WorldWideScience

Sample records for architecture seed development

  1. Seed development and carbohydrates.

    OpenAIRE

    Wittich, P.E.

    1998-01-01

    Seeds assure the plant the onset of a next generation and a way of dispersal. They consist of endosperm and an embryo (originating from gametophytic tissue), enveloped by a seed coat (sporophytic tissue). Plants generate different types of seeds. For instance, the endosperm may either be consumed by the embryo during seed development or retained for use by the embryo during germination. Differences in timing of endosperm digestion can be illustrated with broad bean ( Vicia faba ) and Gasteria...

  2. Seed development and carbohydrates.

    NARCIS (Netherlands)

    Wittich, P.E.

    1998-01-01

    Seeds assure the plant the onset of a next generation and a way of dispersal. They consist of endosperm and an embryo (originating from gametophytic tissue), enveloped by a seed coat (sporophytic tissue). Plants generate different types of seeds. For instance, the endosperm may either be consumed by

  3. Landscape Architecture and Sustainable Development

    OpenAIRE

    Walker, Jason Brian

    2001-01-01

    The purpose of this thesis is to examine the role of sustainable development in Landscape Architecture. From reviewing the literature, a position is developed. The position is that Sustainable Development is an important issue for landscape architects and that there are reasons landscape architects have had limited success in sustainable development. The method of the thesis is derived from assessing a problem of sustainable development and landscape architecture and developing a solution t...

  4. Seed Architecture Shapes Embryo Metabolism in Oilseed Rape[W][OA

    Science.gov (United States)

    Borisjuk, Ljudmilla; Neuberger, Thomas; Schwender, Jörg; Heinzel, Nicolas; Sunderhaus, Stephanie; Fuchs, Johannes; Hay, Jordan O.; Tschiersch, Henning; Braun, Hans-Peter; Denolf, Peter; Lambert, Bart; Jakob, Peter M.; Rolletschek, Hardy

    2013-01-01

    Constrained to develop within the seed, the plant embryo must adapt its shape and size to fit the space available. Here, we demonstrate how this adjustment shapes metabolism of photosynthetic embryo. Noninvasive NMR-based imaging of the developing oilseed rape (Brassica napus) seed illustrates that, following embryo bending, gradients in lipid concentration became established. These were correlated with the local photosynthetic electron transport rate and the accumulation of storage products. Experimentally induced changes in embryo morphology and/or light supply altered these gradients and were accompanied by alterations in both proteome and metabolome. Tissue-specific metabolic models predicted that the outer cotyledon and hypocotyl/radicle generate the bulk of plastidic reductant/ATP via photosynthesis, while the inner cotyledon, being enclosed by the outer cotyledon, is forced to grow essentially heterotrophically. Under field-relevant high-light conditions, major contribution of the ribulose-1,5-bisphosphate carboxylase/oxygenase–bypass to seed storage metabolism is predicted for the outer cotyledon and the hypocotyl/radicle only. Differences between in vitro– versus in planta–grown embryos suggest that metabolic heterogeneity of embryo is not observable by in vitro approaches. We conclude that in vivo metabolic fluxes are locally regulated and connected to seed architecture, driving the embryo toward an efficient use of available light and space. PMID:23709628

  5. Seed Development and Quality in Maize Cultivars

    Directory of Open Access Journals (Sweden)

    Kazem GHASSEMI-GOLEZANI

    2011-05-01

    Full Text Available In order to evaluate seed development and quality of maize (Zea mays cultivars (DC-370, SC-500, OSSK-602 and SC-604, a split plot experiment (using R.C.B. design with three replicates was conducted in 2009 at the Research Farm of the Faculty of Agriculture, University of Tabriz, Iran. Seeds were harvested at five day intervals in eight stages. Subsequently, the quality of seed samples was determined in the laboratory. Germination percentage and seedling dry weight were enhanced, but electrical conductivity of seed leachates was reduced with increasing seed weight on mother plant. Maximum seed quality of maize cultivars was attained at the end of seed filling phase. Seed quality at earlier harvests was low, because of immaturity. Differences in maximum seedling dry weight of maize cultivars were attributed to variation in genetic constitution. It was concluded that in maize cultivars, maximum seed quality could be achieved at physiological maturity.

  6. Seed-a distributed data base architecture for global management of steam-generator inspection data

    International Nuclear Information System (INIS)

    This paper deals with a data management system - called SEED (Steam-generator Eddy-current Expert Database) for global handling of SG (steam generator) tube inspection data in nuclear power plants. The SEED integrates all stages in SG tube inspection process and supports all data such as raw eddy current data, inspection history data, SG tube information, etc. SEED implemented under client/server computing architecture for supporting LAN/WAN based graphical user interface facilities using WWW programming tools. (author)

  7. Enterprise Information Architecture for Mission Development

    Science.gov (United States)

    Dutra, Jayne

    2007-01-01

    This slide presentation reviews the concept of an information architecture to assist in mission development. The integrate information architecture will create a unified view of the information using metadata and the values (i.e., taxonomy).

  8. Seed Development and Quality in Maize Cultivars

    OpenAIRE

    Kazem GHASSEMI-GOLEZANI; Zahra TAJBAKHSH; Yaeghoob RAEY

    2011-01-01

    In order to evaluate seed development and quality of maize (Zea mays) cultivars (DC-370, SC-500, OSSK-602 and SC-604), a split plot experiment (using R.C.B. design) with three replicates was conducted in 2009 at the Research Farm of the Faculty of Agriculture, University of Tabriz, Iran. Seeds were harvested at five day intervals in eight stages. Subsequently, the quality of seed samples was determined in the laboratory. Germination percentage and seedling dry weight were enhanced, but...

  9. Using Game Development to Teach Software Architecture

    Directory of Open Access Journals (Sweden)

    Alf Inge Wang

    2011-01-01

    Full Text Available This paper describes a case study of how a game project using the XNA Game Studio from Microsoft was implemented in a software architecture course. In this project, university students have to construct and design a type of software architecture, evaluate the architecture, implement an application based on the architecture, and test this implementation. In previous years, the domain of the software architecture project has been a robot controller for navigating a maze. Robot controller was chosen as the domain for the project, as there exist several papers and descriptions on reference architectures for managing mobile robots. This paper describes the changes we had to make to introduce an XNA game development project to the software architecture course, and our experiences from running a software architecture project focusing on game development and XNA. The experiences described in this paper are based on feedback from the course staff, the project reports of the students, and a mandatory course evaluation. The evaluation shows among other things that the majority of the students preferred the game project to the robot project, that XNA was considered to be suitable platform for a software architecture project, that the students found it useful to learn XNA and C#, and that some students were carried away when developing the game in the software architecture project.

  10. PLM support to architecture based development

    DEFF Research Database (Denmark)

    Bruun, Hans Peter Lomholt

    and developing architectures can be difficult to manage, update, and maintain during development. The concept of representing product architectures in computer-based product information tools has though been central in this research, and in the creation of results. A standard PLM tool (Windchill PDMLink......©) is applied for representing a model of a product architecture, and for enabling fast, precise, and safe data transfer, as well as reducing the effort to replicate and modify information. This PhD thesis describes research into the phenomena of developing products based on architectures and how to represent......Designers doing product architecture based development look to convert desired behaviour to solutions for a portfolio of products, and through modularisation pursue commonality among different variants without increasing the internal task proportional to handling variety. To develop product...

  11. Tourism, Architecture and Sustainable Development

    OpenAIRE

    Delia Popescu

    2005-01-01

    The problems of environmental damage, economic unsustainability and social dislocation caused by rapid and extensive places for recreation growth might suggest that the key to greater sustainability lies in setting limits to urbanization and changing the rules in architecture and planning for tourism.

  12. Bioclimatic underground architecture: Development and principles

    OpenAIRE

    Stojić Jasmina; Stanković Danica

    2009-01-01

    The principal idea of paper lies in analyzing contemporary architectural challenges, concerning climate changes, global warming, renewable energy deficiency and population growth. The relevant examples and principles of sustainable and selfsustainable architecture development throughout history are presented. Underground structures as passive solar systems, vegetation used as insulation, ventilation and isolation are given as one of possible solutions for this global phenomenon. By studying t...

  13. Genetic analysis of seed development in Arabidopsis thaliana.

    NARCIS (Netherlands)

    Léon-Kloosterziel, K.M.

    1997-01-01

    This thesis deals with the genetic aspects of seed development in Arabidopsisthaliana. Mutants affected in several aspects of seed development and, more specifically, in seed maturation have been isolated by various selection procedures. The mutants have been analyzed genetically, physiologically,

  14. Lean Architecture for Agile Software Development

    CERN Document Server

    Coplien, James O

    2010-01-01

    More and more Agile projects are seeking architectural roots as they struggle with complexity and scale - and they're seeking lightweight ways to do it: Still seeking? In this book the authors help you to find your own path; Taking cues from Lean development, they can help steer your project toward practices with longstanding track records; Up-front architecture? Sure. You can deliver an architecture as code that compiles and that concretely guides development without bogging it down in a mass of documents and guesses about the implementation; Documentation? Even a whiteboard diagram, or a CRC

  15. Factors influencig seeds development and maturation

    OpenAIRE

    Werschallová, Markéta

    2014-01-01

    Perennial common wheat is one of the three most grown cereals in the world, along with corn and rice. In the Czech Republic, the common wheat covers approximately 32 % of seeded areas of cereals. Countless numbers of species suitable for different locations were made by their cultivation. This bachelor´s thesis „Factors influencing seeds development and maturation“ is made by compilation of knowledge gained by several authors, writing about effects of outer space and its influence on a mother...

  16. Software engineering architecture-driven software development

    CERN Document Server

    Schmidt, Richard F

    2013-01-01

    Software Engineering: Architecture-driven Software Development is the first comprehensive guide to the underlying skills embodied in the IEEE's Software Engineering Body of Knowledge (SWEBOK) standard. Standards expert Richard Schmidt explains the traditional software engineering practices recognized for developing projects for government or corporate systems. Software engineering education often lacks standardization, with many institutions focusing on implementation rather than design as it impacts product architecture. Many graduates join the workforce with incomplete skil

  17. Seed Systems Support in Kenya: Consideration for an Integrated Seed Sector Development Approach

    OpenAIRE

    Peter Munyi; Bram De Jonge

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector Development (ISSD) approach. The ISSD approach has evolved as a response to the almost exclusive focus on formal seed systems in seed sector development programs. Instead, ISSD aims to recognize and sup...

  18. Gene expression analysis of flax seed development

    Directory of Open Access Journals (Sweden)

    Sharpe Andrew

    2011-04-01

    Full Text Available Abstract Background Flax, Linum usitatissimum L., is an important crop whose seed oil and stem fiber have multiple industrial applications. Flax seeds are also well-known for their nutritional attributes, viz., omega-3 fatty acids in the oil and lignans and mucilage from the seed coat. In spite of the importance of this crop, there are few molecular resources that can be utilized toward improving seed traits. Here, we describe flax embryo and seed development and generation of comprehensive genomic resources for the flax seed. Results We describe a large-scale generation and analysis of expressed sequences in various tissues. Collectively, the 13 libraries we have used provide a broad representation of genes active in developing embryos (globular, heart, torpedo, cotyledon and mature stages seed coats (globular and torpedo stages and endosperm (pooled globular to torpedo stages and genes expressed in flowers, etiolated seedlings, leaves, and stem tissue. A total of 261,272 expressed sequence tags (EST (GenBank accessions LIBEST_026995 to LIBEST_027011 were generated. These EST libraries included transcription factor genes that are typically expressed at low levels, indicating that the depth is adequate for in silico expression analysis. Assembly of the ESTs resulted in 30,640 unigenes and 82% of these could be identified on the basis of homology to known and hypothetical genes from other plants. When compared with fully sequenced plant genomes, the flax unigenes resembled poplar and castor bean more than grape, sorghum, rice or Arabidopsis. Nearly one-fifth of these (5,152 had no homologs in sequences reported for any organism, suggesting that this category represents genes that are likely unique to flax. Digital analyses revealed gene expression dynamics for the biosynthesis of a number of important seed constituents during seed development. Conclusions We have developed a foundational database of expressed sequences and collection of plasmid

  19. Unrealized Architectural Projects in Lithuania: Historical Development

    OpenAIRE

    Indrė Gudelytė

    2012-01-01

    The “unrealized architectural projects” are the building projects, carried out under the specific design task and intended to be built in a particular place (site), though, for certain reasons and circumstances, have never been constructed. However, up to the present day, the topic of the “unbuilt” has been analyzed just episodically in literature and sources. The article touches upon the historical development of unrealized architectural works, as well as their artistic value and role within...

  20. An Information Technology Architecture for Pharmaceutical Research and Development

    OpenAIRE

    Klingler, Daniel E.; Jaffe, Marvin E.

    1990-01-01

    Rationale for and development of an information technology architecture are presented. The architectural approach described produces a technology environment that is integrating, flexible, robust, productive, and future-oriented. Issues accompanying architecture development and potential impediments to success are discussed.

  1. Architecturally Reconfigurable Development of Mobile Games

    DEFF Research Database (Denmark)

    Zhang, Weishan

    2005-01-01

    Mobile game development must face the problem of multiple hardware and software platforms, which will bring large number of variants. To cut the development and maintenance efforts, in this paper, we present an architecturally reconfigurable software product line approach to develop mobile games....... Mobile game domain variants could be handled uniformly and traced across all kinds of software assets. The architecture and configuration mechanism in our approach make optimizations that built into meta-components propagated to all product line members. We show this approach with an industrial Role-Playing-Game...

  2. Developing product families based on architectures

    DEFF Research Database (Denmark)

    Harlou, Ulf

    2006-01-01

    The subject of this PhD thesis is development of product families based on architectures. Companies are introducing more and more product variants to fulfil the market demands. These new variants add complexity to many of the processes and systems in the companies. Reuse of standard designs (i.......e. design entities) and re-use of the way new products are developed can simplify the processes and systems. Case studies show that reuse can lead to reduction of cost and time-to-market of new products. One of the means for managing reuse of standard designs within product families are architectures. This...

  3. Bioclimatic underground architecture: Development and principles

    Directory of Open Access Journals (Sweden)

    Stojić Jasmina

    2009-01-01

    Full Text Available The principal idea of paper lies in analyzing contemporary architectural challenges, concerning climate changes, global warming, renewable energy deficiency and population growth. The relevant examples and principles of sustainable and selfsustainable architecture development throughout history are presented. Underground structures as passive solar systems, vegetation used as insulation, ventilation and isolation are given as one of possible solutions for this global phenomenon. By studying the model of Nature, certain solutions imitating systems and laws of flora and fauna are given that will insure the necessary savings of non-renewable energy during the building construction and their later energy consumption.

  4. A Model for the Development of Architectural Psychology Formation in Architectural Education

    OpenAIRE

    Semra Sema UZUNOĞLU; Harun ÖZER

    2014-01-01

    This article examines the purpose, method, and outcomes of an “Architectural Psychology” program, developed to introduce new architecture students to the subject. A combination of the architectural concepts of Social Psychology, Environmental Psychology and Psychology of Perception were used in the program. Psychology of Architecture will be taught simultaneously with the Introduction to Architecture program in a student-based educational system to help students understand psychology with its...

  5. Ragnarok: An Architecture Based Software Development Environment

    DEFF Research Database (Denmark)

    Christensen, Henrik Bærbak

    the development process and its associated data, and outlining how these solutions can be supported directly in a development environment. These hypotheses are all deeply rooted in the viewpoint that the logical software architecture forms a natural and powerful framework for handling essential...... aspects of the development process. The main contributions presented in the thesis have evolved from work with two of the hypotheses: These address the problems of management of evolution, and overview, comprehension and navigation respectively. The first main contribution is the Architectural Software......The Ragnarok project is an experimental computer science project within the field of software development environments. Taking current problems in software engineering as starting point, a small set of hypotheses are proposed, outlining plausible solutions for problems concerning the management of...

  6. Genetic analysis of seed development in Arabidopsis thaliana.

    OpenAIRE

    Léon-Kloosterziel, K.M.

    1997-01-01

    This thesis deals with the genetic aspects of seed development in Arabidopsisthaliana. Mutants affected in several aspects of seed development and, more specifically, in seed maturation have been isolated by various selection procedures. The mutants have been analyzed genetically, physiologically, and morphologically. Some of the mutants are impaired in the biosynthesis or sensitivity to the plant hormone, abscisic acid (ABA). All ABA-related mutants show reduced seed dormancy, indicating the...

  7. A Systematic Way to Develop the Software Architecture based on Architecture Vision

    OpenAIRE

    Faried, Muhammad Aamir; Ilyas, Mustafa

    2010-01-01

    In the software development life cycle, changes are inevitable. Designing the architecture of the software and writing the source code does not end the software life cycle. The software system evolves as changes in the environment and requirements are incorporated in the system. If these changes are not managed properly, the architecture of the software deteriorates and leads to architecture erosion. This study is an effort to address the problem of architecture erosion and to keep the softwa...

  8. Strategic research on the development of China's seed enterprises (brief)

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    @@ The development of agricultural industry dependson the development of seed sector to a large degree. As amain means of agricultural production, seed is producedand marketed by oneself for a long tine in China. Withthe development of science and technology and the growthof market economy, seed industry has grown into the mostimportant leading sector in developed countries'agricultural industry. As China is gearing its economy andeconomic system to the world after the country's entry intothe WTO, the research on the development strategy ofChina's seed enterprises is particularly necessary.

  9. Millimeterwave Space Power Grid architecture development 2012

    Science.gov (United States)

    Komerath, Narayanan; Dessanti, Brendan; Shah, Shaan

    This is an update of the Space Power Grid architecture for space-based solar power with an improved design of the collector/converter link, the primary heater and the radiator of the active thermal control system. The Space Power Grid offers an evolutionary approach towards TeraWatt-level Space-based solar power. The use of millimeter wave frequencies (around 220GHz) and Low-Mid Earth Orbits shrinks the size of the space and ground infrastructure to manageable levels. In prior work we showed that using Brayton cycle conversion of solar power allows large economies of scale compared to the linear mass-power relationship of photovoltaic conversion. With high-temperature materials permitting 3600 K temperature in the primary heater, over 80 percent cycle efficiency was shown with a closed helium cycle for the 1GW converter satellite which formed the core element of the architecture. Work done since the last IEEE conference has shown that the use of waveguides incorporated into lighter-than-air antenna platforms, can overcome the difficulties in transmitting millimeter wave power through the moist, dense lower atmosphere. A graphene-based radiator design conservatively meets the mass budget for the waste heat rejection system needed for the compressor inlet temperature. Placing the ultralight Mirasol collectors in lower orbits overcomes the solar beam spot size problem of high-orbit collection. The architecture begins by establishing a power exchange with terrestrial renewable energy plants, creating an early revenue generation approach with low investment. The approach allows for technology development and demonstration of high power millimeter wave technology. A multinational experiment using the International Space Station and another power exchange satellite is proposed to gather required data and experience, thus reducing the technical and policy risks. The full-scale architecture deploys pairs of Mirasol sunlight collectors and Girasol 1 GW converter satellites t

  10. Effect of GA3 treatment on seed development and seed-related gene expression in grape.

    Directory of Open Access Journals (Sweden)

    Chenxia Cheng

    Full Text Available BACKGROUND: The phytohormone gibberellic acid (GA3 is widely used in the table grape industry to induce seedlessness in seeded varieties. However, there is a paucity of information concerning the mechanisms by which GAs induce seedlessness in grapes. METHODOLOGY/PRINCIPAL FINDINGS: In an effort to systematically analyze the cause of this GA3-induced seed abortion, we conducted an in depth characterization of two seeded grape cultivars ('Kyoho' and 'Red Globe', along with a seedless cultivar ('Thompson Seedless', following treatment with GA3. In a similar fashion to the seedless control, which exhibited GA3-induced abortion of the seeds 9 days after full bloom (DAF, both 'Kyoho' and 'Red Globe' seeded varieties exhibited complete abortion of the seeds 15 DAF when treated with GA3. Morphological analyses indicated that while fertilization appeared to occur normally following GA3 treatment, as well as in the untreated seedless control cultivar, seed growth eventually ceased. In addition, we found that GA3 application had an effect on redox homeostasis, which could potentially cause cell damage and subsequent seed abortion. Furthermore, we carried out an analysis of antioxidant enzyme activities, as well as transcript levels from various genes believed to be involved in seed development, and found several differences between GA3-treated and untreated controls. CONCLUSION: Therefore, it seems that the mechanisms driving GA3-induced seedlessness are similar in both seeded and seedless cultivars, and that the observed abortion of seeds may result at least in part from a GA3-induced increase in cell damage caused by reactive oxygen species, a decrease in antioxidant enzymatic activities, and an alteration of the expression of genes related to seed development.

  11. Modelling and using product architectures in mechatronic product development

    DEFF Research Database (Denmark)

    Bruun, Hans Peter Lomholt; Mortensen, Niels Henrik

    , experiences by using the architecture representation in a mechatronic development project, and the scope of using the architecture model as a skeleton for a data structure in a PLM system. The fundamental idea for planning and modeling holistic architectures is that an improved understanding of the whole...

  12. Architecture and development of olivocerebellar circuit topography

    Directory of Open Access Journals (Sweden)

    Stacey L Reeber

    2013-01-01

    Full Text Available The cerebellum has a simple tri-laminar structure that is comprised of relatively few cell types. Yet, its internal micro-circuitry is anatomically, biochemically, and functionally complex. The most striking feature of cerebellar circuit complexity is its compartmentalized topography. Each cell type within the cerebellar cortex is organized into an exquisite map; molecular expression patterns, dendrite projections, and axon terminal fields divide the medial-lateral axis of the cerebellum into topographic sagittal zones. Here, we discuss the mechanisms that establish zones and highlight how gene expression and neural activity contribute to cerebellar pattern formation. We focus on the olivocerebellar system because its developmental mechanisms are becoming clear, its topographic termination patterns are very precise, and its contribution to zonal function is debated. This review deconstructs the architecture and development of the olivocerebellar pathway to provide an update on how brain circuit maps form and function.

  13. THE CONCEPT OF MODERN DUTCH COLONIAL ARCHITECTURE TO THE DEVELOPMENT OF JAVANESE ARCHITECTURE

    OpenAIRE

    ARDIYANTO Antonius; DJUNAEDI Achmad; IKAPUTRA Suryabrata; DJADMIKA Adi

    2014-01-01

    Modern architecture colonial present in the early 20th century, when several Dutch graduate architects had begun profession in the Dutch East Indies. The architects of had understood that the development of modern architecture in the East Indies must include the local environment and climate aspects of traditional architecture that has existed in the Dutch East Indies. In this case, two famous architects, the Thomas Karsten and Henri Maclaine Pont, became a pioneers in this movement. The conc...

  14. Changes in seed water status as characterized by NMR in developing soybean seed grown under moisture stress conditions

    Energy Technology Data Exchange (ETDEWEB)

    Krishnan, P., E-mail: pkrishnan@iari.res.in; Singh, Ravender; Verma, A.P.S.; Joshi, D.K.; Singh, Sheoraj

    2014-02-21

    Highlights: • In developing soybean seeds, moisture stress resulted in more proportion of water to bound state. • These changes are further corroborated by concomitant changes in seed metabolites. • Thus there exists a moisture stress and development stage dependence of seed tissue water status. - Abstract: Changes in water status of developing seeds of Soybean (Glycine max L. Merrill.) grown under different moisture stress conditions were characterized by proton nuclear magnetic resonance (NMR)- spin–spin relaxation time (T{sub 2}). A comparison of the seed development characteristics, composition and physical properties indicated that, characteristics like seed weight, seed number/ear, rate of seed filling increased with development stages but decreased with moisture stress conditions. The NMR- spin–spin relaxation (T{sub 2}) component like bound water increased with seed maturation (40–50%) but decreased with moisture stress conditions (30–40%). The changes in seed water status to increasing levels of moisture stress and seed maturity indicates that moisture stress resulted in more proportion of water to bound state and intermediate state and less proportion of water in free-state. These changes are further corroborated by significant changes in protein and starch contents in seeds under high moisture stress treatments. Thus seed water status during its development is not only affected by development processes but also by moisture stress conditions. This study strongly indicated a clear moisture stress and development stage dependence of seed tissue water status in developing soybean seeds.

  15. Changes in seed water status as characterized by NMR in developing soybean seed grown under moisture stress conditions

    International Nuclear Information System (INIS)

    Highlights: • In developing soybean seeds, moisture stress resulted in more proportion of water to bound state. • These changes are further corroborated by concomitant changes in seed metabolites. • Thus there exists a moisture stress and development stage dependence of seed tissue water status. - Abstract: Changes in water status of developing seeds of Soybean (Glycine max L. Merrill.) grown under different moisture stress conditions were characterized by proton nuclear magnetic resonance (NMR)- spin–spin relaxation time (T2). A comparison of the seed development characteristics, composition and physical properties indicated that, characteristics like seed weight, seed number/ear, rate of seed filling increased with development stages but decreased with moisture stress conditions. The NMR- spin–spin relaxation (T2) component like bound water increased with seed maturation (40–50%) but decreased with moisture stress conditions (30–40%). The changes in seed water status to increasing levels of moisture stress and seed maturity indicates that moisture stress resulted in more proportion of water to bound state and intermediate state and less proportion of water in free-state. These changes are further corroborated by significant changes in protein and starch contents in seeds under high moisture stress treatments. Thus seed water status during its development is not only affected by development processes but also by moisture stress conditions. This study strongly indicated a clear moisture stress and development stage dependence of seed tissue water status in developing soybean seeds

  16. MS Based Imaging of Barley Seed Development

    Institute of Scientific and Technical Information of China (English)

    Manuela Peukert; Andrea Matros; Hans-Peter Mock

    2012-01-01

    Spatially resolved analysis of metabolites and proteins is essential to model compartmentalized cellular processes in plants.Within recent years,tremendous progress has been made in MS based imaging (MSI) techniques,mostly MALDI MSI.The technology has been pioneered and is now widely applied in medicinal and pharmacological studies,and in recent years found its way into plant science (Kaspar et al.,2011; Peukert etal.,2012).We are interested in the elucidation of spatially resolved metabolic networks related to barley grain development.An understanding of developmentally and ecologically regulated processes affecting agronomical traits such as final grain weight,seed quality and stress tolerance is of outmost importance,as barley provides one of the staple foods.Barley also serves as a model plant for other cereals such as wheat.The presentation will introduce an untargeted MALDI MSI approach to the analysis of me-tabolite patterns during barley grain development.We analyzed longitudinal and cross sections from developing barley grains (3,7,10 and 14 days after pollination).In the presentation we will address spatial resolution,sensitivity and identification of unknown compounds will also be discussed.A major task is to connect the metabolite patterns to distinct cellular and physiological events.As an example,particular metabolite distributions indicative for nutrient transport into the developing endosperm will be shown.

  17. Microprobing the Molecular Spatial Distribution and Structural Architecture of Feed-type Sorghum Seed Tissue (Sorghum Bicolor L.) using the Synchrotron Radiation Infrared Microspectroscopy Technique

    International Nuclear Information System (INIS)

    Sorghum seed (Sorghum bicolor L.) has unique degradation and fermentation behaviours compared with other cereal grains such as wheat, barley and corn. This may be related to its cell and cell-wall architecture. The advanced synchrotron radiation infrared microspectroscopy (SR-IMS) technique enables the study of cell or living cell biochemistry within cellular dimensions. The objective of this study was to use the SR-IMS imaging technique to microprobe molecular spatial distribution and cell architecture of the sorghum seed tissue comprehensively. High-density mapping was carried out using SR-IMS on beamline U2B at the National Synchrotron Light Source (Brookhaven National Laboratory, NY, USA). Molecular images were systematically recorded from the outside to the inside of the seed tissue under various chemical functional groups and their ratios [peaks at ∼1725 (carbonyl C=O ester), 1650 (amide I), 1657 (protein secondary structure α-helix), 1628 (protein secondary structure β-sheet), 1550 (amide II), 1515 (aromatic compounds of lignin), 1428, 1371, 1245 (cellulosic compounds in plant seed tissue), 1025 (non-structural CHO, starch granules), 1246 (cellulosic material), 1160 (CHO), 1150 (CHO), 1080 (CHO), 930 (CHO), 860 (CHO), 3350 (OH and NH stretching), 2960 (CH3 anti-symmetric), 2929 (CH2 anti-symmetric), 2877 (CH3 symmetric) and 2848 cm-1 (CH2 asymmetric)]. The relative protein secondary structure α-helix to β-sheet ratio image, protein amide I to starch granule ratio image, and anti-symmetric CH3 to CH2 ratio image were also investigated within the intact sorghum seed tissue. The results showed unique cell architecture, and the molecular spatial distribution and intensity in the sorghum seed tissue (which were analyzed through microprobe molecular imaging) were generated using SR-IMS. This imaging technique and methodology has high potential and could be used for scientists to develop specific cereal grain varieties with targeted food and feed quality, and

  18. Seed parasitism redirects ovule development in Douglas fir

    OpenAIRE

    Von Aderkas, Patrick; Rouault, Gaëlle; Wagner, Rebecca; Rohr, René; Roques, Alain

    2005-01-01

    Many parasitic species of insects complete their entire development in seeds. They feed off storage reserves within the ovule. These reserves only normally accumulate in fertilized ovules. Consequently, female insects that oviposit their eggs directly into the plant ovule need to be able to select correctly, as unfertilized ovules of conifers normally become so-called empty seed. We provide clear evidence that in conifers, seed-parasitizing insects do not need to discriminate between fertiliz...

  19. Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice

    Directory of Open Access Journals (Sweden)

    Hye-Jung Lee

    2015-06-01

    Full Text Available The major seed storage proteins (SSPs in rice seeds have been classified into three types, glutelins, prolamins, and globulin, and the proportion of each SSP varies. It has been shown in rice mutants that when either glutelins or prolamins are defective, the expression of another type of SSP is promoted to counterbalance the deficit. However, we observed reduced abundances of glutelins and prolamins in dry seeds of a globulin-deficient rice mutant (Glb-RNAi, which was generated with RNA interference (RNAi-induced suppression of globulin expression. The expression of the prolamin and glutelin subfamily genes was reduced in the immature seeds of Glb-RNAi lines compared with those in wild type. A proteomic analysis of Glb-RNAi seeds showed that the reductions in glutelin and prolamin were conserved at the protein level. The decreased pattern in glutelin was also significant in the presence of a reductant, suggesting that the polymerization of the glutelin proteins via intramolecular disulfide bonds could be interrupted in Glb-RNAi seeds. We also observed aberrant and loosely packed structures in the storage organelles of Glb-RNAi seeds, which may be attributable to the reductions in SSPs. In this study, we evaluated the role of rice globulin in seed development, showing that a deficiency in globulin could comprehensively reduce the expression of other SSPs.

  20. Development of a Precision Seed Drill for Oilseed Rape

    OpenAIRE

    Ahmadi, Ebrahim; GHASSEMZADEH, Hamid Reza; Mohammad MOGHADDAM; KIM, Kyeong Uk

    2008-01-01

    At the present time, the most widely used machine for seed and oilseed planting is a seed drill with a roller-type-metering device. Due to nonuniform spacing of seeds along the rows and lack of control on planting depth, this machine aggravates the growth related problems and degrades the quality of a sensitive crop such as oilseed rape. To solve the above-mentioned problems, an attempt was made to develop a precision seed drill with a roller-type-metering device and a depth control system. S...

  1. Aspects of the barley seed proteome during development and germination

    DEFF Research Database (Denmark)

    Finnie, Christine; Maeda, K.; Østergaard, O.;

    2004-01-01

    Analysis of the water-soluble barley seed proteome has led to the identification of proteins by MS in the major spots on two-dimensional gels covering the pi ranges 4-7 and 6-11. This provides the basis for in-depth studies of proteome changes during seed development and germination, tissue...

  2. Development of nutritious snacks by incorporation of amaranth seeds, watermelon seeds and their flour

    Directory of Open Access Journals (Sweden)

    Paul Virginia

    2014-11-01

    Full Text Available The present study was carried out with the objectives to find out the sensory acceptability, the nutrient content and cost of prepared products. The products prepared were “Biscuits”, “Mathri” and “Laddoo” by incorporation of amaranth seeds, watermelon seeds and their flour in different proportions (10:10, 20:10, and 30:10 served as treatments T1, T2 and T3 respectively T0, without incorporation of amaranth seeds, watermelon seeds and their flour served as control. The products were organoleptically evaluated by using Nine point Hedonic scale. The data obtained during study were analyzed statistically using analysis of variance and C.D techniques. The prepared products were analyzed for nutrient content using the standard method of AOAC (2005.It was concluded that in case of “Biscuits” and “Mathri” with incorporation level 20 percent amaranth seeds flour and 10 percent watermelon seeds flour scored highest while in case of “Laddoo” with incorporation level 20 percent amaranth seeds and 10 percent watermelon seeds scored highest, with regard to colour and appearance, body and texture, taste and flavour and over all acceptability, However all the treatments were found to be acceptable. It is therefore concluded that amaranth seeds and watermelon seeds can be suitably incorporated in various developed products. “Laddoo” had maximum carbohydrate (64.49g/100g, protein (13.59g/100g calcium (100.1mg/100g and iron (3.33mg/100g content. The content of Protein (14.46g/100g, carbohydrate (59.90 Calcium (59.90mg/100g, were increased as compared to control in “Biscuits”. “Mathri” was rich in Protein, Fat, carbohydrate and calcium content (11.10g/100g, 38.56g/100g, 38.83g/100g, and 53.95mg/100g. Cost of products on the basis raw ingredients per 100g ranged between Rs 6.33-12.45 for “Biscuits”, Rs 16.06-30.07, Rs 12.27-18.19 for “Mathri” and Rs.6.42-12.26 for “Laddoo”. On the basis of findings it is concluded

  3. ADaPPT: Enterprise Architecture Thinking for Information Systems Development

    Directory of Open Access Journals (Sweden)

    Hanifa Shah

    2011-01-01

    Full Text Available Enterprises have architecture: whether it is visible or invisible is another matter. An enterprises' architecture determines the way in which it works to deliver its business objectives and the way in which it can change to continue to meet its evolving business objectives. Enterprise architectural thinking can facilitate effective strategic planning and information systems development. This paper reviews enterprise architecture (EA and its concepts. It briefly considers EA frameworks. It describes the ADaPPT (Aligning Data, People, Processes and Technology EA approach as a means to managing organisational complexity and change. Future research directions are discussed.

  4. Using an Integrated Distributed Test Architecture to Develop an Architecture for Mars

    Science.gov (United States)

    Othon, William L.

    2016-01-01

    The creation of a crew-rated spacecraft architecture capable of sending humans to Mars requires the development and integration of multiple vehicle systems and subsystems. Important new technologies will be identified and matured within each technical discipline to support the mission. Architecture maturity also requires coordination with mission operations elements and ground infrastructure. During early architecture formulation, many of these assets will not be co-located and will required integrated, distributed test to show that the technologies and systems are being developed in a coordinated way. When complete, technologies must be shown to function together to achieve mission goals. In this presentation, an architecture will be described that promotes and advances integration of disparate systems within JSC and across NASA centers.

  5. Architecture design in global and model-centric software development

    NARCIS (Netherlands)

    Heijstek, Werner

    2012-01-01

    This doctoral dissertation describes a series of empirical investigations into representation, dissemination and coordination of software architecture design in the context of global software development. A particular focus is placed on model-centric and model-driven software development.

  6. Architecture-Centric Development in Globally Distributed Projects

    Science.gov (United States)

    Sauer, Joachim

    In this chapter architecture-centric development is proposed as a means to strengthen the cohesion of distributed teams and to tackle challenges due to geographical and temporal distances and the clash of different cultures. A shared software architecture serves as blueprint for all activities in the development process and ties them together. Architecture-centric development thus provides a plan for task allocation, facilitates the cooperation of globally distributed developers, and enables continuous integration reaching across distributed teams. Advice is also provided for software architects who work with distributed teams in an agile manner.

  7. [Development strategies on seed seedling of Chinese medicinal materials].

    Science.gov (United States)

    Li, Longyun; Peng, Rui; Li, Hongli; Wu, Yekuan; Cui, Guanglin

    2010-01-01

    This paper analyzed the development status, industrialization system, standardization system and new variety breeding of seed and seedling of Chinese medicinal materials. Based on the development status and problems facing to the seed and seedling industry in China, some reasonable countermeasures are proposed in this article. The main measures include strengthening new variety breeding, establishing experiment site, protecting new variety and standardizing the market and quality management. PMID:20394305

  8. Isotope labeling-based quantitative proteomics of developing seeds of castor oil seed (Ricinus communis L.)

    DEFF Research Database (Denmark)

    Nogueira, Fábio C S; Palmisano, Giuseppe; Schwämmle, Veit;

    2013-01-01

    In this study, we used a mass spectrometry-based quantification approach employing isotopic (ICPL) and isobaric (iTRAQ) labeling to investigate the pattern of protein deposition during castor oil seed (Ricinus communis L.) development, including that of proteins involved in fatty acid metabolism...... give important insights into certain aspects of the biology of castor oil seed development such as carbon flow, anabolism, and catabolism of fatty acid and the pattern of deposition of SSPs, toxins, and allergens such as ricin and 2S albumins. We also found, for the first time, some genes of SSP that...... could be mapped to extant castor gene models, considerably expanding the number of proteins so far identified from developing castor seeds. Cluster validation and statistical analysis resulted in 975 protein trend patterns and the relative abundance of 618 proteins. The results presented in this work...

  9. Exploring the Materials of Architectural Development

    OpenAIRE

    Ditcher, Kamille

    2012-01-01

    A factory inspired exploration in the architectural potential of space, material, sound, and light. This thesis is divided into chapters of the media types used for design. Primarily, the materials include photography, drawing, and modeling. The process yields overlap and interchange in ways of design that alter the ways of seeing and of advancing the work. Consequently, divisions of the book reoccur throughout the course of the thesis.

  10. Pathways for the developing Myanmar’s seed sector: A scoping study

    OpenAIRE

    Broek, van den, Wouter; A. Subedi; Jongeleen, F.; Naing Lin Oo

    2015-01-01

    The study presents an integrated assessment of Myanmar’s seed sector. The study includes information and analyses on regulatory environment for seed production and sales, a characterization of Myanmar’s seed sector with its various seed systems, a landscape of current seed sector interventions; an analysis of three seed value-chains and Myanmar’s seed farm system; as well as business opportunities for the private sector. The report concludes with a number of pathways for developing a vibrant ...

  11. A proteomic analysis of seed development in Brassica campestri L.

    Directory of Open Access Journals (Sweden)

    Wenlan Li

    Full Text Available To gain insights into the protein dynamics during seed development, a proteomic study on the developing Brassica campestri L. seeds with embryos in different embryogenesis stages was carried out. The seed proteins at 10, 16, 20, 25 and 35 DAP (days after pollination, respectively, were separated using two-dimensional gel electrophoresis and identities of 209 spots with altered abundance were determined by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS. These proteins were classified into 16 groups according to their functions. The most abundant proteins were related to primary metabolism, indicating the heavy demand of materials for rapid embryo growth. Besides, the high amount of proteins involved in protein processing and destination indicated importance of protein renewal during seed development. The remaining were those participated in oxidation/detoxification, energy, defense, transcription, protein synthesis, transporter, cell structure, signal transduction, secondary metabolism, transposition, DNA repair, storage and so on. Protein abundance profiles of each functional class were generated and hierarchical cluster analysis established 8 groups of dynamic patterns. Our results revealed novel characters of protein dynamics in seed development in Brassica campestri L. and provided valuable information about the complex process of seed development in plants.

  12. Global transcriptome analysis of developing chickpea (Cicer arietinum L. seeds

    Directory of Open Access Journals (Sweden)

    Seema ePradhan

    2014-12-01

    Full Text Available Understanding developmental processes, especially in non-model crop plants, is extremely important in order to unravel unique mechanisms regulating development. Chickpea (C. arietinum L. seeds are especially valued for their high carbohydrate and protein content. Therefore, in order to elucidate the mechanisms underlying seed development in chickpea, deep sequencing of transcriptomes from four developmental stages was undertaken. In this study, next generation sequencing platform was utilised to sequence the transcriptome of four distinct stages of seed development in chickpea. About 1.3 million reads were generated which were assembled into 51,099 unigenes by merging the de novo and reference assemblies. Functional annotation of the unigenes was carried out using the Uniprot, COG and KEGG databases. RPKM based digital expression analysis revealed specific gene activities at different stages of development which was validated using Real time PCR analysis. More than 90% of the unigenes were found to be expressed in at least one of the four seed tissues. DEGseq was used to determine differentially expressing genes which revealed that only 6.75% of the unigenes were differentially expressed at various stages. Homology based comparison revealed 17.5% of the unigenes to be putatively seed specific. Transcription factors were predicted based on HMM profiles built using TF sequences from five legume plants and analysed for their differential expression during progression of seed development. Expression analysis of genes involved in biosynthesis of important secondary metabolites suggested that chickpea seeds can serve as a good source of antioxidants. Since transcriptomes are a valuable source of molecular markers like simple sequence repeats (SSRs, about 12,000 SSRs were mined in chickpea seed transcriptome and few of them were validated. In conclusion, this study will serve as a valuable resource for improved chickpea breeding.

  13. Biochemical characterisation during seed development of oil palm (Elaeis guineensis).

    Science.gov (United States)

    Kok, Sau-Yee; Namasivayam, Parameswari; Ee, Gwendoline Cheng-Lian; Ong-Abdullah, Meilina

    2013-07-01

    Developmental biochemical information is a vital base for the elucidation of seed physiology and metabolism. However, no data regarding the biochemical profile of oil palm (Elaeis guineensis Jacq.) seed development has been reported thus far. In this study, the biochemical changes in the developing oil palm seed were investigated to study their developmental pattern. The biochemical composition found in the seed differed significantly among the developmental stages. During early seed development, the water, hexose (glucose and fructose), calcium and manganese contents were present in significantly high levels compared to the late developmental stage. Remarkable changes in the biochemical composition were observed at 10 weeks after anthesis (WAA): the dry weight and sucrose content increased significantly, whereas the water content and hexose content declined. The switch from a high to low hexose/sucrose ratio could be used to identify the onset of the maturation phase. At the late stage, dramatic water loss occurred, whereas the content of storage reserves increased progressively. Lauric acid was the most abundant fatty acid found in oil palm seed starting from 10 WAA. PMID:23575803

  14. Developing a Secure Web Service Architecture for SVG Image Delivery

    OpenAIRE

    Sabah Mohammed; Jinan Fiaidhi; Hamada Ghenniwa; Marshall Hahn

    2006-01-01

    Web Services are substantially growing and become vital for businesses and organizations. A major concern, especially for mission-critical applications is Security. This study focuses on developing Scalable Vector Graphics (SVG) as Web services. In particular, we develop a service-oriented architecture that securely manages SVG Web services using the intermediary design pattern. In the proposed architecture we introduced two kinds of specialized security intermediaries to enforce SVG signatur...

  15. FRAMEWORK FOR DEVELOPED SIMPLE ARCHITECTURE ENTERPRISE – FDSAE

    Directory of Open Access Journals (Sweden)

    Nieto Bernal Wilson

    2014-12-01

    Full Text Available In This article presents a framework for develop de Architecture enterprise based on the articulation of emerging paradigms for architecture development of information enterprise [1]. The first one comes from the agile methods and it is inspired on the Scrum model which aim to simplify the complex task of developing a quality software, the second the processes models whose are oriented the development of Architectures Enterprise as Zachman and TOGAF in a paradigm of the Model Driven and principles de reference de architecture de Software form the paradigms Generation (MDG, these approaches are integrated eventually leading to the formulation and presentation of an framework for developed simple architecture enterprise – FDSAE- The goal is to present a simple, portable, understandable terms enabling, modeling and design business information architecture in any organizational environment, in addition to this, there are important aspects related to the unified Modeling Language UML 2.5 and the Business Process Modeling BPMn that become tools to obtain the products in the FDSAE Framework, This framework is an improved version of Framework MADAIKE [2] developed by the same authors.

  16. Proteome analysis of the inner integument from developing Jatropha curcas L. seeds.

    Science.gov (United States)

    Soares, Emanoella L; Shah, Mohibullah; Soares, Arlete A; Costa, José H; Carvalho, Paulo; Domont, Gilberto B; Nogueira, Fábio C S; Campos, Francisco A P

    2014-08-01

    In this study, we performed a systematic proteomic analysis of the inner integument from developing seeds of Jatropha curcas and further explored the protein machinery responsible for generating the carbon and nitrogen sources to feed the growing embryo and endosperm. The inner integument of developing seeds was dissected into two sections called distal and proximal, and proteins were extracted from these sections and from the whole integument and analyzed using an EASY-nanoLC system coupled to an ESI-LTQ-Orbitrap Velos mass spectrometer. We identified 1526, 1192, and 1062 proteins from the proximal, distal, and whole inner integuments, respectively. The identifications include those of peptidases and other hydrolytic enzymes that play a key role in developmental programmed cell death and proteins associated with the cell-wall architecture and modification. Because many of these proteins are differentially expressed within the integument cell layers, these findings suggest that the cells mobilize an array of hydrolases to produce carbon and nitrogen sources from proteins, carbohydrates, and lipids available within the cells. Not least, the identification of several classes of seed storage proteins in the inner integument provides additional evidence of the role of the seed coat as a transient source of reserves for the growing embryo and endosperm. PMID:25010673

  17. Modelling architectures in multi-product oriented technology development

    DEFF Research Database (Denmark)

    Guðlaugsson, Tómas Vignir

    derivative products are not completely defined. Yet, decisions need to be made during technology development on the capabilities to be provided through the development to fulfil future application requirements,provide a foundation for future products, and development of a production system capable of...... producing future products and supporting technology development through prototype production.To support technology development aimed at a broad range of application requirements,two modelling frameworks are introduced: the product technology architecture modelling framework and the production architecture......This thesis investigates the use of architecture modelling in a technology development context.This context presents greater uncertainties than more mature new product development. Applications—the use of products based on the technology being developed—are not fully identified and the requirements...

  18. Architecture independent environment for developing engineering software on MIMD computers

    Science.gov (United States)

    Valimohamed, Karim A.; Lopez, L. A.

    1990-01-01

    Engineers are constantly faced with solving problems of increasing complexity and detail. Multiple Instruction stream Multiple Data stream (MIMD) computers have been developed to overcome the performance limitations of serial computers. The hardware architectures of MIMD computers vary considerably and are much more sophisticated than serial computers. Developing large scale software for a variety of MIMD computers is difficult and expensive. There is a need to provide tools that facilitate programming these machines. First, the issues that must be considered to develop those tools are examined. The two main areas of concern were architecture independence and data management. Architecture independent software facilitates software portability and improves the longevity and utility of the software product. It provides some form of insurance for the investment of time and effort that goes into developing the software. The management of data is a crucial aspect of solving large engineering problems. It must be considered in light of the new hardware organizations that are available. Second, the functional design and implementation of a software environment that facilitates developing architecture independent software for large engineering applications are described. The topics of discussion include: a description of the model that supports the development of architecture independent software; identifying and exploiting concurrency within the application program; data coherence; engineering data base and memory management.

  19. Architectures of small satellite programs in developing countries

    Science.gov (United States)

    Wood, Danielle; Weigel, Annalisa

    2014-04-01

    Global participation in space activity is growing as satellite technology matures and spreads. Countries in Africa, Asia and Latin America are creating or reinvigorating national satellite programs. These countries are building local capability in space through technological learning. This paper analyzes implementation approaches in small satellite programs within developing countries. The study addresses diverse examples of approaches used to master, adapt, diffuse and apply satellite technology in emerging countries. The work focuses on government programs that represent the nation and deliver services that provide public goods such as environmental monitoring. An original framework developed by the authors examines implementation approaches and contextual factors using the concept of Systems Architecture. The Systems Architecture analysis defines the satellite programs as systems within a context which execute functions via forms in order to achieve stakeholder objectives. These Systems Architecture definitions are applied to case studies of six satellite projects executed by countries in Africa and Asia. The architectural models used by these countries in various projects reveal patterns in the areas of training, technical specifications and partnership style. Based on these patterns, three Archetypal Project Architectures are defined which link the contextual factors to the implementation approaches. The three Archetypal Project Architectures lead to distinct opportunities for training, capability building and end user services.

  20. Developing Distributed System With Service Resource Oriented Architecture

    Directory of Open Access Journals (Sweden)

    Riyanarto Sarno

    2012-06-01

    Full Text Available Service Oriented Architecture is a design paradigm in software engineering with which a distributed system is built for an enterprise. This paradigm aims at providing the system as a service through a protocol in web service technology, namely Simple Object Access Protocol (SOAP. However, SOA is service level agreements of webservice. For this reason, this reasearch aims at combining SOA with Resource Oriented Architecture in order to expand scalability of services. This combination creates Sevice Resource Oriented Architecture (SROA with which a distributed system is developed that integrates services within project management software. Following this design, the software is developed according to a framework of Agile Model Driven Development which can reduce complexities of the whole process of software development.

  1. Architectural development of an advanced EVA Electronic System

    Science.gov (United States)

    Lavelle, Joseph

    1992-01-01

    An advanced electronic system for future EVA missions (including zero gravity, the lunar surface, and the surface of Mars) is under research and development within the Advanced Life Support Division at NASA Ames Research Center. As a first step in the development, an optimum system architecture has been derived from an analysis of the projected requirements for these missions. The open, modular architecture centers around a distributed multiprocessing concept where the major subsystems independently process their own I/O functions and communicate over a common bus. Supervision and coordination of the subsystems is handled by an embedded real-time operating system kernel employing multitasking software techniques. A discussion of how the architecture most efficiently meets the electronic system functional requirements, maximizes flexibility for future development and mission applications, and enhances the reliability and serviceability of the system in these remote, hostile environments is included.

  2. Coordination of Endoplasmic Reticulum (ER) Signaling During Maize Seed Development

    Energy Technology Data Exchange (ETDEWEB)

    Boston, Rebecca S.

    2010-11-20

    Seed storage reserves represent one of the most important sources of renewable fixed carbon and nitrogen found in nature. Seeds are well-adapted for diverting metabolic resources to synthesize storage proteins as well as enzymes and structural proteins needed for their transport and packaging into membrane bound storage protein bodies. Our underlying hypothesis is that the endoplasmic reticulum (ER) stress response provides the critical cellular control of metabolic flux required for optimal accumulation of storage reserves in seeds. This highly conserved response is a cellular mechanism to monitor the protein folding environment of the ER and restore homeostasis in the presence of unfolded or misfolded proteins. In seeds, deposition of storage proteins in protein bodies is a highly specialized process that takes place even in the presence of mutant proteins that no longer fold and package properly. The capacity of the ER to deposit these aberrant proteins in protein bodies during a period that extends several weeks provides an excellent model for deconvoluting the ER stress response of plants. We have focused in this project on the means by which the ER senses and responds to functional perturbations and the underlying intracellular communication that occurs among biosynthetic, trafficking and degradative pathways for proteins during seed development.

  3. Proteomics of seed development, desiccation tolerance, germination and vigor.

    Science.gov (United States)

    Wang, Wei-Qing; Liu, Shu-Jun; Song, Song-Quan; Møller, Ian Max

    2015-01-01

    Proteomics, the large-scale study of the total complement of proteins in a given sample, has been applied to all aspects of seed biology mainly using model species such as Arabidopsis or important agricultural crops such as corn and rice. Proteins extracted from the sample have typically been separated and quantified by 2-dimensional polyacrylamide gel electrophoresis followed by liquid chromatography and mass spectrometry to identify the proteins in the gel spots. In this way, qualitative and quantitative changes in the proteome during seed development, desiccation tolerance, germination, dormancy release, vigor alteration and responses to environmental factors have all been studied. Many proteins or biological processes potentially important for each seed process have been highlighted by these studies, which greatly expands our knowledge of seed biology. Proteins that have been identified to be particularly important for at least two of the seed processes are involved in detoxification of reactive oxygen species, the cytoskeleton, glycolysis, protein biosynthesis, post-translational modifications, methionine metabolism, and late embryogenesis-abundant (LEA) proteins. It will be useful for molecular biologists and molecular plant breeders to identify and study genes encoding particularly interesting target proteins with the aim to improve the yield, stress tolerance or other critical properties of our crop species. PMID:25461695

  4. A component Architecture for Simulator Development

    NARCIS (Netherlands)

    Kuijpers, N.H.L.; Gool, P.C.A. van; Jense, G.J.

    1998-01-01

    Research, Development and Engineering applications require the rapid development of simulators, preferably through the reuse of simulator components In order to facilitate the reuse and exchange of simulator components, research institutes and industry in The Netherlands are collaborating in the SIM

  5. The Circadian Clock-Controlled Transcriptome of Developing Soybean Seeds.

    Science.gov (United States)

    A number of metabolic and physiological processes in plants are controlled by the circadian clock, which enables the plant to anticipate daily changes in the environment. Microarray expression profiling was used to identify circadian clock controlled genes expressed in developing soybean seeds. 1.8...

  6. Physiology of tuber development and stolon architecture

    OpenAIRE

    Roumeliotis, E.

    2012-01-01

    In this thesis, we used phytohormonal applications, cloning of potato genes related to phytohormone biosynthesis and investigated the  content of several phytohormones in order to clarify how different phytohormones work together to achieve potato tuber development. Our research revealed that phytohormone auxin is important during tuber development, and possibly works together with gibberellins, another group of phytohormones, to achieve tuber initiation and tuber growth. In addition, we...

  7. Molecular characterization of a GA-inducible gene, Cvsus1, in developing watermelon seeds.

    Science.gov (United States)

    Kim, Joonyul; Jun, Sung-Hoon; Kang, Hong-Gyu; Lee, Jinwon; An, Gynheung

    2002-10-31

    To understand the molecular mechanisms that control seed development, we isolated a seed-preferential gene from ESTs of developing watermelon seeds. The gene Cvsus1 encodes a protein that is 86% identical to the Vicia faba sucrose synthase expressed in developing seeds. RNA blot analysis showed that Cvsus1 was preferentially expressed in watermelon seeds. We also investigated gene expression levels both in pollinated seeds and in parthenocarpic seeds, which lack zygotic tissues. Whereas the transcript level of Cvsus1 was rapidly increased during normal seed development, the expression was not significantly increased in the parthenocarpic seeds. However, treating the parthenocarpic fruits with GA3 strongly induced Cvsus1 expression, up to the level accumulated in pollinated seeds. These results suggest that Cvsus1 is induced in maternal tissues via signals from the zygotic tissues, and that GA may be one of those signals. PMID:12442898

  8. Seed Size Influence On Germination And Seedling Development Of Cowpea (Vigna unguiculata (L walp

    Directory of Open Access Journals (Sweden)

    Olayemi Ibukun Ehoniyotan

    2013-09-01

    Full Text Available The effect of seed size on the germination and seedling development was carried out on four local varieties of cowpea (Vigna unguiculata (L walp, oloyin,drum, ifebrown and local white. Each of the varieties were sorted into big and small seeds, 100 seeds each of both big and small seeds of the four Varieties were weighed and the average weights were calculated and recorded. Six seeds of each sort were planted in a plastic pot filled with sandy-loamy soil in three replicates. Results of the various parameters studied revealed that germination efficiency was similar in both big and small seeds of drum, oloyin and ifebrown but not in small seeds of local white, weights of 100 seeds from big seeds were much heavier than those of small seeds. Number of leaves, leaf length, leaf breadth, leaf area, stem height and dry weight, were consistently higher in cowpea plants from big seeds than the small seeds of all the varieties. Number of seeds per pod was higher in cowpea from big seeds in all the varieties except in drum where number of seeds was higher in plants from small seeds. In general, seedlings emerging from big seeds of all the cowpea varieties performed better in all the parameters studied than those emerging from smaller seeds.

  9. Exploration Space Suit Architecture: Destination Environmental-Based Technology Development

    Science.gov (United States)

    Hill, Terry R.

    2010-01-01

    This paper picks up where EVA Space Suit Architecture: Low Earth Orbit Vs. Moon Vs. Mars (Hill, Johnson, IEEEAC paper #1209) left off in the development of a space suit architecture that is modular in design and interfaces and could be reconfigured to meet the mission or during any given mission depending on the tasks or destination. This paper will walk though the continued development of a space suit system architecture, and how it should evolve to meeting the future exploration EVA needs of the United States space program. In looking forward to future US space exploration and determining how the work performed to date in the CxP and how this would map to a future space suit architecture with maximum re-use of technology and functionality, a series of thought exercises and analysis have provided a strong indication that the CxP space suit architecture is well postured to provide a viable solution for future exploration missions. Through the destination environmental analysis that is presented in this paper, the modular architecture approach provides the lowest mass, lowest mission cost for the protection of the crew given any human mission outside of low Earth orbit. Some of the studies presented here provide a look and validation of the non-environmental design drivers that will become every-increasingly important the further away from Earth humans venture and the longer they are away. Additionally, the analysis demonstrates a logical clustering of design environments that allows a very focused approach to technology prioritization, development and design that will maximize the return on investment independent of any particular program and provide architecture and design solutions for space suit systems in time or ahead of being required for any particular manned flight program in the future. The new approach to space suit design and interface definition the discussion will show how the architecture is very adaptable to programmatic and funding changes with

  10. Landscape Architecture in Contemporary Danish Suburban Development

    DEFF Research Database (Denmark)

    Roden, Tina Maria

    2013-01-01

    projects of suburban residential development in Hillerød (Ullerødbyen) and Odense (Bellinge Fælled) by introducing yet unpublished interview material from conversations with the respective architects behind the projects: Stig L. Andersson (SLA) and Nina Jensen (Schønherr a/s). Facing today’s effort to...

  11. Architectures for Developing Multiuser, Immersive Learning Scenarios

    Science.gov (United States)

    Nadolski, Rob J.; Hummel, Hans G. K.; Slootmaker, Aad; van der Vegt, Wim

    2012-01-01

    Multiuser immersive learning scenarios hold strong potential for lifelong learning as they can support the acquisition of higher order skills in an effective, efficient, and attractive way. Existing virtual worlds, game development platforms, and game engines only partly cater for the proliferation of such learning scenarios as they are often…

  12. Developer Stories: Improving Architecture in Agile Practice

    DEFF Research Database (Denmark)

    Tjørnehøj, Gitte; Jensen, Rolf Njor; Platz, Niels

    2007-01-01

    Within the field of Software Engineering emergence of agile methods has been a hot topic since the late 90s. eXtreme Programming (XP) ([1]) was one of the first agile methods and is one of the most well-known. However research has pointed to weaknesses in XP regarding supporting development of...

  13. Physiology of tuber development and stolon architecture

    NARCIS (Netherlands)

    Roumeliotis, E.

    2012-01-01

    In this thesis, we used phytohormonal applications, cloning of potato genes related to phytohormone biosynthesis and investigated the  content of several phytohormones in order to clarify how different phytohormones work together to achieve potato tuber development. Our research revealed that p

  14. Impact of Seed Transmission on the Infection and Development of Pitch Canker Disease in Pinus radiata

    OpenAIRE

    Margarita Evira-Recuenco; Eugenia Iturritxa; Rosa Raposo

    2015-01-01

    The aim of this study was to determine the impact of seed inoculum on subsequent disease development in nurseries, specifically studying incidence of seed infection, transmission rate of seed to seedlings, and rate of disease development from the primary inoculum source. Transmission rate of Fusarium circinatum (F. circinatum) from symptomatic trees of Pinus radiata (P. radiata) to seed was 0.73%, being the fungus mainly on the coat. Seed infection incidence was positively correlated with tre...

  15. Sex differences in the genetic architecture of lifespan in a seed beetle: extreme inbreeding extends male lifespan

    DEFF Research Database (Denmark)

    Bilde, T.; Maklakov, Alexei A.; Meisner, Katrine;

    2009-01-01

    Background Sex differences in lifespan are ubiquitous throughout the animal kingdom but the causes underlying this phenomenon remain poorly understood. Several explanations based on asymmetrical inheritance patterns (sex chromosomes or mitochondrial DNA) have been proposed, but these ideas have...... rarely been tested experimentally. Alternatively, sexual dimorphism in lifespan could result from sex-specific selection, caused by fundamental differences in how males and females optimize their fitness by allocating resources into current and future reproduction. Results Here we used sex......-specific responses to inbreeding to study the genetic architecture of lifespan and mortality rates in Callosobruchus maculatus, a seed beetle that shows sexual dimorphism in lifespan. Two independent assays revealed opposing sex-specific responses to inbreeding. The combined data set showed that inbred males live...

  16. A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice.

    Science.gov (United States)

    Wang, Xin; Zhou, Wei; Lu, Zhanhua; Ouyang, Yidan; O, Chol Su; Yao, Jialing

    2015-10-01

    Storage lipid is a vital component for maintaining structure of seed storage substances and valuable for rice quality and food texture. However, the knowledge of lipid transporting related genes and their function in seed development have not been well elucidated yet. In this study, we identified OsLTPL36, a homolog of putative lipid transport protein, and showed specific expression in rice developing seed. Transcriptional profiling and in situ hybridization analysis confirmed that OsLTPL36 was exclusively expressed in developing seed coat and endosperm aleurone cells. Down-regulated expression of OsLTPL36 led to decreased seed setting rate and 1000-grain weight in transgenic plants. Further studies showed that suppressed expression of OsLTPL36 caused chalky endosperm and resulted in reduced fat acid content in RNAi lines as compared with wild type (WT). Histological analysis showed that the embryo development was delayed after down regulation of OsLTPL36. Moreover, impeded seed germination and puny seedling were also observed in the OsLTPL36 RNAi lines. The data demonstrated that OsLTPL36, a lipid transporter, was critical important not only for seed quality but also for seed development and germination in rice. PMID:26398804

  17. Can enterprise architectures reduce failure in development projects?

    NARCIS (Netherlands)

    Janssen, M.F.W.H.A.; Klievink, B.

    2012-01-01

    Purpose: Scant attention has been given to the role of enterprise architecture (EA) in relationship to risk management in information system development projects. Even less attention has been given to the inter-organizational setting. The aim of this paper is to better understand this relationship.

  18. Robotic control architecture development for automated nuclear material handling systems

    International Nuclear Information System (INIS)

    Lawrence Livermore National Laboratory (LLNL) is engaged in developing automated systems for handling materials for mixed waste treatment, nuclear pyrochemical processing, and weapon components disassembly. In support of these application areas there is an extensive robotic development program. This paper will describe the portion of this effort at LLNL devoted to control system architecture development, and review two applications currently being implemented which incorporate these technologies

  19. The New Development Bank in Global Finance and Economic Architecture

    Directory of Open Access Journals (Sweden)

    Alexandra Morozkina

    2015-05-01

    Full Text Available The article addresses the question whether the New Development Bank (NDB will promote the role of the BRICS countriesin the global financial architecture and foster their development. It begins by comparing the key multilateral developmentbanks (World Bank, Asian Development Bank, African Development Bank and Inter-American Development Bank,European Bank for Reconstruction and Development and national development banks of the BRICS countries with thenewly established institution. The NDB’s purpose is to mobilize resources for infrastructure and sustainable developmentprojects in the member countries. This purpose, as author concludes on the base of the analysis, partly duplicates the workof the existing institutions. However, the NDB could add to the functions of the existing institutions and become a significantdevelopment bank for its members. The best way to achieve this significance is to implement multilateral projects in the areasmentioned above. The article also examines the current role of the BRICS countries in the global financial architecture andthe potential for an increased role, brought by the establishment of the BRICS bank. The founding countries of the NDB willjointly determine the volume and directions of its financial aid. In addition, in contrast to the Bretton Woods institutions,the BRICS countries can change the rules of the development financial aid, particularly the conditions and system formonitoring results. The article concludes that the BRICS countries have created a possible way to change the current systemof development finance and therefore to increase the role of the BRICS countries in the global financial architecture.

  20. High toughness alumina ceramics with elongated grains developed from seeds

    Institute of Scientific and Technical Information of China (English)

    XIE; Zhipeng; (谢志鹏); GAO; Lichun; (高立春); LI; Wenchao; (李文超); XU; Lihua; (徐利华); WANG; Xidong; (王习东)

    2003-01-01

    In the present paper, the influence of α-Al2O3 seeds and sintering methods on elongated grain growth and fracture toughness is investigated. The preparation of alumina ceramics started with commercial aluminum hydroxide. Abrasives were introduced to the starting materials by wet-grinding of high-purity alumina milling balls. Abrasives, playing the role of seeds, lowered the transformation temperature of aluminum hydroxide to alumina. Microstructures with elongated grains were developed by hot-pressing for the above calcined powders containing α-Al2O3 seeds, and alumina grain shapes changed with the amount of seeds introduced. However, only equiaxed grains were observed for the samples pressureless sintered. Fracture toughness of the alumina ceramics was dramatically improved by elongated grains. For the sample hot-pressed at 1600℃ for 2 h under 40 MPa pressure, fracture toughness reached 7.1 MPa·m1/2, which is much higher than that of normal alumina ceramics without elongated grains. In addition, high flexural strength of 630 MPa for the hot-pressed samples was also obtained.

  1. Design and development of desiccant seed dryer with airflow inversion and recirculation

    OpenAIRE

    Gill, R. S.; Singh, Sukhmeet; Singh, Parm Pal

    2012-01-01

    A desiccant seed dryer has been developed to dry seed in deep bed at safe temperatures for good shelf life and germination. The dryer consists of two chambers viz., air conditioning control unit and seed drying chamber. It operates in seed drying mode and desiccant regeneration mode. It has provision for recirculation of the drying air to minimise the moisture removal from drying air. Also, it has provision of airflow inversion through deep seed bed for uniform drying. Moisture removal from d...

  2. Rethinking Enterprise Architecture for Sustainable Energy System Development

    Institute of Scientific and Technical Information of China (English)

    Jovita Vasauskaite; Asif Qumer Gill

    2015-01-01

    Abstract-The development of a sustainable energy system throughout an enterprise is a complex task, which requires an agile holistic approach. Such an approach needs to include a variety of objectives including energy strategy formation and strategic decision-making, which are directly related to the analysis and management of the main areas of sustainable development: The economic, technological, environmental, and social. These multidimensional requirements of sustainability are often difficult to achieve within the enterprise, because these aspects are interrelated and influenced by various internal and external environment factors. This paper first reviews the main challenges for an energy system, and then demonstrates how a strategic agile enterprise architecture driven approach could effectively guide the sustainable energy system development. The study presented in this paper provides a holistic approach that contributes to the advancement and usage of literature dealing with issues of sustainable energy system development and agile enterprise architecture, which has not been discussed before to any great extent.

  3. The role of sugars and sugar metabolism genes (sucrose synthase) in arabidopsis thaliana seed development

    OpenAIRE

    Odunlami, Benjamin Oladipo

    2009-01-01

    Seed development in Arabidopsis thaliana, has been studied at several levels. However, little has been done to study the role of sugar metabolism genes in seed pod development in this species. As the fertilized egg progresses to a mature seed, the sugars composition during different stages of the developing changes. These changes are related to metabolic processes in the developing seeds, but also to the activity of sucrose- converting and transporting genes, active at the interphase between ...

  4. The genetic architecture of fitness in a seed beetle: assessing the potential for indirect genetic benefits of female choice

    Directory of Open Access Journals (Sweden)

    Maklakov AA

    2008-10-01

    Full Text Available Abstract Background Quantifying the amount of standing genetic variation in fitness represents an empirical challenge. Unfortunately, the shortage of detailed studies of the genetic architecture of fitness has hampered progress in several domains of evolutionary biology. One such area is the study of sexual selection. In particular, the evolution of adaptive female choice by indirect genetic benefits relies on the presence of genetic variation for fitness. Female choice by genetic benefits fall broadly into good genes (additive models and compatibility (non-additive models where the strength of selection is dictated by the genetic architecture of fitness. To characterize the genetic architecture of fitness, we employed a quantitative genetic design (the diallel cross in a population of the seed beetle Callosobruchus maculatus, which is known to exhibit post-copulatory female choice. From reciprocal crosses of inbred lines, we assayed egg production, egg-to-adult survival, and lifetime offspring production of the outbred F1 daughters (F1 productivity. Results We used the bio model to estimate six components of genetic and environmental variance in fitness. We found sizeable additive and non-additive genetic variance in F1 productivity, but lower genetic variance in egg-to-adult survival, which was strongly influenced by maternal and paternal effects. Conclusion Our results show that, in order to gain a relevant understanding of the genetic architecture of fitness, measures of offspring fitness should be inclusive and should include quantifications of offspring reproductive success. We note that our estimate of additive genetic variance in F1 productivity (CVA = 14% is sufficient to generate indirect selection on female choice. However, our results also show that the major determinant of offspring fitness is the genetic interaction between parental genomes, as indicated by large amounts of non-additive genetic variance (dominance and/or epistasis

  5. Systems Engineering Education Development(SEED)Case Study

    Science.gov (United States)

    Bagg, Thomas C., III; Brumfield, Mark D.; Jamison, Donald E.; Granata, Raymond L.; Casey, Carolyn A.

    2003-01-01

    The Systems Engineering Development Program (SEED) was initiated to help Goddard resolve a Systems Engineering skill shortage. The chronology of events and the experiences of the pilot program are outlined to describe the development of the present program. The program goals are included in order to give a focus on what the developers saw as the program drivers. Lessons learned from a pilot program were incorporated into the present program. This program is constantly learning from its past efforts and looks for continuous improvement. We list several future ideas for improvement and change.

  6. Development of UAS Design Based on Wideband Antenna Architecture

    OpenAIRE

    Franklin Drummond; Gregory Huff

    2015-01-01

    An Unmanned Aerial System (UAS) has been developed which is based on an aerodynamically functionalized planar wideband antenna. The antenna utilizes a planar circular dipole metallization scheme. The aerodynamic structure implements a planform similar to the Nutball flier, a hobbyist flight architecture. The resulting codesign achieved a large impedance bandwidth defined by a voltage standing wave ratio (VSWR) less than 2 from 100 MHz to over 2 GHz and omnidirectional dipole-like radiation pa...

  7. Dynamic software architecture development: Towards an automated process

    OpenAIRE

    Ter Beek M.H.; Bucchiarone A.; Gnesi S.

    2009-01-01

    We propose a software engineering process to aid the development of Dynamic Software Architectures (DSAs). This process is based on the sequential application of a number of formal methods and tools, and it can support software architects throughout the design, analysis and code generation of software systems. To illustrate the process, we apply it to an industrial case study from the Service-Oriented Computing (SOC) domain.

  8. Novel development of heat treatment techniques for seed surface sterilisation

    OpenAIRE

    Borgen, Anders; Krebs, Niels; Langkjær, carsten

    2005-01-01

    Heat treatment to prevent seed borne diseases, e.g. in the form of hot water or warm humid air, will normally heat up the entire seed. Heat treatment of the seed embryo will always have a negative side-effect on seed vigour and the duration of several minutes are difficult to implement in seed plans treating huge volumes of seed. However, in the case of common bunt in wheat and similar diseases, where the inoculum is transmitted as fungal spores on the surface of the seed, an uniform and effi...

  9. UTILIZATION OF POPPY SEEDS (PAPAVER-SOMNIFERUM AND SESAME SEEDS (SESAMUM-INDICUM FOR DEVELOPMENT OF NUTRIA-ENRICHED SNACKS

    Directory of Open Access Journals (Sweden)

    Paul Virginia

    2015-01-01

    Full Text Available Poppy seeds and sesame seeds are good source of carbohydrate, calcium, and energy. Poppy seeds and sesame seeds is rich in vitamin C, Vitamins B1, B2, E, Fiber and calcium. It reduces the risk of cataract, heart disease and cancer.  Poppy seeds are especially rich in Oleic and Linoleic acids. Oleic acids monosaturated fatty acids help lower LDL or “bad cholesterol” and increases HDL or “good cholesterol” levels in the blood. Sesame seeds contain a high quality protein,  rich in soluble fiber. The combination of the oil and the fiber makes sesame seeds ideal laxative. The present research was conducted with the objective to develop nutrient dense products from a combination of poppy seeds and sesame seeds to assess their sensory properties and to determine the nutritive value of the prepared products. Organoleptic evaluation was carried out using nine point hedonic scales. Nutritive value was calculated by using food composition tables given by ICMR. On the basic of findings, it was observed that in case of Idli, Biscuits and Chutney the treatment T2 (20% incorporation of poppy seeds and sesame seeds found best in all sensory parameters colour, texture, taste and overall acceptability. For halwa and Dhokla the treatment T1 (15% incorporation of poppy seeds and sesame seeds found best in all sensory parameters. Halwa was rich in energy and calcium content (447.4kcal/100kcal and 477.5mg/100g respectively. Idli was highest in energy (340kcal/100kcal, calcium, 637.2mg/100g.Dhokla was rich in energy and calcium content (333.6kcal/100kcal, 580.1g/100g and Chutney was rich in energy and calcium content (433.6kcal/100kcal and 611.3mg/100g respectively. Biscuit was rich in energy and calcium content (456.3kcal/100kcal and 616.8/100g.The cost of the products ranged between Rs 18.5 for Halwa, Rs 20.68 for Idli, Rs. 19.49 for Dhokla, Rs. 22.04 for Chutney, Rs. 20.84 for Biscuit per 100g of dry ingredients. 

  10. Development of nutritious snacks by incorporation of amaranth seeds, watermelon seeds and their flour

    OpenAIRE

    Paul Virginia; Ruchi; Paul Ajit

    2014-01-01

    The present study was carried out with the objectives to find out the sensory acceptability, the nutrient content and cost of prepared products. The products prepared were “Biscuits”, “Mathri” and “Laddoo” by incorporation of amaranth seeds, watermelon seeds and their flour in different proportions (10:10, 20:10, and 30:10) served as treatments T1, T2 and T3 respectively T0, without incorporation of amaranth seeds, watermelon seeds and their flour served as control. The products were organole...

  11. SpaceWire model development technology for satellite architecture.

    Energy Technology Data Exchange (ETDEWEB)

    Eldridge, John M.; Leemaster, Jacob Edward; Van Leeuwen, Brian P.

    2011-09-01

    Packet switched data communications networks that use distributed processing architectures have the potential to simplify the design and development of new, increasingly more sophisticated satellite payloads. In addition, the use of reconfigurable logic may reduce the amount of redundant hardware required in space-based applications without sacrificing reliability. These concepts were studied using software modeling and simulation, and the results are presented in this report. Models of the commercially available, packet switched data interconnect SpaceWire protocol were developed and used to create network simulations of data networks containing reconfigurable logic with traffic flows for timing system distribution.

  12. An Architecture to Develop Multimodal Educative Applications with Chatbots

    Directory of Open Access Journals (Sweden)

    David Griol

    2013-03-01

    Full Text Available Animated characters are beginning to be used as pedagogical tools, as they have the power to capture students’ attention and foster their motivation for discovery and learning. However, in order for them to be widely employed and accepted as a learning resource, they must be easy to use and friendly. In this paper we present an architecture that facilitates building interactive pedagogical chatbots that can interact with students in natural language. Our proposal provides a modular and scalable framework to develop such systems efficiently. Additionally, we present Geranium, a system that helps children to appreciate and protect their environment with an interactive chatbot developed following our scheme.

  13. A development of digital plant protection system architecture

    International Nuclear Information System (INIS)

    The digital plant protection system (DPPS) which have a large number of advantages compared to current analog protection system has been developed in various field. The major disadvantages of digital system are, however, vulnerable to faults of processor and software. To overcome the disadvantages, the concept of segment and partition in a channel has been developed. Each segment in a channel is divided from sensor to reactor trip and engineered safety features, which is based on the functional diversity of input signals against the various plant transient phenomena. Each partition allocates the function module to an independent processing module in order to process and isolate the faults of each module of a segment. A communication system based on the deterministic protocol with the predictable and hard real-time characteristics has been developed in order to link the various modules within a segment. The self-diagnostics including online test and periodic test procedures are developed in order to increase the safety, reliability and availability of DPPS. The developed DPPS uses the off-the-shelf DSP (digital signal processor) and adopts VME bus architecture, which have sufficient operation experience in the industry. The verification and validation and quality assurance of software has been developed and the architecture and protocol of deterministic communication system has been researched

  14. Development of an unmanned maritime system reference architecture

    Science.gov (United States)

    Duarte, Christiane N.; Cramer, Megan A.; Stack, Jason R.

    2014-06-01

    The concept of operations (CONOPS) for unmanned maritime systems (UMS) continues to envision systems that are multi-mission, re-configurable and capable of acceptable performance over a wide range of environmental and contextual variability. Key enablers for these concepts of operation are an autonomy module which can execute different mission directives and a mission payload consisting of re-configurable sensor or effector suites. This level of modularity in mission payloads enables affordability, flexibility (i.e., more capability with future platforms) and scalability (i.e., force multiplication). The modularity in autonomy facilitates rapid technology integration, prototyping, testing and leveraging of state-of-the-art advances in autonomy research. Capability drivers imply a requirement to maintain an open architecture design for both research and acquisition programs. As the maritime platforms become more stable in their design (e.g. unmanned surface vehicles, unmanned underwater vehicles) future developments are able to focus on more capable sensors and more robust autonomy algorithms. To respond to Fleet needs, given an evolving threat, programs will want to interchange the latest sensor or a new and improved algorithm in a cost effective and efficient manner. In order to make this possible, the programs need a reference architecture that will define for technology providers where their piece fits and how to successfully integrate. With these concerns in mind, the US Navy established the Unmanned Maritime Systems Reference Architecture (UMS-RA) Working Group in August 2011. This group consists of Department of Defense and industry participants working the problem of defining reference architecture for autonomous operations of maritime systems. This paper summarizes its efforts to date.

  15. Landscape architecture as an entity of property development in Wilayah Persekutuan, Putrajaya

    OpenAIRE

    Ahmad Nasyution bin Abdul Razak; Anuar bin Alias

    2015-01-01

    This study explores the relationship between landscape architecture and property development. Landscape architecture emphasizes the nature of conservation, development of public parks, city parks, local parks, artificial forests, lakes, recreational areas, outdoor recreation centers, open space systems and other components of landscape architecture in providing opportunities and benefits for real estate development. Backed by ample literature review, this study reveals the significant relatio...

  16. Effects of Seed Injury and Injection with Gibberellic Acid and Paclobutrazol on Fruit Drop and Seed Development in “Beni Shimizu” Peach

    OpenAIRE

    福田, 文夫; 近阪, 昌之; 久保田, 尚浩

    2006-01-01

    The relationsships between fruit drop induced by seed injury and seed development were investigated in “Beni Shimizu” peach. Seed injury(Injury) was done by drilling from opposite side of suture line upto seed coat using an electric drill(outre diameter of 2.5mm) at 40, 50, 60, 72 and 80 days after full bloom(DAFB). Injections of gibberellic acid(GA) and paclobutrazol(PBZ) (Injection), which relate to fruit drop and seed development were carried out just after the injury for seed. In all of t...

  17. PROJECT ORIENTATION: A PROPOSAL TO DEVELOP A SERVICEORIENTED ARCHITECTURE

    OpenAIRE

    Daniel Valente Serman

    2010-01-01

    A service-oriented architecture (SOA) is occasionally seen as a complex and undefeatable challenge (Serman, 2007). This feeling is motivated by the lack of attention from practitioners and from the academy on the means for its construction. The work aims to bring the portfolio management of IT projects as an option to guide the SOA development. A review of the literature on SOA was carried out, including case studies on the SOA adoption. A case study was also done, which analyzed some project...

  18. Development of irradiation support devices for production of brachytherapy seeds

    Energy Technology Data Exchange (ETDEWEB)

    Mattos, Fabio R.; Rostelato, Maria Elisa C.M.; Zeituni, Carlos A.; Souza, Carla D.; Moura, Joao A.; Peleias Junior, Fernando S.; Karan Junior, Dib; Feher, Anselmo; Oliveira, Tiago B.; Benega, Marcos A.G., E-mail: tiagooliveira298@gmail.com, E-mail: mattos.fr@gmail.com, E-mail: elisaros@ipen.br, E-mail: czeituni@ipen.br, E-mail: carladdsouza@yahoo.com.br, E-mail: jamoura@ipen.br, E-mail: ernandopeleias@gmail.com, E-mail: s, E-mail: dib.karan@usp.br, E-mail: afeher@ipen.br, E-mail: marcosagbenega@gmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    Ophthalmic tumors treatment with brachytherapy sources has been widely used as a primary or secondary therapy for non-malignant or malignant tumors, for example, choroid melanoma, and retinoblastoma. Ruthenium-106, Iodine-125, Palladium -103, Gold-198 and Iridium-192, are some radionuclides that can be applied for treatment of ocular tumors. These sources are in small sizes (a few millimeters) and different shapes (rods, wires, disks). To ensure high accuracy during treatment, they are positioned in eye applicators, specially designed to fit on the surface of tumor. The Nuclear and Energy Research Institute (IPEN/CNEN) in a partnership with Paulista Medicine School (UNIFESP) created a project that aims to develop a prototype of Iridium-192 seeds for treatment of eye cancer. This seed consists in a core of Ir -Pt alloy (20%-80%) with a length of 3 mm, to be activated in IPEN's IEA-R1 Reactor, and a titanium capsule sealing the core. It was imperative to develop a sustainer device for irradiation. This piece is used to avoid overlapping of one cores and, therefore, avoiding the 'shadow effect' that does not allow full activation of each core due to the high density. (author)

  19. Relationships between changes of kernel nutritive components and seed vigor during development stages of F1 seeds of sh 2 sweet corn*

    OpenAIRE

    Cao, Dong-dong; Hu, Jin; Huang, Xin-xian; Wang, Xian-Ju; Guan, Ya-jing; Wang, Zhou-fei

    2008-01-01

    The changes of kernel nutritive components and seed vigor in F1 seeds of sh 2 sweet corn during seed development stage were investigated and the relationships between them were analyzed by time series regression (TSR) analysis. The results show that total soluble sugar and reducing sugar contents gradually declined, while starch and soluble protein contents increased throughout the seed development stages. Germination percentage, energy of germination, germination index and vigor inde...

  20. Development of a computerized handbook of architectural plans: Ontwikkeling van een gecomputeriseerd handboek van architectonische plattegronden

    OpenAIRE

    Koutamanis, A.

    1990-01-01

    The dissertation investigates an approach to the development of visual / spatial computer representations for architectural purposes through the development of the computerized handbook of architectural plans (chap), a knowledge-based computer system capable of recognizing the metric properties of architectural plans. This investigation can be summarized as an introduction of computer vision to the computerization of architectural representations: chap represents an attempt to automate recogn...

  1. Programmed cell death during development of cowpea (Vigna unguiculata (L.) Walp.) seed coat.

    Science.gov (United States)

    Lima, Nathália Bastos; Trindade, Fernanda Gomes; da Cunha, Maura; Oliveira, Antônia Elenir Amâncio; Topping, Jennifer; Lindsey, Keith; Fernandes, Kátia Valevski Sales

    2015-04-01

    The seed coat develops primarily from maternal tissues and comprises multiple cell layers at maturity, providing a metabolically dynamic interface between the developing embryo and the environment during embryogenesis, dormancy and germination of seeds. Seed coat development involves dramatic cellular changes, and the aim of this research was to investigate the role of programmed cell death (PCD) events during the development of seed coats of cowpea [Vigna unguiculata (L.) Walp.]. We demonstrate that cells of the developing cowpea seed coats undergo a programme of autolytic cell death, detected as cellular morphological changes in nuclei, mitochondria, chloroplasts and vacuoles, DNA fragmentation and oligonucleosome accumulation in the cytoplasm, and loss of membrane viability. We show for the first time that classes 6 and 8 caspase-like enzymes are active during seed coat development, and that these activities may be compartmentalized by translocation between vacuoles and cytoplasm during PCD events. PMID:25142352

  2. Relationships between changes of kernel nutritive components and seed vigor during development stages of F1 seeds of sh2 sweet corn

    Institute of Scientific and Technical Information of China (English)

    Dong-dong CAO; Jin HU; Xin-xian HUANG; Xian-ju WANG; Ya-jing GUAN; Zhou-fei WANG

    2008-01-01

    The changes of kernel nutritive components and seed vigor in F1 seeds of sh2 sweet corn during seed development stage were investigated and the relationships between them were analyzed by time series regression (TSR) analysis. The results show that total soluble sugar and reducing sugar contents gradually declined, while starch and soluble protein contents increased throughout the seed development stages. Germination percentage, energy of germination, germination index and vigor index gradually increased along with seed development and reached the highest levels at 38 d aider pollination (DAP). The TSR showed that, during 14 to 42 DAP, total soluble sugar content was independent of the vigor parameters determined in present experiment, while the reducing sugar content had a significant effect on seed vigor. TSR equations between seed reducing sugar and seed vigor were also developed. There were negative correlations between the seed reducing sugar content and the germination percentage, energy of germination, germination index and vigor index, respectively. It is suggested that the seed germination, energy of germination, germination index and vigor index could be predicted by the content of reducing sugar in sweet corn seeds during seed development stages.

  3. Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

    OpenAIRE

    Ayeh Kwadwo; Lee YeonKyeong; Ambrose Mike J; Hvoslef-Eide Anne

    2011-01-01

    Abstract Background The def mutant pea (Pisum sativum L) showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations. Findings Pod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either...

  4. Development and structure of hawthorn seeds (Crataegus L.) from species occurring in Poland

    OpenAIRE

    Stanisław Pelc; Krystyna Ptak

    2014-01-01

    The development, internal and external structure of seeds of Crataegus monogyna Jacq., C. curvisepala Lindm., C. lindmannii Hrabĕt-Uhr., C. oxyacantha L. em. Jacq., C. palmstruchii Lindm., C. macrocarpa Hegetsch were investigated. It was found that the embryo development is specific, and the development of endosperm precedes that of the embryo. In mature seeds of the examined species endosperm is present under the seed coat and the embryo with large cotyledones and radicle is completely immer...

  5. Glutathione Dynamics in Arabidopsis Seed Development and Germination

    OpenAIRE

    Sumugat, Mae Rose S.

    2004-01-01

    Seed desiccation and germination have great potential for oxidative stress. Glutathione, one of the most abundant antioxidants in plant cells, is a crucial to the plant's defense mechanisms. To better understand glutathione's responses during these two stages, we examined its dynamics in wildtype Arabidopsis seeds and in a transgenic line containing an antisense glutathione reductase2 (anGR2) cDNA insert. Seeds from the two genotypes were compared morphologically. Glutathione levels in maturi...

  6. A General Architecture For Developing A Sustainable Elderly Care e-Health System

    OpenAIRE

    Bai, Guohua; Guo, Yang

    2011-01-01

    Based on some identified problems and challenges to the current healthcare system, this paper proposes architecture for developing e-health system to meet the challenges and resolve some problems. The architecture is based on a solid theoretical model of human activity, and it has properties such as sustainability, generality, and expandability. We demonstrate the properties of the architecture through an ongoing research project. We concluded that this architecture can be widely used to inte...

  7. Effect of seed coat on the seed germination and seedling development of Calophyllum brasiliense Cambess. (Clusiaceae

    Directory of Open Access Journals (Sweden)

    Valquíria Aparecida Mendes de Jesus

    2014-10-01

    Full Text Available This work aimed to study the effect of the Calophyllum brasiliense seed coat on the seed germination process. To this end, three experiments were conducted in laboratory, greenhouse and screenhouse. From a total of six treatments, five are related to the seed coat (mechanical scarification; mechanical scarification followed by 2 hours in water, chemical scarification, hot water immersion and complete seed coat removal and one control. Laboratory and greenhouse experiments were conducted in a completely randomized design (CRD. Screenhouse experiment was conducted in a completely randomized block design (RBD. We evaluated the total percentage, the speed index and the average time of germination or emergence. Data were subjected to analysis of variance and means compared by LSD test, at 5%. Under the conditions of this work, it was possible to infer that, in laboratory, mechanical scarification followed by 2 hours in water increases the proportion and germination speed index (GSI, in the greenhouse, the complete seed coat removal increases the percentage and emergence speed index (ESI, and in the screenhouse, mechanical scarification followed by 2 hours in water and chemical scarification presented the best results. The average germination time was not significantly different in the three experiments evaluated.

  8. Genome-wide analysis of coordinated transcript abundance during seed development in different Brassica rapa morphotypes

    OpenAIRE

    Basnet, R.K.; Moreno Pachón, N.M.; Lin, K.; Bucher, J; Visser, R.G.F.; Maliepaard, C.A.; Bonnema, A.B.

    2013-01-01

    Brassica seeds are important as basic units of plant growth and sources of vegetable oil. Seed development is regulated by many dynamic metabolic processes controlled by complex networks of spatially and temporally expressed genes. We conducted a global microarray gene co-expression analysis by measuring transcript abundance of developing seeds from two diverse B. rapa morphotypes: a pak choi (leafy-type) and a yellow sarson (oil-type), and two of their doubled haploid (DH) progenies, (1) to ...

  9. Analysis of Gene Expression Patterns during Seed Coat Development in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    Gillian Dean; George Haughn; YoncgGuo Cao; DaoQuan Xiang; Nicholas J. Provart; Larissa Ramsay; Abdul Ahada; Rick White; Gopalan Selvaraj; Raju Datla

    2011-01-01

    The seed coat is important for embryo protection,seed hydration,and dispersal.Seed coat composition is also of interest to the agricultural sector,since it impacts the nutritional value for humans and livestock alike.Although some seed coat genes have been identified,the developmental pathways controlling seed coat development are not completely elucidated,and a global genetic program associated with seed coat development has not been reported.This study uses a combination of genetic and genomic approaches in Arabidopsis thaliana to begin to address these knowledge gaps.Seed coat development is a complex process whereby the integuments of the ovule differentiate into specialized cell types.In Arabidopsis,the outermost layer of cells secretes mucilage into the apoplast and develops a secondary cell wall known as a columella.The layer beneath the epidermis,the palisade,synthesizes a secondary cell wall on its inner tangential side.The innermost layer (the pigmented layer or endothelium) produces proanthocyanidins that condense into tannins and oxidize,giving a brown color to mature seeds.Genetic separation of these cell layers was achieved using the ap2-7 and tt16-1 mutants,where the epidermis/palisade and the endothelium do not develop respectively.This genetic ablation was exploited to examine the developmental programs of these cell types by isolating and collecting seed coats at key transitions during development and performing global gene expression analysis.The data indicate that the developmental programs of the epidermis and the pigmented layer proceed relatively independently.Global expression datasets that can be used for identification of new gene candidates for seed coat development were generated.These dataset provide a comprehensive expression profile for developing seed coats in Arabidopsis,and should provide a useful resource and reference for other seed systems.

  10. Void space inside the developing seed of Brassica napus and the modelling of its function

    OpenAIRE

    Verboven, Pieter; Herremans, Els; Borisjuk, Ljudmilla; Helfen, Lukas; Ho, Quang Tri; Tschiersch, Henning; Fuchs, Johannes; Nicolai, Bart; Rolletschek, Hardy

    2013-01-01

    The developing seed essentially relies on external oxygen to fuel aerobic respiration, but it is currently unknown how oxygen diffuses into and within the seed, which structural pathways are used and what finally limits gas exchange. By applying synchrotron X-ray computed tomography to developing oilseed rape seeds we uncovered void spaces, and analysed their three-dimensional assembly. Both the testa and the hypocotyl are well endowed with void space, but in the cotyledons, spaces were small...

  11. Development and structure of hawthorn seeds (Crataegus L. from species occurring in Poland

    Directory of Open Access Journals (Sweden)

    Stanisław Pelc

    2014-02-01

    Full Text Available The development, internal and external structure of seeds of Crataegus monogyna Jacq., C. curvisepala Lindm., C. lindmannii Hrabĕt-Uhr., C. oxyacantha L. em. Jacq., C. palmstruchii Lindm., C. macrocarpa Hegetsch were investigated. It was found that the embryo development is specific, and the development of endosperm precedes that of the embryo. In mature seeds of the examined species endosperm is present under the seed coat and the embryo with large cotyledones and radicle is completely immersed in it. The external appearance of the seeds of particular species differs, and on this basis their specific appurtenance may be determined.

  12. Managing Innovation and Business Development Using Enterprise Architecture

    DEFF Research Database (Denmark)

    Tambo, Torben; Bækgaard, Lars

    2011-01-01

    technologies. In management of IT, it has become increasingly popular to use Enterprise Architecture (EA) as a method and supported by a series of formal frameworks. EA maps artifacts and motives against the business strategy. In this paper, MOT and EA are reviewed for their mutual potentials and issues. Two...... well planned technological changes. Management of technology (MOT) addresses identification, selection, (long term) planning, designing, implementation and operation of technology based business development. Information Technology (IT) is a key enabler for a vast range of contemporary corporate...... case studies illustrate how enterprises can make major changes derived from business strategy observing both MOT and EA. This suggests a combined view inspired from the IT business’ dedication to EA using EA’s formalisms and the management orientation of MOT to improve understanding technological...

  13. DEVELOPMENT PROCESS OF BEAUTY AND HOLISM IN IRAN MOSQUE ARCHITECTURE

    OpenAIRE

    SHOJAEE, Farshid; PAEEZE, Mehran

    2015-01-01

    Abstract. The final goal of this article is to explain the accurate meaning of beauty ,finding general criteria of these concepts ,and mixing and their concrete appearance in Islamic architecture ,in particular mosques .Since finding a unique and basic definition for beauty in Islamic art and architecture requires vast research and existing definitions in Western and Islamic  philosophy  ,art, and architecture are significantly different , this research tries to explain these concepts clearly...

  14. Drought stress delays endosperm development and misregulates genes associated with cytoskeleton organization and grain quality proteins in developing wheat seeds.

    Science.gov (United States)

    Begcy, Kevin; Walia, Harkamal

    2015-11-01

    Drought stress is a major yield-limiting factor for wheat. Wheat yields are particularly sensitive to drought stress during reproductive development. Early seed development stage is an important determinant of seed size, one of the yield components. We specifically examined the impact of drought stress imposed during postzygotic early seed development in wheat. We imposed a short-term drought stress on plants with day-old seeds and observed that even a short-duration drought stress significantly reduced the size of developing seeds as well as mature seeds. Drought stress delayed the developmental transition from syncytial to cellularized stage of endosperm. Coincident with reduced seed size and delayed endosperm development, a subset of genes associated with cytoskeleton organization was misregulated in developing seeds under drought-stressed. Several genes linked to hormone pathways were also differentially regulated in response to drought stress in early seeds. Notably, drought stress strongly repressed the expression of wheat storage protein genes such as gliadins, glutenins and avenins as early as 3 days after pollination. Our results provide new insights on how some of the early seed developmental events are impacted by water stress, and the underlying molecular pathways that can possibly impact both grain size and quality in wheat. PMID:26475192

  15. Developing the architecture for the Climate Information Portal for Copernicus

    Science.gov (United States)

    Som de Cerff, Wim; Thijsse, Peter; Plieger, Maarten; Pascoe, Stephen; Jukes, Martin; Leadbetter, Adam; Goosen, Hasse; de Vreede, Ernst

    2015-04-01

    environment and society, but will develop an end to end processing chain (indicator toolkit), from comprehensive information on the climate state through to highly aggregated decision relevant products. This processing chain will be demonstrated within three thematic areas: water, rural and urban. Indicators of climate change and climate change impact will be provided, and a toolkit to update and post process the collection of indicators will be integrated into the portal. For the indicators three levels (Tiers) have been loosely defined: Tier 1: field summarising properties of the climate system; e.g. temperature change; Tier 2: expressed in terms of environmental properties outside the climate system; e.g. flooding change; Tier 3: expressed in social and economic impact. For the architecture, CLIPC has two interlocked themes: 1. Harmonised access to climate datasets derived from models, observations and re-analyses 2. A climate impact toolkit to evaluate, rank and aggregate indicators For development of the CLIPC architecture an Agile 'storyline' approach is taken. The storyline is a real world use case and consists of producing a Tier 3 indicator (Urban Heat Vulnerability) and making it available through the CLIPC infrastructure for a user group. In this way architecture concepts can be directly tested and improved. Also, the produced indicator can be shown to users to refine requirements. Main components of the CLIPC architecture are 1) Data discovery and access, 2) Data processing, 3) Data visualization, 4) Knowledge base and 5) User Management. The Data discovery and access component main challenge is to provide harmonized access to various sources of climate data (ngEO, EMODNET/SeaDataNet, ESGF, MyOcean). The discovery service concept will be provided using a CLIPC data and data product catalogue and via a structured data search on selected infrastructures, using NERC vocabulary services and mappings. Data processing will be provided using OGC WPS services, linking

  16. Landscape architecture as an entity of property development in Wilayah Persekutuan, Putrajaya

    Directory of Open Access Journals (Sweden)

    Ahmad Nasyution bin Abdul Razak

    2015-12-01

    Full Text Available This study explores the relationship between landscape architecture and property development. Landscape architecture emphasizes the nature of conservation, development of public parks, city parks, local parks, artificial forests, lakes, recreational areas, outdoor recreation centers, open space systems and other components of landscape architecture in providing opportunities and benefits for real estate development. Backed by ample literature review, this study reveals the significant relationship between land, real estate and landscape architecture. Landscape architecture which provides an open park and green space can be expanded to benefit the social, environment and economy through optimal used of space in property development project. Hence, this study discusses the objectives which are to determine significant factors in the development of landscape architecture, to identify the importance of landscape architecture in real estate development, and to assess the relationship between landscape architecture and property development land. This study involved 50 respondents from renowned developers; Putrajaya Holdings Sdn Bhd (PJH, Selangor State Development Corporation (PKNS, the local authority; Putrajaya Corporation (PPJ, Kajang Municipal Council (MPKj, relevant agencies; Valuation and Property Services Department-JPPH (Ministry of Finance, Department of Town and Country Planning (Peninsular Malaysia, developers registered with ReHDA and residents / property owners in the study area. To conclude, this study offers adequate benefits to policy makers, also relevant agencies such as the local authorities, landowners, property developers, the relevant professionals and communities on the positive effects and significant relationships between landscape architecture and property development.

  17. Developing A System Architecture For Holonic Shop Floor Control

    DEFF Research Database (Denmark)

    Sørensen, Christian; Langer, Gilad; Alting, Leo

    1998-01-01

    This paper describes theresults of research regarding the emerging theory of Holonic Manufacturing Systems. This theory and in particular itscorresponding reference architecture serves as the basis for thedevelopment of a system-architecture for shop floor controlsystems in a multi-cellular conte...

  18. Research on the Software Development Architecture of Naval Simulation Training Standard Console

    OpenAIRE

    Xu Jing; Xu Ming; Li Tie

    2013-01-01

    A component based Architecture is proposed to meet the requirements of the software development architecture of naval simulation training standard console. The Standard Element Database (SDD) and management system, the Foundation Classes Library (FCL), the Standard Object Model Development Tool(S-OMDT) are developed which make developing simpler and rapid

  19. Demand Activated Manufacturing Architecture (DAMA) supply chain collaboration development methodology

    Energy Technology Data Exchange (ETDEWEB)

    PETERSEN,MARJORIE B.; CHAPMAN,LEON D.

    2000-03-15

    The Demand Activated Manufacturing Architecture (DAMA) project during the last five years of work with the U.S. Integrated Textile Complex (retail, apparel, textile, and fiber sectors) has developed an inter-enterprise supply chain collaboration development methodology. The goal of this methodology is to enable a supply chain to work more efficiently and competitively. The outcomes of this methodology include: (1) A definitive description and evaluation of the role of business cultures and supporting business organizational structures in either inhibiting or fostering change to a more competitive supply chain; (2) ``As-Is'' and proposed ``To-Be'' supply chain business process models focusing on information flows and decision-making; and (3) Software tools that enable and support a transition to a more competitive supply chain, which results form a business driven rather than technologically driven approach to software design. This methodology development will continue in FY00 as DAMA engages companies in the soft goods industry in supply chain research and implementation of supply chain collaboration.

  20. Development of a Computerized Measurement System for in-Row Seed Spacing Accuracy

    OpenAIRE

    ÖNAL, Okan; ÖNAL, İsmet

    2009-01-01

    The aim of this study was to develop a computerized measurement system (CMS) combined with a sticky belt test stand for examining in-row seed spacing distribution accuracy. For this purpose 8 performance parameters were selected for precision seeding: mean seed spacing, the standard deviation, the multiples index, the miss index, the quality of feed index, the precision, the population index, and the coefficient of precision (CP-3). The CMS hardware consisted of a high precision optical mouse...

  1. Information extraction from articles for the elaboration of the regulatory networks involved in Arabidopsis seed development

    OpenAIRE

    Dubreucq, Bertrand; Valsamou, Dialekti; Fatihi, Abdelhak; Chaix, Estelle; Bossy, Robert; Bessieres, Philippe; Deleger, Louise; Zweigenbaum, Pierre; Nédellec, Claire; Lepiniec, Loic

    2015-01-01

    Seed is the main vector for breeding and production of annual field crops, and the accumulation of seed storage compounds (sugars, lipids, proteins) is of primary importance for food, feed and industrial uses. Seed development requires the coordinated growth of different tissues and involves complex genetics and environmental regulations. A comprehensive understanding of the molecular network underlying these regulations remains a major scientific challenge with important potential impact for...

  2. Adaptation of pancreatic islet cyto-architecture during development

    Science.gov (United States)

    Striegel, Deborah A.; Hara, Manami; Periwal, Vipul

    2016-04-01

    Plasma glucose in mammals is regulated by hormones secreted by the islets of Langerhans embedded in the exocrine pancreas. Islets consist of endocrine cells, primarily α, β, and δ cells, which secrete glucagon, insulin, and somatostatin, respectively. β cells form irregular locally connected clusters within islets that act in concert to secrete insulin upon glucose stimulation. Varying demands and available nutrients during development produce changes in the local connectivity of β cells in an islet. We showed in earlier work that graph theory provides a framework for the quantification of the seemingly stochastic cyto-architecture of β cells in an islet. To quantify the dynamics of endocrine connectivity during development requires a framework for characterizing changes in the probability distribution on the space of possible graphs, essentially a Fokker-Planck formalism on graphs. With large-scale imaging data for hundreds of thousands of islets containing millions of cells from human specimens, we show that this dynamics can be determined quantitatively. Requiring that rearrangement and cell addition processes match the observed dynamic developmental changes in quantitative topological graph characteristics strongly constrained possible processes. Our results suggest that there is a transient shift in preferred connectivity for β cells between 1–35 weeks and 12–24 months.

  3. Adaptation of pancreatic islet cyto-architecture during development.

    Science.gov (United States)

    Striegel, Deborah A; Hara, Manami; Periwal, Vipul

    2016-01-01

    Plasma glucose in mammals is regulated by hormones secreted by the islets of Langerhans embedded in the exocrine pancreas. Islets consist of endocrine cells, primarily α, β, and δ cells, which secrete glucagon, insulin, and somatostatin, respectively. β cells form irregular locally connected clusters within islets that act in concert to secrete insulin upon glucose stimulation. Varying demands and available nutrients during development produce changes in the local connectivity of β cells in an islet. We showed in earlier work that graph theory provides a framework for the quantification of the seemingly stochastic cyto-architecture of β cells in an islet. To quantify the dynamics of endocrine connectivity during development requires a framework for characterizing changes in the probability distribution on the space of possible graphs, essentially a Fokker-Planck formalism on graphs. With large-scale imaging data for hundreds of thousands of islets containing millions of cells from human specimens, we show that this dynamics can be determined quantitatively. Requiring that rearrangement and cell addition processes match the observed dynamic developmental changes in quantitative topological graph characteristics strongly constrained possible processes. Our results suggest that there is a transient shift in preferred connectivity for β cells between 1-35 weeks and 12-24 months. PMID:27063927

  4. Developing a scalable modeling architecture for studying survivability technologies

    Science.gov (United States)

    Mohammad, Syed; Bounker, Paul; Mason, James; Brister, Jason; Shady, Dan; Tucker, David

    2006-05-01

    To facilitate interoperability of models in a scalable environment, and provide a relevant virtual environment in which Survivability technologies can be evaluated, the US Army Research Development and Engineering Command (RDECOM) Modeling Architecture for Technology Research and Experimentation (MATREX) Science and Technology Objective (STO) program has initiated the Survivability Thread which will seek to address some of the many technical and programmatic challenges associated with the effort. In coordination with different Thread customers, such as the Survivability branches of various Army labs, a collaborative group has been formed to define the requirements for the simulation environment that would in turn provide them a value-added tool for assessing models and gauge system-level performance relevant to Future Combat Systems (FCS) and the Survivability requirements of other burgeoning programs. An initial set of customer requirements has been generated in coordination with the RDECOM Survivability IPT lead, through the Survivability Technology Area at RDECOM Tank-automotive Research Development and Engineering Center (TARDEC, Warren, MI). The results of this project are aimed at a culminating experiment and demonstration scheduled for September, 2006, which will include a multitude of components from within RDECOM and provide the framework for future experiments to support Survivability research. This paper details the components with which the MATREX Survivability Thread was created and executed, and provides insight into the capabilities currently demanded by the Survivability faculty within RDECOM.

  5. Architecture and Design in eXtreme Programming; Introducing "Developer Stories"

    DEFF Research Database (Denmark)

    Jensen, Rolf Njor; Møller, Thomas; Sönder, Peter;

    2006-01-01

    This article introduces a new practice to eXtreme Programming (XP): Developer stories. The goal of these stories and their creation process is to incorporate architectural planning to XP thus ensuring a viable architecture. By conducting a small development project using XP, we find that establis......This article introduces a new practice to eXtreme Programming (XP): Developer stories. The goal of these stories and their creation process is to incorporate architectural planning to XP thus ensuring a viable architecture. By conducting a small development project using XP, we find that...... establishing a viable architecture might be problematic. Arguing that architectural quality criteria in XP are traceable to traditional criteria, and by pointing to related work regarding incremental continous design, requirements management and large-scale refactorings, we find support for this claim. We...

  6. Ethylene involvement in silique and seed development of canola, Brassica napus L.

    Science.gov (United States)

    Walton, Linda J; Kurepin, Leonid V; Yeung, Edward C; Shah, Saleh; Emery, R J Neil; Reid, David M; Pharis, Richard P

    2012-09-01

    A wide range of plant hormones, including gibberellins (GAs) and auxins are known to be involved in regulating seed and fruit growth and development. Changes in ethylene biosynthesis are also associated with seed and fruit development, but ethylene's role in these processes is poorly understood, as is its possible interaction with the other plant hormones. A major complication of investigating ethylene-induced regulation of developmental processes is ethylene's biphasic mode of action. To investigate ethylene's actions and interactions we used a 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase transgenic canola line. This line evolves significantly less ethylene from its siliques and seeds, relative to plants from a wild type (WT) background. Plants of the transgenic line also had smaller siliques which were associated with reductions in both seed size and seed number. Application of ethephon, a compound that produces ethylene, to plants of the transgenic line restored the WT phenotype for both siliques and seeds. Application of the same dose of ethephon to WT plants diminished both silique and seed development, showing ethylene's biphasic effect and effectively producing the ACC deaminase transgenic phenotype. There were significant decreases in endogenous concentrations of GA(1) and GA(4) and also of indole-3-acetic acid (IAA), between WT seeds and seedless siliques and seeds and siliques from the transgenic line plants. These differences were emphasized during early stages (10-20 days after pollination) of seed and silique development. The above results strongly suggest that ethylene interacts with other endogenous plant hormones in regulating silique and seed development and growth in WT lines of canola. PMID:22809685

  7. Impact of Seed Transmission on the Infection and Development of Pitch Canker Disease in Pinus radiata

    Directory of Open Access Journals (Sweden)

    Margarita Evira-Recuenco

    2015-09-01

    Full Text Available The aim of this study was to determine the impact of seed inoculum on subsequent disease development in nurseries, specifically studying incidence of seed infection, transmission rate of seed to seedlings, and rate of disease development from the primary inoculum source. Transmission rate of Fusarium circinatum (F. circinatum from symptomatic trees of Pinus radiata (P. radiata to seed was 0.73%, being the fungus mainly on the coat. Seed infection incidence was positively correlated with tree disease severity. Seeds also become contaminated with F. circinatum during storage, where high relative humidity had a great effect regardless of temperature. Transmission rates from seeds to symptomatic seedlings measured at 48 days after seed inoculation with 104 and 106 was 28% and 80%, respectively. Seedlings showed symptoms of wilting and dieback, and eventually died within the total duration of this study (475 days. By this time, there were two remaining symptomless seedlings (1% of the total. F. circinatum was recovered from each of them, demonstrating its persistence in seedlings. Fungus populations in roots were estimated between 105 and 107 cfu/g in symptomatic plants for both inoculum doses and up to 104 cfu/g in asymptomatic plants. Disease infection rate was similar in post-emergence damping-off (up two months growing and for established seedlings (up to eight months after seeds were inoculated with 104 spores/mL.

  8. Kin recognition within a seed and the effect of genetic relatedness of an endosperm to its compatriot embryo on maize seed development

    OpenAIRE

    Wu, Chi-Chih; Diggle, Pamela K.; Friedman, William E.

    2013-01-01

    As one of two sexual products resulting from double fertilization in angiosperms, the endosperm nourishes its compatriot embryo during seed development and/or germination and ultimately dies. Theoretical studies suggest that the genetic relatedness of an endosperm to its embryo in the same seed might determine the amount of resources ultimately available for the embryo during seed development. We took advantage of the phenomenon of heterofertilization in cultivated maize to empirically test w...

  9. Using RNA-Seq to profile soybean seed development from fertilization to maturity.

    Directory of Open Access Journals (Sweden)

    Sarah I Jones

    Full Text Available To understand gene expression networks leading to functional properties and compositional traits of the soybean seed, we have undertaken a detailed examination of soybean seed development from a few days post-fertilization to the mature seed using Illumina high-throughput transcriptome sequencing (RNA-Seq. RNA was sequenced from seven different stages of seed development, yielding between 12 million and 78 million sequenced transcripts. These have been aligned to the 79,000 gene models predicted from the soybean genome recently sequenced by the Department of Energy Joint Genome Institute. Over one hundred gene models were identified with high expression exclusively in young seed stages, starting at just four days after fertilization. These were annotated as being related to many basic components and processes such as histones and proline-rich proteins. Genes encoding storage proteins such as glycinin and beta-conglycinin had their highest expression levels at the stages of largest fresh weight, confirming previous knowledge that these storage products are being rapidly accumulated before the seed begins the desiccation process. Other gene models showed high expression in the dry, mature seeds, perhaps indicating the preparation of pathways needed later, in the early stages of imbibition. Many highly-expressed gene models at the dry seed stage are, as expected, annotated as hydrophilic proteins associated with low water conditions, such as late embryogenesis abundant (LEA proteins and dehydrins, which help preserve the cellular structures and nutrients within the seed during desiccation. More significantly, the power of RNA-Seq to detect genes expressed at low levels revealed hundreds of transcription factors with notable expression in at least one stage of seed development. Results from a second biological replicate demonstrate high reproducibility of these data revealing a comprehensive view of the transciptome of seed development in the

  10. Developing Distributed System With Service Resource Oriented Architecture

    OpenAIRE

    Riyanarto Sarno; Hermawan Hermawan

    2012-01-01

    Service Oriented Architecture is a design paradigm in software engineering with which a distributed system is built for an enterprise. This paradigm aims at providing the system as a service through a protocol in web service technology, namely Simple Object Access Protocol (SOAP). However, SOA is service level agreements of webservice. For this reason, this reasearch aims at combining SOA with Resource Oriented Architecture in order to expand scalability of services. This combination creates...

  11. A Methodology to Develop Judgment Skills in Sustainable Architectural Education

    OpenAIRE

    Kenny, Paul; Brophy, Vivienne

    2011-01-01

    Students of sustainability, in particular architectural students, are faced with a vast body of published work that forms an important part of their reference library. The idea of the precedent study is, traditionally, central to the education of an architectural student, introducing them to exemplary projects of all types. However many of the buildings published and purporting to be ‘sustainable’ or ‘green’ lack rigorous and impartial review. In the absence of such credible evide...

  12. Development of a modular integrated control architecture for flexible manipulators. Final report

    International Nuclear Information System (INIS)

    In April 1994, ORNL and SPAR completed the joint development of a manipulator controls architecture for flexible structure controls under a CRADA between the two organizations. The CRADA project entailed design and development of a new architecture based upon the Modular Integrated Control Architecture (MICA) previously developed by ORNL. The new architecture, dubbed MICA-II, uses an object-oriented coding philosophy to provide a highly modular and expandable architecture for robotic manipulator control. This architecture can be readily ported to control of many different manipulator systems. The controller also provides a user friendly graphical operator interface and display of many forms of data including system diagnostics. The capabilities of MICA-II were demonstrated during oscillation damping experiments using the Flexible Beam Experimental Test Bed at Hanford

  13. A Proteomics-Based Platform for Systems Biology Analysis of Soybean Seed Development

    Science.gov (United States)

    A system based on fresh weight and color was used to define eight stages of soybean (Glycine max (L.) Merrill, cv. Jack) seed development. Storage protein, oil, and starch were quantified from each stage, and used along with the morphological characteristics to establish a first-stage model of seed...

  14. Mineral accumulation in vegetative and reproductive tissues during seed development in Medicago truncatula

    Directory of Open Access Journals (Sweden)

    Christina B. Garcia

    2015-08-01

    Full Text Available Enhancing nutrient density in legume seeds is one of several strategies being explored to improve the nutritional quality of the food supply. In order to develop crop varieties with increased seed mineral concentration, a more detailed understanding of mineral translocation within the plant is required. By studying mineral accumulation in different organs within genetically diverse members of the same species, it may be possible to identify variable traits that modulate seed mineral concentration. We utilized two ecotypes (A17 and DZA315.16 of the model legume, Medicago truncatula, to study dry mass and mineral accumulation in the leaves, pod walls, and seeds during reproductive development. The pod wall dry mass was significantly different between the two ecotypes beginning at 12 days after pollination, whereas there was no significant difference in the average dry mass of individual seeds between the two ecotypes at any time point. There were also no significant differences in leaf dry mass between ecotypes; however, we observed expansion of A17 leaves during the first 21 days of pod development, while DZA315.16 leaves did not display a significant increase in leaf area. Mineral profiling of the leaves, pod walls, and seeds highlighted differences in accumulation patterns among minerals within each tissue as well as genotypic differences with respect to individual minerals. Because there were differences in the average seed number per pod, the total seed mineral content per pod was generally higher in A17 than DZA315.16. In addition, mineral partitioning to the seeds tended to be higher in A17 pods. These data revealed that mineral retention within leaves and/or pod walls might attenuate mineral accumulation within the seeds. As a result, strategies to increase seed mineral content should include approaches that will enhance export from these tissues.

  15. Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding

    Science.gov (United States)

    Maghsoudlou, Panagiotis; Georgiades, Fanourios; Smith, Holly; Milan, Anna; Shangaris, Panicos; Urbani, Luca; Loukogeorgakis, Stavros P.; Lombardi, Benedetta; Mazza, Giuseppe; Hagen, Charlotte; Sebire, Neil J.; Turmaine, Mark; Eaton, Simon; Olivo, Alessandro; Godovac-Zimmermann, Jasminka; Pinzani, Massimo; Gissen, Paul; De Coppi, Paolo

    2016-01-01

    Hepatic tissue engineering using decellularized scaffolds is a potential therapeutic alternative to conventional transplantation. However, scaffolds are usually obtained using decellularization protocols that destroy the extracellular matrix (ECM) and hamper clinical translation. We aim to develop a decellularization technique that reliably maintains hepatic microarchitecture and ECM components. Isolated rat livers were decellularized by detergent-enzymatic technique with (EDTA-DET) or without EDTA (DET). Histology, DNA quantification and proteomics confirmed decellularization with further DNA reduction with the addition of EDTA. Quantification, histology, immunostaining, and proteomics demonstrated preservation of extracellular matrix components in both scaffolds with a higher amount of collagen and glycosaminoglycans in the EDTA-DET scaffold. Scanning electron microscopy and X-ray phase contrast imaging showed microarchitecture preservation, with EDTA-DET scaffolds more tightly packed. DET scaffold seeding with a hepatocellular cell line demonstrated complete repopulation in 14 days, with cells proliferating at that time. Decellularization using DET preserves microarchitecture and extracellular matrix components whilst allowing for cell growth for up to 14 days. Addition of EDTA creates a denser, more compact matrix. Transplantation of the scaffolds and scaling up of the methodology are the next steps for successful hepatic tissue engineering. PMID:27159223

  16. Development of economically viable, highly integrated, highly modular SEGIS architecture.

    Energy Technology Data Exchange (ETDEWEB)

    Enslin, Johan (Petra Solar, Inc., South Plainfield, NJ); Hamaoui, Ronald (Petra Solar, Inc., South Plainfield, NJ); Gonzalez, Sigifredo; Haddad, Ghaith (Petra Solar, Inc., South Plainfield, NJ); Rustom, Khalid (Petra Solar, Inc., South Plainfield, NJ); Stuby, Rick (Petra Solar, Inc., South Plainfield, NJ); Kuran, Mohammad (Petra Solar, Inc., South Plainfield, NJ); Mark, Evlyn (Petra Solar, Inc., South Plainfield, NJ); Amarin, Ruba (Petra Solar, Inc., South Plainfield, NJ); Alatrash, Hussam (Petra Solar, Inc., South Plainfield, NJ); Bower, Ward Isaac; Kuszmaul, Scott S.; Sena-Henderson, Lisa; David, Carolyn; Akhil, Abbas Ali

    2012-03-01

    Initiated in 2008, the SEGIS initiative is a partnership involving the U.S. DOE, Sandia National Laboratories, private sector companies, electric utilities, and universities. Projects supported under the initiative have focused on the complete-system development of solar technologies, with the dual goal of expanding renewable PV applications and addressing new challenges of connecting large-scale solar installations in higher penetrations to the electric grid. Petra Solar, Inc., a New Jersey-based company, received SEGIS funds to develop solutions to two of these key challenges: integrating increasing quantities of solar resources into the grid without compromising (and likely improving) power quality and reliability, and moving the design from a concept of intelligent system controls to successful commercialization. The resulting state-of-the art technology now includes a distributed photovoltaic (PV) architecture comprising AC modules that not only feed directly into the electrical grid at distribution levels but are equipped with new functions that improve voltage stability and thus enhance overall grid stability. This integrated PV system technology, known as SunWave, has applications for 'Power on a Pole,' and comes with a suite of technical capabilities, including advanced inverter and system controls, micro-inverters (capable of operating at both the 120V and 240V levels), communication system, network management system, and semiconductor integration. Collectively, these components are poised to reduce total system cost, increase the system's overall value and help mitigate the challenges of solar intermittency. Designed to be strategically located near point of load, the new SunWave technology is suitable for integration directly into the electrical grid but is also suitable for emerging microgrid applications. SunWave was showcased as part of a SEGIS Demonstration Conference at Pepco Holdings, Inc., on September 29, 2011, and is presently

  17. Pollination with gamma-irradiated pollen and seed development in kiwifruit (Actinidia deliciosa var. deliciosa)

    International Nuclear Information System (INIS)

    Full text. The effects of pollen irradiation at 70 and 90 kr on seed set were studied in Actinidia deliciosa var. deliciosa. Pollination with irradiated pollen affected seed development and contents. Rising irradiation doses increased the percentages of empty seeds and decreased the percentages of seeds containing embryos with endosperm. Moreover, pollination with heavily irradiated pollen led to the formation of seeds containing the endosperm only. Embryo and endosperm size was also strongly influenced by irradiated pollen. The length of endosperms was reduced at all levels of pollen irradiation compared to the non-irradiated controls; the embryo development was conspicuously retarded. Cells in endosperm resulting from the treatments differed in the presence and number of starch grains. (author)

  18. A Modular Architecture for Developing Robots for Industrial Applications

    DEFF Research Database (Denmark)

    Faina, Andres; Orjales, Felix; Souto, Daniel;

    2015-01-01

    addresses the problem the other way around. In this line, we start by defining the industrial settings the architecture is aimed at and then extract the main features that would be required from a modular robotic architecture to operate successfully in this context. Finally, a particular heterogeneous......This chapter is concerned with proposing ways to make feasible the use of robots in many sectors characterized by dynamic and unstructured environments. In particular, we are interested in addressing the problem through a new approach, based on modular robotics, to allow the fast deployment...... of robotsto solve specific tasks. A series of authors have previously proposed modular architectures, albeit mostly in laboratory settings. For this reason, their designs were usually more focused on what could be built instead of what was necessary for industrial operations. The approach presented here...

  19. Soybean Seed Development: Fatty Acid and Phytohormone Metabolism and Their Interactions.

    Science.gov (United States)

    Nguyen, Quoc Thien; Kisiala, Anna; Andreas, Peter; Neil Emery, R J; Narine, Suresh

    2016-06-01

    Vegetable oil utilization is determined by its fatty acid composition. In soybean and other grain crops, during the seed development oil accumulation is important trait for value in food or industrial applications. Seed development is relatively short and sensitive to unfavorable abiotic conditions. These stresses can lead to a numerous undesirable qualitative as well as quantitative changes in fatty acid production. Fatty acid manipulation which targets a higher content of a specific single fatty acid for food or industrial application has gained more attention. Despite several successes in modifying the ratio of endogenous fatty acids in most domesticated oilseed crops, numerous obstacles in FA manipulation of seed maturation are yet to be overcome. Remarkably, connections with plant hormones have not been well studied despite their critical roles in the regulation and promotion of a plethora of processes in plant growth and development. While activities of phytohormones during the reproductive phase have been partially clarified in seed physiology, the biological role of plant hormones in oil accumulation during seed development has not been investigated. In this review seed development and numerous effects of abiotic stresses are discussed. After describing fatty acid and phytohormone metabolism and their interactions, we postulate that the endogenous plant hormones play important roles in fatty acid production in soybean seeds. PMID:27252591

  20. Dissecting the transcriptional regulatory network of seed and mesocarp development in peach

    OpenAIRE

    Zaffalon, Valerio

    2014-01-01

    Peach (Prunus persica) is one of the most important fleshy fruit crops worldwide and model species for drupe plant species. Peach fruit development is characterized by a tight relationship between seed and pericarp during the early stages, followed in later stages by an uncoupling in the pattern of development due to the lignification of the endocarp. Diverse peach cultivars may have fruit developmental periods of very different length, while having a similar development for the seed. Underst...

  1. The role of the testa during development and in establishment of dormancy of the legume seed

    Directory of Open Access Journals (Sweden)

    Petr eSmýkal

    2014-07-01

    Full Text Available Timing of seed germination is one of the key steps in plant life cycles. It determines the beginning of plant growth in natural or agricultural ecosystems. In the wild, many seeds exhibit dormancy and will only germinate after exposure to certain environmental conditions. In contrast, crop seeds germinate as soon as they are imbibed usually at planting time. These domestication-triggered changes represent adaptations to cultivation and human harvesting. Germination is one of the common sets of traits recorded in different crops and termed the domestication syndrome. Moreover, legume seed imbibition has a crucial role in cooking properties. Different seed dormancy classes exist among plant species. Physical dormancy (often called hardseededness, as found in legumes, involves the development of a water-impermeable seed coat, caused by the presence of phenolics- and suberin-impregnated layers of palisade cells. The dormancy release mechanism primarily involves seed responses to temperature changes in the habitat, resulting in testa permeability to water. The underlying genetic controls in legumes have not been identified yet. However, positive correlation was shown between phenolics content (e.g., pigmentation, the requirement for oxidation and the activity of catechol oxidase in relation to pea seed dormancy, while epicatechin levels showed a significant positive correlation with soybean hardseededness. MYB transcription factors, WD40 proteins and enzymes of the anthocyanin biosynthesis pathway were involved in seed testa colour in soybean, pea and Medicago, but were not tested directly in relation to seed dormancy. These phenolic compounds play important roles in defence against pathogens, as well as affecting the nutritional quality of products, and because of their health benefits, they are of industrial and medicinal interest. In this review, we discuss the role of the testa in mediating legume seed germination, with a focus on structural and

  2. The role of the testa during development and in establishment of dormancy of the legume seed.

    Science.gov (United States)

    Smýkal, Petr; Vernoud, Vanessa; Blair, Matthew W; Soukup, Aleš; Thompson, Richard D

    2014-01-01

    Timing of seed germination is one of the key steps in plant life cycles. It determines the beginning of plant growth in natural or agricultural ecosystems. In the wild, many seeds exhibit dormancy and will only germinate after exposure to certain environmental conditions. In contrast, crop seeds germinate as soon as they are imbibed usually at planting time. These domestication-triggered changes represent adaptations to cultivation and human harvesting. Germination is one of the common sets of traits recorded in different crops and termed the "domestication syndrome." Moreover, legume seed imbibition has a crucial role in cooking properties. Different seed dormancy classes exist among plant species. Physical dormancy (often called hardseededness), as found in legumes, involves the development of a water-impermeable seed coat, caused by the presence of phenolics- and suberin-impregnated layers of palisade cells. The dormancy release mechanism primarily involves seed responses to temperature changes in the habitat, resulting in testa permeability to water. The underlying genetic controls in legumes have not been identified yet. However, positive correlation was shown between phenolics content (e.g., pigmentation), the requirement for oxidation and the activity of catechol oxidase in relation to pea seed dormancy, while epicatechin levels showed a significant positive correlation with soybean hardseededness. myeloblastosis family of transcription factors, WD40 proteins and enzymes of the anthocyanin biosynthesis pathway were involved in seed testa color in soybean, pea and Medicago, but were not tested directly in relation to seed dormancy. These phenolic compounds play important roles in defense against pathogens, as well as affecting the nutritional quality of products, and because of their health benefits, they are of industrial and medicinal interest. In this review, we discuss the role of the testa in mediating legume seed germination, with a focus on structural

  3. Quantitative phosphoproteomic analysis of early seed development in rice (Oryza sativa L.).

    Science.gov (United States)

    Qiu, Jiehua; Hou, Yuxuan; Tong, Xiaohong; Wang, Yifeng; Lin, Haiyan; Liu, Qing; Zhang, Wen; Li, Zhiyong; Nallamilli, Babi R; Zhang, Jian

    2016-02-01

    Rice (Oryza sativa L.) seed serves as a major food source for over half of the global population. Though it has been long recognized that phosphorylation plays an essential role in rice seed development, the phosphorylation events and dynamics in this process remain largely unknown so far. Here, we report the first large scale identification of rice seed phosphoproteins and phosphosites by using a quantitative phosphoproteomic approach. Thorough proteomic studies in pistils and seeds at 3, 7 days after pollination resulted in the successful identification of 3885, 4313 and 4135 phosphopeptides respectively. A total of 2487 proteins were differentially phosphorylated among the three stages, including Kip related protein 1, Rice basic leucine zipper factor 1, Rice prolamin box binding factor and numerous other master regulators of rice seed development. Moreover, differentially phosphorylated proteins may be extensively involved in the biosynthesis and signaling pathways of phytohormones such as auxin, gibberellin, abscisic acid and brassinosteroid. Our results strongly indicated that protein phosphorylation is a key mechanism regulating cell proliferation and enlargement, phytohormone biosynthesis and signaling, grain filling and grain quality during rice seed development. Overall, the current study enhanced our understanding of the rice phosphoproteome and shed novel insight into the regulatory mechanism of rice seed development. PMID:26613898

  4. Strategies for seed system development in sub-Saharan Africa: a study of Kenya, Malawi, Zambia, and Zimbabwe

    Directory of Open Access Journals (Sweden)

    Robert Tripp

    2006-08-01

    Full Text Available Seed systems in Kenya, Malawi, Zambia, and Zimbabwe were studied jointly by ODI, ICRISAT, and national researchers. The study examined various aspects - the nature of seed demand, particularly for new varieties; local-level seed provision and farmer-to-farmer diffusion; the commercial seed sector; community level projects; emergency seed distribution programs; seed policy and regulatory framework; and the role of public-sector research. The study also provides specific recommendations to develop the seed sector. For example, public research programs must invest more heavily in variety promotion and production of breeder and foundation seed. Seed certification should be made voluntary, and variety registration simplified. Emergency seed programs should pay greater attention to variety adaptation and seed quality; and should support local seed enterprises, not compete with them. Seed companies should strengthen their retail networks. NGO seed projects should consider marketing and sustainability issues more carefully. Donor-funded projects, which currently operate in isolation from each other, must be integrated into a coherent, long-term, nationally-directed seed strategy.

  5. Timing of fire relative to seed development may enable non-serotinous species to recolonize from the aerial seed banks of fire-killed trees

    Directory of Open Access Journals (Sweden)

    S. T. Michaletz

    2013-07-01

    Full Text Available The existence of non-serotinous, non-sprouting species in fire regimes where serotiny confers an adaptive advantage is puzzling, particularly when these species recruit poorly from soil seed banks or from burn edges. In this paper, white spruce (Picea glauca (Moench Voss was used to show how the timing of fire relative to seed development may permit non-serotinous species to recolonize burned areas from the aerial seed banks of fire-killed trees. To estimate survival of seeds within closed cones during crown fires, cone heating was simulated using a one-dimensional conduction model implemented in a three-dimensional computational fluid dynamics fire behavior model. To quantify the area burned when germinable seed would be contained within closed cones during a mast year, empirical fire occurrence and seed development (germinability and cone opening data were compared for multiple locations across the white spruce range. Approximately 12% of cones contained viable seed following crown fire simulations (0.072 m s−1 mean spread rate; 9147 kW m−1 mean intensity, and roughly half of the historical area burned resulted from fires that occurred when closed cones would contain germinable seed. Together, these results suggest that non-serotinous species may recolonize burned areas from in situ aerial seed banks, and that this may be an important cause of their existence in fire regimes to which they otherwise seem poorly suited.

  6. Germination and Seedling Development of Seeds from Different Parkia biglobosa (Jacq G. Don Trees

    Directory of Open Access Journals (Sweden)

    Christiana O. ADEYEMI

    2013-02-01

    Full Text Available The effect of daylight, continuous illumination and acid scarification on the seed germination and seedling vegetative growth (epicotyl and hypocotyl lengths, and number of secondary roots of different Parkia biglobosawere investigated in the Plant Physiology Laboratory University of Ilorin, Ilorin Kwara State Nigeria. Seeds from two out of the twenty six Parkia tree samples (trees B and T germinated within 24 hours of planting in the daylight germination study while seeds from another tree (Q did not germinate until the third week after planting (3WAP. Some seeds have higher germination percentage both in the daylight (preliminary germination study and in the continuous light (illuminated study. The treatment with concentrated Sulphric acid (conc. H2SO4was effective in breaking the seed dormancy as seeds from eight (8 trees produced one hundred percent (100% germination. At p= 0.05 the length of epicotyl and hypocoty1 lengths were significantly different as seedling vegetative growth were long in the seedlings from the daylight experiment than the continuous light experiment. The vegetative growths of the seedlings from the scarified seed were longer at 15min of scarification in all except in trees F and Z. It was observed that the time of scarification affect the both seed germination and seedling development.

  7. Design and development of desiccant seed dryer with airflow inversion and recirculation.

    Science.gov (United States)

    Gill, R S; Singh, Sukhmeet; Singh, Parm Pal

    2014-11-01

    A desiccant seed dryer has been developed to dry seed in deep bed at safe temperatures for good shelf life and germination. The dryer consists of two chambers viz., air conditioning control unit and seed drying chamber. It operates in seed drying mode and desiccant regeneration mode. It has provision for recirculation of the drying air to minimise the moisture removal from drying air. Also, it has provision of airflow inversion through deep seed bed for uniform drying. Moisture removal from drying air has been done using silica gel desiccant. Chilly 'Punjab Surakh', Chilly 'Punjab Guchhedaar', Paddy, Coriander, Fenugreek and Radish seeds was dried with hot air at 38 °C from initial moisture content of 26.9 to 5 % (wb) in 2 h, 46.52 to 4.19 % (wb) in 4.25 h, 13.3 to 2.61 % (wb) in 4 h, 13.4 to 10.08 % (wb) in 3 h, 12.4 to 8.22 % (wb) in 4¼ h and 10.6 to 6.08 % (wb) in 4 h respectively. The statistical analysis based on paired t-test showed that seed drying in this dryer has no adverse effect on seed germination. PMID:26396340

  8. Effect of seed on ripening control components during avocado fruit development.

    Science.gov (United States)

    Hershkovitz, Vera; Friedman, Haya; Goldschmidt, Eliezer E; Feygenberg, Oleg; Pesis, Edna

    2011-12-15

    Seedless avocado fruit are produced alongside seeded fruit in the cultivar Arad, and both reach maturity at the same time. Using this system, it was possible to show that avocado seed inhibits the ripening process: seedless fruits exhibited higher response to exogenous ethylene already at the fruitlet stage, and also at the immature and mature fruit stages. They produced higher CO₂ levels, and the ethylene peak was apparent at the fruitlet stage of seedless fruit, but not of seeded ones. The expression levels of PaETR, PaERS1 and PaCTR1 on the day of harvest at all developmental stages were very similar between seeded and seedless fruit, except that PaCTR1 was higher in seedless fruit only at very early stages. This expression pattern suggests that the seed does not have an effect on components of the ethylene response pathway when fruits are just picked. The expression of MADS-box genes, PaAG1 and PaAGL9, preceded the increase in ethylene production of mature seeded fruit, but not at earlier stages. However, only PaAGL9 was induced in seedless fruit at early stages of development. Taken together, these data suggest that these genes are perhaps involved in climacteric response in seeded fruit, and the seed is responsible for their induction at normal fruit ripening. PMID:21930327

  9. Dynamics of storage reserve deposition during Brassica rapa L. pollen and seed development in microgravity

    Science.gov (United States)

    Kuang, A.; Popova, A.; McClure, G.; Musgrave, M. E.

    2005-01-01

    Pollen and seeds share a developmental sequence characterized by intense metabolic activity during reserve deposition before drying to a cryptobiotic form. Neither pollen nor seed development has been well studied in the absence of gravity, despite the importance of these structures in supporting future long-duration manned habitation away from Earth. Using immature seeds (3-15 d postpollination) of Brassica rapa L. cv. Astroplants produced on the STS-87 flight of the space shuttle Columbia, we compared the progress of storage reserve deposition in cotyledon cells during early stages of seed development. Brassica pollen development was studied in flowers produced on plants grown entirely in microgravity on the Mir space station and fixed while on orbit. Cytochemical localization of storage reserves showed differences in starch accumulation between spaceflight and ground control plants in interior layers of the developing seed coat as early as 9 d after pollination. At this age, the embryo is in the cotyledon elongation stage, and there are numerous starch grains in the cotyledon cells in both flight and ground control seeds. In the spaceflight seeds, starch was retained after this stage, while starch grains decreased in size in the ground control seeds. Large and well-developed protein bodies were observed in cotyledon cells of ground control seeds at 15 d postpollination, but their development was delayed in the seeds produced during spaceflight. Like the developing cotyledonary tissues, cells of the anther wall and filaments from the spaceflight plants contained numerous large starch grains, while these were rarely seen in the ground controls. The tapetum remained swollen and persisted to a later developmental stage in the spaceflight plants than in the ground controls, even though most pollen grains appeared normal. These developmental markers indicate that Brassica seeds and pollen produced in microgravity were physiologically younger than those produced in 1 g

  10. Architectural Prototyping

    DEFF Research Database (Denmark)

    Bardram, Jakob; Christensen, Henrik Bærbak; Hansen, Klaus Marius

    2004-01-01

    A major part of software architecture design is learning how specific architectural designs balance the concerns of stakeholders. We explore the notion of "architectural prototypes", correspondingly architectural prototyping, as a means of using executable prototypes to investigate stakeholders......' concerns with respect to a system under development. An architectural prototype is primarily a learning and communication vehicle used to explore and experiment with alternative architectural styles, features, and patterns in order to balance different architectural qualities. The use of architectural...... prototypes in the development process is discussed, and we argue that such prototypes can play a role throughout the entire process. The use of architectural prototypes is illustrated by three distinct cases of creating software systems. We argue that architectural prototyping can provide key insights that...

  11. Architectural prototyping

    DEFF Research Database (Denmark)

    Bardram, Jakob Eyvind; Christensen, Henrik Bærbak; Hansen, Klaus Marius

    2004-01-01

    A major part of software architecture design is learning how specific architectural designs balance the concerns of stakeholders. We explore the notion of "architectural prototypes", correspondingly architectural prototyping, as a means of using executable prototypes to investigate stakeholders......' concerns with respect to a system under development. An architectural prototype is primarily a learning and communication vehicle used to explore and experiment with alternative architectural styles, features, and patterns in order to balance different architectural qualities. The use of architectural...... prototypes in the development process is discussed, and we argue that such prototypes can play a role throughout the entire process. The use of architectural prototypes is illustrated by three distinct cases of creating software systems. We argue that architectural prototyping can provide key insights that...

  12. EVALUATION OF SOYASAPONIN, ISOFLAVONE, PROTEIN, LIPID, AND FREE SUGAR ACCUMULATION IN DEVELOPING SOYBEAN SEEDS

    Science.gov (United States)

    A combination of analytical techniques were used to examine and quantify seed compositional components (protein content, lipid content, carbohydrates, isoflavones, and saponins) during bean development and maturation in two Korean soy cultivars. Protein accumulation was rapid during reproductive st...

  13. 31P NMR study of phosphate metabolites in intact developing seeds of wheat, soybean and mustard

    International Nuclear Information System (INIS)

    The study of 31P NMR spectra of intact developing seeds of wheat, soybean and mustard and its possible use for assessing the relative degree of hypoxia under in vivo conditions are reported. 7 refs., 2 figs

  14. Evolution of the Systems Engineering Education Development (SEED) Program at NASA Goddard Space Flight Center

    Science.gov (United States)

    Bagg, Thomas C., III; Brumfield, Mark D.; Jamison, Donald E.; Granata, Raymond L.; Casey, Carolyn A.; Heller, Stuart

    2003-01-01

    The Systems Engineering Education Development (SEED) Program at NASA Goddard Space Flight Center develops systems engineers from existing discipline engineers. The program has evolved significantly since the report to INCOSE in 2003. This paper describes the SEED Program as it is now, outlines the changes over the last year, discusses current status and results, and shows the value of human systems and leadership skills for practicing systems engineers.

  15. Developing a partcipatory approach to seed production and varietal selection

    OpenAIRE

    Jones, H.; Clarke, S M; Haigh, Z; Hinchsliffe, K; Wolfe, M.S; Thomas, J.; Gibbon, D.; Harris, F.; Lyon, F

    2006-01-01

    The performance of UK winter wheat varieties was tested under organic conditions involving farmer participation. Three breadmaking varieties (Hereward, Solstice and Xi19) and their mixture (1:1:1) were grown at 19 UK farms in 2003/04 and 2004/05. The variability of productivity on organic farms was illustrated with more variation among farm sites than among varieties. Seed health was generally high over all sites. Although the trials were successful, more time was needed at project initiation...

  16. Disasters and development in agricultural input markets: bean seed markets in Honduras after Hurricane Mitch.

    Science.gov (United States)

    Mainville, Denise Y

    2003-06-01

    The bulk of developing countries' populations and poor depend on agriculture for food and income. While rural economies and people are generally the most severely affected by natural disasters, little is known about how disasters and subsequent relief activities affect agricultural markets with differing levels of development. The article addresses this gap, drawing evidence from bean seed markets in Honduras after Hurricane Mitch. Case studies are used to address hypotheses about a disaster's effects on supply and demand in seed markets, farmers' responses and the performance of relief interventions in markets showing differing levels of development. The results show the importance of tailoring relief interventions to the markets that they will affect and to the specific effects of a disaster; the potential to use local and emerging seed distribution channels in a relief intervention; and opportunities for relief activities to strengthen community seed systems. PMID:12825438

  17. An Architecture to Develop Multimodal Educative Applications with Chatbots

    OpenAIRE

    David Griol; Zoraida Callejas

    2013-01-01

    Animated characters are beginning to be used as pedagogical tools, as they have the power to capture students’ attention and foster their motivation for discovery and learning. However, in order for them to be widely employed and accepted as a learning resource, they must be easy to use and friendly. In this paper we present an architecture that facilitates building interactive pedagogical chatbots that can interact with students in natural language. Our proposal provides a modular and scalab...

  18. Service-Oriented Architecture concept for intelligence information system development

    OpenAIRE

    Ackoski, Jugoslav; Trajkovik, Vladimir; Davcev, Danco

    2011-01-01

    This paper presents an idea for Service Oriented Architecture (SOA) approach in prototype of Intelligence Information System (IIS). IIS prototype, based on SOA, could offer better coordination among institutions involved in intelligence thus providing increase of intelligence effectiveness. This approach can serve as a foundation for the establishment of the Integrated Intelligence System, which is based on services as software components. In this paper, we propose fiv...

  19. Specifics of projects in the area of Enterprise architecture development

    OpenAIRE

    KOZNOV D.V.; ARZUMANYAN M.YU.; ORLOV YU.V.; DEREVYANKO M.A.; ROMANOVSKY K.YU.; SIDORINA A.A.

    2015-01-01

    Enterprise Architecture (EA) is a discipline for business and IT system management, describing the fundamental artifacts of business and IT. Introducing EA activities in a company is done via special projects (referred to as EAM projects). While much research focuses on EA as is, EAM projects are not yet considered properly. This paper deals with EAM projects as a special kind of IT project. The definition of an EAM project is suggested using the IT solution concept of the Microsoft Solution ...

  20. Global analysis of gene expression profiles in developing physic nut (Jatropha curcas L. seeds.

    Directory of Open Access Journals (Sweden)

    Huawu Jiang

    Full Text Available BACKGROUND: Physic nut (Jatropha curcas L. is an oilseed plant species with high potential utility as a biofuel. Furthermore, following recent sequencing of its genome and the availability of expressed sequence tag (EST libraries, it is a valuable model plant for studying carbon assimilation in endosperms of oilseed plants. There have been several transcriptomic analyses of developing physic nut seeds using ESTs, but they have provided limited information on the accumulation of stored resources in the seeds. METHODOLOGY/PRINCIPAL FINDINGS: We applied next-generation Illumina sequencing technology to analyze global gene expression profiles of developing physic nut seeds 14, 19, 25, 29, 35, 41, and 45 days after pollination (DAP. The acquired profiles reveal the key genes, and their expression timeframes, involved in major metabolic processes including: carbon flow, starch metabolism, and synthesis of storage lipids and proteins in the developing seeds. The main period of storage reserves synthesis in the seeds appears to be 29-41 DAP, and the fatty acid composition of the developing seeds is consistent with relative expression levels of different isoforms of acyl-ACP thioesterase and fatty acid desaturase genes. Several transcription factor genes whose expression coincides with storage reserve deposition correspond to those known to regulate the process in Arabidopsis. CONCLUSIONS/SIGNIFICANCE: The results will facilitate searches for genes that influence de novo lipid synthesis, accumulation and their regulatory networks in developing physic nut seeds, and other oil seeds. Thus, they will be helpful in attempts to modify these plants for efficient biofuel production.

  1. Identification of desiccation tolerance transcripts potentially involved in rape (Brassica napus L.) seeds development and germination.

    Science.gov (United States)

    Lang, Sirui; Liu, Xiaoxia; Ma, Gang; Lan, QinYing; Wang, Xiaofeng

    2014-10-01

    To investigate regulatory processes and protective mechanisms leading to desiccation tolerance (DT) in seeds, cDNA amplified fragment length polymorphism (cDNA-AFLP) in conjunction with 128 primer combinations was used to detect differential gene expression in rape seeds in response to DT during seed development and germination. We obtained approximately 8000 transcript-derived fragments (TDFs), of which 394 TDFs with differential expression patterns ("sustained expression", "up-regulated", "couple with seed DT", and "down-regulated") were excised from gels and re-amplified by polymerase chain reaction (PCR). After sequencing and comparison with the National Center for Biotechnology Information database, 176 TDFs presented significant similarity with known genes that could be classified into the following categories: metabolism and energy, stress resistance and defense, storage, signal transduction, and other functional categories. Using semiquantitative reverse-transcription PCR and real-time PCR approaches, the significance of the differences was further confirmed in fresh seeds and dehydrated seeds. The genes that encode superoxide dismutase, peroxiredoxin, caleosin, oleosin S3, steroleosin, late embryogenesis abundant protein, glutathione reductase, β-glucosidase, S23 transcriptional repressor, and some heat-shock proteins could be associated with DT. The results of this study will aid in the identification of candidate genes for future experiments that seek to understand seed DT. PMID:25221920

  2. Development of scaffold architectures and heterotypic cell systems for hepatocyte transplantation

    Science.gov (United States)

    Alzebdeh, Dalia Abdelrahim

    In vitro assembly of functional liver tissue is needed to enable the transplantation of tissue-engineered livers. In addition, there is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of sizable implantable liver systems is currently limited by the difficulty of assembling three dimensional hepatocyte cultures of a useful size, while maintaining full cell viability, an issue which is closely related to the high metabolic rate of hepatocytes. In this study, we first compared two designs of highly porous chitosan-heparin scaffolds seeded with hepatocytes in dynamic perfusion bioreactor systems. The aim was to promote cell seeding efficiency by effectively entrapping 100 million hepatocytes at high density. We found that scaffolds with radially tapering pore architecture had highly efficient cell entrapment that maximized donor hepatocyte utilization, compared to alternate pore structures. Hepatocytes showed higher seeding efficiency and metabolic function when seeded as single cell suspensions as opposed to pre-formed, 100microm aggregates. Seeding efficiency was found to increase with flow rate, with single cell and aggregate suspension exhibiting different optimal flow rates. However, metabolic performance results indicated significant shear damage to cells at high efficiency flow rates. To better maintain hepatocyte basement membrane and cell polarity, spheroid co-cultures with mesenchymal stem cells (MSC) were investigated. Hepatocytes and MSCs were seeded in three different architectures in an effort to optimize the spatial arrangement of the two cell types. MSC co-culture greatly enhanced hepatocyte metabolic function in agitated cultures. Interestingly, the effects of diffusion limitations in spheroid culture, coupled with shear damage and subsequent removal of outer hepatocyte layers produced a defined oscillation of urea production rates in certain co-culture arrangements. A

  3. Effect of seed coat on the seed germination and seedling development of Calophyllum brasiliense Cambess. (Clusiaceae)

    OpenAIRE

    Valquíria Aparecida Mendes de Jesus; Alessandro Lucca Braccini; Carlos Alberto Scapim; Fábio Lúcio Santos; Giovanna Emanuelle Goncalves Mariucci; Andréia Kazumi Suzukawa

    2014-01-01

    This work aimed to study the effect of the Calophyllum brasiliense seed coat on the seed germination process. To this end, three experiments were conducted in laboratory, greenhouse and screenhouse. From a total of six treatments, five are related to the seed coat (mechanical scarification; mechanical scarification followed by 2 hours in water, chemical scarification, hot water immersion and complete seed coat removal) and one control. Laboratory and greenhouse experiments were conducted in a...

  4. Lipid analysis of developing Camelina sativa seeds and cultured embryos.

    Science.gov (United States)

    Pollard, Mike; Martin, Tina M; Shachar-Hill, Yair

    2015-10-01

    Camelina sativa is a cultivated oilseed rich in triacylglycerols containing oleic, linoleic, α-linolenic and eicosenoic acids. As it holds promise as a model species, its lipid synthesis was characterized in vivo and in culture. Lipid accumulates at a maximum rate of about 26 μg/day/seed (11.5 mg lipid/day/g fresh seed weight), a rate comparable with other oilseeds. Noteworthy is a late stage surge in α-linolenic acid accumulation. Small amounts of unusual epoxy and hydroxy fatty acids are also present in the triacylglycerols. These include 15,16-epoxy- and 15-hydroxy-octadecadienoic acids and homologous series of ω7-hydroxy-alk-ω9-enoic and ω9/10-hydroxy-alkanoic acids. Mid-maturation embryos cultured in vitro have growth and lipid deposition rates and fatty acid compositions that closely match that of seeds, but extended culture periods allow these rates to rise and surpass those observed in planta. Optimized thin layer chromatography systems for characterization of labeled products from acetate or glycerol labeling are described. Glycerol label is only found in acylglycerols, largely as the intact glyceryl backbone, but acetate can label acyl groups and sterols, the latter to a much higher relative specific activity. This presumably occurs because mevalonic acid precursor is derived from the non-plastid pool of acetyl-CoA that is also the source for malonyl-CoA to drive FAE1-dependent chain elongation. Particular attention has been paid to the separation of sterols and diacylglycerols, and to hydrogenation of triacylglycerols to simplify their analysis. These improved methods will allow more accurate analyses of the fluxes of lipid metabolism in cultured plant embryos. PMID:26262674

  5. Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut

    OpenAIRE

    Wei ZHU; Chen, Xiaoping; Li, Haifen; Zhu, Fanghe; Hong, Yanbin; Rajeev K. Varshney; Liang, Xuanqiang

    2014-01-01

    The peanut is a special plant for its aerial flowering but subterranean fructification. The failure of peg penetration into the soil leads to form aerial pod and finally seed abortion. However, the mechanism of seed abortion during aerial pod development remains obscure. Here, a comparative transcriptome analysis between aerial and subterranean pods at different developmental stages was produced using a customized NimbleGen microarray representing 36,158 unigenes. By comparing 4 consecutive t...

  6. Plant growth analysis and seed vigor expression: effects of soil waterlogging during rye plant development

    OpenAIRE

    Tiago Pedó; Emanuela Garbin Martinazzo; Tiago Zanatta Aumonde; Francisco Amaral Villela

    2015-01-01

    This study aimed to evaluate the growth, assimilate partitioning and seed vigor expression of rye seeds subjected to waterlogging during development. The experimental design was completely randomized with six replications and three treatments. The treatments involved different waterlogging periods: a) no waterlogging; b) single waterlogging of three days; and c) two soil waterlogging periods of three days. The plants were collected at regular intervals of 14 days after emergence until the end...

  7. Hardware additions to microprocessor architecture aid software development

    Science.gov (United States)

    Sievers, M. W.

    1976-01-01

    An address trap (breakpoint) mechanism and last-in-first-out (LIFO) address stack are suggested as two additions to the basic microprocessor architecture whose functions are solely to aid the programmer. These devices provide the programmer with the ability to specify address breakpoints and to trace program execution back through N instructions, where N is the depth of the stack. Both devices, plus interface logic and buffering, have been designed for an INTEL 8080-based system using approximately 25 integrated-circuit packages.

  8. Changes in activation sequence of embryonic chicken atria correlate with developing myocardial architecture

    Czech Academy of Sciences Publication Activity Database

    Sedmera, David; Wessels, A.; Thompson, R. P.; Gourdie, R. G.

    Malaga, 2006. s. 6. [European Cardiovascular Development and Pathology Meeting. 18.03.2006-21.03.2006, Malaga] Institutional research plan: CEZ:AV0Z50450515 Keywords : myocardial architecture Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  9. Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

    Directory of Open Access Journals (Sweden)

    Ayeh Kwadwo

    2011-11-01

    Full Text Available Abstract Background The def mutant pea (Pisum sativum L showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations. Findings Pod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either the distal and proximal ends of the pod did not show any difference. The growth factors of seed fresh weight (FW, width of funicles (WFN, seed width (SW and seed height (SH were highly correlated and their relationships were determined in both wild type and def mutant peas. The coefficient of determination R2 values for the relationship between WFN and FW, SW and SH and their various interactions were higher for the def dwarf type. Stepwise multiple regression analysis showed that variation of WFN was associated with SH and SW. Pearson's chi square analysis revealed that the inheritance and segregation of the Def locus in 3:1 ratio was significant in two F2 populations. Structural analysis of the F3 population was used to confirm the inheritance status of the Def locus in F2 heterozygote plants. Conclusions This study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ or an abscission-less zone (ALZ forming in wild type and mutant lines respectively. The single major gene (Def controlling this phenotype was monogenic and def mutants were characterized and controlled by the homozygous recessive def allele that showed no palisade layers in the hilum region of the seed coat.

  10. Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L. Taub

    Directory of Open Access Journals (Sweden)

    Dixon Richard A

    2007-11-01

    Full Text Available Abstract Background Guar, Cyamopsis tetragonoloba (L. Taub, is a member of the Leguminosae (Fabaceae family and is economically the most important of the four species in the genus. The endosperm of guar seed is a rich source of mucilage or gum, which forms a viscous gel in cold water, and is used as an emulsifier, thickener and stabilizer in a wide range of foods and industrial applications. Guar gum is a galactomannan, consisting of a linear (1→4-β-linked D-mannan backbone with single-unit, (1→6-linked, α-D-galactopyranosyl side chains. To better understand regulation of guar seed development and galactomannan metabolism we created cDNA libraries and a resulting EST dataset from different developmental stages of guar seeds. Results A database of 16,476 guar seed ESTs was constructed, with 8,163 and 8,313 ESTs derived from cDNA libraries I and II, respectively. Library I was constructed from seeds at an early developmental stage (15–25 days after flowering, DAF, and library II from seeds at 30–40 DAF. Quite different sets of genes were represented in these two libraries. Approximately 27% of the clones were not similar to known sequences, suggesting that these ESTs represent novel genes or may represent non-coding RNA. The high flux of energy into carbohydrate and storage protein synthesis in guar seeds was reflected by a high representation of genes annotated as involved in signal transduction, carbohydrate metabolism, chaperone and proteolytic processes, and translation and ribosome structure. Guar unigenes involved in galactomannan metabolism were identified. Among the seed storage proteins, the most abundant contig represented a conglutin accounting for 3.7% of the total ESTs from both libraries. Conclusion The present EST collection and its annotation provide a resource for understanding guar seed biology and galactomannan metabolism.

  11. SCB1, a BURP-domain protein gene, from developing soybean seed coats.

    Science.gov (United States)

    Batchelor, Anthea K; Boutilier, Kim; Miller, S Shea; Hattori, Jiro; Bowman, Lu Anne; Hu, Ming; Lantin, Sylviane; Johnson, Douglas A; Miki, Brian L A

    2002-08-01

    We describe a gene, SCB1 (Seed Coat BURP-domain protein 1), that is expressed specifically within the soybean (Glycine max [L.] Merrill) seed coat early in its development. Northern blot analysis and mRNA in situ hybridization revealed novel patterns of gene expression during seed development. SCB1 mRNA accumulated first within the developing thick-walled parenchyma cells of the inner integument and later in the thick- and thin-walled parenchyma cells of the outer integument. This occurred prior to the period of seed coat maturation and seed filling and before either of the layers started to degrade. SCB1 may therefore play a role in the differentiation of the seed coat parenchyma cells. In addition, the protein product appears to be located within cell walls. The SCB1 gene codes for a new member of a class of modular proteins that possess a carboxy-terminal BURP domain and a variety of different repeated sequences. The sequence of the genomic clone revealed the insertion of a Tgm transposable element in the upstream promoter region but it is not certain whether it contributes to the tissue-specific pattern of SCB1 expression. PMID:12172833

  12. Integration of theory courses and design studio in architectural education using sustainable development

    OpenAIRE

    Bozkurt Eray

    2016-01-01

    Sustainable development has been approved by many experts as the only suitable model for the growth of the next generation. Education has a valuable role to distribute sustainable approach to all subject fields like architecture. Sustainable development is gaining a central position in the conceptual framework of architectural education in universities. Universities are complex organizations that are frequently quite stable in the short term, but inevitably evolving over the longer term in re...

  13. Expression of genes controlling unsaturated fatty acids biosynthesis and oil deposition in developing seeds of Sacha inchi (Plukenetia volubilis L.).

    Science.gov (United States)

    Wang, Xiaojuan; Liu, Aizhong

    2014-10-01

    Sacha inchi (Plukenetia volubilis L., Euphorbiaceae) seed oil is rich in α-linolenic acid, a kind of n-3 fatty acids with many health benefits. To discover the mechanism underlying α-linolenic acid accumulation in sacha inchi seeds, preliminary research on sacha inchi seed development was carried out from one week after fertilization until maturity, focusing on phenology, oil content, and lipid profiles. The results suggested that the development of sacha inchi seeds from pollination to mature seed could be divided into three periods. In addition, investigations on the effect of temperature on sacha inchi seeds showed that total oil content decreased in the cool season, while unsaturated fatty acid and linolenic acid concentrations increased. In parallel, expression profiles of 17 unsaturated fatty acid related genes were characterized during seed development and the relationships between gene expression and lipid/unsaturated fatty acid accumulation were discussed. PMID:25119487

  14. Using of new possibilities of Fermi architecture by development og GPGPU programs

    International Nuclear Information System (INIS)

    Description of additional functions of hardware and software, which are presented in the structure of new architecture of FERMI graphic processors made by company NVIDIA, was given. Recommendations of their use within the realization of algorithms of scientific and technical calculations by means of the graphic processors were given. Application of the new possibilities of FERMI architecture and CUDA technologies (Compute Unified Device Architecture - unified hardware-software decision for parallel calculations on GPU) of NVIDIA Company was described. It was done for time reduction of applications' development which is using possibilities of GPGPU for acceleration of data processing

  15. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 is essential for thermoinhibition of lettuce seed germination but not for seed development or stress tolerance.

    Science.gov (United States)

    Huo, Heqiang; Dahal, Peetambar; Kunusoth, Keshavulu; McCallum, Claire M; Bradford, Kent J

    2013-03-01

    Thermoinhibition, or failure of seeds to germinate at warm temperatures, is common in lettuce (Lactuca sativa) cultivars. Using a recombinant inbred line population developed from a lettuce cultivar (Salinas) and thermotolerant Lactuca serriola accession UC96US23 (UC), we previously mapped a quantitative trait locus associated with thermoinhibition of germination to a genomic region containing a gene encoding a key regulated enzyme in abscisic acid (ABA) biosynthesis, 9-cis-EPOXYCAROTENOID DIOXYGENASE4 (NCED4). NCED4 from either Salinas or UC complements seeds of the Arabidopsis thaliana nced6-1 nced9-1 double mutant by restoring germination thermosensitivity, indicating that both NCED4 genes encode functional proteins. Transgenic expression of Salinas NCED4 in UC seeds resulted in thermoinhibition, whereas silencing of NCED4 in Salinas seeds led to loss of thermoinhibition. Mutations in NCED4 also alleviated thermoinhibition. NCED4 expression was elevated during late seed development but was not required for seed maturation. Heat but not water stress elevated NCED4 expression in leaves, while NCED2 and NCED3 exhibited the opposite responses. Silencing of NCED4 altered the expression of genes involved in ABA, gibberellin, and ethylene biosynthesis and signaling pathways. Together, these data demonstrate that NCED4 expression is required for thermoinhibition of lettuce seeds and that it may play additional roles in plant responses to elevated temperature. PMID:23503626

  16. Seedling development and evaluation of genetic stability of cryopreserved Dendrobium hybrid mature seeds.

    Science.gov (United States)

    Galdiano, Renato Fernandes; de Macedo Lemos, Eliana Gertrudes; de Faria, Ricardo Tadeu; Vendrame, Wagner Aparecido

    2014-03-01

    Vitrification, a simple, fast, and recommended cryopreservation method for orchid germplasm conservation, was evaluated for Dendrobium hybrid "Dong Yai" mature seeds. The genetic stability of regenerated seedlings was also evaluated using flow cytometry. Mature seeds from this hybrid were submitted to plant vitrification solution (PVS2) for 0, 0.5, 1, 2, 3, 4, 5, or 6 h at 0 °C. Subsequently, they were plunged into liquid nitrogen (LN) at -196 °C for 1 h and recovered in half-strength Murashige and Skoog culture medium (1/2 MS), and seed germination was evaluated after 30 days. Seeds directly submitted to LN did not germinate after cryopreservation. Seeds treated with PVS2 between 1 and 3 h presented the best germination (between 51 and 58%), although longer exposure to PVS2 returned moderated germination (39%). Germinated seeds were further subcultured in P-723 culture medium and developed whole seedlings in vitro after 180 days, with no abnormal characteristics, diseases, or nutritional deficiencies. Seedlings were successfully acclimatized under greenhouse conditions with over 80% survival. Flow cytometry analysis revealed no chromosomal changes on vitrified seedlings, as well as seedlings germinated from the control treatment (direct exposure to LN). These findings indicate that vitrification is a feasible and safe germplasm cryopreservation method for commercial Dendrobium orchid hybrid conservation. PMID:24402568

  17. Architecture & Environment

    Science.gov (United States)

    Erickson, Mary; Delahunt, Michael

    2010-01-01

    Most art teachers would agree that architecture is an important form of visual art, but they do not always include it in their curriculums. In this article, the authors share core ideas from "Architecture and Environment," a teaching resource that they developed out of a long-term interest in teaching architecture and their fascination with the…

  18. Study and development of an Iridium-192 seed for use in ophthalmic cancer

    International Nuclear Information System (INIS)

    Even ocular tumors are not among the cases with a higher incidence, they affect the population, especially children. The Institute of Energy and Nuclear Research (IPEN-CNEN/SP) in partnership with Escola Paulista de Medicina (UNIFESP), created a project to develop and implement a alternative treatment for ophthalmic cancer that use brachytherapy iridium-192 seeds. The project arose by reason of the Escola Paulista treat many cancer cases within the Unified Health System (SUS) and the research experience of sealed radioactive sources group at IPEN. The methodology was developed from the available infrastructure and the experience of researchers. The prototype seed presents with a core (192-iridium alloy of iridium-platinum) of 3.0 mm long sealed by a capsule of titanium of 0.8 mm outside diameter, 0.05 mm wall thickness and 4,5mm long. This work aims to study and develop a seed of iridium-192 from a platinum-iridium alloy. No study on the fabrication of these seeds was found in available literature. It was created a methodology that involved: characterization of the material used in the core, creation of device for neutron activation irradiation and and seed sealing tests. As a result, proved the feasibility of the method. As a suggestion for future work, studies regarding metrology and dosimetry of these sources and improvement of the methodology should be carried out, for future implementation in national scope. (author)

  19. The genetic architecture of fitness in a seed beetle: assessing the potential for indirect genetic benefits of female choice

    DEFF Research Database (Denmark)

    Bilde, T.; Friberg, U.; Maklakov, A.A.; Fry, J.D.; Arnqvist, G.

    2008-01-01

    selection. In particular, the evolution of adaptive female choice by indirect genetic benefits relies on the presence of genetic variation for fitness. Female choice by genetic benefits fall broadly into good genes (additive) models and compatibility (non-additive) models where the strength of selection is...... of inbred lines, we assayed egg production, egg-to-adult survival, and lifetime offspring production of the outbred F1 daughters (F1 productivity). Results We used the bio model to estimate six components of genetic and environmental variance in fitness. We found sizeable additive and non......-additive genetic variance in F1 productivity, but lower genetic variance in egg-to-adult survival, which was strongly influenced by maternal and paternal effects. Conclusion Our results show that, in order to gain a relevant understanding of the genetic architecture of fitness, measures of offspring fitness should...

  20. Pyrimidine nucleoside phosphorylation in developing seeds and germinating seedlings of wheat

    International Nuclear Information System (INIS)

    Uridine- and thymidine-phosphorylating enzymes were measured in developing and germinating seeds of Triticum aestivum v. Arthur and T. aestivum v. Lemhi. Because crude extracts were to be used in the developmental study, characteristics of unpurified nucleoside phosphotransferase (NPTase) were examined. In the developmental study with two varieties of wheat, NPTase activity was found to be very low in all of the true seed tissues during seed maturation. Uridine-phosphorylating activity was due to primarily to uridine kinase. Thymidine phosphorylation was very low in all tissues throughout seed maturation, with a brief appearance by thymidine kinase in the developing embryo. In germinating seeds, uridine-phosphorylating activity was present from earliest stages of germination but showed a decrease in activity followed by a recovery after 48 hours inbibition. Experiments using [α-32P]ATP indicated that uridine kinase was present during early germination but had disappeared by 96 hours. Uridine phosphorylation at later stages of germination was accomplished by NTPase. Thymidine phosphorylation did not begin until after 36 hours of germination and was the result of NPTase activity

  1. GERMINATION PERFORMANCE OF SEQUENTIALLY HARVESTED TOMATO (LYCOPERSICON ESCULENTUM MILL.) SEED LOTS DURING SEED DEVELOPMENT UNDER SALT AND OSMOTIC STRESS

    OpenAIRE

    OZCOBAN, Mustafa; Demir, Ibrahim

    2006-01-01

    This work concluded that tomato seeds harvested 70 days after anthesis showed the maximum germination not only under water but also salt stress. Seeds harvested earlier or later were more sensitive to stress at germinations. High Ki value of the lots indicated high resistance under stress conditions.

  2. Biosynthesis of raffinose family oligosaccharides and galactosyl pinitols in developing and maturing seeds of winter vetch (Vicia vlllosa Roth.)

    OpenAIRE

    Lesław B. Lahuta

    2011-01-01

    Changes in the accumulation of two types of α-D-galactosides: raffinose family oligosaccharides and galactosyl pinitols were compared with changes in the activities of galactosyltransferases during winter vetch (Vicia villosa Roth.) seed development and maturation. Occurrence of galactinol and raffinose in young seeds and changes in activities of galactinol synthase and raffinose synthase during seed development indicated that formation of raffinose oligosaccharides (RFOs) preceded synthesis ...

  3. Developing a New Framework for Integration and Teaching of Computer Aided Architectural Design (CAAD) in Nigerian Schools of Architecture

    Science.gov (United States)

    Uwakonye, Obioha; Alagbe, Oluwole; Oluwatayo, Adedapo; Alagbe, Taiye; Alalade, Gbenga

    2015-01-01

    As a result of globalization of digital technology, intellectual discourse on what constitutes the basic body of architectural knowledge to be imparted to future professionals has been on the increase. This digital revolution has brought to the fore the need to review the already overloaded architectural education curriculum of Nigerian schools of…

  4. Development of esophageal 125I seed irradiated stent and its experiment

    International Nuclear Information System (INIS)

    Objective: To investigate the feasibility of developing the esophageal irradiating esophageal stent by placing 125I seed into it, the safety of the irradiative stent esophageal in animal experiments. Methods: (1) Development of esophageal 125I seed irradiated stent: a plastic 'bullet chamber' was moulded which held only one 125I seed. Its distribution and the spot where it was stitched on outside the stents. Thus esophageal intraluminal irradiating stents were developed. (2) Animal experiment: 36 rabbits were randomly assigned to 4 groups according to different irradiated dosage, 9 rabbits in each group. Intraluminal irradiated stents each with two 125I seeds totaling 22.2 MBq (group A), 44.4 MBq (group B) and 66.6 MBq (group C) respectively were placed in the rabbits esophagus and stents without 125I seeds were placed in group D rabbits esophagus which as control group. Three rabbits of each group were killed at the 2th, 4th, or 8th week after insertion of the stents, followed by esophageal contrast exam and CT scan to observe displacement of stents and transposition of 125I seed. The damage and its degree of esophagus and the lung, bronchus and large vessels around it caused by intraluminal irradiated stents were observed macroscopically or under optical and electric microscope. Results: (1) Development of esophageal intraluminal irradiated stents: 125I seed could be stitched on outside the stents firmly. Experiments of filling and releasing seeds in vitro were feasible. Irradiated steats could be easily placed in rabbits esophagus. It was safe for no losing of radioactive source has been found after they have been placed in esophagus for a long-term. (2) Animal experiments: the esophageal wall near 125I seed thickened most distinctly in mental groups during the 8th week[ group A(4.2 ± 0.3)mm,group B(4.3 ± 0.3)mm, group C(4.5 ± 0.3)mm]. There was a significant difference between the experimental group and control group [(3.1 ± 0.1) mm (P0.05). Four weeks

  5. Development of an Iridium-192 seed for use in ophthalmic brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Mattos, Fabio R.; Rostelato, Maria Elisa C.M.; Zeituni, Carlos; Moura, Joao A.; Costa, Osvaldo L.; Feher, Anselmo; Moura, Eduardo S.; Souza, Carla D.; Peleias Junior, Fernando S., E-mail: frmattos@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    The Institute for Energy and Nuclear Research (IPEN), in partnership with the School or Medicine (UNIFESP), created a project that aims to develop and implement an ophthalmic therapeutic treatment for cancer with Iridium-192 seeds. The School of Medicine treats many cancer cases in the SUS (Brazilian Public Health System), and brachytherapy group of IPEN has extensive experience in prototype sources. The seed to be manufactured will perform as follows: a core of Iridium-192 is packaged inside small cylindrical seeds consist of a titanium capsule of 0.8 mm outer diameter, 0.05 mm wall thickness and 4 5 mm in length. The core is an alloy of platinum-iridium (20/80) of 3.0 mm in length and 0.3 mm in diameter. Material analysis, neutron activation and activity measurements were carried out. (author)

  6. Development of a PC-based Open Architecture Software-CNC System

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    As a key technology in the field of advanced manufacturing, an open architecture controller is studied. In order to develop an open architecture software-CNC system on personal computer (PC) according to open modular architecture controllers (OMAC). First, the software and hardware platform is chosen and software realization methodology for the CNC system is determined. Second, static modeling methods of an open architecture controller inclusive of object-oriented (OO) programming technology, dynamic link library (DLL)technology and system modules partition are investigated. Third, the dynamical behavioral modeling and the data flow representation of open architecture controller are discussed, which are both described in hierarchy finite state machine (FSM) model. Fourth, a reusable software module model is established to develop software function module library. Finally, a 3-axis milling machine tool test-bed, named for HIT-CNC, is successfully designed by means of the constructed software function module library and the system configuring method.The experimental results show that, besides increasing the degree of reusability and openness, application of above-mentioned methodology leads to significant decrease of development time as well as maintenance cost.

  7. Autism Spectrum Disorder Symptoms among Children Enrolled in the Study to Explore Early Development (SEED)

    Science.gov (United States)

    Wiggins, Lisa D.; Levy, Susan E.; Daniels, Julie; Schieve, Laura; Croen, Lisa A.; DiGuiseppi, Carolyn; Blaskey, Lisa; Giarelli, Ellen; Lee, Li-Ching; Pinto-Martin, Jennifer; Reynolds, Ann; Rice, Catherine; Rosenberg, Cordelia Robinson; Thompson, Patrick; Yeargin-Allsopp, Marshalyn; Young, Lisa; Schendel, Diana

    2015-01-01

    This study examined the phenotypic profiles of children aged 30-68 months in the Study to Explore Early Development (SEED). Children classified as autism spectrum disorder (ASD), developmental delay (DD) with ASD symptoms, DD without ASD symptoms, and population comparison (POP) differed significantly from each other on cognitive, adaptive,…

  8. Vitamin E homologs and ¿-oryzanol levels in rice (Oryza sativa L.) during seed development

    Science.gov (United States)

    Vitamin E homologs (tocopherols and tocotrienols) and gamma-oryzanol have gained significant attention due to their proposed health benefits and ability to increase vegetable oil stability. Changes in the levels of these phytochemicals were examined during seed development. Rapid accumulation of toc...

  9. Accelerating Seed Germination and seedling development of Sorghum (Sorghum bicolor L. Moench) through hydro-priming

    Science.gov (United States)

    Dembele, S., Jr.

    2015-12-01

    Mali, a West Africa Sahelian country, is characterized by a strong dependence on rain-fed agriculture and a low adaptive capacity, making it one of the most vulnerable regions to climate change worldwide. Moreover, although with high uncertainties, most climate models used for the region recognize a growing uncertainty in the onset of the rainy season, which demands urgent adaptation measures. Early-season drought limits crops germination, and hence growth, and yield during rainfed depending production as is common now in Mali, West Africa. Crops germination and establishment could be improved by using seed priming, a process that dry seeds take up water to initiate the primary stages of germination, but the amount of water added is not enough for completing germination. The effects of hydro-priming (distilled, tap, rain, river and well water) were evaluated for three priming durations (4, 8 and 12 hour) in 2014 and 2015. Monitored were seed germination and seedling development of nine sorghum genotypes. Preliminary results showed that hydro-priming significantly improved germination rate, germination speed, number of seminal root, rate of survival and seedling vigour index, compared to non-primed seed treatments. However, seedling length, root length, shoot length and seedling dry weight did not differ significantly. Four out of the nine genotypes evaluated were attributed good seed quality and good response to hydro-priming. The priming with different sources of water resulted in higher seed germination (90%) and seedling development with well and river water, compared to the others. Seed germination rate, uniformity and speed were also enhanced by hydro-priming. It is argued that hydro-priming is a simple but effective method for improving seed germination and seedling development of sorghum. In addition hydro-priming is a safe, simple and inexpensive method to enhance germination. The most promising genotypes have consequently been included in consequent pot

  10. Development of Network-based Communications Architectures for Future NASA Missions

    Science.gov (United States)

    Slywczak, Richard A.

    2007-01-01

    Since the Vision for Space Exploration (VSE) announcement, NASA has been developing a communications infrastructure that combines existing terrestrial techniques with newer concepts and capabilities. The overall goal is to develop a flexible, modular, and extensible architecture that leverages and enhances terrestrial networking technologies that can either be directly applied or modified for the space regime. In addition, where existing technologies leaves gaps, new technologies must be developed. An example includes dynamic routing that accounts for constrained power and bandwidth environments. Using these enhanced technologies, NASA can develop nodes that provide characteristics, such as routing, store and forward, and access-on-demand capabilities. But with the development of the new infrastructure, challenges and obstacles will arise. The current communications infrastructure has been developed on a mission-by-mission basis rather than an end-to-end approach; this has led to a greater ground infrastructure, but has not encouraged communications between space-based assets. This alone provides one of the key challenges that NASA must encounter. With the development of the new Crew Exploration Vehicle (CEV), NASA has the opportunity to provide an integration path for the new vehicles and provide standards for their development. Some of the newer capabilities these vehicles could include are routing, security, and Software Defined Radios (SDRs). To meet these needs, the NASA/Glenn Research Center s (GRC) Network Emulation Laboratory (NEL) has been using both simulation and emulation to study and evaluate these architectures. These techniques provide options to NASA that directly impact architecture development. This paper identifies components of the infrastructure that play a pivotal role in the new NASA architecture, develops a scheme using simulation and emulation for testing these architectures and demonstrates how NASA can strengthen the new infrastructure by

  11. A COMPUTER APPLICATION FOR THE ARCHITECTURAL PROGRAM DEVELOPMENT IN DESIGN EDUCATION

    Directory of Open Access Journals (Sweden)

    Daniel de Carvalho Moreira

    2012-02-01

    Full Text Available The development of the architectural program in the design studio faces several difficulties. The purpose of the program is to describe the conditions where the building being designed will operate; this requires a lot of information and organization. Due to its complexity, the architetural program definition in the disciplines of design is often simplified. This article discusses such issue and proposes a computer application (SINFORMA that gathers information about the building and the theme of the project in order to develop the architectural program based on structures proposed by bibliographic references. The SINFORMA is composed by a framework which includes a data base and modules which analyze and organize functional requirements, according to the Problem Seeking method and the contemporary values of architecture enumerated by Hershberger. It is discussed how the application can be applied in design education and how it offers students a practical approach and a comprehensive data analysis for the design of built environment. Keywords: Architectural programming, Architectural design, Education.

  12. Exploration Space Suit Architecture and Destination Environmental-Based Technology Development

    Science.gov (United States)

    Hill, Terry R.; McFarland, Shane M.; Korona, F. Adam

    2013-01-01

    This paper continues forward where EVA Space Suit Architecture: Low Earth Orbit Vs. Moon Vs. Mars left off in the development of a space suit architecture that is modular in design and could be reconfigured prior to launch or during any given mission depending on the tasks or destination. This space suit system architecture and technologies required based on human exploration (EVA) destinations will be discussed, and how these systems should evolve to meet the future exploration EVA needs of the US human space flight program. A series of exercises and analyses provided a strong indication that the Constellation Program space suit architecture, with its maximum reuse of technology and functionality across a range of mission profiles and destinations, is postured to provide a viable solution for future space exploration missions. The destination environmental analysis demonstrates that the modular architecture approach could provide the lowest mass and mission cost for the protection of the crew, given any human mission outside of low-Earth orbit. Additionally, some of the high-level trades presented here provide a review of the environmental and nonenvironmental design drivers that will become increasingly important as humans venture farther from Earth. The presentation of destination environmental data demonstrates a logical clustering of destination design environments that allows a focused approach to technology prioritization, development, and design that will maximize the return on investment, largely independent of any particular design reference mission.

  13. Development of advanced nodal diffusion methods for modern computer architectures

    International Nuclear Information System (INIS)

    A family of highly efficient multidimensional multigroup advanced neutron-diffusion nodal methods, ILLICO, were implemented on sequential, vector, and vector-concurrent computers. Three-dimensional realistic benchmark problems can be solved in vectorized mode in less than 0.73 s (33.86 Mflops) on a Cray X-MP/48. Vector-concurrent implementations yield speedups as high as 9.19 on an Alliant FX/8. These results show that the ILLICO method preserves essentially all of its speed advantage over finite-difference methods. A self-consistent higher-order nodal diffusion method was developed and implemented. Nodal methods for global nuclear reactor multigroup diffusion calculations which account explicitly for heterogeneities in the assembly nuclear properties were developed and evaluated. A systematic analysis of the zero-order variable cross section nodal method was conducted. Analyzing the KWU PWR depletion benchmark problem, it is shown that when burnup heterogeneities arise, ordinary nodal methods, which do not explicitly treat the heterogeneities, suffer a significant systematic error that accumulates. A nodal method that treats explicitly the space dependence of diffusion coefficients was developed and implemented. A consistent burnup-correction method for nodal microscopic depletion analysis was developed

  14. Exploring Sustainable Industrial Software System Development : within the Software Architecture Environment

    OpenAIRE

    Stoll, Pia

    2009-01-01

    This thesis describes how sustainable development definitions can be transposed to the software architecture environment for the industrial software system domain. In a case study, sustainable development concerns from three companies are investigated for their influence on the dimensions of sustainable development: economical, environmental, and social sustainability. Classifying the case study’s concerns, in the thesis’s Software Engineering taxonomy, shows that the software development con...

  15. Groupware architecture for requirements processes in new product development

    OpenAIRE

    Salo, Ahti; Käkölä, Timo

    1999-01-01

    An organization's ability to create successful products depends on how extensively it collects, analyzes and utilizes requirements information in its product development. Recent advances in groupware technologies allow such information to be shared more effectively in distributed organizations; thus, these technologies hold considerable potential as means of shortening the cycle time and improving the quality of requirements processes. This paper addresses issues in the design and implementat...

  16. On the Design of Next Generation Network Architecture for a Developing Economy

    Directory of Open Access Journals (Sweden)

    Nathaniel S. Tarkaa

    2016-08-01

    Full Text Available The design of network architecture is an essential step in the design of Next Generation Networks, leading to the prediction of the actual equipment quantities and dimensioning of the network. The network architecture is commonly drawn out as a diagram for visual representation of the overall network. This paper focuses on the design of the architectural model for a softswitch IP based backbone network for Benue State, Nigeria, as a case study of developing economies. It has been found that, in developing countries, the migration from the Public Switched Telephone Network to Next Generation Network takes the form of a major telecommunications development scheme meant for the extension of telecommunications infrastructure and services to new deserved areas and/or the modernization of the predominantly obsolete and substandard existing telecommunications infrastructure. So, the basic network planning principles have been followed to design the architecture in the most efficient and cost-effective manner possible. The key design methodology is based on intuitive demand forecasting, a snapshot of the existing network infrastructure and systematic assessment of the Public Switched Telephone Network-to-Next Generation Network migration principles. It was furthermore guided by use of the ITU-T’s generic reference models enabling the proper combinations of the functional entities. The resulting Next Generation Network backbone architecture is made up of 2 Softswitches, 23 Universal Media Gateways and an MPLS/IP core. The further optimization of the architecture, which is normally achieved with dimensioning of the network, is beyond the scope of this work.

  17. Oxygen control of ethylene biosynthesis during seed development in Arabidopsis thaliana (L.) Heynh

    Science.gov (United States)

    Ramonell, K. M.; McClure, G.; Musgrave, M. E.

    2002-01-01

    An unforeseen side-effect on plant growth in reduced oxygen is the loss of seed production at concentrations around 25% atmospheric (50 mmol mol-1 O2). In this study, the model plant Arabidopsis thaliana (L.) Heynh. cv. 'Columbia' was used to investigate the effect of low oxygen on ethylene biosynthesis during seed development. Plants were grown in a range of oxygen concentrations (210 [equal to ambient], 160, 100, 50 and 25 mmol mol-1) with 0.35 mmol mol-1 CO2 in N2. Ethylene in full-sized siliques was sampled using gas chromatography, and viable seed production was determined at maturity. Molecular analysis of ethylene biosynthesis was accomplished using cDNAs encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase in ribonuclease protection assays and in situ hybridizations. No ethylene was detected in siliques from plants grown at 50 and 25 mmol mol-1 O2. At the same time, silique ACC oxidase mRNA increased three-fold comparing plants grown under the lowest oxygen with ambient controls, whereas ACC synthase mRNA was unaffected. As O2 decreased, tissue-specific patterning of ACC oxidase and ACC synthase gene expression shifted from the embryo to the silique wall. These data demonstrate how low O2 modulates the activity and expression of the ethylene biosynthetic pathway during seed development in Arabidopsis.

  18. How the Timing and Quality of Early Experiences Influence the Development of Brain Architecture

    Science.gov (United States)

    Fox, Sharon E.; Levitt, Pat; Nelson, Charles A., III.

    2010-01-01

    Early life events can exert a powerful influence on both the pattern of brain architecture and behavioral development. In this study a conceptual framework is provided for considering how the structure of early experience gets "under the skin." The study begins with a description of the genetic framework that lays the foundation for brain…

  19. Exploring Architectures for Fast and Easy Development of Immersive Learning Scenarios

    NARCIS (Netherlands)

    Nadolski, Rob; Slootmaker, Aad; Hummel, Hans

    2009-01-01

    Nadolski, R. J., Slootmaker, A., & Hummel, H. G. K. (2009). Exploring Architectures for Fast and Easy Development of Immersive Learning Scenarios. Presentation given at Online Educa. December, 3-4, 2009, Berlin, Germany. [For Powerpoint-version of this presentation. Please see http://www.slideshare.

  20. Business Architecture Development at Public Administration - Insights from Government EA Method Engineering Project in Finland

    Science.gov (United States)

    Valtonen, Katariina; Leppänen, Mauri

    Governments worldwide are concerned for efficient production of services to customers. To improve quality of services and to make service production more efficient, information and communication technology (ICT) is largely exploited in public administration (PA). Succeeding in this exploitation calls for large-scale planning which embraces issues from strategic to technological level. In this planning the notion of enterprise architecture (EA) is commonly applied. One of the sub-architectures of EA is business architecture (BA). BA planning is challenging in PA due to a large number of stakeholders, a wide set of customers, and solid and hierarchical structures of organizations. To support EA planning in Finland, a project to engineer a government EA (GEA) method was launched. In this chapter, we analyze the discussions and outputs of the project workshops and reflect emerged issues on current e-government literature. We bring forth insights into and suggestions for government BA and its development.

  1. Importance of rice research and development in rice seed policies: Insights from Nigeria:

    OpenAIRE

    Takeshima, Hiroyuki

    2014-01-01

    First, this paper shows that rice varietal development in Nigeria has been lagging behind that of other developing countries in Asia and Latin America, due partly to insufficient investment in domestic rice R&D. The paper then illustrates using a household model simulation that impacts of certain policies, such as the seed subsidy, may be greater (smaller) if they are applied to good (poor) varieties. The paper concludes by discussing key policy implications and future research needs.

  2. Towards a Reference Architecture to Provision Tools as a Service for Global Software Development

    DEFF Research Database (Denmark)

    Chauhan, Aufeef; Babar, Muhammad Ali

    2014-01-01

    Organizations involve in Global Software Development (GSD) face challenges in terms of having access to appropriate set of tools for performing distributed engineering and development activities, integration between heterogeneous desktop and web-based tools, management of artifacts developed and...... distributed environment. In this paper, we argue the need to have a cloud-enabled platform for supporting GSD and propose reference architecture of a cloud based Platform for providing support to provision ecosystem of the Tools as a Service (PTaaS)....

  3. ARCHITECTURAL EDUCATION AND SCHOOLING: BETWEEN THE SUSTAINABLE DEVELOPMENT AND PERCEPTION OF THE ENVIRONMENT

    OpenAIRE

    Beatriz Tomšič Čerkez

    2014-01-01

    If we consider the role of education and its implications in the formation of a critical and conscious user of architecture, it is obvious that the development of educational strategies related to our common space and environment become fundamental. The comprehension of the concept of sustainable development is essential. Therefore, it is worth reflecting on how to develop proper programs at all educational levels to promote critical and responsible attitudes towards the common environment co...

  4. Certain physiological, biochemical and molecular aspects of kidney bean plants originating from gamma-irradiated seeds during seed germination and plant development

    International Nuclear Information System (INIS)

    Dry seeds of kidney bean (phaseolus vulgaris cv. Giza 6) were irradiated with different dosages of gamma rays (2.5-15.0 k.rad) and germinated under laboratory conditions or sown in soil. The produced seedlings and growing plants at different stages of development were analysed for some physiological and biochemical events. In addition, SDS-PAGE profile of the harvested seeds was investigated. Results obtained revealed that the germination potential of kidney bean seeds and growth criteria of the produced seedlings were mostly highly significantly increased due to irradiating the seeds with the relatively low dosages of gamma rays (2.5 and 5.0 K. rad). The contents of DNA, RNA, protein-N, total-N, poly-saccharides and total sugars were substantially increased. This was at the expense of amino-N, glucose and sucrose contents coupled with a reduced leakage of the soluble metabolites from the seedlings. The growth parameters and yield components of plants exhibited mostly high significant increases with concomitant marked increases in the contents of auxins (IAA), gibberellins (GA3)and cytokinins (BA). On the other hand, all the aforementioned indices underwent a reverse pattern of change as a consequence of irradiating the seeds with 10.0 and 15.0 K.rad. SDS-PAGE profile of proteins extracted from the seeds harvested from plants originated from the seeds irradiated with 2.5 or 150.0 K.rad exhibited a disappearance of one protein band(M.W: 69.83 kDa) or four ones (M.W:69.83, 61.14, 51.29 and 46.86 kDa), respectively. The total number of protein bands remained unchanged in case of 5.0 and 10.0 k.rad exposure. Moreover, the intensities of protein in the existed bands showed variable changes

  5. Software architecture

    CERN Document Server

    Vogel, Oliver; Chughtai, Arif

    2011-01-01

    As a software architect you work in a wide-ranging and dynamic environment. You have to understand the needs of your customer, design architectures that satisfy both functional and non-functional requirements, and lead development teams in implementing the architecture. And it is an environment that is constantly changing: trends such as cloud computing, service orientation, and model-driven procedures open up new architectural possibilities. This book will help you to develop a holistic architectural awareness and knowledge base that extends beyond concrete methods, techniques, and technologi

  6. Soybean roots retain the seed urease isozyme synthesized during embryo development

    International Nuclear Information System (INIS)

    Roots of young soybean plants contain two urease isozymes which are separable by hydroxyapatite chromatography. These two urease species (HAP1 and HAP2) differ in: (1) native gel electrophoretic mobility, (2) pH optima, and (3) recognition by a monoclonal antibody specific for the embryo-specific urease. By these parameters HAP1 is similar to the abundant embryo-specific urease isozyme while HAP2 resembles the ubiquitous urease, found in all soybean tissues previously examined (embryo, seed coat, cultured cells). Roots of mutant soybean plants lacking the seed urease contain no HAP1 urease activity, whereas roots of mutants lacking the ubiquitous urease contain no HAP2 urease activity. However, adventitious roots generated from cuttings of any urease genotype lack HAP1 urease activity. Furthermore, [35S] methionine labelling shows no de novo synthesis of the HAP1 urease in the root, and total root HAP1 urease activity decreases sharply following germination. We conclude: (1) HAP1 is a remnant of the seed urease accumulated in the embryonic root axis during seed development, and (2) HAP2 is ubiquitous urease synthesized de novo in the root

  7. Seed treatments enhance photosynthesis in maize seedlings by reducing infection with Fusarium spp. and consequent disease development in maize

    Science.gov (United States)

    The effects of a seed treatment on early season growth, seedling disease development, incidence Fusarium spp. infection, and photosynthetic performance of maize were evaluated at two locations in Iowa in 2007. Maize seed was either treated with Cruiser 2Extreme 250 ® (fludioxonil + azoxystrobin + me...

  8. Metabolomic and Proteomic Profiles Reveal the Dynamics of Primary Metabolism during Seed Development of Lotus (Nelumbo nucifera).

    Science.gov (United States)

    Wang, Lei; Fu, Jinlei; Li, Ming; Fragner, Lena; Weckwerth, Wolfram; Yang, Pingfang

    2016-01-01

    Sacred lotus (Nelumbo nucifera) belongs to the Nelumbonaceae family. Its seeds are widely consumed in Asian countries as snacks or even medicine. Besides the market value, lotus seed also plays a crucial role in the lotus life cycle. Consequently, it is essential to gain a comprehensive understanding of the development of lotus seed. During its development, lotus seed undergoes cell division, expansion, reserve accumulation, desiccation, and maturation phases. We observed morphological and biochemical changes from 10 to 25 days after pollination (DAP) which corresponded to the reserve synthesis and accumulation phase. The volume of the seed expanded until 20 DAP with the color of the seed coat changing from yellow-green to dark green and gradually fading again. Starch and protein rapidly accumulated from 15 to 20 DAP. To further reveal metabolic adaptation, primary metabolites and proteins profiles were obtained using mass spectrometry based platforms. Metabolites and enzymes involved in sugar metabolism, glycolysis, TCA cycle and amino acid metabolism showed sequential dynamics enabling the clear separation of the different metabolic states during lotus seed development. The integration of the data revealed a highly significant metabolic switch at 15 DAP going through a transition of metabolically highly active tissue to the preparation of storage tissue. The results provide a reference data set for the evaluation of primary metabolism during lotus seed development. PMID:27375629

  9. Architecture Sustainability

    OpenAIRE

    Avgeriou, Paris; Stal, Michael; Hilliard, Rich

    2013-01-01

    Software architecture is the foundation of software system development, encompassing a system's architects' and stakeholders' strategic decisions. A special issue of IEEE Software is intended to raise awareness of architecture sustainability issues and increase interest and work in the area. The first Web extra at http://youtu.be/wUGHvocfix0 is an audio interview in which Davide Falessi speaks with guest editors Paris Avgeriou and Rich Hilliard about the importance of architecture sustainabil...

  10. Rapid development of adaptive, climate-driven clinal variation in seed mass in the invasive annual Forb Echium plantagineum L.

    Science.gov (United States)

    Konarzewski, Tara K; Murray, Brad R; Godfree, Robert C

    2012-01-01

    We examined adaptive clinal variation in seed mass among populations of an invasive annual species, Echium plantagineum, in response to climatic selection. We collected seeds from 34 field populations from a 1,000 km long temperature and rainfall gradient across the species' introduced range in south-eastern Australia. Seeds were germinated, grown to reproductive age under common glasshouse conditions, and progeny seeds were harvested and weighed. Analyses showed that seed mass was significantly related to climatic factors, with populations sourced from hotter, more arid sites producing heavier seeds than populations from cooler and wetter sites. Seed mass was not related to edaphic factors. We also found that seed mass was significantly related to both longitude and latitude with each degree of longitude west and latitude north increasing seed mass by around 2.5% and 4% on average. There was little evidence that within-population or between-population variation in seed mass varied in a systematic manner across the study region. Our findings provide compelling evidence for development of a strong cline in seed mass across the geographic range of a widespread and highly successful invasive annual forb. Since large seed mass is known to provide reproductive assurance for plants in arid environments, our results support the hypothesis that the fitness and range potential of invasive species can increase as a result of genetic divergence of populations along broad climatic gradients. In E. plantagineum population-level differentiation has occurred in 150 years or less, indicating that the adaptation process can be rapid. PMID:23284621

  11. Rapid development of adaptive, climate-driven clinal variation in seed mass in the invasive annual Forb Echium plantagineum L.

    Directory of Open Access Journals (Sweden)

    Tara K Konarzewski

    Full Text Available We examined adaptive clinal variation in seed mass among populations of an invasive annual species, Echium plantagineum, in response to climatic selection. We collected seeds from 34 field populations from a 1,000 km long temperature and rainfall gradient across the species' introduced range in south-eastern Australia. Seeds were germinated, grown to reproductive age under common glasshouse conditions, and progeny seeds were harvested and weighed. Analyses showed that seed mass was significantly related to climatic factors, with populations sourced from hotter, more arid sites producing heavier seeds than populations from cooler and wetter sites. Seed mass was not related to edaphic factors. We also found that seed mass was significantly related to both longitude and latitude with each degree of longitude west and latitude north increasing seed mass by around 2.5% and 4% on average. There was little evidence that within-population or between-population variation in seed mass varied in a systematic manner across the study region. Our findings provide compelling evidence for development of a strong cline in seed mass across the geographic range of a widespread and highly successful invasive annual forb. Since large seed mass is known to provide reproductive assurance for plants in arid environments, our results support the hypothesis that the fitness and range potential of invasive species can increase as a result of genetic divergence of populations along broad climatic gradients. In E. plantagineum population-level differentiation has occurred in 150 years or less, indicating that the adaptation process can be rapid.

  12. Spatial organisation and biomass development after relaying of mussel seed

    Science.gov (United States)

    Capelle, Jacob J.; Wijsman, Jeroen W. M.; Schellekens, Tim; van Stralen, Marnix R.; Herman, Peter M. J.; Smaal, Aad C.

    2014-01-01

    It is not known whether and by what factors spatial heterogeneity in mussels (Mytilus edulis L.) affects mussel production in human-created mussel beds. In a field experiment, the same number of mussels was relayed on four different areas within plots of the same size, resulting in four treatments with different mussel densities. Density, individual weight and spatial structure of mussels were followed per treatment. The uniformly placed mussels on different areas redistributed into new patches, but mussels did not spread out over a larger area. Initial mussel density affected redistribution and mussel survival. At high densities mussels redistributed into a uniform matrix or in a few larger patches, that showed larger losses than at low densities, where mussels redistributed into a high number of patches. Growth rate and condition index of the mussels did not differ between treatments and no relation was found between treatment and number of foraging shore crabs, which was the major predator of mussels in this experiment. We hypothesise that the relation between initial mussel density and mussel loss after relaying is associated with redistribution, with less competition for space when mussels are positioned at the edge of a mussel patch. The very high mussel losses that we observed in the experiment within four weeks after relaying were the major factor in biomass development. Mussel bed formation concerns mussel growers and managers involved in natural mussel bed restoration. Initial mussel survival determines the success of these activities. The present study shows the effects of mussel relaying on spatial redistribution for the first time under field conditions, and underlines the importance of edge effects in understanding mussel loss in redistribution. Mussel survival after relaying will be higher when the mussels are distributed homogeneously and in relatively low density.

  13. Development of an encapsulation method using plasma arc welding to produce iodine-125 seeds for brachytherapy

    International Nuclear Information System (INIS)

    The prostate cancer, which is the second cause of death by cancer in men, overcome only by lung cancer is public health problem in Brazil. Brachytherapy is among the possible available treatments for prostate cancer, in which small seeds containing Iodine-125 radioisotope are implanted into the prostate gland. The seed consists of a titanium sealed capsule with 0.8 mm external diameter and 4.5 mm length, containing a central silver wire with adsorbed Iodine-125. The Plasma Arc Welding (PAW) is one of the viable techniques for sealing process. The equipment used in this technique is less costly than in other processes, such as, Laser Beam Welding (LBW). The main purpose of this work was the development of an encapsulation method using PAW. The development of this work has presented the following phases: cutting and cleaning titanium tube, determination of the welding parameters, development of a titanium tube holding device for PAW, sealed sources validation according to ISO 2919 - Sealed Radioactive Sources - General Requirements and Classification, and metallographic assays. The developed procedure to seal Iodine-125 seeds using PAW has shown high efficiency, satisfying all the established requirements of ISO 2919. The results obtained in this work will give the possibility to establish a routine production process according to the orientations presented in resolution RDC 17 - Good Manufacturing Practices to Medical Products defined by the ANVISA - National Agency of Sanitary Surveillance. (author)

  14. Development of an encapsulation method using plasma arc welding to produce iodine-125 seeds for brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Feher, Anselmo; Calvo, Wilson A.P.; Rostelato, Maria E.C.M.; Zeituni, Carlos A.; Somessari, Samir L.; Costa, Osvaldo L.; Moura, Joao A.; Moura, Eduardo S.; Souza, Carla D.; Rela, Paulo R., E-mail: afeher@ipen.b, E-mail: wapcalvo@ipen.b, E-mail: elisaros@ipen.b, E-mail: somessar@ipen.b, E-mail: olcosta@ipen.b, E-mail: esmoura@ipen.b, E-mail: cdsouza@ipen.b, E-mail: prela@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    The prostate cancer, which is the second cause of death by cancer in men, overcome only by lung cancer is public health problem in Brazil. Brachytherapy is among the possible available treatments for prostate cancer, in which small seeds containing Iodine-125 radioisotope are implanted into the prostate gland. The seed consists of a titanium sealed capsule with 0.8 mm external diameter and 4.5 mm length, containing a central silver wire with adsorbed Iodine-125. The Plasma Arc Welding (PAW) is one of the viable techniques for sealing process. The equipment used in this technique is less costly than in other processes, such as, Laser Beam Welding (LBW). The main purpose of this work was the development of an encapsulation method using PAW. The development of this work has presented the following phases: cutting and cleaning titanium tube, determination of the welding parameters, development of a titanium tube holding device for PAW, sealed sources validation according to ISO 2919 - Sealed Radioactive Sources - General Requirements and Classification, and metallographic assays. The developed procedure to seal Iodine-125 seeds using PAW has shown high efficiency, satisfying all the established requirements of ISO 2919. The results obtained in this work will give the possibility to establish a routine production process according to the orientations presented in resolution RDC 17 - Good Manufacturing Practices to Medical Products defined by the ANVISA - National Agency of Sanitary Surveillance. (author)

  15. Collaborative Decision Making: Complementary Developments of a Model and an Architecture as a Tool Support

    OpenAIRE

    Jankovic, Marija; ZARATÉ, Pascale; Bocquet, Jean-Claude; Stal-Le Cardinal, Julie

    2009-01-01

    Cardinal (2009). "Collaborative Decision Making: Complementary Developments of a Model and an Architecture as a Tool Support." International Journal of Decision Support System Technology (IJDSST) 1(1): 35-45. ABSTRACT. Recent years we can hear a lot about cooperative decision-making, group or collaborative decision-making. These types of decisions are the consequences of developed working conditions: geographical dispersion, team working, and concurrent working. In the paper we present two re...

  16. Boosting Seed Development as a New Strategy to Increase Cotton Fiber Yield and Quality

    Institute of Scientific and Technical Information of China (English)

    Yong-Ling Ruan

    2013-01-01

    Cotton (Gossypium spp.) is the most important textile crop worldwide due to its cellulosic mature fibers,which are single-celled hairs initiated from the cotton ovule epidermis at anthesis.Research to improve cotton fiber yield and quality in recent years has been largely focused on identifying genes regulating fiber cell initiation,elongation and cellulose synthesis.However,manipulating some of those candidate genes has yielded no effect or only a marginally positive effect on fiber yield or quality.On the other hand,evolutionary comparison and transgenic studies have clearly shown that cotton fiber growth is intimately controlled by seed development.Therefore,I propose that enhancing seed development could be a more effective and achievable strategy to increase fiber yield and quality.

  17. The seed coat of Phaseolus vulgaris interferes with the development of the cowpea weevil [Callosobruchus maculatus (F. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Silva Luciana B.

    2004-01-01

    Full Text Available We have confirmed here that the seeds of the common bean (Phaseolus vulgaris, L. do not support development of the bruchid Callosobruchus maculatus (F., a pest of cowpea [Vigna unguiculata (L. Walp] seeds. Analysis of the testa (seed coat of the bean suggested that neither thickness nor the levels of compounds such as tannic acid, tannins, or HCN are important for the resistance. On the other hand, we have found that phaseolin (vicilin-like 7S storage globulin, detected in the testa by Western blotting and N-terminal amino acid sequencing, is detrimental to the development of C. maculatus. As for the case of other previously studied legume seeds (Canavalia ensiformis and Phaseolus lunatus we suggest that the presence of vicilin-like proteins in the testa of P. vulgaris may have had a significant role in the evolutionary adaptation of bruchids to the seeds of leguminous plants.

  18. Control of Seed Germination and Plant Development by Carbon and Nitrogen Availability

    OpenAIRE

    Osuna, Daniel; Prieto, Pilar; Aguilar, Miguel

    2015-01-01

    Little is known about the molecular basis of the influence of external carbon/nitrogen (C/N) ratio and other abiotic factors on phytohormones regulation during seed germination and plant developmental processes, and the identification of elements that participate in this response is essential to understand plant nutrient perception and signaling. Sugars (sucrose, glucose) and nitrate not only act as nutrients but also as signaling molecules in plant development. A connection between changes i...

  19. Autism Spectrum Disorder Symptoms Among Children Enrolled in the Study to Explore Early Development (SEED)

    OpenAIRE

    Wiggins, Lisa D.; Levy, Susan E.; Daniels, Julie; Schieve, Laura; Croen, Lisa A.; DiGuiseppi, Carolyn; Blaskey, Lisa; Giarelli, Ellen; Lee, Li-Ching; Pinto-Martin, Jennifer; Reynolds, Ann; Rice, Catherine; Rosenberg, Cordelia Robinson; Thompson, Patrick; Yeargin-Allsopp, Marshalyn

    2015-01-01

    This study examined the phenotypic profiles of children aged 30–68 months in the Study to Explore Early Development (SEED). Children classified as autism spectrum disorder (ASD), developmental delay (DD) with ASD symptoms, DD without ASD symptoms, and population comparison (POP) differed significantly from each other on cognitive, adaptive, behavioral, and social functioning and the presence of parent-reported conditions. Children with ASD and DD with ASD symptoms had mild to severe ASD risk ...

  20. Catalyst Architecture

    DEFF Research Database (Denmark)

    Kiib, Hans; Marling, Gitte; Hansen, Peter Mandal

    2014-01-01

    How can architecture promote the enriching experiences of the tolerant, the democratic, and the learning city - a city worth living in, worth supporting and worth investing in? Catalyst Architecture comprises architectural projects, which, by virtue of their location, context and their combination...... of programs, have a role in mediating positive social and/or cultural development. In this sense, we talk about architecture as a catalyst for: sustainable adaptation of the city’s infrastructure appropriate renovation of dilapidated urban districts strengthening of social cohesiveness in the city...

  1. Evaluation of germination, vegetative development and genotoxicity of lettuce from irradiated seeds

    Energy Technology Data Exchange (ETDEWEB)

    Franco, Caio H.; Arthur, Valter, E-mail: caiohaddadfranco@lnbio.cnpem.com.br, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Radiobiologia e Ambiente; Silva, Regildo M.G. da, E-mail: regildo@assis.unesp.br [Universidade Estadual Paulista Julio de Mesquita Filho (UNESP), Assis, SP (Brazil). Fac. de Ciencias de Letras. Lab. de Fitoterapicos e Farmacologia; Franco, Jose G.; Franco, Suely S. H., E-mail: gilmita@uol.com.br, E-mail: zegilmar60@gmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    Agriculture has benefited from the use of radiation techniques, which provides plant varieties with distinguish characteristics, such as higher productivity, precocity and greater resistance to disease, pests and harsh weather conditions. Therefore, this study aimed on the analysis of greenhouse morphological development of Lactuca sativa originated from irradiated seeds; as well as test their genotoxic effect. The seeds were irradiated at doses of 25, 50, 75, 150 and 300 Gy. In order to determine the germination index, the number of seedlings emerged from each well was counted. Biometric and weight measurements were taken during the development and post-harvest stages. Genotoxicity tests were performed based on the biological assay Allium cepa. The results demonstrated that the best vegetative development was observed for individuals originated from seeds irradiated with doses of 25 and 50 Gy when compared with the control, while this dose did not differ significantly from 75 Gy The calculated germination index remained constant at all dosages. Inhibition of vegetative growth was observed on 150 and 300 Gy dosed individuals. It was also observed that the increasing rate of irradiation is inversely proportional to the mitotic index. A relationship can be established between increased levels of irradiation with increasing percentage of aberrant cells. (author)

  2. Development and characterisation of iridium-192 seeds for brachytherapy treatment of ocular tumors

    International Nuclear Information System (INIS)

    Even ocular tumors are not amongst the cases with a high incidence, they affect the population, particularly children. The Institute of Energy and Nuclear Research (IPEN-CNEN/SP) in partnership with Escola Paulista de Medicina (UNIFESP), created a project to develop an alternative treatment for ophthalmic cancer that uses iridium-192 seeds in brachytherapy. This work aims to study and develop a seed of iridium-192 from a platinum-iridium alloy The prototype seed has a 3.0 mm long core sealed by a titanium capsule of 0.8 mm of outer diameter, 0.05 mm of wall thickness and 4.5 mm long. We developed a methodology that covered: characterisation of the material used in the core, creation of a device for neutron activation of the cores and leakage tests. The results show that this methodology is feasible. As a suggestion for future work, studies regarding metrology and dosimetry of these sources should be carried out. (authors)

  3. Genome-wide expression analysis of soybean MADS genes showing potential function in the seed development.

    Directory of Open Access Journals (Sweden)

    Cheng-Ming Fan

    Full Text Available The MADS family is an ancient and best-studied transcription factor and plays fundamental roles in almost every developmental process in plants. In the plant evolutionary history, the whole genome duplication (WGD events are important not only to the plant species evolution, but to expansion of members of the gene families. Soybean as a model legume crop has experience three rounds of WGD events. Members of some MIKC(C subfamilies, such as SOC, AGL6, SQUA, SVP, AGL17 and DEF/GLO, were expanded after soybean three rounds of WGD events. And some MIKC(C subfamilies, MIKC* and type I MADS families had experienced faster birth-and-death evolution and their traces before the Glycine WGD event were not found. Transposed duplication played important roles in tandem arrangements among the members of different subfamilies. According to the expression profiles of type I and MIKC paralog pair genes, the fates of MIKC paralog gene pairs were subfunctionalization, and the fates of type I MADS paralog gene pairs were nonfunctionalization. 137 out of 163 MADS genes were close to 186 loci within 2 Mb genomic regions associated with seed-relative QTLs, among which 115 genes expressed during the seed development. Although MIKC(C genes kept the important and conserved functions of the flower development, most MIKC(C genes showed potentially essential roles in the seed development as well as the type I MADS.

  4. Evaluation of germination, vegetative development and genotoxicity of lettuce from irradiated seeds

    International Nuclear Information System (INIS)

    Agriculture has benefited from the use of radiation techniques, which provides plant varieties with distinguish characteristics, such as higher productivity, precocity and greater resistance to disease, pests and harsh weather conditions. Therefore, this study aimed on the analysis of greenhouse morphological development of Lactuca sativa originated from irradiated seeds; as well as test their genotoxic effect. The seeds were irradiated at doses of 25, 50, 75, 150 and 300 Gy. In order to determine the germination index, the number of seedlings emerged from each well was counted. Biometric and weight measurements were taken during the development and post-harvest stages. Genotoxicity tests were performed based on the biological assay Allium cepa. The results demonstrated that the best vegetative development was observed for individuals originated from seeds irradiated with doses of 25 and 50 Gy when compared with the control, while this dose did not differ significantly from 75 Gy The calculated germination index remained constant at all dosages. Inhibition of vegetative growth was observed on 150 and 300 Gy dosed individuals. It was also observed that the increasing rate of irradiation is inversely proportional to the mitotic index. A relationship can be established between increased levels of irradiation with increasing percentage of aberrant cells. (author)

  5. Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds.

    Science.gov (United States)

    Gangola, Manu P; Jaiswal, Sarita; Kannan, Udhaya; Gaur, Pooran M; Båga, Monica; Chibbar, Ravindra N

    2016-05-01

    To understand raffinose family oligosaccharides (RFO) metabolism in chickpea (Cicer arietinum L.) seeds, RFO accumulation and corresponding biosynthetic enzymes activities were determined during seed development of chickpea genotypes with contrasting RFO concentrations. RFO concentration in mature seeds was found as a facilitator rather than a regulating step of seed germination. In mature seeds, raffinose concentrations ranged from 0.38 to 0.68 and 0.75 to 0.99 g/100 g, whereas stachyose concentrations varied from 0.79 to 1.26 and 1.70 to 1.87 g/100 g indicating significant differences between low and high RFO genotypes, respectively. Chickpea genotypes with high RFO concentration accumulated higher concentrations of myo-inositol and sucrose during early seed developmental stages suggesting that initial substrate concentrations may influence RFO concentration in mature seeds. High RFO genotypes showed about two to three-fold higher activity for all RFO biosynthetic enzymes compared to those with low RFO concentrations. RFO biosynthetic enzymes activities correspond with accumulation of individual RFO during seed development. PMID:26953100

  6. DEPENDABLE WEB SERVICES SECURITY ARCHITECTURE DEVELOPMENT THEORETICAL AND PRACTICAL ISSUES – SPATIAL WEB SERVICES CASE STUDY

    OpenAIRE

    D. Shravani; P. Suresh Varma; K.Venkateswar Rao; B.Padmaja Rani; M.Upendra Kumar

    2012-01-01

    This research “Designing Dependable Web Services Security Architecture Solutions” addresses the innovative idea of Web Services Security Engineering using Web Services Security Architecture with a research motivation of Secure Service Oriented Analysis and Design. It deals with Web Services Security Architecture for Web Services Secure application design, for Authentication and authorization, using Model Driven Architecture (MDA) based Agile Modeled Layered Security Architectur...

  7. DEVELOPMENT OF SET AND DRINKING SESAME YOGHURT FROM DECORTICATED SESAME SEED

    Directory of Open Access Journals (Sweden)

    Ibrahim Abdullah Afaneh

    2013-01-01

    Full Text Available Sesame yoghurt developed from sesame seed fortified with different fortifiers resulted in yoghurt that lacks the typical yogurt texture. This study aimed to develop set and drinking yoghurt from sesame milk. Gums were used to develop sesame set yoghurt and sesame yoghurt drink. The best set yoghurt was produced from the sesame milk fortified with 2% Cheese Dried Whey (CDW plus 0.6% pectin and 0.2% CMC. The best yoghurt drink was produced from sesame milk fortified with dried whey plus 0.2% guar gum and pasteurized at 75°C for 5 min.

  8. Development of an Automated Seed Sowing and Induced Germination System for Space Flight Application

    Science.gov (United States)

    Heyenga, A. G.; Kliss, Mark

    1995-01-01

    The successful utilization of higher plants in space flight is likely to require the effective transition of plants through all phases of growth and development. A particularly sensitive and critical stage in this cycle is seed germination. The present inflight capability to manipulate seed from a state of dormancy to germination and the performance of such activity under aseptic conditions is extremely limited. An Automated Sowing Mechanism (ASM) has been designed to address this area of science and technology. The self-contained system is readily compatible with the existing Shuttle middeck locker Plant Growth Unit (PGU) and planned Plant Growth Facility (PGF), presenting an opportunity to extend the experimental capability of these systems. The ASM design encompasses the controlled transition of seed from a dry to hydrated state utilizing solid media substrate as the source of water and nutrient support. System activation has been achieved with both photo and timing mechanisms. Controlled induced germination and development of various plant species has been achieved in ground-based trials. The system is presently being prepared for a KC-135 flight test.

  9. DEVELOPMENT AND RESEARCH MODELS OF BUSINESS ARCHITECTURE OF SUBSYSTEM SALES OF CORPORATE INTEGRATED STRUCTURE

    Directory of Open Access Journals (Sweden)

    Baranovskaya T. P.

    2015-10-01

    Full Text Available The article describes basic principles and the results of modeling the business architecture of the company on the example of the subsystem sales of integrated corporate structures. As the object of investigation we have chosen the company called Dinskoy Meatprocessing complex, which is part of Southern diversified Corporation. The main artifacts of the conducted research are: graphical layout of business processes of a subsystem of the sales made in the instrumental environment of All Fusion Process Modeler (BPWin and developed a model of decomposition of business processes, a model of business event, a model for the location of functions and integration model, and to evaluate the relationship between the business processes with the strategic direction of the organization. The source data for the study were collected by conducting a special survey of the organization within which the surveys were conducted, and interviewing of managers and employees of relevant structures of the research object, the study of local normative documents, regulations on departments, job descriptions, etc. The resulting set of artifacts of business architecture can be used further in the process of re-engineering business processes, developing indicators for the effectiveness functions, business processes, organization, and development of requirements for the application systems that automate the processing of data and development of subsequent domains of enterprise architecture

  10. Alginate production affects Pseudomonas aeruginosa biofilm development and architecture, but is not essential for biofilm formation

    DEFF Research Database (Denmark)

    Stapper, A.P.; Narasimhan, G.; Oman, D.E.;

    2004-01-01

    Extracellular polymers can facilitate the non-specific attachment of bacteria to surfaces and hold together developing biofilms. This study was undertaken to qualitatively and quantitatively compare the architecture of biofilms produced by Pseudomonas aeruginosa strain PAO1 and its alginate...... biofilm formation using confocal laser scanning microscopy. Biofilm Image Processing (BIP) and Community Statistics (COMSTAT) software programs were used to provide quantitative measurements of the two-dimensional biofilm images. All three strains formed distinguishable biofilm architectures, indicating...... that the production of alginate is not critical for biofilm formation. Observation over a period of 5 days indicated a three-stage development pattern consisting of initiation, establishment and maturation. Furthermore, this study showed that phenotypically distinguishable biofilms can be...

  11. Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid

    Directory of Open Access Journals (Sweden)

    Qu Jing

    2012-02-01

    Full Text Available Abstract Background Jatropha curcas is recognized as a new energy crop due to the presence of the high amount of oil in its seeds that can be converted into biodiesel. The quality and performance of the biodiesel depends on the chemical composition of the fatty acids present in the oil. The fatty acids profile of the oil has a direct impact on ignition quality, heat of combustion and oxidative stability. An ideal biodiesel composition should have more monounsaturated fatty acids and less polyunsaturated acids. Jatropha seed oil contains 30% to 50% polyunsaturated fatty acids (mainly linoleic acid which negatively impacts the oxidative stability and causes high rate of nitrogen oxides emission. Results The enzyme 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase (FAD2 is the key enzyme responsible for the production of linoleic acid in plants. We identified three putative delta 12 fatty acid desaturase genes in Jatropha (JcFAD2s through genome-wide analysis and downregulated the expression of one of these genes, JcFAD2-1, in a seed-specific manner by RNA interference technology. The resulting JcFAD2-1 RNA interference transgenic plants showed a dramatic increase of oleic acid (> 78% and a corresponding reduction in polyunsaturated fatty acids (Jatropha had around 37% oleic acid and 41% polyunsaturated fatty acids. This indicates that FAD2-1 is the major enzyme responsible for converting oleic acid to linoleic acid in Jatropha. Due to the changes in the fatty acids profile, the oil of the JcFAD2-1 RNA interference seed was estimated to yield a cetane number as high as 60.2, which is similar to the required cetane number for conventional premium diesel fuels (60 in Europe. The presence of high seed oleic acid did not have a negative impact on other Jatropha agronomic traits based on our preliminary data of the original plants under greenhouse conditions. Further, we developed a marker-free system to generate the transgenic Jatropha

  12. Desenvolvimento de frutos e sementes de tamarindo Development of tamarind fruits and seeds

    Directory of Open Access Journals (Sweden)

    Kátia Cristina de Oliveira Gurjão

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar as mudanças físicas e fisiológicas ocorridas durante o desenvolvimento de frutos e sementes de tamarindo. Foram selecionadas árvores de um pomar localizado na Escola Agrotécnica Federal de Sousa-PB, com 50% das inflorescências em antese, cujos ramos, contendo flores abertas, foram marcados, e as colheitas realizadas aos 20; 40; 70; 100; 130; 160; 190; 220; 250; 270 e 280 dias após a antese. A cada colheita, os frutos e as sementes foram submetidos às seguintes avaliações: comprimento, largura e espessura dos frutos e sementes (cm; massa da matéria seca dos frutos e sementes (g; teor de água das sementes (%; teste de germinação (%. As plantas de tamarindo levaram aproximadamente 280 dias após a antese para completar o ciclo desde a floração até a colheita, período representado pelo crescimento, maturação e o amadurecimento dos frutos. O crescimento dos frutos de tamarindo pode ser descrito por um modelo sigmoidal simples. A maturação fisiológica dos frutos de tamarindo ocorreu no período entre 270 e 280 dias após a antese, coincidindo com o desprendimento natural da planta-mãe e a maturação das sementes, aos 277 dias após a antese.The objective of this work was to evaluate the physical and physiologic changes happened during the development of tamarind fruits and seeds. Trees of a located orchard were selected at the Federal Agrothechnic School of Sousa-PB, with 50% of the inflorescences in anthesis, which branches, containing open flowers, were marked and the crops were accomplished to the 20, 40, 70, 100, 130, 160, 190, 220, 250, 270 and 280 days after anthesis. To each crop, fruits and seeds were submitted to the following evaluations: length, width and thickness of fruits and seeds (cm; fruits and seeds dry matter mass (g; seeds water content (% and germination test (%.Tamarind plants took approximately 280 days after anthesis to complete the cycle from blooming to crop

  13. An architecture and methodology for the design and development of Technical Information Systems

    OpenAIRE

    Capobianchi, R.; Mautref, M.; Keulen, van, Maurice; Balsters, H.

    1996-01-01

    In order to meet demands in the context of Technical Information Systems (TIS) pertaining to reliability, extensibility, maintainability, etc., we have developed an architectural framework with accompanying methodological guidelines for designing such systems. With the framework, we aim at complex multiapplication information systems using a repository to share data among applications. The framework proposes to keep a strict separation between Man-Machine-Interface and Model data, and provide...

  14. Model-Driven Game Development Addressing Architectural Diversity and Game Engine-Integration

    OpenAIRE

    Zhu, Meng

    2015-01-01

    Model-Driven Game Development (MDGD) is an emerging research field, which uses models to specify some or all of the game elements that traditionally had been manually coded. The PhD thesis presents research in the MDGD domain, which is intended to push MDGD further towards industrialization by reducing two gaps: 1) The integration of MDGD tools and game engines, and 2) The support for a diversity of game architectures. These gaps have been identified through a literature review...

  15. Exogenous glutamine increases lipid accumulation in developing seeds of castor bean (Ricinus communis L. cultured in vitro

    Directory of Open Access Journals (Sweden)

    Zhang Yang

    2015-01-01

    Full Text Available This report describes biomass production and compositional changes of developing castor seeds in response to change in the nitrogen resource (glutamine of the medium. During the early developmental period (24-36 days after pollination, oil was found to initially accumulate in the developing seeds. Carbohydrates and oil were inversely related after glutamine provision (35 mM, in the culture medium. [U-14C] sucrose labeling was used to investigate the effect of metabolic fluxes among different storage materials. Addition of glutamine led to a 7% increase of labeling in lipids and an inverse decrease of labeling in carbohydrates. It was postulated that changes in the glutamine concentration in the medium are likely to influence the partitioning of resources between the various storage products, especially carbohydrates and oil. These observations will contribute to a better understanding of assimilate partitioning in developing castor seeds and the development of molecular strategies to improve castor bean seed quality and plant breeding studies.

  16. Systemic Architecture

    DEFF Research Database (Denmark)

    Poletto, Marco; Pasquero, Claudia

    2012-01-01

    This is a manual investigating the subject of urban ecology and systemic development from the perspective of architectural design. It sets out to explore two main goals: to discuss the contemporary relevance of a systemic practice to architectural design, and to share a toolbox of informational...... design protocols developed to describe the city as a territory of self-organization. Collecting together nearly a decade of design experiments by the authors and their practice, ecoLogicStudio, the book discusses key disciplinary definitions such as ecologic urbanism, algorithmic architecture, bottom......-up or tactical design, behavioural space and the boundary of the natural and the artificial realms within the city and architecture. A new kind of "real-time world-city" is illustrated in the form of an operational design manual for the assemblage of proto-architectures, the incubation of proto...

  17. Development of the Lymphoma Enterprise Architecture Database: A caBIG(TM Silver Level Compliant System

    Directory of Open Access Journals (Sweden)

    Taoying Huang

    2009-01-01

    Full Text Available Lymphomas are the fifth most common cancer in United States with numerous histological subtypes. Integrating existing clinical information on lymphoma patients provides a platform for understanding biological variability in presentation and treatment response and aids development of novel therapies. We developed a cancer Biomedical Informatics Grid™ (caBIG™ Silver level compliant lymphoma database, called the Lymphoma Enterprise Architecture Data-system™ (LEAD™, which integrates the pathology, pharmacy, laboratory, cancer registry, clinical trials, and clinical data from institutional databases. We utilized the Cancer Common Ontological Representation Environment Software Development Kit (caCORE SDK provided by National Cancer Institute’s Center for Bioinformatics to establish the LEAD™ platform for data management. The caCORE SDK generated system utilizes an n-tier architecture with open Application Programming Interfaces, controlled vocabularies, and registered metadata to achieve semantic integration across multiple cancer databases. We demonstrated that the data elements and structures within LEAD™ could be used to manage clinical research data from phase 1 clinical trials, cohort studies, and registry data from the Surveillance Epidemiology and End Results database. This work provides a clear example of how semantic technologies from caBIG™ can be applied to support a wide range of clinical and research tasks, and integrate data from disparate systems into a single architecture. This illustrates the central importance of caBIG™ to the management of clinical and biological data.

  18. Communications System Architecture Development for Air Traffic Management and Aviation Weather Information Dissemination

    Science.gov (United States)

    Gallagher, Seana; Olson, Matt; Blythe, Doug; Heletz, Jacob; Hamilton, Griff; Kolb, Bill; Homans, Al; Zemrowski, Ken; Decker, Steve; Tegge, Cindy

    2000-01-01

    This document is the NASA AATT Task Order 24 Final Report. NASA Research Task Order 24 calls for the development of eleven distinct task reports. Each task was a necessary exercise in the development of comprehensive communications systems architecture (CSA) for air traffic management and aviation weather information dissemination for 2015, the definition of the interim architecture for 2007, and the transition plan to achieve the desired End State. The eleven tasks are summarized along with the associated Task Order reference. The output of each task was an individual task report. The task reports that make up the main body of this document include Task 5, Task 6, Task 7, Task 8, Task 10, and Task 11. The other tasks provide the supporting detail used in the development of the architecture. These reports are included in the appendices. The detailed user needs, functional communications requirements and engineering requirements associated with Tasks 1, 2, and 3 have been put into a relational database and are provided electronically.

  19. Development of the Lymphoma Enterprise Architecture Database: A caBIG(TM Silver Level Compliant System

    Directory of Open Access Journals (Sweden)

    Taoying Huang

    2009-04-01

    Full Text Available Lymphomas are the fifth most common cancer in United States with numerous histological subtypes. Integrating existing clinical information on lymphoma patients provides a platform for understanding biological variability in presentation and treatment response and aids development of novel therapies. We developed a cancer Biomedical Informatics Grid™ (caBIG™ Silver level compliant lymphoma database, called the Lymphoma Enterprise Architecture Data-system™ (LEAD™, which integrates the pathology, pharmacy, laboratory, cancer registry, clinical trials, and clinical data from institutional databases. We utilized the Cancer Common Ontological Representation Environment Software Development Kit (caCORE SDK provided by National Cancer Institute’s Center for Bioinformatics to establish the LEAD™ platform for data management. The caCORE SDK generated system utilizes an n-tier architecture with open Application Programming Interfaces, controlled vocabularies, and registered metadata to achieve semantic integration across multiple cancer databases. We demonstrated that the data elements and structures within LEAD™ could be used to manage clinical research data from phase 1 clinical trials, cohort studies, and registry data from the Surveillance Epidemiology and End Results database. This work provides a clear example of how semantic technologies from caBIG™ can be applied to support a wide range of clinical and research tasks, and integrate data from disparate systems into a single architecture. This illustrates the central importance of caBIG™ to the management of clinical and biological data.

  20. Development of the Lymphoma Enterprise Architecture Database: A caBIG(tm) Silver level compliant System

    Science.gov (United States)

    Huang, Taoying; Shenoy, Pareen J.; Sinha, Rajni; Graiser, Michael; Bumpers, Kevin W.; Flowers, Christopher R.

    2009-01-01

    Lymphomas are the fifth most common cancer in United States with numerous histological subtypes. Integrating existing clinical information on lymphoma patients provides a platform for understanding biological variability in presentation and treatment response and aids development of novel therapies. We developed a cancer Biomedical Informatics Grid™ (caBIG™) Silver level compliant lymphoma database, called the Lymphoma Enterprise Architecture Data-system™ (LEAD™), which integrates the pathology, pharmacy, laboratory, cancer registry, clinical trials, and clinical data from institutional databases. We utilized the Cancer Common Ontological Representation Environment Software Development Kit (caCORE SDK) provided by National Cancer Institute’s Center for Bioinformatics to establish the LEAD™ platform for data management. The caCORE SDK generated system utilizes an n-tier architecture with open Application Programming Interfaces, controlled vocabularies, and registered metadata to achieve semantic integration across multiple cancer databases. We demonstrated that the data elements and structures within LEAD™ could be used to manage clinical research data from phase 1 clinical trials, cohort studies, and registry data from the Surveillance Epidemiology and End Results database. This work provides a clear example of how semantic technologies from caBIG™ can be applied to support a wide range of clinical and research tasks, and integrate data from disparate systems into a single architecture. This illustrates the central importance of caBIG™ to the management of clinical and biological data. PMID:19492074

  1. Development of the Lymphoma Enterprise Architecture Database: a caBIG Silver level compliant system.

    Science.gov (United States)

    Huang, Taoying; Shenoy, Pareen J; Sinha, Rajni; Graiser, Michael; Bumpers, Kevin W; Flowers, Christopher R

    2009-01-01

    Lymphomas are the fifth most common cancer in United States with numerous histological subtypes. Integrating existing clinical information on lymphoma patients provides a platform for understanding biological variability in presentation and treatment response and aids development of novel therapies. We developed a cancer Biomedical Informatics Grid (caBIG) Silver level compliant lymphoma database, called the Lymphoma Enterprise Architecture Data-system (LEAD), which integrates the pathology, pharmacy, laboratory, cancer registry, clinical trials, and clinical data from institutional databases. We utilized the Cancer Common Ontological Representation Environment Software Development Kit (caCORE SDK) provided by National Cancer Institute's Center for Bioinformatics to establish the LEAD platform for data management. The caCORE SDK generated system utilizes an n-tier architecture with open Application Programming Interfaces, controlled vocabularies, and registered metadata to achieve semantic integration across multiple cancer databases. We demonstrated that the data elements and structures within LEAD could be used to manage clinical research data from phase 1 clinical trials, cohort studies, and registry data from the Surveillance Epidemiology and End Results database. This work provides a clear example of how semantic technologies from caBIG can be applied to support a wide range of clinical and research tasks, and integrate data from disparate systems into a single architecture. This illustrates the central importance of caBIG to the management of clinical and biological data. PMID:19492074

  2. The role of photosynthesis and amino acid metabolism in the energy status during seed development

    OpenAIRE

    Galili, Gad; Avin-Wittenberg, Tamar; Angelovici, Ruthie; Fernie, Alisdair R

    2014-01-01

    Seeds are the major organs responsible for the evolutionary upkeep of angiosperm plants. Seeds accumulate significant amounts of storage compounds used as nutrients and energy reserves during the initial stages of seed germination. The accumulation of storage compounds requires significant amounts of energy, the generation of which can be limited due to reduced penetration of oxygen and light particularly into the inner parts of seeds. In this review, we discuss the adjustment of seed metabol...

  3. An expression analysis of 57 transcription factors derived from ESTs of developing seeds in Maize (Zea mays).

    Science.gov (United States)

    Wang, Guifeng; Wang, Hui; Zhu, Jia; Zhang, Jing; Zhang, Xiaowei; Wang, Fei; Tang, Yuanping; Mei, Bing; Xu, Zhengkai; Song, Rentao

    2010-06-01

    Maize seeds are an important source of food, animal feed, and industrial raw materials. To understand global gene expression and regulation during maize seed development, a normalized cDNA library, covering most of the developmental stages of maize seeds, was constructed. Sequencing analysis of 10,848 randomly selected clones identified 6,630 unique ESTs. Among them, 57 putative transcription factors (TFs) were identified. The TFs belong to seven different super-families, specifically 17 Zinc-finger, 13 bZIP, 8 bHLH, 6 MADS, 7 MYB, 3 Homedomain, and 3 AP2/EREBP. The spatial and temporal expression of the TFs was analyzed by semi-quantitative RT-PCR with representative tissue types and seeds at different developmental stages, revealing their diverse expression patterns and expression levels. One-third (19) of the maize TFs was found their putative orthologs in Arabidopsis. Similar expression patterns were observed in both maize and Arabidopsis for the majority of orthologous pairs (15 out of 19), suggesting their conserved functions during seed development. In conclusion, the systematic analysis of maize seed TFs has provided valuable insight into transcriptional regulation during maize seed development. PMID:20336461

  4. Water relations in seed biology

    OpenAIRE

    Villela F. A.

    1998-01-01

    The water relations play a fundamental role in seed biology. Thus, the purpose of the present paper was to analyze the performance of water status in seed development and germination. The researches have suggested that the water potential of the seed or seed structures provides a better indicator of the seed water status than water content. The seed water status plays a regulatory role in seed development and germination.

  5. Exogenous glutamine increases lipid accumulation in developing seeds of castor bean (Ricinus communis L.) cultured in vitro

    OpenAIRE

    Zhang Yang; Mulpuri Sujatha; Liu Aizhong

    2015-01-01

    This report describes biomass production and compositional changes of developing castor seeds in response to change in the nitrogen resource (glutamine) of the medium. During the early developmental period (24-36 days after pollination), oil was found to initially accumulate in the developing seeds. Carbohydrates and oil were inversely related after glutamine provision (35 mM, in the culture medium). [U-14C] sucrose labeling was used to investigate the effe...

  6. Judicious use of custom development in an open source component architecture

    Science.gov (United States)

    Bristol, S.; Latysh, N.; Long, D.; Tekell, S.; Allen, J.

    2014-12-01

    Modern software engineering is not as much programming from scratch as innovative assembly of existing components. Seamlessly integrating disparate components into scalable, performant architecture requires sound engineering craftsmanship and can often result in increased cost efficiency and accelerated capabilities if software teams focus their creativity on the edges of the problem space. ScienceBase is part of the U.S. Geological Survey scientific cyberinfrastructure, providing data and information management, distribution services, and analysis capabilities in a way that strives to follow this pattern. ScienceBase leverages open source NoSQL and relational databases, search indexing technology, spatial service engines, numerous libraries, and one proprietary but necessary software component in its architecture. The primary engineering focus is cohesive component interaction, including construction of a seamless Application Programming Interface (API) across all elements. The API allows researchers and software developers alike to leverage the infrastructure in unique, creative ways. Scaling the ScienceBase architecture and core API with increasing data volume (more databases) and complexity (integrated science problems) is a primary challenge addressed by judicious use of custom development in the component architecture. Other data management and informatics activities in the earth sciences have independently resolved to a similar design of reusing and building upon established technology and are working through similar issues for managing and developing information (e.g., U.S. Geoscience Information Network; NASA's Earth Observing System Clearing House; GSToRE at the University of New Mexico). Recent discussions facilitated through the Earth Science Information Partners are exploring potential avenues to exploit the implicit relationships between similar projects for explicit gains in our ability to more rapidly advance global scientific cyberinfrastructure.

  7. Stenospermy and seed development in the "Brazilian seedless" variety of sugar apple (Annona squamosa).

    Science.gov (United States)

    Dos Santos, Rayane C; Ribeiro, Leonardo M; Mercadante-Simões, Maria Olívia; Costa, Márcia R; Nietsche, Silvia; Pereira, Marlon C T

    2014-12-01

    Stenospermy was identified in naturally occurring sugar-apple (Annona squamosa) mutants with great potential for use in genetic improvement programs. However, to date, there have been no detailed studies of the development of aspermic fruit in this species. The aim of the present study was to characterize the anatomy of developing fruit in the 'Brazilian Seedless' mutant. Flower buds in pre-anthesis and developing fruits were subjected to common plant anatomy techniques. The abnormal ovules are unitegmic and orthotropic and have a long funiculus. There is evidence of fertilization, including the presence of embryos in early development and the proliferation of starch grains in the embryo sac. However, the embryos and embryo sac degenerate, although this does not affect pericarp development. Ovule abortion does not occur. The perisperm, which is formed from the peripheral layers of the nucellus, fills the cavity left by the embryo sac. The mature fruit contains numerous small sterile seeds with abundant perisperm and unlignified integument that is restricted to the micropylar region. The majority of perisperm cells are living and appear to be metabolically active in the periphery. Therefore, stenospermy leads to the formation of sterile seeds in A. squamosa, and the perisperm possibly play an important role in fruit development. PMID:25590744

  8. Dynamic Metabolic Profiles and Tissue-Specific Source Effects on the Metabolome of Developing Seeds of Brassica napus.

    Directory of Open Access Journals (Sweden)

    Helin Tan

    Full Text Available Canola (Brassica napus is one of several important oil-producing crops, and the physiological processes, enzymes, and genes involved in oil synthesis in canola seeds have been well characterized. However, relatively little is known about the dynamic metabolic changes that occur during oil accumulation in seeds, as well as the mechanistic origins of metabolic changes. To explore the metabolic changes that occur during oil accumulation, we isolated metabolites from both seed and silique wall and identified and characterized them by using gas chromatography coupled with mass spectrometry (GC-MS. The results showed that a total of 443 metabolites were identified from four developmental stages. Dozens of these metabolites were differentially expressed during seed ripening, including 20 known to be involved in seed development. To investigate the contribution of tissue-specific carbon sources to the biosynthesis of these metabolites, we examined the metabolic changes of silique walls and seeds under three treatments: leaf-detachment (Ld, phloem-peeling (Pe, and selective silique darkening (Sd. Our study demonstrated that the oil content was independent of leaf photosynthesis and phloem transport during oil accumulation, but required the metabolic influx from the silique wall. Notably, Sd treatment resulted in seed senescence, which eventually led to a severe reduction of the oil content. Sd treatment also caused a significant accumulation of fatty acids (FA, organic acids and amino acids. Furthermore, an unexpected accumulation of sugar derivatives and organic acid was observed in the Pe- and Sd-treated seeds. Consistent with this, the expression of a subset of genes involved in FA metabolism, sugar and oil storage was significantly altered in Pe and Sd treated seeds. Taken together, our studies suggest the metabolite profiles of canola seeds dynamically varied during the course of oil accumulation, which may provide a new insight into the mechanisms

  9. DENDRITIC ARCHITECTURE: A NEW TOOL FOR DEVELOPMENT OF NOVEL DRUG DELIVERY SYSTEMS

    Directory of Open Access Journals (Sweden)

    Ram Kumar Choudhary

    2012-02-01

    Full Text Available Dendrimers are composed of a multifunctional core molecule attached to polymer branches. Drugs can be inserted between the branches and held until ‘interactions’ release it. Dendrimers are a new class of three-dimensional, man-made molecules produced by an unusual synthetic route which incorporates repetitive branching sequences to create a unique novel architecture. Exceptional features of the dendritic architecture include a high degree of structural symmetry, a density gradient displaying an intra-molecular minimum value and a well defined number of terminal groups which may be chemically different from the interior. The combination of these features creates an environment within the dendrimer molecule facilitates an avenue to developing reliable and economical fabrication and manufacturing of functional nanoscale materials that would have unique properties (electronic, optical, opto-electronic, magnetic, chemical, or biological that could be the basis of new nanoscale technology and devices.

  10. Development of a structured observational method for the systematic assessment of school food-choice architecture.

    Science.gov (United States)

    Ozturk, Orgul D; McInnes, Melayne M; Blake, Christine E; Frongillo, Edward A; Jones, Sonya J

    2016-01-01

    The objective of this study is to develop a structured observational method for the systematic assessment of the food-choice architecture that can be used to identify key points for behavioral economic intervention intended to improve the health quality of children's diets. We use an ethnographic approach with observations at twelve elementary schools to construct our survey instrument. Elements of the structured observational method include decision environment, salience, accessibility/convenience, defaults/verbal prompts, number of choices, serving ware/method/packaging, and social/physical eating environment. Our survey reveals important "nudgeable" components of the elementary school food-choice architecture, including precommitment and default options on the lunch line. PMID:26654767

  11. Development of automated measurement system for radioactive intensities of sealed small radiation sources (iodine-125 seed source) for brachytherapy

    International Nuclear Information System (INIS)

    We have developed full automated measurement system for radioactive intensities of sealed small radiation sources (iodine-125 seed source) for brachytherapy in this work. Today, quality assurance (QA) of I-125 seed radioactive sources for brachytherapy following AAPM (American Association of Physicists in Medicine) Society's guideline is one of important subjects for hospitals that operate on patients for prostate cancer and medical companies that manufacture and sell these radioactive sources. In order to survey defective seed products into all the number of I-125 seed sources (there are usually fifteen seeds, one seed of dimensions is 0.8 mm φ × 4.5 mm length) within a cartridge, we have applied the method of single slit collimator with moving a radiation detector to measure each radioactive intensity of these I-125 seeds. As a result, it was found that our developed system in the present work has good performance of surveying the defective products manufactured with radioactive intensities out of about ±15% error (p < 0.05). (author)

  12. A development architecture for serious games using BCI (brain computer interface) sensors.

    Science.gov (United States)

    Sung, Yunsick; Cho, Kyungeun; Um, Kyhyun

    2012-01-01

    Games that use brainwaves via brain-computer interface (BCI) devices, to improve brain functions are known as BCI serious games. Due to the difficulty of developing BCI serious games, various BCI engines and authoring tools are required, and these reduce the development time and cost. However, it is desirable to reduce the amount of technical knowledge of brain functions and BCI devices needed by game developers. Moreover, a systematic BCI serious game development process is required. In this paper, we present a methodology for the development of BCI serious games. We describe an architecture, authoring tools, and development process of the proposed methodology, and apply it to a game development approach for patients with mild cognitive impairment as an example. This application demonstrates that BCI serious games can be developed on the basis of expert-verified theories. PMID:23202227

  13. A Development Architecture for Serious Games Using BCI (Brain Computer Interface Sensors

    Directory of Open Access Journals (Sweden)

    Kyhyun Um

    2012-11-01

    Full Text Available Games that use brainwaves via brain–computer interface (BCI devices, to improve brain functions are known as BCI serious games. Due to the difficulty of developing BCI serious games, various BCI engines and authoring tools are required, and these reduce the development time and cost. However, it is desirable to reduce the amount of technical knowledge of brain functions and BCI devices needed by game developers. Moreover, a systematic BCI serious game development process is required. In this paper, we present a methodology for the development of BCI serious games. We describe an architecture, authoring tools, and development process of the proposed methodology, and apply it to a game development approach for patients with mild cognitive impairment as an example. This application demonstrates that BCI serious games can be developed on the basis of expert-verified theories.

  14. Development of procedure using plasma welding process to produce 125I seeds

    International Nuclear Information System (INIS)

    The prostate cancer, which is the second cause of death by cancer in men, overcome only by lung cancer, is a problem of public health in Brazil. Brachytherapy is among the possible available treatments for prostate cancer, in which small seeds containing 125I radioisotope are implanted in the prostate. The seed consists of a titanium sealed capsule with 0.8 mm external diameter and 4.5 mm length, containing a central silver wire with adsorbed 125I. The plasma arc welding is one of the viable techniques for the sealing process. The equipment used in this technique is less costly than in other processes. The main objective of this work was the development and the validation of the welding procedure using plasma welding process and the elaboration of a sealing routine according to Good Manufacturing Practices. The development of this work has presented the following phases: cut and cleaning of the titanium material, determination of the welding parameters, development of a device for holding the titanium tube during the welding process, validation of sealed sources according to ISO 2919 Sealed Radioactive Sources - General Requirements and Classification, leakage test according to ISO 9978 Sealed Radioactive Sources - Leakage Test Methods and metallographic assays. The developed procedure, to seal 125I seeds using plasma welding process, has shown to be efficient, satisfying all the established requirements of ISO 2919. The results obtained in this work have given the possibility to establish a routine production process according to the orientations presented in resolution RDC number 59 - Good Manufacturing Practices do Medical Products of the ANVISA - Brazilian Nacional Agency of Sanitary Surveillance. (author)

  15. Software architecture 2

    CERN Document Server

    Oussalah, Mourad Chabanne

    2014-01-01

    Over the past 20 years, software architectures have significantly contributed to the development of complex and distributed systems. Nowadays, it is recognized that one of the critical problems in the design and development of any complex software system is its architecture, i.e. the organization of its architectural elements. Software Architecture presents the software architecture paradigms based on objects, components, services and models, as well as the various architectural techniques and methods, the analysis of architectural qualities, models of representation of architectural templa

  16. Software architecture 1

    CERN Document Server

    Oussalah , Mourad Chabane

    2014-01-01

    Over the past 20 years, software architectures have significantly contributed to the development of complex and distributed systems. Nowadays, it is recognized that one of the critical problems in the design and development of any complex software system is its architecture, i.e. the organization of its architectural elements. Software Architecture presents the software architecture paradigms based on objects, components, services and models, as well as the various architectural techniques and methods, the analysis of architectural qualities, models of representation of architectural template

  17. Logic of historical development of the formation process of architectural and construction solutions

    Directory of Open Access Journals (Sweden)

    Baranov Valeriy Aleksandrovich

    2014-05-01

    Full Text Available In research field of development processes of architectural and construction decisions (ACD there is already very considerable scientific, normative and technical material. And still in the end of the research boom in this area (the 90th many authors stated their weak return in practical design. High methodological potential gives understanding of the act of ACD formation as a special kind of activity, and its structure - as historical phenomenon that allows to put two main methodological principles in the basis of the research: the principle of activity and the principle of level organization of historically formed objects. As a result it was succeeded to reveal 6 main stages of historical development of ACD formation process and 6 levels of its modern organization. Origin stage - is the transition from animal to human construction and emergence of the first stage of ACD formation - a measurement. The reproductive stage is characterized by centuries-old reproduction of steady volume forms of constructions. At a composite stage professional presentation detaches external form of a construction from architectural and construction actions and techniques. There occurs transformation into a subject of architectural and construction activity and creation of a method of composition on this basis. At a constructive stage engineers are involved in construction process, there is a change of style of thinking, emergence of construction designs and emergence of a new function of ACD means of configuration, and, at last, final allocation and separation of architectural and construction design from construction. Technological stage is a product of industrialization of construction on which systematization is carried out not only on the level of subject, but also in respect to the operational contents that leads to emergence of ACD program formation. At the sixth, methodological stage of development of ASP, a leading way change of the ACD formation becomes a

  18. A study of ovule-to-seed development in Ceratiosicyos (Achariaceae and the systematic position of the genus

    Directory of Open Access Journals (Sweden)

    E. M. A. Steyn

    2002-12-01

    Full Text Available A light microscope study of developing ovules and seeds of Ceratiosicyos laevis (Thunb. A.Meeuse was undertaken to aug­ment an investigation of ovule and seed structure in Achariaceae. a tri-generic family comprising three species of herbaceous perennials endemic to southern Africa. Tests for myrmecochory suggest that seed of Ceratiosicyos Nees is not dispersed by ants like those of Acharia Thunb. and Guthriea Bolus. Structural differences include the absence of a raphal ridge and imbibition lidand the presence of long funicles and medium-sized embryos in Ceratiosicyos.

  19. “Pushing the Envelope” a modeling-based approach to the development of organic, responsive architectural form

    OpenAIRE

    David Yearley

    2012-01-01

    This paper tests design procedures for the development of complex, organic architectural forms. It illustrates a postgraduate student design process, implementing a development sequence based on the intelligent manipulation of architectural envelopes using a variety of existing modeling tools and emerging digital techniques. These stages of development respond to imposed spatial and environmental constraints. The tests began with full-scale modeling of small segments. The major constraints at...

  20. A METHOD FOR THE DEVELOPMENT OF PLATFORM MODELS IN THE MODEL DRIVEN ARCHITECTURE CONTEXT

    OpenAIRE

    Paulo Cezar Stadzisz; Luciane Telinski Wiedermann Agner; Inali Wisniewski Soares; Jean Marcelo Simao

    2012-01-01

    The application of the Model Driven Architecture (MDA) approach to the design of embedded software based on Real-Time Operating Systems (RTOS) is encouraged by the fact that such software has a wide variety of platforms. In this way, the creation of methods for the development of platform models that meet such diversity of platforms is necessary. This study proposes a method called PM-MDA for the development of platform models that design embedded software based on RTOS in the context of MDA....

  1. SEEDSTICK is a master regulator of development and metabolism in the Arabidopsis seed coat.

    Directory of Open Access Journals (Sweden)

    Chiara Mizzotti

    2014-12-01

    Full Text Available The role of secondary metabolites in the determination of cell identity has been an area of particular interest over recent years, and studies strongly indicate a connection between cell fate and the regulation of enzymes involved in secondary metabolism. In Arabidopsis thaliana, the maternally derived seed coat plays pivotal roles in both the protection of the developing embryo and the first steps of germination. In this regard, a characteristic feature of seed coat development is the accumulation of proanthocyanidins (PAs - a class of phenylpropanoid metabolites in the innermost layer of the seed coat. Our genome-wide transcriptomic analysis suggests that the ovule identity factor SEEDSTICK (STK is involved in the regulation of several metabolic processes, providing a strong basis for a connection between cell fate determination, development and metabolism. Using phenotypic, genetic, biochemical and transcriptomic approaches, we have focused specifically on the role of STK in PA biosynthesis. Our results indicate that STK exerts its effect by direct regulation of the gene encoding BANYULS/ANTHOCYANIDIN REDUCTASE (BAN/ANR, which converts anthocyanidins into their corresponding 2,3-cis-flavan-3-ols. Our study also demonstrates that the levels of H3K9ac chromatin modification directly correlate with the active state of BAN in an STK-dependent way. This is consistent with the idea that MADS-domain proteins control the expression of their target genes through the modification of chromatin states. STK might thus recruit or regulate histone modifying factors to control their activity. In addition, we show that STK is able to regulate other BAN regulators. Our study demonstrates for the first time how a floral homeotic gene controls tissue identity through the regulation of a wide range of processes including the accumulation of secondary metabolites.

  2. Proteome analysis of plastids from developing seeds of Jatropha curcas L.

    Science.gov (United States)

    Pinheiro, Camila B; Shah, Mohibullah; Soares, Emanoella L; Nogueira, Fábio C S; Carvalho, Paulo C; Junqueira, Magno; Araújo, Gabriel D T; Soares, Arlete A; Domont, Gilberto B; Campos, Francisco A P

    2013-11-01

    In this study, we performed a proteomic analysis of plastids isolated from the endosperm of developing Jatropha curcas seeds that were in the initial stage of deposition of protein and lipid reserves. Proteins extracted from the plastids were digested with trypsin, and the peptides were applied to an EASY-nano LC system coupled inline to an ESI-LTQ-Orbitrap Velos mass spectrometer, and this led to the identification of 1103 proteins representing 804 protein groups, of which 923 proteins were considered as true identifications, and this considerably expands the repertoire of J. curcas proteins identified so far. Of the identified proteins, only five are encoded in the plastid genome, and none of them are involved in photosynthesis, evidentiating the nonphotosynthetic nature of the isolated plastids. Homologues for 824 out of 923 identified proteins were present in PPDB, SUBA, or PlProt databases while homologues for 13 proteins were not found in any of the three plastid proteins databases but were marked as plastidial by at least one of the three prediction programs used. Functional classification showed that proteins belonging to amino acids metabolism comprise the main functional class, followed by carbohydrate, energy, and lipid metabolisms. The small and large subunits of Rubisco were identified, and their presence in the plastids is considered to be an adaptive feature counterbalancing for the loss of one-third of the carbon as CO2 as a result of the conversion of carbohydrate to oil through glycolysis. While several enzymes involved in the biosynthesis of several precursors of diterpenoids were identified, we were unable to identify any terpene synthase/cyclase, which suggests that the plastids isolated from the endosperm of developing seeds do not synthesize phorbol esters. In conclusion, our study provides insights into the major biosynthetic pathways and certain unique features of the plastids from the endosperm of developing seeds at the whole proteome

  3. Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing

    Directory of Open Access Journals (Sweden)

    Chen Shou-Yi

    2011-01-01

    Full Text Available Abstract Background MicroRNAs (miRNAs regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in higher plants. miRNAs and related target genes have been widely studied in model plants such as Arabidopsis and rice; however, the number of identified miRNAs in soybean (Glycine max is limited, and global identification of the related miRNA targets has not been reported in previous research. Results In our study, a small RNA library and a degradome library were constructed from developing soybean seeds for deep sequencing. We identified 26 new miRNAs in soybean by bioinformatic analysis and further confirmed their expression by stem-loop RT-PCR. The miRNA star sequences of 38 known miRNAs and 8 new miRNAs were also discovered, providing additional evidence for the existence of miRNAs. Through degradome sequencing, 145 and 25 genes were identified as targets of annotated miRNAs and new miRNAs, respectively. GO analysis indicated that many of the identified miRNA targets may function in soybean seed development. Additionally, a soybean homolog of Arabidopsis SUPPRESSOR OF GENE SLIENCING 3 (AtSGS3 was detected as a target of the newly identified miRNA Soy_25, suggesting the presence of feedback control of miRNA biogenesis. Conclusions We have identified large numbers of miRNAs and their related target genes through deep sequencing of a small RNA library and a degradome library. Our study provides more information about the regulatory network of miRNAs in soybean and advances our understanding of miRNA functions during seed development.

  4. Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds.

    Science.gov (United States)

    Andrade, T; Von Pinho, E V R; Von Pinho, R G; Oliveira, G E; Andrade, V; Fernandes, J S

    2013-01-01

    We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test. The seeds were stored in a freezer for later analysis of expression of heat-resistant proteins by means of real-time PCR, electrophoresis, and spectrophotometry. We observed that heat-resistant proteins are expressed in a differential manner in seeds from different lines and at different stages of development. The expression of heat-resistant proteins was earlier in lines tolerant to high drying temperatures. Greater germination and vigor values was found for seeds collected at the last stage of development. PMID:24085427

  5. Developing a real-time emulation of multiresolutional control architectures for complex, discrete-event systems

    Energy Technology Data Exchange (ETDEWEB)

    Davis, W.J.; Macro, J.G.; Brook, A.L. [Univ. of Illinois, Urbana, IL (United States)] [and others

    1996-12-31

    This paper first discusses an object-oriented, control architecture and then applies the architecture to produce a real-time software emulator for the Rapid Acquisition of Manufactured Parts (RAMP) flexible manufacturing system (FMS). In specifying the control architecture, the coordinated object is first defined as the primary modeling element. These coordinated objects are then integrated into a Recursive, Object-Oriented Coordination Hierarchy. A new simulation methodology, the Hierarchical Object-Oriented Programmable Logic Simulator, is then employed to model the interactions among the coordinated objects. The final step in implementing the emulator is to distribute the models of the coordinated objects over a network of computers and to synchronize their operation to a real-time clock. The paper then introduces the Hierarchical Subsystem Controller as an intelligent controller for the coordinated object. The proposed approach to intelligent control is then compared to the concept of multiresolutional semiosis that has been developed by Dr. Alex Meystel. Finally, the plans for implementing an intelligent controller for the RAMP FMS are discussed.

  6. Development of computational models for the simulation of isodose curves on dosimetry films generated by iodine-125 brachytherapy seeds

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Adriano M.; Meira-Belo, Luiz C.; Reis, Sergio C.; Grynberg, Suely E., E-mail: amsantos@cdtn.b [Center for Development of Nuclear Technology (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2011-07-01

    The interstitial brachytherapy is one modality of radiotherapy in which radioactive sources are placed directly in the region to be treated or close to it. The seeds that are used in the treatment of prostate cancer are generally cylindrical radioactive sources, consisting of a ceramic or metal matrix, which acts as the carrier of the radionuclide and as the X-ray marker, encapsulated in a sealed titanium tube. This study aimed to develop a computational model to reproduce the film-seed geometry, in order to obtain the spatial regions of the isodose curves produced by the seed when it is put over the film surface. The seed modeled in this work was the OncoSeed 6711, a sealed source of iodine-125, which its isodose curves were obtained experimentally in previous work with the use of dosimetric films. For the films modeling, compositions and densities of the two types of dosimetric films were used: Agfa Personal Monitoring photographic film 2/10, manufactured by Agfa-Geavaert; and the model EBT radiochromic film, by International Specialty Products. The film-seed models were coupled to the Monte Carlo code MCNP5. The results obtained by simulations showed to be in good agreement with experimental results performed in a previous work. This indicates that the computational model can be used in future studies for other seeds models. (author)

  7. Development of Advanced Seed Laser Modules for Lidar and Spectroscopy Applications

    Science.gov (United States)

    Prasad, Narasimha S.; Rosiewicz, Alex; Coleman, Steven M.

    2013-01-01

    We report on recent progress made in the development of highly compact, single mode, distributed feedback laser (DFB) seed laser modules for lidar and spectroscopy applications from space based platforms. One of the intended application of this technology is in the NASA's Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) mission. The DFB laser modules operating at 1571 nm and 1262 nm have advanced current and temperature drivers built into them. A combination of temperature and current tuning allows coarse and fine adjustment of the diode wavelengths.

  8. Arabidopsis CPR5 Independently Regulates Seed Germination and Postgermination Arrest of Development through LOX Pathway and ABA Signaling

    OpenAIRE

    Guilan Gao; Shengchun Zhang; Chengfeng Wang; Xiang Yang; Yaqin Wang; Xiaojun Su; Jinju Du; Chengwei Yang

    2011-01-01

    The phytohormone abscisic acid (ABA) and the lipoxygenases (LOXs) pathway play important roles in seed germination and seedling growth and development. Here, we reported on the functional characterization of Arabidopsis CPR5 in the ABA signaling and LOX pathways. The cpr5 mutant was hypersensitive to ABA in the seed germination, cotyledon greening and root growth, whereas transgenic plants overexpressing CPR5 were insensitive. Genetic analysis demonstrated that CPR5 gene may be located downst...

  9. Soybean roots retain the seed urease isozyme synthesized during embryo development

    International Nuclear Information System (INIS)

    Roots of young soybean (Glycine max [L.] Merr.) plants (up to 25 days old) contain two distinct urease isozymes, which are separable by hydroxyapatite chromatography. These two urease species (URE1 and URE2) differ in: (a) electrophoretic mobility in native gels, (b) pH dependence, and (c) recognition by a monoclonal antibody specific for the seed (embryo-specific) urease. By these parameters root URE1 urease is similar to the abundant embryo-specific urease isozyme, while root URE2 resembles the ubiquitous urease which has previously been found in all soybean tissues examined (leaf, embryo, seed coat, and cultured cells). The embryo-specific and ubiquitous urease isozymes are products of the Eu1 and Eu4 structural genes, respectively. Roots of the eu1-sun/eu1-sun genotype, which lacks the embryo-specific urease (i.e. seed urease-null), contain no URE1 urease activity. Roots of eu4/eu4, which lacks ubiquitous urease, lack the URE2 (leaflike) urease activity. From these genetic and biochemical criteria, then, we conclude that URE1 and URE2 are the embryo-specific and ubiquitous ureases, respectively. Adventitious roots generated from cuttings of any urease genotype lack URE1 activity. In seedling roots the seedlike (URE1) activity declines during development. Roots of 3-week-old plants contain 5% of the total URE1 activity of the radicle of 4-day-old seedlings, which, in turn, has approximately the same urease activity level as the dormant embryonic axis. The embryo-specific urease incorporates label from [35S]methionine during embryo development but not during germination, indicating that there is no de novo synthesis of the embryo-specific (URE1) urease in the germinating root

  10. Oxygen-depleted zones inside reproductive structures of Brassicaceae: implications for oxygen control of seed development

    Science.gov (United States)

    Porterfield, D. M.; Kuang, A.; Smith, P. J.; Crispi, M. L.; Musgrave, M. E.

    1999-01-01

    Growth of Arabidopsis thaliana (L.) Heynh. in decreasing oxygen partial pressures revealed a linear decrease in seed production below 15 kPa, with a complete absence of seed production at 2.5 kPa oxygen. This control of plant reproduction by oxygen had previously been attributed to an oxygen effect on the partitioning between vegetative and reproductive growth. However, plants grown in a series of decreasing oxygen concentrations produced progressively smaller embryos that had stopped developing at progressively younger stages, suggesting instead that their growth is limited by oxygen. Internal oxygen concentrations of buds, pistils, and developing siliques of Brassica rapa L. and siliques of Arabidopsis were measured using a small-diameter glass electrode that was moved into the structures using a micromanipulator. Oxygen partial pressures were found to be lowest in the developing perianth (11.1 kPa) and pistils (15.2 kPa) of the unopened buds. Pollination reduced oxygen concentration inside the pistils by 3 kPa after just 24 h. Inside Brassica silique locules, partial pressures of oxygen averaged 12.2 kPa in darkness, and increased linearly with increasing light levels to 16.2 kPa. Measurements inside Arabidopsis siliques averaged 6.1 kPa in the dark and rose to 12.2 kPa with light. Hypoxia in these microenvironments is postulated to be the point of control of plant reproduction by oxygen.

  11. Arabidopsis inositol 1,3,4-trisphosphate 5/6 kinase 2 is required for seed coat development

    Institute of Scientific and Technical Information of China (English)

    Yong Tang; Shutang Tan; Hongwei Xue

    2013-01-01

    Inositol 1,3,4-trisphosphate 5/6 kinase (ITPK) phosphorylates inositol 1,3,4-trisphosphate to form inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4,6-tetrakisphosphate which can be finally transferred to inositoi hexaphosphate (IP6) and play important roles during plant growth and development.There are 4 putative ITPK members in Arabidopsis.Expression pattern analysis showed that ITPK2 is constitutively expressed in various tissues.A TDNA knockout mutant of ITPK2 was identified and scanning electron microscopy (SEM) analysis showed that the epidermis structure of seed coat was irregularly formed in seeds of itpk2-1 mutant,resulting in the increased permeability of seed coat to tetrazolium salts.Further analysis by gas chromatography coupled with mass spectrometry of lipid polyester monomers in cell wall confirmed a dramatic decrease in composition of suberin and cutin,which relate to the permeability of seed coat and the formation of which is accompanied with seed coat development.These results indicate that ITPK2 plays an essential role in seed coat development and lipid polyester barrier formation.

  12. Biosynthesis of raffinose family oligosaccharides and galactosyl pinitols in developing and maturing seeds of winter vetch (Vicia vlllosa Roth.

    Directory of Open Access Journals (Sweden)

    Lesław B. Lahuta

    2011-04-01

    Full Text Available Changes in the accumulation of two types of α-D-galactosides: raffinose family oligosaccharides and galactosyl pinitols were compared with changes in the activities of galactosyltransferases during winter vetch (Vicia villosa Roth. seed development and maturation. Occurrence of galactinol and raffinose in young seeds and changes in activities of galactinol synthase and raffinose synthase during seed development indicated that formation of raffinose oligosaccharides (RFOs preceded synthesis of galactopinitols. Although transfer of galactose residues into raffinose oligosaccharides increased as seeds were maturing, at late stages of seed maturation the accumulation of galactopinitols was preferred to that of RFOs. In the present study, activities of enzymes transferring galactose moieties from galactinol to D-pinitol forming galactopinitol A, and further transfer of galactose moieties from galactinol to mono- and di-galactopinitol A were detected throughout seed development and maturation. This is a new observation, indicating biological potential of winter vetch seeds to synthesize mono-, di- and tri-galactosides of D-pinitol in a pathway similar to RFOs. The pattern of changes in activities of stachyose synthase and enzymes synthesizing galactopinitols (named galactopinitol A synthase and ciceritol synthase suggests that formation of stachyose, mono- and di-galactopinitol A (ciceritol is catalyzed by one enzyme. High correlation between activities of verbascose synthase and enzyme catalyzing synthesis of tri-galactopinitol A from galactinol and ciceritol (named tri-galactopinitol A synthase also suggests that biosynthesis of both types of tri-galactosides was catalyzed by one enzyme, but distinct from stachyose synthase. Changes in concentrations of galactosyl acceptors (sucrose and D-pinitol can be a factor which regulates splitting of galactose moieties between both types of galactosides in winter vetch seeds.

  13. Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds

    Directory of Open Access Journals (Sweden)

    Seabra Ana R

    2010-08-01

    Full Text Available Abstract Background Nitrogen is a crucial nutrient that is both essential and rate limiting for plant growth and seed production. Glutamine synthetase (GS, occupies a central position in nitrogen assimilation and recycling, justifying the extensive number of studies that have been dedicated to this enzyme from several plant sources. All plants species studied to date have been reported as containing a single, nuclear gene encoding a plastid located GS isoenzyme per haploid genome. This study reports the existence of a second nuclear gene encoding a plastid located GS in Medicago truncatula. Results This study characterizes a new, second gene encoding a plastid located glutamine synthetase (GS2 in M. truncatula. The gene encodes a functional GS isoenzyme with unique kinetic properties, which is exclusively expressed in developing seeds. Based on molecular data and the assumption of a molecular clock, it is estimated that the gene arose from a duplication event that occurred about 10 My ago, after legume speciation and that duplicated sequences are also present in closely related species of the Vicioide subclade. Expression analysis by RT-PCR and western blot indicate that the gene is exclusively expressed in developing seeds and its expression is related to seed filling, suggesting a specific function of the enzyme associated to legume seed metabolism. Interestingly, the gene was found to be subjected to alternative splicing over the first intron, leading to the formation of two transcripts with similar open reading frames but varying 5' UTR lengths, due to retention of the first intron. To our knowledge, this is the first report of alternative splicing on a plant GS gene. Conclusions This study shows that Medicago truncatula contains an additional GS gene encoding a plastid located isoenzyme, which is functional and exclusively expressed during seed development. Legumes produce protein-rich seeds requiring high amounts of nitrogen, we postulate

  14. A Reference Architecture for Providing Tools as a Service to Support Global Software Development

    DEFF Research Database (Denmark)

    Chauhan, Aufeef

    2014-01-01

    -computing paradigm for addressing above-mentioned issues by providing a framework to select appropriate tools as well as associated services and reference architecture of the cloud-enabled middleware platform that allows on demand provisioning of software engineering Tools as a Service (TaaS) with focus on......Global Software Development (GSD) teams encounter challenges that are associated with distribution of software development activities across multiple geographic regions. The limited support for performing collaborative development and engineering activities and lack of sufficient support for......-based solutions. The restricted ability of the organizations to have desired alignment of tools with software engineering and development processes results in administrative and managerial overhead that incur increased development cost and poor product quality. Moreover, stakeholders involved in the projects have...

  15. Development of methodologies for virus detection in soybean and wheat seeds.

    Science.gov (United States)

    Botelho, Stephanie R A; Martins, Thais P; Duarte, Macária F; Barbosa, Andreza V; Lau, Douglas; Fernandes, Fernanda R; Sanches, Marcio M

    2016-01-01

    Seeds that contain large amounts of oil, starch, fibers and phenols are the most difficult tissues for RNA extraction. Currently, there are some reports of virus detection in seeds using commercial kits for RNA extraction. However, individual seeds were used, which may not be always suitable for analyses that deal with large amounts of seeds. Sangha [1] described a simple, quick and efficient protocol for RNA extraction and downstream applications in a group of seeds of jatropha (Jatropha curcas), mustard (Brassica sp.) and rice (Oryza sativa). We tested this protocol for soybean (Glycine max), maize (Zea mays), wheat (Triticum aestivum) and triticale (×Triticosecale) seeds and further reverse transcription PCR (RT-PCR)/quantitative real-time PCR (qPCR) in order to have a faster and more practical method for virus detection from seeds than the traditional scheme of seed planting and subsequent Elisa/RT-PCR from leaves. The essential points in the method are:•Some modifications in the protocol [1] were done in order to increase performance: Wheat and triticale seeds are incubated with water prior to maceration. An amount of 1.2 g of dry soybean seeds is used to maceration.•RT-PCR is used for detection of Wheat streak mosaic virus from wheat seeds and RT-qPCR for detection of Soybean mosaic virus from soybean seeds.•The method may be tested for other viruses, however, pre-validation will be needed. PMID:27408831

  16. Development of Groundwater Modeling Support System Based on Service-Oriented Architecture

    Science.gov (United States)

    WANG, Y.; Tsai, J. P.; Hsiao, C. T.; Chang, L. C.

    2014-12-01

    Groundwater simulation has become an essential step on the groundwater resources management and assessment. There are many stand-alone pre and post processing software packages to alleviate the model simulation loading, but the stand-alone software do not consider centralized management of data and simulation results neither do they provide network sharing function. The model buildings are still implemented independently case to case when using these packages. Hence, it is difficult to share and reuse the data and knowledge (simulation cases) systematically within or across companies. Therefore, this study develops a centralized and network based groundwater model developing system to assist model simulation. The system is based on service-oriented architecture and allows remote user to develop their modeling cases on internet. The data and cases (knowledge) are thus easy to manage centralized. MODFLOW is the modeling engine of the system, which is the most popular groundwater model in the world. Other functions include the database management and variety of model developing assisted web services including auto digitalizing of geology profile map、groundwater missing data recovery assisting、graphic data demonstration and auto generation of MODFLOW input files from database that is the most important function of the system. Since the system architecture is service-oriented, it is scalable and flexible. The system can be easily extended to include the scenarios analysis and knowledge management to facilitate the reuse of groundwater modeling knowledge.

  17. Development of the switch requirements and architecture of a safety data communication system

    International Nuclear Information System (INIS)

    In accordance with digitalising the Instrumentation and Control(I and C) systems in the integral reactor, a communication network is required for effective information exchanges between the different equipment, an enhancement of the design flexibility, a simple installation and cost reduction. Generally, a communication network consists of a topology, the protocol, a communication medium, an interconnection device, etc. In this report, the development methods of switch and the architecture of a Safety Data Communication System(SDCS) are investigated and analyzed. In this report, the design requirements for switch are presented, which are the essential requirements to develop the switch in a SDCS of the SMART-P. To establish these requirements, the evaluation and analysis of the design and implementation method of the COTS switches, the architecture of SDCS and the design requirements of a SDCS were performed. At the detail design stage, these requirements will be used for the top-tier requirements, especially the design target and design basis. To develop the detail design requirements in the future, more quantitative and qualitative analyses are required. In the case of selecting the COTS switch and developing the switch, these requirements will also be used for the evaluation guide

  18. Arabidopsis CPR5 independently regulates seed germination and postgermination arrest of development through LOX pathway and ABA signaling.

    Directory of Open Access Journals (Sweden)

    Guilan Gao

    Full Text Available The phytohormone abscisic acid (ABA and the lipoxygenases (LOXs pathway play important roles in seed germination and seedling growth and development. Here, we reported on the functional characterization of Arabidopsis CPR5 in the ABA signaling and LOX pathways. The cpr5 mutant was hypersensitive to ABA in the seed germination, cotyledon greening and root growth, whereas transgenic plants overexpressing CPR5 were insensitive. Genetic analysis demonstrated that CPR5 gene may be located downstream of the ABI1 in the ABA signaling pathway. However, the cpr5 mutant showed an ABA independent drought-resistant phenotype. It was also found that the cpr5 mutant was hypersensitive to NDGA and NDGA treatment aggravated the ABA-induced delay in the seed germination and cotyledon greening. Taken together, these results suggest that the CPR5 plays a regulatory role in the regulation of seed germination and early seedling growth through ABA and LOX pathways independently.

  19. Disruption of germination and seedling development in Brassica napus by mutations causing severe seed hormonal imbalance

    Directory of Open Access Journals (Sweden)

    Tung eNguyen

    2016-03-01

    Full Text Available The Brassica napus (oilseed rape accession 1012-98 shows a disturbed germination phenotype that was thought to be associated with its lack of testa pigmentation and thin seed coat. Here we demonstrate that the disturbed germination and seedling development are actually due to independent mutations that disrupt the balance of hormone metabolites and their regulators in the seeds. High-throughput UPLC-MS/MS hormone profiling of seeds and seedlings before and after germination revealed that 1012-98 has a severely disturbed hormone balance with extremely atypical, excessive quantities of auxin and ABA metabolites. The resulting hypersensitivity to abscisic acid (ABA and a corresponding increase in dormancy often results in death of the embryo after imbibition or high frequencies of disturbed, often lethal developmental phenotypes, resembling Arabidopsis mutants for the auxin regulatory factor gene ARF10 or the auxin-overproducing transgenic line iaaM-OX. Molecular cloning of Brassica ARF10 orthologues revealed four loci in normal B. napus, two derived from the Brassica A genome and two from the C genome. On the other hand, the phenotypic mutant 1012-98 exhibited amplification of C-genome BnaC.ARF10 copy number along with a chimeric allele originating from recombination between homoeologous A and C genome loci which lead to minor increase of Bna.ARF10 transcription on the critical timepoint for seed germination, the indirect regulator of ABI3, the germinative inhibitor. Bna.GH3.5 expression was upregulated to conjugate free auxin to IAA-asp between 2-6 DAS. Functional amino acid changes were also found in important DNA binding domains of one BnaC.ARF10 locus, suggesting that regulatory changes in Bna.ARF10 are collectively responsible for the observed phenotpyes in 1012-98. To our knowledge, this study is the first to report disruption of germination and seedling development in Brassica napus caused by the crosstalk of auxin-ABA and the

  20. Collaborative Solution Architecture for Developing a National Interoperability Framework in Romania

    Directory of Open Access Journals (Sweden)

    Bogdan GHILIC-MICU

    2010-01-01

    Full Text Available Interoperability framework is a set of standards and guidelines that describe how organizations have established or will establish to interact. The framework is not static, but one that adapts to the change of standards, administrative requirements and technology. It can be adapted to the socio - economic, political, cultural, linguistic, historical and geographical purposes and to a specific context or situation. The article aims to clarify the essential concepts necessary for outlining Romanian national interoperability framework and to propose collaborative solution architecture for its development, updating and maintaining.

  1. Architectural Mealscapes

    DEFF Research Database (Denmark)

    Tvedebrink, Tenna Doktor Olsen; Fisker, Anna Marie; Kirkegaard, Poul Henning

    2012-01-01

    German architect Gottfried Semper developed a theory on the “four elements of Architecture” tracing the origin of architecture back to the rise of the early human settlement and the creation of fire. With the notion ‘hearth’ as the first motive in architecture and the definition of three enclosing...... motives; mounding, enclosure and roof, Semper linked the cultural and social values of the primordial fireplace with the order and shape of architecture. He claimed that any building ever made was nothing but a variation of the first primitive shelters erected around the fireplace, and that the three...... enclosing motives existed only as defenders of the “sacred flame”. In that way Semper developed the idea that any architectural scenery can be described, analyzed and explained by understanding the contextual, symbolic and social values of how the four basic motives of hearth, mounding, enclosure, and roof...

  2. State of arbitrary complexity and Model Driven Architecture in the software development in Cuba

    Directory of Open Access Journals (Sweden)

    Nemury Silega

    2014-01-01

    Full Text Available Informatic systems play an important role in the society because are used in the majority of fields. The community of software researchers and developers devotes substantial efforts to determine how software systems can increasingly improve the environment in which they are deployed, for example, in medicine, business management, education, among other domains. But paradoxically these efforts do not correspond to those devoted to improving the environment of software development itself. There are insufficient proposals to streamline the software development process. The authors conducted an analysis of several proposals to improve the software development process in Cuba, propose how to address the problem based on the paradigm of Model Driven Architecture and make an assessment of the impact this may have on Cuban software industry.

  3. The impact of temperature regimes on development, dormancy breaking and germination of dwarf shrub seeds from arctic, alpine and boreal sites

    DEFF Research Database (Denmark)

    Graae, Bente Jessen; Alsos, Inger Greve; Ejrnæs, Rasmus

    2008-01-01

    species. We examined whether recruitment of Empetrum nigrum ssp. hermaphroditum and Vaccinium uliginosum (hereafter E. nigrum and V. uliginosum) was affected by temperature during three reproductive stages: seed development, dormancy breakage and germination. Seeds were collected from an arctic, an alpine...... (only E. nigrum) and a boreal site with different climates; stored at different winter temperatures and incubated for germination at different temperatures. Seeds of V. uliginosum developed in the boreal region had a higher percentage germination than did seeds developed in the Arctic. In contrast......, seeds of E. nigrum from the arctic site had a higher or similar percentage germination than did seeds from the alpine and boreal sites. Increased winter temperatures had no significant effect on resulting germination percentage of E. nigrum. However, V. uliginosum seeds from the arctic site suffered...

  4. Changes in the composition of pumpkin seeds (Cucurbita moschata during development and maturation

    Directory of Open Access Journals (Sweden)

    Petkova, Zh. Y.

    2015-03-01

    Full Text Available Changes in the chemical and lipid composition of Cucurbita moschata seeds and seed oils at different stages of development were investigated. The oil content of the seeds at 30, 60 and 90 days after flowering was 10.7, 41.1, and 47.1%, respectively. The amount of proteins was 26.0, 35.9, and 38.2%. The contents of carbohydrates soluble in ethanol were 9.5, 1.8 and 1.3%. The starch and fiber percentage contents were 16.3, 6.8, 2.3 and 4.0, 6.9 and 10.0, respectively and the ash contents were 7.2, 4.7, and 4.5%. The total sterol percentages were found to be 2.0, 0.8 and 0.6 in the oils and 0.2, 0.3 and 0.3 in the seeds. The tocopherol contents were 2010, 512 and 527 mg·kg−1 in the oil, and 215, 210 and 250 mg·kg−1 in the seeds. The total phospholipid percentages were 8.7, 0.8 and 0.4 in the oils and 0.9, 0.3 and 0.2 in the seeds. Fatty acid composition was determined by gas chromatography and the major fatty acids in the oils at all stages of ripening were linoleic (40.8–50.2% followed by palmitic (21.5–25.9% and oleic (20.5–21.0%.Se determinaron los cambios en la composición química y en los lípidos de semillas de Cucurbita moschata así como en los aceites extraídos en diferentes etapas del desarrollo de las semillas. El contenido de aceite a los 30, 60 y 90 días después de la floración fue de 10,7, 41,1 y 47,1%, respectivamente. La cantidad de proteínas fue del 26,0, 35,9 y 38,2% y el contenido de hidratos de carbono solubles en etanol fue de 9,5, 1,8 y 1,3%. Los contenidos de almidón y fibras fueron 16,3, 6,8, 2,3% y 4,0, 6,9 y 10,0%, respectivamente y el contenido de cenizas fue de 7.2%, 4.7% y 4.5%. Se encontró un contenido total de esteroles del 2,0, 0,8 y 0,6% en los aceites y de 0,2, 0,3 y 0,3% en las semillas. El contenido de tocoferoles fue 2010, 512 y 527 mg·kg−1 en el aceite, y 215, 210 y 250 mg·kg−1 en las semillas. Los contenidos de fosfolípidos totales fueron de 8,7, 0,8 y 0,4% en los aceites y 0,9, 0

  5. Evaluation and development of therapeutic management of subacute toxicity of Jatropha curcas seed oil in goats

    Directory of Open Access Journals (Sweden)

    Amit Shukla

    2013-09-01

    Full Text Available Aim: The present study was designed to evaluate and develop the therapeutic management for toxicity of Jatropha curcasseed oil in goats.Materials and Methods: Sub-acute toxicity of J. curcas seed oil was evaluated by using nine healthy goats, weighing 28-32kg and 16-18 months old, equally and randomly divided in three groups (n = 3 per group. Adequate measures were taken tominimize pain to the animals. Group I served as control; they did not receive any treatment. The study was conducted by dailyoral dosing of J. curcas seed oil @ 1ml / kg body weight followed by the treatment with the therapeutic module comprising ofsodium thiosulphate ( 50mg/ kg bodyweight and glutathione ( 0.25 mg/ kg body weight in group II; whereas, goats in groupIII were given daily oral dosing of J. curcas seed oil only for 28 days. Hematobiochemical and antioxidative parameters asLipid Peroxidase (LPO and Reduced Glutathione (GSH in erythrocytes were estimated at day 0, 14 and 28 days.Results: Mild to moderate diarrhoea, dullness, depression and letharginess were observed in all animals in group III,whereas, mild clinical signs were observed in treatment group II. Asignificant (P<0.05 decrease in Hb , PCV, TEC ,TLC , totalserum protein , albumin and globulin values were observed in group II and III in comparison to the control group I, however,the group II showed increase in the aforesaid parameters in comparison with without group III indicating the therapeuticefficacy of the therapeutic module. Asignificant (P<0.05 increase in serum creatinine, serum urea, cholesterol, AST, ALTandALP were observed in all the groups. Antioxidative parameters in erythrocytes as LPO and GSH increased in group III incomparison to the control and group II.Conclusion: It is concluded from the above study that therapeutic module comprising of sodium thiosulphate ( 50mg/kg IV and glutathione ( 0.25 mg/kg IM in sub-acute study reduces the toxic effects of J. curcas seed oil as indicated by the

  6. OsSRT1 is involved in rice seed development through regulation of starch metabolism gene expression.

    Science.gov (United States)

    Zhang, Hua; Lu, Yue; Zhao, Yu; Zhou, Dao-Xiu

    2016-07-01

    OsSRT1 is a NAD(+)-dependent histone deacetylase, closely related to the human SIRT6 that plays key roles in genome stability and metabolic homeostasis. In this work, we investigated the role of OsSRT1 in rice seed development. Down-regulation of OsSRT1 induced higher expression of Rice Starch Regulator1 (RSR1) and amylases genes in developing seeds, which resulted in a decrease of starch synthesis and an increase of starch degradation, leading to abnormal seed development. ChIP assay showed that OsSRT1 was required to reduce histone H3K9 acetylation on starch metabolism genes and transposons in developing seeds. In addition, OsSRT1 was detected to directly bind to starch metabolism genes such as OsAmy3B, OsAmy3E, OsBmy4, and OsBmy9. Our results suggested that OsSRT1-mediated histone deacetylation is involved in starch accumulation and transposon repression to regulate normal seed development. PMID:27181944

  7. Disruption of endosperm development is a major cause of hybrid seed inviability between Mimulus guttatus and Mimulus nudatus.

    Science.gov (United States)

    Oneal, Elen; Willis, John H; Franks, Robert G

    2016-05-01

    Divergence of developmental mechanisms within populations could lead to hybrid developmental failure, and might be a factor driving speciation in angiosperms. We investigate patterns of endosperm and embryo development in Mimulus guttatus and the closely related, serpentine endemic Mimulus nudatus, and compare them to those of reciprocal hybrid seed. We address whether disruption in hybrid seed development is the primary source of reproductive isolation between these sympatric taxa. M. guttatus and M. nudatus differ in the pattern and timing of endosperm and embryo development. Some hybrid seeds exhibit early disruption of endosperm development and are completely inviable, while others develop relatively normally at first, but later exhibit impaired endosperm proliferation and low germination success. These developmental patterns are reflected in mature hybrid seeds, which are either small and flat (indicating little to no endosperm) or shriveled (indicating reduced endosperm volume). Hybrid seed inviability forms a potent reproductive barrier between M. guttatus and M. nudatus. We shed light on the extent of developmental variation between closely related species within the M. guttatus species complex, an important ecological model system, and provide a partial mechanism for the hybrid barrier between M. guttatus and M. nudatus. PMID:26824345

  8. Software Architecture: Architecture Constraints

    OpenAIRE

    Tibermacine, Chouki

    2014-01-01

    International audience In this chapter, we introduce an additional, yet essential, concept in describing software architectures : architecture constraints. We explain the precise role of these entities and their importance in object-oriented, component-based or service-oriented software engi-neering. We then describe the way in which they are specified and interpreted. An architect can define architecture constraints and then associate them to architectural descriptions to limit their stru...

  9. THE ROLE OF ARCHITECTURAL PROPAEDEUTICS IN THE DEVELOPMENT OF PROFESSIONAL SKILLS OF AN ARCHITECT

    Directory of Open Access Journals (Sweden)

    Kolstet O. A.

    2015-02-01

    Full Text Available Experience of leading architectural schools and faculties of the country on the integration of propaedeutic course with fundamentals of architectural design in early stages of education is described in the article. The technique of the relationship between composition and specific design tasks, implemented in Rostov architectural school is presented here. The schemes of the phased teaching of students of architectural specialties by the example of the first three course works

  10. Multi-Agent and Service Oriented Architectures for Intelligent Tutoring System Development

    OpenAIRE

    Lavendelis, E; Bicāns, J

    2011-01-01

    Traditional modular architecture of Intelligent Tutoring Systems (ITSs) does not provide sufficient modularity of complex ITSs. Distributed technologies like services and agents are used to increase modularity of ITSs by implementing traditional modules as sets of services or agents. The paper describes holonic agent architecture that implements each module as one or more holonic agents. It uses the lessons learned from the multi-agent architecture to propose service oriented ITS architecture.

  11. Dilemma of green and pseudo green architecture based on LEED norms in case of developing countries

    OpenAIRE

    Mohammadjavad Mahdavinejad; Arash Zia; Airya Norouzi Larki; Setareh Ghanavati; Narjes Elmi

    2014-01-01

    Achieving sustainable and eco-friendly architecture is one of the main objectives that humans for creating a better life have made as the ultimate model for all their professional activities. For this reason, moving towards a greener architecture is considered the main goal of the contemporary architecture of our time. The goal of this study is to analyse architectural projects that have been already performed in the Middle East countries in terms of their compatibility with actual concepts o...

  12. Architecture on Architecture

    DEFF Research Database (Denmark)

    Olesen, Karen

    2016-01-01

    how recognising the autonomy of architecture, not as an esoteric concept but as a valid source of information in a pragmatic design practice, may help us overcome the often-proclaimed dichotomy between formal autonomy and a societally committed architecture. It follows that in architectural education...

  13. Rubisco without the Calvin cycle improves the carbon efficiency of developing green seeds.

    Science.gov (United States)

    Schwender, Jörg; Goffman, Fernando; Ohlrogge, John B; Shachar-Hill, Yair

    2004-12-01

    Efficient storage of carbon in seeds is crucial to plant fitness and to agricultural productivity. Oil is a major reserve material in most seeds, and these oils provide the largest source of renewable reduced carbon chains available from nature. However, the conversion of carbohydrate to oil through glycolysis results in the loss of one-third of the carbon as CO2. Here we show that, in developing embryos of Brassica napus L. (oilseed rape), Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) acts without the Calvin cycle and in a previously undescribed metabolic context to increase the efficiency of carbon use during the formation of oil. In comparison with glycolysis, the metabolic conversion we describe provides 20% more acetyl-CoA for fatty-acid synthesis and results in 40% less loss of carbon as CO2. Our conclusions are based on measurements of mass balance, enzyme activity and stable isotope labelling, as well as an analysis of elementary flux modes. PMID:15592419

  14. Breeding response of transcript profiling in developing seeds of Brassica napus

    Directory of Open Access Journals (Sweden)

    Li Xiaodan

    2009-05-01

    Full Text Available Abstract Background The upgrading of rapeseed cultivars has resulted in a substantial improvement in yield and quality in China over the past 30 years. With the selective pressure against fatty acid composition and oil content, high erucic acid- and low oil-content cultivars have been replaced by low erucic acid- and high oil-content cultivars. The high erucic acid cultivar Zhongyou 821 and its descendent, low erucic acid cultivar Zhongshuang 9, are representatives of two generations of the most outstanding Chinese rapeseed cultivars (B. napus developed the past 2 decades. This paper compares the transcriptional profiles of Zhongshuang 9 and Zhongyou 821 for 32 genes that are principally involved in lipid biosynthesis during seed development in order to elucidate how the transcriptional profiles of these genes responded to quality improvement over the past 20 years. Results Comparison of the cultivar Zhongyou 821 with its descendent, Zhongshuang 9, shows that the transcriptional levels of seven of the 32 genes were upregulated by 30% to 109%, including FAD3, ACCase, FAE1, GKTP, Caleosin, GAPDH, and PEPC. Of the 32 genes, 10 (KAS3, β-CT, BcRK6, P450, FatA, Oleosin, FAD6, FatB, α-CT and SUC1 were downregulated by at least 20% and most by 50%. The Napin gene alone accounted for over 75% of total transcription from all 32 genes assessed in both cultivars. Most of the genes showed significant correlation with fatty acid accumulation, but the correlation in ZS9 was significantly different from that in ZY821. Higher KCR2 activity is associated with higher C16:0, C18:0, and C18:2 in both cultivars, lower C22:1 and total fatty acid content in ZY821, and lower 18:1 in ZS9. Conclusion This paper illustrates the response of the transcription levels of 32 genes to breeding in developing rapeseed seeds. Both cultivars showed similar transcription profiles, with the Napin gene predominantly transcribed. Selective pressure for zero erucic acid, low

  15. Developing an Implementation Framework for the Future Internet using Software Defined Networking and the Y-Comm Architecture

    OpenAIRE

    Mapp, Glenford; Sardis, Fragkiskos; Crowcroft, Jon

    2016-01-01

    The Future Internet will provide seamless connectivityvia heterogeneous networks. The Y-Comm Architecture isa reference model that has been developed to build future mobilesystems for heterogeneous environments. However, the emergenceof Software Defined Networking and Network Functional Virtualizationwill allow the implementation of advanced mobilearchitectures such as Y-Comm to be prototyped and exploredin more detail. This paper proposes an implementation modelfor the Y-Comm architecture ba...

  16. The distribution of fruit and seed toxicity during development for eleven neotropical trees and vines in Central Panama.

    Directory of Open Access Journals (Sweden)

    Noelle G Beckman

    Full Text Available Secondary compounds in fruit mediate interactions with natural enemies and seed dispersers, influencing plant survival and species distributions. The functions of secondary metabolites in plant defenses have been well-studied in green tissues, but not in reproductive structures of plants. In this study, the distribution of toxicity within plants was quantified and its influence on seed survival was determined in Central Panama. To investigate patterns of allocation to chemical defenses and shifts in allocation with fruit development, I quantified variation in toxicity between immature and mature fruit and between the seed and pericarp for eleven species. Toxicity of seed and pericarp was compared to leaf toxicity for five species. Toxicity was measured as reduced hyphal growth of two fungal pathogens, Phoma sp. and Fusarium sp., and reduced survivorship of brine shrimp, Artemia franciscana, across a range of concentrations of crude extract. I used these measures of potential toxicity against generalist natural enemies to examine the effect of fruit toxicity on reductions of fruit development and seed survival by vertebrates, invertebrates, and pathogens measured for seven species in a natural enemy removal experiment. The seed or pericarp of all vertebrate- and wind-dispersed species reduced Artemia survivorship and hyphal growth of Fusarium during the immature and mature stages. Only mature fruit of two vertebrate-dispersed species reduced hyphal growth of Phoma. Predispersal seed survival increased with toxicity of immature fruit to Artemia during germination and decreased with toxicity to fungi during fruit development. This study suggests that fruit toxicity against generalist natural enemies may be common in Central Panama. These results support the hypothesis that secondary metabolites in fruit have adaptive value and are important in the evolution of fruit-frugivore interactions.

  17. Architecture and data processing alternatives for Tse computer. Volume 1: Tse logic design concepts and the development of image processing machine architectures

    Science.gov (United States)

    Rickard, D. A.; Bodenheimer, R. E.

    1976-01-01

    Digital computer components which perform two dimensional array logic operations (Tse logic) on binary data arrays are described. The properties of Golay transforms which make them useful in image processing are reviewed, and several architectures for Golay transform processors are presented with emphasis on the skeletonizing algorithm. Conventional logic control units developed for the Golay transform processors are described. One is a unique microprogrammable control unit that uses a microprocessor to control the Tse computer. The remaining control units are based on programmable logic arrays. Performance criteria are established and utilized to compare the various Golay transform machines developed. A critique of Tse logic is presented, and recommendations for additional research are included.

  18. The investment in scent: time-resolved metabolic processes in developing volatile-producing Nigella sativa L. seeds.

    Directory of Open Access Journals (Sweden)

    Wentao Xue

    Full Text Available The interplay of processes in central and specialized metabolisms during seed development of Nigella sativa L. was studied by using a high-throughput metabolomics technology and network-based analysis. Two major metabolic shifts were identified during seed development: the first was characterized by the accumulation of storage lipids (estimated as total fatty acids and N-compounds, and the second by the biosynthesis of volatile organic compounds (VOCs and a 30% average decrease in total fatty acids. Network-based analysis identified coordinated metabolic processes during development and demonstrated the presence of five network communities. Enrichment analysis indicated that different compound classes, such as sugars, amino acids, and fatty acids, are largely separated and over-represented in certain communities. One community displayed several terpenoids and the central metabolites, shikimate derived amino acids, raffinose, xylitol and glycerol-3-phosphate. The latter are related to precursors of the mevalonate-independent pathway for VOC production in the plastid; also plastidial fatty acid 18∶3n-3 abundant in "green" seeds grouped with several major terpenes. The findings highlight the interplay between the components of central metabolism and the VOCs. The developmental regulation of Nigella seed metabolism during seed maturation suggests a substantial re-allocation of carbon from the breakdown of fatty acids and from N-compounds, probably towards the biosynthesis of VOCs.

  19. The Proteome of Seed Development in the Model Legume Lotus japonicus

    DEFF Research Database (Denmark)

    Dam, Svend; Laursen, Brian S.; Ornfelt, Jane H.;

    2009-01-01

    . In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5......, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique...... three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea...

  20. Design, Development and Evaluation of a Pneumatic Seeder for Automatic Planting of Seeds in Cellular Trays

    Directory of Open Access Journals (Sweden)

    E Movahedi

    2016-04-01

    Full Text Available For planting fine seeds in cellular trays, an automatic pneumatic seeder was designed, constructed and evaluated. CATIA software was used to design and analysis the system parts of the seeder. Different parts of the seeder, including vibrating seed hopper, vacuum boom, seed picking nozzles, seed tube, pneumatic system and electronic control unit for automation of the seeder, were designed and constructed. The area of nozzle orifice was used to calculate the required pressure of nozzle tip. The seeder was evaluated using two sizes of trays. Experiments were performed with five replications and the error of planting the seeds in the 105 and 390-cellular trays were 1.9 and 0.46 percent, respectively. The time of planting for 105 and 390 cellular trays reduced from 20 min (for manual seeding to 35 s and from 90 min to 160 s, respectively.

  1. Advances in experimental methods for root system architecture and root development

    Institute of Scientific and Technical Information of China (English)

    Jun-bang Wang; Xiu-juan Zhang; Chu Wu

    2015-01-01

    Plant roots play important roles in acquisition of water and nutrients, storage, anchoring, transport, and symbiosis with soil microorganisms, thus quantitative researches on root developmental processes are essential to understand root functions and root turnover in ecosystems, and at the same time such researches are the most difficult because roots are hidden underground. Therefore, how to investigate efficiently root functions and root dynamics is the core aspect in underground ecology. In this article, we reviewed some experimental methods used in root resear-ches on root development and root system architecture, and summarized the advantages and shortages of these meth-ods. Based on the analyses, we proposed three new ways to more understand root processes: (1) new experimental materials for root development; (2) a new observatory system comprised of multiple components, including many observatory windows installed in field, analysis software, and automatic data transport devices; (3) new techniques used to analyze quantitatively functional roots.

  2. Open architectures for formal reasoning and deductive technologies for software development

    Science.gov (United States)

    Mccarthy, John; Manna, Zohar; Mason, Ian; Pnueli, Amir; Talcott, Carolyn; Waldinger, Richard

    1994-01-01

    The objective of this project is to develop an open architecture for formal reasoning systems. One goal is to provide a framework with a clear semantic basis for specification and instantiation of generic components; construction of complex systems by interconnecting components; and for making incremental improvements and tailoring to specific applications. Another goal is to develop methods for specifying component interfaces and interactions to facilitate use of existing and newly built systems as 'off the shelf' components, thus helping bridge the gap between producers and consumers of reasoning systems. In this report we summarize results in several areas: our data base of reasoning systems; a theory of binding structures; a theory of components of open systems; a framework for specifying components of open reasoning system; and an analysis of the integration of rewriting and linear arithmetic modules in Boyer-Moore using the above framework.

  3. Development of an automation system for iodine-125 brachytherapy seed production by ND:YAG laser welding

    International Nuclear Information System (INIS)

    The aim of this work is to develop an automation system for iodine-125 radioactive seed production by Nd:YAG laser welding, which has been used successfully in low dose rate brachytherapy treatment. This small seed consists of a welded titanium capsule, with 0.8 mm in diameter and 4.5 mm in length, containing iodine-125 adsorbed onto a silver rod. The iodine-125 seeds are implanted into the human prostate to irradiate the tumor for cancer treatment. Nowadays, the Radiation Technology Center, at IPEN-CNEN/SP imports and distributes 36,000 iodine-125 seeds per year, for the clinics and hospitals in the country. However, the Brazilian market potential is now over 8,000 iodine-125 seeds per month. The local production of these iodine-125 radioactive sources became a priority for the Institute, in order to reduce the price and the problems of prostate cancer management. It will permit to spread their use to a larger number of patients in Brazil. On the other hand, the industrial automation plays an important role for iodine-125 seeds in order to increase the productivity, with high quality and assurance, avoiding human factors, implementing and operating with good manufacturing practices. The technology consists of appliance electronic and electro-mechanical parts and components to control machines and processes. The automation system technology for iodine-125 seed production developed in this work was mainly assembled employing a Programmable Logic Controller, a stepper motor, an Nd:YAG laser welding machine and a supervisory. (author)

  4. Development of an automation system for Iodine-125 brachytherapy seed encapsulated by Nd:YAG laser welding

    International Nuclear Information System (INIS)

    The aim of this work is to develop an automation system for iodine-125 radioactive seed production by Nd:YAG laser welding, which has been used successfully in low dose rate (LDR) brachytherapy treatment. This small seed consists of a welded titanium capsule, with 0.8 mm in diameter and 4.5 mm in length, containing iodine-125 adsorbed onto a silver rod. The iodine-125 seeds are implanted into the human prostate to irradiate the tumor for cancer treatment. Nowadays, the Radiation Technology Center, at Institute for Nuclear and Energy Research, Sao Paulo, Brazil (IPEN-CNEN/SP) imports and distributes 36,000 iodine-125 seeds per year, for the clinics and hospitals in the country. However, the Brazilian market potential is now over 8,000 iodine-125 seeds per month. The local production of these iodine-125 radioactive sources became a priority for the Institute, in order to reduce the price and the problems of prostate cancer management. It will permit to spread their use to a larger number of patients in Brazil. On the other hand, the industrial automation plays an important role for iodine-125 seeds in order to increase the productivity, with high quality and assurance, avoiding human factors, implementing and operating with good manufacturing practices (GMP). The technology consists of appliance electronic and electro-mechanical parts and components to control machines and processes. The automation system technology for iodine-125 seed production developed in this work was mainly assembled employing a programmable logic controller (PLC), a stepper motor, an Nd:YAG laser welding machine and a supervisory. The statistical repeatability of correctly encapsulated sealed sources with this automation system is greater than 95%. (authors)

  5. Development of an automation system for iodine-125 brachytherapy seed production by ND:YAG laser welding

    Energy Technology Data Exchange (ETDEWEB)

    Somessari, Samir L.; Feher, Anselmo; Sprenger, Francisco E.; Rostellato, Maria Elisa C.M.; Costa, Fabio E.; Calvo, Wilson A.P., E-mail: somessar@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP) Sao Paulo, SP (Brazil)

    2009-07-01

    The aim of this work is to develop an automation system for iodine-125 radioactive seed production by Nd:YAG laser welding, which has been used successfully in low dose rate brachytherapy treatment. This small seed consists of a welded titanium capsule, with 0.8 mm in diameter and 4.5 mm in length, containing iodine-125 adsorbed onto a silver rod. The iodine-125 seeds are implanted into the human prostate to irradiate the tumor for cancer treatment. Nowadays, the Radiation Technology Center, at IPEN-CNEN/SP imports and distributes 36,000 iodine-125 seeds per year, for the clinics and hospitals in the country. However, the Brazilian market potential is now over 8,000 iodine-125 seeds per month. The local production of these iodine-125 radioactive sources became a priority for the Institute, in order to reduce the price and the problems of prostate cancer management. It will permit to spread their use to a larger number of patients in Brazil. On the other hand, the industrial automation plays an important role for iodine-125 seeds in order to increase the productivity, with high quality and assurance, avoiding human factors, implementing and operating with good manufacturing practices. The technology consists of appliance electronic and electro-mechanical parts and components to control machines and processes. The automation system technology for iodine-125 seed production developed in this work was mainly assembled employing a Programmable Logic Controller, a stepper motor, an Nd:YAG laser welding machine and a supervisory. (author)

  6. Development of an automation system for iodine-125 brachytherapy seed production by (Nd:YAG) laser welding

    International Nuclear Information System (INIS)

    The aim of this work is to develop an automation system for iodine-125 radioactive seed production by (Nd:YAG) laser welding, which has been used successfully in Low Dose Rate (LDR) brachytherapy treatment. This small seed consists of a welded titanium capsule, with 0.8mm in diameter and 4.5mm in length, containing iodine-125 adsorbed onto a silver rod. The iodine-125 seeds are implanted into the human prostate to irradiate the tumor for cancer treatment. Nowadays, the Radiation Technology Center, at IPEN-CNEN/SP imports and distributes 36,000 iodine-125 seeds per year, for the clinics and hospitals in the country. However, the Brazilian market potential is now over 8,000 iodine-125 seeds per month. The local production of these iodine-125 radioactive sources becomes a priority for the Institute, in order to reduce the price and the problems of prostate cancer management. It will permit to spread their use to a largest number of patients in Brazil. On the other hand, the industrial automation plays an important role for iodine-125 seeds in order to increase the productivity, with high quality and assurance, avoiding human factors, implementing and operating with Good Manufacturing Practices (GMP). The technology consists of appliance electronic and electro-mechanical parts and components to control machines and processes. The automation system technology for iodine-125 seed production developed in this work was mainly assembled employing Programmable Logic Controller (PLC), stepper motors, drivers, (Nd:YAG) laser welding machine, photoelectric sensors and supervisory. (author)

  7. Historical development of administration architecture in Malaysia (15th-21st century)

    Science.gov (United States)

    Mohidin, H. H. B.; Ismail, A. S.

    2014-02-01

    The main purpose of this paper is to document the development of the state administration building in Malaysia before and after the independence era, in relation to the evolutionary period of Malaysia's political, social and economic history. Multiple case study approach [19] is applied by referring to six prominent case studies to represent state administrative buildings from various phases of Malaysian history beginning from 14th century to 21st century as exemplar. Since this paper formulates new ways to approach and describes state administrative building design and factors that influence them, it uses interpretivism paradigm and (semiotics) as methodological approach to study the relationship between the building design and contextual elements. This paper, therefore, offers new insights, which not only add to knowledge in this field by widening and strengthening the understanding of state administrative architecture in Malaysia, but also are valuable for range of associated fields including architectural semiotics and non verbal communication. This is because this paper reveals deep understandings of the built form and material environment operating as a sign in a cultural and social context.

  8. Model-Driven Development of Reliable Avionics Architectures for Lunar Surface Systems

    Science.gov (United States)

    Borer, Nicholas; Claypool, Ian; Clark, David; West, John; Somervill, Kevin; Odegard, Ryan; Suzuki, Nantel

    2010-01-01

    This paper discusses a method used for the systematic improvement of NASA s Lunar Surface Systems avionics architectures in the area of reliability and fault-tolerance. This approach utilizes an integrated system model to determine the effects of component failure on the system s ability to provide critical functions. A Markov model of the potential degraded system modes is created to characterize the probability of these degraded modes, and the system model is run for each Markov state to determine its status (operational or system loss). The probabilistic results from the Markov model are first produced from state transition rates based on NASA data for heritage failure rate data of similar components. An additional set of probabilistic results are created from a representative set of failure rates developed for this study, for a variety of component quality grades (space-rated, mil-spec, ruggedized, and commercial). The results show that careful application of redundancy and selected component improvement should result in Lunar Surface Systems architectures that exhibit an appropriate degree of fault-tolerance, reliability, performance, and affordability.

  9. Historical development of administration architecture in Malaysia (15th-21st century)

    International Nuclear Information System (INIS)

    The main purpose of this paper is to document the development of the state administration building in Malaysia before and after the independence era, in relation to the evolutionary period of Malaysia's political, social and economic history. Multiple case study approach [19] is applied by referring to six prominent case studies to represent state administrative buildings from various phases of Malaysian history beginning from 14th century to 21st century as exemplar. Since this paper formulates new ways to approach and describes state administrative building design and factors that influence them, it uses interpretivism paradigm and (semiotics) as methodological approach to study the relationship between the building design and contextual elements. This paper, therefore, offers new insights, which not only add to knowledge in this field by widening and strengthening the understanding of state administrative architecture in Malaysia, but also are valuable for range of associated fields including architectural semiotics and non verbal communication. This is because this paper reveals deep understandings of the built form and material environment operating as a sign in a cultural and social context

  10. SEED GERMINATION AND PLANT DEVELOPMENT IN Escobedia grandiflora (OROBANCHACEAE: EVIDENCE OF OBLIGATE HEMIPARASITISM?

    Directory of Open Access Journals (Sweden)

    Edison CARDONA-MEDINA

    2015-07-01

    Full Text Available Root parasitic plants can be facultative or obligate. Facultative parasites are able to complete their life cycle and their seeds can germinate without a host. Escobedia grandiflora is a poorly studied species in spite of its ancestral importance as dye of foods and medicinal use. The present study evaluates the states of seed, seedlings and mature plants, under presence and absence of possible hosts, for inferring the type of parasitism exhibited by E. grandiflora. Seeds were evaluated using two conditions each of light (12 and 0 hours and temperature (20 ºC and 25 ºC; percentage germination, and germination speed were determined. The seeds did not require a host to germinate, as is typical of facultative parasitic plants.  Percentage of germination varied between 66 % and 85.3 % and was not affected by light or temperature although germination speed was greater at 25 ºC. Larger seeds had a higher percentage of germination and produced larger seedlings. The seedlings planted without a host did not survive, while those planted with Paspalum notatum had a 45 % survival rate, demonstrating that this is a critical stage of development, even with a host. Escobedia grandiflora plants sowed with grasses began the reproductive stage at the 28th week, and those planted with Pennisetum purpureum showed better performance, expressed in more haustoria, higher dry matter of total plant, rhizome and aerial stems. Plants sowed alone lived for more than six months, but they did not produce flowers or fruits. According to the behavior of seedlings and plants, E. grandiflora is an obligate parasite. Germinación de semillas y desarrollo de plantas en Escobedia grandiflora (Orobanchaceae: ¿Evidencia de hemiparasitismo obligado? Las plantas parásitas de raíces pueden ser facultativas u obligadas, las primeras pueden completar su ciclo de vida y sus semillas pueden germinar sin un hospedero. Escobedia grandiflora es una especie poco estudiada, a pesar de

  11. Members of the aquaporin family in the developing pea seed coat include representatives of the PIP, TIP, and NIP subfamilies

    OpenAIRE

    Schuurmans, J.A.M.J.; van Dongen, J. T.; Rutjens, B.P.W.; Boonman, Alex; Pieterse, C. M. J.; Borstlap, A.C.

    2003-01-01

    Water and nutrients required by developing seeds are mainly supplied by the phloem and have to be released from a maternal parenchyma tissue before being utilized by the filial tissues of embryo and endosperm. To identify aquaporins that could be involved in this process four full-length cDNAs were cloned and sequenced from a cDNA library of developing seed coats of pea (Pisum sativum L.). The cDNA of PsPIP1-1 appeared to be identical to that of clone 7a/TRG-31, a turgor-responsive gene clone...

  12. Development of short and early maturing malagkit sungsong rice through seed irradiation

    International Nuclear Information System (INIS)

    Seeds of Malagkit Sungsong rice with moisture content stabilized between 13 and14% were irradiated with gamma rays at 300 and 350 Gy to develop a chort and early maturing glutinous rice variety. The early radiation effects indicated that germination was not affected. However, reduction in percent survival and seedling heights were noted as the dose increased. Striata, virescent and albina were the chlorophyll mutations observed in the M2. Virescent and albina predominated especially in the 350 Gy dose. On the morphological mutations, the highest was in the 350 Gy dose. Most of the mutations were with respect to reduction in height and maturity and in grain shape. No significant differences in yield were observed between the mutants and the original cultivar even as the maturity of the earliest yielding mutant was 14 days earlier than the control. (Author)

  13. Architectures of prototypes and architectural prototyping

    DEFF Research Database (Denmark)

    Hansen, Klaus Marius; Christensen, Michael; Sandvad, Elmer;

    1998-01-01

    sessions with users, - evolve over a long period of time to contain more functionality - allow for 6-7 developers working intensively in parallel. Explicit focus on the software architecture and letting the architecture evolve with the prototype played a major role in resolving these conflicting...... constraints. Specifically allowing explicit restructuring phases when the architecture became problematic showed to be crucial.  ...

  14. Exploring triacylglycerol biosynthetic pathway in developing seeds of Chia (Salvia hispanica L.): a transcriptomic approach.

    Science.gov (United States)

    R V, Sreedhar; Kumari, Priya; Rupwate, Sunny D; Rajasekharan, Ram; Srinivasan, Malathi

    2015-01-01

    Chia (Salvia hispanica L.), a member of the mint family (Lamiaceae), is a rediscovered crop with great importance in health and nutrition and is also the highest known terrestrial plant source of heart-healthy omega-3 fatty acid, alpha linolenic acid (ALA). At present, there is no public genomic information or database available for this crop, hindering research on its genetic improvement through genomics-assisted breeding programs. The first comprehensive analysis of the global transcriptome profile of developing Salvia hispanica L. seeds, with special reference to lipid biosynthesis is presented in this study. RNA from five different stages of seed development was extracted and sequenced separately using the Illumina GAIIx platform. De novo assembly of processed reads in the pooled transcriptome using Trinity yielded 76,014 transcripts. The total transcript length was 66,944,462 bases (66.9 Mb), with an average length of approximately 880 bases. In the molecular functions category of Gene Ontology (GO) terms, ATP binding and nucleotide binding were found to be the most abundant and in the biological processes category, the metabolic process and the regulation of transcription-DNA-dependent and oxidation-reduction process were abundant. From the EuKaryotic Orthologous Groups of proteins (KOG) classification, the major category was "Metabolism" (31.97%), of which the most prominent class was 'carbohydrate metabolism and transport' (5.81% of total KOG classifications) followed by 'secondary metabolite biosynthesis transport and catabolism' (5.34%) and 'lipid metabolism' (4.57%). A majority of the candidate genes involved in lipid biosynthesis and oil accumulation were identified. Furthermore, 5596 simple sequence repeats (SSRs) were identified. The transcriptome data was further validated through confirmative PCR and qRT-PCR for select lipid genes. Our study provides insight into the complex transcriptome and will contribute to further genome-wide research and

  15. Identification and study of the relationship between factors and dimensions affecting the security of developed information systems using service-oriented architecture

    OpenAIRE

    Mandana izadi; Mohammad reza taghva

    2014-01-01

    Certain advantages of service-oriented architecture have led to the spread of this type of architecture around the world. However, some special features of this type of architecture have led to more compromise between the security in the information system and other information systems. The purpose of this paper is to identify and examine the impact of factors and dimensions of the information security system that has developed by using service-oriented architecture. In this study, the explor...

  16. EVALUATING THE DISCONTINUED TRADITIONS OF MALAY WOOD CARVINGS IN MALAYSIA: A FAILURE TO DEVELOP THE DISCOURSE ON MODERN AND POST MODERN ORNAMENTATION IN ARCHITECTURAL WORKS

    OpenAIRE

    Nangkula Utaberta; Mohamad Tajuddin Haji Mohamad Rasdi

    2014-01-01

    The main intention of this study is to contribute some thoughts on why the tradition of wood carvings in traditional Malay architecture was discontinued at the rise of modern and post-modern architectural works in Malaysia. Although the tradition of wood carvings more or less came to a stop in public architectural works with the development of colonial architecture, this study seeks to clarify the modernistic and post-modern arguments on the problem or issues of ornamentation. Although modern...

  17. Priorities in seed pathology research

    OpenAIRE

    Nameth S.T.

    1998-01-01

    Seed pathology as a subdisipline of plant pathology is relatively new. Paul Neergard is considered the father of seed pathology. Recent developments in the area of seed pathology technology allow for more ecofriendly seed treatments and more reliable seed health testing. Due to economics and new interest in environmental issues, research into the viability of biological seed treatments is becoming more common. The use of sophisticated DNA amplification technologies allows for the detection of...

  18. The rice GERMINATION DEFECTIVE 1, encoding a B3 domain transcriptional repressor, regulates seed germination and seedling development by integrating GA and carbohydrate metabolism

    OpenAIRE

    Guo, Xiaoli; Hou, Xiaomei; FANG, JUN; Wei, Piwei; Xu, Bo; Chen, Mingluan; Feng, Yuqi; Chu, Chengcai

    2013-01-01

    It has been shown that seed development is regulated by a network of transcription factors in Arabidopsis including LEC1 (LEAFY COTYLEDON1), L1L (LEC1-like) and the B3 domain factors LEC2, FUS3 (FUSCA3) and ABI3 (ABA-INSENSITIVE3); however, molecular and genetic regulation of seed development in cereals is poorly understood. To understand seed development and seed germination in cereals, a large-scale screen was performed using our T–DNA mutant population, and a mutant germination-defective1 ...

  19. Shotgun label-free quantitative proteomics of developing peanut (Arachis hypogaea L.) seed

    Science.gov (United States)

    Legume seeds and peanuts, in particular, are an inexpensive source of plant proteins and edible oil. Owing to their importance in global food security, it is necessary to understand the genetic, biochemical, and physiological mechanisms controlling seed quality and nutritive attributes. A comprehens...

  20. SEED GERMINATION AND ROOT ELONGATION TOXICITY TESTS IN HAZARDOUS WASTE SITE EVALUATION: METHODS DEVELOPMENT AND APPLICATIONS

    Science.gov (United States)

    Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. n the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure co...

  1. Factors affecting seed germination and protocorm development of Vandopsis gigantea (Lindl.) Pfitz. in vitro

    OpenAIRE

    Thibul, S.; Jantasilp, A.

    2006-01-01

    Seeds of Vandopsis gigantea (Lindl.) Pfitz. germinated with highest percentage of 60.62±7.24% on modified VW (Vacin and Went, 1949) medium supplemented with coconut water under light condition. Light not only caused seed germination percentage significantly higher than in darkness (p

  2. The Wnt Signaling Antagonist Kremen1 is Required for Development of Thymic Architecture

    Directory of Open Access Journals (Sweden)

    Masako Osada

    2006-01-01

    Full Text Available Wnt signaling has been reported to regulate thymocyte proliferation and selection at several stages during T cell ontogeny, as well as the expression of FoxN1 in thymic epithelial cells (TECs. Kremen1 (Krm1 is a negative regulator of the canonical Wnt signaling pathway, and functions together with the secreted Wnt inhibitor Dickkopf (Dkk by competing for the lipoprotein receptor-related protein (LRP-6 co-receptor for Wnts. Here krm1 knockout mice were used to examine krm1 expression in the thymus and its function in thymocyte and TEC development. krm1 expression was detected in both cortical and medullary TEC subsets, as well as in immature thymocyte subsets, beginning at the CD25+CD44+ (DN2 stage and continuing until the CD4+CD8+(DP stage. Neonatal mice show elevated expression of krm1 in all TEC subsets. krm1− / − mice exhibit a severe defect in thymic cortical architecture, including large epithelial free regions. Much of the epithelial component remains at an immature Keratin 5+ (K5 Keratin 8+(K8 stage, with a loss of defined cortical and medullary regions. A TOPFlash assay revealed a 2-fold increase in canonical Wnt signaling in TEC lines derived from krm1− / − mice, when compared with krm1+ / + derived TEC lines. Fluorescence activated cell sorting (FACS analysis of dissociated thymus revealed a reduced frequency of both cortical (BP1+EpCAM+ and medullary (UEA-1+ EpCAMhi epithelial subsets, within the krm1− / − thymus. Surprisingly, no change in thymus size, total thymocyte number or the frequency of thymocyte subsets was detected in krm1− / − mice. However, our data suggest that a loss of Krm1 leads to a severe defect in thymic architecture. Taken together, this study revealed a new role for Krm1 in proper development of thymic epithelium.

  3. Dilemma of green and pseudo green architecture based on LEED norms in case of developing countries

    Directory of Open Access Journals (Sweden)

    Mohammadjavad Mahdavinejad

    2014-12-01

    Full Text Available Achieving sustainable and eco-friendly architecture is one of the main objectives that humans for creating a better life have made as the ultimate model for all their professional activities. For this reason, moving towards a greener architecture is considered the main goal of the contemporary architecture of our time. The goal of this study is to analyse architectural projects that have been already performed in the Middle East countries in terms of their compatibility with actual concepts of sustainability and their required green criteria. Therefore, for the sake of review and study, this paper is intended to discover up to what level the sustainability rating system such as LEED (Leadership in Energy and Environmental Design can be effective in rating contemporary architectural projects. Studies indicate three concepts for analysing contemporary architecture and have found to be descriptive: (1 green, (2 pseudo green and (3 energy-monger. The studies have also shown that some of the projects, although trying to display sustainable architecture concepts in appearance, in reality they turned out not to be sustainable enough. In latter steps, this paper intends to evaluate and examine the effectiveness of the LEED rating system. In evaluating LEED rating system, the results inferred indicate that the system is intended more for programming than actual designing purposes and is not an efficient instrument for analysing architectural design process. Analysis based on this study suggests that, for moving from pseudo green to green architecture, it is necessary to use design-oriented patterns.

  4. Development of an in Vitro System to Simulate the Adsorption of Self-Emulsifying Tea (Camellia oleifera Seed Oil

    Directory of Open Access Journals (Sweden)

    Issara Sramala

    2016-04-01

    Full Text Available In this study, tea (Camellia oleifera seed oil was formulated into self-emulsifying oil formulations (SEOF to enhance the aqueous dispersibility and intestinal retention to achieve higher bioavailability. Self-emulsifying tea seed oils were developed by using different concentrations of lecithin in combination with surfactant blends (Span®80 and Tween®80. The lecithin/surfactant systems were able to provide clear and stable liquid formulations. The SEOF were investigated for physicochemical properties including appearance, emulsion droplets size, PDI and zeta potential. The chemical compositions of tea seed oil and SEOF were compared using GC-MS techniques. In addition, the oil adsorption measurement on artificial membranes was performed using a Franz cell apparatus and colorimetric analysis. The microscopic structure of membranes was observed with scanning electron microscopy (SEM. After aqueous dilution with fed-state simulated gastric fluid (FeSSGF, the droplet size of all SEOF was close to 200 nm with low PDI values and the zeta potential was negative. GC-MS chromatograms revealed that the chemical compositions of SEOF were not significantly different from that of the original tea seed oil. The morphological study showed that only the SEOF could form film layers. The oil droplets were extracted both from membrane treated with tea seed oil and the SEOF in order to evaluate the chemical compositions by GC-MS.

  5. Architectural Anthropology

    DEFF Research Database (Denmark)

    Stender, Marie

    Architecture and anthropology have always had a common focus on dwelling, housing, urban life and spatial organisation. Current developments in both disciplines make it even more relevant to explore their boundaries and overlaps. Architects are inspired by anthropological insights and methods......, while recent material and spatial turns in anthropology have also brought an increasing interest in design, architecture and the built environment. Understanding the relationship between the social and the physical is at the heart of both disciplines, and they can obviously benefit from further...... collaboration: How can qualitative anthropological approaches contribute to contemporary architecture? And just as importantly: What can anthropologists learn from architects’ understanding of spatial and material surroundings? Recent theoretical developments in anthropology stress the role of materials and...

  6. Active Debris Removal (ADR) System Architecture Analysis Tool (SAAT) Prototype for Orbital Debris Stabilization and Removal Architecture Development Project

    Data.gov (United States)

    National Aeronautics and Space Administration — It is critically important that a physics based ADR SAAT prototype be developed that can be refined and used across the community to study and analyze various...

  7. Bt Maize Seed Mixtures for Helicoverpa zea (Lepidoptera: Noctuidae): Larval Movement, Development, and Survival on Non-transgenic Maize.

    Science.gov (United States)

    Burkness, Eric C; Cira, T M; Moser, S E; Hutchison, W D

    2015-12-01

    In 2012 and 2013, field trials were conducted near Rosemount, MN, to assess the movement and development of Helicoverpa zea (Boddie) larvae on non-Bt refuge corn plants within a seed mixture of non-Bt and Bt corn. The Bt corn hybrid expressed three Bt toxins-Cry1Ab, Cry1F, and Vip3A. As the use of seed mixtures for insect resistance management (IRM) continues to be implemented, it is necessary to further characterize how this IRM approach impacts resistance development in ear-feeding Lepidopteran pests. The potential for Bt pollen movement and cross pollination of the non-Bt ears in a seed mixture may lead to Bt toxin exposure to larvae developing on those refuge ears. Larval movement and development by H. zea, feeding on non-Bt refuge plants adjacent to either transgenic Bt or non-Bt plants, were measured to investigate the potential for unintended Bt exposure. Non-Bt plants were infested with H. zea eggs and subplots were destructively sampled twice per week within each treatment to assess larval development, location, and kernel injury. Results indicate that H. zea larval movement between plants is relatively low, ranging from 2-16% of larvae, and occurs mainly after reaching the second instar. Refuge plants in seed mixtures did not produce equivalent numbers of H. zea larvae, kernel injury, and larval development differed as compared with a pure stand of non-Bt plants. This suggests that there may be costs to larvae developing on refuge plants within seed mixtures and additional studies are warranted to define potential impacts. PMID:26318006

  8. Changes in the composition of pumpkin seeds (Cucurbita moschata) during development and maturation

    OpenAIRE

    Petkova, Zh. Y.; Antova, G. A.

    2015-01-01

    Changes in the chemical and lipid composition of Cucurbita moschata seeds and seed oils at different stages of development were investigated. The oil content of the seeds at 30, 60 and 90 days after flowering was 10.7, 41.1, and 47.1%, respectively. The amount of proteins was 26.0, 35.9, and 38.2%. The contents of carbohydrates soluble in ethanol were 9.5, 1.8 and 1.3%. The starch and fiber percentage contents were 16.3, 6.8, 2.3 and 4.0, 6.9 and 10.0, respectively and the ash contents were 7...

  9. Accumulation of long-lived mRNAs associated with germination in embryos during seed development of rice

    OpenAIRE

    Sano, Naoto; Ono, Hanako; Murata, Kazumasa; Yamada, Tetsuya; Hirasawa, Tadashi; Kanekatsu, Motoki

    2015-01-01

    Highlight Long-lived mRNAs stored in dry seed are translated after imbibition for germination. We report accumulation of long-lived mRNAs in developing rice embryos and candidates of the mRNAs required for germination.

  10. Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine.

    Science.gov (United States)

    Lee, Siwon; Shin, Yong-Gil

    2014-06-01

    Among imported plants, seeds are the items that have many latent pathogens and are difficult to inspect. Also, they are the import and export items whose market is expected to expand. The biggest problem with seeds is viruses. Prune dwarf virus (PDV) is the virus that is commonly inspected in Prunus cerasifera, P. persica, P. armeniaca, P. mandshurica, P. cerasus, P. avium or P. serotina seeds. In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The method developed in this study has detected PDV in 18 cases since 2007, and is expected to continuously contribute to the plant quarantine in Korea. PMID:25289000

  11. Assessing climate change effects on long-term forest development: adjusting growth, phenology, and seed production in a gap model

    NARCIS (Netherlands)

    Meer, van der P.J.; Jorritsma, I.T.M.; Kramer, K.

    2002-01-01

    The sensitivity of forest development to climate change is assessed using a gap model. Process descriptions in the gap model of growth, phenology, and seed production were adjusted for climate change effects using a detailed process-based growth modeland a regression analysis. Simulation runs over 4

  12. Seed systems support in Kenya

    OpenAIRE

    Munyi, Peter; Jonge, de, M.J.I.

    2015-01-01

    The threats of climate change and rising food prices have stirred renewed attention for seed and food security in Africa, inviting new thinking on the role of seed sector development in coping with these concerns. One conceptual framework that has gained attention is the Integrated Seed Sector Development (ISSD) approach. The ISSD approach has evolved as a response to the almost exclusive focus on formal seed systems in seed sector development programs. Instead, ISSD aims to recognize and sup...

  13. Development of on-line sorting system for detection of infected seed potatoes using visible near-infrared transmittance spectral technique

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae Yong; Cho, Byoung Kwan [Dept. of Biosystems Engineering, Chungnam National University, Daejeon (Korea, Republic of); Mo, Chang Yeun [Rural Development Administration, National Institute of Agricultural Engineering, Jeonju (Korea, Republic of); Kang, Jun Soon [Dept. of Horticultural Bioscience, Pusan National University, Pusan (Korea, Republic of)

    2015-02-15

    In this study, an online seed potato sorting system using a visible and near infrared (40 1100 nm) transmittance spectral technique and statistical model was evaluated for the nondestructive determination of infected and sound seed potatoes. Seed potatoes that had been artificially infected with Pectobacterium atrosepticum, which is known to cause a soil borne disease infection, were prepared for the experiments. After acquiring transmittance spectra from sound and infected seed potatoes, a determination algorithm for detecting infected seed potatoes was developed using the partial least square discriminant analysis method. The coefficient of determination(R{sup 2}{sub p}) of the prediction model was 0.943, and the classification accuracy was above 99% (n = 80) for discriminating diseased seed potatoes from sound ones. This online sorting system has good potential for developing a technique to detect agricultural products that are infected and contaminated by pathogens.

  14. Development of on-line sorting system for detection of infected seed potatoes using visible near-infrared transmittance spectral technique

    International Nuclear Information System (INIS)

    In this study, an online seed potato sorting system using a visible and near infrared (40 1100 nm) transmittance spectral technique and statistical model was evaluated for the nondestructive determination of infected and sound seed potatoes. Seed potatoes that had been artificially infected with Pectobacterium atrosepticum, which is known to cause a soil borne disease infection, were prepared for the experiments. After acquiring transmittance spectra from sound and infected seed potatoes, a determination algorithm for detecting infected seed potatoes was developed using the partial least square discriminant analysis method. The coefficient of determination(R2p) of the prediction model was 0.943, and the classification accuracy was above 99% (n = 80) for discriminating diseased seed potatoes from sound ones. This online sorting system has good potential for developing a technique to detect agricultural products that are infected and contaminated by pathogens.

  15. Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticus infection

    Directory of Open Access Journals (Sweden)

    Liang Xuanqiang

    2008-02-01

    Full Text Available Abstract Background Peanut (Arachis hypogaea L. is an important crop economically and nutritionally, and is one of the most susceptible host crops to colonization of Aspergillus parasiticus and subsequent aflatoxin contamination. Knowledge from molecular genetic studies could help to devise strategies in alleviating this problem; however, few peanut DNA sequences are available in the public database. In order to understand the molecular basis of host resistance to aflatoxin contamination, a large-scale project was conducted to generate expressed sequence tags (ESTs from developing seeds to identify resistance-related genes involved in defense response against Aspergillus infection and subsequent aflatoxin contamination. Results We constructed six different cDNA libraries derived from developing peanut seeds at three reproduction stages (R5, R6 and R7 from a resistant and a susceptible cultivated peanut genotypes, 'Tifrunner' (susceptible to Aspergillus infection with higher aflatoxin contamination and resistant to TSWV and 'GT-C20' (resistant to Aspergillus with reduced aflatoxin contamination and susceptible to TSWV. The developing peanut seed tissues were challenged by A. parasiticus and drought stress in the field. A total of 24,192 randomly selected cDNA clones from six libraries were sequenced. After removing vector sequences and quality trimming, 21,777 high-quality EST sequences were generated. Sequence clustering and assembling resulted in 8,689 unique EST sequences with 1,741 tentative consensus EST sequences (TCs and 6,948 singleton ESTs. Functional classification was performed according to MIPS functional catalogue criteria. The unique EST sequences were divided into twenty-two categories. A similarity search against the non-redundant protein database available from NCBI indicated that 84.78% of total ESTs showed significant similarity to known proteins, of which 165 genes had been previously reported in peanuts. There were

  16. Soil Seed Bank and Plant Community Development in Passive Restoration of Degraded Sandy Grasslands

    Directory of Open Access Journals (Sweden)

    Renhui Miao

    2016-06-01

    Full Text Available To evaluate the efficacy of passive restoration on soil seed bank and vegetation recovery, we measured the species composition and density of the soil seed bank, as well as the species composition, density, coverage, and height of the extant vegetation in sites passively restored for 0, 4, 7, and 12 years (S0, S4, S7, and S12 in a degraded grassland in desert land. Compared with S0, three more species in the soil seed bank at depths of 0–30 cm and one more plant species in the community was detected in S12. Seed density within the topsoil (0–5 cm was five times higher in S12 than that in S0. Plant densities in S7 and S12 were triple and quadruple than that in S0. Plant coverage was increased by 1.5 times (S4, double (S7, and triple (S12 compared with S0. Sørensen’s index of similarity in species composition between the soil seed bank and the plant community were high (0.43–0.63, but it was lower in short-term restoration sites (S4 and S7 than that in no and long-term restoration sites (S0 and S12. The soil seed bank recovered more slowly than the plant community under passive restoration. Passive restoration is a useful method to recover the soil seed bank and vegetation in degraded grasslands.

  17. Looking back on 10 years of the ATLAS Metadata Interface. Reflections on architecture, code design and development methods

    International Nuclear Information System (INIS)

    The 'ATLAS Metadata Interface' framework (AMI) has been developed in the context of ATLAS, one of the largest scientific collaborations. AMI can be considered to be a mature application, since its basic architecture has been maintained for over 10 years. In this paper we describe briefly the architecture and the main uses of the framework within the experiment (TagCollector for release management and Dataset Discovery). These two applications, which share almost 2000 registered users, are superficially quite different, however much of the code is shared and they have been developed and maintained over a decade almost completely by the same team of 3 people. We discuss how the architectural principles established at the beginning of the project have allowed us to continue both to integrate the new technologies and to respond to the new metadata use cases which inevitably appear over such a time period.

  18. Interplay between requirements, software architecture, and hardware constraints in the development of a home control user interface

    DEFF Research Database (Denmark)

    Loft, M.S.; Nielsen, S.S.; Nørskov, Kim;

    2012-01-01

    We have developed a new graphical user interface for a home control device for a large industrial customer. In this industrial case study, we first present our approaches to requirements engineering and to software architecture; we also describe the given hardware platform. Then we make two...... contributions. Our first contribution is to provide a specific example of a real-world project in which a Twin Peaks-compliant approach to software development has been used, and to describe and discuss three examples of interplay between requirements and software architecture decisions. Our second contribution...... is to propose the hardware platform as a third Twin Peaks element that must be given attention in projects such as the one described in this paper. Specifically, we discuss how the presence of severe hardware constraints exacerbates making trade-offs between requirements and architecture....

  19. A Comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes

    OpenAIRE

    Jeong, Claire G.; Hollister, Scott J.

    2010-01-01

    The goal of this study was to determine material effects on cartilage regeneration for scaffolds with the same controlled architecture. The 3D polycaprolactone (PCL), poly (glycerol sebacate) (PGS), and poly (1,8 octanediol-co-citrate) (POC) scaffolds of the same design were physically characterized and tissue regeneration in terms of cell phenotype, cellular proliferation and differentiation, and matrix production were compared to find which material would be most optimal for cartilage regen...

  20. Architecture on Architecture

    DEFF Research Database (Denmark)

    Olesen, Karen

    2016-01-01

    This paper will discuss the challenges faced by architectural education today. It takes as its starting point the double commitment of any school of architecture: on the one hand the task of preserving the particular knowledge that belongs to the discipline of architecture, and on the other hand...... the obligation to prepare students to perform in a profession that is largely defined by forces outside that discipline. It will be proposed that the autonomy of architecture can be understood as a unique kind of information: as architecture’s self-reliance or knowledge-about itself. A knowledge that...... is not scientific or academic but is more like a latent body of data that we find embedded in existing works of architecture. This information, it is argued, is not limited by the historical context of the work. It can be thought of as a virtual capacity – a reservoir of spatial configurations that...

  1. Leveraging Software Architectures to Guide and Verify the Development of Sense/Compute/Control Applications

    CERN Document Server

    Cassou, Damien; Consel, Charles; Lawall, Julia

    2011-01-01

    A software architecture describes the structure of a computing system by specifying software components and their interactions. Mapping a software architecture to an implementation is a well known challenge. A key element of this mapping is the architecture's description of the data and control-flow interactions between components. The characterization of these interactions can be rather abstract or very concrete, providing more or less implementation guidance, programming support, and static verification. In this paper, we explore one point in the design space between abstract and concrete component interaction specifications. We introduce a notion of behavioral contract that expresses the set of allowed interactions between components, describing both data and control-flow constraints. This declaration is part of the architecture description, allows generation of extensive programming support, and enables various verifications. We instantiate our approach in an architecture description language for the doma...

  2. Avionics Architecture for Exploration Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Avionics Architectures for Exploration Project team will develop a system level environment and architecture that will accommodate equipment from multiple...

  3. Architectural geometry

    KAUST Repository

    Pottmann, Helmut

    2014-11-26

    Around 2005 it became apparent in the geometry processing community that freeform architecture contains many problems of a geometric nature to be solved, and many opportunities for optimization which however require geometric understanding. This area of research, which has been called architectural geometry, meanwhile contains a great wealth of individual contributions which are relevant in various fields. For mathematicians, the relation to discrete differential geometry is significant, in particular the integrable system viewpoint. Besides, new application contexts have become available for quite some old-established concepts. Regarding graphics and geometry processing, architectural geometry yields interesting new questions but also new objects, e.g. replacing meshes by other combinatorial arrangements. Numerical optimization plays a major role but in itself would be powerless without geometric understanding. Summing up, architectural geometry has become a rewarding field of study. We here survey the main directions which have been pursued, we show real projects where geometric considerations have played a role, and we outline open problems which we think are significant for the future development of both theory and practice of architectural geometry.

  4. Architectural Anthropology

    DEFF Research Database (Denmark)

    Stender, Marie

    and other spaces that architects are preoccupied with. On the other hand, the distinction between architecture and design is not merely one of scale. Design and architecture represent – at least in Denmark – also quite different disciplinary traditions and methods. Where designers develop prototypes......, architects tend to work with models and plans that are not easily understood by lay people. Further, many architects are themselves sceptical towards notions of user-involvement and collaborative design. They fear that the imagination of citizens and users is restricted to what they are already familiar with...

  5. Development of architecture for digital I and C system using C4ISR framework

    International Nuclear Information System (INIS)

    The architect framework for the digital I and C system is presented in this work. With rapid changes in digital I and C technology, there is a strong need to provide uniform methods to describe the system functions and their performance in context with the physical configuration and logical behavior. C4ISR framework would provide the process and method for the digital system in that it allows the three different views of operational, systems and services, and technical standards. Therefore, stakeholders can share information that is related to the system interfaces, the actions or activities that those components perform, and rules or constraints for those activities from the initial state of system development. As a result, the lifecycle cost and development time for the digital I and C system can also be optimized. These benefits can be obtained by introducing views and products to reveal the logical, behavioral, and performance characteristics of the architecture. To prove this approach, the plant protection system (PPS) is chosen and the architect framework is developed. (author)

  6. A METHOD FOR THE DEVELOPMENT OF PLATFORM MODELS IN THE MODEL DRIVEN ARCHITECTURE CONTEXT

    Directory of Open Access Journals (Sweden)

    Paulo Cezar Stadzisz

    2012-01-01

    Full Text Available The application of the Model Driven Architecture (MDA approach to the design of embedded software based on Real-Time Operating Systems (RTOS is encouraged by the fact that such software has a wide variety of platforms. In this way, the creation of methods for the development of platform models that meet such diversity of platforms is necessary. This study proposes a method called PM-MDA for the development of platform models that design embedded software based on RTOS in the context of MDA. In addition, this study defines the swxRTOS metamodel, a UML 2.0 profile for RTOS based on embedded software design. Such profile defines a set of stereotypes to describe Platform Models (PMs and is intended to generically describe the services provided by a given embedded system platform of the RTOS. This profile promotes the creation of Platform Models, which will be used as input parameters in the model transformation. Due to the inherent complexity in embedded software design and the existence of a wide variety of platform models, new methods that support the development of such software become crucial.

  7. DAsHER CD: Developing a Data-Oriented Human-Centric Enterprise Architecture for EarthCube

    Science.gov (United States)

    Yang, C. P.; Yu, M.; Sun, M.; Qin, H.; Robinson, E.

    2015-12-01

    One of the biggest challenges that face Earth scientists is the resource discovery, access, and sharing in a desired fashion. EarthCube is targeted to enable geoscientists to address the challenges by fostering community-governed efforts that develop a common cyberinfrastructure for the purpose of collecting, accessing, analyzing, sharing and visualizing all forms of data and related resources, through the use of advanced technological and computational capabilities. Here we design an Enterprise Architecture (EA) for EarthCube to facilitate the knowledge management, communication and human collaboration in pursuit of the unprecedented data sharing across the geosciences. The design results will provide EarthCube a reference framework for developing geoscience cyberinfrastructure collaborated by different stakeholders, and identifying topics which should invoke high interest in the community. The development of this EarthCube EA framework leverages popular frameworks, such as Zachman, Gartner, DoDAF, and FEAF. The science driver of this design is the needs from EarthCube community, including the analyzed user requirements from EarthCube End User Workshop reports and EarthCube working group roadmaps, and feedbacks or comments from scientists obtained by organizing workshops. The final product of this Enterprise Architecture is a four-volume reference document: 1) Volume one is this document and comprises an executive summary of the EarthCube architecture, serving as an overview in the initial phases of architecture development; 2) Volume two is the major body of the design product. It outlines all the architectural design components or viewpoints; 3) Volume three provides taxonomy of the EarthCube enterprise augmented with semantics relations; 4) Volume four describes an example of utilizing this architecture for a geoscience project.

  8. Architectural Narratives

    DEFF Research Database (Denmark)

    Kiib, Hans

    2010-01-01

    In this essay, I focus on the combination of programs and the architecture of cultural projects that have emerged within the last few years. These projects are characterized as “hybrid cultural projects,” because they intend to combine experience with entertainment, play, and learning. This essay...... identifies new rationales related to this development, and it argues that “cultural planning” has increasingly shifted its focus from a cultural institutional approach to a more market-oriented strategy that integrates art and business. The role of architecture has changed, too. It not only provides a...... functional framework for these concepts, but tries increasingly to endow the main idea of the cultural project with a spatially aesthetic expression - a shift towards “experience architecture.” A great number of these projects typically recycle and reinterpret narratives related to historical buildings and...

  9. Development of an automatic seed marker registration algorithm using CT and kV X-ray images

    International Nuclear Information System (INIS)

    The purpose of this study is to develop a practical method for determining accurate marker positions for prostate cancer radiotherapy using CT images and kV x-ray images obtained from the use of the on-board imager (OBI). Three gold seed markers were implanted into the reference position inside a prostate gland by a urologist. Multiple digital image processing techniques were used to determine seed marker position and the center-of-mass (COM) technique was employed to determine a representative reference seed marker position. A setup discrepancy can be estimated by comparing a computed COMOBI with the reference COMCT. A proposed algorithm was applied to a seed phantom and to four prostate cancer patients with seed implants treated in our clinic. In the phantom study, the calculated COMCT and COMOBI agree with COMactual within a millimeter. The algorithm also could localize each seed marker correctly and calculated COMCT and COMOBI for all CT and kV x-ray image sets, respectively. Discrepancies of setup errors between 2D-2D matching results using the OBI application and results using the proposed algorithm were less than one millimeter for each axis. The setup error of each patient was in the range of 0.1 ± 2.7 ∼ 1.8 ± 6.6 mm in the AP direction, 0.8 ± 1.6 ∼ 2.0 ± 2.7 mm in the SI direction and -0.9 ± 1.5 ∼ 2.8 ± 3.0 mm in the lateral direction, even though the setup error was quite patient dependent. As it took less than 10 seconds to evaluate a setup discrepancy, it can be helpful to reduce the setup correction time while minimizing subjective factors that may be user dependent. However, the on-line correction process should be integrated into the treatment machine control system for a more reliable procedure

  10. Exploring triacylglycerol biosynthetic pathway in developing seeds of Chia (Salvia hispanica L.: a transcriptomic approach.

    Directory of Open Access Journals (Sweden)

    Sreedhar R V

    Full Text Available Chia (Salvia hispanica L., a member of the mint family (Lamiaceae, is a rediscovered crop with great importance in health and nutrition and is also the highest known terrestrial plant source of heart-healthy omega-3 fatty acid, alpha linolenic acid (ALA. At present, there is no public genomic information or database available for this crop, hindering research on its genetic improvement through genomics-assisted breeding programs. The first comprehensive analysis of the global transcriptome profile of developing Salvia hispanica L. seeds, with special reference to lipid biosynthesis is presented in this study. RNA from five different stages of seed development was extracted and sequenced separately using the Illumina GAIIx platform. De novo assembly of processed reads in the pooled transcriptome using Trinity yielded 76,014 transcripts. The total transcript length was 66,944,462 bases (66.9 Mb, with an average length of approximately 880 bases. In the molecular functions category of Gene Ontology (GO terms, ATP binding and nucleotide binding were found to be the most abundant and in the biological processes category, the metabolic process and the regulation of transcription-DNA-dependent and oxidation-reduction process were abundant. From the EuKaryotic Orthologous Groups of proteins (KOG classification, the major category was "Metabolism" (31.97%, of which the most prominent class was 'carbohydrate metabolism and transport' (5.81% of total KOG classifications followed by 'secondary metabolite biosynthesis transport and catabolism' (5.34% and 'lipid metabolism' (4.57%. A majority of the candidate genes involved in lipid biosynthesis and oil accumulation were identified. Furthermore, 5596 simple sequence repeats (SSRs were identified. The transcriptome data was further validated through confirmative PCR and qRT-PCR for select lipid genes. Our study provides insight into the complex transcriptome and will contribute to further genome-wide research

  11. Agronomic Importance of First Development of Chickpea (Cicer arietinum L. Under Semi-arid Conditions: II. Seed Imbibition

    Directory of Open Access Journals (Sweden)

    A. Oksel

    2012-01-01

    Full Text Available Due to the slowness growth and weakness of the first developments of chickpea (Cicer arietinum L., it could not combated with weeds and easiliy caught up by Ascochyta blight (Ascochyta rabiei (Pass Labr. disease. Additionally, due to biotic and abiotic stress factors, esp. at the late sowing, important seed yield losses could be happened. To be able to avoid from them is only possible to accelerate of its first development as possible as. So, one of the best solutions to is to use chemical compounds such as Humic Acid (HA known soil regulator under the semi-arid conditions. With this aim this research was performed in a Randomized Complete Block Design (RCBD with four replications under semi-arid field conditions during (2008/2009 and (2009/2010 in Turkiye. Two cultivars (V1 = Gokce and V2 = Ispanyol and four seed imbibition methods (A0 = 0, A1 = Tap Water, A2 = ½ Tap Water + ½ Humic acid (HA, A3 = Full HA, as w/w and seven yield components Plant Height (PH, Number of Branches per Plant (NBP, Number of Pods per Plant (NPP, First Pod Height (NFP, Number of Seeds per Pod (NSP, Seed Weight per Plant (SWP and 100-Seed weight (HSW were investigated. The PH and FPH were affected the A0, the NBP, NPP and NSP were affected the A2 and the SWP and HSW were given the varied but not clear responses according to varieties for all the parameters in A1. The A0 and A1 were encouraged the germination and top soil of the plant but, the A2 to A3 were encouraged root system’s development. It was concluded that the A2 is a promising method which makes the maximum and positive effect to the first development of the chickpea agronomy under the semi-arid conditions.

  12. Agronomic importance of first development of chickpea (Cicer arietinum L.) under semi-arid conditions: II. Seed imbibition.

    Science.gov (United States)

    Ulukan, H; Bayraktar, N; Oksel, A; Gursoy, M; Kocak, N

    2012-02-15

    Due to the slowness growth and weakness of the first developments of chickpea (Cicer arietinum L.), it could not combated with weeds and easily caught up by Ascochyta blight (Ascochyta rabiei (Pass) Labr.) disease. Additionally, due to biotic and abiotic stress factors, esp. at the late sowing, important seed yield losses could be happened. To be able to avoid from them is only possible to accelerate of its first development as possible as. So, one of the best solutions to is to use chemical compounds such as Humic Acid (HA) known soil regulator under the semi-arid conditions. With this aim this research was performed in a Randomized Complete Block Design (RCBD) with four replications under semi-arid field conditions during (2008/2009) and (2009/2010) in Turkiye. Two cultivars (V1 = Gokce and V2 = Ispanyol) and four seed imbibition methods (A0 = 0, A1 = Tap Water, A2 = 1/2 Tap Water + 1/2 Humic acid (HA), A3 = Full HA, as w/w) and seven yield components Plant Height (PH), Number of Branches per Plant (NBP), Number of Pods per Plant (NPP), First Pod Height (NFP), Number of Seeds per Pod (NSP), Seed Weight per Plant (SWP) and 100-Seed weight (HSW) were investigated. The PH and FPH were affected the A0, the NBP, NPP and NSP were affected the A2 and the SWP and HSW were given the varied but not clear responses according to varieties for all the parameters in A1. The A0 and A1 were encouraged the germination and top soil of the plant but, the A2 to A3 were encouraged root system's development. It was concluded that the A2 is a promising method which makes the maximum and positive effect to the first development of the chickpea agronomy under the semi-arid conditions. PMID:22816177

  13. Data-Centric Enterprise Architecture

    OpenAIRE

    Zeinab Rajabi; Maryam Nooraei Abade

    2012-01-01

    Enterprises choose Enterprise Architecture (EA) solution, in order to overcome dynamic business challenges and in coordinate various enterprise elements. In this article, a solution is suggested for the Enterprise Architecture development. The solution focuses on architecture data in the Enterprise Architecture development process. Data-centric architecture approach is preferred product-centric architecture approach. We suggest using Enterprise Ontology (EO) as context for collecting architec...

  14. Dormancy Genes From Weedy Rice Respond Divergently to Seed Development Environments

    OpenAIRE

    Gu, Xing-You; Kianian, Shahryar F.; Foley, Michael E.

    2006-01-01

    Genes interacting with seed developmental environments control primary dormancy. To understand how a multigenic system evolved to adapt to the changing environments in weedy rice, we evaluated genetic components of three dormancy QTL in a synchronized nondormant genetic background. Two genetically identical populations segregating for qSD1, qSD7-1, and qSD12 were grown under greenhouse and natural conditions differing in temperature, relative humidity, and light intensity during seed developm...

  15. Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells

    OpenAIRE

    Alain Carpentier; Juan C. Chachques; Fabien Legrand; Samira Benadda; Nermine Lila; Kanwal Haneef

    2012-01-01

    Electrostimulation (ES) can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs) seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocytes. BMSCs were isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring 25 x 25 x 6 mm. Bipolar in vitro ES was performed during 21 days. Electrical im...

  16. A step towards developing adaptive robot-mediated intervention architecture (ARIA) for children with autism.

    Science.gov (United States)

    Bekele, Esubalew T; Lahiri, Uttama; Swanson, Amy R; Crittendon, Julie A; Warren, Zachary E; Sarkar, Nilanjan

    2013-03-01

    Emerging technology, especially robotic technology, has been shown to be appealing to children with autism spectrum disorders (ASD). Such interest may be leveraged to provide repeatable, accurate and individualized intervention services to young children with ASD based on quantitative metrics. However, existing robot-mediated systems tend to have limited adaptive capability that may impact individualization. Our current work seeks to bridge this gap by developing an adaptive and individualized robot-mediated technology for children with ASD. The system is composed of a humanoid robot with its vision augmented by a network of cameras for real-time head tracking using a distributed architecture. Based on the cues from the child's head movement, the robot intelligently adapts itself in an individualized manner to generate prompts and reinforcements with potential to promote skills in the ASD core deficit area of early social orienting. The system was validated for feasibility, accuracy, and performance. Results from a pilot usability study involving six children with ASD and a control group of six typically developing (TD) children are presented. PMID:23221831

  17. Design and development of annatto (Bixa orellana L.) seed separator machine.

    Science.gov (United States)

    Math, R G; Ramesh, G; Nagender, A; Satyanarayana, A

    2016-01-01

    A small, continuous power operated machine with a capacity of 132 kg/h was designed, fabricated and evaluated its performance for separation of annatto (Bixa orellana L.) seeds from freshly harvested pods. Physical and engineering parameters of annatto pods and seeds were evaluated to design a machine. The densities of pod, seed, and husk are 134.7 to 186.0 kg/m(3), 1206 to 1253.9 kg/m(3), 317 to 381.4 kg/m(3) respectively. The moisture content of seeds were in the range 18.83 - 20.1 % (db) and pod 8 to 10 % (db). Bixin content of seed was in the range 1.34 to 1.765 %. The friction angle for pod, seed, and husk lies between angle 30.15° to 35.76°. Breaking force was 98 N at a span of 14.5 mm. The machine consisted of concentric cylinders and beaters with different pitch of 40, 45 and 50 mm and a length of shaft is 800 mm. The actual machine output was observed to be 132.5 kg/h. Maximum bixin content was found to be retained at 300 rpm. PMID:26787990

  18. Seed quality in informal seed systems

    NARCIS (Netherlands)

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural producti

  19. Leveraging software architectures to guide and verify the development of sense/compute/control applications

    DEFF Research Database (Denmark)

    Cassou, Damien; Balland, Emilie; Consel, Charles;

    2011-01-01

    A software architecture describes the structure of a computing system by specifying software components and their interactions. Mapping a software architecture to an implementation is a well known challenge. A key element of this mapping is the architecture’s description of the data and control......-flow interactions between components. The characterization of these interactions can be rather abstract or very concrete, providing more or less implementation guidance, programming support, and static verification. In this paper, we explore one point in the design space between abstract and concrete component...... verifications. We instantiate our approach in an architecture description language for Sense/Compute/Control applications, and describe associated compilation and verification strategies....

  20. Cattle and sheep develop preference for drinking water containing grape seed tannin.

    Science.gov (United States)

    Kronberg, S L; Schauer, C S

    2013-10-01

    Ingestion of small amounts of some types of condensed tannins (CTs) by ruminant livestock can provide nutritional, environmental and economic benefits. However, practical methods are needed to make these tannins more available to ruminant livestock. Results from previous trials with crude quebracho and black wattle tannin indicated that cattle and/or sheep would not preferentially drink water containing these tannins. Therefore, we conducted preference trials to determine if cattle and sheep would learn to prefer water containing purified grape seed tannin (GST) that provided up to 2% of their daily dry matter (DM) intake. After gradual exposure to increasing amounts of this tannin in water during a pre-trial period, five adult ewes and five yearling heifers fed lucerne (Medicago sativa) pellets (19% CP) were offered water and several concentrations of GST solutions for either 15 (sheep trial) or 20 days (cattle trial). We measured intake of all liquids daily. Concentrations of blood urea were also measured for heifers when they drank only tannin solutions or water. Both sheep and cattle developed preferences for water with GST in it over water alone (P < 0.01) although this preference appeared earlier in the trial for sheep than for cattle. For the sheep, mean daily intake of water alone and all tannin solutions (in total) was 0.6 and 6.1 l, respectively. For the cattle, mean daily intake of water and all tannin solutions in total was 21.8 and 20.6 l, respectively, in the first half of the trial and 10.8 and 26.1 l, respectively, in the second half of the trial. Compared with the other tannin solutions, both sheep and cattle drank more of the solution with the highest tannin concentration (2% of daily DM intake as GST) than of water on more trial days (P < 0.05). Ingestion of water with the highest concentration of GST reduced blood plasma urea concentration in the cattle by 9% to 14% (P ≤ 0.10) compared with ingestion of water alone. Results from the trials

  1. Differentially expressed microRNA cohorts in seed development may contribute to poor grain filling of inferior spikelets in rice

    OpenAIRE

    Peng, Ting; Sun, Hongzheng; Qiao, Mengmeng; Zhao, Yafan; Du, Yanxiu; Zhang, Jing; Li, Junzhou; Tang, Guiliang; Zhao, Quanzhi

    2014-01-01

    Background The inferior spikelets are defined to be those at portions where the grains receive less photosynthetic products during the seed development. The typical inferior spikelets are physically located on the proximal secondary branches in a rice panicle and traditionally characterized by a later flowering time and a slower grain-filling rate, compared to those so-called superior spikelets. Grains produced on the inferior spikelets are consequently under-developed and lighter in weight t...

  2. CvADH1, a member of short-chain alcohol dehydrogenase family, is inducible by gibberellin and sucrose in developing watermelon seeds.

    Science.gov (United States)

    Kim, Joonyul; Kang, Hong-Gyu; Jun, Sung-Hoon; Lee, Jinwon; Yim, Jieun; An, Gynheung

    2003-01-01

    To understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (CvADH1) from the ESTs of developing watermelon seeds. RNA blot analysis and in situ localization showed that CvADH1 was preferentially expressed in the nucellar tissue. The CvADH1 protein shared about 50% homology with short-chain alcohol dehydrogenase including ABA2 in Arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in Forsythia intermedia, and 3beta-hydroxysterol dehydrogenase in Digitalis lanata. We investigated gene-expression levels in seeds from both normally pollinated fruits and those made parthenocarpic via N-(2-chloro-4-pyridyl)-N'-phenylurea treatment, the latter of which lack zygotic tissues. Whereas the transcripts of CvADH1 rapidly started to accumulate from about the pre-heart stage in normal seeds, they were not detectable in the parthenocarpic seeds. Treating the parthenogenic fruit with GA(3) strongly induced gene expression, up to the level accumulated in pollinated seeds. These results suggest that the CvADH1 gene is induced in maternal tissues by signals made in the zygotic tissues, and that gibberellin might be one of those signals. We also observed that CvADH1 expression was induced by sucrose in the parthenocarpic seeds. Therefore, we propose that the CvADH1 gene is inducible by gibberellin, and that sucrose plays an important role in the maternal tissues of watermelon during early seed development. PMID:12552151

  3. Development and chemical and sensory characterization of pumpkin seed flour-based cereal bars

    Directory of Open Access Journals (Sweden)

    Jovane Santana Silva

    2014-06-01

    Full Text Available Pumpkin (Cucurbita maxima, popularly known as squash, is a widely grown vegetable in Brazil. In this study, pumpkin seed flours (PSF with different granulometries were used: PSF 1 (medium granulometry and PSF 2 (coarse granulometry in the preparation of cereal bars (CB with different combinations with brown oats. Five formulations were prepared: CB-1 (control - 25% brown oats and 0% PSF; CB-2 (12.5% PSF 1 and 12.5% brown oats; CB-3 (25% PSF 1 and 0% brown oats; CB-4 (12.5% PSF 2 and 12.5% brown oats; and CB-5 (25% PSF 2 and 0% brown oats. The acceptance test results were analyzed in a conventional preference mapping which indicated that the bars CB-2 and CB-5 received mostly the maximum hedonistic score. With the objective of developing a cereal bar replacing oats with PSF, the bars CB-2 and CB-5 were compared to the conventional bar CB-1. The cereal bars CB-2 and CB-5 showed an increase in crude protein (87.5% and 62.5% and in dietary fiber (77% and 44%, respectively. These results allowed the classification of CB-2 and CB-5 as fiber sources; they can, therefore, be classified as light products according to the Brazilian legislation.

  4. Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae promoted by mycorrhizal fungi Epulorhiza spp.

    Directory of Open Access Journals (Sweden)

    Marlon Corrêa Pereira

    2015-12-01

    Full Text Available Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp. promoted protocorm development until leaf production at 63 days. The protocorms are comprised of parenchyma cells delimited by a unistratified epidermis; the parenchyma cells of the upper part of the protocorms are smaller than those located more towards the base. Intact and digested pelotons were observed inside of protocorms implying that the seedlings were capable of mycotrophy. Additionally, the development of a bud primordium only occurred after colonization by fungus. This study suggests that C. glutiniferum has a preference for strains of Epulorhiza and that fungus digestion is essential to protocorm development.

  5. miRNA regulation in the early development of barley seed

    Directory of Open Access Journals (Sweden)

    Curaba Julien

    2012-07-01

    Full Text Available Abstract Background During the early stages of seed development many genes are under dynamic regulation to ensure the proper differentiation and establishment of the tissue that will constitute the mature grain. To investigate how miRNA regulation contributes to this process in barley, a combination of small RNA and mRNA degradome analyses were used to identify miRNAs and their targets. Results Our analysis identified 84 known miRNAs and 7 new miRNAs together with 96 putative miRNA target genes regulated through a slicing mechanism in grain tissues during the first 15 days post anthesis. We also identified many potential miRNAs including several belonging to known miRNA families. Our data gave us evidence for an increase in miRNA-mediated regulation during the transition between pre-storage and storage phases. Potential miRNA targets were found in various signalling pathways including components of four phytohormone pathways (ABA, GA, auxin, ethylene and the defence response to powdery mildew infection. Among the putative miRNA targets we identified were two essential genes controlling the GA response, a GA3oxidase1 and a homolog of the receptor GID1, and a homolog of the ACC oxidase which catalyses the last step of ethylene biosynthesis. We found that two MLA genes are potentially miRNA regulated, establishing a direct link between miRNAs and the R gene response. Conclusion Our dataset provides a useful source of information on miRNA regulation during the early development of cereal grains and our analysis suggests that miRNAs contribute to the control of development of the cereal grain, notably through the regulation of phytohormone response pathways.

  6. Accumulation of long-lived mRNAs associated with germination in embryos during seed development of rice.

    Science.gov (United States)

    Sano, Naoto; Ono, Hanako; Murata, Kazumasa; Yamada, Tetsuya; Hirasawa, Tadashi; Kanekatsu, Motoki

    2015-07-01

    Mature dry seeds contain translatable mRNAs called long-lived mRNAs. Early studies have shown that protein synthesis during the initial phase of seed germination occurs from long-lived mRNAs, without de novo transcription. However, the gene expression systems that generate long-lived mRNAs in seeds are not well understood. To examine the accumulation of long-lived mRNAs in developing rice embryos, germination tests using the transcriptional inhibitor actinomycin D (Act D) were performed with the Japonica rice cultivar Nipponbare. Although over 70% of embryos at 10 days after flowering (DAF) germinated in the absence of the inhibitor, germination was remarkably impaired in embryos treated with Act D. In contrast, more than 70% of embryos at 20, 25, 30 and 40 DAF germinated in the presence of Act D. The same results were obtained when another cultivar, Koshihikari, was used, indicating that the long-lived mRNAs required for germination predominantly accumulate in embryos between 10 and 20 DAF during seed development. RNA-Seq identified 529 long-lived mRNA candidates, encoding proteins such as ABA, calcium ion and phospholipid signalling-related proteins, and HSP DNA J, increased from 10 to 20 DAF and were highly abundant in 40 DAF embryos of Nipponbare and Koshihikari. We also revealed that these long-lived mRNA candidates are clearly up-regulated in 10 DAF germinating embryos after imbibition, suggesting that the accumulation of these mRNAs in embryos is indispensable for the induction of germination. The findings presented here may facilitate in overcoming irregular seed germination or producing more vigorous seedlings. PMID:25941326

  7. Development and validation of the Screen for Early Eating Disorder Signs (SEEDS) in persons with type 1 diabetes.

    Science.gov (United States)

    Powers, Margaret A; Richter, Sara; Ackard, Diann; Craft, Cheryl

    2016-01-01

    This study's objective was to develop and validate an instrument to identify those at risk of developing an eating disorder (ED) in persons with type 1 diabetes. The Screen for Early Eating Disorder Signs (SEEDS) instrument was developed using a multi-phase process including focus groups, cognitive interviews, and mailed questionnaires. Factor analysis revealed 20 items across three factors (Body Image, Feelings, Quality of Life) demonstrating strong psychometric properties. Scoring guidelines and interpretation are provided. SEEDS is a brief (20-item; 2-5 minutes to complete), self-administered, screen designed for use in clinical practice or research to identify or confirm suspicions of ED risk and does not include weight-control behavior items. PMID:26467220

  8. Developing an Enterprise Architecture Foundation – Case Study of a Global Manufacturing Company

    OpenAIRE

    Kralev, Trajche

    2015-01-01

    During the past two decades, organizations have rushed into information technology for the potential it offers. Over time, the complexity of these implementations increases and begins causing problems for the business. To deal with these problems organizations are looking at enterprise architecture (EA). This case study, at a global manufacturing company, explores how organizations can begin implementing enterprise architecture. We study the meaning, challenges, benefits and the process o...

  9. DENDRITIC ARCHITECTURE: A NEW TOOL FOR DEVELOPMENT OF NOVEL DRUG DELIVERY SYSTEMS

    OpenAIRE

    Ram Kumar Choudhary; P. Vijayaraj Kumar; K.N. Jayaveera

    2012-01-01

    Dendrimers are composed of a multifunctional core molecule attached to polymer branches. Drugs can be inserted between the branches and held until ‘interactions’ release it. Dendrimers are a new class of three-dimensional, man-made molecules produced by an unusual synthetic route which incorporates repetitive branching sequences to create a unique novel architecture. Exceptional features of the dendritic architecture include a high degree of structural symmetry, a density gradient displaying ...

  10. Implementation and development of data center network architecture for the eu lightness project

    OpenAIRE

    Panahi, Nogol

    2014-01-01

    Internet-based business and consumer applications are driving serious increment in traffic to datacenters. Current multi-tier and over provision data center network architectures with significant cost, complexity, and power consumption are unable to provide adequate throughput and performance according to the increasing traffic growth and Information Technology (IT) demands. The features of recently proposed data center network architectures such as dynamic routing, flexible bandwidth, SDN in...

  11. Stage-Specific Fatty Acid Fluxes Play a Regulatory Role in Glycerolipid Metabolism during Seed Development in Jatropha curcas L.

    Science.gov (United States)

    Chaitanya, Bharatula Sri Krishna; Kumar, Sumit; Kaki, Shiva Shanker; Balakrishna, Marrapu; Karuna, Mallampalli Sri Lakshmi; Prasad, Rachapudi Badari Narayana; Sastry, Pidaparty Seshadri; Reddy, Attipalli Ramachandra

    2015-12-23

    The present study describes the changes in lipid profile as well as fatty acid fluxes during seed development in Jatropha curcas L. Endosperm from 34, 37, and 40 days after anthesis (DAA), incubated with [(14)C]acetate, showed significant synthesis of phosphatidylcholine (PC) at seed maturation. The fatty acid methyl ester profile showed PC from 34 DAA was rich in palmitic acid (16:0), whereas PC from 37 and 40 DAA was rich in oleic acid (18:1n-9). Molecular species analysis of diacylglycerol (DAG) indicated DAG (16:0/18:2n-6) was in abundance at 34 DAA, whereas DAG (18:1n-9/18:2n-6) was significantly high at 40 DAA. Triacylglycerol (TAG) analysis revealed TAG (16:0/18:2n-6/16:0) was abundant at 34 DAA, whereas TAG (18:1n-9/18:2n-6/18:1n-9) formed the majority at 40 DAA. Expression of two types of diacylglycerol acyltransferases varied with seed maturation. These data demonstrate stage-specific distinct pools of PC and DAG synthesis during storage TAG accumulation in Jatropha seed. PMID:26628196

  12. Development, Physico-Chemical and Sensory Properties of Biscuits Supplemented with Pumpkin Seeds to Combat Childhood Malnutrition in Pakistan

    International Nuclear Information System (INIS)

    This study was carried out in Food Science and Product Development Institute, National Agricultural Research Centre, Islamabad, Pakistan in 2014. Nutritive pumpkin seed flour fortified biscuits were prepared with four different substituted levels of pumpkin seed flour in wheat flour (T/sub 2/ = 5%, T/sub 3/ = 10%, T/sub 4/ = 15% and T/sub 5/ = 20%), were compared with control (T/sub 1/). Chemical attributes of biscuits showed that T/sub 5/ has maximum level of pumpkin flour (20%) with maximum protein (12.30%), fat (28.29%), ash (4.13%), iron (2.28%) and zinc (3.11%). Sensory results also revealed increasing trend in all sensory parameters. Results showed acceptability at all levels but treatment T/sub 4/ with 15 % pumpkin seed flour scored highest (8.0) for maximum overall acceptability. It was concluded that pumpkin seed flour can be supplemented successfully to partially replace wheat flour to prepare highly nutritious biscuits without affecting its overall acceptability. (author)

  13. Development and appraisal of economical and sustainable approach for weed management in drill seeded aerobic rice (oryza sativa l.)

    International Nuclear Information System (INIS)

    Conventional rice cultivation by puddling and transplanting is a labor intensive activity. Water scarcity is a threat for the sustain ability of transplanted rice. In many areas of Asia, rice transplantation of rice is being replaced by direct seeding as farmers tried to solve the problems of labor cost and water scarcity but weed control is one of the major constraints to direct seeding. So, to control weeds in direct seeded rice present studies were designed. A two years study was conducted to develop sustainable and economical methods for managing weeds in aerobic rice grown by dry direct-seeding at Student's Farm, Department of Agronomy, University of Agriculture, Faisalabad during the years 2008 and 2009. Experiment was laid out in RCBD with five weed management strategies: hand weeding, hoeing with kasula, inter-row cultivation with tine cultivator, inter-row cultivation with spike hoe and chemical control with Nominee 100 SC along with control (no weeding). Weed dry weight was 300 g m/sup -2/, 257 g m/sup -2/, 225 g m/sup -2/ and 157 g m/sup -2/ less in hand weeding, hoeing, tine cultivator and Nominee 100 SC respectively than no weeding. Paddy yield was 221%, 203%, 181% and 105% more in hand weeding, hoeing, tine cultivator and Nominee 100 SC respectively than no weeding. (author)

  14. Effect of cultural conditions on the seed-to-seed growth of Arabidopsis and Cardamine - A study of growth rates and reproductive development as affected by test tube seals

    Science.gov (United States)

    Hoshizaki, T.

    1982-01-01

    The effects of test tube seals on the growth, flowering, and seed pod formation of Arabidopsis thaliana (L.) Heynh., mouse ear cress, and Cardamine oligosperma Nutt, bitter cress, are studied in order to assess the conditions used in weightlessness experiments. Among other results, it is found that the growth (height) and flowering (date of bud appearance) were suppressed in mouse ear cress in tubes sealed with Saran. Seed pod formation which occurred by day 45 in open-to-air controls, was still lacking in the sealed plants even up to day 124. The growth and flowering of bitter cress were also suppressed by the Saran seal, although up to day 55 the Saran-sealed plants were taller. It is suggested that atmospheric composition was the cause of the suppression of growth, flowering, and seed pod development in these plants, since the mouse ear cress renewed their growth and then set seed pods after the Saran seal was ruptured.

  15. Robust E-Health Communication Architecture for Rural Communities in Developing Countries

    Directory of Open Access Journals (Sweden)

    F. E. Idachaba

    2012-06-01

    Full Text Available The lack of access to quality healthcare and the lack of sufficient manpower, especially doctors, in rural areas is a major healthcare challenge faced by dwellers in rural communities in most developing countries. The intervention of governments, which range from the introduction of training programs aimed at producing health workers from schools of health technology and nursing to the recalling and deployment of retired nurses to rural areas, has still not been able to generate the desired improvement in healthcare delivery, as outbreaks of epidemics are still being reported in such areas. This work presents an E-Health architecture which utilizes low cost sensors and communication devices to link the doctors in the urban areas with the patients in rural areas enabling doctors-patients interaction. The system enables accurate and timely diagnosis of the patients and facilitates proper treatment plans. It also incorporates an epidemic alert which enables the tracking of diseases and the early detection and control of epidemics.

  16. Loss of Usp9x disrupts cortical architecture, hippocampal development and TGFβ-mediated axonogenesis.

    Directory of Open Access Journals (Sweden)

    Shane Stegeman

    Full Text Available The deubiquitylating enzyme Usp9x is highly expressed in the developing mouse brain, and increased Usp9x expression enhances the self-renewal of neural progenitors in vitro. USP9X is a candidate gene for human neurodevelopmental disorders, including lissencephaly, epilepsy and X-linked intellectual disability. To determine if Usp9x is critical to mammalian brain development we conditionally deleted the gene from neural progenitors, and their subsequent progeny. Mating Usp9x(loxP/loxP mice with mice expressing Cre recombinase from the Nestin promoter deleted Usp9x throughout the entire brain, and resulted in early postnatal lethality. Although the overall brain architecture was intact, loss of Usp9x disrupted the cellular organization of the ventricular and sub-ventricular zones, and cortical plate. Usp9x absence also led to dramatic reductions in axonal length, in vivo and in vitro, which could in part be explained by a failure in Tgf-β signaling. Deletion of Usp9x from the dorsal telencephalon only, by mating with Emx1-cre mice, was compatible with survival to adulthood but resulted in reduction or loss of the corpus callosum, a dramatic decrease in hippocampal size, and disorganization of the hippocampal CA3 region. This latter phenotypic aspect resembled that observed in Doublecortin knock-out mice, which is an Usp9x interacting protein. This study establishes that Usp9x is critical for several aspects of CNS development, and suggests that its regulation of Tgf-β signaling extends to neurons.

  17. Proteomic analysis of oil body membrane proteins accompanying the onset of desiccation phase during sunflower seed development.

    Science.gov (United States)

    Thakur, Anita; Bhatla, Satish C

    2015-01-01

    A noteworthy metabolic signature accompanying oil body (OB) biogenesis during oilseed development is associated with the modulation of the oil body membranes proteins. Present work focuses on 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE)-based analysis of the temporal changes in the OB membrane proteins analyzed by LC-MS/MS accompanying the onset of desiccation (20-30 d after anthesis; DAA) in the developing seeds of sunflower (Helianthus annuus L.). Protein spots unique to 20-30 DAA stages were picked up from 2-D gels for identification and the identified proteins were categorized into 7 functional classes. These include proteins involved in energy metabolism, reactive oxygen scavenging, proteolysis and protein turnover, signaling, oleosin and oil body biogenesis-associated proteins, desiccation and cytoskeleton. At 30 DAA stage, exclusive expressions of enzymes belonging to energy metabolism, desiccation and cytoskeleton were evident which indicated an increase in the metabolic and enzymatic activity in the cells at this stage of seed development (seed filling). Increased expression of cruciferina-like protein and dehydrin at 30 DAA stage marks the onset of desiccation. The data has been analyzed and discussed to highlight desiccation stage-associated metabolic events during oilseed development. PMID:26786011

  18. Evolution of contemporary museum architecture

    OpenAIRE

    Bilous, Yulia

    2013-01-01

    This article deals with the development of museum architecture from the formation of the classic building architecture to the establishment of the contemporary museum architecture. Changes in the museum building architecture and displaying principles have been analysed. The 19th century was defined by the emergence of a vast number of museums serving through present as examples of the contemporary museum architecture. New styles are tried in the museum architecture alo...

  19. Hybrid architecture: object, landscape, infrastructure

    OpenAIRE

    Pinto de Freitas, Rita

    2011-01-01

    The concept of "hybrid architecture" developed in this study considers hybrid all architecture that is at once object, landscape and infrastructure. Hybrid architecture, pushed by the fact that it concentrates in a single architectural intervention a triple object-, landscape- and infrastructure-related nature, generates architectural answers with very specific features, and its study achieves following goals: 1: Clarify the term hybrid related to architectural intervention; 2: Tran...

  20. Confocal analysis of nervous system architecture in direct-developing juveniles of Neanthes arenaceodentata (Annelida, Nereididae

    Directory of Open Access Journals (Sweden)

    Jacobs David K

    2010-06-01

    Full Text Available Abstract Background Members of Family Nereididae have complex neural morphology exemplary of errant polychaetes and are leading research models in the investigation of annelid nervous systems. However, few studies focus on the development of their nervous system morphology. Such data are particularly relevant today, as nereidids are the subjects of a growing body of "evo-devo" work concerning bilaterian nervous systems, and detailed knowledge of their developing neuroanatomy facilitates the interpretation of gene expression analyses. In addition, new data are needed to resolve discrepancies between classic studies of nereidid neuroanatomy. We present a neuroanatomical overview based on acetylated α-tubulin labeling and confocal microscopy for post-embryonic stages of Neanthes arenaceodentata, a direct-developing nereidid. Results At hatching (2-3 chaetigers, the nervous system has developed much of the complexity of the adult (large brain, circumesophageal connectives, nerve cords, segmental nerves, and the stomatogastric nervous system is partially formed. By the 5-chaetiger stage, the cephalic appendages and anal cirri are well innervated and have clear connections to the central nervous system. Within one week of hatching (9-chaetigers, cephalic sensory structures (e.g., nuchal organs, Langdon's organs and brain substructures (e.g., corpora pedunculata, stomatogastric ganglia are clearly differentiated. Additionally, the segmental-nerve architecture (including interconnections matches descriptions of other, adult nereidids, and the pharynx has developed longitudinal nerves, nerve rings, and ganglia. All central roots of the stomatogastric nervous system are distinguishable in 12-chaetiger juveniles. Evidence was also found for two previously undescribed peripheral nerve interconnections and aspects of parapodial muscle innervation. Conclusions N. arenaceodentata has apparently lost all essential trochophore characteristics typical of

  1. Federal Data Repository Research: Recent Developments in Mercury Search System Architecture

    Science.gov (United States)

    Devarakonda, R.

    2015-12-01

    New data intensive project initiatives needs new generation data system architecture. This presentation will discuss the recent developments in Mercury System [1] including adoption, challenges, and future efforts to handle such data intensive projects. Mercury is a combination of three main tools (i) Data/Metadata registration Tool (Online Metadata Editor): The new Online Metadata Editor (OME) is a web-based tool to help document the scientific data in a well-structured, popular scientific metadata formats. (ii) Search and Visualization Tool: Provides a single portal to information contained in disparate data management systems. It facilitates distributed metadata management, data discovery, and various visuzalization capabilities. (iii) Data Citation Tool: In collaboration with Department of Energy's Oak Ridge National Laboratory (ORNL) Mercury Consortium (funded by NASA, USGS and DOE), established a Digital Object Identifier (DOI) service. Mercury is a open source system, developed and managed at Oak Ridge National Laboratory and is currently being funded by three federal agencies, including NASA, USGS and DOE. It provides access to millions of bio-geo-chemical and ecological data; 30,000 scientists use it each month. Some recent data intensive projects that are using Mercury tool: USGS Science Data Catalog (http://data.usgs.gov/), Next-Generation Ecosystem Experiments (http://ngee-arctic.ornl.gov/), Carbon Dioxide Information Analysis Center (http://cdiac.ornl.gov/), Oak Ridge National Laboratory - Distributed Active Archive Center (http://daac.ornl.gov), SoilSCAPE (http://mercury.ornl.gov/soilscape). References: [1] Devarakonda, Ranjeet, et al. "Mercury: reusable metadata management, data discovery and access system." Earth Science Informatics 3.1-2 (2010): 87-94.

  2. Development of a small single-ring OpenPET prototype with a novel transformable architecture

    Science.gov (United States)

    Tashima, Hideaki; Yoshida, Eiji; Inadama, Naoko; Nishikido, Fumihiko; Nakajima, Yasunori; Wakizaka, Hidekatsu; Shinaji, Tetsuya; Nitta, Munetaka; Kinouchi, Shoko; Suga, Mikio; Haneishi, Hideaki; Inaniwa, Taku; Yamaya, Taiga

    2016-02-01

    The single-ring OpenPET (SROP), for which the detector arrangement has a cylinder shape cut by two parallel planes at a slant angle to form an open space, is our original proposal for in-beam PET. In this study, we developed a small prototype of an axial-shift type SROP (AS-SROP) with a novel transformable architecture for a proof-of-concept. In the AS-SROP, detectors originally forming a cylindrical PET are axially shifted little by little. We designed the small AS-SROP prototype for 4-layer depth-of-interaction detectors arranged in a ring diameter of 250 mm. The prototype had two modes: open and closed. The open mode formed the SROP with the open space of 139 mm and the closed mode formed a conventional cylindrical PET. The detectors were simultaneously moved by a rotation handle allowing them to be transformed between the two modes. We evaluated the basic performance of the developed prototype and carried out in-beam imaging tests in the HIMAC using 11C radioactive beam irradiation. As a result, we found the open mode enabled in-beam PET imaging at a slight cost of imaging performance; the spatial resolution and sensitivity were 2.6 mm and 5.1% for the open mode and 2.1 mm and 7.3% for the closed mode. We concluded that the AS-SROP can minimize the decrease of resolution and sensitivity, for example, by transforming into the closed mode immediately after the irradiation while maintaining the open space only for the in-beam PET measurement.

  3. Proteome analysis of pod and seed development in the model legume Lotus japonicus

    DEFF Research Database (Denmark)

    Nautrup-Pedersen, G.; Dam, S.; Laursen, B. S.;

    2010-01-01

    . Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis...... of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins...... and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions...

  4. Potassium leakage and maize seed physiological potential

    OpenAIRE

    Miguel Mariane Victorio de Carvalho; Marcos Filho Julio

    2002-01-01

    Seed production usually requires fast decisions to improve the efficacy of seed handling during harvesting, processing and storage. Seed technologists have focused on the development or improvement of procedures which allow rapid and consistent identification of higher quality seed lots. This research verified the effectiveness of the potassium leachate test on the evaluation of the physiological potential of maize seeds in comparison to recommended seed vigor tests. Five seed lots of the hyb...

  5. The effects of free amino acids profiles on seeds germination/dormancy and seedlings development of two genetically different cultivars of Yemeni Pomegranates

    Directory of Open Access Journals (Sweden)

    Alhadi Fatima A.

    2012-04-01

    seeds germination in plant, therefore Khazemi germination capacity was assumed to be regulated more or less by these AAs. In addition, changes in amino acid composition in the germinated Khazemi cultivar during various stages of seeds germination including imbibition, germination, and sprouts stages have been noticed to change in response with germination demands. This suggests that amino acids reserves in dry seeds are major determinant for germination capacity and germination behavior in the following steps of germination. The noticed particular AAs increase/decrease along the time course of Khazemi pomegranate germination till establishment of heterotrophic seedlings were used as cornerstones for elucidation and deduction of putative function and relevant biochemical pathways controlling initiation of seeds germination and seedlings developments. Based on publicly available databases of model plants and literatures surveys, we established correlations between prevailing AAs factors as biochemical parameters actively involved in seeds dormancy-breaking and germination process.

  6. Study of Dosimetric and Thermal Properties of a Newly Developed Thermo-brachytherapy Seed for Treatment of Solid Tumors

    Science.gov (United States)

    Gautam, Bhoj R.

    Studies on the curative effects of hyperthermia and radiation therapy on treatment of cancer show strong evidence of synergistic enhancement when both radiation and hyperthermia treatment modalities are applied simultaneously. A variety of tissue heating approaches developed to date still fail to overcome essential limitations such as inadequate temperature control, temperature non-uniformity, and prolonged time delay between hyperthermia and radiation treatments. We propose a new self-regulating Thermo-brachytherapy (TB) seed, which serves as a source of both radiation and heat for concurrent administration of brachytherapy and hyperthermia. The proposed seed is based on the BestRTM Iodine-125 seed model 2301, where the tungsten marker core and the air gap are replaced with ferromagnetic material. The ferromagnetic core produces heat when subjected to an alternating electromagnetic (EM) field and effectively shuts off after reaching the Curie temperature (TC) of the ferromagnetic material, thus establishing temperature self-regulation. The seed has a ferromagnetic Ni-Cu alloy core having a Curie transition at a temperature of 52 °C. This study summarizes the design and development of the self regulating ferromagnetic core TB seed for the concurrent hyperthermia and brachytherapy treatments. An experimental study of the magnetic properties of the Ni1-xCu x (0.28≤ x ≤0.3) alloys, and the simulation studies of radiation and thermal distribution properties of the seed have been performed. A preliminary experiment for the ferromagnetic induction heating of Ni-Cu needles has been carried out to ensure the practical feasibility of the induction heating. Radiation dose characterizing parameters (dose rate constant and other TG-43 factors) were calculated using the Monte Carlo method. For the thermal characteristics, we studied a model consisting of single or multiple seeds placed in the central region of a cylindrical phantom using a finite-element analysis method

  7. Applied Nuclear Accountability Systems: A Case Study in the System Architecture and Development of NuMAC

    International Nuclear Information System (INIS)

    This is a case study of the NuMAC nuclear accountability system developed at a private fuel fabrication facility. This paper investigates nuclear material accountability and safeguards by researching expert knowledge applied in the system design and development. Presented is a system developed to detect and deter the theft of weapons grade nuclear material. Examined is the system architecture that includes: issues for the design and development of the system; stakeholder issues; how the system was built and evolved; software design, database design, and development tool considerations; security and computing ethics. (author)

  8. The Swedish strategy and method for development of a national healthcare information architecture.

    Science.gov (United States)

    Rosenälv, Jessica; Lundell, Karl-Henrik

    2012-01-01

    "We need a precise framework of regulations in order to maintain appropriate and structured health care documentation that ensures that the information maintains a sufficient level of quality to be used in treatment, in research and by the actual patient. The users shall be aided by clearly and uniformly defined terms and concepts, and there should be an information structure that clarifies what to document and how to make the information more useful. Most of all, we need to standardize the information, not just the technical systems." (eHälsa - nytta och näring, Riksdag report 2011/12:RFR5, p. 37). In 2010, the Swedish Government adopted the National e-Health - the national strategy for accessible and secure information in healthcare. The strategy is a revision and extension of the previous strategy from 2006, which was used as input for the most recent efforts to develop a national information structure utilizing business-oriented generic models. A national decision on healthcare informatics standards was made by the Swedish County Councils, which decided to follow and use EN/ISO 13606 as a standard for the development of a universally applicable information structure, including archetypes and templates. The overall aim of the Swedish strategy for development of National Healthcare Information Architecture is to achieve high level semantic interoperability for clinical content and clinical contexts. High level semantic interoperability requires consistently structured clinical data and other types of data with coherent traceability to be mapped to reference clinical models. Archetypes that are formal definitions of the clinical and demographic concepts and some administrative data were developed. Each archetype describes the information structure and content of overarching core clinical concepts. Information that is defined in archetypes should be used for different purposes. Generic clinical process model was made concrete and analyzed. For each decision

  9. A survey of sustainability and passive design curriculum development in schools of architecture

    Energy Technology Data Exchange (ETDEWEB)

    Boake, T.M. [Univ. of Waterloo, Ontario (Canada). School of Architecture

    1996-10-01

    Although there seems to be much abuzz regarding the teaching of Sustainability to students of Architecture, the current state of this teaching is not clearly defined. In an effort to understand the present condition of Sustainable education the author has conducted a United States-Canada Survey on the influence of Sustainability and Passive Design on curricular content and direction in Schools of Architecture. The purpose of this paper is to present a snapshot of the current status of Passive and Sustainable teaching; address issues in approach related to regionality--on both a geographic and climatic basis; and review preferred teaching resource packages, texts, videos and software. The analysis which follows was based on a Survey which was sent to faculty of Schools of Architecture in Canada and the United States over a 121 month period from January to December 1995. This paper reflects the January 96 status of the analysis.

  10. A Scalable Wireless Sensor Network (WSN Based Architecture for Fire Disaster Monitoring in the Developing World

    Directory of Open Access Journals (Sweden)

    Emmanuel Lule

    2015-01-01

    Full Text Available All over the world, there is a tremendous increase in disaster occurrences such as landslide, toxic gas pollutions and wild fires. Least developed countries like Uganda have taken minimal efforts in management and containment of such disasters on behalf of the local populace. The dangers of fires are as a result of lack of proper information about the location, intensity and rate of spread of fire. However, the use of WSN may be one of the alternative ways of reducing risks associated with spreading fires resulting into destruction of lives and property worth millions of shillings. Our research looks at fire monitoring using sensors deployed in an event field to detect possible precursors of a fire occurrence using a simulated approach of OPNET Modeler (Ver. 14.0. Mobile nodes were deployed in proximity of (500X500 m2 flat space moving at a uniform speed of 10ms-1 for 30 minutes. Our simulations are based on ftp high priority traffic to reflect real time information transfer for analysis. This paper evaluates and discusses how sensed information can be transmitted through a network with minimal delay and proposes a scalable WSN architectural design based on protocol perspective i.e. AODV and DSR. Two QoS parameters have been considered i.e. delay and throughput. Our results show that AODV scheme has got a minimum delay of 0.2ms-1and a maximum throughput of 1.7Mbps. Hence it's highly recommended for monitoring fires in large open area settings compared to DSR that is suitable for smaller areas. Results show that DSR exhibits higher delays due to nodal congestion and high control overheads. However, introducing the proposed heterogeneous routing mechanism i.e. (AODV+DSR into the network significantly improves the performance of DSR.

  11. Automation Hooks Architecture for Flexible Test Orchestration - Concept Development and Validation

    Science.gov (United States)

    Lansdowne, C. A.; Maclean, John R.; Winton, Chris; McCartney, Pat

    2011-01-01

    The Automation Hooks Architecture Trade Study for Flexible Test Orchestration sought a standardized data-driven alternative to conventional automated test programming interfaces. The study recommended composing the interface using multicast DNS (mDNS/SD) service discovery, Representational State Transfer (Restful) Web Services, and Automatic Test Markup Language (ATML). We describe additional efforts to rapidly mature the Automation Hooks Architecture candidate interface definition by validating it in a broad spectrum of applications. These activities have allowed us to further refine our concepts and provide observations directed toward objectives of economy, scalability, versatility, performance, severability, maintainability, scriptability and others.

  12. Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development

    DEFF Research Database (Denmark)

    Nogueira, Fábio C S; Palmisano, Giuseppe; Soares, Emanoella L; Shah, Mohibullah; Soares, Arlete A; Roepstorff, Peter; Campos, Francisco A P; Domont, Gilberto B

    2012-01-01

    In this study, we performed a proteomic analysis of nucellus from two developmental stages of Ricinus communis seeds by a GeLC-MS/MS approach, using of a high resolution orbitrap mass spectrometer, which resulted in the identification of a total of 766 proteins that were grouped into 553 protein ...

  13. Calendula oil processing : seed classification, oil extraction, refining process development and oil quality aspects

    NARCIS (Netherlands)

    Janssens, R.J.J.

    2000-01-01

    The difference in Calendula oil quality from fractions obtained after seed classification is enormous. The oil quality varies from excellent to very poor, according to important aspects such as in the hulls and dust fraction, high free fatty acid values (13% vs. 0.6%) are found. This can be explaine

  14. Seeds of confusion : the impact of policies on seed systems

    OpenAIRE

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important development issue. This study deals with the impact different types of regulation have on how farmers access seed.  I have analysed current regulatory frameworks in terms of their impact on differ...

  15. Studies on the development of 169Yb-brachytherapy seeds: New generation brachytherapy sources for the management of cancer

    International Nuclear Information System (INIS)

    This paper describes development of 169Yb-seeds by encapsulating 0.6–0.65 mm (ϕ) sized 169Yb2O3 microspheres in titanium capsules. Microspheres synthesized by a sol–gel route were characterized by XRD, SEM/EDS and ICP-AES. Optimization of neutron irradiation was accomplished and 169Yb-seeds up to 74 MBq of 169Yb could be produced from natural Yb2O3 microspheres, which have the potential for use in prostate brachytherapy. A protocol to prepare 169Yb-brachytherapy sources (2.96–3.7 TBq of 169Yb) with the use of enriched targets was also formulated

  16. Three-Dimensional Imaging of the Developing Vasculature within Stem Cell-Seeded Scaffolds Cultured in ovo

    Science.gov (United States)

    Woloszyk, Anna; Liccardo, Davide; Mitsiadis, Thimios A.

    2016-01-01

    Successful tissue engineering requires functional vascularization of the three-dimensional constructs with the aim to serve as implants for tissue replacement and regeneration. The survival of the implant is only possible if the supply of oxygen and nutrients by developing capillaries from the host is established. The chorioallantoic membrane (CAM) assay is a valuable tool to study the ingrowth and distribution of vessels into scaffolds composed by appropriate biomaterials and stem cell populations that are used in cell-based regenerative approaches. The developing vasculature of chicken embryos within cell-seeded scaffolds can be visualized with microcomputed tomography after intravenous injection of MicroFil®, which is a radiopaque contrast agent. Here, we provide a step-by-step protocol for the seeding of stem cells into silk fibroin scaffolds, the CAM culture conditions, the procedure of MicroFil® perfusion, and finally the microcomputed tomography scanning. Three-dimensional imaging of the vascularized tissue engineered constructs provides an important analytical tool for studying the potential of cell seeded scaffolds to attract vessels and form vascular networks, as well as for analyzing the number, density, length, branching, and diameter of vessels. This in ovo method can greatly help to screen implants that will be used for tissue regeneration purposes before their in vivo testing, thereby reducing the amount of animals needed for pre-clinical studies. PMID:27148081

  17. Development and Industrialization of InGaN/GaN LEDs on Patterned Sapphire Substrates for Low Cost Emitter Architecture

    Energy Technology Data Exchange (ETDEWEB)

    Flemish, Joseph [Lumileds LLC., San Jose, CA (United States); Soer, Wouter [Lumileds LLC., San Jose, CA (United States)

    2015-11-30

    Patterned sapphire substrate (PSS) technology has proven to be an effective approach to improve efficacy and reduce cost of light-emitting diodes (LEDs). The volume emission from the transparent substrate leads to high package efficiency, while the simple and robust architecture of PSS-based LEDs enables low cost. PSS substrates have gained wide use in mid-power LEDs over the past years. In this project, Lumileds has developed and industrialized PSS and epitaxy technology for high- power flip-chip LEDs to bring these benefits to a broader range of applications and accelerate the adoption of energy-efficient solid-state lighting (SSL). PSS geometries were designed for highly efficient light extraction in a flip-chip architecture and high-volume manufacturability, and corresponding sapphire patterning and epitaxy manufacturing processes were integrally developed. Concurrently, device and package architectures were developed to take advantage of the PSS flip-chip die in different types of products that meet application needs. The developed PSS and epitaxy technology has been fully implemented in manufacturing at Lumileds’ San Jose, CA location, and incorporated in illumination-grade LED products that have been successfully introduced to the market, including LUXEON Q and LUXEON FlipChip White.

  18. Heterosis in plants: Manifestation in early seed development and prediction approaches to assist hybrid breeding

    Institute of Scientific and Technical Information of China (English)

    THIEMANN Alexander; MEYER Stephanie; SCHOLTEN Stefan

    2009-01-01

    Heterosis, or hybrid vigour, describes the superior performance of heterozygous hybrid individuals compared with their homozygous parental inbred lines. Although heterosis has been intensively used in plant breeding, the molecular and genetic mechanism underlying the phenomenon remains largely unknown. During the last years numerous laboratories initiated genomic approaches with new, often genome wide tools toward the elucidation of the molecular basis of heterosis. Various studies described differences in genome organization and gene expression of hybrids and their parental inbred lines. In maize, a considerable loss of co-linearity at many loci between different inbred lines was observed. Expression profile comparisons between inbred lines and hybrids revealed complex transcrip-tional networks specific for different developmental stages and tissues mainly in maize (Zea mays), rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana). Allele-specific expression data resolved the relative parental contributions and allowed figuring out the regulatory basis for expression variation in hybrids. Integrating all these complex expression data might help to get an idea about the molecular basis of heterosis. Thereby molecular processes during early seed development shortly after fertilization might be of particular importance, because the allelic interplay has to be coordinated after the unification of two diverse genomes and these processes might contribute to establish the basis for future performance of the sporophyte. Besides these fundamental interests in the molecular basis and manifestation of hybrid vigour applied aspects of the phenomenon are of high importance to support plant breeding and agriculture. Prediction methods are of special interest to identify the most promising parental lines of hybrid varieties, greatly reducing the financial effort and increasing the efficiency to develop new hybrid cultivars. Until now, most prediction approaches were based on

  19. Space station data system analysis/architecture study. Task 2: Options development, DR-5. Volume 3: Programmatic options

    Science.gov (United States)

    1985-01-01

    Task 2 in the Space Station Data System (SSDS) Analysis/Architecture Study is the development of an information base that will support the conduct of trade studies and provide sufficient data to make design/programmatic decisions. This volume identifies the preferred options in the programmatic category and characterizes these options with respect to performance attributes, constraints, costs, and risks. The programmatic category includes methods used to administrate/manage the development, operation and maintenance of the SSDS. The specific areas discussed include standardization/commonality; systems management; and systems development, including hardware procurement, software development and system integration, test and verification.

  20. Integration of a constraint-based metabolic model of Brassica napus developing seeds with 13C-Metabolic Flux Analysis

    Directory of Open Access Journals (Sweden)

    Jordan eHay

    2014-12-01

    Full Text Available The use of large-scale or genome-scale metabolic reconstructions for modeling and simulation of plant metabolism and integration of those models with large-scale omics and experimental flux data is becoming increasingly important in plant metabolic research. Here we report an updated version of bna572, a bottom-up reconstruction of oilseed rape (Brassica napus L.; Brassicaceae developing seeds with emphasis on representation of biomass-component biosynthesis. New features include additional seed-relevant pathways for isoprenoid, sterol, phenylpropanoid, flavonoid, and choline biosynthesis. Being now based on standardized data formats and procedures for model reconstruction, bna572+ is available as a COBRA-compliant Systems Biology Markup Language (SBML model and conforms to the Minimum Information Requested in the Annotation of Biochemical Models (MIRIAM standards for annotation of external data resources. Bna572+ contains 966 genes, 671 reactions, and 666 metabolites distributed among 11 subcellular compartments. It is referenced to the Arabidopsis thaliana genome, with gene-protein-reaction associations resolving subcellular localization. Detailed mass and charge balancing and confidence scoring were applied to all reactions. Using Brassica napus seed specific transcriptome data, expression was verified for 78% of bna572+ genes and 97% of reactions. Alongside bna572+ we also present a revised carbon centric model for 13C-Metabolic Flux Analysis (13C-MFA with all its reactions being referenced to bna572+ based on linear projections. By integration of flux ratio constraints obtained from 13C-MFA and by elimination of infinite flux bounds around thermodynamically infeasible loops based on COBRA loopless methods, we demonstrate improvements in predictive power of Flux Variability Analysis (FVA. Using this combined approach we characterize the difference in metabolic flux of developing seeds of two Brassica napus genotypes contrasting in starch and

  1. Architectural Slicing

    DEFF Research Database (Denmark)

    Christensen, Henrik Bærbak; Hansen, Klaus Marius

    2013-01-01

    Architectural prototyping is a widely used practice, con- cerned with taking architectural decisions through experiments with light- weight implementations. However, many architectural decisions are only taken when systems are already (partially) implemented. This is prob- lematic in the context of...... architectural prototyping since experiments with full systems are complex and expensive and thus architectural learn- ing is hindered. In this paper, we propose a novel technique for harvest- ing architectural prototypes from existing systems, \\architectural slic- ing", based on dynamic program slicing. Given a...... system and a slicing criterion, architectural slicing produces an architectural prototype that contain the elements in the architecture that are dependent on the ele- ments in the slicing criterion. Furthermore, we present an initial design and implementation of an architectural slicer for Java....

  2. Protein disulfide isomerase-like protein 1-1 controls endosperm development through regulation of the amount and composition of seed proteins in rice.

    Directory of Open Access Journals (Sweden)

    Yeon Jeong Kim

    Full Text Available Protein disulfide isomerase (PDI is a chaperone protein involved in oxidative protein folding by acting as a catalyst and assisting folding in the endoplasmic reticulum (ER. A genome database search showed that rice contains 19 PDI-like genes. However, their functions are not clearly identified. This paper shows possible functions of rice PDI-like protein 1-1 (PDIL1-1 during seed development. Seeds of the T-DNA insertion PDIL1-1 mutant, PDIL1-1Δ, identified by genomic DNA PCR and western blot analysis, display a chalky phenotype and a thick aleurone layer. Protein content per seed was significantly lower and free sugar content higher in PDIL1-1Δ mutant seeds than in the wild type. Proteomic analysis of PDIL1-1Δ mutant seeds showed that PDIL1-1 is post-translationally regulated, and its loss causes accumulation of many types of seed proteins including glucose/starch metabolism- and ROS (reactive oxygen species scavenging-related proteins. In addition, PDIL1-1 strongly interacts with the cysteine protease OsCP1. Our data indicate that the opaque phenotype of PDIL1-1Δ mutant seeds results from production of irregular starch granules and protein body through loss of regulatory activity for various proteins involved in the synthesis of seed components.

  3. A Systems Approach to Developing an Affordable Space Ground Transportation Architecture using a Commonality Approach

    Science.gov (United States)

    Garcia, Jerry L.; McCleskey, Carey M.; Bollo, Timothy R.; Rhodes, Russel E.; Robinson, John W.

    2012-01-01

    This paper presents a structured approach for achieving a compatible Ground System (GS) and Flight System (FS) architecture that is affordable, productive and sustainable. This paper is an extension of the paper titled "Approach to an Affordable and Productive Space Transportation System" by McCleskey et al. This paper integrates systems engineering concepts and operationally efficient propulsion system concepts into a structured framework for achieving GS and FS compatibility in the mid-term and long-term time frames. It also presents a functional and quantitative relationship for assessing system compatibility called the Architecture Complexity Index (ACI). This paper: (1) focuses on systems engineering fundamentals as it applies to improving GS and FS compatibility; (2) establishes mid-term and long-term spaceport goals; (3) presents an overview of transitioning a spaceport to an airport model; (4) establishes a framework for defining a ground system architecture; (5) presents the ACI concept; (6) demonstrates the approach by presenting a comparison of different GS architectures; and (7) presents a discussion on the benefits of using this approach with a focus on commonality.

  4. A Framework for Developing Secure Application in Service – Oriented Architecture

    Directory of Open Access Journals (Sweden)

    Fariba Roozbeh

    Full Text Available Service Oriented Architecture (SOA is one of the most popular concepts to implement different systems. However it faces many challenges in terms of security. As a result, a number of standard and frame works are formed as supporters. The main purpose of ...

  5. Discussion enlightenment southwest Vernacular——Architecture Art Design development environment

    Institute of Scientific and Technical Information of China (English)

    薛柳毅

    2015-01-01

    Each area of residence due to the influence of the natural environment and human factors, showing a different design. Through the research of Southwest Nationalities architectural style, to explore their inner artistic design aesthetics, to better environmental art design and modern fusion.

  6. Cloning of gibberellin 3 beta-hydroxylase cDNA and analysis of endogenous gibberellins in the developing seeds in watermelon.

    Science.gov (United States)

    Kang, Hong-Gyu; Jun, Sung-Hoon; Kim, Joonyul; Kawaide, Hiroshi; Kamiya, Yuji; An, Gynheung

    2002-02-01

    We have isolated Cv3h, a cDNA clone from the developing seeds of watermelon, and have demonstrated significant amino acid homology with gibberellin (GA) 3 beta-hydroxylases. This cDNA clone was expressed in Escherichia coli as a fusion protein that oxidized GA(9) and GA(12) to GA(4) and GA(14), respectively. The Cv3h protein had the highest similarity with pumpkin GA 2 beta,3 beta-hydroxylase, but did not possess 2 beta-hydroxylation function. RNA blot analysis showed that the gene was expressed primarily in the inner parts of developing seeds, up to 10 d after pollination (DAP). In the parthenocarpic fruits induced by treatment with 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU), the embryo and endosperm of the seeds were undeveloped, whereas the integumental tissues, of maternal origin, showed nearly normal development. Cv3h mRNA was undetectable in the seeds of CPPU-treated fruits, indicating that the GA 3 beta-hydroxylase gene was expressed in zygotic cells. In our analysis of endogenous GAs from developing seeds, GA(9) and GA(4) were detected at high levels but those of GA(20) and GA(1) were very low. This demonstrates that GA biosynthesis in seeds prefers a non-13-hydroxylation pathway over an early 13-hydroxylation pathway. We also analyzed endogenous GAs from seeds of the parthenocarpic fruits. The level of bioactive GA(4 )was much lower there than in normal seeds, indicating that bioactive GAs, unconnected with Cv3h, exist in integumental tissues during early seed development. PMID:11867694

  7. Self-Assembled Multifunctional Hybrids: Toward Developing High-Performance Graphene-Based Architectures for Energy Storage Devices

    OpenAIRE

    Islam, Md Monirul; Aboutalebi, Seyed Hamed; Cardillo, Dean; Liu, Hua Kun; Konstantinov, Konstantin; Dou, Shi Xue

    2015-01-01

    The prospect of developing multifunctional flexible three-dimensional (3D) architectures based on integrative chemistry for lightweight, foldable, yet robust, electronic components that can turn the many promises of graphene-based devices into reality is an exciting direction that has yet to be explored. Herein, inspired by nature, we demonstrate that through a simple, yet novel solvophobic self-assembly processing approach, nacre-mimicking, layer-by-layer grown, hybrid composite materials (c...

  8. Defective Peripheral Nerve Development Is Linked to Abnormal Architecture and Metabolic Activity of Adipose Tissue in Nscl-2 Mutant Mice

    OpenAIRE

    Karen Ruschke; Henning Ebelt; Nora Klöting; Thomas Boettger; Kay Raum; Matthias Blüher; Thomas Braun

    2009-01-01

    BACKGROUND: In mammals the interplay between the peripheral nervous system (PNS) and adipose tissue is widely unexplored. We have employed mice, which develop an adult onset of obesity due to the lack the neuronal specific transcription factor Nscl-2 to investigate the interplay between the nervous system and white adipose tissue (WAT). METHODOLOGY: Changes in the architecture and innervation of WAT were compared between wildtype, Nscl2-/-, ob/ob and Nscl2-/-//ob/ob mice using morphological m...

  9. Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture

    OpenAIRE

    McCall, A. Scott; Cummings, Christopher F.; Bhave, Gautam; Vanacore, Roberto; Page-McCaw, Andrea; Hudson, Billy G.

    2014-01-01

    Bromine is ubiquitously present in animals as ionic bromide (Br−) yet has no known essential function. Herein, we demonstrate that Br− is a required cofactor for peroxidasin-catalyzed formation of sulfilimine crosslinks, a post-translational modification essential for tissue development and architecture found within the collagen IV scaffold of basement membranes (BMs). Bromide, converted to hypobromous acid, forms a bromosulfonium-ion intermediate that energetically selects for sulfilimine fo...

  10. Rapid Development of Adaptive, Climate-Driven Clinal Variation in Seed Mass in the Invasive Annual Forb Echium plantagineum L.

    OpenAIRE

    Konarzewski, Tara K.; Murray, Brad R.; Godfree, Robert C.

    2012-01-01

    We examined adaptive clinal variation in seed mass among populations of an invasive annual species, Echium plantagineum, in response to climatic selection. We collected seeds from 34 field populations from a 1,000 km long temperature and rainfall gradient across the species' introduced range in south-eastern Australia. Seeds were germinated, grown to reproductive age under common glasshouse conditions, and progeny seeds were harvested and weighed. Analyses showed that seed mass was significan...

  11. Seeds of confusion : the impact of policies on seed systems

    NARCIS (Netherlands)

    Louwaars, N.P.

    2007-01-01

    Seed is basic to crop production. Next to its importance in production, food security and rural development, seed is a key element in many debates about technology development and transfer, biodiversity, globalisation and equity. The sustainable availability of good quality seed is thus an important

  12. Perceived benefits from enterprise architecture

    OpenAIRE

    Plessius, Henk; Steenbergen, Marlies van; Slot, Raymond

    2014-01-01

    Enterprise Architecture has been developed in order to optimize the alignment between business needs and the (rapidly changing) possibilities of information technology. But do organizations indeed benefit from the application of Enterprise Architecture according to those who are in any way involved in architecture? To answer this question, a model has been developed (the Enterprise Architecture Value Framework) to organize the benefits of Enterprise Architecture. Based on this model, a survey...

  13. The effect of traditional architecture elements on architectureal and planning forming develop and raise the efficency of using the traditional energy (study case Crater/Aden, Yemen)

    International Nuclear Information System (INIS)

    This paper discuss the role of architecture in Center city-Aden, Republic of Yemen which has a historical traditional architecture which is a unique sample with many elements that make the building of this city as an effective helper in keeping the sources traditional energy. This architecture could be meritoriously described as courtyards, high ceiling for suitable air circling are used as well as the main building material used are local and environmental such as stones, wood and lime stone (Pumic). The research aim at studying and analyzing the planning forming and architectural specification of this city through studying some examples of its buildings to recognize the traditional building role in saving the traditional energy by studying the building material, ventilation system, orientation and opening, for using these elements to raise the efficiency of using the resources of traditional sources. The research is abbreviated to several results such as: 1. Urbanization planning side: a. Elements of urban planning represented in the mass and opening their environmental role. b. Method of forming the urban planning. c. Series in arrangement of elements of urban planning. 2. Architectural side: a. Ratio between solid and void. b. opening shapes. c. internal courtyards. d. Unique architectural elements (Mashrabiyas (Oriels), sky lines, opening covering...etc). e. Building material used . f. building construction methods. g. Kind of walls.(Author)

  14. Symbiotic in vitro seed propagation of Dendrobium: fungal and bacterial partners and their influence on plant growth and development.

    Science.gov (United States)

    Teixeira da Silva, Jaime A; Tsavkelova, Elena A; Zeng, Songjun; Ng, Tzi Bun; Parthibhan, S; Dobránszki, Judit; Cardoso, Jean Carlos; Rao, M V

    2015-07-01

    The genus Dendrobium is one of the largest genera of the Orchidaceae Juss. family, although some of its members are the most threatened today. The reason why many species face a vulnerable or endangered status is primarily because of anthropogenic interference in natural habitats and commercial overexploitation. The development and application of modern techniques and strategies directed towards in vitro propagation of orchids not only increases their number but also provides a viable means to conserve plants in an artificial environment, both in vitro and ex vitro, thus providing material for reintroduction. Dendrobium seed germination and propagation are challenging processes in vivo and in vitro, especially when the extreme specialization of these plants is considered: (1) their biotic relationships with pollinators and mycorrhizae; (2) adaptation to epiphytic or lithophytic life-styles; (3) fine-scale requirements for an optimal combination of nutrients, light, temperature, and pH. This review also aims to summarize the available data on symbiotic in vitro Dendrobium seed germination. The influence of abiotic factors as well as composition and amounts of different exogenous nutrient substances is examined. With a view to better understanding how to optimize and control in vitro symbiotic associations, a part of the review describes the strong biotic relations of Dendrobium with different associative microorganisms that form microbial communities with adult plants, and also influence symbiotic seed germination. The beneficial role of plant growth-promoting bacteria is also discussed. PMID:25940846

  15. Seed germination and seedling development in response to submergence in tree species of the Central Amazonian floodplains.

    Science.gov (United States)

    de Melo, Risolandia Bezerra; Franco, Augusto César; Silva, Clovis Oliveira; Piedade, Maria Teresa Fernandez; Ferreira, Cristiane Silva

    2015-01-01

    Successful germination and seedling establishment are crucial steps for maintenance and expansion of plant populations and recovery from perturbations. Every year the Amazon River and its tributaries overflow and flood the adjacent forest, exerting a strong selective pressure on traits related to seedling recruitment. We examined seed characteristics, stored reserves, germination, seedling development and survival under water of eight representative tree species from the lower portions of the flood-level gradient to identify adaptive strategies that contribute to their regeneration in this extreme ecosystem. Submerged seedlings were assessed for longevity and survival until they showed symptoms of injury. At this point, the remaining healthy seedlings were planted in unsaturated soil to monitor recovery after re-exposure to air over 30 days. All small (seed mass ≤0.17 g) seeds had epigeal phanerocotylar-type germination, a trait that would allow plants to acquire light and CO2 in the shortest time. Cell wall storage polysaccharide was a major component of all seeds, suggesting plant investment in structural reserves. Seven of the eight species germinated and formed healthy seedlings under water that endured submersion without any apparent injury for periods of 20-115 days, depending on the species. Seedlings of some species changed the direction of root growth and grew towards the surface of the water, which might have increased the uptake of oxygen to the tissues. Only one of the seven species did not survive re-exposure to air. Species able to germinate and produce seedlings under submersion, which subsequently are able to establish in aerated soils, would have more time available for terrestrial growth. This is critical for colonization of lower portions of the flood-level gradient where establishment is constrained by the short terrestrial phase that precedes the next flood. PMID:25922297

  16. Starch Granule and Protein Accumulation during Seed Development of Ginkgo biloba L.

    OpenAIRE

    Jin, Biao; Xie, Yan; Lu, Yan; Wang, Di; Zhang, Min; Wang, Li

    2012-01-01

    We investigated starch and protein formation and accumulation in the seed of Ginkgo biloba L. In the testa, starch granules and protein bodies (PBs) started to form and accumulate 30 days after pollination; they decreased in size and completely disappeared before maturity. In the endosperm, starch granules began to accumulate 45 days after pollination, and the number and size of starch granules increased gradually within 65 days after pollination. Starch granules, which were mainly produced i...

  17. Effects of Soil Substrate Contaminated by Knotweed Leaves on Seed Development

    Czech Academy of Sciences Publication Activity Database

    Šerá, Božena

    2012-01-01

    Roč. 21, č. 3 (2012), s. 713-717. ISSN 1230-1485 R&D Projects: GA MŠk OC10032; GA MZe QH72117 Institutional research plan: CEZ:AV0Z60870520 Keywords : seed germination * early growth * hormesis * allelopathy * phytotoxicity * Reynoutria * biotechnology Subject RIV: DK - Soil Contamination ; De-contamination incl. Pesticides Impact factor: 0.462, year: 2012

  18. Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

    OpenAIRE

    Zhawar, Vikramjit Kaur; Kaur, Narinder; Gupta, Anil Kumar

    2011-01-01

    Phytic acid and raffinose series oligosaccharides (RFOs) have anti-nutritional properties where phytic acid chelates minerals and reduces their bioavailability to humans and other animals, and RFOs cause flatulence. Both phytic acid and RFOs cannot be digested by monogastric animals and are released as pollutant-wastes. Efforts are being made to reduce the contents of these factors without affecting the viability of seeds. This will require a thorough understanding of their metabolism in diff...

  19. Study on Development of Non-Destructive Measurement Technique for Viability of Lettuce Seed (Lactuca sativa L) Using Hyperspectral Reflectance Imaging

    International Nuclear Information System (INIS)

    In this study, the feasibility of hyperspectral reflectance imaging technique was investigated for the discrimination of viable and non-viable lettuce seeds. The spectral data of hyperspectral reflectance images with the spectral range between 750 nm and 1000 nm were used to develop PLS-DA model for the classification of viable and non-viable lettuce seeds. The discrimination accuracy of the calibration set was 81.6% and that of the test set was 81.2%. The image analysis method was developed to construct the discriminant images of non-viable seeds with the developed PLS-DA model. The discrimination accuracy obtained from the resultant image were 91%, which showed the feasibility of hyperspectral reflectance imaging technique for the mass discrimination of non-viable lettuce seeds from viable ones.

  20. Study on Development of Non-Destructive Measurement Technique for Viability of Lettuce Seed (Lactuca sativa L) Using Hyperspectral Reflectance Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Chi Kook; Cho, Byoung Kwan [College of Agriculture and Life Science, Chungnam National University, Daejeon (Korea, Republic of); Mo, Chang Yeon [National Acadamy of Agricultural Science, Daejeon (Korea, Republic of); Kim, Moon S. [Environmental Microbial and Food Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Washington (United States)

    2012-10-15

    In this study, the feasibility of hyperspectral reflectance imaging technique was investigated for the discrimination of viable and non-viable lettuce seeds. The spectral data of hyperspectral reflectance images with the spectral range between 750 nm and 1000 nm were used to develop PLS-DA model for the classification of viable and non-viable lettuce seeds. The discrimination accuracy of the calibration set was 81.6% and that of the test set was 81.2%. The image analysis method was developed to construct the discriminant images of non-viable seeds with the developed PLS-DA model. The discrimination accuracy obtained from the resultant image were 91%, which showed the feasibility of hyperspectral reflectance imaging technique for the mass discrimination of non-viable lettuce seeds from viable ones.

  1. Three-dimensional architecture and development of Danianbryozoan mounds at Limhamn, south-west Sweden, usingground-penetrating radar

    DEFF Research Database (Denmark)

    Nielsen, Lars; Schack von Brockdorff, A.; Bjerager, Morten Gustav Erik;

    2009-01-01

    in the Limhamn limestone quarry, south-west Sweden, obtained from combined reflected ground-penetrating radar signals and outcrop analysis provide new information about the architecture and growth development of such mounds. The mounds are composed of bryozoan limestone and dark-grey to black flint...... the internal layering of the mounds which, typically, have widths and lengths of 30 to 60 m and heights of 5 to 10 m. Mound architecture and growth show great variability in the ground-penetrating radar images. Small-scale mound structures with a palaeorelief of only a few metres may constitute the...... during growth, although some mound structures indicate a preferential growth direction towards the south. Growth patterns interpreted from the ground-penetrating radar images suggest that the palaeocurrents in the study area may have shown great variability, even on a small scale. This observation is in...

  2. Space station data system analysis/architecture study. Task 2: Options development DR-5. Volume 1: Technology options

    Science.gov (United States)

    1985-01-01

    The second task in the Space Station Data System (SSDS) Analysis/Architecture Study is the development of an information base that will support the conduct of trade studies and provide sufficient data to make key design/programmatic decisions. This volume identifies the preferred options in the technology category and characterizes these options with respect to performance attributes, constraints, cost, and risk. The technology category includes advanced materials, processes, and techniques that can be used to enhance the implementation of SSDS design structures. The specific areas discussed are mass storage, including space and round on-line storage and off-line storage; man/machine interface; data processing hardware, including flight computers and advanced/fault tolerant computer architectures; and software, including data compression algorithms, on-board high level languages, and software tools. Also discussed are artificial intelligence applications and hard-wire communications.

  3. Generation and Development of RNA Ligase Ribozymes with Modular Architecture Through “Design and Selection”

    Directory of Open Access Journals (Sweden)

    Yuki Fujita

    2010-08-01

    Full Text Available In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules. Rational RNA design is an attractive alternative to avoid this laborious step, but rational design of catalytic modules is still a challenging task. A hybrid strategy of in vitro selection and rational design has been proposed. With this strategy termed “design and selection,” new ribozymes can be generated through installation of catalytic modules onto RNA scaffolds with defined 3D structures. This approach, the concept of which was inspired by the modular architecture of naturally occurring ribozymes, allows prediction of the overall architectures of the resulting ribozymes, and the structural modularity of the resulting ribozymes allows modification of their structures and functions. In this review, we summarize the design, generation, properties, and engineering of four classes of ligase ribozyme generated by design and selection.

  4. Sustainable Tourism Development Through Sustainable Architecture: A Projected Case Study of Cox’s Bazar, Bangladesh

    OpenAIRE

    Sharma, Krishna

    2011-01-01

    ‘The problems of environmental damage, economic unsustainability and social dislocation caused by rapid and extensive places for recreation growth might suggest that the key to greater sustainability lies in setting limits to urbanization and changing the rules in architecture and planning for tourism.’ (Delia, 2005, pp. 98-10). The very essence of above quotation has an inseparable relation with the research and therefore the study does fix its primary and principal aim to acquire know...

  5. Developing a Framework for Evaluating Service Oriented Architecture Governance with Approach COBIT

    OpenAIRE

    EMADI, Sima; DEHGHANI, Mojgan

    2015-01-01

    Todays, organizations need an effective governance framework for their service-oriented architecture (SOA) so that they can use the framework to evaluate their current state governance, determine the requirements of governance, and then provide a suitable model for their governance. So far, various frameworks to evaluate the governance of SOA are presented. In this paper, after a brief introduction of the internal control framework COBIT, in order to provide a framework for evaluating SOA gov...

  6. The Interaction Between Landscape Architecture and Urban Development. Do we Have a Common Goal?

    Directory of Open Access Journals (Sweden)

    Vaiva Deveikienė

    2015-05-01

    Full Text Available The article analyses the problem of the relationship and interaction between urban design and landscape architecture. This refers to the period of the modern city from the late nineteenth century to the present day. There are presented and discussed urbanization processes and examples of solutions with emphasis on problems arising from the relationship between a city and nature as well as those related to urban landscape and sustainability of urban landscaping in the twentieth century.

  7. Bioclimatic Architecture, a Sensible and Logical Approach towards the Future of Building Development

    OpenAIRE

    Ţundrea, Horia; Budescu, Mihai

    2013-01-01

    The modern world building scene tends to depend more and more on the economical climate as well as the geographical climate itself, as none of the major players in the construction game can afford to ignore current conditions and dismiss logical solutions that can drastically cut the impact on the environment as well as the exploitation costs. The present paper will provide the information needed to understand the bioclimatic architecture concept, as well as describe a few examples of such ap...

  8. Architecture, Control and NVH Development of Digital Hydraulics for Off-Highway Vehicle Applications

    OpenAIRE

    Yuan, QingHui; Jogada, Aaron

    2016-01-01

    Digital hydraulics is one of promising technologies having a huge potential to significantly improve energy efficiency in the fluid power industry. In this paper, we present a digital hydraulics solution for mobile market with a large ammount of energy usage by hydraulic components and systems. Specifically, a novel hydraulic architecture, Multiplex Digital Valve (MDV) system that employs digital valves to meet multiple service pressure/flow requirement in off highway vehicles, is introduced....

  9. Development of an Extended Product Lifecycle Management through Service Oriented Architecture.

    OpenAIRE

    Cassina, J.; Cannata, A; TAISCH, M.

    2009-01-01

    The aim of this work is to define new business opportunities through the concept of Extended Product Lifecycle Management (ExtPLM), analysing its potential implementation within a Service Oriented Architecture. ExtPLM merges the concepts of Extended Product, Avatar and PLM. It aims at allowing a closer interaction between enterprises and their customers, who are integrated in all phases of the life cycle, creating new technical functionalities and services, improving both the prac...

  10. Review and Perspective of Architecture Development for Dynamic Random Access Memory

    Institute of Scientific and Technical Information of China (English)

    WANG Yu-xing; WU Jin

    2008-01-01

    Discussed is a review and perspective of architecture, materials and process technology for dynamic random access memory(DRAM) applications. Key challenges of the transistor and capacitor scaling from DRAM will be reviewed. To continue scaling down, multi-gate devices with very thin silicon channels are most promising. Several architectures like Fin-field effect transistor(Fin-FET), Wafer bonded double gate and silicon on nothing(SON) gate-all-around have been demonstrated with good electrical characteristics. An overview of the evolution of capacitor technology is also presented from the early days of planar poly/insulator/silicon(PIS) capacitors to the metal/insulator/metal(MIM) capacitors used for today 50 nm technology node and below. In comparing Ta2O5, HfO2 and Al2O3 as high-k dielectric for use in DRAM technology, Al2O3 is found to give a good compromise between capacitor performance and manufacturability used in MIM architecture.

  11. Differentially Accumulated Proteins in Coffea arabica Seeds during Perisperm Tissue Development and Their Relationship to Coffee Grain Size.

    Science.gov (United States)

    Alves, Leonardo Cardoso; Magalhães, Diogo Maciel De; Labate, Mônica Teresa Veneziano; Guidetti-Gonzalez, Simone; Labate, Carlos Alberto; Domingues, Douglas Silva; Sera, Tumoru; Vieira, Luiz Gonzaga Esteves; Pereira, Luiz Filipe Protasio

    2016-02-24

    Coffee is one of the most important crops for developing countries. Coffee classification for trading is related to several factors, including grain size. Larger grains have higher market value then smaller ones. Coffee grain size is determined by the development of the perisperm, a transient tissue with a highly active metabolism, which is replaced by the endosperm during seed development. In this study, a proteomics approach was used to identify differentially accumulated proteins during perisperm development in two genotypes with regular (IPR59) and large grain sizes (IPR59-Graudo) in three developmental stages. Twenty-four spots were identified by MALDI-TOF/TOF-MS, corresponding to 15 proteins. We grouped them into categories as follows: storage (11S), methionine metabolism, cell division and elongation, metabolic processes (mainly redox), and energy. Our data enabled us to show that perisperm metabolism in IPR59 occurs at a higher rate than in IPR59-Graudo, which is supported by the accumulation of energy and detoxification-related proteins. We hypothesized that grain and fruit size divergences between the two coffee genotypes may be due to the comparatively earlier triggering of seed development processes in IPR59. We also demonstrated for the first time that the 11S protein is accumulated in the coffee perisperm. PMID:26809209

  12. Architecture and Stages

    DEFF Research Database (Denmark)

    Kiib, Hans

    2009-01-01

    future. But this is not enough. The agenda is to develop architectural spaces, where social interaction and learning are enhanced by art and fun. How can we develop new architectural designs in our inner cities and waterfronts where eventscapes, learning labs and temporal use are merged with everyday......Architecture and Art as Fuel New development zones for shopping and entertainment and space for festivals inside the city CAN be coupled with art and architecture and become ‘open minded' public domains based on cultural exchange and mutual learning. This type of space could be labelled as...... "experiencescape" - a space between tourism, culture, learning and economy. Strategies related to these challenges involve new architectural concepts and art as ‘engines' for a change. New expressive architecture and old industrial buildings are often combined into hybrid narratives, linking the past with the...

  13. Data-Centric Enterprise Architecture

    Directory of Open Access Journals (Sweden)

    Zeinab Rajabi

    2012-08-01

    Full Text Available Enterprises choose Enterprise Architecture (EA solution, in order to overcome dynamic business challenges and in coordinate various enterprise elements. In this article, a solution is suggested for the Enterprise Architecture development. The solution focuses on architecture data in the Enterprise Architecture development process. Data-centric architecture approach is preferred product-centric architecture approach. We suggest using Enterprise Ontology (EO as context for collecting architecture data; Enterprise Ontology enhances quality of architecture data and lead to effective architecture results for decision-making. First, Enterprise is modeled using the ontology. Then how collecting Enterprise Architecture data based on the Enterprise Ontology is explained. Finally, the results and advantages of the solution are demonstrated.

  14. Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum: An orchid with an extremely small population in China

    Directory of Open Access Journals (Sweden)

    Ying Chen

    2015-01-01

    Full Text Available Paphiopedilum spicerianum  is listed as one of the country’s Wild Plants with Extremely Small Populations (PSESP. Procedures were developed for asymbiotic seed germination and seedling development aimed at producing seedlings for reintroduction. The highest germination was achieved in RECW with a 24 h dark cycle after pretreatment with 1% NaOCl for 40 min after 30 days from germination. However, these protocorms remained white and did not develop further. Although germination was lower under the same conditions in MSCW, it resulted in healthier and greener protocorms. Of four suitable media tested to promote seedling formation, Hyponex No 1 medium with 1.0mgl−1α-naphthalene acetic acid, 0.5gl−1 activated charcoal and 10% banana homogenate was the most effective. Advanced seedling development was seen in all six tested media during a 4 month growing period, with the highest leaf growth rate seen in the same media used for seedling formation, supplemented with 1.0mgl−16-benzyladenine added to promote leaf growth. Fluorescein diacetate (FDA tests on seeds showed that higher salt concentrations in the medium and longer duration of exposure to NaOCl reduce germination because of damaging effects on the testa and the embryo cells.

  15. Differential seed handling by two African primates affects seed fate and establishment of large-seeded trees

    Science.gov (United States)

    Gross-Camp, Nicole D.; Kaplin, Beth A.

    2011-11-01

    fate, and may be helpful in developing models to predict seed shadows and recruitment patterns of large-seeded trees.

  16. Muscle architecture during the course of development of Diplostomum pseudospathaceum Niewiadomska, 1984 (Trematoda, Diplostomidae) from cercariae to metacercariae.

    Science.gov (United States)

    Petrov, A; Podvyaznaya, I

    2016-05-01

    Recent confocal microscopy studies have greatly expanded our knowledge of muscle systems in cercariae and adult digeneans, but the gross anatomy and development of metacercarial musculature remain relatively little known. To further our understanding of metacercarial development, this study used phalloidin staining and confocal microscopy to examine changes in muscle architecture over the course of development from cercariae to infective metacercariae in Diplostomum pseudospathaceum Niewiadomska, 1984. The paper describes muscle development in the body wall, anterior organ (oral sucker), acetabulum, pharynx and midgut and in the musculo-glandular organs that first appear in metacercariae (lappets and holdfast). The muscle architecture of the cercarial tail is also described. The results of the study support previously reported observations that diplostomid musculature undergoes substantial transformation during metacercarial development. The most profound changes, involving extensive remodelling and replacement of cercarial muscles, were seen in the body-wall musculature and in the anterior organ as it developed into the oral sucker. Muscle systems of other cercarial organs showed more gradual changes. The adaptive importance of developmental changes in musculature is discussed. PMID:25997697

  17. Control of Microstructural Development in Reaction—Bonded Silicon Nitride(RBSN) by Seeding With β—Si3N4 Single Crystals

    Institute of Scientific and Technical Information of China (English)

    K.Amoako-Appiagyei; ByungSeiJun; 等

    1998-01-01

    The addition of β-Si3N4 seed grains to a ceramic powder is an effective means of controlling the microstructural evolution,In this work addition of β-Si3N4 seed grains gave rise to a bi-modal microstructure made up of large rod-lkike grains that grew from the added seed during the liquid phase sintering process and the usua elongated grains which are developed from the α→β transformation process in silicon nitride,The morphology of te grains that were grown epitaxially from the seed was influenced by the size and shape of the seed crystals;the relatively large and elongated seed grains gave rise to large rod-like grains whilst those with equiaxed-like shape gave rise to large grains similar in shape to the seed from which they were grown.The evolution of the large rod-like grains and the resultant bi-modal microstructure led to a considerable improvement in the mechanical properties.

  18. Integration of system design and production processes in robust mechatronic product architectures development - extended M-FBFP framework

    Directory of Open Access Journals (Sweden)

    Osman Krešimir

    2013-01-01

    Full Text Available The complexity of mechatronic products such as climate chamber subsystems results in enormous difficulties in understanding where the main design process inefficiencies are. It is therefore extremely difficult to determine which improvements will have the most significant impact on a company or on a specific project. Mechatronic products are characterized by a high level of interdisciplinarity and complexity in the technical system and the relevant development processes. The main challenge in this respect is how to deal with the high complexity of and a variety of interdependencies in such products. We are therefore presenting a framework for integrated mechatronic product and process modelling - extended M-FBFP framework. This framework provides different independent perspectives of the overall product to improve their architecture. As a result of the proposed framework, risk analysis through subsystems in the components domain and through processes in the technical processes domain is enabled and it is now possible to provide feedback on product architecture. To obtain optimally robust product architectures from available alternative solutions, an evaluation analysis was performed across all stages, including the initialization and subsequent refinements with several evaluation criteria: complexity, interdependency and process duration. To test the validity of the proposed framework, we are presenting a case study involving a climate chamber with heat regeneration.

  19. Development of imaging and reconstructions algorithms on parallel processing architectures for applications in non-destructive testing

    International Nuclear Information System (INIS)

    This thesis work is placed between the scientific domain of ultrasound non-destructive testing and algorithm-architecture adequation. Ultrasound non-destructive testing includes a group of analysis techniques used in science and industry to evaluate the properties of a material, component, or system without causing damage. In order to characterise possible defects, determining their position, size and shape, imaging and reconstruction tools have been developed at CEA-LIST, within the CIVA software platform. Evolution of acquisition sensors implies a continuous growth of datasets and consequently more and more computing power is needed to maintain interactive reconstructions. General purpose processors (GPP) evolving towards parallelism and emerging architectures such as GPU allow large acceleration possibilities than can be applied to these algorithms. The main goal of the thesis is to evaluate the acceleration than can be obtained for two reconstruction algorithms on these architectures. These two algorithms differ in their parallelization scheme. The first one can be properly parallelized on GPP whereas on GPU, an intensive use of atomic instructions is required. Within the second algorithm, parallelism is easier to express, but loop ordering on GPP, as well as thread scheduling and a good use of shared memory on GPU are necessary in order to obtain efficient results. Different API or libraries, such as OpenMP, CUDA and OpenCL are evaluated through chosen benchmarks. An integration of both algorithms in the CIVA software platform is proposed and different issues related to code maintenance and durability are discussed. (author)

  20. THE DESIGN OF MOSQUES AS COMMUNITY DEVELOPMENT CENTERS FROM THE PERSPECTIVE OF THE SUNNA AND WRIGHT’S ORGANIC ARCHITECTURE

    Directory of Open Access Journals (Sweden)

    Mohamad Tajuddin Mohamad Rasdi, Nangkula Utaberta

    2012-03-01

    Full Text Available The main purpose of this paper is to present an alternative approach to mosque design with particular reference to the context of Malaysia and the Malay World or the Nusantara. The paper contains four main parts. The first part examines the problem of mosque architectural interpretation from the perspective of Western architectural historiography. The main problems highlighted are those which pertain to the idea of ‘architecture’, ‘religious architecture’, relevance of the body of knowledge known as the ‘Hadith’ and lastly the specific historical-political agenda of some traditional mosques. The second part of the paper expounds briefly a reinterpretation of the hadith in arguing that the mosque approaches more as a community development complex than that as a mere house of rituals. The third part of the paper examines Wright’s Organic Architecture with specific reference to the design of the Unity Church and a few other buildings as containing some important lessons of mosque designs from the perspective of the Sunnah. The final part of the paper uses the principles discussed to criticize the Putra Mosque and the Masjid Negara.  

  1. Rapid EHR development and implementation using web and cloud-based architecture in a large home health and hospice organization.

    Science.gov (United States)

    Weaver, Charlotte A; Teenier, Pamela

    2014-01-01

    Health care organizations have long been limited to a small number of major vendors in their selection of an electronic health record (EHR) system in the national and international marketplace. These major EHR vendors have in common base systems that are decades old, are built in antiquated programming languages, use outdated server architecture, and are based on inflexible data models [1,2]. The option to upgrade their technology to keep pace with the power of new web-based architecture, programming tools and cloud servers is not easily undertaken due to large client bases, development costs and risk [3]. This paper presents the decade-long efforts of a large national provider of home health and hospice care to select an EHR product, failing that to build their own and failing that initiative to go back into the market in 2012. The decade time delay had allowed new technologies and more nimble vendors to enter the market. Partnering with a new start-up company doing web and cloud based architecture for the home health and hospice market, made it possible to build, test and implement an operational and point of care system in 264 home health locations across 40 states and three time zones in the United States. This option of "starting over" with the new web and cloud technologies may be posing a next generation of new EHR vendors that retells the Blackberry replacement by iPhone story in healthcare. PMID:24943570

  2. Accumulation of Phosphorus-Containing Compounds in Developing Seeds of Low-Phytate Pea (Pisum sativum L.) Mutants

    OpenAIRE

    Shunmugam, Arun S.K.; Cheryl Bock; Arganosa, Gene C.; Fawzy Georges; Gray, Gordon R.; Warkentin, Thomas D.

    2014-01-01

    Low phytic acid (lpa) crops are low in phytic acid and high in inorganic phosphorus (Pi). In this study, two lpa pea genotypes, 1-150-81, 1-2347-144, and their progenitor CDC Bronco were grown in field trials for two years. The lpa genotypes were lower in IP6 and higher in Pi when compared to CDC Bronco. The total P concentration was similar in lpa genotypes and CDC Bronco throughout the seed development. The action of myo-inositol phosphate synthase (MIPS) (EC 5.5.1.4) is the first and rate-...

  3. Development of a Subcell Based Modeling Approach for Modeling the Architecturally Dependent Impact Response of Triaxially Braided Polymer Matrix Composites

    Science.gov (United States)

    Sorini, Chris; Chattopadhyay, Aditi; Goldberg, Robert K.; Kohlman, Lee W.

    2016-01-01

    Understanding the high velocity impact response of polymer matrix composites with complex architectures is critical to many aerospace applications, including engine fan blade containment systems where the structure must be able to completely contain fan blades in the event of a blade-out. Despite the benefits offered by these materials, the complex nature of textile composites presents a significant challenge for the prediction of deformation and damage under both quasi-static and impact loading conditions. The relatively large mesoscale repeating unit cell (in comparison to the size of structural components) causes the material to behave like a structure rather than a homogeneous material. Impact experiments conducted at NASA Glenn Research Center have shown the damage patterns to be a function of the underlying material architecture. Traditional computational techniques that involve modeling these materials using smeared homogeneous, orthotropic material properties at the macroscale result in simulated damage patterns that are a function of the structural geometry, but not the material architecture. In order to preserve heterogeneity at the highest length scale in a robust yet computationally efficient manner, and capture the architecturally dependent damage patterns, a previously-developed subcell modeling approach where the braided composite unit cell is approximated as a series of four adjacent laminated composites is utilized. This work discusses the implementation of the subcell methodology into the commercial transient dynamic finite element code LS-DYNA (Livermore Software Technology Corp.). Verification and validation studies are also presented, including simulation of the tensile response of straight-sided and notched quasi-static coupons composed of a T700/PR520 triaxially braided [0deg/60deg/-60deg] composite. Based on the results of the verification and validation studies, advantages and limitations of the methodology as well as plans for future work

  4. Development of bioartificial myocardium by electrostimulation of 3D collagen scaffolds seeded with stem cells

    Directory of Open Access Journals (Sweden)

    Alain Carpentier

    2012-06-01

    Full Text Available Electrostimulation (ES can be defined as a safe physical method to induce stem cell differentiation. The aim of this study is to evaluate the effectiveness of ES on bone marrow mesenchymal stem cells (BMSCs seeded in collagen scaffolds in terms of proliferation and differentiation into cardiomyocytes. BMSCs were isolated from Wistar rats and seeded into 3D collagen type 1 templates measuring 25 x 25 x 6 mm. Bipolar in vitro ES was performed during 21 days. Electrical impedance and cell proliferation were measured. Expression of cardiac markers was assessed by immunocytochemistry. Viscoelasticity of collagen matrix was evaluated. Electrical impedance assessments showed a low resistance of 234±41 Ohms which indicates good electrical conductivity of collagen matrix. Cell proliferation at 570 nm as significantly increased in ES groups after seven day (ES 0.129±0.03 vs non-stimulated control matrix 0.06±0.01, P=0.002 and after 21 days, (ES 0.22±0.04 vs control 0.13±0.01, P=0.01. Immunocytochemistry of BMSCs after 21 days ES showed positive staining of cardiac markers, troponin I, connexin 43, sarcomeric alpha-actinin, slow myosin, fast myosin and desmin. Staining for BMSCs marker CD29 after 21 days was negative. Electrostimulation of cell-seeded collagen matrix changed stem cell morphology and bio- chemical characteristics, increasing the expression of cardiac markers. Thus, MSC-derived differentiated cells by electrostimulation grafted in biological scaffolds might result in a convenient tissue engineering source for myocardial diseases.

  5. Seed proteomics

    Science.gov (United States)

    Seeds comprise a protective covering, a small embryonic plant, and a nutrient-storage organ. Seeds are protein-rich, and have been the subject of many mass spectrometry-based analyses. Seed storage proteins (SSP), which are transient depots for reduced nitrogen, have been studied for decades by cel...

  6. EVALUATING THE DISCONTINUED TRADITIONS OF MALAY WOOD CARVINGS IN MALAYSIA: A FAILURE TO DEVELOP THE DISCOURSE ON MODERN AND POST MODERN ORNAMENTATION IN ARCHITECTURAL WORKS

    Directory of Open Access Journals (Sweden)

    Nangkula Utaberta

    2014-01-01

    Full Text Available The main intention of this study is to contribute some thoughts on why the tradition of wood carvings in traditional Malay architecture was discontinued at the rise of modern and post-modern architectural works in Malaysia. Although the tradition of wood carvings more or less came to a stop in public architectural works with the development of colonial architecture, this study seeks to clarify the modernistic and post-modern arguments on the problem or issues of ornamentation. Although modernism mainly or seemingly rejected the use of ornaments, there was still a strong strand of its development in the architecture of Frank Lloyd Wright. The western post-modernist architects such as Michael Graves and Charles Jencks went on to develop a discourse on ornamentation under the area of architectural meaning and linguistics. It is expected that this critic paper can segragate and analyse issues and problems of Malay wood carvings and start a further discussion on the development of this traditional heritage which can contribute and reconstruct a better framework for the Architectural Identity of Malaysia.

  7. Heat recovery and seed recovery development project: preliminary design report (PDR)

    Energy Technology Data Exchange (ETDEWEB)

    Arkett, A. H.; Alexander, K. C.; Bolek, A. D.; Blackman, B. K.; Kurrle, P. E.; Tram, S. V.; Warren, A. M.; Ziobrowski, A. J.

    1981-06-01

    The preliminary design and performance characteristics are described of the 20 MWt heat recovery and seed recovery (HRSR) system to be fabricated, installed, and evaluated to provide a technological basis for the design of commercial size HRSR systems for coal-fired open-cycle MHD power plants. The system description and heat and material balances, equipment description and functional requirements, controls, interfacing systems, and operation and maintenance are detailed. Appendices include: (1) recommended environmental requirements for compliance with federal and state of Tennessee regulations, (2) channel and diffuser simulator, (3) equipment arrangement drawings, and (4) channel and diffuser simulator barrel drawings. (WHK)

  8. Effects of seed irradiation on the early development and mitochondrial RNA synthesis of 'Impala' barley

    International Nuclear Information System (INIS)

    The influence of fractionated γ-irradiation on barley seeds was investigated under outdoor and hothouse conditions. The doses were 250, 500, 1,000, and 2,000 rad. The resulting radiation effects were investigated from the point of view of molecular biology, i.e. studying the RNA synthesis of the mitochondria after 14C-labelling of uridine. The radiation influence on the length of the coleoptiles was another criterion. The irradiation findings are discussed in connection with the cultivation of better and more resistant plants for agriculture. (GSE/AK)

  9. ZEAXANTHIN EPOXIDASE Activity Potentiates Carotenoid Degradation in Maturing Seed.

    Science.gov (United States)

    Gonzalez-Jorge, Sabrina; Mehrshahi, Payam; Magallanes-Lundback, Maria; Lipka, Alexander E; Angelovici, Ruthie; Gore, Michael A; DellaPenna, Dean

    2016-07-01

    Elucidation of the carotenoid biosynthetic pathway has enabled altering the composition and content of carotenoids in various plants, but to achieve desired nutritional impacts, the genetic components regulating carotenoid homeostasis in seed, the plant organ consumed in greatest abundance, must be elucidated. We used a combination of linkage mapping, genome-wide association studies (GWAS), and pathway-level analysis to identify nine loci that impact the natural variation of seed carotenoids in Arabidopsis (Arabidopsis thaliana). ZEAXANTHIN EPOXIDASE (ZEP) was the major contributor to carotenoid composition, with mutants lacking ZEP activity showing a remarkable 6-fold increase in total seed carotenoids relative to the wild type. Natural variation in ZEP gene expression during seed development was identified as the underlying mechanism for fine-tuning carotenoid composition, stability, and ultimately content in Arabidopsis seed. We previously showed that two CAROTENOID CLEAVAGE DIOXYGENASE enzymes, CCD1 and CCD4, are the primary mediators of seed carotenoid degradation, and here we demonstrate that ZEP acts as an upstream control point of carotenoid homeostasis, with ZEP-mediated epoxidation targeting carotenoids for degradation by CCD enzymes. Finally, four of the nine loci/enzymatic activities identified as underlying natural variation in Arabidopsis seed carotenoids also were identified in a recent GWAS of maize (Zea mays) kernel carotenoid variation. This first comparison of the natural variation in seed carotenoids in monocots and dicots suggests a surprising overlap in the genetic architecture of these traits between the two lineages and provides a list of likely candidates to target for selecting seed carotenoid variation in other species. PMID:27208224

  10. Participatory tools working with crops, varieties and seeds. A guide for professionals applying participatory approaches in agrobiodiversity management, crop improvement and seed sector development

    NARCIS (Netherlands)

    Boef, de W.S.; Thijssen, M.H.

    2007-01-01

    Outline to the guide Within our training programmes on local management of agrobiodiversity, participatory crop improvement and the support of local seed supply participatory tools get ample attention. Tools are dealt with theoretically, are practised in class situations, but are also applied in fie

  11. Textile Architecture

    DEFF Research Database (Denmark)

    Heimdal, Elisabeth Jacobsen

    2010-01-01

    Textiles can be used as building skins, adding new aesthetic and functional qualities to architecture. Just like we as humans can put on a coat, buildings can also get dressed. Depending on our mood, or on the weather, we can change coat, and so can the building. But the idea of using textiles...... to create human habitation is not new. As Diether S. Hope phrases it, referring to tents: The history of development of humanity would be barely conceivable without free spanning textile membrane structures....

  12. Development of a Computer Architecture to Support the Optical Plume Anomaly Detection (OPAD) System

    Science.gov (United States)

    Katsinis, Constantine

    1996-01-01

    The NASA OPAD spectrometer system relies heavily on extensive software which repetitively extracts spectral information from the engine plume and reports the amounts of metals which are present in the plume. The development of this software is at a sufficiently advanced stage where it can be used in actual engine tests to provide valuable data on engine operation and health. This activity will continue and, in addition, the OPAD system is planned to be used in flight aboard space vehicles. The two implementations, test-stand and in-flight, may have some differing requirements. For example, the data stored during a test-stand experiment are much more extensive than in the in-flight case. In both cases though, the majority of the requirements are similar. New data from the spectrograph is generated at a rate of once every 0.5 sec or faster. All processing must be completed within this period of time to maintain real-time performance. Every 0.5 sec, the OPAD system must report the amounts of specific metals within the engine plume, given the spectral data. At present, the software in the OPAD system performs this function by solving the inverse problem. It uses powerful physics-based computational models (the SPECTRA code), which receive amounts of metals as inputs to produce the spectral data that would have been observed, had the same metal amounts been present in the engine plume. During the experiment, for every spectrum that is observed, an initial approximation is performed using neural networks to establish an initial metal composition which approximates as accurately as possible the real one. Then, using optimization techniques, the SPECTRA code is repetitively used to produce a fit to the data, by adjusting the metal input amounts until the produced spectrum matches the observed one to within a given level of tolerance. This iterative solution to the original problem of determining the metal composition in the plume requires a relatively long period of time

  13. Real-time objects development: Study and proposal for a parallel scheduling architecture

    International Nuclear Information System (INIS)

    This thesis contributes to the programming and the execution control of real-time object oriented applications. Using real-time objects is very interesting for programming real- time applications, because this model can introduce the concurrence with the encapsulation properties, with modularity and reusability by taking into account the real-time constraints of the application. One essential quality of this approach is that it can directly specify the parallelism and the real-time constraints at the model level of the application. An annotation system of C++ has been defined to describe the real-time specifications in the model (or in the source code) of the application. It will supply to the execution support the different information it needs for the control. In this approach of multitasking, the control is distributed and encapsulated inside each real time object. Three complementary levels of control have been defined: the state level (defining the capability of an object to treat an operation), the concurrence level (assuring the coherence between the object attributes) and a scheduling control (allocating the processors resources to the object by taking real-time constraints into account). The proposed control architecture, named OROS, manages the attribute access of each object in an individual way, then it can parallel treatments which do not access at the same data. This architecture makes a dynamic control of an application that can take benefit from the parallelism of the new machines both for the execution parallelism and the control itself. This architecture uses only the simplest primitives of the industrial real-time operating systems which ensures its feasibility and portability. (author)

  14. Efficacy of essential oils of Cymbopogon citratus (D.C. Stapf, Lippia multiflora Moldenke and hot water in the control of seed-borne fungi Phoma sorghina and their effects on Sorghum bicolor (L. Moench seed germination and plants development in Burkina Faso

    Directory of Open Access Journals (Sweden)

    Schémaeza BONZI1

    2013-12-01

    Full Text Available The effects of two essential oils (Cymbopogon citratus and Lippia multiflora and varying durations of hot water at 50°C on seed-borne fungi Phoma sorghina on five varieties of sorghum were evaluated using morphological traits. The varieties were ICSV 1001, Sariaso 03, ICSV 1049, Kapelga and the local variety, 1341So07. The results indicate that hot water treatment and essential oil of C. citratus significantly reduced the infection level of P. sorghina compared to untreated seeds, seeds treated with sterile water and fungicide Calthio C. Essential oil of L. multiflora, significantly lowered the infection level of P. sorghina, compared to untreated seeds and seeds treated with water in varieties of white grain such as Sariaso 03 and ICSV 1049. Hot water treatment did not have a repressive effect against sorghum seed germination for all the varieties tested. On the contrary, essential oil of C. citratus had an inhibitory effect on the germination of seeds of varieties Kapelga and ICSV 1049 compared to untreated seed. On the other varieties tested, it reduced the number of germinated seeds in comparison to untreated seed. According to tested varieties, essential oil of L. multiflora had an inhibitory effect (on ICSV 1049 or improved seed germination (on Kapelga. In general, the treatments applied have improved the development of sorghum plants grown from treated seed.

  15. Development of a Cs-seeded RF-driven H-ion source for the J-PARC

    International Nuclear Information System (INIS)

    A cesium (Cs) seeded RF-driven H-ion source using an internal-antenna developed at the SNS (Spallation Neutron Source) is under development for the J-PARC (Japan Proton Accelerator Research Complex). The requirements for the J-PARC second stage are an H-ion beam of 60mA within normalized emittances of 1.5μmm•mrad both horizontally and vertically, and a flat top beam duty factor of 1.25% (500μs x 25Hz). The RF-driven H-ion source was developed on the basis of a J-PARC ion source driven with a tungsten (W) filament. The axial magnetic field optimization by using an axial magnetic field compensation (AMFC) coil significantly enhances the H-ion beam by typically 10%. An H-ion beam with a flat top duty factor of 2.5% (1ms x 25Hz) of 77mA, whose about 90% (corresponding to about 70mA) of emittance of each direction is within 1.5πmm•mrad, is successfully extracted. During a demonstration operation over 100-hours, an H-ion beam over 66mA was successfully produced with a relatively low RF power of about 40kW by seeding Cs for 4-minutes a day. (author)

  16. Albendazole in environment: faecal concentrations in lambs and impact on lower development stages of helminths and seed germination.

    Science.gov (United States)

    Prchal, Lukáš; Podlipná, Radka; Lamka, Jiří; Dědková, Tereza; Skálová, Lenka; Vokřál, Ivan; Lecová, Lenka; Vaněk, Tomáš; Szotáková, Barbora

    2016-07-01

    Albendazole (ABZ), widely used benzimidazole anthelmintic, administered to animals enters via excrements into environment and may impact non-target organisms. Moreover, exposure of lower development stages of helminths to anthelmintics may also encourage the development of drug-resistant strains of helminths. In present project, the kinetics of ABZ (10 mg kg(-1) p.o.) and its metabolite (ABZ.SO, ABZSO2) elimination in faeces from treated Texel lambs were studied using UHPLC/MS/MS with the aim to find out their concentrations achievable in the environment. Consequently, the effect of these compounds on lower development stages of Barber's pole worm (Haemonchus contortus) and on germination of white mustard (Sinapis alba) seeds was evaluated. The results showed that ABZ concentrations in faeces excreted in 4-60 h after treatment were above the concentrations lethal for H. contortus eggs. Moreover, pre-incubation with sub-lethal doses of ABZ and ABZ.SO did not increase the resistance of H. contortus eggs and larvae to anthelmintics. On the other hand, concentrations of ABZ and ABZ.SO in faeces are so high that might have negative influence on non-target soil invertebrates. As neither ABZ nor its metabolites affect the germination of mustard seeds, phytoremediation could be considered as potential tool for detoxification of ABZ in the environment. PMID:26996913

  17. Role of Tec1 in the Development, Architecture, and Integrity of Sexual Biofilms of Candida albicans

    OpenAIRE

    Daniels, Karla J.; Srikantha, Thyagarajan; Pujol, Claude; Park, Yang-Nim; Soll, David R.

    2015-01-01

    MTL-homozygous (a/a or α/α) white cells form a complex sexual biofilm that exhibits the same architecture as that of MTL-heterozygous (a/α) pathogenic biofilms. However, the former is regulated by the mitogen-activated protein (MAP) kinase pathway, while the latter is regulated by the Ras1/cyclic AMP (cAMP) pathway. We previously demonstrated that in the formation of an MTL-homozygous, mature (48 h) sexual biofilm in RPMI 1640 medium, the MAP kinase pathway targets Tec1 rather than Cph1, the ...

  18. High light exposure on seed coat increases lipid accumulation in seeds of castor bean (Ricinus communis L.), a nongreen oilseed crop.

    Science.gov (United States)

    Zhang, Yang; Mulpuri, Sujatha; Liu, Aizhong

    2016-05-01

    Little was known on how sunlight affects the seed metabolism in nongreen seeds. Castor bean (Ricinus communis L.) is a typical nongreen oilseed crop and its seed oil is an important feedstock in industry. In this study, photosynthetic activity of seed coat tissues of castor bean in natural conditions was evaluated in comparison to shaded conditions. Our results indicate that exposure to high light enhances photosynthetic activity in seed coats and consequently increases oil accumulation. Consistent results were also reached using cultured seeds. High-throughput RNA-Seq analyses further revealed that genes involved in photosynthesis and carbon conversion in both the Calvin-Benson cycle and malate transport were differentially expressed between seeds cultured under light and dark conditions, implying several venues potentially contributing to light-enhanced lipid accumulation such as increased reducing power and CO2 refixation which underlie the overall lipid biosynthesis. This study demonstrated the effects of light exposure on oil accumulation in nongreen oilseeds and greatly expands our understanding of the physiological roles that light may play during seed development in nongreen oilseeds. Essentially, our studies suggest that potential exists to enhance castor oil yield through increasing exposure of the inflorescences to sunlight either by genetically changing the plant architecture (smart canopy) or its growing environment. PMID:26589321

  19. Architecture humanitarian emergencies

    DEFF Research Database (Denmark)

    Gomez-Guillamon, Maria; Eskemose Andersen, Jørgen; Contreras, Jorge Lobos;

    2013-01-01

    Introduced by scientific articles conserning architecture and human rights in light of cultures, emergencies, social equality and sustainability, democracy, economy, artistic development and science into architecture. Concluding in definition of needs for new roles, processes and education of...... architecture. Followed by articles focusing on interdisciplinary research and design of emergency shelters as well as educational environments. Finally concretized in 35 studies from international workshops arranged globally on and by different architect schools: Royal Danish Academy of Fine Arts in Denmark...

  20. Seed quality in informal seed systems

    OpenAIRE

    Biemond, P.C.

    2013-01-01

    Keywords:     informal seed systems, seed recycling, seed quality, germination, seed pathology, seed health, seed-borne diseases, mycotoxigenic fungi, Fusarium verticillioides, mycotoxins, Vigna unguiculata, Zea mays, Nigeria.   Seed is a crucial input for agricultural production. Approximately 80% of the smallholder farmers in Africa depend for their seed on the informal seed system, consisting of farmers involved in selection, production and dissemination of seed. The la...

  1. Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.

    Science.gov (United States)

    Tabe, Linda; Wirtz, Markus; Molvig, Lisa; Droux, Michel; Hell, Ruediger

    2010-03-01

    There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosynthesis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F(2) seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein. PMID:19939888

  2. Architectural Contestation

    OpenAIRE

    Merle, J.

    2012-01-01

    This dissertation addresses the reductive reading of Georges Bataille's work done within the field of architectural criticism and theory which tends to set aside the fundamental ‘broken’ totality of Bataille's oeuvre and also to narrowly interpret it as a mere critique of architectural form, consequently presenting it either as the negation of all form of architecture or as the critique of 'classical' architectural forms. Against this ‘appropriation’, i.e. this reductive reading and the subse...

  3. Architectural Theatricality

    DEFF Research Database (Denmark)

    Tvedebrink, Tenna Doktor Olsen; Fisker, Anna Marie; Kirkegaard, Poul Henning

    2013-01-01

    and recovery through the architecture framing eating experiences, this article examines, from a theoretical perspective, two less debated concepts relating to hospitality called food design and architectural theatricality. In architectural theory the nineteenth century German architect Gottfried...... Semper is known for his writings on theatricality, understood as a holistic design approach emphasizing the contextual, cultural, ritual and social meanings rooted in architecture. Relative hereto, the International Food Design Society recently argued, in a similar holistic manner, that the methodology...

  4. VLSI architecture

    Energy Technology Data Exchange (ETDEWEB)

    Randell, B.; Treleaven, P.C.

    1983-01-01

    This book is a collection of course papers which discusses the latest (1982) milestone of electronic building blocks and its effect on computer architecture. Contributions range from selecting a VLSI process technology to Japan's Fifth Generation Computer Architecture. Contents, abridged: VLSI and machine architecture. Graphic design aids: HED and FATFREDDY. On the LUCIFER system. Clocking of VLSI circuits. Decentralised computer architectures for VLSI. Index.

  5. On the role played by radiotoxins in stimulation of the growth and development of irradiated seeds

    International Nuclear Information System (INIS)

    Potato tubers were used for the experiment. A part of them was irradiated with 10 kR of 60Co γ-rays. After 24h of storage in the dark, the tubers were cooled for 2h and homogenized. The radiotoxin, the so-called substance X could be isolated only from irradiated tubers. The chemical properties of the substance X are described. Seeds of radish, castor-bean and barley were soaked for 24h in the radiotoxin solution, grown under standard conditions and scored for the length of the main root and the height of the sprout on the 5th day of germination. At concentrations of the order of 10-3 M substance X inhibits the germination and growth of the seedlings. Concentrations of 1 x 10-4 to 1 x 10-6 M proved to have a marked stimulating effect. The mechanism of this stimulation is discussed. (MG)

  6. Architecture of Network Systems

    CERN Document Server

    Serpanos, Dimitrios

    2011-01-01

    Network systems require technical skills in computer architecture, design methodologies, algorithm design, and networking. Architecture of Network Systems explains the practice and methodologies that will allow you to solve a broad range of problems in system design, including problems related to security, quality of service, performance, manageability, and more. Leading researchers Dimitrios Serpanos and Tilman Wolf develop architectures for all network sub-systems, bridging the gap between operation and VLSI. Discussing the major challenges in the design of networks and the architectures tha

  7. IT Architecture For Dummies

    CERN Document Server

    Hausman, Kalani Kirk

    2010-01-01

    A solid introduction to the practices, plans, and skills required for developing a smart system architecture. Information architecture combines IT skills with business skills in order to align the IT structure of an organization with the mission, goals, and objectives of its business. This friendly introduction to IT architecture walks you through the myriad issues and complex decisions that many organizations face when setting up IT systems to work in sync with business procedures. Veteran IT professional and author Kirk Hausman explains the business value behind IT architecture and provides

  8. Information architecture. Volume 3: Guidance

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-04-01

    The purpose of this document, as presented in Volume 1, The Foundations, is to assist the Department of Energy (DOE) in developing and promulgating information architecture guidance. This guidance is aimed at increasing the development of information architecture as a Departmentwide management best practice. This document describes departmental information architecture principles and minimum design characteristics for systems and infrastructures within the DOE Information Architecture Conceptual Model, and establishes a Departmentwide standards-based architecture program. The publication of this document fulfills the commitment to address guiding principles, promote standard architectural practices, and provide technical guidance. This document guides the transition from the baseline or defacto Departmental architecture through approved information management program plans and budgets to the future vision architecture. This document also represents another major step toward establishing a well-organized, logical foundation for the DOE information architecture.

  9. New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination.

    Science.gov (United States)

    Ruiz-Ballesta, Isabel; Baena, Guillermo; Gandullo, Jacinto; Wang, Liqun; She, Yi-Min; Plaxton, William Charles; Echevarría, Cristina

    2016-05-01

    Phosphoenolpyruvate carboxylase (PEPC; E.C. 4.1.1.31) was characterized in developing and germinating sorghum seeds, focusing on the transcript and polypeptide abundance of multiple plant-type phosphoenolpyruvate carboxylase (PTPC) genes, and the post-translational modification of each isoenzyme by phosphorylation versus monoubiquitination during germination. We observed high levels of SbPPC4 (Sb07g014960) transcripts during early development (stage I), and extensive transcript abundance of SbPPC2 (Sb02g021090) and SbPPC3 (Sb04g008720) throughout the entire life cycle of the seed. Although tandem mass spectrometry (MS) analysis of immunopurified PTPC indicated that four different PTPC isoenzymes were expressed in the developing and germinating seeds, SbPPC3 was the most abundant isozyme of the developing seed, and of the embryo and the aleurone layer of germinating seeds. In vivo phosphorylation of the different PTPC isoenzymes at their conserved N-terminal seryl phosphorylation site during germination was also established by MS/MS analysis. Furthermore, three of the four isoenzymes were partially monoubiquitinated, with MS/MS pinpointing SbPPC2 and SbPPC3 monoubiquitination at the conserved Lys-630 and Lys-624 residues, respectively. Our results demonstrate that monoubiquitination and phosphorylation simultaneously occur in vivo with different PTPC isozymes during seed germination. In addition, we show that PTPC monoubiquitination in germinating sorghum seeds always increases at stage II (emergence of the radicle), is maintained during the aerobic period of rapid cell division and reserve mobilization, and remains relatively constant until stage IV-V when coleoptiles initiate the formation of the photosynthetic tissues. PMID:27194739

  10. Design Process in Landscape Architecture: Developing a Learning Guide for the Design Workshop Archives at Utah State University

    OpenAIRE

    Gottfredson, John A.

    2014-01-01

    In 2011, Utah State University created the Design Workshop™ Landscape Architecture Archive [Archive] housed in the Merrill-Cazier Library’s Special Collections and Archives. These archives constitute a valuable and unique learning opportunity for students, researchers and professionals in landscape architecture. The Archive consists of the process documents and work product of over 40 years of professional practice in landscape architecture by Design Workshop™, a leading landscape architectur...

  11. Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.

    Science.gov (United States)

    da Silva, Danielle Costenaro; da Silveira Falavigna, Vítor; Fasoli, Marianna; Buffon, Vanessa; Porto, Diogo Denardi; Pappas, Georgios Joannis; Pezzotti, Mario; Pasquali, Giancarlo; Revers, Luís Fernando

    2016-01-01

    The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. PMID:27610237

  12. Architectural Theatricality

    DEFF Research Database (Denmark)

    Tvedebrink, Tenna Doktor Olsen

    This PhD thesis is motived by a personal interest in the theoretical, practical and creative qualities of architecture. But also a wonder and curiosity about the cultural and social relations architecture represents through its occupation with both the sciences and the arts. Inspired by present...... initiatives in Aalborg Hospital to overcome patient undernutrition by refurbishing eating environments, this thesis engages in an investigation of the interior architectural qualities of patient eating environments. The relevance for this holistic perspective, synthesizing health, food and architecture, is...... the current building of a series of Danish ‘super hospitals’ and an increased focus among architectural practices on research-based knowledge produced with the architectural sub-disciplines Healing Architecture and Evidence-Based Design. The problem is that this research does not focus on patient...

  13. The BURP domain protein AtUSPL1 of Arabidopsis thaliana is destined to the protein storage vacuoles and overexpression of the cognate gene distorts seed development.

    Science.gov (United States)

    Van Son, Le; Tiedemann, Jens; Rutten, Twan; Hillmer, Stefan; Hinz, Giselbert; Zank, Thorsten; Manteuffel, Renate; Bäumlein, Helmut

    2009-11-01

    BURP domain proteins comprise a broadly distributed, plant-specific family of functionally poorly understood proteins. VfUSP (Vicia faba Unknown Seed Protein) is the founding member of this family. The BURP proteins are characterized by a highly conserved C-terminal protein domain with a characteristic cysteine-histidine pattern. The Arabidopsis genome contains five BURP-domain encoding genes. Three of them are similar to the non-catalytic beta-subunit of the polygalacturonase of tomato and form a distinct subgroup. The remaining two genes are AtRD22 and AtUSPL1. The deduced product of AtUSPL1 is similar in size and sequence to VfUSP and that of the Brassica napus BNM2 gene which is expressed during microspore-derived embryogenesis. The protein products of BURP genes have not been found, especially that of VfUSP despite a great deal of interest arising from copious transcription of the gene in seeds. Here, we demonstrate that VfUSP and AtUSPL1 occur in cellular compartments essential for seed protein synthesis and storage, like the Golgi cisternae, dense vesicles, prevaculoar vesicles and the protein storage vacuoles in the parenchyma cells of cotyledons. Ectopic expression of AtUSPL1 leads to a shrunken seed phenotype; these seeds show structural alterations in their protein storage vacuoles and lipid vesicles. Furthermore, there is a reduction in the storage protein content and a perturbation in the seed fatty acid composition. However, loss of AtUSP1 gene function due to T-DNA insertions does not lead to a phenotypic change under laboratory conditions even though the seeds have less storage proteins. Thus, USP is pertinent to seed development but its role is likely shared by other proteins that function well enough under the laboratory growth conditions. PMID:19639386

  14. Developing Enterprise Architectures to Address the Enterprise Dilemma of Deciding What Should Be Sustained versus What Should Be Changed

    Science.gov (United States)

    Harrell, J. Michael

    2011-01-01

    Enterprise architecture is a relatively new concept that arose in the latter half of the twentieth century as a means of managing the information technology resources within the enterprise. Borrowing from the disciplines of brick and mortar architecture, software engineering, software architecture, and systems engineering, the enterprise…

  15. Space station data system analysis/architecture study. Task 2: Options development, DR-5. Volume 2: Design options

    Science.gov (United States)

    1985-01-01

    The primary objective of Task 2 is the development of an information base that will support the conduct of trade studies and provide sufficient data to make key design/programmatic decisions. This includes: (1) the establishment of option categories that are most likely to influence Space Station Data System (SSDS) definition; (2) the identification of preferred options in each category; and (3) the characterization of these options with respect to performance attributes, constraints, cost and risk. This volume contains the options development for the design category. This category comprises alternative structures, configurations and techniques that can be used to develop designs that are responsive to the SSDS requirements. The specific areas discussed are software, including data base management and distributed operating systems; system architecture, including fault tolerance and system growth/automation/autonomy and system interfaces; time management; and system security/privacy. Also discussed are space communications and local area networking.

  16. A Communication Architecture For IVHS

    OpenAIRE

    Streisand, Susan L.; Walrand, Jean

    1992-01-01

    This paper documents the development of a communications architecture for Intelligent Vehicle Highway Systems (IVHS) being studied by the California PATH Program. The authors use a layered architecture based on the OSI 7- layer reference model.

  17. Branch architecture, light interception and crown development in saplings of a plagiotropically branching tropical tree, Polyalthia jenkinsii (Annonaceae).

    Science.gov (United States)

    Osada, Noriyuki; Takeda, Hiroshi

    2003-01-01

    To investigate crown development patterns, branch architecture, branch-level light interception, and leaf and branch dynamics were studied in saplings of a plagiotropically branching tree species, Polyalthia jenkinsii Hk. f. & Thoms. (Annonaceae) in a Malaysian rain forest. Lengths of branches and parts of the branches lacking leaves ('bare' branches) were smaller in upper branches than in lower branches within crowns, whereas lengths of 'leafy' parts and the number of leaves per branch were larger in intermediate than in upper and lower branches. Maximum diffuse light absorption (DLA) of individual leaves was not related to sapling height or branch position within crowns, whereas minimum DLA was lower in tall saplings. Accordingly, branch-level light interception was higher in intermediate than in upper and lower branches. The leaf production rate was higher and leaf loss rate was smaller in upper than in intermediate and lower branches. Moreover, the branch production rate of new first-order branches was larger in the upper crowns. Thus, leaf and branch dynamics do not correspond to branch-level light interception in the different canopy zones. As a result of architectural constraints, branches at different vertical positions experience predictable light microenvironments in plagiotropic species. Accordingly, this pattern of carbon allocation among branches might be particularly important for growth and crown development in plagiotropic species. PMID:12495920

  18. Clinical document architecture.

    Science.gov (United States)

    Heitmann, Kai

    2003-01-01

    The Clinical Document Architecture (CDA), a standard developed by the Health Level Seven organisation (HL7), is an ANSI approved document architecture for exchange of clinical information using XML. A CDA document is comprised of a header with associated vocabularies and a body containing the structural clinical information. PMID:15061557

  19. Workflow automation architecture standard

    Energy Technology Data Exchange (ETDEWEB)

    Moshofsky, R.P.; Rohen, W.T. [Boeing Computer Services Co., Richland, WA (United States)

    1994-11-14

    This document presents an architectural standard for application of workflow automation technology. The standard includes a functional architecture, process for developing an automated workflow system for a work group, functional and collateral specifications for workflow automation, and results of a proof of concept prototype.

  20. Brief Analysis Economy Sustainable Development Concept to World Expo Architectural Design Influence

    Institute of Scientific and Technical Information of China (English)

    杨秀娟

    2011-01-01

    The economical sustainable development is a kind of reasonable economic development shape,it is regarding Chinese each economic development guidance function of taking the characteristic economy——convention economy development which rising rapidly to have

  1. Map-Based Cloning of Seed Dormancy1-2 Identified a Gibberellin Synthesis Gene Regulating the Development of Endosperm-Imposed Dormancy in Rice.

    Science.gov (United States)

    Ye, Heng; Feng, Jiuhuan; Zhang, Lihua; Zhang, Jinfeng; Mispan, Muhamad S; Cao, Zhuanqin; Beighley, Donn H; Yang, Jianchang; Gu, Xing-You

    2015-11-01

    Natural variation in seed dormancy is controlled by multiple genes mapped as quantitative trait loci in major crop or model plants. This research aimed to clone and characterize the Seed Dormancy1-2 (qSD1-2) locus associated with endosperm-imposed dormancy and plant height in rice (Oryza sativa). qSD1-2 was delimited to a 20-kb region, which contains OsGA20ox2 and had an additive effect on germination. Naturally occurring or induced loss-of-function mutations of the gibberellin (GA) synthesis gene enhanced seed dormancy and also reduced plant height. Expression of this gene in seeds (including endospermic cells) during early development increased GA accumulation to promote tissue morphogenesis and maturation programs. The mutant allele prevalent in semidwarf cultivars reduced the seed GA content by up to 2-fold at the early stage, which decelerated tissue morphogenesis including endosperm cell differentiation, delayed abscisic acid accumulation by a shift in the temporal distribution pattern, and postponed dehydration, physiological maturity, and germinability development. As the endosperm of developing seeds dominates the moisture equilibrium and desiccation status of the embryo in cereal crops, qSD1-2 is proposed to control primary dormancy by a GA-regulated dehydration mechanism. Allelic distribution of OsGA20ox2, the rice Green Revolution gene, was associated with the indica and japonica subspeciation. However, this research provided no evidence that the primitive indica- and common japonica-specific alleles at the presumably domestication-related locus functionally differentiate in plant height and seed dormancy. Thus, the evolutionary mechanism of this agriculturally important gene remains open for discussion. PMID:26373662

  2. Seed Priming Influence on Early Crop Growth, Phenological Development and Yield Performance of Linola (Linum usitatissimum L.)

    Institute of Scientific and Technical Information of China (English)

    Hafeez ur Rehman; Muhammad Qaiser Nawaz; Shahzad Maqsood Ahmed Basra; Irfan Afzal; Azra Yasmeen; Fayyaz ul-Hassan

    2014-01-01

    Reduced early crop growth and limited branching are amongst yield limiting factors of linola. Field response of seed priming treatments viz. 50 mmol L-1 salicylic acid (SA), 2.2%CaCl2 and 3.3%moringa leaf extract (MLE) including untreated dry and hydropriming controls was evaluated on early crop growth and yield performance of linola. Osmopriming with CaCl2 reduced emergence time and produced the highest seedling fresh and dry weights including Chl. a contents. Osmopriming with CaCl2 reduced crop branching and lfowering and maturity times and had the maximum plant height, number of branches, tillers, pods and seeds per pod followed by MLE. Increase in seed weight, biological and seed yields was 9.30, 34.16 and 39.49%, harvest index (4.12%) and oil contents (13.39%) for CaCl2 osmopriming. Positive relationship between emergence and seedling vigor traits, 100-seed weight, seed yield with maturity time, 100-seed weight and seed yield were found. The study concludes that seed osmopriming with CaCl2 or MLE can play signiifcant role to improve early crop growth and seed yields of linola.

  3. Economics-driven software architecture

    CERN Document Server

    Mistrik, Ivan; Kazman, Rick; Zhang, Yuanyuan

    2014-01-01

    Economics-driven Software Architecture presents a guide for engineers and architects who need to understand the economic impact of architecture design decisions: the long term and strategic viability, cost-effectiveness, and sustainability of applications and systems. Economics-driven software development can increase quality, productivity, and profitability, but comprehensive knowledge is needed to understand the architectural challenges involved in dealing with the development of large, architecturally challenging systems in an economic way. This book covers how to apply economic consider

  4. Study and development of an Iridium-192 seed for use in ophthalmic cancer; Estudo e desenvolvimento de uma semente de iridio-192 para aplicacao em cancer oftalmico

    Energy Technology Data Exchange (ETDEWEB)

    Mattos, Fabio Rodrigues de

    2013-07-01

    Even ocular tumors are not among the cases with a higher incidence, they affect the population, especially children. The Institute of Energy and Nuclear Research (IPEN-CNEN/SP) in partnership with Escola Paulista de Medicina (UNIFESP), created a project to develop and implement a alternative treatment for ophthalmic cancer that use brachytherapy iridium-192 seeds. The project arose by reason of the Escola Paulista treat many cancer cases within the Unified Health System (SUS) and the research experience of sealed radioactive sources group at IPEN. The methodology was developed from the available infrastructure and the experience of researchers. The prototype seed presents with a core (192-iridium alloy of iridium-platinum) of 3.0 mm long sealed by a capsule of titanium of 0.8 mm outside diameter, 0.05 mm wall thickness and 4,5mm long. This work aims to study and develop a seed of iridium-192 from a platinum-iridium alloy. No study on the fabrication of these seeds was found in available literature. It was created a methodology that involved: characterization of the material used in the core, creation of device for neutron activation irradiation and and seed sealing tests. As a result, proved the feasibility of the method. As a suggestion for future work, studies regarding metrology and dosimetry of these sources and improvement of the methodology should be carried out, for future implementation in national scope. (author)

  5. Architectural freedom and industrialised architecture

    DEFF Research Database (Denmark)

    Vestergaard, Inge

    2012-01-01

    Architectural freedom and industrialized architecture. Inge Vestergaard, Associate Professor, Cand. Arch. Aarhus School of Architecture, Denmark Noerreport 20, 8000 Aarhus C Telephone +45 89 36 0000 E-mai l inge.vestergaard@aarch.dk Based on the repetitive architecture from the "building boom" 1960...... to 1973 it is discussed how architects can handle these Danish element and montage buildings through the transformation to upgraded aesthetical, functional and energy efficient architecture. The method used is analysis of cases, parallels to literature studies and producer interviews. This analysis...... compares "best practice" in Denmark and "best practice" in Austria. The modern architects accepted the fact that industrialized architecture told the storey of repetition and monotonous as basic condition. This article aims to explain that architecture can be thought as a complex and diverse design through...

  6. Axon Initial Segment Cytoskeleton: Architecture, Development, and Role in Neuron Polarity.

    Science.gov (United States)

    Jones, Steven L; Svitkina, Tatyana M

    2016-01-01

    The axon initial segment (AIS) is a specialized structure in neurons that resides in between axonal and somatodendritic domains. The localization of the AIS in neurons is ideal for its two major functions: it serves as the site of action potential firing and helps to maintain neuron polarity. It has become increasingly clear that the AIS cytoskeleton is fundamental to AIS functions. In this review, we discuss current understanding of the AIS cytoskeleton with particular interest in its unique architecture and role in maintenance of neuron polarity. The AIS cytoskeleton is divided into two parts, submembrane and cytoplasmic, based on localization, function, and molecular composition. Recent studies using electron and subdiffraction fluorescence microscopy indicate that submembrane cytoskeletal components (ankyrin G, βIV-spectrin, and actin filaments) form a sophisticated network in the AIS that is conceptually similar to the polygonal/triangular network of erythrocytes, with some important differences. Components of the AIS cytoplasmic cytoskeleton (microtubules, actin filaments, and neurofilaments) reside deeper within the AIS shaft and display structural features distinct from other neuronal domains. We discuss how the AIS submembrane and cytoplasmic cytoskeletons contribute to different aspects of AIS polarity function and highlight recent advances in understanding their AIS cytoskeletal assembly and stability. PMID:27493806

  7. Axon Initial Segment Cytoskeleton: Architecture, Development, and Role in Neuron Polarity

    Science.gov (United States)

    Svitkina, Tatyana M.

    2016-01-01

    The axon initial segment (AIS) is a specialized structure in neurons that resides in between axonal and somatodendritic domains. The localization of the AIS in neurons is ideal for its two major functions: it serves as the site of action potential firing and helps to maintain neuron polarity. It has become increasingly clear that the AIS cytoskeleton is fundamental to AIS functions. In this review, we discuss current understanding of the AIS cytoskeleton with particular interest in its unique architecture and role in maintenance of neuron polarity. The AIS cytoskeleton is divided into two parts, submembrane and cytoplasmic, based on localization, function, and molecular composition. Recent studies using electron and subdiffraction fluorescence microscopy indicate that submembrane cytoskeletal components (ankyrin G, βIV-spectrin, and actin filaments) form a sophisticated network in the AIS that is conceptually similar to the polygonal/triangular network of erythrocytes, with some important differences. Components of the AIS cytoplasmic cytoskeleton (microtubules, actin filaments, and neurofilaments) reside deeper within the AIS shaft and display structural features distinct from other neuronal domains. We discuss how the AIS submembrane and cytoplasmic cytoskeletons contribute to different aspects of AIS polarity function and highlight recent advances in understanding their AIS cytoskeletal assembly and stability. PMID:27493806

  8. Muskmelon seed germination and seedling development in response to seed priming Germinação de sementes e desenvolvimento de plântulas de melão em resposta ao condicionamento osmótico

    Directory of Open Access Journals (Sweden)

    Warley Marcos Nascimento

    2003-02-01

    Full Text Available Important factors affecting seed priming have not been extensively reported in muskmelon (Cucumis melo L. studies. The optimization of the seed priming technique becomes very important at the commercial scale. Little information has been reported on seedling development of muskmelon subsequent to seed priming. Seeds of muskmelon were primed in darkness at 25°C in different solutions and three osmotic potentials. Seeds were also primed with and without aeration during different periods. In relation to osmotic solutions, an osmotic potential around -1.30 MPa is most adequate for muskmelon priming. Salt solutions gave better germination rate but were deleterious for seed germination, especially at higher osmotic potentials. Aeration of the soaking salt solution gave faster germination at 17°C, and because of the early germination, these treatments probably presented a better seedling development. Deleterious effect on total seed germination was observed for long soaking periods with aeration. Fungal growth increased on seeds primed in aerated solutions. Seeds from priming treatments had a better germination rate and seedling development under 17 and 25°C.Importantes fatores afetando o condicionamento osmótico não têm sido extensivamente relatados em sementes de melão (Cucumis melo L.. A otimização do condicionamento osmótico é extremamente importante em uma escala comercial. Poucas informações têm sido relatadas quanto ao desenvolvimento de plântulas em resposta ao condicionamento osmótico de sementes de melão. Sementes de melão foram condicionadas a 25°C, na ausência de luz, em diferentes soluções osmóticas utilizando três potenciais osmóticos para cada solução. Em outro estudo, sementes foram condicionadas com ou sem aeração durante vários períodos. Indiferentemente das soluções osmóticas, o potencial osmótico em torno de - 1,30 MPa foi mais adequado para o condicionamento osmótico. Sementes condicionadas em

  9. A system for generating virtual seeds

    Directory of Open Access Journals (Sweden)

    Sako Y.

    1998-01-01

    Full Text Available Seed analysts need to identify seeds, and seed catalogs are used as a reference to accomplish this task. Conventional seed catalogs supply two-dimensional photographs and hand-drawn diagrams. In this study, a new, three-dimensional representation of seeds is developed to supplement these traditional photographs and drawings. QuickTime VR is a promising method for viewing three-dimensional objects on a computer screen. It permits manipulation of an object by rotating and viewing it from any pre-specified angle at an interactive speed, allowing the viewer the sense of examining a hand-held object. In this study, QuickTime VR object movies of seeds were created as interactive "movies" of seeds that can be rotated and scaled to give the viewer the sensation of examining actual seeds. This approach allows the examination of virtual seeds from any angle, permitting more accurate identification of seeds by seed analysts.

  10. Architecture, development and implementation of a SWIR to visible integrated up-conversion imaging device

    Science.gov (United States)

    Sarusi, Gabby; Templeman, Tzvi; Hechster, Elad; Nissim, Nimrod; Vitenberg, Vladimir; Maman, Nitzan; Tal, Amir; Solodar, Assi; Makov, Guy; Abdulhalim, Ibrahim; Visoly-Fisher, Iris; Golan, Yuval

    2016-04-01

    A new concept of short wavelength infrared (SWIR) to visible upconversion integrated imaging device is proposed, modeled and some initial measured results are presented. The device is a hybrid inorganic-organic device that comprises six nano-metric scale sub-layers grown on n-type GaAs substrates. The first layer is a ~300nm thick PbSe nano-columnar absorber layer grown in (111) orientation to the substrate plan (100), with a diameter of 8- 10nm and therefore exhibit quantum confinement effects parallel to the substrate and bulk properties perpendicular to it. The advantage of this structure is the high oscillator strength and hence absorption to incoming SWIR photons while maintaining the high bulk mobility of photo-excited charges along the columns. The top of the PbSe absorber layer is coated with 20nm thick metal layer that serves as a dual sided mirror, as well as a potentially surface plasmon enhanced absorption in the PbSe nano-columns layer. The photo-excited charges (holes and electrons in opposite directions) are drifted under an external applied field to the OLED section (that is composed of a hole transport layer, an emission layer and an electron transport layer) where they recombine with injected electron from the transparent cathode and emit visible light through this cathode. Due to the high absorption and enhanced transport properties this architecture has the potential of high quantum efficiency, low cost and easy implementation in any optical system. As a bench-mark, alternative concept where InGaAs/InP heterojunction couple to liquid crystal optical spatial light modulator (OSLM) structure was built that shows a full upconversion to visible of 1550nm laser light.

  11. A tutorial for sandstone petrology: architecture and development of an interactive program for teaching highly visual material

    Science.gov (United States)

    Choh, Suk-Joo; Milliken, Kitty L.; McBride, Earle F.

    2003-11-01

    We have developed an interactive computer-based tutorial in sandstone petrology for undergraduate-level students. The goal of this tutorial is to provide students exposure to the highly visual subject matter of petrography outside the confines of organized laboratory exercises. This paper describes the architecture and development procedures of the current version of the sandstone petrography tutorial, and offers a possible model for similar development approaches in other fields of petrography or in any other field that utilizes large quantities of visual material such as remote sensing image interpretation or seismic interpretation. The tutorial is an interactive photomicrograph archive with sufficient content and flexible architecture that functions as a virtual laboratory instructor as well as a stand-alone reference. The current tutorial was programmed using Macromedia Authorware v.6.0 and supports both Windows-based and MacOS personal computers. The tutorial is constructed around the Folk sandstone classification scheme (quartzarenite, arkose, and litharenite), and an additional section addresses grains other than quartz, feldspar, and lithic fragments and sandstones dominated by these grains. The user interface is designed to take minimal portion of the screen area so that the screen can closely mimic the type of view seen by a student peering down a microscope. Each photomicrograph in the tutorial is basically unadorned until the user actively calls up information that is temporarily displayed over the image, inducing the user to search for information and actively "ask" to be informed with a mouse click. The structure of the tutorial permits multiple strategies of program use, as a linear tutorial, tutorial driven by thumbnail browser, and as a searchable reference.

  12. “Pushing the Envelope” a modeling-based approach to the development of organic, responsive architectural form

    Directory of Open Access Journals (Sweden)

    David Yearley

    2012-11-01

    Full Text Available This paper tests design procedures for the development of complex, organic architectural forms. It illustrates a postgraduate student design process, implementing a development sequence based on the intelligent manipulation of architectural envelopes using a variety of existing modeling tools and emerging digital techniques. These stages of development respond to imposed spatial and environmental constraints. The tests began with full-scale modeling of small segments. The major constraints at this stage were spatial requirements and the physical characteristics of materials. The forms derived from the bending properties of prestressed green timber and the dimensions of shingle cladding. This was followed by digital 3D modeling using common commercial applications. At this stage initial models were derived from a traditional space requirement brief. The envelopes for these activities were then manipulated to respond to the spatial limitations imposed by surrounding buildings. This digital modeling process metaphorically “pushed the limits” as vertices of the envelope model were stretched and shifted to achieve a perceived “fit” between the two sets of spatial dimensions. The spatially manipulated geometry was then imported into Ecotect, an environmental analysis package. As an example, the envelope’s morphology and cladding material options on the acoustic qualities of the surrounding space were tested. The improved geometry was then imported into a Virtual Reality room, in which the spatial experience was simulated in presentations to the design team and potential occupants. This room utilized six projectors to create an immersive experience to users wearing stereoscopic goggles, and moving in a space surrounded by three large screens, creating a CAVE-like presentation space. Finally there was an attempt to complete the circle by returning from the simulated world to the physical worlds, by creating full-scale models from the digital

  13. Glioblastoma with spinal seeding

    Energy Technology Data Exchange (ETDEWEB)

    Fakhrai, N.; Fazeny-Doerner, B.; Marosi, C. [Clinical Div. of Oncology, Dept. of Medicine I, Univ. of Vienna (Austria); Czech, T. [Dept. of Neurosurgery, Univ. of Vienna (Austria); Diekmann, K. [Dept. of Radiooncology, Univ. of Vienna (Austria); Birner, P.; Hainfellner, J.A. [Clinical Inst. for Neurology, Univ. of Vienna (Austria); Prayer, D. [Dept. of Neuroradiology, Univ. of Vienna (Austria)

    2004-07-01

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  14. Physalis peruviana seed storage

    Directory of Open Access Journals (Sweden)

    Cíntia L. M. de Souza

    2016-03-01

    Full Text Available ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C. Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture content varied during the storage period, but was always higher for seeds kept at -196 ºC. These seeds kept high germination percentage in water until 16 months, regardless of the tested temperature; however, in salt solution the germination percentage was significantly reduced.

  15. Seed recovery and regeneration

    International Nuclear Information System (INIS)

    Magnetohydrodynamic's (MHD) electric power generation capability depends on Faraday's law of induction. An ionized gas, called plasma, passes through a fixed magnetic field. The plasma is produced by seeding flue gases at a temperature of around 2500 deg C with potassium carbonate or formate. In a coal-fired open cycle MHD power plant the potassium seed material is in direct contact with the polluted flue gas from coal combustion. The molten slag droplets will capture a certain amount of potassium. The sulphur dioxide in the flue gases will react with potassium to potassium sulphate. The spent seed material has to be recovered and reprocessed so that the potassium can be reused as seed material. We can distinguish a preprocessing and a regeneration step. Seed preprocessing removes the minerals and volatile coal constituents from the potassium salts. Seed regeneration transforms the purified potassium sulphate into potassium carbonate or formate. Eight regeneration processes have been evaluated. The processes that convert the sulphur of the coal into saleable sulphur or sulphuric acid are still at an early stage of development. Considering all pros and cons, the Formate/Econoseed process appears most suited for seed regeneration purposes in coal-fired MHD systems. For an advanced MHD power plant the mass flow rates of the flue gas and of the potassium containing off-product streams will be calculated. It will be shown that the sulphur content of coal will influence the economical efficiency of the power plant

  16. Glioblastoma with spinal seeding

    International Nuclear Information System (INIS)

    Background: extracranial seeding of glioblastoma multiforme (GBM) is very rare and its development depends on several factors. This case report describes two patients suffering from GBM with spinal seeding. In both cases, the anatomic localization of the primary tumor close to the cerebrospinal fluid (CSF) was the main factor for spinal seeding. Case reports: two patients with GBM and spinal seeding are presented. After diagnosis of spinal seeding, both patients were highly symptomatic from their spinal lesions. Case 1 experienced severe pain requiring opiates, and case 2 had paresis of lower limbs as well as urinary retention/incontinence. Both patients were treated with spinal radiation therapy. Nevertheless, they died 3 months after diagnosis of spinal seeding. Results: in both patients the diagnosis of spinal seeding was made at the time of cranial recurrence. Both tumors showed close contact to the CSF initially. Even though the patients underwent intensive treatment, it was not possible to keep them in a symptom-free state. Conclusion: because of short survival periods, patients deserve optimal pain management and dedicated palliative care. (orig.)

  17. Meet French Architecture in China

    Institute of Scientific and Technical Information of China (English)

    Susan

    2005-01-01

    On April 5, a French architectural exhibition entitled ""French Visions: City, Architecture and Sustainable Development"" was unveiled in Beijing Urban Planning Center. As a key exhibition within the framework of French Year in China, the exhibition will also be displayed in Shanghai and Chongqing. The largest exhibition of its kind ever held in China, French Visions extensively showed accomplishments of contemporary French architectural design.

  18. A Development Architecture for Serious Games Using BCI (Brain Computer Interface) Sensors

    OpenAIRE

    Kyhyun Um; Yunsick Sung; Kyungeun Cho

    2012-01-01

    Games that use brainwaves via brain–computer interface (BCI) devices, to improve brain functions are known as BCI serious games. Due to the difficulty of developing BCI serious games, various BCI engines and authoring tools are required, and these reduce the development time and cost. However, it is desirable to reduce the amount of technical knowledge of brain functions and BCI devices needed by game developers. Moreover, a systematic BCI serious game development process is required. In this...

  19. Beyond product architecture: Division of labour and competence accumulation in complex product development

    OpenAIRE

    Markus C. Becker; Francesco Zirpoli

    2011-01-01

    This paper considers the trade-off between leveraging external sources of innovation by outsourcing design and engineering activities and the ability to develop internal product development competences. The trade-off arises because the division of labor within and across firms' boundaries has a crucial role in shaping competence development processes, especially because the division of labor also influences opportunities for learning by doing. In new product development projects, learning by ...

  20. Collaborative product development : a groupware system based on SOA [Service Oriented Architecture

    OpenAIRE

    Melo, Daniel do N.; Varela, Maria Leonilde Rocha; Silva, Sílvio Carmo

    2010-01-01

    Product Development (PD) can be enhanced through web-based collaboration of working teams. This collaboration enables professionals to work simultaneously on a same project, e.g. the development of a new product. Collaborative PD requires networking resources, and applications needed by collaborating teams in the design and management of product development projects. Using this collaborative approach to PD can bring benefits on cost and speed of development, and also on ideas, all important f...