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Sample records for arkansas power-lsenic 71

  1. Slamming Arkansas Schools!

    Science.gov (United States)

    Scott, W. Clayton

    2010-01-01

    In this article, the author, a poet and teaching artist, shares how he successfully brought slam poetry to College Hill Middle School in Texarkana, Arkansas. In 2001 he discovered slam poetry--a poetry-reading format in which poets compete in dramatic readings of their works--and went to Slam Nationals in Seattle on the Arkansas slam team. He…

  2. Arkansas' Disappearing Tax Base.

    Science.gov (United States)

    Schoppmeyer, Martin; Venters, Tommy

    State legislation that has contributed to the reduction of Arkansas' tax base is described in this paper. Amendment 59, adopted in 1980, has reduced the state tax base by millions of dollars. At the end of 1992, the majority of school districts have equalized their real, personal, and carrier and utility property. Act 34, the current foundation…

  3. Arkansas River Water Needs Assessment

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report is on the legal elements, hydrologic analysis, objectives, and water levels related to the Arkansas River and the management of it.

  4. Marshallese COFA Migrants in Arkansas.

    Science.gov (United States)

    McElfish, Pearl Anna

    2016-05-01

    Arkansas is home to one of the largest populations of Marshallese in the world. Marshallese communities suffer from a disproportionate incidence of chronic diseases, including obesity, cardiovascular disease, diabetes, and infectious diseases, such as Hansen's disease (leprosy), tuberculosis, and types of hepatitis. There are a number of structural, legal, economic, and social issues that must be addressed in order to reduce health disparities and increase access to health care for Marshallese living in Arkansas. PMID:27263176

  5. 33 CFR 117.121 - Arkansas River

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Arkansas River 117.121 Section 117.121 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Arkansas § 117.121 Arkansas River The draw of...

  6. Abortion applicants in Arkansas.

    Science.gov (United States)

    Henker, F O

    1973-03-01

    The article reports upon the characteristics of 300 abortion applicants in Arkansas manifesting significant stress from unwanted pregnancy between May 1, 1970 and June 30, 1971. The sample is limited by the fact that all of these women had been willing to seek medical aid. Patients ranged from ages 13-47, 131 of them ages 17-21. 35% had had some college education; another 29% were high school graduates. 50.6%, 20.6%, and 27.3% were single, divorced, and married, respectively. 59.6% of the patients were primiparas. 18.3%, 9.6%, and 12.3% were classified as being neurotic, having psychophysiologic tendencies (gastrointestinal problems, obesity, chronic headaches), and having sociopathic features (passive-aggressive, frankly rebellious, delinquent, antisocial, alcoholic), respectively. 12 women had noticeable schizoid features; 4 women had mildly active schizophrenia. Fathers of the women were usually blue-collar workers (55.3%) or white-collar workers (24.6%). The most frequent ordinal sibling position among the women was oldest child (38%). Parental instability (1 or both parents lost through death, divorce, father usually away working, chronic alcoholism, etc.) was reported by 39.6% of the patients. Patients' attitudes toward the unwanted pregnancy included dislike of inexpediency of the situation (82.6%), self-depreciation (55.6%), and aversion (28.6%). Precipitated psychiatric disorders were for the greatest part mild. Manifesting symptoms included depression (66.7%), anxiety (21%), and mixed anxiety and depression (12.2%). Suicidal threats and gestures were made by 22 and 8 patients, respectively. In summary, the study reveals a group of predominantly Caucasian women from unstable, middle-class urban families who were going through an adjustment reaction to adolescence or adult life.

  7. Arkansas: Its Land and People, Vol. 1.

    Science.gov (United States)

    Foti, Tom

    This bicentennial volume offers a blend of Arkansas history, ecology, and literature. Its purpose is to show the relationship between Arkansas' people and their land. It contains a discussion of the Natural Divisions concept, the geological development of the state, and descriptions by early pioneers. The volume is not intended to be a complete…

  8. Arkansas State University Beebe Branch Faculty Handbook.

    Science.gov (United States)

    Arkansas State Univ., Beebe.

    Arkansas State University Beebe Branch provides a liberal arts oriented program for traditional and nontraditional students. Its faculty handbook contains institutional goals, description of responsibilities of administrative officers and faculty committees, faculty employment policies, and administrative and instructional policies. The…

  9. Arkansas' Children: How Well Are They Doing? Arkansas Kids Count Data Book 1996.

    Science.gov (United States)

    Dahlstrom, Sherryl M.; High, Rhonda L.

    This Kids Count data book is the fourth to examine the well-being of Arkansas' children and notes trends from 1990 to 1995. The report's introductory sections include discussions of the potential impact of welfare reform on Arkansas' children, and present figures detailing the number of children affected by particular risk factors each week. The…

  10. Arkansas' Junior Executive Training Curriculum Guide.

    Science.gov (United States)

    Clayton, Dean; And Others

    This curriculum guide is designed to help teachers conduct courses on small business management and entrepreneurship to high school seniors in Arkansas. The program focuses upon the managerial process, examining the functions of planning, organization, staffing, directing, and controlling as related to the activities and responsibilities of the…

  11. 78 FR 9448 - Arkansas Disaster #AR-00061

    Science.gov (United States)

    2013-02-08

    ... ADMINISTRATION Arkansas Disaster AR-00061 AGENCY: U.S. Small Business Administration. ACTION: Notice SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the... Disaster Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite 6050, Washington,...

  12. 27 CFR 9.112 - Arkansas Mountain.

    Science.gov (United States)

    2010-04-01

    ... Jean Creek. (vi) Then generally northeastward and eastward along Petit Jean Creek until it becomes the Petit Jean River (on the Russellville map). (vii) Then generally eastward along the Petit Jean River, flowing through Blue Mountain Lake, until the Petit Jean River joins the Arkansas River. (viii)...

  13. Rickettsiae in Gulf Coast Ticks, Arkansas, USA

    OpenAIRE

    Trout, Rebecca; Steelman, C Dayton; Szalanski, Allen L.; Williamson, Phillip C

    2010-01-01

    To determine the cause of spotted fever cases in the southern United States, we screened Gulf Coast ticks (Amblyomma maculatum) collected in Arkansas for rickettsiae. Of the screened ticks, 30% had PCR amplicons consistent with Rickettsia parkeri or Candidatus Rickettsia amblyommii.

  14. Identifying sites for elk restoration in Arkansas

    Science.gov (United States)

    Telesco, R.L.; Van Manen, F.T.; Clark, J.D.; Cartwright, Michael E.

    2007-01-01

    We used spatial data to identify potential areas for elk (Cervus elaphus) restoration in Arkansas. To assess habitat, we used locations of 239 elk groups collected from helicopter surveys in the Buffalo National River area of northwestern Arkansas, USA, from 1992 to 2002. We calculated the Mahalanobis distance (D2) statistic based on the relationship between those elk-group locations and a suite of 9 landscape variables to evaluate winter habitat in Arkansas. We tested model performance in the Buffalo National River area by comparing the D2 values of pixels representing areas with and without elk pellets along 19 fixed-width transects surveyed in March 2002. Pixels with elk scat had lower D2 values than pixels in which we found no pellets (logistic regression: Wald χ2 = 24.37, P cover, gently sloping ridge tops and valleys, low human population density, and low road densities. To assess the potential for elk–human conflicts in Arkansas, we used the analytical hierarchy process to rank the importance of 8 criteria based on expert opinion from biologists involved in elk management. The biologists ranked availability of forage on public lands as having the strongest influence on the potential for elk–human conflict (33%), followed by human population growth rate (22%) and the amount of private land in row crops (18%). We then applied those rankings in a weighted linear summation to map the relative potential for elk–human conflict. Finally, we used white-tailed deer (Odocoileus virginianus) densities to identify areas where success of elk restoration may be hampered due to meningeal worm (Parelaphostrongylus tenuis) transmission. By combining results of the 3 spatial data layers (i.e., habitat model, elk–human conflict model, deer density), our model indicated that restoration sites located in west-central and north-central Arkansas were most favorable for reintroduction.

  15. 78 FR 5202 - Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR

    Science.gov (United States)

    2013-01-24

    ... National Park Service Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Arkansas State University Museum... culturally affiliated with the human remains may contact the Arkansas State University Museum....

  16. 78 FR 5199 - Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR

    Science.gov (United States)

    2013-01-24

    ... National Park Service Notice of Inventory Completion: Arkansas State University Museum, Jonesboro, AR AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Arkansas State University Museum... associated funerary objects may contact the Arkansas State University Museum. Repatriation of the...

  17. Time of travel of selected Arkansas streams

    Science.gov (United States)

    Lamb, T.E.

    1982-01-01

    Between 1971 and 1981, time-of-travel and dispersion measurements were made in 15 streams in Arkansas. Most of the streams studied were at or near base flow. Graphs are presented for predicting traveltime of solutes in segments of the streams studied. The relationship of time of passage and peak unit concentration to traveltime is presented for two of the streams. Examples of use and application of the data are given. (USGS)

  18. Success For Arkansas: Success for All Schools In Arkansas Continue Gains On Benchmark Exams

    Science.gov (United States)

    Success for All Foundation, 2004

    2004-01-01

    Success for All is the most extensively researched of all comprehensive reform models for Title I elementary schools. It incorporates scientifically based principles of reading, cooperative learning, professional development, tutoring, and family support. Arkansas elementary schools using the Success for All reading program continued to make …

  19. A Look at Arkansas' Children: Arkansas Kids Count Data Book 1997.

    Science.gov (United States)

    Dahlstrom, Sherryl M.; High, Rhonda L.

    This Kids Count data book provides information on indicators of the well-being of Arkansas' children. The report's introduction discusses factors contributing to increased attention to children and families, the need for broad-based community collaborations to address children and family needs, and cultural changes influencing families. Data…

  20. 75 FR 44982 - Arkansas Valley Conduit (AVC) and Long-Term Excess Capacity Master Contract, Fryingpan-Arkansas...

    Science.gov (United States)

    2010-07-30

    ..., Fryingpan-Arkansas Project (Fry-Ark Project) Colorado AGENCY: Bureau of Reclamation, Interior. ACTION... proposed feature of the Fryingpan-Arkansas (Fry-Ark) Project, and the issuance of an Excess Capacity Master...-Ark Project water in Pueblo Reservoir, a feature of the Fry-Ark Project. The water would be used...

  1. Arkansas' Anemometer Loan Program

    Energy Technology Data Exchange (ETDEWEB)

    Fernando Vego

    2012-10-11

    The measurement campaign had one year duration from 04/01/2011 to 03/31/2012 and was taken at 20m and 34m with NRG instrumentation. The data was analyzed weekly to check inconsistencies and validity and processed using Excel, Flexpro and Windographer standard Edition Version 2.04. The site analyzed is located in the Waldron, Arkansas in Scott County. It is an open site for most of the direction sectors with immediate roughness class of 1.5. It has seasonally directional winds, of which the most energetic come from the southern direction. The vertical wind profile shows moderate wind shear that varies by season as well.

  2. 10 CFR 71.71 - Normal conditions of transport.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Normal conditions of transport. 71.71 Section 71.71 Energy..., Special Form, and LSA-III Tests 2 § 71.71 Normal conditions of transport. (a) Evaluation. Evaluation of each package design under normal conditions of transport must include a determination of the effect...

  3. Small Works in Arkansas: How Poverty and the Size of Schools and School Districts Affect School Performance in Arkansas. Summary of Recent Research.

    Science.gov (United States)

    Rural School and Community Trust, Washington, DC.

    A study examined how Arkansas students' achievement is related to poverty, school and district size, and the interaction between these factors. Achievement test scores from grades 4, 5, 7, 8, and 9 in all Arkansas schools were supplied by the Arkansas Department of Education. Poverty levels were determined by percentage of students receiving…

  4. Aquifers of Arkansas: protection, management, and hydrologic and geochemical characteristics of groundwater resources in Arkansas

    Science.gov (United States)

    Kresse, Timothy M.; Hays, Phillip D.; Merriman, Katherine R.; Gillip, Jonathan A.; Fugitt, D. Todd; Spellman, Jane L.; Nottmeier, Anna M.; Westerman, Drew A.; Blackstock, Joshua M.; Battreal, James L.

    2014-01-01

    Sixteen aquifers in Arkansas that currently serve or have served as sources of water supply are described with respect to existing groundwater protection and management programs, geology, hydrologic characteristics, water use, water levels, deductive analysis, projections of hydrologic conditions, and water quality. State and Federal protection and management programs are described according to regulatory oversight, management strategies, and ambient groundwater-monitoring programs that currently (2013) are in place for assessing and protecting groundwater resources throughout the State.

  5. Final Critical Habitat for the Arkansas River Shiner (Notropis girardi)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Arkansas River Shiner (Notropis girardi) occur. The geographic extent includes New...

  6. Central Arkansas National Wildlife Refuge Complex: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Central Arkansas NWR Complex for the next 15 years. This plan outlines the Complex...

  7. 76 FR 44031 - Arkansas; Major Disaster and Related Determinations

    Science.gov (United States)

    2011-07-22

    ....046, Fire Management Assistance Grant; 97.048, Disaster Housing Assistance to Individuals and...--Disaster Housing Operations for Individuals and Households; 97.050, Presidentially Declared Disaster... SECURITY Federal Emergency Management Agency Arkansas; Major Disaster and Related Determinations...

  8. [Arkansas annual winter goose survey: December 10 & 11, 1979

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, resident geese, and BlueSnow Geese are included.

  9. [Arkansas annual winter goose survey: December 11 - 15, 1978

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A table presents data gathered during the goose survey in Arkansas. Numbers of Canada Geese, Whitefronted Geese, resident geese, and BlueSnow Geese are included.

  10. Fitness-for-duty program at Arkansas nuclear one

    International Nuclear Information System (INIS)

    The key events at Arkansas Power and Light Co. (AP and L) the fitness for duty (FFD) program since January 1981 are outlined. The sequential interface and impact of the US Nuclear Regulatory Commission (NRC) ruling, of the Edison Electric Institute (EEI)-published guidelines, and of the NUMARC commitment have played a key role in the continuing evolution of the FFD program at Arkansas Nuclear One (ANO). The major elements of the FFD program, difficulties encountered, and successful results are described

  11. 78 FR 72877 - Arkansas Electric Corporation v. Oklahoma Gas and Electric Company; Notice of Complaint

    Science.gov (United States)

    2013-12-04

    ... Energy Regulatory Commission Arkansas Electric Corporation v. Oklahoma Gas and Electric Company; Notice... Procedure of the Federal Energy Regulatory Commission (Commission), 18 CFR 385.206, Arkansas Electric Corporation (Complainant) filed a formal complaint against Oklahoma Gas and Electric Company...

  12. 78 FR 48716 - Notice of Availability of the Final Environmental Impact Statement for the Arkansas Valley...

    Science.gov (United States)

    2013-08-09

    ... Arkansas River Basin, groundwater, climate change, recreation biological resources, human environment... Valley Conduit and Long-Term Excess Capacity Master Contract, Fryingpan-Arkansas Project; Bent, Chaffee... statement (EIS) for the Arkansas Valley Conduit (AVC) and Long-Term Excess Capacity Contract,...

  13. Suramin inhibits EV71 infection.

    Science.gov (United States)

    Wang, Yaxin; Qing, Jie; Sun, Yuna; Rao, Zihe

    2014-03-01

    Enterovirus-71 (EV71) is one of the major causative reagents for hand-foot-and-mouth disease. In particular, EV71 causes severe central nervous system infections and leads to numerous dead cases. Although several inactivated whole-virus vaccines have entered in clinical trials, no antiviral agent has been provided for clinical therapy. In the present work, we screened our compound library and identified that suramin, which has been clinically used to treat variable diseases, could inhibit EV71 proliferation with an IC50 value of 40 μM. We further revealed that suramin could block the attachment of EV71 to host cells to regulate the early stage of EV71 infection, as well as affected other steps of EV71 life cycle. Our results are helpful to understand the mechanism for EV71 life cycle and provide a potential for the usage of an approved drug, suramin, as the antiviral against EV71 infection.

  14. Arkansas Superintendents Predict Curriculum for the Year 2000.

    Science.gov (United States)

    Vaupel, Carl F., Jr.; Sweat, Joseph P.

    1985-01-01

    Superintendents of 164 Arkansas small school districts predicted the importance of 20 selected academic courses/physical activities by the year 2000. Courses/activities ranked highest for 2000 were computer science, physics, soccer, economics, and composition. Study assumes collective wisdom or bias of superintendents will alter future secondary…

  15. Arkansas and the Southern Regional Education Board, December 2014

    Science.gov (United States)

    Southern Regional Education Board (SREB), 2014

    2014-01-01

    This report details Arkansas's participation in SREB programs and services from December 2013 through November 2014. Appropriations from member states support SREB's core operations and general services. SREB leverages the long-standing commitment of member states to attract external funding for an array of targeted projects for educational…

  16. Black bears in Arkansas: Characteristics of a successful translocation

    Science.gov (United States)

    Smith, Kimberly G.; Clark, Joseph D.

    1994-01-01

    In 1958, the Arkansas Game and Fish Commission began translocating black bears (Ursus americanus) from Minnesota to the Interior Highlands (Ozark and Ouachita mountains) of Arkansas where bears had been extirpated early in this century. This project continued for 11 years with little public imput, during which time an estimated 254 bears were released. We estimate there are now >2,500 bears in the Interior Highlands of Arkansas, Missouri, and Oklahoma, making it one of the most successful translocations of a Carnivora. Factors that contributed to the success include use of wild-captured animals, elimination of major factors associated with extirpation, release into prime habitats within the former range, multiple release sites, release of 20–40 animals/year for eight years, and release of mostly males prior to release of mostly females. Studies on two allopatric populations demonstrate that they are now diverging in some demographic characteristics, including litter size, cub survivorship, and adult sex-ratio. Translocation of black bears to the Interior Highlands is successful in terms of numbers of animals, but it will not be truly successful until people accept black bears as part of the regional fauna. To that end, those associated with management and research of bears in Arkansas are now focussing on public education and control of nuisance bears.

  17. State Minimum Core Curricula: Arkansas Institutions of Higher Education.

    Science.gov (United States)

    Arkansas State Dept. of Higher Education, Little Rock.

    State minimum core curricula for two-year and four-year colleges and universities have been approved by the Arkansas Board of Higher Education. Within the framework of the State Minimum Core, each state institution is required to propose 35 semester/credit hours from its institutional general education core to be recognized for purposes of the…

  18. State Minimum Core Curricula: Arkansas Institutions of Higher Education, 2000.

    Science.gov (United States)

    Arkansas State Dept. of Higher Education, Little Rock.

    This document provides state minimum core curricula for each two- and four-year institution of higher education in Arkansas, determined by the Department of Higher Education. Courses within this core are to apply toward the general education core curriculum requirements for baccalaureate degrees at state-supported institutions and should be fully…

  19. State Minimum Core Curricula: Arkansas Institutions of Higher Education, 2003.

    Science.gov (United States)

    Arkansas State Dept. of Higher Education, Little Rock.

    In 1990 the Arkansas State Board of Higher Education adopted guidelines for the development of state minimum core curriculum response to state legislation. This legislation provides that courses within the core shall apply to the general education core curriculum requirements for baccalaureate degrees at state-supported institutions and shall be…

  20. Project CAP. Boston Mountains Educational Cooperative, Greenland, Arkansas.

    Science.gov (United States)

    Hamilton, Jack A.; Leffler, Jeanne

    This description of career education activities in Greenland, Arkansas, was prepared as part of a study conducted to identify evaluated, exemplary career education activities which represent the best of the current career education programs and practices referred to in Public Law 93-380. (See CE 018 212 for the final report of this study.) This…

  1. Arkansas Solar Retrofit Guide. Greenhouses, Air Heaters and Water Heaters.

    Science.gov (United States)

    Skiles, Albert; Rose, Mary Jo

    Solar retrofits are devices of structures designed to be attached to existing buildings to augment their existing heating sources with solar energy. An investigation of how solar retrofits should be designed to suit the climate and resources of Arkansas is the subject of this report. Following an introduction (section 1), section 2 focuses on…

  2. Lithology, hydrologic characteristics, and water quality of the Arkansas River Valley alluvial aquifer in the vicinity of Van Buren, Arkansas

    Science.gov (United States)

    Kresse, Timothy M.; Westerman, Drew A.; Hart, Rheannon M.

    2015-01-01

    A study to assess the potential of the Arkansas River Valley alluvial aquifer in the vicinity of Van Buren, Arkansas, as a viable source of public-supply water was conducted by the U.S. Geological Survey in cooperation with the Little Rock, District, U.S. Army Corps of Engineers. An important study component was to identify possible changes in hydrologic conditions following installation of James W. Trimble Lock and Dam 13 (December 1969) on the Arkansas River near the study area. Data were gathered for the study in regard to the lithology, hydrologic characteristics, and water quality of the aquifer. Lithologic information was obtained from drillers’ logs of wells drilled from 1957 through 1959. Water-quality samples were collected from 10 irrigation wells and analyzed for inorganic constituents and pesticides. To evaluate the potential viability of the alluvial aquifer in the Van Buren area, these data were compared to similar stratigraphic, lithologic, and groundwater-quality data from the Arkansas River Valley alluvial aquifer at Dardanelle, Ark., where the aquifer provides a proven, productive, sole-source of public-supply water.

  3. The Arkansas Autism Developmental Disabilities Monitoring (AR ADDM) project: statewide autism surveillance in a rural state.

    Science.gov (United States)

    Lopez, Maya; Schulz, Eldon G; Baroud, Thaer; Hudson, Allison; Wilson, Mark

    2012-03-01

    In 2002, the Arkansas Autism and Developmental Disabilities Monitoring (AR ADDM) project collected data on the number and characteristics of resident children aged 8 years using a retrospective record review standardized methodology. This paper provides a first-look epidemiology of ASDs among 8 year old Arkansas children using data from the 2002 study year. Overall prevalence estimates, demographic distribution and a temporal lag from concerns identified to diagnosis of ASDs among 8 year olds in Arkansas were similar to that in other sites. Dissemination of information that promotes timely resolution of developmental concerns and improving educational services will benefit children with autism in Arkansas. PMID:22479981

  4. Middle Claiborne Aquifer: Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Claiborne Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The...

  5. Floods of Selected Streams in Arkansas, Spring 2008

    Science.gov (United States)

    Funkhouser, Jaysson E.; Eng, Ken

    2009-01-01

    Floods can cause loss of life and extensive destruction to property. Monitoring floods and understanding the reasons for their occurrence are the responsibility of many Federal agencies. The National Weather Service, the U.S. Army Corps of Engineers, and the U.S. Geological Survey are among the most visible of these agencies. Together, these three agencies collect and analyze floodflow information to better understand the variety of mechanisms that cause floods, and how the characteristics and frequencies of floods vary with time and location. The U.S. Geological Survey (USGS) has monitored and assessed the quantity of streamflow in our Nation's streams since the agency's inception in 1879. Because of ongoing collection and assessment of streamflow data, the USGS can provide information about a range of surface-water issues including the suitability of water for public supply and irrigation and the effects of agriculture and urbanization on streamflow. As part of its streamflow-data collection activities, the USGS measured streamflow in multiple streams during extreme flood events in Arkansas in the spring of 2008. The analysis of streamflow information collected during flood events such as these provides a scientific basis for decision making related to resource management and restoration. Additionally, this information can be used by water-resource managers to better define flood-hazard areas and to design bridges, culverts, dams, levees, and other structures. Water levels (stage) and streamflow (discharge) currently are being monitored in near real-time at approximately 150 locations in Arkansas. The streamflow-gaging stations measure and record hydrologic data at 15-minute or hourly intervals; the data then are transmitted through satellites to the USGS database and displayed on the internet every 1 to 4 hours. Streamflow-gaging stations in Arkansas are part of a network of over 7,500 active streamflow-gaging stations operated by the USGS throughout the United

  6. UPPER BUFFALO WILDERNESS AND BUFFALO ADDITION ROADLESS AREA, ARKANSAS.

    Science.gov (United States)

    Miller, Mary H.; Armstrong, Michelle K.

    1984-01-01

    The Upper Buffalo Wilderness and Buffalo Addition Roadless Area covers about 19 sq mi in the Ozark National Forest, Newton County, Arkansas. No metal-bearing minerals were observed during geologic mapping, and analyses for zinc and lead contents in surface rock and sediment samples from the study area are not anomalous. Exploratory drilling into the Boone Formation and (or) the Everton Formation will be necessary to evaluate the mineral-resource potential of zinc and lead in the study area.

  7. Submarine-fan sedimentation, Ouachita Mountains, Arkansas and Oklahoma

    Energy Technology Data Exchange (ETDEWEB)

    Moiola, R.J.; Shanmugam, G.

    1984-09-01

    More than 10,000 m (32,808 ft) of interbedded sandstones and shales comprise the Upper Mississippian and Lower Pennsylvanian flysch succession (Stanley, Jackfork, Johns Valley, Atoka) in the Ouachita Mountains of Arkansas and Oklahoma. Deposited primarily by turbidity current and hemipelagic processes in bathyal and abyssal water depths, these strata formed major submarine-fan complexes that prograded in a westward direction along the axis of an elongate remnant ocean basin that was associated with the collision and suturing of the North American and African-South American plates. A longitudinal fan system is visualized as the depositional framework for these strata, which were deposited in a setting analogous to the modern Bengal fan of the Indian Ocean. Facies analysis of the Jackfork formation indicates that inner fan deposits are present in the vicinity of Little Rock, Arkansas; middle fan channel and interchannel deposits occur at DeGray Dam and Friendship, Arkansas; and outer fan depositional-lobe deposits are present in southeastern Oklahoma. Boulder-bearing units (olistostromes), many with exotic clasts, were shed laterally into the Ouachita basin. They occur throughout the flysch succession and in all fan environments (i.e., inner, middle, and outer). This relationship may serve as a useful criterion for recognizing analogous longitudinal fan systems in the rock record.

  8. 78 FR 78398 - Notice and Request for Comments: LSC merger of the migrant service areas in Texas, Arkansas...

    Science.gov (United States)

    2013-12-26

    ... Notice and Request for Comments: LSC merger of the migrant service areas in Texas, Arkansas, Kentucky... Request for Comments--LSC merger of the migrant service areas in Texas, Arkansas, Kentucky, Louisiana..., Arkansas, Kentucky, Louisiana, Mississippi, Tennessee, and Alabama migrant service areas. Grants for...

  9. 78 FR 27306 - Radio Broadcasting Services; Dermott, Arkansas, and Cleveland, Mississippi

    Science.gov (United States)

    2013-05-10

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Dermott, Arkansas, and Cleveland, Mississippi AGENCY... Delta Radio Network, LLC, substitutes FM Channel 224A for 289A at Dermott, Arkansas, and substitutes FM...-20110913AAK. Channel 224A can be allotted at Dermott with a site restriction of 3.5 km (2.2 miles)...

  10. "McLean v. Arkansas" (1982) and Beyond: Implications for Biology Professors

    Science.gov (United States)

    Bland, Mark W.; Moore, Randy

    2011-01-01

    To assess current trends of evolution instruction in high schools of the mid-South, we invited Arkansas high school biology teachers from across the state to respond to a survey designed to address this issue. We also asked students enrolled in a freshman-level, nonmajors biology course at a midsize public Arkansas university to recall their…

  11. Association of School-Based Influenza Vaccination Clinics and School Absenteeism--Arkansas, 2012-2013

    Science.gov (United States)

    Gicquelais, Rachel E.; Safi, Haytham; Butler, Sandra; Smith, Nathaniel; Haselow, Dirk T.

    2016-01-01

    Background: Influenza is a major cause of seasonal viral respiratory illness among school-aged children. Accordingly, the Arkansas Department of Health (ADH) coordinates >800 school-based influenza immunization clinics before each influenza season. We quantified the relationship between student influenza vaccination in Arkansas public schools…

  12. 77 FR 6711 - Approval and Promulgation of Implementation Plans; Arkansas; Infrastructure Requirements for the...

    Science.gov (United States)

    2012-02-09

    ... proposing to approve SIP revisions that modify the Arkansas PSD SIP to include nitrogen oxides (NO X ) as an... Greenhouse Gas Emitting-Sources in State Implementation Plans; Final Rule,'' 75 FR 82536 (December 30, 2010... Greenhouse Gas Tailoring Rule; Final Rule.'' 75 FR 31514 (June 3, 2010). The approved Arkansas SIP...

  13. 77 FR 47779 - Arkansas: Final Authorization of State Hazardous Waste Management Program Revision

    Science.gov (United States)

    2012-08-10

    ... FR 31756-31769 Arkansas Code of 1987 Annotated (A.C.A.) Treatment Sludges from Auto Sections 8-7-201... AGENCY 40 CFR Part 271 Arkansas: Final Authorization of State Hazardous Waste Management Program Revision... to the EPA for Final authorization of the changes to its hazardous waste program under the...

  14. The Arkansas Building Industry's Perceptions of Entry-Level Employee Work Competencies.

    Science.gov (United States)

    Gregson, James A.

    1989-01-01

    A study surveyed 100 (66 responses) employers in the Arkansas home building industry to determine the emphasis they place on affective, cognitive, and psychomotor skills. Although employers value affective work behaviors, the Arkansas building trades competency profiles used for curriculum development emphasize the psychomotor domain. (JOW)

  15. First isolation of enterovirus 71 (EV-71 from Northern Brazil

    Directory of Open Access Journals (Sweden)

    Lamarão Leticia Martins

    2003-01-01

    Full Text Available Enterovirus 71 (EV-71 has been associated to cases of neurological disease in many countries including Brazil. This virus has now been reported from three of the five Brazilian regions. Our study relates the findings concerning to the first isolate of this virus in Northern region of Brazil. A 15-month old female patient, from the rural zone of the municipality of Santana do Araguaia in southern Pará state was admitted at the hospital with acute, flaccid, asymmetric and ascending motor deficiency, located in the right lower limb. Stools samples from this child were inoculated in RD cells and was isolated an EV-71. We plan to sequence our strain and compare it to other isolates in Brazil. Differences at the molecular level can explain why EV-71 strains circulating in other continents, such as Asia, appear to be more virulent.

  16. Status of Water Levels in Aquifers in the Nacatoch Sand of Southwestern and Northeastern Arkansas and the Tokio Formation of Southwestern Arkansas, February 2005

    Science.gov (United States)

    Schrader, T.P.

    2007-01-01

    The Nacatoch Sand and Tokio Formation aquifers in southwestern Arkansas and the Nacatoch Sand aquifer in northeastern Arkansas are sources of water for industrial, public supply, domestic, and agricultural uses. Potentiometric-surface maps were constructed from water-level measurements made in 60 wells completed in the Nacatoch Sand and 50 wells completed in the Tokio Formation during February 2005. Aquifers in the Nacatoch Sand and Tokio Formation are hereafter referred to as the Nacatoch aquifer and Tokio aquifer, respectively. The direction of ground-water flow in the Nacatoch aquifer in northeastern Arkansas generally is towards the southeast. The potentiometric high is located along the north and northwestern boundaries of the area. The direction of ground-water flow in the Nacatoch aquifer in southwestern Arkansas is towards the south-southeast in Little River, Miller, and Hempstead Counties and to the east-southeast in Nevada and Clark Counties. The potentiometric high is located within the outcrop area in north-central Hempstead County. A cone of depression exists in the Nacatoch aquifer at Hope in southeastern Hempstead County. The direction of ground-water flow in the Tokio aquifer in southwestern Arkansas generally is towards the south or southeast. The potentiometric high is within the outcrop area. An area of artesian flow exists in southeastern Pike, northeastern Hempstead, and northwestern Nevada Counties. One apparent cone of depression might exist northwest of Hope in Hempstead County. In northeastern Arkansas, withdrawals from the Nacatoch aquifer increased by 516 percent from 1965 to 2000. In southwestern Arkansas, withdrawals from Nacatoch aquifer and Tokio aquifer increased by 125 percent and 201 percent, respectively, from 1965 to 1980 and decreased by 93 percent and 80 percent, respectively, from 1980 to 2000. Long-term hydrographs were prepared for 10 wells in the study areas. Changes in water levels in some wells may be associated with

  17. Status of Water Levels in Aquifers in the Nacatoch Sand of Southwestern and Northeastern Arkansas and the Tokio Formation of Southwestern Arkansas, 1999

    Science.gov (United States)

    Schrader, Tony P.

    1999-01-01

    Nacatoch Sand and Tokio Formation aquifers in southwestern Arkansas and the Nacatoch Sand aquifer in northeastern Arkansas are a source of water for industrial, public supply, domestic, and agricultural uses. Potentiometric-surface maps were constructed from water-level measurements made in 59 wells completed in the Nacatoch Sand and 45 wells completed in the Tokio Formation from April through May 1999. The direction of ground-water flow in the aquifer in the Nacatoch Sand in northeastern Arkansas generally is towards the southeast. The potentiometric high is located along the north and northwestern boundaries. The direction of ground-water flow in the aquifer in the Nacatoch Sand in southwestern Arkansas is towards the south-southeast in Little River, Miller, and Hempstead Counties and to the east-southeast in Nevada and Clark Counties. The potentiometric high is located within the outcrop area in north-central Hempstead County. A cone of depression exists in the aquifer in the Nacatoch Sand at Hope in southeastern Hempstead County. The direction of ground-water flow in the aquifer in the Yokio Formation in southwestern Arkansas generally is towards the southeast. The potentiometric high is within the outcrop area. An area of artesian flow exists in southeastern Pike, northeastern Hempstead, and northwestern Nevada Counties. One apparent cone of depression might exist northwest of Hope in Hempstead County. In northeastern Arkansas, withdrawals from the Nacatoch Sand increased by 736 percent from 1965 to 1995. In southwestern Arkansas withdrawals from aquifers in the Nacatoch Sand and Yokio Formation increased by 125 percent and 201 percent, respectively, from 1965 to 1980 and decreased by 78 percent and 63 percent, respectively, from 1980 to 1995. Long-term hydrographs were prepared for 13 wells in the study area. Changes in water levels in some wells may be associated with changes in withdrawals from the respective aquifers.

  18. Dry season mean monthly flow and harmonic mean flow regression equations for selected ungaged basins in Arkansas

    Science.gov (United States)

    Breaker, Brian K.

    2015-01-01

    The U.S. Geological Survey, in cooperation with the Arkansas Department of Environmental Quality, Southwestern Energy, the Arkansas Natural Resources Commission, and the Arkansas Game and Fish Commission, developed regression equations for estimation of dry season mean monthly flows and harmonic mean flows that are representative of natural streamflow conditions at selected ungaged basins in Arkansas. Observed values of dry season mean monthly flow and harmonic mean flow computed from daily-mean flow data were used with basin characteristics to identify significant explanatory variables for multiple-linear-regression equations to estimate predicted values of dry season mean monthly flow and harmonic mean flow. Five dry season mean monthly flow regression equations and two harmonic mean flow regression equations were developed using dry season mean monthly flows and harmonic mean flows established for 91 and 93 U.S. Geological Survey continuous-record streamflow-gaging stations, respectively. The dry season in Arkansas is defined as the months of July through November for this study. For harmonic mean flow calculations and regression equations, the study area is composed of the Springfield-Salem Plateaus (Arkansas and Missouri), Boston Mountains, Arkansas Valley, Ouachita Mountains (Arkansas and Oklahoma), and West Gulf Coastal Plain (Arkansas) physiographic sections. All continuous-record streamflow-gaging stations used to compute dry season mean monthly flows were located within Arkansas.

  19. Structure determination of enterovirus 71

    Energy Technology Data Exchange (ETDEWEB)

    Plevka, Pavel; Perera, Rushika; Cardosa, Jane; Kuhn, Richard J.; Rossmann, Michael G. (Purdue); (Sentinext)

    2013-02-20

    Enterovirus 71 is a picornavirus that causes hand, foot and mouth disease but may induce fatal neurological illness in infants and young children. Enterovirus 71 crystallized in a body-centered orthorhombic space group with two particles in general orientations in the crystallographic asymmetric unit. Determination of the particle orientations required that the locked rotation function excluded the twofold symmetry axes from the set of icosahedral symmetry operators. This avoided the occurrence of misleading high rotation-function values produced by the alignment of icosahedral and crystallographic twofold axes. Once the orientations and positions of the particles had been established, the structure was solved by molecular replacement and phase extension.

  20. 77 FR 55430 - Arkansas Regulatory Program and Abandoned Mine Land Reclamation Plan

    Science.gov (United States)

    2012-09-10

    ... grammatical changes, correct punctuation, revise dates, and delete and add citations. The proposed amendment consists of substantive changes to Arkansas's regulations regarding: Subchapter A--General; Subchapter F... grammatical changes, correct punctuation, revise dates, update addresses, and delete and add...

  1. Arkansas State Briefing Book for low-level radioactive waste management

    International Nuclear Information System (INIS)

    The Arkansas State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. This report contains a profile of low-level radioactive waste generators in Arkansas. The profile is the result of a survey of NRC licensees in Arkansas. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Arkansas

  2. Mississippi River Valley Alluvial Aquifer, Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, Tennessee; 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Outcrop and subcrop extent of the Mississippi River Valley Alluvial Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, and Tennessee.

  3. Arkansas State Briefing Book for low-level radioactive-waste management

    International Nuclear Information System (INIS)

    The Arkansas State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Arkansas. The profile is the result of a survey of NRC licensees in Arkansas. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Arkansas

  4. Middle Wilcox Aquifer: Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Wolcox Aquifer in Alabama, Arkansas, Illinois, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The hydrogeologic...

  5. Upper Claiborne Aquifer: Alabama, Arkansas, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Upper Claiborne Aquifer in Arkansas, Alabama, Louisiana, Missouri, Mississippi, and Tennessee. The hydrogeologic unit dataset...

  6. Middle Claiborne Confining Unit: Alabama, Arkansas, Kentucky, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Middle Claiborne Confining Unit in Alabama, Arkansas, Kentucky, Louisiana, Missouri, Mississippi, Tennessee. The hydrogeologic...

  7. An Evaluation of Ecosystem Restoration Options for Grand Prairie Region of Arkansas

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Evaluation of Ecosystem Restoration and Management Options covers the hydrogeomorphic analysis (HGM) for Grand Prairie Region of Arkansas. This three step...

  8. Vicksburg-Jackson Confining Unit: Alabama, Arkansas, Louisiana, Missouri, Mississippi, Tennessee 2006-2008

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Digital hydrogeologic surface of the Vicksburg-Jackson Confining Unit in Arkansas, Alabama, Louisiana, Missouri, Mississippi, and Tennessee. The hydrogeologic unit...

  9. Employment Discrimination Based on Sexual Orientation and Gender Identity in Arkansas

    OpenAIRE

    Mallory, Christy; Sears, Brad

    2015-01-01

    None of the approximately 47,000 LGBT workers in Arkansas are explicitly protected from discrimination under local, state or federal laws. If sexual orientation and gender identity were added to existing statewide non-discrimination laws, 22 more complaints would be filed in Arkansas each year. The cost of enforcing those complaints would be negligible, and would not require additional court or administrative staff.

  10. Status of Water Levels and Selected Water-Quality Conditions in the Mississippi River Valley Alluvial Aquifer in Eastern Arkansas, 2004

    Science.gov (United States)

    Schrader, T.P.

    2006-01-01

    analysis of long-term water-level changes (1980-2004) in the depression in Arkansas and Prairie Counties shows the effects of the elongation of this depression. Water samples were collected from 138 wells completed in the alluvial aquifer and measured onsite for specific conductance and temperature. Samples were collected at 71 wells for dissolved chloride analysis at the U.S. Geological Survey National Water Quality Laboratory. Specific conductance ranged from 205 microsiemens per centimeter at 25 degrees Celsius at a well in Lonoke County to 1,440 microsiemens per centimeter at 25 degrees Celsius at a well in Monroe County.

  11. Folic acid and the decline in neural tube defects in Arkansas.

    Science.gov (United States)

    Mosley, Bridget S; Hobbs, Charlotte A; Flowers, Bettye S; Smith, Veronica; Robbins, James M

    2007-04-01

    Folic acid has been shown to reduce the risk of pregnancies affected by neural tube defects (NTDs) by as much as 70%. Cereal grains sold in the U.S. have been fortified with folic acid since 1998. The Arkansas Reproductive Health Monitoring System and the Arkansas Folic Acid Coalition have encouraged use of folic acid and monitored the impact of increased consumption of folic acid among Arkansans. NTDs in Arkansas have declined 40% since intervention programs were implemented. The greatest decline has been observed among white and Hispanic women. Efforts to encourage folic acid consumption should continue to target Arkansas women. NTDs include anencephaly and spina bifida. These birth defects result from incomplete closure of the fetal neural tube during the first month of pregnancy. Infants with anencephaly are born without all or most of their brain and die within a few days of life. Infants with spina bifida have varying degrees of impairment ranging from little noticeable disability to severe, lifelong disability. Folic acid, when taken in supplement form has been shown to reduce the risk of a pregnancy affected by a neural tube defect by as much as 70%. As a result of this finding, the U.S. Federal Drug Administration mandated that cereal grains sold in this country be fortified with at least 140 mcg of folic acid per 100 grams of grain by January 1, 1998. Prior to mandatory fortification, the March of Dimes and the U.S. Public Health Service released statements encouraging all women of reproductive age who are capable of becoming pregnant to take 400 mcg 'of synthetic folic acid daily. The Arkansas Reproductive Health Monitoring System (ARHMS) has monitored rates of NTDs in Arkansas since 1980. ARHMS is the lead agency of the Arkansas Folic Acid Coalition whose mission is to encourage folic acid use among all Arkansas women of reproductive age. In this report, we summarize efforts by ARHMS and the Arkansas Folic Acid Coalition to increase the awareness and

  12. Folic acid and the decline in neural tube defects in Arkansas.

    Science.gov (United States)

    Mosley, Bridget S; Hobbs, Charlotte A; Flowers, Bettye S; Smith, Veronica; Robbins, James M

    2007-04-01

    Folic acid has been shown to reduce the risk of pregnancies affected by neural tube defects (NTDs) by as much as 70%. Cereal grains sold in the U.S. have been fortified with folic acid since 1998. The Arkansas Reproductive Health Monitoring System and the Arkansas Folic Acid Coalition have encouraged use of folic acid and monitored the impact of increased consumption of folic acid among Arkansans. NTDs in Arkansas have declined 40% since intervention programs were implemented. The greatest decline has been observed among white and Hispanic women. Efforts to encourage folic acid consumption should continue to target Arkansas women. NTDs include anencephaly and spina bifida. These birth defects result from incomplete closure of the fetal neural tube during the first month of pregnancy. Infants with anencephaly are born without all or most of their brain and die within a few days of life. Infants with spina bifida have varying degrees of impairment ranging from little noticeable disability to severe, lifelong disability. Folic acid, when taken in supplement form has been shown to reduce the risk of a pregnancy affected by a neural tube defect by as much as 70%. As a result of this finding, the U.S. Federal Drug Administration mandated that cereal grains sold in this country be fortified with at least 140 mcg of folic acid per 100 grams of grain by January 1, 1998. Prior to mandatory fortification, the March of Dimes and the U.S. Public Health Service released statements encouraging all women of reproductive age who are capable of becoming pregnant to take 400 mcg 'of synthetic folic acid daily. The Arkansas Reproductive Health Monitoring System (ARHMS) has monitored rates of NTDs in Arkansas since 1980. ARHMS is the lead agency of the Arkansas Folic Acid Coalition whose mission is to encourage folic acid use among all Arkansas women of reproductive age. In this report, we summarize efforts by ARHMS and the Arkansas Folic Acid Coalition to increase the awareness and

  13. The fishes of Hot Springs National Park, Arkansas, 2003

    Science.gov (United States)

    Petersen, James C.; Justus, B.G.

    2005-01-01

    Fish communities were sampled from eight sites within Hot Springs National Park. Fish were collected by seining and electrofishing during base-flow periods in July and October 2003. All individuals were identified to species. More than 1,020 individuals were collected, representing 24 species. The number of species collected at the sites ranged from 5 to 19. Central stoneroller, orangebelly darter, and longear sunfish were among the more abundant fish species at most sites. These species are typical of small streams in this area. An expected species list incorrectly listed 35 species because of incorrect species range or habitat requirements. Upon revising this list, the inventory yielded 24 of the 51 expected species (47 percent). No species collected in 2003 were federally-listed threatened or endangered species. However, two species collected at Hot Springs National Park may be of special interest to National Park Service managers and others. The Ouachita madtom is endemic to the Ouachita Mountains and is listed as a species of special concern by the Arkansas Natural Heritage Commission. The grass carp, which is a native of eastern Asia, is present in Ricks Pond; one individual was collected and no other grass carp were observed. The introduction of grass carp into the United States is a controversial issue because of possible (but undocumented) harmful effects on native species and habitats.

  14. Ooid mineralogy and diagenesis of upper Mississippian Pitkin Formation, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Heydari, E.; Snelling, R.D.; Dawson, W.C.; Machain, M.L. (Louisiana State Univ., Baton Rouge (USA))

    1989-08-01

    The Pitkin formation is a marine oolitic-bioclastic limestone exhibiting shoal, lagoonal, beach, and tidal-channel facies. Ooids formed on the Pitkin carbonate shelf show lateral and vertical variations in original mineralogy. In a section south of Mountain View, Arkansas, the lower marine shoal and shoreface facies are composed of originally aragonitic ooids. These ooids are replaced by neomorphic calcite and exhibit elevated strontium and low magnesium concentrations. The overlying lagoonal facies are composed of originally calcite ooids that have retained their original radial fabrics. These ooids exhibit low strontium and high magnesium concentration. Ooids in sections to the west of Mountain View are all calcitic. Early diagenesis of the formation is dominated by marine cementation. Postdepositional diagenesis encompasses extensive dissolution of originally aragonite and magnesian calcite components, precipitation of a late unzoned ferroan calcite, and minor amounts of saddle dolomite. Grainstone units lack any intergranular porosity. Pressure solution contacts among grains in grainstones are generally uncommon. Cementation alone is responsible for destruction of all of the original porosity in these rocks. Intensive pressure solution is observed only in packstone units not affected by early marine cementation.

  15. 16 CFR 1.71 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Administration. 1.71 Section 1.71 Commercial Practices FEDERAL TRADE COMMISSION ORGANIZATION, PROCEDURES AND RULES OF PRACTICE GENERAL PROCEDURES Administration of the Fair Credit Reporting Act § 1.71 Administration. The general administration of the...

  16. 27 CFR 71.83 - Evidence.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Evidence. 71.83 Section 71... General § 71.83 Evidence. Any evidence which would be admissible under the rules of evidence governing... his case or defense by oral or documentary evidence, depositions, duly authenticated copies of...

  17. 25 CFR 163.71 - Agreement funding.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Agreement funding. 163.71 Section 163.71 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER GENERAL FORESTRY REGULATIONS Cooperative Agreements § 163.71 Agreement funding. In cooperative agreements, the Secretary is authorized to advance...

  18. 28 CFR 71.25 - Fees.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Fees. 71.25 Section 71.25 Judicial... REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71.25 Fees. The party requesting a subpoena shall pay the cost of the fees and mileage of any witness subpoenaed in...

  19. 10 CFR 71.123 - Test control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Test control. 71.123 Section 71.123 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.123 Test control. The licensee, certificate holder, and applicant for a CoC shall establish a...

  20. 46 CFR 71.01-5 - Posting.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Posting. 71.01-5 Section 71.01-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS INSPECTION AND CERTIFICATION Certificate of Inspection § 71.01-5 Posting. The certificate of inspection shall be displayed under glass in a...

  1. 46 CFR 71.05-15 - Posting.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Posting. 71.05-15 Section 71.05-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS INSPECTION AND CERTIFICATION Permit To Proceed to Another Port for Repair § 71.05-15 Posting. (a) The permit shall be carried in a manner similar...

  2. 46 CFR 71.10-15 - Posting.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Posting. 71.10-15 Section 71.10-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS INSPECTION AND CERTIFICATION Permit To Engage in Excursions § 71.10-15 Posting. (a) The permit when used, shall be carried in addition to...

  3. 36 CFR 71.4 - Posting.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Posting. 71.4 Section 71.4... § 71.4 Posting. (a) The administering bureaus of the Department of the Interior shall provide for the posting of the following designation sign at entrances to Designated Entrance Fee Areas and at...

  4. 7 CFR 905.71 - Other information.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Other information. 905.71 Section 905.71 Agriculture... TANGELOS GROWN IN FLORIDA Order Regulating Handling Handlers' Reports § 905.71 Other information. Upon... committee, in such manner and at such times as it prescribes, such other information as will enable it...

  5. 29 CFR 71.15 - Training.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Training. 71.15 Section 71.15 Labor Office of the Secretary... § 71.15 Training. All DOL systems managers, disclosure officers, and employees with responsibilities under the Privacy Act shall periodically attend training offered by the Department on the Privacy Act....

  6. 40 CFR 68.71 - Training.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Training. 68.71 Section 68.71... ACCIDENT PREVENTION PROVISIONS Program 3 Prevention Program § 68.71 Training. (a) Initial training. (1... operating a newly assigned process, shall be trained in an overview of the process and in the...

  7. 22 CFR 41.71 - Transit aliens.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Transit aliens. 41.71 Section 41.71 Foreign... NATIONALITY ACT, AS AMENDED Transit Aliens § 41.71 Transit aliens. (a) Transit aliens—general. An alien is classifiable as a nonimmigrant transit alien under INA 101(a) (15) (C) if the consular officer is...

  8. 7 CFR 985.71 - Effective time.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Effective time. 985.71 Section 985.71 Agriculture....71 Effective time. The provisions of this subpart, and of any amendment thereto, shall become effective at such time as the Secretary may declare and shall continue in force until terminated...

  9. 50 CFR 25.71 - Public safety.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Public safety. 25.71 Section 25.71... NATIONAL WILDLIFE REFUGE SYSTEM ADMINISTRATIVE PROVISIONS Safety Regulations § 25.71 Public safety. Persons using national wildlife refuges shall comply with the safety requirements which are established...

  10. 49 CFR 71.8 - Mountain zone.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Mountain zone. 71.8 Section 71.8 Transportation Office of the Secretary of Transportation STANDARD TIME ZONE BOUNDARIES § 71.8 Mountain zone. The fourth zone, the mountain standard time zone, includes that part of the United States that is west of...

  11. 14 CFR 437.71 - Flight rules.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Flight rules. 437.71 Section 437.71... TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.71 Flight rules. (a) Before initiating rocket-powered flight, a permittee must confirm that all systems and operations necessary to ensure...

  12. 10 CFR 34.71 - Utilization logs.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Utilization logs. 34.71 Section 34.71 Energy NUCLEAR... RADIOGRAPHIC OPERATIONS Recordkeeping Requirements § 34.71 Utilization logs. (a) Each licensee shall maintain utilization logs showing for each sealed source the following information: (1) A description, including...

  13. 30 CFR 71.702 - Asbestos standard.

    Science.gov (United States)

    2010-07-01

    ...) using the OSHA Reference Method in OSHA's asbestos standard found in 29 CFR 1910.1001, Appendix A, or a... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Asbestos standard. 71.702 Section 71.702... Contaminants § 71.702 Asbestos standard. (a) Definitions. Asbestos is a generic term for a number...

  14. 10 CFR 71.37 - Quality assurance.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Quality assurance. 71.37 Section 71.37 Energy NUCLEAR... Package Approval § 71.37 Quality assurance. (a) The applicant shall describe the quality assurance program... quality assurance program that are applicable to the particular package design under...

  15. 7 CFR 1412.71 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Administration. 1412.71 Section 1412.71 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF...) Program § 1412.71 Administration. (a) All of the provisions of this part apply to this subpart. To...

  16. 7 CFR 7.1 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Administration. 7.1 Section 7.1 Agriculture Office of the Secretary of Agriculture SELECTION AND FUNCTIONS OF AGRICULTURAL STABILIZATION AND CONSERVATION STATE, COUNTY AND COMMUNITY COMMITTEES § 7.1 Administration. (a) The regulations of this part...

  17. 10 CFR 820.71 - Standard.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Standard. 820.71 Section 820.71 Energy DEPARTMENT OF ENERGY PROCEDURAL RULES FOR DOE NUCLEAR ACTIVITIES Criminal Penalties § 820.71 Standard. If a person subject to the Act or the DOE Nuclear Safety Requirements has, by act or omission, knowingly and...

  18. 21 CFR 1316.71 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... Substances Act (84 Stat. 1242; 21 U.S.C. 801) and/or the Controlled Substances Import and Export Act (84 Stat... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Definitions. 1316.71 Section 1316.71 Food and... PROCEDURES Seizure, Forfeiture, and Disposition of Property § 1316.71 Definitions. As used in this...

  19. 10 CFR 39.71 - Security.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Security. 39.71 Section 39.71 Energy NUCLEAR REGULATORY COMMISSION LICENSES AND RADIATION SAFETY REQUIREMENTS FOR WELL LOGGING Security, Records, Notifications § 39.71 Security. (a) A logging supervisor must be physically present at a temporary jobsite...

  20. 7 CFR 993.71 - Confidential information.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Confidential information. 993.71 Section 993.71... CALIFORNIA Order Regulating Handling Reports and Books and Other Records § 993.71 Confidential information... information constituting a trade secret or disclosing of the trade position, financial condition, or...

  1. 28 CFR 71.21 - Discovery.

    Science.gov (United States)

    2010-07-01

    ... REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71.21 Discovery. (a) The following types of discovery are authorized: (1) Requests for production of documents for... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Discovery. 71.21 Section 71.21...

  2. 10 CFR 71.35 - Package evaluation.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Package evaluation. 71.35 Section 71.35 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Application for Package Approval § 71.35 Package evaluation. The application must include the following: (a)...

  3. 10 CFR 71.33 - Package description.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Package description. 71.33 Section 71.33 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Application for Package Approval § 71.33 Package description. The application must include a description of the...

  4. Nutrient Concentrations, Loads, and Yields in the Eucha-Spavinaw Basin, Arkansas and Oklahoma, 2002-2006

    Science.gov (United States)

    Tortorelli, Robert L.

    2008-01-01

    The City of Tulsa, Oklahoma, uses Lake Eucha and Spavinaw Lake in the Eucha-Spavinaw basin in northwestern Arkansas and northeastern Oklahoma for public water supply. Taste and odor problems in the water attributable to blue-green algae have increased in frequency. Changes in the algae community in the lakes may be attributable to increases in nutrient levels in the lakes, and in the waters feeding the lakes. The U.S. Geological Survey, in cooperation with the City of Tulsa, investigated and summarized nitrogen and phosphorus concentrations and provided estimates of nitrogen and phosphorus loads, yields, and flow-weighted concentrations in the Eucha-Spavinaw basin for three 3-year periods - 2002-2004, 2003-2005, and 2004-2006, to update a previous report that used data from water-quality samples for a 3-year period from January 2002 through December 2004. This report provides information needed to advance knowledge of the regional hydrologic system and understanding of hydrologic processes, and provides hydrologic data and results useful to multiple agencies for interstate agreements. Nitrogen and phosphorus concentrations were significantly greater in runoff samples than in base-flow samples for all three periods at Spavinaw Creek near Maysville, Arkansas; Spavinaw Creek near Colcord, Oklahoma, and Beaty Creek near Jay, Oklahoma. Runoff concentrations were not significantly greater than base-flow concentrations at Spavinaw Creek near Cherokee, Arkansas; and Spavinaw Creek near Sycamore, Oklahoma except for phosphorus during 2003-2005. Nitrogen concentrations in base-flow samples significantly increased downstream in Spavinaw Creek from the Maysville to Sycamore stations then significantly decreased from the Sycamore to the Colcord stations for all three periods. Nitrogen in base-flow samples from Beaty Creek was significantly less than in samples from Spavinaw Creek. Phosphorus concentrations in base-flow samples significantly increased from the Maysville to

  5. Geologic map of the Murray Quadrangle, Newton County, Arkansas

    Science.gov (United States)

    Hudson, Mark R.; Turner, Kenzie J.

    2016-07-06

    This map summarizes the geology of the Murray quadrangle in the Ozark Plateaus region of northern Arkansas. Geologically, the area is on the southern flank of the Ozark dome, an uplift that has the oldest rocks exposed at its center, in Missouri. Physiographically, the Murray quadrangle is within the Boston Mountains, a high plateau region underlain by Pennsylvanian sandstones and shales. Valleys of the Buffalo River and Little Buffalo River and their tributaries expose an approximately 1,600-ft-thick (488-meter-thick) sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. The Buffalo National River, a park that encompasses the Buffalo River and adjacent land that is administered by the National Park Service is present at the northwestern edge of the quadrangle.Mapping for this study was carried out by field inspection of numerous sites and was compiled as a 1:24,000 geographic information system (GIS) database. Locations and elevation of sites were determined with the aid of a global positioning satellite receiver and a hand-held barometric altimeter that was frequently recalibrated at points of known elevation. Hill-shade relief and slope maps derived from a U.S. Geological Survey 10-meter digital elevation model as well as orthophotographs were used to help trace ledge-forming units between field traverses within the Upper Mississippian and Pennsylvanian part of the stratigraphic sequence. Strike and dip of beds were typically measured along stream drainages or at well-exposed ledges. Structure contours, constructed on the top of the Boone Formation and the base of a prominent sandstone unit within the Bloyd Formation, were drawn based on the elevations of field sites on these contacts well as other limiting information for their minimum elevations above hilltops or their maximum elevations below valley bottoms.

  6. HEK293 cells express dystrophin Dp71 with nucleus-specific localization of Dp71ab.

    Science.gov (United States)

    Nishida, Atsushi; Yasuno, Sato; Takeuchi, Atsuko; Awano, Hiroyuki; Lee, Tomoko; Niba, Emma Tabe Eko; Fujimoto, Takahiro; Itoh, Kyoko; Takeshima, Yasuhiro; Nishio, Hisahide; Matsuo, Masafumi

    2016-09-01

    The dystrophin gene consists of 79 exons and encodes tissue-specific isoforms. Mutations in the dystrophin gene cause Duchenne muscular dystrophy, of which a substantial proportion of cases are complicated by non-progressive mental retardation. Abnormalities of Dp71, an isoform transcribed from a promoter in intron 62, are a suspected cause of mental retardation. However, the roles of Dp71 in human brain have not been fully elucidated. Here, we characterized dystrophin in human HEK293 cells with the neuronal lineage. Reverse transcription-PCR amplification of the full-length dystrophin transcript revealed the absence of fragments covering the 5' part of the dystrophin cDNA. In contrast, fragments covering exons 64-79 were present. The Dp71 promoter-specific exon G1 was shown spliced to exon 63. We demonstrated that the Dp71 transcript comprised two subisoforms: one lacking exon 78 (Dp71b) and the other lacking both exons 71 and 78 (Dp71ab). Western blotting of cell lysates using an antibody against the dystrophin C-terminal region revealed two bands, corresponding to Dp71b and Dp71ab. Immunohistochemical examination with the dystrophin antibody revealed scattered punctate signals in the cytoplasm and the nucleus. Western blotting revealed one band corresponding to Dp71b in the cytoplasm and two bands corresponding to Dp71b and Dp71ab in the nucleus, with Dp71b being predominant. These results indicated that Dp71ab is a nucleus-specific subisoform. We concluded that Dp71, comprising Dp71b and Dp71ab, was expressed exclusively in HEK293 cells and that Dp71ab was specifically localized to the nucleus. Our findings suggest that Dp71ab in the nucleus contributes to the diverse functions of HEK293 cells.

  7. Milrinone in Enterovirus 71 Brain Stem Encephalitis.

    Science.gov (United States)

    Wang, Shih-Min

    2016-01-01

    Enterovirus 71 (EV71) was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD) across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE) is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Milrinone is a type III phosphodiesterase inhibitor that has both inotropic and vasodilator effects. Its clinical efficacy has been shown by modulating inflammation, reducing sympathetic over-activity, and improving survival in patients with EV71-associated PE. Milrinone exhibits immunoregulatory and anti-inflammatory effects in the management of systemic inflammatory responses in severe EV71 infection. PMID:27065870

  8. Milrinone in Enterovirus 71 Brain Stem Encephalitis

    Science.gov (United States)

    Wang, Shih-Min

    2016-01-01

    Enterovirus 71 (EV71) was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD) across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE) is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Milrinone is a type III phosphodiesterase inhibitor that has both inotropic and vasodilator effects. Its clinical efficacy has been shown by modulating inflammation, reducing sympathetic over-activity, and improving survival in patients with EV71-associated PE. Milrinone exhibits immunoregulatory and anti-inflammatory effects in the management of systemic inflammatory responses in severe EV71 infection. PMID:27065870

  9. Milrinone in Enterovirus 71 Brain Stem Encephalitis

    Directory of Open Access Journals (Sweden)

    SHIH-MIN eWANG

    2016-03-01

    Full Text Available Enterovirus 71 (EV71 was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Milrinone is a type III phosphodiesterase inhibitor that has both inotropic and vasodilator effects. Its clinical efficacy has been shown by modulating inflammation, reducing sympathetic over-activity, and improving survival in patients with EV71-associated PE. Milrinone exhibits immunoregulatory and anti-inflammatory effects in the management of systemic inflammatory responses in severe EV71 infection.

  10. Sample Curriculum Model, Grade 1, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 1 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  11. Sample Grade Level Benchmarks, Grades 5-8, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document presents the application and use of mathematics learning proposed by the Arkansas curriculum frameworks for grades 5-8. The standards are presented in chart form and organized into five strands: (1) number sense, properties, and operations; (2) geometry and spatial sense; (3) measurement; (4) data analysis, statistics, and…

  12. Sample Grade Level Benchmarks, Grades K-4, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document presents the application and use of mathematics learning proposed by the Arkansas curriculum frameworks for grades K-4. The standards are presented in chart form and organized into five strands: (1) number sense, properties, and operations; (2) geometry and spatial sense; (3) measurement; (4) data analysis, statistics, and…

  13. Sample Curriculum Model, Grade 3, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 3 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  14. Sample Curriculum Model, Grade 4, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 4 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  15. Sample Curriculum Model, Grade K, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for Kindergarten mathematics based on the 1998 Arkansas State Mathematics Framework. The model is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra,…

  16. Sample Curriculum Model, Grade 5, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 5 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  17. Sample Curriculum Model, Grade 2, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 2 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  18. Sample Curriculum Model, Grade 7, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 7 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  19. Sample Curriculum Model, Grade 6, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 6 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  20. Sample Curriculum Model, Grade 8, Based on the 1998 Arkansas State Mathematics Framework.

    Science.gov (United States)

    Arkansas State Dept. of Education, Little Rock.

    This document consists of a sample curriculum model for grade 8 mathematics based on the 1998 Arkansas State Mathematics Framework. The document is divided into five sections: (1) Number Sense, Properties, and Operations; (2) Geometry and Spatial Sense; (3) Measurement; (4) Data Analysis, Statistics, and Probability; and (5) Patterns, Algebra, and…

  1. Impact of Spina Bifida on Parental Caregivers: Findings from a Survey of Arkansas Families

    Science.gov (United States)

    Grosse, Scott D.; Flores, Alina L.; Ouyang, Lijing; Robbins, James M.; Tilford, John M.

    2009-01-01

    The well-being of caregivers of children with spina bifida and other conditions is an important topic. We interviewed the primary caregivers of 98 children aged 0-17 years with spina bifida sampled from a population-based birth defects registry in Arkansas and the caregivers of 49 unaffected children. Measures of caregiver well-being were compared…

  2. Examination of the rice blast pathogen population diversity in Arkansas, USA – Stable or Unstable?

    Science.gov (United States)

    Over the past 17 years, isolates of Pyricularia oryzae (= P. grisea) have been recovered from commercial rice fields in Arkansas. Annual samples have typically included 100–500 isolates recovered from 5 to 10 cultivars from 10 different counties with the majority of the isolates being recovered from...

  3. Ready to Lead? A Study of Arkansas and Louisiana Charter School Principals' Leadership Skills

    Science.gov (United States)

    Price, Creshun Anjal

    2013-01-01

    The purpose of this study was to explore the relationship of Arkansas and Louisiana district conversion and open-enrollment charter school principals' background characteristics, leadership skills, and school success. A quantitative methodology was used to test the research questions prescribed in the study. Data was collected using a survey. The…

  4. Pharmaceuticals and other organic chemicals in selected north-central and northwestern Arkansas streams

    Science.gov (United States)

    Haggard, B.E.; Galloway, J.M.; Green, W.R.; Meyer, M.T.

    2006-01-01

    Recently, our attention has focused on the low level detection of many antibiotics, pharmaceuticals, and other organic chemicals in water resources. The limited studies available suggest that urban or rural streams receiving wastewater effluent are more susceptible to contamination. The purpose of this study was to evaluate the occurrence of antibiotics, pharmaceuticals, and other organic chemicals at 18 sites on seven selected streams in Arkansas, USA, during March, April, and August 2004. Water samples were collected upstream and downstream from the influence of effluent discharges in northwestern Arkansas and at one site on a relatively undeveloped stream in north-central Arkansas. At least one antibiotic, pharmaceutical, or other organic chemical was detected at all sites, except at Spavinaw Creek near Mayesville, Arkansas. The greatest number of detections was observed at Mud Creek downstream from an effluent discharge, including 31 pharmaceuticals and other organic chemicals. The detection of these chemicals occurred in higher frequency at sites downstream from effluent discharges compared to those sites upstream from effluent discharges; total chemical concentration was also greater downstream. Wastewater effluent discharge increased the concentrations of detergent metabolites, fire retardants, fragrances and flavors, and steroids in these streams. Antibiotics and associated degradation products were only found at two streams downstream from effluent discharges. Overall, 42 of the 108 chemicals targeted in this study were found in water samples from at least one site, and the most frequently detected organic chemicals included caffeine, phenol, para-cresol, and acetyl hexamethyl tetrahydro naphthalene (AHTN). ?? ASA, CSSA, SSSA.

  5. Teacher Pension Incentives, Retirement Behavior, and Potential for Reform in Arkansas. Conference Paper 2009-10

    Science.gov (United States)

    Costrell, Robert M.; McGee, Josh B.

    2009-01-01

    In this paper, we present an analysis of the Arkansas Teacher Retirement System (ATRS) pension plan and an empirical investigation of the behavioral response to that plan, as well as to a possible reform plan. We begin by describing the plan parameters and discussing the incentives these parameters create. We then estimate the effect of pension…

  6. Teacher Pension Incentives, Retirement Behavior, and Potential for Reform in Arkansas

    Science.gov (United States)

    Costrell, Robert M.; McGee, Josh B.

    2010-01-01

    The authors analyze the Arkansas teacher pension plan and empirically gauge the behavioral response to incentives embedded in that plan and to possible reforms. The pattern of pension wealth accrual creates sharp incentives to work until eligible for early or normal retirement, often in one's early fifties, and to separate shortly thereafter. We…

  7. Field evaluation of four spatial repellent devices against Arkansas rice-land mosquitoes

    Science.gov (United States)

    Four commercially available spatial repellent devices were tested in a rice land habitat near Stuttgart, Arkansas after semi-field level assessments had been made at the Center for Medical, Agricultural, and Veterinary Entomology, ARS, USDA in Gainesville, FL. OFF! Clip-On® (a.i. metofluthrin, S.C....

  8. Drought and deluge: Effects of recent climate variability on groundwater levels in eastern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Schrader, T.P.

    2013-01-01

    Arkansas experienced wide extremes in climate variability during the period of 2005 to 2010, recording the largest annual precipitation ever recorded in the State (100.05 inches) in 2009. Many weather stations across the State reported between 80 to 90 inches of rainfall in 2009. For comparison, the average annual precipitation in Little Rock, Arkansas, for the period 1878 to 2010 was 47.1 inches. In contrast, 2005 and 2010 were the 7th and 14th driest years on record in Little Rock with 34.55 and 36.52 inches, respectively; both tied as the hottest years ever recorded in Arkansas. The wettest year on record in Little Rock (2009) was interspersed within these dry years, with a total of 81.79 inches. Fifteen weather stations within the State ranked 2009 as the wettest year on record. Extremes in annual precipitation rates may lead to greater variability in groundwater recharge rates and water use, particularly in the agricultural areas in eastern Arkansas that rely heavily on groundwater produced from the Mississippi River Valley alluvial aquifer (hereafter referred to as the alluvial aquifer). How does this variability affect the groundwater system and water use therein? Are the effects of this variability discernable in measured water levels in wells? Czarnecki and Schrader examined these questions and provided some insights, the results of which are presented here.

  9. The Effect of Performance Pay in Little Rock, Arkansas on Student Achievement. Research Brief

    Science.gov (United States)

    Winters, Marcus; Greene, Jay; Ritter, Gary; Marsh, Ryan

    2008-01-01

    This paper examines evidence from a performance-pay program implemented in five Little Rock, Arkansas elementary schools between 2004 and 2007. Using a differences-in-differences approach, the evidence shows that students whose teachers were eligible for performance pay made substantially larger test score gains in math, reading, and language than…

  10. Child Care Needs of Mothers in Arkansas' Labor Force: A Statistical Study.

    Science.gov (United States)

    Arkansas Advocates for Children and Families, Little Rock.

    The major purpose of this paper is to increase employers' awareness of the impact that the rising number of working mothers has had on the need for day care in Arkansas as a whole and in their specific business locales. It is hoped that employers will be motivated to investigate and evaluate the advantages of providing some form of day care…

  11. Human Resources Promotes Diversity and Inclusiveness at the University of Arkansas

    Science.gov (United States)

    Taylor, Barbara

    2007-01-01

    Advancing diversity and inclusiveness on campus is certainly a top priority for many institutions today. The question often is not "Should we promote diversity in our campus community?" but rather "How do we go about doing it?" At the University of Arkansas, the chancellor had long declared that diversity was his first and most important goal.…

  12. 76 FR 36142 - Arkansas; Amendment No. 6 to Notice of a Major Disaster Declaration

    Science.gov (United States)

    2011-06-21

    ... Unemployment Assistance (DUA); 97.046, Fire Management Assistance Grant; 97.048, Disaster Housing Assistance to... Assistance--Disaster Housing Operations for Individuals and Households; 97.050, Presidentially Declared... SECURITY Federal Emergency Management Agency Arkansas; Amendment No. 6 to Notice of a Major...

  13. 76 FR 37360 - Arkansas; Amendment No. 7 to Notice of a Major Disaster Declaration

    Science.gov (United States)

    2011-06-27

    ..., Disaster Unemployment Assistance (DUA); 97.046, Fire Management Assistance Grant; 97.048, Disaster Housing... Declared Disaster Assistance--Disaster Housing Operations for Individuals and Households; 97.050... SECURITY Federal Emergency Management Agency Arkansas; Amendment No. 7 to Notice of a Major...

  14. Inhibition of enterovirus 71 (EV-71 infections by a novel antiviral peptide derived from EV-71 capsid protein VP1.

    Directory of Open Access Journals (Sweden)

    Chee Wah Tan

    Full Text Available Enterovirus 71 (EV-71 is the main causative agent of hand, foot and mouth disease (HFMD. In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC(50 values ranging from 6-9.3 µM in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71.

  15. Development of Novel Vaccines against Enterovirus-71

    OpenAIRE

    Pinn Tsin Isabel Yee; Chit Laa Poh

    2015-01-01

    The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71) and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and tr...

  16. Diseases caused by enterovirus 71 infection.

    Science.gov (United States)

    Lee, Ta-Chung; Guo, How-Ran; Su, Huey-Jen Jenny; Yang, Yi-Ching; Chang, Hsiao-Ling; Chen, Kow-Tong

    2009-10-01

    The purpose of this review was to explore the epidemiology, pathogenesis, virology, and management of enterovirus 71 (EV71) infection. Published literature was surveyed by Medline using the keyword "EV71." The reported incidence of cases of hand-foot-mouth disease/herpangina varied from year to year; seasonal variations in incidence were observed, with a peak in incidence during the summer season. Most cases of hand-foot-mouth disease/herpangina hospitalized for complications occurred in children less than 5 years old. The brainstem was the most likely major target of EV71 infection. Different enteroviruses cocirculate in the community annually. The emergence of the EV71 epidemic in the Asia Pacific region has been associated with the circulation of 5 genetic lineages (genotypes B3, B4, C1, C2, C4) that appear to be undergoing rapid evolutionary changes. The relationship between the gene structure of the EV71 virus and the factors that ensure its survival, ease of transmission, and evasion of immunity is still unclear. EV71 central nervous system involvement causes serious clinical illness, death, and long-term neurologic and psychiatric disorders in young children. EV71 infection has emerged as an important public health problem. Vaccine development is recommended for the prevention of EV71 infection in the future. PMID:20118685

  17. Unpublished Interim Digital Geologic Map of Hot Springs National Park and Vicinity, Arkansas (NPS, GRD, GRI, HOSP, HOSP digital map) adapted from the interim Arkansas Geological Survey DGM-HSR-003 by Johnson and Hanson (2011)

    Data.gov (United States)

    National Park Service, Department of the Interior — The Unpublished Interim Digital Geologic Map of Hot Springs National Park and Vicinity, Arkansas is composed of GIS data layers complete with ArcMap 9.3 layer...

  18. 31 CFR 7.1 - Purpose.

    Science.gov (United States)

    2010-07-01

    ..., 37 CFR part 100. The purpose of this part 7 is to implement for the Treasury Department the foregoing... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Purpose. 7.1 Section 7.1 Money and... nonexclusive, irrevocable, royalty-free license in the invention, and the procedures for the determination...

  19. Outbreak of Enterovirus - 71 Meningitis in Calicut

    Directory of Open Access Journals (Sweden)

    CK Sasidharan

    2016-04-01

    Full Text Available Objective: Enterovirus 71(EV 71 causes wide spectrum of infections ranging from asymptomatic conditions to clinical syndromes like diarrhea, rash, hand-foot-and mouth disease (HFMD, herpangina, aseptic meningitis, encephalitis, myocarditis, acute flaccid paralysis, bulbar and brainstem encephalitis Guillain Barre syndrome, pulmonary haemorrhage. This study deals with an outbreak of aseptic meningitis in children caused by EV 71 virus. Methods: The authors report an outbreak of aseptic meningitis in children in and around Calicut in June 2008. Clinical and laboratory study was done in collaboration with National Centre for Disease Control, New Delhi. 149 children with aseptic meningitis were studied and followed up from June 2008 to May 2009. Result: All children had clinical features suggestive of aseptic meningitis and serology showed the rising antibody titre against EV 71 virus infection. CSF analysis also showed four fold rise in antibodies in one and ≥ 1:2 neutralising antibodies titer against EV- 71 in four samples indicating meningitis due to EV-71. Conclusion: EV 71 was identified as the causative agent of the outbreak of aseptic meningitis in the study and the fact that the EV 71 infection has evolved from minor illness like HFMD to major illness like aseptic meningitis from the same locality is truly alarming.

  20. 41 CFR 105-71.132 - Equipment.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Equipment. 105-71.132...-Award Requirements/Changes, Property, and Subawards § 105-71.132 Equipment. (a) Title. Subject to the obligations and conditions set forth in this section, title to equipment acquired under a grant or...

  1. 28 CFR 71.42 - Judicial review.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Judicial review. 71.42 Section 71.42....42 Judicial review. Section 3805 of title 31, United States Code, authorizes judicial review by an... assessments under this part and specifies the procedures for such review....

  2. 10 CFR 71.113 - Document control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Document control. 71.113 Section 71.113 Energy NUCLEAR....113 Document control. The licensee, certificate holder, and applicant for a CoC shall establish measures to control the issuance of documents such as instructions, procedures, and drawings,...

  3. 36 CFR 72.71 - Information collection.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Information collection. 72.71... PARK AND RECREATION RECOVERY ACT OF 1978 Post-Completion Compliance Responsibilities § 72.71 Information collection. The information collection requirements contained in § 72.72 have been approved by...

  4. 48 CFR 1516.303-71 - Definition.

    Science.gov (United States)

    2010-10-01

    ... METHODS AND CONTRACT TYPES TYPES OF CONTRACTS Cost-Reimbursement Contracts 1516.303-71 Definition. Cost... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Definition. 1516.303-71... contractor for all allowable costs necessary to accomplish the project under the contract. This...

  5. 28 CFR 540.71 - Procedures.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Procedures. 540.71 Section 540.71 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT CONTACT WITH... inmate may receive hardcover publications and newspaper only from the publisher, from a book club,...

  6. 24 CFR 511.71 - Administrative costs.

    Science.gov (United States)

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Administrative costs. 511.71... Administration § 511.71 Administrative costs. (a) Maximum amount. Any grantee may use not to exceed 10 percent of... years for administrative costs eligible under paragraphs (b) and (c) of this section. Eligible...

  7. 45 CFR 1336.71 - Administrative costs.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Administrative costs. 1336.71 Section 1336.71... Administrative costs. Reasonable administrative costs of the RLF may be paid out of the loan fund. The grant award agreement between the Loan Administrator and ANA will set forth the allowable administrative...

  8. 10 CFR 71.133 - Corrective action.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Corrective action. 71.133 Section 71.133 Energy NUCLEAR....133 Corrective action. The licensee, certificate holder, and applicant for a CoC shall establish... determined and corrective action taken to preclude repetition. The identification of the...

  9. 40 CFR 406.71 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... in 40 CFR part 401 shall apply to this subpart. ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 406.71 Section 406.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES...

  10. 41 CFR 105-71.134 - Copyrights.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Copyrights. 105-71.134...-Award Requirements/Changes, Property, and Subawards § 105-71.134 Copyrights. The Federal awarding agency... to authorize others to use, for Federal Government purposes: (a) The copyright in any work...

  11. Challenges to licensure of enterovirus 71 vaccines.

    Directory of Open Access Journals (Sweden)

    Min-Shi Lee

    Full Text Available Human enteroviruses usually cause self-limited infections except polioviruses and enterovirus 71 (EV71, which frequently involve neurological complications. EV71 vaccines are being evaluated in humans. However, several challenges to licensure of EV71 vaccines need to be addressed. Firstly, EV71 and coxsackievirus A (CA are frequently found to co-circulate and cause hand-foot-mouth disease (HFMD. A polyvalent vaccine that can provide protection against EV71 and prevalent CA are desirable. Secondly, infants are the target population of HFMD vaccines and it would need multi-national efficacy trials to prove clinical protection and speed up the licensure and usage of HFMD vaccines in children. An international network for enterovirus surveillance and clinical trials is urgently needed. Thirdly, EV71 is found to evolve quickly in the past 15 years. Prospective cohort studies are warranted to clarify clinical and epidemiological significances of the antigenic and genetic variations between different EV71 genogroups, which is critical for vaccine design.

  12. 49 CFR 397.71 - Federal standards.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Federal standards. 397.71 Section 397.71... standards. (a) A State or Indian tribe shall comply with the Federal standards under paragraph (b) of this... or may not be transported. (b) The Federal standards are as follows: (1) Enhancement of public...

  13. 44 CFR 71.4 - Documentation.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Documentation. 71.4 Section... LEGISLATION § 71.4 Documentation. (a) In order to obtain a new policy of flood insurance for a structure which... protected area,” the owner of the structure must submit the documentation described in this section in...

  14. 40 CFR 436.71 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 436.71 Section 436.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS MINERAL MINING AND PROCESSING POINT SOURCE CATEGORY Asbestos and...

  15. 34 CFR 200.71 - LEA eligibility.

    Science.gov (United States)

    2010-07-01

    ... least five percent of the LEA's total population ages 5 to 17 years, inclusive. (d) Education finance... 34 Education 1 2010-07-01 2010-07-01 false LEA eligibility. 200.71 Section 200.71 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY...

  16. 40 CFR 405.71 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 405.71 Section 405.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen...

  17. 10 CFR 7.1 - Policy.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Policy. 7.1 Section 7.1 Energy NUCLEAR REGULATORY... committees shall be balanced in their membership in terms of the points of view represented and the functions to be performed. (g) The Congress shall be kept informed of the number, purpose,...

  18. 21 CFR 640.71 - Manufacturing responsibility.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Manufacturing responsibility. 640.71 Section 640...) BIOLOGICS ADDITIONAL STANDARDS FOR HUMAN BLOOD AND BLOOD PRODUCTS Source Plasma § 640.71 Manufacturing responsibility. (a) All steps in the manufacturing of Source Plasma, including donor examination,...

  19. 31 CFR 10.71 - Discovery.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Discovery. 10.71 Section 10.71 Money and Finance: Treasury Office of the Secretary of the Treasury PRACTICE BEFORE THE INTERNAL REVENUE... law; (7) The material sought relates to mental impressions, conclusions, or legal theories of...

  20. Production of EV71 vaccine candidates.

    Science.gov (United States)

    Chong, Pele; Hsieh, Shih-Yang; Liu, Chia-Chyi; Chou, Ai-Hsiang; Chang, Jui-Yuan; Wu, Suh-Chin; Liu, Shih-Jen; Chow, Yen-Hung; Su, Ih-Jen; Klein, Michel

    2012-12-01

    Enterovirus 71 (EV71) is now recognized as an emerging neurotropic virus in Asia and with Coxsackie virus (CV) it is the other major causative agent of hand-foot-mouth diseases (HFMD). Effective medications and/or prophylactic vaccines against HFMD are urgently needed. From a scientific (the feasibility of bioprocess, immunological responses and potency in animal challenge model) and business development (cost of goods) points of view, we in this review address and discuss the pros and cons of different EV71 vaccine candidates that have been produced and evaluated in animal models. Epitope-based synthetic peptide vaccine candidates containing residues 211-225 of VP1 formulated with Freund's adjuvant (CFA/IFA) elicited low EV71 virus neutralizing antibody responses, but were protective in the suckling mouse challenge model. Among recombinant EV71 subunits (rVP1, rVP2 and rVP3) expressed in E. coli, purified and formulated with CFA/IFA, only VP1 elicited mouse antibody responses with measurable EV71-specific virus neutralization titers. Immunization of mice with either a DNA plasmid containing VP1 gene or VP1 expressed in Salmonella typhimurium also generated neutralizing antibody responses and protected animals against a live EV71 challenge. Recombinant EV71 virus-like particles (rVLP) produced from baculovirus formulated either with CFA/IFA or alum elicited good virus neutralization titers in both mice and non-human primates, and were found to be protective in the suckling mouse EV71 challenge model. Synthetic peptides or recombinant EV71 subunit vaccines (rVP1 and rVLP) formulated in alum were found to be poorly immunogenic in rabbits. Only formalin-inactivated (FI) EV71 virions formulated in alum elicited cross-neutralizing antibodies against different EV71 genotypes in mice, rabbits and non-human primates but induced weak neutralizing responses against CAV16. From a regulatory, economic and market acceptability standpoint, FI-EV71 virion vaccines are the most

  1. Arkansas: a leading laboratory for health care payment and delivery system reform.

    Science.gov (United States)

    Bachrach, Deborah; du Pont, Lammot; Lipson, Mindy

    2014-08-01

    As states' Medicaid programs continue to evolve from traditional fee-for-service to value-based health care delivery, there is growing recognition that systemwide multipayer approaches provide the market power needed to address the triple aim of improved patient care, improved health of populations, and reduced costs. Federal initiatives, such as the State Innovation Model grant program, make significant funds available for states seeking to transform their health care systems. In crafting their reform strategies, states can learn from early innovators. This issue brief focuses on one such state: Arkansas. Insights and lessons from the Arkansas Health Care Payment Improvement Initiative (AHCPII) suggest that progress is best gained through an inclusive, deliberative process facilitated by committed leadership, a shared agreement on root problems and opportunities for improvement, and a strategy grounded in the state's particular health care landscape. PMID:25204031

  2. Arkansas: a leading laboratory for health care payment and delivery system reform.

    Science.gov (United States)

    Bachrach, Deborah; du Pont, Lammot; Lipson, Mindy

    2014-08-01

    As states' Medicaid programs continue to evolve from traditional fee-for-service to value-based health care delivery, there is growing recognition that systemwide multipayer approaches provide the market power needed to address the triple aim of improved patient care, improved health of populations, and reduced costs. Federal initiatives, such as the State Innovation Model grant program, make significant funds available for states seeking to transform their health care systems. In crafting their reform strategies, states can learn from early innovators. This issue brief focuses on one such state: Arkansas. Insights and lessons from the Arkansas Health Care Payment Improvement Initiative (AHCPII) suggest that progress is best gained through an inclusive, deliberative process facilitated by committed leadership, a shared agreement on root problems and opportunities for improvement, and a strategy grounded in the state's particular health care landscape.

  3. Building Partnerships With Rural Arkansas Faith Communities to Promote Veterans’ Mental Health: Lessons Learned

    Science.gov (United States)

    Sullivan, Greer; Hunt, Justin; Haynes, Tiffany F.; Bryant, Keneshia; Cheney, Ann M.; Pyne, Jeffrey M.; Reaves, Christina; Sullivan, Steve; Lewis, Caleb; Barnes, Bonita; Barnes, Michael; Hudson, Cliff; Jegley, Susan; Larkin, Bridgette; Russell, Shane; White, Penny; Gilmore, LaNissa; Claypoole, Sterling; Smith, Rev. Johnny; Richison, Ruth

    2014-01-01

    Background The Mental Health–Clergy Partnership Program established partnerships between institutional (Department of Veterans’ Affairs [VA] chaplains, mental health providers) and community (local clergy, parishioners) groups to develop programs to assist rural veterans with mental health needs. Objectives Describe the development, challenges, and lessons learned from the Mental Health–Clergy Partnership Program in three Arkansas towns between 2009 and 2012. Methods Researchers identified three rural Arkansas sites, established local advisory boards, and obtained quantitative ratings of the extent to which partnerships were participatory. Results Partnerships seemed to become more participatory over time. Each site developed distinctive programs with variation in fidelity to original program goals. Challenges included developing trust and maintaining racial diversity in local program leadership. Conclusions Academics can partner with local faith communities to create unique programs that benefit the mental health of returning veterans. Research is needed to determine the effectiveness of community based programs, especially relative to typical “top-down” outreach approaches. PMID:24859098

  4. 40 CFR 98.71 - Reporting threshold.

    Science.gov (United States)

    2010-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Ammonia Manufacturing § 98.71 Reporting threshold. You must report GHG emissions under this subpart if your facility contains an ammonia manufacturing process and the...

  5. 28 CFR 71.34 - Evidence.

    Science.gov (United States)

    2010-07-01

    ... REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71.34 Evidence. (a... outweighed by the danger of unfair prejudice, confusion of the issues, or by considerations of undue delay...

  6. 40 CFR 279.71 - Prohibitions.

    Science.gov (United States)

    2010-07-01

    ... THE MANAGEMENT OF USED OIL Standards for Used Oil Fuel Marketers § 279.71 Prohibitions. A used oil fuel marketer may initiate a shipment of off-specification used oil only to a used oil burner who:...

  7. 40 CFR 71.9 - Permit fees.

    Science.gov (United States)

    2010-07-01

    ... the Consumer Price Index. (2) Part 71 permit program costs and fees will be reviewed by the... Administrator, the changes will be published in the Federal Register. (o) Use of revenue. All fees,...

  8. Crystal Structure of Human Enterovirus 71

    Energy Technology Data Exchange (ETDEWEB)

    Plevka, Pavel; Perera, Rushika; Cardosa, Jane; Kuhn, Richard J.; Rossmann, Michael G. (Purdue); (Sentinext)

    2013-04-08

    Enterovirus 71 is a picornavirus associated with fatal neurological illness in infants and young children. Here, we report the crystal structure of enterovirus 71 and show that, unlike in other enteroviruses, the 'pocket factor,' a small molecule that stabilizes the virus, is partly exposed on the floor of the 'canyon.' Thus, the structure of antiviral compounds may require a hydrophilic head group designed to interact with residues at the entrance of the pocket.

  9. A new species of Sellaphora (Sellaphoraceae) from Hannaberry Lake, Arkansas, U.S.A.

    OpenAIRE

    Enache, Mihaela D.; Potapova, Marina

    2009-01-01

    A new small-size species of Sellaphora was found in sediments from Hannaberry Lake, Arkansas, during the National Lakes Assessment project conducted by the United States Environmental Protection Agency. The species was studied with light microscopy and scanning electron microscopy. It differs from previously reported Sellaphora species by its small and delicate frustule with striation irresolvable in light microscopy. Here we present details on its morphology and size variation and report the...

  10. Arkansas and Louisiana Aeromagnetic and Gravity Maps and Data - A Website for Distribution of Data

    Science.gov (United States)

    Bankey, Viki; Daniels, David L.

    2008-01-01

    This report contains digital data, image files, and text files describing data formats for aeromagnetic and gravity data used to compile the State aeromagnetic and gravity maps of Arkansas and Louisiana. The digital files include grids, images, ArcInfo, and Geosoft compatible files. In some of the data folders, ASCII files with the extension 'txt' describe the format and contents of the data files. Read the 'txt' files before using the data files.

  11. Molecular Subtype Analyses of Campylobacter spp. from Arkansas and California Poultry Operations

    OpenAIRE

    Hiett, K. L; Stern, N. J.; Fedorka-Cray, P.; Cox, N. A.; Musgrove, M. T.; Ladely, S.

    2002-01-01

    Campylobacter isolates from diverse samples within broiler production and processing environments were typed by using flaA short variable region DNA sequence analysis. Sixteen flocks from four different farms representing two broiler producers in Arkansas and California were analyzed. Fourteen of the flocks (87.5%) were Campylobacter-positive; two remained negative throughout the 6-week rearing period. In general, multiple clones were present within a flock. Additionally, clones found within ...

  12. Atmospheric Contributors to Heavy Rainfall Events in the Arkansas-Red River Basin

    OpenAIRE

    McCorkle, Taylor A.; Skylar S. Williams; Pfeiffer, Timothy A.; Basara, Jeffrey B.

    2016-01-01

    This study analyzed the top 1% 24-hour rainfall events from 1994 to 2013 at eight climatological sites that represent the east to west precipitation gradient across the Arkansas-Red River Basin in North America. A total of 131 cases were identified and subsequently classified on the synoptic-scale, mesoscale, and local-scale to compile a climatological analysis of these extreme, heavy rainfall events based on atmospheric forcings. For each location, the prominent midtropospheric pattern, meso...

  13. Molecular Detection of Rickettsia Species Within Ticks (Acari: Ixodidae) Collected from Arkansas United States.

    Science.gov (United States)

    Trout Fryxell, R T; Steelman, C D; Szalanski, A L; Billingsley, P M; Williamson, P C

    2015-05-01

    Rocky Mountain spotted fever (RMSF), caused by the etiological agent Rickettsia rickettsii, is the most severe and frequently reported rickettsial illness in the United States, and is commonly diagnosed throughout the southeast. With the discoveries of Rickettsia parkeri and other spotted fever group rickettsiae (SFGR) in ticks, it remains inconclusive if the cases reported as RMSF are truly caused by R. rickettsii or other SFGR. Arkansas reports one of the highest incidence rates of RMSF in the country; consequently, to identify the rickettsiae in Arkansas, 1,731 ticks, 250 white-tailed deer, and 189 canines were screened by polymerase chain reaction (PCR) for the rickettsial genes gltA, rompB, and ompA. None of the white-tailed deer were positive, while two of the canines (1.1%) and 502 (29.0%) of the ticks were PCR positive. Five different tick species were PCR positive: 244 (37%) Amblyomma americanum L., 130 (38%) Ixodes scapularis Say, 65 (39%) Amblyomma maculatum (Koch), 30 (9%) Rhipicephalus sanguineus Latreille, 7 (4%) Dermacentor variabilis Say, and 26 (44%) unidentified Amblyomma ticks. None of the sequenced products were homologous to R. rickettsii. The most common Rickettsia via rompB amplification was Rickettsia montanensis and nonpathogenic Candidatus Rickettsia amblyommii, whereas with ompA amplification the most common Rickettsia was Ca. R. amblyommii. Many tick specimens collected in northwest Arkansas were PCR positive and these were commonly A. americanum harboring Ca. R. amblyommii, a currently nonpathogenic Rickettsia. Data reported here indicate that pathogenic R. rickettsii was absent from these ticks and suggest by extension that other SFGR are likely the causative agents for Arkansas diagnosed RMSF cases.

  14. Arthropods of Steel Creek, Buffalo National River, Arkansas. III. Heteroptera (Insecta: Hemiptera)

    OpenAIRE

    Skvarla, Michael Joseph; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the third in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and B...

  15. Terrestrial arthropods of Steel Creek, Buffalo National River, Arkansas. II. Sawflies (Insecta: Hymenoptera: " Symphyta ")

    OpenAIRE

    Skvarla, Michael Joseph; Smith, David R.; Fisher, Danielle M.; Dowling, Ashley P.G.

    2016-01-01

    Abstract Background This is the second in a series of papers detailing the terrestrial arthropods collected during an intensive survey of a site near Steel Creek campground along the Buffalo National River in Arkansas. The survey was conducted over a period of eight and a half months using twelve trap types – Malaise traps, canopy traps (upper and lower collector), Lindgren multifunnel traps (black, green, and purple), pan traps (blue, purple, red, white, and yellow), and pitfall traps – and ...

  16. Development of Novel Vaccines against Enterovirus-71.

    Science.gov (United States)

    Yee, Pinn Tsin Isabel; Poh, Chit Laa

    2016-01-01

    The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71) and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and treatment agents against EV-A71 to prevent further fatalities. Research groups have developed experimental inactivated vaccines, recombinant Viral Protein 1 (VP1) vaccine and virus-like particles (VLPs). The inactivated EV-A71 vaccine is considered the safest viral vaccine, as there will be no reversion to the infectious wild type strain. The recombinant VP1 vaccine is a cost-effective immunogen, while VLPs contain an arrangement of epitopes that can elicit neutralizing antibodies against the virus. As each type of vaccine has its advantages and disadvantages, increased studies are required in the development of such vaccines, whereby high efficacy, long-lasting immunity, minimal risk to those vaccinated, safe and easy production, low cost, dispensing the need for refrigeration and convenient delivery are the major goals in their design. PMID:26729152

  17. Development of Novel Vaccines against Enterovirus-71

    Directory of Open Access Journals (Sweden)

    Pinn Tsin Isabel Yee

    2015-12-01

    Full Text Available The hand, foot and mouth disease is caused by a group of Enteroviruses such as Enterovirus 71 (EV-A71 and Coxsackievirus CV-A5, CV-A8, and CV-A16. Mild symptoms of EV-A71 infection in children range from high fever, vomiting, rashes and ulcers in mouth but can produce more severe symptoms such as brainstem and cerebellar encephalitis, leading up to cardiopulmonary failure and death. The lack of vaccines and antiviral drugs against EV-A71 highlights the urgency of developing preventive and treatment agents against EV-A71 to prevent further fatalities. Research groups have developed experimental inactivated vaccines, recombinant Viral Protein 1 (VP1 vaccine and virus-like particles (VLPs. The inactivated EV-A71 vaccine is considered the safest viral vaccine, as there will be no reversion to the infectious wild type strain. The recombinant VP1 vaccine is a cost-effective immunogen, while VLPs contain an arrangement of epitopes that can elicit neutralizing antibodies against the virus. As each type of vaccine has its advantages and disadvantages, increased studies are required in the development of such vaccines, whereby high efficacy, long-lasting immunity, minimal risk to those vaccinated, safe and easy production, low cost, dispensing the need for refrigeration and convenient delivery are the major goals in their design.

  18. Both ERK1 and ERK2 are required for enterovirus 71 (EV71) efficient replication.

    Science.gov (United States)

    Zhu, Meng; Duan, Hao; Gao, Meng; Zhang, Hao; Peng, Yihong

    2015-03-01

    It has been demonstrated that MEK1, one of the two MEK isoforms in Raf-MEK-ERK1/2 pathway, is essential for successful EV71 propagation. However, the distinct function of ERK1 and ERK2 isoforms, the downstream kinases of MEKs, remains unclear in EV71 replication. In this study, specific ERK siRNAs and selective inhibitor U0126 were applied. Silencing specific ERK did not significantly impact on the EV71-caused biphasic activation of the other ERK isoform, suggesting the EV71-induced activations of ERK1 and ERK2 were non-discriminative and independent to one another. Knockdown of either ERK1 or ERK2 markedly impaired progeny EV71 propagation (both by more than 90%), progeny viral RNA amplification (either by about 30% to 40%) and protein synthesis (both by around 70%), indicating both ERK1 and ERK2 were critical and not interchangeable to EV71 propagation. Moreover, suppression of EV71 replication by inhibiting both early and late phases of ERK1/2 activation showed no significant difference from that of only blocking the late phase, supporting the late phase activation was more importantly responsible for EV71 life cycle. Taken together, this study for the first time identified both ERK1 and ERK2 were required for EV71 efficient replication and further verified the important role of MEK1-ERK1/2 in EV71 replication.

  19. MicroRNA-23b inhibits enterovirus 71 replication through downregulation of EV71 VPl protein.

    Science.gov (United States)

    Wen, Bai-ping; Dai, Hong-jian; Yang, Yue-huang; Zhuang, Yu; Sheng, Ru

    2013-01-01

    Enterovirus 71 (EV71) is one of the causative pathogens of hand-foot-and-mouth disease and effective antiviral agents and vaccines against this virus have, to date, not been available. MicroRNAs (miRNAs) are a recently discovered class of RNAs with the function of post-transcriptional gene expression regulation. It has been demonstrated that miRNAs play important roles in the complicated interaction network between virus and host, while few studies have explored the role of miRNAs in EV71 infection. A recent study showed that hsa-miR-23b was downregulated significantly in cell-infected viruses. To address this issue, biological software miRanda was first used to predict possible target sites of miR-23b at EV71 gene sequence, then to confirm it by luciferase assay. miR-23b mimics were transfected to verify its effects on infection of EV71. These results suggest that miR-23b and upregulation of miR-23b inhibited the replication of EV71 by targeting at EV71 3'UTR conserved sequence. Taken together, miR-23b could inhibit EV71 replication through downregulation of EV71 VPl protein. These results may enhance our understanding on the prevention and treatment of hand-foot-and-mouth disease caused by EV71 infection.

  20. Both ERK1 and ERK2 Are Required for Enterovirus 71 (EV71 Efficient Replication

    Directory of Open Access Journals (Sweden)

    Meng Zhu

    2015-03-01

    Full Text Available It has been demonstrated that MEK1, one of the two MEK isoforms in Raf-MEK-ERK1/2 pathway, is essential for successful EV71 propagation. However, the distinct function of ERK1 and ERK2 isoforms, the downstream kinases of MEKs, remains unclear in EV71 replication. In this study, specific ERK siRNAs and selective inhibitor U0126 were applied. Silencing specific ERK did not significantly impact on the EV71-caused biphasic activation of the other ERK isoform, suggesting the EV71-induced activations of ERK1 and ERK2 were non-discriminative and independent to one another. Knockdown of either ERK1 or ERK2 markedly impaired progeny EV71 propagation (both by more than 90%, progeny viral RNA amplification (either by about 30% to 40% and protein synthesis (both by around 70%, indicating both ERK1 and ERK2 were critical and not interchangeable to EV71 propagation. Moreover, suppression of EV71 replication by inhibiting both early and late phases of ERK1/2 activation showed no significant difference from that of only blocking the late phase, supporting the late phase activation was more importantly responsible for EV71 life cycle. Taken together, this study for the first time identified both ERK1 and ERK2 were required for EV71 efficient replication and further verified the important role of MEK1-ERK1/2 in EV71 replication.

  1. Methods and applications of electrical simulation in ground-water studies in the lower Arkansas and Verdigris River Valleys, Arkansas and Oklahoma

    Science.gov (United States)

    Bedinger, M.S.; Reed, J.E.; Wells, C.J.; Swafford, B.F.

    1970-01-01

    The Arkansas River Multiple-Purpose Plan will provide year-round navigation on the Arkansas River from near its mouth to Muskogee, Okla., and on the Verdigris River from Muskogee to Catoosa, Okla. The altered regimen in the Arkansas and Verdigris Rivers will affect ground-water conditions in the adjacent alluvial aquifers. In 1957 the U.S. Geological Survey and U.S. Army Corps of Engineers entered into a cooperative agreement for a comprehensive ground-water study of the lower Arkansas and Verdigris River valleys. At the request of the Corps of Engineers, the Geological Survey agreed to provide (1) basic ground-water data before, during, and after construction of the Multiple-Purpose Plan and (2) interpretation and projections of postconstruction ground-water conditions. The data collected were used by the Corps of Engineers in preliminary foundation and excavation estimates and by the Geological Survey as the basis for defining the hydrologic properties of, and the ground-water conditions in, the aquifer. The projections of postconstruction ground-water conditions were used by the Corps of Engineers in the planning, design, construction, and operation of the Multiple-Purpose Plan. Analysis and projections of ground-water conditions were made by use of electrical analog models. These models use the analogy between the flow of electricity in a resistance-capacitance circuit and the flow of a liquid in a porous and permeable medium. Verification provides a test of the validity of the analog to perform as the aquifer would, within the range of historic forces. The verification process consists of simulating the action of historic forces which have acted upon the aquifer and of duplicating the aquifer response with the analog. The areal distribution of accretion can be treated as an unknown and can be determined by analog simulation of the piezometric surface in an aquifer. Comparison of accretion with depth to piezometric surface below land surface shows that

  2. COPI is required for enterovirus 71 replication.

    Directory of Open Access Journals (Sweden)

    Jianmin Wang

    Full Text Available Enterovirus 71 (EV71, a member of the Picornaviridae family, is found in Asian countries where it causes a wide range of human diseases. No effective therapy is available for the treatment of these infections. Picornaviruses undergo RNA replication in association with membranes of infected cells. COPI and COPII have been shown to be involved in the formation of picornavirus-induced vesicles. Replication of several picornaviruses, including poliovirus and Echovirus 11 (EV11, is dependent on COPI or COPII. Here, we report that COPI, but not COPII, is required for EV71 replication. Replication of EV71 was inhibited by brefeldin A and golgicide A, inhibitors of COPI activity. Furthermore, we found EV71 2C protein interacted with COPI subunits by co-immunoprecipitation and GST pull-down assay, indicating that COPI coatomer might be directed to the viral replication complex through viral 2C protein. Additionally, because the pathway is conserved among different species of enteroviruses, it may represent a novel target for antiviral therapies.

  3. EV71 vaccine, an invaluable gift for children.

    Science.gov (United States)

    Liang, Zhenglun; Wang, Junzhi

    2014-10-01

    Enterovirus 71 (EV71) is a major pathogen for severe hand, foot and mouth disease (HFMD). Development of vaccines against EV71 would be the most effective approach to prevent the EV71 outbreak. Research and development (R&D) of EV71 vaccine was carried out in several Asian countries. Currently three companies in mainland China have completed Phase III clinical trials of inactivated EV71 whole-virus vaccines, whereas the other two companies have completed Phase I clinical trials separately in Taiwan and in Singapore. Results from those clinical trials have indicated high safety and immunogenicity of EV71 vaccine. Protective efficacies were over 90% on EV71-associated HFMD and over 80% on other EV71-associated diseases. In this paper, we summarize the results from three EV71 vaccine Phase III clinical trials and discuss the challenges of incorporating EV71 vaccine into Expanded Program on Immunization (EPI) in countries with EV71 epidemics.

  4. Protective effect of enterovirus-71 (EV71) virus-like particle vaccine against lethal EV71 infection in a neonatal mouse model

    OpenAIRE

    Cao, Lei; MAO, FENGFENG; Pang, Zheng; Yi, Yao; Qiu, Feng; Tian, Ruiguang; MENG, QINGLING; Jia, Zhiyuan; Bi, Shengli

    2015-01-01

    Enterovirus-71 (EV71) is a viral pathogen that causes severe cases of hand, foot and mouth disease (HFMD) among young children, with significant mortality. Effective vaccines against HFMD are urgently required. Several EV71 virus-like particle (VLP) vaccine candidates were found to be protective in the neonatal mouse EV71 challenge model. However, to what extent the VLP vaccine protects susceptible organs against EV71 infection in vivo has remained elusive. In the present study, the comprehen...

  5. Notes from the Field: Cluster of Tuberculosis Cases Among Marshallese Persons Residing in Arkansas - 2014-2015.

    Science.gov (United States)

    Rothfeldt, Laura Lester; Patil, Naveen; Haselow, Dirk T; Williams, Sandy Hainline; Wheeler, J Gary; Mukasa, Leonard N

    2016-01-01

    During early September 2014, the Arkansas Department of Health identified an increased number of tuberculosis (TB) cases among a unique population in a well-circumscribed geographical area in northwest Arkansas. The Compact of Free Association Act of 1985 (Public Law 99-239, amended in 2003 by Public Law 108-188) established the Republic of the Marshall Islands (RMI) as an independent nation, and persons from the RMI can travel freely (with valid RMI passport) to and from the United States as nonimmigrants without visas (1). Marshallese started settling in northwest Arkansas during the early 1990s because of employment and educational opportunities (2). According to the 2010 Census, an estimated 4,300 Marshallese resided in Arkansas (2), mostly within one county which ranked 6th in the United States for counties with the highest percentage of Native Hawaiians and Other Pacific Islanders (3). It is estimated that this number has been growing steadily each year since the 2010 Census; however, obtaining an accurate count is difficult. The RMI is a TB high-incidence country, with a case-rate of 212.7 per 100,000 persons for 2014, whereas the case-rate was 3.1 per 100,000 persons in Arkansas and 2.9 per 100,000 persons in the United States (4,5). Screening for either active TB or latent TB infection (LTBI) is not required for Marshallese entry to the United States (1). PMID:27560201

  6. The Natural Divisions of Arkansas: A Three Week Unit. Classroom Guide. Recommended for High School Level Social Studies, Earth Science and Biological Science Classes.

    Science.gov (United States)

    Foti, Thomas L.

    This unit is designed to (1) acquaint the student with Arkansas, (2) provide information on what an environment (natural system) is, and (3) provide information on how people relate to the environment as a whole. Natural systems and natural divisions (geographical areas) of Arkansas are described. Included in several sections are questions to…

  7. "There Were High Hopes and High Projections:" Examining the Social Construction of Target Populations in the Policy Design of the Arkansas Lottery Legislation

    Science.gov (United States)

    Copeland, Kristopher

    2013-01-01

    Lottery policies have been created by many states to generate additional funds to support public initiatives, such as higher education scholarships. In 2009, Arkansas adopted a lottery to generate higher education scholarships. The purpose of this qualitative case study was to examine the Arkansas state lottery policy design process to better…

  8. MRI of Enterovirus 71 myelitis with monoplegia

    Energy Technology Data Exchange (ETDEWEB)

    Shen, W.C. [Department of Radiology, and School of Medicine, China Medical College, China Medical College Hospital, Taiwan (Taiwan); Tsai, C.H. [Department of Paediatrics, and School of Medicine, China Medical College, China Medical College Hospital, Taiwan (Taiwan); Chiu, H.H. [Department of Paediatrics, China Medical College Hospital, No. 2 Yuh-Der Road, 407 Taichung, Taiwan (Taiwan); Chow, K.C. [Department of Medical Research, China Medical College Hospital, Taichung, Taiwan (Taiwan)

    2000-02-01

    We report two boys diagnosed as having herpangina and hand-foot-mouth disease complicated by monoplegia during the outbreak enterovirus infection in Taiwan in 1998. Enterovirus 71 was identified in the stool and throat swab; neither polio nor Coxsackie viruses was identified. MRI showed unilateral lesions in the anterior horns of the spinal cord at T11-12 and C2-5. Although the MRI findings and sites of these lesions were similar to those of poliovirus-associated poliomyelitis, the virological data indicated that these boys were infected with enterovirus type 71. (orig.)

  9. 肠道病毒EV71

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    肠道病毒EV71感染疾病是由肠道病毒EV71引起的传染病,多发生于婴幼儿,尤以3岁以下年龄组发病率最高,可引起手、足、口腔等部位的疱疹,个别患者可引起心肌炎、肺水肿、无菌性脑膜脑炎等并发症。

  10. A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema.

    Science.gov (United States)

    Victorio, Carla Bianca Luena; Xu, Yishi; Ng, Qimei; Chua, Beng Hooi; Alonso, Sylvie; Chow, Vincent T K; Chua, Kaw Bing

    2016-01-01

    Enterovirus 71 (EV-A71) is a neurotropic virus that sporadically causes fatal neurologic illness among infected children. Animal models of EV-A71 infection exist, but they do not recapitulate in animals the spectrum of disease and pathology observed in fatal human cases. Specifically, neurogenic pulmonary oedema (NPE)-the main cause of EV-A71 infection-related mortality-is not observed in any of these models. This limits their utility in understanding viral pathogenesis of neurologic infections. We report the development of a mouse model of EV-A71 infection displaying NPE in severely affected animals. We inoculated one-week-old BALB/c mice with an adapted EV-A71 strain and identified clinical signs consistent with observations in human cases and other animal models. We also observed respiratory distress in some mice. At necropsy, we found their lungs to be heavier and incompletely collapsed compared to other mice. Serum levels of catecholamines and histopathology of lung and brain tissues of these mice strongly indicated onset of NPE. The localization of virally-induced brain lesions also suggested a potential pathogenic mechanism for EV-A71-induced NPE. This novel mouse model of virally-induced NPE represents a valuable resource for studying viral mechanisms of neuro-pathogenesis and pre-clinical testing of potential therapeutics and prophylactics against EV-A71-related neurologic complications. PMID:27357918

  11. 48 CFR 246.408-71 - Aircraft.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Aircraft. 246.408-71... Aircraft. (a) The Federal Aviation Administration (FAA) has certain responsibilities and prerogatives in connection with some commercial aircraft and of aircraft equipment and accessories (Pub. L. 85-726 (72...

  12. 41 CFR 105-71.102 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... (Continued) GENERAL SERVICES ADMINISTRATION Regional Offices-General Services Administration 71-UNIFORM... equipment means the net invoice unit price of the property including the cost of modifications, attachments... under the United States Housing Act of 1937) school district, special district, intrastate...

  13. 28 CFR 551.71 - Procedures.

    Science.gov (United States)

    2010-07-01

    ... Administering of Polygraph Test § 551.71 Procedures. (a) The Warden may permit polygraph tests in connection with a State or Federal criminal felony investigation. (b) The Warden may permit polygraph tests in... polygraph test at the request of a defense counsel or other representative of the inmate. These requests...

  14. 9 CFR 145.71 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR BREEDING POULTRY Special Provisions for Primary Egg-Type Chicken Breeding Flocks and Products § 145.71 Definitions. Except where the context otherwise...: Chicks. Newly hatched chickens. Primary egg-type chicken breeding flocks. Foundation flocks that...

  15. 48 CFR 1503.500-71 - Procedures.

    Science.gov (United States)

    2010-10-01

    ... IMPROPER BUSINESS PRACTICES AND PERSONAL CONFLICTS OF INTEREST Contractor Responsibility To Avoid Improper Business Practices 1503.500-71 Procedures. (a) A contractor's system of management controls should provide for: (1) A written code of business ethics and conduct and an ethics training program for...

  16. 37 CFR 41.71 - Rebuttal brief.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Rebuttal brief. 41.71 Section... Rebuttal brief. (a) Within one month of the examiner's answer, any appellant may once file a rebuttal brief. (b)(1) The rebuttal brief of the owner may be directed to the examiner's answer and/or any...

  17. 50 CFR 71.12 - General provisions.

    Science.gov (United States)

    2010-10-01

    ...) MANAGEMENT OF FISHERIES CONSERVATION AREAS HUNTING AND FISHING ON NATIONAL FISH HATCHERY AREAS Fishing § 71.12 General provisions. The following provisions shall apply to public sport fishing on a national... may be amended as needed to meet management responsibilities for the area....

  18. 14 CFR 33.71 - Lubrication system.

    Science.gov (United States)

    2010-01-01

    ... Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Design and Construction; Turbine Aircraft Engines § 33.71 Lubrication system. (a... in which an aircraft is expected to operate. (b) Oil strainer or filter. There must be an...

  19. 40 CFR 71.2 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... fermentation included in NAICS codes 325193 or 312140; (xxi) Fossil-fuel boilers (or combination thereof... plants; (xxiv) Glass fiber processing plants; (xxv) Charcoal production plants; (xxvi) Fossil-fuel-fired... 40 Protection of Environment 15 2010-07-01 2010-07-01 false Definitions. 71.2 Section...

  20. 42 CFR 424.71 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM CONDITIONS FOR MEDICARE PAYMENT Limitations on Assignment and Reassignment of Claims § 424.71... hospital or other institution that furnishes health care services to inpatients. Entity means a...

  1. 49 CFR 572.71 - General description.

    Science.gov (United States)

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES 6-Year-Old Child § 572.71 General description. (a) The representative 6-year-old dummy consists of a drawings and specifications..., “Six-Year-Old Size Child Test Dummy SA106C,” October 28, 1991. (b) The dummy is made up of...

  2. 15 CFR 922.71 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Channel Islands National Marine Sanctuary § 922.71 Definitions. In addition to those definitions found at 15 CFR 922.3, the following definitions apply to this subpart... into which altered genetic matter, or genetic matter from another species, has been transferred...

  3. 21 CFR 71.1 - Petitions.

    Science.gov (United States)

    2010-04-01

    ... use of the color additive in packaging material, the petitioner shall show how this may occur and what... AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVE PETITIONS General Provisions § 71.1 Petitions. (a) Any interested person may propose the listing of a color...

  4. 9 CFR 71.1 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... Note: For Federal Register citations affecting § 71.1, see the List of CFR Sections Affected, which... methods approved by the Administrator, including, but not limited to, official tags, tattoos, and... authority. Official swine tattoo. A tattoo, conforming to the six-character alpha-numeric National...

  5. 45 CFR 400.71 - Definitions.

    Science.gov (United States)

    2010-10-01

    ... the employability or work skills of the individual. Employable means not exempt from registration for... Employability Services and Employment § 400.71 Definitions. For purposes of this subpart and Subpart I... must be determined acceptable by the State. Employability plan means an individualized written plan...

  6. 21 CFR 201.71 - Magnesium labeling.

    Science.gov (United States)

    2010-04-01

    ...-restricted diet”. The warnings in §§ 201.64(c), 201.70(c), 201.71(c), and 201.72(c) may be combined, if...-restricted diet. 1 See § 201.66(b)(4) of this chapter for definition of bullet symbol. (d) Any...

  7. Description of the B-71 library system

    International Nuclear Information System (INIS)

    The B-71 system of libraries for programming in machine codes for M-220 and BESM-4 computers is described. 22 libraries are described containing more than 50 programs both newly created libraries and libraries included in the B-61 system

  8. Aerial gamma ray and magnetic survey: Mississippi and Florida airborne survey, Russellville quadrangle, Arkansas

    International Nuclear Information System (INIS)

    The Russellville quadrangle in north central Arkansas overlies thick Paleozoic sediments of the Arkoma Basin. These Paleozoics dominate surface exposure except where covered by Quaternary alluvial materials. Examination of available literature shows no known uranium deposits (or occurrences) within the quadrangle. Eighty-eight groups of uranium samples were defined as anomalies and are discussed briefly. None were considered significant, and most appeared to be of cultural origin. Magnetic data show character that suggest structural and/or lithologic complexity, but imply relatively deep-seated sources

  9. 27 CFR 71.4 - Delegations of the Administrator.

    Science.gov (United States)

    2010-04-01

    ... officers are specified in TTB Order 1135.71, Delegation of the Administrator's Authorities in 27 CFR Part... Administrator. 71.4 Section 71.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU... and Construction of Regulations § 71.4 Delegations of the Administrator. Most of the...

  10. Construction and characterization of infectious cDNA clones of enterovirus 71(EV71)

    Institute of Scientific and Technical Information of China (English)

    Ying-wei; Ma; Shu-bin; Hao; Le-le; Sun; Jing; Li; Qiao; Qiao; Feng; Gao; Li; Zhao; Xue-jie; Yu; Zhi-yu; Wang; Hong-ling; Wen

    2015-01-01

    Dear Editor,Enterovirus 71(EV71),a member of the genus Enterovirus of the family Picornaviridae,is a single-stranded,positive-sense RNA virus that usually causes mild handfoot-mouth disease(HFMD)in children,with symptoms such as fever,diarrhea,and herpangina(Liu et al.,2013).However,certain strains of EV71 infection can cause severe neurological complications,such as SDLY107(Sun et al.,2014).EV71 is classified into three distinct genotypes(A–C);the B and C genotypes are further divided into B1–B5 and C1–C5 genotypes,respectively,based on

  11. Occurrence and distribution of dissolved solids, selenium, and uranium in groundwater and surface water in the Arkansas River Basin from the headwaters to Coolidge, Kansas, 1970-2009

    Science.gov (United States)

    Miller, Lisa D.; Watts, Kenneth R.; Ortiz, Roderick F.; ,

    2010-01-01

    In 2007, the U.S. Geological Survey (USGS), in cooperation with City of Aurora, Colorado Springs Utilities, Colorado Water Conservation Board, Lower Arkansas Valley Water Conservancy District, Pueblo Board of Water Works, Southeastern Colorado Water Activity Enterprise, Southeastern Colorado Water Conservancy District, and Upper Arkansas Water Conservancy District began a retrospective evaluation to characterize the occurrence and distribution of dissolved-solids (DS), selenium, and uranium concentrations in groundwater and surface water in the Arkansas River Basin based on available water-quality data collected by several agencies. This report summarizes and characterizes available DS, dissolved-selenium, and dissolved-uranium concentrations in groundwater and surface water for 1970-2009 and describes DS, dissolved-selenium, and dissolved-uranium loads in surface water along the main-stem Arkansas River and selected tributary and diversion sites from the headwaters near Leadville, Colorado, to the USGS 07137500 Arkansas River near Coolidge, Kansas (Ark Coolidge), streamgage, a drainage area of 25,410 square miles. Dissolved-solids concentrations varied spatially in groundwater and surface water in the Arkansas River Basin. Dissolved-solids concentrations in groundwater from Quaternary alluvial, glacial drift, and wind-laid deposits (HSU 1) increased downgradient with median values of about 220 mg/L in the Upper Arkansas subbasin (Arkansas River Basin from the headwaters to Pueblo Reservoir) to about 3,400 mg/L in the Lower Arkansas subbasin (Arkansas River Basin from John Martin Reservoir to Ark Coolidge). Dissolved-solids concentrations in the Arkansas River also increased substantially in the downstream direction between the USGS 07086000 Arkansas River at Granite, Colorado (Ark Granite), and Ark Coolidge streamgages. Based on periodic data collected from 1976-2007, median DS concentrations in the Arkansas River ranged from about 64 mg/L at Ark Granite to about

  12. Progress on the research and development of human enterovirus 71 (EV71) vaccines.

    Science.gov (United States)

    Liang, Zhenglun; Mao, Qunying; Gao, Fan; Wang, Junzhi

    2013-03-01

    Enterovirus 71 (EV71) infections, which can cause severe complications, have become one of the serious public health issues in the Western Pacific region and China. To date, a number of pharmaceutical companies and institutes have initiated the research and development of EV71 vaccines as a countermeasure. As is the case with innovative vaccine development, there are several critical bottlenecks in EV71 vaccine development that must be overcome before the clinical trials, including the selection of vaccine strain, standardization of the procedure for quantifying neutralizing antibody (NTAb) and antigen, establishment and application of a reference standard and biological standards, development of animal models for the evaluation of protective efficacy, and identification of the target patient population. To tackle these technical obstacles, researchers in Mainland of China have conducted a series of studies concerning the screening of vaccine strains and the establishment of criteria, biological standards and detection methods, thereby advancing EV71 vaccine development. This review summarizes recent worldwide progress on the quality control and evaluation of EV71 vaccines.

  13. Water quality of least-impaired lakes in eastern and southern Arkansas.

    Science.gov (United States)

    Justus, Billy

    2010-09-01

    A three-phased study identified one least-impaired (reference) lake for each of four Arkansas lake classifications: three classifications in the Mississippi Alluvial Plain (MAP) ecoregion and a fourth classification in the South Central Plains (SCP) ecoregion. Water quality at three of the least-impaired lakes generally was comparable and also was comparable to water quality from Kansas and Missouri reference lakes and Texas least-impaired lakes. Water quality of one least-impaired lake in the MAP ecoregion was not as good as water quality in other least-impaired lakes in Arkansas or in the three other states: a probable consequence of all lakes in that classification having a designated use as a source of irrigation water. Chemical and physical conditions for all four lake classifications were at times naturally harsh as limnological characteristics changed temporally. As a consequence of allochthonous organic material, oxbow lakes isolated within watersheds comprised of swamps were susceptible to low dissolved oxygen concentrations to the extent that conditions would be limiting to some aquatic biota. Also, pH in lakes in the SCP ecoregion was lakes exceeded that of shallow lakes. N/P ratios and trophic state indices may be less effective for assessing water quality for shallow lakes (lakes because there is an increased exposure of sediment (and associated phosphorus) to disturbance and light in the former. PMID:19705289

  14. Curriculum alignment and higher order thinking in introductory biology in Arkansas public two-year colleges

    Science.gov (United States)

    Crandall, Elizabeth Diane

    This dissertation identified the cognitive levels of lecture objectives, lab objectives, and test questions in introductory majors' biology. The study group included courses offered by 27 faculty members at 18 of the 22 community colleges in Arkansas. Using Bloom's Taxonomy to identify cognitive levels, the median lecture learning outcomes were at level 2 (Comprehension) and test assessments at Level 1 (Knowledge). Lab learning outcomes were determined to have a median of level 3 (Analysis). A correlation analysis was performed using SPSS software to determine if there was an association between the Bloom's level of lecture objectives and test assessments. The only significant difference found was at the Analysis level, or Bloom's level 4 (p=.043). Correlation analyses were run for two other data sets. Years of college teaching experience and hours of training in writing objectives and assessments were compared to the Bloom's Taxonomy level of lecture objectives and test items. No significant difference was found for either of these independent variables. This dissertation provides Arkansas two-year college biology faculty with baseline information about the levels of cognitive skills that are required in freshman biology for majors courses. It can serve to initiate conversations about where we are compared to a national study, where we need to be, and how we get there.

  15. EPSPS gene amplification in glyphosate-resistant in Italian ryegrass (Lolium perenne ssp. multiflorum) populations from Arkansas, USA

    Science.gov (United States)

    Glyphosate-resistant Italian ryegrass was detected in Arkansas, USA in 2007. In 2014, 45 populations were confirmed resistant in eight counties across the state. The level of resistance and resistance mechanisms in six populations was studied to assess the severity of the problem and identify altern...

  16. User's manual for FORAR: a stand model for composition and growth of upland forests of southern Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Mielke, D. L.; Shugart, H. H.; West, D. C.

    1977-04-01

    This report is a user's manual for FORAR, a computer model simulating stand growth and composition of upland forests of south central Arkansas. The model computes: the number and biomass of each tree species, and the dbh, age, and species of each individual tree on a 1/12-ha circular plot.

  17. The Effect of Performance-Pay in Little Rock, Arkansas on Student Achievement. Working Paper 2008-02

    Science.gov (United States)

    Winters, Marcus; Greene, Jay P.; Ritter, Gary; Marsh, Ryan

    2008-01-01

    This paper examines evidence from a performance-pay program implemented in five Little Rock, Arkansas elementary schools between 2004 and 2007. Using a differences-in-differences approach, the evidence shows that students whose teachers were eligible for performance pay made substantially larger test score gains in math, reading, and language than…

  18. 75 FR 36538 - Arkansas: Final Authorization of State-initiated Changes and Incorporation by Reference of State...

    Science.gov (United States)

    2010-06-28

    ... August 15, 2007, Federal Register authorization documents for Arkansas. The Solid Waste Disposal Act, as...''. 260.10 ``Solid waste management unit'' No Federal analog. or ``SWMU''''. 260.20(b) intro 260.20(b... and Ecology at 64 FR 63209-63213. (APC&E) Regulation Number 23 (Hazardous Waste Management)...

  19. Reciprocal Regulation between Enterovirus 71 and the NLRP3 Inflammasome

    Directory of Open Access Journals (Sweden)

    Hongbin Wang

    2015-07-01

    Full Text Available Enterovirus 71 (EV71 is the major etiological agent of hand, foot, and mouth disease (HFMD. Early studies showed that EV71-infected patients with severe complications exhibited elevated plasma levels of IL-1β, indicating that EV71 may activate inflammasomes. Our current study demonstrates that the NLRP3 inflammasome plays a protective role against EV71 infection of mice in vivo. EV71 replication in myeloid cells results in the activation of the NLRP3 inflammasome and secretion of IL-1β. Conversely, EV71 counteracts inflammasome activation through cleavage of NLRP3 by viral proteases 2A and 3C, which cleave NLRP3 protein at the G493-L494 or Q225-G226 junction, respectively. Moreover, EV71 3C interacts with NLRP3 and inhibits IL-1β secretion when expressed in mammalian cells. These results thus reveal a set of reciprocal regulations between enterovirus 71 and the NLRP3 inflammasome.

  20. EV71 vaccine, an invaluable gift for children

    OpenAIRE

    Liang, Zhenglun; Wang, Junzhi

    2014-01-01

    Enterovirus 71 (EV71) is a major pathogen for severe hand, foot and mouth disease (HFMD). Development of vaccines against EV71 would be the most effective approach to prevent the EV71 outbreak. Research and development (R&D) of EV71 vaccine was carried out in several Asian countries. Currently three companies in mainland China have completed Phase III clinical trials of inactivated EV71 whole-virus vaccines, whereas the other two companies have completed Phase I clinical trials separately in ...

  1. Crystal structures of enterovirus 71 (EV71) recombinant virus particles provide insights into vaccine design.

    Science.gov (United States)

    Lyu, Ke; Wang, Guang-Chuan; He, Ya-Ling; Han, Jian-Feng; Ye, Qing; Qin, Cheng-Feng; Chen, Rong

    2015-02-01

    Hand-foot-and-mouth disease (HFMD) remains a major health concern in the Asia-Pacific regions, and its major causative agents include human enterovirus 71 (EV71) and coxsackievirus A16. A desirable vaccine against HFMD would be multivalent and able to elicit protective responses against multiple HFMD causative agents. Previously, we have demonstrated that a thermostable recombinant EV71 vaccine candidate can be produced by the insertion of a foreign peptide into the BC loop of VP1 without affecting viral replication. Here we present crystal structures of two different naturally occurring empty particles, one from a clinical C4 strain EV71 and the other from its recombinant virus containing an insertion in the VP1 BC loop. Crystal structure analysis demonstrated that the inserted foreign peptide is well exposed on the particle surface without significant structural changes in the capsid. Importantly, such insertions do not seem to affect the virus uncoating process as illustrated by the conformational similarity between an uncoating intermediate of another recombinant virus and that of EV71. Especially, at least 18 residues from the N terminus of VP1 are transiently externalized. Altogether, our study provides insights into vaccine development against HFMD.

  2. Aliens or the SR-71 Blackbird?

    Science.gov (United States)

    Ruiz, Michael J.

    2006-04-01

    Unidentified flying objects (UFOs) capture the interest of our students and the general public.1,2 Here is a UFO story I tell my students to illustrate the workings of science. Science, at its most fundamental level, is observation and an attempt to analyze what is observed. But what happens when an observation cannot be explained with the available knowledge at the time? What do we do? The UFO sighting of my story could not be explained until disclosure of a top-secret military aircraft, the SR-71, also known as the "Blackbird."

  3. Protective effect of enterovirus‑71 (EV71) virus‑like particle vaccine against lethal EV71 infection in a neonatal mouse model.

    Science.gov (United States)

    Cao, Lei; Mao, Fengfeng; Pang, Zheng; Yi, Yao; Qiu, Feng; Tian, Ruiguang; Meng, Qingling; Jia, Zhiyuan; Bi, Shengli

    2015-08-01

    Enterovirus-71 (EV71) is a viral pathogen that causes severe cases of hand, foot and mouth disease (HFMD) among young children, with significant mortality. Effective vaccines against HFMD are urgently required. Several EV71 virus-like particle (VLP) vaccine candidates were found to be protective in the neonatal mouse EV71 challenge model. However, to what extent the VLP vaccine protects susceptible organs against EV71 infection in vivo has remained elusive. In the present study, the comprehensive immunogenicity of a potential EV71 vaccine candidate based on VLPs was evaluated in a neonatal mouse model. Despite lower levels of neutralizing antibodies to EV71 in the sera of VLP-immunized mice compared with those in mice vaccinated with inactivated EV71, the VLP-based vaccine was shown to be able to induce immunoglobulin (Ig)G and IgA memory-associated cellular immune responses to EV71. Of note, the EV71 VLP vaccine candidate was capable of inhibiting viral proliferation in cardiac muscle, skeletal muscle, lung and intestine of immunized mice and provided effective protection against the pathological damage caused by viral attack. In particular, the VLP vaccine was able to inhibit the transportation of EV71 from the central nervous system to the muscle tissue and greatly protected muscle tissue from infection, along with recovery from the viral infection. This led to nearly 100% immunoprotective efficacy, enabling neonatal mice delivered by VLP-immunized female adult mice to survive and grow with good health. The present study provided valuable additional knowledge of the specific protective efficacy of the EV71 VLP vaccine in vivo, which also indicated that it is a promising potential candidate for being developed into an EV71 vaccine.

  4. Geodatabase of the available top and bottom surface datasets that represent the Edwards-Trinity aquifer system, Arkansas, Oklahoma, and Texas

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This geodatabase contains the spatial datasets that represent the Edwards-Trinity aquifer system in the States of Arkansas, Oklahoma, and Texas. Included are: (1)...

  5. The Antiviral Effect of Baicalin on Enterovirus 71 In Vitro

    Directory of Open Access Journals (Sweden)

    Xiang Li

    2015-08-01

    Full Text Available Baicalin is a flavonoid compound extracted from Scutellaria roots that has been reported to possess antibacterial, anti-inflammatory, and antiviral activities. However, the antiviral effect of baicalin on enterovirus 71 (EV71 is still unknown. In this study, we found that baicalin showed inhibitory activity on EV71 infection and was independent of direct virucidal or prophylactic effect and inhibitory viral absorption. The expressions of EV71/3D mRNA and polymerase were significantly blocked by baicalin treatment at early stages of EV71 infection. In addition, baicalin could decrease the expressions of FasL and caspase-3, as well as inhibit the apoptosis of EV71-infected human embryonal rhabdomyosarcoma (RD cells. Altogether, these results indicate that baicalin exhibits potent antiviral effect on EV71 infection, probably through inhibiting EV71/3D polymerase expression and Fas/FasL signaling pathways.

  6. Transgenic mouse model for the study of enterovirus 71 neuropathogenesis

    OpenAIRE

    Fujii, Ken; Nagata, Noriyo; Sato, Yuko; Ong, Kien Chai; Wong, Kum Thong; Yamayoshi, Seiya; Shimanuki, Midori; Shitara, Hiroshi; Taya, Choji; KOIKE, Satoshi

    2013-01-01

    EV71 infection with severe neurological complications has become a serious public health concern. However, suitable small animal models to study human EV71 pathogenesis are not available. We have generated a Tg mouse model by expressing the human EV71 receptor, Scavenger receptor B2, and found it to be susceptible to EV71 infection. This Tg mouse model exhibits neurological disease and pathology very similar to that observed in humans. The results confirm that the Scavenger receptor B2 recept...

  7. A COLLABORATIVE LEARNING MATRIX FOR COMBINING SCIENCE WITH STAKEHOLDER INVOLVEMENT TO PRIORITIZE WATERSHED IMPLEMENTATION IN ARKANSAS' NONPOINT SOURCE STATE MANAGEMENT PLAN

    OpenAIRE

    ROBERT MORGAN; MARTY MATLOCK

    2008-01-01

    In 2004, the Ecological Engineering Group at the University of Arkansas received a grant to update Arkansas' nonpoint source pollution (NPS) management program. A stakeholder involvement process was developed that used collaborative learning (CL) and comparative risk assessment (CRA) to prioritise watersheds for NPS implementation. The relative ecological risk posed by nonpoint pollution to each watershed was assessed and values assigned using available water quality, GIS, and demographic dat...

  8. Demographics of Same-sex Couples in Arkansas, Missouri, Nebraska, North Dakota, and South Dakota: Analyses of the 2013 American Community Survey

    OpenAIRE

    Gates, Gary J.

    2015-01-01

    Analyzing data from the 2013 US American Community Survey, this report considers the demographic, economic, and geographic characteristics of same-sex couples (married and unmarried), especially those raising children, in Arkansas, Missouri, Nebraska, North Dakota, and South Dakota. Comparisons are made with their different-sex counterparts. In Arkansas, Missouri, Nebraska, North Dakota, and South Dakota, as of 2013, there are an estimated 19652 same-sex couples. An estimated 12% of these cou...

  9. 7 CFR 1794.71 - Adoption of an EA.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 12 2010-01-01 2010-01-01 false Adoption of an EA. 1794.71 Section 1794.71... AGRICULTURE (CONTINUED) ENVIRONMENTAL POLICIES AND PROCEDURES Adoption of Environmental Documents § 1794.71 Adoption of an EA. RUS may adopt a Federal EA or EIS or a portion thereof as its EA. RUS shall make the...

  10. 48 CFR 1852.237-71 - Pension portability.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Pension portability. 1852.237-71 Section 1852.237-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... 1852.237-71 Pension portability. As prescribed at 1837.110-70(b), insert the following clause:...

  11. 41 CFR 105-71.125 - Program income.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Program income. 105-71... GOVERNMENTS 71.12-Post-Award Requirements/Financial Administration § 105-71.125 Program income. (a) General. Grantees are encouraged to earn income to defray program costs. Program income includes income from...

  12. 10 CFR 71.107 - Package design control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Package design control. 71.107 Section 71.107 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Quality Assurance § 71.107 Package design control. (a) The licensee, certificate holder, and applicant for a...

  13. 14 CFR 71.7 - Bearings, radials, and mileages.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Bearings, radials, and mileages. 71.7 Section 71.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... REPORTING POINTS § 71.7 Bearings, radials, and mileages. All bearings and radials in this part are true...

  14. 21 CFR 101.71 - Health claims: claims not authorized.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Health claims: claims not authorized. 101.71 Section 101.71 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION FOOD LABELING Specific Requirements for Health Claims § 101.71...

  15. 29 CFR 71.13 - Employee standards of conduct.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Employee standards of conduct. 71.13 Section 71.13 Labor... ACT OF 1974 General § 71.13 Employee standards of conduct. (a) Each component shall inform its... maintains no system of records without public notice. (b) Except to the extent that the Privacy Act...

  16. 30 CFR 71.601 - Drinking water; quality.

    Science.gov (United States)

    2010-07-01

    ... drinking water provided shall conform to the Public Health Service Drinking Water Standards, 42 CFR part 72... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Drinking water; quality. 71.601 Section 71.601... Water § 71.601 Drinking water; quality. (a) Potable water provided in accordance with the provisions...

  17. 46 CFR 71.75-15 - Posting of Convention certificates.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Posting of Convention certificates. 71.75-15 Section 71... AND CERTIFICATION Certificates Under the International Convention for Safety of Life at Sea, 1960 § 71.75-15 Posting of Convention certificates. (a) The certificates described in this subpart,...

  18. 5 CFR 7.1 - Discretion in filling vacancies.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Discretion in filling vacancies. 7.1 Section 7.1 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE RULES GENERAL PROVISIONS (RULE VII) § 7.1 Discretion in filling vacancies. In his discretion, an appointing officer may fill...

  19. 42 CFR 71.11 - Bills of health.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Bills of health. 71.11 Section 71.11 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES QUARANTINE, INSPECTION, LICENSING FOREIGN QUARANTINE Measures at Foreign Ports § 71.11 Bills of health. A carrier at any foreign port clearing...

  20. 10 CFR 71.127 - Handling, storage, and shipping control.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Handling, storage, and shipping control. 71.127 Section 71... MATERIAL Quality Assurance § 71.127 Handling, storage, and shipping control. The licensee, certificate... handling, storage, shipping, cleaning, and preservation of materials and equipment to be used in...

  1. 19 CFR 123.71 - Description of program.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Description of program. 123.71 Section 123.71 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY CUSTOMS RELATIONS WITH CANADA AND MEXICO Land Border Carrier Initiative Program § 123.71 Description of program. The Land Border...

  2. 12 CFR 908.71 - Practice before the Finance Board.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Practice before the Finance Board. 908.71 Section 908.71 Banks and Banking FEDERAL HOUSING FINANCE BOARD FEDERAL HOUSING FINANCE BOARD ORGANIZATION... Finance Board § 908.71 Practice before the Finance Board. Practice before the Finance Board for...

  3. 28 CFR 71.5 - Review by the reviewing official.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Review by the reviewing official. 71.5... Justice § 71.5 Review by the reviewing official. (a) If, based on the report of the investigating official under § 71.4(b), the reviewing official determines that there is adequate evidence to believe that...

  4. 36 CFR 71.11 - Collection of Federal recreation fees.

    Science.gov (United States)

    2010-07-01

    ... recreation fees. 71.11 Section 71.11 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR RECREATION FEES § 71.11 Collection of Federal recreation fees. The bureaus of the Department of the Interior administering outdoor recreation programs shall provide for the collection...

  5. 20 CFR 71.6 - Notice of injury or death.

    Science.gov (United States)

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Notice of injury or death. 71.6 Section 71.6..., DISABILITY OR DEATH OF CIVILIAN AMERICAN CITIZENS INCURRED WHILE DETAINED BY OR IN HIDING FROM THE IMPERIAL JAPANESE GOVERNMENT GENERAL PROVISIONS § 71.6 Notice of injury or death. Notwithstanding any of...

  6. 46 CFR 71.25-37 - Pollution prevention.

    Science.gov (United States)

    2010-10-01

    ... pollution prevention in 33 CFR part 155, subpart B. ... 46 Shipping 3 2010-10-01 2010-10-01 false Pollution prevention. 71.25-37 Section 71.25-37 Shipping... Annual Inspection § 71.25-37 Pollution prevention. At each inspection for certification, the...

  7. 42 CFR 71.51 - Dogs and cats.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Dogs and cats. 71.51 Section 71.51 Public Health... QUARANTINE Importations § 71.51 Dogs and cats. (a) Definitions. As used in this section the term: Cat means all domestic cats. Confinement means restriction of a dog or cat to a building or other enclosure at...

  8. 29 CFR 4044.71 - Valuation of annuity benefits.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Valuation of annuity benefits. 4044.71 Section 4044.71... ALLOCATION OF ASSETS IN SINGLE-EMPLOYER PLANS Valuation of Benefits and Assets Non-Trusteed Plans § 4044.71 Valuation of annuity benefits. The value of a benefit which is to be paid as an annuity is the cost...

  9. 10 CFR 71.31 - Contents of application.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Contents of application. 71.31 Section 71.31 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Application for Package Approval § 71.31 Contents of application. (a) An application for an approval under...

  10. Phenotypic and genotypic characteristics of novel mouse cell line (NIH/3T3-adapted human enterovirus 71 strains (EV71:TLLm and EV71:TLLmv.

    Directory of Open Access Journals (Sweden)

    Carla Bianca Luena Victorio

    Full Text Available Since its identification in 1969, Enterovirus 71 (EV71 has been causing periodic outbreaks of infection in children worldwide and most prominently in the Asia-Pacific Region. Understanding the pathogenesis of Enterovirus 71 (EV71 is hampered by the virus's inability to infect small animals and replicate in their derived in vitro cultured cells. This manuscript describes the phenotypic and genotypic characteristics of two selected EV71 strains (EV71:TLLm and EV71:TLLmv, which have been adapted to replicate in mouse-derived NIH/3T3 cells, in contrast to the original parental virus which is only able to replicate in primate cell lines. The EV71:TLLm strain exhibited productive infection in all primate and rodent cell lines tested, while EV71:TLLmv exhibited greater preference for mouse cell lines. EV71:TLLmv displayed higher degree of adaptation and temperature adaptability in NIH/3T3 cells than in Vero cells, suggesting much higher fitness in NIH/3T3 cells. In comparison with the parental EV71:BS strain, the adapted strains accumulated multiple adaptive mutations in the genome resulting in amino acid substitutions, most notably in the capsid-encoding region (P1 and viral RNA-dependent RNA polymerase (3D. Two mutations, E167D and L169F, were mapped to the VP1 canyon that binds the SCARB2 receptor on host cells. Another two mutations, S135T and K140I, were located in the VP2 neutralization epitope spanning amino acids 136-150. This is the first report of human EV71 with the ability to productively infect rodent cell lines in vitro.

  11. Phenotypic and genotypic characteristics of novel mouse cell line (NIH/3T3)-adapted human enterovirus 71 strains (EV71:TLLm and EV71:TLLmv).

    Science.gov (United States)

    Victorio, Carla Bianca Luena; Xu, Yishi; Ng, Qimei; Chow, Vincent T K; Chua, Kaw Bing

    2014-01-01

    Since its identification in 1969, Enterovirus 71 (EV71) has been causing periodic outbreaks of infection in children worldwide and most prominently in the Asia-Pacific Region. Understanding the pathogenesis of Enterovirus 71 (EV71) is hampered by the virus's inability to infect small animals and replicate in their derived in vitro cultured cells. This manuscript describes the phenotypic and genotypic characteristics of two selected EV71 strains (EV71:TLLm and EV71:TLLmv), which have been adapted to replicate in mouse-derived NIH/3T3 cells, in contrast to the original parental virus which is only able to replicate in primate cell lines. The EV71:TLLm strain exhibited productive infection in all primate and rodent cell lines tested, while EV71:TLLmv exhibited greater preference for mouse cell lines. EV71:TLLmv displayed higher degree of adaptation and temperature adaptability in NIH/3T3 cells than in Vero cells, suggesting much higher fitness in NIH/3T3 cells. In comparison with the parental EV71:BS strain, the adapted strains accumulated multiple adaptive mutations in the genome resulting in amino acid substitutions, most notably in the capsid-encoding region (P1) and viral RNA-dependent RNA polymerase (3D). Two mutations, E167D and L169F, were mapped to the VP1 canyon that binds the SCARB2 receptor on host cells. Another two mutations, S135T and K140I, were located in the VP2 neutralization epitope spanning amino acids 136-150. This is the first report of human EV71 with the ability to productively infect rodent cell lines in vitro.

  12. Size distribution of planktonic autotrophy and microheterotrophy in DeGray Reservoir, Arkansas

    International Nuclear Information System (INIS)

    Naturally occurring assemblages of phytoplankton and bacterioplankton were radiolabelled with sodium 14C-bicarbonate and sodium 3H-acetate and size fractionated to determine the size structure of planktonic autotrophy and microheterotrophy in DeGray Reservoir, an oligotrophic impoundment of the Caddo River in south-central Arkansas. Size distributions of autotrophy and microheterotrophy were remarkably uniform seasonally, vertically within the water column, and along the longitudinal axis of the reservoir despite significant changes in environmental conditions. Planktonic autotrophy was dominated by small algal cells with usually >50% of the photosynthetic carbon uptake accounted for by organisms 75% of the planktonic microheterotrophy. Longitudinal patterns in autotrophic and microheterotrophic activities associated with >3-μm and >1-μm size fractions, respectively, suggest an uplake to downlake shift from riverine to lacustrine environmental influences within the reservoir. 83 references, 7 figures

  13. Ecological Factors Affecting the Distribution of Woody Vegetation Near the Arkansas River, Tulsa County

    Directory of Open Access Journals (Sweden)

    Anne Wanamnaker Long

    2004-12-01

    Full Text Available Ecological factors affecting plant distribution were studied over different rock strata and slope exposures above the Arkansas River, Tulsa County. Here the Wann sandstone caprock is underlain by the Iola limestone formation. The vegetation was analyzed taxonomically by a complete collection throughout one growing season. Belt transects crossing rock strata on all slope exposures permitted computation of parameters summarized by an Importance Percentage for each woody species. Differences in species populations and degree of mesophytism exist on the slope exposures. Sandstone upland dominants are post and blackjack oaks. Smoke-tree, rare in Oklahoma, and chinquapin oak are closely associated in limestone microhabitats, where each occupies a separate niche. The smoke-tree, of disjunct distribution, appears to be a relict of widespread occurrence in past geologic periods. Its survival with limited ecological amplitude is due to the continuance of the microhabitats to which it is so well adapted.

  14. Digital surfaces and thicknesses of selected hydrogeologic units within the Ozark Plateaus aquifer system, northwestern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Bolyard, Susan E.; Hart, Rheannon M.; Clark, Jimmy M.

    2014-01-01

    Digital surfaces and thicknesses of nine hydrogeologic units of the Ozark Plateaus aquifer system from land surface to the top of the Gunter Sandstone in northwestern Arkansas were created using geophysical logs, drillers’ logs, geologist-interpreted formation tops, and previously published maps. The 6,040 square mile study area in the Ozark Plateaus Province includes Benton, Washington, Carroll, Madison, Boone, Newton, Marion, and Searcy Counties. The top of each hydrogeologic unit delineated on geophysical logs was based partly on previously published reports and maps and also from drillers’ logs. These logs were then used as a basis to contour digital surfaces showing the top and thickness of the Fayetteville Shale, the Boone Formation, the Chattanooga Shale, the Everton Formation, the Powell Dolomite, the Cotter Dolomite, the Roubidoux Formation, the Gasconade Dolomite, and the Gunter Sandstone.

  15. Geochemical and geostatistical evaluation, Arkansas Canyon Planning Unit, Fremont and Custer Counties, Colorado

    Science.gov (United States)

    Weiland, E.F.; Connors, R.A.; Robinson, M.L.; Lindemann, J.W.; Meyer, W.T.

    1982-01-01

    A mineral assessment of the Arkansas Canyon Planning Unit was undertaken by Barringer Resources Inc., under the terms of contract YA-553-CTO-100 with the Bureau of Land Management, Colorado State Office. The study was based on a geochemical-geostatistical survey in which 700 stream sediment samples were collected and analyzed for 25 elements. Geochemical results were interpreted by statistical processing which included factor, discriminant, multiple regression and characteristic analysis. The major deposit types evaluated were massive sulfide-base metal, sedimentary and magmatic uranium, thorium vein, magmatic segregation, and carbonatite related deposits. Results of the single element data and multivariate geostatistical analysis indicate that limited potential exists for base metal mineralization near the Horseshoe, El Plomo, and Green Mountain Mines. Thirty areas are considered to be anomalous with regard to one or more of the geochemical parameters evaluated during this study. The evaluation of carbonatite related mineralization was restricted due to the lack of geochemical data specific to this environment.

  16. Geologic map of the west-central Buffalo National River region, northern Arkansas

    Science.gov (United States)

    Hudson, Mark R.; Turner, Kenzie J.

    2014-01-01

    This map summarizes the geology of the west-central Buffalo National River region in the Ozark Plateaus region of northern Arkansas. Geologically, the region lies on the southern flank of the Ozark dome, an uplift that exposes oldest rocks at its center in Missouri. Physiographically, the map area spans the Springfield Plateau, a topographic surface generally held up by Mississippian cherty limestone and the higher Boston Mountains to the south, held up by Pennsylvanian rocks. The Buffalo River flows eastward through the map area, enhancing bedrock erosion of an approximately 1,600-ft- (490-m-) thick sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. Quaternary surficial units are present as alluvial deposits along major streams, including a series of terrace deposits from the Buffalo River, as well as colluvium and landslide deposits mantling bedrock on hillslopes.

  17. Effects of recent climate variability on groundwater levels in eastern Arkansas

    Science.gov (United States)

    Czarnecki, John B.; Schrader, T.P.

    2013-01-01

    Water-level fluctuations in wells completed in the Mississippi River Valley alluvial aquifer in eastern Arkansas were compared to variability in annual precipitation, an indicator of climate variability. The wettest year on record in Little Rock, Arkansas, occurred in 2009 with 81.79 inches of precipitation compared to an average of 47.1 inches per year. In contrast, 2005 and 2010 were the 7th and 14th driest years on record with 34.55 and 36.52 inches per year, respectively. This variability in precipitation was reflected in water-level altitude changes between 2004 and 2008 and 2006 and 2010. Generally, drier conditions between 2004 and 2008 led to an average decline in water levels of 1.62 feet, whereas wetter conditions between 2006 and 2010 led to an average rise in water levels of 1.36 feet. Drier periods likely resulted in less recharge compared to wetter periods. Groundwater use from the alluvial aquifer peaked in 2000 and has since declined, in part, because of conservation measures and substantial reduction in aquifer saturated thickness. Groundwater-flow model results showed some areas of the alluvial aquifer simulated as dry in 2010, indicating a reduced capacity of the alluvial aquifer to produce water in those areas. Additional factors affecting groundwater use include the types of crops grown in an area and the availabitiliy of crop subsidies. Real-time continuous water-level measurements in wells allow for a more accurate assessment of the effect of variability in precipitation and water use than periodic water-level measurements.

  18. The road to tuberculosis (Mycobacterium tuberculosis elimination in Arkansas; a re-examination of risk groups.

    Directory of Open Access Journals (Sweden)

    Anna Berzkalns

    Full Text Available OBJECTIVES: This study was conducted to generate knowledge useful for developing public health interventions for more effective tuberculosis control in Arkansas. METHODS: The study population included 429 culture-confirmed reported cases (January 1, 2004-December 31, 2010. Mycobacterium tuberculosis genotyping data were used to identify cases likely due to recent transmission (clustered versus reactivation (non-clustered. Poisson regression models estimated average decline rate in incidence over time and assessed the significance of differences between subpopulations. A multinomial logistic model examined differences between clustered and non-clustered incidence. RESULTS: A significant average annual percent decline was found for the overall incidence of culture-confirmed (9%; 95% CI: 5.5%, 16.9%, clustered (6%; 95% CI: 0.5%, 11.6%, and non-clustered tuberculosis cases (12%; 95% CI: 7.6%, 15.9%. However, declines varied among demographic groups. Significant declines in clustered incidence were only observed in males, non-Hispanic blacks, 65 years and older, and the rural population. CONCLUSIONS: These findings suggest that the Arkansas tuberculosis control program must target both traditional and non-traditional risk groups for successful tuberculosis elimination. The present study also demonstrates that a thorough analysis of TB trends in different population subgroups of a given geographic region or state can lead to the identification of non-traditional risk factors for TB transmission. Similar studies in other low incidence populations would provide beneficial data for how to control and eventually eliminate TB in the U.S.

  19. Progress in the research of enterovirus 71%肠道病毒71型的研究进展

    Institute of Scientific and Technical Information of China (English)

    顾文珍

    2010-01-01

    Enterovirus 71 (EV71) is one of the main pathogens of hand, foot and mouth disease resulting in severe neurological complications and fatality. In fact, EV71 has been regarded as the most important neurotropic enterovirus after eradication of poliovirus. This paper reviews predisposing factors of EV71 infection and progress in the research of EV71 vaccine, thus pointing out the way for prevention and control of EV71 infection.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病(hand,foot and mouth disease,HFMD)的主要病原体之一,HFMD可并发严重神经系统疾病,甚至导致死亡.事实上,EV71一直被视为继脊髓灰质炎病毒消灭后最重要的嗜神经病毒.此文就EV71感染的易感因素以及疫苗的研究进展进行综述,从而为EV71感染的防控指明方向.

  20. Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication.

    Science.gov (United States)

    Kuo, Rei-Lin; Lin, Ya-Han; Wang, Robert Yung-Liang; Hsu, Chia-Wei; Chiu, Yi-Ting; Huang, Hsing-I; Kao, Li-Ting; Yu, Jau-Song; Shih, Shin-Ru; Wu, Chih-Ching

    2015-04-01

    Enterovirus 71 (EV71) is a human enterovirus that has seriously affected the Asia-Pacific area for the past two decades. EV71 infection can result in mild hand-foot-and-mouth disease and herpangina and may occasionally lead to severe neurological complications in children. However, the specific biological processes that become altered during EV71 infection remain unclear. To further explore host responses upon EV71 infection, we identified proteins differentially expressed in EV71-infected human glioblastoma SF268 cells using isobaric mass tag (iTRAQ) labeling coupled with multidimensional liquid chromatography-mass spectrometry (LC-MS/MS). Network analysis of proteins altered in cells infected with EV71 revealed that the changed biological processes are related to protein and ion transport, regulation of protein degradation, and homeostatic processes. We confirmed that the levels of NEDD4L and PSMF1 were increased and reduced, respectively, in EV71-infected cells compared to mock-infected control cells. To determine the physiological relevance of our findings, we investigated the consequences of EV71 infection in cells with NEDD4L or PSMF1 depletion. We found that the depletion of NEDD4L significantly reduced the replication of EV71, whereas PSMF1 knockdown enhanced EV71 replication. Collectively, our findings provide the first evidence of proteome-wide dysregulation by EV71 infection and suggest a novel role for the host protein NEDD4L in the replication of this virus.

  1. Characteristics and treatment strategies of severe enterovirus 71 (EV71) infection in children%小儿危重肠病毒71型(EV71)感染临床特征及救治策略

    Institute of Scientific and Technical Information of China (English)

    张育才; 徐梁; 戎群芳

    2010-01-01

    @@ 肠病毒71型(Enterovirus 71,EV71)是手足口病病原之一,也是发生危重病例最重要的病原.根据中国卫生部及疾病预防和控制中心资料,绝大多数死亡病例是由E71感染所致.重症EV71感染主要表现为脑干脑炎、脑脊髓炎、脊髓灰质炎样综合征等.

  2. The Construction of the EGFP Enterovirus 71%肠道病毒71型(EV71)荧光病毒的构建

    Institute of Scientific and Technical Information of China (English)

    朱守海; 王璐璐

    2014-01-01

    人肠道病毒71型(Enterovirus,EV71)是引起手足口病的主要病原体。首先利用反向遗传学的方法,构建了EV71全长cDNA感染性克隆,经体外转录、转染RD细胞后成功获得了拯救病毒。随后,将增强型绿色荧光蛋白基因(Enhanced green fluorescent protein,EGFP)插入到EV71基因组中构建荧光病毒。结果表明,此荧光病毒不仅可以侵染、复制,在传代的过程中EGFP基因也保持了较好的稳定性,表明其可以作为一种报告病毒应用于高通量的EV71抗病毒药物筛选中。%Human Enterovirus 71(EV71)is one of the major agents causing hand, foot and mouth disease(HFMD). An infectious full-length cDNA clone of EV71 as well as the recuse virus were obtained using the reverse genetic techniques. Then, the EGFP gene was inserted into the EV71 genome to construct the reporter virus. The results show that the EGFP-EV71 virus not only are replication-competent and infectious, but the EGFP gene are stable during passaging, implicating the recombinant virus could be used as a reporter virus for high throughput EV71 antiviral drugs screening.

  3. BEACON/MOD2A analysis of the Arkansas-1 reactor cavity during a hypothetical hot leg break

    International Nuclear Information System (INIS)

    As part of the evaluation of the new MOD2A version of the BEACON code, the Arkansas-1 reactor cavity was modeled during a hypothetical loss-of-coolant accident. Results of the BEACON analysis were compared with results obtained previously with the COMPARE containment code. Studies were also made investigating some of the BEACON interphasic, timestep control, and wall heat transfer options to assure that these models were working properly and to observe their effects on the results. Descriptions of the Arkansas-1 reactor cavity, initial assumptions during the hypothetical LOCA, and methods of modeling with BEACON are presented. Some of the problems encountered in accurately modeling the penetrations surrounding the hot and cold leg pipes are also discussed

  4. Statistical summary of selected water-quality data (water years 1975 through 1985) for Arkansas rivers and streams

    Science.gov (United States)

    Petersen, J.C.

    1988-01-01

    Descriptive statistics were calculated for selected water quality data for 116 water quality stations on Arkansas rivers and streams. Water quality properties summarized included pH, specific conductance, dissolved oxygen, total alkalinity, total hardness, common dissolved constituents, phosphorus, nitrogen, biochemical oxygen demand, bacteria, turbidity, suspended sediment, and several trace metals. Regression equations and related statistics describing the relation between specific conductance and total alkalinity and several dissolved constituents also were calculated. Typical water quality (based upon median values at individual stations) of physiographic sections and major rivers of Arkansas also is discussed. Discernible differences in water quality exist between sections and major rivers. The regression analysis indicated that the usefulness of specific conductance as a predictor of other water quality values is variable. The relation between specific conductance and the other property is not statistically significant (p>0.05) about 30% of the time. (USGS)

  5. 肠道病毒71型疫苗研究进展%Research progress of enterovirus 71 vaccines

    Institute of Scientific and Technical Information of China (English)

    袁晓晶; 温红玲; 王志玉

    2012-01-01

    Enterovirus 71 (EV71) is one of the main causative pathogens of hand,foot and mouth disease resulting in neurological complications and death.There are no effective drugs and measures for EV71 infection at present.Development of effective vaccines is the best method to control EV71 epidemic.This review summarizes the research progress of EV71 vaccines.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病(hand,foot and mouth disease,HFMD)的主要病原体之一,HFMD可并发神经系统疾病,甚至导致死亡.对于EV71感染,目前尚无有效的治疗药物和措施,因此研制有效的疫苗是控制EV71流行的最佳方法.此文就EV71疫苗的研究进展进行综述.

  6. Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry.

    Science.gov (United States)

    Xu, Fengwen; Zhao, Xiaoxiao; Hu, Siqi; Li, Jian; Yin, Lijuan; Mei, Shan; Liu, Tingting; Wang, Ying; Ren, Lili; Cen, Shan; Zhao, Zhendong; Wang, Jianwei; Jin, Qi; Liang, Chen; Ai, Bin; Guo, Fei

    2016-01-01

    Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection. PMID:27608771

  7. Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry

    Science.gov (United States)

    Xu, Fengwen; Zhao, Xiaoxiao; Hu, Siqi; Li, Jian; Yin, Lijuan; Mei, Shan; Liu, Tingting; Wang, Ying; Ren, Lili; Cen, Shan; Zhao, Zhendong; Wang, Jianwei; Jin, Qi; Liang, Chen; Ai, Bin; Guo, Fei

    2016-01-01

    Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection. PMID:27608771

  8. Radiotherapy for 71 patients with tonsillar carcinoma

    International Nuclear Information System (INIS)

    Objective: To evaluate the therapeutic outcome and determine the prognosticators of tonsillar carcinoma treated by different approaches. Methods: A retrospective study was done on 71 tonsillar carcinoma patients treated from August 1971 to October 1995 by three different ways of radiotherapy. All were pathologically proved. Fifteen patients received external bean therapy plus intracavitary brachytherapy (EBRT + BRT), 39 patients were treated by EBRT only (EBRT) and the other 17 patients underwent FBRT plus chemotherapy (EBTR + CHEMO). Results: The local control rates of EBRT + BRT, EBRT and EBRT + chemo were 82.1%, 68.4% and 64.2%. There was significant difference between EBRT + BRT and the other two groups (x2=4.65, P=0.030; x2=5.65, P=0.018). The 3-, 5- and 10- year survival rates of EBRT + BRT, EBRT and EBRT + CHEMO were 76.3%, 53.2%, 31.2%; 74.0%, 45.2%, 33.5% and 75.2%, 47.1%, 25.3%. All these survival rates showed no statistical significance. (P > 0.05). Conclusion: Brachytherapy, when used to increase the dose effect, may enhance the local effect without any change in the overall survival

  9. Subtask 7.1 - Strategic Studies

    Energy Technology Data Exchange (ETDEWEB)

    Thomas Erickson

    2009-03-30

    The Energy & Environmental Research Center (EERC) has recently completed 11 years of research through the Cooperative Agreement with the U.S. Department of Energy (DOE) National Energy Technology Laboratory (NETL) focused on fossil energy technology development and demonstration. To support a significant number of the different activities being considered within all of our research contracts with NETL, a subtask (7.1 Strategic Studies) was created to focus on small research efforts that came up throughout the year which would support an existing EERC-NETL project or would help to develop a new concept for inclusion in future efforts. Typical efforts conducted under this task were usually between $15,000 and $60,000 in scope and had time lines of less than 6 months. A limited number of larger studies were also conducted, generally at the direct request of NETL. Over the life of this task, 46 projects were conducted. These efforts ranged from quick experiments to gain fundamental knowledge to support a current effort, to literature reviews, to a few larger engineering efforts.

  10. Norepinephrine and Epinephrine Enhanced the Infectivity of Enterovirus 71.

    Directory of Open Access Journals (Sweden)

    Yu-Ting Liao

    Full Text Available Enterovirus 71 (EV71 infections may be associated with neurological complications, including brainstem encephalitis (BE. Severe EV71 BE may be complicated with autonomic nervous system (ANS dysregulation and/or pulmonary edema (PE. ANS dysregulation is related to the overactivation of the sympathetic nervous system, which results from catecholamine release.The aims of this study were to explore the effects of catecholamines on severe EV71 infection and to investigate the changes in the percentages of EV71-infected cells, virus titer, and cytokine production on the involvement of catecholamines.Plasma levels of norepinephrine (NE and epinephrine (EP in EV71-infected patients were measured using an enzyme-linked immunoassay. The expression of adrenergic receptors (ADRs on RD, A549, SK-N-SH, THP-1, Jurkat and human peripheral blood mononuclear cells (hPBMCs were detected using flow cytometry. The percentages of EV71-infected cells, virus titer, and cytokine production were investigated after treatment with NE and EP.The plasma levels of NE and EP were significantly higher in EV71-infected patients with ANS dysregulation and PE than in controls. Both α1A- and β2-ADRs were expressed on A549, RD, SK-N-SH, HL-60, THP-1, Jurkat cells and hPBMCs. NE treatment elevated the percentages of EV71-infected cells to 62.9% and 22.7% in THP-1 and Jurkat cells, respectively. Via treatment with EP, the percentages of EV71-infected cells were increased to 64.6% and 26.9% in THP-1 and Jurkat cells. The percentage of EV71-infected cells increased upon NE or EP treatment while the α- and β-blockers reduced the percentages of EV71-infected cells with NE or EP treatment. At least two-fold increase in virus titer was observed in EV71-infected A549, SK-N-SH and hPBMCs after treatment with NE or EP. IL-6 production was enhanced in EV71-infected hPBMCs at a concentration of 102 pg/mL NE.The plasma levels of NE and EP elevated in EV71-infected patients with ANS

  11. 新型肠道病毒71型(EV71)疫苗质量控制和评价标准的研究%Quality Control and Evaluation of New Enterovirus 71 (EV71) Vaccines

    Institute of Scientific and Technical Information of China (English)

    毛群颖; 姚昕; 梁争论

    2012-01-01

    目的 研究建立新型肠道病毒71型(EV71)疫苗质量控制标准和评价体系,为EV71疫苗研发提供基础.方法 根据创新型疫苗研发的要求,充分发挥技术优势,开展EV71疫苗质控标准、标准品和评价方法研究.结果与结论 建立EV71灭活疫苗的中和抗体和抗原定量测定标准品,统一不同企业疫苗的质控标准,规范疫苗的评价体系,为EV71疫苗的研发提供保障.

  12. Survey of Borreliae in ticks, canines, and white-tailed deer from Arkansas, U.S.A.

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    Fryxell Rebecca T

    2012-07-01

    Full Text Available Abstract Background In the Eastern and Upper Midwestern regions of North America, Ixodes scapularis (L. is the most abundant tick species encountered by humans and the primary vector of B. burgdorferi, whereas in the southeastern region Amblyomma americanum (Say is the most abundant tick species encountered by humans but cannot transmit B. burgdorferi. Surveys of Borreliae in ticks have been conducted in the southeastern United States and often these surveys identify B. lonestari as the primary Borrelia species, surveys have not included Arkansas ticks, canines, or white-tailed deer and B. lonestari is not considered pathogenic. The objective of this study was to identify Borrelia species within Arkansas by screening ticks (n = 2123, canines (n = 173, and white-tailed deer (n = 228 to determine the identity and locations of Borreliae endemic to Arkansas using PCR amplification of the flagellin (flaB gene. Methods Field collected ticks from canines and from hunter-killed white-tailed were identified to species and life stage. After which, ticks and their hosts were screened for the presence of Borrelia using PCR to amplify the flaB gene. A subset of the positive samples was confirmed with bidirectional sequencing. Results In total 53 (21.2% white-tailed deer, ten (6% canines, and 583 (27.5% Ixodid ticks (252 Ixodes scapularis, 161 A. americanum, 88 Rhipicephalus sanguineus, 50 Amblyomma maculatum, 19 Dermacentor variabilis, and 13 unidentified Amblyomma species produced a Borrelia flaB amplicon. Of the positive ticks, 324 (22.7% were collected from canines (151 A. americanum, 78 R. sanguineus, 43 I. scapularis, 26 A. maculatum, 18 D. variabilis, and 8 Amblyomma species and 259 (37.2% were collected from white-tailed deer (209 I. scapularis, 24 A. maculatum, 10 A. americanum, 10 R. sanguineus, 1 D. variabilis, and 5 Amblyomma species. None of the larvae were PCR positive. A majority of the flaB amplicons were homologous with B

  13. Human SCARB2-mediated entry and endocytosis of EV71.

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    Yi-Wen Lin

    Full Text Available Enterovirus (EV 71 infection is known to cause hand-foot-and-mouth disease (HFMD and in severe cases, induces neurological disorders culminating in fatality. An outbreak of EV71 in South East Asia in 1997 affected over 120,000 people and caused neurological disorders in a few individuals. The control of EV71 infection through public health interventions remains minimal and treatments are only symptomatic. Recently, human scavenger receptor class B, member 2 (SCARB2 has been reported to be a cellular receptor of EV71. We expressed human SCARB2 gene in NIH3T3 cells (3T3-SCARB2 to study the mechanisms of EV71 entry and infection. We demonstrated that human SCARB2 serves as a cellular receptor for EV71 entry. Disruption of expression of SCARB2 using siRNAs can interfere EV71 infection and subsequent inhibit the expression of viral capsid proteins in RD and 3T3-SCARB2 but not Vero cells. SiRNAs specific to clathrin or dynamin or chemical inhibitor of clathrin-mediated endocytosis were all capable of interfering with the entry of EV71 into 3T3-SCARB2 cells. On the other hand, caveolin specific siRNA or inhibitors of caveolae-mediated endocytosis had no effect, confirming that only clathrin-mediated pathway was involved in EV71 infection. Endocytosis of EV71 was also found to be pH-dependent requiring endosomal acidification and also required intact membrane cholesterol. In summary, the mechanism of EV71 entry through SCARB2 as the receptor for attachment, and its cellular entry is through a clathrin-mediated and pH-dependent endocytic pathway. This study on the receptor and endocytic mechanisms of EV71 infection is useful for the development of effective medications and prophylactic treatment against the enterovirus.

  14. Factors related to achievement in sophomore organic chemistry at the University of Arkansas

    Science.gov (United States)

    Lindsay, Harriet Arlene

    The purpose of this study was to identify the significant cognitive and non-cognitive variables that related to achievement in the first semester of organic chemistry at the University of Arkansas. Cognitive variables included second semester general chemistry grade, ACT composite score, ACT English, mathematics, reading, and science reasoning subscores, and spatial ability. Non-cognitive variables included anxiety, confidence, effectance motivation, and usefulness. Using a correlation research design, the individual relationships between organic chemistry achievement and each of the cognitive variables and non-cognitive variables were assessed. In addition, the relationships between organic chemistry achievement and combinations of these independent variables were explored. Finally, gender- and instructor-related differences in the relationships between organic chemistry achievement and the independent variables were investigated. The samples consisted of volunteers from the Fall 1999 and Fall 2000 sections of Organic Chemistry I at the University of Arkansas. All students in each section were asked to participate. Data for spatial ability and non-cognitive independent variables were collected using the Purdue Visualization of Rotations test and the modified Fennema-Sherman Attitude Scales. Data for other independent variables, including ACT scores and second semester general chemistry grades, were obtained from the Office of Institutional Research. The dependent variable, organic chemistry achievement, was measured by each student's accumulated points in the course and consisted of scores on quizzes and exams in the lecture section only. These totals were obtained from the lecture instructor at the end of each semester. Pearson correlation and stepwise multiple regression analyses were used to measure the relationships between organic chemistry achievement and the independent variables. Prior performance in chemistry as measured by second semester general

  15. Occurrence of organic wastewater and other contaminants in cave streams in northeastern Oklahoma and northwestern Arkansas

    Science.gov (United States)

    Bidwell, Joseph R.; Becker, C.; Hensley, S.; Stark, R.; Meyer, M.T.

    2010-01-01

    The prevalence of organic wastewater compounds in surface waters of the United States has been reported in a number of recent studies. In karstic areas, surface contaminants might be transported to groundwater and, ultimately, cave ecosystems, where they might impact resident biota. In this study, polar organic chemical integrative samplers (POCISs) and semipermeable membrane devices (SPMDs) were deployed in six caves and two surface-water sites located within the Ozark Plateau of northeastern Oklahoma and northwestern Arkansas in order to detect potential chemical contaminants in these systems. All caves sampled were known to contain populations of the threatened Ozark cavefish (Amblyopsis rosae). The surface-water site in Oklahoma was downstream from the outfall of a municipal wastewater treatment plant and a previous study indicated a hydrologic link between this stream and one of the caves. A total of 83 chemicals were detected in the POCIS and SPMD extracts from the surface-water and cave sites. Of these, 55 chemicals were detected in the caves. Regardless of the sampler used, more compounds were detected in the Oklahoma surface-water site than in the Arkansas site or the caves. The organic wastewater chemicals with the greatest mass measured in the sampler extracts included sterols (cholesterol and ??-sitosterol), plasticizers [diethylhexylphthalate and tris (2-butoxyethyl) phosphate], the herbicide bromacil, and the fragrance indole. Sampler extracts from most of the cave sites did not contain many wastewater contaminants, although extracts from samplers in the Oklahoma surfacewater site and the cave hydrologically linked to it had similar levels of diethylhexyphthalate and common detections of carbamazapine, sulfamethoxazole, benzophenone, N-diethyl-3-methylbenzamide (DEET), and octophenol monoethoxylate. Further evaluation of this system is warranted due to potential ongoing transport of wastewaterassociated chemicals into the cave. Halogenated organics

  16. Using Paid Radio Advertisements to Promote Physical Activity Among Arkansas Tweens

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    Appathurai Balamurugan, MD, MPH

    2005-11-01

    Full Text Available Introduction The level of physical activity among children is a growing concern. Evidence shows that many children aged 9 to 13 years (tweens do not participate in any organized physical activity during their nonschool hours, and some do not engage in any free-time physical activity. Physical inactivity is associated with a host of chronic diseases such as diabetes and cardiovascular disease. Paid media advertisements have been an effective method of promoting physical activity. Methods From March 10, 2003, through June 29, 2003, we aired paid radio advertisements in six major Arkansas metropolitan areas to promote physical activity among tweens. In September 2003, we surveyed 295 Arkansas tweens by telephone to assess their exposure to the advertisements and the impact of the advertisements on their intent to participate in physical activity. In the same telephone survey, we also asked questions about the respondents’ physical activity level. The data were weighted so that the results would be representative of the areas surveyed. The statistical analysis was performed using SPSS, version 11.5 (SPSS Inc, Chicago, Ill. Results Of the tweens surveyed, 56.4% (95% confidence interval [CI], 50.7%–62.1% reported hearing the radio advertisements. Of the tweens who heard the advertisement messages, 76.1% (95% CI, 69.4%–82.8% said the messages made them more likely to get involved in physical activity. Younger tweens (aged 9 and 10 years were less likely to have heard the advertisements than older tweens (aged 11 to 13 years. However, the advertisements were more likely to cause younger tweens to want to get involved in physical activity (odds ratio [OR] = 6.89, P = .003 than older tweens. Of the tweens surveyed, 74.9% (95% CI, 70.0%–79.8% reported that they were involved in nonschool-sponsored sports, and 45.3% (95% CI, 39.6%–51.0% were involved in school-sponsored sports. Conclusion Paid media advertisements may be an effective way to

  17. Development of EV71 virus-like particle purification processes.

    Science.gov (United States)

    Lin, Shih-Yeh; Chiu, Hsin-Yi; Chiang, Bor-Luen; Hu, Yu-Chen

    2015-11-01

    Enterovirus 71 (EV71) causes the outbreaks of hand-foot-and-mouth disease and results in deaths of hundreds of young children. EV71 virus-like particles (VLPs) are empty capsids consisting of viral structural proteins and can elicit potent immune responses, thus holding promise as an EV71 vaccine candidate. However, an efficient, scalable production and purification scheme is missing. For mass production of EV71 VLPs, this study aimed to develop a production and chromatography-based purification process. We first demonstrated the successful EV71 VLPs production in the stirred-tank bioreactor in which High Five™ cells were infected with a recombinant baculovirus co-expressing EV71 structural polyprotein P1 and protease 3CD. The culture supernatant containing the VLPs was subjected to tangential flow filtration (TFF) for concentration/diafiltration, which enabled the removal of >80% of proteins while recovering >80% of VLPs. The concentrated VLPs were next subjected to hydroxyapatite chromatography (HAC) in which the VLPs were mainly found in the flow through. After another TFF concentration/diafiltration, the VLPs were purified by size-exclusion chromatography (SEC) and concentrated/diafiltered by a final TFF. The integrated process yielded an overall VLPs recovery of ≈ 36% and a purity of ≈ 83%, which was better or comparable to the recovery and purity for the purification of live EV71 virus particles. This process thus may move the EV71 VLPs vaccine one step closer to the clinical applications.

  18. Animal models of enterovirus 71 infection: applications and limitations

    OpenAIRE

    Wang, Ya-Fang; Yu, Chun-Keung

    2014-01-01

    Human enterovirus 71 (EV71) has emerged as a neuroinvasive virus that is responsible for several outbreaks in the Asia-Pacific region over the past 15 years. Appropriate animal models are needed to understand EV71 neuropathogenesis better and to facilitate the development of effective vaccines and drugs. Non-human primate models have been used to characterize and evaluate the neurovirulence of EV71 after the early outbreaks in late 1990s. However, these models were not suitable for assessing ...

  19. 肠道病毒71型研究进展%STUDY ON THE PROGRESS OF ENTEROVIRUS 71

    Institute of Scientific and Technical Information of China (English)

    孙佳

    2011-01-01

    Hand, foot and mouth disease is an acute infectious disease caused by different enteroviruses. including the coxsackievirus A16 and hu-man enterovirus 71 etc. Since EV71 can cause severe central nervous system complications. This article reviewed the research progress on EV 71 , provided the basis for the prevention and treatment of HFMD.%手足口病是由不同的肠道病毒所引起的一种急性传染病,其中以柯萨奇病毒A16型和人肠道病毒EV71最为常见.由于EV71能引起严重的中枢神经系统并发症,本文就EV71的研究进展作一综述,为手足口病的预防和治疗提供依据.

  20. EV71疫苗研究进展%Progress in the research of EV71 vaccine

    Institute of Scientific and Technical Information of China (English)

    蔡宏荣; 王蕾; 廖芸

    2009-01-01

    Enterovirus 71 (EV71) is a major etiological agent of hand-foot-and-mouth disease (HFMD). Since no effeetive antiviral agents are available,developing effective EV71 vaccines for prevention of HFMD has been the control measure of choice. Currently, the research of EV71 vaccines has made certain pro-gress,and various EV71 vaccines, including attenuated vccines, inactivated vaccine, VP1 subunit vaccine and DNA vaccine, are being developed. Although various EV71 vaccines have certain efficacy, the optimal vaccine needs to be identified by evaluating the safety and protective efficacy of various EV71 vaccines.%肠道病毒71型(enterovims 71,EV71)是手足口病的主要病原体,由于尚无治疗手足口病的有效抗病毒药,因此,开发预防手足口病的有效EV71疫苗成为所有控制措施的首选.目前,EV71疫苗研究已取得一定的进展,正在研发的疫苗包括减毒或灭活疫苗、VP1亚单位疫苗和DNA疫苗.虽然各种疫苗均具有一定的免疫效果,但最适疫苗的确认还需对各种疫苗的安全性和保护效力进行评估.

  1. The interplays between autophagy and apoptosis induced by enterovirus 71.

    Directory of Open Access Journals (Sweden)

    Xueyan Xi

    Full Text Available BACKGROUND: Enterovirus 71 (EV71 is the causative agent of human diseases with distinct severity, from mild hand, foot and mouth disease to severe neurological syndromes, such as encephalitis and meningitis. The lack of understanding of viral pathogenesis as well as lack of efficient vaccine and drugs against this virus impedes the control of EV71 infection. EV71 virus induces autophagy and apoptosis; however, the relationship between EV71-induced autophagy and apoptosis as well as the influence of autophagy and apoptosis on virus virulence remains unclear. METHODOLOGY/PRINCIPAL FINDINGS: In this study, it was observed that the Anhui strain of EV71 induced autophagy and apoptosis in human rhabdomyosarcoma (RD-A cells. Additionally, by either applying chemical inhibitors or knocking down single essential autophagic or apoptotic genes, inhibition of EV71 induced autophagy inhibited the apoptosis both at the autophagosome formation stage and autophagy execution stage. However, inhibition of autophagy at the stage of autophagosome and lysosome fusion promoted apoptosis. In reverse, the inhibition of EV71-induced apoptosis contributed to the conversion of microtubule-associated protein 1 light chain 3-I (LC3-I to LC3-II and degradation of sequestosome 1 (SQSTM1/P62. Furthermore, the inhibition of autophagy in the autophagsome formation stage or apoptosis decreased the release of EV71 viral particles. CONCLUSIONS/SIGNIFICANCE: In conclusion, the results of this study not only revealed novel aspect of the interplay between autophagy and apoptosis in EV71 infection, but also provided a new insight to control EV71 infection.

  2. Progress in research of enterovirus 71 vaccines%EV71疫苗研制新进展

    Institute of Scientific and Technical Information of China (English)

    赵蕾; 陈敬贤; 王明丽

    2013-01-01

    肠道病毒71型(enterovirus 71,EV71)是引起手足口病和神经系统并发症的重要病原体.近几年,世界多个地区发生了由EV72感染引起的手足口病.由于目前尚无有效的抗病毒药物对患者进行治疗,因而疫苗的研制成为预防和控制EV71传播的关键.此文主要就近年EV71疫苗的研制进展做一综述.%Enterovirus 71 (EV71) is known to be a causative agent of hand,foot and mouth disease (HFMD) and severe neurological complications.Many outbreaks caused by EV71 have occurred in different regions of the world.Due to lacking an effective antiviral agent,development of effective vaccines has become the top priority in terms of control strategies.This article reviews the recent advancement in the design of various kinds of vaccines against EV71.

  3. Enterovirus71 (EV71) utilise host microRNAs to mediate host immune system enhancing survival during infection.

    Science.gov (United States)

    Lui, Yan Long Edmund; Tan, Tuan Lin; Woo, Wee Hong; Timms, Peter; Hafner, Louise Marie; Tan, Kian Hwa; Tan, Eng Lee

    2014-01-01

    Hand, Foot and Mouth Disease (HFMD) is a self-limiting viral disease that mainly affects infants and children. In contrast with other HFMD causing enteroviruses, Enterovirus71 (EV71) has commonly been associated with severe clinical manifestation leading to death. Currently, due to a lack in understanding of EV71 pathogenesis, there is no antiviral therapeutics for the treatment of HFMD patients. Therefore the need to better understand the mechanism of EV71 pathogenesis is warranted. We have previously reported a human colorectal adenocarcinoma cell line (HT29) based model to study the pathogenesis of EV71. Using this system, we showed that knockdown of DGCR8, an essential cofactor for microRNAs biogenesis resulted in a reduction of EV71 replication. We also demonstrated that there are miRNAs changes during EV71 pathogenesis and EV71 utilise host miRNAs to attenuate antiviral pathways during infection. Together, data from this study provide critical information on the role of miRNAs during EV71 infection.

  4. The UP modelling system for large scale hydrology: simulation of the Arkansas-Red River basin

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    C. G. Kilsby

    1999-01-01

    Full Text Available The UP (Upscaled Physically-based hydrological modelling system to the Arkansas-Red River basin (USA is designed for macro-scale simulations of land surface processes, and aims for a physical basis and, avoids the use of discharge records in the direct calibration of parameters. This is achieved in a two stage process: in the first stage parametrizations are derived from detailed modelling of selected representative small and then used in a second stage in which a simple distributed model is used to simulate the dynamic behaviour of the whole basin. The first stage of the process is described in a companion paper (Ewen et al., this issue, and the second stage of this process is described here. The model operated at an hourly time-step on 17-km grid squares for a two year simulation period, and represents all the important hydrological processes including regional aquifer recharge, groundwater discharge, infiltration- and saturation-excess runoff, evapotranspiration, snowmelt, overland and channel flow. Outputs from the model are discussed, and include river discharge at gauging stations and space-time fields of evaporation and soil moisture. Whilst the model efficiency assessed by comparison of simulated and observed discharge records is not as good as could be achieved with a model calibrated against discharge, there are considerable advantages in retaining a physical basis in applications to ungauged river basins and assessments of impacts of land use or climate change.

  5. Predictors of Substance Abuse Treatment Entry Among Rural Illicit Stimulant Users in Ohio, Arkansas, and Kentucky

    Science.gov (United States)

    Carlson, Robert G.; Sexton, Rocky; Wang, Jichuan; Falck, Russel; Leukefeld, Carl G.; Booth, Brenda M.

    2010-01-01

    Illicit drug use in the rural United States is increasingly common, yet little is known about drug users’ treatment-seeking behaviors. This study identifies predictors of substance abuse treatment entry over 24 months among 710 illicit stimulant users in rural areas of Ohio, Arkansas, and Kentucky. Active users of powdered cocaine, crack cocaine, and/or methamphetamine (MA) were recruited using respondent-driven sampling. Participants completed structured interviews at baseline and follow-up questionnaires every 6 months for 24 months. Data were analyzed using the Cox proportional hazards model. The paper is informed by the Anderson-Newman Model. Overall, 18.7% of the sample entered treatment. Ohio or Kentucky residence, perceived need for substance abuse treatment, higher ASI legal problem composite scores, prior substance abuse treatment, and tranquilizer use were positively associated with treatment entry. Non-daily crack cocaine users and marijuana users were less likely to enter treatment. The findings can help inform rural substance abuse treatment program development and outreach. PMID:20391264

  6. Digital model of the Bayou Bartholomew alluvial aquifer stream system, Arkansas

    Science.gov (United States)

    Reed, J.E.; Broom, Matthew E.

    1979-01-01

    A digital model of the Bayou Bartholomew aquifer-stream system in Arkansas was calibrated for the purpose of predicting hydrologic responses to stresses of water development. The simulated-time span for model calibration was from 1953 to 1970, during which time the system was stressed largely by ground- and surface-water diversions for rice irrigation. The model was calibrated by comparing groundwater-level and streamflow data with model-derived groundwater levels and streamflow. In the calibrated model, the ratio of model-derived to observed streamflows for 17 subbasins averaged 1.1; the ratios among the subbasins ranged from 0.8 to 1.6. The average deviation of the differences between model-derived and observed groundwater levels at 47 nodes was 0.2; the average among the nodes ranged from -2.3 to 10.4. The average standard deviation of the differences between the model-derived and observed groundwater levels was 3.5; the average among the nodes ranged from 0.4 to 10.5. The model will provide projections of changes in the potentiometric surface resulting from (1) changes in the rate or distribution of groundwater pumpage or (2) changes in the stage of streams and reservoirs. The model will provide only approximate projections of the streamflow. (USGS)

  7. Bacteria and Turbidity Survey for Blue Mountain Lake, Arkansas, Spring and Summer, 1994

    Science.gov (United States)

    Lasker, A. Dwight

    1995-01-01

    Introduction Blue Mountain Lake darn is located at river mile 74.4 on the Petit Jean River in Logan and Yell Counties in west-central Arkansas (fig. 1). Drainage area above the darn is 488 square miles. Blue Mountain Lake is located between two national forests-the Ozark National Forest and the Ouachita National Forest. The primary purpose for Blue Mountain Lake is flood control, but the lake is used for a variety of recreational purposes. The U.S. Geological Survey (USGS) in cooperation with the U.s. Army Corps of Engineers, Little Rock District, conducted a bacterial and turbidity study of the Blue Mountain Lake Basin during the spring and suri1mer 1994. Samples were collected weekly at 11 locations within the lake basin from May through September 1994. Eight sampling sites were located on tributaries to the lake and three sampling sites were located on the lake with one of the sites located at a swim beach (fig. 2; table 1).

  8. Size distribution of planktonic autotrophy and microheterotrophy in DeGray Reservoir, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Kimmel, B.L.; Groeger, A.W.

    1983-01-01

    Naturally occurring assemblages of phytoplankton and bacterioplankton were radiolabelled with sodium /sup 14/C-bicarbonate and sodium /sup 3/H-acetate and size fractionated to determine the size structure of planktonic autotrophy and microheterotrophy in DeGray Reservoir, an oligotrophic impoundment of the Caddo River in south-central Arkansas. Size distributions of autotrophy and microheterotrophy were remarkably uniform seasonally, vertically within the water column, and along the longitudinal axis of the reservoir despite significant changes in environmental conditions. Planktonic autotrophy was dominated by small algal cells with usually >50% of the photosynthetic carbon uptake accounted for by organisms <8.0 ..mu..m. Microheterotrophic activity in the 0.2- to 1.0-..mu..m size fraction, presumably associated with free-living bacterioplankton not attached to suspended particles, usually accounted for >75% of the planktonic microheterotrophy. Longitudinal patterns in autotrophic and microheterotrophic activities associated with >3-..mu..m and >1-..mu..m size fractions, respectively, suggest an uplake to downlake shift from riverine to lacustrine environmental influences within the reservoir. 83 references, 7 figures.

  9. Duplex-like structures in submarine fan channels, Ouachita Mountains, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Shanmugam, G.; Moiola, R.J.; Sales, J.K.

    1988-03-01

    Submarine fan channel sequences of the Jackfork Formation (Lower Pennsylvanian) at DeGray Dam section in the Ouachita Mountains, Arkansas, contain discrete units (10-75 cm thick) with moderately dipping (25/sup 0/-40/sup 0/), sigmoidal imbricate slices. Adjacent units with opposing imbricate slices are common. The sigmoidal structures are similar in geometry to a tectonic feature known as a duplex. A tectonic origin of sigmoidal structures, however, seems unlikely because opposing directions of imbrication in adjacent units would require an unrealistic tectonic movement history for the area. The authors propose that the Jackfork sigmoidal structures were formed by a process kinematically similar to that responsible for generating duplex structures. Unlike tectonic duplexes, however, the sigmoidal structures were formed by soft-sediment deformation of sand and mud layers as high-energy sediment gravity flows glided over these layers. Sediment gravity flows, responsible for forming the sigmoidal deformation, were probably generated by slumping of adjacent channel walls. Dip direction of sigmoidal slices is perpendicular to channel axes. Thus, recognition of sigmoidal deformation structures may be useful in inferring the trend of channels in ancient submarine fan complexes.

  10. Atmospheric Contributors to Heavy Rainfall Events in the Arkansas-Red River Basin

    Directory of Open Access Journals (Sweden)

    Taylor A. McCorkle

    2016-01-01

    Full Text Available This study analyzed the top 1% 24-hour rainfall events from 1994 to 2013 at eight climatological sites that represent the east to west precipitation gradient across the Arkansas-Red River Basin in North America. A total of 131 cases were identified and subsequently classified on the synoptic-scale, mesoscale, and local-scale to compile a climatological analysis of these extreme, heavy rainfall events based on atmospheric forcings. For each location, the prominent midtropospheric pattern, mesoscale feature, and predetermined thermodynamic variables were used to classify each 1% rainfall event. Individual events were then compared with other cases throughout the basin. The most profound results were that the magnitudes of the thermodynamic variables such as convective available potential energy and precipitable water values were poor predictors of the amount of rainfall produced in these extreme events. Further, the mesoscale forcings had more of an impact during the warm season and for the westernmost locations, whereas synoptic forcings were extremely prevalent during the cold season at the easternmost locations in the basin. The implications of this research are aimed at improving the forecasting of heavy precipitation at individual weather forecasts offices within the basin through the identified patterns at various scales.

  11. Disposal of chemical agents and munitions stored at Pine Bluff Arsenal, Pine Bluff, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Ensminger, J.T.; Hillsman, E.L.; Johnson, R.D.; Morrisey, J.A.; Staub, W.P.; Boston, C.R.; Hunsaker, D.B.; Leibsch, E.; Rickert, L.W.; Tolbert, V.R.; Zimmerman, G.P.

    1991-09-01

    The Pine Bluff Arsenal (PBA) near Pine Bluff, Arkansas, is one of eight continental United States (CONUS) Army installations where lethal unitary chemical agents and munitions are stored and where destruction of agents and munitions is proposed under the Chemical Stockpile Disposal Program (CSDP). The chemical agent inventory at PBA consists of approximately 12%, by weight, of the total US stockpile. The destruction of the stockpile is necessary to eliminate the risk to the public from continued storage and to dispose of obsolete and leaking munitions. In 1988 the US Army issued a Final Programmatic Environmental Impact Statement (FPEIS) for the CSDP that identified on-site disposal of agents and munitions as the environmentally preferred alternative (i.e., the alternative with the least potential to cause significant adverse impacts). The purpose of this report is to examine the proposed implementation of on-site disposal at PBA in light of more recent and more detailed data than those on which the FPEIS is based. New population data were used to compute fatalities using the same computation methods and values for all other parameters as in the FPEIS. Results indicate that all alternatives are indistinguishable when the potential health impacts to the PBA community are considered. However, risks from on-site disposal are in all cases equal to or less than risks from other alternatives. Furthermore, no unique resources with the potential to prevent or delay implementation of on-site disposal at PBA have been identified.

  12. Cadmium Accumulation in Periphyton from an Abandoned Mining District in the Buffalo National River, Arkansas.

    Science.gov (United States)

    McCauley, Jacob R; Bouldin, Jennifer L

    2016-06-01

    The Rush Mining District along the Buffalo River in Arkansas has a significant history of zinc and lead mining operations. The tails and spoils of these operations deposit heavy amounts of raw ore into streams. One element commonly found in the earth's crust that becomes a minor constituent of the deposition is cadmium. Periphyton samples from Rush Creek and Clabber Creek, two creeks within the Rush Mining District were measured for cadmium as well as two creeks with no history of mining, Spring Creek and Water Creek. Periphyton samples from Rush and Clabber Creek contained mean cadmium concentrations of 436.6 ± 67.3 and 93.38 ± 8.67 µg/kg, respectively. Spring Creek and Water Creek had a mean cadmium concentration of 40.49 ± 3.40 and 41.78 ± 3.99 µg/kg within periphyton. The results indicate increased metal concentrations in algal communities from mined areas. As periphyton is the base of the aquatic food chain, it acts as a conduit for movement of cadmium in the food web. PMID:27130541

  13. Detection of Lead (Pb) in Three Environmental Matrices of the Cache River Watershed, Arkansas.

    Science.gov (United States)

    Kilmer, Mary K; Bouldin, Jennifer L

    2016-06-01

    Water bodies contaminated with lead (Pb) represent a considerable threat to both human and environmental health. The Cache River, located in northeastern Arkansas has been listed as impaired on the 303(d) list due to Pb contamination. However, historical data for the watershed is limited in both sampled waterways and analyses performed. This study measures concentrations of Pb in three environmental matrices of the Cache River Watershed; dissolved in the water column, total Pb (dissolved + particulate), and sediment-bound Pb. A variety of waterways were sampled including main channel and tributary sites. Frequency of detection and mean concentrations were compared to values for the entire Lower Mississippi Watershed. In general, no significant differences were found for the CRW when compared to the LMRW, with the exception of total Pb which was detected more frequently but at lower concentrations in the CRW than in the LMRW, and sediment Pb, which was detected at a significantly lower frequency in the CRW than the LMRW. PMID:26858084

  14. Hard lives, God's help, and struggling through: caregiving in Arkansas Delta.

    Science.gov (United States)

    Gerdner, Linda A; Tripp-Reimer, Toni; Simpson, Helen C

    2007-12-01

    The purpose of this ethnographic study was to describe the experience of African American adults providing in-home care for a family member with chronic confusion living in the Arkansas Delta. We conducted this study over fourteen months in two rural Delta counties using participant observation and in-depth interviews. The majority of caregivers were adult daughters. Nearly half attributed chronic confusion to a difficult life and emotional stress or "worry;" a third believed it to be a natural component of "old age." Caregivers tended to view their work as an expression of love and devotion that was accompanied by emotional stress and personal sacrifice. While just under half of the caregivers had no other family members willing or available to assist with the physical care of the elder, the majority were able to turn to family members for emotional support. Further they identified strong religious beliefs as the primary force that sustained them in the caregiving role. The centrality of spirituality, faith/religion was noted in nearly all aspects of life. Faith in God was seen as continuing to be an important aspect of the care recipients' lives as well. Recipients' spiritual needs were addressed by accompanying the care recipient to church services and reading Bible passages to them on a regular basis. Selective community services (i.e., adult day care, home health services) were used that supported efforts at maintaining the family caregiver role. Findings are discussed within the context of historical and sociopolitical factors of the geographic region.

  15. Description of Survey Data Regarding the Chemical Repackaging Plant Accident West Helena, Arkansas

    Energy Technology Data Exchange (ETDEWEB)

    Sorensen, J.H.; Vogt, B.M.

    1999-03-01

    Shortly after 1:00 p.m. on Thursday, May 8, 1997, clouds of foul-smelling smoke began pouring from an herbicide and pesticide packaging plant in West Helena, Arkansas. An alert was sounded, employees evacuated, and the West Helena fire department was called. As three firefighters prepared to enter the plant, the chemical compounds exploded, collapsing a solid concrete block wall, and killing all three firefighters. As the odorous smoky cloud drifted away from the plant, authorities ordered residents in a 2-mile area downwind of the plant to evacuate and those in the 2- to 3-mile zone to shelter in place. This study examines and compares the responses to a mail survey of those ordered to evacuate and those told to shelter in place. Among the variables examined are compliance with official orders and perceived warnings, threat perception, time and source of first warning, response times, and behavior characteristics for both populations. The findings indicate that 90% of those that were told to evacuate did so but only 27% of those told to shelter-in-place did so, with 68% opting to evacuate instead. The implications of these findings for emergency managers is that people will likely choose to evacuate when both warnings to evacuate and warnings to shelter are issued to residents in close proximity to each other. The findings on warning times closely resemble other findings from evacuations when chemical accidents occur and route notification is used for warning residents.

  16. 48 CFR 352.239-71 - Standard for encryption language.

    Science.gov (United States)

    2010-10-01

    ... language. 352.239-71 Section 352.239-71 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES... Standard for encryption language. As prescribed in 339.101(d)(2), the Contracting Officer shall insert the following clause: Standard for Encryption Language (January 2010) (a) The Contractor shall use...

  17. 29 CFR 458.71 - Procedure upon admission of facts.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 2 2010-07-01 2010-07-01 false Procedure upon admission of facts. 458.71 Section 458.71... facts. The admission of all the material allegations of fact in the complaint shall constitute a waiver... his recommended decision and order in which he shall adopt as his proposed findings of fact...

  18. 27 CFR 71.65 - Answer admitting facts.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Answer admitting facts. 71... Hearing Procedure Answers § 71.65 Answer admitting facts. If the respondent desires to waive the hearing on the allegations of fact set forth in the order to show cause, and does not contest the facts,...

  19. 48 CFR 1852.228-71 - Aircraft flight risks.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Aircraft flight risks. 1852.228-71 Section 1852.228-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND SPACE... the aircraft has returned to the ground and rotors are disengaged. (iv) With respect to vertical...

  20. 42 CFR 71.44 - Disinsection of aircraft.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Disinsection of aircraft. 71.44 Section 71.44... Disinsection of aircraft. (a) The Director may require disinsection of an aircraft if it has left a foreign area that is infected with insect-borne communicable disease and the aircraft is suspected of...

  1. 48 CFR 552.241-71 - Disputes (Utility Contracts).

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Disputes (Utility....241-71 Disputes (Utility Contracts). As prescribed in 541.501, insert clause 552.241-71, Disputes (Utility Contracts), in solicitations and contracts for utility services subject to the jurisdiction...

  2. 40 CFR 164.71 - Fees of witnesses.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Fees of witnesses. 164.71 Section 164.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS RULES OF PRACTICE GOVERNING HEARINGS, UNDER THE FEDERAL INSECTICIDE, FUNGICIDE, AND RODENTICIDE ACT, ARISING...

  3. 50 CFR 216.71 - Allowable take of fur seals.

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b)...

  4. 14 CFR 23.71 - Glide: Single-engine airplanes.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Glide: Single-engine airplanes. 23.71... AIRWORTHINESS STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Performance § 23.71 Glide: Single-engine airplanes. The maximum horizontal distance traveled in still air, in nautical...

  5. 27 CFR 21.71 - Formula No. 39-B.

    Science.gov (United States)

    2010-04-01

    ... and one-half gallons of diethyl phthalate and 1/8 gallon of tert-butyl alcohol. (b) Authorized uses... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Formula No. 39-B. 21.71 Section 21.71 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU,...

  6. 34 CFR 668.71 - Scope and special definitions.

    Science.gov (United States)

    2010-07-01

    ... substantial misrepresentation made by that institution regarding the nature of its educational program, its... 34 Education 3 2010-07-01 2010-07-01 false Scope and special definitions. 668.71 Section 668.71... and special definitions. (a) This subpart establishes the standards and rules by which the...

  7. 27 CFR 71.5 - Meaning of terms.

    Science.gov (United States)

    2010-04-01

    ... Section 71.5 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... Definitions § 71.5 Meaning of terms. When used in this part and in forms prescribed under this part, where not... under this part. Administrator. The Administrator, Alcohol and Tobacco Tax and Trade Bureau,...

  8. Anti-Enterovirus 71 Agents of Natural Products.

    Science.gov (United States)

    Wang, Liyan; Wang, Junfeng; Wang, Lishu; Ma, Shurong; Liu, Yonghong

    2015-01-01

    This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005-2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded. PMID:26370955

  9. 32 CFR 644.71 - Final Title Assembly.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Final Title Assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... Title Assembly. (a) Disposition of final title assemblies. The final title opinion and related...

  10. 41 CFR 105-71.141 - Financial reporting.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Financial reporting. 105... GOVERNMENTS 71.14-Post-Award Requirements/Reports, Records, Retention, and Enforcement § 105-71.141 Financial reporting. (a) General. (1) Except as provided in paragraphs (a) (2) and (5) of this section, grantees...

  11. 7 CFR 1212.71 - Books and records.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Books and records. 1212.71 Section 1212.71 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HONEY PACKERS AND...

  12. 7 CFR 984.71 - Reports of handler inventory.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Reports of handler inventory. 984.71 Section 984.71 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE WALNUTS GROWN IN CALIFORNIA...

  13. 42 CFR 71.54 - Etiological agents, hosts, and vectors.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Etiological agents, hosts, and vectors. 71.54..., INSPECTION, LICENSING FOREIGN QUARANTINE Importations § 71.54 Etiological agents, hosts, and vectors. (a) A... any arthropod or other animal host or vector of human disease, or any exotic living arthropod or...

  14. 27 CFR 4.71 - Standard wine containers.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Standard wine containers. 4.71 Section 4.71 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS LABELING AND ADVERTISING OF WINE Standards of Fill for Wine §...

  15. Anti-Enterovirus 71 Agents of Natural Products

    Directory of Open Access Journals (Sweden)

    Liyan Wang

    2015-09-01

    Full Text Available This review, with 42 references, presents the fascinating area of anti-enterovirus 71 natural products over the last three decades for the first time. It covers literature published from 2005–2015 and refers to compounds isolated from biogenic sources. In total, 58 naturally-occurring anti-EV71 compounds are recorded.

  16. An adenosine nucleoside analogue NITD008 inhibits EV71 proliferation.

    Science.gov (United States)

    Shang, Luqing; Wang, Yaxin; Qing, Jie; Shu, Bo; Cao, Lin; Lou, Zhiyong; Gong, Peng; Sun, Yuna; Yin, Zheng

    2014-12-01

    Enterovirus 71 (EV71), one of the major causative agents of Hand-Foot-Mouth Disease (HFMD), causes severe pandemics and hundreds of deaths in the Asia-Pacific region annually and is an enormous public health threat. However, effective therapeutic antiviral drugs against EV71 are rare. Nucleoside analogues have been successfully used in the clinic for the treatment of various viral infections. We evaluated a total of 27 nucleoside analogues and discovered that an adenosine nucleoside analogue NITD008, which has been reported to be an antiviral reagent that specifically inhibits flaviviruses, effectively suppressed the propagation of different strains of EV71 in RD, 293T and Vero cells with a relatively high selectivity index. Triphosphorylated NITD008 (ppp-NITD008) functions as a chain terminator to directly inhibit the RNA-dependent RNA polymerase activity of EV71, and it does not affect the EV71 VPg uridylylation process. A significant synergistic anti-EV71 effect of NITD008 with rupintrivir (AG7088) (a protease inhibitor) was documented, supporting the potential combination therapy of NITD008 with other inhibitors for the treatment of EV71 infections.

  17. The complete genome of Enterovirus 71 China strain

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Five overlapping clones covering the full genome of Enterovirus71 China strain SHZH98 were obtained and then the sequences were determined by the chain termination method. It showed that the full length of EV71 SHZH98 genome (not including Poly A tail) is 7408 bp. There are some diversities on the lengths and sequences of 5′ UTR and 3′ UTR between SHZH98 and the other EV71 strains. In P1 capsid region, which is closely associated with viral immunogenicity, EV71 strain SHZH98 shares the highest homology with Taiwan strains; but in P2 and P3 non-structural gene regions there are higher identities with Coxsakievirus A16 and EV71 strains MS, BrCr than with Taiwan strains. Phylogenetic tree constructed by structural gene region indicates that China strain SHZH98 has a closer relationship with Taiwan strains, however, in the non-coding region it has a closer relationship with Coxsakievirus A16, EV71 strains MS and BrCr. EV71 China strain was analyzed at the molecular level. The results will contribute to the basic study on enteroviruses and the EV71 prevention in China.

  18. 21 CFR 352.71 - Light source (solar simulator).

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Light source (solar simulator). 352.71 Section 352.71 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... Light source (solar simulator). A solar simulator used for determining the SPF of a sunscreen...

  19. 48 CFR 2452.239-71 - Information Technology Virus Security.

    Science.gov (United States)

    2010-10-01

    ... computer viruses. The contractor shall be responsible for examining all such products prior to their... resulting from computer virus damage or malicious destruction of computer information arising from the... Virus Security. 2452.239-71 Section 2452.239-71 Federal Acquisition Regulations System DEPARTMENT...

  20. 48 CFR 1452.237-71 - Utilization of Woody Biomass.

    Science.gov (United States)

    2010-10-01

    ... Biomass. 1452.237-71 Section 1452.237-71 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... Utilization of Woody Biomass. As prescribed in § 1437.7202, insert the following clause: Utilization of Woody Biomass (MAY 2005) (a) The contractor may remove and utilize woody biomass, if: (1) Project work...

  1. Animal models of enterovirus 71 infection: applications and limitations.

    Science.gov (United States)

    Wang, Ya-Fang; Yu, Chun-Keung

    2014-01-01

    Human enterovirus 71 (EV71) has emerged as a neuroinvasive virus that is responsible for several outbreaks in the Asia-Pacific region over the past 15 years. Appropriate animal models are needed to understand EV71 neuropathogenesis better and to facilitate the development of effective vaccines and drugs. Non-human primate models have been used to characterize and evaluate the neurovirulence of EV71 after the early outbreaks in late 1990s. However, these models were not suitable for assessing the neurovirulence level of the virus and were associated with ethical and economic difficulties in terms of broad application. Several strategies have been applied to develop mouse models of EV71 infection, including strategies that employ virus adaption and immunodeficient hosts. Although these mouse models do not closely mimic human disease, they have been applied to determine the pathogenesis of and treatment and prevention of the disease. EV71 receptor-transgenic mouse models have recently been developed and have significantly advanced our understanding of the biological features of the virus and the host-parasite interactions. Overall, each of these models has advantages and disadvantages, and these models are differentially suited for studies of EV71 pathogenesis and/or the pre-clinical testing of antiviral drugs and vaccines. In this paper, we review the characteristics, applications and limitation of these EV71 animal models, including non-human primate and mouse models. PMID:24742252

  2. 48 CFR 2452.209-71 - Limitation on future contracts.

    Science.gov (United States)

    2010-10-01

    ... or through the performance of tasks pursuant to this contract, is required to develop specifications... contracts. 2452.209-71 Section 2452.209-71 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions...

  3. 48 CFR 1852.231-71 - Determination of compensation reasonableness.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Determination of compensation reasonableness. 1852.231-71 Section 1852.231-71 Federal Acquisition Regulations System NATIONAL... Contract Act employees proposed that do not fail within the scope of any classification listed in...

  4. 48 CFR 2115.404-71 - Profit analysis factors.

    Science.gov (United States)

    2010-10-01

    .... 2115.404-71 Section 2115.404-71 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT... the Contractor's risk annually in relation to the amount in the Employees' Life Insurance Fund and... of a unique and enhanced customer support system, that are of demonstrated value to the FEGLI...

  5. 10 CFR 71.63 - Special requirement for plutonium shipments.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Special requirement for plutonium shipments. 71.63 Section... MATERIAL Package Approval Standards § 71.63 Special requirement for plutonium shipments. Shipments containing plutonium must be made with the contents in solid form, if the contents contain greater than...

  6. 10 CFR 71.88 - Air transport of plutonium.

    Science.gov (United States)

    2010-01-01

    ... citation of 49 CFR chapter I, as may be applicable, the licensee shall assure that plutonium in any form... carrier, require compliance with 49 CFR 175.704, U.S. Department of Transportation regulations applicable... 10 Energy 2 2010-01-01 2010-01-01 false Air transport of plutonium. 71.88 Section 71.88...

  7. 50 CFR 27.71 - Motion or sound pictures.

    Science.gov (United States)

    2010-10-01

    ... provisions of 43 CFR part 5. ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Motion or sound pictures. 27.71 Section 27... Equipment § 27.71 Motion or sound pictures. The taking or filming of any motion or sound pictures on...

  8. Complete Genome Sequence of Human Enterovirus Strain 71 (EV71/Taipei/3118/2011), Isolated from a Patient in Taiwan.

    Science.gov (United States)

    Lin, Chia-Pei; Liu, Jiung-Liang; Chen, Lung-Yuan; Liu, Yi-Chao; Wang, Hsiu-Chi; Lin, Shih-Jie; Chen, Pin-Chun; Wang, Kun-Teng; Huang, Chih-Hung; Yang, Yi-Chan; Cheng, Hwei-Fang; Shih, Daniel Yang-Chih; Wang, Der-Yuan

    2015-01-01

    This full-length genome sequence of human enterovirus strain 71 (EV71/Taipei/3118/2011) was isolated from a clinical patient in Taiwan in 2011. According to the phylogenetic analysis, the complete genome sequence in this study is part of the subgenotype C4.

  9. EV71疫苗研究进展%Progress of reaseach on vaccine of Enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    刘刚; 姚昕; 李凤祥

    2009-01-01

    肠道病毒71型(EV71),可引起手足口病(HFMD)和严重神经系统疾病.近年来,在世界多个国家和地区报道了EV71的爆发.由于目前缺乏有效的抗病毒药物,因此研制有效的疫苗是控制EV71流行最为有效的策略.近年来很多报道显示在EV71疫苗研究上取得了重要进展,本文以此为重点概括了EV71灭活疫苗、重组疫苗、DNA疫苗、多肽疫苗、减毒活疫苗等以及动物模型方面的研究进展.%Enterovirus 71(EV71)is known to be a causative agent of hand-foot-mouth disease(HFMD)and severe neurological complications.Many Ev71 outbreaks have been reposed in different regions of the world.Due to the lack of an effective antiviralagent,primary prevention of the disease,including the development of effective vaccines,has been the top priority in terms of control strategies.Recently,many reports indicated lots of important progression is acquired.In this article,we renewed the progression of EV71 animal model and vaccines which inchded inactivation vaccine,recombinant vaccine,DNA vaccine,polypeptide vaccine and live attenuated vaccine.

  10. The complete genome of Enterovirus 71 China strain

    Institute of Scientific and Technical Information of China (English)

    YANG; Fan

    2001-01-01

    [1]Jin Qi, Medical Molecular Virology (in Chinese), Beijing: Science Press, 2001.[2]McMinn, P., Stratov, I., Dowse, G., Enterovirus 71 outbreak in Western Australia associated with acute flaccid paralysis, Preliminary report, Commun. Dis. Intell., 1999, 23(7): 199.[3]Ho, M., Chen, E. R., Hsu, K. H. et al., An epidemic of Enterovirus 71 infection in Taiwan, The New England Journal of Medicine, 1999, 341(13): 929.[4]He Yaqing, Yang Fan, Li Chengliang et al., Identification of EV71 isolates from hand, foot and mouth disease cases in ShenZhen, Chinese Journal of Experimental and Clinical Virology (in Chinese), 1999, 1: 112.[5]Landry, M. L., Fonseca, S. N. S., Cohen, S. et al., Fatal enterovirus type 71 infection: rapid detection and diagnostic pitfalls, Pediatr. Infect. Dis. J., 1995, 14: 1095.[6]Brown, B. A., Pallansch, M. A., Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus, Virus Res., 1995, 39: 195.[7]Brown, B. A., Oberste, M. S., Alexander, J. P. Jr., Molecular epidemiology and evolution of Enterovirus 71 strains isolated from 1970 to 1998, J. Virol., 1999, 73(12): 9969.[8]Ishimaru,Y., Nakano,S., Yamaoda,K.et al.,Outbreaks of hand,foot,and mouth disease by Enterovirus 71,Arch.Dis.Child.,1980,55:583.

  11. Immunological Evaluation and Comparison of Different EV71 Vaccine Candidates

    Directory of Open Access Journals (Sweden)

    Ai-Hsiang Chou

    2012-01-01

    Full Text Available Enterovirus 71 (EV71 and coxsackievirus A16 (CVA16 are major causative agents of hand, foot, and mouth diseases (HFMDs, and EV71 is now recognized as an emerging neurotropic virus in Asia. Effective medications and/or prophylactic vaccines against HFMD are not available. The current results from mouse immunogenicity studies using in-house standardized RD cell virus neutralization assays indicate that (1 VP1 peptide (residues 211–225 formulated with Freund’s adjuvant (CFA/IFA elicited low virus neutralizing antibody response (1/32 titer; (2 recombinant virus-like particles produced from baculovirus formulated with CFA/IFA could elicit good virus neutralization titer (1/160; (3 individual recombinant EV71 antigens (VP1, VP2, and VP3 formulated with CFA/IFA, only VP1 elicited antibody response with 1/128 virus neutralization titer; and (4 the formalin-inactivated EV71 formulated in alum elicited antibodies that cross-neutralized different EV71 genotypes (1/640, but failed to neutralize CVA16. In contrast, rabbits antisera could cross-neutralize strongly against different genotypes of EV71 but weakly against CVA16, with average titers 1/6400 and 1/32, respectively. The VP1 amino acid sequence dissimilarity between CVA16 and EV71 could partially explain why mouse antibodies failed to cross-neutralize CVA16. Therefore, the best formulation for producing cost-effective HFMD vaccine is a combination of formalin-inactivated EV71 and CAV16 virions.

  12. Inhibition of enterovirus 71 entry by transcription factor XBP1

    Energy Technology Data Exchange (ETDEWEB)

    Jheng, Jia-Rong; Lin, Chiou-Yan [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China); Horng, Jim-Tong, E-mail: jimtong@mail.cgu.edu.tw [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China); Lau, Kean Seng [Department of Biochemistry and Research Center for Emerging Viral Infections, Chang Gung University, 259 Wen-Hwa First Road, Kweishan, Taoyuan 333, Taiwan (China)

    2012-04-20

    Highlights: Black-Right-Pointing-Pointer IRE1 was activated but no XBP1 splicing was detected during enterovirus 71 infection. Black-Right-Pointing-Pointer XBP1 was subject to translational shutoff by enterovirus 71-induced eIF4G cleavage. Black-Right-Pointing-Pointer The uptake of UV-irradiated virus was decreased in XBP1-overexpressing cells. -- Abstract: Inositol-requiring enzyme 1 (IRE1) plays an important role in the endoplasmic reticulum (ER), or unfolded protein, stress response by activating its downstream transcription factor X-box-binding protein 1 (XBP1). We demonstrated previously that enterovirus 71 (EV71) upregulated XBP1 mRNA levels but did not activate spliced XBP1 (XBP1s) mRNA or its downstream target genes, EDEM and chaperones. In this study, we investigated further this regulatory mechanism and found that IRE1 was phosphorylated and activated after EV71 infection, whereas its downstream XBP1s protein level decreased. We also found that XBP1s was not cleaved directly by 2A{sup pro}, but that cleavage of eukaryotic translation initiation factor 4G by the EV71 2A{sup pro} protein may contribute to the decrease in XBP1s expression. Knockdown of XBP1 increased viral protein expression, and the synthesis of EV71 viral protein and the production of EV71 viral particles were inhibited in XBP1-overexpressing RD cells. When incubated with replication-deficient and UV-irradiated EV71, XBP1-overexpressing RD cells exhibited reduced viral RNA levels, suggesting that the inhibition of XBP1s by viral infection may underlie viral entry, which is required for viral replication. Our findings are the first indication of the ability of XBP1 to inhibit viral entry, possibly via its transcriptional activity in regulating molecules in the endocytic machinery.

  13. Molecular analysis of virulent determinants of enterovirus 71.

    Directory of Open Access Journals (Sweden)

    Renqing Li

    Full Text Available Enterovirus 71 (EV71 is the most important causative agent of hand, foot and mouth disease (HFMD in children. In most cases, it is a self-limiting illness. However some EV71 infectious cases can develop severe clinical outcomes, such as encephalitis, meningitis, poliomyelitis like paralysis, and even death. To identify the determinants of virulence, the deduced amino acid sequence of polyprotein and nucleotide sequence of 5'-NTR and 3'-NTR in 25 SC-EV71 strains (strains from severe cases and 31 MC-EV71 strains (strains from mild cases were analyzed. Results showed four amino acids on two positions (Gly(P710/Gln(P710/Arg(P710 and Glu(P729 on the DE and EF loop of VP1, one (Lys(P930 on the surface of protease 2A and four nucleotides on three positions (G(P272, U(P488 and A(P700/U(P700 in the 5'-NTR region are associated with EV71 virulent phenotype. Predicted secondary structure of RNA using the consensus sequence of 5'-NTR by RNAStructure showed the mutation of nucleotide at position 488 in strain BJ08-Z004-3 (position 491 in prototype strain BrCr can result in the discrepancy of an additional pair of nucleotides and thus change the stability of the second structure of IRES. Fragment base content analysis showed that in the region 696 to 714 bp at the 5'-NTR, where the A(P700/U(P700 was located, the nucleotide constitution ratios differed significantly between SC-EV71 and MC-EV71 strains. In conclusion, comparative genomic analysis showed that virulence of EV71 strains are mainly determined by the amino acids on two positions of VP1, one position of protease 2A and the nucleotides on three positions in 5'-NTR.

  14. Molecular analysis of virulent determinants of enterovirus 71.

    Science.gov (United States)

    Li, Renqing; Zou, Qinghua; Chen, Lijuan; Zhang, Herun; Wang, Yumei

    2011-01-01

    Enterovirus 71 (EV71) is the most important causative agent of hand, foot and mouth disease (HFMD) in children. In most cases, it is a self-limiting illness. However some EV71 infectious cases can develop severe clinical outcomes, such as encephalitis, meningitis, poliomyelitis like paralysis, and even death. To identify the determinants of virulence, the deduced amino acid sequence of polyprotein and nucleotide sequence of 5'-NTR and 3'-NTR in 25 SC-EV71 strains (strains from severe cases) and 31 MC-EV71 strains (strains from mild cases) were analyzed. Results showed four amino acids on two positions (Gly(P710)/Gln(P710)/Arg(P710) and Glu(P729)) on the DE and EF loop of VP1, one (Lys(P930)) on the surface of protease 2A and four nucleotides on three positions (G(P272), U(P488) and A(P700)/U(P700)) in the 5'-NTR region are associated with EV71 virulent phenotype. Predicted secondary structure of RNA using the consensus sequence of 5'-NTR by RNAStructure showed the mutation of nucleotide at position 488 in strain BJ08-Z004-3 (position 491 in prototype strain BrCr) can result in the discrepancy of an additional pair of nucleotides and thus change the stability of the second structure of IRES. Fragment base content analysis showed that in the region 696 to 714 bp at the 5'-NTR, where the A(P700)/U(P700) was located, the nucleotide constitution ratios differed significantly between SC-EV71 and MC-EV71 strains. In conclusion, comparative genomic analysis showed that virulence of EV71 strains are mainly determined by the amino acids on two positions of VP1, one position of protease 2A and the nucleotides on three positions in 5'-NTR.

  15. Aerial gamma ray and magnetic survey: Mississippi and Florida airborne survey, Fort Smith quadrangle, Oklahoma, and Arkansas. Final report

    International Nuclear Information System (INIS)

    The Fort Smith quadrangle in western Arkansas and eastern Oklahoma overlies thick Paleozoic sediments of the Arkoma Basin. These Paleozoics dominate surface exposure except where covered by Quaternary Alluvial materials. Examination of available literature shows no known uranium deposits (or occurrences) within the quadrangle. Seventy-five groups of uranium samples were defined as anomalies and are discussed briefly. None were considered significant, and most appeared to be of cultural origin. Magnetic data show character that suggest structural and/or lithologic complexity, but imply relatively deep-seated sources

  16. Geomorphic Characterization of the Middle Fork Saline River: Garland, Perry, and Saline Counties, Arkansas

    Science.gov (United States)

    Pugh, Aaron L.; Garday, Thomas J.; Redman, Ronald

    2008-01-01

    This report was prepared to help address concerns raised by local residents, State, and Federal agencies about the current geomorphic conditions of the Middle Fork Saline River. Over the past 30 years the Middle Fork Saline River Basin has experienced a marked increase in urbanization. The report summarizes the Middle Fork?s current (2003) channel characteristics at nine stream reaches in the upper 91 square miles of the basin. Assessments at each study reach included comparing measured stream geometry dimensions (cross-sectional area, top width, and mean depth) at bankfull stage to regional hydraulic geometry curves for the Ouachita Mountains Physiographic Province of Arkansas and Oklahoma, evaluations of streambed materials and sinuosity, and classification of individual stream reach types. When compared to the Ouachita Mountains? regional hydraulic geometry curves for natural, stable, stream reaches, five of the nine study reaches had slightly smaller crosssectional areas, longer top widths, and shallower depths. Streambed material analysis indicates that the Middle Fork is a bedrock influenced, gravel dominated stream with lesser amounts of sand and cobbles. Slight increases in sinuosity from 1992 to 2002 at seven of the nine study reaches indicate a slight decrease in stream channel slope. Analyses of the Middle Fork?s hydraulic geometry and sinuosity indicate that the Middle Fork is currently overly wide and shallow, but is slowly adjusting towards a deeper, narrower hydraulic geometry. Using the Rosgen system of channel classification, the two upstream study reaches classified as B4c/1 stream types; which were moderately entrenched, riffle dominated channels, with infrequently spaced pools. The downstream seven study reaches classified as C4/1 stream types; which were slightly entrenched, meandering, gravel-dominated, riffle/ pool channels with well developed flood plains. Analyses of stream reach types suggest that the downstream reaches of the Middle Fork

  17. Morphodynamics of neck cutoffs on elongate meander loops, White River, Arkansas, USA

    Science.gov (United States)

    Konsoer, K. M.

    2015-12-01

    Meander cutoff and oxbow lake formation are essential components of alluvial architecture and riverine habitat of meandering river floodplains. Yet, despite their ubiquitous presence within active floodplains, the detailed processes involved in the initiation of cutoffs and oxbow lakes remain incompletely understood, primarily due to the intermittent nature of such events. Furthermore, conceptual models of meander cutoff and oxbow lake formation have been primarily developed for chute cutoffs and relatively simple planform configurations. Less attention has been given to neck cutoff dynamics occurring on highly sinuous meandering rivers with complex planform morphology. During the formation of a neck cutoff on a compound elongate loop, the upstream and downstream limbs can become oriented roughly subparallel with flow in opposite directions separated by a narrow meander neck. Immediately following cutoff of this thin neck, flow from the upstream limb is sharply redirected into the downstream limb over a short distance. These conditions of tight bend flow should become more pronounced as the ratio of radius of curvature to channel width become smaller, leading to complex patterns of three-dimensional velocities that have implications for the evolution of the cutoff channel and the transformation of the abandoned bend into an oxbow lake. This paper investigates the process dynamics of neck cutoff and oxbow lake formation using detailed field measurements of three-dimensional flow velocities, channel bed topography and geotechnical analysis of the banks and floodplains from three neck cutoffs along the White River, Arkansas (USA), each representing a different stage in the morphologic evolution from cutoff to oxbow lake. Results from this study suggests that the planform geometry of neck cutoff on an elongate meander loop can influence the spatial pattern of sediment deposition within the abandoned loop leading to increased hydrologic connectivity to the main channel

  18. 48 CFR 915.404-4-71-1 - General.

    Science.gov (United States)

    2010-10-01

    ... CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to...

  19. Antiviral Effect of Matrine against Human Enterovirus 71

    Directory of Open Access Journals (Sweden)

    Jiangning Liu

    2012-08-01

    Full Text Available Human enterovirus 71, a member of the Picornaviridae family, is one of the major causative agent of hand, foot and mouth disease in children less than six years old. This illness has caused mortalities in large-scale outbreaks in the Asia-Pacific region in recent years. No vaccine or antiviral therapy is available. In this study, antiviral effect of matrine against enterovirus 71 were evaluated in vitro and in vivo. Matrine could suppress the viral RNA copy number on rhabdomyosarcoma cells. Moreover, matrine treatment of mice challenged with a lethal dose of enterovirus 71 reduced the mortality and relieved clinical symptoms. The results showed that matrine may represent a potential therapeutic agent for enterovirus 71 infection.

  20. Status of research and development of vaccines for enterovirus 71.

    Science.gov (United States)

    Reed, Zarifah; Cardosa, Mary Jane

    2016-06-01

    Although outbreaks of Hand, Foot, and Mouth Disease (HFMD) in young children have long been recognized worldwide, the occurrence of rare and life-threatening neurological, respiratory, and cardiac complications has propelled this common condition into the spotlight as a major public health problem in the affected countries. Various enteroviruses cause HFMD, but the severe complications have been mostly associated with enterovirus 71 (EV71). Medical treatment is supportive and measures to interrupt transmission have been challenging to implement. Preventive vaccines could have an important clinical impact, especially among children younger than 3 years old who are most susceptible to the neurological complications. Several groups in the highly affected Asia-Pacific region are working towards vaccines against EV71 and some candidates have progressed to late-stage clinical trials with two vaccines recently reported to have been approved by the regulatory authorities in China. This report summarizes current issues and progress in the development of vaccines against EV71. PMID:26973065

  1. Enterovirus 71 Brainstem Encephalitis and Cognitive and Motor Deficits

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2006-12-01

    Full Text Available Follow-up studies were conducted in 63 previously healthy children with enterovirus 71 brainstem encephalitis (49 stage II, 7 stage Ilia, and 7 stage Illb at National Cheng Kung University Hospital, Tainan, Taiwan.

  2. 49 CFR 229.71 - Clearance above top of rail.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF TRANSPORTATION RAILROAD LOCOMOTIVE SAFETY STANDARDS Safety Requirements Suspension System § 229.71 Clearance above top of rail. No part or appliance of a locomotive except the wheels,...

  3. Ethanol and water adsorption in methanol-derived ZIF-71

    KAUST Repository

    Lively, Ryan P.

    2011-01-01

    A room temperature method for synthesizing zeolitic imidizolate framework 71 (ZIF-71) is described. The methanol-based synthesis results in >95% yields (based on Zn) and produces crystals with 70% greater surface area than reported earlier. Ethanol uptake into the ZIF compares favorably with a recent modeling-based study. Water uptake was significantly higher than model predictions. © The Royal Society of Chemistry 2011.

  4. Clinical manifestations of CNS infections caused by enterovirus type 71

    OpenAIRE

    Cheol Soon Choi; Yun Jung Choi; Ui Yoon Choi; Ji Whan Han; Dae Chul Jeong; Hyun Hee Kim; Jong Hyun Kim; Jin Han Kang

    2011-01-01

    Purpose: Enterovirus 71, one of the enteroviruses that are responsible for both hand-foot-and-mouth disease and herpangina, can cause neural injury. During periods of endemic spread of hand-foot-andmouth disease caused by enterovirus 71, CNS infections are also frequently diagnosed and may lead to increased complications from neural injury, as well as death. We present the results of our epidemiologic research on the clinical manifestations of children with CNS infections caused by enteroviru...

  5. Atomic Mass and Nuclear Binding Energy for Sr-71 (Strontium)

    Science.gov (United States)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume A `Nuclei with Z = 1 - 54' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms'. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope Sr-71 (Strontium, atomic number Z = 38, mass number A = 71).

  6. Shallow groundwater quality and geochemistry in the Fayetteville Shale gas-production area, north-central Arkansas, 2011

    Science.gov (United States)

    Kresse, Timothy M.; Warner, Nathaniel R.; Hays, Phillip D.; Down, Adrian; Vengosh, Avner; Jackson, Robert B.

    2012-01-01

    The Mississippian Fayetteville Shale serves as an unconventional gas reservoir across north-central Arkansas, ranging in thickness from approximately 50 to 550 feet and varying in depth from approximately 1,500 to 6,500 feet below the ground surface. Primary permeability in the Fayetteville Shale is severely limited, and successful extraction of the gas reservoir is the result of advances in horizontal drilling techniques and hydraulic fracturing to enhance and develop secondary fracture porosity and permeability. Drilling and production of gas wells began in 2004, with a steady increase in production thereafter. As of April 2012, approximately 4,000 producing wells had been completed in the Fayetteville Shale. In Van Buren and Faulkner Counties, 127 domestic water wells were sampled and analyzed for major ions and trace metals, with a subset of the samples analyzed for methane and carbon isotopes to describe general water quality and geochemistry and to investigate the potential effects of gas-production activities on shallow groundwater in the study area. Water-quality analyses from this study were compared to historical (pregas development) shallow groundwater quality collected in the gas-production area. An additional comparison was made using analyses from this study of groundwater quality in similar geologic and topographic areas for well sites less than and greater than 2 miles from active gas-production wells. Chloride concentrations for the 127 groundwater samples collected for this study ranged from approximately 1.0 milligram per liter (mg/L) to 70 mg/L, with a median concentration of 3.7 mg/L, as compared to maximum and median concentrations for the historical data of 378 mg/L and 20 mg/L, respectively. Statistical analysis of the data sets revealed statistically larger chloride concentrations (p-value conservative transport characteristics and relatively elevated concentrations in production waters associated with gas extraction activities. Major ions

  7. Cytokine Immunopathogenesis of Enterovirus 71 Brain Stem Encephalitis

    Directory of Open Access Journals (Sweden)

    Shih-Min Wang

    2012-01-01

    Full Text Available Enterovirus 71 (EV71 is one of the most important causes of herpangina and hand, foot, and mouth disease. It can also cause severe complications of the central nervous system (CNS. Brain stem encephalitis with pulmonary edema is the severe complication that can lead to death. EV71 replicates in leukocytes, endothelial cells, and dendritic cells resulting in the production of immune and inflammatory mediators that shape innate and acquired immune responses and the complications of disease. Cytokines, as a part of innate immunity, favor the development of antiviral and Th1 immune responses. Cytokines and chemokines play an important role in the pathogenesis EV71 brain stem encephalitis. Both the CNS and the systemic inflammatory responses to infection play important, but distinctly different, roles in the pathogenesis of EV71 pulmonary edema. Administration of intravenous immunoglobulin and milrinone, a phosphodiesterase inhibitor, has been shown to modulate inflammation, to reduce sympathetic overactivity, and to improve survival in patients with EV71 autonomic nervous system dysregulation and pulmonary edema.

  8. National Uranium Resource Evaluation Program. Data report: Arkansas, Louisiana, Mississippi, Missouri, Oklahoma, and Texas. Hydrogeochemical and stream sediment reconnaissance

    International Nuclear Information System (INIS)

    This report presents the results of ground water, stream water, and stream sediment reconnaissance in Arkansas, Louisiana, Mississippi, Missouri, Oklahoma, and Texas. The following samples were collected: Arkansas-3292 stream sediments, 5121 ground waters, 1711 stream waters; Louisiana-1017 stream sediments, 0 ground waters, 0 stream waters; Misissippi-0 stream sediments, 814 ground waters, 0 stream waters; Missouri-2162 stream sediments, 3423 ground waters 1340 stream waters; Oklahoma-2493 stream sediments, 2751 ground waters, 375 stream waters; and Texas-279 stream sediments, 0 ground waters, 0 stream waters. Neutron activation analyses are given for U, Br, Cl, F, Mn, Na, Al, V, and Dy in ground water and stream water, and for U, Th, Hf, Ce, Fe, Mn, Na, Sc, Ti, V, Al, Dy, Eu, La, Sm, Yb, and Lu in sediments. The results of mass spectroscopic analysis for He are given for 563 ground water sites in Mississippi. Field measurements and observations are reported for each site. Oak Ridge National Laboratory analyzed sediment samples which were not analyzed by Savannah River Laboratory neutron activation

  9. The Impact Of State Policies On ACA Applications And Enrollment Among Low-Income Adults In Arkansas, Kentucky, And Texas.

    Science.gov (United States)

    Sommers, Benjamin D; Maylone, Bethany; Nguyen, Kevin H; Blendon, Robert J; Epstein, Arnold M

    2015-06-01

    States are taking variable approaches to the Affordable Care Act (ACA) Medicaid expansion, Marketplace design, enrollment outreach, and application assistance. We surveyed nearly 3,000 low-income adults in late 2014 to compare experiences in three states with markedly different policies: Kentucky, which expanded Medicaid, created a successful state Marketplace, and supported outreach efforts; Arkansas, which enacted the private option and a federal-state partnership Marketplace, but with legislative limitations on outreach; and Texas, which did not expand Medicaid and passed restrictions on navigators. We found that application rates, successful enrollment, and positive experiences with the ACA were highest in Kentucky, followed by Arkansas, with Texas performing worst. Limited awareness remains a critical barrier: Fewer than half of adults had heard some or a lot about the coverage expansions. Application assistance from navigators and others was the strongest predictor of enrollment, while Latino applicants were less likely than others to successfully enroll. Twice as many respondents felt that the ACA had helped them as hurt them (although the majority reported no direct impact), and advertising was strongly associated with perceptions of the law. State policy choices appeared to have had major impacts on enrollment experiences among low-income adults and their perceptions of the ACA. PMID:26056207

  10. Penning-trap Q-value determination of the Ga-71(v, e(-))Ge-71 reaction using threshold charge breeding of on-line produced isotopes

    NARCIS (Netherlands)

    Frekers, D.; Simon, M.C.; Andreoiu, C.; Bale, J. C.; Brodeur, M.; Brunner, T.; Chaudhuri, A.; Chowdhury, U.; Lopez-Urrutia, J. R. Crespo; Delheij, P.; Ejiri, H.; Ettenauer, S.; Gallant, A. T.; Gavrin, V.; Grossheim, A.; Harakeh, M. N.; Jang, F.; Kwiatkowski, A. A.; Lassen, J.; Lennarz, A.; Luichtl, M.; Ma, T.; Macdonald, T. D.; Mane, E.; Robertson, D.; Schultz, B. E.; Simon, V. V.; Teigelhoefer, A.; Dilling, J.

    2013-01-01

    We present a first direct Q-value measurement of the Ga-71(v, e(-))Ge-71 reaction using the TITAN mass-measurement facility at ISAC/TRIUMF. The measurements were performed in a Penning trap on neon-like Ga-71(21+) and Ge-71(22+) using isobar separation of the on-line produced mother and daughter nuc

  11. Stream habitat and water-quality information for sites in the Buffalo River Basin and nearby basins of Arkansas, 2001-2002

    Science.gov (United States)

    Petersen, James C.

    2004-01-01

    The Buffalo River lies in north-central Arkansas and is a tributary of the White River. Stream-habitat and water-quality information are presented for 52 sites in the Buffalo River Basin and adjacent areas of the White River Basin. The information was collected during the summers of 2001 and 2002 to supplement fish community sampling during the same time period.

  12. Viral kinetics of Enterovirus 71 in human habdomyosarcoma cells

    Institute of Scientific and Technical Information of China (English)

    Jing Lu; Ya-Qing He; Li-Na Yi; Hong Zan; Hsiang-Fu Kung; Ming-Liang He

    2011-01-01

    AIM: To characterise the viral kinetics of enterovirus 71 (EV71). METHODS: In this study, human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI). After infection, the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD camera at different time points post viral infection (0, 6, 12, 24 h post infection). Cell growth and viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in both EV71 infected and mock infected cells at each time point. EV71 replication kinetics in RD cells was determined by measuring the total intracellular viral RNA with real-time reverse-transcription polymerase chain reaction (qRT-PCR). Also, the intracellular and extracellular virion RNA was isolated and quantified at different time points to analyze the viral package and secretion. The expression of viral protein was determined by analyze the levels of viral structure protein VP1 with Western blotting. RESULTS: EV71 infection induced a significant CPE as early as 6 h post infection (p.i.) in both RD cells infected with high ratio of virus (MOI 10) and low ratio of virus (MOI 1). In EV71 infected cells, the cell growth was inhibited and the number of viable cells was rapidly decreased in the later phase of infection. EV71 virions were uncoated immediately after entry. The intracellular viral RNA began to increase at as early as 3 h p.i. and the exponential increase was found between 3 h to 6 h p.i. in both infected groups. For viral structure protein synthesis, results from western-blot showed that intracellular viral protein VP1 could not be detected until 6 h p.i. in the cells infected at either MOI 1 or MOI 10; and reached the peak at 9 h p.i. in the cells infected with EV71 at both MOI 1 and MOI 10. Simultaneously, the viral package and secretion were also actively processed as the virus underwent rapid replication. The viral package kinetics

  13. Adaptation of enterovirus 71 to adult interferon deficient mice.

    Directory of Open Access Journals (Sweden)

    Elizabeth A Caine

    Full Text Available Non-polio enteroviruses, including enterovirus 71 (EV71, have caused severe and fatal cases of hand, foot and mouth disease (HFMD in the Asia-Pacific region. The development of a vaccine or antiviral against these pathogens has been hampered by the lack of a reliable small animal model. In this study, a mouse adapted EV71 strain was produced by conducting serial passages through A129 (α/β interferon (IFN receptor deficient and AG129 (α/β, γ IFN receptor deficient mice. A B2 sub genotype of EV71 was inoculated intraperitoneally (i.p. into neonatal AG129 mice and brain-harvested virus was subsequently passaged through 12 and 15 day-old A129 mice. When tested in 10 week-old AG129 mice, this adapted strain produced 100% lethality with clinical signs including limb paralysis, eye irritation, loss of balance, and death. This virus caused only 17% mortality in same age A129 mice, confirming that in the absence of a functional IFN response, adult AG129 mice are susceptible to infection by adapted EV71 isolates. Subsequent studies in adult AG129 and young A129 mice with the adapted EV71 virus examined the efficacy of an inactivated EV71 candidate vaccine and determined the role of humoral immunity in protection. Passive transfer of rabbit immune sera raised against the EV71 vaccine provided protection in a dose dependent manner in 15 day-old A129 mice. Intramuscular injections (i.m. in five week-old AG129 mice with the alum adjuvanted vaccine also provided protection against the mouse adapted homologous strain. No clinical signs of disease or mortality were observed in vaccinated animals, which received a prime-and-boost, whereas 71% of control animals were euthanized after exhibiting systemic clinical signs (P<0.05. The development of this animal model will facilitate studies on EV71 pathogenesis, antiviral testing, the evaluation of immunogenicity and efficacy of vaccine candidates, and has the potential to establish correlates of protection

  14. Characterization of the structure, clean-sand percentage, dissolved-solids concentrations, and estimated quantity of groundwater in the Upper Cretaceous Nacatoch Sand and Tokio Formation, Arkansas

    Science.gov (United States)

    Gillip, Jonathan A.

    2014-01-01

    The West Gulf Coastal Plain, Mississippi embayment, and underlying Cretaceous aquifers are rich in water resources; however, large parts of the aquifers are largely unusable because of large concentrations of dissolved solids. Cretaceous aquifers are known to have large concentrations of salinity in some parts of Arkansas. The Nacatoch Sand and the Tokio Formation of Upper Cretaceous age were chosen for investigation because these aquifers produce groundwater to wells near their outcrops and have large salinity concentrations away from their outcrop areas. Previous investigations have indicated that dissolved-solids concentrations of groundwater within the Nacatoch Sand, 2–20 miles downdip from the outcrop, render the groundwater as unusable for purposes requiring freshwater. Groundwater within the Tokio Formation also exhibits large concentrations of dissolved solids downdip. Water-quality data showing elevated dissolved-solids concentrations are limited for these Cretaceous aquifers because other shallower aquifers are used for water supply. Although not suitable for many uses, large, unused amounts of saline groundwater are present in these aquifers. Historical borehole geophysical logs were used to determine the geologic and hydrogeologic properties of these Cretaceous aquifers, as well as the quality of the groundwater within the aquifers. Based on the interpretation of borehole geophysical logs, in Arkansas, the altitude of the top of the Nacatoch Sand ranges from more than 200 to less than -4,000 feet; the structural high occurs in the outcrop area and the structural low occurs in southeastern Arkansas near the Desha Basin structural feature. The thickness of the Nacatoch Sand ranges from 0 to over 550 feet. The minimum thickness occurs where the formation pinches out in the outcrop area, and the maximum thickness occurs in the southwestern corner of Arkansas. Other areas of large thickness include the area of the Desha Basin structural feature in

  15. Geologic map of the St. Joe quadrangle, Searcy and Marion Counties, Arkansas

    Science.gov (United States)

    Hudson, Mark R.; Turner, Kenzie J.

    2009-01-01

    This map summarizes the geology of the St. Joe 7.5-minute quadrangle in the Ozark Plateaus region of northern Arkansas. Geologically, the area lies on the southern flank of the Ozark dome, an uplift that exposes oldest rocks at its center in Missouri. Physiographically, the St. Joe quadrangle lies within the Springfield Plateau, a topographic surface generally held up by Mississippian cherty limestone. The quadrangle also contains isolated mountains (for example, Pilot Mountain) capped by Pennsylvanian rocks that are erosional outliers of the higher Boston Mountains plateau to the south. Tomahawk Creek, a tributary of the Buffalo River, flows through the eastern part of the map area, enhancing bedrock erosion. Exposed bedrock of this region comprises an approximately 1,300-ft-thick sequence of Ordovician, Mississippian, and Pennsylvanian carbonate and clastic sedimentary rocks that have been mildly deformed by a series of faults and folds. The geology of the St. Joe quadrangle was mapped by McKnight (1935) as part of a larger area at 1:125,000 scale. The current map confirms many features of this previous study, but it also identifies new structures and uses a revised stratigraphy. Mapping for this study was conducted by field inspection of numerous sites and was compiled as a 1:24,000-scale geographic information system (GIS) database. Locations and elevations of sites were determined with the aid of a global positioning satellite receiver and a hand-held barometric altimeter that was frequently recalibrated at points of known elevation. Hill-shade-relief and slope maps derived from a U.S. Geological Survey 10-m digital elevation model as well as U.S. Geological Survey orthophotographs from 2000 were used to help trace ledge-forming units between field traverses within the Upper Mississippian and Pennsylvanian part of the stratigraphic sequence. Strikes and dips of beds were typically measured along stream drainages or at well-exposed ledges. Beds dipping less

  16. The Occurrence of Knickpoints in Soluble Strata in the Buffalo River Basin, Arkansas

    Science.gov (United States)

    Thaler, E.; Covington, M. D.; Myre, J. M.; Perne, M.; Holcomb, G.

    2014-12-01

    Prior field and theoretical work has suggested that bedrock channels adjust to lower stream power when encountering highly soluble strata, exhibiting an increase in channel width and/or a decrease in channel slope. However, in apparent contradiction to this expectation, many channels within the Buffalo River Basin, Arkansas, contain knickpoints, in the form of waterfalls and slot canyons, that are developed at the contact between the Mississippian Batesville Sandstone and the underlying Boone Limestone. To improve understanding of bedrock channel response to contrasts in rock solubility, longitudinal surveys were conducted in three channels that cross the Boone Limestone. Additionally, channel widths and a profile were obtained for the main stem of the Buffalo River using aerial photography and a digital elevation model. Schmidt scores for the Boone and Batesville suggest that the two strata have similar compressive strengths, which is a measure of relative resistance to mechanical erosion. Two of the four studied reaches show significant knickpoint development, and in both cases the basin area above the knickpoint is less than 3 km2. One possible explanation is that these knickpoints have been arrested at a critical threshold basin area. However, at least four other such knickpoints are known from the area, and in all cases the knickpoint is highly correlated to the contact rather than a specific basin area, suggesting that the properties of the strata are an important factor. We identify three potential mechanisms that may often act in concert to develop knickpoints at contacts with underlying soluble rocks. (1) If chemical erosion in the soluble reach outpaces uplift, and knickpoint retreat through the overlying layer is sufficiently slow, then a knickpoint will develop. (2) Karstification can divert geomorphic work to the subsurface, resulting in a steep surface channel and possible stalling of upstream knickpoint migration within the soluble strata. (3) The

  17. Identification of specific antigenic epitope at N-terminal segment of enterovirus 71 (EV-71) VP1 protein and characterization of its use in recombinant form for early diagnosis of EV-71 infection

    OpenAIRE

    Zhang, Jianhua; Jiang, Bingfu; Xu, Mingjie; Dai, Xing; Purdy, Michael A.; Meng, Jihong

    2014-01-01

    Human enterovirus 71 (EV-71) is the main etiologic agent of hand, foot and mouth disease (HFMD). We sought to identify EV-71 specific antigens and develop serologic assays for acute-phase EV-71 infection. A series of truncated proteins within the N-terminal 100 amino acids (aa) of EV-71 VP1 was expressed in Escherichia coli. Western blot (WB) analysis showed that positions around 11–21 aa contain EV-71-specific antigenic sites, whereas positions 1–5 and 51–100 contain epitopes shared with hum...

  18. Producing infectious enterovirus type 71 in a rapid strategy

    Directory of Open Access Journals (Sweden)

    Qin E-De

    2010-06-01

    Full Text Available Abstract Background Enterovirus 71 (EV71 is an etiologic agent of hand-foot-and-mouth disease (HFMD, and recent HFMD epidemics worldwide have been associated with a severe form of brainstem encephalitis associated with pulmonary edema and high case-fatality rates. EV71 contains a positive-sense single-stranded genome RNA of approximately 7400 bp in length which encodes a polyprotein with a single open reading frame (ORF, which is flanked by untranslated regions at both the 5' and 3' ends. Results A long distance RT-PCR assay was developed to amplify the full length genome cDNA of EV71 by using specific primes carrying a SP6 promoter. Then the in vitro synthesized RNA transcripts from the RT-PCR amplicons were then transfected into RD cells to produce the rescued virus. The rescued virus was further characterized by RT-PCR and indirect fluorescent-antibody (IFA assay in comparison with the wild type virus. The rescued viruses were infectious on RD cells and neurovirulent when intracerebrally injected into suckling mice. Conclusions Thus, we established a rapid method to produce the infectious full length cDNA of EV71 directly from RNA preparations and specific mutations can be easily engineered into the rescued enterovirus genome by this method.

  19. Similar protective immunity induced by an inactivated enterovirus 71 (EV71) vaccine in neonatal rhesus macaques and children.

    Science.gov (United States)

    Zhang, Ying; Wang, Lichun; Liao, Yun; Liu, Longding; Ma, Kaili; Yang, Erxia; Wang, Jingjing; Che, Yanchun; Jiang, Li; Pu, Jing; Guo, Lei; Feng, Min; Liang, Yan; Cui, Wei; Yang, Huai; Li, Qihan

    2015-11-17

    During the development of enterovirus 71 (EV71) inactivated vaccine for preventing human hand, foot and mouth diseases (HFMD) by EV71 infection, an effective animal model is presumed to be significant and necessary. Our previous study demonstrated that the vesicles in oral regions and limbs potentially associated with viremia, which are the typical manifestations of HFMD, and remarkable pathologic changes were identified in various tissues of neonatal rhesus macaque during EV71 infection. Although an immune response in terms of neutralizing antibody and T cell memory was observed in animals infected by the virus or stimulated by viral antigen, whether such a response could be considered as an indicator to justify the immune response in individuals vaccinated or infected in a pandemic needs to be investigated. Here, a comparative analysis of the neutralizing antibody response and IFN-γ-specific T cell response in vaccinated neonatal rhesus macaques and a human clinical trial with an EV71 inactivated vaccine was performed, and the results showed the identical tendency and increased level of neutralizing antibody and the IFN-γ-specific T cell response stimulated by the EV71 antigen peptide. Importantly, the clinical protective efficacy against virus infection by the elicited immune response in the immunized population compared with the placebo control and the up-modulated gene profile associated with immune activation were similar to those in infected macaques. Further safety verification of this vaccine in neonatal rhesus macaques and children confirmed the potential use of the macaque as a reliable model for the evaluation of an EV71 candidate vaccine.

  20. SR-71黑鸟飞机

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    SR-71“黑鸟”(BlackBird),美国空军高空高速侦察机。飞行高度达到30000米,最大速度达到3.5倍音速,这称之为“双三”。因此SR-71比现有绝大多数战斗机和防空导弹都要飞得高、飞得快,因此出入敌国领空如入无人之境;在以色列上空侦察以色列核设施时,以军F-14战斗机向它发射了AIM-9“响尾蛇”空空导弹,但是导弹飞得却还比SR-71慢。

  1. An analysis of the design and implementation of elementary science methods class instruction in colleges and universities in Arkansas

    Science.gov (United States)

    Lee, Carole K.

    This study aims to understand the design and implementation of elementary methods classes focused in science instruction by teacher educators in the colleges and universities in the state of Arkansas. All 18 institutions with an Early Childhood Education program approved by the Arkansas Department of Education were reviewed with interviews, site visits and data analysis. The research questions are: (1) What goals do teacher educators express with respect to the preparation of preservice elementary teachers for the teaching of science? (2) What components of methods classes for teaching of elementary science examined relate to each of Feiman-Nemser's conceptual orientations in teacher education? (3) What challenges do teacher educators state that they encounter when they plan and teach the methods classes for teaching elementary science? (4) What specific scientific knowledge or skills are emphasized in the methods classes? Enrollment data of the Early Childhood Education program of the 18 institutions were sought. Data collected from the interviews of 16 teacher educators were transcribed according to Feiman-Nemser's five conceptual orientations in teacher education. The findings reveal the complexities and diverse nature of the science-focused method classes as they are affected by the perceptions and academic backgrounds of the teacher educators, the field experiences that preservice teachers have to teach science in the elementary schools, and the science teaching pedagogy and content knowledge provided by the teacher educators. Results show that not all methods classes are focused mainly in science teaching methods and science knowledge; some are an integration of mathematics and science or a blended curriculum with several disciplines. Most institutions do not provide science-related field experiences for preservice teachers. One common theme that emerges in the lesson observations is that all teacher educators use hands-on activities to illustrate the

  2. Geodatabase of the datasets used to represent the six subunits of the Texas Coastal Uplands and Mississippi Embayment aquifer system, Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, Missouri, Tennessee, and Texas

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This geodatabase includes spatial datasets that represent the Texas Coastal Uplands and Mississippi Embayment aquifer system in the States of Alabama, Arkansas,...

  3. Clinical manifestations of CNS infections caused by enterovirus type 71

    Directory of Open Access Journals (Sweden)

    Cheol Soon Choi

    2011-01-01

    Full Text Available Purpose: Enterovirus 71, one of the enteroviruses that are responsible for both hand-foot-and-mouth disease and herpangina, can cause neural injury. During periods of endemic spread of hand-foot-andmouth disease caused by enterovirus 71, CNS infections are also frequently diagnosed and may lead to increased complications from neural injury, as well as death. We present the results of our epidemiologic research on the clinical manifestations of children with CNS infections caused by enterovirus 71. Methods: The study group consisted of 42 patients admitted for CNS infection by enterovirus 71 between April 2009 and October 2009 at the Department of Pediatrics of 5 major hospitals affiliated with the Catholic University of Korea. We retrospectively reviewed initial symptoms and laboratory findings on admission, the specimen from which enterovirus 71 was isolated, fever duration, admission period, treatment and progress, and complications. We compared aseptic meningitis patients with encephalitis patients. Results: Of the 42 patients (23 men, 19 women, hand-foot-and-mouth disease was most prevalent (n=39, followed by herpangina (n=3, upon initial clinical diagnosis. Among the 42 patients, 15 (35.7% were classified as severe, while 27 (64.3% were classified as mild. Factors such as age, fever duration, presence of seizure, and use of intravenous immunoglobulin (IVIG were statistically different between the 2 groups. Conclusion: Our results indicate that patients with severe infection caused by enterovirus 71 tended to be less than 3 years old, presented with at least 3 days of fever as well as seizure activity, and received IVIG treatment.

  4. SR-71 Research Engineer Marta Bohn-Meyer

    Science.gov (United States)

    1992-01-01

    This 1992 photo shows SR-71 flight engineer Marta Bohn-Meyer in front of one of NASA's SR-71 aircraft on the ramp at the Ames-Dryden Flight Research Facility (later, Dryden Flight Research Center), Edwards, California. An aerospace engineer who has been at Dryden since 1979, Bohn-Meyer is the first female crew member ever assigned to fly in the SR-71. Data from the SR-71 program carried out by NASA will be used to aid designers of future supersonic aircraft and propulsion systems. Two SR-71 aircraft have been used by NASA as testbeds for high-speed and high-altitude aeronautical research. The aircraft, an SR-71A and an SR-71B pilot trainer aircraft, have been based here at NASA's Dryden Flight Research Center, Edwards, California. They were transferred to NASA after the U.S. Air Force program was cancelled. As research platforms, the aircraft can cruise at Mach 3 for more than one hour. For thermal experiments, this can produce heat soak temperatures of over 600 degrees Fahrenheit (F). This operating environment makes these aircraft excellent platforms to carry out research and experiments in a variety of areas -- aerodynamics, propulsion, structures, thermal protection materials, high-speed and high-temperature instrumentation, atmospheric studies, and sonic boom characterization. The SR-71 was used in a program to study ways of reducing sonic booms or over pressures that are heard on the ground, much like sharp thunderclaps, when an aircraft exceeds the speed of sound. Data from this Sonic Boom Mitigation Study could eventually lead to aircraft designs that would reduce the 'peak' overpressures of sonic booms and minimize the startling affect they produce on the ground. One of the first major experiments to be flown in the NASA SR-71 program was a laser air data collection system. It used laser light instead of air pressure to produce airspeed and attitude reference data, such as angle of attack and sideslip, which are normally obtained with small tubes and vanes

  5. Antigenic analysis of divergent genotypes human Enterovirus 71 viruses by a panel of neutralizing monoclonal antibodies: current genotyping of EV71 does not reflect their antigenicity.

    Science.gov (United States)

    Chen, Yixin; Li, Chuan; He, Delei; Cheng, Tong; Ge, Shengxiang; Shih, James Wai-Kuo; Zhao, Qinjian; Chen, Pei-Jer; Zhang, Jun; Xia, Ningshao

    2013-01-01

    In recent year, Enterovirus 71 (EV71)-associated hand, foot and mouth disease (HFMD) has become an important public health issue in China. EV71 has been classified into genotypes A, B1-B5 and C1-C5. With such genetic diversity, whether the convalescent or recovery antibody responses can cross-protect infections from other genotypes remains a question. Understanding of the antigenicity of such diverse genetic EV71 isolates is crucial for the EV71 vaccine development. Here, a total of 186 clones anti-EV71 MAbs was generated and characterized with Western blot and cell-based neutralization assay. Forty neutralizing anti-EV71 MAbs were further used to analyze the antigenic properties of 18 recent EV71 isolates representing seven genotypes in neutralization assay. We found that most neutralizing anti-EV71 MAbs are specific to conformational epitopes. We also classified the 40 neutralizing anti-EV71 MAbs into two classes according to their reactivity patterns with 18 EV71 isolates. Class I MAb can neutralize all isolates, suggesting conserved epitopes are present among EV71. Class II MAb includes four subclasses (IIa-IId) and neutralizes only subgroups of EV71 strains. Conversely, 18 EV71 strains were grouped into antigenic types 1 and four antigenic subtypes (2.1-2.4). These results suggest that the current genotyping of EV71 does not reflect their antigenicity which may be important in the selection of EV71 vaccine strains. This panel of neutralizing anti-EV71 MAbs may be useful for the recognition of emerging antigenic variants of EV71 and vaccine development.

  6. EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge

    OpenAIRE

    Wang, Xiaowen; Xiao, Xiangqian; Zhao, Miao; Wei LIU; Pang, Lin; Sun, Xin; Cen, Shan; Burton B Yang; Huang, Yuming; Sheng, Wang; Zeng, Yi

    2016-01-01

    Background Enterovirus 71 (EV71) is the most common causative pathogens of hand, foot and mouth disease (HFMD) associated with severe neurological complications. There is a great need to develop prophylactic vaccine against EV71 infection. Results EV71 virus-like particle (VLP) was produced in yeast expression system by the co-expression of four EV71 structural proteins VP1–VP4. Immunization with the recombinant VLPs elicited potent anti-EV71 antibody responses in adult mice and anti-VLP sera...

  7. Hydrogeochemical and stream-sediment reconnaissance, orientation study, Ouachita Mountain area, Arkansas. National Uranium Resource Evaluation Program

    International Nuclear Information System (INIS)

    A hydrogeochemical ground water orientation study was conducted in the multi-mineralized area of the Ouachita Mountains, Arkansas in order to evaluate the usefulness of ground water as a sampling medium for uranium exploration in similar areas. Ninety-three springs and nine wells were sampled in Clark, Garland, Hot Springs, Howard, Montgomery, Pike, Polk, and Sevier Counties. Manganese, barite, celestite, cinnabar, stibnite, copper, lead, and zinc are present. The following parameters were determined: pH, conductivity, alkalinity, U, Br, Cl, F, He, Mn, Na, V, Al, Dy, NO3, NH3, SO4, and PO4. The minerals appear to significantly affect the chemistry of the ground water. This report is issued in draft form, without detailed technical and copy editing. This was done to make the report available to the public before the end of the National Uranium Resource Evaluation

  8. Metals, Parasites, and Environmental Conditions Affecting Breeding Populations of Spotted Salamanders (Ambystoma maculatum) in Northern Arkansas, USA.

    Science.gov (United States)

    DeMali, Heather M; Trauth, Stanley E; Bouldin, Jennifer L

    2016-06-01

    The spotted salamander (Ambystoma maculatum) is indigenous to northern Arkansas, and several breeding sites are known to exist in the region. Spotted salamanders (n = 17) were collected and examined for parasites and only three females harbored nematodes (Physaloptera spp.). Chronic aquatic bioassays were conducted using water collected from eight breeding ponds during different hydroperiod events. No lethal or sublethal effects were measured in Ceriodaphnia dubia; however, decreased growth and survival were seen in Pimephales promelas. Aqueous, sediment, and salamander hepatic samples were analyzed for As, Cd, Cu, Pb, and Ni. Metal analysis revealed possible increased metal exposure following precipitation, with greatest metal concentrations measured in sediment samples. Hepatic metal concentrations were similar in parasitized and non-parasitized individuals, and greatest Pb concentrations were measured following normal precipitation events. Determining environmental stressors of amphibians, especially during their breeding and subsequent larval life stage, is imperative to improve species conservation. PMID:26886425

  9. Ecological response to hydrological variability and catchment development: Insights from a shallow oxbow lake in Lower Mississippi Valley, Arkansas.

    Science.gov (United States)

    Bhattacharya, Ruchi; Hausmann, Sonja; Hubeny, J Bradford; Gell, Peter; Black, Jessica L

    2016-11-01

    The ecological response of shallow oxbow lakes to variability in hydrology and catchment development in large river floodplain ecosystems (RFE) in Arkansas remains largely unknown. Investigating these responses will advance our understanding of ecological evolution of oxbow lakes in response to the major environmental drivers, which will establish baseline conditions required to develop effective management practices for RFE. In this pilot study, we examined the potential of using a dated surface sediment core from Adams Bayou, a floodplain lake located within the Cache-Lower White River Ramsar site in SE Arkansas. Stratigraphic records of diatoms and sediment geochemistry were used to ascertain variation in Adams Bayou's ecological condition. During 1968-2008, in response to hydrological and anthropogenic changes, Adams Bayou's diatom assemblages progressed from predominantly benthic (Gomphonema parvulum and Meridion circulare) to primarily planktonic assemblage (Aulacoseira granulata and Cyclotella meneghiniana), along with a decrease in magnetic susceptibility (k) and % silt. Statistical analyses reveled that during 1968-2000, higher hydrological connectivity and catchment alterations drove Adams Bayou's ecosystem. After 2000, lower hydrological connectivity and increase in cultivation were the major drivers. The potential impact of increasing air temperature was also noted. The shift in Adams Bayou from a connected, clear, mesotrophic state to a relatively isolated, turbid and nutrient enriched state is consistent with regime shift models and highlights its sensitivity to a combination of environmental stresses prevalent in the catchment. Although fluvial systems pose challenges in establishing clear chronologies, oxbow lake sediments can be a effective paleoecological archives. Our work provides clear evidence for the change in the ecological character of this wetland of international significance and flags the need for a wider assessment of water bodies

  10. Methods for estimating annual exceedance probability discharges for streams in Arkansas, based on data through water year 2013

    Science.gov (United States)

    Wagner, Daniel M.; Krieger, Joshua D.; Veilleux, Andrea G.

    2016-08-04

    In 2013, the U.S. Geological Survey initiated a study to update regional skew, annual exceedance probability discharges, and regional regression equations used to estimate annual exceedance probability discharges for ungaged locations on streams in the study area with the use of recent geospatial data, new analytical methods, and available annual peak-discharge data through the 2013 water year. An analysis of regional skew using Bayesian weighted least-squares/Bayesian generalized-least squares regression was performed for Arkansas, Louisiana, and parts of Missouri and Oklahoma. The newly developed constant regional skew of -0.17 was used in the computation of annual exceedance probability discharges for 281 streamgages used in the regional regression analysis. Based on analysis of covariance, four flood regions were identified for use in the generation of regional regression models. Thirty-nine basin characteristics were considered as potential explanatory variables, and ordinary least-squares regression techniques were used to determine the optimum combinations of basin characteristics for each of the four regions. Basin characteristics in candidate models were evaluated based on multicollinearity with other basin characteristics (variance inflation factor flood region. Average standard errors of prediction of the generalized least-squares models ranged from 32.76 to 59.53 percent, with the largest range in flood region D. Pseudo coefficients of determination of the generalized least-squares models ranged from 90.29 to 97.28 percent, with the largest range also in flood region D. The regional regression equations apply only to locations on streams in Arkansas where annual peak discharges are not substantially affected by regulation, diversion, channelization, backwater, or urbanization. The applicability and accuracy of the regional regression equations depend on the basin characteristics measured for an ungaged location on a stream being within range of those

  11. Ecological response to hydrological variability and catchment development: Insights from a shallow oxbow lake in Lower Mississippi Valley, Arkansas.

    Science.gov (United States)

    Bhattacharya, Ruchi; Hausmann, Sonja; Hubeny, J Bradford; Gell, Peter; Black, Jessica L

    2016-11-01

    The ecological response of shallow oxbow lakes to variability in hydrology and catchment development in large river floodplain ecosystems (RFE) in Arkansas remains largely unknown. Investigating these responses will advance our understanding of ecological evolution of oxbow lakes in response to the major environmental drivers, which will establish baseline conditions required to develop effective management practices for RFE. In this pilot study, we examined the potential of using a dated surface sediment core from Adams Bayou, a floodplain lake located within the Cache-Lower White River Ramsar site in SE Arkansas. Stratigraphic records of diatoms and sediment geochemistry were used to ascertain variation in Adams Bayou's ecological condition. During 1968-2008, in response to hydrological and anthropogenic changes, Adams Bayou's diatom assemblages progressed from predominantly benthic (Gomphonema parvulum and Meridion circulare) to primarily planktonic assemblage (Aulacoseira granulata and Cyclotella meneghiniana), along with a decrease in magnetic susceptibility (k) and % silt. Statistical analyses reveled that during 1968-2000, higher hydrological connectivity and catchment alterations drove Adams Bayou's ecosystem. After 2000, lower hydrological connectivity and increase in cultivation were the major drivers. The potential impact of increasing air temperature was also noted. The shift in Adams Bayou from a connected, clear, mesotrophic state to a relatively isolated, turbid and nutrient enriched state is consistent with regime shift models and highlights its sensitivity to a combination of environmental stresses prevalent in the catchment. Although fluvial systems pose challenges in establishing clear chronologies, oxbow lake sediments can be a effective paleoecological archives. Our work provides clear evidence for the change in the ecological character of this wetland of international significance and flags the need for a wider assessment of water bodies

  12. Analysis and inundation mapping of the April-May 2011 flood at selected locations in northern and eastern Arkansas and southern Missouri

    Science.gov (United States)

    Westerman, Drew A.; Merriman, Katherine R.; De Lanois, Jeanne L.; Berenbrock, Charles

    2013-01-01

    Precipitation that fell from April 19 through May 3, 2011, resulted in widespread flooding across northern and eastern Arkansas and southern Missouri. The first storm produced a total of approximately 16 inches of precipitation over an 8-day period, and the following storms produced as much as 12 inches of precipitation over a 2-day period. Moderate to major flooding occurred quickly along many streams within Arkansas and Missouri (including the Black, Cache, Illinois, St. Francis, and White Rivers) at levels that had not been seen since the historic 1927 floods. The 2011 flood claimed an estimated 21 lives in Arkansas and Missouri, and damage caused by the flooding resulted in a Federal Disaster Declaration for 59 Arkansas counties that received Federal or State assistance. To further the goal of documenting and understanding floods, the U.S. Geological Survey, in cooperation with the Federal Emergency Management Agency, the U.S. Army Corps of Engineers–Little Rock and Memphis Districts, and Arkansas Natural Resources Commission, conducted a study to summarize meteorological and hydrological conditions before the flood; computed flood-peak magnitudes for 39 streamgages; estimated annual exceedance probabilities for 37 of those streamgages; determined the joint probabilities for 11 streamgages paired to the Mississippi River at Helena, Arkansas, which refers to the probability that locations on two paired streams simultaneously experience floods of a magnitude greater than or equal to a given annual exceedance probability; collected high-water marks; constructed flood-peak inundation maps showing maximum flood extent and water depths; and summarized flood damages and effects. For the period of record used in this report, peak-of-record stage occurred at 24 of the 39 streamgages, and peak-of-record streamflow occurred at 13 of the 30 streamgages where streamflow was determined. Annual exceedance probabilities were estimated to be less than 0.5 percent at three

  13. 7 CFR 1724.71 - Borrower contractual obligations.

    Science.gov (United States)

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND PROCEDURES RUS Contract Forms § 1724.71 Borrower contractual obligations. (a) Loan agreement. As a condition of a loan or... obligation is contained in section 5.16 of the loan contract. To comply with the provisions of the...

  14. 48 CFR 1652.215-71 - Investment Income.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Investment Income. 1652.215... 1652.215-71 Investment Income. As prescribed in 1615.470-1, the following clause shall be inserted in all FEHBP contracts based on cost analysis: Investment Income (JAN 1998) (a) The Carrier shall...

  15. 27 CFR 71.116 - Review by Administrator.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Review by Administrator... Review § 71.116 Review by Administrator. The Administrator, on appeal on petition for review, shall... Administrator in any proceeding in which he has engaged in investigation or prosecution, and in such event...

  16. 27 CFR 71.109 - Notice to Administrator.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Notice to Administrator... Decisions Action by the Appropriate Ttb Officer § 71.109 Notice to Administrator. When the appropriate TTB... of the decision on which it is based to the Administrator. Should such order be subsequently...

  17. 48 CFR 3452.232-71 - Incremental funding.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Incremental funding. 3452... 3452.232-71 Incremental funding. As prescribed in 3452.771, insert the following provision in solicitations: Incremental Funding (AUG 1987) (a) Sufficient funds are not presently available to cover...

  18. 48 CFR 915.404-4-71-6 - Fee base.

    Science.gov (United States)

    2010-10-01

    ... CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... fees on special equipment shall be based on the estimated purchase price of the equipment. (d) The fee... estimate of cost to which a percentage factor is applied to determine maximum fee allowances. The fee...

  19. 14 CFR 93.71 - General operating procedures.

    Science.gov (United States)

    2010-01-01

    ... of Niagara Falls, New York § 93.71 General operating procedures. (a) Flight restrictions are in effect below 3,500 feet MSL in the airspace above Niagara Falls, New York, west of a line from latitude... orbit of the Niagara Falls area below 3,500 feet MSL over the Niagara River. (d) The minimum...

  20. 48 CFR 819.202-71 - Additional contracting officer responsibilities.

    Science.gov (United States)

    2010-10-01

    ... OF VETERANS AFFAIRS SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 819.202-71 Additional..., contracting officers must perform the following functions in support of the small business program: (a) Make maximum use of small business source lists. (b) Assure that small business firms are identified...

  1. Sibelius: Scaramouche op. 71, Neeme Järvi / Rainer Wagner

    Index Scriptorium Estoniae

    Wagner, Rainer

    1991-01-01

    Uuest heliplaadist "Sibelius: Scaramouche op. 71 [World Premiere REcording of the Complete Score]. Hochzeitmarsch aus der Musik zum Drama Die Sprache der Vögel [Wedding March "The Language of the Birds"]. Göteborger Sinfoniker, Neeme Järvi. BIS/Disco-Ccenter CD 502 (WD:68'28") DDD

  2. 48 CFR 1852.243-71 - Shared savings.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Shared savings. 1852.243-71... Shared savings. As prescribed in 1843.7102, insert the following clause: Shared Savings (MAR 1997) (a... services to be provided under the contract. (b) Definitions: (1) Cost savings, as contemplated by...

  3. 22 CFR 71.8 - Assistance to American Red Cross.

    Science.gov (United States)

    2010-04-01

    ... American Red Cross. Officers and employees of the Foreign Service may cooperate fully with the American Red Cross within their respective districts and subject to the limitations prescribed in § 102.806 (22 CFR... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Assistance to American Red Cross. 71.8...

  4. 45 CFR 98.71 - Content of reports.

    Science.gov (United States)

    2010-10-01

    ... Program Reporting Requirements § 98.71 Content of reports. (a) At a minimum, a State or territorial Lead Agency's quarterly case-level report to the Secretary, as required in § 98.70, shall include the... State or territorial Lead Agency's annual aggregate report to the Secretary, as required in §...

  5. 48 CFR 215.404-71-5 - Cost efficiency factor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Cost efficiency factor... Contract Pricing 215.404-71-5 Cost efficiency factor. (a) This special factor provides an incentive for... these efforts (Block 29). (b) To determine if using this factor is appropriate, the contracting...

  6. 48 CFR 452.228-71 - Insurance Coverage.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Insurance Coverage. 452... FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 452.228-71 Insurance Coverage. As prescribed in 428.310, insert the following clause: Insurance Coverage (NOV 1996) Pursuant...

  7. 36 CFR 71.9 - Establishment of recreation use fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Establishment of recreation... THE INTERIOR RECREATION FEES § 71.9 Establishment of recreation use fees. (a) Recreation use fees shall be established by all outdoor recreation administering agencies of the Department of the...

  8. 36 CFR 71.2 - Types of Federal recreation fees.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Types of Federal recreation... INTERIOR RECREATION FEES § 71.2 Types of Federal recreation fees. There shall be three types of Federal recreation fees: (a) Entrance fees, charged either on an annual or single-visit basis, for admission to...

  9. 9 CFR 381.71 - Condemnation on ante mortem inspection.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Condemnation on ante mortem inspection... INSPECTION AND CERTIFICATION POULTRY PRODUCTS INSPECTION REGULATIONS Ante Mortem Inspection § 381.71 Condemnation on ante mortem inspection. (a) Birds plainly showing on ante mortem inspection any disease...

  10. 27 CFR 71.69 - Suspension, revocation, or annulment.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Suspension, revocation, or... PROCEEDINGS Hearing Procedure Failure to Appear § 71.69 Suspension, revocation, or annulment. If on the date... Government will proceed ex parte and offer for the record sufficient evidence to make a prima facie case....

  11. 10 CFR 71.13 - Exemption of physicians.

    Science.gov (United States)

    2010-01-01

    ... 10 CFR part 35 or the equivalent Agreement State regulations. ... NUCLEAR REGULATORY COMMISSION (CONTINUED) PACKAGING AND TRANSPORTATION OF RADIOACTIVE MATERIAL Exemptions... medicine is exempt from § 71.5 with respect to transport by the physician of licensed material for use...

  12. 42 CFR 71.52 - Turtles, tortoises, and terrapins.

    Science.gov (United States)

    2010-10-01

    ... (21 CFR 1240.62) regarding general prohibition. (g) Other permits. Permits to import certain species of turtles may be required under other Federal regulations (50 CFR parts 17 and 23) protecting such... 42 Public Health 1 2010-10-01 2010-10-01 false Turtles, tortoises, and terrapins. 71.52 Section...

  13. 28 CFR 71.18 - Authority of the ALJ.

    Science.gov (United States)

    2010-07-01

    ... Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) IMPLEMENTATION OF THE PROVISIONS OF THE PROGRAM FRAUD CIVIL REMEDIES ACT OF 1986 Implementation for Actions Initiated by the Department of Justice § 71... and other procedural matters; (7) Regulate the scope and timing of discovery; (8) Regulate the...

  14. 48 CFR 941.201-71 - Use of subcontracts.

    Science.gov (United States)

    2010-10-01

    ... CATEGORIES OF CONTRACTING ACQUISITION OF UTILITY SERVICES Acquiring Utility Services 941.201-71 Use of... and/or sewerage at facilities owned or leased by DOE shall not be acquired under a subcontract arrangement, except as provided for at 970.4102-1 or if the prime contract is with a utility company....

  15. 19 CFR 151.71 - Laboratory testing for clean yield.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Laboratory testing for clean yield. 151.71 Section... Laboratory testing for clean yield. (a) Test and report by Customs laboratory. The clean yield of all general... clean yield. A report of the percentage clean yield of each general sample as established by the...

  16. 48 CFR 247.301-71 - Evaluation factor or subfactor.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Evaluation factor or... Contracts 247.301-71 Evaluation factor or subfactor. For contracts that will include a significant requirement for transportation of items outside CONUS, include an evaluation factor or subfactor that...

  17. 48 CFR 2152.210-71 - Notice of significant events.

    Science.gov (United States)

    2010-10-01

    ... Standards or other requirements issued by OPM. (b) Upon learning of a significant event, OPM may institute... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Notice of significant... PRECONTRACT PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2152.210-71 Notice of...

  18. Urban Poor Receive RMB7.1 Billion Cash Help

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    All levels of government have teamed up to dole out more than RMB7.1 billion (US$855.42 million) during the ilrst half of this year to help more than 21 million Chinese urban residents who are living below the minimum standard of living.

  19. 7 CFR 1219.71 - Suspension or termination.

    Science.gov (United States)

    2010-01-01

    ... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE HASS AVOCADO PROMOTION, RESEARCH, AND INFORMATION Hass Avocado Promotion, Research, and Information Order Miscellaneous § 1219.71..., have been engaged in the production or importation of Hass avocados. (c) If, as a result of...

  20. 37 CFR 251.71 - Commencement of proceedings.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Royalty Fee Distribution Proceedings § 251.71 Commencement of proceedings. (a) Cable. In the case... year in accordance with the requirements of this subchapter. (b) Satellite carriers. In the case...

  1. Neurologic Manifestations of Enterovirus 71 Infection in Korea.

    Science.gov (United States)

    Lee, Kyung Yeon; Lee, Myoung Sook; Kim, Dong Bin

    2016-04-01

    Enterovirus 71 frequently involves the central nervous system and may present with a variety of neurologic manifestations. Here, we aimed to describe the clinical features, magnetic resonance imaging (MRI) findings, and cerebrospinal fluid (CSF) profiles of patients presenting with neurologic complications of enterovirus 71 infection. We retrospectively reviewed the records of 31 pediatric patients hospitalized with acute neurologic manifestations accompanied by confirmed enterovirus 71 infection at Ulsan University Hospital between 2010 and 2014. The patients' mean age was 2.9 ± 5.5 years (range, 18 days to 12 years), and 80.6% of patients were less than 4 years old. Based on their clinical features, the patients were classified into 4 clinical groups: brainstem encephalitis (n = 21), meningitis (n = 7), encephalitis (n = 2), and acute flaccid paralysis (n = 1). The common neurologic symptoms included myoclonus (58.1%), lethargy (54.8%), irritability (54.8%), vomiting (48.4%), ataxia (38.7%), and tremor (35.5%). Twenty-five patients underwent an MRI scan; of these, 14 (56.0%) revealed the characteristic increased T2 signal intensity in the posterior region of the brainstem and bilateral cerebellar dentate nuclei. Twenty-six of 30 patients (86.7%) showed CSF pleocytosis. Thirty patients (96.8%) recovered completely without any neurologic deficits; one patient (3.2%) died due to pulmonary hemorrhage and shock. In the present study, brainstem encephalitis was the most common neurologic manifestation of enterovirus 71 infection. The characteristic clinical symptoms such as myoclonus, ataxia, and tremor in conjunction with CSF pleocytosis and brainstem lesions on MR images are pathognomonic for diagnosis of neurologic involvement by enterovirus 71 infection. PMID:27051240

  2. The overview of detection methods of Enterovirus 71%肠道病毒71型检测方法研究概况

    Institute of Scientific and Technical Information of China (English)

    吴海波; 郭潮潭

    2008-01-01

    Enterovirus 71(EV71)is an important pathogen of hand-foot-mouth disease(HFMD),several large outbreaks of EV71 infection have caused serious clinical symptoms and sequels,resulted in dozens of deaths.so more and more people pay attention to EV71.The paper reviews about the basic character and detection methods of EV71.%肠道病毒71型(EV71)是引起手足口病(HFMD)的重要病原,已在世界范围内引起了多次大流行,并导致了严重的并发症和后遗症,甚至死亡,引起了人们越来越多的关注.此文就EV71的基本特征和检测方法研究概况作了介绍.

  3. Histopathological features and distribution of EV71 antigens and SCARB2 in human fatal cases and a mouse model of enterovirus 71 infection.

    Science.gov (United States)

    Yu, Pin; Gao, Zifen; Zong, Yuanyuan; Bao, Linlin; Xu, Lili; Deng, Wei; Li, Fengdi; Lv, Qi; Gao, Zhancheng; Xu, Yanfeng; Yao, Yanfeng; Qin, Chuan

    2014-08-30

    Enterovirus 71 (EV71) is a neurotropic pathogen that causes hand, foot, and mouth disease. While infection is usually self-limiting, a minority of patients infected with EV71 develop severe neurological complications. In humans, EV71 has been reported to utilize the scavenger receptor class B, member 2 (SCARB2) as a receptor for infectious cellular entry. In this study, we define the pathological features of EV71-associated disease as well as the distribution of EV71 antigen and SCARB2 in human fatal cases and a mouse model. Histopathologically, human fatal cases showed severe central nervous system (CNS) changes, mainly in the brainstems, spinal cords, and thalamus. These patient further exhibited pulmonary edema and necrotic enteritis. Immunohistochemical analysis of human fatal cases demonstrated that EV71 antigen and SCARB2 were observed mainly in neurons, microglia cells and inflammatory cells in the CNS, and epithelial cells in the intestines. However, skeletal muscle tissue was negative for EV71 antigen. In a mouse model of EV71 infection, we observed massive necrotic myositis, different degrees of viral diseases in CNS, and extensive interstitial pneumonia. In mice, EV71 exhibits strong myotropism compared to the neurotropism seen in humans. EV71 antigen was detected in the spinal cord and brainstem of mice. However, there was no clear correlation between mouse SCARB2 and EV71 antigen distribution in the mouse model, consistent with previous results that SCARB2 functions as a receptor for EV71 in humans but not mice. The EV71-induced lesions seen in the mouse model resembled the pathological changes seen in human samples. These results increase our understanding of EV71 pathogenesis and will inform further work developing a mouse model for EV71 infection.

  4. 41 CFR 301-71.200 - Who must review and sign travel claims?

    Science.gov (United States)

    2010-07-01

    ... travel claims? 301-71.200 Section 301-71.200 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES AGENCY RESPONSIBILITIES 71-AGENCY TRAVEL ACCOUNTABILITY REQUIREMENTS Travel Claims for Reimbursement § 301-71.200 Who must review and sign travel...

  5. New Introductions of Enterovirus 71 Subgenogroup C4 Strains, France, 2012

    OpenAIRE

    Schuffenecker, Isabelle; Henquell, Cécile; Mirand, Audrey; Coste-Burel, Marianne; Marque-Juillet, Stéphanie; Desbois, Delphine; Lagathu, Gisèle; Bornebusch, Laure; Bailly, Jean-Luc; Lina, Bruno

    2014-01-01

    In France during 2012, human enterovirus 71 (EV-A71) subgenogroup C4 strains were detected in 4 children hospitalized for neonatal fever or meningitis. Phylogenetic analysis showed novel and independent EV-A71 introductions, presumably from China, and suggested circulation of C4 strains throughout France. This observation emphasizes the need for monitoring EV-A71 infections in Europe.

  6. Spatio-Temporal Trends of Oak Decline and Mortality under Periodic Regional Drought in the Ozark Highlands of Arkansas and Missouri

    OpenAIRE

    Shifley, Stephen R.; Hong He; W. Keith Moser; Spetich, Martin A.; Michael K. Crosby; Zhaofei Fan; Xiuli Fan

    2012-01-01

    At the forest landscape/region level, based on annual Forest Inventory and Analysis plot data from 1999 to 2010, oak decline and mortality trends for major oak species (groups) were examined in the Ozark Highlands of Arkansas and Missouri. Oak decline has elevated cumulative mortality of red oak species to between 11 and 15 percent in terms of relative density and basal area of standing dead oak trees, respectively. These values are three to five times higher than...

  7. The project for intercomparison of land-surface parameterization schemes (PILPS) phase 2(c) Red-Arkansas River basin experiment: 1. Experiment description and summary intercomparisons

    OpenAIRE

    Wood, EF; Lettenmaier, DP; Liang, X.; D. Lohmann; Boone, A.; Chang, S; Chen, F.; Dai, Y.; Dickinson, RE; Duan, Q; M. Ek; Gusev, YM; Habets, F.; Irannejad, P.; Koster, R

    1998-01-01

    Sixteen land-surface schemes participating in the project for the Intercomparison of Land-surface Schemes (PILPS) Phase 2(c) were run using 10 years (1979-1988) of forcing data for the Red-Arkansas River basins in the Southern Great Plains region of the United States. Forcing data (precipitation, incoming radiation and surface meteorology) and land-surface characteristics (soil and vegetation parameters) were provided to each of the participating schemes. Two groups of runs are presented. (1)...

  8. Recent Progress towards Novel EV71 Anti-Therapeutics and Vaccines

    OpenAIRE

    Qingyong Ng; Fang He; Jimmy Kwang

    2015-01-01

    Enterovirus 71 (EV71) is a group of viruses that belongs to the Picornaviridae family, which also includes viruses such as polioviruses. EV71, together with coxsackieviruses, is widely known for its association with Hand Foot Mouth Disease (HFMD), which generally affects children age five and below. Besides HFMD, EV71 can also trigger more severe and life-threatening neurological conditions such as encephalitis. Considering the lack of a vaccine and antiviral drug against EV71, together with ...

  9. Enterovirus 71-induced autophagy increases viral replication and pathogenesis in a suckling mouse model

    OpenAIRE

    Lee, Ying-Ray; Wang, Po-Shun; Wang, Jen-Ren; Liu, Hsiao-Sheng

    2014-01-01

    Background We previously reported that Enterovirus 71 (EV71) infection activates autophagy, which promotes viral replication both in vitro and in vivo. In the present study we further investigated whether EV71 infection of neuronal SK-N-SH cells induces an autophagic flux. Furthermore, the effects of autophagy on EV71-related pathogenesis and viral load were evaluated after intracranial inoculation of mouse-adapted EV71 (MP4 strain) into 6-day-old ICR suckling mice. Results We demonstrated th...

  10. Status of Water Levels and Selected Water-Quality Conditions in the Sparta-Memphis Aquifer in Arkansas and the Status of Water Levels in the Sparta Aquifer in Louisiana, Spring 2005

    Science.gov (United States)

    Schrader, T.P.; Jones, J.S.

    2007-01-01

    The U.S. Geological Survey in cooperation with the Arkansas Natural Resources Commission, the Arkansas Geological Commission, and the Louisiana Department of Transportation and Development has monitored water levels in the Sparta Sand of Claiborne Group and Memphis Sand of Claiborne Group since the 1920's. Ground-water withdrawals have increased while water levels have declined since monitoring was initiated. This report has been produced to describe ground-water levels in the aquifers in the Sparta Sand and Memphis Sand and provide information for the management of this valuable resource. The 2005 potentiometric-surface map of the aquifers in the Sparta Sand and Memphis Sand was constructed using water-level data collected in 333 wells in Arkansas and 120 wells in Louisiana during the spring of 2005. The highest water-level altitude measured in Arkansas was 327 feet above National Geodetic Vertical Datum of 1929 located in Grant County in the outcrop at the western boundary of the study area; the lowest water-level altitude was 189 feet below National Geodetic Vertical Datum of 1929 in Union County. The highest water-level altitude measured in Louisiana was 246 feet above National Geodetic Vertical Datum of 1929 located in Bossier Parish in the outcrop area near the western boundary of the study area; the lowest water-level altitude was 226 feet below National Geodetic Vertical Datum of 1929 in central Ouachita Parish. Three large depressions centered in Columbia, Jefferson, and Union Counties in Arkansas are the result of large withdrawals for industrial and public supplies. In Louisiana, three major pumping centers are in Ouachita, Jackson, and Lincoln Parishes. Water withdrawals from these major pumping centers primarily is used for industrial and public-supply purposes. Withdrawals from Ouachita and Lincoln Parishes and Union County, Arkansas, primarily for industrial purposes, have caused the resulting cones of depression to coalesce so that the -40 foot

  11. MIXING STUDY FOR JT-71/72 TANKS

    Energy Technology Data Exchange (ETDEWEB)

    Lee, S.

    2013-11-26

    All modeling calculations for the mixing operations of miscible fluids contained in HBLine tanks, JT-71/72, were performed by taking a three-dimensional Computational Fluid Dynamics (CFD) approach. The CFD modeling results were benchmarked against the literature results and the previous SRNL test results to validate the model. Final performance calculations were performed by using the validated model to quantify the mixing time for the HB-Line tanks. The mixing study results for the JT-71/72 tanks show that, for the cases modeled, the mixing time required for blending of the tank contents is no more than 35 minutes, which is well below 2.5 hours of recirculation pump operation. Therefore, the results demonstrate the adequacy of 2.5 hours’ mixing time of the tank contents by one recirculation pump to get well mixed.

  12. Relations between continuous real-time physical properties and discrete water-quality constituents in the Little Arkansas River, south-central Kansas, 1998-2014

    Science.gov (United States)

    Rasmussen, Patrick P.; Eslick, Patrick J.; Ziegler, Andrew C.

    2016-08-11

    Water from the Little Arkansas River is used as source water for artificial recharge of the Equus Beds aquifer, one of the primary water-supply sources for the city of Wichita, Kansas. The U.S. Geological Survey has operated two continuous real-time water-quality monitoring stations since 1995 on the Little Arkansas River in Kansas. Regression models were developed to establish relations between discretely sampled constituent concentrations and continuously measured physical properties to compute concentrations of those constituents of interest. Site-specific regression models were originally published in 2000 for the near Halstead and near Sedgwick U.S. Geological Survey streamgaging stations and the site-specific regression models were then updated in 2003. This report updates those regression models using discrete and continuous data collected during May 1998 through August 2014. In addition to the constituents listed in the 2003 update, new regression models were developed for total organic carbon. The real-time computations of water-quality concentrations and loads are available at http://nrtwq.usgs.gov. The water-quality information in this report is important to the city of Wichita because water-quality information allows for real-time quantification and characterization of chemicals of concern (including chloride), in addition to nutrients, sediment, bacteria, and atrazine transported in the Little Arkansas River. The water-quality information in this report aids in the decision making for water treatment before artificial recharge.

  13. Simulated response to pumping stress in the Sparta aquifer of southeastern Arkansas and north-central Louisiana, 1998-2027

    Science.gov (United States)

    Hays, Phillip D.; Lovelace, John K.; Reed, Thomas B.

    1998-01-01

    The Sparta aquifer in southeastern Arkansas and north-central Louisiana is a major water resource for municipal, industrial, and agricultural uses. In recent years, the demand for water in some areas has resulted in withdrawals from the Sparta that significantly exceed recharge to the aquifer. Considerable drawdown has occurred in the potentiometric surface, and water users and managers alike have begun to question the ability of the aquifer to supply water for the long term. Large cones of depression are centered beneath the Grand Prairie area and the cities of Pine Bluff and El Dorado in Arkansas, and Monroe in Louisiana. Water levels in the aquifer have declined at rates greater than 1 foot per year for more than a decade in much of southern Arkansas and northern Louisiana and are now below the top of the formation in parts of Union and Columbia Counties, Arkansas, and in several areas of Louisiana. Problems related to over draft in the Sparta could result in increased drilling and pumping costs, loss of yield, salt-water intrusion, and decrease in water quality in areas of large drawdown. The effects of current ground-water withdrawals and potential future withdrawals on water availability are major concerns of water managers and users as well as the general public in the two States. The Sparta model-a regional scale, digital ground-water flow model-was first calibrated in the mid-1980's. The model was updated and reverified using 1995-97 data. Visual inspection of the observed (1996-97) and simulated potentiometric surfaces, statistical analysis of the error for the original calibration and current reverification, and comparison of observed versus simulated hydro graphs indicates that the model is simulating conditions in the aquifer within acceptable error, and the quality of current (1998) model results is similar to the original model calibration results. When stressed with current withdrawal volumes and distributions, the model is able to simulate

  14. The approved pediatric drug suramin identified as a clinical candidate for the treatment of EV71 infection-suramin inhibits EV71 infection in vitro and in vivo.

    Science.gov (United States)

    Ren, Peijun; Zou, Gang; Bailly, Benjamin; Xu, Shanshan; Zeng, Mei; Chen, Xinsheng; Shen, Liang; Zhang, Ying; Guillon, Patrice; Arenzana-Seisdedos, Fernando; Buchy, Philippe; Li, Jian; von Itzstein, Mark; Li, Qihan; Altmeyer, Ralf

    2014-09-01

    Enterovirus 71 (EV71) causes severe central nervous system infections, leading to cardiopulmonary complications and death in young children. There is an urgent unmet medical need for new pharmaceutical agents to control EV71 infections. Using a multidisciplinary approach, we found that the approved pediatric antiparasitic drug suramin blocked EV71 infectivity by a novel mechanism of action that involves binding of the naphtalentrisulonic acid group of suramin to the viral capsid. Moreover, we demonstrate that when suramin is used in vivo at doses equivalent to or lower than the highest dose already used in humans, it significantly decreased mortality in mice challenged with a lethal dose of EV71 and peak viral load in adult rhesus monkeys. Thus, suramin inhibits EV71 infection by neutralizing virus particles prior to cell attachment. Consequently, these findings identify suramin as a clinical candidate for further development as a therapeutic or prophylactic treatment for severe EV71 infection.

  15. Sediment Quality and Comparison to Historical Water Quality, Little Arkansas River Basin, South-Central Kansas, 2007

    Science.gov (United States)

    Juracek, Kyle E.; Rasmussen, Patrick P.

    2008-01-01

    The spatial and temporal variability in streambed-sediment quality and its relation to historical water quality was assessed to provide guidance for the development of total maximum daily loads and the implementation of best-management practices in the Little Arkansas River Basin, south-central Kansas. Streambed-sediment samples were collected at 26 sites in 2007, sieved to isolate the less than 63-micron fraction (that is, the silt and clay), and analyzed for selected nutrients (total nitrogen and total phosphorus), organic and total carbon, 25 trace elements, and the radionuclides beryllium-7, cesium-137, lead-210, and radium-226. At eight sites, streambed-sediment samples also were collected and analyzed for bacteria. Particulate nitrogen, phosphorus, and organic carbon concentrations in the streambed sediment varied substantially spatially and temporally, and positive correlations among the three constituents were statistically significant. Along the main-stem Little Arkansas River, streambed-sediment concentrations of particulate nitrogen and phosphorus generally were larger at and downstream from Alta Mills, Kansas. The largest particulate nitrogen concentrations were measured in samples collected in the Emma Creek subbasin and may be related to livestock and poultry production. The largest particulate phosphorus concentrations in the basin were measured in samples collected along the main-stem Little Arkansas River downstream from Alta Mills, Kansas. Particulate nitrogen, phosphorus, and organic carbon content in the water and streambed-sediment samples typically decreased as streamflow increased. This inverse relation may be caused by an increased contribution of sediment from channel-bank sources during high flows and (or) increased particle sizes transported by the high flows. Trace element concentrations in the streambed sediment varied from site to site and typically were less than threshold-effects guidelines for possible adverse biological effects

  16. [Establishment of EV71 animal models with 2-week-old BALB/c mice].

    Science.gov (United States)

    Wang, Hui-Qiang; Jiang, Jian-Dong; Li, Yu-Huan

    2013-03-01

    Animal model is very important for anti-EV71 (enterovirus 71) drug and vaccine development. 1-day-old suckling EV71 mouse model is the main in vivo model used in China. 1-day-old suckling EV71 mouse is too small to perform antiviral experiment. And the route of administration and dosage capacity are also restricted. A strong virulence EV71 virus strain was selected after screening from five EV71 strains with 1-day-old suckling mice. A mouse-adapted EV71 strain with increased virulence in 12-day-old suckling mice, EV71-M5, was generated after five serial passages of the parental EV71 strain in mice. Virus titers of EV71 infected mice heart, liver, spleen, lung, kidney, small intestine, brain and muscle tissue were determined by cytopathic effect (CPE) assay. The virus used in this model is the first isolated EV71 strain in China. And 2-week-old suckling mice were used in this model. This is a supplement for the EV71 animal model in China. Establishment of this EV71 model will provide an attractive platform for anti-EV71 vaccine and drug development.

  17. Comparing the 2000 and 2005 factors affecting the selling price of feeder cattle sold at Arkansas livestock auctions.

    Science.gov (United States)

    Troxel, T R; Barham, B L

    2007-12-01

    The objectives of the study were to determine how factors affecting the selling price of feeder calves changed from 2000 to 2005 and to examine the perception that discounts narrow or even disappear as calf supplies decrease and selling prices increase. Data from weekly Arkansas livestock auctions were collected from January 1 to December 31 in 2000 and 2005. Data included calf sex, breed type, color, muscle score, horn status, frame score, fill, condition, health, and BW. Mean selling prices for 2000 and 2005 were $92.91 +/- 15.05 and $118.32 +/- 15.13 (mean +/- SD; $/45.45 kg), respectively. Individual price observations were subtracted from the respective annual means and became the dependent variable. The selling prices for feeder calves sold in groups of 2 to 5 calves and in groups of >/= 6 calves were greater in 2005 than 2000 (P Brahman (P Brahman Cross, Charolais, Charolais x Limousin, Hereford x Limousin, Limousin, Limousin x one-fourth Brahman, Longhorn, Saler and Simmental. Yellow-white face, black-white face, black, and gray feeder calves received an increase in selling price from 2000 to 2005 (P < 0.001). Although fewer horned feeder calves were sold in 2005 (P < 0.01), they received greater discounts in 2005 than 2000 (-$2.86 +/- 0.16 and -$0.51 +/- 0.09; P < 0.001). In 2005, large-framed feeder calves did not receive the premium detected in 2000, but medium-framed feeder calves in 2005 received a greater selling price compared with 2000. Feeder calves with a muscle score of 1 received a greater premium in 2005 compared with 2000 ($2.58 +/- 0.06 and $0.02 +/- 0.09, respectively; P < 0.001). Feeder calves with a muscle score of 2 were discounted in both years, but the discount in 2005 was not as great as in 2000 (P < 0.001). Full and tanked feeder calves received greater discounts in 2005 than in 2000 (P < 0.001). Discounts for fleshy and fat feeder calves were greater in 2005 than in 2000. Most factors affecting the selling price of Arkansas feeder

  18. Comparing the 2000 and 2005 factors affecting the selling price of feeder cattle sold at Arkansas livestock auctions.

    Science.gov (United States)

    Troxel, T R; Barham, B L

    2007-12-01

    The objectives of the study were to determine how factors affecting the selling price of feeder calves changed from 2000 to 2005 and to examine the perception that discounts narrow or even disappear as calf supplies decrease and selling prices increase. Data from weekly Arkansas livestock auctions were collected from January 1 to December 31 in 2000 and 2005. Data included calf sex, breed type, color, muscle score, horn status, frame score, fill, condition, health, and BW. Mean selling prices for 2000 and 2005 were $92.91 +/- 15.05 and $118.32 +/- 15.13 (mean +/- SD; $/45.45 kg), respectively. Individual price observations were subtracted from the respective annual means and became the dependent variable. The selling prices for feeder calves sold in groups of 2 to 5 calves and in groups of >/= 6 calves were greater in 2005 than 2000 (P x Hereford, Angus, Angus x Charolais, and Brahman (P Brahman Cross, Charolais, Charolais x Limousin, Hereford x Limousin, Limousin, Limousin x one-fourth Brahman, Longhorn, Saler and Simmental. Yellow-white face, black-white face, black, and gray feeder calves received an increase in selling price from 2000 to 2005 (P < 0.001). Although fewer horned feeder calves were sold in 2005 (P < 0.01), they received greater discounts in 2005 than 2000 (-$2.86 +/- 0.16 and -$0.51 +/- 0.09; P < 0.001). In 2005, large-framed feeder calves did not receive the premium detected in 2000, but medium-framed feeder calves in 2005 received a greater selling price compared with 2000. Feeder calves with a muscle score of 1 received a greater premium in 2005 compared with 2000 ($2.58 +/- 0.06 and $0.02 +/- 0.09, respectively; P < 0.001). Feeder calves with a muscle score of 2 were discounted in both years, but the discount in 2005 was not as great as in 2000 (P < 0.001). Full and tanked feeder calves received greater discounts in 2005 than in 2000 (P < 0.001). Discounts for fleshy and fat feeder calves were greater in 2005 than in 2000. Most factors

  19. Potential for Optical Sensor-Based Nitrogen Fertilization in Grain Sorghum (Sorghum bicolor L. Moench) in Arkansas

    Science.gov (United States)

    Rosales Rodriguez, Kamil

    Ground-based active-optical (GBAO) crop sensors have become an effective tool to improve nitrogen (N) use efficiency and to predict yield early in the growing season, particularly for grass crops. Commercially available canopy sensors calculate the normalized difference vegetative index (NDVI) by emitting light in the red and near infrared range of the electromagnetic spectrum. The NDVI is used to evaluate vigor status and to estimate yield potential. However, few studies have been conducted to compare the performance of commercially available sensors. Therefore, a study was conducted using the most common crop canopy sensors: i) N-Tech's GreenSeeker(TM) (GS), ii) Holland Scientific's Crop Circle(TM) (CC), and iii) Minolta's SPAD-502 chlorophyll content meter (CCM). The objective of this study was to find the optimum time for sensing and compare the relative performance of the sensors in estimating the yield potential of grain sorghum (Sorghum bicolor L. Moench). Treatments included six levels of N fertilization (0, 37, 74, 111, 148, and 185 kg N/ ha), applied in a single split 20 days after planting (DAP). Treatments were arranged in a randomized complete block design with five replications, in four locations in Arkansas, during 2012 and 2013. Sensors readings at vegetative growth stages V3, 4, 5 and 6. Results from simple regression analysis showed that the V3-V4 growth stage correlated better with grain yield than readings collected and any other time. In season estimated yield (INSEY) obtained at V3 captured 41, 57, 78, and 61% of the variation in grain sorghum yield when red NDVI of GS, red NDVI of CC, red edge for CC and CCM, respectively, were used. Results from these studies suggest that the CC sensor has a better potential for in-season site-specific N application in Arkansas than the GS sensor. The GS reflectance values appear to saturate after the V3 stage, in contrast with CC values that allow for discrimination past the V3 Stage. Therefore, the red

  20. Methods for estimating annual exceedance probability discharges for streams in Arkansas, based on data through water year 2013

    Science.gov (United States)

    Wagner, Daniel M.; Krieger, Joshua D.; Veilleux, Andrea G.

    2016-08-04

    In 2013, the U.S. Geological Survey initiated a study to update regional skew, annual exceedance probability discharges, and regional regression equations used to estimate annual exceedance probability discharges for ungaged locations on streams in the study area with the use of recent geospatial data, new analytical methods, and available annual peak-discharge data through the 2013 water year. An analysis of regional skew using Bayesian weighted least-squares/Bayesian generalized-least squares regression was performed for Arkansas, Louisiana, and parts of Missouri and Oklahoma. The newly developed constant regional skew of -0.17 was used in the computation of annual exceedance probability discharges for 281 streamgages used in the regional regression analysis. Based on analysis of covariance, four flood regions were identified for use in the generation of regional regression models. Thirty-nine basin characteristics were considered as potential explanatory variables, and ordinary least-squares regression techniques were used to determine the optimum combinations of basin characteristics for each of the four regions. Basin characteristics in candidate models were evaluated based on multicollinearity with other basin characteristics (variance inflation factor < 2.5) and statistical significance at the 95-percent confidence level (p ≤ 0.05). Generalized least-squares regression was used to develop the final regression models for each flood region. Average standard errors of prediction of the generalized least-squares models ranged from 32.76 to 59.53 percent, with the largest range in flood region D. Pseudo coefficients of determination of the generalized least-squares models ranged from 90.29 to 97.28 percent, with the largest range also in flood region D. The regional regression equations apply only to locations on streams in Arkansas where annual peak discharges are not substantially affected by regulation, diversion, channelization, backwater, or urbanization

  1. Preparation of the EV71 VLPs%新肠道病毒71型病毒样颗粒的制备

    Institute of Scientific and Technical Information of China (English)

    李晓楠; 曹明慧; 李培锋

    2012-01-01

    目的:研究新肠道病毒71型(Enterovirus 71,EV71)病毒样颗粒(Virus-like particles,VLPs)的制备方法.方法:依据昆虫细胞偏爱密码子优化EV71 P1和EV71 3CD基因,将二者分别克隆至供体质粒pFastBac Dual多角体蛋白启动子pPh和pP10上;构建重组pFastBac Dual EV71 P1-3CD质粒,并与Bacmid质粒转座后,获得重组杆状病毒表达质粒转染至Sf9细胞,应用PCR、免疫荧光、SDS-Page和Western blot方法鉴定EV71 VLPs表达情况,最后利用蔗糖密度梯度离心的方法纯化EV71 VLPs.结果:经昆虫密码子优化的EV71 P1和EV71 3CD基因,利用Bac-to-Bac系统成功组装了EV71 VLPs,纯化EV71 VLPs的浓度为0.817 mg/ml,纯度>90%.结论:成功地构建了重组杆状病毒EV71 P1-3CD Bacmid表达质粒,并表达和纯化了 EV71 VLPs,这为今后EV71 VLPs疫苗的研究奠定了基础.%Objective: The aim of the present study was to prepared EV71 VLPs, which was important for further studies in term of EV71 VLPs. Methods: Based on preferred codon optimization of insect cells ,EV71 P1 and EV71 3 CD genes were cloned into the donor vector pFastBac Dual under the polyhedrin promoter pPh and pP 10, and the constructed donor plasmid pFastBac Dual -P1 -3CD was transformed to E. Coli DHlOBac. The obtained recombinant baculovirus plasmid Bacmid -P1-3CD was transfected into Sf9 cells to prepare recombinant baeulovirus and the expressed products were identified by PCR , IFA, SDS-Page and Western -Blot. Then the VLPs were purified by sucrose density gradient centrifugation . Results:The EV71 P1 and EV71 3CD genes insects codon optimized were assembled EV71 VLPs successfully by Bac-to-Bac system. Concentration of purified EV71 VLPs was 0. 817 mg/ml, purity of 90%. Conclusion:Finally, the pFastBac Dual EV71 P1-3CD was constructed , and EV71 VLPs were expressed and purified successfully, which provide a valuable platform for the future EV71 VLPs vaccine research.

  2. RASSF4 promotes EV71 replication to accelerate the inhibition of the phosphorylation of AKT.

    Science.gov (United States)

    Zhang, Fengfeng; Liu, Yongjuan; Chen, Xiong; Dong, Lanlan; Zhou, Bingfei; Cheng, Qingqing; Han, Song; Liu, Zhongchun; Peng, Biwen; He, Xiaohua; Liu, Wanhong

    2015-03-20

    Enterovirus 71 (EV71) is a neurotropic virus that causes hand, foot and mouth disease (HFMD), occasionally leading to death. As a member of the RAS association domain family (RASSFs), RASSF4 plays important roles in cell death, tumor development and signal transduction. However, little is known about the relationship between RASSF4 and EV71. Our study reveals for the first time that RASSF4 promotes EV71 replication and then accelerates AKT phosphorylation inhibition in EV71-infected 293T cells, suggesting that RASSF4 may be a potential new target for designing therapeutic measures to prevent and control EV71 infection.

  3. 抗肠道病毒71型药物研究进展%Research Progress on Anti-EV71 Agents

    Institute of Scientific and Technical Information of China (English)

    何静; 周志统; 袁正宏

    2011-01-01

    肠道病毒71型(EV71)是引起手足口病的主要病原体,并且EV71引起的神经系统并发症所造成的危害也越来越引起人们的重视.但是迄今尚无有效的EV71疫苗和特效抗EV71药物.目前,国内外针对EV71复制周期不同靶点的抗病毒药物研究取得了一定的突破.现将受体阻断剂、病毒衣壳结合剂、蛋白酶抑制剂、3DRNA多聚酶抑制剂、利巴韦林、RNA干扰技术以及中药等抗EV71的研究进展进行综述.%EV71 is the main etiological agent of hand , foot and mouth disease( HFMD ) , people pay more and more attention on the harm of EV71 caused neurological complications. But there are still lacking of effective EV71 vaccine and anti-EV71 drug. Currently,domestic and overseas research group have achieved some breakthrough progress on anti-EV71 drug research which target different site of viral replication cycle.This article reviewed recent studies of research progress on anti-EV71 agents which based on various molecular targets. Including receptor inhibitors , vial capsid-binding molecules , 3C protease inhibitor , 3 D RNA polymerase inhibitor , ribavirin, RNA interference and traditional Chinese medcine.

  4. Callisto - Disk temperature at 3.71-centimeter wavelength

    Science.gov (United States)

    Berge, G. L.; Muhleman, D. O.

    1975-01-01

    We observed the radio emission of Callisto with a three-element interferometer at the time of the 1973 opposition of Jupiter. Special care was taken to remove the residual, unresolved contribution from Jupiter itself in the antenna side lobes. The resulting disk temperature at a wavelength of 3.71 centimeters, assuming a radius of 2500 + or - 75 kilometers for Callisto, was 101 + or - 25 K. This temperature is much more consistent with emission from a simple dielectric sphere than the considerably higher temperatures that have been reported for wavelengths of 3.5 and 8.2 millimeters.

  5. Inter-state return migration in India: 1961-71.

    Science.gov (United States)

    Sivamurthy, M; Kadi, A S

    1984-12-01

    "In this paper an attempt has been made to estimate return migration from the destination state of migrants for 15 major Indian states during 1961-71 among the life-time inter-state migrants enumerated in the 1961 census. The model used for estimation is based on the procedure suggested by Zachariah (1967) for estimating return migration from two census data." The results indicate that of the almost 13 million interstate migrants enumerated in the 1961 census, about 4.7 million returned during the period 1961-1971. Factors affecting this return migration are considered.

  6. 'n Eksegese van Jeremia 7:1–15

    Directory of Open Access Journals (Sweden)

    A. A. da Silva

    1985-01-01

    Full Text Available Jeremia 7:1–1 5 is a judgment prophecy in the style of a court verdict. Each of the five subdivisions is functional: The introduction underlines the divine authority of the judgment; the part between the introduction and the charge states the 'Law' by which the nation's transgressions would be tried; the charge stipulates the nature and gravity of the transgressions; the part between the charge and the sentence quotes a previous 'case' and the sentence stipulates that the punishment will be the same as the punishment executed against Shilo: the temple will be destroyed, and the inhabitants of Judah will be rejected.

  7. EPSPS Gene Amplification in Glyphosate-Resistant Italian Ryegrass (Lolium perenne ssp. multiflorum) Populations from Arkansas (United States).

    Science.gov (United States)

    Salas, Reiofeli A; Scott, Robert C; Dayan, Franck E; Burgos, Nilda R

    2015-07-01

    Glyphosate-resistant Italian ryegrass was detected in Arkansas (United States) in 2007. In 2014, 45 populations were confirmed resistant in eight counties across the state. The level of resistance and resistance mechanisms in six populations were studied to assess the severity of the problem and identify alternative management approaches. Dose-response bioassays, glyphosate absorption and translocation experiments, herbicide target (EPSPS) gene sequence analysis, and gene amplification assays were conducted. The dose causing 50% growth reduction (GR50) was 7-19 times higher for the resistant population than for the susceptible standard. Uptake and translocation of (14)C-glyphosate were similar in resistant and susceptible plants, and no mutation in the EPSPS gene known to be associated with resistance to glyphosate was detected. Resistant plants contained from 11- to >100-fold more copies of the EPSPS gene than the susceptible plants, whereas the susceptible plants had only one copy of EPSPS. Plants surviving the recommended dose of glyphosate contained at least 10 copies. The EPSPS copy number was positively related to glyphosate resistance level (r = 80). Therefore, resistance to glyphosate in these populations is due to multiplication of the target site. Resistance mechanisms could be location-specific. Suppressing the mechanism for gene amplification may overcome resistance.

  8. Perceived Need for Substance Abuse Treatment among Illicit Stimulant Drug Users in Rural Areas of Ohio, Arkansas, and Kentucky

    Science.gov (United States)

    Falck, Russel S.; Wang, Jichuan; Carlson, Robert G.; Krishnan, Laura L.; Leukefeld, Carl; Booth, Brenda M.

    2007-01-01

    Non-medical drug use in rural communities in the United States is a significant and growing public health threat. Understanding what motivates drug users in rural areas to seek substance abuse treatment may help in addressing the problem. Perceived need for treatment, a construct indicative of problem recognition and belief in problem solution, has been identified as an important predictor of help-seeking behavior. This cross-sectional study used data collected through face-to-face interviews to examine factors associated with perceived need for drug abuse treatment among not-in-treatment, adult, illicit stimulant drug users (n=710) in rural areas of Ohio, Kentucky, and Arkansas. More than one-quarter of the sample perceived a need for treatment. Results from a stepwise multiple regression analysis showed that white users, users with better physical and mental health status, and occasional users of methamphetamine were significantly less likely to see a need for treatment. Users with higher Addiction Severity Index composite scores for family/social problems or legal problems, and users with prior drug abuse treatment experience were significantly more likely to perceive a need for treatment. These findings have practical implications for efforts addressing substance abuse in rural areas. PMID:17604917

  9. Disposal of chemical agents and munitions stored at Pine Bluff Arsenal, Pine Bluff, Arkansas. Final phase 1, Environmental report

    Energy Technology Data Exchange (ETDEWEB)

    Ensminger, J.T.; Hillsman, E.L.; Johnson, R.D.; Morrisey, J.A.; Staub, W.P.; Boston, C.R.; Hunsaker, D.B.; Leibsch, E.; Rickert, L.W.; Tolbert, V.R.; Zimmerman, G.P.

    1991-09-01

    The Pine Bluff Arsenal (PBA) near Pine Bluff, Arkansas, is one of eight continental United States (CONUS) Army installations where lethal unitary chemical agents and munitions are stored and where destruction of agents and munitions is proposed under the Chemical Stockpile Disposal Program (CSDP). The chemical agent inventory at PBA consists of approximately 12%, by weight, of the total US stockpile. The destruction of the stockpile is necessary to eliminate the risk to the public from continued storage and to dispose of obsolete and leaking munitions. In 1988 the US Army issued a Final Programmatic Environmental Impact Statement (FPEIS) for the CSDP that identified on-site disposal of agents and munitions as the environmentally preferred alternative (i.e., the alternative with the least potential to cause significant adverse impacts). The purpose of this report is to examine the proposed implementation of on-site disposal at PBA in light of more recent and more detailed data than those on which the FPEIS is based. New population data were used to compute fatalities using the same computation methods and values for all other parameters as in the FPEIS. Results indicate that all alternatives are indistinguishable when the potential health impacts to the PBA community are considered. However, risks from on-site disposal are in all cases equal to or less than risks from other alternatives. Furthermore, no unique resources with the potential to prevent or delay implementation of on-site disposal at PBA have been identified.

  10. Hydrogeology and physical characteristics of water samples at the Red River aluminum site, Stamps, Arkansas, April 2001

    Science.gov (United States)

    Czarnecki, John B.; Stanton, Gregory P.; Freiwald, David A.

    2001-01-01

    The Red River Aluminum site near Stamps, Arkansas, contains waste piles of salt cake and metal byproducts from the smelting of aluminum. The waste piles are subjected to about 50 inches of rainfall a year, resulting in the dissolution of the salts and metal. To assess the potential threat to underlying ground-water resources at the site, its hydrogeology was characterized by measuring water levels and field parameters of water quality in 23 wells and at 2 surface-water sites. Seventeen of these monitor wells were constructed at various depths for this study to allow for the separate characterization of the shallow and deep ground-water systems, the calculation of vertical gradients, and the collection of water samples at different depths within the flow system. Lithologic descriptions from drill-hole cuttings and geophysical logs indicate the presence of interbedded sands, gravels, silts, and clays to depths of 65 feet. The regionally important Sparta aquifer underlies the site. Water levels in shallow wells indicate radial flow away from the salt-cake pile located near the center of the site. Flow in the deep system is to the west and southwest toward Bodcau Creek. Water-level data from eight piezometer nests indicate a downward hydraulic gradient from the shallow to deep systems across the site. Values of specific conductance (an indicator of dissolved salts) ranged from 215 to 196,200 microsiemens per centimeter and indicate that saline waters are being transported horizontally and vertically downward away from the site

  11. Arkansas Nuclear One IPE: An implementation of the EPRI generic framework for IPE back end (Level 2) analysis

    International Nuclear Information System (INIS)

    In Generic Letter 88-20, the NRC asked US nuclear power plant licensees to prepare individual plant probabilistic risk studies. These studies are commonly referred to as Individual Plant Examinations (IPEs). In response to the IPE Generic Letter, Energy Operations implemented an EPRI-developed generic framework for the Level 2 analysis portion of the Arkansas Nuclear One (ANO Units 1 and 2) IPEs. It begins with grouping the Level 1 core damage accident sequences having similar characteristics into a more manageable set of plant damage states (PDSs). This serves as the interface with the Level 1 PRA, and it provides the starting point for investigating the various severe accident progression scenarios and containment response. These investigations are aided by the use of the generic containment event trees (CETs). This paper presents the implementation of the generic framework for the ANO-2 IPE and describes the integrated Level 1/2 insights on the risk-dominant sequences, potential containment vulnerabilities, and identification of likely accident management strategies. The present understanding of some severe accident phenomena is still limited. Therefore, a parametric analysis of the ANO IPE Level 2 model, as intended in the generic framework, was conducted reflecting different viewpoints in the severe accident phenomena. In particular, this allowed NRC viewpoints on various phenomenological issues, as discussed in Appendix 1 of GL 88-20, to be evaluated in a straight forward manner

  12. 肠道病毒71型疫苗的研究进展%Research progress of enterovirus 71 vaccine

    Institute of Scientific and Technical Information of China (English)

    李欢

    2012-01-01

    近年来,手足口病(Hand foot mouth disease,HFMD)在中国多次爆发流行,严重威胁公众健康,尤其是5岁以下的婴幼儿.而肠道病毒71型(Enterovirus 71,EV71)是引起手足口病的主要病原体之一,由于目前还无针对该疾病有效的抗病毒药物,研制疫苗是控制EV71流行最为有效的措施.目前EV71疫苗及相关研究均取得重大突破,本文就近年来关于EV71疫苗研发、动物模型等的研究进展作一综述.

  13. The developments in the diagnosis of the enteroviruses 71%肠道病毒71型实验室诊断研究进展

    Institute of Scientific and Technical Information of China (English)

    吴星

    2012-01-01

    肠道病毒71型(Enterovirus 71,EV71)为手足口病(Hand,foot and mouth disease,HFMD)和相关疾病的主要病原体,多感染婴幼儿,少数病例可以并发呼吸道感染和心肌炎、无菌性脑膜炎、脑炎、急性弛缓性麻痹等严重疾病,可致残、致死.因此EV71实验室诊断对EV71引起疾病的治疗和防控具有重要意义.本文将从核酸检测、抗体检测及其他检测等三部分对EV71的实验室诊断方法研究进展进行了综述.

  14. 肠道病毒71型分子流行病学研究进展%Progress of Reseach on Molecular Epidemiology of Enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    田波; 段海生; 荣一兵; 周世力

    2004-01-01

    肠道病毒71型(Enterovirus type 71,EV71),自1974年首次报道以来,在世界范围内引起多次爆发与流行。EV71感染主要引起患者手足口病(hand,foot and mouth disease,HFMD),在临床上与柯萨奇病毒A16(Coxsakie A16,CA16)感染所引起的手足口病难以区别,但EV71还能够引起多种与神经系统相关的疾病。近年来,EV71病毒的流

  15. EV71 Infection and Innate Antiviral Innate Immunity%EV71的感染和抗病毒天然免疫应答

    Institute of Scientific and Technical Information of China (English)

    谢广成; 段招军

    2016-01-01

    EV71是儿童手足口病的主要病原体,部分感染儿童会出现疱疹性咽峡炎以及严重的肺水肿和/或脑干脑炎.近几年,EV71感染引起的疾病机制研究取得了诸多进展.本文针对目前EV71的敏感细胞、EV71感染激活的信号途径以及EV71感染活化的天然免疫应答或免疫逃逸机制研究进行综述,旨在为EV71抗病毒药物研制和免疫致病机理的研究提供思路.

  16. Progress in research on inactivated whole virus vaccine against enterovirus 71%EV71全病毒灭活疫苗的研究进展

    Institute of Scientific and Technical Information of China (English)

    姚昕; 毛群颖

    2012-01-01

    Hand, foot and mouth disease(HFMD) has been circulated in China and has become a serious public health problem. Enterovirus 71 (EV71) is emerged as a significant pathogen causing severe and death cases. Various EV71 vaccines are in development for controlling EV71 infection, of which inactivated vaccines are developed rapidly. At present, phase HI clinical trials will be performed on the inactivated whole virus vaccines against EV71 manufactured by three manufacturers in China. This review attempts to give an overview of the progress and quality control of inactivated whole virus vaccine against EV71.%近年来手足口病(Hand,foot and mouth disease,HFMD)在我国持续流行,已经成为危害儿童健康的严重疾病.肠道病毒71型(Enterovirus 71,EV71)感染是引起HFMD病例和婴儿重症死亡的主要病因.为控制EV71感染的流行,国内外均在开展不同种类EV71疫苗的研发,其中以灭活疫苗的进展最快,目前国内3家EV71全病毒灭活疫苗即将进入Ⅲ期临床试验阶段.本文就全病毒灭活疫苗的研发进展及质量控制和评价要点作一综述.

  17. EV71 infection correlates with viral IgG preexisting at pharyngolaryngeal mucosa in children

    Institute of Scientific and Technical Information of China (English)

    Jingchang; Xue; Yaoming; Li; Xiaoyi; Xu; Jie; Yu; Hu; Yan; Huimin; Yan

    2015-01-01

    Enterovirus 71(EV71) infection causes severe central nervous system damage, particularly for children under the age of 5 years old, which remains a major public health burden worldwide. Clinical data released that children may be repeatedly infected by different members in enterovirus and get even worsen. Mucosa, especially epithelium of alimentary canal, was considered the primary site of EV71 infection. It has been elusive whether the preexsiting viral antibody in mucosa plays a role in EV71 infection. To answer this question, we respectively measured viral antibody response and EV71 RNA copy number of one hundred throat swab specimens from clinically confirmed EV71-infected children. The results released that low-level of mucosal Ig G antibody against EV71 broadly existed in young population. More importantly, it further elucidated that the children with mucosal preexsiting EV71 Ig G were prone to be infected, which suggested a former viral Ig G mediated enhancement of viral infection in vivo.

  18. Recent Progress towards Novel EV71 Anti-Therapeutics and Vaccines.

    Science.gov (United States)

    Ng, Qingyong; He, Fang; Kwang, Jimmy

    2015-12-01

    Enterovirus 71 (EV71) is a group of viruses that belongs to the Picornaviridae family, which also includes viruses such as polioviruses. EV71, together with coxsackieviruses, is widely known for its association with Hand Foot Mouth Disease (HFMD), which generally affects children age five and below. Besides HFMD, EV71 can also trigger more severe and life-threatening neurological conditions such as encephalitis. Considering the lack of a vaccine and antiviral drug against EV71, together with the increasing spread of these viruses, the development of such drugs and vaccines becomes the top priority in protecting our younger generations. This article, hence, reviews some of the recent progress in the formulations of anti-therapeutics and vaccine generation for EV71, covering (i) inactivated vaccines; (ii) baculovirus-expressed vaccines against EV71; (iii) human intravenous immunoglobulin (IVIg) treatment; and (iv) the use of monoclonal antibody therapy as a prevention and treatment for EV71 infections.

  19. EV71-infected CD14(+) cells modulate the immune activity of T lymphocytes in rhesus monkeys.

    Science.gov (United States)

    Wang, Jingjing; Pu, Jing; Huang, Hongtai; Zhang, Ying; Liu, Longding; Yang, Erxia; Zhou, Xiaofang; Ma, Na; Zhao, Hongling; Wang, Lichun; Xie, Zhenfeng; Tang, Donghong; Li, Qihan

    2013-07-01

    Preliminary studies of the major pathogen enterovirus 71 (EV71), a member of the Picornaviridae family, have suggested that EV71 may be a major cause of fatal hand, foot and mouth disease cases. Currently, the role of the pathological changes induced by EV71 infection in the immunopathogenic response remains unclear. Our study focused on the interaction between this virus and immunocytes and indicated that this virus has the ability to replicate in CD14(+) cells. Furthermore, these EV71-infected CD14(+) cells have the capacity to stimulate the proliferation of T cells and to enhance the release of certain functional cytokines. An adaptive immune response induced by the back-transfusion of EV71-infected CD14(+) cells was observed in donor neonatal rhesus monkeys. Based on these observations, the proposed hypothesis is that CD14(+) cells infected by the EV71 virus might modulate the anti-EV71 adaptive immune response by inducing simultaneous T-cell activation.

  20. Elevated cerebrospinal fluid endothelin 1 associated with neurogenic pulmonary edema in children with enterovirus 71 encephalitis

    Directory of Open Access Journals (Sweden)

    Yi-Fang Tu

    2015-05-01

    Conclusion: ET-1 in the central nervous system may play a role in the development of NPE in children with EV71 infection and could be used as a biomarker or therapeutic target for NPE in EV71 encephalitis.

  1. Surveillance of the enterovirus type 71 in Shenzhen in 2000%2000年深圳市肠道病毒71型的监测

    Institute of Scientific and Technical Information of China (English)

    何雅青; 杨洪; 丰素娟; 严志平

    2003-01-01

    @@ 肠道病毒71型(enterovirus type 71; EV 71)的感染可导致多种临床症状,EV 71可以引起发热性疾病、手足口病、疱疹性咽峡炎、无菌性脑膜炎、脑炎、脑干脑炎、肺水肿、脊髓灰质炎样的麻痹性疾病等多种疾病,甚至导致死亡。

  2. Biological characteristics and research progress of enterovirus 71%肠道病毒71型的生物学特征及研究进展

    Institute of Scientific and Technical Information of China (English)

    戈小琴; 李鸿钧

    2010-01-01

    In recent years, the prevalence of enterovirus 71 (EV71) shows rising trend in Asia-Pacific area. The hand, foot and mouth disease caused by EV71 attracts widespread attention, and becomes a large challenge for preventive medicine and basic scientific research. This paper reviews research progress in biological characteristics, epidemiology, pathogenesis, clinical and laboratory diagnosis, prevention, and treatment of EV71.%近年来,肠道病毒71型(enterovirus 71,EV71)的流行在亚太地区呈逐渐上升趋势.EV71导致的手足口病已引起社会各界广泛关注,并成为医学预防和基础科研的挑战.此文就EV71的生物学特性、流行病学、致病机制、临床和实验室诊断、预防及治疗等方面的研究进展作一综述.

  3. RT-PCR法在快速检测肠道病毒71型(EV71)核酸中应用

    Institute of Scientific and Technical Information of China (English)

    李国兰; 郭玲; 李治悦; 卜戈; 于昌军; 张威; 杨帆; 李建国; 王伟

    2008-01-01

    @@ 2008年4月至5月发生在安徽阜阳的手足口病流行其病原已经确定为肠道病毒71型(Enterovirus 71,EV71).EV71属于小RNA病毒科(Picornaradae)肠道病毒属(Enterovirus),为一种单正链RNA病毒,病毒核酸含有大约7411个核苷酸.病毒颗粒大致呈球形,无包膜和突起,直径24~30 nm.

  4. Progress in Research on Enterovirus 71 Vaccine%肠道病毒71型疫苗的研发现状及进展

    Institute of Scientific and Technical Information of China (English)

    姚昕; 毛群颖

    2011-01-01

    Hand foot mouth disease (HFMD) has been epidemic in China in recent years. Severe and fatal cases caused by enterovirus 71 (EV71) has become the serious public healthy problem. The only effective measure to control HFMD is developing EV71 vaccine. At present, the vaccine is in developing in several manufacturers in China. This review attempts to give an overview of the necessity, feasibility as well as problem and progress in development of EV71 vaccine.%近年来,手足口病(Hand foot mouth disease,HFMD)在我国呈持续流行态势.其中,肠道病毒71型(Enterovirus 71,EV71)感染引起的严重和死亡病例已成为政府和社会高度关注的公共卫生问题.研发疫苗是控制该病流行的唯一有效措施.目前,我国已有多个单位开展了EV71疫苗的研发工作,本文就EV71疫苗研发的必要性、可行性、研发的难点及研发进展作一综述.

  5. 肠道病毒71型致病机制研究进展%Advances in the research of pathogenesis of enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    鲁卫卫; 郭会杰

    2013-01-01

    Enterovirus 71 (EV71) is one of the major pathogens of hand,foot and mouth disease,which can cause a variety of nervous system-related diseases with high disability and mortality rates.This review summarizes the recent research progress of the immune responses against EV71 and pathogenesis of EV71 infection.%肠道病毒71型(enterovirus 71,EV71)是引起手足口病的主要病原体之一,其感染可导致多种与神经系统相关的疾病,致残率及病死率较高.目前,关于其免疫机制和致病机制尚未完全清楚.此文阐述了机体对EV71的免疫应答以及EV71感染的致病机制的最新研究进展.

  6. 重症手足口病EV71感染护理体会%Nursing Experience of HFMD Children with Severe EV71 Infection

    Institute of Scientific and Technical Information of China (English)

    金利萍; 房彤; 芦玮玮

    2009-01-01

    目的:总结重症EV71感染患儿的护理经验.方法:时100多例重症EV71感染患儿的临床表现、救治措施及护理方法进行分析.结果:总结出重症手足口病EV71感染患儿各项护理要点.结论:重症EV71感染患儿的救治工作在配合护理措施后提高救治成功率.

  7. Delivery of human EV71 receptors by adeno-associated virus increases EV71 infection-induced local inflammation in adult mice.

    Science.gov (United States)

    Hsiao, Hung-Bo; Chou, Ai-Hsiang; Lin, Su-I; Lien, Shu-Pei; Liu, Chia-Chyi; Chong, Pele; Chen, Chih-Yeh; Tao, Mi-Hua; Liu, Shih-Jen

    2014-01-01

    Enterovirus71 (EV71) is now recognized as an emerging neurotropic virus in Asia and one major causative agent of hand-foot-mouth diseases (HFMD). However potential animal models for vaccine development are limited to young mice. In this study, we used an adeno-associated virus (AAV) vector to introduce the human EV71 receptors P-selectin glycoprotein ligand-1 (hPSGL1) or a scavenger receptor class-B member-2 (hSCARB2) into adult ICR mice to change their susceptibility to EV71 infection. Mice were administered AAV-hSCARB2 or AAV-hPSGL1 through intravenous and oral routes. After three weeks, expression of human SCARB2 and PSGL1 was detected in various organs. After infection with EV71, we found that the EV71 viral load in AAV-hSCARB2- or AAV-hPSGL1-transduced mice was higher than that of the control mice in both the brain and intestines. The presence of EV71 viral particles in tissues was confirmed using immunohistochemistry analysis. Moreover, inflammatory cytokines were induced in the brain and intestines of AAV-hSCARB2- or AAV-hPSGL1-transduced mice after EV71 infection but not in wild-type mice. However, neurological disease was not observed in these animals. Taken together, we successfully infected adult mice with live EV71 and induced local inflammation using an AAV delivery system.

  8. 肠道病毒71型抵抗Ⅰ型干扰素诱导的抗病毒作用%Human enterovirus 71 ( EV71 ) resisted type I interferon induced antiviral effect

    Institute of Scientific and Technical Information of China (English)

    崔雨; 宋娟; 宋芹芹; 孙鹏; 甘星; 李公启; 王克霞; 韩俊

    2012-01-01

    目的 研究肠道病毒71型(EV71)抵抗Ⅰ型干扰素(interferon,IFN)诱导的抗病毒作用.方法 将1000 U/ml的Ⅰ型干扰素(α,β)加入HeLa细胞后,去除上清中的干扰素,感染带有GFP的重组单纯疱疹病毒(HSV-1)和EV71,观察GFP的表达和PCR检测HSV-1核酸,判断Ⅰ型干扰素对HSV-1的作用.通过RT-PCR方法检测EV71 2A基因的表达从而判断EV71病毒的复制能力.结果 Ⅰ型干扰素(α,β)诱导HeLa细胞产生抗病毒蛋白而有效地抑制重组HSV-1 GFP的表达和核酸扩增.而EV71 2A的RT-PCR结果证实EV71可在Ⅰ型干扰素(α,β)作用后的HeLa细胞中有效增殖.结论 Ⅰ型干扰素(a,β)诱导产生抗病毒蛋白;EV71可在Ⅰ型干扰素(α,β)诱导产生抗病毒蛋白的HeLa细胞中有效增殖.%Objective To investigate human enterovirus 71 (EV71) resistance to type Ⅰ interferon induced antiviral effect.Methods After type Ⅰ interferons (α,β ) were incubated with HeLa cells,recombinant type Ⅰ herpes simple virus ( HSV-1 ) with green fluorescent protein (GFP) was inoculated onto the HeLa cells.HSV-1 proliferation was observed by GFP expression and PCR.After EV71 was inoculated onto HeLa cells incubated with the same quantity of interferon,proliferation of EV71 were detected by RTPCR of 2A gene. Results Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type Ⅰ interferon (α,β).However,EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR.Conclusion HeLa cell irritated by type Ⅰ interferon (α,β) produced antiviral substance that inhibits HSV-1 proliferation.EV71 resisted the antiviral substance induced by type Ⅰ interferon and could significantly replicate in the HeLa cells.

  9. Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein

    OpenAIRE

    Kiener Tanja K; Jia Qiang; Lim Xiao; He Fang; Meng Tao; Kwong Chow Vincent; Kwang Jimmy

    2012-01-01

    Abstract Background Enterovirus 71 (EV71) has emerged as a major causative agent of hand, foot and mouth disease in the Asia-Pacific region over the last decade. Hand, foot and mouth disease can be caused by different etiological agents from the enterovirus family, mainly EV71 and coxsackieviruses, which are genetically closely related. Nevertheless, infection with EV71 may occasionally lead to high fever, neurologic complications and the emergence of a rapidly fatal syndrome of pulmonary ede...

  10. New introductions of enterovirus 71 subgenogroup C4 strains, France, 2012.

    Science.gov (United States)

    Schuffenecker, Isabelle; Henquell, Cécile; Mirand, Audrey; Coste-Burel, Marianne; Marque-Juillet, Stéphanie; Desbois, Delphine; Lagathu, Gisèle; Bornebusch, Laure; Bailly, Jean-Luc; Lina, Bruno

    2014-08-01

    In France during 2012, human enterovirus 71 (EV-A71) subgenogroup C4 strains were detected in 4 children hospitalized for neonatal fever or meningitis. Phylogenetic analysis showed novel and independent EV-A71 introductions, presumably from China, and suggested circulation of C4 strains throughout France. This observation emphasizes the need for monitoring EV-A71 infections in Europe. PMID:25061698

  11. 肠道病毒71型临床研究进展

    Institute of Scientific and Technical Information of China (English)

    赵红; 欧蔚妮; 闫杰; 谢雯

    2008-01-01

    @@ 肠道病毒71型(enterovirus 71, EV71)为小RNA病毒科肠道病毒属成员.EV71于1969年首次从美国加利福尼亚州一名患有中枢神经系统疾病婴儿的粪便标本中分离出后[1],其流行范围遍及全球.

  12. A new treatment for neurogenic inflammation caused by EV71 with CR2-targeted complement inhibitor

    OpenAIRE

    Qiu Shaofu; Liu Nan; Jia Leili; Yang Guang; Su Wenli; Li Jing; Song Lixue; Yang Chaojie; Wang Jian; Zhang Chuanfu; Wang Zhongqiang; Qiao Fei; Tomlinson Stephen; Atkinson Carl; Sun Yansong

    2012-01-01

    Abstract Background Enterovirus 71 (EV71), one of the most important neurotropic EVs, has caused death and long-term neurological sequelae in hundreds of thousands of young children in the Asia-Pacific region in the past decade. The neurological diseases are attributed to infection by EV71 inducing an extensive peripheral and central nervous system (CNS) inflammatory response with abnormal cytokine production and lymphocyte depletion induced by EV71 infection. In the absence of specific antiv...

  13. 22 CFR 71.11 - Short-term full diet program.

    Science.gov (United States)

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Short-term full diet program. 71.11 Section 71.... Nationals Incarcerated Abroad § 71.11 Short-term full diet program. (a) Eligibility criteria. A prisoner is considered eligible for the short-term full diet program under the following general criteria: (1)...

  14. 41 CFR 301-71.1 - What is the purpose of an agency travel accounting system?

    Science.gov (United States)

    2010-07-01

    ... an agency travel accounting system? 301-71.1 Section 301-71.1 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES AGENCY RESPONSIBILITIES 71... accounting system? To: (a) Pay authorized and allowable travel expenses of employees; (b) Provide...

  15. 21 CFR 71.37 - Exemption of color additives for investigational use.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Exemption of color additives for investigational use. 71.37 Section 71.37 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVE PETITIONS Administrative Action on Petitions § 71.37 Exemption of color additives for investigational use. (a)...

  16. 21 CFR 71.15 - Confidentiality of data and information in color additive petitions.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Confidentiality of data and information in color additive petitions. 71.15 Section 71.15 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVE PETITIONS General Provisions § 71.15 Confidentiality of data and information in color...

  17. 48 CFR 1852.247-71 - Protection of the Florida manatee.

    Science.gov (United States)

    2010-10-01

    ... manatee. 1852.247-71 Section 1852.247-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND... and Clauses 1852.247-71 Protection of the Florida manatee. As prescribed in 1847.7001, insert the following clause: Protection of the Florida Manatee (MAR 1989) (a) Pursuant to the Endangered Species Act...

  18. 48 CFR 352.237-71 - Crime Control Act-reporting of child abuse.

    Science.gov (United States)

    2010-10-01

    ...-reporting of child abuse. 352.237-71 Section 352.237-71 Federal Acquisition Regulations System HEALTH AND... Clauses 352.237-71 Crime Control Act—reporting of child abuse. As prescribed in 337.103-70(b), the Contracting Officer shall insert the following clause: Crime Control Act of 1990—Reporting of Child...

  19. Evolutionary trajectory of the VP1 gene of human enterovirus 71 genogroup B and C viruses

    NARCIS (Netherlands)

    S.M.G. van der Sanden (Sabine); H.G.A.M. van der Avoort (Harrie); P. Lemey (Philippe); G. Uslu (Gökhan); M.P.G. Koopmans D.V.M. (Marion)

    2010-01-01

    textabstractFrom 1963 to 1986, human enterovirus 71 (HEV71) infections in the Netherlands were successively caused by viruses of subgenogroups B0, B1 and B2. A genogroup shift occurred in 1987, after which viruses of subgenogroups C1 and C2 were detected exclusively. This is in line with HEV71 typin

  20. 40 CFR 71.13 - Enforceable commitments for further actions addressing Greenhouse Gases (GHGs)

    Science.gov (United States)

    2010-07-01

    ... actions addressing Greenhouse Gases (GHGs) 71.13 Section 71.13 Protection of Environment ENVIRONMENTAL... § 71.13 Enforceable commitments for further actions addressing Greenhouse Gases (GHGs) (a) Definitions. (1) Greenhouse Gases (GHGs) means the air pollutant as defined in § 86.1818-12(a) of this chapter...

  1. 42 CFR 71.48 - Carriers in intercoastal and interstate traffic.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Carriers in intercoastal and interstate traffic. 71... Inspection § 71.48 Carriers in intercoastal and interstate traffic. Carriers, on an international voyage, which are in traffic between U.S. ports, shall be subject to inspection as described in §§ 71.31 and...

  2. Expression and Purification of Enterovirus Type 71 Polyprotein P1 using Pichia pastoris system

    Institute of Scientific and Technical Information of China (English)

    Xue Han; Xiaoling Ying; Hao Huang; Shili Zhou; Qi Huang

    2012-01-01

    Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year; However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 pretein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.

  3. 41 CFR 301-71.101 - What travel may we authorize?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What travel may we authorize? 301-71.101 Section 301-71.101 Public Contracts and Property Management Federal Travel Regulation... REQUIREMENTS Travel Authorization § 301-71.101 What travel may we authorize? You may authorize only...

  4. 7 CFR 201.71 - Establishing the source of all classes of certified seed.

    Science.gov (United States)

    2010-01-01

    ... seed. 201.71 Section 201.71 Agriculture Regulations of the Department of Agriculture (Continued... (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Certified Seed § 201.71 Establishing the source of all classes of certified seed. The certifying agency shall have evidence of the class and source...

  5. 48 CFR 1552.214-71 - Contract award-other factors-formal advertising.

    Science.gov (United States)

    2010-10-01

    ... factors-formal advertising. 1552.214-71 Section 1552.214-71 Federal Acquisition Regulations System... Provisions and Clauses 1552.214-71 Contract award—other factors—formal advertising. As prescribed in 1514.201... Advertising” (FAR 52.214-10), and “Contract Award—Construction” (FAR 52.214-19). All other...

  6. 50 CFR 71.11 - Opening of national fish hatchery areas to fishing.

    Science.gov (United States)

    2010-10-01

    ... fishing. 71.11 Section 71.11 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) MANAGEMENT OF FISHERIES CONSERVATION AREAS HUNTING AND FISHING ON NATIONAL FISH HATCHERY AREAS Fishing § 71.11 Opening of national fish hatchery areas to fishing. National...

  7. 49 CFR 392.71 - Radar detectors; use and/or possession.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Radar detectors; use and/or possession. 392.71 Section 392.71 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR... DRIVING OF COMMERCIAL MOTOR VEHICLES Prohibited Practices § 392.71 Radar detectors; use and/or...

  8. 41 CFR 301-71.100 - What is the purpose of the travel authorization process?

    Science.gov (United States)

    2010-07-01

    ... the travel authorization process? 301-71.100 Section 301-71.100 Public Contracts and Property...-AGENCY TRAVEL ACCOUNTABILITY REQUIREMENTS Travel Authorization § 301-71.100 What is the purpose of the travel authorization process? The purpose is to: (a) Provide the employee information regarding...

  9. 48 CFR 504.602-71 - Federal Procurement Data System-Public access to data.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Federal Procurement Data System-Public access to data. 504.602-71 Section 504.602-71 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION GENERAL ADMINISTRATIVE MATTERS Contract Reporting 504.602-71 Federal Procurement Data System—Public access to data....

  10. 10 CFR 71.64 - Special requirements for plutonium air shipments.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Special requirements for plutonium air shipments. 71.64... MATERIAL Package Approval Standards § 71.64 Special requirements for plutonium air shipments. (a) A package for the shipment of plutonium by air subject to § 71.88(a)(4), in addition to satisfying...

  11. 42 CFR 71.45 - Food, potable water, and waste: U.S. seaports and airports.

    Science.gov (United States)

    2010-10-01

    ... airports. 71.45 Section 71.45 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Inspection § 71.45 Food, potable water, and waste: U.S. seaports and airports. (a) Every seaport and airport... or aircraft at any seaport or airport intended for human consumption thereon shall be obtained...

  12. 48 CFR 945.603-71 - Disposal of radioactively contaminated personal property.

    Science.gov (United States)

    2010-10-01

    ... Contractor Inventory 945.603-71 Disposal of radioactively contaminated personal property. Special procedures regarding the disposal of radioactively contaminated property may be found at 41 CFR 109-45.50. ... contaminated personal property. 945.603-71 Section 945.603-71 Federal Acquisition Regulations System...

  13. 肠道病毒71型灭活疫苗相关研究现状%Current status of research of inactivated enterovirus 71 vaccines

    Institute of Scientific and Technical Information of China (English)

    杨二霞

    2013-01-01

    Hand-foot-and-mouth disease (HFMD) is a life-threatening infectious disease of children under 5 years old.In recent years,major outbreaks of HFMD caused by enterovirus 71 (EV71) have emerged in the Asia-Pacific region,and have resulted in millions of cases and hundreds of deaths each year in China since 2008.Because EV71 is one of the main pathogens of HFMD resulting in severe diseases and death,the pathogenesis of HFMD is still unclear,and no effective therapeutic drugs for EV71 infection are currently available,development of vaccines against EV71 is urgently needed.This review summarizes research progress of inactivated EV71 vaccines.%手足口病是一种威胁5岁以下儿童生命健康的传染病,近年来已在亚太地区造成了多次上百万患者发病的大规模暴发,仅在中国大陆,自2008年以来,每年发病人数就超过百万,死亡数百人.然而,由于肠道病毒71型(enterovirus 71,EV71)是导致重症和死亡病例的主要病原体,而其致病机制尚不清楚,目前尚无有效的治疗性药物,因此EV71疫苗研究成为迫切需要解决的问题.此文就EV71灭活疫苗研究进展进行综述.

  14. Rupintrivir is a promising candidate for treating severe cases of Enterovirus-71 infection

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    AIM:To evaluate the suitability of rupintrivir against Enterovirus 71(EV71)induced severe clinical symptoms using computational methods. METHODS:The structure of EV71 3C protease was predicted by homology modeling.The binding free energies between rupintrivir and EV71 3C and human rhinovirus 3C protease were computed by molecular dynamics and molecular mechanics Poisson-Boltzmann/ surface area and molecular mechanics generalized-born/ surface area methods.EV71 3C fragments obtained from clinical samples col...

  15. Genetic and antigenic characterization of enterovirus 71 in Ho Chi Minh City, Vietnam, 2011.

    Directory of Open Access Journals (Sweden)

    Le Phan Kim Thoa

    Full Text Available Enterovirus 71 (EV71 frequently causes fatal infections in young children in Asia. In 2011, EV71 epidemics occurred in southern Vietnam. We conducted genetic and antigenic analysis of the EV71 isolates and found that 94% of them were genotype C4a related to two lineages circulating in China and 6% were genotype C5 which have circulated in Vietnam since 2003. Antigenic variants were not detected. EV71 vaccines are being developed. Longitudinal enterovirus surveillance data are critical to formulate vaccination policy in Vietnam.

  16. Effects of aquifer storage and recovery activities on water quality in the Little Arkansas River and Equus Beds Aquifer, south-central Kansas, 2011-14

    Science.gov (United States)

    Stone, Mandy L.; Garrett, Jessica D.; Poulton, Barry C.; Ziegler, Andrew C.

    2016-07-18

    The Equus Beds aquifer in south-central Kansas is aprimary water source for the city of Wichita. The Equus Beds aquifer storage and recovery (ASR) project was developed to help the city of Wichita meet increasing current (2016) and future water demands. The Equus Beds ASR project pumps water out of the Little Arkansas River during above-base flow conditions, treats it using drinking-water quality standards as a guideline, and recharges it into the Equus Beds aquifer for later use. Phase II of the Equus Beds ASR project currently (2016) includes a river intake facility and a surface-water treatment facility with a 30 million gallon per day capacity. Water diverted from the Little Arkansas River is delivered to an adjacent presedimentation basin for solids removal. Subsequently, waste from the surface-water treatment facility and the presedimentation basin is returned to the Little Arkansas River through a residuals return line. The U.S. Geological Survey, in cooperation with the city of Wichita, developed and implemented a hydrobiological monitoring program as part of the ASR project to characterize and quantify the effects of aquifer storage and recovery activities on the Little Arkansas River and Equus Beds aquifer water quality.Data were collected from 2 surface-water sites (one upstream and one downstream from the residuals return line), 1 residuals return line site, and 2 groundwater well sites (each having a shallow and deep part): the Little Arkansas River upstream from the ASR facility near Sedgwick, Kansas (upstream surface-water site 375350097262800), about 0.03 mile (mi) upstream from the residuals return line site; the Little Arkansas River near Sedgwick, Kans. (downstream surface-water site 07144100), about 1.68 mi downstream from the residuals return line site; discharge from the Little Arkansas River ASR facility near Sedgwick, Kansas (residuals return line site 375348097262800); 25S 01 W 07BCCC01 SMW–S11 near CW36 (MW–7 shallow groundwater well

  17. Detection of RNA of EV71 in hospital sewage%医院污水中EV71病毒核酸的检验

    Institute of Scientific and Technical Information of China (English)

    王华洪; 白志永

    2009-01-01

    目的 研究医院污水EV71病毒核酸的检测方法,为手足口病控制提供依据.方法 用栽阳电荷的硅胶滤材吸附浓集污水中的EV71病毒,然后用RT-PCR和实时荧光PCR技术检测EV71病毒.结果 建立的栽阳电荷硅胶浓集方法能有效浓集医院污水中的EV71病毒,通过对6家医院的医院污水检测有2家检出EV71病毒核酸,所有检出EV71核酸的医院污水余氯都不符合国家卫生标准.结论 医院污水中含有EV71病毒核酸,提示其为环境中EV71病毒的重要污染源.因此需要加强医院污水消毒的监督检查.

  18. The Role of Lipid Rafts in the Early Stage of Enterovirus 71 Infection

    Directory of Open Access Journals (Sweden)

    Yong-Zhe Zhu

    2015-02-01

    Full Text Available Background/Aims: Although it has been widely accepted that Enterovirus 71 (EV71 enters permissive cells via receptor-mediated endocytosis, the details of entry mechanism for EV71 still need more exploration. This study aimed to investigate the role of lipid rafts in the early stage of EV71 Infection. Methods: The effect of cholesterol depletion or addition of exogenous cholesterol was detected by immunofluorescence assays and quantitative real-time PCR. Effects of cholesterol depletion on the association of EV71 with lipid rafts were determined by flow cytometry and co-immunoprecipitation assays. Localization and internalization of EV71 and its receptor were assayed by confocal microscpoy and sucrose gradient analysis. The impact of cholesterol on the activation of phosphoinositide 3'-kinase/Akt signaling pathway during initial virus infection was analyzed by Western-blotting. Results: Disruption of membrane cholesterol by a pharmacological agent resulted in a significant reduction in the infectivity of EV71. The inhibitory effect could be reversed by the addition of exogenous cholesterol. Cholesterol depletion post-infection did not affect EV71 infection. While virus bound equally to cholesterol-depleted cells, EV71 particles failed to be internalized by cholesterol-depleted cells. EV71 capsid protein co-localized with cholera toxin B, a lipid-raft-dependent internalization marker. Conclusion: Lipid rafts play a critical role in virus endocytosis and in the activation of PI3K/Akt signaling pathway in the early stage of EV71 infection.

  19. Determinants of EV71 immunogenicity and protection against lethal challenge in a mouse model.

    Science.gov (United States)

    Chang, Junliang; Li, Jingliang; Wei, Wei; Liu, Xin; Liu, Guanchen; Yang, Jiaxin; Zhang, Wenyan; Yu, Xiao-Fang

    2015-07-01

    Circulating enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) is a major public health problem in the Asian-Pacific region. An EV71 vaccine for HFMD prevention is currently being developed. However, viral determinants that could influence the vaccine's efficacy have not been well characterized. In this study, we isolated and characterized several EV71 strains that are currently circulating in northern and southern China. We determined that VP1 variation is a major determinant of EV71 immunogenicity. A single amino acid variation in VP1 can lead to significant differences in the breadth and potency of immune responses against primary EV71 isolates as well as the sensitivity of EV71 to heterologous neutralizing antibody responses. We also identified EV71 strains that could induce potent immunogenic and cross-neutralizing antibody responses against diverse EV71 strains. Furthermore, these neutralizing antibodies could protect neonatal mice from lethal dose challenge with various circulating EV71 viruses. Our study provides useful information for EV71 vaccine development and evaluation.

  20. Purification technology of hand -foot -and -mouth disease EV71 vaccine%手足口病EV71疫苗纯化的工艺

    Institute of Scientific and Technical Information of China (English)

    蔺芳; 穆瑞瑞; 张俊; 张可

    2012-01-01

    @@%为提高肠道病毒71型(EV71)疫苗的纯化工艺,采用粗纯、4FF层析、离子交换层析、蔗糖密度梯度离心等方法进行疫苗的纯化.结果表明,粗纯的EV71浓缩液经4FF层析和蔗糖密度梯度离心,得到高纯度的EV71疫苗.新工艺提高了EV71的纯度,适用于规模化生产.

  1. Regional potentiometric surface of the Ozark aquifer in Arkansas, Kansas, Missouri, and Oklahoma, November 2014–January 2015

    Science.gov (United States)

    Nottmeier, Anna M.

    2015-12-21

    The Ozark aquifer, within the Ozark Plateaus aquifer system (herein referred to as the “Ozark system”), is the primary groundwater source in the Ozark Plateaus physiographic province (herein referred to as the “Ozark Plateaus”) of Arkansas, Kansas, Missouri, and Oklahoma. Groundwater from the Ozark system has historically been an important part of the water resource base, and groundwater availability is a concern in some areas; dependency on the Ozark aquifer as a water supply has caused evolving, localized issues. The construction of a regional potentiometric-surface map of the Ozark aquifer is needed to aid assessment of current and future groundwater use and availability. The regional potentiometric-surface mapping is part of the U.S. Geological Survey (USGS) Groundwater Resources Program initiative (http://water.usgs.gov/ogw/gwrp/activities/regional.html) and the Ozark system groundwater availability project (http://ar.water.usgs.gov/ozarks), which seeks to quantify current groundwater resources, evaluate changes in these resources over time, and provide the information needed to simulate system response to future human-related and environmental stresses.The Ozark groundwater availability project objectives include assessing (1) growing demands for groundwater and associated declines in groundwater levels as agricultural, industrial, and public supply pumping increases to address needs; (2) regional climate variability and pumping effects on groundwater and surface-water flow paths; (3) effects of a gradual shift to a greater surface-water dependence in some areas; and (4) shale-gas production requiring groundwater and surface water for hydraulic fracturing. Data compiled and used to construct the regional Ozark aquifer potentiometric surface will aid in the assessment of those objectives.

  2. Knockdown of dystrophin Dp71 impairs PC12 cells cycle: localization in the spindle and cytokinesis structures implies a role for Dp71 in cell division.

    Directory of Open Access Journals (Sweden)

    Marcela Villarreal-Silva

    Full Text Available The function of dystrophin Dp71 in neuronal cells remains to be established. Previously, we revealed the involvement of this protein in both nerve growth factor (NGF-induced neuronal differentiation and cell adhesion by isolation and characterization of PC12 neuronal cells with depleted levels of Dp71. In this work, a novel phenotype of Dp71-knockdown cells was characterized, which is their delayed growth rate. Cell cycle analyses revealed an altered behavior of Dp71-depleted cells, which consists of a delay in G0/G1 transition and an increase in apoptosis during nocodazole-induced mitotic arrest. Dp71 associates with lamin B1 and β-dystroglycan, proteins involved in aspects of the cell division cycle; therefore, we compared the distribution of Dp71 with that of lamin B1 and β-dystroglycan in PC12 cells at mitosis and cytokinesis by means of immunofluorescence and confocal microscopy analysis. All of these three proteins exhibited a similar immunostaining pattern, localized at mitotic spindle, cleavage furrow, and midbody. It is noteworthy that a drastic decreased staining in mitotic spindle, cleavage furrow, and midbody was observed for both lamin B1 and β-dystroglycan in Dp71-depleted cells. Furthermore, we demonstrated the interaction of Dp71 with lamin B1 in PC12 cells by immunoprecipitation and pull-down assays, and importantly, we revealed that knockdown of Dp71 expression caused a marked reduction in lamin B1 levels and altered localization of the nuclear envelope protein emerin. Our data indicate that Dp71 is a component of the mitotic spindle and cytokinesis multi-protein apparatuses that might modulate the cell division cycle by affecting lamin B1 and β-dystroglycan levels.

  3. Association of Enterovirus 71 encephalitis with the interleukin-8 gene region in Chinese children.

    Science.gov (United States)

    Li, Jian; Lin, Aiwei; Yu, Chengwen; Zhang, Zhaofang; Xu, Daoyan; Hu, Wei; Liu, Liyan; Wang, Shaoning; Nie, Xiuzhen; Sun, Wenhui; Gai, Zhongtao; Chen, Zongbo

    2015-06-01

    The study was performed in 36 Chinese patients with Enterovirus 71 (EV71) encephalitis and 141 patients with EV71-related hand, foot and mouth disease (HFMD) without encephalitis. Genotyping was determined by polymerase chain reaction- restriction fragment length polymorphism. Patients with EV71 encephalitis had a significantly higher frequency of interleukin-8 (IL-8)-251TT genotype than patients with EV71-related HFMD without encephalitis (55.6% vs 31.2%, p = 0.023). The frequency of IL-8-251T alleles was significantly higher among patients with EV71 encephalitis than in patients with EV71-related HFMD without encephalitis (72.2% vs 58.9%, odds ratio 1.8, 95% confidence interval 1.0-3.2, p = 0.038). There were significant differences in gender, age, fever days, white blood cell count, C-reactive protein and blood glucose concentration and IL-8 levels among genotypes of IL-8-251A/T in EV71-infected patients, but no significant differences in alanine or aspartate aminotransferase, creatine kinase-myocardial isozyme and cerebrospinal fluid in patients with EV71 encephalitis. These findings suggest that the IL-8-251T allele is associated with susceptibility to EV71 encephalitis in Chinese patients. PMID:25751776

  4. High sensitivity and label-free detection of Enterovirus 71 by nanogold modified electrochemical impedance spectroscopy

    Science.gov (United States)

    Wang, Fang-Yu; Li, Hsing-Yuan; Tseng, Shing-Hua; Cheng, Tsai-Mu; Chu, Hsueh-Liang; Yang, Jyh-Yuan; Chang, Chia-Ching

    2013-03-01

    Enterovirus 71 (EV71), which is the most fulminant and invasive species of enterovirus, can cause children neurologic complications and death within 2-3 days after fever and rash developed. Besides, EV71 has high sequence similarity with Coxsackie A 16 (CA16) that makes differential diagnosis difficult in clinic and laboratory. Since conventional viral diagnostic method cannot diagnose EV71 quickly and EV71 can transmit at low viral titer, the patients might delay in treatment. A quick, high sensitive, and high specific test for EV71 detection is pivotal. Electrochemical impedance spectroscopy (EIS) has been applied for detecting bio-molecules as biosensors recently. In this study, we try to build a detection platform for EV71 detection by nanogold modified EIS probe. The result shows that our probe can detect 3.6 VP1/50 μl (one EV71 particle has 60 VP1) in 3 minutes. The test can also distinguish EV71 from CA16 and lysozyme. Diagnosis of enterovirus 71 by electrochemical impedance spectroscopy has the potential to apply in clinic.

  5. 肠道病毒71型的分子流行病学现况%Molecular Epidemiological Research on Enterovirus 71

    Institute of Scientific and Technical Information of China (English)

    檀晓娟; 许文波

    2008-01-01

    目的 肠道病毒71(Enterovirus 71,EV71)是导致婴幼儿感染的重要病原之一,可引起多种疾病,具有较广的疾病谱.EV71可引起大规模的手足口病(Hand,Foot,and Mouth Disease,HFMD)爆发,也可引起脑干脑炎等神经系统感染,导致死亡.现对EV71分子流行病学研究方法和研究进展进行综述,总结和分析了EV71各种基因型/亚型毒株的进化史及进化地位,对国际上EV71各种基因型/亚型的流行现况进行了概括总结.

  6. Enterovirus 71 inhibits cellular type I interferon signaling by downregulating JAK1 protein expression.

    Science.gov (United States)

    Liu, Ying; Zhang, Zhe; Zhao, Xinghui; Yu, Rui; Zhang, Xiaopeng; Wu, Shipo; Liu, Ju; Chi, Xiangyang; Song, Xiaohong; Fu, Ling; Yu, Yingqun; Hou, Lihua; Chen, Wei

    2014-08-01

    Enterovirus 71 (EV71) infection can cause severe disease and lead to death in children. Recurring outbreaks of EV71 have been reported in several countries. Interferons (IFNs) have been used for decades to treat several types of viral infection, but have a limited ability to inhibit EV71 replication. Herein, we intend to investigate the mechanisms by which EV71 inhibits the cellular type I IFN response. In this study, MRC-5 (human embryonic lung fibroblast) or RD (human rhabdomyosarcoma) cells were infected with EV71, and then treated with or without IFN-α2b. Cells were harvested and analyzed by flow cytometry to determine the level of IFNAR1. Cell lysis were prepared to detect the levels of STAT1, STAT2, phosphorylated STAT1, phosphorylated STAT2, IFNAR1, JAK1, and TYK2 by Western blotting. The phosphorylation of STAT1 and STAT2 induced by IFN were inhibited without significant downregulation of IFNAR1 in EV71-infected cells. The EV71-induced suppression of STAT1 and STAT2 phosphorylation was not rescued by the protein tyrosine phosphatases inhibitor, and was independent of suppressor of cytokine signaling protein 1/3 levels. The phosphorylation of JAK1 and TYK2 were inhibited accompanied by EV71-induced downregulation of JAK1, which occurred at a post-transcriptional level and was proteasome independent. JAK1 expression did not decrease, and IFN-α-stimulated STAT1 and STAT2 phosphorylation were not blocked in HEK293T cells overexpressing the EV71 viral protein 2A or 3C. This study demonstrates that EV71 inhibits the cellular type I IFN antiviral pathway by downregulating JAK1, while the expression of IFNAR1 does not significantly alter in EV71-infected cells. Additionally, the EV71 viral proteins 2A and 3C do not act as antagonists of cellular type I IFN signaling. PMID:24905060

  7. Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.

    Science.gov (United States)

    Ye, Xiaohua; Fan, Chen; Ku, Zhiqiang; Zuo, Teng; Kong, Liangliang; Zhang, Chao; Shi, Jinping; Liu, Qingwei; Chen, Tan; Zhang, Yingyi; Jiang, Wen; Zhang, Linqi; Huang, Zhong; Cong, Yao

    2016-03-01

    Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3) of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2), the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection. PMID:26938634

  8. Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.

    Directory of Open Access Journals (Sweden)

    Xiaohua Ye

    2016-03-01

    Full Text Available Enterovirus 71 (EV71 is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3 of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2, the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection.

  9. Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody

    Science.gov (United States)

    Ku, Zhiqiang; Zuo, Teng; Kong, Liangliang; Zhang, Chao; Shi, Jinping; Liu, Qingwei; Chen, Tan; Zhang, Yingyi; Jiang, Wen; Zhang, Linqi; Huang, Zhong; Cong, Yao

    2016-01-01

    Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of virus-antibody complex so far. The structures reveal a bivalent binding pattern of D5 antibody across the icosahedral 2-fold axis on mature virion, suggesting that D5 binding may rigidify virions to prevent their conformational changes required for subsequent RNA release. Moreover, we also identified that the complementary determining region 3 (CDR3) of D5 heavy chain directly interacts with the extremely conserved VP1 GH-loop of EV71, which was validated by biochemical and virological assays. We further showed that D5 is indeed able to neutralize a variety of EV71 genotypes and strains. Moreover, D5 could potently confer protection in a mouse model of EV71 infection. Since the conserved VP1 GH-loop is involved in EV71 binding with its uncoating receptor, the scavenger receptor class B, member 2 (SCARB2), the broadly neutralizing ability of D5 might attribute to its inhibition of EV71 from binding SCARB2. Altogether, our results elucidate the structural basis for the binding and neutralization of EV71 by the broadly neutralizing antibody D5, thereby enhancing our understanding of antibody-based protection against EV71 infection. PMID:26938634

  10. Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection.

    Directory of Open Access Journals (Sweden)

    Yorihiro Nishimura

    Full Text Available Enterovirus 71 (EV71 is one of the major causative agents of hand, foot, and mouth disease, a common febrile disease in children; however, EV71 has been also associated with various neurological diseases including fatal cases in large EV71 outbreaks particularly in the Asia Pacific region. Recently we identified human P-selectin glycoprotein ligand-1 (PSGL-1 as a cellular receptor for entry and replication of EV71 in leukocytes. PSGL-1 is a sialomucin expressed on the surface of leukocytes, serves as a high affinity counterreceptor for selectins, and mediates leukocyte rolling on the endothelium. The PSGL-1-P-selectin interaction requires sulfation of at least one of three clustered tyrosines and an adjacent O-glycan expressing sialyl Lewis x in an N-terminal region of PSGL-1. To elucidate the molecular basis of the PSGL-1-EV71 interaction, we generated a series of PSGL-1 mutants and identified the post-translational modifications that are critical for binding of PSGL-1 to EV71. We expressed the PSGL-1 mutants in 293T cells and the transfected cells were assayed for their abilities to bind to EV71 by flow cytometry. We found that O-glycosylation on T57, which is critical for PSGL-1-selectin interaction, is not necessary for PSGL-1 binding to EV71. On the other hand, site-directed mutagenesis at one or more potential tyrosine sulfation sites in the N-terminal region of PSGL-1 significantly impaired PSGL-1 binding to EV71. Furthermore, an inhibitor of sulfation, sodium chlorate, blocked the PSGL-1-EV71 interaction and inhibited PSGL-1-mediated viral replication of EV71 in Jurkat T cells in a dose-dependent manner. Thus, the results presented in this study reveal that tyrosine sulfation, but not O-glycosylation, in the N-terminal region of PSGL-1 may facilitate virus entry and replication of EV71 in leukocytes.

  11. 肠道病毒71型疫苗研究进展%Research Progress of the Enterovirus Type 71 Vaccine

    Institute of Scientific and Technical Information of China (English)

    李燕; 刘燕敏; 梁晓峰

    2011-01-01

    近几年来,手足口病(Hand,Foot and Mouth Disease,HFMD)受到越来越多的关注.肠道病毒71型(Enterovirus Type 71,EV71)是引起HFMD的病原体之一.由于HFMD重症或死亡病例以EV71为主要病原,且该病尚无特效的治疗药物,因此EV71疫苗成为预防HFMD有效的措施.国内外已有灭活疫苗、减毒活疫苗、亚单位疫苗、病毒样颗粒和脱氧核糖核酸疫苗等多种尝试,但这些疫苗仅在动物模型中进行了评价,尚未实施临床试验.现将EV71疫苗研究进展进行综述.%Recent years, hand, foot and mouth disease(HFMD)caused more and more attention. Enterovirus type 71 (EV71)is one of pathogen caused HFMD. EV7I vaccine is an effective measure to prevent HFMD because of most severe cases and deaths caused by EV7, and there is no effective drugs so far. There are several vaccine candidates! Including inactivated whole virus vaccine?live-attenuated virus vaccine, subunit vaccine, virus-like particle and deoxyribonucleic acid vaccines, which have been trial and evaluated in animals. Clinical trial, however, has not been conducted. The research progress of EV71 vaccine was reviewed in this paper.

  12. Bacteremia caused by viridans streptococci in 71 children.

    Science.gov (United States)

    Gaudreau, C; Delage, G; Rousseau, D; Cantor, E D

    1981-12-01

    A review of the hospital records of 71 patients from whose blood viridans streptococci were isolated showed that in 13 cases the patient's illness was definitely related to the bacteremia: 4 patients had endocarditis, 3 had pneumonia, 2 had peritonitis and 1 each had meningitis, a scalp wound infection, sinusitis and otitis media. The bacteremia may have contributed to the two deaths among these 13 patients. In 45 cases the viridans streptococci may have contributed to the patient's illness: 15 patients had an infection of the lower respiratory tract and 7 an infection of the upper respiratory tract, 8 were neonates with suspected septicemia, 3 had soft tissue infections, 3 had leukemia and sepsis, and 9 had miscellaneous infections; the bacteremia was unrelated to the two deaths in this group. In another 13 cases the viridans streptococci could not be related to the patient's illness. The species most frequently isolated were Streptococcus mitis, S. sanguis II and S. MG-intermedius. The outcome of the bacteremia was generally good, even among the 11 patients not treated with antibiotics. When viridans streptococci are cultured from a single blood sample, further samples of blood and, if feasible, specimens from the associated focus of infection should be obtained for culture; further blood cultures are especially important in cases of suspected endocarditis.

  13. 71st Colloquium of the International Astronomical Union

    CERN Document Server

    Rodonò, Marcello

    1983-01-01

    IAU Colloquium No. 71 had its immediate origins in a small gathering of people interested. in the optical and UV study of flare stars which took place during the 1979 Montreal General Assembly. We recognized that a fundamental change was taking place in the study of these objects. Space-borne instruments (especially lUE and Einstein) and a new genera­ tion of ground-based equipment were having a profound effect on the range of investigations it was possible to make. To extract maximum benefit from these new possibilities it would be necessary as never before to have good communication with colleagues in other disciplines, for instance,. with atomic and solar physicists. Similarly, studies of phenomena associated with the outer atmospheres of the late-type stars could now hope to give significant insights into certain aspects of solar activity. So, in view of the wide range of backgrounds of those participating, the meeting had an unusually high proportion of invited reviews while most of the contributed pape...

  14. Ballooning osteolysis in 71 failed total ankle arthroplasties.

    Science.gov (United States)

    Singh, Gurpal; Reichard, Theresa; Hameister, Rita; Awiszus, Friedemann; Schenk, Katja; Feuerstein, Bernd; Roessner, Albert; Lohmann, Christoph

    2016-08-01

    Background and purpose - Aseptic loosening is a major cause of failure in total ankle arthroplasty (TAA). In contrast to other total joint replacements, large periarticular cysts (ballooning osteolysis) have frequently been observed in this context. We investigated periprosthetic tissue responses in failed TAA, and performed an element analysis of retrieved tissues in failed TAA. Patients and methods - The study cohort consisted of 71 patients undergoing revision surgery for failed TAA, all with hydroxyapatite-coated implants. In addition, 5 patients undergoing primary TAA served as a control group. Radiologically, patients were classified into those with ballooning osteolysis and those without, according to defined criteria. Histomorphometric, immunohistochemical, and elemental analysis of tissues was performed. Von Kossa staining and digital microscopy was performed on all tissue samples. Results - Patients without ballooning osteolysis showed a generally higher expression of lymphocytes, and CD3+, CD11c+, CD20+, and CD68+ cells in a perivascular distribution, compared to diffuse expression. The odds of having ballooning osteolysis was 300 times higher in patients with calcium content >0.5 mg/g in periprosthetic tissue than in patients with calcium content ≤0.5 mg/g (p < 0.001). Interpretation - There have been very few studies investigating the pathomechanisms of failed TAA and the cause-effect nature of ballooning osteolysis in this context. Our data suggest that the hydroxyapatite coating of the implant may be a contributory factor.

  15. Search for neutrinos from the Sun using the reaction sup 71 Ga(. nu. sub e , e sup minus ) sup 71 Ge

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, A.I.; Anosov, O.L.; Faizov, E.L.; Gavrin, V.N.; Kalikhov, A.V.; Knodel, T.V.; Knyshenko, I.I.; Kornoukhov, V.N.; Mezentseva, S.A.; Mirmov, I.N.; Ostrinsky, A.V.; Pshukov, A.M.; Revzin, N.E.; Shikhin, A.A.; Timofeyev, P.V.; Veretenkin, E.P.; Vermul, V.M.; Zatsepin, G.T. (Institute for Nuclear Research, Academy of Sciences, U.S.S.R., Moscow 117312 (USSR)); Bowles, T.J.; Cleveland, B.T.; Elliott, S.R.; O' Brien, H.A.; Wark, D.L.; Wilkerson, J.F. (Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)); Davis, R. Jr.; Lande, K. (University of Pennsylvania, Philadelphia, Pennsylvania 19104 (United States)); Cherry, M.L. (Louisiana State University, Baton Rouge, Louisiana 70803 (United States)); Kouzes, R.T. (Princeton University, Princeton, New Jersey 08544 (United States))

    1991-12-09

    A radiochemical {sup 71}Ga-{sup 71}Ge experiment using 30 tons of gallium to determine the primary flux of neutrinos from the Sun has begun operation at the Baksan Neutrino Observatory in the U.S.S.R. Assuming that the extraction efficiency for {sup 71}Ge atoms produced by solar neutrinos is the same as from natural Ge carrier, we observed the capture rate to be 20{sub {minus}20}{sup +15}(stat){plus minus}32(syst) solar neutrino units (SNU), resulting in a limit of less than 79 SNU (90% C.L.). This is to be compared with 132 SNU predicted by the standard solar model.

  16. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wenjing; Tao, Junyan; Yang, Xiaoping [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China); Yang, Zhuliang [Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201 (China); Zhang, Li; Liu, Hongsheng [Department of Academy of Sciences, Liaoning University, Shenyang 110036 (China); Wu, Kailang [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China); Wu, Jianguo, E-mail: jwu@whu.edu.cn [State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wuhan 430072 (China)

    2014-07-04

    Highlights: • Triterpenoids GLTA and GLTB display anti-EV71 activities without cytotoxicity. • The compounds prevent EV71 infection by blocking adsorption of the virus to the cells. • GLTA and GLTB bind to EV71 capsid at the hydrophobic pocket to block EV71 uncoating. • The two compounds significantly inhibit the replication of EV71 viral RNA. • GLTA and GLTB may be used as potential therapeutic agents to treat EV71 infection. - Abstract: Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential

  17. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection

    International Nuclear Information System (INIS)

    Highlights: • Triterpenoids GLTA and GLTB display anti-EV71 activities without cytotoxicity. • The compounds prevent EV71 infection by blocking adsorption of the virus to the cells. • GLTA and GLTB bind to EV71 capsid at the hydrophobic pocket to block EV71 uncoating. • The two compounds significantly inhibit the replication of EV71 viral RNA. • GLTA and GLTB may be used as potential therapeutic agents to treat EV71 infection. - Abstract: Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral activities of two Ganoderma lucidum triterpenoids (GLTs), Lanosta-7,9(11),24-trien-3-one,15;26-dihydroxy (GLTA) and Ganoderic acid Y (GLTB), against EV71 infection. The results showed that the two natural compounds display significant anti-EV71 activities without cytotoxicity in human rhabdomyosarcoma (RD) cells as evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The mechanisms by which the two compounds affect EV71 infection were further elucidated by three action modes using Ribavirin, a common antiviral drug, as a positive control. The results suggested that GLTA and GLTB prevent EV71 infection through interacting with the viral particle to block the adsorption of virus to the cells. In addition, the interactions between EV71 virion and the compounds were predicated by computer molecular docking, which illustrated that GLTA and GLTB may bind to the viral capsid protein at a hydrophobic pocket (F site), and thus may block uncoating of EV71. Moreover, we demonstrated that GLTA and GLTB significantly inhibit the replication of the viral RNA (vRNA) of EV71 replication through blocking EV71 uncoating. Thus, GLTA and GLTB may represent two potential

  18. Risk Factors for Enterovirus A71 Seropositivity in Rural Indigenous Populations in West Malaysia.

    Science.gov (United States)

    NikNadia, Nmn; Sam, I-Ching; Khaidir, Nasibah; Ngui, Romano; Lim, Yvonne A L; Goh, Xiang Ting; Choy, Seow Huey; Chan, Yoke Fun

    2016-01-01

    Enterovirus A71 (EV-A71), which is transmitted by the fecal-oral route, causes hand, foot and mouth disease and, rarely, severe neurological complications. In Malaysia, the indigenous rural community (Orang Asli) has a high prevalence of parasitic diseases due to poor sanitation, water supply and hygiene practices. This cross-sectional study compared the seroepidemiology of EV-A71 among rural Orang Asli and urban Kuala Lumpur populations in West Malaysia, and determined the risk factors associated with EV-A71 seropositivity in rural Orang Asli. Seropositive rates were determined by neutralization assay. EV-A71 seropositivity was strongly associated with increasing age in both populations. Rural Orang Asli children ≤12 years had significantly higher EV-A71 seropositivity rates than urban Kuala Lumpur children (95.5% vs 57.6%, P Malaysia.

  19. Neuronal differentiation modulates the dystrophin Dp71d binding to the nuclear matrix

    International Nuclear Information System (INIS)

    The function of dystrophin Dp71 in neuronal cells remains unknown. To approach this issue, we have selected the PC12 neuronal cell line. These cells express both a Dp71f cytoplasmic variant and a Dp71d nuclear isoform. In this study, we demonstrated by electron and confocal microscopy analyses of in situ nuclear matrices and Western blotting evaluation of cell extracts that Dp71d associates with the nuclear matrix. Interestingly, this binding is modulated during NGF-induced neuronal differentiation of PC12 cells with a twofold increment in the differentiated cells, compared to control cells. Also, distribution of Dp71d along the periphery of the nuclear matrix observed in the undifferentiated cells is replaced by intense fluorescent foci localized in Center of the nucleoskeletal structure. In summary, we revealed that Dp71d is a dynamic component of nuclear matrix that might participate in the nuclear modeling occurring during neuronal differentiation

  20. Design, synthesis, and biological evaluation of anti-EV71 agents.

    Science.gov (United States)

    Li, Peng; Yang, Bailing; Hao, Fei; Wang, Ping; He, Haiying; Huang, Lei; Zhang, Xuan; Zhang, Shengbin; Peng, Xuanjia; Yin, Ke; Hu, Jiao; Chen, Xinsheng; Gu, Zhengxian; Wang, Li; Shen, Liang; Hu, Guoping; Li, Ning; Li, Jian; Chen, Shuhui; Xiao, Wei; Wang, Zhenzhong; Guo, Qingming; Chang, Xiujuan; Zhang, Lanjun; Cai, Qixu; Lin, Tianwei

    2016-07-15

    Enterovirus 71 (EV71) is a major causative agent of hand, foot and mouth disease (HFMD), which can spread its infections to the central nervous and other systems with severe consequences. In this article, design, chemical synthesis, and biological evaluation of various anti-EV71 agents which incorporate Michael acceptors are described. Further SAR study demonstrated that lactone type of Michael acceptor provided a new lead of anti-EV71 drug candidates with high anti-EV71 activity in cell-based assay and enhanced mouse plasma stability. One of the most potent compounds (2K, cell-based anti-EV71 EC50=0.028μM), showed acceptable stability profile towards mouse plasma, which resulted into promising pharmacokinetics in mouse via IP administration. PMID:27234148