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Sample records for arachis hypogaea

  1. Gamma irradiation of peanut (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    This study was conducted to determine some effects of gamma radiation on peanut (Arachis hypogaea L.). The biological parameters used to evaluate these effects were: % emergence of irradiated seeds, % survival of plants, growth rate, chlorophyll mutations, morphological changes and yield potential. Seeds were irradiated with dose levels of 2.5, 5, 10, 15, 20, 25, 30 and 40 KK per hour of gamma radiation from a Co-60 source. In general, % of emergence and survival in the M1 gene ration decreased with increasing doses of gamma radiation. Morphological changes induced by gamma radiation in the M1 generation of peanut were: leaf flecks, thickened leaves, red purple seeds and red colored seed coat. Generally, frequency of these morphological changes increased with increasing doses of gamma radiation. Protein and fat contents were increased by 2.8% to 1.5% respectively, while starch content decreased with 2.6% in the M2. On the basis of the results obtained, gamma radiation is an effective mutagenic agent in inducing various morphological and genetic changes in peanut

  2. Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea

    Science.gov (United States)

    Fávero, Alessandra Pereira; dos Santos, Rodrigo Furtado; Simpson, Charles E.; Valls, José Francisco Montenegro; Vello, Natal Antonio

    2015-01-01

    Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species, and one A-genome species as female parents. Of the 26 distinct cross combinations, 13 different interspecific AB-genome and three AA-genome hybrids were obtained. These sterile hybrids were polyploidized and five combinations produced tetraploid flowers. Next, 16 combinations were crossed between A. hypogaea and the synthetic amphidiploids, resulting in 11 different hybrid combinations. Our results confirm that it is possible to introgress resistance genes from wild species into the peanut using artificial hybridization, and that more species than previously reported can be used, thus enhancing the genetic variability in peanut genetic improvement programs. PMID:26500440

  3. Crystal structure of peanut (Arachis hypogaea) allergen Ara h 5

    Science.gov (United States)

    Profilins from numerous species are known to be allergens, including food allergens, such as peanut (Arachis hypogaea) allergen Ara h 5, and pollen allergens, such as birch allergen Bet v 2. Patients with pollen allergy can also cross-react to peanut. Structural characterization of allergens will al...

  4. Nutritional composition of new Peanut (Arachis hypogaea L. cultivars

    Directory of Open Access Journals (Sweden)

    2009-06-01

    Full Text Available Six peanut (Arachis hypogaea L. cultivars (Col-24-Gro, Col-61-Gto, VA-81-B, Ranferi Díaz, NC-2 and Florunner were studied for agricultural yield, chemical composition (protein, fat, carbohydrates, fiber and ash, amino acid profile, digestibility, fatty acid profile, tocopherol and sterol contents. Results indicated that Ranferi Díaz and Col-61-Gto presented the highest yield (6.3 Ton/ha. Protein content was from 23.5 to 26.6% and fat content ranged from 49.8-53.4%. Mean digestibility was 86%. Lysine and threonine levels in all cultivars were sufficient to meet human requirements. Total saturated fatty acids ranged from 15-18%. The oleic/linoleic ratio was estimated 1.3-1.4. Tocopherol levels varied from 390 to 706 ppm. The highest tocopherol levels corresponded to the cultivars with the lowest yield. The alpha tocopherol content was estimated at 90-150 ppm, while gamma tocopherol was 270-570 ppm.The main sterol present was βsitosterol (approx. 65%. Ranferi Diaz variety presented the highest agronomic yield and the highest protein content but low oleic acid, low sterols and low total tocopherols. The differences among cultivars suggest differences in their applications.

    Se estudio el rendimiento agrícola y composición química (proteína, grasa, carbohidratos, fibra y cenizas, perfil de amino ácidos, digestibilidad, perfil de ácidos grasos, contenido de tocoferol y de esteroles de seis variedades de cacahuate (Arachis hypogaea L. Col-24-Gro, Col-61-Gto, VA-81B, Ranferi Díaz, NC-2 y Florunner. Los resultados mostraron que el mayor rendimiento se logró en las variedades Ranferi Díaz y Col-61-Gto (6.3 Ton/ha. El contenido de proteína fue de 23.5 a 26.6% y el contenido de grasa en un intervalo de 49.8 a 53.4%. La digestibilidad promedio de las seis variedades fue de 86%. El contenido de lisina y treonina en la proteína de todas las variedades fue suficiente para satisfacer los requerimientos del humano. La composición del aceite

  5. Survey of Aspergillus and Aflatoxin in Groundnuts (Arachis hypogaea L.) and Groundnut Cake in Eastern Ethiopia

    Science.gov (United States)

    Groundnut (Arachis hypogaea L.) is an important cash and food crop in eastern Ethiopia. The lack of awareness and data on Aspergillus and aflatoxin contamination of groundnut and groundnut food products in the area are lacking. Therefore, this study was conducted to: i) assess major Aspergillus spec...

  6. LAS PROTEINAS SEMINALES DEL MANI (ARACHIS HYPOGAEA, LEGUMINOSAE y SU RELACION CON LAS CATEGORIAS INFRAESPECIFICAS

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    N R Grosso

    2010-11-01

    Full Text Available

    Las proteínas seminales de 122 muestras diferentes de Arachis hypogaea L. originarios de Bolivia, Perú y Ecuador fueron estudiadas por electroforesis en gel de poliacrilamida.
    Se detectaron siete bandas constantes y 27 bandas inconstantes. Los resultados de las últimas se utilizaron para analizar las similitudes entre las muestras empleando el coeficiente de Jaccard y el método de ligamiento promedio de la media aritmética no ponderada(UPGMA.
    Las proteínas seminales permitieron separar totalmente la subespecies de A.hypogaea y las variedades en menor medida.

  7. El cacahuate (Arachis hypogaea L.); como factor de desarrollo rural regional

    OpenAIRE

    Calixto Escobar, Elizabeth

    2012-01-01

    Esta investigación, se realizó en los Municipios de Ahuatlan, Tlapanala y Huaquechula, Puebla, México, con el objetivo de determinar los factores de una estrategia de desarrollo rural, orientado a los productores regionales de cacahuate (Arachis hypogaea L.), que fomente la incorporación de valor agregado al cultivo del cacahuate mediante su industrialización y comercialización. La metodología usada se desarrollo en dos fases; primera fase “Diagnóstico”: consistió en la recolección de la...

  8. Acclimation of peanut (Arachis hypogaea L.) to water stress through exposure to differing periods of early season drought

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) is able to withstand periods of water scarcity either in the early or late periods of the growing season, but suffers significant stress and yield loss during drought periods in mid-season, or the period coinciding with peak flower production and pod maturation. In fact...

  9. Size-selective fractionation and visual mapping of allergen protein chemistry in Arachis hypogaea.

    Science.gov (United States)

    Hebling, Christine M; Ross, Mark M; Callahan, John H; McFarland, Melinda A

    2012-11-01

    Peanuts (Arachis hypogaea) in addition to milk, eggs, fish, crustaceans, wheat, tree nuts, and soybean are commonly referred to as the "big eight" foods that contribute to the majority of food allergies worldwide. Despite the severity of allergic reactions and growing prevalence in children and adults, there is no cure for peanut allergy, leaving avoidance as the primary mode of treatment. To improve analytical methods for peanut allergen detection, researchers must overcome obstacles involved in handling complex food matrices while attempting to decipher the chemistry that underlies allergen protein interactions. To address such challenges, we conducted a global proteome characterization of raw peanuts using a sophisticated GELFrEE-PAGE-LC-MS/MS platform consisting of gel-based protein fractionation followed by mass spectrometric identification. The in-solution mass-selective protein fractionation: (1) enhances the number of unique peptide identifications, (2) provides a visual map of protein isoforms, and (3) aids in the identification of disulfide-linked protein complexes. GELFrEE-PAGE-LC-MS/MS not only overcomes many of the challenges involved in the study of plant proteomics, but enriches the understanding of peanut protein chemistry, which is typically unattainable in a traditional bottom-up proteomic analysis. A global understanding of protein chemistry in Arachis hypogaea ultimately will aid the development of improved methods for allergen detection in food. PMID:23020697

  10. Biomass Industrial Effluent Effect on Carbohydrates, Aminoacids, Nitrite and Nitrite Enzyme Activities of Arachis hypogaea L

    Institute of Scientific and Technical Information of China (English)

    PC Nagajyoti; N Dinakarc; S Suresh; Y Udaykiran; C Suresh; T N V K V Prasad; T Damodharam

    2009-01-01

    The objectives of the present investigations are to see the effects of biomass power plant effluent on the carbohydrates, aminoacids, nitrite and nitrite enzyme activitiesand proline of Arachis hypogaea L. vat TCGS 320 under controlled pot culture methods. Plants were cultivated with 25, 50, 75 and 100% of the effluent and a control without the effluent. The treatment of the crop with 25% of the effluent has shown stimulatory effect on all the biochemical parameters studied. Carbohydrates, starch, aminoacids, protein, nitrate and nitrite reductase enzymatic activities have increased in 10, 15, 20 DAS (days after sowing). In 25 and 30 DAS all biochemical parameters have decreased, due to environmental factors (rainfall, temperature, humidity, etc.). The present investigation clearly indicated that the biomass power plant effluent has stimulatory effect on all the biochemical contents at lower concentration, and at higher concentration they have deleterious effects.

  11. Effect of gamma irradiation on the grain yield of Nigerian Zea mays and Arachis hypogaea

    International Nuclear Information System (INIS)

    As a follow-up to our earlier investigation on the effect of gamma radiation on the germination and growth of certain Nigerian agricultural crops, the present study sought to determine the effect of gamma radiation on the grain yield of Zea mays (maize) and Arachis hypogaea (groundnut). The seeds were planted after irradiation without the application of fertiliser. The results show that for maize, grain yield for irradiated samples is increased to levels above the unirradiated yield at doses up to about 250 Gy with the optimum yield occurring at 150 Gy. The corresponding increase for groundnut is observed at doses up to about 930 Gy with optimum yield at a dose of 300 Gy. Inhibition in yield was observed to set in at a dose greater than 250 Gy for maize and 930 Gy for groundnut. The actual relationship between mean yield of these crops and gamma radiation dose was obtained using sixth-degree polynomial equations. (note)

  12. Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogaea L. seedlings

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Phytotoxicity of cadmium on growing Arachis hypogaea L. seedlings was studied. Seeds were exposed to 25, 50, and 100 μmol/L CdCl2 concentrations, for a period of 10, 15, 20 and 25 d. The extent of damage to chlorophyll, protein, proline, nitrate and nitrite reductase, antioxidant enzyme activity in leaves and roots were evaluated after 10 d of cadmium stress. The higher concentration of cadmium (100 μmol/L) resulted (leaves and roots) total chlorophyll 91.01%, protein 79.51%, 83.61%, nitrate reductase 79.39%,80.72% and nitrite reductase 77.07%, 75.88% activity decreased with increase in cadmium concentrations and exposure periods. Cadmium caused significant changes in the activity of antioxidative enzymes. Contrastingly Cd treated plant tissues showed an increase in proline 159.87%, 239.6%, gluthion reductase (GR) 337.72%, 306.14%, superoxide disumutase (SOD) 688.56%, 381.72%, ascorbate peroxidase (APX) 226.47%, 252.14%, peroxidase (POD) 72.19%, 60.29% and catalase (CAT) 228.96%, 214.74% as compared to control. Cadmium stress caused a significant increase in the rate of SOD activity in leaves and roots of plant species. Results show the crop A. hypogaea is highly sensitive even at very low cadmium concentrations.

  13. Genetic and functional diversities of bacterial communities in the rhizosphere of Arachis hypogaea.

    Science.gov (United States)

    Haldar, Shyamalina; Choudhury, Susanta Roy; Sengupta, Sanghamitra

    2011-06-01

    Bioinoculants are environmentally friendly, energy efficient and economically viable resources in sustainable agriculture. Knowledge of the structure and activities of microbial population in the rhizosphere of a plant is essential to formulate an effective bioinoculant. In this study, the bacterial community present in the rhizosphere of an important oilseed legume, Arachis hypogaea (L.) was described with respect to adjoining bulk soil as a baseline control using a 16S rDNA based metagenomic approach. Significantly higher abundance of Gamma-proteobacteria, a prevalence of Bacillus and the Cytophaga-Flavobacteria group of Bacteroidetes and absence of the Rhizobiaceae family of Alpha-proteobacteria were the major features observed in the matured Arachis-rhizosphere. The functional characterization of the rhizosphere-competent bacteria was performed using culture-dependent determination of phenotypes. Most bacterial isolates from the groundnut-rhizosphere exhibited multiple biochemical activities associated with plant growth and disease control. Validation of the beneficial traits in candidate bioinoculants in pot-cultures and field trials is necessary before their targeted application in the groundnut production system. PMID:21380504

  14. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L.) genome

    OpenAIRE

    Li Shaoxiong; Zhou Guiyuan; Liu Haiyan; Liang Xuanqiang; Chen Xiaoping; Hong Yanbin; Wen Shijie; Holbrook C Corley; Guo Baozhu

    2010-01-01

    Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L.) has and continues to be an important research goal to facilitate quantitative trait locus (QTL) analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanu...

  15. Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition ofArachis hypogaea

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    Mu Tan

    2016-01-01

    Full Text Available ABSTRACT The purpose of this research was to enhance the production of vitamin K2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37oC with initial pH value 7.0 ± 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM, optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K2HPO4 4.5 g/l,NaCl 3.0 g/l and MgSO4·7H2O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l. 3 mg/mL of arachis hypogaea was added into the medium at 72 h of shake-flake cultivation, which improved the production of menaquinone-4 (MK4 up to 371% and menaquinone-6 (MK6 up to 149% higher than those of the original medium. D-(+-catechin, one of the components of arachis hypogaea, was added alone into the medium, which also improved the vitamin K2 synthesis.

  16. Chemical composition of aboriginal peanut (Arachis hypogaea L. seeds from Uruguay.

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    Grosso, Nelson R.

    1999-06-01

    Full Text Available Oil, protein, carbohydrate and ash contents, iodine value, and fatty acid and sterol compositions were studied in seed of 9 aboriginal (Arachis hypogaea subsp. fastigiata var. vulgaris cultivars originating from Uruguay. They showed a high protein level as other varieties of the subspecie fastigiata (these protein percentages are higher than varieties of subspecie (hypogaea . Palmitic (16:0, stearic (18:0, oleic (18:1, linoleic (18:2, arachidic (20:0, eicosenoic (20:1, behenic (22:0, and lignoceric (24:0 acids were detected. The cultivar 7 Uv showed higher oleic acid content (42.53% and 0/L ratio (1.09. The cultivars 2 Uv and 5 Uv had higher percentage in linoleic acid (43.67% and 43.40%, respectively. The cultivar 3 Uv and 4 Uv exhibited lower iodine value (104.90 and 104.73, respectively. Cholesterol, campesterol, stigmasterol, β-sitosterol, Δ5-avenasterol, Δ7-estigmasteroI and Δ7-avenasterol were detected in the sterols, β-sitosterol was the most abundant. Significant difference was only found for stigmasterol. The chemical quality of these seeds is characterized by higher protein levels and oils with lower O/L ratio and higher iodine value.

    Se han estudiado los contenidos en aceite, proteína, hidrato de carbono y ceniza, índice de yodo y composición en ácidos grasos y esteroles en semillas de 9 cultivares aborígenes (Arachis hypogaea subsp. fastigiata var. vulgaris originarios de Uruguay. Estos mostraron un nivel alto en proteína como las otras variedades de la subespecie fastigiata (estos porcentajes en proteína son mayores que en las variedades de la subespecie (hypogaea . Se detectaron los ácidos palmítico (16:0, esteárico (18:0, oleico (18:1, linoleico (18:2, araquídico (20:0, eicosenoico (20:1, behénico (22:0 y lignocérico (24:0. El cultivar 7 Uv mostró el mayor contenido en ácido oleico (42. 53% y en la relación oleico

  17. Effect of Peanut (Arachis Hypogaea L. on Dyslipidemia in Young Adult

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    Hasina Akter,

    2015-06-01

    Full Text Available Background: Dyslipidemia is a major risk factor for cardiovascular disease. Lipid lowering drugs are available in modern medicine but prolong use of these drugs may produce some side effects. Peanut due to some of its active component can improve lipid profile. Objective: To observe the effects of peanut (Arachis hypogaea L. on dyslipidemia in young adults. Methods: This prospective interventional study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC, Dhaka between 1st July 2013 and 30th June 2014. For this purpose 30 dyslipidemic young adults of both sexes with aged 30 to 40 years were included in this study and they were selected from Out Patient Department of Medicine of Sir Salimullah Medical College and Mitford Hospital, Dhaka. They were studied three times i,e before supplementation with peanut (BSP, after 4 weeks supplementation with peanut (4 wks AP and after 8 weeks supplementation with peanut (8 wks AP. For assessing lipid profile TAG, LDL-C, TC and HDL-C of all subjects were estimated by enzymatic method. The statistical analysis was done by using paired sample‘t’ test as applicable. Results: In this study, the mean serum TAG, LDL-C and TC levels were significantly decreased after 8 weeks supplementation with peanut in comparison to those of after 4 weeks and before supplementation with peanut. However, these levels were also decreased after 4 weeks supplementation than those of before supplementation but it was significant only for TC (p<0.05 and LDL-C (p<0.001. Whereas, the mean serum HDL-C level was significantly (p<0.001 increased after 8 weeks supplementation when compared to those of after 4 weeks and before supplementation of peanut. Conclusion: The present study revealed that, peanut (Arachis hypogaea L. has effect on improvement of lipid profile by lowering serum TAG, LDL-C, TC levels and by increasing serum HDL-C level. This lipid lowering effects of peanut may be due to its high Mg

  18. Gamma radiation effects at color, antioxidant capacity and fatty acid profile in peanut (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    Irradiation is efficient at extinction fungi contamination in peanuts. Peanuts have high biologic value protein, minerals, vitamin E, complex B, and high concentration of lipids. The objective of this research is to evaluate the gamma irradiation effect on color, total phenolic, antioxidant activity, and fatty acid profile in peanuts (Arachis hypogaea L.). Cultivars IAC-Tatu ST and IAC-Runner 886 were submitted to gamma radiation with doses of 5.0; 7.5; 10.0, and 15.0 kGy and storage at room temperature. There was no significant difference in the color of IAC-Tatu ST. However, significant difference was found in the luminosity and Chroma in IAC-Runner 886. Total fenolics differed from the control with 33.27 mg.g-1 and treatment dose of 10.0 kGy with 58.60 mg.g-1 in IAC-Tatu ST. This parameter not had significant difference in IAC-Runner 886 and the control with 51.59 mg.g-1. The antioxidant activity did not present significant difference with a dose of 10.0 kGy, recommended for the elimination of fungi in peanuts. The dose of 10.0 kGy showed a decrease in saturated fatty acids, increase in unsaturated fatty acids, and an increase in linolleic acid. The oleic/linoleic relation decreased justifying further research correlating storage and oxidative stability. (author)

  19. Iron Oxide Nanoparticles as a Potential Iron Fertilizer for Peanut (Arachis hypogaea)

    Science.gov (United States)

    Rui, Mengmeng; Ma, Chuanxin; Hao, Yi; Guo, Jing; Rui, Yukui; Tang, Xinlian; Zhao, Qi; Fan, Xing; Zhang, Zetian; Hou, Tianqi; Zhu, Siyuan

    2016-01-01

    Nanomaterials are used in practically every aspect of modern life, including agriculture. The aim of this study was to evaluate the effectiveness of iron oxide nanoparticles (Fe2O3 NPs) as a fertilizer to replace traditional Fe fertilizers, which have various shortcomings. The effects of the Fe2O3 NPs and a chelated-Fe fertilizer (ethylenediaminetetraacetic acid-Fe; EDTA-Fe) fertilizer on the growth and development of peanut (Arachis hypogaea), a crop that is very sensitive to Fe deficiency, were studied in a pot experiment. The results showed that Fe2O3 NPs increased root length, plant height, biomass, and SPAD values of peanut plants. The Fe2O3 NPs promoted the growth of peanut by regulating phytohormone contents and antioxidant enzyme activity. The Fe contents in peanut plants with Fe2O3 NPs and EDTA-Fe treatments were higher than the control group. We used energy dispersive X-ray spectroscopy (EDS) to quantitatively analyze Fe in the soil. Peanut is usually cultivated in sandy soil, which is readily leached of fertilizers. However, the Fe2O3 NPs adsorbed onto sandy soil and improved the availability of Fe to the plants. Together, these results show that Fe2O3 NPs can replace traditional Fe fertilizers in the cultivation of peanut plants. To the best of our knowledge, this is the first research on the Fe2O3 NPs as the iron fertilizer. PMID:27375665

  20. Genetic analysis of some agronomic traits in groundnut (Arachis hypogaea L.

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    M.K. Alam

    2013-12-01

    Full Text Available A 10×10 half diallel experiment was conducted on groundnut (Arachis hypogaea L. to ascertain the gene action and genetic parameters of ten traits including 50% flowering, no. of pods per plant, plant height, harvest index, pod index, 100 pod weight, 100 kernel weight, pod size, diseases infection and yield per plot. The experiments were carried out in the Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU, Mymensingh during the cropping season of 2010-2011. The estimates of gene effects indicated that significance of both additive and non-additive variance for pod size, 100 pod weight and diseases infection among the traits and presence of over dominance satisfying assumptions of diallel except dormancy. However, both the additive and non-additive gene affects together importance to control of most quantitative traits in the groundnut. The average degree of dominance (H1/D 1/2 (H1 = dominance variance, D = additive variance was higher than one, indicating over dominance for all the traits. The narrow-sense heritability was high for 50% flowering (38%, harvest index (35%, pod size (52%, 100 pod weight (35% and yield per plot (41% indicating that great genetic gain could be achieved for them.

  1. Induction of Dormancy in Spanish Groundnut Seeds (Arachis hypogaea L) Using Cobalt-60 Gamma Irradiation

    International Nuclear Information System (INIS)

    Irradiation has been used in several countries to create genetic variability in groundnut (Arachis hypogaea L). Several mutated lines were isolated. Our research aims are to induce genetic variability to make a selection of an improved local population of groundnut especially with regard to the dormancy characteristic of the seeds. In this context, dried seeds (14% moisture content) of four Spanish-type local groundnut populations were treated with the dose range of 50 to 450Gy in order to study their radiosensitivity at the laboratory level. The optimal irradiation doses were determined for two groundnut populations Berrihane (P1) and Tonga oust (P3). The measurement of field agronomic characters allowed us to choose a single population that was investigated during two generations. The obtained results have shown a significant effect of irradiation through statistical analyses. Concerning the seed dormancy tested on every M2 plant, the obtained results demonstrated the existence of such a feature. However, one has to wait for the next generations in order to evaluate the evolution of the dormancy characteristics with respect to time. (author)

  2. Regeneration and acclimatization of salt-tolerant arachis hypogaea plants through tissue culture

    International Nuclear Information System (INIS)

    Excised embryos of Arachis hypogaea were cultured on Murashige and Skoog's medium (MS medium) supplemented with different combinations of growth hormones. The highest frequency of callus proliferation (80%) was recorded on MS medium mixed with 1.0 mg/1 of 2,4-D and 0.5 mg/1 of BAP. These cultures were treated with 0.65 mg/l of trans-4-hydroxy-L-proline (HyP) a:1d various concentrations (0.1-0.5%) of NaCl. In all cases the presence of salt reduced the fresh mass of callus. Shoot regeneration in the cultures took place when transferred to MS medium supplemented with 1.0 mg/1 of kinetin (Kin) and 0.5 mg/1 of 6-benzyl aminopurine (BAP). Percentage of shoot regeneration decreased with the increase of NaCl (0.1- 0.5%) in the shoot regeneration medium. Root formation in these cultures took place when the cultures were nurtured on MS medium free of growth hormones. Regeneration, hardening and acclimatization of the salt tolerant plants was conducted. (author)

  3. Cloning of Acyl-ACP Thioesterase FatA from Arachis hypogaea L. and Its Expression in Escherichia coli

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    Gao Chen

    2012-01-01

    Full Text Available In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3′-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50–70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.

  4. Isolation and expression analysis of glycerol-3-phosphate acyltransferase genes from peanuts (Arachis hypogaea L.

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    Chi, X.

    2015-09-01

    Full Text Available sn-Glycerol-3-phosphate acyltransferase (GPAT catalyzes the committed step in the production of glycerolipids. The functions of GPAT genes have been intensively studied in Arabidopsis, but not in peanuts (Arachis hypogaea L.. In this study, six AhGPAT genes were isolated from peanuts. Quantitative real-time RT-PCR analysis indicated that the AhGPAT9 transcript was more abundant in the stems, flowers, and seeds, whereas the transcript abundances of five other genes were higher in the leaves or flowers than in the other tissues examined. During seed development, the transcript levels of AhGPAT9 gradually increased, whereas the transcript levels of the other five genes decreased. In addition, the levels of AhGPAT2 transcript were distinctly enhanced after exposure to all four kinds of stress treatments except for ABA-treated leaves. The transcripts of AhGPAT1, AhGPAT6, AhGPAT8 and AhATS1 increased substantially in roots exposed to salt, drought, and ABA stress. The expressions of AhGPAT6, AhGPAT8, AhGPAT9 and AhATS1 were slightly higher in leaves under certain stress conditions than under normal conditions. The present study provides significant information for modifying oil deposition and improving the abiotic stress resistance of peanuts through molecular breeding.La aciltransferasa sn-glicerol-3-fosfato (ATGP cataliza el comprometido paso de la producción de glicerolípidos. Las funciones de los genes AhATGP se han estudiado intensivamente en Arabidopsis, pero no en cacahuete (Arachis hypogaea L.. En este estudio, seis genes AhATGP se aislaron a partir de cacahuetes. El análisis a tiempo real RT-PCR cuantitativa indicó que la transcripción AhATGP9 fue más abundante en tallos, flores y semillas, mientras que la abundancia de la transcripción de los otros cinco genes fueron mayores en hojas o flores que en los otros tejidos examinados. Durante el desarrollo de la semilla, los niveles de transcripción de AhATGP9 aumentaron gradualmente

  5. Nutrient composition of five varieties of commonly consumed Nigerian groundnut (Arachis hypogaea L.

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    Tayo, G. O.

    2012-03-01

    Full Text Available The nutrient composition of the five major varieties of groundnut (Arachis hypogaea L. commonly consumed in the south-western part of Nigeria was investigated. Raw dryshelled samples were analyzed for proximate (moisture, ash, protein, fat, fiber and carbohydrate, ‘vitamins’ (β-carotene, thiamine, niacin and tocopherol and minerals (Na, K, Ca, P, Mg, Fe, Mn, Zn, Cu, Se, Co, Al, As, Cd and Pb. Results showed that the groundnuts had 4.12-9.26% moisture, 2.77-3.31% ash, 24.26-26.35% protein, 45.41-48.14% fat, 2.51-2.94% fiber and 15.90-17.75% carbohydrate. All the varieties analyzed showed β-carotene (63.32-65.35mg/100g, thiamin (0.73-0.98mg/100g, niacin (14.00-16.03mg/100g and tocopherol (18.62-21.07mg/100g activities; with boro red having significantly (P P> Mg> Ca> Mn> Cu> Na> Zn> Fe> Al> Se in most of the varieties. Boro red also had the highest elemental contents in most of the minerals analyzed. Thus, these groundnuts can be considered useful foodstuffs in minimizing proteinenergy malnutrition (PEM and micronutrient deficiencies in Nigeria. However, the boro red variety is most recommended. The outcome of this research is a contribution to the food composition table.Se ha investigado la composición en nutrientes de las cinco principales variedades de maní (Arachis hypogaea L. de consumo habitual en la parte sur-occidental de Nigeria. A las muestras crudas con cáscara y secas se les analizó su composición proximal (humedad, ceniza, proteína, grasa, fibra e hidratos de carbono, vitaminas (β-caroteno, tiamina, niacina y tocoferol y minerales (Na, K, Ca, P, Mg, Fe, Mn, Zn, Cu, Se, Co, Al, As, Cd y Pb. Los resultados mostraron que el maní tenía entre 4.12 - 9.26% de humedad, 2.77- 3.31% de cenizas, 24.26 - 26.35% de proteína, 45.41 - 48.14% de materia grasa, 2.51 - 2.94% de fibra y 15.90 -17.75% de carbohidratos. Todas las variedades analizadas contenían β-caroteno (63.32-65.35mg/100g, tiamina (0.73-0.98mg/100g, niacina (14

  6. Induced Mutagenesis for Oil Quality Enhancement in Peanut (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    Increasing the ratio of oleic to linoleic acid (O/L) in peanut (Arachis hypogaea L.) significantly improves the nutritional and quality attributes of the crop. The lack of sufficient genetic variation in fatty acid composition, particularly the O/L ratio, in peanut germplasm and presently grown cultivars make the creation of such variability necessary. Mutation breeding of peanut was initiated with the objective of identifying stable peanut mutants with altered fatty acid composition for improved oxidative stability and nutritional quality. Seeds of peanut cultivars 'GPBD-4' and 'TPG-41' were treated with γ-radiation and/or ethyl methane sulphonate (EMS). Randomly selected mutants were advanced based on single plant selection up to the M4 generation and the harvest of M4 plants was evaluated for fatty acid composition by gas chromatography. Highly significant variation for palmitic, stearic, oleic, linoleic and arachidic acid was observed. EMS (0.5%) and 200Gy treatments were found to be effective in increasing the variability in fatty acid content in GPBD-4 and TPG-41, respectively. The variability was skewed towards high levels of oleic acid (38-67%) and low levels of linoleic acid (15-41%). Mutants selected for improved oil quality were significantly superior for O/L ratio and had reduced palmitic acid. Oil with reduced palmitic acid and increased O/L ratio is desired nutritionally. Hence, these mutants can be exploited for the improvement of oil quality. The mutants GE-87 and T3-105 recorded the highest O/L ratios, of 4.30 and 3.91, compared to control values of 1.75 and 2.60, respectively. A significant negative correlation between oleic acid and linoleic acid, palmitic acid and iodine values, and weak inverse relationship with oil content indicates the possibility of selection for improved fatty acid composition. These high oleic acid lines could be utilized further in breeding programmes for improvement of peanut oil quality. (author)

  7. Induced mutagenesis for oil quality enhancement in peanut (Arachis hypogaea l.)

    International Nuclear Information System (INIS)

    Increasing the ratio of oleic to linoleic acid (O/L) in peanut (Arachis hypogaea L) significantly improves the nutritional and quality attributes of the crop. The lack of sufficient genetic variation for fatty acid profile, particularly O/L ratio in peanut germplasm and presently grown cultivars has necessitated the creation of variability. Mutation breeding of peanut was therefore initiated with the objective of identifying stable peanut mutants with altered fatty acid composition for improved oxidative stability and nutritional quality. Seeds of peanut cultivars 'GPBD-4' and 'TPG-41' were treated with γ-radiation and/or ethyl methane sulphonate (EMS). Randomly selected mutants were advanced based on single plant selection up to M4 generation and the harvest of M4 plants was evaluated for fatty acid composition by gas chromatography. Highly significant variation for palmitic, stearic, oleic, linoleic and arachidic acid was observed. EMS (0.5%) and 200Gy treatment were found to be effective in increasing the variability for fatty acid content in GPBD-4 and TPG-41 respectively. The variability was skewed towards high levels of oleic (38-66.58%) and low levels of linoleic acid (15-41%). Mutants with improved oil quality selected were significantly superior for O/L ratio and had reduced palmitic acid. Oil with reduced palmitic acid and increased O/L ratio is nutritionally desired. Hence these mutants can be exploited in improvement of oil quality. The mutant GE-87 and T3-105, recorded highest O/L ratio of 4.30 and 3.91 as against control value of 1.75 and 2.60 respectively. A significant negative correlation between oleic acid and linoleic acid, palmitic acid and iodine value and weak inverse relationship with oil content indicates the possibility of selection for improved fatty acid composition. These high oleic acid lines could be utilized further in breeding programs for improvement of peanut oil quality. (author)

  8. Effect of neutron irradiation on somatic embryogenesis from embryonic leaflets of peanut (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    The effect of neutron irradiation on somatic embryogenesis in embryonic leaflet culture of peanut (Arachis hypogaea L.) was studied. The embryonic leaflets separated from seeds of peanut cultivar Luhua 11, which were irradiation at dosages of 0, 9.7, 14.0 and 18.0 Gy, were incubated on MSB5 medium containing 10.0 mg/L 2, 4-D to induce somatic embryos. After 4 weeks culture, the leaflets were transferred to MSB5 medium supplemented with 4.0 mg/L BAP. The embryonic leaflets from the unirradiated seeds formed somatic embryos directly on induction medium containing 10.0 mg/L 2, 4-D. However, after irradiation most of embryonic leaflets formed calli, when transferred to MSB5 medium containing 4.0 mg/L BAP, some of them formed somatic embryos. Only a few of them formed somatic embryos directly on induction medium containing 10.0 mg/L 2, 4-D. The somatic embryo formation rates varied with different dosages of neutron irradiation. The percentage of somatic embryo formation declined with the increase of neutron irradiation dosages. When the irradiation dosages were increased from 9.7 Gy to 14.0 and 18.0 Gy, the percentage of somatic embryo formation declined from about 55% to 40% and 30%, respectively. The unirradiated embryonic leaflets gave a higher rate of somatic embryo formation (above 80%). The results showed that neutron irradiation was efficient to induce genetic mutation in peanut and the optimum dosage was about 14.0 Gy. (authors)

  9. Batch Scale Removal of an Organic Pollutant Amaranth Dye from Aqueous Solution using Pisum sativum Peels and Arachis hypogaea Shells as Adsorbents

    International Nuclear Information System (INIS)

    The goal of this study was to utilize low cost and environmentally friendly adsorbents for batch scale removal of Amaranth dye from aqueous medium. Peels of Pisum sativum (Pea) and Arachis hypogaea (Peanut) were utilized to investigate their dye removing capacity. The optimized adsorption conditions for Pisum sativum (P.S.P) and Arachis hypogaea (A.H.S) were: adsorbent dose; 0.6 and 0.4 g, contact time; 45 and 10 minutes, pH; 2.0 for both, agitation speed; 150 and 100 rpm and temperature; 60 and 50 degree C for P.S.P and A.H.S respectively. The adsorption data well suited to Langmuir isotherm. Maximum adsorption capacities were found to be 144.93 and 10.53 mg/g for P.S.P and A.H.S respectively. Feasibility of the process was indicated by negative values of thermodynamic parameters delta G/sup 0/ for both adsorbents. Kinetic studies indicated that adsorption of Amaranth dye from aqueous medium by Pisum sativum peels and Arachis hypogaea shells followed pseudo-seconder order kinetics. It was concluded that Pisum sativum peels are more effective adsorbent for removal of Amaranth from aqueous solution as compared to Arachis hypogaea shells. (author)

  10. Evaluation of silver nanoparticles toxicity of Arachis hypogaea peel extracts and its larvicidal activity against malaria and dengue vectors.

    Science.gov (United States)

    Velu, Kuppan; Elumalai, Devan; Hemalatha, Periaswamy; Janaki, Arumugam; Babu, Muthu; Hemavathi, Maduraiveeran; Kaleena, Patheri Kunyil

    2015-11-01

    Silver nanoparticles (AgNPs) were successfully synthesised from aqueous silver nitrate using the extracts of Arachis hypogaea peels. The synthesised SNPs were characterized by Fourier transform-infrared spectroscopy analysis, X-ray diffraction, transmission electron microscopy analysis and high-resonance scanning electron microscopy, and energy dispersive X-ray spectroscopy. AgNPs were well defined and measured 20 to 50 nm in size. The nanoparticles were crystallized with a face-centered cubic structure. Larvicidal activity of synthesised AgNPs from A. hypogaea peels was tested for their larvicidal activity against the fourth instar larvae of Aedes aegypti (Yellow fever), Anopheles stephensi (Human malaria). The results suggest that the synthesised AgNPs have the potential to be used as an ideal eco-friendly resource for the control of A. aegypti and A. stephensi. This study provides the first report on the mosquito larvicidal activity of synthesised AgNPs from A. hypogaea peels against vectors of malaria and dengue. PMID:26154036

  11. A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L. genome

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    Li Shaoxiong

    2010-01-01

    Full Text Available Abstract Background The construction of genetic linkage maps for cultivated peanut (Arachis hypogaea L. has and continues to be an important research goal to facilitate quantitative trait locus (QTL analysis and gene tagging for use in a marker-assisted selection in breeding. Even though a few maps have been developed, they were constructed using diploid or interspecific tetraploid populations. The most recently published intra-specific map was constructed from the cross of cultivated peanuts, in which only 135 simple sequence repeat (SSR markers were sparsely populated in 22 linkage groups. The more detailed linkage map with sufficient markers is necessary to be feasible for QTL identification and marker-assisted selection. The objective of this study was to construct a genetic linkage map of cultivated peanut using simple sequence repeat (SSR markers derived primarily from peanut genomic sequences, expressed sequence tags (ESTs, and by "data mining" sequences released in GenBank. Results Three recombinant inbred lines (RILs populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Of the 901 primer pairs, 146, 124 and 64 primer pairs (markers were polymorphic in these populations, respectively, and used in genotyping these RIL populations. Individual linkage maps were constructed from each of the three populations and a composite map based on 93 common loci were created using JoinMap. The composite linkage maps consist of 22 composite linkage groups (LG with 175 SSR markers (including 47 SSRs on the published AA genome maps, representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Segregation distortion in the 3 populations was 23.0%, 13.5% and 7.8% of the markers

  12. Studies of genotypic differences in nitrogen fixation in groundnut (Arachis hypogaea) and soybean (Glycine max) in Vietnam

    International Nuclear Information System (INIS)

    Varietal differences in nodule mass and dry matter production of groundnut (Arachis hypogaea) and soybean (Glycine max) varieties were determined at flowering stage. Results of these studies have shown a high variability for traits linked with nodule mass and dry matter production. Aetylene reduction assays made at the flowering stage demonstrated significant varietal differences in biological N2 fixation among bred lines and mutants induced from Senlai groundnut variety and V70 soybean cultivar, respectively. The 15N isotope dilution technique was used for screening for differences in N2 fixation ability of groundnut and soybean varieties in a greenhouse. The results indicate that the %Ndfa values as measured using an upland rice cultivar ''CH1'' as a reference crop were high for some groundnut and soybean cultivars. (author). 10 refs, 3 tabs

  13. Data in support of proteome analysis of gynophores and early swelling pods of peanut (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    Han Xia

    2015-12-01

    Full Text Available Different from most of other plants, peanut (Arachis hypogaea L. is a typical geocarpic species which flowering and forming pegs (gynophores above the ground. Pegs penetrate into soil for embryo and pod development. To investigate the molecular mechanism of geocarpy feature of peanut, the proteome profiles of aerial grown gynophores (S1, subterranean unswollen gynophores (S2, and gynophores that had just started to swell into pods (S3 were analyzed by combining 1 DE with nano LC–MS/MS approaches. The proteomic data provided valuable information for understanding pod development of peanut. The data described here can be found in the PRIDE Archive using the reference number PXD002579-81. A more comprehensive analysis of this data may be obtained from the article in BMC Plant Biology (Zhao et al., 2015 [1].

  14. Detection of S-nitrosothiol and nitrosylated proteins in Arachis hypogaea functional nodule: response of the nitrogen fixing symbiont.

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    Debasis Maiti

    Full Text Available To detect the presence of NO, ROS and RNS in nodules of crack entry legumes, we used Arachis hypogaea functional nodule. The response of two cognate partner rhizobia was compared towards NO and GSNO using S. meliloti and Bradyrhizobium sp NC921001. ROS, NO, nitrosothiol and bacteroids were detected by fluorescence microscopy. Redox enzymes and thiol pools were detected biochemically. Nitrosothiols were found to be present but ROS and NO were absent in A. hypogaea nodule. A number of S-nitrosylated proteins were also detected. The total thiol pool and most of the redox enzymes were low in nodule cytosolic extract but these were found to be high in the partner microorganisms indicating partner rhizobia could protect the nodule environment against the nitrosothiols. Both S. meliloti and Bradyrhizobium sp NC921001 were found to contain GSNO reductase. Interestingly, there was a marked difference in growth pattern between S. meliloti and Bradyrhizobium sp in presence of sodium nitroprusside (SNP and S-nitrosoglutathione (GSNO. Bradyrhizobium sp was found to be much more tolerant to NO donor compounds than the S. meliloti. In contrast, S. meliloti showed resistance to GSNO but was sensitive to SNP. Together our data indicate that nodule environment of crack entry legumes is different than the nodules of infection mode entry in terms of NO, ROS and RNS. Based on our biochemical characterization, we propose that exchange of redox molecules and reactive chemical species is possible between the bacteroid and nodule compartment.

  15. Isolation and identification of allelochemicals produced by B. sonorensis for suppression of charcoal rot of Arachis hypogaea L.

    Science.gov (United States)

    Pandya, Urja; Saraf, Meenu

    2015-05-01

    Bacillus sonorensis MBCU2 isolated from vermicompost-amended soil from Gujarat, India showed most antagonistic activity against Macrophomina phaseolina by dual culture screening. The culture supernatant of MBCU2 completely suppressed the mycelia growth of pathogen, indicating that suppression was due to the presence of allelochemicals in the culture filtrate. Results of scanning electron microscopy revealed that MBCU2 caused morphological alteration in mycelia of M. phaseolina as evident by hyphal lysis and perforation. Lipopeptides (iturin A and surfactin) produced by MBCU2 were detected and identified by MALDI-TOF-MS as well as liquid chromatography coupled with ESI-MS/MS. Pot trial studies conducted by seed bacterization with MBCU2 resulted in statistically significant increase in Arachis hypogaea L. vegetative growth parameters such as root length (91%), shoot length (252%), fresh weight (71%), dry weight (57%), number of pod (128%), and number of seed (290%) in M. phaseolina infested soil over control as well as decreased M. phaseolina disease severity. We suggest that allelochemicals production can be linked to the mechanism of protection of A. hypogaea L. from M. phaseolina by B. sonorensis MBCU2. PMID:25346523

  16. Propiedades emulsificantes y espumantes de las proteínas de harina de cacahuate (Arachis hypogaea Lineau

    Directory of Open Access Journals (Sweden)

    Fett, R.

    2007-09-01

    Full Text Available The functional properties of proteins present in peanut (Arachis hypogaea Lineau flour were studied. The influence of the pH and protein solubility on emulsifying and foaming properties of peanut flour was evaluated. The isoelectric point (Ip of these proteins was found at the pH of 4,0; and the isoelectric region between pH 3,0 and 5,0. The evaluated surface properties decreased in the isolectric region. The emulsifying activity and time stability (30 and 120 minutes significantly decreased at the pI; the emulsifying stability at the temperature of 80°C decreased in all the isoelectric region until the pH of 6,0. The foaming activity had the best values at the pH of 2,0. The correlation between protein solubility and surface properties were more significant for emulsifying properties than for foaming properties.Se estudiaron las propiedades funcionales de las proteínas presentes en la harina de cacahuate (Arachis hypogaea Lineau. Fue evaluada la influencia que tienen el pH y la solubilidad proteica sobre las propiedades emulsificantes y espumantes de las proteínas de harina de cacahuate. El punto isoeléctrico (pI de estas proteínas se encuentra a pH 4,0; mientras que la región isoeléctrica está entre los valores de pH de 3,0 y 5,0. Las propiedades de superficie evaluadas disminuyeron en la región isoeléctrica, siendo que la actividad emulsificante y la estabilidad emulsificante en los tiempos de 30 y 120 minutos disminuyeron fuertemente en el pI; las estabilidades emulsificantes en relación a la temperatura (80 ºC diminuyeron en toda la región isoeléctrica extendiéndose hasta el pH 6,0. La actividad espumante mostró sus mejores valores a pH 2,0. Las correlaciones entre la solubilidad protéica y las propiedades de superficie fueron más importantes para las propiedades emulsificantes que para las espumantes.

  17. Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea

    Directory of Open Access Journals (Sweden)

    Crouch Jonathan H

    2008-05-01

    Full Text Available Abstract Background Cultivated peanut or groundnut (Arachis hypogaea L. is the fourth most important oilseed crop in the world, grown mainly in tropical, subtropical and warm temperate climates. Due to its origin through a single and recent polyploidization event, followed by successive selection during breeding efforts, cultivated groundnut has a limited genetic background. In such species, microsatellite or simple sequence repeat (SSR markers are very informative and useful for breeding applications. The low level of polymorphism in cultivated germplasm, however, warrants a need of larger number of polymorphic microsatellite markers for cultivated groundnut. Results A microsatellite-enriched library was constructed from the genotype TMV2. Sequencing of 720 putative SSR-positive clones from a total of 3,072 provided 490 SSRs. 71.2% of these SSRs were perfect type, 13.1% were imperfect and 15.7% were compound. Among these SSRs, the GT/CA repeat motifs were the most common (37.6% followed by GA/CT repeat motifs (25.9%. The primer pairs could be designed for a total of 170 SSRs and were optimized initially on two genotypes. 104 (61.2% primer pairs yielded scorable amplicon and 46 (44.2% primers showed polymorphism among 32 cultivated groundnut genotypes. The polymorphic SSR markers detected 2 to 5 alleles with an average of 2.44 per locus. The polymorphic information content (PIC value for these markers varied from 0.12 to 0.75 with an average of 0.46. Based on 112 alleles obtained by 46 markers, a phenogram was constructed to understand the relationships among the 32 genotypes. Majority of the genotypes representing subspecies hypogaea were grouped together in one cluster, while the genotypes belonging to subspecies fastigiata were grouped mainly under two clusters. Conclusion Newly developed set of 104 markers extends the repertoire of SSR markers for cultivated groundnut. These markers showed a good level of PIC value in cultivated germplasm

  18. Gamma radiation effects at color, antioxidant capacity and fatty acid profile in peanut (Arachis hypogaea L.); Efeitos da radiacao gama na cor, capacidade antioxidante e perfil de acidos graxos em amendoim (Arachis hypogaea L.)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Adriano Costa de; Canniatti-Brazaca, Solange Guidolin; Mansi, Debora Niero; Domingues, Maria Antonia Calori [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Agroindustria, Alimentos e Nutricao; Arthur, Valter, E-mail: arthur@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2011-01-15

    Irradiation is efficient at extinction fungi contamination in peanuts. Peanuts have high biologic value protein, minerals, vitamin E, complex B, and high concentration of lipids. The objective of this research is to evaluate the gamma irradiation effect on color, total phenolic, antioxidant activity, and fatty acid profile in peanuts (Arachis hypogaea L.). Cultivars IAC-Tatu ST and IAC-Runner 886 were submitted to gamma radiation with doses of 5.0; 7.5; 10.0, and 15.0 kGy and storage at room temperature. There was no significant difference in the color of IAC-Tatu ST. However, significant difference was found in the luminosity and Chroma in IAC-Runner 886. Total fenolics differed from the control with 33.27 mg.g{sup -1} and treatment dose of 10.0 kGy with 58.60 mg.g{sup -1} in IAC-Tatu ST. This parameter not had significant difference in IAC-Runner 886 and the control with 51.59 mg.g{sup -1}. The antioxidant activity did not present significant difference with a dose of 10.0 kGy, recommended for the elimination of fungi in peanuts. The dose of 10.0 kGy showed a decrease in saturated fatty acids, increase in unsaturated fatty acids, and an increase in linolleic acid. The oleic/linoleic relation decreased justifying further research correlating storage and oxidative stability. (author)

  19. FTIR Spectrum and Antimutagenicity of Coffea arabica Pulp and Arachis hypogaea Testa In Relation to their In Vitro Antioxidant Properties

    Directory of Open Access Journals (Sweden)

    Jonathan M. Barcelo

    2015-11-01

    Full Text Available Coffee pulp (Coffea arabica and peanut testa (Arachis hypogaea are common agricultural wastes which are extensively studied as potential sources of bioactive compounds. This study attempted to determine the correlation of the antioxidant potential, lipid peroxidation inhibition and antimutagenic activities of the crude extracts of both plant samples. Quantification of phenolic compounds, flavonoids and monomeric anthocyanins and Fourier Transform Infra-red (FTIR analysis were performed to characterize bioactive compounds. Based from the results, peanut testa extract (PTE exhibited higher total antioxidant capacity (TAC compared to coffee pulp extract (CPE at 500 μg/mL but was lower than L-Ascorbic Acid (LAA. The antimutagenic activity of PTE at > 125 μg/mL was higher compared to both RPE and LAA. Mitotic indices of A. cepa treated with PTE+MMS were higher compared to groups treated with RCPE+MMS, LAA+MMS, deionized water and MMS only. Correlation analysis revealed the TAC of ripe coffee pulp extract had significant moderate correlation with its antimutagenic activity but high correlation with LPI. The LPI of CPE showed significant moderate correlation with its antimutagenic activity. In PTE, TAC shows high significant correlation with its antimutagenic property and LPI while LPI has significant moderate correlation with its antimutagenic property. The observed activity of the CPE and PTE may be attributed primarily to the phenolic compounds in both plant extracts.

  20. Some Statistical Procedures for Evaluation of the Relative Contribution for Yield Components in irradiated populations of Peanut (Arachis hypogaea L

    International Nuclear Information System (INIS)

    The present investigation was conducted at Ismaillia Research Station-Agricultural Research Center, Ismaillia Governorate, during the two successive seasons of 2000 and 2001. Two varieties of peanut (Arachis hypogaea L) namely Giza 4 and Giza 5 were treated with gamma ray doses; 10,15, 20, 25 Kr in order to induce genetic variability and to study the importance of the relative contribution of peanut yield components by employing some statistical procedures, i.e. simple correlation, multiple linear regression and stepwise regression analysis. The results showed that, there was significant positive correlation between seed yield/plant and no. of pods/plant, 100 seed weight, shelling percentage and pod yield/plant, and there was significant positive correlation between pod yield/plant and no of seed/plant,100 pod weight and 100 seed weight. The multiple linear regression analysis clearly showed that the relative contribution (R2%) of the yield components, no. of pods/plant, no. of seed/plant and shelling % were the most important variables contributing toward seed yield/plant (90.35%), meanwhile, no. of seeds/plant, shelling % and oil content % were the most important variables contributing toward pod yield/plant (82.36 %)

  1. Overexpression of Arachis hypogaea AREB1 Gene Enhances Drought Tolerance by Modulating ROS Scavenging and Maintaining Endogenous ABA Content

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    Ling Li

    2013-06-01

    Full Text Available AhAREB1 (Arachis hypogaea Abscisic-acid Response Element Binding Protein 1 is a member of the basic domain leucine zipper (bZIP-type transcription factor in peanut. Previously, we found that expression of AhAREB1 was specifically induced by abscisic acid (ABA, dehydration and drought. To understand the drought defense mechanism regulated by AhAREB1, transgenic Arabidopsis overexpressing AhAREB1 was conducted in wild-type (WT, and a complementation experiment was employed to ABA non-sensitivity mutant abi5 (abscisic acid-insensitive 5. Constitutive expression of AhAREB1 confers water stress tolerance and is highly sensitive to exogenous ABA. Microarray and further real-time PCR analysis revealed that drought stress, reactive oxygen species (ROS scavenging, ABA synthesis/metabolism-related genes and others were regulated in transgenic Arabidopsis overexpressing AhAREB1. Accordingly, low level of ROS, but higher ABA content was detected in the transgenic Arabidopsis plants’ overexpression of AhAREB1. Taken together, it was concluded that AhAREB1 modulates ROS accumulation and endogenous ABA level to improve drought tolerance in transgenic Arabidopsis.

  2. Estabilidad del Rendimiento en Semilla de 11 Genotipos de Maní (Arachis Hypogaea l. en Santa Fe de Antioquia

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    Escobar Soto Carlos

    1997-06-01

    Full Text Available Dese 1983 se vienen realizando investigaciones sobre el comportamiento agronómico del maní (Arachis hypogaea L. en el Centro Agropecuario Cotové, propiedad de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Colombia, Sede Medellín, ubicado en la zona de vida de bosque seco tropical (bs-T. Este trabajo se planeó con el objetivo de estudiar la estabilidad del rendimiento en semilla de 11 genotipos identificados como promisorios para esa zona. Estos genotipos se sembraron durante seis épocas dijeren/es y se registró el rendimiento de maní en semilla. En cada época los genotipos se dispusieron de acuerdo con el diseño de bloques completos al azar Para el estudio de la estabilidad del rendimiento se empleó el modelo propuesto por Eberhart y Russell. De acuerdo con los resultados obtenidos se encontró que el genotipo Tatuí 76 SM1CA es el que reúne las condiciones tanto de estabilidad como de calidad de semilla para ser sembrado en dicho Centro.

  3. Bioassay-guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques.

    Science.gov (United States)

    Oldoni, Tatiane L C; Melo, Priscilla S; Massarioli, Adna P; Moreno, Ivani A M; Bezerra, Rosângela M N; Rosalen, Pedro L; da Silva, Gil V J; Nascimento, Andréa M; Alencar, Severino M

    2016-02-01

    Purification and bioassay-guided fractionation were employed to isolate proanthocyanidins with antioxidant activity from peanut skin (Arachis hypogaea Runner 886). The crude extract was prepared with acetone (60% v/v) and purified using chromatographic methods, including a semipreparative HPLC technique. As a result, two proanthocyanidins were isolated and identified using NMR, epicatechin-(2 β → O → 7, 4 β → 8)-catechin (proanthocyanidin A1) and epicatechin-(β → 2 O → 7, 4 β → 8)-epicatechin (proanthocyanidin A2). Despite the structural similarity, differences were observed in their antioxidant activity. Proanthocyanidin A1 proved to be more active, with EC50 value for DPPH radical scavenging of 18.25 μg/mL and reduction of Fe(3+)-TPTZ complex of 7.59 mmol/g, higher than that of synthetic antioxidant BHT. This compound evaluated by ABTS(+) was similar to that of natural quercetin. Therefore, peanut skin is an important source of bioactive compounds that may be used as a mild antioxidant for food preservation. PMID:26304352

  4. In Vitro Inhibition of Cellulolytic Enzymes of Fusarium Oxysporum by Trichoderma spp and Pseudomonas Fluorescens on Arachis Hypogaea L

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    P. Rajeswari

    2015-03-01

    Full Text Available In an attempt to develop biocontrol system for management of Fusarium wilt in groundnut, Trichoderma viride, Trichoderma harzianum,and Pseudomonas fluorescens were evaluated for their antagonistic activity against Fusarium oxysporum in vitro. .Fusarium wilt diseasescaused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtratesof T.viride (1%, T. harzianum (1.5%, and P. fluorescens (2% on the in vitro inhibition of cellulolytic enzymes of Fusarium oxysporum. Theactivity of 1,4 endoglucanases, 1,4exoglucanase Cellobiase produced by Fusariumoxysporum was higher, when compared to control.Maximum inhibition of above Cellulolytic enzymes (1, 4 endoglucanases, 1,4exoglucanase, Cellobiase was shown by T. viride treatment wasfollowed by T. harzianum and P. fluorescens. Of all the treatments, T. viride treatment showed higher rate of inhibition of Cellulolytic enzymesof Fusarium oxysporum followed by that of T. harzianum and P. fluorescens.This present study indicates that culture filtrate of T.viride(1%is the best biocontrol agent in the inhibition of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea .L

  5. Propiedades emulsificantes y espumantes de las proteínas de harina de cacahuate (Arachis hypogaea Lineau)

    OpenAIRE

    R. FETT; Barrera Arellano, D.; Bordignon Luiz, M. T.; E. M. KUSKOSKI; Ferreyra, J. C.

    2007-01-01

    The functional properties of proteins present in peanut (Arachis hypogaea Lineau) flour were studied. The influence of the pH and protein solubility on emulsifying and foaming properties of peanut flour was evaluated. The isoelectric point (Ip) of these proteins was found at the pH of 4,0; and the isoelectric region between pH 3,0 and 5,0. The evaluated surface properties decreased in the isolectric region. The emulsifying activity and time stability (30 and 120 minutes) significantly decreas...

  6. Quantitative trait locus analysis and construction of consensus genetic map for drought tolerance traits based on three recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.)

    OpenAIRE

    Gautami, B.; M.K. Pandey; Vadez, V.; Nigam, S. N.; Ratnakumar, P.; L. Krishnamurthy; T. Radhakrishnan; M. V. C. Gowda; Narasu, M. L.; Hoisington, D. A.; Knapp, S. J.; Varshney, R.K.

    2011-01-01

    Groundnut (Arachis hypogaea L.) is an important food and cash crop grown mainly in semi-arid tropics (SAT) regions of the world where drought is the major constraint on productivity. With the aim of understanding the genetic basis and identification of quantitative trait loci (QTL) for drought tolerance, two new recombinant inbred line (RIL) mapping populations, namely ICGS 76 × CSMG 84-1 (RIL-2) and ICGS 44 × ICGS 76 (RIL-3), were used. After screening of 3,215 simple sequence repeat (SSR) m...

  7. Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut (Arachis hypogaea L.)

    OpenAIRE

    Ravi, K.; Vadez, V.; Isobe, S.; Mir, R. R.; Y. Guo; Nigam, S. N.; M. V. C. Gowda; T. Radhakrishnan; Bertioli, D J; Knapp, S. J.; Varshney, R.K.

    2010-01-01

    Cultivated groundnut or peanut (Arachis hypogaea L.), an allotetraploid (2n = 4x = 40), is a self pollinated and widely grown crop in the semi-arid regions of the world. Improvement of drought tolerance is an important area of research for groundnut breeding programmes. Therefore, for the identification of candidate QTLs for drought tolerance, a comprehensive and refined genetic map containing 191 SSR loci based on a single mapping population (TAG 24 × ICGV 86031), segregating for drought and...

  8. The Peanut (Arachis hypogaea L. Gene AhLPAT2 Increases the Lipid Content of Transgenic Arabidopsis Seeds.

    Directory of Open Access Journals (Sweden)

    Silong Chen

    Full Text Available Lysophosphatidic acid acyltransferase (LPAT, which converts lysophosphatidic acid (LPA to phosphatidic acid (PA, catalyzes the addition of fatty acyl moieties to the sn-2 position of the LPA glycerol backbone in triacylglycerol (TAG biosynthesis. We recently reported the cloning and temporal-spatial expression of a peanut (Arachis hypogaea AhLPAT2gene, showing that an increase in AhLPAT2 transcript levels was closely correlated with an increase in seed oil levels. However, the function of the enzyme encoded by the AhLPAT2 gene remains unclear. Here, we report that AhLPAT2 transcript levels were consistently higher in the seeds of a high-oil cultivar than in those of a low-oil cultivar across different seed developmental stages. Seed-specific overexpression of AhLPAT2 in Arabidopsis results in a higher percentage of oil in the seeds and greater-than-average seed weight in the transgenic plants compared with the wild-type plants, leading to a significant increase in total oil yield per plant. The total fatty acid (FA content and the proportion of unsaturated FAs also increased. In the developing siliques of AhLPAT2-overexpressing plants, the expression levels of genes encoding crucial enzymes involved in de novo FA synthesis, acetyl-CoA subunit (AtBCCP2 and acyl carrier protein 1 (AtACP1 were elevated. AhLPAT2 overexpression also promoted the expression of several key genes related to TAG assembly, sucrose metabolism, and glycolysis. These results demonstrate that the expression of AhLPAT2 plays an important role in glycerolipid production in peanuts.

  9. Identification and Evaluation of Single-Nucleotide Polymorphisms in Allotetraploid Peanut (Arachis hypogaea L.) Based on Amplicon Sequencing Combined with High Resolution Melting (HRM) Analysis.

    Science.gov (United States)

    Hong, Yanbin; Pandey, Manish K; Liu, Ying; Chen, Xiaoping; Liu, Hong; Varshney, Rajeev K; Liang, Xuanqiang; Huang, Shangzhi

    2015-01-01

    The cultivated peanut (Arachis hypogaea L.) is an allotetraploid (AABB) species derived from the A-genome (Arachis duranensis) and B-genome (Arachis ipaensis) progenitors. Presence of two versions of a DNA sequence based on the two progenitor genomes poses a serious technical and analytical problem during single nucleotide polymorphism (SNP) marker identification and analysis. In this context, we have analyzed 200 amplicons derived from expressed sequence tags (ESTs) and genome survey sequences (GSS) to identify SNPs in a panel of genotypes consisting of 12 cultivated peanut varieties and two diploid progenitors representing the ancestral genomes. A total of 18 EST-SNPs and 44 genomic-SNPs were identified in 12 peanut varieties by aligning the sequence of A. hypogaea with diploid progenitors. The average frequency of sequence polymorphism was higher for genomic-SNPs than the EST-SNPs with one genomic-SNP every 1011 bp as compared to one EST-SNP every 2557 bp. In order to estimate the potential and further applicability of these identified SNPs, 96 peanut varieties were genotyped using high resolution melting (HRM) method. Polymorphism information content (PIC) values for EST-SNPs ranged between 0.021 and 0.413 with a mean of 0.172 in the set of peanut varieties, while genomic-SNPs ranged between 0.080 and 0.478 with a mean of 0.249. Total 33 SNPs were used for polymorphism detection among the parents and 10 selected lines from mapping population Y13Zh (Zhenzhuhei × Yueyou13). Of the total 33 SNPs, nine SNPs showed polymorphism in the mapping population Y13Zh, and seven SNPs were successfully mapped into five linkage groups. Our results showed that SNPs can be identified in allotetraploid peanut with high accuracy through amplicon sequencing and HRM assay. The identified SNPs were very informative and can be used for different genetic and breeding applications in peanut. PMID:26697032

  10. Efeitos da radiação gama na cor, capacidade antioxidante e perfil de ácidos graxos em amendoim (Arachis hypogaea L.)

    OpenAIRE

    Adriano Costa de Camargo; Solange Guidolin Canniatti-Brazaca; Débora Niero Mansi; Maria Antonia Calori Domingues; Valter Arthur

    2011-01-01

    A irradiação gama é eficiente para eliminar a contaminação fúngica em grãos de amendoim. Este apresenta proteínas de alto valor biológico, minerais, vitaminas E, complexo B e alta concentração de lipídios. Este trabalho teve como objetivo avaliar o efeito da aplicação de radiações gama na cor, fenólicos totais, atividade antioxidante e perfil de ácidos graxos em amendoim (Arachis hypogaea L.). Os cultivares IAC-Tatu ST e IAC-Runner 886 foram submetidos a radiações gama com doses de 5,0; 7,5; ...

  11. Ação do trifluralin na micorrização e crescimento de plantas de amendoim (Arachis hypogaea Action of trifluralin on mycorrhization and growth of peanut (Arachis hypogaea plants

    Directory of Open Access Journals (Sweden)

    M.F.S.P. Peixoto

    2010-01-01

    Full Text Available O amendoim (Arachis hypogaea é uma planta que durante as fases iniciais do seu desenvolvimento sofre mais intensamente a competição das plantas daninhas, prejudicando sensivelmente sua produção. Uma forma muito eficiente de solucionar esse problema é o uso do herbicida trifluralin; no entanto, seu uso pode prejudicar a micorrização, uma associação benéfica formada por fungos e raízes da planta hospedeira. Este trabalho objetivou avaliar o efeito do herbicida trifluralin e de duas espécies de fungos micorrízicos (Gigaspora margarita e Acaulospora scrubiculata na micorrização e no crescimento inicial de plantas de amendoim. O trabalho foi realizado na casa de vegetação do Centro de Ciências Agrárias, Ambientais e Biológicas da Universidade Federal do Recôncavo da Bahia, no município de Cruz das Almas - BA. Utilizou-se o cultivar de amendoim Vagem Lisa. Os tratamentos testados envolveram aplicação (C/H ou não (S/H de herbicida e inoculação individual de dois fungos micorrízicos: Gigaspora margarita (F1 e Acaulospora scrubiculata (F2, sendo os seguintes: S/H + F1, S/H + F2, C/H + F1, C/H + F2, C/H S/F (sem fungo e S/H S/F. O delineamento experimental foi inteiramente ao acaso, em esquema fatorial (2 x 2 + 2, com quatro repetições. Os resultados mostraram que a colonização micorrízica para Gigaspora margarita foi de 63% e, quando se aplicou o herbicida, de 44%; Acaulospora scrubiculata apresentou baixo índice de colonização (5,75% e 1%, sem e com herbicida, respectivamente; a eficiência micorrízica foi superior na associação com G. margarita; a dependência micorrízica foi aumentada na presença do herbicida para as duas espécies de fungo inoculadas; a altura da planta, o volume de raízes, a massa seca da parte aérea e das raízes e a massa seca da planta foram superiores aos dos demais tratamentos. Conclui-se que o herbicida trifluralin prejudica a micorrização e o crescimento inicial das plantas de

  12. Cruzamentos entre Arachis hypogaea e as espécies A. diogoi e A. spp (30006, 30035 Interspecific crosses between Arachis hypogaea and the species A. diogoi and A. spp (30006, 30035

    Directory of Open Access Journals (Sweden)

    Antonio Sidney Pompeu

    1983-01-01

    Full Text Available Artificial crosses were made between A. hypogaea, using lines of 'Tatu', 'Roxo', Tatuí x Roxo 80-1 and the cultivar Tatuí as female parents and introduced plants of items 30001 and 30005 reported as belonging to A. diogoi and of 30006 and 30035 of A. spp, under greenhouse conditions. From the pollinated flowers of A. hypogaea x A. diogoi, 53.4 and 45.2% gave rise to pegs and fruits with normal seeds, respectively. The amount of viable pollen grains ranged from 4.8 to 13.4 with an average of 8.3% in this cross compared to 97.2 and 95.7% observed for A. hypogaea and A. diogoi. In the crosses A. hypogaea x A. sp. 30006 and A. hypogaea x A. sp. 30035, 13 and 9 normal fruits with 22 and 15 viable seeds were obtained, respectively. The pollen viability varied from 3.5 to 15 with mean of 10% in the former cross and was around 9.9% in the latter, compared with 97.8 and 98.2% noted for A. sp. 30006 and for A. sp. 30035. These results showed that plants previously classified as A. diogoi belong to another species. They also demonstrated the possibilities of using A. diogoi, A. sp. 30006 and A. sp. 30035 in the peanut genetic improvement.

  13. Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut (Arachis hypogaea L.).

    Science.gov (United States)

    Ravi, K; Vadez, V; Isobe, S; Mir, R R; Guo, Y; Nigam, S N; Gowda, M V C; Radhakrishnan, T; Bertioli, D J; Knapp, S J; Varshney, R K

    2011-04-01

    Cultivated groundnut or peanut (Arachis hypogaea L.), an allotetraploid (2n = 4x = 40), is a self pollinated and widely grown crop in the semi-arid regions of the world. Improvement of drought tolerance is an important area of research for groundnut breeding programmes. Therefore, for the identification of candidate QTLs for drought tolerance, a comprehensive and refined genetic map containing 191 SSR loci based on a single mapping population (TAG 24 x ICGV 86031), segregating for drought and surrogate traits was developed. Genotyping data and phenotyping data collected for more than ten drought related traits in 2-3 seasons were analyzed in detail for identification of main effect QTLs (M-QTLs) and epistatic QTLs (E-QTLs) using QTL Cartographer, QTLNetwork and Genotype Matrix Mapping (GMM) programmes. A total of 105 M-QTLs with 3.48-33.36% phenotypic variation explained (PVE) were identified using QTL Cartographer, while only 65 M-QTLs with 1.3-15.01% PVE were identified using QTLNetwork. A total of 53 M-QTLs were such which were identified using both programmes. On the other hand, GMM identified 186 (8.54-44.72% PVE) and 63 (7.11-21.13% PVE), three and two loci interactions, whereas only 8 E-QTL interactions with 1.7-8.34% PVE were identified through QTLNetwork. Interestingly a number of co-localized QTLs controlling 2-9 traits were also identified. The identification of few major, many minor M-QTLs and QTL × QTL interactions during the present study confirmed the complex and quantitative nature of drought tolerance in groundnut. This study suggests deployment of modern approaches like marker-assisted recurrent selection or genomic selection instead of marker-assisted backcrossing approach for breeding for drought tolerance in groundnut. PMID:21191568

  14. Quantitative trait locus analysis and construction of consensus genetic map for drought tolerance traits based on three recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.).

    Science.gov (United States)

    Gautami, B; Pandey, M K; Vadez, V; Nigam, S N; Ratnakumar, P; Krishnamurthy, L; Radhakrishnan, T; Gowda, M V C; Narasu, M L; Hoisington, D A; Knapp, S J; Varshney, R K

    2012-08-01

    Groundnut (Arachis hypogaea L.) is an important food and cash crop grown mainly in semi-arid tropics (SAT) regions of the world where drought is the major constraint on productivity. With the aim of understanding the genetic basis and identification of quantitative trait loci (QTL) for drought tolerance, two new recombinant inbred line (RIL) mapping populations, namely ICGS 76 × CSMG 84-1 (RIL-2) and ICGS 44 × ICGS 76 (RIL-3), were used. After screening of 3,215 simple sequence repeat (SSR) markers on the parental genotypes of these populations, two new genetic maps were developed with 119 (RIL-2) and 82 (RIL-3) SSR loci. Together with these maps and the reference map with 191 SSR loci based on TAG 24 × ICGV 86031 (RIL-1), a consensus map was constructed with 293 SSR loci distributed over 20 linkage groups, spanning 2,840.8 cM. As all these three populations segregate for drought-tolerance-related traits, a comprehensive QTL analysis identified 153 main effect QTL (M-QTL) and 25 epistatic QTL (E-QTL) for drought-tolerance-related traits. Localization of these QTL on the consensus map provided 16 genomic regions that contained 125 QTL. A few key genomic regions were selected on the basis of the QTL identified in each region, and their expected role in drought adaptation is also discussed. Given that no major QTL for drought adaptation were identified, novel breeding approaches such as marker-assisted recurrent selection (MARS) and genomic selection (GS) approaches are likely to be the preferred approaches for introgression of a larger number of QTL in order to breed drought-tolerant groundnut genotypes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9660-0) contains supplementary material, which is available to authorized users. PMID:22924017

  15. Application of targeted metagenomics to explore abundance and diversity of CO₂-fixing bacterial community using cbbL gene from the rhizosphere of Arachis hypogaea.

    Science.gov (United States)

    Yousuf, Basit; Keshri, Jitendra; Mishra, Avinash; Jha, Bhavanath

    2012-09-10

    Sequestration of CO(2) by autotrophic bacteria is a key process of biogeochemical carbon cycling in soil ecosystem. Rhizosphere is a rich niche of microbial activity and diversity, influenced by change in atmospheric CO(2). Structural changes in rhizosphere composition influence microbial communities and the nutrient cycling. In the present study, the bacterial diversity and population dynamics were established using cbbL and 16S rRNA gene targeted metagenomics approach from the rhizosphere of Arachis hypogaea. A total of 108 cbbL clones were obtained from the rhizospheric soil which revealed predominance of cbbL sequences affiliated to Rhizobium leguminosarum, Bradyrhizobium sp., Sinorhizobium meliloti, Ochrobactrum anthropi and a variety of uncultured cbbL harboring bacteria. The 16S rRNA gene clone library exhibited the dominance of Firmicutes (34.4%), Proteobacteria (18.3%), Actinobacteria (17.2%) and Bacteroidetes (16.1%). About 43% nucleotide sequences of 16S rRNA gene clone library were novel genera which showed <95% homology with published sequences. Gene copy number of cbbL and 16S rRNA genes, determined by quantitative real-time PCR (qRT PCR), was 9.38 ± 0.75 × 10(7) and 5.43 ± 0.79 × 10(8) (per g dry soil), respectively. The results exhibited bacterial community structure with high bacterial diversity and abundance of CO(2)-fixing bacteria, which can be explored further for their role in carbon cycling, sustainable agriculture and environment management. PMID:22766402

  16. Características agronómicas y sanidad de germoplasma promisorio de maní (Arachis hypogaea L. en Quevedo, Ecuador

    Directory of Open Access Journals (Sweden)

    Felipe Rafael Garcés-Fiallos

    2014-12-01

    Full Text Available Durante las épocas secas de 2010 y 2011, en la finca experimental La María de la Universidad Técnica Estatal de Quevedo, Ecuador, localizada en el km. 7.5 vía Quevedo-El Empalme, a 79° 30' 08" O y 01° 00' 35" S, se evaluaron las características agronómicas y las condiciones sanitarias de líneas y variedades de maní (Arachis hypogaea L.. Se utilizaron las líneas promisorias de maní CB-02, CB-05, CB-12, CB-13, CB-15, CB-16 y CB-23 de la Universidad y como testigos las variedades comerciales Diamante, Polachi, INIAP-380 e INIAP-381. Se midieron la emergencia de plántulas, días a floración, altura de planta, algunos componentes productivos (número de granos, frutos llenos y estériles, peso de frutos y granos y la presencia de cercosporiosis (Cercospora arachidicola y Cercosporidium personatum y roya (Puccinia arachidis. Se utilizó un diseño de bloques completos al azar (DBCA con 11 tratamientos y tres repeticiones en 2010 y cuatro repeticiones en 2011. Para las comparaciones entre las medias de los tratamientos se empleó la prueba de rangos múltiples de Duncan (P < 0.05. La línea CB-23 se destacó entre el germoplasma promisorio por su mayor productividad; esta misma línea junto con la variedad comercial INIAP-380, presentaron los menores ataques de cercosporiosis. La línea CB-15 y la variedad Polachi no presentaron lesiones de la roya.

  17. Genetic transformation of peanut (Arachis hypogaea L.) using cotyledonary node as explant and a promoterless gus::nptII fusion gene based vector

    Indian Academy of Sciences (India)

    T Swathi Anuradha; S K Jami; R S Datla; P B Kirti

    2006-06-01

    We have generated putative promoter tagged transgenic lines in Arachis hypogaea cv JL-24 using cotyledonary node (CN) as an explant and a promoterless gus::nptII bifunctional fusion gene mediated by Agrobacterium transformation. MS medium fortified with 6-benzylaminopurine (BAP) at 4 mg/l in combination with 0.1 mg/l -napthaleneacetic acid (NAA) was the most effective out of the various BAP and NAA combinations tested in multiple shoot bud formation. Parameters enhancing genetic transformation viz. seedling age, Agrobacterium genetic background and co-cultivation periods were studied by using the binary vector p35SGUSINT. Genetic transformation with CN explants from 6-dayold seedlings co-cultivated with Agrobacterium GV2260 strain for 3 days resulted in high kanamycin resistant shoot induction percentage (45%); approximately 31% transformation frequency was achieved with p35S GUSINT in -glucuronidase (GUS) assays. Among the in vivo GUS fusions studied with promoterless gus::nptII construct, GUS-positive sectors occupied 38% of the total transient GUS percentage. We have generated over 141 putative T0 plants by using the promoterless construct and transferred them to the field. Among these, 82 plants survived well in the green house and 5 plants corresponding to 3.54% showed stable integration of the fusion gene as evidenced by GUS, polymerase chain reaction (PCR) and Southern blot analyses. Twenty-four plants were positive for GUS showing either tissue-specific expression or blue spots in at least one plant part. The progeny of 15 T0 plants indicated Mendelian inheritance pattern of segregation for single-copy integration. The tissue-specific GUS expression patterns were more or less similar in both T0 and corresponding T1 progeny plants. We present the differential patterns of GUS expression identified in the putative promoter-tagged transgenic lines in the present communication.

  18. Efeitos da radiação gama na cor, capacidade antioxidante e perfil de ácidos graxos em amendoim (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    Adriano Costa de Camargo

    2011-03-01

    Full Text Available A irradiação gama é eficiente para eliminar a contaminação fúngica em grãos de amendoim. Este apresenta proteínas de alto valor biológico, minerais, vitaminas E, complexo B e alta concentração de lipídios. Este trabalho teve como objetivo avaliar o efeito da aplicação de radiações gama na cor, fenólicos totais, atividade antioxidante e perfil de ácidos graxos em amendoim (Arachis hypogaea L.. Os cultivares IAC-Tatu ST e IAC-Runner 886 foram submetidos a radiações gama com doses de 5,0; 7,5; 10,0; e 15,0 kGy e armazenados em temperatura ambiente. Não foram verificadas diferenças significativas na cor dos amendoins IAC-Tatu ST. Diferenças significativas foram detectadas para a luminosidade e o Croma do IAC-Runner 886. Os fenólicos totais diferiram significativamente entre o controle com 33,27 mg.g-1 e o tratamento com 10,0 kGy com 58,60 mg.g-1 no IAC-Tatu ST, neste parâmetro não foram observadas diferenças significativas no IAC-Runner 886, cujo controle foi 51,59 mg.g-1. Para atividade antioxidante não foi verificada diferença significativa com a dose de 10,0 kGy, recomendada para eliminação fúngica de amendoim. Na dose de 10,0 kGy, verificou-se a diminuição de ácidos graxos saturados, o aumento dos insaturados e o aumento de ácido linoleico. A relação oleico/linoleico diminuiu, justificando novos estudos correlacionando armazenamento e estabilidade oxidativa.

  19. Nitrogen use efficiency of soil and foliar application of 15N labelled urea with or without inoculation and micro nutrients by groundnut (Arachis hypogaea L) in a semi-arid environment

    International Nuclear Information System (INIS)

    The nitrogen use efficiency by groundnut (Arachis hypogaea L) grown in the field on a sandy loam soil (Plinthaqualf) in Ghana was estimated using 15 N labelled urea applied to the soil at planting on one hand and urea in an NPKS nutrient solution sprayed during the pod-filling stage of the crop on the other. The efficiency of the 15 N utilization was low and ranged from 26.2 to 27.3% when the urea was applied to the soil, and 14.3 to 20.8% with the foliar spray. The results suggest active absorption of the applied nitrogen through the root system of the groundnut at the pod-filling stage. Seed inoculation and application of Mo significantly reduced the 15 N use efficiently. (author). 5 tabs., 24 refs

  20. Efeitos do controle de plantas daninhas, com herbicidas, na produção e qualidade fisiológica de sementes de amendoim (Arachis hypogaea L. Effect of herbicides on weed control, production and physiological quality of peanut (Arachis hypogaea L. 'Tatu'

    Directory of Open Access Journals (Sweden)

    J.F. Silva

    1983-12-01

    Full Text Available Com o objetivo de avaliar o efeito de herbicidas no controle de plantas daninhas, na produção e na qualidade fisiológica das sementes de amendoim (Arachis hypogaea L. 'Tatu', foi instalado um experimento no campo, em solo Podzólico Vermelho-Amarelo Câmbico, fase terraço, com 2,8% de matéria orgânica e textura argilosa. Usaram-se os tratamentos: testemunha com capina, testemunha sem capina, trifuralin a 0,58 kg do i.a./ha; vernolate a 3,6 kg do i.a./ha; nitralin a 0,75 kg do i.a./ha; fluorodifen a 0,9 kg do i.a./ha e pendimethalin a 1,5 kg do i.a./ha. Avaliaram-se a população inicial, a produção de sementes, produção de casca, o amendoim com casca, e o número de plantas daninhas. Realizaram-se também, testes de avaliação da qualidade fisiológica das sementes de amendoim, os quais foram constituídos pelo teste-padrão de germinação após 20, 40 e 80 horas de permanência das sementes na câmara de envelhecimento precoce, que estava regulada para funcionar a 42°± 3°C e 95% de umidade relativa. No campo observou-se predominância de trevo (Oxalis sp, picão-branco (Galinsoga parviflora Cav. e tiririca (Cyperus rotundus L.. Trifuralin, fluorodifen e pendimenthalin fo-ram os herbicidas da maior eficiência no controle do trevo, enquanto para tiririca o tratamento com nitralin não diferiu da testemunha capinada, e para o picão-branco, o tratamento com pendimethalin controlou totalmente esta planta daninha. Para a produção de sementes de amendoim, não houve diferenças entre os tratamentos. A produção de casca e a produção de amendoim com casca, o tratamento com fluorodifen diferiu apenas da testemunha sem capina, suplantando-a. A ocorrência de patógenos nas sementes da testemunha sem capina, e o herbicida nitralin prejudicaram a germinação das sementes. O tratamento das sementes de amendoim na câmara de envelhecimento precoce, pelo período de 20 horas, promoveu nas sement es do tratamento testemunha sem capina e

  1. Introgression of the SbASR-1 gene cloned from a halophyte Salicornia brachiate enhances salinity and drought endurance in transgenic groundnut (arachis hypogaea)and acts as a transcription factor [corrected].

    Science.gov (United States)

    Tiwari, Vivekanand; Chaturvedi, Amit Kumar; Mishra, Avinash; Jha, Bhavanath

    2015-01-01

    The SbASR-1 gene, cloned from a halophyte Salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. The genome of S. brachiata has two paralogs of the SbASR-1 gene (2549 bp), which is comprised of a single intron of 1611 bp, the largest intron of the  abscisic acid stress ripening [ASR] gene family yet reported. In silico analysis of the 843-bp putative promoter revealed the presence of ABA, biotic stress, dehydration, phytohormone, salinity, and sugar responsive cis-regulatory motifs. The SbASR-1 protein belongs to Group 7 LEA protein family with different amino acid composition compared to their glycophytic homologs. Bipartite Nuclear Localization Signal (NLS) was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein. Furthermore, transgenic groundnut (Arachis hypogaea) plants over-expressing the SbASR-1 gene constitutively showed enhanced salinity and drought stress tolerance in the T1 generation. Leaves of transgenic lines exhibited higher chlorophyll and relative water contents and lower electrolyte leakage, malondialdehyde content, proline, sugars, and starch accumulation under stress treatments than wild-type (Wt) plants. Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions. Transcript expression of APX (ascorbate peroxidase) and CAT (catalase) genes were higher in Wt plants, whereas the SOD (superoxide dismutase) transcripts were higher in transgenic lines under stress. Electrophoretic mobility shift assay (EMSA) confirmed that the SbASR-1 protein binds at the consensus sequence (C/G/A)(G/T)CC(C/G)(C/G/A)(A/T). Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant) protein and a transcription factor. PMID:26158616

  2. Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7 and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L..

    Directory of Open Access Journals (Sweden)

    Vittal Pruthvi

    Full Text Available Drought, salinity and extreme temperatures are the most common abiotic stresses, adversely affecting plant growth and productivity. Exposure of plants to stress activates stress signalling pathways that induce biochemical and physiological changes essential for stress acclimation. Stress tolerance is governed by multiple traits, and importance of a few traits in imparting tolerance has been demonstrated. Under drought, traits linked to water mining and water conservation, water use efficiency and cellular tolerance (CT to desiccation are considered to be relevant. In this study, an attempt has been made to improve CT in drought hardy crop, peanut (Arachis hypogaea L., cv. TMV2 by co-expressing stress-responsive transcription factors (TFs, AtDREB2A, AtHB7 and AtABF3, associated with downstream gene expression. Transgenic plants simultaneously expressing these TFs showed increased tolerance to drought, salinity and oxidative stresses compared to wild type, with an increase in total plant biomass. The transgenic plants exhibited improved membrane and chlorophyll stability due to enhanced reactive oxygen species scavenging and osmotic adjustment by proline synthesis under stress. The improvement in stress tolerance in transgenic lines were associated with induced expression of various CT related genes like AhGlutaredoxin, AhAldehyde reductase, AhSerine threonine kinase like protein, AhRbx1, AhProline amino peptidase, AhHSP70, AhDIP and AhLea4. Taken together the results indicate that co-expression of stress responsive TFs can activate multiple CT pathways, and this strategy can be employed to improve abiotic stress tolerance in crop plants.

  3. Cloning and Prokaryotic Expression Character Analysis of AhRab7 Genes from Peanut(Arachis hypogaea L.)%花生AhRab7基因的克隆及其原核表达研究

    Institute of Scientific and Technical Information of China (English)

    向小华; 宋琳; 裴玉贺; 郭新梅; 隋炯明; 宋希云

    2013-01-01

    The full length eDNA of AhRab7-1 and AhRab7-2 was isolated from HuaYu 20(Arachis hypogaea L.) by RT-PCR and transferred into Escherichina coli to analyze whether the expression of the small G protein AhRab7 inE.coli was associated with the tolerance to high salt environment for E.coli.AhRab7-1 and AhRab7-2 were 872 bp and 816 bp,containing a 621 bp and 618 bp open reading frame and encoding 206 and 205 amino acids,respectively.AhR-ab7-1 and AhRab7-2 were constructed into the expression vector pET-28a(+) with full-length ORF,then transferred into E.coli.After inducing by IPTG,the E.coli were treated with high salinity stresses and the function of the protein AhRab7 could be tested.The tolerance activity assay showed that pET-28a-AhRab7-1 and pET-28a-AhRab7-2 had normal restructure enzyme activities and significantly released the salt tolerance of E.coli in LB medium with high NaC1 concentration(5.5%-10%).The control which had been transferred into the vector pET-28 did not showed the similar enzyme activity.The result suggested the expression of AhRab7 in E.coli could extremely improve the salt tolerance for E.coli.The product proteins of AhRab7-1 and AhRab7-2 were detected by SDS-PAGE and the proteins were 23 kDa as expected.Our researches could be used as a starting point for generation of plants tolerance to saline-alkali and other abiotic stress in the future.%为了研究花生小G蛋白AhRab7基因在大肠杆菌中的表达模式及与高盐胁迫的关联性,采用RT-PCR技术从花生(Arachis hypogaea L.)品种花育20中克隆了AhRab7(7-1、7-2)的cDNA全长序列,通过核苷酸序列和氨基酸序列分析结果表明AhRab7(7-1、7-2)的cDNA全长分别为872 bp、816 bp,分别含1个621 bp、618 bp大小的开放阅读框(ORF,open reading frame),拟编码氨基酸数分别为206aa、205aa.将携带完整的ORF序列连入表达载体pET-28a(+)中,经IPTG诱导后进行耐盐性测定,结果表明两种全长重组酶(分别含pET-28a-Rab7-1

  4. TAXONOMY OF THE GENUS ARACHIS (LEGUMINOSAE) Taxonomy of the genus Arachis (Leguminosae)

    OpenAIRE

    Antonio Krapovickas; Walton C. Gregory

    2010-01-01

    Pasaron casi 100 años entre la designación por Linneo de la entonces única especie de Arachis (A. hypogaea L.) conocida por los europeos, y el primer tratamiento taxonómico del
    género por Bentham en 1841. Durante los siguientes 100 años, aparecieron cinco a diez
    descripciones de especies adicionales, que asignaban diferentes especies a los mismos
  5. Characterization of a Pathogen Induced Thaumatin-Like Protein Gene AdTLP from Arachis diogoi, a Wild Peanut

    OpenAIRE

    Singh, Naveen Kumar; Kumar, Koppolu Raja Rajesh; Kumar, Dilip; Shukla, Pawan; P B Kirti

    2013-01-01

    Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the lat...

  6. CROMOSOMAS Y EVOLUCION EN ARACHIS (LEGlIMINOSAE

    Directory of Open Access Journals (Sweden)

    Aveliano Fernández

    2010-11-01

    Full Text Available

    Fueron analizadas citológicamente cuarenta y un especies pertenecientes a ocho de las nueve secciones del género Arachis. Se analizaron en detalle los cariotipos veinticuatro de las cuarenta y un especies. Todas las especies tienen un par de cromosomas con satélite, excepto A. valida, que tiene dos pares. Los satélites se clasifican en diez tipos. Las secciones Procumbentes, Erectoides, Extranervosae, Caulorrhizae, Rhizomatosae, Heteranthae y Trierectoides tienen sólo uno o dos tipos de cromosomas con satélite, mientras que la sect. Arachis tiene seis tipos. Un par de cromosomas pequeños se encuentran en la sect. Arachis, excepto en A.batizocoi, A. ipaensis, A. glandulifera, A. magna y A. benensis. A. glandulifera (sect. Arachis tiene el cariotipo más asimétrico, con seis pares de cromosomas subtelocéntricos y A.guaranitica (sect. Trierectoides tiene el cariotipo más simétrico. Esto apoya la hipótesis de que la sect. Trierectoides es la más antigua en el género y la sect. Arachis tiene un origen más reciente. Teniendo en cuenta los cariotipos, morfología externa y la distribución geográfica, A. duranensis y A. ipaensis son probablemente los donantes del genoma de A.hypogaea.

  7. Phylogenetic Relationships in Genus Arachis Based on SSR and AFLP Markers

    Institute of Scientific and Technical Information of China (English)

    TANG Rong-hua; ZHUANG Wei-jian; GAO Guo-qing; HE Liang-qiong; HAN Zhu-qiang; SHAN Shi-hua; JIANG Jing; LI Yang-rui

    2008-01-01

    Fourteen wild species of different sections in the genus Arachis and 24 accessions of the AABB allotetraploid A. hypogaea (cultivated peanut) from several countries which belong to different botanical varieties, were analyzed by SSR and AFLP marker systems. The assay-units per system needed to distinguish among all the tested accessions were at least five for SSR or two for AFLP. The genetic distance detected by the SSR markers ranged from 0.09 to 0.95, and the mean was 0.73; and the genetic distance detected by the AFLP markers ranged from 0.01 to 0.79 with an average of 0.42. All the tested peanut SSR primer pairs were multilocus ones, and the amplified fragments per SSR marker in each peanut genome ranged from 2 to 15 with the mean of 4.77. The peanut cultivars were closely related to each other, and shared a large numbers of SSR and AFLP fragments. In contrast, the species in the genus Arachis shared few fragments. The results indicated that the cultivated peanut (A. hypogaea L.) varieties could be partitioned into two main groups and four subgroups at the molecular level, and that A. duranensis is one of the wild ancestors of A. hypogaea. The lowest genetic variation was detected between A. cardenasii and A. batizocoi, and the highest was detected between A. pintoi and the species in the section Arachis. The relationships among the botanical varieties in the cultivated peanut (A. hypogaea L.) and among wild species accessions in section Arachis and those in other sections in the genus Arachis were discussed.

  8. Spermidine and Flavonoid conjugates from Peanut (Arachis hypogaea) Flower

    Science.gov (United States)

    A new triamide has been isolated from peanut flowers and identified as di-p-(EE)-coumaroylacetylspermidine on the basis of detailed analysis of NMR, MS, and UV data. Two other spermidine conjugates, N1, N5, N10-tri-p-(EEE)-coumaroylspermidine and di-p-(EE)-coumaroylspermidine, as well as four flavon...

  9. Induced variability in quantitative characters of groundnut (Arachis hypogaea L.)

    OpenAIRE

    N. G. Sonone, S. N. Deshmukh, S. B. Lanjewar and R. L. Bhakre

    2010-01-01

    Groundnut genotype AK-280 was treated with EMS 40 mM, 60 mM, gamma rays 30 kR, 40 kR, 50 kR and combination treatment of EMS 20mM + gamma rays 20 kR. The observations on various quantitative characters were recorded in M2 generation. The flowering and maturityperiod was not much affected. For the character height of main axis genotype showed differential response to different treatments of mutagens.Significantly reduced height was observed in 40 kR gamma rays (7.57 cm) as compared to control....

  10. Induced variability in quantitative characters of groundnut (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    N. G. Sonone, S. N. Deshmukh, S. B. Lanjewar and R. L. Bhakre

    2010-12-01

    Full Text Available Groundnut genotype AK-280 was treated with EMS 40 mM, 60 mM, gamma rays 30 kR, 40 kR, 50 kR and combination treatment of EMS 20mM + gamma rays 20 kR. The observations on various quantitative characters were recorded in M2 generation. The flowering and maturityperiod was not much affected. For the character height of main axis genotype showed differential response to different treatments of mutagens.Significantly reduced height was observed in 40 kR gamma rays (7.57 cm as compared to control. Lower doses of EMS (40 mM and combinedtreatment of EMS 20 mM + gamma rays 20 kR proved effective in increasing the number of primary branches. Significant increase in number ofsecondary branches was observed in various treatments. Significant reduction in number of hanging pegs per plant was observed in 40 kRtreatment. Number of matured pods per plant were found decreased in all the treatments except 40 mM EMS treatment. Pod yield per plant andkernel yield per plant were found increased in combined treatment of EMS 20 mM + gamma rays 20 kR. Hundred pod weight and hundred kernelweight was found increased in combined treatments and in lower dose of gamma rays (30 kR. Combined treatments and lower doses ofmutagens were found effective in increasing sound mature kernel per cent. Shelling per cent and oil content were found increased in all thetreatments. In the present investigation the combined treatment of EMS 20 mM + gamma rays 20 kR and gamma rays 40 kR alone were foundmore effective for inducing wide range of mutation in groundnut.

  11. CORRELATION AND PATH COEFFICIENT ANALYSIS IN GROUNDNUT (ARACHIS HYPOGAEA L.)

    OpenAIRE

    C. Pavan Kumar; R Rekha; O. Venkateswarulu; R P Vasanthi

    2014-01-01

    Sixty six genotypes of groundnut were used to study the correlation and path analysis for yield and yield contributing characters. Correlation studies revealed that kernel yield was significantly and positively associated with pod yield per plant, number of mature pods per plant, shelling percentage, harvest index, sound mature kernel percentage, specific leaf weight at 60 DAS, protein content and oil content. Path coefficient analysis indicated that pod yield per plant and shelling percentag...

  12. Genetic divergence in Virginia groundnut (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    R. P. Gupta, J. H. Vachhani, V. H. Kachhadia, M. A. Vaddoria and V. J. Bhatiya

    2015-06-01

    Full Text Available Divergence analysis among sixty groundnut genotypes was carried out using Mahalanobis’s D2-statistic. The genotypes were grouped into thirteen clusters. The maximum inter-cluster distance (D=36.51 was found between clusters III and V followed by clusters IV and V (D=32.67 and II and IV (D=24.21 indicated that these groups of genotypes were highly divergent from each other. The genotypes in above clusters revealed substantial difference in the means for important yield contributing characters suggesting that the genotypes belonging to these clusters from ideal parents for yield improvement in groundnut.

  13. Mutagenic response of peanut (arachis hypogaea L.) to fast neutrons

    International Nuclear Information System (INIS)

    Dominant seeds of peanut were treated with varying doses of fast neutrons employing efficient pre- and post-irradiation techniques for determination of various plant responses useful in a long range program of mutation breeding. Mean reductions in seedling height and frequency of M1 somatic mutations increased with increasing radiation dose. No reduction in M1 seedset was obtained even in the dose above the LD50 of 1600 rads. The frequency of M2 macromutations ranged from only 3.60 - 5.65 per 100 M2 plants. The genetic basis of each of these radiation responses is briefly discussed. The high sensitivity of M1 peanut seedlings and growing plants to fast neutrons is probably due to the highly differentiated seed embryo at the time of irradiation while the radioresistance exhibited by the matured plant appears to be related to the polyploid genome of the species. (Auth.)

  14. Genetic Divergence Analysis in Groundnut (Arachis hypogaea L.)

    OpenAIRE

    Yaikhom Vivekananda, Pramesh Khoyumthem and N. Brajendra Singh

    2015-01-01

    Genetic diversity among 31 genotypes of groundnut were estimated using Mahalanobis D2 statistic for five agro-morphological characters. The analysis of variance revealed significant differences among the genotypes for all characters. Based on Tocher's method, 31 genotypes were grouped into seven clusters, where cluster I was the largest containing 11 genotypes followed by cluster II and III with 7 genotypes each. The inter-cluster distance was maximum between cluster I and cluster VI followed...

  15. CORRELATION AND PATH COEFFICIENT ANALYSIS IN GROUNDNUT (ARACHIS HYPOGAEA L.

    Directory of Open Access Journals (Sweden)

    C. Pavan Kumar

    2014-02-01

    Full Text Available Sixty six genotypes of groundnut were used to study the correlation and path analysis for yield and yield contributing characters. Correlation studies revealed that kernel yield was significantly and positively associated with pod yield per plant, number of mature pods per plant, shelling percentage, harvest index, sound mature kernel percentage, specific leaf weight at 60 DAS, protein content and oil content. Path coefficient analysis indicated that pod yield per plant and shelling percentage had high positive direct effect on kernel yield signifying the importance of these traits in the improvement of seed yield.

  16. Mutation breeding of groundnuts (Arachis hypogaea L.) in Uganda

    International Nuclear Information System (INIS)

    In 1976, mutation breeding of groundnuts was carried out by irradiating the seeds of the Red Beauty (B1) variety with 1500 rad of fast neutrons or 20 krad of 60Co gamma rays. After initial selection, eight promising mutant lines were selected for further testing. In 1989, the mutants were evaluated at three locations and compared with their parent variety. The results obtained showed that seven of the mutant lines gave higher average yields than the parent variety. 2 refs, 1 tab

  17. Eficiência reprodutiva no amendoim cultivado (Arachis hypogaea L. Reproductive efficiency in the peanut (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    Cândida H. T. Mendes Conagin

    1960-01-01

    Full Text Available Cinco variedades de amendoim, abrangendo os três tipos vegetativos conhecidos, foram comparadas quanto à sua eficiência reprodutiva. Para conhecer o resultado final de porcentagem de frutos e sementes, foram estudadas a quantidade de flôres, a duração e as oscilações do florescimento; a formação de frutos e sementes também foi estudada com detalhes. Tendo variado o número de plantas observadas nos diversos itens, em diversos anos, tôdas as informações foram referidas a "uma planta" para poderem ser comparadas quando necessário. O período útil de florescimento dura as dez semanas medianas do ciclo vegetativo da planta, o máximo de flôres sendo atingido na 10.ª semana dêsse ciclo. Há, entretanto, a observar, que as variedades eretas são mais precoces do que as de porte decumbente; o florescimento apresenta bruscas oscilações diárias. É a variedade Tatuí a que produz mais flôres por planta, tendo, entretanto, a mais baixa eficiência reprodutiva. As outras variedades têm eficiência mais alta, mas, mesmo assim, ela é surpreendentemente baixa, em se tratando de uma planta de grande florescimento como é o amendoim. As informações obtidas nestes ensaios são fundamentais para qualquer trabalho de melhoramento por hibridação e já estão sendo utilizadas nos atuais cruzamentos, feitos pela Seção de Citologia.A study of the reproductive efficiency of five agronomical varieties of peanut was made. Determinations were made of fruit and seed percentages in relation to number of flowers procured, length of flowering period, alternation of high and low daily flower frequencies, and number of flowers which had no chance to become fruit?. Data obtained supported lhe following conclusions: (a Varieties of the erect vegetative type were early; they began and ended their vegetative am! flowering cycles before those of the decumbent type. (b The flowering curves showed an abrupt alternation of high and low frequencies. (c The highest flower frequency occurred on the same day for the five varieties tinder test. (d The erect varieties produced well located flowers (the writer calls them useful flowers during a few weeks. For the decumbent varieties this period was longer. (e Fruit percentages in relation to total flowering varied from 15.9% to 25.6%. Calculated in relation to the useful flowers these values varied from 25.4% to 51.3%. The seed percentages were from 12.7% to 21.5% in the first case, and from 21.7% to 38.4% in the second. (f The var. Tatuí and Roxo gave the best fruit percentages in relation to the number of useful flowers. Tatu and B-33 gave the highest seed percentages.

  18. TAXONOMIA DEL GENERO ARACHIS (LEGUMINOSAE Taxonomy of the genus Arachis (Leguminosae

    Directory of Open Access Journals (Sweden)

    Antonio Krapovickas

    2010-11-01

    Full Text Available

    Pasaron casi 100 años entre la designación por Linneo de la entonces única especie de Arachis (A. hypogaea L. conocida por los europeos, y el primer tratamiento taxonómico del
    género por Bentham en 1841. Durante los siguientes 100 años, aparecieron cinco a diez descripciones de especies adicionales, que asignaban diferentes especies a los mismos nombres, y diferentes nombres a las mismas especies. A mediados del Siglo XX, era imposible
    examinar un ejemplar de herbario de Arachis y asignar con alguna certeza algún epíteto a algún espécimen (que no fuera un ejemplar tipo excepto a A. hypogaea, A. guaranitica, A. tuberosa y
    A. villosulicarpa.
    En nuestro tratamiento, la literatura de este caos botánico en Arachis esta revisada en detalle y
    se hace un análisis de los fundamentos de su ocurrencia. Se demuestra que las bases de la
    confusión moran en la combinación de la naturaleza esotérica de los caracteres morfológicos diferenciados de Arachis, de los especímenes fragmentarios de antaño, y de la representación
    de especies por plántulas.
    Además, se relata cómo, en 1959, decidimos reexplorar la localidad tipo de cada especie hasta
    entonces conocida, y recolectar allí especímenes de las plantas enteras y así resolver el problema. Después de treinta y cinco años, dos generaciones de coleccionistas de plantas, y
    alrededor de 2000 colecciones, presentamos aquí las descripciones de 69 especies de Arachis,
    especies distribuidas en Sudamérica al este de los Andes, al sud del Amazonas, al norte de La
    Plata y desde el noroeste argentino hasta el nordeste de Brasil.
    Descubrimos muy pronto que los caracteres más significativos de Arachis residen en sus estructuras subterráneas, incluyendo sus frutos, tallos rizomatosos, sistemas radicales e

  19. Draft genome of the peanut A-genome progenitor (Arachis duranensis) provides insights into geocarpy, oil biosynthesis, and allergens.

    Science.gov (United States)

    Chen, Xiaoping; Li, Hongjie; Pandey, Manish K; Yang, Qingli; Wang, Xiyin; Garg, Vanika; Li, Haifen; Chi, Xiaoyuan; Doddamani, Dadakhalandar; Hong, Yanbin; Upadhyaya, Hari; Guo, Hui; Khan, Aamir W; Zhu, Fanghe; Zhang, Xiaoyan; Pan, Lijuan; Pierce, Gary J; Zhou, Guiyuan; Krishnamohan, Katta A V S; Chen, Mingna; Zhong, Ni; Agarwal, Gaurav; Li, Shuanzhu; Chitikineni, Annapurna; Zhang, Guo-Qiang; Sharma, Shivali; Chen, Na; Liu, Haiyan; Janila, Pasupuleti; Li, Shaoxiong; Wang, Min; Wang, Tong; Sun, Jie; Li, Xingyu; Li, Chunyan; Wang, Mian; Yu, Lina; Wen, Shijie; Singh, Sube; Yang, Zhen; Zhao, Jinming; Zhang, Chushu; Yu, Yue; Bi, Jie; Zhang, Xiaojun; Liu, Zhong-Jian; Paterson, Andrew H; Wang, Shuping; Liang, Xuanqiang; Varshney, Rajeev K; Yu, Shanlin

    2016-06-14

    Peanut or groundnut (Arachis hypogaea L.), a legume of South American origin, has high seed oil content (45-56%) and is a staple crop in semiarid tropical and subtropical regions, partially because of drought tolerance conferred by its geocarpic reproductive strategy. We present a draft genome of the peanut A-genome progenitor, Arachis duranensis, and 50,324 protein-coding gene models. Patterns of gene duplication suggest the peanut lineage has been affected by at least three polyploidizations since the origin of eudicots. Resequencing of synthetic Arachis tetraploids reveals extensive gene conversion in only three seed-to-seed generations since their formation by human hands, indicating that this process begins virtually immediately following polyploid formation. Expansion of some specific gene families suggests roles in the unusual subterranean fructification of Arachis For example, the S1Fa-like transcription factor family has 126 Arachis members, in contrast to no more than five members in other examined plant species, and is more highly expressed in roots and etiolated seedlings than green leaves. The A. duranensis genome provides a major source of candidate genes for fructification, oil biosynthesis, and allergens, expanding knowledge of understudied areas of plant biology and human health impacts of plants, informing peanut genetic improvement and aiding deeper sequencing of Arachis diversity. PMID:27247390

  20. Draft genome of the peanut A-genome progenitor (Arachis duranensis) provides insights into geocarpy, oil biosynthesis, and allergens

    Science.gov (United States)

    Chen, Xiaoping; Li, Hongjie; Pandey, Manish K.; Yang, Qingli; Wang, Xiyin; Garg, Vanika; Li, Haifen; Chi, Xiaoyuan; Doddamani, Dadakhalandar; Hong, Yanbin; Upadhyaya, Hari; Guo, Hui; Khan, Aamir W.; Zhu, Fanghe; Zhang, Xiaoyan; Pan, Lijuan; Pierce, Gary J.; Zhou, Guiyuan; Krishnamohan, Katta A. V. S.; Chen, Mingna; Zhong, Ni; Agarwal, Gaurav; Li, Shuanzhu; Chitikineni, Annapurna; Zhang, Guo-Qiang; Sharma, Shivali; Chen, Na; Liu, Haiyan; Janila, Pasupuleti; Li, Shaoxiong; Wang, Min; Wang, Tong; Sun, Jie; Li, Xingyu; Li, Chunyan; Wang, Mian; Yu, Lina; Wen, Shijie; Singh, Sube; Yang, Zhen; Zhao, Jinming; Zhang, Chushu; Yu, Yue; Bi, Jie; Zhang, Xiaojun; Paterson, Andrew H.; Wang, Shuping; Liang, Xuanqiang; Varshney, Rajeev K.; Yu, Shanlin

    2016-01-01

    Peanut or groundnut (Arachis hypogaea L.), a legume of South American origin, has high seed oil content (45–56%) and is a staple crop in semiarid tropical and subtropical regions, partially because of drought tolerance conferred by its geocarpic reproductive strategy. We present a draft genome of the peanut A-genome progenitor, Arachis duranensis, and 50,324 protein-coding gene models. Patterns of gene duplication suggest the peanut lineage has been affected by at least three polyploidizations since the origin of eudicots. Resequencing of synthetic Arachis tetraploids reveals extensive gene conversion in only three seed-to-seed generations since their formation by human hands, indicating that this process begins virtually immediately following polyploid formation. Expansion of some specific gene families suggests roles in the unusual subterranean fructification of Arachis. For example, the S1Fa-like transcription factor family has 126 Arachis members, in contrast to no more than five members in other examined plant species, and is more highly expressed in roots and etiolated seedlings than green leaves. The A. duranensis genome provides a major source of candidate genes for fructification, oil biosynthesis, and allergens, expanding knowledge of understudied areas of plant biology and human health impacts of plants, informing peanut genetic improvement and aiding deeper sequencing of Arachis diversity. PMID:27247390

  1. TAXONOMY OF THE GENUS ARACHIS (LEGUMINOSAE Taxonomy of the genus Arachis (Leguminosae

    Directory of Open Access Journals (Sweden)

    Antonio Krapovickas

    2010-08-01

    Full Text Available

    Pasaron casi 100 años entre la designación por Linneo de la entonces única especie de Arachis (A. hypogaea L. conocida por los europeos, y el primer tratamiento taxonómico del
    género por Bentham en 1841. Durante los siguientes 100 años, aparecieron cinco a diez
    descripciones de especies adicionales, que asignaban diferentes especies a los mismos
    nombres, y diferentes nombres a las mismas especies. A mediados del Siglo XX, era imposible
    examinar un ejemplar de herbario de Arachis y asignar con alguna certeza algún epíteto a algún
    espécimen (que no fuera un ejemplar tipo excepto a A. hypogaea, A. guaranitica, A. tuberosa y
    A. villosulicarpa.
    En nuestro tratamiento, la literatura de este caos botánico en Arachis esta revisada en detalle y
    se hace un análisis de los fundamentos de su ocurrencia. Se demuestra que las bases de la
    confusión moran en la combinación de la naturaleza esotérica de los caracteres morfológicos
    diferenciados de Arachis, de los especímenes fragmentarios de antaño, y de la representación
    de especies por plántulas.
    Además, se relata cómo, en 1959, decidimos reexplorar la localidad tipo de cada especie hasta
    entonces conocida, y recolectar allí especímenes de las plantas enteras y así resolver el
    problema. Después de treinta y cinco años, dos generaciones de coleccionistas de plantas, y
    alrededor de 2000 colecciones, presentamos aquí las descripciones de 69 especies de Arachis,
    especies distribuidas en Sudamérica al este de los Andes, al sud del Amazonas, al norte de La
    Plata y desde el noroeste argentino hasta el nordeste de Brasil.
    Descubrimos muy pronto que los caracteres más significativos de Arachis residen en sus
    estructuras subterráneas, incluyendo sus frutos, tallos rizomatosos, sistemas radicales e

  2. Uji Efektivitas Fungisida Sistemik Dan Non Sistemik Terhadap Perkembangan Penyakit Karat (Puccinia arachidis. Speg) Pada Tanaman Kacang Tanah (Arachis hypogeae. L)

    OpenAIRE

    Nelwati

    2012-01-01

    Penelitian ini bertujuan untuk mengetahui pengaruh fungisida sistemik dan non-sistemik terhadap perkembangan penyakit karat daun ( Puccinia arachidis. Speg ) pada tanaman kacang tanah (Arachis hypogaea. L). Penelitian ini dilaksanakan di Tanjung Sari kecamatan Medan Selayang dengan ketinggian ± 25 m diatas permukaan laut. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) non faktorial tediri dari 5 ulangan 5 perlakuan FO kontrol, F1 fungisida Score 250 EC dengan dosis 1,87 ml/l a...

  3. Taxonomy of the genus Arachis (Leguminosae by Antonio Krapovickas and Walton C. Gregory

    Directory of Open Access Journals (Sweden)

    Antonio Krapovickas

    2007-01-01

    Full Text Available Almost 100 years elapsed between Linnaeus’ naming the then lone species of Arachis (A.hypogaea L. known to Europeans, and the first taxonomic treatment of the genus by Bentham in1841. During the next 100 years five to ten additional species descriptions appeared, assigningdifferent species to the same names, and different names to the same species. By mid-20thCentury, it was impossible to examine any herbarium collection of Arachis and assign any epithetwith any assurance to any specimen (which was not a type collection except to A. hypogaea, A.guaranitica, A. tuberosa and A. villosulicarpa.In our treatment, the literature of this botanical chaos in Arachis is reviewed in detail and anassessment is made of the foundations for its occurrence. It is shown that the bases for theconfusion lay in the combination of the esoteric nature of the differentiating morphologicalfeatures of Arachis, the fragmentary early collections, and the representation of species byseedling specimens.Also, it is related how, in 1959, we decided to re-explore the type locality of each species thenknown, collect therein complete plant specimens and thereby resolve the problem. Thirty-fiveyears, two generations of plant collectors and around 2000 collections later, we present here 69species descriptions of Arachis, species distributed in South America east of the Andes, south ofthe Amazon, north of La Plata and from NW Argentina to NE Brazil.We soon discovered that the most significant characters of Arachis lay in their undergroundstructures, including their fruits, rhizomatous stems, root systems and hypocotyls.We showed that these defining characters tended to cluster the collections into groups whichwere associated with generally different geographic areas and ecological features.We drew a sample of 100 collections representing these clusters, areas and features, andarranged them in a hybridization diallel and showed, in crosses between collections representingdifferent

  4. Effect of cooking and roasting on the amino acid composition of raw groundnut (Arachis hypogaea seeds

    Directory of Open Access Journals (Sweden)

    Emmanuel Ilesanmi Adeyeye

    2010-06-01

    Full Text Available Background. As a food, groundnut is one of the most concentrated products, since it is very high in calories due to its high fat and protein content. One gram of groundnut supplies 6.03 kcal (25.2 kJ compared with 4 kcal (16.7 kJ for pure cane sugar, 3.6 kcal (15.1 kJ for polished rice, 3.5 kcal (14.6 kJ for maize flour and 2.7 kcal (11.3 kJ. Groundnut flour is used as food and 2.7 kcal (11.3 kJ. Groundnut flour is used as food in soup or stew, sauces, confectionaries, puddings, bakery products and in various other ways for human consumption. These uses of groundnut necessitate the determination of the general composition of groundnut. Material and methods. About 1.0 kg dried seeds of groundnut was involved in the analysis of the sample. It was divided into three parts for the dry, dry cooked and dry roasted samples. Defatted groundnut seeds were used using standard analytical method in which chloroform/methanol (2:1 v/v was used as extraction solvent. Ion exchange chromatography method was used in the analysis of the amino acid composition using the Technicon Sequential Multisample (TSM Amino Acid Analyser. Various methods were used to determine the quality parameters of the protein. Other parameters were the predicted protein efficiency ratio (P-PER, essential amino acid index (EAAI and the isoelectric point (pI. The statistical analysis was also carried out. Results. Total amino acid was as follows: (g/100 g crude protein, cp: 83.5 (raw seeds, Rs, 85.9 (cooked seeds, Cs and 66.8 (roasted seeds, Rt.s with corresponding essential amino acids as: 39.4 or 47.2% (Rs, 38.3 or 44.6% (Cs and 30.0 or 44.9% (Rt.s. Predicted protein efficiency ratios were 2.55 (Rs, 3.00 (Cs and 2.31 (Rt.s and essential amino acid index of 1.18 (Rs, 1.08 (Cs and 0.83 (Rt.s. Cooking enhanced the amino acid levels of Asp, Ser, Glu, Pro, Arg, Ala, Cys, Val, Leu and Phe. The following essential amino acids were reduced by both cooking and roasting: Lys (15.9-27.6%, His (4.23- -16.5%, Thr (40.1-60.6%, Met (38.0-63.4% and Ile (13.3-31.8%. All the parameters between Rs/Cs and most of the parameters between Rs/Rt.s were significantly different at r=0.05.

  5. Effect of plant growth regulators and drought stress on groundnut (arachis hypogaea L.) genotypes

    International Nuclear Information System (INIS)

    Experiments were carried out to investigate the physiological (dry weight of root, stem, peg, flowers plant/sup -1/ fruits sets percent, pod yield (kg plot /sup -1/, 100-kernel weight, days to flowering and maturity) and biochemical (endogenous proline level ) traits of groundnut cultivar Swat Phalli-96 under drought stress. The result showed that drought stress significantly (p less than or equal to 0.05) reduced dry weight of root, peg/flowers plant/sup -1/, fruits sets percent, pod yield (kg plot/sup -1/), 100-kernel weight, days to 0.05) effect on various parameters under drought stress conditions. Foliar application of ABA (10/sup - 4/ M) partially reduced the adverse effect of drought stress on growth and yield components. Foliar application of ABA to plants when subsequently exposed to drought stress resulted in elevated levels of endogenous shoot and root proline levels. (author)

  6. Response of groundnut (arachis hypogaea L.) genotypes to plant growth regulators and drought stress

    International Nuclear Information System (INIS)

    The present study was carried out to investigate the physiological (relative water content) pod dry weight, pods plant/sup -1/, pod yield (kg plot/sup -1/), shelling (%), plant height and biochemical (endogenous ABA level) traits of peanut cultivar Swat Phalli-96 under drought stress. The result showed that drought stress significantly (p0.05) effect on various parameters under drought stress conditions. However, foliar application of ABA (10/sup -4/ M) partially ameliorated the adverse effects of drought stress on growth and yield components. Foliar application of ABA to plants when subsequently exposed to drought stress resulted in elevated levels of endogenous ABA. The endogenous ABA levels in shoot increased earlier in response to applied ABA than that of root. (author)

  7. Allergens of Arachis hypogaea and the effect of processing on their detection by ELISA

    Directory of Open Access Journals (Sweden)

    Amjad Iqbal

    2016-02-01

    Full Text Available Food allergies are an emerging public health problem in industrialized areas of the world. They represent a considerable health problem in these areas because of the relatively high number of reported cases. Usually, food allergens are proteins or glycoproteins with a molecular mass ranging from 10 to 70 kDa. Among the food allergies, peanut is accounted to be responsible for more than 50% of the food allergy fatalities. Threshold doses for peanut allergenic reactions have been found to range from as low as 100 µg to 1 g of peanut protein, which equal to 400 µg to 4 g peanut meal. Allergens from peanut are mainly seed storage proteins that are composed of conglutin, vicilin, and glycinin families. Several peanut proteins have been identified to induce allergic reactions, particularly Ara h 1–11. This review is mainly focused on different classes of peanut allergens, the effect of thermal and chemical treatment of peanut allergens on the IgY binding and detectability of these allergens by enzyme linked immunosorbent assay (ELISA to provide knowledge for food industry.

  8. Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes.

    Science.gov (United States)

    Liu, Hongzhi; Jiang, Nan; Liu, Li; Sheng, Xiaojing; Shi, Aimin; Hu, Hui; Yang, Ying; Wang, Qiang

    2016-01-01

    The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X₁), extraction time (X₂) and ratio of water to raw material (X₃). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 10⁵ Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide. PMID:27258246

  9. Ground Arthropod Attacks on Groundnut Arachis hypogaea L in Burkina Faso

    OpenAIRE

    Dicko, IO.; Troaoré, S.; Traoré, D.; Dao, B.

    1999-01-01

    Studies were conducted in five districts of Burkina Faso, West Africa from November to December, 1996. The objectives aimed at establishing spatial distribution and quantifying the level of damages on peanut pods by soil arthropods, termites and millepedes. Twenty seven samples of 100 pods each were taken from farmers' stocks in each district, which made a total of 135 pod samples examined. Damage was determined in each district by counting scarified pods by termites and perforated pods by mi...

  10. Effects of Drought and Heat on Peanut (Arachis hypogaea, L.) Production

    OpenAIRE

    Balota, Maria

    2012-01-01

    Discusses effects of drought and heat on peanut plants and crop yield, and offers advice on if and when to irrigate fields, and for drought-tolerant varieties of peanuts to use in fields with a history of drought.

  11. DNA fingerprinting of groundnut (Arachis hypogaea L.) varieties of Tirupati using SSR markers

    OpenAIRE

    Y. Amaravathi, R.P. Vasanthi, E. Siva Kumar, M. Purushotham and T. Giridhara Krishna

    2014-01-01

    Unambiguous identification of varieties is important for registration and certification of newly released varieties. Molecular markers are powerful tools, which help in differentiating plant varieties at the DNA level and have been widely used for fingerprinting in a number of crop varieties. In the present study, a set of 12 groundnut varieties released from Regional Agricultural Research Station, Tirupati were fingerprinted employing SSR markers. A total of 300 SSR were screened and fifteen...

  12. DNA fingerprinting of groundnut (Arachis hypogaea L. varieties of Tirupati using SSR markers

    Directory of Open Access Journals (Sweden)

    Y. Amaravathi, R.P. Vasanthi, E. Siva Kumar, M. Purushotham and T. Giridhara Krishna

    2014-12-01

    Full Text Available Unambiguous identification of varieties is important for registration and certification of newly released varieties. Molecular markers are powerful tools, which help in differentiating plant varieties at the DNA level and have been widely used for fingerprinting in a number of crop varieties. In the present study, a set of 12 groundnut varieties released from Regional Agricultural Research Station, Tirupati were fingerprinted employing SSR markers. A total of 300 SSR were screened and fifteen potential markers were employed for fingerprinting of groundnut varieties. The SSR markers generated alleles ranging from 2 to 7 with an average of four per locus. The polymorphism information content (PIC values ranged from 0 to 0.85. The genotypic data from all the loci provided unique SSR allelic fingerprints which helped in varietal identification of groundnut. Core set of highly informative primers viz., PM 377, TC1A02, TC5A06 and GM1489 identified in this study has the potential to identify most of the groundnut varieties. Cluster analysis using SSR marker grouped 12 groundnut varieties into two major clusters. Finger printing of the groundnut genotypes provide information about phylogenetic relationships and assists groundnut breeders in varietal registration and protection of intellectual property rights.

  13. Response of groundnut (Arachis hypogaea L. genotypes to soil fertilization of micronutrients in alfisol conditions

    Directory of Open Access Journals (Sweden)

    P. Arunachalam , P. Kannan, J. Prabhaharan, G. Prabukumar and Zadda Kavitha

    2013-03-01

    Full Text Available In this study, an attempt was made to assess the genetic variability of kernel micronutrients and to enrich the micronutrientcontent in groundnut. Twenty one different genotypes and three cultivars were evaluated for micronutrient response underalfisol condition. To enhance the soil micronutrient availability recommended dose of Fe and Zn were applied as basal. Theexperiment was conducted at Dryland Agricultural Research Station, Chettinad under rainfed condition during August toDecember 2011. Yield parameters and micronutrients content in kernel were assessed. In alfisol, basal application of Zn andFe enhanced the soil available Zn and Fe content. Basal application of micronutrients increased the kernel Zn content to anaverage of 28.7 per cent and pod yield of 12.6 per cent in groundnut cultivars. Significant genetic variability was observedamong genotypes for Fe, Zn, Cu and Mn enrichment in kernel. The accessions ICGV 07225, ICGV 07222, ICGV 07247and ICGV 07220 were having inherent ability to load high zinc content in kernel with high pod yield per plant. Fordeveloping high yielding zinc dense cultivars, the genotype identified in this study are to be tested in diverse environmentsfor their stability

  14. Rancang Bangun Alat Pengupas Kulit Ari Kacang Tanah (Arachis hypogaea) Tipe Engkol

    OpenAIRE

    Agus Sutejo; Adithya Rakhmat Prayoga

    2012-01-01

    One cause of reduced productivity of peanut husk is peeled peeling process is still done manually, using the power of man. To overcome this, a system designed to cuticle peeling peanuts which facilitates mechanical stripping process peanut husk. Peeling epidermis is mechanically done by using two rubber-covered rollers are designed to be able to peel the peanut husk easily. Having conducted research, produced peeler bean husk, which consists of, Hopper, stringer system, the framework, dirt th...

  15. Management of Stem-rot of Groundnut (Arachis hypogaea L. Cultivar in Field

    Directory of Open Access Journals (Sweden)

    Khirood DOLEY

    2013-08-01

    Full Text Available The present experiment was conducted at University of Pune for biocontrol of soil-borne plant pathogen Sclerotium rolfsii by incorporating arbuscular mycorrhizal fungi (Glomus fasciculatum and conventional system of cultivation with different spacing pattern (15 and 30 cm in field. Both mycorrhizal inoculation and 30 cm spacing pattern significantly increased growth and yield as compared to control or 15 cm spacing pattern. The pathogenic mycorrhizal groundnut plants in 30 as well as 15 cm spacing pattern showed better growth in terms of plant height, leaf and pod number, fresh and dry weight of whole groundnut plant in comparison to non-mycorrhizal pathogenic ones and the plant growth was better in 30 spacing than 15 cm. The colonization by AM fungi in both spacing pattern was higher in absence of pathogen S. rolfsii. However, pathogen’s presence decreased the mycorrhizal colonization considerably in 30 and 15 cm. The disease severity and incidence were recorded to be lowered when inoculated with mycorrhiza in pathogenic groundnut plants as compared to non-mycorrhizal pathogenic ones in both spacing pattern and incidence and severity was significantly lower in 30 cm as compared to 15 cm. Therefore, it was observed from our results that for management of soil-borne pathogens inoculation of AM fungi and spacing patterns are necessary.

  16. Gamma ray Induced Light-orange Flower Mutant in Groundnut (Arachis hypogaea L. and its inheritance

    Directory of Open Access Journals (Sweden)

    A. Mothilal* and M. Jayaramachandran

    2012-03-01

    Full Text Available A dwarf mutant plant having small leaves and light-orange flower was identified in the M2 generation from gammaray treated groundnut cultivar VRI 2. The mutant bred true in successive generations. An investigation was made toknow the genetics of light-orange flower in groundnut mutant. The Spanish bunch cultivar VRI 2 having orangeflower was crossed with the light-orange flower mutant. The F1 plants exhibited orange flower indicating that theorange colour is governed by dominant gene. However, the F2 population segregated as 3 orange: 1 light-orangeflower indicating the light-orange flower trait is governed by a single recessive gene.

  17. Shotgun label-free quantitative proteomics of developing peanut (Arachis hypogaea L.) seed

    Science.gov (United States)

    Legume seeds and peanuts, in particular, are an inexpensive source of plant proteins and edible oil. Owing to their importance in global food security, it is necessary to understand the genetic, biochemical, and physiological mechanisms controlling seed quality and nutritive attributes. A comprehens...

  18. Regulation of the Phenylpropanoid Pathway: A Mechanism of Selenium Tolerance in Peanut (Arachis hypogaea L.) Seedlings.

    Science.gov (United States)

    Wang, Guang; Wu, Liying; Zhang, Hong; Wu, Wenjia; Zhang, Mengmeng; Li, Xiaofeng; Wu, Hui

    2016-05-11

    To clarify the mechanisms of selenium (Se) tolerance in peanut seedlings, we grew peanut seedlings with sodium selenite (0, 3, and 6 mg/L), and investigated the phenylpropanoids metabolism in seedling roots. The results showed that selenite up-regulated the expression of genes and related enzyme activities involving in the phenylpropanoids biosynthesis cascade, such as phenylalanine ammonia-lyase, trans-cinnamate-4-hydroxylase, chalcone synthase, chalcone isomerase, and cinnamyl-alcohol dehydrogenase. Selenite significantly increased phenolic acids and flavonoids, which contributed to the alleviation of selenite-induced stress. Moreover, selenite enhanced the formation of endodermis in roots, which may be attributed to the up-regulation of lignin biosynthesis mediated by the selenite-induced changes of H2O2 and NO, which probably regulated the selenite uptake from an external medium. Accumulation of polyphenolic compounds via the phenylpropanoid pathway may be one of the mechanisms of the increasing selenite tolerance in plants, by which peanut seedlings survived in seleniferous soil, accompanied by accumulation of Se. PMID:27089243

  19. Heterosis and inbreeding depression for pod yield and its components in groundnut (Arachis hypogaea L..

    Directory of Open Access Journals (Sweden)

    H.K. Gor , L.K. Dhaduk and Lata Raval

    2012-09-01

    Full Text Available Thirty-six crosses generated in a diallel fashion excluding reciprocals using nine genotypes were evaluated to study the extent of heterosis and inbreeding depression for pod yield and yield attributing characters in groundnut. Considerable heterosis over better parent (heterobeltiosis was observed for the number of mature pods per plant, harvest index and biological yield, while the traits like pod and kernel yield per plant showed low heterobeltiosis, and it was absent or in negative direction for the traits like days to 50% flowering, days to maturity, 100-kernel weight, shelling outturn and sound mature kernels in all the crosses. High inbreeding depression was recorded for days to maturity and 100-kernel weight, while fully matured kernels and biological yield did not show any inbreeding depression. The cross combination, TPG 41 × AK 303 exhibited significant and positive heterobeltiosis (16.4%, highest standard heterosis (123.1% and maximum mean performance (24.2g for pod yield per plant. Only one cross (NRCG 115 × AK 303 out of 36, showed significant and high inbreeding depression for pod yield per plant. Negative estimates of heterotic effects observed in some traits may be attributed to inter-allelic interactions. No clear-cut relationship between heterosis and inbreeding depression was observed for pod yield and related traits. Superior segregants from the cross, TPG 41 × AK 303 may be selected for further improvement in the pod yield and its contributing traits.

  20. Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea Cakes

    Directory of Open Access Journals (Sweden)

    Hongzhi Liu

    2016-06-01

    Full Text Available The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC were investigated in this study. A Box-Behnken factorial design (BBD was used to investigate the effects of three independent variables, namely extraction temperature (X1, extraction time (X2 and ratio of water to raw material (X3. The optimum conditions were 85 °C, 3 h and 20:1 (mL/g respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 105 Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.

  1. Identification and characterization of a gene encoding a putative lysophosphatidyl acyltransferase from Arachis hypogaea

    Indian Academy of Sciences (India)

    Si-Long Chen; Jia-Quan Huang; Lei Yong; Yue-Ting Zhang; Xiao-Ping Ren; Yu-Ning Chen; Hui-Fang Jiang; Li-Ying Yan; Yu-Rong Li; Bo-Shou Liao

    2012-12-01

    Lysophosphatidyl acyltransferase (LPAT) is the important enzyme responsible for the acylation of lysophosphatidic acid (LPA), leading to the generation of phosphatidic acid (PA) in plant. Its encoding gene is an essential candidate for oil crops to improve oil composition and increase seed oil content through genetic engineering. In this study, a full-length AhLPAT4 gene was isolated via cDNA library screening and rapid amplification of cDNA ends (RACE); our data demonstrated that AhLPAT4 had 1631 nucleotides, encoding a putative 43.8 kDa protein with 383 amino acid residues. The deduced protein included a conserved acyltransferase domain and four motifs (I–IV) with putative LPA and acyl-CoA catalytic and binding sites. Bioinformatic analysis indicated that AhLPAT4 contained four transmembrane domains (TMDs), localized to the endoplasmic reticulum (ER) membrane; detailed analysis indicated that motif I and motifs II–III in AhLPAT4 were separated by the third TMD, which located on cytosolic and ER luminal side respectively, and hydrophobic residues on the surface of AhLPAT4 protein fold to form a hydrophobic tunnel to accommodate the acyl chain. Subcellular localization analysis confirmed that AhLPAT4 was a cytoplasm protein. Phylogenetic analysis revealed that AhLPAT4 had a high homology (63.7–78.3%) with putative LPAT4 proteins from Glycine max, Arabidopsis thaliana and Ricinus communis. AhLPAT4 was ubiquitously expressed in diverse tissues except in flower, which is almost undetectable. The expression analysis in different developmental stages in peanut seeds indicated that AhLPAT4 did not coincide with oil accumulation.

  2. Nutritional composition and antioxidant activity of Spanish and Virginia groundnuts (Arachis hypogaea L.): a comparative study.

    Science.gov (United States)

    Mahatma, M K; Thawait, L K; Bishi, S K; Khatediya, N; Rathnakumar, A L; Lalwani, H B; Misra, J B

    2016-05-01

    Kernels of sixty groundnut genotypes comprising thirty each of Spanish and Virginia groups were characterized and compared for the content of oil, protein, phenols and antioxidant activity along with their fatty acid and sugars profiles. The antioxidant activity for Virginia genotypes was ranged from 12.5 to 16.5 μM Trolox equivalent activity for Spanish genotypes ranged from 6.8-15.2 μM. Amongst Virginia types, the highest oleic acid/linoleic acid (O/L) ratio of 2.38 was observed for NRCG 12312 while from Spanish group the highest O/L ratio of 1.24 was observed for NRCG 12731. The sucrose content for Virginia genotypes ranged from 38.5 to 69.0 mg/g while it was 27.9 to 53.3 mg/g for Spanish genotypes. Average myo-inositol content was higher for Spanish genotypes (0.8-2.1 mg/g) compared to Virginia (0.4-1.8 mg/g) while the reverse was true for stachayose content (Spanish: 3.5-7.9 mg/g; Virginia: 4.6-10.3 mg/g). Thus, Virginia genotypes could be preferred to Spanish genotypes for better oil stability and antioxidant activity. PMID:27407194

  3. Molecular Detection of Aflatoxin Producing Strains of Aspergillus Flavus from Peanut (Arachis Hypogaea

    Directory of Open Access Journals (Sweden)

    Adeela Hussain

    2015-02-01

    Full Text Available Aflatoxins are the potential carcinogens produced as secondary metabolites by Aspergillus flavus. They have the ability to contaminate large number of food which ultimately affect the human population. Malt extract agar was selected for the growth of control stains of fungus. The aim of the study was to develop a reliable and quick method for the detection of aflatoxin producing strains in peanuts by using molecular approaches. Total 80 samples of infected peanuts were collected from four different cities of Punjab and checked for their aflatoxin contamination. For aflatoxin detection, three target genes nor1, ver1 and aflR were selected which was involved in the aflatoxin biosynthesis. In all examined cases, 24 out of 80 (30% samples successfully amplified all three genes indicating aflatoxigenic activity. Discrimination between aflatoxigenic and non-aflatoxigenic strains were also determined on the basis of amplification of these three target DNA fragments. In this study, it was also demonstrated that only specific strains were able to produce the aflatoxin contamination in peanuts.

  4. Rancang Bangun Alat Pengupas Kulit Ari Kacang Tanah (Arachis hypogaea Tipe Engkol

    Directory of Open Access Journals (Sweden)

    Agus Sutejo

    2012-10-01

    Full Text Available One cause of reduced productivity of peanut husk is peeled peeling process is still done manually, using the power of man. To overcome this, a system designed to cuticle peeling peanuts which facilitates mechanical stripping process peanut husk. Peeling epidermis is mechanically done by using two rubber-covered rollers are designed to be able to peel the peanut husk easily. Having conducted research, produced peeler bean husk, which consists of, Hopper, stringer system, the framework, dirt thrower fan / epidermis, and hoppers expenses. From the test results from test 10 times, each repetition is about 100 grams paring the results obtained about 70% whole shelled peanuts. Or can be calculated with engine capacity of about 35 kg / hr with a percentage split of about 35%, it is because the rubber on the roll is less balanced / less flashlight, so the workmanship is required with appropriate accuracy by using a lathe.

  5. Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.

    Indian Academy of Sciences (India)

    Meng-Jun Li; Ai-Qin Li; Han Xia; Chuan-Zhi Zhao; Chang-Sheng Li; Shu-Bo Wan; Yu-Ping Bi; Xing-Jun Wang

    2009-06-01

    The cultivated peanut is a valuable source of dietary oil and ranks fifth among the world oil crops. Plant fatty acid biosynthesis is catalysed by type II fatty acid synthase (FAS) in plastids and mitochondria. By constructing a full-length cDNA library derived from immature peanut seeds and homology-based cloning, candidate genes of acyl carrier protein (ACP), malonyl-CoA:ACP transacylase, -ketoacyl-ACP synthase (I, II, III), -ketoacyl-ACP reductase, -hydroxyacyl-ACP dehydrase and enoyl-ACP reductase were isolated. Sequence alignments revealed that primary structures of type II FAS enzymes were highly conserved in higher plants and the catalytic residues were strictly conserved in Escherichia coli and higher plants. Homologue numbers of each type II FAS gene expressing in developing peanut seeds varied from 1 in KASII, KASIII and HD to 5 in ENR. The number of single-nucleotide polymorphisms (SNPs) was quite different in each gene. Peanut type II FAS genes were predicted to target plastids except ACP2 and ACP3. The results suggested that peanut may contain two type II FAS systems in plastids and mitochondria. The type II FAS enzymes in higher plants may have similar functions as those in E. coli.

  6. Genotypic Differences for Reproductive Growth, Yield, and Yield Components in Groundnut (Arachis hypogaea L.)

    OpenAIRE

    ÇALIŞKAN, Sevgi; ÇALIŞKAN, Mehmet Emin; Arslan, Mehmet

    2008-01-01

    This study was conducted to evaluate reproductive growth, oil and protein accumulation in seeds, and some yield components of 8 groundnut genotypes in 2001 and 2002. The number of flowers, pegs, and pods per plant during the reproductive period were determined with periodic counts starting from flowering. The percentage of flowers turned to pegs and pods, and the percentage of pegs turned to pods were calculated at the end of the growing period. After pod setting, 6 plants per plot were harve...

  7. Isolation and expression analysis of LEA genes in peanut (Arachis hypogaea L.)

    Indian Academy of Sciences (India)

    Lei Su; Chuan-Zhi Zhao; Yu-Ping Bi; Shu-Bo Wan; Han Xia; Xing-Jun Wang

    2011-06-01

    Late embryogenesis abundant (LEA) protein family is a large protein family that includes proteins accumulated at late stages of seed development or in vegetative tissues in response to drought, salinity, cold stress and exogenous application of abscisic acid. In order to isolate peanut genes, an expressed sequence tag (EST) sequencing project was carried out using a peanut seed cDNA library. From 6258 ESTs, 19 LEA-encoding genes were identified and could be classified into eight distinct groups. Expression of these genes in seeds at different developmental stages and in various peanut tissues was analysed by semi-quantitative RT-PCR. The results showed that expression levels of LEA genes were generally high in seeds. Some LEA protein genes were expressed at a high level in non-seed tissues such as root, stem, leaf, flower and gynophore. These results provided valuable information for the functional and regulatory studies on peanut LEA genes.

  8. Bradyrhizobium vignae sp. nov., a nitrogen-fixing symbiont isolated from effective nodules of Vigna and Arachis.

    Science.gov (United States)

    Grönemeyer, Jann Lasse; Hurek, Thomas; Bünger, Wiebke; Reinhold-Hurek, Barbara

    2016-01-01

    Twenty one strains of symbiotic bacteria from root nodules of local races of cowpea (Vigna unguiculata), Bambara groundnut (Vigna subterranea) and peanuts (Arachis hypogaea) grown on subsistence farmers' fields in the Kavango region of Namibia, were previously characterized as a novel group within the genus Bradyrhizobium. To verify their taxonomic position, the strains were further analysed using a polyphasic approach. 16S rRNA gene sequences were most similar to Bradyrhizobium manausense BR 3351T, with Bradyrhizobium ganzhouense RITF806T being the most closely related type strain in the phylogenetic analysis, and Bradyrhizobium yuanmingense CCBAU 10071T in the ITS sequence analysis. Phylogenetic analysis of concatenated glnII-recA-rpoB-dnaK placed the strains in a highly supported lineage distinct from species of the genus Bradyrhizobium with validly published names; they were most closely related to Bradyrhizobium subterraneum 58 2-1T. The status of the species was validated by results of DNA-DNA hybridization. The combination of phenotypic characteristics from several tests, including carbon source utilization and antibiotic resistance, could be used to differentiate representative strains of species of the genus Bradyrhizobium with validly published names. Novel strain 7-2T induced effective nodules on Vigna subterranea, Vigna unguiculata, Arachis hypogaea and on Lablab purpureus. The DNA G+C content of strain 7-2T was 65.4 mol% (Tm). Based on the data presented, we conclude that these strains represent a novel species for which the name Bradyrhizobium vignae sp. nov. is proposed, with strain 7-2T [LMG 28791T, DSMZ 100297T, NTCCM0018T (Windhoek)] as the type strain. PMID:26463703

  9. Advances in Arachis through genomics and biotechnology

    Science.gov (United States)

    The 5th International Conference of the peanut research community met in Brasilia, Brazil from June 13 through 16, 2011 to discuss “Advances in Arachis through genomics and biotechnology”. Over 100 participated from many countries such as United States, Japan, China, India, Brazil, Argentina, with ...

  10. Efeito de herbicidas no desenvolvimento e na concentração de nutrientes em amendoim (Arachis Hypogaea L. I. Herbicidas incorporados ao solo Effect of herbicides on growth and nutrient content in Arachis Hypogaea L. I. Soil incorporated herbicides

    Directory of Open Access Journals (Sweden)

    R. Detjbert

    1978-01-01

    Full Text Available Foram conduzidos dois experimentos de campo, um em solo areno-barrento e outro em solo argiloso, com a finalidade de estudar o efeito de trifluralin, aplicado nas doses de 0,60 e 0,96.kg/ha e vernolate, nas doses de 2,52 e 3,60 kg/ha, incorporados ao solo, sobre o desenvolvimento de amendoim e teor de macronutrientes em folhas no início do florescimento e sementes na colheita, e o teor de óleo nestas. O desenvolvimento da cultura foi observado por meio de pesos de matéria seca de folhas, caules e vagens, durante o ciclo, obtendo -se, também, a produção de vagens no fim do ciclo. Houve pequena redução no desenvolvimento, nos estágios iniciais do ciclo, pelas doses mais elevadas de trifluralin e vernolate em solo argiloso apenas. Os teores de N, P, K, Ca, Mg e S, não foram afetados em folhas nem nos grãos por nenhum tratamento, nos dois experimentos, assim como o teor de óleo. As produções de grãos em casca não foram afetadas por nenhum tratamento, sendo elevadas nos dois experimentos.The effect of triflurali n, at 0,60 and 0,92 kg/ha, and vernolate at 2,52 and 3,60 kg/ha, soil incorporated on growth and macronutrients content of groundnuts, was studied in two field trials, on sandy -loam and clay soils. Nutr ient content was determined in the fourth leave at flowering and in seeds, at harvest, when also oil content was analysed. The crop growth was observed by means of dry matter weight of leaves, stems and pods, during the cycle, and yield measured at its end. There was a slight reduction in growth, during the first half part of the cycle, when the higher rates of trifluralin and vernola te was applied on clay soil. The contents of N, P, K, Ca, Mg and S were no affected on leaves or seeds b y any treatment in both experiments as to oil. The yield was not affected either, by any treatment.

  11. Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi, a wild peanut.

    Science.gov (United States)

    Singh, Naveen Kumar; Kumar, Koppolu Raja Rajesh; Kumar, Dilip; Shukla, Pawan; Kirti, P B

    2013-01-01

    Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants. PMID:24367621

  12. Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi, a wild peanut.

    Directory of Open Access Journals (Sweden)

    Naveen Kumar Singh

    Full Text Available Peanut (Arachis hypogaea L is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants.

  13. 木薯、野花生的他感作用%Allelopathy of Manihot utilissima and Arachis pintoi

    Institute of Scientific and Technical Information of China (English)

    朱朝华; 骆焱平; 许敏; 颜桂军

    2006-01-01

    用木薯(Manihot utilissima Crantz.)茎叶的汁液及根系土壤分别处理南瓜(Cucurbita moschata Duch.)、玉米(Zea mays L)、豇豆(Vigna sinensis)、花生(Arachis hypogaea)、热研2号柱花草(Stylosanthes guiaensis cv.Reyan No.2)、热研8号坚尼草(Panicum maximum cv.Reyan No.8);用野花生(Arachis pintoi)茎叶汁液及根系土壤分别处理胜红蓟(Ageratum conyzoides L)、小飞蓬[Comnyza canadensis(L.)Cronq.]、粘人草(Bidens pilosa L.),测定其对种子萌发和生长的影响.结果表明:木薯茎叶的汁液对南瓜、玉米、豇豆、花生、热研2号柱花草、热研8号坚尼草的种子萌发和生长均有影响;木薯根系土壤对南瓜、玉米、豇豆、花生的种子萌发影响均不明显,对南瓜、玉米、豇豆、花生的露白种子生长均有影响.野花生茎叶的汁液对胜红蓟的种子萌发有影响,对胜红蓟、小飞蓬和粘人草的生长均有影响;野花生根系土壤对胜红蓟和小飞蓬的种子萌发影响均不明显,对胜红蓟、小飞蓬和粘人草的生长均有影响.

  14. Interaction Effects of Insecticides on Enzyme Activities in Black Clay Soil from Groundnut (Arachis hypogaea L. Fields

    Directory of Open Access Journals (Sweden)

    C. Nasreen

    2012-06-01

    Full Text Available In practice pesticides are extensively used in agriculture as a part of pest control strategies. Two insecticides, endosulfan (organochlorine and profenophos (organophosphate, were assessed for their effects on the activities of protease (in terms of tyrosine formed from casein and urease (as ammonia released from urea in soil, collected from a fallow groundnut field by applications of insecticides at normal field rates and at higher concentrations (1.0, 2.5, 5.0, 7.5, 10.0 kg ha-1, in a laboratory study. The results showed a strong positive influence on protease and urease enzyme activities in soil treated with 2.5 and 5.0 kg ha-1 dry soil and they were significantly (P ≤ 0.05 higher than the control over the course of incubation. In soil treatment, there was a significant increase in protease and decrease in urease activities after 24h of incubation which continued up to 20 days. However, a significant decrease in both protease and urease enzyme activities was observed in 30 and 40 days of incubation.DOI: http://dx.doi.org/10.5755/j01.erem.60.2.671

  15. INHERITANCE STUDIES OF PLANT HEIGHT, POD AND SEED ATTRIBUTES IN F2 GENERTION OF CERTAIN GROUNDNUT (ARACHIS HYPOGAEA L. CROSSES

    Directory of Open Access Journals (Sweden)

    Sudha Jyothi Dandu

    2013-03-01

    Full Text Available Inheritance of plant height, pod length, pod width, seed length and seed width was studied in a set of crosses grown during rabi 2007-08. F2 phenotypic data for plant height was a good fit to a phenotypic ratio of 1:4:6:4:1 and 1:2:1 respectively in two crosses studied indicating the involvement of more than two genes. Segregation of pod length in F2 generation of the cross ICGV 00350 x K 1340, was a good fit to a phenotypic ratio of 45 medium short : 19 long pods while in the cross ICGV 00350 x TG 37A segregation of pod length was a good fit to a phenotypic ratio of 1 long : 2 medium : 1 short pod length. Segregation of pod width in the crosses ICGV 00350 x K 1340 and ICGV 00350 x TG 37A presented a good fit to a phenotypic ratio of 3 high width: 1 medium width pods, while in the cross ICGV 00350 x Jyothi, F2 segregation data was a good fit to a phenotypic ratio of 1 high width: 2 medium width: 1 low width pods. F2 segregation of seed length in the cross ICGV 00350 x K 1340 was a good fit to a phenotypic ratio of 13 long medium: 3 short. Segregation of seed width in F2 generation of the crosses K 1340 x TCGS 888 and K 1340 x Narayani was a good fit to a phenotypic ratio of 15 medium low : 1 high width and 3 low : 1 medium width seeds.

  16. Caracterización y clasificación de Germoplasma Mexicano de Cacahuate (Arachis hypogaea L.)

    OpenAIRE

    Samuel Sánchez Domínguez; Abel Muñoz Orozco; Victor Arturo González Hernández; Ángel Martínez Garza

    2006-01-01

    Debido a la gran variación de ambientes en México que inducen una considerable interacción genotipo-ambiente, y con objeto de generar información básica para futuras investigaciones, se estudiaron 64 colecciones y variedades de cacahuate que se sembraron en el verano de 1988 en dos localidades del Estado de Morelos: Cuauchichinola (buen ambiente, S0) y Miacatlán (ambiente limitante, S1). Se midieron 33 características con las que se hizo análisis de varianza y de agrupamientos. Número de frut...

  17. Growth analysis and yield of two varieties of groundnut (Arachis hypogaea L.) as influenced by different weed control methods

    OpenAIRE

    Olayinka, Bolaji U.; Etejere, Emmanuel O.

    2015-01-01

    Field trials were carried out to evaluate the effects of seven weed management strategies on the growth and yield of two groundnut varieties (Samnut 10 and MK 373) for two successive seasons (2010–2011). The experimental layout was a split plot complete randomized block design with three replications. The two groundnut varieties showed identical pattern of results for leaf area index, dry matter accumulation, relative growth rate, net assimilation rate and crop growth rate as well as yield. A...

  18. Screening of a peanut (Arachis hypogaea L.) cDNA library to isolate a Bowman-Birk trypsin inhibitor clone.

    Science.gov (United States)

    Boateng, Judith A; Viquez, Olga M; Konan, Koffi N; Dodo, Hortense W

    2005-03-23

    Peanut crop losses due to insect and pest infestation cost peanut farmers nearly 20% of their annual yields. The conventional use of chemicals to combat this problem is costly and toxic to humans and livestock and leads to the development of resistance by target insects. Transgenic plants expressing a trypsin inhibitor gene in tobacco and cowpea have proven to be efficient for resistance against insects. Therefore, a transgenic peanut overexpressing a trypsin inhibitor gene could be an alternative solution to the use of toxic chemicals. Five Bowman-Birk trypsin inhibitor (BBTI) proteins were previously isolated from peanut. However, to date, neither cDNA nor genomic DNA sequences are available. The objective of this research was to screen a peanut cDNA library to isolate and sequence at least one full-length peanut BBTI cDNA clone. Two heterologous oligonucleotides were constructed on the basis of a garden pea (Pisum sativa) trypsin inhibitor nucleotide sequence and used as probes to screen a peanut lambda gt-11 cDNA library. Two positive and identical cDNA clones were isolated, subcloned into a pBluescript vector, and sequenced. Sequence analysis revealed a full-length BBTI cDNA of about 243 bp, with a start codon ATG at position +1 and a stop codon TGA at position +243. In the 3' end, two poly adenylation signals (AATAAA) were identified at positions +261 and +269. The isolated cDNA clone encodes a protein of 80 amino acid residues including a leader sequence of 11 amino acids. The deduced amino acid sequence is 100% identical to published sequences of peanut BBTI AI, AII, BI, and BIII and 81% identical to BII. PMID:15769131

  19. Induction of mutations for plant height and inheritance of dwarf mutant in groundnut (Arachis hypogaea L. through gamma ray irradiation

    Directory of Open Access Journals (Sweden)

    Anand M. Badigannavar and Suvendu Mondal

    2010-03-01

    Full Text Available Gamma ray induced mutagenesis of groundnut cultitvar TAG 24 evolved true breeding several mutantsaffecting various morphological traits. Among them, 16 mutants were dwarf and three were tall. Plant heightwas reduced by 24.5% to 41.0% in dwarf mutants and increased by 13.1 to 30.6% in tall mutants. Progeniesfrom an interesting dwarf mutant consistently segregated into dwarf, extreme dwarf and parental types. From thehybridization between mutant and its parent, it was concluded that dwarf mutation was due to monogenicincomplete dominance.

  20. Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (Arachis hypogaea L)

    OpenAIRE

    Li Ling; Chen Xiaoping; Zhang Erhua; Wang Tong; Liang Xuanqiang

    2010-01-01

    Abstract Background Pre-harvest infection of peanuts by Aspergillus flavus and subsequent aflatoxin contamination is one of the food safety factors that most severely impair peanut productivity and human and animal health, especially in arid and semi-arid tropical areas. Some peanut cultivars with natural pre-harvest resistance to aflatoxin contamination have been identified through field screening. However, little is known about the resistance mechanism, which has slowed the incorporation of...

  1. Identification of seed proteins associated with resistance to pre-harvested aflatoxin contamination in peanut (Arachis hypogaea L

    Directory of Open Access Journals (Sweden)

    Li Ling

    2010-11-01

    Full Text Available Abstract Background Pre-harvest infection of peanuts by Aspergillus flavus and subsequent aflatoxin contamination is one of the food safety factors that most severely impair peanut productivity and human and animal health, especially in arid and semi-arid tropical areas. Some peanut cultivars with natural pre-harvest resistance to aflatoxin contamination have been identified through field screening. However, little is known about the resistance mechanism, which has slowed the incorporation of resistance into cultivars with commercially acceptable genetic background. Therefore, it is necessary to identify resistance-associated proteins, and then to recognize candidate resistance genes potentially underlying the resistance mechanism. Results The objective of this study was to identify resistance-associated proteins in response to A. flavus infection under drought stress using two-dimensional electrophoresis with mass spectrometry. To identify proteins involved in the resistance to pre-harvest aflatoxin contamination, we compared the differential expression profiles of seed proteins between a resistant cultivar (YJ-1 and a susceptible cultivar (Yueyou 7 under well-watered condition, drought stress, and A. flavus infection with drought stress. A total of 29 spots showed differential expression between resistant and susceptible cultivars in response to A. flavus attack under drought stress. Among these spots, 12 protein spots that consistently exhibited an altered expression were screened by Image Master 5.0 software and successfully identified by MALDI-TOF MS. Five protein spots, including Oso7g0179400, PII protein, CDK1, Oxalate oxidase, SAP domain-containing protein, were uniquely expressed in the resistant cultivar. Six protein spots including low molecular weight heat shock protein precursor, RIO kinase, L-ascorbate peroxidase, iso-Ara h3, 50 S ribosomal protein L22 and putative 30 S ribosomal S9 were significantly up-regulated in the resistant cultivar challenged by A. flavus under drought stress. A significant decrease or down regulation of trypsin inhibitor caused by A. flavus in the resistant cultivar was also observed. In addition, variations in protein expression patterns for resistant and susceptible cultivars were further validated by real time RT-PCR analysis. Conclusion In summary, this study provides new insights into understanding of the molecular mechanism of resistance to pre-harvest aflatoxin contamination in peanut, and will help to develop peanut varieties with resistance to pre-harvested aflatoxin contamination.

  2. Characterization of B-1, 3-glucanase gene in peanut (Arachis hypogaea L.) by cloning and genetic transformation

    Science.gov (United States)

    Plant ß-1,3-glucanase is commonly found to be involved in the disease resistance. A ß-1,3-glucanase gene was isolated from both the genomic DNA and cDNA of peanut variety Huayu20 by PCR and RT-PCR, respectively (GenBank Accession No. JQ801335). The genomic DNA sequence was 1,471 bp including two ext...

  3. Investigations into the agronomic and economic aspects of using peanut (Arachis hypogaea) as an on-farm biodiesel feedstock

    Science.gov (United States)

    Although farmers have benefited from the creation of transportation fuels from grain and oilseeds, little research has addressed single farm or community self-reliance on home-grown fuels. The Peanut Biodiesel Project is designed to determine if peanut is suitable for just such a concept through fi...

  4. Multilocation trial of potential selected mutant lines of groundnut (arachis hypogaea) at 3 location in Peninsular Malaysia

    International Nuclear Information System (INIS)

    Two fixed mutant lines of groundnut derived from cultivar Matjan were selected for their yield potential at M10 generation. Multilocation trial of these mutants (MJ40/42 and MJ20/165-5) was carried out to evaluate genotype stability at different climate and soil types in Peninsular Malaysia. The mutant lines were planted and compared with their parent (Matjan) and control variety (MKT1). The identified locations were in Taiping (Perak), Machang (Kelantan), and Air Hitam (Johor). The soils at the locations were of the Serdang, Bungor and Rengam series, respectively. The trial was carried out simultaneously in the same year at each location. Mutant MJ20/165-5 showed stable performance at all location compared to other genotypes tested. Its yield was higher than the parent in Kelantan and Johor trial and showed similar performance in Perak. This mutant also showed better yield performance than the control varieties in the Kelantan trial. Meanwhile, mutant line MJ40/42 gave better yield in Kelantan and Johor but did not perform well in Perak as compared to its parent and control varieties. (Author)

  5. AN OVERVIEW ON ROLE OF PHOSPHORUS AND WATER DEFICITS ON GROWTH, YIELD AND QUALITY OF GROUNDNUT (ARACHIS HYPOGAEA L.

    Directory of Open Access Journals (Sweden)

    S. Hemalatha

    2013-08-01

    Full Text Available Fertilizer application in crop plants normally result in enhanced crop yields up to certain levels. Each of these major and minor nutrients have specific role in producing growth and yield enhancement in agricultural crops. However, the exact time and dose of fertilizer application in the given crop schedule is also of paramount importance for successfully exploiting the crop’s potentiality in terms of yield. Groundnut is an important oilseed crop in the tropics and semi-arid tropics. Yield and often quality of oil in groundnut is sizeably dependent on proper application of different nutrients that have a direct say on these attributes. Phosphorus application in groundnut has tremendous impact on growth and development in groundnut. Further, the effect of phosphorus on yield and yield attributes is also well established. Another important factor determining crop growth and yields in groundnut is water. During periods of unpredictable water shortages, within season adjustments of water scheduling must be made in relation to the difference in the yield sensitivity to water deficits on groundnut and its individual growth periods. Thus management of limited water supplies for increased crop production requires studies on water production function of groundnut. This paper reviews information on phosphorus fertilization studies in groundnut and also in identifying moisture sensitive periods in groundnut for limited water management.

  6. An electrospray ionisation-mass spectrometry screening of triacylglycerols in developing cultivated and wild peanut kernels (Arachis hypogaea L.).

    Science.gov (United States)

    Cherif, Aicha O; Leveque, Nathalie; Ben Messaouda, Mhamed; Kallel, Habib; Moussa, Fathi

    2013-06-01

    The accumulation of triacylglycerols during the development of three varieties of peanuts was monitored in two Tunisian cultivated peanut (Trabelsia (AraT) and Chounfakhi (AraC)) and one wild Tunisian peanut (Arbi (AraA)). The presence of TAGs composed of rare fatty acid residues such as hexacosanoic acid (C(23:0)) and heneicosanoic acid (C(21:0)) among the triacylglycerols C(23:0) LL, C(23:0) OO and C(21:0) LL was noted. The major molecular species of triacylglycerol detected in the three peanut varieties were dioleoyl linoleoyl (OOL), 1,2,3-trioleyl (OOO), 1,2-dioleyl-3-palmitoyl (POO), 1,2-dilinoleoyl-3-oleyl (OLL) and 1-oleoyl-2-linoleoyl-3-linolenoyl (OLLn). The TAG composition and content were significantly different among the three peanut varieties. The three major TAGs were OOL (20.6%), OOO (15.6%) and OLLn (13.2%) in AraA; OOL (21.4%), OOO (20.1%) and POO (17.5%) in AraC and finally OLL (20.7%), OOO (19.8%) and OLL (17.7%) in AraT. PMID:23411219

  7. In Vitro Inhibition of Cellulolytic Enzymes of Fusarium Oxysporum by Trichoderma spp and Pseudomonas Fluorescens on Arachis Hypogaea L

    OpenAIRE

    P.Rajeswari

    2015-01-01

    In an attempt to develop biocontrol system for management of Fusarium wilt in groundnut, Trichoderma viride, Trichoderma harzianum,and Pseudomonas fluorescens were evaluated for their antagonistic activity against Fusarium oxysporum in vitro. .Fusarium wilt diseasescaused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtratesof T.viride (1%), T. harzianum (1.5%), and P. fluorescens (2%) on the in vitro inhibiti...

  8. Physiological responses of peanut (Arachis hypogaea L.) cultivars to water deficit stress: status of oxidative stress and antioxidant enzyme activities

    OpenAIRE

    Chakraborty, Koushik; Singh, Amrit L.; Kalariya, Kuldeep A.; Goswami, Nisha

    2015-01-01

    From a field experiment, the changes in oxidative stress and antioxidant enzyme activities was studied in six Spanish peanut cultivars subjected to water deficit stress at two different stages viz. pegging and pod development stages. Imposition of water deficit stress significantly reduced relative water content, membrane stability and total carotenoid content in all the cultivars, whereas total chlorophyll content increased at initially and decreased at later stage. Chlorophyll a/b ratio inc...

  9. Pendugaan Faktor-Faktor Produksi Kacang Tanah (Arachis hypogaea L) Pada Lahan Sawah di Kecamatan Tanjung Morawa Kabupaten Deli Serdang

    OpenAIRE

    Sihombing, Mayesti D.

    2012-01-01

    This study was conducted in Medan Senembah village, Pardamean village, and Telaga Sari village, Tanjung Morawa district Deli Serdang regency. The observation had been begin since October 2004 up to July 2005, was taken by auger to represent each area of the studied land. The sample was taken at the depth of 0 - 25 cm. Furtheymore calculate with multiple regression method and factor analysis used SPSS program. Prediction is first step to conclude land suitability. This observation porpose f...

  10. Pertumbuhan dan Produksi Kacang Tanah (Arachis hypogaea L.) Dengan Pemberian Kompos Tandan Kosong Kelapa Sawit Pada Frekuensi Pembumbunan Yang Berbeda

    OpenAIRE

    Sembiring, Michael

    2015-01-01

    The purpose of this study was to determine the effect of empty fruit bunch compost and pile up frequency toward the growth and production of peanut. This research was held on Jalan Penampungan, Delitua, Namorambe, Deli Serdang with altitude ± 25 metre,took place on April to July 2013 . The study design was a factorial randomized block design with two factors, namely empty palm bunches (0, 12, 24 and 36 g /plant) and pile up frequency (0; 21; 21,42 and 21,42,63 days). The parameters measured ...

  11. Effects of leaf movement on radiation interception in field grown leguminous crops, 1: Peanut (Arachis hypogaea L.)

    International Nuclear Information System (INIS)

    The effects of leaf movement of peanut on radiation interception were examined. A peanut cultivar (c.v. Nakateyutaka) was planted at three planting densities (20, 30 and 40 cm equidistant spacings). In the treatment plots, the upper layer of the canopy was covered horizontally with a nylon net to restrain the movement of the leaflets. Intercepted radiation of each leaflet was measured by integrated solarimeter films for two consecutive days. It was observed that the leaflets of the upper layer oriented paraheliotropically to the sun rays in midday. Intercepted radiation per unit leaf area and unit ground area of the control were larger in the 20 cm pacing, almost similar in the 30 cm spacing and smaller in the 40 cm spacing as compared with the treatment. The leaf movement of the upper layer of the canopy played a significant role in radiation interception in the 20 cm plot, no discernible effect in the 30 cm plot and a rather adverse role in the 40 cm plot. Leaf area of the 20 cm spacing was concentrated densely at the upper layer. Leaf area of the 30 and 40 cm spacing was larger at the middle layers. It was assumed that effectiveness of the leaf movement of the upper layer would depend mainly on spatial leaf area distribution and density

  12. Assessment of competition for water between peanut (Arachis hypogaea) and Choerospondias axillaris in an alley cropping system in subtropical China

    International Nuclear Information System (INIS)

    Tree and crop components of an alley cropping system may compete for nutrients and water in the surface soil while the deeper-rooting tree may have better access to resources in the subsoil. The objectives of the present study in an alley cropping system were to monitor runoff and soil loss, spatial variation of soil water, and tree sap flow to determine competitiveness for water between the tree and peanut. Plant and root growth were also recorded to evaluate competition. The tree competed for water in the surface soil at some critical period as indicated by the spatial variation of monitored soil water content. The tree used deep soil water and reduced deep drainage as indicated by modelling of soil water fluxes and direction of water movement. Trees in the alley cropping system used less soil water than peanut, and more water than trees alone, as indicated by water balance modelling. The magnitude of the reduction of deep drainage increased with tree age. The alley cropping system increased system water use efficiency by using deep soil water as indicated by water balance modelling. The alley cropping system promoted Choerospondias axillaris growth by 50 to 100% and reduced peanut biomass and yield by 20 to 50%. Field management should aim to prevent water competition between trees and crops in the surface soil. (author)

  13. Cryopreservation of Arachis pintoi (leguminosae) somatic embryos.

    Science.gov (United States)

    Rey, H Y; Faloci, M; Medina, R; Dolce, N; Engelmann, F; Mroginski, L

    2013-01-01

    In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1 degree C per min from 25 degree C to -30 degree C followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30 degree C water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles. PMID:24441368

  14. Species relations among wild Arachis species with the A genome as revealed by FISH mapping of rDNA loci and heterochromatin detection.

    Science.gov (United States)

    Robledo, G; Lavia, G I; Seijo, G

    2009-05-01

    Section Arachis of the homonymous genus includes 29 wild diploid species and two allotetraploids (A. monticola and the domesticated peanut, A. hypogaea L.). Although, three different genomes (A, B and D) have been proposed for diploid species with x = 10, they are still not well characterized. Moreover, neither the relationships among species within each genome group nor between diploids and tetraploids (AABB) are completely resolved. To tackle these issues, particularly within the A genome, in this study the rRNA genes (5S and 18S-26S) and heterochromatic bands were physically mapped using fluorescent in situ hybridization (FISH) in 13 species of Arachis. These molecular cytogenetic landmarks have allowed individual identification of a set of chromosomes and were used to construct detailed FISH-based karyotypes for each species. The bulk of the chromosome markers mapped revealed that, although the A genome species have a common karyotype structure, the species can be arranged in three groups (La Plata River Basin, Chiquitano, and Pantanal) on the basis of the variability observed in the heterochromatin and 18S-26S rRNA loci. Notably, these groups are consistent with the geographical co-distribution of the species. This coincidence is discussed on the basis of the particular reproductive traits of the species such as autogamy and geocarpy. Combined with geographic distribution of the taxa, the cytogenetic data provide evidence that A. duranensis is the most probable A genome ancestor of tetraploid species. It is expected that the groups of diploid species established, and their relation with the cultigen, may aid to rationally select wild species with agronomic traits desirable for peanut breeding programs. PMID:19234686

  15. Taxonomy Icon Data: Peanut [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available gaea_S.png Arachis_hypogaea_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Arachis+hypogaea&t=L http://biosciencedbc.jp/taxo...nomy_icon/icon.cgi?i=Arachis+hypogaea&t=NL http://biosciencedbc.jp/taxonomy_icon/ic...on.cgi?i=Arachis+hypogaea&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Ar...achis+hypogaea&t=NS http://togodb.biosciencedbc.jp/togodb/view/taxonomy_icon_comment_en?species_id=207 ...

  16. Rumen parameters of sheep fed Arachis pintoi cv. Belmonte hay

    Directory of Open Access Journals (Sweden)

    Gisele Machado Fernandes

    2012-12-01

    Full Text Available Studies on animal nutrition have shown factors that affect livestock yield and their interaction with the environment, through assessments on food nutritional values, nutrient metabolism in livestock and biochemical parameters related to nutrition and animal breeding. Inclusion levels of hay Arachis pintoi cv. Belmonte in the diet of sheep were studied by measuring the dry matter intake (DMI, production of short chain fatty acids (SCFA, ammonia nitrogen (NH3-N and rumen pH. Four males Santa Inês sheep with cannulas in the rumen were used in a 4x4 Latin Square design with four periods (21 days each and four treatments that corresponded to the inclusion levels (0%, 30%, 60% and 100% of Arachis pintoi cv. Belmonte to replace grass hay Cynodon dactylon cv. Coastcross in the diet. The DMI showed a quadratic effect increased until treatment with 60% of Arachis and reduced in the treatment with 100% of the legume. Significant interaction was observed between treatments and sampling times for NH3-N and acetate, propionate and butyrate concentration and the acetate:propionate ratio. There was no interaction between treatments and sampling time for rumen pH and total VFA concentration. It is observed a rapid increase in ammonia concentration until 2 hours after the feeding, and then a decline is seen. At 8 hours after the feeding, the quadratic effect occurred (P0.05 increasing NH3-N concentration with 60% of Arachis, declining afterwards. The acetate, propionate and butyrate concentration, showed a quadratic effect (P0.01 in the sampling time after the feeding for treatment with 60% of Arachis with the concentration rising until 4 hours and declining thereafter. For total SCFA concentration, when analyzed individually, we observed the effect of sampling time (P0.05, in which the means showed a quadratic effect, with maximum increase until 4 hours after the feeding, reducing at 8 hours. For acetate:propionate ratio was a linear effect (P0

  17. Tratamentos pré-germinativos em sementes de Arachis pintoi Arachis pintoi seeds as affect by pre-germination

    Directory of Open Access Journals (Sweden)

    Claudia Antonia Vieira Rossetto

    2008-02-01

    Full Text Available Neste Trabalho objetivou-se avaliar o efeito dos tratamentos prévios na germinação e no vigor de sementes de Arachis pintoi Krapov. & W. C. Greg.. O delineamento experimental adotado foi inteiramente casualizado, em esquema fatorial (2 lotes x 7 tratamentos, com quatro repetições. Para isto, foram utilizados dois lotes comerciais de sementes com o pericarpo (frutos de Arachis pintoi, da cv. Amarillo, que estavam armazenados por seis e 12 meses. Por lote, foram empregados os tratamentos de remoção ou não do pericarpo, de quebra do pericarpo, de exposição dos frutos íntegros ao aquecimento a 45º C por 48 e 72 horas e à hidratação por 24 e 48 horas. Posteriormente, por tratamento, foi realizada a avaliação do grau de umidade, da germinação e do vigor (primeira contagem e emergência de plântulas. A remoção do pericarpo tornou as sementes mais vulneráveis à ação dos microrganismos. O aquecimento a 45º C por 48 e 72 horas propiciou a redução das sementes não germinadas. A hidratação por 48 horas favoreceu a germinação e o vigor das sementes de Arachis pintoi.The aim was to evaluate the effects of previous treatments in the Arachis pintoi Krapov. & W. C. Greg. seeds germination and vigour. A completely randomized design with four replication arranged in a factorial scheme (2 lots x 7 treatments, was used. For this, two commercial lots of seeds with intact pods of Arachis pintoi, cv. Amarillo, stored by six and 12 months, were used. In each lot, the treatments were employed through pod removal or not, the pod breakage and the exposition of intact pods at 45ºC for 48 and 72 hours and to the hidratation for 24 and 48 hours. Further, for treatment, it was performed the evaluation of water content, germination and vigor (first count of germination and seedling emergency. The pod removal became the seeds vulnerable to the action of microorganism. Heating at 45ºC for 48 and 72 hours caused reduction of the nom

  18. Ecological aspects of Langsdorffia hypogaea (Balanophoraceae parasitism in the Pantanal wetlands

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    Carlos Rodrigo Lehn

    2015-12-01

    Full Text Available Most studies on holoparasitic plants have focused on taxonomic or systematic issues. The objective of this study was to examine the ecological aspects of parasitism of Langsdorffia hypogaea (Balanophoraceae in the Pantanal wetlands. Individuals of L. hypogaea were dug out by hand and the host trees were identified. Eighty-eight percent of host trees exhibited zoochory dispersal syndrome. Protium heptaphyllum and Cordiera sessilis represented 50% of the sampled trees. Both species are evergreen and are preferentially shade-tolerant species growing under the canopy of other trees. Fecal sample of collared peccaries (Pecari tajacu had seeds from both the host tree (Protium sp. and the parasite L. hypogaea. We therefore propose the hypothesis that P. tajacu play a role as a seed disperser and may affect root parasitism.

  19. Chemical composition of some wild peanut species (Arachis L.) seeds.

    Science.gov (United States)

    Grosso, N R; Nepote, V; Guzmán, C A

    2000-03-01

    Oil, protein, ash, and carbohydrate contents, iodine value, and fatty acid and sterol compositions were studied in seeds of Arachis trinitensis, A. chiquitana, A. kempff-mercadoi, A. diogoi, A. benensis, A. appressipila, A. valida, A. kretschmeri, A. helodes, A. kuhlmannii, A. williamsii, A. sylvestris, A. matiensis, A. pintoi, A. hoehnei, A. villosa, and A. stenosperma. Oil content was greatest in A.stenosperma (mean value = 51.8%). The protein level was higher in A. sylvestris (30.1%) and A. villosa (29.5%). Mean value of oleic acid varied between 30.6% (A. matiensis) and 46.8% (Arachis villosa), and linoleic acid oscillated between 34.1% (A. villosa) and 47.4% (A. appressipila). The better oleic-to-linoleic (O/L) ratio was exhibited by A. villosa (1.38). Some species showed higher concentration of behenic acid. The greatest level of this fatty acid was found in A. matiensis (6.2%). Iodine value was lower in A. valida (99.2). The sterol composition in the different peanut species showed higher concentration of beta-sitosterol (mean values oscillated between 55.7 and 60.2%) followed by campesterol (12.4-16. 5%), stigmasterol (9.7-13.3%), and Delta(5)-avenasterol (9.7-13.4%). The chemical quality and stability of oils (iodine value and O/L ratio) from wild peanut studied in this work are not better than those of cultivated peanut. PMID:10725154

  20. Quantifying N2-fixed by groundnut (Arachis hypogaea L.) as compared to some summer legumes using ''1''5N methodology with different reference crops

    International Nuclear Information System (INIS)

    Using the ''1''5N methodology, one of the cultivar of groundnut repeated once (as groundnut 1 and 2) and one cultivar of each of the summer legumes guar, pigeon pea and mungbean were studied (a) to determine the amounts of nitrogen fixed by these legumes using different reference crops and (b) to compare N-fixation by groundnut to that of the above mentioned summer legumes. The reference crops used were, sorghum, soybean and a non-nodulating groundnut isoline. Each of the studied legumes and reference crops was grown at the Gezira Research Station Farm, in a microplot of 2.4 m''2 situated at one side of a main-plot of 24 m''2. The N2 fixing legumes guar, mung bean, and pigeon pea and sorghum were given 20 kg N/ha as urea at 5.0% ''1''5N atom excess, and the reference crops of soybean and non -nodulating groundnut were given 100 kg N/ha at 1.0% ''1''5N atom excess. ''1''4N/''1''5N ratios were determined in plants sampled from the microplots. The results showed that pigeon pea and guar could compete well with groundnut as N2-fixers. Levels of fixation (%Ndfa) were 79% (108 kg N/ha), 77% (138 kg N/ha) and 80% (70 kg N/ha) of the total crop's N need for guar, groundnut and pigeon pea, respectively. Mungbean fixed about 12% (6 kg N/ha) of its N need. The variation in the amounts of N2 fixed in kg/ha is dependent on the total plant N yield of each legume which was 160-180, 139, 87 and 68 for groundnut, guar, pigeon pea and mug bean, respectively. The non-nodulating groundnut was a superior reference crop over sorghum and soybean. Thus, the studied reference crops can be listed in a descending order of excellence as follows: non-nodulating groundnut, sorghum, soybean.(Author)

  1. Comparing genome guided assembly and phased variants based assembly approach to separate the homoeolog transcripts in tetraploid peanut (Arachis hypogaea L.)

    Science.gov (United States)

    Homoeologous copies of transcripts are abundant in many self-pollinating species including tetraploid peanut, and can impose a challenge to build a transcriptome reference without the merging of homoeologs. De novo transcriptome assembly of tetraploid OLin with single kmer and multiple kmer approach...

  2. Influence of Light Conditions on Biology and Chemistry in the Peanut Plant: Flavonoids and Spermidines from Peanut (Arachis hypogaea) Flowers and Studies of the Photoisomerization of Spermidine Conjugates

    Science.gov (United States)

    Early history and significance of the peanut crop is discussed. Annual world production of peanuts at 30 million tons makes this crop one of the most important agricultural commodities. Unusual physiology, inflorescence, and infructescence of the peanut plant make it an attractive object for scienti...

  3. Composición lipídica de semillas de maní (Arachis hypogaea L. obtenidas bajo diferentes condiciones de disponibilidad de agua

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    Casanoves, Fernando

    2000-12-01

    Full Text Available The changes in the lipid composition, oleic/linoleic ratio (O/L and iodine value (IY for Florman INTA peanut seeds of different sizes obtained under two different water availability conditions during reproductive period were, evaluated. The water availability affected O/L ratio, IY, and the content of all fatty acid but behenic. The seed size affect the oleic, eicosenic and behenic contents. Interaction between water availability and size seed in relation to lipid and lignoseric acid content were also detected. The greatest water availability produced seeds with more lipid content. Moreover, probably due to an indirect effect of less soil temperature, the insaturated lipid concentration was increased as can be seen in the less O/L ratio and augmented IY.Se evaluaron las modificaciones que se producen en el contenido de materia grasa, perfil de ácidos grasos, relación oleico / linoleico (O/L e índice de yodo (IY de las semillas de maní (cv. Florman INTA de distinto tamaño producidas bajo dos situaciones diferentes de disponibilidad de agua durante el período reproductivo. La disponibilidad de agua afectó la relación O/L, el IY, y el contenido de todos los ácidos grasos menos behénico. En relación al tamaño de las semillas variaron el contenido de oleico, eicosenoico y behénico. Se encontró interacción entre disponibilidad hídrica y tamaño de semillas para el contenido de aceite y de ácido lignosérico. La mayor disponibilidad de agua produjo semillas con mayor contenido de aceite y además, probablemente debido a un efecto indirecto de disminución de la temperatura de suelo, se aumentó el grado de insaturación del aceite, lo cual se evidenció a través de una relación O/L menor y un IY superior.

  4. Stress Inducible Expression of AtDREB1A Transcription Factor in Transgenic Peanut (Arachis hypogaea L.) Conferred Tolerance to Soil-Moisture Deficit Stress.

    Science.gov (United States)

    Sarkar, Tanmoy; Thankappan, Radhakrishnan; Kumar, Abhay; Mishra, Gyan P; Dobaria, Jentilal R

    2016-01-01

    Peanut, an important oilseed crop, is gaining priority for the development of drought tolerant genotypes in recent times, since the area under drought is constantly on the rise. To achieve this, one of the important strategies is to genetically engineer the ruling peanut varieties using transcription factor regulating the expression of several downstream, abiotic-stress responsive gene(s). In this study, eight independent transgenic peanut (cv. GG20) lines were developed using AtDREB1A gene, encoding for a transcription factor, through Agrobacterium-mediated genetic transformation. The transgene insertion was confirmed in (T0) using PCR and Dot-blot analysis, while copy-number(s) was ascertained using Southern-blot analysis. The inheritance of AtDREB1A gene in individual transgenic plants (T1 and T2) was confirmed using PCR. In homozygous transgenic plants (T2), under soil-moisture deficit stress, elevated level of AtDREB1A transgene expression was observed by RT-PCR assay. The transgenic plants at 45-d or reproductive growth stage showed tolerance to severe soil-moisture deficit stress. Physio-biochemical parameters such as proline content, osmotic potential, relative water content, electrolytic leakage, and total-chlorophyll content were found positively correlated with growth-related traits without any morphological abnormality, when compared to wild-type. qPCR analysis revealed consistent increase in expression of AtDREB1A gene under progressive soil-moisture deficit stress in two homozygous transgenic plants. The transgene expression showed significant correlation with improved physio-biochemical traits. The improvement of drought-stress tolerance in combination with improved growth-related traits is very essential criterion for a premium peanut cultivar like GG20, so that marginal farmers of India can incur the economic benefits during seasonal drought and water scarcity. PMID:27446163

  5. Boiling and Frying Peanuts Decreases Soluble Peanut (Arachis Hypogaea Allergens Ara h 1 and Ara h 2 But Does Not Generate Hypoallergenic Peanuts.

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    Sarah S Comstock

    Full Text Available Peanut allergy continues to be a problem in most developed countries of the world. We sought a processing method that would alter allergenic peanut proteins, such that allergen recognition by IgE from allergic individuals would be significantly reduced or eliminated. Such a method would render accidental exposures to trace amounts of peanuts safer. A combination of boiling and frying decreased recovery of Ara h 1 and Ara h 2 at their expected MWs. In contrast, treatment with high pressures under varying temperatures had no effect on protein extraction profiles. Antibodies specific for Ara h 1, Ara h 2, and Ara h 6 bound proteins extracted from raw samples but not in boiled/fried samples. However, pre-incubation of serum with boiled/fried extract removed most raw peanut-reactive IgE from solution, including IgE directed to Ara h 1 and 2. Thus, this method of processing is unlikely to generate a peanut product tolerated by peanut allergic patients. Importantly, variability in individual patients' IgE repertoires may mean that some patients' IgE would bind fewer polypeptides in the sequentially processed seed.

  6. Boiling and Frying Peanuts Decreases Soluble Peanut (Arachis Hypogaea) Allergens Ara h 1 and Ara h 2 But Does Not Generate Hypoallergenic Peanuts

    OpenAIRE

    Comstock, Sarah S.; Maleki, Soheila J.; Teuber, Suzanne S.

    2016-01-01

    Peanut allergy continues to be a problem in most developed countries of the world. We sought a processing method that would alter allergenic peanut proteins, such that allergen recognition by IgE from allergic individuals would be significantly reduced or eliminated. Such a method would render accidental exposures to trace amounts of peanuts safer. A combination of boiling and frying decreased recovery of Ara h 1 and Ara h 2 at their expected MWs. In contrast, treatment with high pressures un...

  7. Boiling and Frying Peanuts Decreases Soluble Peanut (Arachis Hypogaea) Allergens Ara h 1 and Ara h 2 But Does Not Generate Hypoallergenic Peanuts.

    Science.gov (United States)

    Comstock, Sarah S; Maleki, Soheila J; Teuber, Suzanne S

    2016-01-01

    Peanut allergy continues to be a problem in most developed countries of the world. We sought a processing method that would alter allergenic peanut proteins, such that allergen recognition by IgE from allergic individuals would be significantly reduced or eliminated. Such a method would render accidental exposures to trace amounts of peanuts safer. A combination of boiling and frying decreased recovery of Ara h 1 and Ara h 2 at their expected MWs. In contrast, treatment with high pressures under varying temperatures had no effect on protein extraction profiles. Antibodies specific for Ara h 1, Ara h 2, and Ara h 6 bound proteins extracted from raw samples but not in boiled/fried samples. However, pre-incubation of serum with boiled/fried extract removed most raw peanut-reactive IgE from solution, including IgE directed to Ara h 1 and 2. Thus, this method of processing is unlikely to generate a peanut product tolerated by peanut allergic patients. Importantly, variability in individual patients' IgE repertoires may mean that some patients' IgE would bind fewer polypeptides in the sequentially processed seed. PMID:27310538

  8. The effect of temperature on radiation-induced macromutants of Arachis hypogaea L. and expression of heterosis in F1 hybrid populations

    International Nuclear Information System (INIS)

    Three separate experiments were conducted with cultivated peanuts to (a) study the effect of four controlled temperatures on the expression of seven radiation-induced macromutants and the mother line; (b) determine the responses of normal appearing F1 hybrids involving four macromutants to three controlled temperature regimes and (c) evaluate the same macromutant, parental and F1 hybrid populations (described in b) when grown for a full season under field conditions. Macromutant plant weights and flower productions were generally lower than that of the mother line in all three experiments. The macromutants also tended to flower later than the mother line. The leaflet expression of two of the macromutants was drastically influenced by changes in temperature. Lupinus reached maximum leaflet curvature when grown at 34/300C but appeared near normal when grown at 22/180C. The reverse was true for the macromutant Recurved. The macromutant Flop contained maximum percentages of nitrogen and phosphorus in its vegetative parts when grown at the highest temperature (34/300C). All other mutants and the mother line had highest proportions of these nutrients when grown at the lowest temperature regime (22/180C). Expressions of heterosis were maximized when hybrids of macromutant parentage were grown at sub-optimal temperatures. General combining ability estimates were higher than specific combining ability estimates with the macromutant Hedera (a dwarf type) making the greatest contribution to the general combining ability effects for peg (fruit-bearing structure) production and pegging efficiency. The same macromutant was found to make substantial contributions to general combining ability effects for fruit and seed weight when hybrids were grown under field conditions. (author)

  9. Study on factitious levels of 131 iodine contamination on its uptake and soil to crop transfer in groundnut (Arachis Hypogaea L.)

    International Nuclear Information System (INIS)

    An investigation was carried out to determine iodine-131 uptake and transfer factor (TF) in groundnut, pot-grown on three soils of varying texture, viz., a clay loam, a sandy clay loam and a sandy soil. The soils were factitiously contaminated with 131I at the rate 20, 40, 60, and 80 kBq kg-1 of soil. The effect of level of 131I was significant in the case of 131I uptake and TF. The 131I uptake increased significantly with the increasing levels of 131I and recorded a peak at the contamination level of 80 kBq kg-1 of soil. This was so in other plant parts as stem, leaf and shell and in whole plant as well. The uptake by kernel was significantly influenced by the soil texture. The values were lowest in the sandy soil and highest in sandy clay loam and sandy loam soils. The TF values in all plant parts decreased significantly with the 131I contamination levels. The TF values were significantly higher in roots than in the above-ground parts, amongst which the lowest TF was in the edible part kernel. (author)

  10. Application of natural adsorbent from silver impregnated Arachis hypogaea based thereon in the processes of hexavalent chromium for the purification of water

    International Nuclear Information System (INIS)

    Different industries generate huge amounts of chromium, both in solid and liquid form which leached out and find their way to the aquifers. Here we report the removal of Cr(VI) by the silver impregnated activated carbon developed from agricultural waste material at pH 3 from drinking water. Result revealed that adsorption of chromium(VI) on silver impregnated groundnut husk carbon was endothermic in nature. Thermodynamic parameters such as the entropy change, enthalpy change and Gibbs free energy change were found to be 1.68 kJ mol-1 K-1, 0.46 kJ mol-1 and -4.38 kJ mol-1, respectively. The measured adsorption kinetics is well described by a pseudo first-order kinetic model. The effect of flow rate, column depth and initial concentration of chromium(VI) on the removal of chromium by the impregnated adsorbent is presented. Scanning Electron Microscope (SEM), Electron Dispersive X-ray Analysis (EDX) and FTIR analysis show that the silver is impregnated on the surface. Shifts in the FTIR spectra suggest that dichromate binding occurs with silver and other functional groups and that silver treated groundnut husk carbon removes chromium. The 96 h static acute toxicity test was conducted to ensure the safe disposal of the used adsorbent.

  11. Changes in growth and yield characters and in genetic variation of peanut (Arachis hypogaea L.) plants due to gamma ray irradiation

    International Nuclear Information System (INIS)

    Air dried seeds of two peanut cultivars Giza 4 and Giza 5 were subjected to irradiation treatments of Co60 gamma ray doses i.e. 0, 100, 150, 200, 250 Gy to study their effect on growth characters, yield components, genetic variation, heritability and genetic advance for election; during 2000 and 2001 summer seasons. Results indicated that, the 100 Gy treatment produced the highest means of most growth characters in M1 and M2 generations, however the 250 Gy treatment produced the highest means for No. of pods/plant, pod yield/plant, seed yield/plant and shelling percentage in M1 generation, but the 200 Gy treatment produced the highest means of yield components in M2 generation for the two cultivars Giza 4 Giza 5. In general, mean percentages of oil and protein were decreased by increasing gamma ray doses in M1 and M2 generations for both Giza 4 and Giza 5. The highest estimates of phenotypic and genotypic coefficient of variation, heritability and genetic advance under selection were obtained with 250 Gy dose for most growth characters and yield components as well as oil and protein percentages of the two cultivars in both M1 and M2 generations

  12. Genetic mapping of QTLs controlling fatty acids provided insights into the genetic control of fatty acid synthesis pathway in peanut (Arachis hypogaea L..

    Directory of Open Access Journals (Sweden)

    Ming Li Wang

    Full Text Available Peanut, a high-oil crop with about 50% oil content, is either crushed for oil or used as edible products. Fatty acid composition determines the oil quality which has high relevance to consumer health, flavor, and shelf life of commercial products. In addition to the major fatty acids, oleic acid (C18:1 and linoleic acid (C18:2 accounting for about 80% of peanut oil, the six other fatty acids namely palmitic acid (C16:0, stearic acid (C18:0, arachidic acid (C20:0, gadoleic acid (C20:1, behenic acid (C22:0, and lignoceric acid (C24:0 are accounted for the rest 20%. To determine the genetic basis and to improve further understanding on effect of FAD2 genes on these fatty acids, two recombinant inbred line (RIL populations namely S-population (high oleic line 'SunOleic 97R' × low oleic line 'NC94022' and T-population (normal oleic line 'Tifrunner' × low oleic line 'GT-C20' were developed. Genetic maps with 206 and 378 marker loci for the S- and the T-population, respectively were used for quantitative trait locus (QTL analysis. As a result, a total of 164 main-effect (M-QTLs and 27 epistatic (E-QTLs QTLs associated with the minor fatty acids were identified with 0.16% to 40.56% phenotypic variation explained (PVE. Thirty four major QTLs (>10% of PVE mapped on five linkage groups and 28 clusters containing more than three QTLs were also identified. These results suggest that the major QTLs with large additive effects would play an important role in controlling composition of these minor fatty acids in addition to the oleic and linoleic acids in peanut oil. The interrelationship among these fatty acids should be considered while breeding for improved peanut genotypes with good oil quality and desired fatty acid composition.

  13. Studies on radiation induced effects in homozygous and heterozygous genotypes of groundnut (Arachis hypogaea L.) 1. combining ability analysis on reproductive characters

    International Nuclear Information System (INIS)

    The magnitude of the variance due to general combining ability (gca) was more for number of flowers, number of pegs, number of mature pods and reproductive efficiency and that of specific combining ability (sca) for ratio of pegs to flowers in the non-irradiated treatments. In the case of irradiated treatments the magnitude of gca was more in all the characters except number of pegs. Differences in the gca and sca effects of parents and F 1 hybrids were noticed due to irradiation

  14. Fertilizer nitrogen recovery from different soil depths in an alley cropping system consisting of peanut (Arachis hypogaea) and Choerospondias axillaris in subtropical China

    International Nuclear Information System (INIS)

    Tree and crop components of an alley cropping system may compete for N in the surface soil while the tree may use leached N in the deep soil not accessed by the crop. The objective of the present study was to evaluate the recovery of nitrogen by placing 15N fertilizer at different soil depths to assess the competitiveness for N between tree and crop in an alley cropping system, consisting of peanut and Choerospondias axillaris. The tree competed with peanut for N in the surface soil as indicated by the lower 15N recovery by peanut and the higher 15N recovery in the tree in the alley cropping system, and by a higher N content in the tree mono treatment. The tree can use leached N in the deep soil as indicated by the high 15N recovery when 15N was placed at 60 cm depth. Usage of N in the deep soil was consistent with the deeper tree root distribution in the alley cropping system as compared to that in the tree alone system. The magnitudes of these changes were larger for the system with larger trees and increased with time. The alley cropping system increased system N use efficiency compared with the peanut mono cropping system. The N use efficiency increased as the tree grew as indicated by the increased 15N recovery. These results suggest that introduction of trees can reduce N loss in cropping systems, but management should aim to prevent N competition with the crop in the surface soil. (author)

  15. Exogenous calcium alleviates photoinhibition of PSII by improving the xanthophyll cycle in peanut (Arachis hypogaea leaves during heat stress under high irradiance.

    Directory of Open Access Journals (Sweden)

    Sha Yang

    Full Text Available Peanut is one of the calciphilous plants. Calcium (Ca serves as a ubiquitous central hub in a large number of signaling pathways. The effect of exogenous calcium nitrate [Ca(NO32] (6 mM on the dissipation of excess excitation energy in the photosystem II (PSII antenna, especially on the level of D1 protein and the xanthophyll cycle in peanut plants under heat (40°C and high irradiance (HI (1 200 µmol m(-2 s(-1 stress were investigated. Compared with the control plants [cultivated in 0 mM Ca(NO32 medium], the maximal photochemical efficiency of PSII (Fv/Fm in Ca(2+-treated plants showed a slighter decrease after 5 h of stress, accompanied by higher non-photochemical quenching (NPQ, higher expression of antioxidative genes and less reactive oxygen species (ROS accumulation. Meanwhile, higher content of D1 protein and higher ratio of (A+Z/(V+A+Z were also detected in Ca(2+-treated plants under such stress. These results showed that Ca(2+ could help protect the peanut photosynthetic system from severe photoinhibition under heat and HI stress by accelerating the repair of D1 protein and improving the de-epoxidation ratio of the xanthophyll cycle. Furthermore, EGTA (a chelant of Ca ion, LaCl3 (a blocker of Ca(2+ channel in cytoplasmic membrane, and CPZ [a calmodulin (CaM antagonist] were used to analyze the effects of Ca(2+/CaM on the variation of (A+Z/(V+A+Z (% and the expression of violaxanthin de-epoxidase (VDE. The results indicated that CaM, an important component of the Ca(2+ signal transduction pathway, mediated the expression of the VDE gene in the presence of Ca to improve the xanthophyll cycle.

  16. The potential of Arachis pintoi biomass to improve quality of soil continuously used for cassava cropping

    Directory of Open Access Journals (Sweden)

    N. Muddarisna

    2014-01-01

    Full Text Available A field experiment that was aimed to elucidate the effects of application of Arachis pintoi biomass and animal dung on quality of soil continuously used for cassava cropping was conducted at Jatikerto Village, Kromengan District of Malang Regency. Eight treatments tested were 100% NPK inorganic fertilizer, 100 kg N Arachis pintoi/ha, (3 100 kg N chicken dung/ ha, 100 kg N cow dung /ha, 100 kg N goat dung /ha, 100 kg N Arachis pintoi + chicken dung /ha, 100 kg N Arachis pintoi + cow dung /ha, and 100 kg N Arachis pintoi + goat dung /ha. Monitoring quality of top soil (0-20 cm was carried out at planting time and 3 months after planting. Soil samples were collected and analyzed for chemical and physical properties. Yield of cassava was measured at 6 months after planting. Results of this study showed that application of organic fertilizer in forms of green manure (Arachis pintoi biomass, and animal dung significantly improved physical and chemical properties of soil. Application of 50% NPK combined with organic manures did not significantly gave different tuber yield with that of 100% NPK.

  17. Nitrogen fertilization on the establishment of Arachis pintoi cv. Belmonte

    Directory of Open Access Journals (Sweden)

    Rita Manuele Porto Sales

    2012-11-01

    Full Text Available The objective was to evaluate the effect of nitrogen fertilization on the establishment of forage peanut (Arachis pintoi cv. Belmonte propagated vegetatively. The experiment was conducted in a greenhouse in a completely randomized design with treatments arranged in a 2 × 4 factorial design - two ages (70 and 85 days after planting and four nitrogen doses (0, 40, 80 and 120 kg/ha - with four replications. Morphogenetic and structural characteristics and production were evaluated. The nitrogen accelerated the establishment of the forage peanut with an increase in dry weight of green leaves and stolons. The greatest length of stolons (48.0 cm was obtained with a dose equivalent to 86 kg N/ha and higher density of stolons (20 stolons/vase between 78 and 82 kg N/ha. Nitrogen fertilization also reduced the phyllochron from 6.7 to 4.6 days/leaf. These data were more intense at 85 days, suggesting greater photosynthetic contribution during this period related to the large number of leaves after 70 days. Therefore, nitrogen can be an important tool to accelerate the establishment of pure stands of forage peanut.

  18. Seletividade do imazapic para dois cultivares de amendoim (Arachis hypogaea cultivados na ausência e na presença de palha de cana-de-açúcar Imazapic selectivity on initial development of peanut (Arachis hypogaea with and without sugarcane straw

    Directory of Open Access Journals (Sweden)

    C.A.M. Azania

    2004-03-01

    Full Text Available Neste trabalho, objetivou-se estudar a seletividade de imazapic a dois cultivares de amendoim (Tatu Vermelho e IAC-5, em condições de ausência e de presença de palha de cana-de-açúcar. O experimento foi instalado em vasos, em delineamento inteiramente casualizado, com os tratamentos dispostos em esquema fatorial 3x2, com quatro repetições. Constituíram os fatores principais três doses de imazapic (0, 98 e 140 g ha-1, aplicadas em pré-emergência sobre duas condições de palha de cana-de-açúcar (0 e 15 t ha-1. Constatou-se que a camada de palha possui potencial em reter o herbicida, especialmente sob períodos de pouca precipitação ou irrigação; as plantas de amendoim sob esta condição apresentaram menos sintomas de intoxicação. Entretanto, na ausência da palha, as plantas, independentemente do cultivar, apresentaram maiores injúrias visuais e redução na altura, massa seca da parte aérea e raiz.This research aimed to evaluate imazapic selectivity for two peanut cultivars (Tatu Vermelho and IAC with and without sugarcane straw. The experimental design was completely randomized arranged in a3x2 factorial scheme with four replicates. The experiment was set in plastic pots and the treatments consisted of a combination of three rates of imazapic applied in pre-emergence (0, 98, and 140 g ha-1, and two levels of sugarcane straw (0 and 15 ton ha-1. The straw layer retained the herbicide, mainly under little precipitation or irrigation periods; under these conditions, peanut plants showed fewer intoxication symptoms. However, without the straw,the plants showed larger visual injuries and reduction in height, aerial parts and root dry mass for both peanut cultivars and herbicide rates.

  19. Use of EST-SSR loci flanking regions for phylogenetic analysis of genus Arachis

    Science.gov (United States)

    All wild peanut collections in the genus Arachis were assigned to nine taxonomy sections on the bases of cross-compatibility and morphologic character clustering. These nine sections consist of 80 species from the most ancient to the most advanced, providing a diverse genetic resource for phylogenet...

  20. Introgression of the SbASR-1 gene cloned from a halophyte Salicornia brachiate enhances salinity and drought endurance in transgenic groundnut (arachis hypogaeaand acts as a transcription factor [corrected].

    Directory of Open Access Journals (Sweden)

    Vivekanand Tiwari

    Full Text Available The SbASR-1 gene, cloned from a halophyte Salicornia brachiata, encodes a plant-specific hydrophilic and stress responsive protein. The genome of S. brachiata has two paralogs of the SbASR-1 gene (2549 bp, which is comprised of a single intron of 1611 bp, the largest intron of the  abscisic acid stress ripening [ASR] gene family yet reported. In silico analysis of the 843-bp putative promoter revealed the presence of ABA, biotic stress, dehydration, phytohormone, salinity, and sugar responsive cis-regulatory motifs. The SbASR-1 protein belongs to Group 7 LEA protein family with different amino acid composition compared to their glycophytic homologs. Bipartite Nuclear Localization Signal (NLS was found on the C-terminal end of protein and localization study confirmed that SbASR-1 is a nuclear protein. Furthermore, transgenic groundnut (Arachis hypogaea plants over-expressing the SbASR-1 gene constitutively showed enhanced salinity and drought stress tolerance in the T1 generation. Leaves of transgenic lines exhibited higher chlorophyll and relative water contents and lower electrolyte leakage, malondialdehyde content, proline, sugars, and starch accumulation under stress treatments than wild-type (Wt plants. Also, lower accumulation of H2O2 and O2.- radicals was detected in transgenic lines compared to Wt plants under stress conditions. Transcript expression of APX (ascorbate peroxidase and CAT (catalase genes were higher in Wt plants, whereas the SOD (superoxide dismutase transcripts were higher in transgenic lines under stress. Electrophoretic mobility shift assay (EMSA confirmed that the SbASR-1 protein binds at the consensus sequence (C/G/A(G/TCC(C/G(C/G/A(A/T. Based on results of the present study, it may be concluded that SbASR-1 enhances the salinity and drought stress tolerance in transgenic groundnut by functioning as a LEA (late embryogenesis abundant protein and a transcription factor.

  1. Comparisons of De Novo Transcriptome Assemblers in Diploid and Polyploid Species Using Peanut (Arachis spp.) RNA-Seq Data

    OpenAIRE

    Chopra, Ratan; Burow, Gloria; Farmer, Andrew; Mudge, Joann; Charles E. Simpson; Burow, Mark D.

    2014-01-01

    The narrow genetic base and limited genetic information on Arachis species have hindered the process of marker-assisted selection of peanut cultivars. However, recent developments in sequencing technologies have expanded opportunities to exploit genetic resources, and at lower cost. To use the genetic information for Arachis species available at the transcriptome level, it is important to have a good quality reference transcriptome. The available Tifrunner 454 FLEX transcriptome sequences hav...

  2. Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers.

    Science.gov (United States)

    Palmieri, Darío A; Bechara, Marcelo D; Curi, Rogério A; Monteiro, Jomar P; Valente, Sérgio E S; Gimenes, Marcos A; Lopes, Catalina R

    2010-01-01

    Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae germplasm, as well as that of species belonging to other Arachis sections. PMID:21637613

  3. Genetic diversity analysis in the section Caulorrhizae (genus Arachis using microsatellite markers

    Directory of Open Access Journals (Sweden)

    Darío A. Palmieri

    2010-01-01

    Full Text Available Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. & W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae germplasm, as well as that of species belonging to other Arachis sections.

  4. Phylogenetic relationships in genus Arachis based on ITS and 5.8S rDNA sequences

    Directory of Open Access Journals (Sweden)

    Valls José FM

    2010-11-01

    Full Text Available Abstract Background The genus Arachis comprises 80 species and it is subdivided into nine taxonomic sections (Arachis, Caulorrhizae, Erectoides, Extranervosae, Heteranthae, Procumbentes, Rhizomatosae, Trierectoides, and Triseminatae. This genus is naturally confined to South America and most of its species are native to Brazil. In order to provide a better understanding of the evolution of the genus, we reconstructed the phylogeny of 45 species using the variation observed on nucleotide sequences in internal transcribed spacer regions (ITS1 and ITS2 and 5.8 S of nuclear ribosomal DNA. Results Intraspecific variation was detected, but in general it was not enough to place accessions of the same species in different clades. Our data support the view that Arachis is a monophyletic group and suggested Heteranthae as the most primitive section of genus Arachis. The results confirmed the circumscriptions of some sections (Caulorrhizae, Extranervosae, but raised questions about others. Sections Erectoides, Trierectoides and Procumbentes were not well defined, while sections Arachis and Rhizomatosae seem to include species that could be moved to different sections. The division of section Arachis into A and B genome species was also observed in the phylogenetic tree and these two groups of species may not have a monophyletic origin. The 2n = 2x = 18 species of section Arachis (A. praecox, A. palustris and A. decora were all placed in the same clade, indicating they are closely related to each other, and their genomes are more related to B genome than to the A genome. Data also allowed insights on the origin of tetraploid A. glabrata, suggesting rhizome appeared twice within the genus and raising questions about the placement of that species in section Rhizomatosae. Conclusion The main clades established in this study in general agreed with many other studies that have used other types of evidences and sets of species, being some of them included in our

  5. Evaluation of Different Groundnut Arachis hypogea L. Cultivars Against Termites, Odontotermes obesus (Rambur) in Rawalpindi, Pakistan

    OpenAIRE

    Muhammad Usman Malik; Humayun Javed; Mahmood Ayyaz

    2015-01-01

    Six groundnut (Arachis hypogea L.) cultivars i.e. Golden, BARI-2000, Australian, BARD-479, ICG-7326 and 2KCGC20 were evaluated against the attack of Odontotermes obesus in terms of percentage infestation of workers and soldiers, their damage and impact on the yield. The maximum mean populations of O. obesus workers were recorded on 2KCGC20 (30.82±2.75 workers/plot) and Australian (30.65±2.92 workers/plot), followed by BARI 2000 (19.55±2.51 workers/plot), Golden (17.30±2.27 workers/plot) and B...

  6. Effect of water stress on the growth of Arachis pintoi plants under different nitrogen levels

    OpenAIRE

    Rita Manuele Porto Sales; Daniela Deitos Fries; Paulo Bonomo; Aureliano José Vieira Pires; Cleide Nascimento Campos; Paulo Henrique Rodrigues Brito; Iracema Souza Pereira; Joelma da Silva Santos

    2013-01-01

    The objective was to evaluate the growth of Arachis pintoi fertilized with nitrogen and water deficiency conditions. The study was developed in a 4 × 4 factorial arrangement with four nitrogen levels (0, 40, 80 and 120 kg N/ha) and four irrigation levels (25, 50, 75 and 100% of field capacity), in a completely randomized experimental design, with four replications. High doses of N benefited the production of root shoots while the root increased its production at a dose of 40 kg N/ha. The numb...

  7. ESTs from a wild Arachis species for gene discovery and marker development

    OpenAIRE

    da Silva Felipe R; Moretzsohn Márcio C; Bertioli David J; Leal-Bertioli Soraya CM; Proite Karina; Martins Natalia F; Guimarães Patrícia M

    2007-01-01

    Abstract Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries of RNAs extracted from leaves and roots of A. stenosperma. Randomly selected cDNA clones were sequenced to generate 8,785 ESTs, of which 6,264 (71.3%) had high quality, with 3,500 ...

  8. Comparisons of de novo transcriptome assemblers in diploid and polyploid species using peanut (Arachis spp.) RNA-seq data

    Science.gov (United States)

    The narrow genetic base and limited genetic information on Arachis species have hindered the process of marker-assisted selection of peanut cultivars. However, recent developments in sequencing technologies have expanded opportunities to exploit genetic resources, and at lower cost. To use the genet...

  9. Improving a native pasture with the legume Arachis pintoi in the humid tropics of México

    NARCIS (Netherlands)

    Castillo Gallegos, E.

    2003-01-01

    The objective of this study was to determine the effect of introducing the legume Arachis pintoi CIAT 17434 into a native pasture where native grasses dominated the botanical composition, on establishment, persistence, standing dry matter, botanical composition, soil variables, animal performance, h

  10. Study on Competition Characteristics of a Land Cover Crop Arachis pintoito Growth of Cocoa

    Directory of Open Access Journals (Sweden)

    John Bako Baon

    2006-12-01

    Full Text Available The existence of ground cover crop, such as Arachis pintoiin cocoa farm may enhance physical, chemistry and biological fertility of soil. Nevertheless, the presence of those cover crops potentially increase the competition with main crop in various means. The primary objective of this study was to investigate kinds of competition raised when cocoa plants cultivated with ground cover crop of A. pintoi. This study consisted of three experiments. The first experiment aimed to study possible allelopatic competition using root exudates solution of A. Pintoi as well as Leucaena leucocephalaand Adenanthera microsperma as check for being used for watering cocoa plants. The second experiment aimed to study the competition between A. pintoiand cocoa plants in field condition with 1 cover crop fully overlay ground surrounding cocoa basal trunk, 2 no cover crop overlay ground surrounding cocoa basal trunk in a distance of 40 cm in diameter, and 3 no cover crop as check. Third experiment aimed to study the competition between A. pintoiand cocoa plants in limited growth medium (pot, using 10 cm stolon shoots each 4, 2 and 0 shoots per pot. Results showed that A. Pintoidid not produce alleopatic exudates to restrict cocoa growth. Population of A. Pintoi had negative effect on cocoa plant growth in the field by reducing fresh trunk weight and dry root weight, even greater negative effect found in cocoa plant grown in limited growth medium by also reducing dry leaf and trunk weight and leaf area. Key words: Theobroma cacao, Arachis pintoi, allelopathy, competition, denantheramicrosperma, Leucaena leucocephala

  11. Evaluación de la resistencia a sequía de variedades de cacahuate (Arachis hypogaea L.) de hábito de crecimiento rastrero y erecto

    OpenAIRE

    A Muñoz-Orozco; V. A. González-Hernández; S. Sánchez-Domínguez

    2006-01-01

    Para evaluar la resistencia a sequía del cacahuate se realizó un experimento en el que se probaron dos hábitos de crecimiento: rastrero y erecto con ocho variedades anidadas en cada hábito, bajo dos niveles de humedad. Se midieron 22 características vegetativas y reproductivas. En número de ginóforos, vainas inmaduras, peso de semilla, intensidad del color verde de la hoja y altura de planta existieron diferencias estadísticas entre los dos niveles de humedad. Altura de planta fue superior ba...

  12. 栽培种花生荚果大小相关性状QTL定位%Mapping of QTLs for Pod Size Related Traits in Cultivated Peanut (Arachis hypogaeaL.)

    Institute of Scientific and Technical Information of China (English)

    李振动; 李新平; 黄莉; 任小平; 陈玉宁; 周小静; 廖伯寿; 姜慧芳

    2015-01-01

    以远杂9102为母本,徐州68-4为父本杂交衍生的 F5和F6共188个家系,构建了一张包含365个标记,总长度713.07 cM,标记间平均距离1.96 cM的栽培种花生遗传图谱。图谱包含22个连锁群,各连锁群平均长度12.37~81.39 cM,连锁群上标记数量3~46个。结合2013和2014年采集的荚果表型数据,采用WinQTLcart 2.5软件的复合区间作图法(composite interval mapping, CIM)进行QTL定位和效应估计。2个环境下共检测到41个QTL,其中与荚果长、宽、厚和百果重相关的QTL分别为13、7、13和8个,表型变异解释率为3.14%~18.27%。有6个QTL在2种环境下被重复检测到,其中百果重相关的2个(qHPWLG13.1、qHPWLG14.1),分布在LG13和LG14连锁群,遗传贡献率为6.95%~14.60%;与荚果长相关的3个(qLPLG2.2、qLPLG13.1、qLPLG14.1),分布在 LG2、LG13和 LG14连锁群,遗传贡献率为3.14%~18.27%;与荚果厚相关的1个(qTPLG3.4),分布在 LG3连锁群,遗传贡献率为8.24%~9.24%。本研究涉及性状存在9个QTL热点区,每个热点区涉及2~3个性状,表型贡献率为3.57%~18.27%。%One hundred and eighty-eight recombinant inbred lines (RIL), derived from a cross between two Spanish type peanut cultivars (Yuanza 9102 × Xuzhou 68-4), were used as mapping population. Finally, a genetic linkage map consisting of 443 SSR loci in 22 linkage groups and covering 713.07 cM with an average distance of 1.96 cM was constructed. The length of linkage group was from 12.37 cM to 81.39 cM and the number of markers was 3-46. QTL mapping of the traits related to pod was con-ducted by using CIM model in WinQTLcart 2.5. A total of 41 QTLs were detected in the two environments, including thirteen for pod length, seven for pod width, thirteen for pod thickness and right for hundred pod weight, every single QTL explained 3.14%–18.27% of the phenotypic variation. A total of six QTLs were detected in both environments, including three for pod length with explained phenotypic variance of 3.14%–18.27% on the linkage group 2, linkage group 13 and linkage group 14. One for pod thickness with explained phenotypic variance of 8.24%–9.24% on the linkage group 3, and two for hundred pod weight with explained phenotypic variance of 6.95%–14.60% on the linkage group 13 and linkage group 14. The result showed that there were nine hotsports for QTL research, and each of them was associated with 2–3 traits, explaining 3.57%–18.27% of the pheno-typic variation.

  13. Simultaneous analysis of herbicides pendimethalin, oxyfluorfen, imazethapyr and quizalofop-p-ethyl by LC-MS/MS and safety evaluation of their harvest time residues in peanut (Arachis hypogaea L.).

    Science.gov (United States)

    Saha, Ajoy; Shabeer T P, Ahammed; Banerjee, Kaushik; Hingmire, Sandip; Bhaduri, Debarati; Jain, N K; Utture, Sagar

    2015-07-01

    This paper reports a simple and rapid method for simultaneous determination of the residues of selected herbicides viz. pendimethalin, oxyfluorfen, imazethapyr and quizalofop-p-ethyl in peanut by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified approach of the QuEChERS methodology was used to extract the herbicides from the peanut kernel without any clean-up. The method showed excellent linearity (r(2) > 0.99) with no significant matrix effect. Accuracy of the method in terms of average recoveries of all the four herbicides ranged between 69.4 -94.4 % at spiking levels of 0.05, 0.10 and 0.25 mg kg(-1) with intra-day and inter-day precision RSD (%) between 2.6-16.6 and 8.0-11.3, respectively. Limit of quantification (LOQs) was 5.0 μg kg(-1) for pendimethalin, imazethapyr and quizalofop-p-ethyl and 10.0 μg kg(-1) for oxyfluorfen. The expanded uncertainties were <11 % for determination of these herbicides in peanut. The proposed method was successfully applied for analysis of these herbicide residues in peanut samples harvested from the experimental field and the residues were below the detection level. PMID:26139867

  14. Effects of Controlled-Release Fertilizers on Leaf Physiology of Different Peanut Varieties%控释肥料对不同品种花生(Arachis hypogaeaL.)叶片生理的影响

    Institute of Scientific and Technical Information of China (English)

    郭峰; 初长江; 王才斌; 吴正锋; 李新国; 郑亚萍; 万书波

    2012-01-01

    大田栽培条件下,以“花育22”和“花育20”品种为材料,以不施肥为对照,设控释肥675kg bm^-2控释肥506kg hm^-2和普通肥料3个处理,研究了控释肥料对不同品种花生叶片衰老的影响。结果表明:相比普通肥料,控释肥可以提高花生生育后期叶面积系数、叶绿素含量以及有关保护酶的活性,降低叶片膜脂过氧化程度,有效延缓花生的衰老速率,提高生育后期光合产物积累,为花生产量的提高奠定基础。因此,控释肥是花生较为理想的肥料。%Field experiments were carried out to study the effects of controlled-release fertilizers on leaf physiology of different peanut varieties, 'Huayu 22' and 'Huayu 20'. There were four treatments: controlled-release fertilizer 675 kg hm^-2 (T1), controlled-release fertilizer 506 kg hm^-2 (T2), ordinary fertilizer (T3) and without fertilization (T4). Controlled-release fertilizers could improve LAI, Chl as well as activities of SOD, POD and CAT decrease the Lipid peroxidation of leaf, delay the senescence and enhance the accumulation of photosynthetic product during the later growing stage of peanut. Relative to T3 treatment, controlled-release fertilizer treatments were better on delaying plant senescence. These results implied that controlled-release fertilizers could contribute the delaying of the leave senescence and the accumulation of photosynthetic product during the later growing stage of peanut plant, and were suitable for peanut growth and development.

  15. Ocorrência de larvas de Diabrotica speciosa (Germar, 1824 danificando vagens e sistema radicular de amendoinzeiro Occurrence of larvae op Diabrotica speciosa (Germar, 1824 damaging pods and roots op peanut (Arachis hypogaea L.

    Directory of Open Access Journals (Sweden)

    André Luiz Lourenção

    1982-01-01

    Full Text Available In April 1982, at Urânia, State of São Paulo, Brazil, a high infestation of larvae of Diabrotica speciosa (Germar, 1824 (Coleoptera: Chrysomelidae damaging roots, gynophores and pods of an irrigated peanut crop was observed. The roots, radicles and gynophores showed lesions due to the attack of the larvae; sometimes these plant parts were completely destroyed. The pods presented holes through which the larvae penetrated in order to eat the seeds. Because of the high infestation and the heavy damage the harvest of the crop was considered not economical.

  16. Contribución relativa del nitrógeno del suelo y del fijado biológicamente a la economía de la nutrición nitrogenada de maní (Arachis hypogaea L. en diferentes condiciones de fertilidad Relative contribution of biological fixed nitrogen and soil nitrogen to the nutrition economy of peanut (Arachis hypogaea L. under different conditions of soil fertility

    Directory of Open Access Journals (Sweden)

    S. Castro

    2006-12-01

    Full Text Available La producción de maní en Argentina se concentra en la región central de la provincia de Córdoba, la cual experimentó últimamente una pérdida importante de la productividad de los suelos y una declinación aleatoria del rendimiento de los cultivos. La contribución relativa de la fijación biológica (FBN de nitrógeno al maní en suelos de diferente fertilidad no ha sido suficientemente estudiada. Entonces, se evaluó el efecto de cepas de rizobios (TTOO2R, SEMIA 6144R y TAL 1000R sobre el rendimiento y el balance de nitrógeno de maní cultivado en suelos con alto y bajo contenido del nutriente. No hubo diferencias significativas en los parámetros simbióticos y de rendimiento del cultivo entre las cepas introducidas y las nativas, pero se observó una contribución relativa mayor de la FBN en el suelo con bajo contenido de nitrógeno (~58% de contribución que en el suelo con alto contenido (~27% de contribución. Esta comprobación del aporte relativo de la FBN asociada a la fertilidad del suelo, no registra antecedentes en la región central de Córdoba y debería recibir mayor consideración en el manejo del cultivo particularmente por su localización actual al sur de la provincia, donde los suelos presentan menores niveles de fertilidad. El rendimiento de maní confitería mostró mayores valores, si bien no significativos, con la inoculación en los 3 años del estudio.The peanut production in Argentina is concentrated in the central region of Córdoba province. At present, losses of soil fertility and a random decline peanut yield have been reported for this area. The relative contribution of biological nitrogen fixation (FBN in peanut plants cropped in soils with different fertility, has not been extensively studied. An experiment was carried out to determine the effects of rhizobia strains (TTOO2R, SEMIA 6144R and TAL 1000R on peanut crop yield and plant nitrogen balance under different conditions of soil nitrogen. The results did not show significant differences in the symbiotic parameters and peanut crop yield between native and non-native strains. However, a relative higher contribution of biological nitrogen fixation was observed in low nitrogen (~58% contribution than in high nitrogen soil content (~27% contribution. The relative contribution of FBN associated with soil fertility has not been investigated in the central region of Córdoba and it becomes particularly important in crop management for the current Southern cropping region where soils have lower fertility levels. The peanut crop showed a confectionary higher yield, although no significant, during the three years of experiments.

  17. High-oleic Virginia peanuts in the Southwestern US: A summary of data supporting the release of 'VENUS'

    Science.gov (United States)

    'VENUS' is a large-seeded high-oleic Virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) that has enhanced Sclerotinia blight and pod rot tolerance when compared to the cultivar Jupiter. 'VENUS' is the first high-oleic Virginia peanut developed for and proposed for release in t...

  18. Under the volcano: phylogeography and evolution of the cave-dwelling Palmorchestia hypogaea (Amphipoda, Crustacea at La Palma (Canary Islands

    Directory of Open Access Journals (Sweden)

    Oromí Pedro

    2008-01-01

    Full Text Available Abstract Background The amphipod crustacean Palmorchestia hypogaea occurs only in La Palma (Canary Islands and is one of the few terrestrial amphipods in the world that have adapted to a strictly troglobitic life in volcanic cave habitats. A surface-dwelling closely related species (Palmorchestia epigaea lives in the humid laurel forest on the same island. Previous studies have suggested that an ancestral littoral Orchestia species colonized the humid forests of La Palma and that subsequent drought episodes in the Canaries reduced the distribution of P. epigaea favouring the colonization of lava tubes through an adaptive shift. This was followed by dispersal via the hypogean crevicular system. Results P. hypogaea and P. epigaea did not form reciprocally monophyletic mitochondrial DNA clades. They showed geographically highly structured and genetically divergent populations with current gene flow limited to geographically close surface locations. Coalescence times using Bayesian estimations assuming a non-correlated relaxed clock with a normal prior distribution of the age of La Palma, together with the lack of association of habitat type with ancestral and recent haplotypes, suggest that their adaptation to cave life is relatively ancient. Conclusion The data gathered here provide evidence for multiple invasions of the volcanic cave systems that have acted as refuges. A re-evaluation of the taxonomic status of the extant species of Palmorchestia is needed, as the division of the two species by habitat and ecology is unnatural. The information obtained here, and that from previous studies on hypogean fauna, shows the importance of factors such as the uncoupling of morphological and genetic evolution, the role of climatic change and regressive evolution as key processes in leading to subterranean biodiversity.

  19. Produção e qualidade de massa de forragem nos estratos da cultivar coastcross-1 consorciada com Arachis pintoi com e sem adubação nitrogenada = Forage mass production and quality in coastcross-1 pasture layers, mixed with Arachis pintoi with or without nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    2008-04-01

    Full Text Available Neste trabalho, objetivou-se avaliar a massa de forragem nas frações lâminas foliares (LF, bainha + colmo verde (BCV, material morto (MM e seus teores de proteína bruta (PB e fibra em detergente neutro (FDN nos estratos de 0 a 7 cm, 7 a 14 cm e acima de 14 cm de altura da cultivar Coastcross-1 e planta inteira de Arachis pintoi (AP em pastejo, de março de 2003 a março de 2004. Estudaram-se os efeitos dos tratamentos CA0 = Coastcross-1 + Arachis sem N; CA100 = Coastcross-1 + Arachis com 100 kg de N; CA200 = Coastcross-1 + Arachis com 200 kg de N e C200 = Coastcross-1 com 200 kg deN, em um delineamento em blocos ao acaso, com duas repetições. O método de pastejo foi contínuo e a taxa de lotação, variável. As proporções de LF da gramínea Coastcross-1 aumentaram e de BCV, MM e AP diminuíram com o aumento da altura. Não foram observadas diferenças entre os tratamentos. A planta inteira da leguminosa Arachis teve pouca influência na composição da pastagem pela sua baixa disponibilidade. Os maiores (p This trial was carried out to evaluate forage mass in fraction leaf blade (LB, sheath + green stem (SGS, dead material (DE, and crude protein (CP percentage and neutral detergent fiber (NDF in thelayers of 0 to 7 cm, 7 to 14 cm and over 14 cm high. Coastcross-1 grass and the whole plant of Arachis pintoi (WPA were evaluated under grazing, from March 2003 to March 2004. The treatments evaluated were CA0 = Coastcross-1 + Arachis without N; CA100= Coastcross-1 + Arachis with 100 kg of N; CA200 = Coastcross-1 + Arachis with 200 kg of N; and C200 = Coastcross-1 with 200 kg of N, in a random block design, with two repetitions. The proportion of LB and SGS increased, while DE and WPA decreased with the increase of clipping height. No difference was observed among treatments. Arachis had little influence on pasture composition because of its low availability. The highest values (p < 0.05 for CP and the lowest values for NDF were observed in

  20. Fibre degradability of oil palm frond pellet, supplemented with Arachis pintoi in cattle

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    Bodee Khamseekhiew

    2002-04-01

    Full Text Available An experiment was conducted to evaluate the effect of different levels of Arachis pintoi (AP supplementation on rumen environment [(rumen pH, ruminal ammonia nitrogen (NH3N and volatile fatty acids (VFAs concentration] and degradability of oil palm frond (OPF. Three Kedah-Kelantan (KK cattle of about 2 1/2 years of age with an average body weight (BW173±17.2 kg, each fitted with a ruminal cannula, were used. The cattle were kept in individual pens and fed the treatment diets at 1.5% of BW. The diets comprised the following four OPF:AP ratios; 80:20 (L20, 70:30 (L30, 60:40 (L40, 50:50 (L50 in a 4 × 4 incomplete Latin Square Design. The DM an NDF degradation rates of OPF were significantly affected by AP supplementation. Ruminal pH was not significantly different (p>0.05 among the four different diets. The concentration of NH3N was significantly (p<0.05 higher in cattle fed L50 than those in L40, L30 and L20. Similarly, increasing levels of AP supplementation significantly increased the total VFAs concentration from 59.9 mmol/L for L20 to 69.2 mmol/L for L50. It is suggested that AP can be used as a protein supplement to improve fibre degradability of OPF in cattle.

  1. Effect of water stress on the growth of Arachis pintoi plants under different nitrogen levels

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    Rita Manuele Porto Sales

    2013-03-01

    Full Text Available The objective was to evaluate the growth of Arachis pintoi fertilized with nitrogen and water deficiency conditions. The study was developed in a 4 × 4 factorial arrangement with four nitrogen levels (0, 40, 80 and 120 kg N/ha and four irrigation levels (25, 50, 75 and 100% of field capacity, in a completely randomized experimental design, with four replications. High doses of N benefited the production of root shoots while the root increased its production at a dose of 40 kg N/ha. The number of green and senescent leaves were influenced by N fertilization and the water regime, where higher doses of N provided greater total number of green leaves (364 leaves/pot and the limitation of water resulted in a greater number of senescent leaves (78 leaves/pot. Observing the maximum point, the water regime of 90% of field capacity and 120 kg N/ha favored leaf appearance rate of 1.2 leaves/day and less phyllochron (4.6 days/leaf at 85% of field capacity and 115 kg N/ha. The dose of 120 kg N/ha resulted in ultimate greater width (1.4 cm and length (2.4 cm of the leaf at 100% of field capacity. The final length of stolons showed quadratic effect for N, having greatest length (58.1 cm at 75 kg N/ha and 100% of field capacity.

  2. ESTs from a wild Arachis species for gene discovery and marker development

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    da Silva Felipe R

    2007-02-01

    Full Text Available Abstract Background Due to its origin, peanut has a very narrow genetic background. Wild relatives can be a source of genetic variability for cultivated peanut. In this study, the transcriptome of the wild species Arachis stenosperma accession V10309 was analyzed. Results ESTs were produced from four cDNA libraries of RNAs extracted from leaves and roots of A. stenosperma. Randomly selected cDNA clones were sequenced to generate 8,785 ESTs, of which 6,264 (71.3% had high quality, with 3,500 clusters: 963 contigs and 2537 singlets. Only 55.9% matched homologous sequences of known genes. ESTs were classified into 23 different categories according to putative protein functions. Numerous sequences related to disease resistance, drought tolerance and human health were identified. Two hundred and six microsatellites were found and markers have been developed for 188 of these. The microsatellite profile was analyzed and compared to other transcribed and genomic sequence data. Conclusion This is, to date, the first report on the analysis of transcriptome of a wild relative of peanut. The ESTs produced in this study are a valuable resource for gene discovery, the characterization of new wild alleles, and for marker development. The ESTs were released in the [GenBank:EH041934 to EH048197].

  3. Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi.

    Science.gov (United States)

    Rey, Hebe Y; Faloci, Mirta; Medina, Ricardo; Dolce, Natalia; Mroginski, Luis; Engelmann, Florent

    2009-01-01

    A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23 percent moisture content (fresh weight basis). Explants were frozen using slow cooling (1 C per min from 25C to -30C followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 microM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53 percent and 56 percent of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, only 16 percent of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material. PMID:19946657

  4. Chlorophyll and carbohydrates in Arachis pintoi plants under influence of water regimes and nitrogen fertilization

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    Rita Manuele Porto Sales

    2013-06-01

    Full Text Available In this experiment the chlorophyll and carbohydrate contents of Arachis pintoi were evaluated to verify if the presence of nitrogen in the soil could contribute to the effectiveness of the establishment of this legume. The design was completely randomized, in a 4 × 4 factorial arrangement, with four N rates (0, 40, 80 and 120 kg ha-1 and four irrigation levels (25, 50, 75 and 100% of field capacity, with four replications. The biochemical evaluations of chlorophylls a and b and total chlorophyll and total soluble sugars, sucrose and starch were performed. The highest contents of chlorophyll a and b and total chlorophyll in leaves were found at the dose of 120 kg ha-1. The water regime of 25% of field capacity was responsible for the lowest content of reducing sugars and total soluble sugars in leaves, stolons and roots. In the roots, the sucrose contents were higher in these conditions, which can be associated with a slight tolerance of the plant to water stress. The water deficiency was responsible for the decrease of reducing sugars and total N in the whole plant and positively influenced the levels of chlorophyll and sugars in the stolon, promoting growth, especially of shoots, at the beginning of establishment.

  5. Steers performance in dwarf elephant grass pastures alone or mixed with Arachis pintoi.

    Science.gov (United States)

    Crestani, Steben; Ribeiro Filho, Henrique Mendonça Nunes; Miguel, Marcolino Frederico; de Almeida, Edison Xavier; Santos, Flávio Augusto Portela

    2013-08-01

    The inclusion of legumes in pasture reduces the need for mineral nitrogen applications and the pollution of groundwater; however, the agronomic and animal husbandry advantages with tropical legumes are still little known. The objective of this study was to quantify the effect of the use of forage peanut (Arachis pintoi cv. Amarillo) in dwarf elephant grass pastures (Pennisetum purpureum cv. BRS Kurumi) on forage intake and animal performance. The experimental treatments were dwarf elephant grass fertilized with 200 kg N/ha, and dwarf elephant grass mixed with forage peanut without mineral fertilizers. The animals used for the experiment were 12 Charolais steers (body weight (BW) = 288 ± 5.2 kg) divided into four lots (two per treatment). Pastures were managed under intermittent stocking with an herbage allowance of 5.4 kg dry matter of green leaves/100 kg BW. Dry matter intake (mean = 2.44% BW), the average daily gain (mean = 0.76 kg), and the stocking rate (mean = 3.8 AU/ha) were similar between the studied pastures, but decreased drastically in last grazing cycle with the same herbage allowance. The presence of peanut in dwarf elephant grass pastures was enough to sustain the stocking rate, but did not allow increasing forage intake and animal performance. PMID:23413007

  6. Characterization of rhizobia that nodulate Arachis pintoi by RAPD analysis Caracterização de rizóbios capazes de nodular Arachis pintoi via análise de "RAPD"

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    Patrícia Pereira Pinto

    2004-09-01

    Full Text Available The genetic relationships of 85 Arachis pintoi nodulating Rhizobium strains were determined using the random amplified polymorphic DNA (RAPD methods. The analysis included 75 strains isolated from Cerrado soils and 10 other ones of different origins. The results indicated that there is a high level of similarity between these strains and that geographic distribution may affect their phylogenetic relationship. In addition, the results allowed the selection of the most suitable primers for characterisation of these Rhizobium strains which will be useful for implementation of competitiveness studies in Cerrado soils.As relações genéticas de 85 estirpes de Rhizobium capazes de nodular Arachis pintoi foram determinadas usando o método de "RAPD" (Random Amplified Polymorphic DNA. As análises incluíram 75 estirpes isoladas de solos de Cerrado e 10 de diferentes origens. Os resultados indicaram que existe um alto grau de similaridade entre estas estirpes e que a distribuição geográfica pode afetar suas relações filogenéticas. Além disso, os resultados permitiram a seleção de "primers" mais adequados para a caracterização dessas estirpes de Rhizobium, os quais serão úteis para a implementação de estudos de competitividade nos solos de Cerrado.

  7. Nutritional Value of Manga Groundnut (Arachis hypogea and Characterization of Oil Extracted by Solvent

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    M. Dzondo-gadet

    2015-04-01

    Full Text Available In the present study, characteristics of seeds and oil extracted by soxhlet with hexane from Manga groundnut (Arachis hypogea are evaluated. The percentage composition of the Manga groundnut seeds is: lipid content (47.43±1.6%, protein content (32.64±0.26% and Total carbohydrates (17.56±1.33%, moisture (7.58±0.86 and ash (5.67±0.98. The major nutrients (mg/100 g of seeds found in the seeds are: potassium (2070.00±0.97 magnesium (350.00±0.84, calcium (45.00±0.67 and phosphate (672.19±0.97 and sodium (12.5±0.5. The physical properties of the oil extracts showed the state to be fluid at room temperature (25-30°C and the color to be pale-yellow or golden-yellow, in general. Chemical properties of the oil extracts, Acid value (Av 4.56 (oleic acid; Peroxide value (Pv (2.48 meq O2 /Kg, Saponification value (Sv (258.06 mg KOH/g and an Iodine value (Iv of 100.93±1.88 mg/100 were been evaluated. The oil contains high levels of oleic acid C18: 1 (53.18%, followed by linoleic acid C18: 2 (29.74% and palmitic acid C16: 0 (12.43%. There is slight fraction of stearic acid C18: 0 (1.52% and Behenic acid C 22: 0 (1.21%. The DSC cooling curves reveal that: cooling point = -12.9°C and cooling enthalpy = 49.64 J/g. The results of the present analytical study show that Manga groundnut oil could be useful as edible oils and for industrial applications.

  8. Assessment of Genetic Stability Among In Vitro Plants of Arachis retusa Using RAPD and AFLP Markers for Germplasm Preservation

    Institute of Scientific and Technical Information of China (English)

    Rachel Fatima Gagliardi; Luiz Ricardo Hanai; Georgia Pacheco; Carlos Alberto Oliveira; Leonardo Alves Carneiro; José Francisco Montenegro Valls; Elisabeth Mansur; Maria Lucia Carneiro Vieira

    2007-01-01

    Arachis retusa Krapov. et W. C. Gregory et Valls is endemic in the West-central region of Brazil, occurring in areas endangered by human actions. The establishment of in vitro preservation methods for wild species of Arachis isan alternative to seed banks for germplasm storage, multiplication and distribution. The risk of genetic changesinduced by tissue culture and the monitoring of the genetic stability of the biological material before, during andafter storage must be considered in the context of conservation. Random amplified polymorphic DNA (RAPD) andamplified fragment length polymorphism (AFLP) fingerprinting were used to evaluate the genetic stability of invitro plants originated from cotyledons and embryo axes of A. retusa. Cotyledons originated shoots through directorganogenesis and embryo axes displayed multishoot formation induced by 110 mmol/L and 8.8 mmol/L BAP,respectively. Ninety genomlc regions (loci) generated from RAPD and 372 from AFLP analyses were evaluated. Allamplified fragments detected by both techniques in plants derived from the two explant types were monomorphic.The results indicate that the recovered shoots are genetically stable at the assessed genomic regions.

  9. Avaliação do feno de Arachis pintoi utilizando o ensaio de digestibilidade in vivo

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    Ladeira Márcio Machado

    2002-01-01

    Full Text Available Utilizaram-se seis ovinos, sem raça definida, para avaliar o consumo e as digestibilidades aparentes totais da matéria seca (MS, matéria orgânica (MO, proteína bruta (PB, extrato etéreo (EE, carboidratos totais (CHO, fibra em detergente neutro (FDN, carboidratos não fibrosos (CNF, fibra em detergente ácido (FDA, celulose (CEL, hemicelulose (HCEL e energia do feno de Arachis pintoi. Também foi determinado o balanço de nitrogênio. Os animais foram colocados em gaiolas metabólicas e receberam apenas o feno de A. pintoi mais sal mineral como componentes da dieta. O Arachis pintoi foi colhido com aproximadamente 100 dias. O fornecimento do feno foi ad libitum, sendo a quantidade calculada para permitir sobras de 20%. O experimento teve 20 dias de duração, sendo 15 dias de adaptação e cinco dias para coletas de amostras do feno, sobras, fezes e urina. Foi utilizado o óxido crômico, em duas doses diárias de 1 g cada, como indicador externo para estimar a produção fecal. Os consumos de MS e MO do A. pintoi foram 90,17 e 85,67 g/kg0,75, respectivamente. Os teores de PB, nutrientes digestíveis totais (NDT e energia metabolizável (EM foram, respectivamente, 14,3%, 66,4% e 2,0 Mcal/kg MS. O balanço de nitrogênio (N foi de 12,1 g/dia e representou 40,2% de todo N consumido. As digestibilidades aparentes totais da MS, MO, PB, EE, CHO, FDN, CNF, FDA, CEL, HCEL e energia foram 64,4, 68,4, 70,0, 63,4, 68,2, 53,6, 93,3, 47,2, 62,8, 66,8 e 63,7%, respectivamente. O feno de Arachis pintoi apresentou consumo e digestibilidades dos nutrientes elevados para uma forrageira, permitindo assim fornecer nutrientes em quantidades suficientes para ganhos de peso satisfatórios, o que dá maior suporte para o uso dessa leguminosa na alimentação de ruminantes.

  10. Adaptação, produtividade e persistência de Arachis pintoi submetido a diferentes níveis de sombreamento Adaptation, productivity and persistence of Arachis pintoi under different levels of shading

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    Carlos Maurício Soares de Andrade

    1999-01-01

    Full Text Available Este experimento foi realizado com o objetivo de determinar o potencial forrageiro da leguminosa Arachis pintoi, submetida a 0, 30, 50 e 70% de sombreamento, em sistemas silvipastoris e como cobertura do solo em sistemas agroflorestais. O delineamento experimental usado foi o inteiramente casualizado, com quatro repetições. Realizou-se uma avaliação no final do período chuvoso e outra no final do período seco, usando as características altura e vigor de plantas, cobertura do solo e biomassa aérea, subterrânea e total. Os resultados mostraram que A. pintoi apresentou boa adaptação e persistência nos níveis de sombreamento estudados. A produtividade, apesar de ter diminuído com o aumento dos níveis de sombreamento, foi considerada adequada mesmo nos níveis mais altos. Concluiu-se que é possível usar esta leguminosa como cobertura do solo em sistemas agroflorestais e como forrageira em sistemas silvipastoris.The experiment was conducted to determine the forage potential of the Arachis pintoi submitted to 0, 30, 50 and 70% of shading, in silvopastoral systems and as ground cover in agroforestry systems. The experimental design was a completely randomized design with four replications. An evaluation was carried at the end of the rainy season and another at the end of the dry season, using the caracteristics height and plant vigor, ground cover, and total, above and below ground biomass. The results showed that A. pintoi presented good adaptation and persistence in the studied levels of shading. Although its productivity decreased with the increase of the levels of shading, it was considered adequate, even in the highest levels of shading. This indicates that it is possible to use this legume as ground cover in agroforestry systems and as forage in silvopastoral systems.

  11. Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa Influência de substratos e de pré-tratamentos in vitro na aclimatização ex vitro de Arachis retusa

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    Georgia Pacheco

    2006-01-01

    Full Text Available The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.O objetivo deste trabalho foi avaliar a influência de diferentes substratos e pré-tratamentos in vitro, na aclimatização de plantas in vitro de Arachis retusa. As plantas foram transferidas para Plantmax ou areia e adubadas com solução de Hoagland. Plantas mantidas em areia, com adubação ou sem adubação, apresentaram maiores taxas de sobrevivência. Para avaliação da influência de pré-tratamentos in vitro, segmentos de caule foram cultivados em meio MS suplementado com diferentes concentrações de sacarose. As maiores taxas de sobrevivência e desenvolvimento foram observadas em plantas cultivadas em sacarose a 1,5% e 3%. Depois de cinco meses, foram observadas a floração e a produção de frutos.

  12. Produção de novilhas de corte em pastagem de Coastcross-1 consorciada com Arachis pintoi com e sem adubação nitrogenada Beef heifer production in Coastcross-1 and Arachis pintoi mixed pasture with or without nitrogen fertilization

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    Wagner Paris

    2009-01-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar a massa de forragem (MF, a taxa de acúmulo diário (TAD, a oferta de forragem (OF, a taxa de lotação (TL, a porcentagem de Arachis pintoi (PAR, o ganho médio diário (GMD e o ganho por hectare (GPV/ha de novilhas de corte em pastejo de Coastcross-1 consorciada com Arachis pintoi. Os consórcios avaliados foram: CA0 = coastcross + Arachis pintoi sem adubação nitrogenada; CA100 = coastcross + Arachis pintoi com 100 kg de nitrogênio; CA200 = coastcross + Arachis pintoi com 200 kg de nitrogênio; e C200 = coastcross com 200 kg de nitrogênio, distribuídos em delineamento de blocos ao acaso, com duas repetições. O manejo do pasto foi o de lotação continua com carga animal variável utilizando-se novilhas mestiças com três animais-testes por consórcio. A massa de forragem nas pastagens de coastcross + Arachis pintoi adubadas com 0, 100 e 200 kg de nitrogênio e na pastagem de coastcross adubada com 200 kg de nitrogênio foi de 2.641, 2.431, 2.760 e 2.704 kg de MS/ha, respectivamente. A taxa de acúmulo diário foi semelhante (66,12 kg de MS/ha entre as pastagens; o verão foi a estação de maior produção, seguido da primavera, do outono, que não diferiram entre si, e do inverno (108,6; 71,1; 54,2; 30,6 kg de MS/ha, respectivamente. Na associação de coastcross + Arachis pintoi sem adubação nitrogenada, foram obtidas a maior oferta de forragem e a menor taxa de lotação (4,0 UA/ha. As maiores taxas de lotação e as menores ofertas de forragem foram observadas com a adubação nitrogenada. A porcentagem de Arachis pintoi foi maior na primavera e, na associação coastcross + Arachis pintoi sem adubação, as estimativas visuais foram sempre superiores às medidas, em virtude do baixo teor de matéria seca dessa leguminosa. O ganho médio diário foi maior no cultivo em consórcio e adubação com 200 kg de nitrogênio e na pastagem de coastcross em cultivo exclusivo com 200 kg

  13. Effect of peanut powder (Arachis hypogeae L., 1753) on zootechnic parameters and sex inversion in catfish Clarias gariepinus.

    Science.gov (United States)

    Jacques, Dougnon T; Elie, Montchowui; Messanvi, Gbeassor

    2015-01-01

    Benin is currently experiencing an overexploitation of piscatorial resources; this requires the research of endogenous means to increase the biomass of fish produced thanks to fish farming activities. The present study intends to improve the zootechnic performances and inverse the sex in catfish Clarias gariepinus. Therefore, 240 larvae obtained from artificial reproduction were used for this study. Three different feed were tested. The control feed (TO) was without peanut powder; contrary, the two experimental feeds were containing the powder at the rates of 10% (T1) and 20% (T2). The best growth of 94.51±27.14 g was recorded with the treatment T2 and 71.32±25.58 g from treatment T1 and finally 54.83±22.19 g from the control group. The sex inversion rate varied from 50% in the control group to 66.13% in lot 1 then 80.13% in lot 2. However, survival rates were low and varied from 26.25% for T2, to 30% in TO then 42.5% in T1. This study permitted to get better results about the zootechnic parameters and the sex inversion in Clarias gariepinus at incorporation rates of 10% and 20% of peanut powder "Arachis hypogeae." PMID:26571988

  14. Produção de forragem e desempenho animal em pastagens de coastcross consorciada ou não com Arachis pintoi, com e sem nitrogênio = Forage Production and Performance Animal in Coastcross Intercropping or not with Arachis pintoi, with or without Nitrogen

    Directory of Open Access Journals (Sweden)

    Ossival Lolato Ribeiro

    2008-10-01

    Full Text Available O estudo objetivou avaliar a produção de forragem e desempenho animal em pastagens de Coastcross + Arachis pintoi; Coastcross + Arachis pintoi com 100 kg ha-1 de N; Coastcross + Arachis pintoi com 200 kg ha-1 de N e Coastcross com 200 kg ha-1 de N, nas estações de inverno, primavera, verão e outono. Utilizou-se o delineamento experimentalem blocos ao acaso, com os tratamentos em parcelas subdivididas, com duas repetições. Foram avaliados: acúmulo de massa de forragem e acúmulo diário de massa de forragem, ganho médio diário (GMD, ganho de peso vivo por área e taxa de lotação. A utilização de Coastcross + 200 kg ha-1 de N e as melhores condições climáticas na primavera e verão favoreceram tanto o acúmulo de massa de forragem (26.764 kg ha-1 de MS quanto o acúmulo diário de massa de forragem (82 kg ha-1 por dia de MS. A utilização da associação entre Arachis pintoi + 200 kg ha-1 de N e Coastcross + 200 kg ha-1 de N, possibilitou o melhor desempenho animal, com GMD de 0,570 e 0,500 kg e taxa de lotação de 3,51 e 3,26 UA ha-1, respectivamente. A utilização de pastagem consorciada sem a associação com doses de nitrogênio (100 e 200 kg ha-1 não favoreceu (p > 0,05 o acúmulo de massa de forrageme a taxa de acúmulo diária. A utilização de 200 kg ha-1 de N, com e sem a leguminosa, proporcionou o melhor desempenho e lotação animal por área.The objective of this study was to evaluate dry matter production and animal performance in pastures of Coastcross + Arachis pintoi; Coastcross + Arachis pintoi with 100 kg ha-1 of N; Coastcross +Arachis pintoi with 200 kg ha-1 of N and Coastcross with 200 kg ha-1 of N, during winter, spring, summer and autumn. The experimental design was complete randomized blocks with split-plot parcels, with two repetitions. The study evaluated the accumulation of foragemass and dairy accumulation of forage mass, average daily gain (ADG, live weight gain and stocking rate. The used of

  15. Decomposition of Arachis pintoi and Hyparrhenia rufa litters in monoculture and intercropped systems under lowland soil Decomposição da serrapilheira de Arachis pintoi e Hyparrhenia rufa em sistemas de monocultura e consórcio sob solo de várzea

    OpenAIRE

    Christiane Abreu de Oliveira; Maria Rita Scotti Muzzi; Hortênsia Abrantes Purcino; Ivanildo Evódio Marriel; Nadja Maria Horta de Sá

    2003-01-01

    Tropical grasslands under lowland soils are generally underutilized and the litter of forage legumes may be used to recover these degraded pastures. The objective of this work was to study the dynamics of litter decomposition of Arachis pintoi (pinto peanut), Hyparrhenia rufa (thatching grass) and a mixture of both species in a lowland soil. These treatments were analyzed in three areas: grass monoculture, legume monoculture and legume intercropped with the grass during the dry and wet season...

  16. Influência do Fósforo, Micorriza e Nitrogênio no Conteúdo de Minerais de Brachiaria brizantha e Arachis pintoi Consorciados Effect of Phosphorus, Mycorrhizal and Nitrogen on Mineral Content of Brachiaria brizantha - Arachis pintoi Mixture

    Directory of Open Access Journals (Sweden)

    Ívina Paula Almeida dos Santos

    2002-01-01

    Full Text Available O experimento foi conduzido em casa de vegetação com o objetivo de avaliar a influência do fósforo (P, fungos micorrízicos arbusculares (FMA's e nitrogênio (N no acúmulo de minerais na MS da parte aérea de braquiária MG-4 (Brachiaria brizantha cv. MG-4 e amendoim forrageiro (Arachis pintoi cv. Amarillo consorciados, em solo de baixa fertilidade. O delineamento utilizado foi o inteiramente casualizado, num esquema fatorial 5x2x2, sendo cinco doses de P (25, 50, 75, 100 e 200 mg de P/kg de solo, dois tratamentos de inoculação do solo (inoculado e não com o FMA Glomus etunicatum e dois tratamentos de N (com e sem N em cobertura, com quatro repetições. Foi realizado o corte da parte aérea das plantas aos 60 dias após a germinação para a determinação das quantidades acumuladas de N, P, K, Ca, Mg e S na MS da parte aérea. As adubações fosfatada e, principalmente, a nitrogenada provocaram aumento no conteúdo de N, P, K, Ca, Mg e S na braquiária MG-4, não se verificando tal aumento com a micorrização. No amendoim forrageiro, observou-se redução destes minerais com a aplicação de N, ao passo que a micorrização resultou em aumento dos mesmos. Por outro lado, a adubação fosfatada provocou pequeno aumento no acúmulo de minerais na MS da parte aérea do amendoim forrageiro.This experiment was carried out in a greenhouse condition to study the effect of phosphorus, arbuscular mycorrhizal fungi and nitrogen on mineral accumulation in braquiaria MG-4 (Brachiaria brizantha cv. MG-4 above ground forage DM and peanut (Arachis pintoi cv. Amarillo mixture, in soil of low fertility. The experimental design was a completely randomized in a 5x2x2 factorial arrangement, with five P rates (25, 50, 75, 100 and 200 mg/kg of soil, two inoculations (inoculated and no inoculated and two levels of N (with and without N, with four replicates. The harvest of the above ground parts of plants was at 60 days after seed germination to determine

  17. Establecimiento de Arachis pintoi Krap. & Greg. como cobertura de suelo en plátano macho en Cárdenas, Tabasco

    OpenAIRE

    Ramos Hernández, Eder

    2012-01-01

    El experimento se realizó en Cárdenas, Tabasco en una plantación de plátano macho, en un suelo con textura franca, pH moderadamente ácido, contenido de materia orgánica y nitrógeno total bajo; con la finalidad de evaluar la repuesta al establecimiento de Arachis pintoi Krap. y Greg. como una cobertura viva, el cual se plantó a una distancia de 30 cm entre plantas y líneas. Se evaluaron tres porcentajes de sombra producida por la plantación de plátano macho (21, 45 y 50 %)....

  18. Evaluation of agronomic practices for the establishment of Pinto peanut (Arachis pintoi in native pastures of Mexico

    Directory of Open Access Journals (Sweden)

    E. Castillo-Gallegos

    2009-01-01

    Full Text Available Se realizaron tres experimentos en un clima cálido y húmedo para evaluar el establecimiento de Arachis pintoi CIAT 17434: 1 cero labranza y labranza reducida, con fertilización (P, K, Mg, Ca, Zn, Cu y B o sin fertilización; 2 control de la vegetación nativa con herbicida o chapeo, con quema o sin ella; y, con o sin fertilizante fosforado; y 3, siembra, por semilla, de tres accesiones CIAT de Arachis pintoi: 17434, 18744 y 18748, usando semilla en vainas. Los suelos de los sitios experimentales fueron Ultisoles, ácidos (Durustults, con un rango de pH de 4.1 a 5.2, y una capa impermeable situada entre 0 y 25 cm de profundidad. Se evaluó: número y altura de plantas, y suelo cubierto por la leguminosa, a 4, 8 y 12 semanas después de la siembra. En el experimento 1, se muestrearon cuadrantes dentro de cada parcela de tratamiento. En los experimentos 2 y 3 se empleó un diseño de bloques completos al azar con 3 bloques como repeticiones. Se realizaron análisis de varianza de acuerdo con el diseño experimental utilizado. En el experimento 1, el efecto principal de tratamientos sobre el número de plantas fue altamente significativo en las épocas de invierno, verano y sequía. El tiempo requerido para alcanzar un 50% de cobertura fue de 21 semanas para T2 (labranza mínima, sin fertilización en invierno; 21 semanas para T4 (cero labranza, sin fertilización en sequía; y 20 semanas para T1 (labranza mínima, con fertilización y T4 en el verano. En el experimento 2, el efecto principal del tiempo después de la siembra fue altamente significativo para todas las variables de respuesta. El tratamiento herbicida+quema produjo plantas con los tallos más altos (21.0±1.6 cm que el tratamiento de herbicida-sin quema (14.5±1.1 cm. La fertilización con P no incrementó la cobertura de la leguminosa. El tratamiento chapeo sin quema y sin fertilización resultó en una menor cobertura que el tratamiento herbicida+quema+fertilización. En el

  19. Arachis pintoi COMO COBERTURA DE SUELO EN CULTIVOS DE PLÁTANO MACHO (Musa AAB EN CÁRDENAS, TABASCO, MÉXICO

    Directory of Open Access Journals (Sweden)

    Eder Ramos Hernández

    2011-01-01

    Full Text Available El experimento se realizó en Cárdenas, Tabasco enuna plantación de plátano macho, en un suelo con texturafranca, pH moderadamente ácido, contenido de materiaorgánica y nitrógeno total bajo; con la finalidad de evaluar larepuesta al establecimiento de Arachis pintoi Krap. y Greg.como una cobertura viva. El diseño experimental fue bloquescompletos al azar con tres replicas. Se evaluaron tresporcentajes de sombra producida por el cultivo de plátanomacho (21, 45 y 50 %. El establecimiento de A. pintoi fuerealizado en forma directa utilizando material vegetativo de 20-25 cmde longitud, el cual se plantó a una distancia de 30 cm entreplantas y líneas. Estadísticamente el nivel con menor porcentajede sombra presentó los valores más altos en el índice desobrevivencia de estolones de Arachis (ISE a los 60 díasdespués de la siembra (dds y registró cobertura del suelo de94.2 % y crecimiento lateral de estolones 112.5 cm con alturapromedio de 6.2 cm (febrero-mayo 2009.

  20. Intensity of Ground Cover Crop Arachis pintoi, Rhizobium Inoculation and Phosphorus Application and Their Effects on Field Growth and Nutrient Status of Cocoa Plants

    Directory of Open Access Journals (Sweden)

    John Bako Baon

    2006-08-01

    Full Text Available Arachis pintoiis potentially as a cover crop for cocoa (Theobroma cacaoL. farm, however information regarding its effect on the growth of cocoa plants in the field is very limited. The objective of this experiment is to investigate the combined influence of ground cover crop A. pintoi, rhizobial bacterial inoculation and phosphorus (P fertilizer on the growth of cocoa in the field and nutrient status. This experiment laid out in split-split plot design consisted of three levels of cover crop (without, A. pintoiand Calopogonium caeruleum, two levels of rhizobium inoculation (not inoculated and inoculated and two levels of phosphorus application (no P added and P added. The results showed that in field condition the presence of A. pintoias cover crop did not affect the growth of cocoa. On the other hand, C. caeruleumas cover crop tended to restrict cocoa growth compared to A. pintoi. Application of P increased leaf number of cocoa plant. Biomass production of A. pintoiwas 40% higher than C. caeruleum. Soil organic carbon and nitrogen contents were not affected by ground cover crops, though higher value (0.235% N and 1.63% organic C was obtained from combined treatments of inoculation and P addition or neither inoculation nor P addition. In the case of no rhizobium inoculation, soil N content in cocoa farm with A. pintoicover crop was lower than that of without cover crop or with C. caeruleum. Cover crop increased plant N content when there was no inoculation, on the other hand rhizobium inoculation decreased N content of cocoa tissue. Tissue P content of cocoa plant was not influenced by A. Pintoicover crop or by rhizobium inoculation, except that the P tissue content of cocoa was 28% higher when the cover crop was C. caeruleumand inoculated. Key words : Arachis pintoi, Theobroma cacao, Calopogonium caeruleum, rhizobium, nitrogen, phosphorus.

  1. Características da pastagem e desempenho animal em uma consorciaçao de Panicum maximum Jacq CV. tanzânia e Arachis pintoi submetida a diferentes alturas de manejo

    OpenAIRE

    Perin, Rogério

    2012-01-01

    RESUMO No período de novembro de 2000 a março de 2001, uma pastagem de Tanzânia {Panicum maximum Jacq. cv. Tanzânia-1) consorciada com arachis (Arachis pintoi Krap. & Greg. cv. Amarillo), estabelecida em um sistema de integração lavoura-pecuária, foi avaliada sob pastejo contínuo na Região do Arenito Caiuá, noroeste do Paraná, visando aprofundar os conhecimentos referentes à relação planta-animal e assim obter subsídios para auxiliar na recomendação de um sistema de produção com potencial...

  2. Produção de forragem e desempenho animal em pastagens de coastcross consorciada ou não com Arachis pintoi, com e sem nitrogênio - DOI: 10.4025/actascianimsci.v30i4.6466 Forage Production and Performance Animal in Coastcross Intercropping or not with Arachis pintoi, with or without Nitrogen - DOI: 10.4025/actascianimsci.v30i4.6466

    Directory of Open Access Journals (Sweden)

    José Augusto Nogueira Gomes

    2009-03-01

    Full Text Available O estudo objetivou avaliar a produção de forragem e desempenho animal em pastagens de Coastcross + Arachis pintoi; Coastcross + Arachis pintoi com 100 kg ha-1 de N; Coastcross + Arachis pintoi com 200 kg ha-1 de N e Coastcross com 200 kg ha-1 de N, nas estações de inverno, primavera, verão e outono. Utilizou-se o delineamento experimental em blocos ao acaso, com os tratamentos em parcelas subdivididas, com duas repetições. Foram avaliados: acúmulo de massa de forragem e acúmulo diário de massa de forragem, ganho médio diário (GMD, ganho de peso vivo por área e taxa de lotação. A utilização de Coastcross + 200 kg ha-1 de N e as melhores condições climáticas na primavera e verão favoreceram tanto o acúmulo de massa de forragem (26.764 kg ha-1 de MS quanto o acúmulo diário de massa de forragem (82 kg ha-1 por dia de MS. A utilização da associação entre Arachis pintoi + 200 kg ha-1 de N e Coastcross + 200 kg ha-1 de N, possibilitou o melhor desempenho animal, com GMD de 0,570 e 0,500 kg e taxa de lotação de 3,51 e 3,26 UA ha-1, respectivamente. A utilização de pastagem consorciada sem a associação com doses de nitrogênio (100 e 200 kg ha-1 não favoreceu (p > 0,05 o acúmulo de massa de forragem e a taxa de acúmulo diária. A utilização de 200 kg ha-1 de N, com e sem a leguminosa, proporcionou o melhor desempenho e lotação animal por área.The objective of this study was to evaluate dry matter production and animal performance in pastures of Coastcross + Arachis pintoi; Coastcross + Arachis pintoi with 100 kg ha-1 of N; Coastcross + Arachis pintoi with 200 kg ha-1 of N and Coastcross with 200 kg ha-1 of N, during winter, spring, summer and autumn. The experimental design was complete randomized blocks with split-plot parcels, with two repetitions. The study evaluated the accumulation of forage mass and dairy accumulation of forage mass, average daily gain (ADG, live weight gain and stocking rate. The used of

  3. Uso de N-alcanos na estimativa da composição botânica em amostras com diferentes proporções de Brachiaria brizantha e Arachis pintoi Use of N-alkanes for estimations of botanical composition in samples with different proportions of Brachiaria brizantha and Arachis pintoi

    Directory of Open Access Journals (Sweden)

    Cristiano Côrtes

    2005-10-01

    Full Text Available Este trabalho foi conduzido para se determinar a composição de n-alcanos (C24 a C36 em diferentes proporções de dietas hipotéticas de Brachiaria brizantha Stapf. cv. Marandu e Arachis pintoi Koprov & Gregory. cv. Amarillo (0; 15; 30; 45; 60 e 100% de Arachis pintoi e identificar a combinação de alcanos que permite calcular a composição botânica de dietas com o menor valor residual (real menos o estimado. As forragens foram amostradas no verão e os n-alcanos extraídos pelo método de saponificação direta, sendo identificados e quantificados por meio de análise de cromatografia gasosa. O alcano C34 foi utilizado como padrão interno. As proporções de A. pintoi nas dietas foram estimadas pela minimização do z (soma dos quadrados dos desvios entre a proporção real dos alcanos analisados e as proporções pré-estabelecidas (tratamentos, utilizando-se a equação de Duncan et al. (1999. Observou-se que houve predomínio das cadeias carbônicas ímpares e que a concentração total de n-alcanos decresceu à medida que se aumentou a proporção de A. pintoi nos tratamentos. Estimativas acuradas da composição botânica de misturas de A. pintoi com B. brizantha foram obtidas utilizando-se os alcanos C29, C31, C33 e C35. O alcano C35 foi fundamental para a qualidade das estimativas. Os resultados indicaram o grande potencial da técnica para estudos com animais em pastejo.This trial was carried out to determine the composition of n-alkanes (C24 to C36 in hypothetical diets comprising of pure Brachiaria brizantha Stapf. cv. Marandu and Arachis pintoi Koprov & Gregory. cv. Amarillo and mixtures of these two spececies with 15%, 30%, 45%, or 60% of Arachis pintoi; it also intended to identify the combination of alkanes that allows to calculate the botanical composition of diets with the smallest residual value (real less estimated values. The forages were sampled in the summer. The n-alkanes were extracted for the direct saponification

  4. Determinação da fixação biológica de nitrogênio no amendoim forrageiro (Arachis spp. por intermédio da abundância natural de 15N Determination of biological nitrogen fixation by the forage groundnut (Arachis spp. using the 15N natural abundance technique

    Directory of Open Access Journals (Sweden)

    Cesar Heraclides Behling Miranda

    2003-12-01

    Full Text Available Quantificou-se a fixação biológica de nitrogênio (FBN em cinco acessos de Arachis pintoi (BRA31534, BRA31828, BRA31796, BRA15121 e BRA30333 e dois de A. repens (BRA31801 e BRA31861. Os mesmos foram estabelecidos em um solo Latosolo Vermelho Escuro sujeito a inundação estacional, sendo a FBN estimada segundo a técnica da abundância natural do isótopo 15N (d15N. Estolões dos acessos foram plantados em novembro de 1999, em parcelas de 2,0 m x 2,0 m, com quatro repetições, distribuídas em blocos ao acaso. A massa verde das plantas acima de cinco centímetros do solo foi colhida em janeiro de 2000 e seca em estufa a 65ºC até peso constante, sendo posteriormente pesada e moída para análise dos conteúdos em N e d15N, em espectrômetro de massa. Verificaram-se diferenças significativas entre os genótipos quanto à produção de matéria seca (MS e N total, sobressaindo-se BRA31534 e BRA31828, com produções de 4,2 t/ha e conteúdos totais de N de 102 e 110 kg/ha, respectivamente. Os acessos BRA30333 e BRA31861 produziram apenas 2,6 t de MS/ha, com 59 e 65 kg/ha de N total, respectivamente. As taxas de FBN dos acessos testados, medidas por comparação dos seus teores de d15N com os de plantas não fixadoras crescendo na mesma área, variaram de 36% (BRA15121 a 90% (BRA31828 do N total das plantas, equivalente a 26 e 99 kg de N/ha, respectivamente. Verificou-se correlação positiva e significativa (r = 0,92, pThe biological nitrogen fixation (BNF of five Arachis pintoi (BRA31534, BRA31828, BRA31796, BRA15121 E BRA30333 and two A. repens (BRA31801 e BRA31861 accessions, grown in a Dark Red Latosol prone to seasonal flooding was evaluated using the 15N natural abundance method (d15N. Stolons of each accession were planted in November 1999, in plots of 2.0 m by 2.0 m, with four replications allotted to randomized blocks. Plant mass above five cm was harvested in January 2000. There were significant differences among the tested

  5. Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, Arachis diogoi Confers Abiotic Stress Tolerance in Tobacco.

    Directory of Open Access Journals (Sweden)

    Akanksha Sharma

    Full Text Available Late embryogenesis abundant (LEA proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF corresponding to LEA gene, which got differentially expressed in wild peanut, Arachis diogoi against the late leaf spot pathogen, Phaeoisariopsis personata was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as AdLEA. AdLEA belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of AdLEA was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of AdLEA in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed AdLEA tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that AdLEA confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants.

  6. Ectopic Expression of an Atypical Hydrophobic Group 5 LEA Protein from Wild Peanut, Arachis diogoi Confers Abiotic Stress Tolerance in Tobacco.

    Science.gov (United States)

    Sharma, Akanksha; Kumar, Dilip; Kumar, Sumit; Rampuria, Sakshi; Reddy, Attipalli R; Kirti, Pulugurtha Bharadwaja

    2016-01-01

    Late embryogenesis abundant (LEA) proteins are a group of hydrophilic proteins, which accumulate in plants under varied stress conditions like drought, salinity, extreme temperatures and oxidative stress suggesting their role in the protection of plants against these stresses. A transcript derived fragment (TDF) corresponding to LEA gene, which got differentially expressed in wild peanut, Arachis diogoi against the late leaf spot pathogen, Phaeoisariopsis personata was used in this study. We have cloned its full length cDNA by RACE-PCR, which was designated as AdLEA. AdLEA belongs to the atypical Group 5C of LEA protein family as confirmed by sequence analysis. Group 5C LEA protein subfamily contains Pfam LEA_2 domain and is highly hydrophobic. In native conditions, expression of AdLEA was upregulated considerably upon hormonal and abiotic stress treatments emphasizing its role in abiotic stress tolerance. Subcellular localization studies showed that AdLEA protein is distributed in both nucleus and cytosol. Ectopic expression of AdLEA in tobacco resulted in enhanced tolerance of plants to dehydration, salinity and oxidative stress with the transgenic plants showing higher chlorophyll content and reduced lipid peroxidation as compared to wild type plants. Overexpressed AdLEA tobacco plants maintained better photosynthetic efficiency under drought conditions as demonstrated by chlorophyll fluorescence measurements. These plants showed enhanced transcript accumulation of some stress-responsive genes. Our study also elucidates that ROS levels were significantly reduced in leaves and stomatal guard cells of transgenic plants upon stress treatments. These results suggest that AdLEA confers multiple stress tolerance to plants, which make it a potential gene for genetic modification in plants. PMID:26938884

  7. Experiencias en el establecimiento de Arachis pintoi Krapov & W.C. Greg. como cobertura en cítricos de Veracruz, México

    Directory of Open Access Journals (Sweden)

    B. Valles

    2006-01-01

    Full Text Available Se realizaron dos experimentos para evaluar el establecimiento de Arachis pintoi (Ap como cobertera en cítricos; el primero, en limón Persa, y el segundo, en naranjo. En el primero se sembraron los ecotipos CIAT 17434, 18744 y 18748 en suelo rastreado, en surcos separados a un metro, y distancia de 50 cm entre plantas. En el segundo, se sembró Ap 17434 en suelo rastreado, escardado, u hoyado; plantando a 50 y 35 cm en surcos separados a 75 cm, con y sin P+K+Mg. La cobertura se evaluó a 4, 8, 12, 16, 20 y 24 semanas postsiembra, con la misma frecuencia en el segundo caso hasta 20 semanas. El diseño experimental fue para el primero completamente al azar; y el segundo, de bloques al azar, en parcelas subdivididas. Del primero, resultó que las semanas para alcanzar 50% y 100% de cobertura fueron 16 y 32, 12 y 24, y 13 y 26, para 17434, 18744 y 18748, respectivamente (P=0.0001. Para el segundo caso, los máximos valores de cobertura fueron en suelo rastreado, en rango de 53.5 a 87.5 %, según la densidad de siembra y fertilización. En los restantes tratamientos los valores fueron pobres (3.5 % a 33.7%. Del primer experimento, los ecotipos 18744 y 18748 se consideraron como los más promisorios en cuanto al tiempo necesario para cubrir totalmente el terreno. Para el segundo experimento, la preparación del terreno con pases de rastra garantizó el mejor establecimiento de la cobertura.

  8. AUTUMNAL FORAGE YIELD AND NUTRITIVE VALUE OF THE LEGUME ARACHIS RENDIMENTO E VALOR NUTRITIVO DA FORRAGEM OUTONAL DE AMENDOIM-FORRAGEIRO

    Directory of Open Access Journals (Sweden)

    Pedro Lima Monks

    2007-09-01

    Full Text Available

    Dry matter yield and nutritive value of forage le-gume Arachis    pintoi (Krap. & Greg. cv. Alqueire-1 (BRA 037036, was evaluated under different cutting mana-gement regimes and levels of P and K fertilization, in a yellow-red argisoil, at CAP-UFPEL, Capão do Leão, RS, Brazil during Spring-Summer and Fall. Cutting regimes compared were: no cutting, one, two, three, four and five cuttings, at 5 cm above ground. Fertilization levels con-sisted in supplying zero, 50 and 100% of requirements for P and K recommended by Brazilian Soil Science Society, for warm season perennial forage legumes. Fertilization treatments were alocated to main plots and cutting regi-mes to subplots, in a complete splitplot randomized block design, with three replications. Data of the following va-riables were submitted to analysis of variance and polino-mial regression: dry matter yield and quality of autumnal cutting, dry matter accumulation rate of autumnal cutting and total dry matter yield. If the purpose is the utilization of the forage during Autumn, 70% of the recommended phosphorus and potassium fertilization is sufficient to ob-tain maximum forage yield. However, if the objective are cuttings during the growing season (Spring-Summer and also in Autumn, it is necessary 100% of the recommended fertilization. The increase in number of cuttings during Spring-Summer decreases in the same proportion the fo-rage yield in Autumn. Forage nutritive value in Autumn is better when greater number of cuttings are made during Spring-Summer. Spring deferments also result in higher autumnal forage quality.

    KEY-WORDS: Cutting, fertilization, tropical forage.

    Num Argissolo vermelho amarelo eutrófico típi-co, do Centro Agropecuário da Palma, da UFPEL, Capão do Leão, RS,  foram avaliados os efeitos de cortes esti-vais e da adubação fosfatada e potássica sobre o rendi-mento e valor nutritivo da matéria seca (MS outonal de amendoim-forrageiro (Arachis

  9. Peanut, Cotton, and Corn Yield and Partial Net Income with Two Surface Drip Lateral Spacings

    Science.gov (United States)

    Surface drip irrigation laterals were spaced next to crop rows (0.91 m) and in alternate row middles (1.83 m) to document crop yield and partial net economic returns compared with non-irrigated peanut (Arachis hypogaea), cotton (Gossypium hirsutum), and corn (Zea mays). A drip irrigation system was ...

  10. Strategies in prevention of preharvest aflatoxin contamination in peanuts: Aflatoxin biosynthesis, genetics and genomics

    Science.gov (United States)

    The peanut (Arachis hypogaea L.), or groundnut, is an important crop economically and nutritionally worldwide. It is also one of the most susceptible host crops to Aspergillus flavus resulting in aflatoxin contamination. The reduction and elimination of aflatoxin contamination in pre-harvest and pos...

  11. Recombination is suppressed in an alien introgression in peanut harboring Rma, a dominant root-knot nematode resistance gene

    Science.gov (United States)

    Rma, a dominant root-knot nematode resistance gene introduced into tetraploid peanut (Arachis hypogaea) from a synthetic allotetraploid donor (TxAG-6), has been widely deployed in modern cultivars. The genomic location and borders of the alien chromosome segment introgressed from TxAG-6 into NemaTAM...

  12. Yield and economics of shallow subsurface drip irrigation (S3DI) and furrow diking

    Science.gov (United States)

    A shallow subsurface drip irrigation (S3DI) was installed yearly in conjunction with furrow diking to document yield and economic benefit of these techniques on peanut (Arachis hypogaea L.), cotton (Gossypium hirsutum L.), and corn (Zea mays L.). This research was conducted for three years from 2005...

  13. Weed Control and Peanut Tolerance with Ethalfluralin-Based Herbicide Systems

    OpenAIRE

    Grichar, W. J.; Dotray, P. A.

    2012-01-01

    Field studies were conducted from 2007 through 2009 to determine weed efficacy and peanut (Arachis hypogaea L.) response to herbicide systems that included ethalfluralin applied preplant incorporated. Control of devil's claw (Proboscidea louisianica (Mill.) Thellung), yellow nutsedge (Cyperus esculentus L.), Palmer amaranth (Amaranthus palmeri S. Wats.), and puncturevine (Tribulus terrestris L.) was most consistent with ethalfluralin followed by either imazapic or imazethapyr applied postemer...

  14. Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) accessions from the US mini core collection were analyzed for differentially expressed leaf proteins during reproductive stage under water-deficit stress. Accessions showing tolerant and susceptible responses to stress were selected based on a bioassay involving chloroph...

  15. Proteomics of water-deficit stress in US peanut mini core accesions

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) accessions from the US mini core collection were analyzed for differentially expressed leaf proteins during reproductive stage under water-deficit stress. Accessions showing tolerant and susceptible responses to stress were selected based on a bioassay involving chloroph...

  16. Generating a Natural Porcine Model of Gastrointestinal Food Allergy to Peanut

    Science.gov (United States)

    The peanut (Arachis hypogaea) is an extremely potent allergen and is one of the most life-threatening food sensitivities known. Peanuts cause the majority of food-related anaphylaxis in children, adolescents, and adults. There is no good animal model currently in place to study peanut allergies. Exp...

  17. Further investigations into the suitability of peanuts for biodiesel production

    Science.gov (United States)

    Field studies were conducted during 2007 at multiple locations to continue investigations into the suitability and practicality of peanut (Arachis hypogaea L.) as a biodiesel feedstock. An evaluation was conducted at Dawson, GA, to assess 24 peanut cultivars for performance under low input growing ...

  18. Modulation of ovomucoid-specific oral tolerance in mice fed plant extracts containing lectins

    DEFF Research Database (Denmark)

    Kjær, Tanja; Frøkiær, Hanne

    2002-01-01

    We investigated the effect of feeding extracts of four different legumes (red kidney bean (Phaseolus vulgaris), peanut (Arachis hypogaea), soyabean (Glycine max) and pea (Pisum sativum) on the specific immune response against a food protein. Mice were fed ovomucoid and the specific immune response...

  19. Physiological Response to Drought Stress at Different Stages in Peanut

    Science.gov (United States)

    Drought is a major factor in reducing productivity in peanut (Arachis hypogaea L.). The objectives of this study were to: 1) investigate the response patterns of relative water content (RWC), specific leaf area (SLA), and leaf dry mater content (LDMC) to drought stress at three stages of 30 60, and ...

  20. Relationship between root characteristics of peanut in hydroponics and pot studies

    Science.gov (United States)

    A large root system can be an important character for drought tolerance. Measuring root characteristics in soil medium is tedious, time consuming, and labor intensive. The objective of this study was to determine the association between root characteristics of peanut (Arachis hypogaea L.) Grown in...

  1. EST-based Microsatellite Marker Data Mining and Characterizing

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) is an important crop for oil production. In the recent years, molecular marker technologies have been widely applied to genetic diversity analysis, genetic mapping, molecular marker-assisted breeding, gene tagging and QTLs analysis. However, it is expensive, labor-intens...

  2. Endophytic associations and production of mycotoxins by the Aspergillus section Nigri species

    Science.gov (United States)

    The filamentous fungi of the Aspergillus section Nigri (black aspergilli) are considered plant pathogens of maize (Zea mays) and peanuts (Arachis hypogaea) where they can cause similar disease symptoms as Fusarium verticillioides, such as seedling blight. However, the main concern with black aspergi...

  3. The impact of a parasitic nematode Thripinema fuscum (Tylenchida: Allantonematidae) on the feeding behavior and vector competence of Frankliniella fusca (Thysanoptera: Thripidae)

    Science.gov (United States)

    Frankliniella fusca (Hinds) (Thysanoptera: Thripidae) is the predominant thrips species found inhabiting and reproducing in peanut (Arachis hypogaea L.) and is one of at least seven thrips species reported to transmit Tomato spotted wilt virus (TSWV). The entomogenous nematode Thripinema fuscum Tipp...

  4. Broiler litter ash and flue gas desulfurization gypsum effects on peanut yield and uptake of nutrients

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) is an important crop that requires large amounts of soluble calcium and phosphorus. Flue gas desulfurization gypsum (FGDG) and super phosphate (SP) have been used for calcium and phosphorus fertilizer for peanut. Broiler litter ash (BLA), a high phosphorus byproduct pr...

  5. Peanut Oil

    Science.gov (United States)

    Aceite de Cacahuete, Aceite de Maní, Arachide, Arachis hypogaea, Cacahouète, Cacahuète, Earth-Nut, Groundnuts, Huile d'Arachide, Huile de Cacahouète, Huile de Cacahuète, Monkey Nuts, Peanut, Peanuts.

  6. YIELD RESPONSE TO PEANUT ROW ORIENTATION AND SEEDING RATE WHEN IRRIGATED USING SDI

    Science.gov (United States)

    Peanut (Arachis hypogaea L.) are typically planted in a single or twin row orientation, however, research indicates that peanut planted at equidistance between rows and plants in alternating rows (diamond shape) and using the same planting rate can increase pod yield. A study was conducted to eval...

  7. Single and Twin Row Peanut Production within Narrow and Wide Strip Tillage Systems

    Science.gov (United States)

    Increased production costs and potential benefits of maintaining surface residue has renewed interest in conservation tillage systems for peanut (Arachis hypogaea L.) production. We initiated a study to determine remaining surface residue cover from rye (Secale cereale L.) or oat (Avena sativa L.)...

  8. Productivity and residual benefits of grain legumes to sorghum under semi-arid conditions in southwestern Zimbabwe

    NARCIS (Netherlands)

    Ncube, B.; Twomlow, S.J.; Wijk, van M.T.; Dimes, J.P.; Giller, K.E.

    2007-01-01

    The productivity and residual benefits of four grain legumes to sorghum (Sorghum bicolor) grown in rotation were measured under semi-arid conditions over three cropping seasons. Two varieties of each of the grain legumes; cowpea (Vigna unguiculata); groundnut (Arachis hypogaea); pigeon pea (Cajanus

  9. Technology and Application Effect of Interplanting Arachis Pintoi cv. Amarillo in Hilly Tea Garden%山地茶园套种平托花生的技术与应用效果

    Institute of Scientific and Technical Information of China (English)

    吴厚习; 陈天志

    2012-01-01

      The characteristics of arachis pintoi cv. Amarillo and the key technology of interplanting in hilly tea garden were elaborated in the paper. The effect of interplanting was also analyzed, so as to provide a good production mode for ecological tea garden construction in mountainous regions.%  本文阐述了平托花生的特征特性和山地茶园套种平托花生的关键技术,分析了茶园套种平托花生的应用效果,为丘陵山地生态茶园建设提供一种良好的生产模式。

  10. Uso de n-alcanos na estimativa da composição botânica da dieta em ovinos alimentados com diferentes proporções de Brachiaria decumbens Stapf e Arachis pintoi Koprov e Gregory Use of n-alkanes to estimate the dietary botanical composition in sheep fed different proportions of Brachiaria decumbens Stapf and Arachis pintoi Koprov and Gregory

    Directory of Open Access Journals (Sweden)

    Nelson Massaru Fukumoto

    2007-08-01

    Full Text Available Neste experimento objetivou-se avaliar o poder discriminatório dos n-alcanos para estimar com acurácia e precisão a composição botânica da dieta em ovinos alimentados com diferentes proporções de Arachis pintoi Koprov & Gregory cv. Amarillo (0, 15, 30, 45 e 60% e Brachiaria decumbens Stapf. Foram utilizados 20 ovinos em delineamento inteiramente casualizado, com período experimental de dez dias de adaptação à dieta e cinco dias de coleta de fezes. Nas amostras (compostas de fezes do período e nos fenos, foi analisada a concentração de n-alcanos. Para o cálculo da composição botânica, utilizou-se minimização da soma dos quadrados dos desvios, considerando as concentrações dos alcanos nos componentes da dieta e nas fezes. Para a escolha dos alcanos mais discriminatórios, foram utilizadas as análises multivariadas e as variáveis canônicas. As estimativas calculadas foram submetidas à análise de variância. As médias foram comparadas pelo teste t e as correções dos valores estimados em relação aos valores reais foram ajustadas em regressão linear. As variáveis canônicas indicaram que os alcanos C35, C33, C30, C31, C27, C29 e C36 são os de maior potencial discriminatório. O uso desses alcanos nos cálculos foi mais acurado e preciso para estimar a proporção de A. pintoi na dieta que o uso de apenas dois ou três alcanos com poder discriminatório. O melhor ajuste da regressão também foi encontrado para esses alcanos. O teste t para o intercepto da equação (a e o coeficiente de regressão (b indicaram que a = 0 e b = 1, comprovando que os valores estimados são equivalentes aos valores reais. As análises multivariadas mostraram-se ferramentas de grande importância na escolha dos n-alcanos nos cálculos nas estimativas.The objective of this experiment was to use n-alkane to estimate accurately and precisely the botanical composition of dietary forage in sheep fed different proportions of Arachis pintoi

  11. 2个多年生花生品种低温胁迫下活性氧代谢特性研究%Study on the characteristics of oxygen metabolism in two varieties of Prennial Arachis spp.

    Institute of Scientific and Technical Information of China (English)

    余高镜; 应朝阳; 黄毅斌; 柯碧南; 庄重光; 翁伯奇

    2006-01-01

    不同低温胁迫下对平托花生Arachis pintoi与蔓花生A.duranensis 2个多年生花生品种进行的叶片活性氧代谢与质膜特性进行研究.结果表明,A.duranensis品种无论在超氧化物岐化酶(SOD)、过氧化物酶(POD)还是过氧化氢酶(CAT)相对活性在低温胁迫下均不低于或显著高于A.pintoi,表现出相对较高的清除自由基能力;其质膜相对透性(RPP)增加幅度明显小于A.pintoi;丙二醛(MDA)相对含量增加率则表现出相反趋势;表明A.duranensis具有更强的抗寒性.

  12. Chemical similarity among domesticated and wild genotypes of peanut based on n-alkanes profiles

    Directory of Open Access Journals (Sweden)

    Renata Janaína Carvalho de Souza

    2010-11-01

    Full Text Available The objective of this work was to analyze the epicuticular n-alkane profile of domesticated and wild peanut genotypes. Foliar epicuticular n-alkanes of four Arachis hypogaea genotypes and two wild species - A. monticola and A. stenosperma - were analyzed by gas chromatography. Chemical relationships between them were evaluated using the Dice coefficient and UPGMA method. Two clusters were formed: one with four A. hypogaea genotypes and the other with the two wild species. There is more similarity between the BR1 and LIGO-PE06 genotypes and between the BRS 151 L-7 and BRS Havana genotypes.

  13. Influence of seaweed extract as an organic fertilizer on the growth and yield ofArachis hypogeaL. and their elemental composition usingSEM-EnergyDispersiveSpectroscopic analysis

    Institute of Scientific and Technical Information of China (English)

    G. Ganapathy Selvam; K. Sivakumar

    2014-01-01

    Objective:To investigate the effect ofSeaweedLiquidFertilizer(SLF) of the red seaweedHypnea musciformis(Wulfen)Lamouroux(H. musciformis). on the growth, biochemical and pigment characteristics ofArachis hypogea(A. hypogea).Methods:Experiments were conducted on ground nut to study the potential red alga ofH. musciformis as a biofertilizer.The seeds were sown in soil andSLF were added to soil bed in five different concentrations separately(1%,2%, 4%,6% and8% w/v).Results:The2% concentration of water extract showed better results of growth parameters, biochemical and pigments constitutions.Among the different concentrations ofSLF investigated, the plants that received with2%SLF showed maximum germination percentage, fresh weight, dry weight, root and shoot length, number of branches, leaf area, root nodules and content of total chlorophyll, chlorophyll a and b, protein, carbohydrate and lipid were observed at2% concentration ofSLF.The leaf of2%SLF treated A. hypogea has subjected toScanningElectronMicroscopy withEnergyDispersive spectroscopic analysis, it revealed that the presence of ten elements in the following order:Ca>P>N>Na>K>Mg>Mn>S>Fe>Zn in treated andCa>N>P>Na>Mg>Mn>K>Zn>S>Fe in control plant.The data generated from study reveal that SLF ofH. musciformiscould be used as foliar spray at low concentration of2% to maximize the growth and yield of A. hypogeaand also increase the number of stomata in the leaf.Conclusion:It is suggested that there are considerable gains to be made in increasing yield and stabilizing the yield in environments characterized by terminal requirement for organic and by shortening crop duration nutrient management appear promising.

  14. Microbial activity in soil cultivated with different summer legumes in coffee crop

    Directory of Open Access Journals (Sweden)

    Elcio Liborio Balota

    2011-02-01

    Full Text Available A field experiment was conducted for ten years in a sandy soil in the north part of the Paraná State, Brazil. The soil samples were collected at 0-10 cm depth, both under the coffee canopy and in the inter row space between the coffee plants, in the following treatments: Control, Leucaena leucocephala, Crotalaria spectabilis, Crotalaria breviflora, Mucuna pruriens, Mucuna deeringiana, Arachis hypogaea and Vigna unguiculata. The legume crops influenced the microbial activity, both under the coffee canopy and in the inter row space. The cultivation of Leucaena leucocephala increased the microbial biomass C, N and P. Although L. leucocephala and Arachis hypogaea provided higher microbial biomass, the qCO2 decreased by up to 50% under the coffee canopy and by about 25% in the inter row space. The soil microbial biomass was enriched in N and P due to green manure residue addition.

  15. Studies on the relationship between lectin binding carbohydrates and different strains of Leishmania from the New World

    OpenAIRE

    J. Schottelius; S. C. Gonçalves da Costa

    1982-01-01

    The culture forms of L. mexicana pifanoi (LRC L-90), L. mexicana mexicana (LRC L-94, M-379); L. braziliensis braziliensis (LRC L-77, L-1, M-2903, H-LSS) and L. mexicana amazonensis (H-JMMO, M-JOF, H-21, H-PLL,M-1696) were tested with the following lectins: Canavalia ensiformis, Ricinus communis-120, Axinella polypoides, Phaseolus vulgaris, Evonymus europaeus, lotus tetragonolobus, Dolichos biflorus, Aaptos papillata II, Laburnum alpinum, Ulex europaeus, Arachis hypogaea and Soja hispida. All ...

  16. Case Study: Trap Crop with Pheromone Traps for Suppressing Euschistus servus (Heteroptera: Pentatomidae) in Cotton

    OpenAIRE

    P. G. Tillman; Cottrell, T. E.

    2012-01-01

    The brown stink bug, Euschistus servus (Say), can disperse from source habitats, including corn, Zea mays L., and peanut, Arachis hypogaea L., into cotton, Gossypium hirsutum L. Therefore, a 2-year on-farm experiment was conducted to determine the effectiveness of a sorghum (Sorghum bicolor (L.) Moench spp. bicolor) trap crop, with or without Euschistus spp. pheromone traps, to suppress dispersal of this pest to cotton. In 2004, density of E. servus was lower in cotton fields with sorghum tra...

  17. Physiological and Biochemical Responses to Drought Stress and Subsequent Rehydration in the Symbiotic Association Peanut-Bradyrhizobium sp.

    OpenAIRE

    Ana Furlan; Analía Llanes; Virginia Luna; Stella Castro

    2012-01-01

    Drought stress is one of the most important environmental factors that regulate plant growth and development and limit its production. Peanut (Arachis hypogaea L.) is an agriculturally valuable plant with widespread distribution in the world serving as a subsistence food crop as well as a source of various food products. The aims of this work were to evaluate growth and nodulation as well as some physiological and biochemical stress indicators in response to drought stress and subsequent rehy...

  18. Towards prevention of allergy through an integrated multidisciplinary approach

    OpenAIRE

    Gilissen, L.J.W.J.; Heteren, van, J.T.; Meer, van der, D; Hall, R. D.

    2005-01-01

    Field experiments were conducted in Soroti District, north-eastern Uganda, an area with two rainy seasons per calendar year, the first one with long, reliable rains and a second one with short, less reliable rain. The trials were with sweet potato (Ipomoea batatas (L.) Lamk), groundnut (Arachis hypogaea L.) and maize (Zea mays L.) and aimed at collecting information on the incidence of millipede damage. Failure of sweet potato cuttings to establish caused by biotic stress varied from 4 to 33%...

  19. Productivity and residual benefits of grain legumes to sorghum under semi-arid conditions in southwestern Zimbabwe

    OpenAIRE

    Ncube, B.; Twomlow, S.J.; Wijk, van, M.J.; Dimes, J.P.; Giller, K.E.

    2007-01-01

    The productivity and residual benefits of four grain legumes to sorghum (Sorghum bicolor) grown in rotation were measured under semi-arid conditions over three cropping seasons. Two varieties of each of the grain legumes; cowpea (Vigna unguiculata); groundnut (Arachis hypogaea); pigeon pea (Cajanus cajan); Bambara groundnut (Vigna subterranea), and sorghum were grown during the first season. The same experiment was implemented three times in different, but adjacent fields that had similar soi...

  20. Monitoring of Aflatoxins in Peanuts

    OpenAIRE

    UÇKUN, Okşan; Var, Işıl

    2014-01-01

    Peanuts (Arachis hypogaea L.) are one of the most important oilseed crops and snack foods in the world Agro-food trade market. The major producers/exporters of peanuts are the United States, China, Argentina, Sudan, Senegal, and Brazil. Peanuts are a perishable commodity, easily spoiled by fungi. Aflatoxins are a group of natural compounds mainly produced by Aspergillus flavus and Aspergillus parasiticus. They have been found to be carcinogenic, teratogenic, and mutagenic to humans and animal...

  1. Groundnut improvement: use of genetic and genomic tools

    OpenAIRE

    Janila, Pasupuleti; Nigam, S. N.; Pandey, Manish K.; P. Nagesh; Rajeev K. Varshney

    2013-01-01

    Groundnut (Arachis hypogaea L.), a self-pollinated legume is an important crop cultivated in 24 million ha world over for extraction of edible oil and food uses. The kernels are rich in oil (48–50%) and protein (25–28%), and are source of several vitamins, minerals, antioxidants, biologically active polyphenols, flavonoids, and isoflavones. Improved varieties of groundnut with high yield potential were developed and released for cultivation world over. The improved varieties belong to differe...

  2. Allelopathic effects of ferulic, gallic, and vanillic acids on corn (Zea mays L.)

    OpenAIRE

    Abdaoui, Fatima El

    1991-01-01

    Studies on the activity of femlic, gallic, and vanillic acids on germination and growth of corn (Zea mays L.), radish (Raphanus sativus L.), and peanut (Arachis hypogaea L.) showed that the inhibitory effects of these acids were concentration and growth variable dependent. Ten days after treatment, significant reduction in percent germination of the three species occurred with higher phenolic acid treatments, except that gallic acid did not significantly inhibit peanut germi...

  3. Boron fertilization is a must to enhance peanut production in India

    OpenAIRE

    SINGH, AMRIT LAL Dr; Jat, R S; Misra, J B

    2009-01-01

    Boron (B) deficiency is a common factor causing low yield in large seeded peanut (Arachis hypogaea L.) in India. Field trials were conducted at Junagadh, Raichur, Vriddhachalam, Mainpuri, Durgapura and Kolasib in different soils to find out effectiveness and feasibility of applying commercial grade B sources i.e. Agricol, Chemiebor, Solubor, Borosol and Borax. The results indicated that application of B has pronounced influence on flowering and yield attributes, and all boron sources increase...

  4. Diversity and biological control of Sclerotium rolfsii, causal agent of stem rot of groundnut

    OpenAIRE

    Lê, N.C.

    2011-01-01

    Groundnut (Arachis hypogaea L.) is an economically important legume crop in Vietnam and many other countries worldwide. Stem rot disease, caused by the soil-borne fungus Sclerotium rolfsii Sacc., is a major yield limiting factor in groundnut cultivation. Current control methods mostly rely on the extensive use of fungicides and on cultural practices. Both methods are not always effective and repeated fungicide use can lead to resistance development in the pathogen population. To further impro...

  5. Effects of doubled CO2 concentration on leaf photosynthesis, transpiration and water use efficiency of eight crop species

    OpenAIRE

    Wang, Jian-Lin; WEN Xue-Fa; ZHAO Feng-Hua; FANG Quan-Xiao; YANG Xin-Min

    2012-01-01

    Aims Our objective was to elucidate the response of crop photosynthesis, transpiration and water use efficiency to atmospheric CO2 concentration. This has great significance to predicting crop productivity and water-demand changes under increasing atmospheric CO2 concentration.Methods The photosynthesis rate, transpiration rate and water use efficiency of eight crops (soybean (Glycine max), sweet potato (Ipomoea batatas), peanut (Arachis hypogaea), rice (Oryza sativa), cotton (Gossypium hirsu...

  6. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/
    Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

    OpenAIRE

    Débora Cristina Santiago; Édison Miglioranza; Romeu Munashi Endo; Nair Mieko Takaki Bellettini

    2005-01-01

    Seeds and seedlings of groundnut (Arachis hypogaea) cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were tra...

  7. Analysing retailers' buying behaviour and loyalty of branded adible groundnuts in the North West Province and Gauteng / Abraham Petrus Louw Lourens

    OpenAIRE

    Lourens, Abraham Petrus Louw

    2014-01-01

    South Africa is an exporter of groundnuts (Arachis Hypogaea). The domestic market reaps the spinoff advantage that export quality reaches store shelves, but unfortunately at a price that is directly linked to the price traders achieve on the export market. The local edible groundnut demand is relatively stable, but some processors saw a dramatic decline in the demand for groundnuts within the fast moving consumer goods (FMCG) industry. Businesses in the groundnut industry have ...

  8. Effects of plant essential oils and biocontrol agents on the growth of and mycotoxin production by Aspergillus spp. on groundnut

    OpenAIRE

    Alamene, Azawei

    2015-01-01

    Groundnut, Arachis hypogaea (L.), can be attacked by a range of pathogens, including Aspergillus species, which can cause accumulation of the mycotoxin aflatoxin. Although some success in controlling this pathogen has been achieved with application of fungicides, their use is not always feasible in developing nations like Nigeria. The aim of this study was, therefore, to evaluate naturally-occurring plant oils and BCAs with a past history of efficacy as alternatives to fungicides for reductio...

  9. Effects of Interactions of Auxin-Producing Bacteria and Bacterial-Feeding Nematodes on Regulation of Peanut Growths

    OpenAIRE

    Li Xu; Wensi Xu; Ying Jiang; Feng Hu; Huixin Li

    2015-01-01

    The influences of an IAA (indole-3-acetic acid)-producing bacterium (Bacillus megaterium) and two bacterial-feeding nematodes (Cephalobus sp. or Mesorhabditis sp.) on the growth of peanut (Arachis hypogaea L. cv. Haihua 1) after various durations of time were investigated in natural soils. The addition of bacteria and nematodes and incubation time all significantly affected plant growth, plant root growth, plant nutrient concentrations, soil nutrient concentrations, soil microorganisms and so...

  10. Effect of intercropping on nematodes in two small-scale sugarcane farming systems in South Africa

    OpenAIRE

    Berry, S. D.; Dana, P.; Spaull, V.W.; Cadet, Patrice

    2009-01-01

    Two trials were planted on sandy soils on small-scale grower farms to study the effect of intercropping on the nematode fauna, soil and plant fertility and sugarcane yield. Peanut (Arachis hypogaea) and sugar bean (Phaseolus limensis) were intercropped between the sugarcane rows in the first trial; velvet bean (Mucuna deeringiana) and sweet potato (Ipomoea batatas) were intercropped in the second trial. These practices were compared to a standard aldicarb (nematicide) treatment and an untreat...

  11. Niche-based assessment of contributions of legumes to the nitrogen economy of Western Kenya smallholder farms

    OpenAIRE

    Ojiem, J.O.; Vanlauwe, B.; Ridder, de, N.; Giller, K.E.

    2007-01-01

    Nitrogen (N) deficiency is a major constraint to the productivity of the African smallholder farming systems. Grain, green manure and forage legumes have the potential to improve the soil N fertility of smallholder farming systems through biological N-2-fixation. The N-2-fixation of bean (Phaseolus vulgaris), soyabean (Glycine max), groundnut (Arachis hypogaea), Lima bean (Phaseolus lunatus), lablab (Lablab purpureus), velvet bean (Mucuna pruriens), crotalaria (Crotalaria ochroleuca), jackbea...

  12. Amino Acid Profile of Some New Vartieties of Oil Seeds

    OpenAIRE

    Satish Ingale and S.K. Shrivastava

    2011-01-01

    There are large varieties of oil seeds and legumes in India, which are part of traditional food system but whose nutritional and economic values have not been completely determine and are far less exploited for both human and livestock utilization. The objective of this study was to evaluate Sunflower (Helianths annuus) LSF-11, Sunflower (Helianths annuus) LSF-8, Safflower (Carthamus tinctorius) PBNS-12, Safflower (Carthamus tinctorius) PBNS-40, and Ground nut (Arachis hypogaea) JL-24 seeds w...

  13. Host Status of Seven Weed Species and Their Effects on Ditylenchus destructor Infestation of Peanut

    OpenAIRE

    De Waele, D.; Jordaan, Elizabeth M.; Basson, Selmaré

    1990-01-01

    The host suitability to Ditylenchus destructor of seven common weed species in peanut (Arachis hypogaea) fields in South Africa was determined. Based on the number of nematodes per root unit, white goosefoot (Chenopodium album), feathertop chloris (Chloris virgata), purple nutsedge (Cyperus rotundus), jimson weed (Datura stramonium), goose grass (Eleusine indica), khaki weed (Tagetes minuta), and cocklebur (Xanthium strumarium) were poor hosts. Ditylenchus destructor survived on all weed spec...

  14. Histological Studies of Ditylenchus africanus Within Peanut Pods

    OpenAIRE

    Venter, C.; van Aswegen, G.; Meyer, A.J.; De Waele, D.

    1995-01-01

    Ditylenchus africanus entered the immature pegs and pods of peanut (Arachis hypogaea cv. Sellie) at the peg-connection and subsequently invaded the parenchymatous regions of the hull exocarp and endocarp, and eventually the seed testa. The nematode caused malformations of the cells of infected tissues, cell wall breakage, and cell collapse. The damage appeared to be due to enzymatic activity. In some testae the entire parenchyma region, which aids in protection of the seed, was destroyed. In ...

  15. Effect of Trichoderma harzianum biomass and Bradyrhizobium sp. strain NC 92 to control leaf blight disease of bambara groundnut (Vigna subterranea) caused by Rhizoctonia solani in the field

    OpenAIRE

    Mana Kanjanamaneesathian; Paranee Sawangsri; Ashara Pengnoo; Jira Suwanprasert

    2007-01-01

    Four hundred and sixty two strains of Trichoderma spp. were isolated from 23 soil samples in which groundnut (Arachis hypogaea L.) and bambara groundnut (Vigna subterranea L.) had been planted in Songkhla, Phattalung, Nakhon Si Thammarat, Narathiwat and Yala provinces. These fungi were tested against Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut, using dual culture technique on PDA medium. Among 462 isolates tested, 226 isolates had an ability to overgrow R. solani co...

  16. Molecular analysis of the chloroplast Cu/Zn-SOD gene (AhCSD2) in peanut

    Institute of Scientific and Technical Information of China (English)

    Xiurong Zhang; Qian Wan; Fengzhen Liu⁎; Kun Zhang; Aiqing Sun; Bing Luo; Li Sun; Yongshan Wan⁎⁎

    2015-01-01

    Superoxide dismutase (SOD, EC 1.15.1.1) plays a key role in response to drought stress, and differences in SOD activity changes among cultivars are important under drought conditions. We obtained the full-length DNA of the chloroplast Cu/Zn-SOD gene (AhCSD2) from 11 allotetraploid cultivars and 5 diploid wild species in peanut. BLAST search against the peanut genome showed that the AhCSD2 genes gCSD2-1 and gCSD2-2 are located at the tops of chromosome A03 (A genome) and B03 (B genome), respectively, and both contain 8 exons and 7 introns. Nucleotide sequence analyses indicated that gCSD2-2 sequences were identical among all the tested cultivars, while gCSD2-1 sequences showed allelic variations. The amino acid sequences deduced from gCSD2-1 and gCSD2-2 both contain a chloroplast transit peptide and are distinguished by 6 amino acid (aa) residue differences. The other 2 aa residue variations in the mature peptide regions give rise to three-dimensional structure changes of the protein deduced from the genes gCSD2-1 and gCSD2-2. Sequences analyses of cultivars and wild species showed that gCSD2-2 of Arachis hypogaea and gAipCSD2 (Arachis ipaensis) are identical, and despite the abundant polymorphic loci between gCSD2-1 of A. hypogaea and sequences from A genome wild species, the deduced amino acid sequence of AhCSD2-1 (A. hypogaea) is identical to that of AduCSD2 (Arachis duranensis), whereas AcoCSD2 (Arachis correntina) and AcaCSD2 (Arachis cardenasii) both have 2 aa differences in the transit peptide region compared with AhCSD2-1 (A. hypogaea). Based on the Peanut Genome Project, promoter prediction revealed many stress-related cis-acting elements within the potential promoter regions (pp-A and pp-B). pp-A contains more binding sites for drought-associated transcriptional factors than pp-B. We hypothesize that the marked changes in SOD activity in different cultivars under drought stress are tightly regulated by transcription factors through transcription and

  17. Sustainability of sunflower-based crop sequences in rainfed alfisols

    OpenAIRE

    Reddy B.N; Babu Sudhakara S.N.

    2003-01-01

    A field experiment was conducted at Hyderabad, India, for six years (1992 to 1997) to study the performance of rainy season sunflower at 50% (37.5:45:15kg N:P205:K20/ha), 100% (75:90:30kg N:P205:K20/ha) and 150% (112.5:135:45kg N:P205:K20/ha) recommended NPK in annual rotation with rainy season crops viz. sorghum (Sorghum bicolor (L) Moench), pigeonpea (Cajanus cajan (L) Millsp), groundnut (Arachis hypogaea. L), sunflower (Helianthus annuus L) and castor (Ricinus cummunis L). The study also d...

  18. Characterization of active miniature inverted-repeat transposable elements in the peanut genome

    OpenAIRE

    Shirasawa, Kenta; Hirakawa, Hideki; Tabata, Satoshi; Hasegawa, Makoto; Kiyoshima, Hiroyuki; Suzuki, Sigeru; Sasamoto, Sigemi; Watanabe, Akiko; Fujishiro, Tsunakazu; Isobe, Sachiko

    2012-01-01

    Miniature inverted-repeat transposable elements (MITEs), some of which are known as active non-autonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), AhMITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was i...

  19. Overexpression of Peanut Diacylglycerol Acyltransferase 2 in Escherichia coli

    OpenAIRE

    Zhenying Peng; Lan Li; Lianqun Yang; Bin Zhang; Gao Chen; Yuping Bi

    2013-01-01

    Diacylglycerol acyltransferase (DGAT) is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the peanut cultivar 'Luhua 14' using a homologous gene sequence method and rapid amplification of cDNA ends. To understand the role of AhDGAT2 in triacylglycerol biosynthesis, two AhDGAT2 nucleotide s...

  20. Indice botánico

    OpenAIRE

    2015-01-01

    Achiote: 91, 94, 198 Aguaje: 156 Aji: 59, 74, 171 Alamo: 171 Algodón: 94, 110, 124 Aliso: 52 Alnus acuminata: ver aliso Aloes: 171 Altramuce: 44 Arachis hypogaea: ver maní Arracacha esculenta: 69 Arveja: 74 Asaí: ver palmera Bactris gasipaes: 141 Balsa palo de: 180 Befaría ledifolia: 64 Bertholetia excelsa: 154 Bixa orellana: ver achiote Bombax: 178 Bombonax: ver palmera Barbasco: 199 Cabeza de negro: ver Humiro Cacao: 194, 217 Café: 175, 179, 217 Camote: 62, 69, 74, 97, 182, 192 Caña de azúc...

  1. Características agronômicas do amendoinzeiro sob irrigação com águas salinas em solo com biofertilizantes.

    OpenAIRE

    Sousa, Geocleber Gomes de; de Azevedo, Benito Moreira; Albuquerque, André Henrique Pinheiro; Mesquita, José Bruno Rego de; Viana, Thales Vinicius de Araújo

    2012-01-01

    Objetivou-se com esse trabalho avaliar o efeito da salinidade da água de irrigação nas características agronômicas do amendoinzeiro (Arachis hypogaea L.) cultivado em solo sem e com biofertilizantes. O experimento foi conduzido em estufa telada na Estação Agrometereológica, Campus do Pici, Fortaleza, CE. A semeadura foi feita em vasos utilizando-se, como substrato, um Argissolo Vermelho-Amarelo, com uma planta por vaso. O experimento obedeceu a um delineamento inteiramente casualizado, em esq...

  2. Características agronômicas do amendoinzeiro sob irrigação com águas salinas em solo com biofertilizantes = Agronomics Characteristicsof Peanuts under irrigation with saline water on soil with biofertilizers.

    OpenAIRE

    Geocleber Gomes de Sousa; Benito Moreira de Azevedo; André Henrique Pinheiro Albuquerque; José Bruno Rego de Mesquita; Thales Vinicius de Araújo Viana

    2012-01-01

    Objetivou-se com esse trabalho avaliar o efeito da salinidade da água de irrigação nas características agronômicas do amendoinzeiro (Arachis hypogaea L.) cultivado em solo sem e com biofertilizantes. O experimento foi conduzido em estufa telada na Estação Agrometereológica, Campus do Pici, Fortaleza, CE. A semeadura foi feita em vasos utilizando-se, como substrato, um Argissolo Vermelho-Amarelo, com uma planta por vaso. O experimento obedeceu a um delineamento inteiramente casualizado, em esq...

  3. Análise histoquímica foliar do amendoim: genótipos 'Tatu' e SO-909 Leaf histochemical analyses of peanut: genotypes 'Tatu' and SO-909

    OpenAIRE

    Renato Ferraz de Arruda Veiga; Graci Mirian Corso; Paulo Roberto Curi; João Paulo Feijão Teixeira

    1992-01-01

    Este trabalho teve por finalidade a análise histoquímica foliar de dois genótipos de amendoim (Arachis hypogaea L.), do tipo botânico Valência: SO-53 ('Tatu') e SO-909 (PI-259747), cuja literatura demonstra apresentarem respostas diferentes de resistência às principais moléstias fúngicas foliares do Brasil. Seções transversais das seguintes estruturas - pulvino, haste peciolar, raque, pulvínulo e folíolo - e seções paradérmicas de folíolos coletados em dois anos agrícolas consecutivos, foram ...

  4. Dicty_cDB: Contig-U12522-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Bigelowiella natans ubiquitin/ribo... 41 0.13 AY099118_1( AY099118 |pid:none) Chlorarachnion CCMP621 isolate... pub... 41 0.13 AY099119_1( AY099119 |pid:none) Chlorarachnion CCMP621 isolate pu...quitin... 38 0.84 DQ887067_1( DQ887067 |pid:none) Arachis hypogaea polyubiquitin ...aromyces japonicus mitochondrial DNA, ... 36 3.0 6 ( CZ527616 ) SRAA-aac50a06.b1 Strongyloides rat...AY286305_1( AY286305 |pid:none) Heterodera schachtii ubiquitin ext... 39 0.65 UQJNI( S17740 ;S14579) ubiquit

  5. Efeito do espaçamento entre fileiras de amendoim rasteiro na interferência de plantas daninhas na cultura Effect of peanut crop row spacing on weed interference in the culture

    OpenAIRE

    T.C.S. Dias; P.L.C.A Alves; M.C.M.D. Pavani; M. Nepomuceno

    2009-01-01

    Objetivou-se com este trabalho avaliar o efeito da redução do espaçamento entre fileiras nos períodos de interferência e na produtividade do amendoim rasteiro (Arachis hypogaea). O experimento foi instalado no município de Jaboticabal-SP. Os tratamentos constaram de dois espaçamentos entre fileiras (80 e 90 cm), divididos em dois grupos. No primeiro, as plantas daninhas foram controladas desde a emergência até 0 (interferência constante), 30, 45, 60, 82, 97 e 112 dias depois. No segundo, as p...

  6. Variations on metabolic activities of legume tissues through radiation in tissue culture

    International Nuclear Information System (INIS)

    Cell cultures from Arachis hypogaea L. cultivated in a modified medium developed by Murashige and Skoog (1962) showed vigorous qrowth after radiation treatment. Investigations on the effect of various sugars on the chlorophyll formation and growth of the irradiated tissues showed that sucrose was superior to maltose, glucose or fructose as a carbon source. Lactose and mannitol supported growth and development of chlorophyll to a less degree. On prolonging the cultures on a sugar free medium, the tissues failed to regain either growth or chlorophyll content. (author)

  7. Protoplast culture of some economically important plants: Studies on plant regeneration

    International Nuclear Information System (INIS)

    Protoplast culture was attempted for three economically important plants - Santalum album (Indian sandalwood tree), Tylophora indica (a medicinal plant) and Arachis hypogaea (peanut). In sandalwood, protoplasts were successfully isolated from various explants, such as leaf mesophylls, stem and hypocotyl calli and cell suspensions derived from a callus. In the cases of Tylophora callus tissues and Arachis, leaf mesophylls were used as source materials for isolating protoplasts. Different enzyme mixtures (pH 5.5-5.8), containing combinations of cellulase, hemicellulase, pectinase, macerozyme and driselase, were employed in various concentrations. Protoplasts of all three plants divided and yielded rapidly proliferating callus tissues. In sandalwood and Tylophora, plant regeneration was achieved through somatic embryogenesis/organogenesis. Protoplast-derived calli of peanut lacked the ability for plant regeneration. (author)

  8. Influence of temperature and diet on the development of Ulomoides dermestoides (Fairmaire, 1893 (Coleoptera, Tenebrionidae, Diaperinae

    Directory of Open Access Journals (Sweden)

    Renato C. Marinoni

    2001-06-01

    Full Text Available Ulomoides dermestoides (Fairmaire, 1893 develops in stored food products (peanuts, maize, oats, rice, sorghum, etc. and breeds successfully in the laboratory. To determine the best conditions for development, experiments were set up in different temperatures and diets, similar to storage conditions of peanuts (Arachis hypogaea L.. The higher viability of individuals and the shorter developmental time were observed in the diet composed of hulls and seeds of fruits at 21 and 24°C.Ulomoides dermestoides (Fairmaire, 1893 é um coleóptero que se desenvolve em produtos armazenados (amendoim, milho, aveia, arroz, sorgo, etc. e é facilmente criado em laboratório. Para avaliar as melhores condições de desenvolvimento foram estabelecidos experimentos em diferentes temperaturas e em dietas definidas por três diferentes condições de armazenamento de amendoim (Arachis hypogaea L.. A maior viabilidade de indivíduos e o menor tempo de desenvolvimento foram verificados na dieta constituída por frutos abertos (vagens e grãos e em temperaturas de 21 e 24°C. É discutida a possível influência da umidade relativa nos resultados.

  9. Omissão de macronutrientes em plantas de amendoim Deficiency of macronutrients in peanuts

    Directory of Open Access Journals (Sweden)

    Francisco Solano de Oliveira Rodrigues Filho

    1988-01-01

    Full Text Available Plantas de amendoim Arachis hypogaea L. 'Tatu' foram cultivadas em condições de casa de vegetação, em vasos de Mitscherlich contendo areia lavada e irrigados com solução nutritiva completa e com soluções nutritivas com a omissão de cada macronutriente (N, P, K, Ca, Mg e S. As plantas mostraram sintomas de deficiência de macronutrientes, com exceção do fósforo, na ausência de cada elemento, relacionados com os seus baixos teores e redução no crescimento, desenvolvimento e produção de matéria seca.Peanut plants (Arachis hypogaea L. were cultivated in washed sand, under greenhouse conditions, irrigated with a complete nutrient solution and nutrient solutions with absence of each macronutrient (N, P, K, Ca, Mg and S. Typical symptoms of macronutrient deficiencies were observed, except for phosphorus, related to the content of the element in the plant. The absence of macronutrients also influenced the plant dry matter production, development and height.

  10. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/ Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

    Directory of Open Access Journals (Sweden)

    Débora Cristina Santiago

    2005-06-01

    Full Text Available Seeds and seedlings of groundnut (Arachis hypogaea cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were transmitted by seeds and caused damping-off before and after seedling emergence.Sementes e plântulas de amendoim (Arachis hypogaea cv. Tatu foram avaliadas em condições de laboratório e casa de vegetação com o objetivo de detectar e identificar a presença de fungos patogênicos associados às sementes, além de determinar a patogenicidade e os danos por eles causados. Os fungos detectados foram Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. e Phoma sp.. Os fungos mais freqüentes foram A. niger e Penicillium sp.. Estes fungos foram transmitidos através das sementes e causaram tombamento de pré e pósemergência nas plântulas.

  11. 不同基因型对花生胚小叶植株再生的影响%Effect of Genotypes on Plant Regeneration of Embryonic Leaflet in Peanut

    Institute of Scientific and Technical Information of China (English)

    隋炯明; 乔利仙; 赵明霞; 徐丽娟; 王晶珊

    2012-01-01

    以不同类型18个花生品种成熟种子的胚小叶为外植体,对不定芽诱导及植株再生进行了研究,旨在为花生遗传转化和离体诱变等提供培养方法.将胚小叶外植体培养在添加1 mg/L NAA和6mg/LBAP的诱导培养基上,4周后转移到添加4 mg/L BAP的培养基上进行培养.结果表明,所有的供试品种芽点诱导率均高于60%,但5大类型间及品种间(62.2%~95.5%)存在显著差异,龙生型平均诱导率最高(87.2%),其次是珍珠豆型(81.5%),多粒型最低(63.1%).将形成芽点的外植体转移到添加4 mg/L BAP的培养基上后,部分外植体从芽点上分化出不定芽,继续培养不定芽伸长并再生植株.植株再生率也存在显著性差异,最高的是珍珠豆型(83.5%~97.1%),最低是多粒型(14.8%~22.0%).%To provide culture method for genetic transformation and tissue culture in vitro,efficient adventitious shoot induction and plant regeneration of embryonic leaflets among 18 peanut cultivars in 5 types were studied in this paper. The embryonic leaflets were cultured on MSB5 induction medium (Murashige and Skoog salts plus B5 vitamins) supplemented with 1 mg/L NAA and 6 mg/L BAP,and then transferred to the medium supplemented with 4 mg/L BAP. The result showed that the frequencies of bud point induction were different significantly in the cultivars tested that ranged 62.2%-95.5% as well as in 5 peanut types. The highest induction frequency was obtained in Arachis hypogaea var. hirsuta (87.2%),followed by Arachis hypogaea var. rulgaiis (81.5%),the lowest was Arachis hypogaea var. fastigiata (63.1%). When the explants with bud points were transferred to the medium supplemented with 4 mg/L BAP,some of the bud points developed and then regenerated plants. The regeneration rate of plant was also different,the cultivars in Arachis hypogaea var. rulgaris produced higher frequencies (83.5%-97.1%),while that of Arachis hypogaea var. fastigiata produced lower

  12. Taxation Policy in EMU - Julian Alworth and Giampaolo Arachi

    OpenAIRE

    Julian Alworth; Giampaolo Arachi

    2008-01-01

    This paper proposes to examine whether and how the introduction of the euro changed the impact of taxes on the economy or influenced the direction of tax policy. The paper surveys potential theoretical channels through which tax policy and exchange rate regimes are interrelated (capital mobility, strategic tax setting and trade policy). It is difficult to find strong empirical evidence of major, unique changes in the impact or determination of tax policy following the introduction of the euro...

  13. Molecular Responses of Groundnut (Arachis hypogea L. to Zinc Stress

    Directory of Open Access Journals (Sweden)

    A. John De Britto

    2013-08-01

    Full Text Available Heavy metals are important environmental pollutants and their toxicity is a problem of increasing significance for ecological, evolutionary and environmental reasons. The interference of germination related proteins by heavy metals has not been well documented at the proteomic and genomic level. In the current study, molecular responses of germinating groundnut seeds were investigated under Zinc stress. The SDS-PAGE showed the preliminary changes in the polypeptides patterns under Zinc stress. Restriction digestion banding pattern of EcoRI and Hind III enzymes showed distinct banding pattern in the treated plants.

  14. Development and characterization of highly polymorphic long TC repeat microsatellite markers for genetic analysis of peanut

    Directory of Open Access Journals (Sweden)

    Macedo Selma E

    2012-02-01

    Full Text Available Abstract Background Peanut (Arachis hypogaea L. is a crop of economic and social importance, mainly in tropical areas, and developing countries. Its molecular breeding has been hindered by a shortage of polymorphic genetic markers due to a very narrow genetic base. Microsatellites (SSRs are markers of choice in peanut because they are co-dominant, highly transferrable between species and easily applicable in the allotetraploid genome. In spite of substantial effort over the last few years by a number of research groups, the number of SSRs that are polymorphic for A. hypogaea is still limiting for routine application, creating the demand for the discovery of more markers polymorphic within cultivated germplasm. Findings A plasmid genomic library enriched for TC/AG repeats was constructed and 1401 clones sequenced. From the sequences obtained 146 primer pairs flanking mostly TC microsatellites were developed. The average number of repeat motifs amplified was 23. These 146 markers were characterized on 22 genotypes of cultivated peanut. In total 78 of the markers were polymorphic within cultivated germplasm. Most of those 78 markers were highly informative with an average of 5.4 alleles per locus being amplified. Average gene diversity index (GD was 0.6, and 66 markers showed a GD of more than 0.5. Genetic relationship analysis was performed and corroborated the current taxonomical classification of A. hypogaea subspecies and varieties. Conclusions The microsatellite markers described here are a useful resource for genetics and genomics in Arachis. In particular, the 66 markers that are highly polymorphic in cultivated peanut are a significant step towards routine genetic mapping and marker-assisted selection for the crop.

  15. Characterizing the Suitability of Selected Indigenous Soil Improving Legumes in a Humid Tropical Environment Using Shoot and Root Attributes

    Directory of Open Access Journals (Sweden)

    Anikwe, MAN.

    2003-01-01

    Full Text Available We studied the biomass accumulation, root length, nodulation, and chemical composition of roots and shoot of ten indigenous soil improving legumes in a humid tropical ecosystem with the view to selecting species for soil improvement programmes. Two cultivars of Vigna unguiculata, and one each of Glycine max, Arachis hypogaea, Crotararia ochroleuca, Cajanus cajan, Pueraria phaseoloides, Lablab purpureus, Mucuna pruriens and Vigna subterranea as treatments were planted in 20 kg pots containing soil from an Oxic paleustalf in Nigeria. The pots were arranged in randomized complete block layout with three replications in a greenhouse at IITA Ibadan, Nigeria. Results from the work show that M. pruriens and C. cajan produced the highest quantity of biomass. Root elongation was highest in M. pruriens whereas A. hypogaea produced the most root nodules with native rhizobia. The highest quantity of nodule dry weight was produced by A. hypogaea and P. phaseoloides whereas most of the legumes except G. max and P. phaseoloides had high and statistically comparable N content of between 2.36 and 3.34 mg.kg-1 N. The results show that the legumes have different root and shoot characteristics, which should be taken into consideration when selecting species for soil improvement programmes.

  16. Expression of antigen tf and galectin-3 in fibroadenoma

    Directory of Open Access Journals (Sweden)

    Gallegos Itandehui Belem

    2012-12-01

    Full Text Available Abstract Background Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors. Findings Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts. Conclusions Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

  17. Isolation and characterization of retrotransposons in wild and cultivated peanut species

    International Nuclear Information System (INIS)

    Retrotransposons are considered as a possible source for mutations due to their potential of spreading in the genome using a 'copy and paste'-like mechanism. We report about the isolation and characterization of a new Ty3-gypsy retrotransposon from allotetraploid peanut (Arachis hypogaea, 2n=4x=40) and its diploid ancestors A. duranensis (AA-genome, 2n=20) and A. ipaensis (BB-genome, 2n=20). We have identified two repetitive sequences, one showing high similarity at amino acid level to the reverse transcriptase of Athila-type retrotransposons, the other being AT-rich with no similarities to genebank sequences. Results from genome walking experiments gave first evidence that both sequences represented parts of the same Ty3-gypsy retrotransposon, the 5'-LTR (long terminal repeat)- and the pol (polyprotein)-region respectively. Fluorescent in situ hybridization (FISH) experiments showed that the element is dispersedly distributed on the chromosomes, absent from centromeres and telomeric regions, and more prominent in chromosomes of the A-genome. The element appeared to be moderately repetitive with copy numbers of about 430 (A. ipaensis), 1350 (A. duranensis),and 3000 (Arachis hypogaea) per haploid genome. Phylogenetic analysis of the deduced amino acid sequences of 80 isolated reverse transcriptase clones from the three species shed light on its evolution within the peanut species. The isolated sequences contained multiple stop-codons and so far, no evidence has been found that the element is still active. An outlook is given regarding finding new tools for the advancement of Arachis breeding programmes aimed at the transfer of resistance to biotic and abiotic stresses to peanut. (author)

  18. Isolation and Characterization of Retrotransposons in Wild and Cultivated Peanut Species

    International Nuclear Information System (INIS)

    Retrotransposons are considered a possible source for mutations due to their potential of spreading in the genome using a 'copy and paste'-like mechanism. Here we report on the isolation and characterization of a new Ty3 -gypsy retrotransposon from allotetraploid peanut (Arachis hypogaea, 2n=4x=40) and its diploid ancestors A. duranensis (AA-genome, 2n=20) and A. ipaensis (BB-genome, 2n=20). We have identified two repetitive sequences, one showing high similarity at the amino acid level to the reverse transcriptase of Athila-type retrotransposons, the other being AT-rich with no similarities to gene bank sequences. Results from genome walking experiments gave first evidence that both sequences represented parts of the same Ty3-gypsy retrotransposon, the 5'-LTR (long terminal repeat)- and the pol (polyprotein)-region respectively. Fluorescent in situ hybridization (FISH) experiments showed that the element is dispersedly distributed on the chromosomes, absent from centromeres and telomeric regions, and more prominent in chromosomes of the A-genome. The element appeared to be moderately repetitive with copy numbers of about 830 (A. ipaensis ), 2,600 (A. duranensis), and 3,000 (Arachis hypogaea) per haploid genome. Phylogenetic analysis of the deduced amino acid sequences of 80 isolated reverse transcriptase clones from the three species shed light on its evolution within the peanut species. The isolated sequences contained multiple stop-codons and so far, no evidence has been found that the element is still active. An outlook is given regarding finding new tools for the advancement of Arachis breeding programs aimed at the transfer of resistance to biotic and abiotic stresses to peanut. (author)

  19. LAS RAZAS DE MANÍ DE BOLIVIA The peanut landraces from Bolivia

    Directory of Open Access Journals (Sweden)

    Antonio Krapovickas

    2010-07-01

    Full Text Available

    Bolivia se destaca como probable lugar de origen del maní cultivado y un centro de variación único en el mundo, lo que señala a Bolivia como una importante fuente de materiales para el mejoramiento de este cultivo de importancia mundial. En este estudio para maní se diferencian para Bolivia 62 razas de maní cultivado de las cuales, 42 pertenecen a Arachis hypogaea L subsp. hypogaea var. hypogaea, 17 a A. hypogaea susbp. fastigiata Waldron var. fastigiata, 1 a A. hypogaea susbp. fastigiata var. vulgaris y 2 a A. hypogaea subsp. fastigiata var. peruviana Krapov. & W. C. Gregory. Bolivia constituye un centro de variación independiente pues sus razas son exclusivas de su territorio, salvo muy pocas excepciones. Bolivia como unidad, se caracteriza por las razas "Crema", "Colorado San Simón", "Bayo americano", "Overo" y "Overo carenado", que se cultivan en todo el país. Se pueden delimitar algunas regiones de mayor diversidad: la región de los Yungas de La Paz, donde se coleccionaron 11 razas de las cuales 3 son exclusivas y la región montañosa de Santa Cruz y Cochabamba, con 18 razas de las cuales 6 son exclusivas. Estas dos regiones pertenecen a la cuenca del Amazonas. Una
    tercera región se encuentra en el Departamento de Tarija, perteneciente a la cuenca del Plata, donde se coleccionaron 14 razas, de las cuales 2 son exclusivas. Todas estas razas pertenecen a la var. hypogaea. La subsp. fastigiata, presenta un centro de variación muy notable en la cuenca del río Beni, donde en un área muy pequeña se coleccionaron 10 razas, de las cuales 9 son exclusivas. Se destaca Bolivia como un centro de variación único en el mundo y la gran variabilidad del maní señala a Bolivia como una importante fuente de posibilidades para el mejoramiento de este cultivo
  20. Interrelationships between Bacillus sp. CHEP5 and Bradyrhizobium sp. SEMIA6144 in the induced systemic resistance against Sclerotium rolfsii and symbiosis on peanut plants

    Indian Academy of Sciences (India)

    Marí­a Soledad Figueredo; María Laura Tonellie; Tania Taurian; Jorge Angelini; Fernando Ibañez; Lucio Valetti; Vanina Muñoz; Marí­a Soledad Anzuay; Liliana Ludueña; Adriana Fabra

    2014-12-01

    Plant-growth-promoting bacteria are often used to enhance crop yield and for biological control of phytopathogens. Bacillus sp. CHEP5 is a biocontrol agent that induces systemic resistance (ISR) in Arachis hypogaea L. (peanut) against Sclerotium rolfsii, the causal agent of root and stem wilt. In this work, the effect of the co-inoculation of Bacillus sp. CHEP5 and the peanut nodulating strain Bradyrhizobium sp. SEMIA 6144 was studied on induction of both systemic resistance and nodulation processes. Bradyrhizobium sp. SEMIA 6144 did not affect the ability of Bacillus sp. CHEP5 to protect peanut plants from S. rolfsii by ISR and the priming in challenged-plants, as evidenced by an increment in phenylalanine ammonia-lyase enzyme activity. Additionally, the capacity of Bradyrhizobium sp. SEMIA 6144 to induce nodule formation in pathogen-challenged plants was improved by the presence of Bacillus sp. CHEP5.

  1. Evaluation of yield and N2 fixation of mutant lines of groundnut in Malaysia

    International Nuclear Information System (INIS)

    The 15N-dilution technique was used to evaluate N2 fixation in groundnut (Arachis hypogaea L.) in three field trials of cultivars Matjan and V-13 (parents), their selected mutant lines, and a other local and foreign genotypes. Matjan mutant MJ/40/42 consistently produced the highest pod yields, at above 4 t ha-1, 14-22% higher yields than the parent. In contrast, none of the V-13 mutants had consistently better yields than the parent. The mutant lines did not show consistent agronomic performance from year to year. Total dry matter yield did not correlate with pod yield, and pod yield did not correlate with amount of N fixed

  2. Kazusa Marker DataBase: a database for genomics, genetics, and molecular breeding in plants.

    Science.gov (United States)

    Shirasawa, Kenta; Isobe, Sachiko; Tabata, Satoshi; Hirakawa, Hideki

    2014-09-01

    In order to provide useful genomic information for agronomical plants, we have established a database, the Kazusa Marker DataBase (http://marker.kazusa.or.jp). This database includes information on DNA markers, e.g., SSR and SNP markers, genetic linkage maps, and physical maps, that were developed at the Kazusa DNA Research Institute. Keyword searches for the markers, sequence data used for marker development, and experimental conditions are also available through this database. Currently, 10 plant species have been targeted: tomato (Solanum lycopersicum), pepper (Capsicum annuum), strawberry (Fragaria × ananassa), radish (Raphanus sativus), Lotus japonicus, soybean (Glycine max), peanut (Arachis hypogaea), red clover (Trifolium pratense), white clover (Trifolium repens), and eucalyptus (Eucalyptus camaldulensis). In addition, the number of plant species registered in this database will be increased as our research progresses. The Kazusa Marker DataBase will be a useful tool for both basic and applied sciences, such as genomics, genetics, and molecular breeding in crops. PMID:25320561

  3. Inoculação com Rhizobium e aplicação de nitrogênio em amendoim Comparison among Rhizobium strains inoculations and nitrogen applications on peanut, in field conditions

    OpenAIRE

    Antonio Roberto Giardini; Eli Sidney Lopes; Angelo Savy Filho; André Martin Louis Neptune

    1985-01-01

    Existe, nas nossas condições, uma população autóctone de Rhizobium capaz de nodular o amendoim (Arachis hypogaea L.), mas pouco se sabe da contribuição do nitrogênio fixado para esta planta. Foram conduzidos dois ensaios no campo, em solo de baixa fertilidade, um no período "da seca" e outro no "das águas", comparando o crescimento e a produção de plantas de amendoim inoculado com Rhizobium selecionado, com o de plantas noduladas pela população autóctone, adubadas ou não com nitrogênio. A nod...

  4. Systemic and oral immunogenicity of hemagglutinin protein of rinderpest virus expressed by transgenic peanut plants in a mouse model

    International Nuclear Information System (INIS)

    Rinderpest causes a devastating disease, often fatal, in wild and domestic ruminants. It has been eradicated successfully using a live, attenuated vaccine from most part of the world leaving a few foci of disease in parts of Africa, the Middle East, and South Asia. We have developed transgenic peanut (Arachis hypogaea L.) plants expressing hemagglutinin (H) protein of rinderpest virus (RPV), which is antigenically authentic. In this work, we have evaluated the immunogenicity of peanut-expressed H protein using mouse model, administered parenterally as well as orally. Intraperitoneal immunization of mice with the transgenic peanut extract elicited antibody response specific to H. These antibodies neutralized virus infectivity in vitro. Oral immunization of mice with transgenic peanut induced H-specific serum IgG and IgA antibodies. The systemic and oral immunogenicity of plant-derived H in absence of any adjuvant indicates the potential of edible vaccine for rinderpest

  5. Chemical composition of volatile fraction of pakistani peanut and its

    International Nuclear Information System (INIS)

    Arachis hypogaea Linn (peanut or groundnut) is an economically important crop. Thousands of peanut cultivars are grown in the world and studies have been carried out on these but, to the best of our knowledge, no salient work has been done on Pakistani cultivar so far. Here, we report the chemical composition of volatile fraction of Pakistani cultivar of peanut and its anti radical activities using 1,I-Diphenyl-2-picrylhydrazyl radical (DPPH) scavenging and phospho molybdenum complex (PC) method. This study revealed that Pakistani cultivar exhibited an almost equal antioxidant potential to that of standard, butylated hydroxytoluene (BHT), which was obvious from their IC/sub 50/ values. The IC/sub 50/ value of peanut extract was found to be 13.42 +- 26 macro L/mL, relative to butylated hydroxytoluene, having 12.1 +- 0.92 macro/mL. (author)

  6. Regeneration of M1 plants from 'de-embryonated' cotyledons to modify diplontic selection

    International Nuclear Information System (INIS)

    Peanut (Arachis hypogaea L.) cotyledons, after excision of the embryonal axis, were cultured in enamel trays lined with moist cotton wool. The cotyledons turned green and produced callus, roots or adventitious shoots in the basal region from where the embryonal axis was removed. Application of benzyladenine enhanced the frequency of adventitious shoot formation. When transferred to the field, the cotyledons developed into nearly normal-looking plants which produced pods and seeds. Such cotyledonary plants raised from unirradiated control and seeds exposed to 20 kR of gamma rays were progeny tested in the M2 generation. No mutations were found in 153 cotyledonary plant progenies of control. Sixteen per cent (13 out of 80) of the cotyledonary plant progenies raised from seeds exposed to gamma rays segregated for mutation. This was lower than the number of seed-raised progenies that segregated for mutation. (author)

  7. Enhanced Soil Carbon Storage under Agroforestry and Afforestation in Subtropical China

    Directory of Open Access Journals (Sweden)

    Guibin Wang

    2015-07-01

    Full Text Available Soil carbon (C in three Ginkgo (Ginkgo biloba L. agroforestry systems, afforestation (Ginkgo alone; G, and an agricultural cropping system were compared over a five-year period. The agroforestry systems were Ginkgo + Wheat (Triticum aestivum L. + Peanut (Arachis hypogaea L.; GWP; Ginkgo + Mulberry (Morus alba L.; GM; and Ginkgo + Rapa (Brassica napus L. + Peanut (GRP. The agricultural system consisted of wheat and peanut (WP. Total soil carbon (TSC, soil organic (SOC and inorganic carbon (SIC, and the pools of five SOC chemical fractions were measured. TSC and SOC were always lower under WP than the G-based planting systems, and TSC in the latter increased significantly across years in the top 20 cm. Stocks of SIC under WP were significantly greater than the G-based systems, whereas SOC fractions tended to be lower. Most fractions increased across years but not in WP.

  8. Effect of end of season water deficit on phenolic compounds in peanut genotypes with different levels of resistance to drought.

    Science.gov (United States)

    Aninbon, C; Jogloy, S; Vorasoot, N; Patanothai, A; Nuchadomrong, S; Senawong, T

    2016-04-01

    Terminal drought reduces pod yield and affected the phenolic content of leaves, stems and seed of peanut (Arachis hypogaea L.). The aim of this study was to investigate the effects of end of season water deficit on phenolic content in drought tolerant and sensitive genotypes of peanuts. Five peanut genotypes were planted under two water regimes, field capacity and 1/3 available water. Phenolic content was analyzed in seeds, leaves, and stems. The results revealed that terminal drought decreased phenolic content in seeds of both tolerant and sensitive genotypes. Phenolic content in leaves and stems increased under terminal drought stress in both years. This study provides basic information on changes in phenolic content in several parts of peanut plants when subjected to drought stress. Future studies to define the effect of terminal drought stress on specific phenolic compounds and antioxidant properties in peanut are warranted. PMID:26593473

  9. Dicty_cDB: Contig-U11314-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available KBrB046M04,... 38 0.23 7 ( X74411 ) Candida parapsilosis complete mitochondrial genome. 32 0.24 7 ( DQ376035 ) Candida para...seille... 54 1e-05 AE017196_930( AE017196 |pid:none) Wolbachia endosymbiont of Drosop... 54 1e-05 (A7Z1S6) RecName: Full=Cad...EQU... 48 1.0 1 ( DX516963 ) 763_5_14177545_16654_43716_017 Arachis hypogaea m...... 48 1.0 1 ( DY493477 ) sh2P0009K01_F.ab1 adult sheep fracture callus 7d ... 48 1.0 1 ( DY150553 ) 001105BEMN...ter hamburgensis X14, c... 44 0.021 EU180848_11( EU180848 |pid:none) Boechera divaricarpa clone BAC45b... 4

  10. Effet structurant de la plante hôte chez la bruche de l'arachide, Caryedon serratus (Olivier, 1790 (Coleoptera : Bruchidae

    Directory of Open Access Journals (Sweden)

    Sembène, M.

    2012-01-01

    Full Text Available Structuring effect of the host plant in the groundnut bruchid, Caryedon serratus (Olivier, 1790 (Coleoptera: Bruchidae. Twenty-six samples of the groundnut seed-beetle which were reared from pods of five different host plants (Arachis hypogaea L., Bauhinia rufescens Lam., Cassia sieberiana DC., Piliostigma reticulatum (DC. Hochst. and Tamarindus indica L. in four localities of Senegal were compared using electrophoresis based on six loci of four enzymatic systems. The population structure of Caryedon serratus Olivier was analysed using Weir and Cockerham's estimator of Wright's F-statistics. θ value (0.235 and the dendrogram of Rogers'genetic distances revealed a high degree of genetic differentiation between host plants. Genetic analysis without C. sieberiana samples indicated that populations form host races which are partially isolated according to their host plants (θ = 0.035. Geographical distances between localities are not decisive for genetic structuration of C. serratus populations from a given host plant.

  11. Dicty_cDB: Contig-U04900-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available n C23G10.2; &U39851_11(U39... 57 3e-07 AY426768_8( AY426768 |pid:none) Streptomyces clavuligerus putative....017346_153( AE017346 |pid:none) Cryptococcus neoformans var. neo... 50 3e-05 AM270210_16( AM270210 |pid:none) Aspergillus niger..._1( DQ889600 |pid:none) Arachis hypogaea clone XX20 perchl... 65 1e-09 CP000724_2230( CP000724 |pid:none) Alkaliphilus metalliredige... |pid:none) Acaryochloris marina MBIC11017,... 59 9e-08 CP001115_52( CP001115 |pid:none) Deinococcus deserti...nella sp. MR-4, complete g... 57 3e-07 CP001114_2218( CP001114 |pid:none) Deinococcus deserti VCD115, com...

  12. Effect of crop residue harvest on long-term crop yield, soil erosion, and carbon balance: tradeoffs for a sustainable bioenergy feedstock

    Energy Technology Data Exchange (ETDEWEB)

    Gregg, Jay S.; Izaurralde, Roberto C.

    2010-08-26

    Agricultural residues are a potential feedstock for bioenergy production, if residue harvest can be done sustainably. The relationship between crop residue harvest, soil erosion, crop yield and carbon balance was modeled with the Erosion Productivity Impact Calculator/ Environment Policy Integrated Climate (EPIC) using a factorial design. Four crop rotations (winter wheat [Triticum aestivum (L.)] – sunflower [Helianthus annuus]; spring wheat [Triticum aestivum (L.)] – canola [Brassica napus]; corn [Zea mays L.] – soybean [Glycine max (L.) Merr.]; and cotton [Gossypium hirsutum] – peanut [Arachis hypogaea]) were simulated at four US locations each, under different topographies (0-10% slope), and management practices [crop residue removal rates (0-75%), conservation practices (no till, contour cropping, strip cropping, terracing)].

  13. Dicty_cDB: Contig-U09710-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Oryza sativa (japonica cultivar-gr... 70 2e-10 AY027437_1( AY027437 |pid:none) Arachis hypogaea serine/thre...cw*fw*sl*rfi*r*rscn*sikind*ikrn*ri*kripnyechkietcxxflwxxig tkimxgxgklxxrxxxsxxg**xxx--- ---KWEIKVCDFGLSRFXTG...e kinase k... 56 2e-06 AB006554_1( AB006554 |pid:none) Hydra magnipapillata mRNA ...e-06 BC073081_1( BC073081 |pid:none) Xenopus laevis receptor-interactin... 56 3e-06 (Q9TTY2) RecName: Full=P...ia integrifolia subsp. infla... 54 1e-05 (Q9ERK0) RecName: Full=Receptor-interacting serine/threonine-pro...

  14. Herbicidas na nodulação e na atividade da nitrogenase em cultura de amendoim Influence of herbicides in nodulation and nitrogenase activities in peanuts crop

    Directory of Open Access Journals (Sweden)

    Maria do Sarmo S. Soares Novo

    1990-01-01

    Full Text Available Com o objetivo de estudar os efeitos de trifluralin, metolachlor e linufon, aplicados, respectivamente, em pré-plantio-incorporado, pré-emergência e em pós-emergência, sobre o peso de matéria seca e número de nódulos e sobre a atividade da nitrogenase, foi conduzido um experimento de campo, em cultura de amendoim (Arachis hypogaea cv. Tatu plantada em rotação com cana-de-açúcar. Os resultados das avaliações feitas aos 30, 50 e 80 dias após a germinação mostraram não haver diferença entre tratamentos inoculados e não inoculados. Linuron foi o único herbicida prejudicial à nodulação, tendo afetado o número e o peso da matéria seca de nódulos, nas diferentes amostragens, e inibido a fixação do nitrogênio (estimada pela técnica da redução do acetileno. Não foi observado efeito prejudicial dos herbicidas metolachtor e trifluralin, na nodulação e na atividade da nitrogenase.A field investigation was carried out to determine the influence of herbicides on peanuts (Arachis hypogaea cv. Tatu Bradyrhizobium sp. symbiosis. The herbicides studied were trifluralin, metolachlor and linuron, applied, respectively, in pre-planted-incorporated, pre-emergence and post-emergence. The results evaluated 30, 50 and 80 days after germination, showed no difference between inoculated and non inoculated treatment. Linuron was the only herbicide that caused damage to nodulation, affecting the number and dry weight nodules and also inhibiting the nitrogen fixation (determined by nitrogenase activity. Metolachlor and trifluralin showed no damage effects on nodulation and in the nitrogenase activity.

  15. Storage protein profiles in Spanish and runner market type peanuts and potential markers

    Directory of Open Access Journals (Sweden)

    Luo M

    2006-10-01

    Full Text Available Abstract Background Proteomic analysis has proven to be the most powerful method for describing plant species and lines, and for identification of proteins in complex mixtures. The strength of this method resides in high resolving power of two-dimensional electrophoresis (2-DE, coupled with highly sensitive mass spectrometry (MS, and sequence homology search. By using this method, we might find polymorphic markers to differentiate peanut subspecies. Results Total proteins extracted from seeds of 12 different genotypes of cultivated peanut (Arachis hypogaea L., comprised of runner market (A. hypogaea ssp. hypogaea and Spanish-bunch market type (A. hypogaea ssp. fastigiata, were separated by electrophoresis on both one- and two-dimensional SDS-PAGE gels. The protein profiles were similar on one-dimensional gels for all tested peanut genotypes. However, peanut genotype A13 lacked one major band with a molecular weight of about 35 kDa. There was one minor band with a molecular weight of 27 kDa that was present in all runner peanut genotypes and the Spanish-derivatives (GT-YY7, GT-YY20, and GT-YY79. The Spanish-derivatives have a runner-type peanut in their pedigrees. The 35 kDa protein in A13 and the 27 kDa protein in runner-type peanut genotypes were confirmed on the 2-D SDS-PAGE gels. Among more than 150 main protein spots on the 2-D gels, four protein spots that were individually marked as spots 1–4 showed polymorphic patterns between runner-type and Spanish-bunch peanuts. Spot 1 (ca. 22.5 kDa, pI 3.9 and spot 2 (ca. 23.5 kDa, pI 5.7 were observed in all Spanish-bunch genotypes, but were not found in runner types. In contrast, spot 3 (ca. 23 kDa, pI 6.6 and spot 4 (ca. 22 kDa, pI 6.8 were present in all runner peanut genotypes but not in Spanish-bunch genotypes. These four protein spots were sequenced. Based on the internal and N-terminal amino acid sequences, these proteins are isoforms (iso-Ara h3 of each other, are iso-allergens and may be

  16. Studies on the relationship between lectin binding carbohydrates and different strains of Leishmania from the New World

    Directory of Open Access Journals (Sweden)

    J. Schottelius

    1982-03-01

    Full Text Available The culture forms of L. mexicana pifanoi (LRC L-90, L. mexicana mexicana (LRC L-94, M-379; L. braziliensis braziliensis (LRC L-77, L-1, M-2903, H-LSS and L. mexicana amazonensis (H-JMMO, M-JOF, H-21, H-PLL,M-1696 were tested with the following lectins: Canavalia ensiformis, Ricinus communis-120, Axinella polypoides, Phaseolus vulgaris, Evonymus europaeus, lotus tetragonolobus, Dolichos biflorus, Aaptos papillata II, Laburnum alpinum, Ulex europaeus, Arachis hypogaea and Soja hispida. All examined strains of Leishmania were agglutinated by C. ensiformis, R. communis-120 and A. popypoides. No agglutination reactions were observed with P. vulgaris, D.biflorus, A. papillata II, E. europaeus and L. tetragonolobus. Only L. m. pifanoi and the L. m. amazonensis strains H-JMMO and MJOF showed agglutination reactions with S. hispida, U. europaeus, L. alpinum and A. hypogaea, while L. m. mexicana (LRC L-94; M-379 strains, L. b. braziliensis H. LSS, LRC L-77; L-1; M-2903 and the L. m. amazonensis strains, H-PLL, H-21, M-1696 showed no agglutination reactions with these four lectins.

  17. Characterization of active miniature inverted-repeat transposable elements in the peanut genome.

    Science.gov (United States)

    Shirasawa, Kenta; Hirakawa, Hideki; Tabata, Satoshi; Hasegawa, Makoto; Kiyoshima, Hiroyuki; Suzuki, Sigeru; Sasamoto, Sigemi; Watanabe, Akiko; Fujishiro, Tsunakazu; Isobe, Sachiko

    2012-05-01

    Miniature inverted-repeat transposable elements (MITEs), some of which are known as active nonautonomous DNA transposons, are found in the genomes of plants and animals. In peanut (Arachis hypogaea), Ah-MITE1 has been identified in a gene for fatty-acid desaturase, and possessed excision activity. However, the AhMITE1 distribution and frequency of excision have not been determined for the peanut genome. In order to characterize AhMITE1s, their genomic diversity and transposition ability was investigated. Southern blot analysis indicated high AhMITE1 copy number in the genomes of A. hypogaea, A. magna and A. monticola, but not in A. duranensis. A total of 504 AhMITE1s were identified from the MITE-enriched genomic libraries of A. hypogaea. The representative AhMITE1s exhibited a mean length of 205.5 bp and a GC content of 30.1%, with AT-rich, 9 bp target site duplications and 25 bp terminal inverted repeats. PCR analyses were performed using primer pairs designed against both flanking sequences of each AhMITE1. These analyses detected polymorphisms at 169 out of 411 insertional loci in the four peanut lines. In subsequent analyses of 60 gamma-irradiated mutant lines, four Ah-MITE1 excisions showed footprint mutations at the 109 loci tested. This study characterizes AhMITE1s in peanut and discusses their use as DNA markers and mutagens for the genetics, genomics and breeding of peanut and its relatives. PMID:22294450

  18. Genotypic diversity among rhizospheric bacteria of three legumes assessed by cultivation-dependent and cultivation-independent techniques.

    Science.gov (United States)

    Pongsilp, Neelawan; Nimnoi, Pongrawee; Lumyong, Saisamorn

    2012-02-01

    The genotypic diversity of rhizospheric bacteria of 3 legumes including Vigna radiata, Arachis hypogaea and Acacia mangium was compared by using cultivation-dependent and cultivation-independent methods. For cultivation-dependent method, Random amplified polymorphic DNA (RAPD) profiles revealed that the bacterial genetic diversity of V. radiata and A. mangium rhizospheres was higher than that of A. hypogaea rhizosphere. For cultivation-independent method, Denaturing gradient gel electrophoresis (DGGE) profiles of PCR-amplified 16S rRNA genes revealed the difference in bacterial community and diversity of rhizospheres collected from 3 legumes. The ribotype richness which indicates species diversity, was highest in V. radiata rhizosphere, followed by A. hypogaea and A. mangium rhizospheres, respectively. Three kinds of media were used to cultivate different target groups of bacteria. The result indicates that the communities of cultivable bacteria in 3 rhizospheres recovered from nutrient agar (NA) medium were mostly different from each other, while Bradyrhizobium selective medium (BJSM) and nitrogen-free medium shaped the communities of cultivable bacteria. Nine isolates grown on BJSM were identified by 16S rRNA gene sequence analysis. These isolates were very closely related (with 96% to 99% identities) to either one of the three groups including Cupriavidus-Ralstonia group, Bacillus group and Bradyrhizobium-Bosea-Afipia group. The rhizospheres were also examined for their enzymatic patterns. Of 19 enzymes tested, 3 rhizospheres were distinguishable by the presence or the absence of leucine acrylamidase and acid phosphatase. The selected cultivable bacteria recovered from NA varied in their abilities to produce indole-acetic acid and ammnonia. The resistance to 10 antibiotics was indistinguishable among bacteria isolated from different rhizospheres. PMID:22806857

  19. Global transcriptome analysis of two wild relatives of peanut under drought and fungi infection

    Directory of Open Access Journals (Sweden)

    Guimarães Patricia M

    2012-08-01

    Full Text Available Abstract Background Cultivated peanut (Arachis hypogaea is one of the most widely grown grain legumes in the world, being valued for its high protein and unsaturated oil contents. Worldwide, the major constraints to peanut production are drought and fungal diseases. Wild Arachis species, which are exclusively South American in origin, have high genetic diversity and have been selected during evolution in a range of environments and biotic stresses, constituting a rich source of allele diversity. Arachis stenosperma harbors resistances to a number of pests, including fungal diseases, whilst A. duranensis has shown improved tolerance to water limited stress. In this study, these species were used for the creation of an extensive databank of wild Arachis transcripts under stress which will constitute a rich source for gene discovery and molecular markers development. Results Transcriptome analysis of cDNA collections from A. stenosperma challenged with Cercosporidium personatum (Berk. and M.A. Curtis Deighton, and A. duranensis submitted to gradual water limited stress was conducted using 454 GS FLX Titanium generating a total of 7.4 x 105 raw sequence reads covering 211 Mbp of both genomes. High quality reads were assembled to 7,723 contigs for A. stenosperma and 12,792 for A. duranensis and functional annotation indicated that 95% of the contigs in both species could be appointed to GO annotation categories. A number of transcription factors families and defense related genes were identified in both species. Additionally, the expression of five A. stenosperma Resistance Gene Analogs (RGAs and four retrotransposon (FIDEL-related sequences were analyzed by qRT-PCR. This data set was used to design a total of 2,325 EST-SSRs, of which a subset of 584 amplified in both species and 214 were shown to be polymorphic using ePCR. Conclusions This study comprises one of the largest unigene dataset for wild Arachis species and will help to elucidate genes

  20. Aplikasi Pupuk Kandang Kotoran Ayam pada Tanaman Kacang Tanah (Arachis Hypogeae L.

    Directory of Open Access Journals (Sweden)

    Neni Marlina

    2015-09-01

    Full Text Available Pupuk kandang kotoran ayam diharapkan dapat memperbaiki sifat fisik, kimia dan biologi tanah, sehingga dapat menyuburkan tanah dan membantu dalam menyumbangkan unsur hara yang dapat digunakan dalam meningkatkan hasil kacang tanah.  Penelitian ini bertujuan untuk mendapatkan takaran pupuk kandang kotoran ayam yang tepat dalam meningkatkan produksi tanaman kacang tanah. Penelitian ini telah dilaksanakan di kebun petani di Desa Payakabung Kecamatan Indralaya Utara Kabupaten Ogan Ilir dari bulan Januari sampai dengan April  2014. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK dengan tiga perlakuan dan delapan kelompok, sehingga berjumlah 24 petak penelitian dan setiap petak diambil 10 tanaman sebagai sampel .  Perlakuannya adalah takaran pupuk kandang kotoran ayam 5, 10 dan 15 ton ha-1.  Hasil penelitian menunjukkan bahwa takaran pupuk kandang kotoran ayam sebanyak 10 ton ha-1 memberikan pertumbuhan dan produksi terbaik dengan ditunjukkan produksi per petak sebesar 2,73 kg petak-1.Poultry manure is expected to improve soil physical, chemical and biological properties. It can improve soil fertility and help in nutrients contribution that can be used to increase the yield of peanut. This study aimed to get the right dose of poultry manure fertilizer in increasing the production of ground peanut plants. This research was conducted in farmyard in the North Indralaya Payakabung District of Ogan Ilir from January to April 2014. The design used in this study was a randomized block design with three treatments and eight groups, thus consisting 24 research plots and each plot was taken as a sample of 10 plants. The treatments of poultry manure fertilizer rate 5, 10 and 15 ton ha-1. The results showed that poultry manure fertilizer rate as much as 10 tons ha-1 gave the best growth and production of 2.73 kg per plot.

  1. STUDY ON THE PERFORMANCE OF GROUNDNUT (ARACHIS HYPOGEA L. GENOTYPES FOR SEED YIELD AND QUALITY TRAITS

    Directory of Open Access Journals (Sweden)

    Chaitanya R. KOKKIRIPATI

    2015-12-01

    Full Text Available The present investigation was carried out at Department of Genetics and Plant Breeding, Sam Higginbottom Institute of Agriculture, Technology & Sciences (SHIATS, Allahabad, Uttar Pradesh during kharif 2014. The experimental material consisted of 11 Groundnut genotypes along with one check (IND-156. The genotypes were sown at Field Experimentation Centre in three replications adopting randomized block design to evaluate seed yield and quality traits. Analysis of variance revealed that the presence of considerable variation among the genotypes for all the characters studied. On the basis of mean performance, genotype ICG 14127 revealed better performance in primary branches/ plant, pods per plant, pod yield/plant, seed yield/plant and ICG 14482 showed better performance in kernel yield q/ha, oil content, oil yield while ICG 188 showed higher protein content.

  2. Fish oil versus arachis oil food supplementation in relation to pregnancy duration in rats

    DEFF Research Database (Denmark)

    Olsen, S.F.; Hansen, Harald S.; Jensen, B.

    1990-01-01

    fatty acids and lower levels of n-6 fatty acids in maternal and fetal tissues at delivery. FO-rats had a longer average gestational age than AO-rats (p <0.01), primarily due to a high proportion of AO-rats with short gestations. Birthweights of FO-rats were lower than those of AO-rats (P <0.05), which...... may be related to the lower maternal weight gain during pregnancy and the lower food consumption observed in FO-rats (p <0.01). Litter size and occurrence of stillborn pups were not significantly different in the groups (p > 0.1). The findings are compatible with the hypothesis that gestational age is...

  3. Quality characteristics of oil extracted from gamma irradiated peanut (Arachis hypogea L.)

    International Nuclear Information System (INIS)

    The effect of gamma radiation and storage on the characteristics of oil extracted from peanut seeds has been investigated in this study. Peanut seeds were undergone gamma irradiation process with the doses of 1, 2 and 3 kGy. The changes in chemical and physical attributes were observed immediately after irradiation and after 12 months of storage. The data obtained from the experiments showed that irradiation process had no effect on the chemical and physical qualities such as, fatty acid composition, peroxide value, iodine value specification number, TBA value and color of oil extracted from peanut seeds. On the contrary, the peroxide, acidity and TBA values of the peanut oil were decreased due to storage time. - Highlights: • Peanut seeds were undergone gamma irradiation (1, 2 and 3 kGy). • The change in physicochemical properties of peanut oil was determined. • Gamma irradiation process had no effect on physicochemical properties of peanut oil. • The peroxide, acidity and TBA values of the peanut oil were decreased due to storage time

  4. Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut

    Directory of Open Access Journals (Sweden)

    Guo Yufang

    2012-11-01

    Full Text Available Abstract Background Cultivated peanut or groundnut (Arachis hypogaea L. is an important oilseed crop with an allotetraploid genome (AABB, 2n = 4x = 40. Both the low level of genetic variation within the cultivated gene pool and its polyploid nature limit the utilization of molecular markers to explore genome structure and facilitate genetic improvement. Nevertheless, a wealth of genetic diversity exists in diploid Arachis species (2n = 2x = 20, which represent a valuable gene pool for cultivated peanut improvement. Interspecific populations have been used widely for genetic mapping in diploid species of Arachis. However, an intraspecific mapping strategy was essential to detect chromosomal rearrangements among species that could be obscured by mapping in interspecific populations. To develop intraspecific reference linkage maps and gain insights into karyotypic evolution within the genus, we comparatively mapped the A- and B-genome diploid species using intraspecific F2 populations. Exploring genome organization among diploid peanut species by comparative mapping will enhance our understanding of the cultivated tetraploid peanut genome. Moreover, new sources of molecular markers that are highly transferable between species and developed from expressed genes will be required to construct saturated genetic maps for peanut. Results A total of 2,138 EST-SSR (expressed sequence tag-simple sequence repeat markers were developed by mining a tetraploid peanut EST assembly including 101,132 unigenes (37,916 contigs and 63,216 singletons derived from 70,771 long-read (Sanger and 270,957 short-read (454 sequences. A set of 97 SSR markers were also developed by mining 9,517 genomic survey sequences of Arachis. An SSR-based intraspecific linkage map was constructed using an F2 population derived from a cross between K 9484 (PI 298639 and GKBSPSc 30081 (PI 468327 in the B-genome species A. batizocoi. A high degree of macrosynteny was observed

  5. Bradyrhizobium subterraneum sp. nov., a symbiotic nitrogen-fixing bacterium from root nodules of groundnuts.

    Science.gov (United States)

    Grönemeyer, Jann Lasse; Chimwamurombe, Percy; Reinhold-Hurek, Barbara

    2015-10-01

    Seven strains of symbiotic bacteria from root nodules of local races of Bambara groundnut (Vigna subterranea) and peanuts (Arachis hypogaea) grown on subsistence farmers' fields in the Kavango region, Namibia, were previously characterized and identified as a novel group within the genus Bradyrhizobium. To corroborate their taxonomic status, these strains were further characterized using a polyphasic approach. All strains possessed identical 16S rRNA gene sequences with Bradyrhizobium yuanmingense CCBAU 10071T being the most closely related type strain in the 16S rRNA gene phylogenetic analysis, and Bradyrhizobium daqingense CCBAU 15774T in the ITS sequence analysis. Phylogenetic analysis of concatenated glnII-recA-rpoB-dnaK placed the strains in a highly supported lineage distinct from named species of the genus Bradyrhizobium, most closely related to Bradyrhizobium yuanmingense CCBAU 10071T. The species status was validated by results of DNA–DNA hybridization. Phylogenetic analysis of nifH genes placed the novel strains in a group with nifH of ‘Bradyrhizobium arachidis’ CCBAU 051107 that also nodulates peanuts. The combination of phenotypic characteristics from several tests including carbon source utilization and antibiotic resistance could be used to differentiate representative strains from recognized species of the genus Bradyrhizobium. Novel strain 58 2-1T induced effective nodules on V. subterranea, Vigna unguiculata and A. hypogaea, and some strains on Lablab purpureus. Based on the data presented, we conclude that our strains represent a novel species for which the name Bradyrhizobium subterraneum sp. nov. is proposed, with 58 2-1T [ = DSM 100298T = LMG 28792T = NTCCM0016T (Windhoek)] as the type strain. The DNA G+C content of strain 58 2-1T was 64.7 mol% (T m). PMID:26198108

  6. Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa.

    Science.gov (United States)

    Huang, B L; Zhang, X K; Li, Y Y; Li, D Y; Ma, M Y; Cai, D T; Wu, W H; Huang, B Q

    2016-01-01

    Eruca vesicaria subsp sativa is one of the most tolerant Cruciferae species to drought, and dehydration-responsive element-binding protein 2A (DREB2A) is involved in responses to salinity, heat, and particularly drought. In this study, a gene encoding EvDREB2A was cloned and characterized in E. vesicaria subsp sativa. The full-length EvDREB2A cDNA sequence contained a 388-bp 5'-untranslated region (UTR), a 348-bp 3'-UTR, and a 1002-bp open reading frame that encoded 334 amino acid residues. The theoretical isoelectric point of the EvDREB2A protein was 4.80 and the molecular weight was 37.64 kDa. The genomic sequence of EvDREB2A contained no introns. Analysis using SMART indicated that EvDREB2A contains a conserved AP2 domain, similar to other plant DREBs. Phylogenetic analysis revealed that EvDREB2A and DREB2As from Brassica rapa, Eutrema salsugineum, Arabidopsis thaliana, Arabidopsis lyrata, and Arachis hypogaea formed a small subgroup, which clustered with DREB2Bs from A. lyrata, A. thaliana, Camelina sativa, and B. rapa to form a larger subgroup. EvDREB2A is most closely related to B. rapa DREB2A, followed by DREB2As from E. salsugineum, A. thaliana, A. hypogaea, and A. lyrata. A quantitative real-time polymerase chain reaction indicated that EvDREB2A expression was highest in the leaves, followed by the roots and hypocotyls, and was lowest in the flower buds. EvDREB2A could be used to improve drought tolerance in crops. PMID:27525923

  7. Qualidades física e fisiológica de sementes de amendoim submetidas a gesso agrícola: I. Área com calagem Physical and physiological qualities of peanut seed from plants cultivated with phosphogypsum source: I. Limed area

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    Maria Cristina Mingues Spinola

    2000-03-01

    Full Text Available O presente trabalho foi conduzido com sementes de amendoim (Arachis hypogaea L., cv. "Tatu", em blocos casualizados, com três repetições. Os tratamentos consistiram de doses de gesso agrícola (0, 400, 800, 1200 kg/ha, épocas de aplicação (semeadura e florescimento e localização do gesso agrícola (área total e sulcos de semeadura em área previamente submetida à calagem (70% de saturação de bases. Foi realizada adubação de semeadura à base de fósforo (80kg/ha de P2O5 e potássio (30kg/ha de K2O5. As avaliações da qualidade das sementes foram realizadas por meio da massa de 100 sementes, teste de germinação, testes de vigor (primeira contagem de germinação, envelhecimento acelerado, condutividade elétrica, emergência de plântulas em campo e determinação dos macronutrientes contidos nas sementes. O gesso agrícola em área calcareada, independentemente das doses, épocas e locais de aplicação, não interferiu na qualidade das sementes. A aplicação de gesso agrícola na semeadura, proporcionou um teor de nitrogênio nas sementes superior ao encontrado quando aplicado no florescimento. O enxofre elementar presente no gesso agrícola influenciou o teor de potássio nas sementes.This work was carried out with peanut seeds, cv.’Tatu‘(Arachis hypogaea L., in a randomized block design with three replications. The treatments were of phosphogypsum rates (0, 400, 800, 1200 kg/ha, application times at (sowing or flowering and application methods (total area or in bands in a limed area (base saturation up to 70%. Fertilization at sowing consisted of Phosphorus (80 kg/ha of P2O5 and Potassium (30 kg/ha of K2O5. Seed quality was evaluatted by weigth of 100 seeds, germination test, vigor tests (first germination counting, accelerated aging, eletric conductivity, and field seedling emergence and evaluation of macronutrient levels in seeds. Phosphogypsum in a limed area, independently of rate, application time and application

  8. Influência da calagem, da época de colheita e da secagem na incidência de fungos e aflatoxinas em grãos de amendoim armazenados Storage peanut kernels fungal contamination and aflatoxin as affected by liming, harvest time and drying

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    Claudia Antonia Vieira Rossetto

    2005-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a contaminação e o potencial para síntese de aflatoxinas pelos isolados do grupo Aspergillus flavus em grãos armazenados de amendoim (Arachis hypogaea L., que foram produzidos com distintos procedimentos de calagem, de colheita e de secagem. Para isto, foram avaliadas doze amostras de grãos de amendoim, cv. Botutatu, provenientes de plantas cultivadas em área que recebeu ou não a aplicação de calcário, colhidas aos 104, 114 e 124 dias após a semeadura e secas em condições ambientais e em estufa. Aos 12 e 18 meses de armazenamento, os grãos foram tratados com hipoclorito de sódio e incubados em BDA, a 20°C, por cinco dias. As espécies do grupo Aspergillus flavus foram identificadas após incubação em meio ADM. Posteriormente, o potencial toxígeno foi avaliado pelo método da cromatografia de camada delgada. A análise da freqüência de fungos revelou que os grãos de amendoim armazenados estavam contaminados por Aspergillus spp., Penicillium spp. e Fusarium spp. Os grãos de amendoim, provenientes da colheita antecipada, apresentaram maior contaminação pelo grupo Aspergillus flavus, sendo menor a proporção destes com potencial toxígeno.The objective of this work was to evaluate the effect of the storage on the potential of aflatoxin production by isolates from Aspergillus flavus group in peanut (Arachis hypogaea L.. These kernels were obtained from a field experiment with two areas (with or without lime, three times of harvest (104, 114 and 124 days after planting and two types of dryer conditions (ambient and chamber with forced air. After 12 and 18 months of storage, the kernels were treated with sodium hypochloride and incubated in a PDA at 20°C during five days. The isolates from Aspergillus flavus group were identified after incubation in ADM culture medium. The toxigenic potential was analyzed by thin layer chromatography. The genera detected were Aspergillus, Penicillium and

  9. Avaliação da micoflora e ocorrência de micotoxinas em cascas de amendoim em diferentes estágios de maturação da vagem Evaluation of mycoflora and occurrence of mycotoxins in peanut hulls at different pod maturation stages

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    Edlayne Gonçalez

    2008-10-01

    Full Text Available As cascas de amendoim (Arachis hypogaea L. são de grande importância para confecção de cama de frangos, de gado de leite e como fonte de fibras para ruminantes, portanto a elucidação dos mecanismos de contaminação por fungos toxigênicos e por micotoxinas em amendoim é imprescindível, especialmente para que medidas preventivas possam ser tomadas. Realizou-se, este trabalho, em Junqueirópolis, Estado de São Paulo, Brasil. Os principais fungos isolados nas cascas de amendoim foram Fusarium ssp. (78,75 %, Rhizopus ssp. (14,1 % e A. flavus (11,75 %. No solo foram isolados Penicillium spp., Fusarium spp. e Aspergillus flavus, entre outros. Aflatoxinas foram detectadas em amostras de cascas de amendoim a partir do estágio de granação em concentrações que variaram de 5,42 μg/kg a 218,52 μg/kg. Ácido ciclopiazônico e fumonisinas B1 e B2 não foram detectadas. A presença de A. flavus e aflatoxinas nas amostras, revela a importância de um controle das cascas de amendoim antes de sua utilização. Boas práticas agrícolas são indicadas para região, uma vez que a contaminação das vagens ocorreu antes da colheita.Peanut (Arachis hypogaea L. hulls are very important because they are used as litter to poultry and dairy cattle and as fiber source to cattle. The elucidation of the peanuts contamination mechanisms by toxigenic fungi and their mycotoxins is vital, specially for prevention measurements. The peanuts total mycoflora and mycotoxin contamination were analyzed in plants sampled in Junqueirópolis, in São Paulo State (Brazil at different stages of the pod maturity. The prevalent mycoflora in peanut hulls were Fusarium spp., Rhizopus spp. and Aspergillus flavus. In soil under the peanut crop, the genus Penicillium spp., Fusarium spp. and A. flavus were detected. Aflatoxins were detected in peanut hull samples since filling pod stage in concentrations from 5.42 μg/kg to 218.52 μg/kg. Cyclopiazonic acid and fumonisins were not

  10. Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data

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    Zhou Sun

    2012-05-01

    Full Text Available Abstract Background Expressed Sequence Tag (EST sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be “unclean”. Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data quality control and accurate delineation of transcription ends, but also furthers our understanding of the potential sources of data abnormalities/errors present in the wet-lab procedures for cDNA library construction. Results After analyzing a total of 309,976 raw Pinus taeda ESTs, we uncovered many distinct variations of cDNA termini, some of which prove to be good indicators of wet-lab artifacts, and characterized each raw EST by its cDNA terminus structure patterns. In contrast to the expected patterns, many ESTs displayed complex and/or abnormal patterns that represent potential wet-lab errors such as: a failure of one or both of the restriction enzymes to cut the plasmid vector; a failure of the restriction enzymes to cut the vector at the correct positions; the insertion of two cDNA inserts into a single vector; the insertion of multiple and/or concatenated adapters/linkers; the presence of 3′-end terminal structures in designated 5′-end sequences or vice versa; and so on. With a close examination of these artifacts, many problematic ESTs that have been deposited into public databases by conventional bioinformatics pipelines or tools could be cleaned or filtered by our methodology. We developed a software tool for Abnormality Filtering and Sequence Trimming for ESTs (AFST, http://code.google.com/p/afst/ using a pattern analysis approach. To compare AFST with other pipelines that submitted ESTs into dbEST, we reprocessed 230,783 Pinus taeda and 38,709 Arachis hypogaea GenBank ESTs. We found 7.4% of Pinus taeda and 29.2% of Arachis hypogaea GenBank ESTs are “unclean” or abnormal, all of which could be cleaned

  11. Análise histoquímica foliar do amendoim: genótipos 'Tatu' e SO-909 Leaf histochemical analyses of peanut: genotypes 'Tatu' and SO-909

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    Renato Ferraz de Arruda Veiga

    1992-01-01

    Full Text Available Este trabalho teve por finalidade a análise histoquímica foliar de dois genótipos de amendoim (Arachis hypogaea L., do tipo botânico Valência: SO-53 ('Tatu' e SO-909 (PI-259747, cuja literatura demonstra apresentarem respostas diferentes de resistência às principais moléstias fúngicas foliares do Brasil. Seções transversais das seguintes estruturas - pulvino, haste peciolar, raque, pulvínulo e folíolo - e seções paradérmicas de folíolos coletados em dois anos agrícolas consecutivos, foram analisadas quanto à presença de alcalóides, amido, calose, celulose pura, celulose com pectina, cera, cristais, cutina, lignina, mucilagem, óleo, resina, tanino e ureídeos (micrograma por folíolo (grama. As diferenças qualitativas histoquímicas observadas nos diversos tecidos, como a freqüência de tanino, alcalóide, pectina e óleo, supostamente, podem ser responsáveis pela resistência ou suscetibilidade dos genótipos às moléstias fúngicas foliares. Para fins de caracterização, mostrou-se eficiente a avaliação de pureza de celulose.Leaf histochemical analyses were made in two genotypes of Arachis hypogaea L., of the Valencia group, which present different responses to some of the peanut foliar diseases. The analyses were performed on cross sections of the pulvini, petiole, rachis, pulvinulus and leaflets. The following constituents were observed: alkaloids, callose, cellulose with pectin, cristal, cutin, lignin, mucilage, oil, pure cellulose, resin, starch, tannin, wax and weight of ureides by leaflets. Some histochemical characteristics such the amount of tannin, alkaloids, pectin and oil can be produce different responses of peanut to foliar fungal diseases, and can be used in the characterization of peanut genotypes like the amount of pure cellulose and cellulose with pectin.

  12. Efeito do espaçamento entre fileiras de amendoim rasteiro na interferência de plantas daninhas na cultura Effect of peanut crop row spacing on weed interference in the culture

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    T.C.S. Dias

    2009-06-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito da redução do espaçamento entre fileiras nos períodos de interferência e na produtividade do amendoim rasteiro (Arachis hypogaea. O experimento foi instalado no município de Jaboticabal-SP. Os tratamentos constaram de dois espaçamentos entre fileiras (80 e 90 cm, divididos em dois grupos. No primeiro, as plantas daninhas foram controladas desde a emergência até 0 (interferência constante, 30, 45, 60, 82, 97 e 112 dias depois. No segundo, as plantas daninhas conviveram com a cultura pelos mesmos períodos do grupo anterior. O delineamento experimental adotado foi o de blocos casualizados, em arranjo de parcelas subdivididas, com quatro repetições. As principais plantas daninhas presentes na área foram Digitaria sp., Xanthium strumarium, Acanthospermum hispidum e Cenchrus echinatus. Para uma perda tolerável de 5% de produtividade, o período crítico de prevenção à interferência foi dos 27 aos 76 e dos 35 aos 96 dias após a emergência para os espaçamentos de 80 e 90 cm, respectivamente; a queda de produtividade das parcelas mantidas com interferência de plantas daninhas em relação àquelas no limpo foi superior a 80%, independentemente do espaçamento.The aim of this research was to evaluate the effect of reducing the spacing between rows in periods of weed interference and peanut (Arachis hypogaea yield. The experiment was carried out in Jaboticabal, Brazil. Treatments consisted of two row spacings (80 to 90 cm, divided into two groups. In the first group, the weeds were controlled since emergence up to 0 (constant interference, 30, 45, 60, 82, 97 and 112 days. In the second group, the weeds were allowed to grow with the peanut culture during the same periods. The experiment was arranged in a randomized block design with treatments in split-plots, with four repetitions. The main weeds in the area were Digitaria sp., Xanthium strumarium, Acanthospermum hispidum and Cenchrus

  13. Effect of replacing dietary soybean meal with tropical legumes on the performance of lambs

    International Nuclear Information System (INIS)

    This study determined how supplementing bahiagrass hay (Paspalum notatum Fluegge cv. 'Pensacola') with soybean (Glycine max) meal or warm-season legume hays affected intake, digestibility, and N utilisation by lambs. Dorper x Katadhin crossbred lambs (30.6 ± 5.5 kg; n = 42) were fed bahiagrass hay ad libitum and supplemented with nothing (control), soybean meal, or hays of annual peanut (Arachis hypogaea), cowpea (Vigna unguiculata), perennial peanut (Arachis glabrata), pigeonpea (Cajanus cajan), or soybean. Legume hays were supplemented at 50% of diet dry matter (DM); soybean meal was supplemented at 4.25% of diet DM to match the average crude protein (CP) content (10.8%) of the legume hay supplemented diets. Cowpea, pigeonpea, and soybean were harvested at maturities that maximised DM yield and nutritive value, and peanuts were first cuttings. Diets were fed to six lambs per treatment for two consecutive 21-d periods. Supplementation with annual and perennial peanut, cowpea, and soybean hay increased (P < 0.01) DM intake versus control, but apparent DM digestibility was only increased (P = 0.03) by supplementation with either peanut. Nitrogen intake, digestibility, and retention were increased (P < 0.01) by supplementation particularly with annual or perennial peanut hay. Ruminal ammonia concentration was increased (P < 0.01) by all legume hay supplements versus the control. Microbial N synthesis and ruminally degraded organic matter (OM) were increased (P = 0.03) by perennial and annual peanut hay supplementation, but the efficiency of microbial synthesis was not different (P = 0.52) among diets. Annual and perennial peanut hays were the best supplements for the bahiagrass hay in this study. (author)

  14. Response of Groundnut (�JL-24� Cultivar to Mycorrhiza Inoculation and Phosphorous Application

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    Khirood DOLEY

    2012-08-01

    Full Text Available A pot experiment was conducted on peanut Arachis hypogaea L. during 2007 growing season to determine their growth characteristics due to mycorrhizal inoculation and two different levels of soluble phosphorous application. Due to inoculation by AM fungi the growth parameters such as leaf number, shoot length, root length, fresh weight, dry weight, pod number and nodule number were significantly increased but two different level of phosphate also showed growth. However, growth parameters showed variable results when two different level of phosphate was applied along with AM fungi. Without phosphorous the mycorrhizal groundnut showed significant growth but when first low level of phosphorous was applied it showed more significant growth, however most significant result was observed with second high level of phosphorous application to the groundnut plant. Total chlorophyll content and acid and alkaline phosphatase activity was also significantly higher but most significant were observed when first level of phosphorous was applied followed by second level of phosphorous. The percent root colonization by mycorrhizal fungus Glomus fasciculatum was higher due to application of phosphorous but mycorrhizal dependency went on decreasing due to increase in the level of phosphorous. The different level of phosphorous had significant effect on growth and physiological parameters of mycorrhizal and non-mycorrhizal Arachis plants after 30, 60 and 90 days of growth period. However, the obtained results proved the improvement in plant growth with application of phosphorous. Thus, for increase in production of groundnut in the state of Maharashtra seems to be feasible option for increasing the overall production and yield.

  15. Adaptation à la sécheresse et création variétale : le cas de l’arachide en zone sahélienne

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    Clavel Danièle

    2005-05-01

    Full Text Available Drought is a multiform constraint expressing at different plant organisation levels. It is recognised as the first factor limiting the agriculture production in the world. The Sahel subtropical regions were the most exposed to the devasting effects of the recent climate change. A state of knowledge referring to the plant responses to drought is a fundamental initial step to any program of selection. This review concerns more particularly groundnut (Arachis hypogaea L., which is a very common legume in the Sahel areas. Critical evaluations of traits as relevant selection criteria for a breeding program aiming at improving yield and yield stability under drought constraint were made. The genetic specificities of the species are exposed in the first part. Then a detailed description of the grain-legume traits related to drought adaptation is presented. The review was widened to other plants for the molecular responses to water deficit, supposed to be more general. Finally, the last part supplied an updated synthesis of the recent advances in biotechnology on groundnut with special focus on drought tolerance.

  16. Glutathione S-transferase SlGSTE1 in Spodoptera litura may be associated with feeding adaptation of host plants.

    Science.gov (United States)

    Zou, Xiaopeng; Xu, Zhibin; Zou, Haiwang; Liu, Jisheng; Chen, Shuna; Feng, Qili; Zheng, Sichun

    2016-03-01

    Spodoptera litura is polyphagous pest insect and feeds on plants of more than 90 families. In this study the role of glutathione S-transferase epilson 1 (slgste1) in S. litura in detoxification was examined. This gene was up-regulated in the midgut of S. litura at the transcriptional and protein levels when the insect fed on Brassica juncea or diet containing phytochemicals such as indole-3-carbinol and allyl-isothiocyanate that are metabolic products of sinigrin and glucobrassicin in B. juncea. The SlGSTE1 could catalyze the conjugation of reduced glutathione and indole-3-carbinol and allyl-isothiocyanate, as well as xanthotoxin, which is a furanocoumarin, under in vitro condition. When the expression of Slgste1 in the larvae was suppressed with RNAi, the larval growth and feeding rate were decreased. Furthermore, the up-regulated expression of the SlGSTE1 protein in the midgut of larvae that fed on different host plants was detected by 2-DE and ESI/MS analysis. The feeding adaptation from the most to the least of the larvae for the various host plants was Brassica alboglabra, Brassica linn. Pekinensis, Cucumis sativus, Ipomoea batatas, Arachis hypogaea and Capsicum frutescens. All the results together suggest that Slgste1 is a critical detoxifying enzyme that is induced by phytochmicals in the host plants and, inter alia, may be related to host plant adaptation of S. litura. PMID:26631599

  17. Case Study: Trap Crop with Pheromone Traps for Suppressing Euschistus servus (Heteroptera: Pentatomidae in Cotton

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    P. G. Tillman

    2012-01-01

    Full Text Available The brown stink bug, Euschistus servus (Say, can disperse from source habitats, including corn, Zea mays L., and peanut, Arachis hypogaea L., into cotton, Gossypium hirsutum L. Therefore, a 2-year on-farm experiment was conducted to determine the effectiveness of a sorghum (Sorghum bicolor (L. Moench spp. bicolor trap crop, with or without Euschistus spp. pheromone traps, to suppress dispersal of this pest to cotton. In 2004, density of E. servus was lower in cotton fields with sorghum trap crops (with or without pheromone traps compared to control cotton fields. Similarly, in 2006, density of E. servus was lower in cotton fields with sorghum trap crops and pheromone traps compared to control cotton fields. Thus, the combination of the sorghum trap crop and pheromone traps effectively suppressed dispersal of E. servus into cotton. Inclusion of pheromone traps with trap crops potentially offers additional benefits, including: (1 reducing the density of E. servus adults in a trap crop, especially females, to possibly decrease the local population over time and reduce the overwintering population, (2 reducing dispersal of E. servus adults from the trap crop into cotton, and (3 potentially attracting more dispersing E. servus adults into a trap crop during a period of time when preferred food is not prevalent in the landscape.

  18. Efficiency of green manure species on the population of reniform nematode

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    Cristiane Gonçalves Gardiano

    2014-02-01

    Full Text Available The objective of this study was to evaluate the growing of soil improving crops on the population of Rotylenchulus reniformis in naturally infested soil. It was evaluated the effect of 6 species of plants as cover crops in winter and 13 summer species and a fallow treatment on the nematode population under greenhouse. After 60 days, the root system was collected. Then, a sample of soil was taken in order to extract juveniles from the soil and quantification the final population of the pathogen in each pot for determining of the reproduction factor (RF. Fallow and all winter species of green manure, except hairy vetch, reduced the population of R. reniformis after cultivation in infested soil, in comparison to the control. Regarding summer cover crops, it was observed that sorghum ‘SI03204’ (Sorghum vulgare, millet ‘BRS1501’ (Pennisetum glaucum, Brachiaria ruziziensis, finger millet (Eleusine coracana, estylo ‘Campo Grande’ (Stylosanthes capitata x S. macrocephala, peanut ‘IAC Tatu ST’ (Arachis hypogaea and dwarf velvet bean (Mucuna deeringiana reduced the population of R. reniformis, when compared to the control, could be used in the management of this nematode.

  19. Effects of drought on cadmium accumulation in peanuts grown in a contaminated calcareous soil.

    Science.gov (United States)

    Xia, Shenglan; Wang, Xvming; Su, Genqiang; Shi, Gangrong

    2015-12-01

    This study aimed to investigate the effects of drought stress on cadmium (Cd) accumulation in peanut (Arachis hypogaea L.) grown in contaminated calcareous soils. Five peanut cultivars were grown in a calcareous soil spiked with 4 mg Cd kg(-1) soil (dry weight) under well-watered, mild drought, and severe drought conditions. The biomass production, gas exchange, spectral reflectance, and Cd accumulation in plant tissues were determined. The five cultivars significantly differed from each other in biomass production, gas exchange, spectral reflectance, and Cd accumulation. The effect of drought on Cd accumulation in peanuts varies with plant tissues, cultivars, and developmental stages. Drought decreased root Cd concentrations in seedlings of the two high Cd-accumulating cultivars (Haihua 1 and Zhenghong 3), which is associated with increasing leaf active Fe content. However, for the mature plants, drought stress caused an increase in Cd accumulation in roots, pod walls, and seeds depending on peanut cultivars. Negative correlations were found between seed Cd concentration and biomasses in both preflowering seedlings and mature plants. The seed Cd concentration in mature plants was also observed to be positively correlated with the shoot Cd concentration in preflowering seedlings. The increased Cd concentration in seeds of drought-stressed peanut plants grown in Cd-contaminated calcareous soils might be attributed to the drought-induced decrease of biomass production. PMID:26194243

  20. Conjoint effect of oil-seed cakes and Pseudomonas fluorescens on the growth of chickpea in relation to the management of plant-parasitic nematodes

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    Rose Rizvi

    2012-12-01

    Full Text Available Soil application of organics has been explored as an alternative means of organic management of plant-parasitic nematodes. Efficiency of different oil-seed cakes of neem (Azadirachta indica, castor (Ricinus communis, groundnut (Arachis hypogaea, linseed (Linum usitatissimum, sunflower (Helianthus annuus and soybean (Glycine max were evaluated in field conditions with association of Pseudomonas fluorescens in relation to growth parameters of chickpea and population of plant-parasitic nematodes. Their efficacious nature was highly effective in reducing the population of these dominant soil nematodes. Significant improvement was observed in plant-growth parameters such as plant weight, percent pollen fertility, pod numbers, root-nodulation and chlorophyll content of chickpea, seemed to be due to reduction in disease incidence and might be due to growth promoting substances secreted by P. fluorescens. The multiplication rate of nematodes was less in the presence of P. fluorescens as compared to its absence. Most effective combination of P. fluorescens was observed with neem cake.

  1. Selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L. on the sand sterile medium in greenhouse

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    SRI PURWANINGSIH

    2005-07-01

    Full Text Available An experiment on the selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L. on the sand sterile medium in green house. The aim of the experiment the selection and potency of the Rhizobium strain to increase the growth of soybean. The experiment was carried out in green house condition in Microbiology Division, Research Center for Biology-LIPI with sterile sand medium. The research design was Completely Randomized Design with three replications for each treatment. The Rhizobium strains used were 1 W (isolated from bean, Vigna radiata, 2 W (isolated from soybean, 3 W (isolated from bean, 4 W (isolated from soybean, 5 W (isolated from soybean, 6 W (isolated from peanut, Arachis hypogaea, 7 W (isolated from peanut, 8 W (isolated from peanut, the controls were uninoculated with Rhizobium strain and without urea fertilizer (K1, uninoculated and with urea fertilizer equal 100 kg/ha (K2. The plants were harvested after 50 days, the variable of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules and ‘symbiotic capacity”. The results showed that all of experiment plant which be inoculated with Rhizobium able to form nodule. Strain of 2 W (isolated from soybean has given the best effects on the growth of soybean.

  2. Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol

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    Tengale Dipak Bhauso

    2014-01-01

    Full Text Available In the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L. crop yield. In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. Mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many plant species. However, peanut plant is not known to synthesize mannitol. Therefore, bacterial mtlD gene coding for mannitol 1-phosphate dehydrogenase under the control of constitutive promoter CaMV35S was introduced and overexpressed in the peanut cv. GG 20 using Agrobacterium tumefaciens-mediated transformation. A total of eight independent transgenic events were confirmed at molecular level by PCR, Southern blotting, and RT-PCR. Transgenic lines had increased amount of mannitol and exhibited enhanced tolerance in response to water-deficit stress. Improved performance of the mtlD transgenics was indicated by excised-leaf water loss assay and relative water content under water-deficit stress. Better performance of transgenics was due to the ability of the plants to synthesize mannitol. However, regulation of mtlD gene expression in transgenic plants remains to be elucidated.

  3. Weed Control and Peanut Tolerance with Ethalfluralin-Based Herbicide Systems

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    W. J. Grichar

    2012-01-01

    Full Text Available Field studies were conducted from 2007 through 2009 to determine weed efficacy and peanut (Arachis hypogaea L. response to herbicide systems that included ethalfluralin applied preplant incorporated. Control of devil's claw (Proboscidea louisianica (Mill. Thellung, yellow nutsedge (Cyperus esculentus L., Palmer amaranth (Amaranthus palmeri S. Wats., and puncturevine (Tribulus terrestris L. was most consistent with ethalfluralin followed by either imazapic or imazethapyr applied postemergence. Peanut stunting was 19% when paraquat alone was applied early-postemergence. Stunting increased to greater than 30% when ethalfluralin applied preplant incorporated was followed by S-metolachlor applied preemergence and paraquat applied early-postemergence. Stunting (7% was also observed when ethalfluralin was followed by flumioxazin plus S-metolachlor applied preemergence with lactofen applied mid-postemergence. Ethalfluralin followed by paraquat applied early-postemergence reduced peanut yield when compared to the nontreated check. Ethalfluralin applied preplant incorporated followed by imazapic applied mid-postemergence provided the greatest yield (6220 kg/ha. None of the herbicide treatments reduced peanut grade (sound mature kernels plus sound splits when compared with the nontreated check.

  4. Early events of secretory granule formation in the rat parotid acinar cell under the influence of isoproterenol. An ultrastructural and lectin cytochemical study

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    F D’Amico

    2009-12-01

    Full Text Available The events involved in the maturation process of acinar secretory granules of rat parotid gland were investigated ultrastructurally and cytochemically by using a battery of four lectins [Triticum vulgaris agglutinin (WGA, Ulex europaeus agglutinin I (UEA-I, Glycine max agglutinin (SBA, Arachys hypogaea agglutinin (PNA]. In order to facilitate the study, parotid glands were chronically stimulated with isoproterenol to induce secretion. Specimens were embedded in the Lowicryl K4M resin. The trans-Golgi network (TGN derived secretory granules, which we refer to as immature secretory granules, were found to be intermediate structures in the biogenesis process of the secretory granules in the rat parotid acinar cell. These early structures do not seem to be the immediate precursor of the mature secretory granules: in fact, a subsequent interaction process between these early immature granule forms and TGN elements seems to occur, leading, finally, to the mature granules. These findings could explain the origin of the polymorphic subpopulations of the secretory granules in the normal acinar cells of the rat parotid gland. The lectin staining patterns were characteristic of each lectin. Immature and mature secretory gran- ules were labelled with WGA, SBA, PNA, and lightly with UEA-I. Cis and intermediate cisternae of the Golgi apparatus were labelled with WGA, and trans cisternae with WGA and SBA.

  5. Determination of the phytoalexin resveratrol (3,5,4'-trihydroxystilbene) in peanuts and pistachios by high-performance liquid chromatographic diode array (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS).

    Science.gov (United States)

    Tokuşoglu, Ozlem; Unal, Mustafa Kemal; Yemiş, Fadim

    2005-06-15

    The phytoalexin resveratrol (3,5,4'-trihydroxystilbene) in edible peanut (Arachis hypogaea L.) and pistachio (Pistacia vera L.) varieties grown in Turkey was analyzed by high-performance liquid chromatographic diode array and gas chromatography-mass spectrometric detection. trans-Resveratrol in six peanut varieties, five pistachio varieties, and four market samples ranged between 0.03 and 1.92 microg/g. The Cerezlik 5025 peanut (1.92 +/- 0.01 microg/g) and Ohadi pistachio genotype (1.67 +/- 0.01 microg/g) had significantly higher trans-resveratrol contents. Peanuts contained 0.03-1.92 microg/g (av = 0.84 microg/g) of trans-resveratrol, whereas pistachio contained 0.09-1.67 microg/g (av = 1.15 microg/g). With exposure to UV light for 1 min, trans-resveratrol concentrations of samples ranged from 0.02 to 1.47 microg/g and those of cis-resveratrol from 0.008 to 0.32 microg/g. The occurrence of resveratrol in peanut and pistachio was confirmed by total ion chromatograms (TIC) of bis[trimethylsilyl]trifluoroacetamide derivatives of resveratrol isomers and comparison of the mass spectral fragmentation data with those of a resveratrol standard. Formation of the cis-isomer in pistachios was higher than in peanuts. PMID:15941348

  6. Techniques for sperm evaluation using fluorescent probes

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    Andrielle Thainar Mendes Cunha

    2015-12-01

    Full Text Available  A variety of laboratory tests were developed to obtain more reliable results of sperm evaluation and increase the accuracy of sperm fertility predictions. These tests detected damage of sperm specific compartments or organelles, which cannot be detected in routine sperm analysis. The use of fluorescent probes and detection using fluorescent microscopy or flow cytometry is an important tool but a more precise and accurate laboratory test is needed. Propidium iodide and 6-carboxyfluorescein diacetate are used for evaluations of plasmatic membrane integrity. Fluorescein isothiocyanate, associated with conjugated lecithin Psium sativum or Arachis hypogaea, are used for evaluations of acrosome integrity. Two probes, MitoTracker or Rhodamine123, are generally used to measure the absence or presence of mitochondrial potential. However, a better option is 5,5’; 6,6’ - tetrachloro - 1,1’; 3,3’ -tetraetilbenzimidazolil-carbocyanine (JC-1 dye, which assesses not only the presence of mitochondrial potential and distinguished spermatozoa with poorly and highly functional mitochondria. Two techniques, TUNEL or COMETA, and the Acridine Orange Test (AOT dye are used to evaluate chromatin integrity. A fluorescence technique based on chlortetracycline (CTC or Merocyanine 540 is used to estimate whether sperm pass by or through the capacitation process. This review focuses on the fluorescent probes that are most widely used to evaluate plasma membrane integrity, capacitation, acrosome integrity, chromatin integrity and mitochondrial potential.

  7. Evaluation of BNF by groundnut and responses of cereal crops to different levels of nitrogen fertilizer in the coastal area of the Syrian Arab Republic

    International Nuclear Information System (INIS)

    A two course crop rotation experiment was conducted over a period of two years in order to evaluate the biological nitrogen fixation (BNF) by groundnut (Arachis hypogaea) and its contribution to the subsequent cereal crop in terms of its N-conserving effect. Also the response of the treatment crop (Zea mays L.) to different levels of N-fertilization (100 and 150 kg N ha-1) were evaluated. Moreover, the effect of a previous crop, N rate and timing on the test crop (Triticum aestivum) was assessed. Results showed that groundnut fixed as much as 52.9 and 23.4 kg N ha-1 at pod filling stage and 66.7 and 34.4 at physiological maturity stage for the 1992 and 1993 growing season, respectively. The test crop did not benefit from the residual N due to the high precipitation in the region leaching down most of the inorganic nitrogen beyond the root zone. In the 1992 growing season, the lower N rate for maize (100 kg N ha-1) was superior over the higher rate (150 kg N ha-1). But due to water stress in the 1993 growing season, a different trend with regard to the response of maize to fertilizer N was obtained. (author). 14 refs, 1 fig., 10 tabs

  8. Induced plant responses to pathogen attack. Analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (Glycine max L. Merr. cv. Harosoy 63).

    Science.gov (United States)

    Welle, R; Schröder, G; Schiltz, E; Grisebach, H; Schröder, J

    1991-03-14

    In soybean (Glycine max L.), pathogen attack induces the formation of glyceollin-type phytoalexins. The biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. Screening of a soybean cDNA library from elicitor-induced RNA in lambda gt11 yielded two classes of reductase-specific clones. The deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. Four clones of class A were expressed in Escherichia coli, and the proteins were tested for enzyme activity in extracts supplemented with chalcone synthase. All were active in 4,2',4'-trihydroxychalcone formation, and the quantification showed that shorter lengths of the cDNAs at the 5' end correlated with progressively decreasing enzyme activities. Genomic blots with DNA from plants capable of 4,2',4'-trihydroxychalcone synthesis revealed related sequences in bean (Phaseolus vulgaris L.) and peanut (Arachis hypogaea L.), but not in pea (Pisum sativum L.). No hybridization was observed with parsley (Petroselinum crispum) and carrot (Daucus carota) which synthesize other phytoalexins. The reductase protein contains a leucine-zipper motif and reveals a marked similarity with other oxidoreductases most of which are involved in carbohydrate metabolism. PMID:1840523

  9. Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut.

    Science.gov (United States)

    Wang, Pengfei; Li, Changsheng; Li, Cui; Zhao, Chuanzhi; Xia, Han; Zhao, Shuzhen; Hou, Lei; Gao, Chao; Wan, Shubo; Wang, Xingjun

    2015-07-01

    WUSCHEL-related homeobox (WOX) genes play key roles in plant stem cell maintenance and development. WOX genes showed specific expression patterns which are important for their functions. WOX13 subfamily genes as the ancestor genes of this family were less studied in the past. In this study, we cloned three Arachis hypogaea (peanut) WOX13 (AhWOX13) subfamily genes from peanut: WOX13A and WOX13B1, 2. WOX13B1 encoded a same protein as WOX13B2, and there were only two-base difference between these two genes. Differential expression patterns were observed for these three AhWOX13 subfamily genes in different tissues and developmental stages. Phylogenic trees analysis showed that these AhWOX13 subfamily genes were the most conserved WOX genes and belonged to the ancient clade of WOX family. This was also supported by the conserved motif analysis. Selective pressure analysis showed that the WOX family genes mainly underwent weak purifying selection (ω = 0.58097), while many positive mutations accumulated during the evolution history. Under the purifying selection, gene duplication event and loss of duplicated gene play important roles in the expansion and evolution of WOX family. PMID:26115849

  10. Lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. Lectin binding patterns of schwannoma and neurofibroma.

    Science.gov (United States)

    Matsumura, K; Nakasu, S; Nioka, H; Handa, J

    1993-01-01

    Lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. Tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. Changes of glycoconjugates were observed in association with a neoplastic transformation of Schwann cells; Arachis hypogaea (PNA) staining after neuraminidase treatment seen in normal Schwann cells was reduced in schwannoma of Antoni type A, and bindings with Glycine max (SBA) and Helix pomatia (HPA) after sialic acid removal, which were not seen in normal Schwann cells, appeared in schwannoma cells. Intensities of staining of tumor cells with each lectin were higher in Antoni type B than those in Antoni type A. No differences in lectin binding patterns were observed between schwannomas in patients with neurofibromatosis or recurrent schwannomas and ordinary, primary schwannomas in patients without stigmata of neurofibromatosis. Lectin binding patterns of Schwann cells and perineurial cells in neurofibroma were almost similar to those in normal peripheral nerves with an exception of faint stain of Schwann cells with HPA after neuraminidase pretreatment. This result suggests differences in extent of differentiation between schwannoma cells and neoplastic Schwann cells in neurofibroma. Specific PNA binding to perineurial cells in neurofibroma indicates the significance of this lectin as a marker of these cells. PMID:8310811

  11. Lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. Lectin binding pattern of normal peripheral nerve in man.

    Science.gov (United States)

    Matsumura, K; Nakasu, S; Nioka, H; Handa, J

    1993-01-01

    The binding patterns of lectins to normal peripheral nerves were examined. Twelve biotinylated lectins were used in this study; Canavalia ensiformis (Con A), Pisum sativum (PSA), Lens culinaris (LCA), Ricinus communis 1 (RCA-1), Arachis hypogaea (PNA), Glycine max (SBA), Sophora japonica (SJA), Bandeiraea simplicifolia 1 (BSL-1), Triticum vulgaris (WGA), succinylated WGA (s-WGA), Ulex europaeus 1 (UEA-1) and Helix pomatia (HPA). Cytoplasm of Schwann cells and perineurial cells was stained by Con A, PSA, LCA, s-WGA and WGA. PNA showed specific binding to perineurial cells, while after neuraminidase treatment stain with this lectin was demonstrated also in Schwann cells. Myelin sheaths were stained with fewer lectins. SBA and HPA with sialic acid removal rarely showed reactivity to the peripheral nerve structure in surgical specimens, in contrast to clear staining of Schwann cells, perineurial cells and myelin sheaths in autopsy specimens. The present study shows distinct lectin stainings of specific structures of the normal human peripheral nerves, and provides important basic information on the alterations of lectin binding patterns during pathological processes in the peripheral nerves. PMID:8310810

  12. Seed priming with extracts of Acacia nilotica (L.) Willd. ex Delile and Sapindus mukorossi (L.) plant parts in the control of root rot fungi and growth of plants

    International Nuclear Information System (INIS)

    Seed priming with plant extracts and chemicals has been used as an important growth enhancement tool in crop plants. In this research, an attempt was made to understand the mechanism of various seed priming treatments on greenhouse-grown okra (Abelmoschus esculentus (L.) Moench.), sunflower (Helianthus annuus L.), peanut (Arachis hypogaea L.) and chickpea (Cicer arietinum L.) for the control of root infecting fungi like Rhizoctonia solani (Kn), Fusarium spp. and Macrophomina phaseolina (Tassi) Goid by plant parts extracts (stem, leaves and seeds) of Acacia nilotica (L.) Willd. ex Delile and Sapindus mukorossi (L) at different time intervals (5, 10, 20, 40 minutes). Results showed significant suppression of root rot fungi and significantly enhanced the growth parameters like shoot length, root length, shoot weight and root weight. Seed-priming with A. nilotica and S. mukorossi leaves extract for 10 minutes time interval was found to be effective for the control of root rot fungi and growth of all tested leguminous and non-leguminous plants. (author)

  13. Crop candidates for the bioregenerative life support systems in China

    Science.gov (United States)

    Chunxiao, Xu; Hong, Liu

    The use of plants for life support applications in space is appealing because of the multiple life support functions by the plants. Research on crops that were grown in the life support system to provide food and oxygen, remove carbon dioxide was begun from 1960. To select possible crops for research on the bioregenerative life support systems in China, criteria for the selection of potential crops were made, and selection of crops was carried out based on these criteria. The results showed that 14 crops including 4 food crops (wheat, rice, soybean and peanut) and 7 vegetables (Chinese cabbage, lettuce, radish, carrot, tomato, squash and pepper) won higher scores. Wheat ( Triticum aestivum L.), rice ( Oryza sativa L.), soybean ( Glycine max L.) and peanut ( Arachis hypogaea L.) are main food crops in China. Chinese cabbage ( Brassica campestris L. ssp. chinensis var. communis), lettuce ( Lactuca sativa L. var. longifolia Lam.), radish ( Raphanus sativus L.), carrot ( Daucus carota L. var. sativa DC.), tomato ( Lycopersicon escalentum L.), squash ( Cucurbita moschata Duch.) and pepper ( Capsicum frutescens L. var. longum Bailey) are 7 vegetables preferred by Chinese. Furthermore, coriander ( Coriandum sativum L.), welsh onion ( Allium fistulosum L. var. giganteum Makino) and garlic ( Allium sativum L.) were selected as condiments to improve the taste of space crew. To each crop species, several cultivars were selected for further research according to their agronomic characteristics.

  14. Molecular cloning and expression of EgTCTP, encoding a calcium binding protein, enhances the growth of callus in oil palm (Elaeis guineensis, Jacq

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    Alisa Nakkaew

    2010-12-01

    Full Text Available The translationally controlled tumor protein (TCTP has now been identified in evolutionarily diverse organisms and isthought to play an important role in cell growth and cell division. We have identified an EgTCTP gene from Elaeis guineensisJacq. It is a putative protein of 168 amino acids with a calculated molecular mass of 19.2 kDa. EgTCTP has a high homology(84% - 91% identity at the amino acid level to other plant TCTPs from Hevea brasiliensis, Arachis hypogaea and Glycinemax. The recombinant EgTCTP protein is a calcium binding protein. Transgenic embryonic calli overexpressing EgTCTP havea faster growth rate than non-transformed and empty vector transformed calli. The results show that the enhancement ofEgTCTP gene expression in oil palm embryogenic calli may result in faster multiplication of the embryogenic calli. EgTCTPacts as another Ca2+-modulated protein that is involved in the cell cycle progression.

  15. The peanut allergy epidemic: allergen molecular characterisation and prospects for specific therapy.

    Science.gov (United States)

    de Leon, Maria P; Rolland, Jennifer M; O'Hehir, Robyn E

    2007-01-01

    Peanut (Arachis hypogaea) allergy is a major cause of food-induced anaphylaxis, with increasing prevalence worldwide. To date, there is no cure for peanut allergy, and, unlike many other food allergies, it usually persists through to adulthood. Prevention of exposure to peanuts is managed through strict avoidance, which can be compromised by the frequent use of peanuts and peanut products in food preparations. Conventional subcutaneous-injection allergen immunotherapy using crude peanut extract is not a recommended treatment because of the risk of severe side effects, largely as a result of specific IgE antibodies. Consequently, there is an urgent need to develop a suitable peanut allergen preparation that can induce specific clinical and immunological tolerance to peanuts in allergic individuals without adverse side effects. This requires detailed molecular and immunological characterisation of the allergenic components of peanut. This article reviews current knowledge on clinically relevant peanut allergens, in particular Ara h 1, Ara h 2 and Ara h 3, together with options for T-cell-reactive but non-IgE-binding allergen variants for specific immunotherapeutic strategies. These include T-cell-epitope peptide and hypoallergenic mutant vaccines. Alternative routes of administration such as sublingual are also considered, and appropriate adjuvants for delivering effective treatments at these sites examined. PMID:17210088

  16. Differential Induction of Flavonoids in Groundnut in Response to Helicoverpa armigera and Aphis craccivora Infestation

    Science.gov (United States)

    War, Abdul Rashid; Sharma, Suraj Prasad; Sharma, Hari Chand

    2016-01-01

    Flavonoids are important plant secondary metabolites, which protect plants from various stresses, including herbivory. Plants differentially respond to insects with different modes of action. High performance liquid chromatography (HPLC) fingerprinting of phenols of groundnut (Arachis hypogaea) plants with differential levels of resistance was carried out in response to Helicoverpa armigera (chewing insect) and Aphis craccivora (sucking pest) infestation. The genotypes used were ICGV 86699, ICGV 86031, ICG 2271 (NCAc 343), ICG 1697 (NCAc 17090), and JL 24. Most of the identified compounds were present in H. armigera- and A. craccivora-infested plants of ICGV 86699. Syringic acid was observed in all the genotypes across the treatments, except in the uninfested control plants of ICG 2271 and aphid-infested plants of ICG 1697. Caffeic acid and umbelliferone were observed only in the H. armigera-infested plants of ICGV 86699. Similarly, dihydroxybenzoic acid and vanillic acid were observed in H. armigera- and aphid-infested plants of ICG 2271 and JL 24, respectively. The peak areas were transformed into the amounts of compounds by using internal standard peak areas and were expressed in nanograms. Quantities of the identified compounds varied across genotypes and treatments. The common compounds observed were chlorogenic, syringic, quercetin, and ferulic acids. These results suggest that depending on the mode of feeding, flavonoids are induced differentially in groundnut plants. PMID:27398031

  17. Effects of interactions of auxin-producing bacteria and bacterial-feeding nematodes on regulation of peanut growths.

    Science.gov (United States)

    Xu, Li; Xu, Wensi; Jiang, Ying; Hu, Feng; Li, Huixin

    2015-01-01

    The influences of an IAA (indole-3-acetic acid)-producing bacterium (Bacillus megaterium) and two bacterial-feeding nematodes (Cephalobus sp. or Mesorhabditis sp.) on the growth of peanut (Arachis hypogaea L. cv. Haihua 1) after various durations of time were investigated in natural soils. The addition of bacteria and nematodes and incubation time all significantly affected plant growth, plant root growth, plant nutrient concentrations, soil nutrient concentrations, soil microorganisms and soil auxin concentration. The addition of nematodes caused greater increases in these indices than those of bacteria, while the addition of the combination of bacteria and nematodes caused further increases. After 42-day growth, the increases in soil respiration differed between the additions of two kinds of nematodes because of differences in their life strategies. The effects of the bacteria and nematodes on the nutrient and hormone concentrations were responsible for the increases in plant growth. These results indicate the potential for promoting plant growth via the addition of nematodes and bacteria to soil. PMID:25867954

  18. Effects of interactions of auxin-producing bacteria and bacterial-feeding nematodes on regulation of peanut growths.

    Directory of Open Access Journals (Sweden)

    Li Xu

    Full Text Available The influences of an IAA (indole-3-acetic acid-producing bacterium (Bacillus megaterium and two bacterial-feeding nematodes (Cephalobus sp. or Mesorhabditis sp. on the growth of peanut (Arachis hypogaea L. cv. Haihua 1 after various durations of time were investigated in natural soils. The addition of bacteria and nematodes and incubation time all significantly affected plant growth, plant root growth, plant nutrient concentrations, soil nutrient concentrations, soil microorganisms and soil auxin concentration. The addition of nematodes caused greater increases in these indices than those of bacteria, while the addition of the combination of bacteria and nematodes caused further increases. After 42-day growth, the increases in soil respiration differed between the additions of two kinds of nematodes because of differences in their life strategies. The effects of the bacteria and nematodes on the nutrient and hormone concentrations were responsible for the increases in plant growth. These results indicate the potential for promoting plant growth via the addition of nematodes and bacteria to soil.

  19. Gene expression profiling during seed-filling process in peanut with emphasis on oil biosynthesis networks.

    Science.gov (United States)

    Gupta, Kapil; Kayam, Galya; Faigenboim-Doron, Adi; Clevenger, Josh; Ozias-Akins, Peggy; Hovav, Ran

    2016-07-01

    Pod-filling is an important stage of peanut (Arachis hypogaea) seed development. It is partially controlled by genetic factors, as cultivars considerably vary in pod-filling potential. Here, a study was done to detect changes in mRNA levels that accompany pod-filling processes. Four seed developmental stages were sampled from two peanut genotypes differing in their oil content and pod-filling potential. Transcriptome data were generated by RNA-Seq and explored with respect to genic and subgenomic patterns of expression. Very dynamic transcriptomic changes occurred during seed development in both genotypes. Yet, general higher expression rates of transcripts and an enrichment in processes involved "energy generation" and "primary metabolites" were observed in the genotype with the better pod-filling ("Hanoch"). A dataset of 584 oil-related genes was assembled and analyzed, resulting in several lipid metabolic processes highly expressed in Hanoch, including oil storage and FA synthesis/elongation. Homoeolog-specific gene expression analysis revealed that both subgenomes contribute to the oil genes expression. Yet, biases were observed in particular parts of the pathway with possible biological meaning, presumably explaining the genotypic variation in oil biosynthesis and pod-filling. This study provides baseline information and a resource that may be used to understand development and oil biosynthesis in the peanut seeds. PMID:27181953

  20. IDIOSYNCRASY OF LOCAL FUNGAL ISOLATE HYPOCREA RUFA STRAIN P2: PLANT GROWTH PROMOTION AND MYCOPARASITISM

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    Parth Thakor

    2016-06-01

    Full Text Available Trichoderma viride an anamorph of Hypocrea rufa, is a known bio-control agent against various fungal phytopathogens. In the present study, H. rufa strain P2 was tested for plant growth promoting (PGP traits and antifungal activity against Fusarium oxysporum, Alternaria alternata, Aspergillus niger, Sclerotium rolfsii. In-vitro assessment of H. rufa strain P2 showed maximum IAA production of 68 µg ml-1, solubilised tri-calcium phosphate up to 72 µg ml-1 and showed production of chitinase enzyme 120 U ml-1. In order to determine in-vivo plant growth promotion, talc based formulation of H. rufa strain P2 was prepared and tested on Arachis hypogaea L. using seed and soil application. After 15 days, treated plants showed six-fold increases in the fresh and dry root mass whereas, fresh and dry shoot mass was increased up to two folds. The result indicates the local isolate H. rufa strain P2 can be categorized as phyto-friendly fungi which can be used as both, bio-control agent as well as phyto-augmenting bio-fertilizer.

  1. Resistance to Aspergillus flavus in maize and peanut:Molecular biology, breeding, environmental stress,and future perspectives

    Institute of Scientific and Technical Information of China (English)

    Jake; C.Fountain; Pawan; Khera; Liming; Yang; Spurthi; N.Nayak; Brian; T.Scully; Robert; D.Lee; Zhi-Yuan; Chen; Robert; C.Kemerait; Rajeev; K.Varshney; Baozhu; Guo

    2015-01-01

    The colonization of maize(Zea mays L.) and peanut(Arachis hypogaea L.) by the fungal pathogen Aspergillus flavus results in the contamination of kernels with carcinogenic mycotoxins known as aflatoxins leading to economic losses and potential health threats to humans. The regulation of aflatoxin biosynthesis in various Aspergillus spp. has been extensively studied, and has been shown to be related to oxidative stress responses. Given that environmental stresses such as drought and heat stress result in the accumulation of reactive oxygen species(ROS) within host plant tissues, host-derived ROS may play an important role in cross-kingdom communication between host plants and A. flavus. Recent technological advances in plant breeding have provided the tools necessary to study and apply knowledge derived from metabolomic, proteomic, and transcriptomic studies in the context of productive breeding populations. Here, we review the current understanding of the potential roles of environmental stress, ROS, and aflatoxin in the interaction between A.flavus and its host plants, and the current status in molecular breeding and marker discovery for resistance to A. flavus colonization and aflatoxin contamination in maize and peanut. We will also propose future directions and a working model for continuing research efforts linking environmental stress tolerance and aflatoxin contamination resistance in maize and peanut.

  2. Resistance to Aspergillus flavus in maize and peanut:Molecular biology, breeding, environmental stress, and future perspectives

    Institute of Scientific and Technical Information of China (English)

    Jake C. Fountain; Baozhu Guo; Pawan Khera; Liming Yang; Spurthi N. Nayak; Brian T. Scully; Robert D. Lee; Zhi-Yuan Chen; Robert C. Kemerait; Rajeev K. Varshney

    2015-01-01

    The colonization of maize (Zea mays L.) and peanut (Arachis hypogaea L.) by the fungal pathogen Aspergillus flavus results in the contamination of kernels with carcinogenic mycotoxins known as aflatoxins leading to economic losses and potential health threats to humans. The regulation of aflatoxin biosynthesis in various Aspergillus spp. has been extensively studied, and has been shown to be related to oxidative stress responses. Given that environmental stresses such as drought and heat stress result in the accumulation of reactive oxygen species (ROS) within host plant tissues, host-derived ROS may play an important role in cross-kingdom communication between host plants and A. flavus. Recent technological advances in plant breeding have provided the tools necessary to study and apply knowledge derived from metabolomic, proteomic, and transcriptomic studies in the context of productive breeding populations. Here, we review the current understanding of the potential roles of environmental stress, ROS, and aflatoxin in the interaction between A. flavus and its host plants, and the current status in molecular breeding and marker discovery for resistance to A. flavus colonization and aflatoxin contamination in maize and peanut. We will also propose future directions and a working model for continuing research efforts linking environmental stress tolerance and aflatoxin contamination resistance in maize and peanut.

  3. Influence of Cultural and Pest Management Practices on Performance of Runner, Spanish, and Virginia Market Types in North Carolina

    Directory of Open Access Journals (Sweden)

    Bridget R. Lassiter

    2016-01-01

    Full Text Available Virginia market type peanut (Arachis hypogaea L. cultivars are grown primarily in North Carolina, South Carolina, and Virginia in the US, although growers in these states often plant other market types if marketing opportunities are available. Information on yield potential and management strategies comparing these market types is limited in North Carolina. In separate experiments, research was conducted to determine response of runner, Spanish, and Virginia market types to calcium sulfate and inoculation with Bradyrhizobium at planting, planting and digging dates, planting patterns, and seeding rates. In other experiments, control of thrips (Frankliniella spp. using aldicarb, southern corn rootworm (Diabrotica undecimpunctata Howardi using chlorpyrifos, eclipta (Eclipta prostrata L. using threshold-based postemergence herbicides, and leaf spot disease (caused by the fungi Cercospora arachidicola and Cercosporidium personatum fungicide programs was compared in these market types. Results showed that management practice and market types interacted for peanut pod yield in only the planting date experiment. Yield of runner and Virginia market types was similar and exceeded yield of the Spanish market type in most experiments.

  4. Utilization of induced mutations for groundnut breeding in Uganda

    International Nuclear Information System (INIS)

    Groundnuts (Arachis hypogaea L.) are on high demand in Uganda. There is, therefore, an urgent need to improve groundnut yields through breeding. The main objectives besides yield are the following: 1. To improve disease resistance: (a) rosette virus transmitted by aphids (Aphis craccivora); (b) leafspot caused by Cercospora arachidicola (early) and Cercosporidium personatum (late). 2. To advance the maturity period of high yielding varieties so as to fit better into the rainfall pattern of the main growing areas. 3. To improve seed uniformity, seed size and quality (protein, oil). 4. To reduce plant height by shortening the internodes so as to have more flower production near the ground. For mutation breeding three erect groundnut cultivars were used, Roxo a recommended commercial variety; Red Beauty (Bl) a recommended local variety and No. 534 a tan skinned variety. Seeds of the three varieties were irradiated in 1976 at the FAO/IAEA Agricultural Section of the IAEA Laboratory Seibersdorf, with 1500 rad of fast neutrons (Nf) or 20 krad of 60Co gamma rays. The pedigree method of selection was used until M9. During 1985 and 1986, seven mutant selections of Red Beauty and one from Roxo were tested in replicated yield trials. Results are given. On the basis of plot yields some of the Red Beauty mutant lines outyielded the parent but not the commercial variety Roxo

  5. Utilization of induced mutations in groundnuts in Uganda

    International Nuclear Information System (INIS)

    Three cultivars of groundnut (Arachis hypogaea L.) were treated with two different types of ionizing radiation. These were gamma rays, at a dose of 20 krad, and fast neutrons, at a dose of 1500 rad, irradiating mature seeds. Several mutants were obtained from the treated material. Major visible changes observed included an increased number of branches per plant, increase/reduction in plant size, change in stem colour, change in plant growth habit and improvement in seed quality. Chlorophyll mutations were also observed and the affected albino plants died within two weeks of germination. Change in flower colour, from normal yellow to white, was observed in the M3 generation involving 37 plants. Mutants showing beneficial characters, such as high pod yield, good pod and seed quality, early maturity and disease resistance, were selected. Single plant selection was carried out up to the ninth generation, when preliminary yield trials (replicated) commenced for the more promising mutant lines. Results for eight mutants and the two original parents are given for one season. (author). 15 refs, 4 tabs

  6. Studies on soil aeration with irrigation and response of crops

    International Nuclear Information System (INIS)

    The root zone of the plant must be well supplied with both water and oxygen. Water potential should be kept close to saturation but if a low water tension is maintained, particularly in clay soil, plants will suffer most of the time from a suboptimal level of oxygen supply in the root zone. The diffusion rate of gases in the air is about 10,000 times greater than in water. Thus, it is obvious that the rate of gas diffusion decreases as the water content of the soil increases. These two requirements are apparently contradictory and the assessment of optimum level of soil aeration in the root environment is essential for better crop establishment and growth. Influence of irrigation treatments (three depths of irrigation of 2, 4 and 6 cm and four IW/CPE ratios of 0.45, 0.60, 0.75 and 0.90) on the soil oxygen diffusion rate (ODR) during various growing phases of peanut crop (Arachis hypogaea L.) were studied on a lateritic sandy loam soil (ultisols) for two consecutive seasons. The rate of ODR decreases as water content of the soil increases after irrigation and then increases gradually with the lapse of time. However, it starts decreasing with decrease in soil moisture after 96 hours of irrigation. (author)

  7. Physicochemical properties, fatty acid profile and antioxidant activity of peanut oil

    International Nuclear Information System (INIS)

    The oil from seeds of 4 pea nut (Arachis hypogaea L.) varieties: Golden, Bari 2000, Mongphalla, and Mongphalli 334 cultivated in arid zones, was subjected to the comparative evaluation of its physicochemical properties, fatty acid profile and antioxidant activity. Pea nut seeds were found to be a rich source of crude fat (45.09-51.63 g/100 g dry weight). The physicochemical properties of the oil were investigated as specific gravity (0.915 +-0.008-0.918+-0.008), acid value (3.96+-0.22-4.95+-0.71 mg KOH/g oil), saponification value ( 226.40+-3.59-246.56+-2.04 mg KOH/g oil) and unsaponifiable matter (3.20 +- 0.23-4.20+-0.04 g/100 g oil). The higher amounts of unsaturated fatty acids (82.06-85.93%) were found to be present in each variety. A significant variation (p<0.05) was observed among the varieties regarding crude oil content, saponification value, oleic/linoleic (O/L) ratios, phenolic acid content and total antioxidant content. Golden was found to be high in oil content, O/L ratio, antioxidant profile and DPPH scavenging activity but low in iodine value. (author)

  8. Transgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    After pre-culture and treatment of osmosis,cotyledons of immature peanut(Arachis hypogaea L.)zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene.Selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/L hygromycin.Under continuous selection,hygromycin resistant plantlets were regenerated from somatic embryos and were recovered from nearly 1.6% of the bombarded cotyledons.The presence and integration of foreign DNA in regenerated hygromycin resistant plants was confirmed by PCR(polymerase chain reaction)for the intron-gus gene and by Southern hybridization of the hph gene.GUS enzyme activity was detected in leaflets from transgenic plants but not from control,non-transformed plants.The production of transgenic plants are mainly based on a newly improved somatic embryogenesis regeneration system developed by us.

  9. Plant growth promotion and root colonization by EPS producing Enterobacter sp. RZS5 under heavy metal contaminated soil.

    Science.gov (United States)

    Sayyed, R Z; Patel, P R; Shaikh, S S

    2015-02-01

    The heavy metal resistant bacterium isolated from field soil and identified as Enterobacter sp. RZS5 tolerates a high concentration (100-2000 μM) of various heavy metal ions such as Mn2+, Ni2+, Zn2+, Cu2+, CO2+ and Fe2+ when grown in such environment and produces exopolysaccharides (EPS). Here, we have demonstrated EPS production by Enterobacter sp. RZS5 during 60 h of growth in yeast extract mannitol broth (YEMB). The yield increased by two fold after the addition of 60 μM of Ca2+; 50 μM of Fe2+ and 60 μM of Mg2+ ions in YEMB, and the optimization of physico-chemical parameters. EPS was extracted with 30% (v/v) of isopropanol as against the commonly used 50% (v/v) isopropanol method. EPS-rich broth promoted seed germination, shoot height, root length, number of leaves and chlorophyll content of wheat (Triticum aestivum) seed and peanut (Arachis hypogaea) seed. The higher colony-forming unit of Enterobacter sp. in soil inoculated with EPS rich broth of Enterobacter sp. indicated the root colonizing potential and rhizosphere competence of the isolate. The FTIR spectra of the EPS extract confirmed the presence of the functional group characteristics of EPS known to exhibit a high binding affinity towards certain metal ions. This overall growth and vigour in plants along with the effective root colonization, reflected the potential of the isolate as an efficient bio-inoculant in bioremediation. PMID:25757243

  10. Effects of mixed energy particle field on embryonic leaflet culture and plant regeneration of peanut

    International Nuclear Information System (INIS)

    In order to study effects of mixed high energy particle field on embryonic leaflet culture and plant regeneration of peanut (Arachis hypogaea L.), dry seeds of peanut cultivar Luhua 11 were irradiated with five doses (0, 67, 105, 150 and 196 Gy), then the embryonic leaflets were separated and incubated on MSB5 induction media containing 10 mg/L 2, 4-D, and the subcultures were subsequently transferred to MSB5 media supplemented with 4 mg/L BAP. The results showed that the survival rate of explants decreased significantly with the increase of irradiation dose, except for that treated by 67 Gy. Somatic embryo induction rate also showed no significant difference between CK and 67 Gy, while that of 105 Gy and 150 Gy were much lower than that of 67 Gy. Nevertheless, there was no somatic embryo formation in 196 Gy. The frequencies of plant regeneration reduced remarkably with the increase of irradiation doses, except for that treated by 105 Gy and 150 Gy. According to the rate of survival explants, somatic embryo formation and plant regeneration, 105 Gy was the optimum mutation dose. The regenerated plantlets were grafted and transplanted to the field, and they eventually set seeds. (authors)

  11. Study of surface carbohydrates in Galba truncatula tissues before and after infection with Fasciola hepatica

    Science.gov (United States)

    Georgieva, Katya; Georgieva, Liliya; Mizinska-Boevska, Yana; Stoitsova, Stoyanka R

    2016-01-01

    The presence and distribution of surface carbohydrates in the tissues of Galba truncatula snails uninfected or after infection with Fasciola hepatica as well as on the surface of the snail-pathogenic larval stages of the parasite were studied by lectin labelling assay. This is an attempt to find similarities that indicate possible mimicry, utilised by the parasite as an evasion strategy in this snail-trematode system. Different binding patterns were identified on head-foot-mantle, hepatopancreas, genital glands, renopericardial complex of the host as well as of the snail-pathogenic larval stages of F. hepatica. The infection with F. hepatica leads to changes of labelling with Glycine max in the head-mantle cells and Arachis hypogaea in the tubular epithelium of the hepatopancreas. The lectin binding on the other snail tissues is not changed by the development of the larvae. Our data clearly demonstrated the similarity in labelling of G. truncatula tissues and the surface of the snail-pathogenic larval stages of F. hepatica. The role of glycosylation of the contact surfaces of both organisms in relation to the host-parasite interactions is also discussed. PMID:27384082

  12. Utilization of genetic variation created through induced mutations to develop drought tolerant groundnut mutants for the sandy rain-fed areas of western Sudan

    International Nuclear Information System (INIS)

    Groundnut (Arachis hypogaea. L.) is grown as a cash crop throughout the tropical and warm temperate regions of the world. Approximately 80% of the global production comes from developing countries and 67% of the total is produced in the seasonally rain fed areas of the semi-arid tropics (Gibbons, 1980). In Sudan, groundnut is grown under rain fed and irrigated sectors. Rain fed production accounts for 80% of the total production. Yield under the traditional rain fed farming conditions are very low (700kg/ha) compared to the world average (1200kg/ha). Low rainfall (250-450 mm) and short growing seasons (<90 days) are major constraints to groundnut production. Under these situations survival of the subsistence farmers depends entirely on minimizing the probabilities of crop failure. This can to some extent be addressed by adopting short term strategies of incorporating various physiological defense mechanisms into crop varieties to allow a certain level of realized yield in a more reliable manner (Subbarao et al, 1995)

  13. Analysis of expression and glycosylation of avian metapneumovirus attachment glycoprotein from recombinant baculoviruses.

    Science.gov (United States)

    Luo, Lizhong; Nishi, Krista; MacLeod, Erin; Sabara, Marta I; Li, Yan

    2010-11-01

    Recently, we reported the expression and glycosylation of avian metapneumovirus attachment glycoprotein (AMPV/C G protein) in eukaryotic cell lines by a transient-expression method. In the present study, we investigated the biosynthesis and O-linked glycosylation of the AMPV/C G protein in a baculovirus expression system. The results showed that the insect cell-produced G protein migrated more rapidly in SDS-PAGE as compared to LLC-MK2 cell-derived G proteins owing to glycosylation differences. The fully processed, mature form of G protein migrated between 78 and 86 kDa, which is smaller than the 110 kDa mature form of G expressed in LLC-MK2 cells. In addition, several immature G gene products migrating at 40-48 and 60-70 kDa were also detected by SDS-PAGE and represented glycosylated intermediates. The addition of the antibiotic tunicamycin, which blocks early steps of glycosylation, to insect cell culture resulted in the disappearance of two glycosylated forms of the G protein and identified a 38 kDa unglycosylated precursor. The maturation of the G protein was completely blocked by monensin, suggesting that the O-linked glycosylation of G initiated in the trans-Golgi compartment. The presence of O-linked sugars on the mature protein was further confirmed by lectin Arachis hypogaea binding assay. Furthermore, antigenic features of the G protein expressed in insect cells were evaluated by ELISA. PMID:20713098

  14. High relative humidity increases yield, harvest index, flowering, and gynophore growth of hydroponically grown peanut plants

    Science.gov (United States)

    Mortley, D. G.; Bonsi, C. K.; Loretan, P. A.; Hill, W. A.; Morris, C. E.

    2000-01-01

    Growth chamber experiments were conducted to study the physiological and growth response of peanut (Arachis hypogaea L.) to 50% and 85% relative humidity (RH). The objective was to determine the effects of RH on pod and seed yield, harvest index, and flowering of peanut grown by the nutrient film technique (NFT). 'Georgia Red' peanut plants (14 days old) were planted into growth channels (0.15 x 0.15 x 1.2 m). Plants were spaced 25 cm apart with 15 cm between channels. A modified half-Hoagland solution with an additional 2 mM Ca was used. Solution pH was maintained between 6.4 and 6.7, and electrical conductivity (EC) ranged between 1100 and 1200 microS cm-1. Temperature regimes of 28/22 degrees C were maintained during the light/dark periods (12 hours each) with photosynthetic photon flux (PPF) at canopy level of 500 micromoles-m-2s-1. Foliage and pod fresh and dry weights, total seed yield, harvest index (HI), and seed maturity were greater at high than at low RH. Plants grown at 85% RH had greater total and individual leaflet area and stomatal conductance, flowered 3 days earlier and had a greater number of flowers reaching anthesis. Gynophores grew more rapidly at 85% than at 50% RH.

  15. Resistance to Aspergillus flavus in maize and peanut: Molecular biology, breeding, environmental stress, and future perspectives

    Directory of Open Access Journals (Sweden)

    Jake C. Fountain

    2015-06-01

    Full Text Available The colonization of maize (Zea mays L. and peanut (Arachis hypogaea L. by the fungal pathogen Aspergillus flavus results in the contamination of kernels with carcinogenic mycotoxins known as aflatoxins leading to economic losses and potential health threats to humans. The regulation of aflatoxin biosynthesis in various Aspergillus spp. has been extensively studied, and has been shown to be related to oxidative stress responses. Given that environmental stresses such as drought and heat stress result in the accumulation of reactive oxygen species (ROS within host plant tissues, host-derived ROS may play an important role in cross-kingdom communication between host plants and A. flavus. Recent technological advances in plant breeding have provided the tools necessary to study and apply knowledge derived from metabolomic, proteomic, and transcriptomic studies in the context of productive breeding populations. Here, we review the current understanding of the potential roles of environmental stress, ROS, and aflatoxin in the interaction between A. flavus and its host plants, and the current status in molecular breeding and marker discovery for resistance to A. flavus colonization and aflatoxin contamination in maize and peanut. We will also propose future directions and a working model for continuing research efforts linking environmental stress tolerance and aflatoxin contamination resistance in maize and peanut.

  16. Effect of Trichoderma harzianum biomass and Bradyrhizobium sp. strain NC 92 to control leaf blight disease of bambara groundnut (Vigna subterranea caused by Rhizoctonia solani in the field

    Directory of Open Access Journals (Sweden)

    Mana Kanjanamaneesathian

    2007-01-01

    Full Text Available Four hundred and sixty two strains of Trichoderma spp. were isolated from 23 soil samples in which groundnut (Arachis hypogaea L. and bambara groundnut (Vigna subterranea L. had been planted in Songkhla, Phattalung, Nakhon Si Thammarat, Narathiwat and Yala provinces. These fungi were tested against Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut, using dual culture technique on PDA medium. Among 462 isolates tested, 226 isolates had an ability to overgrow R. solani completely. Further testing found 13 isolates having the ability to parasitize mycelia of R. solani. Among these isolates, ThB-1-54 produced a cellulolytic enzyme on congo-red agar. This isolate was later identified as T. harzianum Rifai. In the field test, applying biomass of the isolate ThB-1-54 cultured on ground mesocarp fiber of oil palm, the combination of the isolate ThB-1-54 on ground mesocarp fiber of oil palm and Bradyrhizobium sp. (strain NC 92, or fungicide (iprodione had no effect on disease severity, yield, or the amount of total nitrogen content in stems or seeds of bambara groundnut plant.

  17. Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut

    International Nuclear Information System (INIS)

    The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm−3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10–50 Gy) or treated with 1–5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm−3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm−3) and 0.5 mg dm−3 2,4-D for maturation. The well-developed embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm−3 BAP and 0.25 mg dm−3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape. (author)

  18. EFFECT OF SHADING ON THE GROWTH AND PHOTOSYNTHESIS OF ARACHIS PIN TOI%遮荫对多年生花生(Arachis pintoi)生长的影响

    Institute of Scientific and Technical Information of China (English)

    彭建宗; 陈兆平

    1999-01-01

    多年生花生在全光照至80%遮荫度的环境中都能生长.随着遮荫度的增大,其叶片面积和叶绿素含量均明显增加,净光合速率、呼吸速率和光补偿点均下降,20%遮荫度的株鲜重与全光照下没有变化,80%遮荫度使株鲜重降低了36%.

  19. Effects of Ground and Unground Rice Straw on the Yield and Proximate Composition of Groundnut (Arachis hypogeae L.

    Directory of Open Access Journals (Sweden)

    Abdul'Aziz AYINLA

    2016-06-01

    Full Text Available A field experiment was carried out to evaluate the influence of ground and unground rice straw application as organic manure on the yield and proximate composition of groundnut seeds, at the University of Ilorin teaching and research farm. The field layout was a split plot Complete Randomized Block Design with four replications. The ground and unground rice straw were in the main plot and different application rates of the rice straw (0; 1,250; 2,500; 3,750 and 5,000 kg /ha were in the subplots. The results revealed that ground and unground rice straws at 1,250 and 2,500 kg/ha application rates significantly increased (p < 0.05 yield attributes such as pods’ number with a range of 48.33-74.33 pod weight (23.95-42.70, number of seeds (45.67-77.33, seeds’ weight (13.55-25.56 g and hundred seed weight (34.34-38.05 g as well as some aspect of proximate composition such as ash content, crude protein and crude fats which ranged between 2.71-2.79%, 31.47-31.56% and 46.61-47.13% respectively, in order of their mention. The forgoing study revealed that ground and unground rice straw applied at the rate of 1,250 and 2,500 kg/ha can serve as an alternative for soil nutrient amendment in groundnut as it ensured a reasonable yield that is able to meet the nutritional needs of man and livestock.

  20. Groundnut improvement: use of genetic and genomic tools.

    Science.gov (United States)

    Janila, Pasupuleti; Nigam, S N; Pandey, Manish K; Nagesh, P; Varshney, Rajeev K

    2013-01-01

    Groundnut (Arachis hypogaea L.), a self-pollinated legume is an important crop cultivated in 24 million ha world over for extraction of edible oil and food uses. The kernels are rich in oil (48-50%) and protein (25-28%), and are source of several vitamins, minerals, antioxidants, biologically active polyphenols, flavonoids, and isoflavones. Improved varieties of groundnut with high yield potential were developed and released for cultivation world over. The improved varieties belong to different maturity durations and possess resistance to diseases, tolerance to drought, enhanced oil content, and improved quality traits for food uses. Conventional breeding procedures along with the tools for phenotyping were largely used in groundnut improvement programs. Mutations were used to induce variability and wide hybridization was attempted to tap variability from wild species. Low genetic variability has been a bottleneck for groundnut improvement. The vast potential of wild species, reservoir of new alleles remains under-utilized. Development of linkage maps of groundnut during the last decade was followed by identification of markers and quantitative trait loci for the target traits. Consequently, the last decade has witnessed the deployment of molecular breeding approaches to complement the ongoing groundnut improvement programs in USA, China, India, and Japan. The other potential advantages of molecular breeding are the feasibility to target multiple traits for improvement and provide tools to tap new alleles from wild species. The first groundnut variety developed through marker-assisted back-crossing is a root-knot nematode-resistant variety, NemaTAM in USA. The uptake of molecular breeding approaches in groundnut improvement programs by NARS partners in India and many African countries is slow or needs to be initiated in part due to inadequate infrastructure, high genotyping costs, and human capacities. Availability of draft genome sequence for diploid (AA and BB

  1. 杜氏盐藻磷酸烯醇式丙酮酸羧化酶基因的克隆和分析%Cloning and analysis of phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina

    Institute of Scientific and Technical Information of China (English)

    张楠楠; 潘卫东; 崔玉琳; 秦松; 薛乐勋

    2011-01-01

    为研究杜氏盐藻(Dunaliella salina)磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)基因的功能, 根据莱茵衣藻(Chlamydomonas reinhardtii)、拟南芥(Arabidopsis thaliana)、花生(Arachis hypogaea)等真核生物PEPC 基因高度保守序列, 设计一对简并引物, 通过RT-PCR 的方法获得杜氏盐藻PEPC 基因部分序列, 然后采用RACE 的方法分别克隆到5′端和3′端序列, 拼接后得到全长cDNA, 其长度为3 523bp, 包含2 949 bp 的完整开放阅读框, 编码982 个氨基酸, 相对分子质量为110560.5。氨基酸序列与已知物种PEPC 序列的同源性依次为: Chlamydomonas reinhardtii 69%, Chlorellavariabilis 55%, Ostreococcus tauri 50%和Ostreococcus lucimarinus CCE9901 49%, 表明所克隆的序列确为杜氏盐藻PEPC cDNA 序列。%To investigate the function of phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina, a pair of degenerate primers was designed according to the conserved motifs of the PEPC of Chlamydomonas reinhardtii, Arabidopsis thaliana, and Arachis hypogaea. A cDNA fragment was obtained from green alga Dunaliella salina through RT-PCR, and then the full length of the cDNA was isolated by 3’ and 5’RACE. The isolated cDNA sequence was 3523 bp in length with a 2949 bp coding region that encoded 982 amino acid residues with the predicted relative molecular mass of 110560.5 dolton. In addition, homology analysis showed that PEPC of D. salina was highly similar to that of C. reinhardtii(69%), Chlorella variabilis(55%), Ostreococcus tauri(50%), and O. lucimarinus CCE9901(49%), suggesting that the cDNA isolated from Dunaliella salina was PEPC-encoding.

  2. Effect of replacing dietary soybean meal with tropical legumes on the performance of lambs

    International Nuclear Information System (INIS)

    This study determined how supplementing bahiagrass hay (Paspalum notatum Flugge cv. Pensacola) with soybean [Glycine max (L.) Merr.] meal or warm-season legume hays affects intake, digestibility, and N utilization by lambs. Forty-two Dorper x Katadhin crossbred lambs (30.6 ± 5.5 kg) were fed ad libitum 6-wk regrowth bahiagrass hay (73.8% NDF, 8.1% CP) supplemented with nothing (Control), soybean meal, or hays of annual peanut [Arachis hypogaea (L.) cv. Florida MDR98; 46.2% NDF, 14.7% CP], cowpea [Vigna unguiculata (L.) Walp. cv. Iron clay; 62.2% NDF, 11.7% CP], perennial peanut (Arachis glabrata Benth. cv. Florigraze; 43.3% NDF, 15.2% CP), pigeonpea [Cajanus cajan (L.) Millsp. cv. Georgia two; 78.6% NDF, 12.2% CP], or soybean (cv. Pioneer 97B52; 59.0% NDF, 13.5% CP). Legume hays were supplemented at 50% of total diet DM and soybean meal was supplemented at a level (4.25% of diet DM) that matched the average dietary CP content (10.8%) of the legume hay-supplemented diets. The pulses were harvested at respective maturities that maximized both DM yield and nutritive value, and the peanuts were first cuttings. Diets were fed to six lambs per treatment for two consecutive 21-d periods. Supplementation with hays of annual and perennial peanut, cowpea, and soybean increased (P ≤ 0.002) DMI, but apparent DM digestibility was only increased (P ≤ 0.03) by supplementation with annual or perennial peanut hay. Nitrogen intake, digestibility, and retention were increased (P < 0.001) by all supplements and responses were greater when annual or perennial peanut hays were fed. Ruminal ammonia concentration was increased (P ≤ 0.01) by all legume hay supplements. Microbial N synthesis and ruminally-degraded OM were increased (P ≤ 0.03) by perennial and annual peanut supplementation, but efficiency of microbial synthesis was not different (P ≥ 0.52) among diets. Annual and perennial peanuts were the most promising legume hay supplements for the lambs followed by cowpea and

  3. 木薯间套作栽培研究概况及产业发展对策%Research survey on cassava intercropping cultivation and the countermeasures for developing cassava industry

    Institute of Scientific and Technical Information of China (English)

    严炜; 刘光华; 娄予强; 刘倩; 李月仙; 郭容琦; 段春芳

    2011-01-01

    Cassava is recognized worldwidely as a great potential crop as a source of renewable energy. Its derivatives have a great development potential in national and international markets. The paper systematically describes the advantages, cultivation patterns and methods of cassava intercropping with different crops (Arachis hypogaea, Glycine max,Benincasa hispida, Citrullus lanatus, Zea mays, Volvariella volvacea, Arachis pintoi, and woody perennials), and the economic benefits of intercropping of cassava in recent years. Also the problem associated with cassava industry and their feasible suggestions and solutions have been discussed. The suggestions include the reinforcement of related policies,financial support for agriculture and personal with ability, guaranteed healthy development of cassava industry, quicken fund provision, new variety breeding and cultivation technique extension, development of cassava leading enterprises,exertion of energetic support to the driving functions of dragon-head corporations and promotion of the social-economic development.%木薯是世界公认的一种极具发展潜力的可再生能源作物,其已开发的衍生产品在国内外市场具有极大的发展潜力.文章系统介绍了木薯间套作栽培模式、效益和优势以及我国木薯产业中存在的主要问题,提出加强对木薯产业的政策、资金、人才投入,为木薯产业发展提供保障,积极引进、选育和推广木薯新品种与丰产栽培技术,推动木薯产业持续健康发展,延长木薯产业链,大力培育龙头企业,发挥龙头企业带动效应,推动区域经济发展等建议.

  4. Potencial produtivo de linhagens de amendoim do grupo ereto precoce com e sem controle de doenças foliares Yield potential of upright early maturing peanut lines with and without control of foliar diseases

    Directory of Open Access Journals (Sweden)

    IGNÁCIO JOSÉ DE GODOY

    2001-01-01

    Full Text Available Avaliaram-se o potencial produtivo, o rendimento de grãos após descascamento e a massa de grãos de onze linhagens de amendoim (Arachis hypogaea L. do programa de melhoramento do Instituto Agronômico e da cultivar IAC-Tatu-ST, com e sem controle de doenças foliares. O material genético classifica-se no grupo ereto precoce (A. hypogaea subsp. fastigiata, Waldron. Seis experimentos foram desenvolvidos na época das águas, no Núcleo de Agronomia da Alta Mogiana e na Estação Experimental de Agronomia de Pindorama, em 1994/95, 1995/96 e 1996/97, em esquema com duas parcelas subdivididas, em blocos ao acaso com quatro repetições, em que as parcelas principais consistiram em tratamentos com e sem o controle químico das doenças. As parcelas tratadas foram pulverizadas periodicamente com 3,0 L.ha-1 do fungicida chlorothalonil para controle da mancha-castanha e da mancha-preta (Cercospora arachidicola e Cercosporidium personatum e da verrugose (Sphaceloma arachidis. A ausência de controle químico resultou em significativas reduções, até de 60%, na produção de todos os genótipos. Com as doenças controladas, as linhagens IAC 5, IAC 21, IAC 22 e IAC 81-12 apresentaram maior potencial produtivo, atingindo 5.000 kg.ha-1 em dois dos experimentos. As linhagens IAC 81-7 e IAC 88-1 apresentaram os melhores desempenhos relativos entre as médias de ambientes com e sem controle químico. As doenças causaram reduções até de 14% no peso médio dos grãos, sobretudo nas linhagens IAC 5, IAC 21 e IAC 22, cujos grãos são de maior tamanho médio. Não foram observadas diferenças significativas em rendimento de grãos entre os tratamentos, na maioria dos ambientes; essa característica mostrou, também, ser pouco influenciada pela presença das doenças.Eleven peanut lines and the cultivar IAC-Tatu-ST, of the upright early maturing group (Arachis hypogae subsp. fastigiata, Waldron, were evaluated for pod yield, shelling percentage and average

  5. Efeito da mucuna e amendoim em rotação com algodoeiro A study on crop rotation for cotton using velvet bean and peanut

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    Carlos A. M. Ferraz

    1977-01-01

    Full Text Available O efeito da rotação de mucuna (Stizolobium atterrimum Piper & Tracy e amendoim (Arachis hypogaea L. e de duas variedades comerciais de algodoeiro, IAC RM3 e IAC 12-2 (Gossypium hirsutum L. foi estudado nos anos agrícolas de 1967/68 a 1972/73. Foram instalados dois ensaios, um em Presidente Bernardes, com fusariose, em solo podzolizado de Lins e Marília var. Lins naturalmente infectado por Fusarium oxysporumf. vasinfectum e o nematóide causador de galhas Meloidogyne incognita (Kofoid & White Chitwood, e outro em Presidente Venceslau, sem fusariose, em latossolo vermelho-escuro f. arenosa não infectado. A variedade comercial IAC RM3 é resistente e a IAC 12-2 é suscetível à fusariose. Para a análise estatística dos dados adotou-se o esquema de parcelas subdivididas, com seis repetições, tendo sido consideradas como parcelas as variedades de algodoeiro IAC RM3 e IAC 12-2, plantadas em 1968/69, 1970/71, 1971/72 e 1972/73, e como subparcelas as culturas em rotação, mucuna, amendoim e as variedades de algodoeiro IAC RM3 e IAC 12-2, plantadas nos anos-agrícolas de 1967/68 e 1969/70. Em solos com fusariose, em 1968/69, e em solos sem fusariose, no ano agrícola de 1970/71, destacou-se o efeito da rotação com mucuna, seguida da rotação com amendoim. Depois do plantio consecutivo de algodoeiro durante três anos (1970/71 a 1972/73, cessaram praticamente os efeitos da rotação para os dois casos. Houve aumento do teor de potássio após o primeiro ano de rotação, sendo maior para a mucuna.The effect of rotation of velvet bean (Stizolobium atterrimum Piper & Tracy, and peanut (Arachis hypogaea L. with two comercial varieties of cotton IAC RM3 and IAC 12-2 (Gossypium hirsutum L. was studied during 1967/68 to 1972/73. One experiment was conducted in a soil naturally infected by Fusarium oxysporum f. vasinfectum (Atk. Snyder & Hansen and by Meloidogyne incognita (Kofoid & White Chitwood, (President Bernardes, State of São Paulo, in

  6. Capacidade fotossintética de genótipos de amendoim em ambiente natural e controlado Photosynthetic capacity of peanut genotypes under natural and controlled environment

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    Norma de Magalhães Erismann

    2006-07-01

    Full Text Available A capacidade fotossintética das cultivares de amendoim rasteiro (Arachis hypogaea L. IAC-Caiapó e Runner IAC-886 foi avaliada sob condição controlada, em plantas cultivadas em vasos, mantidos em casa de vegetação, e sob condição natural, em plantas irrigadas, cultivadas em tanques de alvenaria. A resposta da taxa de assimilação líquida de CO2 (A em decorrência da densidade de fluxo de fótons fotossinteticamente ativos (DFFF foi melhor em condição controlada, mas, nas duas condições, a mesma A máxima de ca. 28 µmol m-2 s-1 foi atingida. Em condição controlada, a saturação lumínica ocorreu próximo a 1.000 µmol m-2 s-1 , ao passo que sob condição natural, ocorreu em DFFF maiores. A temperatura foliar entre 23 e 36°C não afetou A. A diferença de pressão de vapor entre a folha e o ar causou o fechamento parcial dos estômatos, diminuindo A, quando acima de 3,0 kPa. As capacidades fotossintéticas das duas cultivares de amendoim foram iguais. Ambas cultivares apresentaram boa adaptação às variações diárias do ambiente, ocorridas durante o verão, apresentando fotoinibição dinâmica da fotossíntese no início da tarde (13-14h, manifestada pela queda reversível da eficiência quântica máxima (Fv/Fm do fotossistema II.Photosynthetic capacity of runner peanuts (Arachis hypogaea L. cv. IAC-Caiapó and cv. Runner IAC-886 was evaluated under controlled condition, in plants grown on pots maintained in a greenhouse, and in irrigated plants grown on soil-filled tanks made of concrete, and exposed to natural ambient condition. CO2 net assimilation rate (A response in relation to photosynthetic photon flux density (DFFF was better in controlled condition, but in both conditions the same maximum A of ca. 28 µmol m-2 s-1 was reached. Under controlled condition, light saturation was about 1,000 µmol m-2 s-1 , although under natural condition, saturation occurred at higher DFFF. Leaf temperature between 23 and 36°C did

  7. Suitability of peanut residue as a nitrogen source for a rye cover crop Resíduos da cultura de amendoim como fonte de nitrogênio para uma cultura de cobertura de centeio

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    Kipling Shane Balkcom

    2007-01-01

    Full Text Available Leguminous winter cover crops have been utilized in conservation systems to partially meet nitrogen (N requirements of succeeding summer cash crops, but the potential of summer legumes to reduce N requirements of a winter annual grass, used as a cover crop, has not been extensively examined. This study assessed the N contribution of peanut (Arachis hypogaea L. residues to a subsequent rye (Secale cereale L. cover crop grown in a conservation system on a Dothan sandy loam (fine-loamy, kaolinitic, thermic Plinthic Kandiudults at Headland, AL USA during the 2003-2005 growing seasons. Treatments were arranged in a split plot design, with main plots of peanut residue retained or removed from the soil surface, and subplots as N application rates (0, 34, 67 and 101 kg ha-1 applied in the fall. Peanut residue had minimal to no effect on rye biomass yields, N content, carbon (C /N ratio, or N, P, K, Ca and Zn uptake. Additional N increased rye biomass yield, and N, P, K, Ca, and Zn uptakes. Peanut residue does not contribute significant amounts of N to a rye cover crop grown as part of a conservation system, but retaining peanut residue on the soil surface could protect the soil from erosion early in the fall and winter before a rye cover crop grows sufficiently to protect the typically degraded southeastern USA soils.Culturas leguminosas de inverno tem sido utilizadas em sistemas conservacionistas para suprimento parcial das necessidades de nitrogênio (N de culturas subseqüentes de verão, mas o potencial destas culturas leguminosas de verão no sentido de reduzir as necessidades de N de gramíneas anuais de inverno, utilizadas como culturas de cobertura, ainda não foi extensivamente estudado. Este trabalho avaliou a contribuição dos resíduos de uma cultura de amendoim (Arachis hypogaea L. sobre as necessidades de N de uma cultura subsequente de centeio (Secale cereale L. como cobertura desenvolvida dentro de um sistema conservacionista, em um

  8. Effects of Aluminum Stress on Growth and Physiological Characteristics in Peanut Root%铝胁迫对花生根系生长和生理特性的影响

    Institute of Scientific and Technical Information of China (English)

    徐芬芬; 程诗雨; 田玉清

    2014-01-01

    In order to clarify the response of the peanut root to aluminum toxicity in acidic soil,the root growth and physiological characteristics of peanut (Arachis hypogaea L.)seedlings under different concentrations of aluminum were studied in this paper by hydroponics culture.The results showed that the growth of peanut seedling roots under the low concentrations of aluminum(0.5-1.0 mmol/L)was promoted at first nine days after aluminum treatment,the root length in 0.5,1.0 mmol/L aluminum treatment was improved by 9.6% and 16.6% respectively compared with CK,the root fresh weight in 0.5 ,1.0 mmol/L aluminum treatment was increased by 2.5% and 8.2% respectively compared with CK.The growth of peanut root in high concentrations of aluminum(≥2.0 mmol/L)was significantly inhibited.The root length and the root fresh weight in 8.0 mmol/L aluminum treatment were reduced by 45.0% and 26.5%respectively compared with CK at the first nine days after aluminum treatment.The SOD,POD activities of root in low concentrations of aluminum(0.5-1.0 mmol/L)were enhanced,and the root cell membrane permeability decreased,so the growth of peanut root was promoted.However, the SOD,POD activities of root in high concentrations of aluminum(≥2.0 mmol/L)decreased, and the root cell membrane permeability increased,so the growth of peanut root was inhibited.%为明确花生(Arachis hypogaea L.)根系对酸性土铝毒的反应,采用水培法研究了不同浓度铝条件下花生幼苗根系生长和生理特性的变化。结果表明:低浓度铝(0.5~1.0 mmol/L)可促进花生幼苗根系生长,至铝胁迫处理第9天,0.5、1.0 mmol/L铝胁迫处理根系长度分别较 CK(不添加铝)提高了9.6%、16.6%,根系鲜质量分别较 CK 提高了2.5%、8.2%;而高浓度铝(≥2.0 mmol/L)明显抑制花生根系的生长,至铝胁迫处理第9天,8.0 mmol/L铝胁迫处理根系长度、根系鲜质量分别较CK降低45.0%、26.5%。低浓度铝(0

  9. Envelhecimento acelerado e deterioração controlada em sementes de amendoim Accelerated aging and controlled deterioration of peanut seeds

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    Claudia Antonia Vieira Rossetto

    2004-08-01

    Full Text Available Pesquisas têm sido desenvolvidas visando determinar os procedimentos para a avaliação de diferenças no potencial fisiológico de lotes de sementes, com destaque aos testes de vigor. O objetivo deste trabalho foi estudar o envelhecimento acelerado e a deterioração controlada para avaliação do vigor de sementes de amendoim (Arachis hypogaea. Quatro lotes de sementes de amendoim da cultivar Tatu foram submetidos ao teste de envelhecimento acelerado, em caixas tipo gerbox, com temperatura de 42ºC ou 43ºC, por 48 horas e 72 horas, com e sem o emprego de solução saturada de NaCl, e ao teste de deterioração controlada, com teor de água de 15% e 20%, a 40ºC ou 45ºC, por 48 horas. O teor de água, a germinação e sanidade das sementes foram determinados. Pelos testes de germinação e de emergência, não houve diferença significativa de desempenho entre os quatro lotes. No teste de envelhecimento acelerado com solução salina, o período de 72 horas a 42ºC é suficiente para avaliar o potencial fisiológico das sementes. No teste de deterioração controlada, a combinação de 15% de teor de água nas sementes e 48 horas em banho maria a 45ºC é eficiente para detectar diferenças de vigor entre os lotes.Researches have been developed aiming to determine the procedures for physiological potential seeds evaluation with emphasis to vigour tests. The objective was to study the controlled deterioration and the accelerated aging in peanut (Arachis hypogaea seeds vigour. Four lots of peanut seeds were submitted to the ageing accelerated test, at 42ºC or 43ºC, for 48 hours and 72 hours, with or without the use of saturated solution of NaCl, and controlled deterioration test, with 15% and 20% water content, at 40ºC or 45ºC, for 48 hours. The evaluation of water content, germination and health test was accomplished. There was no different performance among the four lots by the germination and emergency tests. In the ageing accelerated

  10. Enzymatic activity and mineralization of carbon and nitrogen in soil cultivated with coffee and green manures Atividade enzimática e mineralização do carbono e nitrogênio sob solo cultivado com adubos verdes na cultura do cafeeiro

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    Elcio Liborio Balota

    2010-10-01

    Full Text Available There are great concerns about degradation of agricultural soils. It has been suggested that cultivating different plant species intercropped with coffee plants can increase microbial diversity and enhance soil sustainability. The objective of this study was to evaluate enzyme activity (urease, arylsulfatase and phosphatase and alterations in C and N mineralization rates as related to different legume cover crops planted between rows of coffee plants. Soil samples were collected in a field experiment conducted for 10 years in a sandy soil in the North of Paraná State, Brazil. Samples were collected from the 0-10 cm layer, both from under the tree canopy and in-between rows in the following treatments: control, Leucaena leucocephala, Crotalaria spectabilis, Crotalaria breviflora, Mucuna pruriens, Mucuna deeringiana, Arachis hypogaea and Vigna unguiculata. The soil was sampled in four stages of legume cover crops: pre-planting (September, after planting (November, flowering stage (February and after plant residue incorporation (April, from 1997 to 1999. The green manure species influenced soil enzyme activity (urease, arylsulfatase and phosphatase and C and N mineralization rates, both under the tree canopy and in-between rows. Cultivation of Leucaena leucocephala increased acid phosphatase and arilsulfatase activity and C and N mineralization both under the tree canopy and in-between rows. Intercropped L. leucocephala increased urease activity under the tree canopy while C. breviflora increased urease activity in-between rows.Existe grande preocupação sobre a degradação dos solos agrícolas. Tem sido sugerido que o cultivo de plantas intercalares no cafeeiro aumenta a diversidade microbiana e a sustentabilidade do solo. No presente trabalho foi avaliada a alteração na atividade de enzimas do solo (urease, arilsulfatase e fosfatase e na mineralização do C e N devido ao cultivo intercalar de diferentes leguminosas de verão na cultura do

  11. Características agronômicas do amendoinzeiro sob irrigação com águas salinas em solo com biofertilizantes = Agronomics Characteristicsof Peanuts under irrigation with saline water on soil with biofertilizers.

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    Geocleber Gomes de Sousa

    2012-08-01

    Full Text Available Objetivou-se com esse trabalho avaliar o efeito da salinidade da água de irrigação nas características agronômicas do amendoinzeiro (Arachis hypogaea L. cultivado em solo sem e com biofertilizantes. O experimento foi conduzido em estufa telada na Estação Agrometereológica, Campus do Pici, Fortaleza, CE. A semeadura foi feita em vasos utilizando-se, como substrato, um Argissolo Vermelho-Amarelo, com uma planta por vaso. O experimento obedeceu a um delineamento inteiramente casualizado, em esquema fatorial 4 x 3, com cinco repetições. Os fatores referem-se aos valores de condutividadeelétrica da água de irrigação: 1,5; 3,0; 4,5 e 6,0 dS m-1 e sem e com biofertilizantes (sem biofertilizante -B0; com biofertilizanteanaeróbico-B1; e com biofertilizante aeróbico - B2. Foram avaliadas as seguintes variáveis: pH, condutividade elétrica do solo, crescimento inicial em número de folhas, altura de plantas, diâmetro do colmo, área foliar e matéria seca da parte aérea. O biofertilizante bovino diminuiu os efeitos negativos das concentrações crescentes de sais na água de irrigação nas variáveis estudadas. O nível salino do solo foi maior na presença do biofertilizante anaeróbico. O biofertilizante anaeróbico foi mais eficiente que o aeróbico na redução dos efeitos depressivos dos sais das águas de irrigação às plantas.This study evaluated the effects of irrigation water salinity on agronomics characteristics of peanut (Arachis hypogaea L., cultivated without and with biofertilizers. The experiment was conducted in a greenhouse in the Estação Agrometereológica, Campus do Pici, Fortaleza, CE. The seeds were sown in pots using, as substrate, a Red-Yellow Argisol, with one plant per pot. The experiment followed a completely randomized design set as a 4 x 3 factorial, referring to four irrigation water electrical conductivity values: 1.5, 3.0, 4.5 and 6.0 dS m-1 in three soil configurations: B0(without biofertilizer, B1

  12. Threecornered alfalfa hopper (Hemiptera: Membracidae): seasonal occurrence, girdle distribution, and response to insecticide treatment on peanut in South Carolina.

    Science.gov (United States)

    Rahman, Khalidur; Bridges, William C; Chapin, Jay W; Thomas, James S

    2007-08-01

    A survey of threecornered alfalfa hopper, Spissistilus festinus (Say) (Hemiptera: Membracidae), damage in 60 South Carolina peanut, Arachis hypogaea L., fields showed that 89 and 58% of plants had feeding girdles during 2003 and 2004, respectively. Use of a foliar insecticide for other target pests reduced hopper damage. Hopper damage was not affected by sampling distance from the field edge; therefore, injury was adequately assessed at 10 m from field borders. In-furrow insecticide choice, planting date, soil texture, previous crop, or tillage did not measurably affect girdling. Subsequent field experiments demonstrated a cultivar effect on threecornered alfalfa hopper injury, with the standard runner-type cultivar ('Georgia Green') more susceptible than the standard Virginia-type ('NC-V11'). More than 50% of stem girdling occurred on the basal quarter (first five internodes) of the plant. Most feeding occurred on secondary branches of main and lateral stems. Weekly sampling of seven grower fields showed that adult hoppers colonize peanut during June and produce two generations on peanut. Only low levels of plant girding were observed in June, but plant girdling increased gradually through late July, when girdling markedly increased contemporary with peak populations of first generation nymphs and adults. A second increase in plant girdling, observed in early September, coincided with the second generation of nymphs on peanut. Foliar treatments at 45- 60 d after planting (DAP) were most effective in suppressing injury. Granular chlorpyrifos treatment also suppressed hopper injury. There was no yield response to insecticide treatments at the hopper injury levels in these tests (up to six girdles per plant). Although the economic injury level (EIL) for this pest has not been defined, our data indicate that a critical interval for monitoring hopper activity is the first 3 wk of July, before the occurrence of significant injury. Where growers have a consistent risk of

  13. Negative biomarker based male fertility evaluation: Sperm phenotypes associated with molecular-level anomalies

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    Peter Sutovsky

    2015-01-01

    Full Text Available Biomarker-based sperm analysis elevates the treatment of human infertility and ameliorates reproductive performance in livestock. The negative biomarker-based approach focuses on proteins and ligands unique to defective spermatozoa, regardless of their morphological phenotype, lending itself to analysis by flow cytometry (FC. A prime example is the spermatid specific thioredoxin SPTRX3/TXNDC8, retained in the nuclear vacuoles and superfluous cytoplasm of defective human spermatozoa. Infertile couples with high semen SPTRX3 are less likely to conceive by assisted reproductive therapies (ART and more prone to recurrent miscarriage while low SPTRX3 has been associated with multiple ART births. Ubiquitin, a small, proteolysis-promoting covalent posttranslational protein modifier is found on the surface of defective posttesticular spermatozoa and in the damaged protein aggregates, the aggresomes of spermiogenic origin. Semen ubiquitin content correlates negatively with fertility and conventional semen parameters, and with sperm binding of lectins LCA (Lens culinaris agglutinin; reveals altered sperm surface and PNA (Arachis hypogaea/peanut agglutinin; reveals acrosomal malformation or damage. The Postacrosomal Sheath WWI Domain Binding Protein (PAWP, implicated in oocyte activation during fertilization, is ectopic or absent from defective human and animal spermatozoa. Consequently, FC-parameters of PAWP correlate with ART outcomes in infertile couples and with fertility in bulls. Assays based on the above biomarkers have been combined into multiplex FC semen screening protocols, and the surface expression of lectins and ubiquitin has been utilized to develop nanoparticle-based bull semen purification method validated by field artificial insemination trials. These advances go hand-in-hand with the innovation of FC-technology and genomics/proteomics-based biomarker discovery.

  14. Characterization and compilation of polymorphic simple sequence repeat (SSR markers of peanut from public database

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    Zhao Yongli

    2012-07-01

    Full Text Available Abstract Background There are several reports describing thousands of SSR markers in the peanut (Arachis hypogaea L. genome. There is a need to integrate various research reports of peanut DNA polymorphism into a single platform. Further, because of lack of uniformity in the labeling of these markers across the publications, there is some confusion on the identities of many markers. We describe below an effort to develop a central comprehensive database of polymorphic SSR markers in peanut. Findings We compiled 1,343 SSR markers as detecting polymorphism (14.5% within a total of 9,274 markers. Amongst all polymorphic SSRs examined, we found that AG motif (36.5% was the most abundant followed by AAG (12.1%, AAT (10.9%, and AT (10.3%.The mean length of SSR repeats in dinucleotide SSRs was significantly longer than that in trinucleotide SSRs. Dinucleotide SSRs showed higher polymorphism frequency for genomic SSRs when compared to trinucleotide SSRs, while for EST-SSRs, the frequency of polymorphic SSRs was higher in trinucleotide SSRs than in dinucleotide SSRs. The correlation of the length of SSR and the frequency of polymorphism revealed that the frequency of polymorphism was decreased as motif repeat number increased. Conclusions The assembled polymorphic SSRs would enhance the density of the existing genetic maps of peanut, which could also be a useful source of DNA markers suitable for high-throughput QTL mapping and marker-assisted selection in peanut improvement and thus would be of value to breeders.

  15. Electrogenerated poly(pyrrole-lactosyl) and poly(pyrrole-3'-sialyllactosyl) interfaces: towards the impedimetric detection of lectins

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    Gondran, Chantal; Dubois, Marie-Pierre; Fort, Sebastien; Cosnier, Serge

    2013-07-01

    This paper reports on the impedimetric transduction of binding reaction between polymerized saccharides and target lectins. The controlled potential electro-oxidation of pyrrole-lactosyl and pyrrole-3’-sialyllactosyl at 0.95 V vs Ag/AgCl, provides thin and reproducible poly(pyrrole-saccharide) films. The affinity binding of two lectins: Arachis hypogaea, (PNA) and Maackia amurensis (MAA) onto poly(pyrrole-lactosyl) and poly(pyrrole-3’-sialyllactosyl) electrodes, was demonstrated by cyclic voltammetry in presence of ruthenium hexamine and hydroquinone. In addition, rotating disk experiments were carried out to determine the permeability of both polypyrrole films and its evolution after incubating with lectin target. Finally, the possibility of using the poly(pyrrole-lactosyl) or poly(pyrrole-3’-siallyllactosyl) films for the impedimetric transduction of the lectin binding reaction, was investigated with hydroquinone (2×10-3 mol L-1) as a redox probe in phosphate buffer. The resuting impedance spectra were interpreted and modeled as an equivalent circuit indicating that charge transfer resistance (Rct) and relaxation frequency (f°) parameters are sensitive to the lectin binding. Rct increases from 77 to 97 Ω cm2 for PNA binding and from 93 to 131 Ω cm2 for MAA binding. In parallel, f° decreases from 276 to 222 Hz for PNA binding and from 223 to 131 Hz for MAA binding. This evolution of both parameters reflects the steric hindrances generated by the immobilised lectins towards the permeation of the redox probe.

  16. Specificity analysis of lectins and antibodies using remodeled glycoproteins.

    Science.gov (United States)

    Iskratsch, Thomas; Braun, Andreas; Paschinger, Katharina; Wilson, Iain B H

    2009-03-15

    Due to their ability to bind specifically to certain carbohydrate sequences, lectins are a frequently used tool in cytology, histology, and glycan analysis but also offer new options for drug targeting and drug delivery systems. For these and other potential applications, it is necessary to be certain as to the carbohydrate structures interacting with the lectin. Therefore, we used glycoproteins remodeled with glycosyltransferases and glycosidases for testing specificities of lectins from Aleuria aurantia (AAL), Erythrina cristagalli (ECL), Griffonia simplicifolia (GSL I-B(4)), Helix pomatia agglutinin (HPA), Lens culinaris (LCA), Lotus tetragonolobus (LTA), peanut (Arachis hypogaeae) (PNA), Ricinus communis (RCA I), Sambucus nigra (SNA), Vicia villosa (VVA), and wheat germ (Triticum vulgaris) (WGA) as well as reactivities of anti-carbohydrate antibodies (anti-bee venom, anti-horseradish peroxidase [anti-HRP], and anti-Lewis(x)). After enzymatic remodeling, the resulting neoglycoforms display defined carbohydrate sequences and can be used, when spotted on nitrocellulose or in enzyme-linked lectinosorbent assays, to identify the sugar moieties bound by the lectins. Transferrin with its two biantennary complex N-glycans was used as scaffold for gaining diverse N-glycosidic structures, whereas fetuin was modified using glycosidases to test the specificities of lectins toward both N- and O-glycans. In addition, alpha(1)-acid glycoprotein and Schistosoma mansoni egg extract were chosen as controls for lectin interactions with fucosylated glycans (Lewis(x) and core alpha1,3-fucose). Our data complement and expand the existing knowledge about the binding specificity of a range of commercially available lectins. PMID:19123999

  17. Caractéristiques polliniques des plantes mellifères de la zone soudano-guinéenne d'altitude de l'ouest Cameroun

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    Pinta, JY.

    2008-01-01

    Full Text Available Pollen Characteristics of Melliferous Plants of the Soudano Guinean Western Highlands of Cameroon. Between November 2000 and 2001, an inventory and pollen characteristics study of major melliferous plants of the Menoua Division in the Western highlands of Cameroon (Latitude North 5° 21.45N- 5°35.44'N and Longitude east 10°04.72- 10°26.24 were carried out. A total of 78 melliferous plants belonging to 33 families were identified. In terms of number of plants, the most-represented species were Asteraceae (12.9%; Solanaceae (8.6%; Euphorbiaceae (7.6%; Myrtaceae and Malvaceae (6.4% respectively in decreasing order. As concerns pollen characteristics inter and intra families variations were recorded. The smallest pollen size (15.7 ± 1.6 μ was found with Leucaena leucocephala while Calliandra callothyrsus had the highest (190.9 ± 7.1 μ. Subcircular pollen form was predominant (Asteraceae 39.2% of the 78 melliferous plants followed respectively by spheric (20.3%; Convovulaceae, elliptic (12.2%; Dacryodes edulis, cordia sp., and triangular (10.8%; Myrtaceae. Melliferous plants with aperturated exine pollen (Ageratum conyzoides, Psidium guayava were predominant (71.7% compared to those without aperturated exine pollen (Manihot esculenta, Croton macrostachyus; 28.2%. Pollen ornamentation also showed a trend of variation between species. Smooth pollen plants (Arachis hypogaea, Psidium guajava were more numerous (46.1%, followed respectively by spined (25.6%; Asteracea, Malvaceae and scabrous pollen species (Casuarina equisetifolia, Musa paradisiaca.

  18. Generation of peanut mutants by fast neutron irradiation combined with in vitro culture

    International Nuclear Information System (INIS)

    Induced mutations have played an important role in the development of new plant varieties. In this study, we investigated the effects of fast neutron irradiation on somatic embryogenesis combined with plant regeneration in embryonic leaflet culture to develop new peanut (Arachis hypogaea L.) germplasm for breeding. The dry seeds of the elite cultivar Luhua 11 were irradiated with fast neutrons at dosages of 9.7, 14.0 and 18.0 Gy. The embryonic leaflets were separated and incubated in a medium with 10.0-mg/l 2,4-D to induce somatic embryogenesis. Next, they were incubated in a medium with 4.0-mg/l BAP for plant regeneration. As the irradiation dosage increased, the frequency of both somatic embryo formation and plantlet regeneration decreased. The regenerated plantlets were grafted onto rootstocks and were transplanted into the field. Later, the mature seeds of the regenerated plants were harvested. The M2 generation plants from most of the regenerated cultivars exhibited variations and segregation in vigor, plant height, branch and pod number, pod size, and pod shape. To determine whether the phenotypes were associated with genomic modification, we compared the DNA polymorphisms between the wild-type plants and 19 M3-generation individuals from different regenerated plants. We used 20 pairs of simple sequence repeat (SSR) primers and detected polymorphisms between most of the mutants and the wild-type plants (Luhua 11). Our results indicate that using a combination of fast neutron irradiation and tissue culture is an effective approach for creating new peanut germplasm. (author)

  19. Characterization of the N-glycans of female Angiostrongylus cantonensis worms.

    Science.gov (United States)

    Veríssimo, Carolina M; Morassutti, Alessandra L; von Itzstein, Mark; Sutov, Grigorij; Hartley-Tassell, Lauren; McAtamney, Sarah; Dell, Anne; Haslam, Stuart M; Graeff-Teixeira, Carlos

    2016-07-01

    Glycoconjugates play a crucial role in the host-parasite relationships of helminthic infections, including angiostrongyliasis. It has previously been shown that the antigenicity of proteins from female Angiostrongylus cantonensis worms may depend on their associated glycan moieties. Here, an N-glycan profile of A. cantonensis is reported. A total soluble extract (TE) was prepared from female A. cantonensis worms and was tested by western blot before and after glycan oxidation or N- and O-glycosidase treatment. The importance of N-glycans for the immunogenicity of A. cantonensis was demonstrated when deglycosylation of the TE with PNGase F completely abrogated IgG recognition. The TE was also fractionated using various lectin columns [Ulex europaeus (UEA), concanavalin A (Con A), Arachis hypogaea (PNA), Triticum vulgaris (WGA) and Lycopersicon esculentum (LEA)], and then each fraction was digested with PNGase F. Released N-glycans were analyzed with matrix-assisted laser desorption ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS) and MALDI-TOF/TOF-MS/MS. Complex-type, high mannose, and truncated glycan structures were identified in all five fractions. Sequential MALDI-TOF-TOF analysis of the major MS peaks identified complex-type structures, with a α1-6 fucosylated core and truncated antennas. Glycoproteins in the TE were labeled with BodipyAF558-SE dye for a lectin microarray analysis. Fluorescent images were analyzed with ProScanArray imaging software followed by statistical analysis. A total of 29 lectins showed positive binding to the TE. Of these, Bandeiraea simplicifolia (BS-I), PNA, and Wisteria floribunda (WFA), which recognize galactose (Gal) and N-acetylgalactosamine (GalNAc), exhibited high affinity binding. Taken together, our findings demonstrate that female A. cantonensis worms have characteristic helminth N-glycans. PMID:27107931

  20. Spatiotemporal patterns and dispersal of stink bugs (Heteroptera: Pentatomidae) in peanut-cotton farmscapes.

    Science.gov (United States)

    Tillman, P G; Northfield, T D; Mizell, R F; Riddle, T C

    2009-08-01

    In the southeast United States, a field of peanuts, Arachis hypogaea L., is often closely associated with a field of cotton, Gossypium hirsutum L. The objective of this 4-yr on-farm study was to examine and compare the spatiotemporal patterns and dispersal of the southern green stink bug, Nezara viridula L., and the brown stink bug, Euschistus servus (Say), in six of these peanut-cotton farmscapes. GS(+) Version 9 was used to generate interpolated estimates of stink bug density by inverse distance weighting. Interpolated stink bug population raster maps were constructed using ArcMap Version 9.2. This technique was used to show any change in distribution of stink bugs in the farmscape over time. SADIE (spatial analysis by distance indices) methodology was used to examine spatial aggregation of individual stink bug species and spatial association of the two stink bug species in the individual crops. Altogether, the spatiotemporal analyses for the farmscapes showed that some N. viridula and E. servus nymphs and adults that develop in peanuts disperse into cotton. When these stink bugs disperse from peanuts into cotton, they aggregate in cotton at the interface, or common boundary, of the two crops while feeding on cotton bolls. Therefore, there is a pronounced edge effect observed in the distribution of stink bugs as they colonize the new crop, cotton. The driving force for the spatiotemporal distribution and dispersal of both stink bug species in peanut-cotton farmscapes seems to be availability of food in time and space mitigated by landscape structure. Thus, an understanding of farmscape ecology of stink bugs and their natural enemies is necessary to strategically place, in time and space, biologically based management strategies that control stink bug populations while conserving natural enemies and the environment and reducing off-farm inputs. PMID:19689882

  1. Electrogenerated poly(pyrrole-lactosyl and poly(pyrrole-3’-sialyllactosyl interfaces: towards the impedimetric detection of lectins

    Directory of Open Access Journals (Sweden)

    Chantal eGondran

    2013-07-01

    Full Text Available This paper reports on the impedimetric transduction of binding reaction between polymerized saccharides and target lectins. The controlled potential electro-oxidation of pyrrole-lactosyl and pyrrole-3’-sialyllactosyl at 0.95 V vs Ag/AgCl, provides thin and reproducible poly(pyrrole-saccharide films. The affinity binding of two lectins: Arachis hypogaea, (PNA and Maackia amurensis (MAA onto poly(pyrrole-lactosyl and poly(pyrrole-3’-sialyllactosyl electrodes, was demonstrated by cyclic voltammetry in presence of ruthenium hexamine and hydroquinone. In addition, rotating disk experiments were carried out to determine the permeability of both polypyrrole films and its evolution after incubating with lectin target. Finally, the possibility of using the poly(pyrrole-lactosyl or poly(pyrrole-3’-siallyllactosyl films for the impedimetric transduction of the lectin binding reaction, was investigated with hydroquinone (2×10-3 mol L-1 as a redox probe in phosphate buffer. The resuting impedance spectra were interpreted and modeled as an equivalent circuit indicating that charge transfer resistance (Rct and relaxation frequency (f° parameters are sensitive to the lectin binding. Rct increases from 77 to 97 Ω cm2 for PNA binding and from 93 to 131 Ω cm2 for MAA binding. In parallel, f° decreases from 276 to 222 Hz for PNA binding and from 223 to 131 Hz for MAA binding. This evolution of both parameters reflects the steric hindrances generated by the immobilised lectins towards the permeation of the redox probe.

  2. Characterization of a Pinus pinaster cDNA encoding an auxin up-regulated putative peroxidase in roots.

    Science.gov (United States)

    Charvet-Candela, V; Hitchin, S; Reddy, M S; Cournoyer, B; Marmeisse, R; Gay, G

    2002-03-01

    As part of a study to identify host plant genes regulated by fungal auxin during ectomycorrhiza formation, we differentially screened a cDNA library constructed from roots of auxin-treated Pinus pinaster (Ait.) Sol. seedlings. We identified three cDNAs up-regulated by auxin. Sequence analysis of one of these cDNAs, PpPrx75, revealed the presence of an open reading frame of 216 amino acids with the characteristic consensus sequences of plant peroxidases. The deduced amino acid sequence showed homology with Arabidopsis thaliana (L.) Heynh., Arachis hypogaea L. and Stylosanthes humilis HBK cationic peroxidases. Amino acid sequence identities in the conserved domains of plant peroxidases ranged from 60 to 100%. In PpPrx75, there are five cysteine residues and one histidine residue that are found at conserved positions among other peroxidases. A potential glycosylation site (NTS) is present in the deduced sequence. Phylogenetic analysis showed that PpPrx75 is closely related to two A. thaliana peroxidases. The PpPrx75 cDNA was induced by active auxins, ethylene, abscisic acid and quercetin, a flavonoid possibly involved in plant-microorganism interactions. Transcript accumulation was detected within 3 h following root induction by auxin, and the amount of mRNA increased over the following 24 h. The protein synthesis inhibitor cycloheximide did not inhibit indole-3-acetic acid-induced transcript accumulation, suggesting that PpPrx75 induction is a primary (direct) response to auxin. This cDNA can be used to study expression of an auxin-regulated peroxidase during ectomycorrhiza formation. PMID:11874719

  3. Adenovirus-mediated expression of pig α(1, 3) galactosyltransferase reconstructs Gal α(1, 3) Gal epitope on the surface of human tumor cells

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Gal α(1,3)Gal(gal epitope)is a carbohydrate epitope and synthesized in large amount by α(1,3)galactosyltransferase [α(1,3)GT] enzyme on the cells of lower mammalian animals such as pigs and mice.Human has no gal epitope due to the inactivation of α(1,3)GT gene but produces a large amount of antibodies(anti-Gal)which recognize Gal α(1,3)Gal structures specifically.In this study,a replicationdeficient recombinant adenoviral vector Ad5sGT containing pig α(1,3)GT cDNA was constructed and characterized.Adenoviral vector-mediated transfer of pig α(1,3)GT gene into human tumor cells such as malignant melanoma A375,stomach cancer SGC-7901,and lung cancer SPC-A-1 was reported for the first time.Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis,although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after Ad5sGT transduction.Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I(UEA I)lectins,Vicia villosa agglutinin(VVA),Arachis hypogaea agglutinin(PNA),and Glycine max agglutinin(SBA)to different degrees.In addition,no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay.

  4. Characterization and Mineralization Rates of Low Temperature Peanut Hull and Pine Chip Biochars

    Directory of Open Access Journals (Sweden)

    K.C. Das

    2013-04-01

    Full Text Available Biochar can potentially increase soil fertility and sequester carbon by incorporating nutrients and stable black carbon into the soil; however its effect on soil nitrogen (N and carbon (C processes is not well understood. A defined methodology to characterize biochar is necessary to predict how specific biochars will affect C and N mineralization. We amended a Tifton soil (Fine-loamy, siliceous, thermic Plinthic Kandiudults with peanut hull (Arachis hypogaea; PH; 2.1% N and pine chip (Pinus taeda; PC: 0.4% N biochar at application rates of 1% and 2% (w/w and performed a 136-day mineralization study. A companion 24-day mineralization study amended Tifton soil with PH and PC biochar at 2% and their respective feedstocks at equal C rates. Soil C mineralization rates were monitored periodically throughout each study and total N mineralization rates were also measured. In addition, we characterized each biochar using thermogravimetric analysis with mass spectrometer (TGA-MS, proximate analysis, Fourier transform infrared spectroscopy (FTIR, and total mineral analysis to identify biochar characteristics that might correlate with mineralization properties. Limited C (<2% mineralized from both biochars, but mineralization rates of soil amended with PH biochar were higher than PC biochar. Carbon mineralization correlated well with estimated aliphatic content determined by TGA-MS but not with volatile content indicated by proximate analysis. Nitrogen was not mineralized from either biochar, indicating that plant-based biochar should not be considered a source of N for plant growth. The N in biochar may be contained in the stable aromatic structure of the biochar, as indicated by TGA-MS, and not available to soil microbes.

  5. Dietary plant lectins appear to be transported from the gut to gain access to and alter dopaminergic neurons of Caenorhabditis elegans, a potential etiology of Parkinson’s disease

    Directory of Open Access Journals (Sweden)

    Jolene eZheng

    2016-03-01

    Full Text Available Lectins from dietary plants have been shown to enhance drug absorption in the gastrointestinal tract of rats, be transported trans-synaptically as shown by tracing of axonal and dendritic paths, and enhance gene delivery. Other carbohydrate-binding protein toxins are known to traverse the gut intact in dogs. Post-feeding rhodamine- or TRITC-tagged dietary lectins, the lectins were tracked from gut to dopaminergic neurons (DAergic-N in transgenic Caenorhabditis elegans (C. elegans (egIs1[Pdat-1::GFP] where the mutant has the Green Fluorescent Protein (GFP gene fused to a dopamine transport protein gene labeling dopaminergic neurons, The lectins were supplemented along with the food organism Escherichia coli (OP50. Among nine tested rhodamine/TRITC-tagged lectins, four, including Phaseolus vulgaris erythroagglutinin (PHA-E, Bandeiraea simplicifolia (BS-I, Dolichos biflorus agglutinin (DBA, and Arachis hypogaea (PNA, appeared to be transported from gut to the GFP-DAergic-N. Griffonia Simplicifolia (GSL-I and PHA-E, reduced the number of GFP-DAergic-N suggesting a toxic activity. PHA-E, BS-I, Pisum Sativum (PSA, and Triticum vulgaris agglutinin (Succinylated reduced fluorescent intensity of GFP-DAergic-N. PHA-E, PSA, Concanavalin A, and Triticum vulgaris agglutinin decreased the size of GFP-DAergic-N, while BS-I increased neuron size. These observations suggest that dietary plant lectins are transported to and affect DAergic-N in C. elegans, which support Braak and Hawkes’ hypothesis, suggesting one alternate potential dietary etiology of Parkinson’s disease (PD. A recent Danish study showed that vagotomy resulted in 40% lower incidence of PD over 20 years. Differences in inherited sugar structures of gut and neuronal cell surfaces may make some individuals more susceptible in this conceptual disease etiology model.

  6. Exploratory use of a UAV platform for variety selection in peanut

    Science.gov (United States)

    Balota, Maria; Oakes, Joseph

    2016-05-01

    Variety choice is the most important production decision farmers make because high yielding varieties can increase profit with no additional production costs. Therefore, yield improvement has been the major objective for peanut (Arachis hypogaea L.) breeding programs worldwide, but the current breeding approach (selecting for yield under optimal production conditions) is slow and inconsistent with the needs derived from population demand and climate change. To improve the rate of genetic gain, breeders have used target physiological traits such as leaf chlorophyll content using SPAD chlorophyll meter, Normalized Difference Vegetation Index (NDVI) from canopy reflectance in visible and near infra-red (NIR) wavelength bands, and canopy temperature (CT) manually measured with infra-red (IR) thermometers at the canopy level; but its use for routine selection was hampered by the time required to walk hundreds of plots. Recent developments in remote sensing-based high throughput phenotyping platforms using unmanned aerial vehicles (UAV) have shown good potential for future breeding advancements. Recently, we initiated a study for the evaluation of suitability of digital imagery, NDVI, and CT taken from an UAV platform for peanut variety differentiation. Peanut is unique for setting its yield underground and resilience to drought and heat, for which yield is difficult to pre-harvest estimate; although the need for early yield estimation within the breeding programs exists. Twenty-six peanut cultivars and breeding lines were grown in replicated plots either optimally or deficiently irrigated under rain exclusion shelters at Suffolk, Virginia. At the beginning maturity growth stage, approximately a month before digging, NDVI and CT were taken with ground-based sensors at the same time with red, blue, green (RGB) images from a Sony camera mounted on an UAV platform. Disease ratings were also taken pre-harvest. Ground and UAV derived vegetation indices were analyzed for

  7. Dietary Plant Lectins Appear to Be Transported from the Gut to Gain Access to and Alter Dopaminergic Neurons of Caenorhabditis elegans, a Potential Etiology of Parkinson's Disease.

    Science.gov (United States)

    Zheng, Jolene; Wang, Mingming; Wei, Wenqian; Keller, Jeffrey N; Adhikari, Binita; King, Jason F; King, Michael L; Peng, Nan; Laine, Roger A

    2016-01-01

    Lectins from dietary plants have been shown to enhance drug absorption in the gastrointestinal tract of rats, be transported trans-synaptically as shown by tracing of axonal and dendritic paths, and enhance gene delivery. Other carbohydrate-binding protein toxins are known to traverse the gut intact in dogs. Post-feeding rhodamine- or TRITC-tagged dietary lectins, the lectins were tracked from gut to dopaminergic neurons (DAergic-N) in transgenic Caenorhabditis elegans (C. elegans) [egIs1(Pdat-1:GFP)] where the mutant has the green fluorescent protein (GFP) gene fused to a dopamine transport protein gene labeling DAergic-N. The lectins were supplemented along with the food organism Escherichia coli (OP50). Among nine tested rhodamine/TRITC-tagged lectins, four, including Phaseolus vulgaris erythroagglutinin (PHA-E), Bandeiraea simplicifolia (BS-I), Dolichos biflorus agglutinin (DBA), and Arachis hypogaea agglutinin (PNA), appeared to be transported from gut to the GFP-DAergic-N. Griffonia Simplicifolia and PHA-E, reduced the number of GFP-DAergic-N, suggesting a toxic activity. PHA-E, BS-I, Pisum sativum (PSA), and Triticum vulgaris agglutinin (Succinylated) reduced fluorescent intensity of GFP-DAergic-N. PHA-E, PSA, Concanavalin A, and Triticum vulgaris agglutinin decreased the size of GFP-DAergic-N, while BS-I increased neuron size. These observations suggest that dietary plant lectins are transported to and affect DAergic-N in C. elegans, which support Braak and Hawkes' hypothesis, suggesting one alternate potential dietary etiology of Parkinson's disease (PD). A recent Danish study showed that vagotomy resulted in 40% lower incidence of PD over 20 years. Differences in inherited sugar structures of gut and neuronal cell surfaces may make some individuals more susceptible in this conceptual disease etiology model. PMID:27014695

  8. Global Synthesis of Drought Effects on Food Legume Production.

    Science.gov (United States)

    Daryanto, Stefani; Wang, Lixin; Jacinthe, Pierre-André

    2015-01-01

    Food legume crops play important roles in conservation farming systems and contribute to food security in the developing world. However, in many regions of the world, their production has been adversely affected by drought. Although water scarcity is a severe abiotic constraint of legume crops productivity, it remains unclear how the effects of drought co-vary with legume species, soil texture, agroclimatic region, and drought timing. To address these uncertainties, we collected literature data between 1980 and 2014 that reported monoculture legume yield responses to drought under field conditions, and analyzed this data set using meta-analysis techniques. Our results showed that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with legume species and the phenological state during which drought occurred. Overall, lentil (Lens culinaris), groundnut (Arachis hypogaea), and pigeon pea (Cajanus cajan) were found to experience lower drought-induced yield reduction compared to legumes such as cowpea (Vigna unguiculata) and green gram (Vigna radiate). Yield reduction was generally greater when legumes experienced drought during their reproductive stage compared to during their vegetative stage. Legumes grown in soil with medium texture also exhibited greater yield reduction compared to those planted on soil of either coarse or fine texture. In contrast, regions and their associated climatic factors did not significantly affect legume yield reduction. In the face of changing climate, our study provides useful information for agricultural planning and research directions for development of drought-resistant legume species to improve adaptation and resilience of agricultural systems in the drought-prone regions of the world. PMID:26061704

  9. Bradyrhizobium kavangense sp. nov., a symbiotic nitrogen-fixing bacterium from root nodules of traditional Namibian pulses.

    Science.gov (United States)

    Lasse Grönemeyer, Jann; Hurek, T; Reinhold-Hurek, Barbara

    2015-12-01

    Eight strains of symbiotic bacteria from root nodules of local races of cowpea (Vigna unguiculata) and Bambara groundnut (Vigna subterranea) grown on subsistence farmers' fields in the Kavango region, Namibia, were previously characterized and identified as a novel group within the genus Bradyrhizobium. To clarify their taxonomic status, these strains were further characterized using a polyphasic approach. In phylogenetic analysis of the 16S rRNA gene sequence the novel group was most closely related to Bradyrhizobium iriomotense EK05T and Bradyrhizobium ingae BR 10250T, and to 'Bradyrhizobium arachidis' CCBAU 051107 in the ITS sequence analysis. Phylogenetic analysis of concatenated glnII-recA-rpoB-dnaK sequences placed the strains in a lineage distinct from named species of the genus Bradyrhizobium. The species status was validated by results of DNA-DNA hybridization. Phylogenetic analysis of nifH and nodC genes placed the novel strains in a group with 'B. arachidis' CCBAU 051107. The combination of phenotypic characteristics from several tests including carbon source utilization and antibiotic resistance could be used to differentiate representative strains from recognized species of the genus Bradyrhizobium. Novel strain 14-3T induces effective nodules on Vigna subterranea, Vigna unguiculata, Arachis hypogaea and Lablab purpureus. Based on the data presented, it is concluded that the strains represent a novel species of the genus Bradyrhizobium, for which the name Bradyrhizobium kavangense sp. nov. is proposed. The type strain is 14-3T [ = DSM 100299T = LMG 28790T = NTCCM 0012T (Windhoek)]. The DNA G+C content of strain 14-3T is 63.8 mol% (Tm). PMID:26446190

  10. Lectin binding and effects in culture on human cancer and non-cancer cell lines: examination of issues of interest in drug design strategies.

    Science.gov (United States)

    Petrossian, Karineh; Banner, Lisa R; Oppenheimer, Steven B

    2007-01-01

    By using a non-cancer and a cancer cell line originally from the same tissue (colon), coupled with testing lectins for cell binding and for their effects on these cell lines in culture, this study describes a simple multi-parameter approach that has revealed some interesting results that could be useful in drug development strategies. Two human cell lines, CCL-220/Colo320DM (human colon cancer cells, tumorigenic in nude mice) and CRL-1459/CCD-18Co (non-malignant human colon cells) were tested for their ability to bind to agarose microbeads derivatized with two lectins, peanut agglutinin (Arachis hypogaea agglutinin, PNA) and Dolichos biflorus agglutinin (DBA), and the effects of these lectins were assessed in culture using the MTT assay. Both cell lines bound to DBA-derivatized microbeads, and binding was inhibited by N-acetyl-D-galactosamine, but not by L-fucose. Neither cell line bound to PNA-derivatized microbeads. Despite the lack of lectin binding using the rapid microbead method, PNA was mitogenic in culture at some time points and its mitogenic effect displayed a reverse-dose response. This was also seen with effects of DBA on cells in culture. While this is a simple study, the results were statistically highly significant and suggest that: (1) agents may not need to bind strongly to cells to exert biological effects, (2) cell line pairs derived from diseased and non-diseased tissue can provide useful comparative data on potential drug effects and (3) very low concentrations of potential drugs might be initially tested experimentally because reverse-dose responses should be considered. PMID:17706752

  11. Chlorophyll fluorescence induction kinetics and yield responses in rainfed crops with variable potassium nutrition in K deficient semi-arid alfisols.

    Science.gov (United States)

    Srinivasarao, Ch; Shanker, Arun K; Kundu, Sumanta; Reddy, Sharanbhoopal

    2016-07-01

    Optimum potassium (K) nutrition in semi-arid regions may help crop plants to overcome constraints in their growth and development such as moisture stress, leading to higher productivity of rainfed crops, thus judicious K management is essential. A study was conducted to evaluate the importance of K nutrition on physiological processes like photosynthesis through chlorophyll a fluorescence and chlorophyll fluorescence induction kinetics (OJIP) of rainfed crops viz., maize (Zea mays L.), pearl millet (Pennisetum glaucum), groundnut (Arachis hypogaea), sunflower (Helianthus annuus), castor (Ricinus communis L.) and cotton (Gossypium hirsutum) under water stress conditions by studying their growth attributes, water relations, yield, K uptake and use efficiency under varied K levels. Highest chlorophyll content was observed under K60 in maize and pearl millet. Narrow and wide Chl a:b ratio was observed in castor and groundnut respectively. The fluorescence yield decreased in the crops as K dosage increased, evidenced by increasing of all points (O, J, I and P) of the OJIP curves. The fluorescence transient curve for K60 was lower than K0 and K40 for all the crops. Potassium levels altered the fluorescence induction and impaired photosynthetic systems in all the crops studied. There was no distinct trend observed in leaf water potential of crops under study. Uptake of K was high in sunflower with increased rate of K application. Quantitatively, K uptake by castor crop was lesser compared to all other crops. Our results indicate that the yield reduction under low K was due to the low capacity of the crops to translocate K from non-photosynthetic organs such as stems and petioles to upper leaves and harvested organs and this in turn influenced the capacity of the crops to produce a high economic yield per unit of K taken up thus reducing utilization efficiency of K. PMID:27101276

  12. Combined selection and multivariate analysis in early generations of intraspecific progenies of peanuts

    Directory of Open Access Journals (Sweden)

    Lucas Nunes da Luz

    2014-03-01

    Full Text Available Selection indexes are useful tools for the simultaneous selection of several descriptors aiming to promote genetic gains and a reliable advancement of segregating populations. This study tested the use of selection index of distance from the genotype to the ideotype to assist in the search for superior progenies, based on agronomic variables obtained from early generation progeny of intraspecific progenies of peanuts (Arachis hypogaea L. Four segregating populations were used, each consisting of 90 progenies, arranged in a randomized block design with three replicates, in which the best 30 progenies of each population were selected. The index of distance to the ideotype, used for the selection, was built from a non-real model cultivar that meets the requirements for traits main stem height (SH, early flowering (EF, number of pegs (NP, number of pods per plant (NPP, weight of 100 pods (WP100, number of seeds per plant, and weight 100 seed (WS100. It was observed that the selection index used was effective for differentiating progenies and promoting gains at a selection pressure of 33.33%. Gains were variable for the same traits in the different populations. The number of pods per plant, for example, ranged from 3.96% in the population 3 to 16.98% in the population 4. The analysis of diversity showed that progenies selected grouped differently, by principal coordinates analysis, which corroborates the ability of the index to form groups according to traits of interest. The groups formed revealed moderate variability by the diversity analysis by the Ward/MLM method.

  13. Novel and Stress Relevant EST Derived SSR Markers Developed and Validated in Peanut.

    Directory of Open Access Journals (Sweden)

    Tejas C Bosamia

    Full Text Available With the aim to increase the number of functional markers in resource poor crop like cultivated peanut (Arachis hypogaea, large numbers of available expressed sequence tags (ESTs in the public databases, were employed for the development of novel EST derived simple sequence repeat (SSR markers. From 16424 unigenes, 2784 (16.95% SSRs containing unigenes having 3373 SSR motifs were identified. Of these, 2027 (72.81% sequences were annotated and 4124 gene ontology terms were assigned. Among different SSR motif-classes, tri-nucleotide repeats (33.86% were the most abundant followed by di-nucleotide repeats (27.51% while AG/CT (20.7% and AAG/CTT (13.25% were the most abundant repeat-motifs. A total of 2456 EST-SSR novel primer pairs were designed, of which 366 unigenes having relevance to various stresses and other functions, were PCR validated using a set of 11 diverse peanut genotypes. Of these, 340 (92.62% primer pairs yielded clear and scorable PCR products and 39 (10.66% primer pairs exhibited polymorphisms. Overall, the number of alleles per marker ranged from 1-12 with an average of 3.77 and the PIC ranged from 0.028 to 0.375 with an average of 0.325. The identified EST-SSRs not only enriched the existing molecular markers kitty, but would also facilitate the targeted research in marker-trait association for various stresses, inter-specific studies and genetic diversity analysis in peanut.

  14. Plants with potential use on obesity and its complications.

    Science.gov (United States)

    Gamboa-Gómez, Claudia I; Rocha-Guzmán, Nuria E; Gallegos-Infante, J Alberto; Moreno-Jiménez, Martha R; Vázquez-Cabral, Blanca D; González-Laredo, Rubén F

    2015-01-01

    Obesity is the most prevalent nutritional disease and a growing public health problem worldwide. This disease is a causal component of the metabolic syndrome related with abnormalities, including hyperglycemia, dyslipidemia, hypertension, inflammation, among others. There are anti-obesity drugs, affecting the fundamental processes of the weight regulation; however they have shown serious side effects, which outweigh their beneficial effects. Most recent studies on the treatment of obesity and its complications have focused on the potential role of different plants preparation that can exert a positive effect on the mechanisms involved in this pathology. For instance, anti-obesity effects of green tea and its isolated active principles have been reported in both in vitro (cell cultures) and in vivo (animal models) that possess healthy effects, decreasing adipose tissue through reduction of adipocytes differentiation and proliferation. A positive effect in lipid profile, and lipid and carbohydrates metabolisms were demonstrated as well. In addition, anti-inflammatory and antioxidant activities were studied. However, the consumption of green tea and its products is not that common in Western countries, where other plants with similar bioactivity predominate; nevertheless, the effect extension has not been analyzed in depth, despite of their potential as alternative treatment for obesity. In this review the anti-obesity potential and reported mechanisms of action of diverse plants such as: Camellia sinensis, Hibiscus sabdariffa, Hypericum perforatum, Persea americana, Phaseolus vulgaris, Capsicum annuum, Rosmarinus officinalis, Ilex paraguariensis, Citrus paradisi, Citrus limon, Punica granatum, Aloe vera, Taraxacum officinale and Arachis hypogaea is summarized. We consider the potential of these plants as natural alternative treatments of some metabolic alterations associated with obesity. PMID:26869866

  15. Impacts of Type of Fallow and Invasion by Chromolaena odorata on Weed Communities in Crop Fields in Cameroon

    Directory of Open Access Journals (Sweden)

    Morag McDonald

    2004-12-01

    Full Text Available In the humid forest regions of southern Cameroon in central Africa, sectoral and macroeconomic policy reforms introduced in the late 1980s have led to intensified land use, which in turn has resulted in, among other environmental consequences, shortened fallow systems dominated by the Asteraceae shrub, Chromolaena odorata (L. King and Robinson, rather than by secondary forest species. A trial was established to determine the effect of shortened fallow duration and invasion by C. odorata on the weed flora in subsequent mixed food cropping systems. Plots were established in cleared 5- to 7-year-old fallow fields in which the vegetation was either dominated by C. odorata or not, and in which the dominant fallow vegetation in the previous crop–fallow rotation had been either C. odorata, forest, or herbaceous (not dominated by C. odorata. Cassava (Manihot esculenta Crantz, maize (Zea mays L., and groundnuts (Arachis hypogaea L. were intercropped and weed species were assessed 6, 14, and 30 weeks after crop planting. Soil analyses were conducted to assess the influence of edaphic traits on the distribution and abundance of dominant weed species. The results clearly indicated an enrichment of the weed flora with time after planting, but little difference between fallow histories. Two groups of weed species corresponded with soil characteristics: C. odorata, Cyathula prostrata, Mariscus alternifolius, Mikania cordata, Musanga cecropioides, and Trema orientalis were preponderant on soils with high clay, N, and C contents, and Ageratum conyzoides, Cyperus sp., Haumania danckelmaniana, Paspalum conjugatum, Pouzolzia guineensis, Richardia brasiliensis, Sida rhombifolia, Stachytarpheta cayennensis, Talinum triangulare, and Triumfetta cordifolia were preponderant on sandier soils with high pH, P, and Mg contents.

  16. Global Synthesis of Drought Effects on Food Legume Production.

    Directory of Open Access Journals (Sweden)

    Stefani Daryanto

    Full Text Available Food legume crops play important roles in conservation farming systems and contribute to food security in the developing world. However, in many regions of the world, their production has been adversely affected by drought. Although water scarcity is a severe abiotic constraint of legume crops productivity, it remains unclear how the effects of drought co-vary with legume species, soil texture, agroclimatic region, and drought timing. To address these uncertainties, we collected literature data between 1980 and 2014 that reported monoculture legume yield responses to drought under field conditions, and analyzed this data set using meta-analysis techniques. Our results showed that the amount of water reduction was positively related with yield reduction, but the extent of the impact varied with legume species and the phenological state during which drought occurred. Overall, lentil (Lens culinaris, groundnut (Arachis hypogaea, and pigeon pea (Cajanus cajan were found to experience lower drought-induced yield reduction compared to legumes such as cowpea (Vigna unguiculata and green gram (Vigna radiate. Yield reduction was generally greater when legumes experienced drought during their reproductive stage compared to during their vegetative stage. Legumes grown in soil with medium texture also exhibited greater yield reduction compared to those planted on soil of either coarse or fine texture. In contrast, regions and their associated climatic factors did not significantly affect legume yield reduction. In the face of changing climate, our study provides useful information for agricultural planning and research directions for development of drought-resistant legume species to improve adaptation and resilience of agricultural systems in the drought-prone regions of the world.

  17. Preliminary report on the tissue-culture of Arachis pintoi cv.Amarillo%平托花生组织培养研究初报

    Institute of Scientific and Technical Information of China (English)

    郑向丽; 黄毅斌; 徐国忠; 郑仲登

    2004-01-01

    对平托花生叶片、茎段、带腋芽茎段离体组织培养的研究表明,茎段培养21 d后逐渐褐化、枯死;带腋芽茎段在MS + 0.5 mg·L-1 NAA + 3.0 mg·L-1 6-BA培养基上可直接诱导出丛生芽;叶片在MS + 0.5 mg·L-1 NAA+ 3.0 mg·L-16-BA培养基上可成功诱导出愈伤组织, 进而再生出众多的芽并进一步伸长.分离的正常幼芽在MS + 0.5 mg·L-1 NAA培养基上培养15 d后长出不定根,并长成正常的平托花生植株.

  18. The manufacture of particleboards using mixture of peanut hull (Arachis hypoqaea L.) and European Black pine (Pinus nigra Arnold) wood chips.

    Science.gov (United States)

    Guler, Cengiz; Copur, Yalcin; Tascioglu, Cihat

    2008-05-01

    This research was conducted to investigate the suitability of peanut hull to produce general purpose particleboards. A series of panels were produced using peanut hull and mixture of peanut hull and European Black pine wood chips. Particleboards were manufactured using various hull ratios in the mixture (0%, 25%, 50%, 75% and 100%). Urea formaldehyde adhesive was utilized in board production and boards were produced to target panel's density of 0.7 g/cm3. Panels were tested for some physical (water absorption and thickness swelling), chemical (holocellulose content, lignin content, alcohol-benzene solubility, 1% NaOH solubility, hot water solubility and cold water solubility) and mechanical (modulus of rupture, modulus of elasticity and internal bond) properties. The main observation was that increase in peanut hull in the mixture resulted in a decrease in mechanical and physical properties of produced panels and panel including 25% hull in the mixture solely met the standard required by TS-EN 312 standard. Conclusively, a valuable renewable natural resource, peanut hull could be utilized in panel production while it has been mixed to the wood chips. PMID:17689074

  19. DREB1A promotes root development in deep soil layers and increases water extraction under water stress in groundnut.

    Science.gov (United States)

    Vadez, V; Rao, J S; Bhatnagar-Mathur, P; Sharma, K K

    2013-01-01

    Water deficit is a major yield-limiting factor for many crops, and improving the root system has been proposed as a promising breeding strategy, although not in groundnut (Arachis hypogaea L.). The present work was carried out mainly to assess how root traits are influenced under water stress in groundnut, whether transgenics can alter root traits, and whether putative changes lead to water extraction differences. Several transgenic events, transformed with DREB1A driven by the rd29 promoter, along with wild-type JL24, were tested in a lysimeter system that mimics field conditions under both water stress (WS) and well-watered (WW) conditions. The WS treatment increased the maximum rooting depth, although the increase was limited to about 20% in JL24, compared to 50% in RD11. The root dry weight followed a similar trend. Consequently, the root dry weight and length density of transgenics was higher in layers below 100-cm depth (Exp. 1) and below 30 cm (Exp. 2). The root diameter was unchanged under WS treatment, except a slight increase in the 60-90-cm layer. The root diameter increased below 60 cm in both treatments. In the WW treatment, total water extraction of RD33 was higher than in JL24 and other transgenic events, and somewhat lower in RD11 than in JL24. In the WS treatment, water extraction of RD2, RD11 and RD33 was higher than in JL24. These water extraction differences were mostly apparent in the initial 21 days after treatment imposition and were well related to root length density in the 30-60-cm layer (R(2) = 0.68), but not to average root length density. In conclusion, water stress promotes rooting growth more strongly in transgenic events than in the wild type, especially in deep soil layers, and this leads to increased water extraction. This opens an avenue for tapping these characteristics toward the improvement of drought adaptation in deep soil conditions, and toward a better understanding of genes involved in rooting in groundnut. PMID:22672619

  20. Efeitos de diferentes teores de umidade e espessuras do material de embalagem plástica na conservação de sementes de amendoim Effects of different moisture contents and thickness of the plastic packaging material on the preservation of peanut seeds

    Directory of Open Access Journals (Sweden)

    Romeu de Tella

    1979-01-01

    Full Text Available Determinaram-se, aos 3, 6, 9, 12 e 18 meses de armazenamento, as porcentagens de germinação e umidade de sementes de amendoim do cultivar tatu, descascadas mecanicamente, com níveis iniciais de 5,2; 6,2; 7,0; 8,2 e 9,2% de umidade, acondicionadas em sacos de polietileno de 0,05; 0,08; 0,10 e 0,15mm de espessura e mantidas em uma sala em condições não controladas de temperatura e umidade relativa, por um período de 18 meses. As umidades iniciais de 8,2 e 9,2% foram prejudiciais à conservação das sementes, principalmente quando estas foram acondicionadas nos sacos plásticos de 0,15 ou 0,10mm de espessura. As melhores condições para a manutenção da germinação das sementes foram a secagem aos níveis de 5,2 ou 6,2% de umidade e acondicionamento nos sacos plásticos de 0,15 ou 0,10mm de espessura. As paredes dos sacos plásticos não impediram trocas de umidade entre o ambiente e as sementes, sendo que essas trocas foram mais rápidas nos sacos de menor espessura.Mecanically shelled peanut (Arachis hypogaea L. seeds of the cultivar tatu, with initial moisture contents of 5.2, 6.2, 7.0, 8.2, and 9.2%, and packaged in polyethylene bags of 0.05, 0.08, 0.10, and 0.15mm thick. were stored in a room with no temperature and relative humidity control, in Campinas, State of São Paulo. Germination and moisture content percentagens were determined at 3, 6, 9, 12, and 18 months storage. The initial moisture contents of 8.2 and 9.2% were damaging to the preservation of the seeds, mainly when these were packaged in the bags of 0.15 or 0.10mm thick. The best storage conditions were those provided by drying to 5.2 or 6.2% moisture levels, and packaging in 0.15 or 0.10 mm thick plastic bags. The walls of the plastic bags did not hamper moisture vapor transmission between environment and seeds. The transmission rates were higher in the bags of thinner walls.

  1. A comparative study on the decomposition of edible and non-edible oil cakes in the Gangetic alluvial soil of West Bengal.

    Science.gov (United States)

    Mondal, Sudeshna; Das, Ritwika; Das, Amal Chandra

    2014-08-01

    An experiment has been conducted under laboratory conditions to investigate the effect of decomposition of two edible oil cakes, viz. mustard cake (Brassica juncea L) and groundnut cake (Arachis hypogaea L), and two non-edible oil cakes, viz. mahua cake (Madhuca indica Gmel) and neem cake (Azadirachta indica Juss), at the rate of 5.0 t ha(-1) on the changes of microbial growth and activities in relation to transformations and availability of some plant nutrients in the Gangetic alluvial (Typic Haplustept) soil of West Bengal, India. Incorporation of oil cakes, in general, highly induced the proliferation of total bacteria, actinomycetes, and fungi, resulting in greater retention and availability of oxidizable C, N, and P in soil. As compared to untreated control, the highest stimulation of total bacteria and actinomycetes was recorded with mustard cake (111.9 and 84.3 %, respectively) followed by groundnut cake (50.5 and 52.4 %, respectively), while the fungal colonies were highly accentuated due to the incorporation of neem cake (102.8 %) in soil. The retention of oxidizable organic C was highly increased due to decomposition of non-edible oil cakes, more so under mahua cake (14.5 %), whereas edible oil cakes and groundnut cake in particular exerted maximum stimulation (16.7 %) towards the retention of total N in soil. A similar trend was recorded towards the accumulation of available mineral N in soil and this was more pronounced with mustard cake (45.6 %) for exchangeable NH4 (+) and with groundnut cake (63.9 %) for soluble NO3 (-). The highest retention of total P (46.9 %) was manifested by the soil when it was incorporated with neem cake followed by the edible oil cakes; while the available P was highly induced due to the addition of edible oil cakes, the highest being under groundnut cake (23.5 %) followed by mustard cake (19.6 %). PMID:24733437

  2. NATURAL ANTIOXIDANT INGREDIENT FROM BY-PRODUCTS OF FRUITS

    Directory of Open Access Journals (Sweden)

    G. S. El-Baroty

    2014-01-01

    Full Text Available The contents of total phenolics compounds and their phenolic constituents were quantified in organic and aqueous of four varieties (Zebdia, Sukkari, taimor and Hindi of mango (Mangifera indica L., seeds pulp and kernel, one varieties of pomegranate (Punica ranatum L., peel and peanut (Arachis hypogaea L., Giza 6, shell by-products. The antioxidant activities of all by products extracts were assessed by five antioxidant methods as well as by rancimate test. The total Phenolic content of aqueous and organic extracts of among all mango varieties, pomegranate and peanut shell showed the content values ranging from 71.06 to 124.18 mg/100g, 95.07 to 124.18 mg/100g and 41.64 to 71.06, respectively. Nineteen phenolic compounds were identified by High Performance Liquid Chromatography (HPLC among all mango varieties, of which vanillic acid, benzoic acid and mangiferin were occurred in high amounts. The major phenolic compounds were detected in pomegranate and peanut shell were chlorogenic and gallic and caffeic (24.42%, respectively. All fruits by products were exhibited remarkable antioxidant activity, with various degrees in all tested methods. However, among all by-products extracts, organic extract had higher antioxidant than that aqueous extracts toward all antioxidant tested. Mango kernel peel and pomegranates showed high radical scavenging activity, which could be compared with the synthetic antioxidants Butylated Hydroxyanisol (BHA. However, all by-products extracts exhibited high inhibit effect against the lipid peroxidation of sunflower oil (at 100°C as assessed by rancimat methods. However, this antioxidant activity was found to be strong significant correlation with phenolic contents (p<0.05 in by-product extracts. It can be thus concluded that varied varieties of mango, pomegranate and peanut by-products, although it constitutes the part of the fruits, it is valuable parts due to its antioxidant activities, it can be

  3. Overexpression of Peanut Diacylglycerol Acyltransferase 2 in Escherichia coli

    Science.gov (United States)

    Yang, Lianqun; Zhang, Bin; Chen, Gao; Bi, Yuping

    2013-01-01

    Diacylglycerol acyltransferase (DGAT) is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the peanut cultivar ‘Luhua 14’ using a homologous gene sequence method and rapid amplification of cDNA ends. To understand the role of AhDGAT2 in triacylglycerol biosynthesis, two AhDGAT2 nucleotide sequences that differed by three amino acids were expressed as glutathione S-transferase (GST) fusion proteins in Escherichia coli Rosetta (DE3). Following IPTG induction, the isozymes (AhDGAT2a and AhDGAT2b) were expressed as 64.5 kDa GST fusion proteins. Both AhDGAT2a and AhDGAT2b occurred in the host cell cytoplasm and inclusion bodies, with larger amounts in the inclusion bodies. Overexpression of AhDGATs depressed the host cell growth rates relative to non-transformed cells, but cells harboring empty-vector, AhDGAT2a–GST, or AhDGAT2b–GST exhibited no obvious growth rate differences. Interestingly, induction of AhDGAT2a–GST and AhDGAT2b–GST proteins increased the sizes of the host cells by 2.4–2.5 times that of the controls (post-IPTG induction). The total fatty acid (FA) levels of the AhDGAT2a–GST and AhDGAT2a–GST transformants, as well as levels of C12:0, C14:0, C16:0, C16:1, C18:1n9c and C18:3n3 FAs, increased markedly, whereas C15:0 and C21:0 levels were lower than in non-transformed cells or those containing empty-vectors. In addition, the levels of some FAs differed between the two transformant strains, indicating that the two isozymes might have different functions in peanuts. This is the first time that a full-length recombinant peanut DGAT2 has been produced in a bacterial expression system and the first analysis of its effects on the content and composition of fatty acids in E. coli. Our results indicate that AhDGAT2 is a strong candidate gene for

  4. Overexpression of peanut diacylglycerol acyltransferase 2 in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Zhenying Peng

    Full Text Available Diacylglycerol acyltransferase (DGAT is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2 genes were cloned from the peanut cultivar 'Luhua 14' using a homologous gene sequence method and rapid amplification of cDNA ends. To understand the role of AhDGAT2 in triacylglycerol biosynthesis, two AhDGAT2 nucleotide sequences that differed by three amino acids were expressed as glutathione S-transferase (GST fusion proteins in Escherichia coli Rosetta (DE3. Following IPTG induction, the isozymes (AhDGAT2a and AhDGAT2b were expressed as 64.5 kDa GST fusion proteins. Both AhDGAT2a and AhDGAT2b occurred in the host cell cytoplasm and inclusion bodies, with larger amounts in the inclusion bodies. Overexpression of AhDGATs depressed the host cell growth rates relative to non-transformed cells, but cells harboring empty-vector, AhDGAT2a-GST, or AhDGAT2b-GST exhibited no obvious growth rate differences. Interestingly, induction of AhDGAT2a-GST and AhDGAT2b-GST proteins increased the sizes of the host cells by 2.4-2.5 times that of the controls (post-IPTG induction. The total fatty acid (FA levels of the AhDGAT2a-GST and AhDGAT2a-GST transformants, as well as levels of C12:0, C14:0, C16:0, C16:1, C18:1n9c and C18:3n3 FAs, increased markedly, whereas C15:0 and C21:0 levels were lower than in non-transformed cells or those containing empty-vectors. In addition, the levels of some FAs differed between the two transformant strains, indicating that the two isozymes might have different functions in peanuts. This is the first time that a full-length recombinant peanut DGAT2 has been produced in a bacterial expression system and the first analysis of its effects on the content and composition of fatty acids in E. coli. Our results indicate that AhDGAT2 is a strong candidate gene for

  5. Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies

    Indian Academy of Sciences (India)

    Rajeev K Varshney; Himabindu Kudapa; Manish Roorkiwal; Mahendar Thudi; Manish K Pandey; Rachit K Saxena; Siva K Chamarthi; Murali Mohan S; Nalini Mallikarjuna; Hari Upadhyaya; Pooran M Gaur; L Krishnamurthy; K B Saxena; Shyam N Nigam; Suresh Pande

    2012-11-01

    Molecular markers are the most powerful genomic tools to increase the efficiency and precision of breeding practices for crop improvement. Progress in the development of genomic resources in the leading legume crops of the semi-arid tropics (SAT), namely, chickpea (Cicer arietinum), pigeonpea (Cajanus cajan) and groundnut (Arachis hypogaea), as compared to other crop species like cereals, has been very slow. With the advances in next-generation sequencing (NGS) and high-throughput (HTP) genotyping methods, there is a shift in development of genomic resources including molecular markers in these crops. For instance, 2,000 to 3,000 novel simple sequence repeats (SSR) markers have been developed each for chickpea, pigeonpea and groundnut. Based on Sanger, 454/FLX and Illumina transcript reads, transcriptome assemblies have been developed for chickpea (44,845 transcript assembly contigs, or TACs) and pigeonpea (21,434 TACs). Illumina sequencing of some parental genotypes of mapping populations has resulted in the development of 120 million reads for chickpea and 128.9 million reads for pigeonpea. Alignment of these Illumina reads with respective transcriptome assemblies have provided > 10,000 SNPs each in chickpea and pigeonpea. A variety of SNP genotyping platforms including GoldenGate, VeraCode and Competitive Allele Specific PCR (KASPar) assays have been developed in chickpea and pigeonpea. By using above resources, the first-generation or comprehensive genetic maps have been developed in the three legume speciesmentioned above. Analysis of phenotyping data together with genotyping data has provided candidate markers for drought-tolerance-related root traits in chickpea, resistance to foliar diseases in groundnut and sterility mosaic disease (SMD) and fertility restoration in pigeonpea. Together with these trait-associated markers along with those already available, molecular breeding programmes have been initiated for enhancing drought tolerance, resistance to

  6. Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans.

    Science.gov (United States)

    Suzuki, Osamu; Abe, Masafumi

    2014-05-01

    Galectin-1 is known to be one of the extracellular matrix proteins. To elucidate the biological roles of galectin-1 in cell adhesion and invasion of human anaplastic large cell lymphoma, we performed cell adhesion and invasion assays using the anaplastic large cell lymphoma cell line H-ALCL, which was previously established in our laboratory. From the cell surface lectin array, treatment with neuraminidase from Arthrobacter ureafaciens which cleaves all linkage types of cell surface sialic acid enhanced Arachis hypogaea (PNA), Helix pomatia (HPA) and Phaseolus vulgaris-L (L-PHA) lectin binding reactivity to cell surface of lymphoma cells suggesting that neuraminidase removes cell surface sialic acid. In cell adhesion and invasion assays treatment with neuraminidase markedly enhanced cell adhesion to galectin-1 and decreased cell invasive capacity through galectin-1. α2,6-linked sialic acid may be involved in masking the effect of the interaction between galectin-1 and cell surface glycans. H-ALCL cells expressed the β-galactoside-α2,6-sialyltransferase ST6Gal1. On resialylation assay by recombinant ST6Gal1 with CMP-Neu5Ac, α2,6-resialylation of L-PHA reactive oligosaccharide by ST6Gal1 resulted in inhibition of H-ALCL cell adhesion to galectin-1 compared to the desialylated H-ALCL cells. On knockdown experiments, knockdown of ST6Gal1 dramatically enhanced cell adhesion to galectin-1. N-glycosylation inhibitor swainsonine treatment resulted in enhancement of cell adhesion to galectin-1. In glycomic analysis using the lectin blocking assay treatment with PNA, Artocarpus integrifolia (Jacalin), Glycine max (SBA), Helix pomatia (HPA), Vicia villosa (VVA), Ulex europaeus (UEA-1), Triticum vulgaris (WGA), Canavalia ensiformis (ConA), Phaseolus vulgaris-L (L-PHA), Phaseolus vulgaris-E4 (E-PHA), Datura stramonium (DSA) lectins resulted in modulation of lymphoma cell to galectin-1 suggesting that several types of glycans may regulate cell adhesion to galectin-1 by

  7. Effects of Different Planting Patterns on Yield and Quality of Peanut in Northwest Hebei Province%不同种植方式对冀西北花生产量和品质的影响

    Institute of Scientific and Technical Information of China (English)

    王激清; 左利兵; 刘社平

    2011-01-01

    Ordinary peanut and color peanut were used as experiment material. Effects of three planting patterns, film covering and double ridge planting, single ridge planting and flat land drilling, on yield and quality of peanut were studied in northwest Hebei province. The results showed that comparing with single ridge planting and flat land drilling, film covering and double ridge planting could increase the germination rate of peanut and make the seedling stage, flowering stage and maturity stage earlier. Film covering and double ridge planting could significantly improve 100-pod weight and 100-seed weight and increase the number of pod per plant, so as to significantly increase yield of peanut. In addition, planting patterns affected quality of peanut. Film covering and double ridge planting could significantly increase protein content, ratio of oleic acid and linoleic acid. Results also showed that yield and protein content of color peanut were higher than those of ordinary peanut under same planting pattern. Therefore, color peanut has greater production potential in northwest Hebei province.%以普通花生(Arachis hypogaea L.)和引入的彩色花生为试验材料,研究覆膜双行垄种、单行垄种和平地直接条播3种种植方式对冀西北地区花生产量和品质的影响.结果表明,覆膜双行垄种能提高花生出苗率,且使花生出苗期、开花期和成熟期明显提前,与单行垄种和平地直接条播相比.覆膜双行垄种能显著提高花生的百果重和百仁重,并能增加花生的单株结果数,因此能显著提高花生产量;不同种植方式对花生子仁品质也有影响,覆膜双行垄种能显著提高花生蛋白质含量、油酸/亚油酸比值.同一种植方式下,彩色花生的产量和蛋白质含量高于普通花生,在冀西北地区有更大的推广应用潜力.

  8. Response of groundnut to deficit irrigation during vegetative growth

    International Nuclear Information System (INIS)

    Studies were conducted to investigate patterns of field water use efficiency (WUE) by groundnut under moisture stress. Experiment 1 examined four cultivars of Spanish groundnut (Arachis hypogaea L. ssp. fastigiata var. vulgaris), Ak 12-24, J 11, GAUG 1 and GG 2, during the summer seasons of 1989 and 1990. The crops were subjected to soil moisture deficit stress by withholding irrigation for 30 d, starting at 20 d after sowing (treatment T 101), and for 25 d starting at 20 d (T 102). In treatment T 101, the stress period was followed by two relief irrigations with an interval of 5 d. In Experiment 2, stress was imposed on cv. GG 2 from 10 to 30 d after sowing, based on an irrigation water/cumulative pan evaporation (IW/CPE) ratio of 0.6. In one case, this period of stress was followed with irrigation to maintain IW/CPE at 1.0 until harvest (T 200, control); in the second case, the initial stress period was followed by maintenance of IW/CPE at 0.8 until harvest (treatment T 201); in the third case, IW/CPE of 0.6 was continued until harvest (treatment T 202). The results of Experiment 1 showed that leaf area indices of plants stressed during the vegetative phase were higher during the reproductive phase, i.e. from 80 d after sowing until harvest, than those of control (T 100) plants. In general, percent dry matter distribution to leaves remained higher in control plants; percent dry matter distribution to the leaves of stressed plants was lower both during and after stress. Dry matter distributions to pods, and thus the harvest indices, were higher in the stressed plants. Among the treatments, total biomass and economic yields were higher in T 102, followed by T 101. The results of Experiment 2 were consistent with those of Experiment 1. Where groundnut is cultivated with irrigation, it is possible to increase field WUE and dry matter production, including economic yield, by imposing a transient deficit in soil moisture during the vegetative phase. (author)

  9. The Research Progress of the Peanut Skin%花生红衣研究进展

    Institute of Scientific and Technical Information of China (English)

    张慧文; 许海燕; 马超美

    2015-01-01

    Peanut is an important crop as food and oil, while its seed skin is also known as a tradi-tional Chinese medicine.This review compiled reports of procyanidins at home and abroad, introduced the chemical compositions, biological activities and the application prospect of peanut skin in detail. Peanut is an important plant in China, with the highest production over the world.But there are much peanut skin wasted in the processing of peanut-derived products.The main chemical compositions in peanut skin were procyanidins, which were classified according to the polymerization degree, with fig-ures to illustrate each compound.This paper summarized the current knowledge on the biological ac-tivities, such as antioxidant and antiallergic activity, inhibition on hyperlipidemia and diabetes, etc. Based on the latest studies at home and abroad, peanut skin is considered to be with great application in the field of food and medicine for its strong antioxidant activities and low toxicity.%花生是我国的优势种植作物,产量位居世界首位,但是缺少深度开发,花生红衣的利用率低下。花生红衣是重要粮油作物花生(Arachis hypogaea .L)的种皮,同时也是一味传统中药。花生红衣中的化学成分主要是原花青素,根据成分的聚合度进行分类。原花青素具有很强的生物活性,如抗氧化、降血脂、降血糖和抗过敏等,又具食源性和低毒性的特点,因此在食品和药品领域有巨大的应用前景和实用价值。本文对花生生产的基本情况、花生红衣的化学成分、生物活性和应用前景进行了详细综述。

  10. 花生红衣研究进展%The Research Progress of the Peanut Skin

    Institute of Scientific and Technical Information of China (English)

    张慧文; 许海燕; 马超美

    2015-01-01

    花生是我国的优势种植作物,产量位居世界首位,但是缺少深度开发,花生红衣的利用率低下。花生红衣是重要粮油作物花生(Arachis hypogaea .L)的种皮,同时也是一味传统中药。花生红衣中的化学成分主要是原花青素,根据成分的聚合度进行分类。原花青素具有很强的生物活性,如抗氧化、降血脂、降血糖和抗过敏等,又具食源性和低毒性的特点,因此在食品和药品领域有巨大的应用前景和实用价值。本文对花生生产的基本情况、花生红衣的化学成分、生物活性和应用前景进行了详细综述。%Peanut is an important crop as food and oil, while its seed skin is also known as a tradi-tional Chinese medicine.This review compiled reports of procyanidins at home and abroad, introduced the chemical compositions, biological activities and the application prospect of peanut skin in detail. Peanut is an important plant in China, with the highest production over the world.But there are much peanut skin wasted in the processing of peanut-derived products.The main chemical compositions in peanut skin were procyanidins, which were classified according to the polymerization degree, with fig-ures to illustrate each compound.This paper summarized the current knowledge on the biological ac-tivities, such as antioxidant and antiallergic activity, inhibition on hyperlipidemia and diabetes, etc. Based on the latest studies at home and abroad, peanut skin is considered to be with great application in the field of food and medicine for its strong antioxidant activities and low toxicity.

  11. Ensaios de variedades de amendoim: III - Décima e décima-primeira séries de ensaios Peanut variety trials: III

    Directory of Open Access Journals (Sweden)

    Vicente Canecchio Filho

    1957-01-01

    Full Text Available No presente trabalho são relatadas experiências com 16 variedades de amendoim (Arachis hypogaea, L., recebidas dos Estados Unidos da América do Norte, do Congo Belga e de várias regiões do Brasil. Essas experiências, em número de oito, das quais três pertencentes à décima série (ano agrícola de 1953/54 e cinco compreendendo a décima-primeira série (ano agrícola de 1954/55, foram executadas nas localidades de Campinas, Ribeirão Prêto, Pindorama, Presidente Prudente e Tatuí, no Estado de São Paulo. Os resultados obtidos mostraram que, para as condições em que foram realizados os ensaios, as variedades Paulista-269, Bandeirante-263, Brasília-265, Centenário-264 e Tatuí-76, sobressairam-se das demais, notadamente a primeira, que, de um modo geral, foi bem classificada em todas as localidades, não só em relação à produção como pelo alto teor em óleo, nos frutos. A variedade Tatu-53 (testemunha, ainda bastante cultivada no Estado, classificou-se entre as piores. As maiores produções foram obtidas em terra arenosa. Nas terras roxa e roxa-misturada, também se conseguiram boas produções. Já na região representada pela terra massapê as produções foram fracas, confirmando os resultados dos anos anteriores.This paper reports the results obtained from field tests with sexteen peanut varieties received from the United States of America, Belgium Congo and several Brazilian regions. Eight experiments were conducted in the following counties of the State of São Paulo: Campinas, Ribeirão Preto, Pindorama, Presidente Prudente and Tatuí. Yield data showed the varieties Paulista-269, Bandeirante-263, Brasilia-265, Centenário-264 and Tatui-76 to prove better, particularly the first one, that ranked good classification in every locality as far as seed production and oil content of the seeds are concerned. Variety Tatu-53 which still widespreads all over the State, was one of the least yielders. The highest yields were

  12. Produtividade da cana-de-açúcar após o cultivo de leguminosas

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    Edmilson José Ambrosano

    2011-01-01

    Full Text Available Estudou-se o efeito do cultivo prévio de leguminosas sobre a produtividade e lucratividade da cana-de-açúcar. Foram determinados a produtividade de biomassa, o acúmulo de nutrientes das leguminosas, a ocorrência natural de fungos micorrízicos arbusculares, bem como o efeito das leguminosas sobre a população de nematoides do gênero Pratylenchus à cana-de-açúcar. O experimento foi desenvolvido em Piracicaba (SP, Brasil, em solo classificado como Argissolo Vermelho-Amarelo distrófico, utilizando-se a cultivar de cana-de-açúcar (Saccharum spp. IAC87-3396. As avaliações dos efeitos do cultivo prévio das leguminosas foram realizadas durante cinco cortes consecutivos. Os tratamentos consistiram do cultivo prévio das leguminosas: Amendoim (Arachis hypogaea L - cultivares IAC-Tatu e IAC-Caiapó, crotalária júncea IAC 1 (Crotalaria juncea L e mucuna preta [Mucuna aterrima (Piper & Tracy Holland], e um tratamento-testemunha. Adotou-se o delineamento em blocos casualizados com cinco repetições. A leguminosa mais produtiva de biomassa seca (parte aérea+raízes foi a crotalária júncea IAC 1 (10.264 kg ha-1, seguida da mucuna preta (4.391 kg ha-1 e dos amendoins, IAC-Caiapó (3.177 kg ha-1 e IAC-Tatu (1.965 kg ha-1. O amendoim IAC-Caiapó e a mucuna preta foram as espécies mais infectadas por fungos micorrízicos. O amendoim, independente da cultivar, foi a leguminosa que mais reduziu a infestação de Pratylenchus spp. na cana-de-açúcar. Após cinco cortes da cana-de-açúcar o melhor desempenho foi notado no tratamento com cultivo prévio de crotalária júncea IAC 1, o qual promoveu incrementos de 30% e 35% na produtividade de colmos e de açúcar respectivamente e o melhor desempenho econômico.

  13. Detection, Characterization, and Biological Effect of Quorum-Sensing Signaling Molecules in Peanut-Nodulating Bradyrhizobia

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    Walter Giordano

    2012-03-01

    Full Text Available Bacteria of the genus Bradyrhizobium are able to establish a symbiotic relationship with peanut (Arachis hypogaea root cells and to fix atmospheric nitrogen by converting it to nitrogenous compounds. Quorum sensing (QS is a cell-cell communication mechanism employed by a variety of bacterial species to coordinate behavior at a community level through regulation of gene expression. The QS process depends on bacterial production of various signaling molecules, among which the N-acylhomoserine lactones (AHLs are most commonly used by Gram-negative bacteria. Some previous reports have shown the production of QS signaling molecules by various rhizobia, but little is known regarding mechanisms of communication among peanut-nodulating strains. The aims of this study were to identify and characterize QS signals produced by peanut-nodulating bradyrhizobial strains and to evaluate their effects on processes related to cell interaction. Detection of AHLs in 53 rhizobial strains was performed using the biosensor strains Agrobacterium tumefaciens NTL4 (pZLR4 and Chromobacterium violaceum CV026 for AHLs with long and short acyl chains, respectively. None of the strains screened were found to produce AHLs with short acyl chains, but 14 strains produced AHLs with long acyl chains. These 14 AHL-producing strains were further studied by quantification of β-galactosidase activity levels (AHL-like inducer activity in NTL4 (pZLR4. Strains displaying moderate to high levels of AHL-like inducer activity were subjected to chemical identification of signaling molecules by high-performance liquid chromatography coupled to mass spectrometry (LC-MS/MS. For each AHL-producing strain, we found at least four different AHLs, corresponding to N-hexanoyl-DL-homoserine lactone (C6, N-(3-oxodecanoyl-L-homoserine lactone (3OC10, N-(3-oxododecanoyl-L-homoserine lactone (3OC12, and N-(3-oxotetradecanoyl-L-homoserine lactone (3OC14. Biological roles of 3OC10, 3OC12, and 3OC14 AHLs

  14. Biocompatibility of sweetpotato and peanut in a hydroponic system

    Science.gov (United States)

    Mortley, D. G.; Loretan, P. A.; Hill, W. A.; Bonsi, C. K.; Morris, C. E.; Hall, R.; Sullen, D.

    1998-01-01

    'Georgia Red' peanut (Arachis hypogaea L.) and TU-82-155 sweetpotato [Ipomoea batatas (L.) Lam] were grown in monocultured or intercropped recirculating hydroponic systems in a greenhouse using the nutrient film technique (NFT). The objective was to determine whether growth and subsequent yield would be affected by intercropping. Treatments were sweetpotato monoculture (SP), peanut monoculture (PN), and sweetpotato and peanut grown in separate NFT channels but sharing a common nutrient solution (SP-PN). Greenhouse conditions ranged from 24 to 33 degrees C, 60% to 90% relative humidity (RH), and photosynthetic photon flux (PPF) of 200 to 1700 micromoles m-2 s-1. Sweetpotato cuttings (15 cm long) and 14-day-old seedlings of peanuts were planted into growth channels (0.15 x 0.15 x 1.2 m). Plants were spaced 25 cm apart within and 25 cm apart between growing channels. A modified half-Hoagland solution with a 1 N: 2.4 K ratio was used. Solution pH was maintained between 5.5 and 6.0 for treatments involving SP and 6.4 and 6.7 for PN. Electrical conductivity (EC) ranged between 1100 and 1200 microS cm-1. The number of storage roots per sweetpotato plant was similar for both SP and SP-PN. Storage root fresh and dry mass were 29% and 36% greater, respectively, for plants in the SP-PN treatment than for plants in the SP treatment. The percent dry mass of the storage roots, dry mass of fibrous and pencil roots, and the length-to-diameter ratio of storage roots were similar for SP and SP-PN sweetpotato plants. Likewise, foliage fresh and dry mass and harvest index were not significantly influenced by treatment. Total dry mass was 37% greater for PN than for SP-PN peanut plants, and pod dry mass was 82% higher. Mature and total seed dry mass and fibrous root dry mass were significantly greater for PN than for SP-PN plants. Harvest index (HI) was similar for both treatments. Root length tended to be lower for seedlings grown in the nutrient solution from the SP-PN treatment.

  15. 海南剑麻园杂草种类调查及防除技术研究%Survey of Weeds in Sisal Plantation and Control Techniques in Hainan

    Institute of Scientific and Technical Information of China (English)

    李晓霞; 沈奕德; 黄乔乔; 范志伟; 易克贤; 黄东东

    2014-01-01

    A systematical investigation of weeds was taken in sisal plantation in Hainan province , and the dominant weed groups were determined.Then, weed control experiments were conducted.The results showed that there were 121 weed species belonging to 21 families in the plantation.Among them, eight species were considered as dominant weeds , including Melinis repens, Panicum maximum, Imperata cylindrica, Digitaria setigera, Chromolaena odorata, Praxelis clematidea, Tridax procumbens and Mimosa pudica.Leguminous crops as intercrops , perennial vines as covering plants could control the weeds to some extent.Arachis hypogaea and Macroptilium atropurpureum as intercrops could have evident effect on controlling the weeds, and leguminous plants could increase the soil fertility.Therefore, their application could be further extended in this area.%本文系统调查了海南剑麻主要种植区的杂草种类,确定了剑麻园的杂草优势种群,并以此为基础进行了剑麻园除草试验。结果表明,本区剑麻园杂草发生种类共有21科121种,其中优势种杂草主要为红毛草、大黍、白茅、短颖马唐、飞机草、假臭草、羽芒菊和含羞草8种杂草;采用间种豆科植物或多年生藤本植物覆盖控草可在一定程度上控制杂草的发生,其中间种花生、大翼豆处理具有明显的防除效果,且间种豆科植物能提高土壤肥力,可以在该地区进一步推广应用。

  16. Crop rotation biomass and arbuscular mycorrhizal fungi effects on sugarcane yield Produção de biomassa e presença de fungos micorrízicos arbusculares em culturas utilizadas em rotação com a cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Edmilson José Ambrosano

    2010-12-01

    Full Text Available A cana-de-açúcar (Saccharum spp. vem sendo cultivada no Brasil para produção de açúcar e agroenergia. Em seu sistema de produção, após um ciclo de 4 a 8 anos, é possível a rotação com plantas de cobertura, antes do seu replantio, para melhoria do solo e geração de renda. Estudou-se a caracterização e produtividade de biomassa de leguminosas (como adubos-verdes e girassol (Helianthus annuus L., a ocorrência natural de micorrizas, o teor de açúcar e a produtividade em colmos da cana-de-açúcar IAC 87-3396 e a viabilidade econômica desse sistema com cultivo após as opções de rotação, com quantificação da produtividade durante três cortes consecutivos. O amendoim (Arachis hypogaea L. cv. IAC-Caiapó, girassol cv. IAC-Uruguai e mucuna-preta (Mucuna aterrimum Piper and Tracy foram as culturas que apresentaram maior percentagem de colonização por fungos micorrízicos. O girassol foi a planta de cobertura que mais extraiu nutrientes do solo, seguido por amendoim (Arachis hipogaea L. cv. IAC-Tatu e feijão-mungo (Vigna radiata L. Wilczek. A colonização por fungos micorrízicos mostrou correlação positiva com a altura de plantas de cana no primeiro corte (p = 0,01 e R = 0,52, mas não se correlacionou com a produtividade de colmos ou açúcar. No primeiro corte, o girassol foi a cultura de rotação que ocasionou o maior aumento de produtividade, da ordem de 46% em colmos e de 50% na quantidade de açúcar, em comparação com a testemunha. Com exceção dos amendoins, todas as culturas em rotação aumentaram a renda líquida do sistema na média de três cortes de cana-de-açúcar.Sugarcane (Saccharum spp. is an important crop for sugar production and agro-energy purposes in Brazil. In the sugarcane production system after a 4- to 8-year cycle crop rotation may be used before replanting sugarcane to improve soil conditions and give an extra income. This study had the objective of characterizing the biomass and the

  17. A good forage and cover plant, Arachis pintoi cv. Reyan No.12, its selecting, breeding, extension and utilization%热研12号平托落花生选育及推广利用

    Institute of Scientific and Technical Information of China (English)

    白昌军; 刘国道

    2006-01-01

    通过对热研12号平托落花生Ayachis pintoi cv.Reyan No. 12的一系列试验研究,结果表明:热研12号平托落花生具发达的匍匐茎,可在短期内形成20 cm高的致密草层,具有较高的饲草产量和营养价值,同时花期长达10个月以上,开花时满地金黄,坪面整齐,具有良好的园林绿化效果,综合性状优于早年引种的平托落花生品种Amarillo,是牧草和园林植物兼用型落花生属新品种,已在海南、广东、广西等省区推广种植,适合我国华南、东南及西南地区作为饲用牧草和园林绿化兼用型植物推广.

  18. How do cowpea and groundnut improve soil N fertility and yields of succeeding sorghum crop in the Guinean savannah zone of Burkina Faso (West Africa)?

    International Nuclear Information System (INIS)

    Field experiments were carried out in a weakly acid (pH H2O 6.5) Ultisol at Farako-Ba (4 deg 20' West, 11 deg 6' North and 405 m altitude) in the Guinean savannah zone of Burkina Faso to study the beneficial effects of cowpea (Vigna unguiculata) and groundnut (Arachis hypogaea) on grain yield of succeeding sorghum. The first experiment was a factorial of three crop rotations (cowpea-sorghum, groundnut-sorghum and sorghum-sorghum) as first factor and five fertilizer treatments (NPK fertilizer, NPK+ dolomite, NPK+ manure, NK+ phosphate rock and a control) as second factor in a split plot design with a randomised block arrangement and four replications. Soil mineral N, nematode infection and fertilizer N recoveries were studied during three years (2000 to 2002). Biological nitrogen fixation (BNF) in cowpea and groundnut was measured in the second year (2001) with 15N isotopic dilution method using a non-fixing cowpea as reference crop. Groundnut fixed 8 to 23 kg N ha-1 and the percent of N derived from the atmosphere (% Ndfa) varied from 27 to 34%. Cowpea fixed 50 to 115 kg N ha-1 and the Ndfa varied from 52 to 56%. Compared to mineral NPK fertilizer alone, legumes fixed more nitrogen from the atmosphere when NPK fertilizer was combined with dolomite or manure. Compared to water-soluble phosphate, phosphate rock increased BNF by cowpea. Highest responses to N fertilizer applications were found when sorghum was rotated with legumes and the lowest in mono cropping of cereals. Compared to mono cropping of cereals (maize-sorghum or sorghum-sorghum), sorghum produced 2.9 and 3.1 times more grain yields when it was rotated with groundnut or cowpea respectively. In a second experiment, the response of a succeeding sorghum to N fertilizer, nitrogen fertilizer equivalencies (NFE) of the two legumes and nematode infection in the soil and roots of the succeeding sorghum were studied. The NFE of groundnut (35 kg N ha-1) was higher than that of cowpea (25 kg N ha-1). However, soil

  19. Inoculação com Rhizobium e aplicação de nitrogênio em amendoim Comparison among Rhizobium strains inoculations and nitrogen applications on peanut, in field conditions

    Directory of Open Access Journals (Sweden)

    Antonio Roberto Giardini

    1985-01-01

    Full Text Available Existe, nas nossas condições, uma população autóctone de Rhizobium capaz de nodular o amendoim (Arachis hypogaea L., mas pouco se sabe da contribuição do nitrogênio fixado para esta planta. Foram conduzidos dois ensaios no campo, em solo de baixa fertilidade, um no período "da seca" e outro no "das águas", comparando o crescimento e a produção de plantas de amendoim inoculado com Rhizobium selecionado, com o de plantas noduladas pela população autóctone, adubadas ou não com nitrogênio. A nodulação das plantas inoculadas foi semelhante à observada nos tratamentos não inoculados, com ou sem nitrogênio. Na fase final do ciclo das plantas, houve maior acúmulo e maior taxa de absorção diária de nitrogênio nos tratamentos inoculados ou com adubação nitrogenada, do que no controle sem inoculação e sem nitrogênio. No ensaio da seca, não houve aumento de produção devido à adubação nitrogenada, ou à inoculação. No ensaio das águas, houve resposta à aplicação de nitrogênio no plantio. Os resultados de produção não foram coerentes com os da marcha de absorção de N. A produção de ensaio das águas foi equivalente a 3.400 kg/ha para o tratamento sem nitrogênio e sem inoculação.Two field experiments were carried out with peanut in the same area on a limed and fertilized "cerrado soil" (originally acidic and low fertility. The first experiment was carried out in the autumn/winter (dry season, and the second one in the subsequent spring/summer (wet season, in Campinas, State of São Paulo, Brazil. Plant development and production of inoculated (three Rhizobium strains and nitrogen fertilized treatments (at planting 25 and 45 days after planting were compared with non-inoculated and non-N-fertilized control. Nodulation of inoculated plants was similar to those of non-inoculated, with or without nitrogen. Greater accumulations, and rates for average daily uptake of nitrogen were observed for inoculated as

  20. Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticus infection

    Directory of Open Access Journals (Sweden)

    Liang Xuanqiang

    2008-02-01

    Full Text Available Abstract Background Peanut (Arachis hypogaea L. is an important crop economically and nutritionally, and is one of the most susceptible host crops to colonization of Aspergillus parasiticus and subsequent aflatoxin contamination. Knowledge from molecular genetic studies could help to devise strategies in alleviating this problem; however, few peanut DNA sequences are available in the public database. In order to understand the molecular basis of host resistance to aflatoxin contamination, a large-scale project was conducted to generate expressed sequence tags (ESTs from developing seeds to identify resistance-related genes involved in defense response against Aspergillus infection and subsequent aflatoxin contamination. Results We constructed six different cDNA libraries derived from developing peanut seeds at three reproduction stages (R5, R6 and R7 from a resistant and a susceptible cultivated peanut genotypes, 'Tifrunner' (susceptible to Aspergillus infection with higher aflatoxin contamination and resistant to TSWV and 'GT-C20' (resistant to Aspergillus with reduced aflatoxin contamination and susceptible to TSWV. The developing peanut seed tissues were challenged by A. parasiticus and drought stress in the field. A total of 24,192 randomly selected cDNA clones from six libraries were sequenced. After removing vector sequences and quality trimming, 21,777 high-quality EST sequences were generated. Sequence clustering and assembling resulted in 8,689 unique EST sequences with 1,741 tentative consensus EST sequences (TCs and 6,948 singleton ESTs. Functional classification was performed according to MIPS functional catalogue criteria. The unique EST sequences were divided into twenty-two categories. A similarity search against the non-redundant protein database available from NCBI indicated that 84.78% of total ESTs showed significant similarity to known proteins, of which 165 genes had been previously reported in peanuts. There were

  1. The use of legume trap crops for control of Striga hermonthica (Del.) Benth. in sorghum (Sorghum bicolor L. Moench) in northern Nigeria.

    Science.gov (United States)

    Gworgwor, Nuhu A

    2002-01-01

    Two different field trials (one trial on sorghum intercropped with groundnut [Arachis hypogaea L.], and another trial on sorghum intercropped with bambara groundnut [Vigna subterranea L. Verdc.]) were conducted during the 1995 and 1996 rainy seasons at the Teaching and Research Farm of the Department of Crop Science, Faculty of Agriculture, University of Maiduguri, Maiduguri (11 degrees 51' N; 13 degrees 15' E) to evaluate the effect of intercropping resistant and susceptible sorghum varieties with the two legume crops for the control of Striga hermonthica (Del.) Benth, in sorghum. The sorghum varieties used for both trials were ICSV 1002, ICSV 1007 (resistant varieties) and War-warabashi (susceptible). Ex-Dakar variety of groundnut was used for the sorghum-groundnut trial, while a creamed brown eyed local bambara groundnut was used for the sorghum-bambara groundnut trial. There were six treatments with the groundnut trial and nine treatments with the bambara trial and all were laid out in a randomised complete block design (RCBD) replicated four times. In the sorghum-groundnut trial, the results show that the intercropping of sorghum with groundnut significantly reduced Striga infestation up to 50% in sorghum in both years. Both resistant varieties supported significantly fewer number of Striga on sorghum when intercropped with groundnut compared with the sole sown susceptible variety. In both years and the combined analyses of 1995 and 1996 data, however, there was no significant difference in grain yield of sorghum due to the treatments. In the sorghum-bambara groundnut trial, the results show that Striga shoot count at harvest in sole sown ICSV 1007 was significantly lower than the ICSV 1002 variety, while the susceptible variety supported significantly higher Striga shoot count than the resistant varieties in both years and the combined analyses. Alternating stands of sorghum and bambara groundnut within the same row, in general, reduced Striga shoot count in

  2. Características agronômicas e produtividade de amendoim em diferentes espaçamentos e épocas de semeadura no Recôncavo Baiano Agronomic characteristics and yield of peanut under different sowing dates and planting arrangements in the Reconcavo Region of Bahia State

    Directory of Open Access Journals (Sweden)

    Clovis Pereira Peixoto

    2008-01-01

    Full Text Available Avaliou-se o desempenho vegetativo e produtivo de plantas de amendoim (Arachis hypogaea L. sob diferentes espaçamentos e densidades e em duas épocas de semeadura, tendo como primeira época o início da estação chuvosa (março-junho e como segunda, o fim do período (julho-outubro nas condições agroclimáticas do Recôncavo Baiano. O trabalho foi desenvolvido no campo experimental do Centro de Ciências Agrárias e Biológicas da Universidade Federal do Recôncavo da Bahia, no delineamento de blocos casualizados em esquema fatorial 3 x 3 + 1; três densidades de plantas: 5, 10, e 15 plantas m-1, três espaçamentos: 0,50 m, 0,65 m e 0,80 m entrelinhas, com um tratamento testemunha em covas espaçadas de aproximadamente 0,25 m x 0,30 m, com quatro repetições. As características agronômicas: altura final de plantas, número de ramificações finais e número de folhas foram determinados em 10 plantas da parcela útil, enquanto o volume de vagens frescas e a produtividade de vagens e grãos foram determinados com base na população de plantas. Conclui-se que a escolha do arranjo espacial que expressa maior produtividade de vagens e de grãos depende do objetivo comercial do produtor (volume ou massa e da época de semeadura. A utilização de novos arranjos espaciais pelos agricultores do Recôncavo Baiano, se faz necessário, uma vez que a semeadura em covas (0,25 m x 0,30 m mostra-se menos produtiva nas duas épocas.The vegetative and productive performance of peanut plants, cultivated under different spacing and planting densities, at two sowings dates, in the raining season (march-june and by July-October were evaluated in the agro ecologic conditions of the Recôncavo Region of the state of Bahia, Brazil. The experiment was carried out at the Experimental Station of the School of Agronomy, Federal University of Recôncavo of Bahia, in a randomized complete block design, with the following factorial arrangement: 3 x 3 + 1 (three

  3. Growth characteristics of multipurpose tree species,crop productivity and soil properties in agroforestry systems under subtropical humid climate in India

    Institute of Scientific and Technical Information of China (English)

    M. Datta; N. P. Singh

    2007-01-01

    Multipurpose tree species (MPTs) were studied in an agroforestry arboretum under subtropical humid climate in Northeast India. Out of 12 MPTs planted under agroforestry systems, Acacia auriculiformis in spacing of 2 m × 2 m (2500 stems·hm-2) could have the potentiality to meet the timber/fuelwood requirement due to its high wood production of 635 m3·hm-2 with mean annual increment (MAI) of 2.54×10-2· m3·tree1·a-1 in a short rotation period of 10 years. Thus, A. Auriculiformis is a short rotation forest tree species suitable to grow in subtropical humid climate. On the other hand, at 16 years of age, Eucalyptus hybrid and Michelia champaca in spacing of 3 m × 3 m (1111 stems·hm-2) produced appreciably high timber volume of 315 m3·hm-2 and 165 m3·hm-2 with MAI of 1.77×10-2 m3·tree-1·a-1 and 0.92×10-2 m3·tree-1·a-1, respectively. At 16 years of age, Gmelina arborea produced a timber volume of 147 m3·hm-2 with MAI of 1.47×10-2 m3·tree-1·a-1 followed by Samania saman (140 m3·hm-2), Albizzia procera (113 m3·hm-2) and Tectona grandis (79 m3·hm-2) with MAI of 1.40, 1.13 and 0.78 × 10-2 m3 ·tree-1·a-1, respectively in 4 m × 4 m spacing (625 stems·hm-2). Gliricidia maculata and Leucaena leucocephala could be used as live fences around the farm boundary to supply their N-rich leaves for mulch as well as manure to crops. In agroforestry arboretum, direct seeded upland rice (Oryza sativa - variety, AR-11), groundnut (Arachis hypogaea - variety, JL-24) and sesamum (Sesamum indicum - variety, B-67) were grown during the initial period upto 8 years of tree establishment. Under other MPTs,there was a reduction in crop productivity as compared to open space. After 8 years of tree establishment, horti-silvi and silvi-pastoral systems were developed and pineapple (Ananas comosus - variety Queen), turmeric (Curcuma longa -variety RCT -1) and cowpea (Vigna sinensis - variety Pusa Barsati) as forage crop were raised. The productivity of pineapple, turmeric

  4. Formulation and evaluation of indomethacin biomicroparticles

    Directory of Open Access Journals (Sweden)

    Sudeep Gupta

    2012-01-01

    Full Text Available Indomethacin microparticles prepared by extraction of bio polymer from arachis hypogen. in vitro drug release study was carried out through egg shell membrane for 3 hrs and analyzed sample by UV spectroscopy at 320 nm. A novel bio membrane from arachis hypogen was isolated by simplify economical process. The % yield was found to be 80% and particle range was 32-65 μm.

  5. Formulation and evaluation of indomethacin biomicroparticles.

    Science.gov (United States)

    Gupta, Sudeep; Gaur, Pawan; Soam, Kulwant; Suyal, Ruchi; Rana, Vinod; Dabral, Prashant

    2012-03-01

    Indomethacin microparticles prepared by extraction of bio polymer from arachis hypogen. in vitro drug release study was carried out through egg shell membrane for 3 hrs and analyzed sample by UV spectroscopy at 320 nm. A novel bio membrane from arachis hypogen was isolated by simplify economical process. The % yield was found to be 80% and particle range was 32-65 μm. PMID:23066194

  6. Formulation and evaluation of indomethacin biomicroparticles

    OpenAIRE

    Sudeep Gupta; Pawan Gaur; Kulwant Soam; Ruchi Suyal; Vinod Rana; Prashant Dabral

    2012-01-01

    Indomethacin microparticles prepared by extraction of bio polymer from arachis hypogen. in vitro drug release study was carried out through egg shell membrane for 3 hrs and analyzed sample by UV spectroscopy at 320 nm. A novel bio membrane from arachis hypogen was isolated by simplify economical process. The % yield was found to be 80% and particle range was 32-65 μm.

  7. 不同季节及遮阴条件下多年生花生和郁南假花生的营养成分的研究%STUDY ON THE GROWTH AND CHANGE OF NUTRITIVE COMPOSITION OF ARACHIS PINTOI AND DESMODIUM HETEROCARPUM IN VARYING ECOLOGICAL CONDITION

    Institute of Scientific and Technical Information of China (English)

    罗刚跃; 陈兆平; 程双奇

    2000-01-01

    不同季节多年生花生和郁南假花生营养成分分析表明,多年生花生一年四季均可以利用,尤以夏秋季为最佳;郁南假花生冬季叶少、粗纤维含量高不适宜利用.另外,遮荫条件(80%、40%自然光)下郁南假花生维生素含量降低,适当地加强光照可能会提高此牧草的维生素的含量,从而提高营养价值.

  8. In situ degradability and selected ruminal constituents of sheep fed with peanut forage hay.

    Science.gov (United States)

    Fernandes, Gisele Machado; Possenti, Rosana Aparecida; Teixeira de Mattos, Waldssimiler; Schammass, Eliana Aparecida; Junior, Evaldo Ferrari

    2013-01-01

    Because legumes are a very important feed source for ruminants, the aim of this study was to evaluate the ideal inclusion level of hay Arachis pintoi cv. Belmonte in sheep diets by measuring the dry matter intake (DMI), concentration of volatile fatty acids, ammonia-nitrogen concentration, ruminal pH and the in situ degradability of dry matter (DM) and crude protein (CP). In the experiment with four sheep, a 4 × 4 Latin Square design was used with four periods and four treatments (0%, 30%, 60% and 100% Arachis replacing grass hay). Significant interactions were observed between treatments and sampling times for ammonia-nitrogen and acetate, propionate and butyrate concentration and the acetate:propionate ratio. The ruminal pH and total volatile fatty acids concentration were not affected by interaction between treatments and sampling time. The degradation of DM and CP was similar, rising with the increasing content of Arachis, showing a linear effect. The treatment containing 60% of Arachis showed best results, with good levels of daily weight gain and higher ruminal concentrations of volatile fatty acids. The legume showed high levels of CP, high digestibility and appropriate levels of fibre, with excellent standards of degradation and ruminal characteristics. The use of the legume  Arachis for ruminants is a promising option of nutrient supply to meet production demands of these animals. PMID:24016145

  9. The induction of rat bladder cancer by 2-naphthylamine

    OpenAIRE

    Hicks, R. M.; Wright, R.; Wakefield, J. St. J.

    1982-01-01

    The widely held belief that 2-naphthylamine is not carcinogenic for the rat has been re-examined. Twenty female Wistar rats were dosed by gastric intubation weekly for 57 weeks with 2-naphthylamine, 300 mg/kg body wt, in arachis oil and 20 controls were given arachis oil alone. Animals which became moribund were killed during the course of the experiment and the remainder after 100 weeks. A 2-naphthylamine-treated animal died at 21 weeks; all others survived 57 weeks or longer. The urinary tr...

  10. Pig Manure Maximum Loading Capacity of Red Clay Soil Under Peanut-Radish Rotation System in Southern China%花生-萝卜轮作体系下红黏土红壤对猪粪安全消纳能力研究

    Institute of Scientific and Technical Information of China (English)

    张迪; 周志高; 杨奕如; 王兴祥

    2014-01-01

    为减少养猪场废弃物排放和化肥使用,降低环境污染,最大限度将猪粪肥料化,采用盆栽试验方法,在自然降雨条件下模拟研究了亚热带红壤丘陵区花生-萝卜轮作体系下红黏土红壤对猪粪的安全消纳能力。试验设在江西鹰潭中国科学院红壤生态试验站,供试土壤肥力水平中等(有机碳5.16 g·kg-1,Olsen-P 28.34 mg·kg-1)。试验以3组化肥水平为基础(不施化肥,常规化肥减半,常规化肥用量),常规化肥年施肥量为:N 100 kg·hm-2,P 50 kg·hm-2,K 100 kg·hm-2。每个化肥用量基础上分别设置7个猪粪施肥梯度(以P计):0、25、50、100、200、400、800 kg P·hm-2。通过监测两年土壤渗漏水中铵态氮、硝态氮和总磷浓度变化,结合作物产量和土壤养分含量变化,初步确定了花生-萝卜轮作体系下红黏土红壤对猪粪的安全消纳量。结果表明:(1)从地下水和土壤环境角度分析,在不施化肥情况下,猪粪施用量应低于200 kg P·hm-2,常用化肥用量减半时,猪粪安全用量为不高于100 kg P·hm-2;常规化肥用量下,即使不施猪粪,渗漏水硝态氮浓度已经有引起污染的风险。(2)从作物产量角度考虑,猪粪和化肥配合施用时猪粪用量超过100 kg P·hm-2时不能使作物显著增产。因此,在保证经济效益和环境生态效益的同时,确定年化肥用量为常规用量减半水平,花生-萝卜轮作体系下红黏土红壤猪粪最大安全消纳量为100 kg P·hm-2,相当于每年施用鲜猪粪24000 kg·hm-2。%Studying pig manure maximum loading capacity of soil would maximize manure utilization, reduce use of chemical fertilizer and prevent environmental pollution. In this study, maximum loading capacity of red clay soil for pig manure in peanut(Arachis hypogaea L.)-radish(Raphanus sativus L.)system was investigated in a pot experiment under natural rainfall in

  11. Relative bioavailability of three newly developed albendazole formulations : a randomized crossover study with healthy volunteers

    NARCIS (Netherlands)

    Rigter, I M; Schipper, H G; Koopmans, R P; van Kan, H J M; Frijlink, H W; Kager, P A; Guchelaar, H-J

    2004-01-01

    This study of healthy volunteers shows that the relative bioavailability of albendazole formulations that use arachis oil-polysorbate 80 or hydroxypropyl-beta-cyclodextrin as an excipient was enhanced 4.3- and 9.7-fold compared to the results seen with commercial tablets. Administration of macrogol

  12. Establishment of five cover crops and total soil nutrient extraction in a humid tropical soil in the Peruvian Amazon

    Science.gov (United States)

    In order to evaluate the establishment of five cover crops and their potential to increase soil fertility through nutrient extraction, an experiment was installed in the Research Station of Choclino, San Martin, Peru. Five cover crops were planted: Arachis pintoi Krapov. & W.C. Greg, Calopogonium m...

  13. Dicty_cDB: Contig-U15976-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3933 ) AS1RN5P1H04.ab1 Healthy Roots (RN) Arachis stenos... 46 2.6 1 ( EE123931 )...-236P20, SP6 e... 46 2.6 1 ( BH906195 ) SALK_109427.51.10.x Arabidopsis thaliana TDNA ins... 46 2.6 1 ( EH04

  14. Dicty_cDB: Contig-U13744-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available -368K8 conta... 46 1.5 1 ( AJ859987 ) Brassica rapa subsp. pekinensis GSS, clone KBrH06... 46 1.5 1 ( EH043804 ) AS1RN40P1E05.ab1 Hea...lthy Roots (RN) Arachis steno... 46 1.5 1 ( AL138724 ) Human DNA sequence from clon

  15. Dicty_cDB: Contig-U01015-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3535 ) AS1RN35P1G02.ab1 Healthy Roots (RN) Arachis steno... 42 3.4 2 ( EJ542124 ) 1092955392044 Global-Ocean..._GS-36-01-01-2... 36 3.2 2 ( CW029681 ) 104_257_10499813_114_30401 Sorghum methylation fi... 44 3.3 2 ( EH04

  16. Comparison of peanut gentics and physical maps provided insights on collinearity, reversions and translocations

    Science.gov (United States)

    Genetic and physical maps are the valuable resources for peanut research community in understanding genome organization and serving as the basis for map-based cloning and marker-assisted selection. Physical maps of two diploid wild peanut progenitor species, Arachis duranensis (A genome) and A. ipae...

  17. Relative Bioavailability of Three Newly Developed Albendazole Formulations: a Randomized Crossover Study with Healthy Volunteers

    OpenAIRE

    Rigter, I. M.; Schipper, H.G.; Koopmans, R.P.; van Kan, H J M; Frijlink, H. W.; Kager, P A; Guchelaar, H.-J.

    2004-01-01

    This study of healthy volunteers shows that the relative bioavailability of albendazole formulations that use arachis oil-polysorbate 80 or hydroxypropyl-β-cyclodextrin as an excipient was enhanced 4.3- and 9.7-fold compared to the results seen with commercial tablets. Administration of macrogol suppositories did not result in measurable plasma concentrations of albendazole sulfoxide.

  18. Effect of broiler litter ash and flue gas desulfurization gypsum on yield, calcium and phosphorus uptake by peanut

    Science.gov (United States)

    Peanut (Arachis hyogaea) is an important oil seed crop that is grown as a principle source of edible oil and vegetable protein. Over 1.6 million acres of peanuts were planted in the United States during 2012. Peanuts require large amounts of Calcium (Ca) and Phosphorus (P). In 2010, over 10 milli...

  19. Effect of broiler litter ash and flue gas desulfurization gypsum on yield, calcium, phosphorus, copper, iron, manganese and zinc uptake by peanut

    Science.gov (United States)

    Peanut (Arachis hyogaea) is an important oil seed crop that is grown as a principle source of edible oil and vegetable protein. Over 1.6 million acres of peanuts were planted in the United States during 2012. Peanuts require large amounts of calcium (Ca) and phosphorus (P). In 2010, over 10 milli...

  20. A Steatite "Owl" Effigy from San Diego County, California

    OpenAIRE

    Musser, Ruth

    1980-01-01

    In the winter 1978 issue of the Journal of California Anthropology, Hudson (1978:262- 265) discussed an unusual stone effigy from San Clemente Island, which after a comparison with other effigies from that island he concluded to be a Burrowing Owl (Athene cunicularia hypogaea). Concurrently, as this report appeared in print, the subject of a special exhibit on display at Riverside Municipal Museum in Riverside, California, was an incised, mottled red steatite tablet. Incised lines on the tabl...

  1. Construction and Analysis of a Full-Length cDNA Library of Peanut Embryos at Different Developmental Stages%不同发育时期花生胚混合全长cDNA文库的构建与分析

    Institute of Scientific and Technical Information of China (English)

    陈华; 邓烨; 张冲; 蔡铁城; 郑奕雄; 庄伟建

    2014-01-01

    以及DREB转录因子等。%[Objective] The objective of this study is to understand the molecular mechanism of peanut embryo development and obtain important genes related to peanut embryo development. [Method] Using peanut variety Minhua 6 as the experimental material, embryos on 10, 20, 30, 40, 50, and 60th day after pegging were sampled. Total RNA was extracted by improved CTAB method. Double strand cDNA was synthesized based on SMART technique. The purified dscDNA was ligated to pDNR-LIB vector digested by SfiⅠ and transformed into DH5α by electroporation to construct a full-length cDNA library of peanut embryos at different developmental stages. Bioinformatics analysis was performed following small-scale EST sequencing.[Result]A successful full-length cDNA library of peanut embryos at different development stages was constructed. The titer of unamplified cDNA library was about 3.5×106cfu/mL. The average cDNA inserts were more than 1 000 bp with a recombination frequency of 95.8%. Small-scale plasmid extraction and subsequent sequencing resulted in 60 ESTs, which were used for further analysis. BLASTX analysis showed that 39 sequences (65% of total sequences) had high similarity with reported genes in Glycine max, Arachis hypogaea, Medicago truncatula, etc. on NCBI with 32 sequences having known or putative functions and functions of other 7 sequences were unclear. The other 21 (35%of total sequences) could not find similarity with known genes in NCBI, which may be novel genes for peanut. GO annotation was performed with BLAST2GO software and the results revealed that the ESTs generated in this study mainly included responsive to stresses and defenses, protein synthesis and transport, lipid synthesis and metabolism, transcription and regulation, seed germination, dormancy and embryo development related genes. Besides, some genes were involved in signal transduction and light morphogenesis process. KEGG pathway analysis showed that the ESTs generated by randomly sequencing in this study mainly

  2. Removal of Reactive Dyes (Green, Orange, and Yellow from Aqueous Solutions by Peanut Shell Powder as a Natural Adsorbent

    Directory of Open Access Journals (Sweden)

    Hosein Nadi

    2012-11-01

    . Adsorption of phenol and its derivative from water using synthetic resins and low- cost natural adsorbents: A review. J Environ manage 2009;90(3:1336–49. 23. Yang C, KE L, Gong R, Liu H, Sun Y. [Utilization of powdered peanut hull as biosorbent for removal of azo dyes from aqueous solution]. J Biol 2005;2:16. (Full Text in Chinese 24. Rasekh H, Safarzadeh Vishkaei MN, Asghari J. [Response of yield and qualitative characteristics of peanut (Arachis hypogaea L. to planting pattern and plant density in Guilan province]. J Agric Sci 2006;12(2:387-96. (Full Text in Persian 25. Shokoohi R, Vatanpoor V, Zarrabi M, Vatani A. Adsorption of Acid Red 18 (AR18 by Activated Carbon from Poplar Wood - A Kinetic and Equilibrium Study. E J Chem 2010;7(1:65-72. 26. Nagda GK, Diwan AM, Ghole VS. Potential of Tendu leaf refuse for phenol removal in aqueous systems. Appl Ecol Environ Res 2007;5(2:1-9. 27. Rahman IA, Saad B. Utilization of Guava seed as a source of activated carbon for removal of methylene blue from aqueous solution. Malays J Chem 2003;5(1:8-14. 28. Rafatullah M, Sulaiman O, Hashim R, Ahmad A. Adsorption of copper(II, chromium(III, nickel(II and lead(II ions from aqueous solutions by meranti sawdust. J Hazard Mater 2009;170(2-3:969–77. 29. Tanyildizi MŞ, Modeling of adsorption isotherms and kinetics of reactive dye from aqueous solution by peanut hull. Chem Eng J 2011;168(3:1234-40.

  3. 果园套种平托花生技术

    Institute of Scientific and Technical Information of China (English)

    郑向丽; 林永生; 郑仲登; 应朝阳; 黄毅斌

    2005-01-01

    平托花生(Arachis pintoi)亦称野花生、遍地黄金,是原产巴西的豆科牧草,在全球的热带、亚热带地区均有种植,主要用于人工草地、生物覆盖和观赏草坪。1989年通过中国-ACIAR合作项目首次从澳大利亚引入我国,福建省农科院经10多年的研究驯化,筛选出一个新型栽培种(Arachis pintoi cv.Amarillo),于2003年通过全国牧草品种审定委员会的审定,定名为“阿玛瑞罗平托花生”。

  4. Improved growth and nutrient status of an oat cover crop in sod-based versus conventional peanut-cotton rotations

    OpenAIRE

    Zhao, Duli; Wright, David L.; Marois, James J.; Mackowiak, Cheryl L; Brennan, Meghan

    2010-01-01

    International audience Nitrogen (N) leaching from agricultural soils is a major concern in the southeastern USA. A winter cover crop following the summer crop rotation is essential for controlling N leaching and soil run-off, thereby improving sustainable development. Rotation of peanut (Arachis hypogea L.) and cotton (Gossypium hirsutum L.) with bahiagrass (Paspalum notatum) (i.e. sod-based rotation) can greatly improve soil health and increase crop yields and profitability. In the sod-ba...

  5. Digestão dos carboidratos de alimentos volumosos em eqüinos Digestion of carbohydrates of forages in horses

    OpenAIRE

    Eliane da Silva Morgado; Fernando Queiroz de Almeida; Vinícius Pimentel Silva; Augusto Vidal da Costa Gomes; Leandro Galzerano; Henrique Torres Ventura; Liziana Maria Rodrigues

    2009-01-01

    Neste estudo, foram realizados dois ensaios com os objetivos de avaliar o fracionamento dos carboidratos de alimentos volumosos e estimar a digestibilidade desses nutrientes em dois ensaios de digestão com eqüinos. No ensaio 1, foram utilizados cinco eqüinos em delineamento de blocos casualizados, com sete tratamentos - fenos de alfafa (Medicago sativa); amendoim forrageiro (Arachis pintoi); desmódio (Desmodium ovalifolium); guandu (Cajanus cajan); macrotiloma (Macrotyloma axillare); estilosa...

  6. Dicty_cDB: Contig-U15425-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available zed libr... 46 1.8 1 ( EX326935 ) GQ02826.B7_C09 GQ028 - Cambium / phloem scrappi...ng... 46 1.8 1 ( EX317135 ) GQ02811.B7_G13 GQ028 - Cambium / phloem scrapping... 46 1.8 1 ( EX317035 ) GQ028...11.B7_C08 GQ028 - Cambium / phloem scrapping... 46 1.8 1 ( DX511975 ) 762_1_14169231_5489_43582_012 Arachis

  7. VEGETABLE OILS FOR HIGH VOLTAGE INSULATION BY

    OpenAIRE

    Ezechukwu O. A

    2013-01-01

    This paper discusses the findings of the study of the possibility of using some vegetable oils as high voltage insulation materials. Samples of palm oil (elaeis  guineensis), palm kernel oil (elaeis  guineensis), coconut oil (cocos nucifera), groundnut oil(Arachis hypogea) and local pear oil(dacryodes  edulis) were obtained from Ekwulobia town, processed and tested. The results obtained showed exciting performance for palm oil, palm kernel oil and groundnut oil. Investigation on the dielectri...

  8. Soil organic matter in the granulometric fractions of an oxissol under different uses and management systems/
    Matéria orgânica nas frações granulométricas de um latossolo vermelho distroférrico sob diferentes sistemas de uso e manejo

    OpenAIRE

    Marie-Christine Larré-Larrouy; Christian Feller; Carmen Silvia Vieira Janeiro Neves

    2005-01-01

    Soil organic matter (SOM) is an important component of agriculture sustainability. A clayey oxisol was studied by SOM particle size fractions in a citrus orchard managed (for a 9 year period) with a) Arachis prostrata ground cover; b) spontaneous vegetation controlled by mowing and tillage; c) bare soil. Also the same soil was studied under a 20 year period of annual crops (conventional tillage) and native forest. The orchard ground covers increased the soil C and N stocks when compared to or...

  9. Effects of plant diversity on plant biomass production and soil macrofauna in Amazonian pastures

    OpenAIRE

    Laossi, Kam-Rigne; Barot, Sébastien; Carvalho, D; Desjardins, Thierry; Lavelle, Patrick; Martins, M.; Mitja, Danielle; Rendeiro, A. C.; Rousseau, G.; Sarrazin, Max; Velasquez, Elena; Grimaldi, Michel

    2008-01-01

    We examined the effect of plant diversity on plant production and soil macrofauna density and diversity. Four plants species (Arachis pintoi, an herbaceous legume; Brachiaria brizantha, a perennial grass; Leucaena leucocephala, a legume shrub; Solanum rugosum, a non-legume shrub) were used in a field experiment and communities of all combinations of one, two, three or four species were established. Plant diversity neither significantly affected density and diversity of soil macrofauna nor tot...

  10. Pengaruh Pemberian Berbagai Tingkat Mikoriza Arbuskula pada Tanah Ultisol terhadap Produktivitas Leguminosa

    OpenAIRE

    Mustapa, Andrian

    2015-01-01

    Soil ultisol given arbuscular mycorrhizal fungi increased the nutrient content of the soil thereby increased the productivity of legume. The objective of this research to determine the response of various levels of arbuscular mycorrhizal fungi in the soil ultisol on productivity of legume (Arachis glabrata, Centrosema pubescens and Pueraria javanica) as measured by the production of fresh, dry matter production and root biomass. The research conducted at field trial Animal Husbandry Depar...

  11. Starch grains on human teeth reveal early broad crop diet in northern Peru

    OpenAIRE

    Piperno, Dolores R.; Dillehay, Tom D.

    2008-01-01

    Previous research indicates that the Ñanchoc Valley in northern Peru was an important locus of early and middle Holocene human settlement, and that between 9200 and 5500 14C yr B.P. the valley inhabitants adopted major crop plants such as squash (Cucurbita moschata), peanuts (Arachis sp.), and cotton (Gossypium barbadense). We report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultiva...

  12. Physicochemical Properties and Fungitoxicity of the Essential Oil of Citrus medica L. against Groundnut Storage Fungi

    OpenAIRE

    ESSIEN, Emmanuel Peter; ESSIEN, Joseph Peter; Ita, Basil Nse

    2008-01-01

    The in vitro antifungal effect of the essential oil of Citrus medica L. on storage fungi of Arachis hypogea L. stored for 6 months was evaluated using the disc diffusion agar method. The oil exhibited a wide spectrum of fungitoxicity, inhibiting all 14 fungus species tested. Thus, the oil can be exploited as a fumigant against storage fungi for the preservation of stored legume seeds due to its wide range of activity, non-phytotoxicity, and long-term persistence of fungitoxicity.

  13. Características morfológicas e produtivas de leguminosas forrageiras tropicais submetidas a duas frequências de corte Morphologic and productive characteristics of tropical forage legumes under two harvest frequencies

    OpenAIRE

    Valdson José da Silva; José Carlos Batista Dubeux Junior; Vicente Imbroisi Teixeira; Mércia Virgínia Ferreira dos Santos; Mario de Andrade Lira; Alexandre Carneiro Leão de Mello

    2010-01-01

    Objetivou-se com este trabalho avaliar características morfológicas e produtivas de leguminosas forrageiras submetidas a duas frequências de corte (28 e 56 dias) a altura de 10 cm. Foram avaliadas as seguintes espécies: Arachis pintoi (cv. Amarillo), Clitoria ternatea, Calopogonium mucunoides, Desmodium ovalifolium (cv. Itabela) e Stylosanthes guianensis (cvs. Bandeirante, Cook, Mineirão). O delineamento utilizado foi o inteiramente casualizado em arranjo fatorial (7 leguminosas × 2 freq...

  14. In situ caecal degradation of roughages in horses Degradação cecal in situ de alimentos volumosos em equinos

    OpenAIRE

    Vinícius Pimentel Silva; Fernando Queiroz de Almeida; Eliane da Silva Morgado; Liziana Maria Rodrigues; Tiago Marques dos Santos; Henrique Torres Ventura

    2010-01-01

    The present study was carried out to evaluate the in situ degradation of dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP) in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa), peanut (Arachis pintoi cv. Amarillo), desmodio(Desmodium ovalifolium), stylo (Stylosanthes guianensis cv. Mineirão), pigeon pea (Cajanus cajan), lime-yellow pea (Macrotyloma axillare) and coastcross hay (Cynodon dactylon cv. co...

  15. Digestibilidade dos nutrientes de alimentos volumosos determinada pela técnica dos sacos móveis em eqüinos Nutrient digestibility of forage feed determined using mobile bag technique in horses

    OpenAIRE

    Vinícius Pimentel Silva; Fernando Queiroz de Almeida; Eliane da Silva Morgado; Almira Biazon França; Henrique Torres Ventura; Liziana Maria Rodrigues

    2009-01-01

    Objetivou-se estimar a digestibilidade de nutrientes de forrageiras em eqüinos utilizando-se a técnica de sacos de náilon móveis. Foram avaliados alfafa (Medicago sativa), amendoim forrageiro (Arachis pintoi), desmódio (Desmodium ovalifolium), estilosantes (Stylosanthes guianensis), guandu (Cajanus cajan), macrotiloma (Macrotyloma axillare) e capim-coastcross (Cynodon dactylon cv. coastcross). O delineamento foi em blocos inteiramente casualizados com sete alimentos e cinco blocos (animais). ...

  16. Nutrient Bag Breeding Technology of Pinto peanut in Autumn%优良绿肥品种平托花生秋季营养袋繁育技术

    Institute of Scientific and Technical Information of China (English)

    吴光明; 林昭政; 林大铨

    2011-01-01

    Nutrient bag breeding technology of Pinto peanut(Arachis pintoi) in autumn was summarized,including preparation and bagging of nutritive soil,preparation and cutting of breeding material as well as seed bed management.%总结了平托花生秋季营养袋繁育技术,主要包括营养土的配制和装袋、繁育材料准备和扦插、苗床管理。

  17. LLuvia, Escurrimiento Superficial y Erosión del Suelo en Sistemas Agroforestales de café bajo sombra

    OpenAIRE

    Víctor Ordaz-Chaparro; Joel Pérez-Nieto; Eduardo Valdés-Velarde; Matías E. Hernández-San Román

    2005-01-01

    En una región tropical de Veracruz, México, se evaluaron sin repetición cinco sistemas agroforestales de café bajo sombra: 1) sistema agroforestal rusticano; 2) policultivo tradicional mejorado (PTM) con barreras vivas de palo de agua (Eupatorium morifolium Mill) y terrazas individuales; 3) PTM con cobertura de canavalia (Canavalia ensiformis (L) De Candolle); 4) PTM con cobertura de cacahuatillo (Arachis pintoi Krap Greg nom. nud. (Cook)); 5) PTM con barreras vivas de palma camedor (Chamae...

  18. ESTABLISHMENT TECHNIQUES FOR TROPICAL LEGUMES IN THE UNDERSTORY OF A EUCALYPTUS PLANTATION

    Directory of Open Access Journals (Sweden)

    Maria Luiza Franceschi Nicodemo

    2015-04-01

    Full Text Available This study evaluated establishment methods for a mixture of herbaceous forage legumes [Centrosema acutifolium, Clitoria ternatea, Pueraria phaseoloides, Stylosanthes Campo Grande (Stylosanthes capitata + S. macrocephala, Calopogonium mucunoides, Lablab purpureus, Arachis pintoi, and Aeschynomene villosa] under the shade of an Eucalyptus grandis plantation submitted to thinning (40% 8 years after planting in Anhembi, São Paulo (22°40'S, 48°10'W, altitude of 455 m. The experiment started in December 2008 and consisted of the comparison of the following four types of seed incorporation by light disc harrowing: (1 broadcast sowing without seed incorporation; disc harrowing before (2 or after (3 planting, and (4 disc harrowing before and after planting. Ninety days after planting, the number of legume plants/m2 and the percentage of ground cover by the plants varied between the treatments tested; however, the treatments had no effect on the dry matter accumulation of forage legumes. Disc harrowing before planting yielded superior results compared to the treatments without disc harrowing and disc harrowing after planting. At the end of the experimental period, the plots contained Arachis, Centrosema, Stylosanthes, and Pueraria. The dry matter accumulated by Centrosema corresponded to 73% of total dry matter yield of the plots. The participation of Arachis, Centrosema and Stylosanthes in final dry matter composition of the plots varied according to establishment method. The advantages of the use of species mixtures rather than monocultures in the understory of forest plantations were discussed.

  19. Características morfológicas e produtivas de leguminosas forrageiras tropicais submetidas a duas frequências de corte Morphologic and productive characteristics of tropical forage legumes under two harvest frequencies

    Directory of Open Access Journals (Sweden)

    Valdson José da Silva

    2010-01-01

    Full Text Available Objetivou-se com este trabalho avaliar características morfológicas e produtivas de leguminosas forrageiras submetidas a duas frequências de corte (28 e 56 dias a altura de 10 cm. Foram avaliadas as seguintes espécies: Arachis pintoi (cv. Amarillo, Clitoria ternatea, Calopogonium mucunoides, Desmodium ovalifolium (cv. Itabela e Stylosanthes guianensis (cvs. Bandeirante, Cook, Mineirão. O delineamento utilizado foi o inteiramente casualizado em arranjo fatorial (7 leguminosas × 2 frequências de corte com quatro repetições, para avaliação das seguintes variáveis: acúmulo de biomassa, número de ramificações/planta, número de folhas vivas/planta, massa seca das raízes, número e massa seca dos nódulos. A produção acumulada de MS da parte aérea e das raízes foi equivalente para os cortes efetuados a cada 28 dias ou a cada 56 dias, com exceção do Arachis, Clitoria e Desmodium, que apresentaram maior biomassa aérea e de raízes no intervalo de corte de 56 dias. Houve diferenças entre leguminosas quanto à massa seca e ao número de nódulos, todavia, o maior número de nódulos foi observado na frequência de 56 dias. O número de folhas vivas/planta foi maior na frequência de 56 dias, com exceção das leguminosas Arachis e Calopogonium, cujos valores foram próximos quando cortadas nas diferentes frequências. A frequência de corte afetou de forma diferenciada as características morfológicas e produtivas das leguminosas estudadas, o que indica a necessidade de manejo diferenciado para as variedades testadas.The objective of this research was to evaluate morphological and productive characteristics of forage legumes under two harvest frequencies (28 and 56 days and 10 cm harvest intensity. The following legume species were evaluated: Arachis pintoi (cv. Amarillo, Clitoria ternatea, Calopogonium mucunoides, Desmodium ovalifolium (cv. Itabela and Stylosanthes guianensis (cvs. Bandeirante, Cook, Mineirão. A randomized

  20. Produção de híbridos de amendoim forrageiro por meio de hibridação artificial Production of forage peanut hybrids through artificial hybridization

    Directory of Open Access Journals (Sweden)

    Marilda Augusta Peres Oliveira

    2002-06-01

    Full Text Available O objetivo deste trabalho foi a obtenção de híbridos de amendoim forrageiro por meio da hibridação artificial. O experimento foi realizado na Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, durante a época de florescimento dos acessos de Arachis pintoi Krap. & W. C. Gregory e de A. repens Handro. Cerca de 700 polinizações produziram 27 segmentos de frutos, com taxas de fecundação que variaram entre 1,1 e 12,9%, considerando-se todas as combinações híbridas. Os híbridos intra-específicos de A. pintoi produziram sementes F2, e os interespecíficos não produziram semente. A técnica de hibridação utilizada nas espécies forrageiras necessitou de ajustes, devido a diferenças observadas em relação ao amendoim cultivado, entre elas o hábito de crescimento.The purpose of this work was to obtain forage peanut hybrids through artificial hybridization. The experiment was conducted in a screenhouse at Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia during the flowering period of Arachis pintoi Krap. & W. C. Gregory and A. repens Handro accessions. About 700 pollinations produced 27 fruit segments and the fertilization rates ranged from 1.1 to 12.9% for all cross combinations. The intraspecific hybrids produced F2 seeds, which did not occur to the interspecific hybrids. To effect the hybridization technique, adjustments were necessary to forage Arachis species, in relation to cultivated peanut, since differences in the growth habit were verified.

  1. Evaluación del riesgo agroambiental de los suelos de las comunidades indígenas del estado Anzoátegui, Venezuela

    OpenAIRE

    Lugo Morin, Diosey Ramón

    2007-01-01

    Con el propósito de realizar una evaluación del riesgo agroambiental en los suelos de las comunidades indígenas del Estado Anzoátegui Venezuela, se evaluaron cinco unidades de tierra de la zona de interés frente a diez usos agropecuarios; Algodón (Gossypium hirsutum), Fríjol (Vigna sinensis), Maíz (Zea mays), Maní (Arachis hypogea), Melón (Cucumis melo), Ñame (Dioscorea alata), Ganadería (pasto), Patilla (Citrullus vulgaris), Sorgo (Sorghum bicolor) y Yuca (Manihot esculenta). La ...

  2. Evaluación del riesgo agroambiental de los suelos de las comunidades indígenas del estado Anzoátegui, Venezuela

    OpenAIRE

    D. R. Lugo-Morin

    2007-01-01

    Evaluación del riesgo agroambiental de los suelos de las comunidades indígenas del estado Anzoátegui, Venezuela. Con el propósito de realizar una evaluación del riesgo agroambiental en los suelos de las comunidades indígenas del Estado Anzoátegui Venezuela, se evaluaron cinco unidades de tierra de la zona de interés frente a diez usos agropecuarios; Algodón (Gossypium hirsutum), Fríjol (Vigna sinensis), Maíz (Zea mays), Maní (Arachis hypogea), Melón (Cucumis melo), Ñame (Dioscorea al...

  3. Effect of oils on drug absorption

    OpenAIRE

    Palin, K.J.

    1981-01-01

    Oil and emulsion vehicles have been shown to alter the oral absorption of many drugs. This may be due to enhanced lymph flow and/or altered gastro-intestinal motility in the presence of the oils. The oral absorption of a model compound (DOT) in the presence of three chemically different oils, arachis oil, Miglyol 812 and liquid paraffin was investigated in rats, the influence of lymphatic absorption and gastro-intestinal motility being determined. The findings were applied to the for.mulat...

  4. Aislamiento de "Micromonospora" de nódulos de leguminosas tropicales y análisis de su interés como promotor del crecimiento vegetal

    OpenAIRE

    Cerda Castillo, María Eugenia

    2008-01-01

    En el presente trabajo se plantea el aislamiento, caracterización e identificación de cepas de Micromonospora aisladas de nódulos fijadores de nitrógeno de las leguminosas tropicales Phaseolus vulgaris y Arachis hypogeae recogidas en Nicaragua y que son de importancia agrícola en toda América Latina. Posteriormente se realizarán experimentos de reinoculación en planta con el fin de ampliar nuestros conocimientos sobre el papel que pueden desempeñar estos microorganismos en la nodulación y fij...

  5. Producción de semilla de cacahuate forrajero con siete dosis de cal y tres fechas de cosecha

    OpenAIRE

    Javier Francisco Enríquez Quiroz; Adrián Raymundo Quero Carrillo

    2001-01-01

    Arachis pintoi (cacahuatillo) es una valiosa leguminosa que puede ser utilizada con diferentes propósitos. En México la producción de semilla es nula, así como su manejo. Los objetivos fueron determinar la respuesta a la aplicación de cal y el tiempo a la cosecha de semilla. Se evaluaron dos ecotipos del CIAT (17434 y 18744), siete niveles de cal y tres fechas de cosecha, en un diseño de parcelas divididas; Se registró información de producción de semilla, peso unidad de semilla sin lavar, la...

  6. Análisis patogénico y genético de Macrophomina phaseolina (Tassi) Goid. en diferentes hospedantes

    OpenAIRE

    Rosa María Muñoz Cabañas; Sanjuana Hernández Delgado; Netzahualcóyotl Mayek Pérez

    2005-01-01

    Se determinó la diversidad patogénica de 30 aislamientos y genética en 20 aislamientos del hongo Macrophomina phaseolina obtenidos de México y otros países. La patogenicidad se determinó in vitro en dos variedades de seis especies de plantas (maíz, Zea mays; frijol, Phaseolus vulgaris; soya, Glycine max; cacahuate, Arachis hypogea; ajonjolí, Sesamum indicum; garbanzo, Cicer arietinum) y el genotipo se determinó con base en el análisis AFLP (polimorfismos en la longitud de los fragmentos ampli...

  7. Influence of Mucilage Viscosity On The Globule Structure And Stability Of Certain Starch Emulsions

    OpenAIRE

    Uhumwangho MU; Okor RS; Ayomanor M

    2005-01-01

    A study was carried out to determine the influence of mucilage viscosity on the globule structure (i.e. size and number) of certain starch emulsions. The starches investigated were cassava, potato and maize. The emulsions were prepared by mixing the starch mucilage of a predetermined concentration 4%w/v with arachis oil in the ratio 50:50, using a silverson mixer fitted with a dispersator head. The emulsions were stored at room temperature (28±20C) for 7 days. Changes in globule size were mon...

  8. Synthesis and non-covalent functionalization of carbon nanotubes rings: new nanomaterials with lectin affinity

    Science.gov (United States)

    Assali, Mohyeddin; Pernía Leal, Manuel; Fernández, Inmaculada; Khiar, Noureddine

    2013-03-01

    We present a mild and practical carbon nanotubes rings (CNRs) synthesis from non-covalent functionalized and water-soluble linear single-wall carbon nanotubes. The hemi-micellar-supramolecular self-organization of lactose-based glycolipid 1 on the ring surface, followed by photo-polymerization of the diacetylenic function triggered by UV light afforded the first water-soluble and biocompatible CNRs. The obtained donut-like nanoconstructs expose a high density of lactose moieties on their surface, and are able to engage specific interactions with Arachis hypogea lectin similar to glycoconjugates on the cell membrane.

  9. Synthesis and non-covalent functionalization of carbon nanotubes rings: new nanomaterials with lectin affinity

    International Nuclear Information System (INIS)

    We present a mild and practical carbon nanotubes rings (CNRs) synthesis from non-covalent functionalized and water-soluble linear single-wall carbon nanotubes. The hemi-micellar–supramolecular self-organization of lactose-based glycolipid 1 on the ring surface, followed by photo-polymerization of the diacetylenic function triggered by UV light afforded the first water-soluble and biocompatible CNRs. The obtained donut-like nanoconstructs expose a high density of lactose moieties on their surface, and are able to engage specific interactions with Arachis hypogea lectin similar to glycoconjugates on the cell membrane. (paper)

  10. Cultivo orgânico de coentro em plantio direto utilizando cobertura viva e morta adubado com composto Organic faming of coriander in no-tillage system fertilized with compost using dead and living mulching

    OpenAIRE

    Leonardo Barreto Tavella; Robson de Oliveira Galvão; Regina Lúcia Félix Ferreira; Sebastião Elviro de Araújo Neto; Jacson Rondinelle da Silva Negreiros

    2010-01-01

    O objetivo deste trabalho foi avaliar o desempenho agronômico do coentro em sistema de plantio direto orgânico sob diferentes tipos de cobertura viva e palhada e doses crescentes de composto orgânico. Foi utilizado o delineamento em blocos aleatorizados em esquema de parcela subdividida com quatro repetições. As parcelas corresponderam aos sistemas de plantio direto com cobertura viva de Arachis pintoi, cobertura viva de plantas espontâneas e cobertura com palhada de resteva natural que foram...

  11. Produção de laranja com plantas de cobertura permanente na entrelinha Orange yield in orchard floor vegetation management

    OpenAIRE

    Jonez Fidalski; Celso Jamil Marur; Pedro Antonio Martins Auler; Cássio Antonio Tormena

    2006-01-01

    O objetivo deste trabalho foi avaliar as relações hídricas, os parâmetros fisiológicos e a produção de frutos da laranjeira 'Pêra' enxertada sobre o limoeiro 'Cravo', assim como a umidade do solo, em diferentes plantas de cobertura permanente na entrelinha. O experimento de campo foi implantado em 1993, em Argissolo Vermelho distrófico latossólico, no Município de Alto Paraná, noroeste do Paraná. Foram avaliados três tratamentos: gramínea (Paspalum notatum), leguminosa (Arachis pintoi) e test...

  12. Influência da luminosidade no comportamento de onze espécies forrageiras perenes de verão Influence of luminosity on the behavior of eleven perennial summer forage species

    OpenAIRE

    André Brugnara Soares; Laércio Ricardo Sartor; Paulo Fernando Adami; Alexandre Costa Varella; Lidiane Fonseca; Jean Carlos Mezzalira

    2009-01-01

    Objetivou-se avaliar o comportamento de espécies forrageiras (Brachiaria decumbens cv. Basilisk, Brachiaria brizantha cv. Marandu, Panicum maximum cvs. Tanzânia, Aruana e Mombaça, Hemarthria altissima cv. Florida; Paspalum notatum cv. Pensacola, Axonopus catharinensis, Cynodon sp. híbrido Tifton-85; Arachis pintoi cvs. Alqueire e Amarillo) submetidas a diferentes níveis de luminosidade produzidos por árvores de Pinus taeda (céu aberto; 9 m entre linhas e 3 m entre árvores; e 15 m entre linhas...

  13. Decomposição e liberação de nutrientes acumulados em leguminosas herbáceas perenes consorciadas com bananeira Decomposition and nutrient release of perennial herbaceous legumes intercropped with banana

    OpenAIRE

    José Antonio Azevedo Espindola; José Guilherme Marinho Guerra; Dejair Lopes de Almeida; Marcelo Grandi Teixeira; Segundo Urquiaga

    2006-01-01

    A avaliação da decomposição dos resíduos vegetais adicionados ao solo pelas plantas de cobertura permite melhor compreensão do fornecimento de nutrientes para as culturas de interesse comercial. O presente estudo foi realizado no campo com o objetivo de avaliar a decomposição e a liberação de nutrientes pela parte aérea de leguminosas herbáceas perenes. Os tratamentos consistiram de diferentes plantas de cobertura do solo consorciadas com bananeira: amendoim forrageiro (Arachis pintoi Krapov....

  14. Decomposição e liberação de nutrientes de resíduos culturais de plantas de cobertura em argissolo vermelho-amarelo na região noroeste Fluminense (RJ) Decomposition and nutrient release from cover crop residues in passion-fruit plantation

    OpenAIRE

    Antonio Carlos da Gama-Rodrigues; Emanuela Forestieri da Gama-Rodrigues; Elio Cruz de Brito

    2007-01-01

    A decomposição pode assumir importante papel no manejo da fertilidade do solo, possibilitando a elaboração de técnicas de cultivo que melhorem a utilização de nutrientes contidos nos resíduos vegetais. O objetivo deste trabalho foi estimar as taxas de decomposição e liberação de C, N, P, K, Ca e Mg de resíduos culturais provenientes de plantas de coberturas na cultura do maracujá. As espécies avaliadas foram feijão-de-porco (Canavalia ensiformis), amendoim forrageiro acesso CIAT 1734 (Arachis...

  15. Produção de mudas de espécies forrageiras no sistema hidropônico de leito flutuante (floating) com solução nutritiva à base de biofertilizante ou adubo solúvel = Production of seedlings of forage species in floating hydroponics system with biofertilizer or soluble fertilizer

    OpenAIRE

    Ricardo Probst; Sérgio Augusto Ferreira de Quadros; Júlio Graeff Erpen; Mário Luiz Vincenzi

    2009-01-01

    Neste trabalho objetivou-se avaliar a sobrevivência das estacas e a produção de matéria seca na fase de cultivo de mudas das espécies forrageiras missioneira gigante (Axonopus catharinensis), amendoim forrageiro (Arachis pintoi) e maku (Lotus uliginosus cv. Maku). No sistema hidropônico de leito flutuante com solução nutritiva à base de biofertilizante ou adubo solúvel. O delineamento experimental adotado foi o de blocos ao acaso em esquema fatorial 3 x 2, sendo três espécies forrageiras e du...

  16. Perfil de n-alcanos em cinco espécies de plantas forrageiras tropicais - DOI: 10.4025/actascianimsci.v27i3.1207 Profile of n-alkanes in five species of plants tropical forages - DOI: 10.4025/actascianimsci.v27i3.1207

    OpenAIRE

    Antônio Ferriani Branco; Eduardo Shiguero Sakaguti; Nelson Massaru Fukumoto; Júlio Cesar Damasceno; Cristiano Côrtes; Ulisses Cecato

    2005-01-01

    O objetivo do experimento foi estudar o perfil de n-alcanos em espécies de gramíneas (Brachiaria brizantha, Cynodon dactylon e Panicum maximum) e leguminosas (Arachis pintoi e Glycine wightii). Foram identificados e quantificados por meio de cromatografia gasosa, os n-alcanos C24 a C35, sendo C32 e C34 padrões internos. As concentrações dos n-alcanos nas diferentes espécies e respectivas frações (lâminas foliares, colmos porções superior e inferior e matéria morta para gramíneas; folhas, caul...

  17. Dicty_cDB: Contig-U13346-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available in OTTH059... 82 5e-14 DQ887084_1( DQ887084 |pid:none) Arachis hyp...e) African swine fever virus OURT 8... 62 5e-08 DQ848992_1( DQ848992 |pid:none) Trachemys scripta ubiquitin-...f sntlrhtktnnyftkpttttttttttnnsttknnfkpihhsn**ndcnfiypscgtchke iiflrkiiikikikilhkiekypqyicdhfksif Frame C: Y... magnipapillata cD... 56 1e-17 3 ( CF655363 ) tac88e02.y1 Hydra EST -IV Hydra magnipapillata cD....ntea from female go... 92 3e-23 3 ( BQ103759 ) ESTBB170018B10E01.3 Bee Brain Normalized/Subtract... 82 8e-18

  18. Potential of tropical plants to exert defaunating effects on the rumen and to reduce methane production

    International Nuclear Information System (INIS)

    This paper summarises the principal results obtained in Cuba concerning the potential of different tropical plants to exert defaunating effects in the rumen and to reduce methane (CH4) production. The plants studied were Sapindus saponaria, Morus alba, Trichanthera gigantea, Tithonia diversifolia, Gliricidia sepium Leucaena leucocephala, Stizolobium aterrimun and Arachis pintoi. Grasses used as forage in the assays to obtain grass:foliage mixtures were Pennisetum purpureum Cuba CT-115 or Star grass (Cynodon nlemfuensis). The experiments were conducted using an in vitro system. Gases produced in the fermentation process were collected at intervals of 4, 8, 12 and 24 h and CH4 production was determined by gas chromatography. Phytochemical analyses indicated the presence of tannins, saponins and others secondary compounds. Enterolobium and Leucaena had a high content of tannins and moderate levels of saponins. Morus contained moderate amounts of saponins. The inclusion of 15% Leucaena and Gliricidia, 20% Sapindus and Arachis as well as 40% S. aterrimum, negatively affected protozoal populations. The inclusion of 25% Sapindus, Morus and Trichantera foliages using P. purpureum as the pasture base reduced CH4 production significantly. The results suggest that the use of trees and shrubs to supplement low quality forages seems appropriate for reducing CH4 production and improving animal nutrition in tropical areas. (author)

  19. Starch grains on human teeth reveal early broad crop diet in northern Peru.

    Science.gov (United States)

    Piperno, Dolores R; Dillehay, Tom D

    2008-12-16

    Previous research indicates that the Nanchoc Valley in northern Peru was an important locus of early and middle Holocene human settlement, and that between 9200 and 5500 (14)C yr B.P. the valley inhabitants adopted major crop plants such as squash (Cucurbita moschata), peanuts (Arachis sp.), and cotton (Gossypium barbadense). We report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivated squash and peanuts along with two other major food plants not previously detected. Starch from the seeds of Phaseolus and Inga feuillei, the flesh of Cucurbita moschata fruits, and the nuts of Arachis was routinely present on numerous teeth that date to between 8210 and 6970 (14)C yr B.P. Early plant diets appear to have been diverse and stable through time and were rich in cultivated foods typical of later Andean agriculture. Our data provide early archaeological evidence for Phaseolus beans and I. feuillei, an important tree crop, and indicate that effective food production systems that contributed significant dietary inputs were present in the Nanchoc region by 8000 (14)C yr B.P. Starch grain studies of dental remains document plants and edible parts of them not normally preserved in archaeological records and can assume primary roles as direct indicators of ancient human diets and agriculture. PMID:19066222

  20. Weed supression by smother crops and selective herbicides

    Directory of Open Access Journals (Sweden)

    Severino Francisco José

    2004-01-01

    Full Text Available Using a smother crop is thought to suppress weed density and to add other beneficial effects in sustainable agricultural systems. Weed suppression ought to be considered an essential component of integrated weed management. However, very little is known about the effects of green manure plants on weeds. This study evaluated the influence of three green manure species on weed suppression and selectivity of herbicides. A field experiment was designed to determine the effect of the green manure species Crotalaria juncea, Arachis pintoi and pigeon pea on the weeds Brachiaria decumbens, guineagrass and hairy beggarticks, and on the natural weed infestation in the inter rows area of an avocado orchard. The weed species were suppressed differently by each green manure species. Soil samples collected from the field experiment presented a residual effect, of at least 30 d, in suppressing weed seed bank recruitment; this residual effect was caused by the residues of the green manure present in the soil. When the green manure was incorporated into the top 5 cm of soil or left on the surface, in a greenhouse experiment, the emergence of weed seeds was significantly inhibited, depending on the species, and on the amount and depth of green manure incorporation. Greenhouse experiments indicate that pre-emergence herbicides cause lower phytotoxicity than post-emergence Arachis pintoi. Smother crops using green manure species, when well established in an area, provide additional weed control to the cropping system and are effective and valuable tools in integrated weed management.

  1. Effect of Pollen Feed on Parasitization and Predatism of Cephalonomia stephanoderis onHypothenemus hampei

    Directory of Open Access Journals (Sweden)

    Dwi Suci Rahayu

    2014-05-01

    Full Text Available Biological control of the coffee berry borer (Hypothenemus hampeiusing parasitoid Cephalonomia stephanoderishas been developed through the improvement of the parasitoid role may using pollens as feed source. The objective of this study was to investigate the effect of cover crop and weed pollens on parasitization and predatism of C. stephanoderis.The applied treatments were pollens of Turnera ulmifolia, Arachis pintoi, Ageratum conyzoidesadded in glass tube that consist of 10 CBB pupaes and a mated female of C. stephanoderis. Number of pupae parasitized and pupae preyed were observed. The result showed that addition of A. Pintoi pollen increased the number of pupae parasitized at 135% whereas addition of T. ulmifolia and A. conyzoides pollens did not affect parasitization of C. Stephanoderis. The predatismof C. stephanoderiswas higher than parasitization to pupae of H. hampei which showed that the behavior of C. stephanoderiswas parasitization. Addition of T. ulmifolia, A. pintoi, and A. conyzoidespollens increased the number of pupae predatism at 132%, 102%, and 225%, respectively. Key words: Ageratum conyzoides, Arachis pintoi, Cephalonomia stephanoderis, Hypothenemus hampei,parasitization, predatism, pollens, Turnera ulmifolia

  2. Biomass accumulation and chemical composition of Massai grass intercropped with forage legumes on an integrated crop-livestock-forest system

    Directory of Open Access Journals (Sweden)

    Tatiana da Costa Moreno Gama

    2014-06-01

    Full Text Available The objective was to evaluate the use of woody legumes (Albizia lebbeck, Cratylia argentea, Dipteryx Allata (Baru, a Leucaena hybrid (L. leucocephala + L. diversifolia, and Leucaena leucocephalacv. Cunningham and herbaceous legumes (Arachis pintoi intercropped with Panicum maximum cv. Massai, simultaneously implanted in a maize crop. The study made use of a randomized block experimental design with four replications. Assessments of biomass accumulation and forage nutritional value were made after the maize harvest, between June 2008 and October 2010. It was found that the residues of maize provided better growing conditions for Massai grass during the dry season. L. leucocephala cv. Cunningham and the Leucaena hybrid had the highest accumulation of all forage legumes evaluated, and provided the best nutritional value of all the arrangements tested. Of all woody legumes tested in this system, Leucaena was considered feasible for intercropping with Massai grass. The intercrop of perennial woody Baru with maize is not recommended. Albizia lebbeck and Cratylia argentea require further study, especially the yield assessment at different cutting intervals and cutting heights. Arachis pintoi had a low participation in the intercropping, showing greater performance over time, indicating slow thriving in this experimental condition.

  3. Influence of sodium chloride and sodium sulfate salinities on photosynthetic carbon assimilation in peanut

    International Nuclear Information System (INIS)

    The effect of NaCl and Na2SO4 treatments on chlorophyll content, rate of 14C assimilation and products of photosynthesis in peanut (Arachish hypogaea L.) variety TMV-10 has been investigated. It was observed that chlorophyll content was affected mainly by NaCl, Na2SO4 treatment lowered the rate of photosynthetic 14CO2 fixation. The analysis of labelled products revealed that the salts affect the carbon metabolism differently. The radioactivity was found to be accumulated in fractions of sugars and sugarphosphates in the leaves of NaCl treated plants. Na2SO4 treatment brought about considerable decline in labelling of sugars and an increase in labelling of amino acids and sugarphosphates. (orig.)

  4. The taxonomic position of Roesleria subterranea.

    Science.gov (United States)

    Kirchmair, Martin; Neuhauser, Sigrid; Buzina, Walter; Huber, Lars

    2008-10-01

    The genus Roesleria was introduced with the single species Roesleria hypogaea (current name: R. subterranea) by Thümen in 1877. The species was originally described from roots of grapevine and recognised as a facultative root-rotting parasite. The mazaediate ascoma with evanescent asci led to the assumption that Roesleria would be an ally of mazaediate lichens. In this study we calculate 28S, 18S as well ITS1-5.8S-ITS2 rDNA phylogenies. These data indicate that Roesleria is closely related to Hymenoscyphus (Helotiales). In contrast to other members of the Helotiales, the ascospores of Roesleria are passively released, and a distinct apical apparatus cannot be observed by LM. Electron optic investigations have also not elucidated whether the asci of Roesleria are equipped with a rudimentary apical apparatus or not. The passive release of the ascospores is discussed as an apomorphic character that evolved as adaptation to a hypogeous living. PMID:18693105

  5. Velocidade de estabelecimento de acessos de amendoim forrageiro na Amazônia Ocidental Speed of establishment of accessions of forage peanut in the Western Amazon

    Directory of Open Access Journals (Sweden)

    Judson Ferreira Valentim

    2003-12-01

    Full Text Available O objetivo deste estudo foi avaliar a velocidade de estabelecimento de acessos de amendoim forrageiro (Arachis repens e Arachis pintoi, visando selecionar materiais adaptados aos sistemas intensivos de produção pecuária do Acre. O delineamento experimental utilizado foi de blocos casualizados, com quatro repetições. Os tratamentos consistiram de dois acessos de A. repens, sete acessos e duas cultivares de Arachis pintoi identificados como promissores para as condições ambientais de Rio Branco, Acre. Foi adotado como testemunha A. pintoi cv. Amarillo. Os acessos Ap 65, Ap 39 e Ar 10, com desempenho semelhante às cultivares Amarillo e Belmonte, destacaram-se por apresentar excelente velocidade de estabelecimento, com índice de sobrevivência das mudas e cobertura do solo superiores a 80% e comprimento dos estolões acima de 85 cm, respectivamente, aos 50, 70 e 120 dias após o plantio. Estes genótipos apresentaram produtividade de matéria seca (MS superior a 2.300 kg/ha, taxas de acúmulo de MS iguais ou superiores a 20 kg/ha/dia e teor de proteína bruta variando entre 17,9 e 21,7%, no final do período de estabelecimento. Entre os quatro grupos heteróticos, o formado pelo acesso Ap 39 destacou-se dos demais, por apresentar valores médios a altos para todas as características avaliadas, de acordo com a análise de agrupamento realizada pelo Método de Otimização de Tocher, com base na distância generalizada de Mahalanobis. Para que os materiais promissores possam ser recomendados para uso nos sistemas intensivos de produção de bovinos no Acre, devem ser desenvolvidos estudos adicionais com relação à: 1 produtividade e qualidade de MS nos períodos chuvoso e seco; 2 ocorrência de pragas e doenças; 3 produção de sementes; 4 adaptação a solos de baixa permeabilidade; 5 compatibilidade com gramíneas forrageiras e espécies arbóreas e arbustivas perenes; 6 produção animal e persistência sob pastejo.The objective of

  6. Produção de mudas de espécies forrageiras no sistema hidropônico de leito flutuante (floating com solução nutritiva à base de biofertilizante ou adubo solúvel - DOI: 10.4025/actascianimsci.v31i4.4290 Production of seedlings of forage species in floating hydroponics system with biofertilizer or soluble fertilizer - DOI: 10.4025/actascianimsci.v31i4.7459

    Directory of Open Access Journals (Sweden)

    Júlio Graeff Erpen

    2009-10-01

    Full Text Available Neste trabalho objetivou-se avaliar a sobrevivência das estacas e a produção de matéria seca na fase de cultivo de mudas das espécies forrageiras missioneira gigante (Axonopus catharinensis, amendoim forrageiro (Arachis pintoi e maku (Lotus uliginosus cv. Maku. No sistema hidropônico de leito flutuante com solução nutritiva à base de biofertilizante ou adubo solúvel. O delineamento experimental adotado foi o de blocos ao acaso em esquema fatorial 3 x 2, sendo três espécies forrageiras e duas soluções nutritivas. As espécies não apresentaram diferença quanto à sobrevivência (p = 0,225, independentemente do tipo de fertilizante (p = 0,92. No entanto, quando se quantificou a produção de MS planta-1 proporcionada por cada uma dessas espécies, o maku (p = 0,001 obteve as maiores quantidades (47,18 g, enquanto o amendoim forrageiro (19,90 g e a missioneira gigante (16,81 g foram semelhantes entre si (p = 0,227, tendo o mesmo ocorrido entre os fertilizantes (p = 0,559. Deste modo, as três espécies possuem condições semelhantes de sobrevivência, independentemente da concentração de nutrientes da solução nutritiva, com o maku proporcionando a maior produção de MS planta-1.Production of seedlings of forage species in floating hydroponics system with biofertilizer or soluble fertilizer. The aim of this work was to evaluate the survival and dry matter production during the seedling culture phase of three forage species: Axonopus catharinensis, forage peanut (Arachis pintoi, and greater lotus (Lotus uliginosus cv. Maku, in a floating hydroponic system using biofertilizer or soluble fertilizer. The experimental design adopted was randomized blocks in a 3 x 2 factorial scheme, with three forage species and two fertilizers. There was no difference between species with regard to survival (p = 0.225, regardless of the fertilizer used (p = 0.92. However, when dry matter production was considered, Maku (p = 0,001 showed greater

  7. Produção de mudas de espécies forrageiras no sistema hidropônico de leito flutuante (floating com solução nutritiva à base de biofertilizante ou adubo solúvel = Production of seedlings of forage species in floating hydroponics system with biofertilizer or soluble fertilizer

    Directory of Open Access Journals (Sweden)

    Ricardo Probst

    2009-10-01

    Full Text Available Neste trabalho objetivou-se avaliar a sobrevivência das estacas e a produção de matéria seca na fase de cultivo de mudas das espécies forrageiras missioneira gigante (Axonopus catharinensis, amendoim forrageiro (Arachis pintoi e maku (Lotus uliginosus cv. Maku. No sistema hidropônico de leito flutuante com solução nutritiva à base de biofertilizante ou adubo solúvel. O delineamento experimental adotado foi o de blocos ao acaso em esquema fatorial 3 x 2, sendo três espécies forrageiras e duas soluções nutritivas. As espécies não apresentaram diferença quanto à sobrevivência (p = 0,225, independentemente do tipo de fertilizante (p = 0,92. No entanto, quando se quantificou a produção de MS planta-1 proporcionada por cada uma dessas espécies, o maku (p = 0,001 obteve as maiores quantidades (47,18 g, enquanto o amendoim forrageiro (19,90 g e a missioneira gigante (16,81 g foram semelhantes entre si (p = 0,227, tendo o mesmo ocorrido entre os fertilizantes (p = 0,559. Deste modo, as três espécies possuem condições semelhantes de sobrevivência, independentemente da concentração de nutrientes da solução nutritiva, com o maku proporcionando a maior produção de MS planta-1.The aim of this work was to evaluate the survival and dry matter production during the seedling culture phase of three forage species: Axonopus catharinensis, forage peanut (Arachis pintoi, and greater lotus (Lotus uliginosus cv. Maku, in a floating hydroponic system using biofertilizer or soluble fertilizer. The experimental design adopted was randomized blocks in a 3 x 2 factorial scheme, with three forage species and two fertilizers. There was no difference between species with regard to survival (p = 0.225, regardless of the fertilizer used (p = 0.92. However, when dry matter production was considered, Maku (P=0,001 showed greater weight (47.18 g, while there was no difference (p = 0.227 in weight between Arachis pintoi (19.90 g and Axonopus

  8. RENDIMENTO DO AMENDOIM-FORRAGEIRO ESTABELECIDO SOB DIFERENTES ARRANJOS POPULACIONAIS DE PLANTAS EM PLANOSSOLO

    Directory of Open Access Journals (Sweden)

    Otoniel Geter Lauz Ferreira

    2006-10-01

    Full Text Available Esta pesquisa foi realizada no Conjunto Agrotécnico Visconde da Graça, da Universidade Federal de Pelotas, RS, de dezembro/2000 a março/2002, para avaliar o estabelecimento, produção e qualidade da leguminosa forrageira Arachis pintoi (KRAPOVICKAS & GREGORY cv. Alqueire-1, em diferentes combinações de espaçamentos de plantio (0,25, 0,50, 0,75 e 1,00 m entre linhas e entre plantas, em resteva de arroz (Planossolo hidromórfico eutrófico solódico. O delineamento experimental constou de blocos casualizados com tratamentos dispostos em faixas e quatro repetições. Foram realizados três cortes, em intervalo de 45 dias, após o corte de uniformização (05/11/01, com rebaixamento a 5 cm do nível do solo. O material de cada corte foi separado em Arachis (folha e caule, gramíneas nativas, outras espécies e material morto. Foram avaliadas as produções de matéria seca e proteína bruta, teores de proteína bruta, fósforo e potássio e respectivas extrações. Foi concluído que é viável o estabelecimento, através de propagação vegetativa de A. pintoi, em áreas anteriormente cultivadas com arroz irrigado (restevas sob diferentes espaçamentos populacionais. No ano de estabelecimento produções mais elevadas de matéria seca são obtidas com populações mais densas de plantas, ou seja, distâncias menores entre linhas e entre plantas nas linhas. O espaçamento entre plantas dentro das linhas apresenta efeito mais determinante na produção de matéria seca do que a distância entre linhas. PALAVRAS-CHAVE: Arachis pintoi, espaçamento de plantio, leguminosa perene.

  9. Recovery of hillside soils, degraded by the erosion, by means of the use of biological-forest procedures

    International Nuclear Information System (INIS)

    Soil degradation is present in some areas of the Guanenta Comunero province in Andean Region of Colombia. Different responsible factors are identified: inadequate soil management (tilling in slope direction), machinery overuse and monoculture without natural cover. This carried out erosion that is severe in 40% of the affected area with furrows, gullies and barrens occurrence. For prevent the erosion were built wood barriers, established whit gramineous, leguminous and trees. The gramineous, Brachiaria decumbens was established using seeds a live material, which produced 1860, and 1631 kg/ha of dry material respectively. Arachis pintoi established like protein bank and in association reached a soil coverage of 87 % and improved disposability of Ca, Mg, K and P. farmers can easily build wooden barriers and them can retain sediments un amounts of 4.72, 23.43 and 1.50 m3 in areas of 207,494 and 129 m2 respectively

  10. Two novel aflatoxin-producing Aspergillus species from Argentinean peanuts

    DEFF Research Database (Denmark)

    Pildain, M.B.; Frisvad, Jens Christian; Vaamonde, G.; Cabral, D.; Varga, J.; Samson, R.A.

    2008-01-01

    (morphology and extrolite profiles) and molecular (beta-tubulin and calmodulin gene sequences) characters. A. minisclerotigenes resembles Aspergillus flavus and Aspergillus parvisclerotigenus in producing aflatoxins B-1 and B-2, cyclopiazonic acid, kojic acid and aspergillic acid, but in addition it produces......Two novel species from Aspergillus section Flavi from different species of Arachis (peanuts) in Argentina are described as Aspergillus arachidicola sp. nov. and Aspergillus minisclerotigenes sp. nov. Their novel taxonomic status was determined using a polyphasic taxonomic approach with phenotypic...... aflatoxins G(1) and G(2), aflavarins, aflatrem, aflavinines, parasiticolides and paspaline. This species also includes several isolates previously assigned to A. flavus group II and three Australian soil isolates. A. arachidicola produces aflatoxins B-1, B-2, G(1) and G(2), kojic acid, chrysogine and...

  11. Phylogenetic analyses of peanut resistance gene candidates and screening of different genotypes for polymorphic markers.

    Science.gov (United States)

    Radwan, Osman E; Ahmed, Talaat A; Knapp, Steven J

    2010-01-01

    The nucleotide-binding-site-leucine-rich-repeat (NBS-LRR)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. Here, we describe a collection of peanut NBS-LRR resistance gene candidates (RGCs) isolated from peanut (Arachis) species by mining Gene Bank data base. NBS-LRR sequences assembled into TIR-NBS-LRR (75.4%) and non-TIR-NBS-LRR (24.6%) subfamilies. Total of 20 distinct clades were identified and showed a high level of sequence divergence within TIR-NBS and non-TIR-NBS subfamilies. Thirty-four primer pairs were designed from these RGC sequences and used for screening different genotypes belonging to wild and cultivated peanuts. Therefore, peanut RGC identified in this study will provide useful tools for developing DNA markers and cloning the genes for resistance to different pathogens in peanut. PMID:23961057

  12. AcEST: DK953455 [AcEST

    Lifescience Database Archive (English)

    Full Text Available |B4UW61|B4UW61_ARAHY Electron transporter/thiol-disulfide exchange intermediate protein OS=Arachis...TTAKHSQGQLVPLLTEAGA 101 >sp|O81187|GLRX_VERFO Glutaredoxin OS=Vernicia fordii PE=3 SV=1 Length = 104 Score = 107 bits (266), Expect...|B4UW61|B4UW61_ARAHY Electron transporter/thiol-disulfide exch... 122 2e-26 tr|B4FRD4|B4FRD4_MAIZE G...d PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res. 25:3389-3402. Query= DK953455|Adian...aredoxin-C2 OS=Arabidopsis thaliana GN... 106 1e-22 sp|Q54GP8|GLRX_DICDI Glutaredoxin OS=Dictyostelium disco

  13. Dicty_cDB: Contig-U15470-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available str. Sg (Schizaphis graminum)... 36 0.056 18 ( AF362369 ) Dictyostelium discoideum histidine kinase DhkG (d.... ( EG346382 ) SAAH-aaa91g12.b1 Agen 0058 Schmidtea mediterranea... 40 0.22 2 ( CU570896 ) Zebrafish DNA sequ... |pid:none) Arachis duranensis microsomal olea... 67 2e-09 EU433558_1( EU433558 |pid:none) Caragana korshinskii var. intermed...3823 |pid:none) Saprolegnia diclina omega-3 fatty ... 51 9e-05 FJ907399_1( FJ9073...AC122405 ) Mus musculus BAC clone RP24-119O6 from chromosome... 38 0.15 8 ( X99669 ) Dictyostelium discoideum mRNA for dynamin

  14. Plantas de cobertura de solo como hospedeiras alternativas de Colletotrichum guaranicola Cover crops as intermediate hosts to Colletotrichum guaranicola

    Directory of Open Access Journals (Sweden)

    L.J. Mileo

    2006-12-01

    Full Text Available As plantas de cobertura de solo usadas para suprimir o crescimento de plantas daninhas podem hospedar fungos fitopatogênicos. Para testar essa hipótese, elaborou-se este trabalho com o objetivo de avaliar o comportamento de nove espécies de plantas como possíveis hospedeiras do fungo Colletotrichum guaranicola. O experimento foi conduzido em casa de vegetação sob delineamento inteiramente casualizado, com quatro repetições. Cada vaso com três plantas da mesma espécie representou uma unidade experimental. As espécies que constituíram os tratamentos foram: Arachis pintoi, Calopogonium mucunoides, Chamaecrista rotundifolia, Crotalaria striata, Desmodium ovalifolium, Flemingia congesta, Mucuna aterrima, Pueraria phaseoloides e Tephrosia candida. Quarenta dias após a semeadura, as plantas foram inoculadas com suspensão de esporos de C. guaranicola na concentração de 10(5 conídios mL¹, enquanto as plantas testemunhas receberam somente água. As plantas foram mantidas em câmara úmida por 48 horas. Diariamente, foram feitas observações por 15 dias após a inoculação, para visualizar sintomas da doença. As espécies que não apresentaram sintomas de C. guaranicola foram Arachis pintoi, Chamaecrista rotundifolia, Desmodium ovalifolium, Flemingia congesta e Tephrosia candida, e as que manifestaram sintomas após a inoculação foram Calopogonium mucunoides, Crotalaria striata, Mucuna aterrima e Pueraria phaseoloides, que podem ser fontes de inóculo do patógeno da antracnose para o guaranazeiro.Cover crops used to suppress weed growth can be intermediate hosts to phytopathogenic fungi. To test this hypothesis, nine species of cover crops were evaluated as hosts to Colletotrichum guaranicola. The experiment was arranged in a randomized design, with four replicates, and conducted under greenhouse conditions. Each vase with three plants of one species constituted one plot. The species treated were: Arachis pintoi, Calopogonium

  15. Roughage digestion evaluation in horses with total feces collection and mobile nylon bags

    Directory of Open Access Journals (Sweden)

    Liziana Maria Rodrigues

    2012-02-01

    Full Text Available This study aimed to evaluate the nutrient digestibility of roughages in horses with total feces collection and mobile bags. Two trials were carried out simultaneously. The first trial evaluated the digestibility of nutrients of coastcross hay (Cynodon dactylon cv. coastcross with total feces collection. The second trial assessed the digestibility of nutrients of alfalfa hay (Medicago sativa, peanut (Arachis pintoi and coastcross hay with mobile bags. This trial was conducted with gastric insertions of nylon bags every 12 hours, and each bag contained 663 mg of feed samples in a proportion of 17 mg DM/cm². Feces and bags were collected directly from the stall floor immediately after excretion. There was no difference between the digestibility of dry matter, crude protein, carbohydrates and hydrolysable carbohydrates of coastcross hay estimated with feces collection and mobile bags. Forage peanut showed high nutrients digestibility, with values close to those observed with alfalfa, indicating potential for use in diets for horses.

  16. Influence of drying treatments on antioxidant capacity of forage legume leaves.

    Science.gov (United States)

    Sang, Saw Yei; Jamharee, Fazrina; Prasad, K Nagendra; Azlan, Azrina; Maliki, Nurzillah

    2014-05-01

    This study was aimed to investigate the antioxidant capacities of four common forage legume leaves namely, Arachis pintoi (Pintoi), Calapogonium mucunoides (Calapo), Centrosema pubescens (Centro), and Stylosanthes guanensis (Stylo). Two different drying methods (oven-drying and freeze-drying) were employed and antioxidant activities were determined by DPPH, Ferric Reducing Antioxidant Power (FRAP) and β-carotene bleaching assays. Total phenolic content (TPC) was determined using Folin-Ciocalteu assay. Freeze-dried extract showed the highest antioxidant activities by DPPH (EC50 values 1.17-2.13 mg/ml), FRAP (147.08-246.42 μM of Fe(2+)/g), and β-carotene bleaching (57.11-78.60%) compared to oven drying. Hence, freeze drying treatment could be considered useful in retention of antioxidant activity and phenolic content. PMID:24803709

  17. Bromatologic composition of the herbaceous species of the Northeastern Brazil Caatinga Composição bromatológica de espécies herbáceas da caatinga

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    D.S. Silva

    2011-04-01

    Full Text Available The objective of this work was to evaluate the chemical composition of the pool and of four species of caatinga herbaceous vegetation in the rainy and dry seasons. The experiment was conducted in three selected shrub areas at different levels of conservation. Four samples of each species (Arachis pintoi, Boerhavia diffusa, Heliotropium ternatum, Aristida adscensionis were collected in each area and from a pool of species for determination of bromatologic composition. In the dry season, only the pool of species and the grass Aristida adscensionis were evaluated. There was a significant effect of the studied area on the chemical composition of all analyzed species. The nutrient content found in the dry matter (DM and the digestibility of the pool of species indicate that caatinga herbs presented improved quality in the rainy season. The qualitative variables of the studied species were most heterogeneous due to the variability found in caatinga. Conservation conditions in caatinga and season of the year influence bromatologic composition of the species Arachis pintoi, Boerhavia diffusa L., Heliotropium ternatum Vahl. Aristida adscensionis L. and of a pool of typical species found in Caatinga.Com o objetivo de avaliar a composição bromatológica do pool e de quatro espécies da vegetação herbácea da caatinga nos períodos chuvoso e seco foi conduzido um experimento em três áreas selecionadas de caatinga, com níveis diferenciados de conservação. Em cada área, foram colhidas quatro amostras de cada espécie (Arachis pintoi, Boerhavia diffusa, Heliotropium ternatum, Aristida adscensionis e de um pool de espécies, para determinação da composição bromatológica. Na época seca foram avaliados somente o pool de espécies e a gramínea Aristida adscensionis. Houve efeito significativo da área amostrada na composição bromatológica de todas as espécies analisadas. Os teores de nutrientes na matéria seca (MS e a digestibilidade do pool

  18. Decomposição e liberação de nutrientes de resíduos culturais de plantas de cobertura em argissolo vermelho-amarelo na região noroeste Fluminense (RJ Decomposition and nutrient release from cover crop residues in passion-fruit plantation

    Directory of Open Access Journals (Sweden)

    Antonio Carlos da Gama-Rodrigues

    2007-12-01

    Full Text Available A decomposição pode assumir importante papel no manejo da fertilidade do solo, possibilitando a elaboração de técnicas de cultivo que melhorem a utilização de nutrientes contidos nos resíduos vegetais. O objetivo deste trabalho foi estimar as taxas de decomposição e liberação de C, N, P, K, Ca e Mg de resíduos culturais provenientes de plantas de coberturas na cultura do maracujá. As espécies avaliadas foram feijão-de-porco (Canavalia ensiformis, amendoim forrageiro acesso CIAT 1734 (Arachis pintoi, siratro (Macroptilium atropurpureum, cudzu tropical (Pueraria phaseoloides e Brachiaria brizantha. A decomposição dos resíduos culturais, colocados em sacos de malha de 2 mm, foi avaliada durante 140 dias. O modelo que proporcionou melhor ajuste foi o exponencial de primeira ordem. O feijão-de-porco e o amendoim forrageiro apresentaram as maiores taxas de decomposição de matéria seca, diferindo significativamente das demais coberturas vegetais. As taxas de liberação de C, N, P, Ca e Mg foram maiores no feijão-de-porco. O amendoim forrageiro apresentou a maior taxa de liberação de K. Para todas as coberturas vegetais, os maiores valores médios de taxa de liberação foram de K e polifenóis. As taxas de liberação de C, N, P, Ca e Mg estão associadas positivamente à taxa de decomposição da matéria seca. As taxas de decomposição de matéria seca e de liberação de C, de nutrientes e de polifenóis variaram em função da qualidade nutricional e orgânica do substrato referente ao início do estudo. As distintas taxas de decomposição e liberação de nutrientes das espécies estimadas mostraram o potencial de uso de resíduos vegetais como fonte de nutrientes na cultura do maracujá.Decomposition can assume an important role in soil fertility management, underlying techniques that optimize the use of nutrients of plant residues. The objective of this study was to estimate the decomposition rate and nutrient

  19. Produção de matéria seca e controle de plantas daninhas por leguminosas consorciadas com cana-de-açúcar em cultivo orgânico

    Directory of Open Access Journals (Sweden)

    Raíssa de Araujo Dantas

    2015-08-01

    Full Text Available Resumo:O objetivo deste trabalho foi avaliar a produção de matéria seca e o controle de plantas daninhas por espécies de leguminosas consorciadas com cana-de-açúcar, em cultivo orgânico. As espécies leguminosas Arachis pintoi, Cajanus cajan, Calopogonium mucunoides, Crotalaria anagyroides, Crotalaria juncea e Stylosanthes 'Campo Grande' foram semeadas em novembro de 2012, nas entrelinhas da cana-de-açúcar. O delineamento experimental em blocos ao acaso foi utilizado com quatro repetições e dois controles - um tratamento capinado e outro sem capinar. A massa de matéria seca das leguminosas foi avaliada aos 142 dias após a semeadura (DAS, e a massa de matéria seca de plantas daninhas, aos 29, 85 e 142 DAS. A massa de matéria seca de plantas daninhas foi inferior à da testemunha em todas as épocas amostradas. A produção de massa de matéria seca das leguminosas no consórcio foi baixa. As espécies Crotalaria juncea, Cajanus cajan e Calopogonium mucunoides produziram massa de matéria seca superior à das espécies Arachis pintoi e Stylosanthes 'Campo Grande'. A produtividade de colmos da cana-de-açúcar não foi afetada pelo consórcio. O consórcio entre cana-de-açúcar orgânica e leguminosas com semeadura tardia, para adubação verde, é eficiente no controle de plantas daninhas.

  20. Influência de variáveis químicas e estruturais do dossel sobre a taxa de ingestão instantânea em bovinos manejados em pastagens tropicais Influence of structural characteristics and chemical composition of tropical grasses on the instantaneous forage intake rate

    Directory of Open Access Journals (Sweden)

    Fabíola Cristine de Almeida Rego

    2006-06-01

    Full Text Available Avaliou-se a taxa de ingestão (TI em bovinos em pastagens exclusivas de Brachiaria brizantha, Panicum maximum cv. Tanzânia, Arachis pintoi e uma consorciação de Brachiaria brizantha com Arachis pintoi com o objetivo de selecionar as características estruturais e qualitativas da planta, de maior importância para estimativa da taxa de ingestão dos animais. Os animais pastejavam em pares, passando por todas as alturas e espécies em dias sucessivos. Após jejum de três horas, permaneciam por 60 minutos na área experimental, onde foram quantificados o tempo efetivo de pastejo e o número de bocados. A quantidade de forragem ingerida foi estimada pela técnica de dupla pesagem. As características estruturais da pastagem utilizadas na equação para determinar a TI foram altura média da pastagem (cm, proporção dos componentes morfológicos (%, massa dos componentes morfológicos (t MS/ha e densidade de matéria seca dos componentes morfológicos (kg MS/ha/cm. As características qualitativas foram expressas em termos de PB e de FDN. As variáveis da pastagem foram selecionadas pelo procedimento estatístico stepwise. As equações das TI, definidas a partir das características estudadas, foram: capim-marandu: TI = 59,8980 + 0,7299 LV + 3,5777 MF - 1,2459 FDNL + 0,2882 ALT (LV - proporção de lâminas verdes, MF - massa de forragem, FDNL - FDN de lâminas, ALT - altura média da pastagem. Capim-tanzânia: TI = 111,762 - 4,1532 PBL + 0,3469 LV - 0,5207 FDNL (PB de lâminas, LV - proporção de lâminas verdes, FDNL - FDN de lâminas. Amendoim forrageiro: TI = -196,589 + 12,1978 PBH + 8,3406 MF + 1,1060 HV + 17,3669 MLV (PBH - PB de HASTEs, MF - massa de forragem, DLV - massa de lâminas verdes. Consorciação: TI = -7,25 + 1,15ALTA - 0,22ALTI + 18,49MA - 9,88MLM + 0,49ALTM + 1,00PBL (ALTA - altura amendoim forrageiro, ALTI - altura de invasoras, MA - massa de amendoim forrageiro, MLM - massa de lâminas verdes de capim-marandu, ALTM