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Sample records for aromatica str rcb

  1. Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation

    Energy Technology Data Exchange (ETDEWEB)

    Salinero, Kennan Kellaris; Keller, Keith; Feil, William S.; Feil, Helene; Trong, Stephan; Di Bartolo, Genevieve; Lapidus, Alla

    2008-11-17

    Initial interest in Dechloromonas aromatica strain RCB arose from its ability to anaerobically degrade benzene. It is also able to reduce perchlorate and oxidize chlorobenzoate, toluene, and xylene, creating interest in using this organism for bioremediation. Little physiological data has been published for this microbe. It is considered to be a free-living organism. The a priori prediction that the D. aromatica genome would contain previously characterized 'central' enzymes involved in anaerobic aromatic degradation proved to be false, suggesting the presence of novel anaerobic aromatic degradation pathways in this species. These missing pathways include the benzyl succinyl synthase (bssABC) genes (responsible for formate addition to toluene) and the central benzoylCoA pathway for monoaromatics. In depth analyses using existing TIGRfam, COG, and InterPro models, and the creation of de novo HMM models, indicate a highly complex lifestyle with a large number of environmental sensors and signaling pathways, including a relatively large number of GGDEF domain signal receptors and multiple quorum sensors. A number of proteins indicate interactions with an as yet unknown host, as indicated by the presence of predicted cell host remodeling enzymes, effector enzymes, hemolysin-like proteins, adhesins, NO reductase, and both type III and type VI secretory complexes. Evidence of biofilm formation including a proposed exopolysaccharide complex with the somewhat rare exosortase (epsH), is also present. Annotation described in this paper also reveals evidence for several metabolic pathways that have yet to be observed experimentally, including a sulphur oxidation (soxFCDYZAXB) gene cluster, Calvin cycle enzymes, and nitrogen fixation (including RubisCo, ribulose-phosphate 3-epimerase, and nif gene families, respectively). Analysis of the D. aromatica genome indicates there is much to be learned regarding the metabolic capabilities, and life-style, for this microbial

  2. ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF CURCUMA AROMATICA

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    Ammayappan Rajam Srividya

    2012-06-01

    Full Text Available The objective of this paper is to find out the antidiabetic activity of Curcuma aromatica. In this research paper we dealt with antioxidant activities by DPPH method, ABTS method, Lipid peroxidation assay and scavenging ability of the extract for the hydrogen peroxide radical, Glucose uptake by rat hemi diaphragm method. Antidiabetic activity using healthy adult Wister rats were also carried out. Toluene extract of Curcuma aromatica showed the potent scavenging activity by DPPH method with the IC 50 value of 50.62±0.998 µg/ml, by lipid per oxidation method with the IC 50 value of 75±0.87 µg/ml, hydrogen peroxide radical scavenging activity with the IC 50 value 43.75±1.24 µg/ml, and ABTS radical scavenging method with the IC 50 value 0.038±1.54 µg/ml. After the treatment with the toluene extract of Curcuma aromatica, serum glucose level was found to be decreased from 278.53 to 116.5 mg/dl, total protein level increased from 3.09 to 5.78 mg/dl. There was a decrease in total cholesterol level from 292.33 to 134.50 mg/dl, decrease in serum triglyceride level from 85.66 to 64.16mg/dl when compared to diabetic control group. Toluene extract of Curcuma aromatica exhibited significant antioxidant and antidiabetic activities in both in vitro and in vivo models. So, it can be used as alternative herbal medicine in the treatment of diabetes and diabetic induced complication.

  3. Synthesis, Structural Characterization, and Bioactivity of the Stable Peptide RCB-1 from Ricinus communis.

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    Boldbaatar, Delgerbat; Gunasekera, Sunithi; El-Seedi, Hesham R; Göransson, Ulf

    2015-11-25

    The Ricinus communis biomarker peptides RCB-1 to -3 comprise homologous sequences of 19 (RCB-1) or 18 (RCB-2 and -3) amino acid residues. They all include four cysteine moieties, which form two disulfide bonds. However, neither the 3D structure nor the biological activity of any of these peptides is known. The synthesis of RCB-1, using microwave-assisted, Fmoc-based solid-phase peptide synthesis, and a method for its oxidative folding are reported. The tertiary structure of RCB-1, subsequently established using solution-state NMR, reveals a twisted loop fold with antiparallel β-sheets reinforced by the two disulfide bonds. Moreover, RCB-1 was tested for antibacterial, antifungal, and cytotoxic activity, as well as in a serum stability assay, in which it proved to be remarkably stable.

  4. A search for RCB stars in globular clusters

    CERN Document Server

    Boiardi, Scarlett-Rose; Davoust, Emmanuel

    2012-01-01

    There are only about 65 R Coronae Borealis stars known in our Galaxy, and none in globular clusters. As these stars are thought to result from the merger of two white dwarfs, one would expect the higher stellar density of globular clusters to favor their formation. We have searched for such stars in Galactic globular clusters, as their presence in a specific category of clusters might provide more clues as to their formation. We selected from the WISE all-Sky source catalog all the stars within the tidal radius of the 150 globular clusters within 50 kpc, which is the distance to which RCB stars are detectable by WISE. The total number of stars selected in this way was 635989. We then successively applied the eight selection criteria of Tisserand (2012) satisfied by RCB stars to the dereddened photometric WISE and 2MASS data. Only three stars satisfying the conditions were found in the field of three globular clusters. The star in the field of Liller 1 is most probably a protostar. For the two other candidates...

  5. Pathological study of breast cancer by method postneaadyuvancia RCB (residual tumor burden) associated with cell proliferation index report preliminary data

    International Nuclear Information System (INIS)

    Full text: Introduction: The evaluation of residual disease increases prognostic information postneaadyuvancia and obtained by the study of pathological response. Using method index residual tumor burden (RCB)developed at M D Anderson where from morphological parameters (Size of the tumor bed, residual percentage of invasive carcinoma, carcinoma in situ percentage residual metastatic nodes number and size of largest metastasis)the index is calculated. Proliferation index represents an independent predictor of response to particular drugs. A high rate of cell proliferation after chemotherapy is linked with a poor survival We compared the results of both indices Material and Methods: We applied method residual tumor burden index (RCB)in 30 patients operated breast carcinoma after neoadjuvant therapy. Documentation was performed digital macroscopic bed residual tumor, printing and bulk sampling mapping performed on serially reading sheets histological double-blind by two pathologists using graphical illustrations of the percentage of cellularity neoplastic. The application of the formula classified as: RCB 0, complete pathological response (Rpc), RCB I, minimal residual disease, moderate residual disease RCB II, III RBC extensive disease residual. We immunohistochemical proliferation index (PI)with K i 67 in 14 cases (RCB II and III) with double-blinded histological evaluation by performing a percentage of stained nuclei in the greater staining sector thereof and with a cutoff of 14% of stained nuclei. Results: The size of the residual tumor bed was between 4x3mm and 110x60mm. Percentages cellularity invasive component between 0 and 86%, carcinoma in situ between 0 and 30 %. RCB case 0, RCB I a case, RCB RCB II and III seventeen cases eleven cases. Proliferation index was between 1% to 90 %, greater than 14 % in 29% of III and 21% RCB RCB II. Less than 14% was seen in 29% of RCB II and 7% RCB III In six cases there was variation in the rate of pre and post neoadjuvant

  6. All known hot RCB stars are fading fast over the last century

    Science.gov (United States)

    Schaefer, Bradley E.

    2016-08-01

    The R Coronae Borealis (RCB) stars are cool supergiants that display irregular and deep dips in their light curves, caused by dust formation. There are four known hot RCB stars (DY Cen, MV Sgr, V348 Sgr, and HV 2671), with surface temperatures of 15 000-25 000 K, and prior work has suggested that three of these have secular fading in brightness. I have tested this result by measuring century-long light curves in the Johnson B band with modern comparison star magnitudes, and I have extended this by measuring many magnitudes over a wide time range as well as for the fourth hot RCB star. In all four cases, the B band magnitude of the maximum light is now fast fading. The fading rates (in units of magnitudes per century) are 2.5 for DY Cen after 1960, 1.3 for MV Sgr, 1.3 for V348 Sgr, and 0.7 for HV 2671. This secular fading is caused by the expected evolution of the star across the top of the Hertzsprung-Russell (HR) diagram at constant luminosity, as the temperature rises and the bolometric correction changes. For DY Cen, the brightness at maximum light is rising from 1906 to 1932, and this is caused by the temperature increase from near 5800 to 7500 K. Before 1934, DY Cen had frequent dust dips, while after 1934 there are zero dust dips, so there is some apparent connection between the rising temperature and the formation of the dust. Thus, we have watched DY Cen evolve from an ordinary RCB star up to a hot RCB star and now appearing as an extreme helium star, all in under one century.

  7. The RCB star V854 Cen is surrounded by a hot dusty shell

    OpenAIRE

    Chesneau, Olivier; Millour, Florentin; De Marco, Orsola; Bright, S.N.; Spang, Alain; Lagadec, Eric; Mékarnia, Djamel; de Wit, W. J.

    2014-01-01

    Accepted by A&A, new version after language editing International audience Aims : The hydrogen-deficient supergiants known as R Coronae Borealis (RCB) stars might be the result of a double-degenerate merger of two white dwarfs (WDs), or a final helium shell flash in a planetary nebula central star. In this context, any information on the geometry of their circumstellar environment and, in particular, the potential detection of elongated structures, is of great importance. Methods : We o...

  8. All Known Hot RCB Stars Are Fading Fast Over the Last Century

    CERN Document Server

    Schaefer, Bradley E

    2016-01-01

    The R Coronae Borealis (RCB) stars are cool supergiants that display irregular and deep dips in their light curves, caused by dust formation. There are four known hot RCB stars (DY Cen, MV Sgr, V348 Sgr, and HV 2671), with surface temperatures of 15,000--25,000 K, and prior work has suggested that three of these have secular fading in brightness. I have tested this result by measuring century-long light curves in the Johnson B-band with modern comparison star magnitudes, and I have extended this by measuring many magnitudes over a wide time range as well as for the fourth hot RCB star. In all four cases, the B-band magnitude of the maximum light is now fast fading. The fading rates (in units of magnitudes per century) are 2.5 for DY Cen after 1960, 1.3 for MV Sgr, 1.3 for V348 Sgr, and 0.7 for HV 2671. This secular fading is caused by the expected evolution of the star across the top of the HR diagram at constant luminosity, as the temperature rises and the bolometric correction changes. For DY Cen, the brigh...

  9. Alternative energy production pathways in Taenia crassiceps cysticerci in vitro exposed to a benzimidazole derivative (RCB20).

    Science.gov (United States)

    Fraga, Carolina Miguel; Da Costa, Tatiane Luiza; De Castro, Ana Maria; Reynoso-Ducoing, Olivia; Ambrosio, Javier; Hernández-Campos, Alicia; Castillo, Rafael; Vinaud, Marina Clare

    2016-04-01

    Biochemical studies of benzimidazole derivatives are important to determine their mode of action and activity against parasites. The lack of antihelminthic alternatives to treat parasitic infections and albendazole resistance cases make the search for new antiparasitary drugs of utmost importance. The 6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative with promising effect. This study evaluated the effect of different concentrations of RCB20 in the alternative energetic pathway of in vitro Taenia crassiceps cysticerci. The parasites were in vitro exposed to 6.5 and 13 µM of RCB20 and albendazole sulfoxide (ABZSO). The quantification of acetate, acetoacetate, β-hydroxybutyrate, fumarate and propionate was performed by high-performance liquid chromatography. The quantification of urea, creatinine and total proteins was performed by spectrophotometry. The increase in β-hydroxybutyrate reflects the enhancement of the fatty acid oxidation in the treated groups. Volatile fatty acids secretion, acetate and propionate, was increased in the treated groups. The secretion mechanisms of the treated parasites were impaired due to organic acids increased concentrations in the cysticerci. It is possible to conclude that the metabolic effect on alternative energetic pathways is slightly increased in the parasites treated with RCB20 than the ones treated with ABZSO.

  10. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

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    Quy Diem Do

    2014-09-01

    Full Text Available Limnophila aromatica is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100% of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatica extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatica and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatica. The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.

  11. In vitro propagation of Homalomena aromatica Schott., an endangered aromatic medicinal herb of Northeast India

    OpenAIRE

    Raomai, Shiveirou; Kumaria, Suman; Tandon, Pramod

    2013-01-01

    A successful report on the in vitro propagation of Homalomena aromatica via rhizome axillary bud multiplication is presented. Rhizome bud explants were cultured on Murashige and Skoog medium supplemented with various concentrations of cytokinins to induce multiple shoot formation for micropropagation. The highest number of shoots was achieved in MS medium supplemented with 2.0 mg l−1 6-benzylaminopurine. The regenerated shoots rooted most efficiently on half-strength MS medium supplemented wi...

  12. Antitussive activity of ethanolic extract of Curcuma aromatica rhizomes on sulfur dioxide induced cough in mice

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    Marina, G.D.; Kekuda, T.R Prashith; Sudarshan, S.J

    2008-01-01

    Ethanolic extract of rhizomes of Curcuma aromatica (Zingiberaceae) was investigated for its antitussive effect on Sulfur dioxide induced cough model in mice. The extract exhibited significant antitussive activity in a dose dependant manner. The activity was compared with the prototype antitussive agent codeine phosphate. The ethanolic extract at the dose of lOOmg. 200mg and 400mg/kg body weight, po, showed 68%, 74% and 79% of inhibition of cough with respect to control group. PMID:22557276

  13. Mites associated to Xylopia aromatica (Lam. Mart. (Annonaceae in urban and rural fragments of semidecidual forest

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    Felipe M. Nuvoloni

    2011-12-01

    Full Text Available Mites associated to Xylopia aromatica (Lam. Mart. (Annonaceae in urban and rural fragments of semidecidual forest. Native plants can shelter a great diversity of mites. Notwithstanding, the conservation of the forest fragments where the plants are located can influence the structure of the mites community. Generally, in homogenous environments the diversity is lower due to the dominance of one or a few species. In this work, we studied the mite community on Xylopia aromatica (Lam. Mart. (Annonaceae in two fragments of semidecidual forest: one on rural and other on urban area. Seven individuals of X. aromatica were monthly sampled from April 2007 to March 2008, in each of these fragments. Descriptive indexes of diversity, dominance and evenness were applied to verify the ecological patterns of the mite community, besides the Student's t-test to compare the abundance between the fragments. We collected 27,365 mites of 37 species belonging to 11 families. Calacarus sp. (Eriophyidae was the most abundant species, representing 73% of the total sampled. The abundance was greater in the urban fragment (67.7%, with the diversity index reaching only 25% of the theoretical maximum expected. Probably, these values might have been influenced by the location of this fragment in the urban area, being more homogeneous and submitted directly to the presence of atmospheric pollution. In this manner, X. aromatica is able to shelter a higher diversity of mites when inserted in preserved ecosystems, since the highest diversity of available resources allows the establishment of richer and most diverse mite community.

  14. Serum metabonomic analysis of protective effects of Curcuma aromatica oil on renal fibrosis rats.

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    Liangcai Zhao

    Full Text Available BACKGROUND: Curcuma aromatica oil is a traditional herbal medicine demonstrating protective and anti-fibrosis activities in renal fibrosis patients. However, study of its mechanism of action is challenged by its multiple components and multiple targets that its active agent acts on. METHODOLOGY/PRINCIPAL FINDINGS: Nuclear magnetic resonance (NMR-based metabonomics combined with clinical chemistry and histopathology examination were performed to evaluate intervening effects of Curcuma aromatica oil on renal interstitial fibrosis rats induced by unilateral ureteral obstruction. The metabolite levels were compared based on integral values of serum 1H NMR spectra from rats on 3, 7, 14, and 28 days after the medicine administration. Time trajectory analysis demonstrated that metabolic profiles of the agent-treated rats were restored to control levels after 7 days of dosage. The results confirmed that the agent would be an effective anti-fibrosis medicine in a time-dependent manner, especially in early renal fibrosis stage. Targeted metabolite analysis showed that the medicine could lower levels of lipid, acetoacetate, glucose, phosphorylcholine/choline, trimethylamine oxide and raise levels of pyruvate, glycine in the serum of the rats. Serum clinical chemistry and kidney histopathology examination dovetailed well with the metabonomics data. CONCLUSIONS/SIGNIFICANCES: The results substantiated that Curcuma aromatica oil administration can ameliorate renal fibrosis symptoms by inhibiting some metabolic pathways, including lipids metabolism, glycolysis and methylamine metabolism, which are dominating targets of the agent working in vivo. This study further strengthens the novel analytical approach for evaluating the effect of traditional herbal medicine and elucidating its molecular mechanism.

  15. Curcuma aromatica Water Extract Attenuates Ethanol-Induced Gastritis via Enhancement of Antioxidant Status

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    Woo-Young Jeon

    2015-01-01

    Full Text Available Curcuma aromatica is an herbal medicine and traditionally used for the treatment of various diseases in Asia. We investigated the effects of C. aromatica water extract (CAW in the stomach of rats with ethanol-induced gastritis. Gastritis was induced in rats by intragastric administration of 5 mL/kg body weight of absolute ethanol. The CAW groups were given 250 or 500 mg of extract/kg 2 h before administration of ethanol, respectively. To determine the antioxidant effects of CAW, we determined the level of lipid peroxidation, the level of reduced glutathione (GSH, the activities of catalase, degree of inflammation, and mucus production in the stomach. CAW reduced ethanol-induced inflammation and loss of epithelial cells and increased the mucus production in the stomach. CAW reduced the increase in lipid peroxidation associated with ethanol-induced gastritis (250 and 500 mg/kg, p<0.01, resp. and increased mucosal GSH content (500 mg/kg, p<0.01 and the activity of catalase (250 and 500 mg/kg, p<0.01, resp.. CAW increased the production of prostaglandin E2. These findings suggest that CAW protects against ethanol-induced gastric mucosa injury by increasing antioxidant status. We suggest that CAW could be developed for the treatment of gastritis induced by alcohol.

  16. BUS 501 Str course tutorial / uophelp

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    uophel

    2015-01-01

    For more course tutorials visit www.uophelp.com     BUS 501 Week 3 Assignment 1 The Independent Government Cost Estimate and the Statement of Work (Str Course) BUS 501 Week 4 Assignment 2 Types of Contracts and Performance-Based Acquisition (Str Course) BUS 501 Week 7 Assignment 3 Small Business Program and Source Selection Plans (Str Course) BUS 501 Assignment 4 Technical and Cost or Price Evaluations and Price Reasonableness (Str Course) BUS 501 Wee...

  17. Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition

    OpenAIRE

    Duldhardt, Ilka; Gaebel, Julia; Chrzanowski, Lukasz; Nijenhuis, Ivonne; Härtig, Claus; Schauer, Frieder; Heipieper, Hermann J.

    2010-01-01

    Summary The effect of different solvents and pollutants on the cellular fatty acid composition of three bacterial strains: Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans, representatives of diverse predominant anaerobic metabolisms was investigated. As the prevailing adaptive mechanism in cells of T. aromatica and G. sulfurreducens whose cellular fatty acids patterns were dominated by palmitic acid (C16:0) and palmitoleic acid (C16:1cis), the cells reacted by an inc...

  18. Color improvement by irradiation of Curcuma aromatica extract for industrial application

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jae Kyung [Radiation Food Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup 580-185 (Korea, Republic of); Jo, Cheorun [Radiation Food Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup 580-185 (Korea, Republic of); Hwang, Han Joon [Graduate School of Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of); Park, Hyun Jin [Graduate School of Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-Gu, Seoul 136-701 (Korea, Republic of); Kim, Young Ji [Division of Food Beverage and Culinary Arts, Younganm College of Science and Technology, Daegu 705-703 (Korea, Republic of); Byun, Myung Woo [Radiation Food Science and Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup 580-185 (Korea, Republic of)]. E-mail: mwbyun@kaeri.re.kr

    2006-03-15

    Curcuma species are medicinal herbs with various pharmacological activities. They have a characteristic yellow color and contain curcuminoids which are natural antioxidants. In this study, Curcuma aromatica (Canada) and Curcuma longa (CL) extracts were gamma-irradiated for improving the color, and the irradiation effects on the curcuminoids contents in CA and CL extracts were determined in order to evaluate if CA can replace CL on the market, where the price of CA is 70% lower than the price of CL. The Hunter color L*-values were increased significantly in all the samples with increasing dose, while the a*-values and b*-values decreased, which implies that the color of the CA and CL extracts changed from dark yellow to brighter yellow. Curcuminoids contents of all the samples were evaluated, and CA contains more curcuminoids than CL. These results indicated that irradiation improved the properties of CA for possible industrial use in manufacturing food and cosmetic industrial products.

  19. Color improvement by irradiation of Curcuma aromatica extract for industrial application

    Science.gov (United States)

    Kim, Jae Kyung; Jo, Cheorun; Hwang, Han Joon; Park, Hyun Jin; Kim, Young Ji; Byun, Myung Woo

    2006-03-01

    Curcuma species are medicinal herbs with various pharmacological activities. They have a characteristic yellow color and contain curcuminoids which are natural antioxidants. In this study, Curcuma aromatica (CA) and Curcuma longa (CL) extracts were gamma-irradiated for improving the color, and the irradiation effects on the curcuminoids contents in CA and CL extracts were determined in order to evaluate if CA can replace CL on the market, where the price of CA is 70% lower than the price of CL. The Hunter color L*-values were increased significantly in all the samples with increasing dose, while the a*-values and b*-values decreased, which implies that the color of the CA and CL extracts changed from dark yellow to brighter yellow. Curcuminoids contents of all the samples were evaluated, and CA contains more curcuminoids than CL. These results indicated that irradiation improved the properties of CA for possible industrial use in manufacturing food and cosmetic industrial products.

  20. Antibacterial activity of rhizome of curcuma aromatica and partial purification of active compounds

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    S Revathi

    2013-01-01

    Full Text Available The hexane extract of Curcuma aromatica, a plant belonging to the family Zingiberaceae was tested on 10 bacterial strains (clinical isolates and standard strains. Agar diffusion method was adopted for determining the antibacterial activity of the extract. The hexane extract was found to be active against all Gram-positive strains tested, but inactive against Gram-negative strains. The minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 ΅g/ml. The phytochemical analysis of hexane extract by gas chromatography mass spectrometry revealed the presence of 13 compounds. The crude hexane extract was partially purified by thin layer chromatography. The zone showing good antibacterial activity was analysed further by gas chromatography mass spectrometry, UV/Vis spectrophotometry and Fourier transform infrared spectroscopy, which indicated the probable presence of germacrone.

  1. Color improvement by irradiation of Curcuma aromatica extract for industrial application

    International Nuclear Information System (INIS)

    Curcuma species are medicinal herbs with various pharmacological activities. They have a characteristic yellow color and contain curcuminoids which are natural antioxidants. In this study, Curcuma aromatica (Canada) and Curcuma longa (CL) extracts were gamma-irradiated for improving the color, and the irradiation effects on the curcuminoids contents in CA and CL extracts were determined in order to evaluate if CA can replace CL on the market, where the price of CA is 70% lower than the price of CL. The Hunter color L*-values were increased significantly in all the samples with increasing dose, while the a*-values and b*-values decreased, which implies that the color of the CA and CL extracts changed from dark yellow to brighter yellow. Curcuminoids contents of all the samples were evaluated, and CA contains more curcuminoids than CL. These results indicated that irradiation improved the properties of CA for possible industrial use in manufacturing food and cosmetic industrial products

  2. Davis Strædet

    DEFF Research Database (Denmark)

    Råstofdirektoratet planlægger at udbyde flere licensområder med henblik på efterforskning og udvinding af olie og gas i den grønlandske del af Davis Stræde. Som en del af beslutningsgrundlaget har Råstofdirektoratet bedt DCE – Nationalt Center for Miljø og Energi og Grønlands Naturinstitut om at ...

  3. Mutation Rates of STR Systems in Danes

    DEFF Research Database (Denmark)

    Andersen, Kim Emil; Bøttcher, Susanne Gammelgaard; Christensen, Susanne;

    Danish paternity cases in the period 1999 to 2005 were investigated regarding mutation rates in STR loci. STR-typing was performed by the Applied Biosystems AmplfStr Profiler Plus kit in the period 1999 to early 2005, hereafter named the PP9, and by Applied Biosystems AmplfStr Identifier kit...... for the rest of 2005, hereafter named the IDFL. All cases with one to four genetic inconsistencies were manually inspected by two forensic geneticists and statistically analyzed by five statisticians. We found no significant effect of kits and no interaction of kits and STRA loci, but differences in mutation...... rates on different STR loci. In the cases where mutations had occured, we found no interaction between kits, STRA loci or sexes. However, we found differences in the mutation rates between the sexes, meaning that the differences in male and female mutation rates can be assumed constant over STR loci...

  4. Effect of Cinnamomum burmannii Nees ex Bl. and Massoia aromatica Becc. Essential Oils on Planktonic Growth and Biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus In Vitro

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    Sylvia Utami Tunjung Pratiwi

    2015-03-01

    Full Text Available Summary. Biofilms are communities of microorganisms that can be found in almost every habitat. They can be attached to a surface and protected by an extracellular matrix of biomolecules that substantially protect microorganisms from environmental effects. The aim of this research is to explore the potency of essential oils from Cinnamomum burmannii Nees ex Bl. and Massoia aromatica Becc. against planktonic growth and biofilm formation of, two opportunistic pathogens, Pseudomonas aeruginosa PAO1 and Staphylococcus aureus Cowan I. Essential oil from C. burmannii  and M. aromatica showed a 50% inhibition of  P. aeruginosa and S. aureus planktonic growth (PMIC50 at concentration of 0.12 % v/v. Essential oil from C. burmannii and M.  aromatica showed capability to inhibit 50% (MBIC50 of P. aeruginosa and S. aureus biofilm formation at concentration of 0.03 % v/v, whereas higher concentration (0.12 % v/v was needed by C. burmannii and M. aromatica oil to disrupt 50% of P. aeruginosa and S. aureus established biofilm. The analysis by GC-MS showed cinnamic aldehyde (92.02 % to be the major component of C. burmannii essential oil, whereas Massoialactone (92.05 % was the main constituent of M. aromatica essential oil. The results obtained in this study have made the oil of C. burmannii and M. aromatica oil as an interesting source for antibiofilm agents in the development of new strategies to treat infections caused by P. aeruginosa and  S. aureus biofilm.Industrial Relevance. Instead of freely swimming in solution (planktonic, in nature microbial tends to adhere to surfaces, and develop microbial biofilms. Microbial biofilms are exhibits resistance to both antimicrobial drugs and the host defence systems, which often results in persistent and difficult-to-treat infections. This makes the discovery of anti-infective agents which are active against planktonic and biofilm microbial represents an important goal. Plant is an interesting source for finding

  5. Factors affecting the accumulation of curcumin in microrhizomes of Curcuma aromatica Salisb.

    Science.gov (United States)

    Wu, Ke; Zhang, Xiaoxia; Sun, Shulan; Wang, Xiaojing

    2015-01-01

    Curcuminoids, and mainly curcumin, are potential therapeutic agents for the prevention of various diseases; however, little is known about the factors that influence their accumulation in Curcuma species. In this study, the effects of factors such as sucrose concentration, different ratios of 6-benzylaminopurine (6-BA) and α-naphthalene acetic acid (NAA), and light quality on the accumulation of curcumin and other curcuminoids in Curcuma aromatica were investigated. Microrhizomes grown on media containing 3% sucrose produced more curcumin and other curcuminoids than those grown on higher concentrations. Moreover, when compared to other ratios of 6-BA and NAA, microrhizomes induced on 3% sucrose media supplemented with 3.0 mg/L 6-BA and 0.5 mg/L NAA produced more curcumin and other curcuminoids; however, the amount was less than in microrhizomes grown on 3% sucrose alone. We determined that a 5% sucrose medium supplemented with 3.0 mg/L of 6-BA and 0.5 mg/L of NAA enhanced the levels of curcumin and curcuminoids and that exposure to red light further increased production.

  6. Cell proliferative effect of polyxyloses extracted from the rhizomes of wild turmeric, Curcuma aromatica.

    Science.gov (United States)

    Niyomploy, Ploypat; Thunyakitpisal, Pasutha; Karnchanatat, Aphichart; Sangvanich, Polkit

    2010-08-01

    Hot water-soluble crude polysaccharides were extracted from the rhizomes of wild turmeric, Curcuma aromatica Salisb. (Zingiberaceae), using dry grinding, boiling water extraction, and then ethanol precipitation. The crude polysaccharide extract was then fractionated by DEAE-cellulose ion exchange column chromatography, and subsequently further purified by Superdex G-200 gel filtration column chromatography, giving two relatively abundant polysaccharide fractions, called P11 and P21, and a much less common fraction P22 obtained in insufficient amounts for further analysis. The two main polysaccharide fractions were evaluated for monosaccharide composition by acid hydrolysis and high performance liquid chromatography (HPLC), whilst the molecular weight and functional groups were determined by gel permeable chromatography (GPC) and FT-IR, respectively. Fractions P11 and P21 were found to be polyxyloses with molecular weight-averages of 469,171 and 157,665 Da, respectively. P11 (100 microg/mL) could significantly induce human gingival fibroblast cells proliferation by 30%, while P21 (100 microg/mL) could significantly inhibit gingival fibroblast cells proliferation by 92%. The in vitro human primary gingival fibroblast cell proliferation in cell culture at a concentration of 100 microg/mL.

  7. First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica

    Directory of Open Access Journals (Sweden)

    Allah Bakhsh

    2016-08-01

    Full Text Available The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica. A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS and neomycin phosphotransferase II (nptII gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope.

  8. Venezenin: a new bioactive Annonaceous acetogenin from the bark of Xylopia aromatica.

    Science.gov (United States)

    Colman-Saizarbitoria, T; Gu, Z M; Zhao, G X; Zeng, L; Kozlowski, J F; McLaughlin, J L

    1995-04-01

    Asimicin and a new cytotoxic Annonaceous acetogenin, venezenin [1], were isolated from the bark of Xylopia aromatica by bioactivity-directed fractionation using lethality to brine shrimp. Compound 1 represents an unusual type of C37 Annonaceous acetogenin, lacking either tetrahydrofuran (THF) or epoxide rings and possessing a double bond located two methylenes away from a vicinal diol in the hydrocarbon chain. The structure of 1 was elucidated by 1H- and 13C-nmr, COSY, single-relayed COSY, and by HMBC techniques, and derivatization. Annomontacin 10-one [6] and 18/21-cis-annomontacin-10-one [7], two semi-synthetic mono-THF acetogenins were prepared from 1. These acetogenins showed cytotoxicity, comparable or superior to adriamycin, against three human solid tumor cell lines. Reduction of the 10-keto of 1 to the racemic OH-10 derivative enhanced the bioactivity, as did the conversion of 1 to 6 and 7. Venezenin [1], like other Annonaceous acetogenins, showed inhibition of oxygen uptake by rat liver mitochondria and demonstrated that the THF ring may not be essential to this mode of action. PMID:7623031

  9. Inhibitory effects of Curcuma aromatica oil on proliferation of hepatoma in mice

    Institute of Scientific and Technical Information of China (English)

    Wan Yin Wu; Qin Xu; Ling Chun Shi; Wei Bin Zhang

    2000-01-01

    AIM To reveal the inhibitory effects of Curcuma aromatica oil ( CAO ) on cell proliferation of hepatoma in mice. METHODS Two tumor inhibitory experiments of CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation of hepatoma in mice were evaluated by DNA image cytometry and immunohistochemical staining of proliferating cell nuclear antigen (PCNA).RESULTS The tumor inhibitory rates of CAO were 52% and 51% in two experiments,respectively. Compared with those of the salinetreated control groups, both differences were statistically significant (P < 0.01). In the group of mice treated with CAO, the cellular nuclear DNA OD value (249 ± 70), areas (623μnm2 ±228 μm2) and DNA (2.38 ± 0.67) index of hepatic carcinomas were significantly lower than those of the control group (430 ± 160, 1073μm2 ± 101 um2 and 4.48 ± 0.71 ). CAO also could increase diploidy cell rates (29.00% ± 9.34% vs 2.97% ± 5.69%, P<0.01 ) and decrease pentaploidy cell exceeding rate (30.04% ± 15.10% vs 70.89%±14.94%, P<0.01). In the group of mice treated with CAO, the labeling indexes of proliferating cell nuclear antigen (PCNA-LI) were 30% ± 4%, which were significantly lower than 40% ± 6% of the control group (P<0.01). CONCLUSION The inhibition of CAO on the growth of hepatoma in mice might be associated with its depression on cellular proliferative activity.

  10. Safety of Curcuma aromatica oil gelatin microspheres administered via hepatic artery

    Institute of Scientific and Technical Information of China (English)

    Shi-Gui Deng; Zhi-Feng Wu; Wei-Ying Li; Zhi-Gang Yang; Gang Chang; Fan-Zhe Meng; Li-Li Mo

    2004-01-01

    AIM: To evaluate the safety of Curcuma aromatica oilgelatin microspheres (CAO-GMS) infused via hepatic artery against primary liver cancer.METHODS: The safety of CAO-GMS was evaluated in view of its acute toxicity in rats, long-term toxicity in Beagle dogs and general pharmacology in rats and mongrel dogs. RESULTS: The 50% lethal dose (LD50) of CAO-GMS infusedvia the hepatic artery was 17.19 mg/kg, and the serum biochemical indices of dying rats after the administration changed markedly while those of survived rats did not. Subsequent pathological examination of the tissues from the dead rats indicated improper embolism. Similar edema and small necrotic foci in the hepatic Iobule were found in the hepatic tissue of rats receiving 10 and 5 mg/kg CAO GMS and GMS 60 d after the last administration, while not in the rats of the blank control group, indicating that microspheres infused via the hepatic artery may induce irreversible liver damage dose-dependently. General pharmacological study showed that the activities (postureand gait), respiration frequency, blood pressure or heart rate of the dogs were not affected by CAO-GMS, nor were salivation, tremor or pupil changes of the rats observed or their balancing ability compromised, suggesting CAO-GMSinfused via the hepatic artery did not significantly affect the nervous, respiratory and cardiovascular systems. CONCLUSION: CAO-GMS embolization administered via the hepatic artery is safe but undesired embolization induced by vascular variation should be given due attention in its clinical application. Individualized embolization dosage and super-selective catheterization technique are recommended to avoid undesired embolism and reduce complications.

  11. Effects of environmental conditions associated to the cardinal orientation on the reproductive phenology of the cerrado savanna tree Xylopia aromatica (Annonaceae).

    Science.gov (United States)

    Camargo, Maria Gabriela G; Souza, Regina M; Reys, Paula; Morellato, Leonor P C

    2011-09-01

    The Brazilian cerrado has undergone an intense process of fragmentation, which leads to an increase in the number of remnants exposed to edge effects and associated changes on environmental conditions that may affect the phenology of plants. This study aimed to verify whether the reproductive phenology of Xylopia aromatica (Lam.) Mart. (Annonaceae) differs under different light conditions in a cerrado sensu stricto (a woody savanna) of southeastern Brazil. We compared the reproductive phenology of X. aromatica trees distributed on east and south cardinal faces of the cerrado during monthly observations, from January 2005 to December 2008. The east face had a higher light incidence, higher temperatures and canopy openness in relation to south face. X. aromatica showed seasonal reproduction at both faces of the cerrado, but the percentage of individuals, the synchrony and duration of phenophases were higher at the east face. The study demonstrated the influence of the environmental conditions associated to the cardinal orientation of the cerrado faces on the phenological pattern of X. aromatica. Similar responses may be observed for other species, ultimately affecting patterns of floral visitation and fruit production, which reinforces the importance of considering the cardinal direction in studies of edge effects and fragmentation.

  12. Genetic Variability of the Narrow Endemic Tree Antirhea aromatica Castillo‐Campos & Lorence, (Rubiaceae, Guettardeae) in a Tropical Forest of Mexico

    OpenAIRE

    GONZÁLEZ‐ASTORGA, JORGE; CASTILLO‐CAMPOS, GONZALO

    2004-01-01

    • Background and Aims Genetic structure and variability were examined in the only three extant populations of the narrow‐endemic tree Antirhea aromatica (Rubiaceae, Guettardeae), an endangered species of the tropical forest of eastern Mexico. Patterns of genetic diversity within and among populations for adult plants and seedlings were obtained.

  13. NCBI nr-aa BLAST: CBRC-XTRO-01-0459 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0459 ref|YP_286542.1| Acriflavin resistance protein [Dechloromonas aro...matica RCB] gb|AAZ48072.1| Acriflavin resistance protein [Dechloromonas aromatica RCB] YP_286542.1 0.0 62% ...

  14. NCBI nr-aa BLAST: CBRC-TTRU-01-0586 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0586 ref|YP_284494.1| hypothetical protein Daro_1272 [Dechloromonas ar...omatica RCB] gb|AAZ46024.1| hypothetical protein Daro_1272 [Dechloromonas aromatica RCB] YP_284494.1 1.8 23% ...

  15. NCBI nr-aa BLAST: CBRC-XTRO-01-0032 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0032 ref|YP_286391.1| Cytochrome c oxidase, subunit I [Dechloromonas a...romatica RCB] gb|AAZ47921.1| Cytochrome c oxidase, subunit I [Dechloromonas aromatica RCB] YP_286391.1 1e-118 67% ...

  16. NCBI nr-aa BLAST: CBRC-XTRO-01-0450 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0450 ref|YP_283798.1| Sulphate anion transporter [Dechloromonas aromat...ica RCB] gb|AAZ45328.1| Sulphate anion transporter [Dechloromonas aromatica RCB] YP_283798.1 1e-87 46% ...

  17. NCBI nr-aa BLAST: CBRC-XTRO-01-0508 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0508 ref|YP_285565.1| inner-membrane translocator [Dechloromonas aroma...tica RCB] gb|AAZ47095.1| inner-membrane translocator [Dechloromonas aromatica RCB] YP_285565.1 1e-110 73% ...

  18. NCBI nr-aa BLAST: CBRC-TGUT-17-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-17-0005 ref|YP_285627.1| Membrane bound O-acyl transferase, MBOAT [Dechloromo...nas aromatica RCB] gb|AAZ47157.1| Membrane bound O-acyl transferase, MBOAT [Dechloromonas aromatica RCB] YP_285627.1 0.72 28% ...

  19. NCBI nr-aa BLAST: CBRC-XTRO-01-0431 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0431 ref|YP_283966.1| Flagellar biosynthesis protein FlhA [Dechloromon...as aromatica RCB] gb|AAZ45496.1| Flagellar biosynthesis protein FlhA [Dechloromonas aromatica RCB] YP_283966.1 0.0 58% ...

  20. NCBI nr-aa BLAST: CBRC-XTRO-01-0305 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-XTRO-01-0305 ref|YP_285817.1| Heavy metal efflux pump CzcA [Dechloromonas arom...atica RCB] gb|AAZ47347.1| Heavy metal efflux pump CzcA [Dechloromonas aromatica RCB] YP_285817.1 0.0 74% ...

  1. Effects of environmental conditions associated to the cardinal orientation on the reproductive phenology of the cerrado savanna tree Xylopia aromatica (Annonaceae

    Directory of Open Access Journals (Sweden)

    Maria Gabriela G. Camargo

    2011-09-01

    Full Text Available The Brazilian cerrado has undergone an intense process of fragmentation, which leads to an increase in the number of remnants exposed to edge effects and associated changes on environmental conditions that may affect the phenology of plants. This study aimed to verify whether the reproductive phenology of Xylopia aromatica (Lam. Mart. (Annonaceae differs under different light conditions in a cerrado sensu stricto (a woody savanna of southeastern Brazil. We compared the reproductive phenology of X. aromatica trees distributed on east and south cardinal faces of the cerrado during monthly observations, from January 2005 to December 2008. The east face had a higher light incidence, higher temperatures and canopy openness in relation to south face. X. aromatica showed seasonal reproduction at both faces of the cerrado, but the percentage of individuals, the synchrony and duration of phenophases were higher at the east face. The study demonstrated the influence of the environmental conditions associated to the cardinal orientation of the cerrado faces on the phenological pattern of X. aromatica. Similar responses may be observed for other species, ultimately affecting patterns of floral visitation and fruit production, which reinforces the importance of considering the cardinal direction in studies of edge effects and fragmentation.O cerrado brasileiro vem sofrendo um processo intenso de fragmentação, que levou ao aumento de remanescentes sujeitos aos efeitos de borda e a alterações nas condições ambientais que podem afetar a fenologia das plantas. O objetivo deste trabalho foi verificar se a fenologia reprodutiva de Xylopia aromatica (Lam. Mart. (Annonaceae difere sob diferentes condições de luz em um cerrado sensu stricto no sudeste do Brasil. Comparamos a fenologia reprodutiva de árvores de X. aromatica distribuídas nas faces leste e sul do cerrado, observadas mensalmente de janeiro de 2005 a dezembro de 2008. A face leste apresentou

  2. House for Niels Strøyberg

    DEFF Research Database (Denmark)

    Welling, Helen

    2007-01-01

    The house for Niels Strøyberg in Hasseris near Aalborg, Denmark, is a notable example of early modernism in an unconventional way. Although Poul Henningsen's approach to the dwelling was thoroughly functional, he added a number of visual effects to provide a sense of dynamism to the spatial...

  3. Y chromosomal STR analysis using Pyrosequencing technology.

    Science.gov (United States)

    Edlund, Hanna; Allen, Marie

    2009-03-01

    Analysis of Y chromosome STR markers has proven to be useful in forensic cases where the samples contain a mixture of DNA from several individuals. STR markers are commonly genotyped based on length separation of PCR products. In this study we evaluated if Pyrosequencing can be used as an alternative method for determining Y-STR variants. In total 70 unrelated Swedish males were typed for the Y chromosomal markers (DYS19, DYS389 I-II, DYS390, DYS391, DYS392, DYS393 and DYS438) using Pyrosequencing. Using the 8 markers, 57 unique haplotypes were observed with a discrimination capacity of 0.81. At four loci, the Pyrosequencing analysis revealed sequence variants. The sequence variants were found in the DYS389 II, DYS390, DYS391, and DYS393 loci in frequencies between 1.43% and 14.3%. Pyrosequencing has here been shown to be a useful tool for typing Y chromosomal STRs and the method can provide a complement to conventional forensic Y STR analyses. Moreover, the Pyrosequencing method can be used to rapidly evaluate novel markers. PMID:19215881

  4. 温莪术内生真菌的分离及其分类鉴定%Isolation and Identification of Endophytic Fungi from Curcuma aromatica Salisb

    Institute of Scientific and Technical Information of China (English)

    王艳红; 汪洪; 徐磊; 杨信东

    2009-01-01

    Objective To isolate and identify the endophytic fungi of different positions in Curcuma aromatica Salisb. Method-S Endophytic fungi were isolated from healthy roots, stems and leaves of Curcuma aromatical Salisb and identified with conventional method. Results Ninty - nine strains of endophytic fungi belong to 21 genus were isolated and morhpologically identified from Curcuma aromatica Salisb. Forty -two strains of 11 genera were isolated in roots, 12strains of 4 genera in stems,and 45 strains of 12 genera in leaves. Conclusion The quantity,population and distribution of the endophytic fungi are various in different position of Curcuma aromatica Salisb.%目的 对温莪术不同部位内生真菌进分离和分类鉴定.方法 采用内生菌常规分离法对健康温莪术根、茎和叶的内生真菌进行了分离和鉴定.结果 从温莪术中分离得到了99株内生真菌,经鉴定为21属,其中根部42株涉及11属,茎部12株涉及4属,叶部45株涉及12属.结论 温莪术的不同部位内生真菌的数量、分布和种群存在差异.

  5. Aqueous extract of Curcuma aromatica induces apoptosis and G2/M arrest in human colon carcinoma LS-174-T cells independent of p53.

    Science.gov (United States)

    Hu, Bing; Shen, Ke-Ping; An, Hong-Mei; Wu, Yang; Du, Qin

    2011-02-01

    Curcuma aromatica is a common Chinese herb for treating diseases with blood stasis and has been regarded as an anticancer herb in modern clinical practice. However, the anticancer effects and related molecular mechanisms of Curcuma aromatica remain unclear. In the present study, human colon carcinoma LS-174-T cell line with wild-type p53 was used as a model cell to evaluate the anticancer effects of aqueous extract of Curcuma aromatica (AECA). AECA inhibits LS-174-T cell proliferation in a dose- and time-dependent manner and colony formation in a dose-dependent manner. AECA treatment induces apoptosis accompanied by caspase-8, -9, and -3 activation in LS-174-T cells. Moreover, blocking the activities of these caspases with a specific inhibitor significantly protected LS-174-T cells from AECA-induced apoptosis. AECA treatment also induces G2/M phase arrest in LS-174-T cells. Expression of p53 was unchanged after AECA treatment; specific silence of p53 did not influence AECA-induced apoptosis and G2/M phase arrest. Further, the expression of cyclin B1 and CDK1 was reduced by AECA. This study suggests that AECA might be effective as an antiproliferative herb for colon carcinoma, the antitumor activity of AECA may involve both extrinsic and intrinsic apoptosis, and AECA induces G2/M phase arrest via downregulation of cyclin B1 and CDK1 and without the participation of p53.

  6. Interpreting Y chromosome STR haplotype mixture.

    Science.gov (United States)

    Ge, Jianye; Budowle, Bruce; Chakraborty, Ranajit

    2010-05-01

    Mixture interpretation is a challenging problem in forensic DNA analyses. The interpretation of Y short tandem repeat (STR) haplotype mixtures, due to a lack of recombination, differs somewhat from that of the autosomal DNA markers and is more complex. We describe approaches for calculating the probability of exclusion (PE) and likelihood ratio (LR) methods to interpret Y-STR mixture evidence with population substructure incorporated. For a mixture sample, first, all possible contributor haplotypes in a reference database are listed as a candidate list. The PE is the complement of the summation of the frequencies of haplotypes in the candidate list. The LR method compares the probabilities of the evidence given alternative hypotheses. The hypotheses are possible explanations for the mixture. Population substructure may be further incorporated in likelihood calculation. The maximum number of contributors is based on the candidate list and the computing complexity is polynomial. Additionally, mixtures were simulated by combining two or three 16 Y-STR marker haplotypes derived from the US forensic Y-STR database. The average PE was related to the size of database. With a database comprised of 500 haplotypes an average PE value of at least 0.995 can be obtained for two-person mixtures. The PE decreases with an increasing number of contributors to the mixture. Using the total sample population, the average number of candidate haplotypes of two-person mixtures is 3.73 and 95% mixtures have less than or equal to 10 candidate haplotypes. More than 98.7% of two-person mixtures can only be explained by the haplotype combinations that mixtures are composed. These values are generally higher for three-person mixtures. A small proportion of three-person mixture can also be explained by only two haplotypes.

  7. Associations between STR autosomal markers and longevity.

    Science.gov (United States)

    Bediaga, N G; Aznar, J M; Elcoroaristizabal, X; Albóniga, O; Gómez-Busto, F; Artaza Artabe, I; Rocandio, Ana; de Pancorbo, M M

    2015-10-01

    Life span is a complex and multifactorial trait, which is shaped by genetic, epigenetic, environmental, and stochastic factors. The possibility that highly hypervariable short tandem repeats (STRs) associated with longevity has been largely explored by comparing the genotypic pools of long lived and younger individuals, but results so far have been contradictory. In view of these contradictory findings, the present study aims to investigate whether HUMTHO1 and HUMCSF1PO STRs, previously associated with longevity, exert a role as a modulator of life expectancy, as well as to assess the extent to which other autosomal STR markers are associated with human longevity in population from northern Spain. To that end, 21 autosomal microsatellite markers have been studied in 304 nonagenarian individuals (more than 90 years old) and 516 younger controls of European descent. Our results do not confirm the association found in previous studies between longevity and THO1 and CSF1PO loci. However, significant association between longevity and autosomal STR markers D12S391, D22S1045, and DS441 was observed. Even more, when we compared allelic frequency distribution of the 21 STR markers between cases and controls, we found that 6 out of the 21 STRs studied showed different allelic frequencies, thus suggesting that the genomic portrait of the human longevity is far complex and probably shaped by a high number of genomic loci. PMID:26335621

  8. Validation of STR typing by capillary electrophoresis.

    Science.gov (United States)

    Moretti, T R; Baumstark, A L; Defenbaugh, D A; Keys, K M; Brown, A L; Budowle, B

    2001-05-01

    With the use of capillary electrophoresis (CE), high-resolution electrophoretic separation of short tandem repeat (STR) loci can be achieved in a semiautomated fashion. Laser-induced detection of fluorescently labeled PCR products and multicolor analysis enable the rapid generation of multilocus DNA profiles. In this study, conditions for typing PCR-amplified STR loci by capillary electrophoresis were investigated using the ABI Prism 310 Genetic Analyzer (Applied Biosystems). An internal size standard was used with each run to effectively normalize mobility differences among injections. Alleles were designated by comparison to allelic ladders that were run with each sample set. Multiple runs of allelic ladders and of amplified samples demonstrate that allele sizes were reproducible, with standard deviations typically less than 0.12 bases for fragments up to 317 bases in length (largest allele analyzed) separated in a 47 cm capillary. Therefore, 99.7% of all alleles that are the same length should fall within the measurement error window of +/- 0.36 bases. Microvariants of the tetranucleotide repeats were also accurately typed by the analytical software. Alleles differing in size by one base could be resolved in two-donor DNA mixtures in which the minor component comprised > or = 5% of the total DNA. Furthermore, the quantitative data format (i.e., peak amplitude) can in some instances assist in determining individual STR profiles in mixed samples. DNA samples from previously typed cases (typed for RFLP, AmpliType PM+DQA1, and/or D1S80) were amplified using AmpFlSTR Profiler Plus and COfiler and were evaluated using the ABI Prism 310. Most samples yielded typable results. Compared with previously determined results for other loci, there were no discrepancies as to the inclusion or exclusion of suspects or victims. CE thus provides efficient separation, resolution, sensitivity and precision, and the analytical software provides reliable genotyping of STR loci. The

  9. Modulation of antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis.

    Science.gov (United States)

    Panich, Uraiwan; Kongtaphan, Kamolratana; Onkoksoong, Tassanee; Jaemsak, Kannika; Phadungrakwittaya, Rattana; Thaworn, Athiwat; Akarasereenont, Pravit; Wongkajornsilp, Adisak

    2010-04-01

    Ultraviolet A (UVA) irradiation is suggested to contribute to melanogenesis through promoting cellular oxidative stress and impairing antioxidant defenses. An overproduction of melanin can be associated with melanoma skin cancer and hyperpigmentation. Therefore, developing effective antimelanogenic agents is of importance. Alpinia galanga (AG) and Curcuma aromatica (CA) are traditional medicinal plants widely used for skin problems. Hence, this study investigated the antimelanogenic effects of AG and CA extracts (3.8-30 microg/ml) by assessing tyrosinase activity, tyrosinase mRNA levels, and melanin content in human melanoma cells (G361) exposed to UVA. The roles in protecting against melanogenesis were examined by evaluating their inhibitory effects on UVA-induced cellular oxidative stress and modulation of antioxidant defenses including antioxidant enzymes, catalase (CAT) and glutathione peroxidase (GPx), and intracellular glutathione (GSH). In addition, possible active compounds accountable for biological activities of the extracts were identified by thin layer chromatography (TLC)-densitometric analysis. Our study demonstrated that UVA (8 J/cm(2)) induced both tyrosinase activity and mRNA levels and UVA (16 J/cm(2))-mediated melanin production were suppressed by the AG or CA extracts at noncytotoxic concentrations. Both extracts were able to protect against UVA-induced cellular oxidant formation and depletion of CAT and GPx activities and GSH content in a dose-dependent manner. Moreover, TLC-densitometric analysis detected the presence of eugenol and curcuminoids in AG and CA, respectively. This is the first report representing promising findings on AG and CA extract-derived antityrosinase properties correlated with their antioxidant potential. Inhibiting cellular oxidative stress and improving antioxidant defenses might be the mechanisms by which the extracts yield the protective effects on UVA-dependent melanogenesis.

  10. Can brothers share the same STR profile?

    Science.gov (United States)

    Zaken, Naomi; Motro, Uzi; Berdugo, Reouven; Sapir, Liron Elkayam; Zamir, Ashira

    2013-09-01

    This report demonstrates the limits of DNA identification when siblings are involved. The Israeli DNA database routinely amplifies suspects samples using the PowerPlex(®) ESI16 system (Promega). While uploading a series of suspects into the database software, we found an unusual high number of shared alleles between two suspects 31 out of 32 alleles. Verification of their demographic data identified them as brothers. After confirmation of their paternity affiliation using the AmpFlSTR(®)YFiler™ (Applied Biosystems), we used two other multiplexes kits to improve the differentiation rate. The PowerPlex(®) ESX17 System (Promega) added one locus, SE33, who exhibits four different alleles. The second kit, the AmpFlSTR(®)MiniFiler™ (Applied Biosystems) added three more loci. Only one allele difference was found. In order to increase the discrimination power between related and unrelated individuals, we recommend that the DNA laboratories consider using a larger multiplex typing kit in cases like the one informed here.

  11. Y-STR frequency surveying method

    DEFF Research Database (Denmark)

    Willuweit, Sascha; Caliebe, Amke; Andersen, Mikkel Meyer;

    2011-01-01

    Reasonable formalized methods to estimate the frequencies of DNA profiles generated from lineage markers have been proposed in the past years and were discussed in the forensic community. Recently, collections of population data on the frequencies of variations in Y chromosomal STR profiles have...... reached a new quality with the establishment of the comprehensive neatly quality-controlled reference database YHRD. Grounded on such unrivalled empirical material from hundreds of populations studies the core assumption of the Haplotype Frequency Surveying Method originally described 10 years ago can...... be tested and improved. Here we provide new approaches to calculate the parameters used in the frequency surveying method: a maximum likelihood estimation of the regression parameters (r1, r2, s1 and s2) and a revised Frequency Surveying framework with variable binning and a database preprocessing to take...

  12. Y-chromosomal STR haplotypes in Central Thai population.

    Science.gov (United States)

    Siriboonpiputtana, T; Jomsawat, U; Rinthachai, T; Thanakitgosate, J; Shotivaranon, J; Limsuwanachot, N; Polyorat, P; Rerkamnuaychoke, B

    2010-04-01

    12 Y-STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439 and DYS437) were typed with PowerPlex Y System (Promega, USA) in a total sample of 501 unrelated males from the central part of Thailand. Allele frequencies and gene diversity for each Y-STR locus were determined. Haplotype diversity from the combined 12 Y-STR loci was 0.9996. The present results can be used as Thai ethnic genetic information resources in routine forensic analysis. PMID:20215020

  13. Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition.

    Science.gov (United States)

    Duldhardt, Ilka; Gaebel, Julia; Chrzanowski, Lukasz; Nijenhuis, Ivonne; Härtig, Claus; Schauer, Frieder; Heipieper, Hermann J

    2010-03-01

    The effect of different solvents and pollutants on the cellular fatty acid composition of three bacterial strains: Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans, representatives of diverse predominant anaerobic metabolisms was investigated. As the prevailing adaptive mechanism in cells of T. aromatica and G. sulfurreducens whose cellular fatty acids patterns were dominated by palmitic acid (C16:0) and palmitoleic acid (C16:1cis), the cells reacted by an increase in the degree of saturation of their membrane fatty acids when grown in the presence of sublethal concentrations of the chemicals. Next to palmitic acid C16:0, the fatty acid pattern of D. multivorans was dominated by anteiso-branched fatty acids which are characteristic for several sulfate-reducing bacteria. The cells responded to the solvents with an increase in the ratio of straight-chain saturated (C14:0, C16:0, C18:0) to anteiso-branched fatty acids (C15:0anteiso, C17:0anteiso, C17:1anteisoΔ9cis). The results show that anaerobic bacteria react with similar mechanisms like aerobic bacteria in order to adapt their membrane to toxic organic solvents. The observed adaptive modifications on the level of membrane fatty acid composition can only be carried out with de novo synthesis of the fatty acids which is strictly related to cell growth. As the growth rates of anaerobic bacteria are generally much lower than in the so far investigated aerobic bacteria, this adaptive response needs more time in anaerobic bacteria. This might be one explanation for the previously observed higher sensitivity of anaerobic bacteria when compared with aerobic ones. PMID:21255320

  14. Evaluation of population variation at 17 autosomal STR and 16 Y-STR haplotype loci in Croatians.

    Science.gov (United States)

    Haliti, Naim; Carapina, Mirela; Masić, Marijana; Strinović, Davor; Klarić, Irena Martinović; Kubat, Milovan

    2009-09-01

    Seventeen autosomal STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA, Penta E and Penta D) and 16 Y-STR haplotype loci (DYS19, DYS385, DYS389I, DYS398II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4.1) were analyzed in the sample of 200 unrelated Croatians. The agreement with HWE was confirmed for all autosomal STR loci. The combined power of discrimination (PD) and the combined power of exclusion (PE) for the 17 autosomal STR loci were 0.999999999999999999682299331476 and 0.99999995, respectively. Penta E proved to be the most informative autosomal STR locus. Among 200 Croatian males, 197 Y-STR haplotypes were identified and haplotype diversity was estimated at 0.9998+/-0.0005. PMID:19647700

  15. Using probabilistic theory to develop interpretation guidelines for Y-STR profiles.

    Science.gov (United States)

    Taylor, Duncan; Bright, Jo-Anne; Buckleton, John

    2016-03-01

    Y-STR profiling makes up a small but important proportion of forensic DNA casework. Often Y-STR profiles are used when autosomal profiling has failed to yield an informative result. Consequently Y-STR profiles are often from the most challenging samples. In addition to these points, Y-STR loci are linked, meaning that evaluation of haplotype probabilities are either based on overly simplified counting methods or computationally costly genetic models, neither of which extend well to the evaluation of mixed Y-STR data. For all of these reasons Y-STR data analysis has not seen the same advances as autosomal STR data. We present here a probabilistic model for the interpretation of Y-STR data. Due to the fact that probabilistic systems for Y-STR data are still some way from reaching active casework, we also describe how data can be analysed in a continuous way to generate interpretational thresholds and guidelines.

  16. Using probabilistic theory to develop interpretation guidelines for Y-STR profiles.

    Science.gov (United States)

    Taylor, Duncan; Bright, Jo-Anne; Buckleton, John

    2016-03-01

    Y-STR profiling makes up a small but important proportion of forensic DNA casework. Often Y-STR profiles are used when autosomal profiling has failed to yield an informative result. Consequently Y-STR profiles are often from the most challenging samples. In addition to these points, Y-STR loci are linked, meaning that evaluation of haplotype probabilities are either based on overly simplified counting methods or computationally costly genetic models, neither of which extend well to the evaluation of mixed Y-STR data. For all of these reasons Y-STR data analysis has not seen the same advances as autosomal STR data. We present here a probabilistic model for the interpretation of Y-STR data. Due to the fact that probabilistic systems for Y-STR data are still some way from reaching active casework, we also describe how data can be analysed in a continuous way to generate interpretational thresholds and guidelines. PMID:26706152

  17. Reproducing the observed abundances in RCB and HdC stars with post-double degenerate merger models - constraints on merger and post-merger simulations and physics processes

    CERN Document Server

    Menon, Athira; Denissenkov, Pavel A; Clayton, Geoffrey C; Staff, Jan; Pignatari, Marco; Paxton, Bill

    2012-01-01

    The R Coronae Borealis (RCB) stars along with the H-deficient Carbon stars (HdCs) and Extreme Helium stars (EHes), are believed to be the results of He-CO WD mergers. Their H-poor, C-rich atmospheres are characterized by very unusual isotopic ratios such as extremely low O16/O18 ~ 1 - 10, C12/C13 >= 100, and enhancements up to 2.6 dex in F and in s-process elements from Zn to La, compared to solar. As shown before such O-isotopic ratios cannot result from the dynamic double-degenerate merger phase. We therefore investigate now the role of the long-term post-merger evolution and nucleosynthesis. We construct post-merger 1D spherical models based on realistic merger progenitor models and the outcome of our previous hydrodynamic simulations and follow their evolution into the RCB domain of the HR diagram. Along with surface convection zones, we adopt a model for extra mixing that is meant to represent processes driven by rotation originating in the dynamical merger. We perform complete multi-zone post-processing...

  18. Genetic Diversity in Gorkhas: an Autosomal STR Study.

    Science.gov (United States)

    Preet, Kiran; Malhotra, Seema; Shrivastava, Pankaj; Jain, Toshi; Rawat, Shweta; Varte, L Robert; Singh, Sayar; Singh, Inderjeet; Sarkar, Soma

    2016-01-01

    Genotyping of highly polymorphic autosomal short tandem repeat (STR) markers is a potent tool for elucidating genetic diversity. In the present study, fifteen autosomal STR markers were analyzed in unrelated healthy male Gorkha individuals (n = 98) serving in the Indian Army by using AmpFlSTR Identifiler Plus PCR Amplification Kit. In total, 138 alleles were observed with corresponding allele frequencies ranging from 0.005 to 0.469. The studied loci were in Hardy-Weinberg Equilibrium (HWE). Heterozygosity ranged from 0.602 to 0.867. The most polymorphic locus was Fibrinogen Alpha (FGA) chain which was also the most discriminating locus as expected. Neighbor Joining (NJ) tree and principal component analysis (PCA) plot clustered the Gorkhas with those of Nepal and other Tibeto-Burman population while lowlander Indian population formed separate cluster substantiating the closeness of the Gorkhas with the Tibeto-Burman linguistic phyla. Furthermore, the dataset of STR markers obtained in the study presents a valuable information source of STR DNA profiles from personnel for usage in disaster victim identification in military exigencies and adds to the Indian database of military soldiers and military hospital repository. PMID:27580933

  19. GENETIC POLYMORPHISM OF STR LOCI IN CHINESE DRUNGS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Objective:To study the STR polymorphism in Chinese Drungs.Methods:The genetic distributions of 15 STR loci and Amelogenin locus were generated through coamplification,genescan and genotype from 65 Drungs Results:There were 144 STR and 2 Amelogenin alleles in Drung nationality,with their frequencies ranging from 0.0077 to 0.7846,H0.3723-0.8639,DP0.5567-0.9548,EPP0.2738-0.8358,and PIC 0.3461-0.8456,the accumulative DP 0.9999998and EPP 0.9999894,Conclusion:The study founded a basis for the genetic structure of Chinese ethnic groups ,which is useful for the application to anthropology and forensic science.

  20. GENETIC POLYMORPHISM OF STR LOCI IN CHINESE DRUNGS

    Institute of Scientific and Technical Information of China (English)

    赖江华; 郑海波; 朱波峰; 李生斌

    2002-01-01

    Objective To study the STR polymorphism in Chi nese Drungs. Methods The genetic distributions of 15 STR loci a nd Amelogenin locus were generated through coamplification, genescan and genotyp e from 65 Drungs. Results There were 144 STR and 2 Amelogenin alleles in Drung n ationality, with their frequencies ranging from 0.0077 to 0.7846, H 0.3723~0.8 639, DP 0.5567~0.9548, EPP 0.2738~0.8358, and PIC 0.3461~0.8456, the accumul ative DP 0.99999998 and EPP 0.99999894. Conclusion The study f ounded a basis for the genetic structure of Chinese ethnic group s,which is useful for the application to anthropology and forensic science.

  1. A comparison of SNP and STR loci for delineating population structure and performing individual genetic assignment

    DEFF Research Database (Denmark)

    Glover, Kevin A.; Hansen, Michael Møller; Lien, Sigbjørn;

    2010-01-01

    between SNP and STR data sets and variants thereof. The best 15 SNPs (30 alleles) gave a similar level of self-assignment to the best 4 STR loci (83 alleles), however, addition of further STR loci did not lead to a notable increase assignment whereas addition of up to 100 SNP loci increased assignment...... relationships at the individual and population levels....

  2. STR data for the 13 CODIS loci in Singapore Malays.

    Science.gov (United States)

    Ang, H C; Sornarajah, R; Lim, S E S; Syn, C K C; Tan-Siew, W F; Chow, S T; Budowle, Bruce

    2005-03-10

    Allele frequencies for the 13 CODIS (Combined DNA Index System, USA) STR loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 197 unrelated Malays in Singapore.

  3. Stochastic filtering of quantitative data from STR DNA analysis

    DEFF Research Database (Denmark)

    Tvedebrink, Torben; Eriksen, Poul Svante; Mogensen, Helle Smidt;

    The quantitative data observed from analysing STR DNA is a mixture of contributions from various sources. Apart from the true allelic peaks, the observed signal consists of at least three components resulting from the measurement technique and the PCR amplification: Background noise (random noise...

  4. A 27-locus STR assay to meet all United States and European law enforcement agency standards.

    Science.gov (United States)

    Schumm, James W; Gutierrez-Mateo, Cristina; Tan, Eugene; Selden, Richard

    2013-11-01

    Different national and international agencies have selected specific STR sets for forensic database use. To enhance database comparison across national and international borders, a 27-locus multiplex system was developed comprising all 15 STR loci of the European standard set, the current 13 STR loci of the CODIS core, the proposed 22 STR loci of the expanded CODIS core, 4 additional commonly used STR loci, and the amelogenin locus. Development required iterative primer design to resolve primer-related artifacts, amplicon sizing, and locus-to-locus balance issues. The 19.5-min assay incorporated newly developed six-dye chemistry analyzed using a novel microfluidic electrophoresis instrument capable of simultaneous detection and discrimination of 8 or more fluorescent dyes. The 27-locus multiplex offers the potential for a new international STR standard permitting laboratories in any jurisdiction to use a single reaction to determine profiles for loci they typically generate plus an expanded common STR profiling set of global interest.

  5. Informativeness of the CODIS STR loci for admixture analysis.

    Science.gov (United States)

    Barnholtz-Sloan, Jill S; Pfaff, Carrie L; Chakraborty, Ranajit; Long, Jeffrey C

    2005-11-01

    Population admixture (or ancestry) is used as an approach to gene discovery in complex diseases, particularly when the disease prevalence varies widely across geographic populations. Admixture analysis could be useful for forensics because an indication of a perpetrator's ancestry would narrow the pool of suspects for a particular crime. The purpose of this study was to use Fisher's information to identify informative sets of markers for admixture analysis. Using published founding population allele frequencies we test three marker sets for efficacy for estimating admixture: the FBI CODIS Core STR loci, the HGDP-CEPH Human Genome Diversity Cell Line Panel and the set of 39 ancestry informative SNPS from the Shriver lab at Pennsylvania State University. We conclude that the FBI CODIS Core STR set is valid for admixture analysis, but not the most precise. We recommend using a combination of the most informative markers from the HGDP-CEPH and Shriver loci sets.

  6. CODIS STR loci data from 41 sample populations.

    Science.gov (United States)

    Budowle, B; Shea, B; Niezgoda, S; Chakraborty, R

    2001-05-01

    Allele distributions for 12 or 13 CODIS core tetrameric short tandem repeat (STR) loci CSFIPO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA were determined in 41 population data sets. The major population groups comprise African Americans, U.S. Caucasians, Hispanics, Far East Asians, and Native Americans. There was little evidence for departures from Hardy-Weinberg expectations (HWE) in any of the populations. The FST estimates over all thirteen STR loci are 0.0006 for African Americans, -0.0005 for Caucasians, 0.0021 for Hispanics, 0.0039 for Asians, and 0.0282 for Native Americans.

  7. Numerische Simulation der aktiven Strömungsbeeinflussung in Turbomaschinen

    OpenAIRE

    Wunderer, Roland

    2010-01-01

    Im Rahmen dieser Arbeit wurde nachgewiesen, daß das Betriebsverhalten einer Francis Turbine im extremen Teillastbetrieb durch die Anregung der Strömung mit Hilfe schwingender Vorleitschaufeln verbessert werden kann. Die Vorleitschaufeln schwingen dabei mit bestimmten Frequenzen und Amplituden um ihre Aufhängungsachsen. Es wurde gezeigt, daß dadurch einerseits der hydraulische Wirkungsgrad gesteigert werden kann und andererseits sowohl die Amplituden der Druckschwankungen reduziert als auch de...

  8. Optimalizace webových stránek

    OpenAIRE

    Bača, Roman

    2008-01-01

    Tématem této bakalářské práce je optimalizace webových stránek pro vyhledávače (SEO), její realizace a popis činitelů, které na ni mají zásadní vliv. V prvním oddílu práce je pro lepší pochopení SEO přiblížena historie vyhledávačů a SEO, zásady pro tvorbu webových stránek a vysvětlen princip práce a struktura internetových vyhledávačů. Porovnává se zde také SEO s placenou formou reklamy PPC zaměřenou na zlepšení pozice stránky ve výsledcích vyhledávání. Po spíše teoreticky zaměřené části se p...

  9. Capillary electrophoresis of miniSTR markers to genotype highly degraded DNA samples.

    Science.gov (United States)

    Coble, Michael D

    2012-01-01

    The amplification of short tandem repeat (STR) markers throughout the human nuclear DNA genome are used to associate crime scene evidence to the perpetrator's profile in criminal investigations. For highly challenged or compromised materials such as stains exposed to the elements, skeletal remains from missing persons cases, or fragmented and degraded samples from mass disasters, obtaining a full STR profile may be difficult if not impossible. With the introduction of short amplicon STR or "miniSTR" typing, it is possible to obtain STR genetic information from highly challenged samples without the need to sequence the hypervariable regions of the mitochondrial DNA (mtDNA) genome. Non-Combined DNA Index System (CODIS) STR markers have been developed to obtain information beyond the core CODIS loci. This chapter will focus on the steps necessary to prepare and use one of the non-CODIS (NC) multiplexes, NC01 (Coble and Butler 2005), for analysis on capillary electrophoresis instrumentation.

  10. High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

    DEFF Research Database (Denmark)

    Fordyce, Sarah L; Avila-Arcos, Maria C; Rockenbauer, Eszter;

    2011-01-01

    The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR...... repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method...

  11. Croatian national reference Y-STR haplotype database.

    Science.gov (United States)

    Mršić, Gordan; Gršković, Branka; Vrdoljak, Andro; Popović, Maja; Valpotić, Ivica; Anđelinović, Šimun; Stenzl, Vlastimil; Ehler, Edvard; Urban, Ludvik; Lacković, Gordana; Underhill, Peter; Primorac, Dragan

    2012-07-01

    A reference Y-chromosome short tandem repeat (STR) haplotype database is needed for Y-STR match interpretation as well as for national and regional characterization of populations. The aim of this study was to create a comprehensive Y-STR haplotype database of the Croatian contemporary population and to analyze substructure between the five Croatian regions. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre "Ivan Vučetić". A total of 1,100 unrelated men from eastern, western, northern, southern and central Croatia were selected for the purpose of this study. Y-STRs were typed using the AmpFISTR Yfiler PCR amplification kit. Analysis of molecular variance calculated with the Y chromosome haplotype reference database online analysis tool included 16 population samples with 20,247 haplotypes. A total of 947 haplotypes were recorded, 848 of which were unique (89.5%). Haplotype diversity was 0.998, with the most frequent haplotype found in 9 of 1,100 men (0.82%). Locus diversity varied from 0.266 for DYS392 to 0.868 for DYS385. Discrimination capacity was 86.1%. Our results suggested high level of similarity among regional subpopulations within Croatia, except for mildly different southern Croatia. Relative resemblance was found with Bosnia and Herzegovina and Serbia. Whit Atheys' Haplogroup Predictor was used to estimate the frequencies of Y-chromosome haplogroups. I2a, R1a, E1b1b and R1b haplogroups were most frequent in all Croatian regions. These results are important in forensics and contribute to the population genetics and genetic background of the contemporary Croatian population. PMID:22391654

  12. Development of STR profiles from firearms and fired cartridge cases.

    Science.gov (United States)

    Horsman-Hall, Katie M; Orihuela, Yvette; Karczynski, Stephanie L; Davis, Ann L; Ban, Jeffrey D; Greenspoon, Susan A

    2009-09-01

    Fired cartridge cases are a common type of evidence found at crime scenes. However, due to the high chamber temperatures and touch nature of this evidence, DNA testing is not commonly sought because it is believed DNA is only present in low levels, whether it is due to initial low levels of DNA and/or DNA degradation from the heat or inhibition of the PCR reaction. Moreover, very few laboratories report STR typing success with fired cases. This study focused on obtaining STR profiles from fired cartridge cases using the AmpFlSTR MiniFiler kit, which is designed to amplify DNA from low level, inhibited, and degraded samples. Comparisons to other STR amplification kits were also conducted. In attempt to simulate casework, random individuals loaded cartridges into a firearm. DNA was recovered from the fired cartridge cases using the double swab technique and extracted using an automated large volume DNA IQ method. Initially, testing focused on known shedders handling cartridges for 30s prior to firing. A significantly greater number of alleles was obtained following amplification with the MiniFiler kit versus the PowerPlex 16 BIO kit. No alleles were observed using the Identifiler kit. In an attempt to better simulate casework, a random selection of laboratory personnel handled shotshells for as long as needed to load and fire the weapon. In this mock sample study, the MiniFiler kit successfully amplified an average of 22% of expected alleles from DNA recovered from shotshell cases versus the PowerPlex 16 BIO kit where an average of 7% of alleles were observed. However, the total number of alleles obtained from the two kits was not significantly different. The quality of the DNA obtained from fired cases was studied with evidence of inhibition in at least 11% of shotshell case samples. After swabbing the head and the hull of three shotshell cases separately, a significantly greater number of alleles was obtained from the hull as opposed to the head of the fired

  13. Cesta tvorbou webových stránek

    OpenAIRE

    Krčál Martin

    2012-01-01

    Článek v první části popisuje proces tvorby webových stránek od stanovení cílů a definování cílové skupiny přes návrh až po samotnou realizaci. Autor zdůrazňuje důležitost dostatečně velkého a nadšeného vývojového týmu a připomíná nezbytnost průběžného uživatelského testování. Zmiňovány jsou i některé nástroje pro testování stránek z pohledu SEO, použitelnosti a přístupnosti, stejně jako vzrůstající význam mobilních technologií. Druhá část článku je věnována uživatelskému testování a analzýze...

  14. Sousse, Tunisia: tumultuous history and high Y-STR diversity.

    Science.gov (United States)

    Fadhlaoui-Zid, Karima; Chennakrishnaiah, Shilpa; Zemni, Ramzi; Grinberg, Sagy; Herrera, Rene J; Benammar-Elgaaied, Amel

    2012-12-01

    In the present study, 17 Y-chromosomal STR (Y-STR) loci were typed in 218 unrelated males from Sousse, Central-East Tunisia, to evaluate forensic and population genetic applications of the data. A total of 154 different haplotypes were identified, 127 (82.5%) of which were unique, with the most frequent haplotype occurring in 14 individuals (6.4%). The locus diversity ranged from 0.2050 at DYS392 to 0.8760 at DYS385. The haplotype diversity at the 17-loci resolution was calculated to be 0.9916, while the corresponding values for the extended (11 loci) and minimal (9 loci) haplotypes were estimated at 0.9735 and 0.9710, respectively. Comparison with 29 regional and global populations using correspondence analysis, neighbor joining (NJ) tree, and Rst genetic distance revealed that the Sousse population is highly diverse. This finding is consistent with historical data. Furthermore, the results of this study indicate a distinct genetic substructure among Tunisian populations. In conclusion, the present study demonstrated that the 17 Y-STRs analyzed are highly informative for individual identification, parentage analysis, and population genetic studies. PMID:23160959

  15. A framework for the development of STR genotyping in domestic animal species

    DEFF Research Database (Denmark)

    van Asch, Barbara; Pinheiro, Raquel; Pereira, Rui;

    2010-01-01

    This study reports the methodology used to search, select and characterize STR loci on the canine X chromosome using publicly available genome resources and following the current guidelines for human and non-human forensic testing. After several rounds of selection, 12 X-STR markers were optimized...

  16. Identifying the most likely contributors to a Y-STR mixture using the discrete Laplace method

    DEFF Research Database (Denmark)

    Andersen, Mikkel Meyer; Eriksen, Poul Svante; Mogensen, Helle Smidt;

    2015-01-01

    of the evidence using the likelihood ratio principle when a suspect's Y-STR profile fits into a two person mixture. We used three datasets with between 7 and 21 Y-STR loci: Denmark (n=181), Somalia (n=201) and Germany (n=3443). The Danish dataset with 21 loci was truncated to 15 and 10 loci to examine the effect...

  17. Bengt Strömgren: Growing up with astronomy, 1908-1932

    DEFF Research Database (Denmark)

    Rebsdorf, S.O.

    2003-01-01

    Bengt Strömgren's (1908-1987) early career is examined down to 1932, the year of his first landmark article on astrophysics, in which, continuing the numerical tradition at the Copenhagen Observatory, Strömgren applied the still novel quantum mechanics with great faith in its validity. In additio...

  18. Modellering af strømningsforhold og kanaldannelse i fixed bed koksbed

    DEFF Research Database (Denmark)

    Jensen, Torben Kvist; Henriksen, Ulrik Birk; Gøbel, Benny;

    2003-01-01

    For at kunne undersøge stabiliteten af en koksbed er der blevet udviklet forskellige modeller til beskrivelse af strømningsforholdene i bedden under iltfri forgasning af biomassekoks. Strømningsforholdene er blevet undersøgt på simple modeller og CFD-modeller...

  19. Patterns of Y-STR variation in Italy.

    Science.gov (United States)

    Brisighelli, F; Blanco-Verea, A; Boschi, I; Garagnani, P; Pascali, V L; Carracedo, A; Capelli, C; Salas, A

    2012-12-01

    The 17 Y-chromosomal short tandem repeats (STRs) included in the AmpFlSTR Yfiler Amplification Kit (AB Applied Biosystems) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4.1) were typed in 292 samples from seven Italian regions. Population comparisons with other European samples were undertaken; for this purpose, two databases were collated from the literature: (a) 19 population samples including >2900 Yfiler profiles, and (b) 67 population samples including >15,000 minimum haplotype profiles. A total of 276 different Yfiler haplotypes were observed in Italy, and only one of them was shared among our seven population samples. The overall haplotype diversity (0.9996) was comparable to other European samples. AMOVA indicates that among population variance depends on the amount of Y-STRs used, being higher when using minimal haplotypes. This is probably due to the fact that Yfiler profiles are represented by singleton haplotypes in all the population samples raising the diversity values to the maximum theoretical value. AMOVA results seems to depend even more strongly on the amount of population samples used, the among population variance in Italy ranging from 2.82% to 11.03% (using 15 and 32 Italian populations samples, respectively). Variance is not as strongly stratified geographically within Italy, although it is notorious that latitude is more important than longitude in the distribution of variance. The results also indicated that Italy is less stratified than other European samples. The present study contributes to enrich the Y-chromosome databases regarding high-resolution Y-chromosome data sets and demonstrates that extended Y-STR profiles substantially increases the discriminatory capacity in individual identification for forensic purposes. PMID:22487686

  20. The development of reduced size STR amplicons as tools for analysis of degraded DNA.

    Science.gov (United States)

    Butler, John M; Shen, Yin; McCord, Bruce R

    2003-09-01

    New multiplex PCR sets of commonly used short tandem repeat (STR) markers have been developed to produce PCR products that are reduced in size when compared to standard commercial STR kits. The reduction in size of these amplicons can facilitate the examination and analysis of degraded DNA evidence by improving amplification efficiency. This "miniSTR" approach will permit current forensic practitioners to use STR markers and instrumentation already present in their laboratories and to generate genotyping data that is directly comparable to reference samples and searchable through the FBI's Combined DNA Index System (CODIS) databases. This paper discusses the development of these new primer sets and presents some initial results in the analysis of degraded and aged DNA samples. A method for removal of problematic fluorescent dye artifacts is also described. Comparison studies in over 100 samples have verified that these miniSTR primers can provide fully concordant results to commercial STR kits and can provide improved signal from degraded DNA specimens. These miniplex sets should prove valuable in the analysis of samples where allele dropout and reduced sensitivity of larger STR alleles occurs.

  1. Auslegung, Konstruktion und Fertigung von strömungsmechanischen Funktionsprototypen mittels Rapid Manufacturing

    OpenAIRE

    Martha, Alexander; Sehrt, Jan; Benra, Friedrich-Karl; Köhler, Peter

    2012-01-01

    Bei der Fertigung von Funktionsbauteilen für Strömungsversuche spielt das Design und die Komplexität der Bauteilgeometrie eine wesentliche Rolle. Ziel der interdisziplinären Zusammenarbeit der Lehrstühle Strömungsmaschinen, Rechnereinsatz in der Konstruktion und Fertigungstechnik mit dem Rapid Technology Center (RTC) an der Universität Duisburg-Essen ist es, das Potenzial der additiven Fertigungsverfahren bei der Herstellung von Funktionsprototypen für strömungsmechanische Anwendungen effekti...

  2. Improving DNA data exchange: validation studies on a single 6 dye STR kit with 24 loci.

    Science.gov (United States)

    Martín, Pablo; de Simón, Lourdes Fernández; Luque, Gracia; Farfán, María José; Alonso, Antonio

    2014-11-01

    The idea of developing a new multiplex STR amplification system was conceived in 2011 as an effective way to implement the new European standard set (ESS) of 12 STR markers adopted by The Council of the European Union in 2009 while maintaining an effective compatibility and information exchange with the historical DNA profiles contained in the Spanish national DNA database (around 200,000 DNA profiles) mainly based on the 13 CODIS core STR loci plus D19S433 and D2S1338 markers. With this goal in mind we proposed to test and validate a single STR amplification system for simultaneous analysis of 21 STR markers covering both CODIS and ESS core STR loci plus three additional markers (D19S433, D2S1338, and SE33) also contained in commonly used STR kits and national DNA databases. In 2012, we started the first beta-testing with a 6-dye STR kit prototype containing 24 loci (now known as the GlobalFiler™ PCR Amplification Kit) developed by Life Technologies in response to the CODIS Core Loci Working Group's recommendation to expand the CODIS Core Loci. This prototype included our proposal of 21 autosomal STR markers and two Y-chromosome markers (DYS391 and Y-indel) and maximizes concordance with established databases and previously analyzed samples by maintaining primer sequences of previous Identifiler(®)/NGM SElect™ kits for the 21 STR markers except for TPOX. This paper describes the validation studies conducted with the first commercial available 6-dye STR kit for casework using a 3500 genetic analyzer for fragment detection that included the analysis of the following parameters and aspects: analytical threshold, sensitivity & stochastic threshold, heterozygous balance, stutter threshold, precision and accuracy, repeatability and reproducibility, genotype concordance, DNA mixtures, species specificity, and stability studies with case type samples. The studies demonstrated that the GlobalFiler™ system provided equivalent overall performance to previous forensic

  3. Verwendung von Leistungs-Lumineszenzdioden (LED) in der Strömungsmesstechnik

    OpenAIRE

    Mößner, Steffen

    2009-01-01

    Die nachfolgend beschriebenen Untersuchungen dokumentieren die Leistung von Hochleistungs-LEDs, die für kurze Zeit mit hohen Strömen übersteuert werden. Dies führt zu kurzen Lichtpulsen, welche über das Potential verfügen, laserbasierte Lichtquellen in der Strömungsdiagnostik und verwandten bildgebenden diagnostischen Methoden zu ersetzen. Die kurze Pulsdauer erlaubt es, die untersuchten LEDs mit Strömen zu betreiben, die signifikant oberhalb der bekannten Zerstörschwellen liegen und somit te...

  4. 降落PCR扩增人类常染色体STR D15S128%Touchdown PCR for Amplification of Human Autosomal STR D15S128

    Institute of Scientific and Technical Information of China (English)

    张艳萍; 郭大玮; 马莉莉

    2010-01-01

    目的 优化PCR程序,尝试用降落PCR扩增人类常染色体STR D15S128.方法 用普通PCR和降落PCR扩增人类常染色体STR D15S128.结果 普通PCR扩增产物有非特异带,降落PCR无非特异带.结论 降落PCR简化了普通PCB中摸索最适退火温度的过程,可以一步找到人类常染色体STR D15S128的退火温度,是一种高效率的PCR方法.

  5. STR polymorphisms of the Henan population and investigation of the Central Plains Han origin of Chaoshanese.

    Science.gov (United States)

    Xu, Li-Na; Hu, Sheng-Ping; Feng, Guo-Ying

    2009-08-01

    Allele frequencies for 15 short tandem repeat (STR) loci were obtained from a Chinese Han population in Henan province of middle China. No deviation from Hardy-Weinberg equilibrium was observed for the STR loci except for D3S1358. The 15 STR loci are potentially useful for paternity testing and forensic casework in the Henan population. A phylogenetic tree based on CODIS STR allele frequencies of 25 Han populations revealed noticeable but far less clear distinctions between southern and northern Chinese populations; the Henan Han population was located at an intermediate position between south and north Chinese Han populations, relatively closer to Chaoshan and Minnan Han. Moreover, admixture analysis showed a large proportion of Central Plains Han origin in Chaoshanese and Minnanese. Admixture and phylogenetic analysis also reflected the genetic similarity shared by these two groups.

  6. Analysis of matches and partial-matches in a Danish STR data set

    DEFF Research Database (Denmark)

    Tvedebrink, Torben; Eriksen, Poul Svante; Curan, James Michael;

    2012-01-01

    Over the recent years, the national databases of STR profiles have grown in size due to the success of forensic DNA analysis in solving crimes. The accumulation of DNA profiles implies that the probability of a random match or near match of two randomly selected DNA profiles in the database...... increases. We analysed 53,295 STR profiles from individuals investigated in relation to crime case investigations at the Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark. Incomplete STR profiles (437 circa 0.8% of the total), 48 redundant STR profiles from...... other, i.e. 1.3 × 109 comparisons. With these large number of comparisons, it is likely to observe DNA profiles that coincide on many loci, which has concerned some commentators and raised questions about “overstating” the power of DNA evidence. We used the method of Weir [11] and [12] and Curran et al...

  7. Genetic analysis of two STR loci (VWA and TPOX in the Iranian province of Khuzestan

    Directory of Open Access Journals (Sweden)

    Ali Mohammad Foroughmand

    2014-08-01

    Conclusion: The examined STR loci in this study have proven a relatively high genetic variation in the Iranian population. The data could be used for construction of a forensic genetic database for the Iranian population.

  8. CRED Subsurface Temperature Recorder (STR) at selected locations in the Pacific

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. STR data for PowerPlex 16 System from Neuquen population, SW Argentina.

    Science.gov (United States)

    Toscanini, Ulises; Berardi, Gabriela; Raimondi, Eduardo

    2003-07-01

    Allele frequencies for the 15 autosomic STR loci included in the PowerPlex 16 System kit (Promega) were estimated from a sample of 111 unrelated individuals living in Neuquen province, southwest of Argentina. Population showed to be in HWE.

  10. The evolution of DNA databases--recommendations for new European STR loci

    DEFF Research Database (Denmark)

    Gill, Peter; Fereday, Lyn; Morling, Niels;

    2005-01-01

    was increased when small amplicons (mini-STRs) were analysed. Consequently, it was recommended that existing multiplexes are re-engineered to enable small amplicon detection, and that three new mini-STR loci with alleles

  11. Comparison of STR polymorphism among a Kirgiz ethnic group from Sinkiang and other groups

    Institute of Scientific and Technical Information of China (English)

    Gao Shuhui; Li Shengbin

    2007-01-01

    Objective To study the genetic relationship between Kirgiz individuals living in Sinkiang China and analyze the difference among Kirgiz and the other population with STR polymorphisms. Methods PCR amplification was performed using PE9700, the PCR products were typed by automated sequencer and genescan. Results A database of nine STR loci of Kirgiz was established. It shows there are at least 73 STR alleles and 191 genotypes in Kirgiz. Genotype frequencies distribution showed no deviation from Hardy-Weinberg equilibrium by χ2-test. Kirgiz was compared with the other Chinese ethnic groups, then the American Black and the White. Conclusion These results suggested that the nine STR loci and Amelogenin locus were very useful in human identification, biological archaeology and gene resource studies.

  12. Haplotype diversity of 17 Y-chromosomal STR loci in the Bangladeshi population.

    Science.gov (United States)

    Alam, Shafiul; Ali, Md Eunus; Ferdous, Ahmad; Hossain, Tania; Hasan, Md Mahamud; Akhteruzzaman, Sharif

    2010-02-01

    Haplotype and allele frequencies of 17 Y-chromosomal STR loci were determined in 216 unrelated Bangladeshi males. AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) was used to type the following Y-STR markers: DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439, DYS437, DYS448, DYS458, DYS456, DYS635, and Y-GATA-H4. A total of 211 haplotypes for the 17 Y-STR markers were detected and, of these, 206 haplotypes were unique. The haplotype diversity was 0.9998, indicating a high potential for differentiating between male individuals in this population. Comparison analysis via Analysis of Molecular Variance (AMOVA) and construction of Neighbor Joining Tree revealed a close association of Bangladeshi population with Indian Gaddi and Southern Indian populations. PMID:20129457

  13. Evaluation of the Early Access STR Kit v1 on the Ion Torrent PGM™ platform.

    Science.gov (United States)

    Guo, Fei; Zhou, Yishu; Liu, Feng; Yu, Jiao; Song, He; Shen, Hongying; Zhao, Bin; Jia, Fei; Hou, Guangwei; Jiang, Xianhua

    2016-07-01

    The Early Access STR Kit v1 is designed to detect 25-plex loci with next generation sequencing (NGS) technology on the Ion Torrent PGM™ platform, including 16 of 20 expanded Combined DNA Index System (CODIS) core loci (CSF1PO, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D13S317, D16S539, D19S433, D21S11, TH01, TPOX and vWA), 8 non-CODIS core loci (D1S1677, D2S1776, D4S2408, D5S2500.AC008791, D6S1043, D6S474, D9S2157 and D14S1434) and Amelogenin. In this study, we compared the Early Access STR Kit v1 with the Ion Torrent™ HID STR 10-plex to find out its improvements and explored an appropriate analytical threshold to enhance the performance. In addition, seven experiments were conducted to evaluate the Early Access STR Kit v1 such as studies of repeatability, concordance, sensitivity, mixtures, degraded samples, case-type samples and pedigrees. Other than a little discordance (0.95%) with CE-STR results observed at D21S11, NGS-STR results correctly reflected the sample being tested. Repeatable results were obtained from both initial PCRs and emPCRs aside from a few variations of allele coverage. Full profiles could be obtained from 100pg input DNA and >48.84% profiles from 10pg input DNA. Mixtures were easily detected at 9:1 and 1:9 ratios. This system could be adapted to case-type samples and degraded samples. As a whole, the Early Access STR Kit v1 is a robust, reliable and reproducible assay for NGS-STR typing and a potential tool for human identification.

  14. Characterization of copy number variation in genomic regions containing STR loci using array comparative genomic hybridization.

    Science.gov (United States)

    Repnikova, Elena A; Rosenfeld, Jill A; Bailes, Andrea; Weber, Cecilia; Erdman, Linda; McKinney, Aimee; Ramsey, Sarah; Hashimoto, Sayaka; Lamb Thrush, Devon; Astbury, Caroline; Reshmi, Shalini C; Shaffer, Lisa G; Gastier-Foster, Julie M; Pyatt, Robert E

    2013-09-01

    Short tandem repeat (STR) loci are commonly used in forensic casework, familial analysis for human identification, and for monitoring hematopoietic cell engraftment after bone marrow transplant. Unexpected genetic variation leading to sequence and length differences in STR loci can complicate STR typing, and presents challenges in casework interpretation. Copy number variation (CNV) is a relatively recently identified form of genetic variation consisting of genomic regions present at variable copy numbers within an individual compared to a reference genome. Large scale population studies have demonstrated that likely all individuals carry multiple regions with CNV of 1kb in size or greater in their genome. To date, no study correlating genomic regions containing STR loci with CNV has been conducted. In this study, we analyzed results from 32,850 samples sent for clinical array comparative genomic hybridization (CGH) analysis for the presence of CNV at regions containing the 13 CODIS (Combined DNA Index System) STR, and the Amelogenin X (AMELX) and Amelogenin Y (AMELY) loci. Thirty-two individuals with CNV involving STR loci on chromosomes 2, 4, 7, 11, 12, 13, 16, and 21, and twelve with CNV involving the AMELX/AMELY loci were identified. These results were correlated with data from publicly available databases housing information on CNV identified in normal populations and additional clinical cases. These collective results demonstrate the presence of CNV in regions containing 9 of the 13 CODIS STR and AMELX/Y loci. Further characterization of STR profiles within regions of CNV, additional cataloging of these variants in multiple populations, and contributing such examples to the public domain will provide valuable information for reliable use of these loci.

  15. Evaluation of the Early Access STR Kit v1 on the Ion Torrent PGM™ platform.

    Science.gov (United States)

    Guo, Fei; Zhou, Yishu; Liu, Feng; Yu, Jiao; Song, He; Shen, Hongying; Zhao, Bin; Jia, Fei; Hou, Guangwei; Jiang, Xianhua

    2016-07-01

    The Early Access STR Kit v1 is designed to detect 25-plex loci with next generation sequencing (NGS) technology on the Ion Torrent PGM™ platform, including 16 of 20 expanded Combined DNA Index System (CODIS) core loci (CSF1PO, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D13S317, D16S539, D19S433, D21S11, TH01, TPOX and vWA), 8 non-CODIS core loci (D1S1677, D2S1776, D4S2408, D5S2500.AC008791, D6S1043, D6S474, D9S2157 and D14S1434) and Amelogenin. In this study, we compared the Early Access STR Kit v1 with the Ion Torrent™ HID STR 10-plex to find out its improvements and explored an appropriate analytical threshold to enhance the performance. In addition, seven experiments were conducted to evaluate the Early Access STR Kit v1 such as studies of repeatability, concordance, sensitivity, mixtures, degraded samples, case-type samples and pedigrees. Other than a little discordance (0.95%) with CE-STR results observed at D21S11, NGS-STR results correctly reflected the sample being tested. Repeatable results were obtained from both initial PCRs and emPCRs aside from a few variations of allele coverage. Full profiles could be obtained from 100pg input DNA and >48.84% profiles from 10pg input DNA. Mixtures were easily detected at 9:1 and 1:9 ratios. This system could be adapted to case-type samples and degraded samples. As a whole, the Early Access STR Kit v1 is a robust, reliable and reproducible assay for NGS-STR typing and a potential tool for human identification. PMID:27082757

  16. Analysis of matches and partial-matches in a Danish STR data set.

    Science.gov (United States)

    Tvedebrink, Torben; Eriksen, Poul Svante; Curran, James Michael; Mogensen, Helle Smidt; Morling, Niels

    2012-05-01

    Over the recent years, the national databases of STR profiles have grown in size due to the success of forensic DNA analysis in solving crimes. The accumulation of DNA profiles implies that the probability of a random match or near match of two randomly selected DNA profiles in the database increases. We analysed 53,295 STR profiles from individuals investigated in relation to crime case investigations at the Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark. Incomplete STR profiles (437 circa 0.8% of the total), 48 redundant STR profiles from monozygotic twins (0.09%), 6 redundant STR profiles of unknown cause and 1283 STR profiles from repeated testing of individuals were removed leaving 51,517 complete 10 locus STR profiles for analysis. The number corresponds to approximately 1% of the Danish population. We compared all STR profiles to each other, i.e. 1.3×10(9) comparisons. With these large number of comparisons, it is likely to observe DNA profiles that coincide on many loci, which has concerned some commentators and raised questions about "overstating" the power of DNA evidence. We used the method of Weir [11,12] and Curran et al. [3] to compare the observed and expected number of matches and near matches in the data set. We extended the methods by computing the covariance matrix of the summary statistic and used it for the estimation of the identical-by-descent parameter, θ. The analysis demonstrated a number of close relatives in the Danish data set and substructure. The main contribution to the substructure comes from close relatives. An overall θ-value of 1% compensated for the observed substructure, when close familial relationships were accounted for.

  17. Y-STR markers from Ladakh in the Himalayas.

    Science.gov (United States)

    Perez-Benedico, David; Chennakrishnaiah, Shilpa; Gayden, Tenzin; Rowold, Diane J; Garcia-Bertrand, Ralph; Herrera, Rene J

    2016-07-01

    A total of 223 samples from the general population of Ladakh in Northwestern India were amplified at 17 Y-STR loci using the AmpFlSTR® Yfiler™ system. The DNA profiles generated were employed to generate allelic frequencies, gene diversity, haplotype diversity and discrimination capacity values as well as number of different haplotypes, fraction of unique haplotypes and Rst pair wise genetic distances. Multidimensional Scaling (MDS) and Correspondence Analysis (CA) were performed with the Rst values and allelic frequencies, respectively. The 17-loci discrimination capacity of Ladakh was found to be 0.8093. Eleven out of the 16 loci have diversity values greater than 0.6, and 13 loci possess values greater than 0.5. Ladakh exhibits no significant genetic difference to seven of the 15 reference forensic databases after Bonferroni correction, three of which are located in South Central Asian and four are from the Himalayan region. Rst genetic distance values before and after Bonferroni corrections illustrate the capacity of the Yfiler system to discriminate among Himalayan populations. The intermediate position of the Ladakh population in the MDS and CA plots likely reflects genetic flow and admixture with neighboring populations. In addition, the longitudinal partition of populations in the MDS and CA plots likely reflect human dispersals such as the silk road migrations. PMID:27497330

  18. Identification and characterization of variant alleles at CODIS STR loci.

    Science.gov (United States)

    Allor, Catherine; Einum, David D; Scarpetta, Marco

    2005-09-01

    Short tandem repeat (STR) profiles from 32,671 individuals generated by the ABI Profiler Plus and Cofiler systems were screened for variant alleles not represented within manufacturer-provided allelic ladders. A total of 85 distinct variants were identified at 12 of the 13 CODIS loci, most of which involve a truncated tetranucleotide repeat unit. Twelve novel alleles, identified at D3S1358, FGA, D18S51, D5S818, D7S820 and TPOX, were confirmed by nucleotide sequence analysis and include both insertions and deletions involving the repeat units themselves as well as DNA flanking the repeat regions. Population genetic data were collected for all variants and frequencies range from 0.0003 (many single observations) to 0.0042 (D7S820 '10.3' in North American Hispanics). In total, the variant alleles identified in this study are carried by 1.6% of the estimated 1 million individuals tested annually in the U.S. for the purposes of parentage resolution. A paternity case involving a recombination event of paternal origin is presented and demonstrates how variant alleles can significantly strengthen the genetic evidence in troublesome cases. In such instances, increased costs and turnaround time associated with additional testing may be eliminated.

  19. Polymorphisms at 17 Y-STR loci in Botswana populations.

    Science.gov (United States)

    Tau, Tiroyamodimo; Davison, Sean; D'Amato, María Eugenia

    2015-07-01

    Seventeen Y-chromosomal short tandem repeats (YSTRs)-DYS19, DYS389I, DYS389II, DYS385a/b, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4-were analyzed in 252 unrelated male individuals from Botswana. A total of 238 unique haplotypes were identified. The discrimination capacity (DC) was 0.9444 whereas the haplotype diversity (HD) was 0.9990. A database search of the 238 unique haplotypes in the Y chromosome haplogroup database (YHRD) yielded three African American, six Sub-Saharan African, and two admixed South American matches. Five additional African-American matches were detected in the Applied Biosystems Y-STR database. RST, multi-dimensional scaling (MDS) and AMOVA were used to investigate population differentiation in Sub-Saharan Africa and in Botswana. The populations in Sub-Saharan Africa were found to be heterogeneous, with Botswana showing significant differences from its neighbors. No geographic regional or ethnic differentiation was observed within Botswana. Regional and ethnic variation can be useful in forensic working hypotheses. PMID:25817844

  20. Polymorphism analysis of 15 STR loci in a large sample of Guangdong (Southern China) Han population.

    Science.gov (United States)

    Chen, Ling; Lu, Huijie; Qiu, Pingming; Yang, Xingyi; Liu, Chao

    2015-11-01

    AmpFℓSTR Sinofiler PCR Amplification Kit is specially developed for Chinese forensic laboratories, but there are little population-genetic data about this kit for Southern China. This kit contains 15 STR loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, D6S1043, D12S391, D5S818 and FGA. We have conducted genotyping experiments on the 15 STR loci in 5234 unrelated individuals from Guangdong (Southern China). We observed a total of 243 alleles in the group with the allelic frequency values ranging from less than 0.0001 to 0.3686. Our statistic analysis indicates that the 15 STR loci conform to the Hardy-Weinberg's equilibrium (p>0.05). The highest polymorphism was found at D6S1043 locus and the lowest was found at D3S1358. The combined power of discrimination reached 0.99999999999999999977431 and the combined probability of paternity exclusion reached 0.999999721 for 15 STR loci. Guangdong Han population had significant differences compared with Shaanxi, Shandong and Henan province of Northern China. A Neighbor-joining tree indicates that the Guangdong Han has a close genetic relationship with the Yunnan population. Significant differences were found between Guangdong Han population and other reported populations (Japanese, Philippine, African American, Caucasian, Hispanic and Western Romanian) at 2-11 STR loci. The results may provide useful information for forensic sciences and population genetics studies. The present findings indicate that all the 15 STR loci are highly genetically polymorphic in the Han population of Guangdong.

  1. 原子荧光光谱法测定不同产地郁金中砷和硒含量%Determination of Trace Arsenic and Selenium in Curcuma aromatica with Microwave Decomposition-Atomic Fluorescence Spectrometry

    Institute of Scientific and Technical Information of China (English)

    刘艳清; 汪洪武; 蔡璇

    2014-01-01

    After microwave decomposition, the contents of arsenic and selenium in eleven Curcuma aromatica samples were determined by hydride generation atomic fluorescence spectrometry, and plant standard reference materials were used to verify the accuracy and the precision of the analytical method. The experimental conditions were optimized. Under the optimum conditions, there was a good linear relationship between the fluorescence intensity and the contents of arsenic and selenium in the range of 0-20 μg/L with a correlation coefficient of 0.999 8 and 0.999 3, respectively. The recovery rate of arsenic and selenium were in the range of 97.15%-102.87%and 98.35%-101.76%. The results indicated that the contents of arsenic and selenium in different habits of Curcuma aromatica were significant deviation.%应用AFS-930型双道原子荧光光度计,采用湿法消解方式,在选定仪器工作条件下,建立了原子荧光光谱法测定郁金中砷和硒。以工作曲线法测定了不同产地郁金中砷和硒的含量,并用植物标准参考物质评价了分析方法的准确度。在0~20.0μg/L范围内As及Se的线性良好,线性回归方程为:As:If=48.9908 c+55.2433(r=0.9998);Se:If=49.3717 c+46.5837(r=0.9993),砷和硒的回收率分别为97.15%~102.87%和98.35%~101.76%。研究结果表明不同产地郁金中As和Se的含量存在较大差异。

  2. Second-generation sequencing of forensic STRs using the Ion Torrent™ HID STR 10-plex and the Ion PGM™

    DEFF Research Database (Denmark)

    Fordyce, Sarah L; Mogensen, Helle Smidt; Børsting, Claus;

    2015-01-01

    by Ion Torrent™ software. Hence, the combination of the STR 10-plex and the Ion PGM™ represents the first fully integrated SGS STR typing solution from PCR to data analysis. In this study, four experiments were performed to evaluate the alpha-version of the STR 10-plex: (1) typing of control samples; (2...... crime and identification cases, in which only partial profiles were obtained with PCR-CE assays. In conclusion, the Ion Torrent™ HID STR 10-plex panel offers an all-in-one solution from amplification of STRs and amelogenin, and sequencing to data analysis....

  3. Y-STR genetic screening by high-resolution melting analysis.

    Science.gov (United States)

    Deng, J Q; Liu, B Q; Wang, Y; Liu, W; Cai, J F; Long, R; Li, W H

    2016-01-01

    Currently, the widely used automated capillary electrophoresis-based short tandem repeat (STR) genotyping method for genetic screening in forensic practice is laborious, time-consuming, expensive, and technically challenging in some cases. Thus, new molecular-based strategies for conclusively identifying forensically relevant biological evidence are required. Here, we used high-resolution melting analysis (HRM) for Y-chromosome STR genotyping for forensic genetic screening. The reproducibility of the melting profile over dilution, sensitivity, discrimination power, and other factors was preliminarily studied in 10 Y-STR loci. The results showed that HRM-based approaches revealed more genotypes (compared to capillary electrophoresis), showed higher uniformity in replicate tests and diluted samples, and enabled successful detection of DNA at concentrations as low as 0.25 ng. For mixed samples, the melting curve profiles discriminated between mixed samples based on reference samples with high efficiency. The triplex Y-chromosome STR HRM assay was performed and provided a foundation for further studies such as a multiplex HRM assay. The HRM approach is a one-step application and the entire procedure can be completed within 2 h at a low cost. In conclusion, our findings demonstrate that the HRM-based Y-STR assay is a useful screening tool that can be used in forensic practice. PMID:26909950

  4. Population genetic data for 17 Y STR markers from Benghazi (East Libya).

    Science.gov (United States)

    Elmrghni, Samir; Coulson-Thomas, Yvette M; Kaddura, Mahmoud; Dixon, Ron A; Williams, D Ross

    2012-03-01

    The seventeen Y-STR loci included in the AmpFℓSTR(®) Yfiler™ PCR Amplification kit (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS438, DYS439, DYS437, DYS448, DYS458, DYS456, DYS635, and Y-GATA-H4) were used to type a sample population of 238 males from eastern Libya (Benghazi region). Of 238 observed haplotypes, 214 were unique (90%) and 24 (10%) were found more than once. The 17 loci gave a discriminating power of 0.999. DYS458 showed the highest diversity as a single-locus marker (0.73). Allelic frequencies and gene diversities for each Y-STR locus were determined. The high haplotype diversity and discrimination capacity (0.996) demonstrate the utility of these loci for human identification in forensic applications. Comparative analysis with Y-STR datasets of relevant populations and submission of the haplotypes to the Y-STR Haplotype Reference Database (YHRD) was undertaken. PMID:21640679

  5. Y-STR analysis of digital and/or penile penetration cases with no detected spermatozoa.

    Science.gov (United States)

    McDonald, Andrew; Jones, Emma; Lewis, Jennie; O'Rourke, Paula

    2015-03-01

    This forensic casework trial involved Yfiler(®) testing samples from 47 digital and/or penile penetration cases where the medical examination had occurred within 48h of the alleged incident and no spermatozoa had been detected following Sperm Elution(©). 30% of these cases yielded at least one Y-STR profile comprising three or more alleles per profile and 21% yielded at least one Y-STR profile of ten or more alleles per profile. This trial further investigated the persistence of male DNA in different case types, the location of samples submitted for testing and whether samples from different locations benefit from being combined prior to testing. The data supports the use of Y-STR profiling to provide scientific evidence to investigate whether the alleged sexual activity had occurred as well as to obtain probative evidence in spermatozoa negative penetration cases. PMID:25458926

  6. I-DNASE21 system: development and SWGDAM validation of a new STR 21-plex reaction.

    Science.gov (United States)

    Aznar, Jose María; Celorrio, David; Odriozola, Adrian; Köhnemann, Stephan; Bravo, María Luisa; Builes, Juan Jose; Pfeiffer, Heidi; Herrera, René J; de Pancorbo, Marian M

    2014-01-01

    I-DNASE21 is a new STR multiplex system that amplifies 21 STR autosomal loci, plus the amelogenin locus in one reaction. This system has been designed to analyze all the STR loci included in the Combined DNA Index System (CODIS), Interpol Standard Set of Loci (ISSL), Extended European Standard Set (ESS-Extended), UK National Criminal Intelligence DNA Database (NDNAD) and German Core loci (GCL). This manuscript presents the validation of the I-DNASE21 system according to the revised guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM). The results of this validation, added to the extremely high discriminatory power showed, suggest that I-DNASE21 could be a potentially helpful tool for identification and kinship determination even in complex paternity cases.

  7. Introduction of the Python script STRinNGS for analysis of STR regions in FASTQ or BAM files and expansion of the Danish STR sequence database to 11 STRs

    DEFF Research Database (Denmark)

    Friis, Susanne L; Buchard, Anders; Rockenbauer, Eszter;

    2016-01-01

    This work introduces the in-house developed Python application STRinNGS for analysis of STR sequence elements in BAM or FASTQ files. STRinNGS identifies sequence reads with STR loci by their flanking sequences, it analyses the STR sequence and the flanking regions, and generates a report...... with the assigned SNP-STR alleles. The main output file from STRinNGS contains all sequences with read counts above 1% of the total number of reads per locus. STR sequences are automatically named according to the nomenclature used previously and according to the repeat unit definitions in STRBase (http......01, TPOX, vWA), and Amelogenin was analysed with STRinNGS. Sequencing uncovered five common SNPs near four STRs and revealed 20 new alleles in the 207 Danes. Three short homopolymers in the D8S1179 flanking regions caused frequent sequencing errors. In 29 of 3726 allele calls (0.8%), sequences...

  8. Ein konservatives MPV-Verfahren zur Simulation der Strömungen in allen Machzahlbereichen

    OpenAIRE

    Park, Jea-Ho

    2003-01-01

    Zur Simulation sowohl inkompressibler als auch kompressibler Strömungen wird ein neues numerisches Verfahren eingeführt. Dabei wird zunächst die konservative Form der Navier-Stokes-Gleichungen aufgestellt, da sie das numerische Erhaltungsprinzip für Strömungen höherer Machzahl gewährleistet. Auf den Druck wird noch der sogenannte MPV-Ansatz angewandt. Dies ist eine Art Druckzerlegungsmethode. Damit kann die beim inkompressiblen Grenzfall auftretende Singularität beseitigt werden. Eigentlich b...

  9. Estimating the probability of allelic drop-out of STR alleles in forensic genetics

    DEFF Research Database (Denmark)

    Tvedebrink, Torben; Eriksen, Poul Svante; Mogensen, Helle Smidt;

    2009-01-01

    In crime cases with available DNA evidence, the amount of DNA is often sparse due to the setting of the crime. In such cases, allelic drop-out of one or more true alleles in STR typing is possible. We present a statistical model for estimating the per locus and overall probability of allelic drop......-out using the results of all STR loci in the case sample as reference. The methodology of logistic regression is appropriate for this analysis, and we demonstrate how to incorporate this in a forensic genetic framework....

  10. Wärme- und Strömungssimulation in der Produktentwicklung

    OpenAIRE

    Klett, Sven

    2010-01-01

    Vortrag über die Vorteile konstruktionsbegleitender Wärmesimulation direkt durch den Konstrukteur am nativen CAD-System. Die Elinter AG als Spezialist für Wärmesimulationen und Strömungssimulationen empfiehlt FloEFD zusammen mit Pro/ENGINEER Wildfire. Schnelle thermische Bewertung von Konstruktionsvorschlägen und Designvarianten direkt durch den Konstrukteur. Die frühzeitige Wärmesimulation bzw. Strömungssimulation direkt im Desginprozess ist mit minimalem Aufwand möglich. Simulatione...

  11. Grå strækninger i det åbne land

    DEFF Research Database (Denmark)

    Sørensen, Michael

    Det grå strækningsarbejde er i de sidste 10 år blevet en større og større del af de danske vejbestyrelsers stedbundne trafiksikkerhedsarbejde. Imidlertid er der hverken blevet formuleret en præcis og brugbar definition af begrebet, formuleret en overordnet filosofi for arbejdet eller blevet udvik...

  12. Analysis of 36 Y-STR marker units including a concordance study among 2085 Dutch males

    NARCIS (Netherlands)

    A.A. Westen (Antoinette); T. Kraaijenbrink (Thirsa); L. Clarisse (Lindy); L.J.W. Grol (Laurens J.W.); P. Willemse (Patricia); S.B. Zuniga (Sofia); E.A. Robles De Medina (Elizaveta); R. Schouten (Ron); K. van der Gaag (Kristiaan); J.M. Weiler; A.J. Kal (Arnoud J.); M.H. Kayser (Manfred); T. Sijen (Titia); P. de Knijff (Peter)

    2015-01-01

    textabstractThe genotypes of 36 Y-chromosomal short tandem repeat (Y-STR) marker units were analysed in a Dutch population sample of 2085 males. Profiling results were compared for several partially overlapping kits, i.e. PowerPlex Y, Yfiler, PowerPlex Y23, and two in-house designed multiplexes with

  13. Forensic Spanish allele and haplotype database for a 17 X-STR panel.

    Science.gov (United States)

    Prieto-Fernández, Endika; Núñez, Carolina; Baeta, Miriam; Jiménez-Moreno, Susana; Martínez-Jarreta, Begoña; de Pancorbo, Marian M

    2016-09-01

    The currently developed 17 X-STR panel (DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS6801, DXS7423, DXS6809, DXS6799, DXS7132, DXS9902, DXS6800, DXS6789, DXS10075, DXS10079, DXS6807, and DXS6803) offers a highly discriminative tool for forensic identification and kinship testing. With the aim of providing a global Spanish population X-STR database, we present haplotype and allele frequencies and parameters of forensic interest for the 17 X-STR panel obtained from 593 unrelated individuals from Alicante, Aragon, the Basque Country, Andalusia, Galicia, Madrid, and Barcelona that represent the most populated regions of the Spanish Peninsular territory. The seven populations were compared to test possible population genetic substructures. The lack of significant differences among the studied Spanish populations supports the use of the allele and haplotype frequency database presented herein as a global Spanish population sample useful for statistical evaluation in forensic casework. After conducting the LD plots derived from HapMap and pairwise linkage disequilibrium tests, DXS7132, DXS10075, and DXS10079 markers were included in a cluster and haplotype frequencies were calculated. The improvement in the forensic parameters for the Spanish population using 17 X-STRs in comparison to the previous 10 X-STR allele frequencies database is also shown. PMID:27388427

  14. StrBioLib: a Java library for development of custom computationalstructural biology applications

    Energy Technology Data Exchange (ETDEWEB)

    Chandonia, John-Marc

    2007-05-14

    Summary: StrBioLib is a library of Java classes useful fordeveloping software for computational structural biology research.StrBioLib contains classes to represent and manipulate proteinstructures, biopolymer sequences, sets of biopolymer sequences, andalignments between biopolymers based on either sequence or structure.Interfaces are provided to interact with commonly used bioinformaticsapplications, including (PSI)-BLAST, MODELLER, MUSCLE, and Primer3, andtools are provided to read and write many file formats used to representbioinformatic data. The library includes a general-purpose neural networkobject with multiple training algorithms, the Hooke and Jeeves nonlinearoptimization algorithm, and tools for efficient C-style string parsingand formatting. StrBioLib is the basis for the Pred2ary secondarystructure prediction program, is used to build the ASTRAL compendium forsequence and structure analysis, and has been extensively tested throughuse in many smaller projects. Examples and documentation are available atthe site below.Availability: StrBioLib may be obtained under the terms ofthe GNU LGPL license from http://strbio.sourceforge.net/Contact:JMChandonia@lbl.gov

  15. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci

    DEFF Research Database (Denmark)

    Purps, Josephine; Siegert, Sabine; Willuweit, Sascha;

    2014-01-01

    In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DY...

  16. Autosomal STR allele frequencies for the CODIS system from a large random population sample in Chile.

    Science.gov (United States)

    Vergara, Ismael A; Villouta, Pamela; Herrera, Sandra; Melo, Francisco

    2012-05-01

    The thirteen autosomal STR loci of the CODIS system were typed from DNA of 732 unrelated male individuals sampled from different locations in Chile. This is the first report of allele frequencies for the thirteen STRs loci defined in the CODIS system from the Chilean population.

  17. Genetic data for the 13 CODIS STR loci in Singapore Indians.

    Science.gov (United States)

    Lim, S E S; Tan-Siew, W F; Syn, C K C; Ang, H C; Chow, S T; Budowle, Bruce

    2005-02-10

    Allele frequencies for the 13 CODIS short tandem repeat (STR) loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 174 unrelated Indians in Singapore.

  18. Molluscan fauna of the lower Gelingseh Beds s. str., Sangkulirang area, Kalimantan Timur (East Borneo)

    NARCIS (Netherlands)

    Beets, C.

    1986-01-01

    A compilation is given of the results of an investigation of all the molluscs collected by L.M.R. Rutten some seventy years ago, at five localities in the type area of the Gelingseh Beds s. str., Late Miocene. Thirteen new species are described, viz, Smaragdia gelingsehensis, Rissoina maduparensis,

  19. GENETIC POLYMORPHISM OF SIX Y CHROMOSOMAL STR IN CHINESE HUI ETHNIC GROUP

    Institute of Scientific and Technical Information of China (English)

    Zhu Bofeng; Lü Guiping; Yao Guifa; Zhu Jun; Dong Hongwang; Sun Qingdong; Huang Lei; Liu Yao

    2005-01-01

    Objective To study genetic polymorphism of 6 Y chromosomal STR in Hui ethnic group living in Ningxia Hui ethnic autonomous region, in order to evaluate their usefulness in forensic science and enrich the Chinese genetic information resources. Methods We investigated 101 unrelated, healthy, male individuals of Hui ethnic group and studied their allelic frequency distribution and haplotype diversity of 6 Y chromosomal STR. Primer for each loci was labeled with the fluorescent by FAM (blue) or TAMRA(yellow). The data of Hui ethnic group were generated co-amplification, GeneScan, genotype, and genetic distribution analysis. Results 31 alleles and 43 phenotype(DYS385) were detected, with the frequencies ranging from 0.0099-0.7129. Out of a total of 101 individuals, 96 showed different haplotypes; 91 were unique; 5 were found 2 times. The haplotype diversity for 6 Y-STR loci was 0.9990. Conclusion The date obtained can be valuable for individual identification, paternity testing in forensic fields and for population genetics because of 6 Y-STR loci high polymorphism.

  20. Cluster analysis of European Y-chromosomal STR haplotypes using the discrete Laplace method

    DEFF Research Database (Denmark)

    Andersen, Mikkel Meyer; Eriksen, Poul Svante; Morling, Niels

    2014-01-01

    method can be used for cluster analysis to further validate the discrete Laplace method. A very important practical fact is that the calculations can be performed on a normal computer. We identified two sub-clusters of the Eastern and Western European Y-STR haplotypes similar to results of previous...

  1. An STR forensic typing system for genetic individualization of domestic cat (Felis catus) samples.

    Science.gov (United States)

    Menotti-Raymond, Marilyn A; David, Victor A; Wachter, Leslie L; Butler, John M; O'Brien, Stephen J

    2005-09-01

    A forensic genotyping panel of 11 tetranucleotide STR loci from the domestic cat was characterized and evaluated for genetic individualization of cat tissues. We first examined 49 candidate STR loci and their frequency assessment in domestic cat populations. The STR loci (3-4 base pair repeat motifs), mapped in the cat genome relative to 579 coding loci and 255 STR loci, are well distributed across the 18 feline autosomes. All loci exhibit Mendelian inheritance in a multi-generation pedigree. Eleven loci that were unlinked and were highly heterozygous in cat breeds were selected for a forensic panel. Heterozygosity values obtained for the independent loci, ranged from 0.60-0.82, while the average cat breed heterozygosity obtained for the 11 locus panel was 0.71 (range of 0.57-0.83). A small sample set of outbred domestic cats displayed a heterozygosity of 0.86 for the 11 locus panel. The power of discrimination of the panel is moderate to high in the cat breeds examined, with an average P(m) of 3.7E-06. The panel shows good potential for genetic individualization within outbred domestic cats with a P(m) of 5.31E-08. A multiplex protocol, designed for the co-amplification of the 11 loci and a gender-identifying locus, is species specific and robust, generating a product profile with as little as 0.125 nanograms of genomic DNA.

  2. Automated analysis of sequence polymorphism in STR alleles by PCR and direct electrospray ionization mass spectrometry.

    Science.gov (United States)

    Planz, John V; Sannes-Lowery, Kristen A; Duncan, David D; Manalili, Sheri; Budowle, Bruce; Chakraborty, Ranajit; Hofstadler, Steven A; Hall, Thomas A

    2012-09-01

    Short tandem repeats (STRs) are the primary genetic markers used for the analysis of biological samples in forensic and human identity testing. The discrimination power of a combination of STRs is sufficient in many human identity testing comparisons unless the evidence is substantially compromised and/or there are insufficient relatives or a potential mutation may have arisen in kinship analyses. An automated STR assay system that is based on electrospray ionization mass spectrometry (ESI-MS) has been developed that can increase the discrimination power of some of the CODIS core STR loci and thus provide more information in typical and challenged samples and cases. Data from the ESI-MS STR system is fully backwards compatible with existing STR typing results generated by capillary electrophoresis. In contrast, however, the ESI-MS analytical system also reveals nucleotide polymorphisms residing within the STR alleles. The presence of these polymorphisms expands the number of alleles at a locus. Population studies were performed on the 13 core CODIS STR loci from African Americans, Caucasians and Hispanics capturing both the length of the allele, as well as nucleotide variations contained within repeat motifs or flanking regions. Such additional polymorphisms were identified in 11 of the 13 loci examined whereby several nominal length alleles were subdivided. A substantial increase in heterozygosity was observed, with close to or greater than 5% of samples analyzed being heterozygous with equal-length alleles in at least one of five of the core CODIS loci. This additional polymorphism increases discrimination power significantly, whereby the seven most polymorphic STR loci have a discrimination power equivalent to the 10 most discriminating of the CODIS core loci. An analysis of substructure among the three population groups revealed a higher θ than would be observed compared with using alleles designated by nominal length, i.e., repeats solely. Two loci, D3S1358

  3. Studies on the Reproductive Biology and Endangerment Mechanism of the Endangered Plant Manglietia aromatica%香木莲有性生殖特性与其濒危机制的研究

    Institute of Scientific and Technical Information of China (English)

    潘跃芝; 梁汉兴; 龚洵

    2003-01-01

    The embryogenesis, pollen germination, floral character and seed physiology of the endangered plant Manglietia aromatica Dandy were investigated. Based on this study, this species has very low seed set rate. The abortion rate of functional megaspores in all the ovules is 27.9%, the egg cell abortion rate of mature embryo sacs is up to 80%, and the germination rate of pollen grains is as low as nearly 0.01%. In addition, the floral structure appears to be another limited factor for the effective pollination of this species. The endangerment mechanism of this species seems to be comprehensive. Human's destroying actions are the direct factors that have made the population degenerate quickly; low reproductive ability and the destroyed environments are the main reasons that prevent the population from renovating and spreading. Therefore, the conservation measures suggested by this study are to research the breed technology, artificial population renovating, in situ conservation, and ex situ conservation.%针对香木莲(Manglietia aromatica Dandy)结籽率低以及野外实生苗稀少的现象,本文研究了香木莲雌配子体发育过程、花粉萌发力、开花生物学特性与种子结构的观察.结果表明,在雌配子体发育阶段存在以下退化现象:1) 从大孢子母细胞开始减数分裂到功能大孢子形成阶段,在此过程中珠心内的功能结构发生退化,功能大孢子未能正常形成,仅残留有退化痕迹,退化率为27.9%;2) 胚囊成熟时,受精前卵器细胞发生退化,退化率为80%.花粉萌发实验结果表明,在人工培养条件下具有正常萌发能力的花粉不足0.01%.这些都是香木莲结籽率低的重要原因.同时,香木莲的开花生物学特性限制了传粉的顺利进行.调查和研究的结果显示,人为的砍伐是造成香木莲种群急剧减少的直接原因;有性生殖障碍和生境破坏是制约香木莲种群更新的最主要因素.由此提出了

  4. Population-Scale Sequencing Data Enable Precise Estimates of Y-STR Mutation Rates.

    Science.gov (United States)

    Willems, Thomas; Gymrek, Melissa; Poznik, G David; Tyler-Smith, Chris; Erlich, Yaniv

    2016-05-01

    Short tandem repeats (STRs) are mutation-prone loci that span nearly 1% of the human genome. Previous studies have estimated the mutation rates of highly polymorphic STRs by using capillary electrophoresis and pedigree-based designs. Although this work has provided insights into the mutational dynamics of highly mutable STRs, the mutation rates of most others remain unknown. Here, we harnessed whole-genome sequencing data to estimate the mutation rates of Y chromosome STRs (Y-STRs) with 2-6 bp repeat units that are accessible to Illumina sequencing. We genotyped 4,500 Y-STRs by using data from the 1000 Genomes Project and the Simons Genome Diversity Project. Next, we developed MUTEA, an algorithm that infers STR mutation rates from population-scale data by using a high-resolution SNP-based phylogeny. After extensive intrinsic and extrinsic validations, we harnessed MUTEA to derive mutation-rate estimates for 702 polymorphic STRs by tracing each locus over 222,000 meioses, resulting in the largest collection of Y-STR mutation rates to date. Using our estimates, we identified determinants of STR mutation rates and built a model to predict rates for STRs across the genome. These predictions indicate that the load of de novo STR mutations is at least 75 mutations per generation, rivaling the load of all other known variant types. Finally, we identified Y-STRs with potential applications in forensics and genetic genealogy, assessed the ability to differentiate between the Y chromosomes of father-son pairs, and imputed Y-STR genotypes.

  5. Direct Y-STR amplification of body fluids deposited on commonly found crime scene substrates.

    Science.gov (United States)

    Dargay, Amanda; Roy, Reena

    2016-04-01

    Body fluids detected on commonly found crime scene substrates require extraction, purification and quantitation of DNA prior to amplification and generation of short tandem repeat (STR) DNA profiles. In this research Y-STR profiles were generated via direct amplification of blood and saliva deposited on 12 different substrates. These included cigarette butts, straws, grass, leaves, woodchips and seven different types of fabric. After depositing either 0.1 μL of blood or 0.5 μL of saliva, each substrate containing the dry body fluid stain was punched using a Harris 1.2 mm micro-punch. Each of these punched substrates, a total of 720 samples, containing minute amount of blood or saliva was either amplified directly without any pre-treatment, or was treated with one of the four washing reagents or buffer. In each of these five experimental groups the substrates containing the body fluid remained in the amplification reagent during the thermal cycling process. Each sample was amplified with the three direct Y-STR amplification kits; AmpFℓSTR(®) Yfiler(®) Direct, Yfiler(®) Plus Amplification Kits and the PowerPlex(®) Y23 System. Complete and concordant Y-STR profiles were successfully obtained from most of these 12 challenging crime scene objects when the stains were analyzed by at least one of the five experimental groups. The reagents and buffer were interchangeable among the three amplification kits, however, pre-treatment with these solutions did not appear to enhance the quality or the number of the full profiles generated with direct amplification. This study demonstrates that blood and saliva deposited on these simulated crime scene objects can be amplified directly.

  6. Strömungs- und Transportvorgänge bei kleinen Reynoldszahlen in gekreuzten Strukturen von Plattenwärmeübertragern

    OpenAIRE

    Schill, Christoph Jörg Günther

    2010-01-01

    Das Ziel dieser Arbeit ist es, die Strömungs- und Transportvorgänge in Plattenwärmeübertragern bei geringen Strömungsgeschwindigkeiten mit viskosen Modellflüssigkeiten zu untersuchen. Es werden in einer systematischen Analyse das Strömungs- und Transportverhalten sinusförmig gewellter Strukturen mit gekreuzter Plattenanordnungen bei geringen Reynoldszahlen zwischen 0,1 < Reh < 10 sowohl experimentell als auch mit Hilfe der Strömungssimulation untersucht. Die Sichtbarmachung der Strömungsvo...

  7. Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y.

    Science.gov (United States)

    Zhou, Huaigu; Bi, Gang; Zhang, Chen; Liu, Yanan; Chen, Ronghua; Li, Fayuan; Mei, Xinglin; Guo, Yulin; Zheng, Weiguo

    2016-09-01

    DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16+10Y (EX16+10Y) and Expressmarker 16+18Y (EX16+18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests. The results demonstrated that EX16+10Y and EX16+18Y were useful tools for rapid criminal investigation. PMID:27243781

  8. Allele frequency of 19 autosomal STR loci in the Bai population from the southwestern region of mainland China.

    Science.gov (United States)

    Li, Yi; Hong, Yine; Li, Xiujiang; Yang, Jinmeng; Li, Lanjiang; Huang, Ying; Wang, Chuanchao; Li, Hui; Xu, Bingying

    2015-10-01

    The aim of this study was to investigate a 19 STR loci database using the Bai population from China. This multiplex amplification kit included 13 CODIS STR markers and six plus STR markers (D19S433, Penta E, D2S1338, Penta D, D6S1043, and D12S391) that were successfully analyzed by using 1158 DNA samples from the Bai population from the southwestern part of mainland China. These results indicate that this multiplex amplification kit may provide significant polymorphic information for kinship testing and relationship investigations.

  9. Developmental validation of a single-tube amplification of the 13 CODIS STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR Identifiler PCR Amplification Kit.

    Science.gov (United States)

    Collins, Patrick J; Hennessy, Lori K; Leibelt, Craig S; Roby, Rhonda K; Reeder, Dennis J; Foxall, Paul A

    2004-11-01

    Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFlSTR kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFlSTR Identifiler PCR Amplification Kit for human identity and parentage testing.

  10. Fetal gender determination through Y-STR analysis of maternal plasma during the third trimester of pregnancy

    Directory of Open Access Journals (Sweden)

    Hanaa M.H. Aal-Hamdan

    2015-01-01

    Conclusion: It is recommended to use Y-STR profiling as an alternative technique for fetal gender determination during the third trimester of pregnancy, in addition to its significance in forensic casework.

  11. Assessment of STR Typing Success Rate in Soft Tissues from Putrefied Bodies Based on a Quantitative Grading System for Putrefaction.

    Science.gov (United States)

    Courts, Cornelius; Sauer, Eva; Hofmann, Yaiza; Madea, Burkhard; Schyma, Christian

    2015-07-01

    To date, there is no systematic investigation of the association of short tandem repeat (STR) typing success rate in soft tissues with different signs of putrefaction. Herein, putrefaction was rated using a newly developed 19-parameter system in soft tissues from a collective of 68 decaying bodies, and DNA yield was determined in 408 samples. DNA integrity was rated using a self-devised pentaplex PCR generating an "integrity score" (Si). STR typing success rate was then assessed for selected cases. DNA yield and Si differed significantly between tissues with kidney on average exhibiting the highest Si values. Statistical analysis revealed that nine parameters were significantly and positively correlated with Si . The observed values for each of these nine parameters were summed up to generate a putrefaction score (Sp) for each sample. Our results show that STR typing success rate can be predicted based on Sp before expensive multiplex STR profiling is performed. PMID:25808732

  12. Emigracja do świata klauzurowego – na podstawie relacji sióstr zakonnych

    OpenAIRE

    Zimnica-Kuzioła, Emila

    2015-01-01

    Celem artykułu jest analiza relacji sióstr zakonnych na temat ich emigracji do świata klauzurowego. Spośród kilkudziesięciu wypowiedzi sióstr (reprezentujących różne polskie zgromadzenia zakonne) na temat ich wyboru drogi życiowej wybrałam najciekawsze i najobszerniejsze. Teksty (łącznie 20) zamieszczone na stronach internetowych zakonów poddałam analizie treści, nakierowanej na wyodrębnienie dominujących wątków tematycznych. Mają one ważny walor ko¬gnitywny, pokazują dynamikę powołania do ży...

  13. An Accelerated Analytical Process for the Development of STR Profiles for Casework Samples.

    Science.gov (United States)

    Laurin, Nancy; Frégeau, Chantal J

    2015-07-01

    Significant efforts are being devoted to the development of methods enabling rapid generation of short tandem repeat (STR) profiles in order to reduce turnaround times for the delivery of human identification results from biological evidence. Some of the proposed solutions are still costly and low throughput. This study describes the optimization of an analytical process enabling the generation of complete STR profiles (single-source or mixed profiles) for human identification in approximately 5 h. This accelerated process uses currently available reagents and standard laboratory equipment. It includes a 30-min lysis step, a 27-min DNA extraction using the Promega Maxwell(®) 16 System, DNA quantification in profiles on the 3500-series Genetic Analyzer. This combination of fast individual steps produces high-quality profiling results and offers a cost-effective alternative approach to rapid DNA analysis. PMID:25782346

  14. Genetic polymorphisms for 17 Y-chromosomal STR haplotypes in Jammu and Kashmir Saraswat Brahmin population.

    Science.gov (United States)

    Yadav, Bhuvnesh; Raina, Anupuma; Dogra, Tirath Das

    2010-09-01

    In this study 17 Y-chromosomal STRs (including DYS19, DYS389I, DS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y GATA H4) were analysed using blood samples of 122 unrelated male individuals belonging to Saraswat Brahmin community from Jammu (ID YP000599) and Kashmir (ID YP000600) region of J&K state of India. The allelic frequency distribution and haplotype diversity of 17 Y-chromosomal STR for both the populations were calculated. In the Kashmiri Saraswat group, a total of 109 haplotypes were identified in 122 individuals, of these haplotypes, 101 were found only once. The gene diversity values of STR loci ranged from 0.4813 (DYS391) to 0.8645 (DYS385a/b) for Jammu & Kashmiri Saraswat Brahmins.

  15. A new autosomal STR nineplex for canine identification and parentage testing

    DEFF Research Database (Denmark)

    van Asch, Barbara; Alves, Cíntia; Gusmão, Leonor;

    2009-01-01

    A single multiplex PCR assay capable of simultaneously amplifying nine canine-specific autosomal STR markers (FH3210, FH3241, FH2004, FH2658, FH4012, REN214L11, FH2010, FH2361 and the newly described C38) was developed for individual identification and parentage testing in domestic dogs. In order...... to increase genotyping efficiency, amplicon sizes were optimized for a 90-350 bp range, with fluorescently labelled primers for use in Applied Biosystems, Inc., platforms. The performance of this new multiplex system was tested in 113 individuals from a case-study population and 12 random dogs from mixed...... in an optimized and well-characterized canine DNA genotyping system that is highly performing and straightforward to integrate and employ routinely. Although this STR multiplex was developed for use and tested in a case-study population, the Portuguese breed Cão de Gado Transmontano, it proved to be useful...

  16. Kritisches Denken aufbereitet. Eine Rezension zu: Belina, Naumann, Strüver "Handbuch kritische Stadtgeographie"

    Directory of Open Access Journals (Sweden)

    Kristine Beurskens

    2015-08-01

    Full Text Available Im Sommer des Jahres 2014 erschien das Handbuch kritische Stadtgeographie. Die Her­aus­geber_innen Bernd Belina, Matthias Naumann und Anke Strüver setzten sich mit dem Werk das Ziel, „Studierenden – nicht nur der Geo­graphie, son­dern auch benachbarter Disziplinen – die reichhaltigen Debat­ten kritischer Stadtgeographie – näher zu bringen.“ (Belina/Naumann/Strüver 2014: 12. Dank ihrer spezifischen Inhalte und besonderen Aufbereitung und Verknüpfung von Wissen hat die Publikation einen Anker und Reflexionspunkt zu fachübergreifenden kritischen Debatten und Ansätzen zum Thema ‚Stadt‘ gesetzt. 

  17. A Y-STR database of Iranian and Azerbaijanian minority populations.

    Science.gov (United States)

    Roewer, Lutz; Willuweit, Sascha; Stoneking, Mark; Nasidze, Ivan

    2009-12-01

    Seventeen Y-chromosomal short tandem repeats (Y-STR) DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4 were studied in five minor linguistic groups from Iran (Arabs, Gilaki, Mazandarani, Bakhtiari and Southern Talysh) and one from Azerbaijan (Northern Talysh) with the goal of constructing of a representative Y-STR database for this region in Southwest Asia. Analysis of Molecular Variance (AMOVA) reveals non-significant or low genetic distances between the Iranian Gilaki, Mazandarani, Bakhtiari and non-Iranian Turkish, Azerbaijanian, Armenian and Kurd populations, but larger genetic distances to both Talysh populations, the Iranian Arabs, Georgian and Kazakh populations. PMID:19948326

  18. Population genetics of 17 Y-STR markers in West Libya (Tripoli region).

    Science.gov (United States)

    Triki-Fendri, Soumaya; Sánchez-Diz, Paula; Rey-González, Danel; Ayadi, Imen; Alfadhli, Suad; Rebai, Ahmed; Carracedo, Ángel

    2013-05-01

    Seventeen Y-chromosomal Short Tandem Repeats (Y-STR) included in the AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4) were genotyped in a population sample of 176 unrelated males from western Libya (Tripoli region). A total of 142 different haplotypes were found, 124 being unique. Haplotype diversity was 0.9950. Both R(ST) pairwise analyses and multidimensional scaling plot show a close genetic relationship between Tripoli and North African populations. PMID:23473875

  19. 17 Y-STR haplotype data for a population sample of Residents in the Basque Country.

    Science.gov (United States)

    Valverde, Laura; Köhnemann, Stephan; Rosique, Melania; Cardoso, Sergio; Zarrabeitia, Maite; Pfeiffer, Heidi; de Pancorbo, Marian M

    2012-07-01

    Non autochthonous population is the most numerous group in the Basque Country. This group is named "Residents" to distinguish them from the "Autochthonous Basque" population. In this work, the 17 Y-STR loci distribution of Resident population was studied in a sample of 197 individuals, who were concretely genotyped for DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS439, DYS438, DYS437, DYS448, DYS456, DYS458, DYS635 and Y GATA H4. Resident population showed a high haplotype diversity and discrimination capacity. The distribution of Y-STRs haplotypes of the Resident population was statistically significant different to the one of the Autochthonous Basque population. The genetic substructure found between Resident and Autochthonous Basque 17 Y-STR haplotype distributions advises for the use of two different databases in the Basque Country, to ensure the most trustworthy frequency estimate in casework. PMID:22342392

  20. Genetic polymorphism of 11 Y-chromosomal STR loci in Yunnan Han Chinese.

    Science.gov (United States)

    Yanmei, Yang; Tao, Gu; Yubao, Zeng; Chunjie, Xiao; Bifeng, Chen; Shi, Luo; Bingying, Xu; Qiang, Jing; Qinyong, Zhuang; Wen, Zhang; Shengjun, Luo; Shengjie, Nie

    2010-02-01

    Allele frequencies and haplotypes of 11 Y-chromosome STR loci, DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385 ab, DYS438, DYS439 and DYS437 were determined in 320 unrelated Yunnan Han Chinese males. A total of 293 haplotypes were identified, of which 268 were unique, 23 were shared in two individuals, and 2 were shared in three individuals. The allele diversity values for each locus ranged from 0.4087 (DYS438) to 0.9701 (DYS385). The allele observed haplotypes diversity value was 0.9994. The combined Y-chromosome STR polymorphisms provide a powerful discrimination tool for routine forensic applications. PMID:20129460

  1. Genetic polymorphisms for 17 Y-chromosomal STR haplotypes in Jammu and Kashmir Saraswat Brahmin population.

    Science.gov (United States)

    Yadav, Bhuvnesh; Raina, Anupuma; Dogra, Tirath Das

    2010-09-01

    In this study 17 Y-chromosomal STRs (including DYS19, DYS389I, DS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y GATA H4) were analysed using blood samples of 122 unrelated male individuals belonging to Saraswat Brahmin community from Jammu (ID YP000599) and Kashmir (ID YP000600) region of J&K state of India. The allelic frequency distribution and haplotype diversity of 17 Y-chromosomal STR for both the populations were calculated. In the Kashmiri Saraswat group, a total of 109 haplotypes were identified in 122 individuals, of these haplotypes, 101 were found only once. The gene diversity values of STR loci ranged from 0.4813 (DYS391) to 0.8645 (DYS385a/b) for Jammu & Kashmiri Saraswat Brahmins. PMID:20621539

  2. Allele frequencies of 14 STR loci in the population of Malta.

    Science.gov (United States)

    Cassar, M; Farrugia, C; Vidal, C

    2008-05-01

    Allele frequencies of 14 STR loci (D13S317, D16S539, D2S1338, vWA, TPOX, D18S51, D5S818, FGA, D8S1179, D21S11, D7S820, CSF1PO, TH01 and D3S1358) observed in the population of Malta are being reported. Polymerase chain reaction (PCR) amplification using the AmpFl STR Identifiler kit was performed in a random sample of 157 subjects (314 chromosomes). Markers D2S1338, D18S51 and FGA had the highest power of discrimination (PD) values while TPOX was the least informative marker. Allele frequencies observed in the Maltese population were also compared with those of other populations from the Mediterranean region, Europe and Africa. Our data is useful for anthropological and other comparative studies of populations and is powerful for forensic and paternity testing in the Maltese islands.

  3. Population genetic data on four STR loci in a Hungarian Romany population.

    Science.gov (United States)

    Füredi, S; Kozma, Z; Woller, J; Pádár, Z; Angyal, M; Bajnóczky, I; Nishi, K

    1998-01-01

    A population study of Hungarian Romanies was carried out on the STR loci HumLPL, HumF13B, HumFES and HumF1301. There was little evidence for association of alleles within/between the four STR systems. Allele frequency distributions were significantly different between the Romany and the previously reported Central Hungarian population databases. Population differentiation was estimated by computing F- and phi-statistics as well as frequency estimate differences of individual phenotypes for these two population samples. The results suggest that the population structure may have an effect on the interpretation of forensic DNA evidence in Hungary. Phylogenetic tree reconstruction with six populations from three major ethnic groups revealed a relatively distant genetic relationship of the Baranya Romanies with other Caucasian populations.

  4. Strömungsverhalten thermoplastischer Schmelzen in klüftigem Festgestein

    OpenAIRE

    Irngartinger, Stefan

    2012-01-01

    Die Dissertation beschäftigt sich mit dem Strömungsverhalten thermoplastischer Schmelzen, wie diese für Abdichtungsinjektionen in Böden und klüftigem Festgestein verwendet werden. Zur Untersuchung des Fließ- und Abkühlverhaltens der Schmelze wurden vor allem Injektionsversuche in einen einzelnen Kluftkanal im Gneis ausgeführt und dabei das Temperaturfeld im Fels und im Injektionsmittel analysiert. Auf Basis der Erkenntnisse wurde ein vereinfachtes, analytisches Berechnungsmodell erstellt, ...

  5. Population genetic data for 15 STR loci (Identifiler kit) in Bolivia.

    Science.gov (United States)

    Rocabado, Omar; Taboada, Patricia; Inda, Francisco Javier; Yurrebaso, Inaki; García, Oscar

    2009-11-01

    Allele frequencies for 15 STR autosomal loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818 and FGA) were obtained from a sample of 200 unrelated individuals from Bolivia, South America.

  6. Strömungsuntersuchungen mittels Laser-Doppler-Anemometrie in Modellen humaner Karotisbifurkationen nach Stentimplantation

    OpenAIRE

    Kleinschmidt, Thomas

    2006-01-01

    Ziel:Die Stentangioplastie nimmt einen immer höheren Stellenwert bei der Therapie der Karotistenose ein. In dieser experimentellen Arbeit wurde der Einfluss verschiedener Stentdesigns auf die Strömungsverhältnisse in der Arteria carotis aus rheologischer Sicht untersucht. Im Blickpunkt stand dabei ein neuer Prototyp eines membranunterstützten Stents. Material und Methoden: In maßstabsgetreue Silikonmodelle der A.carotis wurden drei verschiedene Stentmodelle: ein Stent aus rostfreim Stahl (Wal...

  7. The Origin of Workerless Parasites in Leptothorax (S. Str.) (Hymenoptera: Formicidae)

    OpenAIRE

    Jürgen Heinze

    1995-01-01

    The evolutionary origin of workerless parasitic ants parasitizing colonies of Leptothorax (s.str.) is investigated using data on morphology, chromosome number, and allozyme phenotype of both social parasites and their hosts. Of the three previously proposed pathways, the evolution of workerless parasites from guest ants or slave-makers is unlikely, at least according to a phenogram obtained by UPGMA clustering of Nei's similarities based on seven enzymes, lntraspecific evolution of the worker...

  8. Disentangling multiple stellar populations in globular clusters using the Str\\"omgren system

    DEFF Research Database (Denmark)

    Alonso-García, J.; Catelan, M.; Amigo, P.;

    2012-01-01

    clusters, both in our Galaxy and in others. We have started a series of observations of Galactic globular clusters using the Str\\"omgren photometric system in order to find the signatures of these multiple populations and establish their presence in a more complete sample of globular clusters in the Milky...... Way, and to study their radial distributions and extensions. We present here the first results of our survey....

  9. Topical problems connected with the German act on electricity from renewable energy sources (StrEG)

    International Nuclear Information System (INIS)

    The German act (StrEG) intended to enhance the use of renewable energy sources for electricity generation and to promote the relevant technologies raises some problems in connection with constitutional law that still await judicial review by the German Federal Constitutional Court. In addition, doubts as to the lawfulness of provisions of the act have been emerging in connection with EC laws governing the regime of subsidies and state aid. The article here summarizes the current situation. (orig./CB)

  10. Detection of Y STR markers of male fetal dna in maternal circulation

    OpenAIRE

    Nair Seema; Peter Sam; Pillay V; Remya U; Krishnaprasad R; Rajammal B

    2007-01-01

    Background: Circulating fetal cells and cell free DNA in the maternal blood has been shown to help in prenatal diagnosis of genetic disorders without relying on invasive procedures leading to significant risk of pregnancy loss. Aim: The current study was undertaken to detect the male fetal population using Y STR markers DYS 19, DYS 385 and DYS 392 and also to study the extent of persistence of fetal DNA in the mother following delivery. Materials and Methods: Blinded study was conducted ...

  11. Genetic data for 17 Y-chromosomal STR loci in Macedonians in the Republic of Macedonia.

    Science.gov (United States)

    Jakovski, Zlatko; Nikolova, Ksenija; Jankova-Ajanovska, Renata; Marjanovic, Damir; Pojskic, Naris; Janeska, Biljana

    2011-08-01

    The population data were obtained for the 16 Y chromosomal STR loci included in the AmpFistr(®)Yfiler™ PCR Amplification Kit (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385 a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA H4, DYS437, DYS438, DYS448) in a sample of 262 unrelated men from the Republic of Macedonia. PMID:21549657

  12. [Information behavior of 7 STR loci on chromosome 9p in gene scanning].

    Science.gov (United States)

    Zeng, Zhao-Yang; Xiong, Wei; Xiong, Fang; Shen, Shou-Rong; Li, Xiao-Ling; Li, Wei-Fang; Wang, Rong; Xiao, Bing-Yi; Fan, Song-Qing; Huang, He; Zhou, Ming; Li, Gui-Yuan

    2003-09-01

    To get genotype and allele frequency distributions of seven short tandem repeat (STR) loci of chromosome 9p,D9S288,D9S157,D9S1748,D9S171,D9S161,D9S1817 and D9S1805 in Chinese Han population in Hunan area,blood samples were collected from the random Han individual in Hunan and the whole genomic DNA was extracted.STR loci were amplified by multiplex-PCR technique and genotyped by ABI 377 sequencer.Seventy-five alleles were detected,with frequencies ranging from 0.002 to 0.800,and constituted 243 genotypes. All the seven loci met Hardy-Weinberg equilibrium. The statistical analysis of seven STR loci showed H(heterozygosity) ranging from 0.347 to 0.844,DP(discrimination power) ranging from 0.346 to 0.841,PPE(probabilities of paternity exclusion) ranging from 0.308 to 0.738 and PIC(polymorphic information content) ranging from 0.328 to 0.822. The result indicated that there was a significant difference between Han ethnic group and the white and the black.

  13. Design and validation of a highly discriminatory 10-locus Y-chromosome STR multiplex system

    KAUST Repository

    D'Amato, María Eugenia

    2011-03-01

    The Y-chromosome STRs (short tandem repeat) markers are routinely utilized in the resolution of forensic casework related to sexual assault. For this, the forensic community has adopted a set of eleven (core) Y-STR that is incorporated in all commercial diagnostic systems. Our previous studies of Y-STR polymorphisms in the South African population identified low levels of diversity and discrimination capacity for many commercial marker sets, determining a limited applicability of these systems to the local population groups. To overcome this shortcoming, we designed a Y-STR 10-plex system that shows higher discriminatory capacity (DC) than available commercial systems. The markers were selected from a population group of 283 individuals with African, European and Asian ancestry genotyped at 45 Y-STRs, applying an optimization based selection procedure to achieve the highest possible DC with the minimal number of markers. The 10-plex was satisfactorily subjected to developmental validation tests following the SWGDAM guidelines and shows potential for its application to genealogical and evolutionary studies. © 2010 Elsevier Ireland Ltd.

  14. A forensic perspective on the genetic identification of grapevine (Vitis vinifera L.) varieties using STR markers.

    Science.gov (United States)

    Santos, Sara; Oliveira, Manuela; Amorim, António; van Asch, Barbara

    2014-11-01

    The grapevine (Vitis vinifera subsp. vinifera) is one of the most important agricultural crops worldwide. A long interest in the historical origins of ancient and cultivated current grapevines, as well as the need to establish phylogenetic relationships and parentage, solve homonymies and synonymies, fingerprint cultivars and clones, and assess the authenticity of plants and wines has encouraged the development of genetic identification methods. STR analysis is currently the most commonly used method for these purposes. A large dataset of grapevines genotypes for many cultivars worldwide has been produced in the last decade using a common set of recommended dinucleotide nuclear STRs. This type of marker has been replaced by long core-repeat loci in standardized state-of-the-art human forensic genotyping. The first steps toward harmonized grapevine genotyping have already been taken to bring the genetic identification methods closer to human forensic STR standards by previous authors. In this context, we bring forward a set of basic suggestions that reinforce the need to (i) guarantee trueness-to-type of the sample; (ii) use the long core-repeat markers; (iii) verify the specificity and amplification consistency of PCR primers; (iv) sequence frequent alleles and use these standardized allele ladders; (v) consider mutation rates when evaluating results of STR-based parentage and pedigree analysis; (vi) genotype large and representative samples in order to obtain allele frequency databases; (vii) standardize genotype data by establishing allele nomenclature based on repeat number to facilitate information exchange and data compilation.

  15. Haplotypes for 13 Y-chromosomal STR loci in South Tunisian population (Sfax region).

    Science.gov (United States)

    Ayadi, Imen; Ammar-Keskes, Leila; Rebai, Ahmed

    2006-12-20

    Nine Y-STR loci from the "minimal haplotype" (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393) included in Y-STR Haplotype Reference Databases (YHRD) with 4 additional Y-STRs (DYS436, DYS437, DYS438, DYS439) were analyzed by PCR using duplex and Y-PLEX 12 kit, followed by automatic genotyping in a sample of 105 Tunisian males originating from Sfax region (south Tunisia). Allelic frequencies and gene diversities for each Y-STR locus were determined. The high haplotype diversity (0.9932) and discrimination capacity (0.7714) show the usefulness of these loci for human identification in forensic studies and paternity tests in Tunisia. The most common haplotype was shared by 4.7% (5 individuals) of the sample was only found in samples from the Tunisian population reported in YHRD. One private allele for DYS392 (allele 17) was discovered and duplications were observed for five loci (DYS19, DYS389I, DYS393, DYS437 and DYS439).

  16. Reconstructing recent human phylogenies with forensic STR loci: A statistical approach

    Directory of Open Access Journals (Sweden)

    Khan Faisal

    2005-09-01

    Full Text Available Abstract Background Forensic Short Tandem Repeat (STR loci are effective for the purpose of individual identification, and other forensic applications. Most of these markers have high allelic variability and mutation rate because of which they have limited use in the phylogenetic reconstruction. In the present study, we have carried out a meta-analysis to explore the possibility of using only five STR loci (TPOX, FES, vWA, F13A and Tho1 to carry out phylogenetic assessment based on the allele frequency profile of 20 world population and north Indian Hindus analyzed in the present study. Results Phylogenetic analysis based on two different approaches – genetic distance and maximum likelihood along with statistical bootstrapping procedure involving 1000 replicates was carried out. The ensuing tree topologies and PC plots were further compared with those obtained in earlier phylogenetic investigations. The compiled database of 21 populations got segregated and finely resolved into three basal clusters with very high bootstrap values corresponding to three geo-ethnic groups of African, Orientals, and Caucasians. Conclusion Based on this study we conclude that if appropriate and logistic statistical approaches are followed then even lesser number of forensic STR loci are powerful enough to reconstruct the recent human phylogenies despite of their relatively high mutation rates.

  17. Genetic polymorphism of 13 non-CODIS STR loci in three national populations from China.

    Science.gov (United States)

    Liu, Qiu-Ling; Huang, Kai-Kai; Wu, Ye-Da; Zhao, Hu; Li, Cheng-Tao; Lu, De-Jian

    2014-12-01

    The aim of this study was to investigate a 13 non-CODIS STR loci database using three national populations from China. A new multiplex PCR system that simultaneously amplified 13 loci in the same PCR reaction was developed. This multiplex system included the 13 STR markers (D3S2402, D3S2452, D3S1766, D3S4554, D3S2388, D3S3051, D3S3053, D4S2364, D4S2404, AC001348A, AC001348B, D17S975, and D17S1294), which were successfully analyzed by using 441 DNA samples from three national populations in China (154 Mongol, 177 Kazakh, and 110 Uigur). Allele frequencies and mutation rates of the 13 non-CODIS STR loci were investigated. A total of 4-10 alleles at each locus were observed and altogether 84, 88, and 87 alleles for the all selected loci were found in the Mongol, Kazakh, and Uigur, respectively. Eight mutations were detected from the 13 selected loci in 9880 meioses in kinship cases. These results indicate that this multiplex system may provide significant polymorphic information for kinship testing and relationship investigations.

  18. Detection of Y STR markers of male fetal dna in maternal circulation

    Directory of Open Access Journals (Sweden)

    Nair Seema

    2007-01-01

    Full Text Available Background: Circulating fetal cells and cell free DNA in the maternal blood has been shown to help in prenatal diagnosis of genetic disorders without relying on invasive procedures leading to significant risk of pregnancy loss. Aim: The current study was undertaken to detect the male fetal population using Y STR markers DYS 19, DYS 385 and DYS 392 and also to study the extent of persistence of fetal DNA in the mother following delivery. Materials and Methods: Blinded study was conducted on 50 mothers delivering male and female babies. Cellular and cell free DNA was extracted from maternal and fetal cord blood and amplified for Y STR markers by PCR. Results: The amplification sensitivity of Y specific STR, DYS19 was 100% (22/22 in the male fetal DNA samples. The incidence of other STRs, i.e., DYS385 and DYS392 were 91% (20/22 each. Analysis of results revealed that thirteen of the twenty six women had detectable male fetal DNA at the time of delivery. However fetal DNA was not detectable twenty four hours after delivery. Conclusion: Preliminary results show that the separation of fetal cell-free DNA in the maternal circulation is a good low-cost approach for the future development of novel strategies to provide non-invasive techniques for early prenatal diagnosis.

  19. ANALYSIS ON GENETIC POLYMORPHISM OF 6 STR LOCI ON CHROMOSOME 12 IN CHINESE HAN POPULATION

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To analyze the genetic polymorphism of 6 STR loci (D12S358, D12S1675, D12S1663, D12S1697, D12S1725 and D12S1613) on chromosome 12 in Chinese Han population. Methods EDTA-blood specimens were collected from 153 unrelated individuals of Chinese Han population in Shaanxi province. Allele and genotype frequencies for the 6 STR loci were estimated and statistical parameters of polymorphism were calculated. Results 8 alleles and 18 genotypes, 10 alleles and 17 genotypes, 9 alleles and 15 genotypes, 12alleles and 29 genotypes, 12 alleles and 31 genotypes, 8 alleles and 11 genotypes were observed at D12S358, D12S1675, D12S1663, D12S1697, D12S1725 and D12S1613, respectively. No deviations of the observed allele frequency from Hardy-weinberg equilibrium expectations were found for any of these loci. The Heterozygotes of these 6 loci were 78.89%, 66.10%, 54.95%, 79.10%, 71.98% and 59.48%, respectively. It indicated the high genetic polymorphism of the loci in Chinese Han population. Conclusion The 6 STR loci belonged to the genetic marker system of high discriminutesation and high information in Chinese Han population and can be used in the study of gene-related diseases.

  20. Comparative Studies of Population Synthesis Models in the Framework of Modified Strömgren Filters

    Indian Academy of Sciences (India)

    Yuvraj Harsha Sreedhar; Karl Rakos; Gerhard Hensler

    2014-03-01

    Evolutionary models form a vital part of stellar population research in understanding their evolution, but despite their long history of development, they are often misrepresented and the properties of stellar population observed through broadband and spectroscopic measurements are also misinterpreted. With growing numbers of these synthesis models, model comparison becomes an important analysis to choose a suitable model for understanding stellar populations and model up-gradation. Along with model comparison, we reinvestigate the technique ofmodified Strömgren photometry to measure reliable parameter-sensitive colours and estimate precise model ages and metallicities. The assessment of Rakos/Schulz models with GALEV and Worthey’s Lick/IDS model find smaller colour variation: ( - ) ≤ 0.056, ( - ) ≤ -0.05 and ( − ) ≤ 0.061. The study conveys a good agreement of GALEV models with modified Strömgren colours but with poor UV model predictions and observed globular cluster data, while the spectroscopic models perform badly because of outdated isochrone and stellar spectral libraries with inaccurate/insufficient knowledge of various stellar phases and their treatment. Overall, the assessment finds modified Strömgren photometry well suited to study different types stellar populations by mitigating the effects of age-metallicity degeneracy.

  1. Analysis of seventeen Y-chromosome STR loci in the Cape Muslim population of South Africa.

    Science.gov (United States)

    Cloete, Kevin; Ehrenreich, Liezle; D'Amato, María Eugenia; Leat, Neil; Davison, Sean; Benjeddou, Mongi

    2010-01-01

    Two Y-STR genotyping systems were evaluated for usefulness in forensic casework in the Cape Muslim population of South Africa. Samples were collected from 105 males, and genotyped for 17 loci amplified in two multiplexes. Allele and haplotype frequencies were determined for nine Y-STR loci used to define the minimal haplotype (DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, and the duplicated locus DYS385) amplified in one multiplex, as well as for eight widely used loci amplified in a second multiplex and consisting of DYS449, DYS481, DYS518, DYS557, DYS570, DYS607, DYS612 and DYS614. When analysing the samples for all the loci, 104 unique haplotypes were obtained, and the discrimination capacity was 0.990. When considering only the nine Y-STRs included in the minimal haplotype, 91 unique haplotypes were obtained, and the discrimination capacity was 0.866. In the case of the remaining eight Y-STR loci, values of 97 and 0.924 were obtained, respectively. PMID:19962930

  2. Y-STR genetic diversity in autochthonous Andalusians from Huelva and Granada provinces (Spain).

    Science.gov (United States)

    Ambrosio, Beatriz; Novelletto, Andrea; Hernandez, Candela; Dugoujon, Jean Michel; Fortes-Lima, César; Rodriguez, Juan Nicolás; Calderon, Rosario

    2012-03-01

    Seventeen Y-chromosomal short tandem repeats (STRs) were analyzed in 347 healthy, unrelated, autochthonous males from the Andalusian provinces of Huelva (N=167) and Granada (N=180). AmpFlSTR Y-filer PCR Amplification kit (Applied Biosystems) was used to type the Y-STR markers. A total of 156 and 166 different haplotypes for the 17 Y-STR set were detected in Huelva, and Granada, respectively. The same haplotype diversity was found for both samples (0.998±0.001), and the overall discrimination capacity was 0.904. The most common minimal haplotype (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393) in both subpopulations was 14-13-16-24-11-13-13, which is also the most frequent haplotype among Atlantic European populations. Comparison analysis using pairwise R(ST) values and Analysis of Molecular Variance (AMOVA) revealed a significant genetic distance between our Andalusian samples and other ones from the northern Iberian fringe (including Basque and Pyrenean populations). However, results from the multi-dimensional scaling analysis (MDS) yielded a well-defined group of Iberian populations separated from the other Mediterranean clusters observed. PMID:21664894

  3. A population genetic database of cat breeds developed in coordination with a domestic cat STR multiplex.

    Science.gov (United States)

    Menotti-Raymond, Marilyn; David, Victor A; Weir, Bruce S; O'Brien, Stephen J

    2012-05-01

    A simple tandem repeat (STR) PCR-based typing system developed for the genetic individualization of domestic cat samples has been used to generate a population genetic database of domestic cat breeds. A panel of 10 tetranucleotide STR loci and a gender-identifying sequence tagged site (STS) were co-amplified in genomic DNA of 1043 individuals representing 38 cat breeds. The STR panel exhibits relatively high heterozygosity in cat breeds, with an average 10-locus heterozygosity of 0.71, which represents an average of 38 breed-specific heterozygosities for the 10-member panel. When the entire set of breed individuals was analyzed as a single population, a heterozygosity of 0.87 was observed. Heterozygosities obtained for the 10 loci range from 0.72 to 0.96. The power for genetic individualization of domestic cat samples of the multiplex is high, with a probability of match (p(m)) of 6.2E-14, using a conservative θ = 0.05.

  4. Evaluation of the MeltPro TB/STR assay for rapid detection of streptomycin resistance in Mycobacterium tuberculosis.

    Science.gov (United States)

    Zhang, Ting; Hu, Siyu; Li, Guoli; Li, Hui; Liu, Xiaoli; Niu, Jianjun; Wang, Feng; Wen, Huixin; Xu, Ye; Li, Qingge

    2015-03-01

    Rapid and comprehensive detection of drug-resistance is essential for the control of tuberculosis, which has facilitated the development of molecular assays for the detection of drug-resistant mutations in Mycobacterium tuberculosis. We hereby assessed the analytical and clinical performance of an assay for streptomycin-resistant mutations. MeltPro TB/STR is a closed-tube, dual-color, melting curve analysis-based, real-time PCR test designed to detect 15 streptomycin-resistant mutations in rpsL 43, rpsL 88, rrs 513, rrs 514, rrs 517, and rrs 905-908 of M. tuberculosis. Analytical studies showed that the accuracy was 100%, the limit of detection was 50-500 bacilli per reaction, the reproducibility in the form of Tm variation was within 1.0 °C, and we could detect 20% STR resistance in mixed bacterial samples. The cross-platform study demonstrated that the assay could be performed on six models of real-time PCR instruments. A multicenter clinical study was conducted using 1056 clinical isolates, which were collected from three geographically different healthcare units, including 709 STR-susceptible and 347 STR-resistant isolates characterized on Löwenstein-Jensen solid medium by traditional drug susceptibility testing. The results showed that the clinical sensitivity and specificity of the MeltPro TB/STR was 88.8% and 95.8%, respectively. Sequencing analysis confirmed the accuracy of the mutation types. Among all the 8 mutation types detected, rpsL K43R (AAG → AGG), rpsL K88R (AAG → AGG) and rrs 514 A → C accounted for more than 90%. We concluded that MeltPro TB/STR represents a rapid and reliable assay for the detection of STR resistance in clinical isolates.

  5. Developing STR databases on structured populations: the native South Siberian population versus the Russian population.

    Science.gov (United States)

    Zhivotovsky, Lev A; Malyarchuk, Boris A; Derenko, Miroslava V; Wozniak, Marcin; Grzybowski, Tomasz

    2009-09-01

    Developing a forensic DNA database on a population that consists of local ethnic groups separated by physical and cultural barriers is questionable as it can be genetically subdivided. On the other side, small sizes of ethnic groups, especially in alpine regions where they are sub-structured further into small villages, prevent collecting a large sample from each ethnic group. For such situations, we suggest to obtain both a total population database on allele frequencies across ethnic groups and a list of theta-values between the groups and the total data. We have genotyped 558 individuals from the native population of South Siberia, consisting of nine ethnic groups, at 17 autosomal STR loci of the kit packages AmpFlSTR SGM Plus i, Cyrillic AmpFlSTR Profiler Plus. The groups differentiate from each other with average theta-values of around 1.1%, and some reach up to three to four percent at certain loci. There exists between-village differentiation as well. Therefore, a database for the population of South Siberia is composed of data on allele frequencies in the pool of ethnic groups and data on theta-values that indicate variation in allele frequencies across the groups. Comparison to additional data on northeastern Asia (the Chukchi and Koryak) shows that differentiation in allele frequencies among small groups that are separated by large geographic distance can be even greater. In contrast, populations of Russians that live in large cities of the European part of Russia are homogeneous in allele frequencies, despite large geographic distance between them, and thus can be described by a database on allele frequencies alone, without any specific information on theta-values.

  6. STR: a student developed star tracker for the ESA-LED ESMO moon mission

    OpenAIRE

    Lizy-Destrez, Stéphanie; Mimoun, David

    2010-01-01

    In the frame of their engineering degree, ISAE’s students are developing a Star Tracker, with the aim of being the core attitude estimation equipment of the European Moon Student Orbiter. This development goes on since several years and is currently in phase B. We intend to start building an integrated breadboard for the end of the academic year. The STR is composed of several sub-systems: the optical and detection sub-system, the electronics, the mechanics and the software. The optical de...

  7. Strømmålinger ved Sæby Udløbsledning

    DEFF Research Database (Denmark)

    Larsen, Torben

    Med henblik på at vurdere tidevandsstrømningens amplitude i det kystnære område ud for Sæby har man i ca. 14 dage fra 25.5.81 til 11.6.81 haft udlagt en Aanderraa RCM 4 strømmåler ca. 1 m under overfladen på ca. 4 m vanddybde. Måleren la ca. 900 m fra land i en linje, som var en forlængelse af ud...

  8. Y-STR多态性与人类学%The Polymorphism of Y-STR and Anthropology

    Institute of Scientific and Technical Information of China (English)

    刘秀梅; 吴江平; 席焕久

    2007-01-01

    人类Y染色体短串联重复序列(Y-STR)具有父系遗传、缺乏重组、分型简单、信息量大、多态性高等特点,是研究人类的起源进化、民族差异、种族差异、群体及地区分布等的有力工具.本文就Y-STR多态性与其在人类学方面的应用作一综述.

  9. Native American population data based on the Globalfiler(®) autosomal STR loci.

    Science.gov (United States)

    Ng, Jillian; Oldt, Robert F; McCulloh, Kelly L; Weise, Jessica A; Viray, Joy; Budowle, Bruce; Smith, David Glenn; Kanthaswamy, Sreetharan

    2016-09-01

    Native American population data are limited and thus impact computing accurate statistical parameters for forensic investigations. Thus, additional information should be generated from geographically representative tribes in North America, particularly from those that are not included in existing population databases for forensic use. The Globafiler(®) PCR Amplification kit was used to produce STR genotypic data for 533 individuals who represent 31 Native American tribal populations derived from eight geographically diverse regions in North America. Population genetic estimates from 21 autosomal STRs are reported.

  10. Genetic data for the 13 CODIS STR loci in Singapore Chinese.

    Science.gov (United States)

    Syn, C K C; Chuah, S Y; Ang, H C; Lim, S E S; Tan-Siew, W F; Chow, S T; Budowle, Bruce

    2005-09-10

    Allele frequencies for the 13 CODIS STR loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 209 unrelated Chinese in Singapore. The combined random match probability for the 13 loci is about 6.6 x10(-15) and the overall probability of excluding paternity is 0.9999899. The results demonstrate that the loci are useful for forensic human identification and parentage testing for the Chinese population in Singapore.

  11. A case of false mother included with 46 autosomal STR markers

    OpenAIRE

    Li, Li; Lin, Yuan; Liu, Yan; Zhu, Ruxin; Zhao, Zhenmin; Que, Tingzhi

    2015-01-01

    Background For solving a maternity case, 19 autosomal short tandem repeats (STRs) were amplified using the AmpFℓSTR® SinofilerTM kit and PowerPlex® 16 System. Additional 27 autosomal STR loci were analyzed using two domestic kits AGCU 21+1 and STRtyper-10G. The combined maternity index (CMI) was calculated to be 3.3 × 1013, but the putative mother denied that she had given birth to the child. In order to reach an accurate conclusion, further testing of 20 X-chromosomal short tandem repeats (X...

  12. Comparison of allele frequencies of eight STR loci from Argentinian Amerindian and European populations.

    Science.gov (United States)

    Sala, A; Penacino, G; Corach, D

    1998-10-01

    Eight STR systems (THO1, FABP, VWA, FES/FPS, HPRTB, F13A1, CSF1PO, and D6S366) were investigated in different ethnic groups of Argentina. Allele and genotype frequencies, power of exclusion, and discriminative power were investigated. Hardy-Weinberg expectations were calculated from heterozygosity levels. FST and G tests demonstrated that significant differences exist among the investigated populations for some of the eight STRs markers. The Wichi Indians are clearly separated from the Mapuche and Tehuelche, who in turn are closer to the European population, suggesting non-Amerindian admixture.

  13. Y-STR diversity and ethnic admixture in White and Mulatto Brazilian population samples

    OpenAIRE

    Luzitano Brandão Ferreira; Celso Teixeira Mendes-Junior; Cláudia Emília Vieira Wiezel; Marcelo Rizzatti Luizon; Aguinaldo Luiz Simões

    2006-01-01

    We investigated 50 Mulatto and 120 White Brazilians for the Y-chromosome short tandem repeat (Y-STR) markers (DYS19, DYS390, DYS391, DYS392 and DYS393) and found 79 different haplotypes in the White and 35 in the Mulatto sample. Admixture estimates based on allele frequencies showed that the admixture of the white sample was 89% European, 6% African and 5% Amerindian while the Mulatto sample was 93% European and 7% African. Results were consistent with historical records of the directional ma...

  14. Y-STR diversity and ethnic admixture in White and Mulatto Brazilian population samples

    Directory of Open Access Journals (Sweden)

    Luzitano Brandão Ferreira

    2006-01-01

    Full Text Available We investigated 50 Mulatto and 120 White Brazilians for the Y-chromosome short tandem repeat (Y-STR markers (DYS19, DYS390, DYS391, DYS392 and DYS393 and found 79 different haplotypes in the White and 35 in the Mulatto sample. Admixture estimates based on allele frequencies showed that the admixture of the white sample was 89% European, 6% African and 5% Amerindian while the Mulatto sample was 93% European and 7% African. Results were consistent with historical records of the directional mating between European males and Amerindian or African females.

  15. Genetic population study of 11 Y chromosome STR loci in Greece.

    Science.gov (United States)

    Katsaloulis, Panayotis; Tsekoura, Konstantina; Vouropoulou, Maria; Miniati, Penelope

    2013-05-01

    Statistical properties of eleven Y chromosome Short Tandem Repeat (STR) markers were analyzed (DYS391, DYS389I, DYS439, DYS389II, DYS438, DYS437, DYS19, DYS392, DYS393, DYS390 and DYS385) in a Greek population sample. The 200 subjects where distributed across Greece, from various Peripheries. 182 distinct haplotypes were found. To validate our results gene diversity has been calculated for the whole population, as well as for each locus individually. Genetic distance has been estimated between this population and Albanian, Egyptian, Italian and Turkish populations. The results indicate that all Y loci are useful for forensic sciences. PMID:23582698

  16. CFD-Simulation zur Untersuchung des Strömungsverhaltens in Strukturrohren

    OpenAIRE

    Hellwig, Udo; Nikolaus, Hartwig

    2004-01-01

    Die CFD (Computational Fluid Dynamics)-Simulation hat mittlerweile in alle technischen Bereiche Einzug gehalten, in denen die Vorhersage strömungstechnischer Ereignisse relevant ist. Nach wie vor ist allerdings eine Validierung von Rechenergebnissen mit Messdaten unerlässlich, um zu vertrauenswürdigen Simulationsergebnissen zu kommen. Dies ist u. a. darauf zurückzuführen, dass die am Markt verfügbare CFD-Software darauf ausgerichtet ist, ein möglichst breites Anwendungsspektrum zu bedienen. D...

  17. Kystmodellen NorKyst-800 – en strømmodell for hele norskekysten

    OpenAIRE

    Asplin, Lars; Sandvik, Anne D.; Albretsen, Jon

    2011-01-01

    Kystmodellen NorKyst-800 er en beregningsmodell som simulerer bl.a. strøm, saltholdighet og temperatur med 800 meters romlig oppløsning og med høy oppløsning i tid for hele norskekysten. NorKyst-800 er utviklet på Havforskningsinstituttet i samarbeid med Meteorologisk institutt og Niva. :: The coastal model NorKyst-800 is a computer model that simulates variables such as currents, salinity and temperature along the whole Norwegian coast, at an 800 metre spatia...

  18. Introduction of the Python script STRinNGS for analysis of STR regions in FASTQ or BAM files and expansion of the Danish STR sequence database to 11 STRs.

    Science.gov (United States)

    Friis, Susanne L; Buchard, Anders; Rockenbauer, Eszter; Børsting, Claus; Morling, Niels

    2016-03-01

    This work introduces the in-house developed Python application STRinNGS for analysis of STR sequence elements in BAM or FASTQ files. STRinNGS identifies sequence reads with STR loci by their flanking sequences, it analyses the STR sequence and the flanking regions, and generates a report with the assigned SNP-STR alleles. The main output file from STRinNGS contains all sequences with read counts above 1% of the total number of reads per locus. STR sequences are automatically named according to the nomenclature used previously and according to the repeat unit definitions in STRBase (http://www.cstl.nist.gov/strbase/). The sequences are named with (1) the locus name, (2) the length of the repeat region divided by the length of the repeat unit, (3) the sequence(s) of the repeat unit(s) followed by the number of repeats and (4) variations in the flanking regions. Lower case letters in the main output file are used to flag sequences with previously unknown variations in the STRs. SNPs in the flanking regions are named by their "rs" numbers and the nucleotides in the SNP position. Data from 207 Danes sequenced with the Ion Torrent™ HID STR 10-plex that amplified nine STRs (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D16S539, TH01, TPOX, vWA), and Amelogenin was analysed with STRinNGS. Sequencing uncovered five common SNPs near four STRs and revealed 20 new alleles in the 207 Danes. Three short homopolymers in the D8S1179 flanking regions caused frequent sequencing errors. In 29 of 3726 allele calls (0.8%), sequences with homopolymer errors were falsely assigned as true alleles. An in-house developed script in R compensated for these errors by compiling sequence reads that had identical STR sequences and identical nucleotides in the five common SNPs. In the output file from the R script, all SNP-STR haplotype calls were correct. The 207 samples and six additional samples were sequenced for D3S1358, D12S391, and D21S11 using the 454 GS Junior platform in this and a

  19. Topical problems connected with the German act on electricity from renewable energy sources (StrEG); Aktuelle Probleme des Stromeinspeisungsgesetzes

    Energy Technology Data Exchange (ETDEWEB)

    Pohlmann, M. [VDEW, Frankfurt am Main (Germany). Rechtsabteilung

    1998-11-16

    The German act (StrEG) intended to enhance the use of renewable energy sources for electricity generation and to promote the relevant technologies raises some problems in connection with constitutional law that still await judicial review by the German Federal Constitutional Court. In addition, doubts as to the lawfulness of provisions of the act have been emerging in connection with EC laws governing the regime of subsidies and state aid. The article here summarizes the current situation. (orig./CB) [Deutsch] Die verfassungsrechtlichen Fragen des Stromeinspeisungsgesetzes (StrEG) harren noch immer einer Loesung durch das Bundesverfassungsgericht. Auch auf einer anderen Ebene wird die Rechtmaessigkeit des StrEG immer mehr in Zweifel gezogen. Die Probleme der Vereinbarkeit mit dem europaeischen Beihilferecht treten immer offener zutage. Der Beitrag fasst die aktuelle Situation zusammen. (orig./CB)

  20. Capillary electrophoresis and 5-channel LIF detection of a 26plex autosomal STR assay for human identification.

    Science.gov (United States)

    Hill, Carolyn R

    2012-01-01

    Multiplex polymerase chain reaction (PCR) is a common method used for DNA typing in forensic and paternity cases. There are numerous commercial short tandem repeat (STR) multiplex assays currently available to the forensic community. These assays amplify the core Combined DNA Index System (CODIS) STR loci for entry into the US. DNA database. Additional non-CODIS loci, which are considered genetically unlinked to the CODIS loci, can be useful in resolving challenging cases such as missing persons and mass disaster victim identification, paternity testing, and immigration testing. An STR multiplex has been successfully developed with 25 non-CODIS autosomal loci plus the sex-typing locus amelogenin for a total of 26 loci in a single 26plex amplification reaction. This chapter will focus on the preparation and the use of the 26plex assay with DNA samples for the purpose of human identification.

  1. Y-STR analysis on DNA mixture samples--results of a collaborative project of the ENFSI DNA Working Group

    DEFF Research Database (Denmark)

    Parson, Walther; Niederstätter, Harald; Lindinger, Alexandra;

    2008-01-01

    The ENFSI (European Network of Forensic Science Institutes) DNA Working Group undertook a collaborative project on Y-STR typing of DNA mixture samples that were centrally prepared and thoroughly tested prior to the shipment. Four commercial Y-STR typing kits (Y-Filer, Applied Biosystems, Foster...... City, CA, USA; Argus Y Nonaplex, Biotype, Dresden, Germany; Powerplex Y, Promega, Madison, WI, USA; and DYSplex-3, SERAC, Bad Homburg, Germany) were used for the amplification of the mixture samples. The results of the study showed a striking inter-laboratory difference of kit performance as determined...... from the peak heights of the obtained Y-STR genotypes. Variation in quantity and quality of the shipped DNA can be excluded as reason for the observed differences because both samples and shipping conditions were found to be reproducible in an earlier study. The results suggest that in some cases...

  2. Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172.

    Science.gov (United States)

    Boll, M; Fuchs, G

    1995-12-15

    Anoxic metabolism of many aromatic compounds proceeds via the common intermediate benzoyl-CoA. Benzoyl-CoA is dearomatized by benzoyl-CoA reductase (dearomatizing) in a two-electron reduction step, possibly yielding cyclohex-1,5-diene-1-carboxyl-CoA. This process has to overcome a high activation energy and is considered a biological Birch reduction. The central, aromatic-ring-reducing enzyme was investigated for the first time in the denitrifying bacterium Thauera aromatica strain K172. A spectrophotometric assay was developed which was strictly dependent on MgATP, both with cell extract and with purified enzyme. The oxygen-sensitive new enzyme was purified 35-fold with 20% yield under anaerobic conditions in the presence of 0.25 mM dithionite. It had a native molecular mass of approximately 170 kDa and consisted of four subunits a,b,c,d of 48, 45, 38 and 32 kDa. The oligomer composition of the protein most likely is abcd. The ultraviolet/visible spectrum of the protein as isolated, but without dithionite, was characteristic for an iron-sulfur protein with an absorption maximum at 279 nm and a broad shoulder at 390 nm. The estimated molar absorption coefficient at 390 nm was 35,000 M-1 cm-1. Reduction of the enzyme by dithionite resulted in a decrease of absorbance at 390 nm, and the colour turned from greenish-brown to red-brown. The enzyme contained 10.8 +/- 1.5 mol Fe and 10.5 +/- 1.5 mol acid-labile sulfur/mol. Besides zinc (0.5 mol/mol protein) no other metals nor selenium could be detected in significant amounts. The enzyme preparation contained a flavin or flavin-like compound; the estimated content was 0.3 mol/mol enzyme. The enzyme reaction required MgATP and a strong reductant such as Ti(III). The reaction catalyzed is: benzoyl-CoA + 2 Ti(III) + n ATP-->non-aromatic acyl-CoA + 2 Ti(IV) + n ADP + n Pi. The estimated number n of ATP molecules hydrolyzed/two electrons transferred in benzoyl-CoA reduction is 2-4. In the absence of benzoyl-CoA the enzyme

  3. Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases.

    Science.gov (United States)

    Purps, Josephine; Geppert, Maria; Nagy, Marion; Roewer, Lutz

    2015-11-01

    DNA testing is an established part of the investigation and prosecution of sexual assault. The primary purpose of DNA evidence is to identify a suspect and/or to demonstrate sexual contact. However, due to highly uneven proportions of female and male DNA in typical stains, routine autosomal analysis often fails to detect the DNA of the assailant. To evaluate the forensic efficiency of the combined application of autosomal and Y-chromosomal short tandem repeat (STR) markers, we present a large retrospective casework study of probative evidence collected in sexual-assault cases. We investigated up to 39 STR markers by testing combinations of the 16-locus NGMSElect kit with both the 23-locus PowerPlex Y23 and the 17-locus Yfiler kit. Using this dual approach we analyzed DNA extracts from 2077 biological stains collected in 287 cases over 30 months. To assess the outcome of the combined approach in comparison to stand-alone autosomal analysis we evaluated informative DNA profiles. Our investigation revealed that Y-STR analysis added up to 21% additional, highly informative (complete, single-source) profiles to the set of reportable autosomal STR profiles for typical stains collected in sexual-assault cases. Detection of multiple male contributors was approximately three times more likely with Y-chromosomal profiling than with autosomal STR profiling. In summary, 1/10 cases would have remained inconclusive (and could have been dismissed) if Y-STR analysis had been omitted from DNA profiling in sexual-assault cases. PMID:26280567

  4. D5S2500 is an ambiguously characterized STR: Identification and description of forensic microsatellites in the genomics age.

    Science.gov (United States)

    Phillips, C; Parson, W; Amigo, J; King, J L; Coble, M D; Steffen, C R; Vallone, P M; Gettings, K B; Butler, J M; Budowle, B

    2016-07-01

    In the process of establishing short tandem repeat (STR) sequence variant nomenclature guidelines in anticipation of expanded forensic multiplexes for massively parallel sequencing (MPS), it was discovered that the STR D5S2500 has multiple positions and genomic characteristics reported. This ambiguity is because the marker named D5S2500 consists of two different microsatellites forming separate components in the capillary electrophoresis multiplexes of Qiagen's HDplex (Hilden, Germany) and AGCU ScienTech's non-CODIS STR 21plex (Wuxi, Jiangsu, China). This study outlines the genomic details used to identify each microsatellite and reveals the D5S2500 marker in HDplex has the correctly assigned STR name, while the D5S2500 marker in the AGCU 21plex, closely positioned a further 1643 nucleotides in the human reference sequence, is an unnamed microsatellite. The fact that the D5S2500 marker has existed as two distinct STR loci undetected for almost ten years, even with reported discordant genotypes for the standard control DNA, underlines the need for careful scrutiny of the genomic properties of forensic STRs, as they become adapted for sequence analysis with MPS systems. We make the recommendation that precise chromosome location data must be reported for any forensic marker under development but not in common use, so that the genomic characteristics of the locus are validated to the same level of accuracy as its allelic variation and forensic performance. To clearly differentiate each microsatellite, we propose the name D5S2800 be used to identify the Chromosome-5 STR in the AGCU 21plex.

  5. Genetic analysis of 15 autosomal and 12 Y-STR loci in the Espirito Santo State population, Brazil.

    Science.gov (United States)

    Wolfgramm, Eldamária de Vargas; Silva, Beatriz Candida; Aguiar, Vitor Resende da Costa; Malta, Frederico Scott Varela; de Castro, Amanda Mafia; Ferreira, Alessandro Clayton de Souza; Prezoti, Alessandra Nunes Loureiro; de Paula, Flavia; Louro, Iúri Drumond

    2011-06-01

    This study provides population genetic data for individuals of Vitoria, Espirito Santo, Brazil, a location not yet characterized for STR frequencies used for genetic identification studies. Allelic frequencies and other population data analysis are reported for the 15 autosomal-STR loci included in the PowerPlex(®)16 kit (CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, Penta D, Penta E, TPOX, TH01 and vWA). Allele and haplotype frequencies, gene diversity and discrimination capacity were also estimated for the PowerPlex(®) Y System (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439). Blood samples were obtained from 226 unrelated volunteers (135 males and 91 females) residents in the city of Vitoria, representing a typical sample of the mixed ethnicity present in the Espirito Santo State, Brazil. Within the tested population, the total number of individuals typed for specific markers is: 226 for D13S317, D21S11, D3S1358, D7S820, D8S1179 and FGA; 225 for D16S539 and D5S818; 224 for D18S51; 223 for CSF1PO; 222 for Penta D and vWA; 220 for Penta E; 207 for TPOX and 142 for TH01. Y-STR haplotypes were analyzed for 102 unrelated males, being 71 of them present in the 135 autosomal-STR sample, and 31 new males tested only for Y-STR markers. All autosomal markers were in Hardy-Weinberg Equilibrium. Y-STR analysis identified 101 haplotypes, being 100 of them unique. PMID:20965142

  6. Y-STR variation in the Basque diaspora in the Western USA: evolutionary and forensic perspectives.

    Science.gov (United States)

    Valverde, Laura; Rosique, Melania; Köhnemann, Stephan; Cardoso, Sergio; García, Ainara; Odriozola, Adrián; Aznar, Jose María; Celorrio, David; Schuerenkamp, Marianne; Zubizarreta, Josu; Davis, Michael C; Hampikian, Greg; Pfeiffer, Heidi; de Pancorbo, Marian M

    2012-03-01

    Individuals of Basque origin migrated in large numbers to the Western USA in the second half of the nineteenth century, and the flow continued with less intensity during the last century. The European source population, that of the Basque Country, has long been a cultural and geographical isolate. Previous studies have demonstrated that Y-STR frequencies of Basques are different from those of other Spanish and European populations [1]. The Basque diaspora in the Western USA is a recent migration, but the founder effect and the incorporation of new American Y chromosomes into the paternal genetic pool of the Basque diaspora could have influenced its genetic structure and could thus have practical implications for forensic genetics. To check for genetic substructure among the European source and Basque diaspora populations and determine the most suitable population database for the Basque diaspora in the Western USA, we have analysed the haplotype distribution of 17 Y-STRs in both populations. We have found that the Basque diaspora in the Western USA largely conserve the Y chromosome lineage characteristic of the autochthonous European Basque population with no statistically significant differences. This implies that a common 17 Y-STR Basque population database could be used to calculate identification or kinship parameters regardless of whether the Basque individuals are from the European Basque Country or from the Basque diaspora in the Western USA. PMID:22081043

  7. Analysis of 17 STR data on 5362 southern Portuguese individuals-an update on reference database.

    Science.gov (United States)

    Cabezas Silva, Raquel; Ribeiro, Teresa; Lucas, Isabel; Porto, Maria João; Costa Santos, Jorge; Dario, Paulo

    2016-03-01

    The main objective of this work consisted of the updating of allele frequencies and other relevant forensic parameters for the 17 autosomal STR loci provided by the combination of the two types of kits used routinely in our laboratory casework: AmpF/STR Identifiler(®) and the Powerplex(®) 16 Systems. This aim was of significant importance, given that the last study on these kits within the southern Portuguese population dates back to 2006, and, as a consequence, it was necessary to correct the deviation caused by population evolution over the last ten years so that they might be better applied to our forensic casework. For this reason genetic data from 5362 unrelated Caucasian Portuguese individuals from the south of Portugal who were involved in paternity testing casework from 2005 to 2014 was used. Of all the markers, TPOX proved to be the least polymorphic, and Penta E the most. Secondly, this up-to-date southern Portuguese population was compared not only with the northern and central Portuguese populations, but also with that of southern Portugal in 2006, along with populations from Spain, Italy, Greece, Romania, Morocco, Angola and Korea in order to infer information about the relatedness of these respective populations, and the variation of the southern Portuguese population over time.

  8. Systematic evaluation of sensitivity and specificity of sibship determination by using 15 STR loci.

    Science.gov (United States)

    Pu, Chang En; Linacre, Adrian

    2008-07-01

    Paternity disputes and other forms of kinship testing are routinely resolved using short tandem repeat (STR) DNA loci. Sibship determination is encountered in instances where the DNA profiles of two individuals are compared to determine if they are siblings. If either parent is available for testing then the situation is simplified but if neither parent of the two individuals is available for DNA testing, a combined sibling indices (CSI) for the determination of sibship between two people can be determined. Support for kinship is also based upon the sharing of alleles, particularly when both alleles are shared at the same locus, termed two-allele-sharing-loci (TASL). We report on the combination of CSI and TASL to enhance the determination of sibship. The 15 STR loci that comprise the Identifiler loci were applied to three populations using pairs of full siblings or unrelated pairs. Based upon the data obtained, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) can be applied to determining whether two DNA profiles come from full or non-sibling pairs. This report highlights the problems inherent in this form of kinship testing and recommends a combination use of CSI and TASL for sibship determination.

  9. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci

    Science.gov (United States)

    Purps, Josephine; Siegert, Sabine; Willuweit, Sascha; Nagy, Marion; Alves, Cíntia; Salazar, Renato; Angustia, Sheila M.T.; Santos, Lorna H.; Anslinger, Katja; Bayer, Birgit; Ayub, Qasim; Wei, Wei; Xue, Yali; Tyler-Smith, Chris; Bafalluy, Miriam Baeta; Martínez-Jarreta, Begoña; Egyed, Balazs; Balitzki, Beate; Tschumi, Sibylle; Ballard, David; Court, Denise Syndercombe; Barrantes, Xinia; Bäßler, Gerhard; Wiest, Tina; Berger, Burkhard; Niederstätter, Harald; Parson, Walther; Davis, Carey; Budowle, Bruce; Burri, Helen; Borer, Urs; Koller, Christoph; Carvalho, Elizeu F.; Domingues, Patricia M.; Chamoun, Wafaa Takash; Coble, Michael D.; Hill, Carolyn R.; Corach, Daniel; Caputo, Mariela; D’Amato, Maria E.; Davison, Sean; Decorte, Ronny; Larmuseau, Maarten H.D.; Ottoni, Claudio; Rickards, Olga; Lu, Di; Jiang, Chengtao; Dobosz, Tadeusz; Jonkisz, Anna; Frank, William E.; Furac, Ivana; Gehrig, Christian; Castella, Vincent; Grskovic, Branka; Haas, Cordula; Wobst, Jana; Hadzic, Gavrilo; Drobnic, Katja; Honda, Katsuya; Hou, Yiping; Zhou, Di; Li, Yan; Hu, Shengping; Chen, Shenglan; Immel, Uta-Dorothee; Lessig, Rüdiger; Jakovski, Zlatko; Ilievska, Tanja; Klann, Anja E.; García, Cristina Cano; de Knijff, Peter; Kraaijenbrink, Thirsa; Kondili, Aikaterini; Miniati, Penelope; Vouropoulou, Maria; Kovacevic, Lejla; Marjanovic, Damir; Lindner, Iris; Mansour, Issam; Al-Azem, Mouayyad; Andari, Ansar El; Marino, Miguel; Furfuro, Sandra; Locarno, Laura; Martín, Pablo; Luque, Gracia M.; Alonso, Antonio; Miranda, Luís Souto; Moreira, Helena; Mizuno, Natsuko; Iwashima, Yasuki; Neto, Rodrigo S. Moura; Nogueira, Tatiana L.S.; Silva, Rosane; Nastainczyk-Wulf, Marina; Edelmann, Jeanett; Kohl, Michael; Nie, Shengjie; Wang, Xianping; Cheng, Baowen; Núñez, Carolina; Pancorbo, Marian Martínez de; Olofsson, Jill K.; Morling, Niels; Onofri, Valerio; Tagliabracci, Adriano; Pamjav, Horolma; Volgyi, Antonia; Barany, Gusztav; Pawlowski, Ryszard; Maciejewska, Agnieszka; Pelotti, Susi; Pepinski, Witold; Abreu-Glowacka, Monica; Phillips, Christopher; Cárdenas, Jorge; Rey-Gonzalez, Danel; Salas, Antonio; Brisighelli, Francesca; Capelli, Cristian; Toscanini, Ulises; Piccinini, Andrea; Piglionica, Marilidia; Baldassarra, Stefania L.; Ploski, Rafal; Konarzewska, Magdalena; Jastrzebska, Emila; Robino, Carlo; Sajantila, Antti; Palo, Jukka U.; Guevara, Evelyn; Salvador, Jazelyn; Ungria, Maria Corazon De; Rodriguez, Jae Joseph Russell; Schmidt, Ulrike; Schlauderer, Nicola; Saukko, Pekka; Schneider, Peter M.; Sirker, Miriam; Shin, Kyoung-Jin; Oh, Yu Na; Skitsa, Iulia; Ampati, Alexandra; Smith, Tobi-Gail; Calvit, Lina Solis de; Stenzl, Vlastimil; Capal, Thomas; Tillmar, Andreas; Nilsson, Helena; Turrina, Stefania; De Leo, Domenico; Verzeletti, Andrea; Cortellini, Venusia; Wetton, Jon H.; Gwynne, Gareth M.; Jobling, Mark A.; Whittle, Martin R.; Sumita, Denilce R.; Wolańska-Nowak, Paulina; Yong, Rita Y.Y.; Krawczak, Michael; Nothnagel, Michael; Roewer, Lutz

    2014-01-01

    In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent. PMID:24854874

  10. Genetic polymorphism of 14 non-CODIS STR loci for forensic use in southeast China population.

    Science.gov (United States)

    Shi, Meisen; Yu, Xiaojun; Bai, Rufeng; Shu, Xiji; Zhu, Guanghui; Lv, Junyao; Tu, Youhua

    2008-01-15

    We investigated 14 polymorphic STR loci (D1S2142, D2S1360, D3S1545, D7S1517, D10S2325, D12S391, D13S1492, D14S306, D15S659, D16S3253, D18S1270, D19S253, D20S470, D21S1437) which are not included in the standard sets of forensic loci (CODIS) in a sample of 216 unrelated healthy southeast Chinese individuals. The studied loci were highly informative and did not show departures from Hardy-Weinberg equilibrium. The accumulated powers of discrimination and power of exclusion for the 14 loci were 99.9999999999 and 99.999998%, respectively. No linkage was observed between the 14 loci and the traditional set of STR markers included in commercially available kits (the AmpFLSTR IdentifilerTM 15 System loci). We thus considered the studied 14 STRs are informative and when necessary, can be used as the candidate genetic markers in the study and application in genetics and forensic practice.

  11. Strömgren survey for asteroseismology and galactic archaeology: Let the saga begin

    Energy Technology Data Exchange (ETDEWEB)

    Casagrande, L.; Dotter, A.; Milone, A. P.; Marino, A. F.; Asplund, M.; Schlesinger, K. J. [Research School of Astronomy and Astrophysics, Mount Stromlo Observatory, The Australian National University, Canberra, ACT 2611 (Australia); Aguirre, V. Silva; Lund, M. N.; Grundahl, F.; Nissen, P. E. [Stellar Astrophysics Centre, Department of Physics and Astronomy, Aarhus University, Ny Munkegade 120, DK-8000 Aarhus C (Denmark); Stello, D. [Sydney Institute for Astronomy (SIfA), School of Physics, University of Sydney, Sydney, NSW 2006 (Australia); Huber, D. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Serenelli, A. M. [Institute of Space Sciences (IEEC-CSIC), Campus UAB, Fac. Ciéncies, Torre C5 parell 2, E-08193 Bellaterra (Spain); Cassisi, S.; Pietrinferni, A. [INAF-Osservatorio Astronomico di Collurania, via Maggini, I-64100 Teramo (Italy); Hodgkin, S. [Institute of Astronomy, Madingley Road, Cambridge CB3 0HA (United Kingdom); Feltzing, S. [Lund Observatory, Department of Astronomy and Theoretical Physics, P.O. Box 43, SE-22100 Lund (Sweden); Flynn, C. [Centre for Astrophysics and Supercomputing, Swinburne University of Technology, Hawthorn, VIC 3122 (Australia); Schönrich, R. [Department of Astronomy, The Ohio State University, 140 West 18th Avenue, Columbus, OH 43210-1173 (United States); Wang, W., E-mail: luca.casagrande@anu.edu.au [National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China)

    2014-06-01

    Asteroseismology has the capability of precisely determining stellar properties that would otherwise be inaccessible, such as radii, masses, and thus ages of stars. When coupling this information with classical determinations of stellar parameters, such as metallicities, effective temperatures, and angular diameters, powerful new diagnostics for Galactic studies can be obtained. The ongoing Strömgren survey for Asteroseismology and Galactic Archaeology has the goal of transforming the Kepler field into a new benchmark for Galactic studies, similar to the solar neighborhood. Here we present the first results from a stripe centered at a Galactic longitude of 74° and covering latitude from about 8° to 20°, which includes almost 1000 K giants with seismic information and the benchmark open cluster NGC 6819. We describe the coupling of classical and seismic parameters, the accuracy as well as the caveats of the derived effective temperatures, metallicities, distances, surface gravities, masses, and radii. Confidence in the achieved precision is corroborated by the detection of the first and secondary clumps in a population of field stars with a ratio of 2 to 1 and by the negligible scatter in the seismic distances among NGC 6819 member stars. An assessment of the reliability of stellar parameters in the Kepler Input Catalog is also performed, and the impact of our results for population studies in the Milky Way is discussed, along with the importance of an all-sky Strömgren survey.

  12. Analysis of Y-chromosomal short tandem repeat (STR) polymorphism in an Iranian Sadat population.

    Science.gov (United States)

    Rafiee, M R; Sokhansanj, A; Naghizadeh, M A; Farazmand, A

    2009-08-01

    The molecular genotyping of individuals and reconstruction of kinship through short and highly polymorphic DNA markers, so called short tandem repeats (STR), has become one of the important and efficient methods in anthropology studies and forensic science. Although many populations have been analyzed, no study has yet been carried out on Sadat populations who are putative descendents of Prophet Mohammad (peace be upon him). Polymorphisms of 6 Y-STR loci (DYS19, DYS385a/b, DYS389II, DYS390, DYS392, and DYS393) have been studied in an unrelated population of Sadat males. The aim of this study was to find possible similarities within Sadat males, resided in Iran. Among Sadat, DYS385b was proved to be the most polymorphic (GD = 0.8588), and DYS392 showed the lowest polymorphism (GD = 0.3527). In 50 samples, 45 different haplotypes were found, of which 39 haplotypes were unique. In the study, three samples had multi-allelic patterns. Haplotype diversity, in regard to these 7 markers was 0.9942. PMID:19769300

  13. Population data for 12 Y-chromosome STR loci in a sample from El Salvador.

    Science.gov (United States)

    Monterrosa, Juan Carlos; Morales, Josefina A; Yurrebaso, Iñaki; Gusmão, Leonor; García, Oscar

    2010-01-01

    Haplotype, allele frequencies and population data of 12 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 were determined from a sample of 150 unrelated male individuals from El Salvador, Central America. A total of 131 haplotypes were identified by the 12 Y-STR loci of which 118 were unique. The haplotype diversity (99.08%) and the proportion of different haplotypes (87.33%) were estimated. R(ST) genetic distances were calculated between El Salvador and other populations from Southern and Central America, Europe and Africa. The highest R(ST) genetic distances were found when comparing El Salvador with African populations (0.334

  14. Population data for 12 Y-chromosome STR loci in a sample from Honduras.

    Science.gov (United States)

    Matamoros, Mireya; Yurrebaso, Iñaki; Gusmão, Leonor; García, Oscar

    2009-09-01

    Haplotype, allele frequencies and population data of 12 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 were determined from a sample of 128 unrelated male individuals from Honduras, Central America. A total of 112 haplotypes were identified by the 12 Y-STR loci of which 98 were unique. The haplotype diversity (98.99%) and the proportion of different haplotypes (87.50%) were estimated. Genetic distances were calculated between Honduras and other populations from Southern and Central America, Europe and Africa. The analysis of a Multi Dimensional Scaling (MDS) plot, based on pairwise R(ST) genetic distances, allowed to conclude that Honduras is highly differentiated from the African samples (0.343< or =R(ST)< or =0.620; P=0.000) and from a Native American sample from Argentina, Tobas (R(ST)=0.210, P=0.000). Honduras showed a lower genetic distance to the European cluster (composed by European and South American general population samples from Brazil, Argentina, Colombia and Venezuela) than to the Central American cluster (Mexico and El Salvador). PMID:19628418

  15. Mutation rate at 17 Y-STR loci in "Father/Son" pairs from moroccan population.

    Science.gov (United States)

    Laouina, Adil; Nadifi, Sellama; Boulouiz, Redouane; El Arji, Marzouk; Talbi, Jalal; El Houate, Brahim; Yahia, Hakima; Chbel, Faiza

    2013-09-01

    Precise knowledge of mutation rate at Y-STRs loci is essential for a correct evaluation of typing results in forensic casework and specially kinship genetic studies. In this study, we have examined 252 confirmed and unrelated father/son sample pairs from Moroccan population using the 17 Y-STR markers DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4 of the AmpFlSTR Yfiler™ kit used in routine casework. We observed a total of 15 single repeat mutations between fathers and sons as mutational events. Nine mutations resulted in the gain of a repeat in the son and six resulted in a loss of a repeat. The average mutation rate in the studied sample is 3.5×10(-3) (95% CI 2-5.8×10(-3)). Furthermore, Y-STRs mutation occurrence seems to be 4 times more frequent than autosomal STRs mutation in this sample. PMID:23623014

  16. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci.

    Science.gov (United States)

    Purps, Josephine; Siegert, Sabine; Willuweit, Sascha; Nagy, Marion; Alves, Cíntia; Salazar, Renato; Angustia, Sheila M T; Santos, Lorna H; Anslinger, Katja; Bayer, Birgit; Ayub, Qasim; Wei, Wei; Xue, Yali; Tyler-Smith, Chris; Bafalluy, Miriam Baeta; Martínez-Jarreta, Begoña; Egyed, Balazs; Balitzki, Beate; Tschumi, Sibylle; Ballard, David; Court, Denise Syndercombe; Barrantes, Xinia; Bäßler, Gerhard; Wiest, Tina; Berger, Burkhard; Niederstätter, Harald; Parson, Walther; Davis, Carey; Budowle, Bruce; Burri, Helen; Borer, Urs; Koller, Christoph; Carvalho, Elizeu F; Domingues, Patricia M; Chamoun, Wafaa Takash; Coble, Michael D; Hill, Carolyn R; Corach, Daniel; Caputo, Mariela; D'Amato, Maria E; Davison, Sean; Decorte, Ronny; Larmuseau, Maarten H D; Ottoni, Claudio; Rickards, Olga; Lu, Di; Jiang, Chengtao; Dobosz, Tadeusz; Jonkisz, Anna; Frank, William E; Furac, Ivana; Gehrig, Christian; Castella, Vincent; Grskovic, Branka; Haas, Cordula; Wobst, Jana; Hadzic, Gavrilo; Drobnic, Katja; Honda, Katsuya; Hou, Yiping; Zhou, Di; Li, Yan; Hu, Shengping; Chen, Shenglan; Immel, Uta-Dorothee; Lessig, Rüdiger; Jakovski, Zlatko; Ilievska, Tanja; Klann, Anja E; García, Cristina Cano; de Knijff, Peter; Kraaijenbrink, Thirsa; Kondili, Aikaterini; Miniati, Penelope; Vouropoulou, Maria; Kovacevic, Lejla; Marjanovic, Damir; Lindner, Iris; Mansour, Issam; Al-Azem, Mouayyad; Andari, Ansar El; Marino, Miguel; Furfuro, Sandra; Locarno, Laura; Martín, Pablo; Luque, Gracia M; Alonso, Antonio; Miranda, Luís Souto; Moreira, Helena; Mizuno, Natsuko; Iwashima, Yasuki; Neto, Rodrigo S Moura; Nogueira, Tatiana L S; Silva, Rosane; Nastainczyk-Wulf, Marina; Edelmann, Jeanett; Kohl, Michael; Nie, Shengjie; Wang, Xianping; Cheng, Baowen; Núñez, Carolina; Pancorbo, Marian Martínez de; Olofsson, Jill K; Morling, Niels; Onofri, Valerio; Tagliabracci, Adriano; Pamjav, Horolma; Volgyi, Antonia; Barany, Gusztav; Pawlowski, Ryszard; Maciejewska, Agnieszka; Pelotti, Susi; Pepinski, Witold; Abreu-Glowacka, Monica; Phillips, Christopher; Cárdenas, Jorge; Rey-Gonzalez, Danel; Salas, Antonio; Brisighelli, Francesca; Capelli, Cristian; Toscanini, Ulises; Piccinini, Andrea; Piglionica, Marilidia; Baldassarra, Stefania L; Ploski, Rafal; Konarzewska, Magdalena; Jastrzebska, Emila; Robino, Carlo; Sajantila, Antti; Palo, Jukka U; Guevara, Evelyn; Salvador, Jazelyn; Ungria, Maria Corazon De; Rodriguez, Jae Joseph Russell; Schmidt, Ulrike; Schlauderer, Nicola; Saukko, Pekka; Schneider, Peter M; Sirker, Miriam; Shin, Kyoung-Jin; Oh, Yu Na; Skitsa, Iulia; Ampati, Alexandra; Smith, Tobi-Gail; Calvit, Lina Solis de; Stenzl, Vlastimil; Capal, Thomas; Tillmar, Andreas; Nilsson, Helena; Turrina, Stefania; De Leo, Domenico; Verzeletti, Andrea; Cortellini, Venusia; Wetton, Jon H; Gwynne, Gareth M; Jobling, Mark A; Whittle, Martin R; Sumita, Denilce R; Wolańska-Nowak, Paulina; Yong, Rita Y Y; Krawczak, Michael; Nothnagel, Michael; Roewer, Lutz

    2014-09-01

    In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent. PMID:24854874

  17. Genetic data from Y chromosome STR and SNP loci in Ukrainian population.

    Science.gov (United States)

    Mielnik-Sikorska, Marta; Daca, Patrycja; Woźniak, Marcin; Malyarchuk, Boris A; Bednarek, Jarosław; Dobosz, Tadeusz; Grzybowski, Tomasz

    2013-01-01

    We have tested a sample of 154 unrelated males from Lviv region (Ukraine) for 11 Y-chromosomal single nucleotide polymorphisms (SNPs) and 17 Y-chromosomal STR loci (DYS19, DYS385a, DYS385b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, YGATA_H4.1). Haplotype and haplogroup diversity values were calculated for the population under study. Genetic distances (R(ST)) to 9 other Slavic populations were calculated based on 12 Y-STR loci. Haplotype frequencies and MDS plots were constructed based on genetic distances. Haplogroup frequency patterns revealed in Ukraine are similar to those characteristic of other European populations. However, it also allowed for identification a specific genetic component in Ukrainian sample which seems to originate from areas dwelled by Western Slavs, i.e. subhaplogroup R1a1a7, at frequency of 13.65%. Analysis of R(ST) distances and AMOVA revealed high level of heterogeneity between Slavic populations inhabiting the south and north part of Europe, determined geographically rather than by linguistic factors. It has also been found a closer similarity (in the values of R(ST)) between Ukrainian and Slovak populations than between Ukrainians and other Slavic population samples. PMID:22673612

  18. Population data for 12 Y-chromosome STR loci in a sample from Honduras.

    Science.gov (United States)

    Matamoros, Mireya; Yurrebaso, Iñaki; Gusmão, Leonor; García, Oscar

    2009-09-01

    Haplotype, allele frequencies and population data of 12 Y-chromosome STR loci DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439 were determined from a sample of 128 unrelated male individuals from Honduras, Central America. A total of 112 haplotypes were identified by the 12 Y-STR loci of which 98 were unique. The haplotype diversity (98.99%) and the proportion of different haplotypes (87.50%) were estimated. Genetic distances were calculated between Honduras and other populations from Southern and Central America, Europe and Africa. The analysis of a Multi Dimensional Scaling (MDS) plot, based on pairwise R(ST) genetic distances, allowed to conclude that Honduras is highly differentiated from the African samples (0.343Honduras showed a lower genetic distance to the European cluster (composed by European and South American general population samples from Brazil, Argentina, Colombia and Venezuela) than to the Central American cluster (Mexico and El Salvador).

  19. Gerstmann, Sträussler, and Scheinker: the persecution of the men behind the syndrome.

    Science.gov (United States)

    Zeidman, Lawrence A; Ziller, Matthias Georg; Shevell, Michael

    2014-07-15

    In 1936, Austrian neuroscientists Josef Gerstmann and Ernst Sträussler, along with expatriate Russian neuroscientist Ilya Mark Scheinker, described the familial prion disorder later named for them from a case they mutually treated at a Viennese neurologic hospital. In 1938, Austria was annexed to Nazi Germany in the Anschluss, effectively ending any collaboration between the 3 men. Gerstmann and Scheinker eventually immigrated to America, and Sträussler, although dismissed from his faculty position, remained protected from persecution in Vienna throughout the war likely because of his marriage to an "Aryan woman." Although he attained some degree of success in exile, Gerstmann was never again director of a hospital and primarily maintained a private practice after some brief consulting positions in New York in the 1940s. His medical degree was retroactively stripped by the Nazis without his knowledge, and was not reinstated until 1955. Gerstmann also became embroiled in a bitter struggle to regain his confiscated property in Vienna. Scheinker, aided by the refugee resettlement committee, settled in Cincinnati where he had several successful years and published 3 textbooks, but was denied university tenure and entered private practice until his untimely death. All 3 neurologists lost significant career momentum, and had to pick up the pieces of their fractured lives after the war or their forced exile. Their stories reflect many of the tragic realities of Nazi persecution of Jewish physicians.

  20. Menstrual cycle phase at the time of rape does not affect recovery of semen or amplification of STR profiles of a suspect in vaginal swabs.

    Science.gov (United States)

    Cerdas, Loreley; Herrera, Fabiola; Arrieta, Glenn; Morelli, Concepción; Álvarez, Karla; Gómez, Aarón

    2016-02-01

    The effect of women menstrual cycle on the forensic analysis of rapes was studied in a random group of 170 victims aged among 10 and 51 years. Participants were grouped according to the day of the menstrual cycle in which they were at the moment of the assault. From each participant, samples of vaginal fluid were taken and analyzed for sperm cells, p30 protein, total human DNA and human male DNA. Moreover, amplification of suspect's autosomal STR and Y-STR was attempted. Suspects' autosomal STR profiles were obtained from 92 of the 101 samples in which spermatozoa were found; and Y-STR haplotype was obtained in 1 of the 9 samples where autosomal STR profiles of a male were not obtained. On the other hand, Y-STR haplotypes were obtained in 2 of the 21 samples negative for sperm cells but positive for p30 protein. Y-STR haplotypes were also obtained in 11 of the 48 samples negative for sperm cells and p30 protein. It was found that groups of participants did not differ on the recovery of sperm cells from the vaginal swabs, quantification of suspect's DNA or amplification of their STR profiles. It is concluded that the menstrual cycle phase at the moment of the sexual assault does not affect the main outcomes of the forensic investigation of rapes. PMID:26734988

  1. Polimorfismos de 17 marcadores STR del cromosoma-Y en una muestra poblacional del altiplano cundiboyacense*

    Directory of Open Access Journals (Sweden)

    Korina María Rojas

    2011-04-01

    Full Text Available Introducción: La tipificación molecular de ADN de cromosoma-Y es una herramienta de reconocida importancia en el proceso de identificación de individuos de género masculino en diversos casos forenses. Actualmente es una herramienta de apoyo para los laboratorios de genética estatales en la identificación de víctimas del conflicto armado en Colombia, dentro de los procesos enmarcados en la Ley de Justicia y Paz. En este estudiose determina el haplotipo del cromosoma-Y que será comparado con parientes por línea paaterna de género masculino, con el fin de realizar el análisis estadístico de estos marcadores, aportar a una base de datos colombiana y comparar con parientes de la línea masculina. Objetivo: Realizar una caracterización de haplotipos mediante análisis de marcadores moleculares, STR del cromosoma-Y en una muestra de población del altiplano cundiboyacense colombiano. Metodología: De las muestras del «Programa de Banco de muestras de sangre de funcionarios de la Fiscalía General de la Nación (FGN» fueron seleccionadas aleatoriamente 74 muestras de individuos que no reportaron relación por línea paterna entre sí y cuyo origen geográfico por línea paterna dos generaciones atrás correspondía a los departamentos de Cundinamarca o Boyacá. Se amplificaron y tipificaron 17 polimorfismos de repeticiones en tandem en el cromosoma-Y, el que se aplicó el kit comercial AmpFiSTR®YFiler™ (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438 y DYS448. Resultados: Distribución de frecuencias alélicas y haplotípicas para los 17 marcadores analizados: se determinaron 71 haplotipos con 17 marcadores STR-Y, de los que 68 eran únicos y 3 fueron observados en 2 individuos con un haplotipo compartido para los dos departamentos del altiplano cundiboyacense. La diversidad haplotípica para la muestra poblacional fue de 99.9% con un poder de discriminaci

  2. Polimorfismos de 17 marcadores STR del cromosoma-Y en una muestra poblacional del altiplano cundiboyacense

    Directory of Open Access Journals (Sweden)

    Korina María Rojas

    2011-03-01

    Full Text Available Introducción: La tipificación molecular de ADN de cromosoma-Y es una herramienta de reconocida importancia en el proceso de identificación de individuos de género masculino en diversos casos forenses. Actualmente es una herramienta de apoyo para los laboratorios de genética estatales en la identificación de víctimas del conflicto armado en Colombia, dentro de los procesos enmarcados en la Ley de Justicia y Paz. En este estudiose determina el haplotipo del cromosoma-Y que será comparado con parientes por línea paaterna de género masculino, con el fin de realizar el análisis estadístico de estos marcadores, aportar a una base de datos colombiana y comparar con parientes de la línea masculina.Objetivo: Realizar una caracterización de haplotipos mediante análisis de marcadores moleculares, STR del cromosoma-Y en una muestra de población del altiplano cundiboyacense colombiano.Metodología: De las muestras del «Programa de Banco de muestras de sangre de funcionarios de la Fiscalía General de la Nación (FGN» fueron seleccionadas aleatoriamente 74 muestras de individuos que no reportaron relación por línea paterna entre sí y cuyo origen geográfico por línea paterna dos generaciones atrás correspondía a los departamentos de Cundinamarca o Boyacá. Se amplificaron y tipificaron 17 polimorfismos de repeticiones en tandem en el cromosoma-Y, el que se aplicó el kit comercial AmpFiSTR®YFiler™ (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438 y DYS448.Resultados: Distribución de frecuencias alélicas y haplotípicas para los 17 marcadores analizados: se determinaron 71 haplotipos con 17 marcadores STR-Y, de los que 68 eran únicos y 3 fueron observados en 2 individuos con un haplotipo compartido para los dos departamentos del altiplano cundiboyacense. La diversidad haplotípica para la muestra poblacional fue de 99.9% con un poder de discriminaci

  3. 圈C4的(St(r),St(r+1)Gr,St(r+1)-冠的优美性%On the gracefulness of the(St(r), St(r+1)Gr, St(r+1)- corona of the cycle C4

    Institute of Scientific and Technical Information of China (English)

    康芳茂; 吴跃生

    2014-01-01

    给出了圈C4的(St(r), St(r+1)Gr, St(r+1)-冠的定义,讨论了圈C4的(St(r), St(r+1)Gr, St(r+1)-冠的优美性,用构造性的方法给出了圈C4的(St(r), St(r+1)Gr, St(r+1)-冠的优美标号。%Gave the definition of the (St (r ), St (r +1), Gr , St (r +1)) - corona of the cycle C4.Discussed the gracefulness of the (St (r ), St (r +1), Gr , St (r +1)) - corona of the cycleC4.The graceful labelings was given by the method of construction.

  4. Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles

    DEFF Research Database (Denmark)

    Gelardi, Chiara; Rockenbauer, Eszter; Dalsgaard, Sigrun;

    2014-01-01

    report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed...

  5. Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China.

    Science.gov (United States)

    Fu, Xiaoliang; Fu, Yong; Liu, Ying; Guo, Juanjuan; Liu, Yanfang; Guo, Yadong; Yan, Jie; Cai, Jifeng; Liu, Jinshan; Zha, Lagabaiyila

    2016-07-01

    To study the population data of Y chromosome STR (Y-STRs) of the Mongolian minority population residing in the Horqin district, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS388, DYS449, DYS460, and YGATAH4) in 298 unrelated Chinese Mongolian individuals using the commercially available Goldeneye® DNA ID 26Y system. We also investigated blood stains, saliva spots, semen spots, hair follicles, fingernails, and sweat latent fingerprints from ten healthy males for testing the efficiency of direct amplification of this new Y-STRs system. The calculated average gene diversity values of the Mongolian population ranged from 0.3024 to 0.9510 for the DYS389I and DYS385a/b loci, respectively. The discriminatory capacity was 92.95 % with 277 observed haplotypes using 23 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and YGATAH4). By adding three more Y-STRs (DYS388, DYS449, and DYS460) to the 26Y system, the discriminatory capacity was increased to 94.63 % with a total of 282 observed haplotypes. Population relationships were calculated and compared with seven populations available from the Y chromosome haplotype reference database and data from ten Asian populations published previously. The Mongolian minority population residing in Horqin district is significantly different from other populations. Our results indicated that these 26 Y-STRs were highly genetically polymorphic in the Mongolian group and this contributes greatly to existing Chinese ethnic genetic information. As a result of direct amplification, we have obtained full profile from all blood stains, saliva spots, hair follicles, and fingernails; six semen spots; and one sweat latent fingerprint. It revealed

  6. Genetic polymorphisms of 26 Y-STR loci in the Mongolian minority from Horqin district, China.

    Science.gov (United States)

    Fu, Xiaoliang; Fu, Yong; Liu, Ying; Guo, Juanjuan; Liu, Yanfang; Guo, Yadong; Yan, Jie; Cai, Jifeng; Liu, Jinshan; Zha, Lagabaiyila

    2016-07-01

    To study the population data of Y chromosome STR (Y-STRs) of the Mongolian minority population residing in the Horqin district, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS388, DYS449, DYS460, and YGATAH4) in 298 unrelated Chinese Mongolian individuals using the commercially available Goldeneye® DNA ID 26Y system. We also investigated blood stains, saliva spots, semen spots, hair follicles, fingernails, and sweat latent fingerprints from ten healthy males for testing the efficiency of direct amplification of this new Y-STRs system. The calculated average gene diversity values of the Mongolian population ranged from 0.3024 to 0.9510 for the DYS389I and DYS385a/b loci, respectively. The discriminatory capacity was 92.95 % with 277 observed haplotypes using 23 Y-STR loci (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and YGATAH4). By adding three more Y-STRs (DYS388, DYS449, and DYS460) to the 26Y system, the discriminatory capacity was increased to 94.63 % with a total of 282 observed haplotypes. Population relationships were calculated and compared with seven populations available from the Y chromosome haplotype reference database and data from ten Asian populations published previously. The Mongolian minority population residing in Horqin district is significantly different from other populations. Our results indicated that these 26 Y-STRs were highly genetically polymorphic in the Mongolian group and this contributes greatly to existing Chinese ethnic genetic information. As a result of direct amplification, we have obtained full profile from all blood stains, saliva spots, hair follicles, and fingernails; six semen spots; and one sweat latent fingerprint. It revealed

  7. Megachilidae (Hymenoptera, Apoidea) from Malaŵi (Central Africa). The genera Creightonella, Megachile (s.str.) and Chalicodoma

    NARCIS (Netherlands)

    Schulten, G.G.M.

    1977-01-01

    A large collection of Hymenoptera was made in Malaŵi (Central Africa) between 1968 and mid 1975. In this collection there are 113 specimens (10 species) of the genus Creightonella, 408 specimens (38 species) of the genus Megachile (s.str.) and 348 specimens (12 species and 6 subspecies) of the genus

  8. Grå strækninger på det overordnede vejnet i det åbne land

    DEFF Research Database (Denmark)

    Sørensen, Michael

    2006-01-01

    Det grå strækningsarbejde er i de sidste 10 år blevet en større og større del af de danske vejbestyrelsers stedbundne trafiksikkerhedsarbejde. Imidlertid er der hverken blevet formuleret en præcis og brugbar definition af begrebet, formuleret en overordnet filosofi for arbejdet eller blevet udvik...

  9. Genetic Polymorphism of Nine Non-CODIS STR Loci in Hu-nan Province-based Chinese Han Population

    Institute of Scientific and Technical Information of China (English)

    GUO Juan-juan; LIU Ying; GUO Ya-dong; YAN Jie; CHANG Yun-feng; CAI Ji-feng; LU Ting; ZHA Lagabaiyila

    2014-01-01

    Objective To determine the allelic frequency distribution and genetic parameters of nine non-CODIS DNA index systems of the short tandemrepeat (STR ) loci (D2S1772, D6S1043, D7S3048, D8S1132, D11S2368, D12S391, D13S325, D18S1364, and GATA198B05). Methods A total of 353 blood samples were collected, extracted, amplified, and analyzed fromunrelated healthy individuals of Han na-tionality in Hunan Province, China. Results O ne hundred and fourteen alleles were observed in the pop-ulation with corresponding allelic frequencies ranged from0.001 0 to 0.323 0. For all the nine non-CODIS STR loci, the observed genotypic data showed no significant deviations fromthe Hardy-W einberg equi-librium. The Ho, He, PIC, D P, and PE of the studied non-CODIS STR loci ranged from0.108 0 to 0.195 0, 0.805 0 to 0.892 0, 0.770 0 to 0.860 0, 0.925 0 to 0.966 0 and 0.607 0 to 0.780 0, respectively. Conclusion N ine non-CODIS STR loci have high degrees of polymorphisms, which may be useful in in-dividual forensic identification and parentage testing in forensic practice.

  10. Genome-wide identification of transcriptional start sites in the plant pathogen Pseudomonas syringae pv. tomato str. DC3000

    Science.gov (United States)

    RNA-Seq has provided valuable insights into global gene expression in a number of organisms. Using a modified RNA-Seq approach and Illumina’s high-throughput sequencing technology, we globally identified 5’-ends of transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. A sub...

  11. Trådlös strömning av en ljudsignal till multipla mottagare

    OpenAIRE

    Sandberg, Johnny

    2013-01-01

    I detta examensarbete har det undersökts vilken teknik för trådlös kommunikation som passar bäst att användas då en ljudsignal ska strömmas trådlöst till multipla mottagare. De tekniker som jämfördes var Wi-Fi och Bluetooth. Andra tekniker för trådlös kommunikation, ZigBee, ANT+ och NFC, valdes bort för att deras maximala datahastighet eller räckvidd inte var tillräcklig. 3G och 4G valdes bort för att det krävs en licens för att få använda dessa. Som mottagare skulle i första hand Android- oc...

  12. Impact of a chromosome X STR Decaplex in deficiency paternity cases

    Science.gov (United States)

    Trindade-Filho, Aluisio; Ferreira, Samuel; Oliveira, Silviene F.

    2013-01-01

    Deficiency paternity cases, characterized by the absence of the alleged father, are a challenge for forensic genetics. Here we present four cases with a female child and a deceased alleged father in which the analysis of a set of 21 or 22 autosomal STRs (AS STRs) produced results within a range of doubt when genotyping relatives of the alleged father. Aiming to increase the Paternity Index (PI) and obtain more reliable results, a set of 10 X-linked STR markers, developed by the Spanish and Portuguese Group of the International Society for Forensic Genetics (ISFG), was then added. Statistical analysis substantially shifted the results towards the alleged fatherhood in all four cases, with more dramatic changes when the supposed half-sister and respective mother were the relatives tested. PMID:24385853

  13. Haplotype diversity of 17 Y-str loci in an admixed population from the Brazilian Amazon

    Science.gov (United States)

    Francez, Pablo Abdon da Costa; Ramos, Luiz Patrick Vidal; de Jesus Brabo Ferreira Palha, Teresinha; dos Santos, Sidney Emanuel Batista

    2012-01-01

    The allelic and haplotype frequencies of 17 Y-STR loci most commonly used in forensic testing were estimated in a sample of 138 unrelated healthy males from Macapá, in the northern Amazon region of Brazil. The average gene diversity was 0.6554 ± 0.3315. 134 haplotypes of the 17 loci were observed, 130 of them unique and four present in two individuals each. The haplotype diversity index was 0.9996 + 0.0009, with the most frequent haplogroups being R1b (52.2%), E1b1b (11.6%), J2 (10.1%) and Q (7.2%). Most haplogroups of this population belonged to European male lineages (89.2%), followed by Amerindian (7.2%) and African (3.6%) lineages. PMID:22481873

  14. Genetic polymorphism of 17 Y-STR loci in Han Chinese living in Lanzhou.

    Science.gov (United States)

    Sun, Hong-bing; Yang, Xin; Ha, Fei; Zhang, Zi-long

    2013-12-01

    The genetic polymorphism across 17 Y-STR loci in a population of Han Chinese in Lanzhou was investigated. Haplotypes and allele frequencies for the 17 Y-chromosomal STRs loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438 and DYS448 were determined in 500 healthy unrelated autochthonous males from Lanzhou. The results showed that no shared haplotypes were observed. Gene diversity values ranged from 0.3987 (DYS391) to 0.9740 (DYS385a,b). It was concluded that these loci will be very useful for human identification in forensic cases and paternity tests within the Han Chinese population inhabiting Lanzhou.

  15. Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast

    KAUST Repository

    Tehseen, Muhammad

    2014-09-25

    It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.

  16. Genetic polymorphism of 21 non-CODIS STR loci in the Chinese Mongolian ethnic minority.

    Science.gov (United States)

    Zha, Lagabaiyila; Liu, Ying; Guo, Yadong; Li, Jun; Wang, Ke; Geng, Kun; Liao, Qiao; Liu, Jinshan; Chen, Hanchun; Cai, Jifeng

    2014-03-01

    In this research, we investigated the allele frequencies and forensic parameters of 21 non-, CODIS short tandem repeat (STR) loci (D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435 and D5S2500) among 523 unrelated, Chinese Mongolians in the city of Tongliao, Horqin district, Inner Mongolia Autonomous Region.

  17. Genetic polymorphism of 17 Y-STR loci in Han Chinese living in Lanzhou.

    Science.gov (United States)

    Sun, Hong-bing; Yang, Xin; Ha, Fei; Zhang, Zi-long

    2013-12-01

    The genetic polymorphism across 17 Y-STR loci in a population of Han Chinese in Lanzhou was investigated. Haplotypes and allele frequencies for the 17 Y-chromosomal STRs loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438 and DYS448 were determined in 500 healthy unrelated autochthonous males from Lanzhou. The results showed that no shared haplotypes were observed. Gene diversity values ranged from 0.3987 (DYS391) to 0.9740 (DYS385a,b). It was concluded that these loci will be very useful for human identification in forensic cases and paternity tests within the Han Chinese population inhabiting Lanzhou. PMID:24337856

  18. Multiple stellar populations in the globular cluster M3 (NGC 5272): a Str\\"omgren perspective

    CERN Document Server

    Massari, Davide; Bragaglia, Angela; Dalessandro, Emanuele; Ramos, Rodrigo Contreras; Amigo, Pia

    2016-01-01

    We present Str\\"omgren photometry of the Galactic Globular Cluster M3 to study its multiple generations phenomenon. The use of different colour-magnitude diagrams and especially of the notoriously efficient c_y index allowed us to detect a double Red Giant Branch in the cluster CMD. After decontamination from fore- and background sources, the two sequences turned out to be equally populated. The two components also show a bimodal radial distribution well corresponding to that predicted by numerical simulations for clusters living in an intermediate dynamical evolutive state and with a population with modified chemical composition that was born more centrally concentrated than the primordial. The analysis of high-resolution spectra quantitatively demonstrates that the two detected sequences correspond to the first (Na-poor) generation and the second (Na-rich) generation, thus confirming the importance of synergy between photometry and spectroscopy.

  19. Geotechnical variability of permafrozen glaciomarine clays in Sdr. Strømfjord in Greenland

    DEFF Research Database (Denmark)

    Foged, Niels Nielsen; Ingeman-Nielsen, Thomas; Belmonte, Louise Josefine

    2014-01-01

    This contribution presents the geotechnical properties of some permafrozen glaciomarine clays near to the Kangerlussuaq Airport at Sdr. Strømfjord in West Greenland.This fjord system was established by glacial erosion into the bedrock consisting of Nagssugtoqidian banded gneisses with amphibolitic...... level with Upper Marine Limit (UML) varying from +120 to +140m at the West Coast to +40 at Kangerlussuaq. This retreat is well documented through C14-dating in the local area near to Kangerlussuaq Airport related to Fjord Stages F2 (+60m/8300 y BC) and F3 (+40m/8100 y BC) and Mt. Keglen stage (+40m/7200...... y BC) 5 km east of the Airport. Subformations found are; glaciomarine clay deposited in a coastal environment as very fine flocculated suspended matter ("rock flour"), deltaic sediments of silt and finesand and meltwater gravel and sand carried by the meltwater rivers. This sedimentation is still on...

  20. Characterisation of three Amerindian populations from Hidalgo State (Mexico) by 15 STR-PCR polymorphisms.

    Science.gov (United States)

    Barrot, C; Sánchez, C; Ortega, M; González-Martín, A; Brand-Casadevall, C; Gorostiza, A; Huguet, E; Corbella, J; Gené, M

    2005-03-01

    The purpose of this study is to report allele frequency data of three ethnic Amerindian population samples: the Otomi (Hna-hnu) from eastern Sierra Madre and Ixmiquilpan valley and the Huasteco from La Huasteca. These groups were characterised by 15 STR-PCR polymorphisms (HumTH01, HumvWA, D18S51, HumTPOX, D19S433, D16S539, D13S317, D8S1179, D7S820, D5S818, HumFGA, CSF1PO, D2S1338, D3S1358 and D21S11). No significant deviations in observed allelic frequencies from Hardy-Weinberg equilibrium were found for all the studied systems. From the forensic point of view, the heterozygosity value, power of discrimination and the a priori chance of exclusion were calculated. PMID:15378309

  1. Forensic and population genetic analyses of eighteen non-CODIS miniSTR loci in the Korean population.

    Science.gov (United States)

    Jin, Han Jun; Kim, Ki Cheol; Yoon, Cha Eun; Kim, Wook

    2013-11-01

    We analyzed the variation of eighteen miniSTR loci in 411 randomly chosen individuals from Korea to increase the probability that a degraded sample can be typed, as well as to provide an expanded and reliable population database. Six multiplex PCR systems were developed (multiplex I: D1S1677, D2S441 and D4S2364; multiplex II: D10S1248, D14S1434 and D22S1045; multiplex III: D12S391, D16S3253 and D20S161; multiplex IV: D3S4529, D8S1115 and D18S853; multiplex V: D6S1017, D11S4463 and D17S1301; multiplex VI: D5S2500, D9S1122 and D21S1437). Allele frequencies and forensic parameters were calculated to evaluate the suitability and robustness of these non-CODIS miniSTR systems. No significant deviation from Hardy-Weinberg equilibrium expectations were observed, except for D4S2364, D5S2500 and D20S161 loci. A multidimensional scaling plot based on allele frequencies of the six miniSTR loci (D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045) showed that Koreans appeared to have most genetic affinity with Chinese and Japanese than to other Eurasian populations compared here. The combined probability of match calculated from the 18 miniSTR loci was 2.902 × 10(-17), indicating a high degree of polymorphism. Thus, the 18 miniSTR loci can be suitable for recovering useful information for analyzing degraded forensic casework samples and for adding supplementary genetic information for a variety of analyses involving closely related individuals where there is a need for additional genetic information.

  2. Overview of European population clustering based on 23 Y-STR loci

    Directory of Open Access Journals (Sweden)

    Dogan Serkan

    2015-01-01

    Full Text Available Short tandem repeats (STRs located on the Y-chromosome are a useful tool for various scientific fields, such as forensic investigation, but also for the investigation of population structure and molecular history. In this study, population data based on 23 Y-STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643 from 23 European human populations were compared. All haplotype data for this research were gathered from previously published articles. Arlequin v3.5.1.2, POPTREE2, and MEGA 5.1 software packages were used for the calculation of allelic frequencies and genetic distance, and the construction of the European, as well as worldwide phylogenetic trees. Obtained results indicate a formation of several distinct sub-clusters within European population cluster. Observed sub-clusters were mostly recognized within geographically closer populations, meaning that neighboring populations were a part of the same sub-cluster in most of the cases. Compared with the previously published results obtained using autosomal STR markers, a significant level of concordance was detected. However, it seems that Y-STRs analyzed in this study are more informative since they enabled regional clustering in addition to continental clustering. Also, the use of a larger number of loci yielded clustering that is more specific than what has been calculated to date. Finally, it can be concluded that this study has shown that the application of a larger number of loci enables the more detailed insight into the relationships between European populations, compared to what has been published before.

  3. Analysis of 22 Y chromosomal STR haplotypes and Y haplogroup distribution in Pathans of Pakistan.

    Science.gov (United States)

    Lee, Eun Young; Shin, Kyoung-Jin; Rakha, Allah; Sim, Jeong Eun; Park, Myung Jin; Kim, Na Young; Yang, Woo Ick; Lee, Hwan Young

    2014-07-01

    We analyzed haplotypes for 22 Y chromosomal STRs (Y-STRs), including 17 Yfiler loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DY438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4) and five additional STRs (DYS388, DYS446, DYS447, DYS449 and DYS464), and Y chromosomal haplogroup distribution in 270 unrelated individuals from the Pathans residing in the Federally Administered Tribal Areas and the North-West Frontier Province of Pakistan using in-house multiplex PCR systems. Each Y-STR showed diversities ranging from 0.2506 to 0.8538, and the discriminatory capacity (DC) was 73.7% with 199 observed haplotypes using 17 Yfiler loci. By the addition of 5 Y-STRs to the Yfiler system, the DC was increased to 85.2% while showing 230 observed haplotypes. Among the additional 5 Y-STRs, DYS446, DYS447 and DYS449 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Pathans of this study showed significant differences from other Pathan populations as well as neighboring population sets. In Y-SNP analysis, a total of 12 Y chromosomal haplogroups were observed and the most frequent haplogroup was R1a1a with 49.3% frequency. To obtain insights on the origin of Pathans, the network analysis was performed for the haplogroups G and Q observed from the Pathans and the Jewish population groups including Ashkenazim and Sephardim, but little support for a Jewish origin could be found. In the present study, we report Y-STR population data in Pathans of Pakistan, and we emphasize the need for adding additional markers to the commonly used 17 Yfiler loci to achieve more improved discriminatory capacity in a population with low genetic diversity. PMID:24709582

  4. 21个非CODISSTR基因座的遗传多态性%21 non-CODIS STR loci on the genetic polymorphism

    Institute of Scientific and Technical Information of China (English)

    孙克盛; 张宏斌; 陈浩

    2012-01-01

    目的:探讨研究江浙汉族人群 21 个非 CODIS STR 基因座的遗传多态性.方法:使用 AGCU 21 + 1 STR 荧光标记复合扩增系统对江浙地区汉族的250 个无关个体的 21 个非 CODIS STR 基因座进行扩增,统计 STR 基因座的遗传学参数.结果:扩增后取得了 21 个 STR 基因座的等位基因频率分布,检测出 6、5、8、11、9、10、15、7、13、9、6、11、13、8、7、9、6、16、8、15、9 个等位基因,也获得了 21 个 STR 基因座的 H,He,DP,PM 及 PE 等的法医遗传学资料.结论:21 个非 CODIS STR 基因座具有良好的遗传多态性,适用于法医学亲权鉴定和个体识别.

  5. Authentication of newly established human esophageal squamous cell carcinoma cell line (YM-1) using short tandem repeat (STR) profiling method.

    Science.gov (United States)

    Ayyoob, Khosravi; Masoud, Khoshnia; Vahideh, Kazeminejad; Jahanbakhsh, Asadi

    2016-03-01

    Cross-contamination during or early after establishment of a new cell line could result in the worldwide spread of a misidentified cell line. Therefore, newly established cell lines need to be authenticated by a reference standard method. This study was conducted to investigate the authenticity of a newly established epithelial cell line of human esophageal squamous cell carcinoma (ESCC) called YM-1 using short tandem repeat (STR) DNA profiling method. Primary human ESCC epithelial cells were cultured from the fresh tumor tissue of an adult female patient. Growth characteristics and epithelial originality of YM-1 cells were studied. Genomic DNA was isolated from YM-1 cells harvested at passage 22 and ESCC donor tumor sample on two different days to prevent probable DNA contamination. STR profiling was performed using AmpFℓSTR® Identifiler® Plus PCR Amplification Kit. To address whether YM-1 cells undergo genetic alteration as the passage number increases, STR profiling was performed again on harvested cells at passage 51. YM-1 cells grew as a monolayer with a population doubling time of 40.66 h. Epithelial originality of YM-1 cells was confirmed using ICC/IF staining of cytokeratins AE1/AE3. The STR profile of the ESCC donor tumor sample was the same with YM-1 cells at passage 22. However, STR profile of the donor tumor sample showed an off-ladder (OL) allele in their D7S820 locus. Also, re-profiling of YM-1 cells at passage 51 showed a loss of heterozygosity (LOH) at D18S51 locus. This suggests that long-term culture of cell lines may alter their DNA profile. Comparison of the DNA fingerprinting results in DSMZ, and ATCC STR profiling databases confirmed unique identity of YM-1 cell line. This study provides an easy, fast, and reliable procedure for authentication of newly established cell lines, which helps in preventing the spread of misidentified cells and improving the reproducibility and validity of experiments, consequently.

  6. Concrete Hydration Heat Analysis for RCB Basemat Considering Solar Radiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Seong-Cheol; Son, Yong-Ki [KEPCO International Nuclear Graduate School, Ulsan (Korea, Republic of); Choi, Seong-Cheol [Chung-Ang University, Seoul (Korea, Republic of)

    2015-05-15

    The NPP especially puts an emphasis on concrete durability for structural integrity. It has led to higher cementitious material contents, lower water-cementitious-material ratios, and deeper cover depth over reinforcing steel. These requirements have resulted in more concrete placements that are subject to high internal temperatures. The problem with high internal temperatures is the increase in the potential for thermal cracking that can decrease concrete's long-term durability and ultimate strength. Thermal cracking negates the benefits of less permeable concrete and deeper cover by providing a direct path for corrosion-causing agents to reach the reinforcing steel. The purpose of this study is to develop how to analyze and estimate accurately concrete hydration heat of the real-scale massive concrete with wide large plane. An analysis method considering concrete placement sequence was studied and solar radiation effects on the real-scale massive concrete with wide large plane were reviewed through the analytical method. In this study, the measured temperatures at the real scale structure and the analysis results of concrete hydration heat were compared. And thermal stress analysis was conducted. Through the analysis, it was found that concrete placement duration, sequence and solar radiation effects should be considered to get the accurate concrete peak temperature, maximum temperature differences and crack index.

  7. The RCB star RY Sagittarii as a pulsating star

    Science.gov (United States)

    Lloyd Evans, T.

    1986-03-01

    Measurements of CN and C2 bandstrengths in the spectrum of RY Sagittarii in 1969 and 1970, when it was on the latter part of its return to maximum and during its subsequent stay at maximum light after the deep minimum of 1967-68, show that the bands vary in strength in the 38.6-day pulsation period. The variations follow the phasing of the B-V and U-B color curves rather than the V light curve, and must be determined largely by the photospheric temperature as in the case of the carbon-rich Cepheid V553 Centauri. This is supported by a comparison of the ranges in color and in bandstrength, though the bands are stronger at a given color than in typical class Ib supergiants.

  8. Concrete Hydration Heat Analysis for RCB Basemat Considering Solar Radiation

    International Nuclear Information System (INIS)

    The NPP especially puts an emphasis on concrete durability for structural integrity. It has led to higher cementitious material contents, lower water-cementitious-material ratios, and deeper cover depth over reinforcing steel. These requirements have resulted in more concrete placements that are subject to high internal temperatures. The problem with high internal temperatures is the increase in the potential for thermal cracking that can decrease concrete's long-term durability and ultimate strength. Thermal cracking negates the benefits of less permeable concrete and deeper cover by providing a direct path for corrosion-causing agents to reach the reinforcing steel. The purpose of this study is to develop how to analyze and estimate accurately concrete hydration heat of the real-scale massive concrete with wide large plane. An analysis method considering concrete placement sequence was studied and solar radiation effects on the real-scale massive concrete with wide large plane were reviewed through the analytical method. In this study, the measured temperatures at the real scale structure and the analysis results of concrete hydration heat were compared. And thermal stress analysis was conducted. Through the analysis, it was found that concrete placement duration, sequence and solar radiation effects should be considered to get the accurate concrete peak temperature, maximum temperature differences and crack index

  9. A multifluorescent STR-PCR for prenatal gene diagnosis of hemophilia A carriers%多重荧光STR-PCR方法在血友病A携带者产前基因诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    李少英; 马晓燕; 张慧敏; 王晓蔓; 黎青

    2011-01-01

    Objective To establish a reliable method for prenatal diagnosis of hemophilia A hy genetic linkage analysis. Method Multifluorescent STR-PCR method was used to analyze 50 normal females and 20 hemophilia A families. Result The heterozygote rate of DXS15 , DXS9901 , G6PD , DXS1073 , DXS1108 and F8Civs13 were 88% , 84% , 20o/e , 62% , 22% and 30%. Twenty hemophilia A carriers were successfully identified by these six STRs loci. Conclusion Multifluorescent STR-PCR is a convenient and efficient method for prenatal diagnosis of hemophilia A.%目的:通过血友病A家系遗传连锁分析,建立血友病A携带者产前诊断方法.方法:采用多重荧光STR-PCR方法对50例正常女性进行检测和20个血友病A家系进行连锁分析.结果:DXS15、DXS9901、G6PD、DXS1073、DXS1108和F8Civs13的杂合率分别为88%、84%、20%、62%、22%和30%;用这6个位点提供的遗传信息成功为20个血友病A携带者进行了产前诊断.结论:多重荧光STR-PCR方法是一种快速、简便、实用的血友病A产前诊断方法.

  10. CRED Subsurface Temperature Recorder (STR); NWHI, FFS; Long: -166.26197, Lat: 23.76887 (WGS84); Sensor Depth: 10.10m; Data Range: 20100515-20100909.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); CNMI, MAU; Long: 145.20714, Lat: 20.01792 (WGS84); Sensor Depth: 31.39m; Data Range: 20070531-20080516.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.15112, Lat: 20.86452 (WGS84); Sensor Depth: 14.63m; Data Range: 20060819-20080918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); NWHI, NEC; Long: -164.69775, Lat: 23.57152 (WGS84); Sensor Depth: 17.07m; Data Range: 20050414-20060904.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.86506, Lat: 27.94428 (WGS84); Sensor Depth: 35.66m; Data Range: 20080923-20100915.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); NWHI, GAR; Long: -167.99952, Lat: 24.99883 (WGS84); Sensor Depth: 10.40m; Data Range: 20030719-20040919.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); CNMI, SAR; Long: 145.76892, Lat: 16.71057 (WGS84); Sensor Depth: 1.00m; Data Range: 20030824-20050918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); PRIA, JOH; Long: -169.55502, Lat: 16.71490 (WGS84); Sensor Depth: 3.04m; Data Range: 20060121-20080127.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); NWHI, LIS; Long: -173.99440, Lat: 25.98698 (WGS84); Sensor Depth: 14.33m; Data Range: 20060928-20081005.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); CNMI, GUA; Long: 144.70392, Lat: 13.24217 (WGS84); Sensor Depth: 4.88m; Data Range: 20070515-20070910.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.73108, Lat: 27.86608 (WGS84); Sensor Depth: 21.95m; Data Range: 20070809-20080924.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.86350, Lat: 27.94233 (WGS84); Sensor Depth: 24.38m; Data Range: 20070806-20080923.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.83137, Lat: 27.89797 (WGS84); Sensor Depth: 0.91m; Data Range: 20080924-20100916.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); PRIA, JAR; Long: -160.00822, Lat: -00.36895 (WGS84); Sensor Depth: 32.00m; Data Range: 20080328-20100401.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); AMSM, ROS; Long: -168.16016, Lat: -14.55127 (WGS84); Sensor Depth: 3.40m; Data Range: 20080312-20090714.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); PRIA, BAK; Long: -176.48881, Lat: 00.19171 (WGS84); Sensor Depth: 16.80m; Data Range: 20040124-20050627.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.26707, Lat: 21.08087 (WGS84); Sensor Depth: 18.90m; Data Range: 20050802-20060729.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); PRIA, JOH; Long: -169.48513, Lat: 16.74080 (WGS84); Sensor Depth: 2.10m; Data Range: 20040116-20060120.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); AMSM, ROS; Long: -168.16025, Lat: -14.55134 (WGS84); Sensor Depth: 9.14m; Data Range: 20060308-20080312.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); MHI, LAN; Long: -156.83705, Lat: 20.87186 (WGS84); Sensor Depth: 13.11m; Data Range: 20081019-20101021.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); MHI, LAN; Long: -156.87525, Lat: 20.74163 (WGS84); Sensor Depth: 11.58m; Data Range: 20081020-20101022.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); MHI, LAN; Long: -156.83701, Lat: 20.87187 (WGS84); Sensor Depth: 13.10m; Data Range: 20060805-20081019.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); MHI, LAN; Long: -156.96869, Lat: 20.73729 (WGS84); Sensor Depth: 8.23m; Data Range: 20050804-20060806.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.38440, Lat: 06.38252 (WGS84); Sensor Depth: 6.40m; Data Range: 20060330-20080404.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.34216, Lat: 06.39241 (WGS84); Sensor Depth: 6.71m; Data Range: 20080405-20100414.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.38524, Lat: 06.40218 (WGS84); Sensor Depth: 10.06m; Data Range: 20080405-20100414.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.37740, Lat: 06.38552 (WGS84); Sensor Depth: 3.35m; Data Range: 20060330-20080404.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.35929, Lat: 06.38268 (WGS84); Sensor Depth: 6.40m; Data Range: 20080404-20100414.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); PRIA, KIN; Long: -162.38180, Lat: 06.42888 (WGS84); Sensor Depth: 5.18m; Data Range: 20080407-20100415.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.40177, Lat: 28.19357 (WGS84); Sensor Depth: 1.22m; Data Range: 20060915-20080926.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); PRIA, WAK; Long: 166.63805, Lat: 19.30092 (WGS84); Sensor Depth: 1.22m; Data Range: 20070505-20090324.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.32340, Lat: 28.24453 (WGS84); Sensor Depth: 0.91m; Data Range: 20041003-20060915.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.38602, Lat: 28.27105 (WGS84); Sensor Depth: 1.52m; Data Range: 20041010-20060915.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.40181, Lat: 28.19361 (WGS84); Sensor Depth: 1.00m; Data Range: 20030729-20041001.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.36784, Lat: 28.27763 (WGS84); Sensor Depth: 0.30m; Data Range: 20030729-20041001.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.36784, Lat: 28.27774 (WGS84); Sensor Depth: 0.30m; Data Range: 20020926-20030727.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.37492, Lat: 28.19637 (WGS84); Sensor Depth: 14.02m; Data Range: 20060916-20080925.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.36787, Lat: 28.27767 (WGS84); Sensor Depth: 0.91m; Data Range: 20041012-20060915.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.36784, Lat: 28.27764 (WGS84); Sensor Depth: 1.22m; Data Range: 20060915-20080925.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.40178, Lat: 28.19358 (WGS84); Sensor Depth: 0.91m; Data Range: 20041002-20050520.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); NWHI, MID; Long: -177.32334, Lat: 28.24437 (WGS84); Sensor Depth: 0.50m; Data Range: 20030729-20041001.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.30621, Lat: 28.44759 (WGS84); Sensor Depth: 0.91m; Data Range: 20080929-20100918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.32575, Lat: 28.38175 (WGS84); Sensor Depth: 12.50m; Data Range: 20090915-20100519.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.36842, Lat: 28.42927 (WGS84); Sensor Depth: 0.60m; Data Range: 20020925-20030802.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.34457, Lat: 28.41858 (WGS84); Sensor Depth: 9.75m; Data Range: 20041024-20060917.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.28268, Lat: 28.39066 (WGS84); Sensor Depth: 19.20m; Data Range: 20060918-20080111.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.30603, Lat: 28.44740 (WGS84); Sensor Depth: 0.80m; Data Range: 20030805-20041008.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.30620, Lat: 28.44760 (WGS84); Sensor Depth: 1.22m; Data Range: 20060918-20080929.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.36847, Lat: 28.42927 (WGS84); Sensor Depth: 0.91m; Data Range: 20080930-20100918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.36841, Lat: 28.42923 (WGS84); Sensor Depth: 1.22m; Data Range: 20060918-20080930.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.34453, Lat: 28.41852 (WGS84); Sensor Depth: 10.06m; Data Range: 20060917-20080929.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.28266, Lat: 28.39062 (WGS84); Sensor Depth: 19.20m; Data Range: 20080930-20100919.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.36842, Lat: 28.42927 (WGS84); Sensor Depth: 0.60m; Data Range: 20030805-20041006.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.34317, Lat: 28.41816 (WGS84); Sensor Depth: 9.75m; Data Range: 20090916-20100918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.30608, Lat: 28.44740 (WGS84); Sensor Depth: 0.80m; Data Range: 20020928-20030802.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.34327, Lat: 28.41817 (WGS84); Sensor Depth: 10.06m; Data Range: 20080929-20090916.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.35619, Lat: 28.45157 (WGS84); Sensor Depth: 18.59m; Data Range: 20060918-20080930.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.36845, Lat: 28.42927 (WGS84); Sensor Depth: 0.61m; Data Range: 20041008-20060917.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. Genetic variability and phylogenetic analysis of Han population from Guanzhong region of China based on 21 non-CODIS STR loci.

    Science.gov (United States)

    Zhang, Yu-Dang; Tang, Xiao-Li; Meng, Hao-Tian; Wang, Hong-Dan; Jin, Rui; Yang, Chun-Hua; Yan, Jiang-Wei; Yang, Guang; Liu, Wen-Juan; Shen, Chun-Mei; Zhu, Bo-Feng

    2015-01-01

    In the present study, we presented the population genetic data and their forensic parameters of 21 non-CODIS autosomal STR loci in Chinese Guanzhong Han population. A total of 166 alleles were observed with corresponding allelic frequencies ranging from 0.0018 to 0.5564. No STR locus was observed to deviate from the Hardy-Weinberg equilibrium and linkage disequilibriums after applying Bonferroni correction. The cumulative power of discrimination and probability of exclusion of all the 21 STR loci were 0.99999999999999999993814 and 0.999998184, respectively. The results of genetic distances, phylogenetic trees and principal component analysis revealed that the Guanzhong Han population had a closer relationship with Ningxia Han, Tujia and Bai groups than other populations tested. In summary, these 21 STR loci showed a high level of genetic polymorphisms for the Guanzhong Han population and could be used for forensic applications and the studies of population genetics.

  9. CRED Subsurface Temperature Recorder (STR); NWHI, LAY; Long: -171.72941, Lat: 25.75893 (WGS84); Sensor Depth: 0.91m; Data Range: 20040924-20060730.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); PRIA, JAR; Long: -159.97426, Lat: -00.37555 (WGS84); Sensor Depth: 12.80m; Data Range: 20080327-20100403.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.64297, Lat: 21.01736 (WGS84); Sensor Depth: 12.19m; Data Range: 20081023-20101018.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.42031, Lat: 20.59198 (WGS84); Sensor Depth: 14.90m; Data Range: 20060805-20071009.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.58448, Lat: 20.79079 (WGS84); Sensor Depth: 11.89m; Data Range: 20081024-20100326.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.42031, Lat: 20.59198 (WGS84); Sensor Depth: 14.90m; Data Range: 20071009-20081018.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.15120, Lat: 20.86456 (WGS84); Sensor Depth: 14.63m; Data Range: 20081018-20101016.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.42033, Lat: 20.59195 (WGS84); Sensor Depth: 14.63m; Data Range: 20081018-20101020.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.49718, Lat: 20.63030 (WGS84); Sensor Depth: 11.58m; Data Range: 20050807-20101017.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); MHI, MAI; Long: -156.58458, Lat: 20.79070 (WGS84); Sensor Depth: 14.60m; Data Range: 20050806-20070402.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); NWHI, KUR; Long: -178.35620, Lat: 28.45155 (WGS84); Sensor Depth: 20.12m; Data Range: 20081001-20100918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.26699, Lat: 21.08091 (WGS84); Sensor Depth: 20.12m; Data Range: 20081025-20101023.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); PRIA, PAL; Long: -162.06183, Lat: 05.88278 (WGS84); Sensor Depth: 4.26m; Data Range: 20060324-20080330.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); AMSM, OFU; Long: -169.65265, Lat: -14.17770 (WGS84); Sensor Depth: 1.22m; Data Range: 20080228-20100311.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); CNMI, FDP; Long: 144.89860, Lat: 20.53790 (WGS84); Sensor Depth: 11.28m; Data Range: 20070603-20090427.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); PRIA, JAR; Long: -159.99110, Lat: -00.36323 (WGS84); Sensor Depth: 9.80m; Data Range: 20040328-20060320.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); AMSM, SWA; Long: -171.06473, Lat: -11.05254 (WGS84); Sensor Depth: 15.24m; Data Range: 20080316-20100317.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); PRIA, PAL; Long: -162.08592, Lat: 05.88624 (WGS84); Sensor Depth: 3.70m; Data Range: 20040402-20060325.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); CNMI, FDP; Long: 144.88580, Lat: 20.54513 (WGS84); Sensor Depth: 12.50m; Data Range: 20070603-20070826.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); CNMI, TIN; Long: 145.62162, Lat: 15.08075 (WGS84); Sensor Depth: 12.80m; Data Range: 20070519-20090412.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); NWHI, LIS; Long: -173.99799, Lat: 26.10010 (WGS84); Sensor Depth: 22.56m; Data Range: 20081004-20090929.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); NWHI, LIS; Long: -173.88422, Lat: 25.94284 (WGS84); Sensor Depth: 20.42m; Data Range: 20060927-20081004.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); CNMI, PAG; Long: 145.75748, Lat: 18.12728 (WGS84); Sensor Depth: 5.79m; Data Range: 20070605-20080513.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); NWHI, FFS; Long: -166.27505, Lat: 23.85625 (WGS84); Sensor Depth: 7.62m; Data Range: 20070924-20080915.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); PRIA, PAL; Long: -162.04044, Lat: 05.87450 (WGS84); Sensor Depth: 5.18m; Data Range: 20080402-20100409.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); NWHI, LIS; Long: -173.88414, Lat: 25.94299 (WGS84); Sensor Depth: 21.34m; Data Range: 20081004-20100922.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); NWHI, MAR; Long: -170.63378, Lat: 25.44642 (WGS84); Sensor Depth: 8.23m; Data Range: 20060907-20080918.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); MHI, HAW; Long: -155.68428, Lat: 18.92253 (WGS84); Sensor Depth: 12.80m; Data Range: 20060803-20081101.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); NWHI, FFS; Long: -166.17377, Lat: 23.64515 (WGS84); Sensor Depth: 1.83m; Data Range: 20080915-20100910.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); AMSM, OFU; Long: -169.65947, Lat: -14.18290 (WGS84); Sensor Depth: 1.50m; Data Range: 20040825-20060505.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); MHI, KAU; Long: -159.29895, Lat: 22.16533 (WGS84); Sensor Depth: 14.94m; Data Range: 20050715-20060808.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); PRIA, JAR; Long: -159.97228, Lat: -00.37500 (WGS84); Sensor Depth: 30.70m; Data Range: 20060320-20080328.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); AMSM, OFU; Long: -169.65219, Lat: -14.18017 (WGS84); Sensor Depth: 6.10m; Data Range: 20080228-20100311.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); MHI, FFP; Long: -160.60315, Lat: 21.68671 (WGS84); Sensor Depth: 16.40m; Data Range: 20060811-20080605.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); MHI, FFP; Long: -160.60318, Lat: 21.68678 (WGS84); Sensor Depth: 18.90m; Data Range: 20081111-20100928.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.83968, Lat: 27.78481 (WGS84); Sensor Depth: 23.16m; Data Range: 20070806-20081002.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); NWHI, LIS; Long: -173.99792, Lat: 26.10018 (WGS84); Sensor Depth: 23.16m; Data Range: 20060926-20081004.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.88112, Lat: 27.78202 (WGS84); Sensor Depth: 23.16m; Data Range: 20060913-20060922.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.88105, Lat: 27.78206 (WGS84); Sensor Depth: 23.16m; Data Range: 20070805-20080923.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); NWHI, FFS; Long: -166.18559, Lat: 23.63498 (WGS84); Sensor Depth: 23.16m; Data Range: 20061001-20071001.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.88112, Lat: 27.78204 (WGS84); Sensor Depth: 23.16m; Data Range: 20060913-20070805.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.88098, Lat: 27.78213 (WGS84); Sensor Depth: 23.16m; Data Range: 20040929-20060913.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); MHI, OAH; Long: -158.23436, Lat: 21.53197 (WGS84); Sensor Depth: 19.20m; Data Range: 20090902-20100715.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); AMSM, ROS; Long: -168.16885, Lat: -14.54882 (WGS84); Sensor Depth: 31.09m; Data Range: 20060308-20080312.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.92635, Lat: 27.75970 (WGS84); Sensor Depth: 22.25m; Data Range: 20070805-20081002.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); CNMI, ASC; Long: 145.38974, Lat: 19.69340 (WGS84); Sensor Depth: 3.65m; Data Range: 20070604-20090425.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.25236, Lat: 21.20307 (WGS84); Sensor Depth: 10.36m; Data Range: 20081024-20101023.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); PRIA, WAK; Long: 166.59801, Lat: 19.31633 (WGS84); Sensor Depth: 24.08m; Data Range: 20051023-20070430.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); NWHI, PHR; Long: -175.89428, Lat: 27.91178 (WGS84); Sensor Depth: 2.40m; Data Range: 20030730-20040930.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); CNMI, AGR; Long: 145.63821, Lat: 18.76660 (WGS84); Sensor Depth: 1.80m; Data Range: 20050915-20070527.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); PRIA, PAL; Long: -162.08588, Lat: 05.88625 (WGS84); Sensor Depth: 3.30m; Data Range: 20060325-20071115.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); NWHI, FFS; Long: -166.26132, Lat: 23.76897 (WGS84); Sensor Depth: 3.96m; Data Range: 20040917-20060905.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); CNMI, ZEA; Long: 145.85335, Lat: 16.89749 (WGS84); Sensor Depth: 24.68m; Data Range: 20070526-20090505.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.25234, Lat: 21.20304 (WGS84); Sensor Depth: 10.30m; Data Range: 20060730-20081024.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.26707, Lat: 21.08087 (WGS84); Sensor Depth: 19.50m; Data Range: 20060730-20081025.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); MHI, MOL; Long: -157.25234, Lat: 21.20304 (WGS84); Sensor Depth: 10.36m; Data Range: 20050801-20060730.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); MHI, HAW; Long: -155.90353, Lat: 20.19118 (WGS84); Sensor Depth: 9.70m; Data Range: 20060803-20081026.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); MHI, HAW; Long: -155.90350, Lat: 20.19112 (WGS84); Sensor Depth: 9.75m; Data Range: 20050301-20060803.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); MHI, HAW; Long: -155.90350, Lat: 20.19120 (WGS84); Sensor Depth: 9.75m; Data Range: 20081027-20101008.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. Population genetics of 17 Y-STR markers in Turkish Cypriots from Cyprus.

    Science.gov (United States)

    Teralı, K; Zorlu, T; Bulbul, O; Gurkan, C

    2014-05-01

    We analyzed seventeen Y-chromosomal short tandem repeats (STRs) [DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, and DYS448] in 253 unrelated, male individuals from the Turkish Cypriot population of the Eastern Mediterranean island of Cyprus. While 206 out of the 253 haplotypes present in the dataset were unique, there are also 22 haplotypes that were observed in two individuals each, and 1 haplotype that was observed in three individuals. While no locus duplications or null alleles were observed in our dataset, we have detected 43 allelic variants in total, the majority of which (25 out of 253 haplotypes or 9.88%) comprised of .2 intermediate variants at the DYS458 locus (alleles 16.2, 17.2, 18.2, 19.2, and 20.2). For the 229 different haplotypes observed in the Turkish Cypriot dataset, the calculated discrimination capacity (DC) was 0.9051 and the haplotype diversity (HD) was 0.9992. The calculated average gene diversity (GD) values ranged from 0.3828 to 0.9631 for the DYS392 and DYS385a/b loci, respectively. Pairwise genetic distance comparisons of the Turkish Cypriot Y-STR dataset with those from the neighbouring (Turkey, Greece, Israel/Palestinian Authority area, Egypt and Italy) and relatively distant (Lithuania, Taiwan and Australia) countries through the use of analysis of molecular variance (AMOVA) and multi-dimensional scaling (MDS) analyses confirmed that our data do not deviate significantly from the typical core haplotypes of the Eastern Mediterranean region. The Turkish Cypriot Y-STR haplotype dataset will find an immediate use in the Committee on Missing Persons in Cyprus Project on the "Exhumation, Identification and Return of Remains of Missing Persons" and it is also expected to contribute to the establishment of forensic genetic services in North Cyprus. PMID:24507085

  9. Population data on 10 non-CODIS STR loci in Japanese population using a newly developed multiplex PCR system.

    Science.gov (United States)

    Asamura, H; Ota, M; Fukushima, H

    2008-11-01

    This paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 10 loci (D1S1656, D2S1353, D8S1132, D12S1090, D14S608, D18S535, D19S253, D20S480, D21S226, and D22S689) unlinked to the core STR loci (non-CODIS loci). Of 252 samples taken from the Japanese population, PCR products ranged in length from 107 bp to 319 bp. No significant deviations from Hardy-Weinberg equilibrium were observed at any of the 10 loci. The accumulated power of discrimination and power of exclusion for the 10 loci were 0.999999999998 and 0.99991, respectively. We conclude that the present multiplex system for the 10 non-CODIS loci represents a powerful tool for forensic applications.

  10. Successful DNA Profiling for Identification of burnt Families from their bones using AmpFℓSTR Identifiler® Plus Kit

    OpenAIRE

    Muhamamd Shahzad; Muhammad Shafique; Manzoor Hussain; Muhammad Adnan Shan; Rukhsana Perveen; Ziaurehman; Muhammad Idrees

    2016-01-01

    Background: DNA profiling plays a vital role in the identification of dead bodies during mass disasters. Severe fragmentation, decomposition, burning and intermixing of the remains can occur in the mass disasters. DNA analysis faces many challenges especially when the dead bodies are completely decomposed or burnt. This report presents the identification of 32 completely burnt individuals including three families from their remains in a bus using AmpFlSTR Identifiler Plus® Kit and AmpFlSTR Y-...

  11. Successful DNA Profiling for Identification of burnt Families from their bones using AmpFℓSTR Identifiler® Plus Kit

    Directory of Open Access Journals (Sweden)

    Muhamamd Shahzad

    2016-02-01

    Full Text Available Background: DNA profiling plays a vital role in the identification of dead bodies during mass disasters. Severe fragmentation, decomposition, burning and intermixing of the remains can occur in the mass disasters. DNA analysis faces many challenges especially when the dead bodies are completely decomposed or burnt. This report presents the identification of 32 completely burnt individuals including three families from their remains in a bus using AmpFlSTR Identifiler Plus® Kit and AmpFlSTR Y-filer® Kit. Methods: DNA was extracted from provided remains of burnt bodies and reference samples by organic extraction procedure. The extracted quantity of DNA was calculated on ABI SDS7500 real time PCR with Quantifiler® Human DNA Quantification Kit (Applied Biosystems. DNA samples of 32 completely burnt individuals including three families were amplified using AmpFlSTR Identifiler Plus® Kit and AmpFlSTR Y-filer® Kit. The genotyping of these amplified samples was performed on ABI 3130xl Genetic Analyzer. Results: The resulting data obtained from Genetic Analyzer was analyzed using GeneMapper ID software version 3.2 (Applied Biosystems. Seventeen burnt individuals including 3 burnt families were identified with the help of 16 autosomal STRs and 6 were identified through Y-STR analysis by allele sharing of their provided reference samples of parents and brothers respectively. Conclusion: For the identification of unknown individuals particularly burnt deceased victims, STR analysis has become the gold standard in forensic science. Successful DNA profiling through the amplification of STR markers of AmpFlSTR Identifiler Plus® Kit proved to be very helpful in identifying the remains of burnt individuals even in the presence of inhibition observed in the Real Time PCR.

  12. 24 Y-chromosomal STR haplotypic structure for Chinese Kazak ethnic group and its genetic relationships with other groups.

    Science.gov (United States)

    Mei, Ting; Zhang, Li-Ping; Liu, Yao-Shun; Chen, Jian-Gang; Meng, Hao-Tian; Yan, Jiang-Wei; Zhu, Bo-Feng

    2016-09-01

    The Kazak ethnic minority is a large ethnic group in the Xinjiang Uygur Autonomous Region of China and is valuable resource for the study of ethnogeny. In the present study, 24 Y-chromosomal short tandem repeat (Y-STR) loci were analyzed in 201 unrelated Kazak male individuals from Ili Kazak Autonomous Prefecture, Xinjiang, China. The gene diversity of the 24 Y-STR loci in the studied Kazak group ranged from 0.0050 to 0.9104. According to haplotypic analysis of the 24 Y-STR loci, 113 different haplotypes were obtained, 96 of which were unique. The haplotype diversity and discrimination capacity in Kazak group were 0.9578 and 0.5622 at 24 STR loci, respectively. The haplotype diversity and discrimination capacity at Y-filer 17 loci, extended 11 loci, and minimal 9 loci were reduced to 0.9274 and 0.4279, 0.8459 and 0.3284, and 0.8354 and 0.2985, respectively, which could indicate that the more loci were detected, the higher forensic efficacy was obtained. We evaluated the application value of the 24 loci in forensic sciences and analyzed interpopulation differentiations by making comparisons between the Kazak1 (represent our samples from Ili Kazak Autonomous Prefecture) group and other 14 groups. The results of pairwise genetic distances, multidimensional scaling plot, and neighbor-joining tree at the same set of 17 Y-filer loci indicated that the Kazak1 group had the closer genetic relationships with Kazak2 (represent samples from the whole territory of Xinjiang Uygur Autonomous Region), Mongolian, and Uygur ethnic groups. The present results may provide useful information for paternal lineages in forensic cases and can also increase our understanding of the genetic relationships between Kazak1 and other groups.

  13. Development of Chinese Forensic Y-STR DNA Database%中国Y-STR数据库建设相关问题探讨

    Institute of Scientific and Technical Information of China (English)

    葛建业; 严江伟; 谢群; 孙宏钰; 同怀谷; 李斌

    2013-01-01

    Y染色体是男性所特有的父系遗传染色体,Y染色体上的STR基因座已经大量应用于实际案件.本文总结了Y-STR的特性以及选择适合中国人群的Y-STR基因座所需要考虑的问题,展望了Y-STR在家系排查、亲缘搜索、来源人群推断、混合样本检验、亲缘关系鉴定等方面的应用前景,并探讨了Y-STR试剂盒研发、验证,Y-STR突变率,搜索软件等方面的问题,提出了相应的建议.%Y chromosome is a male-specific paternal inherited chromosome.The STR markers on Y chromosome have been widely used in forensic practices.This article summarizes the characteristics of Y-STR and some factors are considered of selecting appropriate Y-STR markers for Chinese population.The prospects of existing and potential forensic applications of Y-STR profiles are discussed including familial excluding,familial searching,crowd source deducing,mixture sample testing,and kinship identifying.The research,development,verification of Y-STR kit,Y-STR mutation rate,and search software are explored and some suggestions are given.

  14. [Analysis of genetic diversity of Russian regional populations based on common STR markers used in DNA identification].

    Science.gov (United States)

    Pesik, V Yu; Fedunin, A A; Agdzhoyan, A T; Utevska, O M; Chukhraeva, M I; Evseeva, I V; Churnosov, M I; Lependina, I N; Bogunov, Yu V; Bogunova, A A; Ignashkin, M A; Yankovsky, N K; Balanovska, E V; Orekhov, V A; Balanovsky, O P

    2014-06-01

    We conducted the first genetic analysis of a wide a range of rural Russian populations in European Russia with a panel of common DNA markers commonly used in criminalistics genetic identification. We examined a total of 647 samples from indigenous ethnic Russian populations in Arkhangelsk, Belgorod, Voronezh, Kursk, Rostov, Ryazan, and Orel regions. We employed a multiplex genotyping kit, COrDIS Plus, to genotype Short Tandem Repeat (STR) loci, which included the genetic marker panel officially recommended for DNA identification in the Russian Federation, the United States, and the European Union. In the course of our study, we created a database of allelic frequencies, examined the distribution of alleles and genotypes in seven rural Russian populations, and defined the genetic relationships between these populations. We found that, although multidimensional analysis indicated a difference between the Northern gene pool and the rest of the Russian European populations, a pairwise comparison using 19 STR markers among all populations did not reveal significant differences. This is in concordance with previous studies, which examined up to 12 STR markers of urban Russian populations. Therefore, the database of allelic frequencies created in this study can be applied for forensic examinations and DNA identification among the ethnic Russian population over European Russia. We also noted a decrease in the levels of heterozygosity in the northern Russian population compared to ethnic populations in southern and central Russia, which is consistent with trends identified previously using classical gene markers and analysis of mitochondrial DNA.

  15. Genetic polymorphism analyses of a novel panel of 19 X-STR loci in the Chinese Uygur ethnic minority* #

    Science.gov (United States)

    Guo, Yu-xin; Chen, Jian-gang; Wang, Yan; Yan, Jiang-wei; Chen, Jing; Yao, Tian-hua; Zhang, Li-ping; Yang, Guang; Meng, Hao-tian; Zhang, Yu-dang; Mei, Ting; Liu, Yao-shun; Dong, Qian; Zhu, Bo-feng

    2016-01-01

    The population genetic data and forensic parameters of 19 X-chromosome short tandem repeat (X-STR) loci in Chinese Uygur ethnic minority are presented. These loci were detected in a sample of 233 (94 males and 139 females) unrelated healthy individuals. We observed 238 alleles at the 19 X-STR loci, with the corresponding gene frequencies spanning the range from 0.0021 to 0.5644. After Bonferroni correction (P>0.0026), there were no significant deviations from Hardy-Weinberg equilibrium. The cumulative power of discrimination in females and males, and the probability of exclusion of the 19 X-STR loci were 0.999 999 999 999 999 999 998 091, 0.999 999 999 999 966, and 0.999 999 986 35, respectively. The cumulative mean exclusion chance was 0.999 999 992 849 in deficiency cases, 0.999 999 999 999 628 in normal trios, and 0.999 999 998 722 in duo cases. The high value of the forensic parameters mentioned above revealed that the novel panel of 19 loci had important values for forensic applications in the Uygur group. PMID:27143264

  16. The development and application of a multiplex short tandem repeat (STR) system for identifying subspecies, individuals and sex in tigers.

    Science.gov (United States)

    Zou, Zheng-Ting; Uphyrkina, Olga V; Fomenko, Pavel; Luo, Shu-Jin

    2015-07-01

    Poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. Development of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. We developed a multiplex genotyping system "tigrisPlex" to simultaneously assess 22 short tandem repeat (STR, or microsatellite) loci and a gender-identifying SRY gene, all amplified in 4 reactions using as little as 1 ng of template DNA. With DNA samples used for between-run calibration, the system generates STR genotypes that are directly compatible with voucher tiger subspecies genetic profiles, hence making it possible to identify subspecies via bi-parentally inherited markers. We applied "tigrisPlex" to 12 confiscated specimens from Russia and identified 6 individuals (3 females and 3 males), each represented by duplicated samples and all designated as Amur tigers (Panthera tigris altaica) with high confidence. This STR multiplex system can serve as an effective and versatile approach for genetic profiling of both wild and captive tigers as well as confiscated tiger products, fulfilling various conservation needs for identifying the origins of tiger samples. PMID:25950598

  17. [Analysis of genetic diversity of Russian regional populations based on common STR markers used in DNA identification].

    Science.gov (United States)

    Pesik, V Yu; Fedunin, A A; Agdzhoyan, A T; Utevska, O M; Chukhraeva, M I; Evseeva, I V; Churnosov, M I; Lependina, I N; Bogunov, Yu V; Bogunova, A A; Ignashkin, M A; Yankovsky, N K; Balanovska, E V; Orekhov, V A; Balanovsky, O P

    2014-06-01

    We conducted the first genetic analysis of a wide a range of rural Russian populations in European Russia with a panel of common DNA markers commonly used in criminalistics genetic identification. We examined a total of 647 samples from indigenous ethnic Russian populations in Arkhangelsk, Belgorod, Voronezh, Kursk, Rostov, Ryazan, and Orel regions. We employed a multiplex genotyping kit, COrDIS Plus, to genotype Short Tandem Repeat (STR) loci, which included the genetic marker panel officially recommended for DNA identification in the Russian Federation, the United States, and the European Union. In the course of our study, we created a database of allelic frequencies, examined the distribution of alleles and genotypes in seven rural Russian populations, and defined the genetic relationships between these populations. We found that, although multidimensional analysis indicated a difference between the Northern gene pool and the rest of the Russian European populations, a pairwise comparison using 19 STR markers among all populations did not reveal significant differences. This is in concordance with previous studies, which examined up to 12 STR markers of urban Russian populations. Therefore, the database of allelic frequencies created in this study can be applied for forensic examinations and DNA identification among the ethnic Russian population over European Russia. We also noted a decrease in the levels of heterozygosity in the northern Russian population compared to ethnic populations in southern and central Russia, which is consistent with trends identified previously using classical gene markers and analysis of mitochondrial DNA. PMID:25715463

  18. Evaluation and In-House Validation of Five DNA Extraction Methods for PCR-based STR Analysis of Bloodstained Denims

    Directory of Open Access Journals (Sweden)

    Henry Perdigon

    2004-06-01

    Full Text Available One type of crime scene evidence commonly submitted for analysis is bloodstain on denim. However, chemicals (e.g., indigo used to produce denim materials may co-purify with DNA and hence, affect subsequent DNA analysis. The present study compared five methods (e.g., standard organic, organic with hydrogen peroxide (H2O2, modified FTA™, organic/Chelex®-Centricon®, and QIAamp® DNA Mini Kit-based procedures for the isolation of blood DNA from denim. A Short Tandem Repeat (STR-based analysis across two to nine STR markers, namely, HUMvWA, HUMTH01, D8S306, HUMFES/FPS, HUMDHFRP2, HUMF13A01, HUMFGA, HUMTPOX, and HUMCSF1PO, was used to evaluate successful amplification of blood DNA extracted from light indigo, dark indigo, indigo-sulfur, pure indigo, sulfur-top, and sulfur-bottom denim materials. The results of the present study support the utility of organic/Chelex®-Centricon® and QIAamp® Kit procedures in extracting PCR-amplifiable DNA from five different types of denim materials for STR analysis. Furthermore, a solid-based method using FTA™ classic cards was modified to provide a simple, rapid, safe, and cost-effective procedure for extracting blood DNA from light, dark indigo and pure indigo denim materials. However, DNA eluted from bloodstained sulfur-dyed denims (e.g., sulfur-top and sulfur-bottom using FTA™ procedure was not readily amplifiable.

  19. Population genetics for 23 Y-STR loci in Tibetan in China and confirmation of DYS448 null allele.

    Science.gov (United States)

    Ye, Yi; Gao, Jingshang; Fan, Guangyao; Liao, Linchuan; Hou, Yiping

    2015-05-01

    Tibetan is one of 56 ethnic groups in China, where a level of genetic sub-structure might be expected. Although a global analysis of Y-chromosomal haplotype diversity for 23 STR loci and Y-STR databases with PPY23 kit were created with collaborative effort, there was a lack of data for Tibetan population. In this study we evaluated 248 unrelated male individuals of Chinese Tibetan living in the Tibet Autonomous Region to explore the underlying genetic structure of Tibetan populations. These samples were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) by using PPY23 kit. A total of 224 different haplotypes were found. Haplotype diversity was 0.9990. Both Rst pairwise analyses and multidimensional scaling plot showed the genetic structure of Tibetan population was significantly different from some of Chinese ethnic groups and neighboring populations. There were few interesting null features at DYS448 observed by PPY23 that deserved some comment. It revealed that PPY23 marker set provided substantially stronger discriminatory power in Tibetan population. PMID:25524635

  20. SEO optimalizace a E-marketing internetových stránek

    OpenAIRE

    Peťovský, Vojtěch

    2012-01-01

    Bakalářská práce se zaměřuje na dva problémy. První část se zabývá webovou prezentací a návrhem její SEO optimalizace. Druhá část se zaměřuje na její nadstavbu: marketing na internetu neboli: e-marketing. Výstupem práce má být poznatek, jak velký účinek má návrh SEO optimalizace a e-marketingu pro zvýšení počtu návštěvníků webových stránek a tím i zvýšení obratu firmy. Tyto 2 návrhy jsou nakonec zhodnoceny z ekonomického hlediska.

  1. A novel approach in personal identification from tissue samples undergone different processes through STR typing.

    Science.gov (United States)

    Staiti, N; Di Martino, D; Saravo, L

    2004-12-01

    Short tandem repeats (STRs) or microsatellites have been recognized worldwide as a powerful tool for human identification. They have become widely used in human identification especially in criminal cases and mass disasters. Police departments are often interested in cases where tissues are already decomposed and only do bones remain to let them perform laboratory analyses. Bone is the most resistant tissue in animal body to time depending degradation and putrefaction, but it is often hard to extract DNA from it because of its highly mineralized structure, which makes DNA extraction and/or amplification hard to carry out. We have performed human nuclear DNA extraction and STR typing in three different cases, on bones and bone fragments from long time dead persons found buried, in the sea, almost completely burnt and whose tissues were already decomposed. We report these caseworks as we would like to show how forensic scientists are improving their skill in identifying people whose corps have undergone several kinds of processes, even independently on the time passed and the level of putrefaction of their tissues.

  2. A fluorescent microscopy-screening test for efficient STR-typing of telogen hair roots.

    Science.gov (United States)

    Bourguignon, Luc; Hoste, Bernadette; Boonen, Tom; Vits, Kathy; Hubrecht, Françoise

    2008-12-01

    Nuclear DNA-profiling from human hairs is a well-known technique in forensic investigations, but its success rate is quite low with some hair types. As nuclear DNA (nuDNA) extracted from telogen hair roots is in short supply and is often degraded, a simple and effective method of estimating the number of nuclear DNAs in telogen roots has been developed. DAPI, a fluorescent, non-destructive DNA stain, allows the visualization of "nuclei" (DAPI-positive spots the shape and size of the human follicular cell nuclei) and does not interfere with subsequent PCR analyses. We stained 3242 telogen roots from 27 donors. Surprisingly, of the 2572 club roots without any soft tissue remnants 11% contained visible "nuclei" and 3.3% even contained many. At the same time 57% of the 670 telogen roots with soft tissue remnants did not show any fluorescent "nuclei". We analysed the STR-profile of some of the roots selected by the DAPI screening, i.e. 132 telogen roots without soft tissue remnants, with a success rate of 79%. Our proposed screening method allows the DNA laboratory to analyse nuclear DNA only in the most promising hair roots.

  3. Genetic differentiation of the Cabo Verde archipelago population analysed by STR polymorphisms.

    Science.gov (United States)

    Fernandes, A T; Velosa, R; Jesus, J; Carracedo, A; Brehm, A

    2003-07-01

    Allele frequencies for 17 STR loci were analyzed in a sample of unrelated males from the Cabo Verde Archipelago. The samples were gathered in such a way that the origin of the subjects was perfectly identified, and they could be included in one of the leeward or windward groups of islands. This study reveals that there are significant differences between both groups of islands, and between Cabo Verdeans and other populations from sub-Sahara Africa including the Guineans, the most probable source population for Cabo Verdeans. This study confirms mtDNA data and, together with HLA and Y chromosome data already published, shows that the Cabo Verde population is sub-structured and atypical, diverging substantially from mainland sub-Saharan populations. Overall these differences are most probably due to admixture between sub-Saharan slaves brought into the islands and other settlers of European origin. In the absence of a clear indication of a different ethnic composition of the first sub-Saharan settlers of Cabo Verde, the differentiation exhibited in both groups of islands can be most probably be attributed to genetic drift. PMID:12914568

  4. Allele Frequencies of 10 Autosomal STR Loci from Chakma and Tripura Tribal Populations in Bangladesh

    Directory of Open Access Journals (Sweden)

    Ahmad Ferdous

    2010-01-01

    Full Text Available Allele frequencies of ten autosomal STR loci, D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, and FGA were investigated in Chakma and Tripura tribal populations of Bangladesh. In both the populations, all loci were in Hardy-Weinberg equilibrium except for FGA locus in Chakma and D21S11 in Tripura. All the loci were highly polymorphic in Chakma population with an observed heterozygosity (Ho of >0.7 and moderately polymorphic in Tripura population (Ho>0.6. However, both the population showed least polymorphism at TH01 locus (Ho<0.6. A comparison between Chakma and Tripura population data revealed statistically significant differences in allele frequency distribution for most of the loci. A similar comparison with the mainstream Bengali population using previously published data from this lab also showed significant difference in allele frequency with these two tribal populations.

  5. Effects of heavy metal pollution on red wood ant (Formica s. str.) populations

    International Nuclear Information System (INIS)

    We studied the species composition, mound population densities, relative abundance and colony sizes of red wood ants along a well known air pollution gradient of a copper smelter in Southwest Finland. The dominant species, Formica aquilonia, was further studied for heavy metal (Al, Cu, Cd, Ni, Zn, As, Pb, Hg) levels and morphological characters (body mass, head width, labial gland disease) of workers. We found five species belonging to Formica s. str., and two of them showed changes in their relative abundance, which could not be explained by natural habitat differences. Nest mound volumes were 34% smaller in the polluted area, suggesting that smaller colonies can be maintained there. The heavy metal levels in F. aquilonia workers were higher in the polluted area for all metals, except Hg. The largest relative differences between the study areas (polluted/unpolluted) were found for As (4.1), Ni (2.4), Cu (2.1) and Pb (1.8). Morphological characters of workers were not related to the heavy metal levels. Our data showed that red wood ants can tolerate relatively high amounts of heavy metals and maintain reproducing colonies even in a heavily polluted area, but on the basis of smaller colony sizes, pollution stress may also cause trade-offs in reproduction. - Capsule: Five species of red wood ants vary in their sensitivity to heavy metal pollution but all of them had smaller colonies in a polluted area

  6. "Paterniplex", a highly discriminative decaplex STR multiplex tailored for investigating special problems in paternity testing.

    Science.gov (United States)

    Betz, Thomas; Immel, Uta-Dorothee; Kleiber, Manfred; Klintschar, Michael

    2007-11-01

    The goal of the study was to develop a STR multiplex ("Paterniplex") that is--as supplement to commercially available multiplex kits like the Identifiler kit (Applied Biosystems, Foster City, CA)--suitable for solving complex paternity cases such as deficiency cases or cases with mutations. The Paterniplex comprises the nine highly polymorphic STRs D8S1132, D7S1517, D10S2325, D12S391, Se33, D17S976, Penta E, Penta D and FGA in addition to Amelogenin as sex determination marker. The loci were selected because of their high degree of polymorphism (higher than that of the widely used TH01 marker). Only one locus, FGA, is shared with the Identifiler kit to avoid sample mix up. The study further gives details on the population genetics of the loci in a German Caucasian population (allelic distribution, Hardy-Weinberg Equilibrium and forensic efficiency markers such as the Discriminating Power) and three examples for cases that could not be solved using commercially available kits alone, but using the Paterniplex in addition to a commercial kit.

  7. Genetic Polymorphisms of Nine X-STR Loci in Four Population Groups from Inner Mongolia, China

    Institute of Scientific and Technical Information of China (English)

    Qiao-Fang Hou; Bin Yu; Sheng-Bin Li

    2007-01-01

    Nine short tandem repeat (STR) markers on the X chromosome (DXS101, DXS6789, DXS6799, DXS6804, DXS7132, DXS7133, DXS7423, DXS8378, and HPRTB) were analyzed in four population groups (Mongol, Ewenki, Oroqen, and Daur) from Inner Mongolia, China, in order to learn about the genetic diversity, forensic suitability, and possible genetic affinities of the populations. Frequency estimates, Hardy-Weinberg equilibrium, and other parameters of forensic interest were computed. The results revealed that the nine markers have a moderate degree of variability in the population groups. Most heterozygosity values for the nine loci range from 0.480 to 0.891, and there are evident differences of genetic variability among the populations. A UPGMA tree constructed on the basis of the generated data shows very low genetic distance betweent Mongol and Han (Xi'an) populations. Our results based on genetic distance analysis are consistent with the results of earlier studies based on linguistics and the immigration history and origin of these populations. The minisatellite loci on the X chromosome studied here are not only useful in showing significant genetic variation between the populations, but also are suitable for human identity testing among Inner Mongolian populations.

  8. Development of 19-plex Y STR system and polymorphism studies in Pakistani population

    Institute of Scientific and Technical Information of China (English)

    Faraz Malik; Faizan Raiz; Qurat-ul-ain; Muhammad Hassan Siddiqi; Allah Rakha; Zia ur Rehman; Zahoor Ahmed; Mahmood A. Kayani; M. Ansar; Obaid Ullah; Muhammad Shafeeq; Shahid Chohan; Yassir Abbas; Saqib Shazad,Ali Raza; Rahat Rehman

    2008-01-01

    For the development of 19-plex Y STR system and polymorphism studies in locl ethnic populations sixteen markers of non-recombining regions (NRY) of Y chromosome, which show high power of discrimination among individuals, were selected in this study. Blood samples (600) were e.ollected from the males of three most common castes of Pakistani population (Arnin, Awan and Rajput) with different parent lineages. Three markers (DYS385a/b, DYS389Ⅰ/Ⅱ and YCAⅡa/b) among 16 Y STRs are double-targeted regions of the Y chromosome and thus provide two polymorphie peaks for each respective primer set. These 16 Y-STRs were developed into Megaplex system for simultaneous amplification of all markers within the population. The overall power of discrimination observed in focused populations was 60.5%, 66.5% and 55% in Rajput, Awan and Arain casts respectively. This discrimination power will be helpful in haman identification for forensic casework studies including sexual assaults and paternity testing.

  9. Genetic variation of polymorphic NOS STR locus in ten Indian population groups.

    Science.gov (United States)

    Shazia, A; Nithya, P; Seshadri, M

    2009-02-01

    The genotyping of 313 random individuals belonging to ten different population groups from three different states of India was performed for polymorphic pentanucleotide repeat present in the 5'-flanking region of nitric oxide synthase gene (NOS2A) to study the effect of geographical and linguistic affiliations on the genetic affinities among these groups. Likelihood ratio tests showed that all the ten populations for this locus were in Hardy Weinberg equilibrium. Eleven different alleles ranging from 7 repeat to 17 repeats and 46 different genotypes were observed. The observed and the expected heterozygosity ranged from 0.72-0.94 and 0.84-0.89, respectively. The discriminating power of this locus is > or = 0.86 and the polymorphism information content of this locus in ten population groups ranged from 0.80 to 0.85. High PIC, PD and PE value of this STR showed this marker to be informative and can be used for DNA typing and population studies. The eight populations from Kerala showed a lower GST value of 0.016 compared to the GST of ten populations (G(ST) = 0.019), thereby showing that the populations from the same state showed higher genetic proximity probably due to linguistic and geographical proximity between them.

  10. Allelic structure and distribution of 103 STR loci in a Southern Tunisian population

    Indian Academy of Sciences (India)

    Abdellatif Maalej; Ahmed Rebai; Adnen Ayadi; Jomaa Jouida; Hafedh Makni; Hammadi Ayadi

    2004-04-01

    Genotypes of 103 short tandem repeat (STR) markers distributed at an average of 40 cM intervals throughout the genome were determined for 40 individuals from the village of BirEl Hfai (BEH). This village of approximately 31.000 individuals is localized in the south-west of Tunisia. The allele frequency distributions in BEH were compared with those obtained for individuals in the CEPH (Centre d’Etude du Polymorphisme Humain) data using a Kolmogorov–Smirnov two-sample test. Fourteen out of the 103 markers (13.2%) showed significant differences ($P\\lt 0.05$) in distribution between the two populations. Population heterogeneity in BEH was indicated by an excess of observed homozygosity deviations from Hardy–Weinberg equilibrium at 3 loci ($P\\lt 0.0005$). No evidence for genotypic disequilibrium was found for any of the marker pairs. This demonstrated that in spite of a high inbreeding level in the population, few markers showed evidence for a different pattern of allelic distribution compared to CEPH.

  11. Allele frequency data for 15 autosomal STR loci in eight Indonesian subpopulations.

    Science.gov (United States)

    Venables, Samantha J; Daniel, Runa; Sarre, Stephen D; Soedarsono, Nurtami; Sudoyo, Herawati; Suryadi, Helena; van Oorschot, Roland A H; Walsh, Simon J; Widodo, Putut T; McNevin, Dennis

    2016-01-01

    Evolutionary and cultural history can affect the genetic characteristics of a population and influences the frequency of different variants at a particular genetic marker (allele frequency). These characteristics directly influence the strength of forensic DNA evidence and make the availability of suitable allele frequency information for every discrete country or jurisdiction highly relevant. Population sub-structure within Indonesia has not been well characterised but should be expected given the complex geographical, linguistic and cultural architecture of the Indonesian population. Here we use forensic short tandem repeat (STR) markers to identify a number of distinct genetic subpopulations within Indonesia and calculate appropriate population sub-structure correction factors. This data represents the most comprehensive investigation of population sub-structure within Indonesia to date using these markers. The results demonstrate that significant sub-structure is present within the Indonesian population and must be accounted for using island specific allele frequencies and corresponding sub-structure correction factors in the calculation of forensic DNA match statistics.

  12. Further characterization of six miniSTR loci in the Han population from China

    Directory of Open Access Journals (Sweden)

    Zhen-jun JIA

    2012-02-01

    Full Text Available Objective  To describe the characteristics of two miniplex sets: NC01 (D10S1248, D14S1434 and D22S1045, which was recommended by EDNAP/ENFSI, and a new miniplex one (D2S2944, D18S872 and D19S591. Methods  DNA was extracted using the Chelex-100 extraction method. The products were genotyped by ABI PRISM® 310 Genetic Analyzer and the results were analyzed with GeneScan 3.7 and GenoTyper 3.7 software. Results  All loci meet Hardy-Weinberg equilibrium. The combined power of discrimination for the six loci in Chinese population was 0.9999 and the cumulative probability of exclusion was 0.9793. We also compared the sequencing data of NC01 with other different ethic groups. Conclusion  Two miniplex sets were constructed. These miniSTR makers have different characteristics in different ethic groups.

  13. Local population structure in Arabian Peninsula revealed by Y-STR diversity.

    Science.gov (United States)

    Alshamali, Farida; Pereira, Luísa; Budowle, Bruce; Poloni, Estella S; Currat, Mathias

    2009-01-01

    Genetic studies have been underway on Arabian Peninsula populations because of their pivotal geographic location for population migration and times of occurrence. To assist in better understanding population dynamics in this region, evidence is presented herein on local population structure in the Arabian Peninsula, based on Y-STR characterisation in four Arabian samples and its comparison in a broad geographical scale. Our results demonstrate that geography played an important role in shaping the genetic structure of the region around the Near-East. Populations are grouped regionally but none of these groups is significantly differentiated from others and all groups merge in the Near-East, in keeping with this important migration corridor for the human species. Focusing on the Arabian Peninsula, we show that Dubai and Oman share genetic affinities with other Near-Eastern populations, while Saudi Arabia and Yemen show a relative distinctive isolated background. Those two populations may have been kept relatively separated from migration routes, maybe due to their location in a desert area.

  14. Str\\"omgren uvby photometry of the peculiar globular cluster NGC 2419

    CERN Document Server

    Frank, Matthias J; Feltzing, Sofia; Kacharov, Nikolay; Wilkinson, Mark I; Irwin, Mike

    2015-01-01

    NGC 2419 is a peculiar Galactic globular cluster in terms of size/luminosity, and chemical abundance anomalies. Here, we present Str\\"omgren $uvby$ photometry of the cluster. Using the gravity- and metallicity-sensitive $c_1$ and $m_1$ indices, we identify a sample of likely cluster members extending well beyond the formal tidal radius with an estimated contamination by non-members of only 1%. We derive photometric [Fe/H] of red giants, and depending on which literature metallicity relation we use, find reasonable to excellent agreement with spectroscopic [Fe/H]. We demonstrate explicitly that the photometric errors are not Gaussian, and using a realistic model for the photometric uncertainties, find a formal internal [Fe/H] spread of $\\sigma=0.11^{+0.02}_{-0.01}$ dex. This is an upper limit to the cluster's true [Fe/H] spread and may partially/entirely reflect the limited precision of the photometric metallicity estimation and systematic effects. The lack of correlation between spectroscopic and photometric ...

  15. Population data of 17 Y-STR loci from Rio Grande do Sul state (South Brazil).

    Science.gov (United States)

    Schwengber, Solange P; Kommers, Trícia; Matte, Cecília H F; Raimann, Paulo E; Carvalho, Bianca A; Leite, Fabio P N; Medeiros, Marcelo A; Souza, Luis F; Castro, Cibele S; Chassot, Fernanda G C; Bonatto, Sandro L

    2009-12-01

    A sample of 255 Brazilian males from Rio Grande do Sul (RS), the Brazilian southernmost state, was typed for 17 Y-STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, YGATA_H4.1 and DYS385ab). A total of 247 haplotypes were identified, of which 239 were unique and eight were found in two individuals each. The haplotype diversity (99.98%) and discrimination capacity (96.86%) were calculated. Pairwise haplotype distances showed that the RS population is not significantly different from Brazil, Rio de Janeiro, and Argentina, is different from São Paulo, Italy, and North Portugal, and is very distant from Spain, the Amazon region, Germany, and South Amerindians. When the RS data was separated in the seven geopolitical regions, some pairs of regions were significantly different; however no region was different from the whole Brazilian sample. PMID:19948319

  16. STR genetic diversity in a Mediterranean population from the south of the Iberian Peninsula.

    Science.gov (United States)

    Gaibar, Maria; Esteban, Esther; Moral, Pedro; Gómez-Gallego, Félix; Santiago, Catalina; Bandrés, Fernando; Luna, Francisco; Fernández-Santander, Ana

    2010-04-01

    Sixteen Y-chromosomal short tandem repeats (STRs) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4.1) were typed in DNA samples from 52 unrelated men and 15 autosomal STRs (CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, vWA) were also studied for a group of 90 individuals (men and women) from the same population (Andalusians from La Alpujarra, South of Spain). The Alpujarrenian population represents an example of an isolated population with remarkable geographical, cultural and historical characteristics. High haplotype diversities were observed for the studied polymorphisms, 0.98 and 1 for YSTRs and autosomal STRs, respectively. Population comparisons for the autosomal STR allele distributions revealed remarkable levels of global homogeneity among samples geographically related. PMID:19961347

  17. Y chromosome STR allelic and haplotype diversity in a Rwanda population from East Central Africa.

    Science.gov (United States)

    Balamurugan, Kuppareddi; Duncan, George

    2012-03-01

    We have analyzed 17 Y-chromosomal STR loci in a population sample of 69 unrelated male individuals of the Rwanda-Hutu population from East Central Africa using an AmpFlSTR® Yfiler™ PCR amplification kit. A total of 62 unique haplotypes were identified among the 69 individuals studied. The haplotype diversity was found to be 0.9970 for this population. The gene diversity ranged from 0.1130 (DYS392) to 0.7722 (DYS385). Comparison of populations in this study with twenty-five other national and global populations using Principal Co-ordinate Analysis (PCA) and phylogenetic molecular analysis using a genetic distance matrix indicates a delineation of all the African populations from other unrelated populations. The results of population pair-wise Fst p values indicate statistically significant differentiation of the Rwandan population when compared with 25 other global populations including four African populations (p=0.0000). Analysis of Molecular Variance (AMOVA) of the Rwanda population with four other African populations indicated a 93% variance within populations and 7% variance among the five populations. A data base search of the 62 haplotypes yielded only one non-African haplotype match, suggesting these haplotypes are unique to the African continent. PMID:22285642

  18. Self-reported adherence supports patient preference for the single tablet regimen (STR in the current cART era

    Directory of Open Access Journals (Sweden)

    G Sterrantino

    2012-11-01

    Full Text Available Objective: To analyze self-reported adherence to antiretroviral regimens containing ritonavir-boosted protease inhibitors, non-nucleoside reverse transcriptase inhibitors (NNRTI, raltegravir, and maraviroc. Methods: Overall, 372 consecutive subjects attending a reference center for HIV treatment in Florence, Italy, were enrolled in the study, from December 2010 to January 2012 (mean age 48 years. A self-report questionnaire was filled in. Patients were defined as “non-adherent” if reporting one of the following criteria:<90% of pills taken in the last month, ≥1 missed dose in the last week, spontaneous treatment interruptions reported, or refill problems in the last 3 months. Gender, age, CD4, HIV-RNA, years of therapy, and type of antiretroviral regimen were analyzed with respect to adherence. Results: At the time of the questionnaire, 89.8% of patients had <50 copies/mL HIV-RNA and 14.2% were on their first combined antiretroviral therapy. 57% of patients were prescribed a regimen containing ritonavir boosted protease inhibitors (boosted PI, 41.7% NNRTI, 17.2% raltegravir, and 4.8% maraviroc; 49.5% of the subjects were on bis-in-die regimens, while 50.5% were on once-daily regimens, with 23.1% of these on the single tablet regimen (STR: tenofovir/emtricitabine/efavirenz. The non-adherence proportion was lower in NNRTI than in boosted-PI treatments (19.4% vs 30.2%, and even lower in STR patients (17.4%. In multivariable logistic regression, patients with the NNRTI regimen (OR: 0.56, 95% CI: 0.34–0.94 and the STR (OR: 0.45, 95% CI: 0.22–0.92 reported lower non-adherence. Efavirenz regimens were also associated with lower non-adherence (OR: 0.42, 95% CI: 0.21–0.83, while atazanavir/ritonavir regimens were associated with higher non-adherence. No other relation to specific antiretroviral drugs was found. A higher CD4 count, lower HIV-RNA, and older age were also found to be associated with lower non-adherence, while a longer time on

  19. Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples.

    Science.gov (United States)

    Thompson, Jonelle M; Ewing, Margaret M; Frank, William E; Pogemiller, Jill J; Nolde, Craig A; Koehler, D Jody; Shaffer, Alyssandra M; Rabbach, Dawn R; Fulmer, Patricia M; Sprecher, Cynthia J; Storts, Douglas R

    2013-02-01

    The PowerPlex® Y23 System combines the seventeen Y-STR loci in current commercially available Y-STR kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4) with six new highly discriminating Y-STR loci (DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643). These six new loci have higher gene diversities than most of the loci in other commercial Y-STR analysis kits, allowing for further distinction between unrelated male individuals. In addition, the inclusion of two rapidly mutating Y-STR loci may allow for the discrimination of related individuals. The PowerPlex® Y23 System is designed to amplify DNA from purified extracts as well as direct amplification from substrates used to collect database samples (e.g. swabs and storage cards). Validation of the PowerPlex® Y23 System includes all of the studies required by the FBI and SWGDAM. The results demonstrate that the PowerPlex® Y23 System is a robust and reliable amplification kit capable of overcoming high concentrations of commonly encountered inhibitors such as hematin, humic acid, and tannic acid. Full profiles are consistently detected with 62.5 pg of male DNA, even in the presence of excessive amounts of female DNA, establishing the PowerPlex(®) Y23 System as a sensitive method for Y-STR testing. Complete Y-STR profiles are detected from mixed samples with 62.5 pg of male DNA in a background of 400 ng of female DNA or 125 pg of male DNA mixed with 3000 ng of female DNA. PMID:23337322

  20. Genetic structure and diversity of three Colombian southwest afrodescendent populations using 8 STR's

    International Nuclear Information System (INIS)

    To estimate the diversity, structure and genetic flow in three Colombian southwest afrodescendent populations (Buenaventura, Mulalo y Tumaco), the alleles revealed by 8 autosomal STR's were analyzed in 78 no-related individuals, by the use of PCR and comparison with specific allelic ladders for every system resolved by polyacrylamide gel (8%). the results were compared with 2 Amerindian populations (Awa-Kuaikier and Coyaima) and 2 mixed Colombian populations (Valle del Cauca and Cauca). For the afrodescendent and Amerindian populations was found moderate diversity (h between 0.768±0.414 and 0.796±0.424), in contrast, the mixed population showed higher rates (>0.803), which is probably caused by mixing with Amerindians, that also can explain the high endogamy seen in mixed populations. The AMOVA exhibited moderate genetic structure between the afrodescendent populations (FST= 0.098; p<0.05), but higher between the three ethnical groups compared (FST=0.26723; p<0.05). The closer genetics distances are in favor of Tumaco and Buenaventura, supported for the migration rate found (34.298), which was the same inside of Amerindian and mixed populations. Maybe, because Mulalo is a closed isolated population, its differences in front others afrodescendent populations are explained. The neighbor-joining tree showed nearest relations among Amerindian and mixed populations, furthermore, the ancestral character for the afrodescendents. That sustains the idea of genetic flow maintained between the 3 ethnical groups, principally between Amerindian and mixed populations, supported because the genetic differences, migration rates and Amerindian matrilineality reported in the literature

  1. Population data of 21 non-CODIS STR loci in Han population of northern China.

    Science.gov (United States)

    Yuan, Li; Ge, Jianye; Lu, Di; Yang, Xue

    2012-07-01

    Allele frequencies and forensic statistics of 21 autosomal short tandem repeat loci (i.e., D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435 and D5S2500) were estimated in Han population from northern China (n = 220). Significant deviation from Hardy-Weinberg equilibrium was detected only for D22S1045. The observed heterozygosity, the expected heterozygosity, the discrimination power, the probability of paternity exclusion in trios, the probability of paternity exclusion in duos and the polymorphic information content ranged from 0.591 to 0.836, 0.594 to 0.830, 0.762 to 0.948, 0.341 to 0.659, 0.189 to 0.487 and 0.535 to 0.807, respectively. Triallelic patterns were observed at D19S433 and D10S1435. Mutations occurred at D22ATA63, D10S1248, D19S433 and D14S1434 loci with all single-step mutations. The expected mutation rates of these four loci are 0.0042 with 95% confidence interval [0.0001, 0.0232] in a total of 238 meioses. Our results show that these 21 non-CODIS STR loci are highly polymorphic and can be useful for human identification and kinship analysis in Northern Han population in China.

  2. Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China.

    Science.gov (United States)

    Zhang, Honghua; Yang, Shuping; Guo, Wei; Ren, Bo; Pu, Liwen; Ma, Teng; Xia, Mingying; Jin, Li; Li, Liming; Li, Shilin

    2016-09-01

    The six-dye GlobalFiler™ Express PCR amplification kit incorporates 21 commonly used autosomal short tandem repeat (STR) loci and three gender determination loci. In this study, we analyzed the GlobalFiler STR loci on 748 unrelated individuals from a Chinese Kazakh population of Xinjiang, China. No significant deviations from Hardy-Weinberg equilibrium and linkage disequilibrium were observed within and between 21 autosomal STR loci. SE33 showed the greatest power of discrimination in Kazakh population. The combined power of discrimination of Kazakh was 99.999999999999999999999996797 %. No significant differences of allele frequencies were observed between Kazakh and Uyghur at all 15 tested STR loci, as well as Mongolian. Significant differences were only observed between Kazakh and the other Chinese populations at TH01. Multiple STR loci showed significant differences between Kazakh and Arab, as well as South Portuguese. The multidimensional scaling plot (MDS) plot and neighbor-joining tree also showed Kazakh is genetically close to Uyghur. PMID:26846763

  3. The Wolbachia WO bacteriophage proteome in the Aedes albopictus C/wStr1 cell line: evidence for lytic activity?

    Science.gov (United States)

    Baldridge, Gerald D; Markowski, Todd W; Witthuhn, Bruce A; Higgins, LeeAnn; Baldridge, Abigail S; Fallon, Ann M

    2016-01-01

    Wolbachia pipientis (Rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. Modification of host population structure has potential applications for control of pest species, particularly if Wolbachia can be maintained, manipulated, and genetically engineered in vitro. Although Wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with distinct Wolbachia strains, and horizontal gene transfer between strains is potentially mediated by WO phages encoded within Wolbachia genomes. Proteomic analysis of a robust, persistent infection of a mosquito cell line with wStr from the planthopper, Laodelphax striatellus, revealed expression of a full array of WO phage genes, as well as nine of ten non-phage genes that occur between two distinct clusters of WOMelB genes in the genome of wMel, which infects Drosophila melanogaster. These non-phage genes encode potential host-adaptive proteins and are expressed in wStr at higher levels than phage structural proteins. A subset of seven of the non-phage genes is flanked by highly conserved non-coding sequences, including a putative promoter element, that are not present in a syntenically arranged array of homologs in plasmids from three tick-associated Rickettsia spp. These studies expand our understanding of wStr in a host cell line derived from the mosquito, Aedes albopictus, and provide a basis for investigating conditions that favor the lytic phase of the WO phage life cycle and recovery of infectious phage particles. PMID:26427709

  4. STR analysis of human DNA from maggots fed on decomposing bodies: Assessment of the time period for successful analysis

    Directory of Open Access Journals (Sweden)

    Daniel Gachuiri Njau

    2016-09-01

    Full Text Available Frequently, forensic entomology is applied in the use of insect maggots for the identification of specimens or remains of humans. Maggot crop analysis could be valuable in criminal investigations when maggots are found at a crime scene and a corpse is absent. Human short tandem repeat (STR has previously been used to support the association of maggots to a specific corpse but not in the period at which the body has been decomposing. The aim of this research was to assess the time period for successful STR analyses of human DNA from third instar maggots (Protophormia terraenovae obtained from decomposing human corpses as well as to investigate the human DNA turnover and degradation in the maggot crop after they are removed from food and/or are fed on a beef (a new/different food source. Results showed that the amount of human DNA recovered from maggots decreased with time in all cases. For maggots fed on beef, the human DNA could only be recovered up to day two and up to day four for the starved maggots. STR analyses of human DNA from maggots’ crop content using 16 loci generated profiles that matched those of reference samples although some of the alleles were not amplifiable therefore generating partial profiles for the samples starved for 4 days and those fed on beef. This may be due to nuclease activity present in the gut of larvae that may have caused degradation of DNA and consequently reduction in DNA yield. It was possible to identify the decomposing body using STRs as markers.

  5. Fast nuclear staining of head hair roots as a screening method for successful STR analysis in forensics

    OpenAIRE

    Lepez, Trees; Vandewoestyne, Mado; Van Hoofstat, David; Deforce, Dieter

    2014-01-01

    The success rate of STR profiling of hairs found at a crime scene is quite low and negative results of hair analysis are frequently reported. To increase the success rate of DNA analysis of hairs in forensics, nuclei in hair roots can be counted after staining the hair root with DAPI. Two staining methods were tested: a longer method with two 1 h incubations in respectively a DAPI-and a wash-solution, and a fast, direct staining of the hair root on microscope slides. The two staining meth...

  6. Self-reported adherence supports patient preference for the single tablet regimen (STR in the current cART era

    Directory of Open Access Journals (Sweden)

    Sterrantino G

    2012-06-01

    Full Text Available Gaetana Sterrantino,1 Lucia Santoro,1 Dario Bartolozzi1,1 Michele Trotta,1 Mauro Zaccarelli21SOD Malattie Infettive, Azienda Ospedaliera Universitaria Careggi, Firenze, 2Istituto Nazionale per le Malattie Infettive "Lazzaro Spallanzani," Roma, ItalyObjective: To analyze self-reported adherence to antiretroviral regimens containing ritonavir-boosted protease inhibitors, nonnucleoside reverse transcriptase inhibitors (NNRTI, raltegravir, and maraviroc.Methods: Overall, 372 consecutive subjects attending a reference center for HIV treatment in Florence, Italy, were enrolled in the study, from December 2010 to January 2012 (mean age 48 years. A self-report questionnaire was filled in. Patients were defined as "nonadherent" if reporting one of the following criteria: <90% of pills taken in the last month, ≥1 missed dose in the last week, spontaneous treatment interruptions reported, or refill problems in the last 3 months. Gender, age, CD4, HIV-RNA, years of therapy, and type of antiretroviral regimen were analyzed with respect to adherence.Results: At the time of the questionnaire, 89.8% of patients had <50 copies/mL HIV-RNA and 14.2% were on their first combined antiretroviral therapy. 57% of patients were prescribed a regimen containing ritonavir boosted protease inhibitors (boosted PI, 41.7% NNRTI, 17.2% raltegravir, and 4.8% maraviroc; 49.5% of the subjects were on bis-in-die regimens, while 50.5% were on OD regimens, with 23.1% of these on the single tablet regimen (STR: tenofovir/emtricitabine/efavirenz. The nonadherence proportion was lower in NNRTI than in boosted-PI treatments (19.4% vs 30.2%, and even lower in STR patients (17.4%. In multivariable logistic regression, patients with the NNRTI regimen (OR: 0.56, 95% CI: 0.34–0.94 and the STR (OR: 0.45, 95% CI: 0.22–0.92 reported lower nonadherence. Efavirenz regimens were also associated with lower nonadherence (OR: 0.42, 95% CI: 0.21–0.83, while atazanavir/ritonavir regimens were

  7. Population genetics for 17 Y-STR loci(AmpFISTR®Y-filerTM) in Luzhou Han ethnic group.

    Science.gov (United States)

    Bing, Long; Liang, Weibo; Pi, Jianhua; Zhang, Deming; Yong, Dai; Luo, Haibo; Zhang, Lushun; Lin, Zhang

    2013-02-01

    In this study, 17 Y-STR loci(AmpFISTR(®)Y-filerTM)-DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA H4, DYS437, DYS438, DYS448 were analyzed in 424 unrelated males from Luzhou Han ethnic group, Southwest China. 365 haplotypes were observed. The discrimination capacity was 0.8608 and the haplotype diversity was 0.9992. PMID:23298877

  8. Strömungssimulation und experimentelle Untersuchung für innovative Verflüssiger auf Basis neuartiger Rohre

    OpenAIRE

    Schaake, Katrin; Manzke, Sebastian

    2009-01-01

    In dieser Arbeit werden neuartige Flachrohre für die Verwendung als Rückwandverflüssiger in der Haushaltskältetechnik mit numerischen und dynamischen Simulationen sowie Experimenten untersucht. Dabei kommen unterschiedliche überströmte Längen sowie der Einfluss horizontaler Abstände auf den Wärmeübergang durch freie Konvektion zur Betrachtung. Realisiert wird die numerische Strömungssimulation mit der Software Fluent 3.6.26, wobei das RNG-k-epsilon- als Turbulenzmodell und diskrete Ordinaten ...

  9. STR-PCR定量检测嵌合体供者嵌合率准确性探讨%Evaluation of the accuracy of STR-PCR to quantitate chimera

    Institute of Scientific and Technical Information of China (English)

    胡彬; 阎慧芝; 刘晓华; 李长缨; 冯智慧; 温振科; 焦淑贤

    2009-01-01

    目的 探讨用荧光标记复合扩增短串联重复序列(STR-PCR)结合序列分析仪毛细管电泳法检测嵌合体供者细胞嵌合率的准确性.方法 采集健康献血者外周血按白细胞比例两两混合制备不同供受者比例的体外嵌合体模型,对15个SIR位点进行荧光标记复合扩增,扩增产物进行序列分析仪毛细管电泳,计算嵌合率.结果 实验测得嵌合率与扩增前样本嵌合率呈显著直线相关,r=0.999 7,最小检出供者细胞嵌合率0.05),0.05) ; however, statistical difference between them was found in mixtures with chimerism rate <5%(P<0.05). Conclusion The chimerism of patients after allogeneic stem cell transplantation can be accurately determined by STR-PCR when the chimerism rate is ≥ 5%.

  10. Retrospective genetic study of germinative mutations in Str loci of individuals potentially exposed to ionizing radiation

    International Nuclear Information System (INIS)

    21S11 loeus and 03 mutations on FGA loeus, comprising a total of 11 mutations and a mutation rate of 0.008. In such context, we did not find significant differences (p= 0.15), indicating a possible exposure effect on the mutation rates of the STR loci, in the group accidentally exposed to Cesium-137. (author)

  11. Concordance study between the ParaDNA® Intelligence Test, a rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR® SGM Plus®).

    Science.gov (United States)

    Ball, G; Dawnay, N; Stafford-Allen, B; Panasiuk, M; Rendell, P; Blackman, S; Duxbury, N; Wells, S

    2015-05-01

    The ParaDNA® Intelligence Test enables STR profiling directly from human biological samples and evidence items collected from crime scene in 75min. Designed for non-expert use this system allows DNA information to be available to investigators before it would typically be available from a laboratory. The ParaDNA Intelligence Test system amplifies D3S1358, D8S119, D16S539, D18S1358 and TH01 STR loci and the gender typing locus amelogenin and detects the alleles present with HyBeacon® probes. Individual DNA samples from 381 UK Caucasian individuals were analysed using AmpFlSTR® SGM Plus® and the ParaDNA Intelligence Test with the derived STR profiles compared. Here we describe the high level of concordance demonstrated between the two systems and discuss this with reference to allele frequencies and the discriminatory power offered by the ParaDNA Intelligence Test. PMID:25528026

  12. Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China*

    Science.gov (United States)

    Wang, Hong-dan; Shen, Chun-mei; Liu, Wen-juan; Zhang, Yu-dang; Yang, Guang; Yan, Jiang-wei; Qin, Hai-xia; Zhu, Bo-feng

    2013-01-01

    We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus, which are not included in the combined DNA index system (CODIS), in a Russian ethnic minority group from the Inner Mongolia Autonomous Region, China. A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system. Using capillary electrophoresis, the PCR products of the 21 STR loci were separated and genotyped. A total of 161 alleles were observed in the Russian ethnic minority group, and corresponding allelic frequencies ranged from 0.0044 to 0.5965. The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population’s genetic background, for individual identification, and for paternity testing in forensic practice. PMID:23733431

  13. Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China.

    Science.gov (United States)

    Wang, Hong-dan; Shen, Chun-mei; Liu, Wen-juan; Zhang, Yu-dang; Yang, Guang; Yan, Jiang-wei; Qin, Hai-xia; Zhu, Bo-feng

    2013-06-01

    We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus, which are not included in the combined DNA index system (CODIS), in a Russian ethnic minority group from the Inner Mongolia Autonomous Region, China. A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system. Using capillary electrophoresis, the PCR products of the 21 STR loci were separated and genotyped. A total of 161 alleles were observed in the Russian ethnic minority group, and corresponding allelic frequencies ranged from 0.0044 to 0.5965. The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background, for individual identification, and for paternity testing in forensic practice.

  14. Optimizing eSTR Algorithm for Solving Constraint Satisfaction Problems%求解约束可满足问题的eSTR算法优化

    Institute of Scientific and Technical Information of China (English)

    王瑞伟; 李占山; 李宏博

    2016-01-01

    表约束方法是1种外延式知识表示方法,每个约束通过元组集直接枚举出其在1个变量集上允许或禁止的所有元组,直观易于理解,在约束程序中得到了深入的研究,这是因为表约束出现在如设计、数据库、配置以及偏好建模等许多现实世界的应用中.但随着约束的增多及其元组集增大,占有的空间与相容性检测效率成了关键问题.eSTR是1个将STR扩展到高阶相容的算法,通过深入分析eSTR算法后发现有2种优化方式:PWsup数据结构和极小约束范围,同时证明了在极小约束范围上,约束网络仍然能够维持PWC+GAC ,其中极小约束范围可以通过删除约束元组集中相应的列来降低eSTR2算法的空间消耗,而 PWsup数据结构能够通过避免不必要的PW 支持检测来减少eSTR2的CPU 运行时间,实验结果表明:2种优化方式和eSTR2相结合后能够同时减少算法的空间消耗和CPU 运行时间,在许多类实例上明显优于eS T R2和eS T R2w .%Table constraints ,i .e .,constraints given in extension by listing all allowed (or forbidden) tuples ,are very straight forward and easy to understand ,which are intensively studied in constraint programming (CP) .Because such constraints are presented in many real world applications from areas such as design ,databases ,configuration and preferences modeling . However ,With the grow th of number of constraints and number of tuples , the space cost for table constraints and time cost of consistency checking have become key topics .eSTR is an algorithm which extends simple tabular reduction (STR) to higher‐order consistency .After in‐depth analysis of eSTR algorithm ,this paper proposes two kinds of optimized methods for eSTR :PWsup data structure and minimal constraint scope ,and then we prove that the constraint network enforce Pair‐Wise Consistency and Generalized Arc‐Consistency (PWC + GAC ) with minimal constraint

  15. Genetic Variation of 25 Y-Chromosomal and 15 Autosomal STR Loci in the Han Chinese Population of Liaoning Province, Northeast China

    Science.gov (United States)

    Yao, Jun; Wang, Bao-jie

    2016-01-01

    In the present study, we investigated the genetic characteristics of 25 Y-chromosomal and 15 autosomal short tandem repeat (STR) loci in 305 unrelated Han Chinese male individuals from Liaoning Province using AmpFISTR® Yfiler® Plus and IdentifilerTM PCR amplification kits. Population comparison was performed between Liaoning Han population and different ethnic groups to better understand the genetic background of the Liaoning Han population. For Y-STR loci, the overall haplotype diversity was 0.9997 and the discrimination capacity was 0.9607. Gene diversity values ranged from 0.4525 (DYS391) to 0.9617 (DYS385). Rst and two multi-dimensional scaling plots showed that minor differences were observed when the Liaoning Han population was compared to the Jilin Han Chinese, Beijing Han Chinese, Liaoning Manchu, Liaoning Mongolian, Liaoning Xibe, Shandong Han Chinese, Jiangsu Han Chinese, Anhui Han Chinese, Guizhou Han Chinese and Liaoning Hui populations; by contrast, major differences were observed when the Shanxi Han Chinese, Yunnan Bai, Jiangxi Han Chinese, Guangdong Han Chinese, Liaoning Korean, Hunan Tujia, Guangxi Zhuang, Gansu Tibetan, Xishuangbanna Dai, South Korean, Japanese and Hunan Miao populations. For autosomal STR loci, DP ranged from 0.9621 (D2S1338) to 0.8177 (TPOX), with PE distributing from 0.7521 (D18S51) to 0.2988 (TH01). A population comparison was performed and no statistically significant differences were detected at any STR loci between Liaoning Han, China Dong, and Shaanxi Han populations. The results showed that the 25 Y-STR and 15 autosomal STR loci in the Liaoning Han population were valuable for forensic applications and human genetics, and Liaoning Han was an independent endogenous ethnicity with a unique subpopulation structure. PMID:27483472

  16. Diversity of five novel Y-STR loci and their application in studies of north Chinese populations

    Indian Academy of Sciences (India)

    Zhaoyang Xu; Haiming Sun; Yang Yu; Yan Jin; Xaingning Meng; Donglin Sun; Jing Bai; Feng Chen; Songbin Fu

    2010-04-01

    Y-chromosomal short tandem repeats (Y-STRs) show sufficient variability among individuals in a population and high degree of geographical differentiation, so their polymorphic character makes them especially suited for population genetic studies. In this study, five novel Y-STR loci were analysed in 174 samples from five minority populations residing in north China (Daur, Kazak, Xibe, Uighur and Kirgiz) to determine the diversity of these loci in north China and to evaluate their usefulness in population study. Ninety-seven haplotypes were constructed, with 30 in Daur, 24 in Kazak, 28 in Uighur, 27 in Xibe and 16 in Kirgiz. Sixty-six (68.04%) of them were unique. The $R_{\\text{ST}}$ showed that there was no significant difference in Daur and Xibe ($R_{\\text{ST}} = 0.02231$, $P \\gt 0.05$), while among the Kazak, Uighur and Kirgiz, who reside in northwest China, there were significant differences. These results showed that these five Y-STR loci were polymorphic in the five populations. The results of AMOVA showed that majority of the differences were found within populations. By $R_{\\text{ST}}$, the relationships of the five populations were accordance with the historical records: Xibe migrated to Xinjiang during the Qing Dynasty, and Kazak, Uighur and Kirgiz have different ancestors.

  17. Analysis of 16 Y STR loci in the Finnish population reveals a local reduction in the diversity of male lineages.

    Science.gov (United States)

    Hedman, M; Pimenoff, V; Lukka, M; Sistonen, P; Sajantila, A

    2004-05-28

    We analysed samples of 400 Finnish males using nine Y-chromosomal short tandem repeat (STR) loci (minimal haplotype); for 200 of these subjects an additional seven Y-chromosomal STR loci were used. The geographical distribution of the observed haplotypes was determined from 200 individuals of known paternal origin within Finland. The observed number of alleles varied from 2 to 13 alleles per locus. A total of 146 minimal haplotypes were identified in our population sample. Interestingly, 90 (22.5%) individuals shared an identical haplotype. This haplotype was extremely frequent in the northern and eastern subpopulations of Savo, Pohjanmaa and Karjala (53, 42 and 37%, respectively). With the seven additional loci analysed in the sample of 200 individuals, 120 haplotypes were identified, and individuals sharing the most common haplotype decreased to 13.0%. However, in comparison to other European populations, the Finnish population showed decreased genetic diversity (GD) when the number of different minimal haplotypes in the population was divided by the sample size (36.5% in Finns versus 83.7% on average). Our results strongly support the earlier hypothesis of individual isolated Y-chromosomal lineages and population substructuring in Finland. For paternity testing, power of exclusion was 92% using minimal haplotype data, but including the seven additional loci this value increased to 97%.

  18. Development of the 19 X-STR loci multiplex system and genetic analysis of a Zhejiang Han population in China.

    Science.gov (United States)

    Yang, XingYi; Wu, WeiWei; Chen, LinLi; Liu, ChangHui; Zhang, XiaoFang; Chen, Ling; Feng, XingLin; Wang, HuiJun; Liu, Chao

    2016-08-01

    The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing. PMID:27184937

  19. MiniSTR multiplex systems based on non-CODIS loci for analysis of degraded DNA samples.

    Science.gov (United States)

    Asamura, H; Fujimori, S; Ota, M; Fukushima, H

    2007-11-15

    We describe two short amplicon autosomal short tandem repeat (miniSTR) quadruplex systems for eight loci D1S1171, D2S1242, D3S1545, D4S2366, D12S391, D16S3253, D20S161, and D21S1437, unlinked from the combined DNA index system (non-CODIS) loci, using newly designed primer sets. The results of an assay of 411 Japanese individuals showed that polymerase chain reaction (PCR) products within the eight loci were less than 150bp in size, without the seven additional bases for adenylation. The frequency distributions in the loci showed no deviations from Hardy-Weinberg equilibrium expectations. The accumulated power of discrimination and power of exclusion for the eight loci were 0.9999999991 and 0.998, respectively. For assay of highly degraded DNA, including artificially degraded samples and the degraded forensic casework samples assessed with the present miniSTR quadruplex systems, the systems proved quite effective in analyzing degraded DNA.

  20. Genetic polymorphism of 23 Y-STR loci in the Zhuang minority population in Guangxi of China.

    Science.gov (United States)

    Luo, Haibo; Song, Feng; Zhang, Lushun; Hou, Yiping

    2015-07-01

    In the present study, 23 Y-STR loci (DYS576, DYS389I, DYS389 II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS393, DYS458 DYS456, DYS643, YGATAH4, and DYS385ab) were investigated in 266 unrelated, healthy autochthonous individuals from the Zhuang minority population residing in the Guangxi Zhuang Autonomous Region, China. One hundred and eighty-nine alleles and 245 haplotypes were found in the Zhuang group. Two hundred and twenty-four haplotypes among them were unique, and the remaining 21 haplotypes were found in two individuals. Discrimination capacity was 0.9211. Haplotype diversity was 0.9993 and gene diversity ranged from 0.4173 (DYS437) to 0.9678 (DYS385ab). Populations' differentia was calculated and compared with Tibetan, Bai, Dai, Minnan Han, Beijing Han, Chengdu Han, Xuanwei Han, and Southern Han ethnic groups in China, the Singapore Han population, and the Kinh group from Ho Chi Minh City, Vietnam, in the same 23 Y-STR loci. Our results showed that these 23 Y-STRs are highly genetically polymorphic in the Zhuang group and can also enrich Chinese ethnic genetic information. PMID:25877764

  1. SkydancerPlex: A novel STR multiplex validated for forensic use in the hen harrier (Circus cyaneus).

    Science.gov (United States)

    van Hoppe, Moniek J C; Dy, Mary A V; van den Einden, Marion; Iyengar, Arati

    2016-05-01

    The hen harrier (Circus cyaneus) is a bird of prey which is heavily persecuted in the UK because it preys on the game bird red grouse (Lagopus lagopus scoticus). To help investigations into illegal killings of hen harrier, a STR multiplex kit containing eight short tandem repeat (STR) markers and a chromohelicase DNA binding protein 1 (CHD 1) sexing marker was developed. The multiplex kit was tested for species specificity, sensitivity, robustness, precision, accuracy and stability. Full profiles were obtained with as little as 0.25 ng of template DNA. Concurrent development of an allelic ladder to ensure reliable and accurate allele designation across laboratories makes the SkydancerPlex the first forensic DNA profiling system in a species of wildlife to be fully validated according to SWGDAM and ISFG recommendations. An average profile frequency of 3.67 × 10(-8), a PID estimate of 5.3 × 10(-9) and a PID-SIB estimate of 9.7 × 10(-4) make the SkydancerPlex an extremely powerful kit for individualisation. PMID:26881329

  2. Interpreting the low-frequency radio spectra of starburst galaxies: a pudding of Strömgren spheres

    Science.gov (United States)

    Lacki, Brian C.

    2013-06-01

    The low-frequency radio emission of starburst galaxies is informative, but it can be absorbed in several ways. Most importantly, starburst galaxies are home to many H II regions, whose free-free absorption blocks low-frequency radio waves. These H II regions are discrete objects, but most multiwavelength models of starbursts assume a uniform medium of ionized gas, if they include the absorption at all. I calculate the effective absorption coefficient of H II regions in starbursts, which is ultimately a cross-section times the density of H II regions. The cross-sections are calculated by assuming that H II regions are Strömgren spheres. The coefficient asymptotes to a constant value at low frequencies, because H II regions partially cover the starburst and are buried part way into the starburst's synchrotron-emitting material. Considering Strömgren spheres around either OB stars or Super Star Clusters, I demonstrate the method by fitting to the low-frequency radio spectrum of M82. I discuss implications of the results for synchrotron spectrum shape, H II region pressure and free-free emission as a star formation rate indicator. However, these results are preliminary and could be affected by systematics. I argue that there is no volume-filling warm ionized medium in starbursts and that H II regions may be the most important absorption process down to ˜10 MHz. Future data at low and high radio frequency will improve our knowledge of the ionized gas.

  3. PCR typing of DNA fragments of the two short tandem repeat (STR) systems upstream of the human myelin basic protein (MBP) gene in Danes and Greenland Eskimos

    DEFF Research Database (Denmark)

    Nellemann, L J; Frederiksen, J; Morling, N

    1996-01-01

    DNA from the double short tandem repeat (STR) system MBP (locus 18q23-pter) was amplified by the polymerase chain reaction (PCR) and the two polymorphic repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments of the two MBP STR systems MBP......-A and MBP-B were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. DNA samples from 112 unrelated Danes, 140 unrelated Greenland Eskimos, and 88 Danish mother/child pairs were analyzed. The distributions of MBP phenotypes were in Hardy-Weinberg equilibrium in both...

  4. Forensic Application of Expressmarker 22 STR Loci Direct PCR Amplification Kit%Expressmarker 22 STR荧光检测试剂盒的法医学应用

    Institute of Scientific and Technical Information of China (English)

    邹凯南; 曹禹; 夏子芳; 郑卫国; 周怀谷

    2012-01-01

    Objective To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit. Methods One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler? kit, Identifiler(R) kit and PowerPlex(R) 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit. Results 97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler? kit, Identifiler(R) kit and PowerPlex(R) 16 kit at the same STR loci. Conclusion Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results and be applied to forensic DNA genotyping.%目的 验证Expressmarker 22 STR荧光检测试剂盒的法医学应用价值.方法 取FTA卡、滤纸上保存的建库血样和各类涉案生物性检材1948份,用Expressmarker 22 STR荧光检测试剂盒进行STR分型检测,同时采用SinofilerTM、Identifiler(R)、PowerPlex(R) 16试剂盒进行平行试验.对4个试剂盒相同基因座的分型进行比对,以确认Expressmarker 22 STR荧光检测试剂盒的灵敏度和准确性.结果 Expressmarker 22 STR荧光检测试剂盒的检测成功率为97.79%,同一样本在相同基因座与SinofilerTM、Identifiler(R)、PowerPlex(R) 16试剂盒的STR分型结果相同.结论 利用Expressmarker 22 STR荧光检测试剂盒进行STR分型,信息量大、结果准确可靠.可应用于法庭科学.

  5. Noncell- and cell-autonomous G-protein-signaling converges with Ca2+/mitogen-activated protein kinase signaling to regulate str-2 receptor gene expression in Caenorhabditis elegans.

    NARCIS (Netherlands)

    H. Lans (Hannes); G. Jansen (Gert)

    2006-01-01

    textabstractIn the sensory system of C. elegans, the candidate odorant receptor gene str-2 is strongly expressed in one of the two AWC neurons and weakly in both ASI neurons. Asymmetric AWC expression results from suppression of str-2 expression by a Ca2+/MAPK signaling pathway in one of the AWC neu

  6. El subgénero Trigona S. Str. Jurine 1808 (Hymenoptera: Apidae: Meliponinae en Colombia

    Directory of Open Access Journals (Sweden)

    Hernández Martínez Édgar Javier

    2004-12-01

    Full Text Available Para Colombia se registran 28 de los 29 taxones descritos para el subgénero Trigona s. str. T. (T. albipennis Almeida, 1992; T. (T. amalthea Olivier, 1789; T. (T. hyalinata var. amazonensis
    Ducke, 1916; T. (T. hyalinata var. branneri Cockerell, 1912; T. (T. chanchamayoënsis Schwarz, 1948; T. (T. cilipes Fabricius, 1804; T. (T. corvina Cockerell, 1913; T. (T. crassipes Fabricius, 1793; T. (T. dallatorreana Friese, 1900; T. (T. dimidiata var. venezuelana Schwarz, 1948; T. (T. dimidiata var. dimidiata F. Smith, 1854; T. (T. ferricauda Cockerell, 1917; T. (T. fulviventris var. fulviventris Guerin, 1835; T. (T. fulviventris var. guianae Cockerell, 1910; T. (T. fuscipennis Friese,
    1900; T. (T. hyalinata var. hyalinata Lepeletier, 1836; T. (T. hypogea Silvestri, 1902; T. (T. mazucatoi Almeida, 1992; T. (T. necrophaga Camargo y Roubik, 1991; T. (T. nigerrima Cresson, 1878; T. (T. pallens Latreille, 1804; T. (T. permodica Almeida, 1992; T. (T. recursa F. Smith, 1863; T. (T. setentrionalis Almeida, 1992; T. (T. silvestriana Vachal, 1908; T. (T. spinipes Fabricius, 1793; T. (T. truculenta Almeida, 1984 y T. (T. williana Friese, 1900. Se reportan por primera vez los machos
    de T. (T. hyalinata var. amazonensis y T. (T. ferricauda los cuales se describirán en un trabajo posterior. Se encontró a T. (T. permodica y T. (T. necrophaga como registros nuevos para el país. El subgénero presenta una amplia distribución geográfica y altitudinal, encontrándose desde los 0 hasta los 2.600 msnm, todas las especies se encuentran en los bosques tropicales de Colombia. Existe una alta concentración de taxones (25 taxones; 89% de los taxones del país en la región del piedemonte llanero entre los departamentos de Cundinamarca, Boyacá y Meta. Se presenta una clave taxonómica ilustrada para separar obreras de las especies neotropicales; con diagnosis, comentarios sobre distribución, taxonomía y hábitos de nidificación de cada una de ellas. Tambi

  7. Construction of two fluorescence-labeled non-combined DNA index system miniSTR multiplex systems to analyze degraded DNA samples in the Chinese Han Population.

    Science.gov (United States)

    Bai, Xue; Li, Shujin; Cong, Bin; Li, Xia; Guo, Xia; He, Lujun; Ye, Jian; Pei, Li

    2010-09-01

    MiniSTR loci have been demonstrated to be an effective approach in recovering genetic information from degraded specimens, because of the reduced PCR amplicon sizes which improved the PCR efficiency. Eight non-combined DNA index system miniSTR loci suitable for the Chinese Han Population were analyzed in 300 unrelated Chinese Han individuals using two novel five fluorescence-labeled miniSTR multiplex systems(multiplex I: D10S1248, D2S441, D1S1677 and D9S2157; multiplex II: D9S1122, D10S1435, D12ATA63, D2S1776 and Amelogenin). The allele frequency distribution and forensic parameters in the Chinese Han Population were reported in this article. The Exact Test demonstrated that all loci surveyed here were found to be no deviation from Hardy-Weinberg equilibrium. The accumulated power of discrimination and power of exclusion for the eight loci were 0.999999992 and 0.98, respectively. The highly degraded DNA from artificially degraded samples and the degraded forensic case work samples was assessed with the two miniSTR multiplex systems, and the results showed that the systems were quite effective.

  8. Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y-chromosomal Short Tandem Repeat Analysis for Archeological Human Bones.

    Science.gov (United States)

    Oh, Chang Seok; Lee, Soong Deok; Shin, Kyoung-Jin; Shin, Dong Hoon

    2016-03-01

    The AmpFℓSTR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y-STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom-designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y-STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom-designed primers). In this study, the authors established that the custom-designed primers offer a markedly improved success rate for obtainment of Y-STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay. PMID:26375610

  9. Draft genome sequence of Lactococcus garvieae str. PAQ102015-99, an outbreak strain isolated from a commercial trout farm in the Northwestern United States.

    Science.gov (United States)

    We announce the draft genome assembly of Lactococcus garvieae str. PAQ102015-99, a recently isolated strain from an outbreak of lactococcosis at a commercial trout farm in the Northwestern US. The draft genome comprises 14 contigs totaling 2,068,357 bp with an N50 of 496,618 bp and average G+C conte...

  10. Study on STR Genotyping of Cell Free DNA in Plasma%血浆游离DNA的STR分型检测研究

    Institute of Scientific and Technical Information of China (English)

    陈阳; 胡利平; 马波; 马立宇; 聂胜洁

    2014-01-01

    目的:探讨利用血浆中游离DNA进行短串联重复序列(short tandem repeat,STR)分型检测,解决法医学个体识别和亲权鉴定问题的可行性。方法采集36例无关健康个体EDTA-Na2抗凝血样,分离血浆,采用经典酚-氯仿法分别处理血浆和血细胞,对提取的DNA进行15个STR基因座常规PCR扩增和荧光标记复合扩增,采用聚丙烯酰胺凝胶电泳和毛细管电泳检测2种STR分型方法进行检测。结果常规PCR扩增银染检测和荧光标记复合扩增毛细管电泳检测两种STR分型方法的结果表明,同一个体的血浆游离DNA和血细胞DNA STR分型一致,且分型效果接近。结论血浆游离DNA可作为一种有效的生物学样本进行STR分型检测,应用于法医个体识别和亲权鉴定。%Objective The purpose of this study was to investigate the feasibility of short tandem repeat(STR) genotyping of cell free DNA in plasma for individual identification and paternity testing. Methods EDTA-Na2 DNA anti-coagulant blood samples were collected from 36 unrelated healthy volunteers,and both DNA in leukocytes and cell free DNA in plasma were extracted respectively using phenol-chloroform method. Target DNA in blood cells and plasma were amplified using regular STR typing and fluorescent multiplex STR assay separately,accordingly,the PCR products were analyzed by polyacrylamide gel electrophoresis and capillary electrophoresis. Results Using either normal PCR-STR or fluorescent multiplex STR assay,the consistent STR genotyping results were detected with similar efficiency for cell DNA and plasma DNA samples from the same individual. Conclusion Cell free DNA in plasma samples can be used as useful biological samples for STR genotyping,which can be applied to individual identification and paternity testing in forensic practice.

  11. NIKE GOLF STR-8 FIT球杆发表会暨员工专业培训会

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    2009年3月13日,NIKE GOLF 2009新品STR-8FIT球杆发表会在深圳沙河球会会所隆重举办。NIKE大中华区总经理黄鸿达、NIKE GOLF中港澳总代理:深圳名将贸易有限公司(MDC)执行董事游国琛以及NIKE GOLF中港澳各经销商及店铺员工亲临现场,共同领略NIKE GOLF可调节式八种杆头位置的一号发球木杆。

  12. Genetic differences among North African Berber and Arab-speaking populations revealed by Y-STR diversity.

    Science.gov (United States)

    Gaibar, Maria; Esteban, Esther; Harich, Nourdin; Kandil, Mostafa; Fernández-Santander, Ana

    2011-03-01

    Y-chromosome STR polymorphisms are inherited in a haploid state which makes them a powerful tool for easy tracing of paternal lineage and for use in human population evolutionary studies. North-African Y chromosomal diversity has traditionally been studied in order to find genetic and geographic associations as well as to test how natural and cultural barriers have affected the degree of genetic flow not only within North Africa but also in a wider Mediterranean context. The degree of Berber/Arab genetic differentiation in the Moroccan population has been tested for a complete set of forensic markers as sixteen Y-chromosomal short tandem repeats (STRs) (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and GATA H4.1). The results suggest considerable population heterogeneity in North Africa. PMID:20854231

  13. Haplotype frequencies for 17 Y-STR loci (AmpFlSTRY-filer) in a Moroccan population sample.

    Science.gov (United States)

    Aboukhalid, Rachid; Bouabdellah, Mehdi; Abbassi, Meriame; Bentayebi, Kaoutar; Elmzibri, Mohammed; Squalli, Driss; Amzazi, Saaïd

    2010-04-01

    A sample of 267 unrelated Moroccan males from different ethnic groups (Arabs, Berbers and Sahrawi), was typed for 17 Y-STR loci (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y GATA H4). Discrimination capacity (96.3%) and haplotype diversity (99.91%) were calculated. A total of 257 haplotypes were identified, of which 237 were unique and 10 were found in two individuals each. DYS385 showed the highest diversity (0.887) followed by DYS458 (0.820) as a single locus marker. PMID:20215021

  14. Assembly of a large Y-STR haplotype database for the Czech population and investigation of its substructure.

    Science.gov (United States)

    Zastera, Jan; Roewer, Lutz; Willuweit, Sascha; Sekerka, Patrik; Benesova, Lucie; Minarik, Marek

    2010-04-01

    Twelve Y-chromosomal short tandem repeats (Y-STR) (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, and DYS439) included in the PowerPlex Y Kit (Promega Corporation, Madison, USA) were studied for 1750 unrelated males living in 14 regions of the Czech Republic. A total of 1148 different haplotypes were found. The overall haplotype diversity (HD) was determined as 0.998. Analysis of Molecular Variance (AMOVA) reveals non-significant distances between regions concerning their haplotype distribution, thus allowing to use the whole sample as a representative reference database of the Czech Republic. Median network analysis shows a remarkable bipartite composition of the Czech haplotypes, falling in distinct clusters with Eastern and Western European roots. PMID:20215022

  15. Analysis of 24 Y chromosomal STR haplotypes in a Chinese Han population sample from Henan Province, Central China.

    Science.gov (United States)

    Shi, Meisen; Liu, Yaju; Zhang, Juntao; Bai, Rufeng; Lv, Xiaojiao; Ma, Shuhua

    2015-07-01

    We analyzed haplotypes for 24 Y chromosomal STRs (Y-STRs), including 17 Yfiler loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DY438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4) and 7 additional STRs (DYS388, DYS444, DYS447, DYS449, DYS522 and DYS527a/b) in 1100 unrelated Chinese Han individuals from Henan Province using AGCU Y24 STR kit systems. The calculated average gene diversity (GD) values ranged from 0.4105 to 0.9647 for the DYS388 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 72.91% with 802 observed haplotypes using 17 Yfiler loci, by the addition of 7 Y-STRs to the Yfiler system, the DC was increased to 79.09% while showing 870 observed haplotypes. Among the additional 7 Y-STRs, DYS449, DYS527a/b, DYS444 and DYS522 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Han origin populations and showed significant differences from other Non-Han populations. In the present study, we report 24 Y-STR population data in Henan Han population, and we emphasize the need for adding additional markers to the commonly used 17 Yfiler loci to achieve more improved discriminatory capacity in a population with low genetic diversity. PMID:25864156

  16. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.38522, Lat: 06.40219 (WGS84); Sensor Depth: 10.10m; Data Date Range: 20100414-20120509.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); Jarvis Island, Pacific Remote Island Areas; Long: -159.99663, Lat: -00.38187 (WGS84); Sensor Depth: 10.10m; Data Date Range: 20100403-20120503.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.03178, Lat: 05.86445 (WGS84); Sensor Depth: 10.10m; Data Date Range: 20100409-20111101.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.03070, Lat: 05.86426 (WGS84); Sensor Depth: 10.10m; Data Date Range: 20100409-20120515.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.12724, Lat: 05.89642 (WGS84); Sensor Depth: 10.40m; Data Date Range: 20100408-20100724.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); South Bank, American Samoa; Long: -170.63274, Lat: -14.89433 (WGS84); Sensor Depth: 32.00m; Data Date Range: 20100306-20120417.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); Asuncion Island, Commonwealth of the Northern Mariana Islands; Long: 145.41759, Lat: 19.69982 (WGS84); Sensor Depth: 12.19m; Data Date Range: 20090424-20110414.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); Alamagan, Commonwealth of the Northern Mariana Islands; Long: 145.81873, Lat: 17.58746 (WGS84); Sensor Depth: 7.01m; Data Date Range: 20090504-20110423.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.37972, Lat: 06.43323 (WGS84); Sensor Depth: 7.90m; Data Date Range: 20100416-20120510.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.38178, Lat: 06.42893 (WGS84); Sensor Depth: 5.20m; Data Date Range: 20100415-20120511.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); Ofu and Olosega Islands, American Samoa; Long: -169.65215, Lat: -14.18020 (WGS84); Sensor Depth: 5.80m; Data Date Range: 20100311-20120425.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); Baker Island, Pacific Remote Island Areas; Long: -176.48860, Lat: 00.19180 (WGS84); Sensor Depth: 9.10m; Data Date Range: 20100207-20120317.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.35927, Lat: 06.38269 (WGS84); Sensor Depth: 6.70m; Data Date Range: 20100417-20120511.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); Ta'u, American Samoa; Long: -169.41830, Lat: -14.22723 (WGS84); Sensor Depth: 12.20m; Data Date Range: 20100313-20120424.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. CRED Subsurface Temperature Recorder (STR); Tutuila, American Samoa; Long: -170.63454, Lat: -14.28829 (WGS84); Sensor Depth: 14.90m; Data Date Range: 20100301-20120326.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  11. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.11363, Lat: 05.86645 (WGS84); Sensor Depth: 19.50m; Data Date Range: 20100408-20120515.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  12. CRED Subsurface Temperature Recorder (STR); Rose Atoll, American Samoa; Long: -168.16864, Lat: -14.54858 (WGS84); Sensor Depth: 8.50m; Data Date Range: 20100304-20120419.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  13. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.12698, Lat: 05.86414 (WGS84); Sensor Depth: 15.50m; Data Date Range: 20100407-20120514.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  14. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.00237, Lat: 05.87611 (WGS84); Sensor Depth: 20.70m; Data Date Range: 20100413-20120515.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  15. CRED Subsurface Temperature Recorder (STR); Jarvis Island, Pacific Remote Island Areas; Long: -160.00835, Lat: -00.36889 (WGS84); Sensor Depth: 32.30m; Data Date Range: 20100401-20120503.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  16. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.37740, Lat: 06.38553 (WGS84); Sensor Depth: 3.70m; Data Date Range: 20100415-20120509.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  17. CRED Subsurface Temperature Recorder (STR); Johnston Atoll, Pacific Remote Island Areas; Long: -169.52672, Lat: 16.74807 (WGS84); Sensor Depth: 2.70m; Data Date Range: 20100126-20120304.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  18. CRED Subsurface Temperature Recorder (STR); Tutuila, American Samoa; Long: -170.66012, Lat: -14.24481 (WGS84); Sensor Depth: 12.50m; Data Date Range: 20100223-20120404.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  19. CRED Subsurface Temperature Recorder (STR); Swains Island, American Samoa; Long: -171.07612, Lat: -11.04560 (WGS84); Sensor Depth: 14.30m; Data Date Range: 20100317-20120322.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  20. CRED Subsurface Temperature Recorder (STR); Rose Atoll, American Samoa; Long: -168.16020, Lat: -14.55127 (WGS84); Sensor Depth: 2.70m; Data Date Range: 20100304-20120418.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  1. CRED Subsurface Temperature Recorder (STR); Ta'u, American Samoa; Long: -169.44109, Lat: -14.21197 (WGS84); Sensor Depth: 12.50m; Data Date Range: 20100312-20120423.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  2. CRED Subsurface Temperature Recorder (STR); Johnston Atoll, Pacific Remote Island Areas; Long: -169.54667, Lat: 16.73276 (WGS84); Sensor Depth: 1.80m; Data Date Range: 20100127-20120305.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  3. CRED Subsurface Temperature Recorder (STR); Kingman Reef, Pacific Remote Island Areas; Long: -162.34221, Lat: 06.39242 (WGS84); Sensor Depth: 7.00m; Data Date Range: 20100414-20120512.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  4. CRED Subsurface Temperature Recorder (STR); Howland Island, Pacific Remote Island Areas; Long: -176.62153, Lat: 00.80646 (WGS84); Sensor Depth: 19.80m; Data Date Range: 20100203-20120312.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  5. CRED Subsurface Temperature Recorder (STR); Aguijan (Goat Is.), Commonwealth of the Northern Mariana Islands; Long: 145.53723, Lat: 14.84772 (WGS84); Sensor Depth: 8.23m; Data Date Range: 20090411-20110503.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  6. CRED Subsurface Temperature Recorder (STR); Ta'u, American Samoa; Long: -169.44661, Lat: -14.25075 (WGS84); Sensor Depth: 6.10m; Data Date Range: 20100313-20120423.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  7. CRED Subsurface Temperature Recorder (STR); Palmyra Atoll, Pacific Remote Island Areas; Long: -162.12811, Lat: 05.89639 (WGS84); Sensor Depth: 11.30m; Data Date Range: 20100408-20110823.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  8. CRED Subsurface Temperature Recorder (STR); Maug, Commonwealth of the Northern Mariana Islands; Long: 145.20750, Lat: 20.02332 (WGS84); Sensor Depth: 17.07m; Data Date Range: 20090429-20110419.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  9. CRED Subsurface Temperature Recorder (STR); Saipan, Commonwealth of the Northern Mariana Islands; Long: 145.76970, Lat: 15.15611 (WGS84); Sensor Depth: 14.63m; Data Date Range: 20090420-20110408.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data from Coral Reef Ecosystem Division (CRED), NOAA Pacific Islands Fisheries Science Center (PIFSC) Subsurface Temperature Recorders (STR) provide a time series...

  10. Population genetic data of the AmpFℓSTR® Identifiler® Plus and PowerPlex® 16 HS STR loci in four Canadian populations.

    Science.gov (United States)

    Laurin, Nancy; Milot, Emmanuel

    2014-03-01

    Allele frequencies and forensically relevant population statistics were estimated for the short tandem repeat (STR) loci of the AmpFℓSTR® Identifiler® Plus and PowerPlex® 16 HS amplification kits, including D2S1338, D19S433, Penta D, and Penta E, for three First Nations Aboriginal populations and for Caucasians in Canada. The cumulative power of discrimination was ≥ 0.999999999999984 and the cumulative power of exclusion was ≥ 0.999929363 for both amplification systems in all populations. No significant departure from Hardy-Weinberg equilibrium was detected for D2S1338, D19S433, Penta D, and Penta E or the 13 Combined DNA Index System core STR loci after correction for multiple testing. Significant genetic diversity was observed between these four populations. Comparison with published frequency data for other populations is also presented.

  11. Allele frequencies and population data for 17 Y-STR loci (The AmpFlSTR® Y-filer™) in Casablanca resident population.

    Science.gov (United States)

    Laouina, Adil; El Houate, Brahim; Yahia, Hakima; Azeddoug, Houssine; Boulouiz, Redouane; Chbel, Faiza

    2011-01-01

    Allele frequencies and population data for 17 Y-STR loci included in the AmpFlSTR® Y-filer™ PCR amplification kit (Applied Biosystems, Foster City, USA), that permit the simultaneous amplification of all the markers included in the actually used European "extended haplotype", DYS19, DYS189I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385I/II, DYS438, DYS439 and also DYS437, DYS448, DYS456, DYS458, DYS635 and Y GATA H4, were obtained from a sample of 166 healthy unrelated males resident in Casablanca (from Morocco). A total of 166 haplotypes were identified, of which 142 were unique. The overall haplotype diversity for the 17 Y-STR loci reached 0.9974, and a discrimination capacity was 0.855. We report some non-standard situations, including duplications and microvariant alleles. PMID:21126935

  12. 利用10个STR-DNA的多态性进行警犬亲子鉴定%Identification of Police Dog Parentage Based on Ten STR-DNA Polymorphisms

    Institute of Scientific and Technical Information of China (English)

    叶俊华; 麻俊武; 杨前勇; 马长书; 郭源梅; 徐黎; 黄路生

    2005-01-01

    采用多重PCR和四色荧光(5′FAM、JOE、NED和ROX)自动化检测技术调查中国警犬种犬PEZ1、FHC2054、FHC2010、PEZ5、PEZ20、PEZ12、PEZ3、PEZ6、PEZ8和FHC2079等10个STR基因座多态性,并计算该10个基因座的等位基因频率(P)、杂合度(H)、多态信息含量(PIC)和非父排除率(PE).实验警犬为7个品种269头种犬.结果显示该10个STR位点的非父排除率(PE)为99.728 7%、个体识别力(DP)为99.999 1%.共调查了7个品种的11个家系48头犬的10个STR基因座上的基因型,建立了警犬STR-DNA亲子鉴定方法.

  13. Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China*

    OpenAIRE

    Wang, Hong-Dan; Shen, Chun-Mei; Liu, Wen-Juan; Zhang, Yu-Dang; Yang, Guang; Yan, Jiang-wei; Qin, Hai-Xia; Zhu, Bo-Feng

    2013-01-01

    We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus, which are not included in the combined DNA index system (CODIS), in a Russian ethnic minority group from the Inner Mongolia Autonomous Region, China. A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system. Using capi...

  14. Mitochondrial DNA and STR analyses for human DNA from maggots crop contents: a forensic entomology case from central-southern China.

    Science.gov (United States)

    Li, X; Cai, J F; Guo, Y D; Xiong, F; Zhang, L; Feng, H; Meng, F M; Fu, Y; Li, J B; Chen, Y Q

    2011-08-01

    Insect larvae and adult insects found on human corpses can provide important forensic evidence however it is useful to be able to prove evidence of association. Without this, it could be claimed that the insect evidence was a contaminant or had been planted on the body. This paper describes how mitochondrial DNA (mtDNA) and STR analysis of the crop contents of larvae of the blowfly Aldrichina grahami collected from separated body parts was used to provide evidence of association.

  15. Allelic frequency distributions of 21 non-combined DNA index system STR loci in a Russian ethnic minority group from Inner Mongolia, China

    Institute of Scientific and Technical Information of China (English)

    Hong-dan WANG; Chun-mei SHEN; Wen-juan LIU; Yu-dang ZHANG; Guang YANG; Jiang-wei YAN; Hai-xia QIN

    2013-01-01

    We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus,which are not included in the combined DNA index system (CODIS),in a Russian ethnic minority group from the Inner Mongolia Autonomous Region,China.A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system.Using capillary electrophoresis,the PCR products of the 21 STR loci were separated and genotyped.A total of 161 alleles were observed in the Russian ethnic minority group,and corresponding allelic frequencies ranged from 0.0044 to 0.5965.The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population's genetic background,for individual identification,and for paternity testing in forensic practice.

  16. Performance testing of a semi-automatic card punch system, using direct STR profiling of DNA from blood samples on FTA™ cards.

    Science.gov (United States)

    Ogden, Samantha J; Horton, Jeffrey K; Stubbs, Simon L; Tatnell, Peter J

    2015-01-01

    The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA(™) sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch. PMID:25407399

  17. Türkiyede y kromozomuna özgü 12 STR lokusu polimorfizmi incelenmesi ve haplotip sıklığının belirlenmesi

    OpenAIRE

    RUSTAMOV, Aydın

    2006-01-01

    Türkiye'de Y Kromozomuna Özgü 12 STR Lokusu Polimorfizmiİncelenmesi ve Haplotip Sıklığının Belirlenmesiİnsan Y-kromozom STR alel sıklıklarının belirlenmesi, dağılımı ve buna bağlı olarak haplotipanalizleri, insan evrimi sırasında gen göçünün arştırılması ve takibinde, erkekler tarafındangerçekleştirilen adli olayların aydınlatılmasında ve babalık tayinlerinde (erkek çocuklar için)gün geçtikçe artan oranda önemli rol oynamaya başlamıştır. Otozomal STR'lerden farklıolarak g...

  18. Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy

    Science.gov (United States)

    Anaya-Segura, Mónica Alejandra; Rangel-Villalobos, Héctor; Martínez-Cortés, Gabriela; Gómez-Díaz, Benjamín; Coral-Vázquez, Ramón Mauricio; Zamora-González, Edgar Oswaldo; García, Silvia; López-Hernández, Luz Berenice

    2016-01-01

    Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disorder in which the detection of female carriers is of the utmost importance for genetic counseling. Haplotyping with polymorphic markers and quantitation of creatine kinase levels (CK) allow tracking of the at-risk haplotype and evidence muscle damage, respectively. Such approaches are useful for carrier detection in cases of unknown mutations. The lack of informative markers and the inaccuracy of CK affect carrier detection. Therefore, herein we designed novel mini-STR (Short Tandem Repeats) assays to amplify 10 loci within the DMD gene and estimated allele frequencies and the polymorphism information content among other parameters in 337 unrelated individuals from three Mexican populations. In addition, we tested the utility of the assays for carrier detection in three families. Moreover, given that serum levels of miR-206 discern between DMD patients and controls with a high area under the curve (AUC), the potential applicability for carrier detection was assessed. The serum levels of miR-206 of non-carriers (n = 24) and carriers (n = 23) were compared by relative quantitation using real-time PCR (p < 0.05), which resulted in an AUC = 0.80 in the Receiver Operating Characteristic curve analysis. In conclusion, miR-206 has potential as a “liquid biopsy” for carrier detection and genetic counseling in DMD. PMID:27529242

  19. Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy

    Directory of Open Access Journals (Sweden)

    Mónica Alejandra Anaya-Segura

    2016-08-01

    Full Text Available Duchenne Muscular Dystrophy (DMD is an X-linked neuromuscular disorder in which the detection of female carriers is of the utmost importance for genetic counseling. Haplotyping with polymorphic markers and quantitation of creatine kinase levels (CK allow tracking of the at-risk haplotype and evidence muscle damage, respectively. Such approaches are useful for carrier detection in cases of unknown mutations. The lack of informative markers and the inaccuracy of CK affect carrier detection. Therefore, herein we designed novel mini-STR (Short Tandem Repeats assays to amplify 10 loci within the DMD gene and estimated allele frequencies and the polymorphism information content among other parameters in 337 unrelated individuals from three Mexican populations. In addition, we tested the utility of the assays for carrier detection in three families. Moreover, given that serum levels of miR-206 discern between DMD patients and controls with a high area under the curve (AUC, the potential applicability for carrier detection was assessed. The serum levels of miR-206 of non-carriers (n = 24 and carriers (n = 23 were compared by relative quantitation using real-time PCR (p < 0.05, which resulted in an AUC = 0.80 in the Receiver Operating Characteristic curve analysis. In conclusion, miR-206 has potential as a “liquid biopsy” for carrier detection and genetic counseling in DMD.

  20. Genetic analysis of eight population groups living in Taiwan using a 13 X-chromosomal STR loci multiplex system.

    Science.gov (United States)

    Hwa, Hsiao-Lin; Lee, James Chun-I; Chang, Yih-Yuan; Yin, Hsiang-Yi; Chen, Ya-Hui; Tseng, Li-Hui; Su, Yi-Ning; Ko, Tsang-Ming

    2011-01-01

    A 13 X-chromosomal short tandem repeat (STR) multiplex system (DXS6807, DXS8378, DSX9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7424, DXS101, GATA172D05, HPRTB, DXS8377, and DXS7423) was tested on 1,037 DNA samples from eight population groups currently living in Taiwan. Different distributions of the allelic frequencies in different populations were presented. DXS8377 and DXS101 were the two most polymorphic loci in these eight populations, whereas DXS7423 was the least informative marker in most of the populations studied. The genetic distances between the populations and the constructed phylogenetic tree revealed a long genetic distance between Asian and Caucasian populations as well as isolation of the Tao population. The phylogenetic tree grouped populations into clusters compatible with their ethnogeographic relationships. This 13 X-chromosomal short tandem repeat multiplex system offers a considerable number of polymorphic patterns in different populations. This system can be useful in forensic identification casework and ethnogeographic research.

  1. Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy.

    Science.gov (United States)

    Anaya-Segura, Mónica Alejandra; Rangel-Villalobos, Héctor; Martínez-Cortés, Gabriela; Gómez-Díaz, Benjamín; Coral-Vázquez, Ramón Mauricio; Zamora-González, Edgar Oswaldo; García, Silvia; López-Hernández, Luz Berenice

    2016-01-01

    Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disorder in which the detection of female carriers is of the utmost importance for genetic counseling. Haplotyping with polymorphic markers and quantitation of creatine kinase levels (CK) allow tracking of the at-risk haplotype and evidence muscle damage, respectively. Such approaches are useful for carrier detection in cases of unknown mutations. The lack of informative markers and the inaccuracy of CK affect carrier detection. Therefore, herein we designed novel mini-STR (Short Tandem Repeats) assays to amplify 10 loci within the DMD gene and estimated allele frequencies and the polymorphism information content among other parameters in 337 unrelated individuals from three Mexican populations. In addition, we tested the utility of the assays for carrier detection in three families. Moreover, given that serum levels of miR-206 discern between DMD patients and controls with a high area under the curve (AUC), the potential applicability for carrier detection was assessed. The serum levels of miR-206 of non-carriers (n = 24) and carriers (n = 23) were compared by relative quantitation using real-time PCR (p < 0.05), which resulted in an AUC = 0.80 in the Receiver Operating Characteristic curve analysis. In conclusion, miR-206 has potential as a "liquid biopsy" for carrier detection and genetic counseling in DMD. PMID:27529242

  2. 利用10个STR-DNA的多态性进行警犬亲子鉴定

    Institute of Scientific and Technical Information of China (English)

    叶俊华; 杨前勇; 马长书; 徐黎; 麻俊武; 郭源梅; 黄路生

    2005-01-01

    采用多重PCR和四色荧光(5’FAM、JOE、NED和ROX)自动化检测技术调查中国警犬种犬PEZ1、FHC2054、FHC2010、PEZ5、PEZ20、PEZ12、PEZ3、PEZ6、PEZ8和FHC2079 等10个STR基因座多态性,并计算该10个基因座的等位基因频率(P)、杂合度(H)、多态信息含量(PIC)和非父排除率(PE)。实验警犬为7个品种269头种犬。结果显示该10个STR位点的非父排除率(PE)为99.7287%、个体识别力(DP)为99.9991%。共调查了7个品种的11个家系48头犬的10个STR基因座上的基因型,建立了警犬 STR-DNA亲子鉴定方法。

  3. Validation of nine non-CODIS STR loci for forensic use in a population from Central Poland.

    Science.gov (United States)

    Kuzniar, Piotr; Jastrzebska, Emilia; Ploski, Rafal

    2006-06-01

    The D7S1517, D3S1744, D12S391, D2S1360, D6S474, D8S1132, D5S2500, D10S2325 and D4S236613 are STR loci potentially useful for forensic purposes whose analysis has recently become facilitated by availability of a commercial kit. The purpose of the study was to evaluate the usefulness of these loci for forensic identification in a population of Central Poland. The distribution of alleles of the nine STRs was determined in sample of 353 unrelated individuals born in Central Poland and indices of forensic informativeness were calculated. The studied loci were highly informative and did not show departures from Hardy-Weinberg equilibrium. For the loci located on the same chromosomes (D2S1360, D3S1744 D4S2366, D5S2500, D7S1517, D8S1132, D12S391) as other loci commonly used for identification purposes (TPOX, D2S1338, D3S1358, FGA, D5S818, D7S820, D8S1179 and D12S391) appropriate pairwise analysis of linkage disequilibrium was performed. In all cases no statistically significant deviation from independence was found. We conclude that the studied STRs are informative and, when necessary, can be used to extend the results obtained with other STRs commonly analyzed for identification purposes, in particular the CODIS set.

  4. Empirical Calibration of the Physical Parameters of Solar-like stars using uvby-Str\\"omgren Photometry

    CERN Document Server

    Mese, Giannina Dalle; Schuster, William J; Chavarría, Carlos; García, J Gabriel

    2012-01-01

    We present an empirical calibration of the physical parameters of A, F and early G-type stars of luminosity class V. We have used a statistical approach based on a sample of about 15 000 stars having both uvby-Str\\"omgren photomerty (Hauck & Mermilliod 1998) and spectral types taken from SIMBAD. Stars closer than 70 pc have been considered reddening-free. In this paper we present the results for 1900 stars within 70 pc. Mean unreddened measurements have been used as input to CHORIZOS (Ma\\'iz-Apell\\'aniz 2004). By assuming "solar metallicity" ([Fe/H]=0), we have been able to determine effective temperatures (Teff) and surface gravities (log g) for each spectral type. We have found a tight correlations among the observations and the derived physical parameters; for example, Teff can be expressed as a cubic polynomial in (b-y)o. From the distribution of colors and indices for 128 stars and their associated physical parameters we have proposed an alternative definition for solar-analogs: stars whose classific...

  5. Developmental Validation of a novel 5 dye Y-STR System comprising the 27 YfilerPlus loci.

    Science.gov (United States)

    Bai, Rufeng; Liu, Yaju; Li, Zheng; Jin, Haiying; Tian, Qinghua; Shi, Meisen; Ma, Shuhua

    2016-01-01

    In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades. PMID:27406339

  6. Haplotype diversity of 17 Y-STR loci in a Chinese Han population sample from Shanxi Province, Northern China.

    Science.gov (United States)

    Bai, Rufeng; Zhang, Zhong; Liang, Quanzeng; Lu, Di; Yuan, Li; Yang, Xue; Shi, Mei sen

    2013-01-01

    The distribution of 17 Y-chromosome STR loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a/b, DYS393, DYS391, DYS439, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, and DYS448 haplotypes was determined in a population sample of 222 unrelated Chinese Han from Shanxi Province, Northern China. A total of 219 haplotypes were observed, and of these, 216 were unique, while 3 were found two times. The overall haplotype diversity was 0.9999 and the discrimination capacity was 0.9865, indicating a high potential for differentiating between male individuals in this population. Comparison analysis via Analysis of Molecular Variance (AMOVA) and construction of MDS plot revealed that Shanxi Han sample clusters with Chinese origin populations and stands far apart of the non-Chinese populations, justifying the establishment of local databases in Shanxi Han population for any future forensic and genetic epidemiology efforts in this region. PMID:23116721

  7. The Y-STR genetic diversity of an Idaho Basque population, with comparison to European Basques and US Caucasians.

    Science.gov (United States)

    Zubizarreta, Josu; Davis, Michael C; Hampikian, Greg

    2011-12-01

    Fifty unrelated Basque males from southwest Idaho were typed for the 17 Y-STR loci in the Yfiler multiplex kit (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, YGATA H4.1 and DYS385a/b). In total, 42 haplotypes were identified, with no more than two individuals sharing a single haplotype. The haplotype diversity (HD) was 0.9935, and gene diversity (D) over loci was 0.457 ± 0.137. The Idaho Basque population was compared to the source population from the Basque autonomous region of Northern Spain and Southern France, as well as a United States Caucasian population. The haplotype diversity for the immigrant Basque sample is within 0.4% of the haplotype diversity of the European Basques (0.9903); thus the power of discrimination is similar for each population. The Idaho Basque population has less diversity in 9 out of 16 loci (considering DYS385a/b together) and 3% less diversity across all loci, compared to the European Basque population. A multidimensional scaling analysis (MDS) was created using pairwise R(ST) values to compare the Idaho Basques to other populations. Based upon R(ST) and F(ST) measures, no significant differentiation was found between the Idaho and source European Basque population. PMID:22276968

  8. Population genetics of 17 Y-STR loci in a large Chinese Han population from Zhejiang Province, Eastern China.

    Science.gov (United States)

    Wu, Weiwei; Pan, Lipeng; Hao, Honglei; Zheng, Xiaoting; Lin, Jinfeng; Lu, Dejian

    2011-01-01

    Seventeen Y-STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS438, DYS439, DYS437, DYS448, DYS456, DYS458, DYS635 and YGATAH4) were analyzed for 4451 Chinese Han unrelated males from Zhejiang Province, Eastern China, with the AmpFlSTR Yfiler™ PCR Amplification kit. A total of 3389 different haplotypes was identified, of which 2877 were unique and 512 repeatedly found among different individuals. The overall haplotype diversity (HD) and discrimination capacity (DC) were 0.999696 and 0.761402, respectively. Analysis of molecular variance (AMOVA) tests demonstrated that genetic distance between Zhejiang Han and most Chinese Han populations is closer than that between Zhejiang Han and non-Han populations. This study provides information for the application of Y-chromosomal STRs to forensic identification, indicating that the extended genotyping of Y-STRs is needed for forensic practice. PMID:20457064

  9. Genome-wide identification of transcriptional start sites in the plant pathogen Pseudomonas syringae pv. tomato str. DC3000.

    Directory of Open Access Journals (Sweden)

    Melanie J Filiatrault

    Full Text Available RNA-Seq has provided valuable insights into global gene expression in a wide variety of organisms. Using a modified RNA-Seq approach and Illumina's high-throughput sequencing technology, we globally identified 5'-ends of transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. A substantial fraction of 5'-ends obtained by this method were consistent with results obtained using global RNA-Seq and 5'RACE. As expected, many 5'-ends were positioned a short distance upstream of annotated genes. We also captured 5'-ends within intergenic regions, providing evidence for the expression of un-annotated genes and non-coding RNAs, and detected numerous examples of antisense transcription, suggesting additional levels of complexity in gene regulation in DC3000. Importantly, targeted searches for sequence patterns in the vicinity of 5'-ends revealed over 1200 putative promoters and other regulatory motifs, establishing a broad foundation for future investigations of regulation at the genomic and single gene levels.

  10. Stavebně technologický projekt bytového domu ve Strážnici

    OpenAIRE

    Rozumek, Jakub

    2015-01-01

    Tématem mé diplomové práce je stavebně technologický projekt bytového domu ve Strážnici. Jedná se o studii realizace hlavních technologických etap objektu SO- 01 Bytový dům, projektu zařízení staveniště, návrhu strojní sestavy, dopravního řešení, technologického postupu provádění zemních prací, základových konstrukcí, pilot CFA a železobetonové stropní konstrukce. Práce obsahuje dále objektový časový plán, finanční plán, podrobný časový plán výstavby bytového domu, rozpočet, kontrolní a zkuše...

  11. An incest case with three biological brothers as alleged fathers: Even 22 autosomal STR loci analysis would not suffice without the mother.

    Science.gov (United States)

    Canturk, Kemal Murat; Emre, Ramazan; Gurkan, Cemal; Komur, Ilhami; Muslumanoglu, Omer; Dogan, Muhammed

    2016-07-01

    Here, we report an incest paternity case involving three biological brothers as alleged fathers (AFs), their biological sister and her child that was investigated using the Investigator ESSplex Plus, AmpFLSTR Identifiler Plus/Investigator IDplex Plus and PowerPlex 16 kits. Initial duo paternity investigations using 15-loci autosomal short tandem repeat (STR) analyses failed to exclude any of the AFs. Despite the fact that one of the brothers, AF1, had a mismatch with the child at a single locus (D2S1338), the possibility of a single-step mutation could not be ruled out. When the number of autosomal STR loci analysed was increased to 22 without the inclusion of the mother, AF2 and AF3 still could not be excluded, since both of them again had no mismatches with the child. A breakthrough was possible only upon inclusion of the mother so that trio paternity investigations were carried out. This time AF1 and AF2 could be excluded at two loci (D2S1338 and D1S1656) and six loci (vWa, D1S1656, D12S391, FGA, PENTA E and PENTA D), respectively, and AF3 was then the only brother who could not be excluded from paternity. Subsequent statistical analyses suggested that AF3 could be the biological father of the child with a combined paternity index >100 billion and a probability of paternity >99.99999999%. These findings consolidate the fact that complex paternity cases such as those involving incest could benefit more from the inclusion of the mother than simply increasing the number of STR loci analysed.

  12. Separation of uncompromised whole blood mixtures for single source STR profiling using fluorescently-labeled human leukocyte antigen (HLA) probes and fluorescence activated cell sorting (FACS).

    Science.gov (United States)

    Dean, Lee; Kwon, Ye Jin; Philpott, M Katherine; Stanciu, Cristina E; Seashols-Williams, Sarah J; Dawson Cruz, Tracey; Sturgill, Jamie; Ehrhardt, Christopher J

    2015-07-01

    Analysis of biological mixtures is a significant problem for forensic laboratories, particularly when the mixture contains only one cell type. Contributions from multiple individuals to biologic evidence can complicate DNA profile interpretation and often lead to a reduction in the probative value of DNA evidence or worse, its total loss. To address this, we have utilized an analytical technique that exploits the intrinsic immunological variation among individuals to physically separate cells from different sources in a mixture prior to DNA profiling. Specifically, we applied a fluorescently labeled antibody probe to selectively bind to one contributor in a mixture through allele-specific interactions with human leukocyte antigen (HLA) proteins that are expressed on the surfaces of most nucleated cells. Once the contributor's cells were bound to the probe, they were isolated from the mixture using fluorescence activated cell sorting (FACS)-a high throughput technique for separating cell populations based on their optical properties-and then subjected to STR analysis. We tested this approach on two-person and four-person whole blood mixtures where one contributor possessed an HLA allele (A*02) that was not shared by other contributors to the mixture. Results showed that hybridization of the mixture with a fluorescently-labeled antibody probe complimentary to the A*02 allele's protein product created a cell population with a distinct optical profile that could be easily differentiated from other cells in the mixture. After sorting the cells with FACS, genetic analysis showed that the STR profile of this cell population was consistent with that of the contributor who possessed the A*02 allele. Minor peaks from the A*02 negative contributor(s) were observed but could be easily distinguished from the profile generated from A*02 positive cells. Overall, this indicates that HLA antibody probes coupled to FACS may be an effective approach for generating STR profiles of

  13. Developmental validation of the Yfiler(®) Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications.

    Science.gov (United States)

    Gopinath, Siddhita; Zhong, Chang; Nguyen, Vivian; Ge, Jianye; Lagacé, Robert E; Short, Marc L; Mulero, Julio J

    2016-09-01

    Y-chromosomal loci have proven useful in solving investigations where low levels of male DNA are present in a high female DNA background. An intrinsic limitation of Y-STRs compared with autosomal STRs is a reduced power of discrimination due to a lack of recombination throughout most of the Y-chromosome. Thus, in an effort to increase the power of discrimination we have developed a new 6-dye, 27-plex Y-STR system that includes the 17 loci from the Yfiler(®) and Yfiler(®) Direct kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 (Y GATA C4), and Y GATA H4) plus three highly polymorphic Y-STR loci (DYS460, DYS481, and DYS533), and seven rapidly mutating Y-STR loci (DYF387S1a/b, DYS449, DYS518, DYS570, DYS576, DYS627) which allow for improved discrimination of related individuals. The Yfiler(®) Plus PCR Amplification Kit is a dual application assay designed to amplify DNA from extracted casework and database samples from storage cards and swab lysates via direct amplification. Compared to the Yfiler PCR Amplification Kit, the new multiplex shows increased discrimination of male lineages and also improved performance in inhibited samples, improved balance in male DNA samples mixed with female DNA at ratios >1:1000, and faster time to results. The Yfiler Plus Kit shows very high concordance to the Yfiler Kit but discordance with the PowerPlex(®) Y23 Kit at the DYS481 locus was observed in 2 out of 30 samples tested. This developmental validation work follows the SWGDAM guidelines and demonstrates that the assay is robust and suitable for use on forensic casework and database samples.

  14. Developmental validation of the Yfiler(®) Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications.

    Science.gov (United States)

    Gopinath, Siddhita; Zhong, Chang; Nguyen, Vivian; Ge, Jianye; Lagacé, Robert E; Short, Marc L; Mulero, Julio J

    2016-09-01

    Y-chromosomal loci have proven useful in solving investigations where low levels of male DNA are present in a high female DNA background. An intrinsic limitation of Y-STRs compared with autosomal STRs is a reduced power of discrimination due to a lack of recombination throughout most of the Y-chromosome. Thus, in an effort to increase the power of discrimination we have developed a new 6-dye, 27-plex Y-STR system that includes the 17 loci from the Yfiler(®) and Yfiler(®) Direct kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 (Y GATA C4), and Y GATA H4) plus three highly polymorphic Y-STR loci (DYS460, DYS481, and DYS533), and seven rapidly mutating Y-STR loci (DYF387S1a/b, DYS449, DYS518, DYS570, DYS576, DYS627) which allow for improved discrimination of related individuals. The Yfiler(®) Plus PCR Amplification Kit is a dual application assay designed to amplify DNA from extracted casework and database samples from storage cards and swab lysates via direct amplification. Compared to the Yfiler PCR Amplification Kit, the new multiplex shows increased discrimination of male lineages and also improved performance in inhibited samples, improved balance in male DNA samples mixed with female DNA at ratios >1:1000, and faster time to results. The Yfiler Plus Kit shows very high concordance to the Yfiler Kit but discordance with the PowerPlex(®) Y23 Kit at the DYS481 locus was observed in 2 out of 30 samples tested. This developmental validation work follows the SWGDAM guidelines and demonstrates that the assay is robust and suitable for use on forensic casework and database samples. PMID:27459350

  15. Retrospective genetic study of germinative mutations in Str loci of individuals potentially exposed to ionizing radiation;Estudo genetico retrospectivo de mutacoes germinativas em Loci Str de individuos potencialmente expostos a radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Costa, Emilia Oliveira Alves

    2010-07-01

    21S11 loeus and 03 mutations on FGA loeus, comprising a total of 11 mutations and a mutation rate of 0.008. In such context, we did not find significant differences (p= 0.15), indicating a possible exposure effect on the mutation rates of the STR loci, in the group accidentally exposed to Cesium-137. (author)

  16. Taxonomy of the Proisotoma complex. VI. Mobile forms of Proisotoma s.str. with the description of a new species from East Siberia (Collembola: Isotomidae).

    Science.gov (United States)

    Potapov, Mikhail; Bogomolov, Maxim

    2016-01-01

    Phenotypic dimorphism of four species of the genus Proisotoma s.str. is described. The modified specimens are characterized by protruding corneas of eyes, stronger pigmentation, enlarged and more chitinized furca, and extension of clavate tenent hairs in the distal part of the tibiotarsi. The characteristics of the modified specimens indicate higher mobile activity. Both sexes are affected by the dimorphism, with the "mobile" forms mostly co-occuring during the cold season with normal specimens. Proisotoma dualis sp. nov. is described from Buryat Republic. PMID:27394339

  17. Studie av subsynkron interaktion (SSTI) mellan ett HVDC system med spänningsstyva strömriktare och en närliggande generator

    OpenAIRE

    Pålsson, Rolf

    2006-01-01

    Syftet med examensarbetet är att studera den subsynkrona interaktionen (subsynchronous torsional interaktion, SSTI) mellan ett HVDC system med spänningsstyva strömriktare och en närliggande generator. Studien utförs med hjälp av datorsimuleringar och fokuserar på att studera dämpkaraktäristiken för SSTI. Studien ska visa hur faktorer såsom den överförda effektens storlek och riktning, vald driftmod och kraftsystemets kortslutningseffekt påverkar dämpkaraktäristiken. Rapporten beskriver subsyn...

  18. Rörliga bilder säger mer än tusen ord : Användning av strömmande media (video) på kommunala hemsidor

    OpenAIRE

    Diaz, German

    2008-01-01

    I min studie har jag undersökt hur strömmande media (video) används på Umeå kommuns webbsida. Dessutom har jag analyserat och jämfört hur sex kommuner i Sverige använder rörliga bilder på sina kommunala hemsidor. De sex kommuner som jag har valt att studera har som gemensamt drag att samtliga sänder respektive kommunfullmäktige live med hjälp av strömmande video. Slutligen diskuterar jag orsaken till att kommunala hemsidor använder strömmande media i liten skala och drar några slutsatser som ...

  19. Monitorování návštěvníků webových stránek

    OpenAIRE

    Jelič, Martin

    2011-01-01

    Práce se zabývá webovou analytikou, jejími pojmy, principy, souvisejícími problémy a jejich řešením. Je zde zevrubně popsáno několik existujících nástrojů pro webovou analytiku. Těžištěm práce je návrh a implementace nového nástroje, který umožňuje monitorovat provoz na webových stránkách a vyhodnocovat údaje za účelem řízení internetových projektů. V rámci práce jsou prezentovány výsledky testování nástroje v reálném provozu a jejich srovnání s existujícími nástroji, od kterých se nový nástr...

  20. Forensic genetic value of a 27 Y-STR loci multiplex (Yfiler(®) Plus kit) in an Italian population sample.

    Science.gov (United States)

    Rapone, Cesare; D'Atanasio, Eugenia; Agostino, Alessandro; Mariano, Martina; Papaluca, Maria Teresa; Cruciani, Fulvio; Berti, Andrea

    2016-03-01

    The analysis of Y chromosome short tandem repeat (Y-STR) haplotypes provides important information that can be used for investigative purposes and in population studies. The Yfiler(®) Plus PCR Amplification kit (Yfiler(®) Plus, Thermo Fisher Scientific, Waltham, MA, USA) allows the multiplex amplification of 27 Y-STRs, including 7 rapidly mutating markers (RM Y-STRs). In this study, 203 unrelated males from Italy, which were subdivided into 4 different geographical groups (North, Center, South and Sardinia) were analyzed. Several intra-population diversity indexes were computed and compared to those obtained using only loci either from the minimal haplotype or the 17-plex (Yfiler(®), Thermo Fisher Scientific, Waltham, MA, USA). In addition, inter-population diversity analysis (RST) among the four Italian samples was performed. The same analysis was also used to compare the Italian sub-sets to other European populations where the Yfiler(®) Plus haplotype frequency data were available. The Sardinians were significantly differentiated from the other three Italian groups, thus requiring a specific sub-national Y-STR haplotype database. The Yfiler(®) Plus kit showed a high power of discrimination which is useful for criminal investigations, principally due to the inclusion of RM Y-STRs.

  1. Styrmir Gunnarsson: Í köldu stríði – Vinátta og barátta á átakatímum

    Directory of Open Access Journals (Sweden)

    Valur Freyr Steinarsson

    2014-12-01

    Full Text Available Í umsögn gagnrýnenda kemur meðal annars eftirfarandi fram: Að mati þess sem hér rýnir er þörf á því að fara betur ofan í saumana á samskiptum Íslendinga við Bandaríkjamenn á tímum kalda stríðsins. Og verður því að taka undir það með bókarhöfundinn að kannski er núna þegar nokkuð er liðið síðan því lauk loksins kominn tími til þess að skoða kalda stríðið á Íslandi frá öllum hliðum.

  2. Determination of mini-short tandem repeat (miniSTR) loci by using the combination of polymerase chain reaction (PCR) and microchip electrophoresis.

    Science.gov (United States)

    Lin, Xuexia; Wu, Jing; Li, Haifang; Wang, Zhihua; Lin, Jin-Ming

    2013-09-30

    In this work, a simple and convenient method for the detection of mini-short tandem repeat (miniSTR) loci has been developed by the combination of polymerase chain reaction (PCR) and microchip electrophoresis (MCE). Degraded or inhibitor DNA greatly limited STR loci analysis. Therefore, The proper primers was designed as close as possible to the STRs region to produce smaller size STRs, and made the assay suitable for the destroyed samples. Two annealing temperatures were applied in one PCR procedure and the corresponding cycle numbers were studied to improve the sensitivity of PCR reaction. Under optimal conditions, 0.001 ng DNA templates were enough to generate miniSTRs. The relative standard deviations (n=3) of the size fifteen miniSTRs from DNA9947A ranged from 0.49% to 4.41%. The RSDs of concentrations were between 0.94% and 4.95%. Fifteen miniSTRs were also well produced from human hair, indicating that the method has great potential application in criminal identification and paternity testing.

  3. Homozygosity mapping in albinism patients using a novel panel of 13 STR markers inside the nonsyndromic OCA genes: introducing 5 novel mutations.

    Science.gov (United States)

    Khordadpoor-Deilamani, Faravareh; Akbari, Mohammad Taghi; Karimipoor, Morteza; Javadi, Gholam Reza

    2016-05-01

    Albinism is a heterogeneous genetic disorder of melanin synthesis that results in hypopigmented hair, skin and eyes. It is associated with decreased visual acuity, nystagmus, strabismus and photophobia. Six genes are known to be involved in nonsyndromic oculocutaneous albinism (OCA). In this study, we aimed to find the disease causing mutations in albinism patients using homozygosity mapping. Twenty three unrelated patients with nonsyndromic OCA or autosomal recessive ocular albinism were recruited in this study. All of the patients' parents had consanguineous marriage and all were screened for TYR mutations previously. At first, we performed homozygosity mapping using fluorescently labeled primers to amplify a novel panel of 13 STR markers inside the OCA genes and then the screened loci in each family were studied using PCR and cycle sequencing methods. We found five mutations including three mutations in OCA2, one mutation in SLC45A2 and one mutation in C10ORF11 genes, all of which were novel. In cases where the disease causing mutations are identical by descent due to a common ancestor, these STR markers can enable us to screen for the responsible genes. PMID:26818737

  4. Y chromosome haplotype diversity in Mongolic-speaking populations and gene conversion at the duplicated STR DYS385a,b in haplogroup C3-M407.

    Science.gov (United States)

    Malyarchuk, Boris A; Derenko, Miroslava; Denisova, Galina; Woźniak, Marcin; Rogalla, Urszula; Dambueva, Irina; Grzybowski, Tomasz

    2016-06-01

    Y chromosome microsatellite (Y-STR) diversity has been studied in different Mongolic-speaking populations from South Siberia, Mongolia, North-East China and East Europe. The results obtained indicate that the Mongolic-speaking populations clustered into two groups, with one group including populations from eastern part of South Siberia and Central Asia (the Buryats, Barghuts and Khamnigans) and the other group including populations from western part of Central Asia and East Europe (the Mongols and Kalmyks). High frequency of haplogroup C3-M407 (>50%) is present in the Buryats, Barghuts and Khamnigans, whereas in the Mongols and Kalmyks its frequency is much lower. In addition, two allelic combinations in DYS385a,b loci of C3-M407 haplotypes have been observed: the combination 11,18 (as well as 11,17 and 11,19) is frequent in different Mongolic-speaking populations, but the 11,11 branch is present mainly in the Kalmyks and Mongols. Results of locus-specific sequencing suggest that the action of gene conversion is a more likely explanation for origin of homoallelic 11,11 combination. Moreover, analysis of median networks of Y-STR haplotypes demonstrates that at least two gene conversion events can be revealed-one of them has probably occurred among the Mongols, and the other event occurred in the Barghuts. These two events give an average gene conversion rate range of 0.24-7.1 × 10(-3) per generation. PMID:26911356

  5. Homozygosity mapping in albinism patients using a novel panel of 13 STR markers inside the nonsyndromic OCA genes: introducing 5 novel mutations.

    Science.gov (United States)

    Khordadpoor-Deilamani, Faravareh; Akbari, Mohammad Taghi; Karimipoor, Morteza; Javadi, Gholam Reza

    2016-05-01

    Albinism is a heterogeneous genetic disorder of melanin synthesis that results in hypopigmented hair, skin and eyes. It is associated with decreased visual acuity, nystagmus, strabismus and photophobia. Six genes are known to be involved in nonsyndromic oculocutaneous albinism (OCA). In this study, we aimed to find the disease causing mutations in albinism patients using homozygosity mapping. Twenty three unrelated patients with nonsyndromic OCA or autosomal recessive ocular albinism were recruited in this study. All of the patients' parents had consanguineous marriage and all were screened for TYR mutations previously. At first, we performed homozygosity mapping using fluorescently labeled primers to amplify a novel panel of 13 STR markers inside the OCA genes and then the screened loci in each family were studied using PCR and cycle sequencing methods. We found five mutations including three mutations in OCA2, one mutation in SLC45A2 and one mutation in C10ORF11 genes, all of which were novel. In cases where the disease causing mutations are identical by descent due to a common ancestor, these STR markers can enable us to screen for the responsible genes.

  6. Genetic Polymorphisms of 21 Non-CODIS STR Loci%21个非CODIS STR基因座的遗传多态性

    Institute of Scientific and Technical Information of China (English)

    邵伟波; 张素华; 李莉

    2011-01-01

    Objective To investigate genetic polymorphisms of 21 non-CODIS STR loci in Han population from the east of China and to explore their forensic application value. Methods Twenty-one non-CODIS STR loci, were amplified with AGCU 21+1 STR kit and DNA samples were obtained from 225 unrelated individuals of the Hah population from the east of China. The PCR products were analyzed with 3130 Genetic Analyzer and genotyped with GeneMapper ID v3.2 software. The genetic data were statistically analyzed with PowerStats vl2.xls and Cervus 2.0 software. Results The distributions of 21 non-CODIS STR loci satisfied the Hardy-Weinberg equilibration. The heterozygosity (H) distributions were 0.596-0.804, the diserimination power (DP) were 0.764-0.948, the probability of exclusion of duo-testing (PEduo) were 0.1760.492, the probability of exclusion of trios-testing (PEso) were 0.334-0.663, and the polymorphic information content (PIC) were 0.522-0.807. The cumulative probability of exclusion (CPE) of duo-testing was 0.999 707, the CPE of trios-testing was 0.999 9994, and the cumulated discrimination power (CDP) was 0.99999999999999999994. Conclusion Twenty-one non-CODIS STR loci are highly polymorphie. They can be effectively used in personal identification and paternity testing in trios cases. They can also be used as supplement in the difficult cases of diad paternity testing.%目的 调查华东汉族人群21个非CODIS STR基因座的遗传多态性并评价其法医学应用价值.方法 用AGCU 21+1 STR试剂盒,对华东地区汉族225个无关个体的21个非CODIS STR基因座进行扩增,用3130遗传分析仪检测扩增产物,GeneMapper ID v3.2软件进行分型,采用PowerStats v12.xls和Cervus 2.0分析软件计算常用法医遗传学参数.结果 21个非CODIS STR基因座的频率分布在本组人群中均符合Hardy-Weinberg平衡.杂合度分布为0.596~0.804,个体识别率为0.764~0.948,二联体非父排除率(PEduo)为0.176~0.492,三

  7. Multiple Scales in Fluid Dynamics and Meteorology: The DFG Priority Programme 1276 MetStröm

    Science.gov (United States)

    von Larcher, Th; Klein, R.

    2012-04-01

    -temporal grid adaptivity get invoked in order to further improve the net efficiency in exploiting the given computational resources. In the setting of geophysical flow simulation one must then employ subgrid scale parameterizations that dynamically adapt to the changing grid sizes and time steps, implement ways to judiciously control and steer the newly available flexibility of resolution, and invent novel ways of quantifying the remaining errors. The DFG priority program MetStröm covers the expertise of Meteorology, Fluid Dynamics, and Applied Mathematics to develop model- as well as grid-adaptive numerical simulation concepts in multidisciplinary projects. The goal of this priority programme is to provide simulation models which combine scale-dependent (mathematical) descriptions of key physical processes with adaptive flow discretization schemes. Deterministic continuous approaches and discrete and/or stochastic closures and their possible interplay are taken into consideration. Research focuses on the theory and methodology of multiscale meteorological-fluid mechanics modelling. Accompanying reference experiments support model validation.

  8. ldentification of the relationship of orphaned sisters by combined using autosomal STR, X-STR and mitochondrial-SNP%联合应用常染色体STR、X-STR和线粒体SNP鉴别缺失双亲姐妹同胞关系

    Institute of Scientific and Technical Information of China (English)

    王晓勋; 陈敏; 李瑞明; 贺娇; 王彦涛; 罗银州

    2014-01-01

    目的:对双亲缺失的姐妹进行同胞鉴定。方法采用chelex-100法提取血液DNA ,通过检测常染色体短串联重复序列(short tandem repeat, STR)位点、X 染色体STR位点和线粒体单核苷酸多态性(single nucleotide polymorphism, SNP)进行基因分型,分型结果通过判别函数、ITO 法、X 染色体STR位点和线粒体SNP分析的方法进行同胞关系判定。结果39个常染色体等位基因位点的共享基因数为27个,用辨别函数判别归于无关个体;用ITO 法计算亲缘关系系数(FSI)为6.95003×10-44,判别为无关个体;12个X 染色体 STR 等位基因位点中,有3个X 染色体STR位点不匹配,排除姐妹俩来自同一父亲;线粒体SNP的检测显有3个基因座不同,两人来源于不同母系。结论通过联合利用常染色体STR、X染色体STR和线粒体SNP检测技术进行确认两人不是同胞姐妹关系。%Objective To carry out a sibling identification on the orphaned sisters. Methods Genomic DNA of two ladies were extracted by using chelex-100 method. Genotyping was performed by detecting STRs loci on autosome, STR loci on X-chromosome and mitochondrial-SNP loci. Then the data were analyzed by discriminant function analysis , ITO method , X-STR analysis and mitochondrial-SNP analysis to identify the sibling relation of the orphaned sisters. Results There were 27 shared loci among 39 STRs loci on the autosome , which were considered as unrelated individuals based on discriminant function analysis and ITO method. 3 inconsistent loci were detected among 12 X-STR loci , which implied that the two sisters didn’t have a biological father. 3 inconsistent loci were detected among 49 mitochondrial-SNP loci, which suggested that they didn’t have a biological mother. Conclusion The joint use of the technology of autosome STR , X-chromosome STR and mitochondrial-SNP determined that two ladies were not sibling relationship.

  9. Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome-Targeted Pre-amplification and Next Generation Y-STR Amplification Systems.

    Science.gov (United States)

    Hanson, Erin K; Ballantyne, Jack

    2016-01-01

    In some cases of sexual assault the victim may not report the assault for several days after the incident due to various factors. The ability to obtain an autosomal STR profile of the semen donor from a living victim rapidly diminishes as the post-coital interval is extended due to the presence of only a small amount of male DNA amidst an overwhelming amount of female DNA. Previously, we have utilized various technological tools to overcome the limitations of male DNA profiling in extended interval post-coital samples including the use of Y-chromosome STR profiling, cervical sample, and post-PCR purification permitting the recovery of Y-STR profiles of the male DNA from samples collected 5-6 days after intercourse. Despite this success, the reproductive biology literature reports the presence of spermatozoa in the human cervix up to 7-10 days post-coitus. Therefore, novel and improved methods for recovery of male profiles in extended interval post-coital samples were required. Here, we describe enhanced strategies, including Y-chromosome-targeted pre-amplification and next generation Y-STR amplification kits, that have resulted in the ability to obtain probative male profiles from samples collected 6-9 days after intercourse.

  10. A NEW SPECIES OF THE GENUS TOMOCERUS (S. STR. ) ( COLLEMBOLA: TOMOCERIDAE ) FROM CHINA%中国鳞属Tomocerus 1新种 (弹尾目:鳞科)

    Institute of Scientific and Technical Information of China (English)

    刘永琴

    2006-01-01

    In this paper a new species of Collembola, Tomocerus (s. str. ) jinyunensis of the genus Tomocerus Nicolet 1841 in China, is described. It is similar to Tomocerus (s. str.) jesonicus Yosii 1967, but differs from the latter in its tibiotarsus color, ungui inner teeth, tibiotarsal blunt spiny setae and scale color. All the type specimens are deposited in the College of Life and Engineering, Southwest University of Science and Technology.%记述了中国鳞属Tomocerus Nicolet 1841 1新种--缙云鳞Tomocerus(s.str.)jinyunensis,sp.nov..该新种与杰氏鳞Tomocerus(s.str.)jesonicus Yosii 1967很相似,但可以从胫跗节颜色、爪内缘齿数、胫跗节粗钝刺状大刚毛及鳞片颜色等特征相区别.模式标本保存于西南科技大学生命科学与工程学院昆虫标本室.

  11. An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection

    Science.gov (United States)

    Elkins, Kelly M.; Kadunc, Raelynn E.

    2012-01-01

    In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for validation and various student-designed short tandem repeat (STR) primers for Combined DNA Index System (CODIS) loci. SYBR Green was used to detect the amplification of the expected amplicons. The…

  12. Modelling noise in second generation sequencing forensic genetics STR data using a one-inflated (zero-truncated) negative binomial model

    DEFF Research Database (Denmark)

    Vilsen, Søren B.; Tvedebrink, Torben; Mogensen, Helle Smidt;

    2015-01-01

    We present a model fitting the distribution of non-systematic errors in STR second generation sequencing, SGS, analysis. The model fits the distribution of non-systematic errors, i.e. the noise, using a one-inflated, zero-truncated, negative binomial model. The model is a two component model....... The first component models the excess of singleton reads, while the second component models the remainder of the errors according to a truncated negative binomial distribution. We estimated the parameters of the model in two ways: (1) we maximised the likelihood using an explicitly calculated gradient...... function and (2) we used the expectation-maximisation, EM, algorithm. The estimated parameters were used to create dynamic, sample specific thresholds for noise removal using marker specific proportions of the negative binomial distribution. Based on data from dilution series experiments (amounts of DNA...

  13. Fluorescence-based thermal shift data on multidrug regulator AcrR from Salmonella enterica subsp. entrica serovar Typhimurium str. LT2

    Directory of Open Access Journals (Sweden)

    Babu A. Manjasetty

    2016-06-01

    Full Text Available The fluorescence-based thermal shift (FTS data presented here include Table S1 and Fig. S1, and are supplemental to our original research article describing detailed structural, FTS, and fluorescence polarization analyses of the Salmonella enterica subsp. entrica serovar Typhimurium str. LT2 multidrug transcriptional regulator AcrR (StAcrR (doi:10.1016/j.jsb.2016.01.008 (Manjasetty et al., 2015 [1]. Table S1 contains chemical formulas, a Chemical Abstracts Service (CAS Registry Number (CAS no., FTS rank (a ligand with the highest rank has the largest difference in the melting temperature (ΔTm, and uses as drug molecules against various pathological conditions of sixteen small-molecule ligands that increase thermal stability of StAcrR. Thermal stability of human enolase 1, a negative control protein, was not affected in the presence of various concentrations of the top six StAcrR binders (Fig. S1.

  14. Automated extraction of DNA from blood and PCR setup using a Tecan Freedom EVO liquid handler for forensic genetic STR typing of reference samples

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Frøslev, Tobias G; Frank-Hansen, Rune;

    2011-01-01

    We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, Männedorf, Switzerland). The protocols were validated for accredited forensic genetic work according to ISO...... 17025 using the Qiagen MagAttract DNA Mini M48 kit (Qiagen GmbH, Hilden, Germany) from fresh whole blood and blood from deceased individuals. The workflow was simplified by returning the DNA extracts to the original tubes minimizing the risk of misplacing samples. The tubes that originally contained...... the samples were washed with MilliQ water before the return of the DNA extracts. The PCR was setup in 96-well microtiter plates. The methods were validated for the kits: AmpFlSTR Identifiler, SGM Plus and Yfiler (Applied Biosystems, Foster City, CA), GenePrint FFFL and PowerPlex Y (Promega, Madison, WI...

  15. 应用DMD基因内含子49上STR-PCR分析进行DMD/BMD杂合子携带者的诊断

    Institute of Scientific and Technical Information of China (English)

    胡冬贵; 华小云; 林群娣; 杜传书

    1995-01-01

    DMD基因内含子49上的STR-PCR产物经8%中性PAGE电泳银染显色。我们获得满意的DMD/BMD杂合子携带者的诊断。这样不但避免了同位素的应用,而且摆脱了变性PAGE中脲素对银染显色的影响,从而使DMD基因的STR—PCR技术更加简便易行,结果易于分析,利于通过STR—PCR风险单体型的分析达到快速诊断DMD/BMD患者和携带者的目的.

  16. Haplotype data and mutation rates for the 23 Y-STR loci of PowerPlex® Y 23 System in a Northeast Italian population sample.

    Science.gov (United States)

    Turrina, Stefania; Caratti, Stefano; Ferrian, Melissa; De Leo, Domenico

    2015-07-01

    The PowerPlex® Y 23 System (Promega) is a short tandem repeat (STR) multiplex that allows co-amplification of 23 gonosomal Y-STRs, combining 17 loci commonly included in commercially available kits (DYS389I, DYS448, DYS389II, DYS19, DYS391, DYS438, DYS437, DYS635, DYS390, DYS439, DYS392, DYS393, DYS458, DYS385a/b, DYS456, and Y-GATA-H4) and six new loci (DYS481, DYS549, DYS533, DYS643, DYS576, and DYS570) with the last two being rapidly mutating Y-STRs (RM Y-STRs). In order to assess the possible gain in forensic efficiency provided by the six additional markers, a population sample of 410 unrelated healthy males originating from Northeast Italy (Veneto, Trentino Alto Adige, Lombardia, and Friuli Venezia Giulia regions) was typed. The data (335 of the 410 samples) are available in the Y chromosome haplotype reference database under accession number YA003327. Overall, 410 unique haplotypes were found corresponding to a global haplotype diversity (HD) of 0.999994 with a discriminatory capacity (DC) of 100%. Allelic microvariants, null alleles, and duplications were detected. Pairwise genetic distances (R(ST)) calculated among neighboring European reference populations revealed no significant differences. Furthermore, for studying Y-STR mutation rates, 90 father-son pairs, in which the fathers were already included in the full dataset, were tested. On a total of 2,070 meioses considered, eight single-step mutational events were observed, two of which within the same father-son pair and the average mutation rate was 3.38 × 10(-3) per locus per generation (95% confidence interval, 1.36 × 10(-3)-6.95 × 10(-3)). PMID:25099381

  17. A study of the thermophilic ribosomal protein S7 binding to the truncated S12-S7 intercistronic region provides more insight into the mechanism of regulation of the str operon of E. coli(1).

    Science.gov (United States)

    Spiridonova, V A; Rozhdestvensky, T S; Kopylov, A M

    1999-10-29

    A study of the ability of His6-tagged ribosomal protein S7 of Thermus thermophilus to interact with the truncated S12-S7 intercistronic region of str mRNA of Escherichia coli has been described. A minimal S7 binding mRNA fragment is a part of the composite hairpin, with the termination codon of the S12 cistron on one side and the initiation codon of the next S7 cistron on the other. It has a length in the range of 63-103 nucleotides. The 63 nucleotide mRNA fragment, which corresponds to a putative S7 binding site, binds very poorly with S7. Tight RNA structure models, which behave as integral systems and link the S7 binding site with the translational regulation region of the hairpin, are suggested. This observation provides more insight into the mechanism of S7-directed autogenous control of translational coupling of str mRNA.

  18. Population studies for STR loci (D3S1358, D5S818, D7S820, D18S51 and FGA) in NWFP and Sindhi populations of Pakistan for forensic use.

    Science.gov (United States)

    Saqib Shahzad, M; Abbas Bokhari, S Yassir; Rao, Abdul Qayyum; Raza, M Hashim; Ullah, Obaid; Zia-Ur-rahman; Shahid, A Ali; Ahmad, Zahoor; Riazuddin, S

    2004-01-01

    CEMB's Forensic DNA typing project is directed towards the introduction of DNA typing technique in Pakistan's criminal justice system so as to exonerate an innocent and wrongly accused person and incriminates the culprit. The present study is a part of the project of CEMB to analyze Sindhi and NWFP (North West Frontier Province) populations for five STR (Short Tandem Repeat) loci out of 13 CODIS (Combined DNA Index System) loci. Allelic frequencies and heterozygosity for STR markers D3S1358, D5S818, D7S820, D18S51 and FGA (FIBRA) were determined. Samples from unrelated individuals were amplified by multiplex PCR using the unlabelled primers for these markers followed by denaturing Polyacrylamide Gel Electrophoresis (PAGE). Statistical analysis was performed to determine the allelic frequencies and was evaluated using the Chi Square Test. PMID:15782779

  19. Application of STR genetic marker system in the detection of hemophilia A carriers in Guangxi, China%STR遗传标记在广西地区血友病A携带者诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    周峻荔; 韦红英; 吴华; 胡艳玲; 梁伟玲

    2012-01-01

    Objective To establish a fast and simple genetic diagnosis technique based on a reliable, short tandem repeat (STR) genetic marker system for the detection of hemophilia A carriers in Guangxi, China. Methods Fluorescent PCR and + capillary electrophoresis were used for allele genotyping at three intragenic/extragenic STR loci (F81ntl3, DXS1073, and DXS9901) of FVIII gene in the members of 10 hemophilia A families in Guangxi, so as to evaluate the diagnostic efficiency of the STR genetic marker system for detection of hemophilia A carriers. Then the STR genetic marker system was used to detect hemophilia A carriers among examinees. Results In the 10 hemophilia A families, 11 confirmed female carriers had the same allele fragment lengths at the three STR loci (F8Intl3, DXS1O73, and DXS9901) as the probands. Of the 8 females examined, 5 had allele fragments at the three STR loci (F8Intl3, DXS1073, and DXS9901) which were identical to those of the probands, and thus they were diagnosed as hemophilia A carriers. Conclusions Genetic analysis at the three STR loci (F8Intl3, DXS1073, and DXS9901) can be used to detect hemophilia A carriers rapidly and provide reliable basis for prenatal diagnosis of hemophilia A.%目的 建立理想的STR 遗传标记体系,对广西地区血友病A携带者进行快速简便的基因诊断.方法 选取广西地区10个血友病A家系作为研究对象,运用荧光PCR联合毛细管电泳的方法,对家系成员中FⅧ基因内外具有高度遗传性的3个STR位点F8Int13、DXS1073、DXS9901进行等位基因分型,评估该体系用于家系中血友病A携带者的诊断效率,并对待检者进行携带者诊查.结果 10个血友病A家系中,11例肯定女性携带者均含有与相应先证者完全一致的3个STR等位基因(F8Int13、DXS1073、DXS9901)片段长度;在待检的8例女性中,5例检出3个STR等位基因片段与相应家系中先证者完全相同,被诊断为血友病A携带者.结论 联合应用3

  20. Effects of Matrine to Stenus (Stenus, s.str)sp.1 and Paedenus fuscipes Curtis%苦参碱对大眼隐翅虫和梭毒隐翅虫的影响

    Institute of Scientific and Technical Information of China (English)

    肖凡; 郑发科

    2005-01-01

    大眼隐翅虫Stenus ( Stenus, s.str ) sp.1 和梭毒隐翅虫Paedenus fuscipes Curtis是重要的天敌昆虫.用苦参碱在室内做了大眼隐翅虫和梭毒隐翅虫的药效实验.结果表明,苦参碱对大眼隐翅虫和梭毒隐翅虫有一定的毒杀作用.