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Sample records for artichoke est database

  1. CyMSatDB: The Globe Artichoke (Cynara cardunculus var. scolymus) Microsatellite Database

    DEFF Research Database (Denmark)

    Portis, Ezio; Portis, Flavio; Valente, Luisa;

    The first high-quality genome assembly of the globe artichoke (Cynara cardunculus var. scolymus) has been recently produced at the DISAFA (Plant Genetics and Breeding) within the Compositae Genome Project (Scaglione et al. 2014, XXII PAG Conference). The assembly contains 13K scaffolds (N50= 125...... in a MySQL database and provides an effective and responsive interface developed in PHP. To cater the customized needs of wet lab, features with a novelty of an automated primer designing tool is added. The feature of user defined primer designing has great advantage in terms of precise selection from...

  2. CyMSatDB: The Globe Artichoke (Cynara cardunculus var. scolymus) Microsatellite Database

    DEFF Research Database (Denmark)

    Portis, Ezio; Portis, Flavio; Valente, Luisa;

    2015-01-01

    The first high-quality genome assembly of the globe artichoke (Cynara cardunculus var. scolymus) has been recently produced at the DISAFA (Plant Genetics and Breeding) within the Compositae Genome Project (Scaglione et al. 2014, XXII PAG Conference). The assembly contains 13K scaffolds (N50= 125...... in a MySQL database and provides an effective and responsive interface developed in PHP. To cater the customized needs of wet lab, features with a novelty of an automated primer designing tool is added. The feature of user defined primer designing has great advantage in terms of precise selection from...

  3. An EST database from saffron stigmas

    Directory of Open Access Journals (Sweden)

    Chiusano Maria Luisa

    2007-10-01

    Full Text Available Abstract Background Saffron (Crocus sativus L., Iridaceae flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part – the stigmas – are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. Results We produced 6,603 high quality Expressed Sequence Tags (ESTs from a saffron stigma cDNA library. This collection is accessible and searchable through the Saffron Genes database http://www.saffrongenes.org. The ESTs have been grouped into 1,893 Clusters, each corresponding to a different expressed gene, and annotated. The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database, allowing users to investigate sequence qualities and EST structural features (vector contamination, repeat regions. The saffron stigma transcriptome contains a series of interesting sequences (putative sex determination genes, lipid and carotenoid metabolism enzymes, transcription factors. Conclusion The Saffron Genes database represents the first reference collection for the genomics of Iridaceae, for the molecular biology of stigma biogenesis, as well as for the metabolic pathways underlying saffron secondary metabolism.

  4. Signaling pathways in a Citrus EST database

    Directory of Open Access Journals (Sweden)

    Angela Mehta

    2007-01-01

    Full Text Available Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis was launched in order to sequence Citrus ESTs (expressed sequence tags from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile. Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

  5. Update History of This Database - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ClEST Up...date History of This Database Date Update contents 2012/05/22 ClEST English archive site is opened. ...Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Da...tabase Site Policy | Contact Us Update History of This Database - ClEST | LSDB Archive ...

  6. Update History of This Database - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AcEST Up...date History of This Database Date Update contents 2013/01/10 Errors found on AcEST Conting data hav...iew/acest/ ) is released. Joomla SEF URLs by Artio About This Database Database Description Download License Up...date History of This Database Site Policy | Contact Us Update History of This Database - AcEST | LSDB Archive ...

  7. A Genome-Wide Survey of the Microsatellite Content of the Globe Artichoke Genome and the Development of a Web-Based Database.

    Science.gov (United States)

    Portis, Ezio; Portis, Flavio; Valente, Luisa; Moglia, Andrea; Barchi, Lorenzo; Lanteri, Sergio; Acquadro, Alberto

    2016-01-01

    The recently acquired genome sequence of globe artichoke (Cynara cardunculus var. scolymus) has been used to catalog the genome's content of simple sequence repeat (SSR) markers. More than 177,000 perfect SSRs were revealed, equivalent to an overall density across the genome of 244.5 SSRs/Mbp, but some 224,000 imperfect SSRs were also identified. About 21% of these SSRs were complex (two stretches of repeats separated by density across the gene space of 32,5 and 44,9 SSRs/Mbp for perfect and imperfect motifs, respectively. A putative function has been assigned, using the gene ontology approach, to the set of genes harboring at least one SSR. The same search parameters were applied to reveal the SSR content of 14 other plant species for which genome sequence is available. Certain species-specific SSR motifs were identified, along with a hexa-nucleotide motif shared only with the other two Compositae species (sunflower (Helianthus annuus) and horseweed (Conyza canadensis)) included in the study. Finally, a database, called "Cynara cardunculus MicroSatellite DataBase" (CyMSatDB) was developed to provide a searchable interface to the SSR data. CyMSatDB facilitates the retrieval of SSR markers, as well as suggested forward and reverse primers, on the basis of genomic location, genomic vs genic context, perfect vs imperfect repeat, motif type, motif sequence and repeat number. The SSR markers were validated via an in silico based PCR analysis adopting two available assembled transcriptomes, derived from contrasting globe artichoke accessions, as templates.

  8. AcEST Contig - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...contig.zip File URL: ftp://ftp.biosciencedbc.jp/archive/acest/LATEST/acestcontig....zip File size: 1.34MB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acestcontig#en Da...Joomla SEF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us AcEST Contig - AcEST | LSDB Archive ...

  9. A Genome-Wide Survey of the Microsatellite Content of the Globe Artichoke Genome and the Development of a Web-Based Database

    Science.gov (United States)

    Portis, Ezio; Portis, Flavio; Valente, Luisa; Moglia, Andrea; Barchi, Lorenzo; Lanteri, Sergio; Acquadro, Alberto

    2016-01-01

    The recently acquired genome sequence of globe artichoke (Cynara cardunculus var. scolymus) has been used to catalog the genome’s content of simple sequence repeat (SSR) markers. More than 177,000 perfect SSRs were revealed, equivalent to an overall density across the genome of 244.5 SSRs/Mbp, but some 224,000 imperfect SSRs were also identified. About 21% of these SSRs were complex (two stretches of repeats separated by <100 nt). Some 73% of the SSRs were composed of dinucleotide motifs. The SSRs were categorized for the numbers of repeats present, their overall length and were allocated to their linkage group. A total of 4,761 perfect and 6,583 imperfect SSRs were present in 3,781 genes (14.11% of the total), corresponding to an overall density across the gene space of 32,5 and 44,9 SSRs/Mbp for perfect and imperfect motifs, respectively. A putative function has been assigned, using the gene ontology approach, to the set of genes harboring at least one SSR. The same search parameters were applied to reveal the SSR content of 14 other plant species for which genome sequence is available. Certain species-specific SSR motifs were identified, along with a hexa-nucleotide motif shared only with the other two Compositae species (sunflower (Helianthus annuus) and horseweed (Conyza canadensis)) included in the study. Finally, a database, called “Cynara cardunculus MicroSatellite DataBase” (CyMSatDB) was developed to provide a searchable interface to the SSR data. CyMSatDB facilitates the retrieval of SSR markers, as well as suggested forward and reverse primers, on the basis of genomic location, genomic vs genic context, perfect vs imperfect repeat, motif type, motif sequence and repeat number. The SSR markers were validated via an in silico based PCR analysis adopting two available assembled transcriptomes, derived from contrasting globe artichoke accessions, as templates. PMID:27648830

  10. SPODOBASE : an EST database for the lepidopteran crop pest Spodoptera

    Directory of Open Access Journals (Sweden)

    Sabourault Cécile

    2006-06-01

    Full Text Available Abstract Background The Lepidoptera Spodoptera frugiperda is a pest which causes widespread economic damage on a variety of crop plants. It is also well known through its famous Sf9 cell line which is used for numerous heterologous protein productions. Species of the Spodoptera genus are used as model for pesticide resistance and to study virus host interactions. A genomic approach is now a critical step for further new developments in biology and pathology of these insects, and the results of ESTs sequencing efforts need to be structured into databases providing an integrated set of tools and informations. Description The ESTs from five independent cDNA libraries, prepared from three different S. frugiperda tissues (hemocytes, midgut and fat body and from the Sf9 cell line, are deposited in the database. These tissues were chosen because of their importance in biological processes such as immune response, development and plant/insect interaction. So far, the SPODOBASE contains 29,325 ESTs, which are cleaned and clustered into non-redundant sets (2294 clusters and 6103 singletons. The SPODOBASE is constructed in such a way that other ESTs from S. frugiperda or other species may be added. User can retrieve information using text searches, pre-formatted queries, query assistant or blast searches. Annotation is provided against NCBI, UNIPROT or Bombyx mori ESTs databases, and with GO-Slim vocabulary. Conclusion The SPODOBASE database provides integrated access to expressed sequence tags (EST from the lepidopteran insect Spodoptera frugiperda. It is a publicly available structured database with insect pest sequences which will allow identification of a number of genes and comprehensive cloning of gene families of interest for scientific community. SPODOBASE is available from URL: http://bioweb.ensam.inra.fr/spodobase

  11. WildSilkbase: An EST database of wild silkmoths

    Directory of Open Access Journals (Sweden)

    Nagaraju J

    2008-07-01

    Full Text Available Abstract Background Functional genomics has particular promise in silkworm biology for identifying genes involved in a variety of biological functions that include: synthesis and secretion of silk, sex determination pathways, insect-pathogen interactions, chorionogenesis, molecular clocks. Wild silkmoths have hardly been the subject of detailed scientific investigations, owing largely to non-availability of molecular and genetic data on these species. As a first step, in the present study we generated large scale expressed sequence tags (EST in three economically important species of wild silkmoths. In order to make these resources available for the use of global scientific community, an EST database called 'WildSilkbase' was developed. Description WildSilkbase is a catalogue of ESTs generated from several tissues at different developmental stages of 3 economically important saturniid silkmoths, an Indian golden silkmoth, Antheraea assama, an Indian tropical tasar silkmoth, A. mylitta and eri silkmoth, Samia cynthia ricini. Currently the database is provided with 57,113 ESTs which are clustered and assembled into 4,019 contigs and 10,019 singletons. Data can be browsed and downloaded using a standard web browser. Users can search the database either by BLAST query, keywords or Gene Ontology query. There are options to carry out searches for species, tissue and developmental stage specific ESTs in BLAST page. Other features of the WildSilkbase include cSNP discovery, GO viewer, homologue finder, SSR finder and links to all other related databases. The WildSilkbase is freely available from http://www.cdfd.org.in/wildsilkbase/. Conclusion A total of 14,038 putative unigenes was identified in 3 species of wild silkmoths. These genes provide important resources to gain insight into the functional and evolutionary study of wild silkmoths. We believe that WildSilkbase will be extremely useful for all those researchers working in the areas of

  12. MELOGEN: an EST database for melon functional genomics

    Directory of Open Access Journals (Sweden)

    Puigdomènech Pere

    2007-09-01

    Full Text Available Abstract Background Melon (Cucumis melo L. is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs from eight normalized cDNA libraries from different tissues in different physiological conditions. Results We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs and 10,614 unclustered sequences (singletons. Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs and 356 single nucleotide polymorphisms (SNPs were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. Conclusion The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of

  13. Fructans of Jerusalem artichokes

    DEFF Research Database (Denmark)

    Rumessen, J J; Bodé, S; Hamberg, O;

    1990-01-01

    Fructans are naturally occurring plant oligosaccharides with sweetening properties. Fructans (FAs) isolated from Jerusalem artichokes (Helianthus tuberosus) were studied with respect to intestinal handling and influence on blood glucose (BG), insulin, and C-peptide responses in eight healthy...

  14. Genes associated with hypersensitive response (HR in the citrus EST database (CitEST

    Directory of Open Access Journals (Sweden)

    Simone Guidetti-Gonzalez

    2007-01-01

    Full Text Available Plants are continuously exposed to pathogen attack, but successful infection is rare because they protect themselves against pathogens using a wide range of response mechanisms. One of them is the hypersensitive response (HR, which is a form of cell death often associated with plant resistance to pathogen infection to prevent the spreadsebpg@cnpq.br sebpg@cnpq.br of the potential pathogen from infected to uninfected tissues. Cell death is activated by recognition of pathogen-derived molecules by the resistance (R gene products, and is associated with the massive accumulation of reactive oxygen species (ROS, salicylic acid (SA, and other pro-death signals such as nitric oxide (NO. The analysis of the citrus EST (CitEST database revealed the presence of putative genes likely to be involved in HR through their products, like metacaspases, lipoxygenases, phospholipases, pathogenesis-related proteins, glutathione transferases/peroxidases, enzymes involved in the phenylpropanoid pathway and in the formation and detoxification of ROS, as well as those involved in the formation and regulation of ion channels, SA and NO. By analysis of the EST database of Citrus, it was possible to identify several putative genes that code for key enzymes involved in HR triggering and also in plant defense against biotic and abiotic stress.

  15. Download - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...t This Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - AcEST | LSDB Archive ...

  16. Download - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...bout This Database Database Description Download License Update History of This Database Site Policy | Contact Us Download - ClEST | LSDB Archive ...

  17. GoSh: a goat and sheep ESTs database

    Directory of Open Access Journals (Sweden)

    P. Mariani

    2010-04-01

    Full Text Available The GoSh database (http://www.itb.cnr.it/gosh/ is an online resource including expressedsequence tags (ESTs from Ovis aries and Capra hircus. A total of 58,990 sheep and goat sequences were downloadedfrom GenBank and processed by a semi-automated pipeline, integrating public programs and Perl scripts.Data were collected in a MySQL database, which can be queried via a PHP-based web interface. Sequences wereassembled and a unigene dataset was defined. Three annotation procedures were carried out on all the ESTsequences and all the contig consensus sequences. A procedure was also implemented to infer statistical classificationamong Gene Ontology (GO categories from the ontology occurrences related to the sequences included in thedatabase. A number of programs were used to extract features and give significance to rough sequences. Amongthese, AutoSNP was used to perform putative SNP detection. Further analyses were performed on the GoSh dbdataset, including tandem repeats search and protein patterns identification. The web interface allows users toretrieve significant data and correspondent external links and to download selected sequences and accessory informationin different formats. The resulting web site is a resource of data and links related to goat and sheepexpressed genes.

  18. Putative resistance genes in the CitEST database

    Directory of Open Access Journals (Sweden)

    Simone Guidetti-Gonzalez

    2007-01-01

    Full Text Available Disease resistance in plants is usually associated with the activation of a wide variety of defense responses to prevent pathogen replication and/or movement. The ability of the host plant to recognize the pathogen and to activate defense responses is regulated by direct or indirect interaction between the products of plant resistance (R and pathogen avirulence (Avr genes. Attempted infection of plants by avirulent pathogens elicits a battery of defenses often followed by the collapse of the challenged host cells. Localized host cell death may help to prevent the pathogen from spreading to uninfected tissues, known as hypersensitive response (HR. When either the plant or the pathogen lacks its cognate gene, activation of the plant’s defense responses fails to occur or is delayed and does not prevent pathogen colonization. In the CitEST database, we identified 1,300 reads related to R genes in Citrus which have been reported in other plant species. These reads were translated in silico, and alignments of their amino acid sequences revealed the presence of characteristic domains and motifs that are specific to R gene classes. The description of the reads identified suggests that they function as resistance genes in citrus.

  19. Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum transcriptome

    Directory of Open Access Journals (Sweden)

    Kim Woo Taek

    2008-10-01

    Full Text Available Abstract Background There is no dedicated database available for Expressed Sequence Tags (EST of the chili pepper (Capsicum annuum, although the interest in a chili pepper EST database is increasing internationally due to the nutritional, economic, and pharmaceutical value of the plant. Recent advances in high-throughput sequencing of the ESTs of chili pepper cv. Bukang have produced hundreds of thousands of complementary DNA (cDNA sequences. Therefore, a chili pepper EST database was designed and constructed to enable comprehensive analysis of chili pepper gene expression in response to biotic and abiotic stresses. Results We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO, and MIPS Functional Catalogue (FunCat. The Pepper EST database is designed to provide a workbench for (i identifying unigenes in pepper plants, (ii analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/. Conclusion The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.

  20. License - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ClEST License Lice... with the terms and conditions of the license described below. The license specifies the license terms regar...ding the use of this database and the requirements you must follow in using this database. The lice...an . If you use data from this database, please be sure attribute this database as follows: ClEST © Minoru Moriyama (AIST) lice...of the Creative Commons Attribution-Share Alike 2.1 Japan is found here . With regard to this database, you are lice

  1. REDVET está indexada en Academia Journals Database

    Directory of Open Access Journals (Sweden)

    Redaccion Veterinaria.org

    2007-12-01

    Full Text Available El slogan de la Academia Journals Database, base de datos y directorio bibliográfico es “La difusión de conocimientos científicos de calidad controlada” por lo que es una satisfacción para REDVET que se nos valore por esta institución como una revista de calidad.

  2. All 5' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...e name: CSV: kome_est_5end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_5end_al...p://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_5end_all.fasta.zip File size: 27 MB Simple search URL ...License Update History of This Database Site Policy | Contact Us All 5' EST - KOME | LSDB Archive ...

  3. All 3' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...e name: CSV: kome_est_3end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_3end_al...p://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_3end_all.fasta.zip File size: 76 MB Simple search URL ...License Update History of This Database Site Policy | Contact Us All 3' EST - KOME | LSDB Archive ...

  4. Database Description - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available d - Referenced database - Entry list - Query search Not available Web services No...t available URL of Web services - Need for user registration - Joomla SEF URLs by Artio About This Database

  5. EST Table - KAIKOcDNA | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ber 2011. Data file File name: kaiko_cdna_main.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...atabase Database Description Download License Update History of This Database Site Policy | Contact Us EST Table - KAIKOcDNA | LSDB Archive ...

  6. Clone - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...ularius EST library Data file File name: clest_clone.zip File URL: ftp://ftp.biosciencedbc.jp/archive/clest/...iption Download License Update History of This Database Site Policy | Contact Us Clone - ClEST | LSDB Archive ...

  7. SolEST database: a "one-stop shop" approach to the study of Solanaceae transcriptomes

    Directory of Open Access Journals (Sweden)

    Chiusano Maria

    2009-11-01

    Full Text Available Abstract Background Since no genome sequences of solanaceous plants have yet been completed, expressed sequence tag (EST collections represent a reliable tool for broad sampling of Solanaceae transcriptomes, an attractive route for understanding Solanaceae genome functionality and a powerful reference for the structural annotation of emerging Solanaceae genome sequences. Description We describe the SolEST database http://biosrv.cab.unina.it/solestdb which integrates different EST datasets from both cultivated and wild Solanaceae species and from two species of the genus Coffea. Background as well as processed data contained in the database, extensively linked to external related resources, represent an invaluable source of information for these plant families. Two novel features differentiate SolEST from other resources: i the option of accessing and then visualizing Solanaceae EST/TC alignments along the emerging tomato and potato genome sequences; ii the opportunity to compare different Solanaceae assemblies generated by diverse research groups in the attempt to address a common complaint in the SOL community. Conclusion Different databases have been established worldwide for collecting Solanaceae ESTs and are related in concept, content and utility to the one presented herein. However, the SolEST database has several distinguishing features that make it appealing for the research community and facilitates a "one-stop shop" for the study of Solanaceae transcriptomes.

  8. Database Description - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available title: Author name(s): Journal: Pubmed ID: Original website information Database ... funding Research funded by the basic research budget of Tokyo Metropolitan University Reference(s) Article

  9. Cluster - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...including Blastx search results and GO annotations Data file File name: clest_cluster.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...istory of This Database Site Policy | Contact Us Cluster - ClEST | LSDB Archive ...

  10. SSRs and INDELs mined from the sunflower EST database: abundance, polymorphisms, and cross-taxa utility.

    Science.gov (United States)

    Heesacker, Adam; Kishore, Venkata K; Gao, Wenxiang; Tang, Shunxue; Kolkman, Judith M; Gingle, Alan; Matvienko, Marta; Kozik, Alexander; Michelmore, Richard M; Lai, Zhao; Rieseberg, Loren H; Knapp, Steven J

    2008-11-01

    Simple sequence repeats (SSRs) are abundant and frequently highly polymorphic in transcribed sequences and widely targeted for marker development in eukaryotes. Sunflower (Helianthus annuus) transcript assemblies were built and mined to identify SSRs and insertions-deletions (INDELs) for marker development, comparative mapping, and other genomics applications in sunflower. We describe the spectrum and frequency of SSRs identified in the sunflower EST database, a catalog of 16,643 EST-SSRs, a collection of 484 EST-SSR and 43 EST-INDEL markers developed from common sunflower ESTs, polymorphisms of the markers among the parents of several intraspecific and interspecific mapping populations, and the transferability of the markers to closely and distantly related species in the Compositae. Of 17,904 unigenes in the transcript assembly, 1,956 (10.9%) harbored one or more SSRs with repeat counts of n > or = 5. EST-SSR markers were 1.6-fold more polymorphic among exotic than elite genotypes and 0.7-fold less polymorphic than non-genic SSR markers. Of 466 EST-SSR or INDEL markers screened for cross-species amplification and polymorphisms, 413 (88.6%) amplified alleles from one or more wild species (H. argophyllus, H. tuberosus, H. anomalus, H. paradoxus, and H. deserticola), whereas 69 (14.8%) amplified alleles from safflower (Carthamus tinctorius) and 67 (14.4%) amplified alleles from lettuce (Lactuca sativa); hence, only a fraction were transferable to distantly related genera in the Compositae, whereas most were transferable to wild relatives of H. annuus. Several thousand additional SSRs were identified in the EST database and supply a wealth of templates for EST-SSR marker development in sunflower.

  11. License - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us AcEST Lice...e terms and conditions of the Standard License and the Additional License described below. The Standard License specifies the lice...ements you must follow in using this database. The Additional License specifies those items that are excepti...onally permitted even though they are generally prohibited in the Standard License. Standard License The Standard Lice...nse for this database is the license specified in the Creative Commons

  12. Analysis of newly established EST databases reveals similarities between heart regeneration in newt and fish

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    Weis Patrick

    2010-01-01

    Full Text Available Abstract Background The newt Notophthalmus viridescens possesses the remarkable ability to respond to cardiac damage by formation of new myocardial tissue. Surprisingly little is known about changes in gene activities that occur during the course of regeneration. To begin to decipher the molecular processes, that underlie restoration of functional cardiac tissue, we generated an EST database from regenerating newt hearts and compared the transcriptional profile of selected candidates with genes deregulated during zebrafish heart regeneration. Results A cDNA library of 100,000 cDNA clones was generated from newt hearts 14 days after ventricular injury. Sequencing of 11520 cDNA clones resulted in 2894 assembled contigs. BLAST searches revealed 1695 sequences with potential homology to sequences from the NCBI database. BLAST searches to TrEMBL and Swiss-Prot databases assigned 1116 proteins to Gene Ontology terms. We also identified a relatively large set of 174 ORFs, which are likely to be unique for urodele amphibians. Expression analysis of newt-zebrafish homologues confirmed the deregulation of selected genes during heart regeneration. Sequences, BLAST results and GO annotations were visualized in a relational web based database followed by grouping of identified proteins into clusters of GO Terms. Comparison of data from regenerating zebrafish hearts identified biological processes, which were uniformly overrepresented during cardiac regeneration in newt and zebrafish. Conclusion We concluded that heart regeneration in newts and zebrafish led to the activation of similar sets of genes, which suggests that heart regeneration in both species might follow similar principles. The design of the newly established newt EST database allows identification of molecular pathways important for heart regeneration.

  13. Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database

    Institute of Scientific and Technical Information of China (English)

    Bao-Long NIU; Zhi-Qi MENG; Yue-Zhi TAO; Shun-Lin LU; Hong-Biao WENG; Li-Hua HE; Wei-Feng SHEN

    2005-01-01

    We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins.

  14. Mining, characterization and validation of EST derived microsatellites from the transcriptome database of Allium sativum L

    Science.gov (United States)

    Chand, Subodh Kumar; Nanda, Satyabrata; Rout, Ellojita; Joshi, Raj Kumar

    2015-01-01

    Expressed Sequence Tags (ESTs) with comprehensive transcript information are valuable resources for development of molecular markers as they are derived from conserved genic regions. The present study highlights the mining of EST database to deduce the class I hyper variable SSRs in A. sativum. From 21694 garlic EST sequences, 642 non-redundant SSRs were identified with an average frequency of 1 per 14.9 kb of garlic transcriptome. The most abundant SSR motifs were the mononucleotides (32.86%) followed by trinucleotides (28.50%) and dinucleotides (13.39%). Among the individual SSRs, (A/T)n accounted for the highest number (137; 21.33%) followed by (G/C)n (74; 11.52%) and (AAG)n (63;9.81%). Primers designed from a robust set of 7 AsESTSSRs resulted in the amplification of 63 polymorphic alleles in 14 accessions of garlic. The resolving power of the markers varied from 4.286 (AsSSR7) to 18.143 (AsSSR13) while the average marker index (MI) was 5.087. These EST-SSRs markers for garlic could be useful for the improvement of garlic linkage map and could be used for evaluating genetic variation and comparative genomics studies in Allium species. PMID:25987765

  15. Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining

    Institute of Scientific and Technical Information of China (English)

    ZHAN Aibin; BAO Zhenmin; WANG Mingling; CHANG Dan; YUAN Jian; WANG Xiaolong; HU Xiaoli; LIANG Chengzhu; HU Jingjie

    2008-01-01

    The EST database of the Pacific abalone (Haliotis discus) was mined for developing mierosatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more mierosatellites. Based on the length and GC content of the flanking regions, duster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify seorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Hoand He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.

  16. AcEST(EST sequences of Adiantum capillus-veneris and their annotation) - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available AcEST AcEST(EST sequences of Adiantum capillus-veneris and their annotation) Data detail Data name AcEST(EST... sequences of Adiantum capillus-veneris and their annotation) Description of data contents EST sequence of Adiantum capill...db/view/archive_acest#en Data acquisition method Capillary sequencer Data analysi...atabases) Number of data entries Adiantum capillus-veneris ESTs: 30,540. Data item Description Clone id Clon...e ID of EST sequence of Adiantum capillus-veneris. Library Library name Length Nu

  17. Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP estmap2001 Statistics... information of rice EST mapping results Data detail Data name Statistics informati...of This Database Site Policy | Contact Us Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive ...

  18. An elm EST database for identifying leaf beetle egg-induced defense genes

    Directory of Open Access Journals (Sweden)

    Büchel Kerstin

    2012-06-01

    Full Text Available Abstract Background Plants can defend themselves against herbivorous insects prior to the onset of larval feeding by responding to the eggs laid on their leaves. In the European field elm (Ulmus minor, egg laying by the elm leaf beetle ( Xanthogaleruca luteola activates the emission of volatiles that attract specialised egg parasitoids, which in turn kill the eggs. Little is known about the transcriptional changes that insect eggs trigger in plants and how such indirect defense mechanisms are orchestrated in the context of other biological processes. Results Here we present the first large scale study of egg-induced changes in the transcriptional profile of a tree. Five cDNA libraries were generated from leaves of (i untreated control elms, and elms treated with (ii egg laying and feeding by elm leaf beetles, (iii feeding, (iv artificial transfer of egg clutches, and (v methyl jasmonate. A total of 361,196 ESTs expressed sequence tags (ESTs were identified which clustered into 52,823 unique transcripts (Unitrans and were stored in a database with a public web interface. Among the analyzed Unitrans, 73% could be annotated by homology to known genes in the UniProt (Plant database, particularly to those from Vitis, Ricinus, Populus and Arabidopsis. Comparative in silico analysis among the different treatments revealed differences in Gene Ontology term abundances. Defense- and stress-related gene transcripts were present in high abundance in leaves after herbivore egg laying, but transcripts involved in photosynthesis showed decreased abundance. Many pathogen-related genes and genes involved in phytohormone signaling were expressed, indicative of jasmonic acid biosynthesis and activation of jasmonic acid responsive genes. Cross-comparisons between different libraries based on expression profiles allowed the identification of genes with a potential relevance in egg-induced defenses, as well as other biological processes, including signal transduction

  19. Use of EST database markers from M. truncatula in the transferability to other forage legumes.

    Science.gov (United States)

    Chandra, Amaresh

    2011-05-01

    In general tropical forage legumes lack microsatellites or simple sequence repeat (SSR) markers. Development of genic SSR markers from expressed sequence tagged (EST) database is an alternate and efficient approach to generate the standard DNA markers for genome analysis of such crop species. In the present paper a total of 816 EST-SSRs containing perfect repeats of mono (33.5%), di (14.7%), tri (39.3%), tetra (2.7%), penta (0.7%) and hexa (0.4%) nucleotides were identified from 1,87,763 ESTs of Medicago truncatula. Along with, 70 (8.5%) SSRs of a compound type were also observed. Seven primer pairs of tri repeats were tested for cross transferability in 19 accessions of forage legumes comprising 11 genera. At two different annealing temperatures (55 and 60 degreesC) all primer pairs except AJ410087 reacted with many accessions of forage legumes. Atotal of 51 alleles were detected with six M. truncatula EST-SSRs primer-pairs against DNAfrom 19 accessions representing 11 genera where number of alleles ranged from 2 to 13. The cross-transferability of these EST-SSRs was 40.6% at 55 degreesC and 32.3% at 60 degreesC annealing temperature. 24 alleles of the total 50 (48%) at 55 degreesC and 27 of 51 (53%) at 60 degreesC were polymorphic among the accessions. These 27 polymorphic amplicons identified could be used as DNA markers. This study demonstrates the developed SSR markers from M. truncatula ESTs as a valuable genetic markers and also proposes the possibility of transferring these markers between species of different genera of the legumes of forage importance. It was evident from the results obtained with a set of Desmanthus virgatus accessions where SequentialAgglomerative Hierarchical and Nested (SAHN) cluster analysis based on Dice similarity and Unweighted Pair Group Method with Arithmetic mean Algorithm (UPGMA) revealed significant variability (24 to 74%) among the accessions. High bootstrap values (>30) supported the nodes generated by dendrogram analysis of

  20. Pscroph, a parasitic plant EST database enriched for parasite associated transcripts

    Directory of Open Access Journals (Sweden)

    Tomilov Alexey A

    2005-11-01

    Full Text Available Abstract Background Parasitic plants in the Orobanchaceae develop invasive root haustoria upon contact with host roots or root factors. The development of haustoria can be visually monitored and is rapid, highly synchronous, and strongly dependent on host factor exposure; therefore it provides a tractable system for studying chemical communications between roots of different plants. Description Triphysaria is a facultative parasitic plant that initiates haustorium development within minutes after contact with host plant roots, root exudates, or purified haustorium-inducing phenolics. In order to identify genes associated with host root identification and early haustorium development, we sequenced suppression subtractive libraries (SSH enriched for transcripts regulated in Triphysaria roots within five hours of exposure to Arabidopsis roots or the purified haustorium-inducing factor 2,6 dimethoxybenzoquinone. The sequences of over nine thousand ESTs from three SSH libraries and their subsequent assemblies are available at the Pscroph database http://pscroph.ucdavis.edu. The web site also provides BLAST functions and allows keyword searches of functional annotations. Conclusion Libraries prepared from Triphysaria roots treated with host roots or haustorium inducing factors were enriched for transcripts predicted to function in stress responses, electron transport or protein metabolism. In addition to parasitic plant investigations, the Pscroph database provides a useful resource for investigations in rhizosphere interactions, chemical signaling between organisms, and plant development and evolution.

  1. Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters

    Directory of Open Access Journals (Sweden)

    Siddiqui Asim

    2005-10-01

    Full Text Available Abstract Background The sequencing and analysis of ESTs is for now the only practical approach for large-scale gene discovery and annotation in conifers because their very large genomes are unlikely to be sequenced in the near future. Our objective was to produce extensive collections of ESTs and cDNA clones to support manufacture of cDNA microarrays and gene discovery in white spruce (Picea glauca [Moench] Voss. Results We produced 16 cDNA libraries from different tissues and a variety of treatments, and partially sequenced 50,000 cDNA clones. High quality 3' and 5' reads were assembled into 16,578 consensus sequences, 45% of which represented full length inserts. Consensus sequences derived from 5' and 3' reads of the same cDNA clone were linked to define 14,471 transcripts. A large proportion (84% of the spruce sequences matched a pine sequence, but only 68% of the spruce transcripts had homologs in Arabidopsis or rice. Nearly all the sequences that matched the Populus trichocarpa genome (the only sequenced tree genome also matched rice or Arabidopsis genomes. We used several sequence similarity search approaches for assignment of putative functions, including blast searches against general and specialized databases (transcription factors, cell wall related proteins, Gene Ontology term assignation and Hidden Markov Model searches against PFAM protein families and domains. In total, 70% of the spruce transcripts displayed matches to proteins of known or unknown function in the Uniref100 database (blastx e-value Arabidopsis or rice genomes. Detailed analysis of translationally controlled tumour proteins and S-adenosylmethionine synthetase families confirmed a twofold size difference. Sequences and annotations were organized in a dedicated database, SpruceDB. Several search tools were developed to mine the data either based on their occurrence in the cDNA libraries or on functional annotations. Conclusion This report illustrates specific

  2. EST2uni: an open, parallel tool for automated EST analysis and database creation, with a data mining web interface and microarray expression data integration

    Directory of Open Access Journals (Sweden)

    Nuez Fernando

    2008-01-01

    Full Text Available Abstract Background Expressed sequence tag (EST collections are composed of a high number of single-pass, redundant, partial sequences, which need to be processed, clustered, and annotated to remove low-quality and vector regions, eliminate redundancy and sequencing errors, and provide biologically relevant information. In order to provide a suitable way of performing the different steps in the analysis of the ESTs, flexible computation pipelines adapted to the local needs of specific EST projects have to be developed. Furthermore, EST collections must be stored in highly structured relational databases available to researchers through user-friendly interfaces which allow efficient and complex data mining, thus offering maximum capabilities for their full exploitation. Results We have created EST2uni, an integrated, highly-configurable EST analysis pipeline and data mining software package that automates the pre-processing, clustering, annotation, database creation, and data mining of EST collections. The pipeline uses standard EST analysis tools and the software has a modular design to facilitate the addition of new analytical methods and their configuration. Currently implemented analyses include functional and structural annotation, SNP and microsatellite discovery, integration of previously known genetic marker data and gene expression results, and assistance in cDNA microarray design. It can be run in parallel in a PC cluster in order to reduce the time necessary for the analysis. It also creates a web site linked to the database, showing collection statistics, with complex query capabilities and tools for data mining and retrieval. Conclusion The software package presented here provides an efficient and complete bioinformatics tool for the management of EST collections which is very easy to adapt to the local needs of different EST projects. The code is freely available under the GPL license and can be obtained at http://bioinf.comav.upv.es/est

  3. Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    Yang Jun-Bo

    2010-12-01

    Full Text Available Abstract Background The castor bean (Ricinus communis L., a monotypic species in the spurge family (Euphorbiaceae, 2n = 20, is an important non-edible oilseed crop widely cultivated in tropical, sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid (over 85% in its seed oil, the castor bean seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Due to lack of efficient molecular markers, little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular, expressed sequence tags (ESTs provide valuable resources to develop gene-associated SSR markers. Results In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean. Conclusion Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly

  4. About Libraries - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available acquisition method - Data analysis method Adiantum capillus-veneris EST library creation information Number ...neris EST library About Adiantum capillus-veneris EST library creation information References References of creation

  5. BlastX Result : TrEMBL - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ease 39.9) database. The alignment information of each hit in the Blastx hit list...(release 39.9) database. Link to Uniprot is provided in TogoDB. Align Length The alignment length between th...e above hit sequence and an Adiantum capillus-veneris EST sequence Bit score Score (bit) of the alignment be...tween the above hit sequence and an Adiantum capillus-veneris EST sequence E-value E-value of the alignmen

  6. BlastX Result : Swiss-Prot - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ss-Prot (release 56.9) database. The alignment information of each blast-hit is p...tabase. Link to Uniprot is provided in TogoDB. Align Length The alignment length between the above hit seque...nce and an Adiantum capillus-veneris EST sequence Bit score Score (bit) of the alignment between the above h...it sequence and an Adiantum capillus-veneris EST sequence E-value E-value of the alignment

  7. Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database.

    Directory of Open Access Journals (Sweden)

    Liesbeth Badisco

    Full Text Available BACKGROUND: The desert locust (Schistocerca gregaria displays a fascinating type of phenotypic plasticity, designated as 'phase polyphenism'. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious and gregarious (swarming phase. Although many of the underlying molecular events remain elusive, the central nervous system (CNS is expected to play a crucial role in the phase transition process. Locusts have also proven to be interesting model organisms in a physiological and neurobiological research context. However, molecular studies in locusts are hampered by the fact that genome/transcriptome sequence information available for this branch of insects is still limited. METHODOLOGY: We have generated 34,672 raw expressed sequence tags (EST from the CNS of desert locusts in both phases. These ESTs were assembled in 12,709 unique transcript sequences and nearly 4,000 sequences were functionally annotated. Moreover, the obtained S. gregaria EST information is highly complementary to the existing orthopteran transcriptomic data. Since many novel transcripts encode neuronal signaling and signal transduction components, this paper includes an overview of these sequences. Furthermore, several transcripts being differentially represented in solitarious and gregarious locusts were retrieved from this EST database. The findings highlight the involvement of the CNS in the phase transition process and indicate that this novel annotated database may also add to the emerging knowledge of concomitant neuronal signaling and neuroplasticity events. CONCLUSIONS: In summary, we met the need for novel sequence data from desert locust CNS. To our knowledge, we hereby also present the first insect EST database that is derived from the complete CNS. The obtained S. gregaria EST data constitute an important new source of information that will be instrumental in further unraveling the molecular

  8. Development of a panel of unigene-derived polymorphic EST-SSR markers in lentil using public database information

    Institute of Scientific and Technical Information of China (English)

    Debjyoti Sen Gupta; Peng Cheng; Gaurav Sablok; Dil Thavarajah; Pushparajah Thavarajah; Clarice J Coyne; Shiv Kumar; Michael Baum; Rebecca J McGee

    2016-01-01

    Lentil (Lens culinaris Medik.), a diploid (2n=14) with a genome size greater than 4000 Mbp, is an important cool season food legume grown worldwide. The availability of genomic resources is limited in this crop species. The objective of this study was to develop polymorphic markers in lentil using publicly available curated expressed sequence tag information (ESTs). In this study, 9513 ESTs were downloaded from the National Center for Biotechnology Information (NCBI) database to develop unigene-based simple sequence repeat (SSR) markers. The ESTs were assembled into 4053 unigenes and then analyzed to identify 374 SSRs using the MISA microsatellite identification tool. Among the 374 SSRs, 26 compound SSRs were observed. Primer pairs for these SSRs were designed using Primer3 version 1.14. To classify the functional annotation of ESTs and EST–SSRs, BLASTx searches (using E-value 1 × 10−5) against the public UniProt (http://www.uniprot.org/) and NCBI (http://www.ncbi.nlh.nih.gov/) data-bases were performed. Further functional annotation was performed using PLAZA (version 3.0) comparative genomics and GO annotation was summarized using the Plant GO slim category. Among the synthesized 312 primers, 219 successfully amplified Lens DNA. A diverse panel of 24 Lens genotypes was used to identify polymorphic markers. A polymorphic set of 57 markers successfully discriminated the test genotypes. This set of polymorphic markers with functional annotation data could be used as molecular tools in lentil breeding.

  9. EuDBase: An online resource for automated EST analysis pipeline (ESTFrontier) and database for red seaweed Eucheuma denticulatum.

    Science.gov (United States)

    Hussein, Zeti Azura Mohamed; Loke, Kok Keong; Abidin, Rabiatul Adawiah Zainal; Othman, Roohaida

    2011-01-01

    Functional genomics has proven to be an efficient tool in identifying genes involved in various biological functions. However the availability of commercially important seaweed Eucheuma denticulatum functional resources is still limited. EuDBase is the first seaweed online repository that provides integrated access to ESTs of Eucheuma denticulatum generated from samples collected from Kudat and Semporna in Sabah, Malaysia. The database stored 10,031 ESTs that are clustered and assembled into 2,275 unique transcripts (UT) and 955 singletons. Raw data were automatically processed using ESTFrontier, an in-house automated EST analysis pipeline. Data was collected in MySQL database. Web interface is implemented using PHP and it allows browsing and querying EuDBase through search engine. Data is searchable via BLAST hit, domain search, Gene Ontology or KEGG Pathway. A user-friendly interface allows the identification of sequences either using a simple text query or similarity search. The development of EuDBase is initiated to store, manage and analyze the E. denticulatum ESTs and to provide accumulative digital resources for the use of global scientific community. EuDBase is freely available from http://www.inbiosis.ukm.my/eudbase/.

  10. Physiology and Proteomics of Drought Stress Response in Leaf of Jerusalem Artichoke Seedling

    Institute of Scientific and Technical Information of China (English)

    Meide Zhang; Qiang Chen; Shihua Shen

    2012-01-01

    Drought stress influenced physiological and biochemical process in Jerusalem artichoke (Helianthus tuberosus L.).It caused the decrease of leaf water potential and membrane lipid peroxidation in Jerusalem artichoke.Drought also led to the accumulation of reactive oxygen species (ROS),which could result in the oxidative damage.Two-DE results showed 69 protein spots were changed in cv.Xiuyan.Among them,46 proteins were identified through LC-MS/MS and NCBI database searching.The identified proteins could be sorted into 6 functional categories,including photosynthesis,defense,signal transduction,carbon metabolism,energy and others.Drought stress inhibited expression of some photosynthetic proteins,and also induced the expression of proteins related to defense.In addition,expression of proteins related to signal transduction,carbon metabolism and other functional categories also differed in two cultivars.

  11. Details of rice EST mapping results - RGP estmap2001 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RGP...ne-specific EST primers. Data file File name: rgp_estmap2001_detail.zip File URL:... ftp://ftp.biosciencedbc.jp/archive/rgp-estmap2001/LATEST/rgp_estmap2001_detail.zip File size: 242 KB Simple... search URL http://togodb.biosciencedbc.jp/togodb/view/rgp_estmap2001_detail#en Data acquisition method We u...ontact Us Details of rice EST mapping results - RGP estmap2001 | LSDB Archive ...

  12. SSR mining in oil palm EST database: application in oil palm germplasm diversity studies

    Indian Academy of Sciences (India)

    Ngoot-Chin Ting; Noorhariza Mohd Zaki; Rozana Rosli; Eng-Ti Leslie Low; Maizura Ithnin; Suan-Choo Cheah; Soon-Guan Tan; Rajinder Singh

    2010-08-01

    This study reports on the detection of additional expressed sequence tags (EST) derived simple sequence repeat (SSR) markers for the oil palm. A large collection of 19243 Elaeis guineensis ESTs were assembled to give 10258 unique sequences, of which 629 ESTs were found to contain 722 SSRs with a variety of motifs. Dinucleotide repeats formed the largest group (45.6%) consisting of 66.9% AG/CT, 21.9% AT/AT, 10.9% AC/GT and 0.3% CG/CG motifs. This was followed by trinucleotide repeats, which is the second most abundant repeat types (34.5%) consisting of AAG/CTT (23.3%), AGG/CCT (13.7%), CCG/CGG (11.2%), AAT/ATT (10.8%), AGC/GCT (10.0%), ACT/AGT (8.8%), ACG/CGT (7.6%), ACC/GGT (7.2%), AAC/GTT (3.6%) and AGT/ACT (3.6%) motifs. Primer pairs were designed for 405 unique EST-SSRs and 15 of these were used to genotype 105 E. guineensis and 30 E. oleifera accessions. Fourteen SSRs were polymorphic in at least one germplasm revealing a total of 101 alleles. The high percentage (78.0%) of alleles found to be specific for either E. guineensis or E. oleifera has increased the power for discriminating the two species. The estimates of genetic differentiation detected by EST-SSRs were compared to those reported previously. The transferability across palm taxa to two Cocos nucifera and six exotic palms is also presented. The polymerase chain reaction (PCR) products of three primer-pairs detected in E. guineensis, E. oleifera, C. nucifera and Jessinia bataua were cloned and sequenced. Sequence alignments showed mutations within the SSR site and the flanking regions. Phenetic analysis based on the sequence data revealed that C. nucifera is closer to oil palm compared to J. bataua; consistent with the taxanomic classification.

  13. Succinic acid production from Jerusalem artichoke

    DEFF Research Database (Denmark)

    Gunnarsson, Ingólfur Bragi; Karakashev, Dimitar Borisov; Angelidaki, Irini

    In this work, A. succinogenes 130Z was used to produce succinic acid from Jerusalem artichoke tuber hydrolysate. Results showed that both fructose and glucose in the tuber hydrolysate were utilized for succinic acid production. The sugar utilization was found to be dependent on process control......, hence, when pH was fixed at 6.8 the sugar utilization of fructose was increased from 68.6% to 96.5% and the succinic acid production was also increased by 26.4% to yield 26.8 g/L succinic acid. In this study a one-step pretreatment/hydrolysis method was used where no enzymes were used. Our work suggests...... that Jerusalem artichoke tubers could be utilized for production of bio-succinic acid....

  14. 2-D DIGE analysis of UV-C radiation-responsive proteins in globe artichoke leaves.

    Science.gov (United States)

    Falvo, Sara; Di Carli, Mariasole; Desiderio, Angiola; Benvenuto, Eugenio; Moglia, Andrea; America, Twan; Lanteri, Sergio; Acquadro, Alberto

    2012-02-01

    Plants respond to ultraviolet stress inducing a self-defence through the regulation of specific gene family members. The UV acclimation is the result of biochemical and physiological processes, such as enhancement of the antioxidant enzymatic system and accumulation of UV-absorbing phenolic compounds (e.g. flavonoids). Globe artichoke is an attractive species for studying the protein network involved in UV stress response, being characterized by remarkable levels of inducible antioxidants. Proteomic tools can assist the evaluation of the expression patterns of UV-responsive proteins and we applied the difference in-gel electrophoresis (DIGE) technology for monitoring the globe artichoke proteome variation at four time points following an acute UV-C exposure. A total of 145 UV-C-modulated proteins were observed and 119 were identified by LC-MS/MS using a ∼144,000 customized Compositae protein database, which included about 19,000 globe artichoke unigenes. Proteins were Gene Ontology (GO) categorized, visualized on their pathways and their behaviour was discussed. A predicted protein interaction network was produced and highly connected hub-like proteins were highlighted. Most of the proteins differentially modulated were chloroplast located, involved in photosynthesis, sugar metabolisms, protein folding and abiotic stress. The identification of UV-C-responsive proteins may contribute to shed light on the molecular mechanisms underlying plant responses to UV stress.

  15. Ethanol production from Jerusalem artichoke tubers (Helianthus tuberosus) using Kluyveromyces marxianus and Saccharomyces rosei

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.

    1982-04-01

    This article examines the potential of Jerusalem artichoke as a source for ethanol and single-cell protein SCP. In addition, experimental results are presented on batch fermentation kinetics employing two strains of Kluyveromyces marxianus and one strain of Saccharomyces rosei grown in the extract derived from the tubers of Jeusalem artichoke. Of the three cultures examined, Kluyveromyces marxianus UCD (EST) 55-82 was found to be the best producer of ethanol grown in a simple medium at 35/sup 0/C. The ethanol production was found to be growth-associated haveing a ..mu../sub max/ = 0.41 h/sup -1/ and the ethanol and biomass yields were determined to be Y/sub p///sub = 0.45 (88% of the theoretical) and Y/sub x///sub s/ = 0.04 with 92% of the original sugars utilized. On the basis of carbohydrate yields of Jerusalem artichoke reported in the literature and these batch kinetic studies with K. marxianus, the calculated ethanol yields were found to range from 1400 kg ethanol acre/sup -1/ yr /sup -1/ to a maximum of 2700 kg ethanol acre/sup -1/ yr/sup -1/. The SCP yields for K. marxianus were calculated to range between 130 to 250 kg dry wt cell acre/sup -1/ yr/sup -1/. The potential for developing an integrated process to produce ethanol and SCP is also discussed.

  16. Genetic resources collections of leafy vegetables (lettuce, spinach, chicory, artichoke, asparagus, lamb’s lettuce, rhubarb and rocket salad): composition and gaps

    NARCIS (Netherlands)

    Treuren, van R.; Coquin, P.; Lohwasser, U.

    2012-01-01

    Lettuce, spinach and chicory are generally considered the main leafy vegetables, while a fourth group denoted by ‘minor leafy vegetables’ includes, amongst others, rocket salad, lamb’s lettuce, asparagus, artichoke and rhubarb. Except in the case of lettuce, central crop databases of leafy vegetable

  17. Comparative high-throughput transcriptome sequencing and development of SiESTa, the Silene EST annotation database

    Directory of Open Access Journals (Sweden)

    Marais Gabriel AB

    2011-07-01

    Full Text Available Abstract Background The genus Silene is widely used as a model system for addressing ecological and evolutionary questions in plants, but advances in using the genus as a model system are impeded by the lack of available resources for studying its genome. Massively parallel sequencing cDNA has recently developed into an efficient method for characterizing the transcriptomes of non-model organisms, generating massive amounts of data that enable the study of multiple species in a comparative framework. The sequences generated provide an excellent resource for identifying expressed genes, characterizing functional variation and developing molecular markers, thereby laying the foundations for future studies on gene sequence and gene expression divergence. Here, we report the results of a comparative transcriptome sequencing study of eight individuals representing four Silene and one Dianthus species as outgroup. All sequences and annotations have been deposited in a newly developed and publicly available database called SiESTa, the Silene EST annotation database. Results A total of 1,041,122 EST reads were generated in two runs on a Roche GS-FLX 454 pyrosequencing platform. EST reads were analyzed separately for all eight individuals sequenced and were assembled into contigs using TGICL. These were annotated with results from BLASTX searches and Gene Ontology (GO terms, and thousands of single-nucleotide polymorphisms (SNPs were characterized. Unassembled reads were kept as singletons and together with the contigs contributed to the unigenes characterized in each individual. The high quality of unigenes is evidenced by the proportion (49% that have significant hits in similarity searches with the A. thaliana proteome. The SiESTa database is accessible at http://www.siesta.ethz.ch. Conclusion The sequence collections established in the present study provide an important genomic resource for four Silene and one Dianthus species and will help to

  18. Databases

    Data.gov (United States)

    National Aeronautics and Space Administration — The databases of computational and experimental data from the first Aeroelastic Prediction Workshop are located here. The databases file names tell their contents by...

  19. New cropping designs for globe artichoke industry

    Directory of Open Access Journals (Sweden)

    Rosario Paolo Mauro

    2011-03-01

    Full Text Available A two-year experiment was carried-out in order to evaluate the effects of two plant arrangements (single vs. twin rows and four plant densities (1.0 -1.2 -1.4 and 1.8 plant m–2 on the agronomical behaviour and head characteristics of three globe artichoke genotypes (Violetto di Sicilia, Harmony F1 and Madrigal F1. The change of the cultivation format toward a high density stand significantly increase yield and yield synchronicity. The twin rows plant arrangement, although reduced total yield, increased the yield synchronicity. Moreover, the cultivation of seed-propagated genotypes (Harmony F1 and Madrigal F1 allowed extending significantly the availability of the heads across the year. On the basis of our results, we can assert that the implementation of a specific scheduling cultivation, based on higher density stands, twin rows plant arrangement and the integration of the traditional early genotypes with the new seed-propagated cultivars, is a promising way to match the requirements of a globe artichoke industrial crop, and to predispose a better mechanization of the cultural practices.

  20. Databases

    Directory of Open Access Journals (Sweden)

    Nick Ryan

    2004-01-01

    Full Text Available Databases are deeply embedded in archaeology, underpinning and supporting many aspects of the subject. However, as well as providing a means for storing, retrieving and modifying data, databases themselves must be a result of a detailed analysis and design process. This article looks at this process, and shows how the characteristics of data models affect the process of database design and implementation. The impact of the Internet on the development of databases is examined, and the article concludes with a discussion of a range of issues associated with the recording and management of archaeological data.

  1. EST sequences and their annotation (amino acid sequence and results of homology search) - Dicty_cDB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available and VS) derived from five developmental stages. Clone ID ID of cDNA clone Atlas ID ID of Atlas database ( ht...tp://dictycdb.biol.tsukuba.ac.jp/~tools/bin/ISH/index.html ) and link to Atlas database NBRP ID ID of cDNA c...ir annotations (amino acid sequence, homology search results (with target DBs: dicty EST-DB, DNA-DB and prot...ein-DB)). Links to the Atlas database ( http://dictycdb.biol.tsukuba.ac.jp/~tools/bin/ISH/index.html ), whic

  2. Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

    Science.gov (United States)

    Liu, Shengyi; Shi, Xuejie; Xu, Lanlan; Yi, Yuetao

    2016-03-01

    Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects of processing variables (extraction temperature, pH, extraction time, and liquid-to-solid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: pH 1.52, 63.62 min, 100°C and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52±0.90% was obtained, which validated the model prediction. The effects of diff erent drying methods (freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural diff erences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by diff erent methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin (SDP) and apple pectin (AP) were stronger than those of vacuum oven dried pectin (ODP) and vacuum freeze dried pectin (FDP). Therefore compared with the other two drying methods, the spray drying method was the best.

  3. New Polylactic Acid Composites Reinforced with Artichoke Fibers

    Directory of Open Access Journals (Sweden)

    Luigi Botta

    2015-11-01

    Full Text Available In this work, artichoke fibers were used for the first time to prepare poly(lactic acid (PLA-based biocomposites. In particular, two PLA/artichoke composites with the same fiber loading (10% w/w were prepared by the film-stacking method: the first one (UNID reinforced with unidirectional long artichoke fibers, the second one (RANDOM reinforced by randomly-oriented long artichoke fibers. Both composites were mechanically characterized in tensile mode by quasi-static and dynamic mechanical tests. The morphology of the fracture surfaces was analyzed through scanning electron microscopy (SEM. Moreover, a theoretical model, i.e., Hill’s method, was used to fit the experimental Young’s modulus of the biocomposites. The quasi-static tensile tests revealed that the modulus of UNID composites is significantly higher than that of the neat PLA (i.e., ~40%. Moreover, the tensile strength is slightly higher than that of the neat matrix. The other way around, the stiffness of RANDOM composites is not significantly improved, and the tensile strength decreases in comparison to the neat PLA.

  4. Analysis of expressed sequence tags from Actinidia: applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

    Directory of Open Access Journals (Sweden)

    Richardson Annette C

    2008-07-01

    Full Text Available Abstract Background Kiwifruit (Actinidia spp. are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs. Results The ESTs were derived mainly from four Actinidia species (A. chinensis, A. deliciosa, A. arguta and A. eriantha and fell into 41,858 non redundant clusters (18,070 tentative consensus sequences and 23,788 EST singletons. Analysis of flavor and fragrance-related gene families (acyltransferases and carboxylesterases and pathways (terpenoid biosynthesis is presented in comparison with a chemical analysis of the compounds present in Actinidia including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. Conclusion This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the Actinidia genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in Actinidia.

  5. Molecular properties and prebiotic effect of inulin obtained from artichoke (Cynara scolymus L.).

    Science.gov (United States)

    López-Molina, Dorotea; Navarro-Martínez, María Dolores; Rojas Melgarejo, Francisco; Hiner, Alexander N P; Chazarra, Soledad; Rodríguez-López, José Neptuno

    2005-06-01

    A high molecular weight inulin has been prepared from artichoke (Cynara scolymus L.) agroindustrial wastes using environmentally benign aqueous extraction procedures. Physico-chemical analysis of the properties of artichoke inulin was carried out. Its average degree of polymerization was 46, which is higher than for Jerusalem artichoke, chicory, and dahlia inulins. GC-MS confirmed that the main constituent monosaccharide in artichoke inulin was fructose and its degradation by inulinase indicated that it contained the expected beta-2,1-fructan bonds. The FT-IR spectrum was identical to that of chicory inulin. These data indicate that artichoke inulin will be suitable for use in a wide range of food applications. The health-promoting prebiotic effects of artichoke inulin were demonstrated in an extensive microbiological study showing a long lasting bifidogenic effect on Bifidobacterium bifidum ATCC 29521 cultures and also in mixed cultures of colonic bacteria.

  6. Generation of a predicted protein database from EST data and application to iTRAQ analyses in grape (Vitis vinifera cv. Cabernet Sauvignon berries at ripening initiation

    Directory of Open Access Journals (Sweden)

    Smith Derek

    2009-01-01

    Full Text Available Abstract Background iTRAQ is a proteomics technique that uses isobaric tags for relative and absolute quantitation of tryptic peptides. In proteomics experiments, the detection and high confidence annotation of proteins and the significance of corresponding expression differences can depend on the quality and the species specificity of the tryptic peptide map database used for analysis of the data. For species for which finished genome sequence data are not available, identification of proteins relies on similarity to proteins from other species using comprehensive peptide map databases such as the MSDB. Results We were interested in characterizing ripening initiation ('veraison' in grape berries at the protein level in order to better define the molecular control of this important process for grape growers and wine makers. We developed a bioinformatic pipeline for processing EST data in order to produce a predicted tryptic peptide database specifically targeted to the wine grape cultivar, Vitis vinifera cv. Cabernet Sauvignon, and lacking truncated N- and C-terminal fragments. By searching iTRAQ MS/MS data generated from berry exocarp and mesocarp samples at ripening initiation, we determined that implementation of the custom database afforded a large improvement in high confidence peptide annotation in comparison to the MSDB. We used iTRAQ MS/MS in conjunction with custom peptide db searches to quantitatively characterize several important pathway components for berry ripening previously described at the transcriptional level and confirmed expression patterns for these at the protein level. Conclusion We determined that a predicted peptide database for MS/MS applications can be derived from EST data using advanced clustering and trimming approaches and successfully implemented for quantitative proteome profiling. Quantitative shotgun proteome profiling holds great promise for characterizing biological processes such as fruit ripening

  7. Fuel ethanol production from Jerusalem artichoke stalks using different yeasts

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.; Bajpai, P.K.

    1983-01-01

    The inulin-type sugars present in the stalks of Jerusalem artichoke (Helianthus tuberosus) were extracted with hot water and were used as a substrate to produce fuel EtOH. Seven different yeasts were used to obtain batch kinetic data. The medium consisted of stalk extract from Jerusalem artichoke containing 7.3% total sugars, supplemented with 0.01% oleic acid, 0.01% corn steep liquor, and 0.05% Tween 80. All batch fermentations were carried out in a 1-L bioreactor at 35 degrees and pH 4.6, and the following parameters were measured as a function of time: total sugars, EtOH and biomass concentration, maximum specific growth rate, and biomass and EtOH yields. The best EtOH producer was Kluyveromyces marxianus UCD (FST) 55-82 which gave an EtOH-to-sugar yield 97% of the theoretical maximum value, with almost 100% sugar utilization.

  8. The Possible Efficacy of Artichoke in Fluconazole Related Hepatotoxicity

    Directory of Open Access Journals (Sweden)

    Hüseyin Kurt

    2014-01-01

    Full Text Available Although fluconazole related hepatotoxicity (FRH is rare, mortal acute hepatic necrosis and jaundice were reported in immunocompromised states such as acquired immunodeficiency syndrome (AIDS and bone marrow transplant (BMT. We present a case of a patient with multiple sclerosis who developed hepatotoxicity with the use of a single 150 mg fluconazole tablet for fungal vaginitis, 10 days after methylprednisolone pulse treatment. Our patient’s alanine aminotransferase (ALT and aspartate aminotransferase (AST levels were decreased, 1200 U/L and 800 U/L, respectively, and bilirubin levels were consistent at 37 mg/dL. Artichoke which has anticholestatic and antioxidant properties was used by our patient. She consumed a 30 mg artichoke leaf extract tea 3 times a day. The bilirubin levels significantly declined at the end of the first week and all liver function tests were normalized within 2 months.

  9. Annotation of novel neuropeptide precursors in the migratory locust based on transcript screening of a public EST database and mass spectrometry

    Directory of Open Access Journals (Sweden)

    De Loof Arnold

    2006-08-01

    Full Text Available Abstract Background For holometabolous insects there has been an explosion of proteomic and peptidomic information thanks to large genome sequencing projects. Heterometabolous insects, although comprising many important species, have been far less studied. The migratory locust Locusta migratoria, a heterometabolous insect, is one of the most infamous agricultural pests. They undergo a well-known and profound phase transition from the relatively harmless solitary form to a ferocious gregarious form. The underlying regulatory mechanisms of this phase transition are not fully understood, but it is undoubtedly that neuropeptides are involved. However, neuropeptide research in locusts is hampered by the absence of genomic information. Results Recently, EST (Expressed Sequence Tag databases from Locusta migratoria were constructed. Using bioinformatical tools, we searched these EST databases specifically for neuropeptide precursors. Based on known locust neuropeptide sequences, we confirmed the sequence of several previously identified neuropeptide precursors (i.e. pacifastin-related peptides, which consolidated our method. In addition, we found two novel neuroparsin precursors and annotated the hitherto unknown tachykinin precursor. Besides one of the known tachykinin peptides, this EST contained an additional tachykinin-like sequence. Using neuropeptide precursors from Drosophila melanogaster as a query, we succeeded in annotating the Locusta neuropeptide F, allatostatin-C and ecdysis-triggering hormone precursor, which until now had not been identified in locusts or in any other heterometabolous insect. For the tachykinin precursor, the ecdysis-triggering hormone precursor and the allatostatin-C precursor, translation of the predicted neuropeptides in neural tissues was confirmed with mass spectrometric techniques. Conclusion In this study we describe the annotation of 6 novel neuropeptide precursors and the neuropeptides they encode from the

  10. Influence of planting date and temperature on inulin content in Jerusalem Artichoke (Helianthus tuberosus L.)

    Science.gov (United States)

    Lower temperatures during the dry season in tropical regions might affect inulin content and inulin yield of Jerusalem artichoke. The objective of this study was to determine the effect of planting dates during low temperature on inulin yield and content of Jerusalem artichoke. Two pot experiments...

  11. Artichoke (Cynara scolymus L. as cash-cover crop in an organic vegetable system

    Directory of Open Access Journals (Sweden)

    Anna LENZI

    2015-11-01

    Full Text Available In organic vegetable systems green manure crops play an important role as a nitrogen source, but they cover the soil for several months without producing a direct income. Globe artichoke (Cynara scolymus L. provides both heads to be harvested and particularly abundant plant residues to be possibly incorporated into the soil, so it may play a double role of cash and cover crop. This paper describes an on-farm study in which seed-propagated artichoke, cultivated as an annual crop, preceded zucchini squash and lettuce cultivated in sequence within a vegetable organic system. Artichoke produced about 7 t ha-1 of saleable heads and left, after harvest, 50.3 t ha-1 of fresh biomass usable as green manure. Zucchini squash and lettuce following artichoke showed a significant increase in yield when artichoke residues were incorporated into the soil. Furthermore, a residual positive effect of green manure on soil fertility was detected after lettuce harvest. 

  12. Survey of glycine-rich proteins (GRPs in the Eucalyptus expressed sequence tag database (ForEST

    Directory of Open Access Journals (Sweden)

    Silvia Nora Bocca

    2005-01-01

    Full Text Available The occurrence of quasi-repetitive glycine-rich peptides has been reported in different organisms. Glycine-rich regions are proposed to be involved in protein-protein interactions in some mammalian protein families. In plants, a set of glycine-rich proteins (GRPs was characterized several years ago, and since then a wealth of new GRPs have been identified. GRPs may have very diverse sub-cellular localization and functions. The only common feature among all different GRPs is the presence of glycine-rich repeat domains. The expression of genes encoding GRPs is developmentally regulated, and also induced, in several plant genera, by physical, chemical and biological factors. In addition to the highly modulated expression, several GRPs also show tissue-specific localization. GRPs specifically expressed in xylem, phloem, epidermis, anther tapetum and roots have been described. In this paper, the structural and functional features of these proteins in Eucalyptus are summarized. Since this is the first description of GRPs in this species, particular emphasis has been given to the expression pattern of these genes by analyzing their abundance and prevalence in the different cDNA-libraries of the Eucalyptus Genome Sequencing Project Consortium (ForEST. The comparison of GRPs from Eucalyptus and other species is also discussed.

  13. Putative Vitis vinifera Rop- and Rab-GAP-, GEF-, and GDI-interacting proteins uncovered with novel methods for public genomic and EST database analysis.

    Science.gov (United States)

    Abbal, Philippe; Tesniere, Catherine

    2010-01-01

    To understand how grapevine Rop and Rab proteins achieve their functional versatility in signalling, identification of the putative VvRop- and VvRab-interacting proteins was performed using newly designed tools. In this study, sequences encoding eight full-length proteins for VvRop GTPase-activating proteins (GAPs), five for VvRabGAPs, six for VvRop guanine nucleotide exchange factors (GEFs), one for VvRabGEF, five for VvRop GDP dissociation inhibitors (GDIs), and three for VvRabGDIs were identified. These proteins had a CRIB motif or PH domain, a TBC domain, a PRONE domain, a DENN domain, or GDI signatures, respectively. By bootstrap analysis, an unrooted consensus phylogenetic tree was constructed which indicated that VvRopGDIs and VvRopGEFs--but not VvRopGAP--belonged to the same clade, and that VvRabGEF1 protein was more closely related to VvRopGAPs than to the other putative VvRab-interacting proteins. Twenty-two genes out of 28 encoding putative VvRop- and VvRab-interacting proteins could be located on identified grapevine chromosomes. Generally one gene was anchored on one chromosome, but in some cases up to four genes were located on the same chromosome. Expression patterns of the genes encoding putative VvRop- and VvRab-interacting proteins were also examined using a newly developed tool based on public expressed sequence tag (EST) database analysis. Expression patterns were sometimes found to be specific to an organ or a developmental stage. Although some limitations exist, the use of EST database analysis is stressed, in particular in the case of species where expression data are obtained at high costs in terms of time and effort.

  14. Productivity and fermentability of Jerusalem artichoke according to harvesting date

    Energy Technology Data Exchange (ETDEWEB)

    Chabbert, N.; Arnoux, M.; Braun, Ph.; Galzy, P.; Guiraud, J.P.

    1983-01-01

    The amount of alcohol obtained per hectare of Jerusalem artichoke culture depends on the yield of tubers, the sugar content of the tubers and the fermentability of these sugars. Under Mediterranean climate conditions, the cultivar 'Violet commun' attained its maximum tuber production by 15 November, when the stems and leaves dried up, and then remained constant through the winter. The sugar content of the tubers varied little during this period. However, the sugar composition did vary with time: the polyfructosans were depolymerized. The fermentability of sugars without prior chemical hydrolysis was quite good with Kluyveromyces marxianus which showed high inulinase activity in contrast to Saccharomyces cerevisiae.

  15. Productivity and fermentability of Jerusalem artichoke according to harvesting date

    Energy Technology Data Exchange (ETDEWEB)

    Chabbert, M.; Braunt, Ph.; Guiraud, J.P.; Arnoux, M.; Galzy, P.

    1983-01-01

    The amount of alcohol obtained per hectare of Jerusalem artichoke culture depends on the yield of tubers, the sugar content of the tubers and the fermentability of these sugars. Under Mediterranean climate conditions, the cultivar 'Violet commun' attained its maximum tuber production by 15 November, when the stems and leaves dried up, and then remained constant through the winter. The sugar content of the tubers varied little during this period. However, the sugar composition did vary with time: the polyfructosans were depolymerized. The fermentability of sugars without prior chemical hydrolysis was quite good with Kluyveromyces marxianus which showed high inulinase activity in contrast to Saccharomyces cerevisiae. (Refs. 13).

  16. Productivity and fermentability of Jerusalem artichoke according to harvesting date

    Energy Technology Data Exchange (ETDEWEB)

    Chabbert, N.; Braun, P.; Guiraud, J.P.; Arnoux, M.; Galzy, P.

    1983-01-01

    The amount of alcohol obtained per hectare of Jerusalem artichoke culture depends on the yield of tubers, the sugar content of the tubers and the fermentability of these sugars. Under Mediterranean climate conditions, the cultivar Violet commun attained its maximum tuber production by 15 November, when the stems and leaves dried up, and then remained constant through the winter. The sugar content of the tubers varied little during this period. However, the sugar composition did vary with time: the polyfructosans were depolymerized. The fermentability of sugars without prior chemical hydrolysis was quite good with Kluyveromyces marxianus which showed high inulinase activity in contrast to Saccaromyces cerevisiae. 5 figures, 1 table.

  17. RESPONSE SURFACE METHODOLOGY ANALYSIS OF POLYPHENOL RECOVERY FROM ARTICHOKE WASTE

    Directory of Open Access Journals (Sweden)

    Antonio Zuorro

    2014-01-01

    Full Text Available Large amounts of a solid waste consisting mainly of outer bracts and stems are produced from the industrial processing of artichokes. In this study, the recovery of polyphenols from the two waste components was investigated. Extraction experiments were carried outby an environmentally friendly procedure using aqueous ethanol as solvent. The total polyphenol content, expressed as mg of GAE per g of dry weight, was 10.23±0.68 mg/g for bracts and 16.36±0.85 mg/g for stems. To evaluate the effect of Temperature (T, Extraction time (E and liquid-to-solid Ratio (R on the extraction yields, a central composite design coupled with response surface methodology was used. Under the best conditions (T = 50°C, E = 110.4 min and R = 20 mL g-1, extraction yields between 90 and 93% were obtained. Statistical analysis of the data showed that E was the most influential factor, followed by T and R. Simplified polynomial models were developed to describe the effect of individual factors and their interactions on the extraction yield of polyphenols. Overall, the results of this study support the potential of using artichoke waste as a source of natural phenolic antioxidants and give useful directions on how to improve recovery by proper selection of extraction conditions.

  18. Micropropagation of globe artichoke (Cynara cardunculus L. var. scolymus).

    Science.gov (United States)

    Iapichino, Giovanni

    2013-01-01

    The globe artichoke (Cynara cardunculus L. var. scolymus) is a perennial plant cultivated in the Mediterranean region and the Americas for its edible young flower heads. Although vegetative propagation by offshoots or by "ovoli" (underground dormant axillary buds) has been the primary method of propagation, the potential for the diffusion of diseases and the phenotypic variability can be very high. The propagation of this species by axillary shoot proliferation from in vitro-cultured meristems produces systemic pathogen-free plants and a higher multiplication rate as compared to that obtained by conventional agamic multiplication. Axillary shoot proliferation can be induced from excised shoot apices cultured on Murashige and Skoog agar solidified medium supplemented with various concentrations of cytokinins and auxins, depending on genotype. For the production of virus-free plants, meristems, 0.3-0.8 mm long are excised from shoot apices and surface sterilized. The transfer of artichoke microshoots to a medium lacking cytokinins or with low cytokinin concentration is critical for rooting. Adventitious roots develop within 3-5 weeks after transfer to root induction MS medium containing NAA or IAA at various concentrations. However, in vitro rooting frequency rate is dependent on the genotype and the protocol used. Acclimatization of in vitro microshoots having 3-4 roots is successfully accomplished; plantlets develop new roots in ex vitro conditions and continue to grow.

  19. Artichoke (Cynara scolymus L.) byproducts as a potential source of health-promoting antioxidant phenolics.

    Science.gov (United States)

    Llorach, Rafael; Espín, Juan Carlos; Tomás-Barberán, Francisco A; Ferreres, Federico

    2002-06-05

    The present study reports a fast, economical, and feasible way to extract antioxidant phenolics from artichoke byproducts: raw artichoke (RA), blanched (thermally treated) artichoke (BA), and artichoke blanching waters (ABW). These byproducts represent a huge amount of discarded material in some industries. Two protocols, with possible industrial applicability, based on both methanol and water extractions were used. Phenolic contents (expressed as caffeic acid derivatives) (grams per 100 g of dry extract) were 15.4 and 9.9 for RA when extracted with methanol and water, respectively; 24.3 and 10.3 for BA when extracted with methanol and water, respectively; and finally, 11.3 g of phenolics/100 mL of ABW. Therefore, methanol extracts yielded more phenolics than water extracts, especially when BA byproducts were used. The higher amount of phenolics in BA could be due to the inactivation of polyphenol oxidase (PPO) at the industrial scale (due to blanching process), avoiding PPO-catalyzed oxidation of these phenolics, a phenomenon that could occur in RA byproducts. Artichoke extracts from industrial byproducts showed a high free radical scavenging activity (versus both DPPH* and ABTS*+ radicals) as well as capacity to inhibit lipid peroxidation (ferric thiocyanate method). According to these results, the use of artichoke extracts from industrial byproducts as possible ingredients to functionalize foodstuffs (to decrease lipid peroxidation and to increase health-promoting properties) is suggested.

  20. The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway

    Directory of Open Access Journals (Sweden)

    Bourgaud Frédéric

    2009-03-01

    Full Text Available Abstract Background The leaves of globe artichoke and cultivated cardoon (Cynara cardunculus L. have significant pharmaceutical properties, which mainly result from their high content of polyphenolic compounds such as monocaffeoylquinic and dicaffeoylquinic acid (DCQ, and a range of flavonoid compounds. Results Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase (HQT encoding genes have been isolated from both globe artichoke and cultivated cardoon (GenBank accessions DQ915589 and DQ915590, respectively using CODEHOP and PCR-RACE. A phylogenetic analysis revealed that their sequences belong to one of the major acyltransferase groups (anthranilate N-hydroxycinnamoyl/benzoyltransferase. The heterologous expression of globe artichoke HQT in E. coli showed that this enzyme can catalyze the esterification of quinic acid with caffeoyl-CoA or p-coumaroyl-CoA to generate, respectively, chlorogenic acid (CGA and p-coumaroyl quinate. Real time PCR experiments demonstrated an increase in the expression level of HQT in UV-C treated leaves, and established a correlation between the synthesis of phenolic acids and protection against damage due to abiotic stress. The HQT gene, together with a gene encoding hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT previously isolated from globe artichoke, have been incorporated within the developing globe artichoke linkage maps. Conclusion A novel acyltransferase involved in the biosynthesis of CGA in globe artichoke has been isolated, characterized and mapped. This is a good basis for our effort to understand the genetic basis of phenylpropanoid (PP biosynthesis in C. cardunculus.

  1. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats

    DEFF Research Database (Denmark)

    Jimenez-Escrig, A.; Dragsted, Lars Ove; Daneshvar, Bahram;

    2003-01-01

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2......,2-diphenyl-1-picrylhydrazyl (DPPH.) scavenging, (b) ferric-reducing antioxidant power (FRAP), and...

  2. Kombucha fermentation on raw extracts of different cultivars of Jerusalem artichoke

    Directory of Open Access Journals (Sweden)

    Lončar Eva S.

    2007-01-01

    Full Text Available Kombucha is a symbiosis between yeasts and acetic bacteria. It usually grows on sweetened black tea, but cultivation is possible on many other substrates. Jerusalem artichoke tubers extract is one of them. Tubers are suitable for the dietetic nutrition because of the low monosaccharide content and presence of some polyfructan ingredients which act as prebiotic. Five different cultivars of Jerusalem artichoke were used for the preparation of substrates for kombucha fermentation. The aim of this paper was the investigation of the influence of different Jerusalem artichoke cultivars on metabolic activity of kombucha. Composition of carbohydrates was followed using thin-layer chromatography and pH, reducing sugars content and yield of biomass were measured. Most of the samples with Jerusalem artichoke tubers extract contained fructose, probably small amount of glucose, fructo-oligosaccharides with different degree of polymerization and, inulin. Considering TLC chromatograms, Jerusalem artichoke cultivar did not affect significantly the composition of oligosaccharides in the fermentative liquid, as only minor differences were observed.

  3. Synbiotic functional drink from Jerusalem artichoke juice fermented by probiotic Lactobacillus plantarum PCS26

    OpenAIRE

    Dimitrovski, Darko; Velickova, Elena; Dimitrovska, Maja; Langerholc, Tomaz; Winkelhausen, Eleonora

    2015-01-01

    A probiotic strain Lactobacillus plantarum PCS26 was used to ferment Jerusalem artichoke juice. Growth kinetics of the bacterial strain was followed during juice fermentation both in flask and in laboratory fermentor. Jerusalem artichoke showed to be an excellent source of nutrients for L. plantarum PCS26 growth. The culture grew very well reaching more than 1010 cfu/ml in just 12 h. The pH changed from the initial 6.5 to 4.6 at the end of fermentation. The culture hydrolyzed fructooligosacch...

  4. Globe Artichoke Callus as an Alternative System for the Production of Dicaffeoylquinic Acids

    NARCIS (Netherlands)

    Moglia, A.; Menin, B.; Comino, C.; Lanteri, S.; Beekwilder, M.J.

    2012-01-01

    Globe artichoke leaves are highly rich in phenolic acids, in particular chlorogenic acid and dicaffeoylquinic acids. The latter are of particular interest since they can exert a stronger antioxidant activity, due to the presence of two adjacent hydroxyl groups on each of their phenolic rings. Plant

  5. CHANGE OF INULIN IN THE TUBERS OF JERUSALEM ARTICHOKE AT STORAGE

    Directory of Open Access Journals (Sweden)

    Nazarenko M. N.

    2013-12-01

    Full Text Available This article examines the change of the mass fraction of inulin in Jerusalem artichoke tubers during storage under different conditions. The influence of temperature, storage time and variety features on the content of inulin have been shown. The terms and conditions of storage of raw materials before processing have been set.

  6. Cadmium Accumulation and Translocation in Two Jerusalem Artichoke (Helianthus tuberosus L.) Cultivars

    Institute of Scientific and Technical Information of China (English)

    CHEN Liang; LONG Xiao-Hua; ZHANG Zhen-Hua; ZHENG Xiao-Tao; Z. RENGEL; LIU Zhao-Pu

    2011-01-01

    Jerusalem artichoke (Helianthus tuberosus L.) not just can be used for bioethanol production but may be potentially used in phytoremediation for the removal of heavy metal pollutants.Two Jerusalem artichoke cultivars,N2 and N5,were subjected to six cadmium (Cd) concentrations (0,5,25,50,100 and 200 mg L-1) to investigate Cd tolerance and accumulation.After 21 days of growth,the effects of Cd on growth,chlorophyll content,net photosynthetic rate,intercellular CO2 concentration and malondialdehyde content were evaluated.Most growth parameters were reduced under Cd stress.The two Jerusalem artichoke cultivars had relatively high Cd tolerance and accumulation capacity (> 100 mg kg-1),with N5 being more tolerant and having higher Cd accumulation than N2.Roots accumulated more Cd than stems and leaves.The bioconcentration factors (far higher than 1) and translocation factors (lower than 1) decreased with an increase in Cd applied.The results suggested that Jerusalem artichoke could be grown at relatively high Cd loads,and N5 could be an excellent candidate for phytoremediation of Cd-contaminated soils.

  7. Effective protein extraction protocol for proteomics studies of Jerusalem artichoke leaves.

    Science.gov (United States)

    Zhang, Meide; Shen, Shihua

    2013-07-01

    Protein extraction is a crucial step for proteomics studies. To establish an effective protein extraction protocol suitable for two-dimensional electrophoresis (2DE) analysis in Jerusalem artichoke (Helianthus tuberosus L.), three different protein extraction methods-trichloroacetic acid/acetone, Mg/NP-40, and phenol/ammonium acetate-were evaluated using Jerusalem artichoke leaves as source materials. Of the three methods, trichloroacetic acid/acetone yielded the best protein separation pattern and highest number of protein spots in 2DE analysis. Proteins highly abundant in leaves, such as Rubisco, are typically problematic during leaf 2DE analysis, however, and this disadvantage was evident using trichloroacetic acid/acetone. To reduce the influence of abundant proteins on the detection of low-abundance proteins, we optimized the trichloroacetic acid/acetone method by incorporating a PEG fractionation approach. After optimization, 363 additional (36.2%) protein spots were detected on the 2DE gel. Our results suggest that trichloroacetic acid/acetone method is a better protein extraction technique than Mg/NP-40 and phenol/ammonium acetate in Jerusalem artichoke leaf 2DE analysis, and that trichloroacetic acid/acetone method combined with PEG fractionation procedure is the most effective approach for leaf 2DE analysis of Jerusalem artichoke.

  8. Design and Simulation of Two Robotic Systems for Automatic Artichoke Harvesting

    Directory of Open Access Journals (Sweden)

    Domenico Longo

    2013-12-01

    Full Text Available The target of this research project was a feasibility study for the development of a robot for automatic or semi-automatic artichoke harvesting. During this project, different solutions for the mechanical parts of the machine, its control system and the harvesting tools were investigated. Moreover, in cooperation with the department DISPA of University of Catania, different field structures with different kinds of artichoke cultivars were studied and tested. The results of this research could improve artichoke production for preserves industries. As a first step, an investigation on existing machines has been done. From this research, it has been shown that very few machines exist for this purpose. Based also on previous experiences, some proposals for different robotic systems have been done, while the mobile platform itself was developed within another research project. At the current stage, several different configurations of machines and harvesting end-effectors have been designed and simulated using a 3D CAD environment interfaced with Matlab®. Moreover, as support for one of the proposed machines, an artificial vision algorithm has been developed in order to locate the artichokes on the plant, with respect to the robot, using images taken with a standard webcam.

  9. In Vitro Callogenesis and Agrobacterium-Mediated Transformation of Globe Artichoke

    NARCIS (Netherlands)

    Menin, B.; Moglia, A.; Comino, C.; Lanteri, S.; Herpen, van T.W.J.M.; Beekwilder, M.J.

    2012-01-01

    Micropropagation techniques have been widely applied in globe artichoke (C. cardunculus L. var. scolymus), however, efficient protocols for the establishment of in vitro callogenesis and organogenesis, a pre-requisite for Agrobacterium-mediated genetic transformation, have not been set up so far. We

  10. El potencial del topinambur en la salud y la nutrición The potential of Jerusalem Artichokes in health and nutrition

    Directory of Open Access Journals (Sweden)

    D Scollo

    2011-12-01

    Full Text Available Introducción: El topinambur es un tubérculo con alto contenido en inulina. La inulina es un carbohidrato soluble no digerible que está presente en muchos vegetales, frutas y cereales siendo usada ampliamente como ingrediente en alimentos funcionales. Objetivo: Obtener tubérculos de topinambur, fijar distintos parámetros de extracción de inulina y preparar diversos alimentos, reemplazando parte de las harinas tradicionales por harina de topinambur. Se propuso, además, evaluar la preferencia de los consumidores sobre los mismos. Material y métodos: Se sembraron y cosecharon tubérculos de topinambur partiendo de 4 parcelas con diferentes condiciones de riego y fertilización. Los mismos se conservaron a variadas temperaturas. Se determinó el contenido de inulina por HPLC y se realizó la extracción de la misma de los tubérculos. A partir de los tubérculos cosechados en la parcela 3 se elaboraron harinas que fueron utilizadas para reemplazar parcialmente a la harina de trigo en la preparación de diferentes productos alimenticios. Sobre los mismos se realizó una evaluación de preferencia. Resultados: El rinde de la cosecha fue mayor en la parcela 3 (tierra fértil y riego según necesidades, 91 ton/Ha. El método de conservación más adecuado fue el frío, sin embargo no se utilizaron estos tubérculos para la extracción de inulina. Los productos alimenticios obtenidos fueron considerados adecuados por los jueces intervinientes. Conclusiones: la elaboración de productos alimenticios a partir de harina de topinambur podría ser una alternativa de alimento funcional por su contenido de inulina.Introduction: The Jerusalem artichoke Is a tuber high in inulin. Inulin is a soluble non-digestible carbohydrate that is present in many vegetables, fruits and cereals and is widely used as an ingredient in functional foods. Objective: To get Jerusalem artichoke tubers, set various parameters for extraction of inulin and prepare various foods

  11. Synbiotic functional drink from Jerusalem artichoke juice fermented by probiotic Lactobacillus plantarum PCS26.

    Science.gov (United States)

    Dimitrovski, Darko; Velickova, Elena; Dimitrovska, Maja; Langerholc, Tomaz; Winkelhausen, Eleonora

    2016-01-01

    A probiotic strain Lactobacillus plantarum PCS26 was used to ferment Jerusalem artichoke juice. Growth kinetics of the bacterial strain was followed during juice fermentation both in flask and in laboratory fermentor. Jerusalem artichoke showed to be an excellent source of nutrients for L. plantarum PCS26 growth. The culture grew very well reaching more than 10(10) cfu/ml in just 12 h. The pH changed from the initial 6.5 to 4.6 at the end of fermentation. The culture hydrolyzed fructooligosaccharides present in the Jerusalem artichoke juice, yielding fructose which was presumably consumed along with the malic acid as energy and carbon source. Lactic acid was the main metabolite produced in concentration of 4.6 g/L. Acetic and succinic acid were also identified. Sensory evaluation of the fermented Jerusalem artichoke juice and its mixtures with blueberry juice showed that the 50/50 % v/v mixture would be very well accepted by the consumers. Above 80 % of the panelists would buy this drink, and over 60 % were willing to pay more for it. Culture survivability in the fermented juices during storage at 4-7 °C was assayed by the Weibullian model. The product shelf-life was extended from 19.70 ± 0.50 days of pure Jerusalem artichoke juice to 35.7 ± 6.4 days of the mixture containing 30 % blueberry juice.

  12. Variation of Phenolic Content in Globe Artichoke in Relation to Biological, Technical and Environmental Factors

    Directory of Open Access Journals (Sweden)

    Giovanni Mauromicale

    2011-02-01

    Full Text Available In Italy, globe artichoke production is prevailingly concentrated in the South and islands, where it provides an important contribution to the agricultural economy. In recent years, there has been a renewed interest in this crop as a promising source of polyphenols, a heterogeneous class of secondary metabolites characterized by various healthy properties well-documented in literature. The phenolic fraction, present in the different artichoke plant parts, varies widely in relation to biotic and abiotic factors. Therefore, the present study aimed at evaluating the variation of phenolic content in globe artichoke in relation to biological, technical and environmental factors. Two field-experiments were carried out in Sicily (South Italy in two representative cultivation areas, in order to examine the effects of genotype, head fraction, season conditions, planting density and arrangement on the globe artichoke phenolic concentration. Both the total polyphenols and the individual phenolic compounds detected were notably genotype- dependent. Particularly, the high level of caffeoylquinic acids (chlorogenic acid, among others and apigenin 7- O-glucuronide, reported respectively by “Violetto di Sicilia” and “Romanesco clone C3”, could be used to encourage globe artichoke fresh consumption. Total polyphenols content also resulted more abundant in specific accumulation sites within the inflorescence, such as the floral stem and receptacle, and for most of genotypes it decreased during the second year in response to the different meteorological conditions. Additionally, total polyphenols content significantly and linearly increased as plant density increased from 1.0 to 1.8 plant m-2 and it significantly increased by 13% passing from single to twin rows plant arrangement.

  13. The effect of culinary preparation on carbohydrate composition, texture and sensory quality of Jerusalem artichoke tubers (Helianthus tuberosus L.)

    DEFF Research Database (Denmark)

    Bach, Vibe; Bennedbæk-Jensen, Sidsel; Kidmose, Ulla;

    2013-01-01

    The Jerusalem artichoke (Helianthus tuberosus L.) tuber is a root vegetable with excellent gastronomic qualities, however the culinary properties are underexploited. Carbohydrate content, instrumental texture analysis and sensory profiling were used to study the effects of culinary preparation in...

  14. Chemical and functional properties of the different by-products of artichoke (Cynara scolymus L.) from industrial canning processing.

    Science.gov (United States)

    Ruiz-Cano, Domingo; Pérez-Llamas, Francisca; Frutos, María José; Arnao, Marino B; Espinosa, Cristóbal; López-Jiménez, José Ángel; Castillo, Julián; Zamora, Salvador

    2014-10-01

    In this study, the basic chemical composition and functional properties of six by-product fractions collected from different steps of artichoke industrial processing were evaluated. Fractions differed in thermal treatment, the bract position in the artichoke head and the cutting size. Contents of moisture, ash, protein, fat, dietary fibre, inulin, total phenolics, total flavonoids, caffeoyl derivatives and flavones were analysed. Antioxidant activity values were also determined. All assessed artichoke by-product fractions contained high-dietary fibre (53.6-67.0%) and low fat (2.5-3.7%). Artichoke by-product fractions contained high levels of inulin, especially in the boiled inner bracts (30%). Total phenolic and flavonoid contents and antioxidant activity (153-729 μmol gallic acid equivalents, 6.9-19.2 μmol quercetin equivalents and 85-234 μmol ascorbic acid equivalents per gram of dry matter, respectively) varied widely with the bract positions in the artichoke head and the thermal treatments. The more interesting fractions for use as functional ingredients were those situated closer to the artichoke heart and thermally treated.

  15. Development of Novel, Exon-Primed Intron-Crossing (EPIC Markers from EST Databases and Evaluation of their Phylogenetic Utility in Commiphora (Burseraceae

    Directory of Open Access Journals (Sweden)

    Morgan R. Gostel

    2014-03-01

    Full Text Available Premise of the study: Novel nuclear exon-primed intron-crossing (EPIC markers were developed to increase phylogenetic resolution among recently diverged lineages in the frankincense and myrrh family, Burseraceae, using Citrus, Arabidopsis, and Oryza genome resources. Methods and Results: Primer pairs for 48 nuclear introns were developed using the genome resource IntrEST and were screened using species of Commiphora and other Burseraceae taxa. Four putative intron regions (RPT6A, BXL2, mtATP Synthase D, and Rab6 sequenced successfully for multiple taxa and recovered phylogenies consistent with those of existing studies. In some cases, these regions yielded informative sequence variation on par with that of the nuclear ribosomal DNA internal transcribed spacer. Conclusions: The combination of freely available genome resources and our design criteria have uncovered four single-copy nuclear intron regions that are useful for phylogenetic reconstruction of Burseraceae taxa. Because our EPIC primers also amplify Arabidopsis, we recommend their trial in other rosid and eudicot lineages.

  16. Extraction, degree of polymerization determination and prebiotic effect evaluation of inulin from Jerusalem artichoke.

    Science.gov (United States)

    Li, Wancong; Zhang, Jun; Yu, Chunwei; Li, Qing; Dong, Fang; Wang, Gang; Gu, Guodong; Guo, Zhanyong

    2015-05-05

    The tubers of Jerusalem artichoke are rich of inulin, which makes the plant one of primary inulin resources in China. The aim of this study was to extract inulin from tubers and test the degree of polymerization (DP) 10 days before flowering to 80 days after flowering. The DP of inulin reaches a maximum of 19 at 50 days after flowering. The variation tendencies of inulin content and DP were almost the same, which increase rapidly at the beginning and then decrease gradually at a lower speed. Meanwhile, the effects of inulin on probiotics in yogurt have been evaluated. It indicated that inulin with low DP has higher activities. Experimental data improve the understanding of status change of inulin in whole growth of Jerusalem artichoke tubers in Northeastern China and are instructive to get inulin with different properties.

  17. Characterization and purification of polyphenol oxidase from artichoke (Cynara scolymus L.).

    Science.gov (United States)

    Dogan, Serap; Turan, Yusuf; Ertürk, Hatibe; Arslan, Oktay

    2005-02-09

    In this study, the polyphenol oxidase (PPO) of artichoke (Cynara scolymus L.) was first purified by a combination of (NH(4))(2)SO(4) precipitation, dialysis, and a Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity column. At the end of purification, 43-fold purification was achieved. The purified enzyme migrated as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Polyacrylamide gel electrophoresis indicated that PPO had a 57 kDa molecular mass. Second, the contents of total phenolic and protein of artichoke head extracts were determined. The total phenolic content of artichoke head was determined spectrophotometrically according to the Folin-Ciocalteu procedure and was found to be 425 mg 100 g(-1) on a fresh weight basis. Protein content was determined according to Bradford method. Third, the effects of substrate specificity, pH, temperature, and heat inactivation were investigated on the activity of PPO purified from artichoke. The enzyme showed activity to 4-methylcatechol, pyrogallol, catechol, and L-dopa. No activity was detected toward L-tyrosine, resorsinol, and p-cresol. According to V(max)/K(m) values, 4-methylcatechol (1393 EU min(-1) mM(-1)) was the best substrate, followed by pyrogallol (1220 EU min(-1) mM(-1)), catechol (697 EU min(-1) mM(-1)), and L-dopa (102 EU min(-1) mM(-1)). The optimum pH values for PPO were 5.0, 8.0, and 7.0 using 4-methylcatechol, pyrogallol, and catechol as substrate, respectively. It was found that optimum temperatures were dependent on the substrates studied. The enzyme activity decreased due to heat denaturation of the enzyme with increasing temperature and inactivation time for 4-methylcatechol and pyrogallol substrates. However, all inactivation experiments for catechol showed that the activity of artichoke PPO increased with mild heating, reached a maximum, and then decreased with time. Finally, inhibition of artichoke PPO was investigated with inhibitors such as L-cysteine, EDTA, ascorbic

  18. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L.) in a Biorefinery Concept

    DEFF Research Database (Denmark)

    Johansson, Eva; Prade, Thomas; Angelidaki, Irini

    2015-01-01

    Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L.) is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper...... focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals...... bioactive activity was found in the young leaves of the crop, and the sesquiterpene lactones are of specific interest, as other compounds from this group have shown inhibitory effects on several human diseases. Thus, future focus should be on understanding the usefulness of small molecules, to develop...

  19. More than multiple introductions: Multiple taxa contribute to the genesis of the invasive California's wild artichoke thistle

    Institute of Scientific and Technical Information of China (English)

    Janet LEAK-GARCIA; Jodie S.HOLT; Seung-Chul KIM; Lisa MU; José A.MEJ(I)AS; Norman C.ELLSTRAND

    2013-01-01

    The history of some invasive species is so complex that their origins can be difficult to determine.One example of such invasive species is the California invasive known as "wild artichoke thistle" (Cynara cardunculus var.sylvestris),found in natural and disturbed ecosystems.Wild artichoke thistle is a Mediterranean native and the progenitor of two domesticated horticultural taxa,artichoke and cardoon.Different hypotheses regarding the origins of California plants have included introductions by 19th century Italian immigrants and the de-domestication (evolutionary reversion to wild-type morphology) of feral (escaped,free-living) cultivars.Using microsatellite markers,we compared the genetic constitutions of 12 artichoke thistle populations in California with possible progenitor populations:17 Spanish and Italian wild populations and eight different artichoke and cardoon cultivars.Each California population was compared with its putative progenitors using STRUCTURE analysis.Our results suggest that California's artichoke thistle populations are polyphyletic.Surprisingly,two-thirds of California's populations closely matched populations from the Iberian Peninsula.Three populations matched domesticated artichoke.One population appears to have wild and cultivar hybrid ancestry.Alleles specific to Italian populations were found at low frequencies in some California plants,suggesting that Italian wild plants may have been in California,but have left a trivial genetic legacy.Given that the de-domesticated plants in this study appear to be as invasive as the wild taxon,we conclude with a discussion of the role that ferality and de-domestication may have in plant invasions.

  20. Studies on Fermentation of Jerusalem artichoke Juice by Beneficial Lactic Acid Bacteria and the Flavor Compounds of Fermented Jerusalem artichoke Juice%乳酸菌发酵菊芋汁及其风味的研究

    Institute of Scientific and Technical Information of China (English)

    李信; 董英; 程新; 刘崇万

    2012-01-01

    对不同品种和产地的菊芋主要成分进行了测定,并利用乳酸菌对菊芋汁进行发酵,研究了不同乳酸菌在菊芋汁中的生长规律、低温存活性及发酵菊芋汁中主要风味物质。结果表明,4种乳酸菌在菊芋汁中均生长良好,最高活菌数可达到10^9CFU/mL;菊芋汁经乳酸菌发酵后具有良好风味,其风味的差异与发酵菌种有关;发酵菊芋汁中乳酸菌在4℃低温贮藏过程中具有较好的低温存活性,4周后活菌数保持在10^9CFU/mL。菊芋汁适用于开发成新的功能性乳酸菌饮料。%Jerusalem artichoke possesses abundant inulin, high content of potassium and low content of sodium. The main nutritional compositions of Jerusalem artichoke from diverse varieties and regions were analyzed. The main purpose of this study was to investigate the growth of four lactic acid bacteria (Lactobacillus plantarum, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus brevis) on Jerusalem artichoke juice and the production of flavor com- pounds in these bacteria. Growth rule, low-temperature survivability of lactic acid bacteria in Jerusalem artichoke and the major flavor compounds of fermented Jerusalem artichoke juice were considered in this study. In this research it was found that four lactic cultures grew well on the Jerusalem artichoke juice, and the viable cell counts of lactic acid bacteria reached 10^9CFU/mL. Jerusalem artichoke juice that was fermented by beneficial lactic acid bacteria had a good flavor, the main flavor compounds were diverse due to the different species of lactic acid bacteria. Although the lactic cultures of fermented Jerusalem artichoke juice gradually lost their viability during cold storage, the viable cell counts of these lactic acid bacteria still remained at 10^8CFU/mL after 4 weeks of refrigeration at 4℃. The results of this study showed that Jerusalem artichoke juice was a potential substrate to be used for healthy

  1. Ethanol production from Jerusalem artichoke tubers (Helianthus tuberosus). Using Kluyveromyces marxcianus and Saccharomyces rosei

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.

    1982-04-01

    This article examines the potential of Jerusalem artichoke as a source for ethanol and single-cell protein SCP. In addition, experimental results are presented on batch fermentation kinetics employing two strains of Kluyveromyces marxianus and one strain of Saccharomyces rosei grown on the extract derived from the tubers of Jerusalem artichoke. Of the three cultures examined, Kluyveromyces marxianus UCD (FST) 55-82 was found to be the best producer of ethanol grown in a simple medium at 35 degrees C. The ethanol production was found to be growth-associated having a mu max = 0.41/h and the ethanol and biomass yields were determined to be Y p/s = 0.45 (88% of the theoretical) and Y x/s = 0.04 with 92% of the original sugars utilized. On the basis of carbohydrate yields of Jerusalem artichoke reported in the literature and these batch kinetic studies with Kluyveromyces marxianus, the calculated ethanol yields were found to range from 1400 kg ethanol/acre/yr to a maximum of 2700 kg ethanol/acre/yr. The SCP yields for Kluyveromyces marxianus were calculated to range between 130 to 250 kg dry wt cell/acre/yr. The potential for developing an integrated process to produce ethanol and SCP is also discussed. (Refs. 27).

  2. Preparation of high-fructose syrup from the tubers of the Jerusalem artichoke (Helianthus tuberosus L.

    Science.gov (United States)

    Fleming, S E; GrootWassink, J W

    1979-11-01

    Fructose has recently received much attention due to renewed interest in natural sweeteners. In addition, fructose has some advantages to sucrose in sweetness, solubility, viscosity, and dental health characteristics. Fructose is deposited as storage fructans of the inulin (beta-1,2) type in tubers and rhizomes of the Compositae family. The utilization of the Jerusalem artichoke (Helianthus tuberosus) tuber as a source of fructose syrup is discussed. This plant has the potential to produce more sugar per acre than corn or sugar beets. In addition, the artichoke has higher frost resistance and lower heat unit requirements than corn and is somewhat more tolerant to low moisture conditions than sugar beets. A high quality fructose syrup can be produced from artichoke tubers. The extraction step was found to be particularly important since development of adverse colors and flavors must be prevented. The fructans may be acid or enzyme hydrolyzed but the latter method gave a higher quality syrup. Ion-exchange resins and activated charcoal were effective in removing coloring and flavoring materials, and also reduced other noncarbohydrate constituents. Since the enzymatic hydrolysis of the fructans is an attractive alternative to acid hydrolysis, a process was developed for producing and purifying a special beta-fructofuranosidase (inulase) from Saccharomyces fragilis. Inulase has a much higher specificity for fructans than commerically available beta-fructofuranosidase (invertase).

  3. Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Nan [Agricultural Univ., Qingdao, SD (China). College of Animal Science and Technology; Chinese Academy of Sciences, Qingdao, SD (China). Key Lab. of Biofuels; Yuan, Bo; Wang, Shi-An; Li, Fu-Li [Chinese Academy of Sciences, Qingdao, SD (China). Key Lab. of Biofuels; Sun, Juan [Agricultural Univ., Qingdao, SD (China). College of Animal Science and Technology

    2012-09-15

    Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L{sup -1}) at 40 C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L{sup -1}, which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP. (orig.)

  4. Carbon sequestration and Jerusalem artichoke biomass under nitrogen applications in coastal saline zone in the northern region of Jiangsu, China.

    Science.gov (United States)

    Niu, Li; Manxia, Chen; Xiumei, Gao; Xiaohua, Long; Hongbo, Shao; Zhaopu, Liu; Zed, Rengel

    2016-10-15

    Agriculture is an important source of greenhouse gases, but can also be a significant sink. Nitrogen fertilization is effective in increasing agricultural production and carbon storage. We explored the effects of different rates of nitrogen fertilization on biomass, carbon density, and carbon sequestration in fields under the cultivation of Jerusalem artichoke as well as in soil in a coastal saline zone for two years. Five nitrogen fertilization rates were tested (in guream(-2)): 4 (N1), 8 (N2), 12 (N3), 16 (N4), and 0 (control, CK). The biomass of different organs of Jerusalem artichoke during the growth cycle was significantly higher in N2 than the other treatments. Under different nitrogen treatments, carbon density in organs of Jerusalem artichoke ranged from 336 to 419gCkg(-1). Carbon sequestration in Jerusalem artichoke was higher in treatments with nitrogen fertilization compared to the CK treatment. The highest carbon sequestration was found in the N2 treatment. Soil carbon content was higher in the 0-10cm than 10-20cm layer, with nitrogen fertilization increasing carbon content in both soil layers. The highest soil carbon sequestration was measured in the N2 treatment. Carbon sequestration in both soil and Jerusalem artichoke residue was increased by nitrogen fertilization depending on the rates in the coastal saline zone studied.

  5. The use of dry Jerusalem artichoke as a functional nutrient in developing extruded food with low glycaemic index.

    Science.gov (United States)

    Radovanovic, Ana; Stojceska, Valentina; Plunkett, Andrew; Jankovic, Slobodan; Milovanovic, Dragan; Cupara, Snezana

    2015-06-15

    This study considers the use of dry Jerusalem artichoke (JA) as a functional nutrient in developing food products with enhanced nutritional characteristics and low glycaemic index (GI). Three different formulations based on buckwheat and JA were developed and processed using extrusion technology. Nutritional properties including the levels of total dietary fibre (TDF), protein, inulin, total carbohydrates and lipids were analysed. A clinical study was performed on ten healthy volunteers (aged between 21 and 56) to determine the level of GI and glycaemic load (GL). The results revealed that JA significantly (Pfood and extruded products, GI and GL. Samples containing 80% of Jerusalem artichoke were considered as a low GI food whilst samples containing 30% and 60% of Jerusalem artichoke as a medium GI food. A similar trend was seen in terms of GL.

  6. Balance between salt stress and endogenous hormones influence dry matter accumulation in Jerusalem artichoke.

    Science.gov (United States)

    Shao, Tianyun; Li, Lingling; Wu, Yawen; Chen, Manxia; Long, Xiaohua; Shao, Hongbo; Liu, Zhaopu; Rengel, Zed

    2016-10-15

    Salinity is one of the most serious environmental stresses limiting agricultural production. Production of Jerusalem artichoke on saline land is strategically important for using saline land resources. The interaction between plant hormones and salinity stress in governing Jerusalem artichoke (Helianthus tuberosus) growth is unclear. Jerusalem artichoke (variety Nanyu-1) was grown under variable salinity stress in the field, and a role of endogenous hormones [zeatin (ZT), auxins (IAA), gibberellins (GA3) and abscisic acid (ABA)] in regulating sugar and dry matter accumulation in tubers was characterized. Under mild salt stress (≤2.2gNaClkg(-1) soil), Nanyu-1 grew well with no significant alteration of dry matter distribution to stems and tubers. In contrast, under moderate salt stress (2.7gNaClkg(-1) soil), the distribution to stem decreased and to tubers decreased significantly. Mild salt stress induced sugar accumulation in tubers at the beginning of the tuber-expansion period, but significantly inhibited (i) transfer of non-reducing sugars to tubers, and (ii) polymerization and accumulation of fructan during the tuber-expansion stage. Under different salinity stress, before the stolon growth, the ratio of IAA/ABA in leaves increased significantly and that of GA3/ABA increased slightly; during tuber development, these ratios continued to decrease and reached the minimum late in the tuber-expansion period. While, salt stress inhibited (i) underground dry matter accumulation, (ii) tuber dry matter accumulation efficiency, (iii) transport of non-reducing sugars to tubers, and (iv) fructan accumulation efficiency during the tuber-expansion period; these effects were accompanied by significantly decreased tuber yield with an increase in salinity. With soil salinity increasing, the synthesis of IAA and GA3 was inhibited in leaves and tubers, while ABA synthesis was stimulated. In brief, tuber yield would significantly decreased with the increase of salinity.

  7. Nitrogen and sugar content variability in tubers of Jerusalem artichoke (Helianthus tuberosus

    Directory of Open Access Journals (Sweden)

    Terzić Sreten

    2009-01-01

    Full Text Available Several nutritive values for tubers of 114 Jerusalem artichoke (Helianthus tuberosus populations were evaluated during 2006. The used material is a part of wild sunflower species collection at the Institute of field and vegetable crops and it is situated in Rimski Šancevi, Novi Sad. The samples were analyzed as fresh tubers on 'Venema' automatic laboratory for alpha amino nitrogen, sodium and potassium content. Total sugar content was determined as the brix value on a refractometer. Total nitrogen was determined by the Kjeldahl method on dried samples. Significant variability was found for all analyzed traits. Total nitrogen varied from 0,695 to 2,179% dry weight (mean 1,23%, alpha amino nitrogen content 0,012 to 0,118% fresh weight (m. 0,07%, potassium 0,231 0,452% fresh weight (m. 0,403% and sodium 0,0003 - 0,0143% fresh weight (m. 0,007%. Total sugar content varied from 13,69 - 22,94% fresh weight (m. 19,14%. Alpha amino nitrogen is an essential nutrient for animals so that it's presence in tubers of Jerusalem artichoke as food is positive. The protein content is similar to the one in potato and as such satisfactory for nutrition. The K/Na ratio is high which is useful, because an increased content of potassium in food can positively affect the reduction of Na/K ratio and lower systolic blood pressure by a significant amount in adults with mild hypertension. Inulin makes up to 80% of the total sugar content in the tubers of Jerusalem artichoke, and as a dietary fiber and a fructose polymer it positively influences digestion and sugar blood levels. The obtained results suggest that selection of cultivars and populations with inappropriate nutritive values is possible. Further research is needed to estimate the share of genetic in total variability and to determine whether the selection for new cultivars is justified.

  8. The miRNAome of globe artichoke: conserved and novel micro RNAs and target analysis

    Directory of Open Access Journals (Sweden)

    De Paola Domenico

    2012-01-01

    Full Text Available Abstract Background Plant microRNAs (miRNAs are involved in post-transcriptional regulatory mechanisms of several processes, including the response to biotic and abiotic stress, often contributing to the adaptive response of the plant to adverse conditions. In addition to conserved miRNAs, found in a wide range of plant species a number of novel species-specific miRNAs, displaying lower levels of expression can be found. Due to low abundance, non conserved miRNAs are difficult to identify and isolate using conventional approaches. Conversely, deep-sequencing of small RNA (sRNA libraries can detect even poorly expressed miRNAs. No miRNAs from globe artichoke have been described to date. We analyzed the miRNAome from artichoke by deep sequencing four sRNA libraries obtained from NaCl stressed and control leaves and roots. Results Conserved and novel miRNAs were discovered using accepted criteria. The expression level of selected miRNAs was monitored by quantitative real-time PCR. Targets were predicted and validated for their cleavage site. A total of 122 artichoke miRNAs were identified, 98 (25 families of which were conserved with other plant species, and 24 were novel. Some miRNAs were differentially expressed according to tissue or condition, magnitude of variation after salt stress being more pronounced in roots. Target function was predicted by comparison to Arabidopsis proteins; the 43 targets (23 for novel miRNAs identified included transcription factors and other genes, most of which involved in the response to various stresses. An unusual cleaved transcript was detected for miR393 target, transport inhibitor response 1. Conclusions The miRNAome from artichoke, including novel miRNAs, was unveiled, providing useful information on the expression in different organs and conditions. New target genes were identified. We suggest that the generation of secondary short-interfering RNAs from miR393 target can be a general rule in the plant

  9. Optimization studies for the bioconversion of Jerusalem artichoke tubers to ethanol and microbial biomass

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.; Cannell, E.

    1981-01-01

    A total of 8 yeast and other microbial cultures were grown in the extract derived from the tubers of Jerusalem artichoke (Helianthus tuberosus) and screened according to the following optimization criteria: rates and yields of ethanol production, rates and yields of biomass production, and percent of original sugars utilized during fermentation. Batch growth kinetic parameters were also determined for the cultures studied. Kluyveromyces marxianus UCD (FST) 55-82 had the highest specific growth rate, 0.41/h, with a high ethanol yield, 88% of theoretical.

  10. EST2Prot: Mapping EST sequences to proteins

    Directory of Open Access Journals (Sweden)

    Lin David M

    2006-03-01

    Full Text Available Abstract Background EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. Results We describe a system (EST2Prot that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. Conclusion EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at http://biozon.org/tools/est/.

  11. Enzymatic browning and after-cooking darkening of Jerusalem artichoke tubers (Helianthus tuberosus L.)

    DEFF Research Database (Denmark)

    Bach, Vibe; Bennedbæk-Jensen, Sidsel; Clausen, Morten Rahr;

    2013-01-01

    Jerusalem artichoke tubers (Helianthus tuberosus L.) undergo enzymatic browning when peeled or cut, and turn grey after boiling, due to after-cooking darkening reactions between iron and phenolic acids. In an attempt to reveal the components responsible for these discolouration reactions, sensory...

  12. Direct lactic acid fermentation of Jerusalem artichoke tuber extract using Lactobacillus paracasei without acidic or enzymatic inulin hydrolysis.

    Science.gov (United States)

    Choi, Hwa-Young; Ryu, Hee-Kyoung; Park, Kyung-Min; Lee, Eun Gyo; Lee, Hongweon; Kim, Seon-Won; Choi, Eui-Sung

    2012-06-01

    Lactic acid fermentation of Jerusalem artichoke tuber was performed with strains of Lactobacillus paracasei without acidic or enzymatic inulin hydrolysis prior to fermentation. Some strains of L. paracasei, notably KCTC13090 and KCTC13169, could ferment hot-water extract of Jerusalem artichoke tuber more efficiently compared with other Lactobacillus spp. such as L. casei type strain KCTC3109. The L. paracasei strains could utilize almost completely the fructo-oligosaccharides present in Jerusalem artichoke. Inulin-fermenting L. paracasei strains produced c.a. six times more lactic acid compared with L. casei KCTC3109. Direct lactic fermentation of Jerusalem artichoke tuber extract at 111.6g/L of sugar content with a supplement of 5 g/L of yeast extract by L. paracasei KCTC13169 in a 5L jar fermentor produced 92.5 ce:hsp sp="0.25"/>g/L of lactic acid with 16.8 g/L fructose equivalent remained unutilized in 72 h. The conversion efficiency of inulin-type sugars to lactic acid was 98% of the theoretical yield.

  13. Establishment of fungal entomopathogens Beauveria bassiana and Bionectria ochroleuca (Ascomycota: Hypocreales) as endophytes on artichoke Cynara scolymus.

    Science.gov (United States)

    Guesmi-Jouini, J; Garrido-Jurado, I; López-Díaz, C; Ben Halima-Kamel, M; Quesada-Moraga, E

    2014-06-01

    Entomopathogenic fungi (EPF) are commonly found in diverse habitats and are known to cause mycoses in many different taxa of arthropods. Various unexpected roles have been recently reported for fungal entomopathogens, including their presence as fungal endophytes, plant disease antagonists, rhizosphere colonizers and plant growth promoting fungi. In Tunisia, a wide range of indigenous EPF isolates from different species, such as Beauveria bassiana and Bionectria ochroleuca, were found to occur in the soil, and to be pathogenic against the artichoke aphid Capitophorus elaeagni (Hemiptera: Aphididae). Since endophytic fungi are recently regarded as plant-defending mutualists and their presence in internal plant tissue has been discussed as an adaptive protection against insects, we were interested on elucidating the possible endophytic behavior of B. bassiana and B. ochroleuca on artichoke, Cynara scolymus, after foliar spraying tehcnique. The leaf spray inoculation method was effective in introducing the inoculated fungi into the plant tissues and showed, then, an endophytic activity on artichoke even 10 days later. According S-N-K test, there was significant differences between the two fungal treatments, B. ochroleuca (84% a) and B. bassiana (78% a), and controls (0% b). Likewise, the inoculated entomopathogenic fungi were also isolated from new leaves even though with significant differences respectively between controls (0% c), B. bassiana (56% b) and B. ochroleuca (78% a). These results reveals significant new data on the interaction of inoculated fungi with artichoke plant as ecological roles that can be exploited for the protection of plants.

  14. Screening of Jerusalem artichoke varieties for bio-ethanol production in Portugal

    Energy Technology Data Exchange (ETDEWEB)

    Passarinho, P.C.; Oliveira, A.C.; Rosa, M.F. [INETI, Departamento de Energias Renovaveis, Estrada do Paco do Lumiar, Ed. G, 1649-038, Lisboa (Portugal)

    2008-07-01

    The aim of this work was the evaluation of the potential of 9 Jerusalem artichoke varieties for the sustainable production of bio-ethanol in Portugal. The tubers, which are the part of the plant with higher sugar content, were harvested at different stages of development (29 to 55 weeks), and crashed for juice extraction. The two phases obtained were characterized in terms of total sugars, protein, ash and dry matter. The ethanol productivity of the different J. artichoke varieties was then evaluated fermenting juice or mixtures of juice and pulp aqueous extract with a strain of Kluyveromyces marxianus, a yeast able to hydrolyze and ferment inulin polymers. The chamical characteristic more dependable on the harvest period was the amount of total sugars in the tubers. Juices, obtained until 48 weeks development, contained 173 - 235 g/L of total sugars while juices from the last harvest presented markedly lower sugar contents, indicating crop degradation or sugar migration to the soil. Regarding the fermentative process, ethanol yields ranged from 0.3 to 0.5 g/g. The main conclusion of this work indicates C13 variety as the best. Although bearing a lower sugar concentration in tubers, the substantially higher agricultural productivities (kg/m2) after 8 months growing allowed to estimate productions higher than 10 000 L/ha.

  15. Green direct determination of mineral elements in artichokes by infrared spectroscopy and X-ray fluorescence.

    Science.gov (United States)

    Mir-Marqués, Alba; Martínez-García, Maria; Garrigues, Salvador; Cervera, M Luisa; de la Guardia, Miguel

    2016-04-01

    Near infrared (NIR) and X-ray fluorescence (XRF) spectroscopy were investigated to predict the concentration of calcium, potassium, iron, magnesium, manganese and zinc in artichoke samples. Sixty artichokes were purchased from different Spanish areas (Benicarló, Valencia and Murcia). NIR and XRF spectra, combined with partial least squares (PLS) data treatment, were used to develop chemometric models for the prediction of mineral concentration. To obtain reference data, samples were mineralised and analysed by inductively coupled plasma optical emission spectrometry (ICP-OES). Coefficients of determination obtained for the regression between predicted values and reference ones for calcium, potassium, magnesium, iron, manganese and zinc were 0.61, 0.79, 0.53, 0.77, 0.54 and 0.60 for NIR and 0.96, 0.93, 0.80, 0.79, 0.76 and 0.90 for XRF, respectively. Both assayed methodologies, offer green alternatives to classical mineral analysis, but XRF provided the best results in order to be used as a quantitative screening method.

  16. Effect of Lactobacillus paracasei Culture Filtrates and Artichoke Polyphenols on Cytokine Production by Dendritic Cells

    Science.gov (United States)

    Sisto, Angelo; Luongo, Diomira; Treppiccione, Lucia; De Bellis, Palmira; Di Venere, Donato; Lavermicocca, Paola; Rossi, Mauro

    2016-01-01

    The most recent trend in research on probiotic bacteria aims at the exploitation of bioactive bacterial compounds that are responsible for health-promoting effects and suitable for medical applications. Therefore, the main purpose of this study was to ascertain if the immunomodulatory effects of L. paracasei strains on dendritic cells (DCs) were caused by bacterial metabolites released in the culture medium. For that reason, bacterial strains were grown in two media generally used for the culture of DCs, and the effects of culture filtrates on the maturation of DCs and cytokine production were evaluated. Moreover, to reveal potential synergistic effects on the immunomodulation of DCs, an artichoke phenolic extract (APE) was added to the media before bacterial growth. The experiments pointed out an interesting anti-inflammatory activity of a culture filtrate obtained after growing a probiotic L. paracasei strain in one of the media supplemented with APE. Therefore, this culture filtrate—which combines the anti-inflammatory activity and the other well-known health-promoting properties of artichoke phenolic compounds—could represent the basis for future particular exploitations. PMID:27754398

  17. Economically Viable Components from Jerusalem Artichoke (Helianthus tuberosus L. in a Biorefinery Concept

    Directory of Open Access Journals (Sweden)

    Eva Johansson

    2015-04-01

    Full Text Available Biorefinery applications are receiving growing interest due to climatic and waste disposal issues and lack of petroleum resources. Jerusalem artichoke (Helianthus tuberosus L. is suitable for biorefinery applications due to high biomass production and limited cultivation requirements. This paper focuses on the potential of Jerusalem artichoke as a biorefinery crop and the most viable products in such a case. The carbohydrates in the tubers were found to have potential for production of platform chemicals, e.g., succinic acid. However, economic analysis showed that production of platform chemicals as a single product was too expensive to be competitive with petrochemically produced sugars. Therefore, production of several products from the same crop is a must. Additional products are protein based ones from tubers and leaves and biogas from residues, although both are of low value and amount. High bioactive activity was found in the young leaves of the crop, and the sesquiterpene lactones are of specific interest, as other compounds from this group have shown inhibitory effects on several human diseases. Thus, future focus should be on understanding the usefulness of small molecules, to develop methods for their extraction and purification and to further develop sustainable and viable methods for the production of platform chemicals.

  18. Protective Role of Ca Against NaCl Toxicity in Jerusalem Artichoke by Up-Regulation of Antioxidant Enzymes

    Institute of Scientific and Technical Information of China (English)

    XUE Yan-Feng; LIU Ling; LIU Zhao-Pu; S. K.MEHTA; ZHAO Geng-Mao

    2008-01-01

    The ameliorative effect of external Ca2+ on Jerusalem artichoke (Helianthus tuberosus L.) under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with or without 10 mol L-1 CaCl2, 150 mmol L-1 NaCl, and/or 5 mmol L-1 ethylene-bis(oxyethylenenitrilo)-tetraacetic acid (EGTA) for five days. Exposure to NaCl (150 mmol L-1) decreased growth, leaf chlorophyll content, and photosynthetic rate of Jerusalem artichoke seedlings. NaCl treatment showed 59% and 37% higher lipid peroxidation and electrolyte leakage, respectively, than the control. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were decreased by NaCl, indicating an impeded antioxidant defense mechanism of Jerusalem artichoke grown under salt stress. Addition of 10 mmol L-1 CaCl2 to the salt solutions significantly decreased the damaging effect of NaCl on growth and chlorophyll content and simultaneously restored the rate of photosynthesis almost to the level of the control. Ca2+ addition decreased the leaf malondialdehyde (MDA) content and electrolyte leakage from NaCl-treated seedlings by 47% and 24%, respectively, and significantly improved the activities of SOD, POD, and CAT in NaCl-treated plants. Addition of ECTA, a specific chelator of Ca2+, decreased the growth, chlorophyll content, and photosynthesis, and increased level of MDA and electrolyte leakage from NaCl-treated plants and from the control plants. ECTA addition to the growth medium also repressed the activities of SOD, POD, and CAT in NaCl-treated and control seedlings. External Ca2+ might protect Jerusalem artichoke against NaCl stress by up-regulating the activities of antioxidant enzymes and thereby decreasing the oxidative stress.

  19. Kinetics of ethanol production from Jerusalem artichoke juice with some Kluyveromyces species

    Energy Technology Data Exchange (ETDEWEB)

    Kuvnjak, Z.; Kosaric, N.; Hayes, R.D.

    1981-01-01

    The kinetics of ethanol production by Kluyveromyces marxianus ATCC 12708 and ATCC 10606, K. cicerisporus ATCC 22295 and K. fragilis 105 were studied using raw juice of the Jerusalem artichoke in which the carbohydrates were not hydrolyzed prior to fermentation. This juice contains enough nutrients and can serve as a complete medium without additional nutrients both for growth of the yeasts and for ethanol production. Both specific ethanol productivity and specific glucose uptake rates were the highest with K. marxianus ATCC 12708 (1.68 gg-1 h-1 and 3.78 gg-1h-1, respectively). This microorganism produced an ethanol yield of 87.5% of the theoretical value in 25 hours.

  20. Kinetics of ethanol production from Jerusalem artichoke juice with some Klugveromyces species

    Energy Technology Data Exchange (ETDEWEB)

    Duvnjak, Z.; Kosaric, N.; Hayes, R.D.

    1981-01-01

    The kinetics of ethanol produce by Kluyveromyces marxianus ATCC 12708 and ATCC 10606, K. cicerisporus ATCC 22295, and K. fragilis 105 have been studied using raw juice of the Jerusalem artichoke in which the carbohydrates were not hydrolyzed prior to fermentation. The experiments revealed that this juice contains enough nutrients and can serve as a complete medium without additional nutrients both for growth of the yeasts and for ethanol production. It was found that both specific ethanol productivity and specific uptake rates were the highest with K. marxianus ATCC 12708 (1.68 g/g/hour and 3.78 g/g/hour respectively). This microorganism produced an ethanol yield of 87.5% of the theoretical value in 25 hours. (Refs. 15).

  1. Polar constituents, protection against reactive oxygen species, and nutritional value of Chinese artichoke (Stachys affinis Bunge).

    Science.gov (United States)

    Venditti, Alessandro; Frezza, Claudio; Celona, Diana; Bianco, Armandodoriano; Serafini, Mauro; Cianfaglione, Kevin; Fiorini, Dennis; Ferraro, Stefano; Maggi, Filippo; Lizzi, Anna Rita; Celenza, Giuseppe

    2017-04-15

    In the present work, we studied the chemical composition of Chinese artichoke (S. affinis tubers) by analyzing its polar constituents and its macro- and micro- nutrients. A total of nine compounds were isolated from the tuber ethanolic extract and structurally elucidated by Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry (MS). The marker compounds identified were oligosaccharide stachyose and the organic acid, succinic acid, as well as phenylethanoid and iridoid glycosides. The macronutrient profile was dominated by carbohydrates (36.9% dw), whereas potassium (2.36%) was the most abundant micro-nutrient. The tuber ethanolic extract was able to efficiently protect human cells (Caco-2, SHSY-5Y and K562) against t-BHP-induced oxidative damage.

  2. Jerusalem artichoke as a platform for inulin, ethanol and feed production in Canada

    Energy Technology Data Exchange (ETDEWEB)

    Anyia, A.O.; Mostafa, H.; Melnichuk, R.; Slaski, J.J. [Alberta Research Council, Vegreville, AB (Canada). Bioresource Technologies Unit

    2009-07-01

    The Alberta Research Council (ARC) is developing an extraction and fermentation process for making ethanol from Jerusalem artichoke (JA). In particular, ARC has collaborated with Olds College in developing an extraction process and an engineering process for the commercial production of inulin, ethanol, polymers and animal feed from JA tubers. Fresh JA tubers contain about 20 per cent of water soluble carbohydrates, which occur primarily in the form of inulin. Several health promoting benefits are associated with intake of inulin. High volumes of dry residual aerial biomass following tuber harvest contain 40 to 50 per cent water soluble carbohydrates that are fermentable to ethanol. Some studies have shown that under optimal climatic conditions, JA can yield more ethanol per ha than sugarcane. ARC has the exclusive North American rights to several high yielding JA cultivars. Jerusalem artichoke is not a designated food crop and has a high biomass yield for soluble sugars. This perennial crop forms tubers, has a deep root system that can be adapted to marginal lands. ARC's research involves a seed to final product technology development approach that includes new variety development, agronomy and processing. ARC applied a hot water extraction technique along with a low liquid to JA stalk ratio to achieve more than 40 per cent total water soluble carbohydrates per gram of biomass that are fermentable to ethanol without the need for weak acid or enzymatic hydrolysis. A 400 hectare plantation of JA in Alberta could produce about 1,500 tonnes of inulin and 1.5 million liters of ethanol per year in a pilot scale bio-refining plant. An economic and market analysis showed that capital investments in an inulin production plant in Alberta will be a profitable venture. ARC has estimated a 5 year Internal Rate of Return (IRR) to range from 10 to 30 per cent and payback period of 4 to 5 years depending on plant location and value of by-products. tabs., figs.

  3. Jerusalem artichoke (Helianthus tuberosus L. productivity in different Italian growing areas: a modelling approach

    Directory of Open Access Journals (Sweden)

    Mario Baldini

    2011-06-01

    Full Text Available Jerusalem artichoke is considered one of the more interesting crops for inulin production. It has been used to date for the production of low calorie sweetening syrups, dietetic food enriched with fibre, medicines and cosmetics, while more recently, interesting prospects have been opening up for energy uses. The main aspects influencing its adaptability to different pedo-climatic conditions and cropping systems were analysed by implementing CSS (Cropping System Simulator, a simulation model describing crop biomass production for this specific crop. Growth analysis experimental data of plant dry matter accumulation, obtained over two years of trials (1999-2000 in different Italian growing areas (Udine, Bologna, Bari under irrigated and rain-fed conditions, were used for the parameterisation and calibration of the model. The biomass accumulation observed and simulated under rain-fed and irrigated conditions in the different growing areas is reported for the different plant organs, with good correspondence shown between simulated and measured values as reported by the statistical indices for the model calibration, particularly for biomass of tubers and leaves. The model studied, despite a simplified description of some processes, proves to represent the maximum biomass yield of Jerusalem artichoke satisfactorily, with an adequate response to the main environmental factors causing yield and biomass production variation among the years and locations. However, further model improvements are necessary in order to better represent the relationship between phenology and translocation of the assimilates between stalk and tuber during the development phases of the plant, suggesting shorter survey intervals over this growing phase.

  4. Syneresis, rheological characteristic and sensory consistency of the artichoke sauce (Cynara scolymus L.

    Directory of Open Access Journals (Sweden)

    Francisco Mora Barandiarán

    2013-09-01

    Full Text Available The effect of the concentration of three hydrocolloids: CMC, guar gum and xanthan gum on the syneresis, rheological features and sensory consistency of the artichoke sauce (Cynara scolymus L. Imperial Star variety was studied. The syneresis was determined by the water loss by centrifugation, the rheological features of the artichoke sauce was determined using a Brookfield RVDV – III model rheometer and finally, the sensory consistency was determined by measuring the degree of satisfaction with hedonic scale of nine points. A simplex lattice design blends with centroid expanded under the response surface methodology was applied to establish the effect of the concentration of hydrocolloid on syneresis, rheological features and sensory consistency. In all treatments, the apparent viscosity decreased with shear rate demonstrating a “non Newtonian” behavior of “general plastic” type with an “n” value less than 1 and an initial shear stress. The flow behavior index “n” was in the range of 0.0856 and 0.3131 (n < 1 and the consistency index “k” in the range of 84.55 y 167.80 Pa.sn , the initial shear stress was in the range of 9,10 y 13,51 Pa and consistency sensory presented score of “like” to “like slightly”. Finally, the hydrocolloid mixture was optimized over the area of feasible formulation. With the optimal mixture, corresponding to 0.28% CMC, 0.13% guar gum and 0.59% xanthan gum is expected to obtain a 0.089% of syneresis and a rating of 6 in terms of sensory consistency.

  5. Genetic mapping and identification of QTL for earliness in the globe artichoke/cultivated cardoon complex

    Directory of Open Access Journals (Sweden)

    Portis Ezio

    2012-05-01

    Full Text Available Abstract Background The Asteraceae species Cynara cardunculus (2n = 2x = 34 includes the two fully cross-compatible domesticated taxa globe artichoke (var. scolymus L. and cultivated cardoon (var. altilis DC. As both are out-pollinators and suffer from marked inbreeding depression, linkage analysis has focussed on the use of a two way pseudo-test cross approach. Results A set of 172 microsatellite (SSR loci derived from expressed sequence tag DNA sequence were integrated into the reference C. cardunculus genetic maps, based on segregation among the F1 progeny of a cross between a globe artichoke and a cultivated cardoon. The resulting maps each detected 17 major linkage groups, corresponding to the species’ haploid chromosome number. A consensus map based on 66 co-dominant shared loci (64 SSRs and two SNPs assembled 694 loci, with a mean inter-marker spacing of 2.5 cM. When the maps were used to elucidate the pattern of inheritance of head production earliness, a key commercial trait, seven regions were shown to harbour relevant quantitative trait loci (QTL. Together, these QTL accounted for up to 74% of the overall phenotypic variance. Conclusion The newly developed consensus as well as the parental genetic maps can accelerate the process of tagging and eventually isolating the genes underlying earliness in both the domesticated C. cardunculus forms. The largest single effect mapped to the same linkage group in each parental maps, and explained about one half of the phenotypic variance, thus representing a good candidate for marker assisted selection.

  6. Effects of atorvastatin and artichoke leaf tincture on oxidative stress in hypercholesterolemic rats

    Directory of Open Access Journals (Sweden)

    Crevar-Sakač Milkica

    2016-01-01

    Full Text Available Backgroung/Aim. Since combining conventional drugs with herbal medicinal products is in current research focus and possible of great interest as therapy improvement way, the aim of this study was to determine the effects of wellestablished antiatherosclerotic drug atorvastatin (CAS number 134523-00-5 and commercially available artichoke leaf tincture (ALTINC, used as combined therapy, as well as to compare effects of these two treatments separately. Methods. Experimental animals were divided into five groups: the group I (the control group of rats fed with standard diet during 11 weeks, and the remaining 4 groups of rats (II, III, IV and V fed with standard diet during the first week and then with hypercholesterolemic diet during the next 10 weeks. The group II of rats were left without treatment, while in the groups III, IV and V were rats treated per os with atorvastatin (1.15 mg/kg body wright − b.w., ALTINC (0.1 mL/kg b.w. and their combination in same doses, respectively, for the last six weeks. Results. The cholesterol rich diet led to pronounced hyperlipidemia which could not be overcame with the therapy. However, the therapy showed positive effects on abdominal aorta wall thickness and parameters of oxidative stress (malondialdehyde − MDA, proxidative-antioxidative balance − PAB and antioxidative protection (reduced glutathione − GSH, paraoxanase 1 − PON1, superoxide dismutase − SODA SH groups, especially ALTINC was successful in oxidative status improvement. Conclusion. Separate treatments comparison showed that artichoke leaf tincture is very potent antioxidant with beneficial effects in early stages of atherosclerosis. Since atorvastatin and constituents of ALTINC probably have different mechanisms of action, simultaneous use of both therapies could be beneficial but should be further investigated since our results showed that ALTINC is less effective when used in combination with atorvastatin. [Projekat Ministarstva nauke

  7. Effects of artichoke (Cynara scolymus) leaf and bloom head extracts on chemically induced DNA lesions in Drosophila melanogaster

    Science.gov (United States)

    Jacociunas, Laura Vicedo; Dihl, Rafael Rodrigues; Lehmann, Mauricio; de Barros Falcão Ferraz, Alexandre; Richter, Marc François; da Silva, Juliana; de Andrade, Heloísa Helena Rodrigues

    2014-01-01

    The genotoxicity of bloom head (BHE) and leaf (LE) extracts from artichoke (Cynara scolymus L.), and their ability to modulate the mutagenicity and recombinogenicity of two alkylating agents (ethyl methanesulfonate – EMS and mitomycin C – MMC) and the intercalating agent bleomycin (BLM), were examined using the somatic mutation and recombination test (SMART) in Drosophila melanogaster. Neither the mutagenicity nor the recombinogenicity of BLM or MMC was modified by co- or post-treatment with BHE or LE. In contrast, co-treatment with BHE significantly enhanced the EMS-induced genotoxicity involving mutagenic and/or recombinant events. Co-treatment with LE did not alter the genotoxicity of EMS whereas post-treatment with the highest dose of LE significantly increased this genotoxicity. This enhancement included a synergistic increase restricted to somatic recombination. These results show that artichoke extracts promote homologous recombination in proliferative cells of D. melanogaster. PMID:24688296

  8. Influence of cooking conditions on organoleptic and health-related properties of artichokes, green beans, broccoli and carrots.

    Science.gov (United States)

    Guillén, Sofía; Mir-Bel, Jorge; Oria, Rosa; Salvador, María L

    2017-02-15

    Colour, pigments, total phenolic content and antioxidant activity were investigated in artichokes, green beans, broccoli and carrots cooked under different conditions. Domestic induction hobs with temperature control were used to evaluate the effect of boiling, sous-vide cooking and water immersion cooking at temperatures below 100°C on the properties of each vegetable. Sous-vide cooking preserved chlorophyll, carotenoids, phenolic content and antioxidant activity to a greater extent than boiling for all of the vegetables tested and retained colour better, as determined by a(∗). A reduction of only 10-15°C in the cooking temperature was enough to improve the properties of the samples cooked by water immersion, except for green beans. Artichokes and carrots suffered pronounced losses of antioxidant activity during boiling (17.0 and 9.2% retention, respectively), but the stability of this parameter significantly increased with sous-vide cooking (84.9 and 55.3% retention, respectively).

  9. Cellulosic bioethanol production from Jerusalem artichoke (Helianthus tuberosus L.) using hydrogen peroxide-acetic acid (HPAC) pretreatment.

    Science.gov (United States)

    Song, Younho; Wi, Seung Gon; Kim, Ho Myeong; Bae, Hyeun-Jong

    2016-08-01

    Jerusalem artichoke (JA) is recognized as a suitable candidate biomass crop for bioethanol production because it has a rapid growth rate and high biomass productivity. In this study, hydrogen peroxide-acetic acid (HPAC) pretreatment was used to enhance the enzymatic hydrolysis and to effectively remove the lignin of JA. With optimized enzyme doses, synergy was observed from the combination of three different enzymes (RUT-C30, pectinase, and xylanase) which provided a conversion rate was approximately 30% higher than the rate with from treatment with RUT-C30 alone. Fermentation of the JA hydrolyzates by Saccharomyces cerevisiae produced a fermentation yield of approximately 84%. Therefore, Jerusalem artichoke has potential as a bioenergy crop for bioethanol production.

  10. The content of protein and of amino acids in Jerusalem artichoke tubers (Helianthus tuberosus L. of red variety Rote Zonenkugel

    Directory of Open Access Journals (Sweden)

    Ewa Cieślik

    2011-12-01

    Full Text Available   Introduction. Jerusalem artichoke (Helianthus tuberosus L. is grown primarily for its edible tubers, which were first cultivated by native Americans before the arrival of the Europeans. Unlike most tubers, but in common with other members of the Asteraceae, the tubers store fructans instead of starch. Fructans are non-digestible carbohydrates considered functional food ingredients because they affect body processes in ways that result in better health and in many diseases prevention. However, the Jerusalem artichoke deserves attention not only because of the content of fructans, recent studies also indicate a high protein content, including essential amino acids. Material and methods. The aim of the work was to establish the content of protein and amino acids in Jerusalem artichoke tubers (Helianthus tuberosus L. of red variety – Rote Zonenkugel. The content of protein was estimated by Dumas method. The amino acids composition was analysed with ion-change chromatography with postcolumn derivatisation and detection of ninhydryn reaction with automatic amino acids analyser. Results. The assessed liophylisate was characterised by high protein content (6.36% in comparison to chicory (which is the main industrial source of fructans and to commonly consumed potatoes. There was shown a few times higher content of essential amino acids (also of methionine in comparison to chicory and potato. The examined essential amino acids were present in very advantagenous proportions. Conclusions. In Jerusalem artichoke tubers of Rote Zonenkugel variety of the high content of protein was established in comparison to other plant sources. The high content was found of amino acids with special stress on essential amino acids (esp. sulphur ones.  

  11. Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor.

    Science.gov (United States)

    Huang, Jin; Cai, Jin; Wang, Jin; Zhu, Xiangcheng; Huang, Lei; Yang, Shang-Tian; Xu, Zhinan

    2011-02-01

    Butyric acid is an important specialty chemical with wide industrial applications. The feasible large-scale fermentation for the economical production of butyric acid requires low-cost substrate and efficient process. In the present study, butyric acid production by immobilized Clostridium tyrobutyricum was successfully performed in a fibrous-bed bioreactor using Jerusalem artichoke as the substrate. Repeated-batch fermentation was carried out to produce butyric acid with a high butyrate yield (0.44 g/g), high productivity (2.75 g/L/h) and a butyrate concentration of 27.5 g/L. Furthermore, fed-batch fermentation using sulfuric acid pretreated Jerusalem artichoke hydrolysate resulted in a high butyric acid concentration of 60.4 g/L, with the yield of 0.38 g/g and the selectivity of ∼ 85.1 (85.1g butyric acid/g acetic acid). Thus, the production of butyric acid from Jerusalem artichoke on a commercial scale could be achieved based on the system developed in this work.

  12. Conte nt of nutritive components, dietary fi bre and energy value of artichoke depending on the variety

    Directory of Open Access Journals (Sweden)

    Iwona Mentel

    2012-06-01

    Full Text Available Background. The artichoke (Cynara scolymus L. is a perennial plant belonging to Asteraceae family. It is less popular vegetable in Poland but very valuable from nutritive and medicinal points of view plant. Material and methods. The experiments were conducted in 2008 and 2009 on fi ve artichoke varieties: ‘Deutscher Hybrid’, ‘Emerald F1’, Green Globe’, ‘Gros Camus de Bretague’ and ‘Kerlouan’, grew in Poland, France and Austria. The experimental material was assessed for: dry mass, protein, ash, vitamin C and dietary fi ber contents and some mineral components (calcium, magnesium, phosphorus, potassium, sodium, iron, zinc levels. Also the energy value and total carbohydrates content were calculated in the vegetable. Results. Among assessed varieties, the highest contents of such components as: dry mass (total solids, carbohydrates, ash, vitamin C, sodium, potassium, magnesium, iron were found in ‘Gros Camus de Bretague’. The hearts of that artichoke had also higher energy value than others. Whereas the lowest ash, phosphorus, sodium, potassium and magnesium were noticed in ‘Kerlouan’ variety. But the highest values of dietary fi ber was observed in that variety. Conclusions. Basing on performed assessments the differences in nutritive components and dietary fi ber, between analysed vegetable varieties were found.

  13. Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid

    Directory of Open Access Journals (Sweden)

    Tanupat Mornkham

    2013-04-01

    Full Text Available Jerusalem artichoke (Helianthus tuberosus L. is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis challenging. This study was aimed to improve existing methods for extracting total RNA from Jerusalem artichoke dry seeds and to assess the applicability of the improved method in other plant species. Five RNA extraction methods were evaluated on Jerusalem artichoke seeds and two were modified. One modified method with the significant improvement was applied to assay seeds of diverse Jerusalem artichoke accessions, sunflower, rice, maize, peanut and marigold. The effectiveness of the improved method to extract total RNA from seeds was assessed using qPCR analysis of four selected genes. The improved method of Ma and Yang (2011 yielded a maximum RNA solubility and removed most interfering substances. The improved protocol generated 29 to 41 µg RNA/30 mg fresh weight. An A260/A280 ratio of 1.79 to 2.22 showed their RNA purity. Extracted RNA was effective for downstream applications such as first-stranded cDNA synthesis, cDNA cloning and qPCR. The improved method was also effective to extract total RNA from seeds of sunflower, rice, maize and peanut that are rich in polyphenols, lipids and polysaccharides.

  14. galaxieEST: addressing EST identity through automated phylogenetic analysis

    Directory of Open Access Journals (Sweden)

    Larsson Karl-Henrik

    2004-07-01

    Full Text Available Abstract Background Research involving expressed sequence tags (ESTs is intricately coupled to the existence of large, well-annotated sequence repositories. Comparatively complete and satisfactory annotated public sequence libraries are, however, available only for a limited range of organisms, rendering the absence of sequences and gene structure information a tangible problem for those working with taxa lacking an EST or genome sequencing project. Paralogous genes belonging to the same gene family but distinguished by derived characteristics are particularly prone to misidentification and erroneous annotation; high but incomplete levels of sequence similarity are typically difficult to interpret and have formed the basis of many unsubstantiated assumptions of orthology. In these cases, a phylogenetic study of the query sequence together with the most similar sequences in the database may be of great value to the identification process. In order to facilitate this laborious procedure, a project to employ automated phylogenetic analysis in the identification of ESTs was initiated. Results galaxieEST is an open source Perl-CGI script package designed to complement traditional similarity-based identification of EST sequences through employment of automated phylogenetic analysis. It uses a series of BLAST runs as a sieve to retrieve nucleotide and protein sequences for inclusion in neighbour joining and parsimony analyses; the output includes the BLAST output, the results of the phylogenetic analyses, and the corresponding multiple alignments. galaxieEST is available as an on-line web service for identification of fungal ESTs and for download / local installation for use with any organism group at http://galaxie.cgb.ki.se/galaxieEST.html. Conclusions By addressing sequence relatedness in addition to similarity, galaxieEST provides an integrative view on EST origin and identity, which may prove particularly useful in cases where similarity searches

  15. Germinação in vitro de sementes de alcachofra In vitro artichoke seed germination

    Directory of Open Access Journals (Sweden)

    Cassieli F de Moraes

    2010-03-01

    Full Text Available A baixa taxa de multiplicação e alta de contaminação dos explantes são algumas das dificuldades na micropropagação da alcachofra. A germinação de sementes in vitro pode ser uma alternativa de obtenção de explantes sadios para estabelecimento de futuros cultivos in vitro. O trabalho desenvolvido no Laboratório de Biotecnologia Vegetal da UPF-FAMV teve por objetivo avaliar a germinação in vitro de sementes de alcachofra cv. Nobre, em três experimentos, testando concentrações de cloro ativo na assepsia das sementes; tratamentos do tegumento (mantido intacto, com cortes laterais ou eliminação; condições de luminosidade (claro ou escuro; e dois meios de cultura [meio MS, com concentração de sais reduzida à metade (M1 e meio MS completo(M2]. Em ambos foram adicionados 30 g L-1 de sacarose e 7 g L-1 de ágar, sendo o pH ajustado para 5,6 ± 0,1 com NaOH. Os cultivos foram realizados em câmara de crescimento. A obtenção de plântulas sadias de alcachofra em curto espaço de tempo (sete dias, para utilização como fonte de explantes é viável a partir da germinação in vitro de sementes sem o tegumento (77,5% de germinação, utilizando os meios de cultura M1 ou M2 e câmara de crescimento desprovida de luz. Nestas condições, a assepsia das sementes pode ser realizada com álcool 70% por 30 min e posterior imersão em solução contendo 2% de cloro ativo por dez minutos, antes da remoção do tegumento.Low multiplication rates and high contamination in the explants are some of the difficulties in artichoke micropropagation. In vitro seed germination may be an alternative to obtain healthy explants for use in future in vitro cultivation. This project developed at the laboratory of Universidade de Passo Fundo was established to evaluate cv. 'Nobre' artichoke seeds in vitro germination. In three experiments, active chloride concentrations on seed aseptic technique; tegument treatment (kept intact, with side cuts and

  16. Research Progress of Exploitation and Utilization of Jerusalem artichoke Resources%菊芋资源开发及利用研究进展

    Institute of Scientific and Technical Information of China (English)

    郭洪涛; 郭衍银

    2011-01-01

    本文在阐述菊芋价值、菊糖特性的基础上,对菊糖提取方法、菊芋制品研究现状进行了综述,同时就菊芋资源开发和利用中存在问题和发展方向提出了几点看法.%On the basis of elaborating the value of Jerusalem artichoke and the properties of inulin, the research situation of inulin extraction methods and Jerusalem artichoke products was summarized in this paper. Meanwhile, some suggestions were put forward for the present problems and developing direction in exploitation and utilization of Jerusalem artichoke.

  17. Relational databases

    CERN Document Server

    Bell, D A

    1986-01-01

    Relational Databases explores the major advances in relational databases and provides a balanced analysis of the state of the art in relational databases. Topics covered include capture and analysis of data placement requirements; distributed relational database systems; data dependency manipulation in database schemata; and relational database support for computer graphics and computer aided design. This book is divided into three sections and begins with an overview of the theory and practice of distributed systems, using the example of INGRES from Relational Technology as illustration. The

  18. CitEST libraries

    Directory of Open Access Journals (Sweden)

    Maria Luísa P. Natividade Targon

    2007-01-01

    Full Text Available In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia. In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5’ end, generating 242,790 valid reads of citrus. A total of 9,504 sequences were produced in the shotgun library and the valid reads were assembled using CAP3. In this procedure, we obtained 1,131 contigs and 4,083 singletons. A total of 19,200 cDNA clones from P. parasitica were sequenced, resulting in 16,400 valid reads. The number of ESTs generated in this project is, to our knowledge, the largest citrus sequence database in the world.

  19. Biofuel Database

    Science.gov (United States)

    Biofuel Database (Web, free access)   This database brings together structural, biological, and thermodynamic data for enzymes that are either in current use or are being considered for use in the production of biofuels.

  20. Community Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This excel spreadsheet is the result of merging at the port level of several of the in-house fisheries databases in combination with other demographic databases such...

  1. Preparation of Inulin Powder from Jerusalem Artichoke (Helianthus tuberosus L.) Tuber.

    Science.gov (United States)

    Srinameb, Bang-orn; Nuchadomrong, Suporn; Jogloy, Sanun; Patanothai, Aran; Srijaranai, Supalax

    2015-06-01

    The complete procedure for the production of inulin powder from Jerusalem artichoke tubers (JAT) was investigated. The procedure consists of isolation of inulin from JAT, elimination of color from the inulin extract and solidification. Washed tubers were first sliced, dried in a 60 °C oven for 10 h and then milled and sieved into a powder. Inulin was isolated from the JAT powder by hot water extraction using an accelerated solvent extractor (ASE). The effects of temperature and time for the extraction were investigated. The highest extraction efficiency was obtained at the extraction temperature of 80 °C for 20 min. The color of the extract was eliminated using ion exchange process with diethylaminoethyl cellulose as the sorbent. The inulin powder was subsequently obtained by freeze drying. Inulin content and inulin profiles were monitored to evaluate the efficiencies of the complete procedure. The inulin content was indirectly determined by spectrophotometry from free and total fructose measurements using potassium iodide. The inulin profile was monitored using high performance anion exchange chromatography equipped with integrated pulse amperometric detection (HPAEC-PAD). The proposed method provided the inulin production yield of 92.5%. The present procedure is fast, simple and effective for production of inulin powder from JAT. In addition, infrared spectra and some physico-chemical properties of the obtained inulin powder were determined and compared with the standard inulin.

  2. Effect of Seawater Stress on Physiological and Biochemical Responses of Five Jerusalem Artichoke Ecotypes

    Institute of Scientific and Technical Information of China (English)

    LONG Xiao-Hua; CHI Jin-He; LIU Ling; LI Qing; LIU Zhao-Pu

    2009-01-01

    Three treatments consisting of 0%,15%,and 30% seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke (Helianthus tuberosus) regarding their responses to seawater stress under a hydroponic culture system.Analyses were done 2,4,and 6 days after treatments.The 15% and 30% seawater treatments reduced the growth rates of roots and shoots of H.tuberosus populations.The activities of superoxide dismutase,peroxidase,and catalase majored in the leaves were stimulated under the seawater stress.The electrolyte leakage and malondialdehyde contents of the leaves were also stimulated owing to seawater stress.The contents of proline and solublesugars in the leaves increased significantly with increasing seawater concentrations.The concentrations of Na+,K+,and Cl-in the aerial parts and roots increased with an increase in the seawater concentration throughout the experimental period.There were ccotypic differences among the five populations of H.tuberosus as evidenced by the analyses of the above items in both aerial parts and roots under seawater treatment.The magnitude of the ecotypic variance components indicated that a substantial proportion of the total variation for these physiological and biochemical responses were owing to ecotype,indicating the possibility of improvement through hybridization and selection.

  3. Potential environmental impact of effluents from the artichoke (Cynara scolymus L.) byproduct ensiling process using additives.

    Science.gov (United States)

    Megías, M D; Martínez-Teruel, A; Hérnandez, M R

    1999-06-01

    Three treatments have been tested on canned artichoke byproduct after 50 days of ensilage: formic acid at 20% in doses of 2 mL. kg(-)(1) (FA), cane sugar molasses at 50 g.kg(-)(1) (M), and sodium chloride at 30 g.kg(-)(1) (SC). A fourth batch acted as a control group (C). The nutritive value, fermentation characteristics, environmental pollution effect, and total volume of effluents released have been studied. The highest nutritive value recorded was with SC silage. The use of the additives did not significantly improve the fermentation stability of the silage, but the total production of effluents in each treatment-52.7 (FA), 46.9 (M), and 55.2 (SC)-was significantly lower (P < 0.01) than that of the control group (70.1 L.Tm(-)(1)). The chemical oxygen demand (COD), 117300 mg of O(2).L(-)(1), and the conductivity, 46.4 microOmega(-)(1). cm(-)(1), were significantly higher (P < 0.01) in M and SC, respectively, than in the other group.

  4. Ethanol inhibition kinetics of Kluyveromyces marxianus grown on Jerusalem artichoke juice

    Energy Technology Data Exchange (ETDEWEB)

    Bajpai, P.; Margaritis, A.

    1982-12-01

    The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (weight/volume) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentrations ranging from 0 to 80 g/liter in the liquid medium were used to study the inhibitory effect of ethanol on the following parameters: maximum specific growth rate (mu max), cell and ethanol yields, and sugar utilization. It was found that as the initial ethanol concentration increased from 0 to 80 g/liter, and maximum specific growth rate of K. marxianus cells decreased from 0.42 to 0.09/hour, whereas the ethanol and cell yields and sugar utilization remained almost constant. A simple kinetic model was used to correlate the mu max results and the rates of cell and ethanol production, and the appropriate constants were evaluated. (Refs. 22).

  5. Continuous ethanol production from Jerusalem artichoke tubers. I. Use of free cells of Kluyveromyces marxianus

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.

    1982-07-01

    The continuous fermentation of Jerusalem artichoke juice to ethanol by free cells of Kluyveromyces marxianus UCD (FST) 55-82 has been studied in a continuous-stirred tank bioreactor at 35 degrees C and pH 4.6. A maximum ethanol yield of 90% of the theoretical was obtained at a dilution rate of 0.05/h. About 95% of the sugars were utilized at dilution rates lower than 0.15/h. Volumetric ethanol productivity and volumetric biomass productivity reached maximum values of 7 g EtOH/L/h and 0.6 g dry wt/L/h, respectively, at a dilution rate of 0.2h. The maintenance energy coefficient for Kluyveromyces marxianus culture was found to be 0.46 g sugar/g biomass/h. Oscillatory behavior was observed following a change in dilution rate from a previous steady state and from batch to continuous culture. Values of specific ethanol production rate and specific sugar uptake were found to increase almost linearly with the increase of the dilution rate. The maximum specific ethanol production rate and maximum specific sugar uptake rate were found to be 2.6 g ethanol/g cell/h and 7.9 sugars/g cell/h, respectively. Washout occurred at a dilution rate of 0.41/h. (Refs. 21).

  6. Continuous ethanol production from Jerusalem artichokes stalks using immobilized cells of Kluyveromyces marxianus

    Energy Technology Data Exchange (ETDEWEB)

    Bajpai, P.; Margaritis, A.

    1986-01-01

    Continuous production of ethanol from the extract of Jerusalem artichoke stalks was investigated in a packed bed bioreactor using Kluyveromyces marxianus cells immobilized in calcium alginate gel beds. Maximum conversion of the sugars to ethanol was achieved with a yield of about 98% of the theoretical. Volumetric ethanol productivities of 102 grams of ethanol per litre per hour and 92 grams ethanol per liter per hour were obtained at 87% and 90% conversion respectively for an inlet substrate concentration of 100 gram sugars per liter. The maximum specific ethanol production rate and maximum specific total sugar uptake rate of the immobilized cells were found to be 0.96 gram ethanol per gram immobilized cells per hour and 2.06 gram sugars per gram immobilized cells per hour respectively. The immobilized cell bioreactor was run continuously at a dilution rate of 2.12 per hour for 30 days which resulted in a loss of 30% of the original activity. The half life of the bioreactor was estimated to be about 56 days.

  7. Continuous ethanol production from Jerusalem artichoke tubers. II. Use of immobilized cells of Kluyveromyces marxianus

    Energy Technology Data Exchange (ETDEWEB)

    Margaritis, A.; Bajpai, P.

    1982-07-01

    Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/L/h were obtained at 80 and 92% sugar utilization, respectively. The maximum volumetric ethanol productivity of the immobilized cell bioreactor system was found to be 15 times higher than that of an ordinary continuous-stirred-tank (CST) bioreactor using free cells of Kluyveromyces marxianus. The immobilized cell bioreactor system was operated continuously at a constant dilution rate of 0.66/h for 12 days resulting in only an 8% loss of the original immobilized cell activity, which corresponds to an estimated half-life of ca. 72 days. The maximum specific ethanol productivity and maximum specific sugar uptake rate of the immobilized cells were found to be 0.55 g ethanol/g biomass/h and 1.21 g sugars/g biomass/h, respectively. (Refs. 27).

  8. Conversion from long-term cultivated wheat field to Jerusalem artichoke plantation changed soil fungal communities

    Science.gov (United States)

    Zhou, Xingang; Zhang, Jianhui; Gao, Danmei; Gao, Huan; Guo, Meiyu; Li, Li; Zhao, Mengliang; Wu, Fengzhi

    2017-01-01

    Understanding soil microbial communities in agroecosystems has the potential to contribute to the improvement of agricultural productivity and sustainability. Effects of conversion from long-term wheat plantation to Jerusalem artichoke (JA) plantation on soil fungal communities were determined by amplicon sequencing of total fungal ITS regions. Quantitative PCR and PCR-denaturing gradient gel electrophoresis were also used to analyze total fungal and Trichoderma spp. ITS regions and Fusarium spp. Ef1α genes. Results showed that soil organic carbon was higher in the first cropping of JA and Olsen P was lower in the third cropping of JA. Plantation conversion changed soil total fungal and Fusarium but not Trichoderma spp. community structures and compositions. The third cropping of JA had the lowest total fungal community diversity and Fusarium spp. community abundance, but had the highest total fungal and Trichoderma spp. community abundances. The relative abundances of potential fungal pathogens of wheat were higher in the wheat field. Fungal taxa with plant growth promoting, plant pathogen or insect antagonistic potentials were enriched in the first and second cropping of JA. Overall, short-term conversion from wheat to JA plantation changed soil fungal communities, which is related to changes in soil organic carbon and Olsen P contents.

  9. Database Administrator

    Science.gov (United States)

    Moore, Pam

    2010-01-01

    The Internet and electronic commerce (e-commerce) generate lots of data. Data must be stored, organized, and managed. Database administrators, or DBAs, work with database software to find ways to do this. They identify user needs, set up computer databases, and test systems. They ensure that systems perform as they should and add people to the…

  10. Production of ethanol at high temperatures in the fermentation of Jerusalem artichoke juice and a simple medium by Kluyveromyces marxianus

    Energy Technology Data Exchange (ETDEWEB)

    Rosa, M.F.; Correia, I.S.; Novais, J.M.

    1987-01-01

    Temperatures as high as 36 degrees C and 40 degrees C did not negatively affect the ethanol productivity of Jerusalem artichoke (J.a.) juice batch fermentation and the final concentrations of ethanol were close to those produced at lower temperatures. At higher process temperatures (36-40 degrees C), ethanol toxicity in Kluyveromyces marxianus was less important during the fermentation of J.a. juice as compared with a simple medium. In simple medium, the heat-sticking of fermentation was observed and the percentage of unfermented sugars steeply increased from 28 degrees C up to 40 degrees C. (Refs. 13).

  11. Artichoke compound cynarin differentially affects the survival, growth and stress response of normal, immortalized and cancerous human cells

    DEFF Research Database (Denmark)

    Gezer, Ceren; Yücecan, Sevinç; Rattan, Suresh Inder Singh

    2015-01-01

    Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. Potential health-beneficial effects of CYN include as being choloretic-cholesterol lowering, hepatoprotective, anti-atherosclerotic, and antioxidative. We have tested the effects of various doses...... of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers haemoxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC) and cervical cancer cells, HeLa. Effects of CYN...

  12. Variation of inulin content, inulin yield and water use efficiency for inulin yield in Jerusalem artichoke genotypes under different water regimes

    Science.gov (United States)

    The information on genotypic variation for inulin content, inulin yield and water use efficiency of inulin yield (WUEi) in response to drought is limited. This study was to investigate the genetic variability in inulin content, inulin yield and WUEi of Jerusalem artichoke (Helianthus tuberosus L.) ...

  13. Free-range pigs foraging on Jerusalem artichokes (Helianthus tuberosus L.) – Effect of feeding strategy on growth, feed conversion and animal behaviour

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Horsted, Klaus; Hermansen, John Erik

    2013-01-01

    The nutritional contributions from free-range foraging, growth, feed conversion and behaviour were investigated in 36 growing pigs foraging on Jerusalem artichokes (JA) and fed concentrates restrictedly (30% of energy recommendations) or ad libitum. Compared to the ad libitum fed pigs, the pigs f...

  14. Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress

    NARCIS (Netherlands)

    Moglia, A.; Comino, C.; Portis, E.; Acquadro, A.; Vos, de C.H.; Beekwilder, M.J.; Lanteri, S.

    2009-01-01

    Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe arti

  15. In vitro callus-induction in globe artichoke (Cynara cardunculus L. var. scolymus) as a system for the production of caffeoylquinic acids

    NARCIS (Netherlands)

    Menin, B.; Moglia, A.; Comino, C.; Hakkert, J.C.; Lanteri, S.; Beekwilder, M.J.

    2013-01-01

    Globe artichoke (Cynara cardunculus L. var. scolymus) provides a rich dietary source of bio-active compounds derived from phenylpropanoid metabolism, notably caffeoylquinic acids (CQAs) and flavonoids. Micropropagation techniques have been established for this species, but in vitro cultures have not

  16. First detailed karyo-morphological analysis and molecular cytological study of leafy cardoon and globe artichoke, two multi-use Asteraceae crops

    Directory of Open Access Journals (Sweden)

    Debora Giorgi

    2016-09-01

    Full Text Available Traditionally globe artichoke and leafy cardoon have been cultivated for use as vegetables but these crops are now finding multiple new roles in applications ranging from paper production to cheese preparation and biofuel use, with interest in their functional food potential. So far, their chromosome complements have been poorly investigated and a well-defined karyotype was not available. In this paper, a detailed karyo-morphological analysis and molecular cytogenetic studies were conducted on globe artichoke (Cynara cardunculus Linnaeus, 1753 var. scolymus Fiori, 1904 and leafy cardoon (C. cardunculus Linneaus, 1753 var. altilis De Candolle, 1838. Fluorescent In Situ Hybridization In Suspension (FISHIS was applied to nuclei suspensions as a fast method for screening of labelling probes, before metaphase spread hybridization. Classic Fluorescent In Situ Hybridization (FISH on slide, using repetitive telomeric and ribosomal sequences and Simple Sequence Repeats (SSRs oligonucleotide as probes, identified homologous chromosome relationships and allowed development of molecular karyotypes for both varieties. The close phylogenetic relationship between globe artichoke and cardoon was supported by the very similar karyotypes but clear chromosomal structural variation was detected. In the light of the recent release of the globe artichoke genome sequencing, these results are relevant for future anchoring of the pseudomolecule sequence assemblies to specific chromosomes. In addition, the DNA content of the two crops has been determined by flow cytometry and a fast method for standard FISH on slide and methodological improvements for nuclei isolation are described.

  17. First detailed karyo-morphological analysis and molecular cytological study of leafy cardoon and globe artichoke, two multi-use Asteraceae crops

    Science.gov (United States)

    Giorgi, Debora; Pandozy, Gianmarco; Farina, Anna; Grosso, Valentina; Lucretti, Sergio; Gennaro, Andrea; Crinò, Paola; Saccardo, Francesco

    2016-01-01

    Abstract Traditionally globe artichoke and leafy cardoon have been cultivated for use as vegetables but these crops are now finding multiple new roles in applications ranging from paper production to cheese preparation and biofuel use, with interest in their functional food potential. So far, their chromosome complements have been poorly investigated and a well-defined karyotype was not available. In this paper, a detailed karyo-morphological analysis and molecular cytogenetic studies were conducted on globe artichoke (Cynara cardunculus Linnaeus, 1753 var. scolymus Fiori, 1904) and leafy cardoon (Cynara cardunculus Linneaus, 1753 var. altilis De Candolle, 1838). Fluorescent In Situ Hybridization In Suspension (FISHIS) was applied to nuclei suspensions as a fast method for screening of labelling probes, before metaphase spread hybridization. Classic Fluorescent In Situ Hybridization (FISH) on slide, using repetitive telomeric and ribosomal sequences and Simple Sequence Repeats (SSRs) oligonucleotide as probes, identified homologous chromosome relationships and allowed development of molecular karyotypes for both varieties. The close phylogenetic relationship between globe artichoke and cardoon was supported by the very similar karyotypes but clear chromosomal structural variation was detected. In the light of the recent release of the globe artichoke genome sequencing, these results are relevant for future anchoring of the pseudomolecule sequence assemblies to specific chromosomes. In addition, the DNA content of the two crops has been determined by flow cytometry and a fast method for standard FISH on slide and methodological improvements for nuclei isolation are described. PMID:27830052

  18. EST-PAC a web package for EST annotation and protein sequence prediction

    Directory of Open Access Journals (Sweden)

    Strahm Yvan

    2006-10-01

    Full Text Available Abstract With the decreasing cost of DNA sequencing technology and the vast diversity of biological resources, researchers increasingly face the basic challenge of annotating a larger number of expressed sequences tags (EST from a variety of species. This typically consists of a series of repetitive tasks, which should be automated and easy to use. The results of these annotation tasks need to be stored and organized in a consistent way. All these operations should be self-installing, platform independent, easy to customize and amenable to using distributed bioinformatics resources available on the Internet. In order to address these issues, we present EST-PAC a web oriented multi-platform software package for expressed sequences tag (EST annotation. EST-PAC provides a solution for the administration of EST and protein sequence annotations accessible through a web interface. Three aspects of EST annotation are automated: 1 searching local or remote biological databases for sequence similarities using Blast services, 2 predicting protein coding sequence from EST data and, 3 annotating predicted protein sequences with functional domain predictions. In practice, EST-PAC integrates the BLASTALL suite, EST-Scan2 and HMMER in a relational database system accessible through a simple web interface. EST-PAC also takes advantage of the relational database to allow consistent storage, powerful queries of results and, management of the annotation process. The system allows users to customize annotation strategies and provides an open-source data-management environment for research and education in bioinformatics.

  19. Development of an automated method for Folin-Ciocalteu total phenolic assay in artichoke extracts.

    Science.gov (United States)

    Yoo, Kil Sun; Lee, Eun Jin; Leskovar, Daniel; Patil, Bhimanagouda S

    2012-12-01

    We developed a system to run the Folin-Ciocalteu (F-C) total phenolic assay, in artichoke extract samples, which is fully automatic, consistent, and fast. The system uses 2 high performance liquid chromatography (HPLC) pumps, an autosampler, a column heater, a UV/Vis detector, and a data collection system. To test the system, a pump delivered 10-fold diluted F-C reagent solution at a rate of 0.7 mL/min, and 0.4 g/mL sodium carbonate at a rate of 2.1 mL/min. The autosampler injected 10 μL per 1.2 min, which was mixed with the F-C reagent and heated to 65 °C while it passed through the column heater. The heated reactant was mixed with sodium carbonate and color intensity was measured by the detector at 600 nm. The data collection system recorded the color intensity, and peak area of each sample was calculated as the concentration of the total phenolic content, expressed in μg/mL as either chlorogenic acid or gallic acid. This new method had superb repeatability (0.7% CV) and a high correlation with both the manual method (r(2) = 0.93) and the HPLC method (r(2) = 0.78). Ascorbic acid and quercetin showed variable antioxidant activity, but sugars did not. This method can be efficiently applied to research that needs to test many numbers of antioxidant capacity samples with speed and accuracy.

  20. Influence of nitrogen fertilization on diazotrophic communities in the rhizosphere of the Jerusalem artichoke (Helianthus tuberosus L.).

    Science.gov (United States)

    Meng, Xianfa; Wang, Lin; Long, Xiaohua; Liu, Zhaopu; Zhang, Zhenhua; Zed, Rengel

    2012-06-01

    Diazotrophs in the soil may be influenced by plant factors as well as nitrogen (N) fertilization. In this study, we investigated potential diazotrophic communities in the rhizosphere of the Jerusalem artichoke (Helianthus tuberosus L.) supplied with differing amounts of N. The community structure of N(2)-fixing bacteria was profiled using the length heterogeneity polymerase chain reaction (LH-PCR) and terminal restriction fragment length polymorphism (T-RFLP) based on a variation in the nifH gene. Higher numbers of diazotrophs were detected by T-RFLP compared to LH-PCR. The lowest number of N(2)-fixing bacteria was observed in the rhizosphere soil with high N fertilization. T-RFLP was a better method than LH-PCR for profiling microbial diversity of diazotrophs using multidimensional scaling (MDS) and analysis of similarity (ANOSIM) of fingerprints as well as diversity measures. The supply of N fertilizer appeared to negatively influence the abundance of diazotrophs in the rhizophere of the Jerusalem artichoke.

  1. Effect of cultivar x ozone treatment interaction on the total polyphenols content and antioxidant activity of globe artichoke

    Directory of Open Access Journals (Sweden)

    Sara Lombardo

    2015-06-01

    Full Text Available Two globe artichoke cultivars (Violet de Provence and Apollo were harvested at an experimental field in Sicily, immediately washed with ozonised water and stored in: i normal atmosphere; ii ozone-enriched atmosphere for 3 days and for the last 4 days in normal atmosphere; iii ozone-enriched atmosphere for 7 days. A control (samples unwashed and stored at room temperature was also investigated. The effect of cultivar x ozone treatment interaction on water content losses, total polyphenols content and antioxidant activity was evaluated after 0, 3 and 7 days of storage. Washing with ozonised water and storage under O3- enriched atmosphere allowed higher water retention compared with the control, especially for Violet de Provence. After 3 days of storage in ozone-enriched atmosphere, on average of cultivars, the total polyphenols content and antioxidant activity increased by 11.7% and 5.5%, respectively. By contrast, after further 4 days of storage in ozonised atmosphere, Apollo and Violet de Provence displayed a significant reduction in their level of total polyphenols and aantioxidant activity. The exposure of globe artichoke heads to an ozone-enriched atmosphere should not exceed 3 days aimed at preserving their high nutritional value, with special emphasis on total polyphenols content.

  2. Comparison of some biochemical properties of artichoke polyphenol oxidase entrapped in alginate-carrageenan and alginate gels.

    Science.gov (United States)

    Yagar, Hulya; Kocaturk, Selin

    2014-08-01

    Polyphenol oxidase (PPO, EC.1.14.18.1) isolated from artichoke (Cynara scolymus) was entrapped within alginate and alginate+ carrageenan beads, and the catecholase and cresolase activities of both entrapped enzymes were determined. Some properties of these immobilized enzymes such as optimum pH and temperature, kinetic parameters (Km and Vmax), thermal, and storage stability were determined and compared to each other. The highest catecholase activity was observed in alginate gel (370 U/g bead) while the highest cresolase activity was in alginate+ carrageenan gel (90 U/g bead). For catecholase and cresolase activities, optimum pHs of alginate and alginate+ carrageenan beads were determined to be 7.0 and 4.0, respectively. Optimum temperatures for catecholase activity were determined to be 40°C for both entrapped enzymes. These values for cresolase activity were 30°C and 20°C, respectively. Immobilized artichoke PPOs greatly preserved their thermal stability which exists anyway. The catalytic efficiency value (Vmax/Km) of the alginate beads is approximately high as two-and-a-half folds of that of alginate+κ-carrageenan beads for cresolase activity. These values were very close for catecholase activity. Immobilized beads saved their both activities after 30 days of storage at 4°C.

  3. Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Shu, Xikai; Wang, Mei; Liu, Daicheng [College of Life Science, Shandong Normal University, Jinan, Shandong (China); Wang, Daijie; Lin, Xiaojing; Liu, Jianhua; Wang, Xiao; Huang, Luqi, E-mail: wxjn1998@126.com [Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong (China)

    2013-09-01

    An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-{beta}-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-{beta}-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). (author)

  4. Database Manager

    Science.gov (United States)

    Martin, Andrew

    2010-01-01

    It is normal practice today for organizations to store large quantities of records of related information as computer-based files or databases. Purposeful information is retrieved by performing queries on the data sets. The purpose of DATABASE MANAGER is to communicate to students the method by which the computer performs these queries. This…

  5. Maize microarray annotation database

    Directory of Open Access Journals (Sweden)

    Berger Dave K

    2011-10-01

    Full Text Available Abstract Background Microarray technology has matured over the past fifteen years into a cost-effective solution with established data analysis protocols for global gene expression profiling. The Agilent-016047 maize 44 K microarray was custom-designed from EST sequences, but only reporter sequences with EST accession numbers are publicly available. The following information is lacking: (a reporter - gene model match, (b number of reporters per gene model, (c potential for cross hybridization, (d sense/antisense orientation of reporters, (e position of reporter on B73 genome sequence (for eQTL studies, and (f functional annotations of genes represented by reporters. To address this, we developed a strategy to annotate the Agilent-016047 maize microarray, and built a publicly accessible annotation database. Description Genomic annotation of the 42,034 reporters on the Agilent-016047 maize microarray was based on BLASTN results of the 60-mer reporter sequences and their corresponding ESTs against the maize B73 RefGen v2 "Working Gene Set" (WGS predicted transcripts and the genome sequence. The agreement between the EST, WGS transcript and gDNA BLASTN results were used to assign the reporters into six genomic annotation groups. These annotation groups were: (i "annotation by sense gene model" (23,668 reporters, (ii "annotation by antisense gene model" (4,330; (iii "annotation by gDNA" without a WGS transcript hit (1,549; (iv "annotation by EST", in which case the EST from which the reporter was designed, but not the reporter itself, has a WGS transcript hit (3,390; (v "ambiguous annotation" (2,608; and (vi "inconclusive annotation" (6,489. Functional annotations of reporters were obtained by BLASTX and Blast2GO analysis of corresponding WGS transcripts against GenBank. The annotations are available in the Maize Microarray Annotation Database http://MaizeArrayAnnot.bi.up.ac.za/, as well as through a GBrowse annotation file that can be uploaded to

  6. Probabilistic Databases

    CERN Document Server

    Suciu, Dan; Koch, Christop

    2011-01-01

    Probabilistic databases are databases where the value of some attributes or the presence of some records are uncertain and known only with some probability. Applications in many areas such as information extraction, RFID and scientific data management, data cleaning, data integration, and financial risk assessment produce large volumes of uncertain data, which are best modeled and processed by a probabilistic database. This book presents the state of the art in representation formalisms and query processing techniques for probabilistic data. It starts by discussing the basic principles for rep

  7. Database Replication

    CERN Document Server

    Kemme, Bettina

    2010-01-01

    Database replication is widely used for fault-tolerance, scalability and performance. The failure of one database replica does not stop the system from working as available replicas can take over the tasks of the failed replica. Scalability can be achieved by distributing the load across all replicas, and adding new replicas should the load increase. Finally, database replication can provide fast local access, even if clients are geographically distributed clients, if data copies are located close to clients. Despite its advantages, replication is not a straightforward technique to apply, and

  8. RDD Databases

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database was established to oversee documents issued in support of fishery research activities including experimental fishing permits (EFP), letters of...

  9. Dealer Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The dealer reporting databases contain the primary data reported by federally permitted seafood dealers in the northeast. Electronic reporting was implemented May 1,...

  10. National database

    DEFF Research Database (Denmark)

    Kristensen, Helen Grundtvig; Stjernø, Henrik

    1995-01-01

    Artikel om national database for sygeplejeforskning oprettet på Dansk Institut for Sundheds- og Sygeplejeforskning. Det er målet med databasen at samle viden om forsknings- og udviklingsaktiviteter inden for sygeplejen.......Artikel om national database for sygeplejeforskning oprettet på Dansk Institut for Sundheds- og Sygeplejeforskning. Det er målet med databasen at samle viden om forsknings- og udviklingsaktiviteter inden for sygeplejen....

  11. Annotation of Ehux ESTs

    Energy Technology Data Exchange (ETDEWEB)

    Kuo, Alan; Grigoriev, Igor

    2009-06-12

    22 percent ESTs do no align with scaffolds. EST Pipeleine assembles 17126 consensi from the noaligned ESTs. Annotation Pipeline predicts 8564 ORFS on the consensi. Domain analysis of ORFs reveals missing genes. Cluster analysis reveals missing genes. Expression analysis reveals potential strain specific genes.

  12. 借助斑马鱼EST数据库从鲤鱼微卫星序列中寻找蛋白编码基因%Searching for Protein-coding Genes Using Microsatellites in Common Carp by Comparing to Zebrafish EST Database

    Institute of Scientific and Technical Information of China (English)

    常玉梅; 匡友谊; 梁利群; 鲁翠云; 何建国; 孙效文

    2008-01-01

    应用in sifico的方法,利用Blastu和Blastx搜索引擎,将鲤鱼微卫星序列与GenBank数据库进行同源序列比对.利用Blastn,将侧翼序列长度>50bp的875个鲤鱼微卫星序列与斑马鱼的EST数据库首先进行比对,结果找到了121个同源序列.随后采用Blastx搜索蛋白质数据库,有94个微卫星位点存在同源蛋白.除了33个假定和3个未知蛋白外,剩余的58个微卫星位点被成功地进行了功能注释,而且其中的7个位点已经定位在了鲤鱼连锁图谱上.另外,通过PCR-SSCP的方法,将两个与鲤鱼微卫星侧翼序列相匹配的斑马鱼EST序列开发成鲤鱼的STS标记,并将其中的一个标记HLJZe33定位到鲤鱼连锁图谱上.以上研究结果表明,通过比较基因组研究,模式生物斑马鱼的很多遗传和基因组资源都可以被利用到鲤鱼的基因组研究中.%In this study,an in silico approach was utilized to identify homologies existing between common carp microsatellite sequences and GenBank database using Biastn and Blastx searches.About 875 microsatellite sites with flanking sequences over 50bp of common carp were first compared to the zebrafish EST database.The results showed that 121 homologies were found using Blastn.Subsequent Blastx searches confirmed 94 sites recorded in the protein database.Except for 33 hypothetical proteins and three unknown proteins,seven out of 58 characterized proteins have been mapped to two linkage maps.In addition,two polymorphic STS markers were developed using matched zebrafish EST sequences by PCR-SSCP method,of which one marker HLJZe33 was mapped successfully.This study Was a pilot for comparative studies between common carp and zebrafish,and the results demonstrated that more genetic and genomic resources of zebrafish can be used for the genome research of common carp.

  13. Biological Databases

    Directory of Open Access Journals (Sweden)

    Kaviena Baskaran

    2013-12-01

    Full Text Available Biology has entered a new era in distributing information based on database and this collection of database become primary in publishing information. This data publishing is done through Internet Gopher where information resources easy and affordable offered by powerful research tools. The more important thing now is the development of high quality and professionally operated electronic data publishing sites. To enhance the service and appropriate editorial and policies for electronic data publishing has been established and editors of article shoulder the responsibility.

  14. Action of two alkalating agents, ethyl methyl sulfonate and nitrogenous mustard, on Jerusalem artichoke tissues cultivated in vitro. Protection of these tissues by cysteamin and restoration by kinetin

    Energy Technology Data Exchange (ETDEWEB)

    Jonard, R.

    1968-01-01

    Methane methyl sulfonate and nitrogenous mustard cause an inhibition of the proliferation of the Jerusalem artichoke tissues grown in vitro. The cysteamin which protects those tissues from the effect of X-rays, acts in a protective way solely as regards the toxic effect of nitrogenous mustard. Kinetin, which is an efficient radiorestorer for those tissues, brings back a proliferation of the samples subjected to the effect of nitrogenous mustard. 13 references, 2 tables.

  15. Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid.

    Science.gov (United States)

    Wang, Ling-Fei; Wang, Zhi-Peng; Liu, Xiao-Yan; Chi, Zhen-Ming

    2013-11-01

    In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem artichoke tubers without addition of any other components were suitable for citric acid production by the yeast strain. During 10 L fermentation using the extract containing 84.3 g L(-1) total sugars, 68.3 g L(-1) citric acid was produced and the yield of citric acid was 0.91 g g(-1) within 336 h. At the end of the fermentation, 9.2 g L(-1) of residual total sugar and 2.1 g L(-1) of reducing sugar were left in the fermented medium. At the same time, citric acid in the supernatant of the culture was purified. It was found that 67.2 % of the citric acid in the supernatant of the culture was recovered and purity of citric acid in the crystal was 96 %.

  16. Separation and quantification of inulin in selected artichoke (Cynara scolymus L.) cultivars and dandelion (Taraxacum officinale WEB. ex WIGG.) roots by high-performance anion exchange chromatography with pulsed amperometric detection.

    Science.gov (United States)

    Schütz, Katrin; Muks, Erna; Carle, Reinhold; Schieber, Andreas

    2006-12-01

    The profile of fructooligosaccharides and fructopolysaccharides in artichoke heads and dandelion roots was investigated. For this purpose, a suitable method for high-performance anion exchange chromatography with pulsed amperometic detection was developed. The separation of monomers, oligomers and polymers up to a chain length of 79 sugar residues was achieved in one single run. Glucose, fructose, sucrose and individual fructooligosaccharides (kestose, nystose, fructofuranosylnystose) were quantified in six different artichoke cultivars and in dandelion roots. The contents ranged from 12.9 g/kg DM to 71.7 g/kg DM for glucose, from 15.8 g/kg DM to 67.2 g/kg DM for fructose, and from 16.8 g/kg DM to 55.2 g/kg DM for sucrose in the artichoke heads. Kestose was the predominant fructooligosaccharide, followed by nystose and fructofuranosylnystose. In four cultivars fructofuranosylnystose was only detectable in traces and reached its maximum value of 3.6 g/kg DM in the cultivar Le Castel. Furthermore, an average degree of polymerization of 5.3 to 16.7 was calculated for the individual artichoke cultivars, which is noticeably lower than hitherto reported. In contrast, the contents of kestose, nystose and fructofuranosylnystose in dandelion root exceeded that of artichoke, reflecting the short chain characteristic of the inulin, which was confirmed by chromatographic analysis.

  17. Transliteration in EST Translation

    Institute of Scientific and Technical Information of China (English)

    王新然

    2016-01-01

    Firstly, this paper presents the definition of transliteration and its important position in EST translation. Secondly, in terms of the previous practice and experience in EST translation, four main transliteration techniques are concluded and analyzed. But meanwhile, there are still some negative issues and phenomena. As a result, it is worthy to make good use of the existing transliteration techniques and create more proper ones to remove the obstructions and promote the development of EST translation.

  18. In silco mapping of ESTs from the turkey (Meleagris gallopavo).

    Science.gov (United States)

    Reed, Kent M; Knutson, Todd P; Krueth, Stacy B; Sullivan, Laura R; Chaves, Lee D

    2005-01-01

    Sequence similarity was used to predict the position of expressed sequence tags (ESTs) in the genome of the turkey (Meleagris gallopavo). Turkey EST sequences were compared with the draft assembly of the chicken whole-genome sequence and the chicken EST database by BLASTN. Among the 877 ESTs examined, 788 had significant matches in the chicken genome sequence. Position of orthologous sequences in the chicken genome and the predicted position of the EST loci in the turkey genome are presented Genetic assignments suggest a high level of accuracy for the COMPASS predictions.

  19. Optimization of microwave-assisted drying of Jerusalem artichokes (Helianthus tuberosus L. by response surface methodology and genetic algorithm

    Directory of Open Access Journals (Sweden)

    E. KARACABEY

    2016-03-01

    Full Text Available The objective of the present study was to investigate microwave-assisted drying of Jerusalem artichoke tubers to determine the effects of the processing conditions. Drying time (DT and effectivemoisture diffusivity (EMD were determined to evaluate the drying process in terms of dehydration performance, whereas the rehydration ratio (RhR was considered as a significant quality index. A pretreatment of soaking in a NaCl solution was applied before all trials. The output power of the microwave oven, slice thickness and NaCl concentration of the pretreatment solution werethe three investigated parameters. The drying process was accelerated by altering the conditions while obtaining a higher quality product. For optimization of the drying process, response surface methodology (RSM and genetic algorithms (GA were used. Model adequacy was evaluated for each corresponding mathematical expression developed for interested responses by RSM. The residual of the model obtained by GA was compared to that of the RSM model. The GA was successful in high-performance prediction and produced results similar to those of RSM. The analysis and results of the present study show that both RSM and GA models can be used in cohesion to gain insight into the bioprocessing system.

  20. Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase.

    Science.gov (United States)

    Kim, D M; Kim, H S

    1992-02-01

    Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chitin-immobilized inulinase in alginate beads. The optimum amounts of both chitin-immobilized inulinase and permeabilized cells for coimmobilization were determined, and operational conditions were optimized. In a continuous stirred tank reactor operation, the maximum productivities for gluconic acid and sorbitol were about 19.2 and 21.3 g/L/h, respectively, at the dilution rate of 0.23 h(-1) and the substrate concentration of 20%, but operational stability was low because of the abrasion of the beads. As an approach to increase the operational stability, a recycle packed-bed reactor (RPBR) was employed. In RPBR operation, the maximum productivities for gluconic acid and sorbitol were found to be 23.4 and 26.0 g/L/h, respectively, at the dilution rate of 0.35 h(-1) and the substrate concentration of 20% when the recirculation rate was fixed at 900 mL/h. Coimmobilized enzymes were stable for 250 h in a recycle packed-bed reactor without any loss of activity, while half-life in a continuous stirred tank reactor (CSTR) was observed to be about 150 h.

  1. A Two-Step Nanofiltration Process for the Production of Phenolic-Rich Fractions from Artichoke Aqueous Extracts

    Directory of Open Access Journals (Sweden)

    Alfredo Cassano

    2015-04-01

    Full Text Available Commercial nanofiltration (NF membranes in spiral-wound configuration (NP030 from Microdyn Nadir and Desal DK from GE Water & Process Technologies were used in a sequential design in order to produce a separated fraction of phenolic and sugar compounds from an aqueous artichoke extract. For both membranes, the effect of transmembrane pressure (TMP on the permeation flux was evaluated. In optimized conditions of TMP, the NP030 membrane exhibited high rejections of apigenin, cynarin and chlorogenic acid (higher than 85%; on the other hand, very low rejections of fructose, glucose and sucrose (lower than 4% were measured. Starting from an extract with a total antioxidant activity (TAA of 5.28 mM trolox a retentate fraction with a TAA of 47.75 mM trolox was obtained. The NF permeate from the NP030 membrane was processed with the Desal DK membrane in optimized conditions of TMP producing a permeate stream free of phenolic and sugar compounds. Accordingly, as most part of phenolic compounds was removed in the first NF step, the concentration of sugar compounds in the NF retentate had much higher results than that of phenolic compounds.

  2. Kinetics of ethanol production by immobilized Kluyveromyces marxianus cells at varying sugar concentrations of Jerusalem artichoke juice

    Energy Technology Data Exchange (ETDEWEB)

    Bajpai, P.; Margaritis, A.

    1987-08-01

    Kinetics of ethanol fermentation at varying sugar concentrations of Jerusalem artichoke tuber extract has been studied using Kluyveromyces marxianus cells immobilized in calcium alginate gel beads. A maximum ethanol concentration of 111 g/l was achieved at an initial sugar concentration of 260 g/l in 20 hours, when the immobilized cell concentration in the calcium alginate beads was 53.3 g dry wt./l bead volume. Ethanol yield remained almost unaffected by initial sugar concentration up to 250 g/l and was found to be about 88% of the theoretical. Maximum rate of ethanol production decreased from 22.5 g ethanol/l/h to 10.5 g ethanol/l/h while the maximum rate of total sugars utilization decreased from 74.9 g sugars/l/h to 28.5 g sugars/l/h as the initial substrate concentration was increased from 100 to 300 g/l. The concentration of free cells in the fermentation broth was low.

  3. Extraction of bioactive carbohydrates from artichoke (Cynara scolymus L.) external bracts using microwave assisted extraction and pressurized liquid extraction.

    Science.gov (United States)

    Ruiz-Aceituno, Laura; García-Sarrió, M Jesús; Alonso-Rodriguez, Belén; Ramos, Lourdes; Sanz, M Luz

    2016-04-01

    Microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) methods using water as solvent have been optimized by means of a Box-Behnken and 3(2) composite experimental designs, respectively, for the effective extraction of bioactive carbohydrates (inositols and inulin) from artichoke (Cynara scolymus L.) external bracts. MAE at 60 °C for 3 min of 0.3 g of sample allowed the extraction of slightly higher concentrations of inositol than PLE at 75 °C for 26.7 min (11.6 mg/g dry sample vs. 7.6 mg/g dry sample). On the contrary, under these conditions, higher concentrations of inulin were extracted with the latter technique (185.4 mg/g vs. 96.4 mg/g dry sample), considering two successive extraction cycles for both techniques. Both methodologies can be considered appropriate for the simultaneous extraction of these bioactive carbohydrates from this particular industrial by-product. To the best of our knowledge this is the first time that these techniques are applied for this purpose.

  4. Salinity altered root distribution and increased diversity of bacterial communities in the rhizosphere soil of Jerusalem artichoke

    Science.gov (United States)

    Yang, Hui; Hu, Jinxiang; Long, Xiaohua; Liu, Zhaopu; Rengel, Zed

    2016-02-01

    The interaction between roots and bacterial communities in halophytic species is poorly understood. Here, we used Jerusalem artichoke cultivar Nanyu 1 (NY-1) to characterise root distribution patterns and determine diversity and abundance of bacteria in the rhizosphere soil under variable salinity. Root growth was not inhibited within the salinity range 1.2 to 1.9 g salt/kg, but roots were mainly confined to 0–20 cm soil layer vertically and 0–30 cm horizontally from the plant centre. Root concentrations of K+, Na+, Mg2+ and particularly Ca2+ were relatively high under salinity stress. High salinity stress decreased soil invertase and catalase activity. Using a next-generation, Illumina-based sequencing approach, we determined higher diversity of bacteria in the rhizosphere soil at high than low salinity. More than 15,500 valid reads were obtained, and Proteobacteria, Acidobacteria, Bacteroidetes and Actinobacteria predominated in all samples, accounting for >80% of the reads. On a genus level, 636 genera were common to the low and high salinity treatments at 0–5 cm and 5–10 cm depth. The abundance of Steroidobacter and Sphingomonas was significantly decreased by increasing salinity. Higher Shannon and Chao 1 indices with increasing severity of salt stress indicated that high salt stress increased diversity in the bacterial communities.

  5. EFFECTS OF ARTICHOKE (CYNARA SCOLYMUS L. EXTRACT ADDITION ON MICROBIOLOGICAL AND PHYSICO-CHEMICAL PROPERTIES OF PROBIOTIC YOGURT

    Directory of Open Access Journals (Sweden)

    Jalal Ehsani

    2015-06-01

    Full Text Available In this study, the effects of addition of artichoke (Cynara scolymus L. leaf extract into yogurt (0 or 0.5% on biochemical parameters (pH, titrable acidity and the viability of probiotic bacteria (Lactobacillus acidophilus LA-5, Bifidobacterium lactis BB-12 during fermentation and over 28 days of refrigerated storage (4°C were investigated. Moreover, the amounts of syneresis, total phenolic content, antioxidant activity and sensory attributes of yogurts at the end of fermentation were assessed. Yogurts contained the two yogurt bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus: ABY or only S. thermophilus (ABT as adjunct culture to probiotics. Yogurts containing Cynara scolymus L. (ABT-C and ABY-C had faster acidity increase, shorter incubation time and greater final titrable acidity than control yogurts (ABT and ABY. Also, yogurts containing Cynara scolymus L. had lower syneresis, higher total phenolic content and greater antioxidant activity. ABT-C yogurt had the ever greatest viability of probiotics. In case of samples sensory evaluation, generally, the highest total score was related to ABT yogurt whereas lowest total score belonged to ABT-C yogurt.

  6. Effect of artichoke (Cynara scolymus L. by-product on the quality and total phenol content of bread

    Directory of Open Access Journals (Sweden)

    Maroua Boubaker

    2016-06-01

    Full Text Available Legume flours, due to their phenol and fibre content, are ideal ingredients for improving the nutritional value of bakery products. In this study, artichoke stem powder (ASP was used to substitute 0%, 2.5%, 5%, 7.5% and 10% of wheat flour for making breads. Proximate composition of wheat flour and ASP were determined. Bread qualities and total phenols content were analyzed and compared with those of wheat bread. Results show that ASP contained 10.37% moisture, 10.28% ash, 11.53% protein, 0.86% fat, 51.29% fibre and 1350 mg EAG/100g d.m. ASP addition considerably modified the bread quality: altered appearance and texture, darker crumb and more intense odour were observed. From the sensory evaluation, tastes of bread with higher content of ASP (7.5 and 10% were the most acceptable for assessors. Total phenol contents of breads significantly increased with the addition of ASP. Therefore ASP may be considered as valuable ingredients for industrial manufacture of functional foods.

  7. Nursery inoculation with the arbuscular mycorrhizal fungus Glomus viscosum and its effect on the growth and physiology of hybrid artichoke seedlings

    Directory of Open Access Journals (Sweden)

    Angela Campanelli

    2011-07-01

    Full Text Available Most nurseries operating in Italy adopt high technologies and produce transplants that well suit and satisfy the grower’s need to produce high value crops. Mycorrhizas are discussed as a tool for improving and developing plant production in the nursery. Much research has been carried out on mycorrhizal symbiosis and we now know more about the symbiontic relationship between fungi and host plants. Plants receive numerous benefits from this symbiosis which are more macroscopic the earlier in the ontogenetic cycle this symbiosis is established. Therefore, it appears that the most effective period in which the inoculum should be made corresponds to the in-nursery growing stage. The earlier the plant is inoculated, the more evident the effect will be. In this study, several aspects related to the physiological foundations of arbuscular mycorrhiza in artichoke plants are presented. The main goal was to study the effects of mycorrhiza on the growth and physiological parameters of three hybrids of artichokes growing in the nursery. The experimental 3¥2 design included two treatments (with or without arbuscular mycorrhizal fungi and three hybrids of artichokes marketed by Nunhems (Opal F1, Madrigal F1, Concerto F1. Mycorrhizal plants have greater shoot length, leaf area, shoot and root fresh and dry mass, and root density. This also corresponded with increased photosynthetic rates and stomatal conductance of mycorrhizal plants. Mycorrhizal colonization improves relative water content and increases proline concentration in vegetal tissue. Inoculation produced the most beneficial effect on hybrid Madrigal F1 and on hybrid Opal F1; the best mycorrhizal affinity was enhanced when compared to hybrid Concerto F1. The results showed that mycorrhizal symbiosis stimulated the growth of inoculated seedlings providing a qualitatively good propagation material.

  8. Database development and management

    CERN Document Server

    Chao, Lee

    2006-01-01

    Introduction to Database Systems Functions of a DatabaseDatabase Management SystemDatabase ComponentsDatabase Development ProcessConceptual Design and Data Modeling Introduction to Database Design Process Understanding Business ProcessEntity-Relationship Data Model Representing Business Process with Entity-RelationshipModelTable Structure and NormalizationIntroduction to TablesTable NormalizationTransforming Data Models to Relational Databases .DBMS Selection Transforming Data Models to Relational DatabasesEnforcing ConstraintsCreating Database for Business ProcessPhysical Design and Database

  9. 菊芋对四川泡菜储藏期间品质变化影响的研究%Study on the Effects of Jerusalem Artichoke on the Quality Changes in the Natural Fermentation of Pickle during Storage

    Institute of Scientific and Technical Information of China (English)

    刘晓莉

    2012-01-01

    Jerusalem artichoke was rich in amino acid, sugar and vitamin, and used for curing pickles or pickled made. Merchant artichoke, Chinese cabbage, radish as raw materials in the salt concentration was 6% , under natural fer-mentation conditions, the comparison experiment of pickle contend Jerusalem artichoke and without was carried out. The results showed that adding the Jerusalem artichoke had little effects on the pH value and total acidity, but increased the ni-trite and ammonia nitrogen content significantly.%菊芋富含氨基酸、糖分和维生素,多用来腌制咸菜或制成泡菜。以市售菊芋、白菜、萝b为原料在食盐浓度为6%、自然发酵条件下的含菊芋的泡菜和不含菊芋的泡菜进行比较实验,实验结果表明:添加菊芋对泡菜pH值、总酸度影响不大,明显增加了亚硝酸盐和氨态氮含量。

  10. Effect of Chlorocholine Chloride (CCC on the Plants’ Height and Inulin Content in Jerusalem Artichoke (Helianthus tuberosus L.

    Directory of Open Access Journals (Sweden)

    Mikołaj Wawrzyniak

    2016-11-01

    Full Text Available Jerusalem artichoke (Helianthus tuberosus L. is herbaceous perennial plant rich in inulin and useful source of biomass. Due to its low agricultural requirements and high adaptability, it can provide very high biomass yields even on low quality sites. The plant is used in food industry, bio-fuel production, forage, pharmacy and nutrition. Its tubers accumulate approx. 10-20% of inulin in fresh weight. Currently, the use of the Helianthius tuberosus L. as a potential dietary strategy in patients affected by type 2 Diabetes is challenge. Moreover, deep understanding of the relationship between diet and composition of gut microbiota can bring the new insight in the treatment of inflammatory dependent diseases. The aim of this study was to examine an effect of plant growth retardant Chlorocholine Chloride (CCC on the plants’ height of H. tuberosus and inulin content in the tubers. We examined in the field a procedure for its shoots reduction. Material for the experiment were bought in a Polish commercial company and 528 tubers were planted in field in the middle of April 2014. Then, half of them were sprayed with 0.75% retardant of CCC . Furthermore, every week for 12 following weeks, the plants’ heights were measured. After the vegetation was over, 6 tubers for each treatment were dug out and chemically analyzed for inulin content using High Pressure Size Exclusion Chromatography. After first week of CCC use, 16% decrease of the heights plants was observed. Height of plants sprayed with CCC were significantly different comparing to Control. Weekly growth was significantly  slower in plants sprayed with CCC on first three weeks after applying retardant. Differences in plants height sustain to the end of measurements. Used retardant and its concentration did not affect the inulin content of the tubers.

  11. Databases and their application

    NARCIS (Netherlands)

    E.C. Grimm; R.H.W Bradshaw; S. Brewer; S. Flantua; T. Giesecke; A.M. Lézine; H. Takahara; J.W.,Jr Williams

    2013-01-01

    During the past 20 years, several pollen database cooperatives have been established. These databases are now constituent databases of the Neotoma Paleoecology Database, a public domain, multiproxy, relational database designed for Quaternary-Pliocene fossil data and modern surface samples. The poll

  12. Study of self-pollination and capitula characteristics in globe artichoke (Cynara cardunculus var. scolymus Hayek L. under different irrigation regimes

    Directory of Open Access Journals (Sweden)

    Nouraei, Sina

    2016-07-01

    Full Text Available In order to estimate the drought effects on capitula characteristics and self-pollination of globe artichoke (Cynara cardunculus var. scolymus Hayek L., the randomized complete block design was carried out with three irrigation regimes (20 %, 50 % and 80 % depletion of soil available water and six replicates. The artichoke is mostly open-pollinated, however, after covering the buds and isolation of flowers to prevent cross pollination, 1.79 % self-pollination was observed and this amount was not affected by different irrigation regimes. In stress conditions (50 % and 80 % water depletion as well as non-stress condition (20 % water depletion, plants with respectively one and two medium capitula and without small capitula had most relative frequencies in the population and drought stress increased these relative frequencies by reducing the number of medium and small capitula in plants. In addition, Capitula size and dry weight were significantly affected by water stress. Water shortage induced severe decrease in length and dry weight of all capitula including large, medium and small, although capitula width was less affected by water deficit and only slight decline in medium (12.5 % and small capitula (23.7 % was observed under severe stress condition.

  13. Effect of Saline Aquaculture Effluent on Salt-Tolerant Jerusalem Artichoke (Helianthus tuberosus L.) in a Semi-Arid Coastal Area of China

    Institute of Scientific and Technical Information of China (English)

    ZHAO Geng-Mao; LIU Zhao-Pu; CHEN Ming-Da; KOU Wei-Feng

    2006-01-01

    An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly,higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal

  14. 盐碱土壤对菊芋菊糖含量的影响%Effects of Saline-alkali Soils on Inulin Content in Jerusalem artichokes

    Institute of Scientific and Technical Information of China (English)

    周东; 隋丹; 于涛; 蔺吉祥

    2014-01-01

    为明确盐碱土壤对菊芋(Helianthus tuberosus )中菊糖含量的影响,利用高效液相色谱法对农田土壤与盐碱土壤上生长的7个品系菊芋的菊糖含量进行分析,盐碱土壤包括轻度盐碱土壤与重度盐碱土壤。结果表明:生长于盐碱土壤的菊芋的菊糖含量均低于农田土壤,其中菊芋品系2、品系5和品系7在轻度盐碱土壤中生长时菊糖含量高于重度盐碱土壤;菊芋品系1、品系4和品系6在重度盐碱土壤中生长时菊糖含量高于轻度盐碱土壤。7个品系中,品系4的菊糖含量在盐碱土壤下降低最多,品系6的菊糖含量在盐碱土壤下降低最少,但其含量在三种生长条件下是所有品系中最低的;品系5的菊糖含量虽然在盐碱土壤下也有所降低,但其含量在三种生长条件下都是所有品系中最高的,推测品系5比较适合在盐碱土壤上生长,在改良盐碱地时可优先选用。%To clarify the effects of saline soils on inulin content in Jerusalem artichokes,we analyze the inulin content of seven strains of Jerusalem artichokes grown on farmland and saline soils,which in-clude mild saline soils and severe saline soils by HPLC.The results show that the inulin content in Je-rusalem artichokes grown on the saline soils is lower than that on the farmland.It is also found that the inulin content in Jerusalem artichokes strain 2,strain 5,and strain 7 grown on the mild saline soils is higher than that on the severe saline soils and the inulin content in Jerusalem artichokes strain 1,strain 4,strain 6 grown on the severe saline soils is higher than that on the mild saline soil.The in-ulin content of strain 4 declines the most on the saline soils in the seven strains.The inulin content of strain 6 declines the least on the saline soils,but its inulin content is the lowest in the seven strains under the three growing conditions.Although the inulin content of strain 5 also declines on the saline

  15. Collecting Taxes Database

    Data.gov (United States)

    US Agency for International Development — The Collecting Taxes Database contains performance and structural indicators about national tax systems. The database contains quantitative revenue performance...

  16. NoSQL Databases

    OpenAIRE

    2013-01-01

    This thesis deals with database systems referred to as NoSQL databases. In the second chapter, I explain basic terms and the theory of database systems. A short explanation is dedicated to database systems based on the relational data model and the SQL standardized query language. Chapter Three explains the concept and history of the NoSQL databases, and also presents database models, major features and the use of NoSQL databases in comparison with traditional database systems. In the fourth ...

  17. USAID Anticorruption Projects Database

    Data.gov (United States)

    US Agency for International Development — The Anticorruption Projects Database (Database) includes information about USAID projects with anticorruption interventions implemented worldwide between 2007 and...

  18. AcEST: BP914202 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acid

  19. AcEST: DK945742 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945742|Adiantum...J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  20. AcEST: DK952487 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3...hang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  1. AcEST: DK945762 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3...hang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  2. AcEST: DK957377 [AcEST

    Lifescience Database Archive (English)

    Full Text Available eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957377|Adiantum capil...man (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  3. AcEST: DK948732 [AcEST

    Lifescience Database Archive (English)

    Full Text Available f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948..., and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  4. AcEST: BP911825 [AcEST

    Lifescience Database Archive (English)

    Full Text Available eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Q...iller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  5. AcEST: DK945381 [AcEST

    Lifescience Database Archive (English)

    Full Text Available f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945..., and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  6. AcEST: DK946712 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic

  7. AcEST: DK945511 [AcEST

    Lifescience Database Archive (English)

    Full Text Available a new generation of protein database search programs, Nucleic Acids Res. 25:3389... Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  8. AcEST: DK946879 [AcEST

    Lifescience Database Archive (English)

    Full Text Available new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946879|Adiantu... J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  9. AcEST: DK946819 [AcEST

    Lifescience Database Archive (English)

    Full Text Available LAST: a new generation of protein database search programs, Nucleic Acids Res. 25...Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search program

  10. AcEST: BP914772 [AcEST

    Lifescience Database Archive (English)

    Full Text Available -BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9147...avid J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  11. AcEST: DK958618 [AcEST

    Lifescience Database Archive (English)

    Full Text Available . Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids

  12. AcEST: BP917801 [AcEST

    Lifescience Database Archive (English)

    Full Text Available n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917801|Adiantum capillus-ve...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,

  13. AcEST: DK945340 [AcEST

    Lifescience Database Archive (English)

    Full Text Available w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402...g, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  14. 菊芋中菊糖提取与纯化工艺研究%Study on Extraction and Purification Technology of Inulin from Jerusalem Artichokes

    Institute of Scientific and Technical Information of China (English)

    王振强; 申森; 张耀光

    2011-01-01

    Study that the extraction and purification of jerusalem artichoke. First, the drying method of the artichoke slices that was being dried in the oven by hot air at 60 ℃ was indicated. And the extraction temperature, the extraction time, the solid to liquid ratio, the extraction times were selected for the singlefactor test. Second, the range of conditions was determined, the extraction conditions were optimized by orthogonal experiment, the optimal conditions of the inulin extraction from jerusalem artichoke were obtained, the extraction temperature was 70 ℃, the extraction time was 70 min, the solid to liquid ratio was 1 : 30, the extraction times was two, and the inulin yield rate was up to 52.80%. Others, the best treatment conditions of protein removal by milk of lime were determined, water bath temperature was between 70 ℃ and 80 ℃, water bath time was from 60 min to 90 min, with the best effect of depigmentation by macroporous adsorptive resins S-8 and the low rate of inulin loss.%研究了菊芋中菊糖的提取与纯化,确定了菊芋片干燥方法为60℃烘箱热风烘干。选择提取温度、提取时间、料液比、提取次数进行单因素试验,确定条件范围,再采用正交试验优化提取条件,得到菊芋中菊糖提取最佳工艺条件为温度70℃,提取时间70min,料液比1:30,提取次数2次,菊糖得率可达52.80%。并确定了石灰乳法脱蛋白最佳处理条件为水浴温度70℃~80℃,水浴时间60min-90min,大孔吸附树脂S.8的脱色素效果最好,菊糖损失率较低。

  15. 微波法提取菊芋中菊糖的工艺研究%Microwave Extraction of Inulin from Jerusalem artichoke

    Institute of Scientific and Technical Information of China (English)

    贾若凌

    2012-01-01

    [ Objective] To optimize the microwave extraction technology of inulin from J. artichoke. [ Method] With fresh J. artichoke tuber as experimental materials, based on a single factor experiment, five factors of microwave power, extraction time, solid - liquid ratio, extraction frequency and extraction temperature were selected to perform an orthogonal experiment L16(45) at four levels to study the best extraction conditions of J. artichoke. [ Result] The best extraction conditions were 8 min of microwave time, 95 ℃ of extraction temperature, 500 W of microwave power, 1: 20( W/V,g/ml) of solid-liquid ratio and three times of extraction frequency; under the best conditions, the extraction rate of inulin could reach up to 68.11%. [Conclusion] The method optimized the microwave extraction conditions of inulin, and the results were accurate and practical.%[目的]优选菊芋中菊糖的微波提取工艺.[方法]以新鲜的菊芋块茎为原料,在单因素试验的基础上,以微波功率、提取时间、料液比、提取次数和提取温度为因素,设计5因素4水平的正交试验L16 (45),研究菊糖的提取及其分离纯化的最佳工艺条件.[结果]确定微波法提取菊糖的最佳工艺条件为:微波时间8 min,提取温度95℃,微波功率500W,料液比为1∶20(W/V,g/ml,下同),提取3次;在此条件下,菊芋葡糖的提取率为68.11%.[结论]该方法优选出了菊芋中菊糖的微波提取工艺,结果准确可行.

  16. Cloud Databases: A Paradigm Shift in Databases

    Directory of Open Access Journals (Sweden)

    Indu Arora

    2012-07-01

    Full Text Available Relational databases ruled the Information Technology (IT industry for almost 40 years. But last few years have seen sea changes in the way IT is being used and viewed. Stand alone applications have been replaced with web-based applications, dedicated servers with multiple distributed servers and dedicated storage with network storage. Cloud computing has become a reality due to its lesser cost, scalability and pay-as-you-go model. It is one of the biggest changes in IT after the rise of World Wide Web. Cloud databases such as Big Table, Sherpa and SimpleDB are becoming popular. They address the limitations of existing relational databases related to scalability, ease of use and dynamic provisioning. Cloud databases are mainly used for data-intensive applications such as data warehousing, data mining and business intelligence. These applications are read-intensive, scalable and elastic in nature. Transactional data management applications such as banking, airline reservation, online e-commerce and supply chain management applications are write-intensive. Databases supporting such applications require ACID (Atomicity, Consistency, Isolation and Durability properties, but these databases are difficult to deploy in the cloud. The goal of this paper is to review the state of the art in the cloud databases and various architectures. It further assesses the challenges to develop cloud databases that meet the user requirements and discusses popularly used Cloud databases.

  17. Logical database design principles

    CERN Document Server

    Garmany, John; Clark, Terry

    2005-01-01

    INTRODUCTION TO LOGICAL DATABASE DESIGNUnderstanding a Database Database Architectures Relational Databases Creating the Database System Development Life Cycle (SDLC)Systems Planning: Assessment and Feasibility System Analysis: RequirementsSystem Analysis: Requirements Checklist Models Tracking and Schedules Design Modeling Functional Decomposition DiagramData Flow Diagrams Data Dictionary Logical Structures and Decision Trees System Design: LogicalSYSTEM DESIGN AND IMPLEMENTATION The ER ApproachEntities and Entity Types Attribute Domains AttributesSet-Valued AttributesWeak Entities Constraint

  18. Production of a single cyclic type of fructooligosaccharide structure by inulin-degrading Paenibacillus sp. LX16 newly isolated from Jerusalem artichoke root.

    Science.gov (United States)

    Yao, Zhihua; Guo, Jiqiang; Tang, Wenzhu; Sun, Zhen; Hou, Yingmin; Li, Xianzhen

    2016-05-01

    A novel inulin-degrading bacterium was isolated from a soil sample collected on Jerusalem artichoke roots. It is a Gram-positive, aerobic, motile and central endospore-forming straight rod, and exhibits phenotypic properties being consistent with its classification in the genus Paenibacillus. The predominant cellular fatty acids were anteiso-C15:0, C16:0 and anteiso-C17:0. This strain represents a novel species of the genus Paenibacillus on the basis of phenotypic data together with phylogenetic analysis, and it is here designated as LX16 and deposited in China centre for type collection, China (= CCTCC 2015256). Strain LX16 could produce a cyclofructooligosaccharide fructanotransferase catalysing the formation of one type of fructooligosaccharide (FOS) from inulin. The FOS was identified as a cyclofructooligosaccharide with a degree of polymerization of 6. Such homology in inulin degradation products may be beneficial for the functional FOS production.

  19. 酒曲应用于菊芋发酵的初步研究%A preliminary study fermentation of Jerusalem artichoke with koji

    Institute of Scientific and Technical Information of China (English)

    冯迪; 隆小华; 辛本荣; 刘兆普

    2011-01-01

    Jerusalem artichoke is a kind of alternative ethanol fermentation of raw materials. Compared with starch materials,the temperature of paste for Jerusalem artichoke is lower,which has the advantage in saving energy.Anqi liquor-making koji, Anqi Liqueur Koji and Xinma Liquor Industry Koji were used to study by one-step fermentation experiment and demonstrate the feasibility of producing ethanol fermentation of Jerusalem artichoke.The results were as follows:In the low raw sugar concentration and general treated conditions ,the alcohol content of Anqi liquor-making koji was higher than the other two koji, up to 5.5 degrees. Fermentation process, the weight loss of carbon dioxide of Anqi liquor-making koji was always higher than the other two koji. Use Anqi liquor-making koji to ferment,the ethanol concentration increased with the experimental time lasting during three days. With the further extension of the fermentation time, pH value of its broth was 6.35. pH value fell to 4.82 after 1 day fermentation and 4.48 after 3 days later,then pH value of the trend was stable. It can be concluded that compared with the other two koji, Anqi liquor-making koji was more suitable for bioethanol production using Jerusalem artichoke.%菊芋是一种极具潜力的乙醇发酵原料,与淀粉质原料相比,菊芋低温条件下即可实现糊化,在节能方面具有优势.本文选取了安琪甜酒曲、安琪酿酒曲和欣马酒业酒曲进行一步法发酵实验,论证不同种类及品种的酒曲应用于菊芋发酵生产乙醇的可行性.结果表明:在原料含糖量不高且未经任何条件优化的情况下,安琪酿酒曲的酒度高于其他两种酒曲,酒度可达5.5度;发酵过程中,安琪酿酒曲的CO2失重量始终高于安琪甜酒曲和欣马酒曲;使用安琪酿酒曲进行发酵,第1d到第3d乙醇含量逐渐升高,在第3d时达到最大值;随着发酵时间的继续延长,安琪酿酒曲发酵液PH为6.35.发酵1d后pH降至4.82,3d后pH降至4

  20. Screening pharmaceutical preparations containing extracts of turmeric rhizome, artichoke leaf, devil's claw root and garlic or salmon oil for antioxidant capacity.

    Science.gov (United States)

    Betancor-Fernández, Alejandro; Pérez-Gálvez, Antonio; Sies, Helmut; Stahl, Wilhelm

    2003-07-01

    Pharmaceutical preparations derived from natural sources such as vegetables often contain compounds that contribute to the antioxidant defence system and apparently play a role in the protection against degenerative diseases. In the present study, commercial preparations containing extracts of turmeric, artichoke, devil's claw and garlic or salmon oil were investigated. The products were divided into fractions of different polarity, and their antioxidant activity was determined using the Trolox equivalent antioxidant capacity (TEAC) assay. This test is based on the efficacy of the test material to scavenge 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) derived radicals. Total phenols were determined in all fractions as well as specific carotenoids in the most lipophilic fraction to assess their contribution to the antioxidant activity. For comparison, the radical scavenging effect of selected constituents of the extracts such as curcumin, luteolin, kaempferol, chlorogenic acid, harpagoside, beta-carotene and alpha-tocopherol was investigated and compared with that of Trolox. Curcumin, luteolin, kaempferol, chlorogenic acid and beta-carotene showed an antioxidant activity superior to Trolox in the TEAC assay; harpagoside was barely active. All fractions of the turmeric extract preparation exhibited pronounced antioxidant activity, which was assigned to the presence of curcumin and other polyphenols. The antioxidant activity corresponding to the artichoke leaf extract was higher in the aqueous fractions than in the lipophilic fractions. Similarly, devil's claw extract was particularly rich in water-soluble antioxidants. Harpagoside, a major compound in devil's claw, did not contribute significantly to its antioxidant activity. The antioxidant capacity of the garlic preparation was poor in the TEAC assay. That of salmon oil was mainly attributed to vitamin E, which is added to the product for stabilization. In all test preparations, the antioxidant

  1. The Effect of Ginger (Zingiber officinalis and Artichoke (Cynara cardunculus Extract Supplementation on Functional Dyspepsia: A Randomised, Double-Blind, and Placebo-Controlled Clinical Trial

    Directory of Open Access Journals (Sweden)

    Attilio Giacosa

    2015-01-01

    Full Text Available Objective. Functional dyspepsia (FD is a frequent clinical finding in western world. The aim of this study is to compare the efficacy of a ginger and artichoke supplementation versus placebo in the treatment of FD. Methods. A prospective multicentre, double blind, randomized, placebo controlled, parallel-group comparison of the supplement and placebo over a period of 4 weeks was performed. Two capsules/day were supplied (before lunch and dinner to 126 FD patients (supplementation/placebo: 65/61. Results. After 14 days of treatment, only supplementation group (SG showed a significant amelioration (SG: αS=+1.195 MCA score units (u, P=0.017; placebo: αP=+0.347 u, P=0.513. The intercept (α resulted to be significantly higher in SG than in placebo (αS-αP=+0.848 u, P<0.001. At the end of the study, the advantage of SG versus placebo persists without variation (βS-βP=+0.077 u, P=0.542. In SG, a significant advantage is observed for nausea (βS-βP=-0.398 u, P<0.001, epigastric fullness (βS-βP=-0.241, P<0.001, epigastric pain (βS-βP=-0.173 u, P=0.002, and bloating (βS-βP=-0.167 u, P=0.017. Conclusions. The association between ginger and artichoke leaf extracts appears safe and efficacious in the treatment of FD and could represent a promising treatment for this disease.

  2. Database Urban Europe

    NARCIS (Netherlands)

    Sleutjes, B.; de Valk, H.A.G.

    2016-01-01

    Database Urban Europe: ResSegr database on segregation in The Netherlands. Collaborative research on residential segregation in Europe 2014–2016 funded by JPI Urban Europe (Joint Programming Initiative Urban Europe).

  3. Cell Centred Database (CCDB)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Cell Centered Database (CCDB) is a web accessible database for high resolution 2D, 3D and 4D data from light and electron microscopy, including correlated imaging.

  4. Physiological Information Database (PID)

    Science.gov (United States)

    EPA has developed a physiological information database (created using Microsoft ACCESS) intended to be used in PBPK modeling. The database contains physiological parameter values for humans from early childhood through senescence as well as similar data for laboratory animal spec...

  5. E3 Staff Database

    Data.gov (United States)

    US Agency for International Development — E3 Staff database is maintained by E3 PDMS (Professional Development & Management Services) office. The database is Mysql. It is manually updated by E3 staff as...

  6. Scopus database: a review.

    Science.gov (United States)

    Burnham, Judy F

    2006-03-08

    The Scopus database provides access to STM journal articles and the references included in those articles, allowing the searcher to search both forward and backward in time. The database can be used for collection development as well as for research. This review provides information on the key points of the database and compares it to Web of Science. Neither database is inclusive, but complements each other. If a library can only afford one, choice must be based in institutional needs.

  7. MPlus Database system

    Energy Technology Data Exchange (ETDEWEB)

    1989-01-20

    The MPlus Database program was developed to keep track of mail received. This system was developed by TRESP for the Department of Energy/Oak Ridge Operations. The MPlus Database program is a PC application, written in dBase III+'' and compiled with Clipper'' into an executable file. The files you need to run the MPLus Database program can be installed on a Bernoulli, or a hard drive. This paper discusses the use of this database.

  8. Compressing DNA sequence databases with coil

    Directory of Open Access Journals (Sweden)

    Hendy Michael D

    2008-05-01

    Full Text Available Abstract Background Publicly available DNA sequence databases such as GenBank are large, and are growing at an exponential rate. The sheer volume of data being dealt with presents serious storage and data communications problems. Currently, sequence data is usually kept in large "flat files," which are then compressed using standard Lempel-Ziv (gzip compression – an approach which rarely achieves good compression ratios. While much research has been done on compressing individual DNA sequences, surprisingly little has focused on the compression of entire databases of such sequences. In this study we introduce the sequence database compression software coil. Results We have designed and implemented a portable software package, coil, for compressing and decompressing DNA sequence databases based on the idea of edit-tree coding. coil is geared towards achieving high compression ratios at the expense of execution time and memory usage during compression – the compression time represents a "one-off investment" whose cost is quickly amortised if the resulting compressed file is transmitted many times. Decompression requires little memory and is extremely fast. We demonstrate a 5% improvement in compression ratio over state-of-the-art general-purpose compression tools for a large GenBank database file containing Expressed Sequence Tag (EST data. Finally, coil can efficiently encode incremental additions to a sequence database. Conclusion coil presents a compelling alternative to conventional compression of flat files for the storage and distribution of DNA sequence databases having a narrow distribution of sequence lengths, such as EST data. Increasing compression levels for databases having a wide distribution of sequence lengths is a direction for future work.

  9. AcEST: DK946405 [AcEST

    Lifescience Database Archive (English)

    Full Text Available clone: YMU02A01NGRL0012_I07, 5' (164 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEM...(164 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  10. AcEST: DK943583 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 02A01NGRL0003_E17, 5' (136 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (releas...rs) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  11. AcEST: DK944932 [AcEST

    Lifescience Database Archive (English)

    Full Text Available , clone: YMU02A01NGRL0007_K08, 5' (157 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765... total letters Searching..................................................done ***** No hits found ****** Tr...' (157 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  12. AcEST: DK946057 [AcEST

    Lifescience Database Archive (English)

    Full Text Available e: YMU02A01NGRL0011_E24, 5' (185 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (... letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  13. AcEST: DK947500 [AcEST

    Lifescience Database Archive (English)

    Full Text Available one: YMU02A01NGRL0016_B17, 5' (128 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL...28 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  14. AcEST: DK946802 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 01NGRL0013_M17, 5' (172 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 3... Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  15. AcEST: DK943876 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 01NGRL0004_D18, 5' (151 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 3... Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  16. AcEST: DK947207 [AcEST

    Lifescience Database Archive (English)

    Full Text Available A01NGRL0015_C17, 5' (136 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release ...) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  17. AcEST: DK944150 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 02A01NGRL0005_C04, 5' (173 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (releas...rs) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  18. AcEST: DK946721 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 02A01NGRL0013_I10, 5' (138 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (releas...rs) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  19. AcEST: DK947414 [AcEST

    Lifescience Database Archive (English)

    Full Text Available , clone: YMU02A01NGRL0015_N04, 5' (168 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765... total letters Searching..................................................done ***** No hits found ****** Tr...' (168 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  20. AcEST: DK952775 [AcEST

    Lifescience Database Archive (English)

    Full Text Available clone: TST38A01NGRL0015_A07, 5' (170 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEM...(170 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  1. AcEST: DK945145 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1NGRL0008_G06, 5' (136 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39...Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  2. AcEST: DK946346 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU02A01NGRL0012_F04, 5' (108 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (re...etters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  3. AcEST: BP913388 [AcEST

    Lifescience Database Archive (English)

    Full Text Available iller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-34

  4. AcEST: DK944295 [AcEST

    Lifescience Database Archive (English)

    Full Text Available SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res...grams, Nucleic Acids Res. 25:3389-3402. Query= DK944295|...ng, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search pro

  5. AcEST: DK946772 [AcEST

    Lifescience Database Archive (English)

    Full Text Available -BLAST: a new generation of protein database search programs, Nucleic Acids Res. ..., Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progr...ams, Nucleic Acids Res. 25:3389-3402. Query= DK946772|Ad

  6. AcEST: DK944084 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3...eng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  7. AcEST: DK944792 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids R...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94479...hang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  8. AcEST: BP917157 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91715... Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  9. AcEST: DK945539 [AcEST

    Lifescience Database Archive (English)

    Full Text Available BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2... Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progra...ms, Nucleic Acids Res. 25:3389-3402. Query= DK945539|Adi

  10. AcEST: BP911599 [AcEST

    Lifescience Database Archive (English)

    Full Text Available SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91...eng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  11. AcEST: DK946525 [AcEST

    Lifescience Database Archive (English)

    Full Text Available I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946..., and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= DK946525|Adiantum capillus-veneris mR

  12. AcEST: BP921436 [AcEST

    Lifescience Database Archive (English)

    Full Text Available and PSI-BLAST: a new generation of protein database search programs, Nucleic Acid...g, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= BP921436|A

  13. AcEST: DK943809 [AcEST

    Lifescience Database Archive (English)

    Full Text Available avid J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nuc...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Qu

  14. AcEST: DK944876 [AcEST

    Lifescience Database Archive (English)

    Full Text Available a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944876|Adian...id J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  15. AcEST: DK943845 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Gapped BLAST and PSI-BLAST: a new generation of protein database search programs...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943845|Adiantum capillus-veneris...ler, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  16. AcEST: DK946127 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids R...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK94612...hang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of

  17. AcEST: DK950468 [AcEST

    Lifescience Database Archive (English)

    Full Text Available and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950468|Adiantum capillus-ven

  18. AcEST: DK946060 [AcEST

    Lifescience Database Archive (English)

    Full Text Available PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Re...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946060...ang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of p

  19. AcEST: BP914015 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914015|Adiantum cap

  20. AcEST: DK956634 [AcEST

    Lifescience Database Archive (English)

    Full Text Available d David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, ...grams, Nucleic Acids Res. 25:3389-3402. Query= DK956634|...ng, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search pro

  1. AcEST: DK945348 [AcEST

    Lifescience Database Archive (English)

    Full Text Available T: a new generation of protein database search programs, Nucleic Acids Res. 25:33...ng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  2. AcEST: DK947829 [AcEST

    Lifescience Database Archive (English)

    Full Text Available David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, N...g, Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search prog...rams, Nucleic Acids Res. 25:3389-3402. Query= DK947829|A

  3. AcEST: BP920861 [AcEST

    Lifescience Database Archive (English)

    Full Text Available er, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402.

  4. AcEST: BP917904 [AcEST

    Lifescience Database Archive (English)

    Full Text Available eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917904|Adiantum capil...ler, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

  5. CTD_DATABASE - Cascadia tsunami deposit database

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Cascadia Tsunami Deposit Database contains data on the location and sedimentological properties of tsunami deposits found along the Cascadia margin. Data have...

  6. Keyword Search in Databases

    CERN Document Server

    Yu, Jeffrey Xu; Chang, Lijun

    2009-01-01

    It has become highly desirable to provide users with flexible ways to query/search information over databases as simple as keyword search like Google search. This book surveys the recent developments on keyword search over databases, and focuses on finding structural information among objects in a database using a set of keywords. Such structural information to be returned can be either trees or subgraphs representing how the objects, that contain the required keywords, are interconnected in a relational database or in an XML database. The structural keyword search is completely different from

  7. An Interoperable Cartographic Database

    Directory of Open Access Journals (Sweden)

    Slobodanka Ključanin

    2007-05-01

    Full Text Available The concept of producing a prototype of interoperable cartographic database is explored in this paper, including the possibilities of integration of different geospatial data into the database management system and their visualization on the Internet. The implementation includes vectorization of the concept of a single map page, creation of the cartographic database in an object-relation database, spatial analysis, definition and visualization of the database content in the form of a map on the Internet. 

  8. The NCBI Taxonomy database.

    Science.gov (United States)

    Federhen, Scott

    2012-01-01

    The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the sequences represented in the INSDC's nucleotide and protein sequence databases. The taxonomy database is manually curated by a small group of scientists at the NCBI who use the current taxonomic literature to maintain a phylogenetic taxonomy for the source organisms represented in the sequence databases. The taxonomy database is a central organizing hub for many of the resources at the NCBI, and provides a means for clustering elements within other domains of NCBI web site, for internal linking between domains of the Entrez system and for linking out to taxon-specific external resources on the web. Our primary purpose is to index the domain of sequences as conveniently as possible for our user community.

  9. AcEST: BP920571 [AcEST

    Lifescience Database Archive (English)

    Full Text Available tein homolog OS=... 34 0.62 sp|Q9Y239|NOD1_HUMAN Nucleotide-binding oligomerization domain-c... 33 1.1 sp|Q5FWL4|EST2A_XENLA Extended...ITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRII 951 >sp|Q5FWL4|EST2A_XENLA Extended synaptotagmin-2-A OS=Xenopu

  10. Database Description - DGBY | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us ...EF URLs by Artio About This Database Database Description Download License Update History of This Database Site Policy | Contact Us Database Description - DGBY | LSDB Archive ...

  11. Database Description - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RMG Database... Description General information of database Database name RMG Alternative name Rice Mitochondri...ational Institute of Agrobiological Sciences E-mail : Database classification Nucleotide Sequence Databases ...Organism Taxonomy Name: Oryza sativa Japonica Group Taxonomy ID: 39947 Database description This database co...e of rice mitochondrial genome and information on the analysis results. Features and manner of utilization of database

  12. Database design and database administration for a kindergarten

    OpenAIRE

    Vítek, Daniel

    2009-01-01

    The bachelor thesis deals with creation of database design for a standard kindergarten, installation of the designed database into the database system Oracle Database 10g Express Edition and demonstration of the administration tasks in this database system. The verification of the database was proved by a developed access application.

  13. Estás contratado!

    OpenAIRE

    2012-01-01

    Este Objeto de aprendizaje interactivo muestra la simulación de una entrevista de trabajo. En este objeto se deberá elegir la opción de respuesta más adecuada de acuerdo con la pregunta planteada, recibiendo de manera inmediata las respuestas correctas y equivocadas.

  14. AcEST: BP915056 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 65 total letters Searching..................................................done ***** No hits found ****** ...72 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  15. AcEST: BP920979 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ,765 total letters Searching..................................................done ***** No hits found *****...(238 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  16. AcEST: BP919856 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000130_A02 514 Adiantum capillus-veneris mRNA. clone: YMU001_000130_A02. BP919856 - Show BP91985...is mRNA. clone: YMU001_000130_A02. Accession BP919856 Tissue type prothallium Developmental stage - Contig I...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91985... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919856|Adiantum c

  17. AcEST: BP914187 [AcEST

    Lifescience Database Archive (English)

    Full Text Available d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= B... Zheng Zhang, Webb Miller, and David J. Lipman (1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search progra...ms, Nucleic Acids Res. 25:3389-3402. Query= BP914187|Adiantum capillus-veneris mRNA

  18. AcEST: BP919887 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000130_C10 511 Adiantum capillus-veneris mRNA. clone: YMU001_000130_C10. BP919887 - Show BP91988...is mRNA. clone: YMU001_000130_C10. Accession BP919887 Tissue type prothallium Developmental stage - Contig I...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91988...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91988

  19. Conditioning Probabilistic Databases

    CERN Document Server

    Koch, Christoph

    2008-01-01

    Past research on probabilistic databases has studied the problem of answering queries on a static database. Application scenarios of probabilistic databases however often involve the conditioning of a database using additional information in the form of new evidence. The conditioning problem is thus to transform a probabilistic database of priors into a posterior probabilistic database which is materialized for subsequent query processing or further refinement. It turns out that the conditioning problem is closely related to the problem of computing exact tuple confidence values. It is known that exact confidence computation is an NP-hard problem. This has lead researchers to consider approximation techniques for confidence computation. However, neither conditioning nor exact confidence computation can be solved using such techniques. In this paper we present efficient techniques for both problems. We study several problem decomposition methods and heuristics that are based on the most successful search techn...

  20. Hazard Analysis Database Report

    CERN Document Server

    Grams, W H

    2000-01-01

    The Hazard Analysis Database was developed in conjunction with the hazard analysis activities conducted in accordance with DOE-STD-3009-94, Preparation Guide for U S . Department of Energy Nonreactor Nuclear Facility Safety Analysis Reports, for HNF-SD-WM-SAR-067, Tank Farms Final Safety Analysis Report (FSAR). The FSAR is part of the approved Authorization Basis (AB) for the River Protection Project (RPP). This document describes, identifies, and defines the contents and structure of the Tank Farms FSAR Hazard Analysis Database and documents the configuration control changes made to the database. The Hazard Analysis Database contains the collection of information generated during the initial hazard evaluations and the subsequent hazard and accident analysis activities. The Hazard Analysis Database supports the preparation of Chapters 3 ,4 , and 5 of the Tank Farms FSAR and the Unreviewed Safety Question (USQ) process and consists of two major, interrelated data sets: (1) Hazard Analysis Database: Data from t...

  1. National Database of Geriatrics

    DEFF Research Database (Denmark)

    Kannegaard, Pia Nimann; Vinding, Kirsten L; Hare-Bruun, Helle

    2016-01-01

    AIM OF DATABASE: The aim of the National Database of Geriatrics is to monitor the quality of interdisciplinary diagnostics and treatment of patients admitted to a geriatric hospital unit. STUDY POPULATION: The database population consists of patients who were admitted to a geriatric hospital unit....... Geriatric patients cannot be defined by specific diagnoses. A geriatric patient is typically a frail multimorbid elderly patient with decreasing functional ability and social challenges. The database includes 14-15,000 admissions per year, and the database completeness has been stable at 90% during the past......, percentage of discharges with a rehabilitation plan, and the part of cases where an interdisciplinary conference has taken place. Data are recorded by doctors, nurses, and therapists in a database and linked to the Danish National Patient Register. DESCRIPTIVE DATA: Descriptive patient-related data include...

  2. Judy Estes Hall (1940-2015).

    Science.gov (United States)

    Sammons, Morgan T; Boucher, Andrew

    2016-01-01

    Presents an obituary for Judy Estes Hall, who passed away on November 24, 2015. Hall served as the Executive Officer of the National Register of Health Service Psychologists until her retirement in 2013. She is a recognized expert in the development of education and training standards for the profession of psychology, she also made significant contributions in the field of international psychology, where she was a renowned expert in cross-national credentialing and an advocate for commonality in licensing standards. She was the coauthor of one edited volume and author of more than 60 journal articles, book chapters, and professional publications. A passionate advocate for the advancement of women in psychology, a devoted mother and grandmother, a connoisseur of wine and international traveler extraordinaire, she touched the personal and professional lives of many. (PsycINFO Database Record

  3. Searching Databases with Keywords

    Institute of Scientific and Technical Information of China (English)

    Shan Wang; Kun-Long Zhang

    2005-01-01

    Traditionally, SQL query language is used to search the data in databases. However, it is inappropriate for end-users, since it is complex and hard to learn. It is the need of end-user, searching in databases with keywords, like in web search engines. This paper presents a survey of work on keyword search in databases. It also includes a brief introduction to the SEEKER system which has been developed.

  4. Specialist Bibliographic Databases

    OpenAIRE

    Gasparyan, Armen Yuri; Yessirkepov, Marlen; Voronov, Alexander A.; Trukhachev, Vladimir I.; Kostyukova, Elena I.; Gerasimov, Alexey N.; Kitas, George D.

    2016-01-01

    Specialist bibliographic databases offer essential online tools for researchers and authors who work on specific subjects and perform comprehensive and systematic syntheses of evidence. This article presents examples of the established specialist databases, which may be of interest to those engaged in multidisciplinary science communication. Access to most specialist databases is through subscription schemes and membership in professional associations. Several aggregators of information and d...

  5. ITS-90 Thermocouple Database

    Science.gov (United States)

    SRD 60 NIST ITS-90 Thermocouple Database (Web, free access)   Web version of Standard Reference Database 60 and NIST Monograph 175. The database gives temperature -- electromotive force (emf) reference functions and tables for the letter-designated thermocouple types B, E, J, K, N, R, S and T. These reference functions have been adopted as standards by the American Society for Testing and Materials (ASTM) and the International Electrotechnical Commission (IEC).

  6. Smart Location Database - Download

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Smart Location Database (SLD) summarizes over 80 demographic, built environment, transit service, and destination accessibility attributes for every census block...

  7. Smart Location Database - Service

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Smart Location Database (SLD) summarizes over 80 demographic, built environment, transit service, and destination accessibility attributes for every census block...

  8. IVR EFP Database

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This database contains trip-level reports submitted by vessels participating in Exempted Fishery projects with IVR reporting requirements.

  9. Transporter Classification Database (TCDB)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Transporter Classification Database details a comprehensive classification system for membrane transport proteins known as the Transporter Classification (TC)...

  10. Database principles programming performance

    CERN Document Server

    O'Neil, Patrick

    2014-01-01

    Database: Principles Programming Performance provides an introduction to the fundamental principles of database systems. This book focuses on database programming and the relationships between principles, programming, and performance.Organized into 10 chapters, this book begins with an overview of database design principles and presents a comprehensive introduction to the concepts used by a DBA. This text then provides grounding in many abstract concepts of the relational model. Other chapters introduce SQL, describing its capabilities and covering the statements and functions of the programmi

  11. Database Publication Practices

    DEFF Research Database (Denmark)

    Bernstein, P.A.; DeWitt, D.; Heuer, A.

    2005-01-01

    There has been a growing interest in improving the publication processes for database research papers. This panel reports on recent changes in those processes and presents an initial cut at historical data for the VLDB Journal and ACM Transactions on Database Systems.......There has been a growing interest in improving the publication processes for database research papers. This panel reports on recent changes in those processes and presents an initial cut at historical data for the VLDB Journal and ACM Transactions on Database Systems....

  12. Residency Allocation Database

    Data.gov (United States)

    Department of Veterans Affairs — The Residency Allocation Database is used to determine allocation of funds for residency programs offered by Veterans Affairs Medical Centers (VAMCs). Information...

  13. Veterans Administration Databases

    Science.gov (United States)

    The Veterans Administration Information Resource Center provides database and informatics experts, customer service, expert advice, information products, and web technology to VA researchers and others.

  14. High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus

    Directory of Open Access Journals (Sweden)

    Bianco Linda

    2009-11-01

    Full Text Available Abstract Background In recent years, different HIV antigens have been successfully expressed in plants by either stable transformation or transient expression systems. Among HIV proteins, Nef is considered a promising target for the formulation of a multi-component vaccine due to its implication in the first steps of viral infection. Attempts to express Nef as a single protein product (not fused to a stabilizing protein in transgenic plants resulted in disappointingly low yields (about 0.5% of total soluble protein. In this work we describe a transient expression system based on co-agroinfiltration of plant virus gene silencing suppressor proteins in Nicotiana benthamiana, followed by a two-step affinity purification protocol of plant-derived Nef. Results The effect of three gene silencing viral suppressor proteins (P25 of Potato Virus X, P19 of either Artichoke Mottled Crinckle virus and Tomato Bushy Stunt virus on Nef transient expression yield was evaluated. The P19 protein of Artichoke Mottled Crinckle virus (AMCV-P19 gave the highest expression yield in vacuum co-agroinfiltration experiments reaching 1.3% of total soluble protein, a level almost three times higher than that previously reported in stable transgenic plants. The high yield observed in the co-agroinfiltrated plants was correlated to a remarkable decrease of Nef-specific small interfering RNAs (siRNAs indicating an effective modulation of RNA silencing mechanisms by AMCV-P19. Interestingly, we also showed that expression levels in top leaves of vacuum co-agroinfiltrated plants were noticeably reduced compared to bottom leaves. Moreover, purification of Nef from agroinfiltrated tissue was achieved by a two-step immobilized metal ion affinity chromatography protocol with yields of 250 ng/g of fresh tissue. Conclusion We demonstrated that expression level of HIV-1 Nef in plant can be improved using a transient expression system enhanced by the AMCV-P19 gene silencing suppressor

  15. An overview of researches on Jerusalem artichoke as a biofuel crop%菊芋作为能源植物的研究进展

    Institute of Scientific and Technical Information of China (English)

    刘祖昕; 谢光辉

    2012-01-01

    Non-food biofuel crop is under the spotlight in the research field of bioenergy.Jerusalem artichoke is one of the most promising non-food biofuel crops,due to its wide adaptability,high resistance,high biomass productivity,multiple ways of utilization for bioenergy and environment friendly.This paper reviews the recent development and problems in research in biology characteristics,stress resistance and yield potential,germplasm evaluation,genetic characteristics and cultivar breeding,planting technology,chemical composition and energy conversion on the basis of feedstock production and biomass conversion utilization.It discusses the prospects of research and development of Jerusalem artichoke as an energy crop.Specific cultivar for bioenergy,efficient feedstock production system suited for bioenergy plants,searching for inulinase with high activity,efficient fermentation strains,oleaginous microorganisms,life cycle assessment of feedstock production,and comprehensive research and development of byproducts should be focused.%非粮能源植物是发展生物质能源的重要基础。菊芋适应性广,抗逆性强,生物质产量高,能源化利用方式多样,且环境友好,是我国有前景的非粮能源植物之一。笔者以原料生产和生物质转化利用为中心,对菊芋生物学、抗逆性和产量潜力、种质资源、遗传特性和良种选育、种植技术、化学组成和能源转化利用等方面的研究进展和存在问题进行了详细阐述,并展望了菊芋作为能源植物的研发前景。培育能源专用良种,建立适应能源生产的高效生产技术体系、获得高活力菊糖酶、高效发酵菌株和产油微生物,原料生产全生命周期评价及副产物综合开发利用是今后应着力研究的重点。

  16. ESTs and EST-linked polymorphisms for genetic mapping and phylogenetic reconstruction in the guppy, Poecilia reticulata

    Directory of Open Access Journals (Sweden)

    Tripathi Namita

    2007-08-01

    Full Text Available Abstract Background The guppy, Poecilia reticulata, is a well-known model organism for studying inheritance and variation of male ornamental traits as well as adaptation to different river habitats. However, genomic resources for studying this important model were not previously widely available. Results With the aim of generating molecular markers for genetic mapping of the guppy, cDNA libraries were constructed from embryos and different adult organs to generate expressed sequence tags (ESTs. About 18,000 ESTs were annotated according to BLASTN and BLASTX results and the sequence information from the 3' UTRs was exploited to generate PCR primers for re-sequencing of genomic DNA from different wild type strains. By comparison of EST-linked genomic sequences from at least four different ecotypes, about 1,700 polymorphisms were identified, representing about 400 distinct genes. Two interconnected MySQL databases were built to organize the ESTs and markers, respectively. A robust phylogeny of the guppy was reconstructed, based on 10 different nuclear genes. Conclusion Our EST and marker databases provide useful tools for genetic mapping and phylogenetic studies of the guppy.

  17. A Quality System Database

    Science.gov (United States)

    Snell, William H.; Turner, Anne M.; Gifford, Luther; Stites, William

    2010-01-01

    A quality system database (QSD), and software to administer the database, were developed to support recording of administrative nonconformance activities that involve requirements for documentation of corrective and/or preventive actions, which can include ISO 9000 internal quality audits and customer complaints.

  18. Balkan Vegetation Database

    NARCIS (Netherlands)

    Vassilev, Kiril; Pedashenko, Hristo; Alexandrova, Alexandra; Tashev, Alexandar; Ganeva, Anna; Gavrilova, Anna; Gradevska, Asya; Assenov, Assen; Vitkova, Antonina; Grigorov, Borislav; Gussev, Chavdar; Filipova, Eva; Aneva, Ina; Knollová, Ilona; Nikolov, Ivaylo; Georgiev, Georgi; Gogushev, Georgi; Tinchev, Georgi; Pachedjieva, Kalina; Koev, Koycho; Lyubenova, Mariyana; Dimitrov, Marius; Apostolova-Stoyanova, Nadezhda; Velev, Nikolay; Zhelev, Petar; Glogov, Plamen; Natcheva, Rayna; Tzonev, Rossen; Boch, Steffen; Hennekens, Stephan M.; Georgiev, Stoyan; Stoyanov, Stoyan; Karakiev, Todor; Kalníková, Veronika; Shivarov, Veselin; Russakova, Veska; Vulchev, Vladimir

    2016-01-01

    The Balkan Vegetation Database (BVD; GIVD ID: EU-00-019; http://www.givd.info/ID/EU-00- 019) is a regional database that consists of phytosociological relevés from different vegetation types from six countries on the Balkan Peninsula (Albania, Bosnia and Herzegovina, Bulgaria, Kosovo, Montenegro

  19. HIV Structural Database

    Science.gov (United States)

    SRD 102 HIV Structural Database (Web, free access)   The HIV Protease Structural Database is an archive of experimentally determined 3-D structures of Human Immunodeficiency Virus 1 (HIV-1), Human Immunodeficiency Virus 2 (HIV-2) and Simian Immunodeficiency Virus (SIV) Proteases and their complexes with inhibitors or products of substrate cleavage.

  20. Biological Macromolecule Crystallization Database

    Science.gov (United States)

    SRD 21 Biological Macromolecule Crystallization Database (Web, free access)   The Biological Macromolecule Crystallization Database and NASA Archive for Protein Crystal Growth Data (BMCD) contains the conditions reported for the crystallization of proteins and nucleic acids used in X-ray structure determinations and archives the results of microgravity macromolecule crystallization studies.

  1. Children's Culture Database (CCD)

    DEFF Research Database (Denmark)

    Wanting, Birgit

    a Dialogue inspired database with documentation, network (individual and institutional profiles) and current news , paper presented at the research seminar: Electronic access to fiction, Copenhagen, November 11-13, 1996......a Dialogue inspired database with documentation, network (individual and institutional profiles) and current news , paper presented at the research seminar: Electronic access to fiction, Copenhagen, November 11-13, 1996...

  2. Structural Ceramics Database

    Science.gov (United States)

    SRD 30 NIST Structural Ceramics Database (Web, free access)   The NIST Structural Ceramics Database (WebSCD) provides evaluated materials property data for a wide range of advanced ceramics known variously as structural ceramics, engineering ceramics, and fine ceramics.

  3. Neutrosophic Relational Database Decomposition

    OpenAIRE

    Meena Arora; Ranjit Biswas; Dr. U.S.Pandey

    2011-01-01

    In this paper we present a method of decomposing a neutrosophic database relation with Neutrosophic attributes into basic relational form. Our objective is capable of manipulating incomplete as well as inconsistent information. Fuzzy relation or vague relation can only handle incomplete information. Authors are taking the Neutrosophic Relational database [8],[2] to show how imprecise data can be handled in relational schema.

  4. World Database of Happiness

    NARCIS (Netherlands)

    R. Veenhoven (Ruut)

    1995-01-01

    textabstractABSTRACT The World Database of Happiness is an ongoing register of research on subjective appreciation of life. Its purpose is to make the wealth of scattered findings accessible, and to create a basis for further meta-analytic studies. The database involves four sections:
    1. Bib

  5. Atomic Spectra Database (ASD)

    Science.gov (United States)

    SRD 78 NIST Atomic Spectra Database (ASD) (Web, free access)   This database provides access and search capability for NIST critically evaluated data on atomic energy levels, wavelengths, and transition probabilities that are reasonably up-to-date. The NIST Atomic Spectroscopy Data Center has carried out these critical compilations.

  6. BBGD: an online database for blueberry genomic data

    Directory of Open Access Journals (Sweden)

    Matthews Benjamin F

    2007-01-01

    Full Text Available Abstract Background Blueberry is a member of the Ericaceae family, which also includes closely related cranberry and more distantly related rhododendron, azalea, and mountain laurel. Blueberry is a major berry crop in the United States, and one that has great nutritional and economical value. Extreme low temperatures, however, reduce crop yield and cause major losses to US farmers. A better understanding of the genes and biochemical pathways that are up- or down-regulated during cold acclimation is needed to produce blueberry cultivars with enhanced cold hardiness. To that end, the blueberry genomics database (BBDG was developed. Along with the analysis tools and web-based query interfaces, the database serves both the broader Ericaceae research community and the blueberry research community specifically by making available ESTs and gene expression data in searchable formats and in elucidating the underlying mechanisms of cold acclimation and freeze tolerance in blueberry. Description BBGD is the world's first database for blueberry genomics. BBGD is both a sequence and gene expression database. It stores both EST and microarray data and allows scientists to correlate expression profiles with gene function. BBGD is a public online database. Presently, the main focus of the database is the identification of genes in blueberry that are significantly induced or suppressed after low temperature exposure. Conclusion By using the database, researchers have developed EST-based markers for mapping and have identified a number of "candidate" cold tolerance genes that are highly expressed in blueberry flower buds after exposure to low temperatures.

  7. The LHCb configuration database

    CERN Document Server

    Abadie, L; Van Herwijnen, Eric; Jacobsson, R; Jost, B; Neufeld, N

    2005-01-01

    The aim of the LHCb configuration database is to store information about all the controllable devices of the detector. The experiment's control system (that uses PVSS ) will configure, start up and monitor the detector from the information in the configuration database. The database will contain devices with their properties, connectivity and hierarchy. The ability to store and rapidly retrieve huge amounts of data, and the navigability between devices are important requirements. We have collected use cases to ensure the completeness of the design. Using the entity relationship modelling technique we describe the use cases as classes with attributes and links. We designed the schema for the tables using relational diagrams. This methodology has been applied to the TFC (switches) and DAQ system. Other parts of the detector will follow later. The database has been implemented using Oracle to benefit from central CERN database support. The project also foresees the creation of tools to populate, maintain, and co...

  8. Reclamation research database

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    A reclamation research database was compiled to help stakeholders search publications and research related to the reclamation of Alberta's oil sands region. New publications are added to the database by the Cumulative Environmental Management Association (CEMA), a nonprofit association whose mandate is to develop frameworks and guidelines for the management of cumulative environmental effects in the oil sands region. A total of 514 research papers have been compiled in the database to date. Topics include recent research on hydrology, aquatic and terrestrial ecosystems, laboratory studies on biodegradation, and the effects of oil sands processing on micro-organisms. The database includes a wide variety of studies related to reconstructed wetlands as well as the ecological effects of hydrocarbons on phytoplankton and other organisms. The database format included information on research format availability, as well as information related to the author's affiliations. Links to external abstracts were provided where available, as well as details of source information.

  9. Cascadia Tsunami Deposit Database

    Science.gov (United States)

    Peters, Robert; Jaffe, Bruce; Gelfenbaum, Guy; Peterson, Curt

    2003-01-01

    The Cascadia Tsunami Deposit Database contains data on the location and sedimentological properties of tsunami deposits found along the Cascadia margin. Data have been compiled from 52 studies, documenting 59 sites from northern California to Vancouver Island, British Columbia that contain known or potential tsunami deposits. Bibliographical references are provided for all sites included in the database. Cascadia tsunami deposits are usually seen as anomalous sand layers in coastal marsh or lake sediments. The studies cited in the database use numerous criteria based on sedimentary characteristics to distinguish tsunami deposits from sand layers deposited by other processes, such as river flooding and storm surges. Several studies cited in the database contain evidence for more than one tsunami at a site. Data categories include age, thickness, layering, grainsize, and other sedimentological characteristics of Cascadia tsunami deposits. The database documents the variability observed in tsunami deposits found along the Cascadia margin.

  10. 响应面法优化菊芋菊糖的提取工艺%Response Surface Methodology for Optimizing Extraction of Inulin from Jerusalem Artichoke

    Institute of Scientific and Technical Information of China (English)

    李峰; 陈帅; 晏敏; 闫彬; 许程剑

    2015-01-01

    The method of heat leaching was used to extract inulin from jerusalem artichoke to improve the utilization rate,in which respones surface analysis was used to optimize extraction conditions. Based on the single factor experiments,the Central Composite design principles and Design-Expert.8.05 data ananlysis software were used to select three parameters of response surface design to optimize extraction process. The result showed the optimize extraction conditions:extraction time 50 min,extraction temperature 84℃,liquid to solid ratio 35,the extraction value was 89.26%.%为了提高菊芋菊糖的提取率,本文采用热浸提法对菊芋进行处理,并通过响应面法优化提取条件。在单因素实验的基础上,依据Central Composite中心组合设计原理,采用Design-Expert.8.05数据分析软件,对菊糖提取进行了三因素三水平的优化分析。确立最佳提取条件:浸提时间50min,浸提温度84益,液料比35,在此条件下蛋白质的提取理论值达到89.26%。

  11. Alkali-based pretreatments distinctively extract lignin and pectin for enhancing biomass saccharification by altering cellulose features in sugar-rich Jerusalem artichoke stem.

    Science.gov (United States)

    Li, Meng; Wang, Jun; Yang, Yuezhou; Xie, Guanghui

    2016-05-01

    Jerusalem artichoke (JA) has been known as a potential nonfood feedstock for biofuels. Based on systems analysis of total 59 accessions, both soluble sugar and ash could positively affect biomass digestibility after dilute sodium hydroxide pretreatment (A). In this study, one representative accession (HEN-3) was used to illustrate its enzymatic digestibility with pretreatments of ultrasonic-assisted dilute sodium hydroxide (B), alkaline peroxide (C), and ultrasonic-assisted alkaline peroxide (D). Pretreatment D exhibited the highest hexose release rate (79.4%) and total sugar yield (10.4 g/L), which were 2.4 and 2.6 times higher, respectively, than those of the control. The analysis of cellulose crystalline index (CrI), cellulose degree of polymerization (DP), thermal behavior and SEM suggested that alkali-based pretreatments could distinctively extract lignin and pectin polymers, leading to significant alterations of cellulose CrI and DP for high biomass saccharification. Additionally, hydrogen peroxide (H2O2) could significant reduce the generation of fermentation inhibitors during alkali-based pretreatments.

  12. Effect of temperature and pH on ethanol production by free and immobilized cells of Kluyveromyces marxianus grown on Jerusalem artichoke extract

    Energy Technology Data Exchange (ETDEWEB)

    Bajpai, P.; Margaritis, A.

    1987-01-01

    The effect of temperature and pH on the kinetics of ethanol production by free and calcium alginate immobilized cells of Kluyveromyces marxianus grown on Jerusalem artichoke extract was investigated. With the free cells, the ethanol and biomass yields were relatively constant over the temperature range 25-35 degrees C, but dropped sharply beyond 35 degrees C. Other kinetic parameters, specific growth rate, specific ethanol production rate, and specific total sugar uptake rate were maximum at 35 degrees C. However, with the immobilized cells, ethanol yield remained almost constant in the temperatue range 25-45 degrees C, and the specific ethanol production rate and specific total sugar uptake rate attained their maximum values at 40 degrees C. For the pH range between 3 and 7, the free-cell optimum for growth and product formation was found to be circa pH 5. At this pH, the specific growth rate was 0.35/h and specific ethanol production rate was 2.83 g/g/h. At values higher or lower than pH 5, a sharp decrease in specific ethanol production rate as well as specific growth rate was observed. In comparison, the immobilized cells showed a broad optimum pH profile. The best ethanol production rates were observed between pH 4 and 6. (Refs. 22).

  13. Ethanol production from Jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast Kluyveromyces marxianus using consolidated bioprocessing.

    Science.gov (United States)

    Charoensopharat, Kanlayani; Thanonkeo, Pornthap; Thanonkeo, Sudarat; Yamada, Mamoru

    2015-07-01

    Thermotolerant inulin-utilizing yeast strains were successfully isolated in this study. Among the isolated strains, Kluyveromyces marxianus DBKKU Y-102 was found to be the most effective strain for direct ethanol fermentation at high temperature from fresh Jerusalem artichoke (JA) tubers without inulin hydrolysis under consolidated bioprocessing (CBP). The maximum ethanol concentrations produced by this strain under the optimum culture conditions were 104.83 and 97.46 g L(-1) at 37 and 40 °C, respectively. Data from this study clearly demonstrated that the use of thermotolerant inulin-utilizing yeast K. marxianus for ethanol production from fresh JA tubers in the CBP process not only provided high levels of ethanol, but also could eliminate the addition of external enzyme for inulin hydrolysis, which might lead to the reduction of operating costs. The expression of genes involved in carbohydrate metabolism in K. marxianus DBKKU Y-102 during ethanol fermentation was investigated by real-time RT-PCR, and the results revealed that expression levels were distinctive depending on the growth phase and growth conditions. However, among the genes tested, adh4 and tdh2 were highly expressed under high temperature conditions in both exponential- and stationary-growth phases, suggesting that these genes might play a crucial role in acquiring thermotolerance ability in this organism under stress conditions.

  14. Selective production of 1,2-propylene glycol from Jerusalem artichoke tuber using Ni-W(2) C/AC catalysts.

    Science.gov (United States)

    Zhou, Likun; Wang, Aiqin; Li, Changzhi; Zheng, Mingyuan; Zhang, Tao

    2012-05-01

    A series of Ni-promoted W(2) C/activated carbon (AC) catalysts were investigated for the catalytic conversion of Jerusalem artichoke tuber (JAT) under hydrothermal conditions and hydrogen pressure. Even a small amount of Ni could greatly promote the conversion of JAT to 1,2-propylene glycol (1,2-PG), whereas the pure W(2) C/AC catalyst resulted in the selective formation of acetol. The product distribution profiles involving the reaction temperature, time, and H(2) pressure indicated that 1,2-PG formed as a result of acetol hydrogenation, which was catalyzed by Ni. Thus, there was a synergy between W(2) C and Ni, and the best performance yielded 38.5% of 1,2-PG over a 4%Ni-20%W(2) C/AC catalyst at 245°C, 6 MPa H(2) , and 80 min. To understand the reaction process, some important intermediates, such as inulin, fructose, acetol, glyceraldehyde, and 1,3-dihydroxyacetone, were used as the feedstock. Based on the product distributions derived from these intermediates, a reaction pathway was proposed, where JAT was first hydrolyzed into a mixture of fructose and glucose under the catalysis of H(+) , then the sugars underwent a retro-aldol reaction followed by hydrogenation catalyzed by Ni-W(2) C.

  15. AcEST: BP919858 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000130_A04 328 Adiantum capillus-veneris mRNA. clone: YMU001_000130_A04. BP919858 - Show BP91985...is mRNA. clone: YMU001_000130_A04. Accession BP919858 Tissue type prothallium Developmental stage - Contig I...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91985...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919858|Adiantum capillu

  16. AcEST: BP919857 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000130_A03 501 Adiantum capillus-veneris mRNA. clone: YMU001_000130_A03. BP91985...7 CL3173Contig1 Show BP919857 Clone id YMU001_000130_A03 Library YMU01 Length 501 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000130_A03. Accession BP919857 Tissue type prothallium Developmental stag...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91985...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919857|Ad

  17. AcEST: BP912912 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000024_C05 413 Adiantum capillus-veneris mRNA. clone: YMU001_000024_C05. BP912912... CL1433Contig1 Show BP912912 Clone id YMU001_000024_C05 Library YMU01 Length 413 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000024_C05. Accession BP912912 Tissue type prothallium Developmental stag... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912912|Adiantum capillus-ven...eris mRNA, clone: YMU001_000024_C05. (413 letters) Database: uniprot_sprot.fasta 412

  18. AcEST: BP912312 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000017_F01 489 Adiantum capillus-veneris mRNA. clone: YMU001_000017_F01. BP912312... CL1779Contig1 Show BP912312 Clone id YMU001_000017_F01 Library YMU01 Length 489 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000017_F01. Accession BP912312 Tissue type prothallium Developmental stag...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912312...|Adiantum capillus-veneris mRNA, clone: YMU001_000017_F01. (489 letters) Database: uniprot_sprot.fasta 412

  19. AcEST: BP912128 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D10 477 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D10. BP91212...8 CL2328Contig1 Show BP912128 Clone id YMU001_000015_D10 Library YMU01 Length 477 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000015_D10. Accession BP912128 Tissue type prothallium Developmental stag... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91212...8|Adiantum capillus-veneris mRNA, clone: YMU001_000015_D10. (461 letters) Database: uniprot_sprot.fasta 412

  20. AcEST: BP912212 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000016_D11 457 Adiantum capillus-veneris mRNA. clone: YMU001_000016_D11. BP912212... CL1085Contig1 Show BP912212 Clone id YMU001_000016_D11 Library YMU01 Length 457 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000016_D11. Accession BP912212 Tissue type prothallium Developmental stag...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912212...|Adiantum capillus-veneris mRNA, clone: YMU001_000016_D11. (457 letters) Database: uniprot_sprot.fasta 412

  1. AcEST: BP912123 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_D05 496 Adiantum capillus-veneris mRNA. clone: YMU001_000015_D05. BP91212...3 CL498Contig1 Show BP912123 Clone id YMU001_000015_D05 Library YMU01 Length 496 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000015_D05. Accession BP912123 Tissue type prothallium Developmental stage...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91212...3|Adiantum capillus-veneris mRNA, clone: YMU001_000015_D05. (478 letters) Database: uniprot_sprot.fasta 412

  2. AcEST: BP912017 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_A11 578 Adiantum capillus-veneris mRNA. clone: YMU001_000012_A11. BP912017... CL1368Contig1 Show BP912017 Clone id YMU001_000012_A11 Library YMU01 Length 578 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_A11. Accession BP912017 Tissue type prothallium Developmental stag... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912017|Adiant...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912017|Adiantum capillus-veneris mRNA

  3. AcEST: BP920175 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_H09 490 Adiantum capillus-veneris mRNA. clone: YMU001_000133_H09. BP920175 - Show BP92017...is mRNA. clone: YMU001_000133_H09. Accession BP920175 Tissue type prothallium Developmental stage - Contig I...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92017...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920175|Adiantum

  4. AcEST: BP920174 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_H08 486 Adiantum capillus-veneris mRNA. clone: YMU001_000133_H08. BP920174 - Show BP92017...is mRNA. clone: YMU001_000133_H08. Accession BP920174 Tissue type prothallium Developmental stage - Contig I... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92017...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92017

  5. AcEST: BP913037 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000025_F12 436 Adiantum capillus-veneris mRNA. clone: YMU001_000025_F12. BP913037 - Show BP91303...is mRNA. clone: YMU001_000025_F12. Accession BP913037 Tissue type prothallium Developmental stage - Contig I...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91303...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913037|Adiantum capillus-veneris mR

  6. AcEST: BP913031 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000025_F06 302 Adiantum capillus-veneris mRNA. clone: YMU001_000025_F06. BP91303...1 CL1500Contig1 Show BP913031 Clone id YMU001_000025_F06 Library YMU01 Length 302 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000025_F06. Accession BP913031 Tissue type prothallium Developmental stag...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91303...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91303

  7. AcEST: BP913303 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000028_G07 539 Adiantum capillus-veneris mRNA. clone: YMU001_000028_G07. BP913303 - Show BP913303...is mRNA. clone: YMU001_000028_G07. Accession BP913303 Tissue type prothallium Developmental stage - Contig I... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913303...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913303

  8. AcEST: BP914303 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_D07 644 Adiantum capillus-veneris mRNA. clone: YMU001_000057_D07. BP914303... CL2270Contig1 Show BP914303 Clone id YMU001_000057_D07 Library YMU01 Length 644 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000057_D07. Accession BP914303 Tissue type prothallium Developmental stag... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914303|Adiantum capillus-veneri...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914303

  9. AcEST: DK958303 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK958303 - Show DK958303 Clone id TST39A01NGRL0030_K10 Library TST39 Length 634 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0030_K10. 5' end sequence. Accession DK958303 Tissue t... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK958303|Adiant...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK958303

  10. AcEST: BP920303 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000135_E01 493 Adiantum capillus-veneris mRNA. clone: YMU001_000135_E01. BP920303 - Show BP920303...is mRNA. clone: YMU001_000135_E01. Accession BP920303 Tissue type prothallium Developmental stage - Contig I...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920303...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920303|Adiantum capillus-

  11. AcEST: BP918043 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_A02 493 Adiantum capillus-veneris mRNA. clone: YMU001_000109_A02. BP91804...3 CL3885Contig1 Show BP918043 Clone id YMU001_000109_A02 Library YMU01 Length 493 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000109_A02. Accession BP918043 Tissue type prothallium Developmental stag... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91804...-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91804

  12. AcEST: BP918044 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_A03 150 Adiantum capillus-veneris mRNA. clone: YMU001_000109_A03. BP918044 - Show BP91804...is mRNA. clone: YMU001_000109_A03. Accession BP918044 Tissue type prothallium Developmental stage - Contig I...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91804...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918044|Adiantum cap

  13. AcEST: BP918047 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_A06 349 Adiantum capillus-veneris mRNA. clone: YMU001_000109_A06. BP91804...7 CL1525Contig1 Show BP918047 Clone id YMU001_000109_A06 Library YMU01 Length 349 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000109_A06. Accession BP918047 Tissue type prothallium Developmental stag...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918047|Adiantum capillus-veneris mRNA...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918047|Adian

  14. AcEST: BP918048 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_A07 409 Adiantum capillus-veneris mRNA. clone: YMU001_000109_A07. BP918048 - Show BP91804...is mRNA. clone: YMU001_000109_A07. Accession BP918048 Tissue type prothallium Developmental stage - Contig I...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91804... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91804

  15. AcEST: BP918046 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_A05 481 Adiantum capillus-veneris mRNA. clone: YMU001_000109_A05. BP91804...6 CL10Contig1 Show BP918046 Clone id YMU001_000109_A05 Library YMU01 Length 481 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000109_A05. Accession BP918046 Tissue type prothallium Developmental stage ...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918046|Adi...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918046|Adiantum capillus-v

  16. AcEST: DK956260 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK956260 CL494Contig1 Show DK956260 Clone id TST39A01NGRL0025_E11 Library TST39 Length 6...07 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_E11. 5' end sequence. Accession DK956260...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956260|Adiantum capi...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956260|Adiantum ca

  17. AcEST: DK948260 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK948260 CL524Contig1 Show DK948260 Clone id TST38A01NGRL0002_N15 Library TST38 Length 6...59 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0002_N15. 5' end sequence. Accession DK948260... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948260...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948260|Adiantum capillus-veneris mRNA

  18. AcEST: DK952604 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK952604 CL2982Contig1 Show DK952604 Clone id TST38A01NGRL0014_I20 Library TST38 Length ...647 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_I20. 5' end sequence. Accession DK95260... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952604|Adiantum c...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95260...SYLPGWQKP----YKVVPYEDLH 259 Query: 259 WRFSQRLLDLMWIPMKWLAIPVFALS 336 WR ++ W+PMKWLAI F L+ Sbjct: 260 WRVTRP

  19. AcEST: BP916260 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000085_C09 277 Adiantum capillus-veneris mRNA. clone: YMU001_000085_C09. BP916260 - Show BP916260...is mRNA. clone: YMU001_000085_C09. Accession BP916260 Tissue type prothallium Developmental stage - Contig I...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916260|Adiantum capillus-vene...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916260

  20. AcEST: BP912600 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000020_G07 375 Adiantum capillus-veneris mRNA. clone: YMU001_000020_G07. BP91260...0 CL1497Contig1 Show BP912600 Clone id YMU001_000020_G07 Library YMU01 Length 375 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000020_G07. Accession BP912600 Tissue type prothallium Developmental stag...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912600|Adiantum capillus-vener...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912600|Adiantum capillus-vener

  1. AcEST: DK961101 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK961101 CL398Contig1 Show DK961101 Clone id TST39A01NGRL0009_D07 Library TST39 Length 6...81 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_D07. 5' end sequence. Accession DK961101...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961101|Ad...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961101...TST39A01NGRL0009_D07 681 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0009_D0

  2. AcEST: BP911984 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000011_F07 566 Adiantum capillus-veneris mRNA. clone: YMU001_000011_F07. BP911984... CL2332Contig1 Show BP911984 Clone id YMU001_000011_F07 Library YMU01 Length 566 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000011_F07. Accession BP911984 Tissue type prothallium Developmental stag...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911984|Adiantum capillus-veneris mR...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911984

  3. AcEST: BP919843 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000129_G09 473 Adiantum capillus-veneris mRNA. clone: YMU001_000129_G09. BP91984...3 CL2697Contig1 Show BP919843 Clone id YMU001_000129_G09 Library YMU01 Length 473 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000129_G09. Accession BP919843 Tissue type prothallium Developmental stag...ein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919843|Ad...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919843|Adiantum capillus-veneris

  4. AcEST: DK944403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK944403 - Show DK944403 Clone id YMU02A01NGRL0005_P03 Library YMU02 Length 271 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_P03. 5' end sequence. Accession DK944403 Tissue t...7), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944403|Adian

  5. AcEST: DK954403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK954403 - Show DK954403 Clone id TST39A01NGRL0020_F14 Library TST39 Length 534 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_F14. 5' end sequence. Accession DK954403 Tissue t..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954403...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954403|A

  6. AcEST: DK962403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK962403 CL3688Contig1 Show DK962403 Clone id TST39A01NGRL0013_M11 Library TST39 Length ...630 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_M11. 5' end sequence. Accession DK962403...apped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962403...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962403|Adiantum capillus-veneris mRNA

  7. AcEST: DK956403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK956403 CL70Contig1 Show DK956403 Clone id TST39A01NGRL0025_K10 Library TST39 Length 55...9 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_K10. 5' end sequence. Accession DK956403...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956403|Adiantum capillus-veneris ...itives = 25/39 (64%) Frame = +1 Query: 403 FDPLGLGKDPAALKWYREAEIIHGRWAMAAVVGIFVGQA 519 FDPLG KDPA + + EI +GR...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956403

  8. AcEST: BP914037 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_B09 437 Adiantum capillus-veneris mRNA. clone: YMU001_000039_B09. BP914037 - Show BP91403...is mRNA. clone: YMU001_000039_B09. Accession BP914037 Tissue type prothallium Developmental stage - Contig I... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914037|Adia... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914037|Adia

  9. AcEST: BP914033 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_B04 449 Adiantum capillus-veneris mRNA. clone: YMU001_000039_B04. BP914033 - Show BP91403...is mRNA. clone: YMU001_000039_B04. Accession BP914033 Tissue type prothallium Developmental stage - Contig I... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91403...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25

  10. AcEST: BP914039 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000039_B11 577 Adiantum capillus-veneris mRNA. clone: YMU001_000039_B11. BP91403...9 CL791Contig1 Show BP914039 Clone id YMU001_000039_B11 Library YMU01 Length 577 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000039_B11. Accession BP914039 Tissue type prothallium Developmental stage... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914039|Adiantum capillus-ven...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91403

  11. AcEST: DK951403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK951403 - Show DK951403 Clone id TST38A01NGRL0011_F03 Library TST38 Length 670 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_F03. 5' end sequence. Accession DK951403 Tissue t..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951403...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951403|Adiantu

  12. AcEST: BP916403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000087_C10 523 Adiantum capillus-veneris mRNA. clone: YMU001_000087_C10. BP916403... CL144Contig1 Show BP916403 Clone id YMU001_000087_C10 Library YMU01 Length 523 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000087_C10. Accession BP916403 Tissue type prothallium Developmental stage...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916403... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP916403|Adiantum c

  13. AcEST: DK957403 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK957403 - Show DK957403 Clone id TST39A01NGRL0028_E12 Library TST39 Length 623 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0028_E12. 5' end sequence. Accession DK957403 Tissue t...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957403|Adi...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957403|Adiantum capillus-vene

  14. AcEST: BP914444 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000059_A03 493 Adiantum capillus-veneris mRNA. clone: YMU001_000059_A03. BP914444... CL1628Contig1 Show BP914444 Clone id YMU001_000059_A03 Library YMU01 Length 493 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000059_A03. Accession BP914444 Tissue type prothallium Developmental stag...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914444... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914444|Adiantum capillus-veneri

  15. AcEST: DK953500 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK953500 - Show DK953500 Clone id TST39A01NGRL0017_O23 Library TST39 Length 480 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0017_O23. 5' end sequence. Accession DK953500 Tissue t...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953500

  16. AcEST: DK956500 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK956500 - Show DK956500 Clone id TST39A01NGRL0025_O12 Library TST39 Length 608 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0025_O12. 5' end sequence. Accession DK956500 Tissue t...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956500|Adiantum capillus-v...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK956500

  17. AcEST: DK950060 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0007_K13 702 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0007_K1...3. 5' end sequence. DK950060 CL316Contig1 Show DK950060 Clone id TST38A01NGRL0007_K13 Library TST38 Length 7...02 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0007_K13. 5' end sequence. Accession DK9500...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950060...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950060

  18. AcEST: DK961500 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK961500 CL37Contig1 Show DK961500 Clone id TST39A01NGRL0010_E20 Library TST39 Length 69...2 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_E20. 5' end sequence. Accession DK961500... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961500...LAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961500

  19. AcEST: DK950087 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0007_L16 709 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0007_L1...6. 5' end sequence. DK950087 - Show DK950087 Clone id TST38A01NGRL0007_L16 Library TST38 Length 709 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0007_L16. 5' end sequence. Accession DK950087 Tissue t...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950087|Adia...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK9500

  20. AcEST: BP920500 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000137_G11 581 Adiantum capillus-veneris mRNA. clone: YMU001_000137_G11. BP920500... CL3073Contig1 Show BP920500 Clone id YMU001_000137_G11 Library YMU01 Length 581 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000137_G11. Accession BP920500 Tissue type prothallium Developmental stag...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920500...ration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920500|Adiantum capill

  1. AcEST: BP915252 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000069_C12 486 Adiantum capillus-veneris mRNA. clone: YMU001_000069_C12. BP915252... CL1221Contig1 Show BP915252 Clone id YMU001_000069_C12 Library YMU01 Length 486 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000069_C12. Accession BP915252 Tissue type prothallium Developmental stag... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915252...|Adiantum capillus-veneris mRNA, clone: YMU001_000069_C12. (486 letters) Database: uniprot_sprot.fasta 412,52

  2. AcEST: BP920157 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G01 550 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G01. BP92015...7 CL541Contig1 Show BP920157 Clone id YMU001_000133_G01 Library YMU01 Length 550 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000133_G01. Accession BP920157 Tissue type prothallium Developmental stage...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920157|Adiantum capillus-veneris mRNA,...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920157|Adiantum capillus-veneris

  3. AcEST: BP920159 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G03 420 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G03. BP920159 - Show BP92015...is mRNA. clone: YMU001_000133_G03. Accession BP920159 Tissue type prothallium Developmental stage - Contig I... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92015... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92015

  4. AcEST: BP920156 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_F12 587 Adiantum capillus-veneris mRNA. clone: YMU001_000133_F12. BP92015...6 CL200Contig1 Show BP920156 Clone id YMU001_000133_F12 Library YMU01 Length 587 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000133_F12. Accession BP920156 Tissue type prothallium Developmental stage...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920156...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920156|Adiantum

  5. AcEST: BP920000 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000131_G10 490 Adiantum capillus-veneris mRNA. clone: YMU001_000131_G10. BP920000 - Show BP920000...is mRNA. clone: YMU001_000131_G10. Accession BP920000 Tissue type prothallium Developmental stage - Contig I...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920000...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920000|Adiantum capil

  6. AcEST: BP920127 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_D02 541 Adiantum capillus-veneris mRNA. clone: YMU001_000133_D02. BP92012...7 CL3843Contig1 Show BP920127 Clone id YMU001_000133_D02 Library YMU01 Length 541 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000133_D02. Accession BP920127 Tissue type prothallium Developmental stag...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920127|Adiantum capillus-ve... database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920127|Adian

  7. AcEST: BP920120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_C05 434 Adiantum capillus-veneris mRNA. clone: YMU001_000133_C05. BP920120 - Show BP92012...is mRNA. clone: YMU001_000133_C05. Accession BP920120 Tissue type prothallium Developmental stage - Contig I...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920120|Ad...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920120|

  8. AcEST: BP920125 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_C12 422 Adiantum capillus-veneris mRNA. clone: YMU001_000133_C12. BP920125 - Show BP92012...is mRNA. clone: YMU001_000133_C12. Accession BP920125 Tissue type prothallium Developmental stage - Contig I...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92012...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92012

  9. AcEST: BP918377 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_G07 508 Adiantum capillus-veneris mRNA. clone: YMU001_000112_G07. BP9183...77 CL44Contig1 Show BP918377 Clone id YMU001_000112_G07 Library YMU01 Length 508 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000112_G07. Accession BP918377 Tissue type prothallium Developmental stage ...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918377|Adi

  10. AcEST: BP918324 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_B09 441 Adiantum capillus-veneris mRNA. clone: YMU001_000112_B09. BP918324 - Show BP9183...is mRNA. clone: YMU001_000112_B09. Accession BP918324 Tissue type prothallium Developmental stage - Contig I... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918324|Adiantum capillus-veneris

  11. AcEST: BP918328 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_C02 530 Adiantum capillus-veneris mRNA. clone: YMU001_000112_C02. BP918328 - Show BP9183...is mRNA. clone: YMU001_000112_C02. Accession BP918328 Tissue type prothallium Developmental stage - Contig I...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918328|Adiantum capillus-veneris...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918328|Adian

  12. AcEST: BP918339 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_D02 499 Adiantum capillus-veneris mRNA. clone: YMU001_000112_D02. BP918339 - Show BP9183...is mRNA. clone: YMU001_000112_D02. Accession BP918339 Tissue type prothallium Developmental stage - Contig I...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918339|Adiantum capillus-veneris mRNA, c

  13. AcEST: BP918316 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_B01 479 Adiantum capillus-veneris mRNA. clone: YMU001_000112_B01. BP9183...16 CL1887Contig1 Show BP918316 Clone id YMU001_000112_B01 Library YMU01 Length 479 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000112_B01. Accession BP918316 Tissue type prothallium Developmental stag...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918316|Adia...SI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183

  14. AcEST: BP921830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000154_F08 494 Adiantum capillus-veneris mRNA. clone: YMU001_000154_F08. BP92183...0 CL1991Contig1 Show BP921830 Clone id YMU001_000154_F08 Library YMU01 Length 494 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000154_F08. Accession BP921830 Tissue type prothallium Developmental stag... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92183...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92183

  15. AcEST: BP918392 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000113_A02 381 Adiantum capillus-veneris mRNA. clone: YMU001_000113_A02. BP918392 - Show BP9183...is mRNA. clone: YMU001_000113_A02. Accession BP918392 Tissue type prothallium Developmental stage - Contig I...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918392|Adiantum capillus-veneris ...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918392|...TAKLGPNVSISANARIGPGVRLVGCIILDDVEIKEN 355 Query: 183 KCCDNALYHWMEVL-HWKMRQ---EYRESAIMLPNWE*QYLVKMFLCEDEVVVTSC

  16. AcEST: BP918372 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_G02 505 Adiantum capillus-veneris mRNA. clone: YMU001_000112_G02. BP918372 - Show BP9183...is mRNA. clone: YMU001_000112_G02. Accession BP918372 Tissue type prothallium Developmental stage - Contig I...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918372... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918372|Adia

  17. AcEST: DK954183 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK954183 CL1473Contig1 Show DK954183 Clone id TST39A01NGRL0019_M08 Library TST39 Length ...598 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_M08. 5' end sequence. Accession DK954183...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954183...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954183

  18. AcEST: BP913183 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000027_D11 553 Adiantum capillus-veneris mRNA. clone: YMU001_000027_D11. BP913183 - Show BP913183...is mRNA. clone: YMU001_000027_D11. Accession BP913183 Tissue type prothallium Developmental stage - Contig I...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913183|Adiantum capillus-veneris ...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913183

  19. AcEST: BP917183 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000097_D01 417 Adiantum capillus-veneris mRNA. clone: YMU001_000097_D01. BP917183 - Show BP917183...is mRNA. clone: YMU001_000097_D01. Accession BP917183 Tissue type prothallium Developmental stage - Contig I... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917183...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917183|Adiantum capillus-veneris m

  20. AcEST: BP918319 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_B04 586 Adiantum capillus-veneris mRNA. clone: YMU001_000112_B04. BP918319 - Show BP9183...is mRNA. clone: YMU001_000112_B04. Accession BP918319 Tissue type prothallium Developmental stage - Contig I...ew generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918319|Adiantum...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918319|Adiantu

  1. AcEST: BP918308 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_A05 514 Adiantum capillus-veneris mRNA. clone: YMU001_000112_A05. BP918308 - Show BP9183...is mRNA. clone: YMU001_000112_A05. Accession BP918308 Tissue type prothallium Developmental stage - Contig I...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183

  2. AcEST: DK945183 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK945183 CL1Contig2 Show DK945183 Clone id YMU02A01NGRL0008_I01 Library YMU02 Length 232... Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0008_I01. 5' end sequence. Accession DK945183...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945183|Adiantum capillus-v...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945183

  3. AcEST: BP918373 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000112_G03 522 Adiantum capillus-veneris mRNA. clone: YMU001_000112_G03. BP918373 - Show BP9183...is mRNA. clone: YMU001_000112_G03. Accession BP918373 Tissue type prothallium Developmental stage - Contig I...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9183

  4. AcEST: DK961839 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK961839 CL2471Contig1 Show DK961839 Clone id TST39A01NGRL0011_E04 Library TST39 Length ...509 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_E04. 5' end sequence. Accession DK96183...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961839|Adiantum capillus-vene... (30%), Positives = 33/91 (36%) Frame = -3 Query: 362 GSCSKKLFSIKPRTSINKKQRKTLPIYSNVNSALRPLGSLPPG*KRPGSKFSATSVSDLK 183...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961839|Adi

  5. AcEST: BP919570 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000126_F05 404 Adiantum capillus-veneris mRNA. clone: YMU001_000126_F05. BP91957...0 CL2080Contig1 Show BP919570 Clone id YMU001_000126_F05 Library YMU01 Length 404 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000126_F05. Accession BP919570 Tissue type prothallium Developmental stag...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919570|A... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919570|Adiantum c

  6. AcEST: BP911957 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000011_C07 599 Adiantum capillus-veneris mRNA. clone: YMU001_000011_C07. BP911957... CL144Contig1 Show BP911957 Clone id YMU001_000011_C07 Library YMU01 Length 599 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000011_C07. Accession BP911957 Tissue type prothallium Developmental stage...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911957|Adiantum capillus-ve... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911957|Adiant

  7. AcEST: DK960004 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0006_D14 670 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_D1...4. 5' end sequence. DK960004 CL3069Contig1 Show DK960004 Clone id TST39A01NGRL0006_D14 Library TST39 Length ...670 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0006_D14. 5' end sequence. Accession DK96000...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK96000...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960004|Adiantum capil

  8. AcEST: BP912094 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000015_A09 564 Adiantum capillus-veneris mRNA. clone: YMU001_000015_A09. BP9120...94 CL2967Contig1 Show BP912094 Clone id YMU001_000015_A09 Library YMU01 Length 564 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000015_A09. Accession BP912094 Tissue type prothallium Developmental stag... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912094|Adiantum c...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  9. AcEST: BP912061 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_F01 532 Adiantum capillus-veneris mRNA. clone: YMU001_000012_F01. BP912061 - Show BP9120...is mRNA. clone: YMU001_000012_F01. Accession BP912061 Tissue type prothallium Developmental stage - Contig I...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  10. AcEST: BP912025 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_B09 484 Adiantum capillus-veneris mRNA. clone: YMU001_000012_B09. BP9120...25 CL1441Contig1 Show BP912025 Clone id YMU001_000012_B09 Library YMU01 Length 484 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_B09. Accession BP912025 Tissue type prothallium Developmental stag...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120

  11. AcEST: BP921208 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000147_A03 436 Adiantum capillus-veneris mRNA. clone: YMU001_000147_A03. BP921208 - Show BP92120...is mRNA. clone: YMU001_000147_A03. Accession BP921208 Tissue type prothallium Developmental stage - Contig I..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92120... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921208|Adiantum c

  12. AcEST: DK962120 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK962120 CL775Contig1 Show DK962120 Clone id TST39A01NGRL0013_A06 Library TST39 Length 3...25 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0013_A06. 5' end sequence. Accession DK962120... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962120...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK962120|Adiantum ca

  13. AcEST: BP912073 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000012_G04 498 Adiantum capillus-veneris mRNA. clone: YMU001_000012_G04. BP9120...73 CL1947Contig1 Show BP912073 Clone id YMU001_000012_G04 Library YMU01 Length 498 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000012_G04. Accession BP912073 Tissue type prothallium Developmental stag... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9120...997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2

  14. AcEST: BP915155 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000067_B08 468 Adiantum capillus-veneris mRNA. clone: YMU001_000067_B08. BP91515...5 CL2592Contig1 Show BP915155 Clone id YMU001_000067_B08 Library YMU01 Length 468 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000067_B08. Accession BP915155 Tissue type prothallium Developmental stag... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915155|Adiantum capillus-ven...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915

  15. AcEST: BP921521 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000150_H10 495 Adiantum capillus-veneris mRNA. clone: YMU001_000150_H10. BP921521... CL1722Contig1 Show BP921521 Clone id YMU001_000150_H10 Library YMU01 Length 495 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000150_H10. Accession BP921521 Tissue type prothallium Developmental stag... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921521...n database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921521|Adia

  16. AcEST: BP921219 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000147_B05 600 Adiantum capillus-veneris mRNA. clone: YMU001_000147_B05. BP92121...9 CL3191Contig1 Show BP921219 Clone id YMU001_000147_B05 Library YMU01 Length 600 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000147_B05. Accession BP921219 Tissue type prothallium Developmental stag...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921219|Adiantum capillus-...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92121

  17. AcEST: BP917100 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000095_H06 514 Adiantum capillus-veneris mRNA. clone: YMU001_000095_H06. BP917100 - Show BP917100...is mRNA. clone: YMU001_000095_H06. Accession BP917100 Tissue type prothallium Developmental stage - Contig I...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917100|... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917100

  18. AcEST: DK951001 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK951001 - Show DK951001 Clone id TST38A01NGRL0010_D15 Library TST38 Length 665 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0010_D15. 5' end sequence. Accession DK951001 Tissue t... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK95100...f protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK951001|Adiantum capillus-vener...h = 224 Score = 174 bits (442), Expect = 3e-42 Identities = 100/192 (52%), Positi

  19. AcEST: DK952100 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK952100 - Show DK952100 Clone id TST38A01NGRL0013_C23 Library TST38 Length 631 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_C23. 5' end sequence. Accession DK952100 Tissue t... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952100|Adiantum capillus-ven... PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952100

  20. AcEST: DK952432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK952432 CL2129Contig1 Show DK952432 Clone id TST38A01NGRL0014_B06 Library TST38 Length ...644 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_B06. 5' end sequence. Accession DK952432...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952432|Adi...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952432

  1. AcEST: BP914326 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F07 599 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F07. BP91432...6 CL274Contig1 Show BP914326 Clone id YMU001_000057_F07 Library YMU01 Length 599 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000057_F07. Accession BP914326 Tissue type prothallium Developmental stage...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914326...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432

  2. AcEST: BP914328 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000057_F09 454 Adiantum capillus-veneris mRNA. clone: YMU001_000057_F09. BP914328 - Show BP91432...is mRNA. clone: YMU001_000057_F09. Accession BP914328 Tissue type prothallium Developmental stage - Contig I...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91432

  3. AcEST: DK945432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK945432 CL122Contig1 Show DK945432 Clone id YMU02A01NGRL0009_E18 Library YMU02 Length 2...39 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0009_E18. 5' end sequence. Accession DK945432...BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945432...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK945432|Adiantum capillu

  4. AcEST: DK950432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK950432 CL4Contig1 Show DK950432 Clone id TST38A01NGRL0008_K07 Library TST38 Length 613... Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0008_K07. 5' end sequence. Accession DK950432...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950432|Adiantum capillus-veneris ...AST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950432

  5. AcEST: DK961432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK961432 - Show DK961432 Clone id TST39A01NGRL0010_B17 Library TST39 Length 697 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0010_B17. 5' end sequence. Accession DK961432 Tissue t... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961432|Adian...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961432

  6. AcEST: DK946432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK946432 - Show DK946432 Clone id YMU02A01NGRL0012_J22 Library YMU02 Length 267 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0012_J22. 5' end sequence. Accession DK946432 Tissue t...Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946432...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946432|Adiantum

  7. AcEST: DK959432 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK959432 - Show DK959432 Clone id TST39A01NGRL0004_K19 Library TST39 Length 659 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_K19. 5' end sequence. Accession DK959432 Tissue t...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959432|Adi...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959432|A...H S IF +VG D LY++SEA+DVV+ Sbjct: 374 -KAEVSESPGERSTAQAQSEKGLEIIEVYKSSIHNSAIFASVGEDKGNLYTASEATDVVF 432 Query:

  8. AcEST: BP919384 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000124_E05 553 Adiantum capillus-veneris mRNA. clone: YMU001_000124_E05. BP919384 - Show BP91938...is mRNA. clone: YMU001_000124_E05. Accession BP919384 Tissue type prothallium Developmental stage - Contig I... generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91938...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91938

  9. AcEST: BP919381 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000124_E02 515 Adiantum capillus-veneris mRNA. clone: YMU001_000124_E02. BP919381 - Show BP91938...is mRNA. clone: YMU001_000124_E02. Accession BP919381 Tissue type prothallium Developmental stage - Contig I...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91938...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919381|Adiantum capillus-veneris

  10. AcEST: BP919385 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000124_E07 296 Adiantum capillus-veneris mRNA. clone: YMU001_000124_E07. BP919385 - Show BP91938...is mRNA. clone: YMU001_000124_E07. Accession BP919385 Tissue type prothallium Developmental stage - Contig I...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91938...25:3389-3402. Query= BP919385|Adiantum capillus-veneris mRNA, clone: YMU001_00012...1997), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res.

  11. AcEST: BP920052 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_E03 500 Adiantum capillus-veneris mRNA. clone: YMU001_000132_E03. BP92005...2 CL183Contig1 Show BP920052 Clone id YMU001_000132_E03 Library YMU01 Length 500 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000132_E03. Accession BP920052 Tissue type prothallium Developmental stage...neration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92005...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920052|Adian

  12. AcEST: BP920058 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_E09 507 Adiantum capillus-veneris mRNA. clone: YMU001_000132_E09. BP92005...8 CL621Contig1 Show BP920058 Clone id YMU001_000132_E09 Library YMU01 Length 507 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000132_E09. Accession BP920058 Tissue type prothallium Developmental stage... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92005...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920058|Adiantu

  13. AcEST: BP920051 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000132_E02 361 Adiantum capillus-veneris mRNA. clone: YMU001_000132_E02. BP92005...1 CL2821Contig1 Show BP920051 Clone id YMU001_000132_E02 Library YMU01 Length 361 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000132_E02. Accession BP920051 Tissue type prothallium Developmental stag... new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920051|Adiant... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92005

  14. AcEST: BP919684 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000127_H09 516 Adiantum capillus-veneris mRNA. clone: YMU001_000127_H09. BP919684 - Show BP91968...is mRNA. clone: YMU001_000127_H09. Accession BP919684 Tissue type prothallium Developmental stage - Contig I...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919684...BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91968

  15. AcEST: BP919687 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000128_A01 517 Adiantum capillus-veneris mRNA. clone: YMU001_000128_A01. BP919687 - Show BP91968...is mRNA. clone: YMU001_000128_A01. Accession BP919687 Tissue type prothallium Developmental stage - Contig I...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91968...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91968

  16. AcEST: BP917220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000097_G09 125 Adiantum capillus-veneris mRNA. clone: YMU001_000097_G09. BP917220 - Show BP917220...is mRNA. clone: YMU001_000097_G09. Accession BP917220 Tissue type prothallium Developmental stage - Contig I...T and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917220...rotein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917220|Adiantum capillus-veneris

  17. AcEST: DK946220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK946220 - Show DK946220 Clone id YMU02A01NGRL0011_N24 Library YMU02 Length 518 Definiti...on Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0011_N24. 5' end sequence. Accession DK946220 Tissue t...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946220... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK946220

  18. AcEST: DK959220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 6. 5' end sequence. DK959220 CL2407Contig1 Show DK959220 Clone id TST39A01NGRL0004_B16 Library TST39 Length ...668 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0004_B16. 5' end sequence. Accession DK959220... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959220...on of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK959220

  19. AcEST: BP912220 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000016_E08 357 Adiantum capillus-veneris mRNA. clone: YMU001_000016_E08. BP912220 - Show BP912220...is mRNA. clone: YMU001_000016_E08. Accession BP912220 Tissue type prothallium Developmental stage - Contig I...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912220...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP912220

  20. AcEST: BP919888 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000130_C11 410 Adiantum capillus-veneris mRNA. clone: YMU001_000130_C11. BP919888 - Show BP91988...is mRNA. clone: YMU001_000130_C11. Accession BP919888 Tissue type prothallium Developmental stage - Contig I...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919888|Adiantum capillu...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919888|Adiantum capillus-

  1. AcEST: BP917077 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000095_F02 487 Adiantum capillus-veneris mRNA. clone: YMU001_000095_F02. BP91707...7 CL10Contig1 Show BP917077 Clone id YMU001_000095_F02 Library YMU01 Length 487 Definition Adiantum capi...llus-veneris mRNA. clone: YMU001_000095_F02. Accession BP917077 Tissue type prothallium Developmental stage ... protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91707...AST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917077|

  2. AcEST: BP911616 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000007_B05 303 Adiantum capillus-veneris mRNA. clone: YMU001_000007_B05. BP911616 - Show BP911616...is mRNA. clone: YMU001_000007_B05. Accession BP911616 Tissue type prothallium Developmental stage - Contig I...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911616|Adiantum capillus-... and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911616...132 PE=4 SV=1 Length = 278 Score = 69.7 bits (169), Expect = 7e-11 Identities = 2

  3. AcEST: BP919949 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000131_B01 318 Adiantum capillus-veneris mRNA. clone: YMU001_000131_B01. BP91994...9 CL2311Contig1 Show BP919949 Clone id YMU001_000131_B01 Library YMU01 Length 318 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000131_B01. Accession BP919949 Tissue type prothallium Developmental stag...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91994...a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91994

  4. AcEST: BP918302 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000111_H03 457 Adiantum capillus-veneris mRNA. clone: YMU001_000111_H03. BP918302 - Show BP91830...is mRNA. clone: YMU001_000111_H03. Accession BP918302 Tissue type prothallium Developmental stage - Contig I...ST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91830...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918302|Adiantum ca

  5. AcEST: DK961830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 9. 5' end sequence. DK961830 CL88Contig1 Show DK961830 Clone id TST39A01NGRL0011_D19 Library TST39 Length 68...2 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0011_D19. 5' end sequence. Accession DK961830...database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961830|Adiant...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK961830|Adiantum capillus-veneris m

  6. AcEST: DK947830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK947830 CL1Contig2 Show DK947830 Clone id YMU02A01NGRM0001_E04 Library YMU02 Length 173... Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRM0001_E04. 5' end sequence. Accession DK947830...), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947830...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK947830

  7. AcEST: BP915830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000077_E09 601 Adiantum capillus-veneris mRNA. clone: YMU001_000077_E09. BP915830 - Show BP915830...is mRNA. clone: YMU001_000077_E09. Accession BP915830 Tissue type prothallium Developmental stage - Contig I...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915830|Adiantu...otein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915830|Adiantum capillus-veneris m

  8. AcEST: DK952830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK952830 CL594Contig1 Show DK952830 Clone id TST38A01NGRL0015_C14 Library TST38 Length 6...25 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0015_C14. 5' end sequence. Accession DK952830...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952830|Adiantum ...ST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK952830|A

  9. AcEST: DK950830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK950830 CL851Contig1 Show DK950830 Clone id TST38A01NGRL0009_L23 Library TST38 Length 6...29 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0009_L23. 5' end sequence. Accession DK950830...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950830...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK950830

  10. AcEST: DK948830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK948830 - Show DK948830 Clone id TST38A01NGRL0004_F18 Library TST38 Length 631 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0004_F18. 5' end sequence. Accession DK948830 Tissue t...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948830|Adiantu...tion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948830|Adiantum capillus

  11. AcEST: DK957830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 0. 5' end sequence. DK957830 CL902Contig1 Show DK957830 Clone id TST39A01NGRL0029_G10 Library TST39 Length 5...39 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0029_G10. 5' end sequence. Accession DK957830... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957830...ion of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK957830

  12. AcEST: DK960830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 1. 5' end sequence. DK960830 CL833Contig1 Show DK960830 Clone id TST39A01NGRL0008_H11 Library TST39 Length 5...81 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0008_H11. 5' end sequence. Accession DK960830... database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960830|Adiantum capillus-veneris mRNA, ...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK960830|Adiantu

  13. AcEST: DK943830 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5. 5' end sequence. DK943830 CL194Contig1 Show DK943830 Clone id YMU02A01NGRL0004_B15 Library YMU02 Length 6...87 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_B15. 5' end sequence. Accession DK943830..., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943830...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK943830|Adiantu

  14. AcEST: BP914814 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000063_B08 487 Adiantum capillus-veneris mRNA. clone: YMU001_000063_B08. BP914814 - Show BP914814...is mRNA. clone: YMU001_000063_B08. Accession BP914814 Tissue type prothallium Developmental stage - Contig I...ation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP914814...|Adiantum capillus-veneris mRNA, clone: YMU001_000063_B08. (487 letters) Database: uniprot_sprot.f...-trehalose-phosphate sy... 60 7e-15 sp|O14081|TPSX_SCHPO Putative alpha,alpha-trehalose-phosphate sy... 79 1e-14

  15. AcEST: BP921414 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000149_E11 396 Adiantum capillus-veneris mRNA. clone: YMU001_000149_E11. BP921414 - Show BP921414... Clone id YMU001_000149_E11 Library YMU01 Length 396 Definition Adiantum capillus-vener...is mRNA. clone: YMU001_000149_E11. Accession BP921414 Tissue type prothallium Developmental stage - Contig I... of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP921414|Adiantum capillus-ven...eris mRNA, clone: YMU001_000149_E11. (396 letters) Database: uniprot_sprot.fasta 412,525 sequences; 14

  16. AcEST: BP917075 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000095_E12 529 Adiantum capillus-veneris mRNA. clone: YMU001_000095_E12. BP917075 - Show BP917075...is mRNA. clone: YMU001_000095_E12. Accession BP917075 Tissue type prothallium Developmental stage - Contig I...generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917075...in database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917075|Adiantum capillus-veneris mRNA

  17. AcEST: BP918650 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000116_A01 560 Adiantum capillus-veneris mRNA. clone: YMU001_000116_A01. BP918650... CL552Contig1 Show BP918650 Clone id YMU001_000116_A01 Library YMU01 Length 560 Definition Adiantum cap...illus-veneris mRNA. clone: YMU001_000116_A01. Accession BP918650 Tissue type prothallium Developmental stage...n of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918650|Adiantum capillus-ve...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918650|Adiant

  18. AcEST: BP920169 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_H03 505 Adiantum capillus-veneris mRNA. clone: YMU001_000133_H03. BP920169 - Show BP92016...is mRNA. clone: YMU001_000133_H03. Accession BP920169 Tissue type prothallium Developmental stage - Contig I...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920169|Adiantum cap

  19. AcEST: BP920161 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G06 542 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G06. BP920161 - Show BP92016...is mRNA. clone: YMU001_000133_G06. Accession BP920161 Tissue type prothallium Developmental stage - Contig I...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP9201...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016

  20. AcEST: BP920166 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G12 149 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G12. BP920166 - Show BP92016...is mRNA. clone: YMU001_000133_G12. Accession BP920166 Tissue type prothallium Developmental stage - Contig I...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920166|Adiantum capillus-veneris

  1. AcEST: BP920165 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000133_G11 301 Adiantum capillus-veneris mRNA. clone: YMU001_000133_G11. BP920165 - Show BP92016...is mRNA. clone: YMU001_000133_G11. Accession BP920165 Tissue type prothallium Developmental stage - Contig I...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP920165...and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP92016

  2. AcEST: BP913400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000029_G11 552 Adiantum capillus-veneris mRNA. clone: YMU001_000029_G11. BP913400... CL2174Contig1 Show BP913400 Clone id YMU001_000029_G11 Library YMU01 Length 552 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000029_G11. Accession BP913400 Tissue type prothallium Developmental stag...PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP913400...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25

  3. AcEST: DK954007 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8. 5' end sequence. DK954007 - Show DK954007 Clone id TST39A01NGRL0019_E18 Library TST39 Length 440 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_E18. 5' end sequence. Accession DK954007 Tissue t...LAST: a new generation of protein database search programs, Nucleic Acids Res. 25...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK954007|A...TST39A01NGRL0019_E18 440 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0019_E1

  4. AcEST: DK963400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK963400 CL3471Contig1 Show DK963400 Clone id TST39A01NGRL0016_G24 Library TST39 Length ...625 Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0016_G24. 5' end sequence. Accession DK963400...I-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK963400...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK963400

  5. AcEST: DK955400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 3. 5' end sequence. DK955400 - Show DK955400 Clone id TST39A01NGRL0022_P23 Library TST39 Length 609 Definiti...on Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0022_P23. 5' end sequence. Accession DK955400 Tissue t...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955400|Adiantum cap...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK955400|Adiantum capillus-vene

  6. AcEST: DK944400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4. 5' end sequence. DK944400 CL138Contig1 Show DK944400 Clone id YMU02A01NGRL0005_O24 Library YMU02 Length 3...49 Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0005_O24. 5' end sequence. Accession DK944400...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944400...eneration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK944400|Adiantum cap

  7. AcEST: DK949400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 7. 5' end sequence. DK949400 - Show DK949400 Clone id TST38A01NGRL0005_O07 Library TST38 Length 691 Definiti...on Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0005_O07. 5' end sequence. Accession DK949400 Tissue t...protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK949400|Adiantum capillus-veneris...L+AEPT A+SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFG Sbjct: 400 DLANQVRPLPELVAEPTDAR...97), Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25

  8. AcEST: DK948400 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 2. 5' end sequence. DK948400 CL407Contig1 Show DK948400 Clone id TST38A01NGRL0003_D12 Library TST38 Length 6...81 Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0003_D12. 5' end sequence. Accession DK948400... BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948400...pped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK948400

  9. AcEST: BP915919 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_E03 400 Adiantum capillus-veneris mRNA. clone: YMU001_000079_E03. BP91591...9 CL3486Contig1 Show BP915919 Clone id YMU001_000079_E03 Library YMU01 Length 400 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000079_E03. Accession BP915919 Tissue type prothallium Developmental stag...d BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591...database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915919|Adiant

  10. AcEST: BP915913 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_D08 427 Adiantum capillus-veneris mRNA. clone: YMU001_000079_D08. BP915913 - Show BP91591...is mRNA. clone: YMU001_000079_D08. Accession BP915913 Tissue type prothallium Developmental stage - Contig I...nd PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591...: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91591

  11. AcEST: BP915912 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000079_D07 482 Adiantum capillus-veneris mRNA. clone: YMU001_000079_D07. BP915912 - Show BP91591...is mRNA. clone: YMU001_000079_D07. Accession BP915912 Tissue type prothallium Developmental stage - Contig I...tein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP915912|Adiantum capillus-veneris mR...BLAST: a new generation of protein database search programs, Nucleic Acids Res. 2...5:3389-3402. Query= BP915912|Adiantum capillus-veneris mRNA, clone: YMU001_000079_D07. (482 letters) Databas

  12. AcEST: BP911591 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000006_G06 296 Adiantum capillus-veneris mRNA. clone: YMU001_000006_G06. BP911591 - Show BP911591...is mRNA. clone: YMU001_000006_G06. Accession BP911591 Tissue type prothallium Developmental stage - Contig I...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911591|Adiantum capil...of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP911591|Adiantum capillus-vene

  13. AcEST: BP919213 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000122_E06 293 Adiantum capillus-veneris mRNA. clone: YMU001_000122_E06. BP919213 - Show BP91921...is mRNA. clone: YMU001_000122_E06. Accession BP919213 Tissue type prothallium Developmental stage - Contig I...new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP919213|Adiantu...ped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP91921

  14. AcEST: BP917117 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000096_B11 125 Adiantum capillus-veneris mRNA. clone: YMU001_000096_B11. BP917117... CL2704Contig1 Show BP917117 Clone id YMU001_000096_B11 Library YMU01 Length 125 Definition Adiantum ca...pillus-veneris mRNA. clone: YMU001_000096_B11. Accession BP917117 Tissue type prothallium Developmental stag...T: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917117...w generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP917117

  15. AcEST: BP918100 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YMU001_000109_F01 496 Adiantum capillus-veneris mRNA. clone: YMU001_000109_F01. BP918100... CL8Contig1 Show BP918100 Clone id YMU001_000109_F01 Library YMU01 Length 496 Definition Adiantum capil...lus-veneris mRNA. clone: YMU001_000109_F01. Accession BP918100 Tissue type prothallium Developmental stage -...eration of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918100... a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= BP918100|Adia

  16. EST Express: PHP/MySQL based automated annotation of ESTs from expression libraries

    Directory of Open Access Journals (Sweden)

    Pardinas Jose R

    2008-04-01

    Full Text Available Abstract Background Several biological techniques result in the acquisition of functional sets of cDNAs that must be sequenced and analyzed. The emergence of redundant databases such as UniGene and centralized annotation engines such as Entrez Gene has allowed the development of software that can analyze a great number of sequences in a matter of seconds. Results We have developed "EST Express", a suite of analytical tools that identify and annotate ESTs originating from specific mRNA populations. The software consists of a user-friendly GUI powered by PHP and MySQL that allows for online collaboration between researchers and continuity with UniGene, Entrez Gene and RefSeq. Two key features of the software include a novel, simplified Entrez Gene parser and tools to manage cDNA library sequencing projects. We have tested the software on a large data set (2,016 samples produced by subtractive hybridization. Conclusion EST Express is an open-source, cross-platform web server application that imports sequences from cDNA libraries, such as those generated through subtractive hybridization or yeast two-hybrid screens. It then provides several layers of annotation based on Entrez Gene and RefSeq to allow the user to highlight useful genes and manage cDNA library projects.

  17. PADB : Published Association Database

    Directory of Open Access Journals (Sweden)

    Lee Jin-Sung

    2007-09-01

    Full Text Available Abstract Background Although molecular pathway information and the International HapMap Project data can help biomedical researchers to investigate the aetiology of complex diseases more effectively, such information is missing or insufficient in current genetic association databases. In addition, only a few of the environmental risk factors are included as gene-environment interactions, and the risk measures of associations are not indexed in any association databases. Description We have developed a published association database (PADB; http://www.medclue.com/padb that includes both the genetic associations and the environmental risk factors available in PubMed database. Each genetic risk factor is linked to a molecular pathway database and the HapMap database through human gene symbols identified in the abstracts. And the risk measures such as odds ratios or hazard ratios are extracted automatically from the abstracts when available. Thus, users can review the association data sorted by the risk measures, and genetic associations can be grouped by human genes or molecular pathways. The search results can also be saved to tab-delimited text files for further sorting or analysis. Currently, PADB indexes more than 1,500,000 PubMed abstracts that include 3442 human genes, 461 molecular pathways and about 190,000 risk measures ranging from 0.00001 to 4878.9. Conclusion PADB is a unique online database of published associations that will serve as a novel and powerful resource for reviewing and interpreting huge association data of complex human diseases.

  18. AcEST: DK952058 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 09 sp|Q7ZWU7|EST2B_XENLA Extended synaptotagmin-2-B OS=Xenopus laev... 63 2e-09 sp|Q5FWL4|EST2A_XENLA Extended...=... 62 2e-09 sp|Q3TZZ7|ESYT2_MOUSE Extended synaptotagmin-2 OS=Mus musculus G... 62 3e-09 sp|Q7TNF0|DOC2A_M... OS=Mus musculus... 61 5e-09 sp|A0FGR8|ESYT2_HUMAN Extended synaptotagmin-2 OS=Ho

  19. AcEST: DK946769 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5 sequences; 148,809,765 total letters Searching..................................................done *****... YMU02A01NGRL0013_L01, 5' (132 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  20. AcEST: DK954791 [AcEST

    Lifescience Database Archive (English)

    Full Text Available rs) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX Result : ...ta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  1. AcEST: BP917263 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 8,809,765 total letters Searching..................................................done ***** No hits found ...D11. (179 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  2. AcEST: DK947164 [AcEST

    Lifescience Database Archive (English)

    Full Text Available s) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX Result : T...a 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  3. AcEST: DK947352 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5' (137 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Bla...ot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  4. AcEST: DK945548 [AcEST

    Lifescience Database Archive (English)

    Full Text Available (119 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Blast..._trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  5. AcEST: BP915641 [AcEST

    Lifescience Database Archive (English)

    Full Text Available (325 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (r....fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  6. AcEST: DK947734 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX Result ...asta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  7. AcEST: DK947689 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 525 sequences; 148,809,765 total letters Searching..................................................done ***...e: YMU02A01NGRL0016_L04, 5' (104 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 ...total letters Searching..................................................done ***** No hits found ****** ...

  8. AcEST: BP912156 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ences; 148,809,765 total letters Searching..................................................done ***** No hi...1_000015_G04. (187 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  9. AcEST: DK945438 [AcEST

    Lifescience Database Archive (English)

    Full Text Available , 5' (134 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to B...prot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  10. AcEST: DK947227 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5' (119 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Bl...rot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  11. AcEST: DK947896 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ,525 sequences; 148,809,765 total letters Searching..................................................done **...ne: YMU02A02NGRM0001_C11, 5' (107 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440... total letters Searching..................................................done ***** No hits found ****** ...

  12. AcEST: BP920873 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 412,525 sequences; 148,809,765 total letters Searching..................................................don... clone: YMU001_000142_G05. (306 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  13. AcEST: DK945649 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 29 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX R...embl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  14. AcEST: DK944822 [AcEST

    Lifescience Database Archive (English)

    Full Text Available es; 148,809,765 total letters Searching..................................................done ***** No hits ...NGRL0007_E20, 5' (201 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  15. AcEST: DK945605 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 148,809,765 total letters Searching..................................................done ***** No hits foun...0009_N22, 5' (206 letters) Database: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching.....................................................done ***** No hits found ****** ...

  16. AcEST: BP916808 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ne: YMU001_000091_H03. (159 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (relea...atabase: uniprot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  17. AcEST: DK946471 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 5' (119 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Bla...ot_trembl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  18. AcEST: DK962421 [AcEST

    Lifescience Database Archive (English)

    Full Text Available letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (releas...l.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  19. AcEST: BP913622 [AcEST

    Lifescience Database Archive (English)

    Full Text Available s) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX Result : T...1,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  20. AcEST: BP915145 [AcEST

    Lifescience Database Archive (English)

    Full Text Available (116 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Blast...l.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  1. AcEST: DK944424 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to BlastX Result ...asta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  2. AcEST: BP921273 [AcEST

    Lifescience Database Archive (English)

    Full Text Available . (138 letters) Database: uniprot_sprot.fasta 412,525 sequences; 148,809,765 total letters Searching.....................................................done ***** No hits found ****** TrEMBL (release 39.9) Link to Blas...bl.fasta 7,341,751 sequences; 2,391,615,440 total letters Searching..................................................done ***** No hits found ****** ...

  3. Product Licenses Database Application

    CERN Document Server

    Tonkovikj, Petar

    2016-01-01

    The goal of this project is to organize and centralize the data about software tools available to CERN employees, as well as provide a system that would simplify the license management process by providing information about the available licenses and their expiry dates. The project development process is consisted of two steps: modeling the products (software tools), product licenses, legal agreements and other data related to these entities in a relational database and developing the front-end user interface so that the user can interact with the database. The result is an ASP.NET MVC web application with interactive views for displaying and managing the data in the underlying database.

  4. Plant Genome Duplication Database.

    Science.gov (United States)

    Lee, Tae-Ho; Kim, Junah; Robertson, Jon S; Paterson, Andrew H

    2017-01-01

    Genome duplication, widespread in flowering plants, is a driving force in evolution. Genome alignments between/within genomes facilitate identification of homologous regions and individual genes to investigate evolutionary consequences of genome duplication. PGDD (the Plant Genome Duplication Database), a public web service database, provides intra- or interplant genome alignment information. At present, PGDD contains information for 47 plants whose genome sequences have been released. Here, we describe methods for identification and estimation of dates of genome duplication and speciation by functions of PGDD.The database is freely available at http://chibba.agtec.uga.edu/duplication/.

  5. Phase Equilibria Diagrams Database

    Science.gov (United States)

    SRD 31 NIST/ACerS Phase Equilibria Diagrams Database (PC database for purchase)   The Phase Equilibria Diagrams Database contains commentaries and more than 21,000 diagrams for non-organic systems, including those published in all 21 hard-copy volumes produced as part of the ACerS-NIST Phase Equilibria Diagrams Program (formerly titled Phase Diagrams for Ceramists): Volumes I through XIV (blue books); Annuals 91, 92, 93; High Tc Superconductors I & II; Zirconium & Zirconia Systems; and Electronic Ceramics I. Materials covered include oxides as well as non-oxide systems such as chalcogenides and pnictides, phosphates, salt systems, and mixed systems of these classes.

  6. Database machine performance

    Energy Technology Data Exchange (ETDEWEB)

    Cesarini, F.; Salza, S.

    1987-01-01

    This book is devoted to the important problem of database machine performance evaluation. The book presents several methodological proposals and case studies, that have been developed within an international project supported by the European Economic Community on Database Machine Evaluation Techniques and Tools in the Context of the Real Time Processing. The book gives an overall view of the modeling methodologies and the evaluation strategies that can be adopted to analyze the performance of the database machine. Moreover, it includes interesting case studies and an extensive bibliography.

  7. ALICE Geometry Database

    CERN Document Server

    Santo, J

    1999-01-01

    The ALICE Geometry Database project consists of the development of a set of data structures to store the geometrical information of the ALICE Detector. This Database will be used in Simulation, Reconstruction and Visualisation and will interface with existing CAD systems and Geometrical Modellers.At the present time, we are able to read a complete GEANT3 geometry, to store it in our database and to visualise it. On disk, we store different geometry files in hierarchical fashion, and all the nodes, materials, shapes, configurations and transformations distributed in this tree structure. The present status of the prototype and its future evolution will be presented.

  8. Danish Pancreatic Cancer Database

    DEFF Research Database (Denmark)

    Fristrup, Claus; Detlefsen, Sönke; Palnæs Hansen, Carsten

    2016-01-01

    AIM OF DATABASE: The Danish Pancreatic Cancer Database aims to prospectively register the epidemiology, diagnostic workup, diagnosis, treatment, and outcome of patients with pancreatic cancer in Denmark at an institutional and national level. STUDY POPULATION: Since May 1, 2011, all patients......, and survival. The results are published annually. CONCLUSION: The Danish Pancreatic Cancer Database has registered data on 2,217 patients with microscopically verified ductal adenocarcinoma of the pancreas. The data have been obtained nationwide over a period of 4 years and 2 months. The completeness...

  9. Update History of This Database - Trypanosomes Database | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us Trypanosomes Database Up...date History of This Database Date Update contents 2014/05/07 The contact informatio...p://www.tanpaku.org/tdb/ ) is opened. Joomla SEF URLs by Artio About This Database Database Description Download License Up...date History of This Database Site Policy | Contact Us Update History of This Database - Trypanosomes Database | LSDB Archive ...

  10. 菊芋预处理及菊糖提取工艺优化的研究%Pretreatment from Jerusalem Artichoke and Optimizing of Extraction Techniques on Inulin

    Institute of Scientific and Technical Information of China (English)

    宋含; 王家林

    2013-01-01

    Jerusalem artichoke tubers contain very rich inulin, inulin is an important health functions, in recent years, their effectiveness is perceived by people. This study provides a theoretical basis for industrial production, to seek suitable for industrial production of process conditions. Jerusalem artichoke tubers as raw materials in order to determine the enzyme inactivation blanching 65℃ drying pretreatment; using single factor and orthogonal experi-mental design experimental design methods, extraction temperature, extraction time, solid-liquid ratio, extraction times four factors research. The results showed that: artichoke inulin hot water extraction of optimum extraction conditions were: temperature 60℃, solid-liquid ratio 1∶25, extraction time 60min, extraction times a second.%  菊芋茎块中含有非常丰富菊糖,菊糖是具有重要的保健功能,近年来其功效被人们所认知。本研究为工业化大生产提供理论依据,寻求适合工业化生产的工艺条件。以菊芋茎块为原料,确定了热烫灭酶65℃烘干的预处理工艺;采用单因素试验设计和正交试验设计方法,对提取温度、提取时间、料液比、提取次数4个因素进行研究。结果表明:菊芋菊糖热水浸提的最佳提取工艺条件为:温度60℃、料液比1∶25、浸提时间60min、提取次数为1次。

  11. The Jungle Database Search Engine

    DEFF Research Database (Denmark)

    Bøhlen, Michael Hanspeter; Bukauskas, Linas; Dyreson, Curtis

    1999-01-01

    Information spread in in databases cannot be found by current search engines. A database search engine is capable to access and advertise database on the WWW. Jungle is a database search engine prototype developed at Aalborg University. Operating through JDBC connections to remote databases, Jungle...

  12. Un ami qui est toujours

    Institute of Scientific and Technical Information of China (English)

    王艳

    2012-01-01

    Un jour,pendant la récréation,je suis descendue dans le bureau du secrétariat.Quand je préparais un café,une femme professeur d'espagnol s'est exclamée:《Tiens! Tu es de bonne humeur ce matin ! 》 (E)tonnée,je lui ai demandé pourquoi.Elle a répondu:《 Parce que tu prends un ca fé.》Quelle réponse inattendue! Ce n'est qu'un café instantané,un peu fade,pas très bon,à dire vrai.Si j'en prends une petite tasse,ce n'est pas que je suis de bonne humeur.C'est plut(o)t mon habitude,ou bien c'est que le café me donne une belle humeur.

  13. EST sequencing of Onychophora and phylogenomic analysis of Metazoa.

    Science.gov (United States)

    Roeding, Falko; Hagner-Holler, Silke; Ruhberg, Hilke; Ebersberger, Ingo; von Haeseler, Arndt; Kube, Michael; Reinhardt, Richard; Burmester, Thorsten

    2007-12-01

    Onychophora (velvet worms) represent a small animal taxon considered to be related to Euarthropoda. We have obtained 1873 5' cDNA sequences (expressed sequence tags, ESTs) from the velvet worm Epiperipatus sp., which were assembled into 833 contigs. BLAST similarity searches revealed that 51.9% of the contigs had matches in the protein databases with expectation values lower than 10(-4). Most ESTs had the best hit with proteins from either Chordata or Arthropoda (approximately 40% respectively). The ESTs included sequences of 27 ribosomal proteins. The orthologous sequences from 28 other species of a broad range of phyla were obtained from the databases, including other EST projects. A concatenated amino acid alignment comprising 5021 positions was constructed, which covers 4259 positions when problematic regions were removed. Bayesian and maximum likelihood methods place Epiperipatus within the monophyletic Ecdysozoa (Onychophora, Arthropoda, Tardigrada and Nematoda), but its exact relation to the Euarthropoda remained unresolved. The "Articulata" concept was not supported. Tardigrada and Nematoda formed a well-supported monophylum, suggesting that Tardigrada are actually Cycloneuralia. In agreement with previous studies, we have demonstrated that random sequencing of cDNAs results in sequence information suitable for phylogenomic approaches to resolve metazoan relationships.

  14. Fine Arts Database (FAD)

    Data.gov (United States)

    General Services Administration — The Fine Arts Database records information on federally owned art in the control of the GSA; this includes the location, current condition and information on artists.

  15. National Assessment Database

    Data.gov (United States)

    U.S. Environmental Protection Agency — The National Assessment Database stores and tracks state water quality assessment decisions, Total Maximum Daily Loads (TMDLs) and other watershed plans designed to...

  16. Disaster Debris Recovery Database

    Data.gov (United States)

    U.S. Environmental Protection Agency — The US EPA Region 5 Disaster Debris Recovery Database includes public datasets of over 3,500 composting facilities, demolition contractors, haulers, transfer...

  17. Consumer Product Category Database

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Chemical and Product Categories database (CPCat) catalogs the use of over 40,000 chemicals and their presence in different consumer products. The chemical use...

  18. Molecular marker databases.

    Science.gov (United States)

    Lai, Kaitao; Lorenc, Michał Tadeusz; Edwards, David

    2015-01-01

    The detection and analysis of genetic variation plays an important role in plant breeding and this role is increasing with the continued development of genome sequencing technologies. Molecular genetic markers are important tools to characterize genetic variation and assist with genomic breeding. Processing and storing the growing abundance of molecular marker data being produced requires the development of specific bioinformatics tools and advanced databases. Molecular marker databases range from species specific through to organism wide and often host a variety of additional related genetic, genomic, or phenotypic information. In this chapter, we will present some of the features of plant molecular genetic marker databases, highlight the various types of marker resources, and predict the potential future direction of crop marker databases.

  19. Reach Address Database (RAD)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Reach Address Database (RAD) stores the reach address of each Water Program feature that has been linked to the underlying surface water features (streams,...

  20. National Geochemical Database: Sediment

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Geochemical analysis of sediment samples from the National Geochemical Database. Primarily inorganic elemental concentrations, most samples are of stream sediment...