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Sample records for artichoke est database

  1. An EST database from saffron stigmas

    Directory of Open Access Journals (Sweden)

    Chiusano Maria Luisa

    2007-10-01

    Full Text Available Abstract Background Saffron (Crocus sativus L., Iridaceae flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part – the stigmas – are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. Results We produced 6,603 high quality Expressed Sequence Tags (ESTs from a saffron stigma cDNA library. This collection is accessible and searchable through the Saffron Genes database http://www.saffrongenes.org. The ESTs have been grouped into 1,893 Clusters, each corresponding to a different expressed gene, and annotated. The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database, allowing users to investigate sequence qualities and EST structural features (vector contamination, repeat regions. The saffron stigma transcriptome contains a series of interesting sequences (putative sex determination genes, lipid and carotenoid metabolism enzymes, transcription factors. Conclusion The Saffron Genes database represents the first reference collection for the genomics of Iridaceae, for the molecular biology of stigma biogenesis, as well as for the metabolic pathways underlying saffron secondary metabolism.

  2. Signaling pathways in a Citrus EST database

    Directory of Open Access Journals (Sweden)

    Angela Mehta

    2007-01-01

    Full Text Available Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis was launched in order to sequence Citrus ESTs (expressed sequence tags from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile. Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

  3. Update History of This Database - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AcEST Update... History of This Database Date Update contents 2013/01/10 Errors found on AcEST Conting data hav...iew/acest/ ) is released. Joomla SEF URLs by Artio About This Database Database Description Download License Update... History of This Database Site Policy | Contact Us Update History of This Database - AcEST | LSDB Archive ...

  4. Update History of This Database - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ClEST Update... History of This Database Date Update contents 2012/05/22 ClEST English archive site is opened. ...Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Da...tabase Site Policy | Contact Us Update History of This Database - ClEST | LSDB Archive ...

  5. CyMSatDB: The Globe Artichoke (Cynara cardunculus var. scolymus) Microsatellite Database

    DEFF Research Database (Denmark)

    Portis, Ezio; Portis, Flavio; Valente, Luisa;

    2015-01-01

    in a MySQL database and provides an effective and responsive interface developed in PHP. To cater the customized needs of wet lab, features with a novelty of an automated primer designing tool is added. The feature of user defined primer designing has great advantage in terms of precise selection from...

  6. CyMSatDB: The Globe Artichoke (Cynara cardunculus var. scolymus) Microsatellite Database

    DEFF Research Database (Denmark)

    Portis, Ezio; Portis, Flavio; Valente, Luisa;

    in a MySQL database and provides an effective and responsive interface developed in PHP. To cater the customized needs of wet lab, features with a novelty of an automated primer designing tool is added. The feature of user defined primer designing has great advantage in terms of precise selection from...

  7. AcEST Contig - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...contig.zip File URL: ftp://ftp.biosciencedbc.jp/archive/acest/LATEST/acestcontig....zip File size: 1.34MB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acestcontig#en Da...Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us AcEST Contig - AcEST | LSDB Archive ...

  8. Signaling pathways in a Citrus EST database

    OpenAIRE

    Angela Mehta; Marilia Santos Silva; Simone Guidetti-Gonzalez; Helaine Carrer; Marco Aurélio Takita; Natália F. Martins

    2007-01-01

    Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under differe...

  9. An EST database from saffron stigmas

    OpenAIRE

    Chiusano Maria Luisa; Pizzichini Daniele; D'Agostino Nunzio; Giuliano Giovanni

    2007-01-01

    Abstract Background Saffron (Crocus sativus L., Iridaceae) flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part – the stigmas – are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. Results We produced 6,603 high quality Expressed Sequence Tags (ESTs) from a saffron stigma cDNA library. This collection is access...

  10. A Genome-Wide Survey of the Microsatellite Content of the Globe Artichoke Genome and the Development of a Web-Based Database.

    Science.gov (United States)

    Portis, Ezio; Portis, Flavio; Valente, Luisa; Moglia, Andrea; Barchi, Lorenzo; Lanteri, Sergio; Acquadro, Alberto

    2016-01-01

    The recently acquired genome sequence of globe artichoke (Cynara cardunculus var. scolymus) has been used to catalog the genome's content of simple sequence repeat (SSR) markers. More than 177,000 perfect SSRs were revealed, equivalent to an overall density across the genome of 244.5 SSRs/Mbp, but some 224,000 imperfect SSRs were also identified. About 21% of these SSRs were complex (two stretches of repeats separated by motifs. The SSRs were categorized for the numbers of repeats present, their overall length and were allocated to their linkage group. A total of 4,761 perfect and 6,583 imperfect SSRs were present in 3,781 genes (14.11% of the total), corresponding to an overall density across the gene space of 32,5 and 44,9 SSRs/Mbp for perfect and imperfect motifs, respectively. A putative function has been assigned, using the gene ontology approach, to the set of genes harboring at least one SSR. The same search parameters were applied to reveal the SSR content of 14 other plant species for which genome sequence is available. Certain species-specific SSR motifs were identified, along with a hexa-nucleotide motif shared only with the other two Compositae species (sunflower (Helianthus annuus) and horseweed (Conyza canadensis)) included in the study. Finally, a database, called "Cynara cardunculus MicroSatellite DataBase" (CyMSatDB) was developed to provide a searchable interface to the SSR data. CyMSatDB facilitates the retrieval of SSR markers, as well as suggested forward and reverse primers, on the basis of genomic location, genomic vs genic context, perfect vs imperfect repeat, motif type, motif sequence and repeat number. The SSR markers were validated via an in silico based PCR analysis adopting two available assembled transcriptomes, derived from contrasting globe artichoke accessions, as templates. PMID:27648830

  11. SPODOBASE : an EST database for the lepidopteran crop pest Spodoptera

    Directory of Open Access Journals (Sweden)

    Sabourault Cécile

    2006-06-01

    Full Text Available Abstract Background The Lepidoptera Spodoptera frugiperda is a pest which causes widespread economic damage on a variety of crop plants. It is also well known through its famous Sf9 cell line which is used for numerous heterologous protein productions. Species of the Spodoptera genus are used as model for pesticide resistance and to study virus host interactions. A genomic approach is now a critical step for further new developments in biology and pathology of these insects, and the results of ESTs sequencing efforts need to be structured into databases providing an integrated set of tools and informations. Description The ESTs from five independent cDNA libraries, prepared from three different S. frugiperda tissues (hemocytes, midgut and fat body and from the Sf9 cell line, are deposited in the database. These tissues were chosen because of their importance in biological processes such as immune response, development and plant/insect interaction. So far, the SPODOBASE contains 29,325 ESTs, which are cleaned and clustered into non-redundant sets (2294 clusters and 6103 singletons. The SPODOBASE is constructed in such a way that other ESTs from S. frugiperda or other species may be added. User can retrieve information using text searches, pre-formatted queries, query assistant or blast searches. Annotation is provided against NCBI, UNIPROT or Bombyx mori ESTs databases, and with GO-Slim vocabulary. Conclusion The SPODOBASE database provides integrated access to expressed sequence tags (EST from the lepidopteran insect Spodoptera frugiperda. It is a publicly available structured database with insect pest sequences which will allow identification of a number of genes and comprehensive cloning of gene families of interest for scientific community. SPODOBASE is available from URL: http://bioweb.ensam.inra.fr/spodobase

  12. A Genome-Wide Survey of the Microsatellite Content of the Globe Artichoke Genome and the Development of a Web-Based Database

    Science.gov (United States)

    Portis, Ezio; Portis, Flavio; Valente, Luisa; Moglia, Andrea; Barchi, Lorenzo; Lanteri, Sergio; Acquadro, Alberto

    2016-01-01

    The recently acquired genome sequence of globe artichoke (Cynara cardunculus var. scolymus) has been used to catalog the genome’s content of simple sequence repeat (SSR) markers. More than 177,000 perfect SSRs were revealed, equivalent to an overall density across the genome of 244.5 SSRs/Mbp, but some 224,000 imperfect SSRs were also identified. About 21% of these SSRs were complex (two stretches of repeats separated by <100 nt). Some 73% of the SSRs were composed of dinucleotide motifs. The SSRs were categorized for the numbers of repeats present, their overall length and were allocated to their linkage group. A total of 4,761 perfect and 6,583 imperfect SSRs were present in 3,781 genes (14.11% of the total), corresponding to an overall density across the gene space of 32,5 and 44,9 SSRs/Mbp for perfect and imperfect motifs, respectively. A putative function has been assigned, using the gene ontology approach, to the set of genes harboring at least one SSR. The same search parameters were applied to reveal the SSR content of 14 other plant species for which genome sequence is available. Certain species-specific SSR motifs were identified, along with a hexa-nucleotide motif shared only with the other two Compositae species (sunflower (Helianthus annuus) and horseweed (Conyza canadensis)) included in the study. Finally, a database, called “Cynara cardunculus MicroSatellite DataBase” (CyMSatDB) was developed to provide a searchable interface to the SSR data. CyMSatDB facilitates the retrieval of SSR markers, as well as suggested forward and reverse primers, on the basis of genomic location, genomic vs genic context, perfect vs imperfect repeat, motif type, motif sequence and repeat number. The SSR markers were validated via an in silico based PCR analysis adopting two available assembled transcriptomes, derived from contrasting globe artichoke accessions, as templates. PMID:27648830

  13. MELOGEN: an EST database for melon functional genomics

    Directory of Open Access Journals (Sweden)

    Puigdomènech Pere

    2007-09-01

    Full Text Available Abstract Background Melon (Cucumis melo L. is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs from eight normalized cDNA libraries from different tissues in different physiological conditions. Results We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs and 10,614 unclustered sequences (singletons. Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs and 356 single nucleotide polymorphisms (SNPs were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. Conclusion The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of

  14. Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptome

    OpenAIRE

    Kim Woo Taek; Cho Hye-Sun; Lee Bong-Woo; Kim JungEun; Lee Seung-Won; Baek Kwang-Hyun; Kim Hyun-Jin; Choi Doil; Hur Cheol-Goo

    2008-01-01

    Abstract Background There is no dedicated database available for Expressed Sequence Tags (EST) of the chili pepper (Capsicum annuum), although the interest in a chili pepper EST database is increasing internationally due to the nutritional, economic, and pharmaceutical value of the plant. Recent advances in high-throughput sequencing of the ESTs of chili pepper cv. Bukang have produced hundreds of thousands of complementary DNA (cDNA) sequences. Therefore, a chili pepper EST database was desi...

  15. Putative resistance genes in the CitEST database

    Directory of Open Access Journals (Sweden)

    Simone Guidetti-Gonzalez

    2007-01-01

    Full Text Available Disease resistance in plants is usually associated with the activation of a wide variety of defense responses to prevent pathogen replication and/or movement. The ability of the host plant to recognize the pathogen and to activate defense responses is regulated by direct or indirect interaction between the products of plant resistance (R and pathogen avirulence (Avr genes. Attempted infection of plants by avirulent pathogens elicits a battery of defenses often followed by the collapse of the challenged host cells. Localized host cell death may help to prevent the pathogen from spreading to uninfected tissues, known as hypersensitive response (HR. When either the plant or the pathogen lacks its cognate gene, activation of the plant’s defense responses fails to occur or is delayed and does not prevent pathogen colonization. In the CitEST database, we identified 1,300 reads related to R genes in Citrus which have been reported in other plant species. These reads were translated in silico, and alignments of their amino acid sequences revealed the presence of characteristic domains and motifs that are specific to R gene classes. The description of the reads identified suggests that they function as resistance genes in citrus.

  16. Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum transcriptome

    Directory of Open Access Journals (Sweden)

    Kim Woo Taek

    2008-10-01

    Full Text Available Abstract Background There is no dedicated database available for Expressed Sequence Tags (EST of the chili pepper (Capsicum annuum, although the interest in a chili pepper EST database is increasing internationally due to the nutritional, economic, and pharmaceutical value of the plant. Recent advances in high-throughput sequencing of the ESTs of chili pepper cv. Bukang have produced hundreds of thousands of complementary DNA (cDNA sequences. Therefore, a chili pepper EST database was designed and constructed to enable comprehensive analysis of chili pepper gene expression in response to biotic and abiotic stresses. Results We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO, and MIPS Functional Catalogue (FunCat. The Pepper EST database is designed to provide a workbench for (i identifying unigenes in pepper plants, (ii analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/. Conclusion The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.

  17. All 3' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...e name: CSV: kome_est_3end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_3end_al...p://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_3end_all.fasta.zip File size: 76 MB Simple search URL ...ST name SEQUENCE 3' EST sequence Joomla SEF URLs by Artio About This Database Database Description Download ...License Update History of This Database Site Policy | Contact Us All 3' EST - KOME | LSDB Archive ...

  18. All 5' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...e name: CSV: kome_est_5end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_5end_al...p://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_5end_all.fasta.zip File size: 27 MB Simple search URL ...ST name SEQUENCE 5' EST sequence Joomla SEF URLs by Artio About This Database Database Description Download ...License Update History of This Database Site Policy | Contact Us All 5' EST - KOME | LSDB Archive ...

  19. White mold of Jerusalem artichoke

    Science.gov (United States)

    Jerusalem artichoke (Helianthus tuberosus) is a Native American food plant closely related to the common sunflower (Helianthus annuus). Tubers of Jerusalem artichoke are increasingly available in retail grocery outlets. White mold (Sclerotinia stem rot), caused by the fungus, Sclerotinia sclerotioru...

  20. Details of rice EST mapping results - RGP estmap2001 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP estmap2001 Details... of rice EST mapping results Data detail Data name Details of rice EST mapping results... Map Image File Image file name of the rice transcript map Joomla SEF URLs by Artio About This Database Data...base Description Download License Update History of This Database Site Policy | Contact Us Details

  1. EST Table - KAIKOcDNA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available KAIKOcDNA EST Table Data detail Data name EST Table Description of data contents List of silkworm ESTs (cDNAs) consisting of public...ly available data in addition to sequences registered to public database as of Septem

  2. Download - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ClEST Download First of all, please read the license of this database. Data names and data descriptions are ...about the downloadable data in this page. They might not correspond to the contents of the original database... 3 Cluster clest_cluster.zip (525 KB) Simple search and download Downlaod via FTP Joomla SEF URLs by Artio About Thi...s Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - ClEST | LSDB Archive ...

  3. Construction, database integration, and application of an Oenothera EST library.

    Science.gov (United States)

    Mrácek, Jaroslav; Greiner, Stephan; Cho, Won Kyong; Rauwolf, Uwe; Braun, Martha; Umate, Pavan; Altstätter, Johannes; Stoppel, Rhea; Mlcochová, Lada; Silber, Martina V; Volz, Stefanie M; White, Sarah; Selmeier, Renate; Rudd, Stephen; Herrmann, Reinhold G; Meurer, Jörg

    2006-09-01

    Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches. PMID:16829020

  4. REDVET está indexada en Academia Journals Database

    Directory of Open Access Journals (Sweden)

    Redaccion Veterinaria.org

    2007-12-01

    Full Text Available El slogan de la Academia Journals Database, base de datos y directorio bibliográfico es “La difusión de conocimientos científicos de calidad controlada” por lo que es una satisfacción para REDVET que se nos valore por esta institución como una revista de calidad.

  5. License - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ClEST License License to Use This Database Last updated : 2012/05/22 You may use this database in compliance... with the terms and conditions of the license described below. The license specifies the license terms regarding the use of thi...s database and the requirements you must follow in using this ...database. The license for this database is specified in the Creative Commons Attribution-Share Alike 2.1 Jap

  6. Mining, characterization and validation of EST derived microsatellites from the transcriptome database of Allium sativum L

    OpenAIRE

    Chand, Subodh Kumar; Nanda, Satyabrata; Rout, Ellojita; Joshi, Raj Kumar

    2015-01-01

    Expressed Sequence Tags (ESTs) with comprehensive transcript information are valuable resources for development of molecular markers as they are derived from conserved genic regions. The present study highlights the mining of EST database to deduce the class I hyper variable SSRs in A. sativum. From 21694 garlic EST sequences, 642 non-redundant SSRs were identified with an average frequency of 1 per 14.9 kb of garlic transcriptome. The most abundant SSR motifs were the mononucleotides (32.86%...

  7. Clone - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ularius EST library Data file File name: clest_clone.zip File URL: ftp://ftp.biosciencedbc.jp/archive/clest/...e used for library construction Category Category of affiliation of sequence Cluster ID of cluster to which ...one Sequence Sequence Joomla SEF URLs by Artio About This Database Database Descr...iption Download License Update History of This Database Site Policy | Contact Us Clone - ClEST | LSDB Archive ...

  8. Database Description - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available arius EST Creator Creator Name: Minoru Moriyama* Creator Affiliation: National Institute of Advanced Industr...ctions Research Group, Bioproduction Research Institute, National Institute of Advanced Industrial Science a...otic Evolution and Biological Functions Research Group, Bioproduction Research Institute, National Institute of Advanced

  9. Database Description - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available Prot and TrEMBL databases. Although various plant genomes have been analyzed, the research concerning fern p...llus-veneris, which has been used for photomorphogenesis research, as a model fer... funding Research funded by the basic research budget of Tokyo Metropolitan University Reference(s) Article

  10. Fructans of Jerusalem artichokes

    DEFF Research Database (Denmark)

    Rumessen, J J; Bodé, S; Hamberg, O;

    1990-01-01

    Fructans are naturally occurring plant oligosaccharides with sweetening properties. Fructans (FAs) isolated from Jerusalem artichokes (Helianthus tuberosus) were studied with respect to intestinal handling and influence on blood glucose (BG), insulin, and C-peptide responses in eight healthy...... subjects. The responses were compared with those for fructose ingestion. The effect of FAs added to a wheat-starch meal was also studied. Standardized breath-hydrogen excretion indicated that FAs were completely malabsorbed and, after a 20-g dose, traces of FA were detected in 24-h urine collections in one...

  11. Download - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AcEST Download... First of all, please read the license of this database. Data names and data descriptions are about the download.... Click the links on Data Name for descriptions of the data. # Data name File Simple search and download...r annotation) acest_est.zip (110MB) Simple search and download 3 BlastX Result : Swiss-Prot blasthits_sp.zip... (7.77MB) Simple search and download 4 BlastX Result : TrEMBL blasthits_tr.zip (7

  12. About Libraries - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ry.zip File URL: ftp://ftp.biosciencedbc.jp/archive/acest/LATEST/acest_library.zi...p File size: 2KB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acest_library#en Data ...ription Download License Update History of This Database Site Policy | Contact Us About Libraries - AcEST | LSDB Archive ...

  13. Data correction on January 10th, 2013 - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available AcEST Data correction on January 10th, 2013 Errors regarding clone IDs and contig length were found on AcEST... Contig data, and they were corrected on January 10th, 2013. The contents of data correction are listed belo... of This Database Site Policy | Contact Us Data correction on January 10th, 2013 - AcEST | LSDB Archive ...

  14. Analysis of newly established EST databases reveals similarities between heart regeneration in newt and fish

    Directory of Open Access Journals (Sweden)

    Weis Patrick

    2010-01-01

    Full Text Available Abstract Background The newt Notophthalmus viridescens possesses the remarkable ability to respond to cardiac damage by formation of new myocardial tissue. Surprisingly little is known about changes in gene activities that occur during the course of regeneration. To begin to decipher the molecular processes, that underlie restoration of functional cardiac tissue, we generated an EST database from regenerating newt hearts and compared the transcriptional profile of selected candidates with genes deregulated during zebrafish heart regeneration. Results A cDNA library of 100,000 cDNA clones was generated from newt hearts 14 days after ventricular injury. Sequencing of 11520 cDNA clones resulted in 2894 assembled contigs. BLAST searches revealed 1695 sequences with potential homology to sequences from the NCBI database. BLAST searches to TrEMBL and Swiss-Prot databases assigned 1116 proteins to Gene Ontology terms. We also identified a relatively large set of 174 ORFs, which are likely to be unique for urodele amphibians. Expression analysis of newt-zebrafish homologues confirmed the deregulation of selected genes during heart regeneration. Sequences, BLAST results and GO annotations were visualized in a relational web based database followed by grouping of identified proteins into clusters of GO Terms. Comparison of data from regenerating zebrafish hearts identified biological processes, which were uniformly overrepresented during cardiac regeneration in newt and zebrafish. Conclusion We concluded that heart regeneration in newts and zebrafish led to the activation of similar sets of genes, which suggests that heart regeneration in both species might follow similar principles. The design of the newly established newt EST database allows identification of molecular pathways important for heart regeneration.

  15. AcEST(EST sequences of Adiantum capillus-veneris and their annotation) - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AcEST AcEST(EST sequence...s of Adiantum capillus-veneris and their annotation) Data detail Data name AcEST(EST sequence...s of Adiantum capillus-veneris and their annotation) Description of data contents EST sequence of A...db/view/archive_acest#en Data acquisition method Capillary sequencer Data analysi...atabases) Number of data entries Adiantum capillus-veneris ESTs: 30,540. Data item Description Clone id Clone ID of EST sequence

  16. Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database

    Institute of Scientific and Technical Information of China (English)

    Bao-Long NIU; Zhi-Qi MENG; Yue-Zhi TAO; Shun-Lin LU; Hong-Biao WENG; Li-Hua HE; Wei-Feng SHEN

    2005-01-01

    We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins.

  17. Mining, characterization and validation of EST derived microsatellites from the transcriptome database of Allium sativum L.

    Science.gov (United States)

    Chand, Subodh Kumar; Nanda, Satyabrata; Rout, Ellojita; Joshi, Raj Kumar

    2015-01-01

    Expressed Sequence Tags (ESTs) with comprehensive transcript information are valuable resources for development of molecular markers as they are derived from conserved genic regions. The present study highlights the mining of EST database to deduce the class I hyper variable SSRs in A. sativum. From 21694 garlic EST sequences, 642 non-redundant SSRs were identified with an average frequency of 1 per 14.9 kb of garlic transcriptome. The most abundant SSR motifs were the mononucleotides (32.86%) followed by trinucleotides (28.50%) and dinucleotides (13.39%). Among the individual SSRs, (A/T)n accounted for the highest number (137; 21.33%) followed by (G/C)n (74; 11.52%) and (AAG)n (63;9.81%). Primers designed from a robust set of 7 AsESTSSRs resulted in the amplification of 63 polymorphic alleles in 14 accessions of garlic. The resolving power of the markers varied from 4.286 (AsSSR7) to 18.143 (AsSSR13) while the average marker index (MI) was 5.087. These EST-SSRs markers for garlic could be useful for the improvement of garlic linkage map and could be used for evaluating genetic variation and comparative genomics studies in Allium species. PMID:25987765

  18. Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining

    Institute of Scientific and Technical Information of China (English)

    ZHAN Aibin; BAO Zhenmin; WANG Mingling; CHANG Dan; YUAN Jian; WANG Xiaolong; HU Xiaoli; LIANG Chengzhu; HU Jingjie

    2008-01-01

    The EST database of the Pacific abalone (Haliotis discus) was mined for developing mierosatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more mierosatellites. Based on the length and GC content of the flanking regions, duster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify seorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Hoand He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.

  19. Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP estmap2001 Statistics... information of rice EST mapping results Data detail Data name Statistics informati...of This Database Site Policy | Contact Us Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive ...

  20. An elm EST database for identifying leaf beetle egg-induced defense genes

    Directory of Open Access Journals (Sweden)

    Büchel Kerstin

    2012-06-01

    Full Text Available Abstract Background Plants can defend themselves against herbivorous insects prior to the onset of larval feeding by responding to the eggs laid on their leaves. In the European field elm (Ulmus minor, egg laying by the elm leaf beetle ( Xanthogaleruca luteola activates the emission of volatiles that attract specialised egg parasitoids, which in turn kill the eggs. Little is known about the transcriptional changes that insect eggs trigger in plants and how such indirect defense mechanisms are orchestrated in the context of other biological processes. Results Here we present the first large scale study of egg-induced changes in the transcriptional profile of a tree. Five cDNA libraries were generated from leaves of (i untreated control elms, and elms treated with (ii egg laying and feeding by elm leaf beetles, (iii feeding, (iv artificial transfer of egg clutches, and (v methyl jasmonate. A total of 361,196 ESTs expressed sequence tags (ESTs were identified which clustered into 52,823 unique transcripts (Unitrans and were stored in a database with a public web interface. Among the analyzed Unitrans, 73% could be annotated by homology to known genes in the UniProt (Plant database, particularly to those from Vitis, Ricinus, Populus and Arabidopsis. Comparative in silico analysis among the different treatments revealed differences in Gene Ontology term abundances. Defense- and stress-related gene transcripts were present in high abundance in leaves after herbivore egg laying, but transcripts involved in photosynthesis showed decreased abundance. Many pathogen-related genes and genes involved in phytohormone signaling were expressed, indicative of jasmonic acid biosynthesis and activation of jasmonic acid responsive genes. Cross-comparisons between different libraries based on expression profiles allowed the identification of genes with a potential relevance in egg-induced defenses, as well as other biological processes, including signal transduction

  1. EST2uni: an open, parallel tool for automated EST analysis and database creation, with a data mining web interface and microarray expression data integration

    Directory of Open Access Journals (Sweden)

    Nuez Fernando

    2008-01-01

    Full Text Available Abstract Background Expressed sequence tag (EST collections are composed of a high number of single-pass, redundant, partial sequences, which need to be processed, clustered, and annotated to remove low-quality and vector regions, eliminate redundancy and sequencing errors, and provide biologically relevant information. In order to provide a suitable way of performing the different steps in the analysis of the ESTs, flexible computation pipelines adapted to the local needs of specific EST projects have to be developed. Furthermore, EST collections must be stored in highly structured relational databases available to researchers through user-friendly interfaces which allow efficient and complex data mining, thus offering maximum capabilities for their full exploitation. Results We have created EST2uni, an integrated, highly-configurable EST analysis pipeline and data mining software package that automates the pre-processing, clustering, annotation, database creation, and data mining of EST collections. The pipeline uses standard EST analysis tools and the software has a modular design to facilitate the addition of new analytical methods and their configuration. Currently implemented analyses include functional and structural annotation, SNP and microsatellite discovery, integration of previously known genetic marker data and gene expression results, and assistance in cDNA microarray design. It can be run in parallel in a PC cluster in order to reduce the time necessary for the analysis. It also creates a web site linked to the database, showing collection statistics, with complex query capabilities and tools for data mining and retrieval. Conclusion The software package presented here provides an efficient and complete bioinformatics tool for the management of EST collections which is very easy to adapt to the local needs of different EST projects. The code is freely available under the GPL license and can be obtained at http://bioinf.comav.upv.es/est

  2. Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    Yang Jun-Bo

    2010-12-01

    Full Text Available Abstract Background The castor bean (Ricinus communis L., a monotypic species in the spurge family (Euphorbiaceae, 2n = 20, is an important non-edible oilseed crop widely cultivated in tropical, sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid (over 85% in its seed oil, the castor bean seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Due to lack of efficient molecular markers, little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular, expressed sequence tags (ESTs provide valuable resources to develop gene-associated SSR markers. Results In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean. Conclusion Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly

  3. Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters

    Directory of Open Access Journals (Sweden)

    Siddiqui Asim

    2005-10-01

    Full Text Available Abstract Background The sequencing and analysis of ESTs is for now the only practical approach for large-scale gene discovery and annotation in conifers because their very large genomes are unlikely to be sequenced in the near future. Our objective was to produce extensive collections of ESTs and cDNA clones to support manufacture of cDNA microarrays and gene discovery in white spruce (Picea glauca [Moench] Voss. Results We produced 16 cDNA libraries from different tissues and a variety of treatments, and partially sequenced 50,000 cDNA clones. High quality 3' and 5' reads were assembled into 16,578 consensus sequences, 45% of which represented full length inserts. Consensus sequences derived from 5' and 3' reads of the same cDNA clone were linked to define 14,471 transcripts. A large proportion (84% of the spruce sequences matched a pine sequence, but only 68% of the spruce transcripts had homologs in Arabidopsis or rice. Nearly all the sequences that matched the Populus trichocarpa genome (the only sequenced tree genome also matched rice or Arabidopsis genomes. We used several sequence similarity search approaches for assignment of putative functions, including blast searches against general and specialized databases (transcription factors, cell wall related proteins, Gene Ontology term assignation and Hidden Markov Model searches against PFAM protein families and domains. In total, 70% of the spruce transcripts displayed matches to proteins of known or unknown function in the Uniref100 database (blastx e-value Arabidopsis or rice genomes. Detailed analysis of translationally controlled tumour proteins and S-adenosylmethionine synthetase families confirmed a twofold size difference. Sequences and annotations were organized in a dedicated database, SpruceDB. Several search tools were developed to mine the data either based on their occurrence in the cDNA libraries or on functional annotations. Conclusion This report illustrates specific

  4. Computational EST database analysis identifies a novel member of the neuropoietic cytokine family.

    Science.gov (United States)

    Shi, Y; Wang, W; Yourey, P A; Gohari, S; Zukauskas, D; Zhang, J; Ruben, S; Alderson, R F

    1999-08-19

    A novel member of the neuropoietic cytokine family has been cloned and the protein expressed and characterized. In an effort to identify novel secreted proteins, an algorithm incorporating neural network algorithms was applied to a large EST database. A full-length clone was identified that is 1710 bp in length and has a single open reading frame of 225 amino acids. This new cytokine is most homologous to cardiotrophin-1, having a similarity and an identity of 46 and 29%, respectively, and therefore we have named it cardiotrophin-like cytokine (CLC). Northern hybridization analysis identified a 1.4-kb messenger RNA that is highly expressed in spleen and peripheral leukocytes. Purified recombinant CLC induced the activation of NFkappaB and SRE reporter constructs in the TF-1, U937, and M1 cell lines. Furthermore, the signal transduction pathway for CLC was characterized in the neuroblastoma cell line SK-N-MC and found to involve tyrosine phosphorylation of gp130 and STAT-1. PMID:10448081

  5. The barley EST DNA Replication and Repair Database (bEST-DRRD as a tool for the identification of the genes involved in DNA replication and repair

    Directory of Open Access Journals (Sweden)

    Gruszka Damian

    2012-06-01

    Full Text Available Abstract Background The high level of conservation of genes that regulate DNA replication and repair indicates that they may serve as a source of information on the origin and evolution of the species and makes them a reliable system for the identification of cross-species homologs. Studies that had been conducted to date shed light on the processes of DNA replication and repair in bacteria, yeast and mammals. However, there is still much to be learned about the process of DNA damage repair in plants. Description These studies, which were conducted mainly using bioinformatics tools, enabled the list of genes that participate in various pathways of DNA repair in Arabidopsis thaliana (L. Heynh to be outlined; however, information regarding these mechanisms in crop plants is still very limited. A similar, functional approach is particularly difficult for a species whose complete genomic sequences are still unavailable. One of the solutions is to apply ESTs (Expressed Sequence Tags as the basis for gene identification. For the construction of the barley EST DNA Replication and Repair Database (bEST-DRRD, presented here, the Arabidopsis nucleotide and protein sequences involved in DNA replication and repair were used to browse for and retrieve the deposited sequences, derived from four barley (Hordeum vulgare L. sequence databases, including the “Barley Genome version 0.05” database (encompassing ca. 90% of barley coding sequences and from two databases covering the complete genomes of two monocot models: Oryza sativa L. and Brachypodium distachyon L. in order to identify homologous genes. Sequences of the categorised Arabidopsis queries are used for browsing the repositories, which are located on the ViroBLAST platform. The bEST-DRRD is currently used in our project during the identification and validation of the barley genes involved in DNA repair. Conclusions The presented database provides information about the Arabidopsis genes involved in

  6. License - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available erivative work based on part of whole of the data from this database. 2. When you conduct research by using ...this database, and describe the research results in an article or paper, you alwa

  7. Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.).

    Science.gov (United States)

    Thiel, T; Michalek, W; Varshney, R K; Graner, A

    2003-02-01

    A software tool was developed for the identification of simple sequence repeats (SSRs) in a barley ( Hordeum vulgare L.) EST (expressed sequence tag) database comprising 24,595 sequences. In total, 1,856 SSR-containing sequences were identified. Trimeric SSR repeat motifs appeared to be the most abundant type. A subset of 311 primer pairs flanking SSR loci have been used for screening polymorphisms among six barley cultivars, being parents of three mapping populations. As a result, 76 EST-derived SSR-markers were integrated into a barley genetic consensus map. A correlation between polymorphism and the number of repeats was observed for SSRs built of dimeric up to tetrameric units. 3'-ESTs yielded a higher portion of polymorphic SSRs (64%) than 5'-ESTs did. The estimated PIC (polymorphic information content) value was 0.45 +/- 0.03. Approximately 80% of the SSR-markers amplified DNA fragments in Hordeum bulbosum, followed by rye, wheat (both about 60%) and rice (40%). A subset of 38 EST-derived SSR-markers comprising 114 alleles were used to investigate genetic diversity among 54 barley cultivars. In accordance with a previous, RFLP-based, study, spring and winter cultivars, as well as two- and six-rowed barleys, formed separate clades upon PCoA analysis. The results show that: (1) with the software tool developed, EST databases can be efficiently exploited for the development of cDNA-SSRs, (2) EST-derived SSRs are significantly less polymorphic than those derived from genomic regions, (3) a considerable portion of the developed SSRs can be transferred to related species, and (4) compared to RFLP-markers, cDNA-SSRs yield similar patterns of genetic diversity. PMID:12589540

  8. EuDBase: An online resource for automated EST analysis pipeline (ESTFrontier) and database for red seaweed Eucheuma denticulatum.

    Science.gov (United States)

    Hussein, Zeti Azura Mohamed; Loke, Kok Keong; Abidin, Rabiatul Adawiah Zainal; Othman, Roohaida

    2011-01-01

    Functional genomics has proven to be an efficient tool in identifying genes involved in various biological functions. However the availability of commercially important seaweed Eucheuma denticulatum functional resources is still limited. EuDBase is the first seaweed online repository that provides integrated access to ESTs of Eucheuma denticulatum generated from samples collected from Kudat and Semporna in Sabah, Malaysia. The database stored 10,031 ESTs that are clustered and assembled into 2,275 unique transcripts (UT) and 955 singletons. Raw data were automatically processed using ESTFrontier, an in-house automated EST analysis pipeline. Data was collected in MySQL database. Web interface is implemented using PHP and it allows browsing and querying EuDBase through search engine. Data is searchable via BLAST hit, domain search, Gene Ontology or KEGG Pathway. A user-friendly interface allows the identification of sequences either using a simple text query or similarity search. The development of EuDBase is initiated to store, manage and analyze the E. denticulatum ESTs and to provide accumulative digital resources for the use of global scientific community. EuDBase is freely available from http://www.inbiosis.ukm.my/eudbase/.

  9. Cluster - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...including Blastx search results and GO annotations Data file File name: clest_cluster.zip File URL: ftp://ftp.biosciencedbc.jp/archi... Consensus sequence Source Source clones No. of clones Number of source clones Top Blastx hit (E value t ACC No. Accession number of top Blastx hit E-value E-value of top Blastx hit ...GO GO annotation Joomla SEF URLs by Artio About This Database Database Description Download License Update History of Thi

  10. SSR mining in oil palm EST database: application in oil palm germplasm diversity studies

    Indian Academy of Sciences (India)

    Ngoot-Chin Ting; Noorhariza Mohd Zaki; Rozana Rosli; Eng-Ti Leslie Low; Maizura Ithnin; Suan-Choo Cheah; Soon-Guan Tan; Rajinder Singh

    2010-08-01

    This study reports on the detection of additional expressed sequence tags (EST) derived simple sequence repeat (SSR) markers for the oil palm. A large collection of 19243 Elaeis guineensis ESTs were assembled to give 10258 unique sequences, of which 629 ESTs were found to contain 722 SSRs with a variety of motifs. Dinucleotide repeats formed the largest group (45.6%) consisting of 66.9% AG/CT, 21.9% AT/AT, 10.9% AC/GT and 0.3% CG/CG motifs. This was followed by trinucleotide repeats, which is the second most abundant repeat types (34.5%) consisting of AAG/CTT (23.3%), AGG/CCT (13.7%), CCG/CGG (11.2%), AAT/ATT (10.8%), AGC/GCT (10.0%), ACT/AGT (8.8%), ACG/CGT (7.6%), ACC/GGT (7.2%), AAC/GTT (3.6%) and AGT/ACT (3.6%) motifs. Primer pairs were designed for 405 unique EST-SSRs and 15 of these were used to genotype 105 E. guineensis and 30 E. oleifera accessions. Fourteen SSRs were polymorphic in at least one germplasm revealing a total of 101 alleles. The high percentage (78.0%) of alleles found to be specific for either E. guineensis or E. oleifera has increased the power for discriminating the two species. The estimates of genetic differentiation detected by EST-SSRs were compared to those reported previously. The transferability across palm taxa to two Cocos nucifera and six exotic palms is also presented. The polymerase chain reaction (PCR) products of three primer-pairs detected in E. guineensis, E. oleifera, C. nucifera and Jessinia bataua were cloned and sequenced. Sequence alignments showed mutations within the SSR site and the flanking regions. Phenetic analysis based on the sequence data revealed that C. nucifera is closer to oil palm compared to J. bataua; consistent with the taxanomic classification.

  11. Exploiting EST databases for the mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L.

    Science.gov (United States)

    Joshi, Raj Kumar; Kar, Basudeba; Nayak, Sanghamitra

    2011-01-01

    Periwinkle (Catharanthus roseus L.) (Family: Apocyanaceae) is a ornamental plants with great medicinal properties. Although it is represented by seven species, little work has been carried out on its genetic characterization due to non-availability of reliable molecular markers. Simple sequence repeats (SSRs) have been widely applied as molecular markers in genetic studies. With the rapid increase in the deposition of nucleotide sequences in the public databases and advent of bioinformatics tools, it has become a cost effective and fast approach to scan for microsatellite repeats and exploit the possibility of converting it into potential genetic markers. Expressed sequence tags (EST's) from Catharanthus roseus were used for the screening of Class I (hyper variable) simple sequence repeats (SSR's). A total of 502 microsatellite repeats were detected from 21730 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs account to 1 SSR per 10.21 kb of EST. Mononucleotides was the most abundant class of microsatellite motifs. It accounted for 44.02% of the total, followed by the trinucleotide (26.09%) and dinucleotide repeats (14.34%). Among all the repeat motifs, (A/T)n accounted for the highest Proportion (36.25%) followed by (AAG)n. These detected SSRs can be used to design primers that have functional importance and should also facilitate the analysis of genetic diversity, variability, linkage mapping and evolutionary relationships in plants especially medicinal plants. PMID:21383904

  12. Comparative high-throughput transcriptome sequencing and development of SiESTa, the Silene EST annotation database

    Directory of Open Access Journals (Sweden)

    Marais Gabriel AB

    2011-07-01

    Full Text Available Abstract Background The genus Silene is widely used as a model system for addressing ecological and evolutionary questions in plants, but advances in using the genus as a model system are impeded by the lack of available resources for studying its genome. Massively parallel sequencing cDNA has recently developed into an efficient method for characterizing the transcriptomes of non-model organisms, generating massive amounts of data that enable the study of multiple species in a comparative framework. The sequences generated provide an excellent resource for identifying expressed genes, characterizing functional variation and developing molecular markers, thereby laying the foundations for future studies on gene sequence and gene expression divergence. Here, we report the results of a comparative transcriptome sequencing study of eight individuals representing four Silene and one Dianthus species as outgroup. All sequences and annotations have been deposited in a newly developed and publicly available database called SiESTa, the Silene EST annotation database. Results A total of 1,041,122 EST reads were generated in two runs on a Roche GS-FLX 454 pyrosequencing platform. EST reads were analyzed separately for all eight individuals sequenced and were assembled into contigs using TGICL. These were annotated with results from BLASTX searches and Gene Ontology (GO terms, and thousands of single-nucleotide polymorphisms (SNPs were characterized. Unassembled reads were kept as singletons and together with the contigs contributed to the unigenes characterized in each individual. The high quality of unigenes is evidenced by the proportion (49% that have significant hits in similarity searches with the A. thaliana proteome. The SiESTa database is accessible at http://www.siesta.ethz.ch. Conclusion The sequence collections established in the present study provide an important genomic resource for four Silene and one Dianthus species and will help to

  13. RNA-seq analysis and de novo transcriptome assembly of Jerusalem artichoke (Helianthus tuberosus Linne.

    Directory of Open Access Journals (Sweden)

    Won Yong Jung

    Full Text Available Jerusalem artichoke (Helianthus tuberosus L. has long been cultivated as a vegetable and as a source of fructans (inulin for pharmaceutical applications in diabetes and obesity prevention. However, transcriptomic and genomic data for Jerusalem artichoke remain scarce. In this study, Illumina RNA sequencing (RNA-Seq was performed on samples from Jerusalem artichoke leaves, roots, stems and two different tuber tissues (early and late tuber development. Data were used for de novo assembly and characterization of the transcriptome. In total 206,215,632 paired-end reads were generated. These were assembled into 66,322 loci with 272,548 transcripts. Loci were annotated by querying against the NCBI non-redundant, Phytozome and UniProt databases, and 40,215 loci were homologous to existing database sequences. Gene Ontology terms were assigned to 19,848 loci, 15,434 loci were matched to 25 Clusters of Eukaryotic Orthologous Groups classifications, and 11,844 loci were classified into 142 Kyoto Encyclopedia of Genes and Genomes pathways. The assembled loci also contained 10,778 potential simple sequence repeats. The newly assembled transcriptome was used to identify loci with tissue-specific differential expression patterns. In total, 670 loci exhibited tissue-specific expression, and a subset of these were confirmed using RT-PCR and qRT-PCR. Gene expression related to inulin biosynthesis in tuber tissue was also investigated. Exsiting genetic and genomic data for H. tuberosus are scarce. The sequence resources developed in this study will enable the analysis of thousands of transcripts and will thus accelerate marker-assisted breeding studies and studies of inulin biosynthesis in Jerusalem artichoke.

  14. Succinic acid production from Jerusalem artichoke

    DEFF Research Database (Denmark)

    Gunnarsson, Ingólfur Bragi; Karakashev, Dimitar Borisov; Angelidaki, Irini

    In this work, A. succinogenes 130Z was used to produce succinic acid from Jerusalem artichoke tuber hydrolysate. Results showed that both fructose and glucose in the tuber hydrolysate were utilized for succinic acid production. The sugar utilization was found to be dependent on process control......, hence, when pH was fixed at 6.8 the sugar utilization of fructose was increased from 68.6% to 96.5% and the succinic acid production was also increased by 26.4% to yield 26.8 g/L succinic acid. In this study a one-step pretreatment/hydrolysis method was used where no enzymes were used. Our work suggests...... that Jerusalem artichoke tubers could be utilized for production of bio-succinic acid....

  15. Stress -induced biosynthesis of dicaffeoylquinic acids in globe artichoke

    NARCIS (Netherlands)

    Moglia, A.; Lanteri, S.; Comino, C.; Acquadro, A.; Vos, de C.H.; Beekwilder, M.J.

    2008-01-01

    Leaf extracts from globe artichoke (Cynara cardunculus L. var. scolymus) have been widely used in medicine as hepatoprotectant and choleretic agents. Globe artichoke leaves represent a natural source of phenolic acids with dicaffeoylquinic acids, such as cynarin (1,3-dicaffeoylquinic acid), along wi

  16. Databases

    Data.gov (United States)

    National Aeronautics and Space Administration — The databases of computational and experimental data from the first Aeroelastic Prediction Workshop are located here. The databases file names tell their contents...

  17. Databases

    Digital Repository Service at National Institute of Oceanography (India)

    Kunte, P.D.

    Information on bibliographic as well as numeric/textual databases relevant to coastal geomorphology has been included in a tabular form. Databases cover a broad spectrum of related subjects like coastal environment and population aspects, coastline...

  18. EST sequences and their annotation (amino acid sequence and results of homology search) - Dicty_cDB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available and VS) derived from five developmental stages. Clone ID ID of cDNA clone Atlas ID ID of Atlas database ( ht...tp://dictycdb.biol.tsukuba.ac.jp/~tools/bin/ISH/index.html ) and link to Atlas database NBRP ID ID of cDNA c...ir annotations (amino acid sequence, homology search results (with target DBs: dicty EST-DB, DNA-DB and prot...ein-DB)). Links to the Atlas database ( http://dictycdb.biol.tsukuba.ac.jp/~tools/bin/ISH/index.html ), whic

  19. Identification and genetic mapping of highly polymorphic microsatellite loci from an EST database of the septoria tritici blotch pathogen Mycosphaerella graminicola

    NARCIS (Netherlands)

    Goodwin, S.B.; Lee, van der T.A.J.; Cavaletto, J.R.; Lintel Hekkert, te B.; Crane, C.F.; Kema, G.H.J.

    2007-01-01

    A database of 30,137 EST sequences from Mycosphaerella graminicola, the septoria tritici blotch fungus of wheat, was scanned with a custom software pipeline for di- and trinucleotide units repeated tandemly six or more times. The bioinformatics analysis identified 109 putative SSR loci, and for 99 o

  20. Genetic resources collections of leafy vegetables (lettuce, spinach, chicory, artichoke, asparagus, lamb’s lettuce, rhubarb and rocket salad): composition and gaps

    NARCIS (Netherlands)

    Treuren, van R.; Coquin, P.; Lohwasser, U.

    2012-01-01

    Lettuce, spinach and chicory are generally considered the main leafy vegetables, while a fourth group denoted by ‘minor leafy vegetables’ includes, amongst others, rocket salad, lamb’s lettuce, asparagus, artichoke and rhubarb. Except in the case of lettuce, central crop databases of leafy vegetable

  1. Databases

    Directory of Open Access Journals (Sweden)

    Nick Ryan

    2004-01-01

    Full Text Available Databases are deeply embedded in archaeology, underpinning and supporting many aspects of the subject. However, as well as providing a means for storing, retrieving and modifying data, databases themselves must be a result of a detailed analysis and design process. This article looks at this process, and shows how the characteristics of data models affect the process of database design and implementation. The impact of the Internet on the development of databases is examined, and the article concludes with a discussion of a range of issues associated with the recording and management of archaeological data.

  2. Hydrolase activity in Jerusalem artichoke and chicory

    Energy Technology Data Exchange (ETDEWEB)

    Klaushofer, H.; Abraham, B.; Leichtfried, G.

    1988-03-01

    Post-harvest storage of chicory and Jerusalem artichoke and overwintering of Jerusalem artichoke in the soil cause a more or less pronounced shortening of the fructan chain, depending on the variety. The proportion of fructose in the total fructan thus shifts towards glucose. This reduction on the fructose/glucose ratio is undesirable if the intention is to obtain a sweetener of high fructose content. In this work an attempt was made, via the quantity of fructose formed after a 4(3)-hour reaction of a tuber (root) extract with inulin, to assign a characteristic value to the depolymerization tendency of the material in question. However, since the plant extract not only contains enzymes (hydrolase A and B) that shorten the fructan chains but the activity of fructosyltransferase (SST, FFT) and enzymes of microbial origin (inulinase II, invertase) must also be considered, the concept of 'hydrolase activity' used by the authors is essentially an expression of 'total activity'. The activity unit (EU) is defined as the ability to split of 1 ..mu..mol of fructose from (chicory) inulin per minute under experimental conditions. Values of 0.25 to 0.77 EU/g dry solids were found in Jerusalem artichoke. Considerable differences may occur between varieties from the same cultivated area and the same harvest period. With one and the same variety, the activity appears to be subject to marked yearly fluctuations, so that at present, because of hydrolase activity, nothing certain can be said about the depolymerization tendency of a variety.

  3. New cropping designs for globe artichoke industry

    Directory of Open Access Journals (Sweden)

    Rosario Paolo Mauro

    2011-03-01

    Full Text Available A two-year experiment was carried-out in order to evaluate the effects of two plant arrangements (single vs. twin rows and four plant densities (1.0 -1.2 -1.4 and 1.8 plant m–2 on the agronomical behaviour and head characteristics of three globe artichoke genotypes (Violetto di Sicilia, Harmony F1 and Madrigal F1. The change of the cultivation format toward a high density stand significantly increase yield and yield synchronicity. The twin rows plant arrangement, although reduced total yield, increased the yield synchronicity. Moreover, the cultivation of seed-propagated genotypes (Harmony F1 and Madrigal F1 allowed extending significantly the availability of the heads across the year. On the basis of our results, we can assert that the implementation of a specific scheduling cultivation, based on higher density stands, twin rows plant arrangement and the integration of the traditional early genotypes with the new seed-propagated cultivars, is a promising way to match the requirements of a globe artichoke industrial crop, and to predispose a better mechanization of the cultural practices.

  4. Potential Biogas Production from Artichoke Byproducts in Sardinia, Italy

    OpenAIRE

    Fabio Menna; Remo Alessio Malagnino; Matteo Vittuari; Giovanni Molari; Giovanna Seddaiu; Paola A. Deligios; Stefania Solinas; Luigi Ledda

    2016-01-01

    The paper aims at evaluating the potential biogas production, both in terms of CH4 and theoretical energy potential, from globe artichoke agricultural byproducts in Sardinia. Field data about the productivity of byproducts were collected on five artichoke varieties cultivated in Sardinia, to assess the biomethane production of their aboveground non-food parts (excluding the head). Moreover, secondary data from previous studies and surveys at regional scale were collected to evaluate the poten...

  5. Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

    Directory of Open Access Journals (Sweden)

    Richardson Annette C

    2008-07-01

    Full Text Available Abstract Background Kiwifruit (Actinidia spp. are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs. Results The ESTs were derived mainly from four Actinidia species (A. chinensis, A. deliciosa, A. arguta and A. eriantha and fell into 41,858 non redundant clusters (18,070 tentative consensus sequences and 23,788 EST singletons. Analysis of flavor and fragrance-related gene families (acyltransferases and carboxylesterases and pathways (terpenoid biosynthesis is presented in comparison with a chemical analysis of the compounds present in Actinidia including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. Conclusion This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the Actinidia genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in Actinidia.

  6. Generation of a predicted protein database from EST data and application to iTRAQ analyses in grape (Vitis vinifera cv. Cabernet Sauvignon berries at ripening initiation

    Directory of Open Access Journals (Sweden)

    Smith Derek

    2009-01-01

    Full Text Available Abstract Background iTRAQ is a proteomics technique that uses isobaric tags for relative and absolute quantitation of tryptic peptides. In proteomics experiments, the detection and high confidence annotation of proteins and the significance of corresponding expression differences can depend on the quality and the species specificity of the tryptic peptide map database used for analysis of the data. For species for which finished genome sequence data are not available, identification of proteins relies on similarity to proteins from other species using comprehensive peptide map databases such as the MSDB. Results We were interested in characterizing ripening initiation ('veraison' in grape berries at the protein level in order to better define the molecular control of this important process for grape growers and wine makers. We developed a bioinformatic pipeline for processing EST data in order to produce a predicted tryptic peptide database specifically targeted to the wine grape cultivar, Vitis vinifera cv. Cabernet Sauvignon, and lacking truncated N- and C-terminal fragments. By searching iTRAQ MS/MS data generated from berry exocarp and mesocarp samples at ripening initiation, we determined that implementation of the custom database afforded a large improvement in high confidence peptide annotation in comparison to the MSDB. We used iTRAQ MS/MS in conjunction with custom peptide db searches to quantitatively characterize several important pathway components for berry ripening previously described at the transcriptional level and confirmed expression patterns for these at the protein level. Conclusion We determined that a predicted peptide database for MS/MS applications can be derived from EST data using advanced clustering and trimming approaches and successfully implemented for quantitative proteome profiling. Quantitative shotgun proteome profiling holds great promise for characterizing biological processes such as fruit ripening

  7. Analysis of EST database for two subspecies of largemouth bass%大口黑鲈两个亚种EST数据库分析

    Institute of Scientific and Technical Information of China (English)

    景燕娟; 白俊杰; 李胜杰; 于凌云; 蔡磊

    2012-01-01

    应用新一代高通量测序技术Roche 454对大口黑鲈(Micropterus salmoides)北方亚种和佛罗里达亚种进行转录组测序并建立ESTs数据库,结果得到北方亚种ESTs序列468 671条,佛罗里达亚种ESTs序列332 322条,两亚种ESTs序列的平均长度分别为306.5bp和304.4bp。将得到的高质量序列进行拼接,大口黑鲈北方亚种和佛罗里达亚种共得到contig序列总数分别为42 056条和35 743条,平均长度分别为612.6bp和588.2bp。将大口黑鲈北方亚种和佛罗里达亚种的数据库合并,通过与蛋白数据库比对后,共78 938条EST序列被注释,根据Gene Ontology(GO)信息,对序列按照分子功能、细胞组成、生物学过程进行分类。通过对大口黑鲈合并的EST库信息进行大通量SSR和SNP位点的发掘,发现了含SSRs和SNPs的序列分别为25 469和8 547条。从EST数据库中随机选取75条contigs进行北方亚种和佛罗里达亚种的序列比较,结果表明两个亚种EST序列同源性为99.2%。%In this study,Roche 454 high-throughput technology was used to conduct transcriptome sequencing and establish ESTs database of the largemouth bass northern subspecies and Florida subspecies.The results showed that a total of 468 671 and 332 322 ESTs were generated from northern subspecies and Florida subspecies.The average length was 306.5 bp and 304.4 bp,respectively.Assembly of the largemouth bass northern subspecies and Florida subspecies' high quality ESTs resulted in 42 056 and 35 743 contigs.The average length was 612.6 bp and 588.2 bp,respectively.EST database of largemouth bass northern subspecies and Florida subspecies was merged and then compared with known protein databases.78 938 EST sequences in total were annotated.By exploring the merged largemouth bass EST library,over 25 469 and 8 547 putative SSRs and SNPs were identified.Comparison of the northern subspecies and Florida subspecies sequence was made by randomly selecting 75 contigs from

  8. Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

    Science.gov (United States)

    Liu, Shengyi; Shi, Xuejie; Xu, Lanlan; Yi, Yuetao

    2016-03-01

    Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects of processing variables (extraction temperature, pH, extraction time, and liquid-to-solid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: pH 1.52, 63.62 min, 100°C and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52±0.90% was obtained, which validated the model prediction. The effects of diff erent drying methods (freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural diff erences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by diff erent methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin (SDP) and apple pectin (AP) were stronger than those of vacuum oven dried pectin (ODP) and vacuum freeze dried pectin (FDP). Therefore compared with the other two drying methods, the spray drying method was the best.

  9. Exploiting EST databases for the mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L.

    OpenAIRE

    Joshi, Raj Kumar; Kar, Basudeba; Nayak, Sanghamitra

    2011-01-01

    Periwinkle (Catharanthus roseus L.) (Family: Apocyanaceae) is a ornamental plants with great medicinal properties. Although it is represented by seven species, little work has been carried out on its genetic characterization due to non-availability of reliable molecular markers. Simple sequence repeats (SSRs) have been widely applied as molecular markers in genetic studies. With the rapid increase in the deposition of nucleotide sequences in the public databases and advent of bioinformatics t...

  10. Stress-induced biosynthesis of dicaffeoylquinic acids in globe artichoke.

    Science.gov (United States)

    Moglia, Andrea; Lanteri, Sergio; Comino, Cinzia; Acquadro, Alberto; de Vos, Ric; Beekwilder, Jules

    2008-09-24

    Leaf extracts from globe artichoke ( Cynara cardunculus L. var. scolymus) have been widely used in medicine as hepatoprotectant and choleretic agents. Globe artichoke leaves represent a natural source of phenolic acids with dicaffeoylquinic acids, such as cynarin (1,3-dicaffeoylquinic acid), along with its biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the most abundant molecules. This paper reports the development of an experimental system to induce caffeoylquinic acids. This system may serve to study the regulation of the biosynthesis of (poly)phenolic compounds in globe artichoke and the genetic basis of this metabolic regulation. By means of HPLC-PDA and accurate mass LC-QTOF MS and MS/MS analyses, the major phenolic compounds in globe artichoke leaves were identified: four isomers of dicaffeoylquinic acid, three isomers of caffeoylquinic acid, and the flavone luteolin 7-glucoside. Next, plant material was identified in which the concentration of phenolic compounds was comparable in the absence of particular treatments, with the aim to use this material to test the effect of stress application on the regulation of biosynthesis of caffeoylquinic acids. Using this material, the effect of UV-C, methyl jasmonate, and salicylic acid treatments on (poly)phenolic compounds was tested in different globe artichoke genotypes. UV-C exposure consistently increased the levels of dicaffeoylquinic acids in all genotypes, whereas the effect on compounds from the same biosynthetic pathway, for example, chlorogenic acid and luteolin-7-glucoside, was much less pronounced and was not statistically significant. No effect of methyl jasmonate or salicylic acid was found. Time-response experiments indicated that the level of dicaffeoylquinic acids reached a maximum at 24 h after UV radiation. On the basis of these results a role of dicaffeoylquinic acids in UV protection in globe artichoke is hypothesized. PMID:18710252

  11. Annotation of novel neuropeptide precursors in the migratory locust based on transcript screening of a public EST database and mass spectrometry

    Directory of Open Access Journals (Sweden)

    De Loof Arnold

    2006-08-01

    Full Text Available Abstract Background For holometabolous insects there has been an explosion of proteomic and peptidomic information thanks to large genome sequencing projects. Heterometabolous insects, although comprising many important species, have been far less studied. The migratory locust Locusta migratoria, a heterometabolous insect, is one of the most infamous agricultural pests. They undergo a well-known and profound phase transition from the relatively harmless solitary form to a ferocious gregarious form. The underlying regulatory mechanisms of this phase transition are not fully understood, but it is undoubtedly that neuropeptides are involved. However, neuropeptide research in locusts is hampered by the absence of genomic information. Results Recently, EST (Expressed Sequence Tag databases from Locusta migratoria were constructed. Using bioinformatical tools, we searched these EST databases specifically for neuropeptide precursors. Based on known locust neuropeptide sequences, we confirmed the sequence of several previously identified neuropeptide precursors (i.e. pacifastin-related peptides, which consolidated our method. In addition, we found two novel neuroparsin precursors and annotated the hitherto unknown tachykinin precursor. Besides one of the known tachykinin peptides, this EST contained an additional tachykinin-like sequence. Using neuropeptide precursors from Drosophila melanogaster as a query, we succeeded in annotating the Locusta neuropeptide F, allatostatin-C and ecdysis-triggering hormone precursor, which until now had not been identified in locusts or in any other heterometabolous insect. For the tachykinin precursor, the ecdysis-triggering hormone precursor and the allatostatin-C precursor, translation of the predicted neuropeptides in neural tissues was confirmed with mass spectrometric techniques. Conclusion In this study we describe the annotation of 6 novel neuropeptide precursors and the neuropeptides they encode from the

  12. New Polylactic Acid Composites Reinforced with Artichoke Fibers

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    Luigi Botta

    2015-11-01

    Full Text Available In this work, artichoke fibers were used for the first time to prepare poly(lactic acid (PLA-based biocomposites. In particular, two PLA/artichoke composites with the same fiber loading (10% w/w were prepared by the film-stacking method: the first one (UNID reinforced with unidirectional long artichoke fibers, the second one (RANDOM reinforced by randomly-oriented long artichoke fibers. Both composites were mechanically characterized in tensile mode by quasi-static and dynamic mechanical tests. The morphology of the fracture surfaces was analyzed through scanning electron microscopy (SEM. Moreover, a theoretical model, i.e., Hill’s method, was used to fit the experimental Young’s modulus of the biocomposites. The quasi-static tensile tests revealed that the modulus of UNID composites is significantly higher than that of the neat PLA (i.e., ~40%. Moreover, the tensile strength is slightly higher than that of the neat matrix. The other way around, the stiffness of RANDOM composites is not significantly improved, and the tensile strength decreases in comparison to the neat PLA.

  13. Potential Biogas Production from Artichoke Byproducts in Sardinia, Italy

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    Fabio De Menna

    2016-02-01

    Full Text Available The paper aims at evaluating the potential biogas production, both in terms of CH4 and theoretical energy potential, from globe artichoke agricultural byproducts in Sardinia. Field data about the productivity of byproducts were collected on five artichoke varieties cultivated in Sardinia, to assess the biomethane production of their aboveground non-food parts (excluding the head. Moreover, secondary data from previous studies and surveys at regional scale were collected to evaluate the potential biogas production of the different districts. Fresh globe artichoke residues yielded, on average, 292.2 Nm3·tDOM−1, with dissimilarities among cultivars. Fresh samples were analyzed in two series: (a wet basis; and (b wet basis with catalytic enzymes application. Enzymes proved to have some beneficial effects in terms of anticipated biomethane availability. At the regional level, ab. 20 × 106 Nm3 CH4 could be produced, corresponding to the 60% of current installed capacity. However, districts potentials show some differences, depending on the specific biomass partitioning and on the productivity of cultivated varieties. Regional assessments should encompass the sensitiveness of results to agro-economic variables and the economic impacts of globe artichoke residue use in the current regional biogas sector.

  14. Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L.).

    Science.gov (United States)

    López-Molina, Dorotea; Navarro-Martínez, María Dolores; Rojas Melgarejo, Francisco; Hiner, Alexander N P; Chazarra, Soledad; Rodríguez-López, José Neptuno

    2005-06-01

    A high molecular weight inulin has been prepared from artichoke (Cynara scolymus L.) agroindustrial wastes using environmentally benign aqueous extraction procedures. Physico-chemical analysis of the properties of artichoke inulin was carried out. Its average degree of polymerization was 46, which is higher than for Jerusalem artichoke, chicory, and dahlia inulins. GC-MS confirmed that the main constituent monosaccharide in artichoke inulin was fructose and its degradation by inulinase indicated that it contained the expected beta-2,1-fructan bonds. The FT-IR spectrum was identical to that of chicory inulin. These data indicate that artichoke inulin will be suitable for use in a wide range of food applications. The health-promoting prebiotic effects of artichoke inulin were demonstrated in an extensive microbiological study showing a long lasting bifidogenic effect on Bifidobacterium bifidum ATCC 29521 cultures and also in mixed cultures of colonic bacteria.

  15. Dynamics of flowering of artichoke globe (Cynara scolymus L.) plants in depending on cultivation method

    OpenAIRE

    Andrzej Sałata

    2012-01-01

    The study was carried out in the years 2001-2002 in the Felin Research Center in Lublin. The differences in growth dynamics and morphology of flowering shoots were investigated with regards to a method of cultivation of artichoke. In the year 2001 flowering shoots and flowers occurred the earliest in the year 2001 on artichoke plants cultivated from crowns. In the year 2002 plants cultivated from crowns and transplants produced flowering shoots in the same time. Artichoke plants cultivated fr...

  16. The Possible Efficacy of Artichoke in Fluconazole Related Hepatotoxicity

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    Hüseyin Kurt

    2014-01-01

    Full Text Available Although fluconazole related hepatotoxicity (FRH is rare, mortal acute hepatic necrosis and jaundice were reported in immunocompromised states such as acquired immunodeficiency syndrome (AIDS and bone marrow transplant (BMT. We present a case of a patient with multiple sclerosis who developed hepatotoxicity with the use of a single 150 mg fluconazole tablet for fungal vaginitis, 10 days after methylprednisolone pulse treatment. Our patient’s alanine aminotransferase (ALT and aspartate aminotransferase (AST levels were decreased, 1200 U/L and 800 U/L, respectively, and bilirubin levels were consistent at 37 mg/dL. Artichoke which has anticholestatic and antioxidant properties was used by our patient. She consumed a 30 mg artichoke leaf extract tea 3 times a day. The bilirubin levels significantly declined at the end of the first week and all liver function tests were normalized within 2 months.

  17. Influence of planting date and temperature on inulin content in Jerusalem Artichoke (Helianthus tuberosus L.)

    Science.gov (United States)

    Lower temperatures during the dry season in tropical regions might affect inulin content and inulin yield of Jerusalem artichoke. The objective of this study was to determine the effect of planting dates during low temperature on inulin yield and content of Jerusalem artichoke. Two pot experiments...

  18. Accumulation of cynaropicrin in globe artichoke and localization ofenzymes involved in its biosynthesis

    NARCIS (Netherlands)

    Eljounaidi, K.; Comino, C.; Moglia, A.; Cankar, K.; Genre, A.; Hehn, A.; Bourgaud, F.; Beekwilder, J.; Lanteri, S.

    2015-01-01

    Globe artichoke (Cynara cardunculus var. scolymus) belongs to the Asteraceae family, in which one ofthe most biologically significant class of secondary metabolites are sesquiterpene lactones (STLs). Inglobe artichoke the principal STL is the cynaropicrin, which contributes to approximately 80% of i

  19. Artichoke (Cynara scolymus L. as cash-cover crop in an organic vegetable system

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    Anna LENZI

    2015-11-01

    Full Text Available In organic vegetable systems green manure crops play an important role as a nitrogen source, but they cover the soil for several months without producing a direct income. Globe artichoke (Cynara scolymus L. provides both heads to be harvested and particularly abundant plant residues to be possibly incorporated into the soil, so it may play a double role of cash and cover crop. This paper describes an on-farm study in which seed-propagated artichoke, cultivated as an annual crop, preceded zucchini squash and lettuce cultivated in sequence within a vegetable organic system. Artichoke produced about 7 t ha-1 of saleable heads and left, after harvest, 50.3 t ha-1 of fresh biomass usable as green manure. Zucchini squash and lettuce following artichoke showed a significant increase in yield when artichoke residues were incorporated into the soil. Furthermore, a residual positive effect of green manure on soil fertility was detected after lettuce harvest. 

  20. Influence of sugars on the dry-weight increase of gamma irradiated Jerusalem artichoke tuber's tissue

    International Nuclear Information System (INIS)

    Jerusalem artichoke tuber's explants after a γ irradiation of 6000 rads couldn't proliferate in a growth medium containing glucose and indolyl acetic acid; their dry weight is increased by an accumulation of sugars

  1. Sensory quality and appropriateness of raw and boiled Jerusalem artichoke tubers (Helianthus tuberosus L.)

    DEFF Research Database (Denmark)

    Bach, Vibe; Kidmose, Ulla; Thybo, Anette;

    2013-01-01

    BACKGROUND: The aim of the present study was to investigate the sensory attributes, dry matter and sugar content of five varieties of Jerusalem artichoke tubers and their relation to the appropriateness of the tubers for raw and boiled preparation. RESULTS: Sensory evaluation of raw and boiled...... Jerusalem artichoke tubers was performed by a trained sensory panel and a semi-trained consumer panel of 49 participants, who also evaluated the appropriateness of the tubers for raw and boiled preparation. The appropriateness of raw Jerusalem artichoke tubers was related to Jerusalem artichoke flavour......, green nut flavour, sweetness and colour intensity, whereas the appropriateness of boiled tubers was related to celeriac aroma, sweet aroma, sweetness and colour intensity. In both preparations the variety Dwarf stood out from the others by being the least appropriate tuber. CONCLUSION: A few sensory...

  2. The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production

    OpenAIRE

    Linxi Yang; Quan Sophia He; Kenneth Corscadden; Udenigwe, Chibuike C.

    2015-01-01

    Jerusalem artichoke, a native plant to North America has recently been recognized as a promising biomass for bioeconomy development, with a number of advantages over conventional crops such as low input cultivation, high crop yield, wide adaptation to climatic and soil conditions and strong resistance to pests and plant diseases. A variety of bioproducts can be derived from Jerusalem artichoke, including inulin, fructose, natural fungicides, antioxidant and bioethanol. This paper provides an ...

  3. A Shortcut to the Production of High Ethanol Concentration from Jerusalem Artichoke Tubers

    OpenAIRE

    Ge, Xiang-Yang; Zhang, Wei-Guo

    2005-01-01

    Aspergillus niger SL-09, a newly isolated exoinulinase-hyperproducing strain, and Saccharomyces cerevisiae Z-06, with high ethanol tolerance, were used in a fed-batch process for simultaneous saccharification and fermentation of Jerusalem artichoke tuber mash and flour. S. cerevisiae Z-06 utilized 98 % of the total sugar and produced 19.6 % of ethanol in 48 h. In this process the conversion efficiency of the fermentation of Jerusalem artichoke and the production of ethanol were 90 % of the th...

  4. The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway

    Directory of Open Access Journals (Sweden)

    Bourgaud Frédéric

    2009-03-01

    Full Text Available Abstract Background The leaves of globe artichoke and cultivated cardoon (Cynara cardunculus L. have significant pharmaceutical properties, which mainly result from their high content of polyphenolic compounds such as monocaffeoylquinic and dicaffeoylquinic acid (DCQ, and a range of flavonoid compounds. Results Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase (HQT encoding genes have been isolated from both globe artichoke and cultivated cardoon (GenBank accessions DQ915589 and DQ915590, respectively using CODEHOP and PCR-RACE. A phylogenetic analysis revealed that their sequences belong to one of the major acyltransferase groups (anthranilate N-hydroxycinnamoyl/benzoyltransferase. The heterologous expression of globe artichoke HQT in E. coli showed that this enzyme can catalyze the esterification of quinic acid with caffeoyl-CoA or p-coumaroyl-CoA to generate, respectively, chlorogenic acid (CGA and p-coumaroyl quinate. Real time PCR experiments demonstrated an increase in the expression level of HQT in UV-C treated leaves, and established a correlation between the synthesis of phenolic acids and protection against damage due to abiotic stress. The HQT gene, together with a gene encoding hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT previously isolated from globe artichoke, have been incorporated within the developing globe artichoke linkage maps. Conclusion A novel acyltransferase involved in the biosynthesis of CGA in globe artichoke has been isolated, characterized and mapped. This is a good basis for our effort to understand the genetic basis of phenylpropanoid (PP biosynthesis in C. cardunculus.

  5. Artichoke, Cynarin and Cyanidin Downregulate the Expression of Inducible Nitric Oxide Synthase in Human Coronary Smooth Muscle Cells

    Directory of Open Access Journals (Sweden)

    Ning Xia

    2014-03-01

    Full Text Available Artichoke (Cynara scolymus L. is one of the world’s oldest medicinal plants with multiple health benefits. We have previously shown that artichoke leaf extracts and artichoke flavonoids upregulate the gene expression of endothelial-type nitric oxide synthase (eNOS in human endothelial cells. Whereas NO produced by the eNOS is a vasoprotective molecule, NO derived from the inducible iNOS plays a pro-inflammatory role in the vasculature. The present study was aimed to investigate the effects of artichoke on iNOS expression in human coronary artery smooth muscle cells (HCASMC. Incubation of HCASMC with a cytokine mixture led to an induction of iNOS mRNA expression. This iNOS induction was concentration- and time-dependently inhibited by an artichoke leaf extract (1–100 µg/mL, 6 h or 24 h. Consistently, the artichoke leaf extract also reduced cytokine-induced iNOS promoter activation and iNOS protein expression. In addition, treatment of HCASMC with four well-known artichoke compounds (cynarin > cyanidin > luteolin ≈ cynaroside led to a downregulation iNOS mRNA and protein expression, with cynarin being the most potent one. In conclusion, artichoke contains both eNOS-upregulating and iNOS-downregulating compounds. Such compounds may contribute to the beneficial effects of artichoke and may per se have therapeutic potentials.

  6. Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.

    Science.gov (United States)

    Xia, Ning; Pautz, Andrea; Wollscheid, Ursula; Reifenberg, Gisela; Förstermann, Ulrich; Li, Huige

    2014-01-01

    Artichoke (Cynara scolymus L.) is one of the world's oldest medicinal plants with multiple health benefits. We have previously shown that artichoke leaf extracts and artichoke flavonoids upregulate the gene expression of endothelial-type nitric oxide synthase (eNOS) in human endothelial cells. Whereas NO produced by the eNOS is a vasoprotective molecule, NO derived from the inducible iNOS plays a pro-inflammatory role in the vasculature. The present study was aimed to investigate the effects of artichoke on iNOS expression in human coronary artery smooth muscle cells (HCASMC). Incubation of HCASMC with a cytokine mixture led to an induction of iNOS mRNA expression. This iNOS induction was concentration- and time-dependently inhibited by an artichoke leaf extract (1-100 µg/mL, 6 h or 24 h). Consistently, the artichoke leaf extract also reduced cytokine-induced iNOS promoter activation and iNOS protein expression. In addition, treatment of HCASMC with four well-known artichoke compounds (cynarin > cyanidin > luteolin ≈ cynaroside) led to a downregulation iNOS mRNA and protein expression, with cynarin being the most potent one. In conclusion, artichoke contains both eNOS-upregulating and iNOS-downregulating compounds. Such compounds may contribute to the beneficial effects of artichoke and may per se have therapeutic potentials. PMID:24662080

  7. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats

    DEFF Research Database (Denmark)

    Jimenez-Escrig, A.; Dragsted, Lars Ove; Daneshvar, Bahram;

    2003-01-01

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2......,2-diphenyl-1-picrylhydrazyl (DPPH.) scavenging, (b) ferric-reducing antioxidant power (FRAP), and...

  8. Kombucha fermentation on raw extracts of different cultivars of Jerusalem artichoke

    Directory of Open Access Journals (Sweden)

    Lončar Eva S.

    2007-01-01

    Full Text Available Kombucha is a symbiosis between yeasts and acetic bacteria. It usually grows on sweetened black tea, but cultivation is possible on many other substrates. Jerusalem artichoke tubers extract is one of them. Tubers are suitable for the dietetic nutrition because of the low monosaccharide content and presence of some polyfructan ingredients which act as prebiotic. Five different cultivars of Jerusalem artichoke were used for the preparation of substrates for kombucha fermentation. The aim of this paper was the investigation of the influence of different Jerusalem artichoke cultivars on metabolic activity of kombucha. Composition of carbohydrates was followed using thin-layer chromatography and pH, reducing sugars content and yield of biomass were measured. Most of the samples with Jerusalem artichoke tubers extract contained fructose, probably small amount of glucose, fructo-oligosaccharides with different degree of polymerization and, inulin. Considering TLC chromatograms, Jerusalem artichoke cultivar did not affect significantly the composition of oligosaccharides in the fermentative liquid, as only minor differences were observed.

  9. Study on exploitation of Jerusalem artichoke bulbs in relation to chemical composition

    Directory of Open Access Journals (Sweden)

    Sorina Ropciuc

    2014-11-01

    Full Text Available Abstract Jerusalem artichoke (Helianthus tuberosus L. known in USA also as sunchoke, is a perennial plant, well-adapted to humid and cold climats, nonpretencious to soil and with good yield increase. The bulbs are the eatable part that grows in the ground that have certain similitudes with the potato. The value of Jerusalem artichoke as a technical and medical vegetable is based on the chemical composition of the plant. The bulbs of the Jerusalem artichoke harvested in autumn have been sensorially and chemically analyzed along the deposition during the cold season. Measurements were made on the light brown variety with a slightly elongated shape. The low mass loss during depostion, the high content of reducing sugar (4.7- 6.12% and total carbohydrates (94.27-96.18% enable their successful use in the functional food and for the production of alcohol.

  10. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L.) in a Biorefinery Concept

    DEFF Research Database (Denmark)

    Johansson, Eva; Prade, Thomas; Angelidaki, Irini;

    2015-01-01

    Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L.) is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper...... focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals...

  11. Development of Novel, Exon-Primed Intron-Crossing (EPIC Markers from EST Databases and Evaluation of their Phylogenetic Utility in Commiphora (Burseraceae

    Directory of Open Access Journals (Sweden)

    Morgan R. Gostel

    2014-03-01

    Full Text Available Premise of the study: Novel nuclear exon-primed intron-crossing (EPIC markers were developed to increase phylogenetic resolution among recently diverged lineages in the frankincense and myrrh family, Burseraceae, using Citrus, Arabidopsis, and Oryza genome resources. Methods and Results: Primer pairs for 48 nuclear introns were developed using the genome resource IntrEST and were screened using species of Commiphora and other Burseraceae taxa. Four putative intron regions (RPT6A, BXL2, mtATP Synthase D, and Rab6 sequenced successfully for multiple taxa and recovered phylogenies consistent with those of existing studies. In some cases, these regions yielded informative sequence variation on par with that of the nuclear ribosomal DNA internal transcribed spacer. Conclusions: The combination of freely available genome resources and our design criteria have uncovered four single-copy nuclear intron regions that are useful for phylogenetic reconstruction of Burseraceae taxa. Because our EPIC primers also amplify Arabidopsis, we recommend their trial in other rosid and eudicot lineages.

  12. Growth and yield performance of Jerusalem artichoke clones in a semiarid region of China

    NARCIS (Netherlands)

    Liu, Z.X.; Spiertz, J.H.J.; Sha, J.; Xue, S.; Xie, G.H.

    2012-01-01

    This study investigated biomass yield and growth characteristics of 26 Jerusalem artichoke (Helianthus tuberosus L.) clones and assessed it as a bioenergy crop for a semiarid region of the Loess Plateau in China. Genotype, year, and genotype ´ year interaction contributed to differences in crop deve

  13. CHANGE OF INULIN IN THE TUBERS OF JERUSALEM ARTICHOKE AT STORAGE

    Directory of Open Access Journals (Sweden)

    Nazarenko M. N.

    2013-12-01

    Full Text Available This article examines the change of the mass fraction of inulin in Jerusalem artichoke tubers during storage under different conditions. The influence of temperature, storage time and variety features on the content of inulin have been shown. The terms and conditions of storage of raw materials before processing have been set.

  14. Cadmium Accumulation and Translocation in Two Jerusalem Artichoke (Helianthus tuberosus L.) Cultivars

    Institute of Scientific and Technical Information of China (English)

    CHEN Liang; LONG Xiao-Hua; ZHANG Zhen-Hua; ZHENG Xiao-Tao; Z. RENGEL; LIU Zhao-Pu

    2011-01-01

    Jerusalem artichoke (Helianthus tuberosus L.) not just can be used for bioethanol production but may be potentially used in phytoremediation for the removal of heavy metal pollutants.Two Jerusalem artichoke cultivars,N2 and N5,were subjected to six cadmium (Cd) concentrations (0,5,25,50,100 and 200 mg L-1) to investigate Cd tolerance and accumulation.After 21 days of growth,the effects of Cd on growth,chlorophyll content,net photosynthetic rate,intercellular CO2 concentration and malondialdehyde content were evaluated.Most growth parameters were reduced under Cd stress.The two Jerusalem artichoke cultivars had relatively high Cd tolerance and accumulation capacity (> 100 mg kg-1),with N5 being more tolerant and having higher Cd accumulation than N2.Roots accumulated more Cd than stems and leaves.The bioconcentration factors (far higher than 1) and translocation factors (lower than 1) decreased with an increase in Cd applied.The results suggested that Jerusalem artichoke could be grown at relatively high Cd loads,and N5 could be an excellent candidate for phytoremediation of Cd-contaminated soils.

  15. In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

    NARCIS (Netherlands)

    Menin, B.; Moglia, A.; Comino, C.; Lanteri, S.; Herpen, van T.W.J.M.; Beekwilder, M.J.

    2012-01-01

    Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We

  16. Design and Simulation of Two Robotic Systems for Automatic Artichoke Harvesting

    Directory of Open Access Journals (Sweden)

    Domenico Longo

    2013-12-01

    Full Text Available The target of this research project was a feasibility study for the development of a robot for automatic or semi-automatic artichoke harvesting. During this project, different solutions for the mechanical parts of the machine, its control system and the harvesting tools were investigated. Moreover, in cooperation with the department DISPA of University of Catania, different field structures with different kinds of artichoke cultivars were studied and tested. The results of this research could improve artichoke production for preserves industries. As a first step, an investigation on existing machines has been done. From this research, it has been shown that very few machines exist for this purpose. Based also on previous experiences, some proposals for different robotic systems have been done, while the mobile platform itself was developed within another research project. At the current stage, several different configurations of machines and harvesting end-effectors have been designed and simulated using a 3D CAD environment interfaced with Matlab®. Moreover, as support for one of the proposed machines, an artificial vision algorithm has been developed in order to locate the artichokes on the plant, with respect to the robot, using images taken with a standard webcam.

  17. El potencial del topinambur en la salud y la nutrición The potential of Jerusalem Artichokes in health and nutrition

    Directory of Open Access Journals (Sweden)

    D Scollo

    2011-12-01

    Full Text Available Introducción: El topinambur es un tubérculo con alto contenido en inulina. La inulina es un carbohidrato soluble no digerible que está presente en muchos vegetales, frutas y cereales siendo usada ampliamente como ingrediente en alimentos funcionales. Objetivo: Obtener tubérculos de topinambur, fijar distintos parámetros de extracción de inulina y preparar diversos alimentos, reemplazando parte de las harinas tradicionales por harina de topinambur. Se propuso, además, evaluar la preferencia de los consumidores sobre los mismos. Material y métodos: Se sembraron y cosecharon tubérculos de topinambur partiendo de 4 parcelas con diferentes condiciones de riego y fertilización. Los mismos se conservaron a variadas temperaturas. Se determinó el contenido de inulina por HPLC y se realizó la extracción de la misma de los tubérculos. A partir de los tubérculos cosechados en la parcela 3 se elaboraron harinas que fueron utilizadas para reemplazar parcialmente a la harina de trigo en la preparación de diferentes productos alimenticios. Sobre los mismos se realizó una evaluación de preferencia. Resultados: El rinde de la cosecha fue mayor en la parcela 3 (tierra fértil y riego según necesidades, 91 ton/Ha. El método de conservación más adecuado fue el frío, sin embargo no se utilizaron estos tubérculos para la extracción de inulina. Los productos alimenticios obtenidos fueron considerados adecuados por los jueces intervinientes. Conclusiones: la elaboración de productos alimenticios a partir de harina de topinambur podría ser una alternativa de alimento funcional por su contenido de inulina.Introduction: The Jerusalem artichoke Is a tuber high in inulin. Inulin is a soluble non-digestible carbohydrate that is present in many vegetables, fruits and cereals and is widely used as an ingredient in functional foods. Objective: To get Jerusalem artichoke tubers, set various parameters for extraction of inulin and prepare various foods

  18. EST2Prot: Mapping EST sequences to proteins

    Directory of Open Access Journals (Sweden)

    Lin David M

    2006-03-01

    Full Text Available Abstract Background EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. Results We describe a system (EST2Prot that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. Conclusion EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at http://biozon.org/tools/est/.

  19. Variation of Phenolic Content in Globe Artichoke in Relation to Biological, Technical and Environmental Factors

    Directory of Open Access Journals (Sweden)

    Giovanni Mauromicale

    2011-02-01

    Full Text Available In Italy, globe artichoke production is prevailingly concentrated in the South and islands, where it provides an important contribution to the agricultural economy. In recent years, there has been a renewed interest in this crop as a promising source of polyphenols, a heterogeneous class of secondary metabolites characterized by various healthy properties well-documented in literature. The phenolic fraction, present in the different artichoke plant parts, varies widely in relation to biotic and abiotic factors. Therefore, the present study aimed at evaluating the variation of phenolic content in globe artichoke in relation to biological, technical and environmental factors. Two field-experiments were carried out in Sicily (South Italy in two representative cultivation areas, in order to examine the effects of genotype, head fraction, season conditions, planting density and arrangement on the globe artichoke phenolic concentration. Both the total polyphenols and the individual phenolic compounds detected were notably genotype- dependent. Particularly, the high level of caffeoylquinic acids (chlorogenic acid, among others and apigenin 7- O-glucuronide, reported respectively by “Violetto di Sicilia” and “Romanesco clone C3”, could be used to encourage globe artichoke fresh consumption. Total polyphenols content also resulted more abundant in specific accumulation sites within the inflorescence, such as the floral stem and receptacle, and for most of genotypes it decreased during the second year in response to the different meteorological conditions. Additionally, total polyphenols content significantly and linearly increased as plant density increased from 1.0 to 1.8 plant m-2 and it significantly increased by 13% passing from single to twin rows plant arrangement.

  20. Synbiotic functional drink from Jerusalem artichoke juice fermented by probiotic Lactobacillus plantarum PCS26.

    Science.gov (United States)

    Dimitrovski, Darko; Velickova, Elena; Dimitrovska, Maja; Langerholc, Tomaz; Winkelhausen, Eleonora

    2016-01-01

    A probiotic strain Lactobacillus plantarum PCS26 was used to ferment Jerusalem artichoke juice. Growth kinetics of the bacterial strain was followed during juice fermentation both in flask and in laboratory fermentor. Jerusalem artichoke showed to be an excellent source of nutrients for L. plantarum PCS26 growth. The culture grew very well reaching more than 10(10) cfu/ml in just 12 h. The pH changed from the initial 6.5 to 4.6 at the end of fermentation. The culture hydrolyzed fructooligosaccharides present in the Jerusalem artichoke juice, yielding fructose which was presumably consumed along with the malic acid as energy and carbon source. Lactic acid was the main metabolite produced in concentration of 4.6 g/L. Acetic and succinic acid were also identified. Sensory evaluation of the fermented Jerusalem artichoke juice and its mixtures with blueberry juice showed that the 50/50 % v/v mixture would be very well accepted by the consumers. Above 80 % of the panelists would buy this drink, and over 60 % were willing to pay more for it. Culture survivability in the fermented juices during storage at 4-7 °C was assayed by the Weibullian model. The product shelf-life was extended from 19.70 ± 0.50 days of pure Jerusalem artichoke juice to 35.7 ± 6.4 days of the mixture containing 30 % blueberry juice. PMID:26787997

  1. New method for determining sensory shelf life using fuzzy logic: canned marinated artichoke hearts (Cynara scolymus L.) case

    OpenAIRE

    Víctor Vásquez-Villalobos; Julia Vásquez Angulo; Eduardo Méndez Reyna

    2015-01-01

    The sensory preference (sp) and shelf life of sensory acceptability (SLSA) of canned artichoke hearts were modeled using fuzzy logic (FL) and accelerated testing. The artichoke hearts were marinated in oil of sacha inchi (Plukenetia volubilis), soybean (Glycine max) and olive (Olea europea); and evaluated using a Ranking test with a semi-trained panel, to identify the best preference both for flavor (f) and limpidity (l). We evaluated a global sp through intersection (AND) and union (OR) fuzz...

  2. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats.

    Science.gov (United States)

    Jiménez-Escrig, Antonio; Dragsted, Lars Ove; Daneshvar, Bahram; Pulido, Raquel; Saura-Calixto, Fulgencio

    2003-08-27

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) scavenging, (b) ferric-reducing antioxidant power (FRAP), and (c) inhibition of copper(II)-catalyzed in vitro human low-density lipoprotein (LDL) oxidation. In addition, the present study was performed to investigate the ability of the edible portion of artichoke to alter in vivo antioxidative defense in male rats using selected biomarkers of antioxidant status. One gram (dry matter) had a DPPH(*) activity and a FRAP value in vitro equivalent to those of 29.2 and 62.6 mg of vitamin C and to those of 77.9 and 159 mg of vitamin E, respectively. Artichoke extracts showed good efficiency in the inhibition in vitro of LDL oxidation. Neither ferric-reducing ability nor 2,2'-azinobis(3-ethylbenzothiazolin-6-sulfonate) radical scavenging activity was modified in the plasma of the artichoke group with respect to the control group. Among different antioxidant enzymes measured (superoxide dismutase, gluthatione peroxidase, gluthatione reductase, and catalase) in erythrocytes, only gluthatione peroxidase activity was elevated in the artichoke group compared to the control group. 2-Aminoadipic semialdehyde, a protein oxidation biomarker, was decreased in plasma proteins and hemoglobin in the artichoke-fed group versus the control group. In conclusion, the in vitro protective activity of artichoke was confirmed in a rat model. PMID:12926911

  3. Extraction, degree of polymerization determination and prebiotic effect evaluation of inulin from Jerusalem artichoke.

    Science.gov (United States)

    Li, Wancong; Zhang, Jun; Yu, Chunwei; Li, Qing; Dong, Fang; Wang, Gang; Gu, Guodong; Guo, Zhanyong

    2015-05-01

    The tubers of Jerusalem artichoke are rich of inulin, which makes the plant one of primary inulin resources in China. The aim of this study was to extract inulin from tubers and test the degree of polymerization (DP) 10 days before flowering to 80 days after flowering. The DP of inulin reaches a maximum of 19 at 50 days after flowering. The variation tendencies of inulin content and DP were almost the same, which increase rapidly at the beginning and then decrease gradually at a lower speed. Meanwhile, the effects of inulin on probiotics in yogurt have been evaluated. It indicated that inulin with low DP has higher activities. Experimental data improve the understanding of status change of inulin in whole growth of Jerusalem artichoke tubers in Northeastern China and are instructive to get inulin with different properties.

  4. More than multiple introductions: Multiple taxa contribute to the genesis of the invasive California's wild artichoke thistle

    Institute of Scientific and Technical Information of China (English)

    Janet LEAK-GARCIA; Jodie S.HOLT; Seung-Chul KIM; Lisa MU; José A.MEJ(I)AS; Norman C.ELLSTRAND

    2013-01-01

    The history of some invasive species is so complex that their origins can be difficult to determine.One example of such invasive species is the California invasive known as "wild artichoke thistle" (Cynara cardunculus var.sylvestris),found in natural and disturbed ecosystems.Wild artichoke thistle is a Mediterranean native and the progenitor of two domesticated horticultural taxa,artichoke and cardoon.Different hypotheses regarding the origins of California plants have included introductions by 19th century Italian immigrants and the de-domestication (evolutionary reversion to wild-type morphology) of feral (escaped,free-living) cultivars.Using microsatellite markers,we compared the genetic constitutions of 12 artichoke thistle populations in California with possible progenitor populations:17 Spanish and Italian wild populations and eight different artichoke and cardoon cultivars.Each California population was compared with its putative progenitors using STRUCTURE analysis.Our results suggest that California's artichoke thistle populations are polyphyletic.Surprisingly,two-thirds of California's populations closely matched populations from the Iberian Peninsula.Three populations matched domesticated artichoke.One population appears to have wild and cultivar hybrid ancestry.Alleles specific to Italian populations were found at low frequencies in some California plants,suggesting that Italian wild plants may have been in California,but have left a trivial genetic legacy.Given that the de-domesticated plants in this study appear to be as invasive as the wild taxon,we conclude with a discussion of the role that ferality and de-domestication may have in plant invasions.

  5. Studies on Fermentation of Jerusalem artichoke Juice by Beneficial Lactic Acid Bacteria and the Flavor Compounds of Fermented Jerusalem artichoke Juice%乳酸菌发酵菊芋汁及其风味的研究

    Institute of Scientific and Technical Information of China (English)

    李信; 董英; 程新; 刘崇万

    2012-01-01

    对不同品种和产地的菊芋主要成分进行了测定,并利用乳酸菌对菊芋汁进行发酵,研究了不同乳酸菌在菊芋汁中的生长规律、低温存活性及发酵菊芋汁中主要风味物质。结果表明,4种乳酸菌在菊芋汁中均生长良好,最高活菌数可达到10^9CFU/mL;菊芋汁经乳酸菌发酵后具有良好风味,其风味的差异与发酵菌种有关;发酵菊芋汁中乳酸菌在4℃低温贮藏过程中具有较好的低温存活性,4周后活菌数保持在10^9CFU/mL。菊芋汁适用于开发成新的功能性乳酸菌饮料。%Jerusalem artichoke possesses abundant inulin, high content of potassium and low content of sodium. The main nutritional compositions of Jerusalem artichoke from diverse varieties and regions were analyzed. The main purpose of this study was to investigate the growth of four lactic acid bacteria (Lactobacillus plantarum, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis) on Jerusalem artichoke juice and the production of flavor com- pounds in these bacteria. Growth rule, low-temperature survivability of lactic acid bacteria in Jerusalem artichoke and the major flavor compounds of fermented Jerusalem artichoke juice were considered in this study. In this research it was found that four lactic cultures grew well on the Jerusalem artichoke juice, and the viable cell counts of lactic acid bacteria reached 10^9CFU/mL. Jerusalem artichoke juice that was fermented by beneficial lactic acid bacteria had a good flavor, the main flavor compounds were diverse due to the different species of lactic acid bacteria. Although the lactic cultures of fermented Jerusalem artichoke juice gradually lost their viability during cold storage, the viable cell counts of these lactic acid bacteria still remained at 10^8CFU/mL after 4 weeks of refrigeration at 4℃. The results of this study showed that Jerusalem artichoke juice was a potential substrate to be used for healthy

  6. Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Nan [Agricultural Univ., Qingdao, SD (China). College of Animal Science and Technology; Chinese Academy of Sciences, Qingdao, SD (China). Key Lab. of Biofuels; Yuan, Bo; Wang, Shi-An; Li, Fu-Li [Chinese Academy of Sciences, Qingdao, SD (China). Key Lab. of Biofuels; Sun, Juan [Agricultural Univ., Qingdao, SD (China). College of Animal Science and Technology

    2012-09-15

    Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L{sup -1}) at 40 C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L{sup -1}, which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP. (orig.)

  7. Carbon sequestration and Jerusalem artichoke biomass under nitrogen applications in coastal saline zone in the northern region of Jiangsu, China.

    Science.gov (United States)

    Niu, Li; Manxia, Chen; Xiumei, Gao; Xiaohua, Long; Hongbo, Shao; Zhaopu, Liu; Zed, Rengel

    2016-10-15

    Agriculture is an important source of greenhouse gases, but can also be a significant sink. Nitrogen fertilization is effective in increasing agricultural production and carbon storage. We explored the effects of different rates of nitrogen fertilization on biomass, carbon density, and carbon sequestration in fields under the cultivation of Jerusalem artichoke as well as in soil in a coastal saline zone for two years. Five nitrogen fertilization rates were tested (in guream(-2)): 4 (N1), 8 (N2), 12 (N3), 16 (N4), and 0 (control, CK). The biomass of different organs of Jerusalem artichoke during the growth cycle was significantly higher in N2 than the other treatments. Under different nitrogen treatments, carbon density in organs of Jerusalem artichoke ranged from 336 to 419gCkg(-1). Carbon sequestration in Jerusalem artichoke was higher in treatments with nitrogen fertilization compared to the CK treatment. The highest carbon sequestration was found in the N2 treatment. Soil carbon content was higher in the 0-10cm than 10-20cm layer, with nitrogen fertilization increasing carbon content in both soil layers. The highest soil carbon sequestration was measured in the N2 treatment. Carbon sequestration in both soil and Jerusalem artichoke residue was increased by nitrogen fertilization depending on the rates in the coastal saline zone studied. PMID:27317133

  8. Enzymatic browning and after-cooking darkening of Jerusalem artichoke tubers (Helianthus tuberosus L.)

    DEFF Research Database (Denmark)

    Bach, Vibe; Bennedbæk-Jensen, Sidsel; Clausen, Morten Rahr;

    2013-01-01

    enzymatic browning, but Rema and Draga had higher scores than Mari in after-cooking darkening. Jerusalem artichoke tubers had higher contents of total phenolics, phenolic acids and citric acid in the autumn and low contents in the spring, while it was the opposite for malic acid. None of the chemical......Jerusalem artichoke tubers (Helianthus tuberosus L.) undergo enzymatic browning when peeled or cut, and turn grey after boiling, due to after-cooking darkening reactions between iron and phenolic acids. In an attempt to reveal the components responsible for these discolouration reactions, sensory...... evaluation and instrumental colour measurements were related to contents of total phenolics, phenolic acids, organic acids and iron in three varieties of raw and boiled Jerusalem artichoke tubers harvested in the autumn and the spring. No differences were found between varieties in sensory evaluated...

  9. galaxieEST: addressing EST identity through automated phylogenetic analysis

    Directory of Open Access Journals (Sweden)

    Larsson Karl-Henrik

    2004-07-01

    Full Text Available Abstract Background Research involving expressed sequence tags (ESTs is intricately coupled to the existence of large, well-annotated sequence repositories. Comparatively complete and satisfactory annotated public sequence libraries are, however, available only for a limited range of organisms, rendering the absence of sequences and gene structure information a tangible problem for those working with taxa lacking an EST or genome sequencing project. Paralogous genes belonging to the same gene family but distinguished by derived characteristics are particularly prone to misidentification and erroneous annotation; high but incomplete levels of sequence similarity are typically difficult to interpret and have formed the basis of many unsubstantiated assumptions of orthology. In these cases, a phylogenetic study of the query sequence together with the most similar sequences in the database may be of great value to the identification process. In order to facilitate this laborious procedure, a project to employ automated phylogenetic analysis in the identification of ESTs was initiated. Results galaxieEST is an open source Perl-CGI script package designed to complement traditional similarity-based identification of EST sequences through employment of automated phylogenetic analysis. It uses a series of BLAST runs as a sieve to retrieve nucleotide and protein sequences for inclusion in neighbour joining and parsimony analyses; the output includes the BLAST output, the results of the phylogenetic analyses, and the corresponding multiple alignments. galaxieEST is available as an on-line web service for identification of fungal ESTs and for download / local installation for use with any organism group at http://galaxie.cgb.ki.se/galaxieEST.html. Conclusions By addressing sequence relatedness in addition to similarity, galaxieEST provides an integrative view on EST origin and identity, which may prove particularly useful in cases where similarity searches

  10. Nitrogen and sugar content variability in tubers of Jerusalem artichoke (Helianthus tuberosus

    Directory of Open Access Journals (Sweden)

    Terzić Sreten

    2009-01-01

    Full Text Available Several nutritive values for tubers of 114 Jerusalem artichoke (Helianthus tuberosus populations were evaluated during 2006. The used material is a part of wild sunflower species collection at the Institute of field and vegetable crops and it is situated in Rimski Šancevi, Novi Sad. The samples were analyzed as fresh tubers on 'Venema' automatic laboratory for alpha amino nitrogen, sodium and potassium content. Total sugar content was determined as the brix value on a refractometer. Total nitrogen was determined by the Kjeldahl method on dried samples. Significant variability was found for all analyzed traits. Total nitrogen varied from 0,695 to 2,179% dry weight (mean 1,23%, alpha amino nitrogen content 0,012 to 0,118% fresh weight (m. 0,07%, potassium 0,231 0,452% fresh weight (m. 0,403% and sodium 0,0003 - 0,0143% fresh weight (m. 0,007%. Total sugar content varied from 13,69 - 22,94% fresh weight (m. 19,14%. Alpha amino nitrogen is an essential nutrient for animals so that it's presence in tubers of Jerusalem artichoke as food is positive. The protein content is similar to the one in potato and as such satisfactory for nutrition. The K/Na ratio is high which is useful, because an increased content of potassium in food can positively affect the reduction of Na/K ratio and lower systolic blood pressure by a significant amount in adults with mild hypertension. Inulin makes up to 80% of the total sugar content in the tubers of Jerusalem artichoke, and as a dietary fiber and a fructose polymer it positively influences digestion and sugar blood levels. The obtained results suggest that selection of cultivars and populations with inappropriate nutritive values is possible. Further research is needed to estimate the share of genetic in total variability and to determine whether the selection for new cultivars is justified.

  11. [Effects of hot-NaOH pretreatment on Jerusalem artichoke stalk composition and subsequent enzymatic hydrolysis].

    Science.gov (United States)

    Wang, Qing; Qiu, Jingwen; Li, Yang; Shen, Fei

    2015-10-01

    In order to explore the possibility of Jerusalem artichoke stalk for bioenergy conversion, we analyzed the main composition of whole stalk, pitch, and core of the stalk. Meanwhile, these parts were pretreated with different NaOH concentrations at 121 degrees C. Afterwards, enzymatic hydrolysis was performed to evaluate the pretreatment efficiency. Jerusalem artichoke stalk was characterized by relatively high lignin content (32.0%) compared with traditional crop stalks. The total carbohydrate content was close to that of crop stalks, but with higher cellulose content (40.5%) and lower hemicellulose (19.6%) than those of traditional crop stalks. After pretreatment, the lignin content in the whole stalk, pitch, and core decreased by 13.1%-13.4%, 8.3%-13.5%, and 19.9%-27.2%, respectively, compared with the unpretreated substrates. The hemicellulose content in the whole stalk, pitch, and core decreased 87.8%-96.9%, 87.6%-95.0%, and 74.0%-90.2%, respectively. Correspondingly, the cellulose content in the pretreated whole stalk, pitch, and core increased by 56.5%-60.2%, 52.2%-55.4%, and 62.7%-73.2%, respectively. Moreover, increase of NaOH concentration for pretreatment could improve the enzymatic hydrolysis of the whole stalk and pitch by 2.3-2.6 folds and 10.3-18.5 folds, respectively. The hydrolysis of pretreated stalk core decreased significantly as 2.0 mol/L NaOH was employed, although the increased NaOH concentration can also improve its hydrolysis performance. Based on these results, hot-NaOH can be regarded as an option for Jerusalem artichoke stalk pretreatment. Increasing NaOH concentration was beneficial to hemicellulose and lignin removal, and consequently improved sugar conversion. However, the potential decrease of sugar conversion of the pretreated core by higher NaOH concentration suggested further optimization on the pretreatment conditions should be performed. PMID:26964335

  12. CitEST libraries

    Directory of Open Access Journals (Sweden)

    Maria Luísa P. Natividade Targon

    2007-01-01

    Full Text Available In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia. In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5’ end, generating 242,790 valid reads of citrus. A total of 9,504 sequences were produced in the shotgun library and the valid reads were assembled using CAP3. In this procedure, we obtained 1,131 contigs and 4,083 singletons. A total of 19,200 cDNA clones from P. parasitica were sequenced, resulting in 16,400 valid reads. The number of ESTs generated in this project is, to our knowledge, the largest citrus sequence database in the world.

  13. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L. in a Biorefinery Concept

    Directory of Open Access Journals (Sweden)

    Eva Johansson

    2015-04-01

    Full Text Available Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L. is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals as a single product was too expensive to be competitive with petrochemically produced sugars. Therefore, production of several products from the same crop is a must. Additional products are protein based ones from tubers and leaves and biogas from residues, although both are of low value and amount. High bioactive activity was found in the young leaves of the crop, and the sesquiterpene lactones are of specific interest, as other compounds from this group have shown inhibitory effects on several human diseases. Thus, future focus should be on understanding the usefulness of small molecules, to develop methods for their extraction and purification and to further develop sustainable and viable methods for the production of platform chemicals.

  14. Screening of Jerusalem artichoke varieties for bio-ethanol production in Portugal

    Energy Technology Data Exchange (ETDEWEB)

    Passarinho, P.C.; Oliveira, A.C.; Rosa, M.F. [INETI, Departamento de Energias Renovaveis, Estrada do Paco do Lumiar, Ed. G, 1649-038, Lisboa (Portugal)

    2008-07-01

    The aim of this work was the evaluation of the potential of 9 Jerusalem artichoke varieties for the sustainable production of bio-ethanol in Portugal. The tubers, which are the part of the plant with higher sugar content, were harvested at different stages of development (29 to 55 weeks), and crashed for juice extraction. The two phases obtained were characterized in terms of total sugars, protein, ash and dry matter. The ethanol productivity of the different J. artichoke varieties was then evaluated fermenting juice or mixtures of juice and pulp aqueous extract with a strain of Kluyveromyces marxianus, a yeast able to hydrolyze and ferment inulin polymers. The chamical characteristic more dependable on the harvest period was the amount of total sugars in the tubers. Juices, obtained until 48 weeks development, contained 173 - 235 g/L of total sugars while juices from the last harvest presented markedly lower sugar contents, indicating crop degradation or sugar migration to the soil. Regarding the fermentative process, ethanol yields ranged from 0.3 to 0.5 g/g. The main conclusion of this work indicates C13 variety as the best. Although bearing a lower sugar concentration in tubers, the substantially higher agricultural productivities (kg/m2) after 8 months growing allowed to estimate productions higher than 10 000 L/ha.

  15. Effect of Lactobacillus paracasei Culture Filtrates and Artichoke Polyphenols on Cytokine Production by Dendritic Cells

    Science.gov (United States)

    Sisto, Angelo; Luongo, Diomira; Treppiccione, Lucia; De Bellis, Palmira; Di Venere, Donato; Lavermicocca, Paola; Rossi, Mauro

    2016-01-01

    The most recent trend in research on probiotic bacteria aims at the exploitation of bioactive bacterial compounds that are responsible for health-promoting effects and suitable for medical applications. Therefore, the main purpose of this study was to ascertain if the immunomodulatory effects of L. paracasei strains on dendritic cells (DCs) were caused by bacterial metabolites released in the culture medium. For that reason, bacterial strains were grown in two media generally used for the culture of DCs, and the effects of culture filtrates on the maturation of DCs and cytokine production were evaluated. Moreover, to reveal potential synergistic effects on the immunomodulation of DCs, an artichoke phenolic extract (APE) was added to the media before bacterial growth. The experiments pointed out an interesting anti-inflammatory activity of a culture filtrate obtained after growing a probiotic L. paracasei strain in one of the media supplemented with APE. Therefore, this culture filtrate—which combines the anti-inflammatory activity and the other well-known health-promoting properties of artichoke phenolic compounds—could represent the basis for future particular exploitations. PMID:27754398

  16. Protective Role of Ca Against NaCl Toxicity in Jerusalem Artichoke by Up-Regulation of Antioxidant Enzymes

    Institute of Scientific and Technical Information of China (English)

    XUE Yan-Feng; LIU Ling; LIU Zhao-Pu; S. K.MEHTA; ZHAO Geng-Mao

    2008-01-01

    The ameliorative effect of external Ca2+ on Jerusalem artichoke (Helianthus tuberosus L.) under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with or without 10 mol L-1 CaCl2, 150 mmol L-1 NaCl, and/or 5 mmol L-1 ethylene-bis(oxyethylenenitrilo)-tetraacetic acid (EGTA) for five days. Exposure to NaCl (150 mmol L-1) decreased growth, leaf chlorophyll content, and photosynthetic rate of Jerusalem artichoke seedlings. NaCl treatment showed 59% and 37% higher lipid peroxidation and electrolyte leakage, respectively, than the control. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were decreased by NaCl, indicating an impeded antioxidant defense mechanism of Jerusalem artichoke grown under salt stress. Addition of 10 mmol L-1 CaCl2 to the salt solutions significantly decreased the damaging effect of NaCl on growth and chlorophyll content and simultaneously restored the rate of photosynthesis almost to the level of the control. Ca2+ addition decreased the leaf malondialdehyde (MDA) content and electrolyte leakage from NaCl-treated seedlings by 47% and 24%, respectively, and significantly improved the activities of SOD, POD, and CAT in NaCl-treated plants. Addition of ECTA, a specific chelator of Ca2+, decreased the growth, chlorophyll content, and photosynthesis, and increased level of MDA and electrolyte leakage from NaCl-treated plants and from the control plants. ECTA addition to the growth medium also repressed the activities of SOD, POD, and CAT in NaCl-treated and control seedlings. External Ca2+ might protect Jerusalem artichoke against NaCl stress by up-regulating the activities of antioxidant enzymes and thereby decreasing the oxidative stress.

  17. EST-PAC a web package for EST annotation and protein sequence prediction

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    Strahm Yvan

    2006-10-01

    Full Text Available Abstract With the decreasing cost of DNA sequencing technology and the vast diversity of biological resources, researchers increasingly face the basic challenge of annotating a larger number of expressed sequences tags (EST from a variety of species. This typically consists of a series of repetitive tasks, which should be automated and easy to use. The results of these annotation tasks need to be stored and organized in a consistent way. All these operations should be self-installing, platform independent, easy to customize and amenable to using distributed bioinformatics resources available on the Internet. In order to address these issues, we present EST-PAC a web oriented multi-platform software package for expressed sequences tag (EST annotation. EST-PAC provides a solution for the administration of EST and protein sequence annotations accessible through a web interface. Three aspects of EST annotation are automated: 1 searching local or remote biological databases for sequence similarities using Blast services, 2 predicting protein coding sequence from EST data and, 3 annotating predicted protein sequences with functional domain predictions. In practice, EST-PAC integrates the BLASTALL suite, EST-Scan2 and HMMER in a relational database system accessible through a simple web interface. EST-PAC also takes advantage of the relational database to allow consistent storage, powerful queries of results and, management of the annotation process. The system allows users to customize annotation strategies and provides an open-source data-management environment for research and education in bioinformatics.

  18. Potential of Jerusalem artichoke (Helianthus tuberosus L.) as a biorefinery crop

    DEFF Research Database (Denmark)

    Gunnarsson, Ingólfur Bragi; Svensson, S.-E.; Johansson, E.;

    2014-01-01

    The utilization of Jerusalem artichoke in a biorefinery context was not investigated so far. Therefore the aim of this study was to evaluate the potential of this plant as feedstock for production of bioethanol, protein and inulin. We investigated the biomass productivity and chemical composition...... biomass productivity was 88% higher in September than in December. Fresh tuber biomass productivity showed large variations between harvests, where the maximum average productivity in December was 3.4 times higher than in September. Inulin content in dry tubers was between 76 and 85% making the plant...... an excellent crop, for e.g. inulin extraction, production of high fructose syrup or fermentations. Less mature plants were shown to have degree of polymerization (DP) up to 14, which makes biomass useful as dietary fibre, while the inulin DP in tubers harvested later became as low as 6, showing lower potential...

  19. Syneresis, rheological characteristic and sensory consistency of the artichoke sauce (Cynara scolymus L.

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    Francisco Mora Barandiarán

    2013-09-01

    Full Text Available The effect of the concentration of three hydrocolloids: CMC, guar gum and xanthan gum on the syneresis, rheological features and sensory consistency of the artichoke sauce (Cynara scolymus L. Imperial Star variety was studied. The syneresis was determined by the water loss by centrifugation, the rheological features of the artichoke sauce was determined using a Brookfield RVDV – III model rheometer and finally, the sensory consistency was determined by measuring the degree of satisfaction with hedonic scale of nine points. A simplex lattice design blends with centroid expanded under the response surface methodology was applied to establish the effect of the concentration of hydrocolloid on syneresis, rheological features and sensory consistency. In all treatments, the apparent viscosity decreased with shear rate demonstrating a “non Newtonian” behavior of “general plastic” type with an “n” value less than 1 and an initial shear stress. The flow behavior index “n” was in the range of 0.0856 and 0.3131 (n < 1 and the consistency index “k” in the range of 84.55 y 167.80 Pa.sn , the initial shear stress was in the range of 9,10 y 13,51 Pa and consistency sensory presented score of “like” to “like slightly”. Finally, the hydrocolloid mixture was optimized over the area of feasible formulation. With the optimal mixture, corresponding to 0.28% CMC, 0.13% guar gum and 0.59% xanthan gum is expected to obtain a 0.089% of syneresis and a rating of 6 in terms of sensory consistency.

  20. Jerusalem artichoke (Helianthus tuberosus L. productivity in different Italian growing areas: a modelling approach

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    Mario Baldini

    2011-06-01

    Full Text Available Jerusalem artichoke is considered one of the more interesting crops for inulin production. It has been used to date for the production of low calorie sweetening syrups, dietetic food enriched with fibre, medicines and cosmetics, while more recently, interesting prospects have been opening up for energy uses. The main aspects influencing its adaptability to different pedo-climatic conditions and cropping systems were analysed by implementing CSS (Cropping System Simulator, a simulation model describing crop biomass production for this specific crop. Growth analysis experimental data of plant dry matter accumulation, obtained over two years of trials (1999-2000 in different Italian growing areas (Udine, Bologna, Bari under irrigated and rain-fed conditions, were used for the parameterisation and calibration of the model. The biomass accumulation observed and simulated under rain-fed and irrigated conditions in the different growing areas is reported for the different plant organs, with good correspondence shown between simulated and measured values as reported by the statistical indices for the model calibration, particularly for biomass of tubers and leaves. The model studied, despite a simplified description of some processes, proves to represent the maximum biomass yield of Jerusalem artichoke satisfactorily, with an adequate response to the main environmental factors causing yield and biomass production variation among the years and locations. However, further model improvements are necessary in order to better represent the relationship between phenology and translocation of the assimilates between stalk and tuber during the development phases of the plant, suggesting shorter survey intervals over this growing phase.

  1. Jerusalem artichoke as a platform for inulin, ethanol and feed production in Canada

    Energy Technology Data Exchange (ETDEWEB)

    Anyia, A.O.; Mostafa, H.; Melnichuk, R.; Slaski, J.J. [Alberta Research Council, Vegreville, AB (Canada). Bioresource Technologies Unit

    2009-07-01

    The Alberta Research Council (ARC) is developing an extraction and fermentation process for making ethanol from Jerusalem artichoke (JA). In particular, ARC has collaborated with Olds College in developing an extraction process and an engineering process for the commercial production of inulin, ethanol, polymers and animal feed from JA tubers. Fresh JA tubers contain about 20 per cent of water soluble carbohydrates, which occur primarily in the form of inulin. Several health promoting benefits are associated with intake of inulin. High volumes of dry residual aerial biomass following tuber harvest contain 40 to 50 per cent water soluble carbohydrates that are fermentable to ethanol. Some studies have shown that under optimal climatic conditions, JA can yield more ethanol per ha than sugarcane. ARC has the exclusive North American rights to several high yielding JA cultivars. Jerusalem artichoke is not a designated food crop and has a high biomass yield for soluble sugars. This perennial crop forms tubers, has a deep root system that can be adapted to marginal lands. ARC's research involves a seed to final product technology development approach that includes new variety development, agronomy and processing. ARC applied a hot water extraction technique along with a low liquid to JA stalk ratio to achieve more than 40 per cent total water soluble carbohydrates per gram of biomass that are fermentable to ethanol without the need for weak acid or enzymatic hydrolysis. A 400 hectare plantation of JA in Alberta could produce about 1,500 tonnes of inulin and 1.5 million liters of ethanol per year in a pilot scale bio-refining plant. An economic and market analysis showed that capital investments in an inulin production plant in Alberta will be a profitable venture. ARC has estimated a 5 year Internal Rate of Return (IRR) to range from 10 to 30 per cent and payback period of 4 to 5 years depending on plant location and value of by-products. tabs., figs.

  2. Artichoke and milk thistle pills and syrups as sources of phenolic compounds with antimicrobial activity.

    Science.gov (United States)

    Pereira, Carla; Barros, Lillian; José Alves, Maria; Santos-Buelga, Celestino; Ferreira, Isabel C F R

    2016-07-13

    Dietary supplements based on hepatoprotective plants have been increasingly used in the prevention of liver injuries. In the present work, the aim was to study the phenolic profile and possibly relate it to the in vitro antimicrobial activity of two different formulations (pills and syrups) of artichoke and milk thistle, the antioxidant and anti-hepatocellular carcinoma activities of which were previously reported by our research group. The phenolic profiles were obtained by HPLC-DAD-ESI/MS, and the antimicrobial activity evaluation was performed with the clinical isolates of multiresistant bacteria (Escherichia coli, extended spectrum β-lactamases (ESBL) producing Escherichia coli, Proteus mirabilis, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa). Artichoke syrup revealed the presence of vanillic acid and luteolin-7-O-glucoside while the pills possessed higher concentrations of 4-O-caffeoylquinic, 5-O-caffeoylquinic and 1,3-O-dicaffeoylquinic acids, this latest being able to inhibit the growth of MRSA. Regarding milk thistle formulations, the syrup presented isorhamnetin-O-deoxyhexoside-O-dihexoside, isorhamnetin-O-deoxyhexoside-O-hexoside and isorhamnetin-3-O-rutinoside as the major phenolic constituents whereas the pills were richer in taxifolin, silymarin derivatives and hydroxylated silibinin; the syrup revealed antimicrobial activity against all the studied bacteria with the exception of Proteus mirabilis whereas the pills revealed activity against ESBL producing Escherichia coli. Overall, all of the studied formulations revealed to be a good source of phenolic compounds, among which milk thistle syrup presented the highest variety and concentration of flavonoids, which is possibly related to its strongest antimicrobial activity. PMID:27273551

  3. Genetic mapping and identification of QTL for earliness in the globe artichoke/cultivated cardoon complex

    Directory of Open Access Journals (Sweden)

    Portis Ezio

    2012-05-01

    Full Text Available Abstract Background The Asteraceae species Cynara cardunculus (2n = 2x = 34 includes the two fully cross-compatible domesticated taxa globe artichoke (var. scolymus L. and cultivated cardoon (var. altilis DC. As both are out-pollinators and suffer from marked inbreeding depression, linkage analysis has focussed on the use of a two way pseudo-test cross approach. Results A set of 172 microsatellite (SSR loci derived from expressed sequence tag DNA sequence were integrated into the reference C. cardunculus genetic maps, based on segregation among the F1 progeny of a cross between a globe artichoke and a cultivated cardoon. The resulting maps each detected 17 major linkage groups, corresponding to the species’ haploid chromosome number. A consensus map based on 66 co-dominant shared loci (64 SSRs and two SNPs assembled 694 loci, with a mean inter-marker spacing of 2.5 cM. When the maps were used to elucidate the pattern of inheritance of head production earliness, a key commercial trait, seven regions were shown to harbour relevant quantitative trait loci (QTL. Together, these QTL accounted for up to 74% of the overall phenotypic variance. Conclusion The newly developed consensus as well as the parental genetic maps can accelerate the process of tagging and eventually isolating the genes underlying earliness in both the domesticated C. cardunculus forms. The largest single effect mapped to the same linkage group in each parental maps, and explained about one half of the phenotypic variance, thus representing a good candidate for marker assisted selection.

  4. Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats

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    Crevar-Sakač Milkica

    2016-01-01

    Full Text Available Backgroung/Aim. Since combining conventional drugs with herbal medicinal products is in current research focus and possible of great interest as therapy improvement way, the aim of this study was to determine the effects of wellestablished antiatherosclerotic drug atorvastatin (CAS number 134523-00-5 and commercially available artichoke leaf tincture (ALTINC, used as combined therapy, as well as to compare effects of these two treatments separately. Methods. Experimental animals were divided into five groups: the group I (the control group of rats fed with standard diet during 11 weeks, and the remaining 4 groups of rats (II, III, IV and V fed with standard diet during the first week and then with hypercholesterolemic diet during the next 10 weeks. The group II of rats were left without treatment, while in the groups III, IV and V were rats treated per os with atorvastatin (1.15 mg/kg body wright − b.w., ALTINC (0.1 mL/kg b.w. and their combination in same doses, respectively, for the last six weeks. Results. The cholesterol rich diet led to pronounced hyperlipidemia which could not be overcame with the therapy. However, the therapy showed positive effects on abdominal aorta wall thickness and parameters of oxidative stress (malondialdehyde − MDA, proxidative-antioxidative balance − PAB and antioxidative protection (reduced glutathione − GSH, paraoxanase 1 − PON1, superoxide dismutase − SODA SH groups, especially ALTINC was successful in oxidative status improvement. Conclusion. Separate treatments comparison showed that artichoke leaf tincture is very potent antioxidant with beneficial effects in early stages of atherosclerosis. Since atorvastatin and constituents of ALTINC probably have different mechanisms of action, simultaneous use of both therapies could be beneficial but should be further investigated since our results showed that ALTINC is less effective when used in combination with atorvastatin. [Projekat Ministarstva nauke

  5. Influence of cooking conditions on organoleptic and health-related properties of artichokes, green beans, broccoli and carrots.

    Science.gov (United States)

    Guillén, Sofía; Mir-Bel, Jorge; Oria, Rosa; Salvador, María L

    2017-02-15

    Colour, pigments, total phenolic content and antioxidant activity were investigated in artichokes, green beans, broccoli and carrots cooked under different conditions. Domestic induction hobs with temperature control were used to evaluate the effect of boiling, sous-vide cooking and water immersion cooking at temperatures below 100°C on the properties of each vegetable. Sous-vide cooking preserved chlorophyll, carotenoids, phenolic content and antioxidant activity to a greater extent than boiling for all of the vegetables tested and retained colour better, as determined by a(∗). A reduction of only 10-15°C in the cooking temperature was enough to improve the properties of the samples cooked by water immersion, except for green beans. Artichokes and carrots suffered pronounced losses of antioxidant activity during boiling (17.0 and 9.2% retention, respectively), but the stability of this parameter significantly increased with sous-vide cooking (84.9 and 55.3% retention, respectively).

  6. Cellulosic bioethanol production from Jerusalem artichoke (Helianthus tuberosus L.) using hydrogen peroxide-acetic acid (HPAC) pretreatment.

    Science.gov (United States)

    Song, Younho; Wi, Seung Gon; Kim, Ho Myeong; Bae, Hyeun-Jong

    2016-08-01

    Jerusalem artichoke (JA) is recognized as a suitable candidate biomass crop for bioethanol production because it has a rapid growth rate and high biomass productivity. In this study, hydrogen peroxide-acetic acid (HPAC) pretreatment was used to enhance the enzymatic hydrolysis and to effectively remove the lignin of JA. With optimized enzyme doses, synergy was observed from the combination of three different enzymes (RUT-C30, pectinase, and xylanase) which provided a conversion rate was approximately 30% higher than the rate with from treatment with RUT-C30 alone. Fermentation of the JA hydrolyzates by Saccharomyces cerevisiae produced a fermentation yield of approximately 84%. Therefore, Jerusalem artichoke has potential as a bioenergy crop for bioethanol production. PMID:27115748

  7. Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster

    Science.gov (United States)

    Jacociunas, Laura Vicedo; Dihl, Rafael Rodrigues; Lehmann, Mauricio; de Barros Falcão Ferraz, Alexandre; Richter, Marc François; da Silva, Juliana; de Andrade, Heloísa Helena Rodrigues

    2014-01-01

    The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate – EMS and mitomycin C – MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Neither the mutagenicity nor the recombinogenicity of BLM or MMC was modified by co- or post-treatment with BHE or LE. In contrast, co-treatment with BHE significantly enhanced the EMS-induced genotoxicity involving mutagenic and/or recombinant events. Co-treatment with LE did not alter the genotoxicity of EMS whereas post-treatment with the highest dose of LE significantly increased this genotoxicity. This enhancement included a synergistic increase restricted to somatic recombination. These results show that artichoke extracts promote homologous recombination in proliferative cells of D. melanogaster. PMID:24688296

  8. The content of protein and of amino acids in Jerusalem artichoke tubers (Helianthus tuberosus L. of red variety Rote Zonenkugel

    Directory of Open Access Journals (Sweden)

    Ewa Cieślik

    2011-12-01

    Full Text Available   Introduction. Jerusalem artichoke (Helianthus tuberosus L. is grown primarily for its edible tubers, which were first cultivated by native Americans before the arrival of the Europeans. Unlike most tubers, but in common with other members of the Asteraceae, the tubers store fructans instead of starch. Fructans are non-digestible carbohydrates considered functional food ingredients because they affect body processes in ways that result in better health and in many diseases prevention. However, the Jerusalem artichoke deserves attention not only because of the content of fructans, recent studies also indicate a high protein content, including essential amino acids. Material and methods. The aim of the work was to establish the content of protein and amino acids in Jerusalem artichoke tubers (Helianthus tuberosus L. of red variety – Rote Zonenkugel. The content of protein was estimated by Dumas method. The amino acids composition was analysed with ion-change chromatography with postcolumn derivatisation and detection of ninhydryn reaction with automatic amino acids analyser. Results. The assessed liophylisate was characterised by high protein content (6.36% in comparison to chicory (which is the main industrial source of fructans and to commonly consumed potatoes. There was shown a few times higher content of essential amino acids (also of methionine in comparison to chicory and potato. The examined essential amino acids were present in very advantagenous proportions. Conclusions. In Jerusalem artichoke tubers of Rote Zonenkugel variety of the high content of protein was established in comparison to other plant sources. The high content was found of amino acids with special stress on essential amino acids (esp. sulphur ones.  

  9. Cloning and functional characterization of two abiotic stress-responsive Jerusalem artichoke (Helianthus tuberosus) fructan 1-exohydrolases (1-FEHs).

    Science.gov (United States)

    Xu, Huanhuan; Liang, Mingxiang; Xu, Li; Li, Hui; Zhang, Xi; Kang, Jian; Zhao, Qingxin; Zhao, Haiyan

    2015-01-01

    Two fructan hydrolases were previously reported to exist in Jerusalem artichoke (Helianthus tuberosus) and one native fructan-β-fructosidase (1-FEH) was purified to homogeneity by SDS-PAGE, but no corresponding cDNA was cloned. Here, we cloned two full-length 1-FEH cDNA sequences from Jerusalem artichoke, named Ht1-FEH I and Ht1-FEH II, which showed high levels of identity with chicory 1-FEH I and 1-FEH II. Functional characterization of the corresponding recombinant proteins in Pichia pastoris X-33 demonstrated that both Ht1-FEHs had high levels of hydrolase activity towards β(2,1)-linked fructans, but low or no activity towards β(2,6)-linked levan and sucrose. Like other plant FEHs, the activities of the recombinant Ht1-FEHs were greatly inhibited by sucrose. Real-time quantitative PCR analysis showed that Ht1-FEH I transcripts accumulated to high levels in the developing leaves and stems of artichoke, whereas the expression levels of Ht1-FEH II increased in tubers during tuber sprouting, which implies that the two Ht1-FEHs play different roles. The levels of both Ht1-FEH I and II transcript were significantly increased in the stems of NaCl-treated plants. NaCl treatment also induced transcription of both Ht1-FEHs in the tubers, while PEG treatments slightly inhibited the expression of Ht1-FEH II in tubers. Analysis of sugar-metabolizing enzyme activities and carbohydrate concentration via HPLC showed that the enzyme activities of 1-FEHs were increased but the fructose content was decreased under NaCl and PEG treatments. Given that FEH hydrolyzes fructan to yield Fru, we discuss possible explanations for the inconsistency between 1-FEH activity and fructan dynamics in artichokes subjected to abiotic stress. PMID:25522837

  10. Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry

    Science.gov (United States)

    Helmi, Zead; Al Azzam, Khaldun Mohammad; Tsymbalista, Yuliya; Ghazleh, Refat Abo; Shaibah, Hassan; Aboul-Enein, Hassan

    2014-01-01

    Purpose: To investigate, for the first time, the chemical composition of essential oil of the tubers and leaves of Jerusalem artichoke (Helianthus tuberosus L.), a species of sunflower native to eastern North America, growing in Ukraine. Methods: A hydrodistillation apparatus was used for the extraction of volatile components and then it was analysed by gas chromatography equipped with a split-splitless injector (split ratio, 1:50) and flame ionization detector (FID). The oil was analyzed under linear temperature programming applied at 4°C/min from 50°C - 340°C. Temperatures of the injector and FID detector were maintained at 280°C and 300°C, respectively. The chemical analysis of the oil was carried out using gas chromatography coupled to mass spectrometry (GC-MS), to determine the chemical composition of the volatile fraction. Results: The essential oils content ranged from 0.00019 to 0.03486 and 0.00011 to 0.00205 (g/100g), in leaves and tubers, respectively. The qualitative and quantitative analysis led to the identification of 17 components in both species samples. The major component found in leaves and tubers was (-)-β-bisabolene with 70.7% and 63.1%, respectively. Conclusion: Essential oil profile of Jerusalem artichoke species showed significant differences between leaves and tubers species. Additionally, the leaves of Jerusalem artichoke are a promising source of natural β-bisabolene. PMID:25671184

  11. Conte nt of nutritive components, dietary fi bre and energy value of artichoke depending on the variety

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    Iwona Mentel

    2012-06-01

    Full Text Available Background. The artichoke (Cynara scolymus L. is a perennial plant belonging to Asteraceae family. It is less popular vegetable in Poland but very valuable from nutritive and medicinal points of view plant. Material and methods. The experiments were conducted in 2008 and 2009 on fi ve artichoke varieties: ‘Deutscher Hybrid’, ‘Emerald F1’, Green Globe’, ‘Gros Camus de Bretague’ and ‘Kerlouan’, grew in Poland, France and Austria. The experimental material was assessed for: dry mass, protein, ash, vitamin C and dietary fi ber contents and some mineral components (calcium, magnesium, phosphorus, potassium, sodium, iron, zinc levels. Also the energy value and total carbohydrates content were calculated in the vegetable. Results. Among assessed varieties, the highest contents of such components as: dry mass (total solids, carbohydrates, ash, vitamin C, sodium, potassium, magnesium, iron were found in ‘Gros Camus de Bretague’. The hearts of that artichoke had also higher energy value than others. Whereas the lowest ash, phosphorus, sodium, potassium and magnesium were noticed in ‘Kerlouan’ variety. But the highest values of dietary fi ber was observed in that variety. Conclusions. Basing on performed assessments the differences in nutritive components and dietary fi ber, between analysed vegetable varieties were found.

  12. Soil Properties and Yield of Jerusalem Artichoke (Helianthus tuberosus L.) with Seawater Irrigation in North China Plain

    Institute of Scientific and Technical Information of China (English)

    ZHAO Geng-Mao; LIU Zhao-Pu; CHEN Ming-Da; GUO Shi-Wei

    2008-01-01

    Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of the North China Plain.Jerusalem artichoke (Helianthus tuberosus L.) grown in a field experiment was irrigated with seawater diluted with freshwater from 2001 to 2003 to determine the feasibility of seawater irrigation in the Laizhou area.For treatments of CK (non-irrigation) along with seawater concentrations of 25%,50%,and 75%,total dissolved solid (TDS) in the non-irrigated soil significantly increased (P ≤0.05) in both 2002 and 2003 and was 1.3 times higher in 2003 than in 2001.In the 25%and 50% seawater concentration treatments,TDS in 2001 was significantly greater (P ≤ 0.05) than CK;however,TDS in these two treatments decreased by 34.9% and 40.1%,respectively,in 2003 compared with 2001.The sodium adsorption ratio (SAR) remained below 10 mmol1/2 L-1/2,indicating that alkalization was low with seawater irrigation.In 2001 and 2002,compared to CK and the irrigation treatment with 75% seawater,irrigation with 25% and 50% seawater increased the yields of Jerusalem artichoke,This meant that Jerusalem artichoke could be safely grown in salt-affected land of Laizhou area with 25% and 50% seawater irrigation.

  13. Chicory and Jerusalem artichoke productivity in different areas of Italy, in relation to water availability and time of harvest

    Directory of Open Access Journals (Sweden)

    Giuseppe De Mastro

    2006-06-01

    Full Text Available Inulin is an important polysaccharide synthesised by different crops, which, in the EU has been included in the system of sugar quotas since 1994. Currently, one of the major problems of the agro-industry is the need to extend the length of the sugar crop harvest season. It was therefore decided, also in relation to the increased demand for inulin, to study the two main inulin producing crops in Italy (chicory and Jerusalem artichoke, to verify yield and quality potential and stability in relation to some important agronomic factors such as irrigation and time of harvest. The work was conducted in 1999 and 2000 in four areas of Italy (Udine, Rovigo, Bologna and Bari. The effects evaluated were time of harvest (3 for chicory and 2 for Jerusalem artichoke and irrigation system (evapotranspiration replacement and dry regime, with irrigation applied only when strictly necessary on the production of storage organs, sugars and inulin in the two crops. The highest chicory root yield was in Bologna, with an average production of 65.6 t ha-1 (fresh weight, compared to Rovigo (54.4 t ha-1, Bari (46.5 t ha-1 and Udine (38.7 t ha-1. For final tuber yield in Jerusalem artichoke, Bari was the most productive environment with an average of 80 t ha-1, followed by Bologna (61 t ha-1 and Udine (55.5 t ha-1. However, when this crop is whole-plant harvested (stalks and tubers at pre-flowering, Bologna, with high stalk yields (58.7 t ha-1 appeared to be the most suitable environment. This type of harvesting was also shown to be more productive in terms of sugar and inulin yield. The total sugar content in the different organs analysed (roots, stalk and tubers was always higher in Udine compared to Bologna, for both crops. Lastly, the length of the inulin chain (average degree of polymerisation [DP] diminishes with the delaying of the harvest in both crops. The Bologna area had the highest potential in terms of chicory root production, while for the tubers yield of

  14. Relational databases

    CERN Document Server

    Bell, D A

    1986-01-01

    Relational Databases explores the major advances in relational databases and provides a balanced analysis of the state of the art in relational databases. Topics covered include capture and analysis of data placement requirements; distributed relational database systems; data dependency manipulation in database schemata; and relational database support for computer graphics and computer aided design. This book is divided into three sections and begins with an overview of the theory and practice of distributed systems, using the example of INGRES from Relational Technology as illustration. The

  15. Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid

    Directory of Open Access Journals (Sweden)

    Tanupat Mornkham

    2013-04-01

    Full Text Available Jerusalem artichoke (Helianthus tuberosus L. is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicability of the improved method in other plant species. Five RNA extraction methods were evaluated on Jerusalem artichoke seeds and two were modified. One modified method with the significant improvement was applied to assay seeds of diverse Jerusalem artichoke accessions, sunflower, rice, maize, peanut and marigold. The effectiveness of the improved method to extract total RNA from seeds was assessed using qPCR analysis of four selected genes. The improved method of Ma and Yang (2011 yielded a maximum RNA solubility and removed most interfering substances. The improved protocol generated 29 to 41 µg RNA/30 mg fresh weight. An A260/A280 ratio of 1.79 to 2.22 showed their RNA purity. Extracted RNA was effective for downstream applications such as first-stranded cDNA synthesis, cDNA cloning and qPCR. The improved method was also effective to extract total RNA from seeds of sunflower, rice, maize and peanut that are rich in polyphenols, lipids and polysaccharides.

  16. Germinação in vitro de sementes de alcachofra In vitro artichoke seed germination

    Directory of Open Access Journals (Sweden)

    Cassieli F de Moraes

    2010-03-01

    Full Text Available A baixa taxa de multiplicação e alta de contaminação dos explantes são algumas das dificuldades na micropropagação da alcachofra. A germinação de sementes in vitro pode ser uma alternativa de obtenção de explantes sadios para estabelecimento de futuros cultivos in vitro. O trabalho desenvolvido no Laboratório de Biotecnologia Vegetal da UPF-FAMV teve por objetivo avaliar a germinação in vitro de sementes de alcachofra cv. Nobre, em três experimentos, testando concentrações de cloro ativo na assepsia das sementes; tratamentos do tegumento (mantido intacto, com cortes laterais ou eliminação; condições de luminosidade (claro ou escuro; e dois meios de cultura [meio MS, com concentração de sais reduzida à metade (M1 e meio MS completo(M2]. Em ambos foram adicionados 30 g L-1 de sacarose e 7 g L-1 de ágar, sendo o pH ajustado para 5,6 ± 0,1 com NaOH. Os cultivos foram realizados em câmara de crescimento. A obtenção de plântulas sadias de alcachofra em curto espaço de tempo (sete dias, para utilização como fonte de explantes é viável a partir da germinação in vitro de sementes sem o tegumento (77,5% de germinação, utilizando os meios de cultura M1 ou M2 e câmara de crescimento desprovida de luz. Nestas condições, a assepsia das sementes pode ser realizada com álcool 70% por 30 min e posterior imersão em solução contendo 2% de cloro ativo por dez minutos, antes da remoção do tegumento.Low multiplication rates and high contamination in the explants are some of the difficulties in artichoke micropropagation. In vitro seed germination may be an alternative to obtain healthy explants for use in future in vitro cultivation. This project developed at the laboratory of Universidade de Passo Fundo was established to evaluate cv. 'Nobre' artichoke seeds in vitro germination. In three experiments, active chloride concentrations on seed aseptic technique; tegument treatment (kept intact, with side cuts and

  17. Annotation of Ehux ESTs

    Energy Technology Data Exchange (ETDEWEB)

    Kuo, Alan; Grigoriev, Igor

    2009-06-12

    22 percent ESTs do no align with scaffolds. EST Pipeleine assembles 17126 consensi from the noaligned ESTs. Annotation Pipeline predicts 8564 ORFS on the consensi. Domain analysis of ORFs reveals missing genes. Cluster analysis reveals missing genes. Expression analysis reveals potential strain specific genes.

  18. Biofuel Database

    Science.gov (United States)

    Biofuel Database (Web, free access)   This database brings together structural, biological, and thermodynamic data for enzymes that are either in current use or are being considered for use in the production of biofuels.

  19. Community Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This excel spreadsheet is the result of merging at the port level of several of the in-house fisheries databases in combination with other demographic databases...

  20. Database Administrator

    Science.gov (United States)

    Moore, Pam

    2010-01-01

    The Internet and electronic commerce (e-commerce) generate lots of data. Data must be stored, organized, and managed. Database administrators, or DBAs, work with database software to find ways to do this. They identify user needs, set up computer databases, and test systems. They ensure that systems perform as they should and add people to the…

  1. Research Progress of Exploitation and Utilization of Jerusalem artichoke Resources%菊芋资源开发及利用研究进展

    Institute of Scientific and Technical Information of China (English)

    郭洪涛; 郭衍银

    2011-01-01

    本文在阐述菊芋价值、菊糖特性的基础上,对菊糖提取方法、菊芋制品研究现状进行了综述,同时就菊芋资源开发和利用中存在问题和发展方向提出了几点看法.%On the basis of elaborating the value of Jerusalem artichoke and the properties of inulin, the research situation of inulin extraction methods and Jerusalem artichoke products was summarized in this paper. Meanwhile, some suggestions were put forward for the present problems and developing direction in exploitation and utilization of Jerusalem artichoke.

  2. Preparation of Inulin Powder from Jerusalem Artichoke (Helianthus tuberosus L.) Tuber.

    Science.gov (United States)

    Srinameb, Bang-orn; Nuchadomrong, Suporn; Jogloy, Sanun; Patanothai, Aran; Srijaranai, Supalax

    2015-06-01

    The complete procedure for the production of inulin powder from Jerusalem artichoke tubers (JAT) was investigated. The procedure consists of isolation of inulin from JAT, elimination of color from the inulin extract and solidification. Washed tubers were first sliced, dried in a 60 °C oven for 10 h and then milled and sieved into a powder. Inulin was isolated from the JAT powder by hot water extraction using an accelerated solvent extractor (ASE). The effects of temperature and time for the extraction were investigated. The highest extraction efficiency was obtained at the extraction temperature of 80 °C for 20 min. The color of the extract was eliminated using ion exchange process with diethylaminoethyl cellulose as the sorbent. The inulin powder was subsequently obtained by freeze drying. Inulin content and inulin profiles were monitored to evaluate the efficiencies of the complete procedure. The inulin content was indirectly determined by spectrophotometry from free and total fructose measurements using potassium iodide. The inulin profile was monitored using high performance anion exchange chromatography equipped with integrated pulse amperometric detection (HPAEC-PAD). The proposed method provided the inulin production yield of 92.5%. The present procedure is fast, simple and effective for production of inulin powder from JAT. In addition, infrared spectra and some physico-chemical properties of the obtained inulin powder were determined and compared with the standard inulin.

  3. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

    Institute of Scientific and Technical Information of China (English)

    DAI Jianying; ZHANG Yuanli; XIU Zhilong

    2011-01-01

    The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol,an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study,we investigated the use of salting-out extraction(SOE) that employed a K2HPO4/ethanol system consisting of 21% ethanol and 17% K2HPO4(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE,about 98% of solid matters was removed,and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%,respectively,and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.

  4. Effect of temperature on acid hydrolysis of Jerusalem artichoke as raw material for ethanol production

    Directory of Open Access Journals (Sweden)

    Razmovski Radojka N.

    2013-01-01

    Full Text Available Jerusalem artichoke (JA is a low-requirement crop, which does not interfere with food chain, and is a promising carbon source for industrial fermentation. Microbial conversion of such a renewable raw material to useful products, such as ethanol, is an important objective in industrial biotechnology. In this study, ethanol was efficiently produced from the hydrolyzates of JA obtained at different pH values (pH 2.5, pH 3.0 and pH 3.5, temperature (120, 130, 132 and 134°C and hold time (30 and 60 min by Saccharomyces cerevisiae. The efficient degradation of JA by HCl under certain experimental conditions was confirmed by thin-layer chromatography. Ethanol concentration of 7.52% (w/w, which corresponds to 93.89 % of the theoretical yield is achieved by ethanol fermentation of JA hydrolyzate obtained at pH 2.5. [Projekat Ministarstva nauke Republike Srbije, br. TR-31002

  5. Effect of Seawater Stress on Physiological and Biochemical Responses of Five Jerusalem Artichoke Ecotypes

    Institute of Scientific and Technical Information of China (English)

    LONG Xiao-Hua; CHI Jin-He; LIU Ling; LI Qing; LIU Zhao-Pu

    2009-01-01

    Three treatments consisting of 0%,15%,and 30% seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke (Helianthus tuberosus) regarding their responses to seawater stress under a hydroponic culture system.Analyses were done 2,4,and 6 days after treatments.The 15% and 30% seawater treatments reduced the growth rates of roots and shoots of H.tuberosus populations.The activities of superoxide dismutase,peroxidase,and catalase majored in the leaves were stimulated under the seawater stress.The electrolyte leakage and malondialdehyde contents of the leaves were also stimulated owing to seawater stress.The contents of proline and solublesugars in the leaves increased significantly with increasing seawater concentrations.The concentrations of Na+,K+,and Cl-in the aerial parts and roots increased with an increase in the seawater concentration throughout the experimental period.There were ccotypic differences among the five populations of H.tuberosus as evidenced by the analyses of the above items in both aerial parts and roots under seawater treatment.The magnitude of the ecotypic variance components indicated that a substantial proportion of the total variation for these physiological and biochemical responses were owing to ecotype,indicating the possibility of improvement through hybridization and selection.

  6. Artichoke compound cynarin differentially affects the survival, growth and stress response of normal, immortalized and cancerous human cells

    DEFF Research Database (Denmark)

    Gezer, Ceren; Yücecan, Sevinç; Rattan, Suresh Inder Singh

    2015-01-01

    Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. Potential health-beneficial effects of CYN include as being choloretic-cholesterol lowering, hepatoprotective, anti-atherosclerotic, and antioxidative. We have tested the effects of various doses...... of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers haemoxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC) and cervical cancer cells, HeLa. Effects of CYN...

  7. 借助斑马鱼EST数据库从鲤鱼微卫星序列中寻找蛋白编码基因%Searching for Protein-coding Genes Using Microsatellites in Common Carp by Comparing to Zebrafish EST Database

    Institute of Scientific and Technical Information of China (English)

    常玉梅; 匡友谊; 梁利群; 鲁翠云; 何建国; 孙效文

    2008-01-01

    应用in sifico的方法,利用Blastu和Blastx搜索引擎,将鲤鱼微卫星序列与GenBank数据库进行同源序列比对.利用Blastn,将侧翼序列长度>50bp的875个鲤鱼微卫星序列与斑马鱼的EST数据库首先进行比对,结果找到了121个同源序列.随后采用Blastx搜索蛋白质数据库,有94个微卫星位点存在同源蛋白.除了33个假定和3个未知蛋白外,剩余的58个微卫星位点被成功地进行了功能注释,而且其中的7个位点已经定位在了鲤鱼连锁图谱上.另外,通过PCR-SSCP的方法,将两个与鲤鱼微卫星侧翼序列相匹配的斑马鱼EST序列开发成鲤鱼的STS标记,并将其中的一个标记HLJZe33定位到鲤鱼连锁图谱上.以上研究结果表明,通过比较基因组研究,模式生物斑马鱼的很多遗传和基因组资源都可以被利用到鲤鱼的基因组研究中.%In this study,an in silico approach was utilized to identify homologies existing between common carp microsatellite sequences and GenBank database using Biastn and Blastx searches.About 875 microsatellite sites with flanking sequences over 50bp of common carp were first compared to the zebrafish EST database.The results showed that 121 homologies were found using Blastn.Subsequent Blastx searches confirmed 94 sites recorded in the protein database.Except for 33 hypothetical proteins and three unknown proteins,seven out of 58 characterized proteins have been mapped to two linkage maps.In addition,two polymorphic STS markers were developed using matched zebrafish EST sequences by PCR-SSCP method,of which one marker HLJZe33 was mapped successfully.This study Was a pilot for comparative studies between common carp and zebrafish,and the results demonstrated that more genetic and genomic resources of zebrafish can be used for the genome research of common carp.

  8. Database Manager

    Science.gov (United States)

    Martin, Andrew

    2010-01-01

    It is normal practice today for organizations to store large quantities of records of related information as computer-based files or databases. Purposeful information is retrieved by performing queries on the data sets. The purpose of DATABASE MANAGER is to communicate to students the method by which the computer performs these queries. This…

  9. Effect of NO-3-N Enrichment on Seawater Stress Tolerance of Jerusalem Artichoke(Helianthus tuberosus)

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A hydroponic experiment with six treatments,i.e.,0% seawater(control),10% seawater,25% seawater,0% seawater +N(7.5 mmol L-1 NaNO3),10% seawater + N(7.5 mmol L-1 NaNO3),and 25% seawater +N(7.5 mmol L-1 NaNO3),was carried out to study the effect of nitrogen addition on the growth and physiological and biochemical characteristics of Jerusalem artichoke(Helianthus tuberosus)seedlings under seawater stress.The 10% seawater stress treatment had the least effect on plant growth while at 25% seawater growth was significantly inhibited.The malondialdehyde content and electrolyte leakage in leaves under 10% seawater were similar to those of the control,but significantly higher under the 25% seawater stress.The activities of superoxide dismutase,peroxidase and catalase in the leaves increased concomitantly with increasing seawater concentration and time.Proline and soluble-sugars in the leaves and Na+,K+,and Cl- contents in shoots and roots increased significantly with the concentration of seawater increasing.Nitrogen addition resulted in increasing fresh and dry weights of shoots and roots compared with seawater treatment without N.Nitrogen supplemen-tation also significantly enhanced the activities of antioxidant enzymes in leaves.Addition of N to seawater enhanced the contents of proline and soluble-sugars in the leaves and K+ and total-N in the aerial parts and roots of H.tuberosus,but it resulted in declined concentrations of Na+ and Cl- in the aerial parts and roots.Nitrogen addition ameliorated the toxicity of seawater by improving the antioxidative enzymes,accumulating of proline and soluble-sugars,and altering the distribution of inorganic ions in H.tuberosus.

  10. In silco mapping of ESTs from the turkey (Meleagris gallopavo).

    Science.gov (United States)

    Reed, Kent M; Knutson, Todd P; Krueth, Stacy B; Sullivan, Laura R; Chaves, Lee D

    2005-01-01

    Sequence similarity was used to predict the position of expressed sequence tags (ESTs) in the genome of the turkey (Meleagris gallopavo). Turkey EST sequences were compared with the draft assembly of the chicken whole-genome sequence and the chicken EST database by BLASTN. Among the 877 ESTs examined, 788 had significant matches in the chicken genome sequence. Position of orthologous sequences in the chicken genome and the predicted position of the EST loci in the turkey genome are presented Genetic assignments suggest a high level of accuracy for the COMPASS predictions.

  11. In vitro callus-induction in globe artichoke (Cynara cardunculus L. var. scolymus) as a system for the production of caffeoylquinic acids

    NARCIS (Netherlands)

    Menin, B.; Moglia, A.; Comino, C.; Hakkert, J.C.; Lanteri, S.; Beekwilder, M.J.

    2013-01-01

    Globe artichoke (Cynara cardunculus L. var. scolymus) provides a rich dietary source of bio-active compounds derived from phenylpropanoid metabolism, notably caffeoylquinic acids (CQAs) and flavonoids. Micropropagation techniques have been established for this species, but in vitro cultures have not

  12. Variation of inulin content, inulin yield and water use efficiency for inulin yield in Jerusalem artichoke genotypes under different water regimes

    Science.gov (United States)

    The information on genotypic variation for inulin content, inulin yield and water use efficiency of inulin yield (WUEi) in response to drought is limited. This study was to investigate the genetic variability in inulin content, inulin yield and WUEi of Jerusalem artichoke (Helianthus tuberosus L.) ...

  13. First detailed karyo-morphological analysis and molecular cytological study of leafy cardoon and globe artichoke, two multi-use Asteraceae crops

    Directory of Open Access Journals (Sweden)

    Debora Giorgi

    2016-09-01

    Full Text Available Traditionally globe artichoke and leafy cardoon have been cultivated for use as vegetables but these crops are now finding multiple new roles in applications ranging from paper production to cheese preparation and biofuel use, with interest in their functional food potential. So far, their chromosome complements have been poorly investigated and a well-defined karyotype was not available. In this paper, a detailed karyo-morphological analysis and molecular cytogenetic studies were conducted on globe artichoke (Cynara cardunculus Linnaeus, 1753 var. scolymus Fiori, 1904 and leafy cardoon (C. cardunculus Linneaus, 1753 var. altilis De Candolle, 1838. Fluorescent In Situ Hybridization In Suspension (FISHIS was applied to nuclei suspensions as a fast method for screening of labelling probes, before metaphase spread hybridization. Classic Fluorescent In Situ Hybridization (FISH on slide, using repetitive telomeric and ribosomal sequences and Simple Sequence Repeats (SSRs oligonucleotide as probes, identified homologous chromosome relationships and allowed development of molecular karyotypes for both varieties. The close phylogenetic relationship between globe artichoke and cardoon was supported by the very similar karyotypes but clear chromosomal structural variation was detected. In the light of the recent release of the globe artichoke genome sequencing, these results are relevant for future anchoring of the pseudomolecule sequence assemblies to specific chromosomes. In addition, the DNA content of the two crops has been determined by flow cytometry and a fast method for standard FISH on slide and methodological improvements for nuclei isolation are described.

  14. Genetic mapping and characterization of the globe artichoke (+)-germacrene A synthase gene, encoding the first dedicated enzyme for biosynthesis of the bitter sesquiterpene lactone cynaropicrin

    NARCIS (Netherlands)

    Menin, B.; Comino, C.; Portis, E.; Moglia, A.; Cankar, K.; Bouwmeester, H.J.; Lanteri, S.; Beekwilder, M.J.

    2012-01-01

    Globe artichoke (Cynara cardunculus var. scolymus L., Asteraceae) is a perennial crop traditionally consumed as a vegetable in the Mediterranean countries and rich in nutraceutically and pharmaceutically active compounds, including phenolic and terpenoid compounds. Its bitter taste is caused by its

  15. New method for determining sensory shelf life using fuzzy logic: canned marinated artichoke hearts (Cynara scolymus L. case

    Directory of Open Access Journals (Sweden)

    Víctor Vásquez-Villalobos

    2015-06-01

    Full Text Available The sensory preference (sp and shelf life of sensory acceptability (SLSA of canned artichoke hearts were modeled using fuzzy logic (FL and accelerated testing. The artichoke hearts were marinated in oil of sacha inchi (Plukenetia volubilis, soybean (Glycine max and olive (Olea europea; and evaluated using a Ranking test with a semi-trained panel, to identify the best preference both for flavor (f and limpidity (l. We evaluated a global sp through intersection (AND and union (OR fuzzy operations of f and l, using functions of triangular membership with the Mamdani method for defuzzificacion through 25 linguistic rules. The intersection showed the best modeling performance, with the highest sp value at 3.30 for the treatment with sacha inchi (50%, olive (25% and soybean (25% (p << 0.05 oil, which was subjected to accelerated testing at 37 °C, 49 °C, 55 °C and evaluated according to their sensory acceptability (SA through an unstructured scale test in terms of f and l. The SLSA was determined using accelerated testing with FL through intersection fuzzy operation of f and l, triangular membership functions for f and l, and also 25 linguistic rules. A SLSA at 20 ºC was determined for a "high" SA of 296 days, and 569 days for a SA between "high and beginning of medium SA". Both values were lower than the 892 days’ time determined by accelerated testing when evaluating the peroxide index in canned products.

  16. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    International Nuclear Information System (INIS)

    An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). (author)

  17. Influence of cooking conditions on organoleptic and health-related properties of artichokes, green beans, broccoli and carrots.

    Science.gov (United States)

    Guillén, Sofía; Mir-Bel, Jorge; Oria, Rosa; Salvador, María L

    2017-02-15

    Colour, pigments, total phenolic content and antioxidant activity were investigated in artichokes, green beans, broccoli and carrots cooked under different conditions. Domestic induction hobs with temperature control were used to evaluate the effect of boiling, sous-vide cooking and water immersion cooking at temperatures below 100°C on the properties of each vegetable. Sous-vide cooking preserved chlorophyll, carotenoids, phenolic content and antioxidant activity to a greater extent than boiling for all of the vegetables tested and retained colour better, as determined by a(∗). A reduction of only 10-15°C in the cooking temperature was enough to improve the properties of the samples cooked by water immersion, except for green beans. Artichokes and carrots suffered pronounced losses of antioxidant activity during boiling (17.0 and 9.2% retention, respectively), but the stability of this parameter significantly increased with sous-vide cooking (84.9 and 55.3% retention, respectively). PMID:27664628

  18. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Shu, Xikai; Wang, Mei; Liu, Daicheng [College of Life Science, Shandong Normal University, Jinan, Shandong (China); Wang, Daijie; Lin, Xiaojing; Liu, Jianhua; Wang, Xiao; Huang, Luqi, E-mail: wxjn1998@126.com [Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong (China)

    2013-09-01

    An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-{beta}-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-{beta}-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). (author)

  19. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    Directory of Open Access Journals (Sweden)

    Xikai Shu

    2013-01-01

    Full Text Available An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I, 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II, 18.4 mg of luteolin-7-O-β-D-glucoside (compound III, and 33.4 mg of cynarin (compound IV with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS and nuclear magnetic resonance (NMR.

  20. Database Replication

    CERN Document Server

    Kemme, Bettina

    2010-01-01

    Database replication is widely used for fault-tolerance, scalability and performance. The failure of one database replica does not stop the system from working as available replicas can take over the tasks of the failed replica. Scalability can be achieved by distributing the load across all replicas, and adding new replicas should the load increase. Finally, database replication can provide fast local access, even if clients are geographically distributed clients, if data copies are located close to clients. Despite its advantages, replication is not a straightforward technique to apply, and

  1. Probabilistic Databases

    CERN Document Server

    Suciu, Dan; Koch, Christop

    2011-01-01

    Probabilistic databases are databases where the value of some attributes or the presence of some records are uncertain and known only with some probability. Applications in many areas such as information extraction, RFID and scientific data management, data cleaning, data integration, and financial risk assessment produce large volumes of uncertain data, which are best modeled and processed by a probabilistic database. This book presents the state of the art in representation formalisms and query processing techniques for probabilistic data. It starts by discussing the basic principles for rep

  2. Dealer Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The dealer reporting databases contain the primary data reported by federally permitted seafood dealers in the northeast. Electronic reporting was implemented May...

  3. RDD Databases

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database was established to oversee documents issued in support of fishery research activities including experimental fishing permits (EFP), letters of...

  4. National database

    DEFF Research Database (Denmark)

    Kristensen, Helen Grundtvig; Stjernø, Henrik

    1995-01-01

    Artikel om national database for sygeplejeforskning oprettet på Dansk Institut for Sundheds- og Sygeplejeforskning. Det er målet med databasen at samle viden om forsknings- og udviklingsaktiviteter inden for sygeplejen.......Artikel om national database for sygeplejeforskning oprettet på Dansk Institut for Sundheds- og Sygeplejeforskning. Det er målet med databasen at samle viden om forsknings- og udviklingsaktiviteter inden for sygeplejen....

  5. PAVE: Program for assembling and viewing ESTs

    Directory of Open Access Journals (Sweden)

    Bomhoff Matthew

    2009-08-01

    Full Text Available Abstract Background New sequencing technologies are rapidly emerging. Many laboratories are simultaneously working with the traditional Sanger ESTs and experimenting with ESTs generated by the 454 Life Science sequencers. Though Sanger ESTs have been used to generate contigs for many years, no program takes full advantage of the 5' and 3' mate-pair information, hence, many tentative transcripts are assembled into two separate contigs. The new 454 technology has the benefit of high-throughput expression profiling, but introduces time and space problems for assembling large contigs. Results The PAVE (Program for Assembling and Viewing ESTs assembler takes advantage of the 5' and 3' mate-pair information by requiring that the mate-pairs be assembled into the same contig and joined by n's if the two sub-contigs do not overlap. It handles the depth of 454 data sets by "burying" similar ESTs during assembly, which retains the expression level information while circumventing time and space problems. PAVE uses MegaBLAST for the clustering step and CAP3 for assembly, however it assembles incrementally to enforce the mate-pair constraint, bury ESTs, and reduce incorrect joins and splits. The PAVE data management system uses a MySQL database to store multiple libraries of ESTs along with their metadata; the management system allows multiple assemblies with variations on libraries and parameters. Analysis routines provide standard annotation for the contigs including a measure of differentially expressed genes across the libraries. A Java viewer program is provided for display and analysis of the results. Our results clearly show the benefit of using the PAVE assembler to explicitly use mate-pair information and bury ESTs for large contigs. Conclusion The PAVE assembler provides a software package for assembling Sanger and/or 454 ESTs. The assembly software, data management software, Java viewer and user's guide are freely available.

  6. Biological Databases

    Directory of Open Access Journals (Sweden)

    Kaviena Baskaran

    2013-12-01

    Full Text Available Biology has entered a new era in distributing information based on database and this collection of database become primary in publishing information. This data publishing is done through Internet Gopher where information resources easy and affordable offered by powerful research tools. The more important thing now is the development of high quality and professionally operated electronic data publishing sites. To enhance the service and appropriate editorial and policies for electronic data publishing has been established and editors of article shoulder the responsibility.

  7. 菊芋在生物工业中的应用%Applications of the Jerusalem artichoke in the biological industry

    Institute of Scientific and Technical Information of China (English)

    高教琪; 袁文杰; 陈丽杰; 白凤武

    2012-01-01

    Jerusalem artichoke (JA) , as a kind of non-grain crops, has the growth characteristics such as cold-resistance, drought-resistance, high productivity and keeping water and soil. The biorefinery of Jerusalem artichoke is a key technology to solve the problem of exhausted fossil resources, and will keep sustainable development of human beings. In this article, the highlights of biorefinery of the Jerusalem artichoke were reviewed, especially the significant applications in the industry of bioenergy, biomaterial, platform chemical, pharmaceutical and food.%菊芋作为一种非粮作物,具有耐寒、耐旱、繁殖力强、保持水土等生长特性,利用菊芋进行生物炼制是解决目前化石资源日益枯竭,维持人类可持续发展的重要举措.综述了近几年国内外菊芋生物炼制的研究成果,重点介绍了菊芋在生物质能源、平台化合物、医药、食品等工业方面的应用.

  8. Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid.

    Science.gov (United States)

    Wang, Ling-Fei; Wang, Zhi-Peng; Liu, Xiao-Yan; Chi, Zhen-Ming

    2013-11-01

    In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem artichoke tubers without addition of any other components were suitable for citric acid production by the yeast strain. During 10 L fermentation using the extract containing 84.3 g L(-1) total sugars, 68.3 g L(-1) citric acid was produced and the yield of citric acid was 0.91 g g(-1) within 336 h. At the end of the fermentation, 9.2 g L(-1) of residual total sugar and 2.1 g L(-1) of reducing sugar were left in the fermented medium. At the same time, citric acid in the supernatant of the culture was purified. It was found that 67.2 % of the citric acid in the supernatant of the culture was recovered and purity of citric acid in the crystal was 96 %.

  9. Composição química e atividades biológicas das folhas de Cynara scolymus L. (alcachofra) cultivada no Brasil Chemical composition and biological activities of the leaves of Cynara scolymus L. (artichoke) cultivated in Brazil

    OpenAIRE

    Vânia Floriani Noldin; Valdir Cechinel Filho; Franco Delle Monache.; Jean Carlo Benassi; Irma Luiza Christmann; Rozangela Curi Pedrosa; Rosendo Augusto Yunes

    2003-01-01

    The present paper describes the chemical composition and biological activities of artichoke cultivated in Brazil. Our studies demonstrated that glycosyl flavonoids (cynaroside and scolymoside), are the major constituents, along with cynaropicrin, a sesquiterpene lactone, and the triterpene lupeol. Cynarin, which is the main compound described for artichoke, was detected in very low concentration. Hexanic fraction exhibited considerable cytotoxicity and diuretic activities.

  10. Characterization and selection of globe artichoke and cardoon germplasm for biomass, food and biocompound production

    OpenAIRE

    Ciancolini, Anna

    2012-01-01

    L'artichaut et le cardon, appartenant à la famille des Asteraceae (Compositae), sont des plantes pérennes herbacées natives du bassin méditerranéen, et qui sont traditionnellement cultivées comme plantes maraîchères, respectivement pour leurs têtes et leurs cardes. L'Italie est le pays possédant la plus importante collection de germoplasmes autochtones d'artichaut. Dans le centre de l'Italie, le type Romanesco est étendu. Ces dernières années, le développement des techniques in vitro a permis...

  11. AcEST: DK945511 [AcEST

    Lifescience Database Archive (English)

    Full Text Available a new generation of protein database search programs, Nucleic Acids Res. 25:3389... Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  12. AcEST: DK945340 [AcEST

    Lifescience Database Archive (English)

    Full Text Available w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402...g, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  13. AcEST: DK945742 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945742|Adiantum...J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  14. AcEST: DK946712 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic

  15. AcEST: DK958618 [AcEST

    Lifescience Database Archive (English)

    Full Text Available . Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids

  16. AcEST: BP914202 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acid

  17. AcEST: BP911825 [AcEST

    Lifescience Database Archive (English)

    Full Text Available eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Q...iller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  18. AcEST: BP911586 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911586|Adiantum

  19. AcEST: DK946819 [AcEST

    Lifescience Database Archive (English)

    Full Text Available LAST: a new generation of protein database search programs, Nucleic Acids Res. 25...Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search program

  20. AcEST: BP916167 [AcEST

    Lifescience Database Archive (English)

    Full Text Available d David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs... and PSI-BLAST: a new generation of protein database search programs, Nucleic Aci

  1. AcEST: BP917801 [AcEST

    Lifescience Database Archive (English)

    Full Text Available n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917801|Adiantum capillus-ve...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,

  2. AcEST: BP914772 [AcEST

    Lifescience Database Archive (English)

    Full Text Available -BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9147...avid J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  3. AcEST: DK945762 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3...hang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  4. AcEST: DK948732 [AcEST

    Lifescience Database Archive (English)

    Full Text Available f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948..., and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  5. AcEST: DK952487 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3...hang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  6. AcEST: DK946879 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946879|Adiantu... J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  7. Stackfile Database

    Science.gov (United States)

    deVarvalho, Robert; Desai, Shailen D.; Haines, Bruce J.; Kruizinga, Gerhard L.; Gilmer, Christopher

    2013-01-01

    This software provides storage retrieval and analysis functionality for managing satellite altimetry data. It improves the efficiency and analysis capabilities of existing database software with improved flexibility and documentation. It offers flexibility in the type of data that can be stored. There is efficient retrieval either across the spatial domain or the time domain. Built-in analysis tools are provided for frequently performed altimetry tasks. This software package is used for storing and manipulating satellite measurement data. It was developed with a focus on handling the requirements of repeat-track altimetry missions such as Topex and Jason. It was, however, designed to work with a wide variety of satellite measurement data [e.g., Gravity Recovery And Climate Experiment -- GRACE). The software consists of several command-line tools for importing, retrieving, and analyzing satellite measurement data.

  8. EFFECTS OF ARTICHOKE (CYNARA SCOLYMUS L. EXTRACT ADDITION ON MICROBIOLOGICAL AND PHYSICO-CHEMICAL PROPERTIES OF PROBIOTIC YOGURT

    Directory of Open Access Journals (Sweden)

    Jalal Ehsani

    2015-06-01

    Full Text Available In this study, the effects of addition of artichoke (Cynara scolymus L. leaf extract into yogurt (0 or 0.5% on biochemical parameters (pH, titrable acidity and the viability of probiotic bacteria (Lactobacillus acidophilus LA-5, Bifidobacterium lactis BB-12 during fermentation and over 28 days of refrigerated storage (4°C were investigated. Moreover, the amounts of syneresis, total phenolic content, antioxidant activity and sensory attributes of yogurts at the end of fermentation were assessed. Yogurts contained the two yogurt bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus: ABY or only S. thermophilus (ABT as adjunct culture to probiotics. Yogurts containing Cynara scolymus L. (ABT-C and ABY-C had faster acidity increase, shorter incubation time and greater final titrable acidity than control yogurts (ABT and ABY. Also, yogurts containing Cynara scolymus L. had lower syneresis, higher total phenolic content and greater antioxidant activity. ABT-C yogurt had the ever greatest viability of probiotics. In case of samples sensory evaluation, generally, the highest total score was related to ABT yogurt whereas lowest total score belonged to ABT-C yogurt.

  9. Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.

    Science.gov (United States)

    Ruiz-Aceituno, Laura; García-Sarrió, M Jesús; Alonso-Rodriguez, Belén; Ramos, Lourdes; Sanz, M Luz

    2016-04-01

    Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts. MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample). On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques. Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product. To the best of our knowledge this is the first time that these techniques are applied for this purpose.

  10. A Two-Step Nanofiltration Process for the Production of Phenolic-Rich Fractions from Artichoke Aqueous Extracts

    Directory of Open Access Journals (Sweden)

    Alfredo Cassano

    2015-04-01

    Full Text Available Commercial nanofiltration (NF membranes in spiral-wound configuration (NP030 from Microdyn Nadir and Desal DK from GE Water & Process Technologies were used in a sequential design in order to produce a separated fraction of phenolic and sugar compounds from an aqueous artichoke extract. For both membranes, the effect of transmembrane pressure (TMP on the permeation flux was evaluated. In optimized conditions of TMP, the NP030 membrane exhibited high rejections of apigenin, cynarin and chlorogenic acid (higher than 85%; on the other hand, very low rejections of fructose, glucose and sucrose (lower than 4% were measured. Starting from an extract with a total antioxidant activity (TAA of 5.28 mM trolox a retentate fraction with a TAA of 47.75 mM trolox was obtained. The NF permeate from the NP030 membrane was processed with the Desal DK membrane in optimized conditions of TMP producing a permeate stream free of phenolic and sugar compounds. Accordingly, as most part of phenolic compounds was removed in the first NF step, the concentration of sugar compounds in the NF retentate had much higher results than that of phenolic compounds.

  11. Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase.

    Science.gov (United States)

    Kim, D M; Kim, H S

    1992-02-01

    Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chitin-immobilized inulinase in alginate beads. The optimum amounts of both chitin-immobilized inulinase and permeabilized cells for coimmobilization were determined, and operational conditions were optimized. In a continuous stirred tank reactor operation, the maximum productivities for gluconic acid and sorbitol were about 19.2 and 21.3 g/L/h, respectively, at the dilution rate of 0.23 h(-1) and the substrate concentration of 20%, but operational stability was low because of the abrasion of the beads. As an approach to increase the operational stability, a recycle packed-bed reactor (RPBR) was employed. In RPBR operation, the maximum productivities for gluconic acid and sorbitol were found to be 23.4 and 26.0 g/L/h, respectively, at the dilution rate of 0.35 h(-1) and the substrate concentration of 20% when the recirculation rate was fixed at 900 mL/h. Coimmobilized enzymes were stable for 250 h in a recycle packed-bed reactor without any loss of activity, while half-life in a continuous stirred tank reactor (CSTR) was observed to be about 150 h.

  12. Optimization of microwave-assisted drying of Jerusalem artichokes (Helianthus tuberosus L. by response surface methodology and genetic algorithm

    Directory of Open Access Journals (Sweden)

    E. KARACABEY

    2016-03-01

    Full Text Available The objective of the present study was to investigate microwave-assisted drying of Jerusalem artichoke tubers to determine the effects of the processing conditions. Drying time (DT and effectivemoisture diffusivity (EMD were determined to evaluate the drying process in terms of dehydration performance, whereas the rehydration ratio (RhR was considered as a significant quality index. A pretreatment of soaking in a NaCl solution was applied before all trials. The output power of the microwave oven, slice thickness and NaCl concentration of the pretreatment solution werethe three investigated parameters. The drying process was accelerated by altering the conditions while obtaining a higher quality product. For optimization of the drying process, response surface methodology (RSM and genetic algorithms (GA were used. Model adequacy was evaluated for each corresponding mathematical expression developed for interested responses by RSM. The residual of the model obtained by GA was compared to that of the RSM model. The GA was successful in high-performance prediction and produced results similar to those of RSM. The analysis and results of the present study show that both RSM and GA models can be used in cohesion to gain insight into the bioprocessing system.

  13. Salinity altered root distribution and increased diversity of bacterial communities in the rhizosphere soil of Jerusalem artichoke.

    Science.gov (United States)

    Yang, Hui; Hu, Jinxiang; Long, Xiaohua; Liu, Zhaopu; Rengel, Zed

    2016-01-01

    The interaction between roots and bacterial communities in halophytic species is poorly understood. Here, we used Jerusalem artichoke cultivar Nanyu 1 (NY-1) to characterise root distribution patterns and determine diversity and abundance of bacteria in the rhizosphere soil under variable salinity. Root growth was not inhibited within the salinity range 1.2 to 1.9 g salt/kg, but roots were mainly confined to 0-20 cm soil layer vertically and 0-30 cm horizontally from the plant centre. Root concentrations of K(+), Na(+), Mg(2+) and particularly Ca(2+) were relatively high under salinity stress. High salinity stress decreased soil invertase and catalase activity. Using a next-generation, Illumina-based sequencing approach, we determined higher diversity of bacteria in the rhizosphere soil at high than low salinity. More than 15,500 valid reads were obtained, and Proteobacteria, Acidobacteria, Bacteroidetes and Actinobacteria predominated in all samples, accounting for >80% of the reads. On a genus level, 636 genera were common to the low and high salinity treatments at 0-5 cm and 5-10 cm depth. The abundance of Steroidobacter and Sphingomonas was significantly decreased by increasing salinity. Higher Shannon and Chao 1 indices with increasing severity of salt stress indicated that high salt stress increased diversity in the bacterial communities. PMID:26852800

  14. Database development and management

    CERN Document Server

    Chao, Lee

    2006-01-01

    Introduction to Database Systems Functions of a DatabaseDatabase Management SystemDatabase ComponentsDatabase Development ProcessConceptual Design and Data Modeling Introduction to Database Design Process Understanding Business ProcessEntity-Relationship Data Model Representing Business Process with Entity-RelationshipModelTable Structure and NormalizationIntroduction to TablesTable NormalizationTransforming Data Models to Relational Databases .DBMS Selection Transforming Data Models to Relational DatabasesEnforcing ConstraintsCreating Database for Business ProcessPhysical Design and Database

  15. Nursery inoculation with the arbuscular mycorrhizal fungus Glomus viscosum and its effect on the growth and physiology of hybrid artichoke seedlings

    Directory of Open Access Journals (Sweden)

    Angela Campanelli

    2011-07-01

    Full Text Available Most nurseries operating in Italy adopt high technologies and produce transplants that well suit and satisfy the grower’s need to produce high value crops. Mycorrhizas are discussed as a tool for improving and developing plant production in the nursery. Much research has been carried out on mycorrhizal symbiosis and we now know more about the symbiontic relationship between fungi and host plants. Plants receive numerous benefits from this symbiosis which are more macroscopic the earlier in the ontogenetic cycle this symbiosis is established. Therefore, it appears that the most effective period in which the inoculum should be made corresponds to the in-nursery growing stage. The earlier the plant is inoculated, the more evident the effect will be. In this study, several aspects related to the physiological foundations of arbuscular mycorrhiza in artichoke plants are presented. The main goal was to study the effects of mycorrhiza on the growth and physiological parameters of three hybrids of artichokes growing in the nursery. The experimental 3¥2 design included two treatments (with or without arbuscular mycorrhizal fungi and three hybrids of artichokes marketed by Nunhems (Opal F1, Madrigal F1, Concerto F1. Mycorrhizal plants have greater shoot length, leaf area, shoot and root fresh and dry mass, and root density. This also corresponded with increased photosynthetic rates and stomatal conductance of mycorrhizal plants. Mycorrhizal colonization improves relative water content and increases proline concentration in vegetal tissue. Inoculation produced the most beneficial effect on hybrid Madrigal F1 and on hybrid Opal F1; the best mycorrhizal affinity was enhanced when compared to hybrid Concerto F1. The results showed that mycorrhizal symbiosis stimulated the growth of inoculated seedlings providing a qualitatively good propagation material.

  16. AcEST: BP920571 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tein homolog OS=... 34 0.62 sp|Q9Y239|NOD1_HUMAN Nucleotide-binding oligomerization domain-c... 33 1.1 sp|Q5FWL4|EST2A_XENLA Extended...ITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRII 951 >sp|Q5FWL4|EST2A_XENLA Extended synaptotagmin-2-A OS=Xenopu

  17. Free-range pigs foraging on Jerusalem artichokes (Helianthus tuberosus L.) – Effect of feeding strategy on growth, feed conversion and animal behaviour

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Horsted, Klaus; Hermansen, John Erik

    2013-01-01

    The nutritional contributions from free-range foraging, growth, feed conversion and behaviour were investigated in 36 growing pigs foraging on Jerusalem artichokes (JA) and fed concentrates restrictedly (30% of energy recommendations) or ad libitum. Compared to the ad libitum fed pigs, the pigs fed...... restrictedly had a significant lower daily gain (560 vs. 1224 g pig−1), improved feed conversion ratio (17.6 vs. 42.8 MJ ME concentrate kg−1 live weight gain) and spent more time foraging JA tubers (7.9 vs. 1.1%). It is estimated that pigs fed restrictedly found approximately 60% of their energy requirement...... from foraging in the range....

  18. Databases and their application

    NARCIS (Netherlands)

    E.C. Grimm; R.H.W Bradshaw; S. Brewer; S. Flantua; T. Giesecke; A.M. Lézine; H. Takahara; J.W.,Jr Williams

    2013-01-01

    During the past 20 years, several pollen database cooperatives have been established. These databases are now constituent databases of the Neotoma Paleoecology Database, a public domain, multiproxy, relational database designed for Quaternary-Pliocene fossil data and modern surface samples. The poll

  19. Unit 66 - Database Creation

    OpenAIRE

    Unit 61, CC in GIS; National Center for Geographic Information and Analysis (UC Santa Barbara, SUNY at Buffalo, University of Maine)

    1990-01-01

    This unit examines the planning and management issues involved in the physical creation of the database. It describes some issues in database creation, key hardware parameters of the system, partitioning the database for tiles and layers and converting data for the database. It illustrates these through an example from the Flathead National Forest in northwestern Montana, where a resource management database was required.

  20. AcEST: DK944792 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids R...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94479...hang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  1. AcEST: DK946525 [AcEST

    Lifescience Database Archive (English)

    Full Text Available I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946..., and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= DK946525|Adiantum capillus-veneris mR

  2. AcEST: BP917904 [AcEST

    Lifescience Database Archive (English)

    Full Text Available eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917904|Adiantum capil...ler, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  3. AcEST: DK946127 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids R...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94612...hang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  4. AcEST: DK956634 [AcEST

    Lifescience Database Archive (English)

    Full Text Available d David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, ...grams, Nucleic Acids Res. 25:3389-3402. Query= DK956634|...ng, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search pro

  5. AcEST: BP914015 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914015|Adiantum cap

  6. AcEST: BP917157 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91715... Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  7. AcEST: DK943809 [AcEST

    Lifescience Database Archive (English)

    Full Text Available avid J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nuc...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Qu

  8. AcEST: BP913388 [AcEST

    Lifescience Database Archive (English)

    Full Text Available iller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-34

  9. AcEST: DK945539 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2... Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progra...ms, Nucleic Acids Res. 25:3389-3402. Query= DK945539|Adi

  10. AcEST: DK947829 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, N...g, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= DK947829|A

  11. AcEST: DK946060 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Re...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946060...ang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of p

  12. AcEST: DK944295 [AcEST

    Lifescience Database Archive (English)

    Full Text Available SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res...grams, Nucleic Acids Res. 25:3389-3402. Query= DK944295|...ng, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search pro

  13. AcEST: DK944084 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3...eng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  14. AcEST: BP920861 [AcEST

    Lifescience Database Archive (English)

    Full Text Available er, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402.

  15. AcEST: DK945348 [AcEST

    Lifescience Database Archive (English)

    Full Text Available T: a new generation of protein database search programs, Nucleic Acids Res. 25:33...ng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  16. AcEST: DK943845 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943845|Adiantum capillus-veneris...ler, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  17. AcEST: BP920933 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402.... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920933|Adiantum capillus-ven

  18. AcEST: BP911599 [AcEST

    Lifescience Database Archive (English)

    Full Text Available SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91...eng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  19. AcEST: DK946772 [AcEST

    Lifescience Database Archive (English)

    Full Text Available -BLAST: a new generation of protein database search programs, Nucleic Acids Res. ..., Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progr...ams, Nucleic Acids Res. 25:3389-3402. Query= DK946772|Ad

  20. Acerca de este sitio web

    Science.gov (United States)

    Página de guía que permite al lector entender la forma en que está organizado el sitio web del Instituto Nacional del Cáncer (NCI), las categorías de información disponibles y las políticas que rigen este sitio web.

  1. World Religion Database

    OpenAIRE

    Dekker, Jennifer

    2009-01-01

    This article reviews the new database released by Brill entitled World Religion Database (WRD). It compares WRD to other religious demography tools available and rates the database on a 5 point scale.

  2. Collecting Taxes Database

    Data.gov (United States)

    US Agency for International Development — The Collecting Taxes Database contains performance and structural indicators about national tax systems. The database contains quantitative revenue performance...

  3. USAID Anticorruption Projects Database

    Data.gov (United States)

    US Agency for International Development — The Anticorruption Projects Database (Database) includes information about USAID projects with anticorruption interventions implemented worldwide between 2007 and...

  4. Effects of glucose irradiated by high doses of 60cobalt gamma rays, and of some products of glucose radiolysis on the growth of Jerusalem Artichoke tissue and potato shoots culture in vitro

    International Nuclear Information System (INIS)

    Glucose, irradiated in dry conditions by gamma rays from 5.105 to 107 rad, and incorporated into culture medium, inhibits growth and, simultaneously, increases rhizogenesis of Jerusalem Artichoke tissue in culture. Tuberisation of potato shoots grown in vitro is delayed and partially inhibited. Some substances which result from radiolysis of sugars give the same results, but only at higher concentrations

  5. 菊芋对四川泡菜储藏期间品质变化影响的研究%Study on the Effects of Jerusalem Artichoke on the Quality Changes in the Natural Fermentation of Pickle during Storage

    Institute of Scientific and Technical Information of China (English)

    刘晓莉

    2012-01-01

    Jerusalem artichoke was rich in amino acid, sugar and vitamin, and used for curing pickles or pickled made. Merchant artichoke, Chinese cabbage, radish as raw materials in the salt concentration was 6% , under natural fer-mentation conditions, the comparison experiment of pickle contend Jerusalem artichoke and without was carried out. The results showed that adding the Jerusalem artichoke had little effects on the pH value and total acidity, but increased the ni-trite and ammonia nitrogen content significantly.%菊芋富含氨基酸、糖分和维生素,多用来腌制咸菜或制成泡菜。以市售菊芋、白菜、萝b为原料在食盐浓度为6%、自然发酵条件下的含菊芋的泡菜和不含菊芋的泡菜进行比较实验,实验结果表明:添加菊芋对泡菜pH值、总酸度影响不大,明显增加了亚硝酸盐和氨态氮含量。

  6. Study of self-pollination and capitula characteristics in globe artichoke (Cynara cardunculus var. scolymus Hayek L. under different irrigation regimes

    Directory of Open Access Journals (Sweden)

    Nouraei, Sina

    2016-07-01

    Full Text Available In order to estimate the drought effects on capitula characteristics and self-pollination of globe artichoke (Cynara cardunculus var. scolymus Hayek L., the randomized complete block design was carried out with three irrigation regimes (20 %, 50 % and 80 % depletion of soil available water and six replicates. The artichoke is mostly open-pollinated, however, after covering the buds and isolation of flowers to prevent cross pollination, 1.79 % self-pollination was observed and this amount was not affected by different irrigation regimes. In stress conditions (50 % and 80 % water depletion as well as non-stress condition (20 % water depletion, plants with respectively one and two medium capitula and without small capitula had most relative frequencies in the population and drought stress increased these relative frequencies by reducing the number of medium and small capitula in plants. In addition, Capitula size and dry weight were significantly affected by water stress. Water shortage induced severe decrease in length and dry weight of all capitula including large, medium and small, although capitula width was less affected by water deficit and only slight decline in medium (12.5 % and small capitula (23.7 % was observed under severe stress condition.

  7. Judy Estes Hall (1940-2015).

    Science.gov (United States)

    Sammons, Morgan T; Boucher, Andrew

    2016-01-01

    Presents an obituary for Judy Estes Hall, who passed away on November 24, 2015. Hall served as the Executive Officer of the National Register of Health Service Psychologists until her retirement in 2013. She is a recognized expert in the development of education and training standards for the profession of psychology, she also made significant contributions in the field of international psychology, where she was a renowned expert in cross-national credentialing and an advocate for commonality in licensing standards. She was the coauthor of one edited volume and author of more than 60 journal articles, book chapters, and professional publications. A passionate advocate for the advancement of women in psychology, a devoted mother and grandmother, a connoisseur of wine and international traveler extraordinaire, she touched the personal and professional lives of many. (PsycINFO Database Record PMID:27504582

  8. Judy Estes Hall (1940-2015).

    Science.gov (United States)

    Sammons, Morgan T; Boucher, Andrew

    2016-01-01

    Presents an obituary for Judy Estes Hall, who passed away on November 24, 2015. Hall served as the Executive Officer of the National Register of Health Service Psychologists until her retirement in 2013. She is a recognized expert in the development of education and training standards for the profession of psychology, she also made significant contributions in the field of international psychology, where she was a renowned expert in cross-national credentialing and an advocate for commonality in licensing standards. She was the coauthor of one edited volume and author of more than 60 journal articles, book chapters, and professional publications. A passionate advocate for the advancement of women in psychology, a devoted mother and grandmother, a connoisseur of wine and international traveler extraordinaire, she touched the personal and professional lives of many. (PsycINFO Database Record

  9. AcEST: BP914187 [AcEST

    Lifescience Database Archive (English)

    Full Text Available d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= B... Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progra...ms, Nucleic Acids Res. 25:3389-3402. Query= BP914187|Adiantum capillus-veneris mRNA

  10. AcEST: BP911980 [AcEST

    Lifescience Database Archive (English)

    Full Text Available and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query=...g, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= BP911980|Adiantum capillus-veneris mR

  11. AcEST: DK950385 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, N...BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. ...APREERQ--- 734 Query: 331 SPRGARAERSPRLCAEFCPLTEEEEVLVFADNDTKTNDALQD----SDLISPRAMSLRSV 498 PR R ER PR ++EEEE...TEEEEVLVFADNDTKTNDALQD----SDLISPRAMSLRS 495 + PR R ER PR ++EEEEVL+ ND + ND QD S+ ...738 Query: 331 SPRGARAERSPRLCAEFCPLTEEEEVLVFADNDTKTNDALQD----SDLISPRAMSLRSV 498 P

  12. 盐碱土壤对菊芋菊糖含量的影响%Effects of Saline-alkali Soils on Inulin Content in Jerusalem artichokes

    Institute of Scientific and Technical Information of China (English)

    周东; 隋丹; 于涛; 蔺吉祥

    2014-01-01

    为明确盐碱土壤对菊芋(Helianthus tuberosus )中菊糖含量的影响,利用高效液相色谱法对农田土壤与盐碱土壤上生长的7个品系菊芋的菊糖含量进行分析,盐碱土壤包括轻度盐碱土壤与重度盐碱土壤。结果表明:生长于盐碱土壤的菊芋的菊糖含量均低于农田土壤,其中菊芋品系2、品系5和品系7在轻度盐碱土壤中生长时菊糖含量高于重度盐碱土壤;菊芋品系1、品系4和品系6在重度盐碱土壤中生长时菊糖含量高于轻度盐碱土壤。7个品系中,品系4的菊糖含量在盐碱土壤下降低最多,品系6的菊糖含量在盐碱土壤下降低最少,但其含量在三种生长条件下是所有品系中最低的;品系5的菊糖含量虽然在盐碱土壤下也有所降低,但其含量在三种生长条件下都是所有品系中最高的,推测品系5比较适合在盐碱土壤上生长,在改良盐碱地时可优先选用。%To clarify the effects of saline soils on inulin content in Jerusalem artichokes,we analyze the inulin content of seven strains of Jerusalem artichokes grown on farmland and saline soils,which in-clude mild saline soils and severe saline soils by HPLC.The results show that the inulin content in Je-rusalem artichokes grown on the saline soils is lower than that on the farmland.It is also found that the inulin content in Jerusalem artichokes strain 2,strain 5,and strain 7 grown on the mild saline soils is higher than that on the severe saline soils and the inulin content in Jerusalem artichokes strain 1,strain 4,strain 6 grown on the severe saline soils is higher than that on the mild saline soil.The in-ulin content of strain 4 declines the most on the saline soils in the seven strains.The inulin content of strain 6 declines the least on the saline soils,but its inulin content is the lowest in the seven strains under the three growing conditions.Although the inulin content of strain 5 also declines on the saline

  13. Development and annotation of perennial Triticeae ESTs and SSR markers.

    Science.gov (United States)

    Bushman, B Shaun; Larson, Steve R; Mott, Ivan W; Cliften, Paul F; Wang, Richard R-C; Chatterton, N Jerry; Hernandez, Alvaro G; Ali, Shahjahan; Kim, Ryan W; Thimmapuram, Jyothi; Gong, George; Liu, Lei; Mikel, Mark A

    2008-10-01

    Triticeae contains hundreds of species of both annual and perennial types. Although substantial genomic tools are available for annual Triticeae cereals such as wheat and barley, the perennial Triticeae lack sufficient genomic resources for genetic mapping or diversity research. To increase the amount of sequence information available in the perennial Triticeae, three expressed sequence tag (EST) libraries were developed and annotated for Pseudoroegneria spicata, a mixture of both Elymus wawawaiensis and E. lanceolatus, and a Leymus cinereus x L. triticoides interspecific hybrid. The ESTs were combined into unigene sets of 8 780 unigenes for P. spicata, 11 281 unigenes for Leymus, and 7 212 unigenes for Elymus. Unigenes were annotated based on putative orthology to genes from rice, wheat, barley, other Poaceae, Arabidopsis, and the non-redundant database of the NCBI. Simple sequence repeat (SSR) markers were developed, tested for amplification and polymorphism, and aligned to the rice genome. Leymus EST markers homologous to rice chromosome 2 genes were syntenous on Leymus homeologous groups 6a and 6b (previously 1b), demonstrating promise for in silico comparative mapping. All ESTs and SSR markers are available on an EST information management and annotation database (http://titan.biotec.uiuc.edu/triticeae/). PMID:18923529

  14. The ground-based solar observations database BASS 2000

    OpenAIRE

    Paletou, F; Lafon, M.; Maeght, P.; Grimaud, F.; Louge, T.; Aboudarham, J.

    2007-01-01

    BASS 2000 is the French solar database for ground-based instruments. We describe hereafter our organization, our tasks and the products we can deliver to the international community. Our prospects cover data mining into the THeMIS archive, a participation to the EST endeavour and the creation and curation of the ESPaDOnS/NARVAL stellar spectra database.

  15. Development, chromosome location and genetic mapping of EST-SSR markers in wheat

    Institute of Scientific and Technical Information of China (English)

    CHEN Haimei; LI Linzhi; WEI Xianyun; LI Sishen; LEI Tiandong; HU Haizhou; WANG Honggang; ZHANG Xiansheng

    2005-01-01

    A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2―5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework map previously constructed using recombinant inbred lines.

  16. KALIMER database development

    International Nuclear Information System (INIS)

    KALIMER database is an advanced database to utilize the integration management for liquid metal reactor design technology development using Web applications. KALIMER design database is composed of results database, Inter-Office Communication (IOC), 3D CAD database, and reserved documents database. Results database is a research results database during all phase for liquid metal reactor design technology development of mid-term and long-term nuclear R and D. IOC is a linkage control system inter sub project to share and integrate the research results for KALIMER. 3D CAD database is a schematic overview for KALIMER design structure. And reserved documents database is developed to manage several documents and reports since project accomplishment

  17. KALIMER database development

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Kwan Seong; Lee, Yong Bum; Jeong, Hae Yong; Ha, Kwi Seok

    2003-03-01

    KALIMER database is an advanced database to utilize the integration management for liquid metal reactor design technology development using Web applications. KALIMER design database is composed of results database, Inter-Office Communication (IOC), 3D CAD database, and reserved documents database. Results database is a research results database during all phase for liquid metal reactor design technology development of mid-term and long-term nuclear R and D. IOC is a linkage control system inter sub project to share and integrate the research results for KALIMER. 3D CAD database is a schematic overview for KALIMER design structure. And reserved documents database is developed to manage several documents and reports since project accomplishment.

  18. ESTs and EST-linked polymorphisms for genetic mapping and phylogenetic reconstruction in the guppy, Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    Tripathi Namita

    2007-08-01

    Full Text Available Abstract Background The guppy, Poecilia reticulata, is a well-known model organism for studying inheritance and variation of male ornamental traits as well as adaptation to different river habitats. However, genomic resources for studying this important model were not previously widely available. Results With the aim of generating molecular markers for genetic mapping of the guppy, cDNA libraries were constructed from embryos and different adult organs to generate expressed sequence tags (ESTs. About 18,000 ESTs were annotated according to BLASTN and BLASTX results and the sequence information from the 3' UTRs was exploited to generate PCR primers for re-sequencing of genomic DNA from different wild type strains. By comparison of EST-linked genomic sequences from at least four different ecotypes, about 1,700 polymorphisms were identified, representing about 400 distinct genes. Two interconnected MySQL databases were built to organize the ESTs and markers, respectively. A robust phylogeny of the guppy was reconstructed, based on 10 different nuclear genes. Conclusion Our EST and marker databases provide useful tools for genetic mapping and phylogenetic studies of the guppy.

  19. Cloud Databases: A Paradigm Shift in Databases

    Directory of Open Access Journals (Sweden)

    Indu Arora

    2012-07-01

    Full Text Available Relational databases ruled the Information Technology (IT industry for almost 40 years. But last few years have seen sea changes in the way IT is being used and viewed. Stand alone applications have been replaced with web-based applications, dedicated servers with multiple distributed servers and dedicated storage with network storage. Cloud computing has become a reality due to its lesser cost, scalability and pay-as-you-go model. It is one of the biggest changes in IT after the rise of World Wide Web. Cloud databases such as Big Table, Sherpa and SimpleDB are becoming popular. They address the limitations of existing relational databases related to scalability, ease of use and dynamic provisioning. Cloud databases are mainly used for data-intensive applications such as data warehousing, data mining and business intelligence. These applications are read-intensive, scalable and elastic in nature. Transactional data management applications such as banking, airline reservation, online e-commerce and supply chain management applications are write-intensive. Databases supporting such applications require ACID (Atomicity, Consistency, Isolation and Durability properties, but these databases are difficult to deploy in the cloud. The goal of this paper is to review the state of the art in the cloud databases and various architectures. It further assesses the challenges to develop cloud databases that meet the user requirements and discusses popularly used Cloud databases.

  20. Logical database design principles

    CERN Document Server

    Garmany, John; Clark, Terry

    2005-01-01

    INTRODUCTION TO LOGICAL DATABASE DESIGNUnderstanding a Database Database Architectures Relational Databases Creating the Database System Development Life Cycle (SDLC)Systems Planning: Assessment and Feasibility System Analysis: RequirementsSystem Analysis: Requirements Checklist Models Tracking and Schedules Design Modeling Functional Decomposition DiagramData Flow Diagrams Data Dictionary Logical Structures and Decision Trees System Design: LogicalSYSTEM DESIGN AND IMPLEMENTATION The ER ApproachEntities and Entity Types Attribute Domains AttributesSet-Valued AttributesWeak Entities Constraint

  1. AcEST: BP915586 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 10 OS=Homo s... 29 7.3 sp|Q5M7N9|ESYT3_XENTR Extended synaptotagmin-3 OS=Xenopus tropic... 29 7.3 sp|O43610|...SPY3_HUMAN Protein sprouty homolog 3 OS=Homo sapiens G... 29 9.6 sp|Q7ZWU7|EST2B_XENLA Extended... synaptotagmin-2-B OS=Xenopus laev... 29 9.6 sp|Q5FWL4|EST2A_XENLA Extended synaptotagmin-2-A ...EDISKEQ 501 Query: 319 FYPFHLAKL 345 P HL++L Sbjct: 502 LLPRHLSQL 510 >sp|Q5M7N9|ESYT3_XENTR Extended synapt... S Sbjct: 72 LQPLPQHLSQ--SSIASSMSHSTTAS 95 >sp|Q7ZWU7|EST2B_XENLA Extended synapt

  2. AcEST: DK952058 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 09 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2-B OS=Xenopus laev... 63 2e-09 sp|Q5FWL4|EST2A_XENLA Extended...=... 62 2e-09 sp|Q3TZZ7|ESYT2_MOUSE Extended synaptotagmin-2 OS=Mus musculus G... 62 3e-09 sp|Q7TNF0|DOC2A_M... OS=Mus musculus... 61 5e-09 sp|A0FGR8|ESYT2_HUMAN Extended synaptotagmin-2 OS=Ho

  3. EST Express: PHP/MySQL based automated annotation of ESTs from expression libraries

    Directory of Open Access Journals (Sweden)

    Pardinas Jose R

    2008-04-01

    Full Text Available Abstract Background Several biological techniques result in the acquisition of functional sets of cDNAs that must be sequenced and analyzed. The emergence of redundant databases such as UniGene and centralized annotation engines such as Entrez Gene has allowed the development of software that can analyze a great number of sequences in a matter of seconds. Results We have developed "EST Express", a suite of analytical tools that identify and annotate ESTs originating from specific mRNA populations. The software consists of a user-friendly GUI powered by PHP and MySQL that allows for online collaboration between researchers and continuity with UniGene, Entrez Gene and RefSeq. Two key features of the software include a novel, simplified Entrez Gene parser and tools to manage cDNA library sequencing projects. We have tested the software on a large data set (2,016 samples produced by subtractive hybridization. Conclusion EST Express is an open-source, cross-platform web server application that imports sequences from cDNA libraries, such as those generated through subtractive hybridization or yeast two-hybrid screens. It then provides several layers of annotation based on Entrez Gene and RefSeq to allow the user to highlight useful genes and manage cDNA library projects.

  4. AcEST: BP912094 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_A09 564 Adiantum capillus-veneris mRNA. clone: YMU001_000015_A09. BP9120...94 CL2967Contig1 Show BP912094 Clone id YMU001_000015_A09 Library YMU01 Length 564 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000015_A09. Accession BP912094 Tissue type prothallium Developmental stag... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912094|Adiantum c...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  5. AcEST: BP912061 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_F01 532 Adiantum capillus-veneris mRNA. clone: YMU001_000012_F01. BP912061 - Show BP9120...is mRNA. clone: YMU001_000012_F01. Accession BP912061 Tissue type prothallium Developmental stage - Contig I...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  6. AcEST: BP912025 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_B09 484 Adiantum capillus-veneris mRNA. clone: YMU001_000012_B09. BP9120...25 CL1441Contig1 Show BP912025 Clone id YMU001_000012_B09 Library YMU01 Length 484 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_B09. Accession BP912025 Tissue type prothallium Developmental stag...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  7. AcEST: BP921208 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000147_A03 436 Adiantum capillus-veneris mRNA. clone: YMU001_000147_A03. BP921208 - Show BP92120...is mRNA. clone: YMU001_000147_A03. Accession BP921208 Tissue type prothallium Developmental stage - Contig I..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92120... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921208|Adiantum c

  8. AcEST: DK962120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK962120 CL775Contig1 Show DK962120 Clone id TST39A01NGRL0013_A06 Library TST39 Length 3...25 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_A06. 5' end sequence. Accession DK962120... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962120...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962120|Adiantum ca

  9. AcEST: BP912073 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_G04 498 Adiantum capillus-veneris mRNA. clone: YMU001_000012_G04. BP9120...73 CL1947Contig1 Show BP912073 Clone id YMU001_000012_G04 Library YMU01 Length 498 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_G04. Accession BP912073 Tissue type prothallium Developmental stag... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2

  10. AcEST: BP912074 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_G05 524 Adiantum capillus-veneris mRNA. clone: YMU001_000012_G05. BP912074 - Show BP9120...is mRNA. clone: YMU001_000012_G05. Accession BP912074 Tissue type prothallium Developmental stage - Contig I...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...ength = 1129 Score = 48.9 bits (115), Expect = 2e-04 Identities = 28/120 (23%), Positives = 64/120 (53%) Fra

  11. AcEST: BP912123 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D05 496 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D05. BP91212...3 CL498Contig1 Show BP912123 Clone id YMU001_000015_D05 Library YMU01 Length 496 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000015_D05. Accession BP912123 Tissue type prothallium Developmental stage...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91212...3|Adiantum capillus-veneris mRNA, clone: YMU001_000015_D05. (478 letters) Database: uniprot_sprot.fasta 412

  12. AcEST: BP912912 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000024_C05 413 Adiantum capillus-veneris mRNA. clone: YMU001_000024_C05. BP912912... CL1433Contig1 Show BP912912 Clone id YMU001_000024_C05 Library YMU01 Length 413 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000024_C05. Accession BP912912 Tissue type prothallium Developmental stag... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912912|Adiantum capillus-ven...eris mRNA, clone: YMU001_000024_C05. (413 letters) Database: uniprot_sprot.fasta 412

  13. AcEST: BP912212 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000016_D11 457 Adiantum capillus-veneris mRNA. clone: YMU001_000016_D11. BP912212... CL1085Contig1 Show BP912212 Clone id YMU001_000016_D11 Library YMU01 Length 457 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000016_D11. Accession BP912212 Tissue type prothallium Developmental stag...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912212...|Adiantum capillus-veneris mRNA, clone: YMU001_000016_D11. (457 letters) Database: uniprot_sprot.fasta 412

  14. AcEST: BP912312 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000017_F01 489 Adiantum capillus-veneris mRNA. clone: YMU001_000017_F01. BP912312... CL1779Contig1 Show BP912312 Clone id YMU001_000017_F01 Library YMU01 Length 489 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000017_F01. Accession BP912312 Tissue type prothallium Developmental stag...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912312...|Adiantum capillus-veneris mRNA, clone: YMU001_000017_F01. (489 letters) Database: uniprot_sprot.fasta 412

  15. AcEST: BP912128 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D10 477 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D10. BP91212...8 CL2328Contig1 Show BP912128 Clone id YMU001_000015_D10 Library YMU01 Length 477 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000015_D10. Accession BP912128 Tissue type prothallium Developmental stag... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91212...8|Adiantum capillus-veneris mRNA, clone: YMU001_000015_D10. (461 letters) Database: uniprot_sprot.fasta 412

  16. AcEST: BP917117 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000096_B11 125 Adiantum capillus-veneris mRNA. clone: YMU001_000096_B11. BP917117... CL2704Contig1 Show BP917117 Clone id YMU001_000096_B11 Library YMU01 Length 125 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000096_B11. Accession BP917117 Tissue type prothallium Developmental stag...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917117...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917117

  17. AcEST: BP920166 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G12 149 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G12. BP920166 - Show BP92016...is mRNA. clone: YMU001_000133_G12. Accession BP920166 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920166|Adiantum capillus-veneris

  18. AcEST: BP920169 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_H03 505 Adiantum capillus-veneris mRNA. clone: YMU001_000133_H03. BP920169 - Show BP92016...is mRNA. clone: YMU001_000133_H03. Accession BP920169 Tissue type prothallium Developmental stage - Contig I...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920169|Adiantum cap

  19. AcEST: BP920161 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G06 542 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G06. BP920161 - Show BP92016...is mRNA. clone: YMU001_000133_G06. Accession BP920161 Tissue type prothallium Developmental stage - Contig I...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9201...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016

  20. AcEST: BP920165 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G11 301 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G11. BP920165 - Show BP92016...is mRNA. clone: YMU001_000133_G11. Accession BP920165 Tissue type prothallium Developmental stage - Contig I...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920165...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016

  1. AcEST: BP919949 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000131_B01 318 Adiantum capillus-veneris mRNA. clone: YMU001_000131_B01. BP91994...9 CL2311Contig1 Show BP919949 Clone id YMU001_000131_B01 Library YMU01 Length 318 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000131_B01. Accession BP919949 Tissue type prothallium Developmental stag...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91994...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91994

  2. AcEST: BP914814 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000063_B08 487 Adiantum capillus-veneris mRNA. clone: YMU001_000063_B08. BP914814 - Show BP914814...is mRNA. clone: YMU001_000063_B08. Accession BP914814 Tissue type prothallium Developmental stage - Contig I...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914814...|Adiantum capillus-veneris mRNA, clone: YMU001_000063_B08. (487 letters) Database: uniprot_sprot.f...-trehalose-phosphate sy... 60 7e-15 sp|O14081|TPSX_SCHPO Putative alpha,alpha-trehalose-phosphate sy... 79 1e-14

  3. AcEST: BP921414 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000149_E11 396 Adiantum capillus-veneris mRNA. clone: YMU001_000149_E11. BP921414 - Show BP921414... Clone id YMU001_000149_E11 Library YMU01 Length 396 Definition Adiantum capillus-vener...is mRNA. clone: YMU001_000149_E11. Accession BP921414 Tissue type prothallium Developmental stage - Contig I... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921414|Adiantum capillus-ven...eris mRNA, clone: YMU001_000149_E11. (396 letters) Database: uniprot_sprot.fasta 412,525 sequences; 14

  4. AcEST: DK955177 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK955177 CL331Contig1 Show DK955177 Clone id TST39A01NGRL0022_G10 Library TST39 Length 5...94 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0022_G10. 5' end sequence. Accession DK955177... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955177|Adia...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955177

  5. AcEST: BP917787 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_D11 582 Adiantum capillus-veneris mRNA. clone: YMU001_000105_D11. BP917787 - Show BP9177...is mRNA. clone: YMU001_000105_D11. Accession BP917787 Tissue type prothallium Developmental stage - Contig I...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917787|Adiantum capillus-veneris mRNA,...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177

  6. AcEST: BP911772 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000009_A06 326 Adiantum capillus-veneris mRNA. clone: YMU001_000009_A06. BP911772 - Show BP91177...is mRNA. clone: YMU001_000009_A06. Accession BP911772 Tissue type prothallium Developmental stage - Contig I...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91177...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91177

  7. AcEST: BP917741 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_H02 445 Adiantum capillus-veneris mRNA. clone: YMU001_000104_H02. BP917741 - Show BP9177...is mRNA. clone: YMU001_000104_H02. Accession BP917741 Tissue type prothallium Developmental stage - Contig I... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177

  8. AcEST: BP917740 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_G12 396 Adiantum capillus-veneris mRNA. clone: YMU001_000104_G12. BP917740 - Show BP9177...is mRNA. clone: YMU001_000104_G12. Accession BP917740 Tissue type prothallium Developmental stage - Contig I...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917740|Adiantum capillus-veneris mR...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917740|Adiantum ...: 4 DLLKAYVTEVDQRDQWKNHSPLVEYVYNYSTHTSTRKTLFKVTEERLKIRLIVKTLG--K 177 D+L+A V +D +

  9. AcEST: BP917700 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_D02 544 Adiantum capillus-veneris mRNA. clone: YMU001_000104_D02. BP9177...00 CL272Contig1 Show BP917700 Clone id YMU001_000104_D02 Library YMU01 Length 544 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000104_D02. Accession BP917700 Tissue type prothallium Developmental stage... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917700|Adiantum capillus-ven...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917700|Adiantum capillus-vener

  10. AcEST: BP917775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_C08 544 Adiantum capillus-veneris mRNA. clone: YMU001_000105_C08. BP917775 - Show BP9177...is mRNA. clone: YMU001_000105_C08. Accession BP917775 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917775|Adiantum capill

  11. AcEST: BP917780 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_D03 505 Adiantum capillus-veneris mRNA. clone: YMU001_000105_D03. BP917780 - Show BP9177...is mRNA. clone: YMU001_000105_D03. Accession BP917780 Tissue type prothallium Developmental stage - Contig I...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917780|Ad...) Frame = +1 Query: 229 TLGN*FDLFGPPIRRLPF-WIVLWSILGLA*GGAC 330 TLG D+F P ++LP W+ LWS LG GG C Sbjct: 177

  12. AcEST: BP911775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000009_A09 285 Adiantum capillus-veneris mRNA. clone: YMU001_000009_A09. BP91177...5 CL2064Contig1 Show BP911775 Clone id YMU001_000009_A09 Library YMU01 Length 285 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000009_A09. Accession BP911775 Tissue type prothallium Developmental stag...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911775...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911775|Adiantum capillus

  13. AcEST: BP917799 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_F02 443 Adiantum capillus-veneris mRNA. clone: YMU001_000105_F02. BP917799 - Show BP9177...is mRNA. clone: YMU001_000105_F02. Accession BP917799 Tissue type prothallium Developmental stage - Contig I...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91779...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917799|Adiantum capillus

  14. AcEST: DK951177 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK951177 CL490Contig1 Show DK951177 Clone id TST38A01NGRL0010_L06 Library TST38 Length 6...29 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_L06. 5' end sequence. Accession DK951177...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951177|Adiantum capillus-vene...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951177|Adiantum capillus-veneris m

  15. AcEST: BP917785 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_D09 516 Adiantum capillus-veneris mRNA. clone: YMU001_000105_D09. BP917785 - Show BP9177...is mRNA. clone: YMU001_000105_D09. Accession BP917785 Tissue type prothallium Developmental stage - Contig I...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917785|Adiantum capillus-veneris mRNA,

  16. AcEST: BP916177 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000084_C01 435 Adiantum capillus-veneris mRNA. clone: YMU001_000084_C01. BP916177 - Show BP916177...is mRNA. clone: YMU001_000084_C01. Accession BP916177 Tissue type prothallium Developmental stage - Contig I...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916177...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916177

  17. AcEST: BP917716 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_E09 452 Adiantum capillus-veneris mRNA. clone: YMU001_000104_E09. BP9177...16 CL2904Contig1 Show BP917716 Clone id YMU001_000104_E09 Library YMU01 Length 452 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000104_E09. Accession BP917716 Tissue type prothallium Developmental stag...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917716|Adiant...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177

  18. AcEST: BP917771 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_C03 433 Adiantum capillus-veneris mRNA. clone: YMU001_000105_C03. BP917771 - Show BP9177...is mRNA. clone: YMU001_000105_C03. Accession BP917771 Tissue type prothallium Developmental stage - Contig I...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177

  19. AcEST: DK948177 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK948177 CL759Contig1 Show DK948177 Clone id TST38A01NGRL0002_K01 Library TST38 Length 6...19 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0002_K01. 5' end sequence. Accession DK948177...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948177|Adiantu...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948177

  20. AcEST: BP920125 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_C12 422 Adiantum capillus-veneris mRNA. clone: YMU001_000133_C12. BP920125 - Show BP92012...is mRNA. clone: YMU001_000133_C12. Accession BP920125 Tissue type prothallium Developmental stage - Contig I...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92012...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92012

  1. AcEST: BP920120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_C05 434 Adiantum capillus-veneris mRNA. clone: YMU001_000133_C05. BP920120 - Show BP92012...is mRNA. clone: YMU001_000133_C05. Accession BP920120 Tissue type prothallium Developmental stage - Contig I...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920120|Ad...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920120|

  2. AcEST: BP920127 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_D02 541 Adiantum capillus-veneris mRNA. clone: YMU001_000133_D02. BP92012...7 CL3843Contig1 Show BP920127 Clone id YMU001_000133_D02 Library YMU01 Length 541 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000133_D02. Accession BP920127 Tissue type prothallium Developmental stag...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920127|Adiantum capillus-ve... database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920127|Adian

  3. AcEST: BP920157 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G01 550 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G01. BP92015...7 CL541Contig1 Show BP920157 Clone id YMU001_000133_G01 Library YMU01 Length 550 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000133_G01. Accession BP920157 Tissue type prothallium Developmental stage...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920157|Adiantum capillus-veneris mRNA,...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920157|Adiantum capillus-veneris

  4. AcEST: BP920159 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G03 420 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G03. BP920159 - Show BP92015...is mRNA. clone: YMU001_000133_G03. Accession BP920159 Tissue type prothallium Developmental stage - Contig I... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92015... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92015

  5. AcEST: BP920156 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_F12 587 Adiantum capillus-veneris mRNA. clone: YMU001_000133_F12. BP92015...6 CL200Contig1 Show BP920156 Clone id YMU001_000133_F12 Library YMU01 Length 587 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000133_F12. Accession BP920156 Tissue type prothallium Developmental stage...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920156...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920156|Adiantum

  6. AcEST: BP919843 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000129_G09 473 Adiantum capillus-veneris mRNA. clone: YMU001_000129_G09. BP91984...3 CL2697Contig1 Show BP919843 Clone id YMU001_000129_G09 Library YMU01 Length 473 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000129_G09. Accession BP919843 Tissue type prothallium Developmental stag...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919843|Ad...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919843|Adiantum capillus-veneris

  7. AcEST: BP911984 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000011_F07 566 Adiantum capillus-veneris mRNA. clone: YMU001_000011_F07. BP911984... CL2332Contig1 Show BP911984 Clone id YMU001_000011_F07 Library YMU01 Length 566 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000011_F07. Accession BP911984 Tissue type prothallium Developmental stag...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911984|Adiantum capillus-veneris mR...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911984

  8. AcEST: BP918100 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_F01 496 Adiantum capillus-veneris mRNA. clone: YMU001_000109_F01. BP918100... CL8Contig1 Show BP918100 Clone id YMU001_000109_F01 Library YMU01 Length 496 Definition Adiantum capil...lus-veneris mRNA. clone: YMU001_000109_F01. Accession BP918100 Tissue type prothallium Developmental stage -...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918100... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918100|Adia

  9. AcEST: DK951001 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK951001 - Show DK951001 Clone id TST38A01NGRL0010_D15 Library TST38 Length 665 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_D15. 5' end sequence. Accession DK951001 Tissue t... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95100...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951001|Adiantum capillus-vener...h = 224 Score = 174 bits (442), Expect = 3e-42 Identities = 100/192 (52%), Positi

  10. AcEST: BP914062 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_D10 340 Adiantum capillus-veneris mRNA. clone: YMU001_000039_D10. BP914062 - Show BP91406...is mRNA. clone: YMU001_000039_D10. Accession BP914062 Tissue type prothallium Developmental stage - Contig I...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91406...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91406

  11. AcEST: BP914064 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_D12 560 Adiantum capillus-veneris mRNA. clone: YMU001_000039_D12. BP91406...4 CL532Contig1 Show BP914064 Clone id YMU001_000039_D12 Library YMU01 Length 560 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000039_D12. Accession BP914064 Tissue type prothallium Developmental stage...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914064|Adiantum capillus-vener...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914064|Adi

  12. AcEST: BP912406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000018_F09 348 Adiantum capillus-veneris mRNA. clone: YMU001_000018_F09. BP912406... CL1894Contig1 Show BP912406 Clone id YMU001_000018_F09 Library YMU01 Length 348 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000018_F09. Accession BP912406 Tissue type prothallium Developmental stag...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912406|Adiantum capillus-veneris mRNA...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912406

  13. AcEST: BP914067 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_E03 508 Adiantum capillus-veneris mRNA. clone: YMU001_000039_E03. BP91406...7 CL855Contig1 Show BP914067 Clone id YMU001_000039_E03 Library YMU01 Length 508 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000039_E03. Accession BP914067 Tissue type prothallium Developmental stage...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91406...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914067|Adiantum capillus-veneris mRNA, c

  14. AcEST: BP914061 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_D09 599 Adiantum capillus-veneris mRNA. clone: YMU001_000039_D09. BP91406...1 CL1730Contig1 Show BP914061 Clone id YMU001_000039_D09 Library YMU01 Length 599 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000039_D09. Accession BP914061 Tissue type prothallium Developmental stag... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914061|Adia...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914061|Adiantum capillus-veneris mRNA, c

  15. AcEST: DK951406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK951406 - Show DK951406 Clone id TST38A01NGRL0011_F06 Library TST38 Length 670 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_F06. 5' end sequence. Accession DK951406 Tissue t...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951406...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951406

  16. AcEST: BP914406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000058_E09 562 Adiantum capillus-veneris mRNA. clone: YMU001_000058_E09. BP914406... CL513Contig1 Show BP914406 Clone id YMU001_000058_E09 Library YMU01 Length 562 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000058_E09. Accession BP914406 Tissue type prothallium Developmental stage...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914406|Adiantum capillus...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914406

  17. AcEST: DK957406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK957406 - Show DK957406 Clone id TST39A01NGRL0028_E15 Library TST39 Length 641 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0028_E15. 5' end sequence. Accession DK957406 Tissue t...ed BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957406... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957406

  18. AcEST: DK944067 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK944067 CL1Contig2 Show DK944067 Clone id YMU02A01NGRL0004_O02 Library YMU02 Length 132... Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_O02. 5' end sequence. Accession DK944067...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94406... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944067|Adiantum capillus-veneri...YMU02A01NGRL0004_O02 132 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_O0

  19. AcEST: BP916406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000087_D01 556 Adiantum capillus-veneris mRNA. clone: YMU001_000087_D01. BP916406... CL1913Contig1 Show BP916406 Clone id YMU001_000087_D01 Library YMU01 Length 556 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000087_D01. Accession BP916406 Tissue type prothallium Developmental stag...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916406|Adiantum capill...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916406|Adiantum capillus-veneris mRNA, c

  20. AcEST: DK947406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK947406 CL1Contig3 Show DK947406 Clone id YMU02A01NGRL0015_M19 Library YMU02 Length 516... Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0015_M19. 5' end sequence. Accession DK947406...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947406...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947406

  1. AcEST: DK950406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK950406 CL45Contig1 Show DK950406 Clone id TST38A01NGRL0008_J04 Library TST38 Length 64...8 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_J04. 5' end sequence. Accession DK950406...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950406... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950406

  2. AcEST: BP912343 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000018_A05 305 Adiantum capillus-veneris mRNA. clone: YMU001_000018_A05. BP912343 - Show BP91234...is mRNA. clone: YMU001_000018_A05. Accession BP912343 Tissue type prothallium Developmental stage - Contig I... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91234...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912343|Adiantum capill

  3. AcEST: BP914367 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000058_B03 578 Adiantum capillus-veneris mRNA. clone: YMU001_000058_B03. BP914367 - Show BP914367...is mRNA. clone: YMU001_000058_B03. Accession BP914367 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914367|Adiantum ...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914367|

  4. AcEST: BP913677 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000033_A08 517 Adiantum capillus-veneris mRNA. clone: YMU001_000033_A08. BP913677 - Show BP91367...is mRNA. clone: YMU001_000033_A08. Accession BP913677 Tissue type prothallium Developmental stage - Contig I...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91367...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91367

  5. AcEST: BP913679 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000033_A11 505 Adiantum capillus-veneris mRNA. clone: YMU001_000033_A11. BP91367...9 CL11Contig1 Show BP913679 Clone id YMU001_000033_A11 Library YMU01 Length 505 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000033_A11. Accession BP913679 Tissue type prothallium Developmental stage ...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91367...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913679|Adiantum cap

  6. AcEST: DK962367 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK962367 CL65Contig1 Show DK962367 Clone id TST39A01NGRL0013_K22 Library TST39 Length 77...4 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_K22. 5' end sequence. Accession DK962367...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962367|Adiantum capillus-vene...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962367|Adian

  7. AcEST: DK953677 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK953677 - Show DK953677 Clone id TST39A01NGRL0018_G14 Library TST39 Length 523 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G14. 5' end sequence. Accession DK953677 Tissue t... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953677|Adiant...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95367...TST39A01NGRL0018_G14 523 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G1

  8. AcEST: DK958775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK958775 CL3350Contig1 Show DK958775 Clone id TST39A01NGRL0002_O09 Library TST39 Length ...633 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0002_O09. 5' end sequence. Accession DK958775...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK958775...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:

  9. AcEST: DK956775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK956775 CL16Contig1 Show DK956775 Clone id TST39A01NGRL0026_J23 Library TST39 Length 61...9 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0026_J23. 5' end sequence. Accession DK956775...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956775... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956775|Adiantum capillus-ven

  10. AcEST: DK955775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK955775 CL123Contig1 Show DK955775 Clone id TST39A01NGRL0024_A01 Library TST39 Length 6...07 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0024_A01. 5' end sequence. Accession DK955775...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955775...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955775

  11. AcEST: DK947775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK947775 - Show DK947775 Clone id YMU02A01NGRL0016_P09 Library YMU02 Length 451 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_P09. 5' end sequence. Accession DK947775 Tissue t... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947775|Adian...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947775|Adiantum capillus-veneris mRNA,

  12. AcEST: BP913775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000035_B05 311 Adiantum capillus-veneris mRNA. clone: YMU001_000035_B05. BP913775 - Show BP913775...is mRNA. clone: YMU001_000035_B05. Accession BP913775 Tissue type prothallium Developmental stage - Contig I...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913775|Adiantum capillus...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913775

  13. AcEST: BP919804 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000129_D02 242 Adiantum capillus-veneris mRNA. clone: YMU001_000129_D02. BP91980...4 CL1786Contig1 Show BP919804 Clone id YMU001_000129_D02 Library YMU01 Length 242 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000129_D02. Accession BP919804 Tissue type prothallium Developmental stag...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919804|Adiantum capillus-veneris m...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919804|Adiantum capillus-veneris m

  14. AcEST: DK961980 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK961980 CL1000Contig1 Show DK961980 Clone id TST39A01NGRL0011_K02 Library TST39 Length ...679 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_K02. 5' end sequence. Accession DK961980...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961980...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961980|Adiantum ca...TST39A01NGRL0011_K02 679 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_K0

  15. AcEST: BP919803 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000129_D01 477 Adiantum capillus-veneris mRNA. clone: YMU001_000129_D01. BP91980...3 CL2549Contig1 Show BP919803 Clone id YMU001_000129_D01 Library YMU01 Length 477 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000129_D01. Accession BP919803 Tissue type prothallium Developmental stag...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91980...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91980

  16. AcEST: DK959101 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK959101 CL3467Contig1 Show DK959101 Clone id TST39A01NGRL0003_M10 Library TST39 Length ...684 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0003_M10. 5' end sequence. Accession DK959101...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959101...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25...:3389-3402. Query= DK959101|Adiantum capillus-veneris mRNA, clone: TST39A01NGRL0003_M10, 5' (684 letters) Da

  17. AcEST: BP919715 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000128_C11 494 Adiantum capillus-veneris mRNA. clone: YMU001_000128_C11. BP91971...5 CL3828Contig1 Show BP919715 Clone id YMU001_000128_C11 Library YMU01 Length 494 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000128_C11. Accession BP919715 Tissue type prothallium Developmental stag...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91971... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919715|Adia

  18. AcEST: BP914460 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_B08 541 Adiantum capillus-veneris mRNA. clone: YMU001_000059_B08. BP914460 - Show BP9144...is mRNA. clone: YMU001_000059_B08. Accession BP914460 Tissue type prothallium Developmental stage - Contig I...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914460|Adiantum capil...VVFASKVPNDRMSLFSIECKEQGNLHQRDEFFLKSDDANC 143 Query: 383 LLLFPMRTSHLASPFQETTSCS 448 L H P+ +CS Sbjct: 144 YLY

  19. AcEST: BP914472 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_C09 554 Adiantum capillus-veneris mRNA. clone: YMU001_000059_C09. BP9144...72 CL1425Contig1 Show BP914472 Clone id YMU001_000059_C09 Library YMU01 Length 554 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_C09. Accession BP914472 Tissue type prothallium Developmental stag...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914472|Adiantum capillus-ve...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  20. AcEST: BP914492 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_E05 529 Adiantum capillus-veneris mRNA. clone: YMU001_000059_E05. BP914492 - Show BP9144...is mRNA. clone: YMU001_000059_E05. Accession BP914492 Tissue type prothallium Developmental stage - Contig I...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914492|Adiantum capillus-ven

  1. AcEST: BP914410 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000058_F01 608 Adiantum capillus-veneris mRNA. clone: YMU001_000058_F01. BP914410 - Show BP9144...is mRNA. clone: YMU001_000058_F01. Accession BP914410 Tissue type prothallium Developmental stage - Contig I...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  2. AcEST: BP921144 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000146_C05 517 Adiantum capillus-veneris mRNA. clone: YMU001_000146_C05. BP921144 - Show BP921144...is mRNA. clone: YMU001_000146_C05. Accession BP921144 Tissue type prothallium Developmental stage - Contig I...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921144... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921144|Adiant

  3. AcEST: DK953144 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK953144 - Show DK953144 Clone id TST38A01NGRL0015_P24 Library TST38 Length 577 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0015_P24. 5' end sequence. Accession DK953144 Tissue t...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953144...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953144

  4. AcEST: BP914463 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_B11 587 Adiantum capillus-veneris mRNA. clone: YMU001_000059_B11. BP9144...63 CL3104Contig1 Show BP914463 Clone id YMU001_000059_B11 Library YMU01 Length 587 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_B11. Accession BP914463 Tissue type prothallium Developmental stag...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914463|Adiantu...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914463|Adiantum capillus-veneris

  5. AcEST: BP914450 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_A10 499 Adiantum capillus-veneris mRNA. clone: YMU001_000059_A10. BP9144...50 CL3143Contig1 Show BP914450 Clone id YMU001_000059_A10 Library YMU01 Length 499 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_A10. Accession BP914450 Tissue type prothallium Developmental stag...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  6. AcEST: BP914476 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_D01 391 Adiantum capillus-veneris mRNA. clone: YMU001_000059_D01. BP9144...76 CL2162Contig1 Show BP914476 Clone id YMU001_000059_D01 Library YMU01 Length 391 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_D01. Accession BP914476 Tissue type prothallium Developmental stag...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914476|Adiantu...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914476

  7. AcEST: BP915144 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000067_A07 184 Adiantum capillus-veneris mRNA. clone: YMU001_000067_A07. BP915144... CL2423Contig1 Show BP915144 Clone id YMU001_000067_A07 Library YMU01 Length 184 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000067_A07. Accession BP915144 Tissue type prothallium Developmental stag...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915144...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915144|Adiantum capillus

  8. AcEST: BP911440 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000005_A06 228 Adiantum capillus-veneris mRNA. clone: YMU001_000005_A06. BP911440 - Show BP91144...is mRNA. clone: YMU001_000005_A06. Accession BP911440 Tissue type prothallium Developmental stage - Contig I...ed BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91144...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91144

  9. AcEST: BP914462 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_B10 524 Adiantum capillus-veneris mRNA. clone: YMU001_000059_B10. BP914462 - Show BP9144...is mRNA. clone: YMU001_000059_B10. Accession BP914462 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  10. AcEST: BP914456 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_B04 299 Adiantum capillus-veneris mRNA. clone: YMU001_000059_B04. BP9144...56 CL787Contig1 Show BP914456 Clone id YMU001_000059_B04 Library YMU01 Length 299 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000059_B04. Accession BP914456 Tissue type prothallium Developmental stage... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914456|Adiantum capillus-ven

  11. AcEST: BP914444 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_A03 493 Adiantum capillus-veneris mRNA. clone: YMU001_000059_A03. BP9144...44 CL1628Contig1 Show BP914444 Clone id YMU001_000059_A03 Library YMU01 Length 493 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_A03. Accession BP914444 Tissue type prothallium Developmental stag...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914444|Adiantum capillus-veneri

  12. AcEST: BP913144 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000027_A03 161 Adiantum capillus-veneris mRNA. clone: YMU001_000027_A03. BP913144... CL619Contig1 Show BP913144 Clone id YMU001_000027_A03 Library YMU01 Length 161 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000027_A03. Accession BP913144 Tissue type prothallium Developmental stage...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913144|Adiantum capillus-v...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25

  13. AcEST: BP914488 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_E01 492 Adiantum capillus-veneris mRNA. clone: YMU001_000059_E01. BP9144...88 CL2008Contig1 Show BP914488 Clone id YMU001_000059_E01 Library YMU01 Length 492 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_E01. Accession BP914488 Tissue type prothallium Developmental stag... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914488|Ad

  14. AcEST: BP914487 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_D12 521 Adiantum capillus-veneris mRNA. clone: YMU001_000059_D12. BP914487 - Show BP9144...is mRNA. clone: YMU001_000059_D12. Accession BP914487 Tissue type prothallium Developmental stage - Contig I...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914487|...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  15. AcEST: BP914443 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_A02 529 Adiantum capillus-veneris mRNA. clone: YMU001_000059_A02. BP9144...43 CL1901Contig1 Show BP914443 Clone id YMU001_000059_A02 Library YMU01 Length 529 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_A02. Accession BP914443 Tissue type prothallium Developmental stag...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  16. AcEST: BP912328 [AcEST

    Lifescience Database Archive (English)

    Full Text Available (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res...BLAST and PSI-BLAST: a new generation of protein database search programs, Nuclei...-VLHATLLV*GC 187 D G DGAH A + R A V P +IG D ICD +L T L GC Sbjct: 109 DHAHGDSADGAHRAMSPARRPLARVEPIRIGDDCWICDG...ery: 41 DIMRGLHVDGAHEARNCC*RQCASVGPTKIGVDDVICD---VLHATLLV*GC 187 D G DGAH A + R A V P +IG D ICD +L T L GC Sbjct: 109 DHAHGDSADGAHRAMS...ARNCC*RQCASVGPTKIGVDDVICD---VLHATLLV*GC 187 D G DGAH A + R A V P +IG D ICD +L T L GC Sbjct: 109 DHAHGDSADGAHRAMS

  17. AcEST: BP912593 [AcEST

    Lifescience Database Archive (English)

    Full Text Available an (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...ITHINTYRKNLKYLPTYHLPDNIYATSNIDEVLSDNNT--CIILTIPT 81 Query: 357 RAMSLFTCQILH 392 + + QI H Sbjct: 82 QQLRTICTQ...DNIYATSNIDEVLSDNNT--CIILTVPT 81 Query: 357 RAMSLFTCQILH 392 + + QI H Sbjct: 82 QQLRTICTQIQH 93 >sp|O94085|YL...++ L P+ H P + +T +D + S NT C L Sbjct: 24 ISVNLWGRDHRNTTHINTYRKNLKYLPTYHLPDNIYATSNIDEVLSDNNT--CIILTIPT 81 Query: 357 RAMS... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912593|Adiantum c

  18. AcEST: DK952432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK952432 CL2129Contig1 Show DK952432 Clone id TST38A01NGRL0014_B06 Library TST38 Length ...644 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_B06. 5' end sequence. Accession DK952432...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952432|Adi...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952432

  19. AcEST: BP914326 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F07 599 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F07. BP91432...6 CL274Contig1 Show BP914326 Clone id YMU001_000057_F07 Library YMU01 Length 599 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000057_F07. Accession BP914326 Tissue type prothallium Developmental stage...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914326...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432

  20. AcEST: BP914328 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F09 454 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F09. BP914328 - Show BP91432...is mRNA. clone: YMU001_000057_F09. Accession BP914328 Tissue type prothallium Developmental stage - Contig I...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432

  1. AcEST: DK945432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK945432 CL122Contig1 Show DK945432 Clone id YMU02A01NGRL0009_E18 Library YMU02 Length 2...39 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0009_E18. 5' end sequence. Accession DK945432...BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945432...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945432|Adiantum capillu

  2. AcEST: DK950432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK950432 CL4Contig1 Show DK950432 Clone id TST38A01NGRL0008_K07 Library TST38 Length 613... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_K07. 5' end sequence. Accession DK950432...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950432|Adiantum capillus-veneris ...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950432

  3. AcEST: BP918432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000113_D10 502 Adiantum capillus-veneris mRNA. clone: YMU001_000113_D10. BP918432... CL2516Contig1 Show BP918432 Clone id YMU001_000113_D10 Library YMU01 Length 502 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000113_D10. Accession BP918432 Tissue type prothallium Developmental stag...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918432...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918432|

  4. AcEST: DK961432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK961432 - Show DK961432 Clone id TST39A01NGRL0010_B17 Library TST39 Length 697 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_B17. 5' end sequence. Accession DK961432 Tissue t... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961432|Adian...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961432

  5. AcEST: DK946432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK946432 - Show DK946432 Clone id YMU02A01NGRL0012_J22 Library YMU02 Length 267 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0012_J22. 5' end sequence. Accession DK946432 Tissue t...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946432...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946432|Adiantum

  6. AcEST: DK959432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK959432 - Show DK959432 Clone id TST39A01NGRL0004_K19 Library TST39 Length 659 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_K19. 5' end sequence. Accession DK959432 Tissue t...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959432|Adi...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959432|A...H S IF +VG D LY++SEA+DVV+ Sbjct: 374 -KAEVSESPGERSTAQAQSEKGLEIIEVYKSSIHNSAIFASVGEDKGNLYTASEATDVVF 432 Query:

  7. AcEST: BP921521 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000150_H10 495 Adiantum capillus-veneris mRNA. clone: YMU001_000150_H10. BP921521... CL1722Contig1 Show BP921521 Clone id YMU001_000150_H10 Library YMU01 Length 495 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000150_H10. Accession BP921521 Tissue type prothallium Developmental stag... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921521...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921521|Adia

  8. AcEST: BP921219 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000147_B05 600 Adiantum capillus-veneris mRNA. clone: YMU001_000147_B05. BP92121...9 CL3191Contig1 Show BP921219 Clone id YMU001_000147_B05 Library YMU01 Length 600 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000147_B05. Accession BP921219 Tissue type prothallium Developmental stag...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921219|Adiantum capillus-...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92121

  9. AcEST: BP915919 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_E03 400 Adiantum capillus-veneris mRNA. clone: YMU001_000079_E03. BP91591...9 CL3486Contig1 Show BP915919 Clone id YMU001_000079_E03 Library YMU01 Length 400 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000079_E03. Accession BP915919 Tissue type prothallium Developmental stag...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915919|Adiant

  10. AcEST: BP915913 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_D08 427 Adiantum capillus-veneris mRNA. clone: YMU001_000079_D08. BP915913 - Show BP91591...is mRNA. clone: YMU001_000079_D08. Accession BP915913 Tissue type prothallium Developmental stage - Contig I...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591

  11. AcEST: BP915912 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_D07 482 Adiantum capillus-veneris mRNA. clone: YMU001_000079_D07. BP915912 - Show BP91591...is mRNA. clone: YMU001_000079_D07. Accession BP915912 Tissue type prothallium Developmental stage - Contig I...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915912|Adiantum capillus-veneris mR...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2...5:3389-3402. Query= BP915912|Adiantum capillus-veneris mRNA, clone: YMU001_000079_D07. (482 letters) Databas

  12. AcEST: BP911591 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000006_G06 296 Adiantum capillus-veneris mRNA. clone: YMU001_000006_G06. BP911591 - Show BP911591...is mRNA. clone: YMU001_000006_G06. Accession BP911591 Tissue type prothallium Developmental stage - Contig I...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911591|Adiantum capil...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911591|Adiantum capillus-vene

  13. AcEST: BP919213 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000122_E06 293 Adiantum capillus-veneris mRNA. clone: YMU001_000122_E06. BP919213 - Show BP91921...is mRNA. clone: YMU001_000122_E06. Accession BP919213 Tissue type prothallium Developmental stage - Contig I...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919213|Adiantu...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91921

  14. AcEST: DK954220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK954220 CL3312Contig1 Show DK954220 Clone id TST39A01NGRL0019_N21 Library TST39 Length ...597 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_N21. 5' end sequence. Accession DK954220...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954220|Adiantum capillus-... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954220

  15. AcEST: BP912220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000016_E08 357 Adiantum capillus-veneris mRNA. clone: YMU001_000016_E08. BP912220 - Show BP912220...is mRNA. clone: YMU001_000016_E08. Accession BP912220 Tissue type prothallium Developmental stage - Contig I...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912220...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912220

  16. AcEST: DK959220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK959220 CL2407Contig1 Show DK959220 Clone id TST39A01NGRL0004_B16 Library TST39 Length ...668 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_B16. 5' end sequence. Accession DK959220... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959220...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959220

  17. AcEST: BP917220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000097_G09 125 Adiantum capillus-veneris mRNA. clone: YMU001_000097_G09. BP917220 - Show BP917220...is mRNA. clone: YMU001_000097_G09. Accession BP917220 Tissue type prothallium Developmental stage - Contig I...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917220...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917220|Adiantum capillus-veneris

  18. AcEST: BP912207 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000016_D04 249 Adiantum capillus-veneris mRNA. clone: YMU001_000016_D04. BP912207 - Show BP91220...is mRNA. clone: YMU001_000016_D04. Accession BP912207 Tissue type prothallium Developmental stage - Contig I...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91220...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91220

  19. AcEST: DK946220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK946220 - Show DK946220 Clone id YMU02A01NGRL0011_N24 Library YMU02 Length 518 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0011_N24. 5' end sequence. Accession DK946220 Tissue t...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946220... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946220

  20. AcEST: BP920041 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_D04 403 Adiantum capillus-veneris mRNA. clone: YMU001_000132_D04. BP920041 - Show BP92004...is mRNA. clone: YMU001_000132_D04. Accession BP920041 Tissue type prothallium Developmental stage - Contig I...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920041|Adiantum capillus

  1. AcEST: BP920048 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_D11 495 Adiantum capillus-veneris mRNA. clone: YMU001_000132_D11. BP920048 - Show BP92004...is mRNA. clone: YMU001_000132_D11. Accession BP920048 Tissue type prothallium Developmental stage - Contig I...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920048|Adia

  2. AcEST: BP920042 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_D05 442 Adiantum capillus-veneris mRNA. clone: YMU001_000132_D05. BP920042 - Show BP92004...is mRNA. clone: YMU001_000132_D05. Accession BP920042 Tissue type prothallium Developmental stage - Contig I...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9200

  3. AcEST: BP920043 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_D06 452 Adiantum capillus-veneris mRNA. clone: YMU001_000132_D06. BP920043 - Show BP92004...is mRNA. clone: YMU001_000132_D06. Accession BP920043 Tissue type prothallium Developmental stage - Contig I..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004...25:3389-3402. Query= BP920043|Adiantum capillus-veneris mRNA, clone: YMU001_00013...1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res.

  4. Un ami qui est toujours

    Institute of Scientific and Technical Information of China (English)

    王艳

    2012-01-01

    Un jour,pendant la récréation,je suis descendue dans le bureau du secrétariat.Quand je préparais un café,une femme professeur d'espagnol s'est exclamée:《Tiens! Tu es de bonne humeur ce matin ! 》 (E)tonnée,je lui ai demandé pourquoi.Elle a répondu:《 Parce que tu prends un ca fé.》Quelle réponse inattendue! Ce n'est qu'un café instantané,un peu fade,pas très bon,à dire vrai.Si j'en prends une petite tasse,ce n'est pas que je suis de bonne humeur.C'est plut(o)t mon habitude,ou bien c'est que le café me donne une belle humeur.

  5. 微波法提取菊芋中菊糖的工艺研究%Microwave Extraction of Inulin from Jerusalem artichoke

    Institute of Scientific and Technical Information of China (English)

    贾若凌

    2012-01-01

    [ Objective] To optimize the microwave extraction technology of inulin from J. artichoke. [ Method] With fresh J. artichoke tuber as experimental materials, based on a single factor experiment, five factors of microwave power, extraction time, solid - liquid ratio, extraction frequency and extraction temperature were selected to perform an orthogonal experiment L16(45) at four levels to study the best extraction conditions of J. artichoke. [ Result] The best extraction conditions were 8 min of microwave time, 95 ℃ of extraction temperature, 500 W of microwave power, 1: 20( W/V,g/ml) of solid-liquid ratio and three times of extraction frequency; under the best conditions, the extraction rate of inulin could reach up to 68.11%. [Conclusion] The method optimized the microwave extraction conditions of inulin, and the results were accurate and practical.%[目的]优选菊芋中菊糖的微波提取工艺.[方法]以新鲜的菊芋块茎为原料,在单因素试验的基础上,以微波功率、提取时间、料液比、提取次数和提取温度为因素,设计5因素4水平的正交试验L16 (45),研究菊糖的提取及其分离纯化的最佳工艺条件.[结果]确定微波法提取菊糖的最佳工艺条件为:微波时间8 min,提取温度95℃,微波功率500W,料液比为1∶20(W/V,g/ml,下同),提取3次;在此条件下,菊芋葡糖的提取率为68.11%.[结论]该方法优选出了菊芋中菊糖的微波提取工艺,结果准确可行.

  6. 菊芋中菊糖提取与纯化工艺研究%Study on Extraction and Purification Technology of Inulin from Jerusalem Artichokes

    Institute of Scientific and Technical Information of China (English)

    王振强; 申森; 张耀光

    2011-01-01

    Study that the extraction and purification of jerusalem artichoke. First, the drying method of the artichoke slices that was being dried in the oven by hot air at 60 ℃ was indicated. And the extraction temperature, the extraction time, the solid to liquid ratio, the extraction times were selected for the singlefactor test. Second, the range of conditions was determined, the extraction conditions were optimized by orthogonal experiment, the optimal conditions of the inulin extraction from jerusalem artichoke were obtained, the extraction temperature was 70 ℃, the extraction time was 70 min, the solid to liquid ratio was 1 : 30, the extraction times was two, and the inulin yield rate was up to 52.80%. Others, the best treatment conditions of protein removal by milk of lime were determined, water bath temperature was between 70 ℃ and 80 ℃, water bath time was from 60 min to 90 min, with the best effect of depigmentation by macroporous adsorptive resins S-8 and the low rate of inulin loss.%研究了菊芋中菊糖的提取与纯化,确定了菊芋片干燥方法为60℃烘箱热风烘干。选择提取温度、提取时间、料液比、提取次数进行单因素试验,确定条件范围,再采用正交试验优化提取条件,得到菊芋中菊糖提取最佳工艺条件为温度70℃,提取时间70min,料液比1:30,提取次数2次,菊糖得率可达52.80%。并确定了石灰乳法脱蛋白最佳处理条件为水浴温度70℃~80℃,水浴时间60min-90min,大孔吸附树脂S.8的脱色素效果最好,菊糖损失率较低。

  7. Compressing DNA sequence databases with coil

    Directory of Open Access Journals (Sweden)

    Hendy Michael D

    2008-05-01

    Full Text Available Abstract Background Publicly available DNA sequence databases such as GenBank are large, and are growing at an exponential rate. The sheer volume of data being dealt with presents serious storage and data communications problems. Currently, sequence data is usually kept in large "flat files," which are then compressed using standard Lempel-Ziv (gzip compression – an approach which rarely achieves good compression ratios. While much research has been done on compressing individual DNA sequences, surprisingly little has focused on the compression of entire databases of such sequences. In this study we introduce the sequence database compression software coil. Results We have designed and implemented a portable software package, coil, for compressing and decompressing DNA sequence databases based on the idea of edit-tree coding. coil is geared towards achieving high compression ratios at the expense of execution time and memory usage during compression – the compression time represents a "one-off investment" whose cost is quickly amortised if the resulting compressed file is transmitted many times. Decompression requires little memory and is extremely fast. We demonstrate a 5% improvement in compression ratio over state-of-the-art general-purpose compression tools for a large GenBank database file containing Expressed Sequence Tag (EST data. Finally, coil can efficiently encode incremental additions to a sequence database. Conclusion coil presents a compelling alternative to conventional compression of flat files for the storage and distribution of DNA sequence databases having a narrow distribution of sequence lengths, such as EST data. Increasing compression levels for databases having a wide distribution of sequence lengths is a direction for future work.

  8. AcEST: DK959005 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. ...ct: 64 KINGKPLPGATPAKRFKLNYATYG---KQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVY 120 Query: 453 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMS...AKVIIDPITNRSKGYGFVRFGDENERTRAMSEMNG-VYCSSRPMRISVA 611 PS RG KV++D T SKGYGFV+F DE ...SLFVGDLTPDVDDGMLYEFFVKVY 120 Query: 453 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMSEMNG-VYCSSRPMRISVA 611 PS RG KV+...KLNYVTYG---KQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVY 120 Query: 453 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMS

  9. Analysis of ESTs from multiple Gossypium hirsutum tissues and identification of SSRs.

    Science.gov (United States)

    Taliercio, Earl; Allen, Randy D; Essenberg, Margaret; Klueva, Natalya; Nguyen, Henry; Patil, Mohini A; Payton, Paxton; Millena, Ana Cecilia M; Phillips, Angela L; Pierce, Margaret L; Scheffler, Brian; Turley, Rickie; Wang, Jing; Zhang, Deshui; Scheffler, Jodi

    2006-04-01

    In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical analysis of a subset of these ESTs identified genes likely differentially expressed in stems, cotyledons, and drought-stressed tissues. Annotation of the differentially expressed cDNAs tentatively identified genes involved in lignin metabolism, starch biosynthesis and stress response, consistent with pathways likely to be active in the tissues under investigation. Simple sequence repeats (SSRs) were identified among these ESTs, and an inexpensive method was developed to screen genomic DNA for the presence of these SSRs. At least 69 SSRs potentially useful in mapping were identified. Selected amplified SSRs were isolated and sequenced. The sequences corresponded to the EST containing the SSRs, confirming that these SSRs will potentially map the gene represented by the EST. The ESTs containing SSRs were annotated to help identify the genes that may be mapped using these markers. PMID:16699550

  10. Cell Centred Database (CCDB)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated...

  11. Native Health Research Database

    Science.gov (United States)

    ... APP WITH JAVASCRIPT TURNED OFF. THE NATIVE HEALTH DATABASE REQUIRES JAVASCRIPT IN ORDER TO FUNCTION. PLEASE ENTER ... To learn more about searching the Native Health Database, click here. Keywords Title Author Source of Publication ...

  12. AIDSinfo Drug Database

    Science.gov (United States)

    ... Widgets Order Publications Skip Nav AIDS info Drug Database Home > Drugs Español small medium large Text Size ... health care providers and patients. Search the Drug Database Help × Search by drug name Performs a search ...

  13. Database Urban Europe

    NARCIS (Netherlands)

    Sleutjes, B.; de Valk, H.A.G.

    2016-01-01

    Database Urban Europe: ResSegr database on segregation in The Netherlands. Collaborative research on residential segregation in Europe 2014–2016 funded by JPI Urban Europe (Joint Programming Initiative Urban Europe).

  14. Scopus database: a review.

    Science.gov (United States)

    Burnham, Judy F

    2006-03-08

    The Scopus database provides access to STM journal articles and the references included in those articles, allowing the searcher to search both forward and backward in time. The database can be used for collection development as well as for research. This review provides information on the key points of the database and compares it to Web of Science. Neither database is inclusive, but complements each other. If a library can only afford one, choice must be based in institutional needs.

  15. Web database development

    OpenAIRE

    Tsardas, Nikolaos A.

    2001-01-01

    This thesis explores the concept of Web Database Development using Active Server Pages (ASP) and Java Server Pages (JSP). These are among the leading technologies in the web database development. The focus of this thesis was to analyze and compare the ASP and JSP technologies, exposing their capabilities, limitations, and differences between them. Specifically, issues related to back-end connectivity using Open Database Connectivity (ODBC) and Java Database Connectivity (JDBC), application ar...

  16. IP Geolocation Databases: Unreliable?

    OpenAIRE

    Poese, Ingmar; Uhlig, Steve; Kaafar, Mohamed Ali; Donnet, Benoît; Gueye, Bamba

    2011-01-01

    The most widely used technique for IP geolocation con- sists in building a database to keep the mapping between IP blocks and a geographic location. Several databases are available and are frequently used by many services and web sites in the Internet. Contrary to widespread belief, geolo- cation databases are far from being as reliable as they claim. In this paper, we conduct a comparison of several current geolocation databases -both commercial and free- to have an insight of the limitation...

  17. Refactoring of a Database

    OpenAIRE

    Dsousa, Ayeesha; Bhatia, Shalini

    2009-01-01

    The technique of database refactoring is all about applying disciplined and controlled techniques to change an existing database schema. The problem is to successfully create a Database Refactoring Framework for databases. This paper concentrates on the feasibility of adapting this concept to work as a generic template. To retain the constraints regardless of the modifications to the metadata, the paper proposes a MetaData Manipulation Tool to facilitate change. The tool adopts a Template Des...

  18. Scopus database: a review

    OpenAIRE

    Burnham, Judy F.

    2006-01-01

    The Scopus database provides access to STM journal articles and the references included in those articles, allowing the searcher to search both forward and backward in time. The database can be used for collection development as well as for research. This review provides information on the key points of the database and compares it to Web of Science. Neither database is inclusive, but complements each other. If a library can only afford one, choice must be based in institutional needs.

  19. A possible general mechanism for ultrasound-assisted extraction (UAE) suggested from the results of UAE of chlorogenic acid from Cynara scolymus L. (artichoke) leaves.

    Science.gov (United States)

    Saleh, I A; Vinatoru, M; Mason, T J; Abdel-Azim, N S; Aboutabl, E A; Hammouda, F M

    2016-07-01

    The use of ultrasound-assisted extraction (UAE) for the extraction of chlorogenic acid (CA) from Cynara scolymus L., (artichoke) leaves using 80% methanol at room temperature over 15 min gave a significant increase in yield (up to a 50%) compared with maceration at room temperature and close to that obtained by boiling over the same time period. A note of caution is introduced when comparing UAE with Soxhlet extraction because, in the latter case, the liquid entering the Soxhlet extractor is more concentrated in methanol (nearly 100%) that the solvent in the reservoir (80% methanol) due to fractionation during distillation. The mechanism of UAE is discussed in terms of the effects of cavitation on the swelling index, solvent diffusion and the removal of a stagnant layer of solvent surrounding the plant material. PMID:26964956

  20. Production of a single cyclic type of fructooligosaccharide structure by inulin-degrading Paenibacillus sp. LX16 newly isolated from Jerusalem artichoke root.

    Science.gov (United States)

    Yao, Zhihua; Guo, Jiqiang; Tang, Wenzhu; Sun, Zhen; Hou, Yingmin; Li, Xianzhen

    2016-05-01

    A novel inulin-degrading bacterium was isolated from a soil sample collected on Jerusalem artichoke roots. It is a Gram-positive, aerobic, motile and central endospore-forming straight rod, and exhibits phenotypic properties being consistent with its classification in the genus Paenibacillus. The predominant cellular fatty acids were anteiso-C15:0, C16:0 and anteiso-C17:0. This strain represents a novel species of the genus Paenibacillus on the basis of phenotypic data together with phylogenetic analysis, and it is here designated as LX16 and deposited in China centre for type collection, China (= CCTCC 2015256). Strain LX16 could produce a cyclofructooligosaccharide fructanotransferase catalysing the formation of one type of fructooligosaccharide (FOS) from inulin. The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6. Such homology in inulin degradation products may be beneficial for the functional FOS production.

  1. 酒曲应用于菊芋发酵的初步研究%A preliminary study fermentation of Jerusalem artichoke with koji

    Institute of Scientific and Technical Information of China (English)

    冯迪; 隆小华; 辛本荣; 刘兆普

    2011-01-01

    Jerusalem artichoke is a kind of alternative ethanol fermentation of raw materials. Compared with starch materials,the temperature of paste for Jerusalem artichoke is lower,which has the advantage in saving energy.Anqi liquor-making koji, Anqi Liqueur Koji and Xinma Liquor Industry Koji were used to study by one-step fermentation experiment and demonstrate the feasibility of producing ethanol fermentation of Jerusalem artichoke.The results were as follows:In the low raw sugar concentration and general treated conditions ,the alcohol content of Anqi liquor-making koji was higher than the other two koji, up to 5.5 degrees. Fermentation process, the weight loss of carbon dioxide of Anqi liquor-making koji was always higher than the other two koji. Use Anqi liquor-making koji to ferment,the ethanol concentration increased with the experimental time lasting during three days. With the further extension of the fermentation time, pH value of its broth was 6.35. pH value fell to 4.82 after 1 day fermentation and 4.48 after 3 days later,then pH value of the trend was stable. It can be concluded that compared with the other two koji, Anqi liquor-making koji was more suitable for bioethanol production using Jerusalem artichoke.%菊芋是一种极具潜力的乙醇发酵原料,与淀粉质原料相比,菊芋低温条件下即可实现糊化,在节能方面具有优势.本文选取了安琪甜酒曲、安琪酿酒曲和欣马酒业酒曲进行一步法发酵实验,论证不同种类及品种的酒曲应用于菊芋发酵生产乙醇的可行性.结果表明:在原料含糖量不高且未经任何条件优化的情况下,安琪酿酒曲的酒度高于其他两种酒曲,酒度可达5.5度;发酵过程中,安琪酿酒曲的CO2失重量始终高于安琪甜酒曲和欣马酒曲;使用安琪酿酒曲进行发酵,第1d到第3d乙醇含量逐渐升高,在第3d时达到最大值;随着发酵时间的继续延长,安琪酿酒曲发酵液PH为6.35.发酵1d后pH降至4.82,3d后pH降至4

  2. Automated Oracle database testing

    CERN Document Server

    CERN. Geneva

    2014-01-01

    Ensuring database stability and steady performance in the modern world of agile computing is a major challenge. Various changes happening at any level of the computing infrastructure: OS parameters & packages, kernel versions, database parameters & patches, or even schema changes, all can potentially harm production services. This presentation shows how an automatic and regular testing of Oracle databases can be achieved in such agile environment.

  3. The Effect of Ginger (Zingiber officinalis and Artichoke (Cynara cardunculus Extract Supplementation on Functional Dyspepsia: A Randomised, Double-Blind, and Placebo-Controlled Clinical Trial

    Directory of Open Access Journals (Sweden)

    Attilio Giacosa

    2015-01-01

    Full Text Available Objective. Functional dyspepsia (FD is a frequent clinical finding in western world. The aim of this study is to compare the efficacy of a ginger and artichoke supplementation versus placebo in the treatment of FD. Methods. A prospective multicentre, double blind, randomized, placebo controlled, parallel-group comparison of the supplement and placebo over a period of 4 weeks was performed. Two capsules/day were supplied (before lunch and dinner to 126 FD patients (supplementation/placebo: 65/61. Results. After 14 days of treatment, only supplementation group (SG showed a significant amelioration (SG: αS=+1.195 MCA score units (u, P=0.017; placebo: αP=+0.347 u, P=0.513. The intercept (α resulted to be significantly higher in SG than in placebo (αS-αP=+0.848 u, P<0.001. At the end of the study, the advantage of SG versus placebo persists without variation (βS-βP=+0.077 u, P=0.542. In SG, a significant advantage is observed for nausea (βS-βP=-0.398 u, P<0.001, epigastric fullness (βS-βP=-0.241, P<0.001, epigastric pain (βS-βP=-0.173 u, P=0.002, and bloating (βS-βP=-0.167 u, P=0.017. Conclusions. The association between ginger and artichoke leaf extracts appears safe and efficacious in the treatment of FD and could represent a promising treatment for this disease.

  4. The wild Egyptian artichoke as a promising functional food for the treatment of hepatitis C virus as revealed via UPLC-MS and clinical trials.

    Science.gov (United States)

    Elsebai, Mahmoud Fahmi; Abass, Khaled; Hakkola, Jukka; Atawia, Ahmed Rezk; Farag, Mohamed A

    2016-07-13

    Infection by hepatitis C virus (HCV) and its subsequent complications are a major cause of mortality worldwide. The water extract of the wild Egyptian artichoke (WEA) (Cynara cardunculus L. var. sylvestris (Lam.) Fiori) leaves is a freely available herbal product that is used for treatment of HCV-infection complications such as jaundice and ascites. The purpose of this study was to evaluate whether WEA exhibits activity against HCV, identify bioactive chemicals in its extract and to tentatively examine the potential inhibitory interactions of WEA with human drug-metabolizing enzymes. The current pilot clinical trial revealed that the water extract of a WEA plant decreased the HCV viral load below the detection level in 12 out of 15 patients. Furthermore, the liver enzymes ALT and AST, as well as the level of bilirubin were normalized. The total WEA extract inhibited CYP2B6 (OH-BUP) and CYP2C19 (5-OH-OME) with high affinity, IC50 ∼ 20 μg ml(-1), while moderate inhibitory interactions were observed for CYP1A2, CYP2D6, CYP2E1 and CYP3A4. Results presented herein suggest that the WEA exhibits strong antiviral activity against HCV and may be useful for its treatment. Compared to the artichoke product "Hepar SL Forte(®)", WEA was found to be more enriched in sesquiterpenes versus the abundance of phenolic compounds, especially flavonoids in Hepar SL Forte(®) as revealed via UPLC-MS analysis coupled to chemometrics. PMID:27296047

  5. CTD_DATABASE - Cascadia tsunami deposit database

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Cascadia Tsunami Deposit Database contains data on the location and sedimentological properties of tsunami deposits found along the Cascadia margin. Data have...

  6. AcEST: DK955280 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ryza sati... 86 1e-16 sp|Q25AG5|ERG3_ORYSI Elicitor-responsive protein 3 OS=Oryza sati... 86 1e-16 sp|A0FGR8|ESYT2_HUMAN Extended...6e-11 sp|Q3TZZ7|ESYT2_MOUSE Extended synaptotagmin-2 OS=Mus musculus G... 67 8e-1...ne domain-conta... 59 2e-08 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2-B OS=Xenopus laev... 59 2e-08 sp|Q5M7N9|ESYT3_XENTR Extend...ed synaptotagmin-3 OS=Xenopus tropic... 58 3e-08 sp|Q5FWL4|EST2A_XENLA Extended...MOUSE Ras GTPase-activating protein 4 OS=Mus mus... 52 2e-06 sp|A0FGR9|ESYT3_HUMAN Extended synaptotagmin-3

  7. AcEST: DK959751 [AcEST

    Lifescience Database Archive (English)

    Full Text Available .. 86 1e-16 sp|A0FGR8|ESYT2_HUMAN Extended synaptotagmin-2 OS=Homo sapiens G... 72 3e-12 sp|Q6PFQ7|RASL2_MOU...SE Ras GTPase-activating protein 4 OS=Mus mus... 72 4e-12 sp|Q3TZZ7|ESYT2_MOUSE Extended...ivating-like protein 1 OS=Mus mu... 65 3e-10 sp|Q5M7N9|ESYT3_XENTR Extended synaptotagmin-3 OS=Xenopus tropi... 1 OS=Homo s... 61 6e-09 sp|Q8L7A4|AGD11_ARATH Probable ADP-ribosylation factor GTPase-ac... 61 6e-09 sp|Q7ZWU7|EST2B_XENLA Extended... synaptotagmin-2-B OS=Xenopus laev... 60 1e-08 sp|Q5FWL4|EST2A_XENLA Extended

  8. AcEST: DK959757 [AcEST

    Lifescience Database Archive (English)

    Full Text Available hit_id Q5DTI8 Definition sp|Q5DTI8|ESYT3_MOUSE Extended synaptotagmin-3 OS=Mus musculus Align length 95 Scor... alignments: (bits) Value sp|Q5DTI8|ESYT3_MOUSE Extended synaptotagmin-3 OS=Mus m...usculus G... 56 2e-07 sp|Q5M7N9|ESYT3_XENTR Extended synaptotagmin-3 OS=Xenopus tropic... 55 5e-07 sp|A0FGR9|ESYT3_HUMAN Extended... synaptotagmin-3 OS=Homo sapiens G... 53 2e-06 sp|Q5FWL4|EST2A_XENLA Extended synaptota...gmin-2-A OS=Xenopus laev... 52 3e-06 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2-B OS=Xenopus laev... 49

  9. AcEST: DK960778 [AcEST

    Lifescience Database Archive (English)

    Full Text Available _HUMAN Myoferlin OS=Homo sapiens GN=FER1L3 PE=1 SV=1 40 0.007 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2...-B OS=Xenopus laev... 38 0.037 sp|Q5FWL4|EST2A_XENLA Extended synaptotagmin-2-A OS=Xenopus laev... 38 0.037 ...sp|Q9VVI3|NEDD4_DROME E3 ubiquitin-protein ligase Nedd-4 OS=Dros... 37 0.063 sp|Q9BSJ8|ESYT1_HUMAN Extended ...synaptotagmin-1 OS=Homo sapiens G... 37 0.063 sp|Q3U7R1|ESYT1_MOUSE Extended syna

  10. AcEST: DK955788 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Oryza sati... 67 6e-11 sp|Q3TZZ7|ESYT2_MOUSE Extended synaptotagmin-2 OS=Mus musculus G... 66 1e-10 sp|Q9ZT4...848_DICDI Probable serine/threonine-protein kinase D... 65 3e-10 sp|A0FGR8|ESYT2_HUMAN Extended synaptotagmi...p|P41823|SY65_APLCA Synaptotagmin-1 OS=Aplysia californica GN=S... 60 7e-09 sp|Q5M7N9|ESYT3_XENTR Extended s...ynaptotagmin-3 OS=Xenopus tropic... 60 7e-09 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2-B OS=Xenopus lae...v... 60 9e-09 sp|Q5FWL4|EST2A_XENLA Extended synaptotagmin-2-A OS=Xenopus laev... 60 9e-09 sp|P48231|TCB2_YE

  11. AcEST: BP912089 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_H11 578 Adiantum capillus-veneris mRNA. clone: YMU001_000012_H11. BP9120...89 CL1268Contig1 Show BP912089 Clone id YMU001_000012_H11 Library YMU01 Length 578 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_H11. Accession BP912089 Tissue type prothallium Developmental stag...BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912089|Adiantum

  12. AcEST: DK951203 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK951203 CL1117Contig1 Show DK951203 Clone id TST38A01NGRL0010_M09 Library TST38 Length ...651 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_M09. 5' end sequence. Accession DK95120...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951203|Ad... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951203|Adiant..._ARATH Uncharacterized protein At4g32590.3 OS=Ar... 120 7e-26 tr|Q7XHS1|Q7XHS1_OR

  13. AcEST: BP912029 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_C02 263 Adiantum capillus-veneris mRNA. clone: YMU001_000012_C02. BP9120...29 CL2240Contig1 Show BP912029 Clone id YMU001_000012_C02 Library YMU01 Length 263 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_C02. Accession BP912029 Tissue type prothallium Developmental stag...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912029|Adiantum capillus-veneris mRN... database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912029|Adiantum capillus-veneris mRNA,

  14. AcEST: BP912053 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_E04 538 Adiantum capillus-veneris mRNA. clone: YMU001_000012_E04. BP912053 - Show BP9120...is mRNA. clone: YMU001_000012_E04. Accession BP912053 Tissue type prothallium Developmental stage - Contig I...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...IMDELFQDR 188 >sp|A7MTT7|SURE_VIBHB 5'-nucleotidase surE OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912053|Adiantum capillus-veneris m

  15. AcEST: BP912062 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_F02 553 Adiantum capillus-veneris mRNA. clone: YMU001_000012_F02. BP9120...62 CL1860Contig1 Show BP912062 Clone id YMU001_000012_F02 Library YMU01 Length 553 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_F02. Accession BP912062 Tissue type prothallium Developmental stag...7), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  16. AcEST: BP912812 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000023_B07 575 Adiantum capillus-veneris mRNA. clone: YMU001_000023_B07. BP912812... CL2610Contig1 Show BP912812 Clone id YMU001_000023_B07 Library YMU01 Length 575 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000023_B07. Accession BP912812 Tissue type prothallium Developmental stag... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912812|Adiant...um capillus-veneris mRNA, clone: YMU001_000023_B07. (575 letters) Database: uniprot_sprot.fasta 412,525 sequ

  17. AcEST: BP912124 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D06 531 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D06. BP91212...4 CL2988Contig1 Show BP912124 Clone id YMU001_000015_D06 Library YMU01 Length 531 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000015_D06. Accession BP912124 Tissue type prothallium Developmental stag...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912124|Adiantum capillus-veneris mRNA,... clone: YMU001_000015_D06. (531 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total

  18. AcEST: BP912412 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000018_G03 551 Adiantum capillus-veneris mRNA. clone: YMU001_000018_G03. BP912412... CL4248Contig1 Show BP912412 Clone id YMU001_000018_G03 Library YMU01 Length 551 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000018_G03. Accession BP912412 Tissue type prothallium Developmental stag...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912412|Adiantum capillus-veneris mR...NA, clone: YMU001_000018_G03. (551 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 tot

  19. AcEST: BP912112 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_C08 546 Adiantum capillus-veneris mRNA. clone: YMU001_000015_C08. BP912112 - Show BP912112...is mRNA. clone: YMU001_000015_C08. Accession BP912112 Tissue type prothallium Developmental stage - Contig I...Arabidopsis thaliana Align length 171 Score (bit) 121.0 E-value 3.0e-27 Report BLASTX 2.2.19 [Nov-02-2008] R... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912112|Adiantum capillus-veneri...s mRNA, clone: YMU001_000015_C08. (546 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765

  20. AcEST: DK961212 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0009_I01 591 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_I0...1. 5' end sequence. DK961212 - Show DK961212 Clone id TST39A01NGRL0009_I01 Library TST39 Length 591 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_I01. 5' end sequence. Accession DK961212 Tissue t...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961212|Adiantum capil...lus-veneris mRNA, clone: TST39A01NGRL0009_I01, 5' (591 letters) Database: uniprot_sprot.fasta 412

  1. AcEST: BP912127 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D09 582 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D09. BP912127 - Show BP91212...is mRNA. clone: YMU001_000015_D09. Accession BP912127 Tissue type prothallium Developmental stage - Contig I...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912127|Adiantum capillus-veneris mRNA,... clone: YMU001_000015_D09. (582 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total ...p|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2 OS=Arabidopsis th... 79 2e-14 sp|Q09912|PSI1_SCHPO Protein psi

  2. AcEST: BP912120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D01 500 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D01. BP912120 - Show BP91212...is mRNA. clone: YMU001_000015_D01. Accession BP912120 Tissue type prothallium Developmental stage - Contig I...elated Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum Align length 130 Score (bit) 124.0 E-va...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91212...0|Adiantum capillus-veneris mRNA, clone: YMU001_000015_D01. (500 letters) Database: uniprot_sprot.fasta 412

  3. AcEST: DK951212 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0010_M18 620 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_M1...8. 5' end sequence. DK951212 CL3520Contig1 Show DK951212 Clone id TST38A01NGRL0010_M18 Library TST38 Length ...620 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_M18. 5' end sequence. Accession DK951212...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951212|Adiantum capillus-veneris ...mRNA, clone: TST38A01NGRL0010_M18, 5' (620 letters) Database: uniprot_sprot.fasta 412

  4. AcEST: DK962016 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK962016 - Show DK962016 Clone id TST39A01NGRL0011_L15 Library TST39 Length 642 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L15. 5' end sequence. Accession DK962016 Tissue t...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962016...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25...TST39A01NGRL0011_L15 642 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L1

  5. AcEST: DK963030 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0015_H02 630 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0015_H0...2. 5' end sequence. DK963030 - Show DK963030 Clone id TST39A01NGRL0015_H02 Library TST39 Length 630 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0015_H02. 5' end sequence. Accession DK963030 Tissue t...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK963030|Adian...tum capillus-veneris mRNA, clone: TST39A01NGRL0015_H02, 5' (630 letters) Database

  6. AcEST: BP919945 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000131_A09 566 Adiantum capillus-veneris mRNA. clone: YMU001_000131_A09. BP91994...5 CL1544Contig1 Show BP919945 Clone id YMU001_000131_A09 Library YMU01 Length 566 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000131_A09. Accession BP919945 Tissue type prothallium Developmental stag... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91994...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919945|Adiantum capillus-veneris mRN

  7. AcEST: BP914714 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000062_A12 491 Adiantum capillus-veneris mRNA. clone: YMU001_000062_A12. BP914714... CL3718Contig1 Show BP914714 Clone id YMU001_000062_A12 Library YMU01 Length 491 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000062_A12. Accession BP914714 Tissue type prothallium Developmental stag...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914714|Adiantum capillus-veneris ...mRNA, clone: YMU001_000062_A12. (491 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 t

  8. AcEST: BP914214 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000056_D04 404 Adiantum capillus-veneris mRNA. clone: YMU001_000056_D04. BP914214 - Show BP914214...is mRNA. clone: YMU001_000056_D04. Accession BP914214 Tissue type prothallium Developmental stage - Contig I... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914214|Adiantum capillus-ven...eris mRNA, clone: YMU001_000056_D04. (388 letters) Database: uniprot_sprot.fasta 412,525 sequences; 14...l cation channel ... 29 8.4 sp|Q91WD2|TRPV6_MOUSE Transient receptor potential cation channe... 29 8.4 sp|P14

  9. AcEST: BP914314 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_E07 575 Adiantum capillus-veneris mRNA. clone: YMU001_000057_E07. BP914314 - Show BP914314...is mRNA. clone: YMU001_000057_E07. Accession BP914314 Tissue type prothallium Developmental stage - Contig I...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914314|Adiantum capillus-v...eneris mRNA, clone: YMU001_000057_E07. (575 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,80...(67), Expect = 6.6 Identities = 20/72 (27%), Positives = 34/72 (47%), Gaps = 4/72 (5%) Frame = +2 Query: 149

  10. AcEST: BP914141 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000042_D04 288 Adiantum capillus-veneris mRNA. clone: YMU001_000042_D04. BP914141 - Show BP91414...is mRNA. clone: YMU001_000042_D04. Accession BP914141 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914141|Adiantum ...capillus-veneris mRNA, clone: YMU001_000042_D04. (264 letters) Database: uniprot_sprot.fasta 412,525 sequences; 14... subunit beta, chloroplastic OS... 106 4e-23 sp|P62614|ATPB_AEGCR ATP synthase subunit beta, chloroplastic O

  11. AcEST: BP914140 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000042_D03 527 Adiantum capillus-veneris mRNA. clone: YMU001_000042_D03. BP914140 - Show BP91414...is mRNA. clone: YMU001_000042_D03. Accession BP914140 Tissue type prothallium Developmental stage - Contig I...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91414...0|Adiantum capillus-veneris mRNA, clone: YMU001_000042_D03. (527 letters) Database: uniprot_sprot.fasta 412,525 sequences; 14...ein DLN-1 OS=Homo sapiens GN=C10orf140 ... 34 0.49 sp|Q6DE84|MAFB_XENLA Transcription factor MafB OS=Xenopus

  12. AcEST: DK961776 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK961776 CL125Contig1 Show DK961776 Clone id TST39A01NGRL0011_B10 Library TST39 Length 7...08 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_B10. 5' end sequence. Accession DK96177...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96177...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961776|Adiantum capillus-veneris mR...TST39A01NGRL0011_B10 708 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_B1

  13. AcEST: DK951773 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK951773 CL1Contig3 Show DK951773 Clone id TST38A01NGRL0012_E18 Library TST38 Length 446... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_E18. 5' end sequence. Accession DK951773...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95177...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951773|Adiantum capillus-v...TST38A01NGRL0012_E18 446 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_E1

  14. AcEST: BP917724 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_F05 279 Adiantum capillus-veneris mRNA. clone: YMU001_000104_F05. BP9177...24 CL1287Contig1 Show BP917724 Clone id YMU001_000104_F05 Library YMU01 Length 279 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000104_F05. Accession BP917724 Tissue type prothallium Developmental stag...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917724|Adian...7), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177

  15. AcEST: BP917761 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_B04 422 Adiantum capillus-veneris mRNA. clone: YMU001_000105_B04. BP9177...61 CL1598Contig1 Show BP917761 Clone id YMU001_000105_B04 Library YMU01 Length 422 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000105_B04. Accession BP917761 Tissue type prothallium Developmental stag...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917761|Adiantum capillus-vener

  16. AcEST: BP917731 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000104_G01 113 Adiantum capillus-veneris mRNA. clone: YMU001_000104_G01. BP9177...31 CL2673Contig1 Show BP917731 Clone id YMU001_000104_G01 Library YMU01 Length 113 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000104_G01. Accession BP917731 Tissue type prothallium Developmental stag...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917731|Adiantum capill

  17. AcEST: DK951771 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK951771 - Show DK951771 Clone id TST38A01NGRL0012_E16 Library TST38 Length 544 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_E16. 5' end sequence. Accession DK951771 Tissue t...database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951771|Adiantum capillus-veneris mRNA, c...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95177...TST38A01NGRL0012_E16 544 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_E1

  18. AcEST: DK952012 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK952012 CL572Contig1 Show DK952012 Clone id TST38A01NGRL0012_P04 Library TST38 Length 6...21 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_P04. 5' end sequence. Accession DK952012...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952012|Adiantu...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952012...TST38A01NGRL0012_P04 621 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_P0

  19. AcEST: DK962015 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK962015 CL287Contig1 Show DK962015 Clone id TST39A01NGRL0011_L14 Library TST39 Length 5...74 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L14. 5' end sequence. Accession DK962015...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962015|Adiantum capillus-v...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962015...TST39A01NGRL0011_L14 574 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L1

  20. AcEST: DK951002 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK951002 CL1Contig3 Show DK951002 Clone id TST38A01NGRL0010_D16 Library TST38 Length 619... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_D16. 5' end sequence. Accession DK951002... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951002|Adiant...1 Length = 33 Score = 62.4 bits (150), Expect = 2e-09 Identities = 33/33 (100%), Positives = 33/33 (100...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95100

  1. AcEST: DK951006 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK951006 CL11Contig1 Show DK951006 Clone id TST38A01NGRL0010_D20 Library TST38 Length 68...0 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_D20. 5' end sequence. Accession DK95100..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95100...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951006|Adiantum capillus-ve...TST38A01NGRL0010_D20 680 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_D2

  2. AcEST: DK959100 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK959100 CL301Contig1 Show DK959100 Clone id TST39A01NGRL0003_M09 Library TST39 Length 6...63 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0003_M09. 5' end sequence. Accession DK959100...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959100|...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959100|Adiantum capillus-vene..... 103 9e-21 tr|Q9FXH3|Q9FXH3_ARATH F6F9.20 protein (Putative uncharacterized... 100 1e-19 tr|Q6Z4A9|Q6Z4A9_

  3. AcEST: DK961007 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK961007 CL396Contig1 Show DK961007 Clone id TST39A01NGRL0008_P07 Library TST39 Length 6...87 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_P07. 5' end sequence. Accession DK96100...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96100...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96100...TST39A01NGRL0008_P07 687 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_P0

  4. AcEST: DK961003 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK961003 CL30Contig1 Show DK961003 Clone id TST39A01NGRL0008_P03 Library TST39 Length 69...3 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_P03. 5' end sequence. Accession DK96100...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961003|...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96100...TST39A01NGRL0008_P03 693 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_P0

  5. AcEST: DK944065 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK944065 CL1Contig3 Show DK944065 Clone id YMU02A01NGRL0004_N24 Library YMU02 Length 522... Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_N24. 5' end sequence. Accession DK944065... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944065|Adiantum capillus-veneris mRNA, ...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94406...YMU02A01NGRL0004_N24 522 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_N2

  6. AcEST: DK956406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK956406 CL111Contig1 Show DK956406 Clone id TST39A01NGRL0025_K13 Library TST39 Length 5...91 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_K13. 5' end sequence. Accession DK956406...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956406...tr... 231 2e-60 sp|P53445|ALF1_LAMJA Fructose-bisphosphate aldolase, muscle type... 229 6e-60 sp|Q40677|ALFC...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956406

  7. AcEST: DK946406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK946406 - Show DK946406 Clone id YMU02A01NGRL0012_I08 Library YMU02 Length 540 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0012_I08. 5' end sequence. Accession DK946406 Tissue t... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946406|Adiantum capillus-veneri...chizosacch... 121 2e-27 sp|O14062|RS12A_SCHPO 40S ribosomal protein S12-A OS=Schizosacch... 121 2e-27 sp|Q54... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946406

  8. AcEST: DK954061 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK954061 - Show DK954061 Clone id TST39A01NGRL0019_H01 Library TST39 Length 594 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_H01. 5' end sequence. Accession DK954061 Tissue t...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954061|Adiantum ...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95406...TST39A01NGRL0019_H01 594 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_H0

  9. AcEST: DK944064 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK944064 CL53Contig1 Show DK944064 Clone id YMU02A01NGRL0004_N23 Library YMU02 Length 49...9 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_N23. 5' end sequence. Accession DK94406...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944064|Adiantum capillus-...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944064|Adiantum capill...YMU02A01NGRL0004_N23 499 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_N2

  10. AcEST: DK954067 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK954067 - Show DK954067 Clone id TST39A01NGRL0019_H07 Library TST39 Length 611 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_H07. 5' end sequence. Accession DK954067 Tissue t...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954067|Adiantum capillus-veneris m... = 5.7 Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 3/36 (8%) Frame = +1 Query: 406...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954067|Adiantum capillus-

  11. AcEST: DK954069 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK954069 CL33Contig1 Show DK954069 Clone id TST39A01NGRL0019_H09 Library TST39 Length 58...8 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_H09. 5' end sequence. Accession DK95406...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954069|Adiantum capillus-vener...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95406...TST39A01NGRL0019_H09 588 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_H0

  12. AcEST: BP912347 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000018_A09 547 Adiantum capillus-veneris mRNA. clone: YMU001_000018_A09. BP91234...7 CL3073Contig1 Show BP912347 Clone id YMU001_000018_A09 Library YMU01 Length 547 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000018_A09. Accession BP912347 Tissue type prothallium Developmental stag... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912347|Adiantum capillus-ven...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91234

  13. AcEST: DK961234 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK961234 CL4158Contig1 Show DK961234 Clone id TST39A01NGRL0009_I23 Library TST39 Length ...599 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_I23. 5' end sequence. Accession DK961234...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961234...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25...TST39A01NGRL0009_I23 599 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_I2

  14. AcEST: DK943673 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK943673 - Show DK943673 Clone id YMU02A01NGRL0003_J08 Library YMU02 Length 561 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J08. 5' end sequence. Accession DK943673 Tissue t...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943673|Adiantum capillus-veneris...database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943673|Adiantum capillus-veneris mRNA, c...YMU02A01NGRL0003_J08 561 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J0

  15. AcEST: DK951367 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK951367 CL3333Contig1 Show DK951367 Clone id TST38A01NGRL0011_D15 Library TST38 Length ...668 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_D15. 5' end sequence. Accession DK951367...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951367...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951367|Adiantum capillus-veneris mRNA...00_PHYPA Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_170943 PE=4 SV=1 Length = 367

  16. AcEST: DK953673 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK953673 CL7Contig10 Show DK953673 Clone id TST39A01NGRL0018_G10 Library TST39 Length 59...7 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G10. 5' end sequence. Accession DK95367...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953673|...ed BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95367...TST39A01NGRL0018_G10 597 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G1

  17. AcEST: DK953670 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK953670 CL238Contig1 Show DK953670 Clone id TST39A01NGRL0018_G07 Library TST39 Length 5...91 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G07. 5' end sequence. Accession DK95367...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953670|A...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953670|Ad...TST39A01NGRL0018_G07 591 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G0

  18. AcEST: DK953674 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK953674 CL1694Contig1 Show DK953674 Clone id TST39A01NGRL0018_G11 Library TST39 Length ...598 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0018_G11. 5' end sequence. Accession DK95367...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953674|Adiantum capillu...50 kDa isoform OS=Neurospora crassa GN=ro-3 PE=3 SV=3 Length = 1367 Score = 42.4 bits (98), Expect = 0.002 I...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953674|Adiantum capillus-veneris mRNA

  19. AcEST: DK943670 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK943670 CL2854Contig1 Show DK943670 Clone id YMU02A01NGRL0003_J05 Library YMU02 Length ...508 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J05. 5' end sequence. Accession DK94367...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943670|Adiantum capillus-vener... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94367...YMU02A01NGRL0003_J05 508 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J0

  20. AcEST: DK943676 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK943676 - Show DK943676 Clone id YMU02A01NGRL0003_J11 Library YMU02 Length 379 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J11. 5' end sequence. Accession DK943676 Tissue t...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943676|Ad...YMU02A01NGRL0003_J11 379 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0003_J1...997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2

  1. AcEST: DK957757 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK957757 CL51Contig1 Show DK957757 Clone id TST39A01NGRL0029_D08 Library TST39 Length 69...2 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D08. 5' end sequence. Accession DK95775...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775...TST39A01NGRL0029_D08 692 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D0

  2. AcEST: DK957753 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK957753 - Show DK957753 Clone id TST39A01NGRL0029_D04 Library TST39 Length 647 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D04. 5' end sequence. Accession DK957753 Tissue t...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775...TST39A01NGRL0029_D04 647 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D0

  3. AcEST: DK947759 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK947759 CL622Contig1 Show DK947759 Clone id YMU02A01NGRL0016_O14 Library YMU02 Length 2...26 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_O14. 5' end sequence. Accession DK94775...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947759|Adiantum cap...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947759|Adiantum capillus...YMU02A01NGRL0016_O14 226 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_O1

  4. AcEST: BP917759 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_B01 501 Adiantum capillus-veneris mRNA. clone: YMU001_000105_B01. BP91775...9 CL1351Contig1 Show BP917759 Clone id YMU001_000105_B01 Library YMU01 Length 501 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000105_B01. Accession BP917759 Tissue type prothallium Developmental stag...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91775...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917759|Adian

  5. AcEST: DK957752 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK957752 CL3195Contig1 Show DK957752 Clone id TST39A01NGRL0029_D03 Library TST39 Length ...641 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D03. 5' end sequence. Accession DK95775... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95775...TST39A01NGRL0029_D03 641 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_D0

  6. AcEST: DK947755 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK947755 CL244Contig1 Show DK947755 Clone id YMU02A01NGRL0016_O09 Library YMU02 Length 2...47 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_O09. 5' end sequence. Accession DK94775..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94775...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947755|Adiantu...YMU02A01NGRL0016_O09 247 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0016_O0

  7. AcEST: DK951980 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK951980 CL72Contig2 Show DK951980 Clone id TST38A01NGRL0012_N18 Library TST38 Length 54...7 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_N18. 5' end sequence. Accession DK951980...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951980..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951980...TST38A01NGRL0012_N18 547 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_N1

  8. AcEST: DK950502 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0008_N06 650 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_N0...6. 5' end sequence. DK950502 CL1Contig3 Show DK950502 Clone id TST38A01NGRL0008_N06 Library TST38 Length 650... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_N06. 5' end sequence. Accession DK950502...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950502|Adiantum... capillus-veneris mRNA, clone: TST38A01NGRL0008_N06, 5' (650 letters) Database: u

  9. AcEST: BP915050 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000065_H08 321 Adiantum capillus-veneris mRNA. clone: YMU001_000065_H08. BP915050 - Show BP915050...is mRNA. clone: YMU001_000065_H08. Accession BP915050 Tissue type prothallium Developmental stage - Contig I...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915050|...2 (56%), Gaps = 3/32 (9%) Frame = -2 Query: 104 AAPLEKRIGEELTLVYVPPH---PLVKHWLSV 18 A P KRI EL V VPPH L +HW+ + Sbjct: 50...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915050

  10. AcEST: DK950504 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0008_N08 642 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_N0...8. 5' end sequence. DK950504 CL3542Contig1 Show DK950504 Clone id TST38A01NGRL0008_N08 Library TST38 Length ...642 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_N08. 5' end sequence. Accession DK95050...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95050...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950504|Adiantum

  11. AcEST: DK960101 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK960101 - Show DK960101 Clone id TST39A01NGRL0006_H22 Library TST39 Length 645 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_H22. 5' end sequence. Accession DK960101 Tissue t... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960101... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960101...20), Expect = 1e-39 Identities = 82/144 (56%), Positives = 101/144 (70%), Gaps = 3/144 (2%) Frame = +1 Query

  12. AcEST: DK951444 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK951444 CL762Contig1 Show DK951444 Clone id TST38A01NGRL0011_G20 Library TST38 Length 6...43 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_G20. 5' end sequence. Accession DK95144...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95144...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951444|Adiantum ...TST38A01NGRL0011_G20 643 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_G2

  13. AcEST: BP914442 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_A01 602 Adiantum capillus-veneris mRNA. clone: YMU001_000059_A01. BP9144...42 CL10Contig1 Show BP914442 Clone id YMU001_000059_A01 Library YMU01 Length 602 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000059_A01. Accession BP914442 Tissue type prothallium Developmental stage ...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144

  14. AcEST: BP921448 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000150_A08 503 Adiantum capillus-veneris mRNA. clone: YMU001_000150_A08. BP92144...8 CL1218Contig1 Show BP921448 Clone id YMU001_000150_A08 Library YMU01 Length 503 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000150_A08. Accession BP921448 Tissue type prothallium Developmental stag...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92144...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921448|Adiantum capillus-veneris m

  15. AcEST: BP921443 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000150_A02 412 Adiantum capillus-veneris mRNA. clone: YMU001_000150_A02. BP92144...3 CL1772Contig1 Show BP921443 Clone id YMU001_000150_A02 Library YMU01 Length 412 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000150_A02. Accession BP921443 Tissue type prothallium Developmental stag...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92144...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921443|Ad

  16. AcEST: BP921446 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000150_A05 493 Adiantum capillus-veneris mRNA. clone: YMU001_000150_A05. BP92144...6 CL3709Contig1 Show BP921446 Clone id YMU001_000150_A05 Library YMU01 Length 493 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000150_A05. Accession BP921446 Tissue type prothallium Developmental stag...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92144...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92144

  17. AcEST: DK956412 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK956412 - Show DK956412 Clone id TST39A01NGRL0025_K19 Library TST39 Length 586 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_K19. 5' end sequence. Accession DK956412 Tissue t...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956412|Adiantum capillus-veneris mRNA,...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956412...TST39A01NGRL0025_K19 586 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_K1

  18. AcEST: DK944326 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK944326 CL14Contig1 Show DK944326 Clone id YMU02A01NGRL0005_L04 Library YMU02 Length 22...6 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L04. 5' end sequence. Accession DK94432...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944326|Adiantum capillus-ve...YMU02A01NGRL0005_L04 226 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L0...997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2

  19. AcEST: DK944325 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK944325 - Show DK944325 Clone id YMU02A01NGRL0005_L03 Library YMU02 Length 545 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L03. 5' end sequence. Accession DK944325 Tissue t...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94432... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94432...YMU02A01NGRL0005_L03 545 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L0

  20. AcEST: BP914327 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F08 581 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F08. BP914327 - Show BP91432...is mRNA. clone: YMU001_000057_F08. Accession BP914327 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914327|Adiantum ... database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914327|Adiantum capillus-veneris mRNA, ... GN=MMAR_5294 PE=4 SV=1 Length = 2432 Score = 37.7 bits (86), Expect = 0.47 Identities = 30/82 (36%), Positi

  1. AcEST: DK954321 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK954321 CL13Contig1 Show DK954321 Clone id TST39A01NGRL0020_C04 Library TST39 Length 62...6 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_C04. 5' end sequence. Accession DK95432...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954321|...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95432...TST39A01NGRL0020_C04 626 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_C0

  2. AcEST: DK954328 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK954328 - Show DK954328 Clone id TST39A01NGRL0020_C11 Library TST39 Length 646 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_C11. 5' end sequence. Accession DK954328 Tissue t...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95432...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95432...TST39A01NGRL0020_C11 646 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_C1

  3. AcEST: BP914322 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F03 608 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F03. BP91432...2 CL46Contig1 Show BP914322 Clone id YMU001_000057_F03 Library YMU01 Length 608 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000057_F03. Accession BP914322 Tissue type prothallium Developmental stage ...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432

  4. AcEST: DK944327 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK944327 - Show DK944327 Clone id YMU02A01NGRL0005_L06 Library YMU02 Length 559 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L06. 5' end sequence. Accession DK944327 Tissue t... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944327|Adiant...database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944327|Adiantum capillus-veneris mRNA, c...YMU02A01NGRL0005_L06 559 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_L0

  5. AcEST: DK957474 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0028_H12 611 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0028_H1...2. 5' end sequence. DK957474 CL43Contig1 Show DK957474 Clone id TST39A01NGRL0028_H12 Library TST39 Length 61...1 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0028_H12. 5' end sequence. Accession DK957474...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957474|Adiantum capillu...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957474|Adiantum capi

  6. AcEST: BP915914 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_D09 270 Adiantum capillus-veneris mRNA. clone: YMU001_000079_D09. BP91591...4 CL728Contig1 Show BP915914 Clone id YMU001_000079_D09 Library YMU01 Length 270 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000079_D09. Accession BP915914 Tissue type prothallium Developmental stage...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915914|Adiantum...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9159

  7. AcEST: BP919210 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000122_E03 243 Adiantum capillus-veneris mRNA. clone: YMU001_000122_E03. BP91921...0 CL1148Contig1 Show BP919210 Clone id YMU001_000122_E03 Library YMU01 Length 243 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000122_E03. Accession BP919210 Tissue type prothallium Developmental stag...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919210|Adiantum capillus-veneris mRNA, c...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91921

  8. AcEST: DK952207 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK952207 - Show DK952207 Clone id TST38A01NGRL0013_H14 Library TST38 Length 584 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_H14. 5' end sequence. Accession DK952207 Tissue t...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95220...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95220...TST38A01NGRL0013_H14 584 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_H1

  9. AcEST: DK961220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK961220 CL403Contig1 Show DK961220 Clone id TST39A01NGRL0009_I09 Library TST39 Length 6...29 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_I09. 5' end sequence. Accession DK961220... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961220...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25...:3389-3402. Query= DK961220|Adiantum capillus-veneris mRNA, clone: TST39A01NGRL00

  10. AcEST: DK962205 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK962205 CL320Contig1 Show DK962205 Clone id TST39A01NGRL0013_D20 Library TST39 Length 6...68 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_D20. 5' end sequence. Accession DK96220...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962205|Ad...TST39A01NGRL0013_D20 668 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_D2...997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2

  11. AcEST: DK962200 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK962200 CL3038Contig1 Show DK962200 Clone id TST39A01NGRL0013_D15 Library TST39 Length ...584 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_D15. 5' end sequence. Accession DK96220...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96220... G + H Sbjct: 113 KPDTFTFPFVLKIAVRVSDVWFGRQIH 139 >sp|Q9SHZ8|PP168_ARATH Pentatricopeptide repeat-containing protein At2g220...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96220

  12. AcEST: DK952201 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK952201 CL1460Contig1 Show DK952201 Clone id TST38A01NGRL0013_H08 Library TST38 Length ...661 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_H08. 5' end sequence. Accession DK95220...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95220... (53%) Frame = +2 Query: 65 AQVPERSSSSLAATVPFLY*QLFHASENQLLEVFFFSALEVKCDAKVTPLME 220 A + E+ +S+L PF + +E ++L...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95220

  13. AcEST: DK951941 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK951941 CL1Contig3 Show DK951941 Clone id TST38A01NGRL0012_M02 Library TST38 Length 593... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_M02. 5' end sequence. Accession DK951941...database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951941|Adiantum capillus-veneris mRNA, c...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951941|Adiantum capillus-...TST38A01NGRL0012_M02 593 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0012_M0

  14. AcEST: BP920047 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_D10 386 Adiantum capillus-veneris mRNA. clone: YMU001_000132_D10. BP92004...7 CL1851Contig1 Show BP920047 Clone id YMU001_000132_D10 Library YMU01 Length 386 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000132_D10. Accession BP920047 Tissue type prothallium Developmental stag... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92004

  15. AcEST: DK962004 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK962004 CL66Contig1 Show DK962004 Clone id TST39A01NGRL0011_L03 Library TST39 Length 68...9 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L03. 5' end sequence. Accession DK962004...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96200...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962004...TST39A01NGRL0011_L03 689 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_L0

  16. AcEST: DK952333 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK952333 - Show DK952333 Clone id TST38A01NGRL0013_M23 Library TST38 Length 588 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_M23. 5' end sequence. Accession DK952333 Tissue t...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952333...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952333...TST38A01NGRL0013_M23 588 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_M2

  17. AcEST: BP917025 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000094_H07 471 Adiantum capillus-veneris mRNA. clone: YMU001_000094_H07. BP917025 - Show BP917025...is mRNA. clone: YMU001_000094_H07. Accession BP917025 Tissue type prothallium Developmental stage - Contig I... programs, Nucleic Acids Res. 25:3389-3402. Query= BP917025|Adiantum capillus-ven...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917025

  18. AcEST: BP917781 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000105_D04 511 Adiantum capillus-veneris mRNA. clone: YMU001_000105_D04. BP917781 - Show BP9177...is mRNA. clone: YMU001_000105_D04. Accession BP917781 Tissue type prothallium Developmental stage - Contig I... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9177... programs, Nucleic Acids Res. 25:3389-3402. Query= BP917781|Adiantum capillus-ven

  19. AcEST: BP921774 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000154_A05 442 Adiantum capillus-veneris mRNA. clone: YMU001_000154_A05. BP921774 - Show BP92177...is mRNA. clone: YMU001_000154_A05. Accession BP921774 Tissue type prothallium Developmental stage - Contig I...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25...: 414 TDILNAQTVSLSNDTIAIKDKADEKIIFLFEASTGKPLGDGKLLSHKNEISEVALDQKGL 473 Query: 177 IADRMLIFIDSNNDLYIACIVK----...bjct: 414 TDILNAQTVSLSNDTIAIKDKADEKIIFLFEASTGKPLGDGKLLSHKNEISEIALDQKGL 473 Query: 177 IADRMLIFIDSNNDLYIACIVK

  20. AcEST: BP920154 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_F09 487 Adiantum capillus-veneris mRNA. clone: YMU001_000133_F09. BP920154 - Show BP92015...is mRNA. clone: YMU001_000133_F09. Accession BP920154 Tissue type prothallium Developmental stage - Contig I...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920154|Adia...abase search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920154|Adiantum

  1. AcEST: DK949406 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK949406 - Show DK949406 Clone id TST38A01NGRL0005_O13 Library TST38 Length 717 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0005_O13. 5' end sequence. Accession DK949406 Tissue t...rch programs, Nucleic Acids Res. 25:3389-3402. Query= DK949406|Adiantum capillus-... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK949406|Adian

  2. AcEST: BP914066 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_E02 515 Adiantum capillus-veneris mRNA. clone: YMU001_000039_E02. BP914066 - Show BP91406...is mRNA. clone: YMU001_000039_E02. Accession BP914066 Tissue type prothallium Developmental stage - Contig I...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91406...h programs, Nucleic Acids Res. 25:3389-3402. Query= BP914066|Adiantum capillus-ve

  3. AcEST: BP921101 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000145_F04 489 Adiantum capillus-veneris mRNA. clone: YMU001_000145_F04. BP921101 - Show BP921101...is mRNA. clone: YMU001_000145_F04. Accession BP921101 Tissue type prothallium Developmental stage - Contig I...se search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921101|Adiantum cap... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921101

  4. AcEST: BP914473 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_C10 217 Adiantum capillus-veneris mRNA. clone: YMU001_000059_C10. BP914473 - Show BP9144...is mRNA. clone: YMU001_000059_C10. Accession BP914473 Tissue type prothallium Developmental stage - Contig I...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9144... search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914473|Adiantum capil

  5. AcEST: DK949343 [AcEST

    Lifescience Database Archive (English)

    Full Text Available man (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids ...AKVIIDPITNRSKGYGFVRFGDENERTRAMSEMNG-VYCSSRPMRISVATTK 645 PS RG KV++DP T SKGYGFV+F DE E+ RA++E G V +P+R+SVA K...YEFFVKVY 120 Query: 478 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMSEMNG-VYCSSRPMRISVATTK...4 KINGKPLPGATPAKRFKLNYATYG---KQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVY 120 Query: 478 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMS...MLYEFFVKVY 120 Query: 478 PSVRGAKVIIDPITNRSKGYGFVRFGDENERTRAMSEMNG-VYCSSRPMRISVATTK 645 PS RG KV++D T SKGYGF

  6. AcEST: DK961517 [AcEST

    Lifescience Database Archive (English)

    Full Text Available protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961517|Adiantum capillus-veneri...Query: 24 RDIAKKKRKGADQLPETVKVDCKKPQEEAHHGFATVEESTEKCEADDSEQKAALQERAMS 203 R+ A+K...E+ + E Sbjct: 14 RVPPEFEPDMEKIKRRLLKYGVDPTPKILNNLRKK--EIQKHNRRTKRETESEAEVYTEA 71 Query: 171 QKAALQERAMSAEMEA...+3 Query: 24 RDIAKKKRKGADQLPETVKVDCKKPQEEAHHGFATVEESTEKCEADDSEQKAALQERAMS 203 R+ A+K+++ + E +K + E+ AT +E K ...KKPQEEAHHGFATVEESTEKCEADDSEQKAALQERAMS 203 R+ A+K+++ + E +K + E+ AT +E K E + + K

  7. AcEST: DK960524 [AcEST

    Lifescience Database Archive (English)

    Full Text Available of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960524|Adiantum capillus-vene...EMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLEL 241 Query: 212 ARNPELMREMMRNTDRAMSNIEASPEGFNMLRRM...PEISHMLNNPDIMRQTLEL 232 Query: 212 ARNPELMREMMRNTDRAMSNIEASPEGFNMLRRMYETVEEP 334 ARNP +M+EMMRN DRA+SN+E+ P G...E+ H+LN+P +RQTL+ Sbjct: 182 NPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLEL 241 Query: 212 ARNPELMREMMRNTDRAMS...PLVQDMMSNPDLMRHMIMANPQMQQLMERNPEISHMLNNPELMRQTMEL 246 Query: 212 ARNPELMREMMRNTDRAMSNIEASPEGFNMLRRMYETVEEP 3

  8. AcEST: BP919212 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000122_E05 508 Adiantum capillus-veneris mRNA. clone: YMU001_000122_E05. BP919212 - Show BP91921...is mRNA. clone: YMU001_000122_E05. Accession BP919212 Tissue type prothallium Developmental stage - Contig I... programs, Nucleic Acids Res. 25:3389-3402. Query= BP919212|Adiantum capillus-ven...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919212|Adiantum capillus-

  9. Nuclear power economic database

    International Nuclear Information System (INIS)

    Nuclear power economic database (NPEDB), based on ORACLE V6.0, consists of three parts, i.e., economic data base of nuclear power station, economic data base of nuclear fuel cycle and economic database of nuclear power planning and nuclear environment. Economic database of nuclear power station includes data of general economics, technique, capital cost and benefit, etc. Economic database of nuclear fuel cycle includes data of technique and nuclear fuel price. Economic database of nuclear power planning and nuclear environment includes data of energy history, forecast, energy balance, electric power and energy facilities

  10. An Interoperable Cartographic Database

    Directory of Open Access Journals (Sweden)

    Slobodanka Ključanin

    2007-05-01

    Full Text Available The concept of producing a prototype of interoperable cartographic database is explored in this paper, including the possibilities of integration of different geospatial data into the database management system and their visualization on the Internet. The implementation includes vectorization of the concept of a single map page, creation of the cartographic database in an object-relation database, spatial analysis, definition and visualization of the database content in the form of a map on the Internet. 

  11. School version of ESTE EU

    International Nuclear Information System (INIS)

    ESTE EU is information system and software for radiological impacts assessment to the territory of the country in case of radiation accident inside/outside of the country .The program enables to model dispersion of radioactive clouds in small-scale and meso-scale. The system enables the user to estimate prediction of the source term (release to the atmosphere ) for any point of radiation/nuclear accident in Europe (for any point of the release, but especially for the sites of European power reactors ). The system enables to utilize results of real radiological monitoring in the process of source term estimation. Radiological impacts of release to the atmosphere are modelled and calculated across the Europe and displayed in the geographical information system (GIS). The school version of ESTE EU is intended for students of the universities which are interested in or could work in the field of emergency response, radiological and nuclear accidents, dispersion modelling, radiological impacts calculation and urgent or preventive protective measures implementation. The school version of ESTE EU is planned to be donated to specialized departments of faculties in Slovakia, Czech Republic, etc. System can be fully operated in Slovak, Czech or English language. (authors)

  12. Database design and database administration for a kindergarten

    OpenAIRE

    Vítek, Daniel

    2009-01-01

    The bachelor thesis deals with creation of database design for a standard kindergarten, installation of the designed database into the database system Oracle Database 10g Express Edition and demonstration of the administration tasks in this database system. The verification of the database was proved by a developed access application.

  13. AcEST: BP912003 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000011_H07 455 Adiantum capillus-veneris mRNA. clone: YMU001_000011_H07. BP9120...03 CL3942Contig1 Show BP912003 Clone id YMU001_000011_H07 Library YMU01 Length 455 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000011_H07. Accession BP912003 Tissue type prothallium Developmental stag..., Nucleic Acids Res. 25:3389-3402. Query= BP912003|Adiantum capillus-veneris mRNA...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  14. AcEST: BP912057 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_E09 528 Adiantum capillus-veneris mRNA. clone: YMU001_000012_E09. BP9120...57 CL1892Contig1 Show BP912057 Clone id YMU001_000012_E09 Library YMU01 Length 528 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_E09. Accession BP912057 Tissue type prothallium Developmental stag...Acids Res. 25:3389-3402. Query= BP912057|Adiantum capillus-veneris mRNA, clone: YMU001_000012_E09. (528 lett... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  15. AcEST: BP921209 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000147_A04 535 Adiantum capillus-veneris mRNA. clone: YMU001_000147_A04. BP921209 - Show BP92120...is mRNA. clone: YMU001_000147_A04. Accession BP921209 Tissue type prothallium Developmental stage - Contig I...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92120...grams, Nucleic Acids Res. 25:3389-3402. Query= BP921209|Adiantum capillus-veneris mRNA, clone: YMU001_000147

  16. AcEST: DK961202 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK961202 CL188Contig1 Show DK961202 Clone id TST39A01NGRL0009_H14 Library TST39 Length 6...38 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_H14. 5' end sequence. Accession DK96120... Acids Res. 25:3389-3402. Query= DK961202|Adiantum capillus-veneris mRNA, clone: ...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96120...WGFFQV+NHG+D ++ +++ +F LP+ E+ Sbjct: 61 LLKGKTDELQRELSKLAASCEEWGFFQVINHGIDLGLLESIEKAAMDFFMLPLEEKQKYA 120 Que

  17. AcEST: BP912036 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_C09 555 Adiantum capillus-veneris mRNA. clone: YMU001_000012_C09. BP912036 - Show BP9120...is mRNA. clone: YMU001_000012_C09. Accession BP912036 Tissue type prothallium Developmental stage - Contig I...ms, Nucleic Acids Res. 25:3389-3402. Query= BP912036|Adiantum capillus-veneris mRNA, clone: YMU001_000012_C0... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912036|Adiantum capillus-ven

  18. AcEST: BP912042 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_D05 541 Adiantum capillus-veneris mRNA. clone: YMU001_000012_D05. BP912042 - Show BP9120...is mRNA. clone: YMU001_000012_D05. Accession BP912042 Tissue type prothallium Developmental stage - Contig I...c Acids Res. 25:3389-3402. Query= BP912042|Adiantum capillus-veneris mRNA, clone: YMU001_000012_D05. (541 le...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912042|Adiantum capil

  19. AcEST: BP912056 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_E08 538 Adiantum capillus-veneris mRNA. clone: YMU001_000012_E08. BP912056 - Show BP9120...is mRNA. clone: YMU001_000012_E08. Accession BP912056 Tissue type prothallium Developmental stage - Contig I...base search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912056|Adiantum capillus-veneris mRNA, clone... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  20. AcEST: BP921120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000146_A02 427 Adiantum capillus-veneris mRNA. clone: YMU001_000146_A02. BP921120 - Show BP921120...is mRNA. clone: YMU001_000146_A02. Accession BP921120 Tissue type prothallium Developmental stage - Contig I... Acids Res. 25:3389-3402. Query= BP921120|Adiantum capillus-veneris mRNA, clone: ...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921120|Adian