WorldWideScience

Sample records for archaeo-eukaryotic gins proteins

  1. SOURCE ASSESSMENT: COTTON GINS

    Science.gov (United States)

    This report describes a study of air pollutants from cotton gins. Cotton gins separate cotton fibers from cottonseed and trash. During the 1976 crop year, 2.6 x 10 to the 6th power metric tons of lint cotton were ginned. Particulates composed of cotton dust, cotton lint, fine-lea...

  2. Evaluation of cotton genotypes for ginning energy and ginning rate

    Science.gov (United States)

    Reducing ginning energy use through cultivar improvement could reduce ginning and energy cost. The objective of this study was to detect genetic variability for ginning energy and ginning rate. Thirty four conventional and twelve transgenic genotypes were evaluated in 2008 and 2009 for ginning energ...

  3. Energy from cotton gin waste

    Energy Technology Data Exchange (ETDEWEB)

    Goodin, P.L.

    1977-04-01

    The US Cotton Ginning Research Laboratory installed a small, multi-chamber incinerator to study its ability to convert cotton gin waste into useable on-site energy. The incinerator operates on a controlled or starved air principle with a maximum capacity of 350-400 lbs of trash/hr. Tests indicate that 31.4% of the heat from combustion of the gin trash can be recovered and efficiently delivered to the seed-cotton drying system.

  4. Predicting Fiber Quality After Commercial Ginning Based on Fiber Obtained with Laboratory-Scale Gin Stands

    Science.gov (United States)

    It is often useful, especially in research, to measure properties of cotton lint ginned from small seed cotton samples with small laboratory scale gin stands and use the results to estimate properties of lint after commercial ginning, but these gin stands differ from commercial gins which brings int...

  5. A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS

    Institute of Scientific and Technical Information of China (English)

    Reiko Hayashi; Takako Arauchi; Moe Tategu; Yuya Goto; Kenichi Yoshida

    2006-01-01

    GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step of DNA replication. Although the yeast and Xenopus GINS genes are well documented, their orthologous genes in higher eukaryotes are not fully characterized.In this study, we report the genomic structure and transcriptional regulation of mammalian GINS genes. Serum stimulation increased the GINS Mrna levels in human cells. Reporter gene assay using putative GINS promoter sequences revealed that the expression of mammalian GINS is regulated by 17β-Estradiolstimulated estrogen receptor α, and human PSF3 acts as a gene responsive to transcription factor E2F1. The goal of this study is to present the current data so as to encourage further work in the field of GINS gene regulation and functions in mammalian cells.

  6. Energy usage for cotton ginning in Australia

    Energy Technology Data Exchange (ETDEWEB)

    Ismail, S.A. [MARA Univ. of Technology, Shah Alam (Malaysia). Faculty of Applied Sciences; Southern Queensland Univ., Toowoomba, QLD (Australia). National Centre for Engineering in Agriculture; Chen, G.; Baillie, C.; Symes, T. [Southern Queensland Univ., Toowoomba, QLD (Australia). National Centre for Engineering in Agriculture

    2010-07-01

    This paper reported on a study that evaluated the energy consumption of cotton gins used in Australia. The average electricity use is 52.3 kWh per bale. In practicality, the electricity consumption for different gins is correlated linearly with the bale numbers produced. The cost of electricity is therefore important in cotton ginning operations. The power factor in all the gins monitored in this study was greater than 0.85. The study showed that the use of gas dryers was highly influenced by the cotton moisture and regulated drying temperature. In general, electricity and gas consumption comprised 61 and 39 per cent of total energy use respectively. The study showed that 60.38 kg of carbon dioxide are emitted for ginning each bale of cotton. This paper described a newly developed method for monitoring the energy performance in cotton gins. Detailed monitoring and analysis carried out at 2 gin sites revealed that electricity consumption is not influenced much by changes in trash content in the module, degree of moisture and lint quality. However, the cotton variety influences the energy consumption. Cotton handling constituted nearly 50 per cent of the power used in both gins.

  7. Electricity use patterns in cotton gins

    Science.gov (United States)

    Energy costs are the second largest source of variable costs for cotton gins, with electricity accounting for 18% of variable costs. Energy use has typically not been a major consideration in gin design and previous studies of energy use have utilized instantaneous readings or aggregated season-lon...

  8. Levels and chemical composition of cotton gin dust.

    Science.gov (United States)

    Wesley, R A; Wall, J H

    1978-12-01

    Dust levels were determined in the three principal work areas of five high-capacity, saw-type cotton gins processing spindle-picked cotton. Dust levels measured by the vertical elutriator, OSHA personal and stationary personal samplers averaged 0.66, 0.96 and 0.87 mg/m3, respectively. Gross chemical analyses of dust samples collected indicated that the composit0n of the dust was highly variable and different for the principal work areas within each gin -- 15 to 53% ash, 2 to 5% moisture, 8 to 18% protein, 19 to 55% cellulose and 8 to 16% water-extractable constituents. Major elements were silicon, potassium, aluminum, calcium and magnesium. PMID:742599

  9. Cotton gin trash: can it be safely utilized

    Energy Technology Data Exchange (ETDEWEB)

    Parnell, C.B.; Emino, E.R.; Grubaugh, E.K.

    1980-08-01

    Ginning creates lots of gin trash with subsequent disposal problems. The Texas A and M University Agricultural Engineering Dept. is conducting a systems engineering study of three utilization alternatives, composting, fuel, and cattlefeed, funded by the National Science Foundation. Arsenic contamination is a major constraint in utilizing cotton gin trash - arsenic acid is used to desiccate the cotton plant before harvesting. Data from growth trials of 12 species of vegetable and ornamental plants grown on composted cotton gin trash are presented. (Refs. 2).

  10. Process steam production from cotton gin trash

    Energy Technology Data Exchange (ETDEWEB)

    LePori, W.A.; Carney, D.B.; Lalk, T.R.; Anthony, R.G.

    1981-01-01

    A steam producing system based on fluidized-bed gasification of biomass materials is discussed. Limited experimental results are discussed and show that steam has been produced at rates of 334.3 kg/hr. (737 lbs/hr.) with 2.8 kg of stream produced for each kilogram of cotton gin trash (2.8 lb/lb.). ref.

  11. PARTICULATE EMISSION PROFILE OF A COTTON GIN

    Science.gov (United States)

    PARTICULATE MATTER (PM) IS ONE OF SIX CRITERIA POLLUTANTS REGULATED BY THE ENVIRONMENTAL PROTECTION AGENCY (EPA) WITH NATIONAL AMBIENT AIR QUALITY STANDARDS (NAAQS). IN GENERAL, PM IS THE ONLY AIR POLLUTANT OF CONCERN EMITTED FROM COTTON GINS. THE EPA HAS NAAQS FOR PM10 (PARTICLES WITH AN AERODYNA...

  12. Interaction between GInB and the N-terminal domain of NifA in Azospirillum brasilense

    Institute of Scientific and Technical Information of China (English)

    ZHOU XiaoYu; ZOU XiaoXiao; LI JiLun

    2008-01-01

    Azospirillum brasilense is a diazotroph associated with many important agricultural crops and shows potential as a biofertilizer. NifA, the transcriptional activator of nitrogen fixation (nif) genes, and GInB, one of P,, signal transduction family protein, are key proteins in the regulation of nitrogen fixation in A. brasilense. It was previously reported that the regulation of NifA activity in A. brasilense depends on GInB. We report here that GInB was found to interact directly with the N-terminal domain of NifA in vivo under nitrogen-free conditions and the N-terminal mutant of NifA in which the Tyr residues at position 18 and 53 were replaced by Phe (NifA-N-Y18/53F) strengthened the interaction with GInB. Moreover, we also found that the amino acid residues 66-88 and 165-176 in N-terminus of NifA are responsible for the interaction with GInB.

  13. Gin-mediated DNA inversion: product structure and the mechanism of strand exchange.

    Science.gov (United States)

    Kanaar, R; van de Putte, P; Cozzarelli, N R

    1988-02-01

    Inversion of the G loop of bacteriophage Mu requires the phage-encoded Gin protein and a host factor. The topological changes in a supercoiled DNA substrate generated by the two purified proteins were analyzed. More than 99% of the inversion products were unknotted rings. This result excludes synapsis by way of a random collision of recombination sites, because the resulting entrapped supercoils would be converted into knots by recombination. Instead, the recombination sites must come together in the synaptic complex in an ordered fashion with a fixed number of supercoils between the sites. The linking number of the substrate DNA increases by four during recombination. Thus, in three successive rounds of inversion, the change in linking number was +4, +8, and +12, respectively. These results lead to a quantitative model for the mechanism of Gin recombination that includes the distribution of supercoils in the synaptic complex, their alteration by strand exchange, and specific roles for the two proteins needed for recombination.

  14. Use of cotton gin trash and compatibilizers in polyethylene composites

    Science.gov (United States)

    The ginning of cotton produces 15-42% of foreign materials, called “cotton gin trash”, including cotton burr, stems, leaf fragment, and dirt. In this work we examined the mechanical properties of composites of low density polyethylene (LDPE) and cotton burr. The burr was ground into powder, and se...

  15. Characterization of cotton gin particulate matter emissions-database development

    Science.gov (United States)

    In 2006, EPA implemented a more rigorous standard for PM2.5 in 2006 and all the cotton gins are or will be impacted by this standard. The primary issues associated with implementing this standard are: 1) very limited cotton gin PM2.5 data are available; 2) sampler errors, recent research indicates t...

  16. Lubbock Gin Lab - Current Research and Leaf Grade Issues

    Science.gov (United States)

    This presentation was given to a group of cotton gin managers and allied industry reps. Approximately 100 attendees were in the audience. A discussion of the current research conducted at the USDA ARS CPPRU Ginning Laboratory in Lubbock, TX was given along with a discussion of leaf grade issues and ...

  17. Evaluation of a mass flow sensor at a gin

    Science.gov (United States)

    As part of a system to optimize the cotton ginning process, a custom-built mass flow sensor was evaluated at USDA-ARS Cotton Ginning Research Unit at Stoneville, Mississippi. The mass flow sensor was fabricated based on the principle of the sensor patented by Thomasson and Sui. The optical and ele...

  18. Changes in cotton gin energy consumption apportioned by ten functions

    Science.gov (United States)

    The public is concerned about air quality and sustainability. Cotton producers, gin owners and plant managers are concerned about rising energy prices. Both have an interest in cotton gin energy consumption trends. Changes in cotton gins’ energy consumption over the past fifty years, a period of ...

  19. Composition and ethanol production potential of cotton gin residues.

    Science.gov (United States)

    Agblevor, Foster A; Batz, Sandra; Trumbo, Jessica

    2003-01-01

    Cotton gin residue (CGR) collected from five cotton gins was fractionated and characterized for summative composition. The major fractions of the CGR varied widely between cotton gins and consisted of clean lint (5-12%),hulls (16-48%), seeds (6-24%), motes (16-24%), and leaves (14-30%). The summative composition varied within and between cotton gins and consisted of ash (7.9-14.6%), acid-insoluble material (18-26%), xylan (4-15%),and cellulose (20-38%). Overlimed steam-exploded cotton gin waste was readily fermented to ethanol by Escherichia coli KO11. Ethanol yields were feedstock and severity dependent and ranged from 58 to 92.5% of the theoretical yields. The highest ethanol yield was 191 L (50 gal)/t, and the lowest was 120 L (32 gal)/t. PMID:12721487

  20. Advances in cotton gin trash energy conversion

    Energy Technology Data Exchange (ETDEWEB)

    Rutherford, R.D.; Parnell, C.B.; Finch, S.F.; Siebold, W.J.

    1985-01-01

    The concept of using agricultural residues, especially cotton gin trash (CGT), as a fuel for a small cogeneration power plant based on fluidized bed gasification (FBG) requires that three problems be solved: (1) ash must be removed from the low calorific value (LCV) gas prior to combustion; (2) the high NO/sub x/ emissions associated with many biomass fuels must be significantly reduced; (3) a systems analyses of engineering/economic feasibility for potential applications must be developed. This paper addresses current research at TAMU pertaining to these problems.

  1. 29 CFR 780.805 - Ginning of “cotton.”

    Science.gov (United States)

    2010-07-01

    ... Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet Molasses, Sugarcane, or Maple Sap into Sugar or Syrup; Exemption From Overtime Pay Requirements Under Section 13(b)(15) Ginning of Cotton for Market § 780.805 Ginning of “cotton.” Only the ginning of “cotton” is within the first part of...

  2. Evaluation of cotton gin byproducts as a ligno-cellulosic filler in polymer composites

    Science.gov (United States)

    The byproduct produced as a result of ginning has created disposal problems across the cottonbelt of the United States for several decades. Research has evaluated numerous potential applications for utilizing cotton gin byproducts (CGB) more commonly referred to by ginners as "gin waste" or "gin tra...

  3. Dust cyclone technology for gins – A literature review

    Science.gov (United States)

    Dust cyclone research leading to more efficient designs has helped the cotton ginning industry to comply with increasingly stringent air quality regulations governing fine particulate emissions. Future changes in regulations may require additional improvements in dust cyclone efficacy. This inter-...

  4. Eco-friendly rubberized cotton fabric roller for ginning machines.

    Science.gov (United States)

    Iyer, G V

    2007-01-01

    This article discusses the pollution caused by chrome composite leather-clad (CCLC) rollers commonly used in cotton roller ginning mills and suggests an alternative roller material. CCLC rollers contain about 18,000 to 36,000 mg/kg (ppm) total chromium in trivalent and hexavalent forms, which are toxic to human health and carcinogenic. When seed-cotton is processed in double roller (DR) ginning machines, the lint is contaminated with chromium, and chromium particles are carried into the spun yarns and cotton by-products. Specifically, due to persistent rubbing of the leather-clad roller over the stationary knife during the ginning process, the lint is contaminated with about 140 to 1990 ppm of chromium, and the spun yarns and cotton by-products contain about 100 to 200 ppm, far in excess of the standard limit of 0.1 ppm. Gin and mill workers are directly exposed to this carcinogenic substance. To offset this problem, pollution-free rubberized cotton fabric (RCF) rollers have been fabricated and tested in roller gins. The RCF roller covering is made of multiple layers of fabric bonded together using a white rubber compound, which has a surface finish conducive to high ginning efficiency. This eliminates chromium contamination and pollution during the ginning process. On the basis of the design and development of various test rollers and subsequent evaluation studies, the performance of pollution-free RCF rollers has been demonstrated with reference to their commercial benefit and eco-friendliness in cotton ginning mills.

  5. Integrative Functional Genomics Analysis of Sustained Polyploidy Phenotypes in Breast Cancer Cells Identifies an Oncogenic Profile for GINS2

    Directory of Open Access Journals (Sweden)

    Juha K. Rantala

    2010-11-01

    Full Text Available Aneuploidy is among the most obvious differences between normal and cancer cells. However, mechanisms contributing to development and maintenance of aneuploid cell growth are diverse and incompletely understood. Functional genomics analyses have shown that aneuploidy in cancer cells is correlated with diffuse gene expression signatures and aneuploidy can arise by a variety of mechanisms, including cytokinesis failures, DNA endoreplication, and possibly through polyploid intermediate states. To identify molecular processes contributing to development of aneuploidy, we used a cell spot microarray technique to identify genes inducing polyploidy and/or allowing maintenance of polyploid cell growth in breast cancer cells. Of 5760 human genes screened, 177 were found to induce severe DNA content alterations on prolonged transient silencing. Association with response to DNA damage stimulus and DNA repair was found to be the most enriched cellular processes among the candidate genes. Functional validation analysis of these genes highlighted GINS2 as the highest ranking candidate inducing polyploidy, accumulation of endogenous DNA damage, and impairing cell proliferation on inhibition. The cell growth inhibition and induction of polyploidy by suppression of GINS2 was verified in a panel of breast cancer cell lines. Bioinformatic analysis of published gene expression and DNA copy number studies of clinical breast tumors suggested GINS2 to be associated with the aggressive characteristics of a subgroup of breast cancers in vivo. In addition, nuclear GINS2 protein levels distinguished actively proliferating cancer cells suggesting potential use of GINS2 staining as a biomarker of cell proliferation as well as a potential therapeutic target.

  6. Feasibility Study of a Multiproduct Biorefinery in West Texas From Using Cotton Gin Waste

    OpenAIRE

    Tangaoui, Abbes; Michael, Farmer

    2014-01-01

    This research is a feasibility study of a multiproduct Biorefinery from using cotton gin waste in West Texas. This region is traditionally known by its important production of upland cotton and where there is an important concentration of gin mills. An important and cheap quantity of cotton gin waste results from the ginning process could be transformed from a liability with respect to environment to a valuable feedstock to produce a portfolio of bio-products in small and distributed biorefin...

  7. Systems simulation of cotton gin waste as a supplemental fuel in a coal powered generating plant

    Energy Technology Data Exchange (ETDEWEB)

    Parnell, C.B. (Texas A and M Univ., College Station); Grubaugh, E.K.; Johnston, M.T.; Ladd, K.L.

    1981-01-01

    A systems simulation model of gin trash use at a Lamb County, Texas, power plant was developed. The model is being used to study gin trash supply, both quantity and transportation, fixed and variable cost, and economic benefit/costs of gin trash utilization. Preliminary results indicate the positive feasibility of using gin trash as a supplemental fuel in a coal fired power plant. (MHR)

  8. Characterization of cotton gin total particulate matter emissions based on EPA stack sampling methodologies

    Science.gov (United States)

    A project to characterize cotton gin emissions in terms of stack sampling was conducted during the 2008 through 2011 ginning seasons. The impetus behind the project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. EPA AP-42 emission factors ar...

  9. Textile processing improvements due to high speed roller ginning of upland cotton

    Science.gov (United States)

    Selective breeding has improved upland cotton fiber properties. Processing capacities of new high-speed roller ginning technology approach that of saw ginning. Spinning mills are interested in mill performance data comparing new upland cultivars processed by both saw and roller ginning. Four dive...

  10. Semana de ginástica: um olhar superador

    Directory of Open Access Journals (Sweden)

    2006-11-01

    Full Text Available Este texto relata a experiência vivenciada na disciplina Ginástica I, do curso de Educação Física, UFG/CAC, com o objetivo de problematizar o desenvolvimento histórico da ginástica em suas muitas variações na atualidade e verificar as possibilidades de sua utilização no espaço escolar a partir da construção coletiva, em uma perspectiva crítico-superadora. PALAVRAS-CHAVE: Ginástica - Educação Física Escolar A week of Gymnastics I was held in order to discuss the historical background of Gymnastics, its great number os varieties, and to check the possibilities of use of the space school as far as a critical perspective is concerned. KEY WORD: Gymnastics – Scholar Physical Education.

  11. Boll weevil (Coleoptera: Curculionidae) survival through cotton gin trash fans.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-10-01

    There is concern that cotton gins may serve as loci for reintroduction of boll weevils, Anthonomus grandis grandis Boheman, to eradicated or suppressed zones when processing weevil-infested cotton from neighboring zones. Previous work has shown that virtually all weevils entering the gin in the seed cotton will be removed before they reach the gin stand. Those not killed by the seed cotton cleaning machinery will be shunted alive into the trash fraction, which passes through a centrifugal trash fan before exiting the gin. The objective of this study was to determine survival potential of boll weevils passed through a trash fan. Marked adult weevils were distributed in gin trash and fed through a 82.6-cm (32.5-in.) diameter centrifugal fan operated across a range of fan-tip speeds. A small number of boll weevils were recovered alive immediately after passage through the fan, but all were severely injured and did not survive 24 h. In another experiment, green bolls infested with both adult- and larval-stage weevils were fed through the fan. Several teneral adults survived 24 h, and there was no evidence that fan-tip speed affected either initial survival of weevils, or the number of unbroken boll locks that could harbor an infesting weevil. Thus, designating a minimum fan-tip speed for ensuring complete kill is not possible for the boll weevil. Experiments suggest that a device installed in a gin that partially crushes or cracks bolls open before entering a trash fan will increase mortality, possibly enough that further precautions would be unnecessary. PMID:15568350

  12. Effecting Affection: The Corporeal Ethics of Gins and Arakawa

    Science.gov (United States)

    Bearn, Gordon C. F.

    2010-01-01

    This paper argues for the educational relevance of the apparently unbelievable project of the architects Arakawa and Gins to design houses that would grant their inhabitants immortality. First, the author demonstrates that, broadly conceived, this is a familiar philosophical project, perhaps "the" philosophical project. He then produces a reading…

  13. Removal of plastic sheet material with normal cotton ginning equipment

    Science.gov (United States)

    US cotton is known to be relatively free of contamination but occasionally pieces of sheet plastic are found in cotton bales produced in the US. To better understand how ginning equipment handles pieces of sheet plastic 5 types of plastic commonly found in US seed cotton fields or used in handling ...

  14. A longitudinal study of pulmonary function in cotton gin workers in the San Joaquin Valley.

    Science.gov (United States)

    Larson, R K; Barman, M L

    1989-10-01

    In a previous study we found no significant effects of acute exposure to the cotton gin environment on FEV1 in San Joaquin Valley gin workers compared with concurrent control subjects, nor was there an excess of obstructive lung disease among the same gin workers. The present study looked at the mean annual decline in FEV1, FEV1/FVC, and FEF25-75% in San Joaquin Valley cotton gin workers vs concurrent control subjects over four years. The FEV1 of gin workers completing all four years of the study declined 27.3 ml vs 35.5 ml in control subjects (p = 0.37); FEV1/FVC declined 0.21 percent in gin workers and 0.23 percent in control subjects (p = 0.86); FEF25-75% declined 0.058 L/s in gin workers and 0.066 L/s in control subjects (p = 0.68). Also, the mean annual rate of change of FEV1 was less among the gin workers who did not complete all four years than the gin workers completing all four years. The presence of "byssinosis" symptoms did not significantly influence the results. This prospective, longitudinal study failed to find any detrimental effect of the cotton gin environment on the rate of decline of FEV1, FEV1/FVC, and FEF25-75%. This result was independent of "byssinosis" symptoms and the effect of "dropouts." PMID:2791678

  15. GINS: a new tool for VLBI Geodesy and Astrometry

    CERN Document Server

    Bourda, G; Biancale, R

    2008-01-01

    In the framework of the "Groupe de Recherches de G\\'eod\\'esie Spatiale" (GRGS), a rigorous combination of the data from five space geodetic techniques (VLBI, GPS, SLR, LLR and DORIS) is routinely applied to simultaneously determine a Terrestrial Reference Frame (TRF) and Earth Orientation Parameters (EOP). This analysis is conducted with the software package GINS which has the capability to process data from all five techniques together. Such a combination at the observation level should ultimately facilitate fine geophysical studies of the global Earth system. In this project, Bordeaux Observatory is in charge of the VLBI data analysis, while satellite geodetic data are processed by other groups. In this paper, we present (i) details about the VLBI analysis undertaken with GINS, and (ii) the results obtained for the EOP during the period 2005-2006. We also compare this EOP solution with the IVS (International VLBI Service for geodesy and astrometry) analysis coordinator combined results. The agreement is at ...

  16. Steam Explosion Pretreatment of Cotton Gin Waste for Fuel Ethanol Production

    OpenAIRE

    Jeoh, Tina

    1998-01-01

    Steam Explosion Pretreatment of Cotton Gin Waste for Ethanol Production By Tina Jeoh Foster A. Agblevor, Chair Biological Systems Engineering ABSTRACT The current research investigates the utilization of cotton gin waste as a feedstock to produce a value-added product - fuel ethanol. Cotton gin waste consists of pieces of burs, stems, motes (immature seeds) and cotton fiber, and is considered to be a lignocellulosic material. The three main chemical constituents are ce...

  17. Seasonal Change of Steric Sea Level in the GIN Seas

    Institute of Scientific and Technical Information of China (English)

    LI Lei; WANG Huijuan; SUN Ruili

    2011-01-01

    The Greenland Sea, Iceland Sea, and Norwegian Sea (GIN seas) form the main channel connecting the Arctic Ocean with other Oceans, where significant water and energy exchange take place, and play an important role in global climate change. In this study steric sea level, associated with temperature and salinity, in the GIN seas is examined based on analysis of the monthly temperature and salinity fields from Polar science center Hydrographic Climatology (PHC3.0). A method proposed by Tabata et al. is used to calculate steric sea level, in which, steric sea level change due to thermal expansion and haline contraction is termed as the thermosteric component (TC) and the halosteric component (SC), recpectively. Total steric sea level (TSSL) change is the sum of TC and SC. The study shows that SC is making more contributions than TC to the seasonal change of TSSL in the Greenland Sea, whereas TC contributes more in the Norwegian and the Iceland Seas. Annual variation of TSSL is larger than 50ram over most regions of the GIN Seas, and can be larger than 200mm at some locations such as 308mm at 76.5°N, 12.5°E and 246mm at 77.50N, 17.5°W.

  18. Energy generation from cotton gin trash: an economic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lacewell, R.D.; Taylor, C.R.; Hiler, E.A.

    1981-01-01

    This study consists of economic analyses of electric power generation and low-Btu (British thermal unit) gas generation from cotton gin trash. Both analyses consider the use of a large gin, sized at 40,000 bales per year. A fluidized-bed combustor is used to produce the low Btu gas and in conjunction with a boiler and turbine to produce electricity. For this case study, the consideration of economic feasibility involves the saving of the cost of energy not purchased, the sale of surplus electricity, and the saving of the cost of gin trash disposal eliminated; all are results of on-site energy generation. Electricity requirements will be satisfied, and waste heat will be used for cotton drying. The savings that would result from these two measures total about $126,000 (based on a 300,000 Btu per bale requirement for cotton drying with natural gas priced at $2.50 per thousand cubic feet and electricity priced at 4 cents per kWh). (MCW)

  19. Fiber and yarn properties from high-speed roller ginning of upland cotton

    Science.gov (United States)

    Selective breeding has improved upland cotton fiber properties. New high-speed roller ginning technology has advanced to the point that processing capacities have approached that of saw ginning. Producers seeking a better price for their upland cotton are interested in fiber property and textile m...

  20. Battery condenser system total particulate emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  1. Battery condenser system PM10 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that to characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study ...

  2. 29 CFR 780.803 - Basic conditions of exemption; first part, ginning of cotton.

    Science.gov (United States)

    2010-07-01

    ... cotton. 780.803 Section 780.803 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION... FAIR LABOR STANDARDS ACT Employment in Ginning of Cotton and Processing of Sugar Beets, Sugar-Beet... Section 13(b)(15) Introductory § 780.803 Basic conditions of exemption; first part, ginning of...

  3. 1st stage mote system total particulate emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  4. Combined mote system total particulate emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  5. Overflow system PM10 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  6. Battery condenser system total particulate emission factors and rates for cotton gins: Method 17

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  7. Mote cleaner system total particulate emission factors and rates for cotton gins: Method 17

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  8. 1st stage lint cleaning system PM10 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  9. Mote trash system total particulate emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  10. Evaluation of an experimental mass-flow sensor of cotton-lint at the gin

    Science.gov (United States)

    As part of a system to optimize the cotton ginning process, a custom built mass-flow sensor was evaluated at USDA-ARS Cotton Ginning Research Unit at Stoneville, Mississippi. The mass-flow sensor was fabricated based on the principle of the senor patented by Thomasson and Sui (2004). The optical a...

  11. Study of respiratory effect of short- and long-term cotton gin exposure.

    Science.gov (United States)

    Larson, R K; Barman, M L; Smith, D W; Nicol, L

    1981-04-01

    No excess of obstructive airway disease was found in a group of 265 cotton gin workers when compared with other San Joaquin Valley agricultural workers. After an average of eight weeks' employment in San Joaquin Valley cotton gins, 125 workers showed no appreciable deterioration of pulmonary function compared with pre-employment measurements. In studies of pulmonary function during a workshift, cotton gin workers showed slightly greater mean decrements than control agricultural workers. These differences did not reach a level of statistical significance and were lower than those usually found in byssinosis. The unusual temporal pattern of employment in cotton gins in California precludes a simple approach to diagnosis by symptoms. No correlation was found in this study between symptoms of byssinosis and objective decrements in FEV1. The questionnaire as proposed by the Cotton Dust Standard was found to be of no value in detecting reactors in this study of gin workers. PMID:7471886

  12. Calculation of pesticide degradation in decaying cotton gin trash.

    Science.gov (United States)

    Crossan, Angus N; Kennedy, Ivan R

    2008-10-01

    Pesticide residues were measured in stockpiled cotton gin trash (CGT) over a 2-year period. Samples were analysed by GC/MS/MS and interpretation of the results was aided by the presence of DDE residues, remnant from prior DDT use. Fourteen pesticide residues from current agricultural practice were detected in CGT. Several of these, including indoxacarb, profenofos, chlorpyrifos, propargite, bifenthrin, ethion and cyhalothrin, were more persistent than expected on the basis of published data for soil dissipation. The results showed a complex pattern of pesticide residue decay over time because of the simultaneous decomposition of the CGT matrix. PMID:18651089

  13. Work health and safety in cotton ginning industry: a survey of practices in australia

    International Nuclear Information System (INIS)

    This survey focuses on the WH and S (Work Health and Safety) practices in Australia and relates them with those in Pakistan. It also highlights the planned areas of work required on WH and S in cotton ginning industry of Pakistan. This article is one a series of research studies that will inform a broader approach development. The aim of the survey was to design a standardized health and safety Act for cotton ginning industry of Pakistan and to help ginners meet their due industry obligations under the model WH and S Act. The first component of the research study survey was to review the relevant Australian work and safety model as this provides a framework to protect the health, safety and welfare of all employees at work and of other people who might be affected by the job. The second aspect of the research study survey concerned site visits to cotton gins with the support of Australian Centre for Agricultural Health and Safety, Moree, NSW. During these visits the existing ginning process in terms of WH and S were reviewed. Informal interviews were also conducted with Gin Managers and Ginning Experts regarding WH and S in the Australian cotton ginning industry. (author)

  14. Cotton gin trash incinerator-air heat project. Consultant report (final)

    Energy Technology Data Exchange (ETDEWEB)

    1980-05-01

    The California Energy Commission has funded the final phase of a four year project resulting in development of a successful system for burning cotton gin trash as a fuel providing the heat for ginning. The incinerator - air heater system installed in Corcoran, California operates continuously throughout the ginning season. Trash feeding and burning rate is automatically controlled from the combustion temperature, hot air temperature is controlled by the drying needs, and ashes are automatically removed from the system and pneumatically conveyed to the disposal site. The system complies with state and county air pollution codes by means of baghouse collectors. Savings in fossil fuel and trash disposal costs have demonstrated the equipment system is feasible for a four year payback at large, well utilized gins.

  15. Boll weevil (Coleoptera: Curculionidae) survival through the seed cotton cleaning process in the cotton gin.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-08-01

    There is concern that gins located in boll weevil, Anthonomus grandis grandis Boheman, eradication zones may become points of reintroduction when they process cotton grown in a neighboring infested area. We estimated boll weevil survival through two typical machine sequences used in commercial cotton gins to clean and dry the seed cotton in advance of the gin stand, as well as separately through two incline cylinder cleaners or one or two tower dryers operating at different temperatures. Large numbers of laboratory-reared adult boll weevils were marked with fluorescent powder, fed into the test system, and recovered with the assistance of blacklights. We found no evidence of survival through the seed cotton cleaning systems even when the dryers were not heated, or when passed separately through the two incline cleaners alone. Upper confidence limits (95%) were calculated for the observed zero recoveries based on sample size and the binomial distribution, and these represent the statistical worst-case (i.e., highest) survival potential. Survival through heated tower dryers declined rapidly to zero at higher temperatures, especially when two dryers were running. Although we conclude that the potential for survival of weevils in the seed cotton to the gin stand is zero or close to zero, a small percentage of live weevils was recovered in the green boll/rock trap, which may represent the greatest threat of reintroduction at the gin. Escape of live weevils with the gin trash is also possible, and studies addressing this issue will be presented elsewhere. PMID:15384344

  16. Da ‘Ginástica para a juventude’ a ‘A ginástica alemã’: observações acerca dos primeiros manuais alemães de ginástica

    Directory of Open Access Journals (Sweden)

    Evelise Amgarten Quitzau

    2015-06-01

    Full Text Available A passagem do século XVIII para o XIX se apresenta como um momento importante na constituição do que hoje conhecemos como educação física, pois é nesse período que as primeiras sistematizações dos exercícios físicos começam a ser formuladas e publicadas em diferentes manuais de ginástica. Na Alemanha, dois manuais tiveram grande destaque nesse momento de consolidação dos sistemas de exercícios físicos: Ginástica para a juventude, publicado em 1793 por J.C.F. Guts Muths, e A ginástica alemã, publicado em 1816 por F.L. Jahn. Este artigo tem como objetivo estabelecer uma aproximação entre esses dois manuais e apresentar os argumentos usados pelos autores para justificar a importância da ginástica, bem como similaridades existentes entre as duas propostas.

  17. Ecological study of the larger black flour beetle in cotton gin trash.

    Science.gov (United States)

    Nansen, Christian; James, Jacob; Bowling, David; Parajulee, Megha N; Porter, Patrick

    2008-12-01

    The larger black flour beetle Cynaeus angustus (Leconte) thrives in cotton gin trash piles on the Southern High Plains of Texas and sometimes becomes a nuisance after invading public and private structures. For better understanding of the basic larger black flour beetle ecology in gin trash piles, we conducted a series of laboratory and semirealistic field trials. We showed (1) in naturally infested gin trash piles, that similar trap captures were obtained in three cardinal directions; (2) in a laboratory study, late-instar larvae stayed longer in larval stage in moist soil compared with drier soil; (3) in both horizontal and vertical choice experiments, late instars preferred soil with low moisture content; and (4) specifically larger black flour beetle adults, but most larvae as well, responded negatively to high moisture content in gin trash. The results presented are consistent with reports of larger black flour beetle living in decaying yucca palms in deserts and suggest that maintaining gin trash piles with high moisture content may be an important component in an integrated control strategy. PMID:19161678

  18. The logic of DNA replication in double-stranded DNA viruses: insights from global analysis of viral genomes

    OpenAIRE

    Kazlauskas, Darius; Krupovic, Mart; Venclovas, Česlovas

    2016-01-01

    Genomic DNA replication is a complex process that involves multiple proteins. Cellular DNA replication systems are broadly classified into only two types, bacterial and archaeo-eukaryotic. In contrast, double-stranded (ds) DNA viruses feature a much broader diversity of DNA replication machineries. Viruses differ greatly in both completeness and composition of their sets of DNA replication proteins. In this study, we explored whether there are common patterns underlying this extreme diversity...

  19. Battery condenser system PM10 emission factors and rates for cotton gins: Method 201A PM10 sizing cyclones

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that to characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study ...

  20. 1st stage seed-cotton cleaning system PM10 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  1. Second stage mote system PM10 emission factors and rates for cotton gins: Method 201A PM10 sizing cyclone

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  2. Theoretical bases of rotation dynamics of raw materials poller with s ratify consideration for saw gin processing of cotton

    International Nuclear Information System (INIS)

    In this paper is examined motion of raw materials rollers on the base of a stratify models. Foe every layers of the rollers are determined dynamical influences for saw gin processing of cotton. It is noticed that in the gin processing of cotton the second layer of cotton roller plagues an important role

  3. Unloading system PM10 emission factors and rates for cotton gins: Method 201A PM10 sizing cyclones

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  4. Second stage seed-cotton cleaning system total particulate emission factors and rates for cotton gins: Method 17

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  5. Preliminary evaluation of feeder and lint slide moisture addition on ginning, fiber quality, and textile processing of western cotton

    Science.gov (United States)

    The objective of this study was to evaluate the effects of moisture addition at the gin stand feeder conditioning hopper and/or the battery condenser slide on gin performance and Western cotton fiber quality and textile processing. The test treatments included no moisture addition, feeder hopper hum...

  6. Tarptautinių santykių disciplinos raida ir ketvirtieji tarpparadigminiai ginčai

    OpenAIRE

    Karpavičiūtė, Ieva

    2009-01-01

    Tarptautinių santykių (TS) disciplinos plėtotę lemia tam tikros teorinės, metodologinės, epistemologinės bei ontologinės mados bei ginčų tarp dominuojančių teorijų bei teorinių prieigų etapai. Tarpparadigminių ginčų (angl. inter-paradigm debate) raidoje ir formavosi tarptautinių santykių disciplina. Galima teigti, jog šie ginčai įvertina disciplinos raidą, tam tikra prasme įprasmina discipliną ir formuoja tolesnes jos plėtotės gaires. Tarptautinių santykių (TS) disciplina (iki šiol) plėtojosi...

  7. The logic of DNA replication in double-stranded DNA viruses: insights from global analysis of viral genomes.

    Science.gov (United States)

    Kazlauskas, Darius; Krupovic, Mart; Venclovas, Česlovas

    2016-06-01

    Genomic DNA replication is a complex process that involves multiple proteins. Cellular DNA replication systems are broadly classified into only two types, bacterial and archaeo-eukaryotic. In contrast, double-stranded (ds) DNA viruses feature a much broader diversity of DNA replication machineries. Viruses differ greatly in both completeness and composition of their sets of DNA replication proteins. In this study, we explored whether there are common patterns underlying this extreme diversity. We identified and analyzed all major functional groups of DNA replication proteins in all available proteomes of dsDNA viruses. Our results show that some proteins are common to viruses infecting all domains of life and likely represent components of the ancestral core set. These include B-family polymerases, SF3 helicases, archaeo-eukaryotic primases, clamps and clamp loaders of the archaeo-eukaryotic type, RNase H and ATP-dependent DNA ligases. We also discovered a clear correlation between genome size and self-sufficiency of viral DNA replication, the unanticipated dominance of replicative helicases and pervasive functional associations among certain groups of DNA replication proteins. Altogether, our results provide a comprehensive view on the diversity and evolution of replication systems in the DNA virome and uncover fundamental principles underlying the orchestration of viral DNA replication. PMID:27112572

  8. Residues of toxaphene, DEF, and paraquat in plant parts and gin waste from a treated cotton field.

    Science.gov (United States)

    Seiber, J N; Winterlin, W L; McChesney, M M

    1979-01-01

    The origin and fate of the gin waste residues of three chemicals--toxaphene, DEF, and paraquat--used frequently in cotton production in California were studied. Residues were determined for foliage and bolls in the live plants, for lint and seed in harvested seed cotton, and for gin waste and its lint and non-lint components, following commercial treatments to the cotton crop. Gin waste residues were followed during five months of open storage to ascertain dissipation rates in a typical waste sample. Relatively high residues of each of the three chemicals persisted at harvest, during storage prior to ginning, and in stored gin waste. The major proportion of gin waste residue was associated with the non-lint components--leaf parts, burrs, stems and other foreign matter. Average stored gin residues in the lint and non-lint components were 13 and 60, 11 and 58, and 5 and 10 ppm for toxaphene, DEF, and paraquat, respectively, during the open storage period. PMID:539830

  9. Battery condenser system PM2.5 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that detail a project to characterize cotton gin emissions from the standpoint of stack and ambient sampling. The impetus behind the project was the 2006 EPA implementation of a more stringent standard for particulate matter less than or equal to 2....

  10. Battery condenser system particulate emission factors for cotton gins: Particle size distribution characteristics

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of total particulate stack sampling and particle size analyses. In 2013, the Environmental Protection Agency (EPA) published a more stringent standard for particulate matter with nominal diameter less than or e...

  11. Detection of plastic and oily contamination in seed cotton at the gin using infra-red

    Science.gov (United States)

    Plastic and oily foreign material in bales of cotton lint poses problems to spinning and textile mills in the form of decreased productivity and products with inferior quality. Detecting and removing this plastic and oily foreign material at the gin is a long term goal of the cotton industry. In t...

  12. Development of cotton gin PM10 emission factors for EPA’s AP-42

    Science.gov (United States)

    The Compilation of Air Pollution Emission Factors (AP-42) emission factors are assigned ratings, from A (Excellent) to E (Poor), based on the quality of data used to develop them. All current PM10 cotton gin emission factors received quality ratings of D or lower. In an effort to improve these ratin...

  13. Characterization of cotton gin particulate matter emissions - Final year of field work

    Science.gov (United States)

    Due to EPA’s implementation of more stringent standards for particulate matter (PM) with an effective diameter less than 2.5 microns (PM2.5), the cotton ginners’ associations across the cotton belt agreed that there is an urgent need to collect gin emission data. The primary issues surrounding PM re...

  14. 29 CFR 780.807 - Cotton must be ginned “for market.”

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Cotton must be ginned âfor market.â 780.807 Section 780.807 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR STATEMENTS OF GENERAL POLICY OR INTERPRETATION NOT DIRECTLY RELATED TO REGULATIONS EXEMPTIONS APPLICABLE TO AGRICULTURE, PROCESSING OF...

  15. Mote robber system PM2.5 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that detail a project to characterize cotton gin emissions from the standpoint of stack and ambient sampling. The impetus behind the project was the 2006 EPA implementation of a more stringent standard for particulate matter less than or equal to 2....

  16. The Effects of Three Module Types on Cotton Ginning and Fiber Quality

    Science.gov (United States)

    Traditionally seed cotton has been stored in modules from the time it was harvested until it was ginned. These modules have been formed using additional equipment and operators. In an effort to improve the efficiency of cotton production by reducing the number of operators and equipment, two newer...

  17. Biobased composition boards made from cotton gin and guayule watse: Select physical and mechanical properties

    Science.gov (United States)

    Vast quantities of cotton gin byproducts (CGB) are being produced annually. Similarly, guayule wastes after rubber latex production (guayule bagasse) is expected to increase as this industry begins to expand. Use of these waste materials into value-added products can help the economics of the crops,...

  18. Field trial of diatomaceous earth in cotton gin trash against the larger black flour beetle, Cynaeus angustus (Coleoptera: Tenebrionidae).

    Science.gov (United States)

    McIntyre, N E; Porter, P

    2004-04-01

    The larger black flour beetle, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), is an agricultural and home nuisance pest in North America. In the Southern High Plains of Texas, the larger black flour beetle is associated with cotton gin trash, by-products of cotton ginning that are field stored in large piles for economic reasons. Larger black flour beetle overwinter in gin trash piles but may disperse by the millions in summer and autumn, entering houses as far as 2 km away where they cause distress to homeowners. Because > 1.2 billion kg of gin trash is produced annually in Texas alone, the potential consequences of the larger black flour beetle are enormous. We conducted a field experiment that evaluated the efficacy of diatomaceous earth (DE) on the abundance of the larger black flour beetle in gin trash. There were no significant differences in numbers of larger black flour beetle among treatments and controls (mean number of adults summed over time: controls = 115.41, layered treatment = 87.60, top and bottom treatment = 96.50, bottom treatment = 115.16). There were sufficient numbers of beetles in treated piles to still pose a potential home nuisance problem, likely because the moisture content of field-stored gin trash is too high for DE to work effectively. Therefore, treating cotton gin trash with diatomaceous earth will probably be unable to prevent home infestations of larger black flour beetle. Location within a gin trash pile and season influenced pest numbers, which has implications for long-term field storage of cotton gin trash. PMID:15154486

  19. A study of respiratory function among the workers engaged in ginning processes

    Directory of Open Access Journals (Sweden)

    Asim Saha

    2014-01-01

    Full Text Available Background: Known respiratory health effects of exposure to cotton dust are mainly altered pulmonary function findings and symptom of chest tightness. A number of studies have been carried out all over the world to enumerate and evaluate the health effects of cotton dust exposed workers in different processes. However, such studies carried out in ginning industry especially in Indian context are scanty. Objectives: This study was initiated to explore occupational and morbidity details and respiratory functional status of the exposed workers as well as to investigate across the working shift pulmonary function changes. Materials and Methods: A cross-sectional study was conducted including workers from ginning units where principal exposure is from cotton dust. All the workers of the selected ginning units were subjected to an interview with a predesigned questionnaire to collect occupational and medical history, clinical examination and pulmonary function test. Results: In this present study, no cross-shift significant change in relation to PEFR and FEV 1 values is observed. However, chronic effect on lung function is observed in a few subjects and declining trend of values was observed with increasing job duration as well as age of workers and among smokers. Other health problems among these subjects were backache and joint pain. Conclusions: Studies on cotton textile workers have shown both cross-shift and chronic decline of values. In this study on ginning workers, chronic effect only is observed. This difference of observation may be explained by different nature of exposure in case of ginning. This study recommends regular periodic clinical examination, lung function test and monitoring of dust, gram-negative bacteria and endotoxins in such workplaces.

  20. An investigation of RSN frequency spectra using ultra-fast generalized inverse imaging (GIN

    Directory of Open Access Journals (Sweden)

    Rasim eBoyacioglu

    2013-04-01

    Full Text Available With the advancements in MRI hardware, pulse sequences and reconstruction techniques, many low TR sequences are becoming more and more popular within the fMRI community. In this study, we have investigated the spectral characteristics of resting state networks (RSNs with a newly introduced ultra fast fMRI technique, called Generalized Inverse Imaging (GIN. The high temporal resolution of GIN (TR=50 ms enables to sample cardiac signals without aliasing into a separate frequency band from the BOLD fluctuations. Respiration related signal changes are, on the other hand, removed from the data without the need for external physiological recordings. We have observed that the variance over the subjects is higher than the variance over RSNs.

  1. Sister Chromatid Exchange Frequency in Lymphocytes Cultured from Cotton Gin Workers

    OpenAIRE

    ATMACA, Münevver; BAĞCI, Hüseyin; AÇIKBAŞ, İbrahim; GÜMÜŞ, Dilihan; DÜZCAN, Füsun

    2004-01-01

    Genetic biomonitoring of human populations exposed to potential mutagens/carcinogens can be performed using different genetic markers. Sister chromatid exchange (SCE) is one of the most extensively used markers of the early biological effects of DNA damaging agents. In order to assess the genotoxicity associated with exposure to cotton dust, we determined SCE frequency in peripheral blood lymphocytes cultured from 20 cotton gin workers and 20 controls. Student’s-t test indicated an ...

  2. Modeling and Production of Bioethanol from Mixtures of Cotton Gin Waste and Recycled Paper Sludge

    OpenAIRE

    Shen, Jiacheng

    2008-01-01

    In this study, the hydrolytic kinetics of mixtures of cotton gin waste (CGW) and recycled paper sludge (RPS) at various initial enzyme concentrations of Spezyme AO3117 and Novozymes NS50052 was investigated. The experiments showed that the concentrations of reducing sugars and the conversions of the mixtures increased with increasing initial enzyme concentration. The reducing sugar concentration and conversion of the mixture of 75% CGW and 25% RPS were higher than those of the mixture of 80% ...

  3. A MARKOV CHAIN ANALYSIS OF STRUCTURAL CHANGES IN THE TEXAS HIGH PLAINS COTTON GINNING INDUSTRY

    OpenAIRE

    Ethridge, Don E.; Roy, Sujit K.; Myers, David W.

    1985-01-01

    Markov chain analysis of changes in the number and size of cotton gin firms in West Texas was conducted assuming stationary and non-stationary transition probabilities. Projections of industry structure were made to 1999 with stationary probability assumptions and six sets of assumed conditions for labor and energy costs and technological change in the non-stationary transition model. Results indicate a continued decline in number of firms, but labor, energy, and technology conditions alter t...

  4. Ethanol production from cotton gin trash using optimised dilute acid pretreatment and whole slurry fermentation processes.

    Science.gov (United States)

    McIntosh, S; Vancov, T; Palmer, J; Morris, S

    2014-12-01

    Cotton ginning trash (CGT) collected from Australian cotton gins was evaluated for bioethanol production. CGT composition varied between ginning operations and contained high levels of extractives (26-28%), acid-insoluble material (17-22%) and holocellulose (42-50%). Pretreatment conditions of time (4-20 min), temperature (160-220 °C) and sulfuric acid concentration (0-2%) were optimised using a central composite design. Response surface modelling revealed that CGT fibre pretreated at 180 °C in 0.8% H2SO4 for 12 min was optimal for maximising enzymatic glucose recoveries and achieved yields of 89% theoretical, whilst the total accumulated levels of furans and acetic acid remained relatively low at <1 and 2 g/L respectively. Response surface modelling also estimated maximum xylose recovery in pretreated liquors (87% theoretical) under the set conditions of 150 °C in 1.9% H2SO4 for 23.8 min. Yeast fermentations yielded high ethanol titres of 85%, 88% and 70% theoretical from glucose generated from: (a) enzymatic hydrolysis of washed pretreated fibres, (b) enzymatic hydrolysis of whole pretreated slurries and (c) simultaneous saccharification fermentations, respectively. PMID:25280112

  5. Third stage seed-cotton cleaning system PM10 emission factors and rates for cotton gins: Method 201A PM10 sizing cyclones

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that characterize cotton gin emissions from the standpoint of stack sampling. The impetus behind this project was the urgent need to collect additional cotton gin emissions data to address current regulatory issues. A key component of this study was...

  6. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses.

  7. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): a nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Nansen, Christian; Stokes, Bryan; James, Jacob; Porter, Patrick; Shields, Eilson J; Wheeler, Terry; Meikle, William G

    2013-04-01

    The larger black flour beetles, Cynaeus angustus (LeConte) (Coleoptera: Tenebrionidae), feeds on saprophytic fungi found in gin trash piles and occasionally becomes a nuisance pest in adjacent homes and businesses. The potential of Steinernema carpocapsae 'NY 001,' as a potential control agent of larger black flour beetle under experimental conditions was examined with particular reference to the importance of soil moisture content. Without prospects of insecticides being labeled for control of larger black flour beetle in gin trash, the data presented here support further research into applications of entomopathogenic nematodes underneath gin trash piles as a way to minimize risk of larger black flour beetle populations causing nuisance to nearby homes and businesses. PMID:23786050

  8. Bulímicas: elas vão à academia de ginástica?

    Directory of Open Access Journals (Sweden)

    Andréia Weis

    2013-09-01

    Full Text Available O objetivo da pesquisa foi determinar a prevalência de mulheres com sintomas de bulimia nervosa frequentadoras de academia de ginástica e verificar, dentre as praticantes que apresentam traços bulímicos, qual a frequência de utilização do exercício físico como forma compensatória. Participaram do estudo 197 mulheres matriculadas em academias de ginástica da cidade de Marechal Cândido Rondon - PR (28,7±9,9 anos. Para avaliar o quadro de bulimia utilizou-se o Teste de Investigação Bulímica de Edingurgh (BITE e uma anamnese estruturada pelos pesquisadores para atender interesses do estudo. Na análise dos dados foi utilizada a estatística descritiva e a frequência amostral pelo programa estatístico SPSS for Windows 11.5. A prevalência de sintomas de bulimia foi de 3%. A prevalência de traços bulímicos foi de 11,2%. Dos indivíduos que apresentaram traços bulímicos, 59,1% afirmaram praticar exercício físico após episódios de alimentação exagerada. Estes resultados podem indicar que esse comportamento compensatório através da prática de exercício físico pode levar as mulheres a procurar as academias de ginástica.

  9. Ginés Pérez y su O Crux

    Directory of Open Access Journals (Sweden)

    Climent, José

    2002-12-01

    Full Text Available The exact names attributed to Ginés Pérez were never at all clear. His first appointment to the Cathedral in Valencia complicated matters even more when he was referred to with the Christian name of what appeared to be "Juan", which only added to the confusion as it lead us to believe that he was the only candidate to apply for that position. Today, however, we now know his true and full names: he was called Ginés Pérez de la Parra. We also know, for the first time, the exact date of his death. The quality of his compositions not only contributed to their popularity but gave rise to the works of other composers being attributed to him. The authorship of the majority of his compositions are considered anonymous, though they can be located in diverse archives. Even so, we cannot find a single copy of the O Crux attributed to his name, despite the numerous copies and their variants that abound in Region of Valencia.El nombre de Ginés Pérez nunca ha tenido la claridad de debía. Su primer nombramiento en la catedral de Valencia ya lo confunde añadiéndole el “Juan” lo que ya siembra confusión, además de sugerirnos que, posiblemente, fue llamado para ocupar el puesto sin oposición. Hoy conocemos toda su filiación: Ginés Pérez de la Parra, así como la fecha de su muerte, totalmente equivocada hasta ahora. La valía de sus composiciones motivó, además de la difusión de su obra, la atribución de composiciones de otros autores. La mayor parte de sus composiciones constan como "anónimos", si bien pueden encontrarse en distintos archivos. Del presente O crux, sin embargo, no hemos podido encontrar ninguna copia con su nombre, pese a las múltiples copias, con sus variantes, existentes en la Comunidad Valenciana.

  10. Múltiplos itinerários de um lente e diretor do Ginásio Paranaense

    Directory of Open Access Journals (Sweden)

    Serlei Maria Fischer Ranzi

    2012-07-01

    Full Text Available  A partir de uma documentação pertencente ao acervo pessoal do lente e diretor do Ginásio Paranaense entre os anos de 1920 e 1928, Lysímaco Ferreira da Costa, buscamos analisar enunciados escritos que expressam manifestações do diálogo entre o diretor, professores, familiares e alunos, examinando a tensão entre a gestão da escola e o controle externo sobre os procedimentos que nela se desenvolviam. Passando por vários cargos públicos, trazendo suas experiências para a rede escolar, o Diretor defendeu mudanças que geraram controvérsias como, por exemplo, a adoção da disciplina escolar, a vigilância, e o cumprimento de regras do regimento escolar como princípios de gestão. Foi nomeado o homem de sete instrumentos, termo vinculado à imagem construída que procura referenciar uma multiplicidade de cargos assumidos simultaneamente, na sua performance de homem público.Palavras-chave: ensino secundário, ginásio paranaense, gestão escolar.   Abstract Starting by personal documents of the teacher and principal of Ginásio Paranaense, between 1920 and 1928, Lysimaco Ferreira da Costa, we intend to make an analysis of the written talks that express dialogical manifestations among the principal, the teachers, the parents and the pupils, examining the tension between the school management and the external control of the procedures that happened in there. Going through many public posts, bringing experiences to the school system, the principal defended changes that generated controversies as, for example, the adoption of the school discipline, the vigilance, and the attainment of the rules of the school regiment as management principles. He was nominated the man of seven instruments, term all ached to the built image that aims to refer the multiple posts assumed simultaneously, in his performance of public man. Keywords: secundary teachings, ginásio paranaense, school management. 

  11. Topping cogeneration feasibility study for the Central Valley Cooperative, Inc. cotton gin in Hanford, California

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, F.E.

    1980-08-01

    To define baseline operation, a detail measurement program was conducted to determine the detail character of electrical and thermal energy consumption in the gin. Much of the air moving equipment was found to be operating at low efficiencies. A conservation plan was developed, and energy consumption and energy costs were forecast for the future. Fuel supply alternatives were examined as were several prime mover engine technologies. Environmental and regulatory constraints were examined, and all systems examined were subjected to an economic analysis. Key results are discussed.

  12. Potential for escape of live boll weevils (Coleoptera: Curculionidae) into cottonseed, motes, and cleaned lint at the cotton gin.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Baker, Roy V

    2004-12-01

    Reintroductions of the boll weevil, Anthonomus grandis grandis Boheman, into areas of the United States where it has been eradicated or suppressed are very expensive to mitigate. There is concern that a cotton gin in an eradication zone may serve as a site of boll weevil reintroductions when processing cotton harvested in a neighboring infested zone. Similarly, there is a question whether weevil-free areas can safely import gin products, such as cottonseed and baled lint, from infested areas without risking an introduction. Many countries require fumigation of imported U.S. cotton bales to protect against boll weevil introductions, costing the U.S. cotton industry millions of dollars annually. In previously reported experiments, we quantified the potential for boll weevils to survive passage through precleaning machinery in the gin. In this study, we quantified survival potential of boll weevils passing through the gin stand and segregating into the cottonseed, mote, or lint fractions. We also examined boll weevil survival when passed with ginned lint through a lint cleaner. We present a flow chart of experimentally determined survival potentials of boll weevils passing through the various subprocesses of the gin, from which one can calculate the risk of a live boll weevil reaching any point in the process. Our data show that there is virtually no chance of a boll weevil being segregated alive into the cottonseed or of one surviving in the lint to approach the bale press. Therefore, quarantine or fumigation of cottonseed and cotton bales to guard against boll weevil introductions is unnecessary. PMID:15666727

  13. Potential for transport of boll weevils (Coleoptera: Curculionidae) to the cotton gin within cotton modules.

    Science.gov (United States)

    Sappington, Thomas W; Brashears, Alan D; Parajulee, Megha N; Carroll, Stanley C; Arnold, Mark D; Norman, John W; Knutson, Allen E

    2004-06-01

    There is concern that cotton gins located in boll weevil, Anthonomus grandis grandis Boheman, eradication zones serving customers in adjacent infested zones may serve as a site for boll weevil reintroductions if weevils are transported alive inside cotton modules. We surveyed fields in three distinct areas of Texas and found that weevils can be present in large numbers in cotton fields that have been defoliated and desiccated in preparation for harvest, both as free adults and as immatures inside unopened bolls. Harvested cotton taken from module builders indicated that approximately = 100-3700 adult boll weevils were packed inside modules constructed at the sampled fields. Marked weevils were forced through a laboratory field cleaner (bur extractor) commonly mounted on stripper-harvesters, and 14% were recovered alive in the seed cotton fraction and lived at least to 24 h. Survival of weevils placed inside modules declined over time up to 7 d, but the magnitude of the decline varied with experimental conditions. In one experiment, 91% of the weevils survived to 7 d, whereas under harsher environmental conditions, only 11% survived that long. Together, our results indicate that when cotton is harvested in an infested area, boll weevils likely will be packed alive into cotton modules, and many will still be alive by the time the module is fed into the gin, at least up to 7 d after the module's construction. PMID:15279274

  14. Update on the development of cotton gin PM2.5 emission factors for EPA's AP-42

    Science.gov (United States)

    A cotton ginning industry-supported project was initiated in 2008 to update the U.S. Environmental Protection Agency’s (EPA) Compilation of Air Pollution Emission Factors (AP-42) to include PM2.5 emission factors. This study develops emission factors from the PM2.5 emission factor data collected fro...

  15. Eli Whitney's Patent for the Cotton Gin. The Constitution Community: Revolution and the New Nation (1754-1820s).

    Science.gov (United States)

    Schur, Joan Brodsky

    This lesson focuses on the power of the U.S. Congress to pass laws related to issuing patents. Using Eli Whitney's 1812 Congressional petition to extend the patent on his cotton gin as an example, the lesson correlates to the National History Standards and the National Standards for Civics and Government. It contains two primary source documents,…

  16. 2nd stage lint cleaning system PM2.5 emission factors and rates for cotton gins

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that detail a project to characterize cotton gin emissions from the standpoint of stack and ambient sampling. The impetus behind the project was the 2006 EPA implementation of a more stringent standard for particulate matter less than or equal to 2....

  17. Biological control agent of larger black flour beetles (Coleoptera: Tenebrionidae): A nuisance pest developing in cotton gin trash piles.

    Science.gov (United States)

    Larger black flour beetles (LBFB), Cynaeus angustus, feed on saprophytic fungi found in gin trash piles, and become nuisance pests in homes and businesses. We examined the dose-response of three entomopathogenic nematode species (Steinernema carpocapsae, S. feltiae, and Heterorhabditis bacteriophora...

  18. Genetic analysis for some ginning and fiber characteristics in upland cotton

    International Nuclear Information System (INIS)

    Four varieties viz., Chandi-95, CIM-448, NIAB-Karishma and NIAB-78 were crossed in complete diallel fashion(4 x 4) to determine gene action for ginning out turn percentage (GOT), staple length, micronaire value and lint index. Results demonstrated that GOT percentage was controlled by complete dominance whereas, staple length, micronaire value and lint index were conditioned by partial dominance type of gene action. Chandi-95 had maximum dominant genes for staple length, micronaire value and lint index, while NIAB-78 had maximum dominant genes for GOT % and lint index. The variety NIAB-Karishma recorded the highest array means for GOT % and micronaire values followed by Chandi-95 and NIAB-78 for staple length and lint index, respectively. Whereas, cross combination NIAB-Karishma x NIAB-78 manifested highest values within array means for GOT %, micronaire value and lint index indicating good potential for the improvement of these characters. (author)

  19. Sorption and desorption of endosulfan sulfate and diuron to composted cotton gin trash.

    Science.gov (United States)

    Burns, Mitchell; Crossan, Angus N; Kennedy, Ivan R; Rose, Michael T

    2008-07-01

    This paper investigates the potential use of composted cotton gin trash (CCGT) as a pesticide sorption medium in remediation of contaminated tailwater. CCGT was found to contain a large organic matter fraction (25.22%). Sorption of endosulfan sulfate and diuron, using the batch equilibrium method, was rapid but not limited for the range of applied concentrations, with diuron failing to reach equilibrium after two days. The partition K d and organic carbon partition K(OC) coefficients determined diuron ( Kd = 78; K(OC) = 526) and endosulfan sulfate ( Kd = 1500; K(OC) = 10,111) to reside in the solid phase. Limited desorption of diuron and higher range concentrations of endosulfan sulfate (50-100 microg L(-1)) were quantified. Sorption and desorption resulted from hydrophobic and hydrophilic interactions with the humic components of the compost. CCGT was concluded to have a superior sorption capacity to other sorbents reported in the literature, an assessment that requires field substantiation. PMID:18543928

  20. "O trabalho na forma de alegria juvenil": a ginástica segundo Johann Christoph Friedrich Guts Muths

    Directory of Open Access Journals (Sweden)

    Evelise Amgarten Quitzau

    2012-06-01

    Full Text Available Este trabalho tem como objetivo estabelecer uma aproximação com a obra de Johann Christoph Friedrich Guts Muths, pedagogo alemão que, no final do século XVIII, publicou uma das obras fundamentadoras da ginástica alemã: Gymnastik für die Jugend (Ginástica para a Juventude. Seu trabalho baseia-se principalmente nas teorias da denominada polícia médica, e apresenta um forte discurso em defesa da instituição dos exercícios físicos como instrumento de regeneração e fortalecimento da população alemã.

  1. Determinação de padrões de desistência em ginásios

    OpenAIRE

    Pinheiro, Paulo; Cavique, Luís

    2015-01-01

    O problema da retenção e das causas que levam à desistência da frequência do ginásio é uma questão que os ginásios e academias de fitness tentam há muito entender e consequentemente evitar. Tendo em atenção que a indústria do Fitness tem apostado na instalação massificada de sistemas avançados de Customer Relationship Management (CRM) existem atualmente bases de dados com dados históricos de grande valia. Este projeto tem por objetivo aplicar técnicas de classificação a estas bases de dados d...

  2. Processing cotton gin trash to enhance in vitro dry matter digestibility in reduced time.

    Science.gov (United States)

    Pordesimo, Lester O; Ray, Samuel J; Buschermohle, Michael J; Waller, John C; Wilkerson, John B

    2005-01-01

    Cotton gin trash (CGT) in the raw form is poorly digested by ruminants due to lignocellulosic complexes. These structures must be broken down before adequate digestion can occur. This may be performed by physical and/or chemical means. Two methods for improving digestibility are particle size reduction and/or treatment with sodium hydroxide (NaOH). To evaluate the effectiveness of each method, three experiments were performed in which different CGT types were tested. Each type represented trash from a particular cleaning stage in the cotton ginning process. First, each type was ground with a knife-type grinding mill using screen sizes 0.5, 1.0, and 2.0 mm. For the second experiment, particle size was held constant at 2 mm, and all CGT types were treated with 4% and 6% NaOH (w/w) at room temperature. An agitation cycle of 5 min on and 10 min off was used, with the total mixing time being 4 h. Lastly, particle size and NaOH concentration were held constant, and treatments were performed at room temperature, 40 degrees C, and 50 degrees C. The total mixing times were 2 and 3 h for 50 and 40 degrees C, respectively. For all experiments two subsamples of each treatment were tested for in vitro dry matter digestibility (IVDMD). From grinding alone, digestibility increased as particle size decreased. Grinding to 0.5 mm resulted in an average IVDMD of 47.8% while grinding to 2.0 mm resulted in an average IVDMD of only 33.8%. Digestibility also improved with a greater NaOH concentration. An average in vitro digestibility of 70.5% was achieved with 6% NaOH (w/w) treatment, essentially doubling that of the raw CGT. Increasing the reaction temperature did not result in increased digestibility because the mixture dried out, with a consequent reduction in chemical distribution and uniformity in heat transfer. There are still chemical residues in the CGT, and elimination/reduction of these is an issue that needs to be addressed in further research. PMID:15364079

  3. Use of cotton gin trash to enhance denitrification in restored forested wetlands

    Science.gov (United States)

    Ullah, S.; Faulkner, S.P.

    2006-01-01

    Lower Mississippi Valley (LMV) has lost about 80% bottomland hardwood forests, mainly to agriculture. This landscape scale alteration of the LMV resulted in the loss of nitrate (NO3) removal capacity of the valley, contributing to nitrogen (N)-enhanced eutrophication and potentially hypoxia in the northern Gulf of Mexico. Restoration of hardwood forests in the LMV is a highly recommended practice to reduce NO3 load of the Mississippi River. However, restored bottomland forests take decades to develop characteristic ecological functions including denitrifier activity. One way to enhance denitrifier activity in restored wetland forests is to amend the soils with an available carbon (C) source. This research investigated the effects of cotton gin trash (CGT) amendment on denitrification rate and N2O:N2 emission ratio from a restored bottomland forest soils and compared it to those from an adjacent unamended natural forest soils. CGT amendment increased denitrification rates in the restored forest soils to the level of the natural forest soils. N2O:N2 emission ratios from the restored and natural forest soils were highly variable and were not significantly different from each other. These findings suggest that restoration of bottomland hardwood forests in the LMV will require organic carbon amendment to achieve enhanced denitrifier activity for NO3 removal while the restored forest is developing into a mature state over time. ?? 2006 Elsevier B.V. All rights reserved.

  4. Resolução temporal: desempenho de escolares no teste GIN - Gaps-in-noise Temporal resolution: performance of school-aged children in the GIN - Gaps-in-noise test

    Directory of Open Access Journals (Sweden)

    Maria Isabel Ramos do Amaral

    2010-12-01

    Full Text Available A habilidade auditiva denominada resolução temporal consiste no tempo mínimo necessário para resolver eventos acústicos, sendo fundamental para a compreensão de fala, e pode ser avaliada por testes de detecção de gaps, dentre eles o teste GIN - Gaps In Noise. OBJETIVO: Verificar o desempenho da resolução temporal em crianças sem queixas auditivas e/ou dificuldades escolares, no teste GIN, considerando-se o gênero masculino e feminino e a faixa etária de 8, 9 e 10 anos. FORMA DO ESTUDO: Coorte contemporânea com corte transversal prospectivo. MATERIAL E MÉTODO: O teste GIN foi aplicado em 75 escolares, reunidos em três grupos por faixa etária. RESULTADOS: Não foram encontradas diferenças significantes em relação às variáveis orelha e faixa etária. O gênero masculino obteve desempenho levemente melhor do que o feminino em relação apenas à porcentagem de acertos. CONCLUSÃO: A média do limiar de detecção de gaps e porcentagem de acertos foram calculados independente das variáveis orelha, gênero e faixa etária, sendo encontrados os valores de 4,7ms e 73,6%. Baseado no critério de intervalo de confiança 95% como corte para normalidade, os valores do limiar de detecção de gap e porcentagem de acertos foram 6,1ms e 60%, respectivamente.Time resolution hearing skill is the minimum time necessary to solve acoustic events, which is fundamental for speech understanding, and which may be assessed by gap-detection tests, such as the Gaps-in-noise test (GIN. AIM: the purpose of this study was to verify the performance of time processing ability in children with no hearing and/or education difficulties by applying the GIN test in both genders and ages from 8 to 10 years. STUDY DESIGN: a prospective cross-sectional contemporary cohort. MATERIAL AND METHOD: The GIN test was applied to 75 school-aged children separated into three groups by age. RESULTS: The findings showed no statistical differences among age groups or ears

  5. Georges Demeny e Fernando de Azevedo: uma ginástica científica e sem excessos (Brasil, França, 1900-1930

    Directory of Open Access Journals (Sweden)

    Edivaldo Góis Junior

    2015-06-01

    Full Text Available Este trabalho teve como objetivo comparar textos produzidos no Brasil e na França sobre os métodos da ginástica. Para isso, foi feita uma pesquisa histórica limitada às décadas de 1900 a 1930. As fontes analisadas foram os textos de Georges Demeny e Fernando de Azevedo. Concluiu que nas produções textuais dos dois escritores existia a defesa de uma ginástica científica por meio de uma prática controlada e moderada. Contudo, o debate com outras tradições produziu uma cultura de ginástica eclética nos dois países.

  6. Measuring whole plant CO2 exchange with the environment reveals opposing effects of the gin2-1 mutation in shoots and roots of Arabidopsis thaliana.

    Science.gov (United States)

    Brauner, Katrin; Stutz, Simon; Paul, Martin; Heyer, Arnd G

    2015-01-01

    Using a cuvette for simultaneous measurement of net photosynthesis in above ground plant organs and root respiration we investigated the effect of reduced leaf glucokinase activity on plant carbon balance. The gin2-1 mutant of Arabidopsis thaliana is characterized by a 50% reduction of glucokinase activity in the shoot, while activity in roots is about fivefold higher and similar to wild type plants. High levels of sucrose accumulating in leaves during the light period correlated with elevated root respiration in gin2-1. Despite substantial respiratory losses in roots, growth retardation was moderate, probably because photosynthetic carbon fixation was simultaneously elevated in gin2-1. Our data indicate that futile cycling of sucrose in shoots exerts a reduction on net CO2 gain, but this is over-compensated by the prevention of exaggerated root respiration resulting from high sucrose concentration in leaf tissue.

  7. BIL&GIN: A neuroimaging, cognitive, behavioral, and genetic database for the study of human brain lateralization.

    Science.gov (United States)

    Mazoyer, B; Mellet, E; Perchey, G; Zago, L; Crivello, F; Jobard, G; Delcroix, N; Vigneau, M; Leroux, G; Petit, L; Joliot, M; Tzourio-Mazoyer, N

    2016-01-01

    We report on a database, named BIL&GIN, designed for investigating the cognitive, behavioral, genetic, and brain morphological/functional correlates of hemispheric specialization. The database contains records from a sample of 453 adult participants enriched in left-handers (45%, N=205) as compared to the general population. For each subject, socio-demographic data, hand and eye laterality, family handedness, and cognitive abilities in the language, motor, visuo-spatial, and numerical domains have been recorded. T1-MRI and DTI data were also acquired, as well as resting-state functional MRI. Task-evoked functional MRI was performed in a sub-sample of 303 subjects (157 left-handers) using a customized functional battery of 16 cognitive tasks exploring the same three cognitive domains. Performances at the tasks executed in the magnet as well as post-acquisition debriefing were recorded. A saliva sample was obtained from the subjects of this sub-sample from which DNA was extracted. The BIL&GIN contains results of imaging data processing for each subject, namely maps of tissue (GM, WM, CSF) probability, cortical thickness, cortical surface, and diffusion parameters as well as regional values of these phenotypes for regions of both AAL and FreeSurfer parcellations. For the subjects who underwent FMRI, individual SPM contrast maps for each of the 8 runs were also calculated and included in the database, as well as corresponding BOLD variations in ROIs of the AAL and AICHA atlases, and Wilke's hemispheric functional lateralization index. The BIL&GIN data sharing is based on a collaborative model.

  8. Ethanol production of semi-simultaneous saccharification and fermentation from mixture of cotton gin waste and recycled paper sludge

    OpenAIRE

    Shen, Jiacheng; Agblevor, Foster A.

    2010-01-01

    Ethanol production from the steam-exploded mixture of 75% cotton gin waste and 25% recycled paper sludge in various conditions was investigated by semi-simultaneous saccharification and fermentation (SSSF) consisting of a pre-hydrolysis and a simultaneous saccharification and fermentation (SSF). Four cases were studied: 24-h pre-hydrolysis + 48-h SSF (SSSF 24), 12-h pre-hydrolysis + 60-h SSF (SSSF 12), 72-h SSF, and 48-h hydrolysis + 24-h fermentation (SHF). The ethanol concentration, yield, ...

  9. Gasification of oak sawdust, mesquite, corn stover, and cotton gin trash in a countercurrent fluidized bed pilot reactor

    Energy Technology Data Exchange (ETDEWEB)

    Beck, S.R.; Wang, M.J.; Hightower, J.A.

    1981-01-01

    The Synthesis Gas From Manure (SGFM) process was designed to convert cattle feedlot manure to ammonia synthesis gas. Current work is aimed at using any biomass feedstock to produce either medium-Btu gas or chemical feedstocks. This paper presents a comparison of the experimental results compiled on gasification of oak sawdust, corn stover, mesquite, and cotton gin trash in the SGFM pilot plant. A weighted comparison of the product gas, hydrocarbon, and hydrogen yields, gas quality, calorific value of product gas, percentage conversion of raw feed heating value to gas heating value, and operability of each feed indicated the oak sawdust was the best feedstock.

  10. Imagens da prática profissional em academias de ginástica na cidade do Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Carlos Alberto de Andrade Coelho Filho

    2010-05-01

    Full Text Available O objetivo deste estudo é fornecer subsídios aos cursos de educação física, aproximando a formação profissional do campo de atuação em academias de ginástica. Através da análise de discurso, descrevemos e interpretamos a maneira como o profissional apresenta a si mesmo e a sua prática às outras pessoas, os meios pelos quais dirige e regula a impressão que formam a seu respeito e as atividades que pode ou não realizar. Os resultados confirmam que a prática do profissional de ginástica em academia vive momentos de tensão e impasse, o que implica a urgência de reformular a formação para essa prática nos cursos de graduação e de pós-graduação em educação física

  11. Battery condenser system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. In 2006, EPA finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This created an urgent need to collect additi...

  12. Mote cleaner system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. In 2006, EPA finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This created an urgent need to collect additi...

  13. First stage mote system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions using stack sampling. In 2006, the Environmental Protection Agency (EPA) finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This created an urgent n...

  14. Cyclone robber system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. In 2006, EPA finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This created an urgent need to collect additi...

  15. Master trash system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. In 2006, the Environmental Protection Agency (EPA) finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This cr...

  16. Environmental impact studies of Chrome rollers used by cotton roller ginning industries and design and development of pollution free chromeless RCF rollers.

    Science.gov (United States)

    Singh, Gurdeep; Iyer, G Vijayan

    2004-01-01

    This study focuses on environmental chromium contamination and pollution caused by the use of Chrome Composite Leather-Clad (CCLC) rollers commonly used in cotton roller ginning industries and attempts to eliminate this problem by providing eco-friendly chrome-less rollers.

  17. Gasification of oak sawdust, mesquite, corn stover and cotton gin trash in a countercurrent fluidized bed pilot reactor

    Energy Technology Data Exchange (ETDEWEB)

    Beck, S.R.; Wang, M.J.; Hightower, J.A.

    1981-01-01

    The Synthesis Gas From Manure (SGFM) process was designed to convert cattle feedlot manure to ammonia synthesis gas. Current work is aimed at using any biomass feedstock to produce either medium-Btu gas or chemical feedstocks. This paper presents a comparison of the experimental results compiled on gasification of oak sawdust, corn stover, mesquite, and cotton gin treash in the SGFM pilot plant. The SGFM process is based on a countercurrent, fluidized bed reactor. In this system, biomass is fed to the top of the reactor resulting in the fresh feed being partially dried by direct contact with hot product gas prior to entering the reaction zone. A weighted comparison of the product gas, hydrocarbon, and hydrogen yields, gas quality, calorific value of product gas, percentage conversion of raw feed heating value to gas heating value, and operability of each feed indicated that oak sawdust was the best feedstock. (Refs. 7).

  18. Evaluation of ligninolytic enzymes, ultrasonication and liquid hot water as pretreatments for bioethanol production from cotton gin trash.

    Science.gov (United States)

    Plácido, Jersson; Imam, Tahmina; Capareda, Sergio

    2013-07-01

    Cotton gin trash (CGT) is a ubiquitous cotton-production-waste resource which can be used for ethanol production. In this research, seven combinations of three pretreatments; ultrasonication, liquid hot water and ligninolytic enzymes were evaluated on CGT to select the best pretreatments combination that increased the cellulose conversion and the ethanol yield in the saccharification and fermentation processes, respectively. The structural changes in the cellulose, hemicellulose and lignin from CGT were followed using FT-IR after each pretreatment. All the pretreatment combinations modified the CGT's structure and composition compared with the unpretreated CGT, and the majority of them improved release of sugars originally present in the CGT. The best results were achieved by the sequential combination of ultrasonication, hot water, and ligninolytic enzymes with an improvement of 10% in the ethanol yield and cellulose conversion compared to the other pretreatments. These results are a contribution to develop a feasible bioethanol production from CGT. PMID:23665215

  19. Pressure Compensated Design of Ginned Cotton Tripper%皮棉卸料器的压力补偿设计

    Institute of Scientific and Technical Information of China (English)

    孙居彦

    2009-01-01

    通过理论分析和试验研究,给出了卸料器迎风面压力分布的一般分析方法,推导出了压力补偿计算公式,为设计改进卸料器结构、改善皮棉清理质量、提高机械性能提供了理论指导,具有重要的实际意义.%General analysis method of pressure distribution facing the wind in trippers was given by theoretical analysis and experimental study. At the same time the formula was inferred out to calculate pressure compensated. The study had an important significance that can provide theory guides for designing tripper structure, improve the quality of clearing ginned cotton, enhance mechanical performance of trippers and provide important practical significance.

  20. Conversion of cotton plant and cotton gin residues to fuels by the extruder-feeder liquefaction process

    Energy Technology Data Exchange (ETDEWEB)

    White, D.H. [Arizona Univ., Tucson, AZ (United States). Dept. of Chemical and Environmental Engineering; Coates, W.E. [Arizona Univ., Tucson, AZ (United States). Bioresources Research Facility; Wolf, D. [Ben-Gurion Univ. of the Negev, Beersheba (Israel). Dept. of Chemical Engineering

    1996-04-01

    Cotton is planted on a large scale in the USA, and is a major crop in Arizona. A large amount of cotton plant residue and cotton gin waste is produced annually, and these present a disposal problem. This material is comprised of cotton stalks, along with the upper portion of the taproot, and cotton gin trash. Cotton plant residues are a greater problem in the warmer regions of the USA as they serve as an over-wintering site for insect pests, and thus must be destroyed. Presently they are buried. Most field operations used to bury the residue are high energy consumers, and tend to destroy soil structure, thereby increasing the potential for erosion. This burial process is considered to be detrimental to the soil. One option to change the negative value of this biomass to a positive one is to harvest the cotton plants and combine them with other cotton wastes to produce a feedstock for liquefaction purposes. A process for harvesting and transporting the roots and stalks is being developed at the University of Arizona. In this paper we propose a combined harvesting/liquefaction system. This system would harvest and liquefy cotton wastes into oil and densified solid fuels with higher economic values. The extruder-feeder liquefaction process was developed at the University of Arizona in the 1980s under the sponsorship of the U.S. Department of Energy, with the goal to convert wood to a clean, 16000 btu/lb liquid fuel. The process has been redirected to other organic wastes using additional processing to yield ``value-added`` byproducts. Cotton wastes are good candidates for this process. By combining the harvesting and fuel processing operations, a negative value biomass would become a positive value biomass by (a) solving a disposal problem, and (b) producing high value fuels. (Author)

  1. Production cross sections and isomeric ratios for sup(110m)In/sup(110g)In formed in Cd (d,xn) reactions

    International Nuclear Information System (INIS)

    Excitation functions and isomeric cross sections ratios have been measured for the 110Cd (d,2n) and 111Cd (d,3n) reactions in which the isomeric pair sup(110m)In/sup(110g)In is produced. Activation method was employed and the irradiations were performed at the synchrocyclotron of the Comision Nacional de Energia Atomica, Argentina, with an incident energy of 27.MeV. (author)

  2. Effects of Pyrolysis Temperature on Product Yields and Energy Recovery from Co-Feeding of Cotton Gin Trash, Cow Manure, and Microalgae: A Simulation Study

    OpenAIRE

    Hanif, Muhammad Usman; Capareda, Sergio C.; Iqbal, Hamid; Arazo, Renato Ortiz; Baig, Muhammad Anwar

    2016-01-01

    The intensive search of new and cleaner energy catches interest in recent years due to huge consumption of fossil fuels coupled with the challenge of energy and environmental sustainability. Production of renewable and environmentally benign energy from locally available raw materials is coming in the frontline. In this work, conversion of the combined biomass (cotton gin trash, cow manure, and Microalgae [Nannochloropsis oculata]) through batch pyrolysis has been investigated. The effect of ...

  3. Educação física/ginástica : uma associação com raízes no tempo

    OpenAIRE

    Faustino, António

    1995-01-01

    Procede-se proceder ao enquadramento da Educação Física no Sistema Educativo português. Para o efeito através duma consulta bibliográfica a diferentes fontes são indicados alguns factos que contribuiram para a associação entre Educação Física e Ginástica, enquanto disciplina escolar, a qual tem perdurado no tempo.

  4. Modelling and Predicting the Breaking Strength and Mass Irregularity of Cotton Rotor-Spun Yarns Containing Cotton Fiber Recovered from Ginning Process by Using Artificial Neural Network Algorithm

    Directory of Open Access Journals (Sweden)

    Mohsen Shanbeh

    2011-01-01

    Full Text Available One of the main methods to reduce the production costs is waste recycling which is the most important challenge for the future. Cotton wastes collected from ginning process have desirable properties which could be used during spinning process. The purpose of this study was to develop predictive models of breaking strength and mass irregularity (CV% of cotton waste rotor-spun yarns containing cotton waste collected from ginning process by using the artificial neural network trained with backpropagation algorithm. Artificial neural network models have been developed based on rotor diameter, rotor speed, navel type, opener roller speed, ginning waste proportion and yarn linear density as input parameters. The parameters of artificial neural network model, namely, learning, and momentum rate, number of hidden layers and number of hidden processing elements (neurons were optimized to get the best predictive models. The findings showed that the breaking strength and mass irregularity of rotor spun yarns could be predicted satisfactorily by artificial neural network. The maximum error in predicting the breaking strength and mass irregularity of testing data was 8.34% and 6.65%, respectively.

  5. A propósito de Ling, da ginástica sueca e da circulação de impressos em língua portuguesa

    Directory of Open Access Journals (Sweden)

    Moreno Andrea

    2015-06-01

    Full Text Available Este artigo analisa o sistema sueco de ginástica criado por P. H. Ling e retoma a trajetória de seu precursor e a vocação do Instituto Central de Ginástica de Estocolmo no papel de preservação e divulgação dos preceitos lingianos. Especificamente, atentamo-nos para a vulgarização e a circulação do método por meio de manuais em língua portuguesa produzidos entre fins do século XIX e início do XX. Tem como fontes os manuais escolares escritos por Pedro Borges, Antonio M. Ferreira, Paulo Lauret e Arthur Higgins. Percebemos neles forte inspiração na ginástica sueca, revelada nas lições sistematizadas e na valorização de aspectos como retidão, racionalidade, simetria, correção, educação da vontade e aperfeiçoamento físico.

  6. 京族民间文艺与自然生态%The Gin Folk Literatures & Arts and Nature & Ecology

    Institute of Scientific and Technical Information of China (English)

    陈丽琴

    2012-01-01

    京族民间文艺的生成、发展与自然生态环境之间有着密切关系。京族民间文艺作品体现了人们独特的生态意识,自然环境对京族民间口承文艺、民间舞蹈、服饰艺术以及民间工艺的产生与传承具有深刻的影响,造就了京族民间文艺的独特风格,没有京族地区独特的生态环境,其民间文艺也就失去所赖以产生的土壤。%The Gin folk literatures and arts linked closely with the natural and ecological environment in both its forming and the development.They reveals the unique ecologically awareness of the Gin people,and was deeply ingrained by the environment in the forming and passing down of the oral fork literatures,dances,dresses and handicrafts.Without the unique ecological environment,the Gin folk literatures and arts would lose its foundation.

  7. X-ray repair cross-complementing gene 1 Arg399GIn polymorphism and glioma risk among Asians A meta-analysis based on 2326 cases and 3610 controls

    Institute of Scientific and Technical Information of China (English)

    Liang Zhang; Zhiqun Qiu; Jiaohua Luo; Weiqun Shu

    2012-01-01

    OBJECTIVE:Previous reports have demonstrated that X-ray repair cross-complementing gene 1 (XRCC1) Arg399GIn polymorphism is a possible risk factor for several cancers.Published data on the association of XRCC1Arg399GIn polymorphism with glioma susceptibility have generated conflicting results.This study is designed to precisely estimate the relationship.DATA RETRIEVAL:A computer-based online retrieval of Medline,EMBASE,OVID,Sciencedirect,and Chinese National Knowledge Infrastructure was performed to search papers regarding association of XRCC1 Arg399GIn polymorphisms with glioma published up to April 2012.SELECTION CRITERIA:Two investigators selected data independently.Meta analysis was then performed for the selected studies using STATA 11.0 software after strict selection.Heterogeneity test,sensitivity analysis and publication bias assessments were then conducted.MAIN OUTCOME MEASURES:Association of XRCC1 Arg399GIn polymorphism with glioma risk.RESULTS:A total of nine case-controlled studies comprising 2 326 cases and 3 610 controls were selected for final analysis.The overall data failed to indicate a significant association of XRCC1 Arg399GIn polymorphism with glioma risk (GIn/GIn vs.Arg/Arg:odds ratio (OR) =1.11; 95% confidence interval (Cl) =0.94-1.31; dominant model:OR =1.06; 95%Cl=0.95-1.18; recessive model:OR =1.04; 95%Cl=0.81-1.34).However,subgroup analysis regarding ethnicity showed an increased risk among Asians (GIn/GIn vs.Arg/Arg:OR =1.70; 95%Cl=1.17-2.46; dominant model:OR =1.40; 95%Cl=1.10-1.78; recessive model:OR =1.46; 95%Cl =1.04-2.05) but not Caucasians or mixed ethnicities.CONCLUSION:XRCC1 Arg399GIn polymorphism might modify the susceptibility to glioma among Asians but not Caucasians.Further large and well-designed studies are needed to confirm this conclusion.

  8. Efeito do treinamento proprioceptivo no equilíbrio de atletas de ginástica rítmica

    Directory of Open Access Journals (Sweden)

    Marianne Lamb

    2014-10-01

    Full Text Available INTRODUÇÃO: A ginástica rítmica (GR é uma modalidade esportiva que combina arte a gestos biomecânicos de alta complexidade, que requer alto nível de desenvolvimento de qualidades físicas como: agilidade, flexibilidade, força, impulsão e destreza. Nessa modalidade, estudos comprovam que as lesões mais frequentes ocorrem nas articulações do joelho e tornozelo e, em alguns casos, nas extremidades do punho, sendo os entorses as mais registradas, principalmente em atletas de nível de competição. Diante disso, a propriocepção e o controle muscular possuem papel fundamental na estabilidade articular dinâmica, já que após lesões ortopédicas algumas características sensório-motoras são alteradas e devem ser o foco de programas de reabilitação para que se obtenha melhor retorno às atividades como antes da lesão. OBJETIVO: Avaliar o efeito do treinamento proprioceptivo sobre o equilíbrio postural de atletas de ginástica rítmica, a partir da utilização da plataforma de força. MÉTODOS: Foi realizado um treinamento proprioceptivo no período de 2 meses, subdividido em três fases, com progressão da complexidade dos exercícios a cada fase de treinamento. RESULTADOS: Podemos observar que houve uma melhora significativa dos resultados da última fase do treinamento, quando foram utilizados exercícios de maior complexidade e agilidade, de acordo com o protocolo. CONCLUSÃO: Por se tratarem de atletas de GR de alto desempenho e apresentarem predomínio de atividades em apoio unipodal, os protocolos de propriocepção devem ser mais intensos, gerando um maior nível de perturbações e desequilíbrios posturais.

  9. Ethanol production of semi-simultaneous saccharification and fermentation from mixture of cotton gin waste and recycled paper sludge.

    Science.gov (United States)

    Shen, Jiacheng; Agblevor, Foster A

    2011-01-01

    Ethanol production from the steam-exploded mixture of 75% cotton gin waste and 25% recycled paper sludge in various conditions was investigated by semi-simultaneous saccharification and fermentation (SSSF) consisting of a pre-hydrolysis and a simultaneous saccharification and fermentation (SSF). Four cases were studied: 24-h pre-hydrolysis + 48-h SSF (SSSF 24), 12-h pre-hydrolysis + 60-h SSF (SSSF 12), 72-h SSF, and 48-h hydrolysis + 24-h fermentation (SHF). The ethanol concentration, yield, and productivity of SSSF 24 were higher than those of the other operations. A model of SSF was used to simulate the data for four components in SSF. The analysis of the reaction rates of cellobiose, glucose, cell, and ethanol using the model and the parameters from the experiments showed that there was a transition point of the rate-controlling step at which the cell growth control in the initial 2 h was changed to the cellobiose reaction control in later period during ethanol production of SSF from the mixture. PMID:20559849

  10. Activated carbons from flax shive and cotton gin waste as environmental adsorbents for the chlorinated hydrocarbon trichloroethylene.

    Science.gov (United States)

    Klasson, K Thomas; Wartelle, Lynda H; Lima, Isabel M; Marshall, Wayne E; Akin, Danny E

    2009-11-01

    Agricultural by-products represent a considerable quantity of harvested commodity crops. The use of by-products as precursors for the production of widely used adsorbents, such as activated carbons, may impart a value-added component of the overall biomass harvested. Our objective in this paper is to show that flax shive and cotton gin waste can serve as a precursor for activated carbon that can be used for adsorption of trichloroethylene (TCE) from both the liquid and gas phases. Testing was conducted on carbon activated with phosphoric acid or steam. The results show that activated carbon made from flax shive performed better than select commercial activated carbons, especially at higher TCE concentrations. The activation method employed had little effect on TCE adsorption in gas or vapor phase studies but liquid phase studies suggested that steam activation is slightly better than phosphoric acid activation. As expected, the capacity for the activated carbons depended on the fluid phase equilibrium concentration. At a fluid concentration of 2 mg of TCE/L of fluid, the capacity of the steam activated carbon made from flax shive was similar at 64 and 80 mg TCE/g of carbon for the vapor and liquid phases, respectively. Preliminary cost estimates suggest that the production costs of such carbons are $1.50 to $8.90 per kg, depending on activation method and precursor material; steam activation was significantly less expensive than phosphoric acid activation. PMID:19540755

  11. Ginástica artística competitiva e a filosofia dos técnicos Competitive artistic gymnastics and coaches' philosophy

    OpenAIRE

    Myrian Nunomura; Mauricio Santos Oliveira; Odilon José Roble; Michele Carbinatto

    2012-01-01

    O presente estudo é um recorte do projeto original intitulado "Diagnóstico do Processo de Formação Esportiva da Ginástica Artística no Brasil". Um dos temas de análise foi a filosofia de trabalho dos técnicos que atuam nas categorias de base e preparam os potenciais ginastas para as seleções nacionais. Entrevistamos 46 técnicos de 29 instituições esportivas do Brasil. Para a coleta dos dados utilizamos a entrevista semiestruturada e, para o tratamento dos dados, a análise de conteúdo proposta...

  12. Prediction of Ungauged River Basin for Hydro Power Potential and Flood Risk Mitigation; a Case Study at Gin River, Sri Lanka

    Science.gov (United States)

    Ratnayake, A. S.

    2011-12-01

    The most of the primary civilizations of the world emerged in or near river valleys or floodplains. The river channels and floodplains are single hydrologic and geomorphic system. The failure to appreciate the integral connection between floodplains and channel underlies many socioeconomic and environmental problems in river management today. However it is a difficult task of collecting reliable field hydrological data. Under such situations either synthetic or statistically generated data were used for hydraulic engineering designing and flood modeling. The fundamentals of precipitation-runoff relationship through synthetic unit hydrograph for Gin River basin were prepared using the method of the Flood Studies Report of the National Environmental Research Council, United Kingdom (1975). The Triangular Irregular Network model was constructed using Geographic Information System (GIS) to determine hazard prone zones. The 1:10,000 and 1:50,000 topography maps and field excursions were also used for initial site selection of mini-hydro power units and determine flooding area. The turbines output power generations were calculated using the parameters of net head and efficiency of turbine. The peak discharge achieves within 4.74 hours from the onset of the rainstorm and 11.95 hours time takes to reach its normal discharge conditions of Gin River basin. Stream frequency of Gin River is 4.56 (Junctions/ km2) while the channel slope is 7.90 (m/km). The regional coefficient on the catchment is 0.00296. Higher stream frequency and gentle channel slope were recognized as the flood triggering factors of Gin River basin and other parameters such as basins catchment area, main stream length, standard average annual rainfall and soil do not show any significant variations with other catchments of Sri Lanka. The flood management process, including control of flood disaster, prepared for a flood, and minimize it impacts are complicated in human population encroached and modified

  13. A medida da gordura: O interno e o íntimo na academia de ginástica

    Directory of Open Access Journals (Sweden)

    Carlos Emanuel Sautchuk

    2007-04-01

    Full Text Available A partir de pesquisa etnográfica e bibliográfica, o presente artigo trata da gordura nas academias de ginástica, enfocando questões relativas ao corpo e à pessoa. A análise da avaliação corporal evidencia os termos de uma produção científica que elabora o corpo como um metabolismo caracterizado pelo risco e pela maleabilidade. São os conteúdos corporais que estão em jogo, de modo que o ideal estético não é a magreza, mas a "definição" do corpo, fruto da sobreposição da gordura pelo músculo. A busca da saúde caracteriza-se pela gerência constante do risco inerente ao balanço destes componentes. Beleza e saúde são formas de cuidar de si - imperativo caro ao individualismo contemporâneo - através do manejo dos conteúdos corporais. Assim, a avaliação corporal delineia os elementos internos e os aspectos da intimidade, e dota as práticas corporais da capacidade de empreender um engajamento entre eles.Based on both ethnographic and bibliographic research, the present article examines the subject of fat in gymnastic academies, focusing on issues relating to the body and the person. An analysis of body evaluation evinces the terms of a scientific production that configures the body as a metabolism subject to risk and malleability. What is at stake are body contents, where the aesthetic ideal is not thinness, but the ‘toning’ of the body, the result of replacing fat with muscle mass. The search for beauty involves constant management of the risk inherent in balancing these components. Beauty and health are forms of self-care - an imperative central to contemporary individualism - through the management of body contents. Thus, body evaluation delineates internal elements and aspects of intimacy, and endows bodily practices with the capacity to stimulate an interaction between them.

  14. Second-stage mote system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This report is part of a project to characterize cotton gin emissions from the standpoint of stack sampling. In 2006, the Environmental Protection Agencey (EPA) finalized and published a more stringent standard for particulate matter with nominal diameter less than or equal to 2.5 µm (PM2.5). This c...

  15. Third stage seed-cotton cleaning system PM2.5 emission factors and rates for cotton gins: Method 201A combination PM10 and PM2.5 sizing cyclones

    Science.gov (United States)

    This manuscript is part of a series of manuscripts that detail a project to characterize cotton gin emissions from the standpoint of stack and ambient sampling. The impetus behind the project was the 2006 EPA implementation of a more stringent standard for particulate matter less than or equal to 2....

  16. Teste GIN: detecção de gap em crianças com desvio fonológico Noise test: gap detection in children with phonological deviation

    Directory of Open Access Journals (Sweden)

    Elaine Feltre Assis

    2013-02-01

    Full Text Available OBJETIVO: investigar a resolução temporal: detecção de gaps em crianças com desvios fonológicos por meio do teste GIN e relacionar o grau do desvio fonológico com desempenho no teste GIN. MÉTODO: 6 indivíduos, de ambos os gêneros, 5 meninos e 1 menina, com idade entre 10 e 11 anos, com diagnóstico de desvio fonológico, em atendimento na clínica-escola do curso de Fonoaudiologia da FEAD de Belo Horizonte/MG, todos com ausência de perda auditiva e problemas neurológicos e/ou cognitivos. Os participantes foram submetidos ao Teste GIN, em intensidade de 50dB acima do limiar. RESULTADOS: das 06 crianças avaliadas, 5 (83,33% encontram-se alteradas e apenas 1 (16,67% obteve valores dentro do padrão de normalidade. Apesar da pequena amostra, viu-se que 83,33% das crianças com desvio fonológico tiveram limiares do GIN aquém do esperado para faixa etária. Porém não foi possível estabelecer uma relação direta entre grau de classificação do desvio fonológico e o baixo desempenho obtido no teste GIN, no qual apenas 1 criança com desvio médio moderado apresentou pior desempenho no teste GIN. CONCLUSÃO: crianças com desvio fonológico podem apresentar alteração no processamento temporal.PURPOSE: to investigate the temporal resolution, as for: gaps detection in children with phonological deviation through noise test and related with the degree of phonological performance in noise test. METHOD: 6 patients of both genders, five boys and one girl, aged between 10 and 11 year-old with phonological disorder' diagnosis in attendance at the school clinic of the Speech Therapy course (FEAD Belo Horizonte / MG, all with no hearing loss and no neurological and / or cognitive problems. The subjects underwent the GIN test at intensity of 50dB above the threshold. RESULTS: from the 6 evaluated children, 5 (83.33% had abnormal responses at gin test and only one (16.67% had values within the normal range. Despite the small sample, it was

  17. Avaliação da qualidade de vida em funcionários praticantes de ginástica holística em empresa

    OpenAIRE

    Fernanda Fonseca dos Santos Lopes; Zelia Zilda Lourenço de Camargo Bittencourt

    2012-01-01

    Introdução: A qualidade de vida na empresa é um importante recurso de promoção de saúde, e pode ser desenvolvida a partir da pratica de atividades físicas. O exercício físico é uma forma de lazer e de restaurar a saúde dos efeitos da rotina estressante do trabalho, proporcionando condições de melhor desempenho e produtividade. A Ginástica Holística atua através da sensibilização e da tomada de consciência, de modo a levar o sujeito a uma melhor utilização do seu potencial perceptivo, sensoria...

  18. Interação entre leptina, ginástica artística, puberdade e exercício em atletas do sexo feminino

    OpenAIRE

    Tatiana de Barros Meira; Myrian Nunomura

    2010-01-01

    Há evidências de que as atletas de ginástica artística (GA) apresentam desregulações no sistema reprodutório em relação à média populacional. A interação entre alguns fatores pode explicar esse fato: genética, estresse do exercício, baixa porcentagem de gordura corporal, má nutrição e ação da leptina. Por meio de uma revisão de literatura, discutimos resultados de pesquisas que relacionam a ação do hormônio leptina e desregulações no sistema reprodutório durante a puberdade em atletas de GA d...

  19. Leyendo el sur. A propósito del libro Cantes al Amorsillega de Ginés Liébana

    Directory of Open Access Journals (Sweden)

    Bartolomé Delgado Cerrillo

    2011-06-01

    Full Text Available A través de los poemas del libro Cantes al Amorsillega de Ginés Liébana, haremos un recorrido por las principales coplas del cante flamenco, incidiendo en la personal interpretación que nos da el autor y aportando su relación con la significación de las formas poéticas y las imágenes con que las ilustra. Este libro es valioso para ser integrado en la educación reglada como producto cultural. Y es valioso no solo como portador de contenidos antropológicos, sociológicos, coreográficos, musicales, poéticos e históricos, sino también como compendio de los estereotipos culturales que caracterizan la identidad cultural andaluza.

  20. Avaliação nutricional e antropométrica de praticantes de exercício físico em ginásio

    OpenAIRE

    Dias, Rita Margarida Rodrigues

    2014-01-01

    A preocupação acrescida com a condição e aparência física, refletem-se em cuidados com o estado de saúde e bem-estar e são, nos últimos anos, responsáveis pela expansão dos ginásios e health clubs.Mas, atividade física e a alimentação estão intimamente ligadas, tanto no que respeita à estética como também, com maiores preocupações, no que respeita à saúde. Daí que as duas vertentes estejam ligadas à imagem de pessoas saudáveis e vigorosas. Na verdade, a capacidade de rendimento do organism...

  1. Relationship between external stink bug (Hemiptera: Pentatomidae) boll-feeding symptoms and internal boll damage with respect to cotton lint gin-out and fiber quality.

    Science.gov (United States)

    Blinka, Eric L; Herbert, Ames; Malone, Sean; Van Duyn, John W; Roberts, Phillip; Bradley, J R; Bacheler, Jack S

    2010-12-01

    Cotton, Gossypium hirsutum L., bolls from 17 field locations in northeastern North Carolina and southeastern Virginia, having 20% or greater internal boll damage, were studied to determine the relationship between external feeding symptoms and internal damage caused by stink bug (Hemiptera: Pentatomidae) feeding. In 2006 and 2007, two cohorts of 100 bolls each were sampled at all field locations. The first cohort was removed as bolls reached approximately quarter size in diameter (2.4 cm). External and internal symptoms of stink bug feeding were assessed and tabulated. Concurrent to when the first cohort was collected, a second cohort of quarter-size-diameter bolls was identified, tagged, examined in situ for external feeding symptoms (sunken lesions), and harvested at the black seed coat stage. Harvested bolls were assessed for internal damage and locks were categorized (undamaged, minor damage, or major damage), dried, and ginned. Lint samples from each damage category were submitted for high volume instrument and advanced fiber information system quality analyses. Significant, moderately strong Pearson correlation coefficients existed between number of external stink bug feeding lesions and internal damage. Pearson correlation of total external lesions with total internal damage was stronger than any correlation among the other single components compared. Predictability plots indicated a rapid increase in relationship strength when relating external stink bug lesions to internal damage as the number of external lesions increased. Approximately 90% predictability of internal damage was achieved with four (2006) or six (2007) external lesions per boll. Gin-turnout and fiber quality decreased with increasing intensity of internal stink bug damage. PMID:21309249

  2. Ginástica geral na escola: uma proposta metodológica General gymnastics at school: A methodological propositon Gimnasia general en la escuela: Una propuesta metodológica

    Directory of Open Access Journals (Sweden)

    2006-11-01

    Full Text Available Este trabalho tem por objetivo discutir a Ginástica como conteúdo da Educação Física escolar, por meio da Ginástica geral como proposta metodológica. Tal proposta tem como perspectiva a integração das diversas manifestações gímnicas e os outros componentes da cultura corporal, sendo sua principal característica a ausência da competição. PALAVRAS-CHAVE: ginástica – ginástica geral – educação física escolar. This study aims at discussing gym classes as a part of school physical education, using General Gymnastics as a methodological approach. Such an approach has the perspective of integrating the several gymnastic manifestations to the other components of body culture, with the lack of competition as its main feature. KEY WORDS: gym – general gymnastics – physical education in schools. Este trabajo tiene por objetivo discutir la Gimnasia como contenido de la Educación Física escolar, por medio de la Gimnasia general como propuesta metodológica. Tal propuesta tiene como perspectiva la integración de diversas manifestaciones gímnicas y los otros componentes de la cultura corporal, siendo su principal característica la ausencia de la competencia. PALABRAS-CLAVE: gimnasia – gimnasia general – educación física escolar.

  3. Comparação do tempo de reação entre atletas de Basquetebol, Ginástica Artística e não atletas

    Directory of Open Access Journals (Sweden)

    Alessandro Teodoro Bruzi

    2013-06-01

    Full Text Available O propósito deste estudo foi comparar o tempo de reação entre atletas de Basquetebol (grupo Basquetebol, Ginástica Artística (grupo Ginástica e não atletas (grupo controle. Os indivíduos foram avaliados no tempo de reação simples e de escolha através do instrumento MULTPSY 821 e Sistema de Testes de Viena, respectivamente. Os resultados indicaram somente diferença significativa entre os grupos na medida de tempo de reação simples. O grupo controle apresentou maior tempo de reação comparado aos outros grupos. Esses achados indicam que a prática de Basquetebol e Ginástica Artística pode melhorar o tempo de reação simples, que é uma capacidade perceptiva necessária em atividades diárias.

  4. A recepção da ginástica sueca em Portugal nas primeiras décadas do século XX: conformidades e dissensões culturais e políticas

    Directory of Open Access Journals (Sweden)

    Luís Miguel Carvalho

    2015-06-01

    Full Text Available O artigo aborda a presença persistente da "ginástica sueca" como referente principal da educação física em contexto escolar em Portugal, nas primeiras décadas do século XX, e as recepções distintas de que foi alvo. Numa primeira parte, o texto centra-se na compreensão da centralidade adquirida pela "ginástica sueca", em múltiplos contextos geopolíticos, no contexto da consolidação do projeto da escola de massas. Numa segunda parte, o texto documenta e analisa algumas deslocações interpretativas ocorridas na recepção da "ginástica sueca" em Portugal, entre 1920 e 1940, bem como os realinhamentos educativos, sociais e políticos que acompanharam esses conflitos sobre a sua "correta" mobilização para o contexto escolar português.

  5. El Manuscrito Cerramientos y trazas de montea de Ginés Martínez de Aranda

    Directory of Open Access Journals (Sweden)

    Calvo López, José

    2009-03-01

    Full Text Available Ginés Martínez de Aranda, master mason in the cathedrals of Cádiz and Santiago de Compostela during the late 16th century, wrote a manuscript, Cerramientos y trazas de montea, concerning geometrical problems in stone construction. This article deals with the copy of Cerramientos in the Servicio Histórico Militar (Madrid, its copyist, dating, the existence of an earlier version and whether or not Aranda intended to publish it. Also examined are the connections between Cerramientos and other Spanish stonecutting texts of the period, such as those by Alonso de Vandelvira, Cristóbal de Rojas, Alonso de Guardia or Juan de Portor y Castro. Finally, an assessment is made of Martínez de Aranda’s original contributions to European stonecutting literatura.

    El arquitecto y cantero baezano Ginés Martínez de Aranda, que trabajó a finales del siglo XVI y ejerció la maestría de las catedrales de Cádiz y Santiago de Compostela, compuso un manuscrito llamado Cerramientos y trazas de montea, que trata de los problemas geométricos de la construcción pétrea. El artículo analiza la copia del manuscrito conservada en el Servicio Histórico Militar de Madrid, la identidad del copista, la fecha de la primera redacción de la obra y la de la copia conservada, y el destino de la obra; a continuación, se estudia la relación del manuscrito con otras obras españolas de cantería, en especial las de Alonso de Vandelvira, Cristóbal de Rojas, Alonso de Guardia y Juan de Portor y Castro, para finalizar tratando diversas aportaciones originales de Aranda a la literatura europea de la cantería.

  6. Effects of Pyrolysis Temperature on Product Yields and Energy Recovery from Co-Feeding of Cotton Gin Trash, Cow Manure, and Microalgae: A Simulation Study.

    Science.gov (United States)

    Hanif, Muhammad Usman; Capareda, Sergio C; Iqbal, Hamid; Arazo, Renato Ortiz; Baig, Muhammad Anwar

    2016-01-01

    The intensive search of new and cleaner energy catches interest in recent years due to huge consumption of fossil fuels coupled with the challenge of energy and environmental sustainability. Production of renewable and environmentally benign energy from locally available raw materials is coming in the frontline. In this work, conversion of the combined biomass (cotton gin trash, cow manure, and Microalgae [Nannochloropsis oculata]) through batch pyrolysis has been investigated. The effect of temperature to the production of energy fuels such as bio-oil, char, and biogas have been simulated considering the yield and energy content as responses. Result of the investigation generally revealed that the proportions of the different biomass did not significantly affect the product yield and energy recovery. Significant effect of temperature is evident in the simulation result of energy recovery whereby maximum conversion was achieved at 400°C for char (91 wt%), 600°C for syngas (22 wt%), and 551°C for bio-oil (48 wt%). Overall energy conversion efficiency of 75.5% was obtained at 589°C in which 15.6 MJ/kg of mixed biomass will be elevated to pyrolysis products.

  7. Effects of Pyrolysis Temperature on Product Yields and Energy Recovery from Co-Feeding of Cotton Gin Trash, Cow Manure, and Microalgae: A Simulation Study.

    Directory of Open Access Journals (Sweden)

    Muhammad Usman Hanif

    Full Text Available The intensive search of new and cleaner energy catches interest in recent years due to huge consumption of fossil fuels coupled with the challenge of energy and environmental sustainability. Production of renewable and environmentally benign energy from locally available raw materials is coming in the frontline. In this work, conversion of the combined biomass (cotton gin trash, cow manure, and Microalgae [Nannochloropsis oculata] through batch pyrolysis has been investigated. The effect of temperature to the production of energy fuels such as bio-oil, char, and biogas have been simulated considering the yield and energy content as responses. Result of the investigation generally revealed that the proportions of the different biomass did not significantly affect the product yield and energy recovery. Significant effect of temperature is evident in the simulation result of energy recovery whereby maximum conversion was achieved at 400°C for char (91 wt%, 600°C for syngas (22 wt%, and 551°C for bio-oil (48 wt%. Overall energy conversion efficiency of 75.5% was obtained at 589°C in which 15.6 MJ/kg of mixed biomass will be elevated to pyrolysis products.

  8. Efeitos da ginástica laboral na dor nas costas e nos hábitos posturais adotados no ambiente de trabalho

    Directory of Open Access Journals (Sweden)

    Cláudia Tarragô Candotti

    2011-09-01

    Full Text Available O objetivo deste estudo foi verificar o efeito da Ginástica Laboral (GL sobre a dor nas costas e sobre os hábitos posturais de trabalhadores que ficam por longos períodos na posição sentada. Participaram 30 trabalhadores do setor administrativo, divididos em grupo controle (n=15 e grupo experimental (n=15, sendo este submetido a sessões de GL durante três meses. Ambos os grupos foram avaliados por um questionário de dor e postura, sendo as respostas codificadas, tabuladas e submetidas ao teste de Wilcoxon para verificar as diferenças entre pré e pós-experimento (α=0,05. Os resultados demonstraram que a GL proporcionou diminuição da intensidade e frequência da dor referida aos trabalhadores do grupo experimental, e mudança do hábito postural durante o trabalho, melhorando a postura sentada.

  9. Interação entre leptina, ginástica artística, puberdade e exercício em atletas do sexo feminino

    Directory of Open Access Journals (Sweden)

    Tatiana de Barros Meira

    2010-09-01

    Full Text Available Há evidências de que as atletas de ginástica artística (GA apresentam desregulações no sistema reprodutório em relação à média populacional. A interação entre alguns fatores pode explicar esse fato: genética, estresse do exercício, baixa porcentagem de gordura corporal, má nutrição e ação da leptina. Por meio de uma revisão de literatura, discutimos resultados de pesquisas que relacionam a ação do hormônio leptina e desregulações no sistema reprodutório durante a puberdade em atletas de GA do sexo feminino. Podemos afirmar que existe uma interação entre a seleção natural na modalidade, a baixa ingestão calórica e o estresse do treinamento, que ocasionaria a diminuição de leptina plasmática e causaria desregulações no sistema reprodutório durante a puberdade.

  10. Epifisiólise proximal do úmero em atleta de ginástica olímpica Proximal humeral epiphysiolysis in a gymnast

    Directory of Open Access Journals (Sweden)

    Benno Ejnisman

    2007-01-01

    Full Text Available CONTEXTO: Existem diversas causas de dor no ombro do atleta. Estruturas como tendões e ligamentos são freqüentemente acometidas por lesões. No atleta jovem com esqueleto imaturo, as afecções ósseas devem ser lembradas como possíveis causas de dor. RELATO DE CASO: Os autores relatam a ocorrência da epifisiólise proximal do úmero em um atleta de ginástica olímpica e discutem a literatura a respeito do assunto. CONCLUSÃO: A lesão da placa fisária proximal do úmero em atletas é pouco freqüente. O diagnóstico precoce e correto tratamento evitam que ocorra deformidades severas e déficits funcionais.CONTEXT: There are different causes for shoulder pain in athletes. Structures such as tendons and ligaments are frequently affected by injuries. In young athletes with immature skull, bone injuries must be ruled as a cause of pain. CASE REPORT: The authors present a case of proximal humeral epiphysiolysis in a gymnast and review literature addressing this topic. CONCLUSION: Injuries on proximal humeral physeal plates of athletes are uncommon. The early diagnosis and correct treatment can avoid deformity and functional deficits.

  11. Effects of Pyrolysis Temperature on Product Yields and Energy Recovery from Co-Feeding of Cotton Gin Trash, Cow Manure, and Microalgae: A Simulation Study.

    Science.gov (United States)

    Hanif, Muhammad Usman; Capareda, Sergio C; Iqbal, Hamid; Arazo, Renato Ortiz; Baig, Muhammad Anwar

    2016-01-01

    The intensive search of new and cleaner energy catches interest in recent years due to huge consumption of fossil fuels coupled with the challenge of energy and environmental sustainability. Production of renewable and environmentally benign energy from locally available raw materials is coming in the frontline. In this work, conversion of the combined biomass (cotton gin trash, cow manure, and Microalgae [Nannochloropsis oculata]) through batch pyrolysis has been investigated. The effect of temperature to the production of energy fuels such as bio-oil, char, and biogas have been simulated considering the yield and energy content as responses. Result of the investigation generally revealed that the proportions of the different biomass did not significantly affect the product yield and energy recovery. Significant effect of temperature is evident in the simulation result of energy recovery whereby maximum conversion was achieved at 400°C for char (91 wt%), 600°C for syngas (22 wt%), and 551°C for bio-oil (48 wt%). Overall energy conversion efficiency of 75.5% was obtained at 589°C in which 15.6 MJ/kg of mixed biomass will be elevated to pyrolysis products. PMID:27043929

  12. Desequilíbrio hormonal e disfunção menstrual em atletas de ginástica rítmica

    Directory of Open Access Journals (Sweden)

    Sabrina Macedo Hott Coelho

    2015-09-01

    Full Text Available ResumoObjetivou-se avaliar a magnitude das alterações de hormônios reguladores do ciclo menstrual (CM e testosterona de atletas de ginástica rítmica (GR de alto rendimento, em período pré-competitivo para a Olimpíada de Beijing. Foram realizadas coletas de sangue em 4 momentos, com intervalos de 7 dias. Os hormônios estrogênio, progesterona, luteinizante (LH, folículo estimulante (FSH e testosterona foram mensurados por quimioluminescência. As atletas (n = 7 relataram disfunção menstrual e houve expressiva variação hormonal intra e entre indivíduos. Das amostras de estrogênio, 65% ficaram abaixo do valor referencial clínico, enquanto que as concentrações de progesterona e LH ficaram próximas ao limite inferior. Para FSH e testosterona, 10% e 30% das amostras, respectivamente, ficaram abaixo do referencial. Conclui-se que atletas de alto rendimento em GR apresentam maior risco de desequilíbrio hormonal e disfunção menstrual, o que inspira necessidade de atenção clínica.

  13. Ginástica artística competitiva e a filosofia dos técnicos Competitive artistic gymnastics and coaches' philosophy

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    Myrian Nunomura

    2012-12-01

    Full Text Available O presente estudo é um recorte do projeto original intitulado "Diagnóstico do Processo de Formação Esportiva da Ginástica Artística no Brasil". Um dos temas de análise foi a filosofia de trabalho dos técnicos que atuam nas categorias de base e preparam os potenciais ginastas para as seleções nacionais. Entrevistamos 46 técnicos de 29 instituições esportivas do Brasil. Para a coleta dos dados utilizamos a entrevista semiestruturada e, para o tratamento dos dados, a análise de conteúdo proposta por Bardin (2010. Constatamos que a base filosófica ainda é inconsistente e que, em muitas instituições, esta se confunde com os próprios objetivos do programa de treinamento ou simplesmente inexiste. O fato merece considerações e criticas, mas sabemos que a filosofia não é desenvolvida durante os cursos de formação dos técnicos.The current study is a piece from the original project entitled "Diagnosis of the Artistic Gymnastics Development Program in Brazil". Among others issues, the coaching philosophy of coaches who are developing gymnasts that may be representative of the national team was a matter of analysis. We interviewed 46 coaches from 29 sports institutions in Brazil. As data collection we used a semi-structured interview and for data treatment we adopted the content analysis method of Bardin (2010. We have found out that there is an inconsistent philosophical basis, and in many institutions this has been mixed up with the objectives of the training program or just doesn't exist. This fact needs consideration and criticism, but we know that the coaching philosophy is not developed during the coaching education courses.

  14. Influência do tipo de colheita e de beneficiamento, na qualidade da fibra e do fio de algodão Effects of harvesting practices and gin machinery on the technological properties of cotton fibers and yarn

    Directory of Open Access Journals (Sweden)

    Carlos A. M. Ferraz

    1977-01-01

    Full Text Available São relatados e discutidos os efeitos de colheita manual e mecânica na qualidade da fibra e do fio do algodão e a influência do beneficiamento em máquinas descaroçadoras de rolo e de serras, sendo esta última equipada para pré e pós-limpeza do algodão, especial para beneficiamento de algodão colhido mecanicamente. Os ensaios foram conduzidos no ano de 1970/71, em Araras e Paranapanema. A colheita mecânica não afetou a qualidade da fibra ou do fio, sendo inclusive superior à manual para comprimento de fibra, em Paranapanema, e para micronaire, em Araras. O beneficiamento em máquinas descaroçadoras de serras prejudicou sempre a qualidade da fibra e do fio. A característica de resistência da fibra medida pelo aparelho Pressley não foi afetada.Effects of harvesting practices and gin machinery on the properties of cotton fibers and yarn were studied for three commercial varieties: IAC 13-1, IAC RM3, and IAC RM4 SM5. There was no difference in the majority of technological properties, between hand-picked cotton and machine-picked cotton. However, machine-picked cotton had better fiber length in Paranapanema-SP and better Micronaire in Araras-SP. Roller gin was better than saw gin for all technological cotton fiber properties and yarn, independent of harvesting processes.

  15. Caracterização postural da jovem praticante de ginástica olímpica Postural characterization of young participants in Olympic gymnastics

    Directory of Open Access Journals (Sweden)

    MMB Guimarães

    2007-06-01

    Full Text Available OBJETIVO: Identificar as alterações posturais em crianças praticantes de ginástica olímpica, com idade entre 8 e 12 anos. MÉTODOS: Foram avaliadas 84 estudantes, 38 praticantes de ginástica olímpica e 46 não praticantes, com idade entre 8 e 12 anos. Foram realizadas fotografias de cada criança nos planos frontal anterior e posterior e plano sagital. As fotos foram analisadas através do software Corel Draw, v. 11.0, utilizando as ferramentas linhas-guia e dimensão (dimensão angular, dimensão vertical e dimensão horizontal, visando determinar os parâmetros das 19 variáveis qualitativas e 5 variáveis quantitativas. RESULTADOS: Ao comparar os sujeitos do grupo teste (GT com os sujeitos do grupo controle (GC, observa-se diferença estatisticamente significante nas variáveis rotação medial de fêmur (GC 56,52%; GT 39,47%, antepulsão de pelve (GC 43,48%; GT 76,32% e rotação de tronco (GC 67,39%; GT 23,68%. Observa-se também diferença significante em relação às medidas de valgo de joelho (GC 4,06 ± 2,32 cm; GT 3,14 ± 1,49cm, desnível de pelve (GC 0,69 ± 0,39 cm; GT 0,53 ± 0,33cm e ângulo tíbio-társico (GC 86,93 ± 2,90 cm; GT 87,11 ± 4,17 cm. CONCLUSÃO: Ao analisar os resultados, nota-se uma tendência ao melhor alinhamento dos membros inferiores no grupo teste quando comparado ao grupo controle. Há um aumento da inclinação pélvica anterior e uma tendência ao aumento da hiperlordose lombar no grupo teste, fatores que podem predispor a praticante de ginástica olímpica a um desalinhamento sobre as estruturas esqueléticas, levando a quadros dolorosos, limitando a sua vida esportiva.OBJECTIVE: To identify the postural alterations among children aged 8 to 12 years who perform Olympic gymnastics. METHOD: Eighty-four students aged 8 to 12 years were assessed: 38 who were participating in Olympic gymnastics and 46 who were not. Each child was photographed in the anterior and posterior frontal planes and in the

  16. Metodologia do ensino de ginástica: novos olhares, novas perspectivas Gym teaching methodology: new approaches, new perspectives Metodología de enseñanza de la gimnasia: Nuevos horizontes, nuevas perspectivas

    Directory of Open Access Journals (Sweden)

    2006-11-01

    Full Text Available Este artigo busca sistematizar uma proposta metodológica para o ensino da Ginástica escolar e comunitária, que vem sendo construída a partir de experiências realizadas junto aos projetos de ensino, pesquisa e extensão vinculados ao Grupo de Estudo, Pesquisa e Experimentação em Ginástica(GEPEGIN, da Faculdade de Educação Física da UFG. A proposta indica alguns princípios, conteúdos e estratégias de ação que possam contribuir para o processo de formação humana crítica, na tentativa de apontar uma nova perspectiva para o ensino, a prática e o conhecimento da Ginástica. PALAVRAS-CHAVE: metodologia da ginástica – formação humana – linguagem corporal. This article aims at systematizing a methodological approach for the teaching of gym in schools and communities, which is being built from the experiences held with teaching, research, and extension (community-bound projects linked to GEPEGIN - the Study, Research, and Gym Experimentation Group, from the Physical Education Department at UFG - the Federal University of Goias. This approach indicates some principles, contents, and strategies for action that might contribute to the process of human education in a critical way, in an attempt to point towards a new perspective for the teaching, the practice, and the knowledge of Gymnastics. KEY-WORDS: gymnastics methodology – human education – body language. Este artículo busca sistematizar una propuesta metodológica para la enseñanza de la Gimnasia escolar y comunitaria, que viene siendo construida a partir de experiencias realizadas junto a los proyectos de enseñanza, pesquisa y extensión vinculados al Grupo de Estudio, Pesquisa y Experimentación en Gimnasia (GEPEGIN, de la Facultad de Educación Física de la UFG. La propuesta indica algunos principios, contenidos y estrategias de acción que puedan contribuir para el proceso de formación humana crítica, intenta señalar una nueva perspectiva para la ense

  17. Regulated eukaryotic DNA replication origin firing with purified proteins.

    Science.gov (United States)

    Yeeles, Joseph T P; Deegan, Tom D; Janska, Agnieszka; Early, Anne; Diffley, John F X

    2015-03-26

    Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated to promote precise genome duplication in every cell cycle. To accomplish this, initiation is partitioned into two temporally discrete steps: a double hexameric minichromosome maintenance (MCM) complex is first loaded at replication origins during G1 phase, and then converted to the active CMG (Cdc45-MCM-GINS) helicase during S phase. Here we describe the reconstitution of budding yeast DNA replication initiation with 16 purified replication factors, made from 42 polypeptides. Origin-dependent initiation recapitulates regulation seen in vivo. Cyclin-dependent kinase (CDK) inhibits MCM loading by phosphorylating the origin recognition complex (ORC) and promotes CMG formation by phosphorylating Sld2 and Sld3. Dbf4-dependent kinase (DDK) promotes replication by phosphorylating MCM, and can act either before or after CDK. These experiments define the minimum complement of proteins, protein kinase substrates and co-factors required for regulated eukaryotic DNA replication. PMID:25739503

  18. Coupling of size-exclusion chromatography to liquid chromatography/mass spectrometry for determination of trace levels of thifensulfuron-methyl and tribenuron-methyl in cottonseed and cotton gin trash.

    Science.gov (United States)

    Stry, J J; Amoo, J S; George, S W; Hamilton-Johnson, T; Stetser, E

    2000-01-01

    Size-exclusion chromatography (SEC) was coupled to reversed-phase liquid chromatography/mass spectrometry for the determination of thifensulfuron-methyl and tribenuron-methyl in cottonseed and cotton gin trash. The limit of quantitation was 20 parts per billion (ppb), and the limit of detection was 6 ppb. The analytes were extracted by homogenization in a buffer solution. The extracts underwent a solvent exchange into methanol and were injected onto an SEC column. As the analytes eluted from the SEC column, the eluate was diverted onto a reversed-phase column for additional separation of the analytes and their detection via mass spectrometry. This method is unique because the samples are not cleaned up before analysis, the analytes are injected in methanol, and the entire analysis is completed in 30 min. Average recoveries and standard deviations for thifensulfuronmethyl and tribenuron-methyl in cotton gin trash were 91 +/- 6% and 88 +/- 5%, respectively. Average recoveries and standard deviations for thifensulfuron-methyl and tribenuron-methyl in cottonseed were 91 +/- 11% and 99 +/- 12%, respectively. This is an effective method for the detection and determination of thifensulfuron-methyl and tribenuron-methyl in cotton. PMID:10868588

  19. Cotton-gin automation by using adaptive fuzzy immune PID controller%基于自适应模糊免疫PID的轧花自动控制系统

    Institute of Scientific and Technical Information of China (English)

    董全成; 冯显英

    2013-01-01

    轧花自动控制是棉花加工过程自动化的重要环节,对棉花加工质量、产量等有着重要影响。以轧花机喂花辊的转速作为控制量,在含潮率、籽棉等级等因素发生变化时,通过调节喂花辊转速,来实时控制喂花量大小,以保持合适的籽棉卷的密度,来达到轧花自动控制的目的。根据轧花工艺特点,用模糊控制、免疫控制与传统PID控制相结合,设计了自适应模糊免疫PID控制系统来控制喂花辊的转速,通过实时修正PID参数来保持控制系统的稳定性。用Matlab对自适应模糊免疫PID、模糊免疫PID和自适应模糊PID 3种控制系统进行了仿真比较分析,同时对基于自适应模糊免疫PID控制的轧花实时运行数据进行了分析,仿真及运行结果表明所设计控制系统在轧花自动控制中是有效和可行的,其在稳定性、鲁棒性上比其他2种智能控制系统性能优越。该控制方法为轧花自动控制提供了一种新的控制策略。%The cotton-gin automation is an important component in the cotton production process and also can affect cotton production quality, output, and profit etc. If keeping a constant seed cotton feed rate, a jam would appeared likely between the rotary knife and ribs of the cotton-gin stand when the moisture regain or seed cotton grade changed. In this paper, the automatic control system was designed that monitored and adjusted the seed cotton feed rate to produce a pre-selected load level on the cotton-gin stand rotary knife while automatically compensating for differences in the seed cotton such as trash and moisture regain content. In this automatic control system, an electrical transducer that is part of the automatic control system measured the input signal from a motor driving the rotary knife, and then proportionately converted and sent this signal as a direct current to the PLC by the AD module. Then the PLC sent the numerical signal to

  20. Avaliação do estado nutricional de atletas de ginástica olímpica do Rio de Janeiro e São Paulo Nutritional status evaluation of olympic gymnastics athletes from Rio de Janeiro and São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Beatriz Gonçalves RIBEIRO

    2002-08-01

    Full Text Available Com o objetivo de avaliar o estado nutricional de atletas de ginástica olímpica, do sexo feminino, foram estudadas 46 ginastas, de 11 a 14 anos, pertencentes a clubes e academias das cidades do Rio de Janeiro e São Paulo. O estudo antropométrico envolveu medidas de massa corporal, estatura, dobras cutâneas e perímetros. Observou-se diferença significativa para as dobras cutâneas tricipital, suprailíaca, da coxa e da panturrilha e para o percentual de gordura (pThe purpose of this study was to evaluate the nutritional status of 46 female olympic gymnastics athletes, aged 11 to 14 years old, attending private gymnastics clubs of the cities of Rio de Janeiro and São Paulo. The anthropometric assessment involved measurements of height, body mass, skinfold thickness and circumferences. Significant difference was observed for the triceps, suprailiac, mid thigh and medial calf skinfolds and for the percentage of body fat (p<0.05. For the assessment of dietary intake a 24-hour recall and a 3-day self-register were used. The energy consumption and the percentage of carbohydrates (50% to 58% in the diet were low and the protein ingestion (1.5 to 1.7 g/kg of body weight was appropriate, according to values proposed for athletes. Vitamin intake was satisfactory, except for folic acid of gymnasts from Rio de Janeiro. From the minerals assessed, the intake of calcium, magnesium, zinc and iron was inadequate. No clinical signs of iron deficiency were observed.

  1. Impacto do beneficiamento sobre o número de neps e quantidade de impurezas da fibra do algodão Ginning impact on the number of neps and amount of cotton fiber contaminants

    Directory of Open Access Journals (Sweden)

    Odilon R. R. F. da Silva

    2010-01-01

    Full Text Available Objetivou-se, neste trabalho, estudar o efeito do beneficiamento sobre o conteúdo de impurezas, pó e o número de neps na pluma, em 12 algodoeiras do Estado de Mato Grosso. O experimento consistiu de uma combinação fatorial de cinco etapas de beneficiamento do algodão em 12 algodoeiras em delineamento inteiramente casualizado com quatro repetições. Amostras de algodão foram coletadas nas seguintes etapas: a no desmanche do fardão; b antes do descaroçamento; c logo após o descaroçamento; d na bica, sem o uso do limpador tipo serrilha; e na bica, após a limpeza da pluma, utilizando-se o limpador tipo serrilha. Em todas as etapas se retiraram quatro amostras padrão de pluma com massa de 350 g a fim de serem analisadas mediante o instrumento AFIS (Advanced Fiber Information System para determinação do conteúdo de impurezas e poeira e o número de neps da fibra. Os resultados indicam que os processos de prelimpeza do algodão em caroço e limpeza da pluma reduzem o conteúdo de impurezas e da poeira do algodão enquanto, juntamente com o descaroçamento, esses processos aumentaram o número de neps da fibra do algodão. A utilização do limpador de pluma tipo serrilha com manta contínua aumenta a eficiência de limpeza da pluma, porém ocasiona incrementos significativos no número de neps.The purpose of this work was to study the ginning effect on the amount of cotton contaminants, dust and neps in cotton fiber, in 12 industries in the Mato Grosso State. The experiment consisted of a factorial combination over five stages of ginning and 12 cotton industries using a randomized design with four replications. Some samples of cotton were collected in the following phases: a in the bale dismantling; b before the ginning; c right after ginning; d into the covered lint slide not using the saw lint cleaner; and e into the covered lint slide, after cleaning process using the saw lint cleaner system. Over all stages four standard samples of

  2. Dictionary of cotton: Picking & ginning

    Science.gov (United States)

    Cotton is an essential commodity for textiles and has long been an important item of trade in the world’s economy. Cotton is currently grown in over 100 countries by an estimated 100 producers. The basic unit of the cotton trade is the cotton bale which consists of approximately 500 pounds of raw c...

  3. Nos rastros da memória de um "Mestre de Ginástica" In the wake of the memory of a "Master of Gymnastics"

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    Janice Zarpellon Mazo

    2010-12-01

    Full Text Available A trajetória de vida de Georg Black, considerado o precursor da Educação Física e dos Esportes no Estado do Rio Grande do Sul, foi marcada por muitas realizações. Por sua relevante contribuição na propagação da ginástica alemã nos clubes esportivos e nas escolas da comunidade alemã de Porto Alegre e São Leopoldo por mais de 30 anos (1905-1937 é apontado por autores (Daudt, 1952; Hofmeister, 1987 como sendo o "Pai da Educação Física no Rio Grande do Sul". Seu nome é lembrado em alguns lugares da cidade de Porto Alegre: Centro Comunitário Georg Black (CEGEB, Grupo de Escoteiros Georg Black e SOGIPA. O objetivo central do presente estudo foi reconstituir a história de vida de Georg Black no cenário da educação física e esportes do Rio Grande do Sul. Para tanto, as fontes consultadas e submetidas à análise documental foram os livros comemorativos da SOGIPA, Atlas do Esporte no Brasil e no Rio Grande do Sul, jornal da Turnerbund, entre outras fontes impressas. A pesquisa revelou que Georg Black, se inseriu de forma ampla no cenário esportivo, especialmente porto-alegrense, contribuindo para a emergência e difusão de práticas corporais e esportivas diversificadas.The trajectory of life of Georg Black, considered the precursor of the Physical Education and the Sports in the state of the Rio Grande Do Sul, was marked by many accomplishments. For its excellent contribution in the propagation of the german gymnastics in the sporting clubs and the schools of the german community of Porto Alegre and São Leopoldo for more than 30 years (1905-1937, some authors (Daudt, 1952; Hofmeister, 1987 it to it mentions as being the "Father of the Physical Education in the Rio Grande Do Sul". Its name is remembered in some places in the city of Porto Alegre: Communitarian center Georg Black (CEGEB, Group of Escoteiros Georg Black and in some sectors of the SOGIPA. In this way, the central objective of the present study was to reconstitute

  4. Detecção e seleção de talentos na ginástica artística feminina: a perspectiva dos técnicos brasileiros

    Directory of Open Access Journals (Sweden)

    Myrian Nunomura

    2014-04-01

    Full Text Available Este artigo tem por objetivo apresentar e discutir a detecção e a seleção de talentos na Ginástica Artística Feminina com enfoque para os aspectos que norteiam as condutas dos técnicos brasileiros. Entrevistamos 34 técnicos de 29 instituições distribuídas no Brasil. Observamos que os meios utilizados para detectar e selecionar os talentos na modalidade privilegiam a idade de ingresso, as características antropométricas e os testes físico-motores. Porém, sabemos que os determinantes do sucesso no esporte são multifatoriais e, por vezes, as avaliações transversais utilizadas não contemplam todos os fatores que contribuem para a obtenção de bons resultados no alto rendimento.

  5. Linguagens do corpo: dimensões expressivas e possibilidades educativas da ginástica e da dança Body languages: expressive dimensions and educational possibilities for gymnastics and dance

    Directory of Open Access Journals (Sweden)

    Lívia Tenorio Brasileiro

    2008-12-01

    Full Text Available O presente texto busca refletir sobre a dança e a ginástica como duas manifestações culturais que, entre outras, constituem o universo das linguagens do corpo, analisando os sentidos e os significados contidos em suas dimensões expressivas e apontando algumas orientações pedagógicas, particularmente para o campo da educação física.The present text is aimed at stimulating a reflection on dance and gymnastics as two different cultural manifestations that, like others, belong to the universe of the body languages. The authors analyze the senses and meanings in the expressive dimension of dance and gymnastics and point to some pedagogical orientations, especially useful to the field of Physical Education.

  6. Effects of a compost made from the solid by-product ("alperujo") of the two-phase centrifugation system for olive oil extraction and cotton gin waste on growth and nutrient content of ryegrass (Lolium perenne L.).

    Science.gov (United States)

    Alburquerque, J A; Gonzálvez, J; García, D; Cegarra, J

    2007-03-01

    A pot experiment was conducted on a low-fertility calcareous soil in order to evaluate the effect on ryegrass growth and nutrient uptake of an organic fertiliser obtained by composting "alperujo" and cotton gin waste. Compost, alone and combined with nitrogen fertilisation, was added to the soil at three rates and three harvests were obtained. The compost application enhanced plant growth in the first and third harvest. However, the additional nitrogen fertilisation clearly improved soil productivity due to the scarce availability of this nutrient in the compost. Also, a general increase in the plant contents of phosphorus and potassium in the first two harvests was recorded, whereas treatments with the maximum compost rate showed the highest plant content of copper in the last two harvests. Decreases in calcium in the last two harvests, in magnesium in all of them and in iron and manganese in the last harvest were also observed. PMID:16762544

  7. Controle postural e uso de informação visual em crianças praticantes e não praticantes de ginástica artística

    OpenAIRE

    André Rocha Viana; José Angelo Barela; Claudia Garcia; Ana Maria Forti Barela

    2011-01-01

    O objetivo deste estudo foi investigar o desempenho e a contribuição da informação visual no controle postural de crianças praticantes e não praticantes de ginástica artística (GA). Quarenta e três meninas, 23 praticantes de GA e 20 não praticantes de GA, idade 5 a 7 e 9 a 11 anos, permaneceram em pé sobre uma plataforma de força em três bases de apoio: bipodal, semi-tandem stance e base reduzida, com olhos abertos e com olhos fechados. A área de deslocamento do centro de pressão e o índice d...

  8. Prática da ginástica laboral por trabalhadores das indústrias do Rio Grande do Sul, Brasil Stretching programs among industrial workers from the state of Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Luana Callegaro Rossato

    2013-03-01

    Full Text Available O presente estudo identificou a prevalência e os fatores associados à prática de ginástica laboral por trabalhadores da indústria no Estado do Rio Grande do Sul, Brasil. Realizou-se análise secundária dos dados de um estudo de delineamento transversal, com amostra probabilística de 2.265 trabalhadores, de ambos os sexos. O instrumento de pesquisa foi o questionário autoadministrado "Estilo de vida e hábitos de lazer dos trabalhadores das indústrias brasileiras", previamente validado. A variável dependente foi a prática de ginástica laboral, definida pela resposta positiva à questão: "Você participa do programa de ginástica na empresa (ginástica laboral?". Foram coletadas informações demográficas, socioeconômicas, comportamentais e relacionadas à saúde, como potenciais fatores associados. Na análise de dados, empregou-se o teste Qui-quadrado de Pearson e o modelo de regressão de Poisson com variância robusta, nas análises bruta e ajustada, respectivamente. A prática de ginástica laboral foi referida por 40,3% dos respondentes (IC95%: 38,2; 42,3, sendo mais prevalente nos trabalhadores do sexo feminino, com maiores níveis de escolaridade, naqueles mais ativos no lazer e que relatavam menores intensidades de esforço no trabalho. Não se observou associação da variável dependente com idade, estado civil, renda familiar bruta, tabagismo, autopercepção de saúde e autopercepção do nível de estresse. Programas de ginástica laboral devem desenvolver estratégias de promoção da saúde que priorizem subgrupos de trabalhadores menos envolvidos, em especial, indivíduos do sexo masculino e com menor escolaridade. Desse modo, será possível reduzir as disparidades, beneficiando trabalhadores, independentemente de suas características sociodemográficas, a melhores condições saúde e bem-estar no ambiente das indústrias.We evaluated the prevalence and factors associated with participation on stretching programs

  9. 棉花不同阶段温度与产量和衣分的相关性研究%Study on Relationships of Yield、Ginning Outturn and Temperature in Different Stages of Cotton

    Institute of Scientific and Technical Information of China (English)

    李春平; 赵萍; 刘忠山; 张大伟; 刘素娟; 徐建辉

    2014-01-01

    The author planted two cotton varieties in the same place from 2006 to 201 2,in order to study on the relationships of monthly mean temperature,the average temperature of ten days,ten days average lowest temperature,the average highest temperature and raw cotton yield,ginned cotton yield and ginning outturn . The results showed that,the raw cotton yield was negative significantly or very significantly correlated with the average highest temperature of mid-June,the average tempera-ture of July,the average temperature of earlier ten days of July and later ten days of July,the average highest temperature of early July and the average highest temperature of mid-July,It was positive significantly or very significantly correlated with the average temperature of late June ,the average lowest temperature of late June,the average highest temperature of late June,the average highest temperature of late July,the average temperature of August,the average lowest temperature of early August,the average temperature of mid-August and the average highest temperature of mid-August . ginned cotton yield was positive significantly or very significantly correlated with the average temperature of late June,the average lowest temperature of late June,the average highest temperature of late June,the average highest temperature of late July,the average highest tem-perature of mid-August and the average highest temperature of mid-August . It was negative significantly or very significantly correlated with the average temperature of early July,the average temperature of late July,the average highest temperature of mid-July . Ginning outturn was positively significantly or very significantly correlated with the average highest temperature of late-July and the average highest temperature of early August . Relationships between temperature and yield,ginning outturn were highly consistent in most of the projects,but the correlations in some projects were significantly different .%2006~2012年

  10. Study on Relationships of Yield、Ginning Outturn and Temperature in Different Stages of Cotton%棉花不同阶段温度与产量和衣分的相关性研究

    Institute of Scientific and Technical Information of China (English)

    李春平; 赵萍; 刘忠山; 张大伟; 刘素娟; 徐建辉

    2014-01-01

    The author planted two cotton varieties in the same place from 2006 to 201 2,in order to study on the relationships of monthly mean temperature,the average temperature of ten days,ten days average lowest temperature,the average highest temperature and raw cotton yield,ginned cotton yield and ginning outturn . The results showed that,the raw cotton yield was negative significantly or very significantly correlated with the average highest temperature of mid-June,the average tempera-ture of July,the average temperature of earlier ten days of July and later ten days of July,the average highest temperature of early July and the average highest temperature of mid-July,It was positive significantly or very significantly correlated with the average temperature of late June ,the average lowest temperature of late June,the average highest temperature of late June,the average highest temperature of late July,the average temperature of August,the average lowest temperature of early August,the average temperature of mid-August and the average highest temperature of mid-August . ginned cotton yield was positive significantly or very significantly correlated with the average temperature of late June,the average lowest temperature of late June,the average highest temperature of late June,the average highest temperature of late July,the average highest tem-perature of mid-August and the average highest temperature of mid-August . It was negative significantly or very significantly correlated with the average temperature of early July,the average temperature of late July,the average highest temperature of mid-July . Ginning outturn was positively significantly or very significantly correlated with the average highest temperature of late-July and the average highest temperature of early August . Relationships between temperature and yield,ginning outturn were highly consistent in most of the projects,but the correlations in some projects were significantly different .%2006~2012年

  11. O livro como ferramenta pedagógica para a inserção da educação física e da ginástica no ensino público primário paranaense (fim do século XIX e início do século XX

    Directory of Open Access Journals (Sweden)

    Diogo Rodrigues Puchta

    2015-09-01

    Full Text Available ResumoNo presente trabalho procuramos analisar as motivações do autor Domingos Nascimento ao se valer da obra de Daniel Schreber Ginástica doméstica, médica e higiênica para escrever sobre a ginástica que deveria ser ensinada e praticada nas escolas paranaenses. Buscamos confrontar a perspectiva dos dois autores com relação à educação física e à ginástica pensadas nos anos iniciais do século XX, bem como contextualizar tais perspectivas com a situação em que se encontrava a instrução pública no Estado do Paraná, de grandes discussões em torno da renovação do ensino, manifestadas no início de um amplo ciclo de reformas escolares, no qual a “novidade” da educação física regular nas escolas é apenas um exemplo.

  12. Lesões desportivas na ginástica artística: estudo a partir de morbidade referida Sports injuries in artistic gymnastics: a study from referred morbidity

    Directory of Open Access Journals (Sweden)

    Rosangela Akemi Hoshi

    2008-10-01

    Full Text Available INTRODUÇÃO: Os níveis de exigência biomecânica devidos ao alto grau de dificuldade na realização de gestos fazem da ginástica artística (GA uma modalidade com elevado risco de lesões. Assim, é necessário que os aspectos a elas relacionados sejam controlados. OBJETIVO: Analisar a ocorrência de lesões na Ginástica Artística, associando-as a fatores de risco específicos da modalidade e do atleta, a partir de inquérito de morbidade referida. MÉTODOS: Foram entrevistados 54 ginastas, recrutados ao acaso, classificados segundo o nível competitivo em duas categorias: regional e nacional. Utilizou-se o inquérito de morbidade referida (IMR com a finalidade de reunir dados sobre a natureza da lesão, região corporal e aparelho ginástico. Os dados foram organizados e apresentados sob a forma de distribuição de freqüências e as variáveis, analisadas segundo nível de associação a partir do teste de Goodman para contrastes entre populações multinomiais, considerando significante o valor P INTRODUCTION: The levels of biomechanical demands due to the high level of difficulty in the gestures accomplishment make Artistic Gymnastics (AG a modality with high risk of injuries. Thus, it is necessary that the aspects concerned with them are controlled. OBJECTIVE: To analyze the occurrence of injuries in artistic gymnastics, associating them with specific risk factors of the modality and of the athlete from reported morbidity survey. METHODS: 54 gymnasts randomly recruited have been interviewed and classified according to their competitive level into two categories: regional and national. The reported morbidity questionnaire (RMQ was used with the purpose to collect data on the injury's nature, body region and gymnastic apparatus. Data were organized and presented under frequency distribution and analyzed variables. The variables were analyzed according to their level of association from the Goodmann's test for contrasts between

  13. Distúrbios de atitudes alimentares e distorção da imagem corporal no contexto competitivo da ginástica rítmica Eating disorders and body image distortion in the rhythmic gymnastics competitive context

    Directory of Open Access Journals (Sweden)

    José Luiz Lopes Vieira

    2009-12-01

    Full Text Available OBJETIVO: Investigar a presença e a relação entre comportamentos sugestivos de transtornos de conduta alimentar e distorção da imagem corporal de atletas de ginástica rítmica participantes do contexto esportivo competitivo. MÉTODOS: Participaram do estudo 48 atletas de ginástica rítmica, das categorias: infantil, juvenil e adulta e 48 estudantes do ensino fundamental e médio (controle. Para a identificação da presença de transtornos de conduta alimentar utilizou-se o Teste de Atitudes Alimentares (EAT-26 e, para determinar a insatisfação com a imagem corporal, o Body Shape Questionnaire (BSQ. Para a análise estatística utilizou-se o teste de Kruskal-Wallis (p OBJECTIVE: The aim of this study was to investigate the presence and relationship between eating behavior disorders and body image distortion in rhythmic gymnastics' athletes who participate in a competitive sports context. METHODS: 48 rhythmic gymnastics athletes from the junior and senior categories and 48 elementary and high school students (control group participated in the study. For the identification of eating behavior disorder the Eating Attitude Test (EAT-26 was used and to determine body image dissatisfaction the Body Shape Questionnaire (BSQ. For statistic analysis the Kruskall-Wallis test (p<0.05 and Spearman Correlation were used. RESULTS: there were not statistical differences between the gymnastics group and the students group for eating behavior disorder; a significant correlation was noticed between eating behavior disorder and body image distortion for the athletes from younger junior (10-12 years old and older junior (12-14 years old sport categories. Descriptive analysis revealed that 27.2% (4 gymnastics/10-12 years old; 20.0% (4 gymnasts/13-14 years old presented suggestive behavior of eating disorder. Two gymnasts aged 10-12 years old (10.0%, five gymnasts aged 13-14 years old (15.0% and five 15 years old gymnasts (100% presented body image distortion

  14. Centro de excelência e ginástica artística feminina: a perspectiva dos técnicos brasileiros Centre of excellence for women artistic gymnastics: the Brazilian coaches' perspective

    Directory of Open Access Journals (Sweden)

    Myrian Nunomura

    2012-06-01

    Full Text Available Durante o período de 2001-2008, a Confederação Brasileira de Ginástica implementou o sistema de seleção permanente concentrada no Centro de Treinamento (CT de Curitiba. Através de um formato semelhante às escolas-internato esportivas da antiga União Soviética, as ginastas passaram a treinar juntas sob o comando de um renomado técnico estrangeiro. Este artigo visa apresentar e discutir o contexto deste CT e o sistema de seleção permanente da ginástica artística feminina (GAF brasileira, a partir da opinião dos técnicos que desenvolvem a modalidade no país. Procedemos a um estudo de campo e entrevistamos 34 treinadores de 29 instituições. Dentre os aspectos positivos da seleção permanente, os sujeitos relacionaram principalmente a infraestrutura disponível às ginastas. Sobre os aspectos negativos, foram citados problemas relacionados ao rigor do treinamento, à polarização e a consequente monopolização das atletas, o que revela que, provavelmente, não houve uma adaptação do modelo soviético às características da GAF desenvolvida no Brasil.During the period of 2001 and 2008, the Brazilian Gymnastics Confederation implemented the gymnasts training boarding center system at the Curitiba Training Center (TC. Using the former Soviet Union model of sports boarding schools, the Brazilian gymnasts started to train together under the supervision of a technical team led by a renowned foreign coach. This article aims to discuss the context of the TC and the system of centralized preparation of the Brazilian women artistic gymnastics (WAG showing the point of view of the coaches. We conducted a field survey and we interviewed 34 coaches of 29 sport institutions. Among the positive aspects, the coaches reported about the better infrastructure available to the gymnasts. The negative aspects refer to the problems regarding rigorous training, the polarization and the consequent monopolization of athletes showing lack of

  15. Desenvolvimento e avaliação de um descaroçador e prensa enfardadeira itinerantes para o beneficiamento do algodão Development of a mobile gin and baler press for cotton

    Directory of Open Access Journals (Sweden)

    Jeane F. Jerônimo

    2013-03-01

    Full Text Available Novas tecnologias vêm sendo incorporadas ao sistema produtivo da cotonicultura brasileira, visando verticalizar a produção por meio do beneficiamento do algodão em caroço possibilitando, aos produtores ou associações, a agregação de valor à sua produção. Objetivou-se então, com o presente trabalho, desenvolver e avaliar um equipamento de beneficiamento composto de descaroçador e prensa enfardadeira itinerantes. O descaroçador se compõe de: limpador, cilindro de serras, cilindro de escovas, condensador e rolo. A prensa enfardadeira é constituída de chapas de prensagem, cilindros hidráulicos, bomba hidráulica, válvula de controle de acionamento, dispositivos de filtragem do óleo hidráulico e manômetro. Esses equipamentos são montados sobre um reboque composto por chassi de cantoneiras em U, quatro rodas de aro 13, pontas de eixo, dois feixes de cinco molas, lastro em chapa de ferro e sistema de frenagem. Na avaliação e validação do conjunto pelos cotonicultores, este conjunto recebeu o conceito "muito bom" quanto ao rendimento do descaroçador, o peso do fardo e em relação à qualidade da fibra obtida. A qualidade da fibra obtida com o conjunto descaroçador e prensa enfardadeira atendeu aos padrões da indústria têxtil.New technologies have been incorporated into the Brazilian cotton production system, in order to verticalize the production through processing, allowing the producers or associations to add value to their production. Thus, the present study aimed to develop and evaluate processing equipment composed of mobile gin and baler press. The ginner consists of cleaner, saw cylinder, cylinder brushes, condenser and coil. The baler press is constituted of pressing sheets, hydraulic cylinders, hydraulic pump, control valve actuation, devices for filtering hydraulic oil and manometer. These devices are mounted on a trailer chassis composed of U-shaped angle iron chassis, four wheels (rim 13, shaft ends, two

  16. As possibilidades da ginástica na sua relação com o lazer e com a sociedade Possibilities of gymnastics in its relation with leisure and society Las posibilidades de la gimnasia en su relación con el ocio y con la sociedad

    Directory of Open Access Journals (Sweden)

    2006-11-01

    Full Text Available Este trabalho apresenta discussão preliminar, tendo como foco as possibilidades da ginástica como vivência no lazer e sua relação com a sociedade e aborda, também, a forma como a ginástica conquistou o espaço das academias, expandindo-se enquanto produto de consumo. O texto aponta a necessidade de se buscar uma excitação agradável na ginástica, necessária para que ela se torne prazerosa, alegre, localizando nela o componente lúdico. Assim, espera-se facilitar a inserção da ginástica no campo do lazer, embora a ludicidade não seja o seu único componente. PALAVRAS-CHAVE: ginástica – lazer – sociedade This research work presents a preliminary discussion focussing on the possibilites of gymnastics as a leisure experience and its relation to society. It also addresses the way through which gymnastics has gained ground in gyms, expanding as a consumption product. This text points to the need of searching for a pleasant excitemente in gymnastics, which is necessary for it to be pleasing and fun, by locating the playful component in it. As such, this text hopes to aid in the implementation of gymnastics in the field of leisure, although playfulness is not its only component. KEYWORDS: gymnastics – leisure – society Este trabajo presenta discusión preliminar, teniendo como foco las posibilidades de la gimnasia como vivencia en el ocio y su relación con la sociedad y aborda, también, la forma como la gimnasia conquistó el espacio de las academias, expandiéndose como producto de consumo. El texto señala la necesidad de buscar una excitación agradable en la gimnasia, necesaria para que ella se torne placentera, alegre, localizando en ella el componente lúdico. Así, se espera facilitar la inserción de la gimnasia en el campo del ocio, aunque la ludicidad no sea su único componente. PALABRAS-CLAVE: gimnasia – ocio – sociedad

  17. Influência do Código de Pontuação no treino da Ginástica Artística Masculina Influence of code of points in men’s gymnastics training

    Directory of Open Access Journals (Sweden)

    Paulo Carrara

    2011-12-01

    Full Text Available O objetivo deste estudo é verificar a influência das novas regras do Código de Pontuação na sistematização do treino da Ginástica Artística Masculina. Para compreender estas alterações, oito treinadores de seleções nacionais, de Portugal e Brasil, foram entrevistados, sobre como essas regras influenciam o treino. Foi utilizada a análise de conteúdo para avaliar os comentários. Os treinadores esclareceram que as novas regras implicaram em mudanças na estrutura das séries e manifestaram novas tendências na sistematização do treino.The aim of this study is to verify the influence of Code of Points new rules on the systematization of Men’s Artistic Gymnastics training. To understand these alterations, eight coaches of Portugal and Brazil national teams were questioned about how these rules affects the training. For the comments, a content analysis was run. The coaches mentioned that the new rules imply changes in routines composition and noticed new tendencies in training systematization.

  18. Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.

    Science.gov (United States)

    Guilliam, Thomas A; Keen, Benjamin A; Brissett, Nigel C; Doherty, Aidan J

    2015-08-18

    Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication. However, recent studies have shown that this perception of the limited activities associated with these diverse enzymes can no longer be justified. Numerous examples can now be cited demonstrating how the term 'DNA primase' only describes a very narrow subset of these nucleotidyltransferases, with the vast majority fulfilling multifunctional roles from DNA replication to damage tolerance and repair. This article focuses on the archaeo-eukaryotic primase (AEP) superfamily, drawing on recently characterized examples from all domains of life to highlight the functionally diverse pathways in which these enzymes are employed. The broad origins, functionalities and enzymatic capabilities of AEPs emphasizes their previous functional misannotation and supports the necessity for a reclassification of these enzymes under a category called primase-polymerases within the wider functional grouping of polymerases. Importantly, the repositioning of AEPs in this way better recognizes their broader roles in DNA metabolism and encourages the discovery of additional functions for these enzymes, aside from those highlighted here.

  19. Consumo de suplementos por alunos de academias de ginástica em São Paulo Supplement consumption among fitness center users in São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Raquel Franzini Pereira

    2003-09-01

    Full Text Available O aumento do número de academias de ginástica na cidade de São Paulo, em conjunto com o aumento da oferta de diferentes suplementos no mercado, despertou interesse para o estudo do consumo de suplementos entre seus alunos. O uso de suplementos pelo público em geral não é bem quantificado e pouca informação sobre este assunto está publicada na literatura. O seu aparecimento no mercado tem sido mais rápido do que a elaboração de regulamentações e a realização de pesquisas científicas que comprovem seus efeitos na saúde dos consumidores e determinem a segurança de seu uso a longo prazo. Em uma amostra de 309 freqüentadores de sete academias de ginástica de São Paulo em 1999, 74 (23,9% consumiam algum tipo de suplemento, dos quais 77,0% eram do sexo masculino e 23,0% do sexo feminino. Os suplementos mais consumidos foram aminoácidos ou outros concentrados protéicos (38,9% e o consumo maior foi o diário (90,3%. A correlação entre gasto com suplemento e renda individual foi de 27,5% (p = 0,0483; n=52, sendo o gasto com suplementos maior entre homens do que entre mulheres; a correlação com renda familiar foi de 36,1% (p = 0,0137; n = 46 e com Índice de Massa Corporal foi de 17,1% (p = 0,1564; n = 70. Conclui-se que o uso de suplementos é significante no grupo analisado, ficando clara a necessidade de novos estudos sobre o consumo desses produtos e seus efeitos, enfocando aspectos de educação nutricional do consumidor de suplementos para aumentar o nível de informação sobre os mesmos e garantir segurança na sua utilização.The increase in the number of fitness centers in the city of São Paulo, together with the increase in the offering of different supplements, has raised interest in the study of the consumption of supplements among fitness center users. The consumption of supplements by the general public has not been well quantified yet, and little information has been published about this subject. The arrival of

  20. Desenvolvimento e validação de um novo sistema de seleção de talentos para a ginástica olímpica feminina: a Bateria PDGO Development and validation of a new system for talent selection in female artistic gymnastics: the PDGO Battery

    OpenAIRE

    Patrícia Arruda de Albuquerque; Paulo de Tarso Veras Farinatti

    2007-01-01

    A ginástica olímpica (GO) envolve atividades motoras que exigem capacidades específicas. Todavia, há carência de testes para detectar o potencial para o desempenho na GO em idades precoces. Este estudo descreve e apresenta evidências da validade de uma bateria de testes para a seleção de talentos para GO feminina. Inicialmente, foram levantadas as atividades que vêm sendo aplicadas por especialistas em GO, na literatura e por consulta através de questionários. A partir das 30 atividades menci...

  1. Caracterização morfológica e funcional das ginastas de artística feminina com idades compreendidas entre os seis e os dez anos de idade Estudo realizado com Ginastas de Alta Competição da Associação de Ginástica do Norte

    OpenAIRE

    Magalhães, Elisabete

    2003-01-01

    Na ginástica, o perfil entendido como o ideal é a forma linear e de baixa estatura. As jovens, prépubertárias ginastas da Europa de Leste, que conseguiram capturar as atenções em 1972 e 1976 nos Jogos Olímpicos, foram cruciais para estabelecer uma nova estandardização no perfil corporal desejado. Esse desejo da figura prepubertária produziu o que alguns observadores chamam de “Nadia’s syndrome”. Este factor foi crucial para introduzir novas pressões nas jovens competidoras, agora com uma form...

  2. Caracterização dos níveis de pressão sonora em academias de ginástica e queixas apresentadas por seus professores Characterization of sound pressure levels in fitness centers and complaints from instructors

    Directory of Open Access Journals (Sweden)

    Adriana B. M. de Lacerda

    2001-09-01

    Full Text Available Objetivo: Este estudo objetiva identificar as queixas auditivas e orgânicas dos professores de ginástica e avaliar os níveis de pressão sonora (NPS produzidos nas aulas de ginástica com música. A medição dos NPS foi realizada seguindo as condições sugeridas pela Associação Brasileira de Normas Técnicas (ABNT, na Norma 10151. Forma de estudo: Prospectivo clínico randomizado. Material e método: 32 professores de ginástica responderam a um questionário com questões referentes às queixas relacionadas aos NPS. Resultados: As queixas mais freqüentes foram: zumbidos (24%, sensação de ouvido tampado (15% e baixa concentração (15%. As modalidades de ginástica nas quais foi realizada a avaliação dos níveis de pressão sonora foram: Step, Body Pump e Aerofitness. Conclusão: Os resultados desta avaliação revelaram intensidades que variaram de 73,9 a 94,2 dBA e sugerem presença de elevados níveis de pressão sonora em 66% das aulas avaliadas.Aim: The present study aims at identifying auditory and health complaints of fitness instructors in fitness centers in Curitiba, and to evaluate the sound pressure levels (SPL produced in fitness classes. SPL measurements were made following ABNT recommendations (Brazilian Technical Norms and Regulations Association - Norm 10151 and revealed levels that ranged from 73.9 to 94.2 dBA. Study design: Prospective clinical randomized. Material and method: 32 fitness instructors answered a questionnaire about their most frequent complaints related to SPL. Results: The most frequent complaints were: tinnitus (24%; ear fullness (15% and poor concentration (15%. The classes in which sound pressure levels were investigated were: Step, Body Pump and Aerofitness. Conclusion: The results of the present research suggested the presence of high sound pressure levels in 66% of the evaluated classes.

  3. Protein Foods

    Science.gov (United States)

    ... Text Size: A A A Listen En Español Protein Foods Foods high in protein such as fish, ... the vegetarian proteins, whether they have carbohydrate. Best Protein Choices The best choices are: Plant-based proteins ...

  4. Uma análise exploratória do processo de orçamento de capital em empresas algodoeiras A qualitative analysis of the capital budgeting process in cotton ginning companies

    Directory of Open Access Journals (Sweden)

    Afonso Carneiro Lima

    2013-06-01

    targeted towards attempting to ensure satisfactory operating results in the short term. Hence, most of the gins investigated seem to be more susceptible to the uncertainties of the environment in which they operate since there is a limited consideration of the possibilities that investments might bring to them, as well as an inherent risk management approach; behavioral aspects such latency were verified in investment decisions performed by the managers interviewed.

  5. Protein-protein interactions

    DEFF Research Database (Denmark)

    Byron, Olwyn; Vestergaard, Bente

    2015-01-01

    . The biophysical and structural investigations of PPIs consequently demand hybrid approaches, implementing orthogonal methods and strategies for global data analysis. Currently, impressive developments in hardware and software within several methodologies define a new era for the biostructural community. Data can......Responsive formation of protein:protein interaction (PPI) upon diverse stimuli is a fundament of cellular function. As a consequence, PPIs are complex, adaptive entities, and exist in structurally heterogeneous interplays defined by the energetic states of the free and complexed protomers...

  6. USING CYCLONES EFFECTIVELY AT COTTON GINS

    Science.gov (United States)

    Cyclones are the most common type of emissions control device used in agricultural processing operations. Cyclones are efficient, reliable, low-cost, and require little maintenance. When used properly, cyclones effectively separate particulate matter from air streams, allowing compliance with state ...

  7. Gin pole in / Jaanika Käst

    Index Scriptorium Estoniae

    Käst, Jaanika

    2008-01-01

    Projektist, mille eesmärgiks sai alaealiste kuritegelikule teele sattumise ennetamine, teadvustades alkoholi, tubakat, narkootikume ja kooli eiramist kui enesehävituslikku riski ning ohtu sattuda kuritegelikule teele

  8. Análise comparativa do equilíbrio unipodal de atletas de ginástica rítmica Comparative analysis of one-foot balance in rhythmic gymnastics athletes

    Directory of Open Access Journals (Sweden)

    Leonardo Shigaki

    2013-04-01

    Full Text Available INTRODUÇÃO: A ginástica rítmica (GR requer alto nível de qualidade física; assim, o bom desempenho é dependente da força e resistência muscular, coordenação motora e equilíbrio postural. OBJETIVO: Desenvolver uma análise comparativa do equilíbrio unipodal de atletas de GR. MÉTODOS: Foram avaliadas dez atletas de GR, do sexo feminino, por meio de uma plataforma de força em apoio unipodal e testes funcionais de equilíbrio (Side Hop Test e Figure of Eight Hop Teste. Para a plataforma, os parâmetros do Centro de Pressão (COP nas direções anteroposterior e mediolateral foram utilizados para análise, enquanto para os testes funcionais, o tempo-segundos de performance. RESULTADOS: Diferença significativa (p = 0,01 foi encontrada entre os membros inferiores no parâmetro de frequência média na direção mediolateral, no qual o membro inferior não dominante apresentou maior estabilidade postural do que o dominante. Para os testes funcionais não houve diferença significativa entre os membros. CONCLUSÃO: A diferença encontrada no controle do equilíbrio na direção mediolateral pode estar relacionada às diferenças nas ações musculares da região do quadril, na qual durante a prática do esporte para estabilização, manutenção do tronco e execução de manobras com os membros inferiores estão presentes. Estes resultados indicam a necessidade de um programa de reequilíbrio e estabilização pélvica para as atletas analisadas no intuito de preservar a simetria muscular dos membros para o bom desempenho esportivo.INTRODUCTION: Rhythmic gymnastics requires a high level of physical quality; therefore, good performance depends on muscular strength and endurance, motor coordination and postural balance. OBJECTIVE: To develop a comparative analysis of postural balance in rhythmic gymnasts. METHODS: 10 female rhythmic gymnasts were evaluated by a force platform on one foot and balance functional tests (Side Hop Test and

  9. Yeast Interacting Proteins Database: YML034W, YDR489W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication mac...it of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and impli

  10. STATISTICAL PROPERTIES OF LONG-RANGE CONTACTS IN GLOBULAR PROTEINS

    Institute of Scientific and Technical Information of China (English)

    Xiang-hong Wang; Jian-hong Ke; Mang-xiao Hu

    2004-01-01

    The analysis of residue-residue contacts in protein structures can shed some light on our understanding of the folding and stability of proteins. In this paper, we study the statistical properties of long-range and short-range residueresidue contacts of 91 globular proteins using CSU software and analyze the importance of long-range contacts in globular protein structure. There are many short-range and long-range contacts in globular proteins, and it is found that the average number of long-range contacts per residue is 5.63 and the percentage of residue-residue contacts which are involved in longrange ones is 59.4%. In more detail, the distribution of long-range contacts in different residue intervals is investigated and it is found that the residues occurring in the interval range of 4-10 residues apart in the sequence contribute more long-range contacts to the stability of globular protein. The number of long-range contacts per residue, which is a measure of ability to form residue-residue contacts, is also calculated for 20 different amino acid residues. It is shown that hydrophobic residues (including Leu, Val, Ile, Met, Phc, Tyr, Cys and Trp) having a large number of long-range contacts easily form long-range contacts, while the hydrophilic amino acids (including Ala, Gly, Thr, His, Glu, Gin, Asp, Asn, Lys, Ser, Arg, and Pro) form long-range contacts with more difficulty. The relationship between the Fauchere-Pliska hydrophobicity scale (FPH) and the number of short-range and long-range contacts per residue for 20 amino acid residues is also studied. An approximately linear relationship between the Fauchere-Pliska hydrophobicity scale (FPH) and the number of long-range contacts per residue CL is found and can be expressed as CiL= a + b x FPHwhere a = 5.04 and b = 1.23. These results can help us to understand the role of residue-residue contacts in globular protein structure.

  11. Total protein

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003483.htm Total protein To use the sharing features on this page, please enable JavaScript. The total protein test measures the total amount of two classes ...

  12. Reproductive biology in monoecious and gynoecious cucumber cultivars as a result of IBA application Biologia reprodutiva de cultivares monóicas e ginóicas de pepino em função do uso de AIB

    Directory of Open Access Journals (Sweden)

    Valdir Diola

    2008-03-01

    concentration.A compreensão da biologia reprodutiva e da regulação da expressão sexual das flores do pepineiro (Cucumis sativus pode facilitar o manejo do cultivo e melhorar a produção e a qualidade dos frutos. O objetivo do presente estudo foi avaliar a resposta fisiológica dos verticilos florais à aplicação de AIB em pepino. O experimento foi conduzido em casa-de-vegetação, em blocos casualizados, com os tratamentos dispostos em fatorial 5 x 2 (AIB nas concentrações de 0, 50, 100, 200 e 500 ìmol, para as cultivares Wisconsin MR28, ginóica, e Caipira, monóica. Foram utilizadas quatro repetições, com quatro plantas por parcela. A cultivar Wisconsin MR28 produziu em média 7.636 grãos de pólen (GP por flor, com resposta linear positiva ao aumento nas concentrações de AIB. Já na cultivar Caipira (5.160 GP, todas as concentrações de AIB interferiram negativamente na produção de GP. As cultivares não diferiram entre si e em ambas houve efeito linear positivo da concentração de AIB sobre a viabilidade dos GP (germinação média in vitro de 75,4 e 79,9% para as cultivares Wisconsin MR28 e Caipira, respectivamente. As cultivares Wisconsin MR28 e Caipira apresentaram em média 71,6 e 70,6 sementes por fruto respectivamente, respondendo com aumento no número de sementes e melhoria do aspecto do fruto ao incremento da concentração de AIB. A relação de flores masculinas:femininas diferiu significativamente entre cultivares (4,2:1 e 3,5:1 respectivamente para as cultivares Wisconsin MR28 e Caipira e foi afetada de forma distinta nas duas cultivares pelas concentrações de AIB: concentrações próximas de 100 µmol aumentaram o número de flores masculinas na cultivar Caipira e reduziram na cultivar Wisconsin MR28. A quantidade de flores femininas foi semelhante entre as cultivares e relativamente constante na cultivar Caipira nas diferentes concentrações de AIB. Já na cultivar Wisconsin MR 28, houve aumento do número de flores femininas em fun

  13. Protein Structure

    Science.gov (United States)

    Asmus, Elaine Garbarino

    2007-01-01

    Individual students model specific amino acids and then, through dehydration synthesis, a class of students models a protein. The students clearly learn amino acid structure, primary, secondary, tertiary, and quaternary structure in proteins and the nature of the bonds maintaining a protein's shape. This activity is fun, concrete, inexpensive and…

  14. Ginástica rítmica – análise dos fatores competitivos motivadores e estressantes da Seleção Brasileira Juvenil =Rhythmical gymnastics – analysis of competitive, motivating and stress factors of the teenager Brazilian National Football Team

    Directory of Open Access Journals (Sweden)

    Lenamar Fiorese Vieira

    2005-07-01

    Full Text Available Este estudo objetivou investigar a trajetória de desenvolvimento das atletas de ginástica rítmica da Seleção Brasileira Juvenil, com foco na identificação dos fatores competitivos (motivadores e estressantes que ocorrem em diferentes fases da carreira esportiva. A população alvo foi de dez atletas. Como instrumentos de medida foramutilizados o Teste de Carga Psíquica de Frester e entrevista semi-estruturada. Para análise utilizou-se estatística descritiva e a análise de conteúdo do tipo categorial. Os resultados demonstraram que na ginástica rítmica o processo de especialização motora das atletas inicia aos 12 anos; os fatores motivadores evidenciados foram o gosto pelo esporte e ser o favorito; os fatores estressantes foram ser prejudicado pelos juizes e falta de tempo para divertimento.Concluindo em uma análise geral, as atletas perceberam o contexto esportivo competitivo como mais estressante do que motivador; esta afirmação acentuou-se a partir da fase de prática motora em que o estresse competitivo para o rendimento se intensificou.This study aimed to investigate the developmental trajectory of the Brazilian Rhythmic Gymnastics Youth Team, focusing on the identification of competitive factors (motivator and stressful thatoccurs in different sport’s career phases. The sample was composed ten athletes. As measure instruments were used the Frester’s Test Psychic Load and a semi-structured interview. The data were analyzed through the descriptive statistics and categorical type of content’s analysis. The results demonstrated that in the rhythmic gymnastics the process of the athletes’ motor specialization begins at 12 years; the motivator factors evidenced were fun for sport and being the favorite; the stressful factors were being harmed by the judges and not time for fun. Concluding in a general analysis the athletes perceived the competitive sporting context as more stressful than motivator; this affirmation

  15. Principles of protein-protein interactions.

    OpenAIRE

    Jones, S; Thornton, J. M.

    1996-01-01

    This review examines protein complexes in the Brookhaven Protein Databank to gain a better understanding of the principles governing the interactions involved in protein-protein recognition. The factors that influence the formation of protein-protein complexes are explored in four different types of protein-protein complexes--homodimeric proteins, heterodimeric proteins, enzyme-inhibitor complexes, and antibody-protein complexes. The comparison between the complexes highlights differences tha...

  16. Tau protein

    DEFF Research Database (Denmark)

    Frederiksen, Jette Lautrup Battistini; Kristensen, Kim; Bahl, Jmc;

    2011-01-01

    Background: Tau protein has been proposed as biomarker of axonal damage leading to irreversible neurological impairment in MS. CSF concentrations may be useful when determining risk of progression from ON to MS. Objective: To investigate the association between tau protein concentration and 14......-3-3 protein in the cerebrospinal fluid (CSF) of patients with monosymptomatic optic neuritis (ON) versus patients with monosymptomatic onset who progressed to multiple sclerosis (MS). To evaluate results against data found in a complete literature review. Methods: A total of 66 patients with MS and/or ON from...... the Department of Neurology of Glostrup Hospital, University of Copenhagen, Denmark, were included. CSF samples were analysed for tau protein and 14-3-3 protein, and clinical and paraclinical information was obtained from medical records. Results: The study shows a significantly increased concentration of tau...

  17. Protein-Protein Interaction Databases

    DEFF Research Database (Denmark)

    Szklarczyk, Damian; Jensen, Lars Juhl

    2015-01-01

    of research are explored. Here we present an overview of the most widely used protein-protein interaction databases and the methods they employ to gather, combine, and predict interactions. We also point out the trade-off between comprehensiveness and accuracy and the main pitfall scientists have to be aware...

  18. Yeast Interacting Proteins Database: YJL072C, YDR489W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available alized to DNA replication origins and implicated in assembly of the DNA replication machinery Rows with this...f2p, Psf3p), which is localized to DNA replication origins and implicated in asse...the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins...sf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machi

  19. Yeast Interacting Proteins Database: YDR013W, YDR489W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available alized to DNA replication origins and implicated in assembly of the DNA replication machinery Rows with this...f2p, Psf3p), which is localized to DNA replication origins and implicated in asse... the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins...Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication mach

  20. Protein Crystallization

    Science.gov (United States)

    Chernov, Alexander A.

    2005-01-01

    Nucleation, growth and perfection of protein crystals will be overviewed along with crystal mechanical properties. The knowledge is based on experiments using optical and force crystals behave similar to inorganic crystals, though with a difference in orders of magnitude in growing parameters. For example, the low incorporation rate of large biomolecules requires up to 100 times larger supersaturation to grow protein, rather than inorganic crystals. Nucleation is often poorly reproducible, partly because of turbulence accompanying the mixing of precipitant with protein solution. Light scattering reveals fluctuations of molecular cluster size, its growth, surface energies and increased clustering as protein ages. Growth most often occurs layer-by-layer resulting in faceted crystals. New molecular layer on crystal face is terminated by a step where molecular incorporation occurs. Quantitative data on the incorporation rate will be discussed. Rounded crystals with molecularly disordered interfaces will be explained. Defects in crystals compromise the x-ray diffraction resolution crucially needed to find the 3D atomic structure of biomolecules. The defects are immobile so that birth defects stay forever. All lattice defects known for inorganics are revealed in protein crystals. Contribution of molecular conformations to lattice disorder is important, but not studied. This contribution may be enhanced by stress field from other defects. Homologous impurities (e.g., dimers, acetylated molecules) are trapped more willingly by a growing crystal than foreign protein impurities. The trapped impurities induce internal stress eliminated in crystals exceeding a critical size (part of mni for ferritin, lysozyme). Lesser impurities are trapped from stagnant, as compared to the flowing, solution. Freezing may induce much more defects unless quickly amorphysizing intracrystalline water.

  1. Prediction of Protein-Protein Interactions Related to Protein Complexes Based on Protein Interaction Networks

    OpenAIRE

    Peng Liu; Lei Yang; Daming Shi; Xianglong Tang

    2015-01-01

    A method for predicting protein-protein interactions based on detected protein complexes is proposed to repair deficient interactions derived from high-throughput biological experiments. Protein complexes are pruned and decomposed into small parts based on the adaptive k-cores method to predict protein-protein interactions associated with the complexes. The proposed method is adaptive to protein complexes with different structure, number, and size of nodes in a protein-protein interaction net...

  2. Protein electrophoresis - serum

    Science.gov (United States)

    ... of protein and fat, called lipoproteins (such as LDL cholesterol). ... globulin proteins may indicate: Abnormally low level of LDL cholesterol Malnutrition Increased gamma globulin proteins may indicate: Bone ...

  3. As práticas escolares e a formação cívico-patriótica no ginásio mineiro de Uberlândia, em Minas Gerais, Brasil (1920-1970 - School practices and civic and patriotic education in lower secondary school in Uberlândia, Minas Gerais, Brazil (1920/1970

    Directory of Open Access Journals (Sweden)

    Giseli Cristina do Vale Gatti, Geraldo Inácio Filho

    2011-03-01

    Full Text Available Resumo Trata-se do exame  das  práticas  escolares efetivadas no Ginásio Mineiro de  Uberlândia,  em  Minas Gerais, Brasil, no período compreendido entre as décadas de 1920  e de 1970.  Depreende-se do cotejamento das evidências (manuscritas, impressas, orais e iconográficas com  o  contexto  sócio-histórico  da   época   que:  a metodologia de ensino no período era tradicional; a consolidação de uma representação social da  qualidade do ensino do antigo Ginásio (com  caráter   humanístico  e  com  preocupação  com  a  moral,  a disciplina e  os  bons  costumes; o  entendimento  da  escola como centro social, esportivo e cultural da  cidade,  sendo as datas cívicas muito comemoradas. Desse modo, ao conteúdo humanístico, ligado à disseminação de princípios morais aceitos socialmente, somava-se a ênfase no civismo e no patriotismo. Palavras-chave: escola; civismo; patriotismo; currículo; práticas.   SCHOOL PRACTICES AND CIVIC AND PATRIOTIC EDUCATION IN LOWER SECONDARY SCHOOL  IN UBERLANDIA, MINAS GERAIS, BRAZIL (1920/1970 Abstract This  study  is  an  examination  of  school  practices in  the  Lower Secondary  School  in  Uberlandia,  Minas  Gerais,  Brazil,  in  the decades from the 1920s  to 1970s. Conclusions are drawn from the collating of  evidence (manuscript,  printed,  oral and  iconographic with the social historical context of the time in which: the teaching methodology was traditional, consolidation of a social representation of  the  quality  of  teaching  of  the   ancient  Gymnasium  (with  a humanistic  character  and  concern  with morals,  discipline e  good habits; the  school as the social, sporting and cultural center of the city with the civic dates being highly commemorated. In this way, the emphasis  on   civic  education  and   patriotism   was  added  to   a humanistic  content  connected  with  the

  4. Grafting of protein-protein binding sites

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A strategy for grafting protein-protein binding sites is described. Firstly, key interaction residues at the interface of ligand protein to be grafted are identified and suitable positions in scaffold protein for grafting these key residues are sought. Secondly, the scaffold proteins are superposed onto the ligand protein based on the corresponding Ca and Cb atoms. The complementarity between the scaffold protein and the receptor protein is evaluated and only matches with high score are accepted. The relative position between scaffold and receptor proteins is adjusted so that the interface has a reasonable packing density. Then the scaffold protein is mutated to corresponding residues in ligand protein at each candidate position. And the residues having bad steric contacts with the receptor proteins, or buried charged residues not involved in the formation of any salt bridge are mutated. Finally, the mutated scaffold protein in complex with receptor protein is co-minimized by Charmm. In addition, we deduce a scoring function to evaluate the affinity between mutated scaffold protein and receptor protein by statistical analysis of rigid binding data sets.

  5. Estado emocional de alunos da primeira série de um Grupo Escolar - Ginásio da cidade de São Paulo, Brasil: problemática de saúde pública? Emotional disturbances of 1st grade school children: a Public Health Problem?

    Directory of Open Access Journals (Sweden)

    Helena Savastano

    1977-12-01

    Full Text Available Estudou-se uma amostra representativa de alunos da 1.ª série de um Grupo Escolar-Ginásio da rede estadual da cidade de São Paulo, com o objetivo de compreender a dinâmica emocional dessas crianças. Os alunos foram estratificados em fracos e não fracos quanto à aprendizagem e disciplinamento. Utilizou-se o teste de apercepção temática de Bellak e Bellak e os resultados demonstraram, que 43% das crianças não estão emocionalmente ajustadas para enfrentar o ambiente escolar, onde a figura do adulto (professor é visualizada como agressiva e os castigos são vistos, pelas crianças, como ameaçadores; os alunos fracos revelaram maior atraso no desenvolvimento psicossocial em relação aos não fracos. Estes dados, possivelmente, esclarecem parte das dificuldades de adaptação dessas crianças �� situação escolar e sugerem: 1º maior atenção dos técnicos de educação, de saúde e áreas correlatas, para esses problemas; 2º maiores conhecimentos pelos educadores, dos aspectos do desenvolvimento psicossocial da criança.Psychological and epidemiological aspects of emotional dynamics of children enroled in the first grade of a state school of the city of São Paulo, Brazil, were studied based on results of Children's Tematic Test (CAT, of Bellak and Bellak. The mentioned test showed that the group is made up of emotionally immature children, lacking affection, especially those considered of low learning capacity. The figure of the adult, as seen by the students, is inadequate, punitive, indiferent and agressive. The results suggest that teachers should have a greater understanding reference to such problems and should give attention to these students, thus leading to the conclusion that first grade classes should not have so many students. The results also suggest that parents should have more knowledge and understanding as regards child development from conception, as well as better family struture as to more adequate

  6. Ginástica rítmica: análise dos fatores competitivos motivadores e estressantes da Seleção Brasileira Juvenil - DOI: 10.4025/actascihealthsci.v27i2.1419 Rhythmical gymnastics: analysis of competitive, motivating and stress factors of the teenager Brazilian National Football Team - DOI: 10.4025/actascihealthsci.v27i2.1419

    Directory of Open Access Journals (Sweden)

    Marise Botti

    2005-03-01

    Full Text Available Este estudo objetivou investigar a trajetória de desenvolvimento das atletas de ginástica rítmica da Seleção Brasileira Juvenil, com foco na identificação dos fatores competitivos (motivadores e estressantes que ocorrem em diferentes fases da carreira esportiva. A população alvo foi de dez atletas. Como instrumentos de medida foram utilizados o Teste de Carga Psíquica de Frester e entrevista semi-estruturada. Para análise utilizou-se estatística descritiva e a análise de conteúdo do tipo categorial. Os resultados demonstraram que na ginástica rítmica o processo de especialização motora das atletas inicia aos 12 anos; os fatores motivadores evidenciados foram o gosto pelo esporte e ser o favorito; os fatores estressantes foram ser prejudicado pelos juizes e falta de tempo para divertimento. Concluindo em uma análise geral, as atletas perceberam o contexto esportivo competitivo como mais estressante do que motivador; esta afirmação acentuou-se a partir da fase de prática motora em que o estresse competitivo para o rendimento se intensificouThis study aimed to investigate the developmental trajectory of the Brazilian Rhythmic Gymnastics Youth Team, focusing on the identification of competitive factors (motivator and stressful that occurs in different sport’s career phases. The sample was composed ten athletes. As measure instruments were used the Frester’s Test Psychic Load and a semi-structured interview. The data were analyzed through the descriptive statistics and categorical type of content’s analysis. The results demonstrated that in the rhythmic gymnastics the process of the athletes’ motor specialization begins at 12 years; the motivator factors evidenced were fun for sport and being the favorite; the stressful factors were being harmed by the judges and not time for fun. Concluding in a general analysis the athletes perceived the competitive sporting context as more stressful than motivator; this affirmation

  7. Detecting overlapping protein complexes in protein-protein interaction networks

    OpenAIRE

    Nepusz, Tamás; Yu, Haiyuan; Paccanaro, Alberto

    2012-01-01

    We introduce clustering with overlapping neighborhood expansion (ClusterONE), a method for detecting potentially overlapping protein complexes from protein-protein interaction data. ClusterONE-derived complexes for several yeast data sets showed better correspondence with reference complexes in the Munich Information Center for Protein Sequence (MIPS) catalog and complexes derived from the Saccharomyces Genome Database (SGD) than the results of seven popular methods. The results also showed a...

  8. Fusion-protein-assisted protein crystallization.

    Science.gov (United States)

    Kobe, Bostjan; Ve, Thomas; Williams, Simon J

    2015-07-01

    Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the `heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation. In another approach, the `fusion of interacting proteins approach', protein assemblies can be stabilized by covalently linking the interacting partners. The linker connecting the proteins plays different roles in the two applications: in the first approach a rigid linker is required to reduce conformational heterogeneity; in the second, conversely, a flexible linker is required that allows the native interaction between the fused proteins. The two approaches can also be combined. The recent applications of fusion-protein technology in protein crystallization from the work of our own and other laboratories are briefly reviewed.

  9. EDITORIAL: Precision proteins Precision proteins

    Science.gov (United States)

    Demming, Anna

    2010-06-01

    Since the birth of modern day medicine, during the times of Hippocrates in ancient Greece, the profession has developed from the rudimentary classification of disease into a rigorous science with an inspiring capability to treat and cure. Scientific methodology has distilled clinical diagnostic tools from the early arts of prognosis, which used to rely as much on revelation and prophecy, as intuition and judgement [1]. Over the past decade, research into the interactions between proteins and nanosystems has provided some ingenious and apt techniques for delving into the intricacies of anatomical systems. In vivo biosensing has emerged as a vibrant field of research, as much of medical diagnosis relies on the detection of substances or an imbalance in the chemicals in the body. The inherent properties of nanoscale structures, such as cantilevers, make them well suited to biosensing applications that demand the detection of molecules at very low concentrations. Measurable deflections in cantilevers functionalised with antibodies provide quantitative indicators of the presence of specific antigens when the two react. Such developments have roused mounting interest in the interactions of proteins with nanostructures, such as carbon nanotubes [3], which have demonstrated great potential as generic biomarkers. Plasmonic properties are also being exploited in sensing applications, such as the molecular sentinel recently devised by researchers in the US. The device uses the plasmonic properties of a silver nanoparticle linked to a Raman labelled hairpin DNA probe to signal changes in the probe geometry resulting from interactions with substances in the environment. Success stories so far include the detection of two specific genes associated with breast cancer [4]. A greater understanding of how RNA interference regulates gene expression has highlighted the potential of using this natural process as another agent for combating disease in personalized medicine. However, the

  10. Protein Crystal Based Nanomaterials

    Science.gov (United States)

    Bell, Jeffrey A.; VanRoey, Patrick

    2001-01-01

    This is the final report on a NASA Grant. It concerns a description of work done, which includes: (1) Protein crystals cross-linked to form fibers; (2) Engineering of protein to favor crystallization; (3) Better knowledge-based potentials for protein-protein contacts; (4) Simulation of protein crystallization.

  11. Shotgun protein sequencing.

    Energy Technology Data Exchange (ETDEWEB)

    Faulon, Jean-Loup Michel; Heffelfinger, Grant S.

    2009-06-01

    A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.

  12. Protein-Protein Interaction Analysis by Docking

    OpenAIRE

    Stephan Ederer; Florian Fink; Wolfram Gronwald

    2009-01-01

    Based on a protein-protein docking approach we have developed a procedure to verify or falsify protein-protein interactions that were proposed by other methods such as yeast-2-hybrid assays. Our method currently utilizes intermolecular energies but can be expanded to incorporate additional terms such as amino acid based pair-potentials. We show some early results that demonstrate the general applicability of our approach.

  13. Protein folding, protein homeostasis, and cancer

    Institute of Scientific and Technical Information of China (English)

    John H. Van Drie

    2011-01-01

    Proteins fold into their functional 3-dimensional structures from a linear amino acid sequence. In vitro this process is spontaneous; while in vivo it is orchestrated by a specialized set of proteins, called chaperones. Protein folding is an ongoing cellular process, as cellular proteins constantly undergo synthesis and degradation. Here emerging links between this process and cancer are reviewed. This perspective both yields insights into the current struggle to develop novel cancer chemotherapeutics and has implications for future chemotherapy discovery.

  14. Protein-protein complexation in bioluminescence

    OpenAIRE

    Titushin, Maxim S.; Feng, Yingang; Lee, John; Vysotski, Eugene S.; Liu, Zhi-jie

    2011-01-01

    In this review we summarize the progress made towards understanding the role of protein-protein interactions in the function of various bioluminescence systems of marine organisms, including bacteria, jellyfish and soft corals, with particular focus on methodology used to detect and characterize these interactions. In some bioluminescence systems, protein-protein interactions involve an “accessory protein” whereby a stored substrate is efficiently delivered to the bioluminescent enzyme lucife...

  15. Protein-losing enteropathy

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/007338.htm Protein-losing enteropathy To use the sharing features on this page, please enable JavaScript. Protein-losing enteropathy is an abnormal loss of protein ...

  16. Protein and Heart Health

    Science.gov (United States)

    ... Recognition & Awards Healthy Workplace Food and Beverage Toolkit Protein and Heart Health Updated:May 5,2015 Protein ... said. What’s the harm in getting too much protein? The main problem is that often the extra ...

  17. Oligomeric protein structure networks: insights into protein-protein interactions

    Directory of Open Access Journals (Sweden)

    Brinda KV

    2005-12-01

    Full Text Available Abstract Background Protein-protein association is essential for a variety of cellular processes and hence a large number of investigations are being carried out to understand the principles of protein-protein interactions. In this study, oligomeric protein structures are viewed from a network perspective to obtain new insights into protein association. Structure graphs of proteins have been constructed from a non-redundant set of protein oligomer crystal structures by considering amino acid residues as nodes and the edges are based on the strength of the non-covalent interactions between the residues. The analysis of such networks has been carried out in terms of amino acid clusters and hubs (highly connected residues with special emphasis to protein interfaces. Results A variety of interactions such as hydrogen bond, salt bridges, aromatic and hydrophobic interactions, which occur at the interfaces are identified in a consolidated manner as amino acid clusters at the interface, from this study. Moreover, the characterization of the highly connected hub-forming residues at the interfaces and their comparison with the hubs from the non-interface regions and the non-hubs in the interface regions show that there is a predominance of charged interactions at the interfaces. Further, strong and weak interfaces are identified on the basis of the interaction strength between amino acid residues and the sizes of the interface clusters, which also show that many protein interfaces are stronger than their monomeric protein cores. The interface strengths evaluated based on the interface clusters and hubs also correlate well with experimentally determined dissociation constants for known complexes. Finally, the interface hubs identified using the present method correlate very well with experimentally determined hotspots in the interfaces of protein complexes obtained from the Alanine Scanning Energetics database (ASEdb. A few predictions of interface hot

  18. Drugging Membrane Protein Interactions.

    Science.gov (United States)

    Yin, Hang; Flynn, Aaron D

    2016-07-11

    The majority of therapeutics target membrane proteins, accessible on the surface of cells, to alter cellular signaling. Cells use membrane proteins to transduce signals into cells, transport ions and molecules, bind cells to a surface or substrate, and catalyze reactions. Newly devised technologies allow us to drug conventionally "undruggable" regions of membrane proteins, enabling modulation of protein-protein, protein-lipid, and protein-nucleic acid interactions. In this review, we survey the state of the art of high-throughput screening and rational design in drug discovery, and we evaluate the advances in biological understanding and technological capacity that will drive pharmacotherapy forward against unorthodox membrane protein targets. PMID:26863923

  19. Field Effect Transistor /FET/ circuit for variable gin amplifiers

    Science.gov (United States)

    Spaid, G. H.

    1969-01-01

    Amplifier circuit using two FETs combines improved input and output impedances with relatively large signal handling capability and an immunity from adverse effects of automatic gain control. Circuit has sources and drains in parallel plus a resistive divider for signal and bias to either of the gate terminals.

  20. Uncertainty analys for cotton gin PM10 emissions

    Science.gov (United States)

    Due to EPA’s implementation of more stringent standards for particulate matter (PM) with an effective diameter less than 2.5 microns (PM2.5) and the limited availability of PM2.5 emissions data, the cotton ginners’ associations across the cotton belt agreed that there is an urgent need to collect gi...

  1. 'But Yet and Still the Cotton Gin Kept on Working...'

    Science.gov (United States)

    Walker, Alice

    1983-01-01

    Describes the work of Friends of the Children of Mississippi, a Head Start program which began in the late 1960s and for which the author was hired to develop Black history materials for teacher training. Presents excerpts from the autobiographies of several Black women trainees in the program. (GC)

  2. Ma räägin sulle loo / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2011-01-01

    Urmas Vadi (autor ja lavastaja) lavastusest "Minu isa 20 aastat hiljem" Ugala teatri kohvikus ja Norra näitekirjaniku Jon Fosse näidendi „Üks suvepäev” lavastusest Theatrumis, lavastaja Lembit Peterson

  3. Novas Tecnologias e Novas Formas de Organização do Trabalho do Professor nas Academias de Ginástica New Technologies and New Ways of Organizing the Work of the Teacher at Sports Centers Nuevas Tecnologías y Nuevas Formas en Organizacion de Trabajo de Profesor en Gimnasios de Gimnasia

    Directory of Open Access Journals (Sweden)

    2007-09-01

    Full Text Available A partir de um estudo de caso realizado em uma grande academia de Goiânia, trago este recorte com o objetivo de compreender como as transformações tecnológicas nas academias de ginástica têm influenciado a organização do trabalho neste espaço. A pesquisa foi desenvolvida no primeiro semestre de 2004. Para coleta de dados foram realizadas entrevistas semi-estruturadas com professores da academia pesquisada. A academia escolhida, cujo nome fictício será Academia X, foi a que representa o que há e mais avançado nesse ramo de atividade em Goiânia. Assim, poderão ser apontadas algumas tendências para as demais academias de acordo com os resultados obtidos. Isso só é possível porque a tendência das academias menos desenvolvidas é acompanhar as mais desenvolvidas. PALAVRAS-CHAVE: academia – tecnologia – trabalho. Based on a case study undertaken in a large gym in the city of Goiânia, Brazil, I present this paper that aims at understanding how technological changes in gyms have infl uenced the organization of work in such spaces. This research was developed in the first quarter of 2004. Semi-structured interviews with teachers from the investigated gym were done for data collection. The chosen gym, herein renamed as Academia X, was the one that had the most advanced technology available for this business sector in the city of Goiânia. The achieved results may point to future trends for the other gyms. This is only possible because lesser developed gyms tend to match their more developed counterparts. KEYWORDS: gym – technology – work. A partir de un estudio de caso realizado en una gran academia de la ciudad de Goiânia, traigo este resumen con el objetivo de compreender cómo las transformaciones tecnológicas en las academias de gimnasia han infl uenciado la organización del trabajo en este espacio. La investigación fue desarrollada en el primer semestre de 2004. Para adquirir datos fueron realizadas entrevistas semi

  4. Prevalência do uso e conhecimento de esteroides anabolizantes androgênicos por estudantes e professores de educação física que atuam em academias de ginástica Prevalence of the use of anabolic androgenic steroids by physical education students and teachers who work in health clubs

    Directory of Open Access Journals (Sweden)

    Odilon Salim Costa Abrahin

    2013-02-01

    Full Text Available INTRODUÇÃO: Os esteroides anabólicos androgênicos (EAA são substâncias, quimicamente semelhantes à testosterona, utilizadas para o tratamento/controle de diversas doenças. Contudo, tais substâncias estão sendo empregadas de forma não terapêutica e indiscriminada com finalidades de melhora da performance esportiva e principalmente estética. OBJETIVO: analisar a prevalência do uso e o conhecimento de EAA por estudantes e professores de educação física que atuam em academias de ginástica de Belém, PA. Utilizou-se para a coleta de dados um questionário fechado e anônimo, aplicado a 117 pesquisados. A comparação da prevalência do uso e o grau de conhecimento dos pesquisados sobre EAA foi realizada através de estatística não paramétrica, prova de X² (Qui-quadrado, considerando o intervalo de confiança de 95% e p Anabolic androgenic steroids (AAS are chemically similar to testosterone, used for the treatment/control of various diseases. However, these substances are being used in non-therapeutic and indiscriminate purposes to improve sports performance and mainly esthetics. This study aimed to analyze the prevalence of AAS use and information of undergraduates and physical education teachers working in fitness centers in Belém-PA. A closed anonymous questionnaire was applied to 117 volunteers as an instrument. Comparison of the prevalence of use and degree of information of the respondents about AAS was performed using statistical non-parametric test X² (chi-square, considering the range of 95%, significant when p<0.05. The average age of the participants was 28.0 ± 6.3 years and the prevalence of AAS use was of 31.6%. The highest prevalence found was among specialist professionals (39.3%, the main motivation for the use of AAS was 75.6% to esthetics. Regarding the information, it was found that the drugs were classified as AAS: Durateston, Deca-Durabolin, Oxandrolona/Winstrol. However, these professionals took other

  5. Confiabilidade intra e interexaminadores da fotogrametria na classificação do grau de lipodistrofia ginóide em mulheres assintomáticas Intra and inter-examiner reliability of photogrammetry in the classification of the degree of gynoid lypodystrophy in asymptomatic women

    Directory of Open Access Journals (Sweden)

    Ana Maria da Silva Mendonça

    2009-06-01

    Full Text Available A lipodistrofia ginóide (celulite é uma afecção dermatológica comum entre as mulheres. Uma de suas formas de avaliação é a inspeção visual por meio da fotogrametria. Entretanto, não foram encontrados estudos que verifiquem a repetibilidade e reprodutibilidade dessa avaliação. O objetivo deste estudo foi verificar a confiabilidade intra e interexaminadores da avaliação da celulite por meio da fotogrametria. Foram fotografadas e avaliadas as regiões glúteas de 50 mulheres (26,14±4,45 anos. A reprodutibilidade foi testada pela avaliação da mesma fotografia por dois examinadores em duas ocasiões diferentes, com intervalo de um ano; a repetibilidade por um mesmo examinador, com intervalo de uma semana. Os dados foram analisados estatisticamente e utilizado o índice Kappa ponderado. Os resultados indicaram substancial correlação (κ=0,70 entre os avaliadores para o grau de celulite no glúteo superior e moderada correlação (κ=0,50 para o grau de celulite no glúteo inferior. Nas análises de repetibilidade, os dados demonstraram excelente correlação (κ=0,81 para o grau de celulite no glúteo superior e substancial correlação (κ=0,75 para os graus no glúteo inferior. O método proposto para qualificação dos diferentes graus de celulite pela fotogrametria apresentou confiabilidade aceitável intra e interexaminadores para a maioria das regiões avaliadas, com exceção da região do glúteo inferior.Gynoid lypodystrophy (cellulitis is a common condition among women. One of its forms of evaluation is the visual inspection by photogrammetry. However, no studies could be found in literature on the repeatability and reproducibility of such evaluation. The purpose of this study was to assess reliability intra and inter-examiner of cellulitis evaluation by photogrammetry. The gluteal regions of 50 women (mean age 26.14±4.45 years were photographed and evaluated. Reproducibility was tested by evaluation of the same

  6. A influência do ciclo menstrual na flexibilidade em praticantes de ginástica de academia La influencia del ciclo menstrual sobre la flexibilidad en practicantes de gimnasia de academia The influence of the menstrual cycle on the flexibility in practitioners of gymnastics at fitness centers

    Directory of Open Access Journals (Sweden)

    Solange Mattos Melegario

    2006-06-01

    Full Text Available Este estudo teve por objetivo investigar se existem diferenças no grau de flexibilidade nas fases do ciclo menstrual de mulheres adultas jovens praticantes de ginástica de academia. A amostra intencional foi composta de 20 mulheres, com idade de 18 a 35 anos (25,8 ± 6,06, com o ciclo menstrual regular (28 até 32 dias e que não faziam uso de anticoncepcional oral. Dados referentes ao ciclo menstrual e atividade física habitual foram obtidos através da aplicação de um questionário. A flexibilidade foi aferida através da goniometria, utilizando-se oito movimentos, em três fases do ciclo menstrual. Os sujeitos foram submetidos a um teste hormonal, em que foram verificadas as taxas de estrona, estradiol e progesterona. O tratamento estatístico foi feito através de análise descritiva e inferencial (teste de Kolmogorov-Smirnov e ANOVA. Os resultados encontrados demonstraram que não houve diferença significativa (p Este estudio ha tenido por objetivo investigar si existen diferencias en el grado de flexibilidad en las fases del ciclo menstrual de mujeres adultas jóvenes practicantes de gimnasia de academia. La muestra intencional fue compuesta de 20 mujeres, con edades entre 18 a 35 años (25,8 ± 6,06, con ciclo menstrual regular (28 a 32 días y que no hacían uso de anticonceptivo oral. Datos referentes al ciclo menstrual y actividad física habitual fueron obtenidos a través de una encuesta. La flexibilidad fue medida a través de la goniometría, utilizándose ocho movimientos, en tres fases del ciclo menstrual. Los individuos fueron sometidos a un test hormonal, en donde se verificaron la tasa de estrona, estradiol y progesterona. El tratamiento estadístico fue hecho a través del análisis descriptivo y de inferencia (test de Kolmogorov-Smirnov y ANOVA. Los resultados encontrados mostraron que no hubo diferencia significativa (p This study aims to verify if there are differences in the degree of flexibility in the phases of

  7. Protein sequence comparison and protein evolution

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, W.R. [Univ. of Virginia, Charlottesville, VA (United States). Dept. of Biochemistry

    1995-12-31

    This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. This tutorial examines how the information conserved during the evolution of a protein molecule can be used to infer reliably homology, and thus a shared proteinfold and possibly a shared active site or function. The authors start by reviewing a geological/evolutionary time scale. Next they look at the evolution of several protein families. During the tutorial, these families will be used to demonstrate that homologous protein ancestry can be inferred with confidence. They also examine different modes of protein evolution and consider some hypotheses that have been presented to explain the very earliest events in protein evolution. The next part of the tutorial will examine the technical aspects of protein sequence comparison. Both optimal and heuristic algorithms and their associated parameters that are used to characterize protein sequence similarities are discussed. Perhaps more importantly, they survey the statistics of local similarity scores, and how these statistics can both be used to improve the selectivity of a search and to evaluate the significance of a match. They them examine distantly related members of three protein families, the serine proteases, the glutathione transferases, and the G-protein-coupled receptors (GCRs). Finally, the discuss how sequence similarity can be used to examine internal repeated or mosaic structures in proteins.

  8. Inferring Protein Associations Using Protein Pulldown Assays

    Energy Technology Data Exchange (ETDEWEB)

    Sharp, Julia L.; Anderson, Kevin K.; Daly, Don S.; Auberry, Deanna L.; Borkowski, John J.; Cannon, William R.

    2007-02-01

    Background: One method to infer protein-protein associations is through a “bait-prey pulldown” assay using a protein affinity agent and an LC-MS (liquid chromatography-mass spectrometry)-based protein identification method. False positive and negative protein identifications are not uncommon, however, leading to incorrect inferences. Methods: A pulldown experiment generates a protein association matrix wherein each column represents a sample from one bait protein, each row represents one prey protein and each cell contains a presence/absence association indicator. Our method evaluates the presence/absence pattern across a prey protein (row) with a Likelihood Ratio Test (LRT), computing its p-value with simulated LRT test statistic distributions after a check with simulated binomial random variates disqualified the large sample 2 test. A pulldown experiment often involves hundreds of tests so we apply the false discovery rate method to control the false positive rate. Based on the p-value, each prey protein is assigned a category (specific association, non-specific association, or not associated) and appraised with respect to the pulldown experiment’s goal and design. The method is illustrated using a pulldown experiment investigating the protein complexes of Shewanella oneidensis MR-1. Results: The Monte Carlo simulated LRT p-values objectively reveal specific and ubiquitous prey, as well as potential systematic errors. The example analysis shows the results to be biologically sensible and more realistic than the ad hoc screening methods previously utilized. Conclusions: The method presented appears to be informative for screening for protein-protein associations.

  9. Prediction of Protein-Protein Interactions Using Protein Signature Profiling

    Institute of Scientific and Technical Information of China (English)

    Mahmood; A.; Mahdavi; Yen-Han; Lin

    2007-01-01

    Protein domains are conserved and functionally independent structures that play an important role in interactions among related proteins. Domain-domain inter- actions have been recently used to predict protein-protein interactions (PPI). In general, the interaction probability of a pair of domains is scored using a trained scoring function. Satisfying a threshold, the protein pairs carrying those domains are regarded as "interacting". In this study, the signature contents of proteins were utilized to predict PPI pairs in Saccharomyces cerevisiae, Caenorhabditis ele- gans, and Homo sapiens. Similarity between protein signature patterns was scored and PPI predictions were drawn based on the binary similarity scoring function. Results show that the true positive rate of prediction by the proposed approach is approximately 32% higher than that using the maximum likelihood estimation method when compared with a test set, resulting in 22% increase in the area un- der the receiver operating characteristic (ROC) curve. When proteins containing one or two signatures were removed, the sensitivity of the predicted PPI pairs in- creased significantly. The predicted PPI pairs are on average 11 times more likely to interact than the random selection at a confidence level of 0.95, and on aver- age 4 times better than those predicted by either phylogenetic profiling or gene expression profiling.

  10. Polymer Directed Protein Assemblies

    NARCIS (Netherlands)

    van Rijn, Patrick

    2013-01-01

    Protein aggregation and protein self-assembly is an important occurrence in natural systems, and is in some form or other dictated by biopolymers. Very obvious influences of biopolymers on protein assemblies are, e. g., virus particles. Viruses are a multi-protein assembly of which the morphology is

  11. Mirror image proteins.

    Science.gov (United States)

    Zhao, Le; Lu, Wuyuan

    2014-10-01

    Proteins composed entirely of unnatural d-amino acids and the achiral amino acid glycine are mirror image forms of their native l-protein counterparts. Recent advances in chemical protein synthesis afford unique and facile synthetic access to domain-sized mirror image d-proteins, enabling protein research to be conducted through 'the looking glass' and in a way previously unattainable. d-Proteins can facilitate structure determination of their native l-forms that are difficult to crystallize (racemic X-ray crystallography); d-proteins can serve as the bait for library screening to ultimately yield pharmacologically superior d-peptide/d-protein therapeutics (mirror-image phage display); d-proteins can also be used as a powerful mechanistic tool for probing molecular events in biology. This review examines recent progress in the application of mirror image proteins to structural biology, drug discovery, and immunology.

  12. InterProScan Result: FS871231 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS871231 FS871231_4_ORF2 6E31C3F25D2F3525 PANTHER PTHR12772 DNA REPLICATION COMPLEX... GINS PROTEIN PSF2 1.5e-61 T IPR007257 GINS complex, subunit Psf2 Cellular Component: nucleus (GO:0005634)|Biological Process: DNA replication (GO:0006260) ...

  13. InterProScan Result: BY944713 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BY944713 BY944713_4_ORF1 04B2570889809851 PANTHER PTHR12772 DNA REPLICATION COMPLEX... GINS PROTEIN PSF2 2.3e-05 T IPR007257 GINS complex, subunit Psf2 Cellular Component: nucleus (GO:0005634)|Biological Process: DNA replication (GO:0006260) ...

  14. InterProScan Result: FS852910 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS852910 FS852910_1_ORF2 6E31C3F25D2F3525 PANTHER PTHR12772 DNA REPLICATION COMPLEX... GINS PROTEIN PSF2 1.5e-61 T IPR007257 GINS complex, subunit Psf2 Cellular Component: nucleus (GO:0005634)|Biological Process: DNA replication (GO:0006260) ...

  15. Protein- protein interaction detection system using fluorescent protein microdomains

    Science.gov (United States)

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2010-02-23

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  16. [Protein expression and purification].

    Science.gov (United States)

    Růčková, E; Müller, P; Vojtěšek, B

    2014-01-01

    Production of recombinant proteins is essential for many applications in both basic research and also in medicine, where recombinant proteins are used as pharmaceuticals. This review summarizes procedures involved in recombinant protein expression and purification, including molecular cloning of target genes into expression vectors, selection of the appropriate expression system, and protein purification techniques. Recombinant DNA technology allows protein engineering to modify protein stability, activity and function or to facilitate protein purification by affinity tag fusions. A wide range of cloning systems enabling fast and effective design of expression vectors is currently available. A first choice of protein expression system is usually the bacteria Escherichia coli. The main advantages of this prokaryotic expression system are low cost and simplicity; on the other hand this system is often unsuitable for production of complex mammalian proteins. Protein expression mediated by eukaryotic cells (yeast, insect and mammalian cells) usually produces properly folded and posttranslationally modified proteins. How-ever, cultivation of insect and, especially, mammalian cells is time consuming and expensive. Affinity tagged recombinant proteins are purified efficiently using affinity chromatography. An affinity tag is a protein or peptide that mediates specific binding to a chromatography column, unbound proteins are removed during a washing step and pure protein is subsequently eluted. PMID:24945544

  17. Urine Protein and Urine Protein to Creatinine Ratio

    Science.gov (United States)

    ... limited. Home Visit Global Sites Search Help? Urine Protein and Urine Protein to Creatinine Ratio Share this page: Was this page helpful? Also known as: 24-Hour Urine Protein; Urine Total Protein; Urine Protein to Creatinine Ratio; ...

  18. IGSF9 Family Proteins

    DEFF Research Database (Denmark)

    Hansen, Maria; Walmod, Peter Schledermann

    2013-01-01

    The Drosophila protein Turtle and the vertebrate proteins immunoglobulin superfamily (IgSF), member 9 (IGSF9/Dasm1) and IGSF9B are members of an evolutionarily ancient protein family. A bioinformatics analysis of the protein family revealed that invertebrates contain only a single IGSF9 family gene......, the longest isoforms of the proteins have the same general organization as the neural cell adhesion molecule family of cell adhesion molecule proteins, and like this family of proteins, IGSF9 family members are expressed in the nervous system. A review of the literature revealed that Drosophila Turtle...... facilitates homophilic cell adhesion. Moreover, IGSF9 family proteins have been implicated in the outgrowth and branching of neurites, axon guidance, synapse maturation, self-avoidance, and tiling. However, despite the few published studies on IGSF9 family proteins, reports on the functions of both Turtle...

  19. Discover protein sequence signatures from protein-protein interaction data

    Directory of Open Access Journals (Sweden)

    Haasl Ryan J

    2005-11-01

    Full Text Available Abstract Background The development of high-throughput technologies such as yeast two-hybrid systems and mass spectrometry technologies has made it possible to generate large protein-protein interaction (PPI datasets. Mining these datasets for underlying biological knowledge has, however, remained a challenge. Results A total of 3108 sequence signatures were found, each of which was shared by a set of guest proteins interacting with one of 944 host proteins in Saccharomyces cerevisiae genome. Approximately 94% of these sequence signatures matched entries in InterPro member databases. We identified 84 distinct sequence signatures from the remaining 172 unknown signatures. The signature sharing information was then applied in predicting sub-cellular localization of yeast proteins and the novel signatures were used in identifying possible interacting sites. Conclusion We reported a method of PPI data mining that facilitated the discovery of novel sequence signatures using a large PPI dataset from S. cerevisiae genome as input. The fact that 94% of discovered signatures were known validated the ability of the approach to identify large numbers of signatures from PPI data. The significance of these discovered signatures was demonstrated by their application in predicting sub-cellular localizations and identifying potential interaction binding sites of yeast proteins.

  20. Protein and protein hydrolysates in sports nutrition.

    Science.gov (United States)

    van Loon, Luc J C; Kies, Arie K; Saris, Wim H M

    2007-08-01

    With the increasing knowledge about the role of nutrition in increasing exercise performance, it has become clear over the last 2 decades that amino acids, protein, and protein hydrolysates can play an important role. Most of the attention has been focused on their effects at a muscular level. As these nutrients are ingested, however, it also means that gastrointestinal digestibility and absorption can modulate their efficacy significantly. Therefore, discussing the role of amino acids, protein, and protein hydrolysates in sports nutrition entails holding a discussion on all levels of the metabolic route. On May 28-29, 2007, a small group of researchers active in the field of exercise science and protein metabolism presented an overview of the different aspects of the application of protein and protein hydrolysates in sports nutrition. In addition, they were asked to share their opinions on the future progress in their fields of research. In this overview, an introduction to the workshop and a short summary of its outcome is provided.

  1. Protein Data Bank (PDB)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Protein Data Bank (PDB) archive is the single worldwide repository of information about the 3D structures of large biological molecules, including proteins and...

  2. Learning about Proteins

    Science.gov (United States)

    ... need from peanuts alone, but if you have peanut butter on whole-grain bread, you're set. Likewise, ... protein in a day: 2 tablespoons (15 milliliters) peanut butter (7 grams protein) 1 cup (240 milliliters) low- ...

  3. Abnormal protein aggregationand neurodegenerativediseases

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Abnormal protein aggregation or amyloid is the major cause ofmany neurodegenerative disorders. The present review focuses on the correlation between sequence and structure features of proteins related to the diseases and abnormal protein aggregation. Recent progress has improved our knowledge on understand-ing the mechanism of amyloid formation. We suggest a nucleation model for ordered protein aggregation, which can also explain pathogenesis mechanisms of these neurodegenerative diseases in vivo.

  4. Destabilized bioluminescent proteins

    Science.gov (United States)

    Allen, Michael S.; Rakesh, Gupta; Gary, Sayler S.

    2007-07-31

    Purified nucleic acids, vectors and cells containing a gene cassette encoding at least one modified bioluminescent protein, wherein the modification includes the addition of a peptide sequence. The duration of bioluminescence emitted by the modified bioluminescent protein is shorter than the duration of bioluminescence emitted by an unmodified form of the bioluminescent protein.

  5. CSF total protein

    Science.gov (United States)

    CSF total protein is a test to determine the amount of protein in your spinal fluid, also called cerebrospinal fluid (CSF). ... The normal protein range varies from lab to lab, but is typically about 15 to 60 mg/dL. Note: mg/dL = ...

  6. Modeling Protein Domain Function

    Science.gov (United States)

    Baker, William P.; Jones, Carleton "Buck"; Hull, Elizabeth

    2007-01-01

    This simple but effective laboratory exercise helps students understand the concept of protein domain function. They use foam beads, Styrofoam craft balls, and pipe cleaners to explore how domains within protein active sites interact to form a functional protein. The activity allows students to gain content mastery and an understanding of the…

  7. Protein domain prediction

    NARCIS (Netherlands)

    Ingolfsson, Helgi; Yona, Golan

    2008-01-01

    Domains are considered to be the building blocks of protein structures. A protein can contain a single domain or multiple domains, each one typically associated with a specific function. The combination of domains determines the function of the protein, its subcellular localization and the interacti

  8. Protein - Which is Best?

    Science.gov (United States)

    Hoffman, Jay R; Falvo, Michael J

    2004-09-01

    Protein intake that exceeds the recommended daily allowance is widely accepted for both endurance and power athletes. However, considering the variety of proteins that are available much less is known concerning the benefits of consuming one protein versus another. The purpose of this paper is to identify and analyze key factors in order to make responsible recommendations to both the general and athletic populations. Evaluation of a protein is fundamental in determining its appropriateness in the human diet. Proteins that are of inferior content and digestibility are important to recognize and restrict or limit in the diet. Similarly, such knowledge will provide an ability to identify proteins that provide the greatest benefit and should be consumed. The various techniques utilized to rate protein will be discussed. Traditionally, sources of dietary protein are seen as either being of animal or vegetable origin. Animal sources provide a complete source of protein (i.e. containing all essential amino acids), whereas vegetable sources generally lack one or more of the essential amino acids. Animal sources of dietary protein, despite providing a complete protein and numerous vitamins and minerals, have some health professionals concerned about the amount of saturated fat common in these foods compared to vegetable sources. The advent of processing techniques has shifted some of this attention and ignited the sports supplement marketplace with derivative products such as whey, casein and soy. Individually, these products vary in quality and applicability to certain populations. The benefits that these particular proteins possess are discussed. In addition, the impact that elevated protein consumption has on health and safety issues (i.e. bone health, renal function) are also reviewed. Key PointsHigher protein needs are seen in athletic populations.Animal proteins is an important source of protein, however potential health concerns do exist from a diet of protein

  9. Desenvolvimento e validação de um novo sistema de seleção de talentos para a ginástica olímpica feminina: a Bateria PDGO Development and validation of a new system for talent selection in female artistic gymnastics: the PDGO Battery

    Directory of Open Access Journals (Sweden)

    Patrícia Arruda de Albuquerque

    2007-06-01

    Full Text Available A ginástica olímpica (GO envolve atividades motoras que exigem capacidades específicas. Todavia, há carência de testes para detectar o potencial para o desempenho na GO em idades precoces. Este estudo descreve e apresenta evidências da validade de uma bateria de testes para a seleção de talentos para GO feminina. Inicialmente, foram levantadas as atividades que vêm sendo aplicadas por especialistas em GO, na literatura e por consulta através de questionários. A partir das 30 atividades mencionadas, foi delineada uma bateria de testes com 22 atividades, aplicada em 55 crianças de cinco a nove anos de idade. Com uso de técnicas de análise fatorial, os escores fatoriais individuais foram calculados para o primeiro fator, sendo adotados como variável independente representativa do potencial das crianças para o desempenho em GO (PDGO. Uma regressão múltipla passo a passo gerou uma equação por meio da qual o PDGO era estimado a partir de apenas oito atividades. A validade cruzada da bateria e do índice PDGO foi verificada em 16 ginastas experientes e 19 crianças de escolinha. As primeiras obtiveram médias significativamente maiores (PDGO = 85,1 que as últimas (PDGO = 54,8, indicando que a bateria discriminou bem o potencial geral para o desempenho. Confirmou-se a validade construto com base na estrutura da análise fatorial, já que as atividades agruparam-se em torno de uma única dimensão do desempenho motor. Para a fidedignidade inter e intra-observadores, obtiveram-se correlações de 0,83 a 1,00, sem diferenças entre as médias nas situações observadas. Conclui-se que a bateria de testes, além de ser de aplicação rápida, simples, de baixo custo e fácil entendimento, reveste-se de validade e reprodutibilidade, podendo ser aplicada na seleção inicial de talentos para a GO. Estudos adicionais, porém, deveriam ser conduzidos para confirmação dos presentes resultados, envolvendo amostras maiores e abordagem

  10. Relação entre os achados audiométricos e as queixas auditivas e extra-auditivas dos professores de uma academia de ginástica Relationship between audiometric findings and auditory and extra-auditory complaints of physical education teachers

    Directory of Open Access Journals (Sweden)

    Isabela Freixo Côrtes de Andrade

    2010-01-01

    Full Text Available OBJETIVOS: Estudar as queixas auditivas e extra-auditivas de professores de uma academia de ginástica expostos à música eletronicamente amplificada, descrever os achados audiométricos e investigar a influência das variáveis, tempo de profissão, carga horária de trabalho e presença de entalhe audiométrico. MÉTODOS: Inicialmente, foi realizada a medida dos níveis de pressão sonora das seis salas de aula da academia (média 101,4 dB(A. Em seguida, 32 professores foram avaliados por meio de anamnese e pesquisa dos limiares tonais por via aérea, e a presença de entalhe audiométrico foi investigada. RESULTADOS: Foi encontrada uma elevada porcentagem de professores (87,5% com exposições extra-ocupacionais. As queixas mais frequentes foram: intolerância aos sons intensos (43,8%, tontura (12,5%; irritabilidade e nervosismo (28,1%; insônia (31,3%; dor de cabeça (37,5%; zumbido (12,5%; plenitude auricular (12,5% e dificuldade de escutar às vezes (43,8%. Em relação aos achados audiométricos, 9,37% apresentaram alteração: 3,12% por perda neurossensorial bilateral e 6,25% queda nas frequências de 3000 Hz ou 4000 Hz. A frequência mais acometida pelo entalhe audiométrico foi 6000 Hz, sendo que 50% o apresentaram em, pelo menos, uma orelha. Não houve diferença estatisticamente significante na comparação entre o tempo de profissão, horas trabalhadas e queixas com os resultados do entalhe audiométrico. CONCLUSÃO: 68,75% dos professores apresentaram queixas extra-auditivas. Os resultados mostraram que apesar de ocorrer uma porcentagem pequena (9,37% de perda auditiva na população estudada, já existe na metade dos professores entalhe audiométrico em pelo menos uma orelha.PURPOSE: To study auditory and extra-auditory complaints of physical education teachers exposed to electronically amplified music, to describe their audiometric findings, and to investigate the importance of the variables time of profession, work-journey, and

  11. Protopia: a protein-protein interaction tool

    Science.gov (United States)

    Real-Chicharro, Alejandro; Ruiz-Mostazo, Iván; Navas-Delgado, Ismael; Kerzazi, Amine; Chniber, Othmane; Sánchez-Jiménez, Francisca; Medina, Miguel Ángel; Aldana-Montes, José F

    2009-01-01

    Background Protein-protein interactions can be considered the basic skeleton for living organism self-organization and homeostasis. Impressive quantities of experimental data are being obtained and computational tools are essential to integrate and to organize this information. This paper presents Protopia, a biological tool that offers a way of searching for proteins and their interactions in different Protein Interaction Web Databases, as a part of a multidisciplinary initiative of our institution for the integration of biological data . Results The tool accesses the different Databases (at present, the free version of Transfac, DIP, Hprd, Int-Act and iHop), and results are expressed with biological protein names or databases codes and can be depicted as a vector or a matrix. They can be represented and handled interactively as an organic graph. Comparison among databases is carried out using the Uniprot codes annotated for each protein. Conclusion The tool locates and integrates the current information stored in the aforementioned databases, and redundancies among them are detected. Results are compatible with the most important network analysers, so that they can be compared and analysed by other world-wide known tools and platforms. The visualization possibilities help to attain this goal and they are especially interesting for handling multiple-step or complex networks. PMID:19828077

  12. Protein hydration and dynamics

    International Nuclear Information System (INIS)

    Inelastic neutron scattering can measure the protein thermal fluctuations under the physiological aqueous environment, especially it is powerful to observe the low-energy protein dynamics in THz region, which are revealed theoretically to be coupled with solvations. Neutron enables the selective observation of protein and hydration water by deuteration. The complementary analysis with molecular dynamics simulation is also effective for the study of protein hydration. Some examples of the application toward the understanding of molecular basis of protein functions will be introduced. (author)

  13. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  14. Racemic protein crystallography.

    Science.gov (United States)

    Yeates, Todd O; Kent, Stephen B H

    2012-01-01

    Although natural proteins are chiral and are all of one "handedness," their mirror image forms can be prepared by chemical synthesis. This opens up new opportunities for protein crystallography. A racemic mixture of the enantiomeric forms of a protein molecule can crystallize in ways that natural proteins cannot. Recent experimental data support a theoretical prediction that this should make racemic protein mixtures highly amenable to crystallization. Crystals obtained from racemic mixtures also offer advantages in structure determination strategies. The relevance of these potential advantages is heightened by advances in synthetic methods, which are extending the size limit for proteins that can be prepared by chemical synthesis. Recent ideas and results in the area of racemic protein crystallography are reviewed.

  15. Protein kinesis: The dynamics of protein trafficking and stability

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-31

    The purpose of this conference is to provide a multidisciplinary forum for exchange of state-of-the-art information on protein kinesis. This volume contains abstracts of papers in the following areas: protein folding and modification in the endoplasmic reticulum; protein trafficking; protein translocation and folding; protein degradation; polarity; nuclear trafficking; membrane dynamics; and protein import into organelles.

  16. Protein: FBA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA3 Atg1 kinase complex ATG1 APG1, AUT3, CVT10 Serine/threonine-protein kinase ATG1 Autophagy protein... 3, Autophagy-related protein 1, Cytoplasm to vacuole targeting protein 10 559292 Sacchar

  17. Protein: FEA4 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEA4 Proteins in gibberellin signaling GID2 F-box protein GID2 Gibberellin-insensitive dwarf protein... 2, Protein GIBBERELLIN INSENSITIVE DWARF2 39947 Oryza sativa subsp. japonica Q7XAK4 ...

  18. Protein Electrophoresis/Immunofixation Electrophoresis

    Science.gov (United States)

    ... be limited. Home Visit Global Sites Search Help? Protein Electrophoresis Immunofixation Electrophoresis Share this page: Was this page helpful? Also known as: Serum Protein Electrophoresis; Protein ELP; SPE; SPEP; Urine Protein Electrophoresis; ...

  19. NMR of unfolded proteins

    Indian Academy of Sciences (India)

    Amarnath Chtterjee; Ashutosh Kumar; Jeetender Chugh; Sudha Srivastava; Neel S Bhavesh; Ramakrishna V Hosur

    2005-01-01

    In the post-genomic era, as more and more genome sequences are becoming known and hectic efforts are underway to decode the information content in them, it is becoming increasingly evident that flexibility in proteins plays a crucial role in many of the biological functions. Many proteins have intrinsic disorder either wholly or in specific regions. It appears that this disorder may be important for regulatory functions of the proteins, on the one hand, and may help in directing the folding process to reach the compact native state, on the other. Nuclear magnetic resonance (NMR) has over the last two decades emerged as the sole, most powerful technique to help characterize these disordered protein systems. In this review, we first discuss the significance of disorder in proteins and then describe the recent developments in NMR methods for their characterization. A brief description of the results obtained on several disordered proteins is presented at the end.

  20. Computational Protein Design

    DEFF Research Database (Denmark)

    Johansson, Kristoffer Enøe

    Proteins are the major functional group of molecules in biology. The impact of protein science on medicine and chemical productions is rapidly increasing. However, the greatest potential remains to be realized. The fi eld of protein design has advanced computational modeling from a tool of support...... to a central method that enables new developments. For example, novel enzymes with functions not found in natural proteins have been de novo designed to give enough activity for experimental optimization. This thesis presents the current state-of-the-art within computational design methods together...... with a novel method based on probability theory. With the aim of assembling a complete pipeline for protein design, this work touches upon several aspects of protein design. The presented work is the computational half of a design project where the other half is dedicated to the experimental part...

  1. Staining Proteins in Gels

    OpenAIRE

    Gallagher, Sean; Chakavarti, Deb

    2008-01-01

    Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for detecting proteins by four popular methods. Coomassie blue staining is an easy and rapid method. Silver staining, while more time consuming, is considerably more sensitive and can thus be used to detect smaller amounts of protein. Fluorescent staining is a popular alternative to traditional staining procedures, mainly because it is more sensitive th...

  2. Pressure cryocooling protein crystals

    Science.gov (United States)

    Kim, Chae Un; Gruner, Sol M.

    2011-10-04

    Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.

  3. Proteins at interfaces

    OpenAIRE

    Evers, Florian

    2011-01-01

    Protein adsorption is a fundamental and ubiquitous phenomenon, which has severe implications in the fields of biomaterials as well as bio- and nanotechnology, e.g., in drug delivery, biofouling, the biocompatibility of implants, food chemistry, and biosensors. Therefore, the mechanisms of protein adsorption and controlling the interfacial affinity of proteins have become intriguing and interdisciplinary research topics. In this work, X-ray and neutron reflectometry are the main...

  4. Acanthamoeba castellanii STAT protein.

    Directory of Open Access Journals (Sweden)

    Anna Kicinska

    Full Text Available STAT (signal transducers and activators of transcription proteins are one of the important mediators of phosphotyrosine-regulated signaling in metazoan cells. We described the presence of STAT protein in a unicellular, free-living amoebae with a simple life cycle, Acanthamoeba castellanii. A. castellanii is the only, studied to date, Amoebozoan that does not belong to Mycetozoa but possesses STATs. A sequence of the A. castellanii STAT protein includes domains similar to those of the Dictyostelium STAT proteins: a coiled coil (characteristic for Dictyostelium STAT coiled coil, a STAT DNA-binding domain and a Src-homology domain. The search for protein sequences homologous to A. castellanii STAT revealed 17 additional sequences from lower eukaryotes. Interestingly, all of these sequences come from Amoebozoa organisms that belong to either Mycetozoa (slime molds or Centramoebida. We showed that there are four separated clades within the slime mold STAT proteins. The A. castellanii STAT protein branches next to a group of STATc proteins from Mycetozoa. We also demonstrate that Amoebozoa form a distinct monophyletic lineage within the STAT protein world that is well separated from the other groups.

  5. Acanthamoeba castellanii STAT protein.

    Science.gov (United States)

    Kicinska, Anna; Leluk, Jacek; Jarmuszkiewicz, Wieslawa

    2014-01-01

    STAT (signal transducers and activators of transcription) proteins are one of the important mediators of phosphotyrosine-regulated signaling in metazoan cells. We described the presence of STAT protein in a unicellular, free-living amoebae with a simple life cycle, Acanthamoeba castellanii. A. castellanii is the only, studied to date, Amoebozoan that does not belong to Mycetozoa but possesses STATs. A sequence of the A. castellanii STAT protein includes domains similar to those of the Dictyostelium STAT proteins: a coiled coil (characteristic for Dictyostelium STAT coiled coil), a STAT DNA-binding domain and a Src-homology domain. The search for protein sequences homologous to A. castellanii STAT revealed 17 additional sequences from lower eukaryotes. Interestingly, all of these sequences come from Amoebozoa organisms that belong to either Mycetozoa (slime molds) or Centramoebida. We showed that there are four separated clades within the slime mold STAT proteins. The A. castellanii STAT protein branches next to a group of STATc proteins from Mycetozoa. We also demonstrate that Amoebozoa form a distinct monophyletic lineage within the STAT protein world that is well separated from the other groups. PMID:25338074

  6. Moonlighting proteins in cancer.

    Science.gov (United States)

    Min, Kyung-Won; Lee, Seong-Ho; Baek, Seung Joon

    2016-01-01

    Since the 1980s, growing evidence suggested that the cellular localization of proteins determined their activity and biological functions. In a classical view, a protein is characterized by the single cellular compartment where it primarily resides and functions. It is now believed that when proteins appear in different subcellular locations, the cells surpass the expected activity of proteins given the same genomic information to fulfill complex biological behavior. Many proteins are recognized for having the potential to exist in multiple locations in cells. Dysregulation of translocation may cause cancer or contribute to poorer cancer prognosis. Thus, quantitative and comprehensive assessment of dynamic proteins and associated protein movements could be a promising indicator in determining cancer prognosis and efficiency of cancer treatment and therapy. This review will summarize these so-called moonlighting proteins, in terms of a coupled intracellular cancer signaling pathway. Determination of the detailed biological intracellular and extracellular transit and regulatory activity of moonlighting proteins permits a better understanding of cancer and identification of potential means of molecular intervention.

  7. Engineering therapeutic protein disaggregases

    Science.gov (United States)

    Shorter, James

    2016-01-01

    Therapeutic agents are urgently required to cure several common and fatal neurodegenerative disorders caused by protein misfolding and aggregation, including amyotrophic lateral sclerosis (ALS), Parkinson’s disease (PD), and Alzheimer’s disease (AD). Protein disaggregases that reverse protein misfolding and restore proteins to native structure, function, and localization could mitigate neurodegeneration by simultaneously reversing 1) any toxic gain of function of the misfolded form and 2) any loss of function due to misfolding. Potentiated variants of Hsp104, a hexameric AAA+ ATPase and protein disaggregase from yeast, have been engineered to robustly disaggregate misfolded proteins connected with ALS (e.g., TDP-43 and FUS) and PD (e.g., α-synuclein). However, Hsp104 has no metazoan homologue. Metazoa possess protein disaggregase systems distinct from Hsp104, including Hsp110, Hsp70, and Hsp40, as well as HtrA1, which might be harnessed to reverse deleterious protein misfolding. Nevertheless, vicissitudes of aging, environment, or genetics conspire to negate these disaggregase systems in neurodegenerative disease. Thus, engineering potentiated human protein disaggregases or isolating small-molecule enhancers of their activity could yield transformative therapeutics for ALS, PD, and AD. PMID:27255695

  8. MicroProteins

    DEFF Research Database (Denmark)

    Eguen, Teinai Ebimienere; Straub, Daniel; Graeff, Moritz;

    2015-01-01

    MicroProteins (miPs) are short, usually single-domain proteins that, in analogy to miRNAs, heterodimerize with their targets and exert a dominant-negative effect. Recent bioinformatic attempts to identify miPs have resulted in a list of potential miPs, many of which lack the defining characterist......MicroProteins (miPs) are short, usually single-domain proteins that, in analogy to miRNAs, heterodimerize with their targets and exert a dominant-negative effect. Recent bioinformatic attempts to identify miPs have resulted in a list of potential miPs, many of which lack the defining...

  9. How Many Protein-Protein Interactions Types Exist in Nature?

    OpenAIRE

    Leonardo Garma; Srayanta Mukherjee; Pralay Mitra; Yang Zhang

    2012-01-01

    "Protein quaternary structure universe" refers to the ensemble of all protein-protein complexes across all organisms in nature. The number of quaternary folds thus corresponds to the number of ways proteins physically interact with other proteins. This study focuses on answering two basic questions: Whether the number of protein-protein interactions is limited and, if yes, how many different quaternary folds exist in nature. By all-to-all sequence and structure comparisons, we grouped the pro...

  10. Poxviral Ankyrin Proteins

    Directory of Open Access Journals (Sweden)

    Michael H. Herbert

    2015-02-01

    Full Text Available Multiple repeats of the ankyrin motif (ANK are ubiquitous throughout the kingdoms of life but are absent from most viruses. The main exception to this is the poxvirus family, and specifically the chordopoxviruses, with ANK repeat proteins present in all but three species from separate genera. The poxviral ANK repeat proteins belong to distinct orthologue groups spread over different species, and align well with the phylogeny of their genera. This distribution throughout the chordopoxviruses indicates these proteins were present in an ancestral vertebrate poxvirus, and have since undergone numerous duplication events. Most poxviral ANK repeat proteins contain an unusual topology of multiple ANK motifs starting at the N-terminus with a C-terminal poxviral homologue of the cellular F-box enabling interaction with the cellular SCF ubiquitin ligase complex. The subtle variations between ANK repeat proteins of individual poxviruses suggest an array of different substrates may be bound by these protein-protein interaction domains and, via the F-box, potentially directed to cellular ubiquitination pathways and possible degradation. Known interaction partners of several of these proteins indicate that the NF-κB coordinated anti-viral response is a key target, whilst some poxviral ANK repeat domains also have an F-box independent affect on viral host-range.

  11. Brushes and proteins

    NARCIS (Netherlands)

    Bosker, W.T.E.

    2011-01-01

      Brushes and Proteins   Wouter T. E. Bosker         Protein adsorption at solid surfaces can be prevented by applying a polymer brush at the surface. A polymer brush consists of polymer chains end-grafted to the surface at such a grafting density that th

  12. Proteins in biomass streams

    NARCIS (Netherlands)

    Mulder, W.J.

    2010-01-01

    The focus of this study is to give an overview of traditional and new biomasses and biomass streams that contain proteins. When information was available, the differences in molecular structure and physical and chemical properties for the different proteins is given. For optimal biomass use, isolati

  13. Protein Attachment on Nanodiamonds.

    Science.gov (United States)

    Lin, Chung-Lun; Lin, Cheng-Huang; Chang, Huan-Cheng; Su, Meng-Chih

    2015-07-16

    A recent advance in nanotechnology is the scale-up production of small and nonaggregated diamond nanoparticles suitable for biological applications. Using detonation nanodiamonds (NDs) with an average diameter of ∼4 nm as the adsorbents, we have studied the static attachment of three proteins (myoglobin, bovine serum albumin, and insulin) onto the nanoparticles by optical spectroscopy, mass spectrometry, and dynamic light scattering, and electrophoretic zeta potential measurements. Results show that the protein surface coverage is predominantly determined by the competition between protein-protein and protein-ND interactions, giving each protein a unique and characteristic structural configuration in its own complex. Specifically, both myoglobin and bovine serum albumin show a Langmuir-type adsorption behavior, forming 1:1 complexes at saturation, whereas insulin folds into a tightly bound multimer before adsorption. The markedly different adsorption patterns appear to be independent of the protein concentration and are closely related to the affinity of the individual proteins for the NDs. The present study provides a fundamental understanding for the use of NDs as a platform for nanomedical drug delivery. PMID:25815400

  14. Protein Unfolding and Alzheimer's

    Science.gov (United States)

    Cheng, Kelvin

    2012-10-01

    Early interaction events of beta-amyloid (Aβ) proteins with neurons have been associated with the pathogenesis of Alzheimer's disease. Knowledge pertaining to the role of lipid molecules, particularly cholesterol, in modulating the single Aβ interactions with neurons at the atomic length and picosecond time resolutions, remains unclear. In our research, we have used atomistic molecular dynamics simulations to explore early molecular events including protein insertion kinetics, protein unfolding, and protein-induced membrane disruption of Aβ in lipid domains that mimic the nanoscopic raft and non-raft regions of the neural membrane. In this talk, I will summarize our current work on investigating the role of cholesterol in regulating the Aβ interaction events with membranes at the molecular level. I will also explain how our results will provide new insights into understanding the pathogenesis of Alzheimer's disease associated with the Aβ proteins.

  15. Sensitizing properties of proteins

    DEFF Research Database (Denmark)

    Poulsen, Lars K.; Ladics, Gregory S; McClain, Scott;

    2014-01-01

    The scope of allergy risk is diverse considering the myriad ways in which protein allergenicity is affected by physiochemical characteristics of proteins. The complexity created by the matrices of foods and the variability of the human immune system add additional challenges to understanding...... the relationship between sensitization potential and allergy disease. To address these and other issues, an April 2012 international symposium was held in Prague, Czech Republic, to review and discuss the state-of-the-science of sensitizing properties of protein allergens. The symposium, organized by the Protein...... Allergenicity Technical Committee of the International Life Sciences Institute's Health and Environmental Sciences Institute, featured presentations on current methods, test systems, research trends, and unanswered questions in the field of protein sensitization. A diverse group of over 70 interdisciplinary...

  16. Bacterial Ice Crystal Controlling Proteins

    OpenAIRE

    Lorv, Janet S. H.; Rose, David R; Glick, Bernard R.

    2014-01-01

    Across the world, many ice active bacteria utilize ice crystal controlling proteins for aid in freezing tolerance at subzero temperatures. Ice crystal controlling proteins include both antifreeze and ice nucleation proteins. Antifreeze proteins minimize freezing damage by inhibiting growth of large ice crystals, while ice nucleation proteins induce formation of embryonic ice crystals. Although both protein classes have differing functions, these proteins use the same ice binding mechanisms. R...

  17. The centrality of cancer proteins in human protein-protein interaction network: a revisit.

    Science.gov (United States)

    Xiong, Wei; Xie, Luyu; Zhou, Shuigeng; Liu, Hui; Guan, Jihong

    2014-01-01

    Topological analysis of protein-protein interaction (PPI) networks has been widely applied to the investigation on cancer mechanisms. However, there is still a debate on whether cancer proteins exhibit more topological centrality compared to the other proteins in the human PPI network. To resolve this debate, we first identified four sets of human proteins, and then mapped these proteins into the yeast PPI network by homologous genes. Finally, we compared these proteins' properties in human and yeast PPI networks. Experiments over two real datasets demonstrated that cancer proteins tend to have higher degree and smaller clustering coefficient than non-cancer proteins. Experimental results also validated that cancer proteins have larger betweenness centrality compared to the other proteins on the STRING dataset. However, on the BioGRID dataset, the average betweenness centrality of cancer proteins is larger than that of disease and control proteins, but smaller than that of essential proteins. PMID:24878726

  18. PSC: protein surface classification.

    Science.gov (United States)

    Tseng, Yan Yuan; Li, Wen-Hsiung

    2012-07-01

    We recently proposed to classify proteins by their functional surfaces. Using the structural attributes of functional surfaces, we inferred the pairwise relationships of proteins and constructed an expandable database of protein surface classification (PSC). As the functional surface(s) of a protein is the local region where the protein performs its function, our classification may reflect the functional relationships among proteins. Currently, PSC contains a library of 1974 surface types that include 25,857 functional surfaces identified from 24,170 bound structures. The search tool in PSC empowers users to explore related surfaces that share similar local structures and core functions. Each functional surface is characterized by structural attributes, which are geometric, physicochemical or evolutionary features. The attributes have been normalized as descriptors and integrated to produce a profile for each functional surface in PSC. In addition, binding ligands are recorded for comparisons among homologs. PSC allows users to exploit related binding surfaces to reveal the changes in functionally important residues on homologs that have led to functional divergence during evolution. The substitutions at the key residues of a spatial pattern may determine the functional evolution of a protein. In PSC (http://pocket.uchicago.edu/psc/), a pool of changes in residues on similar functional surfaces is provided.

  19. Protein oxidation in aquatic foods

    DEFF Research Database (Denmark)

    Baron, Caroline P.

    2014-01-01

    The chapter discusses general considerations about protein oxidation and reviews the mechanisms involved in protein oxidation and consequences of protein oxidation on fish proteins. It presents two case studies, the first deals with protein and lipid oxidation in frozen rainbow trout......, and the second with oxidation in salted herring. The mechanisms responsible for initiation of protein oxidation are unclear, but it is generally accepted that free radical species initiating lipid oxidation can also initiate protein oxidation. The chapter focuses on interaction between protein and lipid...... oxidation. The protein carbonyl group measurement is the widely used method for estimating protein oxidation in foods and has been used in fish muscle. The chapter also talks about the impact of protein oxidation on protein functionality, fish muscle texture, and food nutritional value. Protein oxidation...

  20. Bacterial ice crystal controlling proteins.

    Science.gov (United States)

    Lorv, Janet S H; Rose, David R; Glick, Bernard R

    2014-01-01

    Across the world, many ice active bacteria utilize ice crystal controlling proteins for aid in freezing tolerance at subzero temperatures. Ice crystal controlling proteins include both antifreeze and ice nucleation proteins. Antifreeze proteins minimize freezing damage by inhibiting growth of large ice crystals, while ice nucleation proteins induce formation of embryonic ice crystals. Although both protein classes have differing functions, these proteins use the same ice binding mechanisms. Rather than direct binding, it is probable that these protein classes create an ice surface prior to ice crystal surface adsorption. Function is differentiated by molecular size of the protein. This paper reviews the similar and different aspects of bacterial antifreeze and ice nucleation proteins, the role of these proteins in freezing tolerance, prevalence of these proteins in psychrophiles, and current mechanisms of protein-ice interactions. PMID:24579057

  1. Anchored design of protein-protein interfaces.

    Directory of Open Access Journals (Sweden)

    Steven M Lewis

    Full Text Available BACKGROUND: Few existing protein-protein interface design methods allow for extensive backbone rearrangements during the design process. There is also a dichotomy between redesign methods, which take advantage of the native interface, and de novo methods, which produce novel binders. METHODOLOGY: Here, we propose a new method for designing novel protein reagents that combines advantages of redesign and de novo methods and allows for extensive backbone motion. This method requires a bound structure of a target and one of its natural binding partners. A key interaction in this interface, the anchor, is computationally grafted out of the partner and into a surface loop on the design scaffold. The design scaffold's surface is then redesigned with backbone flexibility to create a new binding partner for the target. Careful choice of a scaffold will bring experimentally desirable characteristics into the new complex. The use of an anchor both expedites the design process and ensures that binding proceeds against a known location on the target. The use of surface loops on the scaffold allows for flexible-backbone redesign to properly search conformational space. CONCLUSIONS AND SIGNIFICANCE: This protocol was implemented within the Rosetta3 software suite. To demonstrate and evaluate this protocol, we have developed a benchmarking set of structures from the PDB with loop-mediated interfaces. This protocol can recover the correct loop-mediated interface in 15 out of 16 tested structures, using only a single residue as an anchor.

  2. An Algorithm for Finding Functional Modules and Protein Complexes in Protein-Protein Interaction Networks

    OpenAIRE

    Guangyu Cui; Yu Chen; De-Shuang Huang; Kyungsook Han

    2008-01-01

    Biological processes are often performed by a group of proteins rather than by individual proteins, and proteins in a same biological group form a densely connected subgraph in a protein-protein interaction network. Therefore, finding a densely connected subgraph provides useful information to predict the function or protein complex of uncharacterized proteins in the highly connected subgraph. We have developed an efficient algorithm and program for finding cliques and near-cliques in a prote...

  3. Quantification of the Influence of Protein-Protein Interactions on Adsorbed Protein Structure and Bioactivity

    OpenAIRE

    Wei, Yang; Thyparambil, Aby A.; Latour, Robert A.

    2013-01-01

    While protein-surface interactions have been widely studied, relatively little is understood at this time regarding how protein-surface interaction effects are influenced by protein-protein interactions and how these effects combine with the internal stability of a protein to influence its adsorbed-state structure and bioactivity. The objectives of this study were to develop a method to study these combined effects under widely varying protein-protein interaction conditions using hen egg-whit...

  4. Protein: FEB6 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEB6 Photoresponse regulatory proteins HD1 SE1 Zinc finger protein HD1 Protein CONSTANS-like, Protein... HEADING DATE 1, Protein PHOTOPERIOD SENSITIVITY 1 39947 Oryza sativa subsp. japonica 4340746 Q9FDX8 21952207, 19246394 #shimamoto ...

  5. Protein: MPA6 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available in 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q ...and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein 9606 Homo sapiens Q15848 9370 9370 Q15848 18054335, 19646806 ...

  6. Protein crystallography prescreen kit

    Science.gov (United States)

    Segelke, Brent W.; Krupka, Heike I.; Rupp, Bernhard

    2005-07-12

    A kit for prescreening protein concentration for crystallization includes a multiplicity of vials, a multiplicity of pre-selected reagents, and a multiplicity of sample plates. The reagents and a corresponding multiplicity of samples of the protein in solutions of varying concentrations are placed on sample plates. The sample plates containing the reagents and samples are incubated. After incubation the sample plates are examined to determine which of the sample concentrations are too low and which the sample concentrations are too high. The sample concentrations that are optimal for protein crystallization are selected and used.

  7. Human Protein Z.

    OpenAIRE

    Broze, G J; Miletich, J P

    1984-01-01

    Protein Z was purified from human plasma by a four-step procedure which included barium citrate adsorption, ammonium sulfate fractionation, DEAE-Sepharose chromatography, and blue agarose chromatography with a yield of 20%. It is a 62,000 mol wt protein with an extinction coefficient of 12.0. The concentration of Protein Z in pooled, citrated plasma is 2.2 micrograms/ml and its half-life in patients starting warfarin anticoagulation therapy is estimated to be less than 2.5 d. The NH2-terminal...

  8. Piezoelectric allostery of protein.

    Science.gov (United States)

    Ohnuki, Jun; Sato, Takato; Takano, Mitsunori

    2016-07-01

    Allostery is indispensable for a protein to work, where a locally applied stimulus is transmitted to a distant part of the molecule. While the allostery due to chemical stimuli such as ligand binding has long been studied, the growing interest in mechanobiology prompts the study of the mechanically stimulated allostery, the physical mechanism of which has not been established. By molecular dynamics simulation of a motor protein myosin, we found that a locally applied mechanical stimulus induces electrostatic potential change at distant regions, just like the piezoelectricity. This novel allosteric mechanism, "piezoelectric allostery", should be of particularly high value for mechanosensor/transducer proteins. PMID:27575163

  9. Evolution of proteins.

    Science.gov (United States)

    Dayhoff, M. O.

    1971-01-01

    The amino acid sequences of proteins from living organisms are dealt with. The structure of proteins is first discussed; the variation in this structure from one biological group to another is illustrated by the first halves of the sequences of cytochrome c, and a phylogenetic tree is derived from the cytochrome c data. The relative geological times associated with the events of this tree are discussed. Errors which occur in the duplication of cells during the evolutionary process are examined. Particular attention is given to evolution of mutant proteins, globins, ferredoxin, and transfer ribonucleic acids (tRNA's). Finally, a general outline of biological evolution is presented.

  10. Protein oxidation and ageing

    DEFF Research Database (Denmark)

    Linton, S; Davies, Michael Jonathan; Dean, R T

    2001-01-01

    of redox-active metal ions that could catalyse oxidant formation. As a result of this decrease in antioxidant defences, and increased rate of ROS formation, it is possible that the impact of ROS increases with age. ROS are known to oxidise biological macromolecules, with proteins an important target....... If the argument that the impact of ROS increases with age is true, then proteins would be expected to accumulate oxidised materials with age, and the rate of such accumulation should increase with time, reflecting impaired inefficiency of homeostasis. Here we review the evidence for the accumulation of oxidised......, or modified, extra- and intra-cellular proteins in vivo....

  11. Sound of proteins

    DEFF Research Database (Denmark)

    2007-01-01

    In my group we work with Molecular Dynamics to model several different proteins and protein systems. We submit our modelled molecules to changes in temperature, changes in solvent composition and even external pulling forces. To analyze our simulation results we have so far used visual inspection...... and statistical analysis of the resulting molecular trajectories (as everybody else!). However, recently I started assigning a particular sound frequency to each amino acid in the protein, and by setting the amplitude of each frequency according to the movement amplitude we can "hear" whenever two aminoacids...

  12. Alpha Shapes and Proteins

    DEFF Research Database (Denmark)

    Winter, Pawel; Sterner, Henrik; Sterner, Peter

    2009-01-01

    We provide a unified description of (weighted) alpha shapes, beta shapes and the corresponding simplicialcomplexes. We discuss their applicability to various protein-related problems. We also discuss filtrations of alpha shapes and touch upon related persistence issues.We claim that the full...... potential of alpha-shapes and related geometrical constructs in protein-related problems yet remains to be realized and verified. We suggest parallel algorithms for (weighted) alpha shapes, and we argue that future use of filtrations and kinetic variants for larger proteins will need such implementation....

  13. New approach for predicting protein-protein interactions

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Protein-protein interactions (PPIs) are of vital importance for virtually all processes of a living cell. The study of these associations of protein molecules could improve people's understanding of diseases and provide basis for therapeutic approaches.

  14. Analysis of correlations between protein complex and protein-protein interaction and mRNA expression

    Institute of Scientific and Technical Information of China (English)

    CAI Lun; XUE Hong; LU Hongchao; ZHAO Yi; ZHU Xiaopeng; BU Dongbo; LING Lunjiang; CHEN Runsheng

    2003-01-01

    Protein-protein interaction is a physical interaction of two proteins in living cells. In budding yeast Saccharomyces cerevisiae, large-scale protein-protein interaction data have been obtained through high-throughput yeast two-hybrid systems (Y2H) and protein complex purification techniques based on mass-spectrometry. Here, we collect 11855 interactions between total 2617 proteins. Through seriate genome-wide mRNA expression data, similarity between two genes could be measured. Protein complex data can also be obtained publicly and can be translated to pair relationship that any two proteins can only exist in the same complex or not. Analysis of protein complex data, protein-protein interaction data and mRNA expression data can elucidate correlations between them. The results show that proteins that have interactions or similar expression patterns have a higher possibility to be in the same protein complex than randomized selected proteins, and proteins which have interactions and similar expression patterns are even more possible to exist in the same protein complex. The work indicates that comprehensive integration and analysis of public large-scale bioinformatical data, such as protein complex data, protein-protein interaction data and mRNA expression data, may help to uncover their relationships and common biological information underlying these data. The strategies described here may help to integrate and analyze other functional genomic and proteomic data, such as gene expression profiling, protein-localization mapping and large-scale phenotypic data, both in yeast and in other organisms.

  15. A Bayesian Estimator of Protein-Protein Association Probabilities

    Energy Technology Data Exchange (ETDEWEB)

    Gilmore, Jason M.; Auberry, Deanna L.; Sharp, Julia L.; White, Amanda M.; Anderson, Kevin K.; Daly, Don S.

    2008-07-01

    The Bayesian Estimator of Protein-Protein Association Probabilities (BEPro3) is a software tool for estimating probabilities of protein-protein association between bait and prey protein pairs using data from multiple-bait, multiple-replicate, protein pull-down LC-MS assay experiments. BEPro3 is open source software that runs on both Windows XP and Mac OS 10.4 or newer versions, and is freely available from http://www.pnl.gov/statistics/BEPro3.

  16. Whey Protein- The Role of Protein Supplementation in Resistance Training

    OpenAIRE

    Zimmer, Raymond

    2005-01-01

    Adequate protein intake is an important concern for many athletes who are undergoing strength-training programs. Many athletes choose to take a protein supplement, such as whey protein, in order to help them build lean muscle mass more efficiently. But the benefit of very high levels of dietary protein in resistance training remains questionable. This paper examines the effectiveness of whey protein, and other forms of protein supplements, in helping athletes augment their muscle mass. A comp...

  17. Protein-protein interaction databases: keeping up with growing interactomes

    OpenAIRE

    Lehne Benjamin; Schlitt Thomas

    2009-01-01

    Abstract Over the past few years, the number of known protein-protein interactions has increased substantially. To make this information more readily available, a number of publicly available databases have set out to collect and store protein-protein interaction data. Protein-protein interactions have been retrieved from six major databases, integrated and the results compared. The six databases (the Biological General Repository for Interaction Datasets [BioGRID], the Molecular INTeraction ...

  18. Interactive protein manipulation

    Energy Technology Data Exchange (ETDEWEB)

    SNCrivelli@lbl.gov

    2003-07-01

    We describe an interactive visualization and modeling program for the creation of protein structures ''from scratch''. The input to our program is an amino acid sequence -decoded from a gene- and a sequence of predicted secondary structure types for each amino acid-provided by external structure prediction programs. Our program can be used in the set-up phase of a protein structure prediction process; the structures created with it serve as input for a subsequent global internal energy minimization, or another method of protein structure prediction. Our program supports basic visualization methods for protein structures, interactive manipulation based on inverse kinematics, and visualization guides to aid a user in creating ''good'' initial structures.

  19. C-reactive protein

    Science.gov (United States)

    ... body. It is one of a group of proteins called "acute phase reactants" that go up in response to inflammation. This article discusses the blood test done to measure the amount of CRP in your blood.

  20. Polymers for Protein Conjugation

    Directory of Open Access Journals (Sweden)

    Gianfranco Pasut

    2014-01-01

    Full Text Available Polyethylene glycol (PEG at the moment is considered the leading polymer for protein conjugation in view of its unique properties, as well as to its low toxicity in humans, qualities which have been confirmed by its extensive use in clinical practice. Other polymers that are safe, biodegradable and custom-designed have, nevertheless, also been investigated as potential candidates for protein conjugation. This review will focus on natural polymers and synthetic linear polymers that have been used for protein delivery and the results associated with their use. Genetic fusion approaches for the preparation of protein-polypeptide conjugates will be also reviewed and compared with the best known chemical conjugation ones.

  1. The Pentapeptide Repeat Proteins

    Energy Technology Data Exchange (ETDEWEB)

    Vetting,M.; Hegde, S.; Fajardo, J.; Fiser, A.; Roderick, S.; Takiff, H.; Blanchard, J.

    2006-01-01

    The Pentapeptide Repeat Protein (PRP) family has over 500 members in the prokaryotic and eukaryotic kingdoms. These proteins are composed of, or contain domains composed of, tandemly repeated amino acid sequences with a consensus sequence of [S, T,A, V][D, N][L, F]-[S, T,R][G]. The biochemical function of the vast majority of PRP family members is unknown. The three-dimensional structure of the first member of the PRP family was determined for the fluoroquinolone resistance protein (MfpA) from Mycobacterium tuberculosis. The structure revealed that the pentapeptide repeats encode the folding of a novel right-handed quadrilateral {beta}-helix. MfpA binds to DNA gyrase and inhibits its activity. The rod-shaped, dimeric protein exhibits remarkable size, shape and electrostatic similarity to DNA.

  2. Protein Colloidal Aggregation Project

    Science.gov (United States)

    Oliva-Buisson, Yvette J. (Compiler)

    2014-01-01

    To investigate the pathways and kinetics of protein aggregation to allow accurate predictive modeling of the process and evaluation of potential inhibitors to prevalent diseases including cataract formation, chronic traumatic encephalopathy, Alzheimer's Disease, Parkinson's Disease and others.

  3. Egg protein hydrolysates

    NARCIS (Netherlands)

    Amerongen, van A.; Beelen, M.J.C.; Wolbers, L.A.M.; Gilst, van W.H.; Buikema, J.H.; Nelissen, J.W.P.M.

    2009-01-01

    The present invention provides egg-protein hydrolysates with DPP-IV inhibitory activity which are particularly suited for the treatment of diabetes. Particularly advantageous is to use hydrolysate of lysozyme for the treatment of diabetes.

  4. Plant protein glycosylation

    Science.gov (United States)

    Strasser, Richard

    2016-01-01

    Protein glycosylation is an essential co- and post-translational modification of secretory and membrane proteins in all eukaryotes. The initial steps of N-glycosylation and N-glycan processing are highly conserved between plants, mammals and yeast. In contrast, late N-glycan maturation steps in the Golgi differ significantly in plants giving rise to complex N-glycans with β1,2-linked xylose, core α1,3-linked fucose and Lewis A-type structures. While the essential role of N-glycan modifications on distinct mammalian glycoproteins is already well documented, we have only begun to decipher the biological function of this ubiquitous protein modification in different plant species. In this review, I focus on the biosynthesis and function of different protein N-linked glycans in plants. Special emphasis is given on glycan-mediated quality control processes in the ER and on the biological role of characteristic complex N-glycan structures. PMID:26911286

  5. Markers of protein oxidation

    DEFF Research Database (Denmark)

    Headlam, Henrietta A; Davies, Michael Jonathan

    2004-01-01

    Exposure of proteins to radicals in the presence of O2 gives both side-chain oxidation and backbone fragmentation. These processes can be interrelated, with initial side-chain oxidation giving rise to backbone damage via transfer reactions. We have shown previously that alkoxyl radicals formed...... of this process depends on the extent of oxidation at C-3 compared with other sites. HO*, generated by gamma radiolysis, gave the highest total carbonyl yield, with protein-bound carbonyls predominating over released. In contrast, metal ion/H2O2 systems, gave more released than bound carbonyls, with this ratio...... modulated by EDTA. This is ascribed to metal ion-protein interactions affecting the sites of initial oxidation. Hypochlorous acid gave low concentrations of released carbonyls, but high yields of protein-bound material. The peroxyl radical generator 2,2'-azobis(2-amidinopropane) hydrochloride...

  6. Protein tyrosine nitration

    Science.gov (United States)

    Chaki, Mounira; Leterrier, Marina; Barroso, Juan B

    2009-01-01

    Nitric oxide metabolism in plant cells has a relative short history. Nitration is a chemical process which consists of introducing a nitro group (-NO2) into a chemical compound. in biological systems, this process has been found in different molecules such as proteins, lipids and nucleic acids that can affect its function. This mini-review offers an overview of this process with special emphasis on protein tyrosine nitration in plants and its involvement in the process of nitrosative stress. PMID:19826215

  7. Digestibility of sorghum proteins.

    OpenAIRE

    Axtell, J D; Kirleis, A. W.; Hassen, M M; D'Croz Mason, N; Mertz, E T; Munck, L.

    1981-01-01

    Published information indicates that rice, maize, and wheat proteins are much more digestible in children than sorghum proteins are (66-81% compared with 46%). However, this digestibility difference cannot be demonstrated with the weanling rat, which gave digestibility values of 80% for cooked and 85% for uncooked sorghum gruels. Therefore, a search was made for a laboratory system sensitive to the digestibility differences between sorghum and other cereals. We found that porcine pepsin in vi...

  8. Identifying Unknown Proteins

    OpenAIRE

    Barker, Winona C.; Dayhoff, Margaret O.

    1983-01-01

    In this paper we discuss ways to identify a protein, both when its amino acid sequence is known and, particularly, prior to the determination of the complete sequence. If a similar sequence is in the Protein Sequence Database, an unknown may be identified on the basis of partial or ambiguous sequence data, or on the basis of amino acid composition. Identification in the early stages of structural determination can save time and scarce resources by preventing duplicate effort or by suggesting ...

  9. Fish protein hydrolysates

    Energy Technology Data Exchange (ETDEWEB)

    Mackie, I.M.

    1982-01-01

    Proteolytic enzymes now available in commercial quantities can be used to liquefy the fish and fish waste presently considered suitable for conversion to fish meal. The products obtained are readily dispersed or dissolved in water and have a high nutritional value. They have been satisfactorily used as substitutes for milk proteins in milk replacers for young animals. Further research is necessary on means of controlling the degree of hydrolysis to give protein preparations with acceptable functional properties as human food supplements. (Refs. 21).

  10. The Malignant Protein Puzzle.

    Science.gov (United States)

    Walker, Lary C; Jucker, Mathias

    2016-01-01

    When most people hear the words malignant and brain, cancer immediately comes to mind. But our authors argue that proteins can be malignant too, and can spread harmfully through the brain in neurodegenerative diseases that include Alzheimer's, Parkinson's, CTE, and ALS. Studying how proteins such as PrP, amyloid beta, tau, and others aggregate and spread, and kill brain cells, represents a crucial new frontier in neuroscience. PMID:27408676

  11. Recombinant Collagenlike Proteins

    Science.gov (United States)

    Fertala, Andzej

    2007-01-01

    A group of collagenlike recombinant proteins containing high densities of biologically active sites has been invented. The method used to express these proteins is similar to a method of expressing recombinant procollagens and collagens described in U. S. Patent 5,593,859, "Synthesis of human procollagens and collagens in recombinant DNA systems." Customized collagenous proteins are needed for biomedical applications. In particular, fibrillar collagens are attractive for production of matrices needed for tissue engineering and drug delivery. Prior to this invention, there was no way of producing customized collagenous proteins for these and other applications. Heretofore, collagenous proteins have been produced by use of such biological systems as yeasts, bacteria, and transgenic animals and plants. These products are normal collagens that can also be extracted from such sources as tendons, bones, and hides. These products cannot be made to consist only of biologically active, specific amino acid sequences that may be needed for specific applications. Prior to this invention, it had been established that fibrillar collagens consist of domains that are responsible for such processes as interaction with cells, binding of growth factors, and interaction with a number of structural proteins present in the extracellular matrix. A normal collagen consists of a sequence of domains that can be represented by a corresponding sequence of labels, e.g., D1D2D3D4. A collagenlike protein of the present invention contains regions of collagen II that contain multiples of a single domain (e.g., D1D1D1D1 or D4D4D4D4) chosen for its specific biological activity. By virtue of the multiplicity of the chosen domain, the density of sites having that specific biological activity is greater than it is in a normal collagen. A collagenlike protein according to this invention can thus be made to have properties that are necessary for tissue engineering.

  12. The effect of protein-protein and protein-membrane interactions on membrane fouling in ultrafiltration

    NARCIS (Netherlands)

    Huisman, I.H.; Prádanos, P.; Hernández, A.

    2000-01-01

    It was studied how protein-protein and protein-membrane interactions influence the filtration performance during the ultrafiltration of protein solutions over polymeric membranes. This was done by measuring flux, streaming potential, and protein transmission during filtration of bovine serum albumin

  13. Bence-Jones protein - quantitative

    Science.gov (United States)

    Immunoglobulin light chains - urine; Urine Bence-Jones protein ... Bence-Jones proteins are a part of regular antibodies called light chains. These proteins are not normally in urine. Sometimes, when ...

  14. More protein in cereals?

    International Nuclear Information System (INIS)

    Ways in which the protein content of plant crops may be raised by the use of nuclear radiation are to be discussed at a symposium in Vienna in June next year, organized by the joint Food and Agriculture Organization/Agency Division of Atomic Energy in Food and Agriculture. Plant crops - especially cereal grains - are the basic food and protein source of most of the world's population, particularly in less-developed countries. But their natural protein content is low; increasing the quantity and nutritional quality of plant protein is potentially the most feasible way to combat widespread protein malnutrition. This improvement in seed stock can be achieved by plant breeding methods in which nuclear irradiation techniques are used to induce mutations in grain, and other isotopic techniques can be used to select only those mutants which have the desired properties. The scientists who attend the symposium will have an opportunity to review what mutation plant breeders have achieved, the application of nuclear techniques to screening for protein and amino-acid content and nutritional value, and isotopic methods which contribute to research in plant nutrition and physiology. (author)

  15. Hepatitis C virus proteins

    Institute of Scientific and Technical Information of China (English)

    Jean Dubuisson

    2007-01-01

    Hepatitis C virus (HCV) encodes a single polyprotein,which is processed by cellular and viral proteases to generate 10 polypeptides. The HCV genome also contains an overlapping +1 reading frame that may lead to the synthesis of an additional protein. Until recently,studies of HCV have been hampered by the lack of a productive cell culture system. Since the identification of HCV genome approximately 17 years ago, structural,biochemical and biological information on HCV proteins has mainly been obtained with proteins produced by heterologous expression systems. In addition, some functional studies have also been confirmed with replicon systems or with retroviral particles pseudotyped with HCV envelope glycoproteins. The data that have accumulated on HCV proteins begin to provide a framework for understanding the molecular mechanisms involved in the major steps of HCV life cycle. Moreover,the knowledge accumulated on HCV proteins is also leading to the development of antiviral drugs among which some are showing promising results in early-phase clinical trials. This review summarizes the current knowledge on the functions and biochemical features of HCV proteins.

  16. Cardiolipin Interactions with Proteins.

    Science.gov (United States)

    Planas-Iglesias, Joan; Dwarakanath, Himal; Mohammadyani, Dariush; Yanamala, Naveena; Kagan, Valerian E; Klein-Seetharaman, Judith

    2015-09-15

    Cardiolipins (CL) represent unique phospholipids of bacteria and eukaryotic mitochondria with four acyl chains and two phosphate groups that have been implicated in numerous functions from energy metabolism to apoptosis. Many proteins are known to interact with CL, and several cocrystal structures of protein-CL complexes exist. In this work, we describe the collection of the first systematic and, to the best of our knowledge, the comprehensive gold standard data set of all known CL-binding proteins. There are 62 proteins in this data set, 21 of which have nonredundant crystal structures with bound CL molecules available. Using binding patch analysis of amino acid frequencies, secondary structures and loop supersecondary structures considering phosphate and acyl chain binding regions together and separately, we gained a detailed understanding of the general structural and dynamic features involved in CL binding to proteins. Exhaustive docking of CL to all known structures of proteins experimentally shown to interact with CL demonstrated the validity of the docking approach, and provides a rich source of information for experimentalists who may wish to validate predictions.

  17. Protein hydrolysates in sports nutrition

    Directory of Open Access Journals (Sweden)

    Manninen Anssi H

    2009-09-01

    Full Text Available Abstract It has been suggested that protein hydrolysates providing mainly di- and tripeptides are superior to intact (whole proteins and free amino acids in terms of skeletal muscle protein anabolism. This review provides a critical examination of protein hydrolysate studies conducted in healthy humans with special reference to sports nutrition. The effects of protein hydrolysate ingestion on blood amino acid levels, muscle protein anabolism, body composition, exercise performance and muscle glycogen resynthesis are discussed.

  18. Protein Functionality in Food Systems

    Institute of Scientific and Technical Information of China (English)

    WANG Panpan

    2010-01-01

    The structure,shape,color,smell and taste of food were decided by protein functionality.The utilization of protein will improve by changing the protein functionality.Protein functionality is also advantage to maintain and utilize the nutrition of food.This paper summarized the nature,classification,factors and prospect of protein functionality.It ccn provide a theoretical basis for application of protein in food industry.

  19. Modeling Mercury in Proteins

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Jeremy C [ORNL; Parks, Jerry M [ORNL

    2016-01-01

    Mercury (Hg) is a naturally occurring element that is released into the biosphere both by natural processes and anthropogenic activities. Although its reduced, elemental form Hg(0) is relatively non-toxic, other forms such as Hg2+ and, in particular, its methylated form, methylmercury, are toxic, with deleterious effects on both ecosystems and humans. Microorganisms play important roles in the transformation of mercury in the environment. Inorganic Hg2+ can be methylated by certain bacteria and archaea to form methylmercury. Conversely, bacteria also demethylate methylmercury and reduce Hg2+ to relatively inert Hg(0). Transformations and toxicity occur as a result of mercury interacting with various proteins. Clearly, then, understanding the toxic effects of mercury and its cycling in the environment requires characterization of these interactions. Computational approaches are ideally suited to studies of mercury in proteins because they can provide a detailed picture and circumvent issues associated with toxicity. Here we describe computational methods for investigating and characterizing how mercury binds to proteins, how inter- and intra-protein transfer of mercury is orchestrated in biological systems, and how chemical reactions in proteins transform the metal. We describe quantum chemical analyses of aqueous Hg(II), which reveal critical factors that determine ligand binding propensities. We then provide a perspective on how we used chemical reasoning to discover how microorganisms methylate mercury. We also highlight our combined computational and experimental studies of the proteins and enzymes of the mer operon, a suite of genes that confers mercury resistance in many bacteria. Lastly, we place work on mercury in proteins in the context of what is needed for a comprehensive multi-scale model of environmental mercury cycling.

  20. PROTEIN SYNTHESIS GAME

    Directory of Open Access Journals (Sweden)

    J.C.Q. Carvalho

    2004-05-01

    Full Text Available The theoretical explanation of biological concepts, associated with the use of teaching games andmodels, intensify the comprehension and increase students interest, stimulating them to participateactively on the teaching-learning process. The sta of dissemination from Centro de BiotecnologiaMolecular Estrutural (CBME, in partnership with the Centro de Divulgac~ao Cientca e Cultural(CDCC, presents, in this work, a new educational resource denoted: Protein Synthesis Game. Theapproach of the game involves the cytological aspects of protein synthesis, directed to high schoolstudents. Students are presented to day-by-day facts related to the function of a given protein in thehuman body. Such task leads players to the goal of solving out a problem through synthesizing aspecied protein. The game comprises: (1 a board illustrated with the transversal section of animalcell, with its main structures and organelles and sequences of hypothetical genes; (2 cards with thedescription of steps and other structures required for protein synthesis in eukaryotic cells; (3 piecesrepresenting nucleotides, polynucleotides, ribosome, amino acids, and polypeptide chains. In order toplay the game, students take cards that sequentially permit them to acquire the necessary pieces forproduction of the protein described in each objective. Players must move the pieces on the board andsimulate the steps of protein synthesis. The dynamic of the game allows students to easily comprehendprocesses of transcription and translation. This game was presented to dierent groups of high schoolteachers and students. Their judgments have been heard and indicated points to be improved, whichhelped us with the game development. Furthermore, the opinions colleted were always favorable forthe application of this game as a teaching resource in classrooms.

  1. Inferring protein function by domain context similarities in protein-protein interaction networks

    OpenAIRE

    Sun Zhirong; Liu Ke; Chen Hu; Zhang Song

    2009-01-01

    Abstract Background Genome sequencing projects generate massive amounts of sequence data but there are still many proteins whose functions remain unknown. The availability of large scale protein-protein interaction data sets makes it possible to develop new function prediction methods based on protein-protein interaction (PPI) networks. Although several existing methods combine multiple information resources, there is no study that integrates protein domain information and PPI networks to pre...

  2. ADSORPTION OF PROTEIN ON NANOPARTICLES

    Institute of Scientific and Technical Information of China (English)

    WU Qi

    1994-01-01

    The adsorption of protein on nanoparticles was studied by using dynamic light scattering to measure the hydrodynamic size of both pure protein and nanoparticles adsorbed with different amounts of protein. The thickness of the adsorbed protein layer increases as protein concentration, but decreases as the initial size of nanoparticles. After properly scaling the thickness with the initial diameter, we are able to fit all experimental data with a single master curve. Our experimental results suggest that the adsorbed proteins form a monolayeron the nanoparticle surface and the adsorbed protein molecules are attached to the particle surface at many points through a possible hydrogen-bonding. Our results also indicate that as protein concentration increases, the overall shape of the adsorbed protein molecule continuously changes from a flat layer on the particle surface to a stretched coil extended into water. During the change, the hydrodynamic volume of the adsorbed protein increases linearly with protein concentration.

  3. Measuring protein breakdown rate in individual proteins in vivo

    DEFF Research Database (Denmark)

    Holm, Lars; Kjaer, Michael

    2010-01-01

    To outline different approaches of how protein breakdown can be quantified and to present a new approach to determine the fractional breakdown rate of individual slow turnover proteins in vivo.......To outline different approaches of how protein breakdown can be quantified and to present a new approach to determine the fractional breakdown rate of individual slow turnover proteins in vivo....

  4. Benchtop Detection of Proteins

    Science.gov (United States)

    Scardelletti, Maximilian C.; Varaljay, Vanessa

    2007-01-01

    A process, and a benchtop-scale apparatus for implementing the process, have been developed to detect proteins associated with specific microbes in water. The process and apparatus may also be useful for detection of proteins in other, more complex liquids. There may be numerous potential applications, including monitoring lakes and streams for contamination, testing of blood and other bodily fluids in medical laboratories, and testing for microbial contamination of liquids in restaurants and industrial food-processing facilities. A sample can be prepared and analyzed by use of this process and apparatus within minutes, whereas an equivalent analysis performed by use of other processes and equipment can often take hours to days. The process begins with the conjugation of near-infrared-fluorescent dyes to antibodies that are specific to a particular protein. Initially, the research has focused on using near-infrared dyes to detect antigens or associated proteins in solution, which has proven successful vs. microbial cells, and streamlining the technique in use for surface protein detection on microbes would theoretically render similar results. However, it is noted that additional work is needed to transition protein-based techniques to microbial cell detection. Consequently, multiple such dye/antibody pairs could be prepared to enable detection of multiple selected microbial species, using a different dye for each species. When excited by near-infrared light of a suitable wavelength, each dye fluoresces at a unique longer wavelength that differs from those of the other dyes, enabling discrimination among the various species. In initial tests, the dye/antibody pairs are mixed into a solution suspected of containing the selected proteins, causing the binding of the dye/antibody pairs to such suspect proteins that may be present. The solution is then run through a microcentrifuge that includes a membrane that acts as a filter in that it retains the dye/antibody/protein

  5. Measuring protein breakdown in individual proteins in vivo

    DEFF Research Database (Denmark)

    Holm, Lars; Kjær, Michael

    2010-01-01

    is that the proteins of interest are the site of measurement. Hence, the application initially demands the proteins to be labeled with stable isotopically labeled amino acids. Subsequently, the loss of label from the proteins will be dependent on the protein breakdown rate when no labeled amino acids......PURPOSE OF REVIEW: To outline different approaches of how protein breakdown can be quantified and to present a new approach to determine the fractional breakdown rate of individual slow turnover proteins in vivo. RECENT FINDINGS: None of the available methods for determining protein breakdown can...

  6. Immunocytochemical heterogeneity of somatostatin-expressing GABAergic interneurons in layers II and III of the mouse cingulate cortex: A combined immunofluorescence/design-based stereologic study.

    Science.gov (United States)

    Riedemann, Therese; Schmitz, Christoph; Sutor, Bernd

    2016-08-01

    Many neurological diseases including major depression and schizophrenia manifest as dysfunction of the GABAergic system within the cingulate cortex. However, relatively little is known about the properties of GABAergic interneurons in the cingulate cortex. Therefore, we investigated the neurochemical properties of GABAergic interneurons in the cingulate cortex of FVB-Tg(GadGFP)45704Swn/J mice expressing green fluorescent protein (GFP) in a subset of GABAergic interneurons (GFP-expressing inhibitory interneurons [GINs]) by means of immunocytochemical and design-based stereologic techniques. We found that GINs represent around 12% of all GABAergic interneurons in the cingulate cortex. In contrast to other neocortical areas, GINs were only found in cortical layers II and III. More than 98% of GINs coexpressed the neuropeptide somatostatin (SOM), but only 50% of all SOM + neurons were GINs. By analyzing the expression of calretinin (CR), calbindin (CB), parvalbumin, and various neuropeptides, we identified several distinct GIN subgroups. In particular, we observed coexpression of SOM with CR and CB. In addition, we found neuropeptide Y expression almost exclusively in those GINs that coexpressed SOM and CR. Thus, with respect to the expression of calcium-binding proteins and neuropeptides, GINs are surprisingly heterogeneous in the mouse cingulate cortex, and the minority of GINs express only one marker protein or peptide. Furthermore, our observation of overlap between the SOM + and CR + interneuron population was in contrast to earlier findings of non-overlapping SOM + and CR + interneuron populations in the human cortex. This might indicate that findings in mouse models of neuropsychiatric diseases may not be directly transferred to human patients. J. Comp. Neurol. 524:2281-2299, 2016. © 2015 Wiley Periodicals, Inc. PMID:26669716

  7. Ontology integration to identify protein complex in protein interaction networks

    OpenAIRE

    Yang Zhihao; Lin Hongfei; Xu Bo

    2011-01-01

    Abstract Background Protein complexes can be identified from the protein interaction networks derived from experimental data sets. However, these analyses are challenging because of the presence of unreliable interactions and the complex connectivity of the network. The integration of protein-protein interactions with the data from other sources can be leveraged for improving the effectiveness of protein complexes detection algorithms. Methods We have developed novel semantic similarity metho...

  8. Identifying Protein-Protein Interaction Sites Using Covering Algorithm

    OpenAIRE

    Jie Song; Jiaxing Cheng; Xiuquan Du

    2009-01-01

    Identification of protein-protein interface residues is crucial for structural biology. This paper proposes a covering algorithm for predicting protein-protein interface residues with features including protein sequence profile and residue accessible area. This method adequately utilizes the characters of a covering algorithm which have simple, lower complexity and high accuracy for high dimension data. The covering algorithm can achieve a comparable performance (69.62%, Complete dataset; 60....

  9. Protein-Protein Interaction Detection: Methods and Analysis

    OpenAIRE

    V. Srinivasa Rao; Srinivas, K.; Sujini, G. N.; G. N. Sunand Kumar

    2014-01-01

    Protein-protein interaction plays key role in predicting the protein function of target protein and drug ability of molecules. The majority of genes and proteins realize resulting phenotype functions as a set of interactions. The in vitro and in vivo methods like affinity purification, Y2H (yeast 2 hybrid), TAP (tandem affinity purification), and so forth have their own limitations like cost, time, and so forth, and the resultant data sets are noisy and have more false positives to annotate t...

  10. Polarizable protein packing

    KAUST Repository

    Ng, Albert H.

    2011-01-24

    To incorporate protein polarization effects within a protein combinatorial optimization framework, we decompose the polarizable force field AMOEBA into low order terms. Including terms up to the third-order provides a fair approximation to the full energy while maintaining tractability. We represent the polarizable packing problem for protein G as a hypergraph and solve for optimal rotamers with the FASTER combinatorial optimization algorithm. These approximate energy models can be improved to high accuracy [root mean square deviation (rmsd) < 1 kJ mol -1] via ridge regression. The resulting trained approximations are used to efficiently identify new, low-energy solutions. The approach is general and should allow combinatorial optimization of other many-body problems. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011 Copyright © 2011 Wiley Periodicals, Inc.

  11. Trisulfides in Proteins

    DEFF Research Database (Denmark)

    Nielsen, Rasmus W.; Tachibana, Christine; Hansen, Niels Erik;

    2011-01-01

    Trisulfides and other oligosulfides are widely distributed in the biological world. In plants, e.g., garlic, trisulfides are associated with potentially beneficial properties. However, an extra neutral sulfur atom covalently bound between the two sulfur atoms of a pair of cysteines is not a commo...... post-translational modification, and the number of proteins in which a trisulfide has been unambiguously identified is small. Nevertheless, we believe that its prevalence may be underestimated, particularly with the increasing evidence for significant pools of sulfides in living tissues...... and their possible roles in cellular metabolism. This review focuses on examples of proteins that are known to contain a trisulfide bridge, and gives an overview of the chemistry of trisulfide formation, and the methods by which it is detected in proteins....

  12. Accessory Proteins at ERES

    DEFF Research Database (Denmark)

    Klinkenberg, Rafael David

    proteins. Together these components co‐operate in cargo‐selection as well as forming, loading and releasing budding vesicles from specific regions on the membrane surface of the ER. Coat components furthermore convey vesicle targeting towards the Golgi. However, not much is known about the mechanisms...... that regulate the COPII assembly at the vesicle bud site. This thesis provides the first regulatory mechanism of COPII assembly in relation to ER‐membrane lipid‐signal recognition by the accessory protein p125A (Sec23IP). The aim of the project was to characterize p125A function by dissecting two main domains...... in the protein; a putative lipid‐associating domain termed the DDHD domain that is defined by the four amino acid motif that gives the domain its name; and a ubiquitously found domain termed Sterile α‐motif (SAM), which is mostly associated with oligomerization and polymerization. We first show, that the DDHD...

  13. Electron transfer in proteins.

    Science.gov (United States)

    Gray, H B; Winkler, J R

    1996-01-01

    Electron-transfer (ET) reactions are key steps in a diverse array of biological transformations ranging from photosynthesis to aerobic respiration. A powerful theoretical formalism has been developed that describes ET rates in terms of two parameters: the nuclear reorganization energy (lambda) and the electronic-coupling strength (HAB). Studies of ET reactions in ruthenium-modified proteins have probed lambda and HAB in several metalloproteins (cytochrome c, myoglobin, azurin). This work has shown that protein reorganization energies are sensitive to the medium surrounding the redox sites and that an aqueous environment, in particular, leads to large reorganization energies. Analyses of electronic-coupling strengths suggest that the efficiency of long-range ET depends on the protein secondary structure: beta sheets appear to mediate coupling more efficiently than alpha-helical structures, and hydrogen bonds play a critical role in both. PMID:8811189

  14. Discovery of binding proteins for a protein target using protein-protein docking-based virtual screening.

    Science.gov (United States)

    Zhang, Changsheng; Tang, Bo; Wang, Qian; Lai, Luhua

    2014-10-01

    Target structure-based virtual screening, which employs protein-small molecule docking to identify potential ligands, has been widely used in small-molecule drug discovery. In the present study, we used a protein-protein docking program to identify proteins that bind to a specific target protein. In the testing phase, an all-to-all protein-protein docking run on a large dataset was performed. The three-dimensional rigid docking program SDOCK was used to examine protein-protein docking on all protein pairs in the dataset. Both the binding affinity and features of the binding energy landscape were considered in the scoring function in order to distinguish positive binding pairs from negative binding pairs. Thus, the lowest docking score, the average Z-score, and convergency of the low-score solutions were incorporated in the analysis. The hybrid scoring function was optimized in the all-to-all docking test. The docking method and the hybrid scoring function were then used to screen for proteins that bind to tumor necrosis factor-α (TNFα), which is a well-known therapeutic target for rheumatoid arthritis and other autoimmune diseases. A protein library containing 677 proteins was used for the screen. Proteins with scores among the top 20% were further examined. Sixteen proteins from the top-ranking 67 proteins were selected for experimental study. Two of these proteins showed significant binding to TNFα in an in vitro binding study. The results of the present study demonstrate the power and potential application of protein-protein docking for the discovery of novel binding proteins for specific protein targets.

  15. Modeling Mercury in Proteins.

    Science.gov (United States)

    Parks, J M; Smith, J C

    2016-01-01

    Mercury (Hg) is a naturally occurring element that is released into the biosphere both by natural processes and anthropogenic activities. Although its reduced, elemental form Hg(0) is relatively nontoxic, other forms such as Hg(2+) and, in particular, its methylated form, methylmercury, are toxic, with deleterious effects on both ecosystems and humans. Microorganisms play important roles in the transformation of mercury in the environment. Inorganic Hg(2+) can be methylated by certain bacteria and archaea to form methylmercury. Conversely, bacteria also demethylate methylmercury and reduce Hg(2+) to relatively inert Hg(0). Transformations and toxicity occur as a result of mercury interacting with various proteins. Clearly, then, understanding the toxic effects of mercury and its cycling in the environment requires characterization of these interactions. Computational approaches are ideally suited to studies of mercury in proteins because they can provide a detailed molecular picture and circumvent issues associated with toxicity. Here, we describe computational methods for investigating and characterizing how mercury binds to proteins, how inter- and intraprotein transfer of mercury is orchestrated in biological systems, and how chemical reactions in proteins transform the metal. We describe quantum chemical analyses of aqueous Hg(II), which reveal critical factors that determine ligand-binding propensities. We then provide a perspective on how we used chemical reasoning to discover how microorganisms methylate mercury. We also highlight our combined computational and experimental studies of the proteins and enzymes of the mer operon, a suite of genes that confer mercury resistance in many bacteria. Lastly, we place work on mercury in proteins in the context of what is needed for a comprehensive multiscale model of environmental mercury cycling. PMID:27497164

  16. Protein: FEA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEA3 AREB pathway: Signaling proteins AZF1 OZAKGYO, ZF1 At5g67450, Cys2/His2-type zinc finger protein... 1, Zinc finger protein OZAKGYO, Zinc-finger protein 1 3702 Arabidopsis thaliana 836881 Q9SSW1 21852415 ...

  17. Protein: FEA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEA3 AREB pathway: Signaling proteins SRK2E OST1, SNRK2.6 Serine/threonine-protein kinase SRK2E Protein... OPEN STOMATA 1, SNF1-related kinase 2.6, Serine/threonine-protein kinase OST1 3702 Arabidopsis thaliana 829541 Q940H6 3UC4, 3ZUT, 3ZUU, 3UDB 19805022 ...

  18. Protein: FBB5 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBB5 RNA silencing TNRC6A CAGH26, KIAA1460, TNRC6 TNRC6A Trinucleotide repeat-containing gene 6A protein... CAG repeat protein 26, EMSY interactor protein, GW182 autoantigen, Glycine-tryptophan protein of 182 kDa 9606 Homo sapiens Q8NDV7 27327 27327 19398495 ...

  19. Ubiquitin domain proteins in disease

    DEFF Research Database (Denmark)

    Klausen, Louise Kjær; Schulze, Andrea; Seeger, Michael;

    2007-01-01

    The human genome encodes several ubiquitin-like (UBL) domain proteins (UDPs). Members of this protein family are involved in a variety of cellular functions and many are connected to the ubiquitin proteasome system, an essential pathway for protein degradation in eukaryotic cells. Despite...... and cancer. Publication history: Republished from Current BioData's Targeted Proteins database (TPdb; http://www.targetedproteinsdb.com)....

  20. Mining protein structure data

    OpenAIRE

    Santos, José Carlos Almeida

    2006-01-01

    The principal topic of this work is the application of data mining techniques, in particular of machine learning, to the discovery of knowledge in a protein database. In the first chapter a general background is presented. Namely, in section 1.1 we overview the methodology of a Data Mining project and its main algorithms. In section 1.2 an introduction to the proteins and its supporting file formats is outlined. This chapter is concluded with section 1.3 which defines that main problem we...

  1. Lipid-transfer proteins.

    Science.gov (United States)

    Ng, Tzi Bun; Cheung, Randy Chi Fai; Wong, Jack Ho; Ye, Xiujuan

    2012-01-01

    Lipid-transfer proteins (LTPs) are basic proteins found in abundance in higher plants. LTPs play lots of roles in plants such as participation in cutin formation, embryogenesis, defense reactions against phytopathogens, symbiosis, and the adaptation of plants to various environmental conditions. In addition, LTPs from field mustard and Chinese daffodil exhibit antiproliferative activity against human cancer cells. LTPs from chili pepper and coffee manifest inhibitory activity against fungi pathogenic to humans such as Candida species. The intent of this article is to review LTPs in the plant kingdom. PMID:23193591

  2. Water-transporting proteins

    DEFF Research Database (Denmark)

    Zeuthen, Thomas

    2010-01-01

    Transport through lipids and aquaporins is osmotic and entirely driven by the difference in osmotic pressure. Water transport in cotransporters and uniporters is different: Water can be cotransported, energized by coupling to the substrate flux by a mechanism closely associated with protein...... is not clear. It is associated with the substrate movements in aqueous pathways within the protein; a conventional unstirred layer mechanism can be ruled out, due to high rates of diffusion in the cytoplasm. The physiological roles of the various modes of water transport are reviewed in relation to epithelial...

  3. Modelling of proteins in membranes

    DEFF Research Database (Denmark)

    Sperotto, Maria Maddalena; May, S.; Baumgaertner, A.

    2006-01-01

    This review describes some recent theories and simulations of mesoscopic and microscopic models of lipid membranes with embedded or attached proteins. We summarize results supporting our understanding of phenomena for which the activities of proteins in membranes are expected to be significantly...... affected by the lipid environment. Theoretical predictions are pointed out, and compared to experimental findings, if available. Among others, the following phenomena are discussed: interactions of interfacially adsorbed peptides, pore-forming amphipathic peptides, adsorption of charged proteins onto...... oppositely charged lipid membranes, lipid-induced tilting of proteins embedded in lipid bilayers, protein-induced bilayer deformations, protein insertion and assembly, and lipid-controlled functioning of membrane proteins....

  4. Conformation Distributions in Adsorbed Proteins.

    Science.gov (United States)

    Meuse, Curtis W.; Hubbard, Joseph B.; Vrettos, John S.; Smith, Jackson R.; Cicerone, Marcus T.

    2007-03-01

    While the structural basis of protein function is well understood in the biopharmaceutical and biotechnology industries, few methods for the characterization and comparison of protein conformation distributions are available. New methods capable of measuring the stability of protein conformations and the integrity of protein-protein, protein-ligand and protein-surface interactions both in solution and on surfaces are needed to help the development of protein-based products. We are developing infrared spectroscopy methods for the characterization and comparison of molecular conformation distributions in monolayers and in solutions. We have extracted an order parameter describing the orientational and conformational variations of protein functional groups around the average molecular values from a single polarized spectrum. We will discuss the development of these methods and compare them to amide hydrogen/deuterium exchange methods for albumin in solution and on different polymer surfaces to show that our order parameter is related to protein stability.

  5. Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosis.

    OpenAIRE

    Benton, B K; Tinkelenberg, A; Gonzalez, I.; Cross, F R

    1997-01-01

    Yeasts have three functionally redundant G1 cyclins required for cell cycle progression through G1. Mutations in GIN4 and CLA4 were isolated in a screen for mutants that are inviable with deletions in the G1 cyclins CLN1 and CLN2. cln1 cln2 cla4 and cln1 cln2 gin4 cells arrest with a cytokinesis defect; this defect was efficiently rescued by CLN1 or CLN2 expression. GIN4 encodes a protein with strong homology to the Snflp serine/threonine kinase. Cla4p is homologous to mammalian p21-activated...

  6. Integral UBL domain proteins: a family of proteasome interacting proteins

    DEFF Research Database (Denmark)

    Hartmann-Petersen, Rasmus; Gordon, Colin

    2004-01-01

    The family of ubiquitin-like (UBL) domain proteins (UDPs) comprises a conserved group of proteins involved in a multitude of different cellular activities. However, recent studies on UBL-domain proteins indicate that these proteins appear to share a common property in their ability to interact with......-domain proteins catalyse the formation of ubiquitin-protein conjugates, whereas others appear to target ubiquitinated proteins for degradation and interact with chaperones. Hence, by binding to the 26S proteasome the UBL-domain proteins seem to tailor and direct the basic proteolytic functions of the particle to...... 26S proteasomes. The 26S proteasome is a multisubunit protease which is responsible for the majority of intracellular proteolysis in eukaryotic cells. Before degradation commences most proteins are first marked for destruction by being coupled to a chain of ubiquitin molecules. Some UBL...

  7. Interaction between plate make and protein in protein crystallisation screening.

    Directory of Open Access Journals (Sweden)

    Gordon J King

    Full Text Available BACKGROUND: Protein crystallisation screening involves the parallel testing of large numbers of candidate conditions with the aim of identifying conditions suitable as a starting point for the production of diffraction quality crystals. Generally, condition screening is performed in 96-well plates. While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystallisation plate. METHODOLOGY/PRINCIPAL FINDINGS: We performed a statistically rigorous examination of protein crystallisation, and evaluated interactions between crystallisation success and plate row/column, different plates of same make, different plate makes and different proteins. From our analysis of protein crystallisation, we found a significant interaction between plate make and the specific protein being crystallised. CONCLUSIONS/SIGNIFICANCE: Protein crystal structure determination is the principal method for determining protein structure but is limited by the need to produce crystals of the protein under study. Many important proteins are difficult to crystallize, so that identification of factors that assist crystallisation could open up the structure determination of these more challenging targets. Our findings suggest that protein crystallisation success may be improved by matching a protein with its optimal plate make.

  8. HIV protein sequence hotspots for crosstalk with host hub proteins.

    Directory of Open Access Journals (Sweden)

    Mahdi Sarmady

    Full Text Available HIV proteins target host hub proteins for transient binding interactions. The presence of viral proteins in the infected cell results in out-competition of host proteins in their interaction with hub proteins, drastically affecting cell physiology. Functional genomics and interactome datasets can be used to quantify the sequence hotspots on the HIV proteome mediating interactions with host hub proteins. In this study, we used the HIV and human interactome databases to identify HIV targeted host hub proteins and their host binding partners (H2. We developed a high throughput computational procedure utilizing motif discovery algorithms on sets of protein sequences, including sequences of HIV and H2 proteins. We identified as HIV sequence hotspots those linear motifs that are highly conserved on HIV sequences and at the same time have a statistically enriched presence on the sequences of H2 proteins. The HIV protein motifs discovered in this study are expressed by subsets of H2 host proteins potentially outcompeted by HIV proteins. A large subset of these motifs is involved in cleavage, nuclear localization, phosphorylation, and transcription factor binding events. Many such motifs are clustered on an HIV sequence in the form of hotspots. The sequential positions of these hotspots are consistent with the curated literature on phenotype altering residue mutations, as well as with existing binding site data. The hotspot map produced in this study is the first global portrayal of HIV motifs involved in altering the host protein network at highly connected hub nodes.

  9. Protein Molecular Structures, Protein SubFractions, and Protein Availability Affected by Heat Processing: A Review

    Directory of Open Access Journals (Sweden)

    Peiqiang Yu

    2007-01-01

    Full Text Available The utilization and availability of protein depended on the types of protein and their specific susceptibility to enzymatic hydrolysis (inhibitory activities in the gastrointestine and was highly associated with protein molecular structures. Studying internal protein structure and protein subfraction profiles leaded to an understanding of the components that make up a whole protein. An understanding of the molecular structure of the whole protein was often vital to understanding its digestive behavior and nutritive value in animals. In this review, recently obtained information on protein molecular structural effects of heat processing was reviewed, in relation to protein characteristics affecting digestive behavior and nutrient utilization and availability. The emphasis of this review was on (1 using the newly advanced synchrotron technology (S-FTIR as a novel approach to reveal protein molecular chemistry affected by heat processing within intact plant tissues; (2 revealing the effects of heat processing on the profile changes of protein subfractions associated with digestive behaviors and kinetics manipulated by heat processing; (3 prediction of the changes of protein availability and supply after heat processing, using the advanced DVE/OEB and NRC-2001 models, and (4 obtaining information on optimal processing conditions of protein as intestinal protein source to achieve target values for potential high net absorbable protein in the small intestine. The information described in this article may give better insight in the mechanisms involved and the intrinsic protein molecular structural changes occurring upon processing.

  10. Characterization of Protein Complexes and Subcomplexes in Protein-Protein Interaction Databases

    OpenAIRE

    Nazar Zaki; Elfadil A. Mohamed; Antonio Mora

    2015-01-01

    The identification and characterization of protein complexes implicated in protein-protein interaction data are crucial to the understanding of the molecular events under normal and abnormal physiological conditions. This paper provides a novel characterization of subcomplexes in protein interaction databases, stressing definition and representation issues, quantification, biological validation, network metrics, motifs, modularity, and gene ontology (GO) terms. The paper introduces the concep...

  11. Protein Thin Film Machines

    OpenAIRE

    Federici, Stefania; Oliviero, Giulio; Hamad-Schifferli, Kimberly; Bergese, Paolo

    2010-01-01

    We report the first example of microcantilever beams that are reversibly driven by protein thin film machines fuelled by cycling the salt concentration of the surrounding solution. We also show that upon the same salinity stimulus the drive can be completely reversed in its direction by introducing a surface coating ligand. Experimental results are throughout discussed within a general yet simple thermodynamic model.

  12. Protein: CAD [Trypanosomes Database

    Lifescience Database Archive (English)

    Full Text Available CAD carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotaseCA...D trifunctional proteincarbamoylphosphate synthetase 2/aspartate transcarbamylase/dihydroorotasemultifunctional protein CA...D H.sapiens 47458828 18105007 790 P27708 CAD_(gene) 2.1.3.2|3.5.2.3|6.3.5.5 114010 2p22-p21 hsa00250|hsa00240 ...

  13. Cellulose binding domain proteins

    Energy Technology Data Exchange (ETDEWEB)

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc (Davis, CA); Doi, Roy (Davis, CA)

    1998-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  14. Electron transfer in proteins

    DEFF Research Database (Denmark)

    Farver, O; Pecht, I

    1991-01-01

    Electron migration between and within proteins is one of the most prevalent forms of biological energy conversion processes. Electron transfer reactions take place between active centers such as transition metal ions or organic cofactors over considerable distances at fast rates and with remarkab...

  15. Protein Requirements during Aging

    Directory of Open Access Journals (Sweden)

    Glenda Courtney-Martin

    2016-08-01

    Full Text Available Protein recommendations for elderly, both men and women, are based on nitrogen balance studies. They are set at 0.66 and 0.8 g/kg/day as the estimated average requirement (EAR and recommended dietary allowance (RDA, respectively, similar to young adults. This recommendation is based on single linear regression of available nitrogen balance data obtained at test protein intakes close to or below zero balance. Using the indicator amino acid oxidation (IAAO method, we estimated the protein requirement in young adults and in both elderly men and women to be 0.9 and 1.2 g/kg/day as the EAR and RDA, respectively. This suggests that there is no difference in requirement on a gender basis or on a per kg body weight basis between younger and older adults. The requirement estimates however are ~40% higher than the current protein recommendations on a body weight basis. They are also 40% higher than our estimates in young men when calculated on the basis of fat free mass. Thus, current recommendations may need to be re-assessed. Potential rationale for this difference includes a decreased sensitivity to dietary amino acids and increased insulin resistance in the elderly compared with younger individuals.

  16. Tuber storage proteins.

    Science.gov (United States)

    Shewry, Peter R

    2003-06-01

    A wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. These are potato (Solanum tuberosum), cassava (Manihot esculenta), sweet potato (Ipomoea batatus), yams (Dioscorea spp.) and taro (Colocasia, Cyrtosperma and Xanthosoma spp.). All of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. Thus, patatin from potato exhibits activity as an acylhydrolase and esterase, sporamin from sweet potato is an inhibitor of trypsin, and dioscorin from yam is a carbonic anhydrase. Both sporamin and dioscorin also exhibit antioxidant and radical scavenging activity. Taro differs from the other three crops in that it contains two major types of storage protein: a trypsin inhibitor related to sporamin and a mannose-binding lectin. These characteristics indicate that tuber storage proteins have evolved independently in different species, which contrasts with the highly conserved families of storage proteins present in seeds. Furthermore, all exhibit biological activities which could contribute to resistance to pests, pathogens or abiotic stresses, indicating that they may have dual roles in the tubers.

  17. Protein Requirements during Aging.

    Science.gov (United States)

    Courtney-Martin, Glenda; Ball, Ronald O; Pencharz, Paul B; Elango, Rajavel

    2016-01-01

    Protein recommendations for elderly, both men and women, are based on nitrogen balance studies. They are set at 0.66 and 0.8 g/kg/day as the estimated average requirement (EAR) and recommended dietary allowance (RDA), respectively, similar to young adults. This recommendation is based on single linear regression of available nitrogen balance data obtained at test protein intakes close to or below zero balance. Using the indicator amino acid oxidation (IAAO) method, we estimated the protein requirement in young adults and in both elderly men and women to be 0.9 and 1.2 g/kg/day as the EAR and RDA, respectively. This suggests that there is no difference in requirement on a gender basis or on a per kg body weight basis between younger and older adults. The requirement estimates however are ~40% higher than the current protein recommendations on a body weight basis. They are also 40% higher than our estimates in young men when calculated on the basis of fat free mass. Thus, current recommendations may need to be re-assessed. Potential rationale for this difference includes a decreased sensitivity to dietary amino acids and increased insulin resistance in the elderly compared with younger individuals. PMID:27529275

  18. Protein–protein interactions

    NARCIS (Netherlands)

    Janin, J.; Bonvin, A.M.J.J.

    2013-01-01

    We are proud to present the first edition of the Protein–protein interactions Section of Current Opinion in Structural Biology. The Section is new, but the topic has been present in the journal from the very start. Volume 1, Issue 1, dated February 1991, had a review by Janin entitled Protein–protei

  19. Thermodynamics of meat proteins

    NARCIS (Netherlands)

    Sman, van der R.G.M.

    2012-01-01

    We describe the water activity of meat, being a mixture of proteins, salts and water, by the Free-Volume-Flory–Huggins (FVFH) theory augmented with the equation. Earlier, the FVFH theory is successfully applied to describe the thermodynamics to glucose homopolymers like starch, dextrans and maltodex

  20. Transient protein-protein interactions visualized by solution NMR.

    Science.gov (United States)

    Liu, Zhu; Gong, Zhou; Dong, Xu; Tang, Chun

    2016-01-01

    Proteins interact with each other to establish their identities in cell. The affinities for the interactions span more than ten orders of magnitude, and KD values in μM-mM regimen are considered transient and are important in cell signaling. Solution NMR including diamagnetic and paramagnetic techniques has enabled atomic-resolution depictions of transient protein-protein interactions. Diamagnetic NMR allows characterization of protein complexes with KD values up to several mM, whereas ultraweak and fleeting complexes can be modeled with the use of paramagnetic NMR especially paramagnetic relaxation enhancement (PRE). When tackling ever-larger protein complexes, PRE can be particularly useful in providing long-range intermolecular distance restraints. As NMR measurements are averaged over the ensemble of complex structures, structural information for dynamic protein-protein interactions besides the stereospecific one can often be extracted. Herein the protein interaction dynamics are exemplified by encounter complexes, alternative binding modes, and coupled binding/folding of intrinsically disordered proteins. Further integration of NMR with other biophysical techniques should allow better visualization of transient protein-protein interactions. In particular, single-molecule data may facilitate the interpretation of ensemble-averaged NMR data. Though same structures of proteins and protein complexes were found in cell as in diluted solution, we anticipate that the dynamics of transient protein protein-protein interactions be different, which awaits awaits exploration by NMR. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions. PMID:25896389

  1. Bayesian Estimator of Protein-Protein Association Probabilities

    Energy Technology Data Exchange (ETDEWEB)

    2008-05-28

    The Bayesian Estimator of Protein-Protein Association Probabilities (BEPro3) is a software tool for estimating probabilities of protein-protein association between bait and prey protein pairs using data from multiple-bait, multiple-replicate, protein LC-MS/MS affinity isolation experiments. BEPro3 is public domain software, has been tested on Windows XP and version 10.4 or newer of the Mac OS 10.4, and is freely available. A user guide, example dataset with analysis and additional documentation are included with the BEPro3 download.

  2. High quality protein microarray using in situ protein purification

    Directory of Open Access Journals (Sweden)

    Fleischmann Robert D

    2009-08-01

    Full Text Available Abstract Background In the postgenomic era, high throughput protein expression and protein microarray technologies have progressed markedly permitting screening of therapeutic reagents and discovery of novel protein functions. Hexa-histidine is one of the most commonly used fusion tags for protein expression due to its small size and convenient purification via immobilized metal ion affinity chromatography (IMAC. This purification process has been adapted to the protein microarray format, but the quality of in situ His-tagged protein purification on slides has not been systematically evaluated. We established methods to determine the level of purification of such proteins on metal chelate-modified slide surfaces. Optimized in situ purification of His-tagged recombinant proteins has the potential to become the new gold standard for cost-effective generation of high-quality and high-density protein microarrays. Results Two slide surfaces were examined, chelated Cu2+ slides suspended on a polyethylene glycol (PEG coating and chelated Ni2+ slides immobilized on a support without PEG coating. Using PEG-coated chelated Cu2+ slides, consistently higher purities of recombinant proteins were measured. An optimized wash buffer (PBST composed of 10 mM phosphate buffer, 2.7 mM KCl, 140 mM NaCl and 0.05% Tween 20, pH 7.4, further improved protein purity levels. Using Escherichia coli cell lysates expressing 90 recombinant Streptococcus pneumoniae proteins, 73 proteins were successfully immobilized, and 66 proteins were in situ purified with greater than 90% purity. We identified several antigens among the in situ-purified proteins via assays with anti-S. pneumoniae rabbit antibodies and a human patient antiserum, as a demonstration project of large scale microarray-based immunoproteomics profiling. The methodology is compatible with higher throughput formats of in vivo protein expression, eliminates the need for resin-based purification and circumvents

  3. Metabolism of minor isoforms of prion proteins: Cytosolic prion protein and transmembrane prion protein

    OpenAIRE

    Song, Zhiqi; Zhao, Deming; Yang, Lifeng

    2013-01-01

    Transmissible spongiform encephalopathy or prion disease is triggered by the conversion from cellular prion protein to pathogenic prion protein. Growing evidence has concentrated on prion protein configuration changes and their correlation with prion disease transmissibility and pathogenicity. In vivo and in vitro studies have shown that several cytosolic forms of prion protein with specific topological structure can destroy intracellular stability and contribute to prion protein pathogenicit...

  4. Predicting multiplex subcellular localization of proteins using protein-protein interaction network: a comparative study

    OpenAIRE

    Jiang Jonathan Q; Wu Maoying

    2012-01-01

    Abstract Background Proteins that interact in vivo tend to reside within the same or "adjacent" subcellular compartments. This observation provides opportunities to reveal protein subcellular localization in the context of the protein-protein interaction (PPI) network. However, so far, only a few efforts based on heuristic rules have been made in this regard. Results We systematically and quantitatively validate the hypothesis that proteins physically interacting with each other probably shar...

  5. Dairy Proteins and Energy Balance

    DEFF Research Database (Denmark)

    Bendtsen, Line Quist

    High protein diets affect energy balance beneficially through decreased hunger, enhanced satiety and increased energy expenditure. Dairy products are a major source of protein. Dairy proteins are comprised of two classes, casein (80%) and whey proteins (20%), which are both of high quality......, but casein is absorbed slowly and whey is absorbed rapidly. The present PhD study investigated the effects of total dairy proteins, whey, and casein, on energy balance and the mechanisms behind any differences in the effects of the specific proteins. The results do not support the hypothesis that dairy...... proteins, whey or casein are more beneficial than other protein sources in the regulation of energy balance, and suggest that dairy proteins, whey or casein seem to play only a minor role, if any, in the prevention and treatment of obesity....

  6. Coevolution study of mitochondria respiratory chain proteins:Toward the understanding of protein-protein interaction

    Institute of Scientific and Technical Information of China (English)

    Ming Yang; Yan Ge; Jiayan Wu; Jingfa Xiao; Jun Yu

    2011-01-01

    Coevolution can be seen as the interdependency between evolutionary histories. In the context of protein evolution, functional correlation proteins are ever-present coordinated evolutionary characters without disruption of organismal integrity. As to complex system, there are two forms of protein-protein interactions in vivo, which refer to inter-complex interaction and intra-complex interaction. In this paper, we studied the difference of coevolution characters between inter-complex interaction and intra-complex interaction using "Mirror tree" method on the respiratory chain (RC) proteins. We divided the correlation coefficients of every pairwise RC proteins into two groups corresponding to the binary protein-protein interaction in intra-complex and the binary protein-protein interaction in inter-complex, respectively. A dramatical discrepancy is detected between the coevolution characters of the two sets of protein interactions (Wilcoxon test, p-value = 4.4 x 10-6). Our finding reveals some critical information on coevolutionary study and assists the mechanical investigation of protein-protein interaction.Furthermore, the results also provide some unique clue for supramolecular organization of protein complexes in the mitochondrial inner membrane. More detailed binding sites map and genome information of nuclear encoded RC proteins will be extraordinary valuable for the further mitochondria dynamics study.

  7. Protein-protein interaction databases: keeping up with growing interactomes

    Directory of Open Access Journals (Sweden)

    Lehne Benjamin

    2009-04-01

    Full Text Available Abstract Over the past few years, the number of known protein-protein interactions has increased substantially. To make this information more readily available, a number of publicly available databases have set out to collect and store protein-protein interaction data. Protein-protein interactions have been retrieved from six major databases, integrated and the results compared. The six databases (the Biological General Repository for Interaction Datasets [BioGRID], the Molecular INTeraction database [MINT], the Biomolecular Interaction Network Database [BIND], the Database of Interacting Proteins [DIP], the IntAct molecular interaction database [IntAct] and the Human Protein Reference Database [HPRD] differ in scope and content; integration of all datasets is non-trivial owing to differences in data annotation. With respect to human protein-protein interaction data, HPRD seems to be the most comprehensive. To obtain a complete dataset, however, interactions from all six databases have to be combined. To overcome this limitation, meta-databases such as the Agile Protein Interaction Database (APID offer access to integrated protein-protein interaction datasets, although these also currently have certain restrictions.

  8. Mapping the human protein interactome

    Institute of Scientific and Technical Information of China (English)

    Daniel Figeys

    2008-01-01

    Interactions are the essence of all biomolecules because they cannot fulfill their roles without interacting with other molecules. Hence, mapping the interactions of biomolecules can be useful for understanding their roles and functions. Furthermore, the development of molecular based systems biology requires an understanding of the biomolecular interactions. In recent years, the mapping of protein-protein interactions in different species has been reported, but few reports have focused on the large-scale mapping of protein-protein interactions in human. Here, we review the developments in protein interaction mapping and we discuss issues and strategies for the mapping of the human protein interactome.

  9. Hydrogels Constructed from Engineered Proteins.

    Science.gov (United States)

    Li, Hongbin; Kong, Na; Laver, Bryce; Liu, Junqiu

    2016-02-24

    Due to their various potential biomedical applications, hydrogels based on engineered proteins have attracted considerable interest. Benefitting from significant progress in recombinant DNA technology and protein engineering/design techniques, the field of protein hydrogels has made amazing progress. The latest progress of hydrogels constructed from engineered recombinant proteins are presented, mainly focused on biorecognition-driven physical hydrogels as well as chemically crosslinked hydrogels. The various bio-recognition based physical crosslinking strategies are discussed, as well as chemical crosslinking chemistries used to engineer protein hydrogels, and protein hydrogels' various biomedical applications. The future perspectives of this fast evolving field of biomaterials are also discussed.

  10. Hydrogels Constructed from Engineered Proteins.

    Science.gov (United States)

    Li, Hongbin; Kong, Na; Laver, Bryce; Liu, Junqiu

    2016-02-24

    Due to their various potential biomedical applications, hydrogels based on engineered proteins have attracted considerable interest. Benefitting from significant progress in recombinant DNA technology and protein engineering/design techniques, the field of protein hydrogels has made amazing progress. The latest progress of hydrogels constructed from engineered recombinant proteins are presented, mainly focused on biorecognition-driven physical hydrogels as well as chemically crosslinked hydrogels. The various bio-recognition based physical crosslinking strategies are discussed, as well as chemical crosslinking chemistries used to engineer protein hydrogels, and protein hydrogels' various biomedical applications. The future perspectives of this fast evolving field of biomaterials are also discussed. PMID:26707834

  11. Motif-Driven Design of Protein-Protein Interfaces.

    Science.gov (United States)

    Silva, Daniel-Adriano; Correia, Bruno E; Procko, Erik

    2016-01-01

    Protein-protein interfaces regulate many critical processes for cellular function. The ability to accurately control and regulate these molecular interactions is of major interest for biomedical and synthetic biology applications, as well as to address fundamental biological questions. In recent years, computational protein design has emerged as a tool for designing novel protein-protein interactions with functional relevance. Although attractive, these computational tools carry a steep learning curve. In order to make some of these methods more accessible, we present detailed descriptions and examples of ROSETTA computational protocols for the design of functional protein binders using seeded protein interface design. In these protocols, a motif of known structure that interacts with the target site is grafted into a scaffold protein, followed by design of the surrounding interaction surface. PMID:27094298

  12. Competitive Protein Adsorption - Multilayer Adsorption and Surface Induced Protein Aggregation

    DEFF Research Database (Denmark)

    Holmberg, Maria; Hou, Xiaolin

    2009-01-01

    In this study, competitive adsorption of albumin and IgG (immunoglobulin G) from human serum solutions and protein mixtures onto polymer surfaces is studied by means of radioactive labeling. By using two different radiolabels (125I and 131I), albumin and IgG adsorption to polymer surfaces...... is monitored simultaneously and the influence from the presence of other human serum proteins on albumin and IgG adsorption, as well as their mutual influence during adsorption processes, is investigated. Exploring protein adsorption by combining analysis of competitive adsorption from complex solutions...... of high concentration with investigation of single protein adsorption and interdependent adsorption between two specific proteins enables us to map protein adsorption sequences during competitive protein adsorption. Our study shows that proteins can adsorb in a multilayer fashion onto the polymer surfaces...

  13. How do oncoprotein mutations rewire protein-protein interaction networks?

    Science.gov (United States)

    Bowler, Emily H; Wang, Zhenghe; Ewing, Rob M

    2015-01-01

    The acquisition of mutations that activate oncogenes or inactivate tumor suppressors is a primary feature of most cancers. Mutations that directly alter protein sequence and structure drive the development of tumors through aberrant expression and modification of proteins, in many cases directly impacting components of signal transduction pathways and cellular architecture. Cancer-associated mutations may have direct or indirect effects on proteins and their interactions and while the effects of mutations on signaling pathways have been widely studied, how mutations alter underlying protein-protein interaction networks is much less well understood. Systematic mapping of oncoprotein protein interactions using proteomics techniques as well as computational network analyses is revealing how oncoprotein mutations perturb protein-protein interaction networks and drive the cancer phenotype. PMID:26325016

  14. Protein-protein interactions in DNA mismatch repair.

    Science.gov (United States)

    Friedhoff, Peter; Li, Pingping; Gotthardt, Julia

    2016-02-01

    The principal DNA mismatch repair proteins MutS and MutL are versatile enzymes that couple DNA mismatch or damage recognition to other cellular processes. Besides interaction with their DNA substrates this involves transient interactions with other proteins which is triggered by the DNA mismatch or damage and controlled by conformational changes. Both MutS and MutL proteins have ATPase activity, which adds another level to control their activity and interactions with DNA substrates and other proteins. Here we focus on the protein-protein interactions, protein interaction sites and the different levels of structural knowledge about the protein complexes formed with MutS and MutL during the mismatch repair reaction. PMID:26725162

  15. A magnetic protein biocompass

    Science.gov (United States)

    Qin, Siying; Yin, Hang; Yang, Celi; Dou, Yunfeng; Liu, Zhongmin; Zhang, Peng; Yu, He; Huang, Yulong; Feng, Jing; Hao, Junfeng; Hao, Jia; Deng, Lizong; Yan, Xiyun; Dong, Xiaoli; Zhao, Zhongxian; Jiang, Taijiao; Wang, Hong-Wei; Luo, Shu-Jin; Xie, Can

    2016-02-01

    The notion that animals can detect the Earth’s magnetic field was once ridiculed, but is now well established. Yet the biological nature of such magnetosensing phenomenon remains unknown. Here, we report a putative magnetic receptor (Drosophila CG8198, here named MagR) and a multimeric magnetosensing rod-like protein complex, identified by theoretical postulation and genome-wide screening, and validated with cellular, biochemical, structural and biophysical methods. The magnetosensing complex consists of the identified putative magnetoreceptor and known magnetoreception-related photoreceptor cryptochromes (Cry), has the attributes of both Cry- and iron-based systems, and exhibits spontaneous alignment in magnetic fields, including that of the Earth. Such a protein complex may form the basis of magnetoreception in animals, and may lead to applications across multiple fields.

  16. Cow's Milk Protein Allergy.

    Science.gov (United States)

    Mousan, Grace; Kamat, Deepak

    2016-10-01

    Cow's milk protein allergy (CMPA) is a common condition encountered in children with incidence estimated as 2% to 7.5% in the first year of life. Formula and breast-fed babies can present with symptoms of CMPA. It is important to accurately diagnose CMPA to avoid the consequences of either under- or overdiagnosis. CMPA is classically categorized into immunoglobulin E (IgE)- or non-IgE-mediated reaction that vary in clinical manifestations, diagnostic evaluation, and prognosis. The most commonly involved systems in patients with CMPA are gastrointestinal, skin, and respiratory. Evaluation of CMPA starts with good data gathering followed by testing if indicated. Treatment is simply by avoidance of cow's milk protein (CMP) in the child's or mother's diet, if exclusively breast-feeding. This article reviews the definition, epidemiology, risk factors, pathogenesis, clinical presentation, evaluation, management, and prognosis of CMPA and provides an overview of different options for formulas and their indication in the treatment of CMPA.

  17. Protein Functionalized Nanodiamond Arrays

    Directory of Open Access Journals (Sweden)

    Liu YL

    2010-01-01

    Full Text Available Abstract Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli.

  18. Neurocognitive derivation of protein surface property from protein aggregate parameters

    OpenAIRE

    Mishra, Hrishikesh; Lahiri, Tapobrata

    2011-01-01

    Current work targeted to predicate parametric relationship between aggregate and individual property of a protein. In this approach, we considered individual property of a protein as its Surface Roughness Index (SRI) which was shown to have potential to classify SCOP protein families. The bulk property was however considered as Intensity Level based Multi-fractal Dimension (ILMFD) of ordinary microscopic images of heat denatured protein aggregates which was known to have potential to serve as...

  19. Biophysics of protein evolution and evolutionary protein biophysics

    OpenAIRE

    Sikosek, Tobias; Chan, Hue Sun

    2014-01-01

    The study of molecular evolution at the level of protein-coding genes often entails comparing large datasets of sequences to infer their evolutionary relationships. Despite the importance of a protein's structure and conformational dynamics to its function and thus its fitness, common phylogenetic methods embody minimal biophysical knowledge of proteins. To underscore the biophysical constraints on natural selection, we survey effects of protein mutations, highlighting the physical basis for ...

  20. Hub Promiscuity in Protein-Protein Interaction Networks

    OpenAIRE

    Haruki Nakamura; Kengo Kinoshita; Ashwini Patil

    2010-01-01

    Hubs are proteins with a large number of interactions in a protein-protein interaction network. They are the principal agents in the interaction network and affect its function and stability. Their specific recognition of many different protein partners is of great interest from the structural viewpoint. Over the last few years, the structural properties of hubs have been extensively studied. We review the currently known features that are particular to hubs, possibly affecting their binding ...

  1. Geometric De-noising of Protein-Protein Interaction Networks

    OpenAIRE

    Kuchaiev, Oleksii; Rasajski, Marija; Higham, Desmond J.; Przul, Natasa; Przytycka, Teresa Maria

    2009-01-01

    Understanding complex networks of protein-protein interactions (PPIs) is one of the foremost challenges of the post-genomic era. Due to the recent advances in experimental bio-technology, including yeast-2-hybrid (Y2H), tandem affinity purification (TAP) and other high-throughput methods for protein-protein interaction (PPI) detection, huge amounts of PPI network data are becoming available. Of major concern, however, are the levels of noise and incompleteness. For example, for Y2H screens, i...

  2. Statistical thermodynamics of membrane bending mediated protein-protein attraction

    OpenAIRE

    Chou, Tom; Kim, Ken S.; Oster, George

    1999-01-01

    Integral membrane proteins deform the surrounding bilayer creating long-ranged forces that influence distant proteins. These forces can be attractive or repulsive, depending on the proteins' shape, height, contact angle with the bilayer, as well as the local membrane curvature. Although interaction energies are not pairwise additive, for sufficiently low protein density, thermodynamic properties depend only upon pair interactions. Here, we compute pair interaction potentials and entropic cont...

  3. Protein-protein fusion catalyzed by sortase A.

    Science.gov (United States)

    Levary, David A; Parthasarathy, Ranganath; Boder, Eric T; Ackerman, Margaret E

    2011-04-06

    Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality--demonstrating the robust and facile nature of this reaction.

  4. Protein-protein fusion catalyzed by sortase A.

    Directory of Open Access Journals (Sweden)

    David A Levary

    Full Text Available Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality--demonstrating the robust and facile nature of this reaction.

  5. Metabolism of minor isoforms of prion proteins Cytosolic prion protein and transmembrane prion protein*

    Institute of Scientific and Technical Information of China (English)

    Zhiqi Song; Deming Zhao; Lifeng Yang

    2013-01-01

    Transmissible spongiform encephalopathy or prion disease is triggered by the conversion from cellular prion protein to pathogenic prion protein. Growing evidence has concentrated on prion protein configuration changes and their correlation with prion disease transmissibility and pathoge-nicity. In vivo and in vitro studies have shown that several cytosolic forms of prion protein with spe-cific topological structure can destroy intracellular stability and contribute to prion protein pathoge-nicity. In this study, the latest molecular chaperone system associated with endoplasmic reticu-lum-associated protein degradation, the endoplasmic reticulum resident protein quality-control system and the ubiquitination proteasome system, is outlined. The molecular chaperone system directly correlates with the prion protein degradation pathway. Understanding the molecular me-chanisms wil help provide a fascinating avenue for further investigations on prion disease treatment and prion protein-induced neurodegenerative diseases.

  6. HRTEM in protein crystallography

    International Nuclear Information System (INIS)

    Electron microscopy/diffraction (ED/D) using spot-scan and low-dose imaging has been successfully applied to investigate microcrystals of an alpha-helical coiled-coil protein extracted from ootheca of the praying mantis. Fourier transforms of the images show resolution out to 4 Angstroems and can be used to phase the corresponding ED data which shows reflections out to 2 Aangstroems. 5 refs., 3 figs

  7. Tuber Storage Proteins

    OpenAIRE

    Shewry, Peter R.

    2003-01-01

    A wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. These are potato (Solanum tuberosum), cassava (Manihot esculenta), sweet potato (Ipomoea batatus), yams (Dioscorea spp.) and taro (Colocasia, Cyrtosperma and Xanthosoma spp.). All of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. Thus, patatin from potato exhibits act...

  8. Neutron protein crystallography

    Energy Technology Data Exchange (ETDEWEB)

    Niimura, Nobuo [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-10-01

    X-ray diffraction of single crystal has enriched the knowledge of various biological molecules such as proteins, DNA, t-RNA, viruses, etc. It is difficult to make structural analysis of hydrogen atoms in a protein using X-ray crystallography, whereas neutron diffraction seems usable to directly determine the location of those hydrogen atoms. Here, neutron diffraction method was applied to structural analysis of hen egg-white lysozyme. Since the crystal size of a protein to analyze is generally small (5 mm{sup 3} at most), the neutron beam at the sample position in monochromator system was set to less than 5 x 5 mm{sup 2} and beam divergence to 0.4 degree or less. Neutron imaging plate with {sup 6}Li or Gd mixed with photostimulated luminescence material was used and about 2500 Bragg reflections were recorded in one crystal setting. A total of 38278 reflections for 2.0 A resolution were collected in less than 10 days. Thus, stereo views of Trp-111 omit map around the indol ring of Trp-111 was presented and the three-dimensional arrangement of 696H and 264D atoms in the lysozyme molecules was determined using the omit map. (M.N.)

  9. Hydrolyzed Proteins in Allergy.

    Science.gov (United States)

    Salvatore, Silvia; Vandenplas, Yvan

    2016-01-01

    Hydrolyzed proteins are used worldwide in the therapeutic management of infants with allergic manifestations and have long been proposed as a dietetic measure to prevent allergy in at risk infants. The degree and method of hydrolysis, protein source and non-nitrogen components characterize different hydrolyzed formulas (HFs) and may determine clinical efficacy, tolerance and nutritional effects. Cow's milk (CM)-based HFs are classified as extensively (eHF) or partially HF (pHF) based on the percentage of small peptides. One whey pHF has been shown to reduce atopic dermatitis in high-risk infants who are not exclusively breastfed. More studies are needed to determine the benefit of these formulas in the prevention of CM allergy (CMA) and in the general population. eHFs represent up to now the treatment of choice for most infants with CMA. However, new developments, such as an extensively hydrolyzed rice protein-based formula, could become alternative options if safety and nutritional and therapeutic efficacy are confirmed as this type of formula is less expensive. In some countries, an extensive soy hydrolysate is available. PMID:27336625

  10. Ontology integration to identify protein complex in protein interaction networks

    Directory of Open Access Journals (Sweden)

    Yang Zhihao

    2011-10-01

    Full Text Available Abstract Background Protein complexes can be identified from the protein interaction networks derived from experimental data sets. However, these analyses are challenging because of the presence of unreliable interactions and the complex connectivity of the network. The integration of protein-protein interactions with the data from other sources can be leveraged for improving the effectiveness of protein complexes detection algorithms. Methods We have developed novel semantic similarity method, which use Gene Ontology (GO annotations to measure the reliability of protein-protein interactions. The protein interaction networks can be converted into a weighted graph representation by assigning the reliability values to each interaction as a weight. Following the approach of that of the previously proposed clustering algorithm IPCA which expands clusters starting from seeded vertices, we present a clustering algorithm OIIP based on the new weighted Protein-Protein interaction networks for identifying protein complexes. Results The algorithm OIIP is applied to the protein interaction network of Sacchromyces cerevisiae and identifies many well known complexes. Experimental results show that the algorithm OIIP has higher F-measure and accuracy compared to other competing approaches.

  11. Yeast Interacting Proteins Database: YJL199C, YJL199C [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available d in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies...cies; protein detected in large-scale protein-protein interaction studies Rows with this prey as prey (4) Ro...n; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies... species; protein detected in large-scale protein-protein interaction studies Rows with this prey as prey Ro

  12. Protein: FEA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEA3 AREB pathway: AREB transcription factors ABF2 AREB1, BZIP36 ABSCISIC ACID-INSE...NSITIVE 5-like protein 5 ABA-responsive element-binding protein 1, Abscisic acid responsive elements-binding

  13. Protein: FEA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FEA3 AREB pathway: AREB transcription factors ABF4 AREB2, BZIP38 ABSCISIC ACID-INSE...NSITIVE 5-like protein 7 ABA-responsive element-binding protein 2, Abscisic acid responsive elements-binding

  14. Controlling allosteric networks in proteins

    Science.gov (United States)

    Dokholyan, Nikolay

    2013-03-01

    We present a novel methodology based on graph theory and discrete molecular dynamics simulations for delineating allosteric pathways in proteins. We use this methodology to uncover the structural mechanisms responsible for coupling of distal sites on proteins and utilize it for allosteric modulation of proteins. We will present examples where inference of allosteric networks and its rewiring allows us to ``rescue'' cystic fibrosis transmembrane conductance regulator (CFTR), a protein associated with fatal genetic disease cystic fibrosis. We also use our methodology to control protein function allosterically. We design a novel protein domain that can be inserted into identified allosteric site of target protein. Using a drug that binds to our domain, we alter the function of the target protein. We successfully tested this methodology in vitro, in living cells and in zebrafish. We further demonstrate transferability of our allosteric modulation methodology to other systems and extend it to become ligh-activatable.

  15. Microtubules, Tubulins and Associated Proteins.

    Science.gov (United States)

    Raxworthy, Michael J.

    1988-01-01

    Reviews much of what is known about microtubules, which are biopolymers consisting predominantly of subunits of the globular protein, tubulin. Describes the functions of microtubules, their structure and assembly, microtube associated proteins, and microtubule-disrupting agents. (TW)

  16. Protein: FBA6 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA6 transport vesicle formation SEC12 SED2 Guanine nucleotide-exchange factor SEC12 Protein... transport protein SEC12 559292 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 855760 P11655 ...

  17. Protein: FBA4 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA4 general transcription factor TFIIH CDK8 CDK8 Cyclin-dependent kinase 8 Cell division protein... kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein

  18. Protein Linked to Atopic Dermatitis

    Science.gov (United States)

    ... Research Matters NIH Research Matters January 14, 2013 Protein Linked to Atopic Dermatitis Normal skin from a ... in mice suggests that lack of a certain protein may trigger atopic dermatitis, the most common type ...

  19. Stabilized polyacrylic saccharide protein conjugates

    Science.gov (United States)

    Callstrom, M.R.; Bednarski, M.D.; Gruber, P.R.

    1996-02-20

    This invention is directed to water soluble protein polymer conjugates which are stable in hostile environments. The conjugate comprises a protein which is linked to an acrylic polymer at multiple points through saccharide linker groups. 16 figs.

  20. Functional aspects of protein flexibility

    DEFF Research Database (Denmark)

    Teilum, Kaare; Olsen, Johan G; Kragelund, Birthe B

    2009-01-01

    Proteins are dynamic entities, and they possess an inherent flexibility that allows them to function through molecular interactions within the cell, among cells and even between organisms. Appreciation of the non-static nature of proteins is emerging, but to describe and incorporate...... this into an intuitive perception of protein function is challenging. Flexibility is of overwhelming importance for protein function, and the changes in protein structure during interactions with binding partners can be dramatic. The present review addresses protein flexibility, focusing on protein-ligand interactions....... The thermodynamics involved are reviewed, and examples of structure-function studies involving experimentally determined flexibility descriptions are presented. While much remains to be understood about protein flexibility, it is clear that it is encoded within their amino acid sequence and should be viewed...

  1. Protein kinase substrate identification on functional protein arrays

    Directory of Open Access Journals (Sweden)

    Zhou Fang

    2008-02-01

    Full Text Available Abstract Background Over the last decade, kinases have emerged as attractive therapeutic targets for a number of different diseases, and numerous high throughput screening efforts in the pharmaceutical community are directed towards discovery of compounds that regulate kinase function. The emerging utility of systems biology approaches has necessitated the development of multiplex tools suitable for proteomic-scale experiments to replace lower throughput technologies such as mass spectroscopy for the study of protein phosphorylation. Recently, a new approach for identifying substrates of protein kinases has applied the miniaturized format of functional protein arrays to characterize phosphorylation for thousands of candidate protein substrates in a single experiment. This method involves the addition of protein kinases in solution to arrays of immobilized proteins to identify substrates using highly sensitive radioactive detection and hit identification algorithms. Results To date, the factors required for optimal performance of protein array-based kinase substrate identification have not been described. In the current study, we have carried out a detailed characterization of the protein array-based method for kinase substrate identification, including an examination of the effects of time, buffer compositions, and protein concentration on the results. The protein array approach was compared to standard solution-based assays for assessing substrate phosphorylation, and a correlation of greater than 80% was observed. The results presented here demonstrate how novel substrates for protein kinases can be quickly identified from arrays containing thousands of human proteins to provide new clues to protein kinase function. In addition, a pooling-deconvolution strategy was developed and applied that enhances characterization of specific kinase-substrate relationships and decreases reagent consumption. Conclusion Functional protein microarrays are an

  2. Protein: MPA1 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available MPA1 TLR signaling molecules Nlrp3 Cias1, Mmig1, Nalp3, Pypaf1 NACHT, LRR and PYD domains-containing protein... 3 Cold autoinflammatory syndrome 1 protein homolog, Cryopyrin, Mast cell maturatio...n-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1 10090 Mus musculus 216799 Q8R4B8 Q8R4B8 20007575 ...

  3. Green fluorescent protein: A perspective

    OpenAIRE

    Remington, S James

    2011-01-01

    A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure-function relationship...

  4. Recent advances of protein microarrays

    OpenAIRE

    Hultschig, Claus; Kreutzberger, Jürgen; Seitz, Harald; Konthur, Zoltán; Büssow, Konrad; Lehrach, Hans

    2006-01-01

    Technological innovations and novel applications have greatly advanced the field of protein microarrays. Over the past two years, different types of protein microarrays have been used for serum profiling, protein abundance determinations, and identification of proteins that bind DNA or small compounds. However, considerable development is still required to ensure common quality standards and to establish large content repertoires. Here, we summarize applications available to date and discuss ...

  5. Similarity measures for protein ensembles

    DEFF Research Database (Denmark)

    Lindorff-Larsen, Kresten; Ferkinghoff-Borg, Jesper

    2009-01-01

    Analyses of similarities and changes in protein conformation can provide important information regarding protein function and evolution. Many scores, including the commonly used root mean square deviation, have therefore been developed to quantify the similarities of different protein conformations...... a synthetic example from molecular dynamics simulations. We then apply the algorithms to revisit the problem of ensemble averaging during structure determination of proteins, and find that an ensemble refinement method is able to recover the correct distribution of conformations better than standard single...

  6. Spectral affinity in protein networks

    OpenAIRE

    Teng Shang-Hua; Voevodski Konstantin; Xia Yu

    2009-01-01

    Abstract Background Protein-protein interaction (PPI) networks enable us to better understand the functional organization of the proteome. We can learn a lot about a particular protein by querying its neighborhood in a PPI network to find proteins with similar function. A spectral approach that considers random walks between nodes of interest is particularly useful in evaluating closeness in PPI networks. Spectral measures of closeness are more robust to noise in the data and are more precise...

  7. Charge configurations in viral proteins.

    OpenAIRE

    Karlin, S; Brendel, V

    1988-01-01

    The spatial distribution of the charged residues of a protein is of interest with respect to potential electrostatic interactions. We have examined the proteins of a large number of representative eukaryotic and prokaryotic viruses for the occurrence of significant clusters, runs, and periodic patterns of charge. Clusters and runs of positive charge are prominent in many capsid and core proteins, whereas surface (glyco)proteins frequently contain a negative charge cluster. Significant charge ...

  8. High throughput recombinant protein production of fungal secreted proteins

    DEFF Research Database (Denmark)

    Vala, Andrea Lages Lino; Roth, Doris; Grell, Morten Nedergaard;

    2011-01-01

    Secreted proteins are important for both symbiotic and pathogenic interactions between fungi and their hosts. Our research group uses screens and genomic mining to discover novel proteins involved in these processes. To efficiently study the large number of candidate proteins, we are establishing...... soluble proteins are then produced in larger quantities, purified and assayed for new enzymatic activities. We used transposon-assisted signal sequence trapping (TAST) to identify putative secreted proteins expressed during the interactions between the basidiomycete Paxillus involutus and birch (symbiotic...

  9. Functional Foods Containing Whey Proteins

    Science.gov (United States)

    Whey proteins, modified whey proteins, and whey components are useful as nutrients or supplements for health maintenance. Extrusion modified whey proteins can easily fit into new products such as beverages, confectionery items (e.g., candies), convenience foods, desserts, baked goods, sauces, and in...

  10. Protein: FBA4 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA4 REST-TBP TBP GTF2D1, TF2D, TFIID TATA_binding_protein TATA-box-binding protein... TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID

  11. Protein: FBA4 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available tion factor complex helicase XPB subunit Basic transcription factor 2 89 kDa subunit, DNA excision repair protein... ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa s...ubunit, Xeroderma pigmentosum group B-complementing protein 9606 Homo sapiens P19447 2071 2071 P19447 ...

  12. BINDING ISOTHERMS SURFACTANT-PROTEINS

    OpenAIRE

    Elena Irina Moater; Cristiana Radulescu; Ionica Ionita

    2011-01-01

    The interactions between surfactants and proteins shows some similarities with interactions between surfactants and polymers, but the hydrophobic amphoteric nature of proteins and their secondary and tertiary structure components make them different from conventional polymer systems. Many studies from the past about surfactant - proteins bonding used the dialysis techniques. Other techniques used to determine the binding isotherm, included ultrafiltration, ultracentrifugation, potentiometry, ...

  13. Structuring high-protein foods

    NARCIS (Netherlands)

    Purwanti, N.

    2012-01-01

    Increased protein consumption gives rise to various health benefits. High-protein intake can lead to muscle development, body weight control and suppression of sarcopenia progression. However, increasing the protein content in food products leads to textural changes over time. These changes result i

  14. Hydrophobic patches on protein surfaces

    NARCIS (Netherlands)

    Lijnzaad, P.

    2007-01-01

    Hydrophobicity is a prime determinant of the structure and function of proteins. It is the driving force behind the folding of soluble proteins, and when exposed on the surface, it is frequently involved in recognition and binding of ligands and other proteins. The energetic cost of exposing hydroph

  15. Validation of protein carbonyl measurement

    DEFF Research Database (Denmark)

    Augustyniak, Edyta; Adam, Aisha; Wojdyla, Katarzyna;

    2015-01-01

    Protein carbonyls are widely analysed as a measure of protein oxidation. Several different methods exist for their determination. A previous study had described orders of magnitude variance that existed when protein carbonyls were analysed in a single laboratory by ELISA using different commercial...

  16. Transient interactions between photosynthetic proteins

    NARCIS (Netherlands)

    Hulsker, Rinske

    2008-01-01

    The biological processes that are the basis of all life forms are mediated largely by protein-protein interactions. The protein complexes involved in these interactions can be categorised by their affinity, which results in a range from static to transient complexes. Electron transfer complexes, whi

  17. Protein stress and stress proteins: implications in aging and disease

    Indian Academy of Sciences (India)

    C Sőti; Péter Csermely

    2007-04-01

    Environmantal stress induces damage that activates an adaptive response in any organism. The cellular stress response is based on the induction of cytoprotective proteins, the so called stress or heat shock proteins. The stress response as well as stress proteins are ubiquitous, highly conserved mechanism, and genes, respectively, already present in prokaryotes. Chaperones protect the proteome against conformational damage, promoting the function of protein networks. Protein damage takes place during aging and in several degenerative diseases, and presents a threat to overload the cellular defense mechanisms. The preservation of a robust stress response and protein disposal is indispensable for health and longevity. This review summarizes the present knowledge of protein damage, turnover, and the stress response in aging and degenerative diseases.

  18. Flowering Buds of Globular Proteins: Transpiring Simplicity of Protein Organization

    Science.gov (United States)

    Berezovsky, Igor N.

    2002-01-01

    Structural and functional complexity of proteins is dramatically reduced to a simple linear picture when the laws of polymer physics are considered. A basic unit of the protein structure is a nearly standard closed loop of 25–35 amino acid residues, and every globular protein is built of consecutively connected closed loops. The physical necessity of the closed loops had been apparently imposed on the early stages of protein evolution. Indeed, the most frequent prototype sequence motifs in prokaryotic proteins have the same sequence size, and their high match representatives are found as closed loops in crystallized proteins. Thus, the linear organization of the closed loop elements is a quintessence of protein evolution, structure and folding. PMID:18629251

  19. Protein subcellular localization assays using split fluorescent proteins

    Science.gov (United States)

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2009-09-08

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  20. Protein enriched pasta: structure and digestibility of its protein network.

    Science.gov (United States)

    Laleg, Karima; Barron, Cécile; Santé-Lhoutellier, Véronique; Walrand, Stéphane; Micard, Valérie

    2016-02-01

    Wheat (W) pasta was enriched in 6% gluten (G), 35% faba (F) or 5% egg (E) to increase its protein content (13% to 17%). The impact of the enrichment on the multiscale structure of the pasta and on in vitro protein digestibility was studied. Increasing the protein content (W- vs. G-pasta) strengthened pasta structure at molecular and macroscopic scales but reduced its protein digestibility by 3% by forming a higher covalently linked protein network. Greater changes in the macroscopic and molecular structure of the pasta were obtained by varying the nature of protein used for enrichment. Proteins in G- and E-pasta were highly covalently linked (28-32%) resulting in a strong pasta structure. Conversely, F-protein (98% SDS-soluble) altered the pasta structure by diluting gluten and formed a weak protein network (18% covalent link). As a result, protein digestibility in F-pasta was significantly higher (46%) than in E- (44%) and G-pasta (39%). The effect of low (55 °C, LT) vs. very high temperature (90 °C, VHT) drying on the protein network structure and digestibility was shown to cause greater molecular changes than pasta formulation. Whatever the pasta, a general strengthening of its structure, a 33% to 47% increase in covalently linked proteins and a higher β-sheet structure were observed. However, these structural differences were evened out after the pasta was cooked, resulting in identical protein digestibility in LT and VHT pasta. Even after VHT drying, F-pasta had the best amino acid profile with the highest protein digestibility, proof of its nutritional interest.

  1. Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer

    Science.gov (United States)

    Koshiba, Takumi

    2015-01-01

    Protein-protein interactions are essential biological reactions occurring at inter- and intra-cellular levels. The analysis of their mechanism is generally required in order link to understand their various cellular functions. Bioluminescence resonance energy transfer (BRET), which is based on an enzymatic activity of luciferase, is a useful tool for investigating protein-protein interactions in live cells. The combination of the BRET system and biomolecular fluorescence complementation (BiFC) would provide us a better understanding of the hetero-oligomeric structural states of protein complexes. In this review, we discuss the application of BRET to the protein-protein interactions of mitochondrial-associated proteins and discuss its physiological relevance. PMID:27493852

  2. CPL:Detecting Protein Complexes by Propagating Labels on Protein-Protein Interaction Network

    Institute of Scientific and Technical Information of China (English)

    代启国; 郭茂祖; 刘晓燕; 滕志霞; 王春宇

    2014-01-01

    Proteins usually bind together to form complexes, which play an important role in cellular activities. Many graph clustering methods have been proposed to identify protein complexes by finding dense regions in protein-protein interaction networks. We present a novel framework (CPL) that detects protein complexes by propagating labels through interactions in a network, in which labels denote complex identifiers. With proper propagation in CPL, proteins in the same complex will be assigned with the same labels. CPL does not make any strong assumptions about the topological structures of the complexes, as in previous methods. The CPL algorithm is tested on several publicly available yeast protein-protein interaction networks and compared with several state-of-the-art methods. The results suggest that CPL performs better than the existing methods. An analysis of the functional homogeneity based on a gene ontology analysis shows that the detected complexes of CPL are highly biologically relevant.

  3. Protein from methanol

    Energy Technology Data Exchange (ETDEWEB)

    Rosenzweig, M.; Ushio, S.

    1974-01-07

    The biosynthesis of proteins from methanol produced from natural gas can provide an attractive alternative to the already commercially proven technique of protein synthesis from gas oil and n-paraffin feedstocks if current pilot-plant tests in England and Japan prove successful. The methanol route also provides other advantages as a protein feedstock: it is water soluble, contains no polycyclic aromatic compounds, and requires less oxygen than methane. Its lower boiling point helps ease the separation of feedstock from the product stream. Finally, it will require lower investment costs. Both ICI and Mitsubishi Gas Chemical Co. are large methanol producers. ICI already has a 1000 ton/yr plant operating at Teeside, England, and expects to decide on a 100,000 m ton/yr plant later this year. Mitsubishi is constructing a large-scale pilot plant scheduled to come onstream this year. ICI will use a Pseudomona bacterium at 98.6/sup 0/F (37/sup 0/C) in the fermenter. Mitsubishi has not yet decided on a yeast or a bacteria, and is searching for a strain capable of withstanding up to 115/sup 0/F (46/sup 0/C). In the more advanced ICI process, methanol will be mixed with phosphoric acid, potassium sulfate, sodium chloride, and traces of iron, copper, zinc, and molybdenum; diluted with water; passed through a sterilization tank; and fermented at pH 7 in a pressure cycle fermenter. The product stream, containing a 3 percent suspension of cellular dry matter, is taken near the top of the fermenter riser, then passed through a flotation vessel and a centrifuge to pack the cell concentration to 20 percent. Water is recycled. Whatever methanol remains in the fermenter product stream is either used up by the microorganisms in subsequent processing or vaporized in the dryer. (auth)

  4. Discover Protein Complexes in Protein-Protein Interaction Networks Using Parametric Local Modularity

    OpenAIRE

    Tan Kai; Kim Jongkwang

    2010-01-01

    Abstract Background Recent advances in proteomic technologies have enabled us to create detailed protein-protein interaction maps in multiple species and in both normal and diseased cells. As the size of the interaction dataset increases, powerful computational methods are required in order to effectively distil network models from large-scale interactome data. Results We present an algorithm, miPALM (Module Inference by Parametric Local Modularity), to infer protein complexes in a protein-pr...

  5. HKC: An Algorithm to Predict Protein Complexes in Protein-Protein Interaction Networks

    OpenAIRE

    Xiaomin Wang; Zhengzhi Wang; Jun Ye

    2011-01-01

    With the availability of more and more genome-scale protein-protein interaction (PPI) networks, research interests gradually shift to Systematic Analysis on these large data sets. A key topic is to predict protein complexes in PPI networks by identifying clusters that are densely connected within themselves but sparsely connected with the rest of the network. In this paper, we present a new topology-based algorithm, HKC, to detect protein complexes in genome-scale PPI networks. HKC mainly use...

  6. Probabilistic methods for predicting protein functions in protein-protein interaction networks

    OpenAIRE

    Best, Christoph; Zimmer, Ralf; Apostolakis, Joannis

    2005-01-01

    We discuss probabilistic methods for predicting protein functions from protein-protein interaction networks. Previous work based on Markov Randon Fields is extended and compared to a general machine-learning theoretic approach. Using actual protein interaction networks for yeast from the MIPS database and GO-SLIM function assignments, we compare the predictions of the different probabilistic methods and of a standard support vector machine. It turns out that, with the currently available netw...

  7. Protein: FBA3 [TP Atlas

    Lifescience Database Archive (English)

    Full Text Available FBA3 Atg8 conjugation sysytem Map1lc3b Map1alc3, Map1lc3 MAP1LC3B Microtubule-associated protein...s 1A/1B light chain 3B Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifi...er LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 l

  8. High throughput protein-protein interaction data: clues for the architecture of protein complexes

    Directory of Open Access Journals (Sweden)

    Pang Chi

    2008-11-01

    Full Text Available Abstract Background High-throughput techniques are becoming widely used to study protein-protein interactions and protein complexes on a proteome-wide scale. Here we have explored the potential of these techniques to accurately determine the constituent proteins of complexes and their architecture within the complex. Results Two-dimensional representations of the 19S and 20S proteasome, mediator, and SAGA complexes were generated and overlaid with high quality pairwise interaction data, core-module-attachment classifications from affinity purifications of complexes and predicted domain-domain interactions. Pairwise interaction data could accurately determine the members of each complex, but was unexpectedly poor at deciphering the topology of proteins in complexes. Core and module data from affinity purification studies were less useful for accurately defining the member proteins of these complexes. However, these data gave strong information on the spatial proximity of many proteins. Predicted domain-domain interactions provided some insight into the topology of proteins within complexes, but was affected by a lack of available structural data for the co-activator complexes and the presence of shared domains in paralogous proteins. Conclusion The constituent proteins of complexes are likely to be determined with accuracy by combining data from high-throughput techniques. The topology of some proteins in the complexes will be able to be clearly inferred. We finally suggest strategies that can be employed to use high throughput interaction data to define the membership and understand the architecture of proteins in novel complexes.

  9. Human cancer protein-protein interaction network: a structural perspective.

    Directory of Open Access Journals (Sweden)

    Gozde Kar

    2009-12-01

    Full Text Available Protein-protein interaction networks provide a global picture of cellular function and biological processes. Some proteins act as hub proteins, highly connected to others, whereas some others have few interactions. The dysfunction of some interactions causes many diseases, including cancer. Proteins interact through their interfaces. Therefore, studying the interface properties of cancer-related proteins will help explain their role in the interaction networks. Similar or overlapping binding sites should be used repeatedly in single interface hub proteins, making them promiscuous. Alternatively, multi-interface hub proteins make use of several distinct binding sites to bind to different partners. We propose a methodology to integrate protein interfaces into cancer interaction networks (ciSPIN, cancer structural protein interface network. The interactions in the human protein interaction network are replaced by interfaces, coming from either known or predicted complexes. We provide a detailed analysis of cancer related human protein-protein interfaces and the topological properties of the cancer network. The results reveal that cancer-related proteins have smaller, more planar, more charged and less hydrophobic binding sites than non-cancer proteins, which may indicate low affinity and high specificity of the cancer-related interactions. We also classified the genes in ciSPIN according to phenotypes. Within phenotypes, for breast cancer, colorectal cancer and leukemia, interface properties were found to be discriminating from non-cancer interfaces with an accuracy of 71%, 67%, 61%, respectively. In addition, cancer-related proteins tend to interact with their partners through distinct interfaces, corresponding mostly to multi-interface hubs, which comprise 56% of cancer-related proteins, and constituting the nodes with higher essentiality in the network (76%. We illustrate the interface related affinity properties of two cancer-related hub

  10. Mathematical methods for protein science

    Energy Technology Data Exchange (ETDEWEB)

    Hart, W.; Istrail, S.; Atkins, J. [Sandia National Labs., Albuquerque, NM (United States)

    1997-12-31

    Understanding the structure and function of proteins is a fundamental endeavor in molecular biology. Currently, over 100,000 protein sequences have been determined by experimental methods. The three dimensional structure of the protein determines its function, but there are currently less than 4,000 structures known to atomic resolution. Accordingly, techniques to predict protein structure from sequence have an important role in aiding the understanding of the Genome and the effects of mutations in genetic disease. The authors describe current efforts at Sandia to better understand the structure of proteins through rigorous mathematical analyses of simple lattice models. The efforts have focused on two aspects of protein science: mathematical structure prediction, and inverse protein folding.

  11. The Papillomavirus E2 proteins

    Energy Technology Data Exchange (ETDEWEB)

    McBride, Alison A., E-mail: amcbride@nih.gov

    2013-10-15

    The papillomavirus E2 proteins are pivotal to the viral life cycle and have well characterized functions in transcriptional regulation, initiation of DNA replication and partitioning the viral genome. The E2 proteins also function in vegetative DNA replication, post-transcriptional processes and possibly packaging. This review describes structural and functional aspects of the E2 proteins and their binding sites on the viral genome. It is intended to be a reference guide to this viral protein. - Highlights: • Overview of E2 protein functions. • Structural domains of the papillomavirus E2 proteins. • Analysis of E2 binding sites in different genera of papillomaviruses. • Compilation of E2 associated proteins. • Comparison of key mutations in distinct E2 functions.

  12. Protein-protein interaction based on pairwise similarity

    Directory of Open Access Journals (Sweden)

    Zaki Nazar

    2009-05-01

    Full Text Available Abstract Background Protein-protein interaction (PPI is essential to most biological processes. Abnormal interactions may have implications in a number of neurological syndromes. Given that the association and dissociation of protein molecules is crucial, computational tools capable of effectively identifying PPI are desirable. In this paper, we propose a simple yet effective method to detect PPI based on pairwise similarity and using only the primary structure of the protein. The PPI based on Pairwise Similarity (PPI-PS method consists of a representation of each protein sequence by a vector of pairwise similarities against large subsequences of amino acids created by a shifting window which passes over concatenated protein training sequences. Each coordinate of this vector is typically the E-value of the Smith-Waterman score. These vectors are then used to compute the kernel matrix which will be exploited in conjunction with support vector machines. Results To assess the ability of the proposed method to recognize the difference between "interacted" and "non-interacted" proteins pairs, we applied it on different datasets from the available yeast saccharomyces cerevisiae protein interaction. The proposed method achieved reasonable improvement over the existing state-of-the-art methods for PPI prediction. Conclusion Pairwise similarity score provides a relevant measure of similarity between protein sequences. This similarity incorporates biological knowledge about proteins and it is extremely powerful when combined with support vector machine to predict PPI.

  13. A new protein structure representation for efficient protein function prediction.

    Science.gov (United States)

    Maghawry, Huda A; Mostafa, Mostafa G M; Gharib, Tarek F

    2014-12-01

    One of the challenging problems in bioinformatics is the prediction of protein function. Protein function is the main key that can be used to classify different proteins. Protein function can be inferred experimentally with very small throughput or computationally with very high throughput. Computational methods are sequence based or structure based. Structure-based methods produce more accurate protein function prediction. In this article, we propose a new protein structure representation for efficient protein function prediction. The representation is based on three-dimensional patterns of protein residues. In the analysis, we used protein function based on enzyme activity through six mechanistically diverse enzyme superfamilies: amidohydrolase, crotonase, haloacid dehalogenase, isoprenoid synthase type I, and vicinal oxygen chelate. We applied three different classification methods, naïve Bayes, k-nearest neighbors, and random forest, to predict the enzyme superfamily of a given protein. The prediction accuracy using the proposed representation outperforms a recently introduced representation method that is based only on the distance patterns. The results show that the proposed representation achieved prediction accuracy up to 98%, with improvement of about 10% on average. PMID:25343279

  14. Bioinformatic Prediction of WSSV-Host Protein-Protein Interaction

    Directory of Open Access Journals (Sweden)

    Zheng Sun

    2014-01-01

    Full Text Available WSSV is one of the most dangerous pathogens in shrimp aquaculture. However, the molecular mechanism of how WSSV interacts with shrimp is still not very clear. In the present study, bioinformatic approaches were used to predict interactions between proteins from WSSV and shrimp. The genome data of WSSV (NC_003225.1 and the constructed transcriptome data of F. chinensis were used to screen potentially interacting proteins by searching in protein interaction databases, including STRING, Reactome, and DIP. Forty-four pairs of proteins were suggested to have interactions between WSSV and the shrimp. Gene ontology analysis revealed that 6 pairs of these interacting proteins were classified into “extracellular region” or “receptor complex” GO-terms. KEGG pathway analysis showed that they were involved in the “ECM-receptor interaction pathway.” In the 6 pairs of interacting proteins, an envelope protein called “collagen-like protein” (WSSV-CLP encoded by an early virus gene “wsv001” in WSSV interacted with 6 deduced proteins from the shrimp, including three integrin alpha (ITGA, two integrin beta (ITGB, and one syndecan (SDC. Sequence analysis on WSSV-CLP, ITGA, ITGB, and SDC revealed that they possessed the sequence features for protein-protein interactions. This study might provide new insights into the interaction mechanisms between WSSV and shrimp.

  15. Peptides and proteins

    Energy Technology Data Exchange (ETDEWEB)

    Bachovchin, W.W.; Unkefer, C.J.

    1994-12-01

    Advances in magnetic resonance and vibrational spectroscopy make it possible to derive detailed structural information about biomolecular structures in solution. These techniques are critically dependent on the availability of labeled compounds. For example, NMR techniques used today to derive peptide and protein structures require uniformity {sup 13}C-and {sup 15}N-labeled samples that are derived biosynthetically from (U-6-{sup 13}C) glucose. These experiments are possible now because, during the 1970s, the National Stable Isotope Resource developed algal methods for producing (U-6-{sup 13}C) glucose. If NMR techniques are to be used to study larger proteins, we will need sophisticated labelling patterns in amino acids that employ a combination of {sup 2}H, {sup 13}C, and {sup 15}N labeling. The availability of these specifically labeled amino acids requires a renewed investment in new methods for chemical synthesis of labeled amino acids. The development of new magnetic resonance or vibrational techniques to elucidate biomolecular structure will be seriously impeded if we do not see rapid progress in labeling technology. Investment in labeling chemistry is as important as investment in the development of advanced spectroscopic tools.

  16. Protein Chemical Shift Prediction

    CERN Document Server

    Larsen, Anders S

    2014-01-01

    The protein chemical shifts holds a large amount of information about the 3-dimensional structure of the protein. A number of chemical shift predictors based on the relationship between structures resolved with X-ray crystallography and the corresponding experimental chemical shifts have been developed. These empirical predictors are very accurate on X-ray structures but tends to be insensitive to small structural changes. To overcome this limitation it has been suggested to make chemical shift predictors based on quantum mechanical(QM) calculations. In this thesis the development of the QM derived chemical shift predictor Procs14 is presented. Procs14 is based on 2.35 million density functional theory(DFT) calculations on tripeptides and contains corrections for hydrogen bonding, ring current and the effect of the previous and following residue. Procs14 is capable at performing predictions for the 13CA, 13CB, 13CO, 15NH, 1HN and 1HA backbone atoms. In order to benchmark Procs14, a number of QM NMR calculatio...

  17. Water-transporting proteins.

    Science.gov (United States)

    Zeuthen, Thomas

    2010-04-01

    Transport through lipids and aquaporins is osmotic and entirely driven by the difference in osmotic pressure. Water transport in cotransporters and uniporters is different: Water can be cotransported, energized by coupling to the substrate flux by a mechanism closely associated with protein. In the K(+)/Cl(-) and the Na(+)/K(+)/2Cl(-) cotransporters, water is entirely cotransported, while water transport in glucose uniporters and Na(+)-coupled transporters of nutrients and neurotransmitters takes place by both osmosis and cotransport. The molecular mechanism behind cotransport of water is not clear. It is associated with the substrate movements in aqueous pathways within the protein; a conventional unstirred layer mechanism can be ruled out, due to high rates of diffusion in the cytoplasm. The physiological roles of the various modes of water transport are reviewed in relation to epithelial transport. Epithelial water transport is energized by the movements of ions, but how the coupling takes place is uncertain. All epithelia can transport water uphill against an osmotic gradient, which is hard to explain by simple osmosis. Furthermore, genetic removal of aquaporins has not given support to osmosis as the exclusive mode of transport. Water cotransport can explain the coupling between ion and water transport, a major fraction of transepithelial water transport and uphill water transport. Aquaporins enhance water transport by utilizing osmotic gradients and cause the osmolarity of the transportate to approach isotonicity. PMID:20091162

  18. Bioactive proteins from pipefishes

    Institute of Scientific and Technical Information of China (English)

    E. Rethna Priya; S. Ravichandran; R. Ezhilmathi

    2013-01-01

    Objective: To screen antimicrobial potence of some pipefish species collected from Tuticorin coastal environment.Methods:Antimicrobial activity of pipefishes in methanol extract was investigated against 10 bacterial and 10 fungal human pathogenic strains.Results:Among the tested strains, in Centriscus scutatus, pipefish showed maximum zone of inhibition against Vibrio cholerae (8 mm) and minimum in the sample of Hippichthys cyanospilos against Klebseilla pneumoniae (2 mm). In positive control, maximum zone of inhibition was recorded in Vibrio cholerae (9 mm) and minimum in Klebseilla pneumoniae, and Salmonella paratyphi (5 mm). Chemical investigation indicated the presence of peptides as evidenced by ninhydrin positive spots on thin layer chromatography and presence of peptide. In SDS PAGE, in Centriscus scutatus, four bands were detected in the gel that represented the presence of proteins in the range nearly 25.8-75 kDa. In Hippichthys cyanospilos, five bands were detected in the gel that represented the presence of proteins in the range nearly 20.5-78 kDa. The result of FT-IR spectrum revealed that the pipe fishes extracts compriseed to have peptide derivatives as their predominant chemical groups.Conclusions:It can be conclude that this present investigation suggests the tested pipe fishes will be a potential source of natural bioactive compounds.

  19. Prion protein in milk.

    Directory of Open Access Journals (Sweden)

    Nicola Franscini

    Full Text Available BACKGROUND: Prions are known to cause transmissible spongiform encephalopathies (TSE after accumulation in the central nervous system. There is increasing evidence that prions are also present in body fluids and that prion infection by blood transmission is possible. The low concentration of the proteinaceous agent in body fluids and its long incubation time complicate epidemiologic analysis and estimation of spreading and thus the risk of human infection. This situation is particularly unsatisfactory for food and pharmaceutical industries, given the lack of sensitive tools for monitoring the infectious agent. METHODOLOGY/PRINCIPAL FINDINGS: We have developed an adsorption matrix, Alicon PrioTrap, which binds with high affinity and specificity to prion proteins. Thus we were able to identify prion protein (PrP(C--the precursor of prions (PrP(Sc--in milk from humans, cows, sheep, and goats. The absolute amount of PrP(C differs between the species (from microg/l range in sheep to ng/l range in human milk. PrP(C is also found in homogenised and pasteurised off-the-shelf milk, and even ultrahigh temperature treatment only partially diminishes endogenous PrP(C concentration. CONCLUSIONS/SIGNIFICANCE: In view of a recent study showing evidence of prion replication occurring in the mammary gland of scrapie infected sheep suffering from mastitis, the appearance of PrP(C in milk implies the possibility that milk of TSE-infected animals serves as source for PrP(Sc.

  20. Rat myocardial protein degradation.

    Science.gov (United States)

    Steer, J H; Hopkins, B E

    1981-07-01

    1. Myocardial protein degradation rates were determined by following tyrosine release from rat isolated left hemi-atria in vitro. 2. After two 20 min preincubations the rate of tyrosine release from hemi-atria was constant for 4 h. 3. Skeletal muscle protein degradation was determined by following tyrosine release from rat isolated hemi-diaphragm (Fulks, Li & Goldberg, 1975). 4. Insulin (10(-7) M) inhibited tyrosine release from hemi-atria and hemi-diaphragm to a similar extent. A 48 h fast increased tyrosine release rate from hemi-diaphragm and decreased tyrosine release rate from hemi-atria. Hemi-diaphragm tyrosine release was inhibited by 15 mmol/l D-glucose but a variety of concentrations of D-glucose (0, 5, 15 mmol/l) had no effect on tyrosine release from hemi-atria. Five times the normal plasma levels of the branched-chain amino acids leucine, isoleucine and valine had no effect on tyrosine release from either hemi-atria or hemi-diaphragm.

  1. THE CLINICAL EXPRESSION OF HEREDITARY PROTEIN-C AND PROTEIN-S DEFICIENCY - A RELATION TO CLINICAL THROMBOTIC RISK-FACTORS AND TO LEVELS OF PROTEIN-C AND PROTEIN-S

    NARCIS (Netherlands)

    HENKENS, CMA; VANDERMEER, J; HILLEGE, JL; BOM, VJJ; HALIE, MR; van der Schaaf, W

    1993-01-01

    We investigated 103 first-degree relatives of 13 unrelated protein C or protein S deficient patients to assess the role of additional thrombotic risk factors and of protein C and protein S levels in the clinical expression of hereditary protein C and protein S deficiency. Fifty-seven relatives were

  2. Laboratory and outside the laboratory measurements of ginned and ot ginned cotton for fiber micronaire and maturity by portable Near Infrared (NIR) Instruments

    Science.gov (United States)

    Micronaire is a key cotton quality assessment property, impacting downstream fiber processing and dye consistency. A component of micronaire is fiber maturity (degree of secondary wall development). Historically, micronaire and maturity are measured in a laboratory under tight environmental condit...

  3. Neurocognitive derivation of protein surface property from protein aggregate parameters

    Science.gov (United States)

    Mishra, Hrishikesh; Lahiri, Tapobrata

    2011-01-01

    Current work targeted to predicate parametric relationship between aggregate and individual property of a protein. In this approach, we considered individual property of a protein as its Surface Roughness Index (SRI) which was shown to have potential to classify SCOP protein families. The bulk property was however considered as Intensity Level based Multi-fractal Dimension (ILMFD) of ordinary microscopic images of heat denatured protein aggregates which was known to have potential to serve as protein marker. The protocol used multiple ILMFD inputs obtained for a protein to produce a set of mapped outputs as possible SRI candidates. The outputs were further clustered and largest cluster centre after normalization was found to be a close approximation of expected SRI that was calculated from known PDB structure. The outcome showed that faster derivation of individual protein’s surface property might be possible using its bulk form, heat denatured aggregates. PMID:21572883

  4. Computational Prediction of Protein-Protein Interactions of Human Tyrosinase

    Directory of Open Access Journals (Sweden)

    Su-Fang Wang

    2012-01-01

    Full Text Available The various studies on tyrosinase have recently gained the attention of researchers due to their potential application values and the biological functions. In this study, we predicted the 3D structure of human tyrosinase and simulated the protein-protein interactions between tyrosinase and three binding partners, four and half LIM domains 2 (FHL2, cytochrome b-245 alpha polypeptide (CYBA, and RNA-binding motif protein 9 (RBM9. Our interaction simulations showed significant binding energy scores of −595.3 kcal/mol for FHL2, −859.1 kcal/mol for CYBA, and −821.3 kcal/mol for RBM9. We also investigated the residues of each protein facing toward the predicted site of interaction with tyrosinase. Our computational predictions will be useful for elucidating the protein-protein interactions of tyrosinase and studying its binding mechanisms.

  5. Expanding coordination chemistry from protein to protein assembly.

    Science.gov (United States)

    Sanghamitra, Nusrat J M; Ueno, Takafumi

    2013-05-14

    Bioinorganic chemistry is of growing importance in the fields of nanomaterial science and biotechnology. Coordination of metals by biological systems is a crucial step in intricate enzymatic reactions such as photosynthesis, nitrogen fixation and biomineralization. Although such systems employ protein assemblies as molecular scaffolds, the important roles of protein assemblies in coordination chemistry have not been systematically investigated and characterized. Many researchers are joining the field of bioinorganic chemistry to investigate the inorganic chemistry of protein assemblies. This area is emerging as an important next-generation research field in bioinorganic chemistry. This article reviews recent progress in rational design of protein assemblies in coordination chemistry for integration of catalytic reactions using metal complexes, preparation of mineral biomimetics, and mechanistic investigations of biomineralization processes with protein assemblies. The unique chemical properties of protein assemblies in the form of cages, tubes, and crystals are described in this review.

  6. Mapping Protein-Protein Interactions by Quantitative Proteomics

    DEFF Research Database (Denmark)

    Dengjel, Joern; Kratchmarova, Irina; Blagoev, Blagoy

    2010-01-01

    Proteins exert their function inside a cell generally in multiprotein complexes. These complexes are highly dynamic structures changing their composition over time and cell state. The same protein may thereby fulfill different functions depending on its binding partners. Quantitative mass...... spectrometry (MS)-based proteomics in combination with affinity purification protocols has become the method of choice to map and track the dynamic changes in protein-protein interactions, including the ones occurring during cellular signaling events. Different quantitative MS strategies have been used to...... characterize protein interaction networks. In this chapter we describe in detail the use of stable isotope labeling by amino acids in cell culture (SILAC) for the quantitative analysis of stimulus-dependent dynamic protein interactions....

  7. Protein secretion in Pichia pastoris and advances in protein production.

    Science.gov (United States)

    Damasceno, Leonardo M; Huang, Chung-Jr; Batt, Carl A

    2012-01-01

    Yeast expression systems have been successfully used for over 20 years for the production of recombinant proteins. With the growing interest in recombinant protein expression for various uses, yeast expression systems, such as the popular Pichia pastoris, are becoming increasingly important. Although P. pastoris has been successfully used in the production of many secreted and intracellular recombinant proteins, there is still room for improvement of this expression system. In particular, secretion of recombinant proteins is still one of the main reasons for using P. pastoris. Therefore, endoplasmic reticulum protein folding, correct glycosylation, vesicular transport to the plasma membrane, gene dosage, secretion signal sequences, and secretome studies are important considerations for improved recombinant protein production. PMID:22057543

  8. Protein-water dynamics in antifreeze protein III activity

    Science.gov (United States)

    Xu, Yao; Bäumer, Alexander; Meister, Konrad; Bischak, Connor G.; DeVries, Arthur L.; Leitner, David M.; Havenith, Martina

    2016-03-01

    We combine Terahertz absorption spectroscopy (THz) and molecular dynamics (MD) simulations to investigate the underlying molecular mechanism for the antifreeze activity of one class of antifreeze protein, antifreeze protein type III (AFP-III) with a focus on the collective water hydrogen bond dynamics near the protein. After summarizing our previous work on AFPs, we present a new investigation of the effects of cosolutes on protein antifreeze activity by adding sodium citrate to the protein solution of AFP-III. Our results reveal that for AFP-III, unlike some other AFPs, the addition of the osmolyte sodium citrate does not affect the hydrogen bond dynamics at the protein surface significantly, as indicated by concentration dependent THz measurements. The present data, in combination with our previous THz measurements and molecular simulations, confirm that while long-range solvent perturbation is a necessary condition for the antifreeze activity of AFP-III, the local binding affinity determines the size of the hysteresis.

  9. Proteins interacting with cloning scars: a source of false positive protein-protein interactions.

    Science.gov (United States)

    Banks, Charles A S; Boanca, Gina; Lee, Zachary T; Florens, Laurence; Washburn, Michael P

    2015-01-01

    A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as putative interacting proteins using mass spectrometry. Control experiments can be used to limit false-positive results, but in many cases, there are still a surprising number of prey proteins that appear to copurify specifically with the bait. Here, we have identified one source of false-positive interactions in such studies. We have found that a combination of: 1) the variable sequence of the C-terminus of the bait with 2) a C-terminal valine "cloning scar" present in a commercially available ORF library, can in some cases create a peptide motif that results in the aberrant co-purification of endogenous cellular proteins. Control experiments may not identify false positives resulting from such artificial motifs, as aberrant binding depends on sequences that vary from one bait to another. It is possible that such cryptic protein binding might occur in other systems using affinity tagged proteins; this study highlights the importance of conducting careful follow-up studies where novel protein-protein interactions are suspected.

  10. A computational system for modelling flexible protein-protein and protein-DNA docking.

    Science.gov (United States)

    Sternberg, M J; Aloy, P; Gabb, H A; Jackson, R M; Moont, G; Querol, E; Aviles, F X

    1998-01-01

    A computational system is described that predicts the structure of protein/protein and protein/DNA complexes starting from unbound coordinate sets. The approach is (i) a global search with rigid-body docking for complexes with shape complementarity and favourable electrostatics; (ii) use of distance constraints from experimental (or predicted) knowledge of critical residues; (iii) use of pair potential to screen docked complexes and (iv) refinement and further screening by protein-side chain optimisation and interfacial energy minimisation. The system has been applied to model ten protein/protein and eight protein-repressor/DNA (steps i to iii only) complexes. In general a few complexes, one of which is close to the true structure, can be generated. PMID:9783224

  11. Multifunctional proteins revealed by overlapping clustering in protein interaction network

    OpenAIRE

    Becker, Emmanuelle; Robisson, Benoît; Chapple, Charles E.; Guénoche, Alain; Brun, Christine

    2011-01-01

    Motivation: Multifunctional proteins perform several functions. They are expected to interact specifically with distinct sets of partners, simultaneously or not, depending on the function performed. Current graph clustering methods usually allow a protein to belong to only one cluster, therefore impeding a realistic assignment of multifunctional proteins to clusters. Results: Here, we present Overlapping Cluster Generator (OCG), a novel clustering method which decomposes a network into overla...

  12. Vicinal solvent and protein coupling modulates the protein dynamics

    OpenAIRE

    Sezerman, Ayşe Özlem; Sezerman, Ayse Ozlem

    2007-01-01

    The dynamics of a folded protein is studied in water and glycerol at a series of temperatures below and above their respective dynamical transition. The system is modeled in two distinct states whereby the protein is decoupled from the bulk solvent at low temperatures, and communicates with it through a vicinal layer at physiological temperatures. A linear viscoelastic model elucidates the less-than-expected increase in the relaxation times observed in the backbone dynamics of the protein. Th...

  13. Protein Adaptations in Archaeal Extremophiles

    Directory of Open Access Journals (Sweden)

    Christopher J. Reed

    2013-01-01

    Full Text Available Extremophiles, especially those in Archaea, have a myriad of adaptations that keep their cellular proteins stable and active under the extreme conditions in which they live. Rather than having one basic set of adaptations that works for all environments, Archaea have evolved separate protein features that are customized for each environment. We categorized the Archaea into three general groups to describe what is known about their protein adaptations: thermophilic, psychrophilic, and halophilic. Thermophilic proteins tend to have a prominent hydrophobic core and increased electrostatic interactions to maintain activity at high temperatures. Psychrophilic proteins have a reduced hydrophobic core and a less charged protein surface to maintain flexibility and activity under cold temperatures. Halophilic proteins are characterized by increased negative surface charge due to increased acidic amino acid content and peptide insertions, which compensates for the extreme ionic conditions. While acidophiles, alkaliphiles, and piezophiles are their own class of Archaea, their protein adaptations toward pH and pressure are less discernible. By understanding the protein adaptations used by archaeal extremophiles, we hope to be able to engineer and utilize proteins for industrial, environmental, and biotechnological applications where function in extreme conditions is required for activity.

  14. Proteins aggregation and human diseases

    Science.gov (United States)

    Hu, Chin-Kun

    2015-04-01

    Many human diseases and the death of most supercentenarians are related to protein aggregation. Neurodegenerative diseases include Alzheimer's disease (AD), Huntington's disease (HD), Parkinson's disease (PD), frontotemporallobar degeneration, etc. Such diseases are due to progressive loss of structure or function of neurons caused by protein aggregation. For example, AD is considered to be related to aggregation of Aβ40 (peptide with 40 amino acids) and Aβ42 (peptide with 42 amino acids) and HD is considered to be related to aggregation of polyQ (polyglutamine) peptides. In this paper, we briefly review our recent discovery of key factors for protein aggregation. We used a lattice model to study the aggregation rates of proteins and found that the probability for a protein sequence to appear in the conformation of the aggregated state can be used to determine the temperature at which proteins can aggregate most quickly. We used molecular dynamics and simple models of polymer chains to study relaxation and aggregation of proteins under various conditions and found that when the bending-angle dependent and torsion-angle dependent interactions are zero or very small, then protein chains tend to aggregate at lower temperatures. All atom models were used to identify a key peptide chain for the aggregation of insulin chains and to find that two polyQ chains prefer anti-parallel conformation. It is pointed out that in many cases, protein aggregation does not result from protein mis-folding. A potential drug from Chinese medicine was found for Alzheimer's disease.

  15. Topology independent protein structural alignment

    Directory of Open Access Journals (Sweden)

    DasGupta Bhaskar

    2007-10-01

    Full Text Available Abstract Background Identifying structurally similar proteins with different chain topologies can aid studies in homology modeling, protein folding, protein design, and protein evolution. These include circular permuted protein structures, and the more general cases of non-cyclic permutations between similar structures, which are related by non-topological rearrangement beyond circular permutation. We present a method based on an approximation algorithm that finds sequence-order independent structural alignments that are close to optimal. We formulate the structural alignment problem as a special case of the maximum-weight independent set problem, and solve this computationally intensive problem approximately by iteratively solving relaxations of a corresponding integer programming problem. The resulting structural alignment is sequence order independent. Our method is also insensitive to insertions, deletions, and gaps. Results Using a novel similarity score and a statistical model for significance p-value, we are able to discover previously unknown circular permuted proteins between nucleoplasmin-core protein and auxin binding protein, between aspartate rasemase and 3-dehydrogenate dehydralase, as well as between migration inhibition factor and arginine repressor which involves an additional strand-swapping. We also report the finding of non-cyclic permuted protein structures existing in nature between AML1/core binding factor and ribofalvin synthase. Our method can be used for large scale alignment of protein structures regardless of the topology. Conclusion The approximation algorithm introduced in this work can find good solutions for the problem of protein structure alignment. Furthermore, this algorithm can detect topological differences between two spatially similar protein structures. The alignment between MIF and the arginine repressor demonstrates our algorithm's ability to detect structural similarities even when spatial

  16. Protein (Viridiplantae): 308800396 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available med protein product Ostreococcus tauri MTCVGNLDENMQVQDDDSRPARDKAKANFAQKAARKALERQEKDRLSARSASVLARFSSIRHLRISSYQLVPARTSWSILFTHTINTFGHSAGTYQYADTYQEIPDTHFKQSYQQSGQPRTGGWTFSSRAVIRVWLRGSP ...

  17. Protein (Cyanobacteria): 271116 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available protein Gloeocapsa sp. PCC 7428 MRDSAVEILLVEDNPCDAELTLHSLKSSNLTNHIEVVRDGAEALDFIFCTGNYAHRSMDDEPRVILLDLKLPKVDGLEVLQKIKSDPRTRIIPVVVLTSSREERDIVDSYQLGVNSYIVKPVDFEQFTEAVRQLGLYWRLLNQPPVS ...

  18. Protein (Cyanobacteria): 295749 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ceiver protein Nostoc sp. PCC 7107 MPIEVLLVEDNPGDAELTRIALQDSKISINLNIVEDGVEAMAFLRKQDSYTRKPHPDIVLLDLNLPRKDGREVLAEMKSDDHLKRIPVVVLTTSQSEEDILKAYNLAANCYITKPVDFDQFVKIVQSIENFWFAIVKLPPE ...

  19. Protein (Cyanobacteria): 330437 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available 27 protein Synechococcus sp. BL107 MLSALARLFKPLTKAAVALGLGLCLLLTACSGDPDARLTGNYADDTISVAQTILEVIDIPQDDPSHAQAENDARSLITDYVSRYRPQPRVNGLSSFTTMQTALNSLAGHYANYANRPLPEALHDRLAKELTKAQKAVVRGT ...

  20. Protein (Cyanobacteria): 351371 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available tical protein Oscillatoria sp. PCC 6506 MAGVFFAFSTFVMSALARLQPAQGISAMQAINITAINPLFMTALFGTAAACIFLAISSLLKWHQSAAYLLVGSLLYLVGTVGVTIAFNVPLNDALAIVTPDSTEGANLWARYLTDWTFWNHVRTIAALAASALLTIALCV ...

  1. Protein (Viridiplantae): 159472102 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available 4474 predicted protein, partial Chlamydomonas reinhardtii PPSPAPPSPEPGSPPPSPAPPSPQPPSPAPPSPEPGSPPPSPAPPSPKPPSPAPPSPEQPGSPPPSPPPPRPQPPSPAPPSPEPGSPPPSPAPPSPQPPSPAPPSPEPGSPPPSPAPTQP ...

  2. Protein (Viridiplantae): 15227263 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available 93 putative protein kinase Arabidopsis thaliana MKLVLEGVDSFETLRVVGTFNCIDPDYVGSKRVTKKADVYAFEVILMELITGRKANYETLSVDEQNLVMWLRPKIKISTFLNLVDGTIATDKETIKRIKKIAKLAEYCTSQEVESRPLRASRTKSGNEVTSED ...

  3. Protein (Viridiplantae): 159485290 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available predicted protein Chlamydomonas reinhardtii MADAGPASGAMAAGVAAAPAVAGETVVGARAGPSGSGGVAGVDMADAGPASGAMAAGVAAAAAV...AGETVVGARAGPSGSGGVAGVDMADAGPASGAMAAGVAAAAAVAGETVVGARAGPSGSGGVAGVDMADAGPAGGAMAAATVAMLGAAAVASAWLLTACSPEGSGPGPS

  4. Protein (Cyanobacteria): 12321 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available protein Synechococcus sp. RCC307 MQGRSPAIGATTGLDEAYRLCRQQGLRLSRQRRLVLEILWRSGEHLSARDIFDRLNADGRRIGHTSVYQNLESLHSNGVIECLEKAQGRLYGHRADPHSHLTCLESGRISDLDIELPADLVEAIEQRTGFSIESYSLNLQGRPLP ...

  5. Protein (Cyanobacteria): 187027 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available domain protein Stanieria cyanosphaera PCC 7437 MRSQFITSIVFISAFIVLTLAGVKPVKTQLKQNSLQGCTTVYSFPVGGNLISKESESQINVREEPTVSAKVSDFGNQGEPIYVTQVFENNADGYCWYKVSFQSGAKGWVRGDFVSIFLASLAEAPLCSL ...

  6. Protein (Cyanobacteria): 133042 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ing protein Oscillatoriales cyanobacterium JSC-12 MYLLDTDIFIDIQRGFTPALSWFASLQELPSVPGFVVMELIQDAQNKQQVRKALQLVAPLPVVWPTEIDCARALSDFTAYHLSHKVGLIDSLITACAVGQSFTLCTFNTKHYRVIPGLKMEQPYTR ...

  7. Protein (Viridiplantae): 308807108 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available unnamed protein product Ostreococcus tauri MAIRKVEDDDDDPRAWYELACEAFDAGDRETCARALDRCETLDGAWARDARFALRRAHCAAAANDDGDERTMRAIDDVFRALDASGNDMPSDVRAVMVIDACGLEREWARRGGGETRAETERARERAMETLRNAPSE ...

  8. Protein (Viridiplantae): 308812394 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available unnamed protein product Ostreococcus tauri MGRHHTEYERAPPTRRVNARTWDDITQSTTPGCPAPRNIPRRPHPRASVTPPSHVDASTPRPNARTSARARGRERERERERASARSGGRSRAWRCKRGRGRGGGDRDRAHLFHRRRRRANERAIEANVEARCRC ...

  9. Protein (Viridiplantae): 255083122 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available predicted protein Micromonas sp. RCC299 MYASPALHRAVAFPKATKPAEASKAGRVATRAAAPEDKPAAAARPTGRRSFSVATLAAVVAAASAPDAALAFGSGIPGYDINEKARDAQRKAINDELAEQRELARKEKERRRLLKEAEEAEECARNPESCPAPAES ...

  10. Protein (Viridiplantae): 308802105 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available unnamed protein product Ostreococcus tauri MNLYWYTPGLSDITIANSLFPLLCSVCLDCQLSKLPSTSTLAGSVVAQSGNPPRAPSSPAMGTVGVCGLSHARHDSHRQSLAFARSIVPLSVSRDPNDPSDAVDHPRRSLARVTRIKNHRHTHPYDSASLPRAIRS ...

  11. Protein (Cyanobacteria): 212014 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available aining protein Nostoc sp. PCC 7524 MYATRCVIPIIKSPKDYQVYRISPHDTNRLAIIFDSTNANTSLTCCVEIFDIGGQTPPNRHQWAVEMFFVLKGEGVAICDGKKVPIAAGDSLLVPPTGTHLIKNIGSTRLYTLTIMVPNEDFSELIRSGTPMELDAEDMAVLGRI ...

  12. Protein (Viridiplantae): 159470577 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available predicted protein, partial Chlamydomonas reinhardtii MDALANKEVVFDLDAAAFSSDDFRIFQFKVKRCPRARPHDWTQCPFAHPGEKAKRRDPRKYRYSGTACPEFRRNGCCRRGDACPFAHGVFECWLHPSRYRTQMCTDGSNCKRRVCFFAHTESE ...

  13. Protein (Viridiplantae): 308805078 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available named protein product Ostreococcus tauri MKCGPTYATVDDRVIIEPNLRSHFVVGRATREYERLVQAIPNCFVGSYAQLTEIVHFVSQHMNASFRERGLDVPPWRRPSALTSKWTLRAPGSSVPPSPRDHARRIRTSRLHETKIHSFNIRLRTKLRGAGVLTRSI ...

  14. Protein (Cyanobacteria): 301492 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available g protein Thermosynechococcus elongatus BP-1 MPTPTYPKASLIIRERGLPKREVLLSPDRQWTIGRQLDCSIRLTDAYVSRLHAVINAFLFRGQPLYFIRDAHSRNGTFLNGFPLQHSTLLHHEDVIGVGTTLLVFYYPDMFREISLDECPELTKGSTDSLPWRS ...

  15. Protein (Cyanobacteria): 132951 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available protein C Synechococcus elongatus PCC 6301 MFLLDTNACIQLLNRRHPQLLQHFRQQSPADIALCSIVKSELLYGARRSQNVEANLQLLDRFFAPLQSLPFTDRCAEEAGLIRADPAAQGKPIGPNDLLIAATARAFDTTLVTYNTREFVRITGLRVVDWELANPLLS ...

  16. Protein (Viridiplantae): 255074049 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available predicted protein Micromonas sp. RCC299 MAKAAKPTVRVNTTKRTIGETSRYAGARDASPSPHVARSRDSIHETDHRIAPPSFLHLRGVGRRAAAAGFLALILSTNAAPAEAETKRRAAVPEKKAFSPYSSGGSGFGKYKAPRAFLKDEKAEVLKAYGLETLPGQ ...

  17. Protein (Cyanobacteria): 107518 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ical protein Microcystis aeruginosa PCC 7941 MFATDKYLELLKQYPPRSIHNEEDLEMMQEVINRLLDKPQLTTEEREYLNVLGALIYEYEENQEPIPDIYGLELLKFILEERNLQKQDLLSIFESKSTLDDIFDGLQELTPIYIQKLANFLNISPDLFFPS ...

  18. Protein (Cyanobacteria): 54715 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available ical protein Arthrospira maxima CS-328 MKLAYRGVNYNHEFQPMDVVESEVCGKYRGQDCHFHYPRHIPVPQPHGNLKYRAVSYSVGEPRDQNTMMVAVATSDAKPEPPPATSGDTCAIAPEELAKVHSANLCRLLERRQKAARERGDQRLLSLLEAEAQHIAC ...

  19. Versatile protein tagging in cells with split fluorescent protein

    OpenAIRE

    Kamiyama, Daichi; Sekine, Sayaka; Barsi-Rhyne, Benjamin; Hu, Jeffrey; Chen, Baohui; Gilbert, Luke A.; Ishikawa, Hiroaki; Leonetti, Manuel D.; Marshall, Wallace F.; Jonathan S Weissman; Huang, Bo

    2016-01-01

    In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small size of these tags may reduce functional perturbation and enable signal amplification. To address their background issue, we adapt self-complementing split fluorescent proteins as epitope tags for live cell protein labelling. The two tags, GFP11 and sfCherry11 are derived from the eleventh β-strand of super-folder GFP and sfCherry, respec...

  20. Calcineurin homologous protein: a multifunctional Ca2+-binding protein family

    OpenAIRE

    Di Sole, Francesca; Vadnagara, Komal; MOE, ORSON W.; Babich, Victor

    2012-01-01

    The calcineurin homologous protein (CHP) belongs to an evolutionarily conserved Ca2+-binding protein subfamily. The CHP subfamily is composed of CHP1, CHP2, and CHP3, which in vertebrates share significant homology at the protein level with each other and between other Ca2+-binding proteins. The CHP structure consists of two globular domains containing from one to four EF-hand structural motifs (calcium-binding regions composed of two helixes, E and F, joined by a loop), the myristoylation, a...

  1. Protein expression strategies for identification of novel target proteins.

    Science.gov (United States)

    Schuster, M; Wasserbauer, E; Einhauer, A; Ortner, C; Jungbauer, A; Hammerschmid, F; Werner, G

    2000-04-01

    Identification of new target proteins is a novel paradigm in drug discovery. A major bottleneck of this strategy is the rapid and simultaneous expression of proteins from differential gene expression to identify eligible candidates. By searching for a generic system enabling high throughput expression analysis and purification of unknown cDNAs, we evaluated the YEpFLAG-1 yeast expression system. We have selected cDNAs encoding model proteins (eukaryotic initiation factor-5A [eIF-5A] and Homo sapiens differentiation-dependent protein-A4) and cDNA encoding an unknown protein (UP-1) for overexpression in Saccharomyces cerevisiae using fusions with a peptide that changes its conformation in the presence of Ca2+ ions, the FLAG tag (Eastman Kodak, Rochester, NY). The cDNAs encoding unknown proteins originating from a directionally cloned cDNA library were expressed in all three possible reading frames. The expressed proteins were detected by an antibody directed against the FLAG tag and/or by antibodies against the model proteins. The alpha-leader sequence, encoding a yeast mating pheromone, upstream of the gene fusion site facilitates secretion into the culture supernatant. EIF-5A could be highly overexpressed and was secreted into the culture supernatant. In contrast, the Homo sapiens differentiation-dependent protein-A4 as well as the protein UP-1, whose cDNA did not match to any known gene, could not be detected in the culture supernatant. The expression product of the correct frame remained in the cells, whereas the FLAG-tagged proteins secreted into the supernatant were short, out-of-frame products. The presence of transmembrane domains or patches of hydrophobic amino acids may preclude secretion of these proteins into the culture supernatant. Subsequently, isolation and purification of the various proteins was accomplished by affinity chromatography or affinity extraction using magnetizable beads coated with the anti-FLAG monoclonal antibody. The purity of

  2. Exploring the repeat protein universe through computational protein design.

    Science.gov (United States)

    Brunette, T J; Parmeggiani, Fabio; Huang, Po-Ssu; Bhabha, Gira; Ekiert, Damian C; Tsutakawa, Susan E; Hura, Greg L; Tainer, John A; Baker, David

    2015-12-24

    A central question in protein evolution is the extent to which naturally occurring proteins sample the space of folded structures accessible to the polypeptide chain. Repeat proteins composed of multiple tandem copies of a modular structure unit are widespread in nature and have critical roles in molecular recognition, signalling, and other essential biological processes. Naturally occurring repeat proteins have been re-engineered for molecular recognition and modular scaffolding applications. Here we use computational protein design to investigate the space of folded structures that can be generated by tandem repeating a simple helix-loop-helix-loop structural motif. Eighty-three designs with sequences unrelated to known repeat proteins were experimentally characterized. Of these, 53 are monomeric and stable at 95 °C, and 43 have solution X-ray scattering spectra consistent with the design models. Crystal structures of 15 designs spanning a broad range of curvatures are in close agreement with the design models with root mean square deviations ranging from 0.7 to 2.5 Å. Our results show that existing repeat proteins occupy only a small fraction of the possible repeat protein sequence and structure space and that it is possible to design novel repeat proteins with precisely specified geometries, opening up a wide array of new possibilities for biomolecular engineering.

  3. Membrane Protein Solubilization and Composition of Protein Detergent Complexes.

    Science.gov (United States)

    Duquesne, Katia; Prima, Valérie; Sturgis, James N

    2016-01-01

    Membrane proteins are typically expressed in heterologous systems with a view to in vitro characterization. A critical step in the preparation of membrane proteins after expression in any system is the solubilization of the protein in aqueous solution, typically using detergents and lipids, to obtain the protein in a form suitable for purification, structural or functional analysis. This process is particularly difficult as the objective is to prepare the protein in an unnatural environment, a protein detergent complex, separating it from its natural lipid partners while causing the minimum destabilization or modification of the structure. Although the process is difficult, and relatively hard to master, an increasing number of membrane proteins have been successfully isolated after expression in a wide variety of systems. In this chapter we give a general protocol for preparing protein detergent complexes that is aimed at guiding the reader through the different critical steps. In the second part of the chapter we illustrate how to analyze the composition of protein detergent complexes; this analysis is important as it has been found that compositional variation often causes irreproducible results. PMID:27485340

  4. Protein Polymers and Amyloids

    DEFF Research Database (Denmark)

    Risør, Michael Wulff

    2014-01-01

    that inhibits its target protease through a large conformational change but mutations compromise this function and cause premature structural collapse into hyperstable polymers. Understanding the conformational disorders at a molecular level is not only important for our general knowledge on protein folding...... of this mechanism were investigated through a series of interaction experiments. Despite a very buried location in the native structure, evidence here suggest that the C-terminal tail is labile under slightly destabilizing conditions, providing new detail to this matter. A small infectious polymer unit was also...... constructed and used to show how polymerogenic seeding and polymer propagation might happen inside the body. The locking of central structural elements during α1AT folding or in the native state represents a therapeutic strategy to prevent polymerization. Using Molecular Dynamics simulations, we identified...

  5. Protein microarrays for systems biology

    Institute of Scientific and Technical Information of China (English)

    Lina Yang; Shujuan Guo; Yang Li; Shumin Zhou; Shengce Tao

    2011-01-01

    Systems biology holds the key for understanding biological systems on a system level. It eventually holds the key for the treatment and cure of complex diseases such as cancer,diabetes, obesity, mental disorders, and many others. The '-omics' technologies, such as genomics, transcriptomics,proteomics, and metabonomics, are among the major driving forces of systems biology. Featured as highthroughput, miniaturized, and capable of parallel analysis,protein microarrays have already become an important technology platform for systems biology, In this review, we will focus on the system level or global analysis of biological systems using protein microarrays. Four major types of protein microarrays will be discussed: proteome microarrays, antibody microarrays, reverse-phase protein arrays,and lectin microarrays. We will also discuss the challenges and future directions of protein microarray technologies and their applications for systems biology. We strongly believe that protein microarrays will soon become an indispensable and invaluable tool for systems biology.

  6. Water at interface with proteins

    CERN Document Server

    Franzese, Giancarlo; Iskrov, Svilen

    2010-01-01

    Water is essential for the activity of proteins. However, the effect of the properties of water on the behavior of proteins is only partially understood. Recently, several experiments have investigated the relation between the dynamics of the hydration water and the dynamics of protein. These works have generated a large amount of data whose interpretation is debated. New experiments measure the dynamics of water at low temperature on the surface of proteins, finding a qualitative change (crossover) that might be related to the slowing down and stop of the protein's activity (protein glass transition), possibly relevant for the safe preservation of organic material at low temperature. To better understand the experimental data several scenarios have been discussed. Here, we review these experiments and discuss their interpretations in relation with the anomalous properties of water. We summarize the results for the thermodynamics and dynamics of supercooled water at an interface. We consider also the effect o...

  7. Vegetable proteins and milk puddings

    OpenAIRE

    Sousa, Isabel; M.C. NUNES; Batista, P; Raymundo, Anabela; Alves, M. M.

    2003-01-01

    In recent years, interest in animal free foods has increased tremendously due to factors like BSE crisis, rise of nutritionally dependent illnesses, like diabetes type II, cardiovascular and digestive diseases, along with ethic orientations of denying animal intakes of any kind. The use of proteins from leguminous seeds as an alternative to the animal proteins in dairy desserts was studied. Lupin, pea and soya protein isolates were used in combination with k- carrageenan, gellan a...

  8. Protein intrinsic disorder in plants

    OpenAIRE

    Florencio ePazos; Natalia ePietrosemoli; García-Martín, Juan A.; Roberto eSolano

    2013-01-01

    To some extent contradicting the classical paradigm of the relationship between protein 3D structure and function, now it is clear that large portions of the proteomes, especially in higher organisms, lack a fixed structure and still perform very important functions. Proteins completely or partially unstructured in their native (functional) form are involved in key cellular processes underlain by complex networks of protein interactions. The intrinsic conformational flexibility of these disor...

  9. The Papillomavirus E2 Proteins

    OpenAIRE

    Alison A McBride

    2013-01-01

    The papillomavirus E2 proteins are pivotal to the viral life cycle and have well characterized functions in transcriptional regulation, initiation of DNA replication and partitioning the viral genome. The E2 proteins also function in vegetative DNA replication, post-transcriptional processes and possibly packaging. This review describes structural and functional aspects of the E2 proteins and their binding sites on the viral genome. It is intended to be a reference guide to ...

  10. Hydrophobic organization of membrane proteins

    OpenAIRE

    Rees, D C; DeAntonio, L.; Eisenberg, D.

    1989-01-01

    Membrane-exposed residues are more hydrophobic than buried interior residues in the transmembrane regions of the photosynthetic reaction center from Rhodobacter sphaeroides. This hydrophobic organization is opposite to that of water-soluble proteins. The relative polarities of interior and surface residues of membrane and water soluble proteins are not simply reversed, however. The hydrophobicities of interior residues of both membrane and water-soluble proteins are comparable, whereas the bi...

  11. [Protein toxins of Staphylococcus aureus].

    Science.gov (United States)

    Shamsutdinov, A F; Tiurin, Iu A

    2014-01-01

    Main scientific-research studies regarding protein bacterial toxins of the most widespread bacteria that belong to Staphylococcus spp. genus and in particular the most pathogenic species for humans--Staphylococcus aureus, are analyzed. Structural and biological properties of protein toxins that have received the name of staphylococcus pyrogenic toxins (PTSAg) are presented. Data regarding genetic regulation of secretion and synthesis of these toxins and 3 main regulatory genetic systems (agr--accessory gene regulator, xpr--extracellular protein regulator, sar--staphylococcal accessory regulator) that coordinate synthesis of the most important protein toxins and enzymes for virulence of S. aureus, are presented.

  12. Protein leverage and energy intake.

    Science.gov (United States)

    Gosby, A K; Conigrave, A D; Raubenheimer, D; Simpson, S J

    2014-03-01

    Increased energy intakes are contributing to overweight and obesity. Growing evidence supports the role of protein appetite in driving excess intake when dietary protein is diluted (the protein leverage hypothesis). Understanding the interactions between dietary macronutrient balance and nutrient-specific appetite systems will be required for designing dietary interventions that work with, rather than against, basic regulatory physiology. Data were collected from 38 published experimental trials measuring ad libitum intake in subjects confined to menus differing in macronutrient composition. Collectively, these trials encompassed considerable variation in percent protein (spanning 8-54% of total energy), carbohydrate (1.6-72%) and fat (11-66%). The data provide an opportunity to describe the individual and interactive effects of dietary protein, carbohydrate and fat on the control of total energy intake. Percent dietary protein was negatively associated with total energy intake (F = 6.9, P protein. The analysis strongly supports a role for protein leverage in lean, overweight and obese humans. A better appreciation of the targets and regulatory priorities for protein, carbohydrate and fat intake will inform the design of effective and health-promoting weight loss diets, food labelling policies, food production systems and regulatory frameworks.

  13. Update on protein structure prediction

    DEFF Research Database (Denmark)

    Hubbard, T; Tramontano, A; Barton, G;

    1996-01-01

    Computational tools for protein structure prediction are of great interest to molecular, structural and theoretical biologists due to a rapidly increasing number of protein sequences with no known structure. In October 1995, a workshop was held at IRBM to predict as much as possible about a number...... of proteins of biological interest using ab initio pre!diction of fold recognition methods. 112 protein sequences were collected via an open invitation for target submissions. 17 were selected for prediction during the workshop and for 11 of these a prediction of some reliability could be made. We believe...

  14. Molecular dynamics of membrane proteins.

    Energy Technology Data Exchange (ETDEWEB)

    Woolf, Thomas B. (Johns Hopkins University School of Medicine, Baltimore, MD); Crozier, Paul Stewart; Stevens, Mark Jackson

    2004-10-01

    Understanding the dynamics of the membrane protein rhodopsin will have broad implications for other membrane proteins and cellular signaling processes. Rhodopsin (Rho) is a light activated G-protein coupled receptor (GPCR). When activated by ligands, GPCRs bind and activate G-proteins residing within the cell and begin a signaling cascade that results in the cell's response to external stimuli. More than 50% of all current drugs are targeted toward G-proteins. Rho is the prototypical member of the class A GPCR superfamily. Understanding the activation of Rho and its interaction with its Gprotein can therefore lead to a wider understanding of the mechanisms of GPCR activation and G-protein activation. Understanding the dark to light transition of Rho is fully analogous to the general ligand binding and activation problem for GPCRs. This transition is dependent on the lipid environment. The effect of lipids on membrane protein activity in general has had little attention, but evidence is beginning to show a significant role for lipids in membrane protein activity. Using the LAMMPS program and simulation methods benchmarked under the IBIG program, we perform a variety of allatom molecular dynamics simulations of membrane proteins.

  15. Bromodomain Proteins in HIV Infection

    Directory of Open Access Journals (Sweden)

    Melanie Ott

    2013-06-01

    Full Text Available Bromodomains are conserved protein modules of ~110 amino acids that bind acetylated lysine residues in histone and non-histone proteins. Bromodomains are present in many chromatin-associated transcriptional regulators and have been linked to diverse aspects of the HIV life cycle, including transcription and integration. Here, we review the role of bromodomain-containing proteins in HIV infection. We begin with a focus on acetylated viral factors, followed by a discussion of structural and biological studies defining the involvement of bromodomain proteins in the HIV life cycle. We end with an overview of promising new studies of bromodomain inhibitory compounds for the treatment of HIV latency.

  16. Protein intrinsic disorder in plants

    Directory of Open Access Journals (Sweden)

    Florencio ePazos

    2013-09-01

    Full Text Available To some extent contradicting the classical paradigm of the relationship between protein 3D structure and function, now it is clear that large portions of the proteomes, especially in higher organisms, lack a fixed structure and still perform very important functions. Proteins completely or partially unstructured in their native (functional form are involved in key cellular processes underlain by complex networks of protein interactions. The intrinsic conformational flexibility of these disordered proteins allows them to bind multiple partners in transient interactions of high specificity and low affinity. In concordance, in plants this type of proteins has been found in processes requiring these complex and versatile interaction networks. These include transcription factor networks, where disordered proteins act as integrators of different signals or link different transcription factor subnetworks due to their ability to interact (in many cases simultaneously with different partners. Similarly, they also serve as signal integrators in signalling cascades, such as those related to response to external stimuli. Disordered proteins have also been found in plants in many stress-response processes, acting as protein chaperones or protecting other cellular components and structures. In plants, it is especially important to have complex and versatile networks able to quickly and efficiently respond to changing environmental conditions since these organisms can not escape and have no other choice than adapting to them. Consequently, protein disorder can play an especially important role in plants, providing them with a fast mechanism to obtain complex, interconnected and versatile molecular networks.

  17. High throughput protein production screening

    Science.gov (United States)

    Beernink, Peter T.; Coleman, Matthew A.; Segelke, Brent W.

    2009-09-08

    Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

  18. Protein stability: a crystallographer’s perspective

    Energy Technology Data Exchange (ETDEWEB)

    Deller, Marc C., E-mail: mdeller@stanford.edu [Stanford University, Shriram Center, 443 Via Ortega, Room 097, MC5082, Stanford, CA 94305-4125 (United States); Kong, Leopold [National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Building 8, Room 1A03, 8 Center Drive, Bethesda, MD 20814 (United States); Rupp, Bernhard [k.-k. Hofkristallamt, 91 Audrey Place, Vista, CA 92084 (United States); Medical University of Innsbruck, Schöpfstrasse 41, A-6020 Innsbruck (Austria)

    2016-01-26

    An understanding of protein stability is essential for optimizing the expression, purification and crystallization of proteins. In this review, discussion will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer. Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.

  19. Predicting disease genes using protein-protein interactions

    NARCIS (Netherlands)

    Oti, M.O.; Snel, B.; Huynen, M.A.; Brunner, H.G.

    2006-01-01

    BACKGROUND: The responsible genes have not yet been identified for many genetically mapped disease loci. Physically interacting proteins tend to be involved in the same cellular process, and mutations in their genes may lead to similar disease phenotypes. OBJECTIVE: To investigate whether protein-pr

  20. Protein linguistics - a grammar for modular protein assembly?

    Science.gov (United States)

    Gimona, Mario

    2006-01-01

    The correspondence between biology and linguistics at the level of sequence and lexical inventories, and of structure and syntax, has fuelled attempts to describe genome structure by the rules of formal linguistics. But how can we define protein linguistic rules? And how could compositional semantics improve our understanding of protein organization and functional plasticity?