud break and spear growth when it was incubated on Oct. 30.But when it was incubated on Nov. 14 or 29, bud break and spear harvest decreased. While when it was incubated on Dec. 14 or later, the bud break had no difference with that incubated on Oct. 30, but spear harvest increased. These results suggested that once asparagus plants underwent the period of dormancy induced by the chilling in winter, they needed sufficient chilling duration to restore the activity of bud break and spear growth. The experiment of different low temperature treatment revealed that 2 ℃ 30 d or 5 ℃ 45 d could sastify the chilling duration of‘Applo'. During natural or treated chilling period, the content of GA3, IAA and ZR in bud of asparagus was relatively stable, but the content of ABA increased during dormancy stage, and then decreased rapidly to a lower level. The changes of ABA content had close relationship with dormancy and dormancy-breaking of asparagus.

  14. Composition Analysis and Nutritional Evaluation of the Asparagus%芦笋营养成分的分析评价

    Institute of Scientific and Technical Information of China (English)

    李翠霞; 毛箬青; 李志忠; 王永刚; 刘晓风

    2011-01-01

    本试验测定了97-2芦笋的营养成分,并对其营养价值与其他几个品系以及日常蔬菜进行了比较.结果表明,97-2芦笋含有较高的营养成分,每100 g该芦笋所含蛋白质、脂肪、总糖分别为3.25 g、0.25 g、2.64g,检测到了18种氨基酸,其中天冬氨酸115.13mg,占总氨基酸的3.05%,必需氨基酸占总氨基酸的34.95%.该芦笋的必需氨基酸评分和化学评分表明其第一限制性氨基酸为蛋氨酸+胱氨酸,必需氨基酸指数为41.13.该品系维生素含量丰富,其中VB1 0.39 mg、VB2 0.68 mg、VB3 0.38 mg、VB6 4.88 mg.此外还含有丰富的矿物质元素,其中Fe、Mg、Zn、Ca等元素含量较高于蔬菜.比较结果表明此芦笋含有较高的营养成分.此芦笋具有较高的营养价值,因此具有较好的开发前景.%The composition and nutrition of the hero asparagus new strain 97-2 asparagus was analyzed and compared with those of common vegetables. Contents of protein, fat and sugar in each 100 g of this asparagus were 3.25 g, 0.25 g and2.64 g, respectively. Eighteen amino acids in 97-2 asparagus were found, in which aspartic acid content was 115.13 mg, being 3.05% of total amino acids. The ratios of total essential amino acids to total amino acids (EAA/TAA) were 34.95%. According to the nutritional evaluation on amino acid score (AAS) and chemical score (CS), the first limited amino acids were methionine and cystine. The asparagus was rich of vitamin, containing VB1 0.391 mg, VB2 0.679 mg, VB3 0.380 mg, and VB6 4.880 mg. It was also rich in minerals, including Fe, Mg, Zn, Ca and other elements, which were high than those in vegetables. These results indicate that 97-2 asparagus possessed high nutritional value and health care function and thus has promising prospects for further development.

  15. 芦笋皮微晶纤维素的制备%Preparation of microcrystalline cellulose from asparagus peel

    Institute of Scientific and Technical Information of China (English)

    宋美玲

    2012-01-01

    以芦笋皮为原料,酶法制得膳食纤维后,采用盐酸水解,以溶胀性为指标确定制备微晶纤维素的工艺条件,为芦笋皮的加工利用提供一个新的途径。试验结果表明:在75℃下,盐酸浓度4 mol/L,料酸比1g∶5 mL,酸解时间5 h,制备出的微晶纤维素溶胀性可达6.90 mL/g,比芦笋皮中膳食纤维的溶胀性(4.80 mL/g)高2.10 mL/g。%Using asparagus as raw materials,microcrystalline cellulose was prepared in the best experimental conditions,determined by hydrochloric acid in different acidolysis time,acid concentration and proportion of material to acid,which took swelling as an index.And the best experimental conditions for preparation of microcrystalline cellulose provides a new way to process the asparagus.The results show that the best preparation conditions were the concentration of hydrochloric acid 4 mol/L,the proportion of material to acid 1∶5,acidolysis time 5 h,at 75 ℃.In the above conditions,the swelling of microcrystalline cellulose can be up to 6.90 mL/g,which is 2.10 mL/g more than the swelling(4.80 mL/g)of dietary fiber from the asparagus peel.

  16. Efficiency of applying insecticides to Isocerus sibirica larvae at the base of asparagus stems%芦笋茎基部施药对芦笋木蠹蛾幼虫的防治试验

    Institute of Scientific and Technical Information of China (English)

    张慧杰; 段国琪; 张战备; 张国强; 马利平; 王娇娟; 史高川

    2010-01-01

    芦笋木蠹蛾Isoceras sibirica(AlpIleraky)为芦笋(Asparagus officinalis(L.))上的一种蛀茎性害虫.试验结果表明,采用50%辛硫磷乳油300倍液在芦笋茎基部给药(5月中旬至6月中旬)为该虫的最佳化学防治方法,相对防治效果为92.5%,在所有处理中其加权Borda值最高(2.9).调整芦笋播期,可使定植当年最易受害的笋苗免于虫害.

  17. Asparagus stem as a new lignocellulosic biomass feedstock for anaerobic digestion: increasing hydrolysis rate, methane production and biodegradability by alkaline pretreatment.

    Science.gov (United States)

    Chen, Xiaohua; Gu, Yu; Zhou, Xuefei; Zhang, Yalei

    2014-07-01

    Recently, anaerobic digestion of lignocellulosic biomass for methane production has attracted considerable attention. However, there is little information regarding methane production from asparagus stem, a typical lignocellulosic biomass, by anaerobic digestion. In this study, alkaline pretreatment of asparagus stem was investigated for its ability to increase hydrolysis rate and methane production and to improve biodegradability (BD). The hydrolysis rate increased with increasing NaOH dose, due to higher removal rates of lignin and hemicelluloses. However, the optimal NaOH dose was 6% (w/w) according to the specific methane production (SMP). Under this condition, the SMP and the technical digestion time of the NaOH-treated asparagus stem were 242.3 mL/g VS and 18 days, which were 38.4% higher and 51.4% shorter than those of the untreated sample, respectively. The BD was improved from 40.1% to 55.4%. These results indicate that alkaline pretreatment could be an efficient method for increasing methane production from asparagus stem.

  18. Genetic resources collections of leafy vegetables (lettuce, spinach, chicory, artichoke, asparagus, lamb’s lettuce, rhubarb and rocket salad): composition and gaps

    NARCIS (Netherlands)

    Treuren, van R.; Coquin, P.; Lohwasser, U.

    2012-01-01

    Lettuce, spinach and chicory are generally considered the main leafy vegetables, while a fourth group denoted by ‘minor leafy vegetables’ includes, amongst others, rocket salad, lamb’s lettuce, asparagus, artichoke and rhubarb. Except in the case of lettuce, central crop databases of leafy vegetable

  19. Asparagus racemosus ameliorates cisplatin induced toxicities and augments its antileishmanial activity by immunomodulation in vivo.

    Science.gov (United States)

    Sachdeva, Heena; Sehgal, Rakesh; Kaur, Sukhbir

    2014-02-01

    Current drugs for the treatment of visceral leishmaniasis are inadequate and their efficacies are also compromised due to suppression of immune function associated during the course of infection. To overcome this problem, efforts are needed to develop therapies with effective immunomodulatory agents where decrease of parasitic burden and simultaneous enhancement of adaptive immunity can be achieved. In this study we have evaluated a new therapeutic approach based on combination of Asparagus racemosus, an immunomodulatory drug, in combination with cisplatin against Leishmania donovani infected BALB/c mice. We demonstrate that A. racemosus (650 mg/kg b.wt./day for 15 days, orally) in combination with cisplatin (5 mg/kg b.wt./day for 5 days, intraperitoneally) enhanced the clearance of parasites as determined by Giemsa-stained liver impression smears. Besides having better killing activity, this combination group achieved increased production of disease resolving Th-1 response (IFN-gamma, IL-2), heightened DTH (delayed type hypersensitivity) response and augmented levels of IgG2a. Moreover, A. racemosus in combination with cisplatin not only provided enhanced protective immune response but also resulted in remarkable improved kidney and liver function tests as manifested by normal levels of SGOT, SGPT, alkaline phosphatase, creatinine and urea in blood plasma with normal histological observations as compared to only cisplatin treated L. donovani infected BALB/c mice. Through this study we have ascertained that A. racemosus in combination with cisplatin in L. donovani infected BALB/c mice boosted as well as restored both cellular and humoral immunity. Thus in view of severe immunosuppression in visceral leishmaniasis, a better and effective strategy for optimum efficacy of future antileishmanial drugs would direct not only killing of parasite by the drug, but also simultaneous generation of immunity against the disease.

  20. Asparagus racemosus attenuates anxiety-like behavior in experimental animal models.

    Science.gov (United States)

    Garabadu, Debapriya; Krishnamurthy, Sairam

    2014-05-01

    Asparagus racemosus Linn. (AR) is used worldwide as a medicinal plant. In the present study, the anxiolytic activity of standardized methanolic extract of root of AR (MAR) was evaluated in open-field test (OFT), hole-board, and elevated plus maze (EPM) tests. Rats received oral pretreatment of MAR in the doses of 50, 100, and 200 mg/kg daily for 7 days and then were evaluated for the anxiolytic activity in different animal models. Both MAR (100 and 200 mg/kg) and diazepam (1 mg/kg, p.o.) increased the grooming behavior, number of central squares crossed, and time spent in the central area during OFT. Further, MAR (100 and 200 mg/kg) increased the head-dip and head-dip/sniffing behavior, and decreased sniffing activity in hole-board test. Furthermore, MAR (100 and 200 mg/kg) increased the percentage entries and time spent to open arm in EPM test paradigm. The anxiolytic activity in the experimental models was similar to that of diazepam. MAR (100 and 200 mg/kg) enhanced the level of amygdalar serotonin and norepinephrine. It also increased the expression of 5-HT2A receptors in the amygdala. In another set of experiment, flumazenil attenuated the anxiolytic effect of minimum effective dose of MAR (100 mg/kg) in OFT, hole-board, and EPM tests, indicating GABAA-mediated mechanism. Moreover, the anxiolytic dose of MAR did not show sedative-like effect in OFT and EPM tests compared to diazepam (6 mg/kg, p.o.). Thus, the anxiolytic response of MAR may involve GABA and serotonergic mechanisms. These preclinical data show that AR can be a potential agent for treatment of anxiety disorders.

  1. Asparagus Root Regulates Cholesterol Metabolism and Improves Antioxidant Status in Hypercholesteremic Rats

    Directory of Open Access Journals (Sweden)

    Nishant P. Visavadiya

    2009-01-01

    Full Text Available Hyperlipidemia/hypercholesteremia are major risk factors for atherosclerosis and cardiovascular diseases. Root of Asparagus racemosus (AR is widely used in Ayurvedic system of medicine in India and is known for its steroidal saponin content. This study was designed to investigate the hypocholesteremic and antioxidant potential of AR root in both normo- and hypercholesteremic animals. Normal and hypercholesteremic male albino rats were administered with root powder of AR (5 and 10 g% dose levels along with normal and hypercholesteremic diets, respectively, for a duration of 4 weeks. Plasma and hepatic lipid profiles, fecal sterol, bile acid excretion and hepatic antioxidant activity were assessed. Inclusion of AR root powder in diet, resulted in a dose-dependant reduction in plasma and hepatic lipid profiles, increased fecal excretion of cholesterol, neutral sterol and bile acid along with increases in hepatic HMG-CoA reductase activity and bile acid content in hypercholesteremic rats. Further, AR root also improved the hepatic antioxidant status (catalase, SOD and ascorbic acid levels. No significant changes in lipid and antioxidant profiles occurred in the normocholesteremic rats administered with AR root powder. AR root appeared to be useful as a dietary supplement that offers a protection against hyperlipidemia/hypercholesteremia in hypercholesteremic animals. The results of the present study indicate that the potent therapeutic phyto-components present in AR root i.e. phytosterols, saponins, polyphenols, flavonoids and ascorbic acid, could be responsible for increased bile acid production, elimination of excess cholesterol and elevation of hepatic antioxidant status in hypercholesteremic conditions.

  2. Anti-inflammatory activity of liposomes of Asparagus racemosus root extracts prepared by various methods

    Science.gov (United States)

    Plangsombat, Nathsiree; Rungsardthong, Kanin; Kongkaneramit, Lalana; Waranuch, Neti; Sarisuta, Narong

    2016-01-01

    Asparagus racemosus root extracts (AR) have been reported to possess a variety of pharmacological properties. The aim of the present study was to develop liposomes of AR and to assess their physicochemical characteristics and anti-inflammatory activity in the monocytic leukemia cell line THP-1. Liposomes containing various ratios of AR to lipid and a phosphatidylcholine to cholesterol molar ratio of 7:3 were prepared by thin-film hydration (TF), reverse-phase evaporation (REV) and polyol dilution (PD). The results showed that AR liposomes prepared by TF had a multilamellar structure and a large size, whereas those prepared by REV and PD were oligolamellar in structure, and of a smaller size. The particle sizes and zeta potentials of the liposomes ranged from 196.5 to 456.6 nm and from −4.34 to −18.94 mV, respectively. The AR to lipid ratio was shown to have no significant influence on particle size, while the zeta potential generally increased with increasing AR to lipid ratio. The highest entrapment efficiency values were detected in liposomes with an AR to lipid ratio of 1:5, and for liposomes prepared by TF, REV and PD methods, the entrapment efficiencies were 55.71±2.04, 56.21±3.59 and 67.68±1.37%, respectively. AR was found to exert no toxicity on THP-1 cells. The maximum anti-inflammatory activities of AR and AR liposomes, evaluated in terms of the percentage inhibition of tumor necrosis factor-α in THP-1 cells, were ~52% at a concentration of 1 µg/ml. It can be concluded from the present study that AR liposomes have the potential to be used a formulation for topical and/or transdermal drug delivery to provide anti-inflammatory activity. PMID:27698785

  3. Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation.

    Science.gov (United States)

    Sung, Ji-Eun; Lee, Hyun-Ah; Kim, Ji-Eun; Go, Jun; Seo, Eun-Ji; Yun, Woo-Bin; Kim, Dong-Seob; Son, Hong-Joo; Lee, Chung-Yeoul; Lee, Hee-Seob; Hwang, Dae-Youn

    2016-03-01

    Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin inflammation. However, few studies have investigated its effects and IL-4 correlation on skin inflammation to date. To quantitatively evaluate the suppressive effects of ethyl acetate extracts of A. cochinchinensis (EaEAC) on phthalic anhydride (PA)-induced skin inflammation and investigate the role of IL-4 during their action mechanism, alterations in general phenotype biomarkers and luciferase-derived signals were measured in IL-4/Luc/CNS-1 transgenic (Tg) mice with PA-induced skin inflammation after treatment with EaEAC for 2 weeks. Key phenotype markers including lymph node weight, immunoglobulin E (IgE) concentration, epidermis thickness and number of infiltrated mast cells were significantly decreased in the PA+EaEAC treated group compared with the PA+Vehicle treated group. In addition, expression of IL-1β and TNF-α was also decreased in the PA+EaEAC cotreated group, compared to PA+Vehicle treated group. Furthermore, a significant decrease in the luciferase signal derived from IL-4 promoter was detected in the abdominal region, submandibular lymph node and mesenteric lymph node of the PA+EaEAC treated group, compared to PA+Vehicle treated group. Taken together, these results suggest that EaEAC treatment could successfully improve PA-induced skin inflammation of IL-4/Luc/CNS-1 Tg mice, and that IL-4 cytokine plays a key role in the therapeutic process of EaEAC. PMID:27051441

  4. Effect of plastic mulching on mycotoxin occurrence and mycobiome abundance in soil samples from asparagus crops.

    Science.gov (United States)

    Muñoz, K; Schmidt-Heydt, M; Stoll, D; Diehl, D; Ziegler, J; Geisen, R; Schaumann, G E

    2015-11-01

    Plastic mulching (PM) is widely used in modern agriculture because of its advantageous effects on soil temperature and water conservation, factors which strongly influence the microbiology of the soil. The aim of this study was to assess the effect of PM on mycotoxin occurrence in relation with mycobiome abundance/diversity and soil physicochemical properties. Soil samples were collected from green (GA) and white asparagus (WA) crops, the last under PM. Both crops were cultivated in a ridge-furrow-ridge system without irrigation. Samples were analyzed for mycotoxin occurrence via liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Total colony-forming unit was indicative of mycobiome abundance, and analysis of mycobiome diversity was performed by internal transcribed spacer (ITS) sequencing. PM avoided the drop of soil temperature in winter and allowed higher soil temperature in early spring compared to non-covered soil. Moreover, the use of PM provided controlled conditions for water content in soil. This was enough to generate a dissimilar mycotoxin occurrence and mycobiome diversity/abundance in covered and non-covered soil. Mycotoxin soil contamination was confirmed for deoxynivalenol (DON), range LOD to 32.1 ng/g (LOD = 1.1 ng/g). The DON values were higher under PM (average 16.9 ± 10.1 ng/g) than in non-covered soil (9.1 ± 7.9 ng/g); however, this difference was not statically significant (p = 0.09). Mycobiome analysis showed a fungal compartment up to fivefold higher in soil under PM compared to GA. The diversity of the mycobiome varied between crops and also along the soil column, with an important dominance of Fusarium species at the root zone in covered soils. PMID:26412448

  5. Effect of Enzyme-Treated Asparagus Extract (ETAS) on Psychological Stress in Healthy Individuals.

    Science.gov (United States)

    Takanari, Jun; Nakahigashi, Jun; Sato, Atsuya; Waki, Hideaki; Miyazaki, Shogo; Uebaba, Kazuo; Hisajima, Tatsuya

    2016-01-01

    The aim of this study was to examine the effectiveness of Enzyme-Treated Asparagus Extract (ETAS) on improving stress response. A randomized, double-blind, placebo-controlled cross-over trial was undertaken in healthy volunteers. ETAS (150 mg/d) or a placebo was consumed for 28 d, with a washout period. Psychological parameters were examined using a self-report scale questionnaire and psychological stress was applied using the Uchida-Kraepelin (U-K) test. During the stress load, autonomic nervous function was analyzed. After the stress load, a profile of mood states (POMS) psychological rating was performed, and serum cortisol, plasma catecholamine, salivary secretory immunoglobulin A (sIgA), and salivary cortisol were analyzed. ETAS intake improved the self-reported rating for the items "Feel tired," "Hard to get up," and "Feel heavy" in the psychological questionnaire; ameliorated the self-reported rating for the items "Depression-Dejection" and "Fatigue" in the POMS questionnaire; and increased salivary sIgA levels after the U-K test. In contrast, serum and salivary cortisol levels, and plasma catecholamine did not change. During the U-K test, ETAS significantly upregulated the sympathetic nerve activity. Furthermore, ETAS intake significantly increased the number of answers and the number of correct answers in the U-K test, suggesting that it might improve office work performance with swiftness and accuracy under stressful conditions. In conclusion, ETAS supplementation reduced feelings of dysphoria and fatigue, ameliorated quality of sleep, and enhanced stress-load performance as well as promoted stress response by increasing salivary sIgA levels. These data suggest ETAS intake may exert beneficial effects, resulting from well-controlled stress management, in healthy individuals. PMID:27465727

  6. Application of Feature Space in Extraction of Asparagus Planting Area Using Renote Sensing%芦笋种植面积遥感提取

    Institute of Scientific and Technical Information of China (English)

    王猛; 隋学艳; 梁守真; 姚慧敏; 侯学会

    2016-01-01

    The traditional method of extracted cash crop planting area by remote sensing has been widely carried out. However,it is not suitable for the planting area extraction of asparagus.Aiming the deficiency of the existing methods,this paper considered the characteristics of asparagus production.Taking Caoxian county as the study area,the paper studied the method of extraction of asparagus planting area by using Landsat 8 images.By comparing the different NDVI between the asparagus planting area and other objects,this paper first removed the water and wheat field by NDVI threshold segmentation method,and found the distribution of soil line by further analyzing the two-dimensional feature space of the asparagus planting area,buildings and roads.Finally,the asparagus planting area was extracted through the defined thresholds.The results showed that the asparagus planting area is 14626.55ha2 acres in Caoxian county,where the accuracy achieves 84.85%.%针对传统遥感技术提取芦笋种植面积精度不高的问题,根据芦笋的种植特点,该文以山东省曹县为研究区域,以 Landsat 8影像为研究数据,提出了芦笋种植面积的提取方法。通过分析芦笋种植区与其他地物归一化差值植被指数特征,首先利用阈值分割方法去除水体、小麦地物,进一步分析芦笋种植区、建筑物和道路等的影像二维特征空间,发现芦笋种植区的土壤线分布规律,并通过波段运算结果确定芦笋种植区阈值,最后进行芦笋种植面积提取。结果表明,曹县的芦笋种植面积为14626.55ha2,总体精度为84.85%。

  7. Chemical analysis reveals the botanical origin of shatavari products and confirms the absence of alkaloid asparagamine A in Asparagus racemosus.

    Science.gov (United States)

    Kumeta, Yukie; Maruyama, Takuro; Wakana, Daigo; Kamakura, Hiroyuki; Goda, Yukihiro

    2013-01-01

    Shatavari-a famous Ayurveda materia medica used mainly as a tonic for women-is distributed in health food products all over the world. The Ayurvedic Pharmacopoeia of India identifies the botanical origin of shatavari as the tuberous root of Asparagus racemosus. We recently investigated by DNA analysis the botanical origin of shatavari products on the Japanese market. The results suggested that their botanical origin was Asparagus; however, species identification was difficult. In this study, we analyzed steroidal saponins, including those specific to this plant, in these products and confirmed their origin as A. racemosus. Next, alkaloid analyses of an authentic A. racemosus plant and these products were performed, because several papers have reported the isolation of a pyrrolo[1,2-a]azepine alkaloid, asparagamine A, from this plant. Our results suggest that neither plant material nor products contained asparagamine A. It has been pointed out that Stemona plants are sometimes mistaken for shatavari, because their tuberous roots have a similar shape to that of A. racemosus, and pyrrolo[1,2-a]azepine alkaloids are thought to be Stemona-specific. These data strongly suggest that A. racemosus does not contain asparagamine A, and that previous isolation of asparagamine A from materials claimed as originating from A. racemosus was likely caused by misidentification of Stemona plants as A. racemosus.

  8. Effects of enzyme-treated asparagus extract on heat shock protein 70, stress indices, and sleep in healthy adult men.

    Science.gov (United States)

    Ito, Tomohiro; Goto, Kazunori; Takanari, Jun; Miura, Takehito; Wakame, Koji; Nishioka, Hiroshi; Tanaka, Aiko; Nishihira, Jun

    2014-01-01

    Enzyme-treated asparagus extract (ETAS) has been developed as a novel anti-stress functional food ingredient that is produced from asparagus. Two human intervention trials with ETAS were conducted in healthy adult male volunteers. Study 1 was a randomized, double-blind, placebo-controlled study to assess the effects of ETAS on expression of heat shock protein 70 (HSP70) mRNA in blood and the autonomic nervous system (ANS). The ETAS group showed a tendency to enhance HSP70 mRNA expression level compared to the placebo group. Several ANS condition parameters were significantly improved in the ETAS group when compared to the placebo group. In Study 2, a randomized, double-blind, placebo-controlled, crossover trial investigated the influence on stress-related hormones and sleep. Serum and salivary cortisol levels were significantly elevated compared to baseline during the placebo period, but remained unchanged during the ETAS period. The salivary chromogranin A level was significantly decreased in the ETAS-treated subjects compared to their baseline levels. The actual sleep time was not significantly different between ETAS and placebo. However, when the subjects were divided into two categories based on sleep efficiency or the average of night sleeping time, ETAS intake was effective to modulate the sleep state among those with low sleep efficiency or excess sleep time.

  9. Effects of enzyme-treated asparagus extract on heat shock protein 70, stress indices, and sleep in healthy adult men.

    Science.gov (United States)

    Ito, Tomohiro; Goto, Kazunori; Takanari, Jun; Miura, Takehito; Wakame, Koji; Nishioka, Hiroshi; Tanaka, Aiko; Nishihira, Jun

    2014-01-01

    Enzyme-treated asparagus extract (ETAS) has been developed as a novel anti-stress functional food ingredient that is produced from asparagus. Two human intervention trials with ETAS were conducted in healthy adult male volunteers. Study 1 was a randomized, double-blind, placebo-controlled study to assess the effects of ETAS on expression of heat shock protein 70 (HSP70) mRNA in blood and the autonomic nervous system (ANS). The ETAS group showed a tendency to enhance HSP70 mRNA expression level compared to the placebo group. Several ANS condition parameters were significantly improved in the ETAS group when compared to the placebo group. In Study 2, a randomized, double-blind, placebo-controlled, crossover trial investigated the influence on stress-related hormones and sleep. Serum and salivary cortisol levels were significantly elevated compared to baseline during the placebo period, but remained unchanged during the ETAS period. The salivary chromogranin A level was significantly decreased in the ETAS-treated subjects compared to their baseline levels. The actual sleep time was not significantly different between ETAS and placebo. However, when the subjects were divided into two categories based on sleep efficiency or the average of night sleeping time, ETAS intake was effective to modulate the sleep state among those with low sleep efficiency or excess sleep time. PMID:25297618

  10. Development of a rapid HPLC-UV method for simultaneous quantification of protodioscin and rutin in white and green asparagus spears.

    Science.gov (United States)

    Lee, Eun Jin; Yoo, Kil Sun; Patil, Bhimanagouda S

    2010-01-01

    Asparagus (Asparagus officinalis L.) spears are rich in bioactive compounds such as protodioscin, a saponin, and rutin, a flavonoid. Protodioscin and rutin are routinely quantified separately, and an approach permitting simultaneous measurement would significantly improve speed of analysis. We have optimized an extraction procedure and modified a method of high-performance liquid chromatography by coupling to an ultraviolet detector to simultaneously analyze protodioscin and rutin in asparagus extracts. An acidic ethanol solvent was more efficient than methanol, acetonitrile, or water in coextraction of protodioscin and rutin. Protodioscin and rutin were detected at 210 nm, with retention times of 12.6 min and 7.9 min, respectively. The method was validated by high linear correlations between 3.13 and 1000.0 μg/mL for protodioscin (r(2)= 0.9999), and between 0.3 and 1087.5 μg/mL for rutin (r(2)= 0.9997). The limit(s) of detection and quantification for protodioscin were 1.6 μg/mL and 3.13 μg/mL, respectively, and for rutin 0.2 μg/mL and 0.3 μg/mL, respectively. White asparagus spears and the crown of the plants were revealed to be rich sources of protodioscin and contained 2.59 to 10.4 mg/g dry weight. Green asparagus spears, particularly the upper portion, were rich in rutin and contained between 1.51 and 7.29 mg/g dry weight.

  11. 速溶芦笋粉对小鼠免疫功能的影响%Effects of Instant Asparagus Powder on Immune Function of Mice

    Institute of Scientific and Technical Information of China (English)

    李汉臣; 赵希艳; 刘绍军; 彭友顺

    2012-01-01

    [ Objective] To study the effects of instant asparagus powder on immune function of mice. [ Method] According to the method recorded in the Technical Standards for Testing & Assessment of Health Food (2003), the mice were given instant asparagus powder (referred to as the extract of Asparagus offwinalis) for successive 30 days by intragastric administration. [ Result ] Instant asparagus powder could improve evidently funtions of cellular immunity, humoral immunity, mononuclear macrophage and activity of NK on mice. But apparent effect to weights of spleen, thynuis and body were not seen in the trials. [ Conclusion] Instant asparagus powder can improve the immune function of mice.%[目的]研究速溶芦笋粉对小鼠免疫功能的影响.[方法]根据《保健食品检验与评价技术规范》(2003年版)的方法,将速溶芦笋粉(芦笋提取物)对小鼠进行灌胃给药,持续30 d,检验其对小鼠免疫能力的影响.[结果]速溶芦笋粉能明显增强小鼠细胞免疫和体液免疫能力,也能明显增强小鼠单核-巨噬细胞功能和NK细胞活性,但对各剂量组小鼠体重及脾脏和胸腺的脏器指数无明显影响.[结论]速溶芦笋粉具有增强免疫力的功能.

  12. Effect of Chemical Fertilization on Yield and Quality of Green Asparagus%化肥施用对绿芦笋产量和品质的影响

    Institute of Scientific and Technical Information of China (English)

    翟海翔; 杨斌; 焦彩菊

    2011-01-01

    A field experience was conducted to study the effect of NPK fertilization on yield and quality of green asparagus. The result showed that the branches of green asparagus, weight of per branch and yield of green asparagus in NPK treatment had the maximum values among those five treatments. The branches of green asparagus and weight of per branches in treatment of PK, NK and NP were much lower than NPK at different level. The content of crude protein, soluble protein, soluble sugar and content of mineral element in NPK treatment were much higher than in the other treatments. We concluded that balanced nutrient applications could not only increased yield, but also improved quality of green asparagus.%在大田条件下,研究了不同氮磷钾肥对绿芦笋产量及品质的影响.结果表明,绿芦笋支数、单支质量以及总产量均以NPK处理最高;PK,NK以及NP处理的芦笋支数、单支质量均不同程度降低,最终导致减产.芦笋粗蛋白、粗脂肪、粗纤维、可溶性蛋白、可溶性糖以及不同矿质元素含量均以NPK处理最高.可见,平衡的养分供给不仅可以提高绿芦笋产量,而且还可以有效改善芦笋的品质.

  13. Characterization of Fusarium isolates from asparagus fields in southwestern Ontario and influence of soil organic amendments on Fusarium crown and root rot.

    Science.gov (United States)

    Borrego-Benjumea, Ana; Basallote-Ureba, María J; Melero-Vara, José M; Abbasi, Pervaiz A

    2014-04-01

    Fusarium crown and root rot (FCRR) of asparagus has a complex etiology with several soilborne Fusarium spp. as causal agents. Ninety-three Fusarium isolates, obtained from plant and soil samples collected from commercial asparagus fields in southwestern Ontario with a history of FCRR, were identified as Fusarium oxysporum (65.5%), F. proliferatum (18.3%), F. solani (6.4%), F. acuminatum (6.4%), and F. redolens (3.2%) based on morphological or cultural characteristics and polymerase chain reaction (PCR) analysis with species-specific primers. The intersimple-sequence repeat PCR analysis of the field isolates revealed considerable variability among the isolates belonging to different Fusarium spp. In the in vitro pathogenicity screening tests, 50% of the field isolates were pathogenic to asparagus, and 22% of the isolates caused the most severe symptoms on asparagus. The management of FCRR with soil organic amendments of pelleted poultry manure (PPM), olive residue compost, and fish emulsion was evaluated in a greenhouse using three asparagus cultivars of different susceptibility in soils infested with two of the pathogenic isolates (F. oxysporum Fo-1.5 and F. solani Fs-1.12). Lower FCRR symptom severity and higher plant weights were observed for most treatments on 'Jersey Giant' and 'Grande' but not on 'Mary Washington'. On all three cultivars, 1% PPM consistently reduced FCRR severity by 42 to 96% and increased plant weights by 77 to 152% compared with the Fusarium control treatment. Populations of Fusarium and total bacteria were enumerated after 1, 3, 7, and 14 days of soil amendment. In amended soils, the population of Fusarium spp. gradually decreased while the population of total culturable bacteria increased. These results indicate that soil organic amendments, especially PPM, can decrease disease severity and promote plant growth, possibly by decreasing pathogen population and enhancing bacterial activity in the soil.

  14. Development of a rapid HPLC-UV method for simultaneous quantification of protodioscin and rutin in white and green asparagus spears.

    Science.gov (United States)

    Lee, Eun Jin; Yoo, Kil Sun; Patil, Bhimanagouda S

    2010-01-01

    Asparagus (Asparagus officinalis L.) spears are rich in bioactive compounds such as protodioscin, a saponin, and rutin, a flavonoid. Protodioscin and rutin are routinely quantified separately, and an approach permitting simultaneous measurement would significantly improve speed of analysis. We have optimized an extraction procedure and modified a method of high-performance liquid chromatography by coupling to an ultraviolet detector to simultaneously analyze protodioscin and rutin in asparagus extracts. An acidic ethanol solvent was more efficient than methanol, acetonitrile, or water in coextraction of protodioscin and rutin. Protodioscin and rutin were detected at 210 nm, with retention times of 12.6 min and 7.9 min, respectively. The method was validated by high linear correlations between 3.13 and 1000.0 μg/mL for protodioscin (r(2)= 0.9999), and between 0.3 and 1087.5 μg/mL for rutin (r(2)= 0.9997). The limit(s) of detection and quantification for protodioscin were 1.6 μg/mL and 3.13 μg/mL, respectively, and for rutin 0.2 μg/mL and 0.3 μg/mL, respectively. White asparagus spears and the crown of the plants were revealed to be rich sources of protodioscin and contained 2.59 to 10.4 mg/g dry weight. Green asparagus spears, particularly the upper portion, were rich in rutin and contained between 1.51 and 7.29 mg/g dry weight. PMID:21535581

  15. Study on the sleep improvement effect of the instant asparagus powder%速溶芦笋粉改善睡眠功能的研究

    Institute of Scientific and Technical Information of China (English)

    马淑凤; 王芳; 李汉臣; 常学东; 王周平; 黄云祥

    2012-01-01

    Objective:To study the sleep improvement effect of instant asparagus powder on mice.Methods:Healthy KM male mice with the body weight of 18~22g were divided into groups randomly,then mice were gavaged with the different dosage of instant asparagus powder and jujubosided respectively everyday,and the direct sleep effect of the test material on mice were observed.30d later,the hypnosis test under subthreshold dose of Sodium Pentobarbital,the incubation period test with Barbital Sodium and the sleep extending test with Sodium Pentobarbital were conducted.Results:The sleep rate of low,medium and high dose group of instant asparagus powder were 25.00%,33.33% and 41.67% respectively,significantly raised the sleep rate comparing with the control group,it showed that the instant asparagus powder had hypnotic effect under the threshold hypnotic dosage of pentobarbital sodium.The sleeping incubation period of low,medium and high dose group of instant asparagus powder were 23.09,20.29,20.22min respectively,compared with the control group time 32.36min,instant asparagus powder significantly shorten the sleep incubation period of mice.The sleep time of low,medium and high dose group of instant asparagus powder were 35.89,40.62,45.78min respectively,the result showed that instant asparagus powder could significantly prolong sleep time that induced by pentobarbital sodium.Conclusion:Instant asparagus powder could improve the sleeping quality of mice.%目的:研究速溶芦笋粉对小鼠改善睡眠的影响。方法:18~22g健康雄性昆明种小鼠,按体重随机分组,每天经口灌胃不同剂量速溶芦笋粉和阳性对照物(酸枣仁皂苷),观察小鼠直接睡眠作用,连续给予受试物30d后,进行戊巴比妥钠阈下剂量催眠实验、巴比妥钠睡眠潜伏期实验、延长戊巴比妥钠睡眠时间实验。结果:速溶芦笋粉的低中、高剂量组的睡眠发生率分别为25.00%、33.33%和41.67%,均比空白对照组显著增

  16. 不同钾肥用量对绿芦笋产量及营养品质的影响%Effects of Different Amount of Potassium Fertilizer on Yield and Nutrient Quality of Green Asparagus

    Institute of Scientific and Technical Information of China (English)

    张瑞富; 杨恒山; 刘晶; 李国红; 王翠花

    2013-01-01

    In experimental farm of Inner Mongolia University for Nationalities from March to July in 2009, using 5-year Asparagus officinalis L.‘Apollo’as experimental material, the author studied the effects of different amount of potassium fertilizer on yield and nutrient quality of green asparagus. The results were as the following:the yield of green Asparagus and the weight of per branches of green asparagus was the first increase after decreasing trend with the increase of the amount of potassium fertilizer when the amount of potassium fertilizer was within 0-375 kg/hm2. And the yield and the weight of per branches was the highest when the amount of potassium fertilizer was respectively 300 kg/hm2 and 225 kg/hm2; the number of green Asparagus went up with the increase of the amount of potassium fertilizer. The content of soluble protein at each stage was gradually increased with the increase of the amount of potassium fertilizer. The content of soluble sugar in the early and middle increased first and then decreased of which the highest was the amount of potassium fertilizer of 225 kg/hm2 in the early, was the amount of potassium fertilizer of 330 kg/hm2 in the middle, gradually increased in the late. The content of crude protein went up with the increase of the amount of potassium fertilizer and other nutritional content had no obvious change. Ca, Mg elements gradually decreased with increasing potassium fertilizer and other mineral elements with no obvious relation of potassium fertilizer. Under this experimental condition, the combination of yield and nutrient quality, the amount of potassium fertilizer of 300 kg/hm2 was appropriate for green Asparagus.%2009年3-7月在西辽河平原的内蒙古民族大学实验农场,以5年生芦笋‘阿波罗’(Asparagus officinalisL.‘Apollo’)为试材,研究不同钾肥用量对绿芦笋产量及营养品质的影响。结果表明,施钾量在0~375 kg/hm2的范围内,随着钾肥用量的增加绿芦笋产量

  17. 光照对绿芦笋汁色泽稳定性的影响%Effect of Light Exposure on Color Stability of Green Asparagus Juice

    Institute of Scientific and Technical Information of China (English)

    陈学红; 秦卫东; 马利华; 郑永华; 郁步青

    2015-01-01

    To explore the effect of light exposure on color stability of green asparagus juice during storage time ,the pasteurized juice were exposed to natural light and dark at ambient temperature .The changes of color ,chlorophyll ,carotenoids ,vitamin C ,total phenolics ,reducing sugar and amino acids con‐tents we re studied in green asparagus juice during storage time .The results showed that light exposure sig‐nificantly affected the color stability of green asparagus juice .The decrease of chlorophyll and the increase of carotenoids contents were inhibited by dark storage .At the same time ,the greenness of asparagus juice was kept .Vitamin C ,total phenolics and reducing sugar contents were closely correlated with the color of green asparagus juice .There was no significant correlation between amino acids and color of green aspara‐gus juice .The decrease of vitamin C ,total phenolics and reducing sugar contents were prevented by dark storage and the color stability of green asparagus juice was effectively kept .%为了探讨光对绿芦笋汁色泽稳定性的影响,将杀菌处理后的绿芦笋汁于自然光及黑暗(避光)条件下常温贮藏,研究绿芦笋汁在贮藏期间的色泽、叶绿素、类胡萝卜素、维生素C、总酚、还原糖和氨基酸质量浓度的变化。结果表明:光照对绿芦笋汁的色泽稳定性影响显著;避光贮藏可有效延缓绿芦笋汁叶绿素的降解,抑制类胡萝卜素质量浓度的上升,保持绿芦笋汁的绿色;维生素C、总酚和还原糖与绿芦笋汁的色泽密切相关,氨基酸与其色泽无显著相关性,避光贮藏可有效抑制维生素C、总酚和还原糖质量浓度的降低,从而有效地维持了绿芦笋汁的色泽稳定性。

  18. Studies on Immune Regulation Effect of Ethanol Extract from Asparagus%芦笋醇提取物的免疫调节作用

    Institute of Scientific and Technical Information of China (English)

    王芳; 马淑凤; 李汉臣; 王周平; 黄云祥; 代卉

    2013-01-01

    In this study,air dried asparagus was ultrasonic extracted with 75 % (v/v)ethanol. The immune function of this extract on mice were investigated . Mice were orally administrated with asparagus extract at three dose levels (7,70,140 mg/Kg BW) for 30 days. At the organ level, thymus and spleen were weighed and their indices were calculated. The macrophage phagocytosis ability in vitro, transformation of spleen lyphmocytes, and serum hemolysin were investigated to assess the immune functions. The results showed that the middle and high dosage of asparagus extract promoted the spleen index and the phagocytic function of murine peritoneal macrophages. the middle and high dosage of asparagus extract significantly enhanced the transformation of spleen lyphmocytes and serum hemolysin (HC50). This suggest that asparagus extract can enhance both the specific and non-specific immune functions in normal mice. Moreover, the specific immune effects of asparagus extract especially depended on the dosage.%分析芦笋醇提取物对机体免疫功能的调节作用.将风干芦笋用75%体积分数的乙醇溶液进行超声提取,获得芦笋醇提取物.对实验小鼠灌胃芦笋醇提取物,剂量分别设为7、70、140 mg/kg,另设一个空白对照组灌胃等体积生理盐水,连续30 d灌胃后,分别进行小鼠脏器/体重、腹腔巨噬细胞吞噬实验、淋巴细胞转化实验及半数溶血值实验.结果显示,中、高剂量组的芦笋提取物可显著提高小鼠的脾脏指数和腹腔巨噬细胞的吞噬能力;中、高剂量组的芦笋提取物具有显著增强淋巴细胞增值能力的作用,对提高血清溶血素含量作用极显著,并具有剂量依赖性.表明芦笋提取物能增强正常小鼠的非特异性和特异性免疫功能.

  19. Produtividade do aspargo sob irrigação na região do Submédio São Francisco Asparagus yield under irrigation at the Submédio São Francisco region

    Directory of Open Access Journals (Sweden)

    Lúcio O. B. D'Oliveira

    1999-03-01

    Full Text Available O objetivo do presente trabalho foi avaliar uma coleção de cultivares e híbridos de aspargo (Asparagus officinalis L., quanto à produtividade e adaptabilidade às condições do Submédio São Francisco. A avaliação foi realizada no Campo Experimental de Bebedouro (Embrapa Semi-Árido, em Petrolina - PE, durante o período de 1991 a 1994. A coleção, constituída por duas cultivares e doze híbridos provenientes de Pelotas - RS, dez híbridos da França e três híbridos dos Estados Unidos, foi implantada em 15 de agosto de 1990. Na avaliação da produtividade, considerou-se apenas os turiões brancos, retos, sem manchas ou lesões, com diâmetro acima de 8 mm e comprimento variando de 15 a 20 cm. A produtividade média por genótipo durante o período de 1991 a 1994 variou de 1,74 a 6,33 t. ha-1. O ano de 1992 destacou-se com a maior produtividade média (4,46 t. ha-1. Os híbridos de origem francesa apresentaram uma acentuada mortalidade de plantas, indicando uma provável inadaptação às condições da região. Os híbridos W 12 x 14, G 4 x 14 e G 103 x 14, procedentes de Pelotas, destacaram-se com produtividades médias superiores a 4,40 t. ha-1. A cultivar New Jersey 220 apresentou o melhor desempenho, com 3,69 t. ha-1. Estes dados indicam o aspargo como uma boa alternativa agrícola para os perímetros irrigados da região Nordeste do Brasil, oferecendo, inclusive, condições de a região competir nos mercados interno e externo.The objectives of this study were to evaluate genotypes of asparagus (Asparagus officinalis L., in terms of yield and adaptation to conditions of the Submédio São Francisco region. The research was conducted between 1991 and 1994 at the Bebedouro Experimental Station of Embrapa Semi-Árido, in Petrolina, Pernambuco State. Two standard cultivars and twelve Brazilian hybrids (Pelotas and Rio Grande do Sul, ten French hybrids, and three hybrids from the United States were tested. Only straight spears

  20. ETAS, an enzyme-treated asparagus extract, attenuates amyloid beta-induced cellular disorder in PC12 cells.

    Science.gov (United States)

    Ogasawara, Junetsu; Ito, Tomohiro; Wakame, Koji; Kitadate, Kentaro; Sakurai, Takuya; Sato, Shogo; Ishibashi, Yoshinaga; Izawa, Tetsuya; Takahashi, Kazuto; Ishida, Hitoshi; Takabatake, Ichiro; Kizaki, Takako; Ohno, Hideki

    2014-04-01

    One of the pathological characterizations of Alzheimer's disease (AD) is the deposition of amyloid beta peptide (Abeta) in cerebral cortical cells. The deposition of Abeta in neuronal cells leads to an increase in the production of free radicals that are typified by reactive oxygen species (ROS), thereby inducing cell death. A growing body of evidence now suggests that several plant-derived food ingredients are capable of scavenging ROS in mammalian cells. The purpose of the present study was to investigate whether enzyme-treated asparagus extract (ETAS), which is rich in antioxidants, is one of these ingredients. The pre-incubation of differentiated PC 12 cells with ETAS significantly recovered Abeta-induced reduction of cell viability, which was accompanied by reduced levels of ROS. These results suggest that ETAS may be one of the functional food ingredients with anti-oxidative capacity to help prevent AD.

  1. Asparagus cochinchinensis Extract Alleviates Metal Ion-Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents

    Directory of Open Access Journals (Sweden)

    Weiyu Zhang

    2016-01-01

    Full Text Available Metal ions and sulfate are components of atmospheric pollutants that have diverse ways of entering the human body. We used Drosophila as a model to investigate the effect of Asparagus cochinchinensis (A. cochinchinensis extracts on the gut and characterized gut homeostasis following the ingestion of metal ions (copper, zinc, and aluminum. In this study, we found that the aqueous A. cochinchinensis extract increased the survival rate, decreased epithelial cell death, and attenuated metal ion-induced gut morphological changes in flies following chronic exposure to metal ions. In addition, we screened out, by network pharmacology, six natural products (NPs that could serve as putative active components of A. cochinchinensis that prevented gut injury. Altogether, the results of our study provide evidence that A. cochinchinensis might be an effective phytomedicine for the treatment of metal ion-induced gut injury.

  2. Effect of Asparagus racemosus (shatavari) extract on physicochemical and functional properties of milk and its interaction with milk proteins.

    Science.gov (United States)

    Veena, N; Arora, Sumit; Singh, R R B; Katara, Antariksh; Rastogi, Subha; Rawat, A K S

    2015-02-01

    The effects of interaction of Asparagus racemosus (shatavari) with milk constituents and physico-chemical and functional characteristics of milk was studied. Addition of freeze dried aqueous shatavari extract at a concentration of 1 g /100 ml of milk showed a decrease in pH, rennet coagulation time and an increase in acidity, viscosity and heat stability at maximum. The extract also imparted brown colour to milk and showed an increase in a* (redness) and b* (yellowness) values but a decrease in L* (lightness) value. Proteins in milk were modified by reaction with shatavari extract. The derivatives formed were characterized in terms of SDS-PAGE. Electrophoretic pattern of sodium caseinate and whey containing 1% shatavari herb extract did not show any difference in band pattern i.e. there was no difference in mobility based on size of the proteins, but the intensity (width) of bands differed. PMID:25694736

  3. Asparagus cochinchinensis Extract Alleviates Metal Ion-Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents.

    Science.gov (United States)

    Zhang, Weiyu; Jin, Li Hua

    2016-01-01

    Metal ions and sulfate are components of atmospheric pollutants that have diverse ways of entering the human body. We used Drosophila as a model to investigate the effect of Asparagus cochinchinensis (A. cochinchinensis) extracts on the gut and characterized gut homeostasis following the ingestion of metal ions (copper, zinc, and aluminum). In this study, we found that the aqueous A. cochinchinensis extract increased the survival rate, decreased epithelial cell death, and attenuated metal ion-induced gut morphological changes in flies following chronic exposure to metal ions. In addition, we screened out, by network pharmacology, six natural products (NPs) that could serve as putative active components of A. cochinchinensis that prevented gut injury. Altogether, the results of our study provide evidence that A. cochinchinensis might be an effective phytomedicine for the treatment of metal ion-induced gut injury. PMID:27123034

  4. Antilithiatic effect of Asparagus racemosus Willd on ethylene glycol-induced lithiasis in male albino Wistar rats.

    Science.gov (United States)

    Christina, A J M; Ashok, K; Packialakshmi, M; Tobin, G C; Preethi, J; Murugesh, N

    2005-11-01

    The ethanolic extract of Asparagus racemosus Willd. was evaluated for its inhibitory potential on lithiasis (stone formation), induced by oral administration of 0.75% ethylene glycolated water to adult male albino Wistar rats for 28 days. The ionic chemistry of urine was altered by ethylene glycol, which elevated the urinary concentration of crucial ions viz. calcium, oxalate, and phosphate, thereby contributing to renal stone formation. The ethanolic extract, however, significantly (p < 0.05) reduced the elevated level of these ions in urine. Also, it elevated the urinary concentration of magnesium, which is considered as one of the inhibitors of crystallization. The high serum creatinine level observed in ethylene glycol-treated rats was also reduced, following treatment with the extract. The histopathological findings also showed signs of improvement after treatment with the extract. All these observations provided the basis for the conclusion that this plant extract inhibits stone formation induced by ethylene glycol treatment. PMID:16357948

  5. Asparagus cochinchinensis Extract Alleviates Metal Ion-Induced Gut Injury in Drosophila: An In Silico Analysis of Potential Active Constituents

    Science.gov (United States)

    Zhang, Weiyu; Jin, Li Hua

    2016-01-01

    Metal ions and sulfate are components of atmospheric pollutants that have diverse ways of entering the human body. We used Drosophila as a model to investigate the effect of Asparagus cochinchinensis (A. cochinchinensis) extracts on the gut and characterized gut homeostasis following the ingestion of metal ions (copper, zinc, and aluminum). In this study, we found that the aqueous A. cochinchinensis extract increased the survival rate, decreased epithelial cell death, and attenuated metal ion-induced gut morphological changes in flies following chronic exposure to metal ions. In addition, we screened out, by network pharmacology, six natural products (NPs) that could serve as putative active components of A. cochinchinensis that prevented gut injury. Altogether, the results of our study provide evidence that A. cochinchinensis might be an effective phytomedicine for the treatment of metal ion-induced gut injury. PMID:27123034

  6. Organic amendments conditions on the control of Fusarium crown and root rot of asparagus caused by three Fusarium spp.

    Directory of Open Access Journals (Sweden)

    Ana I. Borrego-Benjumea

    2015-12-01

    Full Text Available Fusarium oxysporum (Fo, F. proliferatum (Fp and F. solani (Fs are causal agents associated with roots of asparagus affected by crown and root rot, a disease inflicting serious losses worldwide. The propagule viability of Fusarium spp. was determined on substrate artificially infested with Fo5, Fp3 or Fs2 isolates, amended with either poultry manure (PM, its pellet (PPM, or olive residue compost (ORC and, thereafter, incubated at 30 or 35°C for different periods. Inoculum viability was significantly affected by these organic amendments (OAs in combination with temperature and incubation period. The greatest reduction in viability of Fo5 and Fs2 occurred with PPM and loss of viability achieved was higher at 35°C than at 30ºC, and longer incubation period (45 days. However, the viability of Fp3 did not decrease greatly in most of the treatments, as compared to the infested and un-amended control, when incubated at 30ºC. After incubation, seedlings of asparagus `Grande´ were transplanted into pots containing substrates infested with the different species of Fusarium. After three months in greenhouse, symptoms severity in roots showed highly significant decreases, but Fp3 caused lower severity than Fo5 and Fs2. Severity reduction was particularly high at 30ºC (by 15 days incubation for Fs2 and by 30-45 days for Fo5, after PPM treatment, as well as PM-2% for Fo5 and Fs2 incubated during 30 and 45 days at both temperatures, and with ORC (15-30 days incubation. Moreover, assessment of plants fresh weight showed significantly high increases in Fo5 and Fs2, with some rates of the three OAs tested, depending on incubation period and temperature.

  7. Genome wide linkage disequilibrium in Chinese asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm: implications for domestication history and genome wide association studies.

    Science.gov (United States)

    Xu, P; Wu, X; Wang, B; Luo, J; Liu, Y; Ehlers, J D; Close, T J; Roberts, P A; Lu, Z; Wang, S; Li, G

    2012-07-01

    Association mapping of important traits of crop plants relies on first understanding the extent and patterns of linkage disequilibrium (LD) in the particular germplasm being investigated. We characterize here the genetic diversity, population structure and genome wide LD patterns in a set of asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm from China. A diverse collection of 99 asparagus bean and normal cowpea accessions were genotyped with 1127 expressed sequence tag-derived single nucleotide polymorphism markers (SNPs). The proportion of polymorphic SNPs across the collection was relatively low (39%), with an average number of SNPs per locus of 1.33. Bayesian population structure analysis indicated two subdivisions within the collection sampled that generally represented the 'standard vegetable' type (subgroup SV) and the 'non-standard vegetable' type (subgroup NSV), respectively. Level of LD (r(2)) was higher and extent of LD persisted longer in subgroup SV than in subgroup NSV, whereas LD decayed rapidly (0-2 cM) in both subgroups. LD decay distance varied among chromosomes, with the longest (≈ 5 cM) five times longer than the shortest (≈ 1 cM). Partitioning of LD variance into within- and between-subgroup components coupled with comparative LD decay analysis suggested that linkage group 5, 7 and 10 may have undergone the most intensive epistatic selection toward traits favorable for vegetable use. This work provides a first population genetic insight into domestication history of asparagus bean and demonstrates the feasibility of mapping complex traits by genome wide association study in asparagus bean using a currently available cowpea SNPs marker platform.

  8. Genome wide linkage disequilibrium in Chinese asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm: implications for domestication history and genome wide association studies.

    Science.gov (United States)

    Xu, P; Wu, X; Wang, B; Luo, J; Liu, Y; Ehlers, J D; Close, T J; Roberts, P A; Lu, Z; Wang, S; Li, G

    2012-07-01

    Association mapping of important traits of crop plants relies on first understanding the extent and patterns of linkage disequilibrium (LD) in the particular germplasm being investigated. We characterize here the genetic diversity, population structure and genome wide LD patterns in a set of asparagus bean (Vigna. unguiculata ssp. sesquipedialis) germplasm from China. A diverse collection of 99 asparagus bean and normal cowpea accessions were genotyped with 1127 expressed sequence tag-derived single nucleotide polymorphism markers (SNPs). The proportion of polymorphic SNPs across the collection was relatively low (39%), with an average number of SNPs per locus of 1.33. Bayesian population structure analysis indicated two subdivisions within the collection sampled that generally represented the 'standard vegetable' type (subgroup SV) and the 'non-standard vegetable' type (subgroup NSV), respectively. Level of LD (r(2)) was higher and extent of LD persisted longer in subgroup SV than in subgroup NSV, whereas LD decayed rapidly (0-2 cM) in both subgroups. LD decay distance varied among chromosomes, with the longest (≈ 5 cM) five times longer than the shortest (≈ 1 cM). Partitioning of LD variance into within- and between-subgroup components coupled with comparative LD decay analysis suggested that linkage group 5, 7 and 10 may have undergone the most intensive epistatic selection toward traits favorable for vegetable use. This work provides a first population genetic insight into domestication history of asparagus bean and demonstrates the feasibility of mapping complex traits by genome wide association study in asparagus bean using a currently available cowpea SNPs marker platform. PMID:22378357

  9. Selección de progenitores en espárrago Parent selection in asparagus

    Directory of Open Access Journals (Sweden)

    I. Gatti

    2003-06-01

    Full Text Available O objetivo principal deste trabalho foi determinar as variáveis que melhor explicam a variação entre indivíduos dentro da população de aspargos originária da cultivar Argenteüil e selecionar plantas superiores para compor novas populações. O ensaio, composto por 1280 plantas, foi instalado no campo experimental da Facultad de Ciencias Agrarias de la Universidad Nacional de Rosario, Santa Fe, Argentina, em 1996. Durante os anos de 1997 e 1998, foram avaliadas as variáveis: número de turiões por planta (TU; peso médio do turião (PM; diâmetro do turião (DI; produção total por planta (RE; produção comercial por planta (RM; número de dias para início da colheita (DAC; número de hastes por planta (NUTA; altura da haste principal (ALT e peso fresco da massa verde (MASAV. De acordo com a análise de componentes principais, as variáveis RE, RM, PM, DI e TU, são responsáveis por 60% da variação existente dentro da população avaliada. A análise de agrupamento baseada nestas variáveis, permitiu a identificação de cinco grupos distintos para os dois sexos. Os grupos G5 de plantas estaminadas e G5 de plantas pistiladas (7,51% da população total apresentaram as melhores características de RE e qualidade. Destes grupos, foram selecionadas 6 plantas estaminadas e 8 plantas pistiladas para serem utilizadas como genitores no programa de melhoramento de aspargos.The principal aim of this experiment was to ascertain what the variables are which best explain the variations among individuals within the populations of asparagus which originates from the cultivar Argenteüil and as a result select better plants to form new populations. The test, using 1,280 plants, was carried out at he experimental farm in the Facultad Ciencias Agrarias de la Universidad Nacional de Rosario, Santa Fé, Argentina, in 1996. During 1997 and 1998 the number of spears per plant (TU, the mean weight of spears (PM, mean diameter of spears (DI total yield

  10. Confirmation of Fructans biosynthesized in vitro from [1-13C]glucose in asparagus tissues using MALDI-TOF MS and ESI-MS.

    Science.gov (United States)

    Suzuki, Takashi; Maeda, Tomoo; Grant, Suzanne; Grant, Gordon; Sporns, Peter

    2013-05-15

    Accumulation of Fructans was confirmed in asparagus tissues that had been cultured for 2 days on media supplemented with glucose. It is very common that Fructans are biosynthesized from sucrose. We hypothesized however that Fructans could also be biosynthesized from glucose. Stem tissues of in vitro-cultured asparagus were subcultured for 72 h on a medium containing 0.5M of [1-(13)C]glucose. A medium containing 0.5M of normal ((12)C) glucose was used as control. Carbohydrates were extracted from the tissues and analyzed using HPLC, MALDI-TOF MS and ESI-MS. HPLC results indicated that the accumulation of short-chain Fructans was similar in both (13)C-labelled and control samples. Short-chain Fructans of DP=3-7 were detected using MALDI-TOF MS. The molecular mass of each oligomer in the (13)C-labelled sample was higher than the mass of the natural sample by 1 m/z unit per sugar moiety. The results of ESI-MS on the HPLC fractions of neokestose and 1-kestose showed that these oligomers (DP=3) were biosynthesized from exogenous glucose added to the medium. We conclude that not only exogenous sucrose but glucose can induce Fructan biosynthesis; fructans of both inulin type and inulin neoseries are also biosynthesized from glucose accumulated in asparagus tissues; the glucose molecules (or its metabolic products) were incorporated into Fructans as structural monomers.

  11. 3个芦笋品种产量及农艺性状的研究%Study on yields and agronomic characters of three different asparagus species

    Institute of Scientific and Technical Information of China (English)

    谷永丽; 杨恒山; 王红霞

    2015-01-01

    In experimental farm of Inner Mongolia University for Nationalities, eleven years three asparagus species (Apollo and Grande and Atlas) as experimental material, the yields and agronomic characters of different asparagus species were studied in 2015. The results were as follow: In three experimental species, the yield of different grades asparagus of Grande was highest, average output rate per day;weight of per plant;stem number per plant; and branches per plant of Grande were also highest. In general, grande was much better than others.%2015年在西辽河平原的内蒙古民族大学试验农场,以11年生的阿波罗、格兰德、阿特拉斯3个芦笋品种为试验材料,研究了不同芦笋品种的产量及农艺性状。结果表明:在3个品种中,格兰德品种的各级笋产量均最高,日均产笋速率、单支重、笋径及单株采笋支数均最高。

  12. ITS序列在天门冬属物种DNA条形码构建中的应用研究%Application research of ITS sequences DNA barcoding in asparagus species

    Institute of Scientific and Technical Information of China (English)

    蒋向辉; 曾桂萍; 欧立军; 佘朝文; 许栋

    2011-01-01

    在对天门冬属4个种ITS(internal transcribed spacer)序列克隆测序的基础上,并对4个种进行序列比较分析.结果表明:(1)4个物种的亲缘关系很近,它们间的ITS序列的变异小,GC含量高,在60%左右.(2)4个物种在系统树上聚为一类,文竹与其他3种天门冬的亲缘关系相对较远,处于系统树的一支,其中,非洲天门冬与新疆天门冬的亲缘关系较近,处于系统树的一支.(3) ITS序列的种间差异值略有不相,短梗天门冬与非洲天门冬的差异值为2.8%,与新疆天门冬的差异值为3.5%,与文竹的差异值为8.5%,非洲天门冬与新疆天门冬的差异值为2.1%,与文竹的差异值为8.3%,新疆天门冬与文竹的差异值为9.1%.综合分析认为:以ITS序列为DNA条形码可真实反应天门冬属物种间差异.%This study made cluster analysis among the 4 spices of Asparagus, based on ITS (internal transcribed spacer) cloning sequencing. Firstly the result showed that there has close relationship and little variation of ITS sequence among 4 spices, with the highly 60% of GC content. Secondly, four spices belong to one kind, while Asparagus setaceus, in one branch of pylogenentic tree, has far genetic relationship with the other 3 Asparagus Species. And thirdly the result was in line with lianghu qu etal got . Once he got the interspesfic difference value of most family and genus ITS sequence of angio-sperm varies from 1. 2 % to 10. 2 % via comparison with different live groups,and inter-generic difference value fluctuates from 9. 6 % to 28. 8 %. The interspecific difference value between Asparagus lycopodineus and Asparagus densiflorus, Asparagus neglec-tus, Asparagus setaceus is as follows; 2.8%, 3. 5%, 8. 5%. The difference value between Asparagus densiflorus and Asparagus neglectus, Asparagus setaceus is as follows :2. 1% and 8. 3%. The difference value between Asparagus neglectus and Asparagus setaceus is 9. 1%. Our analysis suggests ITS sequences

  13. Present Situation,Problems and Countermeasures of Chongming Asparagus Industry%崇明芦笋产业发展现状、问题及对策建议

    Institute of Scientific and Technical Information of China (English)

    陈泉生; 孟凡磊

    2014-01-01

    崇明芦笋产业经过30a的发展,已形成种植面积近667hm2,年产值超亿元的规模,是当地农民增收的主栽品种。该文从崇明芦笋产业现状出发,结合国内外芦笋市场需求,通过调研,分析了崇明芦笋产业发展存在的问题,提出了确保崇明芦笋产业健康发展的对策建议,为实现芦笋产业的可持续发展提供参考。%Chongming Asparagus industry has formed a considerable scale that the plant area is nearly 667hm2 and annual output value is over one hundred million yuan after more than 30 years of develop-ment. As a result,Asparagus has become the main cultivar to increase the income of local farmers. The study embarks from the present situation of Chongming Asparagus industry,combining with market demand both at home and abroad to analyse the problems in the development of chongming Asparagus industry and put forward countermeasures and suggestions to ensure the healthy and sustainable development of As-paragus industry.

  14. Ovicidal, larvicidal and adulticidal properties of Asparagus racemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis (Culex quinquefasciatus), dengue (Aedes aegypti) and malaria (Anopheles stephensi) vector mosquitoes (Diptera: Culicidae).

    Science.gov (United States)

    Govindarajan, Marimuthu; Sivakumar, Rajamohan

    2014-04-01

    Several diseases are associated to the mosquito-human interaction. Mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, West Nile virus and yellow fever. These diseases produce significant morbidity and mortality in humans and livestock around the world. The present investigation was undertaken to study the ovicidal, larvicidal and adulticidal activities of crude hexane, ethyl acetate, benzene, chloroform and methanol extracts of root of Asparagus racemosus were assayed for their toxicity against three important vector mosquitoes, viz., Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). The mean percent hatchability of the eggs was observed after 48 h post-treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the methanol extract showed the highest ovicidal activity. The methanol extract of Asparagus racemosus against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi exerted 100% mortality (zero hatchability) at 375, 300 and 225 ppm, respectively. Control eggs showed 99-100% hatchability. The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in methanol extract of root of Asparagus racemosus against the larvae of Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi with the LC50 and LC90 values were 115.13, 97.71 and 90.97 ppm and 210.96, 179.92, and 168.82 ppm, respectively. The adult mortality was observed after 24 h recovery period. The plant crude extracts showed dose-dependent mortality. At higher concentrations, the adult showed restless movement for some times with abnormal wagging and then died. Among the extracts tested, the highest adulticidal activity was observed in

  15. 土壤保水剂在芦笋上的应用效果%Application Effects of Soil Water -retaining Agent in Asparagus

    Institute of Scientific and Technical Information of China (English)

    徐宏; 史学明; 黄志勇; 谢晟; 罗绍春

    2011-01-01

    The application effects of new type soil water - retaining agent, which was synthesized through irradiation, in asparagus were studied. The results of pot experiments indicated that the use of water - retaining agent could increase the stem number of asparagus effectively, and enhance the stem width; comparing with the control ( CK) , the growth index of asparagus in water - retaining agent wet - application treatment was increased by 53.9% ~ 77.0% , and that in dry - application treatment was increased by 5.8% ~ 38.7%. The results of field tests showed that the plant height, plant number, stem width and growth index of asparagus in wet - application treatment were increased by 14.2% , 12.5% , 23.8% and 59.0% respectively as compared with those of CK; the yield of asparagus in wet - application treatment was 0.6% and 32.7% higher than that in dry - application treatment and CK, respectively.%研究了辐照合成新型土壤保水剂在芦笋上的应用效果.盆栽试验结果表明:施用保水剂可有效增加芦笋茎数,提高芦笋茎粗,湿施处理生育指数比对照增加53.9%~77.0%,干施处理生育指数比对照增加5.8%~38.7%.大田试验结果表明:湿施保水剂的芦笋株高、株数、茎粗和生育指数分别比对照提高14.2%、12.5%、23.8%、59.0%,湿施保水剂的大田芦笋产量比干施和对照分别提高0.6%和32.7%.

  16. Germination and vigour of primed asparagus seeds Germinação e vigor de sementes de aspargo osmoticamente condicionadas

    Directory of Open Access Journals (Sweden)

    Mário Lúcio de Carvalho Bittencourt

    2005-08-01

    Full Text Available The osmotic conditioning or priming is considered a promising technique to speed up germination and to improve seed performance. Four seed lots of asparagus, Mary Washington cultivar, were primed at 25°C for seven or 14 days using PEG 6000 at -1.0 or -1.2 MPa, or sea water at -3.3 MPa; or for three days in distilled water. The physiological quality of the seeds was evaluated by standard germination, first count germination, speed of seedling emergence, and germination percentage and seedling fresh and dry weights after controlled deterioration test. Primed seeds presented higher germination speed, independently of their initial physiological quality. Beneficial effects of priming on germination and vigour were more expressive in the seed lot of low physiological quality. Priming in PEG 6000 at -1.0 MPa for 14 days was the most beneficial treatment to improve asparagus seeds performance.O condicionamento osmótico ou priming tem sido considerado uma técnica promissora para acelerar a germinação e melhorar o desempenho das sementes. Sementes de quatro lotes de aspargo, cv. Mary Washington, foram condicionadas em PEG 6000 a -1,0 e -1,2 MPa, por 7 e 14 dias, em água do mar a -3,3 MPa, por 7 e 14 dias e em água destilada por 3 dias, em BOD a 25°C. Sementes não condicionadas foram usadas como testemunha. A qualidade fisiológica das sementes foi avaliada pela germinação, primeira contagem de germinação, velocidade de emergência e pela porcentagem de geminação e pesos da matéria fresca e seca das plântulas obtidas no teste de deterioração controlada (45°C/24 horas. O experimento foi instalado no delineamento inteiramente casualizado com quatro repetições e oito tratamentos, sendo as médias comparadas pelo teste de Duncan a 5%. Sementes de aspargo condicionadas osmoticamente apresentaram maior velocidade de germinação, independentemente de sua qualidade fisiológica inicial. Efeitos benéficos do condicionamento osmótico, na

  17. The maximum residue limits and determination of pesticide residues in asparagus%芦笋中农药最大残留限量及检测方法研究进展

    Institute of Scientific and Technical Information of China (English)

    宋欢; 车兰兰; 林勤保; 王蓉珍

    2012-01-01

    总结了芦笋中常用农药及欧盟、日本、美国和我国对其最大残留限量规定,并对农药残留相关检测方法在芦笋等果蔬中的应用进行综述,以期为我国芦笋中农药残留检测和控制提供参考。%Common pesticides used in asparagus planting and the maximum residue limits (MRLs) of those in European Union,Japan,USA and China were summarized,and also the application of relevant determination methods of pesticide residues to asparagus ,vegetables and fruit were reviewed. The paper is aimed at providing reference for pesticide residue determination and controlling in Chinese asparagus field.

  18. 芦笋多糖对S180小鼠红细胞补体受体-1(CR1,CD35)数量及活性的影响%Effect of Asparagus Polysaccharide on the Number and Activity of Erythrocyte Complement Receptor 1(CD35) of S180 Mice

    Institute of Scientific and Technical Information of China (English)

    季宇彬; 闵永萃; 汲晨锋

    2009-01-01

    目的 研究探讨芦笋多糖对S180小鼠红细胞补体受体-1(CR1)数量、红细胞CR1天然免疫活性的影响.方法 取小鼠的新鲜红细胞,用大鼠抗小鼠CD35一抗和FITC-羊抗大鼠二抗标记小鼠红细胞,应用流式细胞仪测定小鼠红细胞CR1数量;用红细胞免疫花环实验检测红细胞C3b受体花环率(RBC-C3bRR)和肿瘤红细胞细胞花环率(DTER).结果 芦笋多糖高剂量组和中剂量组使S180小鼠红细胞CR1数量和RBC-C3bRR、DTER显著性增加.结论 芦笋多糖可以改善S180小鼠红细胞免疫功能,可能是其抗肿瘤作用的机制之一.%OBJECTIVE To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor-1 in S 180 mice. METHODS Red blood cells from mice venous blood were labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, the number of ECR1 was determined. Using microscope, the adherence between erythrocyte immunity and C3b receptor or tumor-cell was investigated by RBC-C3bRR and DTER. RESULTS Comparing with control groups, the mean value of the number of CR1, RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups were increased significantly. CONCLUSION Asparagus officinalis polysaccharide can improve the erythrocyte function of S 180 mice, which may be one of its most important antitumor mechanisms.

  19. 冰温贮藏对绿芦笋品质及酶活性的影响%Effect of ice-temperature preservation on quality and enzymatic activity of Green Asparagus

    Institute of Scientific and Technical Information of China (English)

    宋秀香; 鲁晓翔; 陈绍慧; 李江阔

    2013-01-01

    To 4℃ refrigerated storage for comparison,the effect of ice-temperature preservation on quality and enzymatic activity of Green Asparagus was investigated.The results showed that the storage life of Green asparagus was increased 14d,and its organoleptic quality was improved by ice-temperature preservation.While the firmness and tincture of Green asparagus was kept,the decrease of the V c content was slowed down,and its commodity value was improved by ice-temperature preservation.The activity of PPO and POD of Green asparagus was increased,and the activity of PAL was decreased under ice-temperature.So the protection ability of Green asparagus was enhanced,and the aging of Green asparagus was delayed.The control effect of ice-temperature preservation on respiration and ethylene production was unconspicuous.%以4℃冷藏作为对照,研究了冰温贮藏对绿芦笋品质及酶活性的影响.结果表明:冰温贮藏延长了绿芦笋贮藏期14d,提高其感官品质;同时,冰温贮藏保持了绿芦笋的硬度和色泽,减缓了Vc含量的降低,提高了其商品价值;冰温条件下绿芦笋多酚氧化酶(PPO)和过氧化物酶(POD)的活性提高,苯丙氨酸解氨酶(PAL)活性降低,增强了自身保护能力,延缓绿芦笋衰老;但冰温贮藏对绿芦笋呼吸作用和乙烯生成量的抑制效果不明显.

  20. Safety Evaluation of Different Kinds of Herbicides on Asparagus Seedlings%不同类型除草剂对苗期芦笋的安全性评价

    Institute of Scientific and Technical Information of China (English)

    赵聚莹; 贾海民; 蒋晓丽; 陈丹

    2014-01-01

    To choose the best herbicides types for controlling weeds in asparagus seedling field and determine its safety evaluation for asparagus, experimental studied the influence on asparagus seedling of seven pre-emergence spraying herbicides and seven post-emergence herbicides.The results showed that 330 g/L pendimethalin EC (750 mL/hm2) had little influence on the emergence of asparagus, 15% fluazifop-P EC (500 mL/hm2) had no significant phytotoxicity on asparagus seedling.The two kinds of herbicides could be used in asparagus seedling fields, and other herbicides could cause different extents of phytotoxicity.%为探索防治芦笋育苗田杂草的适宜除草剂类型及其对苗期芦笋的安全性,利用花盆种植芦笋,在温室内分别进行了7种封闭类除草剂和7种苗后除草剂对芦笋幼苗生长的安全性评价。结果表明:播后苗前施用330 g/L二甲戊灵乳油750 mL/hm2对芦笋出苗影响不明显,苗后施用15%精吡氟禾草灵乳油500 mL/hm2对芦笋幼苗生长无明显的药害症状,2种除草剂均可以在芦笋育苗田使用。

  1. Toxicological assessment of enzyme-treated asparagus extract in rat acute and subchronic oral toxicity studies and genotoxicity tests.

    Science.gov (United States)

    Ito, Tomohiro; Ono, Tomoko; Sato, Atsuya; Goto, Kazunori; Miura, Takehito; Wakame, Koji; Nishioka, Hiroshi; Maeda, Takahiro

    2014-03-01

    The safety of enzyme-treated asparagus extract (ETAS) developed as a novel anti-stress functional material was assessed in acute and subchronic studies and genotoxicity assays. In the acute oral dose toxicity study, all rats survived during the test period and ETAS did not influence clinical appearance, body weight gain and necropsy findings at a dosage of 2000mg/kg body weight. Thus, the 50% lethal dose (LD50) of ETAS was determined to be greater than 2000mg/kg. The 90-day subchronic study (500, 1000 and 2000mg/kg body weight, delivered by gavage) in rats reported no significant adverse effects in food consumption, body weight, mortality, urinalysis, hematology, biochemistry, necropsy, organ weight and histopathology. In the micronucleus test of mice, the incidence of micronuclei in ETAS-administered groups (500, 1000 and 2000mg/kg/day, injected twice) was equivalent to that of the negative control group, while the positive control group receiving mitomycin C showed a high incidence. The potential of ETAS to induce gene mutation was tested using four Salmonella typhimurium strains and Escherichia coli WP2uvrA. The test sample was not mutagenic to the test strains. These results support the safety of ETAS as food and dietary supplement.

  2. Ameliorative effect of Asparagus racemosus root extract against pentylenetetrazol-induced kindling and associated depression and memory deficit.

    Science.gov (United States)

    Pahwa, Priyanka; Goel, Rajesh Kumar

    2016-04-01

    Asparagus racemosus (A. racemosus) roots are extensively used in traditional medicine for the management of epilepsy. The aim of the present study was to investigate the ameliorative effect of A. racemosus root extract (ARE) against pentylenetetrazol-induced kindling and associated depression and memory deficit. Kindling was successfully induced by repeated administration of a subconvulsant dose of PTZ (35 mg/kg; i.p.) at an interval of 48 ± 2 h in 43 days (21 injections). Pretreatment with valproate (300 mg/kg; i.p.), a major antiepileptic drug as well as ARE significantly suppressed the progression of kindling. Moreover, ARE also ameliorated the kindling-associated depression and memory deficit as indicated by decreased immobility time and increased step-down latency, respectively, as compared to vehicle control animals. Further, these behavioral observations were complemented with analogous neurochemical changes. In conclusion, the results of the present study showed that ARE treatment has an ameliorative effect against PTZ-induced kindling and associated behavioral comorbidities. PMID:26970996

  3. Protection against experimental visceral leishmaniasis by immunostimulation with herbal drugs derived from Withania somnifera and Asparagus racemosus.

    Science.gov (United States)

    Kaur, Sukhbir; Chauhan, Kalpana; Sachdeva, Heena

    2014-10-01

    Visceral leishmaniasis (VL) is a vector-borne parasitic disease targeting tissue macrophages. It is among the most neglected infectious diseases. As available therapeutics for treatment of this disease have many side effects, there is a need for safer alternatives. One of the immunopathological consequences of active visceral leishmaniasis is suppression of protective T-helper (Th)-1 cells and induction of disease-promoting Th-2 cells, and thus the treatment of VL relies on immunomodulation. In the current study, herbal drugs derived as whole-plant extracts of Asparagus racemosus and Withania somnifera were used to treat Leishmania donovani-infected BALB/c mice. Keeping the scenario of immunosuppression during VL in mind, the potential of these drugs in the restoration of murine Th-1-type protective immune responses was evaluated. To investigate the propensity of these drugs to treat VL, liver parasite load, delayed-type hypersensitivity responses and parasite-specific immunoglobulin levels were studied. Various biochemical and haematological tests were also carried out. A positive-control group used the standard drug treatment of sodium stibogluconate. Treatment of infected mice with A. racemosus and W. somnifera in combination at the higher dose of 200 mg (kg body weight)(-1) not only resulted in a successful reduction in parasite load but also generated protective Th1-type immune responses with normalization of biochemical and haematological parameters, suggesting their potential as potent anti-leishmanial agents.

  4. Optimization for SRAP-PCR System of Asparagus Offcinalis L.Based on Orthogonal Design%利用正交设计优化芦笋SRAP反应体系

    Institute of Scientific and Technical Information of China (English)

    李霞; 于继庆; 李书华; 杨林; 牟萌

    2011-01-01

    以芦笋(Asparagus officinalis L.)为材料,对影响SRAP-PCR扩增结果的因素dNTPs、Taq DNA聚合酶、Mg2+、引物、模板DNA进行了正交优化设计的研究.结果表明,适合芦笋SRAP-PCR分析的最佳反应体系:20μL的反应体积中包括0.2 mmol/L dNTPs 0.4μL;1 U Taq DNA酶1.0μL;1.5 mmol/L Mg2+1.2μL;上下引物各30 ng,每个引物0.5μL;60 ng模板DNA 1.0μL;10×Buffer 2.0μL;无菌双蒸水13.4μL.为今后利用SRAP标记技术研究芦笋的遗传多样性奠定基础.

  5. 两种典型保鲜剂对芦笋保鲜效果的研究%Effect on the Quality of Asparagus in Cold Storage with 6-BA and Chitosan

    Institute of Scientific and Technical Information of China (English)

    张喜才; 谢晶

    2007-01-01

    绿芦笋(Asparagus officinalis L.cv jersey Giant)是一种营养丰富,并具有一定药用价值的名贵蔬菜,特别对癌症的防治有较好的功效.由于绿芦笋嫩茎含水量较高,呼吸作用强,采收后不耐贮藏,因而给鲜销带来很多麻烦.试验采取2种典型保鲜剂(壳聚糖和6-BA)对绿芦笋进行后处理,并定期检测样品的失质量率、VC含量、叶绿素含量、可溶性固形物含量和纤维素5个指标.实验结果证明,这2种保鲜剂不仅都能很大程度地延长绿芦笋的贮藏时间,而且还有各自的特性,在选择短期或长期芦笋储运时可提供不同的选择.

  6. Molecular analysis of the genus Asparagus based on matK sequences and its application to identify A. racemosus, a medicinally phytoestrogenic species.

    Science.gov (United States)

    Boonsom, Teerawat; Waranuch, Neti; Ingkaninan, Kornkanok; Denduangboripant, Jessada; Sukrong, Suchada

    2012-07-01

    The plant Asparagus racemosus is one of the most widely used sources of phytoestrogens because of its high content of the steroidal saponins, shatavarins I-IV, in roots. The dry root of A. racemosus, known as "Rak-Sam-Sip" in Thai, is one of the most popular herbal medicines, used as an anti-inflammatory, an aphrodisiac and a galactagogue. Recently, the interest in plant-derived estrogens has increased tremendously, making A. racemosus particularly important and a possible target for fraudulent labeling. However, the identification of A. racemosus is generally difficult due to its similar morphology to other Asparagus spp. Thus, accurate authentication of A. racemosus is essential. In this study, 1557-bp nucleotide sequences of the maturase K (matK) gene of eight Asparagus taxa were analyzed. A phylogenetic relationship based on the matK gene was also constructed. Ten polymorphic sites of nucleotide substitutions were found within the matK sequences. A. racemosus showed different nucleotide substitutions to the other species. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the matK gene was developed to discriminate A. racemosus from others. Only the 650-bp PCR product from A. racemosus could be digested with BssKI into two fragments of 397 and 253-bp while the products of other species remained undigested. Ten commercially crude drugs were analyzed and revealed that eight samples were derived from A. racemosus while two samples of that were not. Thus, the PCR-RFLP analysis of matK gene was shown to be an effective method for authentication of the medicinally phytoestrogenic species, A. racemosus.

  7. Heavy metal accumulations of 24 asparagus bean cultivars grown in soil contaminated with Cd alone and with multiple metals (Cd, Pb, and Zn).

    Science.gov (United States)

    Zhu, Yun; Yu, Hui; Wang, Junli; Fang, Wei; Yuan, Jiangang; Yang, Zhongyi

    2007-02-01

    Crops grown in heavy metal contaminated soils are an important avenue for these toxic pollutants entering the human food chain. Information on how crops respond to soil contaminations of single versus multiple metals is scarce and much needed. This study investigated the accumulation of Cd by 24 cultivars of asparagus bean (Vigna unguiculata subsp. Sesquipedalis L., family Fabaceae) under a low level (0.8 mg kg-1) and a high level (11.8 mg kg-1) of Cd exposure in a garden experiment, and that in a field experiment with Cd, Pb, and Zn (1.2, 486, and 1114 mg kg-1, respectively) contaminated soil. Both experiments showed that there were highly significant variations among the tested cultivars in Cd accumulation by roots, stems, leaves, and fruits of asparagus bean. In the garden experiment, all cultivars under the low Cd exposure and 41.7% of the tested cultivars under the high Cd exposure bore fruits (pods) whose Cd concentrations were lower than 0.05 mg kg-1 fw and therefore were safe for consumption. In addition, the fruit Cd concentrations of cultivars with black seed coats were significantly lower than those with red or spotted seed coats. These results suggest that asparagus bean is a hypo-accumulator to Cd pollutant and the trait of Cd accumulation is genetic-dependent among cultivars. In the field experiment, correlation between fruit Cd and Pb concentrations was significantly positive (p pollution-safe-cultivars (PSC) in multi-metal polluted condition could refer to the PSCs selected under a high level exposure of a single heavy metal.

  8. 上海地区石刁柏设施栽培法对采收和茎枯病害发生的影响%EFFECT OF PROTECTED CULTURE MODES ON PICKING YOUNG STALK AND STEM BLIGHT INCIDENCE OF ASPARAGUS IN SHANGHAI

    Institute of Scientific and Technical Information of China (English)

    陈泉生; 杨瑾华; 马飞; 杨锋; 陆美英

    2003-01-01

    通过设施栽培试验,结果表明石刁柏(Asparagus officinalis L.)的产量比露地常规栽培提高15.8%,一级品比率提高89.0%,产值增加144.2%,采收期延长90d.由于设施栽培避开了自然雨水对石刁柏的直接影响,降低了田间湿度,在减少病害防治次数及用药量的同时,茎枯病平均发病率从74.8%下降到10.0%.

  9. Study on Ecological Adaptability and High -efficient Technology of Asparagus Production in Nantong%南通市芦笋生产的生态适应性及高效种植技术途径研究

    Institute of Scientific and Technical Information of China (English)

    刘建; 魏亚凤; 杨美英; 季桦

    2012-01-01

    从温度、光照、土壤水分和空气湿度、土壤、不良环境与劣质异形笋的发生等方面,分析了芦笋生长的适宜环境.根据温光资源条件和水土环境特征,明确了南通市芦笋生产的生态适应性.提出了南通市芦笋生产的高效种植技术途径和播种育苗、移栽定植、田间管理等标准化生产技术.%This paper analyzed the suitable environment for the growth of asparagus from the aspects of temperature, light, soil moisture, air humidity, soil, bad environment, the occurrence of inferior abnormal asparagus and so on. According to the temperature and light conditions as well as the water and soil environmental characteristics, the ecological adaptability of asparagus production in Nantong city was nailed down. The high - efficient cultivation technology of asparagus in Nantong city and its some standardized technologies of seeding, seedling - raising, transplanting and field management were also pointed out.

  10. Utilization Status and High-efficient Development Countermeasures of Asparagus Waste Straws%芦笋废弃秸秆利用现状与高效开发对策

    Institute of Scientific and Technical Information of China (English)

    胡新龙; 张岳平; 陈光宇

    2015-01-01

    简述了芦笋废弃秸秆的利用价值及在动物饲料、食用菌培养、食品工业、沼气应用和土壤改良等方面的研究利用现状,并有针对性地提出了几点高效开发对策,以期为芦笋产业的进一步发展提供参考。%In this paper, the utility value of garden asparagus waste straws was briefly reviewed , the current research and utili-zation statuses of asparagus waste straws in animal feed , edible mushroom culture, food industry, marsh gas use and soil improve-ment were summarized, and several high -efficient development countermeasures of asparagus waste straws were pointedly put for -ward, in order to provide a reference for the further development of asparagus industry in future .

  11. 海芦笋膳食纤维提取技术%Extraction Techniques of Dietary Fiber from Sea Asparagus

    Institute of Scientific and Technical Information of China (English)

    张斌; 王朋; 何键东; 罗红宇

    2011-01-01

    [Objective] The objective was to study the technology condition for extracting the dietary fiber from the sea asparagus and prepare the soluble and insoluble dietary fiber. [ Method ] The method of combining the enzyme and chemical was used to prepare the 2 kinds of dieta ry fiber. [ Result ] Under the conditions as pH in cellulose of 4 - 5, pH in neutral protease solution of 6 - 7, temperature of 48- 50 ℃, cellu lose of 800 U/g, protease of 2 000 U/g, enzyme solution time of 2 h, the best enzymatic hydrolysis results were obtained. The isolated soluble dietary fiber and insoluble dietary fiber were obtained after extracting the crude fiber with 2.25% sodium carbonate solution (40 times) for 1h at 50 t. The insoluble dietary fiber was first boiled with 0. 75% sodium hydroxide solution for 30 min, then with 1.5% hydrochloric acid so lution for 20 min. The insoluble dietary fiber could be mixed with the soluble dietary fiber. [ Conclusion] The preparation of the dietary fiber from the sea asparagus under the optimized process could get the yield of 21.28% , in which the ratio of soluble dietary and fiber insoluble di etary fiber was 1:3.%[目的]研究从海芦笋提取膳食纤维的工艺条件,并制备可溶性和不溶性膳食纤维.[方法]采用酶与化学法相结合的方法,制备2种膳食纤维.[结果]纤维素酶pH4 ~5,中性蛋白酶pH 6 ~7,温度均为48~50℃,纤维素酶800 U/g,蛋白酶2 000 U/g,酶解时间各为2h的条件下,获得最佳酶解效果;酶解后的粗纤维用40倍2.25% Na2CO3溶液,在50℃条件下提取1h,分离得到可溶性和不溶性膳食纤维,不溶性膳食纤维先用0.75% NaOH溶液碱煮30 min,再用1.5% HCl溶液酸煮20 min,将可溶性和不溶性膳食纤维混合.[结论]优化工艺下制备海芦笋膳食纤维,得率为21.28%,其中可溶性膳食纤维∶不可溶性膳食纤维为1∶3.

  12. A New Asparagus Bean Cultivar ‘Fengchan 6'%长豇豆新品种‘丰产6号’

    Institute of Scientific and Technical Information of China (English)

    李桂花; 陈汉才; 张艳

    2012-01-01

    ‘Fengchan 6' is a new earlymedium systematic breeding cultivar which comes from Zengcheng White Asparagus variant. It is characterized by vigorous growth, elite quality, high yield, insensitivity to light period and resistance to diseases. The pod is light green with about 60 cm long, 1.0 cm wide and 28 g in weight. It is suitable in main asparagus bean growing region in spring, summer, autumn in Guangdong Province.%长豇豆早中熟新品种‘丰产6号’是由‘增城花仁白豆’的变异株系统选育而成。荚色绿白,荚条直,肉厚,荚长60cm,荚宽1.0cm,单荚质量28g。荚形整齐,荚面平整,纤维少,品质优,产量高,对光周期反应不敏感,田间枯萎病、锈病发病率低,适宜广东省春夏秋季种植。

  13. The 2b protein of Asparagus virus 2 functions as an RNA silencing suppressor against systemic silencing to prove functional synteny with related cucumoviruses.

    Science.gov (United States)

    Shimura, Hanako; Masuta, Chikara; Yoshida, Naoto; Sueda, Kae; Suzuki, Masahiko

    2013-08-01

    Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus in the family Bromoviridae. We cloned the coat protein (CP) and the 2b protein (2b) genes of AV-2 isolates from asparagus plants from various regions and found that the sequence for CP and for 2b was highly conserved among the isolates, suggesting that AV-2 from around the world is almost identical. We then made an AV-2 infectious clone by simultaneous inoculation with in vitro transcripts of RNAs 1-3 of AV-2 and in vitro-synthesized CP, which is necessary for initial infection. Because 2b of cucumoviruses in Bromoviridae can suppress systemic silencing as well as local silencing, we analyzed whether there is functional synteny of 2b between AV-2 and cucumovirus. Using the AV-2 infectious clone, we here provided first evidence that Ilarvirus 2b functions as an RNA silencing suppressor; AV-2 2b has suppressor activity against systemic silencing but not local silencing.

  14. Cladodes, leaf-like organs in Asparagus, show the significance of co-option of pre-existing genetic regulatory circuit for morphological diversity of plants.

    Science.gov (United States)

    Nakayama, Hokuto; Yamaguchi, Takahiro; Tsukaya, Hirokazu

    2012-08-01

    Plants in the genus Asparagus have determinate leaf-like organs called cladodes in the position of leaf axils. Because of their leaf-like morphology, axillary position, and morphological variation, it has been unclear how this unusual organ has evolved and diversified. In the previous study, we have shown that cladodes in the genus Asparagus are modified axillary shoots and proposed a model that cladodes have arisen by co-option and deployment of genetic regulatory circuit (GRC) involved in leaf development. Moreover, we proposed that the alteration of the expression pattern of genes involved in establishment of adaxial/abaxial polarity has led to the morphological diversification from leaf-like to rod-like form of cladodes in the genus. Thus, these results indicated that the co-option and alteration of pre-existing GRC play an important role in acquisition and subsequent morphological diversification. Here, we present data of further expression analysis of A. asparagoides. The results suggested that only a part of the GRC involved in leaf development appears to have been co-opted into cladode development. Based on our study and several examples of the morphological diversification, we briefly discuss the importance of co-option of pre-existing GRC and its genetic modularity in the morphological diversity of plants during evolution.

  15. 无糖天冬蜜饯加工工艺研究%Processing Technology of Sugar-free Preserved Asparagus

    Institute of Scientific and Technical Information of China (English)

    詹永; 杨勇; 祝卢艺

    2011-01-01

    Processing technology of sugar-free preserved Asparagus was studied.Single factor experiments showed that 0.1% CaCl2 hardening 15 h,50 ℃~55 ℃ vacuum drying 12 h.Sugar manufacturing combined with cooked and dipped,orthogonal experiments showed that optimal sugar manufacturing conditions were 20% of the initial concentration of xylitol,sugar cooking time of 10 min,soaked sugar time 24 h,citric acid 0.20%.The sugar-free preserved Asparagus is full of winter white,sweet and sour appropriate,cool and refreshing,unique flavor.%对无糖天冬蜜饯的加工工艺进行了研究。单因素试验表明0.1%CaCl2硬化15 h,50℃~55℃真空干燥12 h。糖制采用煮浸结合,正交试验表明糖制最佳条件为木糖醇起始浓度20%、煮糖时间10 min、浸糖时间24 h、柠檬酸用量0.20%。制成的无糖天冬蜜饯饱满洁白、酸甜适宜、清凉爽口、风味独特,特别适宜于糖尿病人食用。

  16. Effects of Arbuscular Mycorrhizal Fungi on Mineral Element Absorption and Nutrient Quality of Asparagus in Plastic Tunnel%AM真菌对大棚芦笋矿质元素吸收和营养品质的影响

    Institute of Scientific and Technical Information of China (English)

    于二敏; 李聪; 贺超兴

    2014-01-01

    Effects of inoculation of arbuscular mycorrihizal fungi Glomus mosseae(GM)on biomass, mineral element absorption and nutrient quality of asparagus(Asparagus officialis L.)in plastic tunnel.The results showed that the fresh weight of GM inoculated plant increased by 45.57%than non-inoculated control,and the dry matter weight of GM inoculated plant increased by 29.65%.GM inoculation obviously increases the content of chlorophyll and carotenoids in asparagus cladophyll and strengthens the root activity.The absorption abilities for N,P,K,Mg,Mn,Cu in inoculated asparagus are also increased.The contents of rutin and saponins in GM inoculated asparagus are significantly increased than the control.%将接种丛枝菌根真菌摩西球囊霉(Glomus mosseae,GM)的芦笋菌根苗定植在大棚内,研究菌根对芦笋植株生物量、矿质元素吸收和产量品质的影响。结果表明:接种GM的芦笋单株地上部鲜质量增加了45.57%,地上部干质量增加了29.65%;拟叶叶绿素、类胡萝卜素含量和植株根系力均显著增加;植株吸收N、P、K、Mg、Mn、Cu的能力均有不同程度地提高;嫩中芦丁含量和皂苷含量也显著提高。

  17. Nouveau regard sur le comportement de l'asperge (Asparagus officinalis L.) multipliee in vitro

    OpenAIRE

    Dore, Claire

    1988-01-01

    La possibilité de multiplier végétativement in vitro l’asperge a permis notamment de cloner les parents des variétés hybrides de clones. L’obtention d’une griffe fonctionnelle dès le stade in vitro est un facteur essentiel de réussite de la micropropagation. Des améliorations permettant de maîtriser la méthode de clonage sont proposées ici. Une série de microbouturages est nécessaire pour induire ultérieurement de nombreux bourgeons qu’il est possible de fragmenter comme des griffes. Le...

  18. Pharmacognostical Identification of Asparagus officinalis L.%芦笋的生药学鉴定

    Institute of Scientific and Technical Information of China (English)

    揭晶; 殷丹; 刘焰

    2014-01-01

    目的 对芦笋进行生药学鉴定,为该药的开发利用及质量标准的制定提供科学依据.方法 采用性状鉴别、显微鉴别、理化鉴别的方法.结果 芦笋药材性状、显微鉴别和理化鉴别具有专属性特征.结论 该方法简便易操作,可作为芦笋鉴定的依据.

  19. Impact of Mooring Asparagus Angle on Towing of Ocean Platform with Bucket Foundation%系缆龙须角对筒型基础拖航影响试验

    Institute of Scientific and Technical Information of China (English)

    刘宪庆; 杨子慧; 丁红岩; 辜文杰

    2014-01-01

    系缆龙须角是影响气浮筒型基础拖航的主要因素之一。通过模型试验对顺浪拖航时结构在横向、纵向以及垂向振荡的加速度、筒内气压力和水压力以及拖缆力进行测量。试验结果表明:拖航加速度随着系缆龙须角从0°增大到90°呈先减小后增大的趋势;龙须角为0°时,拖航的加速度、筒内气压力和水压力以及拖缆力变化均明显大于有角度拖航;拖缆力呈脉冲变化,且在45°时取得较小值。因此对于该结构的拖航,为了保证在拖航中既具有较小的拖缆力,又具有较小的横向和垂向振荡,建议选取系缆龙须角为45°。%The mooring asparagus angle is one of the main factors which affect the towing of air-floating bucket foundations. The accelerations of longitudinal, lateral, vertical acceleration, the internal air pressure and water pressure of the bucket and the tension of towing are determined by towing in following wave through model test. The test results show that the accelerations increase firstly, and then decrease with the increase of mooring asparagus angle from zero degree to 90 degree;when the asparagus angle is zero degree, the change of acceleration, internal air pressure, water pressure and the change of the asparagus force are significantly larger than the asparagus angle is not zero;The mooring force changes with pulse and is relatively smaller at 45 degree angle;in order to obtain the smaller tension and vibration in towing, the selected mooring asparagus angles of this platform is 45 degree.

  20. Rapid Analysis of Chemical Composition in Asparagus Using Internal Extractive Electrospray Ionization Mass Spectrometry%芦笋中化学成分的内部萃取电喷雾电离质谱检测

    Institute of Scientific and Technical Information of China (English)

    卢海艳; 张华; 彭小明; 陈焕文

    2016-01-01

    选择芦笋(Asparagus Officinalis L)新鲜茎块和两种干燥茎块为代表性植物组织样品,建立了一种直接检测芦笋中多种化学成分的内部萃取电喷雾电离质谱(iEESI-MS)分析方法.在正离子检测模式下,选择甲醇(CH3OH)作萃取溶剂,在无需样品预处理的条件下对芦笋组织样品进行直接质谱分析,获得了组织样品在m/z 50~ 1000范围内的化学指纹谱图,并通过目标离子的碰撞诱导解离(CID)实验,鉴定了对药物和自然产品研发具有重要意义的糖类、氨基酸、生物碱以及芦丁等多种代表性的营养成分.本方法具有无需样品预处理、样品耗量少、操作简便、分析速度快(单个样品的分析时间少于1min)等优点,为食源性植物组织样品的快速分析提供了一种质谱分析新方法.

  1. This kind of 'Asparagus' is quite well liked - Wind energy opinion poll; Die 'Spargel' ist gut angesehen

    Energy Technology Data Exchange (ETDEWEB)

    Gutknecht, B

    2002-07-01

    This article discusses the results of a survey made on the public acceptance of wind energy projects in Switzerland and examines the situation in Switzerland with respect to the use of wind power and its promotion. The results of an internet-based survey made within the framework of a dissertation presented at the Swiss Federal Institute of Technology are presented. This work included 3-D visualisations of proposed wind energy installations at various locations. The results of the survey, which showed a high level of acceptance for the installations, are commented on. The author discusses the ecological considerations relevant to the planning of wind turbines and is of the opinion that there is no danger of wind power installations 'shooting up like asparagus' in the Swiss landscape.

  2. 莴笋叶绿色素的提取与性质研究%Extraction and Properties of Green Pigment From Leaves of Asparagus Lettuce

    Institute of Scientific and Technical Information of China (English)

    鲍霞

    2001-01-01

    Extraction and properties of green pigment from leaves of Asparagus lettuce were reported.Results showed that it was easy to extrad green pigment with 30% acetone and 5% sodium hydroxide,and the powdered pigment possessed good stability against light,heat and sugar.%研究了莴笋叶绿色素的提取及其理化性质。结果表明在30%丙酮及5%氢氧化钠溶液中易于提取,得到绿色粉末状的绿色素,该色素对光、热和糖类具有较好的稳定性。

  3. 攀援天门冬染色体核型分析%Analysis of the Chromosome of Asparagus brachyphyllus Turcz

    Institute of Scientific and Technical Information of China (English)

    赵丽萍; 张韩杰

    2011-01-01

    The number and karyotype of the chromosome of Asparagus brachyphyllus Turcz.were studied.The results show that the chromosome number of the species is 2n=20 and its karyotype formula is K (2n)=20=2M+14m+4sm,belongs to type "1B" of Stebbins' karyotype symmetry.%试验对攀援天门冬体细胞染色体进行了核型分析,结果表明,其染色体数目为2n=20,核型公式为k(2n)=20=-2M+14m+4sm,核型为"IB"型.

  4. 天门冬组培苗生根的影响因素研究%Influencing Factors on Rooting of Tissue Culture Seedling of Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    危革; 欧立军

    2013-01-01

    In order to establish an effective rooting system of tissue culture seedling of Asparagus cochinchinensis,the effects of auxin concentration,sucrose and different basic medium on rooting of tissue culture seedling were studied.The results showed that too high or too low content of nutrition element,auxin and sucrose was not benefit for the rooting of tissue culture seedling.When using 1/2MS with FeSO4·7H2O 6.95 mg/L and Na2·EDTA 9.33 mg/L as the basic medium,supplementing with 0.5 mg/L IBA,0.5 mg/L IAA and 20 g/L sucrose,the rooting effect was the best,with rooting rate as high as 96%.%为了建立有效的天门冬(Asparagus cochinchinensis)组培苗生根系统,分别考察了生长素浓度配比、基本培养基和蔗糖浓度对天门冬组培苗不定根形成的影响.结果表明,培养基中营养元素、激素及蔗糖浓度过高或过低均不利于天门冬组培苗生根.以FeSO4· 7H2O浓度降低到6.95 mg/L、含Na2· EDTA 9.33mg/L的1/2MS为基本培养基,添加0.5 mg/L IBA、0.5 mg/L IAA以及20 g/L蔗糖时生根效果最好,生根率为96%.

  5. 芦笋花药愈伤组织诱导技术研究%Preliminary Study on Anther Culture Callus Inducement of Asparagus

    Institute of Scientific and Technical Information of China (English)

    张元国; 李芳; 包艳存

    2012-01-01

    [目的]对芦笋花药愈伤组织诱导培养技术进行详细研究.[方法]以MS为基本培养基,附加外源激素对二倍体花药愈伤组织诱导技术进行研究.[结果]二倍体芦笋花蕾长度为1.5~2.0 mm时,其诱导效果最好;采用5%高糖浓度可显著抑制花药体细胞分裂和提高花粉愈伤组织诱导率;同一品种不同植株花药愈伤组织诱导率差异显著;适合于二倍体花药愈伤组织诱导的激素配比是1.0 mg/LNAA+ 1.0 mg/L BA.[结论]为芦笋的品种改良与选育奠定了基础.%To study anther culture callus inducement of asparagus in detail. [Method] The diploid anther callus inducement was studied by MS as basic medium with exogenous hormone. [ Result] The length of the diploid bud was 1. 5 - 2. 0 mm which induced result best; The high sugar density of 5% can repress the anther somatic cell division remarkably and improved the inducement rate of pollens callus; The callus inducement rate of the same plant with different species differed greatly; The suitable hormone proportion of diploid anther callus was 1.0 mg/L NAA + 1.0 mg/L BA. [ Conclusion] The study will lay a foundation for improvement and breeding of asparagus.

  6. 影响芦笋产量性状的相关性分析%Relativity Analysis of Influencing the Asparagus (Asparagus officinalis) Yield

    Institute of Scientific and Technical Information of China (English)

    李霞; 刁家连; 张元国; 李书华; 李芳; 杨林

    2005-01-01

    对芦笋的6个农艺学性状指标进行相关性分析,并对产量和一级笋率进行了多重比较.结果表明,影响芦笋产量的重要因子有茎粗、株高和茎数.影响芦笋一级笋率的重要因子有茎粗、株高、产量.植株越高,产量越高,一级笋率也越高.茎粗、株高、茎数构成芦笋产量、品质特性,同时也是新品种选育的必备性状.通过对产量和一级笋率的多重比较发现,全雄品种在产量、一级笋率和其它农艺性状方面明显优于其它常规种,因此芦笋全雄品种的选育已成为目前芦笋育种的重中之重.

  7. 芦笋茎皮化学成分的研究%Studies on the Chemical Constituents of Commmon Asparagus Bark (Asparagus officinalis)

    Institute of Scientific and Technical Information of China (English)

    孙建华; 左春旭; 杨尚军; 仲英; 丁杏苞

    1999-01-01

    从芦笋茎皮中分得7个化合物,经理化常数测定和光谱分析,分别鉴定为:十六烷酸(Ⅰ),二十四烷酸(Ⅱ),β-谷甾醇(Ⅲ),胡萝卜苷(Ⅳ),亚莫皂苷元-3-O-L-鼠李吡喃糖(1→4) β-D-葡萄吡喃糖苷(Ⅴ),asparasaponin Ⅱ(Ⅵ),asparasaponin Ⅰ(Ⅶ).Ⅴ为首次从该属植物中分得的螺旋皂苷.

  8. Genetic diversity of Asparagus (Asparagus officinalis L.)Based on RAPD%芦笋种质资源遗传多样性的RAPD分析

    Institute of Scientific and Technical Information of China (English)

    李霞; 刁家连; 李书华; 杨林; 李芳; 魏秀华; 包艳存

    2005-01-01

    采用RAPD技术对国内外43个芦笋品种的遗传多样性进行分析.从60个随机引物中筛选出12个有效引物,共扩增出183条DNA片段,其中170条为多态性条带,约占总数的92.92%;平均每个引物扩增DNA带数超过15条.结果表明,43份材料的Nei氏相似系数分布在0.407~0.931之间,平均为0.765,可见遗传多样性相对偏低.对所有材料进行聚类分析,在Coefficient=0.77处划等值线,可将参试样品划分为8大类群.其中Jwc1、Purple Passion、鲁芦笋1号等6个品种各单独列为1个类群,其余的被分为2个类群.

  9. High-yield and good quality cultivation techniques for Asparagus (Asparagus officinalis L.)%绿芦笋优质高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    郑绚兰

    2006-01-01

    采用大棚避雨栽培绿芦笋,可有效解决芦笋茎枯病问题,获得优质高产.栽培技术:选择抗性强、色泽浓绿、生长势旺盛的芦笋品种,种子催芽后于育苗床育苗,苗期保持床土湿润;苗高25cm以上、地上茎3根以上、肉质贮藏根4根以上时移栽定植;定植后加强肥水管理,注意预防病虫害;第一年起产的芦笋留2~3根母茎后起产;每年冬季当芦笋茎秆枯黄后应及时清园,并重施腊肥,为翌年优质高产打下基础.

  10. Effects of Different Amount of Nitrogen Fertilizer on the Yield and Harvest Stage of Green Asparagus%不同氮肥用量对绿芦笋产量及采摘期的影响研究

    Institute of Scientific and Technical Information of China (English)

    陈国徽; 高冰可; 熊文; 周庆幈

    2016-01-01

    In this experiment,perennial Asparagus officinalis“JingGang Red”as text material to study the effects of different amount of nitrogen fertilizer on yield and picking period of green asparagus.The results showed that ,the yield of green asparagus increased first and then decreased with the increase of amount of the nitrogen fertilizer dur⁃ing the harvesting time when the amount of nitrogen fertilizer is within N 0~15.4kg/667m2.Applied pure N 11.93kg/667m2 to the perennial asparagus“JingGang Red“the effect is remarkable,the highest yield is 1 321.14kg/667m2.In the process of asparagus harvest,the maximum amount of picking is from late March to early April.%该文以多年生芦笋“井冈红”为试材,研究了不同氮肥用量对绿芦笋产量及采摘期的影响。结果表明,施纯氮在0~15.4kg/667m2范围内,随着氮肥用量的增加绿芦笋产量呈先升高后降低的趋势,其中产量在纯氮用量为11.93kg/667m2时最高,最高产量为1321.14kg/667m2。在芦笋采摘过程中,3月下旬至4月中上旬采摘量最大。

  11. 植酸对芦笋采后贮藏过程中生理变化及酶类的影响%Effect of the Phytic Acid on the Physiological Change and Enzyme of Post-harvest Asparagus

    Institute of Scientific and Technical Information of China (English)

    魏云潇; 何良兴; 谢灵燕

    2011-01-01

    [Objective] The effect of the phytic acid on the physiological change and enzyme of post-harvest asparagus was analyzed. [ Method] The content of chlorophyll, lignin, crude fiber, total soluble sugar and sucrose, and the activity of POD, CAT and PPO of the asparagus, treated with the phytic acid(0. 1% , 0.2% and 0.5% ) , were tested. [ Result] The application of the phytic acid could inhibit the decline in the content of chlorophyll, delay the increment in the content of total soluble sugar, lignin and crude fiber, and promote the formation of sugar. And also, it could intensify the activity of POD, PPO and CAT, which would improve the quality of asparagus through its senescence delaying. [Conclusion] The application of the phytic acid could better improve quality and prolong shift time of asparagus.%[目的]探讨植酸对芦笋采后贮藏过程中生理变化及酶类的影响.[方法]采用植酸3个浓度梯度(0.1%、0.2%、0.5%)处理芦笋,测定叶绿素、纤维素、木质素、可溶性总糖和蔗糖含量,以及POD、CAT、PPO活性的变化.[结果]植酸抑制芦笋叶绿素含量的下降,延缓木质素、粗纤维和可溶性总糖含量的上升,刺激蔗糖含量的升高,有效提高了芦笋CAT、POD和PPO活性,延缓了衰老,保持了绿芦笋的鲜嫩品质.[结论]植酸处理后能较好地保持芦笋的品质,延长保鲜期.

  12. 不同氮肥用量对绿芦笋产量及营养品质的影响%Effects of different amount of nitrogen fertilizer on yield and nutrient quality of green asparagus

    Institute of Scientific and Technical Information of China (English)

    谷永丽; 杨恒山; 刘艳红; 张瑞富; 梁怀宇

    2009-01-01

    2007年3~7月在西辽河平原的内蒙占民族大学实验农场,以3年生芦笋格兰德(Asparagus officinalis L.cv.Grande)为试材,研究了不同氮肥用量对绿芦笋产量及营养品质的影响.结果表明,采笋期施氮量在N 0~360kg/hm2的范围内,绿芦笋产量和合格笋率均随施氮量的增加而增加;嫩茎抽生数和其叶绿素含量的增加是笋产量增加的主要原因.施氮促进细茎笋(直径小于0.5 cm)长粗从而降低了不合格笋的比例.随着氮肥用量的增加,叶绿素a、叶绿素b的含量增加,而类胡萝卜素的含量变化不大.粗蛋白质、可溶性蛋白质、可溶性糖含量均随施氮量的增加而增加;矿质元素含量在适宜施氮水平下较高,高施氮和不施氮处理含量均较低.采笋前期芦丁含量较高且随施氮量的增加而减少,采笋后期芦丁含量降低,且处理间差异变小.在试验条件下,施氮量在N 240 kg/hm2左右,不仅具有较高的产量水平和较好的营养品质,而且增产效果明显.

  13. Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells.

    Science.gov (United States)

    Shirato, Ken; Takanari, Jun; Ogasawara, Junetsu; Sakurai, Takuya; Imaizumi, Kazuhiko; Ohno, Hideki; Kizaki, Takako

    2016-05-01

    Enzyme-treated asparagus extract (ETAS) exerts a wide variety of beneficial biological actions including facilitating anti-cortisol stress and neurological anti-aging responses. However, the anti-skin aging effects of ETAS remain to be elucidated. Reactive oxygen species (ROS) play pivotal roles in skin aging. Increased ROS levels in fibroblasts in response to ultraviolet irradiation activate c-Jun N-terminal kinase (JNK) and its downstream transcription factor activator protein-1 (AP-1), and the resultant gene expression of matrix metalloproteinase (MMP) isoforms accelerates collagen breakdown in the dermis. Therefore, we explored whether ETAS has anti-skin aging effects by attenuating the oxidative stress responses in fibroblasts. Simultaneous treatment of murine skin L929 fibroblasts with hydrogen peroxide (H2O2) and either ETAS or dextrin showed that ETAS significantly suppressed H2O2-induced expression of MMP-9 mRNA as measured by real-time polymerase chain reaction. ETAS also clearly suppressed H2O2-stimulated phosphorylation of c-Jun (AP-1 subunit) and JNK as determined by Western blot. However, ETAS did not affect the increased amounts of carbonyl proteins in response to H2O2, also as determined by Western blotting. These results suggest that ETAS diminishes cellular responsiveness to ROS but does not scavenge ROS. Thus, ETAS has the potential to prevent skin aging through attenuating the oxidative stress responses in dermal fibroblasts.

  14. HPLC/tandem mass spectrometric studies on steroidal saponins: an example of quantitative determination of Shatavarin IV from dietary supplements containing Asparagus racemosus.

    Science.gov (United States)

    Patil, Dada; Gautam, Manish; Gairola, Sunil; Jadhav, Suresh; Patwardhan, Bhushan

    2014-01-01

    Asparagus racemosus (AR) is a popular botanical present in several Ayurvedic medicines and nutritional and dietary supplements with immunomodulatory, galactogogue, and anticancer activity. A steroidal saponin known as shatavarin IV is one of the active constituents of AR. A new, selective, and rapid HPLC/MSIMS method has been developed and validated for quantitative estimation of shatavarin IV in crude, processed, and marketed samples of AR. The analytes were separated on a Luna C18 column using simple isocratic elution with water (0.1% acetic acid)-acetonitrie;(0.1% acetic acid; 70 + 30, vIv) at a flow rate of 0.8 mLlmin. The analytes were detected by electrospray ionization (ESI)-MS/MS and quantified using multiple reaction monitoring techniques in the positive ion mode. The method showed excellent linearity (r2 > 0.998) over the concentration range of 7.5 to 254 ng/mL with LOD of 2.5 ng/mL. Precision (RSD) and accuracy (recovery) were found in the ranges of 2.00 to 5.15 and 102 to 110%, respectively. The validated HPLC/ESI-MS/MS method was successfully applied to the quantification of shatavarin IV in crude, processed, and marketed (single or multiherb) AR samples. Therefore, this method could be used for QC and standardization of pharmaceutical or nutritional products containing AR. PMID:25632427

  15. Enzyme-Treated Asparagus Extract Attenuates Hydrogen Peroxide-Induced Matrix Metalloproteinase-9 Expression in Murine Skin Fibroblast L929 Cells.

    Science.gov (United States)

    Shirato, Ken; Takanari, Jun; Ogasawara, Junetsu; Sakurai, Takuya; Imaizumi, Kazuhiko; Ohno, Hideki; Kizaki, Takako

    2016-05-01

    Enzyme-treated asparagus extract (ETAS) exerts a wide variety of beneficial biological actions including facilitating anti-cortisol stress and neurological anti-aging responses. However, the anti-skin aging effects of ETAS remain to be elucidated. Reactive oxygen species (ROS) play pivotal roles in skin aging. Increased ROS levels in fibroblasts in response to ultraviolet irradiation activate c-Jun N-terminal kinase (JNK) and its downstream transcription factor activator protein-1 (AP-1), and the resultant gene expression of matrix metalloproteinase (MMP) isoforms accelerates collagen breakdown in the dermis. Therefore, we explored whether ETAS has anti-skin aging effects by attenuating the oxidative stress responses in fibroblasts. Simultaneous treatment of murine skin L929 fibroblasts with hydrogen peroxide (H2O2) and either ETAS or dextrin showed that ETAS significantly suppressed H2O2-induced expression of MMP-9 mRNA as measured by real-time polymerase chain reaction. ETAS also clearly suppressed H2O2-stimulated phosphorylation of c-Jun (AP-1 subunit) and JNK as determined by Western blot. However, ETAS did not affect the increased amounts of carbonyl proteins in response to H2O2, also as determined by Western blotting. These results suggest that ETAS diminishes cellular responsiveness to ROS but does not scavenge ROS. Thus, ETAS has the potential to prevent skin aging through attenuating the oxidative stress responses in dermal fibroblasts. PMID:27319149

  16. Use of Isotope Ratio Mass Spectrometry (IRMS) Determination ((18)O/(16)O) to Assess the Local Origin of Fish and Asparagus in Western Switzerland.

    Science.gov (United States)

    Rossier, Joël S; Maury, Valérie; de Voogd, Blaise; Pfammatter, Elmar

    2014-10-01

    Here we present the use of isotope ratio mass spectrometry (IRMS) for the detection of mislabelling of food produced in Switzerland. The system is based on the analysis of the oxygen isotope distribution in water (δ(18)O). Depending on the location on the earth, lake or groundwater has a specific isotopic distribution, which can serve as a fingerprint in order to verify whether a product has grown by means of the corresponding water. This report presents specifically the IRMS technique and the results obtained in the origin detection of fish grown in selected Swiss lakes as well as asparagus grown in Valais ground. Strengths and limitations of the method are presented for both cited products; on one hand, the technique is relatively universal for any product which contains significant water but on the other hand, it necessitates a rather heavy workload to build up a database of water δ(18)O values of products of different origins. This analytical tool is part of the concept of combating fraud currently in use in Switzerland. PMID:25437160

  17. 永安飞桥莴苣上发生的一种新病害为害症状及防治%Symptoms and Control of a New Disease on Feiqiao Asparagus Lettuce in Yong'an

    Institute of Scientific and Technical Information of China (English)

    林积秀; 严叔平; 纪翠红; 黄培枝; 陈文乐

    2013-01-01

      永安市种植茎用莴苣0.67万hm2左右,是当地冬种的支柱产业.近年来,一种未见报道的新病害在当地茎用莴苣上发生为害,植株褪绿心腐死亡,造成田间大量缺株影响种植效益.通过系列的田间调查、筛选、简比试验,目前已基本排除真菌、细菌、病毒、缺素、除草剂药害和种性退化等可能因素,初步确诊是由某一种或某一类虫媒传播的一种新病害,防虫治虫可以有效地减轻其为害.%The cultivating area of Feiqiao asparagus lettuce was about 6 700 hectares in Yong'an city, and asparagus lettuce planting was the pillar industry in Yong'an city in winter. In recent years, a new disease caused serious damage to asparagus lettuce, and the symptoms included leaves chlorisis, stem rot and death, making a large number of dead plants and benefits reduction. Through a series of methods including field investigation, screening test, simple comparison test research, we ruled out the possibility of fungi, bacteria and virus infection, nutrient deficiency, herbicide injury, species degeneration and other factors, and we initially diagnosed the new disease was transmitted by one or a certain kind of insect, moreover the damage of disease could be effectively reduced by controlling insect pests.

  18. 山西省芦笋主要病虫害及无公害生产关键防控措施%Main Asparagus Diseases and Pests and Key Control Methods of Pollution-free Production in Shanxi Province

    Institute of Scientific and Technical Information of China (English)

    史高川; 齐宏立; 张战备; 段国琪; 张惠杰; 耿鹏

    2013-01-01

    芦笋素有“蔬菜之王”的美誉,其作为山西省的一项外向型产业,经济效益良好,但病虫害发生严重,并成逐年扩大趋势.针对山西省芦笋主要病虫害(芦笋茎枯病、枯萎病、根腐病)发病规律以及芦笋木蠹蛾、甜菜夜蛾、斜纹夜蛾的发生规律进行研究,总结出无公害芦笋生产的关键防控措施:主要是一方面加强田间管理、改善现有的管理栽培方法;另一方面以农业、物理、生物防治为主,化学防治为辅,尽量减少化学农药的使用量,控制芦笋病虫害的发生和为害.%Asparagus industry is an important export-oriented industry in Shanxi province with good economic benefit but with pest and disease damage expending year by year. The experiments were conducted to study the regularity of outbreak of asparagus stem blight, blight, purple root rot, cossus, caterpillar, and worm moth and their control measures for pollution—free production. The results showed that, field management should be strengthened and the cultivation methods should be improved. On the other hand, agricultural, physical and biological controls should be mainly taken supplemented by chemical methods but minimizing the chemical pesticides to control asparagus diseases and pests.

  19. Isotherms and kinetic study of dihydrogen and hydrogen phosphate ions (H{2}PO{4}- and HPO{4}2-) adsorption onto crushed plant matter of the semi-arid zones of Morocco: Asphodelus microcarpus, Asparagus albus and Senecio anthophorbium

    Science.gov (United States)

    Chiban, M.; Benhima, H.; Saadi, B.; Nounah, A.; Sinan, F.

    2005-03-01

    In the present work H{2}PO4- and HPO42- ions adsorption onto organic matter (OM) obtained from ground dried three plants growing in arid zones of Morocco has been studied. The adsorption process is affected by various parameters such as contact time, particle size and initial concentration of phosphate solution (Ci ≤ 30 mg/l). The uptake of both ions is increased by increasing the concentration of them selves. The retention of phosphate ions by Asphodelus microcarpus, Asparagus albus are well defined by several isotherms such as the Langmuir, Temkin and Freundlich.

  20. Preservation Effect of Composite Chitosan Coating Treatment on Sea Asparagus%复合壳聚糖涂膜处理对海芦笋保鲜效果的影响

    Institute of Scientific and Technical Information of China (English)

    胡烨; 徐青; 辛建美; 滕宏飞; 罗红宇

    2012-01-01

    Composite chitosan preservative was prepared using chitosan, phytic acid, sodium chloride and orange peel extract according to a certain proportion and storage tests were made at room temperature after sea asparagus immersion and coating. And preservative formula with better preservation effect was selected through orthogonal design. The results showed that each test group of composite chitosan coating could significantly reduce the rot rate and water loss rate of sea asparagus during storage and could effectively delay the oxidation of VC and chlorophyll compared with the control group. The optimized formulation of composite chitosan preservative was chitosan 1.5%, phytic acid 1.5%, sodium chloride 0.2% and orange peel extract 10%. The preservation period of sea asparagus using this formulation could be prolonged for more than 10 days compared with that of the untreated sea asparagus and it can fully meet the requirements of current logistics rate and logistics technology for vegetable preservation period.%壳聚糖、植酸、氯化钠、桔皮提取液按一定比例配制成复合壳、聚糖保鲜剂,在常温下对海芦笋浸泡涂膜后做贮藏试验.采用正交设计筛选保鲜效果较佳的保鲜剂配方.试验结果表明:与空白对照组相比,经复合壳聚糖涂膜处理的各试验组,明显降低海芦笋贮藏期间的腐烂率和失水率,有效延缓VC和叶绿素的氧化.复合壳聚糖涂膜液的优化配方为壳聚糖1.5%,植酸1.5%,氧化钠0.2%,桔度提取液10%(质量分数).经该配方涂膜密封贮藏的海芦笋比未处理的海芦笋保鲜期延长10d以上,可以满足目前物流速率和物流技术对蔬菜保鲜期的要求.

  1. Monitoring of water in soil in asparagus irrigated culture in Vale do Sao Francisco, Pernambuco, Brazil; Monitoracao da agua no solo em cultura irrigada de aspargo no Vale do Sao Francisco, Pernambuco

    Energy Technology Data Exchange (ETDEWEB)

    Antonino, Antonio C. Dantas; Sampaio, Everardo V.S.B.; Dall` Ollio, Attilio; Bernardo, Ana L. Alves [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear; Audry, Pierre [Office de la Recherche Scientifique et Technique d`Outre-Mer (ORSTOM), 75 - Paris (France). Departement Eaux Continentales

    1996-08-01

    For many years the brazilian government has inactivated the implantation of irrigated areas in the Sao Francisco valley, obtaining high productivity.After the most appropriated areas having been occupied, the irrigation of second choice soils, usually more shallower and more clay is been tried. In one of these areas, the productivity of asparagus is less than the expected. trying to improve productivity by optimization of irrigation, the movement of water on soil and plants is being monitored far the last year and a half. the main results are shown with emphasis on the raining season, the most problematic 5 figs.

  2. Reasoned opinion on the modification of the existing MRLs for dithiocarbamates (expressed as carbon disulfide in bulb vegetables, cucurbits and asparagus

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2012-07-01

    Full Text Available

    In accordance with Article 6 of Regulation (EC No 396/2005, Italy, herewith referred to as the evaluating Member State (EMS, received an application from the company BASF Italia Srl. to modify the existing MRLs for dithiocarbamate in cucurbits (edible and inedible peel, onions, shallots, garlic and asparagus, resulting from the use of metiram. In order to accommodate the intended uses, the EMS Italy proposed to raise the existing MRL in garlic from 0.5 mg/kg to 1 mg/kg; for the other crops they considered there was no need to modify the existing EU MRLs. Italy drafted an evaluation report according to Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data were sufficient to derive MRL proposals of 0.6 mg/kg in garlic and 1.5 mg/kg in cucurbits (with inedible peel. For the uses on other crops a need to modify the existing EU MRLs was not identified. Adequate analytical enforcement methods are available to check the compliance of metiram residues (expressed as carbon disulfide and the relevant metabolite ethylenethiourea (ETU in the crops under consideration. EFSA concludes that, the intended use of metiram on garlic and cucurbits (with edible peel will not result in a consumer exposure exceeding the toxicological reference values and therefore is unlikely to pose a public health concern. The exposure situation for the other crops under consideration is not affected by the new uses requested.

  3. The neuroprotective effects and possible mechanism of action of a methanol extract from Asparagus cochinchinensis: In vitro and in vivo studies.

    Science.gov (United States)

    Jalsrai, A; Numakawa, T; Kunugi, H; Dieterich, D C; Becker, A

    2016-05-13

    Extracts of Asparagus cochinchinensis (AC) have antitumor, anti-inflammatory, and immunostimulant effects. The neurobiological mechanisms underlying the effects of AC have not been sufficiently explored. Thus we performed in vivo and in vitro experiments to further characterize potential therapeutic effects and to clarify the underlying mechanisms. In the tail suspension test immobility time was significantly reduced after administration of AC which suggests antidepressant-like activity without effect on body core temperature. Moreover, in animals pretreated with AC infarct size after occlusion of the middle cerebral artery was reduced. In vitro experiments confirmed neuroprotective effects. Total saponin obtained from AC significantly inhibited H2O2-induced cell death in cultured cortical neurons. The survival-promoting effect by AC saponins was partially blocked by inhibitors for extracellular signal-regulated kinase (ErK) and phosphoinositide 3-kinase Akt (PI3K/Akt) cascades, both of which are known as survival-promoting signaling molecules. Furthermore, phosphorylation of Scr homology-2 (SH2) domain-containing phosphatase 2 (Shp-2) was induced by AC, and the protective effect of AC was abolished by NSC87877, an inhibitor for Shp-2, suggesting an involvement of Shp-2 mediated intracellular signaling in AC saponins. Moreover, AC-induced activation of pShp-2 and ErK1/2 were blocked by NSC87877 indicating that activation of these signaling pathways was mediated by the Shp-2 signaling pathway. These effects appear to be associated with activation of the Shp-2, ErK1/2 and Akt signaling pathways. Our results suggest that AC has antidepressant-like and neuroprotective (reducing infarct size) effects and that activation of pShp-2 and pErK1/2 pathways may be involved in the effects. PMID:26947129

  4. Detection of 20 carbamate pesticides in asparagus by ultra performance liquid chromatography-electrospray ionization-mass spectrometry-mass spectrometry%超高效液相色谱-电喷雾串联四极杆质谱法检测芦笋中20种氨基甲酸酯类农药残留

    Institute of Scientific and Technical Information of China (English)

    赵晓琳; 霍乃蕊; 花锦; 宋欢

    2015-01-01

    目的:建立芦笋及其罐头制品中20种氨基甲酸酯类农药残留的定性定量分析方法。方法采用改进的 QuEchERS 法提取和净化,利用超高效液相色谱-电喷雾串联四极杆质谱仪,在多反应监测正离子扫描模式下对样品进行添加回收率试验。结果分别对绿芦笋、白芦笋、绿芦笋罐头、白芦笋罐头4种空白基质添加0.005~0.050 mg/kg农药样品进行回收率试验,回收率为62.44%~85.99%,定量限均为0.005 mg/kg, RSD均小于9%。结论该方法简单快速,灵敏度高,能够同时满足芦笋及其罐头制品中20种氨基甲酸酯类农药残留的检测要求。%Objective To establish an ultra performance liquid chromatography-electrospray ionization-mass spectrometry-mass spectrometry method for detecting of 20 kinds of carbamate pesticides in asparagus and asparagus products. Methods Samples were extracted by modified QuEchERS method, and the mode of multi-reaction monitoring positive ion scanning was applied for analysis by ultra high performance liquid chromatography-electrospray tandem quadrupole mass spectrometer. Results The blank matrixes (green asparagus, white asparagus, canned white asparagus, canned white asparagus) were added 0.005~0.050 mg/kg carbamate pesticides separately and recovery tests were performed. Results demonstrated that the recovery rates were in the range of 62.44%~85.99% and the limits of quantification were 0.005 mg/kg for all the 20 carbamate pesticides tested with the relative standard deviations (RSDs) below 9%. Conclusion The method, not only simple and fast but also high sensitive, can satisfy the international detection requirement for 20 carbamate pesticides residue in asparagus product simultaneously.

  5. Cladistic Evolution Analysis of Asparagus Genus in the Mongolian Plateau%蒙古高原天门冬属植物分支系统演化的研究

    Institute of Scientific and Technical Information of China (English)

    徐杰; 赵一之; 田桂泉

    2003-01-01

    利用最大同步法对蒙古高原天门冬属植物进行了分支分类研究,将本属植物分为2个亚属,天门冬亚属分为2个组.其结果与的分类系统一致.亚属、组的进化顺序为:Subgen. Asparagopsis → Subgen. Asparagus;Sect. densiflorus → Sect. Archiasparagus → Sect. Asparagus.蒙古高原天门冬属植物除Subgen. Asparagopsis中的栽培种A.setaceus和Subgen. Asparagus中Sect. Archiasparagus的A. schoberioides两种外,其余11种均属于Sect. Asparagus的Ser Foliati Iljin.根据分支演化树系图,又可分为Subser. Brachyphyllus和Subser. Persicus.本属的进化趋势为:花两性→花单性;叶状枝具中脉→不具中脉;花梗由短→长;由肉质纺锤状根→绳状根;短粗根状茎→细长根状茎.本文编制了蒙古高原天门冬属植物的分亚属、分组、分种检索表.

  6. 天门冬对D-半乳糖致衰小鼠SOD基因表达影响%The Effect of Asparagus on Expression of Superoxide Dismutase Gene of D - galatose - induced aging Mice

    Institute of Scientific and Technical Information of China (English)

    肖媛媛; 王梓辛; 孙海军; 易大启; 欧立军

    2012-01-01

    采用D-半乳糖致衰小鼠为实验模型,研究天门冬不同提取液对小鼠超氧化物歧化酶(SOD)基因表达的影响.结果发现,天门冬提取液明显提高了衰老小鼠SOD基因的表达,榨汁法、醇提法和水提法的效果依次增加.结果表明,天门冬可以提高SOD基因表达,且水提法最为有效.%The effect of asparagus extracted by different methods on expression of superoxide dismutase (SOD) was studied used Dgalactose induced aging mice as experiment model. The results showed the extracts significantly enhanced the expression of SOD, the expression by water extracts was higher than those by juicer or alcohol extracts. The results indicated that asparagus improved expression of SOD, and the water method was a good extracted methods.

  7. Weeds in Asparagus Plantations in Hainan and Their Control Techniques%海南芦笋园杂草种类调查及杂草防除技术研究

    Institute of Scientific and Technical Information of China (English)

    李晓霞; 黄乔乔; 易克贤; 范志伟; 沈奕德; 程汉亭

    2014-01-01

    A syste matica l investigation of weeds was conducted in asparagus plantation of Hainan Province, and the dominant weed populations were determined. Then, weed control experiments were conducted. The results showed that there were 106 weed species belonging to 23 families in the asparagus garden. Of them, nine species were considered as dominant weeds, including Melinis repens, Panicum maximum, Imperata cylindrica, Digitaria setigera, Chromolaena odorata, Praxelis clematidea, Tridax procumbens and Mimosa pudica. Of the weed control strategies, coverage control and chemical control could control weeds to a certain degree:after 60d covering of nine plants, the control effect reached more than 60%. Covering the weed by cocered Pueraria lobata, Calopogonium mucunoides, Eupatorium odoratum, Mikania micrantha and Tithonia diversifolia increased the shoot number and the diameter of new shoots of asparagus. After application of four kinds of herbicides 60 d, Haloxyfop and acetochlor could control the weeds to a higher extent and were also safe to asparagus plants. In actual production practices, combining the covering and chemical weed control could control weeds to the maximum extent in the asparagus plantation.%系统调查海南芦笋种植区的主要杂草种类,明确芦笋园的杂草优势种群,并以此为基础进行盆栽芦笋化学除草药效试验及覆盖控草试验。结果表明,海南芦笋园常见杂草有21科106种,其中优势种群由马齿苋、红尾翎、短颖马唐、龙爪茅、牛筋草、砖子苗、假臭草、伞房花耳草及鳢肠9种构成。采用覆盖控草和化学除草可在一定程度上控制杂草的发生:覆盖9种植物60 d后,防效可达60%以上,且覆盖葛藤、毛蔓豆、飞机草、薇甘菊、肿柄菊后,芦笋抽笋数和新笋径围都显著高于其余各处理;施用4种除草剂60 d后,盖草能和乙草胺对盆栽芦笋的防效较高且安全。因此,在实际生产中,通过

  8. 利用荧光定量PCR验证与比较豇豆耐旱表达谱%Validation and comparison of drought-responsive microarray with real-time fluorescent quantitative PCR in asparagus bean

    Institute of Scientific and Technical Information of China (English)

    王莎; 徐沛; 汪宝根; 吴晓花; 黄芸萍; 鲁忠富; 刘永华; 李国景

    2013-01-01

    Originating from Africa and domesticated in Asia, asparagus bean [ Vigna unguiculata ssp. sesquipediailis ( L. ) Verdc ] is an important vegetable crop in China. Drought is an important environmental factor restricting the safety of asparagus bean production; therefore it is fundamental to identify drought tolerance genes from the asparagus bean germplasms. Fluorescence quantitative PCR is a useful tool for quantitative assay of gene expression. In this study, we validated and compared expression pattern of a subset of 11 genes from an in-house cowpea cDNA microarray with fluorescence quantitative PCR. It turned out that in roots and leaves of two different asparagus bean cultivars subject to drought stress, qPCR and microarray generally got similar results. The gene/tissue combinations showed congruent expression regulations between the two methods included 27 up-regulated and 7 down-regulated genes, relative to 10 inconsistently observed combinations, giving a rate of consistence of 0. 773. This experiment verified the confidence of high-throughput cDNA microarray in monitoring drought-related gene expressions, which lays a foundation for mining and utilizing drought-tolerant genes in asparagus bean. The causes of differential gene expression patterns between the two methods were discussed.%豇豆[Vigna unguiculata(L.)Walp]起源于非洲,是我国重要的蔬菜作物.干旱是制约长豇豆生产的重要因素,研究和挖掘长豇豆种质中蕴含的耐旱基因是改良长豇豆耐旱性的必要基础.荧光定量PCR是定量检测基因表达的重要手段之一.本实验室前期开发了首张豇豆cDNA表达谱芯片,并利用该芯片研究了长豇豆耐旱表达谱.在此基础上,利用荧光定量PCR对其中11个基因的表达模式进行了验证和比较.试验结果表明,在干旱处理的长豇豆2个品种的根和叶中,供试基因的表达谱芯片分析和qPCR结果一致的共计34组,其中上调27组,下调7

  9. Efeitos de meios de cultura na formação de calos a partir de anteras de aspargo (Asparagus officinalis L. Effects of the culture media on the calli formation from Asparagus officinalis L. anthers

    Directory of Open Access Journals (Sweden)

    Vera Lúcia Bobrowski

    1995-07-01

    Full Text Available Técnicas auxiliares, como a cultura de anteras, possibilitam melhor eficiência na obtenção de novos genótipos de aspargo. Neste trabalho, foram utilizadas anteras contendo grãos de pólen, no estágio uninucleado, dos híbridos 56x22-8 e 47x22-8, em meio MS, semi-sólido e líquido, com diferentes concentrações de reguladores de crescimento: meio A - 0,5 mg/l BAP + 0,1 mg/l ANA + 0,5 mg/l 2,4-D + 20 g/l sacarose + 20 g/l glicose; meio B - 0,5 mg/l 2,4-D + 30 g/l sacarose; meio C - 3,0 mg/l ANA + 0,5 mg/l Kin + 30 g/l sacarose e meio D - 2,0 mg/l ANA + 0,5 mg/l Kin + 30 g/l sacarose. A formação de calos variou com o genótipo e o meio. Os meios líquidos apresentaram melhor eficiência na indução de calos do que os meios semi-sólidos. Para o híbrido 56x22-8 os melhores meios foram o A2 (líquido com 80,5% de indução de calos e dentre os meios semi-sólidos, o meio D1 com 41,6% de calos formados. Para o híbrido 47x22-8, o mais eficiente foi o meio D2 (líquido, com 68,1% de calos formados e, dentre os meios semi-sólidos, o D2, com 15,2%. Não ocorreram diferenças significativas, estatisticamente, entre os meios líquidos nem entre os meios semi-sólidos.Anther culture is an auxiliary technique to obtain new genotypes. In this work, anthers with pollen grains in the uninucleated stage of hybrids 56x22-8 and 47x22-8 were cultured in MS medium, semi-solid and liquid, with different concentrations of growth regulators: medium A - 0,5 mg/l BAP + 0,1 mg/l NAA + 0,5 mg/1 2,4-D + 20 g/l sucrose + 20 g/l glucose; medium B - 0,5 mg/1 2,4-D + 30 g/l sucrose; medium C - 3,0 mg/l NAA + 0,5 mg/l Kin + 30 g/l sucrose and medium D - 2,0 mg/l NAA + 0,5 mg/l Kin + 30 g/l sucrose. Callus formation varied with the genotype and the medium. Liquid media showed more efficiency in callus development than the semi-solids. For the hybrids 56x22-8 the best media were the liquid A2 with 80,5% of callus induction and among the semi-solids the D1, with 41,6%. For the hybrid 47x22-8 were liquid D2 with 68,1% and semi solid D1 with 15,2%. No significant differences were observed among the liquid media nor among the semi-solidones.

  10. Estrategias de presentación de espárragos (Asparagus officinalis L.: preferencias de los consumidores

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2011-01-01

    Full Text Available Los espárragos constituyen un producto hortícola no tradicional altamente perecedero que requiere ser comercializado con una adecuada presentación que permita prolongar su vida útil y al mismo tiempo brindar información sobre el producto como un servicio al consumidor que estimule el consumo. Dentro de las posibilidades de presentación se encuentran los tradicionales atados y actualmente, a nivel global, se está difundiendo el empleo de bandejas que permite una presentación IV Gama del producto final. La IV Gama representa un modelo de producción idóneo para elevar el nivel de servicio de los productos hortícolas frescos en general, lograr una mayor exhibición y la expansión del consumo. Con la finalidad de conocer las preferencias de los consumidores de espárrago respecto de los distintos atributos del producto final, se efectuó un estudio de diez variables: 1 tipos de envases (bandeja: B, atados con y sin film: ACR y ASR; 2 presencia de etiquetas (E; 3 color: en etiquetas (CE; 4 de fondo (CF; 5 de letras (CL; 6 presencia: de recetario (R; 7 de indicaciones de valor nutricional (VN; 8 de ilustraciones (I; 9 de logos (Lg y 10 calibres: G: grande; M: mediano y P: pequeño; asignando a cada una un puntaje de 1 – 10. A tal fin se efectuó un panel test a treinta y cuatro potenciales consumidores el 13/11/2008. Los CF utilizados fueron: 1: verde, 2: amarillo, 3: anaranjado, 4: celeste. Se encontraron diferencias. ACR obtuvo 47 % (puntaje 10, E fue muy valorado (70 % el mayor valor, 85 % consideró importante la presencia de color en las etiquetas, siendo el anaranjado con 53 % el color preferido de fondo, seguido del amarillo (32 % y para las letras el color negro (88 %. R y Lg fue muy valorado por el 90 %, mientras que VN e I por el 80 %. El 61 % de los consumidores prefirió el calibre M.

  11. 芦笋花药离体培养技术研究%Anther Culture of Asparagus officinalis in vitro

    Institute of Scientific and Technical Information of China (English)

    李新江; 建德锋; 陈刚

    2010-01-01

    [目的]提高芦笋的产量.[方法]以不同阶段的芦笋花蕾为外植体,筛选花粉的最佳生长阶段(即最佳材料),研究不同浓度激素配比对芦笋芽分化及生根的影响.[结果]以花粉处于单核期的花蕾为最佳试材,芽分化的最佳培养基为MS+6-BA 2.0 mg/L+NAA 1.0 mg/L;最佳生根培养基为MS+KT 0.1 mg/L+ NAA 0.20 mg/L.[结论]该试验结果为芦笋的快速繁殖奠定了基础.

  12. 芦笋的综合加工利用%The Comprehensive Machining Utilization on Asparagus Officinalis L.

    Institute of Scientific and Technical Information of China (English)

    沈继红; 张爱军

    2005-01-01

    综述了芦笋的营养价值和生理功能,介绍了从植株废弃物中提取芦丁、多糖、菝葜皂甙元、种子油等活性物质的工艺;同时对芦笋种子的营养成分进行了分析评价.

  13. 无公害绿芦笋栽培技术%Pollution-Free Cultivation Technique of Asparagus Officinalis

    Institute of Scientific and Technical Information of China (English)

    俞明全; 郑绚烂; 张文彬

    2006-01-01

    芦笋属百合科多年生宿根性草本植物,嫩茎风味鲜美、营养丰富并有很高的药用价值.浙江省三门县2000年引入种植,2003年组建专业合作社,注册了商标,全面推广无公害生产技术,产品供不应求,效益显著.总结了无公害绿芦笋栽培技术.

  14. Estrategias de presentación de espárragos (Asparagus officinalis L.: preferencias de los consumidores

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2011-06-01

    Full Text Available Los espárragos constituyen un producto hortícola no tradicional altamente perecedero que requiere ser comercializado con una adecuada presentación que permita prolongar su vida útil y al mismo tiempo brindar información sobre el producto como un servicio al consumidor que estimule el consumo. Dentro de las posibilidades de presentación se encuentran los tradicionales atados y actualmente, a nivel global, se está difundiendo el empleo de bandejas que permite una presentación IV Gama del producto final. La IV Gama representa un modelo de producción idóneo para elevar el nivel de servicio de los productos hortícolas frescos en general, lograr una mayor exhibición y la expansión del consumo. Con la finalidad de conocer las preferencias de los consumidores de espárrago respecto de los distintos atributos del producto final, se efectuó un estudio de diez variables: 1 tipos de envases (bandeja: B, atados con y sin film: ACR y ASR; 2 presencia de etiquetas (E; 3 color: en etiquetas (CE; 4 de fondo (CF; 5 de letras (CL; 6 presencia: de recetario (R; 7 de indicaciones de valor nutricional (VN; 8 de ilustraciones (I; 9 de logos (Lg y 10 calibres: G: grande; M: mediano y P: pequeño; asignando a cada una un puntaje de 1 – 10. A tal fin se efectuó un panel test a treinta y cuatro potenciales consumidores el 13/11/2008. Los CF utilizados fueron: 1: verde, 2: amarillo, 3: anaranjado, 4: celeste. Se encontraron diferencias. ACR obtuvo 47 % (puntaje 10, E fue muy valorado (70 % el mayor valor, 85 % consideró importante la presencia de color en las etiquetas, siendo el anaranjado con 53 % el color preferido de fondo, seguido del amarillo (32 % y para las letras el color negro (88 %. R y Lg fue muy valorado por el 90 %, mientras que VN e I por el 80 %. El 61 % de los consumidores prefirió el calibre M.

  15. Effect of Asparagus Instant Powder on Sleep Improvement in Mice%速溶芦笋粉改善小鼠睡眠的研究

    Institute of Scientific and Technical Information of China (English)

    黄云祥; 彭友舜; 王曼; 郑旭静

    2012-01-01

    Objective: To study the effect of Asparagus instant powder (AIP) on sleep in mice. Method; Healthy KM male mice with the body weight of 18-22 g were divided into groups randomly. Then the mice were gavaged with AIP (containing 15% saponins) of low, middle and high doses (85, 170, 510 mg -kg ) ; control group (water) and positive control (Jujuboside 360 mg -kg ) respectively. The treatment was lasted for 30 days, the sleep-producing test was carried out by sub-threshold dose of sodium pentobarbital, and the incubation period test with sodium pentobarbital and the sleep extending test with sodium pentobarbital were also conducted. Result; AIP showed good sleep-producing action in sub-threshold dose test of sodium pentobarbital, could significantly shorten the incubation period induced by sodium pentobarbital, and could significantly prolong sleeping time induced by sodium pentobarbital. Conclusion; AIP can improve the sleep quality.%目的:观察速溶芦笋粉对小鼠睡眠的改善作用.方法:18~22 g健康雄性昆明种小鼠,随机分为5组,每组10只,皂苷含量为15%速溶芦笋粉低、中、高剂量组(85,170,510 mg· kg-1)、空白对照组、阳性对照组(酸枣仁皂苷360 mg·kg-1),连续ig 30 d后,进行戊巴比妥钠阈下剂量催眠试验、戊巴比妥钠睡眠潜伏期试验、延长戊巴比妥钠睡眠时间试验,观察对小鼠睡眠的影响.结果:速溶芦笋粉具有协同戊巴比妥钠阈下剂量催眠作用、可显著缩短戊巴比妥钠睡眠潜伏期、显著延长戊巴比妥钠睡眠时间.结论:速溶芦笋粉具有改善睡眠的作用.

  16. 水溶性壳聚糖涂膜处理对采后绿芦笋贮藏品质的影响%Effect of water-soluble chitosan coatings on postharvest green asparagus quality

    Institute of Scientific and Technical Information of China (English)

    邱苗; 杨成; 蒋珩珺; 陈群超; 黄建颖

    2013-01-01

    研究了0.50%的水溶性壳聚糖(WSC)和羧甲基壳聚糖(CMC)分别对在2℃贮藏条件下采后绿芦笋的涂膜保鲜.通过感官品质、失重率、抗坏血酸含量、叶绿素含量、丙二醛(MDA)含量、总酚含量、多酚氧化酶(PPO)等生理生化指标的测定,以未经处理的绿芦笋为对照.结果表明:经0.50%的CMC和WSC进行涂膜处理,均可有效地降低芦笋的失重率,维持其硬度,延缓其抗坏血酸和叶绿素营养物质的消耗.而且CMC处理可以明显抑制绿芦笋总酚含量的下降及其PPO、POD和SOD活性的变化,其感官品质也明显优于对照组,使绿芦笋的贮藏时间延长至35 d.%The quality of 0.50% water-soluble chitosan (WSC) and carboxymethyl chitosan (CMC) coatings on postharvest green asparagus which were stored at 2℃ was investigated.Base on the data of sensory evaluation, weight loss percentage, the contents of ascorbic acid, chlorophyll, malonaldehyde ( MDA) , total phenolic and polyphenolox-idase (PPO) , peroxidase (POD) , super oxygen dehydrogenises (SOD) activity testing, both 0.50% WSC and CMC treatments could dramatically retard the decrease of weight loss, firmness, the loss of chlorophyll and ascorbic acid during the storage.Moreover, 0.50% CMC treatment not only could keep the total phenolics content, but restrain the changes of PPO, POD, and SOD activity, presenting better quality of asparagus than the control during the cold storage , and prolong the shelf life of postharvest green asparagus to 35 days.

  17. CRECIMIENTO, ABSORCIÓN DE FÓSFORO Y MORFOLOGÍA DE LA RAÍZ EN ESPÁRRAGOS INOCULADOS CON HONGOS MICORRIZALES Y PSEUDOMONAS FLUORESCENTES GROWTH, PHOSPHORUS ABSORPTION AND ROOT MORPHOLOGY OF ASPARAGUS INOCCULATED WITH MYCORRHIZAL FUNGI AND FLUORESCENT PSEUDOMONAS

    Directory of Open Access Journals (Sweden)

    Juan Carlos Pérez Naranjo

    2004-12-01

    Full Text Available En un experimento bajo invernadero se evaluaron los efectos de la aplicación de fósforo (P y la inoculación con Glomus fistulosum y Pseudomonas aeruginosa sobre el crecimiento, la longitud de la raíz y la absorción de P por plantas de espárrago sembradas en un Alic Melanudand. La inoculación con G. fistulosum incrementó significativamente la masa seca de la parte aérea y de raíces, la longitud de raíces primarias y secundarias y la absorción de P por las plantas. La aplicación de P aumentó significativamente el P disponible en el suelo (Bray II; sin embargo, a pesar de tal incremento no hubo aumento significativo del crecimiento de las plantas. La inoculación con P. aeruginosa no tuvo efecto significativo sobre las variables estudiadas, ni se encontraron interacciones significativas entre los factores.A greenhouse experiment evaluated the effects of phosphorus (P application and inoculation with Glomus fistulosum and Pseudomonas aeruginosa on growth, root length, and P uptake of Asparagus officinalis grown on Alic Melanudand. Inoculation with G. fistulosum significantly increased shoot and root dry weight, primary and secondary root lengths, and plant P uptake. Phosphorus application significantly increased extractable P in the soil (Bray II, but despite this increase, there was no significant increase in plant growth. Inoculation with P. aeruginosa had no significant effect on the variables studied, nor were there significant interactions among the factors.

  18. 28个绿芦笋品种的适应性评价%Evaluation of the Adaptability of 28 Green Asparagus Cultivars

    Institute of Scientific and Technical Information of China (English)

    祝清俊; 陈蕾蕾; 孙欣; 张奇志; Mikolaj Knaflewski; Wodzimierz Krzesi(n)ski

    2013-01-01

    [目的]本文旨在对波兰气候条件下的28个绿芦笋品种进行适应性评价,并选择出最适合在波兰种植的品种.[方法]2008年在马赛林试验站对第六年收获的28个品种的绿芦笋进行评价.收获期间,将收获的芦笋嫩茎,剪至22 cm,分级,称重,同时对嫩茎外观、花青素变化、芦笋头紧实性、颜色及光滑度等性状进行评估.[结果]‘Gynlim’是产量最高的品种,其次为‘Hannibal’,‘Ravel’,‘Grolim’和‘Ramada’.‘Gloria’是最差的品种,其它最差的品种以次为‘Tsuki-kho 3’、‘Purple Passion’、‘Jacq.Ma 2014’、‘Sartaguda’、和‘Jacq.Ma2004’.‘Gynlim’、‘Hannibal’、‘Ravel’、‘Ramada’、‘Ariane’是前期产量最高的前5个品种.前期产量最差的品种为‘PLA-2132’,随后是‘Ramos’、‘Tsuki-kho 3’和‘Purple Passion’.‘Ariane’、‘Cipress’、‘Grolim’、‘Gynlim’和‘Hannibal’的嫩茎质量最好.嫩茎质量最差的品种依次是‘Jacq.Ma 2004’、‘Gloria’、‘Jacq.Ma 2014’和‘Tsuki-kho 3’.[结论]综合考虑产量与质量,在波兰气候条件下,最适宜的绿芦笋品种为‘Gynlim’、‘Hannibal’、‘Ravel’和‘Grolim’,而‘Gloria’、‘Tsuki-kho 3’、‘Purple Passion’、‘Jacq.Ma 2014’和‘Sartaguda’不适合波兰种植.%[Objective] This study aimed to evaluate the adaptability of 28 green asparagus cultivars under the climatic conditions in Poland and to select cultivars most suitable for growing in Poland.[Method] Based on an overview of asparagus,spears of 28 cultivars from the six year's harvest in 2008 were evaluated at the Marcelin Experimental Station.During the harvest period,spears were cut daily and trimmed to 22 cm,followed by grading and weigh.In addition,general appearance,anthocyan discolouration,head tightness,colour and smoothness of the spears were also evaluated.[Result] In terms of total yield,‘Gynlim’ was the best

  19. The extraction of asparagus polysaccharide and its effect on the growth of human hepatic cancer SMMC-7721 cells in vitro%天冬多糖的提取及其对人肝癌SMMC-7721细胞生长影响的研究

    Institute of Scientific and Technical Information of China (English)

    张闽光; 陈刚; 刘力

    2011-01-01

    Objective To investigate the technical reliability of extracting polysaccharide from asparagus cochinchinensis and to discuss the effect of asparagus polysaccharide on the growth of a human hepatic carcinoma cell line. SMMC-7721 cells. in vitro. Methods The asparagus cochinchinensis was soaked in cold water and the crude asparagus polysaccharide was extracted with ethanol precipitation. Then,the crude asparagus polysaccharide was deproteinized with the method of pronase E and Sevag. Finally , the deproteinized asparagus polysaccharide was purified with the cellulose and sepharose chromatographic column, which was further identified and its molecular weight was determined. The effect of asparagus polysaccharide in different dose on the growth of the hepatic carcinoma SMMC-7721 cells was estimated with MTT assay in vitro, and the dose response curve as well as the growth curve was drawn. Results The extracted substance obtained by authors' method was further purified and deproteinized, and asparagus polysaccharide was thus gained. Its molecular weight ranged from 5 000 to 400 000 u. It had a twofold regulative effect on the growth of the hepatic carcinoma SMMC-7721 cells. When its concentration was lower,below 800 μg/ml, the asparagus polysaccharide had the effect of promoting the vegetation of the hepatic carcinoma SMMC-7721 cells. However. when its concentration was higher, over 900 μg/ml, the asparagus polysaccharide showed certain inhihitory effect on the vegetation of SMMC-7721 cells. besides,with the time passing by and the concentration increasing, the inhibitory effect became more effective ( P < 0.05 ). Conclusion The results of this experiment indicate that authors' method to extract, separate and purify the asparagus polysaccharide is technically reliable. In vitro, low concentration of asparagus polysaccharide can promote, while high concentration of asparagus polysaccharide can suppress, the vegetation of the SMMC-7721 cells, and the effectiveness is

  20. 芦笋老茎堆肥中嗜热放线菌的分离和鉴定%Isolation and Identification of Thermophilic Actinomycetes in Asparagus Old Stem Compost

    Institute of Scientific and Technical Information of China (English)

    陈婷婷; 王丽芳; 王琪; 韩建荣

    2013-01-01

    采用稀释涂布法对芦笋老茎堆肥不同发酵阶段6个样品中的嗜热可培养放线菌进行分离,经纯化得到菌落形态有明显区别的23个菌株;根据16S rDNA序列分析结果,其中,4株的序列与白浅灰链霉菌Streptomyces albogriseolus的同源性最高,5株的序列与热普通链霉菌Streptomyces thermovulgaris的同源性最高,2株的序列与假浅灰链霉菌Streptomyces pseudogriseolus的同源性最高,1株的序列与刺棘链霉菌Streptomyces espinosus的同源性最高,有11株在GenBank数据库中未找到与其相似的已知链霉菌种的序列,分类地位待定.结果说明,芦笋老茎堆肥中优势的嗜热可培养放线菌主要是白浅灰链霉菌和热普通链霉菌.%The culturable thermophilic actinomycetes in the asparagus old stem compost samples at different fermentation stages were investigated. By dilution-plate method, twenty-three strains of actinomycetes were isolated from the samples. According to 16S rDNA sequence analysis, preliminary identification of these actinomycetes strains was done. Among these 23 stains of actinomycetes, four strains showed the highest similarity to Streptomyces albogriseolus, five strains to Streptomyces thermovulgaris, two strains to Streptomyces pseudogriseolus, and one strain to Streptomyces espinosus. Eleven strains could not be identified because there were no matched gene sequences of Streptomyces spp. To be found in the GenBank. The results indicated that Streptomyces albogriseolus and Streptomyces thermovulgaris were the most frequently isolated thermophilic actinomycetes from the asparagus old stem compost.

  1. Spatial Pattern of Infected Plants and Sampling Methods of Asparagus (Asparagus officinalis) Stem Blight%芦笋茎枯病病株空间分布型及其抽样研究

    Institute of Scientific and Technical Information of China (English)

    王向阳

    2004-01-01

    对芦笋茎枯病病株空间分布型及其抽样技术进行研究.结果表明,芦笋茎枯病病株在新栽芦笋田多为随机分布,在栽植3年以上的芦笋田以聚集分布为主,分布的基本成分是个体群,聚集强度的大小与发病轻重无关.造成多种分布的原因主要与病害本身的侵染和传播特性有关.抽样方法中,以棋盘式抽样最佳,其次是对角线式.同时给出了不同病株率下的理论抽样数.

  2. Effects of application of "Youji lüzhibao" on Asparagus (Asparagus officinalis L.)%有机绿之宝在芦笋上的应用效果研究

    Institute of Scientific and Technical Information of China (English)

    陈云平; 陈福如; 郑宝团; 欧金顺; 林增谋; 陈玉秀; 林美娟

    2006-01-01

    以12.5%有机绿之宝对春季萌芽前芦笋实施灌根试验,以探讨有机绿之宝在芦笋上的应用效果.结果表明:12.5%有机绿之宝能显著提高芦笋的产量和一级笋率,其中以2000倍液灌根效果最好,增产幅度达22.76%,一级笋率提高10.78%.与对照(清水)相比,差异达显著水平.

  3. Effects of Glutathione on the Lignification and Quality of Postharvest Green Asparagus (Asparagus officinalis L.)%谷胱甘肽对采后石刁柏木质化和食用品质的影响

    Institute of Scientific and Technical Information of China (English)

    刘尊英; 姜微波

    2005-01-01

    在(24±1)℃条件下,谷胱甘肽(GSH)可显著抑制采后石刁柏木质素合成前体总酚的含量及与木质素合成相关的苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活性上升,延缓叶绿素、可溶性糖、可溶性蛋白和核酸的降解,降低活性氧和木质素含量,从而保持石刁柏的鲜嫩品质.

  4. Isolation, Screening and Identification of Thermophilic Cellulolytic Bacteria in Old Stalk of Asparagus Compost%芦笋老茎堆肥中高温纤维素分解菌的分离、筛选及鉴定

    Institute of Scientific and Technical Information of China (English)

    李欣欣; 王琳琳; 申挺挺; 宋志刚; 韩建荣

    2012-01-01

    采用纤维素刚果红培养基在45℃下从芦笋老茎堆肥中分离筛选出52株高温纤维素分解菌.通过水解圈直径与菌落直径比值的比较,进一步获得了6株纤维素分解能力较强的菌株.经菌落形态观察、革兰氏染色、芽孢染色和生理生化试验,6株高温纤维素分解菌初步鉴定为需氧芽孢杆菌(Bacillus spp.).%In this experiment, 52 strains of thermophilic cellulolytic bacteria were isolated and screened from the old stalk of asparagus compost using cellulose - congo red culture medium at 451. By comparing the ratio of hydrolytic circle diameter to colony diameter, 6 strains of cellulolytic bacteria with higher efficient were selected, and they were identified as Bacillus spp. through morphological observation, Gram' s staining, spore staining and physiological - biochemical experiments.

  5. 羊齿天门冬根茎提取物对人骨肉瘤细胞增殖的抑制作用%Inhibitory effect of extracts from asparagus filicinus rhizome on proliferation of human osteosarcoma cells

    Institute of Scientific and Technical Information of China (English)

    瞿家权; 石莺; 贾薇; 张永东; 钟飞

    2014-01-01

    目的:探讨羊齿天门冬根茎提取物对人骨肉瘤细胞增殖的影响及其分子机制。方法 MTT法检测羊齿天门冬根茎提取物对人骨肉瘤Saos-2细胞增殖和生长的影响;平皿集落形成法检测羊齿天门冬根茎提取物对细胞锚定依赖性生长的影响;碘化丙啶单染流式细胞仪检测细胞周期分布改变;Western blot检测环氧化酶2(COX-2)蛋白表达水平改变。结果羊齿天门冬根茎乙酸乙酯部位(AF-A)对Saos-2细胞具有细胞毒作用(IC50值为26.7μg/mL);AF-A对Saos-2细胞锚定依赖性生长具有抑制作用,呈剂量相关性(P<0.05);AF-A(30.0、100.0μg/mL)处理Saos-2细胞48 h后,细胞S期百分比分别为(43.7±2.5)%和(51.9±1.9)%,与对照组(31.8±4.8)%比较差异有统计学意义(P<0.05);不同浓度AF-A均能抑制Saos-2细胞COX-2蛋白水平表达。结论 AF-A对人骨肉瘤细胞增殖和生长具有抑制作用,其机制可能与诱导人骨肉瘤细胞S期阻滞和抑制COX-2蛋白水平表达有关。%Objective To investigate the inhibitory effect of extracts from asparagus filicinus rhizome on prolifieration of human osteosarcoma Saos-2 cells and its molecular mechanism .Methods MTT assay was used to detect the cytotoxic activity and growth inhibition of three different extracts from asparagus filicinus rhizome against Saos-2 cells ;plate colony formation assay was per-formed to detect active fraction of asparagus filicinus rhizome on the anchorage dependent growth of Saos-2 cells ;the cell cycle alter-ation was determined by propidium iodide staining and flow cytometry analysis ;the alteration of protein expression level of COX-2 was determined by using Western blotting .Results Ethyl acetate fraction of asparagus filicinus rhizome (AF-A) exerted the potent cytotoxicity on Saos-2 cells(IC50 =26 .7 μg/mL);AF-A induced the inhibitory effect on the anchorage dependent growth

  6. Evaluation of Biochemical Contents, Trace Elements, Nutritive Value and HPTLC Profiling in Two Edible Food Plants Based Diets

    Directory of Open Access Journals (Sweden)

    Ali Aberoumand

    2014-09-01

    Conclusions: Our findings suggest that both Asparagus officinalis DC and Chlorophytum comosum Linn are endowed with antioxidant phytochemicals and nutritive values. Keywords: Asparagus officinalis DCChlorophytum comosum Linn, Nutritive values

  7. Manejo de irrigação para o feijão-de-metro, nas fases vegetativa e produtiva, em ambiente protegido Irrigation scheduling for asparagus bean in vegetative and productive stages in greenhouse

    Directory of Open Access Journals (Sweden)

    Wellington G. da Silva

    2012-09-01

    Full Text Available O manejo adequado da irrigação é uma prática agrícola que requer informações para cada cultura explorada, em particular nas fases fenológicas do ciclo produtivo pois muitas espécies possuem períodos críticos durante os quais o estresse hídrico causa sérias reduções na produção. Este trabalho teve, como objetivo, avaliar o efeito de cinco níveis de potencial matricial de água no solo sobre o crescimento e produção do feijão-de-metro. O experimento foi conduzido em casa de vegetação, na área experimental pertencente ao Departamento de Engenharia da Universidade Federal de Lavras, Lavras, MG. O delineamento experimental foi o de blocos casualizados, em esquema fatorial 5 x 2, com quatro repetições. Os tratamentos foram constituídos de cinco potenciais matriciais de água no solo como indicativos do momento de irrigar (-15, -35, -55, -75 e -95 kPa, em duas fases fenológicas (vegetativa e produtiva, monitorados por sensores instalados a 0,15 m de profundidade. Os resultados permitiram concluir que a fase mais sensível ao déficit hídrico foi a produtiva e que a irrigação realizada no potencial matricial em torno de -15 kPa induziu a melhor resposta da cultura quanto ao desenvolvimento, produção e qualidade das vagens.Proper irrigation water management requires knowledge on each exploited crop; particularly regarding vegetative and productive phases since many species have critical periods during which water stress causes serious yield reductions. This study was undertaken so as to assess the effect of five matric potential levels on asparagus bean growth and yield. The experiment was conducted under greenhouse conditions at the experimental area of Department of Engineering, Federal University of Lavras, Lavras, MG. The experimental design was randomized blocks in factorial 5 x 2. The treatments consisted of five matric potentials as indicative of the irrigation scheduling -15 -35 -55 -75 -95 kPa and at two

  8. 15N Isotope Techniques for Estimating Effects of Urea-N Fertilizer Application Rate on Yieids and Nutrient Contents of Pakchoi Cabbage and Asparagus Lettuce and Nitrogen Utilization Efficiency

    Institute of Scientific and Technical Information of China (English)

    LIAO Yu-Iin; ZHENG Sheng-xian; RONG Xiang-min; LIU Qiang; FAN Mei-rong

    2010-01-01

    A pot experiment combined with15 N isotope techniques was conducted to evaluate effects of the varying rates of urea.N fertilizer application on yields,quailty,and nitrogen use efficiency(NUE)of pakchoi cabbage(Brassica chinensis L.)and asparagus lettuce(Lactuca saiva L.).15 N-labbled urea(5.35 15 N atom%)was added to pots with 6.5kg soil of 0.14,0.18,0.21,0.25,and 0.29 g N/kg soil.and applied in two splits:60 percenl as basel dressing in the mixture and 40 percent as toodressing.The fresh yields of two vegetable species increased with the increasing input of urea-N,but there was a significant quadratic relationship between the dose of urea-N fertilizer application and the fresh yields.Whan the dosage of urea-N fertilizer reached a certain value,nitrate readily accumulated in the two kinds of plants due to the decrease in NR activity;furthermore,there was a linear nagative correlation between nitrate content and NR activity.With the increasing input of urea-N.ascorbic acid and soluble sugar initially increased,declined after a while,and crude fiber rapidly decreased too.Total absorbed N(TAN).N derived from fertilizer(Ndff),and N derived from soil(Ndfs)increased,and the ratio of Ndff and TAN also increased.but the ratio of Ndfs and TAN as well as NUE of urea-N fertilizer decreased with the increasing input of urea-N.These results suggested that the increasing application of labeled N fertilizer led lo the increase in unlabeled N(namely,Ndfs)presumably due to"added nitrogen interaction"(ANI),the decease in NUE of urea-N fertilizer may be due to excess fertilization beyond the levels of plant requirements and the ANI.and the decrease jn the two vege table yields with the increasing addition of urea-N possibly because the excess accumulation of nitrate reached a toxic level.

  9. Analysis of Onion and Asparagus Agrochemical Usage in America in 2010%2010年美国洋葱、芦笋用药情况调查与分析

    Institute of Scientific and Technical Information of China (English)

    崔丽; 黄文九; 张佳; 袁会珠

    2012-01-01

    美国农业部农业统计中心(National Agricultural Statistics Service,NASS)日前公布了2010年度蔬菜中农药使用情况的统计报告。此报告中的数据采自2010年9月—2011年1月,对19个州的样本进行了统计,考察对象包括29种蔬菜作物。本文依据美国农业部发布的报告,对美国2010年茎菜类蔬菜洋葱、芦笋上常用杀虫剂、杀菌剂和除草剂的使用情况作了详细的计算和分析。按照有效成分统计,洋葱上使用杀虫剂有36种、杀菌剂37种、除草剂40种,其中使用最普遍的分别是灭多威、代森锰锌和乙氧氟草醚。芦笋上使用的杀虫剂有15种、杀菌剂5种、除草剂28种,其中使用最普遍的分别是甲萘威、百菌清和敌草隆。调查数据分析表明,美国在洋葱、芦笋等茎菜类蔬菜生产中使用的农药品种以传统品种为主。%NASS list the vegetable chemical usage survey recently. The survey was conducted on 29 vegetable crops in 19 states. The data collection occurred during the months of September 2010 through January 2011. According to the report, we made a detailed caculation and analy- sis of the commonly used insecticides, fungicides and herbicides on two stem vegetables, as- paragus and onion. In accordance with active ingredient, 15 kinds of insecticides, 5 kinds of fungicides and 28 kinds of herbicides were used in asparagus, and the most commonly used in- secticide, fungicides and herbicides were carbaryl, chlorothalonil and diuron respectively. There are 36 kinds insecticides, 37 kinds fungicides and 40 kinds herbicides used on onion, and the most commonly used insecticide, fungicides and herbicides were methomyl, mancozeb and oxyfluorfen. The data analysis demonstrated that American' s pesticides usage in the pro- duction of stem vegetables were the traditional varieties.

  10. Alternativas para enfrentar las actuales exigencias del mercado de espárrago (Asparagus officinalis L. verde en Argentina. 2. Tendencias de la demanda

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2012-01-01

    Full Text Available El espárrago constituye una hortaliza muy valorada a nivel global, cuya difusión en Argentina es limitada, por lo cual se requiere de estrategias para fomentar su consumo. A tal fin se realizó una encuesta (10/11/2009 en Azul, Argentina, estudiando: preferencias acerca del consumo, beneficios de su consumo (B, calibre (C y longitud (L de turiones, tipo de envase (E, conocimiento sobre vida útil de las presentaciones (TVU, presencia de etiquetas (PE y características como color de fondo (CF y de letras (CL, lugar de compra (LC, tiempo (Ti desde la compra hasta el consumo y prioridad (P para la compra (calidad o precio, entre otros ítems. Los encuestados manifestaron preferencias de consumo de espárragos verdes y en platos calientes, mostrando desinterés por el consumo de espárragos enlatados. En B, > 95 % demostró conocer las propiedades nutricionales y saludables de los espárragos. 71 % mostró interés por calibre mediano y un 75 % cortos (15-20 cm. Respecto de E, prefirieron las presentaciones en bandejas y atados con film, y con presencia de etiquetas, color de fondo llamativo (naranja y amarillo y letra negra. El 81,1 % adquieren espárragos mediante venta directa y 50 % manifestó que transcurre entre 2 días y 1 semana entre la adquisición y el consumo, prefiriéndolos frescos y no congelados. En P, el 90 % priorizó calidad en lugar del precio. Estos resultados indican que los consumidores no son indiferentes a los atributos de presentación del producto, por lo cual los empresarios deberían esforzarse por mejorar las dimensiones de la calidad del producto, por lo que se verían recompensados.

  11. Alternativas para enfrentar las actuales exigencias del mercado de espárrago (Asparagus officinalis L. verde en Argentina. 1. Empleo de híbridos

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2012-01-01

    Full Text Available A fin de determinar la productividad de híbridos de espárrago verde: 7 de origen italiano y un testigo americano (UC-157, en su segunda cosecha, se realizó un ensayo el 22/11/2006, con dos tamaños de plantines (PG y PP. Se determinó: producción fresca total (PFT y por cosecha (PFTC, producción comercial (PFN y por cosecha (PFNC, número de turiones totales (NTT, comerciales (NT, comerciales por cosecha (NTC y por planta (NTP, peso promedio por turión (PPT y distribución de calibres. Para todas las variables se destacó PG. En promedio, PFT fue de 13.287 kg/ha, PFN: 5.791 kg/ha, mientras que NT: 452.479 turiones/ha, lográndose una productividad por planta de 558 g totales y 243 g netos, y NTP de 19 turiones/planta. El PPT fue 12,95 g. Respecto de los híbridos, en PFT se destacó UC-157, seguido de Zeno, Eros, Ercole y H668 kg/ha (p < 0,05; mientras que en PFNC: UC-157 286,1; seguido por Giove 231,4; Zeno 230,7; Ercole 227,9 y Eros 224,3 kg/cosecha (p < 0,05. En NT, UC-157 produjo 186.646 turiones/ha comerciales más, respecto al promedio obtenido del conjunto de genotipos italianos, siendo el que menos turiones produjo Giove: 361.179 turiones/ha. En NTC, se destacó UC-157, seguido por Zeno y Ercole (p < 0,05. Logró mayor PPT Giove (16,7, seguido de Eros (13,1 y H668 (12,8 g/turión. En calibres el 43 % correspondió a L. Los genotipos italianos presentaron turiones de mayores calibres y menor defecto de espigado.

  12. Chemical Components in Asparagus officinalis and Their Medical Effects%芦笋的化学成分及其药理作用

    Institute of Scientific and Technical Information of China (English)

    鞠玉栋

    2011-01-01

    芦笋是一种质地优良、营养丰富的保健型食药兼用名贵蔬菜,介绍芦笋中含有的多种化学成分和活性成分,并着重对芦笋的抗癌、提高免疫力、降血脂、抗表老等药理作用进行综述.

  13. DETERMINATION OF TOTAL FLAVONOID IN ASPARAGUS OFFICINALIS LINN BY SPECTROPHOTOMETRY%分光光度法测定芦笋中总黄酮的含量

    Institute of Scientific and Technical Information of China (English)

    宋元清; 王艳平; 毛远菁

    2005-01-01

    黄酮类化合物与铝盐形成配合物,该配合物在紫外可见光区有吸收,选择510 nm为测定波长,芦丁作为对照品,用分光光度法测定芦笋中的总黄酮,平均回收率为97.23%,测定结果的相对标准偏差为2.13%(n=5).

  14. Determination of Selenium in Asparagus officinalis Linn%芦笋中硒元素的测定方法探究

    Institute of Scientific and Technical Information of China (English)

    于辉; 吕瑞芳; 秦博文; 陈超

    2013-01-01

    目的:建立测定芦笋中硒含量的方法.方法:采用硝酸-高氯酸微波消解后,在铁氰化钾-盐酸体系中,选用最佳氢化物发生原子荧光法测定条件,采用标准曲线法定量分析.结果:该法线性范围为0-0.8 μg/mL,检出限为0.024 μg/mL,回收率为96.7-103.3%,相对标准偏差为1.28%.结论:本方法简单、准确,适用于芦笋中硒的含量测定.

  15. Inhibitory Effects of Juice from Asparagus Officinalis L. on Cyclophosphamide(CTX)-induced Mutagenic Activities in Mice%芦笋汁的抗突变作用

    Institute of Scientific and Technical Information of China (English)

    汤新慧; 高静

    2001-01-01

    用微核(MN)试验、姐妹染色单体交换(SCE)及精子畸形试验探讨芦笋汁对环磷酰胺(CTX)致突变作用的影响.结果表明,单纯芦笋汁对小鼠骨髓细胞MN和SCE及精子畸形均无明显影响,但一定剂量芦笋汁可使CTX诱发的MN率(6.00%)降低(5.00%~2.66%),SCE(29.22次/细胞)减少(26.40~13.37次/细胞),精子畸形率(6.10%)下降(5.12%~2.86%),均呈现剂量-效应关系.

  16. Alternativas para enfrentar las actuales exigencias del mercado de espárrago (Asparagus officinalis L. verde en Argentina. 1. Empleo de híbridos

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2012-12-01

    Full Text Available A fin de determinar la productividad de híbridos de espárrago verde: 7 de origen italiano y un testigo americano (UC-157, en su segunda cosecha, se realizó un ensayo el 22/11/2006, con dos tamaños de plantines (PG y PP. Se determinó: producción fresca total (PFT y por cosecha (PFTC, producción comercial (PFN y por cosecha (PFNC, número de turiones totales (NTT, comerciales (NT, comerciales por cosecha (NTC y por planta (NTP, peso promedio por turión (PPT y distribución de calibres. Para todas las variables se destacó PG. En promedio, PFT fue de 13.287 kg/ha, PFN: 5.791 kg/ha, mientras que NT: 452.479 turiones/ha, lográndose una productividad por planta de 558 g totales y 243 g netos, y NTP de 19 turiones/planta. El PPT fue 12,95 g. Respecto de los híbridos, en PFT se destacó UC-157, seguido de Zeno, Eros, Ercole y H668 kg/ha (p < 0,05; mientras que en PFNC: UC-157 286,1; seguido por Giove 231,4; Zeno 230,7; Ercole 227,9 y Eros 224,3 kg/cosecha (p < 0,05. En NT, UC-157 produjo 186.646 turiones/ha comerciales más, respecto al promedio obtenido del conjunto de genotipos italianos, siendo el que menos turiones produjo Giove: 361.179 turiones/ha. En NTC, se destacó UC-157, seguido por Zeno y Ercole (p < 0,05. Logró mayor PPT Giove (16,7, seguido de Eros (13,1 y H668 (12,8 g/turión. En calibres el 43 % correspondió a L. Los genotipos italianos presentaron turiones de mayores calibres y menor defecto de espigado.

  17. 耐盐芦笋的组培快繁技术研究%Research of Quick Cultivate Technology on Salt-torlerent Asparagus Officinalis

    Institute of Scientific and Technical Information of China (English)

    徐化凌; 陈纪香

    2009-01-01

    东营市农业科学研究所立足于黄河三角洲实情,以新萌发的芦笋嫩茎为试材,研究不同激素组合对诱导芦笋茎段愈伤组织的影响、对细胞胚的影响试验及对细胞胚的优化诱导试验,培育出2个耐盐能力强、产量高、品质优,适合广大盐碱地区种植的耐盐芦笋系号,开发前景极好.

  18. STUDY ON 1-MCP AND LOW TEMPERATURE STORAGE OF ASPARAGUS OFFICINALIS L.%1-甲基环丙烯和低温保鲜芦笋研究

    Institute of Scientific and Technical Information of China (English)

    苏光耀; 王向阳

    2006-01-01

    研究了1-甲基环丙烯(1-MCP)和低温对采后贮藏中芦笋品质的影响,测定了芦笋的维生素C含量、叶绿素含量、剪切力和电导率,并进行了感官评价.结果表明,2℃贮藏条件和1-MCP处理方法结合可以有效地减少芦笋的维生素C和叶绿素的损失,维持较理想的剪切力,其电导率比较低,具有比较好的感官品质.说明其能延缓芦笋的衰老,从而延长芦笋的贮藏保鲜期.

  19. Alternativas para enfrentar las actuales exigencias del mercado de espárrago (Asparagus officinalis L. verde en Argentina. 2. Tendencias de la demanda

    Directory of Open Access Journals (Sweden)

    Ana M. Castagnino

    2012-12-01

    Full Text Available El espárrago constituye una hortaliza muy valorada a nivel global, cuya difusión en Argentina es limitada, por lo cual se requiere de estrategias para fomentar su consumo. A tal fin se realizó una encuesta (10/11/2009 en Azul, Argentina, estudiando: preferencias acerca del consumo, beneficios de su consumo (B, calibre (C y longitud (L de turiones, tipo de envase (E, conocimiento sobre vida útil de las presentaciones (TVU, presencia de etiquetas (PE y características como color de fondo (CF y de letras (CL, lugar de compra (LC, tiempo (Ti desde la compra hasta el consumo y prioridad (P para la compra (calidad o precio, entre otros ítems. Los encuestados manifestaron preferencias de consumo de espárragos verdes y en platos calientes, mostrando desinterés por el consumo de espárragos enlatados. En B, > 95 % demostró conocer las propiedades nutricionales y saludables de los espárragos. 71 % mostró interés por calibre mediano y un 75 % cortos (15-20 cm. Respecto de E, prefirieron las presentaciones en bandejas y atados con film, y con presencia de etiquetas, color de fondo llamativo (naranja y amarillo y letra negra. El 81,1 % adquieren espárragos mediante venta directa y 50 % manifestó que transcurre entre 2 días y 1 semana entre la adquisición y el consumo, prefiriéndolos frescos y no congelados. En P, el 90 % priorizó calidad en lugar del precio. Estos resultados indican que los consumidores no son indiferentes a los atributos de presentación del producto, por lo cual los empresarios deberían esforzarse por mejorar las dimensiones de la calidad del producto, por lo que se verían recompensados.

  20. Distribuição radicular de cultivares de aspargo em áreas irrigadas de Petrolina - PE Root distribution of asparagus cultivars in irrigated areas of Petrolina, Brazil

    Directory of Open Access Journals (Sweden)

    Luís Henrique Bassoi

    2001-03-01

    Full Text Available A distribuição radicular de duas cultivares de aspargo (New Jersey 220 e UC 157 F1, irrigadas por aspersão convencional, foi avaliada durante o ano de 1997 em solos de textura arenosa, em plantio experimental e comercial, respectivamente, nos Projetos de Irrigação de Bebedouro e Senador Nilo Coelho, em Petrolina (PE. O objetivo foi obter informações do sistema radicular do aspargo, empregando-se os métodos do monolito e do perfil de solo auxiliado pela análise de imagens digitais, para o manejo de solo e água nesse cultivo. Na área experimental, a maior parte da matéria seca, área e comprimento de raízes no perfil de solo e densidade de comprimento radicular foram encontradas até a profundidade de 0,4 m nas duas cultivares, enquanto que na comercial a maior parte da área e comprimento de raízes no perfil do solo estendeu-se até a profundidade de 0,6 m (cv. New Jersey 220. Nesses dois plantios, as raízes das cultivares atingiram a profundidade de 1 m. Na área experimental, a massa seca, a área e o comprimento no perfil de solo, e a densidade de comprimento radicular nas cultivares concentraram-se até a distância de 0,6 m à linha de plantas. No intervalo de diâmetro (d de raízes 2In 1997 the root distribution of two asparagus cultivars (New Jersey 220 and UC 157 F1 irrigated by sprinkler was evaluated in coarse textured soils in experimental and commercial areas at Petrolina county, in the semi-arid region of northeastern Brazil, to obtain useful information for soil and water management. Both the monolith and the soil profile aided by digital image analysis methods were used to evaluate it. In the experimental area, a greater concentration of root dry weight, root area and length in the soil profile and root length density were found up to 04 m depth for both cultivars, while in commercial area the root area and length of cv. New Jersey 220 were concentrated until 0,6 m depth. Roots reached 1 m depth in both areas. In

  1. 不同温度、光照及PEG-6000胁迫对南玉带种子萌发的影响%Effects of Temperature, Light Intensity and PEG Stress on Seed Germination of Asparagus oligoclonos

    Institute of Scientific and Technical Information of China (English)

    岳桦; 王海洋; 李玉珠

    2012-01-01

    以大庆草原干旱生境下采集的南玉带种子为试材,利用控制试验研究了不同光照、温度及聚乙二醇-6000(PEG-6000)胁迫对种子萌发的影响,为构建南玉带种子育苗高质量的技术流程提供依据.结果表明:15~30℃条件下南玉带种子都能够萌发,种子萌发温度范围具有宽适性,其中25℃为种子萌发最适温度;黑暗条件下种子萌发率达92.78%,较光照条件下萌发率高59.45%,其差异显著,说明黑暗条件较光照更适于种子萌发;南玉带种子萌发过程中随PEG-6000质量分数的升高,其萌发率、萌发指数、活力指数均呈下降趋势,但在PEG -6000质量分数为5%、10%处理下种子仍分别有22.78% 、51.11%的萌发率,说明南玉带种子萌发阶段具有一定的抗旱能力.%Asparagus oligoclonos seeds collected from arid grassland habitats in Daqing were used as test materials. The effects of temperature, light intensity, and water stress induced by polyethylene glycol (PEG-6000) on seed germination of A. oligoclonos were studied in order to provide a scientific basis for the technical process of high quality seedling cultivation from .4. oligoclonos seeds. Results show that the seeds of A, oligoclonos could germinate at 15-30 degrees C, indicating that the seed germination of A. oligoclonos has a wide range of adaptations to temperature. The most suitable temperature for seed germination is 25 degrees C. There is a significant difference in seed germination between dark conditions (92.78%) and light conditions (59.45%), which indicates that dark conditions are more suitable for seed germination. The germination rate, germination index, rigor index of .4. oligoclonos seeds exhibited downward trends with increasing PEG-6000 concentration during germination. However, the germination rates ranged from 22.78% to 51. 11% even under 5% -10% PEG-6000. The germination of A. oligoclonos seeds has a certain resistance to drought during

  2. Difference of fungicide resistance of asparagus stem blight fungus from different geographic origins%不同地理来源芦笋茎枯病菌对杀菌剂抗药性的差异

    Institute of Scientific and Technical Information of China (English)

    孟凡; 杨迎青; 兰波; 李湘民

    2013-01-01

    To define the difference of the resistance level of asparagus stem blight fungus Phomopsis asparagi (Sacc.) Bubak from different provinces on systemic and protective fungicides,the resistance levels of 24 isolates from 6 provinces on carbendazim and mancozeb were determined by using the mycelial growth rate method.The isolates from Shandong Province expressed the most resistance on carbendazim with an average EC50 value of 1.10 mg/L,followed by the isolates from Jiangxi Province and Fujian Province,with an average EC50 values of 0.58 mg/L and 0.33 mg/L respectively.The isolates from Fujian Province expressed the most resistance on mancozeb with an average EC50 value of 34.53 mg/L,followed by the isolates from Hebei Province and Shandong Province,with average EC50 values of 15.88mg/L and 14.19 mg/L respectively.Compared to mancozeb,it's easier for carbendazim to produce resistance,with 7 resistant isolates,3.66 as the resistant level and 29.17% as the resistant frequency,while 4 resistant isolates,with 1.90 as the resistant level and 16.67% as the resistant frequency,were included in mancozeb.Of 24 isolates,SD4 revealed resistance on both of carbendazim and mancozeb,with the resistance levels of 10.44 and 3.01 respectively,which expressed multiresistance.%为明确不同省份芦笋茎枯病菌对内吸性和保护性杀菌剂抗药性的差异,采用菌丝生长速率法测定了6个省24个菌株对多菌灵和代森锰锌2种杀菌剂的抗药水平,并比较了其差异.结果表明:各省份菌株对2种杀菌剂的抗药性均存在明显差异,其中山东省菌株对多菌灵的抗药性最强,平均EC50值为1.10 mg/L,江西省和福建省菌株次之,平均EC50值分别为0.58 mg/L和0.33 mg/L;福建省菌株对代森锰锌的抗药性最强,平均EC50值为34.53 mg/L,河北省和山东省菌株次之,平均EC50值分别为15.88 mg/L和14.19 mg/L.多菌灵相对代森锰锌更易产生抗药性,其抗药菌株为7个,平均抗性水平为3.66,

  3. Of Tangerines, Asparagus, and Nonprotein Biomolecules.

    Science.gov (United States)

    2016-05-19

    Every month the editors of Cell Chemical Biology bring you highlights of the most recent chemical biology literature. Our May 2016 selection includes a new method for labeling and visualizing nonprotein biomolecules using electron microscopy; a small molecule, originally found in tangerine peel, that regulates our circadian clock and helps with some of the metabolic pathologies associated with circadian rhythms; and a finding that asparagine is used as an amino acid exchanger in tumor cells. PMID:27203369

  4. 壳聚糖-La(Ⅲ)配合物的合成及对芦笋茎枯病菌抑菌活性研究%Synthesis of Chitosan-La (Ⅲ)complex and antifungal activity on hyphal growth of Phomopsis asparagus

    Institute of Scientific and Technical Information of China (English)

    才秀华; 张炜; 武侠

    2011-01-01

    Water-soluble complex of chitosan-La(Ⅲ)was synthesized and characterized by FT-IR and UV-Vis spectra. The antifugal activities of chitosan and chitosan-La(Ⅲ) complex against Phomopsis asparagus were investigated. Results showed that chitosan-La (Ⅲ)had the higher antifungal activity than water-soluble chitosan,and at the low level of concentation of 1. 00 mg · Ml-1 和 1. 25 mg · Ml-1,its activity was increased 87. 68% and 88. 23% respectively.%合成了水溶性壳聚糖和稀土离子La(Ⅲ)的配合物,利用FT-IR和UV-Vis对配合物进行了表征,研究了壳聚糖及其配合物对芦笋茎枯病菌Phomopsis asparagus的抑制活性.结果表明壳聚糖La(Ⅲ)配合物对Phomopsis asparagus的抑菌活性较单一的水溶性壳聚糖显著提高.在低浓度1.00 mg·mL-1和1.25 mg·mL-1条件下,其抑菌活性分别提高87.68%和88.23%.

  5. 固态发酵芦笋老茎生产高蛋白反刍动物饮料的研究%Study on solid-state fermentation of asparagus old stem for producing high quality protein feed of ruminant animals

    Institute of Scientific and Technical Information of China (English)

    王琳琳; 韩建荣; 李欣欣; 李艳婷

    2011-01-01

    通过单菌种固态发酵、复合菌种固态发酵以及不同配比的复合菌种固态发酵试验,对能够将芦笋老茎转化为反刍动物饲料的微生物菌种进行了筛选.结果表明,由青霉(Penicillium sp.)F-5、暗孢毛壳(Chaetomium atrosporum)F-21、曲霉(Aspergillus sp.)F-25菌株组成的配比为2∶2∶3的复合菌种是效果最好的菌种组合.芦笋老茎生料培养基经该复合菌种5d固态发酵后,发酵产物中可溶性蛋白含量达到4.80 mg/g,与对照组相比增加了39.94%;粗蛋白含量达到11.18%,与对照组相比增加了38.54%;纤维素由22.74%降低到22.32%,降低了1.85%;木质素由12.90%降低到10.53%,降低了18.37%.这些结果证明芦笋老茎经微生物发酵后能转化为良好的高蛋白反刍动物饲料.%By solid-state fermentation experiments with single strain and mixed strains, the microbial strains those could converted asparagus old stem into high -protein feed for ruminant animals. Were screened. The results showed that the strains combination of the ratio 2 : 2 : 3 of Penicillium sp. F-5, Chaetomium atrosporum F-21 and Aspergillus F-25 was proved to be the best combination. After solid-state fermentation for 5 days by this strains combination, in the fermented products, the soluble protein and crude protein contents reached respectively 4.80 mg/g and 11.18%, which was 39.94% and 38.54% higher than that of control; the cellulose content reduced from 22.74% to 22.32%, which was 1.85% lower than that of control; the lignin content reduced from 12.90% to 10.53%,which was 18.37% lower than that of control. Therefore, it could be concluded that the asparagus old stem could be converted into good high-protein feed for ruminant animals by solid-state fermentation.

  6. Effects of Pressurized CO2/Xe/O2 Mixture on the Storage Quality of Asparagus (Asparagus officinalis L. ) Spear%加压CO2/Xe/O2混合气体对芦笋贮藏特性的影响

    Institute of Scientific and Technical Information of China (English)

    单良; 单美; 田瑛; 张慜; 詹仲刚

    2008-01-01

    惰性气体产生笼形水合物的保鲜技术是一种极具开发前景的高新技术,而气调贮藏是当今最先进的果蔬保鲜贮藏方法之一.就低氧条件下加压CO2/Xe/O2混合气体对芦笋的保鲜效果进行研究,优化了保鲜条件,并进一步对其保鲜机制进行了初步探讨.

  7. Study on Changes of Cell Wall Polysaccharides and Phenols in Lignification of Green Asparagus (Asparagus Officinalis L.)%绿芦笋木质化过程中细胞壁多糖与酚类物质变化研究

    Institute of Scientific and Technical Information of China (English)

    刘尊英; 姜微波

    2005-01-01

    以绿芦笋栽培品种Mary Washington 500为试验材料,采用HPLC分析方法,研究了常温贮藏条件下,绿芦笋细胞壁多糖、酚类物质和木质素含量的变化.结果表明,随贮藏时间延长,绿芦笋细胞壁半纤维素的主要降解物木糖、岩藻糖残基增加了211.2%,纤维素的主要降解物葡萄糖残基增加了329.3%,木质素含量增加了280.3%.木质素合成前体物中,香豆酸含量最高,其次为咖啡酸和阿魏酸,三者的含量变化与木质素合成密切相关.

  8. Effect on Soil Nutrients of Planting Polygonatum sibiricum Red., Fallopia multilfora (Thunb.) and Asparagus cochinchinensis (Lour.) Merr. Under Forest%林下种植黄精、何首乌及天门冬对土壤养分的影响

    Institute of Scientific and Technical Information of China (English)

    何银忠; 王有兵; 严毅; 王亚婷; 周庆宏

    2014-01-01

    为了了解林下种植黄精、何首乌及天门冬对林地土壤养分的影响,为以后大规模发展林下中药材种植提供一定的理论指导,研究黄精等3种中药材根际土壤的养分变化。结果表明,黄精能够使根际土壤酸化,对照土壤中有机质含量较低,仅为34.0 g/kg,无法满足植物的生长需求。由于受有机质含量的影响,水解性氮总体上也偏低,有效磷和速效钾基本上能够满足这3种药材的生长。由于针叶难以腐化成有机质,今后应该选择阔叶林地或混交林地作为种植地,也可以通过人工增加土壤有机质含量来改良土壤性质。%Effects on soil nutrients of planting Polygonatum sibiricum Red., Fallopia multiflora (Thunb.) and Asparagus cochinchinensis (Lour.) Merr. under forest was studied. The aim of this study was to provide some theoretical guidance for after large-scale planting of Chinese herbal medicine under forest. Polygonatum sibiricum Red. can make the rhizosphere soil acidification. The low organic matter content in control soil of only 34.0g/kg is unable to meet the plant growth demand. Because of the influence of organic matter content, hydrolyzable nitrogen is generally low too, and available phosphorus and available potassium basically can satisfy the growth of these 3 kinds of herbs. Because the needles were difficult to decay into organic matter, so we should choose broad-leaved forest or mixed forest as planting fields. Also we can increase the content of soil organic matter artificially to improve the soil properties.

  9. Producción de primicia en invernadero de híbridos masculinos de espárrago (Asparagus officinalis y procesado IV Gama para optimización del posicionamiento en el mercado

    Directory of Open Access Journals (Sweden)

    Sofía Barreto

    2012-01-01

    Full Text Available El espárrago es una hortaliza muy valorada por sus propiedades, que ha experimentado un notable incremento durante los últimos años a nivel global, contando con una creciente demanda por parte de mercados muy exigentes respecto a su presentación y cada vez mas enfocados a adquirir productos listos para consumir. Con el objetivo de estudiar la productividad total (PBT y neta (PFN (kg/ha de espárrago verde bajo cubierta, el periodo de poscosecha (PPC y la pérdida de peso fresco diaria (PPFD promedio de turiones acondicionados en bandejas con film (B y atados sin film (A, se realizó un ensayo para comparar la producción de primicia en invernadero, de cuatro híbridos masculinos de espárrago verde, en la Facultad de Ciencias Agrarias de la UCA, en Buenos Aires, Argentina. La mayor producción neta (PFN se obtuvo con el híbrido Italo: 8.925,00 kg/ha, seguido por Zeno: 5.558,68; UC-157: 5.267,02; Eros: 4.937,75 y Ercole: 3.099,02 kg/ha (p < 0,05. En general se observó una mayor precocidad en los híbridos de origen italiano respecto del UC-157, único de origen americano. El peso fresco de los turiones de espárrago verde, acondicionados en bandejas, se conservarían por más tiempo sin alterar su peso inicial significativamente respecto del uso de atados. Con el empleo de bandejas, el PPC resultó 22 días mayor (A: 13 y B: 35. La PPFD promedio para ambas presentaciones, fue inferior en Italo y Zeno (1,35 y 1,50 % diaria, respecto de Ercole y Eros (1,99 y 2,04 %, respectivamente. La conservación del peso fresco (CPP para los distintos híbridos, mediante A, a lo largo de los 13 días de poscosecha fue: Italo: 76,91 %; Zeno: 69,34 %; Ercole: 64,71 %; Eros: 61,51 %. En el caso de B, a lo largo de los 35 días fue de: Zeno: 77,63 %; Italo: 67,95 %; Eros: 61,15 %; Ercole: 55,38 %. Estos resultados demuestran la mayor productividad de primicia mediante el empleo de Italo, Zeno, UC-157, Eros y Ercole, y; en poscosecha, la conveniencia del empleo de la técnica IV Gama para la optimización de la calidad del producto final y la extensión del periodo de oferta, destacándose los híbridos Zeno e Italo.

  10. 正交试验优选超声提取芦笋总皂苷的工艺%Optimization of Ultrasound Extraction Process of the Total Saponins from Asparagus officinalis L.by Orthogonal Test

    Institute of Scientific and Technical Information of China (English)

    揭晶; 王皓; 蔡由生

    2014-01-01

    目的:优选超声提取芦笋总皂苷的最佳工艺.方法:以高氯酸作为显色剂,用紫外分光光度法测定芦笋中总皂苷的含量,并以提取率为评价指标,采用单因素实验和正交试验优选最佳提取工艺.结果:芦笋总皂苷超声提取的最佳工艺为乙醇浓度70%,料液比1:15 (W/V),超声时间50 min,超声温度40℃.结论:该提取工艺可行,为芦笋总皂苷的进一步研究提供了依据.

  11. Optimization of Ultrasound Extraction Technology for Total Saponins from Asparagus officinalis by Box-Behnken Response Surface Methodology%Box-Behnken响应面法优化芦笋总皂苷超声提取工艺

    Institute of Scientific and Technical Information of China (English)

    揭晶; 赵越; 王皓

    2014-01-01

    目的:优选芦笋总皂苷的超声提取工艺.方法:以总皂苷含量为指标,在单因素试验基础上,通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对提取工艺的影响.结果:最佳超声提取工艺为加15倍量74%乙醇于50℃超声提取54 min;总皂苷平均质量分数13.059%(RSD 1.63%),与预测值(13.185%)的偏差较小.结论:优选的提取工艺稳定可行,为芦笋总皂苷的开发应用提供实验依据.

  12. 响应面优化超声辅助提取芦笋中总皂苷的工艺研究%Response Surface Optimization of Ultrasound Extraction Technology for the Total Saponins from Asparagus officinalis L.

    Institute of Scientific and Technical Information of China (English)

    王皓; 揭晶

    2014-01-01

    利用响应面法优化芦笋总皂苷的超声提取工艺研究.通过单因素试验分析了提取过程中乙醇体积分数、料液比、超声时间、超声温度4个因素对芦笋总皂苷提取率的影响.在单因素试验的基础上,采用响应面分析获得了最佳超声提取条件,即乙醇体积分数74%,超声时间54 min,超声温度50℃.在此条件下,芦笋总皂苷提取率为13.059%,接近于模型预测值13.185%.

  13. Producción de primicia en invernadero de híbridos masculinos de espárrago (Asparagus officinalis y procesado IV Gama para optimización del posicionamiento en el mercado

    Directory of Open Access Journals (Sweden)

    Sofía Barreto

    2012-12-01

    Full Text Available El espárrago es una hortaliza muy valorada por sus propiedades, que ha experimentado un notable incremento durante los últimos años a nivel global, contando con una creciente demanda por parte de mercados muy exigentes respecto a su presentación y cada vez mas enfocados a adquirir productos listos para consumir. Con el objetivo de estudiar la productividad total (PBT y neta (PFN (kg/ha de espárrago verde bajo cubierta, el periodo de poscosecha (PPC y la pérdida de peso fresco diaria (PPFD promedio de turiones acondicionados en bandejas con film (B y atados sin film (A, se realizó un ensayo para comparar la producción de primicia en invernadero, de cuatro híbridos masculinos de espárrago verde, en la Facultad de Ciencias Agrarias de la UCA, en Buenos Aires, Argentina. La mayor producción neta (PFN se obtuvo con el híbrido Italo: 8.925,00 kg/ha, seguido por Zeno: 5.558,68; UC-157: 5.267,02; Eros: 4.937,75 y Ercole: 3.099,02 kg/ha (p < 0,05. En general se observó una mayor precocidad en los híbridos de origen italiano respecto del UC-157, único de origen americano. El peso fresco de los turiones de espárrago verde, acondicionados en bandejas, se conservarían por más tiempo sin alterar su peso inicial significativamente respecto del uso de atados. Con el empleo de bandejas, el PPC resultó 22 días mayor (A: 13 y B: 35. La PPFD promedio para ambas presentaciones, fue inferior en Italo y Zeno (1,35 y 1,50 % diaria, respecto de Ercole y Eros (1,99 y 2,04 %, respectivamente. La conservación del peso fresco (CPP para los distintos híbridos, mediante A, a lo largo de los 13 días de poscosecha fue: Italo: 76,91 %; Zeno: 69,34 %; Ercole: 64,71 %; Eros: 61,51 %. En el caso de B, a lo largo de los 35 días fue de: Zeno: 77,63 %; Italo: 67,95 %; Eros: 61,15 %; Ercole: 55,38 %. Estos resultados demuestran la mayor productividad de primicia mediante el empleo de Italo, Zeno, UC-157, Eros y Ercole, y; en poscosecha, la conveniencia del empleo de la técnica IV Gama para la optimización de la calidad del producto final y la extensión del periodo de oferta, destacándose los híbridos Zeno e Italo.

  14. Optimization of the extraction of polyphenolics from young stems of Asparagus officinalis using orthogonal array design%正交实验设计优化芦笋嫩茎多酚的提取工艺

    Institute of Scientific and Technical Information of China (English)

    朱素英

    2013-01-01

    以芦笋嫩茎为研究材料,采用单因素和正交实验设计对芦笋多酚提取工艺进行优化,考察乙醇浓度、提取时间、提取温度以及料液比对芦笋多酚提取率的影响.结果显示,芦笋多酚提取的最佳工艺条件为,料液比1∶15、50%的乙醇、80℃水浴条件下提取60min,在该条件下,芦笋多酚的得率为6.90%.该最佳工艺费用低,操作简便,可为芦笋的加工利用提供技术上的支持.

  15. Changes of Dried and Fresh Leaf Weight Ratio of Asparagus officinalis During Its Growth Period%芦笋生长过程中叶片干鲜比值变化

    Institute of Scientific and Technical Information of China (English)

    张俐英

    2010-01-01

    对芦笋不同部位叶片生长发育过程中叶片干鲜比值的变化进行了研究.结果表明:在芦笋叶片生长发育过程中,随着叶片的生长,叶片干鲜比值表现为随之增加的变化规律,不同部位叶片干鲜比值的差异显著,而不同叶龄之间叶片干鲜比值的差异不显著.随着叶龄的增大,各部位叶片干鲜比值的变化不尽一致,分析表明,叶龄与叶片干鲜比值之间呈不显著正相关.

  16. 芦笋茎叶游离氨基酸的提取及含量测定%Free Amino Acid Extraction and Content Determination of Asparagus officinalis L. Stem and Leaves

    Institute of Scientific and Technical Information of China (English)

    黄晓德; 赵伯涛; 钱骅

    2006-01-01

    通过对芦笋茎叶游离氨基酸提取工艺中温度、提取时间、料液比、乙酸浓度等影响因素的试验分析,确定芦笋茎叶游离氨基酸测定过程中的最佳提取条件为:提取温度60 ℃;料液比为1∶35;提取时间为2.5 h;并对芦笋茎叶游离氨基酸含量进行了测定,确定芦笋茎叶游离氨基酸含量为76.54 mg/100 g.

  17. The Development of Chemical Components in Asparagus officinalis and Their Medical Effects%芦笋的化学成分及药理作用研究进展

    Institute of Scientific and Technical Information of China (English)

    孙春艳; 赵伯涛; 郁志芳; 钱骅; 黄晓德

    2004-01-01

    本文综述了芦笋中含有的多种化学成分,及近年来国内外对芦笋的抗肿瘤、降血脂、免疫作用、抗衰老、抗疲劳等药理作用的研究进展,并简要阐述了芦笋的综合利用情况.

  18. Selenium enrichment pattern in flowering Chinese cabbage, cabbage and asparagus

    NARCIS (Netherlands)

    Mo, H.Z.; Yang Zhu, Yang; Zhang, M.

    2006-01-01

    CONCLUSIONS - Within a certain range, selenium accumulation in three studied vegetables was lineally correlated with spraying concentration. However, a too high concentration caused the reduction of vegetable output and damage in quality. - Twice spraying with lower concentration of selenium was a b

  19. 名贵蔬菜-芦笋

    Institute of Scientific and Technical Information of China (English)

    胡平; 鲁旭东

    2006-01-01

    @@ 芦笋学名石刁柏(Asparagus Officinalis L.),英文名为Asparagus,俗称芦笋、龙须菜,为百合科天门冬属多年生宿根草本植物,以抽生的嫩茎为蔬菜食用.因其风味独特,营养价值高而深受消费者的欢迎.

  20. 文竹(Asparagus plumosus Baker.)快速繁殖的初步研究%Primary study on fast propagation of Asparagus plumosus Baker.

    Institute of Scientific and Technical Information of China (English)

    吴均章; 万志刚

    2000-01-01

    文竹传统的繁殖方法主要为播种,结合分株.本文采用组织培养的方法,对文竹的快速繁殖进行初步的研究.结果发现不同的外植体材料,不同的培养基配方对文竹的生长都产生很大的影响.并初步建立了文竹的快繁体系.

  1. Productividad de una plantación de espárrago verde (Asparagus officinalis var. altilis L. con diferentes tamaños de “arañas” y densidades, en su séptimo año

    Directory of Open Access Journals (Sweden)

    J. Marina

    2012-01-01

    Full Text Available El espárrago es un cultivo rústico que tradicionalmente se ha iniciado mediante el sistema tradicional de “arañas”. El presente ensayo tuvo como objetivo evaluar la productividad total y comercial de una plantación de espárrago verde al séptimo año desde la plantación. El ensayo de 1.728 m2 está ubicado en la Chacra Experimental de la Facultad de Agronomía de la UNCPBA (36º 48’ latitud Sur y 59º 51’ longitud Occidental, en Azul, Argentina. La plantación, iniciada el 12/09/2003, se evaluó en el período 17/09/2010-01/11/2010. Las variables estudiadas fueron: tamaños de “arañas” grandes (AG > 200 g, medianas (AM 100-200 g y pequeñas (AP 0,05. El rendimiento general del ensayo fue PFT: 19.758 y PFN: 10.018 kg/ha; mientras que NTT: 483.425 y NTC: 477.200. En PFT se observaron diferencias significativas (p 0,05, siendo la media en PFN: 9.910 kg/ha. En densidad y en tamaño de “arañas” los resultados difirieron de los encontrados 2 años anteriores en los que se destacaron mayor densidad y tamaño grande; efectos atribuibles a la competencia intraespecífica.

  2. Productividad de una plantación de espárrago verde (Asparagus officinalis var. altilis L. con diferentes tamaños de “arañas” y densidades, en su séptimo año

    Directory of Open Access Journals (Sweden)

    A. M. Castagnino

    2012-12-01

    Full Text Available El espárrago es un cultivo rústico que tradicionalmente se ha iniciado mediante el sistema tradicional de “arañas”. El presente ensayo tuvo como objetivo evaluar la productividad total y comercial de una plantación de espárrago verde al séptimo año desde la plantación. El ensayo de 1.728 m2 está ubicado en la Chacra Experimental de la Facultad de Agronomía de la UNCPBA (36º 48’ latitud Sur y 59º 51’ longitud Occidental, en Azul, Argentina. La plantación, iniciada el 12/09/2003, se evaluó en el período 17/09/2010-01/11/2010. Las variables estudiadas fueron: tamaños de “arañas” grandes (AG > 200 g, medianas (AM 100-200 g y pequeñas (AP 0,05. El rendimiento general del ensayo fue PFT: 19.758 y PFN: 10.018 kg/ha; mientras que NTT: 483.425 y NTC: 477.200. En PFT se observaron diferencias significativas (p 0,05, siendo la media en PFN: 9.910 kg/ha. En densidad y en tamaño de “arañas” los resultados difirieron de los encontrados 2 años anteriores en los que se destacaron mayor densidad y tamaño grande; efectos atribuibles a la competencia intraespecífica.

  3. Salvia officinalis in dentistry

    Directory of Open Access Journals (Sweden)

    Nikhita Narayanan

    2015-01-01

    Full Text Available Salvia officinalis is a medicinal herb used as an cosmetic, flavoring agent, It has antibacterial, antifungal, anticaries, antiplaque, antiviral, astringent, and other useful properties, it is also used in dental practice for the management of periodontal disease and to prevent halitosis. The objective of this article is to highlight various uses of S. officinalis in the dental field along with its use in medical problems.

  4. Drug: D06766 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 36 Therapeutic category: 5100 Asparagaceae (asparagus family) Asparagus Tuber Maj...hing Ying Drugs for replenishing Ying D06766 Asparagus tuber Crude drugs [BR:br08305] Monocot plants Asparagaceae (asparagus family) D06766 Asparagus tuber PubChem: 47208417 ...

  5. Effect of dietary supplementation of Shatavari (Asparagus racemosus wild On heamatobiochemical parameters of broilers

    Directory of Open Access Journals (Sweden)

    Rekhate

    2010-12-01

    Full Text Available Eighty, day old straight run commercial broilers chicks were randomly distributed into four groups (T1, T2, T3 and T4 of two replications having ten chicks in each, reared on deep litter system upto 8 weeks of age. The chicks in all the treatment groups were fed basal diet T1 control and T2, T3 and T4 groups were fed basal diet + 0.5, 1.00 and 1.5 per cent Shatavari root powder (SRP respectively. Significant (P<0.01 improvement in live body weight of broilers was observed between the treatments. Better FCE was observed with the increase in SRP. The digestibility of protein found significantly (P< 0.01 better with higher inclusion level of SRP. Hb, total serum protein, albumin and globulin revealed significant (P< 0.01 variation between the treatments. It was concluded that supplementation of Shatavari root powder at 0.5, 1.00 and 1.5 per cent level improves growth performance and general health status of broilers however, 1.0 per cent level could be more economical. [Vet. World 2010; 3(6.000: 280-281

  6. Effect of Asparagus racemosus, Sida cordifolia and Levamisole on immunological parameters in experimentally induced mmunosuppressed broilers.

    Directory of Open Access Journals (Sweden)

    Tekade S. H.

    2008-04-01

    Full Text Available In birds treated with Cyclophosphamide @ 150 mg/kg body weight I/V on 21st day of age produced marked immunosuppression. Administration of A. racemosus, Sida cordifolia in combination with Levamisole was the more effective in producing immunomodulatory effect in immunosuppressed birds. [Veterinary World 2008; 1(2.000: 49-50

  7. Selección de progenitores en espárrago Parent selection in asparagus

    OpenAIRE

    I. Gatti; F. López Anido; L. Picardi; Cointry, E.

    2003-01-01

    O objetivo principal deste trabalho foi determinar as variáveis que melhor explicam a variação entre indivíduos dentro da população de aspargos originária da cultivar Argenteüil e selecionar plantas superiores para compor novas populações. O ensaio, composto por 1280 plantas, foi instalado no campo experimental da Facultad de Ciencias Agrarias de la Universidad Nacional de Rosario, Santa Fe, Argentina, em 1996. Durante os anos de 1997 e 1998, foram avaliadas as variáveis: número de turiões po...

  8. 芦笋天然功能饮料%Asparagus Juice--Natural and Functional Beverage

    Institute of Scientific and Technical Information of China (English)

    郭海燕; 周芳

    2002-01-01

    以芦笋为主要原料,应用正交试验设计开发出色泽亮丽、柔和爽口、低糖、低热的保健型饮料,同时对产品的生产工艺进行了研究,确定了最佳生产配方,成功解决了产品的稳定性问题.

  9. Effect of water extract from traditional Chinese medicines Rehmannia glutinosa, Scrophularia ningpoensis, Asparagus cochinchinensis and Ophiopogon japonicas on contents of CYP450 and activities of CYP3A,CYP2E1 and CYP1A2 in rat%天王补心丸中生地黄、玄参、天冬和麦冬水提液对大鼠肝CYP450酶含量及其亚型CYP3A,CYP2E1和CYP1A2活性的影响

    Institute of Scientific and Technical Information of China (English)

    武佰玲; 刘萍; 高月; 王宇光

    2011-01-01

    Objective; To study the effects of the water extract of Rehmannia glulionsa, Scrophularia ningpoensis, Asparagus cochinchinensis and Ophiopogon japoiucas, which are the drug from Tianwang Buxin Wan from nourishing vin, on the content of cyto-hrome P450(CYP450) in rat and the activities of CYP3A, CYP2E1 and CYP1A2 to investigate the role of CYP450 in the biotransfor-mation of Tianwang Buxin Wan. Method: The rats were killed after administrated with extracts once daily for consecutive 7 days, the livers were removed rapidly and weighed, liver microsomes were prepared with ultra-centrifuge method, the contents of liver microsomal CYP450, cytochrome b5 ( Cytb5) and the activities of CYP3A were examined by ultraviolet spectrophotometry, the activities of CYP2E1 and CYP1A2 were determined by high performance liquid chromatography (HPLC). Result; AD groups had no difference in the levels of liver indexe compared with normal sodium group, the water extract of R. glulionsa obviously decreased the contents of P4S0 (P < 0.01) and increased the activity of CYP3A( P < 0.01) and CYP1A2 (P < 0.05). The water extract of S. ningpoensis decreased the contents of P450(P<0.05) and significantly increased CYP3A and CYP1A2 activities (P <0.01). A. cochinchinensis increased content of Cytb5 ( P < 0.05) in rat and increased the activity of CYP2E1 ( P < 0.05 ) and CYP1A2 (P < 0.01). O. japonkas had no significant difference on the contents of CYP450 and Cytb5 while increased the activities of CYP3A ( P < 0.05 ) , CYP2E1 ( P < 0.05 ) and CYP1A2 (P <0.01). Conclusion; R. glutionsa and 5. ningpoensis could decrease the content of CYP450 enzyme in rat liver and induct the activities of CYP3A and CYP1A2. A. cochinchinensis could induct the activities of CYP2E1 and CYP1A2. O. japonicus could induction the activities of CYP3A, CYP2E1 and CYP1A2 in Tianwang Buxin Wan. By inhibiting CYP450 activity to decrease the metabolism of other drugs, the effect of other functional groups in the compatibility

  10. Efficacy of Asparagus recemosus (Satavar in stimulating follicular growth and ovulation in anovulatory infertility: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Sana Fatima Majeedi

    2016-02-01

    Conclusions: The effect of test drug was comparable with that of control drug in stimulating follicular growth and ovulation, though the drug was not as effective as control drug to achieve conception. [Int J Reprod Contracept Obstet Gynecol 2016; 5(2.000: 310-316

  11. 芦笋营养成分分析研究%Research analysis of the nutritional labeling of the asparagus

    Institute of Scientific and Technical Information of China (English)

    叶春勇; 林媚

    2005-01-01

    前言:芦笋,学名石刁柏(Aspargns,ofIicimlis L.)别名龙须菜,是天门冬属百合科宿根性多年生草本植物。取食内茎,是一种具有较高药用价值的营养蔬菜和保健食品。我国的《神农本草经》将芦笋列为“上品之上”。原野生于地中海东部沿岸和小亚细亚一带。因其脆嫩多汁,清香爽口,欧洲人喜食,由野生变为人工栽培。随欧洲移民传入亚洲、美洲,澳洲等地,

  12. Research on salinization of asparagus%葡甘露聚糖在食品中的应用

    Institute of Scientific and Technical Information of China (English)

    王蕾娜; 康明丽

    2012-01-01

    介绍了葡甘露聚糖的结构及性质,阐述了葡甘露聚糖的发展现状,综述了葡甘露聚糖的优良性质及在食品领域中的应用,并对葡甘露聚糖的应用进行了展望。%Introduce the structure and nature of glucomannan and state the current development status of glucomannan. Review these excellent properties of the glucomannan and its application in food, predict the application of glucomannan.

  13. DNA endoreplication level in endosperm during seed development in three monocotyledonous species

    Directory of Open Access Journals (Sweden)

    Kazimierz Marciniak

    2014-02-01

    Full Text Available The DNA content after the Feulgen reaction in the endosperm of three monocotyledonous plant species (Asparagus officinalis, Muscari comosom, Haemanthus kurharinae differing in their 2C DNA content, was cytophotometrically measured. During endosperm development 1-6 endoreplication cycles take place, depending on the species. Differences in nuclear DNA endoreplication dynamics in the tested species are similar to those occurring in root parenchyma, but the endoreplication level in the endosperm is higher.

  14. Environ: E00739 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available [TAX:100532], Dracaena [TAX:39502] Asparagaceae (asparagus family) Dracaena ombet, Dracaena cinnabari, Drac...aena draco resin Crude drugs [BR:br08305] Monocot plants Asparagaceae (asparagus family) E00739 Dracaena resin ...

  15. Diabetes-Friendly Recipes

    Science.gov (United States)

    ... well as your taste buds. Lemony Tilapia and Asparagus Grill A combination of chili powder and lemon ... vinegar and oil mixture adds flair to the asparagus. Sides Tex Mex Corn and Zucchini Colorful and ...

  16. Vitamin Chart

    Science.gov (United States)

    ... lentils), nuts, dairy products, green leafy vegetables, broccoli, asparagus, and fortified cereals. Teen guys need 1.3 ... dried beans and other legumes, green leafy vegetables, asparagus, and orange juice are good sources of this ...

  17. Shaking the Salt Habit

    Science.gov (United States)

    ... seeds: Lean meats, stews, soups, salads, breads, cabbage, asparagus, noodles Chives: Salads, sauces, soups, lean meat dishes, ... Lean ground meats, lean meats, chicken, fish, salads, asparagus, broccoli, Brussels sprouts, cabbage, mayonnaise, sauces Nutmeg: Fruits, ...

  18. PHYTOCHEMICAL SCREENING OF CALENDULA OFFICINALIS LINN

    Directory of Open Access Journals (Sweden)

    Chakraborthy Guno Sindhu

    2010-09-01

    Full Text Available Identification of primary and secondary constituents has become the utmost important tool for the presence of active moiety. The phytochemical screening of petroleum ether, chloroform, methanol and water extracts of Calendula officinalis leaf done by TLC means. Petroleum ether extract showed the presence of fatty acids, chloroform extracts showed the presence of triterpens and sterols. Flavonoids, carbohydrates, amino acids and saponins were present in methanol extract and saponins, phenolic substances and tannins were present in the water extract of Calendula officinalis.

  19. Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.)

    OpenAIRE

    JASNA IVANOVIĆ; SONJA ĐILAS; MILKA JADRANIN; VLATKA VAJS; NADA BABOVIĆ; SLOBODAN PETROVIĆ; IRENA ŽIŽOVIĆ

    2009-01-01

    The aim of the present study was to isolate and characterize antioxidant extracts obtained from dried leaves of rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.), originating from the southern Balkan Region. The antioxidant fraction was isolated from the plant material by supercritical carbon dioxide (SC-CO2) fractional extraction under a pressure of 30 MPa and at temperatures of 40 and 100 °C. In the present study, kinetic data and yields of antioxidant extracts obtained ...

  20. 75 FR 41433 - Trade Adjustment Assistance for Farmers

    Science.gov (United States)

    2010-07-16

    ... petition (No. 2010003) for trade adjustment assistance (TAA) for asparagus that was filed by the National Asparagus Council and accepted for review by USDA on May 3, 2010. Individual producers, nationwide, will be... review, the Administrator determined that increased imports of asparagus during January-December...

  1. 75 FR 23226 - Trade Adjustment Assistance for Farmers

    Science.gov (United States)

    2010-05-03

    ..., and began a review of a petition, for trade adjustment assistance by the National Asparagus Council on behalf of asparagus producers in California, Michigan, and Washington. The Administrator will determine within 40 days whether or not increasing imports of asparagus contributed importantly to a greater...

  2. Borago officinalis L. (fotografía)

    OpenAIRE

    Mallor Giménez, Cristina

    2013-01-01

    Front cover: Borago officinalis L., one of the priority crop wild relative species of Spain. Cultivated forms are grown along the Ebro Valley in the northeast. Its basal leaf petioles are used as a vegetable. Borage is also a valuable medicinal plant with a high content of gamma linolenic acid

  3. [Chemical constituents of Morinda officinalis How].

    Science.gov (United States)

    Li, S; Ouyang, Q; Tan, X; Shi, S; Yao, Z

    1991-11-01

    This paper reports the identification of four compounds isolated from the cortex of Morinda officinalis growing in Guangdong Province. These compounds are beta-sitosterol (I), 2-methyl-anthraquinone(II), rubiadin-1-methyl ether(III) and 24-ethylcholesterol(VI). Compounds II and VI are isolated from Morinda Genus for the first time. PMID:1804172

  4. Neuroprotective and neurological properties of Melissa officinalis

    DEFF Research Database (Denmark)

    López, Víctor; Martín, Sara; Gómez-Serranillos, Maria Pilar;

    2009-01-01

    Melissa officinalis has traditionally been used due to its effects on nervous system. Both methanolic and aqueous extracts were tested for protective effects on the PC12 cell line, free radical scavenging properties and neurological activities (inhibition of MAO-A and acetylcholinesterase enzymes...

  5. Development of mouthwash with Rosmarinus officinalis extract

    Directory of Open Access Journals (Sweden)

    Isabela Moreira Baumgratz de Paula

    2014-12-01

    Full Text Available Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quality of the drugs were carried out, quantifying phenols and flavonoids as chemical markers. Mouthwash solutions were formulated containing 0.1, 5 and 10% ethanol extract of R. officinalis; and 0.05, 5 and 10% of the hexane fraction of R. officinalis. In order to evaluate synergism, ethanol extract and hexane fraction were also added to formulations containing 0.05% sodium fluoride and 0.12% chlorhexidine digluconate. These formulations were assessed for inhibitory effect against the specific microorganisms involved in the process of bacterial plaque formation, S. mutans(ATCC25175 and C. albicans(ATCC 10231, frequently found in cases of oral infections. The agar diffusion method was used to evaluate the inhibitory activity of extracts and formulations. All mouthwash solutions displayed inhibitory activity having higher sensitivity to S. mutansfor the 5% ethanol extract+0.05% sodium fluoride, and greater sensitivity to C. albicansfor the 10% hexane fraction. Results were characterized by the appearance of a growth inhibition halo, justifying the utilization and association of extracts of R. officinalis.

  6. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    OpenAIRE

    Vantu Smaranda

    2015-01-01

    The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae) is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and tr...

  7. Obtenção de um compósito a partir da biomassa residual e industrial utilizando um reator de contracorrente

    Directory of Open Access Journals (Sweden)

    Felix Martin Carbajal Gamarra

    2012-01-01

    Full Text Available The major preoccupation and challenge for researchers is finding sustainable alternatives for the reuse of biomass. In view of this, the goal of this research was to produce a new compact material with minimum defects in its anatomic structure and excellent particle-resin interaction. The material obtained is a type of MDF panel composite made from industrial solid vegetable biomass. The MDF panel was produced using a vegetable biomass blend of Eucalyptus wood (Eucalyptus sp., asparagus (Asparagus officinalis, sugar-cane (Saccharum officinarum, and synthetic resin from urea-formaldehyde (UF. Physical-mechanical properties of the MDF, including modulus of rupture, strength, modulus of elasticity, density, thermal conductivity and perpendicular traction, were evaluated following EN and ASTM norms. The results obtained were consistent with and, in some cases, superior to those found in the literature. Thus, this research demonstrated the potential sustainable reuse of vegetal biomass to produce useful panels.

  8. Arabidopsis defense response against Fusarium oxysporum.

    Science.gov (United States)

    Berrocal-Lobo, Marta; Molina, Antonio

    2008-03-01

    The plant fungal pathogen Fusarium oxysporum (Fox) is the causal agent of root rot or wilt diseases in several plant species, including crops such as tomato (Solanum lycopersicum), banana (Musa sapientum) and asparagus (Asparagus officinalis). Colonization of plants by Fox leads to the necrosis of the infected tissues, a subsequent collapse of vascular vessels and decay of the plant. Plant resistance to Fox appears to be monogenic or oligogenic depending on the host. Perception of Fox by plants follows the concept of elicitor-induced immune response, which in turn activates several plant defense signaling pathways. Here, we review the Fox-derived elicitors identified so far and the interaction among the different signaling pathways mediating plant resistance to Fox. PMID:18289920

  9. MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS

    Directory of Open Access Journals (Sweden)

    Ahmad F

    2012-04-01

    Full Text Available Paeonia officinalis (European peony, Common peony has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root is also antispasmodic, diuretic, sedative and tonic and has been successfully employed in the treatment of convulsions and spasmodic nervous affections such as epilepsy. It has also been used in the treatment of whooping cough whilst suppositories are sometimes made of the root to relieve anal and intestinal spasms, hemorrhoids and varicose veins. Experimentally it has been proved to have antihypertensive, abortifacient action and anti-ulcer activity. The roots of this plant are of great medicinal significance in unani system and homeopathy. The roots contain asparagin, benzoic acid, flavonoids, paeoniflorin, paeonin, paeonol, protoanemonin, tannic acid, triterpenoids, and volatile oil. This review covers botany, traditional uses and the phytoconstituents of the roots of Paeonia officinalis.

  10. MEDICINAL USES AND PHYTOCONSTITUENTS OF PAEONIA OFFICINALIS

    OpenAIRE

    Ahmad F; Tabassum N; Rasool S

    2012-01-01

    Paeonia officinalis (European peony, Common peony) has been cultivated in Europe for years. The root has been used medicinally for over 2,000 years mainly in the treatment for epilepsy and to promote menstruation. Root is also antispasmodic, diuretic, sedative and tonic and has been successfully employed in the treatment of convulsions and spasmodic nervous affections such as epilepsy. It has also been used in the treatment of whooping cough whilst suppositories are sometimes made of the root...

  11. Diverse Belongings of Calendula officinalis: An Overview

    OpenAIRE

    M U Khan; Ankur Rohilla; Deepika Bhatt; Shadan Afrin; Seema Rohilla; Ansari, S. H.

    2011-01-01

    Herbal drugs therapy is regarded as an important therapy for the treatment of a wide range of diseases. However, enormous improvements have been observed in modern systems of medicine, but Indian herbal plants provide a rich source for health care to prevent different pathological states. Calendula officinalis is an aromatic, erect, annual herb that is indigenous to Europe, cultivated commonly in North America, Balkans, Eastern Europe, Germany and India. Ample studies have reported about the ...

  12. PHYTOCHEMICAL SCREENING OF CALENDULA OFFICINALIS LINN

    OpenAIRE

    Chakraborthy Guno Sindhu

    2010-01-01

    Identification of primary and secondary constituents has become the utmost important tool for the presence of active moiety. The phytochemical screening of petroleum ether, chloroform, methanol and water extracts of Calendula officinalis leaf done by TLC means. Petroleum ether extract showed the presence of fatty acids, chloroform extracts showed the presence of triterpens and sterols. Flavonoids, carbohydrates, amino acids and saponins were present in methanol extract and saponins, phenolic ...

  13. Development of mouthwash with Rosmarinus officinalis extract

    OpenAIRE

    Isabela Moreira Baumgratz de Paula; Flávia Costa Moraes; Orlando Vieira de Souza; Célia Hitomi Yamamoto

    2014-01-01

    Rosmarinus officinalis, which belongs to the Lamiaceaefamily, is a species of medicinal flora with therapeutic properties. In order to exploit the benefits of these properties, a mouthwash formulation was developed, with careful selection of raw materials to meet pharmacotechnical requirements. Extracts of the plant were incorporated into a mouthwash, which was shown to have inhibitory action in vitro against the micro-organisms commonly found in periodontics. Controls for assessing the quali...

  14. Evaluation of analgesic activity of Emblica officinalis in albino rats

    Directory of Open Access Journals (Sweden)

    Bhomik Goel

    2014-04-01

    Results: Emblica officinalis extract did not produced statistically significant (p>0.05 analgesia when compared with the control group in hot plate latency, but produced a statistically significant reduction in 6% NaCl induced abdominal writhing (pEmblica officinalis exhibit analgesic activity involving peripheral mechanisms. [Int J Basic Clin Pharmacol 2014; 3(2.000: 365-368

  15. [Experimental studies on growing seedlings of Morinda officinalis How].

    Science.gov (United States)

    Wei, X; Pang, F; He, M; Hu, T

    1992-10-01

    By using the techniques of growing seedlings in bowshaped shed covered with plastic sheeting plus treatment with plant growth regulator, vegetative and generative propagation tests have been made of Morinda officinalis. Scientific evidences have thus been provided for growing seedlings of Morinda officinalis. PMID:1294174

  16. Baeuveria bassiana (BalsVuill and Metarhizium anisioplae (Metsch. Sorokin in the pupas control of Prodiplosis longifila Gagné on asparagus crop

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    Carolina Cedano

    2012-03-01

    Full Text Available The objective of the present work was to evaluate the effect of the entomopathogen fungi Baeuveria bassiana and Metarhizium anisioplae of the Prodiplosis longifila pupas, in order to reduce the adult population of this insect. The treatments consisted on the application of two different propagel concentrations (mycel and conidia of each entomopathogen. One of these concentration was 1x106 propagels/ml (obtained from the total amount of conidia collected of 25 kg of rice colonized by the entomopathogen; and the other one, was 1x107 propagels/ml (obtained from the total amount of conidia collected of 40 kg of rice colonized by the entomopathogen. As a check a non application treatment was considered. The applications were trough the irrigation system and started 15 days after the end of harvest and were repeated each five days during a month, coincident with the period of most falls of pupas of the soil. As a result Baeuveria bassiana at 1x107 propagels/ml shown 53.4 % of the total pupas colonized by the entomopathogen, which allows indicating B. bassiana as a promissory biocontrol of this specie

  17. Asparagus Sorter (agric.; can. & preserv.; whole tr.) 529.687 (8-04.10)--Technical Report on Development of USES Aptitude Test Battery.

    Science.gov (United States)

    Manpower Administration (DOL), Washington, DC. U.S. Training and Employment Service.

    The United States Training and Employment Service General Aptitude Test Battery (GATB), first published in 1947, has been included in a continuing program of research to validate the tests against success in many different occupations. The GATB consists of 12 tests which measure nine aptitudes: General Learning Ability; Verbal Aptitude; Numerical…

  18. Practical Processing Technique of Asparagus Purified Liquor%芦笋清汁实用生产加工工艺

    Institute of Scientific and Technical Information of China (English)

    王德福; 汤小宁; 李保国; 张颖超

    2008-01-01

    芦笋营养价值较高,是高级保健食品;综合利用等级外芦笋和芦笋罐头、速冻芦笋生产中的下脚料,运用热烫、酶解寻工艺加工成色、香、味品质优良的高级芦笋清汁饮料,环保,经济,高附加值.工艺成熟,保证了产品的有机、保健,高档、优雅的品质.

  19. 二次回归旋转设计在石刁柏(Asparagus officinalis)细胞悬浮培养?…

    Institute of Scientific and Technical Information of China (English)

    周正来; 陆仕华

    1998-01-01

    应用二次回归旋转设计进行了石刁柏(Asparagusofficinalis)细胞悬浮培养条件的研究,试验因子的接种量(X1)和蔗糖(X2)谷氨酰胺(X3),肌醇(X4)和2,4-D(X5)的浓度,每因了设5水平,共32个处理,根据生长量建立石刁柏细胞悬浮培养生长函数模型,再进行电脑模拟试验求得最大生长量的优化因子水平组合(优化培养方案)最后实验验证结果表明,应用旋转设计方案培养的石刁柏细胞生长量比

  20. 长豇豆留种田采种适期研究%Studies on Fit Stage of Pick Seed of Asparagus Bean

    Institute of Scientific and Technical Information of China (English)

    李耀华; 胡志辉

    2000-01-01

    以之豇28-2为试材,研究了长豇豆(Vigna unguiculata ssp.Sesquipedalis(L.)Verd.)不同采种时期种子的活力及其田间生产性能.结果表明,不同采种时期种子的活力及田间生产性能有明显差异,长豇豆开花后18天采收的种子活力高、田间生产性能优良.因此,长豇豆留种田采种适期为开花后18d.

  1. Two New Iridoids from Verbena officinalis L.

    Directory of Open Access Journals (Sweden)

    Jicheng Shu

    2014-07-01

    Full Text Available Two new iridoids, 3-(5-(methoxycarbonyl-2-oxo-2H-pyran-3-ylbutanoic acid, named verbeofflin I (1, and 7-hydroxydehydrohastatoside (2, were isolated from the aerial part of Verbena officinalis L, along with three known iridoids, verbenalin (3, 3,4-dihydroverbenalin (4, hastatoside (5 by means of various column chromatography steps. The structures of these compounds were elucidated through analysis of their spectroscopic data obtained using 1D and 2D NMR and MS techniques. Verbeofflin I (1 is the new class of secoiridoid in the family Verbenaceae.

  2. DNA-based identification of Calendula officinalis (Asteraceae) 1

    OpenAIRE

    Schmiderer, Corinna; Lukas, Brigitte; Ruzicka, Joana; Novak, Johannes

    2015-01-01

    Premise of the study: For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples. Methods and Results: For this study, five markers (ITS, rbcL, 5′ trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and ...

  3. Inhibiton of photodynamic haemolysis by Gratiola officinalis L. extract

    Science.gov (United States)

    Tkachenko, Natalie; Pravdin, Alexander; Terentyuk, George; Navolokin, Nikita; Kurchatova, Maria; Polukonova, Natalia

    2015-03-01

    On the model of photodynamic haemolysis, the membranoprotective properties of a plant origin antioxidant, Gratiola officinalis L. extract, have been studied based on its ability to inhibit photodamage of sensitized erythrocyte membranes. The effect of different concentrations of the antioxidant on the photodynamic hemolysis has been studied; and the influence of incubation time on the membranoprotective properties of Gratiola officinalis L. extract has also been revealed.

  4. In vitro and ex vivo antiangiogenic activity of Salvia officinalis.

    Science.gov (United States)

    Keshavarz, Maryam; Mostafaie, Ali; Mansouri, Kamran; Bidmeshkipour, Ali; Motlagh, Hamid Reza Mohammadi; Parvaneh, Shahram

    2010-10-01

    Angiogenesis is a key process in the promotion of cancer and its metastasis. Herein, the antiangiogenic activity of Salvia officinalis extract and its fractions was investigated. S. officinalis aerial parts were extracted with ethanol and its successive hexane, ethyl acetate, n-butanol and aqueous fractions were evaluated for their antiangiogenic activities using human umbilical vein endothelial cells (HUVEC) capillary tube formation and rat aorta models in a three-dimensional collagen matrix. Furthermore, antimigrative effects of the fractions were assessed using a wound healing model. The ethanol extract of S. officinalis (ESO) potently inhibited capillary tube formation in HUVEC and rat aorta models of angiogenesis, and its hexane fraction (HSO) exerted the highest inhibitory effect. In addition, the ethanol extract of S. officinalis and its hexane fraction showed a dose-dependent inhibitory activity on the migration of the endothelial cells in the wound healing model. Furthermore, ESO inhibited endothelial cell proliferation at 50-200 μg/mL in a dose-dependent manner. These findings indicated some new pharmacological activities of S. officinalis such as antiangiogenic in vitro and ex vivo, and antimigrative activity in vitro. Therefore, S. officinalis could be a candidate as a useful herb with therapeutic or preventive activity against angiogenesis related disorders.

  5. The Effects of Medicinal Plants of Melissa officinalis and Salvia officinalis on Primary Dysmenorrhea

    Directory of Open Access Journals (Sweden)

    R. Kalvandi

    2014-07-01

    Full Text Available Introduction & Objective: Primary dysmenorrhea is one of the common problems in women, especially in young women. Although dysmenorrhea is not life threatening , it can have ad-verse effect on quality of life and cause disability or ineffectiveness. With regard to the young society suffering from it, various treatments are offered for dysmenorrhea one of which is medicinal plants. This study investigated the effect of Melissa officinalis and Salvia officinalis on primary dysmenorrhea Materials & Methods: This study was a clinical trial study performed on 50 girls with dys-menorrheal taking Salvia officinalis as infusion for five days before menstruation and five days during the menstruation. The consumption of the plants was repeated in the next two periods in the same way. Severity of pain, blooding duration and dysmenorrhea duration were evaluated with VAS (Visual Analog Scale Questionnaire in their next two cycles. Data were analyzed by chi-square, t-test and WILCOXON statistical test. Results: The results of this study showed that after using the plants, pain severity and pain duration significantly reduced P<0.01so that pain severity decreased from 6.30 to 3.94 and 3.24, (on a scale of 1 to 10 after taking the plants in the first and second periods ,respectively. The maximum pain duration before the treatment was 1 to 6 h (28.6% and this number reduced to less than 1 h after using these medicinal plants (38.0%. Mean of blooding duration was 6.36 days before the treatment. After the first and second duration, this value reached 6.48 and 6.34 days, respectively. It was determined that taking oral seda-tives reduced from 56% to 26% and 22% after the first and second periods, respectively. Fi-nally, after the second period, 42% of the girls reported high rates of satisfaction because of using the medicinal plants. Conclusion: Generally, it was showed that Melissa officinalis and Salvia officinalis signifi-cantly reduced pain severity and pain

  6. Diverse Belongings of Calendula officinalis: An Overview

    Directory of Open Access Journals (Sweden)

    M. U. Khan

    2011-07-01

    Full Text Available Herbal drugs therapy is regarded as an important therapy for the treatment of a wide range of diseases. However, enormous improvements have been observed in modern systems of medicine, but Indian herbal plants provide a rich source for health care to prevent different pathological states. Calendula officinalis is an aromatic, erect, annual herb that is indigenous to Europe, cultivated commonly in North America, Balkans, Eastern Europe, Germany and India. Ample studies have reported about the anti-inflammatory, antioxidant, antitumour, antigenotoxic, chemoprotective and hepatoprotective potential associated with the drug. The review article describes about the various phytochemical constituents associated with the plant extract. Moreover, numerous pleiotropic effects exhibited by the plant have been clearly discussed.

  7. [Comparative study on kidney tonifying and yang supporting effects of different processed products of Morinda officinalis].

    Science.gov (United States)

    Cui, N; Shi, Ji; Jia, Tian-Zhu

    2013-11-01

    To compare the pharmacological effects for tonifying kidney and supporting Yang of different processed products of Morinda officinalis. The oral administration of adenine was performed to establish the kidney-Yang deficiency mice model. Four different processed products, namely M. officinalis, morinda pulp, salt-steamed M. officinalis and licorice-processed M. officinalis, were administered, with Guifu Dihuang pills as the control drug, and mice signs, weight, testicular coefficient, seminal vesicle coefficient, serum testosterone, serum cortisol and serum creatinine as evaluation indexes. The results showed that all of the four processed products of M. officinalis could improve the symptoms of the kidney-Yang deficient mice. Among them, salt-steamed M. officinalis had the most significant effect, which was followed by licorice-processed M. officinalis, morinda pulp and M. officinalis. PMID:24558872

  8. Monoterpene synthases from common sage (Salvia officinalis)

    Energy Technology Data Exchange (ETDEWEB)

    Croteau, Rodney Bruce (Pullman, WA); Wise, Mitchell Lynn (Pullman, WA); Katahira, Eva Joy (Pullman, WA); Savage, Thomas Jonathan (Christchurch 5, NZ)

    1999-01-01

    cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.

  9. 几种麦冬及其伪品的组织学鉴别比较研究%Comparative Study on Histological Identification of Tuber of Dwarf Lilyturf and False Medical Material

    Institute of Scientific and Technical Information of China (English)

    刘义梅

    2005-01-01

    对麦冬的药典品种麦冬Ophiopogon japonicus(Thunb)Ker-Gawl,地方品种湖北麦冬Liriope spicata(Thunb.)Lour.var.proliferaY.T.Ma及阔叶麦冬Liriope platyphylla Wang et Tang,伪品淡竹根Lophatherum gracile Brongn.及石刁柏根Asparagus officinalis L.进行了组织学鉴别、显微摄影和特征比较等方面的研究.

  10. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Abdullah Ijaz Hussain

    2010-12-01

    Full Text Available The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%, camphor (17.1%, α-pinene (12.3%, limonene (6.23%, camphene (6.00% and linalool (5.70%. The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP and one fibroblast cell line (NIH-3T3 using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ and minimum inhibitory concentration (MIC of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  11. DNA-based identification of Calendula officinalis (Asteraceae)1

    Science.gov (United States)

    Schmiderer, Corinna; Lukas, Brigitte; Ruzicka, Joana; Novak, Johannes

    2015-01-01

    Premise of the study: For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples. Methods and Results: For this study, five markers (ITS, rbcL, 5′ trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and analyzed for species-specific mutations. With the application of two developed primer pairs located in the trnK 5′ intron and trnL-trnF, C. officinalis could be distinguished from other species of the genus and all outgroup samples tested. Adulterations of Calendula DNA in saffron could be detected down to 0.01%. Conclusions: With the developed assay, C. officinalis can be reliably identified and admixtures of this species as adulterant of saffron can be revealed at low levels. PMID:26649268

  12. Supercritical carbon dioxide extraction of antioxidants from rosemary (Rosmarinus officinalis L. and sage (Salvia officinalis L.

    Directory of Open Access Journals (Sweden)

    JASNA IVANOVIĆ

    2009-07-01

    Full Text Available The aim of the present study was to isolate and characterize antioxidant extracts obtained from dried leaves of rosemary (Rosmarinus officinalis L. and sage (Salvia officinalis L., originating from the southern Balkan Region. The antioxidant fraction was isolated from the plant material by supercritical carbon dioxide (SC-CO2 fractional extraction under a pressure of 30 MPa and at temperatures of 40 and 100 °C. In the present study, kinetic data and yields of antioxidant extracts obtained from dried leaves of rosemary and sage under different conditions were determined. Electron spin resonance (ESR spectroscopy assay on the ability of the extracts to scavenge stable 2,2-diphenyl-1-picrylhydrazyl (DPPH free radicals and reactive hydroxyl radicals during the Fenton reaction trapped by 5,5-dimethyl-1-pyrroline-N-oxide (DMPO showed that the investigated extracts had antioxidant activity comparable to that of butylated hydroxyanisole (BHA and commercial rosemary extract. The antioxidant fractions isolated at the higher temperature had higher antioxidant activities. A tentative analysis of the chemical composition of the antioxidant fractions obtained at the higher temperature was accomplished by LC-DAD and LC-MS analytical methods. Abietane-type diterpenoids, flavonoids and fatty acids were identified in the SC-CO2 extract of rosemary and sage.

  13. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Vantu Smaranda

    2015-11-01

    Full Text Available The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the samemedium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots weresubcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period

  14. Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

    Directory of Open Access Journals (Sweden)

    Aničić Nada V.

    2005-01-01

    Full Text Available The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%, neral (14.70% and citronellal (10.70%. The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity.

  15. Rosmarinic Acid and Melissa officinalis Extracts Differently Affect Glioblastoma Cells

    OpenAIRE

    Ramanauskiene, Kristina; Raudonis, Raimondas; Majiene, Daiva

    2016-01-01

    Lemon balm (Melissa officinalis L.) has many biological effects but especially important is its neuroprotective activity. The aim of the study is to produce different extracts of Melissa officinalis and analyse their chemical composition and biological properties on rat glioblastoma C6 cells. Results revealed that rosmarinic acid (RA) is the predominant compound of lemon balm extracts. RA has cytotoxic effect on glioblastoma cells (LC50 290.5 μM after the incubation of 24 h and LC50 171.3 μM ...

  16. Constituintes das sementes de Copaifera officinalis L. Constituents from Copaifera officinalis L. seeds

    Directory of Open Access Journals (Sweden)

    Valdir F. Veiga Junior

    2007-01-01

    Full Text Available O gênero Copaifera L. é um dos mais importantes economicamente na Região Amazônica devido, principalmente, à produção dos óleos de copaíba, oléo-resinas com diversas propriedades farmacológicas confirmadas. Apesar disso, os estudos fitoquímicos com as sementes das árvores do gênero Copaifera L.são raros. Copaifera officinalis foi a primeira espécie do gênero Copaifera a ser descrita. Este trabalho descreve a composição dos extratos obtidos em hexano e em acetato de etila das sementes de C. officinalis. No extrato obtido em hexano, a análise por cromatografia em fase gasosa utilizando padrões e através de espectrometria de massas permitiu a identificação de: esqualeno, tetradecano, hexadecano, campesterol, estigmasterol e beta-sitosterol; os ácidos graxos hexadecanóico, 9-octadecenóico e octadecanóico (majoritários; e decanóico, eicosanóico, docosanóico e tetracosanóico (minoritários. Cumarina foi isolada do extrato em acetato de etila e identificada por técnicas de RMN.Copaifera L. is one of the most economically important plant genera in the Amazon Region, since it exudes a resin-oil named copaiba oil possessing several confirmed pharmacological properties. In spite of that, phytochemical studies of the seeds from this genus are rare. Copaifera officinalis L. was the first species in the genus Copaifera to be described. This paper describes the chemical composition of the hexane and ethyl acetate extracts of the seeds from this species. In the hexane extract, gas chromatography and mass spectrometry analyses enabled us to identify squalene, tetradecane, hexadecane, campesterol, stigmasterol and beta-sitosterol; hexadecanoic, 9-octadecenoic and octadecanoic acids (major substances; as well as decanoic, eicosanoic, docosanoic and tetracosanoic (minor substances acids. Coumarin was detected in the ethyl acetate extract, isolated and identified by NMR.

  17. Hypersensitivity to Parietaria officinalis pollen in newcomers to the area with the plant.

    Science.gov (United States)

    Cvitanović, S; Marusić, M; Juricić, M; Vrdoljak, E; Petrovecki, M; Rozga, A; Stavljenić-Rukavina, A

    1993-11-01

    Hypersensitivity to Parietaria officinalis (wall pellitory) pollen and other environmental allergens was studied in pollinosis patients allergic to P. officinalis pollen who were born in areas without P. officinalis and later moved to the city of Split, where P. officinalis is responsible for some 65% of pollinosis cases. Highly significant positive correlations were found for both the intensity of skin test reaction and concentration of specific serum IgE with the length of residence in the area. In contrast, the respective data on subjects hypersensitive to P. officinalis pollen allergen, but born and living in the area of Split, revealed a tendency to negative correlation between age and intensity of hypersensitivity to P. officinalis. A number of patients from both groups were tested for presence of serum IgE antibodies specific for 14 common environmental allergens. Hypersensitivity to P. officinalis pollen was associated with hypersensitivity to olive, mugwort, and birch pollen in newcomers; hypersensitivity to birch and, to some extent, olive pollen was significantly more frequent in newcomers than in autochthonous patients who were allergic to P. officinalis pollen. Regardless of whether the patients were autochthons or newcomers to the area with P. officinalis, hypersensitivity to P. officinalis mostly excluded hypersensitivity to Dermatophagoides farinae and D. pteronyssinus, and vice versa.

  18. Two New Triterpenoids from the Roots of Sanguisorba officinalis L.

    Institute of Scientific and Technical Information of China (English)

    Fan ZHANG; Tie-Jun FU; Shu-Lin PENG; Zhong-Rong LIU; Li-Sheng DING

    2005-01-01

    Two new triterpenoids, octanordammar- 1,11,13(17)-trien- 17-ol-3,16-dione (1) and lup- 12-en- 15α,19β-diol-3,11-dioxo-28-oic acid (4), as well as 13 known compounds were isolated from the roots of Sanguisorba officinalis L. (Rosaceae). Their structures were determined using spectroscopic methods.

  19. Methyl carnosate, an antibacterial diterpene isolated from Salvia officinalis leaves.

    Science.gov (United States)

    Climati, Elisa; Mastrogiovanni, Fabio; Valeri, Maria; Salvini, Laura; Bonechi, Claudia; Mamadalieva, Nilufar Zokirzhonovna; Egamberdieva, Dilfuza; Taddei, Anna Rita; Tiezzi, Antonio

    2013-04-01

    Ethanolic extracts of Salvia officinalis leaves demonstrated antibacterial activity against Bacillus cereus. Fractionation of the extracts led to the isolation of the most active antibacterial compound, which, from spectroscopic and LC-MS evidence, was proved to be the diterpene, methyl carnosate.

  20. Seed germination of Calendula officinalis 'Carola' in response to temperature

    Science.gov (United States)

    Calendula (Calendula officinalis 'Carola') is a potential agronomic oilseed crop with application in the paint, coating, and cosmetic industry. Calendula has historically been used for herbal medicinal purposes and an ornamental plant. With the discovery that calendula seeds contain high concentrati...