WorldWideScience

Sample records for arkansas power-lsenic 71

  1. Slamming Arkansas Schools!

    Science.gov (United States)

    Scott, W. Clayton

    2010-01-01

    In this article, the author, a poet and teaching artist, shares how he successfully brought slam poetry to College Hill Middle School in Texarkana, Arkansas. In 2001 he discovered slam poetry--a poetry-reading format in which poets compete in dramatic readings of their works--and went to Slam Nationals in Seattle on the Arkansas slam team. He…

  2. Census Snapshot: Arkansas

    OpenAIRE

    Romero, Adam P; Baumle, Amanda K; Badgett, M. V. Lee; Gates, Gary J.

    2007-01-01

    Using data from the U.S. Census Bureau, this report provides demographic and economic information about same-sex couples and same-sex couples raising children in Arkansas. We compare same-sex “unmarried partners,” which the Census Bureau defines as an unmarried couple who “shares living quarters and has a close personal relationship,” to different-sex married couples in Arkansas. In many ways, the almost 5,900 same-sex couples living in Arkansas are similar to married couples. Accord...

  3. Arkansas' Disappearing Tax Base.

    Science.gov (United States)

    Schoppmeyer, Martin; Venters, Tommy

    State legislation that has contributed to the reduction of Arkansas' tax base is described in this paper. Amendment 59, adopted in 1980, has reduced the state tax base by millions of dollars. At the end of 1992, the majority of school districts have equalized their real, personal, and carrier and utility property. Act 34, the current foundation…

  4. 27 CFR 9.112 - Arkansas Mountain.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Arkansas Mountain. 9.112... Arkansas Mountain. (a) Name. The name of the viticultural area described in this section is “Arkansas Mountain.” (b) Approved maps. The appropriate maps for determining the boundary of the Arkansas...

  5. Arkansas River Water Needs Assessment

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report is on the legal elements, hydrologic analysis, objectives, and water levels related to the Arkansas River and the management of it.

  6. Marshallese COFA Migrants in Arkansas.

    Science.gov (United States)

    McElfish, Pearl Anna

    2016-05-01

    Arkansas is home to one of the largest populations of Marshallese in the world. Marshallese communities suffer from a disproportionate incidence of chronic diseases, including obesity, cardiovascular disease, diabetes, and infectious diseases, such as Hansen's disease (leprosy), tuberculosis, and types of hepatitis. There are a number of structural, legal, economic, and social issues that must be addressed in order to reduce health disparities and increase access to health care for Marshallese living in Arkansas. PMID:27263176

  7. 33 CFR 117.121 - Arkansas River

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Arkansas River 117.121 Section 117.121 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Arkansas § 117.121 Arkansas River The draw of...

  8. Arkansas Groundwater-Quality Network

    Science.gov (United States)

    Pugh, Aaron L.; Jackson, Barry T.; Miller, Roger

    2014-01-01

    Arkansas is the fourth largest user of groundwater in the United States, where groundwater accounts for two-thirds of the total water use. Groundwater use in the State increased by 510 percent between 1965 and 2005 (Holland, 2007). The Arkansas Groundwater-Quality Network is a Web map interface (http://ar.water.usgs.gov/wqx) that provides rapid access to the U.S. Geological Survey’s (USGS) National Water Information System (NWIS) and the U.S. Environmental Protection Agency’s (USEPA) STOrage and RETrieval (STORET) databases of ambient water information. The interface enables users to perform simple graphical analysis and download selected water-quality data.

  9. Arkansas Tech University reactor project

    International Nuclear Information System (INIS)

    The purpose of this paper is to describe the nuclear reactor project at Arkansas Tech University (ATU). The reactor will be a part of the Center for Energy Studies (CES) located on the ATU campus in Russellville, Arkansas. It will be used for education, training, and research. The Arkansas Tech University TRIGA Reactor (ATUTR) is a TRIGA Mark I that will be operated in two basic modes: steady state and pulsing. The maximum power level for steady-state operation is 250 kW(thermal), and the maximum step reactivity insertion will be 2.0$. The inherent safety of this reactor comes from the large negative temperature coefficient of the uranium-zirconium-hydride fuel-moderator elements. Principal design parameters for the reactor are summarized. The application for reactor construction and operating license was submitted to the US Nuclear Regulatory Commission in November 1989. The review process is continuing. ATU plans to continue to work with local industry and the public to provide a wide variety of services related to nuclear science and engineering

  10. Arkansas: Its Land and People, Vol. 1.

    Science.gov (United States)

    Foti, Tom

    This bicentennial volume offers a blend of Arkansas history, ecology, and literature. Its purpose is to show the relationship between Arkansas' people and their land. It contains a discussion of the Natural Divisions concept, the geological development of the state, and descriptions by early pioneers. The volume is not intended to be a complete…

  11. Goose survey, Arkansas, December 15 - 19, 1980

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and BlueSnow Geese are included.

  12. Arkansas' Children: How Well Are They Doing? Arkansas Kids Count Data Book 1996.

    Science.gov (United States)

    Dahlstrom, Sherryl M.; High, Rhonda L.

    This Kids Count data book is the fourth to examine the well-being of Arkansas' children and notes trends from 1990 to 1995. The report's introductory sections include discussions of the potential impact of welfare reform on Arkansas' children, and present figures detailing the number of children affected by particular risk factors each week. The…

  13. Arkansas' Junior Executive Training Curriculum Guide.

    Science.gov (United States)

    Clayton, Dean; And Others

    This curriculum guide is designed to help teachers conduct courses on small business management and entrepreneurship to high school seniors in Arkansas. The program focuses upon the managerial process, examining the functions of planning, organization, staffing, directing, and controlling as related to the activities and responsibilities of the…

  14. Rickettsiae in Gulf Coast Ticks, Arkansas, USA

    OpenAIRE

    Trout, Rebecca; Steelman, C Dayton; Szalanski, Allen L.; Williamson, Phillip C

    2010-01-01

    To determine the cause of spotted fever cases in the southern United States, we screened Gulf Coast ticks (Amblyomma maculatum) collected in Arkansas for rickettsiae. Of the screened ticks, 30% had PCR amplicons consistent with Rickettsia parkeri or Candidatus Rickettsia amblyommii.

  15. Arkansas Physical Education Curriculum Framework, 1996.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This K-12 physical education curriculum presents six areas in which Arkansas students should show competence. The six strands are: health related fitness (students will be knowledgeable of and derive immediate health benefits from participation in vigorous musculoskeletal and cardiorespiratory enhancing activities); movement/motor skills (students…

  16. 78 FR 5202 - Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR

    Science.gov (United States)

    2013-01-24

    ... National Park Service Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Arkansas State University Museum... culturally affiliated with the human remains may contact the Arkansas State University Museum....

  17. 78 FR 5199 - Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR

    Science.gov (United States)

    2013-01-24

    ... National Park Service Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Arkansas State University Museum... associated funerary objects may contact the Arkansas State University Museum. Repatriation of the...

  18. Time of travel of selected Arkansas streams

    Science.gov (United States)

    Lamb, T.E.

    1982-01-01

    Between 1971 and 1981, time-of-travel and dispersion measurements were made in 15 streams in Arkansas. Most of the streams studied were at or near base flow. Graphs are presented for predicting traveltime of solutes in segments of the streams studied. The relationship of time of passage and peak unit concentration to traveltime is presented for two of the streams. Examples of use and application of the data are given. (USGS)

  19. Annual yield and selected hydrologic data for the Arkansas River basin compact Arkansas-Oklahoma, 1995 water year

    Science.gov (United States)

    Porter, J.E.

    1996-01-01

    The computed annual yield and deficiency of the subbasins as defined in the Arkansas River Basin Compact, Arkansas-Oklahoma, are given in tables for the 1995 water year. Actual runoff from the subbasins and depletion caused by major reservoirs in the compact area also are given in tabular form. Monthly mean discharges are shown for the 17 streamflow stations used in computing annual yield. Water-quality data are shown for 20 water-quality stations sampled in the Arkansas River Basin.

  20. Annual yield and selected hydrologic data for the Arkansas River Basin Compact, Arkansas-Oklahoma, 1996 water year

    Science.gov (United States)

    Porter, J. Elton

    1997-01-01

    The computed annual yield and deficiency of the subbasins as defined in the Arkansas River Basin Compact, Arkansas-Oklahoma, are given in tables for the 1996 water year. Actual runoff from the subbasins and depletion caused by major reservoirs in the compact area also are given in tabular form. Computed monthly mean discharges are shown for the 21 streamflow stations in the Arkansas River Basin. Water-quality data are shown for 16 water-quality stations sampled in the Arkansas River Basin.

  1. Success For Arkansas: Success for All Schools In Arkansas Continue Gains On Benchmark Exams

    Science.gov (United States)

    Success for All Foundation, 2004

    2004-01-01

    Success for All is the most extensively researched of all comprehensive reform models for Title I elementary schools. It incorporates scientifically based principles of reading, cooperative learning, professional development, tutoring, and family support. Arkansas elementary schools using the Success for All reading program continued to make …

  2. A Look at Arkansas' Children: Arkansas Kids Count Data Book 1997.

    Science.gov (United States)

    Dahlstrom, Sherryl M.; High, Rhonda L.

    This Kids Count data book provides information on indicators of the well-being of Arkansas' children. The report's introduction discusses factors contributing to increased attention to children and families, the need for broad-based community collaborations to address children and family needs, and cultural changes influencing families. Data…

  3. Arkansas' Anemometer Loan Program

    Energy Technology Data Exchange (ETDEWEB)

    Fernando Vego

    2012-10-11

    The measurement campaign had one year duration from 04/01/2011 to 03/31/2012 and was taken at 20m and 34m with NRG instrumentation. The data was analyzed weekly to check inconsistencies and validity and processed using Excel, Flexpro and Windographer standard Edition Version 2.04. The site analyzed is located in the Waldron, Arkansas in Scott County. It is an open site for most of the direction sectors with immediate roughness class of 1.5. It has seasonally directional winds, of which the most energetic come from the southern direction. The vertical wind profile shows moderate wind shear that varies by season as well.

  4. Small Works in Arkansas: How Poverty and the Size of Schools and School Districts Affect School Performance in Arkansas. Summary of Recent Research.

    Science.gov (United States)

    Rural School and Community Trust, Washington, DC.

    A study examined how Arkansas students' achievement is related to poverty, school and district size, and the interaction between these factors. Achievement test scores from grades 4, 5, 7, 8, and 9 in all Arkansas schools were supplied by the Arkansas Department of Education. Poverty levels were determined by percentage of students receiving…

  5. Annual yield and selected hydrologic data for the Arkansas River Basin Compact, Arkansas-Oklahoma, 1994 water year

    Science.gov (United States)

    Porter, J.E.

    1995-01-01

    The computed annual yield and deficiency of the subbasins as defined in the Arkansas River Basin Compact, Arkansas-Oklahoma, are given in tables for the 1994 water year. Actual runoff from the subbasins and depletion caused by major reservoirs in the compact area also are given in tabular form. Monthly maximum, minimum, and mean discharges are shown for the 14 streamflow stations used in computing annual yield. Water-quality data are shown for 11 water-quality stations sampled in the Arkansas River Basin.

  6. Annual yield and selected hydrologic data for the Arkansas River Basin Compact Arkansas-Oklahoma 1993 water year

    Science.gov (United States)

    Porter, J.E.; Barks, C. Shane

    1994-01-01

    The computed annual yield and deficiency of the subbasins as defined in the Arkansas River Basin Compact, Arkansas-Oklahoma, are given in tables for the 1993 water year. Actual runoff from the subbasins and depletion caused by major reservoirs in the compact area also are given in tabular form. Monthly maximum, minimum, and mean discharges are shown for the 14 streamflow stations used in computing annual yield. Water-quality data are shown for 12 water-quality stations sampled in the Arkansas River Basin.

  7. Final Critical Habitat for the Arkansas River Shiner (Notropis girardi)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Arkansas River Shiner (Notropis girardi) occur. The geographic extent includes New...

  8. Arkansas: 1986 all goose survey: December 14 - 18, 1987

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  9. Central Arkansas National Wildlife Refuge Complex: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Central Arkansas NWR Complex for the next 15 years. This plan outlines the Complex...

  10. 76 FR 44031 - Arkansas; Major Disaster and Related Determinations

    Science.gov (United States)

    2011-07-22

    ....046, Fire Management Assistance Grant; 97.048, Disaster Housing Assistance to Individuals and...--Disaster Housing Operations for Individuals and Households; 97.050, Presidentially Declared Disaster... SECURITY Federal Emergency Management Agency Arkansas; Major Disaster and Related Determinations...

  11. [Arkansas annual winter goose survey: December 10 & 11, 1979

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, resident geese, and BlueSnow Geese are included.

  12. [Arkansas annual winter goose survey: December 11 - 15, 1978

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, resident geese, and BlueSnow Geese are included.

  13. 1985 goose survey: December 9 - 13, 1985: Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  14. 1982 goose survey: December 13 - 17, 1982: Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  15. 1983 goose survey: December 12 - 16, 1983: Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  16. Arkansas: 1986 all goose survey: December 8 - 12, 1986

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  17. Fitness-for-duty program at Arkansas nuclear one

    International Nuclear Information System (INIS)

    The key events at Arkansas Power and Light Co. (AP and L) the fitness for duty (FFD) program since January 1981 are outlined. The sequential interface and impact of the US Nuclear Regulatory Commission (NRC) ruling, of the Edison Electric Institute (EEI)-published guidelines, and of the NUMARC commitment have played a key role in the continuing evolution of the FFD program at Arkansas Nuclear One (ANO). The major elements of the FFD program, difficulties encountered, and successful results are described

  18. 10 CFR 71.71 - Normal conditions of transport.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Normal conditions of transport. 71.71 Section 71.71 Energy..., Special Form, and LSA-III Tests 2 § 71.71 Normal conditions of transport. (a) Evaluation. Evaluation of each package design under normal conditions of transport must include a determination of the effect...

  19. 78 FR 48716 - Notice of Availability of the Final Environmental Impact Statement for the Arkansas Valley...

    Science.gov (United States)

    2013-08-09

    ... Arkansas River Basin, groundwater, climate change, recreation biological resources, human environment... Valley Conduit and Long-Term Excess Capacity Master Contract, Fryingpan-Arkansas Project; Bent, Chaffee... statement (EIS) for the Arkansas Valley Conduit (AVC) and Long-Term Excess Capacity Contract,...

  20. Arkansas Superintendents Predict Curriculum for the Year 2000.

    Science.gov (United States)

    Vaupel, Carl F., Jr.; Sweat, Joseph P.

    1985-01-01

    Superintendents of 164 Arkansas small school districts predicted the importance of 20 selected academic courses/physical activities by the year 2000. Courses/activities ranked highest for 2000 were computer science, physics, soccer, economics, and composition. Study assumes collective wisdom or bias of superintendents will alter future secondary…

  1. Arkansas Department of Education Technology Plan, 2008-2012

    Science.gov (United States)

    Arkansas Department of Education, 2008

    2008-01-01

    The Arkansas Department of Education Technology Plan provides policy makers, school districts, education service cooperatives and institutes of higher education with a blueprint that guides and facilitates future state and local technology planning, funding, implementation, and evaluation. The 2008 Plan builds on the progress of the last five-year…

  2. Arkansas and the Southern Regional Education Board, December 2014

    Science.gov (United States)

    Southern Regional Education Board (SREB), 2014

    2014-01-01

    This report details Arkansas's participation in SREB programs and services from December 2013 through November 2014. Appropriations from member states support SREB's core operations and general services. SREB leverages the long-standing commitment of member states to attract external funding for an array of targeted projects for educational…

  3. Recidivism among Arkansas Boot Camp Graduates after 12 Months.

    Science.gov (United States)

    Toombs, Nancy J.; Benda, Brent B.; Corwyn, Robert Flynn

    1997-01-01

    Examines recidivism among 792 graduates of Arkansas' only boot camp. Results indicate that the primary predictors of recidivism were the type of offenses (drugs vs. others), race, and infractions while in boot camp. However, none of the predictors accounted for more than minor proportions of the variance in recidivism. (RJM)

  4. State Minimum Core Curricula: Arkansas Institutions of Higher Education, 2003.

    Science.gov (United States)

    Arkansas State Dept. of Higher Education, Little Rock.

    In 1990 the Arkansas State Board of Higher Education adopted guidelines for the development of state minimum core curriculum response to state legislation. This legislation provides that courses within the core shall apply to the general education core curriculum requirements for baccalaureate degrees at state-supported institutions and shall be…

  5. The Arkansas Autism Developmental Disabilities Monitoring (AR ADDM) project: statewide autism surveillance in a rural state.

    Science.gov (United States)

    Lopez, Maya; Schulz, Eldon G; Baroud, Thaer; Hudson, Allison; Wilson, Mark

    2012-03-01

    In 2002, the Arkansas Autism and Developmental Disabilities Monitoring (AR ADDM) project collected data on the number and characteristics of resident children aged 8 years using a retrospective record review standardized methodology. This paper provides a first-look epidemiology of ASDs among 8 year old Arkansas children using data from the 2002 study year. Overall prevalence estimates, demographic distribution and a temporal lag from concerns identified to diagnosis of ASDs among 8 year olds in Arkansas were similar to that in other sites. Dissemination of information that promotes timely resolution of developmental concerns and improving educational services will benefit children with autism in Arkansas. PMID:22479981

  6. Middle Claiborne Aquifer: Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Outcrop and subcrop extent of the Middle Claiborne Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee.

  7. Middle Claiborne Aquifer: Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Claiborne Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The...

  8. UPPER BUFFALO WILDERNESS AND BUFFALO ADDITION ROADLESS AREA, ARKANSAS.

    Science.gov (United States)

    Miller, Mary H.; Armstrong, Michelle K.

    1984-01-01

    The Upper Buffalo Wilderness and Buffalo Addition Roadless Area covers about 19 sq mi in the Ozark National Forest, Newton County, Arkansas. No metal-bearing minerals were observed during geologic mapping, and analyses for zinc and lead contents in surface rock and sediment samples from the study area are not anomalous. Exploratory drilling into the Boone Formation and (or) the Everton Formation will be necessary to evaluate the mineral-resource potential of zinc and lead in the study area.

  9. Submarine-fan sedimentation, Ouachita Mountains, Arkansas and Oklahoma

    Energy Technology Data Exchange (ETDEWEB)

    Moiola, R.J.; Shanmugam, G.

    1984-09-01

    More than 10,000 m (32,808 ft) of interbedded sandstones and shales comprise the Upper Mississippian and Lower Pennsylvanian flysch succession (Stanley, Jackfork, Johns Valley, Atoka) in the Ouachita Mountains of Arkansas and Oklahoma. Deposited primarily by turbidity current and hemipelagic processes in bathyal and abyssal water depths, these strata formed major submarine-fan complexes that prograded in a westward direction along the axis of an elongate remnant ocean basin that was associated with the collision and suturing of the North American and African-South American plates. A longitudinal fan system is visualized as the depositional framework for these strata, which were deposited in a setting analogous to the modern Bengal fan of the Indian Ocean. Facies analysis of the Jackfork formation indicates that inner fan deposits are present in the vicinity of Little Rock, Arkansas; middle fan channel and interchannel deposits occur at DeGray Dam and Friendship, Arkansas; and outer fan depositional-lobe deposits are present in southeastern Oklahoma. Boulder-bearing units (olistostromes), many with exotic clasts, were shed laterally into the Ouachita basin. They occur throughout the flysch succession and in all fan environments (i.e., inner, middle, and outer). This relationship may serve as a useful criterion for recognizing analogous longitudinal fan systems in the rock record.

  10. WORK SKILL NEEDS FOR THE ECONOMIC DEVELOPMENT OF ARKANSAS, A REPORT.

    Science.gov (United States)

    DAVIS, J. CLARK; AND OTHERS

    A GROWING POPULATION, MIGRATION FROM RURAL TO URBAN AREAS, AND AN EXPANDING MANUFACTURING INDUSTRY IS FORCING ARKANSAS TO REAPPRAISE ITS BASIC SKILLS OFFERINGS IN VOCATIONAL EDUCATION. SINCE ARKANSAS IS NOT IN A POSITION TO PROVIDE MASSIVE INCREASES IN SPENDING FOR VOCATIONAL EDUCATION, THE STATE MUST DECIDE IF IT IS DIRECTING ITS EXPENDITURES TO…

  11. "McLean v. Arkansas" (1982) and Beyond: Implications for Biology Professors

    Science.gov (United States)

    Bland, Mark W.; Moore, Randy

    2011-01-01

    To assess current trends of evolution instruction in high schools of the mid-South, we invited Arkansas high school biology teachers from across the state to respond to a survey designed to address this issue. We also asked students enrolled in a freshman-level, nonmajors biology course at a midsize public Arkansas university to recall their…

  12. Access to and Use of Eye Care Services in Rural Arkansas

    Science.gov (United States)

    Kilmer, Greta; Bynum, LaTonya; Balamurugan, Appathurai

    2010-01-01

    Context: Rural residents are more likely to be uninsured and have low income. Purpose: To determine if rural residents in Arkansas have decreased access to eye care services and use them less frequently than urban residents. Methods: Data from the 2006 Visual Impairment and Access to Eye Care Module from the Arkansas Behavioral Risk Factor…

  13. Association of School-Based Influenza Vaccination Clinics and School Absenteeism--Arkansas, 2012-2013

    Science.gov (United States)

    Gicquelais, Rachel E.; Safi, Haytham; Butler, Sandra; Smith, Nathaniel; Haselow, Dirk T.

    2016-01-01

    Background: Influenza is a major cause of seasonal viral respiratory illness among school-aged children. Accordingly, the Arkansas Department of Health (ADH) coordinates >800 school-based influenza immunization clinics before each influenza season. We quantified the relationship between student influenza vaccination in Arkansas public schools…

  14. Children in Poverty: A Citizen's Guide. A Special Report by Arkansas Kids Count.

    Science.gov (United States)

    Huddleston, Rich

    Noting that child poverty has been a persistent problem for Arkansas children despite improvements in the economy, this Kids Count special report focuses on child poverty in Arkansas. The report examines how child poverty is measured and notes the limitation of existing poverty data; it also discusses the causes and consequences of poverty and…

  15. 77 FR 47779 - Arkansas: Final Authorization of State Hazardous Waste Management Program Revision

    Science.gov (United States)

    2012-08-10

    ... FR 31756-31769 Arkansas Code of 1987 Annotated (A.C.A.) Treatment Sludges from Auto Sections 8-7-201... AGENCY 40 CFR Part 271 Arkansas: Final Authorization of State Hazardous Waste Management Program Revision... to the EPA for Final authorization of the changes to its hazardous waste program under the...

  16. 77 FR 55430 - Arkansas Regulatory Program and Abandoned Mine Land Reclamation Plan

    Science.gov (United States)

    2012-09-10

    ... Access Discharges; Hydrologic Balance: Protection of Groundwater Recharge Capacity; Hydrologic Balance... Environmental Quality, 5301 Northshore Drive, North Little Rock, Arkansas 72118-5317, Telephone: (501) 682-0744... November 21, 1980, Federal Register (45 FR 77003). You can find later actions on the Arkansas program at...

  17. 76 FR 76104 - Arkansas Regulatory Program and Abandoned Mine Land Reclamation Plan

    Science.gov (United States)

    2011-12-06

    ...; Hydrologic Balance: Protection of Groundwater Recharge Capacity; Hydrologic Balance: Surface And Groundwater... Department of Environmental Quality, 5301 Northshore Drive, North Little Rock, Arkansas 72118-5317. Telephone... November 21, 1980, Federal Register (45 FR 77003). You can find later actions on the Arkansas program at...

  18. Review of enterovirus 71 vaccines.

    Science.gov (United States)

    Chong, Pele; Liu, Chia-Chyi; Chow, Yen-Hung; Chou, Ai-Hsiang; Klein, Michel

    2015-03-01

    Enterovirus 71 (EV71) and coxsackieviruses are the major causative agents of hand, foot, and mouth disease (HFMD) outbreaks worldwide and have a significant socioeconomic impact, particularly in Asia. Formalin-inactivated (FI) EV71 vaccines evaluated in human clinical trials in China, Taiwan, and Singapore were found to be safe and to elicit strong neutralizing antibody responses against EV71 currently circulating in Asia. The results from 3 different phase 3 clinical trials performed in young children (6-60 months) indicate that the efficacy of FI-EV71 vaccines is >90% against EV71-related HFMDs and >80% against EV71-associated serious diseases, but the vaccines did not protect against coxsackievirus A16 infections. Here we discuss the critical factors affecting EV71 vaccine product registration, including clinical epidemiology, antigenic shift issues in cross-protection and vaccine strain selection, standardized animal models for potency testing, and cost-effective manufacturing processes for potential incorporation of FI-EV71 vaccine into Expanded Programme on Immunization vaccines. PMID:25352588

  19. Status of Water Levels in Aquifers in the Nacatoch Sand of Southwestern and Northeastern Arkansas and the Tokio Formation of Southwestern Arkansas, February 2005

    Science.gov (United States)

    Schrader, T.P.

    2007-01-01

    The Nacatoch Sand and Tokio Formation aquifers in southwestern Arkansas and the Nacatoch Sand aquifer in northeastern Arkansas are sources of water for industrial, public supply, domestic, and agricultural uses. Potentiometric-surface maps were constructed from water-level measurements made in 60 wells completed in the Nacatoch Sand and 50 wells completed in the Tokio Formation during February 2005. Aquifers in the Nacatoch Sand and Tokio Formation are hereafter referred to as the Nacatoch aquifer and Tokio aquifer, respectively. The direction of ground-water flow in the Nacatoch aquifer in northeastern Arkansas generally is towards the southeast. The potentiometric high is located along the north and northwestern boundaries of the area. The direction of ground-water flow in the Nacatoch aquifer in southwestern Arkansas is towards the south-southeast in Little River, Miller, and Hempstead Counties and to the east-southeast in Nevada and Clark Counties. The potentiometric high is located within the outcrop area in north-central Hempstead County. A cone of depression exists in the Nacatoch aquifer at Hope in southeastern Hempstead County. The direction of ground-water flow in the Tokio aquifer in southwestern Arkansas generally is towards the south or southeast. The potentiometric high is within the outcrop area. An area of artesian flow exists in southeastern Pike, northeastern Hempstead, and northwestern Nevada Counties. One apparent cone of depression might exist northwest of Hope in Hempstead County. In northeastern Arkansas, withdrawals from the Nacatoch aquifer increased by 516 percent from 1965 to 2000. In southwestern Arkansas, withdrawals from Nacatoch aquifer and Tokio aquifer increased by 125 percent and 201 percent, respectively, from 1965 to 1980 and decreased by 93 percent and 80 percent, respectively, from 1980 to 2000. Long-term hydrographs were prepared for 10 wells in the study areas. Changes in water levels in some wells may be associated with

  20. Status of Water Levels in Aquifers in the Nacatoch Sand of Southwestern and Northeastern Arkansas and the Tokio Formation of Southwestern Arkansas, 1999

    Science.gov (United States)

    Schrader, Tony P.

    1999-01-01

    Nacatoch Sand and Tokio Formation aquifers in southwestern Arkansas and the Nacatoch Sand aquifer in northeastern Arkansas are a source of water for industrial, public supply, domestic, and agricultural uses. Potentiometric-surface maps were constructed from water-level measurements made in 59 wells completed in the Nacatoch Sand and 45 wells completed in the Tokio Formation from April through May 1999. The direction of ground-water flow in the aquifer in the Nacatoch Sand in northeastern Arkansas generally is towards the southeast. The potentiometric high is located along the north and northwestern boundaries. The direction of ground-water flow in the aquifer in the Nacatoch Sand in southwestern Arkansas is towards the south-southeast in Little River, Miller, and Hempstead Counties and to the east-southeast in Nevada and Clark Counties. The potentiometric high is located within the outcrop area in north-central Hempstead County. A cone of depression exists in the aquifer in the Nacatoch Sand at Hope in southeastern Hempstead County. The direction of ground-water flow in the aquifer in the Yokio Formation in southwestern Arkansas generally is towards the southeast. The potentiometric high is within the outcrop area. An area of artesian flow exists in southeastern Pike, northeastern Hempstead, and northwestern Nevada Counties. One apparent cone of depression might exist northwest of Hope in Hempstead County. In northeastern Arkansas, withdrawals from the Nacatoch Sand increased by 736 percent from 1965 to 1995. In southwestern Arkansas withdrawals from aquifers in the Nacatoch Sand and Yokio Formation increased by 125 percent and 201 percent, respectively, from 1965 to 1980 and decreased by 78 percent and 63 percent, respectively, from 1980 to 1995. Long-term hydrographs were prepared for 13 wells in the study area. Changes in water levels in some wells may be associated with changes in withdrawals from the respective aquifers.

  1. First isolation of enterovirus 71 (EV-71 from Northern Brazil

    Directory of Open Access Journals (Sweden)

    Lamarão Leticia Martins

    2003-01-01

    Full Text Available Enterovirus 71 (EV-71 has been associated to cases of neurological disease in many countries including Brazil. This virus has now been reported from three of the five Brazilian regions. Our study relates the findings concerning to the first isolate of this virus in Northern region of Brazil. A 15-month old female patient, from the rural zone of the municipality of Santana do Araguaia in southern Pará state was admitted at the hospital with acute, flaccid, asymmetric and ascending motor deficiency, located in the right lower limb. Stools samples from this child were inoculated in RD cells and was isolated an EV-71. We plan to sequence our strain and compare it to other isolates in Brazil. Differences at the molecular level can explain why EV-71 strains circulating in other continents, such as Asia, appear to be more virulent.

  2. Arkansas State Briefing Book for low-level radioactive waste management

    International Nuclear Information System (INIS)

    The Arkansas State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. This report contains a profile of low-level radioactive waste generators in Arkansas. The profile is the result of a survey of NRC licensees in Arkansas. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Arkansas

  3. Mississippi River Valley Alluvial Aquifer, Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, Tennessee; 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Outcrop and subcrop extent of the Mississippi River Valley Alluvial Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, and Tennessee.

  4. Upper Claiborne Aquifer: Alabama, Arkansas, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Upper Claiborne Aquifer in Arkansas, Alabama, Louisiana, Missouri, Mississippi, and Tennessee. The hydrogeologic unit dataset...

  5. Middle Claiborne Confining Unit: Alabama, Arkansas, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Claiborne Confining Unit in Alabama, Arkansas, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The hydrogeologic...

  6. An Evaluation of Ecosystem Restoration Options for Grand Prairie Region of Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Evaluation of Ecosystem Restoration and Management Options covers the hydrogeomorphic analysis HGM for Grand Prairie Region of Arkansas. This three step...

  7. Middle Wilcox Aquifer: Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Wolcox Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The hydrogeologic...

  8. Arkansas State Briefing Book for low-level radioactive-waste management

    International Nuclear Information System (INIS)

    The Arkansas State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Arkansas. The profile is the result of a survey of NRC licensees in Arkansas. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Arkansas

  9. Lower Wilcox Aquifer: Alabama, Arkansas, Illinois, Kentucky, Missouri, Mississippi 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the in Alabama, Arkansas, Illinois, Kentucky, Missouri, Mississippi. The hydrogeologic unit dataset contains 414 rows and 394...

  10. Annual mid-December goose survey - December 14 - 18, 1981: Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, and Snow Geese are included.

  11. Employment Discrimination Based on Sexual Orientation and Gender Identity in Arkansas

    OpenAIRE

    Mallory, Christy; Sears, Brad

    2015-01-01

    None of the approximately 47,000 LGBT workers in Arkansas are explicitly protected from discrimination under local, state or federal laws. If sexual orientation and gender identity were added to existing statewide non-discrimination laws, 22 more complaints would be filed in Arkansas each year. The cost of enforcing those complaints would be negligible, and would not require additional court or administrative staff.

  12. Status of Water Levels and Selected Water-Quality Conditions in the Mississippi River Valley Alluvial Aquifer in Eastern Arkansas, 2004

    Science.gov (United States)

    Schrader, T.P.

    2006-01-01

    analysis of long-term water-level changes (1980-2004) in the depression in Arkansas and Prairie Counties shows the effects of the elongation of this depression. Water samples were collected from 138 wells completed in the alluvial aquifer and measured onsite for specific conductance and temperature. Samples were collected at 71 wells for dissolved chloride analysis at the U.S. Geological Survey National Water Quality Laboratory. Specific conductance ranged from 205 microsiemens per centimeter at 25 degrees Celsius at a well in Lonoke County to 1,440 microsiemens per centimeter at 25 degrees Celsius at a well in Monroe County.

  13. Structure determination of enterovirus 71

    Energy Technology Data Exchange (ETDEWEB)

    Plevka, Pavel; Perera, Rushika; Cardosa, Jane; Kuhn, Richard J.; Rossmann, Michael G. (Purdue); (Sentinext)

    2013-02-20

    Enterovirus 71 is a picornavirus that causes hand, foot and mouth disease but may induce fatal neurological illness in infants and young children. Enterovirus 71 crystallized in a body-centered orthorhombic space group with two particles in general orientations in the crystallographic asymmetric unit. Determination of the particle orientations required that the locked rotation function excluded the twofold symmetry axes from the set of icosahedral symmetry operators. This avoided the occurrence of misleading high rotation-function values produced by the alignment of icosahedral and crystallographic twofold axes. Once the orientations and positions of the particles had been established, the structure was solved by molecular replacement and phase extension.

  14. Ooid mineralogy and diagenesis of upper Mississippian Pitkin Formation, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Heydari, E.; Snelling, R.D.; Dawson, W.C.; Machain, M.L. (Louisiana State Univ., Baton Rouge (USA))

    1989-08-01

    The Pitkin formation is a marine oolitic-bioclastic limestone exhibiting shoal, lagoonal, beach, and tidal-channel facies. Ooids formed on the Pitkin carbonate shelf show lateral and vertical variations in original mineralogy. In a section south of Mountain View, Arkansas, the lower marine shoal and shoreface facies are composed of originally aragonitic ooids. These ooids are replaced by neomorphic calcite and exhibit elevated strontium and low magnesium concentrations. The overlying lagoonal facies are composed of originally calcite ooids that have retained their original radial fabrics. These ooids exhibit low strontium and high magnesium concentration. Ooids in sections to the west of Mountain View are all calcitic. Early diagenesis of the formation is dominated by marine cementation. Postdepositional diagenesis encompasses extensive dissolution of originally aragonite and magnesian calcite components, precipitation of a late unzoned ferroan calcite, and minor amounts of saddle dolomite. Grainstone units lack any intergranular porosity. Pressure solution contacts among grains in grainstones are generally uncommon. Cementation alone is responsible for destruction of all of the original porosity in these rocks. Intensive pressure solution is observed only in packstone units not affected by early marine cementation.

  15. Human enterovirus 71 epidemics: what's next?

    OpenAIRE

    Yip, Cyril C. Y.; Lau, Susanna K. P.; Woo, Patrick C. Y.; Yuen, Kwok-Yung

    2013-01-01

    Human enterovirus 71 (EV71) epidemics have affected various countries in the past 40 years. EV71 commonly causes hand, foot and mouth disease (HFMD) in children, but can result in neurological and cardiorespiratory complications in severe cases. Genotypic changes of EV71 have been observed in different places over time, with the emergence of novel genotypes or subgenotypes giving rise to serious outbreaks. Since the late 1990s, intra- and inter-typic recombination events in EV71 have been inc...

  16. Rural Industralization and Population Growth: The Case of Arkansas. ORNL-HUD-4, Civil Defense Research Project.

    Science.gov (United States)

    Stuart, Alfred W.

    Utilizing available 1966 statistical data and U.S. Census figures, the State of Arkansas was examined for purposes of analyzing: (1) the extent to which recent population gains in states such as Arkansas and Oklahoma is occurring in rural areas rather than as an extension of urbanization; (2) the extent to which U.S. manufacturing is beginning to…

  17. Sadness, suicide, and bullying in Arkansas: results from the Youth Risk Behavior Survey -- 2011.

    Science.gov (United States)

    Kindrick, Kristi; Castro, Juan; Messias, Erick

    2013-10-01

    Bullying is a common exposure in high school and more recently cyberbullying has become prevalent among teens. We used the 2011 Arkansas Youth Risk Behavior Survey to estimate the prevalence of school bullying and cyberbullying and to measure its association with teen suicidality. In Arkansas, 11.6% of students reported only school bullying, 6.2% only cyberbullying, and 10.2% both forms of bullying. We determined "feeling unsafe at school" was a significant risk factor for depression and all suicide questions. We also found that being a victim of school bullying, cyberbullying, or both, increased the risk for depression, suicidal ideation, and plan. PMID:24383197

  18. Strategies to develop antivirals against enterovirus 71

    OpenAIRE

    Kuo, Rei-Lin; Shih, Shin-Ru

    2013-01-01

    Enterovirus 71 (EV71) is an important human pathogen which may cause severe neurological complications and death in children. The virus caused several outbreaks in the Asia-Pacific region during the past two decades and has been considered a significant public health problem in the post-poliovirus eradication era. Unlike poliovirus, there is no effective vaccine or approved antivirals against EV71. To explore anti-EV71 agents therefore is of vital importance. Several strategies have been empl...

  19. 16 CFR 1.71 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Administration. 1.71 Section 1.71 Commercial Practices FEDERAL TRADE COMMISSION ORGANIZATION, PROCEDURES AND RULES OF PRACTICE GENERAL PROCEDURES Administration of the Fair Credit Reporting Act § 1.71 Administration. The general administration of the...

  20. 7 CFR 985.71 - Effective time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Effective time. 985.71 Section 985.71 Agriculture....71 Effective time. The provisions of this subpart, and of any amendment thereto, shall become effective at such time as the Secretary may declare and shall continue in force until terminated...

  1. 7 CFR 1412.71 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Administration. 1412.71 Section 1412.71 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF...) Program § 1412.71 Administration. (a) All of the provisions of this part apply to this subpart. To...

  2. 7 CFR 7.1 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Administration. 7.1 Section 7.1 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.1 Administration. (a) The regulations of this part...

  3. 10 CFR 39.71 - Security.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Security. 39.71 Section 39.71 Energy NUCLEAR REGULATORY COMMISSION LICENSES AND RADIATION SAFETY REQUIREMENTS FOR WELL LOGGING Security, Records, Notifications § 39.71 Security. (a) A logging supervisor must be physically present at a temporary jobsite...

  4. 28 CFR 71.25 - Fees.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Fees. 71.25 Section 71.25 Judicial... REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71.25 Fees. The party requesting a subpoena shall pay the cost of the fees and mileage of any witness subpoenaed in...

  5. 10 CFR 34.71 - Utilization logs.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Utilization logs. 34.71 Section 34.71 Energy NUCLEAR... RADIOGRAPHIC OPERATIONS Recordkeeping Requirements § 34.71 Utilization logs. (a) Each licensee shall maintain utilization logs showing for each sealed source the following information: (1) A description, including...

  6. 40 CFR 233.71 - New Jersey.

    Science.gov (United States)

    2010-07-01

    ... Director of the Federal Register in accordance with 552(a) and 1 CFR part 51. Material is incorporated as... 40 Protection of Environment 24 2010-07-01 2010-07-01 false New Jersey. 233.71 Section 233.71... REGULATIONS Approved State Programs § 233.71 New Jersey. The applicable regulatory program for discharges...

  7. 22 CFR 71.12 - Dietary supplements.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Dietary supplements. 71.12 Section 71.12... Incarcerated Abroad § 71.12 Dietary supplements. (a) Eligibility criteria. A prisoner is considered eligible for the dietary supplement program under the following general criteria: (1) An evaluation by...

  8. 45 CFR 1321.71 - Legal assistance.

    Science.gov (United States)

    2010-10-01

    ... speak English as their principal language. (d) A legal assistance provider may not require an older... 45 Public Welfare 4 2010-10-01 2010-10-01 false Legal assistance. 1321.71 Section 1321.71 Public... AND COMMUNITY PROGRAMS ON AGING Service Requirements § 1321.71 Legal assistance. (a) The...

  9. 10 CFR 71.37 - Quality assurance.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance. 71.37 Section 71.37 Energy NUCLEAR... Package Approval § 71.37 Quality assurance. (a) The applicant shall describe the quality assurance program... quality assurance program that are applicable to the particular package design under...

  10. 10 CFR 820.71 - Standard.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Standard. 820.71 Section 820.71 Energy DEPARTMENT OF ENERGY PROCEDURAL RULES FOR DOE NUCLEAR ACTIVITIES Criminal Penalties § 820.71 Standard. If a person subject to the Act or the DOE Nuclear Safety Requirements has, by act or omission, knowingly and...

  11. 40 CFR 68.71 - Training.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Training. 68.71 Section 68.71... ACCIDENT PREVENTION PROVISIONS Program 3 Prevention Program § 68.71 Training. (a) Initial training. (1... operating a newly assigned process, shall be trained in an overview of the process and in the...

  12. 29 CFR 71.15 - Training.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Training. 71.15 Section 71.15 Labor Office of the Secretary... § 71.15 Training. All DOL systems managers, disclosure officers, and employees with responsibilities under the Privacy Act shall periodically attend training offered by the Department on the Privacy Act....

  13. 50 CFR 648.71 - Catch quotas.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Catch quotas. 648.71 Section 648.71 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION... Clam and Ocean Quahog Fisheries § 648.71 Catch quotas. (a) Establishing quotas. Beginning in 2005,...

  14. 22 CFR 41.71 - Transit aliens.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is...

  15. 50 CFR 25.71 - Public safety.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public safety. 25.71 Section 25.71... NATIONAL WILDLIFE REFUGE SYSTEM ADMINISTRATIVE PROVISIONS Safety Regulations § 25.71 Public safety. Persons using national wildlife refuges shall comply with the safety requirements which are established...

  16. 49 CFR 71.8 - Mountain zone.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Mountain zone. 71.8 Section 71.8 Transportation Office of the Secretary of Transportation STANDARD TIME ZONE BOUNDARIES § 71.8 Mountain zone. The fourth zone, the mountain standard time zone, includes that part of the United States that is west of...

  17. 40 CFR 71.5 - Permit applications.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Permit applications. 71.5 Section 71.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL... schedule established pursuant to § 71.9. (1) Identifying information, including company name and...

  18. 21 CFR 1316.71 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... Substances Act (84 Stat. 1242; 21 U.S.C. 801) and/or the Controlled Substances Import and Export Act (84 Stat... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Definitions. 1316.71 Section 1316.71 Food and... PROCEDURES Seizure, Forfeiture, and Disposition of Property § 1316.71 Definitions. As used in this...

  19. 46 CFR 71.65-1 - General.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS INSPECTION AND CERTIFICATION Plan Approval § 71.65-1 General. (a) The list of required plans is general in character, but includes all plans in § 71... arrangement, construction and required equipment. (b) In the list of required plans in § 71.65-5 the...

  20. 10 CFR 30.71 - Schedule B.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Schedule B. 30.71 Section 30.71 Energy NUCLEAR REGULATORY COMMISSION RULES OF GENERAL APPLICABILITY TO DOMESTIC LICENSING OF BYPRODUCT MATERIAL Schedules § 30.71 Schedule B. Byproduct material Microcuries Antimony 122 (Sb 122) 100 Antimony 124 (Sb 124) 10 Antimony...

  1. 10 CFR 71.95 - Reports.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Reports. 71.95 Section 71.95 Energy NUCLEAR REGULATORY... § 71.95 Reports. (a) The licensee, after requesting the certificate holder's input, shall submit a written report to the Commission of— (1) Instances in which there is a significant reduction in...

  2. 10 CFR 71.123 - Test control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a test program to assure that all testing required...

  3. 14 CFR 437.71 - Flight rules.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Flight rules. 437.71 Section 437.71... TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.71 Flight rules. (a) Before initiating rocket-powered flight, a permittee must confirm that all systems and operations necessary to ensure...

  4. 7 CFR 905.71 - Other information.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Other information. 905.71 Section 905.71 Agriculture... TANGELOS GROWN IN FLORIDA Order Regulating Handling Handlers' Reports § 905.71 Other information. Upon... committee, in such manner and at such times as it prescribes, such other information as will enable it...

  5. 21 CFR 201.71 - Magnesium labeling.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 4 2010-04-01 2010-04-01 false Magnesium labeling. 201.71 Section 201.71 Food and... LABELING Labeling Requirements for Over-the-Counter Drugs § 201.71 Magnesium labeling. (a) The labeling of over-the-counter (OTC) drug products intended for oral ingestion shall contain the magnesium...

  6. 27 CFR 71.83 - Evidence.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Evidence. 71.83 Section 71... General § 71.83 Evidence. Any evidence which would be admissible under the rules of evidence governing... his case or defense by oral or documentary evidence, depositions, duly authenticated copies of...

  7. 28 CFR 71.34 - Evidence.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Evidence. 71.34 Section 71.34 Judicial... REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71.34 Evidence. (a) The ALJ shall determine the admissibility of evidence. (b) Except as provided in this part, the...

  8. 14 CFR 27.71 - Autorotation performance.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Autorotation performance. 27.71 Section 27.71 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: NORMAL CATEGORY ROTORCRAFT Flight Performance § 27.71 Autorotation performance....

  9. 30 CFR 71.702 - Asbestos standard.

    Science.gov (United States)

    2010-07-01

    ...) using the OSHA Reference Method in OSHA's asbestos standard found in 29 CFR 1910.1001, Appendix A, or a... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Asbestos standard. 71.702 Section 71.702... Contaminants § 71.702 Asbestos standard. (a) Definitions. Asbestos is a generic term for a number...

  10. 27 CFR 71.36 - General.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false General. 71.36 Section 71.36 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE... Settlement Informal Settlement § 71.36 General. In all proceedings in which a permittee is cited to...

  11. 27 CFR 13.71 - Informal conferences.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Informal conferences. 13.71 Section 13.71 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS LABELING PROCEEDINGS Miscellaneous § 13.71 Informal conferences....

  12. Teacher Pension Incentives, Retirement Behavior, and Potential for Reform in Arkansas. Conference Paper 2009-10

    Science.gov (United States)

    Costrell, Robert M.; McGee, Josh B.

    2009-01-01

    In this paper, we present an analysis of the Arkansas Teacher Retirement System (ATRS) pension plan and an empirical investigation of the behavioral response to that plan, as well as to a possible reform plan. We begin by describing the plan parameters and discussing the incentives these parameters create. We then estimate the effect of pension…

  13. Teacher Pension Incentives, Retirement Behavior, and Potential for Reform in Arkansas

    Science.gov (United States)

    Costrell, Robert M.; McGee, Josh B.

    2010-01-01

    The authors analyze the Arkansas teacher pension plan and empirically gauge the behavioral response to incentives embedded in that plan and to possible reforms. The pattern of pension wealth accrual creates sharp incentives to work until eligible for early or normal retirement, often in one's early fifties, and to separate shortly thereafter. We…

  14. Drought and deluge: Effects of recent climate variability on groundwater levels in eastern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Schrader, T.P.

    2013-01-01

    Arkansas experienced wide extremes in climate variability during the period of 2005 to 2010, recording the largest annual precipitation ever recorded in the State (100.05 inches) in 2009. Many weather stations across the State reported between 80 to 90 inches of rainfall in 2009. For comparison, the average annual precipitation in Little Rock, Arkansas, for the period 1878 to 2010 was 47.1 inches. In contrast, 2005 and 2010 were the 7th and 14th driest years on record in Little Rock with 34.55 and 36.52 inches, respectively; both tied as the hottest years ever recorded in Arkansas. The wettest year on record in Little Rock (2009) was interspersed within these dry years, with a total of 81.79 inches. Fifteen weather stations within the State ranked 2009 as the wettest year on record. Extremes in annual precipitation rates may lead to greater variability in groundwater recharge rates and water use, particularly in the agricultural areas in eastern Arkansas that rely heavily on groundwater produced from the Mississippi River Valley alluvial aquifer (hereafter referred to as the alluvial aquifer). How does this variability affect the groundwater system and water use therein? Are the effects of this variability discernable in measured water levels in wells? Czarnecki and Schrader examined these questions and provided some insights, the results of which are presented here.

  15. Impact of Spina Bifida on Parental Caregivers: Findings from a Survey of Arkansas Families

    Science.gov (United States)

    Grosse, Scott D.; Flores, Alina L.; Ouyang, Lijing; Robbins, James M.; Tilford, John M.

    2009-01-01

    The well-being of caregivers of children with spina bifida and other conditions is an important topic. We interviewed the primary caregivers of 98 children aged 0-17 years with spina bifida sampled from a population-based birth defects registry in Arkansas and the caregivers of 49 unaffected children. Measures of caregiver well-being were compared…

  16. 77 FR 52365 - Notice of Availability of the Draft Environmental Impact Statement for the Arkansas Valley...

    Science.gov (United States)

    2012-08-29

    ... are analyzed in the Draft EIS: (1) Construct and operate the Arkansas Valley Conduit (AVC) and enter... and delivery purposes, the proposed actions are independent of each other. The AVC was authorized by... annual federal funding as necessary for constructing AVC, and included a cost sharing plan with...

  17. Field evaluation of four spatial repellent devices against Arkansas rice-land mosquitoes

    Science.gov (United States)

    Four commercially available spatial repellent devices were tested in a rice land habitat near Stuttgart, Arkansas after semi-field level assessments had been made at the Center for Medical, Agricultural, and Veterinary Entomology, ARS, USDA in Gainesville, FL. OFF! Clip-On® (a.i. metofluthrin, S.C....

  18. Pharmaceuticals and other organic chemicals in selected north-central and northwestern Arkansas streams

    Science.gov (United States)

    Haggard, B.E.; Galloway, J.M.; Green, W.R.; Meyer, M.T.

    2006-01-01

    Recently, our attention has focused on the low level detection of many antibiotics, pharmaceuticals, and other organic chemicals in water resources. The limited studies available suggest that urban or rural streams receiving wastewater effluent are more susceptible to contamination. The purpose of this study was to evaluate the occurrence of antibiotics, pharmaceuticals, and other organic chemicals at 18 sites on seven selected streams in Arkansas, USA, during March, April, and August 2004. Water samples were collected upstream and downstream from the influence of effluent discharges in northwestern Arkansas and at one site on a relatively undeveloped stream in north-central Arkansas. At least one antibiotic, pharmaceutical, or other organic chemical was detected at all sites, except at Spavinaw Creek near Mayesville, Arkansas. The greatest number of detections was observed at Mud Creek downstream from an effluent discharge, including 31 pharmaceuticals and other organic chemicals. The detection of these chemicals occurred in higher frequency at sites downstream from effluent discharges compared to those sites upstream from effluent discharges; total chemical concentration was also greater downstream. Wastewater effluent discharge increased the concentrations of detergent metabolites, fire retardants, fragrances and flavors, and steroids in these streams. Antibiotics and associated degradation products were only found at two streams downstream from effluent discharges. Overall, 42 of the 108 chemicals targeted in this study were found in water samples from at least one site, and the most frequently detected organic chemicals included caffeine, phenol, para-cresol, and acetyl hexamethyl tetrahydro naphthalene (AHTN). ?? ASA, CSSA, SSSA.

  19. 76 FR 36142 - Arkansas; Amendment No. 6 to Notice of a Major Disaster Declaration

    Science.gov (United States)

    2011-06-21

    ... Unemployment Assistance (DUA); 97.046, Fire Management Assistance Grant; 97.048, Disaster Housing Assistance to... Assistance--Disaster Housing Operations for Individuals and Households; 97.050, Presidentially Declared... SECURITY Federal Emergency Management Agency Arkansas; Amendment No. 6 to Notice of a Major...

  20. 76 FR 37360 - Arkansas; Amendment No. 7 to Notice of a Major Disaster Declaration

    Science.gov (United States)

    2011-06-27

    ..., Disaster Unemployment Assistance (DUA); 97.046, Fire Management Assistance Grant; 97.048, Disaster Housing... Declared Disaster Assistance--Disaster Housing Operations for Individuals and Households; 97.050... SECURITY Federal Emergency Management Agency Arkansas; Amendment No. 7 to Notice of a Major...

  1. Child Care Needs of Mothers in Arkansas' Labor Force: A Statistical Study.

    Science.gov (United States)

    Arkansas Advocates for Children and Families, Little Rock.

    The major purpose of this paper is to increase employers' awareness of the impact that the rising number of working mothers has had on the need for day care in Arkansas as a whole and in their specific business locales. It is hoped that employers will be motivated to investigate and evaluate the advantages of providing some form of day care…

  2. 77 FR 14603 - Approval and Promulgation of Implementation Plans; Arkansas; Regional Haze State Implementation...

    Science.gov (United States)

    2012-03-12

    ... determinations for the American Electric Power (AEP) Flint Creek Plant Boiler No. 1; the NO X BART determination... and 2; the BART determination for the Entergy White Bluff Plant Auxiliary Boiler; the SO 2 and NO X... from Arkansas's RH SIP: The PM BART determination for the AEP Flint Creek Plant Boiler No. 1. The SO...

  3. Creating a Caring Community: The University of Arkansas Nursery School Experience.

    Science.gov (United States)

    Baldwin, Vernoice G.; DaRos-Voseles, Denise A.; Swick, Kevin J.

    2003-01-01

    This article describes seven dimensions of establishing a caring community at the University of Arkansas Nursery School in Fayetteville. Key components relate to the director setting the tone for the school, the instructor-student relationship, empathetic teacher-child interactions, empathetic teacher-parent interactions, celebration of…

  4. Risks, Assets, and Negative Health Behaviors among Arkansas' Hispanic Adolescents

    Science.gov (United States)

    Fitzpatrick, Kevin M.; Choudary, Wendie; Kearney, Anne; Piko, Bettina F.

    2013-01-01

    This study examined the relationship between risk, assets, and negative health behaviors among a large sample of Hispanic adolescents. Data were collected from over 1,000 Hispanic youth in grades 6, 8, 10, and 12 attending school in a moderate size school district in Northwest Arkansas. Logistic regression models examined the variation in the odds…

  5. Unpublished Interim Digital Geologic Map of Hot Springs National Park and Vicinity, Arkansas (NPS, GRD, GRI, HOSP, HOSP digital map) adapted from the interim Arkansas Geological Survey DGM-HSR-003 by Johnson and Hanson (2011)

    Data.gov (United States)

    National Park Service, Department of the Interior — The Unpublished Interim Digital Geologic Map of Hot Springs National Park and Vicinity, Arkansas is composed of GIS data layers complete with ArcMap 9.3 layer...

  6. Milrinone in Enterovirus 71 Brain Stem Encephalitis

    Science.gov (United States)

    Wang, Shih-Min

    2016-01-01

    Enterovirus 71 (EV71) was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD) across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE) is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Milrinone is a type III phosphodiesterase inhibitor that has both inotropic and vasodilator effects. Its clinical efficacy has been shown by modulating inflammation, reducing sympathetic over-activity, and improving survival in patients with EV71-associated PE. Milrinone exhibits immunoregulatory and anti-inflammatory effects in the management of systemic inflammatory responses in severe EV71 infection. PMID:27065870

  7. Milrinone in Enterovirus 71 Brain Stem Encephalitis.

    Science.gov (United States)

    Wang, Shih-Min

    2016-01-01

    Enterovirus 71 (EV71) was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD) across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE) is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Milrinone is a type III phosphodiesterase inhibitor that has both inotropic and vasodilator effects. Its clinical efficacy has been shown by modulating inflammation, reducing sympathetic over-activity, and improving survival in patients with EV71-associated PE. Milrinone exhibits immunoregulatory and anti-inflammatory effects in the management of systemic inflammatory responses in severe EV71 infection. PMID:27065870

  8. Inhibition of Enterovirus 71 (EV-71) Infections by a Novel Antiviral Peptide Derived from EV-71 Capsid Protein VP1

    Science.gov (United States)

    Tan, Chee Wah; Chan, Yoke Fun; Sim, Kooi Mow; Tan, Eng Lee; Poh, Chit Laa

    2012-01-01

    Enterovirus 71 (EV-71) is the main causative agent of hand, foot and mouth disease (HFMD). In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers) overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD) cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC50 values ranging from 6–9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71. PMID:22563456

  9. Inhibition of enterovirus 71 (EV-71 infections by a novel antiviral peptide derived from EV-71 capsid protein VP1.

    Directory of Open Access Journals (Sweden)

    Chee Wah Tan

    Full Text Available Enterovirus 71 (EV-71 is the main causative agent of hand, foot and mouth disease (HFMD. In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC(50 values ranging from 6-9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71.

  10. Diseases caused by enterovirus 71 infection.

    Science.gov (United States)

    Lee, Ta-Chung; Guo, How-Ran; Su, Huey-Jen Jenny; Yang, Yi-Ching; Chang, Hsiao-Ling; Chen, Kow-Tong

    2009-10-01

    The purpose of this review was to explore the epidemiology, pathogenesis, virology, and management of enterovirus 71 (EV71) infection. Published literature was surveyed by Medline using the keyword "EV71." The reported incidence of cases of hand-foot-mouth disease/herpangina varied from year to year; seasonal variations in incidence were observed, with a peak in incidence during the summer season. Most cases of hand-foot-mouth disease/herpangina hospitalized for complications occurred in children less than 5 years old. The brainstem was the most likely major target of EV71 infection. Different enteroviruses cocirculate in the community annually. The emergence of the EV71 epidemic in the Asia Pacific region has been associated with the circulation of 5 genetic lineages (genotypes B3, B4, C1, C2, C4) that appear to be undergoing rapid evolutionary changes. The relationship between the gene structure of the EV71 virus and the factors that ensure its survival, ease of transmission, and evasion of immunity is still unclear. EV71 central nervous system involvement causes serious clinical illness, death, and long-term neurologic and psychiatric disorders in young children. EV71 infection has emerged as an important public health problem. Vaccine development is recommended for the prevention of EV71 infection in the future. PMID:20118685

  11. Development of Novel Vaccines against Enterovirus-71

    OpenAIRE

    Pinn Tsin Isabel Yee; Chit Laa Poh

    2015-01-01

    The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71) and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and tr...

  12. Burnup verification at Arkansas Nuclear One-unit 1 using the Fork measurement system

    International Nuclear Information System (INIS)

    The Fork measurement system, designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program, has been used for several years to examine spent fuel assemblies at nuclear reactors around the world. The objective of the test program described here is to demonstrate the ability of the Fork system to verify the records for assembly burnup at U.S. nuclear utilities. The measurements described here were performed at Arkansas Nuclear One, operated by Energy Operations, Inc. The Fork system was used to examine 34 assemblies in the storage pool of Arkansas Nuclear One-Unit 1. The correlation between the neutron measurements and the reactor records produced an average random deviation in burnup of 3.0% from the calibration, which translates into an average variation of 2.2% in the reactor records for burnup. The system proved to be compatible with utility operations

  13. Reactor building temperature reduction project data acquisition, evaluations and proposed actions. Arkansas nuclear one, Unit 1

    International Nuclear Information System (INIS)

    On August 1, 1987 Arkansas Power and Light maintenance personnel made a power entry at Arkansas Nuclear One, Unit 1. They discovered that the steam generator cavity temperature was too high for personnel comfort. AP and L management requested that the available temperature data be recorded throughout the reactor building (RB). Hand held thermometers, infrared thermography, and existing RTD's were utilized. Temperatures were found to range from 100F near the basement to 165F in the dome area. A temperature of 183F was found above the A steam generator cavity. As a result of this finding, AP and L and the Nuclear Regulatory Commission decided that an in depth evaluation of the RB temperatures and their effect on the safe operation of the plant was needed. The original design temperature was 110F. This section discusses the monitoring systems installed, the results of the evaluation and the corrective actions taken

  14. Building Partnerships With Rural Arkansas Faith Communities to Promote Veterans’ Mental Health: Lessons Learned

    Science.gov (United States)

    Sullivan, Greer; Hunt, Justin; Haynes, Tiffany F.; Bryant, Keneshia; Cheney, Ann M.; Pyne, Jeffrey M.; Reaves, Christina; Sullivan, Steve; Lewis, Caleb; Barnes, Bonita; Barnes, Michael; Hudson, Cliff; Jegley, Susan; Larkin, Bridgette; Russell, Shane; White, Penny; Gilmore, LaNissa; Claypoole, Sterling; Smith, Rev. Johnny; Richison, Ruth

    2014-01-01

    Background The Mental Health–Clergy Partnership Program established partnerships between institutional (Department of Veterans’ Affairs [VA] chaplains, mental health providers) and community (local clergy, parishioners) groups to develop programs to assist rural veterans with mental health needs. Objectives Describe the development, challenges, and lessons learned from the Mental Health–Clergy Partnership Program in three Arkansas towns between 2009 and 2012. Methods Researchers identified three rural Arkansas sites, established local advisory boards, and obtained quantitative ratings of the extent to which partnerships were participatory. Results Partnerships seemed to become more participatory over time. Each site developed distinctive programs with variation in fidelity to original program goals. Challenges included developing trust and maintaining racial diversity in local program leadership. Conclusions Academics can partner with local faith communities to create unique programs that benefit the mental health of returning veterans. Research is needed to determine the effectiveness of community based programs, especially relative to typical “top-down” outreach approaches. PMID:24859098

  15. Cost-effectiveness of the US Geological Survey stream-gaging program in Arkansas

    Science.gov (United States)

    Darling, M.E.; Lamb, T.E.

    1984-01-01

    This report documents the results of the cost-effectiveness of the stream-gaging program in Arkansas. Data uses and funding sources were identified for the daily-discharge stations. All daily-discharge stations were found to be in one or more data use categories, and none were candidates for alternate methods which would result in discontinuation or conversion to a partial record station. The cost for operation of daily-discharge stations and routing costs to partial record stations, crest gages, pollution control stations as well as seven recording ground-water stations was evaluated in the Kalman-Filtering Cost-Effective Resource allocation (K-CERA) analysis. This operation under current practices requires a budget of $292,150. The average standard error of estimate of streamflow record for the Arkansas District was analyzed at 33 percent.

  16. A new species of Sellaphora (Sellaphoraceae) from Hannaberry Lake, Arkansas, U.S.A.

    OpenAIRE

    Enache, Mihaela D.; Potapova, Marina

    2009-01-01

    A new small-size species of Sellaphora was found in sediments from Hannaberry Lake, Arkansas, during the National Lakes Assessment project conducted by the United States Environmental Protection Agency. The species was studied with light microscopy and scanning electron microscopy. It differs from previously reported Sellaphora species by its small and delicate frustule with striation irresolvable in light microscopy. Here we present details on its morphology and size variation and report the...

  17. Fruit and Vegetable Consumption among College Students in Arkansas and Florida: Food Culture vs. Health Knowledge

    OpenAIRE

    Schroeter, Christiane; House, Lisa; Lorence, Argelia

    2007-01-01

    This study determines the impact of demographics, dietary and health knowledge, and food culture on fruit and vegetable consumption of college students in Arkansas and Florida. Our empirical analysis demonstrates that food culture significantly impacts consumption of fruits and vegetables; a finding which emphasizes the need to target cultural aspects when developing effective and efficient management of agribusiness firms. Understanding the antecedents to consumption for products like fruits...

  18. Arkansas and Louisiana Aeromagnetic and Gravity Maps and Data - A Website for Distribution of Data

    Science.gov (United States)

    Bankey, Viki; Daniels, David L.

    2008-01-01

    This report contains digital data, image files, and text files describing data formats for aeromagnetic and gravity data used to compile the State aeromagnetic and gravity maps of Arkansas and Louisiana. The digital files include grids, images, ArcInfo, and Geosoft compatible files. In some of the data folders, ASCII files with the extension 'txt' describe the format and contents of the data files. Read the 'txt' files before using the data files.

  19. Atmospheric Contributors to Heavy Rainfall Events in the Arkansas-Red River Basin

    OpenAIRE

    McCorkle, Taylor A.; Skylar S. Williams; Pfeiffer, Timothy A.; Basara, Jeffrey B.

    2016-01-01

    This study analyzed the top 1% 24-hour rainfall events from 1994 to 2013 at eight climatological sites that represent the east to west precipitation gradient across the Arkansas-Red River Basin in North America. A total of 131 cases were identified and subsequently classified on the synoptic-scale, mesoscale, and local-scale to compile a climatological analysis of these extreme, heavy rainfall events based on atmospheric forcings. For each location, the prominent midtropospheric pattern, meso...

  20. Molecular Subtype Analyses of Campylobacter spp. from Arkansas and California Poultry Operations

    OpenAIRE

    Hiett, K. L; Stern, N. J.; Fedorka-Cray, P.; Cox, N. A.; Musgrove, M. T.; Ladely, S.

    2002-01-01

    Campylobacter isolates from diverse samples within broiler production and processing environments were typed by using flaA short variable region DNA sequence analysis. Sixteen flocks from four different farms representing two broiler producers in Arkansas and California were analyzed. Fourteen of the flocks (87.5%) were Campylobacter-positive; two remained negative throughout the 6-week rearing period. In general, multiple clones were present within a flock. Additionally, clones found within ...

  1. Factor that Affect Arkansas Farm Operators’ and Landowners’ Decision to Participate in Agritourism

    OpenAIRE

    Rainey, Daniel V.; Djunaidi, Harjanto; McCullough, Stacey W.; Das, Biswa R.

    2010-01-01

    This study utilizes survey data analyzed at the regional level to obtain additional insights which may be left unnoticed if the analysis only considers data at the state level. The current analysis reveals that farm operators’ and/or landowners’ decision to participate in the agritourism industry in Arkansas is affected by state government infrastructure. But these infrastructures are varied among regions. The infrastructures are state government support in training the farm operators; in pro...

  2. Arthropods of Steel Creek, Buffalo National River, Arkansas. III. Heteroptera (Insecta: Hemiptera)

    OpenAIRE

    Skvarla, Michael Joseph; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the third in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and B...

  3. Terrestrial arthropods of Steel Creek, Buffalo National River, Arkansas. II. Sawflies (Insecta: Hymenoptera: " Symphyta ")

    OpenAIRE

    Skvarla, Michael Joseph; Smith, David R.; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the second in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and ...

  4. Terrestrial arthropods of Steel Creek, Buffalo National River, Arkansas. II. Sawflies (Insecta: Hymenoptera : " Symphyta ")

    OpenAIRE

    Skvarla, Michael Joseph; Smith, David R.; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the second in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and ...

  5. 28 CFR 71.42 - Judicial review.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Judicial review. 71.42 Section 71.42....42 Judicial review. Section 3805 of title 31, United States Code, authorizes judicial review by an... assessments under this part and specifies the procedures for such review....

  6. 34 CFR 200.71 - LEA eligibility.

    Science.gov (United States)

    2010-07-01

    ... least five percent of the LEA's total population ages 5 to 17 years, inclusive. (d) Education finance... 34 Education 1 2010-07-01 2010-07-01 false LEA eligibility. 200.71 Section 200.71 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY...

  7. 44 CFR 71.4 - Documentation.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Documentation. 71.4 Section... LEGISLATION § 71.4 Documentation. (a) In order to obtain a new policy of flood insurance for a structure which... protected area,” the owner of the structure must submit the documentation described in this section in...

  8. 28 CFR 540.71 - Procedures.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Procedures. 540.71 Section 540.71 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT CONTACT WITH... inmate may receive hardcover publications and newspaper only from the publisher, from a book club,...

  9. 40 CFR 405.71 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 405.71 Section 405.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen...

  10. 40 CFR 436.71 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 436.71 Section 436.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS MINERAL MINING AND PROCESSING POINT SOURCE CATEGORY Asbestos and...

  11. 10 CFR 71.113 - Document control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Document control. 71.113 Section 71.113 Energy NUCLEAR....113 Document control. The licensee, certificate holder, and applicant for a CoC shall establish measures to control the issuance of documents such as instructions, procedures, and drawings,...

  12. 41 CFR 105-71.132 - Equipment.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Equipment. 105-71.132...-Award Requirements/Changes, Property, and Subawards § 105-71.132 Equipment. (a) Title. Subject to the obligations and conditions set forth in this section, title to equipment acquired under a grant or...

  13. 49 CFR 397.71 - Federal standards.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Federal standards. 397.71 Section 397.71... standards. (a) A State or Indian tribe shall comply with the Federal standards under paragraph (b) of this... or may not be transported. (b) The Federal standards are as follows: (1) Enhancement of public...

  14. 27 CFR 71.2 - Liberal construction.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Liberal construction. 71.2... and Construction of Regulations § 71.2 Liberal construction. The regulations in this part shall be... Rules of Civil Procedure for the U.S. District Courts (28 U.S.C. appendix), where applicable, shall be...

  15. 21 CFR 640.71 - Manufacturing responsibility.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Manufacturing responsibility. 640.71 Section 640...) BIOLOGICS ADDITIONAL STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Source Plasma § 640.71 Manufacturing responsibility. (a) All steps in the manufacturing of Source Plasma, including donor examination,...

  16. 40 CFR 71.22 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Definitions. 71.22 Section 71.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL... drafted pursuant to section 112(i)(5)(B) of the Act and signed by a responsible company official...

  17. 40 CFR 71.24 - Permit applications.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Permit applications. 71.24 Section 71.24 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED... company name, telephone number, and address (or plant name, telephone number, and address if...

  18. 40 CFR 71.25 - Permit content.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Permit content. 71.25 Section 71.25 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) FEDERAL... company or entity that performed the analyses; (D) The analytical techniques or methods used; (E)...

  19. 46 CFR 71.20-10 - Plans.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Plans. 71.20-10 Section 71.20-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS INSPECTION AND CERTIFICATION Initial Inspection... approved by the Commandant. The procedure for submitting plans and the list of plans to be supplied is...

  20. 31 CFR 103.71 - General.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false General. 103.71 Section 103.71 Money and Finance: Treasury Regulations Relating to Money and Finance FINANCIAL RECORDKEEPING AND REPORTING... summon a financial institution or an officer or employee of a financial institution (including a...

  1. 10 CFR 71.133 - Corrective action.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Corrective action. 71.133 Section 71.133 Energy NUCLEAR....133 Corrective action. The licensee, certificate holder, and applicant for a CoC shall establish... determined and corrective action taken to preclude repetition. The identification of the...

  2. Outbreak of Enterovirus - 71 Meningitis in Calicut

    Directory of Open Access Journals (Sweden)

    CK Sasidharan

    2016-04-01

    Full Text Available Objective: Enterovirus 71(EV 71 causes wide spectrum of infections ranging from asymptomatic conditions to clinical syndromes like diarrhea, rash, hand-foot-and mouth disease (HFMD, herpangina, aseptic meningitis, encephalitis, myocarditis, acute flaccid paralysis, bulbar and brainstem encephalitis Guillain Barre syndrome, pulmonary haemorrhage. This study deals with an outbreak of aseptic meningitis in children caused by EV 71 virus. Methods: The authors report an outbreak of aseptic meningitis in children in and around Calicut in June 2008. Clinical and laboratory study was done in collaboration with National Centre for Disease Control, New Delhi. 149 children with aseptic meningitis were studied and followed up from June 2008 to May 2009. Result: All children had clinical features suggestive of aseptic meningitis and serology showed the rising antibody titre against EV 71 virus infection. CSF analysis also showed four fold rise in antibodies in one and ≥ 1:2 neutralising antibodies titer against EV- 71 in four samples indicating meningitis due to EV-71. Conclusion: EV 71 was identified as the causative agent of the outbreak of aseptic meningitis in the study and the fact that the EV 71 infection has evolved from minor illness like HFMD to major illness like aseptic meningitis from the same locality is truly alarming.

  3. Challenges to licensure of enterovirus 71 vaccines.

    Directory of Open Access Journals (Sweden)

    Min-Shi Lee

    Full Text Available Human enteroviruses usually cause self-limited infections except polioviruses and enterovirus 71 (EV71, which frequently involve neurological complications. EV71 vaccines are being evaluated in humans. However, several challenges to licensure of EV71 vaccines need to be addressed. Firstly, EV71 and coxsackievirus A (CA are frequently found to co-circulate and cause hand-foot-mouth disease (HFMD. A polyvalent vaccine that can provide protection against EV71 and prevalent CA are desirable. Secondly, infants are the target population of HFMD vaccines and it would need multi-national efficacy trials to prove clinical protection and speed up the licensure and usage of HFMD vaccines in children. An international network for enterovirus surveillance and clinical trials is urgently needed. Thirdly, EV71 is found to evolve quickly in the past 15 years. Prospective cohort studies are warranted to clarify clinical and epidemiological significances of the antigenic and genetic variations between different EV71 genogroups, which is critical for vaccine design.

  4. 9 CFR 145.71 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Definitions. 145.71 Section 145.71 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for...

  5. Notes from the Field: Cluster of Tuberculosis Cases Among Marshallese Persons Residing in Arkansas - 2014-2015.

    Science.gov (United States)

    Rothfeldt, Laura Lester; Patil, Naveen; Haselow, Dirk T; Williams, Sandy Hainline; Wheeler, J Gary; Mukasa, Leonard N

    2016-01-01

    During early September 2014, the Arkansas Department of Health identified an increased number of tuberculosis (TB) cases among a unique population in a well-circumscribed geographical area in northwest Arkansas. The Compact of Free Association Act of 1985 (Public Law 99-239, amended in 2003 by Public Law 108-188) established the Republic of the Marshall Islands (RMI) as an independent nation, and persons from the RMI can travel freely (with valid RMI passport) to and from the United States as nonimmigrants without visas (1). Marshallese started settling in northwest Arkansas during the early 1990s because of employment and educational opportunities (2). According to the 2010 Census, an estimated 4,300 Marshallese resided in Arkansas (2), mostly within one county which ranked 6th in the United States for counties with the highest percentage of Native Hawaiians and Other Pacific Islanders (3). It is estimated that this number has been growing steadily each year since the 2010 Census; however, obtaining an accurate count is difficult. The RMI is a TB high-incidence country, with a case-rate of 212.7 per 100,000 persons for 2014, whereas the case-rate was 3.1 per 100,000 persons in Arkansas and 2.9 per 100,000 persons in the United States (4,5). Screening for either active TB or latent TB infection (LTBI) is not required for Marshallese entry to the United States (1). PMID:27560201

  6. The Natural Divisions of Arkansas: A Three Week Unit. Classroom Guide. Recommended for High School Level Social Studies, Earth Science and Biological Science Classes.

    Science.gov (United States)

    Foti, Thomas L.

    This unit is designed to (1) acquaint the student with Arkansas, (2) provide information on what an environment (natural system) is, and (3) provide information on how people relate to the environment as a whole. Natural systems and natural divisions (geographical areas) of Arkansas are described. Included in several sections are questions to…

  7. 40 CFR 279.71 - Prohibitions.

    Science.gov (United States)

    2010-07-01

    ... THE MANAGEMENT OF USED OIL Standards for Used Oil Fuel Marketers § 279.71 Prohibitions. A used oil fuel marketer may initiate a shipment of off-specification used oil only to a used oil burner who:...

  8. Crystal Structure of Human Enterovirus 71

    OpenAIRE

    Plevka, Pavel; Perera, Rushika; Cardosa, Jane; Kuhn, Richard J.; Rossmann, Michael G.

    2012-01-01

    Enterovirus 71 is a picornavirus associated with fatal neurological illness in infants and young children. Here we report the crystal structure of enterovirus 71 and show that, unlike in other enteroviruses, the “pocket factor”, a small molecule that stabilizes the virus, is partly exposed on the floor of the canyon. Thus the structure of antiviral compounds may require a hydrophilic head group designed to interact with residues at the entrance of the pocket.

  9. Crystal Structure of Human Enterovirus 71

    Energy Technology Data Exchange (ETDEWEB)

    Plevka, Pavel; Perera, Rushika; Cardosa, Jane; Kuhn, Richard J.; Rossmann, Michael G. (Purdue); (Sentinext)

    2013-04-08

    Enterovirus 71 is a picornavirus associated with fatal neurological illness in infants and young children. Here, we report the crystal structure of enterovirus 71 and show that, unlike in other enteroviruses, the 'pocket factor,' a small molecule that stabilizes the virus, is partly exposed on the floor of the 'canyon.' Thus, the structure of antiviral compounds may require a hydrophilic head group designed to interact with residues at the entrance of the pocket.

  10. Development of Novel Vaccines against Enterovirus-71

    Directory of Open Access Journals (Sweden)

    Pinn Tsin Isabel Yee

    2015-12-01

    Full Text Available The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71 and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and treatment agents against EV-A71 to prevent further fatalities. Research groups have developed experimental inactivated vaccines, recombinant Viral Protein 1 (VP1 vaccine and virus-like particles (VLPs. The inactivated EV-A71 vaccine is considered the safest viral vaccine, as there will be no reversion to the infectious wild type strain. The recombinant VP1 vaccine is a cost-effective immunogen, while VLPs contain an arrangement of epitopes that can elicit neutralizing antibodies against the virus. As each type of vaccine has its advantages and disadvantages, increased studies are required in the development of such vaccines, whereby high efficacy, long-lasting immunity, minimal risk to those vaccinated, safe and easy production, low cost, dispensing the need for refrigeration and convenient delivery are the major goals in their design.

  11. Development of Novel Vaccines against Enterovirus-71.

    Science.gov (United States)

    Yee, Pinn Tsin Isabel; Poh, Chit Laa

    2016-01-01

    The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71) and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and treatment agents against EV-A71 to prevent further fatalities. Research groups have developed experimental inactivated vaccines, recombinant Viral Protein 1 (VP1) vaccine and virus-like particles (VLPs). The inactivated EV-A71 vaccine is considered the safest viral vaccine, as there will be no reversion to the infectious wild type strain. The recombinant VP1 vaccine is a cost-effective immunogen, while VLPs contain an arrangement of epitopes that can elicit neutralizing antibodies against the virus. As each type of vaccine has its advantages and disadvantages, increased studies are required in the development of such vaccines, whereby high efficacy, long-lasting immunity, minimal risk to those vaccinated, safe and easy production, low cost, dispensing the need for refrigeration and convenient delivery are the major goals in their design. PMID:26729152

  12. Land use change detection with LANDSAT-2 data for monitoring and predicting regional water quality degradation. [Arkansas

    Science.gov (United States)

    Macdonald, H.; Steele, K. (Principal Investigator); Waite, W.; Rice, R.; Shinn, M.; Dillard, T.; Petersen, C.

    1977-01-01

    The author has identified the following significant results. Comparison between LANDSAT 1 and 2 imagery of Arkansas provided evidence of significant land use changes during the 1972-75 time period. Analysis of Arkansas historical water quality information has shown conclusively that whereas point source pollution generally can be detected by use of water quality data collected by state and federal agencies, sampling methodologies for nonpoint source contamination attributable to surface runoff are totally inadequate. The expensive undertaking of monitoring all nonpoint sources for numerous watersheds can be lessened by implementing LANDSAT change detection analyses.

  13. Aerial gamma ray and magnetic survey: Mississippi and Florida airborne survey, Russellville quadrangle, Arkansas

    International Nuclear Information System (INIS)

    The Russellville quadrangle in north central Arkansas overlies thick Paleozoic sediments of the Arkoma Basin. These Paleozoics dominate surface exposure except where covered by Quaternary alluvial materials. Examination of available literature shows no known uranium deposits (or occurrences) within the quadrangle. Eighty-eight groups of uranium samples were defined as anomalies and are discussed briefly. None were considered significant, and most appeared to be of cultural origin. Magnetic data show character that suggest structural and/or lithologic complexity, but imply relatively deep-seated sources

  14. NSSS design and cycle 1 operating history data for Arkansas Nuclear One, Unit-2. Final report

    International Nuclear Information System (INIS)

    This report contains design and cycle 1 operating data for the Arkansas Nuclear One, Unit-2 nuclear steam supply system. The design data include descriptions of the reactor core, reactor coolant system, and control systems which are a part of the nuclear steam supply system. Operating history data are provided for the period of December 1978 through January 1980. The most important operating history data provided include reactor power, cumulative fuel burnup, control rod position, primary coolant temperature, and a series of power distribution state points

  15. Analytical data for geologic units in Missouri and parts of Kansas, Oklahoma and Arkansas

    Science.gov (United States)

    Boerngen, Josephine G.; VanTrump, George; Ebens, Richard J.

    1975-01-01

    This report lists the geochemical data collected during the course of a reconnaissance study of compositional variation in the bedrock and surficial gelogic untis exposed in Missouri and adjoining parts of Kansas, Oklahoma, and Arkansas.  The primar object of the study was to assess scale-related components of geochemical variance with a view of determining the importance of regional geochemical variation in these units (cf. Connor and other, 1972).  Geologic evaluation, geochemical interpretation and summaries, and sampling designs employed in the collection of most of these data may be found in U.S. Geological Survey (1972a-1972f, 1973) and Connor and Shacklette (1975).

  16. EV71 vaccine, an invaluable gift for children

    Science.gov (United States)

    Liang, Zhenglun; Wang, Junzhi

    2014-01-01

    Enterovirus 71 (EV71) is a major pathogen for severe hand, foot and mouth disease (HFMD). Development of vaccines against EV71 would be the most effective approach to prevent the EV71 outbreak. Research and development (R&D) of EV71 vaccine was carried out in several Asian countries. Currently three companies in mainland China have completed Phase III clinical trials of inactivated EV71 whole-virus vaccines, whereas the other two companies have completed Phase I clinical trials separately in Taiwan and in Singapore. Results from those clinical trials have indicated high safety and immunogenicity of EV71 vaccine. Protective efficacies were over 90% on EV71-associated HFMD and over 80% on other EV71-associated diseases. In this paper, we summarize the results from three EV71 vaccine Phase III clinical trials and discuss the challenges of incorporating EV71 vaccine into Expanded Program on Immunization (EPI) in countries with EV71 epidemics. PMID:25505956

  17. COPI is required for enterovirus 71 replication.

    Directory of Open Access Journals (Sweden)

    Jianmin Wang

    Full Text Available Enterovirus 71 (EV71, a member of the Picornaviridae family, is found in Asian countries where it causes a wide range of human diseases. No effective therapy is available for the treatment of these infections. Picornaviruses undergo RNA replication in association with membranes of infected cells. COPI and COPII have been shown to be involved in the formation of picornavirus-induced vesicles. Replication of several picornaviruses, including poliovirus and Echovirus 11 (EV11, is dependent on COPI or COPII. Here, we report that COPI, but not COPII, is required for EV71 replication. Replication of EV71 was inhibited by brefeldin A and golgicide A, inhibitors of COPI activity. Furthermore, we found EV71 2C protein interacted with COPI subunits by co-immunoprecipitation and GST pull-down assay, indicating that COPI coatomer might be directed to the viral replication complex through viral 2C protein. Additionally, because the pathway is conserved among different species of enteroviruses, it may represent a novel target for antiviral therapies.

  18. MRI of Enterovirus 71 myelitis with monoplegia

    International Nuclear Information System (INIS)

    We report two boys diagnosed as having herpangina and hand-foot-mouth disease complicated by monoplegia during the outbreak enterovirus infection in Taiwan in 1998. Enterovirus 71 was identified in the stool and throat swab; neither polio nor Coxsackie viruses was identified. MRI showed unilateral lesions in the anterior horns of the spinal cord at T11-12 and C2-5. Although the MRI findings and sites of these lesions were similar to those of poliovirus-associated poliomyelitis, the virological data indicated that these boys were infected with enterovirus type 71. (orig.)

  19. MRI of Enterovirus 71 myelitis with monoplegia

    Energy Technology Data Exchange (ETDEWEB)

    Shen, W.C. [Department of Radiology, and School of Medicine, China Medical College, China Medical College Hospital, Taiwan (Taiwan); Tsai, C.H. [Department of Paediatrics, and School of Medicine, China Medical College, China Medical College Hospital, Taiwan (Taiwan); Chiu, H.H. [Department of Paediatrics, China Medical College Hospital, No. 2 Yuh-Der Road, 407 Taichung, Taiwan (Taiwan); Chow, K.C. [Department of Medical Research, China Medical College Hospital, Taichung, Taiwan (Taiwan)

    2000-02-01

    We report two boys diagnosed as having herpangina and hand-foot-mouth disease complicated by monoplegia during the outbreak enterovirus infection in Taiwan in 1998. Enterovirus 71 was identified in the stool and throat swab; neither polio nor Coxsackie viruses was identified. MRI showed unilateral lesions in the anterior horns of the spinal cord at T11-12 and C2-5. Although the MRI findings and sites of these lesions were similar to those of poliovirus-associated poliomyelitis, the virological data indicated that these boys were infected with enterovirus type 71. (orig.)

  20. A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema.

    Science.gov (United States)

    Victorio, Carla Bianca Luena; Xu, Yishi; Ng, Qimei; Chua, Beng Hooi; Alonso, Sylvie; Chow, Vincent T K; Chua, Kaw Bing

    2016-01-01

    Enterovirus 71 (EV-A71) is a neurotropic virus that sporadically causes fatal neurologic illness among infected children. Animal models of EV-A71 infection exist, but they do not recapitulate in animals the spectrum of disease and pathology observed in fatal human cases. Specifically, neurogenic pulmonary oedema (NPE)-the main cause of EV-A71 infection-related mortality-is not observed in any of these models. This limits their utility in understanding viral pathogenesis of neurologic infections. We report the development of a mouse model of EV-A71 infection displaying NPE in severely affected animals. We inoculated one-week-old BALB/c mice with an adapted EV-A71 strain and identified clinical signs consistent with observations in human cases and other animal models. We also observed respiratory distress in some mice. At necropsy, we found their lungs to be heavier and incompletely collapsed compared to other mice. Serum levels of catecholamines and histopathology of lung and brain tissues of these mice strongly indicated onset of NPE. The localization of virally-induced brain lesions also suggested a potential pathogenic mechanism for EV-A71-induced NPE. This novel mouse model of virally-induced NPE represents a valuable resource for studying viral mechanisms of neuro-pathogenesis and pre-clinical testing of potential therapeutics and prophylactics against EV-A71-related neurologic complications. PMID:27357918

  1. Terrestrial arthropods of Steel Creek, Buffalo National River, Arkansas. II. Sawflies (Insecta: Hymenoptera: "Symphyta")

    Science.gov (United States)

    Smith, David R.; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the second in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and Berlese-Tullgren extraction of leaf litter. New information We provide collection records for 47 species of "Symphyta" (Insecta: Hymenoptera), 30 of which are new state records for Arkansas: (Argidae) Sterictiphora serotina; (Cimbicidae) Abia americana; (Diprionidae) Monoctenus fulvus; (Orussidae) Orussus terminalis; (Pamphiliidae) Onycholyda luteicornis, Pamphilius ocreatus, P. persicum, P. rileyi; (Pergidae) Acordulecera dorsalis, A. mellina, A. pellucida; (Tenthredinidae) Caliroa quercuscoccineae, Empria coryli, Hoplocampa marlatti, Macrophya cassandra, Monophadnoides conspiculatus, Monophadnus bakeri, Nematus abbotii, Neopareophora litura, Pachynematus corniger, Paracharactus rudis, Periclista marginicollis, Pristiphora banski, P. chlorea, Strongylogaster impressata, S. remota, Taxonus epicera, Thrinax albidopictus, T. multicinctus, Zaschizonyx montana; (Xiphydriidae) Xiphydria tibialis. PMID:27222635

  2. 5th Bionanotox and Applications International Research Conference, Peabody, Little Rock, Arkansas, USA

    Science.gov (United States)

    Sabb, Taneicie; Chowdhury, Parimal

    2011-06-01

    "BioNanoTox and Toxicity: using Technology to Advance Discovery" was this year's theme at the 5th BioNanoTox and Applications International Research Conference held at the Peabody Hotel, Little Rock, Arkansas on November 4-5th, 2010. This year, the international participation in this conference increased to 25 countries spanning the globe. The conference began with opening remarks by Paul Howard, Associate Director of the National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas, United States. Two keynote speakers, Dr. Ananth V. Annapragada and Dr. Merle G. Paule presented lectures on "Toxicity of Novel Nanoparticles for CT imaging" and "The Biology of Neurotoxicity: using Technology to Advance Discovery", respectively. Teachers, students, faculty, and scientists presented oral and poster presentations on fundamental and translational research related to BioNanoTox and related fields of science. Six presentation sessions were held over the two-day conference. There were 31 presentations and 39 posters from disciplines ranging from biology to chemistry, toxicology, nanotechnology, computational sciences, mathematics, engineering, plant science, and biotechnology. Poster presentation awards were presented to three high school students, three high school teachers, and three college students. In addition to poster awards a memorial, travel, and BioNanoTox award were presented. This year's meeting paved the way for a more outstanding meeting for the future.

  3. Water quality of least-impaired lakes in eastern and southern Arkansas.

    Science.gov (United States)

    Justus, Billy

    2010-09-01

    A three-phased study identified one least-impaired (reference) lake for each of four Arkansas lake classifications: three classifications in the Mississippi Alluvial Plain (MAP) ecoregion and a fourth classification in the South Central Plains (SCP) ecoregion. Water quality at three of the least-impaired lakes generally was comparable and also was comparable to water quality from Kansas and Missouri reference lakes and Texas least-impaired lakes. Water quality of one least-impaired lake in the MAP ecoregion was not as good as water quality in other least-impaired lakes in Arkansas or in the three other states: a probable consequence of all lakes in that classification having a designated use as a source of irrigation water. Chemical and physical conditions for all four lake classifications were at times naturally harsh as limnological characteristics changed temporally. As a consequence of allochthonous organic material, oxbow lakes isolated within watersheds comprised of swamps were susceptible to low dissolved oxygen concentrations to the extent that conditions would be limiting to some aquatic biota. Also, pH in lakes in the SCP ecoregion was black water systems. Water quality of the deepest lakes exceeded that of shallow lakes. N/P ratios and trophic state indices may be less effective for assessing water quality for shallow lakes (<2 m) than for deep lakes because there is an increased exposure of sediment (and associated phosphorus) to disturbance and light in the former. PMID:19705289

  4. Survey of pathogens and parasitoids of Popillia japonica (Coleoptera: Scarabaeidae) in northwest Arkansas.

    Science.gov (United States)

    Petty, B M; Johnson, D T; Steinkraus, D C

    2012-09-15

    The impact of pathogens and parasitoids on the recently established population of Popillia japonica Newman in northwest Arkansas has been unknown. In this study, we quantified the prevalence of natural enemies: Stictospora villani Hays, Ovavesicula popilliae Andreadis and Hanula, Paenibacillus spp. (Dingman), nematodes and parasitic Diptera and Hymenoptera in third instar and adult populations in 2010 and 2011. S. villani was found in 38.4% and 35.5% of larvae in 2010 and 2011, respectively. S. villani was not found in adult beetles. Paenibacillus bacteria were not found in either larvae or adults in either year. In 2010, the microsporidian O. popilliae was not found in larvae but was present in 0.2% of adults. In 2011, 2.6% of larvae were infected with O. popilliae, but the microsporidian was not found in adults. A previously unknown Adelina sp. was found infecting 0.4% of adult beetles in 2010 and 1.3% of larvae in 2011. Nematode infections were found in 1.8% of larvae and 0.1% of adults in 2010 and not found in either life stage in 2011. No parasitic Hymenoptera or Diptera were found in either year. Apparently, pathogens and parasitoids currently provide little control of the Japanese beetle population within northwest Arkansas. PMID:22721914

  5. Curriculum alignment and higher order thinking in introductory biology in Arkansas public two-year colleges

    Science.gov (United States)

    Crandall, Elizabeth Diane

    This dissertation identified the cognitive levels of lecture objectives, lab objectives, and test questions in introductory majors' biology. The study group included courses offered by 27 faculty members at 18 of the 22 community colleges in Arkansas. Using Bloom's Taxonomy to identify cognitive levels, the median lecture learning outcomes were at level 2 (Comprehension) and test assessments at Level 1 (Knowledge). Lab learning outcomes were determined to have a median of level 3 (Analysis). A correlation analysis was performed using SPSS software to determine if there was an association between the Bloom's level of lecture objectives and test assessments. The only significant difference found was at the Analysis level, or Bloom's level 4 (p=.043). Correlation analyses were run for two other data sets. Years of college teaching experience and hours of training in writing objectives and assessments were compared to the Bloom's Taxonomy level of lecture objectives and test items. No significant difference was found for either of these independent variables. This dissertation provides Arkansas two-year college biology faculty with baseline information about the levels of cognitive skills that are required in freshman biology for majors courses. It can serve to initiate conversations about where we are compared to a national study, where we need to be, and how we get there.

  6. User's manual for FORAR: a stand model for composition and growth of upland forests of southern Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Mielke, D. L.; Shugart, H. H.; West, D. C.

    1977-04-01

    This report is a user's manual for FORAR, a computer model simulating stand growth and composition of upland forests of south central Arkansas. The model computes: the number and biomass of each tree species, and the dbh, age, and species of each individual tree on a 1/12-ha circular plot.

  7. 40 CFR 60.71 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... subpart A of this part. (a) Nitric acid production unit means any facility producing weak nitric acid by either the pressure or atmospheric pressure process. (b) Weak nitric acid means acid which is 30 to 70... PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Nitric Acid Plants § 60.71 Definitions....

  8. 41 CFR 105-71.102 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... (Continued) GENERAL SERVICES ADMINISTRATION Regional Offices-General Services Administration 71-UNIFORM... equipment means the net invoice unit price of the property including the cost of modifications, attachments... under the United States Housing Act of 1937) school district, special district, intrastate...

  9. 21 CFR 71.1 - Petitions.

    Science.gov (United States)

    2010-04-01

    ... use of the color additive in packaging material, the petitioner shall show how this may occur and what... AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVE PETITIONS General Provisions § 71.1 Petitions. (a) Any interested person may propose the listing of a color...

  10. 48 CFR 246.408-71 - Aircraft.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Aircraft. 246.408-71... Aircraft. (a) The Federal Aviation Administration (FAA) has certain responsibilities and prerogatives in connection with some commercial aircraft and of aircraft equipment and accessories (Pub. L. 85-726 (72...

  11. SR-71 - In-flight from Tanker

    Science.gov (United States)

    1994-01-01

    Dryden's SR-71B, NASA 831, slices across the snow-covered southern Sierra Nevada Mountains of California after being refueled by an Air Force tanker during a 1994 flight. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or LASRE Experiment. Another earlier project consisted of a series of flights using the SR-71 as a science camera platform for NASA's Jet Propulsion Laboratory in Pasadena, California. An upward

  12. 9 CFR 71.1 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... Note: For Federal Register citations affecting § 71.1, see the List of CFR Sections Affected, which... description of any individual or group identification associated with the swine, the name of the swine... system. Tick infested. Infested with the ticks Boophilus annulatus (Margaropus annulatus),...

  13. SR-71 - Taxi on Ramp with Engines

    Science.gov (United States)

    1995-01-01

    This photo shows a head-on shot of NASA's SR-71A aircraft taxiing on the ramp at NASA's Dryden Flight Research Center, Edwards, California, heat waves from its engines blurring the hangars in the background. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or LASRE Experiment. Another earlier project consisted of a series of flights using the SR-71 as a science camera platform for NASA's Jet Propulsion Laboratory in Pasadena

  14. 48 CFR 1503.500-71 - Procedures.

    Science.gov (United States)

    2010-10-01

    ... IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Contractor Responsibility To Avoid Improper Business Practices 1503.500-71 Procedures. (a) A contractor's system of management controls should provide for: (1) A written code of business ethics and conduct and an ethics training program for...

  15. Description of the B-71 library system

    International Nuclear Information System (INIS)

    The B-71 system of libraries for programming in machine codes for M-220 and BESM-4 computers is described. 22 libraries are described containing more than 50 programs both newly created libraries and libraries included in the B-61 system

  16. 27 CFR 71.4 - Delegations of the Administrator.

    Science.gov (United States)

    2010-04-01

    ... officers are specified in TTB Order 1135.71, Delegation of the Administrator's Authorities in 27 CFR Part... Administrator. 71.4 Section 71.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... and Construction of Regulations § 71.4 Delegations of the Administrator. Most of the...

  17. 48 CFR 552.246-71 - Source Inspection by Government.

    Science.gov (United States)

    2010-10-01

    ... Government. 552.246-71 Section 552.246-71 Federal Acquisition Regulations System GENERAL SERVICES....246-71 Source Inspection by Government. As prescribed in 546.302-71, insert the following clause: Source Inspection by Government (JUNE 1, 2009) (a) Inspection by Government personnel. (1) Supplies to...

  18. A Non-Mouse-Adapted Enterovirus 71 (EV71) Strain Exhibits Neurotropism, Causing Neurological Manifestations in a Novel Mouse Model of EV71 Infection

    OpenAIRE

    Khong, Wei Xin; Yan, Benedict; Yeo, Huimin; Tan, Eng Lee; Lee, Jia Jun; Ng, Jowin K. W.; Chow, Vincent T.; Alonso, Sylvie

    2012-01-01

    Enterovirus 71 (EV71) is a neurotropic pathogen that has been consistently associated with the severe neurological forms of hand, foot, and mouth disease. The lack of a relevant animal model has hampered our understanding of EV71 pathogenesis, in particular the route and mode of viral dissemination. It has also hindered the development of effective prophylactic and therapeutic approaches, making EV71 one of the most pressing public health concerns in Southeast Asia. Here we report a novel mou...

  19. Construction and characterization of infectious cDNA clones of enterovirus 71(EV71)

    Institute of Scientific and Technical Information of China (English)

    Ying-wei; Ma; Shu-bin; Hao; Le-le; Sun; Jing; Li; Qiao; Qiao; Feng; Gao; Li; Zhao; Xue-jie; Yu; Zhi-yu; Wang; Hong-ling; Wen

    2015-01-01

    Dear Editor,Enterovirus 71(EV71),a member of the genus Enterovirus of the family Picornaviridae,is a single-stranded,positive-sense RNA virus that usually causes mild handfoot-mouth disease(HFMD)in children,with symptoms such as fever,diarrhea,and herpangina(Liu et al.,2013).However,certain strains of EV71 infection can cause severe neurological complications,such as SDLY107(Sun et al.,2014).EV71 is classified into three distinct genotypes(A–C);the B and C genotypes are further divided into B1–B5 and C1–C5 genotypes,respectively,based on

  20. Uranium Resource Evaluation Project. Hydrochemical and stream-sediment reconnaissance basic data for Ardmore, McAlester, Little Rock, and Helena Quadrangles, Oklahoma; Arkansas; Mississippi

    International Nuclear Information System (INIS)

    Data are compiled for hydrochemical and stream sediment reconnaisance of the following quadrangles: Ardmore, McAlester, Little Rock and Helena. These quadrangles are in Oklahoma, Arkansas and Mississippi

  1. Geodatabase of the available top and bottom surface datasets that represent the Edwards-Trinity aquifer system, Arkansas, Oklahoma, and Texas

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This geodatabase contains the spatial datasets that represent the Edwards-Trinity aquifer system in the States of Arkansas, Oklahoma, and Texas. Included are: (1)...

  2. A 71 Sector CGE Model for Germany

    OpenAIRE

    Andreas Mense; Andreas Mense, DIW Berlin/FAU Erlangen-Nürnberg; Konstanin Kholodilin, Ph.D, DIW Berlin

    2011-01-01

    A sectorally disaggregated CGE model is a powerful tool for economic policy simulation. Using the sectoral data, that are encompassing 71 sectors/commodities taken from the most recent input-output table refering to 2007, we were able to construct a detailed general equilibrium model for Germany at the national level. This model allows us to simulate the impact of different shocks on the output and employment in virtually all sectors of German economy. The possibilities of a further developme...

  3. Demographics of Same-sex Couples in Arkansas, Missouri, Nebraska, North Dakota, and South Dakota: Analyses of the 2013 American Community Survey

    OpenAIRE

    Gates, Gary J.

    2015-01-01

    Analyzing data from the 2013 US American Community Survey, this report considers the demographic, economic, and geographic characteristics of same-sex couples (married and unmarried), especially those raising children, in Arkansas, Missouri, Nebraska, North Dakota, and South Dakota. Comparisons are made with their different-sex counterparts. In Arkansas, Missouri, Nebraska, North Dakota, and South Dakota, as of 2013, there are an estimated 19652 same-sex couples. An estimated 12% of these cou...

  4. A COLLABORATIVE LEARNING MATRIX FOR COMBINING SCIENCE WITH STAKEHOLDER INVOLVEMENT TO PRIORITIZE WATERSHED IMPLEMENTATION IN ARKANSAS' NONPOINT SOURCE STATE MANAGEMENT PLAN

    OpenAIRE

    ROBERT MORGAN; MARTY MATLOCK

    2008-01-01

    In 2004, the Ecological Engineering Group at the University of Arkansas received a grant to update Arkansas' nonpoint source pollution (NPS) management program. A stakeholder involvement process was developed that used collaborative learning (CL) and comparative risk assessment (CRA) to prioritise watersheds for NPS implementation. The relative ecological risk posed by nonpoint pollution to each watershed was assessed and values assigned using available water quality, GIS, and demographic dat...

  5. Size distribution of planktonic autotrophy and microheterotrophy in DeGray Reservoir, Arkansas

    International Nuclear Information System (INIS)

    Naturally occurring assemblages of phytoplankton and bacterioplankton were radiolabelled with sodium 14C-bicarbonate and sodium 3H-acetate and size fractionated to determine the size structure of planktonic autotrophy and microheterotrophy in DeGray Reservoir, an oligotrophic impoundment of the Caddo River in south-central Arkansas. Size distributions of autotrophy and microheterotrophy were remarkably uniform seasonally, vertically within the water column, and along the longitudinal axis of the reservoir despite significant changes in environmental conditions. Planktonic autotrophy was dominated by small algal cells with usually >50% of the photosynthetic carbon uptake accounted for by organisms 75% of the planktonic microheterotrophy. Longitudinal patterns in autotrophic and microheterotrophic activities associated with >3-μm and >1-μm size fractions, respectively, suggest an uplake to downlake shift from riverine to lacustrine environmental influences within the reservoir. 83 references, 7 figures

  6. Digital surfaces and thicknesses of selected hydrogeologic units within the Ozark Plateaus aquifer system, northwestern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Bolyard, Susan E.; Hart, Rheannon M.; Clark, Jimmy M.

    2014-01-01

    Digital surfaces and thicknesses of nine hydrogeologic units of the Ozark Plateaus aquifer system from land surface to the top of the Gunter Sandstone in northwestern Arkansas were created using geophysical logs, drillers’ logs, geologist-interpreted formation tops, and previously published maps. The 6,040 square mile study area in the Ozark Plateaus Province includes Benton, Washington, Carroll, Madison, Boone, Newton, Marion, and Searcy Counties. The top of each hydrogeologic unit delineated on geophysical logs was based partly on previously published reports and maps and also from drillers’ logs. These logs were then used as a basis to contour digital surfaces showing the top and thickness of the Fayetteville Shale, the Boone Formation, the Chattanooga Shale, the Everton Formation, the Powell Dolomite, the Cotter Dolomite, the Roubidoux Formation, the Gasconade Dolomite, and the Gunter Sandstone.

  7. Quality aspects in nuclear engineering courses at the University of Arkansas

    International Nuclear Information System (INIS)

    Although quality assurance and total quality management are well-established programs in industry, almost all university academic programs lack formally organized programs for development, demonstration, and maintenance of high quality. Many academic programs do have many facets of a quality assurance program, it is simply handled as a part of the usual management of the academic program. These quality assurance programs inevitably are aimed at management of the instructor, with little or no emphasis on the ongoing quality of student work. This paper describes how the concept of quality is directed toward the entire aspect of nuclear engineering classes at the University of Arkansas, from overall university management of the instructor to details concerning instructor contact with students to improve the quality of the student's own work. One particular new concept is introduced: the use of quality points by the author in grading all students work

  8. Geologic map of the west-central Buffalo National River region, northern Arkansas

    Science.gov (United States)

    Hudson, Mark R.; Turner, Kenzie J.

    2014-01-01

    This map summarizes the geology of the west-central Buffalo National River region in the Ozark Plateaus region of northern Arkansas. Geologically, the region lies on the southern flank of the Ozark dome, an uplift that exposes oldest rocks at its center in Missouri. Physiographically, the map area spans the Springfield Plateau, a topographic surface generally held up by Mississippian cherty limestone and the higher Boston Mountains to the south, held up by Pennsylvanian rocks. The Buffalo River flows eastward through the map area, enhancing bedrock erosion of an approximately 1,600-ft- (490-m-) thick sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. Quaternary surficial units are present as alluvial deposits along major streams, including a series of terrace deposits from the Buffalo River, as well as colluvium and landslide deposits mantling bedrock on hillslopes.

  9. Middle school transition and body weight outcomes: Evidence from Arkansas Public Schoolchildren.

    Science.gov (United States)

    Zeng, Di; Thomsen, Michael R; Nayga, Rodolfo M; Rouse, Heather L

    2016-05-01

    There is evidence that middle school transition adversely affects educational and psychological outcomes of pre-teen children, but little is known about the impacts of middle school transition on other aspects of health. In this article, we estimate the impact of middle school transition on the body mass index (BMI) of public schoolchildren in Arkansas, United States. Using an instrumental variable approach, we find that middle school transition in grade 6 led to a moderate decrease of 0.04 standard deviations in BMI z-scores for all students. Analysis by subsample indicated that this result was driven by boys (0.06-0.07 standard deviations) and especially by non-minority boys (0.09 standard deviations). We speculate that the changing levels of physical activities associated with middle school transition provide the most reasonable explanation for this result. PMID:26794273

  10. Reciprocal Regulation between Enterovirus 71 and the NLRP3 Inflammasome

    Directory of Open Access Journals (Sweden)

    Hongbin Wang

    2015-07-01

    Full Text Available Enterovirus 71 (EV71 is the major etiological agent of hand, foot, and mouth disease (HFMD. Early studies showed that EV71-infected patients with severe complications exhibited elevated plasma levels of IL-1β, indicating that EV71 may activate inflammasomes. Our current study demonstrates that the NLRP3 inflammasome plays a protective role against EV71 infection of mice in vivo. EV71 replication in myeloid cells results in the activation of the NLRP3 inflammasome and secretion of IL-1β. Conversely, EV71 counteracts inflammasome activation through cleavage of NLRP3 by viral proteases 2A and 3C, which cleave NLRP3 protein at the G493-L494 or Q225-G226 junction, respectively. Moreover, EV71 3C interacts with NLRP3 and inhibits IL-1β secretion when expressed in mammalian cells. These results thus reveal a set of reciprocal regulations between enterovirus 71 and the NLRP3 inflammasome.

  11. The road to tuberculosis (Mycobacterium tuberculosis elimination in Arkansas; a re-examination of risk groups.

    Directory of Open Access Journals (Sweden)

    Anna Berzkalns

    Full Text Available OBJECTIVES: This study was conducted to generate knowledge useful for developing public health interventions for more effective tuberculosis control in Arkansas. METHODS: The study population included 429 culture-confirmed reported cases (January 1, 2004-December 31, 2010. Mycobacterium tuberculosis genotyping data were used to identify cases likely due to recent transmission (clustered versus reactivation (non-clustered. Poisson regression models estimated average decline rate in incidence over time and assessed the significance of differences between subpopulations. A multinomial logistic model examined differences between clustered and non-clustered incidence. RESULTS: A significant average annual percent decline was found for the overall incidence of culture-confirmed (9%; 95% CI: 5.5%, 16.9%, clustered (6%; 95% CI: 0.5%, 11.6%, and non-clustered tuberculosis cases (12%; 95% CI: 7.6%, 15.9%. However, declines varied among demographic groups. Significant declines in clustered incidence were only observed in males, non-Hispanic blacks, 65 years and older, and the rural population. CONCLUSIONS: These findings suggest that the Arkansas tuberculosis control program must target both traditional and non-traditional risk groups for successful tuberculosis elimination. The present study also demonstrates that a thorough analysis of TB trends in different population subgroups of a given geographic region or state can lead to the identification of non-traditional risk factors for TB transmission. Similar studies in other low incidence populations would provide beneficial data for how to control and eventually eliminate TB in the U.S.

  12. Arkansas' experience: statewide surveillance and parental information on the child obesity epidemic.

    Science.gov (United States)

    Thompson, Joseph W; Card-Higginson, Paula

    2009-09-01

    Parents, clinicians, public health officials, and policy makers need readily available information on the extent of the childhood obesity epidemic. As in any epidemic, the strategies and tools used to combat the imminent threat are frequently based on scientific rationale and experience but applied in areas in which we lack complete understanding. The urgent need for information requires execution of decisions that are not risk-free--such is the case of BMI screening obesity. Use of BMI percentiles to classify weight status among youth and quantify the epidemic can inform and engage parents and other key stakeholders. Arkansas has completed its sixth year of BMI screenings for public school students. Through a groundbreaking legislative mandate that requires BMI assessments in public schools, the state has achieved both enhanced awareness among parents and their children and increased engagement by school, clinical, public health, and community leaders in response to the epidemic. External evaluations conducted since institution of BMI assessments have revealed none of the initially feared negative consequences of BMI measurements such as teasing, use of diet pills, or excessive concerns about weight. In the face of this epidemic, the risks of using BMI assessments in clinical or school-based settings must be recognized but can be managed. Arkansas' Act 1220 and BMI-reporting efforts have not only afforded parents detailed information about their children's health but also provided longitudinal data needed to fully understand the scope of childhood and adolescent obesity in the state and to track progress made in combating this epidemic. PMID:19720670

  13. Effects of recent climate variability on groundwater levels in eastern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Schrader, T.P.

    2013-01-01

    Water-level fluctuations in wells completed in the Mississippi River Valley alluvial aquifer in eastern Arkansas were compared to variability in annual precipitation, an indicator of climate variability. The wettest year on record in Little Rock, Arkansas, occurred in 2009 with 81.79 inches of precipitation compared to an average of 47.1 inches per year. In contrast, 2005 and 2010 were the 7th and 14th driest years on record with 34.55 and 36.52 inches per year, respectively. This variability in precipitation was reflected in water-level altitude changes between 2004 and 2008 and 2006 and 2010. Generally, drier conditions between 2004 and 2008 led to an average decline in water levels of 1.62 feet, whereas wetter conditions between 2006 and 2010 led to an average rise in water levels of 1.36 feet. Drier periods likely resulted in less recharge compared to wetter periods. Groundwater use from the alluvial aquifer peaked in 2000 and has since declined, in part, because of conservation measures and substantial reduction in aquifer saturated thickness. Groundwater-flow model results showed some areas of the alluvial aquifer simulated as dry in 2010, indicating a reduced capacity of the alluvial aquifer to produce water in those areas. Additional factors affecting groundwater use include the types of crops grown in an area and the availabitiliy of crop subsidies. Real-time continuous water-level measurements in wells allow for a more accurate assessment of the effect of variability in precipitation and water use than periodic water-level measurements.

  14. Development of antiviral agents toward enterovirus 71 infection.

    Science.gov (United States)

    Pourianfar, Hamid Reza; Grollo, Lara

    2015-02-01

    Enterovirus 71 (EV71) infection remains a public health problem at a global level, particularly in the Asia-Pacific region. The infection normally manifests as hand-foot-mouth disease; however, it is capable of developing into potentially fatal neurological complications. There is currently no approved vaccine or antiviral substance available for the prevention or treatment of EV71 infection. This paper, thus, reviews efforts to develop or discover synthetic as well as naturally occurring compounds directed against EV71 infection. The recent achievements in cellular receptors of EV71 are also highlighted, and their contribution to the development of antiviral drugs against EV71 is discussed in this article. PMID:24560700

  15. Crystal structures of enterovirus 71 (EV71) recombinant virus particles provide insights into vaccine design.

    Science.gov (United States)

    Lyu, Ke; Wang, Guang-Chuan; He, Ya-Ling; Han, Jian-Feng; Ye, Qing; Qin, Cheng-Feng; Chen, Rong

    2015-02-01

    Hand-foot-and-mouth disease (HFMD) remains a major health concern in the Asia-Pacific regions, and its major causative agents include human enterovirus 71 (EV71) and coxsackievirus A16. A desirable vaccine against HFMD would be multivalent and able to elicit protective responses against multiple HFMD causative agents. Previously, we have demonstrated that a thermostable recombinant EV71 vaccine candidate can be produced by the insertion of a foreign peptide into the BC loop of VP1 without affecting viral replication. Here we present crystal structures of two different naturally occurring empty particles, one from a clinical C4 strain EV71 and the other from its recombinant virus containing an insertion in the VP1 BC loop. Crystal structure analysis demonstrated that the inserted foreign peptide is well exposed on the particle surface without significant structural changes in the capsid. Importantly, such insertions do not seem to affect the virus uncoating process as illustrated by the conformational similarity between an uncoating intermediate of another recombinant virus and that of EV71. Especially, at least 18 residues from the N terminus of VP1 are transiently externalized. Altogether, our study provides insights into vaccine development against HFMD. PMID:25492868

  16. SR-71 Over Snow Capped Mountains

    Science.gov (United States)

    1995-01-01

    Dryden's SR-71B, NASA 831, slices across the snowy southern Sierra Nevada Mountains of California after being refueled by an Air Force Flight Test Center tanker during a recent flight. The Mach 3 aircraft are being flown by the Dryden Flight Research Center, Edwards, California as testbeds for high-speed, high-altitude aeronautical research. Capable of flying more than 2200 mph and at altitudes of over 85,000 feet, they are excellent platforms for research and experiments in aerodynamics, propulsion, structures, thermal protection materials, atmospheric studies, and sonic boom characterization.

  17. Aliens or the SR-71 Blackbird?

    Science.gov (United States)

    Ruiz, Michael J.

    2006-04-01

    Unidentified flying objects (UFOs) capture the interest of our students and the general public.1,2 Here is a UFO story I tell my students to illustrate the workings of science. Science, at its most fundamental level, is observation and an attempt to analyze what is observed. But what happens when an observation cannot be explained with the available knowledge at the time? What do we do? The UFO sighting of my story could not be explained until disclosure of a top-secret military aircraft, the SR-71, also known as the "Blackbird."

  18. Stanford survives 7.1 shock

    International Nuclear Information System (INIS)

    The Monday morning of 16 October looked like the start of a quiet week at the Stanford Linear Accelerator Center (SLAC). After a successful six-month physics run, the SLC Stanford Linear Collider was shut down to begin scheduled upgrades and the installation of two vertex detectors for the Mark II detector. Then at 5.04 p.m. the next day, the Earth's crust had had enough. A major earthquake measuring 7.1 on the Richter scale rocked the San Francisco Bay Area from an epicentre along the wicked San Andreas Fault in the Southern Santa Cruz mountains

  19. Protective effect of enterovirus‑71 (EV71) virus‑like particle vaccine against lethal EV71 infection in a neonatal mouse model.

    Science.gov (United States)

    Cao, Lei; Mao, Fengfeng; Pang, Zheng; Yi, Yao; Qiu, Feng; Tian, Ruiguang; Meng, Qingling; Jia, Zhiyuan; Bi, Shengli

    2015-08-01

    Enterovirus-71 (EV71) is a viral pathogen that causes severe cases of hand, foot and mouth disease (HFMD) among young children, with significant mortality. Effective vaccines against HFMD are urgently required. Several EV71 virus-like particle (VLP) vaccine candidates were found to be protective in the neonatal mouse EV71 challenge model. However, to what extent the VLP vaccine protects susceptible organs against EV71 infection in vivo has remained elusive. In the present study, the comprehensive immunogenicity of a potential EV71 vaccine candidate based on VLPs was evaluated in a neonatal mouse model. Despite lower levels of neutralizing antibodies to EV71 in the sera of VLP-immunized mice compared with those in mice vaccinated with inactivated EV71, the VLP-based vaccine was shown to be able to induce immunoglobulin (Ig)G and IgA memory-associated cellular immune responses to EV71. Of note, the EV71 VLP vaccine candidate was capable of inhibiting viral proliferation in cardiac muscle, skeletal muscle, lung and intestine of immunized mice and provided effective protection against the pathological damage caused by viral attack. In particular, the VLP vaccine was able to inhibit the transportation of EV71 from the central nervous system to the muscle tissue and greatly protected muscle tissue from infection, along with recovery from the viral infection. This led to nearly 100% immunoprotective efficacy, enabling neonatal mice delivered by VLP-immunized female adult mice to survive and grow with good health. The present study provided valuable additional knowledge of the specific protective efficacy of the EV71 VLP vaccine in vivo, which also indicated that it is a promising potential candidate for being developed into an EV71 vaccine. PMID:25936344

  20. Protective effect of enterovirus-71 (EV71) virus-like particle vaccine against lethal EV71 infection in a neonatal mouse model

    Science.gov (United States)

    CAO, LEI; MAO, FENGFENG; PANG, ZHENG; YI, YAO; QIU, FENG; TIAN, RUIGUANG; MENG, QINGLING; JIA, ZHIYUAN; BI, SHENGLI

    2015-01-01

    Enterovirus-71 (EV71) is a viral pathogen that causes severe cases of hand, foot and mouth disease (HFMD) among young children, with significant mortality. Effective vaccines against HFMD are urgently required. Several EV71 virus-like particle (VLP) vaccine candidates were found to be protective in the neonatal mouse EV71 challenge model. However, to what extent the VLP vaccine protects susceptible organs against EV71 infection in vivo has remained elusive. In the present study, the comprehensive immunogenicity of a potential EV71 vaccine candidate based on VLPs was evaluated in a neonatal mouse model. Despite lower levels of neutralizing antibodies to EV71 in the sera of VLP-immunized mice compared with those in mice vaccinated with inactivated EV71, the VLP-based vaccine was shown to be able to induce immunoglobulin (Ig)G and IgA memory-associated cellular immune responses to EV71. Of note, the EV71 VLP vaccine candidate was capable of inhibiting viral proliferation in cardiac muscle, skeletal muscle, lung and intestine of immunized mice and provided effective protection against the pathological damage caused by viral attack. In particular, the VLP vaccine was able to inhibit the transportation of EV71 from the central nervous system to the muscle tissue and greatly protected muscle tissue from infection, along with recovery from the viral infection. This led to nearly 100% immunoprotective efficacy, enabling neonatal mice delivered by VLP-immunized female adult mice to survive and grow with good health. The present study provided valuable additional knowledge of the specific protective efficacy of the EV71 VLP vaccine in vivo, which also indicated that it is a promising potential candidate for being developed into an EV71 vaccine. PMID:25936344

  1. Superfund at work: Hazardous waste cleanup efforts nationwide, spring 1993 (Old Midland Products Site Profile, Yell County, Arkansas). Fact sheet

    International Nuclear Information System (INIS)

    The Old Midland Products site in Yell County, Arkansas was contaminated by hazardous chemicals from operations of a wood preserving plant. The U.S. Environmental Protection Agency (EPA) worked closely with the Arkansas Department of Pollution Control and Ecology (ADPCE) to select a cleanup plan to remediate the site, allowing unrestricted use by 1998. EPA and ADPCE actions consisted of: treating over 11 million gallons of contaminated surface water and lagoon liquids; employing a highly efficient, transportable incinerator to destroy more than 85,000 tons of contaminated soil and lagoon sludges over a short period of time; installing recovery wells to treat an estimated 450,000 gallons of contaminated ground water; and maintaining effective community relations with local residents to address their concerns and involve them in the cleanup process. The site serves as an example of how EPA, state agencies, and local residents can work together to restore the environment polluted by hazardous waste

  2. BEACON/MOD2A analysis of the Arkansas-1 reactor cavity during a hypothetical hot leg break

    International Nuclear Information System (INIS)

    As part of the evaluation of the new MOD2A version of the BEACON code, the Arkansas-1 reactor cavity was modeled during a hypothetical loss-of-coolant accident. Results of the BEACON analysis were compared with results obtained previously with the COMPARE containment code. Studies were also made investigating some of the BEACON interphasic, timestep control, and wall heat transfer options to assure that these models were working properly and to observe their effects on the results. Descriptions of the Arkansas-1 reactor cavity, initial assumptions during the hypothetical LOCA, and methods of modeling with BEACON are presented. Some of the problems encountered in accurately modeling the penetrations surrounding the hot and cold leg pipes are also discussed

  3. The Antiviral Effect of Baicalin on Enterovirus 71 In Vitro

    Directory of Open Access Journals (Sweden)

    Xiang Li

    2015-08-01

    Full Text Available Baicalin is a flavonoid compound extracted from Scutellaria roots that has been reported to possess antibacterial, anti-inflammatory, and antiviral activities. However, the antiviral effect of baicalin on enterovirus 71 (EV71 is still unknown. In this study, we found that baicalin showed inhibitory activity on EV71 infection and was independent of direct virucidal or prophylactic effect and inhibitory viral absorption. The expressions of EV71/3D mRNA and polymerase were significantly blocked by baicalin treatment at early stages of EV71 infection. In addition, baicalin could decrease the expressions of FasL and caspase-3, as well as inhibit the apoptosis of EV71-infected human embryonal rhabdomyosarcoma (RD cells. Altogether, these results indicate that baicalin exhibits potent antiviral effect on EV71 infection, probably through inhibiting EV71/3D polymerase expression and Fas/FasL signaling pathways.

  4. Transgenic mouse model for the study of enterovirus 71 neuropathogenesis

    OpenAIRE

    Fujii, Ken; Nagata, Noriyo; Sato, Yuko; Ong, Kien Chai; Wong, Kum Thong; Yamayoshi, Seiya; Shimanuki, Midori; Shitara, Hiroshi; Taya, Choji; KOIKE, Satoshi

    2013-01-01

    EV71 infection with severe neurological complications has become a serious public health concern. However, suitable small animal models to study human EV71 pathogenesis are not available. We have generated a Tg mouse model by expressing the human EV71 receptor, Scavenger receptor B2, and found it to be susceptible to EV71 infection. This Tg mouse model exhibits neurological disease and pathology very similar to that observed in humans. The results confirm that the Scavenger receptor B2 recept...

  5. 7 CFR 1794.71 - Adoption of an EA.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 12 2010-01-01 2010-01-01 false Adoption of an EA. 1794.71 Section 1794.71... AGRICULTURE (CONTINUED) ENVIRONMENTAL POLICIES AND PROCEDURES Adoption of Environmental Documents § 1794.71 Adoption of an EA. RUS may adopt a Federal EA or EIS or a portion thereof as its EA. RUS shall make the...

  6. 20 CFR 71.6 - Notice of injury or death.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Notice of injury or death. 71.6 Section 71.6..., DISABILITY OR DEATH OF CIVILIAN AMERICAN CITIZENS INCURRED WHILE DETAINED BY OR IN HIDING FROM THE IMPERIAL JAPANESE GOVERNMENT GENERAL PROVISIONS § 71.6 Notice of injury or death. Notwithstanding any of...

  7. 36 CFR 71.11 - Collection of Federal recreation fees.

    Science.gov (United States)

    2010-07-01

    ... recreation fees. 71.11 Section 71.11 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR RECREATION FEES § 71.11 Collection of Federal recreation fees. The bureaus of the Department of the Interior administering outdoor recreation programs shall provide for the collection...

  8. 10 CFR 71.11 - Protection of Safeguards Information.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Protection of Safeguards Information. 71.11 Section 71.11... General Provisions § 71.11 Protection of Safeguards Information. Each licensee, certificate holder, or... public health and safety or the common defense and security, shall protect Safeguards Information...

  9. 46 CFR 188.10-71 - Scientific personnel.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Scientific personnel. 188.10-71 Section 188.10-71 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS Definition of Terms Used in This Subchapter § 188.10-71 Scientific personnel. This term...

  10. 5 CFR 7.1 - Discretion in filling vacancies.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Discretion in filling vacancies. 7.1 Section 7.1 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE RULES GENERAL PROVISIONS (RULE VII) § 7.1 Discretion in filling vacancies. In his discretion, an appointing officer may fill...

  11. 10 CFR 71.107 - Package design control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Package design control. 71.107 Section 71.107 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a...

  12. 14 CFR 71.7 - Bearings, radials, and mileages.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Bearings, radials, and mileages. 71.7 Section 71.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... REPORTING POINTS § 71.7 Bearings, radials, and mileages. All bearings and radials in this part are true...

  13. 12 CFR 908.71 - Practice before the Finance Board.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Practice before the Finance Board. 908.71 Section 908.71 Banks and Banking FEDERAL HOUSING FINANCE BOARD FEDERAL HOUSING FINANCE BOARD ORGANIZATION... Finance Board § 908.71 Practice before the Finance Board. Practice before the Finance Board for...

  14. 29 CFR 4044.71 - Valuation of annuity benefits.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Valuation of annuity benefits. 4044.71 Section 4044.71... ALLOCATION OF ASSETS IN SINGLE-EMPLOYER PLANS Valuation of Benefits and Assets Non-Trusteed Plans § 4044.71 Valuation of annuity benefits. The value of a benefit which is to be paid as an annuity is the cost...

  15. 10 CFR 71.127 - Handling, storage, and shipping control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Handling, storage, and shipping control. 71.127 Section 71... MATERIAL Quality Assurance § 71.127 Handling, storage, and shipping control. The licensee, certificate... handling, storage, shipping, cleaning, and preservation of materials and equipment to be used in...

  16. 7 CFR 984.71 - Reports of handler inventory.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Reports of handler inventory. 984.71 Section 984.71... Regulating Handling Reports, Books, and Other Records § 984.71 Reports of handler inventory. Each handler... or her inventory of inshell and shelled walnuts....

  17. 42 CFR 71.42 - Disinfection of imports.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Disinfection of imports. 71.42 Section 71.42 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES QUARANTINE, INSPECTION, LICENSING FOREIGN QUARANTINE Requirements Upon Arrival at U.S. Ports: Sanitary Inspection § 71.42 Disinfection...

  18. 19 CFR 123.71 - Description of program.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Description of program. 123.71 Section 123.71 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Land Border Carrier Initiative Program § 123.71 Description of program. The Land Border...

  19. 10 CFR 71.119 - Control of special processes.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Control of special processes. 71.119 Section 71.119 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.119 Control of special processes. The licensee, certificate holder, and applicant for a...

  20. 7 CFR 1944.71 - Term of grant.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 13 2010-01-01 2009-01-01 true Term of grant. 1944.71 Section 1944.71 Agriculture... REGULATIONS (CONTINUED) HOUSING Housing Application Packaging Grants § 1944.71 Term of grant. (a) For Single... specified in FmHA or its successor agency under Public Law 103-354's advertisement. Preapplications...

  1. 28 CFR 71.5 - Review by the reviewing official.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Review by the reviewing official. 71.5... Justice § 71.5 Review by the reviewing official. (a) If, based on the report of the investigating official under § 71.4(b), the reviewing official determines that there is adequate evidence to believe that...

  2. 46 CFR 71.75-15 - Posting of Convention certificates.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Posting of Convention certificates. 71.75-15 Section 71... AND CERTIFICATION Certificates Under the International Convention for Safety of Life at Sea, 1960 § 71.75-15 Posting of Convention certificates. (a) The certificates described in this subpart,...

  3. 7 CFR 1212.71 - Books and records.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Books and records. 1212.71 Section 1212.71..., Promotion, Consumer Education, and Industry Information Order Reports, Books, and Records § 1212.71 Books... maintain any books and records necessary to carry out the provisions of this part, and any...

  4. 29 CFR 71.13 - Employee standards of conduct.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Employee standards of conduct. 71.13 Section 71.13 Labor... ACT OF 1974 General § 71.13 Employee standards of conduct. (a) Each component shall inform its... maintains no system of records without public notice. (b) Except to the extent that the Privacy Act...

  5. 30 CFR 71.601 - Drinking water; quality.

    Science.gov (United States)

    2010-07-01

    ... drinking water provided shall conform to the Public Health Service Drinking Water Standards, 42 CFR part 72... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Drinking water; quality. 71.601 Section 71.601... Water § 71.601 Drinking water; quality. (a) Potable water provided in accordance with the provisions...

  6. 46 CFR 71.25-37 - Pollution prevention.

    Science.gov (United States)

    2010-10-01

    ... pollution prevention in 33 CFR part 155, subpart B. ... 46 Shipping 3 2010-10-01 2010-10-01 false Pollution prevention. 71.25-37 Section 71.25-37 Shipping... Annual Inspection § 71.25-37 Pollution prevention. At each inspection for certification, the...

  7. 36 CFR 7.1 - Colonial National Historical Park.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Colonial National Historical Park. 7.1 Section 7.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR SPECIAL REGULATIONS, AREAS OF THE NATIONAL PARK SYSTEM § 7.1 Colonial National Historical Park....

  8. 10 CFR 71.103 - Quality assurance organization.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance organization. 71.103 Section 71.103... Quality Assurance § 71.103 Quality assurance organization. (a) The licensee, 2 certificate holder, and... location or locations where activities are performed, the organizational structure for executing...

  9. 46 CFR 71.40-1 - General or partial survey.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false General or partial survey. 71.40-1 Section 71.40-1... CERTIFICATION Inspection After Accident § 71.40-1 General or partial survey. (a) A survey, either general or... survey shall be such as to insure that the necessary repairs or renewals have been effectively made,...

  10. 10 CFR 71.73 - Hypothetical accident conditions.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Hypothetical accident conditions. 71.73 Section 71.73... Package, Special Form, and LSA-III Tests 2 § 71.73 Hypothetical accident conditions. (a) Test procedures. Evaluation for hypothetical accident conditions is to be based on sequential application of the...

  11. 27 CFR 71.95 - Responsibilities of administrative law judges.

    Science.gov (United States)

    2010-04-01

    ... administrative law judges. 71.95 Section 71.95 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURES AND PRACTICES RULES OF PRACTICE IN PERMIT PROCEEDINGS Administrative Law Judges § 71.95 Responsibilities of administrative law...

  12. 27 CFR 71.100 - Unavailability of administrative law judge.

    Science.gov (United States)

    2010-04-01

    ... administrative law judge. 71.100 Section 71.100 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURES AND PRACTICES RULES OF PRACTICE IN PERMIT PROCEEDINGS Administrative Law Judges § 71.100 Unavailability of administrative law judge. In...

  13. 42 CFR 71.11 - Bills of health.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Bills of health. 71.11 Section 71.11 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES QUARANTINE, INSPECTION, LICENSING FOREIGN QUARANTINE Measures at Foreign Ports § 71.11 Bills of health. A carrier at any foreign port clearing...

  14. 27 CFR 71.37 - Notice of contemplated action.

    Science.gov (United States)

    2010-04-01

    ... action. 71.37 Section 71.37 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... PROCEEDINGS Compliance and Settlement Informal Settlement § 71.37 Notice of contemplated action. Where the... officer. Where informal settlement is not reached promptly because of inaction of the permittee...

  15. 27 CFR 71.38 - Limitation on informal settlement.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Limitation on informal settlement. 71.38 Section 71.38 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... PROCEEDINGS Compliance and Settlement Informal Settlement § 71.38 Limitation on informal settlement. Where...

  16. Additions to the aquatic diptera (Chaoboridae, Chironomidae, Culicidae, Tabanidae, Tipulidae) fauna of the White River National Wildlife Refuge, Arkansas

    Science.gov (United States)

    Chordas, Stephen W., III; Hudson, Patrick L.; Chapman, Eric G.

    2004-01-01

    The dipteran fauna of Arkansas is generally poorly known. A previous study of the Aquatic macroinvertebrates of the White River National Wildlife Refuge, the largest refuge in Arkansas, reported only 12 diptera taxa out of 219 taxa collected (Chordas et al., 1996). Most of the dipterans from this study were identified only to the family level. The family Chironomidae is a large, diverse group and was predicted to be much more diverse in the refuge than indicated by previous studies. In this study, Chironomidae were targeted, with other aquatic or semiaquatic dipterans also retained, in collections designed to better define the dipteran fauna of the White River National Wildlife Refuge. Adult dipterans were collected from 22 sites within the refuge using sweep-nets, two types of blacklight traps, and lighted fan traps in June of 2001. Specimens from previous studies were retrieved and identified to the lowest possible taxonomic level. A total of 4,917 specimens representing 122 taxa was collected. The 122 taxa were comprised of the following: two chaoborids, 83 chironomids, 15 culicids, nine tabanids, and 13 tipulids. Of these, 46 species are new state records for Arkansas. Nine undescribed species of chironomids were collected, and eight species records represent significant range extensions.

  17. Phenotypic and genotypic characteristics of novel mouse cell line (NIH/3T3-adapted human enterovirus 71 strains (EV71:TLLm and EV71:TLLmv.

    Directory of Open Access Journals (Sweden)

    Carla Bianca Luena Victorio

    Full Text Available Since its identification in 1969, Enterovirus 71 (EV71 has been causing periodic outbreaks of infection in children worldwide and most prominently in the Asia-Pacific Region. Understanding the pathogenesis of Enterovirus 71 (EV71 is hampered by the virus's inability to infect small animals and replicate in their derived in vitro cultured cells. This manuscript describes the phenotypic and genotypic characteristics of two selected EV71 strains (EV71:TLLm and EV71:TLLmv, which have been adapted to replicate in mouse-derived NIH/3T3 cells, in contrast to the original parental virus which is only able to replicate in primate cell lines. The EV71:TLLm strain exhibited productive infection in all primate and rodent cell lines tested, while EV71:TLLmv exhibited greater preference for mouse cell lines. EV71:TLLmv displayed higher degree of adaptation and temperature adaptability in NIH/3T3 cells than in Vero cells, suggesting much higher fitness in NIH/3T3 cells. In comparison with the parental EV71:BS strain, the adapted strains accumulated multiple adaptive mutations in the genome resulting in amino acid substitutions, most notably in the capsid-encoding region (P1 and viral RNA-dependent RNA polymerase (3D. Two mutations, E167D and L169F, were mapped to the VP1 canyon that binds the SCARB2 receptor on host cells. Another two mutations, S135T and K140I, were located in the VP2 neutralization epitope spanning amino acids 136-150. This is the first report of human EV71 with the ability to productively infect rodent cell lines in vitro.

  18. Progress in the research of enterovirus 71%肠道病毒71型的研究进展

    Institute of Scientific and Technical Information of China (English)

    顾文珍

    2010-01-01

    Enterovirus 71 (EV71) is one of the main pathogens of hand, foot and mouth disease resulting in severe neurological complications and fatality. In fact, EV71 has been regarded as the most important neurotropic enterovirus after eradication of poliovirus. This paper reviews predisposing factors of EV71 infection and progress in the research of EV71 vaccine, thus pointing out the way for prevention and control of EV71 infection.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病(hand,foot and mouth disease,HFMD)的主要病原体之一,HFMD可并发严重神经系统疾病,甚至导致死亡.事实上,EV71一直被视为继脊髓灰质炎病毒消灭后最重要的嗜神经病毒.此文就EV71感染的易感因素以及疫苗的研究进展进行综述,从而为EV71感染的防控指明方向.

  19. Characteristics and treatment strategies of severe enterovirus 71 (EV71) infection in children%小儿危重肠病毒71型(EV71)感染临床特征及救治策略

    Institute of Scientific and Technical Information of China (English)

    张育才; 徐梁; 戎群芳

    2010-01-01

    @@ 肠病毒71型(Enterovirus 71,EV71)是手足口病病原之一,也是发生危重病例最重要的病原.根据中国卫生部及疾病预防和控制中心资料,绝大多数死亡病例是由E71感染所致.重症EV71感染主要表现为脑干脑炎、脑脊髓炎、脊髓灰质炎样综合征等.

  20. Survey of Borreliae in ticks, canines, and white-tailed deer from Arkansas, U.S.A.

    Directory of Open Access Journals (Sweden)

    Fryxell Rebecca T

    2012-07-01

    Full Text Available Abstract Background In the Eastern and Upper Midwestern regions of North America, Ixodes scapularis (L. is the most abundant tick species encountered by humans and the primary vector of B. burgdorferi, whereas in the southeastern region Amblyomma americanum (Say is the most abundant tick species encountered by humans but cannot transmit B. burgdorferi. Surveys of Borreliae in ticks have been conducted in the southeastern United States and often these surveys identify B. lonestari as the primary Borrelia species, surveys have not included Arkansas ticks, canines, or white-tailed deer and B. lonestari is not considered pathogenic. The objective of this study was to identify Borrelia species within Arkansas by screening ticks (n = 2123, canines (n = 173, and white-tailed deer (n = 228 to determine the identity and locations of Borreliae endemic to Arkansas using PCR amplification of the flagellin (flaB gene. Methods Field collected ticks from canines and from hunter-killed white-tailed were identified to species and life stage. After which, ticks and their hosts were screened for the presence of Borrelia using PCR to amplify the flaB gene. A subset of the positive samples was confirmed with bidirectional sequencing. Results In total 53 (21.2% white-tailed deer, ten (6% canines, and 583 (27.5% Ixodid ticks (252 Ixodes scapularis, 161 A. americanum, 88 Rhipicephalus sanguineus, 50 Amblyomma maculatum, 19 Dermacentor variabilis, and 13 unidentified Amblyomma species produced a Borrelia flaB amplicon. Of the positive ticks, 324 (22.7% were collected from canines (151 A. americanum, 78 R. sanguineus, 43 I. scapularis, 26 A. maculatum, 18 D. variabilis, and 8 Amblyomma species and 259 (37.2% were collected from white-tailed deer (209 I. scapularis, 24 A. maculatum, 10 A. americanum, 10 R. sanguineus, 1 D. variabilis, and 5 Amblyomma species. None of the larvae were PCR positive. A majority of the flaB amplicons were homologous with B

  1. Factors related to achievement in sophomore organic chemistry at the University of Arkansas

    Science.gov (United States)

    Lindsay, Harriet Arlene

    The purpose of this study was to identify the significant cognitive and non-cognitive variables that related to achievement in the first semester of organic chemistry at the University of Arkansas. Cognitive variables included second semester general chemistry grade, ACT composite score, ACT English, mathematics, reading, and science reasoning subscores, and spatial ability. Non-cognitive variables included anxiety, confidence, effectance motivation, and usefulness. Using a correlation research design, the individual relationships between organic chemistry achievement and each of the cognitive variables and non-cognitive variables were assessed. In addition, the relationships between organic chemistry achievement and combinations of these independent variables were explored. Finally, gender- and instructor-related differences in the relationships between organic chemistry achievement and the independent variables were investigated. The samples consisted of volunteers from the Fall 1999 and Fall 2000 sections of Organic Chemistry I at the University of Arkansas. All students in each section were asked to participate. Data for spatial ability and non-cognitive independent variables were collected using the Purdue Visualization of Rotations test and the modified Fennema-Sherman Attitude Scales. Data for other independent variables, including ACT scores and second semester general chemistry grades, were obtained from the Office of Institutional Research. The dependent variable, organic chemistry achievement, was measured by each student's accumulated points in the course and consisted of scores on quizzes and exams in the lecture section only. These totals were obtained from the lecture instructor at the end of each semester. Pearson correlation and stepwise multiple regression analyses were used to measure the relationships between organic chemistry achievement and the independent variables. Prior performance in chemistry as measured by second semester general

  2. Using Paid Radio Advertisements to Promote Physical Activity Among Arkansas Tweens

    Directory of Open Access Journals (Sweden)

    Appathurai Balamurugan, MD, MPH

    2005-11-01

    Full Text Available Introduction The level of physical activity among children is a growing concern. Evidence shows that many children aged 9 to 13 years (tweens do not participate in any organized physical activity during their nonschool hours, and some do not engage in any free-time physical activity. Physical inactivity is associated with a host of chronic diseases such as diabetes and cardiovascular disease. Paid media advertisements have been an effective method of promoting physical activity. Methods From March 10, 2003, through June 29, 2003, we aired paid radio advertisements in six major Arkansas metropolitan areas to promote physical activity among tweens. In September 2003, we surveyed 295 Arkansas tweens by telephone to assess their exposure to the advertisements and the impact of the advertisements on their intent to participate in physical activity. In the same telephone survey, we also asked questions about the respondents’ physical activity level. The data were weighted so that the results would be representative of the areas surveyed. The statistical analysis was performed using SPSS, version 11.5 (SPSS Inc, Chicago, Ill. Results Of the tweens surveyed, 56.4% (95% confidence interval [CI], 50.7%–62.1% reported hearing the radio advertisements. Of the tweens who heard the advertisement messages, 76.1% (95% CI, 69.4%–82.8% said the messages made them more likely to get involved in physical activity. Younger tweens (aged 9 and 10 years were less likely to have heard the advertisements than older tweens (aged 11 to 13 years. However, the advertisements were more likely to cause younger tweens to want to get involved in physical activity (odds ratio [OR] = 6.89, P = .003 than older tweens. Of the tweens surveyed, 74.9% (95% CI, 70.0%–79.8% reported that they were involved in nonschool-sponsored sports, and 45.3% (95% CI, 39.6%–51.0% were involved in school-sponsored sports. Conclusion Paid media advertisements may be an effective way to

  3. The Construction of the EGFP Enterovirus 71%肠道病毒71型(EV71)荧光病毒的构建

    Institute of Scientific and Technical Information of China (English)

    朱守海; 王璐璐

    2014-01-01

    人肠道病毒71型(Enterovirus,EV71)是引起手足口病的主要病原体。首先利用反向遗传学的方法,构建了EV71全长cDNA感染性克隆,经体外转录、转染RD细胞后成功获得了拯救病毒。随后,将增强型绿色荧光蛋白基因(Enhanced green fluorescent protein,EGFP)插入到EV71基因组中构建荧光病毒。结果表明,此荧光病毒不仅可以侵染、复制,在传代的过程中EGFP基因也保持了较好的稳定性,表明其可以作为一种报告病毒应用于高通量的EV71抗病毒药物筛选中。%Human Enterovirus 71(EV71)is one of the major agents causing hand, foot and mouth disease(HFMD). An infectious full-length cDNA clone of EV71 as well as the recuse virus were obtained using the reverse genetic techniques. Then, the EGFP gene was inserted into the EV71 genome to construct the reporter virus. The results show that the EGFP-EV71 virus not only are replication-competent and infectious, but the EGFP gene are stable during passaging, implicating the recombinant virus could be used as a reporter virus for high throughput EV71 antiviral drugs screening.

  4. 肠道病毒71型疫苗研究进展%Research progress of enterovirus 71 vaccines

    Institute of Scientific and Technical Information of China (English)

    袁晓晶; 温红玲; 王志玉

    2012-01-01

    Enterovirus 71 (EV71) is one of the main causative pathogens of hand,foot and mouth disease resulting in neurological complications and death.There are no effective drugs and measures for EV71 infection at present.Development of effective vaccines is the best method to control EV71 epidemic.This review summarizes the research progress of EV71 vaccines.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病(hand,foot and mouth disease,HFMD)的主要病原体之一,HFMD可并发神经系统疾病,甚至导致死亡.对于EV71感染,目前尚无有效的治疗药物和措施,因此研制有效的疫苗是控制EV71流行的最佳方法.此文就EV71疫苗的研究进展进行综述.

  5. Cadmium Accumulation in Periphyton from an Abandoned Mining District in the Buffalo National River, Arkansas.

    Science.gov (United States)

    McCauley, Jacob R; Bouldin, Jennifer L

    2016-06-01

    The Rush Mining District along the Buffalo River in Arkansas has a significant history of zinc and lead mining operations. The tails and spoils of these operations deposit heavy amounts of raw ore into streams. One element commonly found in the earth's crust that becomes a minor constituent of the deposition is cadmium. Periphyton samples from Rush Creek and Clabber Creek, two creeks within the Rush Mining District were measured for cadmium as well as two creeks with no history of mining, Spring Creek and Water Creek. Periphyton samples from Rush and Clabber Creek contained mean cadmium concentrations of 436.6 ± 67.3 and 93.38 ± 8.67 µg/kg, respectively. Spring Creek and Water Creek had a mean cadmium concentration of 40.49 ± 3.40 and 41.78 ± 3.99 µg/kg within periphyton. The results indicate increased metal concentrations in algal communities from mined areas. As periphyton is the base of the aquatic food chain, it acts as a conduit for movement of cadmium in the food web. PMID:27130541

  6. Digital model of the Bayou Bartholomew alluvial aquifer stream system, Arkansas

    Science.gov (United States)

    Reed, J.E.; Broom, Matthew E.

    1979-01-01

    A digital model of the Bayou Bartholomew aquifer-stream system in Arkansas was calibrated for the purpose of predicting hydrologic responses to stresses of water development. The simulated-time span for model calibration was from 1953 to 1970, during which time the system was stressed largely by ground- and surface-water diversions for rice irrigation. The model was calibrated by comparing groundwater-level and streamflow data with model-derived groundwater levels and streamflow. In the calibrated model, the ratio of model-derived to observed streamflows for 17 subbasins averaged 1.1; the ratios among the subbasins ranged from 0.8 to 1.6. The average deviation of the differences between model-derived and observed groundwater levels at 47 nodes was 0.2; the average among the nodes ranged from -2.3 to 10.4. The average standard deviation of the differences between the model-derived and observed groundwater levels was 3.5; the average among the nodes ranged from 0.4 to 10.5. The model will provide projections of changes in the potentiometric surface resulting from (1) changes in the rate or distribution of groundwater pumpage or (2) changes in the stage of streams and reservoirs. The model will provide only approximate projections of the streamflow. (USGS)

  7. Atmospheric Contributors to Heavy Rainfall Events in the Arkansas-Red River Basin

    Directory of Open Access Journals (Sweden)

    Taylor A. McCorkle

    2016-01-01

    Full Text Available This study analyzed the top 1% 24-hour rainfall events from 1994 to 2013 at eight climatological sites that represent the east to west precipitation gradient across the Arkansas-Red River Basin in North America. A total of 131 cases were identified and subsequently classified on the synoptic-scale, mesoscale, and local-scale to compile a climatological analysis of these extreme, heavy rainfall events based on atmospheric forcings. For each location, the prominent midtropospheric pattern, mesoscale feature, and predetermined thermodynamic variables were used to classify each 1% rainfall event. Individual events were then compared with other cases throughout the basin. The most profound results were that the magnitudes of the thermodynamic variables such as convective available potential energy and precipitable water values were poor predictors of the amount of rainfall produced in these extreme events. Further, the mesoscale forcings had more of an impact during the warm season and for the westernmost locations, whereas synoptic forcings were extremely prevalent during the cold season at the easternmost locations in the basin. The implications of this research are aimed at improving the forecasting of heavy precipitation at individual weather forecasts offices within the basin through the identified patterns at various scales.

  8. Disposal of chemical agents and munitions stored at Pine Bluff Arsenal, Pine Bluff, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Ensminger, J.T.; Hillsman, E.L.; Johnson, R.D.; Morrisey, J.A.; Staub, W.P.; Boston, C.R.; Hunsaker, D.B.; Leibsch, E.; Rickert, L.W.; Tolbert, V.R.; Zimmerman, G.P.

    1991-09-01

    The Pine Bluff Arsenal (PBA) near Pine Bluff, Arkansas, is one of eight continental United States (CONUS) Army installations where lethal unitary chemical agents and munitions are stored and where destruction of agents and munitions is proposed under the Chemical Stockpile Disposal Program (CSDP). The chemical agent inventory at PBA consists of approximately 12%, by weight, of the total US stockpile. The destruction of the stockpile is necessary to eliminate the risk to the public from continued storage and to dispose of obsolete and leaking munitions. In 1988 the US Army issued a Final Programmatic Environmental Impact Statement (FPEIS) for the CSDP that identified on-site disposal of agents and munitions as the environmentally preferred alternative (i.e., the alternative with the least potential to cause significant adverse impacts). The purpose of this report is to examine the proposed implementation of on-site disposal at PBA in light of more recent and more detailed data than those on which the FPEIS is based. New population data were used to compute fatalities using the same computation methods and values for all other parameters as in the FPEIS. Results indicate that all alternatives are indistinguishable when the potential health impacts to the PBA community are considered. However, risks from on-site disposal are in all cases equal to or less than risks from other alternatives. Furthermore, no unique resources with the potential to prevent or delay implementation of on-site disposal at PBA have been identified.

  9. Size distribution of planktonic autotrophy and microheterotrophy in DeGray Reservoir, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Kimmel, B.L.; Groeger, A.W.

    1983-01-01

    Naturally occurring assemblages of phytoplankton and bacterioplankton were radiolabelled with sodium /sup 14/C-bicarbonate and sodium /sup 3/H-acetate and size fractionated to determine the size structure of planktonic autotrophy and microheterotrophy in DeGray Reservoir, an oligotrophic impoundment of the Caddo River in south-central Arkansas. Size distributions of autotrophy and microheterotrophy were remarkably uniform seasonally, vertically within the water column, and along the longitudinal axis of the reservoir despite significant changes in environmental conditions. Planktonic autotrophy was dominated by small algal cells with usually >50% of the photosynthetic carbon uptake accounted for by organisms <8.0 ..mu..m. Microheterotrophic activity in the 0.2- to 1.0-..mu..m size fraction, presumably associated with free-living bacterioplankton not attached to suspended particles, usually accounted for >75% of the planktonic microheterotrophy. Longitudinal patterns in autotrophic and microheterotrophic activities associated with >3-..mu..m and >1-..mu..m size fractions, respectively, suggest an uplake to downlake shift from riverine to lacustrine environmental influences within the reservoir. 83 references, 7 figures.

  10. Predictors of Substance Abuse Treatment Entry Among Rural Illicit Stimulant Users in Ohio, Arkansas, and Kentucky

    Science.gov (United States)

    Carlson, Robert G.; Sexton, Rocky; Wang, Jichuan; Falck, Russel; Leukefeld, Carl G.; Booth, Brenda M.

    2010-01-01

    Illicit drug use in the rural United States is increasingly common, yet little is known about drug users’ treatment-seeking behaviors. This study identifies predictors of substance abuse treatment entry over 24 months among 710 illicit stimulant users in rural areas of Ohio, Arkansas, and Kentucky. Active users of powdered cocaine, crack cocaine, and/or methamphetamine (MA) were recruited using respondent-driven sampling. Participants completed structured interviews at baseline and follow-up questionnaires every 6 months for 24 months. Data were analyzed using the Cox proportional hazards model. The paper is informed by the Anderson-Newman Model. Overall, 18.7% of the sample entered treatment. Ohio or Kentucky residence, perceived need for substance abuse treatment, higher ASI legal problem composite scores, prior substance abuse treatment, and tranquilizer use were positively associated with treatment entry. Non-daily crack cocaine users and marijuana users were less likely to enter treatment. The findings can help inform rural substance abuse treatment program development and outreach. PMID:20391264

  11. Description of Survey Data Regarding the Chemical Repackaging Plant Accident West Helena, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Sorensen, J.H.; Vogt, B.M.

    1999-03-01

    Shortly after 1:00 p.m. on Thursday, May 8, 1997, clouds of foul-smelling smoke began pouring from an herbicide and pesticide packaging plant in West Helena, Arkansas. An alert was sounded, employees evacuated, and the West Helena fire department was called. As three firefighters prepared to enter the plant, the chemical compounds exploded, collapsing a solid concrete block wall, and killing all three firefighters. As the odorous smoky cloud drifted away from the plant, authorities ordered residents in a 2-mile area downwind of the plant to evacuate and those in the 2- to 3-mile zone to shelter in place. This study examines and compares the responses to a mail survey of those ordered to evacuate and those told to shelter in place. Among the variables examined are compliance with official orders and perceived warnings, threat perception, time and source of first warning, response times, and behavior characteristics for both populations. The findings indicate that 90% of those that were told to evacuate did so but only 27% of those told to shelter-in-place did so, with 68% opting to evacuate instead. The implications of these findings for emergency managers is that people will likely choose to evacuate when both warnings to evacuate and warnings to shelter are issued to residents in close proximity to each other. The findings on warning times closely resemble other findings from evacuations when chemical accidents occur and route notification is used for warning residents.

  12. Duplex-like structures in submarine fan channels, Ouachita Mountains, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Shanmugam, G.; Moiola, R.J.; Sales, J.K.

    1988-03-01

    Submarine fan channel sequences of the Jackfork Formation (Lower Pennsylvanian) at DeGray Dam section in the Ouachita Mountains, Arkansas, contain discrete units (10-75 cm thick) with moderately dipping (25/sup 0/-40/sup 0/), sigmoidal imbricate slices. Adjacent units with opposing imbricate slices are common. The sigmoidal structures are similar in geometry to a tectonic feature known as a duplex. A tectonic origin of sigmoidal structures, however, seems unlikely because opposing directions of imbrication in adjacent units would require an unrealistic tectonic movement history for the area. The authors propose that the Jackfork sigmoidal structures were formed by a process kinematically similar to that responsible for generating duplex structures. Unlike tectonic duplexes, however, the sigmoidal structures were formed by soft-sediment deformation of sand and mud layers as high-energy sediment gravity flows glided over these layers. Sediment gravity flows, responsible for forming the sigmoidal deformation, were probably generated by slumping of adjacent channel walls. Dip direction of sigmoidal slices is perpendicular to channel axes. Thus, recognition of sigmoidal deformation structures may be useful in inferring the trend of channels in ancient submarine fan complexes.

  13. Burnup verification measurements on spent fuel assemblies at Arkansas Nuclear One

    International Nuclear Information System (INIS)

    Burnup verification measurements have been performed using the Fork system at Arkansas Nuclear One, Units 1 and 2, operated by Energy Operations, Inc. Passive neutron and gamma-ray measurements on individual spent fuel assemblies were correlated with the reactor records for burnup, cooling time, and initial enrichment. The correlation generates an internal calibration for the system in the form of a power law determined by a least squares fit to the neutron data. The values of the exponent in the power laws were 3.83 and 4.35 for Units 1 and 2, respectively. The average deviation of the reactor burnup records from the calibration determined from the measurements is a measure of the random error in the burnup records. The observed average deviations were 2.7% and 3.5% for assemblies at Units 1 and 2, respectively, indicating a high degree of consistency in the reactor records. Two non-standard assemblies containing neutron sources were studied at Unit 2. No anomalous measurements were observed among the standard assemblies at either Unit. The effectiveness of the Fork system for verification of reactor records is due to the sensitivity of the neutron yield to burnup, the self-calibration generated by a series of measurements, the redundancy provided by three independent detection systems, and the operational simplicity and flexibility of the design

  14. The UP modelling system for large scale hydrology: simulation of the Arkansas-Red River basin

    Directory of Open Access Journals (Sweden)

    C. G. Kilsby

    1999-01-01

    Full Text Available The UP (Upscaled Physically-based hydrological modelling system to the Arkansas-Red River basin (USA is designed for macro-scale simulations of land surface processes, and aims for a physical basis and, avoids the use of discharge records in the direct calibration of parameters. This is achieved in a two stage process: in the first stage parametrizations are derived from detailed modelling of selected representative small and then used in a second stage in which a simple distributed model is used to simulate the dynamic behaviour of the whole basin. The first stage of the process is described in a companion paper (Ewen et al., this issue, and the second stage of this process is described here. The model operated at an hourly time-step on 17-km grid squares for a two year simulation period, and represents all the important hydrological processes including regional aquifer recharge, groundwater discharge, infiltration- and saturation-excess runoff, evapotranspiration, snowmelt, overland and channel flow. Outputs from the model are discussed, and include river discharge at gauging stations and space-time fields of evaporation and soil moisture. Whilst the model efficiency assessed by comparison of simulated and observed discharge records is not as good as could be achieved with a model calibrated against discharge, there are considerable advantages in retaining a physical basis in applications to ungauged river basins and assessments of impacts of land use or climate change.

  15. Modeling regional variation in riverine fish biodiversity in the Arkansas-White-Red River basin

    Energy Technology Data Exchange (ETDEWEB)

    Schweizer, Peter E [ORNL; Jager, Yetta [ORNL

    2011-01-01

    The patterns of biodiversity in freshwater systems are shaped by biogeography, environmental gradients, and human-induced factors. In this study, we developed empirical models to explain fish species richness in subbasins of the Arkansas White Red River basin as a function of discharge, elevation, climate, land cover, water quality, dams, and longitudinal position. We used information-theoretic criteria to compare generalized linear mixed models and identified well-supported models. Subbasin attributes that were retained as predictors included discharge, elevation, number of downstream dams, percent forest, percent shrubland, nitrate, total phosphorus, and sediment. The random component of our models, which assumed a negative binomial distribution, included spatial correlation within larger river basins and overdispersed residual variance. This study differs from previous biodiversity modeling efforts in several ways. First, obtaining likelihoods for negative binomial mixed models, and thereby avoiding reliance on quasi-likelihoods, has only recently become practical. We found the ranking of models based on these likelihood estimates to be more believable than that produced using quasi-likelihoods. Second, because we had access to a regional-scale watershed model for this river basin, we were able to include model-estimated water quality attributes as predictors. Thus, the resulting models have potential value as tools with which to evaluate the benefits of water quality improvements to fish.

  16. Bacteria and Turbidity Survey for Blue Mountain Lake, Arkansas, Spring and Summer, 1994

    Science.gov (United States)

    Lasker, A. Dwight

    1995-01-01

    Introduction Blue Mountain Lake darn is located at river mile 74.4 on the Petit Jean River in Logan and Yell Counties in west-central Arkansas (fig. 1). Drainage area above the darn is 488 square miles. Blue Mountain Lake is located between two national forests-the Ozark National Forest and the Ouachita National Forest. The primary purpose for Blue Mountain Lake is flood control, but the lake is used for a variety of recreational purposes. The U.S. Geological Survey (USGS) in cooperation with the U.s. Army Corps of Engineers, Little Rock District, conducted a bacterial and turbidity study of the Blue Mountain Lake Basin during the spring and suri1mer 1994. Samples were collected weekly at 11 locations within the lake basin from May through September 1994. Eight sampling sites were located on tributaries to the lake and three sampling sites were located on the lake with one of the sites located at a swim beach (fig. 2; table 1).

  17. Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry.

    Science.gov (United States)

    Xu, Fengwen; Zhao, Xiaoxiao; Hu, Siqi; Li, Jian; Yin, Lijuan; Mei, Shan; Liu, Tingting; Wang, Ying; Ren, Lili; Cen, Shan; Zhao, Zhendong; Wang, Jianwei; Jin, Qi; Liang, Chen; Ai, Bin; Guo, Fei

    2016-01-01

    Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection. PMID:27608771

  18. Norepinephrine and Epinephrine Enhanced the Infectivity of Enterovirus 71.

    Directory of Open Access Journals (Sweden)

    Yu-Ting Liao

    Full Text Available Enterovirus 71 (EV71 infections may be associated with neurological complications, including brainstem encephalitis (BE. Severe EV71 BE may be complicated with autonomic nervous system (ANS dysregulation and/or pulmonary edema (PE. ANS dysregulation is related to the overactivation of the sympathetic nervous system, which results from catecholamine release.The aims of this study were to explore the effects of catecholamines on severe EV71 infection and to investigate the changes in the percentages of EV71-infected cells, virus titer, and cytokine production on the involvement of catecholamines.Plasma levels of norepinephrine (NE and epinephrine (EP in EV71-infected patients were measured using an enzyme-linked immunoassay. The expression of adrenergic receptors (ADRs on RD, A549, SK-N-SH, THP-1, Jurkat and human peripheral blood mononuclear cells (hPBMCs were detected using flow cytometry. The percentages of EV71-infected cells, virus titer, and cytokine production were investigated after treatment with NE and EP.The plasma levels of NE and EP were significantly higher in EV71-infected patients with ANS dysregulation and PE than in controls. Both α1A- and β2-ADRs were expressed on A549, RD, SK-N-SH, HL-60, THP-1, Jurkat cells and hPBMCs. NE treatment elevated the percentages of EV71-infected cells to 62.9% and 22.7% in THP-1 and Jurkat cells, respectively. Via treatment with EP, the percentages of EV71-infected cells were increased to 64.6% and 26.9% in THP-1 and Jurkat cells. The percentage of EV71-infected cells increased upon NE or EP treatment while the α- and β-blockers reduced the percentages of EV71-infected cells with NE or EP treatment. At least two-fold increase in virus titer was observed in EV71-infected A549, SK-N-SH and hPBMCs after treatment with NE or EP. IL-6 production was enhanced in EV71-infected hPBMCs at a concentration of 102 pg/mL NE.The plasma levels of NE and EP elevated in EV71-infected patients with ANS

  19. Radiotherapy for 71 patients with tonsillar carcinoma

    International Nuclear Information System (INIS)

    Objective: To evaluate the therapeutic outcome and determine the prognosticators of tonsillar carcinoma treated by different approaches. Methods: A retrospective study was done on 71 tonsillar carcinoma patients treated from August 1971 to October 1995 by three different ways of radiotherapy. All were pathologically proved. Fifteen patients received external bean therapy plus intracavitary brachytherapy (EBRT + BRT), 39 patients were treated by EBRT only (EBRT) and the other 17 patients underwent FBRT plus chemotherapy (EBTR + CHEMO). Results: The local control rates of EBRT + BRT, EBRT and EBRT + chemo were 82.1%, 68.4% and 64.2%. There was significant difference between EBRT + BRT and the other two groups (x2=4.65, P=0.030; x2=5.65, P=0.018). The 3-, 5- and 10- year survival rates of EBRT + BRT, EBRT and EBRT + CHEMO were 76.3%, 53.2%, 31.2%; 74.0%, 45.2%, 33.5% and 75.2%, 47.1%, 25.3%. All these survival rates showed no statistical significance. (P > 0.05). Conclusion: Brachytherapy, when used to increase the dose effect, may enhance the local effect without any change in the overall survival

  20. Subtask 7.1 - Strategic Studies

    Energy Technology Data Exchange (ETDEWEB)

    Thomas Erickson

    2009-03-30

    The Energy & Environmental Research Center (EERC) has recently completed 11 years of research through the Cooperative Agreement with the U.S. Department of Energy (DOE) National Energy Technology Laboratory (NETL) focused on fossil energy technology development and demonstration. To support a significant number of the different activities being considered within all of our research contracts with NETL, a subtask (7.1 Strategic Studies) was created to focus on small research efforts that came up throughout the year which would support an existing EERC-NETL project or would help to develop a new concept for inclusion in future efforts. Typical efforts conducted under this task were usually between $15,000 and $60,000 in scope and had time lines of less than 6 months. A limited number of larger studies were also conducted, generally at the direct request of NETL. Over the life of this task, 46 projects were conducted. These efforts ranged from quick experiments to gain fundamental knowledge to support a current effort, to literature reviews, to a few larger engineering efforts.

  1. Epidemiology and seroepidemiology of human enterovirus 71 among Thai populations

    OpenAIRE

    Linsuwanon, Piyada; Puenpa, Jiratchaya; Huang, Sheng-Wen; Wang, Ya-Fang; Mauleekoonphairoj, John; Wang, Jen-Ren; Poovorawan, Yong

    2014-01-01

    Background Human enterovirus 71 (EV71) is an important pathogen caused large outbreaks in Asian-Pacific region with severe neurological complications and may lead to death in young children. Understanding of the etiological spectrum and epidemic changes of enterovirus and population’s immunity against EV71 are crucial for the implementation of future therapeutic and prophylactic intervention. Results A total of 1,182 patients who presented with the symptoms of hand foot and mouth disease (67....

  2. Human SCARB2-mediated entry and endocytosis of EV71.

    Directory of Open Access Journals (Sweden)

    Yi-Wen Lin

    Full Text Available Enterovirus (EV 71 infection is known to cause hand-foot-and-mouth disease (HFMD and in severe cases, induces neurological disorders culminating in fatality. An outbreak of EV71 in South East Asia in 1997 affected over 120,000 people and caused neurological disorders in a few individuals. The control of EV71 infection through public health interventions remains minimal and treatments are only symptomatic. Recently, human scavenger receptor class B, member 2 (SCARB2 has been reported to be a cellular receptor of EV71. We expressed human SCARB2 gene in NIH3T3 cells (3T3-SCARB2 to study the mechanisms of EV71 entry and infection. We demonstrated that human SCARB2 serves as a cellular receptor for EV71 entry. Disruption of expression of SCARB2 using siRNAs can interfere EV71 infection and subsequent inhibit the expression of viral capsid proteins in RD and 3T3-SCARB2 but not Vero cells. SiRNAs specific to clathrin or dynamin or chemical inhibitor of clathrin-mediated endocytosis were all capable of interfering with the entry of EV71 into 3T3-SCARB2 cells. On the other hand, caveolin specific siRNA or inhibitors of caveolae-mediated endocytosis had no effect, confirming that only clathrin-mediated pathway was involved in EV71 infection. Endocytosis of EV71 was also found to be pH-dependent requiring endosomal acidification and also required intact membrane cholesterol. In summary, the mechanism of EV71 entry through SCARB2 as the receptor for attachment, and its cellular entry is through a clathrin-mediated and pH-dependent endocytic pathway. This study on the receptor and endocytic mechanisms of EV71 infection is useful for the development of effective medications and prophylactic treatment against the enterovirus.

  3. Development of EV71 virus-like particle purification processes.

    Science.gov (United States)

    Lin, Shih-Yeh; Chiu, Hsin-Yi; Chiang, Bor-Luen; Hu, Yu-Chen

    2015-11-01

    Enterovirus 71 (EV71) causes the outbreaks of hand-foot-and-mouth disease and results in deaths of hundreds of young children. EV71 virus-like particles (VLPs) are empty capsids consisting of viral structural proteins and can elicit potent immune responses, thus holding promise as an EV71 vaccine candidate. However, an efficient, scalable production and purification scheme is missing. For mass production of EV71 VLPs, this study aimed to develop a production and chromatography-based purification process. We first demonstrated the successful EV71 VLPs production in the stirred-tank bioreactor in which High Five™ cells were infected with a recombinant baculovirus co-expressing EV71 structural polyprotein P1 and protease 3CD. The culture supernatant containing the VLPs was subjected to tangential flow filtration (TFF) for concentration/diafiltration, which enabled the removal of >80% of proteins while recovering >80% of VLPs. The concentrated VLPs were next subjected to hydroxyapatite chromatography (HAC) in which the VLPs were mainly found in the flow through. After another TFF concentration/diafiltration, the VLPs were purified by size-exclusion chromatography (SEC) and concentrated/diafiltered by a final TFF. The integrated process yielded an overall VLPs recovery of ≈ 36% and a purity of ≈ 83%, which was better or comparable to the recovery and purity for the purification of live EV71 virus particles. This process thus may move the EV71 VLPs vaccine one step closer to the clinical applications. PMID:25939279

  4. Considerations for developing an immunization strategy with enterovirus 71 vaccine.

    Science.gov (United States)

    Li, Li; Yin, Hongzhang; An, Zhijie; Feng, Zijian

    2015-02-25

    Enterovirus 71 (EV71) is a common pathogen for hand, foot, and mouth disease (HFMD), which has significant morbidity and mortality, and for which children aged 6-59 months age are at highest risk. Due to lack of effective treatment options, control of EV71 epidemics has mainly focused on development of EV71 vaccines. Clinical trials have been completed on 3 EV71 vaccines, with trial results demonstrating good vaccine efficacy and safety. When EV71 vaccine is approved by China's national regulatory authority, an evidence-based strategy should be developed to optimize impact and safety. An immunization strategy for EV71 vaccine should consider several factors, including the target population age group, the number of doses for primary immunization, the need for a booster dose, concomitant administration of other vaccines, economic value, program capacity and logistics, and public acceptance. Once EV71 vaccines are in use, vaccine effectiveness and safety must be monitored in large populations, and the epidemiology of HFMD must be evaluated to assure a match between vaccination strategy and epidemiology. Evaluation in China is especially important because there are no other EV71 vaccines globally. PMID:25444807

  5. Animal models of enterovirus 71 infection: applications and limitations

    OpenAIRE

    Wang, Ya-Fang; Yu, Chun-Keung

    2014-01-01

    Human enterovirus 71 (EV71) has emerged as a neuroinvasive virus that is responsible for several outbreaks in the Asia-Pacific region over the past 15 years. Appropriate animal models are needed to understand EV71 neuropathogenesis better and to facilitate the development of effective vaccines and drugs. Non-human primate models have been used to characterize and evaluate the neurovirulence of EV71 after the early outbreaks in late 1990s. However, these models were not suitable for assessing ...

  6. 肠道病毒71型研究进展%STUDY ON THE PROGRESS OF ENTEROVIRUS 71

    Institute of Scientific and Technical Information of China (English)

    孙佳

    2011-01-01

    Hand, foot and mouth disease is an acute infectious disease caused by different enteroviruses. including the coxsackievirus A16 and hu-man enterovirus 71 etc. Since EV71 can cause severe central nervous system complications. This article reviewed the research progress on EV 71 , provided the basis for the prevention and treatment of HFMD.%手足口病是由不同的肠道病毒所引起的一种急性传染病,其中以柯萨奇病毒A16型和人肠道病毒EV71最为常见.由于EV71能引起严重的中枢神经系统并发症,本文就EV71的研究进展作一综述,为手足口病的预防和治疗提供依据.

  7. Anomalous isobaric analogue resonance in 71As

    International Nuclear Information System (INIS)

    In 1971 G. M. Temmer et al., reported observing five anomalous substructures in a spin 1/2+ isobaric analogue resonance in 71As at E/sub p/ = 5.05 MeV. They suggested that these substructures could be interpreted as ''hallway states'' coupling a ''doorway state'' to compound nuclear states. Although doorway states are well established interpretations of isobaric analogue resonances, hallway states have no such acreditation. The case investigated in this paper was the first reported evidence suggesting the existence of hallway states in an isobaric analogue resonance. In this work the validity of the hallway state interpretation was ascertained for two of the five substructures. The spin and parity of these two substructures were determined to be 5/2+; therefore, their interpretation as hallway states is incorrect since it would not conserve angular momentum. The spins and parities of all the substructures were determined by measuring the analyzing power of the elastic reaction 70Ge(p(pol),p0) from an energy E/sub p/ = 4.92 MeV to an energy E/sub p/ = 5.23 MeV in 10 keV steps. The analyzing power was then remeasured over the last two substructures at E = 5.05 MeV and E = 5.14 MeV in 5 keV steps. These last two substructures also decayed strongly to the 2+ first excited state in 70Ge. The angular correlation between the first inelastic proton and the subsequently emitted gamma ray was measured in the Goldfarb--Seyler geometry and was then expanded in terms of cosine functions. The expansion coefficients implied that the spins of these two resonances were greater than or equal to 5/2, in agreement with the analyzing power measurements

  8. Aerial gamma ray and magnetic survey: Mississippi and Florida airborne survey, Fort Smith quadrangle, Oklahoma, and Arkansas. Final report

    International Nuclear Information System (INIS)

    The Fort Smith quadrangle in western Arkansas and eastern Oklahoma overlies thick Paleozoic sediments of the Arkoma Basin. These Paleozoics dominate surface exposure except where covered by Quaternary Alluvial materials. Examination of available literature shows no known uranium deposits (or occurrences) within the quadrangle. Seventy-five groups of uranium samples were defined as anomalies and are discussed briefly. None were considered significant, and most appeared to be of cultural origin. Magnetic data show character that suggest structural and/or lithologic complexity, but imply relatively deep-seated sources

  9. The interplays between autophagy and apoptosis induced by enterovirus 71.

    Directory of Open Access Journals (Sweden)

    Xueyan Xi

    Full Text Available BACKGROUND: Enterovirus 71 (EV71 is the causative agent of human diseases with distinct severity, from mild hand, foot and mouth disease to severe neurological syndromes, such as encephalitis and meningitis. The lack of understanding of viral pathogenesis as well as lack of efficient vaccine and drugs against this virus impedes the control of EV71 infection. EV71 virus induces autophagy and apoptosis; however, the relationship between EV71-induced autophagy and apoptosis as well as the influence of autophagy and apoptosis on virus virulence remains unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this study, it was observed that the Anhui strain of EV71 induced autophagy and apoptosis in human rhabdomyosarcoma (RD-A cells. Additionally, by either applying chemical inhibitors or knocking down single essential autophagic or apoptotic genes, inhibition of EV71 induced autophagy inhibited the apoptosis both at the autophagosome formation stage and autophagy execution stage. However, inhibition of autophagy at the stage of autophagosome and lysosome fusion promoted apoptosis. In reverse, the inhibition of EV71-induced apoptosis contributed to the conversion of microtubule-associated protein 1 light chain 3-I (LC3-I to LC3-II and degradation of sequestosome 1 (SQSTM1/P62. Furthermore, the inhibition of autophagy in the autophagsome formation stage or apoptosis decreased the release of EV71 viral particles. CONCLUSIONS/SIGNIFICANCE: In conclusion, the results of this study not only revealed novel aspect of the interplay between autophagy and apoptosis in EV71 infection, but also provided a new insight to control EV71 infection.

  10. Geologic Map of the Boxley Quadrangle, Newton and Madison Counties, Arkansas

    Science.gov (United States)

    Hudson, Mark R.; Turner, Kenzie J.

    2007-01-01

    This map summarizes the geology of the Boxley 7.5-minute quadrangle in the Ozark Plateaus region of northern Arkansas. Geologically, the area lies on the southern flank of the Ozark dome, an uplift that exposes oldest rocks at its center in Missouri. Physiographically, the Boxley quadrangle lies within the Boston Mountains, a high plateau region underlain by Pennsylvanian sandstones and shales. Valleys of the Buffalo River and its tributaries expose an approximately 1,600-ft-(490-m-)thick sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. Part of Buffalo National River, a park encompassing the Buffalo River and adjacent land that is administered by the National Park Service, extends through the eastern part of the quadrangle. Mapping for this study was conducted by field inspection of numerous sites and was compiled as a 1:24,000-scale geographic information system (GIS) database. Locations and elevation sites were determined with the aid of a global positioning satellite receiver and a hand-held barometric altimeter. Hill-shade-relief and slope maps derived from a U.S. Geological Survey 10-m digital elevation model as well as orthophotos were used to help trace ledge-forming units between field traverses within the Upper Mississippian and Pennsylvanian part of the stratigraphic sequence. Strike and dip of beds were typically measured along stream drainages or at well-exposed ledges. Structure contours were constructed on the top of the Boone Formation and the base of a prominent sandstone unit within the Bloyd Formation based on elevations of control points as well as other limiting information on their maximum or minimum elevations.

  11. Geomorphic Characterization of the Middle Fork Saline River: Garland, Perry, and Saline Counties, Arkansas

    Science.gov (United States)

    Pugh, Aaron L.; Garday, Thomas J.; Redman, Ronald

    2008-01-01

    This report was prepared to help address concerns raised by local residents, State, and Federal agencies about the current geomorphic conditions of the Middle Fork Saline River. Over the past 30 years the Middle Fork Saline River Basin has experienced a marked increase in urbanization. The report summarizes the Middle Fork?s current (2003) channel characteristics at nine stream reaches in the upper 91 square miles of the basin. Assessments at each study reach included comparing measured stream geometry dimensions (cross-sectional area, top width, and mean depth) at bankfull stage to regional hydraulic geometry curves for the Ouachita Mountains Physiographic Province of Arkansas and Oklahoma, evaluations of streambed materials and sinuosity, and classification of individual stream reach types. When compared to the Ouachita Mountains? regional hydraulic geometry curves for natural, stable, stream reaches, five of the nine study reaches had slightly smaller crosssectional areas, longer top widths, and shallower depths. Streambed material analysis indicates that the Middle Fork is a bedrock influenced, gravel dominated stream with lesser amounts of sand and cobbles. Slight increases in sinuosity from 1992 to 2002 at seven of the nine study reaches indicate a slight decrease in stream channel slope. Analyses of the Middle Fork?s hydraulic geometry and sinuosity indicate that the Middle Fork is currently overly wide and shallow, but is slowly adjusting towards a deeper, narrower hydraulic geometry. Using the Rosgen system of channel classification, the two upstream study reaches classified as B4c/1 stream types; which were moderately entrenched, riffle dominated channels, with infrequently spaced pools. The downstream seven study reaches classified as C4/1 stream types; which were slightly entrenched, meandering, gravel-dominated, riffle/ pool channels with well developed flood plains. Analyses of stream reach types suggest that the downstream reaches of the Middle Fork

  12. A Model For Syntectonic Fibrous Vein Growth Inferred From Microtextures. Ouachitas Orogen, Arkansas

    Science.gov (United States)

    Cervantes, P.; Wiltschko, D. V.

    2005-12-01

    Veins from the Lower Ordovician Mazarn Formation in the Arkansas' Ouachitas show two processes of vein growth, 1) continuous localized fracturing and filling and 2) recrystallization, tied to the deformation history of the area under study. Evidence for continuous localized fracturing includes the presence of veinlets near or at the vein-host interface. Veinlets are long and narrow features parallel to the main body of the vein, filled mainly with quartz, between 5 and 25 μm wide. Veinlets cut and displaced host grains. Displacement of host grains is evident because the material precipitated on veinlets show different luminescence than host grains. Remnants of these veinlets are observed in completely recrystallized fibers. Evidence for recrystallization includes, 1) wide fibers (more than 100 μm) with fluid inclusion trails parallel to fiber length that span the entire fiber length, 2) luminescence in the central part of the vein is different than luminescence in the external part of the vein (close to host-vein interface), 3) remnants of original luminescence in the central part of the vein suggesting that recrystallization is a later process. We hypothesize that in this region folding followed by flattening gave rise to boudinage of resistant layers accompanying abundant pressure solution providing the vein forming material. Precipitation of material took place along necks of boudins, which fractured repeatedly allowing veins to grow in width and length. Late thrust faults led to open system conditions which gave rise to new vein growth and recrystallization. Recrystallization altered the shape of previously formed veins.

  13. Progress in research of enterovirus 71 vaccines%EV71疫苗研制新进展

    Institute of Scientific and Technical Information of China (English)

    赵蕾; 陈敬贤; 王明丽

    2013-01-01

    肠道病毒71型(enterovirus 71,EV71)是引起手足口病和神经系统并发症的重要病原体.近几年,世界多个地区发生了由EV72感染引起的手足口病.由于目前尚无有效的抗病毒药物对患者进行治疗,因而疫苗的研制成为预防和控制EV71传播的关键.此文主要就近年EV71疫苗的研制进展做一综述.%Enterovirus 71 (EV71) is known to be a causative agent of hand,foot and mouth disease (HFMD) and severe neurological complications.Many outbreaks caused by EV71 have occurred in different regions of the world.Due to lacking an effective antiviral agent,development of effective vaccines has become the top priority in terms of control strategies.This article reviews the recent advancement in the design of various kinds of vaccines against EV71.

  14. 49 CFR 230.71 - Orifice testing of compressors.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Orifice testing of compressors. 230.71 Section 230... Locomotives and Tenders Brake and Signal Equipment § 230.71 Orifice testing of compressors. (a) Frequency of testing. The compressor or compressors shall be tested for capacity by orifice test as often as...

  15. 21 CFR 1309.71 - General security requirements.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false General security requirements. 1309.71 Section 1309.71 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE REGISTRATION OF... Section, Drug Enforcement Administration. See the Table of DEA Mailing Addresses in § 1321.01 of...

  16. 21 CFR 1301.71 - Security requirements generally.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Security requirements generally. 1301.71 Section 1301.71 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE REGISTRATION OF... the Regulatory Section, Drug Enforcement Administration. See the Table of DEA Mailing Addresses...

  17. 49 CFR 1018.71 - Referral of a compromise offer.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 8 2010-10-01 2010-10-01 false Referral of a compromise offer. 1018.71 Section... § 1018.71 Referral of a compromise offer. The Board may refer a debtor's firm written offer of compromise which is substantial in amount to GAO or to DOJ if the Board is uncertain whether the offer should...

  18. 41 CFR 105-71.141 - Financial reporting.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Financial reporting. 105... GOVERNMENTS 71.14-Post-Award Requirements/Reports, Records, Retention, and Enforcement § 105-71.141 Financial reporting. (a) General. (1) Except as provided in paragraphs (a) (2) and (5) of this section, grantees...

  19. 48 CFR 1452.237-71 - Utilization of Woody Biomass.

    Science.gov (United States)

    2010-10-01

    ... Biomass. 1452.237-71 Section 1452.237-71 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... Utilization of Woody Biomass. As prescribed in § 1437.7202, insert the following clause: Utilization of Woody Biomass (MAY 2005) (a) The contractor may remove and utilize woody biomass, if: (1) Project work...

  20. Therapeutic and prevention strategies against human enterovirus 71 infection.

    Science.gov (United States)

    Kok, Chee Choy

    2015-05-12

    Human enterovirus 71 (HEV71) is the cause of hand, foot and mouth disease and associated neurological complications in children under five years of age. There has been an increase in HEV71 epidemic activity throughout the Asia-Pacific region in the past decade, and it is predicted to replace poliovirus as the extant neurotropic enterovirus of highest global public health significance. To date there is no effective antiviral treatment and no vaccine is available to prevent HEV71 infection. The increase in prevalence, virulence and geographic spread of HEV71 infection over the past decade provides increasing incentive for the development of new therapeutic and prevention strategies against this emerging viral infection. The current review focuses on the potential, advantages and disadvantages of these strategies. Since the explosion of outbreaks leading to large epidemics in China, research in natural therapeutic products has identified several groups of compounds with anti-HEV71 activities. Concurrently, the search for effective synthetic antivirals has produced promising results. Other therapeutic strategies including immunotherapy and the use of oligonucleotides have also been explored. A sound prevention strategy is crucial in order to control the spread of HEV71. To this end the ultimate goal is the rapid development, regulatory approval and widespread implementation of a safe and effective vaccine. The various forms of HEV71 vaccine designs are highlighted in this review. Given the rapid progress of research in this area, eradication of the virus is likely to be achieved. PMID:25964873

  1. 32 CFR 644.71 - Final Title Assembly.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Final Title Assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... Title Assembly. (a) Disposition of final title assemblies. The final title opinion and related...

  2. 29 CFR 570.71 - Occupations involved in agriculture.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Occupations involved in agriculture. 570.71 Section 570.71... CHILD LABOR REGULATIONS, ORDERS AND STATEMENTS OF INTERPRETATION 1-Occupations in Agriculture... agriculture. (a) Findings and declarations of fact as to specific occupations. The following occupations...

  3. 42 CFR 71.54 - Etiological agents, hosts, and vectors.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Etiological agents, hosts, and vectors. 71.54..., INSPECTION, LICENSING FOREIGN QUARANTINE Importations § 71.54 Etiological agents, hosts, and vectors. (a) A... any arthropod or other animal host or vector of human disease, or any exotic living arthropod or...

  4. 14 CFR 23.71 - Glide: Single-engine airplanes.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Glide: Single-engine airplanes. 23.71... AIRWORTHINESS STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Performance § 23.71 Glide: Single-engine airplanes. The maximum horizontal distance traveled in still air, in nautical...

  5. 42 CFR 71.44 - Disinsection of aircraft.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Disinsection of aircraft. 71.44 Section 71.44... Disinsection of aircraft. (a) The Director may require disinsection of an aircraft if it has left a foreign area that is infected with insect-borne communicable disease and the aircraft is suspected of...

  6. 48 CFR 352.239-71 - Standard for encryption language.

    Science.gov (United States)

    2010-10-01

    ... language. 352.239-71 Section 352.239-71 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES... Standard for encryption language. As prescribed in 339.101(d)(2), the Contracting Officer shall insert the following clause: Standard for Encryption Language (January 2010) (a) The Contractor shall use...

  7. 27 CFR 4.71 - Standard wine containers.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Standard wine containers. 4.71 Section 4.71 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS LABELING AND ADVERTISING OF WINE Standards of Fill for Wine §...

  8. 48 CFR 1652.216-71 - Accounting and Allowable Cost.

    Science.gov (United States)

    2010-10-01

    ... of FEHBP Clauses 1652.216-71 Accounting and Allowable Cost. As prescribed in section 1616.7002, the...). Accounting and Allowable Cost (FEHBAR 1652.216-71) (JAN 2003) (a) Annual Accounting Statements. (1) The... addition, the Carrier must: (i) on request, document and make available accounting support for the cost...

  9. Therapeutic and prevention strategies against human enterovirus 71 infection

    Science.gov (United States)

    Kok, Chee Choy

    2015-01-01

    Human enterovirus 71 (HEV71) is the cause of hand, foot and mouth disease and associated neurological complications in children under five years of age. There has been an increase in HEV71 epidemic activity throughout the Asia-Pacific region in the past decade, and it is predicted to replace poliovirus as the extant neurotropic enterovirus of highest global public health significance. To date there is no effective antiviral treatment and no vaccine is available to prevent HEV71 infection. The increase in prevalence, virulence and geographic spread of HEV71 infection over the past decade provides increasing incentive for the development of new therapeutic and prevention strategies against this emerging viral infection. The current review focuses on the potential, advantages and disadvantages of these strategies. Since the explosion of outbreaks leading to large epidemics in China, research in natural therapeutic products has identified several groups of compounds with anti-HEV71 activities. Concurrently, the search for effective synthetic antivirals has produced promising results. Other therapeutic strategies including immunotherapy and the use of oligonucleotides have also been explored. A sound prevention strategy is crucial in order to control the spread of HEV71. To this end the ultimate goal is the rapid development, regulatory approval and widespread implementation of a safe and effective vaccine. The various forms of HEV71 vaccine designs are highlighted in this review. Given the rapid progress of research in this area, eradication of the virus is likely to be achieved. PMID:25964873

  10. 50 CFR 27.71 - Motion or sound pictures.

    Science.gov (United States)

    2010-10-01

    ... provisions of 43 CFR part 5. ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Motion or sound pictures. 27.71 Section 27... Equipment § 27.71 Motion or sound pictures. The taking or filming of any motion or sound pictures on...

  11. Anti-Enterovirus 71 Agents of Natural Products.

    Science.gov (United States)

    Wang, Liyan; Wang, Junfeng; Wang, Lishu; Ma, Shurong; Liu, Yonghong

    2015-01-01

    This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005-2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded. PMID:26370955

  12. Anti-Enterovirus 71 Agents of Natural Products

    Directory of Open Access Journals (Sweden)

    Liyan Wang

    2015-09-01

    Full Text Available This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005–2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded.

  13. The complete genome of Enterovirus 71 China strain

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Five overlapping clones covering the full genome of Enterovirus71 China strain SHZH98 were obtained and then the sequences were determined by the chain termination method. It showed that the full length of EV71 SHZH98 genome (not including Poly A tail) is 7408 bp. There are some diversities on the lengths and sequences of 5′ UTR and 3′ UTR between SHZH98 and the other EV71 strains. In P1 capsid region, which is closely associated with viral immunogenicity, EV71 strain SHZH98 shares the highest homology with Taiwan strains; but in P2 and P3 non-structural gene regions there are higher identities with Coxsakievirus A16 and EV71 strains MS, BrCr than with Taiwan strains. Phylogenetic tree constructed by structural gene region indicates that China strain SHZH98 has a closer relationship with Taiwan strains, however, in the non-coding region it has a closer relationship with Coxsakievirus A16, EV71 strains MS and BrCr. EV71 China strain was analyzed at the molecular level. The results will contribute to the basic study on enteroviruses and the EV71 prevention in China.

  14. 14 CFR 93.71 - General operating procedures.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false General operating procedures. 93.71 Section 93.71 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED..., aircraft operating on an ATC-approved IFR flight plan, aircraft operating the Scenic Falls Route...

  15. Animal models of enterovirus 71 infection: applications and limitations.

    Science.gov (United States)

    Wang, Ya-Fang; Yu, Chun-Keung

    2014-01-01

    Human enterovirus 71 (EV71) has emerged as a neuroinvasive virus that is responsible for several outbreaks in the Asia-Pacific region over the past 15 years. Appropriate animal models are needed to understand EV71 neuropathogenesis better and to facilitate the development of effective vaccines and drugs. Non-human primate models have been used to characterize and evaluate the neurovirulence of EV71 after the early outbreaks in late 1990s. However, these models were not suitable for assessing the neurovirulence level of the virus and were associated with ethical and economic difficulties in terms of broad application. Several strategies have been applied to develop mouse models of EV71 infection, including strategies that employ virus adaption and immunodeficient hosts. Although these mouse models do not closely mimic human disease, they have been applied to determine the pathogenesis of and treatment and prevention of the disease. EV71 receptor-transgenic mouse models have recently been developed and have significantly advanced our understanding of the biological features of the virus and the host-parasite interactions. Overall, each of these models has advantages and disadvantages, and these models are differentially suited for studies of EV71 pathogenesis and/or the pre-clinical testing of antiviral drugs and vaccines. In this paper, we review the characteristics, applications and limitation of these EV71 animal models, including non-human primate and mouse models. PMID:24742252

  16. 46 CFR 64.71 - Marking of pressure relief devices.

    Science.gov (United States)

    2010-10-01

    ... AND CARGO HANDLING SYSTEMS Pressure Relief Devices and Vacuum Relief Devices for MPTs § 64.71 Marking of pressure relief devices. A pressure relief device must be plainly and permanently marked with the... 46 Shipping 2 2010-10-01 2010-10-01 false Marking of pressure relief devices. 64.71 Section...

  17. 44 CFR 71.3 - Denial of flood insurance.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Denial of flood insurance. 71.3 Section 71.3 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program...

  18. 22 CFR 71.7 - Reports on catastrophes abroad.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Reports on catastrophes abroad. 71.7 Section 71... catastrophes abroad. Whenever a great catastrophe occurs abroad, either on land or on sea, the officer within whose district the catastrophe takes place or into whose district the survivors are brought shall...

  19. 48 CFR 552.203-71 - Restriction on Advertising.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Restriction on Advertising. 552.203-71 Section 552.203-71 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses...

  20. National Uranium Resource Evaluation Program. Data report: Arkansas, Louisiana, Mississippi, Missouri, Oklahoma, and Texas. Hydrogeochemical and stream sediment reconnaissance

    International Nuclear Information System (INIS)

    This report presents the results of ground water, stream water, and stream sediment reconnaissance in Arkansas, Louisiana, Mississippi, Missouri, Oklahoma, and Texas. The following samples were collected: Arkansas-3292 stream sediments, 5121 ground waters, 1711 stream waters; Louisiana-1017 stream sediments, 0 ground waters, 0 stream waters; Misissippi-0 stream sediments, 814 ground waters, 0 stream waters; Missouri-2162 stream sediments, 3423 ground waters 1340 stream waters; Oklahoma-2493 stream sediments, 2751 ground waters, 375 stream waters; and Texas-279 stream sediments, 0 ground waters, 0 stream waters. Neutron activation analyses are given for U, Br, Cl, F, Mn, Na, Al, V, and Dy in ground water and stream water, and for U, Th, Hf, Ce, Fe, Mn, Na, Sc, Ti, V, Al, Dy, Eu, La, Sm, Yb, and Lu in sediments. The results of mass spectroscopic analysis for He are given for 563 ground water sites in Mississippi. Field measurements and observations are reported for each site. Oak Ridge National Laboratory analyzed sediment samples which were not analyzed by Savannah River Laboratory neutron activation

  1. Stream habitat and water-quality information for sites in the Buffalo River Basin and nearby basins of Arkansas, 2001-2002

    Science.gov (United States)

    Petersen, James C.

    2004-01-01

    The Buffalo River lies in north-central Arkansas and is a tributary of the White River. Stream-habitat and water-quality information are presented for 52 sites in the Buffalo River Basin and adjacent areas of the White River Basin. The information was collected during the summers of 2001 and 2002 to supplement fish community sampling during the same time period.

  2. The Role of State Government in Agriculture. Proceedings of the Conference on the Role of State Government in Agriculture (Morrilton, Arkansas, December 3-4, 1987).

    Science.gov (United States)

    Ospina, Enrique, Ed.; Sims, Cami S., Ed.

    Conference participants examined how other states are preparing their farmers and rural communities to survive in the present "new world order," and considered whether Arkansas, one of only three states without a central agricultural agency, has the tools to forge strategies for the 1990's. "The Role of State Government in Agriculture" by R. O.…

  3. The complete genome of Enterovirus 71 China strain

    Institute of Scientific and Technical Information of China (English)

    YANG; Fan

    2001-01-01

    [1]Jin Qi, Medical Molecular Virology (in Chinese), Beijing: Science Press, 2001.[2]McMinn, P., Stratov, I., Dowse, G., Enterovirus 71 outbreak in Western Australia associated with acute flaccid paralysis, Preliminary report, Commun. Dis. Intell., 1999, 23(7): 199.[3]Ho, M., Chen, E. R., Hsu, K. H. et al., An epidemic of Enterovirus 71 infection in Taiwan, The New England Journal of Medicine, 1999, 341(13): 929.[4]He Yaqing, Yang Fan, Li Chengliang et al., Identification of EV71 isolates from hand, foot and mouth disease cases in ShenZhen, Chinese Journal of Experimental and Clinical Virology (in Chinese), 1999, 1: 112.[5]Landry, M. L., Fonseca, S. N. S., Cohen, S. et al., Fatal enterovirus type 71 infection: rapid detection and diagnostic pitfalls, Pediatr. Infect. Dis. J., 1995, 14: 1095.[6]Brown, B. A., Pallansch, M. A., Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus, Virus Res., 1995, 39: 195.[7]Brown, B. A., Oberste, M. S., Alexander, J. P. Jr., Molecular epidemiology and evolution of Enterovirus 71 strains isolated from 1970 to 1998, J. Virol., 1999, 73(12): 9969.[8]Ishimaru,Y., Nakano,S., Yamaoda,K.et al.,Outbreaks of hand,foot,and mouth disease by Enterovirus 71,Arch.Dis.Child.,1980,55:583.

  4. Enterovirus 71 infection: An experience in Korea, 2009

    Directory of Open Access Journals (Sweden)

    Kyung Hyo Kim

    2010-05-01

    Full Text Available Enterovirus 71 (EV71 has been recognized as a frequent cause of epidemics of hand-foot-and-mouth disease (HFMD associated with severe neurological symptoms. In the spring of 2009, HFMD was epidemic in Korea. Severe cases with complication, including death, have been reported and it has become a public health issue. Most symptomatic EV71 infections commonly result in HFMD or herpangina. These clinical manifestations can be associated with neurologic syndromes frequently. Neurologic syndromes observed in EV71 include meningitis, meningoencephalomyelitis, poliomyelitis-like paralytic disease, Guillain-Barr&eacute; syndrome, transverse myelitis, cerebellar ataxia, opsoclonus-myoclonus syndrome, benign intracranial hypertension, and brainstem encephalitis. Examinations for EV 71 were performed from the stools, respiratory secretion or CSF of the children by realtime PCR. Gene analysis showed that most of them were caused by EV71 subgenotype C4a which was prevalent in China, 2008. Public health measures including personal and environmental hygiene, must to target daycare centers, kindergartens, and schools where highly susceptible children congregate. To prevent the spread of infection, preschools where transmission persists for more than 2 incubation periods, have been recommended for closure, and trigger criteria for voluntary closure was instituted. During closure, operators are to thoroughly clean the centers before they are allowed to reopen. In addition, parents are advised to ensure that their children adopt a high standard of personal hygiene and to keep the infected child at home until full recovery. Because the outbreaks occur in a cyclical pattern, surveillance system to predict next outbreaks and adequate public health measures to control need to be planned for future. Control of EV71 epidemics through surveillance and public health intervention needs to be maintained in Korea. Future research should focus on understanding of EV71

  5. Inhibition of enterovirus 71 entry by transcription factor XBP1

    International Nuclear Information System (INIS)

    Highlights: ► IRE1 was activated but no XBP1 splicing was detected during enterovirus 71 infection. ► XBP1 was subject to translational shutoff by enterovirus 71-induced eIF4G cleavage. ► The uptake of UV-irradiated virus was decreased in XBP1-overexpressing cells. -- Abstract: Inositol-requiring enzyme 1 (IRE1) plays an important role in the endoplasmic reticulum (ER), or unfolded protein, stress response by activating its downstream transcription factor X-box-binding protein 1 (XBP1). We demonstrated previously that enterovirus 71 (EV71) upregulated XBP1 mRNA levels but did not activate spliced XBP1 (XBP1s) mRNA or its downstream target genes, EDEM and chaperones. In this study, we investigated further this regulatory mechanism and found that IRE1 was phosphorylated and activated after EV71 infection, whereas its downstream XBP1s protein level decreased. We also found that XBP1s was not cleaved directly by 2Apro, but that cleavage of eukaryotic translation initiation factor 4G by the EV71 2Apro protein may contribute to the decrease in XBP1s expression. Knockdown of XBP1 increased viral protein expression, and the synthesis of EV71 viral protein and the production of EV71 viral particles were inhibited in XBP1-overexpressing RD cells. When incubated with replication-deficient and UV-irradiated EV71, XBP1-overexpressing RD cells exhibited reduced viral RNA levels, suggesting that the inhibition of XBP1s by viral infection may underlie viral entry, which is required for viral replication. Our findings are the first indication of the ability of XBP1 to inhibit viral entry, possibly via its transcriptional activity in regulating molecules in the endocytic machinery.

  6. Inhibition of enterovirus 71 entry by transcription factor XBP1

    Energy Technology Data Exchange (ETDEWEB)

    Jheng, Jia-Rong; Lin, Chiou-Yan [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China); Horng, Jim-Tong, E-mail: jimtong@mail.cgu.edu.tw [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China); Lau, Kean Seng [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China)

    2012-04-20

    Highlights: Black-Right-Pointing-Pointer IRE1 was activated but no XBP1 splicing was detected during enterovirus 71 infection. Black-Right-Pointing-Pointer XBP1 was subject to translational shutoff by enterovirus 71-induced eIF4G cleavage. Black-Right-Pointing-Pointer The uptake of UV-irradiated virus was decreased in XBP1-overexpressing cells. -- Abstract: Inositol-requiring enzyme 1 (IRE1) plays an important role in the endoplasmic reticulum (ER), or unfolded protein, stress response by activating its downstream transcription factor X-box-binding protein 1 (XBP1). We demonstrated previously that enterovirus 71 (EV71) upregulated XBP1 mRNA levels but did not activate spliced XBP1 (XBP1s) mRNA or its downstream target genes, EDEM and chaperones. In this study, we investigated further this regulatory mechanism and found that IRE1 was phosphorylated and activated after EV71 infection, whereas its downstream XBP1s protein level decreased. We also found that XBP1s was not cleaved directly by 2A{sup pro}, but that cleavage of eukaryotic translation initiation factor 4G by the EV71 2A{sup pro} protein may contribute to the decrease in XBP1s expression. Knockdown of XBP1 increased viral protein expression, and the synthesis of EV71 viral protein and the production of EV71 viral particles were inhibited in XBP1-overexpressing RD cells. When incubated with replication-deficient and UV-irradiated EV71, XBP1-overexpressing RD cells exhibited reduced viral RNA levels, suggesting that the inhibition of XBP1s by viral infection may underlie viral entry, which is required for viral replication. Our findings are the first indication of the ability of XBP1 to inhibit viral entry, possibly via its transcriptional activity in regulating molecules in the endocytic machinery.

  7. Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates

    Directory of Open Access Journals (Sweden)

    Ai-Hsiang Chou

    2012-01-01

    Full Text Available Enterovirus 71 (EV71 and coxsackievirus A16 (CVA16 are major causative agents of hand, foot, and mouth diseases (HFMDs, and EV71 is now recognized as an emerging neurotropic virus in Asia. Effective medications and/or prophylactic vaccines against HFMD are not available. The current results from mouse immunogenicity studies using in-house standardized RD cell virus neutralization assays indicate that (1 VP1 peptide (residues 211–225 formulated with Freund’s adjuvant (CFA/IFA elicited low virus neutralizing antibody response (1/32 titer; (2 recombinant virus-like particles produced from baculovirus formulated with CFA/IFA could elicit good virus neutralization titer (1/160; (3 individual recombinant EV71 antigens (VP1, VP2, and VP3 formulated with CFA/IFA, only VP1 elicited antibody response with 1/128 virus neutralization titer; and (4 the formalin-inactivated EV71 formulated in alum elicited antibodies that cross-neutralized different EV71 genotypes (1/640, but failed to neutralize CVA16. In contrast, rabbits antisera could cross-neutralize strongly against different genotypes of EV71 but weakly against CVA16, with average titers 1/6400 and 1/32, respectively. The VP1 amino acid sequence dissimilarity between CVA16 and EV71 could partially explain why mouse antibodies failed to cross-neutralize CVA16. Therefore, the best formulation for producing cost-effective HFMD vaccine is a combination of formalin-inactivated EV71 and CAV16 virions.

  8. The Occurrence of Knickpoints in Soluble Strata in the Buffalo River Basin, Arkansas

    Science.gov (United States)

    Thaler, E.; Covington, M. D.; Myre, J. M.; Perne, M.; Holcomb, G.

    2014-12-01

    Prior field and theoretical work has suggested that bedrock channels adjust to lower stream power when encountering highly soluble strata, exhibiting an increase in channel width and/or a decrease in channel slope. However, in apparent contradiction to this expectation, many channels within the Buffalo River Basin, Arkansas, contain knickpoints, in the form of waterfalls and slot canyons, that are developed at the contact between the Mississippian Batesville Sandstone and the underlying Boone Limestone. To improve understanding of bedrock channel response to contrasts in rock solubility, longitudinal surveys were conducted in three channels that cross the Boone Limestone. Additionally, channel widths and a profile were obtained for the main stem of the Buffalo River using aerial photography and a digital elevation model. Schmidt scores for the Boone and Batesville suggest that the two strata have similar compressive strengths, which is a measure of relative resistance to mechanical erosion. Two of the four studied reaches show significant knickpoint development, and in both cases the basin area above the knickpoint is less than 3 km2. One possible explanation is that these knickpoints have been arrested at a critical threshold basin area. However, at least four other such knickpoints are known from the area, and in all cases the knickpoint is highly correlated to the contact rather than a specific basin area, suggesting that the properties of the strata are an important factor. We identify three potential mechanisms that may often act in concert to develop knickpoints at contacts with underlying soluble rocks. (1) If chemical erosion in the soluble reach outpaces uplift, and knickpoint retreat through the overlying layer is sufficiently slow, then a knickpoint will develop. (2) Karstification can divert geomorphic work to the subsurface, resulting in a steep surface channel and possible stalling of upstream knickpoint migration within the soluble strata. (3) The

  9. 112 The Development of Enterovirus 71 Vaccine

    OpenAIRE

    Xiuling, Li; Zhongyang, Zhang; Xiaoxiao, Wang; Chunsheng, Hao; Yi, Li; Chong, Zhang; Chen, Zhang; Ming, Chen; haijing, Ning; Yu, Liu; Lei, Chen; Shuhua, Ma; Yongjuan, Yang; Dongmei, Song; Xinliang, Shen

    2014-01-01

    Enterovirus 71 (EV71) is a major cause of epidemic outbreaks of hand-foot-and-mouth disease (HFMD) worldwide. The virus belongs to the family Picornaviridae, genus Enterovirus. 3 genotypes—A, B, and C and more than 10 sub-genotypes have been identified. After the initial identification of this virus in 1969, some large outbreaks of HFMD have been reported worldwide. EV71-induced HFMD is usually characterized by the formation of maculopapular or vesicular lesions on the skin and oral mucosa, e...

  10. Fatal Case of Enterovirus 71 Infection, France, 2007

    OpenAIRE

    Vallet, Sophie; Legrand-Quillien, Marie-Christine; Dailland, Thomas; Podeur, Gaëtan; Gouriou, Stéphanie; Schuffenecker, Isabelle; Payan, Christopher; Marcorelles, Pascale

    2009-01-01

    A fatal case of enterovirus 71 infection with pulmonary edema and rhombencephalitis occurred in Brest, France, in April 2007. The virus was identified as subgenogroup C2. This highly neurotropic enterovirus merits specific surveillance outside the Asia-Pacific region.

  11. Antiviral Effect of Matrine against Human Enterovirus 71

    Directory of Open Access Journals (Sweden)

    Jiangning Liu

    2012-08-01

    Full Text Available Human enterovirus 71, a member of the Picornaviridae family, is one of the major causative agent of hand, foot and mouth disease in children less than six years old. This illness has caused mortalities in large-scale outbreaks in the Asia-Pacific region in recent years. No vaccine or antiviral therapy is available. In this study, antiviral effect of matrine against enterovirus 71 were evaluated in vitro and in vivo. Matrine could suppress the viral RNA copy number on rhabdomyosarcoma cells. Moreover, matrine treatment of mice challenged with a lethal dose of enterovirus 71 reduced the mortality and relieved clinical symptoms. The results showed that matrine may represent a potential therapeutic agent for enterovirus 71 infection.

  12. Status of research and development of vaccines for enterovirus 71.

    Science.gov (United States)

    Reed, Zarifah; Cardosa, Mary Jane

    2016-06-01

    Although outbreaks of Hand, Foot, and Mouth Disease (HFMD) in young children have long been recognized worldwide, the occurrence of rare and life-threatening neurological, respiratory, and cardiac complications has propelled this common condition into the spotlight as a major public health problem in the affected countries. Various enteroviruses cause HFMD, but the severe complications have been mostly associated with enterovirus 71 (EV71). Medical treatment is supportive and measures to interrupt transmission have been challenging to implement. Preventive vaccines could have an important clinical impact, especially among children younger than 3 years old who are most susceptible to the neurological complications. Several groups in the highly affected Asia-Pacific region are working towards vaccines against EV71 and some candidates have progressed to late-stage clinical trials with two vaccines recently reported to have been approved by the regulatory authorities in China. This report summarizes current issues and progress in the development of vaccines against EV71. PMID:26973065

  13. Enterovirus 71 Brainstem Encephalitis and Cognitive and Motor Deficits

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2006-12-01

    Full Text Available Follow-up studies were conducted in 63 previously healthy children with enterovirus 71 brainstem encephalitis (49 stage II, 7 stage Ilia, and 7 stage Illb at National Cheng Kung University Hospital, Tainan, Taiwan.

  14. 48 CFR 915.404-4-71-1 - General.

    Science.gov (United States)

    2010-10-01

    ... CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to...

  15. Pathological examinations of an enterovirus 71 infection: an autopsy case

    OpenAIRE

    Gao, Lulu; Lin, Peixin; Liu, Shuguang; Lei, Bin; Chen, Qing; Yu, Shouyi; SHEN Hong

    2014-01-01

    We report an 8-month-old female infant with the fatal enterovirus 71 infection here. Clinically, she developed respiratory failure and severe pulmonary edema rapidly. Histologically, the lung specimen showed diffuse, severe pulmonary congestion and edema with focal intra-alveolar hemorrhage and typical features of acute encephalitis were easily identified under light microscope. Immunohistochemically, enterovirus 71 antigen was positive in the cerebella and brainstem. We measured the viral lo...

  16. Ethanol and water adsorption in methanol-derived ZIF-71

    KAUST Repository

    Lively, Ryan P.

    2011-01-01

    A room temperature method for synthesizing zeolitic imidizolate framework 71 (ZIF-71) is described. The methanol-based synthesis results in >95% yields (based on Zn) and produces crystals with 70% greater surface area than reported earlier. Ethanol uptake into the ZIF compares favorably with a recent modeling-based study. Water uptake was significantly higher than model predictions. © The Royal Society of Chemistry 2011.

  17. Enterovirus 71 viral capsid protein linear epitopes: Identification and characterization

    Directory of Open Access Journals (Sweden)

    Gao Fan

    2012-01-01

    Full Text Available Abstract Background To characterize the human humoral immune response against enterovirus 71 (EV71 infection and map human epitopes on the viral capsid proteins. Methods A series of 256 peptides spanning the capsid proteins (VP1, VP2, VP3 of BJ08 strain (genomic C4 were synthesized. An indirect enzyme-linked immunosorbent assay (ELISA was carried out to detect anti-EV71 IgM and IgG in sera of infected children in acute or recovery phase. The partially overlapped peptides contained 12 amino acids and were coated in the plate as antigen (0.1 μg/μl. Sera from rabbits immunized with inactivated BJ08 virus were also used to screen the peptide panel. Results A total of 10 human anti-EV71 IgM epitopes (vp1-14 in VP1; vp2-6, 21, 40 and 50 in VP2 and vp3-10, 12, 15, 24 and 75 in VP3 were identified in acute phase sera. In contrast, only one anti-EV71 IgG epitope in VP1 (vp1-15 was identified in sera of recovery stage. Four rabbit anti-EV71 IgG epitopes (vp1-14, 31, 54 and 71 were identified and mapped to VP1. Conclusion These data suggested that human IgM epitopes were mainly mapped to VP2 and VP3 with multi-epitope responses occurred at acute infection, while the only IgG epitope located on protein VP1 was activated in recovery phase sera. The dynamic changes of humoral immune response at different stages of infection may have public health significance in evaluation of EV71 vaccine immunogenicity and the clinical application of diagnostic reagents.

  18. Clinical manifestations of CNS infections caused by enterovirus type 71

    OpenAIRE

    Cheol Soon Choi; Yun Jung Choi; Ui Yoon Choi; Ji Whan Han; Dae Chul Jeong; Hyun Hee Kim; Jong Hyun Kim; Jin Han Kang

    2011-01-01

    Purpose: Enterovirus 71, one of the enteroviruses that are responsible for both hand-foot-and-mouth disease and herpangina, can cause neural injury. During periods of endemic spread of hand-foot-andmouth disease caused by enterovirus 71, CNS infections are also frequently diagnosed and may lead to increased complications from neural injury, as well as death. We present the results of our epidemiologic research on the clinical manifestations of children with CNS infections caused by enteroviru...

  19. Atomic Mass and Nuclear Binding Energy for Sr-71 (Strontium)

    Science.gov (United States)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume A `Nuclei with Z = 1 - 54' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms'. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Sr-71 (Strontium, atomic number Z = 38, mass number A = 71).

  20. Genome analysis of enterovirus 71 strains differing in mouse pathogenicity.

    Science.gov (United States)

    Li, Peng; Yue, Yingying; Song, Nannan; Li, Bingqing; Meng, Hong; Yang, Guiwen; Li, Zhihui; An, Liguo; Qin, Lizeng

    2016-04-01

    Enterovirus 71 (EV71) is a major causative agent of hand, foot, and mouth disease (HFMD) and is occasionally associated with severe neurological diseases. The investigation of virulence determinants of EV71 is rudimentary. Therefore, it is important to understand the relationship between EV71 virulence and genomic information. In this study, a series of analyses about full-length genomic sequence were performed on six EV71 strains isolated from HFMD patients with either severe or mild clinical symptoms. A one-day-old BALB/c mouse model was used to study the infection characteristics. Results showed all six strains were of the subgenogroup C4a. Viral full-length genomic sequence analysis showed that a total of 40 nucleotide differences between strains of highly and low virulence were revealed. Among all mutations, three nucleotide mutations were found in the untranslated region. A mutation, nt115, at internal ribozyme entry site (IRES) caused RNA secondary structural change. The other 37 mutations were all located in the open reading frame resulting in 8 amino acid mutations. Importantly, we discovered that a mutation of amino acid (Asn1617 → Asp1617) in the 3C proteinase (3C(pro)) of highly and low pathogenic strains could lead to conformational change at the active center, suggesting that this site may be a virulence determinant of EV71. PMID:26781949

  1. Innate Immunity and Immune Evasion by Enterovirus 71

    Directory of Open Access Journals (Sweden)

    Prabuddha S. Pathinayake

    2015-12-01

    Full Text Available Enterovirus 71 (EV71 is a major infectious disease affecting millions of people worldwide and it is the main etiological agent for outbreaks of hand foot and mouth disease (HFMD. Infection is often associated with severe gastroenterological, pulmonary, and neurological diseases that are most prevalent in children. Currently, no effective vaccine or antiviral drugs exist against EV71 infection. A lack of knowledge on the molecular mechanisms of EV71 infection in the host and the virus-host interactions is a major constraint to developing specific antiviral strategies against this infection. Previous studies have identified and characterized the function of several viral proteins produced by EV71 that interact with the host innate immune proteins, including type I interferon signaling and microRNAs. These interactions eventually promote efficient viral replication and increased susceptibility to the disease. In this review we discuss the functions of EV71 viral proteins in the modulation of host innate immune responses to facilitate viral replication.

  2. Innate Immunity and Immune Evasion by Enterovirus 71.

    Science.gov (United States)

    Pathinayake, Prabuddha S; Hsu, Alan C-Y; Wark, Peter A B

    2015-12-01

    Enterovirus 71 (EV71) is a major infectious disease affecting millions of people worldwide and it is the main etiological agent for outbreaks of hand foot and mouth disease (HFMD). Infection is often associated with severe gastroenterological, pulmonary, and neurological diseases that are most prevalent in children. Currently, no effective vaccine or antiviral drugs exist against EV71 infection. A lack of knowledge on the molecular mechanisms of EV71 infection in the host and the virus-host interactions is a major constraint to developing specific antiviral strategies against this infection. Previous studies have identified and characterized the function of several viral proteins produced by EV71 that interact with the host innate immune proteins, including type I interferon signaling and microRNAs. These interactions eventually promote efficient viral replication and increased susceptibility to the disease. In this review we discuss the functions of EV71 viral proteins in the modulation of host innate immune responses to facilitate viral replication. PMID:26694447

  3. Cytokine Immunopathogenesis of Enterovirus 71 Brain Stem Encephalitis

    Directory of Open Access Journals (Sweden)

    Shih-Min Wang

    2012-01-01

    Full Text Available Enterovirus 71 (EV71 is one of the most important causes of herpangina and hand, foot, and mouth disease. It can also cause severe complications of the central nervous system (CNS. Brain stem encephalitis with pulmonary edema is the severe complication that can lead to death. EV71 replicates in leukocytes, endothelial cells, and dendritic cells resulting in the production of immune and inflammatory mediators that shape innate and acquired immune responses and the complications of disease. Cytokines, as a part of innate immunity, favor the development of antiviral and Th1 immune responses. Cytokines and chemokines play an important role in the pathogenesis EV71 brain stem encephalitis. Both the CNS and the systemic inflammatory responses to infection play important, but distinctly different, roles in the pathogenesis of EV71 pulmonary edema. Administration of intravenous immunoglobulin and milrinone, a phosphodiesterase inhibitor, has been shown to modulate inflammation, to reduce sympathetic overactivity, and to improve survival in patients with EV71 autonomic nervous system dysregulation and pulmonary edema.

  4. Geodatabase of the datasets used to represent the six subunits of the Texas Coastal Uplands and Mississippi Embayment aquifer system, Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, Missouri, Tennessee, and Texas

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This geodatabase includes spatial datasets that represent the Texas Coastal Uplands and Mississippi Embayment aquifer system in the States of Alabama, Arkansas,...

  5. Viral kinetics of Enterovirus 71 in human habdomyosarcoma cells

    Institute of Scientific and Technical Information of China (English)

    Jing Lu; Ya-Qing He; Li-Na Yi; Hong Zan; Hsiang-Fu Kung; Ming-Liang He

    2011-01-01

    AIM: To characterise the viral kinetics of enterovirus 71 (EV71). METHODS: In this study, human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI). After infection, the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD camera at different time points post viral infection (0, 6, 12, 24 h post infection). Cell growth and viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in both EV71 infected and mock infected cells at each time point. EV71 replication kinetics in RD cells was determined by measuring the total intracellular viral RNA with real-time reverse-transcription polymerase chain reaction (qRT-PCR). Also, the intracellular and extracellular virion RNA was isolated and quantified at different time points to analyze the viral package and secretion. The expression of viral protein was determined by analyze the levels of viral structure protein VP1 with Western blotting. RESULTS: EV71 infection induced a significant CPE as early as 6 h post infection (p.i.) in both RD cells infected with high ratio of virus (MOI 10) and low ratio of virus (MOI 1). In EV71 infected cells, the cell growth was inhibited and the number of viable cells was rapidly decreased in the later phase of infection. EV71 virions were uncoated immediately after entry. The intracellular viral RNA began to increase at as early as 3 h p.i. and the exponential increase was found between 3 h to 6 h p.i. in both infected groups. For viral structure protein synthesis, results from western-blot showed that intracellular viral protein VP1 could not be detected until 6 h p.i. in the cells infected at either MOI 1 or MOI 10; and reached the peak at 9 h p.i. in the cells infected with EV71 at both MOI 1 and MOI 10. Simultaneously, the viral package and secretion were also actively processed as the virus underwent rapid replication. The viral package kinetics

  6. Adaptation of enterovirus 71 to adult interferon deficient mice.

    Directory of Open Access Journals (Sweden)

    Elizabeth A Caine

    Full Text Available Non-polio enteroviruses, including enterovirus 71 (EV71, have caused severe and fatal cases of hand, foot and mouth disease (HFMD in the Asia-Pacific region. The development of a vaccine or antiviral against these pathogens has been hampered by the lack of a reliable small animal model. In this study, a mouse adapted EV71 strain was produced by conducting serial passages through A129 (α/β interferon (IFN receptor deficient and AG129 (α/β, γ IFN receptor deficient mice. A B2 sub genotype of EV71 was inoculated intraperitoneally (i.p. into neonatal AG129 mice and brain-harvested virus was subsequently passaged through 12 and 15 day-old A129 mice. When tested in 10 week-old AG129 mice, this adapted strain produced 100% lethality with clinical signs including limb paralysis, eye irritation, loss of balance, and death. This virus caused only 17% mortality in same age A129 mice, confirming that in the absence of a functional IFN response, adult AG129 mice are susceptible to infection by adapted EV71 isolates. Subsequent studies in adult AG129 and young A129 mice with the adapted EV71 virus examined the efficacy of an inactivated EV71 candidate vaccine and determined the role of humoral immunity in protection. Passive transfer of rabbit immune sera raised against the EV71 vaccine provided protection in a dose dependent manner in 15 day-old A129 mice. Intramuscular injections (i.m. in five week-old AG129 mice with the alum adjuvanted vaccine also provided protection against the mouse adapted homologous strain. No clinical signs of disease or mortality were observed in vaccinated animals, which received a prime-and-boost, whereas 71% of control animals were euthanized after exhibiting systemic clinical signs (P<0.05. The development of this animal model will facilitate studies on EV71 pathogenesis, antiviral testing, the evaluation of immunogenicity and efficacy of vaccine candidates, and has the potential to establish correlates of protection

  7. Hydrogeochemical and stream-sediment reconnaissance, orientation study, Ouachita Mountain area, Arkansas. National Uranium Resource Evaluation Program

    Energy Technology Data Exchange (ETDEWEB)

    Steele, K. F.

    1982-08-01

    A hydrogeochemical ground water orientation study was conducted in the multi-mineralized area of the Ouachita Mountains, Arkansas in order to evaluate the usefulness of ground water as a sampling medium for uranium exploration in similar areas. Ninety-three springs and nine wells were sampled in Clark, Garland, Hot Springs, Howard, Montgomery, Pike, Polk, and Sevier Counties. Manganese, barite, celestite, cinnabar, stibnite, copper, lead, and zinc are present. The following parameters were determined: pH, conductivity, alkalinity, U, Br, Cl, F, He, Mn, Na, V, Al, Dy, NO/sub 3/, NH/sub 3/, SO/sub 4/, and PO/sub 4/. The minerals appear to significantly affect the chemistry of the ground water. This report is issued in draft form, without detailed technical and copy editing. This was done to make the report available to the public before the end of the National Uranium Resource Evaluation.

  8. Metals, Parasites, and Environmental Conditions Affecting Breeding Populations of Spotted Salamanders (Ambystoma maculatum) in Northern Arkansas, USA.

    Science.gov (United States)

    DeMali, Heather M; Trauth, Stanley E; Bouldin, Jennifer L

    2016-06-01

    The spotted salamander (Ambystoma maculatum) is indigenous to northern Arkansas, and several breeding sites are known to exist in the region. Spotted salamanders (n = 17) were collected and examined for parasites and only three females harbored nematodes (Physaloptera spp.). Chronic aquatic bioassays were conducted using water collected from eight breeding ponds during different hydroperiod events. No lethal or sublethal effects were measured in Ceriodaphnia dubia; however, decreased growth and survival were seen in Pimephales promelas. Aqueous, sediment, and salamander hepatic samples were analyzed for As, Cd, Cu, Pb, and Ni. Metal analysis revealed possible increased metal exposure following precipitation, with greatest metal concentrations measured in sediment samples. Hepatic metal concentrations were similar in parasitized and non-parasitized individuals, and greatest Pb concentrations were measured following normal precipitation events. Determining environmental stressors of amphibians, especially during their breeding and subsequent larval life stage, is imperative to improve species conservation. PMID:26886425

  9. Hydrogeochemical and stream-sediment reconnaissance, orientation study, Ouachita Mountain area, Arkansas. National Uranium Resource Evaluation Program

    International Nuclear Information System (INIS)

    A hydrogeochemical ground water orientation study was conducted in the multi-mineralized area of the Ouachita Mountains, Arkansas in order to evaluate the usefulness of ground water as a sampling medium for uranium exploration in similar areas. Ninety-three springs and nine wells were sampled in Clark, Garland, Hot Springs, Howard, Montgomery, Pike, Polk, and Sevier Counties. Manganese, barite, celestite, cinnabar, stibnite, copper, lead, and zinc are present. The following parameters were determined: pH, conductivity, alkalinity, U, Br, Cl, F, He, Mn, Na, V, Al, Dy, NO3, NH3, SO4, and PO4. The minerals appear to significantly affect the chemistry of the ground water. This report is issued in draft form, without detailed technical and copy editing. This was done to make the report available to the public before the end of the National Uranium Resource Evaluation

  10. Analysis and inundation mapping of the April-May 2011 flood at selected locations in northern and eastern Arkansas and southern Missouri

    Science.gov (United States)

    Westerman, Drew A.; Merriman, Katherine R.; De Lanois, Jeanne L.; Berenbrock, Charles

    2013-01-01

    Precipitation that fell from April 19 through May 3, 2011, resulted in widespread flooding across northern and eastern Arkansas and southern Missouri. The first storm produced a total of approximately 16 inches of precipitation over an 8-day period, and the following storms produced as much as 12 inches of precipitation over a 2-day period. Moderate to major flooding occurred quickly along many streams within Arkansas and Missouri (including the Black, Cache, Illinois, St. Francis, and White Rivers) at levels that had not been seen since the historic 1927 floods. The 2011 flood claimed an estimated 21 lives in Arkansas and Missouri, and damage caused by the flooding resulted in a Federal Disaster Declaration for 59 Arkansas counties that received Federal or State assistance. To further the goal of documenting and understanding floods, the U.S. Geological Survey, in cooperation with the Federal Emergency Management Agency, the U.S. Army Corps of Engineers–Little Rock and Memphis Districts, and Arkansas Natural Resources Commission, conducted a study to summarize meteorological and hydrological conditions before the flood; computed flood-peak magnitudes for 39 streamgages; estimated annual exceedance probabilities for 37 of those streamgages; determined the joint probabilities for 11 streamgages paired to the Mississippi River at Helena, Arkansas, which refers to the probability that locations on two paired streams simultaneously experience floods of a magnitude greater than or equal to a given annual exceedance probability; collected high-water marks; constructed flood-peak inundation maps showing maximum flood extent and water depths; and summarized flood damages and effects. For the period of record used in this report, peak-of-record stage occurred at 24 of the 39 streamgages, and peak-of-record streamflow occurred at 13 of the 30 streamgages where streamflow was determined. Annual exceedance probabilities were estimated to be less than 0.5 percent at three

  11. Producing infectious enterovirus type 71 in a rapid strategy

    Directory of Open Access Journals (Sweden)

    Qin E-De

    2010-06-01

    Full Text Available Abstract Background Enterovirus 71 (EV71 is an etiologic agent of hand-foot-and-mouth disease (HFMD, and recent HFMD epidemics worldwide have been associated with a severe form of brainstem encephalitis associated with pulmonary edema and high case-fatality rates. EV71 contains a positive-sense single-stranded genome RNA of approximately 7400 bp in length which encodes a polyprotein with a single open reading frame (ORF, which is flanked by untranslated regions at both the 5' and 3' ends. Results A long distance RT-PCR assay was developed to amplify the full length genome cDNA of EV71 by using specific primes carrying a SP6 promoter. Then the in vitro synthesized RNA transcripts from the RT-PCR amplicons were then transfected into RD cells to produce the rescued virus. The rescued virus was further characterized by RT-PCR and indirect fluorescent-antibody (IFA assay in comparison with the wild type virus. The rescued viruses were infectious on RD cells and neurovirulent when intracerebrally injected into suckling mice. Conclusions Thus, we established a rapid method to produce the infectious full length cDNA of EV71 directly from RNA preparations and specific mutations can be easily engineered into the rescued enterovirus genome by this method.

  12. Similar protective immunity induced by an inactivated enterovirus 71 (EV71) vaccine in neonatal rhesus macaques and children.

    Science.gov (United States)

    Zhang, Ying; Wang, Lichun; Liao, Yun; Liu, Longding; Ma, Kaili; Yang, Erxia; Wang, Jingjing; Che, Yanchun; Jiang, Li; Pu, Jing; Guo, Lei; Feng, Min; Liang, Yan; Cui, Wei; Yang, Huai; Li, Qihan

    2015-11-17

    During the development of enterovirus 71 (EV71) inactivated vaccine for preventing human hand, foot and mouth diseases (HFMD) by EV71 infection, an effective animal model is presumed to be significant and necessary. Our previous study demonstrated that the vesicles in oral regions and limbs potentially associated with viremia, which are the typical manifestations of HFMD, and remarkable pathologic changes were identified in various tissues of neonatal rhesus macaque during EV71 infection. Although an immune response in terms of neutralizing antibody and T cell memory was observed in animals infected by the virus or stimulated by viral antigen, whether such a response could be considered as an indicator to justify the immune response in individuals vaccinated or infected in a pandemic needs to be investigated. Here, a comparative analysis of the neutralizing antibody response and IFN-γ-specific T cell response in vaccinated neonatal rhesus macaques and a human clinical trial with an EV71 inactivated vaccine was performed, and the results showed the identical tendency and increased level of neutralizing antibody and the IFN-γ-specific T cell response stimulated by the EV71 antigen peptide. Importantly, the clinical protective efficacy against virus infection by the elicited immune response in the immunized population compared with the placebo control and the up-modulated gene profile associated with immune activation were similar to those in infected macaques. Further safety verification of this vaccine in neonatal rhesus macaques and children confirmed the potential use of the macaque as a reliable model for the evaluation of an EV71 candidate vaccine. PMID:26419198

  13. SR-71黑鸟飞机

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    SR-71“黑鸟”(BlackBird),美国空军高空高速侦察机。飞行高度达到30000米,最大速度达到3.5倍音速,这称之为“双三”。因此SR-71比现有绝大多数战斗机和防空导弹都要飞得高、飞得快,因此出入敌国领空如入无人之境;在以色列上空侦察以色列核设施时,以军F-14战斗机向它发射了AIM-9“响尾蛇”空空导弹,但是导弹飞得却还比SR-71慢。

  14. Clinical manifestations of CNS infections caused by enterovirus type 71

    Directory of Open Access Journals (Sweden)

    Cheol Soon Choi

    2011-01-01

    Full Text Available Purpose: Enterovirus 71, one of the enteroviruses that are responsible for both hand-foot-and-mouth disease and herpangina, can cause neural injury. During periods of endemic spread of hand-foot-andmouth disease caused by enterovirus 71, CNS infections are also frequently diagnosed and may lead to increased complications from neural injury, as well as death. We present the results of our epidemiologic research on the clinical manifestations of children with CNS infections caused by enterovirus 71. Methods: The study group consisted of 42 patients admitted for CNS infection by enterovirus 71 between April 2009 and October 2009 at the Department of Pediatrics of 5 major hospitals affiliated with the Catholic University of Korea. We retrospectively reviewed initial symptoms and laboratory findings on admission, the specimen from which enterovirus 71 was isolated, fever duration, admission period, treatment and progress, and complications. We compared aseptic meningitis patients with encephalitis patients. Results: Of the 42 patients (23 men, 19 women, hand-foot-and-mouth disease was most prevalent (n=39, followed by herpangina (n=3, upon initial clinical diagnosis. Among the 42 patients, 15 (35.7% were classified as severe, while 27 (64.3% were classified as mild. Factors such as age, fever duration, presence of seizure, and use of intravenous immunoglobulin (IVIG were statistically different between the 2 groups. Conclusion: Our results indicate that patients with severe infection caused by enterovirus 71 tended to be less than 3 years old, presented with at least 3 days of fever as well as seizure activity, and received IVIG treatment.

  15. Edward (Ed) T. Schneider in Front of SR-71 Blackbird

    Science.gov (United States)

    1995-01-01

    SR-71 research pilot Ed Schneider is pictured here in front of an SR-71 Blackbird on the ramp at the Dryden Flight Research Center, Edwards, California. Schneider became a NASA research pilot at Dryden in 1983. Data from the SR-71 program will be used to aid designers of future supersonic aircraft and propulsion systems. He retired as a NASA research pilot in September 2000. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or

  16. Antiviral Effect of Matrine against Human Enterovirus 71

    OpenAIRE

    Jiangning Liu; Chuan Qin; Kai Yan; Liangfeng Zhang; Xu Zhang; Jinghui Xiu; Yajun Yang

    2012-01-01

    Human enterovirus 71, a member of the Picornaviridae family, is one of the major causative agent of hand, foot and mouth disease in children less than six years old. This illness has caused mortalities in large-scale outbreaks in the Asia-Pacific region in recent years. No vaccine or antiviral therapy is available. In this study, antiviral effect of matrine against enterovirus 71 were evaluated in vitro and in vivo. Matrine could ...

  17. The project for intercomparison of land-surface parameterization schemes (PILPS) phase 2(c) Red-Arkansas River basin experiment: 1. Experiment description and summary intercomparisons

    OpenAIRE

    Wood, EF; Lettenmaier, DP; Liang, X.; D. Lohmann; Boone, A.; Chang, S; Chen, F.; Dai, Y.; Dickinson, RE; Duan, Q; M. Ek; Gusev, YM; Habets, F.; Irannejad, P.; Koster, R

    1998-01-01

    Sixteen land-surface schemes participating in the project for the Intercomparison of Land-surface Schemes (PILPS) Phase 2(c) were run using 10 years (1979-1988) of forcing data for the Red-Arkansas River basins in the Southern Great Plains region of the United States. Forcing data (precipitation, incoming radiation and surface meteorology) and land-surface characteristics (soil and vegetation parameters) were provided to each of the participating schemes. Two groups of runs are presented. (1)...

  18. The project for intercomparison of land-surface parameterization schemes (PILPS) phase 2(c) Red-Arkansas River basin experiment: 3. Spatial and temporal analysis of water fluxes

    OpenAIRE

    Lohmann, D; Lettenmaier, DP; Liang, X.; Wood, EF; A. Boone; Chang, S.; Chen, F.; Dai, Y; Desborough, C; Dickinson, RE; Duan, Q; Ek, M.; Gusev, YM; F. Habets; P. Irannejad

    1998-01-01

    The water-balance components of 16 Soil-Vegetation Atmospheric Transfer (SVAT) schemes were evaluated by comparing predicted and observed streamflow, predicted evapotranspiration and evapotranspiration inferred from an atmospheric moisture budget analysis, and soil moisture storage changes for a seven-year period (1980-1986) using data from the Red-Arkansas River basins of the Southern Great Plains of the USA. The evaluations support the following suggestions: (a) The mean annual runoff of al...

  19. Spatio-Temporal Trends of Oak Decline and Mortality under Periodic Regional Drought in the Ozark Highlands of Arkansas and Missouri

    OpenAIRE

    Shifley, Stephen R.; Hong He; W. Keith Moser; Spetich, Martin A.; Michael K. Crosby; Zhaofei Fan; Xiuli Fan

    2012-01-01

    At the forest landscape/region level, based on annual Forest Inventory and Analysis plot data from 1999 to 2010, oak decline and mortality trends for major oak species (groups) were examined in the Ozark Highlands of Arkansas and Missouri. Oak decline has elevated cumulative mortality of red oak species to between 11 and 15 percent in terms of relative density and basal area of standing dead oak trees, respectively. These values are three to five times higher than...

  20. SR-71 - In-flight Close-up from Tanker

    Science.gov (United States)

    1994-01-01

    This extreme close-up of the SR-71B operated by NASA's Dryden Flight Research Center, Edwards, California, gives an unusual view of the twin cockpit of Dryden's SR-71B, NASA 831, and its helmeted crew members. The photo was taken from an Air Force tanker refueling the Blackbird during a 1994 flight. The Mach 3 Blackbird aircraft were loaned to NASA by the U.S. Air Force for high-speed, high-altitude aeronautical research. Capable of flying more than 2200 mph and at altitudes of over 85,000 feet, they are excellent platforms for research and experiments in aerodynamics, propulsion, structures, thermal protection materials, atmospheric studies, and sonic boom characterization. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data

  1. Taxi Arrival of Second SR-71 to Dryden

    Science.gov (United States)

    1990-01-01

    One of two initial U.S. Air Force SR-71A reconnaissance aircraft that was retired from operational service and loaned to NASA for high-speed research programs taxis in to the ramp on its arrival at NASA's Ames-Dryden Flight Research Facility (later Dryden Flight Research Center), Edwards, California in March 1990. Data from the SR-71 high speed research program will be used to aid designers of future supersonic/hypersonic aircraft and propulsion systems. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the

  2. Status of Water Levels and Selected Water-Quality Conditions in the Sparta-Memphis Aquifer in Arkansas and the Status of Water Levels in the Sparta Aquifer in Louisiana, Spring 2005

    Science.gov (United States)

    Schrader, T.P.; Jones, J.S.

    2007-01-01

    The U.S. Geological Survey in cooperation with the Arkansas Natural Resources Commission, the Arkansas Geological Commission, and the Louisiana Department of Transportation and Development has monitored water levels in the Sparta Sand of Claiborne Group and Memphis Sand of Claiborne Group since the 1920's. Ground-water withdrawals have increased while water levels have declined since monitoring was initiated. This report has been produced to describe ground-water levels in the aquifers in the Sparta Sand and Memphis Sand and provide information for the management of this valuable resource. The 2005 potentiometric-surface map of the aquifers in the Sparta Sand and Memphis Sand was constructed using water-level data collected in 333 wells in Arkansas and 120 wells in Louisiana during the spring of 2005. The highest water-level altitude measured in Arkansas was 327 feet above National Geodetic Vertical Datum of 1929 located in Grant County in the outcrop at the western boundary of the study area; the lowest water-level altitude was 189 feet below National Geodetic Vertical Datum of 1929 in Union County. The highest water-level altitude measured in Louisiana was 246 feet above National Geodetic Vertical Datum of 1929 located in Bossier Parish in the outcrop area near the western boundary of the study area; the lowest water-level altitude was 226 feet below National Geodetic Vertical Datum of 1929 in central Ouachita Parish. Three large depressions centered in Columbia, Jefferson, and Union Counties in Arkansas are the result of large withdrawals for industrial and public supplies. In Louisiana, three major pumping centers are in Ouachita, Jackson, and Lincoln Parishes. Water withdrawals from these major pumping centers primarily is used for industrial and public-supply purposes. Withdrawals from Ouachita and Lincoln Parishes and Union County, Arkansas, primarily for industrial purposes, have caused the resulting cones of depression to coalesce so that the -40 foot

  3. 7 CFR 1724.71 - Borrower contractual obligations.

    Science.gov (United States)

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES RUS Contract Forms § 1724.71 Borrower contractual obligations. (a) Loan agreement. As a condition of a loan or... obligation is contained in section 5.16 of the loan contract. To comply with the provisions of the...

  4. 42 CFR 71.52 - Turtles, tortoises, and terrapins.

    Science.gov (United States)

    2010-10-01

    ... (21 CFR 1240.62) regarding general prohibition. (g) Other permits. Permits to import certain species of turtles may be required under other Federal regulations (50 CFR parts 17 and 23) protecting such... 42 Public Health 1 2010-10-01 2010-10-01 false Turtles, tortoises, and terrapins. 71.52 Section...

  5. Urban Poor Receive RMB7.1 Billion Cash Help

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    All levels of government have teamed up to dole out more than RMB7.1 billion (US$855.42 million) during the ilrst half of this year to help more than 21 million Chinese urban residents who are living below the minimum standard of living.

  6. 7 CFR 900.71 - Hearing before Secretary.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... Practice Governing Proceedings on Petitions To Modify or To Be Exempted From Marketing Orders § 900.71... Secretary shall thereupon, after due consideration of the record, issue his final order in the...

  7. 48 CFR 915.404-4-71-6 - Fee base.

    Science.gov (United States)

    2010-10-01

    ... CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... fees on special equipment shall be based on the estimated purchase price of the equipment. (d) The fee... estimate of cost to which a percentage factor is applied to determine maximum fee allowances. The fee...

  8. 48 CFR 1852.237-71 - Pension portability.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Pension portability. 1852... 1852.237-71 Pension portability. As prescribed at 1837.110-70(b), insert the following clause: Pension Portability (JAN 1997) (a) In order for pension costs attributable to employees assigned to this contract...

  9. 36 CFR 71.9 - Establishment of recreation use fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Establishment of recreation... THE INTERIOR RECREATION FEES § 71.9 Establishment of recreation use fees. (a) Recreation use fees shall be established by all outdoor recreation administering agencies of the Department of the...

  10. 36 CFR 71.2 - Types of Federal recreation fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Types of Federal recreation... INTERIOR RECREATION FEES § 71.2 Types of Federal recreation fees. There shall be three types of Federal recreation fees: (a) Entrance fees, charged either on an annual or single-visit basis, for admission to...

  11. 21 CFR 352.71 - Light source (solar simulator).

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Light source (solar simulator). 352.71 Section 352... Light source (solar simulator). A solar simulator used for determining the SPF of a sunscreen drug... addition, a solar simulator should have no significant time-related fluctuations in radiation...

  12. 48 CFR 301.606-71 - Project Officer training.

    Science.gov (United States)

    2010-10-01

    ... a proposed project, the Program Manager or designee shall designate an individual as a Project... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Project Officer training....606-71 Project Officer training. Before an individual may perform the duties of a Project...

  13. 10 CFR 71.13 - Exemption of physicians.

    Science.gov (United States)

    2010-01-01

    ... 10 CFR part 35 or the equivalent Agreement State regulations. ... NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Exemptions... medicine is exempt from § 71.5 with respect to transport by the physician of licensed material for use...

  14. 45 CFR 98.71 - Content of reports.

    Science.gov (United States)

    2010-10-01

    ... Program Reporting Requirements § 98.71 Content of reports. (a) At a minimum, a State or territorial Lead Agency's quarterly case-level report to the Secretary, as required in § 98.70, shall include the... State or territorial Lead Agency's annual aggregate report to the Secretary, as required in §...

  15. 27 CFR 71.116 - Review by Administrator.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Review by Administrator... Review § 71.116 Review by Administrator. The Administrator, on appeal on petition for review, shall... Administrator in any proceeding in which he has engaged in investigation or prosecution, and in such event...

  16. 27 CFR 71.109 - Notice to Administrator.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Notice to Administrator... Decisions Action by the Appropriate Ttb Officer § 71.109 Notice to Administrator. When the appropriate TTB... of the decision on which it is based to the Administrator. Should such order be subsequently...

  17. 19 CFR 151.71 - Laboratory testing for clean yield.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Laboratory testing for clean yield. 151.71 Section... Laboratory testing for clean yield. (a) Test and report by Customs laboratory. The clean yield of all general... clean yield. A report of the percentage clean yield of each general sample as established by the...

  18. 48 CFR 419.201-71 - Small business coordinators.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Small business... SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 419.201-71 Small business coordinators. The head of the contracting activity (HCA) or a representative of the HCA shall designate in writing a small...

  19. 48 CFR 452.228-71 - Insurance Coverage.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Insurance Coverage. 452... FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 452.228-71 Insurance Coverage. As prescribed in 428.310, insert the following clause: Insurance Coverage (NOV 1996) Pursuant...

  20. SR-71 Mid-air Refueling with KC-135 Tanker

    Science.gov (United States)

    1995-01-01

    NASA Dryden Flight Research Center's SR-71B, tail number 831, is seen here receiving air refueling from a USAF tanker during a July, 1995 flight. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or LASRE Experiment. Another earlier project consisted of a series of flights using the SR-71 as a science camera platform for NASA's Jet Propulsion Laboratory in Pasadena, California. An upward-looking ultraviolet video camera placed in

  1. SR-71A - in Flight over Southern Sierra Nevada Mountains

    Science.gov (United States)

    1997-01-01

    NASA Dryden Flight Research Center's SR-71A, tail number 844, banks away over the Sierra Nevada mountains after air refueling from a USAF tanker during a 1997 flight. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or LASRE Experiment. Another earlier project consisted of a series of flights using the SR-71 as a science camera platform for NASA's Jet Propulsion Laboratory in Pasadena, California. An upward-looking ultraviolet

  2. SR-71B - in Flight - View from Air Force Tanker

    Science.gov (United States)

    1997-01-01

    This look-down view shows NASA 831, an SR-71B flown by Dryden Flight Research Center, Edwards, California, as it cruises over the Mojave Desert. The photo was from an Air Force refueling tanker taken on a 1997 mission. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes extending into the airstream. One of Dryden's SR-71s was used for the Linear Aerospike Rocket Engine, or LASRE Experiment. Another earlier project consisted of a series of flights using the SR-71 as a science camera platform for NASA's Jet Propulsion Laboratory in

  3. SR-71B - in flight over snow-capped mountains

    Science.gov (United States)

    1995-01-01

    Dryden's SR-71B, NASA 831, slices across the snowy southern Sierra Nevada Mountains of California after being refueled by an Air Force Flight Test Center tanker during a recent flight. The Mach 3 aircraft, on loan to NASA by the U.S. Air Force, were flown by the Dryden Flight Research Center, Edwards, California, during the decade of the 1990s as testbeds for high-speed, high-altitude aeronautical research. Capable of flying more than 2200 mph and at altitudes of over 80,000 feet, they were excellent platforms for research and experiments in aerodynamics, propulsion, structures, thermal protection materials, atmospheric studies, and sonic boom characterization. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground much like sharp thunderclaps when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startle affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It

  4. 27 CFR 71.69 - Suspension, revocation, or annulment.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Suspension, revocation, or... PROCEEDINGS Hearing Procedure Failure to Appear § 71.69 Suspension, revocation, or annulment. If on the date... Government will proceed ex parte and offer for the record sufficient evidence to make a prima facie case....

  5. 48 CFR 2452.239-71 - Information Technology Virus Security.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Information Technology... Provisions and Clauses 2452.239-71 Information Technology Virus Security. As prescribed in 2439.107(b), insert the following clause: Information Technology Virus Security (FEB 2006) (a) The contractor...

  6. 28 CFR 71.18 - Authority of the ALJ.

    Science.gov (United States)

    2010-07-01

    ... Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) IMPLEMENTATION OF THE PROVISIONS OF THE PROGRAM FRAUD CIVIL REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71... and other procedural matters; (7) Regulate the scope and timing of discovery; (8) Regulate the...

  7. 48 CFR 247.301-71 - Evaluation factor or subfactor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Evaluation factor or... Contracts 247.301-71 Evaluation factor or subfactor. For contracts that will include a significant requirement for transportation of items outside CONUS, include an evaluation factor or subfactor that...

  8. 40 CFR 141.71 - Criteria for avoiding filtration.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Criteria for avoiding filtration. 141... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all...

  9. 37 CFR 251.71 - Commencement of proceedings.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Royalty Fee Distribution Proceedings § 251.71 Commencement of proceedings. (a) Cable. In the case... year in accordance with the requirements of this subchapter. (b) Satellite carriers. In the case...

  10. Liquid Nitrogen Dewar Loading at KSC for STS-71 Flight

    Science.gov (United States)

    1995-01-01

    Liquid nitrogen dewar loading at Kennedy Space Center for STS-71 flight with Stan Koszelak (right), University of California at Riverside, adn Tamara Chinareva (left), Russian Spacecraft Coporation-Energia. The picture shows Koszelak removing the insert from the transportation dewar.

  11. 42 CFR 476.71 - QIO review requirements.

    Science.gov (United States)

    2010-10-01

    ... (CONTINUED) QUALITY IMPROVEMENT ORGANIZATIONS UTILIZATION AND QUALITY CONTROL REVIEW Review Responsibilities... care) for the palliation and management of terminal illness; (2) Whether the quality of the services... 42 Public Health 4 2010-10-01 2010-10-01 false QIO review requirements. 476.71 Section...

  12. 48 CFR 819.202-71 - Additional contracting officer responsibilities.

    Science.gov (United States)

    2010-10-01

    ... OF VETERANS AFFAIRS SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 819.202-71 Additional..., contracting officers must perform the following functions in support of the small business program: (a) Make maximum use of small business source lists. (b) Assure that small business firms are identified...

  13. 36 CFR 71.5 - Golden Eagle Passport.

    Science.gov (United States)

    2010-07-01

    ... Section 71.5 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR... of this part, shall include any passenger car, station wagon, pickup camper truck, motorcycle, or... be for sale at all Designated Entrance Fee Areas of the National Park Service, at the National...

  14. 48 CFR 2452.242-71 - Contract management system.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contract management system... 2452.242-71 Contract management system. As prescribed in 2442.1107, insert the following clause: Contract Management System (FEB 2006) (a) The contractor shall use contract management baseline...

  15. 48 CFR 1852.228-71 - Aircraft flight risks.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Aircraft flight risks. 1852... 1852.228-71 Aircraft flight risks. (a) As prescribed in 1828.311-2, insert the following clause: Aircraft Flight Risks (DEC 1988) (a) Notwithstanding any other provision of this contract...

  16. 48 CFR 852.233-71 - Alternate protest procedure.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Alternate protest... § 852.233-71 Alternate protest procedure. As prescribed in 833.106, insert the following provision: Alternate Protest Procedure (JAN 1998) As an alternative to filing a protest with the contracting...

  17. 48 CFR 733.103-71 - Filing of protest.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Filing of protest. 733.103... GENERAL CONTRACTING REQUIREMENTS PROTESTS, DISPUTES, AND APPEALS Protests 733.103-71 Filing of protest. (a) Protests must be in writing and addressed to the Contracting Officer for consideration by the...

  18. The overview of detection methods of Enterovirus 71%肠道病毒71型检测方法研究概况

    Institute of Scientific and Technical Information of China (English)

    吴海波; 郭潮潭

    2008-01-01

    Enterovirus 71(EV71)is an important pathogen of hand-foot-mouth disease(HFMD),several large outbreaks of EV71 infection have caused serious clinical symptoms and sequels,resulted in dozens of deaths.so more and more people pay attention to EV71.The paper reviews about the basic character and detection methods of EV71.%肠道病毒71型(EV71)是引起手足口病(HFMD)的重要病原,已在世界范围内引起了多次大流行,并导致了严重的并发症和后遗症,甚至死亡,引起了人们越来越多的关注.此文就EV71的基本特征和检测方法研究概况作了介绍.

  19. Neurologic Manifestations of Enterovirus 71 Infection in Korea.

    Science.gov (United States)

    Lee, Kyung Yeon; Lee, Myoung Sook; Kim, Dong Bin

    2016-04-01

    Enterovirus 71 frequently involves the central nervous system and may present with a variety of neurologic manifestations. Here, we aimed to describe the clinical features, magnetic resonance imaging (MRI) findings, and cerebrospinal fluid (CSF) profiles of patients presenting with neurologic complications of enterovirus 71 infection. We retrospectively reviewed the records of 31 pediatric patients hospitalized with acute neurologic manifestations accompanied by confirmed enterovirus 71 infection at Ulsan University Hospital between 2010 and 2014. The patients' mean age was 2.9 ± 5.5 years (range, 18 days to 12 years), and 80.6% of patients were less than 4 years old. Based on their clinical features, the patients were classified into 4 clinical groups: brainstem encephalitis (n = 21), meningitis (n = 7), encephalitis (n = 2), and acute flaccid paralysis (n = 1). The common neurologic symptoms included myoclonus (58.1%), lethargy (54.8%), irritability (54.8%), vomiting (48.4%), ataxia (38.7%), and tremor (35.5%). Twenty-five patients underwent an MRI scan; of these, 14 (56.0%) revealed the characteristic increased T2 signal intensity in the posterior region of the brainstem and bilateral cerebellar dentate nuclei. Twenty-six of 30 patients (86.7%) showed CSF pleocytosis. Thirty patients (96.8%) recovered completely without any neurologic deficits; one patient (3.2%) died due to pulmonary hemorrhage and shock. In the present study, brainstem encephalitis was the most common neurologic manifestation of enterovirus 71 infection. The characteristic clinical symptoms such as myoclonus, ataxia, and tremor in conjunction with CSF pleocytosis and brainstem lesions on MR images are pathognomonic for diagnosis of neurologic involvement by enterovirus 71 infection. PMID:27051240

  20. 41 CFR 301-71.200 - Who must review and sign travel claims?

    Science.gov (United States)

    2010-07-01

    ... travel claims? 301-71.200 Section 301-71.200 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES AGENCY RESPONSIBILITIES 71-AGENCY TRAVEL ACCOUNTABILITY REQUIREMENTS Travel Claims for Reimbursement § 301-71.200 Who must review and sign travel...

  1. New Introductions of Enterovirus 71 Subgenogroup C4 Strains, France, 2012

    OpenAIRE

    Schuffenecker, Isabelle; Henquell, Cécile; Mirand, Audrey; Coste-Burel, Marianne; Marque-Juillet, Stéphanie; Desbois, Delphine; Lagathu, Gisèle; Bornebusch, Laure; Bailly, Jean-Luc; Lina, Bruno

    2014-01-01

    In France during 2012, human enterovirus 71 (EV-A71) subgenogroup C4 strains were detected in 4 children hospitalized for neonatal fever or meningitis. Phylogenetic analysis showed novel and independent EV-A71 introductions, presumably from China, and suggested circulation of C4 strains throughout France. This observation emphasizes the need for monitoring EV-A71 infections in Europe.

  2. Potential for Optical Sensor-Based Nitrogen Fertilization in Grain Sorghum (Sorghum bicolor L. Moench) in Arkansas

    Science.gov (United States)

    Rosales Rodriguez, Kamil

    Ground-based active-optical (GBAO) crop sensors have become an effective tool to improve nitrogen (N) use efficiency and to predict yield early in the growing season, particularly for grass crops. Commercially available canopy sensors calculate the normalized difference vegetative index (NDVI) by emitting light in the red and near infrared range of the electromagnetic spectrum. The NDVI is used to evaluate vigor status and to estimate yield potential. However, few studies have been conducted to compare the performance of commercially available sensors. Therefore, a study was conducted using the most common crop canopy sensors: i) N-Tech's GreenSeeker(TM) (GS), ii) Holland Scientific's Crop Circle(TM) (CC), and iii) Minolta's SPAD-502 chlorophyll content meter (CCM). The objective of this study was to find the optimum time for sensing and compare the relative performance of the sensors in estimating the yield potential of grain sorghum (Sorghum bicolor L. Moench). Treatments included six levels of N fertilization (0, 37, 74, 111, 148, and 185 kg N/ ha), applied in a single split 20 days after planting (DAP). Treatments were arranged in a randomized complete block design with five replications, in four locations in Arkansas, during 2012 and 2013. Sensors readings at vegetative growth stages V3, 4, 5 and 6. Results from simple regression analysis showed that the V3-V4 growth stage correlated better with grain yield than readings collected and any other time. In season estimated yield (INSEY) obtained at V3 captured 41, 57, 78, and 61% of the variation in grain sorghum yield when red NDVI of GS, red NDVI of CC, red edge for CC and CCM, respectively, were used. Results from these studies suggest that the CC sensor has a better potential for in-season site-specific N application in Arkansas than the GS sensor. The GS reflectance values appear to saturate after the V3 stage, in contrast with CC values that allow for discrimination past the V3 Stage. Therefore, the red

  3. Comparing the 2000 and 2005 factors affecting the selling price of feeder cattle sold at Arkansas livestock auctions.

    Science.gov (United States)

    Troxel, T R; Barham, B L

    2007-12-01

    The objectives of the study were to determine how factors affecting the selling price of feeder calves changed from 2000 to 2005 and to examine the perception that discounts narrow or even disappear as calf supplies decrease and selling prices increase. Data from weekly Arkansas livestock auctions were collected from January 1 to December 31 in 2000 and 2005. Data included calf sex, breed type, color, muscle score, horn status, frame score, fill, condition, health, and BW. Mean selling prices for 2000 and 2005 were $92.91 +/- 15.05 and $118.32 +/- 15.13 (mean +/- SD; $/45.45 kg), respectively. Individual price observations were subtracted from the respective annual means and became the dependent variable. The selling prices for feeder calves sold in groups of 2 to 5 calves and in groups of >/= 6 calves were greater in 2005 than 2000 (P Angus x Hereford, Angus, Angus x Charolais, and Brahman (P Brahman Cross, Charolais, Charolais x Limousin, Hereford x Limousin, Limousin, Limousin x one-fourth Brahman, Longhorn, Saler and Simmental. Yellow-white face, black-white face, black, and gray feeder calves received an increase in selling price from 2000 to 2005 (P < 0.001). Although fewer horned feeder calves were sold in 2005 (P < 0.01), they received greater discounts in 2005 than 2000 (-$2.86 +/- 0.16 and -$0.51 +/- 0.09; P < 0.001). In 2005, large-framed feeder calves did not receive the premium detected in 2000, but medium-framed feeder calves in 2005 received a greater selling price compared with 2000. Feeder calves with a muscle score of 1 received a greater premium in 2005 compared with 2000 ($2.58 +/- 0.06 and $0.02 +/- 0.09, respectively; P < 0.001). Feeder calves with a muscle score of 2 were discounted in both years, but the discount in 2005 was not as great as in 2000 (P < 0.001). Full and tanked feeder calves received greater discounts in 2005 than in 2000 (P < 0.001). Discounts for fleshy and fat feeder calves were greater in 2005 than in 2000. Most factors

  4. Discovery of Gramine Derivatives That Inhibit the Early Stage of EV71 Replication in Vitro

    OpenAIRE

    Yanhong Wei; Liqiao Shi; Kaimei Wang; Manli Liu; Qingyu Yang; Ziwen Yang; Shaoyong Ke

    2014-01-01

    Enterovirus 71 (EV71) is a notable causative agent of hand, foot, and mouth disease in children, which is associated with an increased incidence of severe neurological disease and death, yet there is no specific treatment or vaccine for EV71 infections. In this study, the antiviral activity of gramine and 21 gramine derivatives against EV71 was investigated in cell-based assays. Eighteen derivatives displayed some degree of inhibitory effects against EV71, in that they could effectively inhib...

  5. Recombinant tandem multi-linear neutralizing epitopes of human enterovirus 71 elicited protective immunity in mice

    OpenAIRE

    Li, Yue-Xiang; Zhao, Hui; Cao, Rui-Yuan; Deng, Yong-Qiang; Han, Jian-Feng; Shun-ya ZHU; Ma, Jie; Liu, Long; Qin, E-De; Qin, Cheng-Feng

    2014-01-01

    Background Human Enterovirus 71 (EV71) has emerged as the leading cause of viral encephalitis in children, especially in the Asia-Pacific regions. EV71 vaccine development is of high priority at present, and neutralization antibodies have been documented to play critical roles during in vitro and in vivo protection against EV71 infection. Results In this study, a novel strategy to produce EV71 vaccine candidate based on recombinant multiple tandem linear neutralizing epitopes (mTLNE) was prop...

  6. Curcumin inhibits the replication of enterovirus 71 in vitro

    Directory of Open Access Journals (Sweden)

    Ying Qin

    2014-08-01

    Full Text Available Human enterovirus 71 (EV71 is the main causative pathogen of hand, foot, and mouth disease (HFMD in children. The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades, and no vaccine and effective antiviral medicine are available. Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections. In this study, we demonstrated that curcumin showed potent antiviral effect again EV71. In Vero cells infected with EV71, the addition of curcumin significantly suppressed the synthesis of viral RNA, the expression of viral protein, and the overall production of viral progeny. Similar with the previous reports, curcumin reduced the production of ROS induced by viral infection. However, the antioxidant property of curcumin did not contribute to its antiviral activity, since N-acetyl-l-cysteine, the potent antioxidant failed to suppress viral replication. This study also showed that extracellular signal-regulated kinase (ERK was activated by either viral infection or curcumin treatment, but the activated ERK did not interfere with the antiviral effect of curcumin, indicating ERK is not involved in the antiviral mechanism of curcumin. Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system (UPS, we found that curcumin had no impact on UPS in control cells. However, curcumin did reduce the activity of proteasomes which was increased by viral infection. In addition, the accumulation of the short-lived proteins, p53 and p21, was increased by the treatment of curcumin in EV71-infected cells. We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB, both of which are required for the formation of viral replication complex. We found that curcumin significantly reduced the level of both proteins. Moreover, the decreased expression of either GBF1 or PI4KB by the application of si

  7. Disposal of chemical agents and munitions stored at Pine Bluff Arsenal, Pine Bluff, Arkansas. Final phase 1, Environmental report

    Energy Technology Data Exchange (ETDEWEB)

    Ensminger, J.T.; Hillsman, E.L.; Johnson, R.D.; Morrisey, J.A.; Staub, W.P.; Boston, C.R.; Hunsaker, D.B.; Leibsch, E.; Rickert, L.W.; Tolbert, V.R.; Zimmerman, G.P.

    1991-09-01

    The Pine Bluff Arsenal (PBA) near Pine Bluff, Arkansas, is one of eight continental United States (CONUS) Army installations where lethal unitary chemical agents and munitions are stored and where destruction of agents and munitions is proposed under the Chemical Stockpile Disposal Program (CSDP). The chemical agent inventory at PBA consists of approximately 12%, by weight, of the total US stockpile. The destruction of the stockpile is necessary to eliminate the risk to the public from continued storage and to dispose of obsolete and leaking munitions. In 1988 the US Army issued a Final Programmatic Environmental Impact Statement (FPEIS) for the CSDP that identified on-site disposal of agents and munitions as the environmentally preferred alternative (i.e., the alternative with the least potential to cause significant adverse impacts). The purpose of this report is to examine the proposed implementation of on-site disposal at PBA in light of more recent and more detailed data than those on which the FPEIS is based. New population data were used to compute fatalities using the same computation methods and values for all other parameters as in the FPEIS. Results indicate that all alternatives are indistinguishable when the potential health impacts to the PBA community are considered. However, risks from on-site disposal are in all cases equal to or less than risks from other alternatives. Furthermore, no unique resources with the potential to prevent or delay implementation of on-site disposal at PBA have been identified.

  8. Perceived Need for Substance Abuse Treatment among Illicit Stimulant Drug Users in Rural Areas of Ohio, Arkansas, and Kentucky

    Science.gov (United States)

    Falck, Russel S.; Wang, Jichuan; Carlson, Robert G.; Krishnan, Laura L.; Leukefeld, Carl; Booth, Brenda M.

    2007-01-01

    Non-medical drug use in rural communities in the United States is a significant and growing public health threat. Understanding what motivates drug users in rural areas to seek substance abuse treatment may help in addressing the problem. Perceived need for treatment, a construct indicative of problem recognition and belief in problem solution, has been identified as an important predictor of help-seeking behavior. This cross-sectional study used data collected through face-to-face interviews to examine factors associated with perceived need for drug abuse treatment among not-in-treatment, adult, illicit stimulant drug users (n=710) in rural areas of Ohio, Kentucky, and Arkansas. More than one-quarter of the sample perceived a need for treatment. Results from a stepwise multiple regression analysis showed that white users, users with better physical and mental health status, and occasional users of methamphetamine were significantly less likely to see a need for treatment. Users with higher Addiction Severity Index composite scores for family/social problems or legal problems, and users with prior drug abuse treatment experience were significantly more likely to perceive a need for treatment. These findings have practical implications for efforts addressing substance abuse in rural areas. PMID:17604917

  9. Arkansas Nuclear One IPE: An implementation of the EPRI generic framework for IPE back end (Level 2) analysis

    International Nuclear Information System (INIS)

    In Generic Letter 88-20, the NRC asked US nuclear power plant licensees to prepare individual plant probabilistic risk studies. These studies are commonly referred to as Individual Plant Examinations (IPEs). In response to the IPE Generic Letter, Energy Operations implemented an EPRI-developed generic framework for the Level 2 analysis portion of the Arkansas Nuclear One (ANO Units 1 and 2) IPEs. It begins with grouping the Level 1 core damage accident sequences having similar characteristics into a more manageable set of plant damage states (PDSs). This serves as the interface with the Level 1 PRA, and it provides the starting point for investigating the various severe accident progression scenarios and containment response. These investigations are aided by the use of the generic containment event trees (CETs). This paper presents the implementation of the generic framework for the ANO-2 IPE and describes the integrated Level 1/2 insights on the risk-dominant sequences, potential containment vulnerabilities, and identification of likely accident management strategies. The present understanding of some severe accident phenomena is still limited. Therefore, a parametric analysis of the ANO IPE Level 2 model, as intended in the generic framework, was conducted reflecting different viewpoints in the severe accident phenomena. In particular, this allowed NRC viewpoints on various phenomenological issues, as discussed in Appendix 1 of GL 88-20, to be evaluated in a straight forward manner

  10. Genomic cloning of a heat shock cognate 71-1 gene (HSC71-1) from the hermaphroditic fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae).

    Science.gov (United States)

    Lee, Jae-Seong

    2004-02-01

    The self-fertilizing fish Rivulus marmoratus (R. marmoratus) heat shock cognate 71 (HSC71) gene was cloned and characterized recently (Park et al., 2001). Here, we report the isolation of a homologue of the R. marmoratus HSC71 gene via screening of an R. marmoratus genomic DNA library. A 12,591 bp genomic fragment was sequenced and found to contain a 2844 bp open reading frame that consisted of 8 exons and showed high similarity to the previously reported R. marmoratus HSC71 gene. The two genes differed slightly at exons 5 and 8, and intron 3. On a deduced amino acid sequence level, the two R. marmoratus HSC71 genes were highly similar (89.3% in amino acid residues). In this paper, the author presented a homologous gene (R. marmoratus HSC71-1) similar to R. marmoratus HSC71 gene. PMID:15354352

  11. Callisto - Disk temperature at 3.71-centimeter wavelength

    Science.gov (United States)

    Berge, G. L.; Muhleman, D. O.

    1975-01-01

    We observed the radio emission of Callisto with a three-element interferometer at the time of the 1973 opposition of Jupiter. Special care was taken to remove the residual, unresolved contribution from Jupiter itself in the antenna side lobes. The resulting disk temperature at a wavelength of 3.71 centimeters, assuming a radius of 2500 + or - 75 kilometers for Callisto, was 101 + or - 25 K. This temperature is much more consistent with emission from a simple dielectric sphere than the considerably higher temperatures that have been reported for wavelengths of 3.5 and 8.2 millimeters.

  12. 'n Eksegese van Jeremia 7:1–15

    Directory of Open Access Journals (Sweden)

    A. A. da Silva

    1985-01-01

    Full Text Available Jeremia 7:1–1 5 is a judgment prophecy in the style of a court verdict. Each of the five subdivisions is functional: The introduction underlines the divine authority of the judgment; the part between the introduction and the charge states the 'Law' by which the nation's transgressions would be tried; the charge stipulates the nature and gravity of the transgressions; the part between the charge and the sentence quotes a previous 'case' and the sentence stipulates that the punishment will be the same as the punishment executed against Shilo: the temple will be destroyed, and the inhabitants of Judah will be rejected.

  13. 肠道病毒71型疫苗的研究进展%Research progress of enterovirus 71 vaccine

    Institute of Scientific and Technical Information of China (English)

    李欢

    2012-01-01

    近年来,手足口病(Hand foot mouth disease,HFMD)在中国多次爆发流行,严重威胁公众健康,尤其是5岁以下的婴幼儿.而肠道病毒71型(Enterovirus 71,EV71)是引起手足口病的主要病原体之一,由于目前还无针对该疾病有效的抗病毒药物,研制疫苗是控制EV71流行最为有效的措施.目前EV71疫苗及相关研究均取得重大突破,本文就近年来关于EV71疫苗研发、动物模型等的研究进展作一综述.

  14. The developments in the diagnosis of the enteroviruses 71%肠道病毒71型实验室诊断研究进展

    Institute of Scientific and Technical Information of China (English)

    吴星

    2012-01-01

    肠道病毒71型(Enterovirus 71,EV71)为手足口病(Hand,foot and mouth disease,HFMD)和相关疾病的主要病原体,多感染婴幼儿,少数病例可以并发呼吸道感染和心肌炎、无菌性脑膜炎、脑炎、急性弛缓性麻痹等严重疾病,可致残、致死.因此EV71实验室诊断对EV71引起疾病的治疗和防控具有重要意义.本文将从核酸检测、抗体检测及其他检测等三部分对EV71的实验室诊断方法研究进展进行了综述.

  15. 肠道病毒71型分子流行病学研究进展%Progress of Reseach on Molecular Epidemiology of Enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    田波; 段海生; 荣一兵; 周世力

    2004-01-01

    肠道病毒71型(Enterovirus type 71,EV71),自1974年首次报道以来,在世界范围内引起多次爆发与流行。EV71感染主要引起患者手足口病(hand,foot and mouth disease,HFMD),在临床上与柯萨奇病毒A16(Coxsakie A16,CA16)感染所引起的手足口病难以区别,但EV71还能够引起多种与神经系统相关的疾病。近年来,EV71病毒的流

  16. Recent Progress towards Novel EV71 Anti-Therapeutics and Vaccines

    Directory of Open Access Journals (Sweden)

    Qingyong Ng

    2015-12-01

    Full Text Available Enterovirus 71 (EV71 is a group of viruses that belongs to the Picornaviridae family, which also includes viruses such as polioviruses. EV71, together with coxsackieviruses, is widely known for its association with Hand Foot Mouth Disease (HFMD, which generally affects children age five and below. Besides HFMD, EV71 can also trigger more severe and life-threatening neurological conditions such as encephalitis. Considering the lack of a vaccine and antiviral drug against EV71, together with the increasing spread of these viruses, the development of such drugs and vaccines becomes the top priority in protecting our younger generations. This article, hence, reviews some of the recent progress in the formulations of anti-therapeutics and vaccine generation for EV71, covering (i inactivated vaccines; (ii baculovirus-expressed vaccines against EV71; (iii human intravenous immunoglobulin (IVIg treatment; and (iv the use of monoclonal antibody therapy as a prevention and treatment for EV71 infections.

  17. Recent Progress towards Novel EV71 Anti-Therapeutics and Vaccines.

    Science.gov (United States)

    Ng, Qingyong; He, Fang; Kwang, Jimmy

    2015-12-01

    Enterovirus 71 (EV71) is a group of viruses that belongs to the Picornaviridae family, which also includes viruses such as polioviruses. EV71, together with coxsackieviruses, is widely known for its association with Hand Foot Mouth Disease (HFMD), which generally affects children age five and below. Besides HFMD, EV71 can also trigger more severe and life-threatening neurological conditions such as encephalitis. Considering the lack of a vaccine and antiviral drug against EV71, together with the increasing spread of these viruses, the development of such drugs and vaccines becomes the top priority in protecting our younger generations. This article, hence, reviews some of the recent progress in the formulations of anti-therapeutics and vaccine generation for EV71, covering (i) inactivated vaccines; (ii) baculovirus-expressed vaccines against EV71; (iii) human intravenous immunoglobulin (IVIg) treatment; and (iv) the use of monoclonal antibody therapy as a prevention and treatment for EV71 infections. PMID:26670245

  18. EV71 infection correlates with viral IgG preexisting at pharyngolaryngeal mucosa in children

    Institute of Scientific and Technical Information of China (English)

    Jingchang; Xue; Yaoming; Li; Xiaoyi; Xu; Jie; Yu; Hu; Yan; Huimin; Yan

    2015-01-01

    Enterovirus 71(EV71) infection causes severe central nervous system damage, particularly for children under the age of 5 years old, which remains a major public health burden worldwide. Clinical data released that children may be repeatedly infected by different members in enterovirus and get even worsen. Mucosa, especially epithelium of alimentary canal, was considered the primary site of EV71 infection. It has been elusive whether the preexsiting viral antibody in mucosa plays a role in EV71 infection. To answer this question, we respectively measured viral antibody response and EV71 RNA copy number of one hundred throat swab specimens from clinically confirmed EV71-infected children. The results released that low-level of mucosal Ig G antibody against EV71 broadly existed in young population. More importantly, it further elucidated that the children with mucosal preexsiting EV71 Ig G were prone to be infected, which suggested a former viral Ig G mediated enhancement of viral infection in vivo.

  19. Progress in research on inactivated whole virus vaccine against enterovirus 71%EV71全病毒灭活疫苗的研究进展

    Institute of Scientific and Technical Information of China (English)

    姚昕; 毛群颖

    2012-01-01

    Hand, foot and mouth disease(HFMD) has been circulated in China and has become a serious public health problem. Enterovirus 71 (EV71) is emerged as a significant pathogen causing severe and death cases. Various EV71 vaccines are in development for controlling EV71 infection, of which inactivated vaccines are developed rapidly. At present, phase HI clinical trials will be performed on the inactivated whole virus vaccines against EV71 manufactured by three manufacturers in China. This review attempts to give an overview of the progress and quality control of inactivated whole virus vaccine against EV71.%近年来手足口病(Hand,foot and mouth disease,HFMD)在我国持续流行,已经成为危害儿童健康的严重疾病.肠道病毒71型(Enterovirus 71,EV71)感染是引起HFMD病例和婴儿重症死亡的主要病因.为控制EV71感染的流行,国内外均在开展不同种类EV71疫苗的研发,其中以灭活疫苗的进展最快,目前国内3家EV71全病毒灭活疫苗即将进入Ⅲ期临床试验阶段.本文就全病毒灭活疫苗的研发进展及质量控制和评价要点作一综述.

  20. Surveillance of the enterovirus type 71 in Shenzhen in 2000%2000年深圳市肠道病毒71型的监测

    Institute of Scientific and Technical Information of China (English)

    何雅青; 杨洪; 丰素娟; 严志平

    2003-01-01

    @@ 肠道病毒71型(enterovirus type 71; EV 71)的感染可导致多种临床症状,EV 71可以引起发热性疾病、手足口病、疱疹性咽峡炎、无菌性脑膜炎、脑炎、脑干脑炎、肺水肿、脊髓灰质炎样的麻痹性疾病等多种疾病,甚至导致死亡。

  1. Biological characteristics and research progress of enterovirus 71%肠道病毒71型的生物学特征及研究进展

    Institute of Scientific and Technical Information of China (English)

    戈小琴; 李鸿钧

    2010-01-01

    In recent years, the prevalence of enterovirus 71 (EV71) shows rising trend in Asia-Pacific area. The hand, foot and mouth disease caused by EV71 attracts widespread attention, and becomes a large challenge for preventive medicine and basic scientific research. This paper reviews research progress in biological characteristics, epidemiology, pathogenesis, clinical and laboratory diagnosis, prevention, and treatment of EV71.%近年来,肠道病毒71型(enterovirus 71,EV71)的流行在亚太地区呈逐渐上升趋势.EV71导致的手足口病已引起社会各界广泛关注,并成为医学预防和基础科研的挑战.此文就EV71的生物学特性、流行病学、致病机制、临床和实验室诊断、预防及治疗等方面的研究进展作一综述.

  2. RT-PCR法在快速检测肠道病毒71型(EV71)核酸中应用

    Institute of Scientific and Technical Information of China (English)

    李国兰; 郭玲; 李治悦; 卜戈; 于昌军; 张威; 杨帆; 李建国; 王伟

    2008-01-01

    @@ 2008年4月至5月发生在安徽阜阳的手足口病流行其病原已经确定为肠道病毒71型(Enterovirus 71,EV71).EV71属于小RNA病毒科(Picornaradae)肠道病毒属(Enterovirus),为一种单正链RNA病毒,病毒核酸含有大约7411个核苷酸.病毒颗粒大致呈球形,无包膜和突起,直径24~30 nm.

  3. Progress in Research on Enterovirus 71 Vaccine%肠道病毒71型疫苗的研发现状及进展

    Institute of Scientific and Technical Information of China (English)

    姚昕; 毛群颖

    2011-01-01

    Hand foot mouth disease (HFMD) has been epidemic in China in recent years. Severe and fatal cases caused by enterovirus 71 (EV71) has become the serious public healthy problem. The only effective measure to control HFMD is developing EV71 vaccine. At present, the vaccine is in developing in several manufacturers in China. This review attempts to give an overview of the necessity, feasibility as well as problem and progress in development of EV71 vaccine.%近年来,手足口病(Hand foot mouth disease,HFMD)在我国呈持续流行态势.其中,肠道病毒71型(Enterovirus 71,EV71)感染引起的严重和死亡病例已成为政府和社会高度关注的公共卫生问题.研发疫苗是控制该病流行的唯一有效措施.目前,我国已有多个单位开展了EV71疫苗的研发工作,本文就EV71疫苗研发的必要性、可行性、研发的难点及研发进展作一综述.

  4. 肠道病毒71型致病机制研究进展%Advances in the research of pathogenesis of enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    鲁卫卫; 郭会杰

    2013-01-01

    Enterovirus 71 (EV71) is one of the major pathogens of hand,foot and mouth disease,which can cause a variety of nervous system-related diseases with high disability and mortality rates.This review summarizes the recent research progress of the immune responses against EV71 and pathogenesis of EV71 infection.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病的主要病原体之一,其感染可导致多种与神经系统相关的疾病,致残率及病死率较高.目前,关于其免疫机制和致病机制尚未完全清楚.此文阐述了机体对EV71的免疫应答以及EV71感染的致病机制的最新研究进展.

  5. Progress on the research and development of inactivated EV71 whole-virus vaccines

    OpenAIRE

    Liang, Zheng-Lun; Mao, Qun-Ying; Wang, Yi-Ping; Zhu, Feng-cai; Li, Jing-Xin; Xin YAO; Gao, Fan; Wu, Xing; Xu, Miao; Wang, Jun-Zhi

    2013-01-01

    The prevalence of diseases caused by EV71 infection has become a serious public health problem in the Western Pacific region. Due to a lack of effective treatment options, controlling EV71 epidemics has mainly focused on the research and development (R&D) of EV71 vaccines. Thus far, five organizations have completed pre-clinical studies focused on the development of inactivated EV71 whole-virus vaccines, including vaccine strain screening, process optimization, safety and immunogenicity evalu...

  6. 肠道病毒71型临床研究进展

    Institute of Scientific and Technical Information of China (English)

    赵红; 欧蔚妮; 闫杰; 谢雯

    2008-01-01

    @@ 肠道病毒71型(enterovirus 71, EV71)为小RNA病毒科肠道病毒属成员.EV71于1969年首次从美国加利福尼亚州一名患有中枢神经系统疾病婴儿的粪便标本中分离出后[1],其流行范围遍及全球.

  7. Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein

    OpenAIRE

    Kiener Tanja K; Jia Qiang; Lim Xiao; He Fang; Meng Tao; Kwong Chow Vincent; Kwang Jimmy

    2012-01-01

    Abstract Background Enterovirus 71 (EV71) has emerged as a major causative agent of hand, foot and mouth disease in the Asia-Pacific region over the last decade. Hand, foot and mouth disease can be caused by different etiological agents from the enterovirus family, mainly EV71 and coxsackieviruses, which are genetically closely related. Nevertheless, infection with EV71 may occasionally lead to high fever, neurologic complications and the emergence of a rapidly fatal syndrome of pulmonary ede...

  8. New introductions of enterovirus 71 subgenogroup C4 strains, France, 2012.

    Science.gov (United States)

    Schuffenecker, Isabelle; Henquell, Cécile; Mirand, Audrey; Coste-Burel, Marianne; Marque-Juillet, Stéphanie; Desbois, Delphine; Lagathu, Gisèle; Bornebusch, Laure; Bailly, Jean-Luc; Lina, Bruno

    2014-08-01

    In France during 2012, human enterovirus 71 (EV-A71) subgenogroup C4 strains were detected in 4 children hospitalized for neonatal fever or meningitis. Phylogenetic analysis showed novel and independent EV-A71 introductions, presumably from China, and suggested circulation of C4 strains throughout France. This observation emphasizes the need for monitoring EV-A71 infections in Europe. PMID:25061698

  9. Global absolut gravity reference system as replacement of IGSN 71

    Science.gov (United States)

    Wilmes, Herbert; Wziontek, Hartmut; Falk, Reinhard

    2015-04-01

    The determination of precise gravity field parameters is of great importance in a period in which earth sciences are achieving the necessary accuracy to monitor and document global change processes. This is the reason why experts from geodesy and metrology joined in a successful cooperation to make absolute gravity observations traceable to SI quantities, to improve the metrological kilogram definition and to monitor mass movements and smallest height changes for geodetic and geophysical applications. The international gravity datum is still defined by the International Gravity Standardization Net adopted in 1971 (IGSN 71). The network is based upon pendulum and spring gravimeter observations taken in the 1950s and 60s supported by the early free fall absolute gravimeters. Its gravity values agreed in every case to better than 0.1 mGal. Today, more than 100 absolute gravimeters are in use worldwide. The series of repeated international comparisons confirms the traceability of absolute gravity measurements to SI quantities and confirm the degree of equivalence of the gravimeters in the order of a few µGal. For applications in geosciences where e.g. gravity changes over time need to be analyzed, the temporal stability of an absolute gravimeter is most important. Therefore, the proposition is made to replace the IGSN 71 by an up-to-date gravity reference system which is based upon repeated absolute gravimeter comparisons and a global network of well controlled gravity reference stations.

  10. 48 CFR 1852.247-71 - Protection of the Florida manatee.

    Science.gov (United States)

    2010-10-01

    ... manatee. 1852.247-71 Section 1852.247-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND... and Clauses 1852.247-71 Protection of the Florida manatee. As prescribed in 1847.7001, insert the following clause: Protection of the Florida Manatee (MAR 1989) (a) Pursuant to the Endangered Species Act...

  11. 7 CFR 201.71 - Establishing the source of all classes of certified seed.

    Science.gov (United States)

    2010-01-01

    ... seed. 201.71 Section 201.71 Agriculture Regulations of the Department of Agriculture (Continued... (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Certified Seed § 201.71 Establishing the source of all classes of certified seed. The certifying agency shall have evidence of the class and source...

  12. 41 CFR 301-71.101 - What travel may we authorize?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What travel may we authorize? 301-71.101 Section 301-71.101 Public Contracts and Property Management Federal Travel Regulation... REQUIREMENTS Travel Authorization § 301-71.101 What travel may we authorize? You may authorize only...

  13. 48 CFR 352.237-71 - Crime Control Act-reporting of child abuse.

    Science.gov (United States)

    2010-10-01

    ...-reporting of child abuse. 352.237-71 Section 352.237-71 Federal Acquisition Regulations System HEALTH AND... Clauses 352.237-71 Crime Control Act—reporting of child abuse. As prescribed in 337.103-70(b), the Contracting Officer shall insert the following clause: Crime Control Act of 1990—Reporting of Child...

  14. Cytometric evaluation of transferrin receptor 1 (CD71 in childhood acute lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Anna Płoszyńska

    2012-07-01

    Full Text Available Transferrin receptor 1 (CD71 is a transmembrane glycoprotein responsible for cellular iron uptake. Higher expression of CD71 has been identified as a negative prognostic marker for numerous solid tumor types and for some lymphomas. The aim of this study was to evaluate CD71 expression on acute lymphoblastic leukemia (ALL cells and to follow its possible clinical correlations. Sixty one patients, aged 1–17 years and diagnosed with ALL, were enrolled in the study. CD71 expression was analyzed on the bone marrow blastic cells by flow cytometry. CD71 expression on the  eukemic blasts was diversified; in most patients, all blastic cells showed expression of CD71, but levels of expression varied. CD71 expression was statistically higher on T-lineage leukemias. Within the B lineage ALL, a significant difference in CD71 expression existed between precursor B ALL and mature B-ALL, which showed higher CD71 expression. CD71 expression positively correlated with Hgb concentration at diagnosis. Initial risk group assessment and therapy response were not correlated with CD71 expression, although disease free and overall survival times tended to be shorter in patients with B-lineage leukemias with initial high CD71 expression.

  15. 48 CFR 945.603-71 - Disposal of radioactively contaminated personal property.

    Science.gov (United States)

    2010-10-01

    ... Contractor Inventory 945.603-71 Disposal of radioactively contaminated personal property. Special procedures regarding the disposal of radioactively contaminated property may be found at 41 CFR 109-45.50. ... contaminated personal property. 945.603-71 Section 945.603-71 Federal Acquisition Regulations System...

  16. 42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false African rodents and other animals that may carry the monkeypox virus. 71.56 Section 71.56 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES QUARANTINE, INSPECTION, LICENSING FOREIGN QUARANTINE Importations § 71.56 African...

  17. 48 CFR 215.404-71-3 - Contract type risk and working capital adjustment.

    Science.gov (United States)

    2010-10-01

    ... working capital adjustment. 215.404-71-3 Section 215.404-71-3 Federal Acquisition Regulations System... CONTRACTING BY NEGOTIATION Contract Pricing 215.404-71-3 Contract type risk and working capital adjustment. (a... varying contract types. The working capital adjustment is an adjustment added to the profit objective...

  18. 48 CFR 652.239-71 - Security Requirements for Unclassified Information Technology Resources.

    Science.gov (United States)

    2010-10-01

    ... Unclassified Information Technology Resources. 652.239-71 Section 652.239-71 Federal Acquisition Regulations... Provisions and Clauses 652.239-71 Security Requirements for Unclassified Information Technology Resources. As... Technology Resources (SEP 2007) (a) General. The Contractor shall be responsible for information...

  19. 9 CFR 71.19 - Identification of swine in interstate commerce.

    Science.gov (United States)

    2010-01-01

    ... commerce, except at the time of slaughter as provided in 9 CFR 309.16(e). (g) Swine moving interstate... commerce. 71.19 Section 71.19 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE... GENERAL PROVISIONS § 71.19 Identification of swine in interstate commerce. (a)(1) Except as provided...

  20. 48 CFR 504.602-71 - Federal Procurement Data System-Public access to data.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Federal Procurement Data System-Public access to data. 504.602-71 Section 504.602-71 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION GENERAL ADMINISTRATIVE MATTERS Contract Reporting 504.602-71 Federal Procurement Data System—Public access to data....

  1. 28 CFR 71.40 - Stays ordered by the Department of Justice.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Stays ordered by the Department of Justice. 71.40 Section 71.40 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) IMPLEMENTATION OF... the Department of Justice § 71.40 Stays ordered by the Department of Justice. If at any time...

  2. Evolutionary trajectory of the VP1 gene of human enterovirus 71 genogroup B and C viruses

    NARCIS (Netherlands)

    S.M.G. van der Sanden (Sabine); H.G.A.M. van der Avoort (Harrie); P. Lemey (Philippe); G. Uslu (Gökhan); M.P.G. Koopmans D.V.M. (Marion)

    2010-01-01

    textabstractFrom 1963 to 1986, human enterovirus 71 (HEV71) infections in the Netherlands were successively caused by viruses of subgenogroups B0, B1 and B2. A genogroup shift occurred in 1987, after which viruses of subgenogroups C1 and C2 were detected exclusively. This is in line with HEV71 typin

  3. Expression and Purification of Enterovirus Type 71 Polyprotein P1 using Pichia pastoris system

    Institute of Scientific and Technical Information of China (English)

    Xue Han; Xiaoling Ying; Hao Huang; Shili Zhou; Qi Huang

    2012-01-01

    Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year; However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 pretein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.

  4. 50 CFR 71.1 - Opening of national fish hatchery areas to hunting.

    Science.gov (United States)

    2010-10-01

    ... hunting. 71.1 Section 71.1 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MANAGEMENT OF FISHERIES CONSERVATION AREAS HUNTING AND FISHING ON NATIONAL FISH HATCHERY AREAS Hunting § 71.1 Opening of national fish hatchery areas to hunting. National...

  5. 48 CFR 1352.233-71 - GAO and Court of Federal Claims protests.

    Science.gov (United States)

    2010-10-01

    ....233-71 GAO and Court of Federal Claims protests. As prescribed in 48 CFR 1333.104-70(a), insert the following provision: GAO and Court of Federal Claims Protests (APR 2010) (a) A protest may be filed with... Claims protests. 1352.233-71 Section 1352.233-71 Federal Acquisition Regulations System DEPARTMENT...

  6. 肠道病毒71型灭活疫苗相关研究现状%Current status of research of inactivated enterovirus 71 vaccines

    Institute of Scientific and Technical Information of China (English)

    杨二霞

    2013-01-01

    Hand-foot-and-mouth disease (HFMD) is a life-threatening infectious disease of children under 5 years old.In recent years,major outbreaks of HFMD caused by enterovirus 71 (EV71) have emerged in the Asia-Pacific region,and have resulted in millions of cases and hundreds of deaths each year in China since 2008.Because EV71 is one of the main pathogens of HFMD resulting in severe diseases and death,the pathogenesis of HFMD is still unclear,and no effective therapeutic drugs for EV71 infection are currently available,development of vaccines against EV71 is urgently needed.This review summarizes research progress of inactivated EV71 vaccines.%手足口病是一种威胁5岁以下儿童生命健康的传染病,近年来已在亚太地区造成了多次上百万患者发病的大规模暴发,仅在中国大陆,自2008年以来,每年发病人数就超过百万,死亡数百人.然而,由于肠道病毒71型(enterovirus 71,EV71)是导致重症和死亡病例的主要病原体,而其致病机制尚不清楚,目前尚无有效的治疗性药物,因此EV71疫苗研究成为迫切需要解决的问题.此文就EV71灭活疫苗研究进展进行综述.

  7. Interaction of Human PD-L1 and B7-1

    OpenAIRE

    Butte, Manish J.; Pena-Cruz, Victor; Kim, Mi-Jung; Freeman, Gordon J; Sharpe, Arlene H.

    2008-01-01

    Numerous studies have pointed to the role of Programmed Death-1 Ligand 1 (PD-L1) in regulating tolerance, chronic infection, and tumor immunity. Recently, we have identified murine B7-1 as a new binding partner for murine PD-L1. Human and mouse B7-1 share only 46% identity, leading us to question whether human B7-1 and PD-L1 can participate in a similar interaction. Here we show that human B7-1 can interact with human PD-L1 with affinity greater than that of B7-1 with CD28, but less than that...

  8. Enterovirus 71 Disrupts Interferon Signaling by Reducing the Level of Interferon Receptor 1

    OpenAIRE

    Lu, Jing; Yi, Lina; Zhao, Jin; Yu, Jun; Chen, Ying; Lin, Marie C.; Kung, Hsiang-Fu; He, Ming-liang

    2012-01-01

    The recent outbreak of enterovirus 71 (EV71) infected millions of children and caused over 1,000 deaths. To date, neither an effective vaccine nor antiviral treatment is available for EV71 infection. Interferons (IFNs) have been successfully applied to treat patients with hepatitis B and C viral infections for decades but have failed to treat EV71 infections. Here, we provide the evidence that EV71 antagonizes type I IFN signaling by reducing the level of interferon receptor 1 (IFNAR1). We sh...

  9. Genetic and antigenic characterization of enterovirus 71 in Ho Chi Minh City, Vietnam, 2011.

    Directory of Open Access Journals (Sweden)

    Le Phan Kim Thoa

    Full Text Available Enterovirus 71 (EV71 frequently causes fatal infections in young children in Asia. In 2011, EV71 epidemics occurred in southern Vietnam. We conducted genetic and antigenic analysis of the EV71 isolates and found that 94% of them were genotype C4a related to two lineages circulating in China and 6% were genotype C5 which have circulated in Vietnam since 2003. Antigenic variants were not detected. EV71 vaccines are being developed. Longitudinal enterovirus surveillance data are critical to formulate vaccination policy in Vietnam.

  10. Rupintrivir is a promising candidate for treating severe cases of Enterovirus-71 infection

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    AIM:To evaluate the suitability of rupintrivir against Enterovirus 71(EV71)induced severe clinical symptoms using computational methods. METHODS:The structure of EV71 3C protease was predicted by homology modeling.The binding free energies between rupintrivir and EV71 3C and human rhinovirus 3C protease were computed by molecular dynamics and molecular mechanics Poisson-Boltzmann/ surface area and molecular mechanics generalized-born/ surface area methods.EV71 3C fragments obtained from clinical samples col...

  11. 71Ga Chemical Shielding and Quadrupole Coupling Tensors of the Garnet Y(3)Ga(5)O(12) from Single-Crystal (71)Ga NMR

    DEFF Research Database (Denmark)

    Vosegaard, Thomas; Massiot, Dominique; Gautier, Nathalie;

    1997-01-01

    A single-crystal (71)Ga NMR study of the garnet Y(3)Ga(5)O(12) (YGG) has resulted in the determination of the first chemical shielding tensors reported for the (71)Ga quadrupole. The single-crystal spectra are analyzed in terms of the combined effect of quadrupole coupling and chemical shielding ...

  12. Determinants of EV71 immunogenicity and protection against lethal challenge in a mouse model.

    Science.gov (United States)

    Chang, Junliang; Li, Jingliang; Wei, Wei; Liu, Xin; Liu, Guanchen; Yang, Jiaxin; Zhang, Wenyan; Yu, Xiao-Fang

    2015-07-01

    Circulating enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) is a major public health problem in the Asian-Pacific region. An EV71 vaccine for HFMD prevention is currently being developed. However, viral determinants that could influence the vaccine's efficacy have not been well characterized. In this study, we isolated and characterized several EV71 strains that are currently circulating in northern and southern China. We determined that VP1 variation is a major determinant of EV71 immunogenicity. A single amino acid variation in VP1 can lead to significant differences in the breadth and potency of immune responses against primary EV71 isolates as well as the sensitivity of EV71 to heterologous neutralizing antibody responses. We also identified EV71 strains that could induce potent immunogenic and cross-neutralizing antibody responses against diverse EV71 strains. Furthermore, these neutralizing antibodies could protect neonatal mice from lethal dose challenge with various circulating EV71 viruses. Our study provides useful information for EV71 vaccine development and evaluation. PMID:26025091

  13. Immunization of N terminus of enterovirus 71 VP4 elicits cross-protective antibody responses

    Science.gov (United States)

    2013-01-01

    Background Enterovirus 71 (EV71) is major cause of hand, foot and mouth disease. Large epidemics of EV71 infection have been recently reported in the Asian-Pacific region. Currently, no vaccine is available to prevent EV71 infection. Results The peptide (VP4N20) consisting of the first 20 amino acids at the N-terminal of VP4 of EV71 genotype C4 were fused to hepatitis B core (HBcAg) protein. Expression of fusion proteins in E. coli resulted in the formation of chimeric virus-like particles (VLPs). Mice immunized with the chimeric VLPs elicited anti-VP4N20 antibody response. In vitro microneutralization experiments showed that anti-chimeric VLPs sera were able to neutralize not only EV71 of genotype C4 but also EV71 of genotype A. Neonatal mice model confirmed the neutralizing ability of anti-chimeric VLPs sera. Eiptope mapping led to the identification of a “core sequence” responsible for antibody recognition within the peptide. Conclusions Immunization of chimeric VLPs is able to elicit antibodies displaying a broad neutralizing activity against different genotypes of EV71 in vitro. The “core sequence” of EV71-VP4 is highly conserved across EV71 genotypes. The chimeric VLPs have a great potential to be a novel vaccine candidate with a broad cross-protection against different EV71 genotypes. PMID:24320792

  14. Sialylated glycans as receptor and inhibitor of enterovirus 71 infection to DLD-1 intestinal cells

    Directory of Open Access Journals (Sweden)

    Yang Kuender D

    2009-09-01

    Full Text Available Abstract Background Many viruses recognize specific sugar residues, particularly sulfated or sialylated glycans, as the infection receptors. A change of sialic acid (2-6-linked galactose (SA-α2,6Gal to SA-α2,3Gal determines the receptor for avian flu infection. The receptor for enterovirus 71 (EV71 infection that frequently causes fatal encephalitis in Asian children remains unclear. Currently, there is no effective vaccine or anti-virus agent for EV71 infection. Using DLD-1 intestinal cells, this study investigated whether SA-linked glycan on DLD-1 intestinal cells was a receptor for EV71, and whether natural SA-linked sugars from human milk could block EV71 infection. Results EV71 specifically infected DLD-1 intestinal cells but not K562 myeloid cells. Depletion of O-linked glycans or glycolipids, but not N-linked glycans, significantly decreased EV71 infection of DLD-1 cells. Pretreatment of DLD-1 cells with sialidase (10 mU, 2 hours significantly reduced 20-fold EV71 replication (p Conclusion This is the first in the literature to demonstrate that EV71 uses SA-linked glycans as receptors for infection, and natural SA-linked glycans from human milk can protect intestinal cells from EV71 infection. Further studies will test how a SA-containing glycan can prevent EV71 in the future.

  15. The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection

    Directory of Open Access Journals (Sweden)

    Yong-Zhe Zhu

    2015-02-01

    Full Text Available Background/Aims: Although it has been widely accepted that Enterovirus 71 (EV71 enters permissive cells via receptor-mediated endocytosis, the details of entry mechanism for EV71 still need more exploration. This study aimed to investigate the role of lipid rafts in the early stage of EV71 Infection. Methods: The effect of cholesterol depletion or addition of exogenous cholesterol was detected by immunofluorescence assays and quantitative real-time PCR. Effects of cholesterol depletion on the association of EV71 with lipid rafts were determined by flow cytometry and co-immunoprecipitation assays. Localization and internalization of EV71 and its receptor were assayed by confocal microscpoy and sucrose gradient analysis. The impact of cholesterol on the activation of phosphoinositide 3'-kinase/Akt signaling pathway during initial virus infection was analyzed by Western-blotting. Results: Disruption of membrane cholesterol by a pharmacological agent resulted in a significant reduction in the infectivity of EV71. The inhibitory effect could be reversed by the addition of exogenous cholesterol. Cholesterol depletion post-infection did not affect EV71 infection. While virus bound equally to cholesterol-depleted cells, EV71 particles failed to be internalized by cholesterol-depleted cells. EV71 capsid protein co-localized with cholera toxin B, a lipid-raft-dependent internalization marker. Conclusion: Lipid rafts play a critical role in virus endocytosis and in the activation of PI3K/Akt signaling pathway in the early stage of EV71 infection.

  16. User's guide to ESME v. 7.1

    International Nuclear Information System (INIS)

    ESME is a computer program to calculate the evolution of a distribution of particles in energy and azimuth as it is acted upon by the radiofrequency system of a proton synchrotron. It provides for the modeling of multiple rf systems, feedback control, spacecharge, and many of the effects of longitudinal coupling impedance. The capabilities of the program are described, and the requirements for input data are specified in sufficient detail to permit significant calculations by an uninitiated user. The program is currently at version 7.1 and extensively modified since the previous user documentation. Fundamental enhancements make version 6 data unusable, but nearly all facilities of the earlier version have been retained and input data is similar. Also described is a VAX-based code management convention which has been established with a view to maintaining functional equivalence in versions used on different computers. 13 refs

  17. The isobaric multiplet mass equation for A≤71 revisited

    International Nuclear Information System (INIS)

    Accurate mass determination of short-lived nuclides by Penning-trap spectrometers and progress in the spectroscopy of proton-rich nuclei have triggered renewed interest in the isobaric multiplet mass equation (IMME). The energy levels of the members of T=1/2,1,3/2, and 2 multiplets and the coefficients of the IMME are tabulated for A≤71. The new compilation is based on the most recent mass evaluation (AME2011) and it includes the experimental results on energies of the states evaluated up to end of 2011. Taking into account the error bars, a significant deviation from the quadratic form of the IMME for the A=9,35 quartets and the A=32 quintet is observed

  18. Phage Display-Derived Cross-Reactive Neutralizing Antibody against Enterovirus 71 and Coxsackievirus A16.

    Science.gov (United States)

    Zhang, Xiao; Sun, Chunyun; Xiao, Xiangqian; Pang, Lin; Shen, Sisi; Zhang, Jie; Cen, Shan; Yang, Burton B; Huang, Yuming; Sheng, Wang; Zeng, Yi

    2016-01-01

    Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the Picornaviridae family and are considered the main causative agents of hand, foot and mouth disease (HFMD). In recent decades large HFMD outbreaks caused by EV71 and CVA16 have become significant public health concerns in the Asia-Pacific region. Vaccines and antiviral drugs are unavailable to prevent EV71 and CVA16 infection. In the current study, a chimeric antibody targeting a highly conserved peptide in the EV71 VP4 protein was isolated by using a phage display technique. The antibody showed cross-neutralizing capability against EV71 and CVA16 in vitro. The results suggest that this phage display-derived antibody will have great potential as a broad neutralizing antibody against EV71 and CVA16 after affinity maturation and humanization. PMID:26073737

  19. Ambient conditions and fate and transport simulations of dissolved solids, chloride, and sulfate in Beaver Lake, Arkansas, 2006--10

    Science.gov (United States)

    Green, W. Reed

    2013-01-01

    Beaver Lake is a large, deep-storage reservoir located in the upper White River Basin in northwestern Arkansas, and was completed in 1963 for the purposes of flood control, hydroelectric power, and water supply. Beaver Lake is affected by point and nonpoint sources of minerals, nutrients, and sediments. The City of Fayetteville discharges about half of its sewage effluent into the White River immediately upstream from the backwater of the reservoir. The City of West Fork discharges its sewage effluent into the West Fork of the White River, and the City of Huntsville discharges its sewage effluent into a tributary of War Eagle Creek. A study was conducted to describe the ambient conditions and fate and transport of dissolved solids, chloride, and sulfate concentrations in Beaver Lake. Dissolved solids, chloride, and sulfate are components of wastewater discharged into Beaver Lake and a major concern of the drinking water utilities that use Beaver Lake as their source. A two-dimensional model of hydrodynamics and water quality was calibrated to include simulations of dissolved solids, chloride, and sulfate for the period January 2006 through December 2010. Estimated daily dissolved solids, chloride, and sulfate loads were increased in the White River and War Eagle Creek tributaries, individually and the two tributaries together, by 1.2, 1.5, 2.0, 5.0, and 10.0 times the baseline conditions to examine fate and transport of these constituents through time at seven locations (segments) in the reservoir, from upstream to downstream in Beaver Lake. Fifteen dissolved solids, chloride, and sulfate fate and transport scenarios were compared to the baseline simulation at each of the seven downstream locations in the reservoir, both 2 meters (m) below the surface and 2 m above the bottom. Concentrations were greater in the reservoir at model segments closer to where the tributaries entered the reservoir. Concentrations resulting from the increase in loading became more diluted

  20. Enterovirus 71 Uses Cell Surface Heparan Sulfate Glycosaminoglycan as an Attachment Receptor

    Science.gov (United States)

    Tan, Chee Wah; Poh, Chit Laa; Sam, I-Ching

    2013-01-01

    Enterovirus 71 (EV-71) infections are usually associated with mild hand, foot, and mouth disease in young children but have been reported to cause severe neurological complications with high mortality rates. To date, four EV-71 receptors have been identified, but inhibition of these receptors by antagonists did not completely abolish EV-71 infection, implying that there is an as yet undiscovered receptor(s). Since EV-71 has a wide range of tissue tropisms, we hypothesize that EV-71 infections may be facilitated by using receptors that are widely expressed in all cell types, such as heparan sulfate. In this study, heparin, polysulfated dextran sulfate, and suramin were found to significantly prevent EV-71 infection. Heparin inhibited infection by all the EV-71 strains tested, including those with a single-passage history. Neutralization of the cell surface anionic charge by polycationic poly-d-lysine and blockage of heparan sulfate by an anti-heparan sulfate peptide also inhibited EV-71 infection. Interference with heparan sulfate biosynthesis either by sodium chlorate treatment or through transient knockdown of N-deacetylase/N-sulfotransferase-1 and exostosin-1 expression reduced EV-71 infection in RD cells. Enzymatic removal of cell surface heparan sulfate by heparinase I/II/III inhibited EV-71 infection. Furthermore, the level of EV-71 attachment to CHO cell lines that are variably deficient in cell surface glycosaminoglycans was significantly lower than that to wild-type CHO cells. Direct binding of EV-71 particles to heparin-Sepharose columns under physiological salt conditions was demonstrated. We conclude that EV-71 infection requires initial binding to heparan sulfate as an attachment receptor. PMID:23097443

  1. Geochemical and isotopic variations in shallow groundwater in areas of the Fayetteville Shale development, north-central Arkansas

    International Nuclear Information System (INIS)

    Highlights: • No evidence for shallow groundwater contamination in Fayetteville Shale, Arkansas. • Methane in groundwater is low and likely associated with shallow aquifer processes. • No relationship between methane and salinity in groundwater and shale-gas wells. • δ13CCH4 and δ13CDIC suggest biogenic origin for dissolved methane. • Water-aquifer rock interaction controls majority of water chemistry. - Abstract: Exploration of unconventional natural gas reservoirs such as impermeable shale basins through the use of horizontal drilling and hydraulic fracturing has changed the energy landscape in the USA providing a vast new energy source. The accelerated production of natural gas has triggered a debate concerning the safety and possible environmental impacts of these operations. This study investigates one of the critical aspects of the environmental effects; the possible degradation of water quality in shallow aquifers overlying producing shale formations. The geochemistry of domestic groundwater wells was investigated in aquifers overlying the Fayetteville Shale in north-central Arkansas, where approximately 4000 wells have been drilled since 2004 to extract unconventional natural gas. Monitoring was performed on 127 drinking water wells and the geochemistry of major ions, trace metals, CH4 gas content and its C isotopes (δ13CCH4), and select isotope tracers (δ11B, 87Sr/86Sr, δ2H, δ18O, δ13CDIC) compared to the composition of flowback-water samples directly from Fayetteville Shale gas wells. Dissolved CH4 was detected in 63% of the drinking-water wells (32 of 51 samples), but only six wells exceeded concentrations of 0.5 mg CH4/L. The δ13CCH4 of dissolved CH4 ranged from −42.3‰ to −74.7‰, with the most negative values characteristic of a biogenic source also associated with the highest observed CH4 concentrations, with a possible minor contribution of trace amounts of thermogenic CH4. The majority of these values are distinct from the

  2. Detailed lithologic log of the Dow Chemical #1 B.L. Garrigan Drill Hole, Mississippi County, Arkansas

    Science.gov (United States)

    Collins, Donley S.; Skipp, Gary L.

    1995-01-01

    The geology and tectonic setting of the New Madrid region in southeastern Missouri has received considerable attention because of the area's high seismic activity. The largest recorded earthquakes in this area occurred in the winter of 1811-1812. These earthquakes has estimated magnitudes as large as 8.0 on the Richter scale (Johnsonton and Kanter, 1990) and affected an area of about 1 million square miles (Fuller, 1912). Today, an area of continuously high seismic activity defines the New Madrid seismic zone, which extends from northeastern Arkansas into southeastern Missouri and northwestern Tennessee. Seismicity is locally concentrated along two subsurface archers--the Blytheville and Pascola (Hildenbrand and others, 1977; Crone and others, 1985; Hildenbrand, 1985; McKeown, 1988). The Padcola arch is not pertinent to this study and, therefore will not be discusses further. The Blytheville arch is located in and is subparallel to the axis of the northeast-southwest-trending Reelfoot structural basin, which formed during early Paleozoic rifting (Ervin and McGinnis, 1975; fig. 1). The Reelfoot basin is filled with Cambrian and Ordovician sedimentary rocks (Grohskopf, 1955; Howe, 1984; Houseknevht, 1989; Collins and others, 1992) that are uncomfortably overlain by Cretacaous and Tertiary sedimentary rocks and underlain by crystalline rocks of the eastern granite-rhyolite province (see Bickford and others, 1986). The presence of some Late Proterozoic sedimentary rocks in the Reelfoot basin currently cannot be ruled out. The Dow Chemical #1 B.L. Garrigan drill hole (hereafter, Garrigan) penetrated Paleozoic rocks on the Blytheville arch. The Garrigan is locted in the Reelfoot basin in the NW1/4, NW1/4 sec. 28, T. 15 N., R. 10 E., Mississippi County, Arkansas (fig. 1) and was completed to a total depth of 12,038 ft from a ground elevation of 239 ft on April 11, 1982 (Swolfs, 1991). The Garrigan is the only reported drill hole that penetrates the subsurface Blytheville

  3. Characterization of the enterovirus 71 VP1 protein as a vaccine candidate.

    Science.gov (United States)

    Zhou, Shi-Li; Ying, Xiao-Ling; Han, Xue; Sun, Xian-Xun; Jin, Qi; Yang, Fan

    2015-02-01

    Enterovirus 71 (EV71) is an important agent responsible for hand-foot-and-mouth disease (HFMD), which can cause severe neurological complications and death in children. However, there is no specific treatment for EV71 infection, and a safe and effective vaccine is needed urgently. In this study, an effective and economical method for the production of EV71-VP1 protein was developed, and the VP1 protein was evaluated in humoral and cellular immune responses as an EV71 vaccine. The results revealed that the VP1 protein induced high titers of cross-neutralizing antibodies for different EV71 subtypes, and elicited significant splenocyte proliferation. The high levels of IFN-r and IL-10 showed the VP1 protein induced a mixed Th1 and Th2 immune response. Vaccinated female mice could confer protection in their neonatal offspring. Compared with the inactivated EV71, the VP1 protein elicited similar humoral and cellular responses, but the engineered protein is safer, less expensive and can be produced more efficiently. Therefore, EV71-VP1 protein can induce effective immunologic protection against EV71 and is an ideal candidate against EV71 infection. PMID:25043151

  4. Human IgG subclasses against enterovirus Type 71: neutralization versus antibody dependent enhancement of infection.

    Directory of Open Access Journals (Sweden)

    Rui-Yuan Cao

    Full Text Available The emerging human enterovirus 71 (EV71 represents a growing threat to public health, and no vaccine or specific antiviral is currently available. Human intravenous immunoglobulin (IVIG is clinical used in treating severe EV71 infections. However, the discovery of antibody dependent enhancement (ADE of EV71 infection illustrates the complex roles of antibody in controlling EV71 infection. In this study, to identify the distinct role of each IgG subclass on neutralization and enhancement of EV71 infection, different lots of pharmaceutical IVIG preparations manufactured from Chinese donors were used for IgG subclass fractionation by pH gradient elution with the protein A-conjugated affinity column. The neutralization and ADE capacities on EV71 infection of each purified IgG subclass were then assayed, respectively. The neutralizing activity of human IVIG is mainly mediated by IgG1 subclass and to less extent by IgG2 subclass. Interestingly, IgG3 fraction did not have neutralizing activity but enhanced EV71 infection in vitro. These results revealed the different roles of human IgG subclasses on EV71 infection, which is of critical importance for the rational design of immunotherapy and vaccines against severe EV71 diseases.

  5. High sensitivity and label-free detection of Enterovirus 71 by nanogold modified electrochemical impedance spectroscopy

    Science.gov (United States)

    Wang, Fang-Yu; Li, Hsing-Yuan; Tseng, Shing-Hua; Cheng, Tsai-Mu; Chu, Hsueh-Liang; Yang, Jyh-Yuan; Chang, Chia-Ching

    2013-03-01

    Enterovirus 71 (EV71), which is the most fulminant and invasive species of enterovirus, can cause children neurologic complications and death within 2-3 days after fever and rash developed. Besides, EV71 has high sequence similarity with Coxsackie A 16 (CA16) that makes differential diagnosis difficult in clinic and laboratory. Since conventional viral diagnostic method cannot diagnose EV71 quickly and EV71 can transmit at low viral titer, the patients might delay in treatment. A quick, high sensitive, and high specific test for EV71 detection is pivotal. Electrochemical impedance spectroscopy (EIS) has been applied for detecting bio-molecules as biosensors recently. In this study, we try to build a detection platform for EV71 detection by nanogold modified EIS probe. The result shows that our probe can detect 3.6 VP1/50 μl (one EV71 particle has 60 VP1) in 3 minutes. The test can also distinguish EV71 from CA16 and lysozyme. Diagnosis of enterovirus 71 by electrochemical impedance spectroscopy has the potential to apply in clinic.

  6. Association of Enterovirus 71 encephalitis with the interleukin-8 gene region in Chinese children.

    Science.gov (United States)

    Li, Jian; Lin, Aiwei; Yu, Chengwen; Zhang, Zhaofang; Xu, Daoyan; Hu, Wei; Liu, Liyan; Wang, Shaoning; Nie, Xiuzhen; Sun, Wenhui; Gai, Zhongtao; Chen, Zongbo

    2015-06-01

    The study was performed in 36 Chinese patients with Enterovirus 71 (EV71) encephalitis and 141 patients with EV71-related hand, foot and mouth disease (HFMD) without encephalitis. Genotyping was determined by polymerase chain reaction- restriction fragment length polymorphism. Patients with EV71 encephalitis had a significantly higher frequency of interleukin-8 (IL-8)-251TT genotype than patients with EV71-related HFMD without encephalitis (55.6% vs 31.2%, p = 0.023). The frequency of IL-8-251T alleles was significantly higher among patients with EV71 encephalitis than in patients with EV71-related HFMD without encephalitis (72.2% vs 58.9%, odds ratio 1.8, 95% confidence interval 1.0-3.2, p = 0.038). There were significant differences in gender, age, fever days, white blood cell count, C-reactive protein and blood glucose concentration and IL-8 levels among genotypes of IL-8-251A/T in EV71-infected patients, but no significant differences in alanine or aspartate aminotransferase, creatine kinase-myocardial isozyme and cerebrospinal fluid in patients with EV71 encephalitis. These findings suggest that the IL-8-251T allele is associated with susceptibility to EV71 encephalitis in Chinese patients. PMID:25751776

  7. Enterovirus 71 inhibits cellular type I interferon signaling by downregulating JAK1 protein expression.

    Science.gov (United States)

    Liu, Ying; Zhang, Zhe; Zhao, Xinghui; Yu, Rui; Zhang, Xiaopeng; Wu, Shipo; Liu, Ju; Chi, Xiangyang; Song, Xiaohong; Fu, Ling; Yu, Yingqun; Hou, Lihua; Chen, Wei

    2014-08-01

    Enterovirus 71 (EV71) infection can cause severe disease and lead to death in children. Recurring outbreaks of EV71 have been reported in several countries. Interferons (IFNs) have been used for decades to treat several types of viral infection, but have a limited ability to inhibit EV71 replication. Herein, we intend to investigate the mechanisms by which EV71 inhibits the cellular type I IFN response. In this study, MRC-5 (human embryonic lung fibroblast) or RD (human rhabdomyosarcoma) cells were infected with EV71, and then treated with or without IFN-α2b. Cells were harvested and analyzed by flow cytometry to determine the level of IFNAR1. Cell lysis were prepared to detect the levels of STAT1, STAT2, phosphorylated STAT1, phosphorylated STAT2, IFNAR1, JAK1, and TYK2 by Western blotting. The phosphorylation of STAT1 and STAT2 induced by IFN were inhibited without significant downregulation of IFNAR1 in EV71-infected cells. The EV71-induced suppression of STAT1 and STAT2 phosphorylation was not rescued by the protein tyrosine phosphatases inhibitor, and was independent of suppressor of cytokine signaling protein 1/3 levels. The phosphorylation of JAK1 and TYK2 were inhibited accompanied by EV71-induced downregulation of JAK1, which occurred at a post-transcriptional level and was proteasome independent. JAK1 expression did not decrease, and IFN-α-stimulated STAT1 and STAT2 phosphorylation were not blocked in HEK293T cells overexpressing the EV71 viral protein 2A or 3C. This study demonstrates that EV71 inhibits the cellular type I IFN antiviral pathway by downregulating JAK1, while the expression of IFNAR1 does not significantly alter in EV71-infected cells. Additionally, the EV71 viral proteins 2A and 3C do not act as antagonists of cellular type I IFN signaling. PMID:24905060

  8. Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection.

    Directory of Open Access Journals (Sweden)

    Yorihiro Nishimura

    Full Text Available Enterovirus 71 (EV71 is one of the major causative agents of hand, foot, and mouth disease, a common febrile disease in children; however, EV71 has been also associated with various neurological diseases including fatal cases in large EV71 outbreaks particularly in the Asia Pacific region. Recently we identified human P-selectin glycoprotein ligand-1 (PSGL-1 as a cellular receptor for entry and replication of EV71 in leukocytes. PSGL-1 is a sialomucin expressed on the surface of leukocytes, serves as a high affinity counterreceptor for selectins, and mediates leukocyte rolling on the endothelium. The PSGL-1-P-selectin interaction requires sulfation of at least one of three clustered tyrosines and an adjacent O-glycan expressing sialyl Lewis x in an N-terminal region of PSGL-1. To elucidate the molecular basis of the PSGL-1-EV71 interaction, we generated a series of PSGL-1 mutants and identified the post-translational modifications that are critical for binding of PSGL-1 to EV71. We expressed the PSGL-1 mutants in 293T cells and the transfected cells were assayed for their abilities to bind to EV71 by flow cytometry. We found that O-glycosylation on T57, which is critical for PSGL-1-selectin interaction, is not necessary for PSGL-1 binding to EV71. On the other hand, site-directed mutagenesis at one or more potential tyrosine sulfation sites in the N-terminal region of PSGL-1 significantly impaired PSGL-1 binding to EV71. Furthermore, an inhibitor of sulfation, sodium chlorate, blocked the PSGL-1-EV71 interaction and inhibited PSGL-1-mediated viral replication of EV71 in Jurkat T cells in a dose-dependent manner. Thus, the results presented in this study reveal that tyrosine sulfation, but not O-glycosylation, in the N-terminal region of PSGL-1 may facilitate virus entry and replication of EV71 in leukocytes.

  9. Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.

    Science.gov (United States)

    Ye, Xiaohua; Fan, Chen; Ku, Zhiqiang; Zuo, Teng; Kong, Liangliang; Zhang, Chao; Shi, Jinping; Liu, Qingwei; Chen, Tan; Zhang, Yingyi; Jiang, Wen; Zhang, Linqi; Huang, Zhong; Cong, Yao

    2016-03-01

    Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3) of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2), the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection. PMID:26938634

  10. Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.

    Directory of Open Access Journals (Sweden)

    Xiaohua Ye

    2016-03-01

    Full Text Available Enterovirus 71 (EV71 is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3 of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2, the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection.

  11. Penning-trap Q-value determination of the 71Ga(ν,e−)71Ge reaction using threshold charge breeding of on-line produced isotopes

    International Nuclear Information System (INIS)

    We present a first direct Q-value measurement of the 71Ga(ν,e−)71Ge reaction using the TITAN mass-measurement facility at ISAC/TRIUMF. The measurements were performed in a Penning trap on neon-like 71Ga21+ and 71Ge22+ using isobar separation of the on-line produced mother and daughter nuclei through threshold charge breeding in an electron-beam ion trap. In addition, isoionic samples of 71Ga21+ and 71Ge21+ were stored concurrently in the Penning trap and provided a separate Q-value measurement. Both independent measurements result in a combined Q-value of 233.5±1.2 keV, which is in agreement with the previously accepted Q-value for the ν cross-section calculations. Together with a recent measurement of the ν-response from the excited states in 71Ge, we conclude that there are no further uncertainties in the nuclear structure, which could remove the persistent discrepancy between the SAGE and GALLEX calibration measurements performed with neutrinos from reactor-produced 51Cr and 37Ar sources and the theoretical expectation

  12. 肠道病毒71型的分子流行病学现况%Molecular Epidemiological Research on Enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    檀晓娟; 许文波

    2008-01-01

    目的 肠道病毒71(Enterovirus 71,EV71)是导致婴幼儿感染的重要病原之一,可引起多种疾病,具有较广的疾病谱.EV71可引起大规模的手足口病(Hand,Foot,and Mouth Disease,HFMD)爆发,也可引起脑干脑炎等神经系统感染,导致死亡.现对EV71分子流行病学研究方法和研究进展进行综述,总结和分析了EV71各种基因型/亚型毒株的进化史及进化地位,对国际上EV71各种基因型/亚型的流行现况进行了概括总结.

  13. 肠道病毒71型疫苗研究进展%Research Progress of the Enterovirus Type 71 Vaccine

    Institute of Scientific and Technical Information of China (English)

    李燕; 刘燕敏; 梁晓峰

    2011-01-01

    近几年来,手足口病(Hand,Foot and Mouth Disease,HFMD)受到越来越多的关注.肠道病毒71型(Enterovirus Type 71,EV71)是引起HFMD的病原体之一.由于HFMD重症或死亡病例以EV71为主要病原,且该病尚无特效的治疗药物,因此EV71疫苗成为预防HFMD有效的措施.国内外已有灭活疫苗、减毒活疫苗、亚单位疫苗、病毒样颗粒和脱氧核糖核酸疫苗等多种尝试,但这些疫苗仅在动物模型中进行了评价,尚未实施临床试验.现将EV71疫苗研究进展进行综述.%Recent years, hand, foot and mouth disease(HFMD)caused more and more attention. Enterovirus type 71 (EV71)is one of pathogen caused HFMD. EV7I vaccine is an effective measure to prevent HFMD because of most severe cases and deaths caused by EV7, and there is no effective drugs so far. There are several vaccine candidates! Including inactivated whole virus vaccine?live-attenuated virus vaccine, subunit vaccine, virus-like particle and deoxyribonucleic acid vaccines, which have been trial and evaluated in animals. Clinical trial, however, has not been conducted. The research progress of EV71 vaccine was reviewed in this paper.

  14. Search for neutrinos from the Sun using the reaction sup 71 Ga(. nu. sub e , e sup minus ) sup 71 Ge

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, A.I.; Anosov, O.L.; Faizov, E.L.; Gavrin, V.N.; Kalikhov, A.V.; Knodel, T.V.; Knyshenko, I.I.; Kornoukhov, V.N.; Mezentseva, S.A.; Mirmov, I.N.; Ostrinsky, A.V.; Pshukov, A.M.; Revzin, N.E.; Shikhin, A.A.; Timofeyev, P.V.; Veretenkin, E.P.; Vermul, V.M.; Zatsepin, G.T. (Institute for Nuclear Research, Academy of Sciences, U.S.S.R., Moscow 117312 (USSR)); Bowles, T.J.; Cleveland, B.T.; Elliott, S.R.; O' Brien, H.A.; Wark, D.L.; Wilkerson, J.F. (Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)); Davis, R. Jr.; Lande, K. (University of Pennsylvania, Philadelphia, Pennsylvania 19104 (United States)); Cherry, M.L. (Louisiana State University, Baton Rouge, Louisiana 70803 (United States)); Kouzes, R.T. (Princeton University, Princeton, New Jersey 08544 (United States))

    1991-12-09

    A radiochemical {sup 71}Ga-{sup 71}Ge experiment using 30 tons of gallium to determine the primary flux of neutrinos from the Sun has begun operation at the Baksan Neutrino Observatory in the U.S.S.R. Assuming that the extraction efficiency for {sup 71}Ge atoms produced by solar neutrinos is the same as from natural Ge carrier, we observed the capture rate to be 20{sub {minus}20}{sup +15}(stat){plus minus}32(syst) solar neutrino units (SNU), resulting in a limit of less than 79 SNU (90% C.L.). This is to be compared with 132 SNU predicted by the standard solar model.

  15. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wenjing; Tao, Junyan; Yang, Xiaoping [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China); Yang, Zhuliang [Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201 (China); Zhang, Li; Liu, Hongsheng [Department of Academy of Sciences, Liaoning University, Shenyang 110036 (China); Wu, Kailang [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China); Wu, Jianguo, E-mail: jwu@whu.edu.cn [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China)

    2014-07-04

    Highlights: • Triterpenoids GLTA and GLTB display anti-EV71 activities without cytotoxicity. • The compounds prevent EV71 infection by blocking adsorption of the virus to the cells. • GLTA and GLTB bind to EV71 capsid at the hydrophobic pocket to block EV71 uncoating. • The two compounds significantly inhibit the replication of EV71 viral RNA. • GLTA and GLTB may be used as potential therapeutic agents to treat EV71 infection. - Abstract: Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential

  16. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection

    International Nuclear Information System (INIS)

    Highlights: • Triterpenoids GLTA and GLTB display anti-EV71 activities without cytotoxicity. • The compounds prevent EV71 infection by blocking adsorption of the virus to the cells. • GLTA and GLTB bind to EV71 capsid at the hydrophobic pocket to block EV71 uncoating. • The two compounds significantly inhibit the replication of EV71 viral RNA. • GLTA and GLTB may be used as potential therapeutic agents to treat EV71 infection. - Abstract: Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential

  17. 71st Colloquium of the International Astronomical Union

    CERN Document Server

    Rodonò, Marcello

    1983-01-01

    IAU Colloquium No. 71 had its immediate origins in a small gathering of people interested. in the optical and UV study of flare stars which took place during the 1979 Montreal General Assembly. We recognized that a fundamental change was taking place in the study of these objects. Space-borne instruments (especially lUE and Einstein) and a new genera­ tion of ground-based equipment were having a profound effect on the range of investigations it was possible to make. To extract maximum benefit from these new possibilities it would be necessary as never before to have good communication with colleagues in other disciplines, for instance,. with atomic and solar physicists. Similarly, studies of phenomena associated with the outer atmospheres of the late-type stars could now hope to give significant insights into certain aspects of solar activity. So, in view of the wide range of backgrounds of those participating, the meeting had an unusually high proportion of invited reviews while most of the contributed pape...

  18. PD-L1 interacts specifically with B7-1 to inhibit T cell proliferation

    OpenAIRE

    Butte, Manish J.; Keir, Mary E.; Phamduy, Theresa B.; Freeman, Gordon J; Sharpe, Arlene H.

    2007-01-01

    Pathways in the B7:CD28 family regulate T cell activation and tolerance. B7 dependent responses in CD28/CTLA-4-/- T cells together with reports of stimulatory and inhibitory functions for PD-L1 and PD-L2 have suggested additional receptors for these B7 family members. We show that B7-1 and PD-L1 interact with an affinity intermediate to that of B7-1:CD28 and B7-1:CTLA-4. The PD-L1:B7-1 interface overlaps with the B7-1:CTLA-4 and PD-L1:PD-1 interfaces. We show that the interaction of B7-1 with...

  19. Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix

    International Nuclear Information System (INIS)

    The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in Center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation

  20. Risk Factors for Enterovirus A71 Seropositivity in Rural Indigenous Populations in West Malaysia

    Science.gov (United States)

    NikNadia, NMN; Sam, I-Ching; Khaidir, Nasibah; Ngui, Romano; Lim, Yvonne A. L.; Goh, Xiang Ting; Choy, Seow Huey; Chan, Yoke Fun

    2016-01-01

    Enterovirus A71 (EV-A71), which is transmitted by the fecal-oral route, causes hand, foot and mouth disease and, rarely, severe neurological complications. In Malaysia, the indigenous rural community (Orang Asli) has a high prevalence of parasitic diseases due to poor sanitation, water supply and hygiene practices. This cross-sectional study compared the seroepidemiology of EV-A71 among rural Orang Asli and urban Kuala Lumpur populations in West Malaysia, and determined the risk factors associated with EV-A71 seropositivity in rural Orang Asli. Seropositive rates were determined by neutralization assay. EV-A71 seropositivity was strongly associated with increasing age in both populations. Rural Orang Asli children ≤12 years had significantly higher EV-A71 seropositivity rates than urban Kuala Lumpur children (95.5% vs 57.6%, P vaccination programs in Malaysia. PMID:26866912

  1. Design, synthesis, and biological evaluation of anti-EV71 agents.

    Science.gov (United States)

    Li, Peng; Yang, Bailing; Hao, Fei; Wang, Ping; He, Haiying; Huang, Lei; Zhang, Xuan; Zhang, Shengbin; Peng, Xuanjia; Yin, Ke; Hu, Jiao; Chen, Xinsheng; Gu, Zhengxian; Wang, Li; Shen, Liang; Hu, Guoping; Li, Ning; Li, Jian; Chen, Shuhui; Xiao, Wei; Wang, Zhenzhong; Guo, Qingming; Chang, Xiujuan; Zhang, Lanjun; Cai, Qixu; Lin, Tianwei

    2016-07-15

    Enterovirus 71 (EV71) is a major causative agent of hand, foot and mouth disease (HFMD), which can spread its infections to the central nervous and other systems with severe consequences. In this article, design, chemical synthesis, and biological evaluation of various anti-EV71 agents which incorporate Michael acceptors are described. Further SAR study demonstrated that lactone type of Michael acceptor provided a new lead of anti-EV71 drug candidates with high anti-EV71 activity in cell-based assay and enhanced mouse plasma stability. One of the most potent compounds (2K, cell-based anti-EV71 EC50=0.028μM), showed acceptable stability profile towards mouse plasma, which resulted into promising pharmacokinetics in mouse via IP administration. PMID:27234148

  2. Lycorine reduces mortality of human enterovirus 71-infected mice by inhibiting virus replication

    Directory of Open Access Journals (Sweden)

    Qin Chuan

    2011-10-01

    Full Text Available Abstract Human enterovirus 71 (EV71 infection causes hand, foot and mouth disease in children under 6 years old and this infection occasionally induces severe neurological complications. No vaccines or drugs are clinical available to control EV71 epidemics. In present study, we show that treatment with lycorine reduced the viral cytopathic effect (CPE on rhabdomyosarcoma (RD cells by inhibiting virus replication. Analysis of this inhibitory effect of lycorine on viral proteins synthesis suggests that lycorine blocks the elongation of the viral polyprotein during translation. Lycorine treatment of mice challenged with a lethal dose of EV71 resulted in reduction of mortality, clinical scores and pathological changes in the muscles of mice, which were achieved through inhibition of viral replication. When mice were infected with a moderate dose of EV71, lycorine treatment was able to protect them from paralysis. Lycorine may be a potential drug candidate for the clinical treatment of EV71-infected patients.

  3. Risk Factors for Enterovirus A71 Seropositivity in Rural Indigenous Populations in West Malaysia.

    Science.gov (United States)

    NikNadia, Nmn; Sam, I-Ching; Khaidir, Nasibah; Ngui, Romano; Lim, Yvonne A L; Goh, Xiang Ting; Choy, Seow Huey; Chan, Yoke Fun

    2016-01-01

    Enterovirus A71 (EV-A71), which is transmitted by the fecal-oral route, causes hand, foot and mouth disease and, rarely, severe neurological complications. In Malaysia, the indigenous rural community (Orang Asli) has a high prevalence of parasitic diseases due to poor sanitation, water supply and hygiene practices. This cross-sectional study compared the seroepidemiology of EV-A71 among rural Orang Asli and urban Kuala Lumpur populations in West Malaysia, and determined the risk factors associated with EV-A71 seropositivity in rural Orang Asli. Seropositive rates were determined by neutralization assay. EV-A71 seropositivity was strongly associated with increasing age in both populations. Rural Orang Asli children ≤12 years had significantly higher EV-A71 seropositivity rates than urban Kuala Lumpur children (95.5% vs 57.6%, P children should be a priority target for future vaccination programs in Malaysia. PMID:26866912

  4. Risk Factors for Enterovirus A71 Seropositivity in Rural Indigenous Populations in West Malaysia

    OpenAIRE

    NikNadia, NMN; Sam, I-Ching; Khaidir, Nasibah; Ngui, Romano; Lim, Yvonne A. L.; Goh, Xiang Ting; Choy, Seow Huey; Chan, Yoke Fun

    2016-01-01

    Enterovirus A71 (EV-A71), which is transmitted by the fecal-oral route, causes hand, foot and mouth disease and, rarely, severe neurological complications. In Malaysia, the indigenous rural community (Orang Asli) has a high prevalence of parasitic diseases due to poor sanitation, water supply and hygiene practices. This cross-sectional study compared the seroepidemiology of EV-A71 among rural Orang Asli and urban Kuala Lumpur populations in West Malaysia, and determined the risk factors assoc...

  5. The Enterovirus 71 Procapsid Binds Neutralizing Antibodies and Rescues Virus Infection In Vitro

    OpenAIRE

    Shingler, Kristin L.; Cifuente, Javier O.; Ashley, Robert E.; Makhov, Alexander M.; Conway, James F; Hafenstein, Susan

    2014-01-01

    Enterovirus 71 (EV71) is responsible for seasonal outbreaks of hand, foot, and mouth disease in the Asia-Pacific region. The virus has the capability to cause severe disease and death, especially in young children. Although several vaccines are currently in clinical trials, no vaccines or therapeutics have been approved for use. Previous structural studies have revealed that two antigenically distinct capsid forms are produced in EV71-infected cells: an expanded empty capsid, sometimes called...

  6. 26 CFR 1.501(c)(7)-1 - Social clubs.

    Science.gov (United States)

    2010-04-01

    ... activities. (b) A club which engages in business, such as making its social and recreational facilities... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Social clubs. 1.501(c)(7)-1 Section 1.501(c)(7)-1...) INCOME TAXES (CONTINUED) Exempt Organizations § 1.501(c)(7)-1 Social clubs. (a) The exemption provided...

  7. A Colorimetric-Based Accurate Method for the Determination of Enterovirus 71 Titer

    OpenAIRE

    Pourianfar, Hamid Reza; Javadi, Arman; Grollo, Lara

    2012-01-01

    The 50 % tissue culture infectious dose (TCID50) is still one of the most commonly used techniques for estimating virus titers. However, the traditional TCID50 assay is time consuming, susceptible to subjective errors and generates only quantal data. Here, we describe a colorimetric-based approach for the titration of Enterovirus 71 (EV71) using a modified method for making virus dilutions. In summary, the titration of EV71 using MTT or MTS staining with a modified virus dilution method decre...

  8. Class I ADP-Ribosylation Factors Are Involved in Enterovirus 71 Replication

    OpenAIRE

    Wang, Jianmin; Du, Jiang; Jin, Qi

    2014-01-01

    Enterovirus 71 is one of the major causative agents of hand, foot, and mouth disease in infants and children. Replication of enterovirus 71 depends on host cellular factors. The viral replication complex is formed in novel, cytoplasmic, vesicular compartments. It has not been elucidated which cellular pathways are hijacked by the virus to create these vesicles. Here, we investigated whether proteins associated with the cellular secretory pathway were involved in enterovirus 71 replication. We...

  9. Deferoxamine Compensates for Decreases in B Cell Counts and Reduces Mortality in Enterovirus 71-Infected Mice

    OpenAIRE

    Yajun Yang; Jing Ma; Jinghui Xiu; Lin Bai; Feifei Guan; Li Zhang; Jiangning Liu; Lianfeng Zhang

    2014-01-01

    Enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. No vaccine or antiviral therapy is currently available. In this work, we found that the number of B cells was reduced in enterovirus 71-infected mice. Deferoxamine, a marine microbial natural product, compensated for the decreased levels of B cells caused by enterovirus 71 infection. The neutralizing antibody titer was also improved after deferoxamine treatment. Furthermore...

  10. Enterovirus A71 Meningoencephalitis Outbreak, Rostov-on-Don, Russia, 2013

    OpenAIRE

    Akhmadishina, Ludmila V.; Govorukhina, Marina V.; Kovalev, Evgeniy V.; Nenadskaya, Svetlana A.; Ivanova, Olga E.; Lukashev, Alexander N.

    2015-01-01

    Seventy-eight cases of enterovirus infection, including 25 neuroinfections, occurred in Rostov-on-Don, Russia, during May–June 2013. The outbreak was caused by an enterovirus A type 71 (EV-A71) subgenotype C4 lineage that spread to neighboring countries from China ≈3 years earlier. Enterovirus associated neuroinfection may emerge in areas with a preceding background circulation of EV-A71 with apparently asymptomatic infection.

  11. Full-Genome Sequences of Seven Fatal Enterovirus 71 Strains Isolated in Shenzhen, China, in 2014.

    Science.gov (United States)

    Chen, Long; He, Ya-Qing; Meng, Jun; Xiong, Ling-Hong; Wang, Chao; Yao, Xiang-Jie; Zhang, Hai-Long; Zhang, Ren-Li; Yang, Hong

    2016-01-01

    The whole-genome sequences of seven fatal enterovirus 71 (EV71) strains, isolated in southern China, in 2014, were determined. The complete genome sequences of these strains displayed close relationships to native EV71 strains and showed 94.2% to 99.8% identity to each other. All of these strains were assigned to subgenotype C4a based on phylogenetic analysis of the VP1 gene. PMID:27125487

  12. Enterovirus 71 virion-associated galectin-1 facilitates viral replication and stability.

    Directory of Open Access Journals (Sweden)

    Pei-Huan Lee

    Full Text Available Enterovirus 71 (EV71 infection causes a myriad of diseases from mild hand-foot-and-mouth disease or herpangina to fatal brain stem encephalitis complicated with pulmonary edema. Several severe EV71 endemics have occurred in Asia-Pacific region, including Taiwan, and have become a serious threat to children's health. EV71 infection is initiated by the attachment of the virion to the target cell surface. Although this process relies primarily upon interaction between viruses and cell surface receptors, soluble factors may also influence the binding of EV71 to host cells. Galectin-1 has been reported to participate in several virus infections, but is not addressed in EV71. In this study, we found that the serum levels of galectin-1 in EV71-infected children were higher than those in non-infected people. In EV71 infected cells, galectin-1 was found to be associated with the EV71 VP1 and VP3 via carbohydrate residues and subsequently released and bound to another cell surface along with the virus. EV71 propagated from galectin-1 knockdown SK-N-SH cells exhibited lower infectivity in cultured cells and less pathogenicity in mice than the virus propagated from parental cells. In addition, this galectin-1-free EV71 virus was sensitive to high temperature and lost its viability after long-term storage, which could be restored following supplement of recombinant galectin-1. Taken together, our findings uncover a new role of galectin-1 in facilitating EV71 virus infection.

  13. 肠道病毒71型的研究进展

    Institute of Scientific and Technical Information of China (English)

    陈凡; 齐顺祥; 王永祥

    2009-01-01

    @@ 肠道病毒71型(enterovirus 71,EV71)感染主要引起手足口病(hand foot and mouth disease,HFMD).此外,还能引起无菌性脑膜炎、脑干脑炎和脊髓灰质炎样麻痹等多种神经系统疾病[1].

  14. Public-health assessment for Monroe Auto Equipment Company (Paragould Pit), Paragould, Greene County, Arkansas, Region 6. CERCLIS No. ARD980864110. Preliminary report

    Energy Technology Data Exchange (ETDEWEB)

    1992-06-22

    The Monroe Auto Equipment Company site (aliases Paragould Pit and Finch Road Landfill) is an industrial landfill located near Paragould, Arkansas. The landfill received wastewater treatment sludge, which contained trichlorethylene and various heavy metals, from the Monroe Auto Equipment Company's Paragould manufacturing facility. Groundwater on-site is contaminated with volatile organic compounds and metals with concentrations of trans-1,2-dichloroethene, chromium and lead exceeding U.S. Environmental Protection Agency (EPA) drinking water standards. One off-site monitoring well has been shown to be contaminated with cadmium, chromium, and lead. Approximately 3 dozen households are within a 1-mile radius of the site. The entire area depends on groundwater for drinking water and domestic use. Subsurface soil is contaminated with chromium and lead. Exposure pathways of potential concern include ingestion and inhalation of and dermal contact with disturbed contaminated soil and volatilized organic compounds.

  15. Hydrologic and Water-Quality Characteristics for Calf Creek Near Silber Hill, Arkansas and Selected Buffalo River Sites, 2001-2002

    Science.gov (United States)

    Galloway, Joel M.; Green, W. Reed

    2004-01-01

    The Buffalo River and its tributary, Calf Creek, are in the White River Basin in the Ozark Plateaus physiographic province in north-central Arkansas. A better understanding of the hydrology and water quality of Calf Creek is of interest to many, including the National Park Service, which administers the Buffalo National River, to evaluate its effect on the hydrology and water quality of the Buffalo River. The streamflow and water-quality characteristics of Calf Creek near Silver Hill, Arkansas, were compared to two sites on the Buffalo River upstream (near Boxley, Arkansas) and downstream (near St. Joe, Arkansas) from the confluence of Calf Creek for calendar years 2001 and 2002. Annual and seasonal loads were estimated for Calf Creek for nutrients, dissolved organic carbon, and suspended sediment and compared with loads at sites on the Buffalo River. Flow-weighted concentrations and yields were computed from estimated annual loads for comparison with other developed and undeveloped basins. Streamflow varied annually and seasonally at the three sites. The Buffalo River near St. Joe had the largest annual mean streamflow (805 to 1,360 cubic feet per second for 2001 and 2002) compared to the Buffalo River near Boxley (106 and 152 cubic feet per second for 2001 and 2002) and Calf Creek (39 and 80 cubic feet per second for 2001 and 2002). Concentrations of nutrients, suspended sediment, and fecal indicator bacteria generally were greater in samples from Calf Creek than in samples collected from both Buffalo River sites. Bacteria and suspended-sediment concentrations were greater in samples collected during high-flow events at all three sites. The Buffalo River near Boxley had the lowest concentrations for nutrients, suspended sediment, and fecal indicator bacteria. Estimated annual loads of the nutrients, suspended sediment, and organic carbon for 2001 and 2002 demonstrated substantial variability between the three sites and through time. Estimated loads for nutrients

  16. Use of finite-difference arrays of observation wells to estimate evapotranspiration from ground water in the Arkansas River Valley, Colorado

    Science.gov (United States)

    Weeks, Edwin P.; Sorey, M.L.

    1973-01-01

    A method to determine evapotranspiration from ground water was tested at four sites in the flood plain of the Arkansas River in Colorado. Approximate ground-water budgets were obtained by analyzing water-level data from observation wells installed in five-point arrays. The analyses were based on finite difference approximations of the differential equation describing ground-water flow. Data from the sites were divided into two groups by season. It was assumed that water levels during the dormant season were unaffected by evapotranspiration of ground water or by recharge, collectively termed 'accretion.' Regression analyses of these data were made to provide an equation for separating the effects of changes in aquifer storage and of aquifer heterogeneity from those due to accretion during the growing season. The data collected during the growing season were thus analyzed to determine accretion.

  17. Cyclical Patterns of Hand, Foot and Mouth Disease Caused by Enterovirus A71 in Malaysia.

    OpenAIRE

    Nmn NikNadia; I-Ching Sam; Sanjay Rampal; Wmz WanNorAmalina; Ghazali NurAtifah; Khebir Verasahib; Chia Ching Ong; MohdAidinniza MohdAdib; Yoke Fun Chan

    2016-01-01

    Enterovirus A71 (EV-A71) is an important emerging pathogen causing large epidemics of hand, foot and mouth disease (HFMD) in children. In Malaysia, since the first EV-A71 epidemic in 1997, recurrent cyclical epidemics have occurred every 2–3 years for reasons that remain unclear. We hypothesize that this cyclical pattern is due to changes in population immunity in children (measured as seroprevalence). Neutralizing antibody titers against EV-A71 were measured in 2,141 residual serum samples c...

  18. Cyclical Patterns of Hand, Foot and Mouth Disease Caused by Enterovirus A71 in Malaysia.

    Science.gov (United States)

    NikNadia, Nmn; Sam, I-Ching; Rampal, Sanjay; WanNorAmalina, Wmz; NurAtifah, Ghazali; Verasahib, Khebir; Ong, Chia Ching; MohdAdib, MohdAidinniza; Chan, Yoke Fun

    2016-03-01

    Enterovirus A71 (EV-A71) is an important emerging pathogen causing large epidemics of hand, foot and mouth disease (HFMD) in children. In Malaysia, since the first EV-A71 epidemic in 1997, recurrent cyclical epidemics have occurred every 2-3 years for reasons that remain unclear. We hypothesize that this cyclical pattern is due to changes in population immunity in children (measured as seroprevalence). Neutralizing antibody titers against EV-A71 were measured in 2,141 residual serum samples collected from children ≤12 years old between 1995 and 2012 to determine the seroprevalence of EV-A71. Reported national HFMD incidence was highest in children children. EV-A71 epidemics are also characterized by peaks of increased genetic diversity, often with genotype changes. Cross-sectional time series analysis was used to model the association between EV-A71 epidemic periods and EV-A71 seroprevalence adjusting for age and climatic variables (temperature, rainfall, rain days and ultraviolet radiance). A 10% increase in absolute monthly EV-A71 seroprevalence was associated with a 45% higher odds of an epidemic (adjusted odds ratio, aOR1.45; 95% CI 1.24-1.69; PMalaysia is mainly due to the fall of population immunity accompanying the accumulation of susceptible children between epidemics. This study will impact the future planning, timing and target populations for vaccine programs. PMID:27010319

  19. Discovery of Gramine Derivatives That Inhibit the Early Stage of EV71 Replication in Vitro

    Directory of Open Access Journals (Sweden)

    Yanhong Wei

    2014-06-01

    Full Text Available Enterovirus 71 (EV71 is a notable causative agent of hand, foot, and mouth disease in children, which is associated with an increased incidence of severe neurological disease and death, yet there is no specific treatment or vaccine for EV71 infections. In this study, the antiviral activity of gramine and 21 gramine derivatives against EV71 was investigated in cell-based assays. Eighteen derivatives displayed some degree of inhibitory effects against EV71, in that they could effectively inhibit virus-induced cytopathic effects (CPEs, but the anti-EV71 activity of the lead compound gramine was not observed. Studies on the preliminary modes of action showed that these compounds functioned by targeting the early stage of the EV71 lifecycle after viral entry, rather than inactivating the virus directly, inhibiting virus adsorption or affecting viral release from the cells. Among these derivatives, one (compound 4s containing pyridine and benzothiazole units showed the most potency against EV71. Further studies demonstrated that derivative 4s could profoundly inhibit viral RNA replication, protein synthesis, and virus-induced apoptosis in RD cells. These results indicate that derivative 4s might be a feasible therapeutic agent against EV71 infection and that these gramine derivatives may provide promising lead scaffolds for the further design and synthesis of potential antiviral agents.

  20. Cyclical Patterns of Hand, Foot and Mouth Disease Caused by Enterovirus A71 in Malaysia

    OpenAIRE

    NikNadia, NMN; Sam, I-Ching; Rampal, Sanjay; WanNorAmalina, WMZ; NurAtifah, Ghazali; Verasahib, Khebir; Ong, Chia Ching; MohdAdib, MohdAidinniza; Chan, Yoke Fun

    2016-01-01

    Enterovirus A71 (EV-A71) is an important emerging pathogen causing large epidemics of hand, foot and mouth disease (HFMD) in children. In Malaysia, since the first EV-A71 epidemic in 1997, recurrent cyclical epidemics have occurred every 2–3 years for reasons that remain unclear. We hypothesize that this cyclical pattern is due to changes in population immunity in children (measured as seroprevalence). Neutralizing antibody titers against EV-A71 were measured in 2,141 residual serum samples c...