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Sample records for artiss fibrin sealant

  1. Role of Fibrin Sealants in Liver Surgery

    NARCIS (Netherlands)

    de Boer, Marieke T.; Boonstra, Elizabeth A.; Lisman, Ton; Porte, Robert J.

    2012-01-01

    Background: Fibrin sealants are widely used in liver surgery. The aim of this article is to review the literature on evidence of hemostatic and biliostatic capacities of different fibrin sealants in liver surgery. Methods: In PubMed, a literature search was done with the search terms 'fibrin sealant

  2. Fibrin sealant for mesh fixation in laparoscopic umbilical hernia repair

    DEFF Research Database (Denmark)

    Eriksen, J R; Bisgaard, T; Assaadzadeh, S;

    2013-01-01

    Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown.......Fibrin sealant for mesh fixation has significant positive effects on early outcome after laparoscopic ventral hernia repair (LVHR) compared with titanium tacks. Whether fibrin sealant fixation also results in better long-term outcome is unknown....

  3. A Review of Bioceramics and Fibrin Sealant

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    Le Guéhennec L.

    2004-09-01

    Full Text Available This review focuses on bone substitute composites made by mixing ceramic biomaterials with fibrin sealants. Different biomaterials such as coral, bone-derived materials, bioactive glass ceramics, and synthetic calcium phosphate have been mixed with fibrin sealant, resulting in a combination of the biological properties of the two components. This type of association has not produced identical results in all studies. In the past for some, the addition of fibrin sealant to the biomaterial failed to produce any significant, positive effect on osteointegration, whereas others found a positive impact on bone colonization. Despite the negative biological effects reported previously, bioceramic-fibrin composites have been widely used in various types of bone surgery because they are easy to manipulate. In particular, the intra-operative preparation of these composites makes it possible to add bone growth factors or autologous osteoprogenitor cells prior to bone reconstruction. The bone growth factors and autologous osteoprogenitor cells associated with the bioceramic-fibrin composites should provide surgeons with tissue engineered grafts with enhanced osteointegrative properties. This review discusses both the advantages and disadvantages, as well as the future perspectives, of using bioceramic-fibrin composites in various clinical indications.

  4. Fibrin sealant use in pilonidal sinus: Systematic review

    Institute of Scientific and Technical Information of China (English)

    Cuneyt Kayaalp; Ismail Ertugrul; Kerem Tolan; Fatih Sumer

    2016-01-01

    AIM: To review the current data about the success rates of fibrin sealant use in pilonidal disease.METHODS: Fibrin sealant can be used for different purposes in pilonidal sinus treatment, such as filling in the sinus tracts, covering the open wound after excision and lay-open treatment, or obliterating the subcutaneous dead space before skin closure. We searched Pubmed, Google-Scholar, Ebsco-Host, clinicaltrials, and Cochrane databases and found nine studies eligible for analysis; these studies included a total of 217 patients(84% male, mean age 24.2 ± 7.8). RESULTS: In cases where fibrin sealant was used to obliterate the subcutaneous dead space, there was no reduction in wound complication rates(9.8% vs 14.6%, P = 0.48). In cases where sealant was used to cover the laid-open area, the wound healing time and patient comfort were reported better than in previous studies(mean 17 d, 88% satisfaction). When fibrin sealant was used to fill the sinus tracts, the recurrence rate was around 20%, despite the highly selected grouping of patients.CONCLUSION: Consequently, using fibrin sealant to decrease the risk of seroma formation was determined to be an ineffective course of action. It was not advisable to fill the sinus tracts with fibrin sealant because it was not superior to other cost-effective and minimally invasive treatments. New comparative studies can be conducted to confirm the results of sealant use in covering the laid-open area.

  5. Fibrin sealant in general surgery. Personal experience and literary review.

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    Gubitosi, Adelmo; Ruggiero, Roberto; Docimo, Giovanni; Esposito, Alessandro

    2014-01-01

    In consideration of the use of fibrin glue in a general surgery department, authors analyze their last two years series. Operations on liver and biliary ducts, bowel and proctologic surgery, thyroid and breast surgery, abdominal wall hernias, fistulas and difficult wounds are considered with a literary review on fibrin sealant.

  6. Aulogous fibrin sealant (Vivostat ® in the neurosurgical practice: Part II: Vertebro-spinal procedures

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    Francesca Graziano

    2016-01-01

    Conclusions: In this preliminary study, the application of autologous fibrin sealant with Vivostat ® resulted in rapid hemostasis and/or acted as an effective dural sealant. Although this product appears to be safe and effective, further investigations are warranted.

  7. Venous gas embolism caused by fibrin sealant application to the prostate during greenlight laser photoselective vaporization.

    Science.gov (United States)

    Lee, Alexander; Vazquez, Rafael

    2015-04-15

    Venous gas embolism is a complication of fibrin sealant application and is a well-described event during various modes of prostate resection. We describe the case of a nitrogen venous gas embolism during Greenlight laser photovaporization of the prostate during the application of fibrin sealant to the operative site for hemostasis. Fibrin sealant application by a compressed gas applicator is a cause of venous air embolism, and this case highlights the need to keep venous gas embolism in mind when compressed gas applicators are used.

  8. Does Fibrin Sealant Reduce Seroma after Immediate Breast Reconstruction Utilizing a Latissimus Dorsi Myocutaneous Flap?

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    Han Gyu Cha

    2012-09-01

    Full Text Available Background The most common complication of latissimus dorsi myocutaneous flap in breastreconstruction is seroma formation in the back. Many clinical studies have shown that fibrinsealant reduces seroma formation. We investigated any statistically significant differences inpostoperative drainage and seroma formation when utilizing the fibrin sealant on the site ofthe latissimus dorsi myocutaneous flap harvested for immediate breast reconstruction afterskin-sparing partial mastectomy.Methods A total of 46 patients underwent immediate breast reconstruction utilizing alatissimus dorsi myocutaneous island flap. Of those, 23 patients underwent the procedurewithout fibrin sealant and the other 23 were administered the fibrin sealant. All flaps wereelevated with manual dissection by the same surgeon and were analyzed to evaluate thepotential benefits of the fibrin sealant. The correlation analysis and Mann-Whitney U testwere used for analyzing the drainage volume according to age, weight of the breast specimen,and body mass index.Results Although not statistically significant, the cumulative drainage fluid volume was higherin the control group until postoperative day 2 (530.1 mL compared to 502.3 mL, but thefibrin sealant group showed more drainage beginning on postoperative day 3. The donor sitecomparisons showed the fibrin sealant group had more drainage beginning on postoperativeday 3 and the drain was removed 1 day earlier in the control group.Conclusions The use of fibrin sealant resulted in no reduction of seroma formation. Becausethe benefits of the fibrin sealant are not clear, the use of fibrin sealant must be fully discussedwith patients before its use as a part of informed consent.

  9. Lower reoperation rates with the use of fibrin sealant versus tacks for mesh fixation

    DEFF Research Database (Denmark)

    Helvind, Neel Maria; Andresen, Kristoffer; Rosenberg, Jacob

    2013-01-01

    BACKGROUND: Groin hernia repair may be associated with long-term complications such as chronic pain, believed to result from damage to regional nerves by tissue penetrating mesh fixation. Studies have shown that mesh fixation with fibrin sealant reduces the risk of these long-term complications......, but data on recurrence and reoperation rates after the use of fibrin sealant compared with tacks are not available. This study aimed to determine whether fibrin sealant is a safe and feasible alternative to tacks with regard to reoperation rates after laparoscopic groin hernia repair. METHODS: The current...... study compared reoperation rates after laparoscopic groin hernia repair between fibrin sealant and tacks used for mesh fixation. The study used data collected prospectively from The National Danish Hernia Database and analyzed 8,314 laparoscopic groin hernia repairs for reoperation rates. Mesh fixation...

  10. High-Pressure Fibrin Sealant Foam: An Effective Hemostatic Agent for Treating Severe Parenchymal Hemorrhage

    Science.gov (United States)

    2008-01-01

    compression or vascular control. Materials and methods. FSF with high fibrinogen concentration (20 mg/mL) and low thrombin activity (5 U/mL) was prepared and...fibrin sealant foam (FSF) into a body cavity via trocar would spread throughout the cavity, bind to damaged tissue, and stop bleeding from inaccessible...liver hemorrhage model in anticoagulated rabbits. We then compared pressurized FSF with liq- uid and a powdered form of fibrin sealant. MATERIALS AND

  11. Taurolidine-Fibrin-Sealant-Matrix using spray application for local treatment of brain tumors.

    Science.gov (United States)

    Stendel, Ruediger; Scheurer, Louis; Schlatterer, Kathrin; Gminski, Richard; Möhler, Hanns

    2004-01-01

    Malignant gliomas tend to recur in the vast majority of cases. Recurrent gliomas may arise from vital tumor cells present in this zone around the resection margin. It appears promising to combine tumor resection with local chemotherapy using an antineoplastic, but non-toxic agent. Taurolidine exerts a selective antineoplastic effect by induction of programmed cell death and has anti-angiogenic activity. Fibrin sealant is completely degradable and firmly adheres to brain tissue, suggesting that it would provide a suitable matrix for taurolidine delivery--a Taurolidine-Fibrin-Sealant-Matrix (TFM)--in the local treatment of brain tumors. The potential of local delivery of taurolidine out of a fibrin sealant matrix was investigated. Taurolidine could be suspended homogeneously in both the thrombin and the procoagulant protein components of the fibrin sealant. The fibrin sealant matrix was a suitable carrier for the suspension of taurolidine at a concentration that ensured the release of therapeutically effective amounts of the drug over a period of 2 weeks in vitro. The antineoplastic action of taurolidine was not affected by embedding in the fibrin sealant matrix. The described drug delivery system may be suitable for local taurolidine treatment of brain tumors following complete or partial resection or of tumors that are non-resectable because of their location.

  12. Application of Fibrin Glue Sealant After Hepatectomy Does Not Seem Justified

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    Figueras, Juan; Llado, Laura; Miro, Mónica; Ramos, Emilio; Torras, Jaume; Fabregat, Juan; Serrano, Teresa

    2007-01-01

    Objective: To evaluate the efficacy, amount of hemorrhage, biliary leakage, complications, and postoperative evolution after fibrin glue sealant application in patients undergoing liver resection. Summary Background Data: Fibrin sealants have become popular as a means of improving perioperative hemostasis and reducing biliary leakage after liver surgery. However, trials regarding its use in liver surgery remain limited and of poor methodologic quality. Patients and Methods: A total of 300 patients undergoing hepatic resection were randomly assigned to fibrin glue application or control groups. Characteristics and debit of drainage and postoperative complications were evaluated. The amount of blood loss, measurements of hematologic parameters liver test, and postoperative evolution (particularly involving biliary fistula and morbidity) was also recorded. Results: Postoperatively, no differences were observed in the amount of transfusion (0.15 ± 0.66 vs. 0.17 ± 0.63 PRCU; P = 0.7234) or in the patients that required transfusion (18% vs. 12%; P = 0.2), respectively, for the fibrin glue or control group. There were no differences in overall drainage volumes (1180 ± 2528 vs. 960 ± 1253 mL) or in days of postoperative drainage (7.9 ± 5 vs. 7.1 ± 4.7). Incidence of biliary fistula was similar in the fibrin glue and control groups, (10% vs. 11%). There were no differences regarding postoperative morbidity between groups (23% vs. 23%; P = 1). Conclusions: Application of fibrin sealant in the raw surface of the liver does not seem justified. Blood loss, transfusion, incidence of biliary fistula, and outcome are comparable to patients without fibrin glue. Therefore, discontinuation of routine use of fibrin sealant would result in significant cost saving. PMID:17414601

  13. Safety and efficacy of a novel, dry powder fibrin sealant for hemostasis in hepatic resection.

    NARCIS (Netherlands)

    Ruitenbeek, K.; Ayez, N.; Verhoef, C.; Wilt, J.H.W. de; Bottema, J.; Rijken, A.M.; Rij, M. van; Koopman, J; Zuckerman, L.A.; Frohna, P.; Porte, R.J.

    2014-01-01

    BACKGROUND/AIMS: Fibrocaps is a dry powder fibrin sealant containing human plasma-derived fibrinogen and thrombin. The safety, efficacy, and application methods for Fibrocaps were evaluated in an exploratory, first-in-human, noncomparative, clinical study. METHODS: Patients with minor bleeding/oozin

  14. Safety and Efficacy of a Novel, Dry Powder Fibrin Sealant for Hemostasis in Hepatic Resection

    NARCIS (Netherlands)

    Ruitenbeek, Karin; Ayez, N.; Verhoef, C.; de Wilt, J. Hans W.; Bottema, Jan; Rijken, Arjen M.; van Rij, Monique; Koopman, Jaap; Zuckerman, Linda A.; Frohna, Paul; Porte, Robert J.

    2014-01-01

    Background/Aims: Fibrocaps is a dry powder fibrin sealant containing human plasma-derived fibrinogen and thrombin. The safety, efficacy, and application methods for Fibrocaps were evaluated in an exploratory, first-in-human, non-comparative, clinical study. Methods: Patients with minor bleeding/oozi

  15. Long-Standing Motor and Sensory Recovery following Acute Fibrin Sealant Based Neonatal Sciatic Nerve Repair

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    Natalia Perussi Biscola

    2016-01-01

    Full Text Available Brachial plexus lesion results in loss of motor and sensory function, being more harmful in the neonate. Therefore, this study evaluated neuroprotection and regeneration after neonatal peripheral nerve coaptation with fibrin sealant. Thus, P2 neonatal Lewis rats were divided into three groups: AX: sciatic nerve axotomy (SNA without treatment; AX+FS: SNA followed by end-to-end coaptation with fibrin sealant derived from snake venom; AX+CFS: SNA followed by end-to-end coaptation with commercial fibrin sealant. Results were analyzed 4, 8, and 12 weeks after lesion. Astrogliosis, microglial reaction, and synapse preservation were evaluated by immunohistochemistry. Neuronal survival, axonal regeneration, and ultrastructural changes at ventral spinal cord were also investigated. Sensory-motor recovery was behaviorally studied. Coaptation preserved synaptic covering on lesioned motoneurons and led to neuronal survival. Reactive gliosis and microglial reaction decreased in the same groups (AX+FS, AX+CFS at 4 weeks. Regarding axonal regeneration, coaptation allowed recovery of greater number of myelinated fibers, with improved morphometric parameters. Preservation of inhibitory synaptic terminals was accompanied by significant improvement in the motor as well as in the nociceptive recovery. Overall, the present data suggest that acute repair of neonatal peripheral nerves with fibrin sealant results in neuroprotection and regeneration of motor and sensory axons.

  16. Effect of implanting fibrin sealant with ropivacaine on pain after laparoscopic cholecystectomy

    Institute of Scientific and Technical Information of China (English)

    Jian-Zhu Fu; Jie Li; Ze-Li Yu

    2009-01-01

    AIM: To investigate the safety and efficacy of implanting fibrin sealant with sustained-release ropivacaine in the gallbladder bed for pain after laparoscopic cholecystectomy (LC). METHODS: Sixty patients (American Society of Anesthesiologists physical status was Ⅰ or Ⅱ and underwent LC) were randomly divided into three equal groups: group A (implantation of fibrin sealant in the gallbladder bed), group B (implantation of fibrin sealant carrying ropivacaine in the gallbladder bed), and group C (normal saline in the gallbladder bed). Postoperative pain was evaluated, and pain relief was assessed by visual analog scale (VAS) scoring. RESULTS: The findings showed that 81.7% of patients had visceral pain, 50% experienced parietal, and 26.7% reported shoulder pain after LC. Visceral pain was significantly less in group B patients than in the other groups ( P < 0.05), and only one patient in this group experienced shoulder pain. The mean VAS score in group B patients was lower than that in the other groups. CONCLUSION: Visceral pain is prominent after LC and can be effectively controlled by implanting fibrin sealant combined with ropivacaine in the gallbladder bed.

  17. Fibrin sealant for mesh fixation in laparoscopic groin hernia repair does not increase long-term recurrence

    DEFF Research Database (Denmark)

    Fenger, Andreas Qwist; Helvind, Neel Maria; Pommergaard, Hans-Christian;

    2016-01-01

    ) laparoscopic groin hernia repair using either fibrin sealant or tacks for mesh fixation. METHODS: This study used data from the Danish Hernia Database to create the following cohort: All patients operated laparoscopically for primary groin hernia with a TAPP procedure using fibrin sealant for mesh fixation....... These patients were matched 1:2 with patients, where the mesh was fixated using tacks. A validated questionnaire was sent to all included patients to determine recurrence, which was defined as reoperation or clinical diagnosis of recurrence by a physician. Follow-up was from index operation to either reoperation...... were found, of which 30 (5.8%) were in the fibrin sealant group and 84 (8.3%) in the tacks group (p = 0.084). The Cox regression analysis found no difference in recurrence with the use of tacks compared to fibrin sealant (hazard ratio 0.8) [95% CI (0.5-1.2)]. CONCLUSION: We found no significant...

  18. Treatment of post-prostatectomy rectourethral fistula with fibrin sealant (Quixil™) injection: a novel application.

    Science.gov (United States)

    Verriello, V; Altomare, M; Masiello, G; Curatolo, C; Balacco, G; Altomare, D F

    2010-12-01

    Rectourethral fistulas in adults is a rare but potentially devastating postoperative condition requiring complex and demanding surgery. Fibrin glue treatment has been used with some success in anal and rectovaginal fistulas, and in the case we present here this indication has been extended to a postoperative rectourethral fistula following radical prostatectomy. For the first time, to our knowledge, a fibrin sealant (Quixil) was injected into the fistula tract, and a rectal mucosal flap was used to close the internal opening. The fistula healed in few weeks, and the patient is symptom free after 1 year of follow-up.

  19. Fibrin Sealant for Prevention of Resection Surface-Related Complications After Liver Resection A Randomized Controlled Trial

    NARCIS (Netherlands)

    de Boer, Marieke T.; Klaase, Joost M.; Verhoef, Cornelis; van Dam, Ronald M.; van Gulik, Thomas M.; Molenaar, I. Quintus; Bosscha, Koop; Dejong, Cornelis H. C.; Van der Jagt, Eric J.; Porte, Robert J.

    2012-01-01

    Objective: To evaluate the efficacy of fibrin sealant in reducing resection surface-related complications in liver surgery. Background: Bile leakage, bleeding, and abscess formation are major resection surface-related complications after liver resection. It is unclear whether application of fibrin s

  20. Fibrin Sealant Foam Sprayed Directly on Liver Injuries Decreases Blood Loss in Resuscitated Rats

    Science.gov (United States)

    2000-08-01

    based on the dry fibrin sealant dressing concept. This material could potentially be intro- duced into a body cavity by a trocar , spread throughout...Special Operations Command, Biomedical Initiatives Steering Committee. The foam material and funding for a research technician was provided by the...was performed to evaluate the effectiveness of FSF when sprayed directly on a severe liver injury in rats. MATERIALS AND METHODS Thirty-six Sprague

  1. Comparison of commercial fibrin sealants in facelift surgery: a prospective study

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    Botti G

    2013-11-01

    Full Text Available Giovanni Botti,1 Michele Pascali,2 Chiara Botti,1 Florian Bodog,3 Pietro Gentile,2 Valerio Cervelli2 1Villa Bella Clinic, Salò, 2Department of Plastic and Reconstructive Surgery, University of Rome Tor Vergata, Italy; 3University of Oradea, Oradea, Romania Background: The aim of this study was to compare the effects of two types of fibrin glue in patients undergoing facelift surgery. Methods: A prospective, controlled "right-left side" study was carried out in 20 patients. The two fibrin sealants used were Quixil® and Tissucol®. The two sealants were used at the same time, ie, one on one side of the face and the other on the contralateral side. Comparisons were made with regard to rates of hematoma and seroma, degree of induration, edema, ecchymosis, pain levels, and patient satisfaction. Results: The results were almost equivalent. The only exception was a significant (40 mL hematoma in a patient treated with Quixil. Bleeding was most likely due to a sudden rise in blood pressure during the immediate postoperative period. However, it must be emphasized that, while Tissucol actually seals the undermined area, thus virtually eliminating the dead space, Quixil acts differently, in that its effectiveness in preventing hematoma is linked mainly to its hemostatic effect. Conclusion: The two fibrin sealants used were nearly identical with regard to patient safety and quality of the result. Nevertheless, it is noted that, while Tissucol has both hemostatic and "gluing" effects, Quixil is mainly effective in securing hemostasis. Keywords: facelift surgery, rhytidectomy, fibrin sealants, hematoma

  2. Treatment of venous ulcers with fibrin sealant derived from snake venom

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    MAN Gatti

    2011-01-01

    Full Text Available Venous ulcers of the lower limbs complicated by infection or chronicity represent a serious public health problem. The elevated number of those afflicted burdens the health services, interferes in quality of life and causes absenteeism. Although there are 2,500 items on the market, ranging from the simplest dressing up to the most complex types of dressing, treatment remains a challenge. Among the substances used, fibrin sealant is the one that promotes diminution of bacterial colonization and of edema, controls hemorrhaging, alters the pain threshold by protecting the nerve endings, hydrates the wound bed and forms granulation tissue that favors healing. Its disadvantages include higher cost and utilization of human fibrinogen that can transmit infectious diseases. The Center for the Study of Venoms and Venomous Animals (CEVAP at São Paulo State University (UNESP developed a new sealant made up of fibrinogen extracted from large animals and from an enzyme obtained from snake venom. The present study, developed in the Health Education Clinic (CEPS of Sacred Heart University (USC aimed to evaluate the effect of the new sealant on the healing process of venous ulcers in 24 adult patients, seven of whom were male and 17 female. Two study groups were formed as follows: Group 1 (G1 - control group of 11 patients treated with essential fatty acid (EFA and Unna's boot, and Group 2 (G2 - 13 patients treated with essential fatty acid (EFA, fibrin sealant and Unna's boot. The follow-up lasted eight weeks and the sealant was applied at only the first and fourth weeks. The results showed that Group 2 presented worse lesion conditions as to healing, but, when comparing the two groups, it was noteworthy that the the sealant was effective in healing venous ulcers. There is evidence that the new sealant is recommended for leg ulcers with the following advantages: ease of application, preparation of the wound bed, diminution of pain and a higher number of

  3. Randomized clinical trial of fibrin sealant versus titanium tacks for mesh fixation in laparoscopic umbilical hernia repair

    DEFF Research Database (Denmark)

    Eriksen, J R; Bisgaard, T; Assaadzadeh, S;

    2011-01-01

    The use of tacks for mesh fixation may induce pain after surgery for ventral hernia. The aim of this study was to compare postoperative pain after laparoscopic ventral hernia repair (LVHR) with conventional mesh fixation using titanium tacks versus fibrin sealant (FS).......The use of tacks for mesh fixation may induce pain after surgery for ventral hernia. The aim of this study was to compare postoperative pain after laparoscopic ventral hernia repair (LVHR) with conventional mesh fixation using titanium tacks versus fibrin sealant (FS)....

  4. Anaphylactic reaction after systemic application of aprotinin triggered by aprotinin-containing fibrin sealant.

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    Kober, Benjamin J; Scheule, Albertus M; Voth, Vladimir; Deschner, Norbert; Schmid, Eckhard; Ziemer, Gerhard

    2008-08-01

    We report a 67-yr-old male after multiple surgical procedures for treatment of arterial occlusive disease who suffered an anaphylactic reaction after administration of aprotinin (Trasylol) prior to urgent coronary artery bypass surgery. The patient had been treated with aprotinin-containing fibrin sealant in 2004 and in 2007, 2 wk before coronary artery bypass surgery. The postoperative serologic screening revealed positive results for qualitative aprotinin-specific IgG, highly elevated quantitative aprotinin-specific IgG and moderately elevated aprotinin-specific IgE antibodies.

  5. A new heterologous fibrin sealant as a scaffold to cartilage repair-Experimental study and preliminary results.

    Science.gov (United States)

    de Barros, Caio Nunes; Miluzzi Yamada, Ana Lúcia; Junior, Rui Seabra F; Barraviera, Benedito; Hussni, Carlos Alberto; de Souza, Jaqueline Brandão; Watanabe, Marcos Jun; Rodrigues, Celso Antônio; Garcia Alves, Ana Liz

    2016-07-01

    Autologous fibrin gel is commonly used as a scaffold for filling defects in articular cartilage. This biomaterial can also be used as a sealant to control small hemorrhages and is especially helpful in situations where tissue reparation capacity is limited. In particular, fibrin can act as a scaffold for various cell types because it can accommodate cell migration, differentiation, and proliferation. Despite knowledge of the advantages of this biomaterial and mastery of the techniques required for its application, the durability of several types of sealant at the site of injury remains questionable. Due to the importance of such data for evaluating the quality and efficiency of fibrin gel formulations on its use as a scaffold, this study sought to analyze the heterologous fibrin sealant developed from the venom of Crotalus durissus terrificus using studies in ovine experimental models. The fibrin gel developed from the venom of this snake was shown to act as a safe, stable, and durable scaffold for up to seven days, without causing adverse side effects. Fibrin gel produced from the venom of the Crotalus durissus terrificus snake possesses many clinical and surgical uses. It presents the potential to be used as a biomaterial to help repair skin lesions or control bleeding, and it may also be used as a scaffold when applied together with various cell types. The intralesional use of the fibrin gel from the venom of this snake may improve surgical and clinical treatments in addition to being inexpensive and adequately consistent, durable, and stable. The new heterologous fibrin sealant is a scaffold candidate to cartilage repair in this study.

  6. Staged Mucosal Advancement Flap versus Staged Fibrin Sealant in the Treatment of Complex Perianal Fistulas

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    S. J. van der Hagen

    2011-01-01

    Methods. All patients with high complex cryptoglandular fistulas were randomised to closure of the internal opening by a mucosal advancement flap (MF or injection with fibrin sealant (FS after treatment with setons. Recurrence rate and incontinence disorders were explored. Results. The MF group (5 females and 10 males with a median age of 51 years and a median followup of 52 months. The FS group (4 females and 11 males with a median age of 45 years and a median followup of 49 months. Three (20% patients of the MF group had a recurrent fistula compared to 9 (60% of the FS group (P=0.03. No new continence disorders developed. Conclusion. Staged FS injection has a much lower success rate compared to MF.

  7. Characterization of mechanics and cytocompatibility of fibrin-genipin annulus fibrosus sealant with the addition of cell adhesion molecules.

    Science.gov (United States)

    Guterl, Clare C; Torre, Olivia M; Purmessur, Devina; Dave, Khyati; Likhitpanichkul, Morakot; Hecht, Andrew C; Nicoll, Steven B; Iatridis, James C

    2014-09-01

    There is an unmet clinical need for a biomaterial sealant capable of repairing small annulus fibrosus (AF) defects. Causes of these defects include painful intervertebral disc herniations, microdiscectomy procedures, morbidity associated with needle puncture injury from discography, and future nucleus replacement procedures. This study describes the enhancements of a fibrin gel through genipin crosslinking (FibGen) and the addition of the cell adhesion molecules (CAMs), fibronectin and collagen. The gel's performance as a potential AF sealant is assessed using a series of in vitro tests. FibGen gels with CAMs had equivalent adhesive strength, gene expression, cytomorphology, and cell proliferation as fibrin alone. However, FibGen gels had enhanced material behaviors that were tunable to higher shear stiffness values and approximated human annulus tissue as compared with fibrin alone, were more dimensionally stable, and had a slower in vitro degradation rate. Cytomorphology of human AF cells cultured on FibGen gels exhibited increased elongation compared with fibrin alone, and the addition of CAMs to FibGen did not significantly affect elongation. This FibGen gel offers the promise of being used as a sealant material to repair small AF defects or to be used in combination with other biomaterials as an adhesive for larger defects.

  8. Application of fibrin sealant at the urethrovesical anastomosis in robotic assisted radical prostatectomy: does it enable earlier Foley catheter and Jackson-Pratt drain removal?

    Science.gov (United States)

    Flury, Sarah C; Starnes, Danielle N; Steers, William D

    2008-01-01

    Leakage at the urethrovesical anastomosis in the post-operative period can result in morbidity including ileus. We examined the effectiveness of using a fibrin sealant at the anastomosis to limit urine leakage thereby facilitating earlier Jackson-Pratt drain and Foley catheter removal following robotic assisted laparoscopic prostatectomy (RALRP). Forty consecutive patients underwent RALRP by one surgeon at our institution. The first 20 patients underwent standard operation and served as the control group. The subsequent 20 patients underwent the same operation with addition of fibrin sealant following a running absorbable sutured urethrovesical anastomosis. The two groups were compared for age (60.5 vs. 58.2 years), pre-operative PSA (5.23 vs. 4.71), Gleason score (6.3 vs. 6.5), stage at resection, and prostate size at resection (51.7 vs. 47.7 g). Wilcoxon rank sum test determined no statistically significant differences in the groups. Patients in the fibrin sealant group had 1.3 versus 2.1 days with a Jackson-Pratt drain, 9.75 versus 12.1 days with a catheter, and an average of 38.6 versus 63.2 cc of drainage per shift. Catheters were removed when a cystogram demonstrated no extravasation of contrast. Two patients in the control group and no patients in the fibrin sealant group had large-volume leakage and ileus post-operatively. In patients undergoing RALRP, application of fibrin sealant at the urethrovesical anastomosis appears to facilitate sealing, thereby allowing earlier removal of the JP drain, by 0.8 days, and the Foley catheter, by 2.35 days, than in controls. No patients in the fibrin sealant group suffered post-operative ileus. This adjunct may be especially useful early in the learning process to reduce morbidity.

  9. Fibrin sealant does not decrease seroma output or time to drain removal following inguino-femoral lymph node dissection in melanoma patients: A randomized controlled trial (NCT00506311

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    Mansfield Paul F

    2008-06-01

    Full Text Available Abstract Background This study assessed the impact of closed suction drains and evaluated whether the intraoperative use of a fibrin sealant decreased time to drain removal and wound complications in melanoma patients undergoing inguino-femoral lymph node dissection. Methods A pilot study (n = 18 assessed the impact of a closed suction drain following inguino-femoral lymph node dissection. A single-institution, prospective trial was then performed in which patients were randomized to a group that received intraoperative application of a fibrin sealant following inguino-femoral lymph node dissection or to a control group that did not receive sealant. Results The majority of the patients enrolled felt the drains caused moderate or severe discomfort and difficulties with activities of daily living. Thirty patients were then randomized; the median time to drain removal in the control group (n = 14 was 30 days (range, 13–74 compared to 29 days (range, 11–45 in the fibrin sealant group (n = 16; P = 0.6. Major and minor complications were similar in the two groups. Conclusion Postoperative closed suction drains were associated with major patient inconvenience. Applying a fibrin sealant at the time of inguino-femoral lymph node dissection in melanoma patients did not reduce the time to drain removal or postoperative morbidity. Alternative strategies are needed.

  10. No difference in sexual dysfunction after transabdominal preperitoneal (TAPP) approach for inguinal hernia with fibrin sealant or tacks for mesh fixation

    DEFF Research Database (Denmark)

    Pommergaard, Hans Christian; Burcharth, J; Andresen, K;

    2016-01-01

    BACKGROUND: Postoperative sexual dysfunction in relation to laparoscopic groin hernia surgery may be related to methods of mesh fixation. However, this has not been investigated earlier. Moreover, results regarding sexual dysfunction in females have not been reported systematically. The aim...... of this study was to compare fibrin sealant versus tacks for fixation of mesh regarding sexual dysfunction in males and females. METHODS: Using the Danish Hernia Database, patients operated laparoscopically for groin hernia with a transabdominal preperitoneal (TAPP) procedure with fibrin sealant or tacks...

  11. Transplantation of neonatal cardiomyocytes plus fibrin sealant restores myocardial function in a rat model of myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    LI Yong-shun; GAO Bing-ren

    2007-01-01

    Background Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myocardial infarction.Methods The left anterior descending artery in adult female Sprague-Dawley (SD) rats was ligated to make a myocardial infarction model. Neonatal ventricular cardiomyocytes from one-day male SD rats were isolated, labeled and cultured. The cells were injected into the infarcted area three weeks later. The animals were randomized into four recipient groups: (1) cardiomyocytes plus fibrin sealant (group CF, n=10); (2) cardiomyocytes alone (group C, n=10); (3)fibrin sealant recipients alone (group F, n=10); (4) control group (n=10). Four weeks after transplantation,echocardiography and Langerdoff model were used to assess heart function. Immunohistochemical staining and polymerase chain reaction (PCR) were performed to track the implanted cardiomyocytes and detect the sex-determining region Y gene on Y chromosome.Results Echocardiography showed the fraction shortening (FS) in groups CF, C, F and control group was (27.80±6.32)%, (22.29±4.54)%, (19.24±6.29)% and (20.36±3.29)% respectively with statistically significant differences in group CF compared with the other groups (P<0.05). The Langendoff model revealed that the left ventricular development of peak pressure (LVDPmax, mmHg) in groups CF, C, F and control group was 104.81±17.05, 80.97±21.60, 72.07±26.17 and 71.42±17.55 respectively with statistically significant differences in group CF compared with the other groups (P<0.05). Pathological examination and PCR indicated that transplanted cardiomyocytes in group CF survived better than those in the other groups.Conclusion Transplanted neonatal cardiomyocytes plus fibrin sealant can survive in myocardial infarctioned area and improve heart function greatly in rat models.

  12. Autologous platelet gel and fibrin sealant enhance the efficacy of total knee arthroplasty : improved range of motion, decreased length of stay and a reduced incidence of arthrofibrosis

    NARCIS (Netherlands)

    Everts, Peter A. M.; Devilee, Roger J. J.; Oosterbos, Cornelis J. M.; Mahoney, Christine Brown; Schattenkerk, Maarten Eeftinck; Knape, Johannes T. A.; van Zundert, Andre

    2007-01-01

    In this study we describe the potential role of autologous platelet gel and fibrin sealant in unilateral total knee arthroplasty to improve the postoperative range of motion and to reduce the incidence of arthrofibrosis. Total knee arthroplasty is often associated with a considerable amount of post-

  13. Reduction of the closure time of postoperative enterocutaneous fistulas with fibrin sealant

    Institute of Scientific and Technical Information of China (English)

    Jorge; Avalos-González; Eliseo; Portilla-deBuen; Caridad; Aurea; Leal-Cortés; Abel; Orozco-Mosqueda; María; del; Carmen; Estrada-Aguilar; Gabriela; Abigail; Velázquez-Ramírez; Gabriela; Ambriz-González; Clotilde; Fuentes-Orozco; Aldo; Emmerson; Guzmán-Gurrola; Alejandro; González-Ojeda

    2010-01-01

    AIM: To assess whether the use of fibrin sealantshortens the closure time of postoperative enterocutaneous fistulas (ECFs). METHODS: The prospective case-control study included 70 patients with postoperative ECFs with an output of 2 cm and without any local complication. They were divided into study (n = 23) and control groups (n = 47). Esophageal, gastric and colocutaneous fistulas were monitored under endoscopic visualization, which also allowed fibrin glue application d...

  14. Fibrin-genipin annulus fibrosus sealant as a delivery system for anti-TNFα drug

    Science.gov (United States)

    Likhitpanichkul, Morakot; Kim, Yesul; Torre, Olivia M; See, Eugene; Kazezian, Zepur; Pandit, Abhay; Hecht, Andrew C

    2015-01-01

    Background context Intervertebral discs (IVD) are attractive targets for local drug delivery because they are avascular structures with limited transport. Painful IVDs are in a chronic inflammatory state. While anti-inflammatories show poor performance in clinical trials their efficacy treating IVD cells suggests that sustained, local drug delivery directly to painful IVDs may be beneficial. Purpose To determine if genipin crosslinked fibrin (FibGen) with collagen type I hollow spheres (CHS) can serve as a drug delivery carrier for the anti-TNFα drug, infliximab. Infliximab was chosen as a model drug because of the known role of TNFα in increasing downstream production of several pro-inflammatory cytokines and pain mediators. FibGen was used as drug carrier because it is adhesive injectable, slowly degrading hydrogel with potential to seal annulus fibrosus (AF) defects. CHS allow simple and non-damaging drug loading and could act as a drug reservoir to improve sustained delivery. Study Design/Setting Biomaterials and human AF cell culture study to determine drug release kinetics and efficacy. Methods Infliximab was delivered at low and high concentrations using FibGen with and without CHS. Gels were analyzed for structure, drug release kinetics, and efficacy treating human AF cells following release. This work was funded by grants from the NIAMS/NIH (R01 AR057397), AO Foundation, and from the Icahn School of Medicine at Mount Sinai. Results Fibrin showed rapid infliximab drug release but degraded quickly. CHS alone showed a sustained release profile but the small spheres may not remain in a degenerated IVD with fissures. FibGen showed steady and low levels of infliximab release that was increased when loaded with higher drug concentrations. Infliximab was bound in CHS when delivered within FibGen and was only released following enzymatic degradation. The infliximab released over 20 days retained its bioactivity as confirmed by the sustained reduction of IL-1

  15. Does Fibrin Glue Sealant Used During Pancreaticoduodenectomy Reduce Post-Operative Complication? Audit of 100 Consecutive Patients Undergoing Whipple's Procedure by a Single Surgeon

    Directory of Open Access Journals (Sweden)

    Shafiq Rehman

    2016-03-01

    Full Text Available Introduction This study evaluated the effect of fibrin glue applied as a sandwich film between a two layer pancreatico-jejunostomy anastomosis following pancreaticoduodenectomy. Primary end-points were post-operative pancreatic fistulae, overall complication rates and post-operative length of stay. Methods Pancreaticoduodenectomy was performed by fashioning a two layer pancreatico-jejunostomy with or without a glue sealant which when applied, formed a thin film external to the ductal anastomosis but internal to the seromuscularpancreatic parenchymal layer. Results Following audit of 100 consecutive patients undergoing pancreaticoduodenectomy, patients were separated into two groups; those with glue augmented anastomosis [Glue (G N=50] or those without [No Glue (NG, N=50]. Each group was matched with regard to age [median, G=68 years vs. NG=66 years, (P=0.19] and sex (P=0.84. There were no statistically significant differences between the two groups with respect to overall POPF [G N=7(14% vs. NG N=11(22%, (P=0.42], significant complications (Clavien Grade 3 or more, [G N=4(8% vs. NG N=2(4% (P=0.40], or post-operative length of stay [G 13 days vs. NG 14 days, (P=0.90]. In those patients with the highest fistulae risk score, there were significantly more post-operative pancreatic fistulae in the no glue cohort. There was no mortality in either group. Conclusion This study shows that application of sealant glue significantly reduces post-operative pancreatic fistulae in high-risk patients, but does not reduce overall complications or hospital stay following pancreaticoduodenectomy.

  16. Laparoscopic intraperitoneal mesh fixation with fibrin sealant (Tisseel®) vs. titanium tacks: a randomised controlled experimental study in pigs

    DEFF Research Database (Denmark)

    Eriksen, J.R.; Bech, Jakob Ilsted; Linnemann, D.

    2008-01-01

    chronic) pain after LVHR. Therefore, non-invasive and patient-friendly mesh fixation methods must be considered. The present study was designed to investigate the technical applicability, safety and effect of Tisseel (R) for intraperitoneal mesh fixation. Methods Nine 40-kg Danish Landrace female pigs had...... two pieces of MotifMESH (R) and two pieces of Proceed (R) mesh fixed in the intraperitoneal position by a laparoscopic technique. The two pieces of the same mesh were fixed with fibrin glue (Tisseel) and titanium tacks, respectively. All pigs were euthanised on the 30th postoperative day and the mesh...... feasible in a pig model. There is still no evidence that fibrin-sealing alone is appropriate for intraperitoneal mesh fixation in hernia repair, but the technique might become an alternative or supplement to mechanical mesh fixation. Until then, further experimental research in animal hernia models...

  17. Laparoscopic intraperitoneal mesh fixation with fibrin sealant (Tisseel((R))) vs. titanium tacks: a randomised controlled experimental study in pigs

    DEFF Research Database (Denmark)

    Eriksen, J.R.; Bech, J.I.; Linnemann, D.

    2008-01-01

    chronic) pain after LVHR. Therefore, non-invasive and patient-friendly mesh fixation methods must be considered. The present study was designed to investigate the technical applicability, safety and effect of Tisseel((R)) for intraperitoneal mesh fixation. METHODS: Nine 40-kg Danish Landrace female pigs...... had two pieces of MotifMESH((R)) and two pieces of Proceed((R)) mesh fixed in the intraperitoneal position by a laparoscopic technique. The two pieces of the same mesh were fixed with fibrin glue (Tisseel) and titanium tacks, respectively. All pigs were euthanised on the 30th postoperative day...... with Tisseel is safe and technically feasible in a pig model. There is still no evidence that fibrin-sealing alone is appropriate for intraperitoneal mesh fixation in hernia repair, but the technique might become an alternative or supplement to mechanical mesh fixation. Until then, further experimental...

  18. Successful closure of a bronchopleural fistula by intrapleural administration of fibrin sealant: A case report with review of Literature

    Directory of Open Access Journals (Sweden)

    Pranabh Shrestha

    2014-01-01

    Full Text Available Context: There are no established guidelines for the proper treatment of patients with bronchopleural fistulas (BPFs. Apart from attempts to close the fistula, emphasis of treatment and management is placed on preventive measures, early administration of antibiotics, drainage of the empyema and aggressive nutritional and rehabilitative support. Case Report: A 53-year-old male presented with nausea, vomiting, and dry cough with eventual respiratory failure. He was found to have an empyema of the left hemithorax which was managed with thoracostomy drainage and antibiotics. However, he had persistent air leak through the chest tube due to a BPF. Bronchoscopy failed to localize the involved segment. Application of fibrin glue through the chest tube succeeded in completely sealing the leak. Conclusion: To our knowledge, this is the first case report in which fibrin glue was successfully used intrapleurally to close a BPF related to an empyema.

  19. Collagen fleece-bound fibrin sealant is not associated with an increased risk of thromboembolic events or major bleeding after its use for haemostasis in surgery: a prospective multicentre surveillance study

    Directory of Open Access Journals (Sweden)

    Günther Klaus

    2009-06-01

    Full Text Available Abstract Background Topical haemostatic agents are used to help achieve haemostasis during surgery when standard surgical techniques are insufficient. The objective of this study was to confirm the safety profile of an equine collagen patch coated with human fibrinogen and human thrombin with particular focus on the occurrence of thromboembolic events (TEEs, major bleeding and immunological events. Methods This was a non-interventional, multicentre, prospective, surveillance study in which a collagen fleece-bound fibrin sealant was prescribed in accordance with its marketing authorisation. The decision to use the sealant was based solely on current surgical practice. All patients that received the sealant and provided informed consent were included. TEEs (any coagula-based occlusion in a vessel or the heart identified by symptomatic clinical signs and/or verified by paraclinical examination, major bleeding (any bleeding that required intervention, and immunological events (hypersensitivity including anaphylaxis that occurred during surgery, post-operative hospital stay or 6 months of follow-up were reported as adverse events. The primary endpoint was the proportion of patients experiencing a confirmed TEE. Results A total of 3098 patients were recruited at 227 centres in 12 European countries. The most frequent types of surgery were hepatic (33%, gastrointestinal (16% and urological (14% and the main indication for surgery was for primary (35% or secondary (20% malignancy. Forty-six patients (1.5%, 95% CI 1.1–2.0% had at least one TEE during the study. The most commonly reported TEEs were pulmonary embolism or post-procedural pulmonary embolism (n = 18 and deep vein thrombosis (n = 9. There were 64 major bleedings in 62 patients and 9 immunological events in 8 patients. Conclusion Collagen fleece-bound fibrin sealant does not appear to be associated with an increased risk of TEEs, major bleeding or immunological events in patients undergoing

  20. FIBRIN GLUE DAN APLIKASINYA

    Directory of Open Access Journals (Sweden)

    Agi Harliani S

    2015-08-01

    Full Text Available Fibrin Tissue Adhesive (FTA, Fibrin Sealant (FS or Fibrin Glue (FG are names given to a group of product that lead to the formation of fibrin clot at the site of application. Fibrin Glue represents a new revolution for local haemostatic, which produced by based on the understanding about blood coagulation process. The mechanism of FG mimics the last stage of blood coagulation process. Haemophilia, is a congenital inherited bleeding disorder, characterized by repeated bleeding episodes. The basic pathology is deficiency of factor VIII (hemophilia A or factor IX (hemophilia B. At bleeding episodes, hemophilia patients need replacement therapy. Hemophilia patients need transfusion of cryoprecipitate, Fresh Frozen Plasma (FFP or factor concentrate as replacement therapy. Oral surgery, dental extraction, circumcision, and orthopedic operations are the most important indications for fibrin glue in hemophilia care. As haemostatic local, FG minimizes bleeding, reducing the need of transfusion or factor concentrate, reducing the complication of transfusion, hospitalization and cost.

  1. Collagen fleece-bound fibrin sealant is not associated with an increased risk of thromboembolic events or major bleeding after its use for haemostasis in surgery: a prospective multicentre surveillance study

    DEFF Research Database (Denmark)

    Birth, Mathias; Figueras, Joan; Bernardini, Stéphane

    2009-01-01

    ), major bleeding (any bleeding that required intervention), and immunological events (hypersensitivity including anaphylaxis) that occurred during surgery, post-operative hospital stay or 6 months of follow-up were reported as adverse events. The primary endpoint was the proportion of patients....... CONCLUSION: Collagen fleece-bound fibrin sealant does not appear to be associated with an increased risk of TEEs, major bleeding or immunological events in patients undergoing surgery. TRIAL REGISTRATION: Clinicaltrials.gov number: NCT00285623.......ABSTRACT: BACKGROUND: Topical haemostatic agents are used to help achieve haemostasis during surgery when standard surgical techniques are insufficient. The objective of this study was to confirm the safety profile of an equine collagen patch coated with human fibrinogen and human thrombin...

  2. 医用生物蛋白胶对注射型骨形态发生蛋白成骨活性的影响%Influence of fibrin sealant on osteoinductive ability of inject-type bone morphogenetic protein

    Institute of Scientific and Technical Information of China (English)

    王登虎; 刘建; 李丹; 胡蕴玉; 袁志

    2002-01-01

    Objective To observe influence of fibrin sealant(FS) on osteoinductive ability of inject type BMP.Method The inject type BMP power was dissolved in the main glue part or thrombin part of FS, then mixed with the main glue part or thrombin part of FS into gel, observe coagulating time, then implant composite into the thigh muscle pouch of mice to evaluate their capacity to induce new bone formation, and compared to the single BMP implant group.Result There was no difference in the coagulating time between two mixing method, the osteoinductive ability of implants BMP dissolved in the main glue part or thrombin part of FS group was higher than that of simply BMP implant group.Conclusion FS was perfect carrier to inject type BMP.

  3. [Experimental study on application recombinant human bone morphogenetic protein 2(rhBMP-2)/poly-lactide-co-glycolic acid (PLGA)/fibrin sealant(FS) on repair of rabbit radial bone defect].

    Science.gov (United States)

    Fan, Zhongkai; Cao, Yang; Zhang, Zhe; Zhang, Mingchao; Lu, Wei; Tang, Lei; Yao, Qi; Lu, Gang

    2012-10-01

    This paper is aimed to investigate the repair of rabbit radial bone defect by the recombinant human bone morphogenetic protein 2/poly-lactideco-glycolic acid microsphere with fibrin sealant (rhBMP-2/PLGA/FS). The radial bone defect models were prepared using New Zealand white rabbits, which were randomly divided into 3 groups, experiment group which were injected with eMP-2/PLGA/FS at bone defect location, control group which were injected with FS at bone defect location, and blank control group without treatment. The ability of repairing bone defect was evaluated with X-ray radiograph. Bone mineral density in the defect regions was analysed using the level of ossification. The osteogenetic ability of repairing bone defect, the degradation of the material, the morphologic change and the bone formation were assessed by HE staining and Masson staining. The result showed that rhBMP-2/PLGA/FS had overwhelming superiority in the osteogenetic ability and quality of bone defect over the control group, and it could promote the repair of bone defect and could especially repair the radial bone defect of rabbit well. It may be a promising and efficient synthetic bone graft.

  4. Dental sealants

    Science.gov (United States)

    ... few quick steps. There is no drilling or scraping of the molars. Your dentist will: Clean the ... Dental sealants. Updated October 19, 2016. ADA.org Web site. www.ada.org/en/member-center/oral- ...

  5. T tube sinus fibrin sealant implantation to assist the treatment of biliary fistula after removal of T tube%经T管窦道生物蛋白胶植入协助治疗拔T管后胆漏

    Institute of Scientific and Technical Information of China (English)

    任贵兵; 毛中鹏; 吴凤云; 张瑞奎; 马熙; 王磊

    2013-01-01

    Objective To study the treatment approach of biliary fistula after removal of T tube, in the process of laparoscopic choledocholithotomy with T tube drainage (LCTD). And, on the basis of clinical routine methods, to discuss the feasibility of fibrin glue to block T tube sinus orifice. Methods The research objects were collected for the LCTD postoperatively, biliary fistula found after the removal of T tube. Thirty-six cases were randomly divided into 2 groups. A control group of 18 cases, when bile leakage found after the removal of the T tube, food and water fasting, gastrointestinal decompression, acid and enzyme inhibition, anti infection treatments were carried out. At the same time, the endoscopic T tube sinus catheter was placed or endoscopic nasobiliary drainage undergone. The study group of 18 cases, when bile leakage found after the removal of the T tube, in addition to the routine methods carried out same as the control group, fibrin glue sealant was implanted through T tube sinus by choledochofiberscopy, to block the sinus laceration or the incomplete healing. Recovery conditions in patients of two groups were compared and statistically analyzed. Results In the control group, signs of peritonitis recovered more slowly, treatment processed for a longer time, and costs higher. Two patients underwent a second operation. In the study group, signs of peritonitis recovery more quickly, with shorter treatment time and lower cost, no second operation needed in all patients. Comparison between the two groups, each index had a significant difference (P <0.05). Conclusion For patients with bile leakage after the removal of the T tube, in addition to the routine methods of food and water fasting, gastrointestinal decompression, acid and enzyme inhibition, anti infection, endoscopic T tube sinus catheter placed or endoscopic nasobiliary drainage undergone, the implantation of fibrin glue sealant can improve the recovery speed, with lower cost, no second operation

  6. 经内镜下注射人纤维蛋白粘合剂治疗7例食管癌术后胸内吻合口漏临床研究%The application of endoscopic injection of fibrin sealant in repair of intrathoracic anastomotic leakage after radical resection of esophageal carcinoma

    Institute of Scientific and Technical Information of China (English)

    陈学瑜; 袁晓琴; 陈中元

    2015-01-01

    目的:探讨经内镜下注射人纤维蛋白粘合剂在食管癌术后胸内吻合口漏患者治疗中的应用效果。方法回顾性分析7例术后发生胸内吻合口漏的胸内吻合的食管癌患者临床资料,比较内镜下注射人纤维蛋白粘合剂治疗前后患者吻合口旁引流量、体温、血C反应蛋白(C-reaction protein,CRP)、血白细胞计数等指标的变化。结果经内镜下注射人纤维蛋白粘合剂治疗后7患者吻合口旁平均引流量、白细胞计数、CRP水平均明显降低,与治疗前比较差异具有统计学意义(P<0.05)。治疗前后患者体温差异无统计学意义(P>0.05)。结论经内镜下注射人纤维蛋白粘合剂封闭吻合口漏口可有效降低漏口周围渗出,减轻患者全身炎症反应,促进患者术后恢复。%Objective To evaluate the clinical value of endoscopic injection of fibrin sealant in patients with intra-thoracic anastomotic leakage after radical resection of esophageal carcinoma. Methods The clinical data of 7 pa-tients suffering intrathoracic anastomotic leakage were analyzed. The drainage volume adjacent to the anastomosis, body temperature, white blood cell (WBC) count and CRP of those patients before and after the treatment were com-pared. Result The drainage volume adjacent to the anastomosis, WBC and CRP of the 7 patients were significantly decreased after endoscopic injection of fibrin sealant therapy, and significant differences were observed in comparison between the pre- and post-treatment values of these measures (P0.05). Conclusion Endoscopic injection of fibrin sealant is effective in reducing anasto-motic exudates, alleviating systemic inflammatory response, and may promote postoperative recovery.

  7. 应用生物蛋白胶与胚胎软骨细胞混合移植修复兔膝关节实验性软骨缺损区%Repair of experimental defects of articular cartilage in rabbits with homografts of fibrin sealant and embryonic chondrocytes

    Institute of Scientific and Technical Information of China (English)

    陆敏安; 杨渊; 肖增明; 李世德

    2005-01-01

    BACKGROUND:Research about the repair of articular cartilage with heterograft chondrocytes is frequently reported, but the method may cause immune rejection. Since the embryonic cells possess lower antigenicity and stronger proliferation capability, it is hoped that they can be used as a novel carrier substitute in tissue engineering research.DESIGN: A randomized grouping observation and comparative experiment.SETTING: Histological Embryonic Laboratory in Guangxi Medical University.MATERIALS: A big white adult New Zealand rabbit pregnant for 4 weeks was adopted; and another 24 big white adult New Zealand rabbits were selected, with no limitationin whether they were female or male and with a body mass of 2 to 2.5 kg.METHODS: This experiment was carried out at the Histological Embryonic Laboratory in Guangxi Medical University between December 2000and June 2002. The models of defects in articular cartilage were made artificially in femur medial malleolus of the mature rabbits. In the experimental group, defects were repaired by the implantation of Fibrin Sealant and embryonic chondrocytes mixture, but for the control group, only Fibrin Sealant was implanted or nothing was done about the defect. The restoration of articular cartilage defect was then observed 4,8 and 12 weeks after the operation, and was scored according to modified Pineda's method. The standard consists of 5 items, I.e., cellular morphology, matrix staining, surfacing smoothness, cartilage thickness and host union. 0 refers to normal and the higher the score is, the more serious the pathological changes are.MAIN OUTCOME MEASURES: ①The general observation of rabbit knee joint; ② Histological observation of rabbit knee joints; ③ Histological semi-quantitative score of articular cartilage; ④ Appraisal of the curative effect of articular cartilage defects.RESULTS: Totally 24 rabbits were enrolled in this experiment and all entered the stage of result analysis. ① The general observation of rabbit

  8. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, R.; Dziegiel, M.H.; Agren, M.S.

    2008-01-01

    Platelet-rich fibrin (PRF) is an autologous fibrin sealant (FS) enriched with a platelet concentrate (> 1,000,000 platelets/microL) produced by the automated Vivostat system and used to enhance wound healing. The effects of PRF were compared with supernatant from thrombin-activated platelet...

  9. Application of Biomedical Fibrin Sealant in 18 Patients with Upper Gastrointestinal Ulcerative Perforation%腹腔镜下医用生物蛋白胶粘堵治疗上消化道溃疡穿孔18例报告

    Institute of Scientific and Technical Information of China (English)

    方子兴; 黄建军; 曹水江; 王扬州; 胡立江; 陈虎成; 蒋清华

    2008-01-01

    目的:探讨腹腔镜下医用生物蛋白胶(biomedical fibrin sealant,BFS)粘堵胃、十二指肠球部溃疡穿孔的应用价值.方法:腹腔镜下手术治疗上消化道溃疡穿孔18例.先将明胶海绵填塞入穿孔处,再在其表面和周围浆膜面喷洒医用生物蛋白胶1.5~2.0 mL.结果:18例均痊愈出院.手术时间30~90 min,平均45 min,术后住院4~8 d,平均5.5 d.结论:腹腔镜下应用医用生物蛋白胶粘堵治疗胃、十二指肠球部溃疡穿孔方法,具有疗效可靠,痛苦小、恢复快、并发症少、住院时间短和操作简便等优点,值得推广使用.

  10. Failure mechanisms of fibrin-based surgical tissue adhesives

    Science.gov (United States)

    Sierra, David Hugh

    A series of studies was performed to investigate the potential impact of heterogeneity in the matrix of multiple-component fibrin-based tissue adhesives upon their mechanical and biomechanical properties both in vivo and in vitro. Investigations into the failure mechanisms by stereological techniques demonstrated that heterogeneity could be measured quantitatively and that the variation in heterogeneity could be altered both by the means of component mixing and delivery and by the formulation of the sealant. Ex vivo tensile adhesive strength was found to be inversely proportional to the amount of heterogeneity. In contrast, in vivo tensile wound-closure strength was found to be relatively unaffected by the degree of heterogeneity, while in vivo parenchymal organ hemostasis in rabbits was found to be affected: greater heterogeneity appeared to correlate with an increase in hemostasis time and amount of sealant necessary to effect hemostasis. Tensile testing of the bulk sealant showed that mechanical parameters were proportional to fibrin concentration and that the physical characteristics of the failure supported a ductile mechanism. Strain hardening as a function of percentage of strain, and strain rate was observed for both concentrations, and syneresis was observed at low strain rates for the lower fibrin concentration. Blister testing demonstrated that burst pressure and failure energy were proportional to fibrin concentration and decreased with increasing flow rate. Higher fibrin concentration demonstrated predominately compact morphology debonds with cohesive failure loci, demonstrating shear or viscous failure in a viscoelastic rubbery adhesive. The lower fibrin concentration sealant exhibited predominately fractal morphology debonds with cohesive failure loci, supporting an elastoviscous material condition. The failure mechanism for these was hypothesized and shown to be flow-induced ductile fracture. Based on these findings, the failure mechanism was

  11. 纤维蛋白粘合剂在心脏手术止血中应用的初步研究%Clinical Application of Fibrin Sealant in Cardiac Surgery

    Institute of Scientific and Technical Information of China (English)

    王哲; 李健; 周光华; 陈安清; 蒋振斌

    2003-01-01

    纤维蛋白粘合剂(fibrin sealant,FS)也称为纤维蛋白胶,其主要成分是已纯化、经病毒灭活处理的人纤维蛋白原和人凝血酶,有时还加入经病毒灭活处理的人XⅢ因子和牛抑肽酶。该制剂在欧洲、加拿大、日本等地应用广泛.尤其在心脏外科领域。本实验观察莱士公司生产的纤维蛋白胶在心脏外科手术中的止血效果及安全性,仅为初步研究。

  12. Microvascular anastomosis using fibrin glue and venous cuff in rat carotid artery.

    Science.gov (United States)

    Sacak, Bulent; Tosun, Ugur; Egemen, Onur; Sakiz, Damlanur; Ugurlu, Kemal

    2015-04-01

    Conventional anastomosis with interrupted sutures can be time-consuming, can cause vessel narrowing, and can lead to thrombosis at the site of repair. The amount of suture material inside the lumen can impair the endothelium of the vessel, triggering thrombosis. In microsurgery, fibrin sealants have the potential beneficial effects of reducing anastomosis time and promoting accurate haemostasis at the anastomotic site. However, there has been a general reluctance to use fibrin glue for microvascular anastomoses because the fibrin polymer is highly thrombogenic and may not provide adequate strength. To overcome these problems, a novel technique was defined for microvascular anastomosis with fibrin glue and a venous cuff. Sixty-four rats in two groups are included in the study. In the experimental group (n = 32), end-to-end arterial anastomosis was performed with two stay sutures, fibrin glue, and a venous cuff. In the control group (n = 32), conventional end-to-end arterial anastomosis was performed. Fibrin glue assisted anastomosis with a venous cuff took less time, caused less bleeding at the anastomotic site, and achieved a patency rate comparable to that provided by the conventional technique. Fibrin sealant assisted microvascular anastomosis with venous cuff is a rapid, easy, and reliable technique compared to the end-to-end arterial anastomosis.

  13. Treatment of gingival recession using free gingival graft with fibrin fibronectin sealing system: A novel approach.

    Science.gov (United States)

    Srinivas, B V V; Rupa, N; Halini Kumari, K V; Rajender, A; Reddy, M Narendra

    2015-08-01

    Periodontal plastic surgery is the branch of periodontology that is focused mainly on the correction or elimination of mucogingival problems associated with lack of attached gingiva, a shallow vestibule and aberrant frenum. Various mucogingival surgical procedures are used to halt the progression of the gingival recession and to correct poor esthetic appearance. Free gingival autograft is one of the most common techniques used for a gingival recession in areas of inadequate attached gingiva in the mandibular anterior region. Fibrin sealants are human plasma derivatives that mimic the final stages of blood coagulation, forming a fibrin clot. Fibrin Sealants enhances the overall outcome of surgical intervention because of their hemostatic, adhesive, and healing properties. These properties of fibrin sealants may reduce operating time, prevent complications, and enhance the overall outcome of many surgical interventions. Hence, this case report aims to investigate the clinical effectiveness of free gingival graft along with the commercially available fibrin-fibronectin sealing system (Tissucol(®)) in the treatment of Miller's class II gingival recession.

  14. Experimental study of coralline hydroxyapatite porous,fibrin sealant and staphylococcus aureus injection compound in bone defect restoration%多孔羟基磷灰石、纤维蛋白和金葡液复合物修复骨缺损的实验研究

    Institute of Scientific and Technical Information of China (English)

    韦正超; 蔡道章; 张峻峰; 金文涛

    2004-01-01

    背景:骨缺损的修复一直是骨科治疗的难点,寻找有效修复骨缺损的骨移植替代材料是目前骨缺损治疗的研究方向.目的:探讨珊瑚多孔羟基磷灰石(coralline hydroxyapatite porous,CHAP)、纤维蛋白(fibrin sealant,FS)及金葡液(staphylococcus aureus injection,SAI)复合物修复骨缺损的作用及其作为人工骨移植替代材料的可行性.设计:随机对照的试验研究.单位:中山大学附属第三医院骨科.材料:实验在中山大学动物实验室和解放军第一军医大学全军生物力学中心完成.羟基磷灰石,纤维蛋白,金葡液.方法:采用新西兰大白兔54只在兔双侧桡骨制备骨缺损模型后分成实验组、对照组及空白组.将CHAP-FS-SAI复合物植入骨缺损处作为实验组,以自体骨植入作为对照组,空白组不植入任何物质.在2,4,8和12周分别进行大体标本观察,组织学,X射线片观察及生物力学测试,比较各组修复骨缺损的能力.主要观察指标:动物一般情况,大体标本,X射线片,组织学,生物力学测定.结果:实验组术后2周见植入物与骨端形成紧密的纤维性连接,镜下可见CHAP周围大量成纤维细胞、软骨细胞及细胞钙化.12周实验组和对照组均见大量成熟的骨细胞及板层骨;实验组见植入物完全骨化,塑形完全,CHAP未完全降解.空白组12周骨缺损区为纤维瘢痕组织填充.镜下主要为大量成纤维细胞.X射线片:2周实验组与对照组有骨痂影.4周骨痂影增多.8周实验组骨缺损消失,CHAP分散在骨痂中;对照组骨折线消失,髓腔开始形成.12周实验组和对照组骨皮质连续,髓腔复通,塑形完全.空白对照组12周骨缺损区无骨性连接.生物力学测试最大扭矩及抗扭刚度在术后4,8,12周复合物组和自体骨组差异无显著性意义(P>0.05).但术后2周,最大扭距实验组为(0.140±0.032)N·m,对照组为(0.105±0.035)N·m,抗扭刚度两组分别为(0.401±0

  15. Use of gelatin-thrombin matrix haemostatic sealant in neurosurgery: Anaesthetic implications and review of literature

    Directory of Open Access Journals (Sweden)

    Puneet Khanna

    2015-01-01

    Full Text Available The topical haemostatic agents have been developed to be used as adjunctive measures to promote haemostasis. These include bone wax, absorbable gel sponges, microfibrillar collagen, oxidised regenerated cellulose, gelatin sponges with thrombin, gelatin-thrombin matrix sealant or fibrin sealants. Gelatin-thrombin matrix sealant is a mixture of a bovine-derived gelatin matrix and human-derived thrombin component that are mixed together at the time of use. This agent has been found to be more effective haemostat than thrombin-soaked gelatine sponges. The possible adverse effects of this can be transmission of diseases from human or bovine sources, allergic reactions, thromboembolism, disseminated intravascular coagulopathy (DIC, perilesional oedema, and compression of neural tissue. Although it is used routinely in the operating room, there is little literature available on the perioperative implications with use of intraoperative gelatin-thrombin matrix sealant. Here, we present clinical report of 20 neurosurgical patients where the sealant was used and literature in view of current evidence has been reviewed.

  16. Effect of alkyl chain length on the interfacial strength of surgical sealants composed of hydrophobically-modified Alaska-pollock-derived gelatins and poly(ethylene)glycol-based four-armed crosslinker.

    Science.gov (United States)

    Mizuta, Ryo; Ito, Temmei; Taguchi, Tetsushi

    2016-10-01

    Surgical sealants are widely used clinically. Fibrin sealant is a commonly used sealant, but is ineffective under wet conditions during surgery. In this study, we developed surgical sealants composed of hydrophobically modified Alaska-pollock-derived gelatins (hm-ApGltns) with different alkyl chain lengths from C3 to C18 and a poly(ethylene)glycol-based 4-armed crosslinker (4S-PEG). The burst strength of the hm-ApGltns-based sealant was evaluated using a fresh porcine blood vessel and was found to increase with increasing alkyl chain length from 167±22 to 299±43mmHg when the substitution ratio of amino groups of ApGltn was around 10mol%. The maximum burst strength was observed when stearoyl-group modified ApGltn (Ste-ApGltn)/4S-PEG-based sealant was used, displaying 3-fold higher burst strength than the original ApGltn (Org-ApGltn)/4S-PEG sealant, and 10-fold higher than the commercial fibrin sealant. Ste-ApGltn/4S-PEG-based sealant was biodegraded in rat subcutaneous tissue within 8 weeks without severe inflammation. By molecular interaction analysis using surface plasmon resonance, the binding constant of Ste-ApGltn to fibronectin was found to be 9-fold higher than that of Org-ApGltn. Therefore, the developed sealant, in particular the Ste-ApGltn/4S-PEG-based sealant, has potential applications in the field of cardiovascular surgery as well as thoracic surgery.

  17. Autografting mesenchymal stem cells with fibrin sealant for the therapy of esophageal anastomotic fistula%自体干细胞-纤维蛋白胶联合移植治疗食管吻合口瘘的效果分析

    Institute of Scientific and Technical Information of China (English)

    薛祥; 李春光; 阎岩; 郎希龙; 徐志云; 陈和忠; 张浩

    2015-01-01

    目的 评价自体骨髓来源的间充质干细胞(MSCs)与纤维蛋白胶(FS)联合移植治疗颈部食管吻合口瘘的效果,为治疗食管吻合口瘘探索新方法.方法 将21只成年健康新西兰大白兔完全随机分组,分为实验组(n=12)和对照组(n=9).抽取实验组动物骨髓,培养纯化MSCs,并通过绿色荧光蛋白(GFP)慢病毒载体(Lenti.GFP)转染MSCs.采用切开吻合置管造瘘法建立颈部食管吻合口瘘的动物模型.1周后,确定食管瘘口形成并测量,实验组使用0.2ml含有2×106GFP+ MSCs的纤维蛋白胶封闭瘘口,而对照组应用同体积、不含MSCs的纤维蛋白胶.移植后5周行磁共振成像检查,移植后7周为实验终点,获取食管瘘口部位的组织标本,分别行组织学及免疫组织化学等检测.结果 本研究成功建立了兔颈部食管吻合口瘘的动物模型,建模瘘口大小差异无统计学意义[(2.30±0.15)mm比(2.20 ±0.17) mm,P<0.05],磁共振成像检查显示,实验组瘘口部位仅表现为炎性机化,而对照组多存在化脓性感染.至实验终点,实验组和对照组死亡率差异无统计学意义(3/12比5/9,P=0.20),但实验组闭合率显著高于对照组(10/12比1/9,P=0.02).对瘘口部位标本的组织学研究提示,与对照组相比,实验组炎症反应较轻、胶原纤维规则增生.免疫荧光检测提示,自体移植的MSCs在瘘口部位定植,且向成肌细胞分化.结论 自体干细胞-纤维蛋白胶联合移植可能通过MSCs的免疫调控效应、细胞外基质重构以及纤维蛋白胶对MSCs的抗迁移效应等机制而提高了颈部食管吻合口瘘的闭合率.%Objective To investigate the efficacy of MSCs autografting with fibrin sealant could for the closure of cervical anastomotic fistula.Methods Twenty-one healthy New Zealand rabbits were involved and randomly assigned to treatment group (n =12) or control group (n =9).After the bone marrow were aspirated,the MSCs were isolated,purified and

  18. Does tranexamic acid stabilised fibrin support the osteogenic differentiation of human periosteum derived cells?

    Directory of Open Access Journals (Sweden)

    J Demol

    2011-03-01

    Full Text Available Fibrin sealants have long been used as carrier for osteogenic cells in bone regeneration. However, it has not been demonstrated whether fibrin’s role is limited to delivering cells to the bone defect or whether fibrin enhances osteogenesis. This study investigated fibrin’s influence on the behaviour of human periosteum-derived cells (hPDCs when cultured in vitro under osteogenic conditions in two-dimensional (fibrin substrate and three-dimensional (fibrin carrier environments. Tranexamic acid (TEA was used to reduce fibrin degradation after investigating its effect on hPDCs in monolayer culture on plastic.TEA did not affect proliferation nor calcium deposition of hPDCs under these conditions. Expression profiles of specific osteogenic markers were also maintained within the presence of TEA, apart from reduced alkaline phosphatase (ALP expression (day 14. Compared to plastic, proliferation was upregulated on 2D fibrin substrates with a 220% higher DNA content by day 21. Gene expression was also altered, with significantly (p<0.05 decreased Runx2 (day 7 and ALP (day 14 expression and increased collagen I expression (day 14 and 21. In contrast to plastic, mineralisation was absent on fibrin substrates. Inside fibrin carriers, hPDCs were uniformly distributed. Moderate cell growth and reduced osteogenic marker expression was observed inside fibrin carriers. After 2 weeks, increased cell death was present in the carrier’s centre. In conclusion, fibrin negatively influences osteogenic differentiation, compared to culture plastic, but enhanced proliferation (at least in 2D cultures for hPDCs cultured in osteogenic conditions. TEA maintained the integrity of fibrin-based constructs, with minor effects on the osteogenic differentiation of hPDCs.

  19. [Healing of tubal anastomoses--microsurgery vs. fibrin gluing: morphologic aspects].

    Science.gov (United States)

    Gauwerky, J F; Klose, R P; Forssmann, W G

    1994-01-01

    The healing of anastomoses either by microsurgical suture technique or by fibrin sealant technique has been examined in an experimental morphological study. With view to morphological criteria the healing of tubal anastomoses after one month is completed. Afterwards only little areas of regeneration could be found in the region of the anastomosis. These statements are valid for both types of anastomoses. In single cases a more progressive regeneration of the mucosa could be demonstrated. Using fibrin glue a more pronounced fibrosis could not be seen.

  20. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair.

    Science.gov (United States)

    Schek, R M; Michalek, A J; Iatridis, J C

    2011-04-18

    Treatment of damaged intervertebral discs is a significant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fibrosus. An annular repair material should meet three specifications: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fibrin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fibrin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fibrin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fibrin alone. Finally, lap tests were performed to evaluate adhesion between fibrin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fibrin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fibrin gels show promise as a gap-filling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fibrosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

  1. Delayed cerebrospinal fluid leak after watertight dural closure with a polyethylene glycol hydrogel dural sealant in posterior fossa surgery: case report.

    Science.gov (United States)

    Jito, Junya; Nitta, Naoki; Nozaki, Kazuhiko

    2014-01-01

    A polyethylene glycol (PEG) hydrogel sealant recently has been approved as an adjunct to sutured dural closure in Japan. We treated consecutive six patients with PEG hydrogel sealant in posterior fossa operation. Three of six cases suffered delayed cerebrospinal fluid (CSF) leak after watertight dural closure with the PEG hydrogel sealant, although there was no leak case which was treated with fibrin glue, before 2 years until the adoption of the new material. These patients underwent posterior fossa craniotomy and discharged without remarkable CSF leak. The pseudomeningocele under the occipital wound caused the CSF leak occurr from 5th to 7th week postoperatively. All CSF leak cases needed surgical repair. At the repair, the PEG hydrogel was liquefied and almost absorbed. A fistula on the closure line and a dead space after the absorption of the PEG hydrogel was observed. When the absorbable PEG hydrogel sealant plugs in small gaps of sutured dura, its properties to prevent adhesion might suppress healing process of dural closure, so that CSF could leak through the gaps and collect as a pseudomeningocele in the dead space after absorption of the PEG hydrogel. In posterior fossa surgery a PEG hydrogel sealant should be applied when dural edges are closed tightly without any gaps.

  2. Dental Sealants Prevent Cavities PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2016-10-18

    This 60 second public service announcement is based on the October 2016 CDC Vital Signs report. Dental sealants, applied soon after a child's permanent molars come in, can protect against cavities for up to nine years. Applying sealants in schools for low-income children could save millions in dental treatment costs.  Created: 10/18/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/18/2016.

  3. Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

    Directory of Open Access Journals (Sweden)

    RM Schek

    2011-04-01

    Full Text Available Treatment of damaged intervertebral discs is a significant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fibrosus. An annular repair material should meet three specifications: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fibrin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fibrin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fibrin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fibrin alone. Finally, lap tests were performed to evaluate adhesion between fibrin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fibrin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fibrin gels show promise as a gap-filling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fibrosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

  4. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin

    DEFF Research Database (Denmark)

    Lundquist, Rasmus; Dziegiel, Morten H; Agren, Magnus S

    2008-01-01

    Platelet-rich fibrin (PRF) is an autologous fibrin sealant (FS) enriched with a platelet concentrate (> 1,000,000 platelets/microL) produced by the automated Vivostat system and used to enhance wound healing. The effects of PRF were compared with supernatant from thrombin-activated platelet...... concentrate, recombinant human platelet-derived growth factor (rhPDGF) isoforms, and a homologous FS in cultured normal human dermal fibroblasts. Also, the release of selected endogenous growth factors from PRF and their stability against proteolytic degradation were studied. The proliferative effect of PRF...... exceeded that of FS and rhPDGF-BB, although it was lower than thrombin-activated platelet concentrate possibly due to sustained growth factor release from platelets in PRF. Anti-PDGF antibody blocked the mitogenic effect of rhPDGF-BB but not that of PRF in growth-arrested fibroblasts. PRF promoted...

  5. Análise das aderências resultantes da fixação de telas cirúrgicas com selantes de fibrina e sutura: modelo experimental intraperitoneal Analysis of adhesions resulted from mesh fixation with fibrin sealant and suture: experimental intraperitoneal model

    Directory of Open Access Journals (Sweden)

    Juliano Hermes Maeso Montes

    2012-12-01

    Full Text Available OBJETIVO: Comparar fixação cirúrgica de telas de polipropileno (PP e telas de polipropileno revestido (PCD, usando fio de sutura de polipropileno e cola biológica, quanto à formação de aderências intraperitoneais. MÉTODOS: Amostra de 46 ratas Wistar, randomizadas em seis grupos: dois grupos-controle, com cinco ratas cada, que foram submetidos um à incisão medial (IM e o outro à uma incisão em forma de U (IU; nenhum desses grupos recebeu tela. Dois grupos com tela de PP, um com dez ratas, fixada com sutura (PPF, e o outro, com seis ratas, fixada com cola biológica (PPC. E Dois grupos com tela de PCD, no primeiro, com dez animais, a tela foi fixada com sutura (PCDF, e no segundo, com dez animais, com cola biológica (PCDC. RESULTADOS: Após o prazo de 21 dias, os grupos-controle não apresentaram aderências significantes. O grupo PPC apresentou menor grau de aderência do que o grupo PPF (p=0,01. Não houve diferença entre as fixações nos grupos com PCD. CONCLUSÃO: A comparação da fixação apresentou diferença estatística significativa apenas à tela de PP, com menor grau de aderência utilizando a cola. As aderências se localizaram predominantemente nas extremidades das telas estudadas.OBJECTIVE: To compare surgical fixation of polypropylene mesh (PP and coated polypropylene mesh (PCD using polypropylene suture and fibrin glue, as for the formation of intraperitoneal adhesions. METHODS: A sample of 46 female Wistar rats were randomized into six groups: two control groups, with five rats each, were subjected to one medial incision (MI and the other to a U-shaped incision (UI, none of these groups received the mesh. Two groups of PP mesh, with ten rats, fixed with suture (PPF, the other with six rats, fixed with biological glue (PPC. And two groups of PCD mesh, at first, with ten animals, the mesh was fixed with sutures (PCDFs and the second with ten animals with biological glue (PCDC. RESULTS: After 21 days, the control

  6. Marginal Microleakage of Conventional Fissure Sealants and Self-Adhering Flowable Composite as Fissure Sealant in Permanent Teeth

    OpenAIRE

    Sara Rahimian-Imam; Nahid Ramazani; Mohammad Reza Fayazi

    2015-01-01

    Objectives: Application of sealants is a safe and effective way to prevent occlusal caries in the posterior teeth. A successful sealant therapy depends on good isolation. Decreased steps of adhesive application may enable proper isolation and use of self-adhering flowable composites for sealant therapy. This study sought to compare the marginal microleakage of fissure sealants and self-adhering flowable composites in permanent teeth.Materials and Methods: This in vitro, experimental study was...

  7. Fibrin clot formation and lysis: basic mechanisms

    DEFF Research Database (Denmark)

    Sidelmann, JJ; Gram, J; Jespersen, J;

    2000-01-01

    consequently is an important substrate in the physiology of hemostasis. This review describes the components and processes involved in fibrin formation and fibrin degradation. Particular emphasis is put on the reactions involved in the conversion of fibrinogen to fibrin, the polymerization of fibrin molecules...... induced by coagulation factor XIII (FXIII), and the degradation of fibrinogen and fibrin mediated by plasmin and elastase. Furthermore, factors influencing fibrin structure and fibrin breakdown are addressed; in particular polymorphisms in the genes coding for fibrinogen and FXIII, but also the physical...

  8. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation.

    Science.gov (United States)

    Roy, Sashwati; Driggs, Jason; Elgharably, Haytham; Biswas, Sabyasachi; Findley, Muna; Khanna, Savita; Gnyawali, Urmila; Bergdall, Valerie K; Sen, Chandan K

    2011-11-01

    The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet-rich fibrin matrix (PRFM). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet-embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal-regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.

  9. Application of Glass Sealant for SOFC

    Institute of Scientific and Technical Information of China (English)

    Piao Jinhua; Sun Kening; Zhang Naiqing; Zhou Derui

    2004-01-01

    Glass and glass-ceramic materials were investigated as SOFC seals at 800 ~ 850 ℃. The material was based on the glass and glass-ceramic in the BaO-CaO-Al2O3-SiO2-La2O3-B2O3 system. The sealant has a minimum thermal expansion mismatch with yttria-stabilized zirconia (YSZ)electrolyte and Ni/gYSZ for the anode. The sealant has a superior stability during the process of operation in SOFC and can withstand thermal shock during the process of thermal cycling. The results show that the BaO-CaO-Al2O3-SiO2-La2O3-B2O3 system sealant can be used as sealing materials for SOFC.

  10. Sealants for preventing dental decay in the permanent teeth

    DEFF Research Database (Denmark)

    Ahovuo-Saloranta, Anneli; Forss, Helena; Walsh, Tanya;

    2013-01-01

    or the baseline caries prevalence.- Resin-based sealant compared with no sealant: Compared to control without sealant, second or third or fourth generation resin-based sealants prevented caries in first permanent molars in children aged 5 to 10 years (at 2 years of follow-up odds ratio (OR) 0.12, 95% confidence...... 2012). SELECTION CRITERIA: Randomised or quasi-randomised controlled trials of at least 12 months duration comparing sealants for preventing caries of occlusal or approximal surfaces of premolar or molar teeth with no sealant or different type of sealant in children and adolescents under 20 years...... of age. DATA COLLECTION AND ANALYSIS: Two review authors independently screened search results, extracted data and assessed trial quality. We calculated the odds ratio (OR) for caries or no caries on occlusal surfaces of permanent molar teeth. For trials with a split-mouth design, the Becker...

  11. Biocompatibility, biodegradation, and neovascularization of human single-unit platelet-rich fibrin glue: an in vivo analysis

    Institute of Scientific and Technical Information of China (English)

    Wu Xiuwen; Ren Jianan; Yao Genhong; Zhou Bo; Wang Gefei; Gu Guosheng; Luan Jianfeng

    2014-01-01

    Background The clinical applications of fibrin glue span over several surgical modalities.The aim of this study was to evaluate the biocompatibility and biodegradation of different formulations of platelet-rich fibrin glue in vivo and examine its effects on the neovascularization of wound sites.Methods Human-derived single-unit fibrin glue was prepared.Incisions were made on the backs of rats,and these were coated with homemade glues containing different concentrations of aminomethylbenzoic acid (Groups A-F) or commercial adhesives (Group G).A sham control group was included (Group H).The wounds were examined by histological analysis and immunohistochemistry at several time points.Results Successful wound closure was achieved in all groups by day 12.Acute inflammation occurred during the first six days,but gradually disappeared.The longest sealant duration was achieved using the lowest concentration of antifibrinolytic agent in a 1:10 volume ratio with cryoprecipitate.Expression levels of the platelet endothelial cell adhesion molecule-1 were significantly higher in Groups A and C compared to the control groups (Groups G and H) on day 3 (P <0.05).Conclusions Single-unit platelet-rich fibrin glue has excellent biocompatibility and is associated with the upregulation of neovascularization.The addition of aminomethylbenzoic acid could prevent the degradation of fibrin glue.

  12. Organo-selenium-containing dental sealant inhibits bacterial biofilm.

    Science.gov (United States)

    Tran, P; Hamood, A; Mosley, T; Gray, T; Jarvis, C; Webster, D; Amaechi, B; Enos, T; Reid, T

    2013-05-01

    Oral bacteria, including Streptococcus mutans and Streptococcus salivarius, contribute to tooth decay and plaque formation; therefore, it is essential to develop strategies to prevent dental caries and plaque formation. We recently showed that organo-selenium compounds covalently attached to different biomaterials inhibited bacterial biofilms. Our current study investigates the efficacy of an organo-selenium dental sealant (SeLECT-Defense(TM) sealant) in inhibiting S. mutans and S. salivarius biofilm formation in vitro. The organo-selenium was synthesized and covalently attached to dental sealant material via standard polymer chemistry. By colony-forming unit (CFU) assay and confocal microscopy, SeLECT-Defense(TM) sealant was found to completely inhibit the development of S. mutans and S. salivarius biofilms. To assess the durability of the anti-biofilm effect, we soaked the SeLECT-Defense(TM) sealant in PBS for 2 mos at 37°C and found that the biofilm-inhibitory effect was not diminished after soaking. To determine if organo-selenium inhibits bacterial growth under the sealant, we placed SeLECT-Defense sealant over a lawn of S. mutans. In contrast to a control sealant, SeLECT-Defense(TM) sealant completely inhibited the growth of S. mutans. These results suggest that the inhibitory effect of SeLECT-Defense(TM) sealant against S. mutans and S. salivarius biofilms is very effective and durable.

  13. Sealants for solid oxide fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Fergus, Jeffrey W. [Auburn University, Materials Research and Education Center, 275 Wilmore Laboratories, Auburn University, AL 36849 (United States)

    2005-09-09

    One of the major challenges for implementation of solid oxide fuel cells (SOFCs) is the development of suitable sealant materials to separate the air and fuel. Several approaches have been used to achieve the necessary adherence, mechanical integrity and stability, including both rigid seals (no applied load during operation) and compressive seals (load applied to seal during operation). The most common approach is to use rigid glass or glass-ceramic seals, the properties of which can be tailored specifically for use in SOFCs through variation of the glass composition. However, these ceramic materials are inherently brittle, so metallic, metallic-ceramic and ceramic-ceramic composite seals, in both the rigid and compressive configurations, have been developed. The use of multiphase seals allows for improvement in factors, such as wettability, compliance at interfaces and strain relief, to improve the gas-tightness and stability of the seal. In this paper, the different approaches for developing SOFC sealants are reviewed. (author)

  14. The influence of fibrin sealant on the healing colonic anastomosis : an experimental study in rats

    NARCIS (Netherlands)

    A.C. van der Ham

    1993-01-01

    textabstractThe experiments described in this thesis were performed in order to find methods to influence and improve colonic anastomotic wound healing. It is known that inflammation may affect the healing of colonic anastomoses. Eicosanoids are known mediators of the inflammatory process, being a p

  15. The Potential Utility of Fibrin Sealant Dressing in Repair of Vascular Injury in Swine

    Science.gov (United States)

    2007-01-12

    and veterinarian staff. Prophylactic antibiotics (Naxcel, ceftiofur sodium) were given intramus- cularly once a day for 3 days. Postoperative pain was...unexpectedly. Statistical Analysis The Kruskal-Wallis test (nonparametric ANOVA) was used for comparison of pre- and postoperative hematological ...ment was counted as a successful test in the 8-week group. Hemostasis results are summarized in Table 1. Hematological Measurements The hematological

  16. Simple bone cysts in children treated with intracystic fibrin sealant injection

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Simple bone cyst (SBC), also known as a unicameral or solitary cyst, is a benign fluid-filled cavity found primarily at the proximal ends of long bones in children. Treatment is warranted if the pain or a pathologic fracture occurs or if a child is at risk of pathologic fractures. The spectrum of treatments proposed for SBC management ranges from simple Kirschner wire drill-hole, resection or curettage with or without bone grafting to using a cannulated screw for continuous decompression, steroid injection and the latest experience of the use of percutaneous autologous marrow grafting.1-4 However, clinical practice of these treatments is limited because of their unsatisfactory effect and possible compli- cations.

  17. Viscous Glass Sealants for SOFC Applications

    Energy Technology Data Exchange (ETDEWEB)

    Scott Misture

    2012-09-30

    Two series of silicate glasses that contain gallium as the primary critical component have been identified and optimized for viscous sealing of solid oxide fuel cells operating from 650 to 850°C. Both series of glass sealants crystallize partially upon heat treatment and yield multiphase microstructures that allow viscous flow at temperatures as low as 650°C. A fully amorphous sealant was also developed by isolating, synthesizing and testing a silicate glass of the same composition as the remnant glassy phase in one of the two glass series. Of ~40 glasses tested for longer than 500 hours, a set of 5 glasses has been further tested for up to 1000h in air, wet hydrogen, and against both yttria-stabilized zirconia and aluminized stainless steel. In some cases the testing times reached 2000h. The reactivity testing has provided new insight into the effects of Y, Zr, and Al on bulk and surface crystallization in boro-gallio-silicate glasses, and demonstrated that at least 5 of the newly-developed glasses are viable viscous sealants.

  18. Marginal Microleakage of Conventional Fissure Sealants and Self-Adhering Flowable Composite as Fissure Sealant in Permanent Teeth

    Directory of Open Access Journals (Sweden)

    Sara Rahimian-Imam

    2015-11-01

    Full Text Available Objectives: Application of sealants is a safe and effective way to prevent occlusal caries in the posterior teeth. A successful sealant therapy depends on good isolation. Decreased steps of adhesive application may enable proper isolation and use of self-adhering flowable composites for sealant therapy. This study sought to compare the marginal microleakage of fissure sealants and self-adhering flowable composites in permanent teeth.Materials and Methods: This in vitro, experimental study was conducted on 60 extracted human premolar teeth. The teeth were divided randomly into two groups of 30. In the first group, fissure sealant (Clinpro, 3M ESPE, USA was placed on the teeth. In the second group, self-adhering flowable composite (Vertise Flow, Kerr, USA was applied as the sealant. Then, both groups were immersed in 0.5% fuchsin dye solution for 24 hours. Sectioned samples were observed with a stereomicroscope for the extent of dye penetration. Data were analyzed using SPSS 21 and the Mann-Whitney test (P<0.05.Results: Microleakage in the fissure sealant group was significantly higher than that in the self-adhering flowable composite group (P<0.001.Conclusion: Microleakage was less using self-adhering flowable composite compared to conventional fissure sealant; therefore, self-adhering flowable composite can be used as a suitable fissure sealant in permanent teeth.

  19. Fibrinogen and thrombin concentrations are critical for fibrin glue adherence in rat high-risk colon anastomoses

    Directory of Open Access Journals (Sweden)

    Eliseo Portilla-de Buen

    2014-04-01

    Full Text Available OBJECTIVE: Fibrin glues have not been consistently successful in preventing the dehiscence of high-risk colonic anastomoses. Fibrinogen and thrombin concentrations in glues determine their ability to function as sealants, healers, and/or adhesives. The objective of the current study was to compare the effects of different concentrations of fibrinogen and thrombin on bursting pressure, leaks, dehiscence, and morphology of high-risk ischemic colonic anastomoses using fibrin glue in rats. METHODS: Colonic anastomoses in adult female Sprague-Dawley rats (weight, 250-350 g treated with fibrin glue containing different concentrations of fibrinogen and thrombin were evaluated at post-operative day 5. The interventions were low-risk (normal or high-risk (ischemic end-to-end colonic anastomoses using polypropylene sutures and topical application of fibrinogen at high (120 mg/mL or low (40 mg/mL concentrations and thrombin at high (1000 IU/mL or low (500 IU/mL concentrations. RESULTS: Ischemia alone, anastomosis alone, or both together reduced the bursting pressure. Glues containing a low fibrinogen concentration improved this parameter in all cases. High thrombin in combination with low fibrinogen also improved adherence exclusively in low-risk anastomoses. No differences were detected with respect to macroscopic parameters, histopathology, or hydroxyproline content at 5 days post-anastomosis. CONCLUSIONS: Fibrin glue with a low fibrinogen content normalizes the bursting pressure of high-risk ischemic left-colon anastomoses in rats at day 5 after surgery.

  20. Sealants for preventing dental decay in the permanent teeth

    DEFF Research Database (Denmark)

    Ahovuo-Saloranta, Anneli; Forss, Helena; Walsh, Tanya

    2013-01-01

    in preventing caries in children and adolescents when compared to no sealants. Their effectiveness may be related to the caries prevalence in the population. OBJECTIVES: To compare the effects of different types of fissure sealants in preventing caries in permanent teeth in children and adolescents. SEARCH...... 2012). SELECTION CRITERIA: Randomised or quasi-randomised controlled trials of at least 12 months duration comparing sealants for preventing caries of occlusal or approximal surfaces of premolar or molar teeth with no sealant or different type of sealant in children and adolescents under 20 years...... of age. DATA COLLECTION AND ANALYSIS: Two review authors independently screened search results, extracted data and assessed trial quality. We calculated the odds ratio (OR) for caries or no caries on occlusal surfaces of permanent molar teeth. For trials with a split-mouth design, the Becker...

  1. Use of autologous fibrin glue in dermatologic surgery: application of skin graft and second intention healing

    Directory of Open Access Journals (Sweden)

    Aparecida Machado de Moraes

    Full Text Available OBJECTIVE: To evaluate the efficiency of biological sealant, an autologous fibrin glue, in dermatological surgery. DESIGN: Randomized clinical trial. SETTING: The Dermatology Service of Hospital das Clínicas, Universidade de Campinas (UNICAMP, referral center. PATIENTS: 14 patients with malign epithelial cutaneoos tumors participated in the evaluation, each having two tumors, generally facial and symmetrical, in order to perform a comparative evaluation on the same individual. PROCEDURES: The glue was prepared beforehand with a sample of autologous blood. Surgical extirpation of the tumor was followed by grafts or second intention healing. OUTCOMES: The efficiency of the sealant was then evaluated in relation to hemostasis, adhesion, surgical time and evolution of the granulation tissue, clinically and histologically. RESULTS: Immediate hemostasis and graft adhesion, with a significant reduction of surgical time, and in the open wounds there was immediate hemostasis and a clinical increase in granulation tissue, but with no histological differences among the groups on the 7th day. CONCLUSION: It is an adjuvant resource in skin cancer surgery.

  2. Do light cured ART conventional high-viscosity glass-ionomer sealants perform better than resin-composite sealants: a 4-year randomized clinical trial

    NARCIS (Netherlands)

    Zhang, W.; Chen, X.; Fan, M.W.; Mulder, J.; Huysmans, M.C.D.N.J.M.; Frencken, J.E.F.M.

    2014-01-01

    OBJECTIVE: The hypotheses tested were: the cumulative survival rates of dentin caries lesion-free pits and fissures of ART conventional high-viscosity glass-ionomer sealants with light-curing (high-intensity LED) and glass-carbomer sealants are higher than those of conventional ART sealants and resi

  3. Fibrin ring granulomas in hepatitis A.

    Science.gov (United States)

    Ruel, M; Sevestre, H; Henry-Biabaud, E; Courouce, A M; Capron, J P; Erlinger, S

    1992-12-01

    Fibrin ring granuloma is characterized by a fibrinous ring surrounding a central fat vacuole. It has been found in the liver and bone marrow of patients with Q fever, and occasionally with visceral leishmaniasis, cytomegalovirus, Epstein-Barr virus, Staphylococcus epidermidis infections, Hodgkin's lymphoma, and hypersensitivity to allopurinol. We describe a case of serologically confirmed viral hepatitis A with this lesion in the liver biopsy. A false positive anti-hepatitis A virus IgM result has been excluded. This is, to our knowledge, the second reported case of type A hepatitis with hepatic fibrin ring granulomas. It confirms that hepatitis A should be included in the differential diagnosis of this lesion.

  4. Marginal leakage of two newer glass-ionomer-based sealant materials assessed using micro-CT.

    NARCIS (Netherlands)

    Chen, X.; Cuijpers, V.M.J.I.; Fan, M.; Frencken, J.E.F.M.

    2010-01-01

    OBJECTIVES: To test newer glass-ionomer-based materials as sealant materials. One glass-ionomer sealant was light-cured to obtain an early setting reaction. The null-hypothesis tested was: there is no difference in marginal leakage of sealants produced with high-viscosity glass-ionomer, with and wit

  5. Flow characteristics and sealing ability of fissure sealants.

    Science.gov (United States)

    Barnes, D M; Kihn, P; von Fraunhofer, J A; Elsabach, A

    2000-01-01

    This study evaluated the relationship between fissure sealant viscosity, leakage prevention and the incidence of void formation of five commercially available pit-and-fissure sealants. Seventy-two intact, caries free human pre-molars and molars were divided into six test groups of 12 teeth each. All teeth were cleaned with a flour of pumice prophylaxis followed by etching for 60 seconds with 37% H3PO4, rinsing for 30 seconds and drying with oil-free air. Five commercial, light-cured fissure sealants and an unfilled version of one sealant were applied following manufacturers' instructions. Teeth were thermal cycled for 5000 cycles from 5-50 degrees C with a one-minute dwell time at each temperature. Silver nitrate staining followed by mesiodistal sectioning was performed. Leakage and void formation were evaluated at X50 optical magnification. Viscosity was assessed by syringing the fissure sealants into short pipettes, allowing free flow for 30 seconds and then light curing for one minute. The length of unfilled capillary was measured with a Vernier gauge. All experimental data was subjected to a one-way ANOVA, and where differences were detected, they were identified by a post hoc Tukey hsd test at a priori alpha = 0.05. Based on the conditions of the study, viscosity and flow characteristics had no effect on sealing ability or void formation.

  6. Structural hierarchy governs fibrin gel mechanics.

    Science.gov (United States)

    Piechocka, Izabela K; Bacabac, Rommel G; Potters, Max; Mackintosh, Fred C; Koenderink, Gijsje H

    2010-05-19

    Fibrin gels are responsible for the mechanical strength of blood clots, which are among the most resilient protein materials in nature. Here we investigate the physical origin of this mechanical behavior by performing rheology measurements on reconstituted fibrin gels. We find that increasing levels of shear strain induce a succession of distinct elastic responses that reflect stretching processes on different length scales. We present a theoretical model that explains these observations in terms of the unique hierarchical architecture of the fibers. The fibers are bundles of semiflexible protofibrils that are loosely connected by flexible linker chains. This architecture makes the fibers 100-fold more flexible to bending than anticipated based on their large diameter. Moreover, in contrast with other biopolymers, fibrin fibers intrinsically stiffen when stretched. The resulting hierarchy of elastic regimes explains the incredible resilience of fibrin clots against large deformations.

  7. Migration of PCBs from Sealants to Adjacent Material

    DEFF Research Database (Denmark)

    Andersen, Helle Vibeke; Gunnarsen, Lars Bo; Kampmann, Kristoffer

    2015-01-01

    PCB in sealants can migrate into porous surfaces like concrete and brick and contaminate these materials. The study focuses on an analysis of concentration profiles in relation to alleviation of PCB contamination. The PCB content was determined in 20 samples of concrete and 6 samples of brick...... adjacent to outdoor PCB-containing sealants. The samples were submitted to the laboratory for normal commercial assessment of PCB content in relation to the handling of waste from renovation works in Denmark. The results showed that the PBC concentration in the sealant not necessarily relate...... to the concentration of the adjacent material. The profiles of PCB concentration into the materials showed some variation, though in general there is a tendency of relative higher concentration near the primary source in concrete and longer migration at low concentrations in brick. The concentration profiles seemed...

  8. Bisphenol A in dental sealants and its estrogen like effect

    Directory of Open Access Journals (Sweden)

    Manu Rathee

    2012-01-01

    Full Text Available Bisphenol A or BPA-based epoxy resins are widely used in the manufacture of commercial products, including dental resins, polycarbonate plastics, and the inner coating of food cans. BPA is a precursor to the resin monomer Bis-GMA. During the manufacturing process of Bis-GMA dental sealants, Bisphenol A (BPA might be present as an impurity or as a degradation product of Bis-DMA through esterases present in saliva. Leaching of these monomers from resins can occur during the initial setting period and in conjunction with fluid sorption and desorption over time and this chemical leach from dental sealants may be bioactive. Researchers found an estrogenic effect with BPA, Bis-DMA, and Bis-GMA because BPA lacks structural specificity as a natural ligand to the estrogen receptor. It generated considerable concern regarding the safety of dental resin materials. This review focuses on the BPA in dental sealants and its estrogen-like effect.

  9. Sealants in dentistry: outcomes of the ORCA Saturday Afternoon Symposium 2007

    DEFF Research Database (Denmark)

    Splieth, C H; Ekstrand, K R; Alkilzy, M

    2009-01-01

    management of lesions in enamel and the outer part of the dentine. Sealants are also an interesting concept for caries management in approximal surfaces. Clinical trials suggest that novel techniques of sealing or infiltrating approximal lesions show promise. However, approximal sealing techniques....... Evidence for effectiveness of sealants in controlling caries in posterior teeth implies that sealants should be an integrated part of management of pit and fissure caries. Still, the indication for occlusal sealants seems to be shifting from primary prevention to a therapeutic decision for caries...... are as complex to apply and time-consuming as approximal fillings. The article proposes guidelines for teaching on the use of sealants....

  10. Glass Composition for the use as a Sealant

    OpenAIRE

    Kiebach, Wolff-Ragnar; Hendriksen, Peter Vang

    2014-01-01

    The invention concerns a glass composition for the use as a sealant, particularly in a solid oxide fuel cell (SOFC) or in a solid oxide electrolyser cell (SOEC). The glass composition comprises 35-70 mol% CaO, 5-45 mol% ZnO, 5-50 mol% B2O3, 1-45 mol% SiO2, and 1 mol% or less of each element of the group, comprising Ba, Na and Sr, based on the total glass composition. Furthermore, the invention relates to an SOFC and an SOEC employing a sealant of said glass composition.

  11. Glass Composition for the use as a Sealant

    DEFF Research Database (Denmark)

    2014-01-01

    The invention concerns a glass composition for the use as a sealant, particularly in a solid oxide fuel cell (SOFC) or in a solid oxide electrolyser cell (SOEC). The glass composition comprises 35-70 mol% CaO, 5-45 mol% ZnO, 5-50 mol% B2O3, 1-45 mol% SiO2, and 1 mol% or less of each element...... of the group, comprising Ba, Na and Sr, based on the total glass composition. Furthermore, the invention relates to an SOFC and an SOEC employing a sealant of said glass composition....

  12. Safety and usability of hemostats, sealants, and adhesives.

    Science.gov (United States)

    Burks, Sandra; Spotnitz, William

    2014-08-01

    Hemostats, sealants, and adhesives are an integral part of surgical patient care. Nurses who have knowledge about these agents can better help ensure safe, efficient surgical patient care. As a caregiver and patient advocate, the perioperative nurse must understand the most current information about these agents and be prepared to facilitate the transfer of this knowledge to all caregivers. Information about these agents, including the contraindications, warnings, and precautions associated with their use as well as their preparation and application, is provided here. Algorithms designed to clarify the best options for using hemostats, sealants, and adhesives are included as well.

  13. Dental sealant knowledge, opinion, values and practice of Spanish dentists

    Directory of Open Access Journals (Sweden)

    San Martin Laura

    2013-02-01

    Full Text Available Abstract Background Multiple guidelines and systematic reviews recommend sealant use to reduce caries risk. Yet, multiple reports also indicate that sealants are significantly underutilized. This study examined the knowledge, opinions, values, and practice (KOVP of dentists concerning sealant use in the southwest region of Andalusia, Spain. This is a prelude to the generation of a regional plan for improving children’s oral health in Andalusia. Methods The survey’s target population was dentists working in western Andalusia, equally distributed in the provinces of Seville, Cadiz, and Huelva (N=2,047. A convenience sample of meeting participants and meeting participant email lists (N=400 were solicited from the annual course on Community and Pediatric Dentistry. This course is required for all public health sector dentists, and is open to all private sector dentists. Information on the dentist’s KOVP of sealants was collected using four-part questionnaire with 31, 5-point Likert-scaled questions. Results The survey population demographics included 190 men (48% and 206 women (52% with an average clinical experience of 10.6 (± 8.4 years and 9.3 (± 7.5 years, respectively. A significant sex difference was observed in the distribution of place of work (urban/suburb (p=0.001, but no sex differences between working sector (public/private. The mean ± SD values for each of the four KOVP sections for pit and fissure sealants were: knowledge = 3.57 ± 0.47; opinion = 2.48 ± 0.47; value = 2.74 ± 0.52; and practice = 3.48 ± 0.50. No sex differences were found in KOVP (all p >0.4. Independent of sex: knowledge statistically differed by years of experience and place of work; opinion statistically differed by years of experience and sector; and practice statistically differed by years of experience and sector. Less experienced dentists tended to have slightly higher scores (~0.25 on a Likert 1–5 scale. Statistically significant correlations were

  14. Thrombin interaction with fibrin polymerization sites.

    Science.gov (United States)

    Hsieh, K

    1997-05-15

    Thrombin is central to hemostasis, and postclotting fibrinolysis and wound healing. During clotting, thrombin transforms plasma fibrinogen into polymerizing fibrin, which selectively adsorbs the enzyme into the clot. This protects thrombin from heparin-antithrombin inactivation, thus preserving the enzyme for postclotting events. To determine how the fibrin N-terminal polymerization sites of A alpha 17-23 (GPRVVER) and B beta 15-25 (GHRPLDKKREE) and their analogs may interact with thrombin, amidolysis vs. plasma- and fibrinogen-clotting assays were used to differentiate blockade of catalytic site vs. other thrombin domains. Amidolysis studies suggest GPRVVER inhibition of thrombin catalytic site through hydrophobic interaction, and GPRVVER inhibited clotting. Neither GPRP nor VVER nor the B beta 15-25 homologs inhibited amidolysis. Contrary to heparin, acyl-DKKREE promoted plasma-clotting, but inhibited fibrinogen-clotting. In addition, acyl-DKKREE reversed the anticoagulant effect of heparin (0.1 U/ml) in plasma. The results suggest fibrin B beta 15-25 interaction with thrombin, possibly by blocking the heparin-binding site. Together with the reported fibrin A alpha 27-50 binding to thrombin, polymerizing fibrin appears to initially bind to thrombin catalytic site and exosite-1 through A alpha 17-50, and to another thrombin site through B beta 15-25. As these fibrin sites are also involved in polymerization, competition of the polymerization process with thrombin-binding could subsequently dislodge thrombin from fibrin alpha-chain. This may re-expose the catalytic site and exosite-1, thus explaining the thrombogenicity of clot-bound thrombin. The implications of these findings in polymerization mechanism and anticoagulant design are discussed.

  15. Evolution of fibrin glue applicators.

    Science.gov (United States)

    Marx, Gerard

    2003-10-01

    Fibrin glue (FG) is used worldwide as a potent surgical tool, which establishes hemostasis in wounds and also bonds tissue. The standard FG applicator is based on a dual-syringe system. This review, based mainly on the patent literature, describes the development of the quasi-standard dual syringe system as well as the rise of other FG applicator designs based on mechanical force (ratchet systems), Bernoulli gas flow, positive gas pressure, or electro-servo devices. The packaging of commercial FG components is reviewed within the context of "loading" the FG applicators and the need to minimize the number of needles required to access the packaged (vials) components. Parameters such as internal clogging, homogeneity of spray, the requirement for gas or vacuum house lines, the number of parts that must be handled, and the time required to assemble the applicator, load it, and have it ready for use are also discussed. A rating system is proposed that permits one to use such parameters to rank the various applicator designs, relative to the dual-syringe system. Hopefully, this review will stimulate the design of better FG applicators and packaging required for elective surgery, emergency treatments, and tissue engineering in the 21st century.

  16. 21 CFR 864.7300 - Fibrin monomer paracoagulation test.

    Science.gov (United States)

    2010-04-01

    ... to detect fibrin monomer in the diagnosis of disseminated intravascular coagulation (nonlocalized clotting within a blood vessel) or in the differential diagnosis between disseminated intravascular coagulation and primary fibrinolysis (dissolution of the fibrin in a blood clot). (b) Classification. Class...

  17. The state-of-the-art of ART sealants

    NARCIS (Netherlands)

    Frencken, J.E.F.M.

    2014-01-01

    Sealing caries-prone pits and fissure systems is an effective caries-preventive measure. There are basically two types of sealant materials: glass-ionomer and resin-based materials. Low- and medium-viscosity glass-ionomers were initially used and showed a low level of retention. With the advent of t

  18. Bone induction by composites of bioresorbable carriers and demineralized bone in rats: a comparative study of fibrin-collagen paste, fibrin sealant, and polyorthoester with gentamicin

    DEFF Research Database (Denmark)

    Pinholt, E M; Solheim, E; Bang, G

    1992-01-01

    Host tissue response and heterotopic osteoinduction by composites of demineralized bone matrix and three different substances used as bioresorbable carriers implanted in the abdominal muscles were evaluated by strontium 85 uptake and histology 4 weeks postoperatively in 60 male Wistar rats. Both...

  19. Characterization of the biological effect of fish fibrin glue in experiments on rats: Immunological and coagulation studies

    Science.gov (United States)

    Laidmäe, Ivo; Salum, Tiit; Sawyer, Evelyn S.; Janmey, Paul A.; Uibo, Raivo

    2011-01-01

    Fibrin glues (FG) of human or bovine origin are widely used for haemostasis and wound healing. In addition FGs are studied in many biomedical areas like cell therapy or tissue engineering. As any mammalian plasma products FG-s pose risk of transmission of bacteria, viruses, or prions and may compromise patient homeostasis. In this study, we examined coagulation parameters and immunological status of rats treated with salmon-derived FG. We evaluated the changes in thrombin time, prothrombin activity, and presence of antibodies on 46 Wistar rats. This study shows that salmon-derived FG, injected intraperitoneally, does not cause coagulation disturbances in the peripheral blood. After a first challenge with salmon-derived FG there were low but detectable amounts of antibodies revealed by ELISA and immunoblot. After a second administration there was substantial elevation of antibodies to FG components and other copurifying plasma proteins. Antibody reactivity to human Factor Va, revealed in three animals, was not associated with FG application. Taken together, blood immunological and coagulation parameters support the suitability of salmon-derived FG in the development of fibrin sealants for medical use. PMID:19484773

  20. Chitosan whiskers from shrimp shells incorporated into dimethacrylate-based dental resin sealant.

    Science.gov (United States)

    Mahapoka, Ekamon; Arirachakaran, Pratanporn; Watthanaphanit, Anyarat; Rujiravanit, Ratana; Poolthong, Suchit

    2012-01-01

    A resin-based sealant containing chitosan whiskers was developed for use as a pit and fissure sealer. Chitosan whiskers were synthesized and then characterized using Fourier transform infrared spectrometry and transmission electron microscopy. The whiskers were next incorporated into dimethacrylate monomer at various ratios by weight and subsequently analyzed for their antimicrobial and physical properties. The dimethacrylate-based sealant containing chitosan whiskers had a greater antimicrobial activity than control sealant and they were comparable with antimicrobial commercial resin sealants. The inclusion of the whiskers did not reduce the curing depth or degree of double bond conversion and the reduction in hardness was minimal. In conclusion, a resin-based sealant containing chitosan whiskers can be considered an effective antimicrobial pit and fissure sealant.

  1. No effect of fibrin sealant on drain output or functional recovery following simultaneous bilateral total knee arthroplasty

    DEFF Research Database (Denmark)

    Skovgaard, Christian; Holm, Bente; Troelsen, Anders;

    2013-01-01

    Background and purpose Blood loss after total knee arthroplasty (TKA) may lead to anemia, blood transfusions, and increased total costs. Also, bleeding into the periarticular tissue may cause swelling and a reduction in quadriceps strength, thus impairing early functional recovery...

  2. Application of fibrin glue on endoscopic liposuction

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhong-cun; YANG Jia-feng; CHEN Qian-qian

    2001-01-01

    @@ OBJECTIVE To deserve the effects of FG (Fibrin Glue)on endoscopic liposuction. Methods:From Oct. 1998 to Aug. 2000 forty cases were undergoing for the liposuction. Atthe aids of tumescent teachnique, endoscopy and FG were used, of which 20 casesoperation on their abdominal walls, 8 cases upper abdomen walls and 12 cases ownerabdominal.

  3. UROKINASE MUTANT WITH BETTER FIBRIN SPECIFICITY

    Institute of Scientific and Technical Information of China (English)

    MaZhong; YuRuirong; HuaZichun; ZhuDexu

    1997-01-01

    Urokinsae (UK),one of the principal activators of plasminogen, has been widely used in the clinical therapy of thrombosis. However ,it lacks fibrin specificity ,and can lead to extensive systemic activation of plasminogen when injected into veins in large doses. On the other hand,

  4. Platelet-rich fibrin: the benefits.

    Science.gov (United States)

    Kumar, Yuvika Raj; Mohanty, Sujata; Verma, Mahesh; Kaur, Raunaq Reet; Bhatia, Priyanka; Kumar, Varun Raj; Chaudhary, Zainab

    2016-01-01

    Current published data presents confusing results about the effects of platelet-rich fibrin on bone, and there is a need for studies that throw light on its effect. Our main objective therefore was to evaluate (by fractal analysis) osseous regeneration in extraction sockets with and without platelet-rich fibrin in a study with a substantial sample and a reliable technique to calibrate its effects on bone cells. We also assessed the soft tissue response. Thirty-four patients had their bilaterally impacted third molars (68 surgical sites) extracted in this split-mouth study, following which platelet-rich fibrin was placed in one of the sockets. Patients were followed up clinically and radiographically, and a pain score and fractal analysis were used to evaluate healing of soft tissue and bone, respectively. We conclude that platelet-rich fibrin improves healing of both soft and hard tissues. Although osseous healing did not differ significantly between the groups, healing of soft tissue as judged by the pain score was significantly better in the experimental group.

  5. [Anaphylactic shock caused by fibrin glue].

    Science.gov (United States)

    Orsel, I; Guillaume, A; Feiss, P

    1997-01-01

    We observed a case of anaphylactic shock in a 68-year-old woman after a nasal intramucosal injection of fibrin glue for telangiectasies therapy. The tests showed an allergy to aprotinin contained in the glue. In the previous years, glue and aprotinin had been administered to the patient several times for nasal bleeding.

  6. The Effects of Opaque and Clear Pit and Fissure Sealants on Infrared Laser Fluorescence Measurements

    Science.gov (United States)

    Bahrololoomi, Z.; Khodabakhsh, M.; Khaksar, Y.

    2014-01-01

    Statement of the Problem: The purpose of placing sealants is to inhibit caries by physical closure of the pits and fissures of teeth. A device named DIAGNOdent is useful in detecting occlusal caries by employing laser fluorescence (LF). However, there are contradictory results in the influence of sealants on LF measurements. Purpose: The aim of this study is to investigate the effects of two different types of fissure sealants on LF measurements. Materials and Method: In this in vitro study, 86 extracted permanent third molars were divided randomly into two groups and clear or opaque sealant was applied on the occlusal surfaces. Two examiners performed pre- and post-seal fluorescence measurements twice with one week interval by employing DIAGNOdent device. Finally, measured values were evaluated through the statistical paired t-test by means of SPSS 17 software. Results: The mean value of LF measurements increased significantly due to the application of clear sealant (p= 0.001) while the statistical changes in this measurement was negligible after applying opaque sealant (p= 0.311). Conclusion: Clear sealants increase the LF measured values but opaque sealants cause almost no changes. Therefore, DIAGNOdent device is not reliable for detecting caries beneath the clear sealant. PMID:24883342

  7. The Effects of Opaque and Clear Pit and Fissure Sealants on Infrared Laser Fluorescence Measurements

    Directory of Open Access Journals (Sweden)

    Bahrololoomi Z.

    2013-10-01

    Full Text Available Statement of the Problem: The purpose of placing sealants is to inhibit caries by physical closure of the pits and fissures of teeth. A device named DIAGNOdent is useful in detecting occlusal caries by employing laser fluorescence (LF. However, there are contradictory results in the influence of sealants on LF measurements. Purpose: The aim of this study is to investigate the effects of two different types of fissure sealants on LF measurements. Materials and Method: In this in vitro study, 86 extracted permanent third molars were divided randomly into two groups and clear or opaque sealant was applied on the occlusal surfaces. Two examiners performed pre and post-seal fluorescence measurements twice with one week interval by employing DIAGNOdent device. Finally, measured values were evaluated through the statistical paired t-test by means of SPSS 17 software. Results: The mean value of LF measurements increased significantly due to the application of clear sealant (p= 0.001 while the statistical changes in this measurement is negligible after applying opaque sealant (p= 0.311. Conclusion: Clear sealants increase the LF measured values but opaque sealants cause almost no changes. Therefore DIAGNOdent device is not reliable for detecting caries beneath the clear sealant.

  8. Toxicity of coal-tar and asphalt sealants to eastern newts, Notophthalmus viridescens.

    Science.gov (United States)

    Bommarito, Thomas; Sparling, Donald W; Halbrook, Richard S

    2010-09-01

    Between 1970 and 2000 the concentration of total polycyclic aromatic hydrocarbons (TPAH) in several lakes across the country increased whereas those of other persistent organic pollutants (POPs) tended to remain stable or declined. Urbanized watersheds experienced greater rises in TPAH concentration compared to non-urban lakes. Sources for urban PAHs include industrial wastes, vehicular exhausts and oil leaks and sealants from pavement surfaces. Both coal-tar and asphalt sealants are used to protect surfaces but runoff from surfaces coated with coal-tar can have mean concentrations of 3500 mg TPAHs kg(-1), much higher than runoff from asphalt-sealed or cement surfaces. Unaltered parent compounds of PAHs can have many lethal and sublethal toxic effects, but oxidation and UV radiation can alter the toxicity of these compounds, sometimes creating degradates that are many times more toxic than parent compounds. The purposes of this study were to determine if coal-tar sealants can be toxic to adult eastern newts (Notophthalmus viridescens) and to compare the toxicity of coal-tar sealant to that of asphalt sealant. Newts were exposed to sediments containing dried sealants ranging from 0 mg kg(-1) to 1500 mg kg(-1) under simultaneous exposure to UV radiation and visible light to determine concentration/response relationships. No significant mortality occurred with any treatment. Significant effects due to sealants included decreased righting ability and diminished liver enzyme activities. Coal-tar sealant was more effective in inducing these changes than was asphalt sealant.

  9. Toxicity of coal-tar and asphalt sealants to eastern newts, Notophthalmus viridescens

    Energy Technology Data Exchange (ETDEWEB)

    Bommarito, T.; Sparling, D.W.; Halbrook, R.S. [South Illinois University, Carbondale, IL (United States). Cooperative Wildlife Research Laboratory

    2010-09-15

    Between 1970 and 2000 the concentration of total polycyclic aromatic hydrocarbons (TPAH) in several lakes across the country increased whereas those of other persistent organic pollutants (POPs) tended to remain stable or declined. Urbanized watersheds experienced greater rises in TPAH concentration compared to non-urban lakes. Sources for urban PAHs include industrial wastes, vehicular exhausts and oil leaks and sealants from pavement surfaces. Both coal-tar and asphalt sealants are used to protect surfaces but runoff from surfaces coated with coal-tar can have mean concentrations of 3500 mg TPAHs kg{sup -1}, much higher than runoff from asphalt-sealed or cement surfaces. Unaltered parent compounds of PAHs can have many lethal and sublethal toxic effects, but oxidation and UV radiation can alter the toxicity of these compounds, sometimes creating degradates that are many times more toxic than parent compounds. The purposes of this study were to determine if coal-tar sealants can be toxic to adult eastern newts (Notophthalmus viridescens) and to compare the toxicity of coal-tar sealant to that of asphalt sealant. Newts were exposed to sediments containing dried sealants ranging from 0 mg kg{sup -1} to 1500 mg kg{sup -1} under simultaneous exposure to UV radiation and visible light to determine concentration/response relationships. No significant mortality occurred with any treatment. Significant effects due to sealants included decreased righting ability and diminished liver enzyme activities. Coal-tar sealant was more effective in inducing these changes than was asphalt sealant.

  10. Preparation of hybrid scaffold from fibrin and biodegradable polymer fiber.

    Science.gov (United States)

    Hokugo, Akishige; Takamoto, Tomoaki; Tabata, Yasuhiko

    2006-01-01

    A biodegradable hybrid scaffold was prepared from fibrin and poly(glycolic acid) (PGA) fiber. Mixed fibrinogen and thrombin solution homogeneously dispersed in the presence of various amounts (0, 1.5, 3.0, and 6.0mg) of PGA fiber was freeze-dried to obtain fibrin sponges with or without PGA fiber incorporation. By scanning electron microscopy observation, the fibrin sponges had an interconnected pore structure, irrespective of the amount of PGA fiber incorporated. PGA fiber incorporation enabled the fibrin sponges to significantly enhance their compression strength. In vitro cell culture studies revealed that the number of L929 fibroblasts initially attached was significantly larger for any fibrin sponge with PGA fiber incorporation than for the fibrin sponge without PGA fiber. The shrinkage of sponges after cell seeding was suppressed by fiber incorporation. It is possible that the shrinkage suppression of sponges maintains their intraspace, resulting in the superior cell attachment of a sponge with PGA fiber incorporation. After subcutaneous implantation into the backs of mice, the residual volume of a fibrin sponge with PGA fiber incorporation was significant compared with that of a fibrin sponge without PGA fiber. Larger number of cells infiltrated deep inside the fibrin sponges with PGA fiber incorporation implanted subcutaneously. It is concluded that the fibrin sponge reinforced by fiber incorporation is a promising three-dimensional scaffold of cells for tissue engineering.

  11. Monitoring of sound and carious surfaces under sealants over 44 months.

    Science.gov (United States)

    Fontana, M; Platt, J A; Eckert, G J; González-Cabezas, C; Yoder, K; Zero, D T; Ando, M; Soto-Rojas, A E; Peters, M C

    2014-11-01

    Although there is strong evidence for the effectiveness of sealants, one major barrier in sealant utilization is the concern of sealing over active caries lesions. This study evaluated detection and monitoring of caries lesions through a clear sealant over 44 mo. Sixty-four 7- to 10-year-old children with at least 2 permanent molars with International Caries Detection and Assessment System (ICDAS) scores 0-4 (and caries less than halfway through the dentin, radiographically) were examined with ICDAS, DIAGNOdent, and quantitative light-induced fluorescence (QLF) before sealant placement and 1, 12, 24, and 44 mo (except QLF) after. Bitewing radiographs were taken yearly. DIAGNOdent and QLF were able to distinguish between baseline ICDAS before and after sealant placement. There was no significant evidence of ICDAS progression at 12 mo, but there was small evidence of minor increases at 24 and 44 mo (14% and 14%, respectively) with only 2% ICDAS ≥ 5. Additionally, there was little evidence of radiographic progression (at 12 mo = 1%, 24 mo = 3%, and 44 mo = 9%). Sealant retention rates were excellent at 12 mo = 89%, 24 mo = 78%, and 44 mo = 70%. The small risk of sealant repair increased significantly as baseline ICDAS, DIAGNOdent, and QLF values increased. However, regardless of lesion severity, sealants were 100% effective at 12 mo and 98% effective over 44 mo in managing occlusal surfaces at ICDAS 0-4 (i.e., only 4 of 228 teeth progressed to ICDAS ≥ 5 associated with sealants in need of repair and none to halfway or more through the dentin, radiographically). This study suggests that occlusal surfaces without frank cavitation (ICDAS 0-4) that are sealed with a clear sealant can be monitored with ICDAS, QLF, or DIAGNOdent, which may aid in predicting the need for sealant repair.

  12. Monitoring of Sound and Carious Surfaces under Sealants over 44 Months

    Science.gov (United States)

    Fontana, M.; Platt, J.A.; Eckert, G.J.; González-Cabezas, C.; Yoder, K.; Zero, D.T.; Ando, M.; Soto-Rojas, A.E.; Peters, M.C.

    2014-01-01

    Although there is strong evidence for the effectiveness of sealants, one major barrier in sealant utilization is the concern of sealing over active caries lesions. This study evaluated detection and monitoring of caries lesions through a clear sealant over 44 mo. Sixty-four 7- to 10-year-old children with at least 2 permanent molars with International Caries Detection and Assessment System (ICDAS) scores 0-4 (and caries less than halfway through the dentin, radiographically) were examined with ICDAS, DIAGNOdent, and quantitative light-induced fluorescence (QLF) before sealant placement and 1, 12, 24, and 44 mo (except QLF) after. Bitewing radiographs were taken yearly. DIAGNOdent and QLF were able to distinguish between baseline ICDAS before and after sealant placement. There was no significant evidence of ICDAS progression at 12 mo, but there was small evidence of minor increases at 24 and 44 mo (14% and 14%, respectively) with only 2% ICDAS ≥ 5. Additionally, there was little evidence of radiographic progression (at 12 mo = 1%, 24 mo = 3%, and 44 mo = 9%). Sealant retention rates were excellent at 12 mo = 89%, 24 mo = 78%, and 44 mo = 70%. The small risk of sealant repair increased significantly as baseline ICDAS, DIAGNOdent, and QLF values increased. However, regardless of lesion severity, sealants were 100% effective at 12 mo and 98% effective over 44 mo in managing occlusal surfaces at ICDAS 0-4 (i.e., only 4 of 228 teeth progressed to ICDAS ≥ 5 associated with sealants in need of repair and none to halfway or more through the dentin, radiographically). This study suggests that occlusal surfaces without frank cavitation (ICDAS 0-4) that are sealed with a clear sealant can be monitored with ICDAS, QLF, or DIAGNOdent, which may aid in predicting the need for sealant repair. PMID:25248613

  13. Effect of Adhesive Application on Sealant Success: A Clinical Study of Fifth and Seventh Generation Adhesive Systems

    Directory of Open Access Journals (Sweden)

    Vaibhav Tandon

    2016-03-01

    Full Text Available Objectives: The aim of the study was to compare the effect of fifth and seventh generation bonding agent on sealant success.Materials and Methods: Sixty-four school children aged six to nine years received sealants in four permanent molars in a split mouth design, such that each patient received sealant in the first permanent molar with fifth generation bonding agent in one arch and seventh generation bonding agent in the other arch; contra-lateral molars were sealed with sealant alone. The evaluation was carried out at baseline, three months, six months and 12 months, according to the criteria by Feigal et al, in 2000. Chi- square test was used to analyze data at P<0.05 level of significance.Results: Statistically significant differences were found for sealant retention between fifth generation and sealant group, and fifth generation and seventh generation groups; whereas, no significant difference was found for sealant retention between seventh generation and sealant group at three, six and 12 months.Conclusion: As separate etch and rinse steps are not required for seventh generation bonding agents, and almost similar results were obtained for both sealant and seventh generation groups, it can be concluded that application of sealant along with a seventh generation bonding agent may enhance sealant success and can be used for caries prevention in preventive programs.Key words:  Pit and Fissure Sealants; Dental Caries; Adhesives

  14. Sealants in dentistry: outcomes of the ORCA saturday afternoon symposium 2007

    NARCIS (Netherlands)

    Splieth, C.H.; Ekstrand, K.R.; Alkilzy, M.; Clarkson, J.; Meyer-Lueckel, H.; Martignon, S.; Paris, S.; Pitts, N.B.; Rickets, D.N.; van Loveren, C.

    2010-01-01

    Sealants are a successful tool in caries prevention, but their role in preventive strategies after the caries decline has to be discussed. A survey of paediatric departments across Europe revealed that indications for pit and fissure sealants vary considerably, both nationally and internationally. E

  15. Qualification, Demonstration & Validation of Compliant Removers for Aircraft Sealants and Specialty Coatings ESTCP WP-0621

    Science.gov (United States)

    2010-06-01

    materials/ methods to approach sealant removal tasks consistently and effectively, depending upon situation. BUSINESS SENSITIVE 6 TECHNICAL APROACH ...Dem/Val 3 at New River MCAS - V-22 Osprey TECHNICAL APROACH BUSINESS SENSITIVE 5 TECHNICAL APROACH “Toolbox” Approach: Provide end users with...Demonstration Tests (UDRI) - Testing Protocols Parameter Test Test Method Sealant Removal Force Measuring Unit UDRI Proprietary Substrate Damage Potential

  16. Conversion degree, microhardness, microleakage and fluoride release of different fissure sealants.

    Science.gov (United States)

    Kuşgöz, Adem; Tüzüner, Tamer; Ulker, Mustafa; Kemer, Bariş; Saray, Onur

    2010-11-01

    The purpose of this study was to determine the degree of conversion (DC), microhardness, microleakage and fluoride release of a nano-filled resin based fissure sealant (Grandio Seal, GS) and compare it with an un-filled resin based fissure sealant (Clinpro, CL) and a glass-ionomer based fissure sealant (Fuji Triage, FT). Disk shaped specimens were prepared from tested fissure sealants to determine the DC, Vicker hardness (VHN) and fluoride release (FR). The DC and VHN of each material was evaluated after 24 h. The cumulative fluoride concentrations were evaluated at 1 h, 6 h, 12 h, 1, 7, 15 and 30 days. For microleakage evaluation, fissure sealants were applied to the etched and dried enamel surfaces of sound third molar teeth according to the manufacturer's instructions (n=10). After the thermocycling and mechanical loading procedures, microleakage assessments were carried out. Data were analyzed using one-way ANOVA followed by a post hoc Tukey test, the Kruskal Wallis and Mann-Whitney U test (p GS (55.02%) > CL (%51.10) (p FT > CL (p<0.05). FT exhibited significantly higher microleakage scores compared to the CL and GS sealants (p<0.05). The FR of FT was significantly greater than CL and GS (p<0.05). Nano-filled resin based sealant can be used as an alternative to other fissure sealant materials because of its superior hardness results and feasible sealing ability.

  17. Long-term performance of resin based fissure sealants placed in a general dental practice.

    NARCIS (Netherlands)

    Hevinga, M.; Opdam, N.J.M.; Bronkhorst, E.M.; Truin, G.J.; Huysmans, M.C.D.N.J.M.

    2010-01-01

    OBJECTIVES: The aim of the present retrospective study was to evaluate the long-term performance of resin based fissure sealants applied in a general dental practice. METHODS: Regularly attending patients visiting the practice between July 2006 until November 2007 and who had received sealants befor

  18. Biodegradable-Polymer-Blend-Based Surgical Sealant with Body-Temperature-Mediated Adhesion.

    Science.gov (United States)

    Behrens, Adam M; Lee, Nora G; Casey, Brendan J; Srinivasan, Priya; Sikorski, Michael J; Daristotle, John L; Sandler, Anthony D; Kofinas, Peter

    2015-12-22

    The development of practical and efficient surgical sealants has the propensity to improve operational outcomes. A biodegradable polymer blend is fabricated as a nonwoven fiber mat in situ. After direct deposition onto the tissue of interest, the material transitions from a fiber mat to a film. This transition promotes polymer-substrate interfacial interactions leading to improved adhesion and surgical sealant performance.

  19. Pit and fissure sealants for preventing dental decay in the permanent teeth of children and adolescents

    DEFF Research Database (Denmark)

    Ahovuo-Saloranta, Anneli; Hiiri, Anne; Nordblad, Anne

    2008-01-01

    BACKGROUND: Although pit and fissure sealants are effective in preventing caries, their efficacy may be related to the caries prevalence in the population. OBJECTIVES: The primary objective of this review was to evaluate the caries prevention of pit and fissure sealants in children and adolescent...

  20. Caries detection methods : Can they aid decision making for invasive sealant treatment?

    NARCIS (Netherlands)

    Pereira, AC; Verdonschot, EH; Huysmans, MCDNJM

    2001-01-01

    The decision to place sealants is a difficult one, and it has been suggested that in a low risk population it may be efficient to wait until caries is detected in the fissure. An invasive sealant technique with fissure preparation may then be indicated. The diagnostic method used in the indication o

  1. The effect of bonding agents on the microleakage of sealant following contamination with saliva

    Directory of Open Access Journals (Sweden)

    Askarizadeh Nahid

    2008-06-01

    Full Text Available Aims : An issue of concern in dentistry is the inadequacy of adhesion and proper sealing following restoration of a tooth, which can lead to marginal leakage. The aim of this study was to evaluate the effect of a bonding agent on the microleakage of a sealant material following contamination with saliva. Materials and Methods : In this experimental research, 48 sound premolars were divided into two groups. The first group received sealant without bonding and the other group was given sealant with bonding. After prophylaxis, the occlusal surfaces were etched with 37% phosphoric acid gel and the teeth were then placed in fresh human saliva for l0 s. Following this, in the first group fissure sealant (Kerr was applied directly and cured; for the second group sealant was placed and cured after bonding (Single Bond; 3M. All samples were thermocycled (500 cycles; between 5°C and 55°C; dwell time of 30 s. Silver nitrate was used as the leakage tracer. The teeth were sectioned. Microleakage evaluation was made by stereomicroscope at 40x magnification and the results were evaluated with the Mann-Whitney U test. Results : In the group that received sealant without bonding extensive microleakage was seen; placement of sealant with bonding significantly reduced microleakage. Conclusion : In the presence of contamination with saliva, use of bonding under the fissure sealant can reduce microleakage

  2. Fissure sealant materials: Wear resistance of flowable composite resins

    Science.gov (United States)

    Asefi, Sohrab; Eskandarion, Solmaz; Hamidiaval, Shadi

    2016-01-01

    Background. Wear resistance of pit and fissure sealant materials can influence their retention. Wear characteristics of sealant materials may determine scheduling of check-up visits. The aim of this study was to compare wear resistance of two flowable composite resins with that of posterior composite resin materials. Methods. Thirty-five disk-shaped specimens were prepared in 5 groups, including two flowable composite resins (Estelite Flow Quick and Estelite Flow Quick High Flow), Filtek P90 and Filtek P60 and Tetric N-Ceram. The disk-shaped samples were prepared in 25-mm diameter by packing them into a two-piece aluminum mold and then light-cured. All the specimens were polished for 1minute using 600-grit sand paper. The samples were stored in distilled water at room temperature for 1 week and then worn by two-body abrasion test using "pin-on-disk" method (with distilled water under a 15-Nload at 0.05 m/s, for a distance of 100 meter with Steatite ceramic balls antagonists). A Profilometer was used for evaluating the surface wear. Data were analyzed with the one-way ANOVA. Results. Estelite Flow Quick exhibited 2708.9 ± 578.1 μm2 and Estelite Flow Quick High Flow exhibited 3206 ± 2445.1 μm2of wear but there were no significant differences between the groups. They demonstrated similar wear properties. Conclusion. Estelite flowable composite resins have wear resistance similar to nano- and micro-filled and micro-hybrid composite resins. Therefore, they can be recommended as pit and fissure sealant materials in the posterior region with appropriate mechanical characteristics. PMID:27651887

  3. Fissure sealant materials: Wear resistance of flowable composite resins.

    Science.gov (United States)

    Asefi, Sohrab; Eskandarion, Solmaz; Hamidiaval, Shadi

    2016-01-01

    Background. Wear resistance of pit and fissure sealant materials can influence their retention. Wear characteristics of sealant materials may determine scheduling of check-up visits. The aim of this study was to compare wear resistance of two flowable composite resins with that of posterior composite resin materials. Methods. Thirty-five disk-shaped specimens were prepared in 5 groups, including two flowable composite resins (Estelite Flow Quick and Estelite Flow Quick High Flow), Filtek P90 and Filtek P60 and Tetric N-Ceram. The disk-shaped samples were prepared in 25-mm diameter by packing them into a two-piece aluminum mold and then light-cured. All the specimens were polished for 1minute using 600-grit sand paper. The samples were stored in distilled water at room temperature for 1 week and then worn by two-body abrasion test using "pin-on-disk" method (with distilled water under a 15-Nload at 0.05 m/s, for a distance of 100 meter with Steatite ceramic balls antagonists). A Profilometer was used for evaluating the surface wear. Data were analyzed with the one-way ANOVA. Results. Estelite Flow Quick exhibited 2708.9 ± 578.1 μm(2) and Estelite Flow Quick High Flow exhibited 3206 ± 2445.1 μm(2)of wear but there were no significant differences between the groups. They demonstrated similar wear properties. Conclusion. Estelite flowable composite resins have wear resistance similar to nano- and micro-filled and micro-hybrid composite resins. Therefore, they can be recommended as pit and fissure sealant materials in the posterior region with appropriate mechanical characteristics.

  4. Randomized trial on fluorides and sealants for fissure caries prevention

    OpenAIRE

    Lin, HC; Liu, BY; Lo, ECM; Chu, CH

    2012-01-01

    To investigate the effectiveness of topical fluorides in preventing fissure caries, we conducted a randomized controlled trial with parallel groups. In total, 501 children (1,539 molars, 3,078 sites), mean age 9.1 years, who had at least one sound permanent first molar with deep fissures or fissures with signs of early caries were recruited. They were randomly allocated among four groups: (1) resin sealant, single placement; (2) 5% NaF varnish, semi-annual application; (3) 38% silver diamine ...

  5. Bonding durability of dental sealants to deciduous and permanent teeth

    OpenAIRE

    MOURA, Sandra Kiss; Lemos,Letícia Vargas Freire Martins; Myszkovisk, Suellen; Provenzano, Maria Gisette Arias; Balducci,Ivan; Myaki, Silvio Issao [UNESP

    2014-01-01

    AIM: To evaluate the bonding durability of materials used as sealants on different dentitions. METHODS: Deciduous (D) and permanent (P) molars were divided into four groups (n=5) and sealed with Fluroshield (F) and OptiBond FL (FL). Blocks of composite resin were built, stored in distilled water (24 h/37 °C), sectioned into 0.8 mm2 and tested at tensile (0.5 mm/min) after 24 h and 6 months. The fractures were observed and classified into adhesive, cohesive or mixed types. The data were a...

  6. Effect of an Antibacterial Monomer on the Antibacterial Activity of a Pit-and-Fissure Sealant

    Science.gov (United States)

    Dong, Yan; Zhang, Ling; Sun, Xiang; Liu, Zhengya; Guo, Huihui; Huang, Li; Chen, Jihua

    2016-01-01

    Resin-based pit-and-fissure sealants are often used to form a barrier on the occlusal surface of molars to treat caries lesions; however, bacteria can remain in the pit and fissures without detection, increasing the risk of secondary caries. Sealants with antimicrobial properties or microbial repellent actions might be advantageous. The aim of this study was to assess the inhibitory effect of a 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB)-incorporated sealant against Streptococcus mutans. MAE-DB (4% wt) was incorporated into a commercially available sealant, Eco-S resin-based pit-and-fissure sealant (Vericom Co., Ltd., Korea); a sealant without MAE-DB served as a negative control, and Clinpro™ Sealant (3M™ ESPE™), a fluoride-releasing resin, was used as a commercial control. The effects of the cured sealants and their eluents on the growth of S. mutans were determined according to colony-forming unit counts and metabolic tests. The effects of the cured sealants on the adherence and membrane integrity of S. mutans were investigated using confocal laser-scanning microscopy (CLSM) in conjunction with fluorescent indicators. Compared with the negative control and commercial control, the cured MAE-DB-incorporated pit-and-fissure sealant exhibited a significant inhibitory effect on the growth of S. mutans (P < 0.05), whereas the eluents did not show any detectable antibacterial activity. The commercial control also showed no detectable bactericidal activity. Moreover, the aged experimental material retained its property of contact inhibition of biofilm formation. The fluorescence analysis of CLSM images demonstrated that the cured MAE-DB-incorporated sealant could hamper the adherence of S. mutans and exert a detrimental effect on bacterial membrane integrity. The incorporation of MAE-DB can render a pit-and-fissure sealant with contact antibacterial activity after polymerization via influencing the growth, adherence, and membrane integrity of S

  7. Identification of fibrin clot-bound plasma proteins

    NARCIS (Netherlands)

    S. Talens (Simone); F.W.G. Leebeek (Frank); J.A.A. Demmers (Jeroen); D.C. Rijken (Dingeman)

    2012-01-01

    textabstractSeveral proteins are known to bind to a fibrin network and to change clot properties or function. In this study we aimed to get an overview of fibrin clot-bound plasma proteins. A plasma clot was formed by adding thrombin, CaCl2 and aprotinin to citrated platelet-poor plasma and unbound

  8. A 1-year clinical evaluation of fissure sealants on permanent first molars

    Directory of Open Access Journals (Sweden)

    Nupur Ninawe

    2012-01-01

    Full Text Available Objective : To evaluate and compare the retention, marginal discoloration, surface texture and anatomical form of pit and fissure sealants. Materials and Methods : Thirty children between the ages of 6 and 10 years, who were attending the school health program regularly, had participated in the study. A split-mouth design was used in which the two fissure sealants (Helioseal-F and Glass ionomer Fuji VII were randomly placed in 60 matched contralateral pairs of permanent molar teeth. Sealants were rated by a single trained and calibrated examiner using mouth mirrors and probes following the US Public Health Service criteria. The sealants were evaluated at 3 months, 6 months and 1 year intervals. Results : The data obtained for retention, marginal discoloration, surface texture and anatomical form of pit and fissure sealants were tabulated and compared statistically using the Chi-square test of significance. Conclusion : The Helioseal-F sealant was better than the Glass ionomer Fuji VII sealant with respect to retention, anatomical form and surface texture. Both the materials showed similar results with respect to marginal discoloration.

  9. Selective removal of composite sealants with near-ultraviolet laser pulses of nanosecond duration.

    Science.gov (United States)

    Louie, Tiffany M; Jones, Robert S; Sarma, Anupama V; Fried, Daniel

    2005-01-01

    It is often necessary to replace pit and fissure sealants and composite restorations. This task is complicated by the necessity for complete removal of the remaining composite to enable suitable adhesion of new composite. Previous studies have shown that 355-nm laser pulses from a frequency-tripled Nd:YAG laser can selectively remove residual composite after orthodontic bracket removal on enamel surfaces. Our objective is to determine if such laser pulses are suitable for selective removal of composite pit and fissure sealants and restorations. Optical coherence tomography is used to acquire optical cross sections of the occlusal topography nondestructively before sealant application, after sealant application, and after sealant removal. Thermocouples are used to monitor the temperature in the pulp chamber during composite removal under clinically relevant ablation rates, i.e., 30 Hz and 30 mJ/pulse. At an irradiation intensity of 1.3 J/cm2, pit and fissure sealants are completely removed without visible damage to the underlying enamel. At intensities above 1.5 J/cm2, incident laser pulses remove the resin layer while at the same time preferentially etching the surface of the enamel. Temperature excursions in the pulp chamber of extracted teeth are limited to less than 5 degrees C if air-cooling is used during the rapid removal (1 to 2 min) of sealants, water-cooling is not necessary. Selective removal of composite restorative materials is possible without damage to the underlying sound tooth structure.

  10. Comparison of various concentrations of tricalcium phosphate nanoparticles on mechanical properties and remineralization of fissure sealants.

    Directory of Open Access Journals (Sweden)

    Sara Tavassoli-Hojjati

    2014-08-01

    Full Text Available The aim of this study was to investigate the mechanical properties (flexural strength, micro-shear bond strength and remineralizing potential of fissure sealants by adding various concentrations of β-tricalcium phosphate nanoparticles.This in-vitro study consisted of five experimental groups containing prepared nano-fisssure sealants (1-5 wt.% β-TCP nanoparticles and two control groups containing a prepared and a commercial fissure sealant. Flexural/micro-shear bond strength values were measured using Zwick test machine. Cavities on sixty healthy premolar teeth were filled with the fissure sealants containing 0-5 wt.% of nano β-TCP. The samples were assessed for remineralization under scanning electron microscopy (SEM and EDAX. Kolmogorov-Smirnov test, One-way ANOVA and Tukey's Post Hoc analysis/HSD were used to analyze the data.There was no significant difference between the flexural strengths/elastic modulus of the 0-5 wt.% nano β-TCP groups (p>0.05. The average flexural strength/elastic modulus of the prepared fissure sealant group (0% was significantly higher than the commercial fissure sealant group (Clinpro (p0.05. Examining the samples under SEM showed a significant increase in thickness of the intermediate layer with increasing concentrations of β-TCP nanoparticles (p<0.05.Addition of 1-5 wt.% β-TCP nanoparticles to the fissure sealants significantly increased the remineralization potential without affecting the mechanical properties.

  11. Laboratory testing of novel sealant for leaky wells

    Energy Technology Data Exchange (ETDEWEB)

    Bibic, O. [EnCana Corp., Calgary, AB (Canada); Polikar, M. [Alberta Univ., Edmonton, AB (Canada); Boyd, J. [Seal-MastR Systems Ltd., Clagary, AB (Canada)

    2006-07-01

    Abandoned gas wells in western Canada often leak gas to the surface. An experimental study was conducted in order to determine the possibility of using an asphalt-in-water emulsion as sealant for abandoned leaky wells. This is considered a more efficient and cost-effective method to the conventional cement grout barrier. The study objective was to test this new sealant technology proposed by Seal-MastR and to examine the effect of those parameters affecting the placement and fixing of an emulsion in the near well bore matrix, thereby hindering the flow of water or gas. Two types of experiments were conducted as part of this study. The mechanism of the emulsion penetration into the porous matrix was studied making observations of the process in a transparent core holder. The process was investigated using a steel core holder that allows injection of the emulsion at actual well pressures. It was concluded that the asphalt-in-water emulsion technology can be used with success for sealing abandoned gas-leaking wells or water-producing formations. The study also proposed recommendations for field application and suggested areas for further research to improve the process, such as finding the optimum concentration for the surfactant solution pre-flush that would yield the highest penetration, and testing the emulsion in oil-wet or partially oil-wet formation. 4 refs., 3 tabs., 19 figs.

  12. Stopping duct quacks: Longevity of residential duct sealants

    Energy Technology Data Exchange (ETDEWEB)

    Sherman, Max H.; Walker, Iain S.; Dickerhoff, Darryl J.

    2000-08-01

    Duct leakage has been identified as a major source of energy loss in residential buildings. Most duct leakage occurs at the connections to registers, plenums or branches in the duct system. At each of these connections a method of sealing the duct system is required. Typical sealing methods include tapes or mastics applied around the joints in the system. Field examinations of duct systems have shown that these seals tend to fail over time periods ranging from days to years. We have used several test methods over the last few years to evaluate the longevity of duct sealants when subjected to temperatures and pressures representative of those found in the field. Traditional cloth duct tapes have been found to significantly under-perform other sealants and have been banned from receiving duct tightness credits in California's energy code (California Energy Commission 1998). Our accelerated testing apparatus has been redesigned since its first usage for improved performance. The methodology is currently under consideration by the American Society for Testing and Materials (ASTM) as a potential new test method. This report will summarize the set of measurements to date, review the status of the test apparatus and test method, and summarize the applications of these results to codes and standards.

  13. Predictive factors for anterior chamber fibrin formation after vitreoretinal surgery

    Directory of Open Access Journals (Sweden)

    Leonardo Provetti Cunha

    2014-04-01

    Full Text Available Purpose: The aim of this study was to investigate possible predictive factors related to anterior chamber fibrin formation after vitreoretinal surgery in a large series of patients. Methods: The data of 185 eyes of 185 patients submitted to vitreoretinal surgery was reviewed. The following variables were evaluated: the postoperatively presence of fibrin, age, diabetes mellitus, the vitrectomy system gauge (20, 23 or 25 gauge, the type of vitreous substitute, the influence of prior surgical procedures and the combination with cataract extraction. To evaluate predictive factors for anterior chamber fibrin formation, univariate analysis was performed. A multivariate stepwise logistic regression model was adjusted to investigate factors associated with fibrin formation (p<0.05. Results: Fibrinoid anterior chamber reaction was found in 12 (6.4% patients. For multivariate logistic regression analysis, balanced salt solution (BSS, the chance of fibrin occurrence was 5 times greater (odds ratio 4.83, CI 95% 1.302 - 17.892; p=0.019, while combination with phacoemulsification increased the chance of fibrin formation by 20 times (odds ratio 20, CI 95% 2.480 - 161.347; p=0.005. No significant difference was found regarding other variables. Conclusion: Anterior chamber fibrin formation is an unwanted complication after vitreoretinal surgery. Factors such as combined performance of phacoemulsification and the use of balanced salt solution as a vitreous substitute may predispose the occurrence of this complication.

  14. Role of plasma-rich fibrin in oral surgery

    Science.gov (United States)

    Kumar, K. Retna; Genmorgan, K.; Abdul Rahman, S. M.; Rajan, M. Alaguvel; Kumar, T. Arul; Prasad, V. Srinivas

    2016-01-01

    Platelet-rich fibrin (PRF) is a fibrin meshwork, in which platelet cytokines, growth factors, and cells are entrapped and discharged after a period and can serve as a resorbable film. PRF is the next generation of platelet concentrates equipped to improve arrangement without biochemical blood handling; PRF is an evolution of the fibrin adhesive, which is widely used in the oral surgery. The guidelines of this innovation depend on concentrating platelets and growth factors in a plasma medium, and initiating them in a fibrin gel, keeping in mind the end goal to enhance the healing of wounds. Maxillary bone loss requires numerous regenerative techniques: as a supplement to the procedures of tissue regeneration, a platelet concentrate called PRF was tested for the 1st time in France by Dr. Choukroun. This article enriches the benefits and role of plasma-rich fibrin in oral surgery. Platelet-concentrate fibrin is an evolution of the fibrin glue, which is widely used in the oral surgery. PMID:27829743

  15. Role of plasma-rich fibrin in oral surgery

    Directory of Open Access Journals (Sweden)

    K Retna Kumar

    2016-01-01

    Full Text Available Platelet-rich fibrin (PRF is a fibrin meshwork, in which platelet cytokines, growth factors, and cells are entrapped and discharged after a period and can serve as a resorbable film. PRF is the next generation of platelet concentrates equipped to improve arrangement without biochemical blood handling; PRF is an evolution of the fibrin adhesive, which is widely used in the oral surgery. The guidelines of this innovation depend on concentrating platelets and growth factors in a plasma medium, and initiating them in a fibrin gel, keeping in mind the end goal to enhance the healing of wounds. Maxillary bone loss requires numerous regenerative techniques: as a supplement to the procedures of tissue regeneration, a platelet concentrate called PRF was tested for the 1st time in France by Dr. Choukroun. This article enriches the benefits and role of plasma-rich fibrin in oral surgery. Platelet-concentrate fibrin is an evolution of the fibrin glue, which is widely used in the oral surgery.

  16. Retention and Effectiveness of Dental Sealant After Twelve Months in Iranian Children

    Directory of Open Access Journals (Sweden)

    Rafatjou

    2013-12-01

    Full Text Available Background Pit-and-fissure sealants are safe and effective ways to prevent dental caries and are considered as a part of an overall caries-preventive strategy. Dental caries are a public health problem and the most common intraoral disease affecting mankind. It is an infectious transmissible disease, with the child patient being at the highest risk. Objectives The purpose of this study was to evaluate the retention rates and effectiveness of occlusal sealants in children in Hamadan, Iran. Materials and Methods A total of 118 sealed first permanent molar teeth in 52 students (mean age, 8 years;male, 48% were evaluated for fissure sealant retention and occlusal caries status. All teeth were examined 12 months after application of sealants. Data were collected and evaluated by survival analyses methods for age at placement, patient sex, decayed/missing/filled teeth (DMFT index, fluoride history, toothbrush, tooth position in arch, and refer to dentistry. Results The overall success rate with pit-and-fissure sealant was 68.6%; in addition, 38.9% of the seals were completely retained, 38.9% partially lost, and 10.2% completely lost. There were no signs of carious lesions in 69.6% of the teeth. The factors associated with an increased risk of failure included female sex (P = 0.001 and no history of fluoride use (P < 0.01. There were no significant association between the results and patient age, tooth position in the arch, DMFT index, toothbrush, and refer to dentistry. Conclusions Although pit-and-fissure sealants are effective methods for preventing tooth caries, the low success rate of fissure sealants in current study indicated that dental sealant need to be implemented more carefully and follow-up programs are advisable.

  17. OMNEX surgical sealant in the extracorporeal repair of renal artery aneurysms.

    Science.gov (United States)

    Garcia-Roig, Michael; Gorin, Michael A; Castellan, Miguel; Ciancio, Gaetano

    2011-11-01

    OMNEX (Ethicon, Inc.; Somerville, NJ) is a cyanoacrylate-based synthetic surgical sealant. To date, the use of OMNEX has only been described in a limited number of vascular surgery procedures. We present the cases of two patients who underwent successful extracorporeal renal artery aneurysm repair with the aid of OMNEX sealant. This report is the first evidence to suggest the utility of OMNEX in renovascular surgery.

  18. Relation Between PAHs and Coal-Tar-Based Pavement Sealant in Urban Environments (Invited)

    Science.gov (United States)

    Mahler, B. J.; van Metre, P. C.

    2010-12-01

    Since 2003, coal-tar-based sealant products have come under increased scrutiny as a source of PAHs in urban environments. Sealant (or sealcoat) is the black, shiny substance often applied to asphalt pavement, in particular parking lots and driveways, for esthetic and maintenance purposes. Coal-tar-based sealant, one of the two primary pavement sealant types on the market, typically is 20-35 percent coal-tar pitch, a known carcinogen that is more than 50 percent polycyclic aromatic hydrocarbons (PAHs). The PAH content of the coal-tar-based sealant product is about 1,000 times that of a similar, asphalt-based product, on average. This difference is reflected in regional differences in sealant use and PAH concentrations in pavement dust. In the central and eastern U.S., where the coal-tar-based formulation is prevalent, ΣPAH in mobile particles from sealed pavement have been shown to be about 1,000 times higher than in the western U.S., where the asphalt-based formulation is prevalent (the median ΣPAH concentrations are 2,200 mg/kg in the central and eastern U.S. and 2.1 mg/kg in the western U.S.). Source apportionment modeling indicates that, in the central and eastern U.S., particles from sealed pavement are contributing the majority of the PAHs in recently deposited (post-1990) lake sediment, with implications for ecological health, and that coal-tar-based sealant is the primary cause of upward trends in PAHs in U.S. urban lakes. From the standpoint of human health, research indicates that mobile particles from parking lots with coal-tar-based sealant are tracked indoors, resulting in elevated PAH concentrations in house dust. Coal-tar-based sealcoat being applied to an asphalt parking lot at the University of Texas Pickle Research Center.

  19. Effect of LED curing on the marginal leakage of a pit and fissure sealant

    OpenAIRE

    Pineda Mejia, Martha Elena; Magister en Estomatología. Profesor Principal. UNMSM. Dpto. Estomatología Rehabilitadora. Facultad Odontología. UNMSM.; Gloria Zevallos, Waldo; Dpto. Estomatología Rehabilitadora. Facultad Odontología. UNMSM.; Abuhadba Hoyos, Tulio; Dpto. Estomatología Rehabilitadora. Facultad Odontología. UNMSM.

    2014-01-01

    The purpose of this study was to evaluate in vitro the curing effect of a light-emitting diode LED unit and conventional halogen light on the marginal leakage of a pit and fissure sealant. We used 40 caries-free permanent premolars recently extracted, which were randomly divided into two groups (n = 20), sealed with a resin based fissure sealant, Helioseal F (Ivoclar Vivadent), according to the manufacturer patterns, then photopolymerized using either L.E.D. light or conventional halogen ligh...

  20. CDC Vital Signs–Dental Sealants Prevent Cavities

    Centers for Disease Control (CDC) Podcasts

    2016-10-18

    This podcast is based on the October 2016 CDC Vital Signs report. Dental sealants, applied soon after a child's permanent molars come in, can protect against cavities for up to nine years. Applying sealants in schools for low-income children could save millions in dental treatment costs.  Created: 10/18/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/18/2016.

  1. Microtensile bond strength of sealant and adhesive systems applied to occlusal primary enamel

    OpenAIRE

    Ramires-Romito, ACD; Reis, A; Loguercio, AD; Hipolito, VD; de Goes, MF; Singer, JD; Grande, RHM

    2007-01-01

    Purpose: To compare the microtensile bond strength (mu TBS) of a self-etch adhesive system (OptiBond Solo Self-Etch Adhesive System), two total etch adhesive system (OptiBond FL; OptiBond Solo), and a conventional sealant (Clinpro) applied to the occlusal surface of primary molars under saliva contamination. Methods: Sealant and adhesive systems were applied under manufacturers' specifications with or without previous saliva contamination. After storage in distilled water at 37 degrees C for ...

  2. Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment.

    Science.gov (United States)

    Obonai, Toshifumi; Fuchigami, Hirobumi; Furuya, Fumiaki; Kozuka, Naoyuki; Yasunaga, Masahiro; Matsumura, Yasuhiro

    2016-01-01

    The diagnosis of early and aggressive types of cancer is important for providing effective cancer therapy. Cancer-induced fibrin clots exist only within lesions. Previously, we developed a monoclonal antibody (clone 102-10) that recognizes insoluble fibrin but not fibrinogen or soluble fibrin and confirmed that fibrin clots form continuously in various cancers. Here, we describe the development of a Fab fragment probe of clone 102-10 for tumour imaging. The distribution of 102-10 Fab was investigated in genetically engineered mice bearing pancreatic ductal adenocarcinoma (PDAC), and its effect on blood coagulation was examined. Immunohistochemical and ex vivo imaging revealed that 102-10 Fab was distributed selectively in fibrin clots in PDAC tumours 3 h after injection and that it disappeared from the body after 24 h. 102-10 Fab had no influence on blood coagulation or fibrinolysis. Tumour imaging using anti-fibrin Fab may provide a safe and effective method for the diagnosis of invasive cancers by detecting fibrin clots in tumour stroma.

  3. A comparison between three different pit and fissure sealants with regard to marginal integrity

    Science.gov (United States)

    Fernandes, Kristlee Sabrin; Chalakkal, Paul; de Ataide, Ida de Noronha; Pavaskar, Rajdeep; Fernandes, Precylia Philo; Soni, Harleen

    2012-01-01

    Aim: The aim of this study is to compare the occurrence of enamel fractures, sealant fractures and marginal fissures after placement of three sealants: Helioseal F, Conseal F and Clinpro. Materials and Methods: Thirty individuals between 13 and 15 years of age, diagnosed with pit and fissure caries by visual and DIAGNOdent examination, were chosen for sealant placement on their mandibular molars. The sealants were placed at random, after which, impressions were made with polyvinyl siloxane and casts were fabricated. Dies were prepared, each of which were sputter coated with gold in order to be examined under a scanning electron microscope. The following morphologies were analyzed from dies from each of the sealant groups: Continuous margins, sealant fractures, marginal fissures and enamel fractures. After six months, they were recalled for impression making. Dies were prepared and microscopically analyzed as mentioned. Based on the time of evaluation, there were two groups: Initial group (soon after placement) and final group (after six months). Statistical analysis was done using the paired ‘t’ test and One-way analysis of variance (ANOVA). Results and Conclusions: Clinpro had the greatest fracture resistance, followed by Conseal F and Helioseal F. The occurrence of marginal fissure was found to be least with Clinpro. PMID:22557813

  4. Relationship between DIAGNOdent values and sealant penetration depth on occlusal fissures.

    Science.gov (United States)

    Rodrigues, Jonas A; Diniz, Michele B; Hug, Isabel; Cordeiro, Rita C L; Lussi, Adrian

    2010-12-01

    The aim of this in vitro study was to evaluate the relationship between laser fluorescence values and sealant penetration depth on occlusal fissures. One hundred and sixty-six permanent molars were selected and divided into four groups, which were each treated using a different sealant (two clear and two opaque). The teeth were independently measured twice by two experienced dentists using two laser fluorescence devices-DIAGNOdent (LF and LFpen)-before and after sealing, and then thermoclycled. After measuring, the teeth were histologically prepared and assessed for caries extension. Digital photographs of the cut sealed sites were assessed, and the sealant penetration depth was measured. All 166 sites were measured by one of the examiners taking as limits the outer and inner surface of the sealant into the fissure. For each device (LF and LFpen) and each group, the difference between the values at baseline and after sealing was plotted against the sealant penetration depth and scatter plots were provided. It could be observed that most of the points were concentrated around the zero line, for both LF and LFpen in the four groups. In conclusion, there is no relation between changes in DIAGNOdent values and increasing of depth sealant penetration within the occlusal fissures.

  5. A comparison between three different pit and fissure sealants with regard to marginal integrity

    Directory of Open Access Journals (Sweden)

    Kristlee Sabrin Fernandes

    2012-01-01

    Full Text Available Aim: The aim of this study is to compare the occurrence of enamel fractures, sealant fractures and marginal fissures after placement of three sealants: Helioseal F, Conseal F and Clinpro. Materials and Methods: Thirty individuals between 13 and 15 years of age, diagnosed with pit and fissure caries by visual and DIAGNOdent examination, were chosen for sealant placement on their mandibular molars. The sealants were placed at random, after which, impressions were made with polyvinyl siloxane and casts were fabricated. Dies were prepared, each of which were sputter coated with gold in order to be examined under a scanning electron microscope. The following morphologies were analyzed from dies from each of the sealant groups: Continuous margins, sealant fractures, marginal fissures and enamel fractures. After six months, they were recalled for impression making. Dies were prepared and microscopically analyzed as mentioned. Based on the time of evaluation, there were two groups: Initial group (soon after placement and final group (after six months. Statistical analysis was done using the paired ′t′ test and One-way analysis of variance (ANOVA. Results and Conclusions: Clinpro had the greatest fracture resistance, followed by Conseal F and Helioseal F. The occurrence of marginal fissure was found to be least with Clinpro.

  6. Low-cost glass ionomer cement as ART sealant in permanent molars: a randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Daniela HESSE

    2015-01-01

    Full Text Available Clinical trials are normally performed with well-known brands of glass ionomer cement (GIC, but the cost of these materials is high for public healthcare in less-affluent communities. Given the need to research cheaper materials, it seems pertinent to investigate the retention rate of a low-cost GIC applied as atraumatic restorative treatment (ART sealants in two centers in Brazil. Four hundred and thirty-seven 6-to-8-year-old schoolchildren were selected in two cities in Brazil. The children were randomly divided into two groups, according to the tested GIC applied in the first permanent molars. The retention rate was evaluated after 3, 6 and 12 months. Kaplan-Meier survival analysis and the log-rank test were performed. The variables were tested for association with sealant longevity, using logistic regression analyses (α = 5%. The retention rate of sealants after 12 months was 19.1%. The high-cost GIC brand presented a 2-fold-more-likely-to-survive rate than the low-cost brand (p < 0.001. Significant difference was also found between the cities where the treatments were performed, in that Barueri presented a higher sealant survival rate than Recife (p < 0.001. The retention rate of a low-cost GIC sealant brand was markedly lower than that of a well-known GIC sealant brand.

  7. LIQUIDARMOR CM Flashing and Sealant, High Impact Technology Demonstration

    Energy Technology Data Exchange (ETDEWEB)

    Hun, Diana E [ORNL; Bhandari, Mahabir S [ORNL

    2016-12-01

    Air leakage is responsible for about 1.1 quads of energy or 6% of the total energy used by commercial buildings in the US. Consequently, infiltration and exfiltration are among the largest envelope-related contributors to the heating, ventilation, and air conditioning loads in commercial buildings. New air sealing technologies have recently emerged that aim to improve the performance of air barrier systems by simplifying their installation procedure. LIQUIDARMORTM CM Flashing and Sealant is an example of these new advanced material technologies. This technology is a spray-applied sealant and liquid flashing and can span gaps that are up to ¼ in. wide without a supporting material. ORNL verified the performance of LIQUIDARMORTM CM with field tests and energy simulations from a building in which LIQUIDARMORTM CM was one of components of the air barrier system. The Homeland Security Training Center (HTC) at the College of DuPage in Glen Ellyn, IL, served as the demonstration site. Blower door test results show the average air leakage rate in the demonstration site to be 0.15 cfm/ft2 at 1.57 psf, or 63% lower than the 0.4 cfm at 1.57 psf specified in the 2015 International Energy Conservation Code (IECC). According to simulation results, HTC lowered its annual heating and cooling cost by about $3,000 or 9% compared to a similar building that lacked an air barrier system. This demonstration project serves as an example of the level of building envelope airtightness that can be achieved by using air barrier materials that are properly installed, and illustrates the energy and financial savings that such an airtight envelope could attain.

  8. Altered plasma fibrin clot properties in essential thrombocythemia.

    Science.gov (United States)

    Małecki, Rafał; Gacka, Małgorzata; Kuliszkiewicz-Janus, Małgorzata; Jakobsche-Policht, Urszula; Kwiatkowski, Jacek; Adamiec, Rajmund; Undas, Anetta

    2016-01-01

    Patients with increased thromboembolic risk tend to form denser fibrin clots which are relatively resistant to lysis. We sought to investigate whether essential thrombocythemia (ET) is associated with altered fibrin clot properties in plasma. Ex vivo plasma fibrin clot permeability coefficient (Ks), turbidimetry and clot lysis time (CLT) were measured in 43 consecutive patients with ET (platelet count from 245 to 991 × 10(3)/µL) and 50 control subjects matched for age, sex and comorbidities. Fibrinolysis proteins and inhibitors together with platelet activation markers were determined. Reduced Ks (-38%, p Ks inversely correlated with fibrinogen, PF4 and C-reactive protein. CLT positively correlated only with PAI-1. Patients with ET display prothrombotic plasma fibrin clot phenotype including impaired fibrinolysis, which represents a new prothrombotic mechanism in this disease.

  9. Multivalent viral capsids with internal cargo for fibrin imaging.

    Directory of Open Access Journals (Sweden)

    Allie C Obermeyer

    Full Text Available Thrombosis is the cause of many cardiovascular syndromes and is a significant contributor to life-threatening diseases, such as myocardial infarction and stroke. Thrombus targeted imaging agents have the capability to provide molecular information about pathological clots, potentially improving detection, risk stratification, and therapy of thrombosis-related diseases. Nanocarriers are a promising platform for the development of molecular imaging agents as they can be modified to have external targeting ligands and internal functional cargo. In this work, we report the synthesis and use of chemically functionalized bacteriophage MS2 capsids as biomolecule-based nanoparticles for fibrin imaging. The capsids were modified using an oxidative coupling reaction, conjugating ∼90 copies of a fibrin targeting peptide to the exterior of each protein shell. The ability of the multivalent, targeted capsids to bind fibrin was first demonstrated by determining the impact on thrombin-mediated clot formation. The modified capsids out-performed the free peptides and were shown to inhibit clot formation at effective concentrations over ten-fold lower than the monomeric peptide alone. The installation of near-infrared fluorophores on the interior surface of the capsids enabled optical detection of binding to fibrin clots. The targeted capsids bound to fibrin, exhibiting higher signal-to-background than control, non-targeted MS2-based nanoagents. The in vitro assessment of the capsids suggests that fibrin-targeted MS2 capsids could be used as delivery agents to thrombi for diagnostic or therapeutic applications.

  10. Artiss Symposium 2014: Psychiatry and Pain Management

    Science.gov (United States)

    2014-01-01

    pain and nociceptive pain . Neuropathic pain may result from direct nerve root injury, radiculopathy, and peripheral de- afferentation pain . This is...the type of pain we see in patients with multi- ple sclerosis, certain strokes, trigeminal neuralgia, and diabetic peripheral neuropathy. Nociceptive ...Gate control theory asserts that activation of nerves that do not transmit pain signals (non- nociceptive fibers) can interfere with signals from pain

  11. Artiss Symposium 2013: Psychiatry and Sleep Disorders

    Science.gov (United States)

    2013-06-05

    greater risk of age-related macular degeneration should also be referred for an ophthalmic evaluation. We often see patients that have post-traumatic...circumstances? We use a more mobile device like a light visor spe- cific with green light at 8,000 to 12,000 lux. The beauty of this device is that it can

  12. Platelet-Rich Fibrin: An Autologous Fibrin Matrix in Surgical Procedures: A Case Report and Review of Literature

    Directory of Open Access Journals (Sweden)

    Amir Hossein Nejat

    2011-01-01

    Full Text Available Introduction: The healing process after surgery is a challenging issue for surgeons. Various materials and techniques have been developed to facilitate this process and reduce its period. Fibrin adhesives are often used in cardiothoracic and vascular surgery to seal diffuse microvascular bleeding and in general and plastic surgery to seal wound borders. This Case report and literature review will introduce the various usages of platelet-rich fibrin in different surgical procedures and the method of producing the matrix. Case Report: A 24-year old man with periorbital skin avulsion treated with PRF membrane has been reported and discussed in this paper.  Conclusion: Platelet-rich fibrin is a natural autologous fibrin matrix, which can be produced with a simple blood sample and a table centrifuge. The material has been used in a wide range of surgical procedures to shorten the healing period and reduce post-surgical complications.

  13. Selective removal of composite sealants with near-UV laser pulses

    Science.gov (United States)

    Louie, Tiffany M.; Jones, Robert S.; Sarma, Anupama V.; Fried, Daniel

    2004-05-01

    It is often necessary to replace pit and fissure sealants and composite restorations. This task is complicated by the necessity for complete removal of the remaining composite to enable suitable adhesion of new composite. Previous studies have shown that lasers pulses from a frequency-tripled Nd:YAG laser (355-nm) can selectively remove residual composite after orthodontic bracket removal on enamel surfaces. UV laser light is preferentially absorbed by polymeric resins and the organic content of the tooth enamel. The objective of this study was to determine if such laser pulses are suitable for selective removal of the old composite from pit and fissure sealants and restorations without damaging surrounding sound tissues. Optical coherence tomography was used to acquire optical cross sections of the occlusal topography and peripheral tooth structure non-destructively before application of the sealants, after sealant application, and after sealant removal with 355-nm laser pulses with intensities ranging from 0-10 J/cm2. Thermocouples were used to monitor the temperature in the pulp chamber during composite removal under clinically relevant ablation rates, i.e., 30 Hz and 30 mJ per laser pulse. At an irradiation intensity of 1.3 J/cm2 pit and fissure sealants were completely removed without visible damage to the underlying enamel. At intensities above 1.5 J/cm2, the laser removes the resin layer while at the same time preferentially etching the surface of the enamel. Temperature excursions in the pulp chamber of extracted teeth was limited to less than 5°C if air-cooling was used during the rapid removal (1-2 min) of sealants, water-cooling was not needed. This is the first presentation of a method for the selective removal of composite restorative materials without damage to the underlying sound tooth structure.

  14. In vitro Microleakage Comparison of Two Fissure Sealants and two Flowable Composite Resins

    Directory of Open Access Journals (Sweden)

    Ahmadi M.

    2012-02-01

    Full Text Available Statement of Problem: Nowadays, fissure sealants are commonly used for sealing the occlusal pit and fissures. However, flowable composites which are more resistance to wear and have a lower amount of shrinkage might be a good substitute for sealants.Purpose: The aim of this study was to compare the amount of micro-leakage between two types of fissure sealants, clinpro and embrace, and two kinds of flowable composites, Filtek flow and Flows-Rite.Materials and Method: 60 intact extracted maxillary permolars were selected and divided into 4 groups. In each group, occlusal fissures of the samples were sealed with fissures sealants (clinpro or Embrace or flowable composites (Filtek flow or Flows-Rite. The apex and furcation of all the teeth were sealed by sealing wax and then the root and crown of the samples were covered by two layers of nail varnish to 1 mm next to the sealant margin. The teeth were immersed in 0.5% fuchsine for 24 hours; then, the teeth were washed and cut off parallel to the linear axis. The microleakage of the samples was studied under the stereomicroscope with a magnification of 16x. Finally, data were analyzed using the Kruskal-Wallis test.Results: There was no significant difference between the amount of microleakage of fissure sealants (clinpro and embrace and flowable composites (filtek flow and Flows-Rite. Conclusion: Under the circumstances of this study and with respect to the clinical situation, it seems that both flowable composite and fissure sealants are suitable materials for pit and fissure sealing.

  15. Fibrin glue mixed with platelet-rich fibrin as a scaffold seeded with dental bud cells for tooth regeneration.

    Science.gov (United States)

    Yang, Kai-Chiang; Wang, Chun-Hao; Chang, Hao-Hueng; Chan, Wing P; Chi, Chau-Hwa; Kuo, Tzong-Fu

    2012-11-01

    Odontogenesis is a complex process with a series of epithelial-mesenchymal interactions and odontogenic molecular cascades. In tissue engineering of teeth from stem cells, platelet-rich fibrin (PRF), which is rich in growth factors and cytokines, may improve regeneration. Accordingly, PRF was added into fibrin glue to enrich the microenvironment with growth factors. Unerupted second molar tooth buds were harvested from miniature swine and cultured in vitro for 3 weeks to obtain dental bud cells (DBCs). Whole blood was collected for the preparation of PRF and fibrin glue before surgery. DBCs were suspended in fibrin glue and then enclosed with PRF, and the DBC-fibrin glue-PRF composite was autografted back into the original alveolar sockets. Radiographic and histological examinations were used to identify the regenerated tooth structure 36 weeks after implantation. Immunohistochemical staining was used to detect proteins specific to tooth regeneration. One pig developed a complete tooth with crown, root, pulp, enamel, dentin, odontoblast, cementum, blood vessels, and periodontal ligaments in indiscriminate shape. Another animal had an unerupted tooth that expressed cytokeratin 14, dentin matrix protein-1, vascular endothelial growth factor, and osteopontin. This study demonstrated, using autogenic cell transplantation in a porcine model, that DBCs seeded into fibrin glue-PRF could regenerate a complete tooth.

  16. Fibronectin alters the rate of formation and structure of the fibrin matrix.

    Science.gov (United States)

    Ramanathan, Anand; Karuri, Nancy

    2014-01-10

    Plasma fibronectin is a vital component of the fibrin clot; however its role on clot structure is not clearly understood. The goal of this study was to examine the influence of fibronectin on the kinetics of formation, structural characteristics and composition of reconstituted fibrin clots or fibrin matrices. Fibrin matrices were formed by adding thrombin to 1, 2 or 4 mg/ml fibrinogen supplemented with 0-0.4 mg/ml fibronectin. The rate of fibrin matrix formation was then monitored by measuring light absorbance properties at different time points. Confocal microscopy of fluorescein conjugated fibrinogen was used to visualize the structural characteristics of fibrin matrices. The amount of fibronectin in fibrin matrices was determined through electrophoresis and immunoblotting of solubilized matrices. Fibronectin concentration positively correlated with the initial rate of fibrin matrix formation and with steady state light absorbance values of fibrin matrices. An increase in fibronectin concentration resulted in thinner and denser fibers in the fibrin matrices. Electrophoresis and immunoblotting showed that fibronectin was covalently and non-covalently bound to fibrin matrices and in the form of high molecular weight multimers. The formation of fibronectin multimers was attributed to cross-linking of fibronectin by trace amounts Factor XIIIa. These findings are novel because they link results from light absorbance studies to microcopy analyses and demonstrate an influence of fibronectin on fibrin matrix structural characteristics. This data is important in developing therapies that destabilize fibrin clots.

  17. A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo

    Science.gov (United States)

    Motley, Michael P.; Madsen, Daniel H.; Jürgensen, Henrik J.; Spencer, David E.; Szabo, Roman; Holmbeck, Kenn; Flick, Matthew J.; Lawrence, Daniel A.; Castellino, Francis J.; Weigert, Roberto

    2016-01-01

    Extravascular fibrin deposition accompanies many human diseases and causes chronic inflammation and organ damage, unless removed in a timely manner. Here, we used intravital microscopy to investigate how fibrin is removed from extravascular space. Fibrin placed into the dermis of mice underwent cellular endocytosis and lysosomal targeting, revealing a novel intracellular pathway for extravascular fibrin degradation. A C-C chemokine receptor type 2 (CCR2)-positive macrophage subpopulation constituted the majority of fibrin-uptaking cells. Consequently, cellular fibrin uptake was diminished by elimination of CCR2-expressing cells. The CCR2-positive macrophage subtype was different from collagen-internalizing M2-like macrophages. Cellular fibrin uptake was strictly dependent on plasminogen and plasminogen activator. Surprisingly, however, fibrin endocytosis was unimpeded by the absence of the fibrin(ogen) receptors, αMβ2 and ICAM-1, the myeloid cell integrin-binding site on fibrin or the endocytic collagen receptor, the mannose receptor. The study identifies a novel fibrin endocytic pathway engaged in extravascular fibrin clearance and shows that interstitial fibrin and collagen are cleared by different subsets of macrophages employing distinct molecular pathways. PMID:26647393

  18. Clinical and SEM assessment of ART high-viscosity glass-ionomer sealants after 8-13 years in 4 teeth.

    NARCIS (Netherlands)

    Frencken, J.E.F.M.; Wolke, J.G.C.

    2010-01-01

    OBJECTIVES: Resin composite sealants are retained longer than low-viscosity glass-ionomer sealants. Nevertheless, a systematic review showed that there is no evidence that resin composite sealants are superior to low-viscosity glass-ionomers in preventing dentine carious lesion development. This cas

  19. PAH Concentrations Decline Following 2006 Ban on Coal-Tar-Based Pavement Sealants in Austin, Texas

    Science.gov (United States)

    Van Metre, P. C.; Mahler, B. J.

    2013-12-01

    Recent studies have concluded that coal-tar-based pavement sealants (CT sealants) are a major source of polycyclic aromatic hydrocarbons (PAHs) in non-industrial urban settings in the United States. In 2006, Austin, TX, became the first jurisdiction in the U.S. to ban the use of CT sealants. We evaluated PAH concentrations following the ban by analyzing sediment cores collected from Lady Bird Lake in 2012; Lady Bird Lake impounds the Colorado River in central Austin and receives runoff from much of the greater Austin area. The mean sum concentration of the 16 U.S. Environmental Protection Agency Priority Pollutant PAHs (∑PAH16) in one of two 2012 sediment cores analyzed for PAHs declined 75% from before 2006 (mean of 4 samples=8,090 μg kg-1) to 2012 (mean of 2 samples=2,030 μg kg-1), reversing a 40-year (1959-1999) upward trend in PAH concentrations that was previously documented. The downward trend in PAH concentrations in the seven uppermost 1 cm sampling intervals in the first 2012 core was statistically significant (r=0.93, p-value=0.002). Post-2008 PAH trends in the second 2012 core were similar (significant downward trend in the six uppermost 1 cm sampling intervals and mean 2012 ∑PAH16 of 2,390 μg kg-1); however, pre-2007 sediment did not appear to have been preserved in this core likely because of the effects of flooding on sediment deposition and mixing at this site--the largest flood on the Colorado River in Austin in 20 years was in 2007. On the basis of a comparison of lake-sediment PAH profiles to 22 PAH source profiles, the PAH loading to lake sediment continues to be dominated by CT sealants. The continued dominance of proportional PAH loading by CT sealants in spite of decreased concentrations since 2006 might be because legacy CT sealant and contaminated soils and sediments continue to yield PAHs to runoff. A previous study using source-receptor modeling concluded that CT sealants were the largest PAH source to 40 urban lakes studied in the

  20. Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization.

    Science.gov (United States)

    Lugovskoy, Eduard V; Gritsenko, Pavel G; Koshel, Tatyana A; Koliesnik, Ievgen O; Cherenok, Serhey O; Kalchenko, Olga I; Kalchenko, Vitaliy I; Komisarenko, Serhey V

    2011-04-01

    Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with respect to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid (C-192), in which case the maximum rate of fibrin polymerization in the fibrinogen + thrombin reaction decreased by 50% at concentrations of 0.52 × 10(-6) M (IC(50)). At this concentration, the molar ratio of the compound to fibrinogen was 1.7 : 1. For the case of desAABB fibrin polymerization, the IC(50) was 1.26 × 10(-6) M at a molar ratio of C-192 to fibrin monomer of 4 : 1. Dipropoxycalix[4]arene bis-methylene-bis-phosphonic acid (C-98) inhibited fibrin desAABB polymerization with an IC(50) = 1.31 × 10(-4) M. We hypothesized that C-192 blocks fibrin formation by combining with polymerization site 'A' (Aα17-19), which ordinarily initiates protofibril formation in a 'knob-hole' manner. This suggestion was confirmed by an HPLC assay, which showed a host-guest inclusion complex of C-192 with the synthetic peptide Gly-Pro-Arg-Pro, an analogue of site 'A'. Further confirmation that the inhibitor was acting at the initial step of the reaction was obtained by electron microscopy, with no evidence of protofibril formation being evident. Calixarene C-192 also doubled both the prothrombin time and the activated partial thromboplastin time in normal human blood plasma at concentrations of 7.13 × 10(-5) M and 1.10 × 10(-5) M, respectively. These experiments demonstrate that C-192 is a specific inhibitor of fibrin polymerization and blood coagulation and can be used for the design of a new class of antithrombotic agents.

  1. Ultrastructural changes to rabbit fibrin and platelets due to aspartame.

    Science.gov (United States)

    Pretorius, E; Humphries, P

    2007-01-01

    The coagulation process, including thrombin, fibrin, as well as platelets, plays an important role in hemostasis, contributing to the general well-being of humans. Fibrin formation and platelet activation are delicate processes that are under the control of many small physiological events. Any one of these many processes may be influenced or changed by external factors, including pharmaceutical or nutritional products, e.g., the sweetener aspartame (L-aspartyl-L-phenylalanine methyl ester). It is known that phenylalanine is present at position P(9) and aspartate at position P(10) of the alpha-chain of human fibrinogen, and plays an important role in the conversion of fibrinogen to fibrin by the catalyst alpha-thrombin. The authors investigate the effect of aspartame on platelet and fibrin ultrastructure, by using the rabbit animal model and the scanning electron microscope. Animals were exposed to 34 mg/kg of aspartame 26x during a 2-month period. Aspartame-exposed fibrin networks appeared denser, with a thick matted fine fiber network covering thick major fibers. Also, the platelet aggregates appeared more granular than the globular control platelet aggregates. The authors conclude by suggesting that aspartame usage may interfere with the coagulation process and might cause delayed fibrin breakup after clot formation. They suggest this, as the fibrin networks from aspartame-exposed rabbits are more complex and dense, due to the netlike appearance of the minor, thin fibers. Aspartame usage should possibly be limited by people on anti-clotting medicine or those with prone to clot formation.

  2. Development of a Teat Bio-sealant and Evaluation of its Technological and Functional Properties.

    Science.gov (United States)

    Serna-Cock, Liliana; Pabón-Rodríguez, Omar Vladimir

    2016-06-01

    A teat bio-sealant was developed using Weissella cibaria, and the bio-sealant's technological and functional properties were assessed. The development included four experimental phases that were analyzed using independent experimental designs. Initially, sterilized or pasteurized Aloe vera gels were used, and the effect of heat treatment was investigated. In the second phase, the effects of time, storage temperature, and addition of cryopreservatives on the viability of the probiotic were observed. The third phase consisted of evaluating the synergistic effects of the cryopreservatives. The fourth phase involved selecting a material that would provide viscosity to the teat sealant. Technological and functional properties were measured in terms of viability of W. cibaria, and antimicrobial activity against Staphylococcus aureus and Streptococcus agalactiae was also analyzed. A mixture of milk powder and glycerol preserved this antimicrobial activity. Pullulan provided greater viscosity and maintained the technological and functional properties of the bio-sealant for 29 days. This teat bio-sealant can be used as an alternative for the prevention of bovine mastitis.

  3. Impact of adhesive and photoactivation method on sealant integrity and polymer network formation

    Directory of Open Access Journals (Sweden)

    Boniek Castillo Dutra Borges

    2012-06-01

    Full Text Available We evaluated the influence of photoactivation method and hydrophobic resin (HR application on the marginal and internal adaptation, hardness (KHN, and crosslink density (CLD of a resin-based fissure sealant. Model fissures were created in bovine enamel fragments (n = 10 and sealed using one of the following protocols: no adhesive system + photoactivation of the sealant using continuous light (CL, no adhesive system + photoactivation of the sealant using the soft-start method (SS, HR + CL, or HR + SS. Marginal and internal gaps and KHN were assessed after storage in water for 24 h. The CLD was indirectly assessed by repeating the KHN measurement after 24 h of immersion in 100% ethanol. There was no difference among the samples with regard to marginal or internal adaptation. The KHN and CLD were similar for samples cured using either photoactivation method. Use of a hydrophobic resin prior to placement of fissure sealants and curing the sealant using the soft-start method may not provide any positive influence on integrity or crosslink density.

  4. Evaluation on High-temperature Adhesion Performance of Hot-applied Sealant for Asphalt Pavement

    Institute of Scientific and Technical Information of China (English)

    LI Feng; LI Tinggang; SHI Xiaopei

    2014-01-01

    In order to investigate the high-temperature performances of the asphalt pavement hot-applied sealant, as well as to reduce failures of the sealant pullout, the softening point test and the flow test (two existing methods for evaluating high-temperature performances) were conducted. It was found that both tests could not accurately reflect the adhesion performances of the sealant at high temperatures. For this purpose, the adhesion test for PSAT (pressure sensitive adhesive tape) has been taken as a reference to develop a device that is suitable for evaluating the adhesion performances, by modifying relevant test parameters according to the road conditions at high temperatures. Thirteen common sealants were tested in the modified adhesion test, softening point test and flow test. The experimental results show that no significant correlation (p>0.05) exists between the adhesion value, softening point, adhesion value and flow value;while a significant correlation (p<0.05) exists between the softening point and flow value. The modified adhesion test is efficient in distinguishing the high-temperature adhesion performances of different sealants, and can be used as a standard method for evaluating such performances.

  5. Polychlorinated biphenyls (PCBs) in indoor air originating from sealants in contaminated and uncontaminated apartments within the same housing estate

    DEFF Research Database (Denmark)

    Frederiksen, Marie; Meyer, Harald William; Ebbehøj, Niels Erik;

    2012-01-01

    Twenty-four congeners of polychlorinated biphenyls (PCBs) were measured in 83 air samples and 20 elastic sealants samples of apartments with PCB-containing sealants. In addition, PCBs were measured in 21 air samples from reference apartments located in an uncontaminated section of the same estate...

  6. Platelet rich fibrin in jaw defects

    Science.gov (United States)

    Nica, Diana; Ianes, Emilia; Pricop, Marius

    2016-03-01

    Platelet rich fibrin (PRF) is a tissue product of autologous origin abundant in growth factors, widely used in regenerative procedures. Aim of the study: Evaluation of the regenerative effect of PRF added in the bony defects (after tooth removal or after cystectomy) Material and methods: The comparative nonrandomized study included 22 patients divided into 2 groups. The first group (the test group) included 10 patients where the bony defects were treated without any harvesting material. The second group included 12 patients where the bony defects were filled with PRF. The bony defect design was not critical, with one to two walls missing. After the surgeries, a close clinically monitoring was carried out. The selected cases were investigated using both cone beam computer tomography (CBCT) and radiographic techniques after 10 weeks postoperatively. Results: Faster bone regeneration was observed in the bony defects filled with PRF comparing with the not grafted bony defects. Conclusions: PRF added in the bony defects accelerates the bone regeneration. This simplifies the surgical procedures and decreases the economic costs.

  7. Identification of fibrin clot-bound plasma proteins.

    Directory of Open Access Journals (Sweden)

    Simone Talens

    Full Text Available Several proteins are known to bind to a fibrin network and to change clot properties or function. In this study we aimed to get an overview of fibrin clot-bound plasma proteins. A plasma clot was formed by adding thrombin, CaCl(2 and aprotinin to citrated platelet-poor plasma and unbound proteins were washed away with Tris-buffered saline. Non-covalently bound proteins were extracted, separated with 2D gel electrophoresis and visualized with Sypro Ruby. Excised protein spots were analyzed with mass spectrometry. The identity of the proteins was verified by checking the mass of the protein, and, if necessary, by Western blot analysis. Next to established fibrin-binding proteins we identified several novel fibrin clot-bound plasma proteins, including α(2-macroglobulin, carboxypeptidase N, α(1-antitrypsin, haptoglobin, serum amyloid P, and the apolipoproteins A-I, E, J, and A-IV. The latter six proteins are associated with high-density lipoprotein particles. In addition we showed that high-density lipoprotein associated proteins were also present in fibrinogen preparations purified from plasma. Most plasma proteins in a fibrin clot can be classified into three groups according to either blood coagulation, protease inhibition or high-density lipoprotein metabolism. The presence of high-density lipoprotein in clots might point to a role in hemostasis.

  8. Measurement of clottability of fibrin polymers using magnetic orientation

    Science.gov (United States)

    Iwasaka, M.; Ueno, S.; Tsuda, H.

    1996-04-01

    Fibrin polymers, as a kind of diamagnetic material, are oriented parallel to the direction of magnetic fields. We investigated the polymerization of partially digested fibrinogens in an 8 T magnetic field in order to observe the clotting ability of various sizes of fibrinogen fractions using a magnetic orientation technique. We purified high-molecular weight fraction (F1) and low-molecular weight fraction from human fibrinogen (F2). Fibrin gels were formed in an 8 T magnetic field for 9 h, and transmittancies were measured to evaluate the degree of magnetic orientation. The results show that a lack at the C-terminal half of one Aα chain did not affect the magnetic orientation of fibrin. We also investigated the effect of the digestion of fibrinogen by plasmin on the magnetic orientation of fibrin. The result shows that partially digested fibrin molecules also orient in an 8 T magnetic field. However, the degree of magnetic orientation significantly decreases when fragment X and fragment Y appear.

  9. Phase-separated chitosan-fibrin microbeads for cell delivery.

    Science.gov (United States)

    Chen, Zhewei; Wang, Limin; Stegemann, Jan P

    2011-01-01

    Matrix-enhanced delivery of cells is a promising approach to improving current cell therapies. Our objective was to create cell-laden composite microbeads that combine the attractive features of the natural polymers chitosan and fibrin. Liquid polydimethylsiloxane was used to emulsify a chitosan-fibrinogen solution containing suspended human fibroblast cells, followed by initiation of thrombin-mediated polymerization of fibrin and thermal/pH-mediated gelation of chitosan. Chitosan/fibrin weight percent (wt%) ratios of 100/0, 75/25, 50/50 and 25/75 were investigated. Microbead diameters ranged from 275 ± 99 µm to 38 ± 10 µm using impeller speeds from 600 to 1400 rpm. Fibroblasts remained viable on day 1 post-fabrication in all matrices, but cell viability was markedly higher in high-fibrin microbeads by day 8 post-fabrication. Cell spreading and interaction with the extracellular matrix was also markedly increased in high-fibrin matrices. Such composite microbeads containing viable entrapped cells have potential for minimally invasive delivery of cells for a variety of tissue repair applications.

  10. Alignment of the Fibrin Network Within an Autologous Plasma Clot.

    Science.gov (United States)

    Gessmann, Jan; Seybold, Dominik; Peter, Elvira; Schildhauer, Thomas Armin; Köller, Manfred

    2016-01-01

    Autologous plasma clots with longitudinally aligned fibrin fibers could serve as a scaffold for longitudinal axonal regrowth in cases of traumatic peripheral nerve injuries. Three different techniques for assembling longitudinally oriented fibrin fibers during the fibrin polymerization process were investigated as follows: fiber alignment was induced by the application of either a magnetic field or-as a novel approach-electric field or by the induction of orientated flow. Fiber alignment was characterized by scanning electron microscopy analysis followed by image processing using fast Fourier transformation (FFT). Besides FFT output images, area xmin to xmax, as well as full width at half maximum (FWHM) of the FFT graph plot peaks, was calculated to determine the relative degree of fiber alignment. In addition, fluorescently labeled human fibrinogen and mesenchymal stem cells (MSCs) were used to visualize fibrin and cell orientation in aligned and nonaligned plasma clots. Varying degrees of fiber alignment were achieved by the three different methods, with the electric field application producing the highest degree of fiber alignment. The embedded MSCs showed a longitudinal orientation in the electric field-aligned plasma clots. The key feature of this study is the ability to produce autologous plasma clots with aligned fibrin fibers using physical techniques. This orientated internal structure of an autologous biomaterial is promising for distinct therapeutic applications, such as a guiding structure for cell migration and growth dynamics.

  11. Weight reduction is associated with increased plasma fibrin clot lysis.

    Science.gov (United States)

    Brzezińska-Kolarz, Beata; Kolarz, Marek; Wałach, Angelika; Undas, Anetta

    2014-11-01

    Obesity is associated with an increased risk of vascular thrombotic events. We sought to investigate how obesity and weight loss affect plasma fibrin clot properties. A total of 29 obese patients were studied before and after 3-month low-fat diet. Plasma fibrin clot parameters, including fibrin clot permeation coefficient (Ks), the lag phase of the turbidity curve, clot lysis time (t 50%), maximum rate of increase in D-dimer levels, and maximum D-dimer concentrations, were determined. Low-fat diet resulted in the reduction of body weight (P < .0001), body mass index (P < .0001), fat mass (P < .0001), total cholesterol (P < .0001), low-density lipoprotein cholesterol (P = .0005), triglycerides (P = .008), and plasminogen activator inhibitor 1 (P = .02), but not in fibrinogen or C-reactive protein. The only change in fibrin clot variables was shorter t 50% (P = .02). Baseline t 50%, but not posttreatment, correlated with waist circumference (r = .44, p = .02). This study demonstrates that weight loss in obese people can increase the efficiency of fibrin clot lysis.

  12. Effect of collagen sponge and fibrin glue on bone repair

    Directory of Open Access Journals (Sweden)

    Thiago de Santana SANTOS

    2015-12-01

    Full Text Available ABSTRACT The ability of hemostatic agents to promote bone repair has been investigated using in vitro and in vivo models but, up to now, the results are inconclusive. Objective In this context, the aim of this study was to compare the potential of bone repair of collagen sponge with fibrin glue in a rat calvarial defect model. Material and Methods Defects of 5 mm in diameter were created in rat calvariae and treated with either collagen sponge or fibrin glue; untreated defects were used as control. At 4 and 8 weeks, histological analysis and micro-CT-based histomorphometry were carried out and data were compared by two-way ANOVA followed by Student-Newman-Keuls test when appropriated (p≤0.05. Results Three-dimensional reconstructions showed increased bone formation in defects treated with either collagen sponge or fibrin glue compared with untreated defects, which was confirmed by the histological analysis. Morphometric parameters indicated the progression of bone formation from 4 to 8 weeks. Additionally, fibrin glue displayed slightly higher bone formation rate when compared with collagen sponge. Conclusion Our results have shown the benefits of using collagen sponge and fibrin glue to promote new bone formation in rat calvarial bone defects, the latter being discreetly more advantageous.

  13. Modification of silicone sealant to improve gamma radiation resistance, by addition of protective agents

    Science.gov (United States)

    González-Pérez, Giovanni; Burillo, Guillermina

    2013-09-01

    Poly (dimethylsiloxane) (PDMS) sealant (SS) was modified with the addition of different protective compounds to conserve its physical-chemical properties during gamma irradiation. 2-Vinyl naphthalene (2-VN), bisphenol-A (BPA) and poly (vinyl carbazole) (PVK) were used to evaluate radiation protection through the crosslinking effect of radiation. The samples were irradiated with doses from 100 kGy to 500 kGy at room temperature in air, with a 60Co gamma source, and the changes in molecular weight, thermal behavior, elastic properties and infrared spectra (FTIR-ATR) absorbance analysis were determined. The molecular weight of unmodified silicone sealant increases with the absorbed dose because of crosslinking as predominant effect. However, the crosslinking effect was inhibited with the addition of protective agent due to the aromatic compounds present. Modified silicone sealant films present better radiation resistance than unmodified system.

  14. Glass-ceramic sealant for solid oxide fuel cells application: Characterization and performance in dual atmosphere

    Science.gov (United States)

    Sabato, A. G.; Cempura, G.; Montinaro, D.; Chrysanthou, A.; Salvo, M.; Bernardo, E.; Secco, M.; Smeacetto, F.

    2016-10-01

    A glass-ceramic composition was designed and tested for use as a sealant in solid oxide fuel cell (SOFC) planar stack design. The crystallization behaviour was investigated by calculating the Avrami parameter (n) and the activation energy for crystallization (Ec) was obtained. The calculated values for n and Ec were 3 and 413.5 kJ/mol respectively. The results of thermal analyses indicate that this composition shows no overlap between the sintering and crystallization stages and thus an almost pore-free sealant can be deposited and sintered at 850 °C in air for 30 min. A gas tightness test has been carried out at 800 °C for 1100 h in dual atmosphere (Ar-H2 and air) without recording any leakage. Morphological and crystalline phase analyses were conducted prior and following tests in dual atmospheres in order to assess the compatibility of the proposed sealant with the metallic interconnect.

  15. Histological effects of fibrin glue on nervous tissue: a safety study in rats.

    NARCIS (Netherlands)

    Vries, J. de; Menovsky, T.; Gulik, S. van; Wesseling, P.

    2002-01-01

    BACKGROUND: Little is known of the histologic effects of fibrin glue on normal nervous tissue. To verify the safety of intracranial application of fibrin glue, we investigated the histologic effects of fibrin glue on brain tissue and intracranial nerves of rats. METHODS: In Group I (n =12), bifronta

  16. 21 CFR 864.7320 - Fibrinogen/fibrin degradation products assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Fibrinogen/fibrin degradation products assay. 864....7320 Fibrinogen/fibrin degradation products assay. (a) Identification. A fibrinogen/fibrin degradation products assay is a device used to detect and measure fibrinogen degradation products and...

  17. A CCR2 macrophage endocytic pathway mediates extravascular fibrin clearance in vivo

    DEFF Research Database (Denmark)

    Motley, Michael P; Madsen, Daniel H; Jürgensen, Henrik J;

    2016-01-01

    Extravascular fibrin deposition accompanies many human diseases and causes chronic inflammation and organ damage, unless removed in a timely manner. Here, we used intravital microscopy to investigate how fibrin is removed from extravascular space. Fibrin placed into the dermis of mice underwent c...

  18. Microleakage, adaptation ability and clinical efficacy of two fluoride releasing fissure sealants

    Directory of Open Access Journals (Sweden)

    Marković Dejan

    2012-01-01

    Full Text Available Background/Aim. Retention of fissure sealants and good adaptation to enamel are essential for their success. Fluoride releasing resin-based materials are widely accepted for pit and fissure sealing, but newly designed glass ionomers can serve as a good alternative. The aim of this study was to evaluate microleakage and sealing ability in vitro, and to clinically assess two fluoride releasing fissure sealants. Methods. The sample for experimental study consisted of 20 freshly extracted intact human third molars, divided in two experimental groups according to the sealing material: fluoride releasing resin-based (Heliosel F and glass ionomer (Fuji Triage material. Digital images and scanning electron microscope were used to assess microleakage and adaptation ability. Sample for clinical study consisted of 60 children, aged 6-8 years, with high caries risk, divided in two groups according to the sealant material. Fissure sealant was applied to all erupted, caries-free first permanent molars. Sealants were evaluated after 3, 6 and 12 months using modified Ryge criteria for retention, marginal adaptation, colour match, surface smoothness and caries. Results. Microleakage was detected in more than half of the specimen, without significant differences between the two groups (p > 0.05. Both materials exhibited acceptable sealing ability. Complete retention at the end of the observation period was 81.8% for resin-based, and 21.1% for glass-ionomer fissure sealant (p < 0.001. The presence of caries in sealed molars has been detected in one patient in both groups. During the 12-month observation period, Helioseal F demonstrated better retention, marginal adaptation and surface smoothness (p < 0.001. There were no differences between the two materials regarding caries and color match (p > 0.05. Conclusion. Both tested materials demonstrate satisfactory clinical and caries prophylactic characteristics that justify their use in contemporary preventive

  19. Characterisation and microleakage of a new hydrophilic fissure sealant - UltraSeal XT® hydro™

    Science.gov (United States)

    GÜÇLÜ, Zeynep A.; DÖNMEZ, Nazmiye; HURT, Andrew P.; COLEMAN, Nichola J.

    2016-01-01

    ABSTRACT Objectives The aim of this study was to characterise the new hydrophilic fissure sealant, UltraSeal XT® hydro™ (Ultradent Products, USA), and to investigate its in vitro resistance to microleakage after placement on conventionally acid etched and sequentially lased and acid etched molars. Material and Methods The sealant was characterised by Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and Vickers indentation test. Occlusal surfaces of extracted human molars were either conventionally acid etched (n=10), or sequentially acid etched and laser irradiated (n=10). UltraSeal XT® hydro™ was applied to both groups of teeth which were then subjected to 2,500 thermocycles between 5 and 55°C prior to microleakage assessment by fuchsin dye penetration. Results UltraSeal XT® hydro™ is an acrylate-based sealant that achieved a degree of conversion of 50.6±2.2% and a Vickers microhardness of 24.2±1.5 under standard light curing (1,000 mWcm-2 for 20 s). Fluoride ion release is negligible within a 14-day period. SEM and EDX analyses indicated that the sealant comprises irregular submicron and nano-sized silicon-, barium-, and aluminium-bearing filler phases embedded in a ductile matrix. Laser preconditioning was found to significantly reduce microleakage (Mann-Whitney U test, proughness on a 50 to 100 μm scale that caused the segregation and concentration of the filler particles at the enamel-sealant interface. Conclusion Laser preconditioning significantly decreased microleakage and increased enamel surface roughness, which caused zoning of the filler particles at the enamel-sealant interface. PMID:27556205

  20. Anti Streptococcus mutans non fluoride and fluoride containing sealants after adding nano-silver particles

    Directory of Open Access Journals (Sweden)

    Maryam Ghasempour

    2014-04-01

    Full Text Available   Background and Aims: Since recurrent caries are one of the major causes of failure in resin restorations, the production of antibacterial resin composites was always under investigation. The aim of this study was to evaluate the efficacy of fissure sealants containing nanosilver particles against the Streptococcus mutans.   Materials and Methods: In this experimental study, the antibacterial properties of two sealants (with fluoride (Clinpro 3M and without fluoride (Concise 3M was investigated with 0, 0.01, 0.02, 0.03, 0.04, 0.05% w/w after adding nano-silver using direct contact test. Sealants formed on the walls of 500ml micro tube and after curing, they left in contact with bacterial suspension. In periods of 3, 24, 48h, a 10 µl volume of liquid medium was placed in blood agar culture and after 24 h incubation at 37ºC, the number of S.mutans colony was counted by colony counter. Data were analyzed using ANOVA andT-test.   Results: Results reported sealants with fluoride comparing to non fluoride ones had significant effect on inhibition of S.mutans growth (P<0.001. The direct contact test demonstrated that by increasing the amount of nano particles, the bacterial growth was significantly diminished (P<0.001.   Conclusion: While sealants with fluoride demonstrated antibacterial effect, sealants with incorporation of higher weight percentage of nanosilver particles, had stronger and more significant antibacterial effect in direct contact test.

  1. Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial.

    Science.gov (United States)

    Madurantakam, Parthasarathy; Yoganarasimha, Suyog; Hasan, Fadi K

    2015-09-29

    Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral surgery. Unlike platelet rich plasma (PRP) that is a gel or a suspension, Leukocyte-Platelet Rich Fibrin (L-PRF) is a solid 3D fibrin membrane generated chair-side from whole blood containing no anti-coagulant. The membrane has a dense three dimensional fibrin matrix with enriched platelets and abundant growth factors. L-PRF is a popular adjunct in surgeries because of its superior handling characteristics as well as its suturability to the wound bed. The goal of the study is to demonstrate generation as well as provide detailed characterization of relevant properties of L-PRF that underlie its clinical success.

  2. Enamel Surface with Pit and Fissure Sealant Containing 45S5 Bioactive Glass.

    Science.gov (United States)

    Yang, S-Y; Kwon, J-S; Kim, K-N; Kim, K-M

    2016-05-01

    Enamel demineralization adjacent to pit and fissure sealants leads to the formation of marginal caries, which can necessitate the replacement of existing sealants. Dental materials with bioactive glass, which releases ions that inhibit dental caries, have been studied. The purpose of this study was to evaluate the enamel surface adjacent to sealants containing 45S5 bioactive glass (BAG) under simulated microleakage between the material and the tooth in a cariogenic environment. Sealants containing 45S5BAG filler were prepared as follows: 0% 45S5BAG + 50.0% glass (BAG0 group), 12.5% 45S5BAG + 37.5% glass (BAG12.5 group), 25.0% 45S5BAG + 25.0% glass (BAG25.0 group), 37.5% 45S5BAG + 12.5% glass (BAG37.5 group), and 50.0% 45S5BAG + 0% glass (BAG50.0 group). A cured sealant disk was placed over a flat bovine enamel disk, separated by a 60-µm gap, and immersed in lactic acid solution (pH 4.0) at 37 °C for 15, 30, and 45 d. After the storage period, each enamel disk was separated from the cured sealant disk, and the enamel surface was examined with optical 3-dimensional surface profilometer, microhardness tester, and scanning electron microscopy. The results showed a significant increase in roughness and a decrease in microhardness of the enamel surface as the proportion of 45S5BAG decreased (Penamel surfaces with BAG50.0 showed a smooth surface, similar to those in the control group with distilled water, even after prolonged acid storage. Additionally, an etched pattern was observed on the surface of the demineralized enamel with a decreasing proportion of 45S5BAG. Increasing the 45S5BAG filler contents of the sealants had a significant impact in preventing the demineralization of the enamel surface within microgaps between the material and the tooth when exposed to a cariogenic environment. Therefore, despite some marginal leakage, these novel sealants may be effective preventive dental materials for inhibiting secondary caries at the margins.

  3. Estimating emissions from adhesives and sealants uses and manufacturing for environmental risk assessments.

    Science.gov (United States)

    Tolls, Johannes; Gómez, Divina; Guhl, Walter; Funk, Torsten; Seger, Erich; Wind, Thorsten

    2016-01-01

    Regulation (EC) No 1907/2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) requires that environmental exposure assessments be performed for all uses of dangerous substances that are marketed in the European Union in quantities above 10 tons per year. The quantification of emissions to the environment is a key step in this process. This publication describes the derivation of release factors and gives guidance for estimating use rates for quantifying the emissions from the manufacturing and application of adhesives and sealants. Release factors available for coatings and paints are read across to adhesives or sealants based on similarities between these 2 product groups with regard to chemical composition and to processing during manufacturing and application. The granular emission scenarios in these documents are mapped to the broad emission scenarios for adhesives or sealants. According to the mapping, the worst-case release factors for coatings or paints are identified and assigned to the adhesives or sealants scenarios. The resulting 10 specific environmental release categories (SPERCs) for adhesives and sealants are defined by differentiating between solvent and nonsolvent ingredients and between water-borne and solvent-borne or solvent-free products. These cover the vast majority of the production processes and uses and are more realistic than the 5 relevant emission estimation defaults provided in the REACH guidance. They are accompanied with adhesive or sealant consumption rates in the EU and with guidance for estimating conservative substance use rates at a generic level. The approach of combining conservative SPERC release factors with conservative estimates of substance rates is likely to yield emission estimates that tend to overpredict actual releases. Because this qualifies the approach for use in lower-tier environmental exposure assessment, the Association of the European Adhesive & Sealant Industry

  4. Fibrin Adhesive: Clinical Application in Coronary Artery Bypass Graft Surgery

    Science.gov (United States)

    Fundaró, Pino; Velardi, Antonio R.; Santoli, Carmine

    1985-01-01

    Fibrin adhesive was used 72 times in a group of 67 patients undergoing elective coronary artery bypass graft surgery. The indications were prophylactic sealing of potential sources of bleeding, topical hemostasis (control of bleeding sites dangerous or difficult to suture), and fixation of the graft in the optimal position. The method of glue application under varying circumstances is described and the results are reported. This experience suggests that in some cases the glue expedites the operation and makes it safer. We conclude that the fibrin sealing represents a valid aid in coronary artery bypass graft surgery. PMID:15227018

  5. Caries-preventive Effect of Supervised Toothbrushing and Sealants.

    Science.gov (United States)

    Hilgert, L A; Leal, S C; Mulder, J; Creugers, N H J; Frencken, J E

    2015-09-01

    To investigate the effectiveness of 3 caries-preventive measures on high- and low-caries risk occlusal surfaces of first permanent molars over 3 y. This cluster-randomized controlled clinical trial covered 242 schoolchildren, 6 to 7 y old, from low socioeconomic areas. At baseline, caries risk was assessed at the tooth surface level, through a combination of ICDAS II (International Caries Detection and Assessment System) and fissure depth codes. High-caries risk occlusal surfaces were treated according to daily supervised toothbrushing (STB) at school and 2 sealants: composite resin (CR) and atraumatic restorative treatment-high-viscosity glass-ionomer cement (ART-GIC). Low-caries risk occlusal surfaces received STB or no intervention. Evaluations were performed after 0.5, 1, 2, and 3 y. A cavitated dentine carious lesion was considered a failure. Data were analyzed according to the proportional hazard rate regression model with frailty correction, Wald test, analysis of variance, and t test, according to the jackknife procedure for calculating standard errors. The cumulative survival rates of cavitated dentine carious lesion-free, high-caries risk occlusal surfaces were 95.6%, 91.4%, and 90.2% for STB, CR, and ART-GIC, respectively, over 3 y, which were not statistically significantly different. For low-caries risk occlusal surfaces, no statistically significant difference was observed between the cumulative survival rate of the STB group (94.8%) and the no-intervention group (92.1%) over 3 y. There was neither a difference among STB, CR, and ART-GIC on school premises in preventing cavitated dentine carious lesions in high-caries risk occlusal surfaces of first permanent molars nor a difference between STB and no intervention for low-caries risk occlusal surfaces of first permanent molars over 3 y.

  6. The Effectiveness of Fissure Sealant Therapy Placed by Professional Complementary to Dentistry Compared with Dentists

    Directory of Open Access Journals (Sweden)

    F Nilchian

    2005-10-01

    Full Text Available Background: Dental caries is a process that may take place on any tooth surface in the oral cavity where a microbial biofilm (dental plaque is allowed to develop over a period. From the public health perspective, the prevention of caries is still a major challenge. The development of dental caries within the mouth follows a fixed hierarchy indicating that tooth surfaces vary in caries susceptibility. The most susceptible surfaces are the buccal pits and occlusal-fissured surfaces of the first molar teeth. Since the 1960`s many trials have investigated the effectiveness of using sealants on pit and fissure surfaces. However, the cost effectiveness of sealants is an important issue considered by many studies noted that cost-effectiveness of sealants would be enhanced by using trained auxiliaries to apply them. The changes in dentistry and oral health reveal a need to review the roles of dental auxiliaries in order to deliver quality care cost-effectively. There were number of studies conducted on the effectiveness of dental auxiliaries around the world. According to the purpose of this project, studies evaluated the effectiveness of Professional complementary to dentistry (PCDs and different type of dental auxiliaries in carrying out complete restorations and in the preventive therapies, fissure sealants, traumatic restorative treatment were evaluated in literature review. The aim of the present study is to review the literature and assess whether PCDs can perform pit and fissure sealants as effectively as dentist through investigation of the caries preventive effect of sealant placed by dentist relative to sealant placed by PCDs. Method: Electronic databases were searched till January 2005. The databases which were used are: Medline via Ovid, Cochrane databases of systematic review , DARE (Database of Abstracts of Reviews of Effectiveness, CCTR (Clinical Controlled Trials Register Cochrane Library, Dissertation Abstracts International database

  7. Discrimination between Fibrin and Fibrinogen by a Monoclonal Antibody against a Synthetic Peptide

    Science.gov (United States)

    Scheefers-Borchel, Ursula; Muller-Berghaus, Gert; Fuhge, Peter; Eberle, Reinhard; Heimburger, Nobert

    1985-10-01

    Circulating soluble fibrin, observed in the blood of patients with ongoing intravascular coagulation, is generated from the plasma protein fibrinogen by the limited proteolytic action of thrombin. We report the production of a monoclonal antibody that discriminates between fibrin and fibrinogen in blood. The synthetic hexapeptide Gly-Pro-Arg-Val-Val-Glu, representing the amino terminus of the α chain of human fibrin, was used as immunogen. This hexapeptide is located within the Aα chain of fibrinogen but becomes the amino terminus of the fibrin α chain, after fibrinopeptide A is removed by the action of thrombin, and thus becomes accessible for antibody binding. The monoclonal antibody we have prepared can discriminate between fibrin and fibrinogen and thus can be used in assay systems to quantitate soluble fibrin or, potentially, to image fibrin-rich thrombi.

  8. Severe Emphysema Treated by Endoscopic Bronchial Volume Reduction with Lung Sealant (AeriSeal

    Directory of Open Access Journals (Sweden)

    R. F. Falkenstern-Ge

    2013-01-01

    Full Text Available Endoscopic lung volume reduction using lung sealant is a very new and innovative treatment option for patients with severe progressive and irreversible lung emphysema. A 55-year-old ex-smoker (60 pack years referred to our center because of severe lung emphysema with progressive worsening of the obstructive ventilator pattern and clinical condition. We detected collateral channels of this patient by using the Chartis system. Therefore, we decided to treat the advanced emphysema of our patient with endoscopic volume reduction using lung sealant (AeriSeal. The foam of lung sealant AeriSeal is instilled into the peripheral airways and alveoli where it polymerizes and functions as tissue glue on the lung surface in order to seal the target region to cause durable irreversible absorption atelectasis. The follow-up evaluation 12 weeks later showed improved lung function (increased FEV 1/partial oxygen pressure/peripheral oxygen saturation and a reduction of TLC and RV with improved quality of life. Correlation between changes in primary and secondary outcome measures in the lung function parameters and 6-minute-walking test before and 12 weeks after the application of lung sealant revealed significant reduction of hyperinflation and improvement both in the flow rates and in the physical capability of this patient.

  9. In vitro study of Streptococcus mutans adhesion on composite resin coated with three surface sealants

    Science.gov (United States)

    Kim, Da Hye

    2017-01-01

    Objectives Although the coating of surface sealants to dental composite resin may potentially reduce bacterial adhesion, there seems to be little information regarding this issue. This preliminary in vitro study investigated the adhesion of Streptococcus mutans (S. mutans) on the dental composite resins coated with three commercial surface sealants. Materials and Methods Composite resin (Filtek Z250) discs (8 mm in diameter, 1 mm in thickness) were fabricated in a mold covered with a Mylar strip (control). In group PoGo, the surfaces were polished with PoGo. In groups PS, OG, and FP, the surfaces polished with PoGo were coated with the corresponding surface sealants (PermaSeal, PS; OptiGuard, OG; Fortify Plus, FP). The surfaces of the materials and S. mutans cells were characterized by various methods. S. mutans adhesion to the surfaces was quantitatively evaluated using flow cytometry (n = 9). Results Group OG achieved the lowest water contact angle among all groups tested (p 0.05) or significantly lower (group OG, p < 0.001) bacterial adhesion when compared with the control group. Conclusions The application of the surface sealants significantly reduced S. mutans adhesion to the composite resin polished with the PoGo. PMID:28194363

  10. Radon penetration of concrete slab cracks, joints, pipe penetrations, and sealants

    NARCIS (Netherlands)

    Nielson, KK; Rogers, VC; Holt, RB; Pugh, TD; Grondzik, WA; deMeijer, RJ

    1997-01-01

    Radon movement through 12 test slabs with different cracks, pipe penetrations, cold joints, masonry blocks, sealants, and tensile stresses characterized the importance of these anomalous structural domains, Diffusive and advective radon transport were measured with steady-state air pressure differen

  11. Synthesis and Application of a Novel Organosilane Modifier for Surface Paintability of Organosiloxane Sealants

    Institute of Scientific and Technical Information of China (English)

    Wen Fei WANG; Qiang ZHENG; Hong Guo HU; Wei Wei LIN

    2005-01-01

    A novel organosilane, N-n-butyl-γ-aminopropylmethyldimethoxysilane was synthesized through aminization reaction and the chemical structure of resulting products was characterized by 1HNMR, 13CNMR, FT-IR and elemental analysis. The results of test on probation for this organosilane proved that it was effective to modify surface-paintability of organosiloxane sealants.

  12. Morphology of sealant/enamel interface after surface treatment with bioactive glass.

    Science.gov (United States)

    Panzeri Pires-de-Souza, Fernanda de Carvalho; Silveira, Renata Espíndola; Abuna, Gabriel; Chinelatti, Michelle Alexandra; Alandia-Román, Carla Cecilia; Sinhoreti, Mario Alexandre Coelho

    2015-12-01

    The purpose of this study was to analyze, by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), the morphology of sealant/enamel interface after surface treatment with Biosilicate. Before pits and fissures sealing, the occlusal surfaces of 10 sound human molars were sectioned perpendicularly at the fissures in order to obtain three slices for each tooth. Slices were randomly assigned into three groups (n = 10) according to sealing protocol: Group 1- Acid etching + Biosilicate + glass ionomer-based sealant (Clinpro XT Varnish, 3M ESPE); Group 2- Acid etching + glass ionomer-based sealant (Clinpro XT Varnish, 3M ESPE); Group 3- No sealing. All slices were subjected to thermal cycling (5,000 cycles; 5-55°C; dwell time: 30s). Half of the slices from each group (n = 5) were analyzed by CLSM and the other half by SEM. Groups 1 and 2 were also submitted to EDS analysis and their data were evaluated by Two-Way ANOVA e Tukey's test (α=5%). EDS data analysis showed higher amounts of silicon (Si) ions than calcium (Ca) ions in Group 1 (P glass ionomer-based sealant/enamel interfaces.

  13. In vitro evaluation of shear bond strength and microleakage of different pit and fissure sealants

    Science.gov (United States)

    Babaji, Prashant; Vaid, Shivali; Deep, S.; Mishra, Samvit; Srivastava, Madhulika; Manjooran, Thomas

    2016-01-01

    Aim and Objectives: Fissure caries is most common in children due to deep pit and fissures. Pit and fissure areas on the occlusal surface of the teeth make them susceptible to dental caries, which need to be prevented or restored. Fissures sealant reduces the risk of occlusal caries. The present study was done to evaluate microleakage and shear bond strength of various fissure sealants. Materials and Methods: Thirty-six extracted molars were randomly allocated equally (n = 12) into three groups with three different sealants to evaluate shear bond strength and microleakage at sealant space. The shear bond strengths was evaluated with one-way analysis of variance and microleakage by Kruskal-Wallis nonparametric test using the Statistical Package for the Social Sciences version 18.0 (Chicago: SPSS Inc, 2009). Results: Tetric flow (16.8 MPa) recorded the highest shear bond strength and the difference was statistically significant with enamel loc (12.8 MPa). There was no statistically significant difference in relation to microleakage (P > 0.05) in the tested groups. Conclusions: Tetric flow recorded the highest shear bond strength and the difference was statistically significant with enamel loc. However, there was no statistically significant difference among the groups regarding microleakage. PMID:27652241

  14. 76 FR 64237 - Approval and Promulgation of Air Quality Implementation Plans; Maryland; Adhesives and Sealants Rule

    Science.gov (United States)

    2011-10-18

    ... pertains to amendments to Maryland's rule for the control of volatile organic compound (VOC) emissions from... regulation for the control of VOC emissions from adhesives and sealants. EPA is approving this SIP revision... miscellaneous industrial adhesives control techniques guideline (CTG) category in accordance with...

  15. Effects of aging on surface properties and adhesion of Streptococcus mutans on various fissure sealants.

    Science.gov (United States)

    Bürgers, Ralf; Cariaga, Tashiana; Müller, Rainer; Rosentritt, Martin; Reischl, Udo; Handel, Gerhard; Hahnel, Sebastian

    2009-12-01

    The aim of the present study was the quantification of Streptococcus mutans adhesion on ten widely used pit and fissure sealant materials and the correlation of these findings to surface roughness (R(a)) and surface free energy (SFE). Additionally, changes in streptococcal adhesion and surface parameters after water immersion and artificial aging have been investigated. Circular specimens of ten fissure sealants (seven resin-based composites, two glass ionomers, and one compomer) were made and polished. Surface roughness was determined by perthometer and SFE by goniometer measurements. Sealant materials were incubated with S. mutans suspension (2.5 h, 37 degrees C), and adhering bacteria were quantified by using a biofluorescence assay in combination with an automated plate reader. Surface properties and S. mutans adhesion were measured prior to and after water immersion after 1 and 6 months and after additional thermocycling (5,000 cycles; 5 degrees C/55 degrees C). The tested sealants showed significant differences in S. mutans adhesion prior to and after the applied aging procedures. Aging resulted in slight increases (mostly <0.2 microm) in surface roughness, as well as in significant decreases in SFE and in significantly lower quantities of adhering bacteria. Ketac Bond and UltraSeal XT plus revealed the lowest adhesion potential after artificial aging. In general, the amount of adhering S. mutans was reduced after aging, which may be related to the decline in SFEs.

  16. Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization.

    NARCIS (Netherlands)

    Lugovskoy, E.V.; Gritsenko, P.; Koshel, T.A.; Koliesnik, I.O.; Cherenok, S.O.; Kalchenko, O.I.; Kalchenko, V.I.; Komisarenko, S.V.

    2011-01-01

    Calix[4]arenes bearing two or four methylenebisphosphonic acid groups at the macrocyclic upper rim have been studied with respect to their effects on fibrin polymerization. The most potent inhibitor proved to be calix[4]arene tetrakis-methylene-bis-phosphonic acid (C-192), in which case the maximum

  17. Enzymatically induced mineralization of platelet-rich fibrin.

    NARCIS (Netherlands)

    Douglas, T.E.L.; Gassling, V.; Declercq, H.A.; Purcz, N.; Pamula, E.; Haugen, H.J.; Chasan, S.; Mulder, E.L.W. de; Jansen, J.A.; Leeuwenburgh, S.C.G.

    2012-01-01

    Membranes of the autologous blood-derived biomaterial platelet-rich fibrin (PRF) were functionalized by incorporation of alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, and subsequently incubated in calcium glycerophosphate (CaGP) solution to induce PRFs mineralization with

  18. PLATELET RICH FIBRIN: A PROMISING INNOVATION IN REGENERATIVE THERAPY

    Directory of Open Access Journals (Sweden)

    Arun

    2015-04-01

    Full Text Available Platelets can play a crucial role in regenerative therapy as they are reservoirs of growth factors and cytokines which are the key factors for regeneration of the bone and maturation of the soft tissue. Platelet - rich fibr in (PRF was first described by Choukroun et al. in France. It has been referred to as a second - generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Platelet - rich fibrin (PRF is autologous plate let concentrates prepared from patient’s own blood. It is a natural fibrin - based biomaterial prepared from an anticoagulant - free blood harvest without any artificial biochemical modification that allows obtaining fibrin membranes enriched with platelets a nd growth factors. Evidence from the literature suggests the potential role of PRF in regeneration and tissue engineering. The slow polymerisation during centrifugation and fibrin - based structure makes PRF a better healing biomaterial than PRP and other fi brin adhesives. The purpose of this review article is to describe the novel second - generation platelet concentrate PRF, which is an improvement over the traditionally prepared PRP for use in regenerative dentistry.

  19. Effect of Acidulated Phosphate Fluoride (APF on the Mcroleakage of Composite Flow and Fssure Sealant Restorations

    Directory of Open Access Journals (Sweden)

    Arash Poorsattar Bejeh Mir

    2012-09-01

    Full Text Available Background and Aims: A large number of investigations have revealed that physical and chemical alterations and weight loss could occur in composite materials exposed to acidic phosphate fluoride (APF gel. The purpose of this study was to assess the microleakage of a fissure sealant and a flow composite exposed to acidulated phosphate fluoride (APF gel. Materials and Methods: In this in vitro study, 60 intact human extracted premolar teeth were used. After preparing the occlusal groove, the teeth were divided into two groups (n=30. Teeth were filled with either Helioseal fissure sealant (Vivadent, Germany or Tetric flow composite (Vivadent, Germany. After that, each group was divided into two subgroups (n=15: 1.23% APF gel (Sultan, U.S.A was applied in the case subgroups, while control subgroups were preserved in normal saline solution. All of teeth were covered with 2 layers of nail varnish except for the filling zone and 1mm around the border of filling. After submerging in 0.5% fushin solution, specimens were sectioned bucco-lingually. Then dye penetration through the filling and fissure sealant was assessed by means of a stereo-microscope. The depth of dye penetration was scored. The data were analyzed using One-way ANOVA and Levene test. Results: The mean values of dye penetration were 1.26±1.09, 1.4±1.05, 1.2±1.37, and 1.4±1.35 for fissure sealant+gel, composite+gel, composite+normal saline, and normal saline groups, respectively. No significant difference was found in inter-groups (P=0.96. Conclusion: Considering the result of the present research, APF gel had no significant effect on the microleakage of Tetric flow composite filling and Helioseal fissure sealant and thus, it can be applied for routine usage.

  20. Concentration independent modulation of local micromechanics in a fibrin gel.

    Directory of Open Access Journals (Sweden)

    Maxwell A Kotlarchyk

    Full Text Available Methods for tuning extracellular matrix (ECM mechanics in 3D cell culture that rely on increasing the concentration of either protein or cross-linking molecules fail to control important parameters such as pore size, ligand density, and molecular diffusivity. Alternatively, ECM stiffness can be modulated independently from protein concentration by mechanically loading the ECM. We have developed a novel device for generating stiffness gradients in naturally derived ECMs, where stiffness is tuned by inducing strain, while local mechanical properties are directly determined by laser tweezers based active microrheology (AMR. Hydrogel substrates polymerized within 35 mm diameter Petri dishes are strained non-uniformly by the precise rotation of an embedded cylindrical post, and exhibit a position-dependent stiffness with little to no modulation of local mesh geometry. Here we present the device in the context of fibrin hydrogels. First AMR is used to directly measure local micromechanics in unstrained hydrogels of increasing fibrin concentration. Changes in stiffness are then mapped within our device, where fibrin concentration is held constant. Fluorescence confocal imaging and orbital particle tracking are used to quantify structural changes in fibrin on the micro and nano levels respectively. The micromechanical strain stiffening measured by microrheology is not accompanied by ECM microstructural changes under our applied loads, as measured by confocal microscopy. However, super-resolution orbital tracking reveals nanostructural straightening, lengthening, and reduced movement of fibrin fibers. Furthermore, we show that aortic smooth muscle cells cultured within our device are morphologically sensitive to the induced mechanical gradient. Our results demonstrate a powerful cell culture tool that can be used in the study of mechanical effects on cellular physiology in naturally derived 3D ECM tissues.

  1. Retention of a Flowable Composite Resin in Comparison to a Conventional Resin-Based Sealant: One-year Follow-up

    Directory of Open Access Journals (Sweden)

    N. Tadayon

    2010-03-01

    Full Text Available Objective: Long-term retention of pit and fissure sealants is crucial for their success. This clinical study evaluated the retention rate of a flowable composite resin (Filtek SupremeXT Flowable Restorative compared to a conventional resin-based sealant (Concise Light Cure White Sealant over 12 months.Materials and Methods: Forty subjects aged 6 to 9 years were included in the study. Using a half-mouth design, a total of 80 first permanent molars were sealed with conventional fissure sealant on one side of the mouth and flowable composite on the contralateralside. Clinical evaluation was performed at 3, 6, and 12 months by a single blind examiner and the retention was classified as complete retention, partial loss, or total loss.Results: For both materials, there was no total loss of sealants over 12 months. Partial loss of both materials was observed in one sealant after 3 months. After 6 months, 36 teeth sealed with conventional fissure sealant were intact compared with 37 sealed with a flowable composite, and after 12 months, 33 teeth sealed with conventional fissure sealant were intact compared with 35 that were sealed with a flowable composite. There were no statistically significant difference (P>0.05 between the two materials regarding the retention rate at each follow-up period.Conclusion: As flowable composite resulted in comparable sealant retention rates, this material could be a good choice for fissure sealant.

  2. 医用生物蛋白胶在胸外科手术中的应用%The Application of Medical Fibrin Sealant In Thoracic Surgery

    Institute of Scientific and Technical Information of China (English)

    黄涛

    2007-01-01

    目的:观察医用生物蛋白胶对胸外科手术术后引流量的影响.方法:对同期胸外科手术实验组55人及观察组53人进行术后24h引流量及引流总量进行观察比较.结果:使用医用生物蛋白胶的实验组术后24h引流量及引流总量明显少于观察组(P<0.05).结论:医用生物蛋白胶可有效减少胸外科手术的术后24h引流量及引流总量,促进术后恢复.

  3. 纤维蛋白封闭剂在甲状腺手术中的应用%Application of Fibrin Sealant in Thyroid surgery

    Institute of Scientific and Technical Information of China (English)

    劳卫荣; 梁国健; 林建生

    2009-01-01

    目的 探讨纤维蛋白封闭剂在甲状腺手术中的应用效果.方法 以行双侧甲状腺次全切除术的患者为研究对象,将2007年1月~2008年3月在广州医学院第二附属医院行此手术治疗的115例患者随机分成A、B两组,A组术中应用纤维蛋白封闭刺,B组不用.术后两组患者进行引流量等多项指标比较.结果 A组的伤口引流量、切口拆线时间等多项指标均优于B组.结论 在甲状腺手术中应用纤维蛋白封闭剂术后创面渗出液明显减少,可促进伤口愈合,有应用价值.

  4. Elastic behavior and platelet retraction in low- and high-density fibrin gels.

    Science.gov (United States)

    Wufsus, Adam R; Rana, Kuldeepsinh; Brown, Andrea; Dorgan, John R; Liberatore, Matthew W; Neeves, Keith B

    2015-01-06

    Fibrin is a biopolymer that gives thrombi the mechanical strength to withstand the forces imparted on them by blood flow. Importantly, fibrin is highly extensible, but strain hardens at low deformation rates. The density of fibrin in clots, especially arterial clots, is higher than that in gels made at plasma concentrations of fibrinogen (3-10 mg/mL), where most rheology studies have been conducted. Our objective in this study was to measure and characterize the elastic regimes of low (3-10 mg/mL) and high (30-100 mg/mL) density fibrin gels using shear and extensional rheology. Confocal microscopy of the gels shows that fiber density increases with fibrinogen concentration. At low strains, fibrin gels act as thermal networks independent of fibrinogen concentration. Within the low-strain regime, one can predict the mesh size of fibrin gels by the elastic modulus using semiflexible polymer theory. Significantly, this provides a link between gel mechanics and interstitial fluid flow. At moderate strains, we find that low-density fibrin gels act as nonaffine mechanical networks and transition to affine mechanical networks with increasing strains within the moderate regime, whereas high-density fibrin gels only act as affine mechanical networks. At high strains, the backbone of individual fibrin fibers stretches for all fibrin gels. Platelets can retract low-density gels by >80% of their initial volumes, but retraction is attenuated in high-density fibrin gels and with decreasing platelet density. Taken together, these results show that the nature of fibrin deformation is a strong function of fibrin fiber density, which has ramifications for the growth, embolization, and lysis of thrombi.

  5. Use of occlusal sealant in a community program and caries incidence in high- and low-risk children

    Directory of Open Access Journals (Sweden)

    Vânia Baldini

    2011-08-01

    Full Text Available OBJECTIVE: The aims of this study were to investigate the effectiveness of sealant placement under the guidelines of the Oral Health Promotion Program for Children and Adolescents (Portugal, and to test the influence of clinical and socioeconomic variables on the DMFT increment in 277 children, born in 1997. MATERIAL AND METHODS: A dental hygienist performed the initial examinations and sealant placement (Helioseal, Vivadent on the permanent first molars in 2005. These activities were registered in dental records that were assessed in 2007. Children were classified according to caries risk at baseline [high (HR: DMFT+dmft>0; low (LR: DMFT+dmft=0 risk] and sealant placement as follows: HR-S and LR-S Groups (with sealant placement; HR-NS and LR-NS Groups (without sealant placement. A calibrated dentist performed the final examination in 2007 at school, based on the World Health Organization recommendations. The variables collected were: dental caries, visible dental plaque, malocclusions, and socioeconomic level (questionnaire sent to children's parents. For univariate (Chi-square or Fisher tests and multivariate (Multiple logistic regression analyses the DMFT increment >0 was selected as dependent variable. RESULTS: Approximately 17.0% of the children showed DMFT increment>0 (mean=0.25. High-risk children presented a significant increase in the number of decayed and/or filled teeth. These children had 7.94 more chance of developing caries. Children who did not receive sealant were 1.8 more prone to have DMFT increment >0. CONCLUSION: It appears that sealant placement was effective in preventing dental caries development. Moreover, the variables "risk" and "sealant placement" were predictors for DMFT increment in the studied children.

  6. The mechanics of fibrin networks and their alterations by platelets

    Science.gov (United States)

    Jawerth, Louise Marie

    Fibrin is a biopolymer that assembles into a network during blood coagulation to become the structural scaffold of a blood clot. The precise mechanics of this network are crucial for a blood clot to properly stem the flow of blood at the site of vascular injury while still remaining pliable enough to avoid dislocation. A hallmark of fibrin's mechanical response is strain-stiffening: at small strains, its response is low and linear; while at high strains, its stiffness increases non-linearly with increasing strain. The physical origins of strain-stiffening have been studied for other biopolymer systems but have remained elusive for biopolymer networks composed of stiff filaments, such as fibrin. To understand the origins of this intriguing behavior, we directly observe and quantify the motion of all of the fibers in the fibrin networks as they undergo shear in 3D using confocal microscopy. We show that the strain-stiffening response of a clot is a result of the full network deformation rather than an intrinsic strain-stiffening response of the individual fibers. We observe a distinct transition from a linear, low-strain regime, where all fibers avoid any internal stretching, to a non-linear, high-strain regime, where an increasing number of fibers become stretched. This transition is characterized by a high degree of non-affine motion. Moreover, we are able to precisely calculate the non-linear stress-strain response of the network by using the strains on each fiber measured directly with confocal microscopy and by assuming the fibers behave like linearly elastic beams. This result confirms that it is the network deformation that causes the strain-stiffening behavior of fibrin clots. These data are consistent with predictions for low-connectivity networks with soft, bending, or floppy modes. Moreover, we show that the addition of small contractile cells, platelets, increases the low-strain stiffness of the network while the high-strain stiffness is independent of

  7. Experimental Study of the Aging and Self-Healing of Glass/Ceramic Sealant Used in SOFCs

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Wenning N.; Sun, Xin; Koeppel, Brian J.; Khaleel, Mohammad A.

    2010-01-01

    High operating temperatures of solid oxide fuel cells (SOFCs) require that sealant must function at a high temperature between 600oC and 900oC and in the oxidizing and reducing environments of fuel and air. This paper describes tests to investigate the temporal evolution of the volume fraction of ceramic phases, the evolution of micro-damage, and the self-healing behavior of the glass ceramic sealant used in SOFCs. It was found that after the initial sintering process, further crystallization of the glass ceramic sealant does not stop, but slows down and reduces the residual glass content while boosting the ceramic crystalline content. Under the long-term operating environment, distinct fibrous and needle-like crystals in the amorphous phase disappeared, and smeared/diffused phase boundaries between the glass phase and ceramic phase were observed. Meanwhile, the micro-damage was induced by the cooling-down process from the operating temperature to the room temperature, which can potentially degrade the mechanical properties of the glass/ceramic sealant. The glass/ceramic sealant self-healed upon reheating to the SOFC operating temperature, which can restore the mechanical performance of the glass/ceramic sealant.

  8. Flowable resin used as a sealant in molars using conventional, enameloplasty and fissurotomy techniques: An in vitro study

    Directory of Open Access Journals (Sweden)

    Chaitra T

    2010-09-01

    Full Text Available Background and Objective: Preventive procedures using pit and fissure sealants are one of the most important aspects of pediatric dental practice. The objectives of this in vitro study were to comparatively evaluate microleakage of a flowable resin used as a sealant on molars after preparation with conventional, enameloplasty and fissurotomy techniques. Materials and Methods: A total of 24 teeth were divided into three groups of eight samples each. Pits and fissures were prepared with conventional sealant technique (CST (Group A, enameloplasty sealant technique (EST (Group B and fissurotomy technique (FT (Group C. Following sealant placement, thermocycling and sectioning of samples of Groups A, B and C were performed and microleakage was assessed under a stereomicroscope after methylene blue dye immersion. Results: There was significantly lesser microleakage in EST when compared with CST and FT, which showed more microleakage. No statistical significance between CST and FT was found. Interpretation and Conclusion: EST has proven to be an excellent method of preparation of pits and fissures when flowable composite is used as a sealant because of its lesser microleakage.

  9. PAH volatilization following application of coal-tar-based pavement sealant

    Science.gov (United States)

    Van Metre, Peter C.; Majewski, Michael S.; Mahler, Barbara J.; Foreman, William T.; Braun, Christopher L.; Wilson, Jennifer T.; Burbank, Teresa L.

    2012-01-01

    Coal-tar-based pavement sealants, a major source of PAHs to urban water bodies, have recently been identified as a source of volatile PAHs to the atmosphere. We tracked the volatilization of PAHs for 1 year after application of a coal-tar-based pavement sealant by measuring gas-phase PAH concentrations above the pavement surface and solid-phase PAH concentrations in sealant scraped from the surface. Gas-phase concentrations at two heights (0.03 and 1.28 m) and wind speed were used to estimate volatilization flux. The sum of the concentrations of eight frequently detected PAHs (ΣPAH8) in the 0.03-m sample 1.6 h after application (297,000 ng m-3) was about 5000 times greater than that previously reported for the same height above unsealed parking lots (66 ng m-3). Flux at 1.6 h after application was estimated at 45,000 μg m-2 h-1 and decreased rapidly during the 45 days after application to 160 μg m-2 h-1. Loss of PAHs from the adhered sealant also was rapid, with about a 50% decrease in solid-phase ΣPAH8 concentration over the 45 days after application. There was general agreement, given the uncertainties, in the estimated mass of ΣPAH8 lost to the atmosphere on the basis of air sampling (2–3 g m-2) and adhered sealant sampling (6 g m-2) during the first 16 days after application, translating to a loss to the atmosphere of one-quarter to one-half of the PAHs in the sealcoat product. Combining the estimated mass of ΣPAH8 released to the atmosphere with a national-use estimate of coal-tar-based sealant suggests that PAH emissions from new coal-tar-based sealcoat applications each year (~1000 Mg) are larger than annual vehicle emissions of PAHs for the United States.

  10. 有机硅密封剂高温力学性能研究%Study on mechanical properties of silicone sealants at high temperature sealant

    Institute of Scientific and Technical Information of China (English)

    冯志力; 周文胜; 孙全吉; 张鹏; 王恒芝; 范召东

    2014-01-01

    The tensile strength,180° peel strength and shear strength of five silicone sealants obtained with three vulcanization systems at the temperature range of 25°C to 250°C.The results showed that with increasing temperature,the tensile strength,180º peel strength and shear strength of the five silicone sealants were declining,but the rate of reduction of tensile strength and shear strength was declining.The mechanical properties of silicone sealants are closely related to the type of vulcanization system.The tensile strength,180º peel strength and shear strength of HM321 sealant are the highest at the temperature range of 25°C to 150°C,and the retention rate of tensile strength,180º peel strength and shear strength of HM321 are the lowest at the temperature range of 25°C to 250°C.The tensile strength and shear strength of XY602S sealant are the highest at the temperature range of 232°C to 250°C,and the retention rate of tensile strength of XY602S sealant are the highest too at the temperature range of 25°C to 250°C.%在温度25~250℃对3类硫化体系的5种有机硅密封剂的拉伸强度、180º剥离强度和剪切强度的研究表明:随着温度的升高,5种有机硅密封剂的拉伸强度、180º剥离强度和剪切强度均不断下降,其中拉伸强度和剪切强度降低的速率递减。有机硅密封剂的高温性能变化与硫化体系类型密切相关,脱氢硫化体系HM321密封剂在25~150℃拉伸强度、剪切强度和180º剥离强度均最高,但在25~250℃拉伸强度、剪切强度和180º剥离强度保持率均最低。脱氨硫化体系XY-602S有机硅胶粘剂在232~250℃的拉伸强度和剪切强度最高,25~250℃的拉伸强度保持率也最高。

  11. Retention of a Flowable Composite Resin in Comparison to a Conventional Resin-Based Sealant: One-year Follow-up

    OpenAIRE

    N. Tadayon; B. Malekafzali; M.Jafarzadeh; Fallahi, S.

    2010-01-01

    Objective: Long-term retention of pit and fissure sealants is crucial for their success. This clinical study evaluated the retention rate of a flowable composite resin (Filtek Supreme XT Flowable Restorative) compared to a conventional resin-based sealant (Concise Light Cure White Sealant) over 12 months. Materials and Methods: Forty subjects aged 6 to 9 years were included in the study. Using a half-mouth design, a total of 80 first permanent molars were sealed with conventional fissure seal...

  12. A Novel Role for Erythropoietin During Fibrin-Induced Wound-Healing Response

    OpenAIRE

    Haroon, Zishan A.; Amin, Khalid; Jiang, XiaoHong; Arcasoy, Murat O.

    2003-01-01

    In this study, we investigated the role of the hematopoietic cytokine erythropoietin (EPO) during wound healing, the physiological response to tissue injury. We used an in vivo wound-healing assay (fibrin Z-chambers) consisting of fibrin-filled chambers implanted subcutaneously in rats. The fibrin inside the chambers is replaced by granulation tissue consisting of new blood vessels, macrophages and fibroblasts as part of the wound-healing response. Local, exogenous recombinant EPO administrat...

  13. Platelet-rich fibrin matrix for facial plastic surgery.

    Science.gov (United States)

    Sclafani, Anthony P; Saman, Masoud

    2012-05-01

    Platelets are known primarily for their role in hemostasis, but there is increasing interest in the effect of platelets on wound healing. Platelet isolates such as platelet-rich plasma have been advocated to enhance and accelerate wound healing. This article describes the use of a novel preparation, platelet-rich fibrin matrix (PRFM), for facial plastic surgery applications such as volume augmentation, fat transfer supplementation, and as an adjunct to open surgical procedures.

  14. [Application of fibrin glue in facial nerve repair].

    Science.gov (United States)

    Wang, Qinying; Hua, Qingquan; Wang, Shenqing

    2007-06-01

    This animal experiment was aimed to apply fibrin in facial nerve repair and to quest for technical improvements in facial surgery. In each of 15 healthy large ear white rabbits, a unilateral 5 mm intratemporal facial nerve gap was created, the proximal and distal stumps were inserted into chitin tube, 1 ml autologous fibrin glue was applied around the anastomotic zone, and no suture was employed. At 3 months and 5 months after opertion, electrophysioligical study was performed. Compared with normal nerves, the regenerating nerves in both the chitin tube bridged group and the perineurium suture group had longer incubation period, lower amplitude, slower nerve-muscle conduction velocity at 3 months postoperatively. The differences were distinctly significant (P < 0.01). Although being decreased at 5 months after operation, the differences were still statistically significant (P < 0.05). There were no significant differences between the chitin tube bridged group and perineurium suture group at 3 months and 5 months, respectively. The study suggests that facial nerve repair using fibrin glue and chitin tube has the advantages of being easier,faster and more stable.

  15. Fiber optic immunosensor for cross-linked fibrin concentration

    Science.gov (United States)

    Moskowitz, Samuel E.

    2000-08-01

    Working with calcium ions in the blood, platelets produce thromboplastin which transforms prothrombin into thrombin. Removing peptides, thrombin changes fibrinogen into fibrin. Cross-linked insoluble fibrin polymers are solubilized by enzyme plasmin found in blood plasma. Resulting D-dimers are elevated in patients with intravascular coagulation, deep venous thrombosis, pulmonary embolism, myocardial infarction, multiple trauma, cancer, impaired renal and liver functions, and sepsis. Consisting principally of a NIR 780 nm GaAlAs laser diode and a 800 nm avalanche photodiode (APD), the fiber-optic immunosensor can determined D-dimer concentration to levels detected by a second antibody which is labeled with NN 382 fluorescent dye. An evanescent wave traveling on an excitation optical fiber excites the antibody-antigen fluorophore complex. Concentration of cross-linked fibrin is directly proportional to the APD measured intensity of fluorescence. NIR fluorescence has advantages of low background interference, short fluorescence lifetime, and large difference between excitation and emission peaks. Competitive ELISA test for D-dimer concentration requires trained personnel performing a time consuming operation.

  16. An automated method for fibrin clot permeability assessment.

    Science.gov (United States)

    Ząbczyk, Michał; Piłat, Adam; Awsiuk, Magdalena; Undas, Anetta

    2015-01-01

    The fibrin clot permeability coefficient (Ks) is a useful measure of porosity of the fibrin network, which is determined by a number of genetic and environmental factors. Currently available methods to evaluate Ks are time-consuming, require constant supervision and provide only one parameter. We present an automated method in which drops are weighed individually, buffer is dosed by the pump and well defined clot washing is controlled by the software. The presence of a straight association between drop mass and their dripping time allows to shorten the measurement time twice. In 40 healthy individuals, Ks, the number of drops required to reach the plateau (DTP), the time to achieve the plateau (TTP) and the DTP/TTP ratio (DTR) were calculated. There was a positive association between Ks (r = 0.69, P Ks (r = -0.55, P Ks (r = 0.70, P < 0.0001 for the manual method and r = 0.76, P < 0.0001 for the automated method), fibrinogen (r = -0.58, P < 0.0001) and C-reactive protein (CRP) (r = -0.47, P < 0.01). The automated method might be a suitable tool for research and clinical use and may offer more additional parameters describing fibrin clot structure.

  17. Platelet rich fibrin: a new paradigm in periodontal regeneration.

    Science.gov (United States)

    Kumar, R Vinaya; Shubhashini, N

    2013-09-01

    Among the great challenges facing clinical research is the development of bioactive surgical additives regulating inflammation and increasing healing. Although the use of fibrin adhesives and platelet-rich plasma (PRP) is well documented, they have their own limitations. Hence, reconstructive dental surgeons are looking for an "edge" that jump starts the healing process to maximize predictability as well as the volume of regenerated bone. Overcoming the restrictions related to the reimplantation of blood-derived products, a new family of platelet concentrate, which is neither a fibrin glue nor a classical platelet concentrate, was developed in France. This second generation platelet concentrate called platelet-rich fibrin (PRF), has been widely used to accelerate soft and hard tissue healing. Its advantages over the better known PRP include ease of preparation/application, minimal expense, and lack of biochemical modification (no bovine thrombin or anticoagulant is required). This article serves as an introduction to the PRF "concept" and its potential clinical applications with emphasis on periodontal regeneration.

  18. A PEGylated Fibrin-Based Wound Dressing with Antimicrobial and Angiogenic Activity

    Science.gov (United States)

    2011-04-13

    CSM–PEGylated fibrin gel showing the uniform dispersion of SSD–CSM. (C) Rheology data shows that the PEGylated fibrin gel and SSD–CSM–PEGylated...and SSD–CSM in PEGylated fibrin gels (±SD) over a 72 h period (A) using a Franz apparatus or (B) under sink conditions. (C) Rheology data indicate...PEGylated fibrin gel Angiogenesis Adipose-derived stem cells a b s t r a c t Wounds sustained under battlefield conditions are considered to be contaminated

  19. Quality Assessment of Information About Pit and Fissure Sealants in Persian Websites in 2012

    Directory of Open Access Journals (Sweden)

    Firoozeh Nilchian Nilchian

    2016-08-01

    Full Text Available Objectives: Despite the increasing use of Internet, there is no supervision over the accuracy and quality of the information provided in the web. To deal with this problem, health specialists should take part in planning, publishing and supervision of online health-related information. The aim of this study was to evaluate the quality of information related to pit and fissure sealants in Persian websites.Materials and Methods: In this cross-sectional study, Persian websites providing information about fissure sealants were found using Google search engine. The searched keywords according to the MeSH database were "patient education" and "fissure sealant". After applying the exclusion criteria, 37 websites out of 500 initial links remained in the study. These websites were evaluated based on a researcher-made checklist. The validity and reliability of the checklist were evaluated and confirmed. Descriptive analysis was applied to report the results of our study using SPSS version 11.5.Results: The average score for the quality of information was 22.46 out of 38. The minimum scores were 16 and 30 and belonged to Pezeshkanemrooz.com and Asa85.blogfa.com, respectively. The results showed that 62.2% of the answers were scored 2-4 and 37.8% were scored 1; therefore, the overall quality of the published content was rated to be moderate for 62.2% and low for 37.8% of the websites.Conclusions: Overall, the quality of information related to fissure sealant provided in Persian websites was good; however, the information given was mostly incomplete and could be improved. The main problems were doubtful credibility and outdated information.

  20. Effect of Two Surface Sealants on Microleakage of Class V Resin Composite Restorations

    Directory of Open Access Journals (Sweden)

    Mosa Aboali

    2012-02-01

    Full Text Available Background and Aims: When composite resin polymerizes, shrinkage stresses tend to produce gaps at the tooth/ restoration interfaces. Surface sealants may reduce or avoid problems related to the marginal interface. The aim of this study was to evaluate the effect of two different surface sealants (Fortify and Optiguard on the microleakage of class V resin composite restorations. Materials and Methods: Twenty three sound noncarious molars were collected. Totally, 45 Class V cavities with the occlusal margins in enamel and cervical margins in cementum were prepared in both buccal and lingual surfaces. The specimens were randomly assigned in three groups (15 cavities in each group and then restored with a resin composite. After the finishing and polishing procedures, the restorations in each group were covered with a specific surface sealant, except for the control samples, which were not sealed. After placing restorations, the specimens were thermocycled and then immersed in a 50% silver nitrate solution (tracer agent for four hours, sectioned longitudinally and analyzed for leakage using a stereomicroscope in a blind manner. The marginal microleakage was evaluated at the occlusal and cervical interfaces and compared among the three groups using the Kruskall-Wallis and the Mann-Whitney U tests. Results: Microleakage was found in all groups at both occlusal and cervical margins. Significantly greater leakage was observed at the cervical margins compared to the enamel margins of the material groups (P=0.005. There was no statistically significant difference among the groups at occlusal margins (P=0.66. In the cervical region, Fortify showed improved results and statistically presented the lowest degree of microleakage (P=0.003. onclusion: The used sealant materials presented different rates of effectiveness and Fortify decreased marginal microleakage significantly.

  1. An evaluation of nanocomposites as pit and fissure sealants in child patients

    Directory of Open Access Journals (Sweden)

    S Singh

    2011-01-01

    Full Text Available Background: Dental caries affects populations of all ages in all the regions of the world, with the child patient being at the highest risk. Fifty percent of the permanent molars are decayed by the age of 12, due to lack of information about protection and occlusal morphology. Pit and fissure sealing undoubtedly plays a fundamental role in preventing occlusal caries. The most common approach to assess the sealing ability of pit and fissure sealants of tooth/restoration is the measurement of dye penetration along the interface. Aims and Objectives: The present study aims to evaluate the microleakage and penetration depth of three different types of dental materials, namely (A Conventional pit and fissure sealant, (B Flowable composite, (C Flowable nanocomposite. Study Design: This in-vitro comparative study comprised of extracted human posterior teeth mounted as blocks. Materials and Methods: In the present study, microleakage and penetration depths of conventional sealants/flowable composite and nanocomposite were measured with the help of a dye under stereomicroscope. Statistical Analysis: A student′t′ test and Analysis of variance (ANOVA tests were performed to compare the mean microleakage and penetration depth. Results: Microleakage was found to be highest for the flowable composites, and least for the conventional sealant. The nanocomposite values were intermediate. Penetration depth was highest for nanocomposite and least for flowable composite. Conclusions: According to the results, the nanocomposite was found to be an excellent dental material for penetration in deep pits and fissures, though it exhibits mild microleakage. Hence, it can be recommended for use in pediatric dental patients, as a pit and fissure sealing agent.

  2. Effectiveness of fluoride sealant in the prevention of carious lesions around orthodontic brackets: an OCT evaluation

    Directory of Open Access Journals (Sweden)

    Matheus Melo Pithon

    2015-12-01

    Full Text Available Abstract Objective: This article aimed to evaluate in vitro the efficiency of Pro Seal fluoride sealant application in the prevention of white spot lesions around orthodontic brackets. Material and Methods: Brackets were bonded to the buccal surface of bovine incisors, and five groups were formed (n = 15 according to the exposure of teeth to oral hygiene substances and the application of enamel sealant: G1 (control, only brushing was performed with 1.450 ppm fluoride; G2 (control brushing associated with the use of mouthwash with 225 ppm fluoride; G3, only Pro Seal sealant application was performed with 1.000 ppm fluoride; G4 Pro Seal associated with brushing; G5 Pro Seal associated with brushing and mouthwash. Experimental groups alternated between pH cycling and the procedures described. All specimens were kept at a temperature of 37 °C throughout the entire experiment. Both brushing and immersion in solutions were performed within a time interval of one minute, followed by washing in deionized water three times a day for 28 days. Afterwards, an evaluation by Optical Coherence Tomography (OCT of the spectral type was performed. In each group, a scanning exam of the white spot lesion area (around the sites where brackets were bonded and depth measurement of carious lesions were performed. Analysis of variance (ANOVA was applied to determine whether there were significant differences among groups. For post hoc analysis, Tukey test was used. Results: There was statistically significant difference between groups 1 and 2 (p = 0.003, 1 and 3 (p = 0.008, 1 and 4 (p = 0.000 and 1 and 5 (p = 0.000. The group in which only brushing was performed (Group 1 showed deeper enamel lesion. Conclusion: Pro Seal sealant alone or combined with brushing and/or brushing and the use of a mouthwash with fluoride was more effective in protecting enamel, in comparison to brushing alone.

  3. THE EFFECT OF BONDING AND SURFACE SEALANT APPLICATION ON POSTOPERATIVE SENSITIVITY FROM POSTERIOR COMPOSITES

    Directory of Open Access Journals (Sweden)

    Neslihan TEKÇE

    2015-10-01

    Full Text Available Purpose: The purpose of the study was to evaluate the postoperative sensitivity of posterior Class I composite restoration at short-term, restorated with two different all-in-one self-etch adhesives with or without surface sealant application. Materials and Methods: 44 restorations were inserted in 11 patients who required Class I restorations in their molars. Each patient received 4 restorations, thus four groups were formed; (1 G-Aenial Bond (GC, Japan; (2 Clearfil S3 Bond (Kuraray, Japan; (3 G-Aenial Bond+Fortify Plus (Bisco, USA, (4 Clearfil S3 Bond+Fortify Plus. Sensitivity was evaluated at 24h, 7, 15, and 30 days using cold air, ice, and pressure stimuli using a visual analog scale. Comparisons of continuous variables between the sensitivity evaluations were performed using the Friedman’s One-Way Analysis of Variance with repeated measures test (p0.05. The use of Clearfil S3 Bond resulted in almost the same level of postoperative sensitivity as did the use of G-Aenial Bond. The highest sensitivity scores were observed for the surface sealant applied teeth without any statistical significance (p>0.05. Conclusions: Self etch adhesives displayed postoperative sensitivity. The sensitivity scores slightly decreased at the end of 30 days (p>0.05. Surface sealant application did not result in a decrease in sensitivity scores for either dentin adhesives.

  4. Characterization and the Pattern of Surfaces of Sealant with nano size Composite Materials

    Science.gov (United States)

    Quddos, A.; Samtio, N. H.; Syed, A. M.

    2013-06-01

    Nano composite sealant is low viscosity, room temperature cured, opaque and flowable nature. They have variety of uses such as potting, pressure sealant and shock resistant. Most important factor influencing use of fillers in polymer composites is their ability to effectively transfer the applied load in the matrix. The effective utilization of fillers in composites for structural applications depends strongly on the ability to disperse the nano fillers homogeneously in the matrix without damaging them. R-Belite supper epoxy adhesive (RBSEA) were formulated with different nano fillers (KCl, Al2O3, ZrO2, SiO2, ZrO2) at room temperature. The composite were prepared with the 0.02 to 0.10 weight ratios to promote the nucleation of the nanoparticles in the applied sealant. Two main problems which arise in improving the properties are poor dispersion of the fillers in the composite and weak bonding between nano fillers and the matrix. These problems are solved by mechanical and chemical means. It was observed that mechanical properties like tensile strength, elongation hardness etc and thermal properties were also improved with incorporation of nanofillers in the working applied polymer matrix. The dispersion of nano fillers in polymer matrix is studied by Scanning electron microscopy (SEM). The results confirm the presence of nanomaterial in RBSEA/fillers nanocomposites. SEM is also used to characterize the pattern of surfaces with nano size composite materials.

  5. Comparative study of fluoride released and recharged from conventional pit and fissure sealants versus surface prereacted glass ionomer technology

    Directory of Open Access Journals (Sweden)

    Elias Nahum Salmerón-Valdés

    2016-01-01

    Full Text Available Context: The fluoride release of sealants in vitro shows a marked decrease. Giomers are distinguishable from manufactured resin-based sealants and contain prereacted glass-ionomer particles (PRG. Aims: To compare the amounts of fluoride released from the main pit and fissure of a resin-based sealant with that from a Giomer and to assess the abilities of the sealant and the Giomer to recharge when exposed to regular use of fluoride rinse. Materials and Methods: The readings for the fluoride concentration were carried out for 60 days using a fluoride ion-specific electrode. After this period, the samples were recharged using a fluoride mouth rinse. The amount of fluoride released after this recharge was determined for 5 days. The data were analyzed using Student′s t- and analysis of variance tests. Results: In general, all materials presented higher fluoride release in the first 24 h; G1 and G4 showed a higher fluoride release in this period. On the other hand, G3 and G1 presented the most constant fluoride release until the 8 th day, wherein all the sealants considerably decreased in the amount of fluoride released. Conclusion: G1 and G3 released higher concentrations of fluoride, although no significant differences were found. Giomers recharged in the first 24 h after polymerization presented an improved and sustained fluoride release.

  6. Micro-leakage of a Fissure Sealant Cured Using Quartz-tungsten-halogen and Plasma Arc Light Curing Units.

    Science.gov (United States)

    Bahrololoomi, Zahra; Soleimani, Ali Asghar; Jafari, Najmeh; Varkesh, Bentolhoda

    2014-01-01

    Background and aims. Newer curing units such as plasma arc can polymerize the sealants in much shorter curing times. The aim of this study was to compare the effect of two different curing units on the micro-leakage of a fissure sealant material. Materials and methods. Sixty two extracted premolars without caries were randomly divided into two groups of 31 samples. Occlusal surfaces of all teeth were cleansed. Then, teeth surfaces were etched by 37% phosphoric acid. After rinsing and drying, occlusal surfaces of teeth were sealed by a fissure sealant. The sealant was then cured using either a halogen light curing unit or a plasma arc curing light. After sealing, the teeth were thermocycled for 500 cycles. The teeth were then sectioned and examined for micro-leakage. Statistical analyses were performed with Mann-Whitney test. Results. There was no significant difference between two groups regarding micro-leakage (P = 0.42). Conclusion. Results showed that there was no significant difference between two different curing units. Therefore, plasma arc unit might be a useful alternative for sealant polymerization.

  7. Anti-fibrin antibody binding in valvular vegetations and kidney lesions during experimental endocarditis.

    Science.gov (United States)

    Yokota, M; Basi, D L; Herzberg, M C; Meyer, M W

    2001-01-01

    In Streptococcus sanguinis (sanguis) induced experimental endocarditis, we sought evidence that the development of aortic valvular vegetation depends on the availability of fibrin. Endocarditis was induced in New Zealand white rabbits by catheter placement into the left ventricle and inoculation of the bacteria. Fibrin was localized in the developing vegetation with 99mTechnetium (Tc)-labeled anti-fibrin antibody one or three days later. When rabbit anti-fibrin antibody was given intravenously on day 1, the mass of aortic valvular vegetation was significantly reduced at day 3; infusion of non-specific rabbit IgG showed no effect. The 99mTc-labeled anti-fibrin antibody also labeled kidneys that showed macroscopic subcapsular hemorrhage. To learn if the deposition of fibrin in the kidneys was a consequence of endocarditis required a comparison of farm-bred and specific pathogen-free rabbits before and after the induction of endocarditis. Before induction, the kidneys of farm-bred rabbits were labeled, but specific pathogen-free rabbits were free of labeling and signs of macroscopic hemorrhage. After 3 days of endocarditis, kidneys of 10 of 14 specific pathogen-free rabbits labeled with 99mTc-labeled anti-fibrin antibody and showed hemorrhage. Kidney lesions were suggested to be a frequent sequellae of S. sanguinis infective endocarditis. For the first time, fibrin was shown to be required for the continued development of aortic valvular vegetations.

  8. Molecular weight fibrinogen variants determine angiogenesis rate in a fibrin matrix in vitro and in vivo

    NARCIS (Netherlands)

    Kaijzel, E.L.; Koolwijk, P.; Erck, M.G.M. van; Hinsbergh, V.W.M. van; Maat, M.P.M. de

    2006-01-01

    Background: During wound repair, fibrin acts both as a barrier to prevent blood loss and as a temporary matrix for the invasion and ingrowth of endothelial and tissue cells. A well-controlled angiogenesis process in the fibrinous exudate matrix is crucial for optimal wound healing. The composition a

  9. Identification of Quantitative Trait Loci for Fibrin Clot Phenotypes. The EuroCLOT Study

    DEFF Research Database (Denmark)

    Williams, Frances M K; Carter, Angela M; Kato, Bernet;

    2009-01-01

    associated with fibrin phenotypes. METHODS AND RESULTS: 447 dizygotic (DZ) and 460 monozygotic (MZ) pairs of healthy UK white female twins and 199 DZ twin pairs from Denmark were studied. D-dimer, an indicator of fibrin turnover, was measured by ELISA and measures of clot formation, morphology, and lysis...

  10. Improvement of fibrin clot structure after factor VIII injection in haemophilia A patients treated on demand.

    Science.gov (United States)

    Antovic, Aleksandra; Mikovic, Danijela; Elezovic, Ivo; Zabczyk, Michael; Hutenby, Kjell; Antovic, Jovan P

    2014-04-01

    Patients with haemophilia A have seriously impaired thrombin generation due to an inherited deficiency of factor (F)VIII, making them form unstable fibrin clots that are unable to maintain haemostasis. Data on fibrin structure in haemophilia patients remain limited. Fibrin permeability, assessed by a flow measurement technique, was investigated in plasma from 20 patients with severe haemophilia A treated on demand, before and 30 minutes after FVIII injection. The results were correlated with concentrations of fibrinogen, FVIII and thrombin-activatable fibrinolysis inhibitor (TAFI), and global haemostatic markers: endogenous thrombin potential (ETP) and overall haemostatic potential (OHP). Fibrin structure was visualised using scanning electron microscopy (SEM). The permeability coefficient Ks decreased significantly after FVIII treatment. Ks correlated significantly with FVIII levels and dosage, and with ETP, OHP and levels of TAFI. SEM images revealed irregular, porous fibrin clots composed of thick and short fibers before FVIII treatment. The clots had recovered after FVIII replacement almost to levels in control samples, revealing compact fibrin with smaller intrinsic pores. To the best of our knowledge, this is the first description of fibrin porosity and structure before and after FVIII treatment of selected haemophilia patients. It seems that thrombin generation is the main determinant of fibrin structure in haemophilic plasma.

  11. Influence of a constant magnetic field on the fibrinogen-fibrin system. [in blood coagulation process

    Science.gov (United States)

    Matskevichene, V. B.; Platonova, A. T.

    1974-01-01

    The effect of a constant magnetic field with a strength of 2500 oersteds on the fibrinogen-fibrin system was studied in the organism of healthy rabbits with exposure times of 1 and 5 hours. The results obtained indicate disruptions in the stage of conversion of fibrinogen to fibrin and an increase in the amount of fibrinogen.

  12. Modification of fibrin network ultrastructure by Fab fragments specific for different domain of fibrinogen.

    Science.gov (United States)

    Cierniewski, C S; Janiak, A; Wyroba, E

    1986-01-01

    Kinetics of inhibition of fibrin monomer polymerization produced by Fab fragments prepared from immunochemically purified monospecific antibodies to the surface epitopes of different domains of fibrinogen molecule has been correlated with electron microscopic observations of resulting specimens. Fab fragments prepared from anti FgD antisera were the most efficient inhibitors of thrombin-catalysed conversion of fibrinogen to fibrin; polymerization of fibrin monomers as detected spectrophotometrically was abolished at 2:1 molar ratio of anti FgD Fab fragments to fibra monomer. These Fab fragments acting as a steric hindrance of polymerization sites inhibited the first stage of fibrin monomer aggregation. Interaction of Fab fragments derived from antibodies specific for alpha 239-476 with corresponding segment of fibrinogen molecule resulted in a weak inhibition of fibrin monomer polymerization. However, fibrin obtained in the presence of these Fab fragments was significantly modified and showed no periodicity. This observation may suggest that anti alpha 239-476 Fab impaired the course of the second stage of fibrin monomer polymerization, i.e. lateral association of fibrin fibrils.

  13. Genetic predictors of fibrin D-dimer levels in healthy adults

    NARCIS (Netherlands)

    N.L. Smith (Nicholas); J.E. Huffman (Jennifer E.); D.P. Strachan (David); J. Huang (Jian); A. Dehghan (Abbas); S. Trompet (Stella); L.M. Lopez (Lorna M.); S.Y. Shin (So Youn); J. Baumert (Jens); V. Vitart (Veronique); J.C. Bis (Joshua); S.H. Wild (Sarah); A. Rumley (Ann); Q. Yang (Qiong Fang); A.G. Uitterlinden (André); D.J. Stott (David. J.); G. Davies (Gareth); A.M. Carter (Angela M.); B. Thorand (Barbara); O. Polašek (Ozren); B. McKnight (Barbara); H. Campbell (Harry); A.R. Rudnicka (Alicja); M.H. Chen (Min-hsin); B.M. Buckley (Brendan M.); S.E. Harris (Sarah); A. Peters (Annette); D. Pulanic (Drazen); T. Lumley (Thomas); A.J.M. de Craen (Anton J.M.); D.C. Liewald (David C.); C. Gieger (Christian); I. Ford (Ian); A.J. Gow (Alan J.); M. Luciano (Michelle); D.J. Porteous (David J.); X. Guo (Xiuqing); N. Sattar (Naveed); A. Tenesa (Albert); M. Cushman (Mary Ann); P.E. Slagboom (Eline); P.M. Visscher (Peter M.); T.D. Spector (Tim); T. Illig (Thomas); I. Rudan (Igor); E.G. Bovill (Edwin G.); A.F. Wright (Alan); W.L. McArdle (Wendy); G.H. Tofler (Geoffrey); A. Hofman (Albert); R.G.J. Westendorp (Rudi); J.M. Starr (John); P.J. Grant (Peter J.); M. Karakas (Mahir); N.D. Hastie (Nicholas D.); B.M. Psaty (Bruce); J.F. Wilson (James); G.D.O. Lowe (Gordon); C.J. O'Donnell (Christopher); J.C.M. Witteman (Jacqueline); J.W. Jukema (Jan Wouter); I.J. Deary (Ian); N. Soranzo (Nicole); W. Koenig (Wolfgang); C. Hayward (Caroline)

    2011-01-01

    textabstractBACKGROUND: Fibrin fragment D-dimer, one of several peptides produced when crosslinked fibrin is degraded by plasmin, is the most widely used clinical marker of activated blood coagulation. To identity genetic loci influencing D-dimer levels, we performed the first large-scale, genome-wi

  14. Methods and preliminary findings of a cost-effectiveness study of glass-ionomer-based and composite resin sealant materials after 2 yr

    NARCIS (Netherlands)

    Goldman, A.S.; Chen, X.; Fan, M.; Frencken, J.E.F.M.

    2014-01-01

    The cost-effectiveness of glass-carbomer, conventional high-viscosity glass-ionomer cement (HVGIC) [without or with heat (light-emitting diode (LED) thermocuring) application], and composite resin sealants were compared after 2 yr in function. Estimated net costs per sealant were obtained from data

  15. Preparation of two component Fibrin Glue and its clinical evaluation in skin grafts and flaps

    Directory of Open Access Journals (Sweden)

    Jain P

    2003-01-01

    Full Text Available Tissue adhesive is one of the alternative to conventional suturing and has some added advantages. Fibrin glue has been used in obtaining haemostasis following trauma to spleen and liver. It has also been used in repair of dural tear and bronchial fistula. Fibrin glue is a biological tissue adhesive based on the final stage of coagulation wherein. Thrombin acting on fibrinogen converts it into fibrin. Thus, it has two components, one is fibrinogen and another is thrombin. We have prepared both components of fibrin glue. Fibrinogen was obtained from patient's own blood and thrombin from fresh frozen plasma of screened healthy donor. The glue was used in 20 cases requiring skin graft or flap. The results were compared with conventional suturing method. Use of the fibrin glue is simple, safe, cost effective, and rapid technique to fix the skin grafts and flaps with avoidance of peroperative bleeding and postoperative collection. It also has better overall results.

  16. Platelet-rich fibrin application in dentistry: a literature review.

    Science.gov (United States)

    Borie, Eduardo; Oliví, Daniel García; Orsi, Iara Augusta; Garlet, Katia; Weber, Benjamín; Beltrán, Víctor; Fuentes, Ramón

    2015-01-01

    The development of bioactive surgical additives to regulate the inflammation and increase the speed of healing process is one of the great challenges in clinical research. In this sense, platelet rich fibrin (PRF) appears as a natural and satisfactory alternative with favorable results and low risks. The following review attempts to summarize the relevant literature regarding the technique of using PRF, focusing on its preparation, advantages, and disadvantages of using it in clinical applications. PRF alone or in combination with other biomaterials seems to have several advantages and indications both for medicine and dentistry, due it is a minimally invasive technique with low risks and satisfactory clinical results.

  17. Fibrin Glue Does Not Improve Healing of Gastrointestinal Anastomoses

    DEFF Research Database (Denmark)

    Nordentoft, Tyge; Pommergaard, Hans-Christian; Rosenberg, Jacob

    2015-01-01

    BACKGROUND/AIM: Anastomotic leakage remains a frequent and serious complication in gastrointestinal surgery. In order to reduce its incidence, several clinical and experimental studies on anastomotic sealing have been performed. In a number of these studies, the sealing material has been fibrin...... on the healing effects of FG on gastrointestinal anastomoses. METHODS: PubMed, EMBASE and the Cochrane databases were searched for studies evaluating the healing process of gastrointestinal anastomoses after any kind of FG application. The search period was from 1953 to December 2013. RESULTS: Twenty...

  18. Microbiology and pathology of fibrinous pericarditis in Danish slaughter pigs

    DEFF Research Database (Denmark)

    Buttenschøn, J.; Friis, N.F.; Aalbaek, B.

    1997-01-01

    The prevalence of fibrinous pericarditis detected at slaughter in Danish slaughter pigs is approximately 0.02 %. The microbiology and pathology of this disorder was studied through 46 field cases collected at slaughter from May 1994 to August 1995. Mycoplasmas (Mycoplasma hyopneumoniae, M...... from the pericardium in three cases. The pericardium was sterile in three cases and contained a low grade mixed flora in two; the latter is believed to be a result of the slaughter procedure rather than an infection. No chlamydiae were found in connection with the microbiological examination...

  19. Revitalization of traumatized immature tooth with platelet-rich fibrin

    Directory of Open Access Journals (Sweden)

    Umrana Faizuddin

    2015-01-01

    Full Text Available Endodontic treatment options for immature, nonvital teeth conventionally include surgical endodontics, apexification with calcium hydroxide, or single visit mineral trioxide aggregate plug. Regeneration is a new concept which is been introduced in the treatment of traumatized open apex tooth. Regeneration of pulp-dentin complex in an infected necrotic tooth with an open apex is possible if the canal is effectively disinfected. The purpose of this case report is to add a new vista in regenerative, endodontic therapy by using platelet-rich fibrin for revitalization of immature nonvital tooth.

  20. Computational imaging analysis of fibrin matrices with the inclusion of erythrocytes from homozygous SS blood reveals agglomerated and amorphous structures.

    Science.gov (United States)

    Averett, Rodney D; Norton, David G; Fan, Natalie K; Platt, Manu O

    2017-01-01

    Sickle cell disease is a single point mutation disease that is known to alter the coagulation system, leading to hypercoagulable plasma conditions. These hypercoagulable conditions can lead to complications in the vasculature, caused by fibrin clots that form undesirably. There is a need to understand the morphology and structure of fibrin clots from patients with sickle cell disease, as this could lead to further discovery of treatments and life-saving therapies. In this work, a computational imaging analysis method is presented to evaluate fibrin agglomeration in the presence of erythrocytes (RBCs) homozygous for the sickle cell mutation (SS). Numerical algorithms were used to determine agglomeration of fibrin fibers within a matrix with SS RBCs to test the hypothesis that fibrin matrices with the inclusion of SS RBCs possess a more agglomerated structure than native fibrin matrices with AA RBCs. The numerical results showed that fibrin structures with SS RBCs displayed an overall higher degree of agglomeration as compared to native fibrin structures. The computational algorithm was also used to evaluate fibrin fiber overlap (aggregation) and anisotropy (orientation) in normal fibrin matrices compared to fibrin matrices polymerized around SS RBCs; however, there was no statistical difference. Ultrasound measurements of stiffness revealed rigid RBCs in the case of samples derived from homozygous SS blood, and densely evolving matrices, when compared to normal fibrin with the inclusion of AA RBCs. An agglomeration model is suggested to quantify the fibrin aggregation/clustering near RBCs for both normal fibrin matrices and for the altered structures. The results of this work are important in the sense that the understanding of aggregation and morphology in fibrin clots with incorporation of RBCs from persons living with sickle cell anemia may elucidate the complexities of comorbidities and other disease complications.

  1. Evaluation of retention of pit and fissure sealants placed with and without air abrasion pretreatment in 6-8 year old children – An in vivo study

    Science.gov (United States)

    Goswami, Mridula

    2017-01-01

    Background The success of pit and fissure sealants is directly related to their retention. The purpose of this study was to assess and compare the retention of pit and fissure sealants placed using acid etch alone and a combination of air abrasion and acid etch techniques. Material and Methods 50 subjects aged 6-8 years were included in the study. Primary second molars and permanent first molars were sealed in all four quadrants using split mouth design. The right maxillary and mandibular molars (Group A) were treated by acid etching alone while the left maxillary and mandibular molars (Group B) were pretreated with air abrasion followed by acid etching before application of pit and fissure sealant. Retention of sealants was checked using Simonsen’s criteria of sealant retention after three and six months of sealant application. Results There was no significant difference in retention of sealants in Group A and Group B (p0.05). Maxillary molars showed superior retention compared to mandibular molars, which was statistically significant at both three and six months (p<0.05). Conclusions Combining air abrasion pretreatment with subsequent acid etching did not result in statistically significant difference in sealant retention compared to acid etching alone in both primary and permanent molars after 3 and 6 months follow up. An additional air abrasion pretreatment step can be avoided in pediatric patients when placing sealants and the procedure can be completed faster with better behavior management using acid etching alone. Key words:Pit and fissure sealant, acid etching, air abrasion. PMID:28210438

  2. Fibrin glue in the treatment of anal fistula: a systematic review

    Directory of Open Access Journals (Sweden)

    Covarelli Piero

    2009-11-01

    Full Text Available Abstract Background New sphincter-saving approaches have been applied in the treatment of perianal fistula in order to avoid the risk of fecal incontinence. Among them, the fibrin glue technique is popular because of its simplicity and repeatability. The aim of this review is to compare the fibrin glue application to surgery alone, considering the healing and complication rates. Methods We performed a systematic review searching for published randomized and controlled clinical trials without any language restriction by using electronic databases. All these studies were assessed as to whether they compared conventional surgical treatment versus fibrin glue treatment in patients with anal fistulas, in order to establish both the efficacy and safety of each treatment. We used Review Manager 5 to conduct the review. Results The healing rate is higher in those patients who underwent the conventional surgical treatment (P = 0,68, although the treatment with fibrin glue gives no evidence of anal incontinence (P = 0,08. Furthermore two subgroup analyses were performed: fibrin glue in combination with intra-adhesive antibiotics versus fibrin glue alone and anal fistula plug versus fibrin glue. In the first subgroup there were not differences in healing (P = 0,65. Whereas in the second subgroup analysis the healing rate is statistically significant for the patients who underwent the anal fistula plug treatment instead of the fibrin glue treatment (P = 0,02. Conclusion In literature there are only two randomized controlled trials comparing the conventional surgical management versus the fibrin glue treatment in patients with anal fistulas. Although from our statistical analysis we cannot find any statistically significant result, the healing rate remains higher in patients who underwent the conventional surgical treatment (P = 0,68, and the anal incontinence rate is very low in the fibrin glue treatment group (P = 0,08. Anyway the limited collected data do

  3. Collector sealants and breathing. Mid-term report, September 25, 1978-May 30, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Mendelsohn, M. A.; Yeoman, F. A.; Luck, R. M.; Navish, Jr, F. W.; Meier, J. F.

    1978-01-01

    The objectives of this program are: (1) to investigate the pertinent properties of a variety of possible sealants for solar collectors and identify the most promising candidates and to study the effect of breathing in flat-plate, thermal solar collector units. The study involves two types of sealants, Class PS which includes performed seals or gaskets and Class SC which includes sealing compounds or caulks. It is the intent of the study to obtain data regarding initial properties of candidate elastomers from manufacturers and from the technical literature and to use those sources to provide data pertaining to endurance of these materials under environmental service conditions. Where necessary, these data are being augmented by experimental measurements. Environmental stresses to be evaluated by these measuremets include elevated temperatures, moisture, ultraviolet light, ozone and oxygen, and fungus. The second major area of the work involves a study of the effects of materials used and design on the durability of solar collectors. Factors such as design, fabrication, materials of construction, seals and sealing techniques and absorber plate coatings were observed on actual field units removed from service. Such phenomena as leakage, corrosion and formation of deposits on glazing and absorber plate were noted. An evaluation of the properties of several desiccants is being made in order to provide means to mitigate the deleterious effects of water on collector life.Absorbents for organic degradation products of sealants are also being investigated in order to protect the glazing and absorber plate from deposited coatings. Since adsorbents and desiccants in general tend to take up both waer and organic decomposition products, relative affinities of a number of these agents for water and for organic compounds are being determined.

  4. Collector sealants and breathing. Final Report, 25 September 1978-31 December 1979

    Energy Technology Data Exchange (ETDEWEB)

    Mendelsohn, M A; Luck, R M; Yeoman, F A; Navish, Jr, F W

    1980-02-20

    The objectives of this program were: (1) to investigate the pertinent properties of a variety of possible sealants for solar collectors and identify the most promising candidates, and (2) to study the effect of breathing in flat-plate, thermal solar collector units. The study involved two types of sealants, Class PS which includes preformed seals or gaskets and Class SC which includes sealing compounds or caulks. It was the intent of the study to obtain data regarding initial properties of candidate elastomers from manufacturers and from the technical literature and to use those sources to provide data pertaining to endurance of these materials under environmental service conditions. Where necessary, these data were augmented by experimental measurements. Environmental stresses evaluated by these measurements included elevated temperatures, moisture, ultraviolet light, ozone and oxygen, and fungus. The second major area of the work involved a study of the effects of materials used and design on the durability of solar collectors. Factors such as design, fabrication, materials of construction, seals and sealing techniques and absorber plate coatings were observed on actual field units removed from service. Such phenomena as leakage, corrosion and formation of deposits on glazing and absorber plate were noted. An evaluation of the properties of several desiccants was made in order to providemeans to mitigate the deleterious effects of water on collector life. Adsorbents for organic degradation products of sealants were also investigated in order to protect the glazing and absorber plate from deposited coatings. Since adsorbents and desiccants in general tend to take up both water and organic decomposition products, relative affinities of a number of these agents for water and for organic compounds were determined . Results are presented in detail.

  5. The effect of different enamel surface treatments on microleakage of fissure sealants

    Directory of Open Access Journals (Sweden)

    Asli Topaloglu-Ak

    2013-11-01

    Full Text Available Objective. The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of fissure sealants in permanent molar teeth in vitro. Materials and methods. 96 extracted impacted human third molars were randomly divided into 8 surface treatment groups (n=12/group as 1. Er: YAG laser; (Fidelis II, Fotona, Ljubljana, Slovenia (125 mj, 20Hz. 2. Er: YAG laser + 37% H3PO4 (15s; 3. ER: AG laser + 37% H3PO4+Prime&Bond NT; 4. Er: YAG laser + G Bond; 5. Er: YAG laser + Prime&Bond NT; 6. 37% H3PO4; 7. 37% H3PO4 + Prime &Bond NT; 8. G Bond. Sealant material (Clinpro, 3M ESPE, Seefeld, Germany, was applied into the fissures and light-cured for 20s with LED (Bluephase C5, Ivoclar-Vivadent, Schaan, Liechtenstein. Specimens were subjected to thermocycling (1000×, 5-55°C, dwell time: 15s and immersed in 0.5% basic fuchsin solution for 24h at 37°C. The samples were sectioned and scored on a 3 point rating scale using a light microscope with a magnification of ×20. One-way analysis of variance was used to analyze data. Multiple comparisons were analyzed using Bonferroni test (p=0.05. Results. Er:YAG laser showed the highest microleakage scores whereas Er YAG laser + 37% H3PO4 showed the lowest. Although 37% H3PO4 group showed higher scores than Er:YAG laser + 37% H3PO4, the difference was not statistically significant. Conclusion. Etching fissures with phosphoric acid is sufficient prior to fissure sealant application.

  6. Soft fibrin gels promote selection and growth of tumorigenic cells

    Science.gov (United States)

    Liu, Jing; Tan, Youhua; Zhang, Huafeng; Zhang, Yi; Xu, Pingwei; Chen, Junwei; Poh, Yeh-Chuin; Tang, Ke; Wang, Ning; Huang, Bo

    2012-08-01

    The identification of stem-cell-like cancer cells through conventional methods that depend on stem cell markers is often unreliable. We developed a mechanical method for selecting tumorigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness. When cultured within these gels, primary human cancer cells or single cancer cells from mouse or human cancer cell lines grew within a few days into individual round colonies that resembled embryonic stem cell colonies. Subcutaneous or intravenous injection of 10 or 100 fibrin-cultured cells in syngeneic or severe combined immunodeficiency mice led to the formation of solid tumours at the site of injection or at the distant lung organ much more efficiently than control cancer cells selected using conventional surface marker methods or cultured on conventional rigid dishes or on soft gels. Remarkably, as few as ten such cells were able to survive and form tumours in the lungs of wild-type non-syngeneic mice.

  7. Fibrin formation and dissolution in women receiving oral contraceptive drugs.

    Science.gov (United States)

    Ball, A P; McKee, P A

    1977-04-01

    Factors affecting fibrin formation and dissolution were compared for 15 women taking combined oral contraceptives and 15 women using nonpharmacological methods of birth control. The two groups were matched for age, body weight, time of blood collection, and day in menstrual cycle; none of the women was receiving other drugs known to affect the blood coagulation or fibrinolytic parameters measured in this study. Fibrinogen concentrations tended to be higher in the experimental group; the degree of fibrinogen degradation, number of fibrin cross-links, and levels of factor XIII and plasminogen were the same for both group. There were significant reductions in antithrombin activity, the euglobulin lysis time, and fibrinolytic inhibitor level in women using oral contraceptives. An estrogen dose effect was suggested for fibrinogen concentration and the degree of antithrombin activity. The increased fibrinolytic activity and decreased fibrinolytic inhibitor levels are consistent with in vitro observations that antithrombin also inhibits plasmin activity. Thus while oral contraceptive-induced depression of antithrombin III could possibly predispose to thrombosis by diminishing the inhibition of the serine protease clotting factors, the concomitant decreased level of plasmin inhibition might balance the system by favoring thrombolysis as well as the digestion and inactivation of certain clotting factors by plasmin.

  8. Teknik Lem Fibrin Otologus pada Cangkok Konjungtiva Bulbi Mata Kelinci

    Directory of Open Access Journals (Sweden)

    Sutarya Enus

    2009-12-01

    Full Text Available Commercial Fibrin Glue (CFG as a biomaterial adhesive from fresh frozen plasma donor and bovine has been tested having various superiorities compared to suturing in bulbar conjunctiva grafting of pterygium surgery. Due to CFG has not been available in Indonesia led the author to make an Autologous Fibrin Glue (AFG directly from patient's own blood. The study aim was to investigate the effectiveness of AFG attachment. This study was an animal experimental, conducted in 24 New Zealand White rabbits divided into AFG group and suturing group of 12 rabbits each, held in Laboratory of Bio Farma, Bandung, from September to December 2006. The assessment included duration (in minute of AFG compared to suturing techniques, also at first day and one week post grafting attachments in bulbar conjunctiva grafting. The statistical analysis used Mann-Whitney and Wilcoxon rank test. In time duration of surgical treatment, AFG technique was significantly shorter than suturing technique (p=0.0001, while first day and one week post grafting AFG attachment showed significantly greater than those of suturing attachment (p=0.0001. In the meantime, first day and one week post grafting AFG attachment was steady state, whereas in suturing technique was different (p=0.0174. In conclusion, AFG technique is more effective than suturing technique in bulbar conjunctiva grafting because of shorter surgical duration, excellent and stable graft attachment.

  9. Fibrin/platelet plug counteracts cutaneous wound contraction: the hypothesis of "skipping stone".

    Science.gov (United States)

    Farahani, Ramin Mostofi Zadeh

    2007-01-01

    Cutaneous wound contraction and epithelialization act collaboratively to minimize the exposed wound surface. However excessive wound contraction is undesirable due to the resultant disfigurement and scarring. Fibrin clot has greater stiffness than surrounding tissue and mechanical strain further enhances its stiffness. On the contrary, skin exhibits diminished stiffness when affected by high strain rates. Therefore during early stages of wound healing, the contractile wound border is confronted by fibrin clot forming a high strain region in the interface of contractile tissue and fibrin clot--which is evidenced by computer simulation. Due to the stress relaxation property of skin, the contractile strain is partly neutralized. Meanwhile, gradually the stiffness of fibrin clot decreases which is followed by another cycle of wound contraction. This cyclic pattern of contraction resembles the movement of a stone over water or "skipping stone". The stone bounces repeatedly when thrown across the surface of water with reduction of jumping altitude with each bounce till the stone stops completely. This hypothesis is further supported by the observed initial delay in wound contraction and the chronological correlation of enhanced wound contraction with loss of superficial eschar and substitution of fibrin clot with granulation tissue. Also there is evidence that fibrin inhibits fibroblast-mediated contraction of collagen. Furthermore, modest increase in wound contraction rate in fibrinogen deficient mice and fibrin-mediated diminished wound contraction are agreement with the proposed hypothesis.

  10. Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix.

    Science.gov (United States)

    Greiling, D; Clark, R A

    1997-04-01

    After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into the fibrin clot and contribute to the formation of granulation tissue. The functional mechanisms allowing fibroblasts to leave the collagenous matrix of normal connective tissue and invade the provisional matrix of the fibrin clot have not been fully defined. To investigate these mechanisms we established a new in vitro model which simulates specific aspects of early wound healing, that is, the migration of fibroblasts from a three-dimensional collagen matrix into a fibrin clot. This transmigration could be induced by physiological concentrations of platelet releasate or platelet-derived growth factor BB (PDGF-BB) in a concentration-dependent manner. At 24 hours irradiated fibroblasts invaded the fibrin gel almost as well as non-irradiated cells, indicating that transmigration was independent of proliferation. Plasminogen and its activators appear to be necessary for invasion of the fibrin clot since protease inhibitors decreased the amount of migration. These serine proteases, however, were not necessary for exit from the collagen gel as fibroblasts migrated out of the collagen gel onto a surface coated with fibrin fibrils even in the presence of inhibitors. Removal of fibronectin (FN) from either the collagen gel or the fibrin gel markedly decreased the number of migrating cells, suggesting that FN provides a conduit for transmigration. Cell movement in the in vitro model was inhibited by RGD peptide, and by monoclonal antibodies against the subunits of the alpha5 beta1 and alpha v beta3 integrin receptor. Thus, the functional requirements for fibroblast transmigration from collagen-rich to fibrin-rich matrices, such as occurs in early wound healing, have been partially defined using an in vitro paradigm of this important biologic process.

  11. The Platelet Integrin αIIbβ3 Differentially Interacts with Fibrin Versus Fibrinogen.

    Science.gov (United States)

    Litvinov, Rustem I; Farrell, David H; Weisel, John W; Bennett, Joel S

    2016-04-08

    Fibrinogen binding to the integrin αIIbβ3 mediates platelet aggregation and spreading on fibrinogen-coated surfaces. However,in vivoαIIbβ3 activation and fibrinogen conversion to fibrin occur simultaneously, although the relative contributions of fibrinogenversusfibrin to αIIbβ3-mediated platelet functions are unknown. Here, we compared the interaction of αIIbβ3 with fibrin and fibrinogen to explore their differential effects. A microscopic bead coated with fibrinogen or monomeric fibrin produced by treating the immobilized fibrinogen with thrombin was captured by a laser beam and repeatedly brought into contact with surface-attached purified αIIbβ3. When αIIbβ3-ligand complexes were detected, the rupture forces were measured and displayed as force histograms. Monomeric fibrin displayed a higher probability of interacting with αIIbβ3 and a greater binding strength. αIIbβ3-fibrin interactions were also less sensitive to inhibition by abciximab and eptifibatide. Both fibrinogen- and fibrin-αIIbβ3 interactions were partially inhibited by RGD peptides, suggesting the existence of common RGD-containing binding motifs. This assumption was supported using the fibrin variants αD97E or αD574E with mutated RGD motifs. Fibrin made from a fibrinogen γ'/γ' variant lacking the γC αIIbβ3-binding motif was more reactive with αIIbβ3 than the parent fibrinogen. These results demonstrate that fibrin is more reactive with αIIbβ3 than fibrinogen. Fibrin is also less sensitive to αIIbβ3 inhibitors, suggesting that fibrin and fibrinogen have distinct binding requirements. In particular, the maintenance of αIIbβ3 binding activity in the absence of the γC-dodecapeptide and the α-chain RGD sequences suggests that the αIIbβ3-binding sites in fibrin are not confined to its known γ-chain and RGD motifs.

  12. Morphological Study of Isolated Ovarian Preantral Follicles Using Fibrin Gel Plus Platelet Lysate after Subcutaneous Transplantation

    Directory of Open Access Journals (Sweden)

    Ali Reza Rajabzadeh

    2015-04-01

    Full Text Available Objective: Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation. Materials and Methods: In this experimental study, ovaries were taken from 14-dayold Naval Medical Research Institute (NMRI mice. Preantral follicles were dissected from the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20% PL, then transplanted back into the same donor mice. Fibrin gels supplemented with PL that contained preantral follicles were placed in a subcutaneous pocket in the back of the neck of the recipient, donor mouse (the same mouse that follicles were collected. After 14 days the grafts were processed and embedded in paraffin blocks, then serially sectioned for histological evaluation. We counted the follicles and classified them according to stage (preantral or antral. Data were presented as mean ± standard error of mean (SEM and analysed by analysis of variance (ANOVA and the Kruskal-Wallistest. Results: The mean percentage of recovered follicles encapsulated and transplanted in each group were 33.30 ± 2.47 (fibrin gel, 31.96 ± 1.90 (fibrin gel+5% PL, 34.02 ± 2.44 (fibrin gel+10% PL, 48.31 ± 2.06 (fibrin gel+15% PL and 17.60 ± 2.79 (fibrin gel+20% PL. There was a significant increase in the recovery rate of grafted follicles with fibrin gel+15% PL (48.31%; p<0.001. The percentage of preantral follicles showed no significant difference in all groups (p<0.05. The percentage of antral follicles showed a significant decrease in follicles grafted with fibrin gel+20% PL when compared to the other groups (11.77%; p<0.005 but no significant difference was observed in the other groups. Conclusion: The use of PL in follicle

  13. Radon penetration of concrete slab cracks, joints, pipe penetrations, and sealants.

    Science.gov (United States)

    Nielson, K K; Rogers, V C; Holt, R B; Pugh, T D; Grondzik, W A; de Meijer, R J

    1997-10-01

    Radon movement through 12 test slabs with different cracks, pipe penetrations, cold joints, masonry blocks, sealants, and tensile stresses characterized the importance of these anomalous structural domains. Diffusive and advective radon transport were measured with steady-state air pressure differences controlled throughout the deltaP = 0 to 60 Pa range. Diffusion coefficients (deltaP = 0) initially averaged 6.5 x 10(-8) m2 s(-1) among nine slabs with only 8% standard deviation, but increased due to drying by 0.16% per day over a 2-y period to an average of 2.0 x 10(-7) m2 s(-1). An asphalt coating reduced diffusion sixfold but an acrylic surface sealant had no effect. Diffusion was 42 times higher in solid masonry blocks than in concrete and was not affected by small cracks. Advective transport (deltaP permeability), pipe penetrations, and caulked gaps, but was significant for cracks, disturbed pipe penetrations, cold joints, masonry blocks, and concrete under tensile stress. Crack areas calculated to be as small as 10(-7) m2 significantly increased radon advection. Algebraic expressions predict air velocity and effective crack width from enhanced radon transport and air pressures. Masonry blocks, open cracks, and slab cold joints enhance radon penetration but stressed slabs, undisturbed pipe penetrations, and sealed cracks may not.

  14. Evaluation of the effects of enameloplasty and air abrasion on sealant micro-leakage.

    Directory of Open Access Journals (Sweden)

    Aliasghar Soleymani

    2014-12-01

    Full Text Available Micro-leakage, one of the major reasons for recurrent decays, may lead to uncontrollable flow of liquids, pulp inflammation and peri-apical pathology. The purpose of this in vitro study was to evaluate micro-leakage of pit and fissure sealants after using three different pit and fissure preparation techniques: 1 acid etching, 2 fissure enameloplasty and acid etching, and 3 air abrasion and acid etching.Sixty-nine extracted sound molars and premolars were randomly divided into three groups (23 samples in each group. Teeth were prepared using one of three occlusal surface treatments. Then the sealant was applied on the occlusal fissures of all teeth. The teeth were thermocycled and stored in normal saline. All teeth were sealed apically and coated with nail varnish and then were immersed in a 2% solution of methylen blue. Two buccolingual sections were made. The surfaces were scored 0 to 3 for the extent of micro-leakage using a stereomicroscope. Data were analyzed by Kruskal- wallis and Dunn procedure.Enameloplasty and acid etching were significantly different regarding micro-leakage (P= 0.016, but no significant difference in micro-leakage was observed between air abrasion and the other methods.Enameloplasty followed by acid etching produced significantly less micro-leakage than the acid etching technique. Air abrasion acid etching was better than the acid etching technique in reducing micro-leakage too.

  15. Creep Behavior of Glass/Ceramic Sealant and its Effect on Long-term Performance of Solid Oxide Fuel Cells

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Wenning N.; Sun, Xin; Koeppel, Brian J.; Stephens, Elizabeth V.; Khaleel, Mohammad A.

    2009-10-14

    The creep behavior of glass or glass-ceramic sealant materials used in solid oxide fuel cells (SOFCs) becomes relevant under SOFC operating temperatures. In this paper, the creep of glass-ceramic sealants was experimentally examined, and a standard linear solid model was applied to capture the creep behavior of glass ceramic sealant materials developed for planar SOFCs at high temperatures. The parameters of this model were determined based on the creep test results. Furthermore, the creep model was incorporated into finite-element software programs SOFC-MP and Mentat-FC developed at Pacific Northwest National Laboratory for multi-physics simulation of SOFCs. The effect of creep of glass ceramic sealant materials on the long-term performance of SOFC stacks was investigated by studying the stability of the flow channels and the stress redistribution in the glass seal and on the various interfaces of the glass seal with other layers. Finite element analyses were performed to quantify the stresses in various parts. The stresses in glass seals were released because of creep behavior during operations.

  16. Sealant retention is better assessed through colour photographs than through the replica and the visual examination methods

    NARCIS (Netherlands)

    Hu, X.; Fan, M.; Rong, W.; Lo, E.C.; Bronkhorst, E.M.; Frencken, J.E.F.M.

    2014-01-01

    The aim of this study was to test the hypothesis that the colour photograph method has a higher level of validity for assessing sealant retention than the visual clinical examination and replica methods. Sealed molars were assessed by two evaluators. The scores for the three methods were compared ag

  17. Growth of hydroxyapatite on physiologically clotted fibrin capped gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Sastry, T P; Sundaraseelan, J; Swarnalatha, K; Sobhana, S S Liji; Makheswari, M Uma; Sekar, S; Mandal, A B [Central Leather Research Institute, Chennai-600020, Tamilnadu (India)], E-mail: sastrytp@hotmail.com, E-mail: abmandal@hotmail.com

    2008-06-18

    The growth of hydroxyapatite (HAp) on physiologically clotted fibrin (PCF)-gold nanoparticles is presented for the first time by employing a wet precipitation method. Fourier transform infrared (FTIR) spectroscopy confirmed the characteristic functionalities of PCF and HAp in the PCF-Au-HAp nanocomposite. Scanning electron microscopy (SEM) images have shown cuboidal nanostructures having a size in the range of 70-300 nm of HAp, whereas 2-50 nm sized particles were visualized in high-resolution transmission electron microscopy (TEM). Energy-dispersive x-ray (EDX) and x-ray diffraction (XRD) studies have confirmed the presence of HAp. These results show that gold nanoparticles with PCF acted as a matrix for the growth of HAp, and that PCF-Au-HAp nanocomposite is expected to have better osteoinductive properties.

  18. Fibrin powder turbidity measurement for rapid assessment of antiplasmins.

    Science.gov (United States)

    Exner, T; Rickard, K A; Kronenberg, H

    1975-11-01

    Fibrinolytic activity was determined from the rate of disappearance of turbidity in a suspension of heat-treated fibrin powder. Using this method for estimating residual fibrinolytic activity in mixtures of serum and plasmin, antiplasmin behaviors of specimens from patients with various clinical disorders were determined after long and short preincubation times. Slow-acting antiplasmins were found to be increased in a variety of conditions among these patients, while immediate acting antiplasmins were generally decreased, compared with those in specimens from a large pool of normal, healthy vounteers. Normal women taking oral contraceptives had consitently high levels of slow antiplasmins. Tests in vitro showed that the antifibrinolytic agents epsilon-aminocaproic acid, Trasylol and soybean trypsin inhibitor act only as fast antiplasmins.

  19. Platelet-rich-fibrin: A novel root coverage approach

    Directory of Open Access Journals (Sweden)

    Anilkumar K

    2009-01-01

    Full Text Available Treatment of gingival recession has become an important therapeutic issue due to increasing cosmetic demand. Multiple surgical procedures have been developed to obtain predictable esthetic root coverage. More specifically, after periodontal regenerative surgery, the aim is to achieve complete wound healing and regeneration of the periodontal unit. A recent innovation in dentistry is the preparation and use of platelet-rich plasma (PRP, a concentrated suspension of the growth factors, found in platelets. These growth factors are involved in wound healing and postulated as promoters of tissue regeneration. This paper reports the use of PRF membrane for root coverage on the labial surfaces of the mandibular anterior teeth. This was accomplished using laterally displaced flap technique with platelet rich fibrin (PRF membrane at the recipient site.

  20. A recently developed bifacial platelet-rich fibrin matrix

    Directory of Open Access Journals (Sweden)

    E Lucarelli

    2010-07-01

    Full Text Available Platelet-rich plasma (PRP is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET®, which is a dense pliable, platelet-rich fibrin matrix (PRFM. We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC. Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 + 314.6 kPa, stress at a break of 1476.0 + 526.3 kPa, and an elongation at break of 146.3 + 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-β1 were measured in the day 1-conditioned media (CM of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  1. A recently developed bifacial platelet-rich fibrin matrix.

    Science.gov (United States)

    Lucarelli, E; Beretta, R; Dozza, B; Tazzari, P L; O'Connel, S M; Ricci, F; Pierini, M; Squarzoni, S; Pagliaro, P P; Oprita, E I; Donati, D

    2010-07-01

    Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair. Because of the poor mechanical properties, conventional PRP is often difficult to handle when used in clinical settings and requires secure implantation in a specific site, otherwise when released growth factors could be washed out during an operation. In this study, we analyzed the end product of a recently developed commercially available system (FIBRINET), which is a dense pliable, platelet-rich fibrin matrix (PRFM). We characterized the mechanical properties of PRFM and tested whether PRFM releases growth factors and whether released factors induce the proliferation of mesenchymal stem cells (MSC). Mechanical properties as well as platelet distribution were evaluated in PRFM. PRFM demonstrated robust mechanical properties, with a tear elastic modulus of 937.3 +/- 314.6 kPa, stress at a break of 1476.0 +/- 526.3 kPa, and an elongation at break of 146.3 +/- 33.8 %. PRFM maintained its mechanical properties throughout the testing process. Microscopic observations showed that the platelets were localized on one side of the matrix. Elevated levels of PDGF-AA, PDGF-AB, EGF, VEGF, bFGF and TGF-beta1 were measured in the day 1-conditioned media (CM) of PRFM and growth factor levels decreased thereafter. BMP2 and BMP7 were not detectable. MSC culture media supplemented with 20% PRFM-CM stimulated MSC cell proliferation; at 24 and 48 hours the induction of the proliferation was significantly greater than the induction obtained with media supplemented with 20% foetal bovine serum. The present study shows that the production of a dense, physically robust PRFM made through high-speed centrifugation of intact platelets and fibrin in the absence of exogenous thrombin yields a potential tool for accelerating tissue repair.

  2. Platelet-rich fibrin versus albumin in surgical wound repair: a randomized trial with paired design

    DEFF Research Database (Denmark)

    Danielsen, Patricia L; Ågren, Sven Per Magnus; Jørgensen, Lars Nannestad

    2010-01-01

    To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial....

  3. Pit and Fissure Sealants with Different Materials: Resin Based x Glass Ionomer Cement – Results after Six Months

    Directory of Open Access Journals (Sweden)

    Priscilla Santana Pinto Gonçalves

    2016-01-01

    Full Text Available Objective: To compare the retention and superficial characteristics between a new resin-modified glass ionomer sealant and resin sealant. In addition, the teeth sealed were compared to partially erupted first molars (control group without sealing in relation to the incidence of dental caries. Material and Methods: Initially, 31 children aged 6-8 years participated in this study. The study children showed caries history, but had at least two healthy first molars. A total of 114 teeth were randomly divided into three groups: Clinpro (n=36, Fluroshield (n=38, and control (n=40. The two sealants were applied under relative isolation after previous prophylaxis and enamel etching with phosphoric acid 35% (15s. Clinical evaluation was performed by two calibrated examiners. They evaluated the retention and surface characteristics of the occlusal surface. Results: Fluroshield® showed significantly greater retention than ClinproTM Varnish® XT (p=0.002. However, the performance analysis of the success and failure of retention presented no significant difference between the materials (p=0.141. Concerning to the surface characteristics, Fluroshield performed better than Clinpro after analyzing the marginal deterioration, marginal discoloration, and superficial discoloration (p<0.05. The surface texture of the materials under study was similar (p=0.071. Sealed groups (Clinpro=Fluroshield showed similar performance in the prevention of dental caries, which was significantly lower than that of control group (p=0.001. Conclusion: Both sealants, Fluroshield® and Clinpro TM Varnish® XT were effective in preventing caries lesion within 6 months, although Fluroshield sealant showed better clinical retention.

  4. The effect of surface sealants with different filler content on microleakage of Class V resin composite restorations

    Science.gov (United States)

    Hepdeniz, Ozge Kam; Temel, Ugur Burak; Ugurlu, Muhittin; Koskan, Ozgur

    2016-01-01

    Objective: Microleakage is still one of the most cited reasons for failure of resin composite restorations. Alternative methods to prevent microleakage have been investigated increasingly. The aim of this study is to evaluate the microleakage in Class V resin composite restorations with or without application of surface sealants with different filler content. Materials and Methods: Ninety-six cavities were prepared on the buccal and lingual surfaces with the coronal margins located in enamel and the cervical margins located in dentin. The cavities restored with an adhesive system (Clearfil SE Bond, Kuraray, Tokyo, Japan) and resin composite (Clearfil Majesty ES-2, Kuraray, Tokyo, Japan). Teeth were stored in distilled water for 24 h and separated into four groups according to the surface sealants (Control, Fortify, Fortify Plus, and G-Coat Plus). The teeth were thermocycled (500 cycles, 5–55° C), immersed in basic fuchsine, sectioned, and analyzed for dye penetration using stereomicroscope. The data were submitted to statistical analysis by Kruskal–Wallis and Bonferroni–Dunn test. Results: The results of the study indicated that there was minimum leakage at the enamel margins of all groups. Bonferroni–Dunn tests revealed that Fortify and GC-Coat groups showed significantly less leakage than the Control group and the Fortify Plus group at dentin margins in lingual surfaces (P < 0.05). Conclusion: The all surface sealants used in this study eliminated microleakage at enamel margins. Moreover, unfilled or nanofilled surface sealants were the most effective in decreasing the degree of marginal microleakage at dentin margins. However, viscosity and penetrability of the sealants could be considered for sealing ability besides composition. PMID:27095890

  5. [APPLICATION OF FISTULA PLUG WITH THE FIBRIN ADHESIVE IN TREATMENT OF RECTAL FISTULAS].

    Science.gov (United States)

    Aydinova, P R; Aliyev, E A

    2015-05-01

    Results of surgical treatment of 21 patients, suffering high transsphincteric and extrasphincteric rectal fistulas, were studied. In patients of Group I the fistula passage was closed, using fistula plug obturator; and in patients of Group II--by the same, but preprocessed by fibrin adhesive. The fistula aperture germeticity, prophylaxis of rude cicatrices development in operative wound zone, promotion of better fixation of bioplastic material were guaranteed, using fistula plug obturator with preprocessing, using fibrin adhesive.

  6. Platelet-rich fibrin versus albumin in surgical wound repair: a randomized trial with paired design

    DEFF Research Database (Denmark)

    Danielsen, Patricia L; Ågren, Sven Per Magnus; Jørgensen, Lars Nannestad

    2010-01-01

    To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial.......To study the effects of autologous platelet-rich fibrin (PRF) versus human albumin on incisional wound breaking strength and subcutaneous collagen deposition in patients undergoing laparoscopic cholecystectomy in a randomized trial....

  7. Interpenetrating Fibrin-Alginate Matrices for in vitro Ovarian Follicle Development

    OpenAIRE

    Shikanov, Ariella; Xu, Min; Woodruff, Teresa K.; Shea, Lonnie D.

    2009-01-01

    In this report, we investigate the fibrin-alginate inter penetrating network (FA-IPN) to provide dynamic cell-responsive mechanical properties, which we apply to the in vitro growth of ovarian follicles. The mechanical properties and polymerization rate of the gels were investigated by rheology, and the fiber structure was imaged by electron microscopy. Using a mouse model, two-layered secondary follicles were encapsulated in FA-IPNs, and growth, morphology, hormone production, fibrin degrada...

  8. A neoantigenic determinant in the D-dimer fragment of fibrin

    NARCIS (Netherlands)

    Lugovskoy, E.V.; Kolesnikova, I.N.; Gritsenko, P.G.; Zolotareva, E.N.; Gaffney, P.; Nieuwenhuizen, W.; Komisarenko, S.V

    2002-01-01

    Monoclonal antibody (mAb) III-3b binds D-dimer with Kd = 1.4 × 10-10 M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bβ134-190, presumably in Bβ155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant i

  9. An Antithrombin-Heparin Complex Increases the Anticoagulant Activity of Fibrin Clots

    Directory of Open Access Journals (Sweden)

    Lesley J. Smith

    2008-01-01

    Full Text Available Clotting blood contains fibrin-bound thrombin, which is a major source of procoagulant activity leading to clot extension and further activation of coagulation. When bound to fibrin, thrombin is protected from inhibition by antithrombin (AT + heparin but is neutralized when AT and heparin are covalently linked (ATH. Here, we report the surprising observation that, rather than yielding an inert complex, thrombin-ATH formation converts clots into anticoagulant surfaces that effectively catalyze inhibition of thrombin in the surrounding environment.

  10. Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft

    OpenAIRE

    2012-01-01

    Platelet-rich fibrin has long been used as a wound healing therapy in skin wounds and recently evidence has suggested its usage in oral cavity for different treatment procedures. This article proposes an overview of use of platelet-rich fibrin in management of complicated oral wounds. Excessive hemorrhage of the donor area, necrosis of epithelium, and morbidity associated with donor site have been described as the possible complications after harvesting subepithelial connective tissue graft, ...

  11. Fibrin Gel as an Injectable Biodegradable Scaffold and Cell Carrier for Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Yuting Li

    2015-01-01

    Full Text Available Due to the increasing needs for organ transplantation and a universal shortage of donated tissues, tissue engineering emerges as a useful approach to engineer functional tissues. Although different synthetic materials have been used to fabricate tissue engineering scaffolds, they have many limitations such as the biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate. Fibrin gel, a biopolymeric material, provides numerous advantages over synthetic materials in functioning as a tissue engineering scaffold and a cell carrier. Fibrin gel exhibits excellent biocompatibility, promotes cell attachment, and can degrade in a controllable manner. Additionally, fibrin gel mimics the natural blood-clotting process and self-assembles into a polymer network. The ability for fibrin to cure in situ has been exploited to develop injectable scaffolds for the repair of damaged cardiac and cartilage tissues. Additionally, fibrin gel has been utilized as a cell carrier to protect cells from the forces during the application and cell delivery processes while enhancing the cell viability and tissue regeneration. Here, we review the recent advancement in developing fibrin-based biomaterials for the development of injectable tissue engineering scaffold and cell carriers.

  12. Fibrin Networks Support Recurring Mechanical Loads by Adapting their Structure across Multiple Scales.

    Science.gov (United States)

    Kurniawan, Nicholas A; Vos, Bart E; Biebricher, Andreas; Wuite, Gijs J L; Peterman, Erwin J G; Koenderink, Gijsje H

    2016-09-06

    Tissues and cells sustain recurring mechanical loads that span a wide range of loading amplitudes and timescales as a consequence of exposure to blood flow, muscle activity, and external impact. Both tissues and cells derive their mechanical strength from fibrous protein scaffolds, which typically have a complex hierarchical structure. In this study, we focus on a prototypical hierarchical biomaterial, fibrin, which is one of the most resilient naturally occurring biopolymers and forms the structural scaffold of blood clots. We show how fibrous networks composed of fibrin utilize irreversible changes in their hierarchical structure at different scales to maintain reversible stress stiffening up to large strains. To trace the origin of this paradoxical resilience, we systematically tuned the microstructural parameters of fibrin and used a combination of optical tweezers and fluorescence microscopy to measure the interactions of single fibrin fibers for the first time, to our knowledge. We demonstrate that fibrin networks adapt to moderate strains by remodeling at the network scale through the spontaneous formation of new bonds between fibers, whereas they adapt to high strains by plastic remodeling of the fibers themselves. This multiscale adaptation mechanism endows fibrin gels with the remarkable ability to sustain recurring loads due to shear flows and wound stretching. Our findings therefore reveal a microscopic mechanism by which tissues and cells can balance elastic nonlinearity and plasticity, and thus can provide microstructural insights into cell-driven remodeling of tissues.

  13. Fibrin clot properties and haemostatic function in men and women with type 1 diabetes.

    Science.gov (United States)

    Tehrani, Sara; Jörneskog, Gun; Ågren, Anna; Lins, Per-Eric; Wallén, Håkan; Antovic, Aleksandra

    2015-02-01

    The increased risk of vascular complications in type 1 diabetes may in part be explained by changes in haemostatic function. In the present study, we investigated the fibrin clot properties in patients with type 1 diabetes in relation to sex and microvascular complications. The study included 236 patients (107 women) aged between 20-70 years and without any history of cardiovascular disease. Fibrin clot properties, assessed by determination of the permeability coefficient (Ks) and turbidimetric clotting and lysis assays, did not differ between men and women. Compared with men, women had worse glycaemic control as well as higher levels of prothrombin fragment 1+2 and peak thrombin generation in vitro, indicating increased thrombin generation both in vivo and in vitro. Subgroup analyses of patients younger than 30 years revealed less permeable fibrin clots and prolonged lysis time in females compared with age-matched men. Patients with microvascular complications had higher fibrinogen concentrations and denser and less permeable fibrin clots. Thus, we conclude that in vitro fibrin clot properties in patients with type 1 diabetes without cardiovascular disease are not different between the sexes, but associate with prevalence of microvascular complications. Tighter fibrin clot formation in younger women, as suggested by our results, may affect their future cardiovascular risk and should be investigated in a larger population.

  14. Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization

    Directory of Open Access Journals (Sweden)

    Jarel K. Gandhi

    2015-09-01

    Full Text Available Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC, within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.

  15. A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

    Directory of Open Access Journals (Sweden)

    Mustafa Tunalı

    2014-01-01

    Full Text Available We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF. The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun’s leukocyte- and platelet-rich fibrin (L-PRF method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF was processed with a scanning electron microscope (SEM. The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

  16. A novel platelet concentrate: titanium-prepared platelet-rich fibrin.

    Science.gov (United States)

    Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Yaprak, Emre; Toker, Hülya; Fıratlı, Erhan

    2014-01-01

    We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

  17. Identification of quantitative trait loci for fibrin clot phenotypes: The EuroCLOT study

    Science.gov (United States)

    Williams, Frances MK; Carter, Angela M; Kato, Bernet; Falchi, Mario; Bathum, Lise; Surdulescu, Gabriela; Kyvik, Kirsten Ohm; Palotie, Aarno; Spector, Tim D; Grant, Peter J

    2012-01-01

    Objectives Fibrin makes up the structural basis of an occlusive arterial thrombus and variability in fibrin phenotype relates to cardiovascular risk. The aims of the current study from the EU consortium EuroCLOT were to 1) determine the heritability of fibrin phenotypes and 2) identify QTLs associated with fibrin phenotypes. Methods 447 dizygotic (DZ) and 460 monozygotic (MZ) pairs of healthy UK Caucasian female twins and 199 DZ twin pairs from Denmark were studied. D-dimer, an indicator of fibrin turnover, was measured by ELISA and measures of clot formation, morphology and lysis were determined by turbidimetric assays. Heritability estimates and genome-wide linkage analysis were performed. Results Estimates of heritability for d-dimer and turbidometric variables were in the range 17 - 46%, with highest levels for maximal absorbance which provides an estimate of clot density. Genome-wide linkage analysis revealed 6 significant regions with LOD>3 on 5 chromosomes (5, 6, 9, 16 and 17). Conclusions The results indicate a significant genetic contribution to variability in fibrin phenotypes and highlight regions in the human genome which warrant further investigation in relation to ischaemic cardiovascular disorders and their therapy. PMID:19150881

  18. Prevalence of sealants in relation to dental caries on the permanent molars of 12 and 15-year-old Greek adolescents. A national pathfinder survey

    Directory of Open Access Journals (Sweden)

    Mamai-Homata Eleni

    2011-02-01

    Full Text Available Abstract Background The use of sealants as an effective measure for the prevention of pit and fissure caries in children has been well documented by several studies; either they are used on an individual or on a public health basis. In order to plan and establish a national preventive program with sealants in a community, it is mandatory to know the epidemiological pattern of caries along with other variables influencing their use and effectiveness. Aims: To assess the utilization and distribution pattern of pit and fissure sealants on the first and second permanent molars of Greek adolescents and to evaluate whether the existing usage of sealants and some socio-demographic factors are correlated to caries prevalence on the population examined Methods A stratified cluster sample of 2481 Greek adolescents was selected according to WHO guidelines (1224 twelve and 1,257 fifteen-year-old, living in urban and rural areas in 11 districts within the country. Five calibrated examiners carried out clinical examinations, recording caries experience at the dentine threshold (BASCD criteria and presence or absence of sealants along with Socio-demographic indicators associated with oral health. Mann Whitney and Pearson's chi-square non parametric tests were utilized for assessing the data. The level of significance was p Results Sealants utilization varied considerably within the different districts, with 8,3% of the 12 and 8,0% of the 15-year-old adolescents having at least one sealed molar. Sealants reduced DMFS scores by 11% in the 12-year-olds and by 24% in the 15-year-olds, while 15-year-old adolescents from rural areas had a statistically significant (p = 0.002 less chance of having sealants (71% compared to children from urban areas. Girls had higher chance to receive sealants in both age groups (26% for the 12 and 19% for the 15-year-old as well as patients that visited the dentist for prevention compared to those visiting the dentist because they

  19. Ultrastructural characteristics of fibrin clots from canine and feline platelet concentrates activated with calcium gluconate or calcium gluconate plus batroxobin

    OpenAIRE

    Silva, Raúl F; Carmona, Jorge U; Rezende, Cleuza MF

    2013-01-01

    Background The aim of this study was to use transmission electron microscopy to describe the ultrastructural characteristics of clots obtained from canine and feline platelet concentrates (PC) that had been activated with calcium gluconate (CG) or CG plus batroxobin (CGB). Platelets from fibrin clots were classified according their morphological changes. The area of the intercellular space (μm2), the area of the fibrin fibers (μm2), and the width of the fibrin fibers (μm) were determined for ...

  20. Percutaneous BioOrganic Sealing of Duodenal Fistulas: Case Report and Review of Biological Sealants with Potential Use in Interventional Radiology

    Energy Technology Data Exchange (ETDEWEB)

    Wadhwa, Vibhor, E-mail: vwadhwa1@jhmi.edu [Johns Hopkins University School of Medicine, Division of Vascular & Interventional Radiology (United States); Leeper, William R., E-mail: rob.leeper@jhmi.edu [Johns Hopkins University School of Medicine, Department of Surgery (United States); Tamrazi, Anobel, E-mail: atamraz1@jhmi.edu [Johns Hopkins University School of Medicine, Division of Vascular & Interventional Radiology (United States)

    2015-08-15

    Biological sealants are being increasingly used in a variety of surgical specialties for their hemostatic and sealing capabilities. However, their use in interventional radiology has not been widely reported. The authors describe a case of duodenal perforation occurring after 15 years of gastric bypass surgery, in whom surgical diversion was unsuccessfully attempted and the leakage was successfully controlled using percutaneous administration of a combination of biological and organic sealants.

  1. Fissure sealants: in vitro evaluation of abrasion wear and superficial roughness

    Directory of Open Access Journals (Sweden)

    Vanessa Pardi

    2008-06-01

    Full Text Available The aim of this study was to compare the in vitro wear and superficial roughness of four materials (Delton Dyract Flow, Dentsply; Filtek Flow, Vitremer, 3M ESPE used as fissure sealant in 32 extracted human molars divided in four groups (n = 8 after abrasion with toothbrush/dentifrice. Impressions of each occlusal surface were made to analyze wear and circular specimens were prepared to analyze the roughness. Teeth and specimens were mounted in a toothbrushing machine. The replicas were observed using a SEM to determine the superficial wear. Wear: there were no statistically significant differences either between Delton and Filtek Flow or between Dyract Flow and Vitremer. Roughness: there were no statistical differences between Filtek Flow and Dyract Flow, Dyract Flow and Vitremer, Vitremer and Delton., Considering the clinical practice, if caries activity is present the use of Vitremer is suggested not only for its well known fluoride release, but it presented good roughness results.

  2. Use of FloSeal Sealant in the Surgical Management of Tubal Ectopic Pregnancy

    Directory of Open Access Journals (Sweden)

    Mara Clapp

    2013-01-01

    Full Text Available Background. Surgery is sometimes required for the management of tubal ectopic pregnancies. Historically, surgeons used electrosurgery to obtain hemostasis. Topical hemostatic sealants, such as FloSeal, may decrease the reliance on electrosurgery and reduce thermal injury to the tissue. Case. A 33-year-old G1 P0 received methotrexate for a right tubal pregnancy. The patient became symptomatic six days later and underwent a laparoscopic right salpingotomy. After multiple unsuccessful attempts to obtain hemostasis with electrocoagulation, FloSeal was used and hemostasis was obtained. Six weeks later, a hysterosalpingogram (HSG confirmed tubal patency. The patient subsequently had an intrauterine pregnancy. Conclusion. FloSeal helped to achieve hemostasis during a laparoscopic salpingotomy and preserve tubal patency. FloSeal is an effective alternative and adjunct to electrosurgery in the surgical management of tubal pregnancy.

  3. Novel Chemically-Bonded Phosphate Ceramic Borehole Sealants (Ceramicretes) for Arctic Environments

    Energy Technology Data Exchange (ETDEWEB)

    Shirish Patil; Godwin A. Chukwu; Gang Chen; Santanu Khataniar

    2008-12-31

    Novel chemically bonded phosphate ceramic borehole sealant, i.e. Ceramicrete, has many advantages over conventionally used permafrost cement at Alaska North Slope (ANS). However, in normal field practices when Ceramicrete is mixed with water in blenders, it has a chance of being contaminated with leftover Portland cement. In order to identify the effect of Portland cement contamination, recent tests have been conducted at BJ services in Tomball, TX as well as at the University of Alaska Fairbanks with Ceramicrete formulations proposed by the Argonne National Laboratory. The tests conducted at BJ Services with proposed Ceramicrete formulations and Portland cement contamination have shown significant drawbacks which has caused these formulations to be rejected. However, the newly developed Ceramicrete formulation at the University of Alaska Fairbanks has shown positive results with Portland cement contamination as well as without Portland cement contamination for its effective use in oil well cementing operations at ANS.

  4. Coal-tar pavement sealants might substantially increase children's PAH exposures

    Science.gov (United States)

    Williams, E. Spencer; Mahler, Barbara J.; Van Metre, Peter C.

    2012-01-01

    Dietary ingestion has been identified repeatedly as the primary route of human exposure to polycyclic aromatic hydrocarbons (PAHs), seven of which are classified as probable human carcinogens (B2 PAHs) by the U.S. EPA. Humans are exposed to PAHs through ingestion of cooked and uncooked foods, incidental ingestion of soil and dust, inhalation of ambient air, and absorption through skin. Although PAH sources are ubiquitous in the environment, one recently identified PAH source stands out: Coal-tar-based pavement sealant—a product applied to many parking lots, driveways, and even playgrounds primarily in the central, southern, and eastern U.S.—has PAH concentrations 100–1000 times greater than most other PAH sources. It was reported recently that PAH concentrations in house dust in residences adjacent to parking lots with coal-tar-based sealant were 25 times higher than in residences adjacent to unsealed asphalt parking lots.

  5. Effectiveness of Dry Cow Therapy Comprising Antibiotic Treatment, Internal Teat Sealant, and α-Tocopherol Against New Intramammary Infections in Cows

    Directory of Open Access Journals (Sweden)

    Cengiz Mehmet

    2015-04-01

    Full Text Available The aim of this study was to evaluate the preventive effectiveness of dry cow therapy based on antibiotic, internal teat sealant, and α-tocopherol administered separately or in various combinations at drying-off The study was performed on 322 uninfected quarters of 95 cows originating from three dairy herds. The new intramammary infection rates after calving were measured to evaluate the effectiveness. The quarters were divided into six groups differing in treatment, namely: control group (group C, n = 40 and five treatment groups. Treatment groups were arranged as follows: group A (antibiotic alone, n = 81, group AS (antibiotic + sealant, n = 40, group AST (antibiotic + sealant + α-tocopherol, n = 40, group T (α-tocopherol alone, n = 40, group S (sealant alone, n = 81. New infection rate amounted to 47.5% in group C. The treatment in group AST significantly prevented from the occurrence of new intramammary infections (12.5%, P 0.05, although the use of the sealant alone (group S decreased the risk of new infection (24.7%, P 0.05. Increased α-tocopherol level (P < 0.05 was detected after calving in the quarters from cows that received α-tocopherol injections. In conclusion, the combination of antibiotic, internal teat sealant, and α-tocopherol used in dry cow therapy showed a significantly better preventive effect against new intramammary infections, than the therapeutics administered separately.

  6. Comparison of the Effect of Topical Acidulated Phosphate Fluoride Application on Surface Roughness of Two Fissure Sealants and One Flowable Composite

    Directory of Open Access Journals (Sweden)

    Leila Pishevar

    Full Text Available Introduction: fluoride application and fissure sealant therapy have an important cariostatic effect in pediatric dentistry. Investigations are indicative for effects of topical fluoride specially APF (Acidulated Phosphate Fluoride gel on the restorative materials. The purpose of this study was to assess the effect of repeated application of APF (1. 23% gel on the surface roughness of two fissure sealants and one flowable composite. Materials & Methods: In this experimental study, 81 specimens of two fissure sealants and one flowable composite were prepared, using special polymer mold in three groups of 27 including: unfilled resin sealant (Fissurite F, Voco, filled resin sealant (Fissurit FX, Voco and one flowable composite (Arabesk Flow, Voco. Then, these three groups were divided into three sub groups of 9 as follows: Group 1, 4, 7 (control: No treatment. Group 2, 5, 8: Single application of APF gel. Group 3, 6, 9: Six times application of APF gel. The APF gel was applied on the surface of specimens each time for 4 minutes. Then, the specimens were stored in the distilled water. Finally, the surface roughness of the sealants was measured by Profilometer. The statistical analysis was performed by 2-Way ANOVA & One-Way ANOVA. Results: The results of the study showed a significant statistical different between the surface roughness according to the type of material (P0.05. Conclusion: All three materials were resistant to the destructive effects of APF gel and no significant surface roughness was detected on them.

  7. A comparative study to evaluate the effect of fluoride releasing sealant cured by visible light, argon laers, and light emitting diode curing units: An in vitro study

    OpenAIRE

    Das U; Prashanth S

    2009-01-01

    In Pediatric Dentistry, the use of pit and fissure sealant is one of the essential forms of prevention. Pit and fissure caries may be substantially decreased by obliteration of these developmental defects in occlusal, buccal and lingual surfaces with resin based dental sealants. Visible light-curing units, or LCUs, are an integral part of modern adhesive dentistry" They are used to cure resin based composite restoration materials, resin modified glass-ionomer and pit and fissure sealants, as ...

  8. A safe and efficient method to retrieve mesenchymal stem cells from three-dimensional fibrin gels.

    Science.gov (United States)

    Carrion, Bita; Janson, Isaac A; Kong, Yen P; Putnam, Andrew J

    2014-03-01

    Mesenchymal stem cells (MSCs) display multipotent characteristics that make them ideal for potential therapeutic applications. MSCs are typically cultured as monolayers on tissue culture plastic, but there is increasing evidence suggesting that they may lose their multipotency over time in vitro and eventually cease to retain any resemblance to in vivo resident MSCs. Three-dimensional (3D) culture systems that more closely recapitulate the physiological environment of MSCs and other cell types are increasingly explored for their capacity to support and maintain the cell phenotypes. In much of our own work, we have utilized fibrin, a natural protein-based material that serves as the provisional extracellular matrix during wound healing. Fibrin has proven to be useful in numerous tissue engineering applications and has been used clinically as a hemostatic material. Its rapid self-assembly driven by thrombin-mediated alteration of fibrinogen makes fibrin an attractive 3D substrate, in which cells can adhere, spread, proliferate, and undergo complex morphogenetic programs. However, there is a significant need for simple cost-effective methods to safely retrieve cells encapsulated within fibrin hydrogels to perform additional analyses or use the cells for therapy. Here, we present a safe and efficient protocol for the isolation of MSCs from 3D fibrin gels. The key ingredient of our successful extraction method is nattokinase, a serine protease of the subtilisin family that has a strong fibrinolytic activity. Our data show that MSCs recovered from 3D fibrin gels using nattokinase are not only viable but also retain their proliferative and multilineage potentials. Demonstrated for MSCs, this method can be readily adapted to retrieve any other cell type from 3D fibrin gel constructs for various applications, including expansion, bioassays, and in vivo implantation.

  9. Fabrication and physical and biological properties of fibrin gel derived from human plasma.

    Science.gov (United States)

    Zhao, Haiguang; Ma, Lie; Zhou, Jie; Mao, Zhengwei; Gao, Changyou; Shen, Jiacong

    2008-03-01

    The fast development of tissue engineering and regenerative medicine drives the old biomaterials, for example, fibrin glue, to find new applications in these areas. Aiming at developing a commercially available hydrogel for cell entrapment and delivery, in this study we optimized the fabrication and gelation conditions of fibrin gel. Fibrinogen was isolated from human plasma by a freeze-thaw circle. Gelation of the fibrinogen was accomplished by mixing with thrombin. Absorbance of the fibrinogen/thrombin mixture at 550 nm as a function of reaction time was monitored by UV-VIS spectroscopy. It was found that the clotting time is significantly influenced by the thrombin concentration and the temperature, while less influenced by the fibrinogen concentration. After freeze-drying, the fibrin gel was characterized by scanning electron microscopy (SEM), revealing fibrous microstructure. Thermal gravimetric analysis found that the degradation temperature of the crosslinked fibrin gel starts from 288 degrees C, which is about 30 degrees C higher than that of the fibrinogen. The hydrogel has an initial water-uptake ratio of approximately 50, decreased to 30-40 after incubation in water for 11 h depending on the thrombin concentration. The fibrin gels lost their weights in PBS very rapidly, while slowly in DMEM/fetal bovine serum and DMEM. In vitro cell culture found that human fibroblasts could normally proliferate in the fibrin gel with spreading morphology. In conclusion, the fibrin gel containing higher concentration of fibrinogen (20 mg ml(-1)) and thrombin (5 U ml(-1)) has suitable gelation time and handling properties, and thus is applicable as a delivery vehicle for cells such as fibroblasts.

  10. Inhibition of fibrin polymerization by synthetic peptides corresponding to Aalpha195-205 and gamma69-77 sites of fibrin molecule

    NARCIS (Netherlands)

    Pozniak, T.A.; Urvant, L.P.; Gritsenko, P.; Chernishov, V.I.; Pydiura, N.A.; Lugovskoi, E.V.; Komisarenko, S.V.

    2014-01-01

    Using the idea of "proline brackets" we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: gamma69NPDESSKPN77, Bbeta228QPDSSVKPY236, Bbeta455RPFFPQ460 and Aalpha195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic

  11. Platelet rich fibrin and xenograft in treatment of intrabony defect

    Directory of Open Access Journals (Sweden)

    Saurav Panda

    2014-01-01

    Full Text Available For complete periodontal regeneration, delivery of growth factors in the local environment holds a great deal in adjunct to bone grafts. Platelet rich fibrin (PRF is considered as second generation platelet concentrate, consisting of viable platelets, releasing various growth factors such as platelet-derived growth factor, vascular endothelial growth factor, transforming growth factor, insulin-like growth factor, epidermal growth factor and basic fibroblast growth factor. Hence, this case report aims to investigate the clinical and radiological (bone fill effectiveness of autologous PRF along with the use of xenogenic bone mineral in the treatment of intra bony defects. Intrabony defect was treated with autologous PRF along with the use of xenogenic bone mineral. A decrease in probing pocket depth, gain in clinical attachment level and significant bone fill was observed at end of 6 months. The result obtained with the use of PRF may be attributed to the sustained and simultaneous release of various growth factors over a period of 7 days. In this case report, the positive clinical impact of additional application of PRF with xenogenic graft material in the treatment of periodontal intrabony defect was seen.

  12. The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: kinetic and microscopic studies.

    Science.gov (United States)

    Longstaff, Colin; Thelwell, Craig; Williams, Stella C; Silva, Marta M C G; Szabó, László; Kolev, Krasimir

    2011-01-13

    Regulation of tissue-type plasminogen activator (tPA) depends on fibrin binding and fibrin structure. tPA structure/function relationships were investigated in fibrin formed by high or low thrombin concentrations to produce a fine mesh and small pores, or thick fibers and coarse structure, respectively. Kinetics studies were performed to investigate plasminogen activation and fibrinolysis in the 2 types of fibrin, using wild-type tPA (F-G-K1-K2-P, F and K2 binding), K1K1-tPA (F-G-K1-K1-P, F binding), and delF-tPA (G-K1-K2-P, K2 binding). There was a trend of enzyme potency of tPA > K1K1-tPA > delF-tPA, highlighting the importance of the finger domain in regulating activity, but the differences were less apparent in fine fibrin. Fine fibrin was a better surface for plasminogen activation but more resistant to lysis. Scanning electron and confocal microscopy using orange fluorescent fibrin with green fluorescent protein-labeled tPA variants showed that tPA was strongly associated with agglomerates in coarse but not in fine fibrin. In later lytic stages, delF-tPA-green fluorescent protein diffused more rapidly through fibrin in contrast to full-length tPA, highlighting the importance of finger domain-agglomerate interactions. Thus, the regulation of fibrinolysis depends on the starting nature of fibrin fibers and complex dynamic interaction between tPA and fibrin structures that vary over time.

  13. Relation between in-vitro wear and nanomechanical properties of commercial light-cured dental composites coated with surface sealants

    Directory of Open Access Journals (Sweden)

    Emanuel Santos Jr

    2013-01-01

    Full Text Available This work investigates the correlation between the in-vitro wear resistance and the nanomechanical properties of dental sealants commercially available. Mechanical properties, namely hardness (H and elastic modulus (E, were assessed by nanoindentation technique. The coated samples presented lower H and E values than the Z250 composite resin substrate. Such measurements were used to calculate H/E ratios. Wear tests were carried out in water by using a pin-on-plate apparatus. Scars formed on the samples were qualitatively examined by optical microscopy, while their wear depths were measured by contact profilometry. Based on the findings, an empirical correlation between the wear depths and H/E was obtained. A high H/E ratio was associated to surfaces with enhanced wear resistance. For the tribological conditions here employed, the H/E ratio could be, therefore, considered a useful parameter for ranking the in-vitro wear of dental sealants.

  14. A novel hemostatic sealant composed of gelatin,transglutaminase and thrombin effectively controls liver trauma-induced bleeding in dogs

    Institute of Scientific and Technical Information of China (English)

    Xia XIE; Jiang-ke TIAN; Fa-qin LV; Rong WU; Wen-bo TANG; Yu-kun LUO; Ya-qin HUANG

    2013-01-01

    Aim:A novel hemostatic sealant based on the in situ gel formation of gelatin catalyzed by thrombin and crosslinked by transglutaminase was designed.The aim of this study was to investigate the efficacy of the hemostatic sealant in control of bleeding caused by liver trauma in dogs.Methods:Hepatic trauma that mimicked the grade Ⅲ-Ⅳ rupture of liver was made in 20 dogs.The traumatic lesion was topically administered the hemostatic sealant (treatment group,n=10),or a thrombin solution (control group,n=10).The time to achieve hemostasis and the blood loss were measured.Contrast-enhanced ultrasound (CEUS) examination was performed in each animal on d 3,d 7,and d 10 d postoperatively to study the healing of the lesions.Results:The mean time to achieve hemostasis in the treatment group was significantly shorter than that in the control group (1.20±0.33 vs 6.70±0.64 min,P<0.05).The mean blood loss in the treatment group was significantly less than that in the control group (47.22±8.61 vs 79.29±11.97 mL,P<0.05).In CEUS examination,the traumatic lesions in the treatment group became significantly smaller on d 3,and disappeared on d 7,whereas the lesions in the control group still existed on d 10.Ascites were never found during 10 d follow-up in the treatment group but were observed on d 3 and d 7 in the control group.Conclusion:Compared with thrombin,the novel hemostatic sealant shows much better efficacy in hemostasis and may promote wound healing in dog liver trauma.

  15. Influence of Light-Curing Mode on the Erosion Preventive Effect of Three Different Resin-Based Surface Sealants

    Directory of Open Access Journals (Sweden)

    Florian J. Wegehaupt

    2012-01-01

    Full Text Available Objectives. To investigate if reducing the light-curing time (while maintaining similar energy density of resin-based surface sealants influences their erosion-preventive potential and mechanical stability after thermomechanical loading. Methods. Dentine samples were treated as follows: group 1—untreated, groups 2–4—Seal&Protect, groups 5–7—experimental sealer, and groups 8–10—Syntac Classic system. Groups 2, 5 and 8 were light-cured for 10 s (1000 mW/cm2, groups 3, 6 and 9 for 7 s (1400 mW/cm2, and groups 4, 7, and 10 for 3 s (3200 mW/cm2. After water storage (7 d, first measurement was performed to evaluate baseline permeability of the sealants. After a thermomechanical loading (5000 cycles, 50/5°C, 12000 brushing strokes a second evaluation of permeability was conducted (measurement 2. Permeability was tested by storing the samples in HCl (pH 2.3; 24 h and measuring the dentine calcium release by atomic absorption spectroscopy. Results. For the first and second measurements, no influence of light-exposure time on permeability was observed (ANOVA: P>0.05. No significant difference in the stability of the respective sealants was observed when light-cured for different durations. Conclusion. Shortening the light-curing time, while maintaining energy density constant, has no influence on permeability and stability of the investigated sealants.

  16. Pit and Fissure Sealants in Young Adults: An Evaluation of Placement Time and Retention Rate Using Two Isolation Techniques.

    Science.gov (United States)

    1983-01-01

    described as "the single most important anatomical feature leading to the development of occlusal caries" (13). 4 Recent state and national surveys have...was caused due to placement of the sealant. One patient returned after five days and stated he was still having temporomandibular joint dysfunction...longitudinal study of caries development in initially caries-free naval recruits, J Dent Res 61:1405-1407, 1982. 15. Miller, N. D.: Preventive treatment of teeth

  17. PAH concentrations in lake sediment decline following ban on coal-tar-based pavement sealants in Austin, Texas

    Science.gov (United States)

    Van Metre, Peter C.; Mahler, Barbara J.

    2013-01-01

    Recent studies have concluded that coal-tar-based pavement sealants are a major source of polycyclic aromatic hydrocarbons (PAHs) in urban settings in large parts of the United States. In 2006, Austin, TX, became the first jurisdiction in the U.S. to ban the use of coal-tar sealants. We evaluated the effect of Austin’s ban by analyzing PAHs in sediment cores and bottom-sediment samples collected in 1998, 2000, 2001, 2012, and 2014 from Lady Bird Lake, the principal receiving water body for Austin urban runoff. The sum concentration of the 16 EPA Priority Pollutant PAHs (∑PAH16) in dated core intervals and surficial bottom-sediment samples collected from sites in the lower lake declined about 44% from 1998–2005 to 2006–2014 (means of 7980 and 4500 μg kg–1, respectively), and by 2012–2014, the decline was about 58% (mean of 3320 μg kg–1). Concentrations of ∑PAH16 in bottom sediment from two of three mid-lake sites decreased by about 71 and 35% from 2001 to 2014. Concentrations at a third site increased by about 14% from 2001 to 2014. The decreases since 2006 reverse a 40-year (1959–1998) upward trend. Despite declines in PAH concentrations, PAH profiles and source-receptor modeling results indicate that coal-tar sealants remain the largest PAH source to the lake, implying that PAH concentrations likely will continue to decline as stocks of previously applied sealant gradually become depleted.

  18. Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering

    Science.gov (United States)

    Liu, Jun; Xu, Hockin H.K.; Zhou, Hongzhi; Weir, Michael D.; Chen, Qianming; Trotman, Carroll Ann

    2012-01-01

    There has been little research on the seeding of human umbilical cord mesenchymal stem cells (hUCMSCs) in three-dimensional scaffolds for muscle tissue engineering. The objectives of this study were: (i) to seed hUCMSCs in a fibrin hydrogel containing fast-degradable microbeads (dMBs) to create macropores to enhance cell viability; and (ii) to investigate the encapsulated cell proliferation and myogenic differentiation for muscle tissue engineering. Mass fractions of 0–80% of dMBs were tested, and 35% of dMBs in fibrin was shown to avoid fibrin shrinkage while creating macropores and promoting cell viability. This construct was referred to as “dMB35”. Fibrin without dMBs was termed “dMB0”. Microbead degradation created macropores in fibrin and improved cell viability. The percentage of live cells in dMB35 reached 91% at 16 days, higher than the 81% in dMB0 (p muscle tissue engineering applications. PMID:22902812

  19. Excess Fibrin Deposits Decrease Fetal Weight of Pregnant Mice Infected by Plasmodium berghei

    Directory of Open Access Journals (Sweden)

    Desy Andari

    2014-05-01

    Full Text Available Low birth weight is commonly attributed to malaria in pregnancy, but the cellular and molecular mechanisms that underlie this poor birth outcome are incompletely understood. A universally described histopathological feature of placental malaria is excessive deposition of fibrin, the end-product of the coagulation cascade. This study was conducted to compare fibrin deposit in pregnant mice that infected by Plasmodium berghei (treatment group to the normal pregnant mice (control group and its association with fetal weight. This research is in vivo experimental laboratory study that used 18 pregnant Balb/c mice which divided to the control the group (8 mice and treatment group (9 mice infected by P.berghei. Placentas were staining with Haematoxylin-Eosin (HE for fibrin deposits examination whereas fetal weight was performed with Mettler analytical balance AE 50. Fetal weight of the treatment group was lower than those of the control group (t test, p=0,002. Fibrin deposits were increased in the treatment group (t test, p=0,005 and influenced weight of fetuses (Spearman r= -0,586, p= 0,014. Weights of fetuses are interfered by fibrin deposits during malaria infection.

  20. The application of plastic compression to modulate fibrin hydrogel mechanical properties.

    Science.gov (United States)

    Haugh, Matthew G; Thorpe, Stephen D; Vinardell, Tatiana; Buckley, Conor T; Kelly, Daniel J

    2012-12-01

    The inherent biocompatibility of fibrin hydrogels makes them an attractive material for use in a wide range of tissue engineering applications. Despite this, their relatively low stiffness and high compliance limits their potential for certain orthopaedic applications. Enhanced mechanical properties are desirable so as to withstand surgical handling and in vivo loading after implantation and additionally, can provide important cues to cells seeded within the hydrogel. Standard methods used to enhance the mechanical properties of biological scaffolds such as chemical or thermal crosslinking cannot be used with fibrin hydrogels as cell seeding and gel formation occurs simultaneously. The objective of this study was to investigate the use of plastic compression as a means to improve the mechanical properties of chondrocyte-seeded fibrin hydrogels and to determine the influence of such compression on cell viability within these constructs. It was found that the application of 80% strain to fibrin hydrogels for 30 min (which resulted in a permanent strain of 47.4%) produced a 2.1-fold increase in the subsequent compressive modulus. Additionally, chondrocyte viability was maintained in the plastically compressed gels with significant cellular proliferation and extracellular matrix accumulation observed over 28 days of culture. In conclusion, plastic compression can be used to modulate the density and mechanical properties of cell-seeded fibrin hydrogels and represents a useful tool for both in theatre and in vitro tissue engineering applications.

  1. Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

    Directory of Open Access Journals (Sweden)

    Langer, Stefan

    2015-12-01

    Full Text Available Aim: Fibrin glue becomes a more and more routinely used tool for stabilization of microanastomoses and nerve repair. This paper summarizes the technical properties and advantages of its use in a wide variety of microsurgical contexts, and includes an exemplary limb reconstructive case.Patients and methods: A total of 131 patients who had undergone elective and emergency microsurgery mainly of the limbs were retrospectively analyzed, as was the use of free flaps.Results: The use of fibrin glue allows for proper positioning of anastomoses and repaired nerves. No torsion of the pedicle could be seen. The flap survival rated >94%. The fibrin glue could stay in place in >99%. In the rare case of revision, the fibrin glue could easily be removed without damaging the region of the microanastomosis.Conclusion: Fibrin glue should not be used to repair insufficient, i.e., leaking anastomoses, but it does protect the site of anastomosis from tissue and fluid pressure. It prevents the pedickle from torsion and its use facilitates relocation of the microanastomoses in cases of revision surgery.

  2. Modulation of 3D Fibrin Matrix Stiffness by Intrinsic Fibrinogen–Thrombin Compositions and by Extrinsic Cellular Activity

    Science.gov (United States)

    Duong, Haison; Wu, Benjamin

    2009-01-01

    Fibrin is a substance formed through catalytic conversion of coagulation constituents: fibrinogen and thrombin. The kinetics of the two constituents determines the structural properties of the fibrin architecture. We have shown previously that changing the fibrinogen and thrombin concentrations in the final three-dimensional (3D) fibrin matrix influenced cell proliferation and differentiation. In this study, we further examined the effect of changing fibrinogen and thrombin concentrations in the absence or presence of fibroblasts on the structural modulus or stiffness of 3D fibrin matrices. We have prepared fibroblast-free and fibroblast-embedded 3D fibrin matrices of different fibrinogen and thrombin formulations, and tested the stiffness of these constructs using standard mechanical testing assays. Results showed that there was a corresponding increase in stiffness with increasing thrombin and fibrinogen concentrations; the increase was more notable with fibrinogen and to a lesser degree with thrombin. The effect of fibroblasts on the stiffness of the fibrin construct was also examined. We have observed a small increase in the stiffness of the fibroblast-incorporated fibrin construct as they proliferated and exhibited spreading morphology. To our knowledge, this is the first comprehensive report detailing the relationship between fibrinogen and thrombin concentrations, cell proliferation, and stiffness in 3D fibrin matrices. The data obtained may lead to optimally design suitable bioscaffolds where we can control both cell proliferation and structural integrity for a variety of tissue engineering applications. PMID:19309239

  3. [Results of fibrin clot application for acceleration of regeneration of the damaged mandible in experiment].

    Science.gov (United States)

    Maĭborodin, I V; Kolesnikov, I S; Shevela, A I; Sheplev, B V; Drovosekov, M N; Toder, M S

    2011-01-01

    The processes of regeneration of the damaged rat bottom jaw bone after application of enriched thrombocytes a fibrin clot were studied by morphological and radiovisiographic methods. At a natural course of regeneration the artificial aperture of bone was filled with blood and there the blood clot was formed. After 1 week the separate bone islets of a young tissue occurred in bone defect. In 2-3 weeks the aperture in a bottom jaw bone was completely closed by a young bone tissue. After operation with filling of bone bottom jaw defect by fibrin clot there was no formation of a blood clot. Already after 1 week the bone tissue defect was filled by the merged islets of again generated bone. By second week after fibrin use the further formation of bone tissue in defect and formation of a bone callosity was noted.

  4. Crosslinked fibrin gels for tissue engineering: two approaches to improve their properties.

    Science.gov (United States)

    Gamboa-Martínez, Tatiana C; Luque-Guillén, Victoria; González-García, Cristina; Gómez Ribelles, José Luis; Gallego-Ferrer, Gloria

    2015-02-01

    Fibrin is a protein that can be used as an ideal scaffolding material to promote tissue regeneration. In order to enhance its physical properties in this study a natural crosslinker, genipin (GP), was employed with the aim to obtain a hydrogel with tuneable properties for tissue engineering applications. The fibrin gel was crosslinked by two different methods using four concentrations of GP to get a stable hydrogel network. Crosslinking density, mechanical properties, swelling, and enzymatic degradation of the hydrogels were tested for each GP content and method employed. The method I: Crosslinking after gel formation promotes a high crosslinking and retains the gel shape for long term whilst the method II: Simultaneous gel formation and crosslinking improves the mechanical properties of the gel. This study confirms the use of GP at different concentrations as a suitable crosslinker of fibrin that promotes the cellular viability of L929 for 21 days of in vitro culture.

  5. Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft

    Directory of Open Access Journals (Sweden)

    Vinita Jain

    2012-01-01

    Full Text Available Platelet-rich fibrin has long been used as a wound healing therapy in skin wounds and recently evidence has suggested its usage in oral cavity for different treatment procedures. This article proposes an overview of use of platelet-rich fibrin in management of complicated oral wounds. Excessive hemorrhage of the donor area, necrosis of epithelium, and morbidity associated with donor site have been described as the possible complications after harvesting subepithelial connective tissue graft, but little has been mentioned about their management. The article includes a case report of a 45-year-old male patient who showed a delayed wound healing after subepithelial connective tissue graft harvestation, which was treated with platelet-rich fibrin.

  6. Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time.

    Science.gov (United States)

    Perez, Amanda G M; Rodrigues, Ana A; Luzo, Angela C M; Lana, José F S D; Belangero, William D; Santana, Maria H A

    2014-08-01

    Fibrin networks are obtained through activation of platelet-rich plasma (PRP) for use in tissue regeneration. The importance of fibrin networks relies on mediation of release of growth factors, proliferation of tissue cells and rheological properties of the fibrin gels. Activation of PRP usually involves the decomposition of fibrinogen by agonists, in a wide range of concentrations. Therefore fibrin networks with a large structural diversity are formed, making comparative evaluations difficult. In order to standardize the fibrin networks, we used the statistical techniques central composite rotatable design and response-surface analysis, to correlate the radius of the fibers with the ratios between the agonists (autologous serum/calcium chloride) and agonist/PRP. From an individual and interactive analysis of the variables, architectures characterized by thick, medium and thin fibers were delineated on the response-surface. Furthermore, the architectures were correlated with coagulation time. This approach is valuable for standardizing the PRP preparation for clinical applications.

  7. Determination of fibrin glue with antibiotics on collagen production in colon anastomosis

    Directory of Open Access Journals (Sweden)

    Stanojković Zoran

    2008-01-01

    Full Text Available Background/Aim. Fibrin glue is used as a matrix for local application of antibiotics. The aim of this study was to determine whether application of fibrin glue in combination with antibiotics can strengthen collagen production, prevent dehiscence of colon anastomoses due to infection, and reduce frequency of mortality and morbidity comparing to the control group and the group with fibrin glue application. Methods. The adult male Wistar rats divided into three groups were used in the experiment. The group 1 was the control one (after partial colon resection, colonic anastomoses performed were not treated, while to the group 2 and the group 3 were applied fibrin glue and fibrin glue with antibiotics (clindamycin and ceftriaxon on the site of anastomoses, respectively. Quality of colonic anastomoses were estimated by means of determination of collagen (L-hydroxyproline amount in the collon wall with anastomoses and histological analysis of this colon segment using light and electronic microscope on the days 5, 7 and 13 postoperatively. Results. The highest morbidity rate was registered in the group 1 (30%, then in the group 2 (13.3% and the lowest one in the group 3 (3.33%; p < 0,05 vs group 1. Mortality rate was significantly higher in the group 1 than in the group 3 (20% and 0%, respectively; p < 0,05. In the postoperative course, the highest concentrations of collagen in the colon wall on the site of anastomoses, which was confirmed by both light and electronic microscopy, were found in the group 3. Conclusion. The application of fibrin glue with antibiotics on colon anastomoses reduces the number of dehiscence, provides good mechanical protection and shorten the time of anastomoses healing.

  8. Recombinant human bone morphogenetic protein-2 suspended in fibrin glue enhances bone formation during distraction osteogenesis in rabbits

    Science.gov (United States)

    Li, Yunfeng; Li, Rui; Hu, Jing; Song, Donghui; Jiang, Xiaowen

    2016-01-01

    Introduction Bone morphogenetic protein-2 (BMP-2) has high potential for bone formation, but its in vivo effects are unpredictable due to the short life time. This study was designed to evaluate the effects of recombinant human (rh) BMP-2 suspended in fibrin on bone formation during distraction osteogenesis (DO) in rabbits. Material and methods The in vitro release kinetics of rhBMP-2 suspended in fibrin was tested using an enzyme-linked immunosorbent assay. Unilateral tibial lengthening for 10 mm was achieved in 48 rabbits. At the completion of osteodistraction, vehicle, fibrin, rhBMP-2 or rhBMP-2 suspended in fibrin (rhBMP-2 + fibrin) was injected into the center of the lengthened gap, with 12 animals in each group. Eight weeks later, the distracted callus was examined by histology, micro-CT and biomechanical testing. Radiographs of the distracted tibiae were taken at both 4 and 8 weeks after drug treatment. Results It was found that fibrin prolonged the life span of rhBMP-2 in vitro with sustained release during 17 days. The rhBMP-2 + fibrin treated animals showed the best results in bone mineral density, bone volume fraction, cortical bone thickness by micro-CT evaluation and mechanical properties by the three-point bending test when compared to the other groups (p < 0.05). In histological images, rhBMP-2 + fibrin treatment showed increased callus formation and better gap bridging compared to the other groups. Conclusions The results of this study suggest that fibrin holds promise to be a good carrier of rhBMP-2, and rhBMP-2 suspended in fibrin showed a stronger promoting effect on bone formation during DO in rabbits. PMID:27279839

  9. El fibrinógeno como factor de riesgo de enfermedad aterotrombótica

    OpenAIRE

    Julio César Fernández Travieso

    2009-01-01

    La disfunción endotelial y la inflamación representan un decisivo papel desde las etapas tempranas de la aterosclerosis hasta la aparición de los síndromes coronarios agudos, en los cuales el fibrinógeno ha demostrado tener una activa participación. Además, en concentraciones elevadas se relaciona con la trombosis y ha sido demostrado que es un factor independiente de riesgo cardiovascular. Hoy día, concentraciones elevadas de fibrinógeno junto con la proteína C-reactiva, la lipoproteína (a) ...

  10. Nitrogenous subcutaneous emphysema caused by spray application of fibrin glue during retroperitoneal laparoscopic surgery.

    Science.gov (United States)

    Matsuse, Shinji; Maruyama, Atsushi; Hara, Yoshiki

    2011-06-01

    We report a case of a patient treated by retroperitoneoscopic partial nephrectomy who developed nitrogenous subcutaneous emphysema (SCE) as a complication. The use of a nitrogen gas-pressured fibrin tissue adhesive applied as a spray caused excessively increased pressure in the closed retroperitoneal space and resulted in widespread SCE with protracted clinical course. To the best of our knowledge, this is the first report of nitrogenous SCE associated with pneumoperitoneum. The clinical significance of nitrogenous SCE is emphasized, and the risks associated with the use of fibrin glue as a spray during laparoscopic surgery are discussed.

  11. Use of platelet-rich fibrin over skin wounds: Modified secondary intention healing

    Directory of Open Access Journals (Sweden)

    Chirag B Desai

    2013-01-01

    Full Text Available The healing process after any surgical intervention has always posed a challenge for the surgeons. In spite of the advances in wound closure techniques and devices, there is a crucial need for newer methods of enhancing the healing process to achieve optimal outcomes. Fibrin adhesives and platelet concentrates have proven useful in various treatment modalities in the fields of microvascular and plastic surgery. This case report shows its unique use in the field of maxillofacial and cutaneous surgery. It shows an innovative technique of enhancement of skin wound healing by local application of platelet-rich fibrin.

  12. Evaluation of asbestos exposure within the automotive repair industry: a study involving removal of asbestos-containing body sealants and drive clutch replacement.

    Science.gov (United States)

    Blake, Charles L; Dotson, G Scott; Harbison, Raymond D

    2008-12-01

    Two independent assessments were performed of airborne asbestos concentrations generated during automotive repair work on vintage vehicles . The first involved removal of asbestos-containing seam sealant, and the second involved servicing of a drive clutch. Despite the relatively high concentrations (5.6-28%) of chrysotile fibers detected within bulk samples of seam sealant, the average asbestos concentration for personal breathing zone (PBZ) samples during seam sealant removal was 0.006 f/cc (fibers/cubic centimeter of air). Many other air samples contained asbestos at or below the analytical limit of detection (LOD). Pneumatic chiseling of the sealant material during removal resulted in 69% of area air samples containing asbestos. Use of this impact tool liberated more asbestos than hand scraping. Asbestos fibers were only detected in air samples collected during the installation of a replacement clutch. The highest asbestos corrected airborne fiber concentration observed during clutch installation was 0.0028 f/cc. This value is approximately 100 times lower than Occupational Safety and Health Administration's (OSHA) permissible exposure limit (PEL) of 0.1f/cc. The airborne asbestos concentrations observed during the servicing of vintage vehicles with asbestos-containing seam sealant and clutches are comparable to levels reported for repair work involving brake components and gaskets.

  13. Investigation of Performance of SCN-1 Pure Glass as Sealant Used in SOFC

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Wenning N.; Sun, Xin; Stephens, Elizabeth V.; Khaleel, Mohammad A.

    2010-03-01

    As its name implies, self-healing glass seal has the potential of restoring its mechanical properties upon being reheated to stack operating temperature, even when it has experienced some cooling induced damage/crack at room temperature. Such a self-healing feature is desirable for achieving high seal reliability during thermal cycling. On the other hand, self-healing glass is also characterized by its low mechanical stiffness and high creep rate at the typical operating temperature of SOFCs. Therefore, from a design’s perspective, it is important to know the long term geometric stability and thermal mechanical behaviors of the self-healing glass under the stack operating conditions. These predictive capabilities will guide the design and optimization of a reliable sealing system that potentially utilizes self-healing glass as well as other ceramic seal components in achieving the ultimate goal of SOFC. In this report, we focused on predicting the effects of various generic seal design parameters on the stresses in the seal. For this purpose, we take the test cell used in the leakage test for compliant glass seals conducted in PNNL as our initial modeling geometry. The effect of the ceramic stopper on the geometry stability of the self-healing glass sealants is studied first. Then we explored the effect of various interfaces such as stopper and glass, stopper and PEN, as well stopper and IC plate, on the geometry stability and reliability of glass during the operating and cooling processes.

  14. Fire-retardant and fire-barrier poly(vinyl acetate composites for sealant application

    Directory of Open Access Journals (Sweden)

    2010-02-01

    Full Text Available Fire-retardant ceramifying poly(vinyl acetate (PVAc sealants have been prepared. The degradation of PVA was integrated with the action of the fire retardants to reduce flammable gases, produce carbonaceous char and convert the fillers into a self-supporting ceramic barrier. PVA is readily degraded by elimination of acetic acid, yielding a char that provides a transitory phase as the filler particles fuse into a ceramic mass. Acetic acid is eliminated at similar temperature to the release of water from magnesium hydroxide fire-retardant, thereby diluting flammable acetic acid. The residual oxide from the fire-retardant filler and structural filler are fused by a flux, zinc borate. The degradative and ceramifying processes were characterised using thermogravimetry, infrared spectroscopy, scanning electron microscopy and ceramic strength. Thermogravimetry of the composites was compared with additive mass loss curves calculated from the components. Deviations between the experimental and additive curves revealed interactions between the components in the composites. The modulus of the PVAc composites and the strength of their ceramic residues after combustion were determined.

  15. Marginal microleakage between fluoride-containing resin sealant and glass ionomer sealant in vitro%含氟树脂封闭剂与玻璃离子封闭剂边缘微渗漏的体外研究

    Institute of Scientific and Technical Information of China (English)

    刘桥; 林居红; 陈丹; 张红梅; 马文竹; 许小辉

    2013-01-01

    Objective:To observe and compare the state of marginal mieroleakage between fluoride-containing resin sealant and glass ionomer sealant in vitro. Methods:Totally 30 newly extracted noncarious human the third molars were randomly divided into three groups with 10 samples in each group. The occlusional surfaces of the specimens were sealed with ClinproTMSealant,Fuji IX GP and Concise, respectively. After 125 cycles of thermo-cycling, all specimens were immersed in 1% solution of methylene blue dye for 48 h then the microleakage of each specimen was measured under stereomicroscope. Results; Results of dye penetration revealed that there was no significant difference in microleakage among two test groups and control group(P>0.05) and there was no significant difference between two test groups(P>0.05),either. Conclusions : All three types of pit and fissure sealants showed lower microleakage. The tooth surface fitness of glass ionomer cement(Fuji IX GP) is comparatively similar to resin sealants,with satisfactory sealing ability. It's worth promoting glass ionomer material as pit and fissure sealant in clinics.%目的:观察和比较含氟树脂封闭剂与玻璃离子封闭剂的边缘微渗漏情况.方法:将30个无龋新鲜拔出的人类第3磨牙随机分为3组,每组10个标本,分别用含氟树脂封闭剂(ClinproTM Sealant),玻璃离子封闭剂(Fuji Ⅸ GP)和传统树脂封闭剂(Concise)对牙齿(牙合)面进行窝沟封闭,经125次热循环处理后,将所有标本浸泡于1%亚甲基蓝溶液中48 h,采用体视显微镜观察封闭剂边缘微渗漏情况.结果:染料渗透结果显示两实验组(ClinproTM Sealant组、FujiⅨGP组)与对照组(Concise组)材料边缘微渗漏比较,差异均无统计学意义(P>0.05);两实验组比较,差异也无统计学意义(P>0.05).结论:3种窝沟封闭材料均显示出较低的边缘微渗漏,并且玻璃离子水门汀FujiⅨGP具有与树脂类封闭剂相似的牙面密合度,封闭性能良

  16. Perivenous application of fibrin glue prevents the early injury of jugular vein graft to arterial circulation in rabbits

    Institute of Scientific and Technical Information of China (English)

    WAN Li; LI Dian-yuan; YANG Bing; WU Qing-yu

    2006-01-01

    Background Placement of an external support has been reported to prevent intimal hyperplasia of vein grafts. However, it is limited by potential complications. In the present study, we investigated the effect of fibrin glue on preventing vein graft failure as perivenous application. Methods Twenty-four rabbits were divided into non-supported group (n=12) and fibrin glue group (n=12). All animals underwent unilateral jugular vein into common carotid artery interposition grafting and then fibrin glue was applied as perivenous support. Samples of tissues were harvested after 4 weeks. Results The vein grafts with fibrin glue demonstrated a statistically significant decrease in proliferating cell nuclear antigen in the medial/intimal region [13.38% (11.26%-15.11%)] compared with non-supported vein grafts [31.22% (27.15%-35.98%)] (P<0.001). Light microscopy showed remarkable attenuation of endothelial cell loss and numerous microvessels in neoadventitia in the fibrin glue group compared with the non-supported group. The smooth muscle cells migrated into adventitia significantly in fibrin glue group, whereas the smooth muscle cells migrated into intima in non-supported group.Conclusion Perivenous support of vein graft with fibrin glue in vivo can attenuate the severe injury encountered in the non-supported vein grafts exposed to artery.

  17. Optimization of acidic fibroblast growth factor (FGF-1) and its delivery through a modified degradable fibrin scaffold

    Science.gov (United States)

    Pandit, Abhay Smashikant

    The aim of this investigation was to develop a degradable fibrin wound dressing that can deliver an optimized dose of acidic fibroblast growth factor (FGF-1). This aim led to three distinct phases of study. In the first phase, a structurally modified fibrin degradable scaffold was developed and tested in a rabbit ear ulcer model. A significant increase in the angiogenic and fibroblastic response with a corresponding decrease in healing time was seen in the modified fibrin-treated ulcers as compared with untreated ulcers and ulcers treated with non-modified fibrin systems. In the second phase of the study, a biochemical factor, FGF-1, was added to this scaffold. An optimal dose of 8 mug of FGF-1 was determined to be required to initiate a desired wound-healing response in a rabbit ear ulcer model, based on an enhanced angiogenic and fibroblastic response and an increased epithelialization rate. The objective of the last phase was to investigate the efficacy of a modified scaffold as a vehicle for FGF-1. In vivo testing was conducted in a full-thickness defect model in a rabbit. Improvements were seen in the angiogenic and fibroblastic responses in the FGF-1/modified fibrin treatment group and, hence, FGF-1/modified fibrin was the preferred treatment. In conclusion, the modified fibrin/FGF-1 matrix served as a suitable vehicle for the growth factor, providing a desired healing response and a desirable release rate and, thus, was determined to be an effective scaffold.

  18. 硅烷改性聚醚密封胶剪切强度的研%Study on shear strength of silane modified polyether sealant

    Institute of Scientific and Technical Information of China (English)

    王翠花; 赵瑞; 韩胜利

    2014-01-01

    以MS聚合物为粘料,添加填料、脱水剂、偶联剂、催化剂等助剂,制备一种单组分硅烷改性聚醚密封胶。研究了MS聚合物、填料、固化条件、胶层厚度对剪切强度的影响。%The one component silane modified polyether sealant was prepared with MS polymer as the binder,fillers,water absorbing agent,coupling agent and curing catalyst.This paper mainly studied the effects of the MS polymer,fillers,curing conditions and thickness of sealant layer on the shear strength of the sealant.

  19. Simultaneous sinus lift and implantation using platelet-rich fibrin as sole grafting material.

    Science.gov (United States)

    Jeong, Seung-Mi; Lee, Chun-Ui; Son, Jeong-Seog; Oh, Ji-Hyeon; Fang, Yiqin; Choi, Byung-Ho

    2014-09-01

    Recently, several authors have shown that simultaneous sinus lift and implantation using autologous platelet-rich fibrin as the sole filling material is a reliable procedure promoting bone augmentation in the maxillary sinus. The aim of this study was to examine the effect of simultaneous sinus lift and implantation using platelet-rich fibrin as the sole grafting material on bone formation in a canine sinus model. An implant was placed after sinus membrane elevation in the maxillary sinus of six adult female mongrel dogs. The resulting space between the membrane and sinus floor was filled with autologous platelet-rich fibrin retrieved from each dog. The implants were left in place for six months. Bone tissue was seen at the lower part of the implants introduced into the sinus cavity. The height of the newly formed bone around the implants ranged from 0 mm to 4.9 mm (mean; 2.6 ± 2.0 mm) on the buccal side and from 0 mm to 4.2 mm (mean; 1.3 ± 1.8 mm) on the palatal side. The findings from this study suggest that simultaneous sinus lift and implantation using platelet-rich fibrin as sole grafting material is not a predictable and reproducible procedure, especially with respect to the bone formation around the implants in the sinus cavity.

  20. Histological assessment of titanium and polypropylene fiber mesh implantation with and without fibrin tissue glue

    NARCIS (Netherlands)

    Hallers, E.J.O. ten; Jansen, J.A.; Marres, H.A.M.; Rakhorst, G.; Verkerke, G.J.

    2007-01-01

    Polypropylene (PP) and titanium (Ti) meshes are well-known surgical implants that provoke a relative low foreign body reaction. Firm stabilization of the implant is important to prevent migration and subsequent failure of the operation. Fibrin tissue glues are commercially available adhesives and ar

  1. Fibrin glue used successfully in peritoneal dialysis catheter leakage in children.

    NARCIS (Netherlands)

    Rusthoven, E.; Kar, N.C.A.J. van de; Monnens, L.A.H.; Schröder, C.H.

    2004-01-01

    BACKGROUND: Acute renal failure in infants and small children is generally treated with peritoneal dialysis (PD). Dialysis has to be started immediately after catheter implantation. Early dialysate leakage can complicate the effectiveness of dialysis. Fibrin glue applied to the external part of the

  2. Application of fibrin glue to damaged bladder mucosa in a case of BK viral hemorrhagic cystitis.

    Science.gov (United States)

    Purves, J Todd; Graham, Michael L; Ramakumar, Sanjay

    2005-09-01

    BK virus is a common cause of severe hemorrhagic cystitis refractory to standard treatment. We describe a technique to achieve hemostasis after failed conservative therapy using fibrin glue applied suprapubically while visualizing and insufflating the bladder through a cystoscope. Long-term hemostasis was achieved using this novel procedure.

  3. Combined treatment of iatrogenic rectourethral fistula with endoscopic fibrin glue application and clipping.

    Science.gov (United States)

    Dolay, Kemal; Aras, Bekir; Tuğcu, Volkan; Ozbay, Bedi; Aygün, Erşan; Taşçi, Ali Ihsan

    2007-04-01

    Rectourethral fistula (RUF) formation is a rare complication of prostatic surgery and other pelvic surgical procedures. The results of operations to correct RUF are not always satisfying, with a particular risk of recurrent breakdown or stricture formation at the site of the urethral closure. We present a case of a small RUF treated videoendoscopically with fibrin-glue application combined with endoscopic clipping.

  4. The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots

    Directory of Open Access Journals (Sweden)

    Martha M. Murray

    2011-08-01

    Full Text Available Recently there has been a great deal of interest in the use of biomaterials to stimulate wound healing. This is largely due to their ability to centralize high concentrations of compounds known to promote wound healing at a needed location. Joints present a unique challenge to using scaffolds because of the presence of enzymes in synovial fluid which are known to degrade materials that would be stable in other parts of the body. The hypothesis of this study was that atelocollagen scaffolds would have greater resistance to enzymatic degradation than scaffolds made of gelatin, fibrin and whole blood. To test this hypothesis, collagen and fibrin-based scaffolds were placed in matrix metallopeptidase-1 (MMP-1, elastase, and plasmin solutions at physiologic concentrations, and the degradation of each scaffold was measured at varying time points. The atelocollagen scaffolds had a significantly greater resistance to degradation by MMP-1, elastase and plasmin over the fibrin based scaffolds. The results suggest that atelocollagen-based scaffolds may provide some protection against premature degradation by synovial fluid enzymes over fibrin-based matrices.

  5. Lyophilized Platelet-Rich Fibrin (PRF Promotes Craniofacial Bone Regeneration through Runx2

    Directory of Open Access Journals (Sweden)

    Qi Li

    2014-05-01

    Full Text Available Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF. LPRF caused a 4.8-fold ± 0.4-fold elevation in Runt-related transcription factor 2 (Runx2 expression in alveolar bone cells, compared to a 3.6-fold ± 0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p < 0.001 when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

  6. Modelling of platelet-fibrin clot formation in flow with a DPD-PDE method.

    Science.gov (United States)

    Tosenberger, A; Ataullakhanov, F; Bessonov, N; Panteleev, M; Tokarev, A; Volpert, V

    2016-02-01

    The paper is devoted to mathematical modelling of clot growth in blood flow. Great complexity of the hemostatic system dictates the need of usage of the mathematical models to understand its functioning in the normal and especially in pathological situations. In this work we investigate the interaction of blood flow, platelet aggregation and plasma coagulation. We develop a hybrid DPD-PDE model where dissipative particle dynamics (DPD) is used to model plasma flow and platelets, while the regulatory network of plasma coagulation is described by a system of partial differential equations. Modelling results confirm the potency of the scenario of clot growth where at the first stage of clot formation platelets form an aggregate due to weak inter-platelet connections and then due to their activation. This enables the formation of the fibrin net in the centre of the platelet aggregate where the flow velocity is significantly reduced. The fibrin net reinforces the clot and allows its further growth. When the clot becomes sufficiently large, it stops growing due to the narrowed vessel and the increase of flow shear rate at the surface of the clot. Its outer part is detached by the flow revealing the inner part covered by fibrin. This fibrin cap does not allow new platelets to attach at the high shear rate, and the clot stops growing. Dependence of the final clot size on wall shear rate and on other parameters is studied.

  7. Supplementation of fibrin gels with sodium chloride enhances physical properties and ensuing osteogenic response.

    Science.gov (United States)

    Davis, H E; Miller, S L; Case, E M; Leach, J K

    2011-02-01

    Modifying the relative concentrations of fibrinogen and thrombin can control the physical properties of fibrin gels, while the viability of associated cells has been linked to the gel's final network structure. It was hypothesized that increasing the gel ionic strength during fabrication through supplementation with sodium chloride (NaCl) would provide an improved approach for tailoring the physical properties of fibrin gels and maintaining the viability and osteogenic potential of entrapped cells. Fibrin gels were formed by mixing fibrinogen, thrombin and calcium chloride with varying masses of NaCl (0-4.40% w/v), and the osteogenic potential of entrapped human mesenchymal stem cells (MSC) was examined over 14 days. Physical properties including gelation time, compressive modulus and fiber diameter were dependent upon NaCl content, with gels containing 2.60% NaCl possessing compressive moduli threefold higher than gels without NaCl. Alkaline phosphatase activity was highest for MSC entrapped in gels containing 2.15-2.60% NaCl after 14 days, and all gels exhibited increased calcium incorporation over the culture period. These data confirm that varying the salt concentration of the pre-gel solution can modulate the material properties of fibrin constructs without additional fibrinogen or thrombin, thereby offering a new approach for generating improved cell transplantation vehicles for use in bone tissue regeneration.

  8. Impaired thrombin generation and fibrin clot formation in patients with dilutional coagulopathy during major surgery.

    NARCIS (Netherlands)

    Schols, S.E.; Lance, M.D.; Feijge, M.A.; Damoiseaux, J.; Marcus, M.A.; Hamulyak, K.; Cate, H. ten; Heemskerk, J.W.M.; Pampus, E.C.M. van

    2010-01-01

    Patients subjected to haemodilution during surgery are at increased risk of bleeding. We hypothesised that, in the acquired dilutional coagulopathy, insufficient haemostasis is due to either insufficient thrombin generation or insufficient fibrin clot formation. In tissue factor-activated plasmas fr

  9. Platelet-Rich Fibrin Promotes Periodontal Regeneration and Enhances Alveolar Bone Augmentation

    Directory of Open Access Journals (Sweden)

    Qi Li

    2013-01-01

    Full Text Available In the present study we have determined the suitability of platelet-rich fibrin (PRF as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

  10. Platelet-rich fibrin promotes periodontal regeneration and enhances alveolar bone augmentation.

    Science.gov (United States)

    Li, Qi; Pan, Shuang; Dangaria, Smit J; Gopinathan, Gokul; Kolokythas, Antonia; Chu, Shunli; Geng, Yajun; Zhou, Yanmin; Luan, Xianghong

    2013-01-01

    In the present study we have determined the suitability of platelet-rich fibrin (PRF) as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

  11. Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering.

    NARCIS (Netherlands)

    Gassling, V.; Douglas, T.E.L.; Warnke, P.H.; Acil, Y.; Wiltfang, J.; Becker, S.T.

    2010-01-01

    OBJECTIVES: Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the

  12. Bioreactor Conditioning for Accelerated Remodeling of Fibrin-Based Tissue Engineered Heart Valves

    Science.gov (United States)

    Schmidt, Jillian Beth

    Fibrin is a promising scaffold material for tissue engineered heart valves, as it is completely biological, allows for engineered matrix alignment, and is able to be degraded and replaced with collagen by entrapped cells. However, the initial fibrin matrix is mechanically weak, and extensive in vitro culture is required to create valves with sufficient mechanical strength and stiffness for in vivo function. Culture in bioreactor systems, which provide cyclic stretching and enhance nutrient transport, has been shown to increase collagen production by cells entrapped in a fibrin scaffold, accelerating strengthening of the tissue and reducing the required culture time. In the present work, steps were taken to improve bioreactor culture conditions with the goal of accelerating collagen production in fibrin-based tissue engineered heart valves using two approaches: (i) optimizing the cyclic stretching protocol and (ii) developing a novel bioreactor system that permits transmural and lumenal flow of culture medium for improved nutrient transport. The results indicated that incrementally increasing strain amplitude cyclic stretching with small, frequent increments in strain amplitude was optimal for collagen production in our system. In addition, proof of concept studies were performed in the novel bioreactor system and increased cellularity and collagen deposition near the lumenal surface of the tissue were observed.

  13. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin

    Science.gov (United States)

    Shams Asenjan, K.; Dehdilani, N.; Parsa, H.

    2017-01-01

    Objectives Mesenchymal stem cells have the ability to differentiate into various cell types, and thus have emerged as promising alternatives to chondrocytes in cell-based cartilage repair methods. The aim of this experimental study was to investigate the effect of bone marrow derived mesenchymal stem cells combined with platelet rich fibrin on osteochondral defect repair and articular cartilage regeneration in a canine model. Methods Osteochondral defects were created on the medial femoral condyles of 12 adult male mixed breed dogs. They were either treated with stem cells seeded on platelet rich fibrin or left empty. Macroscopic and histological evaluation of the repair tissue was conducted after four, 16 and 24 weeks using the International Cartilage Repair Society macroscopic and the O’Driscoll histological grading systems. Results were reported as mean and standard deviation (sd) and compared at different time points between the two groups using the Mann-Whitney U test, with a value regeneration. It is postulated that platelet rich fibrin creates a suitable environment for proliferation and differentiation of stem cells by releasing endogenous growth factors resulting in creation of a hyaline-like reparative tissue. Cite this article: D. Kazemi, K. Shams Asenjan, N. Dehdilani, H. Parsa. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin: Macroscopic and histological assessments. Bone Joint Res 2017;6:98–107. DOI: 10.1302/2046-3758.62.BJR-2016-0188.R1. PMID:28235767

  14. Fibrin matrices enhance the transplant and efficacy of cytotoxic stem cell therapy for post-surgical cancer.

    Science.gov (United States)

    Bagó, Juli R; Pegna, Guillaume J; Okolie, Onyi; Hingtgen, Shawn D

    2016-04-01

    Tumor-homing cytotoxic stem cell (SC) therapy is a promising new approach for treating the incurable brain cancer glioblastoma (GBM). However, problems of retaining cytotoxic SCs within the post-surgical GBM resection cavity are likely to significantly limit the clinical utility of this strategy. Here, we describe a new fibrin-based transplant approach capable of increasing cytotoxic SC retention and persistence within the resection cavity, yet remaining permissive to tumoritropic migration. This fibrin-based transplant can effectively treat both solid and post-surgical human GBM in mice. Using our murine model of image-guided model of GBM resection, we discovered that suspending human mesenchymal stem cells (hMSCS) in a fibrin matrix increased initial retention in the surgical resection cavity 2-fold and prolonged persistence in the cavity 3-fold compared to conventional delivery strategies. Time-lapse motion analysis revealed that cytotoxic hMSCs in the fibrin matrix remain tumoritropic, rapidly migrating from the fibrin matrix to co-localize with cultured human GBM cells. We encapsulated hMSCs releasing the cytotoxic agent TRAIL (hMSC-sTR) in fibrin, and found hMSC-sTR/fibrin therapy reduced the viability of multiple 3-D human GBM spheroids and regressed established human GBM xenografts 3-fold in 11 days. Mimicking clinical therapy of surgically resected GBM, intra-cavity seeding of therapeutic hMSC-sTR encapsulated in fibrin reduced post-surgical GBM volumes 6-fold, increased time to recurrence 4-fold, and prolonged median survival from 15 to 36 days compared to control-treated animals. Fibrin-based SC therapy could represent a clinically compatible, viable treatment to suppress recurrence of post-surgical GBM and other lethal cancer types.

  15. Fibrin-mediated integrin signaling plays a critical role in hepatic regeneration after partial hepatectomy in mice

    Science.gov (United States)

    Beier, Juliane I.; Guo, Luping; Joshi-Barve, Swati; Ritzenthaler, Jeffrey D.; Roman, Jesse; Arteel, Gavin E.

    2016-01-01

    Background The regenerative capacity of the liver is critical for proper responses to injury. Fibrin extracellular matrix (ECM) deposition is a common response to insult and contributes to inflammatory liver injury. However, the role of this matrix in hepatic regeneration has not been determined. Objective The purpose of this study was first to determine the role of fibrin ECM in hepatic regeneration followed by the role of the fibrin-binding αvβ3 integrin in mediating this effect. Material and methods C57BI/6J (WT) or PAI-1 knockout (KO) mice underwent 70% partial hepatectomy (PHx); plasma and histologic indices of regeneration were determined, as well as expression of key genes involved in hepatic regeneration. Results PHx promoted transient fibrin deposition by activating coagulation and concomitantly decreasing fibrinolysis. Inhibiting fibrin deposition, either by blocking thrombin (hirudin) in WT mice or by knocking out PAI-1, was associated with a decrease in hepatocyte proliferation after PHx. This strongly suggested a role for fibrin ECM in liver regeneration. To investigate if αvβ3 integrin mediates this action, we tested the effects of the anti-αvβ3 cyclic peptide RGDfV in animals after PHx. As was observed with inhibition of fibrin deposition, competitive inhibition of αvβ3 integrin delayed regeneration after PHx, while not affecting fibrin deposition. These effects of RGDfV correlated with impaired angiogenesis and STAT3 signaling, as well as transient endothelial dysfunction. In conclusion, these data suggest that αvβ3 integrin plays an important role in coordinating hepatocyte division during liver regeneration after PHx via crosstalk with fibrin ECM. PMID:27493116

  16. Comparison of Clinical Success of Applying a Kind of Fissure Sealant on the Lower Permanent Molar Teeth in Dry and Wet Conditions

    Directory of Open Access Journals (Sweden)

    Tahereh Eskandarian

    2015-09-01

    Full Text Available Statement of the Problem: Fissure sealant therapy is among the most effective methods of preventing dental caries. However, it is lengthy and isolation of the teeth is difficult in this procedure especially in young children. Using new hydrophilic fissure sealant may reduce such problems. Purpose: This study aimed to evaluate the clinical success of a hydrophilic fissure sealant on the lower permanent mo-lar teeth in dry and wet conditions. Materials and Method: This clinical trial assessed 31 patients (mean age 8.13±1.77 years who needed fissure sealant therapy on their first or second mandibular permanent molar. Having performed dental prophylaxis, the teeth were etched and rinsed. Then one of the two was randomly selected and sealed with smartseal & loc in isolated and dry con-ditions; while, the other was wetted on the etched enamel by using a saliva-contaminated micro brush, and was then sealed with the same fissure as the first tooth. Six and 12 months later, two independent observers examined the clinical success of sealant through checking the marginal integrity, marginal discoloration, and anatomical form. Data were analyzed by using SPSS software, version 16. The bivariate Chi-square and Exact Fisher tests were used to compare the clinical success of the two treatment methods. Results: There was a high interpersonal reliability between the two examiners (K= 0.713. After 12 months, 90.3% clinical success was observed in dry conditions and 83.9% in wet conditions for smartseal & loc; however, the differ-ence was not statistically significant (p= 0.0707. Conclusion: According to the results of this study, it seems that using new hydrophilic fissure sealant can reduce tech-nical sensitivities and consequently decreases the apprehensions on saliva contamination of etched enamel during treatment procedures.

  17. Platelet-rich fibrin-induced bone marrow mesenchymal stem cell differentiation into osteoblast-like cells and neural cells

    Institute of Scientific and Technical Information of China (English)

    Qi Li; Yajun Geng; Lei Lu; Tingting Yang; Mingrui Zhang; Yanmin Zhou

    2011-01-01

    Bone marrow mesenchymal stem cells were allowed to develop for 14 days in a platelet-rich fibrin environment. Results demonstrated that platelet-rich fibrin significantly promoted bone marrow mesenchymal stem cell proliferation. In addition, there was a dose-dependent increase in Runt-related transcription factor-2 and bone morphogenetic protein-2 mRNA expression, as well as neuron-specific enolase and glial acidic protein. Results showed that platelet-rich fibrin promoted bone marrow mesenchymal stem cell proliferation and differentiation of osteoblastlike cells and neural cells in a dose-dependent manner.

  18. Immunohistochemical evaluation of tissue factor, fibrin/fibrinogen and D-dimers in canine gliomas.

    Science.gov (United States)

    de la Fuente, Cristian; Pumarola, Martí; Blasco, Ester; Fernández, Francisco; Viu, Judit; Añor, Sònia

    2014-06-01

    In human gliomas, tissue factor (TF) is overexpressed, associated with the grade of malignancy and influences tumour biology. Intra-tumoural fibrin/fibrinogen deposition and activation of the fibrinolytic system also play a role in tumour cell proliferation and angiogenesis. The first aim of the present study was to investigate TF expression and the presence of fibrin/fibrinogen and D-dimers in canine glioma biopsies, graded according to the World Health Organization (WHO) classification of tumours of the central nervous system. The second aim was to investigate the occurrence of intravascular thrombosis (IVT) in canine gliomas, as a potential histological marker of glioma type or grade of malignancy. An immunohistochemical study using antibodies against TF, fibrin/fibrinogen and D-dimers was performed with 24 glioma samples, including 15 oligodendrogliomas, 6 astrocytomas and 3 mixed gliomas. Immunohistochemical data were statistically analysed to determine whether there was any relationship between glioma type and grade of malignancy. All gliomas were moderate to strongly positive for TF and the staining score was significantly higher (P = 0.04) in high-grade (III or IV) than in low-grade (II) gliomas. Intra-tumoural fibrin/fibrinogen deposition was detected in all tumour biopsies assessed, and D-dimers were detected in 17/24 gliomas. IVT was a frequent finding, but was not linked to a specific glioma type or malignancy grade. TF expression, fibrin/fibrinogen deposition, extravascular fibrinolytic system activation and IVT occur in canine gliomas. Canine glioma might be a suitable model for studying coagulation and fibrinolysis as potential therapeutic targets for human gliomas.

  19. SOLID RADIOACTIVE WASTE STORAGE TECHNOLOGIES: PERFORMANCE OF A POLYMER SEALANT COATING IN AN ARCTIC MARINE ENVIRONMENT

    Energy Technology Data Exchange (ETDEWEB)

    COWGILL,M.G.; MOSKOWITZ,P.D.; CHERNAENKO,L.M.; NAZARIAN,A.; GRIFFITH,A.; DIASHEV,A.; ENGOY,T.

    2000-06-14

    This first project, under the auspices of the Arctic Military Environmental Cooperation (AMEC) forum, Project 1.4-1 Solid Radioactive Waste Storage Technologies, successfully demonstrated the feasibility of using a polymer-based coating to seal concrete and steel surfaces from permanent radioactive contamination in an Arctic marine environment. A mobile, self-sufficient spraying device, was developed to specifications provided by the Russian Ministry of Defence Northern Navy and was deployed at the RTP Atomflot site, Murmansk, Russia. Demonstration coatings of Polibrid 705 were applied to concrete surfaces exposed to conditions ranging from indoor pedestrian usage to heavy vehicle passage and container handling in a loading bay. A large steel container was also coated with the polymer, filled with solid radwaste, sealed, and left out of doors and exposed to the full 12 month Arctic weather cycle. The field tests were accompanied by a series of laboratory qualification tests carried out at the research laboratory of ICC Nuclide in St. Petersburg. During the 12-month field tests, the sealant coating showed little sign of degradation except for a few chips and gouge marks on the loading bay surface that were readily repaired. Contamination resulting from radwaste handling was easily removed and the surface was not degraded by contact with the decontamination agents. In the laboratory testing, Polibrid 705 met all the Russian qualification requirements with the exception of flammability. In this last instance, it was decided to restrict application of the coating to land-based facilities. The Russian technical experts from the Ministry of Defence quickly familiarized themselves with the equipment and were able to identify several areas of potential improvement as deployment of the equipment progressed. The prime among these was the desirability of extending the range of the equipment through enlarged gasoline tanks (to permit extended operational times) and longer

  20. Effect of irradiation times on the polymerization depth of contemporary fissure sealants with different opacities

    Directory of Open Access Journals (Sweden)

    Boniek Castillo Dutra Borges

    2011-04-01

    Full Text Available The aim of this study was to evaluate the depth of curing of 10 contemporary blue light-activated dental flowable materials at several opacities, influenced by different irradiation times using FT-IR spectroscopy. Fifty-five specimens (n = 5 with a 5-mm diameter and 1-mm thickness of translucent (Opallis Flow T, yellowed (Master Flow A2; Opallis Flow A2; Natural Flow A2; Fluroshield Yellowed, and opaque materials (Master Flow OA2; Natural Flow O; Opallis Flow OA3.5; Opallis Flow OP; Fluroshield White were obtained at six curing times (10s, 20s, 30s, 40s, 50s, and 60s using a high-intensity LED (Coltolux, Coltène/Whaledent. The degree of conversion (DC (% was obtained using the Nexus 470 FTIR Spectrometer (Nicolet Instruments, USA. The FTIR-ATR spectra for uncured and cured samples were analyzed using a ZnSe crystal. The top and bottom surfaces of the cured specimens were analyzed to obtain the depth of curing. Two-way ANOVA was used to analyze the data. The highest curing depth was obtained by Natural Flow OA2, while the lowest was shown by Master Flow OA2. The shortest curing time generated similar depths of cure in comparison with the most extensive for Opallis Flow A2 and Fluroshield Yellowed. Therefore, depth of curing, influenced by the irradiation time, was dependent on the materials. Using the Natural Flow OA2 opaque sealant and the 10-s curing time for Opallis Flow A2 and Fluroshield Yellowed may represent alternative approaches to sealing tooth fissures.

  1. Finite Element Analysis of Magnetic Microparticle Induced Strain on a Fibrin Matrix due to the Influence of an Electromagnetic Field

    CERN Document Server

    Averett, Rodney D; Scogin, Tyler; Walker, Mitchell L R

    2016-01-01

    Blood clots occur in the human body when they are required to prevent bleeding. In pathological states such as diabetes and sickle cell disease, blood clots can also form undesirably due to hypercoagulable plasma conditions. With the continued effort in developing fibrin therapies for potential life-saving solutions, more mechanical modeling is needed to understand the properties of fibrin structures with inclusions. In this study, a fibrin matrix embedded with magnetic micro particles was subjected to a magnetic field to determine the plastic deformation of the clot. Using finite element analysis, we estimate the magnetic force from an electromagnet at a sample space located approximately 3 cm away from the coil center. This electromagnetic force along with gravity is applied on a fibrin sub model to calculate the stresses and displacements. Initial analyses show the forces are not sufficient to create fibrinolysis and hence we extended the study using parametric sweep analysis and redesign the coil paramete...

  2. In-vitro-Studie zum Einfluss von Fibrin in Knorpelkonstrukten auf der Basis von PGA-Vliesstoffen

    DEFF Research Database (Denmark)

    Schmal, H; Mehlhorn, A T; Kurze, C

    2008-01-01

    and cultured using a serum-free technique. The cells were seeded on PGA-91 scaffolds with and without an additional fibrin component; the constructs were cultured for 2 weeks in vitro. Besides cell viability, DNA content, pH, aggrecan production, mRNA expression of aggrecan, and collagen types I and II were...... remained within a physiologic range. The Alcian blue staining of the constructs showed the homogeneous cell distribution and a cell-associated proteoglycan production. Aggrecan concentration in the supernatants of fibrin-containing constructs was significantly lower compared to fibrin-free grafts (-24......BACKGROUND: The matrix component in autologous chondrocyte implantation plays an important role. In this study the influence of an additional fibrin component in cartilage constructs based on polyglycolide polymers (PGA) was investigated. METHODS: Human chondrocytes of femoral heads were isolated...

  3. [Experience in fibrin glue application for the treatment of non-formed fistulas of the gastrointestinal tract].

    Science.gov (United States)

    Chernousov, A F; Khorobrykh, T V; Ishchenko, O V

    2006-01-01

    Fibrin glue was used for conservative closure of non-formed fistulas of the gastrointestinal tract in 38 patients. The variant of fibrin glue produced with cryoprecipitation (concentration of coagulant protein not less than 60 g/l) was applied as reparation stimulator. Fibrin glue has been used for closure of 8 duodenal and 4 gastric fistulas with chime loss to 1.5 l per day. Surgeries for palliative arrest of the intestinal loop with a fistula and classical obturators were not performed. The fistulas have been closed in 36 patients, 3 patients died (only 1 of them due to fistula). Fibrin glue reduced surgical aggression and improved treatment results in critically ill patients.

  4. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features.

    Science.gov (United States)

    Dohan, David M; Choukroun, Joseph; Diss, Antoine; Dohan, Steve L; Dohan, Anthony J J; Mouhyi, Jaafar; Gogly, Bruno

    2006-03-01

    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this second article, we investigate the platelet-associated features of this biomaterial. During PRF processing by centrifugation, platelets are activated and their massive degranulation implies a very significant cytokine release. Concentrated platelet-rich plasma platelet cytokines have already been quantified in many technologic configurations. To carry out a comparative study, we therefore undertook to quantify PDGF-BB, TGFbeta-1, and IGF-I within PPP (platelet-poor plasma) supernatant and PRF clot exudate serum. These initial analyses revealed that slow fibrin polymerization during PRF processing leads to the intrinsic incorporation of platelet cytokines and glycanic chains in the fibrin meshes. This result would imply that PRF, unlike the other platelet concentrates, would be able to progressively release cytokines during fibrin matrix remodeling; such a mechanism might explain the clinically observed healing properties of PRF.

  5. Evaluación de fibrinógeno plasmático en pacientes con enfermedad periodontal

    OpenAIRE

    Milla-Torres, Daniela; García-Linares, Sixto

    2014-01-01

    Objetivo: Comprobar si la presencia de enfermedad periodontal aumenta los niveles de fibrinógeno, para sumar evidencia en la relación de causalidad entre las enfermedades periodontal y cardiovascular. Material y métodos: Se evaluaron un total de 40 individuos, divididos en 4 grupos: control (n=10), enfermedad periodontal localizada (n=15) y enfermedad periodontal generalizada (n=15). Resultados: Los pacientes con enfermedad periodontal presentan niveles de fibrinógeno significativamente mayor...

  6. Comparison of viability of adipose-derived Mesenchymal stem cells on agarose and fibrin glue scaffolds

    Directory of Open Access Journals (Sweden)

    Farzaneh Tafvizi

    2015-06-01

    Full Text Available Background & aim: Utilizing tissue engineering techniques and designing similar structures of the damaged tissues require the use of tools such as scaffolds, cells, and bioactive molecules in vitro. Meanwhile, appropriate cell cultures with the ability to divide and differentiate on the natural scaffolds lacking features like immunogenicity and tumorgenesis is particularly important. Adipose tissue has attracted researchers’ attention due to its abundance of mesenchymal stem cells and its availability through a liposuction. The purpose of the present study was to investigate the reproducibility and viability of the adipose-derived stem cells on natural scaffolds of fibrin glue and agarose. Methods: In the present experimental study, the isolation and identification of the mesenchymal stem cells was performed on tissue obtained from liposuction. The tissues were extensively washed with PBS and were digested with collagenase I, then the mesenchymal stem cells were isolated. The cells were cultured in RPMI medium supplemented with antibiotic. Subsequently, the expression of cell surface markers including CD34, CD44, CD90, and CD105 were analyzed by flow cytometry to confirm the mesenchymal cells. After preparing fibrin glue and agarose scaffolds, the viability and proliferation of the adipose tissue-derived mesenchymal stem cells were examined at the period of 24, 48, and 72 hours by MTT and ELISA assays. The obtained results were analyzed by SPSS ver.19. Results: The results of adipose tissue-derived mesenchymal stem cells culture on the fibrin glue and agarose scaffolds indicated that cell viability on fibrin glue and agarose scaffold were 68.22% and 89.75% in 24 hrs, 64.04% and 66.97% in 48 hours, 222.87% and 1089.68% in 72 hours respectively. Significant proliferation and viability cells on a synthesized agarose scaffold were seen compared to the fibrin glue scaffold after 72 hrs. The viability of the cells significantly increased on the

  7. Experimental closure of gunshot wounds by fibrin glue with antibiotics in pigs

    Directory of Open Access Journals (Sweden)

    Đenić Nebojša

    2015-01-01

    Full Text Available Background/Aim. Gunshot wounds caused by the automatic rifle M70AB2 (AK-47 7.62 mm, after the primary surgical management, were closed with delayed primary suture during the next four to seven days. This period coincides with the fibroblastic phase of wound healing. Fibrin glue is used as a local hemostatic and as a matrix for the local dosed release of antibiotics. Antibiotics addition to fibrin glue resulted in continuous diffusion into the surrounding next 4 to 7 days. The aim of this study was to create the preconditions for gunshot wounds closing without complications by the application of fibrin glue with antibiotics 24 h after primary surgical treatment. Methods. A total of 14 pigs were wounded in the gluteofemoral region by the bullet M67, initial velocity of 720 m/s. All wounded animals were surgically treated according to the principles of the warsurgery doctrine. Seven wounds were closed with primary delayed suture four days after the primary surgical treatment (traditional approach. Fibrin glue with antibiotics was introduced in seven wounds during the primary surgical treatment and primary delayed suture was done after 24 h. The macroscopic appearance and the clinical assessment of the wound were done during the primary surgical treatment and during its revision after 24 h, as well as histopathological findings at the days 4 and 7 after wounding. Results. Gunshot wounds caused by the automatic rifle M70AB2 (AK-47 7.62 mm, and treated with fibrin glue with antibiotics after primary surgical management, were closed with primary delayed suture after 24 h. In further wound evolution there were no complications. Conclusion. Uncomplicated soft-tissue wounds caused by an automatic M70AB2 rifle may be closed primarily with delayed suture without the risk of developing complications if on revision, 24 h after primary surgery, there were no present necrotic tissues, hematoma, and any signs of infection when fibrin glue with antibiotics

  8. Removal of organic debris with Er:YAG laser irradiation and microleakage of fissures sealants in vitro.

    Science.gov (United States)

    Hossain, Mozammal; Yamada, Yoshishige; Masuda-Murakami, Yoshiko; Nakamura, Yukio

    2012-09-01

    The current study was conducted to improve fissure sealing by pre-treatment with Er:YAG laser irradiation in order to remove organic debris. The surface morphology, surface roughness of fissure cavities, and the degree of microleakage after laser treatment were compared with those after bristle brush treatment in vitro. Sixty extracted human teeth were used in this study. The teeth were randomly divided into two groups of 30 each. Artificial fissures were prepared in all teeth into which artificial organic debris was placed. The debris in 30 teeth of one group was removed by means of Er:YAG laser system and the remaining 30 teeth were cleaned using a bristle brush with prophylaxis paste. Surface morphology and surface roughness of were analyzed in ten samples from each group by color laser three-dimensional (3D) microscopy and by scanning electron microscopic examination. The remaining samples were then filled with sealant and subjected to a microleakage test under thermocycling. Statistical analysis was performed using the Mann-Whitney U test; a value of p Er:YAG laser treatment, whereas some fissures were not cleaned by bristle brush. However, microleakage test of both laser and etched brush methods showed similar results. Laser technique might facilitate good adaptation of resin sealant to enamel, because of an increase in surface roughness and favorable surface characteristics.

  9. Atraumatic restorative treatment - glass ionomer sealants survival after a postgraduate training program in Ecuador: 2-year follow-up

    Directory of Open Access Journals (Sweden)

    Edisson Lopez Rios

    2015-02-01

    Full Text Available This study aimed to evaluate the survival of atraumatic restorative treatment high-viscosity glass ionomer sealants (ART-hvGIS and its relationship with carious lesions incidence in underserved communities of Ecuador. A total of 483 first permanent molars with ART-hvGIS were included after treatment, in which fifteen students of the First Preventive Dentistry Post Graduate Program of Universidad Central del Ecuador assisted 176 schoolchildren, from 5 to 12 year-old. All the students had previously participated in a training course in ART approach. After one and two years follow-up, schoolchildren were reevaluated in relation to sealants retention and new carious lesions development. After first year of evaluation ART-hvGIS retention rate was about 30% and after second year 20%. Incidence of carious lesions was 4.0 and 3.4% after first and second periods, respectively. Although there was an extensive ART-hvGIS loss, carious lesions incidence was low in both the periods of evaluation. ART-hvGIS might be an important key to prevent tooth decay in underserved communities.

  10. Influence of surface sealant on the translucency of composite resin: effect of immersion time and immersion media

    Directory of Open Access Journals (Sweden)

    Patrícia Petromilli Nordi Sasso Garcia

    2008-06-01

    Full Text Available This study evaluated the effect of surface sealant on the translucency of composite resin immersed in different solutions. The study involved the following materials: Charisma, Fortify and coffee, Coca-Cola®, tea and artificial saliva as solutions. Sixty-four specimens (n = 8 were manufactured and immersed in artificial saliva at 37 ± 1 °C. Samples were immersed in the solutions for three times a day and re-immersed in artificial saliva until the translucency readings. The measurements were carried out at nine times: T1 - 24 hours after specimen preparation, T2 - 24 hours after immersion in the solutions, T3 - 48 hours and T4 to T9 - 7, 14, 21, 30, 60 and 90 days, respectively, after immersion. The translucency values were measured using a JOUAN device. The results were subjected to ANOVA and Tukey's test at 5%. The surface sealant was not able to protect the composite resin against staining, the coffee showed the strongest staining action, followed by tea and regarding immersion time, a significant alteration was noted in the translucency of composite resin after 21 days.

  11. The effect of using different rinsing angles on the micro-tensile bond strength of the sealant to the etched enamel

    Science.gov (United States)

    Afshar, Hossein; Nakhjavani, Yahya Baradaran; Ahmadi, Rahil

    2014-01-01

    Background: Attempts to enhance bond strength of the sealant have been among the most important sides of dental research. Aim: The purpose of the present study was to evaluate the effect of using different rinsing angles on the micro-tensile bond strength of the sealant to the etched enamel. Settings and Design: Experimental study. Materials and Methods: Sixty first-premolars were randomly assigned to six groups based on the rinsing angle applied (15°, 30°, 45°, 60°, 75°, and 90°). Following etching and rinsing, a 4-mm height build up of sealant material was created. Bonded specimens were sectioned into sticks (1 × 1 mm), which were subjected to micro-tensile bond strength, testing at a cross-head speed of 0.5 mm/min. Statistical Analysis Used: The data were analyzed by Kolmogorov-Smirnov and post-hoc Tukey test. Results: The tensile bond strength in specimens rinsed at 90° were statistically higher compared to those rinsed at 15° and 30° (P bond strength and retention of the sealant. PMID:24808698

  12. A comparative study to evaluate the effect of fluoride releasing sealant cured by visible light, argon laers, and light emitting diode curing units: An in vitro study

    Directory of Open Access Journals (Sweden)

    Das U

    2009-09-01

    Full Text Available In Pediatric Dentistry, the use of pit and fissure sealant is one of the essential forms of prevention. Pit and fissure caries may be substantially decreased by obliteration of these developmental defects in occlusal, buccal and lingual surfaces with resin based dental sealants. Visible light-curing units, or LCUs, are an integral part of modern adhesive dentistry" They are used to cure resin based composite restoration materials, resin modified glass-ionomer and pit and fissure sealants, as well as to bond orthodontic teeth. Most recently, the argon laser has been approved for initiating the setting reaction with visible light cured-resins. Argon curing of resin materials has been show to enhance the physical properties and degree of polymerization of the resin, while reducing the polymerization time by 75 percent. The study is undertaken considering the inadequate research reports of regarding the comparison of curing ability using argon laser. LEDs and visible light as well as the resistant towards caries activity of the preventive fluoride releasing pit and fissure sealant cured by above mentioned curing units.

  13. Application of litmus paper and fibrin sealant in pancreatic trauma%石蕊试纸和纤维蛋白胶在胰腺外伤手术中的应用

    Institute of Scientific and Technical Information of China (English)

    尚培中; 黄艳平; 周凤桐; 钱俊林; 曹淑珍

    2004-01-01

    胰腺外伤术后胰瘘发生率高达8%~50%,是影响治疗效果及患者预后的重要并发症。近年来,作者在术中应用石蕊试纸和纤维蛋白胶处理胰腺外伤17例,收到较好效果,报告如下。

  14. 纤维蛋白胶在乳腺癌手术中应用的临床分析%Clinical application of fibrin sealant in surgical operation of breast cancer

    Institute of Scientific and Technical Information of China (English)

    余发斌

    2007-01-01

    目的 探讨纤维蛋白胶在乳腺癌根治性术中的应用价值.方法 将69例乳腺癌手术的患者随机分成两组,一组34例使用纤维蛋白胶喷洒创面(使用组),另一组35例不用纤维蛋白胶(对照组),然后统计术后72 h的引流量,延迟拔管数及皮瓣坏死数及住院时间、患侧上肢功能等.结果 使用纤维蛋白胶组术后72 h的引流量平均为102 ml,明显低于对照组的236 ml.使用组延迟拔管3例(5.5%),对照组延迟拔管11例(30.1%),差异具有统计学意义(P<0.01).使用组皮瓣坏死6例(17.4%),对照组皮缘坏死7例(20.0%),P>0.05.结论 乳腺癌改良根治术中使用纤维蛋白胶能减少术后创面渗出,减少皮瓣下积液,缩短术后拔管时间,有利于术后患者早期恢复,但并不能减少术后患者皮瓣坏死率.

  15. Application of fibrin sealant to postoperative enterocutaneous fistulas:a report of 32 cases%纤维蛋白胶封堵法治疗肠外瘘32例报告

    Institute of Scientific and Technical Information of China (English)

    沈贤; 马立业; 方国恩; 华积德

    2006-01-01

    目的 探讨纤维蛋白胶封堵法治疗肠外瘘临床应用价值.方法 2003年2月至2006年2月第二军医大学附属长海医院对32例术后肠外瘘病人应用纤维蛋白胶封堵法治疗肠外瘘,观察其临床疗效.结果 31例获得愈合,封堵开始至愈合平均时间(8.9±3.6)d,平均封堵次数(2.3±1.3)次,其中1次者11例,2次者9例,3次及以上者12例,成功率为96.9%.结论 纤维蛋白胶能有效地促进肠外瘘愈合.

  16. Uso do adesivo de fibrina na anastomose esôfago-esofágica cervical, em cães The use of fibrin adhesive in the esophagus-esophageal cervical anastomosis, in dogs

    Directory of Open Access Journals (Sweden)

    Orlando Ribeiro Prado Filho

    2004-08-01

    Full Text Available OBJETIVO: Estudar os efeitos da associação do adesivo cirúrgico de fibrina à técnica operatória da invaginação submucosa, em anastomoses esofagianas. MÉTODO: Trinta e dois cães submetidos à anastomose esôfago-esofágica foram alocados em dois grupos: I com sutura em doze pontos e II com sutura em quatro pontos e vedação com adesivo de fibrina. Os animais foram avaliados no sétimo e décimo-quarto dias de pós-operatório. Foram analisados: a evolução ponderal, o índice de estenose, a incidência de deiscências e fístulas, a presença de secreções na tela subcutânea, a presença de líquido intersticial, matriz protêica, celularidade, fibroblastos, fibras de colágeno e concentração de hidroxiprolina. RESULTADOS: O índice de estenose foi menor para os animais do grupo I no sétimo dia de observação. Nos animais do grupo II a incidência de deiscências, secreção serosa e purulenta foram signitivamente maiores aos sete e quatorze dias, enquanto a presença de fístulas foi maior no sétimo dia. Quanto à concentração tecidual de hidroxiprolina não houve diferença estatística entre os grupos. Os fibroblastos e fibras de colágeno tiveram presença mais acentuada no grupo II no décimo-quarto dia. Ocorreram quatro óbitos em animais do grupo II. CONCLUSÕES: A anastomose por invaginação submucosa-mucosa com vedação com adesivo de fibrina apresentou piores resultados que a anastomose convencional de doze pontos circunferenciais.BACKGROUND: The aim of this research was to study the effects of the use of fibrin adhesive with the surgical technique of submucosa invagination, in esophageal anastomosis. METHODS: Thirty two dogs operated on for esophageal anastomosis, were distributed in two groups: group I with encircling suture of twelve stitches, and group II with encircling suture of four stitches and fibrin sealant. The anastomosis was evaluated in the 7th and 14th post-operative day. Weight evolution, stenosis

  17. 硅酮密封胶失效研究及其模型的建立%Structural Silicone Sealant Aging Failure Analysis and the Practical Failure Model

    Institute of Scientific and Technical Information of China (English)

    李倩; 夏美霞; 彭浩; 何潇俊

    2016-01-01

    In order to analyze the effect of structural silicone sealants’ compatibility and adhesion to safety and service life of glass curtain, this paper described various measures for solving the aging of structural silicone sealant. Causes of thermal aging and thermal weightlessness are analyzed through total organic silicon polymer(silicone+silicone oil) preliminarily; and the artificial acceleration is utilized for aging to simulate the actual effect of high temperature to failure of silicone sealant. The results showed that, the thermal aging and thermal weightlessness had significantly damage on the tensile strength and adhesive area of silicone sealant. The total amount of organic silicon polymer(silicone+silicone oil) determined the quality and service life of structural silicone sealants. Salt spray and cleaning agent for building had a great influence on high modulus sealant, but it had no effect on the deformation of low modulus sealant. The decay rate of structural silicone sealant increased with the temperature rising and heat treatment time changing, through establishing the model for the practical application in engineering construction.%为了分析硅酮结构密封胶的相容性和粘结性对玻璃幕墙安全性和使用寿命的影响,阐述了各种老化处理对硅酮结构密封胶失效的影响。通过有机硅聚合物总量(硅胶+硅油)来初步分析了热老化-热失重的原因,并通过人工加速老化模拟实际高温对硅酮结构胶失效的影响。结果表明,热老化-热失重对硅酮结构密封胶的拉伸强度和粘结破坏面积影响显著。有机硅聚合物总量(硅胶+硅油)决定了硅酮结构密封胶的质量和使用寿命。盐雾和建筑物清洁剂对高模量胶影响很大,但基本不影响低模量胶的形变能力。通过构建硅酮结构密封胶在工程中的实际应用情况试验模型,硅酮结构密封胶的衰减率随温度升高和高温处理时间增加而增加。

  18. Acute Fibrinous and Organizing Pneumonia Associated With Allogenic Hematopoietic Stem Cell Transplant Successfully Treated With Corticosteroids

    Directory of Open Access Journals (Sweden)

    Lam-Phuong Nguyen DO

    2016-04-01

    Full Text Available Acute fibrinous and organizing pneumonia (AFOP is an extremely rare, relatively new, and distinct histological pattern of acute lung injury characterized predominately by the presence of intra-alveolar fibrin and associated organizing pneumonia. AFOP may be idiopathic or associated with a wide spectrum of clinical conditions. It has a variable clinical presentation from mild respiratory symptoms to that similar to the acute respiratory distress syndrome. Currently there is no consensus on treatment, and corticosteroids previously were of unclear benefit. To date, there are less than 40 cases of AFOP reported in the literature and only one has been linked to hematopoietic stem cell transplantation. Here we report the first case series of 2 patients who developed AFOP following allogenic stem cell transplant that were successfully treated with high-dose corticosteroids.

  19. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering*

    Science.gov (United States)

    Han, Chun-mao; Zhang, Li-ping; Sun, Jin-zhang; Shi, Hai-fei; Zhou, Jie; Gao, Chang-you

    2010-01-01

    To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications. PMID:20593518

  20. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering.

    Science.gov (United States)

    Han, Chun-mao; Zhang, Li-ping; Sun, Jin-zhang; Shi, Hai-fei; Zhou, Jie; Gao, Chang-you

    2010-07-01

    To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.

  1. Acute fibrinous and organising pneumonia: a rare histopathological variant of chemotherapy-induced lung injury.

    Science.gov (United States)

    Gupta, Arjun; Sen, Shiraj; Naina, Harris

    2016-04-06

    Bleomycin-induced lung injury is the most common chemotherapy-associated lung disease, and is linked with several histopathological patterns. Acute fibrinous and organising pneumonia (AFOP) is a relatively new and rare histological pattern of diffuse lung injury. We report the first known case of bleomycin-induced AFOP. A 36-year-old man with metastatic testicular cancer received three cycles of bleomycin, etoposide and cisplatin, before being transitioned to paclitaxel, ifosfamide and cisplatin. He subsequently presented with exertional dyspnoea, cough and pleuritic chest pain. CT of the chest demonstrated bilateral ground glass opacities with peribronchovascular distribution and pulmonary function tests demonstrated a restrictive pattern of lung disease with impaired diffusion. Transbronchial biopsy revealed intra-alveolar fibrin deposits with organising pneumonia, consisting of intraluminal loose connective tissue consistent with AFOP. The patient received high-dose corticosteroids with symptomatic and radiographic improvement. AFOP should be recognised as a histopathological variant of bleomycin-induced lung injury.

  2. Anisotropic mechanical properties of magnetically aligned fibrin gels measured by magnetic resonance elastography.

    Science.gov (United States)

    Namani, Ravi; Wood, Matthew D; Sakiyama-Elbert, Shelly E; Bayly, Philip V

    2009-09-18

    The anisotropic mechanical properties of magnetically aligned fibrin gels were measured by magnetic resonance elastography (MRE) and by a standard mechanical test: unconfined compression. Soft anisotropic biomaterials are notoriously difficult to characterize, especially in vivo. MRE is well-suited for efficient, non-invasive, and non-destructive assessment of shear modulus. Direction-dependent differences in shear modulus were found to be statistically significant for gels polymerized at magnetic fields of 11.7 and 4.7 T compared to control gels. Mechanical anisotropy was greater in the gels polymerized at the higher magnetic field. These observations were consistent with results from unconfined compression tests. Analysis of confocal microscopy images of gels showed measurable alignment of fibrils in gels polymerized at 11.7 T. This study provides direct, quantitative measurements of the anisotropy in mechanical properties that accompanies fibril alignment in fibrin gels.

  3. Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs

    Directory of Open Access Journals (Sweden)

    G. C. Ferraro

    2005-12-01

    Full Text Available The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute phase, facilitating the cicatricial maturation phase. Snake venom derived fibrin glue promotes the healing in dog flexor tendon.

  4. Fibrinous Pericardial Effusion and Valvulitis Secondary to Previous Acute Rheumatic Fever: An Unusual Clinical Presentation

    Directory of Open Access Journals (Sweden)

    Osman Yılmaz

    2014-12-01

    Full Text Available Rheumatic heart disease, a sequela to acute rheumatic fever (ARF, is a major cause of acquired heart disease in children and young adults in developing countries. Valvular disease of variable severity, heart failure, and pericarditis has been observed in patients with rheumatic heart disease. A 12-year-old female patient presented with fever presented for 3 days, continuing for fatigue, exhaustion, and chest pain. Echocardiography revealed a pericardial effusion with a 24-mm-thick fibrin accumulation in the neighborhood of the left ventricle. Review of the patient’s medical history revealed that about 3 months earlier the patient had experienced migrating swelling, erythema, and pain of the ankles and knees that lasted for 1 week. Here, we present an ARF patient with an unusual clinical presentation who thought to have an attack of acute rheumatic fever 3 months earlier and was presented with fibrinous pericardial effusion.

  5. The Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Their Conditioned Media Topically Delivered in Fibrin Glue on Chronic Wound Healing in Rats

    OpenAIRE

    Mehanna, Radwa A.; Iman Nabil; Noha Attia; Bary, Amany A.; Razek, Khalid A.; Ahmed, Tamer A. E.; Fatma Elsayed

    2015-01-01

    Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent a modern approach for management of chronic skin injuries. In this work, we describe BM-MSCs application versus their conditioned media (CM) when delivered topically admixed with fibrin glue to enhance the healing of chronic excisional wounds in rats. Fifty-two adult male rats were classified into four groups after induction of large-sized full-thickness skin wound: control group (CG), fibrin only group (FG), fibrin + MSCs group (...

  6. Revascularization of Immature Necrotic Teeth: Platelet rich Fibrin an Edge over Platelet rich Plasma

    OpenAIRE

    Neelam Mittal; Isha Narang

    2012-01-01

    Introduction: Revascularization is one such entity that has found its clinical application in the field of endodontics for the manage-ment of immature permanent necrotic teeth. The protocols for revascularization of such teeth focus especially on delivery of stem cells and scaffolds in a nonsurgical manner rather than concentrated growth micro molecules.The hypothesis: This article proposes the role of platelet concentrates such as platelet rich fibrin (PRF) and platelet rich plasma (PRP) in ...

  7. Zinc promotes clot stability by accelerating clot formation and modifying fibrin structure.

    Science.gov (United States)

    Henderson, Sara J; Xia, Jing; Wu, Huayin; Stafford, Alan R; Leslie, Beverly A; Fredenburgh, James C; Weitz, David A; Weitz, Jeffrey I

    2016-03-01

    Zinc released from activated platelets binds fibrin(ogen) and attenuates fibrinolysis. Although zinc also affects clot formation, the mechanism and consequences are poorly understood. To address these gaps, the effect of zinc on clot formation and structure was examined in the absence or presence of factor (F) XIII. Zinc accelerated a) plasma clotting by 1.4-fold, b) fibrinogen clotting by 3.5- and 2.3-fold in the absence or presence of FXIII, respectively, c) fragment X clotting by 1.3-fold, and d) polymerisation of fibrin monomers generated with thrombin or batroxobin by 2.5- and 1.8-fold, respectively. Whereas absorbance increased up to 3.3-fold when fibrinogen was clotted in the presence of zinc, absorbance of fragment X clots was unaffected by zinc, consistent with reports that zinc binds to the αC-domain of fibrin(ogen). Scanning electron microscopic analysis revealed a two-fold increase in fibre diameter in the presence of zinc and in permeability studies, zinc increased clot porosity by 30-fold with or without FXIII. Whereas FXIII increased clot stiffness from 128 ± 19 Pa to 415 ± 27 Pa in rheological analyses, zinc reduced clot stiffness by 10- and 8.5-fold in the absence and presence of FXIII, respectively. Clots formed in the presence of zinc were more stable and resisted rupture with or without FXIII. Therefore, zinc accelerates clotting and reduces fibrin clot stiffness in a FXIII-independent manner, suggesting that zinc may work in concert with FXIII to modulate clot strength and stability.

  8. A novel fibrin gel derived from hyaluronic acid-grafted fibrinogen

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Chiung L; Chen, Hui W; Wang, Tzu C; Wang, Yng J, E-mail: wang@ym.edu.tw [Institute of Biomedical Engineering, National Yang Ming University, No. 155, Sec. 2, Li-Nung St., Shih-Pai, Taipei, Taiwan 112 (China)

    2011-04-15

    Fibrinogen is a major plasma protein that forms a three-dimensional fibrin gel upon being activated by thrombin. In this study, we report the synthesis and potential applications of hybrid molecules composed of fibrinogen coupled to the reducing ends of short-chain hyaluronic acids (sHAs) by reductive amination. The grafting of sHAs to fibrinogen was verified by analyzing particle size, zeta potential and gel-electrophoretic mobility of the hybrid molecules. The sHA-fibrinogen hybrid molecules with graft ratios (sHA/fibrinogen) of up to 6.5 retained the ability to form gels in response to thrombin activation. The sHA-fibrin gels were transparent in appearance and exhibited high water content, which were characteristics distinct from those of gels formed by mixtures of sHAs and fibrinogen. The potential applications of the sHA-fibrin gels were evaluated. The sHA-fibrinogen gel with a graft ratio of 3.6 (S3.6F) was examined for its ability to encapsulate and support the differentiation of ATDC5 chondrocyte-like cells. Compared with the fibrinogen-formed gel, cells cultured in the S3.6F gel exhibited increased lacunae formation; moreover, the abundance of cartilaginous extracellular matrix molecules and the expression of chondrocyte marker genes, such as aggrecan, collagen II and Sox9, were also significantly increased. Our data suggest that the three-dimensional gel formed by the sHA-fibrinogen hybrid is a better support than the fibrin gel for chondrogenesis induction.

  9. Acute Fibrinous and Organizing Pneumonia with Myelodysplastic Syndrome: Corticosteroid Monotherapy Led to Successful Ventilator Weaning

    OpenAIRE

    Yamamoto, Mari; Murata, Kengo; Kiriu, Takahiro; Kouzai, Yasuji; Takamori, Mikio

    2016-01-01

    A 62-year-old man with myelodysplastic syndrome (MDS) presented to our hospital with a high fever. Although treatment with broad-spectrum antibiotics was initiated, his respiratory status worsened to the point that he required mechanical ventilation. However, he was successfully treated with a corticosteroid without immunosuppression. Sequential transbronchial lung biopsies revealed abundant fibrin exudate in the alveolar spaces, which was subsequently replaced by fibroblasts, showing that ac...

  10. One-step apexification using platelet rich fibrin matrix and mineral trioxide aggregate apical barrier

    Directory of Open Access Journals (Sweden)

    Anisha Kumar

    2014-01-01

    In one-step apexification using MTA, the technical problem encountered is controlling the overfill or underfill of MTA. The use of a matrix material helps to overcome this shortcoming. Platelet rich fibrin (PRF is an immune platelet concentrate, which can be used as a matrix, it also promotes wound healing and repair. This case report presents a case of one step apexification using MTA as an apical barrier and autologous PRF as an internal matrix.

  11. 填料对聚硫密封剂耐水性能的影响%Effect of filler on water resistance of polysulfide sealants

    Institute of Scientific and Technical Information of China (English)

    刘嘉

    2012-01-01

    This piper is focused on the effect of different fillers on mechanical properties and adhesion properties of polysulfide sealant after soaking in water.After water resistance experiments,it was found that the polysulfide sealants filled with calcined kaolin,fumed silica or wished kaolin had the best water resistance ,the second was as follows :precipitated calcium carbonate.ground calcium carbonate and precipitated silica.The mechanical properties of polysulfide sealant filled with TiO2 or talcum powder changed greatly.The adhesive failure would occur when the polysulfide sealant was filled with silica. And the polysulfide sealant filled with calcined kaolin had the best adhesion property.%选择8种常规无机填料,考查了填料对聚硫密封剂力学性能和粘接性能的影响.耐水试验后发现填充煅烧高岭土、气相SiO2、水洗高岭土的密封剂耐水性能最优,其次是轻质CaCO3、重质CaCO3、沉淀SiO2,填充TiO2和滑石粉的密封剂性能变化较大.二氧化硅填料单独使用对粘接性能影响大,出现脱粘现象,煅烧高岭土为填料时剥离强度变化最小.

  12. Mesenchymal Stromal Cell-Derived PTX3 Promotes Wound Healing via Fibrin Remodeling.

    Science.gov (United States)

    Cappuzzello, Claudia; Doni, Andrea; Dander, Erica; Pasqualini, Fabio; Nebuloni, Manuela; Bottazzi, Barbara; Mantovani, Alberto; Biondi, Andrea; Garlanda, Cecilia; D'Amico, Giovanna

    2016-01-01

    Although mesenchymal stromal cells (MSCs) can promote wound healing in different clinical settings, the underlying mechanism of MSC-mediated tissue repair has yet to be determined. Because a nonredundant role of pentraxin 3 (PTX3) in tissue repair and remodeling has been recently described, here we sought to determine whether MSC-derived PTX3 might play a role in wound healing. Using a murine model of skin repair, we found that Ptx3-deficient (Ptx3(-/-)) MSCs delayed wound closure and reduced granulation tissue formation compared with wt MSCs. At day 2, confocal microscopy revealed a dramatic reduction in green fluorescent protein (GFP)-expressing Ptx3(-/-) MSCs recruited to the wound, where they appeared to be not only poorly organized in bundles but also scattered in the extracellular matrix. These findings were further confirmed by quantitative biochemical analysis of GFP content in wound extracts. Furthermore, Ptx3(-/-) MSC-treated skins displayed increased levels of fibrin and lower levels of D-dimer, suggesting delayed fibrin-rich matrix remodeling compared with control skins. Consistently, both pericellular fibrinolysis and migration through fibrin were found to be severely affected in Ptx3(-/-) MSCs. Overall, our findings identify an essential role of MSC-derived PTX3 in wound repair underscoring the beneficial potential of MSC-based therapy in the management of intractable wounds.

  13. Fibrin glue application in the management of refractory chylous ascites in children.

    Science.gov (United States)

    Zeidan, S; Delarue, A; Rome, A; Roquelaure, B

    2008-04-01

    The purpose of this retrospective review of the charts of 6 children who underwent surgical treatment of chylous ascites refractory to conservative measures between 1993 and 2006 was to evaluate the efficiency of fibrin glue application for control of lymph leakage. Five children had postoperative chylous ascites (neuroblastoma, 4; cystic lymphangioma, 1) and 1 had a congenital malformation. Surgical exploration revealed large areas of diffuse lymphatic leakage in all of the patients. Lymphatic fistula was not identified intraoperatively in any patient. Ingestion of lipophilic dye in a concentrated fatty meal was not helpful in locating a lymph fistula. Absorbable mesh was used in association with glue application in the last 3 patients treated. Control of ascites was achieved immediately in 2 patients and within 3 weeks in 2 patients. Repeat surgery was required in the remaining 2 patients. The mean follow-up time was 4.3 years. One patient died of tumor recurrence 12 months after surgical treatment without relapse of the ascites. Two mild late recurrences were observed at 6 and 11 months after surgery and were managed conservatively. The findings of this study show that fibrin glue application on absorbable mesh after dissection of the leakage zones is easy, safe, and effective. We recommend that surgery with glue application be repeated until control of ascites is achieved. We suggest fibrin glue application as a preventive measure against postoperative chylous ascites.

  14. Pneumococcal association to platelets is mediated by soluble fibrin and supported by thrombospondin-1.

    Science.gov (United States)

    Niemann, Silke; Kehrel, Beate E; Heilmann, Christine; Rennemeier, Claudia; Peters, Georg; Hammerschmidt, Sven

    2009-10-01

    Platelets and coagulation are involved in bacterial colonisation of the host. Streptocococcus pneumoniae (pneumococcus) are important etiologic agents of respiratory tract infections in humans. The formation of pneumococci-platelet associations may facilitate haematogenous dissemination of pneumococci by providing an adhesive surface on damaged endothelium. However, the formation of platelet-pneumococci associations and the factors involved in this process have not been described so far. The formation of platelet-pneumococci associates was analysed and quantified using flow cytometry. Binding of pneumococci to platelets was significantly increased after activation of platelets with thrombin, while platelet activation by ADP or collagen did not promote formation of platelet-pneumococci associates. In addition to be a platelet agonist, thrombin cleaves fibrinogen, which results in the generation of fibrin. The simultaneous formation of fibrin and activation of platelets was shown to be a prerequisite for a high number of platelet-pneumococci associates. Moreover, exogenously added human thrombospondin-1 (TSP-1) significantly enhanced the association of pneumococci with activated platelets. Soluble fibrin and TSP-1 are key co-factors of platelet-pneumococci-association. Similar results were recently demonstrated for S. aureus-platelet adhesion. Consequently, we hypothesise that the described mechanism of platelet-bacteria-association might represent a general and important strategy of Gram-positive bacteria during development of invasive diseases.

  15. Leukocyte accumulation promoting fibrin deposition is mediated in vivo by P-selectin on adherent platelets

    Science.gov (United States)

    Palabrica, Theresa; Lobb, Roy; Furie, Barbara C.; Aronovitz, Mark; Benjamin, Christopher; Hsu, Yen-Ming; Sajer, Susan A.; Furie, Bruce

    1992-10-01

    THE glycoprotein P-selectin is a cell adhesion molecule of stimulated platelets and endothelial cells, which mediates the interaction of these cells with neutrophils and monocytes1,2. It is a membrane component of cell storage granules3-6, and is a member of the selectin family which includes E-selectin and L-selectin7,8. P-selectin recognizes both lineage-specific carbohydrate ligands on monocytes and neutrophils, including the Lewis x antigen, sialic acid, and a protein component9-12. In inflammation and thrombosis, P-selectin may mediate the interaction of leukocytes with platelets bound in the region of tissue injury and with stimulated endothelium1,2. To evaluate the role of P-selectin in platelet-leukocyte adhesion in vivo, the accumulation of leukocytes within an experimental thrombus was explored in an arteriovenous shunt model in baboons13. A Dacron graft implanted within an arteriovenous shunt is thrombogenic, accumulating platelets and fibrin within its lumen. These bound platelets express P-selectin14. Here we show that antibody inhibition of leukocyte binding to P-selectin expressed on platelets immobilized on the graft blocks leukocyte accumulation and inhibits the deposition of fibrin within the thrombus. These results indicate that P-selectin is an important adhesion molecule on platelets, mediating platelet-leukocyte binding in vivo, that the presence of leukocytes in thrombi is mediated by P-selectin, and that these leukocytes promote fibrin deposition.

  16. Molecular mechanisms, thermodynamics, and dissociation kinetics of knob-hole interactions in fibrin

    CERN Document Server

    Kononova, Olga; Zhmurov, Artem; Alekseenko, Andrey; Cheng, Chai-Ho; Agarwal, Silvi; Marx, Kenneth A; Weisel, John W; Barsegov, Valeri

    2015-01-01

    Polymerization of fibrin, the primary structural protein of blood clots and thrombi, occurs through binding of knobs 'A' and 'B' in the central nodule of fibrin monomer to complementary holes 'a' and 'b' in the beta- and gamma-nodules, respectively, of another monomer. We characterized the A:a and B:b knob-hole interactions under varying solution conditions using Molecular Dynamics simulations of the structural models of fibrin(ogen) fragment D complexed with synthetic peptides GPRP (knob 'A' mimetic) and GHRP (knob 'B' mimetic). The strength of A:a and B:b knob-hole complexes was roughly equal, decreasing with pulling force; yet, the dissociation kinetics were sensitive to variations in acidity (pH=5-7) and temperature (T=25-37 C). There were similar structural changes in holes 'a' and 'b' during forced dissociation of the knob-hole complexes: elongation of loop I, stretching of interior region, and translocation of the moveable flap. The disruption of the knob-hole interactions was not an "all-or-none" tran...

  17. Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2.

    Science.gov (United States)

    Li, Qi; Reed, David A; Min, Liu; Gopinathan, Gokul; Li, Steve; Dangaria, Smit J; Li, Leo; Geng, Yajun; Galang, Maria-Therese; Gajendrareddy, Praveen; Zhou, Yanmin; Luan, Xianghong; Diekwisch, Thomas G H

    2014-05-14

    Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF) as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF). LPRF caused a 4.8-fold±0.4-fold elevation in Runt-related transcription factor 2 (Runx2) expression in alveolar bone cells, compared to a 3.6-fold±0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (pfibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

  18. RECONSTRUCTION OF THE SELLAR FLOOR FOLLOWING TRANSSPHE-NOIDAL SURGERY USING GELATIN FOAM AND FIBRIN GLUE

    Institute of Scientific and Technical Information of China (English)

    Jian Yin; Chang-bao Su; Zhi-qin Xu; Xue-wei Xia; Fei Song

    2005-01-01

    Objective To introduce a new principle of sellar reconstruction and to evaluate the effectiveness of absorbable gelatin foam and fibrin glue for sellar reconstruction. Methods A total of 176 consecutive patients who underwent surgery for pituitary adenomas, cysts, chordomas, or subdiaphragmatic craniopharyngiomas in the sella turcica between January 2001 and April 2003 at Peking Union Medical College Hospital were enrolled. Different techniques of sellar closure and indications for each specific condition were retrospectively reviewed. Results Seventy-seven (43.7%) patients developed a visible cerebrospinal fluid (CSF) leakage during surgery. Intra operative CSF leakage were repaired simply with gelatin foam and fibrin glue in 62 (35.2%) patients, and with autologous fat graft and sellar floor reconstruction in 15 (8.5%) patients. Postoperative CSF rhinorrhea occurred only in 1 case. There were no visual deterioration, allergic rhinitis, meningitis, pneumocranium, granulomas, or other complications associated with the reconstruction procedure. Conclusion The procedure of using gelatin foam and fibrin glue and principle of cranial base reconstruction is safe and effective in preventing postoperative complications following transsphenoidal surgery.

  19. Applying psychological theories to evidence-based clinical practice: identifying factors predictive of placing preventive fissure sealants

    Directory of Open Access Journals (Sweden)

    Maclennan Graeme

    2010-04-01

    Full Text Available Abstract Background Psychological models are used to understand and predict behaviour in a wide range of settings, but have not been consistently applied to health professional behaviours, and the contribution of differing theories is not clear. This study explored the usefulness of a range of models to predict an evidence-based behaviour -- the placing of fissure sealants. Methods Measures were collected by postal questionnaire from a random sample of general dental practitioners (GDPs in Scotland. Outcomes were behavioural simulation (scenario decision-making, and behavioural intention. Predictor variables were from the Theory of Planned Behaviour (TPB, Social Cognitive Theory (SCT, Common Sense Self-regulation Model (CS-SRM, Operant Learning Theory (OLT, Implementation Intention (II, Stage Model, and knowledge (a non-theoretical construct. Multiple regression analysis was used to examine the predictive value of each theoretical model individually. Significant constructs from all theories were then entered into a 'cross theory' stepwise regression analysis to investigate their combined predictive value Results Behavioural simulation - theory level variance explained was: TPB 31%; SCT 29%; II 7%; OLT 30%. Neither CS-SRM nor stage explained significant variance. In the cross theory analysis, habit (OLT, timeline acute (CS-SRM, and outcome expectancy (SCT entered the equation, together explaining 38% of the variance. Behavioural intention - theory level variance explained was: TPB 30%; SCT 24%; OLT 58%, CS-SRM 27%. GDPs in the action stage had significantly higher intention to place fissure sealants. In the cross theory analysis, habit (OLT and attitude (TPB entered the equation, together explaining 68% of the variance in intention. Summary The study provides evidence that psychological models can be useful in understanding and predicting clinical behaviour. Taking a theory-based approach enables the creation of a replicable methodology for

  20. Albumin modulates lateral assembly of fibrin polymers: evidence of enhanced fine fibril formation and of unique synergism with fibrinogen

    Energy Technology Data Exchange (ETDEWEB)

    Galanakis, D.K.; Lane, B.P.; Simon, S.R.

    1987-04-21

    The authors identified a new property of human albumin. It enhances formation of fine fibril (or leptofibril) structure during fibrin gelation, and by nephelometric and electron microscopic measurements, this property is independent of and synergistic with that of fibrinogen. They examined fibrin aggregation using physiologic temperatures and pH and albumin:fibrin concentration ratios below those at which the known accelerating effect on fibrin aggregation occurs. An albumin concentration dependent decrease in gel turbidity maxima was consistently demonstrable in buffers containing or lacking (2-5 mM) CaCl/sub 2/. Electron microscopic measurements of cross-sectional fibril widths, performed on sections of glutaraldehyde-fixed gels, disclosed differences between albumin-containing and control gels which were significant. Spin-labeled albumin displayed no change in electron (para) magnetic spin resonance spectral measurements during its inhibition of fibrin, indicating no perturbation on albumin conformation in the vicinities of Cys-34 and of fatty acid binding sites. Certain fibrinogen:albumin ratios designed to induce maximal inhibition yet permit gelation in the presence of either alone prevented gelation of buffer-diluted fibrin monomers. Aliquots from these which were dried and negatively stained on formvar-coated grids disclosed strands of 5-17 nm width, most displaying a 60-250-nm approximate length. The amounts of /sup 131/I-labeled coagulable fibrin which remained soluble in fibrinogen solutions were increased by albumin. They conclude that albumin enhances formation of leptofibril-rich gel domains when other plasma factors favor formation of such structures. Available evidence indicating decreased permeability implies that such gel domains limit efflux rates from the intrathrombus environment and from intra- to extravascular space.

  1. USAGE OF MONOCLONAL ANTIBODIES FOR DETERMINATION OF LOCALIZATION OF ANTIGENIC DETERMINANTS AND FIBRIN POLYMERIZATION SITES WITHIN FIBRINOGEN AND FIBRIN MOLECULES AND THEIR APPLICATION IN TEST--SYSTEMS FOR DIAGNOSTICS AND THE THREAT OF THROMBUS FORMATION

    Directory of Open Access Journals (Sweden)

    E. V. Lugovskoi

    2013-08-01

    Full Text Available It was shown by monoclonal antibodies that B?N-region of fibrin desA molecule (B?1-53 comprises the polymerization site including the peptide bond B?14-15. This site participates in the second stage of fibrin polymerization — lateral association of protofibrils. In the B?15-53 fragment was also found the site called «C», which together with the site «A» participate in the first stage of polymerization — the protofibrils formation. The model of the primary intermolecular interaction of fibrin was designed. It was found by monoclonal antibodies II-4d the site («c» in the N-terminal half of ? chain of the fibrin D-region. This site participates in the protofibrils formation and is complement to site «C» as we assume. We have discovered two neoantigenic determinants. One of these determinants exposes within the coiledcoil fragment B?126-135 of fibrin as a result of fibrinopeptide A splitting off from fibrinogen by thrombin. The structural rearrangements discovered in this site of the fibrin molecule are necessary for the following protofibrils lateral association. The second neoantigenic determinant is localized in the fragment B?134-190 of D-dimer formed after plasmin degradation of fibrin stabilized by FXIIIa. We have obtained the fibrin-specific monoclonal antibodie FnI-3C to the first determinant and D-dimer-specific mAb III-3b to the second one. Three monoclonal antibodies were obtained against the ?C-region of fibrin(ogen molecule. It has been experimentally shown by of one of them that ?C-domains is connected with the fibrinopeptides B in fibrinogen and fibrin desA molecules, but removes from the core of the molecules after fibrinopeptides B splitting off by thrombin. Two other monoclonal antibodies specifically inhibit the fibrin polymerization by blocking two unknown polymerization sites within the ?C-region. The test-systems for the soluble fibrin and D-dimer quantification in human blood plasma were designed on the basis of

  2. 3种窝沟封闭剂微渗漏的实验研究%Experimental Study on Microleakage of Three Different Fissure Sealants

    Institute of Scientific and Technical Information of China (English)

    张海亮; 赵玉宏; 欧晓艳

    2011-01-01

    Objective: To provide experimental evidence by study on microleakage of three different fissure sealants. Methods: Forty human maxillary premolars,caries -free for orthodontic reasons were collected and randomly divided into five groups. Fissures were respectively sealed with conventional pit and fissure sealant (Helioseal F) ? Glass ionomer cement(GC Fuji VII), GC Fuji VII plus saliva contamination, wetbond sealant(Embrace WetBond), Embrace WetBond plus saliva contamination. Six teeth in each group were stained with 50% silver nitrate for 2 hours, then were sectioned for evaluation of microleakage. Two other teeth in each group were used to observe the marginal adaptability of fissure sealants and interface condition by scanning electronic microscope. Results: Without saliva contamination, there were significantly differences concerning the microleakage results among three different sealants. Helioseal F was fully penetrated into the bottom of the fissure by SEM, together with the enamel completely. While with saliva contamination, GC Fuji VII and Embrace WetBond had a poor sealing ability, the interface between sealants and enamel appeared significant micro-gap. Conclusion: The pit and fissure sealant Helioseal F had a excellent marginal adaptation. The new glass ionomer sealant GC Fuji VII and wet -bond sealant Embrace WetBond only needed a technology with lower difficulty, and could achieve a acceptable marginal adaptation, their sealing abilities needed further clinical trials.%目的:比较3种窝沟封闭剂的微渗漏情况,为选择操作技术难度低且具良好边缘密合性的窝沟封闭剂提供实验依据.方法:收集正畸减数的上颌前磨牙40颗,随机分为5组,每组8颗.即:Helioseal F组、GC Fuji Ⅶ组、GC Fuji Ⅶ+唾液污染组、Embrace WetBond组、Embrace WetBond+唾液污染组,每组中6颗用50%硝酸银染料浸润2h,切片于体视显微镜下观察微渗漏情况,余下2颗用扫描电镜观察窝沟封闭剂与牙

  3. Adhesive complex coacervate inspired by the sandcastle worm as a sealant for fetoscopic defects

    Science.gov (United States)

    Kaur, Sarbjit

    Inspired by the Sandcastle Worm, biomimetic of the water-borne adhesive was developed by complex coacervation of the synthetic copolyelectrolytes, mimicking the chemistries of the worm glue. The developed underwater adhesive was designed for sealing fetal membranes after fetoscopic surgery in twin-to-twin transfusion syndrome (TTTS) and sealing neural tissue of a fetus in aminiotic sac for spina bifida condition. Complex coacervate with increased bond strength was created by entrapping polyethylene glycol diacrylate (PEG-dA) monomer within the cross-linked coacervate network. Maximum shear bond strength of ~ 1.2 MPa on aluminum substrates was reached. The monomer-filled coacervate had complex flow behavior, thickening at low shear rates and then thinning suddenly with a 16-fold drop in viscosity at shear rates near 6 s-1. The microscale structure of the complex coacervates resembled a three-dimensional porous network of interconnected tubules. This complex coacervate adhesive was used in vitro studies to mimic the uterine wall-fetal membrane interface using a water column with one end and sealed with human fetal membranes and poultry breast, and a defect was created with an 11 French trocar. The coacervate adhesive in conjunction with the multiphase adhesive was used to seal the defect. The sealant withstood an additional traction of 12 g for 30-60 minutes and turbulence of the water column without leakage of fluid or slippage. The adhesive is nontoxic when in direct contact with human fetal membranes in an organ culture setting. A stable complex coacervate adhesive for long-term use in TTTS and spina bifida application was developed by methacrylating the copolyelectrolytes. The methacrylated coacervate was crosslinked chemically for TTTS and by photopolymerization for spina bifida. Tunable mechanical properties of the adhesive were achieved by varying the methacrylation of the polymers. Varying the amine to phosphate (A/P) ratio in the coacervate formation

  4. Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma.

    Science.gov (United States)

    Ito, Kenji; Yamada, Yoichi; Naiki, Takahito; Ueda, Minoru

    2006-10-01

    This study was undertaken to evaluate the use of tissue-engineered bone as grafting material for alveolar augmentation with simultaneous implant placement. Twelve adult hybrid dogs were used in this study. One month after the extraction of teeth in the mandible region, bone defects on both sides of the mandible were induced using a trephine bar with a diameter of 10 mm. Dog mesenchymal stem cells (dMSCs) were obtained via iliac bone biopsy and cultured for 4 weeks before implantation. After installing the dental implants, the defects were simultaneously implanted with the following graft materials: (i) fibrin, (ii) dMSCs and fibrin (dMSCs/fibrin), (iii) dMSCs, platelet-rich plasma (PRP) and fibrin (dMSCs/PRP/fibrin) and (iv) control (defect only). The implants were assessed by histological and histomorphometric analysis, 2, 4 and 8 weeks after implantation. The implants exhibited varying degrees of bone-implant contact (BIC). The BIC was 17%, 19% and 29% (control), 20%, 22% and 25% (fibrin), 22%, 32% and 42% (dMSCs/fibrin) and 25%, 49% and 53% (dMSCs/PRP/fibrin) after 2, 4 and 8 weeks, respectively. This study suggests that tissue-engineered bone may be of sufficient quality for predictable enhancement of bone regeneration around dental implants when used simultaneous by with implant placement.

  5. Growth factor and proteinase profile of Vivostat® platelet-rich fibrin linked to tissue repair

    DEFF Research Database (Denmark)

    Ågren, Sven Per Magnus; Rasmussen, Karina; Pakkenberg, Bente

    2014-01-01

    BACKGROUND AND OBJECTIVES: Autologous platelet-rich fibrin (PRF(®)) is prepared by the automatic Vivostat(®) system. Conflicting results with Vivostat PRF in acute wound healing prompted us to examine its cellular and biomolecular composition. Specifically, platelets, selected growth factors...... and matrix metalloproteinase (MMP)-9 were quantified using novel analytical methods. MATERIALS AND METHODS: Ten healthy non-thrombocytopenic volunteers donated blood for generation of intermediate fibrin-I and final PRF. Anticoagulated whole blood and serum procured in parallel served as baseline controls....... Leucocyte, erythrocyte and platelet counts in whole blood and fibrin-I were determined by automated haematology analyser. Platelet concentration in PRF was quantified manually by stereologic analysis of Giemsa-stained tissue sections, and the total content of five growth factors and MMP-9 by enzyme...

  6. A HYBRID SCAFFOLD OF POLY(LACTIDE-CO-GLYCOLIDE) SPONGE FILLED WITH FIBRIN GEL FOR CARTILAGE TISSUE ENGINEERING

    Institute of Scientific and Technical Information of China (English)

    Wei Wang; Dan Li; Mei-cong Wang; Yang-lin Li; Chang-you Gao

    2011-01-01

    The poly(lactide-co-glycolide) (PLGA) sponge fabricated by a gelatin porogen leaching method was filled with fibrin gel to obtain a hybrid scaffold for chondrocytes culture in vitro. The fibrin gel evenly distributed in the hybrid scaffold with visible fibrinogen fibers after drying. In vitro culture it was found that in the hybrid scaffold the chondrocytes distributed more evenly and kept a round morphology as that in the normal cartilage. Although the chondrocytes seeded in the control PLGA sponges showed similar proliferation behavior with that in the hybrid scaffolds, they were remarkably elongated, forming a fibroblast-like morphology. Moreover, a larger amount of glycosaminoglycans was secreted in the hybrid scaffolds than that in the PLGA sponges after in vitro culture of chondrocytes for 4 weeks. The results suggest that the fibrin/PLGA hybrid scaffold may be favorably applied for cartilage tissue engineering.

  7. Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium

    Science.gov (United States)

    Li, Jun; Zhu, Kai; Yang, Shan; Wang, Yulin; Guo, Changfa; Yin, Kanhua; Wang, Chunsheng

    2015-01-01

    Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothelial cells and cardiomyocyte-like differentiation of BMSCs in vitro. Four weeks after transplantation of fibrin patch loaded with gene-modified BMSCs, IGF-1 overexpression could successfully promote angiogenesis, inhibit remodeling, increase grafted cell survival and reduce apoptosis. In conclusion, the integrated strategy, which combined fibrin patch with IGF-1 gene modified BMSCs, could promote the histological cardiac repair for a clinically relevant porcine model of I/R myocardium injury. PMID:25767192

  8. KEBERHASILAN PENETRASI BAHAN PIT & FISSURE SEALANT KE DALAM FISURA MENGGUNAKAN SYRINGE BLUE MICRO TIPS DAN SYRINGE WHITE MINI BRUSH TIPS (LAPORAN PENELITIAN

    Directory of Open Access Journals (Sweden)

    Avy Permata Sari

    2015-07-01

    Full Text Available The aim of this study was to know the success and differences of pit & fissure sealant materal penetration of composite resin type (UltraSeal XT Plus into fissure by using syringe blue micro tips and syringe white mini brush tips in fissure closure efforts for caries prevention of permanent teeth. The study was conducted to 30 maxillary first premolar, using syring blue micro tips, and 30 maxilary first premolar using syringw white mini brush tips. The samples, then, were made smears and observed by optic microscope with 300 times magnification. The result of this stuy showed that mean of pit & fissure sealant material penetration of composite resin type into fissure by using blue micro tips was (67.93 ± 13.09%. By using syringe white mini brush tips was (92.96 ± 6.18%. With t-test, it showed a significant difference (t = 9.84, p<0,01.

  9. Synthesis of new water expandable waterproofing sealant and its application%新型水膨胀防水密封胶的合成与应用

    Institute of Scientific and Technical Information of China (English)

    徐文君

    2009-01-01

    重点阐述了一种新型水膨胀防水密封胶的合成,包括丙烯酸类专用吸水树脂的组成配方和合成过程,以及以氯丁胶为主的基体胶料的选择;扼要介绍了该水膨胀防水密封胶的工程应用.%The article focuses on synthesis of a new water ex-pandable waterproofing sealant, including formulation of acrylic specific hydroscopic resin and its synthesis process and selection of neoprene -based rubbers. It also briefs the application of the sealant.

  10. Clinical and Radiological Profile of Acute Fibrinous and Organizing Pneumonia: A Retrospective Study

    Institute of Scientific and Technical Information of China (English)

    Jing-Hong Dai; Hui Li; Wei Shen; Li-Yun Miao; Yong-Long Xiao; Mei Huang; Meng-Shu Cao

    2015-01-01

    Background:Acute fibrinous and organizing pneumonia (AFOP) is a unique pathological entity with intra-alveolar fibrin in the form of "fibrin balls" and organizing pneumonia.It was divided into rare idiopathic interstitial pneumonia according to the classification notified by American Thoracic Society/European Respiratory Society in 2013.As a rare pathological entity,it is still not well known and recognized by clinicians.We reviewed the clinical features of 20 patients with AFOP diagnosed in a teaching hospital.Methods:The medical records of 20 patients with biopsy-proven diagnosis of AFOP were retrospectively reviewed.The patients' symptoms,duration of the disease,comorbidities,clinical laboratory data,pulmonary function testing,radiographic studies,and the response to treatment were extracted and analyzed.Results:Fever was the most common symptom and was manifested in 90% of AFOP patients.For clinical laboratory findings,systematic inflammatory indicators,including C-reactive protein and erythrocyte sedimentation rate,were significantly higher than normal in AFOP patients.In accordance with this increased indicators,injured liver functions were common in AFOP patients.Inversely,AFOP patients had worse clinical conditions including anemia and hypoalbuminemia.For pulmonary function testing,AFOP patients showed the pattern of restrictive mixed with obstructive ventilation dysfunction.For high-resolution computerized tomography (HRCT) findings,the most common pattern for AFOP patients was lobar consolidation which was very similar to pneumonia.However,unlike pneumonia,AFOP patients responded well to glucocorticoids.Conclusion:Patients with AFOP manifest as acute inflammatory-like clinical laboratory parameters and lobar consolidation on HRCT,but respond well to steroid.

  11. Local Application of BMP-2 Specific Plasmids in Fibrin Glue does not Promote Implant Fixation

    Directory of Open Access Journals (Sweden)

    Plank Christan

    2011-07-01

    Full Text Available Abstract Background BMP-2 is known to accelerate fracture healing and might also enhance osseointegration and implant fixation. Application of recombinant BMP-2 has a time-limited effect. Therefore, a gene transfer approach with a steady production of BMP-2 appears to be attractive. The aim of this study was to examine the effect of locally applied BMP-2 plasmids on the bone-implant integration in a non-weight bearing rabbit tibia model using a comparatively new non-viral copolymer-protected gene vector (COPROG. Methods Sixty rabbits were divided into 4 groups. All of them received nailing of both tibiae. The verum group had the nails inserted with the COPROG vector and BMP-2 plasmids using fibrin glue as a carrier. Controls were a group with fibrin glue only and a blank group. After 28 and 56 days, these three groups were sacrificed and one tibia was randomly chosen for biomechanical testing, while the other tibia underwent histomorphometrical examination. In a fourth group, a reporter-gene was incorporated in the fibrin glue instead of the BMP-2 formula to prove that transfection was successful. Results Implant fixation strength was significantly lower after 28 and 56 days in the verum group. Histomorphometry supported the findings after 28 days, showing less bone-implant contact. In the fourth group, successful transfection could be confirmed by detection of the reporter-gene in 20 of 22 tibiae. But, also systemic reporter-gene expression was found in heterotopic locations, showing an undesired spreading of the locally applied gene formula. Conclusion Our results underline the transfecting capability of this vector and support the idea that BMP-2 might diminish osseointegration. Further studies are necessary to specify the exact mechanisms and the systemic effects.

  12. The basis for fibrinogen Cedar Rapids ({gamma}R275C) fibrin network structure

    Energy Technology Data Exchange (ETDEWEB)

    DiOrio, J.P. [Baxter Healthcare Corp., Round Lake, IL (United States); Mosesson, M.W.; Siebenlist, K.R. [Univ. Wisconsin Medical School-Milwaukee Clinic Campus, WI (United States)] [and others

    1996-12-31

    Fibrinogen `Cedar Rapids` is a heterozygous dysfibrinogenemia characterized by delayed and abnormal fibrin polymerization. The specific molecular defect ({gamma}R275C) is relatively common, but in only one case, fibrinogen Tokyo II, has the ultrastructural basis for defective clot formation been determined. This report reflects similar structural studies on Cedar Rapids fibrinogen and fibrin. Crosslinked fibrinogen molecules and fibrils, were prepared at 1 mg/ml in the presence of factor XIIIa (100 u/ml). When {gamma} chains had become {approximately}10 to 20% crosslinked to {gamma} dimers, samples were diluted with Hepes buffered saline, pH 7, to a fibrinogen concentrated of 5 to 10 {mu}g/ml. Three {mu}l was then injected into 3 {mu}l buffer on a carbon-coated EM grid, the specimen allowed to attach for one minute, fluid-exchanged several times with 150 mM NH{sub 4} acetate solution, frozen in liquid nitrogen, freeze-dried, and imaged at the Brookhaven STEM facility using a 40 kv probe focused at 0.25 nm. Fibrin for scanning EM (SEM) was formed directly on carbon-formvar coated gold grids. Clots that had formed overnight were fixed with 2.5% glutaraldehyde in 0.1 M Hepes, pH 7 buffer containing 0.2% tannic acid, washed with buffer, dehydrated, CO{sub 2} critical point dried, coated with 7.5 nm platinum, and imaged in a JOEL Field Emission SEM operated at 5 kV.

  13. Altered fibrin clot structure/function in patients with antiphospholipid syndrome: association with thrombotic manifestation.

    Science.gov (United States)

    Celińska-Lowenhoff, M; Iwaniec, T; Padjas, A; Musiał, J; Undas, A

    2014-08-01

    We tested the hypothesis that plasma fibrin clot structure/function is unfavourably altered in patients with antiphospholipid syndrome (APS). Ex vivo plasma clot permeability, turbidity and susceptibility to lysis were determined in 126 consecutive patients with APS enrolled five months or more since thrombotic event vs 105 controls. Patients with both primary and secondary APS were characterised by 11% lower clot permeability (p<0.001), 4.8% shorter lag phase (p<0.001), 10% longer clot lysis time (p<0.001), and 4.7% higher maximum level of D-dimer released from clots (p=0.02) as compared to the controls. Scanning electron microscopy images confirmed denser fibrin networks composed of thinner fibres in APS. Clots from patients with "triple-antibody positivity" were formed after shorter lag phase (p=0.019) and were lysed at a slower rate (p=0.004) than in the remainder. Clots from APS patients who experienced stroke and/or myocardial infarction were 8% less permeable (p=0.01) and susceptible to lysis (10.4% longer clot lysis time [p=0.006] and 4.5% slower release of D-dimer from clots [p=0.01]) compared with those following venous thromboembolism alone. Multivariate analysis adjusted for potential confounders showed that in APS patients, lupus anticoagulant and "triple-positivity" were the independent predictors of clot permeability, while "triple-positivity" predicted lysis time. We conclude that APS is associated with prothrombotic plasma fibrin clot phenotype, with more pronounced abnormalities in arterial thrombosis. Molecular background for this novel prothrombotic mechanism in APS remains to be established.

  14. Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Puente, Pilar de la, E-mail: pilardelapuentegarcia@gmail.com [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain); Ludeña, Dolores [Pathology Service, University Hospital of Salamanca, P/San Vicente 58-182, 37007 Salamanca (Spain); López, Marta; Ramos, Jennifer; Iglesias, Javier [Tissue Bank, San Francisco Clinic Foundation, Av./Facultad 51, 5°, 24004 León (Spain)

    2013-02-01

    Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. -- Highlights: ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.

  15. Isolation from Gloydius blomhoffii siniticus Venom of a Fibrin(ogenolytic Enzyme Consisting of Two Heterogenous Polypeptides

    Directory of Open Access Journals (Sweden)

    Choi Suk-Ho

    2013-06-01

    Full Text Available Objective: This study was undertaken to isolate a fibrin(ogenolytic enzyme from the snake venom of Gloydius blomhoffii siniticus and to investigate the enzymatic characteristics and hemorrhagic activity of the isolated enzyme as a potential pharmacopuncture agent. Methods: The fibrinolytic enzyme was isolated by using chromatography, sodium dodecyl sulfatepolyacrylamide gel electrophoresis, and fibrin plate assay. The characteristics of the enzyme were determined by using fibrin plate assay, protein hydrolysis analysis, and hemorrhage assay. Its amino acid composition was determined. Results: The fibrin(ogenolytic enzyme with the molecular weight of 27 kDa (FE-27kDa isolated from G. b. siniticus venom consisted of two heterogenous disulfide bond-linked polypeptides with the molecular weights of 15 kDa and 18 kDa. When more than 20 μg of FE-27kDa was applied on the fibrin plate, fibrinolysis zone was formed as indicating its fibrinolytic activity. The fibrinolytic activity was inhibited completely by phenylmethanesulfonylfluoride (PMSF and ethylenediaminetetraacetic acid (EDTA and partially by thiothreitol and cysteine. Metal ions such as Hg2+ and Fe2+ inhibited the fibrinolytic activity completely, but Mn2+ did not. FE-27kDa preferentially hydrolyzed α-chain of fibrinogen and slowly hydrolyzed β-chain, but did not hydrolyze γ-chain. High-molecular-weight polypeptides of gelatin were hydrolyzed partially into polypeptides with molecular weights of more than 45 kDa. A dosage of more than 10 μg of FE- 27kDa per mouse was required to induce hemorrhage beneath the skin. Conclusion: FE-27kDa was a serine proteinase consisting of two heterogeneous polypeptides, hydrolyzed fibrin, fibrinogen, and gelatin, and caused hemorrhage beneath the skin of mouse. This study suggests that the potential of FE-27kDa as pharmacopuncture agent should be limited due to low fibrinolytic activity and a possible side effect of hemorrhage.

  16. Availability of the B beta(15-21) epitope on cross-linked human fibrin and its plasmic degradation products

    Science.gov (United States)

    Chen, F.; Haber, E.; Matsueda, G. R.

    1992-01-01

    The binding of radiolabeled monoclonal antifibrin antibody 59D8 (specific for fibrin but not fibrinogen) to a series of degraded fibrin clots showed that the availability of the B beta(15-21) epitope (against which 59D8 had been raised) was inversely proportional to the extent of clot lysis. Examination of digest supernatants revealed that the B beta(15-21) epitope was released from clots as a high molecular weight degradation product in the presence of calcium ions but that the generation of low molecular weight peptides occurred in the absence of calcium ions. To address the question of epitope accessibility, we compared levels of fibrin clot binding among four radioactively labeled antibodies: antifibrin monoclonal antibody 59D8, two antifibrinogen monoclonal antibodies that cross-reacted with fibrin, and an affinity-purified polyclonal antifibrinogen antibody. We expected that the antifibrinogen antibodies would show enhanced binding to clots in comparison with the antifibrin antibody. However, the epitope accessibility experiments showed that all four antibody preparations bound fibrin clots at comparable levels. Taken together, these studies demonstrated that one fibrin-specific epitope, B beta(15-21), remains available on clots as they undergo degradation by plasmin and, importantly, that the epitope is not solubilized at a rate faster than the rate at which the clot is itself solubilized. The availability of the B beta(15-21) epitope during the course of plasminolysis assures the potential utility of antifibrin antibodies such as 59D8 for detecting thrombi and targeting plasminogen activators.

  17. Fibrinogen Aα Thr312Ala polymorphism specifically contributes to chronic thromboembolic pulmonary hypertension by increasing fibrin resistance.

    Directory of Open Access Journals (Sweden)

    Ji-Feng Li

    Full Text Available BACKGROUND: Polymorphisms are associated with chronic thromboembolic pulmonary hypertension (CTEPH and pulmonary thromboembolism (PTE, but no polymorphism specific to CTEPH but not PTE has yet been reported. Fibrin resistance is associated with CTEPH, but the mechanism has not been elucidated. METHODS: Polymorphisms were analyzed in 101 CTEPH subjects, 102 PTE subjects and 108 healthy controls by Massarray or restriction fragment length polymorphism (RFLP. Plasmin-mediated cleavage of fibrin was characterized in 69 subjects (29 with CTEPH, 21 with PTE and 19 controls. RESULTS: Genotype frequencies and allele frequencies of fibrinogen Aα Thr312Ala were significantly higher in CTEPH subjects than in controls and PTE subjects, while there was no difference between PTE subjects and controls. The odd ratio (OR 2.037 and 95% confidence interval (95% CI, 1.262-3.289 showed that Thr312Ala polymorphism was a risk factor for CTEPH but not PTE. Fibrin from CTEPH subjects was more resistant to lysis than that from PTE subjects and controls. Fibrin resistance was significantly different between Aα Thr312Ala (A/G genotypes within CTEPH subjects, and the fibrin with GG genotype was more resistant than that with AA and AG genotype. CONCLUSIONS: Fibrinogen Aα Thr312Ala (A/G polymorphism was associated with CTEPH, but not PTE, suggesting that the fibrinogen Aα Thr312Ala polymorphism may act as a potential biomarker in identifying CTEPH from PTE. GG genotype polymorphism contributes to CTEPH through increasing fibrin resistance, implying that PTE subjects with fibrinogen Aα GG genotype may need long-term anticoagulation therapy.

  18. Expression of elastase and fibrin in venous leg ulcer biopsies: a pilot study of pentoxifylline versus placebo.

    Science.gov (United States)

    Mirshahi, S; Soria, J; Mirshahi, M; Soria, C; Lenoble, M; Vasmant, D; Cambazard, F; Claudy, A

    1995-01-01

    The pathogenesis of venous leg ulcers is based on the leakage of fibrinogen leading to a pericapillary fibrin cuff and plugging of capillaries by white blood cells. On the basis of a previous work, we had assumed that the key event in the pathogenesis of venous leg ulcers is related to inflammation generated by activated white blood cells that accumulate under unrelieved blood pressure, because in ulcer biopsies we had detected the presence of tumor necrosis factor-alpha (TNF-alpha) in intracapillary monocytes, elastase in the polymorphonuclear leukocytes near the vessels, and a pericapillary undegraded fibrin cuff causing a diffusion barrier to oxygen. This concept was developed because TNF-alpha synthesized by activated monocytes is responsible for many deleterious effects. It has a potent mitogenic effect on fibroblasts, leading to new collagen deposition and angiogenesis, it induces an increase in collagenase production, it acts through upregulation of an intracellular adhesion molecule (ICAM-1), leading to leukocyte sequestration and consequently a release of toxic metabolites by the polymorphonuclear cells, an early step in chronic inflammation, it activates the coagulation pathway via a marked increase in monocyte-associated tissue factor (TF) procoagulant activity, and it inhibits fibrinolysis by promoting the release of PAI-1, contributing to undegraded fibrin deposition. Therefore, we were interested in evaluating, in patients with venous leg ulcers, the effect of pentoxifylline administered at 1,200 mg daily (versus placebo) for 2-months, as this drug induces a decrease in TNF-alpha synthesis and also blocks its activity. This pilot assay was performed in blind. Evolution of several parameters in ulcer biopsies are analyzed: TNF-alpha, intact fibrin, fibrin degradation products, ICAM-1, TF, and elastase. Pentoxifylline administration induced a decrease of local elastase and of fibrin deposit. These results support the hypothesis that accumulation of

  19. A novel surgical procedure for papilla reconstruction using platelet rich fibrin

    Directory of Open Access Journals (Sweden)

    Lalitha Tanjore Arunachalam

    2012-01-01

    Full Text Available Loss of interdental papilla predisposes to phonetic, functional, and esthetic problem. Surgical techniques are manifold, but are challenging and very unpredictable. The purpose of this case report is to present the use of platelet rich fibrin (PRF in the reconstruction of papilla in the maxiallry anterior region of a forty year old woman who presented with loss of interdental papillae in 11, 12 and 11, 21 region. PRF was tucked in to the pouch created with a semilunar pedicle flap and the entire gingivopapillary unit was displaced coronally. Optimal fill was noted at 3 and 6 months postoperatively. Use of PRF may thus be the panacea for interdental papilla augmentation.

  20. Use of platelet rich fibrin in a fenestration defect around an implant

    Directory of Open Access Journals (Sweden)

    R Vijayalakshmi

    2012-01-01

    Full Text Available Guided bone regeneration (GBR in implant therapy is especially useful for implant placement with dehiscence defects or fenestration defects. In alveolar ridges with marked facial/buccal depressions or in knifeedge alveolar crests, the position and direction of fixture placement is restricted. Improvement of alveolar ridge morphology becomes possible with GBR. This article describes a case in which the fenestration defect around an implant was treated by the application of platelet rich fibrin, a second generation platelet concentrate along with bone graft, and guided tissue regeneration membrane.

  1. Postextraction socket grafting using calcium phosphate cement and platelet rich fibrin

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    Nihal Devkar

    2014-01-01

    Full Text Available This clinical case report describes and demonstrates the successful use of calcium phosphate cement (CPC in conjunction with platelet-rich fibrin (PRF for postextraction socket grafting in maxillary right first premolar area. CPC can be molded to form a scaffold. It has been used previously for regeneration in intrabony defects, but very few clinical studies in humans have reported its use for socket grafting. In this report, we have presented a novel use of CPC in conjunction with PRF for ridge preservation after tooth extraction.

  2. Successful application of subcutaneous adipose tissue with fibrin glue in conservative treatment of tracheobronchial rupture.

    Science.gov (United States)

    Tokuishi, Keita; Yamamoto, Satoshi; Anami, Kentaro; Moroga, Toshihiko; Miyawaki, Michiyo; Chujo, Masao; Yamashita, Shin-Ichi; Kawahara, Katsunobu

    2012-11-01

    An 84-year-old woman underwent aortic and mitral valve replacement. After weaning from cardiopulmonary bypass, hemorrhage was observed in the endobronchial tube. The bleeding bronchus was isolated to protect the airway using the blocker cuff of a Univent tube (Fuji Systems Corp, Tokyo, Japan). Computed tomography showed a pulmonary pseudoaneurysm in the left upper lobe. She underwent selective pulmonary angiography and embolization of the pseudoaneurysm. Bronchoscopy revealed a 5-mm bronchial rupture at the left upper lobe bronchus. The laceration was filled with adipose tissue and fibrin glue. Bronchoscopy showed a completely reepithelialized membrane, and she was discharged 38 days postoperatively.

  3. Platelet-rich fibrin: Evolution of a second-generation platelet concentrate

    Directory of Open Access Journals (Sweden)

    Sunitha Raja V

    2008-01-01

    Full Text Available Platelet-rich plasma (PRP is a platelet concentrate that has been used widely to accelerate soft-tissue and hard-tissue healing. The preparation of PRP has been described by several authors. Platelet-rich fibrin (PRF was first described by Choukroun et al. in France. It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared PRP. Its chief advantages include ease of preparation and lack of biochemical handling of blood, which makes this preparation strictly autologous. This article describes the evolution of this novel platelet concentrate, referred to as PRF.

  4. Soluble fibrin inhibits monocyte adherence and cytotoxicity against tumor cells: implications for cancer metastasis

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    Patel Shonak

    2006-08-01

    Full Text Available Abstract Background Soluble fibrin (sFn is a marker for disseminated intravascular coagulation and may have prognostic significance, especially in metastasis. However, a role for sFn in the etiology of metastatic cancer growth has not been extensively studied. We have reported that sFn cross-linked platelet binding to tumor cells via the major platelet fibrin receptor αIIbβ3, and tumor cell CD54 (ICAM-1, which is the receptor for two of the leukocyte β2 integrins (αLβ2 and aMβ2. We hypothesized that sFn may also affect leukocyte adherence, recognition, and killing of tumor cells. Furthermore, in a rat experimental metastasis model sFn pre-treatment of tumor cells enhanced metastasis by over 60% compared to untreated cells. Other studies have shown that fibrin(ogen binds to the monocyte integrin αMβ2. This study therefore sought to investigate the effect of sFn on β2 integrin mediated monocyte adherence and killing of tumor cells. Methods The role of sFn in monocyte adherence and cytotoxicity against tumor cells was initially studied using static microplate adherence and cytotoxicity assays, and under physiologically relevant flow conditions in a microscope perfusion incubator system. Blocking studies were performed using monoclonal antibodies specific for β2 integrins and CD54, and specific peptides which inhibit sFn binding to these receptors. Results Enhancement of monocyte/tumor cell adherence was observed when only one cell type was bound to sFn, but profound inhibition was observed when sFn was bound to both monocytes and tumor cells. This effect was also reflected in the pattern of monocyte cytotoxicity. Studies using monoclonal blocking antibodies and specific blocking peptides (which did not affect normal coagulation showed that the predominant mechanism of fibrin inhibition is via its binding to αMβ2 on monocytes, and to CD54 on both leukocytes and tumor cells. Conclusion sFn inhibits monocyte adherence and cytotoxicity of

  5. KEBERHASILAN PENETRASI BAHAN PIT & FISSURE SEALANT KE DALAM FISURA MENGGUNAKAN SYRINGE BLUE MICRO TIPS DAN SYRINGE WHITE MINI BRUSH TIPS (LAPORAN PENELITIAN)

    OpenAIRE

    2015-01-01

    The aim of this study was to know the success and differences of pit & fissure sealant materal penetration of composite resin type (UltraSeal XT Plus) into fissure by using syringe blue micro tips and syringe white mini brush tips in fissure closure efforts for caries prevention of permanent teeth. The study was conducted to 30 maxillary first premolar, using syring blue micro tips, and 30 maxilary first premolar using syringw white mini brush tips. The samples, then, were made smears and...

  6. Comparative evaluation of Shear bond strength of different Pit and fissure Sealants in Primary and Permanent teeth - An In-Vitro Study

    OpenAIRE

    Pushpalatha, H M; Ravichandra, K S; Srikanth, Koya; G. Divya; Done, Vasanthi; Krishna, K Bala; Patil, Vishwanath

    2014-01-01

    Background: Dental caries among children is one of the greatest challenges faced by dentists globally; especially that of susceptible surfaces like the Pit and fissures. Dental sealants have proved to be an effective way to prevent caries development. The Clinical success of any material depends upon its adhesion to tooth structure, resistance to wear and ability to withstand the masticatory or occlusal forces. Hence it is important to evaluate the shear bond strength ...

  7. Fibrin and poly(lactic-co-glycolic acid hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study

    Directory of Open Access Journals (Sweden)

    Idrus Ruszymah BH

    2008-04-01

    Full Text Available Abstract Background Synthetic- and naturally derived- biodegradable polymers have been widely used to construct scaffolds for cartilage tissue engineering. Poly(lactic-co-glycolic acid (PLGA are bioresorbable and biocompatible, rendering them as a promising tool for clinical application. To minimize cells lost during the seeding procedure, we used the natural polymer fibrin to immobilize cells and to provide homogenous cells distribution in PLGA scaffolds. We evaluated in vitro chondrogenesis of rabbit articular chondrocytes in PLGA scaffolds using fibrin as cell transplantation matrix. Methods PLGA scaffolds were soaked in chondrocytes-fibrin suspension (1 × 106cells/scaffold and polymerized by dropping thrombin-calcium chloride (CaCl2 solution. PLGA-seeded chondrocytes was used as control. All constructs were cultured for a maximum of 21 days. Cell proliferation activity was measured at 1, 3, 7, 14 and 21 days in vitro using 3-(4,5-dimethylthiazole-2-yl-2-, 5-diphenyltetrazolium-bromide (MTT assay. Morphological observation, histology, immunohistochemistry (IHC, gene expression and sulphated-glycosaminoglycan (sGAG analyses were performed at each time point of 1, 2 and 3 weeks to elucidate in vitro cartilage development and deposition of cartilage-specific extracellular matrix (ECM. Results Cell proliferation activity was gradually increased from day-1 until day-14 and declined by day-21. A significant cartilaginous tissue formation was detected as early as 2-week in fibrin/PLGA hybrid construct as confirmed by the presence of cartilage-isolated cells and lacunae embedded within basophilic ECM. Cartilage formation was remarkably evidenced after 3 weeks. Presence of cartilage-specific proteoglycan and glycosaminoglycan (GAG in fibrin/PLGA hybrid constructs were confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrix. Chondrogenic properties were further

  8. Pluripotential differentiation capability of human adipose-derived stem cells in a novel fibrin-agarose scaffold.

    Science.gov (United States)

    Nieto-Aguilar, R; Serrato, D; Garzón, I; Campos, A; Alaminos, M

    2011-03-01

    The potentiality of adipose-derived stem cells (ASCs) cultured on 2D systems has been previously established. Nevertheless, very little is known so far about the differentiation potentiality of ASCs in 3D culture systems using biomaterials. In this work, we have evaluated the transdifferentiation capabilities of ASCs cultured within a novel fibrin-agarose biomaterial by histological analysis, histochemistry and immunofluorescence. Our results showed that 3D fibrin-agarose biomaterial is highly biocompatible and supports the transdifferentiation capabilities of ASCs to the osteogenic, chondrogenic, adipogenic, and neurogenic lineages.

  9. Application of glass ionomer and light-cured resin sealant to the pit and fissure of deciduous teeth%玻璃离子与光固化树脂封闭剂封闭乳牙窝沟

    Institute of Scientific and Technical Information of China (English)

    任飞; 刘建平; 黄少宏; 李艳容; 范卫华; 陈晓春; 陈清

    2011-01-01

    背景:传统方法玻璃离子作为封闭剂耐磨性能差,抗折强度低,位于合面的封闭剂很容易脱落,很难取代树脂封闭剂.目的:观察光固化树脂封闭剂和非创伤性充填技术玻璃离子窝沟封闭幼儿乳牙的效果.方法:按同人半口随机比较的方法,对89名3岁幼儿的左或右半口符合窝沟封闭条件的乳磨牙在非创伤性充填技术下行玻璃离子窝沟封闭,对侧半口符合窝沟封闭条件的乳磨牙进行树脂封闭.结果与结论:窝沟封闭后6,18个月玻璃离子组封闭物脱落率均低于树脂材料组(P < 0.05).玻璃离子材料最易脱落的牙位是下颌第二乳磨牙;树脂材料最易脱落的牙位是上颌第二乳磨牙.封闭后6个月,玻璃离子组封闭的乳牙患龋率低于树脂材料组,18个月时两组间差异无显著性意义.说明非创伤性充填技术玻璃离子窝沟封闭幼儿乳牙的脱落率低,操作简单,防龋效果肯定,且成本低,在经济学上可行性优于树脂封闭.%BACKGROUND: Traditional glass ion sealant has a poor abradability and a low rupture strength. The sealant on the occlusal surfaces easily fell off, and is difficult to replace resin sealant. OBJECTIVE: To observe the effects of traditional resin sealant and atraumatic restorative treatment (ART) glass ionomer-based pit and fissure sealant for the young children. METHODS: Randomized comparison method was used to compare ART glass ionomer-based pit on molars of one side with resin sealant on the opposite side in 89 3-year-old children. RESULTS AND CONCLUSION: The retention rates of ART glass ionomer sealant after 6 and 18 months were significantly lower than those of resin sealant (P < 0.05). The caducous position of ART gliass ionomer sealant was the second deciduous molar of the lower mandible, but the caducous position of resin sealant was the second deciduous molar of the upper mandible. The secondary caries rate of ART glass ionomer sealant was significantly

  10. The impact of Er:YAG laser enamel conditioning on the microleakage of a new hydrophilic sealant--UltraSeal XT hydro.

    Science.gov (United States)

    Güçlü, Z A; Dönmez, N; Tüzüner, T; Odabaş, M E; Hurt, A P; Coleman, N J

    2016-05-01

    UltraSeal XT hydro is a new hydrophilic, light-cured, methacrylate-based pit and fissure sealant which has been developed by Ultradent Products, USA. The sealant is highly filled with a 53 wt.% mixture of inorganic particles which confer both thixotropy and radiopacity. The principal purpose of this study was to investigate the microleakage of UltraSeal XT hydro as a function of different enamel etching techniques. The occlusal surfaces of sound, extracted human molars were either acid etched, Er:YAG laser irradiated or successively laser irradiated and acid etched. UltraSeal XT hydro was applied to each group of teeth (n = 10) which were subjected to a thermocycling process consisting of 2500 cycles between 5 and 50 °C with a dwell time of 30 s. Microleakage assessments were then carried out using 0.5% fuchsin dye and optical microscopy. The microleakage score data were analysed using the Kruskal-Wallis, Mann-Whitney U test with Bonferroni adjustment. No significant differences in microleakage were noted between the individually acid etched and laser-irradiated groups (p > 0.05); however, teeth treated with a combination of laser irradiation and acid etching demonstrated significantly lower microleakage scores (p Laser etching increases the roughness of the enamel surface which causes a concentrated zoning of the filler particles at the enamel-sealant interface.

  11. Effect of the application of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste and adhesive systems on bond durability of a fissure sealant.

    Science.gov (United States)

    Borges, Boniek Castillo Dutra; Catelan, Anderson; Sasaki, Robson Tetsuo; Ambrosano, Gláucia Maria Bovi; Reis, André Figueiredo; Aguiar, Flávio Henrique Baggio

    2013-01-01

    This study aimed to evaluate the effect of the previous application of a casein phosphopeptide-amorphous calcium phosphate paste (MI Paste, MI) and adhesive systems on the bond durability of a fissure sealant. Ninety-eight enamel blocks were obtained from proximal surfaces of erupted third molars. Specimens were divided into 14 groups (n = 7) according to the previous application of MI (with and without) and the adhesive systems used (no adhesive system; hydrophobic resin of a three-step etch-and-rinse adhesive system; etch-and-rinse single-bottle adhesive system; all-in-one adhesive system; two-step self-etching adhesive system; additional phosphoric acid conditioning and all-in-one adhesive system; additional phosphoric acid conditioning and two-step self-etching adhesive system). A fissure sealant (Fluroshield) was applied and photoactivated for 20 s. Beams (~0.7 mm(2)) were prepared for the microtensile bond strength test, which was executed after 24 h or 6 months of water storage. Fractured specimens were analyzed by scanning electronic microscopy. Data were analyzed by two-way ANOVA with repeated measures/Tukey's test (P adhesive systems presented higher means than those groups where MI was not applied. Higher frequency of cohesive failures was observed for groups with MI. Applying a CPP-ACP containing paste on enamel before adhesive systems was an effective method to increase bond durability of the sealant tested.

  12. Preparation of One-component Polyurethane Foam Sealant%单组分聚氨酯泡沫填缝剂的制备

    Institute of Scientific and Technical Information of China (English)

    王德鹏; 杨猛; 冯艳

    2013-01-01

    In order to develop high-performance one-component polyurethane foam sealant, influences of composition of mixed polyether, amount of flame retardant, amount of catalyst, and the molar ratio of isocyanate to hydroxyl on properties of one-component polyurethane foam sealant were studied. And the optimum combination of raw materials was obtained. The results show that the excellent one-component polyurethane foam sealant with fast curing, high strength, good dimensional stability and low temperature foaming performance can be prepared under the optimum conditions.%  为研制出综合性能优异的单组分聚氨酯泡沫填缝剂,对混合聚醚的组成、阻燃剂加入量、催化剂加入量及异氰酸根与羟基的摩尔比对单组分聚氨酯填缝剂性能的影响进行了系统的研究,最终优选出了最佳组合,研制出了快固化、高强度、尺寸稳定性好及优良的低温发泡性能的综合性能优异的单组分聚氨酯泡沫填缝剂。

  13. Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics

    Directory of Open Access Journals (Sweden)

    Dexton Antony Johns

    2014-01-01

    Full Text Available Aim: Photoactivated disinfection has been used as an adjunct to conventional endodontic treatment. Its use in regenerative endodontics is not reported in literature. The aim of this case report was to describe a new proposal for pulp revascularization with disinfection of pulp canal space using a unique combination of a photosensitizer solution and low-power laser light. Materials and Methods: A 9-year-old boy came with the chief complaint of discolored upper central incisors (#8, #9. A diagnosis of pulp necrosis was made on the basis of clinical and radiographic findings. The canal was irrigated with 5.25% sodium hypochlorite solution and dried with paper points. Photodynamic therapy was used to disinfect the root canal and platelet-rich fibrin was used to revitalize the pulp. Three millimeters of gray mineral trioxide aggregate was placed directly over the platelet-rich plasma clot. Three days later, the tooth was double-sealed with permanent filling materials. Results: Clinical examination revealed no sensitivity to percussion or palpation tests. Radiograph revealed continued thickening of the dentinal walls, root lengthening, regression of the peri-apical lesion and apical closure. Both the roots showed complete apical closure at the 10-month follow-up. However, the teeth were not responsive to electric pulp test. Conclusion: This report of pulp revascularization shows that disinfection with photodynamic therapy combined with platelet-rich fibrin leads to satisfactory root development in necrotic immature teeth.

  14. Effect of intraoperative platelet-rich plasma and fibrin glue application on skin flap survival.

    Science.gov (United States)

    Findikcioglu, Fulya; Findikcioglu, Kemal; Yavuzer, Reha; Lortlar, Nese; Atabay, Kenan

    2012-09-01

    The experiment was designed to compare the effect of intraoperative platelet-rich plasma (PRP) and fibrin glue application on skin flap survival. In this study, bilateral epigastric flaps were elevated in 24 rats. The right-side flaps were used as the control of the left-side flaps. Platelet-rich plasma, fibrin glue, and thrombin had been applied under the flap sites in groups 1, 2, and 3, respectively. Five days later, all flap pedicles were ligated. Necrotic area measurements, microangiography, and histologic and immunohistochemical evaluations were performed to compare the groups. Platelet-rich plasma reduced necrotic area percentages as compared with other groups. Histologically and microangiographically increased number of arterioles were observed in PRP groups. Thrombin when used alone increased flap necrosis. Vascular endothelial growth factor, platelet-derived growth factor, and transforming growth factor β3 primary antibody staining showed increased neovascularization and reepithelialization in all PRP-applied flaps. This study demonstrated that PRP, when applied intraoperatively under the skin flap, may enhance flap survival. Thrombin used alone was found to be unsuitable in flap surgery.

  15. EFFICACY OF THROMBIN FIBRIN GLUE AND SCLE ROSANT IN THE MANAGEMENT OF BLEEDI NG GASTRIC VARICES

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    Sanjay Gupta

    2015-01-01

    Full Text Available Gastric varices are noted in up to 20 % of patents with portal hypertension , and are more common in those with non - cirrhotic etiology 1 . They bleed at lower portal pressures , bleed more severely and are associated with higher rates of rebleed , encephalopathy and mortality 1,2,3 . Variceal obliteration using tissue adhesives such as N - butyl cyanoacrylate leading to plugging and thrombosis of the gastric varices is currently the first line management option for obliteration of the gastric varices 3 . Although various options have been proposed , gold standard for management of gastric variceal bleeds is yet to be defined. We theorized that injection of the gastric varices using thrombin based glue followed by injection of a sclerosant shall be effective in optimum sclerotherapy and eradication of gastric varices. MATERIAL AND METHODS : All patients presenting with gastric variceal bleed were offered sclerotherapy with Thrombin fibrin based glue and sclerosant (TFG/S . During the study period 18 patients were enrolled in the TGF/S group. 21 patients underwent variceal plugging with n - butyl cyanoacrylate (NBC . There was no significant difference in age/ sex , duration of bleed or time interval between onset of bleed and endotherapy. RESULTS: Patients undergoing endotherapy with TGF/S had less episodes of bleed , and greater eradication of varices. CONCLUSION: The results with thrombin / fibrin glue and sclerotherapy are highly encouraging. Well - designed trials need to be performed KEYWORDS:Gastric varices; Thrombin Sclerotherapy

  16. Selective inhibition by a synthetic hirudin peptide of fibrin-dependent thrombosis in baboons

    Energy Technology Data Exchange (ETDEWEB)

    Cadroy, Y.; Hanson, S.R.; Harker, L.A. (Emory Univ., Atlanta, GA (United States)); Maraganore, J.M. (Biogen Inc., Cambridge, MA (United States))

    1991-02-15

    To determine the importance of the thrombin substrate recognition exosite for fibrinogen binding in the formation of both arterial and venous thrombi the authors evaluated the antithrombotic effects of the tyrosine-sulfated dodecapeptide from residues 53-64 of hirudin (H peptide) in a nonhuman primate model. This peptide was studied because it inhibits thrombin cleavages of fibrinogen by simple competition without blocking enzyme catalytic-site function. When an exteriorized arteriovenous access shunt model was used in baboons (Papio anubis), thrombus formation was induced by placing a thrombogenic device made of (i) a segment of tubing coated covalently with type I collagen, which generated platelet-rich thrombi under arterial flow conditions, and (ii) two subsequent annular regions of flow expansion that produced fibrin-rich thrombi typically associated with venous valves and veins. Thrombus formation was quantified by measurements of {sup 111}In-labeled platelet and {sup 125}I-labeled fibrinogen deposition in both arterial-flow and venous-flow portions of the device. These finding suggest that, by competitive inhibition of fibrinogen binding to thrombin, fibrin-rich venous-type thrombus formation may be selectively prevented. This strategy may be therapeutically attractive for preserving normal platelet function when conventional anticoagulant therapy is contraindicated.

  17. Treatment of Peri-implant Bone Defects with Platelet-Rich Fibrin.

    Science.gov (United States)

    Hamzacebi, Burak; Oduncuoglu, Bahar; Alaaddinoglu, Emine Elif

    2015-01-01

    The objective of this study was to investigate and compare the clinical effectiveness of the application of platelet-rich fibrin (PRF) and conventional flap surgery for the treatment of peri-implant bone loss. Nineteen patients (8 women, 11 men) with peri-implant bone loss were randomly allocated to two groups, with the PRF group comprising patients who received fibrin scaffold and the control group made up of those who received only the access flap. At 3 and 6 months after surgery, respectively, the PRF group demonstrated higher mean probing depth reductions (2.41 ± 1.06 and 2.82 ± 1.03 mm versus 1.65 ± 1.02 and 2.05 ± 0.77 mm) and more gains in clinical attachment level (2.89 ± 1.01 and 3.31 ± 1.08 mm versus 1.43 ± 1.08 and 1.84 ± 0.81 mm) compared with the control group. In addition, the increase in the amount of keratinized mucosa from baseline to 6 months postoperatively was statistically significant for the PRF group (P < .001). Hence, the data from the current study led to the conclusion that PRF application in periimplant bone loss provided better clinical results than conventional flap surgery.

  18. Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration.

    Science.gov (United States)

    Kang, Young-Ho; Jeon, Soung Hoo; Park, Joo-Young; Chung, Jong-Hoon; Choung, Yun-Hoon; Choung, Han-Wool; Kim, Eun-Suk; Choung, Pill-Hoon

    2011-02-01

    The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. However, the microstructure of PRF has not been fully investigated nor have been studied the key molecules that differ PRF from platelet-rich plasma. We fabricated PRF under Choukroun's protocol and produced its extract (PRFe) by freezing at -80°C. The conventional histological, immunohistological staining, and scanning electron microscopy images showed the microstructure of PRF, appearing as two zones, the zone of platelets and the zone of fibrin, which resembled a mesh containing blood cells. The PRFe increased proliferation, migration, and promoted differentiation of the human alveolar bone marrow stem cells (hABMSCs) at 0.5% concentration in vitro. From the results of proteome array, matrix metalloproteinase 9 (MMP9) and Serpin E1 were detected especially in PRFe but not in concentrated platelet-rich plasma. Simultaneous elevation of MMP9, CD44, and transforming growth factor β-1 receptor was shown at 0.5% PRFe treatment to the hABMSC in immunoblot. Mineralization assay showed that MMP9 directly regulated mineralization differentiation of hABMSC. Transplantation of the fresh PRF into the mouse calvarias enhanced regeneration of the critical-sized defect. Our results strongly support the new characteristics of PRF as a bioscaffold and reservoir of growth factors for tissue regeneration.

  19. The use of fibrin and poly(lactic-co-glycolic acid hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis

    Directory of Open Access Journals (Sweden)

    S Munirah

    2008-02-01

    Full Text Available Our preliminary results indicated that fibrin and poly(lactic-co-glycolic acid (PLGA hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl2 solution. PLGA-seeded chondrocytes were used as a control. Resulting constructs were implanted subcutaneously, at the dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and sulphated-glycosaminoglycan (sGAG analyses were performed at each time point of 1, 2 and 4 weeks post-implantation. Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and glycosaminoglycan (GAG in fibrin/PLGA hybrid constructs was confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene encoded cartilage-specific markers, collagen type II and aggrecan core protein. The sGAG production in fibrin/PLGA hybrid constructs was higher than in the PLGA group. In conclusion, fibrin/PLGA hybrid scaffold promotes cartilaginous tissue formation in vivo and may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering.

  20. Binding of thrombin-activated platelets to a fibrin scaffold through α(IIbβ₃ evokes phosphatidylserine exposure on their cell surface.

    Directory of Open Access Journals (Sweden)

    Tomasz Brzoska

    Full Text Available Recently, by employing intra-vital confocal microscopy, we demonstrated that platelets expose phosphatidylserine (PS and fibrin accumulate only in the center of the thrombus but not in its periphery. To address the question how exposure of platelet anionic phospholipids is regulated within the thrombus, an in-vitro experiment using diluted platelet-rich plasma was employed, in which the fibrin network was formed in the presence of platelets, and PS exposure on the platelet surface was analyzed using Confocal Laser Scanning Microscopy. Almost all platelets exposed PS after treatment with tissue factor, thrombin or ionomycin. Argatroban abrogated fibrin network formation in all samples, however, platelet PS exposure was inhibited only in tissue factor- and thrombin-treated samples but not in ionomycin-treated samples. FK633, an α(IIbβ₃ antagonist, and cytochalasin B impaired platelet binding to the fibrin scaffold and significantly reduced PS exposure evoked by thrombin. Gly-Pro-Arg-Pro amide abrogated not only fibrin network formation, but also PS exposure on platelets without suppressing platelet binding to fibrin/fibrinogen. These results suggest that outside-in signals in platelets generated by their binding to the rigid fibrin network are essential for PS exposure after thrombin treatment.

  1. Assessment of the effect on blood loss and transfusion requirements when adding a polyethylene glycol sealant to the anastomotic closure of aortic procedures: a case–control analysis of 102 patients undergoing Bentall procedures

    Directory of Open Access Journals (Sweden)

    Natour Ehsan

    2012-10-01

    Full Text Available Abstract Background The use of CoSeal®, a polyethylene glycol sealant, in cardiac and vascular surgery for prevention of anastomotic bleeding has been subject to prior investigations. We analysed our perioperative data to determine the clinical benefit of using polyethylene glycol sealant to inhibit suture line bleeding in aortic surgery. Methods From January 2004 to June 2006, 124 patients underwent aortic surgical procedures such as full root replacements, reconstruction and/or replacement of ascending aorta and aortic arch procedures. A Bentall procedure was employed in 102 of these patients. In 48 of these, a polyethylene glycol sealant was added to the anastomotic closure of the aortic procedure (sealant group and the other 54 patients did not have this additive treatment to the suture line (control group. Results There were no significant between-group differences in the demographic characteristics of the patients undergoing Bentall procedures. Mean EuroSCORES (European System for Cardiac Operative Risk Evaluation were 13.7 ± 7.7 (sealant group and 14.4 ± 6.2 (control group, p = NS. The polyethylene glycol sealant group had reduced intraoperative and postoperative transfusion requirements (red blood cells: 761 ± 863 versus 1248 ± 1206 ml, p = 0.02; fresh frozen plasma: 413 ± 532 versus 779 ± 834 ml, p = 0.009; and less postoperative drainage loss (985 ± 972 versus 1709 ± 1302 ml, p = 0.002. A trend towards a lower rate of rethoracotomy was observed in the sealant group (1/48 versus 6/54, p = 0.07 and there was significantly less time spent in the intensive care unit or hospital (both p = 0.03. Based on hypothesis-generating calculations, the resulting economic benefit conferred by shorter intensive care unit and hospital stays, reduced transfusion requirements and a potentially lower rethoracotomy rate is estimated at €1,943 per patient in this data analysis

  2. Transplantation of adipose-derived stem cells with fibrin glue for treatment of acute myocardial infarction in rat

    Institute of Scientific and Technical Information of China (English)

    张雪莲

    2013-01-01

    Objective To investigate the cell survival of the combination of fibrin glue and adiposederived stem cells(ADSCs) in rats when implanted into ischemic myocardium and the improvement of heart function. Methods The rat ADSCs were isolated from the subcutaneous adipose

  3. Management of urine leak after laparoscopic cyst decortication with retrograde endoscopic fibrin glue application and ureteral stent placement.

    Science.gov (United States)

    Chen, Mang L; Tomaszewski, Jeffrey J; Matoka, Derek J; Ost, Michael C

    2011-01-01

    Urine leakage is an uncommon complication after renal cyst decortication that typically resolves with adequate drainage. With prolonged large volume urine leakage from a perinephric drain, however, consideration for open surgical repair must be taken into account. We present the successful management of persistent urine leakage after laparoscopic cyst decortication with endoscopic retrograde fibrin glue injection and ureteral stent placement.

  4. The rigid curette technique for the application of fibrin bioadhesive during hip arthroscopy for articular cartilage lesions.

    Science.gov (United States)

    Asopa, Vipin; Singh, Parminder J

    2014-04-01

    Encouraging midterm results have recently been reported for the arthroscopic treatment of delaminating articular cartilage lesions at the capsulolabral junction of the hip joint using fibrin bioadhesive. The needle used to introduce the bioadhesive is long, flexible, and often difficult to position. We describe a novel technique for introducing the needle that allows accurate placement behind the delaminated articular cartilage pocket during hip arthroscopy.

  5. Reduction of bone resorption by the application of fibrin glue in the reconstruction of the alveolar cleft.

    Science.gov (United States)

    Segura-Castillo, José L; Aguirre-Camacho, Humberto; González-Ojeda, Alejandro; Michel-Perez, Jorge

    2005-01-01

    A major complication in 30% to 75% of cases of surgical treatment of alveolar cleft is resorption of the bone graft. A treatment alternative is the application of fibrin glue, which has the capacity to favor the integration of the graft. The main objective of the study was to evaluate if the use of the fibrin glue reduces bone resorption when it is applied locally. The authors designed a randomized clinical trial. Patients were divided into two groups: group 1, fibrin glue; and group 2, control. Pre- and postoperative graft volume, bone density, bone quality (Lekholm and Zarb, and Norton and Gamble classifications), and postoperative complications were evaluated. The follow-up for all patients was 3 months after discharge. Twenty-seven patients were surgically treated, 13 in group 1 and 14 in group 2. Group 1 had increased graft volume compared with group 2 (64.32 cm v 21.70 cm; P 0.076). Bone quality was type 1, 2 and 3 and 4 in group 1. Resorption in group 2 was 62.26%; in group 1, it was 29.72% (P > 0.081). The observed complications were infection and dehiscence of sutures (P > 0.537). The authors conclude that the fibrin glue significantly diminishes bone resorption, allowing improved graft integration and quality.

  6. The fibrin-derived peptide Bbeta(15-42) significantly attenuates ischemia-reperfusion injury in a cardiac transplant model.

    NARCIS (Netherlands)

    Wiedemann, D.; Schneeberger, S.; Friedl, P.H.A.; Zacharowski, K.; Wick, N.; Boesch, F.; Margreiter, R.; Laufer, G.; Petzelbauer, P.; Semsroth, S.

    2010-01-01

    BACKGROUND: The inflammatory response after prolonged ischemia and subsequent reperfusion leads to increased risk of primary organ dysfunction after cardiac transplantation. It has been demonstrated that the fibrin-derived peptide Bbeta(15-42) (also called FX06) reduces infarct size in coronary arte

  7. High-density cholesterol and apolipoprotein AI as modifiers of plasma fibrin clot properties in apparently healthy individuals.

    Science.gov (United States)

    Ząbczyk, Michał; Hońdo, Łukasz; Krzek, Marzena; Undas, Anetta

    2013-01-01

    Low high-density lipoprotein cholesterol (HDL-C) increases cardiovascular risk, whereas its high levels protect against atherosclerosis via multiple beneficial effects. Dense and poorly lysable fibrin clot formation is observed in cardiovascular disease. We sought to investigate whether HDL-C and its major component apolipoprotein A (Apo A)-I affect fibrin clot properties. In 136 apparently healthy individuals (99 men, 37 women, aged 49-69 years) we determined plasma fibrin clot permeability (Ks coefficient) and lysis time (t50%) together with Apo A-I and lipoprotein (a) [Lp(a)] levels. The median HDL-C level was 1.33  mmol/l (range from 0.77 to 2.19  mmol/l). HDL-C was positively associated with Apo A-I (r = 0.62, P Ks (r = 0.52, P Ks and t50% were associated with Lp(a) (r = -0.42, P Ks (P = 0.00016) and 17% shorter t50% (P = 0.0012) than the remainder. After adjustment for age, fibrinogen, and Lp(a), HDL-C was the independent predictor of Ks (β = 0.7, P < 0.00001) and t50% (β = -0.62, P < 0.00001). This study shows that elevated HDL-C levels are associated with improved fibrin clot permeability and lysis, indicating a novel antithrombotic mechanism underlying the postulated beneficial effects of therapy targeted at HDL-C.

  8. Tunable Controlled Release of Bioactive SDF-1α via Protein Specific Interactions within Fibrin/Nanoparticle Composites.

    Science.gov (United States)

    Dutta, D; Fauer, C; Mulleneux, H L; Stabenfeldt, S E

    2015-10-31

    The chemokine, stromal cell-derived factor 1α (SDF-1α), is a key regulator of the endogenous neural progenitor/stem cell-mediated regenerative response after neural injury. Increased and sustained bioavailability of SDF-1α in the peri-injury region is hypothesized to modulate this endogenous repair response. Here, we describe poly(lactic-co-glycolic) acid (PLGA) nanoparticles capable of releasing bioactive SDF-1α in a sustained manner over 60days after a burst of 23%. Moreover, we report a biphasic cellular response to SDF-1α concentrations thus the large initial burst release in an in vivo setting may result in supratherapeutic concentrations of SDF-1α. Specific protein-protein interactions between SDF-1α and fibrin (as well as its monomer, fibrinogen) were exploited to control the magnitude of the burst release. Nanoparticles embedded in fibrin significantly reduced the amount of SDF-1α released after 72 hrs as a function of fibrin density. Therefore, the nanoparticle/fibrin composites represented a means to independently tune the magnitude of the burst phase release from the nanoparticles while perserving a bioactive depot of SDF-1α for release over 60days.

  9. Use of an autologous leucocyte and platelet-rich fibrin patch on hard-to-heal DFUs

    DEFF Research Database (Denmark)

    Löndahl, M; Tarnow, L.; Karlsmark, T;

    2015-01-01

    OBJECTIVE: Leucopatch is a leukocyte and platelet-rich fibrin patch that provides concentrated blood cells and signal substances to the surface of an ulcer. It is produced by centrifugation of the patient's own venous blood. The aim of this pilot multicentre cohort study was to evaluate effects...

  10. Nd:YAG Laser Lysis of the Fibrinous Membrane and Remnant Substance on the Anterior Surface of Intraocular Lens

    Institute of Scientific and Technical Information of China (English)

    ZhendeLin; ChenjinJin

    1995-01-01

    Purpose:To determine the effects of ND:YAGlaser to disrupt the fibrinous membrane and remnant substance on the anterior surface of intraocular lens.Methods:Nd:YAGlaser was applied on 23cases of fibrinous membrane forma-tion and 8cases of remnant substance on the anterior surface of intaocular lenses(IOL)which had not reponded well to steroid therapy,Eighteen cases were male and 13female.The mean age was49.7years(range,5-78years),The in-terval between IOL implantation and laser therapy was 0.5-30months in the fibrinous membrane cases and 3-10days in the remnant substance cases.The energy applied was0.8-3.0mJ/exp.with2-112exposures.mean follow-up period was3.6months.Results:Complications during therapy included only 2cases of slight iris bleed-ing,Visual acuity after therapy was improved 1line in 16cases,2lines in 11cas-es,3lines in 1case,4lines in 1case and 5lines in 2cases,No post-therapy com-plication was found.Conclusions:Nd:YAG laser lysis is an effective alternative to ramove the fibri-nous membrane and remnant substances on the anterior surface of IOL.Eye Sci-ence1995;11:128-130.

  11. Histidine-rich glycoprotein binds fibrin(ogen) with high affinity and competes with thrombin for binding to the gamma'-chain.

    Science.gov (United States)

    Vu, Trang T; Stafford, Alan R; Leslie, Beverly A; Kim, Paul Y; Fredenburgh, James C; Weitz, Jeffrey I

    2011-09-01

    Histidine-rich glycoprotein (HRG) is an abundant protein that binds fibrinogen and other plasma proteins in a Zn(2+)-dependent fashion but whose function is unclear. HRG has antimicrobial activity, and its incorporation into fibrin clots facilitates bacterial entrapment and killing and promotes inflammation. Although these findings suggest that HRG contributes to innate immunity and inflammation, little is known about the HRG-fibrin(ogen) interaction. By immunoassay, HRG-fibrinogen complexes were detected in Zn(2+)-supplemented human plasma, a finding consistent with a high affinity interaction. Surface plasmon resonance determinations support this concept and show that in the presence of Zn(2+), HRG binds the predominant γ(A)/γ(A)-fibrinogen and the γ-chain elongated isoform, γ(A)/γ'-fibrinogen, with K(d) values of 9 nm. Likewise, (125)I-labeled HRG binds γ(A)/γ(A)- or γ(A)/γ'-fibrin clots with similar K(d) values when Zn(2+) is present. There are multiple HRG binding sites on fibrin(ogen) because HRG binds immobilized fibrinogen fragment D or E and γ'-peptide, an analog of the COOH terminus of the γ'-chain that mediates the high affinity interaction of thrombin with γ(A)/γ'-fibrin. Thrombin competes with HRG for γ'-peptide binding and displaces (125)I-HRG from γ(A)/γ'-fibrin clots and vice versa. Taken together, these data suggest that (a) HRG circulates in complex with fibrinogen and that the complex persists upon fibrin formation, and (b) by competing with thrombin for γ(A)/γ'-fibrin binding, HRG may modulate coagulation. Therefore, the HRG-fibrin interaction may provide a novel link between coagulation, innate immunity, and inflammation.

  12. Development of a One-package Epoxy Resin Sealant for Electronic Memory Chips%存储芯片用单组分环氧胶粘剂的研制

    Institute of Scientific and Technical Information of China (English)

    戚渭新

    2002-01-01

    A method of making a one-package epoxy resin sealant for electronic memory chips and the characteristics of the sealant were presented.. The process and the conditions of preparing the latent curing agent were discussed in detail. The principle of preparing the curing agent is to lower reactivity of a heterocyclic amine and envelop it simultaneously.This sealant can cure rapidly and completely in the condition of 110 ℃ and 30 min, and it is stable for storage at 20 ℃. Its application showed that the sealant can take the place of the imported sealant.%介绍了电子存储芯片用密封用单组分环氧胶粘剂的制备方法及产品特点,详细讨论了潜伏性固化剂的制备条件,本固化剂采用杂环胺减活性同时进行包裹的方法制备,110 ℃×30min可固化,20 ℃可贮存6个月,使用结果证明可以取代进口胶.

  13. Four-Group Classification Based on Fibrinogen Level and Fibrin Polymerization Associated With Postoperative Bleeding in Cardiac Surgery.

    Science.gov (United States)

    Kawashima, Shingo; Suzuki, Yuji; Sato, Tsunehisa; Kikura, Mutsuhito; Katoh, Takasumi; Sato, Shigehito

    2016-10-01

    Fibrinogen and fibrin formation have a key role in perioperative hemostasis. The aim of this study is to examine the association of postoperative hemostasis with a combined evaluation of the fibrinogen level and fibrin polymerization in cardiac surgery. We retrospectively classified 215 consecutive cardiac surgery patients into 4 groups (Fuji-san classification) that were divided by fibrinogen level fibrinogen thromboelastometry value at 10 minutes with rotational thromboelastometry <6 mm (ie, low fibrin polymerization) at the warming of cardiopulmonary bypass. Four groups resulted; group I, the acceptable range (n = 85); group II, only hypofibrinogenemia (<150 mg/dL, ≥6 mm, n = 63); group III, hypofibrinogenemia and low fibrin polymerization (<150 mg/dL, <6 mm, n = 60); and group IV, only low fibrin polymerization (≥150 mg/dL, <6 mm, n = 7). The risk of chest tube drainage volume greater than 500 mL within the first 24 hours after surgery (with group I as the reference) was increased in group II (odds ratio [OR], 3.3; 95% confidence interval [CI], 1.5-7.4; P < .01) and group III (OR, 8.5; 95% CI, 3.5-21.7; P < .01), and the risk greater than 1000 mL (with group I as the reference) was increased in group III (OR, 4.0; 95% CI, 1.1-17.3; P = .03) and group IV (OR, 23.1; 95% CI, 3.2-201.0; P < .01). Intraoperative blood transfusions were decreased by 24.5%, after stratifying the starting amount of fresh frozen plasma by the 4-group classification in the recent consecutive 65 (30.2%) patients (P < .01). The 4-group classification is associated with postoperative bleeding and may improve the quality of perioperative blood transfusion in cardiac surgery.

  14. An essential primer for understanding the role of topical hemostats, surgical sealants, and adhesives for maintaining hemostasis.

    Science.gov (United States)

    Gabay, Michael; Boucher, Bradley A

    2013-09-01

    A wide variety of topical hemostats are approved as adjunctive therapies in the maintenance of hemostasis during surgical procedures in which conventional methods are insufficient or not practical. A multidisciplinary approach to the selection and application of these agents requires input from all members of the surgical team including surgeons, perioperative nurses, blood bank specialists, and pharmacists. However, pharmacist knowledge regarding topical hemostats may be limited based on lack of formal education within college of pharmacy curricula as well as their use being predominantly in the operating room setting. Furthermore, some of these agents might be procured through central supply rather than the hospital pharmacy. Topical hemostats include agents that act as a mechanical barrier to bleeding and provide a physical matrix for clotting, biologically active agents that catalyze coagulation, combination therapies, and synthetic sealants and adhesives. Although many of the topical hemostats were approved for use before the requirement for clinical trials, this review provides an overview of the available clinical evidence regarding the appropriate uses and safety considerations associated with these agents. Proper use of these agents is vital to achieving the best clinical outcomes. Specifically, knowledge of the contraindications and potential adverse events associated with topical hemostats can help prevent unwanted outcomes. Therefore, an understanding of the benefits and potential risks associated with these agents will allow hospital pharmacists to assist in the development and implementation of institutional policies regarding the safe and effective use of hemostatic agents commonly used in the surgical suite.

  15. Integration of colloids into a semi-flexible network of fibrin.

    Science.gov (United States)

    Bharadwaj, N Ashwin K; Kang, Jin Gu; Hatzell, Marta C; Schweizer, Kenneth S; Braun, Paul V; Ewoldt, Randy H

    2017-02-15

    Typical colloid-polymer composites have particle diameters much larger than the polymer mesh size, but successful integration of smaller colloids into a large-mesh network could allow for the realization of new colloidal states of spatial organization and faster colloid motion which can allow the possibility of switchable re-configuration of colloids or more dramatic stimuli-responsive property changes. Experimental realization of such composites requires solving non-trivial materials selection and fabrication challenges; key questions include composition regime maps of successful composites, the resulting structure and colloidal contact network, and the mechanical properties, in particular the ability to form a network and retain strain stiffening in the presence of colloids. Here, we study these fundamental questions by formulating composites with fluorescent (though not stimuli-responsive) carboxylate modified polystyrene/latex (CML) colloidal particles (diameters 200 nm and 1000 nm) in bovine fibrin networks (a semi-flexible biopolymer network with mesh size 1-5 μm). We describe and characterize two methods of composite preparation: adding colloids before fibrinogen polymerization (Method I), and electrophoretically driving colloids into a network already formed by fibrinogen polymerization (Method II). We directly image the morphology of colloidal and fibrous components with two-color fluorescent confocal microscopy under wet conditions and SEM of fixed dry samples. Mechanical properties are studied with shear and extensional rheology. Both fabrication methods are successful, though with trade-offs. Method I retains the nonlinear strain-stiffening and extensibility of the native fibrin network, but some colloid clustering is observed and fibrin network integrity is lost above a critical colloid concentration that depends on fibrinogen and thrombin concentration. Larger colloids can be included at higher volume fractions before massive aggregation occurs

  16. Genetic predictors of fibrin D-dimer levels in healthy adults

    Science.gov (United States)

    Smith, Nicholas L.; Huffman, Jennifer E.; Strachan, David P.; Huang, Jie; Dehghan, Abbas; Trompet, Stella; Lopez, Lorna M.; Shin, So-Youn; Baumert, Jens; Vitart, Veronique; Bis, Joshua C.; Wild, Sarah H.; Rumley, Ann; Yang, Qiong; Uitterlinden, Andre G; Stott, David. J.; Davies, Gail; Carter, Angela M.; Thorand, Barbara; Polašek, Ozren; McKnight, Barbara; Campbell, Harry; Rudnicka, Alicja R.; Chen, Ming-Huei; Buckley, Brendan M.; Harris, Sarah E.; Williams, Frances M. K.; Peters, Annette; Pulanic, Drazen; Lumley, Thomas; de Craen, Anton J.M.; Liewald, David C.; Gieger, Christian; Campbell, Susan; Ford, Ian; Gow, Alan J.; Luciano, Michelle; Porteous, David J.; Guo, Xiuqing; Sattar, Naveed; Tenesa, Albert; Cushman, Mary; Slagboom, P. Eline; Visscher, Peter M.; Spector, Tim D.; Illig, Thomas; Rudan, Igor; Bovill, Edwin G.; Wright, Alan F.; McArdle, Wendy L.; Tofler, Geoffrey; Hofman, Albert; Westendorp, Rudi G.J.; Starr, John M.; Grant, Peter J.; Karakas, Mahir; Hastie, Nicholas D.; Psaty, Bruce M.; Wilson, James F.; Lowe, Gordon D. O.; O’Donnell, Christopher J; Witteman, Jacqueline CM; Jukema, J. Wouter; Deary, Ian J.; Soranzo, Nicole; Koenig, Wolfgang; Hayward, Caroline

    2011-01-01

    Background Fibrin fragment D-dimer is one of several peptides produced when cross-linked fibrin is degraded by plasmin, and is the most widely-used clinical marker of activated blood coagulation. To identity genetic loci influencing D-dimer levels, we performed the first large-scale, genome-wide association search. Methods and Results A genome-wide investigation of the genomic correlates of plasma D-dimer levels was conducted among 21,052 European-ancestry adults. Plasma levels of D-dimer were measured independently in each of 13 cohorts. Each study analyzed the association between ~2.6 million genotyped and imputed variants across the 22 autosomal chromosomes and natural-log transformed D-dimer levels using linear regression in additive genetic models adjusted for age and sex. Among all variants, 74 exceeded the genome-wide significance threshold and marked 3 regions. At 1p22, rs12029080 (p-value 6.4×10−52) was 46.0 kb upstream from F3, coagulation factor III (tissue factor). At 1q24, rs6687813 (p-value 2.4×10−14) was 79.7 kb downstream of F5, coagulation factor V. At 4q32, rs13109457 (p-value 2.9×10−18) was located between 2 fibrinogen genes: 10.4 kb downstream from FGG and 3.0 kb upstream from FGA. Variants were associated with a 0.099, 0.096, and 0.061 unit difference, respectively, in natural-log transformed D-dimer and together accounted for 1.8% of the total variance. When adjusted for non-synonymous substitutions in F5 and FGA loci known to be associated with D-dimer levels, there was no evidence of an additional association at either locus. Conclusions Three genes were associated with fibrin D-dimer levels, of which the F3 association was the strongest and has not been previously reported. PMID:21502573

  17. Revascularization of immature, nonvital permanent tooth using platelet-rich fibrin in children.

    Science.gov (United States)

    Nagaveni, N B; Poornima, P; Joshi, Jooie S; Pathak, Sidhant; Nandini, D B

    2015-01-01

    The purpose of this paper was to present a new approach wherein revascularization of the immature, nonvital permanent tooth was performed using platelet-rich fibrin (PRF) as a novel scaffold material. This was performed after disinfection of the root canal space using triple antibiotic paste followed by placing a PRF membrane in the root canal. The patient was followed up regularly at three-, six-, nine-, and 12-month intervals for review. After 12 months, clinical examination showed negative response to percussion and palpation tests but positive response to cold and electric pulp tests. Radiographic examination revealed continued thickening of the root dentinal walls, narrowing of root canal space, root lengthening, and closure of the root apex with normal periradicular architecture. However, more clinical research using large samples is necessary to prove it advantageous for regenerative endodontic therapy in children.

  18. Tulip bundle technique and fibrin glue injection:Unusual treatment of colonic perforation

    Institute of Scientific and Technical Information of China (English)

    Filippo Mocciaro; Gabriele Curcio; Ilaria Tarantino; Luca Barresi; Marco Spada; Sergio Li Petri; Mario Traina

    2011-01-01

    We report a case of a 63-year-old male who experienced an iatrogenic sigmoid perforation repaired combining three endoscopic techniques.The lesion was large and irregular with three discrete perforations, therefore,we decided to close it by placing one clip per perforation,and then connecting all the clips with two endoloops.Finally we chose to use a fibrin glue injection to obtain a complete sealing.Four days after the colonoscopy the patient underwent a laparoscopic right hemicolectomy due to evidence of a large polyp of the caecum with high grade dysplasia and focal carcinoma in situ .Inspection of the sigma showed complete repair of the perforation.This report underlines how a conservative approach,together with a combination of various endoscopic techniques,can resolve complicated iatrogenic perforations of the colon.

  19. Tissue factor pathway inhibitor relates to fibrin degradation in patients with acute deep venous thrombosis

    DEFF Research Database (Denmark)

    Sidelmann, Johannes J; Bladbjerg, Else-Marie; Gram, Jørgen

    2008-01-01

    Reduced concentration of tissue factor pathway inhibitor is a risk factor for development of deep venous thrombosis, whereas elevated concentrations of tissue factor pathway inhibitor are observed in patients with acute myocardial infarction and disseminated intravascular coagulation. Presently, we...... studied the association between inflammation, endothelial cell perturbation, fibrin degradation and the concentration of tissue factor pathway inhibitor in patients suspected for acute deep venous thrombosis. We determined the tissue factor pathway inhibitor -33T/C polymorphism, free and total tissue...... factor pathway inhibitor, C-reactive protein, von Willebrand factor and D-Dimer in 160 consecutive patients admitted to hospital with a tentative diagnosis of acute deep venous thrombosis. Deep venous thrombosis was identified in 57 patients (18 distal and 39 proximal). The distribution of the tissue...

  20. Clinico-pathological analysis referring hemeoxygenase-1 in acute fibrinous and organizing pneumonia patients

    Directory of Open Access Journals (Sweden)

    Yu Hara

    2015-01-01

    We experienced two cases of subacute form AFOP for these 10 years and reviewed clinico-pathological characteristics. The average age was 62 years old and both were male. The etiology of both cases was idiopathic. The average PaO2/FIO2 ratio was 274.5 ± 84.1. The average levels of C-reactive protein and surfactant protein - A of the serum were elevated to 19.8 ± 6.3 mg/dL and 67.6 ± 15.8 ng/mL, respectively. Serum sialylated carbohydrate antigen levels were normal in both cases. The characteristic radiographic findings were bilateral consolidations and ground glass opacities. Lung biopsy specimens revealed fibrin balls and alveolitis with abundant cellular HO-1 expression. Steroid response was excellent and the pulmonary involvements absolutely disappeared for about 3 months.

  1. A simple application technique of fibrin-coated collagen fleece (TachoComb) in laparoscopic surgery.

    Science.gov (United States)

    Nakajima, Kiyokazu; Yasumasa, Keigo; Endo, Shunji; Takahashi, Tsuyoshi; Kai, Yasuyuki; Nezu, Riichiro; Nishida, Toshirou

    2007-01-01

    A fibrin-coated collagen fleece (TachoComb, Nycomed, Denmark) is a powerful topical hemostatic agent, which has been aggressively used in conventional open surgery with a favorable clinical outcome. However, the use of TachoComb in laparoscopic surgery has not yet gained wide clinical acceptance, because a simple and well-functioning application system is not available. The authors have newly developed a quick, simple, and effective laparoscopic TachoComb application technique: housing a small strip of TachoComb in a rubber tube, then conveying it into the peritoneal cavity, and applying it using standard laparoscopic forceps. The repeated application of TachoComb strips is feasible and of practical value especially in laparoscopic surgery, since a small TachoComb never compromises either the application procedure or laparoscopic visualization.

  2. Biomimetic interconnected porous keratin-fibrin-gelatin 3D sponge for tissue engineering application.

    Science.gov (United States)

    Singaravelu, Sivakumar; Ramanathan, Giriprasath; Raja, M D; Nagiah, Naveen; Padmapriya, P; Kaveri, Krishnasamy; Sivagnanam, Uma Tiruchirapalli

    2016-05-01

    The medicated wound dressing material with highly interconnected pores, mimicking the function of the extracellular matrix was fabricated for the promotion of cell growth. In this study, keratin (K), fibrin (F) and gelatin (G) composite scaffold (KFG-SPG) was fabricated by freeze drying technique and the mupirocin (D) drug was successfully incorporated with KFG-SPG (KFG-SPG-D) intended for tissue engineering applications. The fabrication of scaffold was performed without the use of any strong chemical solvents, and the solid sponge scaffold was obtained with well interconnected pores. The porous morphology of the scaffold was confirmed by SEM analysis and exhibited competent mechanical properties. KFG-SPG and KFG-SPG-D possess high level of biocompatibility, cell proliferation and cell adhesion of NIH 3T3 fibroblast and human keratinocytes (HaCaT) cell lines thereby indicating the scaffolds potential as a suitable medicated dressing for wound healing.

  3. Fibrin in Reproductive Tissue Engineering: A Review on Its Application as a Biomaterial for Fertility Preservation.

    Science.gov (United States)

    Chiti, M C; Dolmans, M M; Donnez, J; Amorim, C A

    2017-03-07

    In recent years, reproductive medicine has made good use of tissue engineering and regenerative medicine techniques to develop alternatives to restore fertility in cancer patients. For young female cancer patients who cannot undergo any of the currently applied strategies due to the possible presence of malignant cells in their ovaries, the challenge is creating an in vitro or in vivo artificial ovary using carefully selected biomaterials. Thanks to its numerous qualities, fibrin has been widely used as a scaffold material for fertility preservation applications. The goal of this review is to examine and discuss the applications and advantages of this biopolymer for fertility restoration in cancer patients, and consider the main results achieved so far.

  4. Changes in fibrin D-dimer, fibrinogen, and protein S during pregnancy

    DEFF Research Database (Denmark)

    Hansen, Anette Tarp; Andreasen, Birgitte Horst; Salvig, Jannie Dalby

    2010-01-01

    Background. Pregnancy is a hypercoagulable state with a 5- to 10- fold higher risk of venous thromboembolism. Existing reference intervals for fibrin D-dimer (D-dimer), functional fibrinogen (fibrinogen) and protein S, free antigen (protein S) are based on non-pregnant patients and reference...... intervals for pregnant patients are warranted. Objectives. The aim of the present study was to contribute to the establishment of reference intervals for D-dimer, fibrinogen and protein S during pregnancy and to discuss the use of the analyses during pregnancy. Methods. We included 55 healthy pregnant women...... in gestational week 11–17, with normal current pregnancy. Blood samples were collected in gestational weeks 11–17, 21–27 and 34–37. The three plasma parameters D-dimer, fibrinogen and protein S were analysed by STA-R Evolution®. Results. A significant rise in D-dimer was found from first to second trimester (p...

  5. DIAGNOSIS AND MEDICAL AND SURGICAL MANAGEMENT OF CHRONIC INFECTIOUS FIBRINOUS PLEURITIS IN AN OKAPI (OKAPIA JOHNSTONI).

    Science.gov (United States)

    Franzen, Dana; Lamberski, Nadine; Zuba, Jeffery; Richardson, G Lynn; Fischer, A T; Rantanen, Norman W

    2015-06-01

    A 10-yr-old female okapi (Okapia johnstoni) at the San Diego Zoo Safari Park was evaluated for intermittent malaise, inappetence, occasional cough, abdominal splinting, and licking at both flanks. Physical examination revealed tachypnea, tachycardia, and fluid sounds on thoracic auscultation. Transthoracic ultrasound showed multiple uniform, anechoic filled structures in the right and left pleural space. Surgical exploration of the thoracic cavity revealed bilateral, mature, fibrous, compartmentalizing adhesions between the visceral and parietal pleura, confirming a diagnosis of chronic, infectious, fibrinous pleuritis. The suspected etiology was occult aspiration pneumonia secondary to historical episodes of regurgitation associated with general anesthesia. Culture of the pleural fluid and fibrous adhesions grew Trueperella (Arcanobacterium) pyogenes, Arcanobacterium haemolyticum, and few Fusobacterium species. Treatment consisted of chest-tube placement to establish drainage, thoracic lavage, unilateral surgical debridement, and long-term antibiotics. The animal made a complete clinical recovery over 7 mo.

  6. A New Potential Cause in the Development of Toxic Anterior Segment Syndrome: Fibrin Glue

    Directory of Open Access Journals (Sweden)

    Selçuk Sızmaz

    2014-08-01

    Full Text Available Objectives: To present a potential cause for toxic anterior segment syndrome (TASS. Materials and Methods: We report 4 cases of TASS that occurred following uneventful phacoemulsification and intraocular lens implantation. Results: The 4 cases were the first consecutive 2 cases of 2 different surgery days, 5 months apart. The most prominent sign of TASS was limbus-to-limbus corneal edema. Pain and/or intraocular pressure rise were also common. All surgical and presurgical procedures were checked after the first outbreak, whereas the second outbreak required further investigation. Fibrin glue remnants from preceding pterygium surgery with conjunctival autografting were found to be the potential cause. Despite intensive corticosteroid therapy, corneal edema did not resolve in 2 patients who underwent keratoplasty. Conclusion: TASS is a sight-threatening condition which requires thorough investigation for prevention of new cases. All steps must be carefully revised. (Turk J Ophthalmol 2014; 44: 280-3

  7. Treatment of infrabony defects with platelet-rich fibrin along with bone graft: Case report

    Directory of Open Access Journals (Sweden)

    Surekha Y Bhedasgaonkar

    2015-01-01

    Full Text Available Although periodontitis is an infectious disease of periodontal tissues, changes that occur in the bone are crucial, because destruction of bone is responsible for tooth loss. Although horizontal bone loss is most common, vertical bone loss is more amenable to regenerative periodontal therapy. Recently, importance has been given to use of platelet-rich fibrin (PRF for predictably obtaining periodontal regeneration. PRF is a concentrated suspension of growth factors found in platelets. These growth factors moderate the wound healing and promote tissue regeneration. This article includes case reports of two patients who showed infrabony defects that were treated with combining PRF with allograft. This case report tried to compare the clinical and radiographical outcome obtained by combination of PRF and allograft.

  8. Time-dependent biodistribution, clearance and biocompatibility of magnetic fibrin nanoparticles: an in vivo study

    Science.gov (United States)

    Prabu, Periyathambi; Vedakumari, Weslen S.; Sastry, Thotapalli P.

    2015-05-01

    Recently, bioretention and toxicity of injected nanoparticles in the body has drawn much attention in biomedical research. In the present study, 5 mg Fe per kg body weight of magnetic fibrin nanoparticles (MFNPs) were injected into mice intravenously and investigated for their blood clearance profile, biodistribution, haematology and pathology studies for a time period of 28 days. Moderately long circulation of MFNPs in blood was observed with probable degradation and excretion into the bloodstream via monoatomic iron forms. Inductively coupled plasma optical emission spectrometry (ICP-OES) and Prussian blue staining results showed increased accumulation of MFNPs in the liver, followed by spleen and other organs. Body weight, spleen/thymus indexes, haematology, serum biochemistry and histopathology studies demonstrated that MFNPs were biocompatible. These results suggest the feasibility of using MFNPs for drug delivery and imaging applications.

  9. Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel.

    Science.gov (United States)

    Xu, Yufan; Wang, Xiaohong

    2015-08-01

    Three-dimensional (3D) cell manipulation is available with the integration of microfluidic technology and rapid prototyping techniques. High-Fidelity (Hi-Fi) constructs hold enormous therapeutic potential for organ manufacturing and regenerative medicine. In the present paper we introduced a quasi-three-dimensional (Q3D) model with parallel biocompatible alginate/gelatin/fibrin hurdles. The behaviors of fluids and cells along the microfluidic channels with various widths were studied. Cells inside the newly designed microfluidic channels attached and grew well. Morphological changes of adipose-derived stem cells (ADSCs) in both two-dimensional (2D) and 3D milieu were found on the printed constructs. Endothelialization occurred with the co-cultures of ADSCs and hepatocytes. This study provides insights into the interactions among fluids, cells and biomaterials, the behaviors of fluids and cells along the microfluidic channels, and the applications of Q3D techniques.

  10. Efficacy of a root conditioning agent on fibrin network formation in periodontal regeneration: A SEM evaluation

    Directory of Open Access Journals (Sweden)

    Rajender Amireddy

    2011-01-01

    Full Text Available Background: Even though numerous biomaterials have been devised and employed for periodontal regeneration, it should be well understood that the root surface receptiveness to clot formation and maintenance during initial periodontal wound healing, decides the nature of the connective tissue attachment. So this study was carried out with the prime objective of assessing the initial wound healing events occurring in vivo after the application of citric acid on to the root surfaces during periodontal regenerative therapy. Materials and Methods: Thirty-two human teeth were used for this in vitro study. Two dentin blocks each measuring 4 × 2 × 1 mm were made from each tooth. These dentin blocks were planed and treated differently with Phosphate Buffered Saline (PBS, citric acid, PBS and fresh human blood, citric acid and fresh human blood and were segregated into eight groups. Finally all the dentin blocks were processed and subjected to a scanning electron microscope study. Results: In PBS-treated samples, the dentin surface was irregular corresponding to smear layer and the dentinal tubule openings were obscured. Whereas, in those treated with citric acid revealed a smooth dentin surface devoid of smear layer and the dentinal tubular openings were clear. Further samples that were treated with PBS plus blood showed little or no fibrin network formation whereas with those citric acid plus blood showed a fine thick fibrin network formation adhered to dentinal surface. Conclusion: The results of this present in vitro study suggests that use of citric acid as a root conditioning agent has a beneficial effect on initial wound healing events, which are critical for periodontal regenerative therapies.

  11. Association of Fibrin Monomer Polymerization Function, Cerebrovascular Risk Factors and Ischemic Cerebrovascular Disease in Old People

    Institute of Scientific and Technical Information of China (English)

    洪梅; 魏文宁; 李红戈; 杨锐; 杨焰

    2003-01-01

    Summary: In order to investigate the association of fibrin monomer polymerization function (FMPF)with traditional cerebrovascular risk factors and ischemic cerebrovascular disease in old people, 1 : 1paired case-control comparative study was performed for FMPF and traditional cerebrovascular riskfactors on 110 cases of old ischemic cerebrovascular disease and 110 controls matched on age, sex andliving condition. The results showed that cerebrovascular risk factors were more prevalent in casegroup than in control group. In the case group, FMPF was significantly higher than in controlgroup. There was a significant positive correlation between hypertension and fibrin monomer poly-merization velocity (FMPV), hypertension and fibrinogen (Fbg), alcohol consumption and Fbg, butno significant correlation between diabetic mellitus, smoking and FMPF was found. Among the pa-rameters of blood lipids, there were significant positive correlations between total cholesterol (TC)and parameters of FMPF to varying degrees, triglycerides (TG) and FMPV, TG and Fbg. Our re-sults also showed there were significant linear trends between TC and FMPV (P<0. 001), TC andFbg (P=0. 0087), TG and FMPV/Amax (maximum absorbance)(P=0. 0143) respectively. Multi-ple logistic regression analysis revealed that FMPF in case group remained significantly higher thancontrol group after adjustment of all risk factors that were significant in univariate analysis. It wasconcluded that there is a possible pathophysiological link between FMPF and cerebrovascular risk fac-tors. An elevated FMPF is associated with ischemic cerebrovascular disease and an independent riskfactor of this disease. In old people, detection of FMPF might be a useful screening to identify indi-viduals at increased cerebrothrombotic risk.

  12. Modeling of fibrin gels based on confocal microscopy and light-scattering data.

    Science.gov (United States)

    Magatti, Davide; Molteni, Matteo; Cardinali, Barbara; Rocco, Mattia; Ferri, Fabio

    2013-03-01

    Fibrin gels are biological networks that play a fundamental role in blood coagulation and other patho/physiological processes, such as thrombosis and cancer. Electron and confocal microscopies show a collection of fibers that are relatively monodisperse in diameter, not uniformly distributed, and connected at nodal points with a branching order of ∼3-4. Although in the confocal images the hydrated fibers appear to be quite straight (mass fractal dimension D(m) = 1), for the overall system 1, joined at randomly distributed nodal points. The resulting 3D network strikingly resembles real fibrin gels and can be sketched as an assembly of densely packed fractal blobs, i.e., regions of size ξ, where the fiber concentration is higher than average. The blobs are placed at a distance ξ0 between their centers of mass so that they are overlapped by a factor η =ξ/ξ0 and have D(m) ∼1.2-1.6. The in silico gels' structure is quantitatively analyzed by its 3D spatial correlation function g(3D)(r) and corresponding power spectrum I(q) = FFT(3D[g3D(r)]), from which ρ, d, D(m), η, and ξ0 can be extracted. In particular, ξ0 provides an excellent estimate of the gel mesh size. The in silico gels' I(q) compares quite well with real gels' elastic light-scattering measurements. We then derived an analytical form factor for accurately fitting the scattering data, which allowed us to directly recover the gels' structural parameters.

  13. Platelet-rich fibrin-mediated revitalization of immature necrotic tooth

    Directory of Open Access Journals (Sweden)

    Navin Mishra

    2013-01-01

    Full Text Available Contemporary studies have shown that the regeneration of tissues and root elongation is possible in necrotic immature permanent teeth. The purpose of this case report is to add a new vista in regenerative endodontic therapy by using platelet rich fibrin for revitalization of immature non vital tooth. An 11year old boy with the history of trauma was diagnosed with the pulpal necrosis and symptomatic apical periodontitis in tooth #21. Intra oral periapical radiograph showed open apex and associated immature supernumerary tooth with respect to tooth #21. Access preparation and minimal instrumentation was done to remove necrotic debris under copious irrigation with 2.5% sodium hypochlorite. Triple antibiotic paste was packed in the canal for four weeks. During second visit, 5 mL of whole blood was drawn from the medial cubital vein of the patient and blood was then subjected to centrifugation at 2400 rpm for 12 minutes for the preparation of Platelet rich fibrin (PRF utilizing Choukroun′s method. Triple antibiotic paste was removed and canal was dried. PRF clot was pushed to the apical region of tooth #21 using hand pluggers. Three milimetres of Mineral trioxide (MTA was placed in cervical part of the root canal and permanent restoration was done three days later. Clinical examination at 6 and 12 months revealed no sensitivity to percussion and palpation in tooth #21and it responded positively to both electric pulp and cold tests. Radiographic examination showed resolution of periapical rarefaction, further root development and apical closure of the tooth #21 and its associated supernumerary tooth. On the basis of successful outcome of the present case it can be stated that PRF clot may serve as a scaffold for regeneration of necrotic immature teeth.

  14. Posıtıve effect of platelet rich fibrin on osseointegration

    Science.gov (United States)

    Bayram, Burak; Kantarcı, Alpdoğan; Gülsever, Serap; Alaaddinoğlu, Emine-Elif

    2016-01-01

    Background Leukocyte-platelet rich fibrin (L-PRF) is a second generation platelet concentrate clinically used to accelerate tissue healing and bone regeneration. Achieving reduced implant osseointegration time could provide immediate or early loading of implants. The aim of this study was to evaluate the L-PRF-induced osseointegration and bone-implant contact (BIC) in an experimental animal model. Material and Methods Twelve 4-month-old New Zealand white rabbits were used. Following general anesthesia, 3-5 mL of blood was obtained from the central artery in rabbit ear and L-PRF was prepared. Two implant cavities (5 mm long and 3 mm in diameter) were created in each tibia with a total of four cavities in each animal. Two of these cavities were selected and covered with PRF (test group). The remaining L-PRF was used to soak the implants placed into the L-PRF covered sockets. Other cavities were left as controls. In total, 48 implants were placed. Animals were sacrificed after two, three, or four weeks. Histological samples were obtained and peri-implant tissues were histomorphometrically evaluated for bone-to-implant contact and new bone formation. Results Histomorphometric analyses of the defects revealed that the L-PRF was detectable up to the second week. Application of L-PRF increased the rate and amount of new bone formation in the experimental group compared to the control group. Bone-to-implant contact was enhanced when the surface was pre-wetted with L-PRF (p<0.01). Conclusions The results of this study demonstrated that L-PRF application may increases amount and rate of new bone formation during the early healing period and provides a faster osseointegration around implants. Key words:Dental implants, platelet rich fibrin, osseointegration, bone regeneration, matrix for growth factors. PMID:27475686

  15. Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial

    DEFF Research Database (Denmark)

    Danielsen, P.; Jorgensen, B.; Jorgensen, L.N.;

    2008-01-01

    BACKGROUND: Autologous platelet-rich fibrin contains multiple growth factors. The aim of this randomized clinical trial was to study the effect of topical platelet-rich fibrin on epithelialization of donor sites and meshed split-thickness skin autografts. METHODS: Twenty consecutive leg ulcer...... surgically revised leg ulcer were randomized by concealed allocation to platelet-rich fibrin, and the other donor wound and autografted half were not (control). Biopsy specimens (4 mm) from donor wounds were evaluated for percentage neoepidermal coverage in tissue sections immunostained for keratin on days 5...... and 8. Epithelial barrier function, macroscopic healing, microbiology, and pain at dressing removal were assessed. Epithelialization of meshed autografts was assessed macroscopically. RESULTS: Epithelial coverage of donor wounds did not differ significantly between platelet-rich fibrin and control...

  16. Resistência ao cisalhamento de um selante associado a componentes de um sistema adesivo dental Shear bond strength of an enamel sealant using components of a dental adhesive system

    Directory of Open Access Journals (Sweden)

    Zilda Maria MUSSOLINO

    1998-10-01

    Full Text Available Cilindros de selante, padronizados, foram unidos ao esmalte das superfícies vestibulares de incisivos bovinos, após serem planificadas e condicionadas, com ácido fosfórico a 37%, durante 30 segundos. Foram utilizados 40 dentes, aleatoriamente divididos em 4 grupos, cujas coroas foram secionadas, de modo a obter-se duas porções, cervical e incisal. No Grupo I, após o condicionamento, aplicou-se o selante Fluroshield; no Grupo II, antes da aplicação do selante, uma camada do "primer" do Probond foi aplicada; no Grupo III, após o "primer", aplicou-se o adesivo do Probond; e no Grupo IV, somente o adesivo foi aplicado antes do selante. Os espécimes foram armazenados em água a 37°C, durante 36 horas, e então submetidos aos ensaios de cisalhamento. A análise estatística revelou significante diminuição na resistência ao cisalhamento, quando o "primer" foi aplicado previamente ao selante, enquanto a resistência ao cisalhamento do selante foi semelhante quando o adesivo do Probond foi aplicado, com ou sem o "primer". A resistência ao cisalhamento do selante ao esmalte é maior no terço incisal que no terço cervical da coroa.Standardized cylinders of sealants were bonded to the flattened labial enamel of bovine incisor teeth that had previously been subjected to 37% phosphoric acid gel for 30 seconds. A total of 40 teeth were tested, randomly divided in four groups of 10 teeth each. In Group I, the sealant Fluroshield was applied after etching; in Group II after etching, the "primer" of Probond was used before the sealant; in Group III after etching, the bond of Probond was used after the "primer"; and in Group IV only the bond was applied before the sealant. Specimens were stored in water at 37°C during 36 hours, before shear testing using a Universal Testing Machine. There was significant reduction in shear bond strength of the sealant when only the "primer" was used previously to the application of the sealant. There were no

  17. Leakage diagnostics, sealant longevity, sizing and technologytransfer in residential thermal distribution systems: Part II.Residential thermal Distribution Systesm, Phase VI FinalReport

    Energy Technology Data Exchange (ETDEWEB)

    Buchanan, C.; Modera, M.; Sherman, M.; Siegel, J.; Walker, I.; Wang, D.

    1998-12-01

    This report builds on and extends our previous efforts as described in "Leakage Diagnostics, Sealant Longevity, Sizing and Technology Transfer in Residential Thermal Distribution Systems- CIEE Residential Thermal Distribution Systems Phase V Final Report, October 1997". New developments include defining combined duct and equipment efficiencies in a concept called "Tons At the Register" and on performance issues related to field use of the aerosol sealant technology. Some of the key results discussed in this report include: o Register, boot and air handler cabinet leakage can often represent a significant fraction of the total duct leakage in new construction. Because of the large range of pressures in duct systems an accurate characterization may require separating these components through improved leakage testing. o Conventional duct tape failed our accelerated longevity testing and is not, therefore, considered generally acceptable for use in sealing duct systems. Many other tapes and sealing approaches are available and practical and have passed our longevity tests. o Simulations of summer temperature pull-down time have shown that duct system improvements can be combined with equipment downsizing to save first cost, energy consumption, and peak power and still provide equivalent or superior comfort. o Air conditioner name plate capacity ratings alone are a poor indicator of how much cooling will actually be delivered to the conditioned space. Duct system efficiency can have as large an impact on performance as variations in SEER. o Mechanical duct cleaning techniques do not have an adverse impact on the ducts sealed with the Aerosol sealant. The material typically used in Aerosol sealing techniques does not appear to present a health or safety hazard. Results from this study were used by the California Energy Commission in the formation of the current Energy Efficiency Standards for Low-Rise Residential Buildings (CEC, (1998)), often referred to as Title 24

  18. Characterization of Fast-Scan Cyclic Voltammetric Electrodes Using Paraffin as an Effective Sealant with In Vitro and In Vivo Applications.

    Directory of Open Access Journals (Sweden)

    Eric S Ramsson

    Full Text Available Fast-scan cyclic voltammetry (FSCV is a powerful technique for measuring sub-second changes in neurotransmitter levels. A great time-limiting factor in the use of FSCV is the production of high-quality recording electrodes; common recording electrodes consist of cylindrical carbon fiber encased in borosilicate glass. When the borosilicate is heated and pulled, the molten glass ideally forms a tight seal around the carbon fiber cylinder. It is often difficult, however, to guarantee a perfect seal between the glass and carbon. Indeed, much of the time spent creating electrodes is in an effort to find a good seal. Even though epoxy resins can be useful in this regard, they are irreversible (seals are permanent, wasteful (epoxy cannot be reused once hardener is added, hazardous (hardeners are often caustic, and require curing. Herein we characterize paraffin as an electrode sealant for FSCV microelectrodes. Paraffin boasts the advantages of near-immediate curing times, simplicity in use, long shelf-life and stable waterproof seals capable of withstanding extended cycling. Borosilicate electrode tips were left intact or broken and dipped in paraffin embedding wax. Excess wax was removed from the carbon surface with xyelenes or by repeated cycling at an extended waveform (-0.4 to 1.4V, 400 V/s, 60 Hz. Then, the waveform was switched to a standard waveform (-0.4 to 1.3V, 400 V/s, 10 Hz and cycled until stable. Wax-sealing does not inhibit electrode sensitivity, as electrodes detected linear changes in dopamine before and after wax (then xylenes exposure. Paraffin seals are intact after 11 days of implantation in the mouse, and still capable of measuring transient changes in in vivo dopamine. From this it is clear that paraffin wax is an effective sealant for FSCV electrodes that provides a convenient substitute to epoxy sealants.

  19. Characterization of Fast-Scan Cyclic Voltammetric Electrodes Using Paraffin as an Effective Sealant with In Vitro and In Vivo Applications.

    Science.gov (United States)

    Ramsson, Eric S; Cholger, Daniel; Dionise, Albert; Poirier, Nicholas; Andrus, Avery; Curtiss, Randi

    2015-01-01

    Fast-scan cyclic voltammetry (FSCV) is a powerful technique for measuring sub-second changes in neurotransmitter levels. A great time-limiting factor in the use of FSCV is the production of high-quality recording electrodes; common recording electrodes consist of cylindrical carbon fiber encased in borosilicate glass. When the borosilicate is heated and pulled, the molten glass ideally forms a tight seal around the carbon fiber cylinder. It is often difficult, however, to guarantee a perfect seal between the glass and carbon. Indeed, much of the time spent creating electrodes is in an effort to find a good seal. Even though epoxy resins can be useful in this regard, they are irreversible (seals are permanent), wasteful (epoxy cannot be reused once hardener is added), hazardous (hardeners are often caustic), and require curing. Herein we characterize paraffin as an electrode sealant for FSCV microelectrodes. Paraffin boasts the advantages of near-immediate curing times, simplicity in use, long shelf-life and stable waterproof seals capable of withstanding extended cycling. Borosilicate electrode tips were left intact or broken and dipped in paraffin embedding wax. Excess wax was removed from the carbon surface with xyelenes or by repeated cycling at an extended waveform (-0.4 to 1.4V, 400 V/s, 60 Hz). Then, the waveform was switched to a standard waveform (-0.4 to 1.3V, 400 V/s, 10 Hz) and cycled until stable. Wax-sealing does not inhibit electrode sensitivity, as electrodes detected linear changes in dopamine before and after wax (then xylenes) exposure. Paraffin seals are intact after 11 days of implantation in the mouse, and still capable of measuring transient changes in in vivo dopamine. From this it is clear that paraffin wax is an effective sealant for FSCV electrodes that provides a convenient substitute to epoxy sealants.

  20. Control of Integrin αIIbβ3 Outside-In Signaling and Platelet Adhesion by Sensing the Physical Properties of Fibrin(ogen) Substrates†

    OpenAIRE

    Podolnikova, Nataly P.; Yermolenko, Ivan S.; Fuhrmann, Alexander; Lishko, Valeryi K.; Magonov, Sergei; Bowen, Benjamin; Enderlein, Joerg; Podolnikov, Andriy V.; Ros, Robert; Ugarova, Tatiana P.

    2010-01-01

    The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, ...

  1. Single-molecule force spectroscopy studies of fibrin 'A-a' polymerization interactions via the atomic force microscope

    Science.gov (United States)

    Averett, Laurel E.

    Fibrin, the polymerized form of the soluble plasma protein fibrinogen, plays a critical role in hemostasis as the structural scaffold of blood clots. The primary functions of fibrin are to withstand the shear forces of blood flow and provide mechanical stability to the clot, protecting the wound. While studies have investigated the mechanical properties of fibrin constructs, the response to force of critical polymerization interactions such as the 'A--a' knob--hole interaction remains unclear. Herein, the response of the 'A--a' bond to force was examined at the single-molecule level using the atomic force microscope. Force spectroscopy methodology was developed to examine the 'A--a' interaction while reducing the incidence of both nonspecific and multiple molecule interactions. The rupture of this interaction resulted in a previously unreported characteristic force profile comprised of up to four events. We hypothesized that the first event represented reorientation of the fibrinogen molecule, the second and third represented unfolding of structures in the D region of fibrinogen, and the last event was the rupture of the 'A--a' bond weakened by prior structural unfolding. The configuration, molecular extension, and kinetic parameters of each event in the characteristic pattern were examined to compare the unfolding of fibrin to other proteins unfolded by force. Fitting the pattern with polymer models showed that the D region of fibrinogen could lengthen by ˜50% of the length of a fibrin monomer before rupture of the 'A--a' bond. Analysis showed that the second and third events had kinetic parameters similar to other protein structures unfolded by force. Studies of the dependence of the characteristic pattern on calcium, concentration of sodium chloride, pH, and temperature demonstrated that the incidence of the last event was affected by solution conditions. However, only low pH and high temperatures reduced the probability that an interaction was characteristic

  2. In situ application of hydrogel-type fibrin-islet composite optimized for rapid glycemic control by subcutaneous xenogeneic porcine islet transplantation.

    Science.gov (United States)

    Kim, Jung-Sik; Lim, Jong-Hyung; Nam, Hye-Young; Lim, Hyun-Ju; Shin, Jun-Seop; Shin, Jin-Young; Ryu, Ju-Hee; Kim, Kwangmeyung; Kwon, Ick-Chan; Jin, Sang-Man; Kim, Hang-Rae; Kim, Sang-Joon; Park, Chung-Gyu

    2012-09-10

    Maximum engraftment of transplanted islets is essential for the clinical application of a subcutaneous site. Significant barriers to the current approaches are associated with their low effectiveness, complexity and unproven biosafety. Here, we evaluated and optimized a fibrin-islet composite for effective glycemic control in a subcutaneous site whose environment is highly hypoxic due to low vascularization potential. In the setting of xenogeneic porcine islet transplantation into the subcutaneous space of a diabetic mouse, the in vivo islet functions were greatly affected by the concentrations of fibrinogen and thrombin. The optimized hydrogel-type fibrin remarkably reduced the marginal islet mass to approximately one tenth that of islets without fibrin. This marginal islet mass was comparable to that in the setting of the subcapsular space of the kidney, which is a highly vascularized organ. Highly vascularized structures were generated inside and on the outer surface of the grafts. A hydrogel-type fibrin-islet composite established early diabetic control within an average of 3.4days after the transplantation. In the mechanistic studies, fibrin promoted local angiogenesis, enhanced islet viability and prevented fragmentation of islets into single cells. In conclusion, in situ application of hydrogel-type fibrin-islet composite may be a promising modality in the clinical success of subcutaneous islet transplantation.

  3. 指压密封胶的研制及应用%Development and application of finger press sealant

    Institute of Scientific and Technical Information of China (English)

    王永明; 王艳芳

    2013-01-01

    Finger press sealant is a soft white putty prepared with PVC resin (25%~35%),dioctyl (o-) phthalate (12%~16%),dioctyl adipate (5%~10%),tackifier (2%~5%),slip agent (8%),filler (30%~50%),etc.Its main performance is as follows:density 1.3 g/cm3,penetration (1/10mm/8s) 220,over-bake (170℃,1h):no cracks and bubbles,cold resistance (-40 ℃,3h):bend without breaking.shear strength 1.48 MPa,hardness shore A 75.People can directly manipulates it by hand when using. It is nontoxic, tasteless, not stick to hand,and can be widely used in sealing of automotive technological hole in order to reduce the noise.%指压密封胶主要由PVC树脂(25%~35%)、邻苯二甲酸二辛酯(12%~16%)、己二酸二辛酯(5%~10%)、增粘剂(2%~5%)、滑爽剂(8%)、填充剂(30%~50%)等配制而成.它的主要性能为:密度:1.3 g/cm3;针入度:220 1/10 mm/8 s;过烘烤性(170℃/1h):无龟裂和气泡产生;耐寒性(-40℃/3h):弯曲不断裂;剪切强度:1.48 MPa;邵A硬度:75.使用时可用手直接操作,无毒、无味、不粘手,广泛应用于汽车工艺孔的密封,以降低噪声.

  4. Influence of Ce, Nd, Sm and Gd oxides on the properties of alkaline-earth borosilicate glass sealant

    Directory of Open Access Journals (Sweden)

    Nibedita Sasmal

    2016-03-01

    Full Text Available In this study, the influence of CeO2, Nd2O3, Sm2O3 and Gd2O3 on various properties of the melt-quench route derived SrO–CaO–ZnO–B2O3–SiO2 glass have been investigated. Both the precursor glasses and heat treated glasses are characterized by dilatometry, differential scanning calorimetry (DSC, X-ray diffraction (XRD, field emission scanning electron microscopy (FESEM, Fourier transformed infrared spectroscopy (FTIR and X-ray photoelectron spectroscopy (XPS. The density and coefficient of thermal expansion of the glasses varies in the range 3.557–3.804 g cm−3 and 10.5–11.2 × 10−6 K−1 (50–800 °C respectively. Decrease in crystallization tendency with increase in cationic field strength of the ions is well supported by the increasing crystallization activation energy of the glasses calculated by Kissinger, Augis–Bennett and Ozawa models. XPS study revealed the presence of both Ce3+ and Ce4+ ions and an increase in characteristic binding energy of the respective rare earth elements from their core level studies. The Knoop hardness of the glasses varies in the range 6.03–6.28 GPa. The glass transition, glass softening and crystallization temperature; density and hardness of the glasses increased with increase in cationic field strength of the incorporated ions. The thermomechanical properties of the Gd2O3 containing glass advocate its applicability as the most promising sealant in solid oxide fuel cell.

  5. In search of a consensus terminology in the field of platelet concentrates for surgical use: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes.

    Science.gov (United States)

    Dohan Ehrenfest, David M; Bielecki, Tomasz; Mishra, Allan; Borzini, Piero; Inchingolo, Francesco; Sammartino, Gilberto; Rasmusson, Lars; Evert, Peter A

    2012-06-01

    In the field of platelet concentrates for surgical use, most products are termed Platelet-Rich Plasma (PRP). Unfortunately, this term is very general and incomplete, leading to many confusions in the scientific database. In this article, a panel of experts discusses this issue and proposes an accurate and simple terminology system for platelet concentrates for surgical use. Four main categories of products can be easily defined, depending on their leukocyte content and fibrin architecture: Pure Platelet-Rich Plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; Leukocyteand Platelet-Rich Plasma (L-PRP), such as Curasan, Regen, Plateltex, SmartPReP, PCCS, Magellan, Angel or GPS PRP; Pure Plaletet-Rich Fibrin (P-PRF), such as Fibrinet; and Leukocyte- and Platelet-Rich Fibrin (L-PRF), such as Choukroun's PRF. P-PRP and L-PRP refer to the unactivated liquid form of these products, their activated versions being respectively named P-PRP gels and L-PRP gels. The purpose of this search for a terminology consensus is to plead for a more serious characterization of these products. Researchers have to be aware of the complex nature of these living biomaterials, in order to avoid misunderstandings and erroneous conclusions. Understanding the biomaterials or believing in the magic of growth factors ? From this choice depends the future of the field.

  6. Fibroblast invasive migration into fibronectin/fibrin gels requires a previously uncharacterized dermatan sulfate-CD44 proteoglycan

    DEFF Research Database (Denmark)

    Clark, Richard A F; Lin, Fubao; Greiling, Doris;

    2004-01-01

    with chondroitin sulfate and dermatan sulfate, but not heparan sulfate, after a 24 h incubation with platelet-derived growth factor, the stimulus used in the migration assay. These results demonstrate that dermatan sulfate-CD44H proteoglycan is essential for fibroblast migration into fibrin clots and that platelet...... of fibronectin. Several integrins-alpha 4 beta 1, alpha 5 beta 1, and alpha v beta 3-with known fibronectin binding affinity were necessary for this invasive migration. Here we examined another family of cell surface receptors: the proteoglycans. We found that dermatan sulfate was required for fibroblast...... including heparan sulfate and chondroitin sulfate, and as such can bind fibronectin. We found that CD44H, the non-spliced isoform of CD44, was necessary for fibroblast invasion into fibronectin/fibrin gels. Resting fibroblasts expressed mostly nonglycanated CD44H core protein, which became glycanated...

  7. Multimodal CARS and SHG microscopy for label-free detection of collagen produced by hDFs in fibrin gel

    CERN Document Server

    Mortati, Leonardo; Sassi, Maria Paola

    2011-01-01

    Label-free combined CARS and SHG microscopy techniques are used as powerful tool to follow the cells behavior in cell-scaffold construct for regeneration of tissues. Imaging of histological section of hDFs seeded in fibrin gel scaffold and imaging of collagen produced by hDFs in a time course experiment at different culture days (0, 7, 21, 42) is performed. A study on the limit of collagen detection of the imaging system is reported using sample prepared with different collagen concentrations. The results show that also the small amount of collagen produced by hDFs after few hours of incubation in fibrin gel is detected. Co-localization of hDFs and collagen is also reported in function of the culture days.

  8. Severe acute respiratory failure secondary to acute fibrinous and organizing pneumonia requiring mechanical ventilation: a case report and literature review.

    Science.gov (United States)

    López-Cuenca, Sonia; Morales-García, Silvia; Martín-Hita, Ana; Frutos-Vivar, Fernando; Fernández-Segoviano, Pilar; Esteban, Andrés

    2012-08-01

    A 27-year-old woman was admitted to our ICU with acute hypoxemic respiratory failure and criteria for ARDS. Despite an F(IO(2)) of 1.0 and a lung protective strategy, the patient died on day 15 without any improvement. The relatives gave consent for post-mortem analysis. The histopathologic study of the lung showed findings typical of an acute fibrinous and organizing pneumonia. Apropos of this case we performed a PubMed search. We found 13 articles, including a total of 29 patients. Acute fibrinous and organizing pneumonia is an unusual cause of acute lung injury. The diagnostic criterion is histopathologic. There is little information regarding the pathophysiology of this illness. Important questions remain regarding this disease, including predisposing factors and management. Patients who require mechanical ventilation have poor outcomes.

  9. Lateral sliding bridge flap technique along with platelet rich fibrin and guided tissue regeneration for root coverage

    OpenAIRE

    Agarwal, Kriti; Chandra, Chetan; Agarwal, Kanika; Kumar, Nishant

    2013-01-01

    Gingival recession is defined as the apical migration of the gingival margin with exposure of root surfaces. The etiology of the condition is multifactorial. Given the high rate of gingival recession defects among the general population, it is imperative that dental practitioners have an understanding of the etiology, complications and management of the condition. A recent innovation in dentistry is the preparation and use of platelet-rich fibrin (PRF) for recession defects. The article prese...

  10. Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

    Directory of Open Access Journals (Sweden)

    Ziv-Polat O

    2012-03-01

    Full Text Available Ofra Ziv-Polat1, Hadas Skaat1, Abraham Shahar2, Shlomo Margel11Department of Chemistry, Bar-Ilan Institute of Nanotechnology and Advanced Materials, Ramat-Gan 52900, Israel; 2NVR Research Ltd, Nes-Ziona 74031, IsraelAbstract: Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGF-conjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a three-dimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin- and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.Keywords: thrombin, fibroblast growth factor, fibrin scaffold, iron oxide nanoparticles, tissue engineering, magnetism, bioactive nanoparticle

  11. Increased von Willebrand factor, P-selectin and fibrin content in occlusive thrombus resistant to lytic therapy.

    Science.gov (United States)

    Sambola, Antonia; García Del Blanco, Bruno; Ruiz-Meana, Marisol; Francisco, Jaume; Barrabés, José A; Figueras, Jaume; Bañeras, Jordi; Otaegui, Imanol; Rojas, Angeles; Vilardosa, Úrsula; Montaner, Joan; García-Dorado, David

    2016-06-01

    Therapeutic fibrinolysis is ineffective in 40 % of ST-segment elevation acute myocardial infarction (STEMI) patients, but understanding of the mechanisms is incomplete. It was our aim to compare the composition of coronary thrombus in lysis-resistant STEMI patients with that of lysis-sensitive patients. Intracoronary thrombi (n=64) were obtained by aspiration in consecutive STEMI patients. Of them, 20 had received fibrinolysis and underwent rescue percutaneous coronary intervention (r-PCI, lysis-resistant patients) and 44 underwent primary PCI (p-PCI). Lysis-sensitivity was determined in vitro by clot permeability measurements and turbidimetric lysis in plasma of 44 patients undergoing p-PCI and 20 healthy donors. Clot-lysis sensitivity was defined as a clot-lysis time not greater than 1 SD over the mean of healthy donors. Coronary thrombus composition in 20 lysis-resistant and in 20 lysis-sensitive patients was analysed by immunofluorescence with confocal microscopy. Plasma biomarkers (P-selectin, VWF, PAI-1, t-PA, D-dimer, TF pathway markers, plasmin and CD34+) were measured simultaneously on peripheral blood. Lysis-resistant clots had higher levels of fibrin (p=0.02), P-selectin (p=0.03) and VWF (p=0.01) than lysis-sensitive clots. Among thrombi obtained ≤ 6 hours after onset of symptoms, those from lysis-resistant patients showed a higher content in fibrin than those from p-PCI patients (p=0.01). Plasma PAI-1 (p=0.02) and D-dimer levels were significantly higher (p=0.003) in lysis-resistant patients, whereas plasmin levels were lower (p=0.03). Multivariate analysis showed the content of fibrin and VWF within thrombus as predictors of thrombolysis resistance. In conclusion, coronary thrombi in STEMI patients resistant to fibrinolysis are characterised by higher fibrin, P-selectin and VWF content than lysis-sensitive thrombi.

  12. A Novel Method for Iatrogenic Vesicovaginal Fistula Treatment: Autologous Platelet Rich Plasma Injection and Platelet Rich Fibrin Glue Interposition

    OpenAIRE

    2014-01-01

    Introduction: Vesicovaginal fistula remains a challenge in surgical therapy. In this study autologous platelet rich plasma and platelet rich fibrin glue were used as a minimally invasive approach for vesicovaginal fistula closure. Materials and Methods: Data including age, parity, ICIQ-UI (International Consultation on Incontinence Questionnaire-urinary incontinence), ICIQ-QOL (International Consultation on Incontinence Questionnaire-quality of life), duration of leakage, fistula d...

  13. Platelet-rich fibrin (PRF in implant dentistry in combination with new bone regenerative technique in elderly patients

    Directory of Open Access Journals (Sweden)

    Antonio Cortese, MD, DDS

    2016-01-01

    Conclusions: The main advantages in using the platelet-rich fibrin are healing and bone regenerative properties in combination with its complete resorption after surgery, thus avoiding a second surgery time, important factor in the elderly patients. Currently, it is a minimally invasive technique with low risks and satisfactory clinical results such preventing complications or implant failure particularly in elderly patients for age related conditions.

  14. A novel use of growth factors, CD34 positive cells, and fibrin for fingertip injury: Description of a case

    Directory of Open Access Journals (Sweden)

    Francesco Romano

    2016-01-01

    Full Text Available Traumatic soft tissue injuries of the finger represent a frequent hand injury challenging hand surgeons. We report a case involving a 30-year-old man with a dorsal index finger soft tissue wound failing conservative treatment. The novel use of applied fibrin membranes and concentrated growth factors yielded complete resolution of the injury in 6 months without need for skin grafting.

  15. Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium

    OpenAIRE

    Li, Jun; Zhu, Kai; Yang, Shan; WANG, YULIN; Guo, Changfa; Yin, Kanhua; Wang, Chunsheng; Lai, Hao

    2015-01-01

    Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothe...

  16. Fibrin glue repair leads to enhanced axonal elongation during early peripheral nerve regeneration in an in vivo mouse model

    Institute of Scientific and Technical Information of China (English)

    Georgios Koulaxouzidis; Gernot Reim; Christian Witzel

    2015-01-01

    Microsurgical suturing is the gold standard of nerve coaptation. Although literature on the usefulness of ifbrin glue as an alternative is becoming increasingly available, it remains contradic-tory. Furthermore, no data exist on how both repair methods might inlfuence the morphological aspects (arborization; branching) of early peripheral nerve regeneration. We used the sciatic nerve transplantation model in thy-1 yellow lfuorescent protein mice (YFP;n = 10). Pieces of nerve (1cm) were grafted from YFP-negative mice (n = 10) into those expressing YFP. We per-formed microsuture coaptations on one side and used ifbrin glue for repair on the contralateral side. Seven days after grafting, the regeneration distance, the percentage of regenerating and ar-borizing axons, the number of branches per axon, the coaptation failure rate, the gap size at the repair site and the time needed for surgical repair were all investigated. Fibrin glue repair resulted in regenerating axons travelling further into the distal nerve. It also increased the percentage of arborizing axons. No coaptation failure was detected. Gap sizes were comparable in both groups. Fibrin glue signiifcantly reduced surgical repair time. The increase in regeneration distance, even after the short period of time, is in line with the results of others that showed faster axonal regen-eration after ifbrin glue repair. The increase in arborizing axons could be another explanation for better functional and electrophysiological results after ifbrin glue repair. Fibrin glue nerve coap-tation seems to be a promising alternative to microsuture repair.

  17. Fibrin adhesive implant in wound healing repair of dental sockets with topical application of epsilon aminocaproic acid: histological analysis.

    Science.gov (United States)

    Padovan, Luis Eduardo M; Okamoto, Tetuo; Rezende, Maria Cristina Rossifini Alves; Curvêllo, Victor Prado; Nicolielo, Daniela; Matsumoto, Mariza Akemi

    2005-05-01

    The aim of the study was to evaluate wound healing repair of dental sockets after topical application of 5% epsilon-aminocaproic acid (EACA) and the use of fibrin adhesive implant in rats under anticoagulant therapy with warfarin. Sixty Albinus wistar rats were used, divided into three groups of 20. In Group I, the animals were given 0.1 mL/100 mg of 0.9% saline solution per day, beginning 6 days before dental extraction and continuing throughout the experimental period. In Group II, the animals received 0.03 mL of sodium warfarin daily, beginning 6 days before the surgery and continuing until the day of sacrifice; after tooth extractions, the sockets were filled with fibrin adhesive material. In Group III the animals were treated as in Group II, and after extractions, the sockets were irrigated with 5 mL of 5% EACA and filled with the same fibrin adhesive material. All groups presented biological phases of wound healing repair, the differences being evident only in the chronology. The results obtained in Group III were very similar to those of Group I in the last period of wound repair, whereas Group II presented a late chronology compared to the other groups.

  18. Radioimmunoassay of fragment E-related neoantigen: validation studies and clinical application. [Fibrinogen-fibrin degradation products

    Energy Technology Data Exchange (ETDEWEB)

    Chen, J.P.; Hanna, W.T.; Williams, T.K.; Krauss, S. (Tennessee Univ., Knoxville (USA). Memorial Research Center and Hospital)

    1984-05-01

    An E-neoantigen radioimmunoassay (Eneo RIA) is described which can determine normal and pathological plasma levels of E-related fibrinogen-fibrin degradation products (FDP). The assay employs rabbit antiserum produced against fragment E derived from a plasmin digest of fibrinogen and subsequently absorbed with fibrinogen. The absorbed antiserum contains antibodies which are equally reactive with fibrinogen derived E (Fg-E) and fibrin derived E(Fb-E) but not with fibrinogen at 1 mg/ml. The Eneo RIA was validated by assay parallelism and by recovery experiments. Plasma Eneo immunoreactivities in 14 normals were 4-22 ng/ml (mean 12.7 ng/ml). Plasma Eneo levels in 23 of 24 patients with neoplastic and haematological diseases were elevated above normal (range 27-2027 ng/ml). Unusually high Eneo values were observed with three patients whose diseases were complicated by either disseminated intravascular coagulation (DIC) or deep vein thrombosis. After heparin therapy, the Eneo level of a patient with chronic DIC declined. A pathological plasma was eluted from a Sephadex G-200 column and Eneo immunoreactivity was determined on the eluates. The gel filtration pattern of Eneo indicates that E-related FDP is a family of plasmic fragments derived from crosslinked fibrin.

  19. The Effect of Chondroitin Sulphate and Hyaluronic Acid on Chondrocytes Cultured within a Fibrin-Alginate Hydrogel

    Directory of Open Access Journals (Sweden)

    Christopher J. Little

    2014-09-01

    Full Text Available Osteoarthritis is a painful degenerative joint disease that could be better managed if tissue engineers can develop methods to create long-term engineered articular cartilage tissue substitutes. Many of the tissue engineered cartilage constructs currently available lack the chemical stimuli and cell-friendly environment that promote the matrix accumulation and cell proliferation needed for use in joint cartilage repair. The goal of this research was to test the efficacy of using a fibrin-alginate hydrogel containing hyaluronic acid (HA and/or chondroitin sulphate (CS supplements for chondrocyte culture. Neonatal porcine chondrocytes cultured in fibrin-alginate hydrogels retained their phenotype better than chondrocytes cultured in monolayer, as evidenced by analysis of their relative expression of type II versus type I collagen mRNA transcripts. HA or CS supplementation of the hydrogels increased matrix glycosaminoglycan (GAG production during the first week of culture. However, the effects of these supplements on matrix accumulation were not additive and were no longer observed after two weeks of culture. Supplementation of the hydrogels with CS or a combination of both CS and HA increased the chondrocyte cell population after two weeks of culture. Statistical analysis indicated that the HA and CS treatment effects on chondrocyte numbers may be additive. This research suggests that supplementation with CS and/or HA has positive effects on cartilage matrix production and chondrocyte proliferation in three-dimensional (3D fibrin-alginate hydrogels.

  20. Vampire bat salivary plasminogen activator is quiescent in human plasma in the absence of fibrin unlike human tissue plasminogen activator.

    Science.gov (United States)

    Gardell, S J; Hare, T R; Bergum, P W; Cuca, G C; O'Neill-Palladino, L; Zavodny, S M

    1990-12-15

    The vampire bat salivary plasminogen activator (Bat-PA) is a potent PA that exhibits remarkable selectivity toward fibrin-bound plasminogen (Gardell et al, J Biol Chem 256: 3568, 1989). Herein, we describe the activity of recombinant DNA-derived Bat-PA (rBat-PA) in a human plasma milieu. rBat-PA and recombinant human single-chain tissue plasminogen activator (rt-PA) are similarly efficacious at lysing plasma clots. In stark contrast to rt-PA, the addition of 250 nmol/L rBat-PA to plasma in the absence of a clot failed to deplete plasminogen, alpha 2-antiplasmin and fibrinogen. The lytic activities exhibited by finger-domain minus Bat-PA (F- rBat-PA) and finger and epidermal growth factor-like domains minus Bat-PA (FG- rBat-PA) were less than rBat-PA, especially at low concentrations of PA; nevertheless, these truncated forms also possessed a strict requirement for a fibrin cofactor. The loss of PA activity following the addition of rBat-PA to plasma was slower than that observed when either rt-PA or two-chain rt-PA was added. The efficacy, fibrin selectivity, and decreased susceptibility to inactivation exhibited by rBat-PA in vitro in a human plasma milieu suggests that rBat-PA may be superior to rt-PA for the treatment of thrombotic complications.

  1. Full regeneration of segmental bone defects using porous titanium implants loaded with BMP-2 containing fibrin gels

    Directory of Open Access Journals (Sweden)

    J van der Stok

    2015-03-01

    Full Text Available Regeneration of load-bearing segmental bone defects is a major challenge in trauma and orthopaedic surgery. The ideal bone graft substitute is a biomaterial that provides immediate mechanical stability, while stimulating bone regeneration to completely bridge defects over a short period. Therefore, selective laser melted porous titanium, designed and fine-tuned to tolerate full load-bearing, was filled with a physiologically concentrated fibrin gel loaded with bone morphogenetic protein-2 (BMP-2. This biomaterial was used to graft critical-sized segmental femoral bone defects in rats. As a control, porous titanium implants were either left empty or filled with a fibrin gels without BMP-2. We evaluated bone regeneration, bone quality and mechanical strength of grafted femora using in vivo and ex vivo µCT scanning, histology, and torsion testing. This biomaterial completely regenerated and bridged the critical-sized bone defects within eight weeks. After twelve weeks, femora were anatomically re-shaped and revealed open medullary cavities. More importantly, new bone was formed throughout the entire porous titanium implants and grafted femora regained more than their innate mechanical stability: torsional strength exceeded twice their original strength. In conclusion, combining porous titanium implants with a physiologically concentrated fibrin gels loaded with BMP-2 improved bone regeneration in load-bearing segmental defects. This material combination now awaits its evaluation in larger animal models to show its suitability for grafting load-bearing defects in trauma and orthopaedic surgery.

  2. Microbicidal properties of Leukocyte- and Platelet-Rich Plasma/Fibrin (L-PRP/L-PRF): new perspectives.

    Science.gov (United States)

    Cieslik-Bielecka, A; Dohan Ehrenfest, D M; Lubkowska, A; Bielecki, T

    2012-01-01

    Platelets, as main actors of the first stage of the healing process, play an important role in tissue repair. Their granules contain many active substances, particularly over 30 growth factors with significant effects on the resident cells at the site of injury, such as mesenchymal stem cells, chondrocytes, fibroblasts, osteoblasts. This potential may be increased by the concentration of the platelets, using platelet-rich plasma/fibrin products. In the four families of platelet concentrates, 2 families contain also significant concentrations of leukocytes: L-PRP (Leukocyte- and Platelet-Rich Plasma) and L-PRF (Leukocyte- and Platelet-Rich Fibrin). Inductive properties of platelet concentrates were widely described. However, they present also antimicrobial effects. The antibacterial effects of L-PRP were highlighted in only a few in vitro studies. Strong activity comparable to gentamicin and oxacillin for L-PRP against methicillin susceptible Staphylococcus aureus (MSSA) was already demonstrated. L-PRP also inhibited the growth of methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli. Some authors also reported clinical observations about the reduction of infections and the induction of healing processes after the use of platelet concentrates in cardiac, orthopaedic, oral and maxillofacial surgery. However, very little is yet known about the antibacterial effects of these concentrates. In this manuscript, the current data about the antimicrobial agents and cells present in the platelet-rich plasma/fibrin are highlighted and discussed, in order to introduce this new key chapter of the platelet concentrate technology history.

  3. In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate.

    Science.gov (United States)

    Tunalı, Mustafa; Özdemir, Hakan; Küçükodacı, Zafer; Akman, Serhan; Fıratlı, Erhan

    2013-07-01

    We have developed a new, titanium-prepared, platelet-rich fibrin (T-PRF) together with the protocol for forming it, which is based on the hypothesis that titanium tubes may be more effective at activating platelets than the glass tubes used by Chouckroun in his platelet-rich fibrin (PRF) method. The aim of this study was to find a suitable animal model in which to evaluate the method and to investigate the efficacy of T-PRF for wound healing. Blood samples from 6 rabbits were used to confirm the protocol for formation of T-PRF. We evaluated T-PRF or T-PRF-like clots morphologically using scanning electron microscopy (EM). Blood samples from 5 rabbits were used to develop an experiment in which to evaluate the effects of T-PRF on wound healing. The mucoperiosteal flaps were filled with autologous T-PRF membranes from the vestibule in the anterior mandibular regions. Samples collected from the surgical sites were stained with haematoxylin and eosin. We found a mature fibrin network in T-PRF clots that had been centrifuged for 15 min at 3500 rpm and, 15 days after placement of the membrane, we found newly-forming connective tissue and islets of bony tissue in the T-PRF membrane. These results show that T-PRF could induce the formation of new bone with new connective tissue in a rabbit model of wound healing within 30 days of treatment.

  4. Tracheal reconstruction using chondrocytes seeded on a poly(L-lactic-co-glycolic acid)-fibrin/hyaluronan.

    Science.gov (United States)

    Hong, Hyun Jun; Chang, Jae Won; Park, Ju-Kyeong; Choi, Jae Won; Kim, Yoo Suk; Shin, Yoo Seob; Kim, Chul-Ho; Choi, Eun Chang

    2014-11-01

    Reconstruction of trachea is still a clinical dilemma. Tissue engineering is a recent and promising concept to resolve this problem. This study evaluated the feasibility of allogeneic chondrocytes cultured with fibrin/hyaluronic acid (HA) hydrogel and degradable porous poly(L-lactic-co-glycolic acid) (PLGA) scaffold for partial tracheal reconstruction. Chondrocytes from rabbit articular cartilage were expanded and cultured with fibrin/HA hydrogel and injected into a 5 × 10 mm-sized, curved patch-shape PLGA scaffold. After 4 weeks in vitro culture, the scaffold was implanted on a tracheal defect in eight rabbits. Six and 10 weeks postoperatively, the implanted sites were evaluated by bronchoscope and radiologic and histologic analyses. Ciliary beat frequency (CBF) of regenerated epithelium was also evaluated. None of the eight rabbits showed any sign of respiratory distress. Bronchoscopic examination did not reveal stenosis of the reconstructed trachea and the defects were completely recovered with respiratory epithelium. Computed tomography scan showed good luminal contour of trachea. Histologic data showed that the implanted chondrocytes successfully formed neocartilage with minimal granulation tissue. CBF of regenerated epithelium was similar to that of normal epithelium. Partial tracheal defect was successfully reconstructed anatomically and functionally using allogeneic chondrocytes cultured with PLGA-fibrin/HA composite scaffold.

  5. Morphogen and proinflammatory cytokine release kinetics from PRGF-Endoret fibrin scaffolds: evaluation of the effect of leukocyte inclusion.

    Science.gov (United States)

    Anitua, E; Zalduendo, M M; Prado, R; Alkhraisat, M H; Orive, G

    2015-03-01

    The potential influence of leukocyte incorporation in the kinetic release of growth factors from platelet-rich plasma (PRP) may explain the conflicting efficiency of leukocyte platelet-rich plasma (L-PRP) scaffolds in tissue regeneration. To assess this hypothesis, leukocyte-free (PRGF-Endoret) and L-PRP fibrin scaffolds were prepared, and both morphogen and proinflammatory cytokine release kinetics were analyzed. Clots were incubated with culture medium to monitor protein release over 8 days. Furthermore, the different fibrin scaffolds were morphologically characterized. Results show that leukocyte-free fibrin matrices were homogenous while leukocyte-containing ones were heterogeneous, loose and cellular. Leukocyte incorporation produced a significant increase in the contents of proinflammatory cytokines interleukin (IL)-1β and IL-16 but not in the platelet-derived growth factors release (<1.5-fold). Surprisingly, the availability of vascular endothelial growth factor suffered an important decrease after 3 days of incubation in the case of L-PRP matrices. While the release of proinflammatory cytokines was almost absent or very low from PRGF-Endoret, the inclusion of leukocytes induced a major increase in these cytokines, which was characterized by the presence of a latent period. The PRGF-Endoret matrices were stable during the 8 days of incubation. The inclusion of leukocytes alters the growth factors release profile and also increased the dose of proinflammatory cytokines.

  6. High-throughput proteomic characterization of plasma rich in growth factors (PRGF-Endoret)-derived fibrin clot interactome.

    Science.gov (United States)

    Anitua, Eduardo; Prado, Roberto; Azkargorta, Mikel; Rodriguez-Suárez, Eva; Iloro, Ibon; Casado-Vela, Juan; Elortza, Felix; Orive, Gorka

    2015-11-01

    Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this technology is a clot mainly composed of fibrin protein, forming a three-dimensional (3D) macroscopic network. This biomaterial is easily obtained by biotechnological means from blood and can be used in a range of situations to help wound healing and tissue regeneration. Although the main constituent of this clot is the fibrin scaffold, little is known about other proteins interacting in this clot that may act as adjuvants in the healing process. The aim of this study was to characterize the proteins enclosed by PRGF-Endoret scaffold, using a double-proteomic approach that combines 1D-SDS-PAGE approach followed by LC-MS/MS, and 2-DE followed by MALDI-TOF/TOF. The results presented here provide a description of the catalogue of key proteins in close contact with the fibrin scaffold. The obtained lists of proteins were grouped into families and networks according to gene ontology. Taken together, an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing has been found.

  7. ASTIGMATIC OUTCOME & POST OPERATIVE RECOVERY IN PAT IENTS UNDERGOING PTERYGIUM EXCISION WITH CONJUNCTIVAL AUTOGRAFTS. COMPARISION BETWEEN SUTURE & AUTOLOGUS BLOOD FIBRIN

    Directory of Open Access Journals (Sweden)

    Smita Dileep

    2013-06-01

    Full Text Available ABSTRACT : AIMS: To review the effect of surgery type on the postop erative recovery & astigmatism in pterygium surgery. SETTINGS AND DESIGN: Randomized comparative clinical trial. MATERIALS AND METHODS: Forty six patients (46 eyes with primary pterygiu m were randomised to undergo pterygium surgery using eithe r autologous fibrin glue (23 eyes or 10-0 nylon sutures (23 eyes to attach the conjunctival autograft. The patients were followed up 1day, 1wk, 2wks & 6 w eeks. Outcome measures were keratometry changes & postope rative recovery.. The preoperative and postoperative keratometric measurements, evaluated using keratometer, were noted. RESULTS: Corneal astigmatism was significantly reduced in th e total group from 2.94(SD- 0.52 diopter to 1.91 (sd 0.57 diopter (p 0.05. The intensity of the postoperative watering, itchin g, were significantly lower in the autologous fibrin glue group than in the suture gro up (p<0.001. Postoperative redness was absent in 47.82% (11cases and mild in 52.18% (13 cases 2 weeks postoperatively in the autologous fibrin group while in sutured group 17.4% (4cases had mild redness and 82.6% (19 cases had moderate redness 2weeks postoperatively.

  8. Quercetin impregnated chitosan-fibrin composite scaffolds as potential wound dressing materials - Fabrication, characterization and in vivo analysis.

    Science.gov (United States)

    Vedakumari, Weslen S; Ayaz, Nazeeha; Karthick, Arun S; Senthil, Rethinam; Sastry, Thotapalli P

    2017-01-15

    The present study efforts at fabricating chitosan-fibrin composite (CF) scaffolds impregnated with quercetin for wound dressing application and aims at investigating their physicochemical properties. CF scaffolds were prepared by mixing acidic solution of chitosan with an alkaline solution of fibrin, to which quercetin (Q) was added, homogenized and lyophilized obtain Q-CF scaffold. FTIR spectra were used to determine the interactions between the functional groups of quercetin and CF scaffolds. TGA analysis revealed the decomposition of saccharide rings and amino acids of chitosan and fibrin at the temperature range of 255-400°C. Q-CF scaffold exhibited maximum tensile strength of 1.45MPa, an ideal mechanical strength for a wound dressing material. Q-CF scaffolds exhibited good bactericidal activity against Escherichia coli and Staphylococcus aureus. Biocompatibility of Q-CF scaffold was assessed using MTT assay, which elucidated its non-toxic property and excellent suitability for tissue engineering applications. In vivo wound healing experiments performed using albino rats revealed that topical application of Q-CF scaffold on open excision type of wounds can significantly accelerate the process of wound healing. These results suggest that Q-CF scaffold could serve as a promising wound dressing material.

  9. Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

    Directory of Open Access Journals (Sweden)

    Chiara Gardin

    2011-10-01

    Full Text Available Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.

  10. 有机硅密封胶在车灯密封上的应用优势%Application Advantages of Silicone Sealant on Automobile Lights

    Institute of Scientific and Technical Information of China (English)

    张斌; 谢林; 屈裴

    2015-01-01

    This article analyzes the application advantages of silicone sealant on automobile lights in adhe-sive strength , high temperature resistance , flexibility under low temperature and ultraviolet resistance .Gener-ally, after 14 days ’ maintenance, home -made silicone sealant for automobile lights keeps an adhesive strength of 1.25 MPa, and it has no variation even after 6 hours torrefaction at 150℃.No cracks, lamination, and adhesive damages are found during low temperature flexibility test .There is little variation of tensile strength and elongation at break after 320 nm under ultraviolet radiation .%从粘接强度、耐高温性能、低温柔性、耐紫外光等几方面论述了有机硅密封胶在车灯密封上的应用优势。自制的车灯用有机硅密封胶养护14天后粘接强度为1.25 MPa;在150℃下烘烤6 h后,力学性能没有变化;低温柔性测试后没有出现开裂、部分分层、粘接损坏等现象;经320 nm紫外线照射后,密封胶的拉伸强度及拉断伸长率变化很小。

  11. Influence of irradiation by a novel CO2 9.3-μm short-pulsed laser on sealant bond strength.

    Science.gov (United States)

    Rechmann, P; Sherathiya, K; Kinsel, R; Vaderhobli, R; Rechmann, B M T

    2017-04-01

    The objective of this in vitro study was to evaluate whether irradiation of enamel with a novel CO2 9.3-μm short-pulsed laser using energies that enhance caries resistance influences the shear bond strength of composite resin sealants to the irradiated enamel. Seventy bovine and 240 human enamel samples were irradiated with a 9.3-μm carbon dioxide laser (Solea, Convergent Dental, Inc., Natick, MA) with four different laser energies known to enhance caries resistance or ablate enamel (pulse duration from 3 μs at 1.6 mJ/pulse to 43 μs at 14.9 mJ/pulse with fluences between 3.3 and 30.4 J/cm(2), pulse repetition rate between 4.1 and 41.3 Hz, beam diameter of 0.25 mm and 1-mm spiral pattern, and focus distance of 4-15 mm). Irradiation was performed "freehand" or using a computerized, motor-driven stage. Enamel etching was achieved with 37% phosphoric acid (Scotchbond Universal etchant, 3M ESPE, St. Paul, MN). As bonding agent, Adper Single Bond Plus was used followed by placing Z250 Filtek Supreme flowable composite resin (both 3M ESPE). After 24 h water storage, a single-plane shear bond test was performed (UltraTester, Ultradent Products, Inc., South Jordan, UT). All laser-irradiated samples showed equal or higher bond strength than non-laser-treated controls. The highest shear bond strength values were observed with the 3-μs pulse duration/0.25-mm laser pattern (mean ± SD = 31.90 ± 2.50 MPa), representing a significant 27.4% bond strength increase over the controls (25.04 ± 2.80 MPa, P ≤ 0.0001). Two other caries-preventive irradiation (3 μs/1 mm and 7 μs/0.25 mm) and one ablative pattern (23 μs/0.25 mm) achieved significantly increased bond strength compared to the controls. Bovine enamel also showed in all test groups increased shear bond strength over the controls. Computerized motor-driven stage irradiation did not show superior bond strength values over the clinically more relevant freehand irradiation. Enamel

  12. 华硅6CF阻燃耐候密封胶的研发及应用%Development and application of Huagui 6CF fire retardant and weather resisting sealant

    Institute of Scientific and Technical Information of China (English)

    郭传发; 刘扬秀

    2016-01-01

    用自制铂-硼-硅阻燃剂与107硅橡胶、201硅油等反应制得了6CF阻燃硅酮耐候密封胶,并对其性能进行了研究。结果表明,当加入10份阻燃剂时,6CF阻燃耐候硅酮密封胶既具有FV-0级的阻燃性能又不会对物理力学性能、贮存期产生影响。并将6CF阻燃耐候硅酮密封胶成功应用于上海中心工程中。%The 6CF fire retardant and weather resisting sealant was prepared by reacting of the home-made boron-platinum-silicon fire retardant, 107 silicone rubber and 201 silicone oil, etc. The performance of the sealant was studied. The results showed that when the content of the fire retardant was 10 phrs, the 6CF fire retardant sealant not only gave a FV-0 grade flame retardancy, but also did not produce adverse effects on its mechanical properties and storage period. The 6CF fire retardant sealant was successfully used in the engineering of Shanghai Tower.

  13. Replacing epoxy adhesive with photosensitive anaerobic sealant for gyroscope%光敏厌氧密封胶替代环氧胶在陀螺仪中的应用

    Institute of Scientific and Technical Information of China (English)

    杨丹丹; 杨强强; 李刚

    2013-01-01

    介绍了1种用于陀螺仪的新型光敏厌氧密封胶,对其进行了密封性、高低温循环、耐油性等测试,结果表明,该胶能够满足液浮陀螺仪密封的指标要求,可替代传统的环氧陀螺仪密封胶,并已首先应用于陀螺仪关键部位引线柱的密封.%A novel photosensitive anaerobic sealant for gyroscope was introduced. The tightness, resistance of high and low temperature cycle and oil resistance of the sealant were tested. The results showed that the sealant could meet the requirements of the liquid floating gyroscope sealing indicators and alternative the conventional epoxy gyroscope sealant. It was first applied in sealing of lead column which was the key parts of gyroscope.

  14. Introduction to application of spot welding sealants in manufacture of white body%浅谈点焊密封胶在白车身制造中的应用

    Institute of Scientific and Technical Information of China (English)

    王晓宁

    2013-01-01

    The spot welding sealant should be applied within the welding joints to ensure the leak and air tightness of the finished automobiles and meet the increasingly stringent requirements of NVH and rust prevention.The classification,using process and performance of the spot welding sealants were introduced.The actual production experience showed that the selection of suitable spot welding sealants,curing process and sealant spreading has an important role to assure the quality and performance of the finished aotomobiles.%为保证整车的密封性,满足日益严格的NVH与防锈要求,需在焊缝间涂布点焊密封胶。介绍了点焊密封胶分类、使用工艺及性能。实际生产经验表明,选择合适的点焊密封胶、固化工艺及涂胶量,对保证整车品质和性能具有重要作用。

  15. Optical Measurement of Micromechanics and Structure in a 3D Fibrin Extracellular Matrix

    Science.gov (United States)

    Kotlarchyk, Maxwell Aaron

    2011-07-01

    In recent years, a significant number of studies have focused on linking substrate mechanics to cell function using standard methodologies to characterize the bulk properties of the hydrogel substrates. However, current understanding of the correlations between the microstructural mechanical properties of hydrogels and cell function in 3D is poor, in part because of a lack of appropriate techniques. Methods for tuning extracellular matrix (ECM) mechanics in 3D cell culture that rely on increasing the concentration of either protein or cross-linking molecules fail to control important parameters such as pore size, ligand density, and molecular diffusivity. Alternatively, ECM stiffness can be modulated independently from protein concentration by mechanically loading the ECM. We have developed an optical tweezers-based microrheology system to investigate the fundamental role of ECM mechanical properties in determining cellular behavior. Further, this thesis outlines the development of a novel device for generating stiffness gradients in naturally derived ECMs, where stiffness is tuned by inducing strain, while local structure and mechanical properties are directly determined by laser tweezers-based passive and active microrheology respectively. Hydrogel substrates polymerized within 35 mm diameter Petri dishes are strained non-uniformly by the precise rotation of an embedded cylindrical post, and exhibit a position-dependent stiffness with little to no modulation of local mesh geometry. Here we present microrheological studies in the context of fibrin hydrogels. Microrheology and confocal imaging were used to directly measure local changes in micromechanics and structure respectively in unstrained hydrogels of increasing fibrinogen concentration, as well as in our strain gradient device, in which the concentration of fibrinogen is held constant. Orbital particle tracking, and raster image correlation analysis are used to quantify changes in fibrin mechanics on the

  16. The use of platelet-rich fibrin membrane in gingival recession treatment

    Directory of Open Access Journals (Sweden)

    Aleksić Zoran

    2010-01-01

    Full Text Available Introduction. Fibrin, fibronectin, platelet derived growth factor, and transforming growth factors from platelet concetrate are crucial for tissue reparation and regeneration. Objective. This study was designed to evaluate clinical effectiveness of activated platelet-rich fibrin (PRF membrane in treatment of gingival recession. Methods. 19 gingival recessions Miller class I or II were treated with a coronally advanced flap and the PRF membrane (PRF group. Following the elevation of the flap, bone and root surfaces were covered with the PRF membrane. After suturing, the PRF membrane was covered with a coronally advanced flap. In the same patients, 19 other gingival recessions were treated with CTG in combination with the coronally advanced flap (the CTG group. Clinical recordings were made of vertical recession depth (VRD, probing depth (PD, clinical attachment level (CAL and keratinized tissue width (KTW before and 12 months after mucogingival surgical treatment. Clinical evaluation of healing events was estimated with recordings of the healing index (HI. Recordings of HI were performed in the 1st, 2nd and 3rd week post-surgically. Results. Mean root coverage was significant in both groups (the PRF group 79.94% and the CTG group 88.56% %; p<0.01. The difference between the two tested groups was not statistically significant. Results of the keratinized tissue width showed significant increase (p<0.05 12 months after the surgery in both, the PRF and CTG groups. Results of KTW showed statistical significance of recorded differences obtained in the two evaluated groups (p<0.05. There was no statistical significance in reduction of PD and CAL recorded in the PRF and CTG groups. The values of HI recorded in the 1st and 2nd week postoperatively were significantly enhanced in the PRF group (p<0.05. Conclusion. Results of this study confirm both procedures as effective with equivalence of clinical results in solving gingival recession problems. The

  17. Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling.

    Science.gov (United States)

    Bento, Ana R; Quelhas, Pedro; Oliveira, Maria J; Pêgo, Ana P; Amaral, Isabel F

    2016-12-29

    In an attempt to improve the efficacy of neural stem/progenitor cell (NSPC) based therapies, fibrin hydrogels are being explored to provide a favourable microenvironment for cell survival and differentiation following transplantation. In the present work, the ability of fibrin to support the survival, proliferation, and neuronal differentiation of NSPCs derived from embryonic stem (ES) cells under monolayer culture was explored. Single mouse ES-NSPCs were cultured within fibrin (fibrinogen concentration: 6 mg/ml) under neuronal differentiation conditions up to 14 days. The ES-NSPCs retained high cell viability and proliferated within small-sized spheroids. Neuronal differentiation was confirmed by an increase in the levels of βIII-tubulin and NF200 over time. At day 14, cell-matrix constructs mainly comprised NSPCs and neurons (46.5% βIII-tubulin(+) cells). Gamma-aminobutyric acid (GABA)ergic and dopaminergic/noradrenergic neurons were also observed, along with a network of synaptic proteins. The ES-NSPCs expressed matriptase and secreted MMP-2/9, with MMP-2 activity increasing along time. Fibronectin, laminin and collagen type IV deposition was also detected. Fibrin gels prepared with higher fibrinogen concentrations (8/10 mg/ml) were less permissive to neurite extension and neuronal differentiation, possibly owing to their smaller pore area and higher rigidity. Overall, it is shown that ES-NSPCs within fibrin are able to establish neuronal networks and to remodel fibrin through MMP secretion and extracellular matrix (ECM) deposition. This three-dimensional (3D) culture system was also shown to support cell viability, neuronal differentiation and ECM deposition of human ES-NSPCs. The settled 3D platform is expected to constitute a valuable tool to develop fibrin-based hydrogels for ES-NSPC delivery into the injured central nervous system. Copyright © 2016 John Wiley & Sons, Ltd.

  18. Diagnostic and genetic studies on fibrin-stabilizing factor with a new assay based on amine incorporation.

    Science.gov (United States)

    Lorand, L; Urayama, T; De Kiewiet, J W; Nossel, H L

    1969-06-01

    Fibrinoligase, the fibrin cross-linking enzyme, transiently appearing during the course of coagulation in normal blood, was shown to catalyze the incorporation of a fluorescent amine, monodansylcadaverine [or N-(5-aminopentyl)-5-dimethylamino-1-naphthalene-sulfonamide] into casein. The reaction provided the basis of a sensitive fluorimetric method for measuring the activity of the enzyme (and also of similar other transpeptidases, such as transglutaminase). In tests involving plasma, certain difficulties had to be overcome which were mainly due to the fact that the enzyme itself does not occur in citrated plasma. Only its precursor (fibrin-stabilizing factor or factor XIII) is present, still requiring limited proteolytic activation by thrombin. Thus, in order to measure amine incorporation with plasma as a source of the factor, thrombin must be added. This necessitated a differential desensitization of the intrinsic fibrinogen so that the latter could not clot and could not thereby interfere with amine incorporation. Also, the thrombin-inactivating capacity of plasma had to be saturated to enable full conversion of the factor to the transpeptidase. Concentrations of casein, monodansylcadaverine, calcium, and hydrogen ions were chosen to permit almost maximal velocity of amine incorporation. A linear relationship with regard to plasma concentration could be obtained only under such conditions. No similar assay is presently available for quantitatively evaluating fibrin-stabilizing factor levels in plasma.The amine incorporation test was applied to a clinical case of hereditary total fibrin-stabilizing factor deficiency. The effect of transfusion therapy was studied, and some of the patient's relatives were examined. Whereas a paternal aunt and uncle gave values well within the normal range, a brother and the mother proved to be partially deficient and could be considered as heterozygous carriers. The father appeared to have a reduced level of fibrin

  19. A new device for measurement of fibrin clot lysis: application to the Euglobulin Clot Lysis Time

    Directory of Open Access Journals (Sweden)

    Brohée D

    2002-05-01

    Full Text Available Abstract Background Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely computerised semi-automatic 8-channel device for measurement and determination of fibrin clot lysis. The lysis time is evaluated by a mathematical analysis of the lysis curve and the results are expressed in minute (range: 5 to 9999. We have used this new device for Euglobulin Clot Lysis Time (ECLT determination, which is the most common test used in laboratories to estimate plasma fibrinolytic capacity. Results The correlation between ECLT and manual method is very tight : R = 0,99; p -6. The efficiency scores of the method are Conclusions The routine use of this new device could be useful in various situations such as assessment in atherosclerosis and arteriosclerosis associated diseases, coagulation survey of liver transplantations, cardiovascular surgery or pharmacological research. It has already provided highly promising results in preliminary studies on the relation between fibrinolysis and cardiovascular risk factors.

  20. A new device for measurement of fibrin clot lysis: application to the Euglobulin Clot Lysis Time

    Science.gov (United States)

    Boudjeltia, K Zouaoui; Cauchie, Ph; Remacle, Cl; Guillaume, M; Brohée, D; Hubert, JL; Vanhaeverbeek, M

    2002-01-01

    Background Determination of clot lysis times on whole blood, diluted whole blood, plasma or plasma fraction has been used for many years to assess the overall activity of the fibrinolytic system. We designed a completely computerised semi-automatic 8-channel device for measurement and determination of fibrin clot lysis. The lysis time is evaluated by a mathematical analysis of the lysis curve and the results are expressed in minute (range: 5 to 9999). We have used this new device for Euglobulin Clot Lysis Time (ECLT) determination, which is the most common test used in laboratories to estimate plasma fibrinolytic capacity. Results The correlation between ECLT and manual method is very tight : R = 0,99; p < 10-6. The efficiency scores of the method are <4% in intra-assay and <7% in inter-assay. It allows to achieve the tests on hyperlipaemic samples. This new device has been easily integrated in laboratory routine and allows to achieve several ECLT every day without disturbance of laboratory workflow. Conclusions The routine use of this new device could be useful in various situations such as assessment in atherosclerosis and arteriosclerosis associated diseases, coagulation survey of liver transplantations, cardiovascular surgery or pharmacological research. It has already provided highly promising results in preliminary studies on the relation between fibrinolysis and cardiovascular risk factors. PMID:11985782