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Sample records for aqueous two-phase system

  1. Aqueous two-phase system based on natural quaternary ammonium compounds for the extraction of proteins.

    Science.gov (United States)

    Zeng, Chao-Xi; Xin, Rui-Pu; Qi, Sui-Jian; Yang, Bo; Wang, Yong-Hua

    2016-02-01

    Aqueous two-phase systems, based on the use of natural quaternary ammonium compounds, were developed to establish a benign biotechnological route for efficient protein separation. In this study, aqueous two-phase systems of two natural resources betaine and choline with polyethyleneglycol (PEG400/600) or inorganic salts (K2 HPO4 /K3 PO4 ) were formed. It was shown that in the K2 HPO4 -containing aqueous two-phase system, hydrophobic interactions were an important driving force of protein partitioning, while protein size played a vital role in aqueous two-phase systems that contained polyethylene glycol. An extraction efficiency of more than 90% for bovine serum albumin in the betaine/K2 HPO4 aqueous two-phase system can be obtained, and this betaine-based aqueous two-phase system provided a gentle and stable environment for the protein. In addition, after investigation of the cluster phenomenon in the betaine/K2 HPO4 aqueous two-phase systems, it was suggested that this phenomenon also played a significant role for protein extraction in this system. The development of aqueous two-phase systems based on natural quaternary ammonium compounds not only provided an effective and greener method of aqueous two-phase system to meet the requirements of green chemistry but also may help to solve the mystery of the compartmentalization of biomolecules in cells.

  2. Two-phase aqueous micellar systems: an alternative method for protein purification

    Directory of Open Access Journals (Sweden)

    Rangel-Yagui C. O.

    2004-01-01

    Full Text Available Two-phase aqueous micellar systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental and theoretical work by Blankschtein and co-workers on the use of two-phase aqueous micellar systems for the separation of hydrophilic proteins. The experimental partitioning behavior of the enzyme glucose-6-phosphate dehydrogenase (G6PD in two-phase aqueous micellar systems is also reviewed and new results are presented. Specifically, we discuss very recent work on the purification of G6PD using: i a two-phase aqueous micellar system composed of the nonionic surfactant n-decyl tetra(ethylene oxide (C10E4, and (ii a two-phase aqueous mixed micellar system composed of C10E4 and the cationic surfactant decyltrimethylammonium bromide (C10TAB. Our results indicate that the two-phase aqueous mixed (C10E4/C10TAB micellar system can improve significantly the partitioning behavior of G6PD relative to that observed in the two-phase aqueous C10E4 micellar system.

  3. Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system

    OpenAIRE

    Ilić Sanja M.; Đaković Sanja D.; Cvejić Jelena H.; Antov Mirjana G.; Zeković Zoran P.

    2005-01-01

    The study is concerned with the conversion of apigenin-7-O-β-glucoside into apigenin in polyethylene glycol 6000 / dextran 20000 aqueous two-phase system by β-glucosidase. Apigenin was separated from apigenin-7-O-β-glucoside and β-glucosidase by their partition into opposite phases. In 14% PEG / 22.5% DEX aqueous two-phase system obtained yield of apigenin in top phase was 108%.

  4. Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system

    Directory of Open Access Journals (Sweden)

    Ilić Sanja M.

    2005-01-01

    Full Text Available The study is concerned with the conversion of apigenin-7-O-β-glucoside into apigenin in polyethylene glycol 6000 / dextran 20000 aqueous two-phase system by β-glucosidase. Apigenin was separated from apigenin-7-O-β-glucoside and β-glucosidase by their partition into opposite phases. In 14% PEG / 22.5% DEX aqueous two-phase system obtained yield of apigenin in top phase was 108%.

  5. The Effect of pH Difference Between Two Phases on the Partition of Lysozyme in Aqueous Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    In the investigation of effect of KSCN on the partitioning of lysozyme in PEG2000/ammonium sulfate aqueous two-phase system, it was found that the KSCN could alter the pH difference between the two phases, and thus affect the partition of lysozyme. The relationship between partition coefficients of lysozyme and pH differences between two phases was discussed.

  6. Affinity partitioning of human antibodies in aqueous two-phase systems

    NARCIS (Netherlands)

    Rosa, P. A. J.; Azevedo, A. M.; Ferreira, I. F.; de Vries, J.; Korporaal, R.; Verhoef, H. J.; Visser, T. J.; Aires-Barros, M. R.

    2007-01-01

    The partitioning of human immunoglobulin (IgG) in a polymer-polymer and polymer-salt aqueous two-phase system (ATPS) in the presence of several functionalised polyethylene glycols (PEGs) was studied. As a first approach, the partition studies were performed with pure IgG using systems in which the t

  7. Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems

    NARCIS (Netherlands)

    Rodenbrock, A.; Selber, K.; Egmond, M.R.; Kula, M.-R.

    2010-01-01

    Detergent-based aqueous two-phase systems have the advantage to require only one auxiliary chemical to induce phase separation above the cloud point. In a systematic study the efficiency of tryptophan-rich peptide tags was investigated to enhance the partitioning of an enzyme to the detergent-rich p

  8. Selective separation and enrichment of proteins in aqueous two-phase extraction system

    Institute of Scientific and Technical Information of China (English)

    Feng Qu; Hao Qin; Min Dong; Dong Xu Zhao; Xin Ying Zhao; Jing Hua Zhang

    2009-01-01

    A simple aqueous two-phase extraction system(ATPS)of PEG/phosphate was proposed for selective separation and enrichment of proteins.The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases.Five proteins (bovine serum albumin,Cytochrome C,lysozyme,myoglobin,and trypsin)were used as model proteins to study the effect of phosphate concentration and pH on proteins partition.The PEG/phosphate system was firstly applied to real human saliva and plasma samples,some proteins showed obviously different partition in two phases.The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.

  9. Drop volumes and terminal velocities in aqueous two-phase systems

    Energy Technology Data Exchange (ETDEWEB)

    Bhavasar, P. M.; Jafarabad, K. R.; Pandit, A. B.; Sawant, S. B.; Joshi, J. B. [Bombay Univ. (India). Dept. of Chemical Technology

    1996-12-01

    Two phase aqueous extraction techniques employed in liquid-liquid extraction equipment such as spray columns and plate columns were studied, with particular attention to predicting drop sizes prior to jetting, and their terminal velocity. In the particular system studied, the values obtained by conventional models as found in the literature were considered inapplicable. A generalised model was constructed using video photographic measurements, and a correlation was developed for the terminal velocities of the drops in aqueous two-phase systems. This simplified model was found to be successful in expressing the terminal rise/fall velocities of droplets covering a specific range of Morton numbers (representing physical properties) from 0.00211 to 11050 and Eotvos numbers (representative of drop size) from 0.091 to 288. 22 refs., 6 figs.

  10. Preparative crystallization of a single chain antibody using an aqueous two-phase system.

    Science.gov (United States)

    Huettmann, Hauke; Berkemeyer, Matthias; Buchinger, Wolfgang; Jungbauer, Alois

    2014-11-01

    A simultaneous crystallization and aqueous two-phase extraction of a single chain antibody was developed, demonstrating process integration. The process conditions were designed to form an aqueous two-phase system, and to favor crystallization, using sodium sulfate and PEG-2000. At sufficiently high concentrations of PEG, a second phase was generated in which the protein crystallization occurred simultaneously. The single chain antibody crystals were partitioned to the top, polyethylene glycol-rich phase. The crystal nucleation took place in the sodium sulfate-rich phase and at the phase boundary, whereas crystal growth was progressing mainly in the polyethylene glycol-rich phase. The crystals in the polyethylene glycol-rich phase grew to a size of >50 µm. Additionally, polyethylene glycol acted as an anti-solvent, thus, it influenced the crystallization yield. A phase diagram with an undersaturation zone, crystallization area, and amorphous precipitation zone was established. Only small differences in polyethylene glycol concentration caused significant shifts of the crystallization yield. An increase of the polyethylene glycol content from 2% (w/v) to 4% (w/v) increased the yield from approximately 63-87%, respectively. Our results show that crystallization in aqueous two-phase systems is an opportunity to foster process integration.

  11. Aqueous Two-Phase Systems formed by Biocompatible and Biodegradable Polysaccharides and Acetonitrile.

    Science.gov (United States)

    de Brito Cardoso, Gustavo; Souza, Isabela Nascimento; Pereira, Matheus M; Freire, Mara G; Soares, Cleide Mara Faria; Lima, Álvaro Silva

    2014-11-05

    In this work, it is shown that novel aqueous two-phase systems can be formed by the combination of acetonitrile and polysaccharides, namely dextran. Several ternary phase diagrams were determined at 25 °C for the systems composed of water + acetonitrile + dextran. The effect of the dextran molecular weight (6,000, 40,000 and 100,000 g.mol(-1)) was ascertained toward their ability to undergo liquid-liquid demixing. An increase in the dextran molecular weight favors the phase separation. Furthermore, the effect of temperature (25, 35 and 45 °C) was evaluated for the system constituted by the dextran of higher molecular weight. Lower temperatures are favorable for phase separation since lower amounts of dextran and acetonitrile are required for the creation of aqueous two-phase systems. In general, acetonitrile is enriched in the top phase while dextran is majorly concentrated in the bottom phase. The applicability of this new type of two-phase systems as liquid-liquid extraction approaches was also evaluated by the study of the partition behavior of a well-known antioxidant - vanillin - and used here as a model biomolecule. The optimized conditions led to an extraction efficiency of vanillin of 95% at the acetonitrile-rich phase.

  12. Correlations for the partition behavior of proteins in aqueous two-phase systems

    DEFF Research Database (Denmark)

    Schmidt, A.S.; Andrews, B.A.; Asenjo, J.A.

    1996-01-01

    was lower than that of subtilisin which was probably due to its higher hydrophobicity and, hence, a stronger salting-out effect. The protein concentration in each of the two phases was correlated with a ''saturation''-type equation. The partition coefficient could be satisfactorily predicted, as a function...... of the overall protein concentration, by the ratio between the ''saturation'' equations of the two individual phases. Better correlations were obtained when an empirical sigmoidal Boltzmann equation was fitted to the data, since in virtually all cases the partition coefficient is constant at low protein......The effect of protein concentration in partitioning in PEG/ salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system...

  13. Interfacial Tension Effect on Cell Partition in Aqueous Two-Phase Systems.

    Science.gov (United States)

    Atefi, Ehsan; Joshi, Ramila; Mann, Jay Adin; Tavana, Hossein

    2015-09-30

    Aqueous two-phase systems (ATPS) provide a mild environment for the partition and separation of cells. We report a combined experimental and theoretical study on the effect of interfacial tension of polymeric ATPS on the partitioning of cells between two phases and their interface. Two-phase systems are generated using polyethylene glycol and dextran of specific properties as phase-forming polymers and culture media as the solvent component. Ultralow interfacial tensions of the solutions are precisely measured using an axisymmetric drop shape analysis method. Partition experiments show that two-phase systems with an interfacial tension of 30 μJ/m(2) result in distribution of majority of cells to the bottom dextran phase. An increase in the interfacial tension results in a distribution of cells toward the interface. An independent cancer cell spheroid formation assay confirms these observations: a drop of the dextran phase containing cancer cells is dispensed into the immersion polyethylene glycol phase to form a cell-containing drop. Only at very small interfacial tensions do cells remain within the drop to aggregate into a spheroid. We perform a thermodynamic modeling of cell partition to determine variations of free energy associated with displacement of cells in ATPS with respect to the ultralow interfacial tensions. This modeling corroborates with the experimental results and demonstrates that at the smallest interfacial tension of 30 μJ/m(2), the free energy is a minimum with cells in the bottom phase. Increasing the interfacial tension shifts the minimum energy and partition of cells toward the interfacial region of the two aqueous phases. Examining differences in the partition behavior and minimum free energy modeling of A431.H9 cancer cells and mouse embryonic stem cells shows that the surface properties of cells further modulate partition in ATPS. This combined approach provides a fundamental understanding of interfacial tension role on cell partition in

  14. Integration of bioconversion and downstream processing: starch hydrolysis in an aqueous two-phase system.

    Science.gov (United States)

    Larsson, M; Arasaratnam, V; Mattiasson, B

    1989-02-05

    Integration of bioconversion and the first step(s) of down stream processing can be used as a means to increase the productivity of bioprocesses. This integration also gives the possibility to run the bioconversion in a continuous mode. We demonstrate the use of an aqueous two-phase system in combination with ultrafiltration to accomplish this. Conversion of native starch to glucose by alpha-amylase and glucoamylase was carried out in an aqueous two-phase system in connection with a membrane filtration unit. In this way, a continuous stream of glucose in buffer solution was obtained; the phase-forming polymers as well as the starch-degrading enzymes were recycled, and clogging of the ultrafiltration membrane was avoided. The process was carried out continuously in a mixer-settler reactor for a period of 8 days. The enzyme activities in the top and bottom phases and in the mixing chamber were monitored intermittently throughout the experiment. The optimum pH, temperature, and ionic strength for the activity of the enzyme mixture were determined. The settling time of phase systems containing varying amounts of PEG, crude dextran, and solid starch was studied. The activity and stability of enzyme mixtures was studied both in buffer medium and in the medium containing the polymers. The enzymes were found to be more active and stable in medium containing polymers than in the buffer solutions.

  15. Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets

    Science.gov (United States)

    Jia, Tony Z.; Hentrich, Christian; Szostak, Jack W.

    2014-02-01

    Compartmentalization in a prebiotic setting is an important aspect of early cell formation and is crucial for the development of an artificial protocell system that effectively couples genotype and phenotype. Aqueous two-phase systems (ATPSs) and complex coacervates are phase separation phenomena that lead to the selective partitioning of biomolecules and have recently been proposed as membrane-free protocell models. We show in this study through fluorescence recovery after photobleaching (FRAP) microscopy that despite the ability of such systems to effectively concentrate RNA, there is a high rate of RNA exchange between phases in dextran/polyethylene glycol ATPS and ATP/poly-L-lysine coacervate droplets. In contrast to fatty acid vesicles, these systems would not allow effective segregation and consequent evolution of RNA, thus rendering these systems ineffective as model protocells.

  16. Selective separation of protein and saccharides by ionic liquids aqueous two-phase systems

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    In the present work,it was found that aqueous solution of a hydrophilic ionic liquid (IL),1-butyl-3-methylimidazolium dicyanamide ([C4mim][N(CN)2]),could be separated into an aqueous two-phase system (ATPS) by inorganic salts such as K2HPO4 and K3PO4.The top phase is IL-rich,while the bottom phase is phosphate-rich.It was shown that 82.7%-100% bovine serum albumin (BSA) could be enriched into the top phase and almost quantitative saccharides (arabinose,glucose,sucrose,raffinose or dextran) were preferentially extracted into the bottom phase in a single-step extraction by [C4mim][N(CN)2] + K2HPO4 ATPS.The extraction efficiency of BSA from the aqueous saccharide solutions was influenced by the molecular structure of saccharides.The conductivity,dynamic light scattering (DLS) and transmission electron microscopy (TEM) were combined to investigate the microstructure of the IL-rich top phase and the possible mechanism for the selective separation.It is suggested that the formation of the IL aggregate and the IL aggregate-BSA complex plays a significant role in the separation of BSA from aqueous saccharide solutions.This is the first example for the selective separation by ILs-based ATPSs.It is expected that these findings would have potential applications in bio-analysis,separation,and IL recycle.

  17. Prostaglandin H synthase kinetics in the two-phase aqueous-micellar system.

    Science.gov (United States)

    Ponomareva, Olga A; Trushkin, Nikita A; Filimonov, Ivan S; Krivoshey, Alexandr V; Barkhatov, Vladimir I; Mitrofanov, Sergey I; Vrzheshch, Petr V

    2016-09-01

    Reaction mixture for PGHS (prostaglandin-H-synthase) is a two-phase system including micellar hydrophobic phase and hydrophilic aqueous phase. Reagents added to the mixture are distributed between phases, thus concentrations of reagents dissolved in phases can differ significantly from their overall contents. Using dynamic light scattering we found that the hydrophobic phase produced by tween-20 consists of micelles, which radius (4-5nm) does not depend on either tween-20 overall content (0.1%-1% v/v) or arachidonic acid (AA) addition (10-1000μM) or PGHS addition (1μM). Tween-20 overall content changing from 0.1% to 2% v/v dramatically affected COX kinetic, but accounting AA distribution between phases allowed us to estimate "true" parameters, independent of the tween-20 overall content and the concentration of another substrate: KM(Ox) equals 9.8μM O2 in the aqueous phase or 0.0074bar in the gaseous phase, KM(AA) equals 5400μM AA in the phase of tween-20 micelles and 5400/PμM AA in the aqueous phase (P is the distribution ratio for the AA between the aqueous phase and the hydrophobic phase (P≫1000)). This approach allowed to evaluate PS, the distribution ratio for the AA between the hydrophobic phase and the PGHS active center (PS ~310). This coefficient indicates the AA selectivity toward the cyclooxygenase active center.

  18. Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system

    OpenAIRE

    Carneiro-da-Cunha, M. N.; Souza, K. P. S.; Mota, A; J.A. Teixeira; Porto, C S; Porto, Tatiana Souza; Porto, Ana L. F.

    2014-01-01

    β-Lactamases are enzymes responsible for the hydrolysis of β-lactam antibiotics, being produced by several pathogenic bacteria. Clavulanic acid is a commercially and clinically important β-lactamase inhibitor, its extraction being possible by the application of aqueous two-phase system. In this study, clavulanic acid stability was investigated at different molar mass PEG (400, 1 000 and 20 000 g mol−1) and at different citrate concentrations (5 and 20%) PEG/citrate aqueous-two phase systems (...

  19. Ultralow interfacial tensions of aqueous two-phase systems measured using drop shape.

    Science.gov (United States)

    Atefi, Ehsan; Mann, J Adin; Tavana, Hossein

    2014-08-19

    Aqueous solutions of different polymers can separate and form aqueous two-phase systems (ATPS). ATPS provide an aqueous, biocompatible, and mild environment for separation and fractionation of biomolecules. The interfacial tension between the two aqueous phases plays a major role in ATPS-mediated partition of biomolecules. Because of the structure of the two aqueous phases, the interfacial tensions between the phases can be 3-4 orders of magnitude smaller than conventional fluid-liquid systems: ∼1-100 μJ/m(2) for ATPS compared to ∼72 mJ/m(2) for the water-vapor interface. This poses a major challenge for the experimental measurements of reproducible interfacial tension data for these systems. We address the need for precise determination of ultralow interfacial tensions by systematically studying a series of polymeric ATPS comprising of polyethylene glycol (PEG) and dextran (DEX) as the phase-forming polymers. Sessile and pendant drops of the denser DEX phase are formed within the immersion PEG phase. An axisymmetric drop shape analysis (ADSA) is used to determine interfacial tensions of eight different ATPS. Specific criteria are used to reproducibly determine ultralow interfacial tensions of the ATPS from pendant and sessile drops. Importantly, for a given ATPS, pendant drop and sessile drop experiments return values within 0.001 mJ/m(2) indicating reliability of our measurements. Then, the pendant drop technique is used to measure interfacial tensions of all eight ATPS. Our measured values range from 0.012 ± 0.001 mJ/m(2) to 0.381 ± 0.006 mJ/m(2) and vary with the concentration of polymers in equilibrated phases of ATPS. Measurements of ultralow interfacial tensions with such reproducibility will broadly benefit studies involving partition of different biomolecules in ATPS and elucidate the critical effect of interfacial tension.

  20. Bromelain purification through unconventional aqueous two-phase system (PEG/ammonium sulphate).

    Science.gov (United States)

    Coelho, D F; Silveira, E; Pessoa Junior, A; Tambourgi, E B

    2013-02-01

    This paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. The main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. The analysis of the results was based on the response surface methodology and revealed that the use of the desirability optimisation methodology (DOM) was necessary to achieve higher purification factor values and greater bromelain recovery. The use of DOM yielded an 11.80-fold purification factor and 66.38 % biological activity recovery using poly(ethylene glycol) (PEG) with a molar mass of 4,000, 10.86 % PEG concentration (m/m) and 36.21 % saturation of ammonium sulphate.

  1. Ionic Liquid-salt Aqueous Two-phase System, a Novel System for the Extraction of Abused Drugs

    Institute of Scientific and Technical Information of China (English)

    She Hong LI; Chi Yang HE; Hu Wei LIU; Ke An LI; Feng LIU

    2005-01-01

    A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated systematically. A satisfactory extraction efficiency of 93%was obtained for papaverine while that of morphine was 65%. The extraction mechanism was primarily discussed.

  2. A green deep eutectic solvent-based aqueous two-phase system for protein extracting

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Kaijia; Wang, Yuzhi, E-mail: wyzss@hnu.edu.cn; Huang, Yanhua; Li, Na; Wen, Qian

    2015-03-15

    Highlights: • A strategy for the protein purification with a deep eutectic solvent(DES)-based aqueous two-phase system. • Choline chloride-glycerin DES was selected as the extraction solvent. • Bovine serum albumin and trypsin were used as the analytes. • Aggregation phenomenon was detected in the mechanism research. - Abstract: As a new type of green solvent, deep eutectic solvent (DES) has been applied for the extraction of proteins with an aqueous two-phase system (ATPS) in this work. Four kinds of choline chloride (ChCl)-based DESs were synthesized to extract bovine serum albumin (BSA), and ChCl-glycerol was selected as the suitable extraction solvent. Single factor experiments have been done to investigate the effects of the extraction process, including the amount of DES, the concentration of salt, the mass of protein, the shaking time, the temperature and PH value. Experimental results show 98.16% of the BSA could be extracted into the DES-rich phase in a single-step extraction under the optimized conditions. A high extraction efficiency of 94.36% was achieved, while the conditions were applied to the extraction of trypsin (Try). Precision, repeatability and stability experiments were studied and the relative standard deviations (RSD) of the extraction efficiency were 0.4246% (n = 3), 1.6057% (n = 3) and 1.6132% (n = 3), respectively. Conformation of BSA was not changed during the extraction process according to the investigation of UV–vis spectra, FT-IR spectra and CD spectra of BSA. The conductivity, dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to explore the mechanism of the extraction. It turned out that the formation of DES–protein aggregates play a significant role in the separation process. All the results suggest that ChCl-based DES-ATPS are supposed to have the potential to provide new possibilities in the separation of proteins.

  3. Development of tropine-salt aqueous two-phase systems and removal of hydrophilic ionic liquids from aqueous solution.

    Science.gov (United States)

    Wu, Haoran; Yao, Shun; Qian, Guofei; Song, Hang

    2016-08-26

    A novel aqueous two-phase systems (ATPS) composed of a small molecule organic compound tropine and an organic or inorganic salt aqueous solution has been developed for the first time. The phase behavior of tropine-salt ATPS was systemically investigated and the phase equilibrium data were measured in different temperatures and concentrations and correlated by the Merchuk equation with satisfactory results. The detection of the conductivity and particle size proved the formation of micelle in the process of forming tropine-salt ATPS. The separation application of the ATPS was assessed with the removal of hydrophilic benzothiazolium-based ionic liquids (ILs) from aqueous solution. The result showed that ILs were effectively extracted into the top tropine-rich phase. Finally, ILs in the top tropine-rich phase were further separated by the means of adsorption-desorption with DM301 macroporous resin and ethanol. The method of novel tropine-salt ATPS combined with adsorption-desorption is demonstrated a promising alternative thought and approach for the removal or recovery of hydrophilic compounds from aqueous media and also could provide a potential application for bio-separation.

  4. Study of organic compounds-water interactions by partition in aqueous two-phase systems.

    Science.gov (United States)

    Madeira, Pedro P; Bessa, Ana; Teixeira, Miguel A; Álvares-Ribeiro, Luís; Aires-Barros, M Raquel; Rodrigues, Alírio E; Zaslavsky, Boris Y

    2013-12-27

    Partition coefficients of fourteen organic compounds were determined in 10 or 20 different polymer/polymer aqueous two-phase systems (ATPS) all at physiological pH (0.15M NaCl in 0.01M phosphate buffer, pH 7.4). Solute-specific coefficients characterizing different types of solute-water interactions for the compounds examined were determined by the multiple linear regression analysis. It is shown that (i) the partition behavior for the polar organic compounds is affected not only by dipole-dipole and hydrogen-bond interactions with aqueous environment but, notably, in most cases also by dipole-ion interactions; (ii) it is possible to predict partition behavior for compounds with pre-determined solute-specific coefficients in ATPS with characterized solvent features; and (iii) linear combinations of the solute-specific coefficients for the organic compounds might be useful in the development of quantitative structure-activity relationship (QSAR) analysis to describe their odor detection threshold.

  5. Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system

    Institute of Scientific and Technical Information of China (English)

    XU Ying; HE Guo-qing; LI Jing-jun

    2005-01-01

    This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems was investigated. The suitable system for elastase extraction was PEG/KH2PO4-K2HPO4, in which elastase is mainly partitioned into the PEG-rich phase,while the cells remained in the other phase. The influence of defined system parameters (e.g. PEG molecular mass, pH, NaCl addition) on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KH2PO4-K2HPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% (w/w) PEG 2 000 and 11.7% (w/w) KH2PO4-K2HPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction.

  6. Molecular features determining different partitioning patterns of papain and bromelain in aqueous two-phase systems.

    Science.gov (United States)

    Rocha, Maria Victoria; Nerli, Bibiana Beatriz

    2013-10-01

    The partitioning patterns of papain (PAP) and bromelain (BR), two well-known cysteine-proteases, in polyethyleneglycol/sodium citrate aqueous two-phase systems (ATPSs) were determined. Polyethyleneglycols of different molecular weight (600, 1000, 2000, 4600 and 8000) were assayed. Thermodynamic characterization of partitioning process, spectroscopy measurements and computational calculations of protein surface properties were also carried out in order to explain their differential partitioning behavior. PAP was observed to be displaced to the salt-enriched phase in all the assayed systems with partition coefficients (KpPAP) values between 0.2 and 0.9, while BR exhibited a high affinity for the polymer phase in systems formed by PEGs of low molecular weight (600 and 1000) with partition coefficients (KpBR) values close to 3. KpBR values resulted higher than KpPAP in all the cases. This difference could be assigned neither to the charge nor to the size of the partitioned biomolecules since PAP and BR possess similar molecular weight (23,000) and isoelectric point (9.60). The presence of highly exposed tryptophans and positively charged residues (Lys, Arg and His) in BR molecule would be responsible for a charge transfer interaction between PEG and the protein and, therefore, the uneven distribution of BR in these systems.

  7. LPS-protein aggregation influences protein partitioning in aqueous two-phase micellar systems.

    Science.gov (United States)

    Lopes, André Moreni; Santos-Ebinuma, Valéria de Carvalho; Novaes, Leticia Celia de Lencastre; Molino, João Vitor Dutra; Barbosa, Leandro Ramos Souza; Pessoa, Adalberto; Rangel-Yagui, Carlota de Oliveira

    2013-07-01

    Lipopolysaccharide endotoxins (LPS) are the most common pyrogenic substances in recombinant peptides and proteins purified from Gram-negative bacteria, such as Escherichia coli. In this respect, aqueous two-phase micellar systems (ATPMS) have already proven to be a good strategy to purify recombinant proteins of pharmaceutical interest and remove high LPS concentrations. In this paper, we review our recent experimental work in protein partitioning in Triton X-114 ATPMS altogether with some new results and show that LPS-protein aggregation can influence both protein and LPS partitioning. Green fluorescent protein (GFPuv) was employed as a model protein. The ATPMS technology proved to be effective for high loads of LPS removal into the micelle-rich phase (%REM(LPS) > 98 %) while GFPuv partitioned preferentially to the micelle-poor phase (K GFP(uv) system. Nonetheless, ATPMS can still be considered as an efficient strategy for high loads of LPS removal, but being aware that the excluded-volume partitioning theory available might overestimate partition coefficient values due to the presence of protein-LPS aggregation.

  8. Purification and in situ immobilization of papain with aqueous two-phase system.

    Science.gov (United States)

    Li, Mingliang; Su, Erzheng; You, Pengyong; Gong, Xiangyu; Sun, Ming; Xu, Diansheng; Wei, Dongzhi

    2010-12-13

    Papain was purified from spray-dried Carica papaya latex using aqueous two-phase system (ATPS). Then it was recovered from PEG phase by in situ immobilization or preparing cross-linked enzyme aggregates (CLEAs). The Plackett-Burman design and the central composite design (CCD) together with the response surface methodology (RSM) were used to optimize the APTS processes. The highly purified papain (96-100%) was achieved under the optimized conditions: 40% (w/w) 15 mg/ml enzyme solution, 14.33-17.65% (w/w) PEG 6000, 14.27-14.42% (w/w) NaH2PO4/K2HPO4 and pH 5.77-6.30 at 20°C. An in situ enzyme immobilization approach, carried out by directly dispersing aminated supports and chitosan beads into the PEG phase, was investigated to recover papain, in which a high immobilization yield (>90%) and activity recovery (>40%) was obtained. Moreover, CLEAs were successfully used in recovering papain from PEG phase with a hydrolytic activity hundreds times higher than the carrier-bound immobilized papain.

  9. Aqueous Two-phase Systems with Ultrasonic Extraction Used for Extracting Phenolic Compounds from Inonotus obliquus

    Institute of Scientific and Technical Information of China (English)

    ZHAO; Yan-xia; LIU; Yu-bing; LIU; Feng; ZHENG; Wei-fa

    2013-01-01

    Objective To optimize the extracting technology of assessing the maximum yield of phenolic compounds (PC) from Inonotus obliquus by single factor experiments and orthogonal array design methods through aqueous two-phase systems combined with ultrasonic extraction. Methods The range of the independent variables, namely levels of acetone and ammonium sulfate, and ultrasonic time were identified by a first set of single factor experiments. The actual values of the independent variables coded at four levels and three factors were selected based on the results of the single factor experiments. Subsequently, the levels of acetone and ammonium sulfate, and ultrasonic time were optimized using the orthogonal array method. Results The optimum conditions for the extraction of PC were found to use 7.0 mL acetone, 5.5 mg ammonium sulfate, with ultrasonic time for 5 min. Under these optimized conditions, the experimental maximum yield of PC was 37.8 mg/g, much higher than that of the traditional ultrasonic extraction (UE, 29.0 mg/g). And the PC obtained by this method had stronger anti-oxidative activities than those by traditional UE method. Conclusion These results indicate the suitability of the models developed and the success in optimizing the extraction conditions. This is an economical and efficient method for extracting polyphenols from I. obliquus.

  10. Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans

    Directory of Open Access Journals (Sweden)

    Tao Guo

    2015-08-01

    Full Text Available In the study, an aqueous two phase system (ATPS coupled with ultrasound was employed to extract lignans from Zanthoxylum armatum. Three standard lignans, namely (−-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM and artificial neural network (ANN. The optimal condition for ATPS with 20% n-propanol and 24% (NH42SO4 coupled with ultrasonic-assisted extraction including a solvent to solid ratio of 15:1, a temperature of 40 °C, and a treatment time of 55 min was obtained. Under the condition, the yield of (−-fargesin increased 15.12%, and the purities of (−-fargesin, sesamin and L-asarinin reached 2.222%, 1.066%, and 1.583%, with an increase of 44.38%, 25.70%, and 26.34% compared to those extracted with 95% ethanol, respectively. Coefficient of the determined (0.9855 and mean squared error (0.0018 of ANN model suggested good fitness and generalization of the ANN. Taken together, the results showed that ultrasonic-assisted ATPS can be a suitable method for extraction and enrichment of lignans from Z. armatum.

  11. Aqueous Two-phase Systems with Ultrasonic Extraction Used for Extracting Phenolic Compounds from Inonotus obliquus

    Institute of Scientific and Technical Information of China (English)

    ZHAO Yan-xia; LIU Yu-bing; LIU Feng; ZHENG Wei-fa

    2013-01-01

    Objective To optimize the extracting technology of assessing the maximum yield of phenolic compounds (PC)from Inonotus obliquus by single factor experiments and orthogonal array design methods through aqueous two-phase systems combined with ultrasonic extraction.Methods The range of the independent variables,namely levels of acetone and ammonium sulfate,and ultrasonic time were identified by a first set of single factor experiments.The actual values of the independent variables coded at four levels and three factors were selected based on the results of the single factor experiments.Subsequently,the levels of acetone and ammonium sulfate,and ultrasonic time were optimized using the orthogonal array method.Results The optimum conditions for the extraction of PC were found to use 7.0 mL acetone,5.5 mg ammonium sulfate,with ultrasonic time for 5 min.Under these optimized conditions,the experimental maximum yield of PC was 37.8 mg/g,much higher than that of the traditional ultrasonic extraction (UE,29.0 mg/g).And the PC obtained by this method had stronger anti-oxidative activities than those by traditional UE method.Conclusion These results indicate the suitability of the models developed and the success in optimizing the extraction conditions.This is an economical and efficient method for extracting polyphenols from Ⅰ.obliquus.

  12. Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures.

    Science.gov (United States)

    Moon, Byeong-Ui; Jones, Steven G; Hwang, Dae Kun; Tsai, Scott S H

    2015-06-07

    We present a technique that generates droplets using ultralow interfacial tension aqueous two-phase systems (ATPS). Our method combines a classical microfluidic flow focusing geometry with precisely controlled pulsating inlet pressure, to form monodisperse ATPS droplets. The dextran (DEX) disperse phase enters through the central inlet with variable on-off pressure cycles controlled by a pneumatic solenoid valve. The continuous phase polyethylene glycol (PEG) solution enters the flow focusing junction through the cross channels at a fixed flow rate. The on-off cycles of the applied pressure, combined with the fixed flow rate cross flow, make it possible for the ATPS jet to break up into droplets. We observe different droplet formation regimes with changes in the applied pressure magnitude and timing, and the continuous phase flow rate. We also develop a scaling model to predict the size of the generated droplets, and the experimental results show a good quantitative agreement with our scaling model. Additionally, we demonstrate the potential for scaling-up of the droplet production rate, with a simultaneous two-droplet generating geometry. We anticipate that this simple and precise approach to making ATPS droplets will find utility in biological applications where the all-biocompatibility of ATPS is desirable.

  13. Aqueous two phase system based on ionic liquid for isolation of quinine from human plasma sample.

    Science.gov (United States)

    Flieger, J; Czajkowska-Żelazko, A

    2015-01-01

    Aqueous two phase system was applied for selective extraction of quinine from human plasma. Bi-phase was constructed from ionic liquid: butyl-methyl-imidazolium chloride after addition kosmotropic salts K₃PO₄ or KH₂PO₄. Quinine was determined in plasma samples after drinking of tonic containing quinine. Determination was performed by HPLC on 5-μm Zorbax SB-CN column and eluent containing 40% acetonitrile (v/v), 20 mM phosphate buffer at pH 3 and 40 mM NaPF₆ using external standard method. The spectrophotometric detection was set λ=214 nm. Selective fluorescence detection was performed at excitation of 325 nm and emission of 375 nm. Proposed strategy provides suitable sample purification and gives extraction yields in the range of 89-106%. The determination coefficient (R(2)) has a value ≥0.997 in the range of 50-800 ng/ml quinine concentration. The limit of quantification was set at 27.9 ng/ml and the detection limit was found to be 8.4 ng/ml under fluorescence detection.

  14. Extraction of proteins with ionic liquid aqueous two-phase system based on guanidine ionic liquid.

    Science.gov (United States)

    Zeng, Qun; Wang, Yuzhi; Li, Na; Huang, Xiu; Ding, Xueqin; Lin, Xiao; Huang, Songyun; Liu, Xiaojie

    2013-11-15

    Eight kinds of green ionic liquids were synthesized, and an ionic liquid aqueous two-phase system (ILATPS) based on 1,1,3,3-tetramethylguandine acrylate (TMGA) guanidine ionic liquid was first time studied for the extraction of proteins. Single factor experiments proved that the extraction efficiency of bovine serum albumin (BSA) was influenced by the mass of IL, K2HPO4 and BSA, also related to the separation time and temperature. The optimum conditions were determined through orthogonal experiment by the five factors described above. The results showed that under the optimum conditions, the extraction efficiency could reach up to 99.6243%. The relative standard deviations (RSD) of extraction efficiencies in precision experiment, repeatability experiment and stability experiment were 0.8156% (n=5), 1.6173% (n=5) and 1.6292% (n=5), respectively. UV-vis and FT-IR spectra confirmed that there were no chemical interactions between BSA and ionic liquid in the extraction process, and the conformation of the protein was not changed after extraction. The conductivity, DLS and TEM were combined to investigate the microstructure of the top phase and the possible mechanism for the extraction. The results showed that hydrophobic interaction, hydrogen bonding interaction and the salt out effect played important roles in the transferring process, and the aggregation and embrace phenomenon was the main driving force for the separation. All these results proved that guanidine ionic liquid-based ATPSs have the potential to offer new possibility in the extraction of proteins.

  15. Partitioning in aqueous two-phase systems: Analysis of strengths, weaknesses, opportunities and threats.

    Science.gov (United States)

    Soares, Ruben R G; Azevedo, Ana M; Van Alstine, James M; Aires-Barros, M Raquel

    2015-08-01

    For half a century aqueous two-phase systems (ATPSs) have been applied for the extraction and purification of biomolecules. In spite of their simplicity, selectivity, and relatively low cost they have not been significantly employed for industrial scale bioprocessing. Recently their ability to be readily scaled and interface easily in single-use, flexible biomanufacturing has led to industrial re-evaluation of ATPSs. The purpose of this review is to perform a SWOT analysis that includes a discussion of: (i) strengths of ATPS partitioning as an effective and simple platform for biomolecule purification; (ii) weaknesses of ATPS partitioning in regard to intrinsic problems and possible solutions; (iii) opportunities related to biotechnological challenges that ATPS partitioning may solve; and (iv) threats related to alternative techniques that may compete with ATPS in performance, economic benefits, scale up and reliability. This approach provides insight into the current status of ATPS as a bioprocessing technique and it can be concluded that most of the perceived weakness towards industrial implementation have now been largely overcome, thus paving the way for opportunities in fermentation feed clarification, integration in multi-stage operations and in single-step purification processes.

  16. Measurement and Correlation of Equilibrium Data for Aqueous Two-phase System Ethanol+Water+K2HPO4

    Institute of Scientific and Technical Information of China (English)

    LIN Jin-qing; TAN Ping-hua; JIN Chun-ying; LI Ming-chun

    2004-01-01

    The isothermal solubility data of aqueous two-phase system ethanol+water+K2HPO4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very well. An experimental procedure for measuring the liquid-liquid equilibrium data of the aqueous two-phase system was proposed, in which the concentrations of the coexisting phases were determined with the corresponding densities of the solution. The tie lines were satisfactorily described by using the Othmer Tobias and Bancroft equations.

  17. Recovery of crocins from saffron stigmas (Crocus sativus) in aqueous two-phase systems.

    Science.gov (United States)

    Montalvo-Hernández, Bertha; Rito-Palomares, Marco; Benavides, Jorge

    2012-05-04

    Crocins are carotenoid derivates that have recently attracted the interest of the scientific community due to their nutraceutical properties. Saffron (dry Crocus sativus stigmas) is one of the main known sources of crocins. In this study the potential use of aqueous two-phase system (ATPS) for the extraction of crocins from C. sativus stigmas was evaluated. The partitioning behavior of crocins in different types of ATPS (polymer-polymer, polymer-salt, alcohol-salt and ionic liquid-salt) was evaluated. Ethanol-potassium phosphate ATPS were selected based on their high top phase recovery yield and low cost of system constituents. The evaluation and optimization of system parameters rendered conditions (V(R)=3.2, ethanol 19.8% (w/w), potassium phosphate 16.5% (w/w), TLL of 25% (w/w), 0.1M NaCl and 2% (w/w) of sample load) under which more than 75% of total crocins were recovered in the top (ethanol rich) phase, whereas the wasted stigmas accumulated in the bottom phase. Lastly, a comparison between an optimized solid-liquid extraction using ethanol:water as solvent and ATPS was conducted demonstrating that similar yields are achieved with both strategies (76.89 ± 18% and 79.27 ± 1.6%, respectively). However, ATPS rendered a higher extraction selectivity of 1.3 ± 0.04 mg of crocins for each mg of phenolic compound, whereas ethanolic extraction showed a selectivity of 0.87 ± 0.01. The results reported herein demonstrate the potential application of ATPS, particularly ethanol-potassium phosphate systems, for the recovery of crocins from C. sativus stigmas.

  18. Ionic interaction of oral streptococcal bacteria studied by partition in an aqueous polymer two-phase system.

    Science.gov (United States)

    Westergren, G

    1981-01-01

    The net surface charge of various oral streptococci were assessed by aqueous two-phase partitioning in a dextran-polyethylene glycol system. Great variability was found among individual strains within all species tested. Type 1 strains of Streptococcus sanguis serotypes which have been found to be more adherent, exposed a lower negative net surface charge than Type 2 strains.

  19. Cloud Point and Liquid-Liquid Equilibrium Behavior of Thermosensitive Polymer L61 and Salt Aqueous Two-Phase System.

    Science.gov (United States)

    Rao, Wenwei; Wang, Yun; Han, Juan; Wang, Lei; Chen, Tong; Liu, Yan; Ni, Liang

    2015-06-25

    The cloud point of thermosensitive triblock polymer L61, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO), was determined in the presence of various electrolytes (K2HPO4, (NH4)3C6H5O7, and K3C6H5O7). The cloud point of L61 was lowered by the addition of electrolytes, and the cloud point of L61 decreased linearly with increasing electrolyte concentration. The efficacy of electrolytes on reducing cloud point followed the order: K3C6H5O7 > (NH4)3C6H5O7 > K2HPO4. With the increase in salt concentration, aqueous two-phase systems exhibited a phase inversion. In addition, increasing the temperature reduced the concentration of salt needed that could promote phase inversion. The phase diagrams and liquid-liquid equilibrium data of the L61-K2HPO4/(NH4)3C6H5O7/K3C6H5O7 aqueous two-phase systems (before the phase inversion but also after phase inversion) were determined at T = (25, 30, and 35) °C. Phase diagrams of aqueous two-phase systems were fitted to a four-parameter empirical nonlinear expression. Moreover, the slopes of the tie-lines and the area of two-phase region in the diagram have a tendency to rise with increasing temperature. The capacity of different salts to induce aqueous two-phase system formation was the same order as the ability of salts to reduce the cloud point.

  20. Study on PEG-(NH4)2SO4 Aqueous Two-Phase System and Distribution Behavior of Drugs

    Institute of Scientific and Technical Information of China (English)

    LI, Lei(李蕾); HE, Chi-Yang(何池洋); LI, She-Hong(李社红); LIU, Feng(刘锋); SU, Shun(苏顺); KONG, Xiang-Xu(孔祥旭); LI, Na(李娜); LI, Ke-An(李克安)

    2004-01-01

    The distribution behavior of chlorpromazine hydrochloride (CPZ), procaine hydrochloride (PCN) and procaine amide hydrochloride (PCNA) in polyethylene glycol (PEG800 or PEG1500)-(NH4)2SO4 aqueous two-phase systems has been investigated. The result shows that the PEG-(NH4)2SO4 aqueous two-phase system has potential extraction capability in small molecular drug separation. In PEG800-(NH4)2SO4 system, the extraction efficiencies (E)of CPZ, PCN and PCNA amount to 92.8%, 74.5% and 74.4%, respectively, with the distribution coefficients (KD)being 25.7, 5.9 and 5.8, correspondingly. In PEG1500-(NH4)2SO4 system, the extraction efficiencies (E) of CPZ,PCN and PCNA are 93.7%, 71.3% and 63.2%, respectively, with distribution coefficients (KD) of 39.6, 6.6 and 5.0,correspondingly. Based on the study on ultraviolet and fluorescence spectra and also distribution behavior of the drugs in PEG-(NH4)2SO4 aqueous two-phase system, extraction mechanism was further proposed that both hydrogen bond and hydrophobic interaction are involved in extraction.

  1. Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system.

    Science.gov (United States)

    Sun, Li-Hui; Jiang, Bo; Xiu, Zhi-Long

    2009-03-01

    A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.

  2. Application of surface response analysis to the optimization of penicillin acylase purification in aqueous two-phase systems

    OpenAIRE

    2002-01-01

    Penicillin acylase purification from an Escherichia coli crude extract using PEG 3350 – sodium citrate aqueous two phase systems was optimized. An experimental design was used to evaluate the influence of PEG, sodium citrate and sodium chloride on the purification parameters. A central composite design was defined centred on the previously found conditions for highest purification from an osmotic shock extract. Mathematical models for the partition coefficient of protein and enzyme, balance o...

  3. Partition of Chiral pharmaceutical intermediate R(-)-Mandelic Acid in Aqueous Two-Phase System of Poly(ethylene glycol)-Ammonium Sulfate

    Institute of Scientific and Technical Information of China (English)

    Xu Xiaoping; Li Zhongqin; Chen Jiebo; Huang Xinghua

    2004-01-01

    An aqueous two-phase system of poly (ethylene glycol)-ammonium sulfate was employed to separate R (-)-mandelic acid.The result showed that R (-)-mandelic acid has priority to partition in PEG-rich top phase. This indicated that aqueous two-phase is a very suitable system for separation of R(-)-mandelic acid.

  4. Multi-stage mixer-settler planet centrifuge. Preliminary studies on partition of macromolecules with organic-aqueous and aqueous-aqueous two-phase solvent systems.

    Science.gov (United States)

    Ito, Y; Zhang, T Y

    1988-03-11

    A rotary-seal-free planetary centrifuge holds a separation column which consists of multiple partition units (ca. 200) connected in series with transfer tubes. In the cavity of each partition unit the transfer tube extends to form a mixer which vibrates to stir the contents under an oscillating force field generated by the planetary motion of the centrifuge. Consequently, solutes locally introduced at the inlet of the column are subjected to an efficient partition process in each partition unit and separated according to their partition coefficients. The mixer tube equipped with a flexible silicone rubber joint was found to produce excellent results for partition with viscous polymer phase systems. The capability of the method was demonstrated on separation of cytochrome c and lysozyme using a PEG-aqueous dibasic potassium phosphate-aqueous two-phase solvent system.

  5. Use of multifactorial analysis to develop aqueous two-phase systems for isolation of non-native IGF-I.

    Science.gov (United States)

    Hart, R A; Ogez, J R; Builder, S E

    1995-04-01

    A high yield procedure was developed to solubilize and extract IGF-I from recombinant E. coli by adding chaotrope and disulfide reductant to alkaline fermentation broth. To enhance centrifugation performance and recovery yield, a salt/polymer aqueous two-phase extraction procedure was developed whereby soluble non-native IGF-I and biomass solids are enriched in separate liquid phases. To develop this extraction system a multifactorial experimental approach was used to simultaneously map the phase diagram and identify conditions to suitably partition IGF-I and cell remnants. The presence of urea in these systems tended to disrupt two-phase formation and solids sedimentation. This, in turn, constrained the concentrations of phase forming solutes which could be effectively used. Systems containing low levels of salt (less than about 4% w/w) and polymer (less than about 10% w/w) did not form two phases. Systems containing high levels of salt (greater than about 7% w/w) and polymer (greater than about 18% w/w) formed two phases with floating solids. Intermediate levels of salt (between about 4% and 7% w/w) and polymer (between about 10% and 18% w/w) formed two phases in which solids were enriched in the heavy phase. Systems in this latter desired category were produced with a variety of different salts and polymers and all enriched non-native IGF-I in the light phase. Highest recovery yield (about 90%) was obtained with systems composed of 5% sodium sulfate and 14% PEG-8000.(ABSTRACT TRUNCATED AT 250 WORDS)

  6. A resolution approach of racemic phenylalanine with aqueous two-phase systems of chiral tropine ionic liquids.

    Science.gov (United States)

    Wu, Haoran; Yao, Shun; Qian, Guofei; Yao, Tian; Song, Hang

    2015-10-30

    Aqueous two-phase systems (ATPS) based on tropine type chiral ionic liquids and inorganic salt solution were designed and prepared for the enantiomeric separation of racemic phenylalanine. The phase behavior of IL-based ATPS was comprehensive investigated, and phase equilibrium data were correlated by Merchuk equation. Various factors were also systematically investigated for their influence on separation efficiency. Under the appropriate conditions (0.13g/g [C8Tropine]pro, 35mg/g Cu(Ac)2, 20mg/g d,l-phenylalanine, 0.51g/g H2O and 0.30g/g K2HPO4), the enantiomeric excess value of phenylalanine in solid phase (mainly containing l-enantiomer) was 65%. Finally, the interaction mechanism was studied via 1D and 2D NMR. The results indicate that d-enantiomer of phenylalanine interacts more strongly with chiral ILs and Cu(2+) based on the chiral ion-pairs space coordination mechanism, which makes it tend to remain in the top IL-rich phase. By contrast, l-enantiomer is transferred into the solid phase. Above chiral ionic liquids aqueous two-phase systems have demonstrated obvious resolution to racemic phenylalanine and could be promising alterative resolution approach for racemic amino acids in aqueous circumstance.

  7. Studies of efficiency in a perforated rotating disc contactor using a polymer-polymer aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    L. A. Sarubbo

    2005-09-01

    Full Text Available The mass transfer process in a perforated rotating disc contactor (PRDC using a polymer-polymer aqueous two-phase system was investigated. The results show that the efficiency did not show a regular trend with the increase of the dispersed phase velocity and increased with the rotation velocity. The separation efficiency was higher for three rotating discs than for four discs. The increase in tie-line length decreased the efficiency. The separation efficiency reached high values, about 96% under conditions studied in this work.

  8. Thermodynamic modelling of phase equilibrium for water + poly(Ethylene glycol + salt aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    R.A.G. Sé

    2002-04-01

    Full Text Available The NRTL (nonrandom, two-liquid model, expressed in mass fraction instead of mole fraction, was used to correlate liquid-liquid equilibria for aqueous two-phase polymer-salt solutions. New interaction energy parameters for this model were determined using reported data on the water + poly(ethylene glycol + salt systems, with different molecular masses for PEG and the salts potassium phosphate, sodium sulfate, sodium carbonate and magnesium sulfate. The correlation of liquid-liquid equilibrium is quite satisfactory.

  9. Partition of volatile compounds in pea globulin-maltodextrin aqueous two-phase system.

    Science.gov (United States)

    Nguyen, Thanh Dat; Lafarge, Céline; Murat, Chloé; Mession, Jean-Luc; Cayot, Nathalie; Saurel, Rémi

    2014-12-01

    This study is based on the assumption that the off-flavour of pea proteins might be decreased using the retention of volatile compounds by a mixture with another biopolymer. The partition of volatile compounds in an aqueous system containing pea protein and maltodextrins was followed under thermodynamic incompatibility conditions. Firstly, the phase diagram of the system was established. Then, the partition of aroma compounds between the phase rich in protein and the phase rich in maltodextrin was measured by SPME-GC-MS. There was a transfer of volatile compounds during phase separation. Variations of pH were also used to vary the retention of volatile compounds by proteins. The concentration of volatile compounds in protein solution at pH 2.4 was higher than at pH 7.2. It was possible to increase the transfer of volatile compounds from the phase rich in protein to the phase rich in maltodextrin using the effect of pH on protein denaturation.

  10. Effect of sodium chloride on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems.

    Science.gov (United States)

    da Silva, Nuno R; Ferreira, Luisa A; Madeira, Pedro P; Teixeira, José A; Uversky, Vladimir N; Zaslavsky, Boris Y

    2015-12-18

    Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215M NaCl and 0.5M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215M NaCl system, all in 0.01M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive.

  11. Purification of papain by metal affinity partitioning in aqueous two-phase polyethylene glycol/sodium sulfate systems.

    Science.gov (United States)

    Jiang, Zhi-Guo; Zhang, Hai-De; Wang, Wei-Tao

    2015-05-01

    A simple and inexpensive aqueous two-phase affinity partitioning system using metal ligands was introduced to improve the selectivity of commercial papain extraction. Polyethylene glycol 4000 was first activated using epichlorohydrin, then it was covalently linked to iminodiacetic acid. Finally, the specific metal ligand Cu(2+) was attached to the polyethylene glycol-iminodiacetic acid. The chelated Cu(2+) content was measured by atomic absorption spectrometry as 0.88 mol/mol (polyethylene glycol). The effects on the purification at different conditions, including polyethylene glycol molecular weight (2000, 4000, and 6000), concentration of phase-forming components (polyethylene glycol 12-20% w/w and sodium sulfate 12-20%, w/w), metal ligand type, and concentration, system pH and the commercial papain loading on papain extraction, were systematically studied. Under optimum conditions of the system, i.e. 18% w/w sodium sulfate, 18% w/w polyethylene glycol 4000, 1% w/w polyethylene glycol-iminodiacetic acid-Cu(2+) and pH 7, a maximum yield of 90.3% and a degree of purification of 3.6-fold were obtained. Compared to aqueous two phase extraction without ligands, affinity partitioning was found to be an effective technique for the purification of commercial papain with higher extraction efficiency and degree of purification.

  12. Isolation and fractionation of CHO chromosomes in aqueous two phase systems using charged polymers and base specific macroligands.

    Science.gov (United States)

    Klaar, J; Kula, M R

    1986-02-01

    Chromosomes were isolated in a preparative scale by synchronisation of CHO cells with a double Thymidine block followed by an arrest in the metaphase by addition of Colcemid. Under proper cultivation conditions a mitotic index of 77% total cells could be routinely achieved. Bulk chromosome preparations free of nuclei and other subcellular particles have been obtained by low speed centrifugation followed by a 60 transfer countercurrent distribution using aqueous two phase systems composed of polyethylenglycol and dextran. The partition of CHO chromosomes previously purified in aqueous two phase systems were studied further to develop a protocol for the separation and isolation of individual chromosomes. Partition experiments with chromosomes changing the electrostatic phase potential by addition of charged PEG-derivatives suggest the existence of relatively highly charged chromosome groups. Most promising results with regard to separation were obtained using two PEG-derivatives, which interact specifically with the bases in DNA. For this affinity partitioning a GC- and AT-specific macroligand were employed. Comparing CCD's using each of these ligands information on the GC and AT content of exposed DNA in the chromosomes groups could be derived, demonstrating that specific sequences of DNA are accessible at the surface of metaphase chromosomes.

  13. Influence of system and process parameters on partitioning of cheese whey proteins in aqueous two-phase systems.

    Science.gov (United States)

    Rito-Palomares, M; Hernandez, M

    1998-06-26

    A practical study is described to characterise some problems encountered in the application of aqueous two-phase systems (ATPS) to protein recovery. These factors include practical design of extraction stages and the impact of ATPS compounding methods and biological suspension upon process performance. They were addressed using the recovery of whey proteins as a model. The known effects of system parameters (i.e. tie-line length, volume ratio and system pH) were exploited to define the specific operating conditions of a two-stage ATPS process for the recovery of whey proteins. The partition of whey proteins in ATPS assembled using different methods resulted in changes in the partition coefficient of the proteins. Such changes were associated with the initial location of the proteins in the polymer or salt-rich solutions of the ATPS. Cheese whey loaded into the ATPS caused the displacement of the binodal curve from the origin. Such behaviour was attributed to the residual fat present in the whey. These findings highlight those factors perceived as negative constraints on the wider adoption of ATPS processes for protein recovery from complex biological systems.

  14. Study on aqueous two-phase systems of the mixture SDS/CTAB surfactants

    Institute of Scientific and Technical Information of China (English)

    LI Ying; CHEN Yah-ming; ZHAO Kong-shuang; Takumi HIKIDA

    2004-01-01

    The phenomenon of two dilute aqueous phases composed of sodium dodecylsulfate (SDS) and cetyltrimethyl ammonium bromide(CTAB) was investigated under various conditions such as concentrations and molarratios of the two surfactants, the addition of sodium chloride and temperature. Vesicles formation was found in theboth phases by TEM image.

  15. Design of aqueous two-phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis.

    Science.gov (United States)

    Rajendran, Vivek; Puvendran, Kirubhakaran; Guru, Bharath Raja; Jayaraman, Guhan

    2016-02-01

    Hyaluronic acid has a wide range of biomedical applications and its commercial value is highly dependent on its purity and molecular weight. This study highlights the utility of aqueous two-phase separation as a primary recovery step for hyaluronic acid and for removal of major protein impurities from fermentation broths. Metabolically engineered cultures of a lactate dehydrogenase mutant strain of Lactococcus lactis (L. lactis NZ9020) were used to produce high-molecular-weight hyaluronic acid. The cell-free fermentation broth was partially purified using a polyethylene glycol/potassium phosphate system, resulting in nearly 100% recovery of hyaluronic acid in the salt-rich bottom phase in all the aqueous two-phase separation experiments. These experiments were optimized for maximum removal of protein impurities in the polyethylene glycol rich top phase. The removal of protein impurities resulted in substantial reduction of membrane fouling in the subsequent diafiltration process, carried out with a 300 kDa polyether sulfone membrane. This step resulted in considerable purification of hyaluronic acid, without any loss in recovery and molecular weight. Diafiltration was followed by an adsorption step to remove minor impurities and achieve nearly 100% purity. The final hyaluronic acid product was characterized by Fourier-transform IR and NMR spectroscopy, confirming its purity.

  16. Can affinity interactions influence the partitioning of glucose-6-phosphate dehydrogenase in two-phase aqueous micellar systems?

    Directory of Open Access Journals (Sweden)

    André M. Lopes

    2008-01-01

    Full Text Available In this work, we provide an investigation of the role and strength of affinity interactions on the partitioning of the glucose-6-phosphate dehydrogenase in aqueous two-phase micellar systems. These systems are constituted of micellar surfactant solutions and offer both hydrophobic and hydrophilic environments, providing selectivity to biomolecules. We studied G6PD partitioning in systems composed of the nonionic surfactants, separately, in the presence and absence of affinity ligands. We observed that G6PD partitions to the micelle-poor phase, owing to the strength of excluded-volume interactions in these systems that drive the protein to the micelle-poor phase, where there is more free volume available.

  17. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Xueqin; Wang, Yuzhi, E-mail: wyzss@hnu.edu.cn; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-01

    Graphical abstract: - Highlights: • A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been synthesized. • Functional guanidinium ionic liquid aqueous two-phase systems have been first designed for the purification of protein. • Mechanisms and performances of the process were researched. • Simple, green, safety and presents better purified ability than ordinary process. • A potential efficient platform for protein purification and related studies. - Abstract: A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by {sup 1}H nuclear magnetic resonance ({sup 1}H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV–vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV–vis spectrophotometer, Fourier-transform infrared

  18. Measurement and Correlation of Partition Coefficients of Baicalin in EOPO/Salt Aqueous Two-Phase Systems

    Institute of Scientific and Technical Information of China (English)

    李伟; 朱自强

    2002-01-01

    The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide(EOPO)/salt aqueous two-phase systems at 298.15K,It was found that most of baicalin partitioned into EOPO-rich phase.The partition coefficients of baicalin varied from 10 to 120.The effect of various factors,including tie-line lngth,salt composition,molecular weight of EOPO,and solution pH,on the partition behavior was investigated on EOPO/salt systems.Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsu model.Good agreement with experimental data is obtained.The average relative deviations are less than 5.0%.

  19. Aqueous two-phase systems: A simple methodology to obtain mixtures enriched in main toxins of Bothrops alternatus venom.

    Science.gov (United States)

    Gomez, Gabriela; Leiva, Laura; Nerli, Bibiana Beatriz

    2016-08-01

    Phospholipase A2 (PLA2) and protease (P) are enzymes responsible of myotoxic, edematogenic and hemostasis disorder effects observed in the envenomation by Bothrops alternatus pitviper. Their partitioning coefficient (Kp) in different polyethyleneglycol/potassium phosphate aqueous two-phase systems (ATPSs) was determined in order to both achieve a better understanding of the partitioning mechanism and define optimal conditions for toxin isolation. Polyethyleneglycols (PEGs) of molecular weights 1000; 3350; 6000 and 8000; different temperatures (5, 20 and 37 °C) and phase volume ratios of 0.5; 1 and 2 were assayed. PLA2 partitioned preferentially to the top phase while P mainly distributed to the bottom phase. Either entropically- or enthalpically-driven mechanisms were involved in each case (PLA2 and P). The aqueous two-phase system formed by PEG of MW 3350 (12.20% wt/wt) and KPi pH 7.0 (11.82% wt/wt) with a volume ratio of one and a load of 1.25 mg of venom/g of system showed to be the most efficient to recover both enzymes. It allowed obtaining the 72% of PLA2 in the top phase with a purification factor of 2 and the 82% of P at the bottom phase simultaneously. A further adsorption batch step with DEAE-cellulose was used to remove satisfactorily the PEG from the top phase and recover the active PLA2. The proposed methodology is simple, inexpensive, and only requires professionals trained in handling basic laboratory equipment. It could be easily adoptable by developing countries in which the snakebite accidents cause considerable morbidity and mortality.

  20. Phase Diagrams of the Aqueous Two-Phase Systems of Poly(ethylene glycol/Sodium Polyacrylate/Salts

    Directory of Open Access Journals (Sweden)

    Adalberto Pessoa Junior

    2011-03-01

    Full Text Available Aqueous two-phase systems consisting of polyethylene glycol (PEG, sodium polyacrylate (NaPAA, and a salt have been studied. The effects of the polymer size, salt type (NaCl, Na2SO4, sodium adipate and sodium azelate and salt concentrations on the position of the binodal curve were investigated. The investigated PEG molecules had a molar mass of 2,000 to 8,000 g/mol, while that of NaPAA was 8,000 g/mol. Experimental phase diagrams, and tie lines and calculated phase diagrams, based on Flory-Huggins theory of polymer solutions are presented. Due to strong enthalpic and entropic balancing forces, the hydrophobicity of the added salt has a strong influence on the position of the binodal, which could be reproduced by model calculations.

  1. Design of functional guanidinium ionic liquid aqueous two-phase systems for the efficient purification of protein.

    Science.gov (United States)

    Ding, Xueqin; Wang, Yuzhi; Zeng, Qun; Chen, Jing; Huang, Yanhua; Xu, Kaijia

    2014-03-07

    A series of novel cationic functional hexaalkylguanidinium ionic liquids and anionic functional tetraalkylguanidinium ionic liquids have been devised and synthesized based on 1,1,3,3-tetramethylguanidine. The structures of the ionic liquids (ILs) were confirmed by (1)H nuclear magnetic resonance ((1)H NMR) and 13C nuclear magnetic resonance (13C NMR) and the production yields were all above 90%. Functional guanidinium ionic liquid aqueous two-phase systems (FGIL-ATPSs) have been first designed with these functional guanidinium ILs and phosphate solution for the purification of protein. After phase separation, proteins had transferred into the IL-rich phase and the concentrations of proteins were determined by measuring the absorbance at 278 nm using an ultra violet visible (UV-vis) spectrophotometer. The advantages of FGIL-ATPSs were compared with ordinary ionic liquid aqueous two-phase systems (IL-ATPSs). The proposed FGIL-ATPS has been applied to purify lysozyme, trypsin, ovalbumin and bovine serum albumin. Single factor experiments were used to research the effects of the process, such as the amount of ionic liquid (IL), the concentration of salt solution, temperature and the amount of protein. The purification efficiency reaches to 97.05%. The secondary structure of protein during the experimental process was observed upon investigation using UV-vis spectrophotometer, Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism spectrum (CD spectrum). The precision, stability and repeatability of the process were investigated. The mechanisms of purification were researched by dynamic light scattering (DLS), determination of the conductivity and transmission electron microscopy (TEM). It was suggested that aggregation and embrace phenomenon play a significant role in the purification of proteins. All the results show that FGIL-ATPSs have huge potential to offer new possibility in the purification of proteins.

  2. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA

    Science.gov (United States)

    Frampton, John P.; White, Joshua B.; Simon, Arlyne B.; Tsuei, Michael; Paczesny, Sophie; Takayama, Shuichi

    2014-05-01

    Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because the detection antibody solutions are co-localized only to corresponding surface-immobilized capture antibody spots. This multiplexing technique is validated using plasma samples from allogeneic bone marrow recipients. Patients with acute graft versus host disease (GVHD), a common and serious condition associated with allogeneic bone marrow transplantation, display higher mean concentrations for four multiplexed biomarkers (HGF, elafin, ST2 and TNFR1) relative to healthy donors and transplant patients without GVHD. The antibody co-localization capability of this technology is particularly useful when using inherently cross-reactive reagents such as polyclonal antibodies, although monoclonal antibody cross-reactivity can also be reduced. Because ATPS-ELISA adapts readily available antibody reagents, plate materials and detection instruments, it should be easily transferable into other research and clinical settings.

  3. Formation and characteristics of aqueous two-phase systems formed by a cationic surfactant and a series of ionic liquids.

    Science.gov (United States)

    Wei, Xi-Lian; Wang, Xiu-Hong; Ping, A-Li; Du, Pan-Pan; Sun, De-Zhi; Zhang, Qing-Fu; Liu, Jie

    2013-11-15

    Aqueous two-phase systems (ATPS) were obtained in the aqueous mixtures of a cationic surfactant and a series of ionic liquids (ILs). The effects of IL structure, temperature and additives on the phase separation were systematically investigated. The microstructures of some ATPS were observed by freeze-fracture replication technique. Lyotropic liquid crystal was found in the bottom phase besides micelles under different conditions. Remarkably, both IL structure and additives profoundly affected the formation and properties of the ATPSs. The phase separation can be attributed to the existence of different aggregates and the cation-π interactions of the cationic surfactant with the ILs, which has a significant role in the formation of ATPS. The extraction capacity of the studied ATPS was also evaluated through their application in the extraction of two biosubstances. The results indicate that the ILs with BF4(-) as anion show much better extraction efficiencies than the corresponding ILs with Br(-) as anion do under the same conditions. l-Tryptophan was mainly distributed into the NPTAB-rich phase, while methylene blue and capsochrome were mainly in the IL-rich phase.

  4. Analysis of partitioning of organic compounds and proteins in aqueous polyethylene glycol-sodium sulfate aqueous two-phase systems in terms of solute-solvent interactions.

    Science.gov (United States)

    da Silva, Nuno R; Ferreira, Luisa A; Madeira, Pedro P; Teixeira, José A; Uversky, Vladimir N; Zaslavsky, Boris Y

    2015-10-09

    Partition behavior of nine small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.5M osmolyte (sorbitol, sucrose, trehalose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate system, all in 0.01M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. It was found out that the partition coefficient of all compounds examined (including proteins) may be described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system differ from those in polyethylene glycol-dextran system.

  5. Phase behavior and molecular dynamics simulation studies of new aqueous two-phase separation systems induced by HEPES buffer.

    Science.gov (United States)

    Taha, Mohamed; Khoiroh, Ianatul; Lee, Ming-Jer

    2013-01-17

    Here, for the first time, we show that with addition of a biological buffer, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), into aqueous solutions of tetrahydrofuran (THF), 1,3-dioxolane, 1,4-dioxane, 1-propanol, 2-propanol, tert-butanol, acetonitrile, or acetone, the organic solvent can be excluded from water to form a new liquid phase. The phase diagrams have been determined at ambient temperature. In order to understand why and how a zwitterion solute (HEPES) induced phase separation of the investigated systems, molecular dynamics (MD) simulation studies are performed for HEPES + water + THF system. The MD simulations were conducted for the aqueous mixtures with 12 different compositions. The reliability of the simulation results of HEPES in pure water and beyond the phase separation mixtures was justified by comparing the densities obtained from MD with the experimental values. The simulation results of HEPES in pure THF and in a composition inside the phase separation region were justified qualitatively. Interestingly, all HEPES molecules entirely aggregated in pure THF. This reveals that HEPES is insoluble in pure THF, which is consistent with the experimental results. Even more interestingly, the MD simulation for the mixture with composition inside the phase separation region showed the formation of two phases. The THF molecules are squeezed out from the water network into a new liquid phase. The hydrogen bonds (HBs), HB lifetime, HB Gibbs energy (ΔG), radial distribution functions (RDFs), coordination numbers (CNs), electrostatic interactions, and the van der Waals interactions between the different species have been analyzed. Further, MD simulations for the other phase separation systems by choosing a composition inside the two liquids region for each system were also simulated. Our findings will therefore pave the way for designing new benign separation auxiliary agents.

  6. Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system.

    Science.gov (United States)

    Tan, Zhi-jian; Li, Fen-fang; Xu, Xue-lei

    2013-08-01

    An alcohol/salt aqueous two-phase system (ATPS) composed of 1-propanol and (NH4)2SO4 was employed to purify anthraquinones (AQs) extracted from Aloe vera L. The main influencing system parameters such as type of alcohol, type and concentration of salt, temperature and pH were investigated in detail. Under the optimal extraction conditions, AQs can be extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in ATPS. Temperature also played a great role in the partitioning. After ATPS extraction, alcohol can be recycled by evaporation; moreover, salt can be recycled by dilution crystallization method. Compared with other liquid-liquid extractions, this alcohol/salt system is much simpler, lower in cost with easier recovery of phase-forming components, which has the potential scale-up in down-processing of active ingredients in plant.

  7. Isolation of natural red colorants from fermented broth using ionic liquid-based aqueous two-phase systems.

    Science.gov (United States)

    Ventura, Sónia P M; Santos-Ebinuma, Valéria C; Pereira, Jorge F B; Teixeira, Maria F S; Pessoa, Adalberto; Coutinho, João A P

    2013-05-01

    There is a growing demand for natural colorants. This is prompting the search for new alternative and "benign" separation systems allowing higher recoveries, extraction yields, and selectivities. This work investigates the use of aqueous two-phase systems (ATPS) based on ionic liquids as extraction processes for the recovery of red colorants from the fermented broth of Penicillium purpurogenum DPUA 1275. Several ATPS based on quaternary ammonium and imidazolium were studied in this work aiming at separating the red colorants produced from the remaining colorants and contaminant proteins present in the fermented broth. The results suggest that the red colorants can be isolated by an appropriate manipulation of some of the process conditions, such as the use of quaternary ammonium with short alkyl chains, alkaline media, and short tie-line lengths (extraction point systems with lower concentrations of ionic liquid). These conditions allow large partition coefficients for the red colorants (K red = 24.4 ± 2.3), high protein removal (60.7 ± 2.8 %) and selectivity parameters (S red/prot = 10.05).

  8. Characterization of aqueous two phase systems by combining lab-on-a-chip technology with robotic liquid handling stations.

    Science.gov (United States)

    Amrhein, Sven; Schwab, Marie-Luise; Hoffmann, Marc; Hubbuch, Jürgen

    2014-11-07

    Over the last decade, the use of design of experiment approaches in combination with fully automated high throughput (HTP) compatible screenings supported by robotic liquid handling stations (LHS), adequate fast analytics and data processing has been developed in the biopharmaceutical industry into a strategy of high throughput process development (HTPD) resulting in lower experimental effort, sample reduction and an overall higher degree of process optimization. Apart from HTP technologies, lab-on-a-chip technology has experienced an enormous growth in the last years and allows further reduction of sample consumption. A combination of LHS and lab-on-a-chip technology is highly desirable and realized in the present work to characterize aqueous two phase systems with respect to tie lines. In particular, a new high throughput compatible approach for the characterization of aqueous two phase systems regarding tie lines by exploiting differences in phase densities is presented. Densities were measured by a standalone micro fluidic liquid density sensor, which was integrated into a liquid handling station by means of a developed generic Tip2World interface. This combination of liquid handling stations and lab-on-a-chip technology enables fast, fully automated, and highly accurate density measurements. The presented approach was used to determine the phase diagram of ATPSs composed of potassium phosphate (pH 7) and polyethylene glycol (PEG) with a molecular weight of 300, 400, 600 and 1000 Da respectively in the presence and in the absence of 3% (w/w) sodium chloride. Considering the whole ATPS characterization process, two complete ATPSs could be characterized within 24h, including four runs per ATPS for binodal curve determination (less than 45 min/run), and tie line determination (less than 45 min/run for ATPS preparation and 8h for density determination), which can be performed fully automated over night without requiring man power. The presented methodology provides

  9. One-Step Generation of Cell-Encapsulating Compartments via Polyelectrolyte Complexation in an Aqueous Two Phase System.

    Science.gov (United States)

    Hann, Sarah D; Niepa, Tagbo H R; Stebe, Kathleen J; Lee, Daeyeon

    2016-09-28

    Diverse fields including drug and gene delivery and live cell encapsulation require biologically compatible encapsulation systems. One widely adopted means of forming capsules exploits cargo-filled microdroplets in an external, immiscible liquid phase that are encapsulated by a membrane that forms by trapping of molecules or particles at the drop surface, facilitated by the interfacial tension. To eliminate the potentially deleterious oil phase often present in such processes, we exploit the aqueous two phase system of poly(ethylene glycol) (PEG) and dextran. We form capsules by placing dextran-rich microdroplets in an external PEG-rich phase. Strong polyelectrolytes present in either phase form complexes at the drop interface, thereby forming a membrane encapsulating the fluid interior. This process requires considerable finesse as both polyelectrolytes are soluble in either the drop or external phase, and the extremely low interfacial tension is too weak to provide a strong adsorption site for these molecules. The key to obtaining microcapsules is to tune the relative fluxes of the two polyelectrolytes so that they meet and complex at the interface. We identify conditions for which complexation can occur inside or outside of the drop phase, resulting in microparticles or poor encapsulation, respectively, or when properly balanced, at the interface, resulting in microcapsules. The resulting microcapsules respond to the stimuli of added salts or changes in osmotic pressure, allowing perturbation of capsule permeability or triggered release of capsule contents. We demonstrate that living cells can be sequestered and interrogated by encapsulating Pseudomonas aeruginosa PAO1 and using a Live/Dead assay to assess their viability. This method paves the way to the formation of a broad variety of versatile functional membranes around all aqueous capsules; by tuning the fluxes of complexing species to interact at the interface, membranes comprising other complexing

  10. 双水相体系在无机盐分离中的应用%Application of Aqueous - two Phase System in the Separation of Inorganic Salts

    Institute of Scientific and Technical Information of China (English)

    史许娜; 韩清华

    2016-01-01

    Different separation methods of potassium chloride and ammonium chloride mixture are ana-lyzed. The present situation for separating mixed solution of potassium chloride and ammonium chloride by aqueous two - phase system(1 - propanol - KCl - NH4 Cl - H2 O aqueous two - phase system)is intro-duced,and the application of aqueous two - phase system is expanded.%分析了氯化钾和氯化铵混合溶液的不同分离方法,介绍了双水相体系(正丙醇—氯化钾—氯化铵—水双水相体系)对该混合溶液的分离现状,拓展了双水相体系的应用范围。

  11. Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design

    Directory of Open Access Journals (Sweden)

    Francine Silva Antelo

    2015-02-01

    Full Text Available C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS of polyethylene glycol (PEG/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w PEG 1500 or 4% (w/w PEG 8000 (g/gmol and 23 or 22.5% (w/w of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the purification factor for the system with PEG 1500. The ATPS with 4% (w/w PEG 4000 or 4% (w/w PEG 6000 and 21% (w/w phosphate resulted purification factors of 2.1 and 2.2-fold, recovering 100% and 73.5%, respectively of C-phycocyanin in the top phase.

  12. Partitioning of L-methionine in aqueous two-phase systems containing poly(propylene glycol) and sodium phosphate salts

    Energy Technology Data Exchange (ETDEWEB)

    Salabat, Alireza, E-mail: a-salabat@araku.ac.ir [Chemistry Department, Arak University, P.O. Box 38156-879, Arak (Iran, Islamic Republic of); Sadeghi, Rahmat [Department of Chemistry, University of Kurdistan, Sanandaj, Kurdistan 66135 (Iran, Islamic Republic of); Moghadam, Somayeh Tiani [Chemistry Department, Arak University, P.O. Box 38156-879, Arak (Iran, Islamic Republic of); Jamehbozorg, Bahman [Department of Chemistry, University of Kurdistan, Sanandaj, Kurdistan 66135 (Iran, Islamic Republic of)

    2011-10-15

    Highlights: > Thermodynamics parameters for partitioning of L-methionine in ATPS. > Investigation of different effects on partition coefficient of the amino acid. > Propose the best condition for L-methionine partitioning. - Abstract: The partitioning behavior of L-methionine has been studied in aqueous two-phase systems of (poly(propylene glycol) + sodium phosphate salts + H{sub 2}O) at different temperatures. The salts used were sodium di-hydrogen phosphate (NaH{sub 2}PO{sub 4}), di-sodium hydrogen phosphate (Na{sub 2}HPO{sub 4}) and tri-sodium phosphate (Na{sub 3}PO{sub 4}). The effects of tie line length, salt type, and temperature on the partition coefficient of this amino acid have been studied. In addition, thermodynamic parameters ({Delta}H{sup o}, {Delta}S{sup o} and {Delta}G{sup o}) as a function of temperature were calculated. The results showed that increasing tie line length led to decreasing of the partition coefficient. We also showed that the partition coefficients of the amino acid in the systems containing Na{sub 3}PO{sub 4} are greater than the other two salts. Moreover, it is verified that increasing temperature led to decreasing the partition coefficient. The experimental partition coefficient data are correlated using a modified virial-type model.

  13. Relative hydrophobicity between the phases and partition of cytochrome-c in glycine ionic liquids aqueous two-phase systems.

    Science.gov (United States)

    Wu, Changzeng; Wang, Jianji; Li, Zhiyong; Jing, Jun; Wang, Huiyong

    2013-08-30

    In this work, glycine ionic liquids tetramethylammonium glycine ([N1111][Gly]), tetraethylammonium glycine ([N2222][Gly]), tetra-n-butylammonium glycine ([N4444][Gly]), tetra-n-butylphosphonium glycine ([P4444][Gly]) and tetra-n-pentylammonium glycine ([N5555][Gly]) were synthesized and used to prepare aqueous two-phase systems (ATPSs) in the presence of K2HPO4. Binodal curves of such ATPSs and partition coefficients of a series of dinitrophenylated (DNP) amino acids in these ATPSs were determined at 298.15K to understand the effect of cationic structure of the ionic liquids on the phase-forming ability of glycine ionic liquids, relative hydrophobicity between the phases in the ionic liquids ATPSs, and polarity of the ionic liquids-rich phases. With the attempt to correlate the relative hydrophobicity of the phases in the ATPSs with their extraction capability for proteins, partition coefficients of cytochrome-c in the ATPSs were also determined. It was shown that partition coefficients of cytochrome-c were in the range from 2.83 to 20.7 under the studied pH conditions. Then, hydrophobic interactions between cytochrome-c and the ionic liquid are suggested to be the main driving force for the preferential partition of cytochrome-c in the glycine ionic liquid-rich phases of the ATPSs. Result derived from polarity of the ionic liquids-rich phases supports this mechanism.

  14. Development of green betaine-based deep eutectic solvent aqueous two-phase system for the extraction of protein.

    Science.gov (United States)

    Li, Na; Wang, Yuzhi; Xu, Kaijia; Huang, Yanhua; Wen, Qian; Ding, Xueqin

    2016-05-15

    Six kinds of new type of green betaine-based deep eutectic solvents (DESs) have been synthesized. Deep eutectic solvent aqueous two-phase systems (DES-ATPS) were established and successfully applied in the extraction of protein. Betaine-urea (Be-U) was selected as the suitable extractant. Single factor experiments were carried out to determine the optimum conditions of the extraction process, such as the salt concentration, the mass of DES, the separation time, the amount of protein, the temperature and the pH value. The extraction efficiency could achieve to 99.82% under the optimum conditions. Mixed sample and practical sample analysis were discussed. The back extraction experiment was implemented and the back extraction efficiency could reach to 32.66%. The precision experiment, repeatability experiment and stability experiment were investigated. UV-vis, FT-IR and circular dichroism (CD) spectra confirmed that the conformation of protein was not changed during the process of extraction. The mechanisms of extraction were researched by dynamic light scattering (DLS), the measurement of the conductivity and transmission electron microscopy (TEM). DES-protein aggregates and embraces phenomenon play considerable roles in the separation process. All of these results indicated that betaine-based DES-ATPS may provide a potential substitute new method for the separation of proteins.

  15. Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography.

    Science.gov (United States)

    Schwienheer, C; Prinz, A; Zeiner, T; Merz, J

    2015-10-01

    For the production of bio active compounds, e.g., active enzymes or antibodies, a conserved purification process with a minimum loss of active compounds is necessary. In centrifugal partition chromatography (CPC), the separation effect is based on the different distribution of the components to be separated between two immiscible liquid phases. Thereby, one liquid phase is kept stationary in chambers by a centrifugal field and the mobile phase is pumped through via connecting ducts. Aqueous two phase systems (ATPS) are known to provide benign conditions for biochemical products and seem to be promising when used in CPC for purification tasks. However, it is not known if active biochemical compounds can "survive" the conditions in a CPC where strong shear forces can occur due to the two-phasic flow under centrifugal forces. Therefore, this aspect has been faced within this study by the separation of active laccases from a fermentation broth of Pleurotus sapidus. After selecting a suitable ATPS and operating conditions, the activity yield was calculated and the preservation of the active enzymes could be observed. Therefore, CPC could be shown as potentially suitable for the purification of bio-active compounds.

  16. Salt effect on the (polyethylene glycol 8000 + sodium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Luisa A., E-mail: laferreira@deb.uminho.pt [IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga (Portugal); Teixeira, Jose A. [IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga (Portugal)

    2011-08-15

    Highlights: > Gibbs free energy of transfer of a methylene group on PEG 8000 - Na{sub 2}SO{sub 4} ATPS. > Influence of salt additive on the hydrophobic character of the coexisting phases. > Partitioning behavior of a series of five sodium salts of DNP-amino acids. > A relationship between {Delta}G(CH{sub 2}), TLL and I of the salt additive was established. - Abstract: The relative hydrophobicity of the phases of several {l_brace}polyethylene glycol (PEG) 8000 + sodium sulfate (Na{sub 2}SO{sub 4}){r_brace} aqueous two-phase systems (ATPSs), all containing 0.01 mol . L{sup -1} sodium phosphate buffer (NaPB, pH 7.4) and increasing concentration of a salt additive, NaCl or KCl, up to 1.0 mol . L{sup -1}, was measured by the free energy of transfer of a methylene group between the phases, {Delta}G(CH{sub 2}). The {Delta}G(CH{sub 2}) of the systems was determined by partitioning of a homologous series of five sodium salts of dinitrophenylated (DNP) - amino acids with aliphatic side chains in three different tie-lines of each biphasic system. The relative hydrophobicity of the phases ranged from -0.125 to -0.183 kcal . mol{sup -1}, being the NaCl salt the one to provide the more effective changes. The results show that, within each system, there is a linear relationship between the {Delta}G(CH{sub 2}) and the tie-line length (TLL), and biphasic systems with high salt additive concentration present the most negative {Delta}G(CH{sub 2}) values. Therefore, the feasibility of establishing a relationship between the relative hydrophobicity of the phases in a given TLL and the ionic strength of the salt additive was investigated and a satisfactory correlation was found for each salt.

  17. Extraction of natural red colorants from the fermented broth of Penicillium purpurogenum using aqueous two-phase polymer systems.

    Science.gov (United States)

    Santos-Ebinuma, Valéria Carvalho; Lopes, André Moreni; Pessoa, Adalberto; Teixeira, Maria Francisca Simas

    2015-01-01

    Safety concerns related to the increasing and widespread application of synthetic coloring agents have increased the demand for natural colorants. Fungi have been employed in the production of novel and safer colorants. In order to obtain the colorants from fermented broth, suitable extraction systems must be developed. Aqueous two-phase polymer systems (ATPPS) offer a favorable chemical environment and provide a promising alternative for extracting and solubilizing these molecules. The aim of this study was to investigate the partitioning of red colorants from the fermented broth of Penicillium purpurogenum using an ATPPS composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA). Red colorants partitioned preferentially to the top (PEG-rich phase). In systems composed of PEG 6,000 g/mol/NaPA 8,000 g/mol, optimum colorant partition coefficient (KC ) was obtained in the presence of NaCl 0.1 M (KC  = 10.30) while the PEG 10,000 g/mol/NaPA 8,000 g/mol system in the presence of Na2 SO4 0.5 M showed the highest KC (14.78). For both polymers, the mass balance (%MB) and yield in the PEG phase (%ηTOP ) were close to 100 and 79%, respectively. The protein selectivity in all conditions evaluated ranged from 2.0-3.0, which shows a suitable separation of the red colorants and proteins present in the fermented broth. The results suggest that the partitioning of the red colorants is dependent on both the PEG molecular size and salt type. Furthermore, the results obtained support the potential application of ATPPS as the first step of a purification process to recover colorants from fermented broth of microorganisms.

  18. Microcalorimetric Study on the Oscillating System of Two-phase Reaction of Aqueous Acid with Primary Amine in Chloroform

    Institute of Scientific and Technical Information of China (English)

    ZHANG,Hong-Lin; YU,Xiu-Fang; LU,Cheng-Xue; SUN,Si-Xiu; GUO,Guo-Hua; FU,Xun

    2003-01-01

    It has been found that the two-phase reactions of aqueous HCl,HOAc or H3PO4 with primary amine N1923 in chloroform are oscillating reactions. Their power-time curves were measured by the titration microcalorimetric method, and the induction period (tin).The first oscillating period (tp.1) and the second oscillating period(tp.2 ) were determined.The apparent activating parameters and the orders of the oscillating systems were calculated and the following relationships were established: for the oscillating system of hydrochloric acid tin∝c0.147HCIexp(1.35×103/T),.tp.1∝c0.241HCI·exp(4.33×103/T),tp.2∝c0.290HCIexp(5.59×103/T);for the oscillating system of acetic acid, tm∝c0.883HOAcexp(2.32×103/T),tp.1∝c0.399HOAc·exp(4.50×103/T),tp.2∝c0.301HOAcexp(5.88×103/T),for the oscillating system of phosphoric acid, tim∝c1.14H3PO4exp(7.70×104]T),tp.1∝c1.42H3PO4exp(1.14×104/T),tp.2∝c1.47H3PO4exp(1.27×104/T).

  19. Partitioning and purification of extracellular β-1,3-1,4-glucanase in aqueous two-phase systems

    Institute of Scientific and Technical Information of China (English)

    HE Guo-qing; ZHANG Xiu-yan; TANG Xing-jun; CHEN Qi-he; RUAN Hui

    2005-01-01

    The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4)was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on enzyme partition and extraction. The MW and concentration of PEG were found to have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly. pH had little effect on β-glucanase or proteases partition but affected α-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth.

  20. Determination of partition coefficients of biomolecules in a microfluidic aqueous two phase system platform using fluorescence microscopy.

    Science.gov (United States)

    Silva, D F C; Azevedo, A M; Fernandes, P; Chu, V; Conde, J P; Aires-Barros, M R

    2017-03-03

    Aqueous two phase systems (ATPS) offer great potential for selective separation of a wide range of biomolecules by exploring differences in molecular solubility in each of the two immiscible phases. However, ATPS use has been limited due to the difficulty in predicting the behavior of a given biomolecule in the partition environment together with the empirical and time-consuming techniques that are used for the determination of partition and extraction parameters. In this work, a fast and novel technique based on a microfluidic platform and using fluorescence microscopy was developed to determine the partition coefficients of biomolecules in different ATPS. This method consists of using a microfluidic device with a single microchannel and three inlets. In two of the inlets, solutions containing the ATPS forming components were loaded while the third inlet was fed with the FITC tagged biomolecule of interest prepared in milli-Q water. Using fluorescence microscopy, it was possible to follow the location of the FITC-tagged biomolecule and, by simply varying the pumping rates of the solutions, to quickly test a wide variety of ATPS compositions. The ATPS system is allowed 4min for stabilization and fluorescence micrographs are used to determine the partition coefficient.The partition coefficients obtained were shown to be consistent with results from macroscale ATPS partition. This process allows for faster screening of partition coefficients using only a few microliters of material for each ATPS composition and is amenable to automation. The partitioning behavior of several biomolecules with molecular weights (MW) ranging from 5.8 to 150kDa, and isoelectric points (pI) ranging from 4.7 to 6.4 was investigated, as well as the effect of the molecular weight of the polymer ATPS component.

  1. Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems

    Directory of Open Access Journals (Sweden)

    A. M. Lopes

    2014-12-01

    Full Text Available Aqueous two-phase micellar systems (ATPMS can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, which is influenced by the presence of electrolytes. In this work, we provide an investigation on the cloud point behavior of the nonionic surfactant C10E4 in the presence of NaCl, Li2SO4 and KI. We also investigated the salts' influence on a model protein partitioning. NaCl and Li2SO4 promoted a depression of the cloud point. The order of salts and the concentration that decreased the cloud point was: Li2SO4 0.5 M > NaCl 0.5 M ≈ Li2SO4 0.2 M. On the other hand, 0.5 M KI dislocated the curve to higher cloud point values. For our model protein, glucose-6-phosphate dehydrogenase (G6PD, partitioning experiments with 0.5 M NaCl or 0.2 M Li2SO4 at 13.85 ºC showed similar results, with K G6PD ~ 0.46. The lowest partition coefficient was obtained in the presence of 0.5 M KI (K G6PD = 0.12, with major recovery of the enzyme in the micelle-dilute phase (%Recovery = 90%. Our results show that choosing the correct salt to add to ATPMS may be useful to attain the desired partitioning conditions at more extreme temperatures. Furthermore, this system can be effective to separate a target biomolecule from fermented broth contaminants.

  2. Partition separation and characterization of the polyhydroxyalkanoates synthase produced from recombinant Escherichia coli using an aqueous two-phase system.

    Science.gov (United States)

    Lan, John Chi-Wei; Yeh, Chun-Yi; Wang, Chih-Chi; Yang, Yu-Hsuan; Wu, Ho-Shing

    2013-10-01

    Polyhydroxyalkanoates (PHAs) are renewable and biodegradable polyesters which can be synthesized either by numerous of microorganisms in vivo or synthase in vitro. The synthesis of PHAs in vitro requires an efficient separation for high yield of purified enzyme. The recombinant Escherichia coli harboring phaC gene derived from Ralstonia eutropha H16 was cultivated in the chemically defined medium for overexpression of synthase in the present work. The purification and characteristics of PHA synthase from clarified feedstock by using aqueous two-phase systems (ATPS) was investigated. The optimized concentration of ATPS for partitioning PHA synthase contained polyethylene glycol 6000 (30%, w/w) and potassium phosphate (8%, w/w) with 3.25 volume ratio in the absence of NaCl at pH 8.7 and 4°C. The results showed that the partition coefficient of enzyme activity and protein content are 6.07 and 0.22, respectively. The specific activity, selectivity, purification fold and recovery of phaC(Re) achieved 1.76 U mg⁻¹, 29.05, 16.23 and 95.32%, respectively. Several metal ions demonstrated a significant effect on activity of purified enzyme. The purified enzyme displayed maximum relative activity as operating condition at pH value of 7.5 and 37°C. As compared to conventional purification processes, ATPS can be a promising technique applied for rapid recovery of PHA synthase and preparation of large quantity of PHA synthase on synthesis of P(3HB) in vitro.

  3. Extraction and separation of tungsten (VI) from aqueous media with Triton X-100-ammonium sulfate-water aqueous two-phase system without any extractant.

    Science.gov (United States)

    Yongqiang Zhang; Tichang Sun; Tieqiang Lu; Chunhuan Yan

    2016-11-25

    An aqueous two-phase system composed of Triton X-100-(NH4)2SO4-H2O was proposed for extraction and separation of tungsten(VI) from aqueous solution without using any extractant. The effects of aqueous pH, concentration of ammonium sulfate, Triton X-100 and tungsten, extracting temperature on the extraction of tungsten were investigated. The extraction of tungsten has remarkable relationship with aqueous pH and are to above 90% at pH=1.0-3.0 under studied pH range (pH=1.0-7.0) and increases gradually with increasing Triton X-100 concentration, but decreases slightly with increasing ammonium sulfate concentration. The extraction percentage of tungsten is hardly relevant to temperature but its distribution coefficient linearly increases with increasing temperature within 303.15-343.15K. The distribution coefficient of tungsten increases with the increase of initial tungsten concentration (0.1-3%) and temperature (303.15 K-333.15K). The solubilization capacity of tungsten in Triton X-100 micellar phase is independent of temperature. FT-IR analysis reveals that there is no evident interaction between polytungstate anion and ether oxygen unit in Triton X-100, and DLS analysis indicates that zeta potential of Triton X-100 micellar phase have a little change from positive to negative after extracting tungsten. Based on the above-mentioned results, it can be deduced that polytungstate anions are solubilized in hydrophilic outer shell of Triton X-100 micelles by electrostatic attraction depending on its relatively high hydrophobic nature. The stripping of tungsten is mainly influenced by temperature and can be easily achieved to 95% in single stage stripping. The tungsten (VI) is separated out from solution containing Fe(III), Co(II), Ni(II), Cu(II), Zn(II), Al(III), Cr(III) and Mn(II) under the suitable conditions.

  4. Extraction of penicillin G by aqueous two-phase system of [Bmim]BF4/NaH2PO4

    Institute of Scientific and Technical Information of China (English)

    LIU Qingfen; HU Xuesheng; WANG Yuhong; YANG Ping; XIA Hansong; YU Jiang; LIU Huizhou

    2005-01-01

    A novel approach for the extraction of penicillin G by aqueous two-phase system comprised of hydrophilic ionic liquid [Bmim]BF4 (1-butyl-3- methylimidazolium tetrafluoroborate) and NaH2PO4 is reported. The effects of some important parameters involving the concentration of NaH2PO4, the concentration of penicillin G, the amount of [Bmim]BF4 on the formation of aqueous two-phase system and the extraction yield of penicillin were investigated. The primary result shows that the ATPS can take advantage of penicillin concentrated in upper phase at higher pH value for penicillin extraction from its aqueous solution without emulsification.

  5. Transformation of Vesicles in Aqueous Two-Phase System of an Anionic Gemini Surfactant and a Cationic Conventional Surfactant Mixture

    Institute of Scientific and Technical Information of China (English)

    JIANG Rong; HUANG Yi-Xiong; ZHAO Jian-Xi; HUANG Chang-Cang

    2008-01-01

    Transformation of vesicles formed in DTAB/C11-p-PhCNa aqueous surfactant two-phase (ASTP) was observed by the transmission electron microscopy (TEM). The trans-conformation of the gemini surfactant in the aggregates was considered to be the important factor for constructing the multi-lamellar structure of the vesicle wall. The cation-π interaction between the quaternary ammonium cation and the aromatic ring in the spacer was determined by the UV-Vis spectrum analysis, which, as well as the general electrostatic attraction and hydrophobic force, contributes to the stability of the multi-lamellar structure. The concentrations of the surface-active ions were measured for understanding the mechanism of vesicle transformation. The results show that isoelectric mixing of the two components benefits the growth of vesicles both in size and wall thickness.

  6. Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

    Directory of Open Access Journals (Sweden)

    Ana Lúcia Figueiredo Porto

    2004-09-01

    Full Text Available A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3 from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The best recovery of enzyme activity was 236%, with a purification factor of 34 in flow rates of 1 mL/min for dispersed phase.Uma coluna de discos perfurados rotativos foi utilizada na extração da enzima ascorbato oxidorredutase (E.C.1.10.3.3, obtida do extrato bruto de Curcubita maxima, através da utilização do sistema bifásico aquoso Polietilenoglicol-sais de fosfato. Os efeitos da velocidade da fase dispersa nos coeficientes de transferência de massa e na eficiência de separação para valores de 1, 2 e 3 mL/min foram estudados. Observou-se um aumento da transferência de massa com a velocidade da fase dispersa, enquanto que a eficiência de separação demonstrou uma ligeira redução com o aumento deste parâmetro. Os resultados experimentais obtidos durante a extração contínua demonstraram que a atividade da ascorbato oxidorredutase se concentrou preferencialmente na fase rica em sal para todas as condições estudadas. A maior recuperação da atividade enzimática foi de 236%, com um fator de purificação de 34 para o valor de 1 mL/min para a fase dispersa.

  7. Liquid-liquid equilibrium of water + PEG 8000 + magnesium sulfate or sodium sulfate aqueous two-phase systems at 35°C: experimental determination and thermodynamic modeling

    Directory of Open Access Journals (Sweden)

    B. D. Castro

    2005-09-01

    Full Text Available Liquid-liquid extraction using aqueous two-phase systems is a highly efficient technique for separation and purification of biomolecules due to the mild properties of both liquid phases. Reliable data on the phase behavior of these systems are essential for the design and operation of new separation processes; several authors reported phase diagrams for polymer-polymer systems, but data on polymer-salt systems are still relatively scarce. In this work, experimental liquid-liquid equilibrium data on water + polyethylene glycol 8000 + magnesium sulfate and water + polyethylene glycol 8000 + sodium sulfate aqueous two-phase systems were obtained at 35°C. Both equilibrium phases were analyzed by lyophilization and ashing. Experimental results were correlated with a mass-fraction-based NRTL activity coefficient model. New interaction parameters were estimated with the Simplex method. The mean deviations between the experimental and calculated compositions in both equilibrium phases is about 2%.

  8. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata) Seeds and Recycling of Phase Components.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Mohd Yazid; Hussin, Muhaini; Mustafa, Shuhaimi

    2015-06-17

    Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w) Triton X-100 and 20% (w/w) xylitol, at 56.2% of tie line length (TLL), (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases) and a crude load of 25% (w/w) at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  9. [Phase transfer catalyzed bioconversion of penicillin G to 6-APA by immobilized penicillin acylase in recyclable aqueous two-phase systems with light/pH sensitive copolymers].

    Science.gov (United States)

    Jin, Ke-ming; Cao, Xue-jun; Su, Jin; Ma, Li; Zhuang, Ying-ping; Chu, Ju; Zhang, Si-liang

    2008-03-01

    Immobilized penicillin acylase was used for bioconversion of penicillin PG into 6-APA in aqueous two-phase systems consisting of a light-sensitive polymer PNBC and a pH-sensitive polymer PADB. Partition coefficients of 6-APA was found to be about 5.78 in the presence of 1% NaCl. Enzyme kinetics showed that the reaction reached equilibrium at roughly 7 h. The 6-APA mole yields were 85.3% (pH 7.8, 20 degrees C), with about 20% increment as compared with the reaction of single aqueous phase buffer. The partition coefficient of PG (Na) varied scarcely, while that of the product, 6-APA and phenylacetic acid (PA) significantly varied due to Donnan effect of the phase systems and hydrophobicity of the products. The variation of the partition coefficients of the products also affected the bioconversion yield of the products. In the aqueous two-phase systems, the substrate, PG, the products of 6-APA and PA were biased in the top phase, while immobilized penicillin acylase at completely partitioned at the bottom. The substrate and PG entered the bottom phase, where it was catalyzed into 6-APA and PA and entered the top phase. Inhibition of the substrate and products was removed to result in improvement of the product yield, and the immobilized enzyme showed higher efficiency than the immobilized cells and occupied smaller volume. Compared with the free enzyme, immobilized enzyme had greater stability, longer life-time, and was completely partitioned in the bottom phase and recycle. Bioconversion in two-phase systems using immobilized penicillin acylase showed outstanding advantage. The light-sensitive copolymer forming aqueous two-phase systems could be recovered by laser radiation at 488 nm or filtered 450 nm light, while pH-sensitive polymer PADB could be recovered at the isoelectric point (pH 4.1). The recovery of the two copolymers was between 95% and 99%.

  10. A Novel Aqueous Two Phase System Composed of Surfactant and Xylitol for the Purification of Lipase from Pumpkin (Cucurbita moschata Seeds and Recycling of Phase Components

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2015-06-01

    Full Text Available Lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. A novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from Cucurbita moschata. The influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investigated. Moreover, the effect of system pH and crude load on the degree of purification and yield of the purified lipase were studied. The results indicated that the lipase was partitioned into the top surfactant rich phase while the impurities partitioned into the bottom xylitol-rich phase using an aqueous two phase system composed of 24% (w/w Triton X-100 and 20% (w/w xylitol, at 56.2% of tie line length (TLL, (TTL is one of the important parameters in this study and it is determined from a bimodal curve in which the tie-line connects two nodes on the bimodal, that represent concentration of phase components in the top and bottom phases and a crude load of 25% (w/w at pH 8.0. Recovery and recycling of components was also measured in each successive step process. The enzyme was successfully recovered by the proposed method with a high purification factor of 16.4 and yield of 97.4% while over 97% of the phase components were also recovered and recycled. This study demonstrated that the proposed novel aqueous two phase system method is more efficient and economical than the traditional aqueous two phase system method for the purification and recovery of the valuable enzyme lipase.

  11. Design and development of single stage purification of papain using Ionic Liquid based aqueous two phase extraction system and its Partition coefficient studies

    Directory of Open Access Journals (Sweden)

    Senthilkumar Rathnasamy

    2013-04-01

    Full Text Available As an emerging trend in bioseparation, aqueous two phase extractions based on phosponium ionic liquid have been utilized in this work to extract papain from Carica papaya fruit latex and the same wascompared with conventional aqueous two phase extraction system. Factors affecting the partition coefficient of papain such as ionic liquid concentration, pH of the extraction system and temperature have been investigated. The optimization studies show that ionic liquid concentrations and pH are majorly influencing the phaseformations and papain partitioning. It reveals the importance of electrostatic and hydrophobic interactions in the papain partitioning. Purification studies performed on Gel Filtration Chromatography shows that 96% of the papain enzyme could be extracted with the phosponium based ionic liquid in a single stage extraction. The final fraction containing papain enzyme was confirmed by SDS Page analysis.

  12. pH recycling aqueous two-phase systems applied in extraction of Maitake β-Glucan and mechanism analysis using low-field nuclear magnetic resonance.

    Science.gov (United States)

    Hou, Huiyun; Cao, Xuejun

    2015-07-31

    In this paper, a recycling aqueous two-phase systems (ATPS) based on two pH-response copolymers PADB and PMDM were used in purification of β-Glucan from Grifola frondosa. The main parameters, such as polymer concentration, type and concentration of salt, extraction temperature and pH, were investigated to optimize partition conditions. The results demonstrated that β-Glucan was extracted into PADB-rich phase, while impurities were extracted into PMDM-rich phase. In this 2.5% PADB/2.5% PMDM ATPS, 7.489 partition coefficient and 96.92% extraction recovery for β-Glucan were obtained in the presence of 30mmol/L KBr, at pH 8.20, 30°C. The phase-forming copolymers could be recycled by adjusting pH, with recoveries of over 96.0%. Furthermore, the partition mechanism of Maitake β-Glucan in PADB/PMDM aqueous two-phase systems was studied. Fourier transform infrared spectra, ForteBio Octet system and low-field nuclear magnetic resonance (LF-NMR) were introduced for elucidating the partition mechanism of β-Glucan. Especially, LF-NMR was firstly used in the mechanism analysis in partition of aqueous two-phase systems. The change of transverse relaxation time (T2) in ATPS could reflect the interaction between polymers and β-Glucan.

  13. Production of dry Lactobacillus rhamnosus GG preparations by spray drying and lyophilization in aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    Katarzyna Leja

    2009-12-01

    Full Text Available Background. Drying is the oldest method of food preservation. It works by removing water from the food, which prevents the growth of microorganisms and decay. Moreover, spray or freeze drying is also used for the preservation of probiotic cultures. The aim of this study was to compare a survival rate of probiotic bacteria Lactobacillus rhamnosus during spray and freeze drying in ATPS. These results were also compared with survival rate of cells dried under the same conditions but suspended only in skim milk, 6% solution of PVP or 6% solution of dextran. Material and methods. The bacteria Lactobacillus rhamnosus GGwere suspended and spray or freeze dried in various types of aqueous two-phase emulsions: PVP/dextran, PEG4000/dextran and PEG8000/dextran. These emulsions consisted of different types of polymers and had varying ratio of polymers in dispersed (dextran and dispersing (PEG and PVP phases. Results. The research demonstrated that survival rate of bacteria directly after drying depended mainly on protective reagent, rather than on drying method. After 30-day-storage of the dried bacteria cell specimens, the highest survival rate was noted in case of freeze dried cells in milk. In case of spray drying the highest cell survival rate was observed when emulsion PVP3.6%/dextran2.4% was used as a drying medium. Conclusions. Finally, it has been found that cell survival rate was not strongly influenced by the storage temperature of the powder but it depended on the drying medium.  

  14. Partitioning of Cephalexin in Ionic Liquid Aqueous Two-Phase System Composed of 1-Butyl-3-Methylimidazolium Tetrafluoroborate and ZnSO4

    Directory of Open Access Journals (Sweden)

    Yan Fang Li

    2013-01-01

    Full Text Available Ionic liquid aqueous two-phase system (ILATPS was applied in the extraction and separation of hydrosoluble antibiotics. The partitioning behavior of cephalexin (CEX in 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4-ZnSO4 aqueous two-phase system was studied by the partitioning parameter of the extraction efficiency. The effect of the volume of [Bmim]BF4, the concentration of ZnSO4, temperature, pH, and the volume of ZnSO4 solution was discussed concretely. When the volume of [Bmim]BF4 was 2 mL and the concentration of ZnSO4 was 35%, the extraction efficiency of CEX could reach 92.64% with pH unadjusted. The effect of the volume of [Bmim]BF4 on the extraction efficiency was higher than that of the concentration of ZnSO4. The temperature influenced not only the formation of aqueous two-phase system but also the extraction efficiency of CEX. The target was found to be preferentially extracted to the [Bmim]BF4-rich phase at the pH below 4.3. The partition of CEX to the top phase was enhanced by increasing the volume of [Bmim]BF4, the concentration of ZnSO4, and temperature; however, the partition of CEX to the top phase increased by decreasing the pH.

  15. Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds.

    Science.gov (United States)

    Liu, Dan; Hong, Zhilai; Gao, Mingzhe; Wang, Zhixin; Gu, Ming; Zhang, Xiaozhe; Xiao, Hongbin

    2016-05-27

    The essential attributes of a solvent system for separation polar compounds on CCC are polarity, selectively and performance. Here, hydrophilic organic/salt-containing aqueous two-phase system (HO/S TPS) was evaluated as an alternative solvent system for CCC separation of polar compounds. Polarity measurements based on Rohrschneider-Snyder parameter was developed as quantitative assessing the polarity of HO/S TPS and comparing with an organic/aqueous system. All investigated 1-butanol/ethanol/saturated ammonium sulfate solution/water (BEAsWat) and 1-butanol/ethanol/saturated dipotassium hydrogen phosphate solution/water (BEDhpWat) systems with polarity values of organic phase from 4.5 to 6.8, were more polar than chloroform/methanol/water (1/1/1). The considerable water content of BEAsWat and BEDhpWat (0/1/1/1/) was 45.4 and 42.6% (w%) of hydrophilic organic phase, and 66.4 and 51.2% (w%) of salt-containing aqueous phase, respectively, closed to conventional aqueous two-phase system. Therefore, the polarity of HO/S TPS is in the middle of organic/aqueous and aqueous two-phase system. The LogKC values of twenty four polar compounds as model mixture confirmed that the polarities of HO/S TPSs were matched to that of the polar compounds and shown to be a very selective technique capable of separating positional isomers. Moreover, BEAsWat and BEDhpWat systems can be easily retained in CCC column with suitable elution mode. The hydrodynamic behavior reversion of HO/S TPS on hydrodynamic CCC was observed and was tentatively explained based on the density difference. Finally, caffeoylquinic acid isomers and dihydroxybenzoic acid isomers were successfully separated with HO/S TPS on CCC, respectively. Those results demonstrate that HO/S TPS on CCC is a performant and stable way to separate polar compounds from natural products.

  16. Chelate-Modified Fenton Reaction for the Degradation of Trichloroethylene in Aqueous and Two-Phase Systems

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, Scott [Univ of KY, dept of chemical and materials engineering; lynch, Andrew [Univ of KY, dept of chemical and materials engineering; Bachas, Leonidas [Univ of KY, Dept of Chemistry; hampson, Steve [Univ of KY Center for Applied Energy Research - KY Research Consortium of Energy and Environment; Ormsbee, Lindelle [Univ of KY Center for Applied Energy Research - KY Research Consortium of Energy and Environment; Bhattacharyya, Dibakar [Univ of KY, dept of chemical and materials engineering

    2008-06-01

    The Standard Fenton reaction has been used for In-Situ Chemical Oxidation (ISCO) of toxic organics in groundwater. However, it requires low pH operating conditions, and thus has limitations for in situ applications. In addition, hydroxyl radicals are rapidly consumed by hydroxyl scavengers found in the subsurface. These problems are alleviated through the chelate-modified Fenton (hydroxyl radical) reaction, which includes the addition of nontoxic chelate (L) such as citrate or gluconic acid. This chelate allows the reaction to take place at bear neutral pH and control hydrogen peroxide consumption by binding to Fe(II), forming an FeL complex. The chelate also binds to Fe(III), preventing its precipitation as ferric hydroxide and thus prevents problems associated with injection well plugging. The rate of TCE dechlorination in chelate-modified Fenton systems is a function of pH, H2O2 concentration, and FE:L ratio. The primary objective of this research is to model and apply this process to the destruction of trichloroethylene (TCE) present in both the aqueous and organic (in the form of droplets) phases. Experimentation proved the chelate-modified Fenton reaction effectively dechlorinates TCE in both the aqueous and organic phases at near-neutral pH. Other focuses of this work include determining the effect of [L]:[Fe] ratios on H2O2 and TCE degradation as well as reusability of the FE citrate solution under repeated H2O2 injections. Generalized models were developed to predict the concentration of TCE in the aqueous phase and TCE droplet radius as a function of time using established hydroxyl radial kinetics and mass transfer relationships.

  17. Dependence of chymosin and pepsin partition coefficient with phase volume and polymer pausidispersity in polyethyleneglycol-phosphate aqueous two-phase system.

    Science.gov (United States)

    Spelzini, Darío; Picó, Guillemo; Farruggia, Beatriz

    2006-08-01

    The influence of the phase volume ratio and polymer pausidispersity on chymosin and pepsin partition in polyethylenglycol-phosphate aqueous two-phase systems was studied. Both proteins showed a high affinity for the polyethylenglycol rich phase with a partition coefficient from 20 to 100 for chymosin and from 20 to 180 for pepsin, when the polyethyleneglycol molecular mass in the system varied between 1450 and 8000. The partition coefficient of chymosin was not affected by the volume phase ratio, while the pepsin coefficient showed a significant decrease in its partition coefficient with the increase in the top/bottom phase volume ratio.

  18. Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems

    OpenAIRE

    Tubío, Gisela; Venâncio, Armando; Teixeira, J. A.; Nerli, Bibiana B.; Picó, Guillermo A.

    2008-01-01

    The phase diagrams of Ucon 50-HB-5100, a non-ionic random copolymer of ethylene oxide and propylene oxide (EOPO) and sodium citrate aqueous two-phase systems were determined at different pHs (5.20 and 8.20) and temperatures (5, 20 and 40º C). The binodal curves were determined by refractive index and enzymatic assay of the solution and described using a four-parameter sigmoidal equation, the reliability of the measured tie line compositions was ascertained by correlation equations...

  19. Lactose hydrolysis in aqueous two-phase system by whole-cell {beta}-galactosidase of Kluyveromyces marxianus. Semicontinuous and continuous processes

    Energy Technology Data Exchange (ETDEWEB)

    Tomaska, M. [Slovak Technical Univ., Bratislava (Slovakia). Dept. of Biochemical Technology; Stredansky, M. [Slovak Technical Univ., Bratislava (Slovakia). Dept. of Biochemical Technology; Tomaskova, A. [Slovak Technical Univ., Bratislava (Slovakia). Dept. of Biochemical Technology; Sturdik, E. [Slovak Technical Univ., Bratislava (Slovakia). Dept. of Biochemical Technology

    1995-01-01

    Semicontinuous and continuous hydrolysis of lactose in aqueous two-phase systems (polyethylene glycol 20000/ dextran 40) with whole-cell {beta}-galactosidase of K. marxianus were studied. Both phase polymers had no effect on {beta}-galactosidase activity confined in cells. Good operational stability of the biocatalyst during 55 cycles of semicontinuous process was observed without appreciable decrease in product concentration. Continuous hydrolysis of lactose was performed in the stirred bioreactor, connected with the phase separator. The satisfactory degree of hydrolysis (between 82-88%) and volumetric productivity (21.6 g/l/h) were reached during 72 hours of continuous hydrolysis of 5% (w/w) lactose. (orig.)

  20. A microdevice assisted approach for the preparation, characterization and selection of continuous aqueous two-phase systems: from micro to bench-scale.

    Science.gov (United States)

    Vázquez-Villegas, Patricia; Ouellet, Eric; González, Claudia; Ruiz-Ruiz, Federico; Rito-Palomares, Marco; Haynes, Charles A; Aguilar, Oscar

    2016-07-05

    Aqueous two-phase systems (ATPS) have emerged as an alternative strategy for the recovery and purification of a wide variety of biological products. Typical process development requires a large screening of experimental conditions towards industrial adoption where continuous processes are preferred. In this work, it was proved that under certain flow conditions, ATPS could be formed continuously inside a microchannel, starting from stocks of phase components. Staggered herringbone chaotic micromixers included within the device sequentially and rapidly prepare two-phase systems across an entire range of useful phase compositions. Two-phase diagrams (binodal curves) were easily plotted using the cloud-point method for systems of different components and compared with previously reported curves for each system, proving that phase formation inside the device correlated with the previously reported diagrams. A proof of concept for sample partitioning in such a microdevice was performed with two different experimental models: BSA and red blood cells. Finally, the microdevice was employed to obtain information about the recovery and partition coefficient of invertase from a real complex mixture of proteins (yeast extract) to design a process for the recovery of the enzyme selecting a suitable system and composition to perform the process at bench-scale.

  1. Separation of clavulanic acid from fermented broth of amino acids by an aqueous two-phase system and ion-exchange adsorption.

    Science.gov (United States)

    da Silva, Clovis Sacardo; Cuel, Maressa Fabiano; Barreto, Verônica Orlandin; Kwong, Wu Hong; Hokka, Carlos O; Barboza, M

    2012-02-15

    The clavulanic acid is a substance which inhibits the β-lactamases used with penicillins for therapeutic treatment. After the fermentation, by-products of low molecular weight such as amino acids lysine, histidine, proline and tyrosine are present in the fermented broth. To remove these impurities the techniques of extraction by an aqueous two-phase system of 17% polyethylene glycol molecular weight 600 and 15% potassium phosphate were used for a partial purification. A subsequent ion-exchange adsorption was used for the recuperation of the clavulanic acid of the top phase and purification getting a concentration factor of 2 and purification of 100% in relation to the amino acids lysine, histidine, proline and tyrosine.

  2. An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

    Directory of Open Access Journals (Sweden)

    GN Gómez

    2012-01-01

    Full Text Available Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the homogeneity of the isolated proteins.

  3. Determination of sunset yellow and tartrazine in food samples by combining ionic liquid-based aqueous two-phase system with high performance liquid chromatography.

    Science.gov (United States)

    Sha, Ou; Zhu, Xiashi; Feng, Yanli; Ma, Weixing

    2014-01-01

    We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs) with high performance liquid chromatography (HPLC), for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

  4. Determination of Sunset Yellow and Tartrazine in Food Samples by Combining Ionic Liquid-Based Aqueous Two-Phase System with High Performance Liquid Chromatography

    Directory of Open Access Journals (Sweden)

    Ou Sha

    2014-01-01

    Full Text Available We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs with high performance liquid chromatography (HPLC, for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01–50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

  5. The tendency of smooth and rough Salmonella typhimurium bacteria and lipopolysaccharide to hydrophobic and ionic interaction, as studied in aqueous polymer two-phase systems.

    Science.gov (United States)

    Magnusson, K E; Stendahl, O; Tagesson, C; Edebo, L; Johansson, G

    1977-06-01

    In aqueous two-phase system, the partition of bacteria and lipopolysaccharide from a rough (R) strain (Rd-mutant) of Salmonella typhimurium is influenced by polymers with covalently linked hydrophobic groups indicating hydrophobic structures accessible at the cell surface. Furthermore, the partition of the R bacteria is influenced by a number of inorganic positive and negative ions, presumably as a consequence of interaction with negatively charged surface structures. In contrast, smooth (S) bacteria and lipopolysaccharide from the parent strain do not seem to participate in either hydrophobic or charge interaction indicating extensive hydrophilicity without charge. Thus, the S-specific polysaccharide side chain of S. typhimurium might serve the purpose of blindfolding aspecific host defence mechanisms dependent on hydrophobicity and charge. On the contrary, the R bacteria and R lipopolysaccharide have physico-chemical properties which predispose to interaction with several types of cells, organelles and molecules.

  6. Optimization of ultrasound-assisted aqueous two-phase system extraction of polyphenolic compounds from Aronia melanocarpa pomace by response surface methodology.

    Science.gov (United States)

    Xu, Yan-Yang; Qiu, Yang; Ren, Hui; Ju, Dong-Hu; Jia, Hong-Lei

    2016-10-13

    Aronia melanocarpa berries are abundant in polyphenolic compounds. After juice production, the pomace of pressed berries still contains a substantial amount of polyphenolic compounds. For efficient utilization of A. melanocarpa berries and the enhancement of polyphenolic compound yields in Aronia melanocarpa pomace (AMP), total phenolics (TP) and total flavonoids (TF) from AMP were extracted, using ultrasound-assisted aqueous two-phase system (UAE-ATPS) extraction method. First, the influences of ammonium sulfate concentration, ethanol-water ratio, ultrasonic time, and ultrasonic power on TP and TF yields were investigated. On this basis, process variables such as ammonium sulfate concentration (0.30-0.35 g mL(-1)), ethanol-water ratio (0.6-0.8), ultrasonic time (40-60 min), and ultrasonic power (175-225 W) were further optimized by implementing Box-Benhnken design with response surface methodology. The experimental results showed that optimal extraction conditions of TP from AMP were as follows: ammonium sulfate concentration of 0.324 g mL(-1), ethanol-water ratio of 0.69, ultrasonic time of 52 min, and ultrasonic power of 200 W. Meanwhile, ammonium sulfate concentration of 0.320 g mL(-1), ethanol-water ratio of 0.71, ultrasonic time of 50 min, and ultrasonic power of 200 W were determined as optimum extraction conditions of TF in AMP. Experimental validation was performed, where TP and TF yields reached 68.15 ± 1.04 and 11.67 ± 0.63 mg g(-1), respectively. Close agreement was found between experimental and predicted values. Overall, the present results demonstrated that ultrasound-assisted aqueous two-phase system extraction method was successfully used to extract total phenolics and flavonoids in A. melanocarpa pomace.

  7. Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants

    Institute of Scientific and Technical Information of China (English)

    ZHANG Junwei; WANG Yan; PENG Qijun

    2013-01-01

    Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactant two-phase system (ASTP) with cationic suffactant (CTAB) and anionic surfactant (SDS).Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant.The increase in concentrations of NaBr and Na2SO4 are beneficial to the formation of ASTP.Theanine concentration in the bottom phase increases with increasing concentration of theanine,whereas the partition coefficient and extraction rate only change a little when the concentration of theanine is above 0.2 g· L-1.With the increase of SDS concentration,the phase ratio and the partition coefficient decrease,while the extraction efficiency of theanine increases and the concentration of theaninc changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio.The temperature has a notable effect on the concentration of theanine in the bottom phase,partition coefficient and extraction rate of theanine.The increase of waste liquid decreases the phase ratio,increases the concentration and extraction rate of theanine in the bottom phase,since the protein and the saccharide enter the bottom phase with theanine.

  8. Separation of curcuminoids using ionic liquid based aqueous two-phase system coupled with in situ dispersive liquid-liquid microextraction.

    Science.gov (United States)

    Shu, Yang; Gao, Mingcen; Wang, Xueying; Song, Rusheng; Lu, Jun; Chen, Xuwei

    2016-01-01

    An aqueous two-phase extraction system (ATPS) combined with an in situ dispersive liquid-liquid microextraction (DLLME) method using imidazolium ionic liquids (ILs) for the separation of curcuminoids is developed. The influence of structure of IL, the type of metathesis reagents, and the back extraction agents on the extraction efficiency is investigated. 2.0mg of curcuminoids are extracted by an IL ATPS composed of 0.4g 1,3-diethylimidazolium iodine (EeimI), 0.6g potassium hydrogen phosphate, 1.0g water. Then the bis[(trifluoromethyl)sulfonyl]imide lithium (LiNTf2) aqueous solution is added to the EeimI-rich phase of the ATPS. The water-immiscible ionic liquids, 1,3-diethylimidazole bis[(trifluoromethyl)sulfonyl]imide (EeimNTf2), forms by the metathesis reaction. The in situ DLLME is triggered simultaneously and further purifies the curcuminoids. 92% of EeimI transforms into EeimNTf2 and thus the Eeim(+) cation is used for twice in this method. Finally, 0.1mol/L NaOH aqueous solution is used as the back extraction reagent. The curcuminoids precipitate is achieved with 93% of recovery when the aqueous solution is adjusted to pH 3.0. This ATPS-DLLME method is successfully applied to the separation of curcuminoids from Curcuma Longa (0.96±0.02% of extraction yield, a purity of >51% with respect to the total dry mass of the product).

  9. Prediction of liquid-liquid equilibria for polyethylene glycol based aqueous two-phase system by ASOG and UNIFAC method

    Directory of Open Access Journals (Sweden)

    M. Perumalsamy

    2009-03-01

    Full Text Available Liquid-Liquid equilibrium data were obtained for the polyethylene glycol2000(PEG2000-sodium citrate-water system at 298.15, 308.15 and 318.15 K. The effect of temperature on binodal and tie line data was studied and published in a previous article (Murugesan and Perumalsamy, 2005. The interaction parameters of ASOG and UNIFAC models were estimated using the LLE data of PEG2000-sodium citrate-water system and are used to predict the LLE data for PEG6000-sodium citrate-water system at 298.15, 308.15 and 318.15 K (literature data. The predicted LLE data by both ASOG and UNIFAC models showed good agreement with the experimental and literature data.

  10. (Liquid + liquid) and (liquid + solid) equilibrium of aqueous two-phase systems containing poly ethylene glycol di-methyl ether 2000 and di-sodium hydrogen phosphate

    Energy Technology Data Exchange (ETDEWEB)

    Zafarani-Moattar, Mohammed Taghi, E-mail: zafarani47@yahoo.co [Physical Chemistry Department, University of Tabriz, Tabriz 51664 (Iran, Islamic Republic of); Nasiri, Saeed [Physical Chemistry Department, University of Tabriz, Tabriz 51664 (Iran, Islamic Republic of)

    2010-09-15

    (Liquid + liquid) equilibrium (LLE) for the {l_brace}poly ethylene glycol di-methyl ether 2000 (PEGDME{sub 2000}) + Na{sub 2}HPO{sub 4} + H{sub 2}O{r_brace} system have been determined experimentally at T = (298.15, 308.15, 313.15, and 318.15) K. The effects of temperature on the binodals and tie-lines for the investigated aqueous two-phase system (ATPS) have been studied. An empirical non-linear expression developed by Merchuk was used for reproducing the experimental binodal data. In this work, the three fitting parameters of the Merchuk equation were obtained with the temperature dependence expressed in the linear form with (T - T{sub 0}) K as a variable. Furthermore, the modified local composition segment-based NRTL and Wilson models and also osmotic virial equation were used to describe the LLE data of the studied system. Also, the effects of the type of salt on LLE are discussed. In addition, the effects of end groups of the polymers PEGDME{sub 2000} and poly ethylene glycol 2000 on phase forming ability were studied. The complete phase diagram for the poly ethylene {l_brace}glycol di-methyl ether 2000 (PEGDME{sub 2000}) + Na{sub 2}HPO{sub 4} + H{sub 2}O{r_brace} system has also been determined at T = 298.15 K.

  11. 手性药物扁桃酸的双水相分离%Study on partition of D(-)-mandelic acid in aqueous two-phase systems

    Institute of Scientific and Technical Information of China (English)

    许小平; 李忠琴; 陈杰波

    2004-01-01

    Studies on the partition of D (-)-mandelic acid were performed in polyethylene glycol (PEG) with molecular masses of 2000, 4000, 6000, 10000, 20000- ammonium sulfate systems. The effect of various parameters such as molecular mass of PEG, phase composition, pH of the system and temperature were investigated in this work. The results indicate that the molecular mass of PEG is a key factor affecting the partitioning behaviors of D(-)-mandelic acid in the PEG -(NH4)2SO4 systems. The influence of pH on the partition shows that the partition coefficient of D(-)-mandelic acid decreases with the increase of pH value. D(-)-mandelic acid partition is only slightly influenced by the temperature. All these indicate that the aqueous two-phase is a very suitable alternative system for the separation of D(-)-mandelic acid.%采用聚乙二醇-硫酸铵双水相系统分离手性药物扁桃酸.研究了聚乙二醇分子量、相组成、pH值和温度对扁桃酸分离的影响.结果表明,聚乙二醇分子量对双水相分离扁桃酸的影响最大;温度对扁桃酸的分离几乎没有影响;随pH增大,扁桃酸分配系数减少.

  12. Direct Purification of Pectinase from Mango (Mangifera Indica Cv. Chokanan Peel Using a PEG/Salt-Based Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2011-10-01

    Full Text Available An Aqueous Two-Phase System (ATPS was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000, potassium phosphate composition (12–20%, w/w, system pH (6–9, and addition of different concentrations of neutral salts (0–8%, w/w on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%. Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  13. Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

    Science.gov (United States)

    Mehrnoush, Amid; Sarker, Md Zaidul Islam; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2011-10-10

    An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

  14. Aqueous two-phase system cold-set gelation using natural and recombinant probiotic lactic acid bacteria as a gelling agent.

    Science.gov (United States)

    Léonard, Lucie; Husson, Florence; Langella, Philippe; Châtel, Jean-Marc; Saurel, Rémi

    2016-05-01

    The present study aimed to entrap probiotic lactic acid bacteria (LAB) in a sodium alginate and sodium caseinate aqueous two-phase gel system. The natural acidifying properties of two therapeutic probiotic LAB were exploited to liberate calcium ions progressively from calcium carbonate (CaCO3), which caused the gelation of the co-existing phases. Bi-biopolymeric matrix gelation of GDL/CaCO3 or LAB/CaCO3 was monitored by dynamic rheological measurements, and the final gels were characterized by frequency dependence measurements and confocal laser scanning microscopy. Weak to strong gels were formed with an elastic modulus G' from 10 to 1.000Pa, respectively. After cold-set gelation of our system, confocal laser scanning microscopy showed spherical protein microdomains trapped within a calcium alginate network. LAB cells were stained to study their partition in the self-gelling matrices. Our LAB strains showed two different behaviors, which may relate to the exopolysaccharide production: (i) Lactobacillus plantarum CNRZ1997 cells were found mainly in continuous alginate networks, whereas (ii) Lactococcus lactis cells were localized in protein microdomains. This alginate-caseinate phase-separated system that was self-gelled by LAB cells may be an innovative approach for immobilizing and protecting LAB cells.

  15. Optimization of serine protease purification from mango (Mangifera indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system.

    Science.gov (United States)

    Mehrnoush, Amid; Mustafa, Shuhaimi; Sarker, Md Zaidul Islam; Yazid, Abdul Manap Mohd

    2012-01-01

    Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g·mol(-1)), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g·mol(-1) of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  16. A novel aqueous micellar two-phase system composed of surfactant and sorbitol for purification of pectinase enzyme from Psidium guajava and recycling phase components.

    Science.gov (United States)

    Amid, Mehrnoush; Murshid, Fara Syazana; Manap, Mohd Yazid; Hussin, Muhaini

    2015-01-01

    A novel aqueous two-phase system composed of a surfactant and sorbitol was employed for the first time to purify pectinase from Psidium guajava. The influences of different parameters, including the type and concentration of the surfactant and the concentration and composition of the surfactant/sorbitol ratio, on the partitioning behavior and recovery of pectinase were investigated. Moreover, the effects of system pH and the crude load on purification fold and the yield of purified pectinase were studied. The experimental results indicated that the pectinase was partitioned into surfactant-rich top phase, and the impurities were partitioned into the sorbitol-rich bottom phase with the novel method involving an ATPS composed of 26% (w/w) Triton X-100 and 23% (w/w) sorbitol at 54.2% of the TLL crude load of 20% (w/w) at pH 6.0. The enzyme was successfully recovered by this method with a high purification factor of 15.2 and a yield of 98.3%, whereas the phase components were also recovered and recycled at rates above 96%. This study demonstrated that this novel ATPS method can be used as an efficient and economical alternative to the traditional ATPS for the purification and recovery of the valuable enzyme.

  17. Potential application of aqueous two-phase systems and three-phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of Kluyveromyces marxianus.

    Science.gov (United States)

    Simental-Martínez, Jesús; Rito-Palomares, Marco; Benavides, Jorge

    2014-01-01

    Superoxide dismutase (SOD; EC 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. In the present work, the partition behavior of SOD in aqueous two-phase systems (ATPS) (using a standard solution and a complex extract from Kluyveromyces marxianus as sample) was characterized on different types of ATPS (polymer-polymer, polymer-salt, alcohol-salt, and ionic liquid (IL)-salt). The systems composed of PEG 3350-potassium phosphate, 45% TLL, 0.5 M NaCl (315 U/mg, 87% recovery, and 15.1-fold purification) and t-butanol-20% ammonium sulfate (205.8 U/mg, 80% recovery and 9.8-fold purification), coupled with a subsequent 100 kDa ultrafiltration stage, allowed the design of a prototype process for the recovery and partial purification of the product of interest. The findings reported herein demonstrate the potential of PEG-salt ATPS for the potential recovery of SOD.

  18. Water-head-driven microfluidic oscillators for autonomous control of periodic flows and generation of aqueous two-phase system droplets.

    Science.gov (United States)

    Dang, Van Bac; Kim, Sung-Jin

    2017-01-17

    Generating periodic flows with an oscillator driven only by water-head pressure has potential for the operation of microfluidic systems without any dynamic off-chip controllers. However, its operational characteristic is not well understood due to complex dynamic interactions of the microfluidic components. Here, we focus on the mechanism of a water-head-driven oscillator and analyze the functions of its flow-switching period (T) and flow rate (Q) in a wide range (0.1 s-5.9 h and 2 μL min(-1)-2 mL min(-1)). We show linear control of T and Q by their corresponding fluidic resistors even with the complex and nonlinear relation of the microfluidic components. This allows independent regulation of T and Q within their operational ranges but we found the two parameters mutually constrain their ranges via fluidic resistance. Also, we characterize the control of T by water-head pressure and present operational ranges of input water-head pressure decrease with increasing output water-head pressure. To show its utility, we apply the oscillator to generate droplets with low interfacial tension aqueous two-phase systems. Our study would be useful and provide the foundation for various functions of water-head-driven microfluidic circuits.

  19. Extraction protease expressed by Penicillium fellutanum from the Brazilian savanna using poly(ethylene glycol)/sodium polyacrylate/NaCl aqueous two-phase system.

    Science.gov (United States)

    Barros, Kleber V G; Souza, Paula M; Cardoso, Samuel L; Borges, Leonardo L; Filho, Edivaldo X F; Junior, Adalberto P; Magalhães, Pérola O

    2015-01-01

    The partitioning of protease expressed by Penicillium fellutanum from the Brazilian savanna in a novel inexpensive and stable aqueous two-phase system (ATPS) composed of poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) was studied in this work using factorial design. The ATPS is formed by mixing both polymers with a salt (NaCl) and fermented broth of P. fellutanum. The effects of molar mass (2,000, 4,000, and 6,000 g ⋅ mol(-1)) and concentration (6, 8, and 10 wt%) of PEG and that of NaPA concentration (6, 8, and 10 wt%) on protease partitioning (K) at 25 °C were studied. A two-level factorial design (2(3)) was implemented. The effect of Na2 SO4 concentration (5, 10, and 15 wt%) on the reextraction of the enzyme was also analyzed. The partition coefficient K ranged from 77.51 to 1.21, indicating the versatility of the method. The reextraction was achieved with the addition of 5% Na2 SO4 , allowing the partitioning of the protease to the upper phase, whereas total proteins were directed to the bottom phase. The results of partitioning using the PEG/NaPA/NaCl system and that of the subsequent reextraction with Na2 SO4 suggest that this method can be used to purify proteases from fermented broth of P. fellutanum.

  20. Ionic liquid-based ultrasound-assisted extraction and aqueous two-phase system for analysis of caffeoylquinic acids from Flos Lonicerae Japonicae.

    Science.gov (United States)

    Tan, Ting; Lai, Chang-Jiang-Sheng; OuYang, Hui; He, Ming-Zhen; Feng, Yulin

    2016-02-20

    In this work, an ionic liquid-based ultrasonic-assisted extraction (ILUAE) method was developed to extract caffeoylquinic acids (CQAs) from Flos Lonicerae Japonicae (FLJ). ILUAE parameters were optimized by response surface methodology, including IL concentration, ultrasonic time, and liquid-solid ratio. Optimized ILUAE approach gained the highest extraction yields of 28.53, 18.21, 3.84mg/g for 3-O-caffeoylquinic acid (C1), 3,5-di-O-caffeoylquinic acid (C2), 3,4-di-O-caffeoylquinic acid (C3), respectively. C1-C3 are the three most abundant CQAs compounds in FLJ. The method showed comparable extraction yield and shorter extraction time compared with conventional extraction techniques. Subsequently, an aqueous two-phase system (ATPS) was applied in extraction solutions. Two trace CQAs, 5-O-caffeoylquinic acid (C4) and 4,5-di-O-caffeoylquinic acid (C5), were significantly enriched with signal to noise values increasing from less than 10 to higher than 1475. The results indicated that ILUAE and ATPS are efficient and environmentally-friendly sample extraction and enrichment techniques for CQAs from herbal medicines.

  1. Interrelationship between partition behavior of organic compounds and proteins in aqueous dextran-polyethylene glycol and polyethylene glycol-sodium sulfate two-phase systems.

    Science.gov (United States)

    Ferreira, Luisa A; da Silva, Nuno R; Wlodarczyk, Samarina R; Loureiro, Joana A; Madeira, Pedro P; Teixeira, José A; Uversky, Vladimir N; Zaslavsky, Boris Y

    2016-04-22

    Partition behavior of adenosine and guanine mononucleotides was examined in aqueous dextran-polyethylene glycol (PEG) and PEG-sodium sulfate two-phase systems. The partition coefficients for each series of mononucleotides were analyzed as a functions of the number of phosphate groups and found to be dependent on the nature of nucleic base and on the type of ATPS utilized. It was concluded that an average contribution of a phosphate group into logarithm of partition coefficient of a mononucleotide cannot be used to estimate the difference between the electrostatic properties of the coexisting phases of ATPS. The data obtained in this study were considered together with those for other organic compounds and proteins reported previously, and the linear interrelationship between logarithms of partition coefficients in dextran-PEG, PEG-Na2SO4 and PEG-Na2SO4-0.215M NaCl (all in 0.01M Na- or K/Na-phosphate buffer, pH 7.4 or 6.8) was established. Similar relationship was found for the previously reported data for proteins in Dex-PEG, PEG-600-Na2SO4, and PEG-8000-Na2SO4 ATPS. It is suggested that the linear relationships of the kind established in ATPS may be observed for biological properties of compounds as well.

  2. [Isolation and purification of alpha-glycerophosphate oxidase in a polyethylene glycol/(NH4 )2SO4 aqueous two-phase system].

    Science.gov (United States)

    Meng, Yao; Jin, Jiagui; Liu, Shuangfeng; Yang, Min; Zhang, Qinglian; Wan, Li; Tang, Kun

    2014-02-01

    Alpha-glycerophosphate oxidase (alpha-GPO) from Enterococcus casseli flavus was successfully isolated and purified by using polyethylene glycol (PEG)/(NH4)2SO4 aqueous two-phase system (ATPS). The results showed that the chosen PEG/(NH4)2SO4 ATPS could be affected by PEG molecular weight, pH, concentration of PEG and (NH4)2SO4, and inorganic salt as well as additional amount of crude enzyme. After evaluating these influencing factors, the final optimum purification strategy was formed by 16.5% (m/m) PEG2000, 13.2% (m/m) (NH4)2SO4, pH 7.5 and 30% (m/m) additive crude enzyme, respectively. The NaCl was a negative influencing factor which would lead to lower purification fold and activity recovery. These conditions eventually resulted in the activity recovery of 89% (m/m), distribution coefficient of 1.2 and purification fold of 7.0.

  3. Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System.

    Science.gov (United States)

    Tan, Zhi-Jian; Wang, Chao-Yun; Yang, Zi-Zhen; Yi, Yong-Jian; Wang, Hong-Ying; Zhou, Wan-Lai; Li, Fen-Fang

    2015-09-30

    In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid-solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na₂SO₄ at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.

  4. PEG-salt aqueous two-phase systems: an attractive and versatile liquid-liquid extraction technology for the downstream processing of proteins and enzymes.

    Science.gov (United States)

    Glyk, Anna; Scheper, Thomas; Beutel, Sascha

    2015-08-01

    Nowadays, there is an increasing demand to establish new feasible, efficient downstream processing (DSP) techniques in biotechnology and related fields. Although several conventional DSP technologies have been widely employed, they are usually expensive and time-consuming and often provide only low recovery yields. Hence, the DSP is one major bottleneck for the commercialization of biological products. In this context, polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) represent a promising, efficient liquid-liquid extraction technology for the DSP of various biomolecules, such as proteins and enzymes. Furthermore, ATPS can overcome the limitations of traditional DSP techniques and have gained importance for applications in several fields of biotechnology due to versatile advantages over conventional DSP methods, such as biocompatibility, technical simplicity, and easy scale-up potential. In the present review, various practical applications of PEG-salt ATPS are presented to highlight their feasibility to operate as an attractive and versatile liquid-liquid extraction technology for the DSP of proteins and enzymes, thus facilitating the approach of new researchers to this technique. Thereby, single- and multi-stage extraction, several process integration methods, as well as large-scale extraction and purification of proteins regarding technical aspects, scale-up, recycling of process chemicals, and economic aspects are discussed.

  5. Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.

    Science.gov (United States)

    Spir, Lívia Genovez; Ataide, Janaína Artem; De Lencastre Novaes, Letícia Celia; Moriel, Patrícia; Mazzola, Priscila Gava; De Borba Gurpilhares, Daniela; Silveira, Edgar; Pessoa, Adalberto; Tambourgi, Elias Basile

    2015-01-01

    Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential.

  6. An Aqueous Two-Phase System for the Concentration and Extraction of Proteins from the Interface for Detection Using the Lateral-Flow Immunoassay.

    Directory of Open Access Journals (Sweden)

    Ricky Y T Chiu

    Full Text Available The paper-based immunoassay for point-of-care diagnostics is widely used due to its low cost and portability over traditional lab-based assays. Lateral-flow immunoassay (LFA is the most well-established paper-based assay since it is rapid and easy to use. However, the disadvantage of LFA is its lack of sensitivity in some cases where a large sample volume is required, limiting its use as a diagnostic tool. To improve the sensitivity of LFA, we previously reported on the concentration of analytes into one of the two bulk phases of an aqueous two-phase system (ATPS prior to detection. In this study, we preserved the advantages of LFA while significantly improving upon our previous proof-of-concept studies by employing a novel approach of concentrating gold nanoparticles, a common LFA colorimetric indicator. By conjugating specific antibodies and polymers to the surfaces of the particles, these gold nanoprobes (GNPs were able to capture target proteins in the sample and subsequently be concentrated within 10 min at the interface of an ATPS solution comprised of polyethylene glycol, potassium phosphate, and phosphate-buffered saline. These GNPs were then extracted and applied directly to LFA. By combining this prior ATPS interface extraction with LFA, the detection limit of LFA for a model protein was improved by 100-fold from 1 ng/μL to 0.01 ng/μL. Additionally, we examined the behavior of the ATPS system in fetal bovine serum and synthetic urine to more closely approach real-world applications. Despite using more complex matrices, ATPS interface extraction still improved the detection limit by 100-fold within 15 to 25 min, demonstrating the system's potential to be applied to patient samples.

  7. Optimization of Serine Protease Purification from Mango (Mangifera indica cv. Chokanan Peel in Polyethylene Glycol/Dextran Aqueous Two Phase System

    Directory of Open Access Journals (Sweden)

    Abdul Manap Mohd Yazid

    2012-03-01

    Full Text Available Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG/dextran-based aqueous two-phase system (ATPS to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000–12,000 g·mol−1, tie line length (−3.42–35.27%, NaCl (−2.5–11.5% and pH (4.5–10.5 on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2 purification factor (14.37 and yield (97.3% of serine protease were obtained in the presence of 8000 g·mol−1 of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high purification factor and yield with easy scale-up and fast processing.

  8. Two-phase flow in refrigeration systems

    CERN Document Server

    Gu, Junjie; Gan, Zhongxue

    2013-01-01

    Two-Phase Flow in Refrigeration Systems presents recent developments from the authors' extensive research programs on two-phase flow in refrigeration systems. This book covers advanced mass and heat transfer and vapor compression refrigeration systems and shows how the performance of an automotive air-conditioning system is affected through results obtained experimentally and theoretically, specifically with consideration of two-phase flow and oil concentration. The book is ideal for university postgraduate students as a textbook, researchers and professors as an academic reference book, and b

  9. Synthesis of thermo-responsive polymers recycling aqueous two-phase systems and phase formation mechanism with partition of ε-polylysine.

    Science.gov (United States)

    Xu, Chengning; Dong, Wenying; Wan, Junfen; Cao, Xuejun

    2016-11-11

    Aqueous two-phase systems (ATPS) have the potential application in bioseparation and biocatalysis engineering. In this paper, a recyclable ATPS was developed by two thermo-responsive copolymers, PVBAm and PN. Copolymer PVBAm was copolymerized using N-vinylcaprolactam, Butyl methacrylate and Acrylamide as monomers, and PN was synthesized by N-isopropylacrylamide. The lower critical solution temperature (LCST) of PVBAm and PN were 45.0°C and 33.5°C, respectively. The recoveries of both polymers could achieve over 95.0%. The phase behavior and formation mechanism of PVBAm/PN ATPS was studied. Low-field nuclear magnetic resonance (LF-NMR) was applied in the phase-forming mechanism study in ATPS. In addition, combining the analysis results of surface tension, transmission electron microscopy and dynamic light scattering, the phase-forming of the PVBAm/PN ATPS was proved. The application was performed by partition of ε-polylysine in the 2% PVBAm/2% PN (w/w) ATPS. The results demonstrated that ε-polylysine was extracted into the PN-rich phase, the maximal partition coefficient (1/K) and extraction recovery of pure ε-polylysine were 6.87 and 96.36%, respectively, and 7.41 partition coefficient and 97.85% extraction recovery for ε-polylysine fermentation broth were obtained in the presence of 50mM (NH4)2SO4 at room temperature. And this method can effectively remove the most impurities from fermentation broth when (NH4)2SO4 exists in the ATPS. It is believed that the thermo-responsive recycling ATPS has a good application prospect in the field of bio-separation.

  10. Aqueous Nanofluid as a Two-Phase Coolant for PWR

    Directory of Open Access Journals (Sweden)

    Pavel N. Alekseev

    2012-01-01

    Full Text Available Density fluctuations in liquid water consist of two topological kinds of instant molecular clusters. The dense ones have helical hydrogen bonds and the nondense ones are tetrahedral clusters with ice-like hydrogen bonds of water molecules. Helical ordering of protons in the dense water clusters can participate in coherent vibrations. The ramified interface of such incompatible structural elements induces clustering impurities in any aqueous solution. These additives can enhance a heat transfer of water as a two-phase coolant for PWR due to natural forming of nanoparticles with a thermal conductivity higher than water. The aqueous nanofluid as a new condensed matter has a great potential for cooling applications. It is a mixture of liquid water and dispersed phase of extremely fine quasi-solid particles usually less than 50 nm in size with the high thermal conductivity. An alternative approach is the formation of gaseous (oxygen or hydrogen nanoparticles in density fluctuations of water. It is possible to obtain stable nanobubbles that can considerably exceed the molecular solubility of oxygen (hydrogen in water. Such a nanofluid can convert the liquid water in the nonstoichiometric state and change its reduction-oxidation (RedOx potential similarly to adding oxidants (or antioxidants for applying 2D water chemistry to aqueous coolant.

  11. A method for dye extraction using an aqueous two-phase system: Effect of co-occurrence of contaminants in textile industry wastewater.

    Science.gov (United States)

    Borges, Gabriella Alexandre; Silva, Luciana Pereira; Penido, Jussara Alves; de Lemos, Leandro Rodrigues; Mageste, Aparecida Barbosa; Rodrigues, Guilherme Dias

    2016-12-01

    This paper reports a green and efficient procedure for extraction of the dyes Malachite Green (MG), Methylene Blue (MB), and Reactive Red 195 (RR) using an aqueous two-phase system (ATPS). An ATPS consists mainly of water, together with polymer and salt, and does not employ any organic solvent. The extraction efficiency was evaluated by means of the partition coefficients (K) and residual percentages (%R) of the dyes, under different experimental conditions, varying the tie-line length (TLL) of the system, the pH, the type of ATPS-forming electrolyte, and the type of ATPS-forming polymer. For MG, the best removal (K = 4.10 × 10(4), %R = 0.0069%) was obtained with the ATPS: PEO 1500 + Na2C4H4O6 (TLL = 50.21% (w/w), pH = 6.00). For MB, the maximum extraction (K = 559.9, %R = 0.258%) was achieved with the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 1.00). Finally for RR, the method that presented the best results (K = 3.75 × 10(4), %R = 0.237%) was the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 6.00). The method was applied to the recovery of these dyes from a textile effluent sample, resulting in values of K of 1.17 × 10(4), 724.1, and 3.98 × 10(4) for MG, MB, and RR, respectively, while the corresponding %R values were 0.0038, 0.154, and 0.023%, respectively. In addition, the ATPS methodology provided a high degree of color removal (96.5-97.95%) from the textile effluent.

  12. Tunable aqueous polymer-phase impregnated resins-technology-a novel approach to aqueous two-phase extraction.

    Science.gov (United States)

    van Winssen, F A; Merz, J; Schembecker, G

    2014-02-14

    Aqueous Two-Phase Extraction (ATPE) represents a promising unit operation for downstream processing of biotechnological products. The technique provides several advantages such as a biocompatible environment for the extraction of sensitive and biologically active compounds. However, the tendency of some aqueous two-phase systems to form intensive and stable emulsions can lead to long phase separation times causing an increased footprint for the required mixer-settler devices or the need for additional equipment such as centrifuges. In this work, a novel approach to improve ATPE for downstream processing applications called 'Tunable Aqueous Polymer-Phase Impregnated Resins' (TAPPIR(®))-Technology is presented. The technology is based on the immobilization of one aqueous phase inside the pores of a solid support. The second aqueous phase forms the bulk liquid around the impregnated solids. Due to the immobilization of one phase, phase emulsification and phase separation of ATPE are realized in a single step. In this study, a biodegradable and sustainable aqueous two-phase system consisting of aqueous polyethylene glycol/sodiumcitrate solutions was chosen. The impregnation of different macroporous glass and ceramic solids was investigated and could be proven to be stable. Additionally, the separation of the dye Patent blue V was successfully performed with the TAPPIR(®)-Technology. Thus, the "proof of principle" of this technology is presented.

  13. Separation, concentration and determination of chloramphenicol in environment and food using an ionic liquid/salt aqueous two-phase flotation system coupled with high-performance liquid chromatography.

    Science.gov (United States)

    Han, Juan; Wang, Yun; Yu, Cuilan; Li, Chunxiang; Yan, Yongsheng; Liu, Yan; Wang, Liang

    2011-01-31

    Ionic liquid-salt aqueous two-phase flotation (ILATPF) is a novel, green, non-toxic and sensitive samples pretreatment technique. ILATPF coupled with high-performance liquid chromatography (HPLC) was developed for the analysis of chloramphenicol, which combines ionic liquid aqueous two-phase system (ILATPS) based on imidazolium ionic liquid (1-butyl-3-methylimidazolium chloride, [C(4)mim]Cl) and inorganic salt (K(2)HPO(4)) with solvent sublation. In ILATPF systems, phase behaviors of the ILATPF were studied for different types of ionic liquids and salts. The sublation efficiency of chloramphenicol in [C(4)mim]Cl-K(2)HPO(4) ILATPF was influenced by the types of salts, concentration of K(2)HPO(4) in aqueous solution, solution pH, nitrogen flow rate, sublation time and the amount of [C(4)mim]Cl. Under the optimum conditions, the average sublation efficiency is up to 98.5%. The mechanism of ILATPF contains two principal processes. One is the mechanism of IL-salt ILATPS formation, the other is solvent sublation. This method was practical when applied to the analysis of chloramphenicol in lake water, feed water, milk, and honey samples with the linear range of 0.5-500 ng mL(-1). The method yielded limit of detection (LOD) of 0.1 ng mL(-1) and limit of quantification (LOQ) of 0.3 ng mL(-1). The recovery of CAP was 97.1-101.9% from aqueous samples of environmental and food samples by the proposed method. Compared with liquid-liquid extraction, solvent sublation and ionic liquid aqueous two-phase extraction, ILATPF can not only separate and concentrate chloramphenicol with high sublation efficiency, but also efficiently reduce the wastage of IL. This novel technique is much simpler and more environmentally friendly and is suggested to have important applications for the concentration and separation of other small biomolecules.

  14. EFFECT OF ALCOHOL ON AQUEOUS TWO-PHASE SYSTEM PROPERTIES OF SDS/CTAB/H2O/Na2SO4 SYSTEM%醇对正负离子表面活性剂双水相性质的影响

    Institute of Scientific and Technical Information of China (English)

    滕弘霓; 王利

    2004-01-01

    The effect of alcohol on the aqueous two-phase system properties of SDS/CTAB/H2O/Na2SO4 system was studied by the pseudo ternary phase diagram. The electrostatic forces (both attraction and repulsion) between cationic surfactant and anionic surfactant decreased because of the interaction of alcohol hydroxyl group and surfactant polar group. As a result, the phase area width of aqueous two-phase system increased. Long chain alcohol (more than five carbonatoms), which was in high concentration in the SDS/CTAB/H2O/Na2SO4) system, caused the anionic aqueous two-phase system area to vanish and the cationic aqueous two-phase system area to become wider. The CTAB rich phase turned from top phase to bottom phase.

  15. Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol

    Directory of Open Access Journals (Sweden)

    Leonie Asfora Sarubbo

    2004-09-01

    Full Text Available The partitioning of two proteins, bovine serum albumin (BSA and trypsin was studied in an aqueous poly(ethylene glycol(PEG- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.A partição de duas proteínas, albumina de soro bovino (BSA e tripsina foi estudada no sistema bifásico aquoso Polietileno glicol(PEG - Goma do cajueiro. O diagrama de fases foi estabelecido para a Goma do Cajueiro e para PEG de peso molecular 1500 em duas diferentes temperaturas. A influência de vários parâmetros na partição destas proteínas, incluindo concentração dos polímeros, pH, adição de sal e temperatura foi investigada. Os resultados desta pesquisa demonstraram a importância das características da proteína na partição em sistemas bifásicos aquosos.

  16. 黄芩甙在EOPO/盐双水相系统中的分配系数测定及关联%Measurement and Correlation of Partition Coefficients of Baicalin in EOPO/Salt Aqueous Two-Phase Systems

    Institute of Scientific and Technical Information of China (English)

    李伟; 朱自强

    2002-01-01

    The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systemsat 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The partition coefficients of baicalin varied from 10 to 120.The effect of various factors, including tie-line length, salt composition, molecular weight of EOPO, and solution pH, on the partition behaviorwas investigated in EOPO/salt systems. Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsumodel. Good agreement with experimental data is obtained. The average relative deviations are less than 5.0%.

  17. Study on aqueous two-phase extraction of L-phenylalanine by polyethylene glycol/salt system%聚乙二醇/盐双水相萃取L-苯丙氨酸的研究

    Institute of Scientific and Technical Information of China (English)

    孙晨; 刘文举; 刘宁宁

    2012-01-01

    研究了聚乙二醇/盐双水相体系的成相行为及L-苯丙氨酸在双水相中的分配规律,其中包括聚乙二醇的分子量、聚乙二醇质量分数、盐的种类及加入量、L-苯丙氨酸初始浓度和pH对萃取分离的影响。当聚乙二醇1000的质量分数为27%,磷酸氢二钾的质量浓度为O.15g/mL,L-苯丙氨酸的质量浓度为10g/L,体系的pH为8.5时,L-苯丙氨酸的萃取率最高为99.5%,分配系数最大为186.5。%An aqueous two- phase system of polyethylene glycol (PEG)/salt with the behavior of forming aqueous two-phase and the distribution rules of L-phenylalanine in the aqueous two-phase system were studied.Effects of the molecular weight of polyethylene glycol ,the mass fraction of polyethylene glycol ,the types and concentrations of salts,the initial concentration of L-phenylalanine and pH value were investigated.When the mass fraction of PEG1000 was 27% ,the mass concentration of K2HPO4was 0.]5g/mL,the initial concentration of L-phenylalanine was 10g/L and pH value was about 8.5,the highest extraction yield of L-phenylalanine could reach 99.5 %, partition coefficient of L-phenylalanine could reach 186.5.

  18. Pectinases partitioning in aqueous two-phase systems: an integration of the systems poly(ethylene glycol/crude dextran and poly(ethylene glycol/ammonium sulphate

    Directory of Open Access Journals (Sweden)

    STANA N. PEJIN

    2004-04-01

    Full Text Available The partitioning of pectinases in the poly(ethylene glucol4000/ammonium sulpohate system was studied and also its application for enzymes extraction from the top phase of the poly(ethylene glucol4000/crude dextran system. Almost complete one-sided partition of endo-pectinase and exo-pectinase to the bottom phase of the polymer/salt system was achieved at a tie-line length of 37.16 %. The concentration factors were 1.73 and 3.25, respectively. The highest total endo- and exo-pectinase yields (72.41 % and 69.46 %, respectively were obtained by integration of the polymer/polymer system at a tie-line of 8.61 % and a high phase volume ratio and the polymer/salt system at a tie-line of 30.23 % and a low phase volume ratio. Integration of the partitioning at a high tie-line length in the polymer/polimer and a low tie-line length in the polymer/salt system resulted in a total concentration factor of 1.5 and a purification of 1.66 fold for exo-pectinase. The addition of phosphate to this integrated system improved the total concentration factor and purification fold of the activity to 1.73 and 2.14, respectively.

  19. Features of the milk whey protein partitioning in polyethyleneglycol-sodium citrate aqueous two-phase systems with the goal of isolating human alpha-1 antitrypsin expressed in bovine milk.

    Science.gov (United States)

    Boaglio, Andrea; Bassani, Georgina; Picó, Guillermo; Nerli, Bibiana

    2006-06-06

    Partitioning behaviour of the bovine whey proteins (bovine serum albumin, alpha-lactoalbumin and beta-lactoglobulin) and human alpha-1 antitrypsin in aqueous two-phase systems prepared with polyethyleneglycol (molecular masses: 1000, 1450 and 3350)-sodium citrate was analysed at pH 5.2, 6.2 and 8.2. Alpha lactoalbumin concentrated in the polyethyleneglycol rich-phase, while beta-lactoglobulin, bovine serum albumin and alpha-1 antitrypsin showed affinity for the citrate rich-phase. In aqueous two-phase systems of high medium pH and high polyethyleneglycol molecular mass the protein partitioning equilibrium is displaced to the citrate rich-phase. The polyethyleneglycol 1450-pH 5.2 system with a top/bottom phase-volume ratio of 3 showed to have the best capability of recovering the alpha-1 antitrypsin from a mixture prepared with natural milk whey and human alpha-1 antitrypsin. The recovery of this protein in the bottom phase was of 90% and the purity of the obtained product was of 98%. The method appears to be suitable as a starting point to isolate other human proteins expressed in transgenic bovine milk.

  20. PEG-modified Penicillin Acylase and Its Partitioning in Aqueous Two-phase Bioconversion System%PEG修饰青霉素酰化酶及其在两水相生物转化体系中的分配

    Institute of Scientific and Technical Information of China (English)

    何汉平; 刘叶青; 曹学君; 张秀萍

    2001-01-01

    Polyethyleneglycol (PEG) activated by tosyl chloride was used to modify penicillin acylase (PAC). The stability of the PEG-PAC was much better than the free PAC. The activity of the modified enzyme was 75% of free enzyme. The partition of PEG-PAC in aqueous two-phase system was investigated. The modified penicillin acylase was mainly present in the PEG phase in a PEG-dextran aqueous two-phase system, and the partition coefficient was 25.%采用对甲苯磺酰氯法,用聚乙二醇对青霉素酰化酶进行化学修饰,修饰酶的比活为未修饰酶的75%,稳定性比游离酶有了很大提高。酶在带修饰酶的聚乙二醇与葡聚糖组成的两水相体系中的分配系数为25。

  1. Extraction of doxycycline hydrochloride using anhydrous alcohol and acetone binary aqueous two phase system%醇和小分子有机物二元双水相体系萃取盐酸多西环素

    Institute of Scientific and Technical Information of China (English)

    关卫省; 邓江华; 韩娟

    2012-01-01

    基于小分子醇双水相体系和小分子有机物双水相体系,建立了乙醇与丙酮和( NH4) 2SO4形成的二元双水相体系萃取盐酸多西环素的新方法.研究了(NH4)2SO4浓度、pH值、温度、乙醇与丙酮用量和盐酸多西环素浓度对盐酸多西环素分配行为的影响.结果表明,体系的(NH4)2SO4浓度为41%,pH值在4.5 ~5.0,温度25℃,且盐酸多西环素的质量浓度70 mg/L时,萃取率可达93.61%,分配系数可达83.081.%Based on the molecular alcohol aqueous two-phase system and the small molecular organic two-phase system,the new method of using anhydrous alcohol and small molecular organic (acetone) with ( NH4)2SO4 binary aqueous two phase system for extracting doxycycline hydrochloride was set up. The influence factors on partition behaviors of doxycycline hydrochloride were studied, including the concentration of (NH4)2SO4,anhydrous alcohol and acetone consumption,pH value,temperature and doxycycline hydrochloride. The results showed that when (NH4)2SO4 concentration of 41% ,pH value of 4.5~5.0, and the temperature of 25℃ ,mass concentration of doxycycline hydrochloride 70 mg/L,the extraction rate up to 93.61% and 83.081 .respectively.

  2. Extraction of Doxycycline Hydrochloride Using Alcohol and Ionic Liquid Binary Aqueous Two Phase System%醇与离子液体二元双水相体系萃取盐酸多西环素

    Institute of Scientific and Technical Information of China (English)

    关卫省; 黎文娟; 韩娟

    2012-01-01

    Based on the molecular alcohol aqueous two-phase system and the ionic liquid aqueous two-phase system, the new method of using n-propanol and hydrophilic ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim]BF4) with (NH4)2SO4 binary aqueous two phase system for the extraction of doxycycline hydrochloride was developed. The influence factors on partition behaviors of doxycycline hydrochloride were studied, including concentration of (NH4)2SO4, n-propanol con-sumption, pH value, concentration of ionic liquid and doxycycline hydrochloride. The results showed that when using pH value of 4. 0 - 5. 0, (NH4) 2SO4 concentration of 34% and doxycycline hydrochloride concentration between 25 -95 mg/L, the extraction rate and distribution coefficient of doxycycline hydrochloride will be up to 90. 26% -95. 71% and 62. 452 - 149. 401, respectively.%基于小分子醇双水相体系和离子液体双水相体系,建立了正丙醇与亲水性离子液体1-丁基-3-甲基咪唑四氟硼酸[ Bmim]BF4和(NH4 )2SO4形成的二元双水相体系萃取盐酸多西环素的新方法.考察了(NH4)2SO4含量、正丙醇用量、pH值、离子液体含量以及盐酸多西环素含量对盐酸多西环素分配行为的影响.结果表明:当醇和离子液体二元双水相体系的pH值在4.0~5.0范围内,(NH4)2SO4含量为34%,且盐酸多西环素的质量浓度在25 ~ 95 mg/L之间时,该体系对盐酸多西环素的萃取率可达90.26% ~95.71%,分配系数可达62.452 ~ 149.401.

  3. Study on treatment of phenolic compounds in cokery wastewater by propyl-alcohol ammonium sulfate aqueous two-phase system%醇-盐双水相技术去除废水中的酚类物质

    Institute of Scientific and Technical Information of China (English)

    曹文; 高云涛; 王红斌; 李孔明; 马存娇

    2009-01-01

    A novel method for treatment of phenolic compounds in cokery wastewater by propyl-alcohol ammonium sulfate aqueous two-phase system was investigted.The effects of the amount of ammonium sulfate used,the amount of propyl-alcohol used,pH value on extractield was studied.Extraction was optimized using a L9(34)orthogonal test.The results indicated optimum extraction conditions were the amount of ammonium sulfate used of 18.0 g,the amount of propyl-alcohol used of 16 mL,and pH value of 3.0.A extraction yield of 99.37% was achived under optimum extraction conditions and via secondary extraction.

  4. Multistage aqueous two-phase extraction of a monoclonal antibody from cell supernatant.

    Science.gov (United States)

    Muendges, Jan; Zalesko, Alexej; Górak, Andrzej; Zeiner, Tim

    2015-01-01

    This article presents results of continuous multistage aqueous two-phase extraction of an immunoglobulin G1 from cell supernatant in a mixer-settler unit. An aqueous two-phase system consisting of polyethylene glycol 2000, phosphate salt, and water was applied without and with sodium chloride (NaCl). Influences of different parameters such as throughput, phase ratio, and stage number on the extraction performance were analyzed. For systems without NaCl, the extraction was carried out as a washing step. An increase of stage number from one to five stages enabled to increase the immunoglobulin G1 purity from 11.8 to 32.6% at a yield of nearly 90%. Furthermore, a reduction of product phase volume due to a higher phase ratio led to an increase of purity from 20.8 to 29.6% in a three-stage countercurrent extraction. For experiments with NaCl moderate partitioning conditions were adjusted by adding 8 wt% NaCl. In that case, the extraction was carried out as a stripping step.

  5. 异丙醇/盐双水相分离制备高色价栀子黄%Separation of Gardenia Yellow by Isopropanol Alcohol/Salt Aqueous Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    郭晶莹; 张红萍

    2016-01-01

    Abstrat:Gardenia yellow was separated by employing isopropanol-salt aqueous two-phase system. The factors such as kinds of salt, concentration of the salt, pH and temperture on the gardenia yellow separation efficiency were investigated, and the gardenia yellow was analyzed by spectrophotometric method and HPLC. The results showed that the aqueous two-phase system which was formed by isopropanol alcohol(2mL) and sodium citrate (1.6g)was effective for the separation of gardenia yellow from gardenia fruit in 35℃and pH=8.7,and the color value and the OD of the gardenia yellow were 542 and 0.382,respectively.%采用异丙醇/盐组成的双水相体系分离栀子黄。综合考察了盐的种类、浓度、pH值和温度等因素对栀子黄分离效果的影响,并且采用紫外-可见分光光度法和HPLC对异丙醇/盐双水相体系分离得到的栀子黄进行评价。结果表明,在35℃条件下由1.6g的柠檬酸三钠和2mL的异丙醇组成双水相体系,pH等于8.7,分离得到栀子黄和OD值分别为0.382、542。

  6. Continuous aqueous two-phase extraction of human antibodies using a packed column.

    Science.gov (United States)

    Rosa, P A J; Azevedo, A M; Sommerfeld, S; Bäcker, W; Aires-Barros, M R

    2012-01-01

    The performance of a pilot scale packed differential contactor was evaluated for the continuous counter-current aqueous two-phase extraction (ATPE) of human immunoglobulin G (IgG) from a Chinese hamster ovary (CHO) cells supernatant (CS) enriched with pure protein. Preliminary studies have been firstly performed in order to select the dispersed phase (phosphate-rich or polyethylene glycol 3350 Da (PEG)-rich phase) and the column packing material. The PEG-rich phase has been selected as the dispersed phase and the stainless steel as the preferred material for the column packing bed since it was not wetted preferentially by the selected dispersed phase. Hydrodynamic studies have been also performed, and the experimental results were successfully adjusted to the Richardson-Zaki and Mísek equations, typically used for the conventional organic-aqueous two-phase systems. An experimental set-up combining the packed column with a pump mixer-settler stage showed to have the best performance and to be advantageous when compared to the IgG batch extraction. An IgG recovery yield of 85% could be obtained with about 50% of total contaminants and more than 85% of contaminant proteins removal. Mass transfer studies have revealed that the mass transfer was controlled by the PEG-rich phase. A higher efficiency could be obtained when using an extra pump mixer-settler stage and higher flow rates.

  7. Gibbs free energy of transfer of a methylene group on {UCON + (sodium or potassium) phosphate salts} aqueous two-phase systems: Hydrophobicity effects

    OpenAIRE

    Silvério, Sara C.; Rodríguez, Oscar; Teixeira, J. A.; Macedo, Eugénia

    2010-01-01

    The Gibbs free energy of transfer of a suitable hydrophobic probe can be regarded as a measure of the relative hydrophobicity of the different phases. The methylene group (CH2) can be considered hydrophobic, and thus be a suitable probe for hydrophobicity. In this work, the partition coefficients of a series of five dinitrophenylated-amino acids were experimentally determined, at 23 °C, in three different tie-lines of the biphasic systems: (UCON + K2HPO4), (UCON + potassium phosph...

  8. Synthesis of octyl-β-D-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system.

    Science.gov (United States)

    Guo, DongHeng; Xu, YanShan; Kang, YaJun; Han, ShuangYan; Zheng, SuiPing

    2016-04-01

    We explored the ability of a Thai rosewood β-glucosidase-displaying P. pastoris whole-cell biocatalyst (Pp-DCBGL) system to synthesize alkyl β-D-glucosides. The primary investigation centered on the synthesis of octyl-β-D-glucopyranoside (octyl-glu, OG). OG could be synthesized through reverse hydrolysis reaction with very low efficiency. Then, OG was synthesized between BG and octanol by a transglycosylation reaction. In a 2-ml reaction system, OG was synthesized with a conversion rate of 51.1% in 3h when 5 mg/ml BG was utilized as the glucosyl donor under optimized conditions. And, even after being reused four times, the Pp-DCBGL was relatively stable. Additionally, a 500-ml-scale reaction system was conducted in a 2-L stirred reactor with a conversion rate of 47.5% in 1.5 h. Moreover, the conversion rate did not decrease after the whole-cell catalyst was reused two times. In conclusion, Pp-DCBGL has high reaction efficiency and operational stability, which is a powerful biocatalyst available for industrial synthesis.

  9. Study on Extraction Process Conditions of Rice Bran Polysaccharide by Aqueous Two-phase System%双水相萃取米糠多糖工艺条件的探究

    Institute of Scientific and Technical Information of China (English)

    秦微微; 金婷; 宋学东; 丁振铎; 张衡

    2014-01-01

    旨在探究聚乙二醇/硫酸铵双水相体系萃取米糠中多糖成分的最佳条件。以双水相系统的相比、分配系数、收率等为参数,探究了PEG 的相对分子质量、PEG 的质量分数以及硫酸铵质量分数对米糠多糖在两相系统中的分配行为的影响。结果表明:萃取最佳条件为PEG分子量为6000,硫酸铵质量分数为14.8%,PEG 6000质量分数为14.3%,多糖得率为0.826388。%The aqueous two-phase system of polyethylene glycol (PEG)/ammonium sulfate for extrac-tion of polysaccharide in rice bran is studied.Use the parameters such as the volume ratio of two pha-ses,the distribution coefficient and the yield to explore the effects of the molecular weight of PEG, the mass fraction of ammonium sulfate and PEG on distribution behavior of polysaccharide of rice bran in two-phase system.It is demonstrated that the optimum conditions of extraction are as follows:the molecular weight of PEG is 6000,the mass fraction of ammonium sulfate is 14.8%,the mass fraction of PEG 6000 is 14.3%,and the yield of polysaccharide is 0.826388.

  10. Extraction of Solanesol and Nicotine from Inferior Tobacco Leaves by Aqueous Two-phase System%双水相同时提取次烟叶中茄尼醇和烟碱的研究

    Institute of Scientific and Technical Information of China (English)

    刘宝亮; 曹桂萍; 张金涛

    2012-01-01

    The feasibility and technical conditions of the extraction of solanesol and nicotine from inferior tobacco leaves by a new aqueous two-phase system were investigated. An Acetone-DHP (Dipotassium Hydrogen Phosphate) aqueous two-phase system was applied. Factors including the different volume ratio of acetone to water, the addition amount of phosphate,the material liquid ratio,extraction temperature, extraction time and the pH value on the influences of the yield of two substances were studied. The optimized extraction conditions were described as follows: the volume ratio of acetone to water was 5:5,addition amount of phosphate was 3.0 g,material liquid ratio was 0.025 g/mL (0.5 g/20.0 mL) .extraction temperature was 50℃ .extraction time was 4 h and pH was 10.%研究新型双水相体系同时从次等烟丝中提取茄尼醇和烟碱的可行性和工艺条件.新型双水相体系为丙酮-磷酸氢二钾双水相体系,主要研究了丙酮与水不同体积比、磷酸氢二钾的加入量、料液比、浸提温度、浸提时间、双水相体系pH值对茄尼醇和烟碱得率的影响,确定了最佳提取条件:丙酮与水的体积为5:5,磷酸氢二钾的加入量为3.0g,料液比为0.025 g/mL(0.5 g/20.0 mL),浸提温度为50℃,浸提时间为4h,pH值为10.

  11. An integrated practical implementation of continuous aqueous two-phase systems for the recovery of human IgG: From the microdevice to a multistage bench-scale mixer-settler device.

    Science.gov (United States)

    Espitia-Saloma, Edith; Vâzquez-Villegas, Patricia; Rito-Palomares, Marco; Aguilar, Oscar

    2016-05-01

    Aqueous two-phase systems (ATPS) are a liquid-liquid extraction technology with clear process benefits; however, its lack of industrial embracement is still a challenge to overcome. Antibodies are a potential product to be recovered by ATPS in a commercial context. The objective of this work is to present a more integral approach of the different isolated strategies that have arisen in order to enable a practical, generic implementation of ATPS, using human immunoglobulin G (IgG) as experimental model. A microfluidic device is used for ATPS parameters preselection for product recovery. ATPS were continuously operated in a mixer-settler device in one stage, multistage and multistage with recirculation configuration. Single-stage pure IgG extraction with a polyethylene glycol (PEG) 3350-phophates ATPS within continuous operation allowed a 65% recovery. Further implementation of a multistage platform promoted a higher particle partitioning reaching a 90% recovery. The processing of IgG from a cell supernatant culture harvest in a multistage system with top phase recirculation resulted in 78% IgG recovery in bottom phase. This work conjugates three not widely spread methodologies for ATPS: microfluidics, continuous and multistage operation.

  12. A novel aqueous two phase system composed of a thermo-separating polymer and an organic solvent for purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel.

    Science.gov (United States)

    Amid, Mehrnoush; Manap, Yazid; Zohdi, Nor Khanani

    2014-05-22

    The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel for the first time was investigated using a novel aqueous two-phase system (ATPS) consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO) copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR), pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w) EOPO 2500 and 15% (w/w) 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w) at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  13. A Novel Aqueous Two Phase System Composed of a Thermo-Separating Polymer and an Organic Solvent for Purification of Thermo-Acidic Amylase Enzyme from Red Pitaya (Hylocereus polyrhizus Peel

    Directory of Open Access Journals (Sweden)

    Mehrnoush Amid

    2014-05-01

    Full Text Available The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus peel for the first time was investigated using a novel aqueous two-phase system (ATPS consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO copolymer and type and concentration of organic solvent on the partitioning behavior of amylase was investigated. In addition, the effects of phase components, volume ratio (VR, pH and crude load of purification factor and yield of amylase were evaluated to achieve the optimum partition conditions of the enzyme. In the novel ATPS method, the enzyme was satisfactorily partitioned into the polymer-rich top phase in the system composed of 30% (w/w EOPO 2500 and 15% (w/w 2-propanol, at a volume ratio of 1.94 and with a crude load scale of 25% (w/w at pH 5.0. Recovery and recycling of components was also measured in each successive step of the ATPS process. The enzyme was successfully recovered by the method with a high purification factor of 14.3 and yield of 96.6% and copolymer was also recovered and recycled at a rate above 97%, making the method was more economical than the traditional ATPS method.

  14. Multiparametric Analysis of Oncology Drug Screening with Aqueous Two-Phase Tumor Spheroids.

    Science.gov (United States)

    Shahi Thakuri, Pradip; Ham, Stephanie L; Luker, Gary D; Tavana, Hossein

    2016-11-07

    Spheroids present a biologically relevant three-dimensional model of avascular tumors and a unique tool for discovery of anticancer drugs. Despite being used in research laboratories for several decades, spheroids are not routinely used in the mainstream drug discovery pipeline primarily due to the difficulty of mass-producing uniformly sized spheroids and intense labor involved in handling, drug treatment, and analyzing spheroids. We overcome this barrier using a polymeric aqueous two-phase microtechnology to robotically microprint spheroids of well-defined size in standard 384-microwell plates. We use different cancer cells and show that resulting spheroids grow over time and display characteristic features of solid tumors. We demonstrate the feasibility of robotic, high-throughput screening of 25 standard chemotherapeutics and molecular inhibitors against tumor spheroids of three different cancer cell lines. This screening uses over 7000 spheroids to elicit high quality dose-dependent drug responses from spheroids. To quantitatively compare performance of different drugs, we employ a multiparametric scoring system using half-maximum inhibitory concentration (IC50), maximum inhibition (Emax), and area under the dose-response curve (AUC) to take into account both potency and efficacy parameters. This approach allows us to identify several compounds that effectively inhibit growth of spheroids and compromise cellular viability, and distinguish them from moderately effective and ineffective drugs. Using protein expression analysis, we demonstrate that spheroids generated with the aqueous two-phase microtechnology reliably resolve molecular targets of drug compounds. Incorporating this low-cost and convenient-to-use tumor spheroid technology in preclinical drug discovery will make compound screening with realistic tumor models a routine laboratory technique prior to expensive and tedious animal tests to dramatically improve testing throughput and efficiency and

  15. Aqueous two-phase partition applied to the isolation of plasma membranes and Golgi apparatus from cultured mammalian cells

    Science.gov (United States)

    Morre, D. M.; Morre, D. J.

    2000-01-01

    Partitioning in dextran-poly(ethylene)glycol (PEG) aqueous-aqueous phase systems represents a mature technology with many applications to separations of cells and to the preparation of membranes from mammalian cells. Most applications to membrane isolation and purification have focused on plasma membranes, plasma membrane domains and separation of right side-out and inside-out plasma membrane vesicles. The method exploits a combination of membrane properties, including charge and hydrophobicity. Purification is based upon differential distributions of the constituents in a sample between the two principal compartments of the two phases (upper and lower) and at the interface. The order of affinity of animal cell membranes for the upper phase is: endoplasmic reticulum Golgi apparatusGolgi apparatus from transformed mammalian cells that combines aqueous two-phase partition and centrifugation. Also described is a periodic NADH oxidase, a new enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions for measurement of activity.

  16. Partition efficiencies of newly fabricated universal high-speed counter-current chromatograph for separation of two different types of sugar derivatives with organic-aqueous two-phase solvent systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Sato, Kazuki; Yoshida, Kazunori; Tokura, Koji; Maruyama, Hiroshi; Yanagidaira, Kazuhiro; Ito, Yoichiro

    2013-12-27

    A new design of universal high-speed counter-current chromatograph (HSCCC) was fabricated in our laboratory. It holds a set of four column holders symmetrically around the rotary frame at a distance of 11.2cm from the central axis. By engaging the stationary gear on the central axis of the centrifuge to the planetary gears on the column holder shaft through a set of idle gears, two pairs of diagonally located column holders simultaneously rotate about their own axes in the opposite directions: one forward (type-J planetary motion) and the other backward (type-I planetary motion) each synchronously with the revolution. Using the eccentric coil assembly, partition efficiencies produced by these two planetary motions were compared on the separation of two different types of sugar derivatives (4-methylumbelliferyl and 5-bromo-4-chloro-3-indoxyl sugar derivatives) using organic-aqueous two-phase solvent systems composed of n-hexane/ethyl acetate/1-butanol/methanol/water and aqueous 0.1M sodium tetraborate, respectively. With lower phase mobile, better peak resolution was obtained by the type-J forward rotation for both samples probably due to higher retention of the stationary phase. With upper phase mobile, however, similar peak resolutions were obtained between these two planetary motions for both sugar derivatives. The overall results indicate that the present universal HSCCC is useful for counter-current chromatographic separation since each planetary motion has its specific applications: e.g., vortex CCC by the type-I planetary motion and HSCCC by the type-J planetary motion both for separation of various natural and synthetic products.

  17. Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system%木瓜蛋白酶在离子液体双水相中的分配行为

    Institute of Scientific and Technical Information of China (English)

    王伟涛; 蒋志国; 张海德; 彭健; 许英豪; 董安华; 杨雪芳; 蒋欣欣

    2015-01-01

    Papain was extracted by ionic liquid aqueous two-phase system. Firstly,the influence of concentration, pH, and temperature of ionic liquid on the activity of papain was investigated. Secondly, the effects of different ionic liquid aqueous two-phase systems, alkyl chain lengths and concentrations of the ionic liquid, dosage of papain, pH, temperature on the partitioning behavior of papain were discussed. [C4mim]Cl and [C4mim]Br systems were better than [C4mim]BF4 system in extraction of papain, and it was disadvantageous to extract papain at a high temperature (60℃or higher). Activity recovery of enzyme reached 95.16%and purification factor reached 1.5 under the optimum conditions:[C4mim]Cl 0.25 g·ml−1, K2HPO4 0.35 g·ml−1, pH 8.0, enzyme addition 2.0 mg·ml−1, 30℃. The result laid the experimental basis for further scale-up research and commercial production.%采用离子液体双水相提取木瓜蛋白酶。首先考察不同浓度、pH、温度的离子液体对木瓜蛋白酶活性的影响,其次考察不同离子液体双水相体系、离子液体侧烷基链长度及浓度、酶添加量、pH、温度对木瓜蛋白酶分配行为的影响。结果表明:[C4mim]Cl 和[C4mim]Br 体系萃取木瓜蛋白酶的效果比[C4mim]BF4体系好;高温(≥60℃)对离子液体双水相体系萃取木瓜蛋白酶不利。离子液体双水相萃取木瓜蛋白酶的最佳工艺条件:0.25 g·ml−1的[C4mim]Cl,0.35 g·ml−1的K2HPO4,pH 8.0,酶添加量2.0 mg·ml−1,30℃。此条件下木瓜蛋白酶的酶活性回收率达到95.16%,纯化因子达到1.5。为今后进一步研究该体系的放大实验或规模化生产奠定了基础。

  18. Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase poly(ethyleneglycol/phosphate systems Purificação parcial de glucose-6-fosfato desidrogenase por sistemas de duas fases aquosas poli (etilenoglicol/fosfato

    Directory of Open Access Journals (Sweden)

    Marcela Zanella Ribeiro

    2007-03-01

    Full Text Available Glucose-6-phosphate dehydrogenase (G6PDH is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS of poly(ethyleneglycol (PEG/phosphate buffer, using as enzyme source a medium prepared through commercial baker's yeast disruption. Firstly, the effects of PEG molar mass on the enzyme partition and of homogenization and rest on the system equilibrium were investigated. Afterwards, several ATPS were prepared using statistical analysis (2² factorial design. The results, including kinetic and thermodynamic parameters for the G6PDH activity, showed partial purification of this enzyme in ATPS composed of 17.5% (w/w PEG400 and 15.0% (w/w phosphate. A high enzymatic recovery value (97.7%, a high partition coefficient (351, and an acceptable purification factor (2.28 times higher than in cell homogenate were attained from the top phase. So, it was possible to attain an effective enzyme pre-purification by separating some contaminants with a simple method such as liquid-liquid extraction in aqueous two-phase systems (ATPS.Glicose-6-fosfato desidrogenase (G6PDH é uma importante enzima usada em estudos bioquímicos e médicos, bem como em diversos métodos analíticos com aplicação comercial e industrial. Neste trabalho foi avaliado a extração da G6PDH em sistemas de duas fases aquosas (ATPS constituídos por poli(etilenoglicol (PEG/tampão fosfato, usando como fonte de enzima um meio preparado por rompimento de leveduras de panificação comercial. Inicialmente foram investigados os efeitos da massa molar do PEG na partição da enzima e da homogeneização e repouso no equilíbrio do sistema. Na sequência, diversos ATPS foram preparados usando análise estatística (planejamento fatorial 2². Os resultados, incluindo parâmetros cinéticos e termodinâmicos para a atividade da G6PDH

  19. Metallic and semiconducting carbon nanotubes separation using an aqueous two-phase separation technique: a review

    Science.gov (United States)

    Tang, Malcolm S. Y.; Ng, Eng-Poh; Juan, Joon Ching; Ooi, Chien Wei; Ling, Tau Chuan; Woon, Kai Lin; Loke Show, Pau

    2016-08-01

    It is known that carbon nanotubes show desirable physical and chemical properties with a wide array of potential applications. Nonetheless, their potential has been hampered by the difficulties in acquiring high purity, chiral-specific tubes. Considerable advancement has been made in terms of the purification of carbon nanotubes, for instance chemical oxidation, physical separation, and myriad combinations of physical and chemical methods. The aqueous two-phase separation technique has recently been demonstrated to be able to sort carbon nanotubes based on their chirality. The technique requires low cost polymers and salt, and is able to sort the tubes based on their diameter as well as metallicity. In this review, we aim to provide a review that could stimulate innovative thought on the progress of a carbon nanotubes sorting method using the aqueous two-phase separation method, and present possible future work and an outlook that could enhance the methodology.

  20. Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction

    OpenAIRE

    2008-01-01

    Separation of Recombinant à -Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction Kristin Coby Ross Abstract Biopharmaceutical manufacturing is a rigorous and expensive process. Due to the medicinal nature of the product, a high purity level is required and several expensive purification steps must be utilized. Cost-effective production and purification is essential for any biopharmaceutical product to be successful and development of the fastest, most economical, ...

  1. Scaling of Two-Phase Systems Across Gravity Levels Project

    Data.gov (United States)

    National Aeronautics and Space Administration — There is a defined need for long term earth based testing for the development and deployment of two-phase flow systems in reduced-gravity, including lunar gravity,...

  2. Application of aqueous two-phase systems for the development of a new method of cobalt(II), iron(III) and nickel(II) extraction: a green chemistry approach.

    Science.gov (United States)

    Patrício, Pamela da Rocha; Mesquita, Maiby Cabral; da Silva, Luis Henrique Mendes; da Silva, Maria C Hespanhol

    2011-10-15

    We have investigated the extraction behavior of the metallic ions Co(II), Fe(III) and Ni(II) as a function of the amount of potassium thiocyanate used as an extracting agent, using the following aqueous two-phase systems (ATPS): PEO + (NH(4))(2)SO(4) + H(2)O, PEO + Li(2)SO(4) + H(2)O, L35 + (NH(4))(2)SO(4) + H(2)O and L35 + (Li)(2)SO(4)+H(2)O. Metal extraction from the salt-rich phase to the polymer-rich phase is affected by the following parameters: amount of added extractant, pH, and the nature of the electrolyte and polymer that forms the ATPS. Maximal extraction percentages were obtained for Co(II) (99.8%), Fe(III) (12.7%) and Ni(II) (3.17%) when the ATPS was composed of PEO1500 + (NH(4))(2)SO(4) + H(2)O containing 1.4 mmol of KSCN at pH 4.0, providing separation factors as high as S(Co, Fe) = 3440 and S(Co, Ni) = 15,300. However, when the same ATPS was used at pH 2.0, the maximal extraction percentages for iron and nickel were 99.5% and 4.34%, respectively, with S(Fe, Ni) equal to 4380. The proposed technique was shown to be efficient in the extraction of Co(II) and Fe(III), with large viability for the selective separation of Co(II) and Fe(III) ions in the presence of Ni(II).

  3. Response Surface Methodology Modelling of an Aqueous Two-Phase System for Purification of Protease from Penicillium candidum (PCA 1/TT031 under Solid State Fermentation and Its Biochemical Characterization

    Directory of Open Access Journals (Sweden)

    Amaal M. Alhelli

    2016-11-01

    Full Text Available Penicillium candidum (PCA 1/TT031 synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG/citrate based on an aqueous two-phase system (ATPS and Response Surface Methodology (RSM to purify protease from Penicillium candidum (PCA 1/TT031. The effects of different PEG molecular weights (1500–10,000 g/mol, PEG concentration (9%–20%, concentrations of NaCl (0%–10% and the citrate buffer (8%–16% on protease were also studied. The best protease purification could be achieved under the conditions of 9.0% (w/w PEG 8000, 5.2% NaCl, and 15.9% sodium citrate concentration, which resulted in a one-sided protease partitioning for the bottom phase with a partition coefficient of 0.2, a 6.8-fold protease purification factor, and a yield of 93%. The response surface models displayed a significant (p ≤ 0.05 response which was fit for the variables that were studied as well as a high coefficient of determination (R2. Similarly, the predicted and observed values displayed no significant (p > 0.05 differences. In addition, our enzyme characterization study revealed that Penicillium candidum (PCA 1/TT031 produced a slight neutral protease with a molecular weight between 100 and 140 kDa. The optimal activity of the purified enzyme occurred at a pH of 6.0 and at a temperature of 50 °C. The stability between different pH and temperature ranges along with the effect of chemical metal ions and inhibitors were also studied. Our results reveal that the purified enzyme could be used in the dairy industry such as in accelerated cheese ripening.

  4. Extraction of Tartrazine from Food Colorants by PEG/Salt Aqueous Two-Phase System%PEG/盐双水相萃取食用色素柠檬黄的研究

    Institute of Scientific and Technical Information of China (English)

    姜彬; 李冬梅; 冯志彪

    2014-01-01

    采用PEG/盐双水相体系萃取食用色素柠檬黄。考察了成相物质对柠檬黄吸光度的影响,在此基础上考察了PEG相对分子量、PEG浓度、盐的种类及浓度、p H 对萃取效果的影响,确定了萃取柠檬黄的最佳条件:18% PEG2000,15%(NH4)2SO4,pH 为6。该体系对柠檬黄色萃取效果不受外加无机盐的影响,但表面活性剂的存在对萃取效果有较大影响。%An aqueous two-phase system (ATPS )is presented with polyethylene glycol (PEG )and salt for the extraction of tartrazine in this paper.The extraction of tartrazine in ATPS is investigated. Concentration of (NH4 )2 SO4 and PEG,pH value and other factors are evaluated to determine their effects on the extraction yield of tartrazine.The results show that the extraction yield strongly de-pends on the concentration of PEG and (NH4 )2 SO4 .A high extraction yield around 98% is achieved with the following parameters:(NH4)2SO4 15%,PEG2000 18%,pH 6.It is found that the extrac-tion yield undepends on the additional salt,but the presence of surfactant influences the extraction yield evidently.

  5. Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins.

    Science.gov (United States)

    Gai, Qingqing; Qu, Feng; Zhang, Tao; Zhang, Yukui

    2011-07-15

    Both of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein separation. Selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution pH and ionic strength. Aqueous two-phase system of PEG/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. The two combination ways, magnetic adsorption followed by ATPE and ATPE followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome C and myloglobin were discussed and compared. The way of magnetic adsorption followed by ATPE was also applied to human serum separation.

  6. Purification and characterization of polyphenol oxidase from waste potato peel by aqueous two-phase extraction.

    Science.gov (United States)

    Niphadkar, Sonali S; Vetal, Mangesh D; Rathod, Virendra K

    2015-01-01

    Potato peel from food industrial waste is a good source of polyphenol oxidase (PPO). This work illustrates the application of an aqueous two-phase system (ATPS) for the extraction and purification of PPO from potato peel. ATPS was composed of polyethylene glycol (PEG) and potassium phosphate buffer. Effect of different process parameters, namely, PEG, potassium phosphate buffer, NaCl concentration, and pH of the system, on partition coefficient, purification factor, and yield of PPO enzyme were evaluated. Response surface methodology (RSM) was utilized as a statistical tool for the optimization of ATPS. Optimized experimental conditions were found to be PEG1500 17.62% (w/w), potassium phosphate buffer 15.11% (w/w), and NaCl 2.08 mM at pH 7. At optimized condition, maximum partition coefficient, purification factor, and yield were found to be 3.7, 4.5, and 77.8%, respectively. After partial purification of PPO from ATPS, further purification was done by gel chromatography where its purity was increased up to 12.6-fold. The purified PPO enzyme was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by Km value 3.3 mM, and Vmax value 3333 U/mL, and enzyme stable ranges for temperature and pH of PPO were determined. These results revealed that ATPS would be an attractive option for obtaining purified PPO from waste potato peel.

  7. Aqueous two-phase partition applied to the isolation of plasma membranes and Golgi apparatus from cultured mammalian cells

    Science.gov (United States)

    Morre, D. M.; Morre, D. J.

    2000-01-01

    Partitioning in dextran-poly(ethylene)glycol (PEG) aqueous-aqueous phase systems represents a mature technology with many applications to separations of cells and to the preparation of membranes from mammalian cells. Most applications to membrane isolation and purification have focused on plasma membranes, plasma membrane domains and separation of right side-out and inside-out plasma membrane vesicles. The method exploits a combination of membrane properties, including charge and hydrophobicity. Purification is based upon differential distributions of the constituents in a sample between the two principal compartments of the two phases (upper and lower) and at the interface. The order of affinity of animal cell membranes for the upper phase is: endoplasmic reticulum two-phase partition with other procedures to obtain a more highly purified preparation. A procedure is described for preparation of Golgi apparatus from transformed mammalian cells that combines aqueous two-phase partition and centrifugation. Also described is a periodic NADH oxidase, a new enzyme marker for right side-out plasma membrane vesicles not requiring detergent disruptions for measurement of activity.

  8. Partitioning of Bovine Serum Albumin in Polyethylene Glycol(PEG)/Phosphate Aqueous Two-Phase System%PEG/磷酸盐双水相系统萃取BSA的研究

    Institute of Scientific and Technical Information of China (English)

    胡松青; 李琳; 李冰; 肖蕾

    2002-01-01

    BSA partitioning was performed in the PEG/phosphate aqueous two-phase system. The effects of the phase compositions concentrations, addition of NaCl and the initial concentration of BSA on the partition coefficient K and the fraction of BSA in the bottom phase Y were investigated. The results showed that BSA was enriched to the bottom phase. The phase compositions of PEG 6000 mass fraction is 10%/PO3-4 mass fraction is 6% and PEG 10000 mass fraction is 6%/PO3-4 mass fraction is 6% respectively were desirable for partitioning. The addition of NaCl showed no influence on BSA partitioning in PEG 6000 mass fraction is 10% /PO3-4 mass fraction is 6% system. The mathematical model for K was derived from the initial mass concentration of BSA and well fitting with the experimental data.%以PEG/磷酸盐双水相系统萃取BSA为对象,研究了双水相系统成相浓度、外加盐NaCl、BSA起始浓度等对BSA在两相间的分配系数和BSA下相萃取率的影响.结果发现,BSA大部分被萃取入双水相体系的下相,成相组成为PEG 6000 10% (质量分数)/ PO3-4 6% (质量分数)和PEG 10000 6% (质量分数)/ PO3-4 6% (质量分数)的双水相系统有利于BSA的萃取分配;外加NaCl对PEG 6000 10%(质量分数)/PO3-4 6%(质量分数)系统萃取BSA的影响不大;得出了上、下相BSA浓度和BSA在两相间分配系数K与系统BSA起始浓度之间的表达式,与实验结果有较好的拟合.

  9. 离子液体双水相萃取山楂黄酮和多糖的研究%Study on the Extraction of Hawthorn Flavonoids and Polysaccharide in Ionic Liquid Aqueous Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    巩育军; 牛盛童; 黄学锋; 王键

    2014-01-01

    Partition behaviors of hawthorn flavonoids and polysaccharide in ionic liquid aqueous two-phase system(ILATPS)were studied by spectrophotometry.Effects of concentration of ionic liquid and ammonium sulfate,dosage of hawthorn and ultrasonic time on the extraction rate of hawthorn flavonoids and polysaccharide were investigated.The optimal extraction conditions were as follows:concentration of ionic liquid [Bmim]BF4 was 0.26~0.30 g·mL-1,concentration of ammonium sulfate was 0.08~0.10 g·mL-1,dosage of hawthorn was 0.14~0.17 g,ultrasonic time was 15~20 min.Under these conditions,extraction rate of hawthorn fla-vonoids was 86.4%~96.0% in the up phase,and extraction rate of polysaccharide was 75.2%~76.0% in the down phase.%采用分光光度法研究了山楂黄酮和多糖在[Bmim]BF4/(NH4)2 SO4双水相体系的分配行为,探讨了离子液体浓度、(NH4)2 SO4浓度、山楂用量和超声时间等因素对山楂黄酮和多糖萃取率的影响。确定最佳萃取条件为:离子液体[Bmim]BF4浓度0.26~0.30 g·mL-1,(NH4)2 SO4浓度0.08~0.10 g·mL-1,山楂用量0.14~0.17 g,超声时间15~20 min,在此优化条件下,双水相上相中黄酮的萃取率为86.4%~96.0%、下相中多糖的萃取率为75.2%~76.0%。

  10. Extraction of spirulina platensis polysaccharide by ethanol/(NH4)2SO4 aqueous two-phase system%乙醇/硫酸铵双水相体系萃取螺旋藻多糖的研究

    Institute of Scientific and Technical Information of China (English)

    刘杨; 冯元琦; 陈美欣; 吴景淳; 黄海根

    2012-01-01

    Extraction of spirulina platensis polysaccharide by ethanol/( NH4 ) 2SO4 aqueous two-phase system(ATPS)was investigated. To remove and recycle the proteins, salting-out precipitation protein was carried out after spirulina platensis cells disruption. Spirulina platensis polysaccharide was extracted by traditional hot water diffusion and the polysaccharide dissolution rate reached 38.44 ± 1. 12mg/g spirulina platensis powder. Spirulina platensis polysaccharide was purified deeply by ethanol/ (NH4)2SO4 ATPS. The yield and enrichment factor of the polysaccharide were 84. 5±1. 45% and 6. 2 respectively,on the condition that the tie line length, volume ratio and pH of ATPS were 42. 9,1. 05 and 7. 0, respectively. The results indicated that spirulina platensis polysaccharide would be purified by a simple, efficient and low cost extraction technology using inexpensive ethanol/ (NH4)2SO4 ATPS.%考察了低分子有机溶剂与无机盐-乙醇/硫酸铵双水相体系萃取螺旋藻多糖的可行性及影响因素.研究结果表明:为除去并有效回收螺旋藻细胞中的蛋白质成分,在藻细胞破碎后进行盐析沉淀蛋白质,再采用传统的热水浸提法,可得到螺旋藻多糖的溶出率为38.44±1.12mg/g干燥粉;通过乙醇/硫酸铵双水相体系的萃取分配,在w(乙醇)=19%,w(硫酸铵)=27.5%(即双水相体系系线长度TLL =42.9),体系相比VR=1.05,pH=7.0时,螺旋藻多糖的收率可达84.5±1.45%,富集因子可达6.2.该研究结果表明廉价的乙醇/硫酸铵双水相萃取螺旋藻多糖将有望开发成为一条简洁、高效、低成本的螺旋藻多糖分离提取工艺.

  11. Design of a coil satellite centrifuge and its performance on counter-current chromatographic separation of 4-methylumbelliferyl sugar derivatives with polar organic-aqueous two-phase solvent systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Tokura, Koji; Kimura, Emiru; Takai, Midori; Harikai, Naoki; Yoshida, Kazunori; Yanagidaira, Kazuhiro; Ito, Yoichiro

    2015-05-01

    A new high-speed counter-current chromatograph, named coil satellite centrifuge (CSC), was designed and fabricated in our laboratory. The CSC apparatus produces the satellite motion such that the coiled column simultaneously rotates around the sun axis (the angular velocity, ω1), the planet axis (ω2) and the satellite axis (the central axis of the column) (ω3). In order to achieve this triplicate rotary motion without twisting of the flow tube, the rotation of each axis was determined by the following formula: ω1=ω2+ω3. This relation enabled to lay out the flow tube without twisting by the simultaneous rotation of three axes. The flow tube was introduced from the bottom side of the apparatus into the sun axis of the first rotary frame reaching the upper side of the planet axis and connected to the column in the satellite axis. The performance of the apparatus was examined on separation of 4-methylumbelliferyl (MU) sugar derivatives as test samples with organic-aqueous two-phase solvent systems composed of ethyl acetate/1-butanol/water (3:2:5, v/v) for lower phase mobile and (1:4:5, v/v) for upper phase mobile. With lower phase mobile, five 4-MU sugar derivatives including β-D-cellobioside (Cel), β-D-glucopyranoside, α-D-mannopyranoside, β-D-fucopyranoside and α-L-fucopyranoside (α-L-Fuc) were separated with the combined rotation around each axis at counterclockwise (CCW) (ω1) - CCW (ω2) - CCW (ω3) by the flow tube distribution. With upper phase mobile, three 4-MU sugar derivatives including α-L-Fuc, β-D-galactopyranoside and Cel were separated with the combined rotation around each axis at clockwise (CW) (ω1) - CW (ω2) - CW (ω3) by the flow tube distribution. A series of experiments on peak resolution and stationary phase retention revealed that better partition efficiencies were obtained at the flow rate of 0.5 mL/min (column 1) and 0.8 mL/min (column 2) for lower phase mobile and 0.2 mL/min (column 1) and 0.4 mL/min (column 2) for upper phase

  12. Extraction and isolation of lithospermic acid B from Salvia miltiorrhiza Bunge using aqueous two-phase extraction followed by high-performance liquid chromatography.

    Science.gov (United States)

    Guo, Yong Xue; Shi, Chang Zhi; Zhang, Lei; Lv, Lin; Zhang, Yue Yong

    2016-09-01

    A rapid and effective method integrating separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge was developed by combining an aqueous two-phase system extraction with preparative chromatography. An aqueous two-phase system of n-butyl alcohol/KH2 PO4 was chosen from seven systems. The influence of parameters including concentration of KH2 PO4 , n-butyl alcohol concentration, pH, and the ratio of an aqueous two-phase system to crude extract were investigated using a single factor design. Response surface methodology was subsequently used to find the optimal compositions of an aqueous two-phase system. Keeping a solvent-to-solid ratio of 10, the final optimized composition of an aqueous two-phase system was 39.1% w/w n-butyl alcohol and 22.6% w/w KH2 PO4 . Under these conditions a recovery yield of 99.8% and a high partition coefficient of 310.4 were obtained. In a pilot-scale experiment using optimized conditions, 18.79 g of lithospermic acid B with a purity of 70.5% and in a yield of 99.8% was separated from 0.5 kg of crude extract. Subsequently, 9.94 g lithospermic acid B with a purity of 99.3% and recovery yield of 70.3% was obtained with a preparative chromatographic process, and the two-step total recovery was 70.1%.

  13. Recovery of endo-polygalacturonase using polyethylene glycol-salt aqueous two-phase extraction with polymer recycling

    OpenAIRE

    Wu, You-Ting; Pereira, Martinha; Venâncio, Armando; Teixeira, J. A.

    2000-01-01

    The partitioning behaviour of endo-polygalacturonase (endo-PG) and total protein from a clarified Kluyveromyces marxianus fermentation broth in polyethylene glycol (PEG)-ammonium sulfate and PEG-potassium phosphate (pH=7) aqueous two-phase systems was experimentally investigated. Both the enzyme and total protein partitioned in the bottom phase for these two kinds of systems. The enzyme partitioning coefficient can be lower than 0.01 in PEG8000-(NH4)2SO4 ATPS with a large phase volume ratio a...

  14. Camomile autofermentation in polyethylene glycol/dextran two-phase system

    Directory of Open Access Journals (Sweden)

    Đaković Sanja D.

    2008-01-01

    Full Text Available The objective of this study was the investigation of the extractive bioconversion of apigenin-7-O-β-glucoside in camomile ligulate flowers into apigenin by autofermentation in polyethylene glycol 6000/dextran 200000 two-phase system. In 22.5% polyethylene glycol/14% dextran aqueous two-phase system the obtained yield of apigenin in the top phase was 96.5%. In the presence of plant material that partiotioned to the interphase, the yield of apigenin in the top phase was 3.5 times higher in comparison to the model system.

  15. Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis.

    Science.gov (United States)

    Oppermann, Sebastian; Stein, Florian; Kragl, Udo

    2011-02-01

    The development of biotechnological processes using novel two-phase systems based on molten salts known as ionic liquids (ILs) got into the focus of interest. Many new approaches for the beneficial application of the interesting solvent have been published over the last years. ILs bring beneficial properties compared to organic solvents like nonflammability and nonvolatility. There are two possible ways to use the ILs: first, the hydrophobic ones as a substitute for organic solvents in pure two-phase systems with water and second, the hydrophilic ones in aqueous two-phase systems (ATPS). To effectively utilise IL-based two-phase systems or IL-based ATPS in biotechnology, extensive experimental work is required to gain the optimal system parameters to ensure selective extraction of the product of interest. This review will focus on the most actual findings dealing with the basic driving forces for the target extraction in IL-based ATPS as well as presenting some selected examples for the beneficial application of ILs as a substitute for organic solvents. Besides the research focusing on IL-based two-phase systems, the "green aspect" of ILs, due to their negligible vapour pressure, is widely discussed. We will present the newest results concerning ecotoxicity of ILs to get an overview of the state of the art concerning ILs and their utilisation in novel two-phase systems in biotechnology.

  16. Solubilization of proteins in aqueous two-phase extraction through combinations of phase-formers and displacement agents.

    Science.gov (United States)

    Kress, Christian; Sadowski, Gabriele; Brandenbusch, Christoph

    2017-03-01

    The aqueous two-phase extraction (ATPE) of therapeutic proteins is a promising separation alternative to cost-intensive chromatography, still being the workhorse of nowadays downstream processing. As shown in many publications, using NaCl as displacement agent in salt-polymer ATPE allows for a selective purification of the target protein immunoglobulin G (IgG) from human serum albumin (HSA, represents the impurity). However a high yield of the target protein is only achievable as long as the protein is stabilized in solution and not precipitated. In this work the combined influence of NaCl and polyethylene glycol (Mw=2000g/mol) on the IgG-IgG interactions was determined using composition gradient multi-angle light scattering (CG-MALS) demonstrating that NaCl induces a solubilization of IgG in polyethylene glycol 2000 solution. Moreover it is shown that the displacement agent NaCl has a significant and beneficial influence on the IgG solubility in polyethyleneglycol2000-citrate aqueous two-phase system (ATPS) which can also be accessed by these advanced B22 measurements. By simultaneous consideration of IgG solubility data with results of the ATPS phase behavior (especially volume fraction of the respective phases) allows for the selection of process tailored ATPS including identification of the maximum protein feed concentration. Through this approach an ATPS optimization is accessible providing high yields and selectivity of the target protein (IgG).

  17. Two-phase flow instability in a parallel multichannel system

    Institute of Scientific and Technical Information of China (English)

    HOU Suxia

    2009-01-01

    The two-phase flow instabilities observed in through parallel multichannel can be classified into three types, of which only one is intrinsic to parallel multichannel systems. The intrinsic instabilities observed in parallel multichannel system have been studied experimentally. The stable boundary of the flow in such a parallel-channel system are sought, and the nature of inlet flow oscillation in the unstable region has been examined experimentally under various conditions of inlet velocity, heat flux, liquid temperature, cross section of channel and entrance throttling. The results show that parallel multichannel system possess a characteristic oscillation that is quite independent of the magnitude and duration of the initial disturbance, and the stable boundary is influenced by the characteristic frequency of the system as well as by the exit quality when this is low, and upon raising the exit quality and reducing the characteristic frequency, the system increases its instability, and entrance throttling effectively contributes to stabilization of the system.

  18. AQUEOUS TWO-PHASE GAS FLOATATION SPECTROPHOTOMETRIC DETERMINATION OF TRACE TETRACYCLINE IN ENVIRONMENTAL WATER SAMPLE

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A green method for separating and enriching trace tetracycline (TC) in environment water by Aqueous Two-phase Gas Floatation Spectrophotometry has been proposed, the principium was discussed. In this paper, the hydrophobic complex composed of Mg(II) and TC was floated into organic phase under the optimal conditions: pH=10, the floatation equipment is home-made, n-propyl alcohol as the organic solvent, sodium chloride as the separating phase reagent. The data were obtained by spectrophotometry after floatation; The linear regression equation is A=2.33×105C(mol/L)+0.2179, linear range is from 3.77×10-7mol/L to 6.32×10-5mol/L, respectively, with the correlation coefficient (r) better than 0.9997, relative recoveries is 99.7% to 100.3%, limit of detection was 4.29×10-8mol/L, The method can be applied to analyse the trace TC in water sample, the result is better.

  19. AQUEOUS TWO-PHASE GAS FLOATATION SPECTROPHOTOMETRIC DETERMINATION OF TRACE TETRACYCLINE IN ENVIRONMENTAL WATER SAMPLE

    Institute of Scientific and Technical Information of China (English)

    HOU Yanmin; YAN Yongsheng; LI Chunxiang; ZHAO Xiaojun; WANG Liang

    2008-01-01

    A green method for separating and enriching trace tetracycline (TC) in environment water by Aqueous Two-phase Gas Fioatation Spectrophotometry has been proposed, the principium was discussed.In this paper, the hydrophobic complex composed of Mg(Ⅱ) and TC was floated into organic phase under the optimal conditions: pH=10, the floatatlon equipment is home-made, n-propyl alcohol as the organic solvent, sodium chloride as the separating phase reagent.The data were obtained by spectrophotometry after floatatlon; The linear regression ,equation is A=2.33×105 C(mol/L)+0.2179, linear range is from 3.77×107mol/L to 6.32×105mol/L, respectively, with the correlation coefficient (r) better than 0.9997, relative recoveries is 99.7% to 100.3%, limit of detection was 4.29×10-8mol/L, The method can be applied to analyse the trace TC in water sample, the result is better.

  20. A TWO-PHASE APPROACH TO FUZZY SYSTEM IDENTIFICATION

    Institute of Scientific and Technical Information of China (English)

    Ta-Wei HUNG; Shu-Cherng FANG; Henry L.W.NUTTLE

    2003-01-01

    A two-phase approach to fuzzy system identification is proposed. The first phase produces a baseline design to identify a prototype fuzzy system for a target system from a coIlection of input-output data pairs. It uses two easily implemented clustering techniques: the subtractive clustering method and the fuzzy c-means (FCM) clustering algorithm. The second phase (fine tuning)is executed to adjust the parameters identified in the baseline design. This phase uses the steepest descent and recursive least-squares estimation methods. The proposed approach is validated by applying it to both a function approximation type of problem and a classification type of problem. An analysis of the learning behavior of the proposed approach for the two test problems is conducted for further confirmation.

  1. Effects of Hydrophobicity of Ethylene Oxide-Propylene Oxide Copolymers on Phase Diagrams of Aqueous Two-Phase Systems and Partition Behaviors of Cephalexin and 7-Aminodeacetoxicephalos Doranic Acid

    Institute of Scientific and Technical Information of China (English)

    朱建航; 魏东芝; 曹学君

    2002-01-01

    A series of ethylene oxide (EO)-propylene oxide (PO) random co-polymers (EOPO) were used to formaqueous two-phase systems (ATPS) with ammonium sulfate. Effects of EOPO's properties on the phase separationbehaviors and on the partition of cephalexin and 7-aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass and molar ratio of EO to PO of EOPO could greatly influence partition behaviorsof cephalexin and 7-ADCA as well as the binodal curve of ATPS. With the increase of molar mass of co-polymeror the decrease of molar ratio of EO to PO, the critical point of ATPS decreased, the binodal curve became moreasymmetry, and both cephalexin and 7-ADCA followed the same tendency to partition into the polymer-poorbottom phase. The experimental results shows that it is feasible to partition cephalexin and 7-ADCA in either thepolymer-rich top phase or the polymer-poor bottom phase by choosing a specific phase-forming EOPO.

  2. Aqueous two-phase flotation for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10.

    Science.gov (United States)

    Md Sidek, Nurul Lyana; Tan, Joo Shun; Abbasiliasi, Sahar; Wong, Fadzlie Wong Faizal; Mustafa, Shuhaimi; Ariff, Arbakariya B

    2016-08-01

    An aqueous two-phase flotation (ATPF) system based on polyethylene glycol (PEG) and sodium citrate (NaNO3C6H5O7·2H2O) was considered for primary recovery of bacteriocin-like inhibitory substance (BLIS) from Pediococcus acidilactici Kp10. The effects of ATPF parameters namely phase composition, tie-line length (TLL), volume ratio between the two phases (VR), amount of crude load (CL), pH, nitrogen gas flow rate (FR) and flotation time (FT) on the performance of recovery were evaluated. BLIS was mainly concentrated into the upper PEG-rich phase in all systems tested so far. The optimum conditions for BLIS purification, which composed of PEG 8000/sodium citrate, were: TLL of 42.6, VR of 0.4, CL of 22% (w/w), pH 7, average FT of 30min and FR of 20mL/min. BLIS was partially purified up to 5.9-fold with a separation efficiency of 99% under this optimal conditions. A maximum yield of BLIS activity of about 70.3% was recovered in the PEG phase. The BLIS from the top phase was successfully recovered with a single band in SDS-gel with molecular weight of about 10-15kDa. ATPF was found to be an effective technique for the recovery of BLIS from the fermentation broth of P. acidilactici Kp10.

  3. Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis.

    Science.gov (United States)

    Chethana, S; Nayak, Chetan A; Madhusudhan, M C; Raghavarao, K S M S

    2015-04-01

    C-phycocyanin, a natural food colorant, is gaining importance worldwide due to its several medical and pharmaceutical applications. In the present study, aqueous two-phase extraction was shown to be an attractive alternative for the downstream processing of C-phycocyanin from Spirulina platensis. By employing differential partitioning, C-phycocyanin selectively partitioned to the polymer rich (top) phase in concentrated form and contaminant proteins to the salt rich (bottom) phase. This resulted in an increase in the product purity (without losing much of the yield) in a single step without the need of multiple processing steps. Effect of process parameters such as molecular weight, tie line length, phase volume ratio, concentration of phase components on the partitioning behavior of C-phycocyanin was studied. The results were explained based on relative free volume of the phase systems. C-phycocyanin with a purity of 4.32 and yield of about 79 % was obtained at the standardized conditions.

  4. Transient thermohydraulic modeling of two-phase fluid systems

    Science.gov (United States)

    Blet, N.; Delalandre, N.; Ayel, V.; Bertin, Y.; Romestant, C.; Platel, V.

    2012-11-01

    This paper presents a transient thermohydraulic modeling, initially developed for a capillary pumped loop in gravitational applications, but also possibly suitable for all kinds of two-phase fluid systems. Using finite volumes method, it is based on Navier-Stokes equations for transcribing fluid mechanical aspects. The main feature of this 1D-model is based on a network representation by analogy with electrical. This paper also proposes a parametric study of a counterflow condenser following the sensitivity to inlet mass flow rate and cold source temperature. The comparison between modeling results and experimental data highlights a good numerical evaluation of temperatures. Furthermore, the model is able to represent a pretty good dynamic evolution of hydraulic variables.

  5. An automated two-phase system for hydrogel microbead production.

    Science.gov (United States)

    Coutinho, Daniela F; Ahari, Amir F; Kachouie, Nezamoddin N; Gomes, Manuela E; Neves, Nuno M; Reis, Rui L; Khademhosseini, Ali

    2012-09-01

    Polymeric beads have been used for protection and delivery of bioactive materials, such as drugs and cells, for different biomedical applications. Here, we present a generic two-phase system for the production of polymeric microbeads of gellan gum or alginate, based on a combination of in situ polymerization and phase separation. Polymer droplets, dispensed using a syringe pump, formed polymeric microbeads while passing through a hydrophobic phase. These were then crosslinked, and thus stabilized, in a hydrophilic phase as they crossed through the hydrophobic-hydrophilic interface. The system can be adapted to different applications by replacing the bioactive material and the hydrophobic and/or the hydrophilic phases. The size of the microbeads was dependent on the system parameters, such as needle size and solution flow rate. The size and morphology of the microbeads produced by the proposed system were uniform, when parameters were kept constant. This system was successfully used for generating polymeric microbeads with encapsulated fluorescent beads, cell suspensions and cell aggregates proving its ability for generating bioactive carriers that can potentially be used for drug delivery and cell therapy.

  6. Progress on the study of mechanism and partition behavior of papain in affinity aqueous two-phase systems%木瓜蛋白酶在亲和双水相系统中的分配行为及机制研究进展

    Institute of Scientific and Technical Information of China (English)

    张海德; 王伟涛; 蒋欣欣

    2013-01-01

    The separation methods of papain such as ultrafiltration, salt deposition, solvent extraction, affinity chromatography, aqueous two - phase extraction, affinity aqueous two-phase extraction and so on are evaluated. High purity papain can be expected to obtain by metal chelating affinity aqueous two-phase system (ATPS) technology, but the mechanism and partition behavior of papain in this system are not clear. It is important to study the interactions between papain and different metal ion affinity phase-separating agent, the adsorption kinetics model of papain in ATPS and the physical structure characterization and molecular simulation of the enzyme affinity adsorbent. Metal chelating affinity ATPS technology based on papain separation, will vigorously promote the development of papain industrialization and it has stimulative effect on the development of aqueous two-phase extraction technology.%  本文查阅相关文献,对木瓜蛋白酶传统提取方法(超滤法、盐析法、有机溶剂法、亲和层析法)及一些新兴的提取方法如双水相萃取法、亲和双水相法作了简要综述。利用金属螯合亲和双水相分配系统,能有效获得高纯度木瓜蛋白酶,但木瓜蛋白酶与亲和成相剂的相互作用及其分配行为与机制尚不清楚。研究木瓜蛋白酶与不同金属离子亲和成相剂之间的相互作用和吸附动力学模型;对酶的亲和结合物进行物理表征和分子模拟。阐明木瓜蛋白酶与不同金属离子亲和成相剂之间的亲和作用原理。将双水相萃取技术和亲和分配技术相融合,提出基于木瓜蛋白酶的金属螯合亲和双水相分配技术,该技术有望大力推动木瓜蛋白酶工业化高效制备的发展。

  7. Determination of Sudan I-IV in candy using ionic liquid/anionic surfactant aqueous two-phase extraction coupled with high-performance liquid chromatography.

    Science.gov (United States)

    Yu, Wei; Liu, Zhongling; Li, Qiang; Zhang, Hanqi; Yu, Yong

    2015-04-15

    Ionic liquid/anionic surfactant aqueous two-phase system was developed and applied for the extraction of Sudan I-IV. High-performance liquid chromatography was applied to the determination of the analytes. The aqueous two-phase system (ATPS) was formed in the present of C4[MIM]BF4, sodium dodecyl benzene sulphonate and (NH4)2SO4. The parameters affecting the extraction efficiency, such as volume of ionic liquid, amount of sodium dodecyl benzene sulphonate, ionic strength, pH value of system, extraction time and temperature were investigated. The limits of detection for Sudan I, II, III and IV were 5.45, 4.66, 3.68, 4.20 μg kg(-1), respectively. When the present method was applied to the analysis of candy samples, the recoveries of the analytes ranged from 82.3% to 112.1% and relative standard deviations were lower than 7.41%.

  8. Degradation of chloramphenicol in aqueous solution using ozone oxidation in a two-phase system of water/loaded ozone organic solvent%水/载O3有机溶剂两相臭氧氧化法降解水体中氯霉素

    Institute of Scientific and Technical Information of China (English)

    李时银; 史震宇; 陆继来; 邹敏; 张利民

    2011-01-01

    Using a two-phase system of water/perfluorodecalin organic solvent loaded ozone, degradation of chloramphenicol in aqueous solution was conducted. Due to perfluorodecalin has a high ozone stability and solubility, chloramphenicol was used to examine the efficiency of the two-phase ozonation system. During the experiments, effects of initial pH and free radical scavenger on the removal rate of chloramphenicol were investigated respectively. The results showed that the efficiency of two-phase ozonation was increased with the increase of initial pH in water. It was also observed that free radical scavenger had no significant negative effect in two-phase ozonation.%以水/载O3有机溶剂(全氟萘烷)为新型两相臭氧氧化体系,研究了臭氧在全氟萘烷中的溶解度和稳定性,并考察了pH、NaHCO3和叔丁醇对两相体系中臭氧稳定性的影响;以水体中氯霉素为研究对象,考察了初始pH值和自由基抑制剂对两相臭氧氧化体系降解氯霉素效果的影响.结果表明:初始水相pH增大有利于氯霉素的降解;自由基抑制剂对两相体系氧化降解氯霉素影响不显著.

  9. Integrated downstream processing of lactoperoxidase from milk whey involving aqueous two-phase extraction and ultrasound-assisted ultrafiltration.

    Science.gov (United States)

    Nandini, K E; Rastogi, Navin K

    2011-01-01

    The present work involves the adoption of an integrated approach for the purification of lactoperoxidase from milk whey by coupling aqueous two-phase extraction (ATPE) with ultrasound-assisted ultrafiltration. The effect of system parameters of ATPE such as type of phase system, polyethylene glycol (PEG) molecular mass, system pH, tie line length and phase volume ratio was evaluated so as to obtain differential partitioning of contaminant proteins and lactoperoxidase in top and bottom phases, respectively. PEG 6000-potassium phosphate system was found to be suitable for the maximum activity recovery of lactoperoxidase 150.70% leading to 2.31-fold purity. Further, concentration and purification of enzyme was attempted using ultrafiltration. The activity recovery and purification factor achieved after ultrafiltration were 149.85% and 3.53-fold, respectively. To optimise productivity and cost-effectiveness of integrated process, influence of ultrasound for the enhancement of permeate flux during ultrafiltration was also investigated. Intermittent use of ultrasound along with stirring (2 min acoustic and 2 min stirring) resulted in increased permeate flux from 0.94 to 2.18 l/m(2) h in comparison to the ultrafiltration without ultrasound. The use of ultrasound during ultrafiltration resulted in increase in flux, but there was no significant change in activity recovery and purification factor. The integrated approach involving ATPE and ultrafiltration may prove to be a feasible method for the downstream processing of lactoperoxidase from milk whey.

  10. Partition behaviors of oxytetracycline hydrochloride (OTC) in small molecule alcohol/salt binary aqueous two-phase system%小分子醇/盐二元双水相体系中盐酸土霉素的分配行为

    Institute of Scientific and Technical Information of China (English)

    关卫省; 柴丽; 韩娟

    2012-01-01

    Based on the aqueous two-phase system,a new method of using ethanol and re-propanol with sodium dihydrogen phosphate binary aqueous two-phase system for extracting of oxytetracycline hydrochloride (OTC) was set up. The influence factors on partition behaviors of oxytetracyeline hydrochloride (OTC) were studied,including the system composition,type and amount of salts,the pH value,the extraction temperature, and standing time after centrifuge. The results showed that the binary aqueous two-phase system can be used to study the distribution of antibiotics. When the system was ethanol and n-pro-panol, NaH2 PO4 concentration of 48% , the pH value was 4~ 5, temperature was 25℃, and standing 12 h ,the partition coefficient of oxytetracycline hydrochloride ( OTC) was 21.95, and extraction rate was 86.09%.%在双水相体系研究的基础上,建立了乙醇与正丙醇和磷酸二氢钠形成的二元双水相体系萃取盐酸土霉素的新方法,考察了小分子醇的用量、盐种类和浓度、pH值、温度以及静置时间对盐酸土霉素分配行为的影响.结果表明,小分子醇/盐二元双水相体系可用于抗生素分配行为的研究,其中体系组成为乙醇和正丙醇,磷酸二氢钠浓度在48%,pH值在4~5,温度25℃,以及静置12 h左右,盐酸土霉素在该二元双水相体系中的分配系数达到21.95,萃取率达86.09%.

  11. ‘Heat-Treatment Aqueous Two Phase System’ for Purification of Serine Protease from Kesinai (Streblus asper Leaves

    Directory of Open Access Journals (Sweden)

    Shuhaimi Mustafa

    2011-12-01

    Full Text Available A ‘Heat treatment aqueous two phase system’ was employed for the first time to purify serine protease from kesinai (Streblus asper leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEG 4000, 6000 and 8000 at concentrations of 8, 16 and 21% (w/w as well as salts (Na-citrate, MgSO4 and K2HPO4 at concentrations of 12, 15, 18% (w/w on serine protease partition behavior were studied. Optimum conditions for serine protease purification were achieved in the PEG-rich phase with composition of 16% PEG6000-15% MgSO4. Also, thermal treatment of kesinai leaves at 55 °C for 15 min resulted in higher purity and recovery yield compared to the non-heat treatment sample. Furthermore, this study investigated the effects of various concentrations of NaCl addition (2, 4, 6 and 8% w/w and different pH (4, 7 and 9 on the optimization of the system to obtain high yields of the enzyme. The recovery of serine protease was significantly enhanced in the presence of 4% (w/w of NaCl at pH 7.0. Based on this system, the purification factor was increased 14.4 fold and achieved a high yield of 96.7%.

  12. A Simple Approach to Characterize Gas-Aqueous Liquid Two-phase Flow Configuration Based on Discrete Solid-Liquid Contact Electrification.

    Science.gov (United States)

    Choi, Dongwhi; Lee, Donghyeon; Kim, Dong Sung

    2015-10-14

    In this study, we first suggest a simple approach to characterize configuration of gas-aqueous liquid two-phase flow based on discrete solid-liquid contact electrification, which is a newly defined concept as a sequential process of solid-liquid contact and successive detachment of the contact liquid from the solid surface. This approach exhibits several advantages such as simple operation, precise measurement, and cost-effectiveness. By using electric potential that is spontaneously generated by discrete solid-liquid contact electrification, the configurations of the gas-aqueous liquid two-phase flow such as size of a gas slug and flow rate are precisely characterized. According to the experimental and numerical analyses on parameters that affect electric potential, gas slugs have been verified to behave similarly to point electric charges when the measuring point of the electric potential is far enough from the gas slug. In addition, the configuration of the gas-aqueous liquid two-phase microfluidic system with multiple gas slugs is also characterized by using the presented approach. For a proof-of-concept demonstration of using the proposed approach in a self-triggered sensor, a gas slug detector with a counter system is developed to show its practicality and applicability.

  13. Magnetic self-assembly of microparticle clusters in an aqueous two-phase microfluidic cross-flow

    Science.gov (United States)

    Abbasi, Niki; Jones, Steven G.; Moon, Byeong-Ui; Tsai, Scott S. H.

    2015-11-01

    We present a technique that self-assembles paramagnetic microparticles on the interface of aqueous two-phase system (ATPS) fluids in a microfluidic cross-flow. A co-flow of the ATPS is formed in the microfluidic cross channel as the flows of a dilute dextran (DEX) phase, along with a flow-focused particle suspension, converges with a dilute polyethylene glycol (PEG) phase. The microparticles arrive at the liquid-liquid interface and self-assemble into particle clusters due to forces on the particles from an applied external magnetic field gradient, and the interfacial tension of the ATPS. The microparticles form clusters at the interface, and once the cluster size grows to a critical value, the cluster passes through the interface. We control the size of the self-assembled clusters, as they pass through the interface, by varying the strength of the applied magnetic field gradient and the ATPS interfacial tension. We observe rich assembly dynamics, from the formation of Pickering emulsions to clusters that are completely encapsulated inside DEX phase droplets. We anticipate that this microparticle self-assembly method may have important biotechnological applications that require the controlled assembly of cells into clusters.

  14. Individual extraction constants of some univalent anions in the two-phase water-phenyltrifluoromethyl sulfone system.

    Science.gov (United States)

    Makrlík, Emanuel; Selucký, Pavel; Vaňura, Petr

    2011-12-01

    From extraction experiments and g-activity measurements, the extraction constants corresponding to the general equilibrium Cs+(aq) + A- (aq) Cs+(org) + A- (org) taking place in the two-phase water-phenyltrifluoromethyl sulfone (FS 13) system (A-= I-, ClO4-, MnO4-, Br-3, I-3, picrate, tetraphenylborate (BPh-4); aq = aqueous phase, org = FS 13 phase) were evaluated. Furthermore, the individual extraction constants of these 7 anions in the mentioned two-phase system were calculated; they were found to increase in the series of I-< ClO4- < Br-3 < MnO4-, picrate < I-3 < BPh-4.

  15. Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms.

    Science.gov (United States)

    Bentley, Fiona K; Melis, Anastasios

    2012-01-01

    Photosynthesis for the generation of fuels and chemicals from cyanobacteria and microalgae offers the promise of a single host organism acting both as photocatalyst and processor, performing sunlight absorption and utilization, as well as CO(2) assimilation and conversion into product. However, there is a need to develop methods for generating, sequestering, and trapping such bio-products in an efficient and cost-effective manner that is suitable for industrial scale-up and exploitation. A sealed gaseous/aqueous two-phase photobioreactor was designed and applied for the photosynthetic generation of volatile isoprene (C(5)H(8)) hydrocarbons, which operates on the principle of spontaneous diffusion of CO(2) from the gaseous headspace into the microalgal or cyanobacterial-containing aqueous phase, followed by photosynthetic CO(2) assimilation and isoprene production by the transgenic microorganisms. Volatile isoprene hydrocarbons were emitted from the aqueous phase and were sequestered into the gaseous headspace. Periodic replacement (flushing) of the isoprene (C(5)H(8)) and oxygen (O(2)) content of the gaseous headspace with CO(2) allowed for the simultaneous harvesting of the photoproducts and replenishment of the CO(2) supply in the gaseous headspace. Reduction in practice of the gaseous/aqueous two-phase photobioreactor is offered in this work with a fed-batch and a semi-continuous culturing system using Synechocystis sp. PCC 6803 heterologously expressing the Pueraria montana (kudzu) isoprene synthase (IspS) gene. Constitutive isoprene production was observed over 192 h of experimentation, coupled with cyanobacterial biomass accumulation. The diffusion-based process in gaseous/aqueous two-phase photobioreactors has the potential to be applied to other high-value photosynthetically derived volatile molecules, emanating from a variety of photosynthetic microorganisms.

  16. 超声辅助异丙醇/(NH4)2SO4双水相体系提取金银花中总黄酮%Extraction of Total Flavonoids fromHoneysuckle with Isopropano/Ammonium Sulfate Aqueous Two-phase System by Ultrasonic-assisted

    Institute of Scientific and Technical Information of China (English)

    程志发; 马燮; 毛华剑

    2016-01-01

    Aqueous two-phase system was constructed with alcohols / ammonium sulfate / water.The extraction operating conditions of total flavonoids fromhoneysuckle by ultrasonic-assisted were investigated. The experiments results showed that the total flavnoids mostly accumulated in the top phase.It was found that the concentration of isopropanol and ammonium sulfatehad greater effect on aqueous two-phase extraction. The appropriate conditions were as followed: the quality percentage of isopropanol was 30% and the quality percentage of ammonium sulfate was 25%, ultrasonic treat time was 20 min. Under these conditions, the distribution coefficient K was 29.4 and the extraction ratio was 94.8%.%采用异丙醇/硫酸铵/水构建双水相体系,研究在超声波作用下操作条件对金银花中总黄酮萃取效果的影响。实验结果表明,金银花总黄酮主要分布在上相,双水相体系的组成(异丙醇与硫酸铵的浓度)对萃取效果的影响较大。当异丙醇质量分数为30%、硫酸铵质量分数为25%时,常温下超声处理20min,分配系数为29.4,总黄酮的萃取率为94.8%。

  17. Gibbs free energy of transfer of a methylene group on {l_brace}UCON + (sodium or potassium) phosphate salts{r_brace} aqueous two-phase systems: Hydrophobicity effects

    Energy Technology Data Exchange (ETDEWEB)

    Silverio, Sara C. [LSRE - Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n. 4200-465 Porto (Portugal); IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga (Portugal); Rodriguez, Oscar [LSRE - Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n. 4200-465 Porto. Portugal (Portugal); Teixeira, Jose A. [IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga (Portugal); Macedo, Eugenia A., E-mail: eamacedo@fe.up.p [LSRE - Laboratory of Separation and Reaction Engineering, Associate Laboratory LSRE/LCM, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias s/n. 4200-465 Porto (Portugal)

    2010-08-15

    The Gibbs free energy of transfer of a suitable hydrophobic probe can be regarded as a measure of the relative hydrophobicity of the different phases. The methylene group (CH{sub 2}) can be considered hydrophobic, and thus be a suitable probe for hydrophobicity. In this work, the partition coefficients of a series of five dinitrophenylated-amino acids were experimentally determined, at 23 {sup o}C, in three different tie-lines of the biphasic systems: (UCON + K{sub 2}HPO{sub 4}), (UCON + potassium phosphate buffer, pH 7), (UCON + KH{sub 2}PO{sub 4}), (UCON + Na{sub 2}HPO{sub 4}), (UCON + sodium phosphate buffer, pH 7), and (UCON + NaH{sub 2}PO{sub 4}). The Gibbs free energy of transfer of CH{sub 2} units were calculated from the partition coefficients and used to compare the relative hydrophobicity of the equilibrium phases. The largest relative hydrophobicity was found for the ATPS formed by dihydrogen phosphate salts.

  18. Effect of biochar addition on hydrogen and methane production in two-phase anaerobic digestion of aqueous carbohydrates food waste.

    Science.gov (United States)

    Sunyoto, Nimas M S; Zhu, Mingming; Zhang, Zhezi; Zhang, Dongke

    2016-11-01

    Effect of biochar addition on hydrogen and methane production in two-phase anaerobic digestion of aqueous carbohydrates was studied using bench-scale bioreactors. The cultures with biochar additions were placed in 100ml reactors and incubated at 35°C and pH 5 for hydrogen production. The residual cultures were then used for methane production, incubated at 35°C and pH 7. Daily yields of hydrogen and methane and weekly yield of volatile fatty acids (VFA) were measured. The hydrogen and methane production potentials, rate and lag phases of the two phases were analysed using the Gompertz model. The results showed that biochar addition increased the maximum production rates of hydrogen by 32.5% and methane 41.6%, improved hydrogen yield by 31.0% and methane 10.0%, and shortened the lag phases in the two phases by 36.0% and 41.0%, respectively. Biochar addition also enhanced VFA generation during hydrogen production and VFA degradation in methane production.

  19. Animal-cell culture in aqueous two-phase systems.

    NARCIS (Netherlands)

    Zijlstra, G.M.

    1998-01-01

    In current industrial biotechnology, animal-cell culture is an important source of therapeutic protein products. The conventional animal-cell production processes, however, include many unit operations as part of the fermentation and downstream processing strategy. The research described in this the

  20. Aqueous two-phase (PEG4000/Na2SO4) extraction and characterization of an acid invertase from potato tuber (Solanum tuberosum).

    Science.gov (United States)

    Yuzugullu, Yonca; Duman, Yonca Avcı

    2015-01-01

    Invertases are key metabolic enzymes that catalyze irreversible hydrolysis of sucrose into fructose and glucose. Plant invertases have essential roles in carbohydrate metabolism, plant development, and stress responses. To study their isolation and purification from potato, an attractive system useful for the separation of biological molecules, an aqueous two-phase system, was used. The influence of various system parameters such as type of phase-forming salts, polyethylene glycol (PEG) molecular mass, salt, and polymer concentration was investigated to obtain the highest recovery of enzyme. The PEG4000 (12.5%, w/w)/Na2SO4(15%, w/w) system was found to be ideal for partitioning invertase into the bottom salt-rich phase. The addition of 3% MnSO4 (w/w) at pH 5.0 increased the purity by 5.11-fold with the recovered activity of 197%. The Km and Vmax on sucrose were 3.95 mM and 0.143 U mL(-1) min(-1), respectively. Our data confirmed that the PEG4000/Na2SO4 aqueous two-phase system combined with the presence of MnSO4 offers a low-cost purification of invertase from readily available potato tuber in a single step. The biochemical characteristics of temperature and pH stability for potato invertase prepared from an ATPS make the enzyme a good candidate for its potential use in many research and industrial applications.

  1. A two-phase system call arguments attribute analyzing method

    Institute of Scientific and Technical Information of China (English)

    LI Hong-jiao; LI Jian-hua

    2008-01-01

    To detect more attacks aiming at key security data in program behavior-based anomaly detection, the data flow properties were formulated as unary and binary relations on system call arguments. A new method named two-phrase analysis (2PA) is designed to analyze the efficient relation dependency, and its description as well as advantages are discussed. During the phase of static analysis, a dependency graph was constructed according to the program's data dependency graph, which was used in the phase of dynamic learning to learn specified binary relations. The constructed dependency graph only stores the information of related arguments and events, thus improves the efficiency of the learning algorithm and reduces the size of learned relation de-pendencies. Performance evaluations show that the new method is more efficient than existing methods.

  2. Enzymatic hydrolysis of penicillin in mixed ionic liquids/water two-phase system.

    Science.gov (United States)

    Jiang, Yangyang; Xia, Hansong; Guo, Chen; Mahmood, Iram; Liu, Huizhou

    2007-01-01

    In this paper, an integrated process involving the mixed ionic liquids/water two-phase system (MILWS) is proposed to improve the efficiency for enzymatic hydrolysis of penicillin G. First, hydrophilic [C4mim]BF4 (1-butyl-3-methylimidazolium tetrafluoraborate) and NaH2PO4 salt form an ionic liquids aqueous two-phase system (ILATPS), which could extract penicillin from its fermentation broth efficiently. Second, a hydrophobic [C4mim]PF6 (1-butyl-3-methylimidazolium hexafluoraphosphate) is introduced into the ionic liquids-rich phase of ILATPS containing penicillin and converses it into MILWS. Penicillin is hydrolyzed by penicillin acylase in the water phase of MILWS at pH 5. The byproduct phenylacetic acid (PAA) is partitioned into the ionic liquids mixture phase, while the intended product 6-aminopenicillanic acid (6-APA) is precipitated at this pH. In comparison with a similar butyl acetate/water system (BAWS) at pH 4, MILWS exhibits two advantages. (1) The selectivity between PAA and penicillin is greatly optimized at pH 5 by varying the mole ratio of [C4mim]PF6/[C4mim]BF4 in MILWS, whereas in BAWS the unalterable nature of the organic solvent restricts the optimized pH for maximum selectivity between PAA and penicillin at pH 4. (2) The pH for 6-APA precipitation in BAWS is 4, whereas it shifts to pH 5 in MILWS due to the complexation between negatively charged 6-APA and the cationic surface of the ionic liquids micelle. As a result, the removal of the two products from the enzyme sphere at relatively high pH is permitted in MILWS, which is beneficial for enzymatic activity and stability in comparison with the acidic pH 4 environment in BAWS.

  3. 聚乙二醇-硫酸铵双水相体系萃取螺旋藻藻蓝蛋白的研究%The extraction of C-phycocyanin from Spirulina using aqueous two- phase system composed of PEG and ammonium sulfate

    Institute of Scientific and Technical Information of China (English)

    于淑坤; 岳思君; 田露; 苏建宇

    2012-01-01

    对聚乙二醇一硫酸铵双水相体系直接从螺旋藻破碎液中萃取藻蓝蛋白的技术进行了研究。结果表明,对于藻蓝蛋白含量为0.282mg/mL的螺旋藻细胞破碎液,10%PEG2000与20%的硫酸铵双水相体系萃取藻蓝蛋白的效果最佳,经2次革取,藻蓝蛋白总革取率为96.1,藻蓝蛋白纯度达到1.2。%The technology of extracting C-phycocyanin from Spirulina by using a aqueous two-phase system composed of PEG and ammonium sulfate was studied in this paper. The results showed that the optimum conditions of extraction were 10 percent of PEG2000 and 20 percent of (NH4)2SO4. The total extraction rate of C-phycocyanin was reached to 96.1 percent and the purity of C-phycocyanin was as high as 1.2.

  4. Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system Isolamento de Trichosporon spp produtor de lipase e extração enzimática pelo sistema bifásico aquoso

    Directory of Open Access Journals (Sweden)

    Juliana A. Santos

    2007-03-01

    Full Text Available A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts showed an elevated recuperation (99.8% of enzymatic activity in the PEG phase.Uma levedura produtora de lipase isolada de queijo coalho e identificada como Trichosporon spp produziu 7,3 U/mL (59,3 U/µg após 72h de cultivo. A lipase mostrou atividade ótima em pH 7,0-8,0 e temperatura ótima entre 45-50ºC. Extração pelo sistema PEG - sais de fosfato apresentou 99,8% de recuperação da atividade enzimática na fase PEG.

  5. Symmetrical components and power analysis for a two-phase microgrid system

    DEFF Research Database (Denmark)

    Alibeik, M.; Santos Jr., E. C. dos; Blaabjerg, Frede

    2014-01-01

    This paper presents a mathematical model for the symmetrical components and power analysis of a new microgrid system consisting of three wires and two voltages in quadrature, which is designated as a two-phase microgrid. The two-phase microgrid presents the following advantages: 1) constant power...

  6. Cells of Candida utilis for in vitro (R)-phenylacetylcarbinol production in an aqueous/octanol two-phase reactor.

    Science.gov (United States)

    Rosche, Bettina; Breuer, Michael; Hauer, Bernhard; Rogers, Peter L

    2005-04-01

    (R)-Phenylacetylcarbinol (PAC), a pharmaceutical precursor, was produced from benzaldehyde and pyruvate by pyruvate decarboxylase (PDC) of Candida utilis in an aqueous/organic two-phase emulsion reactor. When the partially purified enzyme in this previously established in vitro process was replaced with C. utilis cells and the temperature was increased from 4 to 21 degrees C, a screen of several 1-alcohols (C4-C9) confirmed the suitability of 1-octanol as the organic phase. Benzyl alcohol, the major by-product in the commercial in vivo conversion of benzaldehyde and sugar to PAC by Saccharomyces cerevisiae, was not formed. With a phase volume ratio of 1:1 and 5.6 g C. utilis l-1 (PDC activity 2.5 U ml-1), PAC levels of 103 g l-1 in the octanol phase and 12.8 g l-1 in the aqueous phase were produced in 15 h at 21 degrees C. In comparison to our previously published process with partially purified PDC in an aqueous/octanol emulsion at 4 degrees C, PAC was produced at a 4-times increased specific rate (1.54 versus 0.39 mg U-1 h-1) with simplified catalyst production and reduced cooling cost. Compared to traditional in vivo whole cell PAC production, the yield on benzaldehyde was 26% higher, the product concentration increased 3.9-fold (or 6.9-fold based on the organic phase), the productivity improved 3.1-fold (3.9 g l-1 h-1) and the catalyst was 6.9-fold more efficient (PAC/dry cell mass 10.3 g g-1).

  7. Drift flux modelling for a two-phase system in a flotation column

    Energy Technology Data Exchange (ETDEWEB)

    Vandenberghe, J.; Choung, J.; Xu, Z.; Masliyah, J. [Alberta Univ., Edmonton, AB (Canada). Dept. of Chemical and Materials Engineering

    2005-04-01

    Mineral and coal industries use flotation columns to improve recovery, upgrade products, save energy and reduce scale. Flotation columns are a proven process equipment that are adaptable to computer process control and offer flexible operation. Once gas is introduced to the bottom of the flotation column, bubbles form and rise throughout the pulp. Hydrophobic particles in the pulp attache to the bubbles, making the density of the bubble particle aggregates less than that of the medium. The bubble zone and the froth zone are the 2 main zones of a flotation column. This study applied the drift flux analysis to a wide range of operating conditions of a two-phase system column in order develop a better empirical relation that can predict the bubbly and froth zone characteristics. Tests were performed with process water taken from a bitumen extraction process at Syncrude Canada Limited with aqueous solutions having low concentrations of methyl isobutyl carbinol at 7.8 or 15.5 ppm. A new correlation for the drift flux system characteristic curve was discovered in this study. The new correlation is applicable for a bubble Reynolds number range of 5 to 70. The numerical equation for this correlation was presented. The bubble and froth zones have different hydrodynamics. Therefore, the method for calculating the Reynolds number in the bubble zone may not be suitable for the froth zone. Therefore, a new equation was proposed to accommodate a wider operating range. 16 refs., 1 tab., 13 figs.

  8. NaCl-丙醇双水相体系分离富集草甘膦生产废水中草甘膦铵盐%Separation and enrichment of glyphosate from glyphosate wastewater by NaCl/propanol aqueous two-phase system

    Institute of Scientific and Technical Information of China (English)

    余龙; 余凤; 陈俊

    2013-01-01

    By using aqueous two-phase system,which is formed by saturating NaCl that is in the glyphosate wastewater and propanol as a system to separate and enrich part of residues ammonium glyphosae in the glyphosate wastewater,the problems of resources recycling and recovery can be solved efficiently.The best extraction conditions can be determined by analyzing four factors:kinds and amounts of surfactant,pH,the amount of NaCl and propanol.The last step is to verify the scheme by simulation.The result indicates that the treatment of wastewater is ideal under this scheme which meets the requirements for industrialization and realizes 90.7% high recovery rate.%利用草甘膦生产废水中接近饱和的NaC1与丙醇形成的双水相体系分离富集废水中部分残留的草甘膦铵盐,达到了资源循环利用和资源的高效回收.通过分析表面活性剂种类及用量,pH,丙醇质量分数和NaC1质量分数4个因素确定了最佳萃取条件.最后对该方案进行仿真验证,表明利用该方案对实际废水的处理比较理想,实现了90.7%的高回收率,完全符合工业化要求.

  9. Living between two worlds: two-phase culture systems for producing plant secondary metabolites.

    Science.gov (United States)

    Malik, Sonia; Hossein Mirjalili, Mohammad; Fett-Neto, Arthur Germano; Mazzafera, Paulo; Bonfill, Mercedes

    2013-03-01

    The two-phase culture system is an important in vitro strategy to increase the production of secondary metabolites (SMs) by providing an enhanced release of these compounds from plant cells. Whereas the first phase supports cell growth, the second phase provides an additional site or acts as a metabolic sink for the accumulation of SMs and also reduces feedback inhibition. This review is focused on several aspects of the two-phase culture system and aims to show the diverse possibilities of employing this technique for the in vitro production of SMs from plant cells. Depending on the material used in the secondary phase, two-phase culture systems can be broadly categorised as liquid-liquid or liquid-solid. The choice of material for the second phase depends on the type of compound to be recovered and the compatibility with the other phase. Different factors affecting the efficiency of two-phase culture systems include the choice of material for the secondary phase, its concentration, volume, and time of addition. Factors such as cell elicitation, immobilization, and permeabilization, have been suggested as important strategies to make the two-phase culture system practically reliable on a commercial scale. Since there are many possibilities for designing a two-phase system, more detailed studies are needed to broaden the range of secondary phases compatible with the various plant species producing SMs with potential applications, mainly in the food and pharmacology industries.

  10. Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF).

    Science.gov (United States)

    Li, Xuan; Kim, Tae Hyun; Nghiem, Nhuan P

    2010-08-01

    An integrated bioconversion process was developed to convert corn stover derived pentose and hexose to ethanol effectively. In this study, corn stover was pretreated by soaking in aqueous ammonia (SAA), which retained glucan ( approximately 100%) and xylan (>80%) in the solids. The pretreated carbohydrates-rich corn stover was converted to ethanol via two-phase simultaneous saccharification and fermentation (TPSSF). This single-reactor process employed sequential simultaneous saccharification and fermentation (SSF), i.e. pentose conversion using recombinant Escherichia coli KO11 in the first phase, followed by hexose conversion with Saccharomyces cerevisiae D5A in the second phase. In the first phase, 88% of xylan digestibility was achieved through the synergistic action of xylanase and endo-glucanase with minimal glucan hydrolysis (10.5%). Overall, the TPSSF using 12-h SAA-treated corn stover resulted in the highest ethanol concentration (22.3g/L), which was equivalent to 84% of the theoretical ethanol yield based on the total carbohydrates (glucan+xylan) in the untreated corn stover.

  11. COUPLING MODEL OF TWO PHASE FLOW IN A FRACTURE-ROCK MATRIX SYSTEM AND ITS STOCHASTIC FEATURE ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    王经明; 李竞生; 高智联; 杨保禹

    1998-01-01

    This study is concerned with developing a two-dimensional two-phase model thatsimulate the movement of non-aqueous phase liquid (NAPL) in a fracture-rock matrix system. Theintrinsic permeability and the fracture aperture are represented in the model via its Karhunen-Loeve expansion. Other parameters and the nodal unknowns, water saturations and waterpressures, are represented by their stochastic spectral expanions. The errors resulting fromtruncation of Karhunen - Loeve and polynomial chaos expansions to a finite number of terms areanalyzed. The eigenvalues of stochastic process is found out for any point in the special domain ofthe problem at any instant in time.

  12. Enantioseparation of Racemic Flurbiprofen by Aqueous Two-Phase Extraction With Binary Chiral Selectors of L-dioctyl Tartrate and L-tryptophan.

    Science.gov (United States)

    Chen, Zhi; Zhang, Wei; Wang, Liping; Fan, Huajun; Wan, Qiang; Wu, Xuehao; Tang, Xunyou; Tang, James Z

    2015-09-01

    A novel method for chiral separation of flurbiprofen enantiomers was developed using aqueous two-phase extraction (ATPE) coupled with biphasic recognition chiral extraction (BRCE). An aqueous two-phase system (ATPS) was used as an extracting solvent which was composed of ethanol (35.0% w/w) and ammonium sulfate (18.0% w/w). The chiral selectors in ATPS for BRCE consideration were L-dioctyl tartrate and L-tryptophan, which were screened from amino acids, β-cyclodextrin derivatives, and L-tartrate esters. Factors such as the amounts of L-dioctyl tartrate and L-tryptophan, pH, flurbiprofen concentration, and the operation temperature were investigated in terms of chiral separation of flurbiprofen enantiomers. The optimum conditions were as follows: L-dioctyl tartrate, 80 mg; L-tryptophan, 40 mg; pH, 4.0; flurbiprofen concentration, 0.10 mmol/L; and temperature, 25 °C. The maximum separation factor α for flurbiprofen enantiomers could reach 2.34. The mechanism of chiral separation of flurbiprofen enantiomers is discussed and studied. The results showed that synergistic extraction has been established by L-dioctyl tartrate and L-tryptophan, which enantioselectively recognized R- and S-enantiomers in top and bottom phases, respectively. Compared to conventional liquid-liquid extraction, ATPE coupled with BRCE possessed higher separation efficiency and enantioselectivity without the use of any other organic solvents. The proposed method is a potential and powerful alternative to conventional extraction for separation of various enantiomers.

  13. Two-phase flow stability structure in a natural circulation system

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Zhiwei [Nuclear Engineering Laboratory Zurich (Switzerland)

    1995-09-01

    The present study reports a numerical analysis of two-phase flow stability structures in a natural circulation system with two parallel, heated channels. The numerical model is derived, based on the Galerkin moving nodal method. This analysis is related to some design options applicable to integral heating reactors with a slightly-boiling operation mode, and is also of general interest to similar facilities. The options include: (1) Symmetric heating and throttling; (2) Asymmetric heating and symmetric throttling; (3) Asymmetric heating and throttling. The oscillation modes for these variants are discussed. Comparisons with the data from the INET two-phase flow stability experiment have qualitatively validated the present analysis.

  14. Design, Modelling and Simulation of Two-Phase Two-Stage Electronic System with Orthogonal Output for Supplying of Two-Phase ASM

    Directory of Open Access Journals (Sweden)

    Michal Prazenica

    2011-01-01

    Full Text Available This paper deals with the two-stage two-phase electronic systems with orthogonal output voltages and currents - DC/AC/AC. Design of two-stage DC/AC/AC high frequency converter with two-phase orthogonal output using single-phase matrix converter is also introduced. Output voltages of them are strongly nonharmonic ones, so they must be pulse-modulated due to requested nearly sinusoidal currents with low total harmonic distortion. Simulation experiment results of matrix converter for both steady and transient states for IM motors are given in the paper, also experimental verification under R-L load, so far. The simulation results confirm a very good time-waveform of the phase current and the system seems to be suitable for low-cost application in automotive/aerospace industries and application with high frequency voltage sources.

  15. Infleunce of pH on the partition of glucose-6-phosphate dehydrogenase and hexokinase in aqueous two-phase system Influência do pH na partição da glicose 6-fosfato desidrogenase e hexoquinase em sistema de duas fases aquosas

    Directory of Open Access Journals (Sweden)

    Daniel Pereira da Silva

    2002-09-01

    Full Text Available Glucose-6-phosphate dehydrogenase (G6PDH and hexokinase (HK are important enzymes used in biochemical and medical studies and in several analytical methods. Aqueous two-phase system (ATPS formed by a polymer solution and an electrolyte solution provides a method for the separation and purification of enzymes with several advantages, including biocompatibility and easy scale up of the process. In this work, the effects of different pH values on the storage stability and partitioning behavior (K, partition coefficient of the enzymes G6PDH and HK from baker's yeast extract were investigated in ATPS. The results, obtained from the 17.5% PEG 400 : 15.0% phosphate system, showed that when the pH was increased from 5.0 to 8.8, the K HK increased 26-fold and the K G6PDH 2.2-fold. In the 20.0% PEG 1500 : 17.5% phosphate system, the K HK and K G6PDH increased 13 and 1.2-fold, when the pH value was increased from 3.8 to 8.8, respectively. This leads to the conclusion that the partition coefficient for both enzymes is favored by high pH values. A statistical analysis of the results was conducted to confirm this conclusion.Glicose-6-fosfato desidrogenase (G6PDH e hexoquinase (HK são importantes enzimas usadas em estudos bioquímicos e médicos e em diversos métodos analíticos. Sistema de duas fases aquosas (SDFA formado por uma solução polimérica e uma solução eletrolítica proporciona um método para separação e purificação de enzimas com diversas vantagens, incluindo biocompatibilidade, que pode ser facilmente escalonado para nível industrial. Neste trabalho, os efeitos de diferentes valores de pH na estabilidade e na partição (K, coeficiente de partição por SDFA das enzimas G6PDH e HK, obtidas através de levedura de panificação, foram investigados. Os resultados, obtidos do sistema constituído por 17,5% de PEG 400 e 15,0% de fosfato, mostraram que com a elevação do pH de 5,0 para 8,8, o K HK aumentou 26 vezes e o K G6PDH 2,2 vezes

  16. Study on the Ultrasound Extraction Coupling with Two-phase Aqueous System in the Extraction of Polyphenols from Mango Nuclear and Its Antioxidantion Activity%超声与双水相体系耦合提取芒果核多酚及活性研究

    Institute of Scientific and Technical Information of China (English)

    高云涛; 付艳丽; 李正全; 蒋进超

    2009-01-01

    利用超声与丙醇-硫酸铵双水相体系耦合对芒果核多酚进行提取分离,通过正交试验法对双水相形成和超声提取条件进行优化,最佳提取条件是:丙醇体积分数60%,超声提取15 min,(NH_4)_2SO_4用量为3 g/L,料液比(g∶mL)为1∶20,在此提取条件下多酚率提取率为6.96%,提取物中植物多酚纯度为37.8%,明显高于回流提取法.提取物对脂质过氧化及诱导红细胞氧化损伤具有良好的抑制作用,最大抑制率分别为66.8%和67.4%,方法简便、条件温和,环境友好.%The ultrasound extraction technology coupling with propyl-alcohol ammonium sulfate aqueous two-phase separation for extraction of polyphenols from mango nuclear was studied. The formation of aqueous two-phase and ultrasonic extraction conditions were optimized by orthogonal test,the optimum extraction conditions were; the propyl-alcohol solution (volume fraction as 60 % ),extraction time 15 min,0.30 ( NH_4)_2SO_4 0. 30 g/mL,the ratio of materials to solution as 1: 20 ( g/mL) . The extraction rates of polyphenols was 6. 96 % ( W/W),the purity of polyphenols was 37. 8 % (W/W) which was much higher than that of refluent ethanol extraction. The extractives singifi-cantly inhibit the lipid peroxidation (LPO) induced by ?OH free radical and hemoglobin ( RBC ) oxidate injury induced by free radical with the maximum inhibition rates of 66.8 % and 67.4 %,respectively. The proposed method is simply,mildly conditions and environmentally friend.

  17. Transport, geometrical, and topological properties of stealthy disordered hyperuniform two-phase systems.

    Science.gov (United States)

    Zhang, G; Stillinger, F H; Torquato, S

    2016-12-28

    Disordered hyperuniform many-particle systems have attracted considerable recent attention, since they behave like crystals in the manner in which they suppress large-scale density fluctuations, and yet also resemble statistically isotropic liquids and glasses with no Bragg peaks. One important class of such systems is the classical ground states of "stealthy potentials." The degree of order of such ground states depends on a tuning parameter χ. Previous studies have shown that these ground-state point configurations can be counterintuitively disordered, infinitely degenerate, and endowed with novel physical properties (e.g., negative thermal expansion behavior). In this paper, we focus on the disordered regime (0 two-phase media by circumscribing each point with a possibly overlapping sphere of a common radius a: the "particle" and "void" phases are taken to be the space interior and exterior to the spheres, respectively. The hyperuniformity of such two-phase media depends on the sphere sizes: While it was previously analytically proven that the resulting two-phase media maintain hyperuniformity if spheres do not overlap, here we show numerically that they lose hyperuniformity whenever the spheres overlap. We study certain transport properties of these systems, including the effective diffusion coefficient of point particles diffusing in the void phase as well as static and time-dependent characteristics associated with diffusion-controlled reactions. Besides these effective transport properties, we also investigate several related structural properties, including pore-size functions, quantizer error, an order metric, and percolation thresholds. We show that these transport, geometrical, and topological properties of our two-phase media derived from decorated stealthy ground states are distinctly different from those of equilibrium hard-sphere systems and spatially uncorrelated overlapping spheres. As the extent of short-range order increases, stealthy disordered

  18. Dynamic characteristics of two-phase thermal control system for spacecraft

    Science.gov (United States)

    Malozemov, Vladimir V.; Kudryavtseva, Natal'ya S.; Antonov, Viktor A.; Zagar, Oleg V.; Chernobaev, Nikolaj N.

    1992-07-01

    This paper deals with review of the issues associated with modelling the dynamic processes in the spacecraft two-phase thermal control systems. The work presents the results of modelling the nonstationary conditions of the evaporative and condensation heat exchangers functioning, investigates their response to the characteristic external influences. Disclosed are the results of the computer-aided modelling the two-phase thermal control system with a pump. The dynamic characteristics of the change in the inputs of pressures, temperatures and vapor content of a coolant in various branches of the system, as well as the lengths of the heat transfer zones in the evaporator and condenser under effect of the typical disturbing actions are obtained. The attained transients are analyzed.

  19. Efeitos do pH e massa molar do polímero sobre o sistema bifásico aquoso PEG/fosfato = Effects of polymer pH and molar mass on the PEG/phosphate aqueous two-phase system

    Directory of Open Access Journals (Sweden)

    Elias Basile Tambourgi

    2011-07-01

    Full Text Available A extração líquido-líquido usando sistemas bifásicos aquosos (SBA é uma técnica promissora na purificação de biomoléculas de interesse industrial e laboratorial. Este trabalho tem por objetivo apresentar um estudo do diagrama de equilíbrio de fases para diferentes massas molares de polietileno glicol (PEG (400 e 4000 e fosfato de potássio a pHs que variam entre 6 e 11, em condições normais de temperatura e pressão. A influência do peso molecular do PEG em diferentes pHs foram estudados nos diagramas de fases formados. Foram preparadas concentrações de 100 e 50% (p p-1 para o PEG 400 e 4000,respectivamente. A 20% (p p-1 de KH2PO4/K2HPO4, a solução estoque do sal fosfato foi também preparada de forma a obter os pHs desejados (6, 7, 8 e 9. Para os pHs 10 e 11 além dos sais, foi utilizada uma solução de NaOH 1M. Os resultados mostraram que menores concentrações de soluções foram utilizadas para formar o SBA com o PEG de maior massa molar. Por outro lado, não houve influência do pH nas curvas de equilíbrio, ou seja, um deslocamento significativo da binodal em relação aos diferentes pHs.Liquid-liquid extraction using aqueous two-phase systems (ATPSs is a promising technique for the purification of biomolecules of industrial and laboratory interest. This work presents a study of the phase diagrams for different poly(ethylene glycol (PEG molar masses (ranging from 400 to 4000 and potassium phosphate at pH varying from 6 to 11 in standard conditions. The influence of PEG molecular mass at different pHs was studied from the phase diagrams formed. Stocksolutions of PEG 400 and 4000 (100 and 50% w w-1, respectively and phosphate (20% w w-1 KH2PO4/K2HPO4 were prepared at the appropriate pH (6, 7, 8 and 9. For pHs 10 and 11, NaOH 1M was used in addition to the salts. The results showed that low concentrations were used to form the ATPS with high molecular mass PEG. On the other hand, there was no pH influence in the

  20. Separation of four flavonol glycosides from Solanum rostratum Dunal using aqueous two-phase flotation followed by preparative high-performance liquid chromatography.

    Science.gov (United States)

    Chang, Lin; Shao, Qian; Xi, Xingjun; Chu, Qiao; Wei, Yun

    2017-02-01

    Aqueous two-phase flotation followed by preparative high-performance liquid chromatography was used to separate four flavonol glycosides from Solanum rostratum Dunal. In the aqueous two-phase flotation section, the effects of sublation solvent, solution pH, (NH4 )2 SO4 concentration in aqueous solution, cosolvent, N2 flow rate, flotation time, and volumes of the polyethylene glycol phase on the recovery were investigated in detail, and the optimal conditions were selected: 50 wt% polyethylene glycol 1000 ethanol solvent as the flotation solvent, pH 4, 350 g/L of (NH4 )2 SO4 concentration in aqueous phase, 40 mL/min of N2 flow rate, 30 min of flotation time, 10.0 mL of flotation solvent volume, and two times. After aqueous two-phase flotation concentration, the flotation products were purified by preparative high-performance liquid chromatography. The purities of the final products A and B were 98.1 and 99.0%. Product B was the mixture of three compounds based on the analysis of high-performance liquid chromatography at the temperature of 10°C, while product A was hyperoside after the identification by nuclear magnetic resonance. Astragalin, 3'-O-methylquercetin 3-O-β-d-galactopyranoside, and 3'-O-methylquercetin 3-O-β-d-glucopyranoside were obtained with the purity of 93.8, 97.1, and 99.2%, respectively, after the further separation of product B using preparative high-performance liquid chromatography.

  1. Use of two-phase flow heat transfer method in spacecraft thermal system

    Science.gov (United States)

    Hye, A.

    1985-01-01

    In space applications, weight, volume and power are critical parameters. Presently liquid freon is used in the radiator planels of the Space Shuttle to dissipate heat. This requires a large amount of freon, large power for pumps, large volume and weight. Use of two-phase flow method to transfer heat can reduce them significantly. A modified commercial vapor compression refrigerator/freezer was sucessfully flown in STS-4 to study the effect of zero-gravity on the system. The duty cycle was about 5 percent higher in flight as compared to that on earth due to low flow velocity in condenser. The vapor Reynolds number at exit was about 4000 as compared to about 12,000. Efforts are underway to design a refrigerator/freezer using an oil-free compressor for Spacelab Mission 4 scheduled to fly in January 1986. A thermal system can be designed for spacecraft using the two-phase flow to transfer heat economically.

  2. Aqueous two phase system and ultrasonic wave coextraction of total flavonoids from Foeniculum vulgare stems and study on its antioxidation efficacy%超声波协同双水相体系提取茴香茎中总黄酮及其抗氧化性研究

    Institute of Scientific and Technical Information of China (English)

    贾贵华; 王金辉; 任雪峰

    2012-01-01

    Uhrasonic extraction technology integrated with ethanol and ammonium sulfate aqueous two-phase separation was used to extracl total flavnnoids from Foen,iculum vulgare stem. Based on single factor and orthogonal experiment, the optimum extracting conditions were as follows: 50% of ethanol, solid to liquid ratio of 1:15 and ultrasonic extracting for 20 rain. The order of factors to affect total flawmes extraction was: ethanol concentration〉ultrasonic time〉solid to liquid ratio. Determination showed thai ifs antioxidation efficacv was enhanced with the increasing of its concentration.%将超声波技术和双水相体系萃取技术综合应用于提取茴香茎中总黄酮。通过单因素试验和正交试验相结合,得出影响茴香茎中黄酮提取因素的主要次序为提取液浓度〉提取时间〉料液比。最佳提取工艺条件为乙醇体积分数50%、料液比1:15、超声时间20min。抗氧化能力检测表明,该提取物抗氧化性显著,且浓度越高,其抗氧化性越强。

  3. Two-phase distribution in the vertical flow line of a domestic wet central heating system

    Directory of Open Access Journals (Sweden)

    Ge Y.T.

    2013-04-01

    Full Text Available The theoretical and experimental aspects of bubble distribution in bubbly two-phase flow are reviewed in the context of the micro bubbles present in a domestic gas fired wet central heating system. The latter systems are mostly operated through the circulation of heated standard tap water through a closed loop circuit which often results in water supersaturated with dissolved air. This leads to micro bubble nucleation at the primary heat exchanger wall, followed by detachment along the flow. Consequently, a bubbly two-phase flow characterises the flow line of such systems. The two-phase distribution across the vertical and horizontal pipes was measured through a consideration of the volumetric void fraction, quantified through photographic techniques. The bubble distribution in the vertical pipe in down flow conditions was measured to be quasi homogenous across the pipe section with a negligible reduction in the void fraction at close proximity to the pipe wall. Such a reduction was more evident at lower bulk fluid velocities.

  4. Two-phase distribution in the vertical flow line of a domestic wet central heating system

    Science.gov (United States)

    Fsadni, A.-M.; Ge, Y. T.

    2013-04-01

    The theoretical and experimental aspects of bubble distribution in bubbly two-phase flow are reviewed in the context of the micro bubbles present in a domestic gas fired wet central heating system. The latter systems are mostly operated through the circulation of heated standard tap water through a closed loop circuit which often results in water supersaturated with dissolved air. This leads to micro bubble nucleation at the primary heat exchanger wall, followed by detachment along the flow. Consequently, a bubbly two-phase flow characterises the flow line of such systems. The two-phase distribution across the vertical and horizontal pipes was measured through a consideration of the volumetric void fraction, quantified through photographic techniques. The bubble distribution in the vertical pipe in down flow conditions was measured to be quasi homogenous across the pipe section with a negligible reduction in the void fraction at close proximity to the pipe wall. Such a reduction was more evident at lower bulk fluid velocities.

  5. Influence of ultrasonic condition on phase transfer catalyzed radical polymerization of methyl methacrylate in two phase system - A kinetic study.

    Science.gov (United States)

    Marimuthu, Elumalai; Murugesan, Vajjiravel

    2016-08-25

    An ultrasonic condition assisted phase transfer catalyzed radical polymerization of methyl methacrylate was investigated in an ethyl acetate/water two phase system at 60±1°C and 25kHz, 300W under inert atmosphere. The influence of monomer, initiator, catalyst and temperature, volume fraction of aqueous phase on the rate of polymerization was examined in detail. The reaction order was found to be unity for monomer, initiator and catalyst. Generally, the reaction rate was relatively fast in two phase system, when a catalytic amount of phase transfer catalyst was used. The combined approach, use of ultrasonic and PTC condition was significantly enhances the rate of polymerization. An ultrasonic and phase transfer catalyzed radical polymerization of methyl methacrylate has shown about three fold enhancements in the rate compared with silent polymerization of MMA using cetyltrimethylammonium bromide as PTC. The resultant kinetics was evaluated with silent polymerization and an important feature was discussed. The activation energy and other thermodynamic parameters were computed. Based on the obtained results an appropriate radical mechanism has been derived. TGA showed the polymer was stable up to 150°C. The FT-IR and DSC analysis validates the atactic nature of the obtained polymer. The XRD pattern reveals the amorphous nature of polymer was dominated.

  6. A novel anaerobic two-phase system for biohydrogen production and in situ extraction of organic acid byproducts.

    Science.gov (United States)

    Sarma, Saurabh Jyoti; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo

    2015-06-01

    Owing to CO2-free emission, hydrogen is considered as a potential green alternative of fossil fuels. Water is the major emission of hydrogen combustion process and gravimetric energy density of hydrogen is nearly three times more than that of gasoline and diesel fuel. Biological hydrogen production, therefore, has commercial significance; especially, when it is produced from low-cost industrial waste-based feedstock. Light independent anaerobic fermentation is simple and mostly studied method of biohydrogen production. During hydrogen production by this method, a range of organic acid byproducts are produced. Accumulation of these byproducts is inhibitory for hydrogen production as it may result in process termination due to sharp decrease in medium pH or by possible metabolic shift. For the first time, therefore, a two-phase anaerobic bioreactor system has been reported for biohydrogen production which involves in situ extraction of different organic acids. Among different solvents, based on biocompatibility oleyl alcohol has been chosen as the organic phase of the two-phase system. An organic:aqueous phase ratio of 1:50 has been found to be optimum for hydrogen production. The strategy was capable of increasing the hydrogen production from 1.48 to 11.65 mmol/L-medium.

  7. Economic analysis of uricase production under uncertainty: Contrast of chromatographic purification and aqueous two-phase extraction (with and without PEG recycle).

    Science.gov (United States)

    Torres-Acosta, Mario A; Aguilar-Yáñez, José M; Rito-Palomares, Marco; Titchener-Hooker, Nigel J

    2016-01-01

    Uricase is the enzyme responsible for the breakdown of uric acid, the key molecule leading to gout in humans, into allantoin, but it is absent in humans. It has been produced as a PEGylated pharmaceutical where the purification is performed through three sequential chromatographic columns. More recently an aqueous two-phase system (ATPS) was reported that could recover Uricase with high yield and purity. Although the use of ATPS can decrease cost and time, it also generates a large amount of waste. The ability, therefore, to recycle key components of ATPS is of interest. Economic modelling is a powerful tool that allows the bioprocess engineer to compare possible outcomes and find areas where further research or optimization might be required without recourse to extensive experiments and time. This research provides an economic analysis using the commercial software BioSolve of the strategies for Uricase production: chromatographic and ATPS, and includes a third bioprocess that uses material recycling. The key parameters that affect the process the most were located via a sensitivity analysis and evaluated with a Monte Carlo analysis. Results show that ATPS is far less expensive than chromatography, but that there is an area where the cost of production of both bioprocesses overlap. Furthermore, recycling does not impact the cost of production. This study serves to provide a framework for the economic analysis of Uricase production using alternative techniques.

  8. Integrated method of thermosensitive triblock copolymer-salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide.

    Science.gov (United States)

    Wang, Yun; Hu, Xiaowei; Han, Juan; Ni, Liang; Tang, Xu; Hu, Yutao; Chen, Tong

    2016-03-01

    A polymer-salt aqueous two-phase system (ATPS) consisting of thermosensitive copolymer ethylene-oxide-b-propylene-oxide-b-ethylene-oxide (EOPOEO) and NaH2PO4 was employed in deproteinization for lycium barbarum polysaccharide (LBP). The effects of salt type and concentration, EOPOEO concentration, amount of crude LBP solution and temperature were studied. In the primary extraction process, LBP was preferentially partitioned to the bottom (salt-rich) phase with high recovery ratio of 96.3%, while 94.4% of impurity protein was removed to the top (EOPOEO-rich) phase. Moreover, the majority of pigments could be discarded to top phase. After phase-separation, the LBP in the bottom phase was further purified by dialysis membrane to remove salt and other small molecular impurities. The purity of LBP was enhanced to 64%. Additionally, the FT-IR spectrum was used to identify LBP. EOPOEO was recovered by a temperature-induced separation, and reused in a new ATPS. An ideal extraction and recycle result were achieved.

  9. Two-Phase Cryogenic Heat Exchanger for the Thermodynamic Vent System

    Science.gov (United States)

    Christie, Robert J.

    2011-01-01

    A two-phase cryogenic heat exchanger for a thermodynamic vent system was designed and analyzed, and the predicted performance was compared with test results. A method for determining the required size of the Joule-Thomson device was also developed. Numerous sensitivity studies were performed to show that the design was robust and possessed a comfortable capacity margin. The comparison with the test results showed very similar heat extraction performance for similar inlet conditions. It was also shown that estimates for Joule- Thomson device flow rates and exit quality can vary significantly and these need to be accommodated for with a robust system design.

  10. Fractionation of wheat gliadins by counter-current distribution using an organic two-phase system.

    Science.gov (United States)

    Truust, H; Johansson, G

    1998-06-26

    A liquid liquid two-phase system based on N,N-dimethylformamide and the two polymers, poly(ethyleneglycol) and Ficoll, useful for partitioning of hydrophobic proteins, has been developed. The system has been applied to a counter-current distribution process in 56 steps for analysing the heterogeneity of proteins extracted with N,N-dimethylformamide from wheat flour. The counter-current distribution patterns of proteins, extracted from eight kinds of wheat, have been analysed. The minimum number of hypothetical proteins necessary to describe the patterns was found to be seven. The relative amount of these hypothetical components varied among the wheats.

  11. 正交提取双水相体系纯化新楝果核印楝素的工艺研究%Study on separation of azadirachtin from new neem kernels by orthogonal design mode and aqueous two-phase system

    Institute of Scientific and Technical Information of China (English)

    王曼; 王佳静; 栾龙; 孙润广

    2014-01-01

    采用乙醇-无机盐双水相技术,研究了从国产新楝果核中提取印楝素的工艺.通过单因素实验和正交实验探讨了无机盐的质量浓度、提取时间、料液比对印楝素提取率的影响.实验结果表明:正交提取、双水相体系纯化印楝素的最佳条件是:无机盐浓度为0.62 g/mL,提取时间为1.5 h,料液比为1∶10(g∶mL),此时印楝素的提取率为1.23%.%The separation of azadirachtin from new neem kernels was studied by alcohol-inorganic salt a-queous two-phase extraction technology.The single factor and orthogonal experiments were done to in-vestigate the varitaion of azadirachtin extraction ratio relating to inorganic salt concentration,extraction time and material-solvent ratio.The results showed that the extraction rate of azadirachtin was 1 .2 3%under the optimum condition that the concentration of inorganic salt was 0.62 g/mL,the extraction time was 1 .5 h,and the material-solvent ratio was 1∶1 0 (g∶mL).

  12. Enhanced translocation and growth of Rhodococcus erythropolis PR4 in the alkane phase of aqueous-alkane two phase cultures were mediated by GroEL2 overexpression.

    Science.gov (United States)

    Takihara, Hayato; Ogihara, Jun; Yoshida, Takao; Okuda, Shujiro; Nakajima, Mutsuyasu; Iwabuchi, Noriyuki; Sunairi, Michio

    2014-01-01

    We previously reported that R. erythropolis PR4 translocated from the aqueous to the alkane phase, and then grew in two phase cultures to which long-chain alkanes had been added. This was considered to be beneficial for bioremediation. In the present study, we investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane (C19) phase than in those located at the aqueous-alkane interface attached to the n-dodecane (C12) surface. PR4 (pK4-EL2-1) and PR4 (pK4-ΔEL2-1) strains were constructed to confirm the effects of the upregulation of GroEL2 in translocated cells. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4-ΔEL2-1) in two phase cultures containing C12. The growth and cell surface lipophilicity of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the plasmid overexpression of groEL2 in PR4 (pK4-EL2-1) led to changes in cell localization, enhanced growth, and increased cell surface lipophilicity. Thus, we concluded that the overexpression of GroEL2 may play an important role in increasing the organic solvent tolerance of R. erythropolis PR4 in aqueous-alkane two phase cultures.

  13. A comparative study of solid and liquid non-aqueous phases for the biodegradation of hexane in two-phase partitioning bioreactors.

    Science.gov (United States)

    Hernández, María; Quijano, Guillermo; Thalasso, Frédéric; Daugulis, Andrew J; Villaverde, Santiago; Muñoz, Raúl

    2010-08-01

    A comparative study of the performance of solid and liquid non-aqueous phases (NAPs) to enhance the mass transfer and biodegradation of hexane by Pseudomonas aeruginosa in two-phase partitioning bioreactors (TPPBs) was undertaken. A preliminary NAP screening was thus carried out among the most common solid and liquid NAPs used in pollutant biodegradation. The polymer Kraton G1657 (solid) and the liquid silicone oils SO20 and SO200 were selected from this screening based on their biocompatibility, resistance to microbial attack, non-volatility and high affinity for hexane (low partition coefficient: K = C(g)/C(NAP), where C(g) and C(NAP) represent the pollutant concentration in the gas phase and NAP, respectively). Despite the three NAPs exhibited a similar affinity for hexane (K approximately 0.0058), SO200 and SO20 showed a superior performance to Kraton G1657 in terms of hexane mass transfer and biodegradation enhancement. The enhanced performance of SO200 and SO20 could be explained by both the low interfacial area of this solid polymer (as a result of the large size of commercial beads) and by the interference of water on hexane transfer (observed in this work). When Kraton G1657 (20%) was tested in a TPPB inoculated with P. aeruginosa, steady state elimination capacities (ECs) of 5.6 +/- 0.6 g m(-3) h(-1) were achieved. These values were similar to those obtained in the absence of a NAP but lower compared to the ECs recorded in the presence of 20% of SO200 (10.6 +/- 0.9 g m(-3) h(-1)). Finally, this study showed that the enhancement in the transfer of hexane supported by SO200 was attenuated by limitations in microbial activity, as shown by the fact that the ECs in biotic systems were far lower than the maximum hexane transfer capacity recorded under abiotic conditions.

  14. Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC.

    Science.gov (United States)

    Chen, Zhi; Zhang, Wei; Tang, Xunyou; Fan, Huajun; Xie, Xiujuan; Wan, Qiang; Wu, Xuehao; Tang, James Z

    2016-06-25

    A novel and rapid method for simultaneous extraction and separation of the different polysaccharides from Semen Cassiae (SC) was developed by microwave-assisted aqueous two-phase extraction (MAATPE) in a one-step procedure. Using ethanol/ammonium sulfate system as a multiphase solvent, the effects of MAATPE on the extraction of polysaccharides from SC such as the composition of the ATPS, extraction time, temperature and solvent-to-material ratio were investigated by UV-vis analysis. Under the optimum conditions, the yields of polysaccharides were 4.49% for the top phase, 8.80% for the bottom phase and 13.29% for total polysaccharides, respectively. Compared with heating solvent extraction and ultrasonic assisted extraction, MAATPE exhibited the higher extraction yields in shorter time. Fourier-transform infrared spectra showed that two polysaccharides extracted from SC to the top and bottom phases by MAATPE were different from each other in their chemical structures. Through acid hydrolysis and PMP derivatization prior to HPLC, analytical results by indicated that a polysaccharide of the top phases was a relatively homogeneous homepolysaccharide composed of dominant gucose glucose while that of the bottom phase was a water-soluble heteropolysaccharide with multiple components of glucose, xylose, arabinose, galactose, mannose and glucuronic acid. Molar ratios of monosaccharides were 95.13:4.27:0.60 of glucose: arabinose: galactose for the polysaccharide from the top phase and 62.96:14.07:6.67: 6.67:5.19:4.44 of glucose: xylose: arabinose: galactose: mannose: glucuronic acid for that from the bottom phase, respectively. The mechanism for MAATPE process was also discussed in detail. MAATPE with the aid of microwave and the selectivity of the ATPS not only improved yields of the extraction, but also obtained a variety of polysaccharides. Hence, it was proved as a green, efficient and promising alternative to simultaneous extraction of polysaccharides from SC.

  15. Silver nanoplates with ground or metastable structures obtained from template-free two-phase aqueous/organic synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Zhelev, Doncho V., E-mail: dontcho.jelev@nih.gov; Zheleva, Tsvetanka S. [Army Research Laboratory, 2800 Adelphi, Maryland 20783 (United States)

    2014-01-28

    Silver has unique electrical, catalytic, and plasmonic characteristics and has been widely sought for fabrication of nanostructures. The properties of silver nanostructures are intimately coupled to the structure of silver crystals. Two crystal structures are known for silver: the stable (ground) state cubic face centered 3C-Ag structure and the metastable hexagonal 4H-Ag structure. Recently, Chackraborty et al. [J. Phys.: Condens. Matter 23, 325401 (2011)] discovered a low density, highly reactive metastable hexagonal 2H-Ag structure accessible during electrodeposition of silver nanowires in porous anodic alumina templates. This 2H-Ag structure has enhanced electrical and catalytic characteristics. In the present work we report template-free synthesis of silver nanoplates with the metastable 2H-Ag crystal structure, which appears together with the ground 3C-Ag and the metastable 4H-Ag structures in a two-phase solution synthesis with citric acid as the capping agent. The capacity of citric acid to stabilize both the stable and the metastable structures is explained by its preferential binding to the close packed facets of Ag crystals, which are the (111) planes for 3C-Ag and the (0001) planes for 4H-Ag and 2H-Ag. Nanoplate morphology and structure are characterized using scanning electron microscopy, X-ray diffraction, and transmission electron microscopy. The synthesized nanoplates have thickness from 15 to 17 nm and edge length from 1 to 10 μm. Transmission electron microscopy selected area electron diffraction is used to uniquely identify and distinguish between nanoplates with 2H-Ag or 4H-Ag or 3C-Ag structures.

  16. Ionic liquid and aqueous two-phase extraction based on salting-out coupled with high-performance liquid chromatography for the determination of seven rare ginsenosides in Xue-Sai-Tong injection.

    Science.gov (United States)

    Li, Lan-Jie; Jin, Yong-Ri; Wang, Xiao-Zhong; Liu, Ying; Wu, Qian; Shi, Xiao-Lei; Li, Xu-Wen

    2015-09-01

    A method of ionic liquid salt aqueous two-phase extraction coupled with high-performance liquid chromatography has been developed for the analysis of seven rare ginsenosides including Rg6 , F4 , 20(S)-Rg3 , 20(R)-Rg3 , Rk3 , Rk1 , and Rg5 in Xue-Sai-Tong injection. The injection was mixed with ionic liquid 1-butyl-3-methylimidazolium bromide aqueous solution, and a mixture was obtained. With the addition of sodium dodecyl sulfate and dipotassium phosphate into the mixture, the aqueous two-phase mixture was formed after ultrasonic treatment and centrifuged. Rare ginsenosides were extracted into the upper phase. To obtain a high extraction factors, various influences were considered systematically, such as the volume of ionic liquid, the category and amount of salts, the amount of sodium dodecyl sulfate, the pH value of system, and the time of ultrasonic treatment. Under the optimal condition, rare ginsenosides in Xue-Sai-Tong injection were enriched and detected, the recoveries of seven rare ginsenosides ranged from 90.05 to 112.55%, while relative standard deviations were lower than 2.50%. The developed method was reliable, rapid and sensitive for the determination of seven rare ginsenosides in the injections.

  17. Optimizing steam flood performance utilizing a new and highly accurate two phase steam measurement system

    Energy Technology Data Exchange (ETDEWEB)

    Huff, B. D.; Warren, P. B. [CalResources LLC (Canada); Whorff, F. [ITT Barton (Canada)

    1995-11-01

    The development of a two phase steam measurement system was documented. The system consists of a `V` cone differential pressure device and a vortex meter velocity device in series through which the steam flows. Temperature and pressure sensors are electronically interfaced with a data logging system. The design was described as being very simple and rugged, consequently, well suited to monitoring in the field.. Steam quality measurements were made in the Kern River Field and the Coalinga Field thermal projects using a surface steam separator. In steam flood operations, steam cost is very high, hence appropriate distribution of the steam can result in significant cost reduction. This technology allows the measurement of steam flow and quality at any point in the steam distribution system. The metering system`s orifice meter was found to have a total average error of 45%, with 25% of that attributable to `cold leg` problem. Installation of the metering system was expected to result in a steam use reduction of 8%, without any impact on production. Steam re-distribution could result in a potential oil production increase of 10%. 12 refs., 8 tabs., 9 figs.

  18. Reaction Mechanisms and Particle Interaction in Burning Two-Phase Systems

    Science.gov (United States)

    Dreizin, Edward L.; Shoshin, Yuriy L.; Murdyy, Ruslan S.; Hoffmann, Vern K.

    2001-01-01

    The main objective of this research is to understand the mechanisms by which particle interactions affect ignition and combustion in the two-phase systems. Combustion of metal aerosols representing the two-phase systems is carried out in the microgravity environment enabling one to avoid the buoyant flows that mask the particle motion due to the particle-particle interaction effects. In addition, relatively large, e.g., 100 micron diameter particles can be used, that remain aerosolized (i.e., do not fall down as they would at normal gravity) so that their behavior ahead, behind, and within the propagating flame can be resolved optically. An experimental apparatus exploiting this approach has been designed for the 2.2-s drop tower microgravity experiments. A typical experiment includes fluidizing metal particles under microgravity in an acoustic field, turning off the acoustic exciter, and igniting the created aerosol at a constant pressure using a hot wire igniter. The flame propagation and details of the individual particle combustion and particle interactions are studied using high-speed movie and video cameras coupled with microscope lenses to resolve individual particles. Recorded flame images are digitized and various image processing techniques including flame position tracking, color separation, and pixel by pixel image comparisons are employed to understand the processes occurring in the burning aerosols. Condensed combustion products are collected after each experiment for the phase, composition, and morphology analyses. New experiments described in this paper address combustion of Ti and Al particle clouds in air and combustion of Mg particle clouds in CO2. In addition, microgravity combustion experiments have been conducted with the particles of the newly produced Al-Mg mechanical alloys aerosolized in air.

  19. Space qualification of an experimental two-phase flow thermal management system

    Science.gov (United States)

    Koonmen, James P.; Carswell, Lisa C.; Kvansnak, Michael A.

    1991-01-01

    The Weapons Laboratory will launch a space experiment in March 1991 to investigate the effects of extended microgravity on two-phase (liquid/vapor) flow. The qualification process for the experimental flight system hardware differs significantly from the process used for complex, high cost, long life space systems. Some development, qualification, and acceptance tests normally included in the test program of an operational space system were omitted because of the low program cost and low consequence of experiment failure. Key environment and functional qualification tests were performed, however, in an effort to reduce the risk of failure inherent in any space mission. The environmental qualification program included short duration vacuum chamber tests, reduced gravity missions onboard a National Aeronautics and Space Administration (NASA) test aircraft, and a complete series of shock and vibration tests. The functional qualification program centered on thermal-hydraulic system performance tests and a complete check-out of the unique telemetry system used to retrieve the experimental data from the payload. The test program also contains a number of acceptance and prelaunch validation tests to be performed as final verification of payloads readiness for spaceflight.

  20. Two-phase equilibrium and molecular hydrogen formation in damped Lyman-alpha systems

    CERN Document Server

    Liszt, H S

    2002-01-01

    Molecular hydrogen is quite underabundant in damped Lyman-alpha systems at high redshift, when compared to the interstellar medium near the Sun. This has been interpreted as implying that the gas in damped Lyman-alpha systems is warm. like the nearby neutral intercloud medium, rather than cool, as in the clouds which give rise to most H I absorption in the Milky Way. Other lines of evidence suggest that the gas in damped Lyman-alpha systems -- in whole or part -- is actually cool; spectroscopy of neutral and ionized carbon, discussed here, shows that the damped Lyman-alpha systems observed at lower redshift z $$ 2.8 are warm (though not devoid of H2). To interpret the observations of carbon and hydrogen we constructed detailed numerical models of H2 formation under the conditions of two-phase thermal equilibrium, like those which account for conditions near the Sun, but with varying metallicity, dust-gas ratio, $etc$. We find that the low metallicity of damped Lyman-alpha systems is enough to suppress H2 form...

  1. Chemiluminescence detection of organic peroxides in a two-phase system.

    Science.gov (United States)

    Baj, Stefan; Krawczyk, Tomasz

    2007-02-28

    Potential application of chemiluminescence (CL) reaction of luminol for detection of organic peroxides by HPLC post-column reaction using an immiscible apolar eluent (hexane) with aqueous solution of luminol has been proposed. The positive influence of the addition of an anionic surface-active agent--sodium dodecyl sulphate (SDS) to the luminol solution on the CL intensity has been observed. The sensitivity of the method is greater for peroxyacid and hydroperoxide tested, and lesser for diacyl peroxide as compared to system with polar eluent miscible with solution of luminol. Our interpretation of observed results based on the extraction efficiency, CL kinetics and microemulsion formation has been suggested.

  2. Contribution to the thermodynamics of sc3+, y3+, la3+ and trivalent lanthanide cations in the two-phase water-nitrobenzene extraction system.

    Science.gov (United States)

    Makrlík, Emanuel; Vaňura, Petr

    2011-06-01

    From the exchange extraction constants corresponding to the general equilibrium M3+(aq) + 3H+(nb) M3+(nb) + 3H+(aq) occurring in the two-phase water-nitrobenzene system (M3+ = Sc3+, Y3+, La3+, Ce3+, Pr3+, Nd3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+, Yb3+, Lu3+; aq = aqueous phase, nb = nitrobenzene phase), the individual extraction constants of 16 trivalent metal cations were calculated. It was found that these individual extraction constants in the mentioned two-phase system increase in the following cation order: Sc3+ < Yb3+< Ho3+ < Er3+ < Tm3+, Lu3+ < Y3+, Dy3+ < Tb3+ < Gd3+ < Eu3+ < Sm3+ < Nd3+ < Pr3+ < La3+, Ce3+.

  3. Aqueous two-phase based on ionic liquid liquid-liquid microextraction for simultaneous determination of five synthetic food colourants in different food samples by high-performance liquid chromatography.

    Science.gov (United States)

    Sha, Ou; Zhu, Xiashi; Feng, Yanli; Ma, Weixing

    2015-05-01

    A rapid and effective method of aqueous two-phase systems based on ionic liquid microextraction for the simultaneous determination of five synthetic food colourants (tartrazine, sunset yellow, amaranth, ponceau 4R and brilliant blue) in food samples was established. High-performance liquid chromatography coupled with an ultraviolet detector of variable wavelength was used for the determinations. 1-alkyl-3-methylimidazolium bromide was selected as the extraction reagent. The extraction efficiency of the five colourants in the proposed system is influenced by the types of salts, concentrations of salt and [CnMIM]Br, as well as the extracting time. Under the optimal conditions, the extraction efficiencies for these five colourants were above 95%. The phase behaviours of aqueous two-phase system and extraction mechanism were investigated by UV-vis spectroscopy. This method was applied to the analysis of the five colourants in real food samples with the detection limit of 0.051-0.074 ng/mL. Good spiked recoveries from 93.2% to 98.9% were obtained.

  4. Effective thermal conductivity of real two-phase systems using resistor model with ellipsoidal inclusions

    Indian Academy of Sciences (India)

    Jagjiwanram; Ramvir Singh

    2004-08-01

    A theoretical model has been developed for real two-phase system assuming linear flow of heat flux lines having ellipsoidal particles arranged in a three-dimensional cubic array. The arrangement has been divided into unit cells, each of which contains an ellipsoid. The resistor model has been applied to determine the effective thermal conductivity (ETC) of the unit cell. To take account of random packing of the phases, non-uniform shape of the particles and non-linear flow of heat flux lines in real systems, incorporating an empirical correction factor in place of physical porosity modifies an expression for ETC. An effort is made to correlate it in terms of the ratio of thermal conductivities of the constituents and the physical porosity. Theoretical expression so obtained has been tested on a large number of samples cited in the literature and found that the values predicted are quite close to the experimental results. Comparison of our model with different models cited in the literature has also been made.

  5. Separation Behavior of Penicillin in Aqueous Two-Phase Flotation%双水相浮选过程中青霉素的分离行为

    Institute of Scientific and Technical Information of China (English)

    毕鹏禹; 常林; 董慧茹

    2011-01-01

    基于双水相浮选技术(ATPF)分离富集水相中青霉素的方法,研究了双水相浮选过程中青霉素的分离行为.在常温下,2.5 g/L青霉素水溶液300 mL、初始pH 7、(NH4)2SO4浓度350 g/L、浮选溶剂为50%(w/w)PEG1000水溶液10 mL条件下,分别研究了青霉素在双水相浮选过程中的动力学行为和分离后的赋存状态.3种通气流速条件下的动力学研究结果表明,青霉素分离过程存在两个阶段,分别是沉淀浮选阶段和分子吸附浮选阶段,两个阶段均遵守一级动力学;双水相浮选中有机相的红外差谱表明,青霉素以离子形态直接溶解于聚乙二醇相,这是双水相浮选分离效率明显高于传统萃取技术的根本原因.%Based on the application of aqueous two-phase flotaion (ATPF) in the separation and concentration of penicillin from aqueous phase, the separation behavior of penicillin in the ATPF process was investigated. The kinetic behavior and the penicillin status in organic phase were studied under the conditions of room temperature, 300 mL penicillin aqueous solution of 2.5 g/L, initial pH 7.0, ammonium sulfate concentration of 350 g/L, 10 mL polyethyleneglycol (PEG) aqueous solution (50%, w/w ). Using three flow rates, the kinetic results show that there are two stages in the separation process of ATPF: (1) precipitation flotation and (2) molecular adsorption flotation, and both of them follow the fist order kinetics equation. Moreover, the subtractive spectroscopy of organic phase shows that penicillin ion can directly dissolve into PEG phase. This is the reason that the separation efficiency of ATPF is significantly higher than that of the traditional extraction techniques.

  6. Releasing intracellular product to prepare whole cell biocatalyst for biosynthesis of Monascus pigments in water-edible oil two-phase system.

    Science.gov (United States)

    Hu, Minglue; Zhang, Xuehong; Wang, Zhilong

    2016-11-01

    Selective releasing intracellular product in Triton X-100 micelle aqueous solution to prepare whole cell biocatalyst is a novel strategy for biosynthesis of Monascus pigments, in which cell suspension culture exhibits some advantages comparing with the corresponding growing cell submerged culture. In the present work, the nonionic surfactant Triton X-100 was successfully replaced by edible plant oils for releasing intracellular Monascus pigments. High concentration of Monascus pigments (with absorbance nearly 710 AU at 470 nm in the oil phase, normalized to the aqueous phase volume approximately 142 AU) was achieved by cell suspension culture in peanut oil-water two-phase system. Furthermore, the utilization of edible oil as extractant also fulfills the demand for application of Monascus pigments as natural food colorant.

  7. 丙烯酰胺/丙烯酸/甲基丙烯酸甲酯双水相共聚过程的成滴机理%Droplet formation mechanism of acrylamide/acrylic acid/methyl methacrylate aqueous two-phase copolymerization

    Institute of Scientific and Technical Information of China (English)

    蔡晓生; 单国荣

    2012-01-01

    Acrylamide (AM)/acrylic acid (AA )/methyl methacrylate (MMA) copolymer with the stabilizer poly (acrylic acid sodium) (PAANa) dispersed in the ammonium sulfate (AS) solution was prepared through aqueous two-phase copolymerization. By using dynamic light scattering (DLS), the droplet appearance and growth in the initial stage of the aqueous two-phase copolymerization was on-line detected, and the results showed that the copolymer droplet aggregated with each other at the initial stage. Therefore, the droplet size distribution became wide at first, and then turned to narrow again as the copolymerization proceeded. By using gas chromatography, the exact monomer residues were detected and the composition of the copolymer chain was calculated. The reaction could be divided into three stages. The AM and MMA segments were the main part of the copolymer chain at the first stage, at the second stage AM grew mainly into the copolymer chain, and the A A and AM segments were the main part of the copolymer chain at the third stage. Eventually, the droplet formation mechanism of the aqueous two-phase copolymerization of AM/AA/MMA with the stabilizer PAANa in AS aqueous solution was presented. The negative charge of the AA segment in the copolymer chain was considered as a significant factor in the stabilization of the aqueous two-phase system.

  8. Two-phase experimental heat transfer studies on a water-diesel system in a shell and tube heat exchanger

    Directory of Open Access Journals (Sweden)

    V. Alagesan

    2012-06-01

    Full Text Available Two-phase heat transfer involving two immiscible systems is gaining importance in petrochemical and allied industries. Varying compositions of diesel and water were experimentally studied in a 1:2 shell and tube heat exchanger. The data on pure water and diesel were fitted to an equation of the form. h1φ = a NmRe.The two-phase multiplier, Φ L, was related to the Lockhart Martinelli (L-M parameter, χtt², using the two-phase data and a correlation Φ L = b+c(χtt²+d/(χtt²² was established. The two-phase heat transfer coefficient was calculated based on the coefficients 'a' and 'm' for pure diesel and pure water along with ФL and the L-M parameter. The calculated values of the two-phase heat transfer coefficient h2φ based on pure diesel and pure water suggest that diesel is a better reference fluid since the average error is much smaller compared to pure water as reference.

  9. A numerical method for a model of two-phase flow in a coupled free flow and porous media system

    KAUST Repository

    Chen, Jie

    2014-07-01

    In this article, we study two-phase fluid flow in coupled free flow and porous media regions. The model consists of coupled Cahn-Hilliard and Navier-Stokes equations in the free fluid region and the two-phase Darcy law in the porous medium region. We propose a Robin-Robin domain decomposition method for the coupled Navier-Stokes and Darcy system with the generalized Beavers-Joseph-Saffman condition on the interface between the free flow and the porous media regions. Numerical examples are presented to illustrate the effectiveness of this method. © 2014 Elsevier Inc.

  10. A simple method to optimize the HSCCC two-phase solvent system by predicting the partition coefficient for target compound.

    Science.gov (United States)

    Han, Quan-Bin; Wong, Lina; Yang, Nian-Yun; Song, Jing-Zheng; Qiao, Chun-Feng; Yiu, Hillary; Ito, Yoichiro; Xu, Hong-Xi

    2008-04-01

    A simple method was developed to optimize the solvent ratio of the two-phase solvent system used in the high-speed counter-current chromatography (HSCCC) separation. Some mathematic equations, such as the exponential and the power equations, were established to describe the relationship between the solvent ratio and the partition coefficient. Using this new method, the two-phase solvent system was easily optimized to obtain a proper partition coefficient for the CCC separation of the target compound. Furthermore, this method was satisfactorily applied in determining the two-phase solvent system for the HSCCC preparation of pseudolaric acid B from the Chinese herb Pseudolarix kaempferi Gordon (Pinaceae). The two-phase solvent system of n-hexane/EtOAc/MeOH/H(2)O (5:5:5:5 by volume) was used with a good partition coefficient K = 1.08. As a result, 232.05 mg of pseudolaric acid B was yielded from 0.5 g of the crude extract with a purity of 97.26% by HPLC analysis.

  11. Two-phase plate-fin heat exchanger modeling for waste heat recovery systems in diesel engines

    NARCIS (Netherlands)

    Feru, E.; de Jager, B.; Willems, F.; Steinbuch, M.

    2014-01-01

    This paper presents the modeling and model validation for a modular two-phase heat exchanger that recovers energy in heavy-duty diesel engines. The model is developed for temperature and vapor quality prediction and for control design of the waste heat recovery system. In the studied waste heat reco

  12. Study of nonionic surfactant aqueous two-phase extraction of amino acids%非离子表面活性剂双水相萃取氨基酸研究

    Institute of Scientific and Technical Information of China (English)

    孙晨; 刘广宇; 徐培辉

    2014-01-01

    This paper mainly researches on the phase behavior of the extraction distribution amino acid extract in nonionic surfactant in aqueous two-phase system. Extraction of L-phenylalanine with AEO-7/Na3PO4 aqueous two-phase,the effects of the content of AEO-7 and salt(NaCl,Na2SO4, Na3PO4),adding L-phenylalanine content of extracts,extraction time,extraction temperature were investigated. The results showed that the optimal conditions of AEO-7/Na3PO4,aqueous two-phase extraction of L-phenylalanine is volume concentration when AEO-7 was 8%,concentration of Na3PO4·12H2O for 85 g/L,temperature 40℃,concentration of L-phenylalanine is 0.532 8 g/L,time 60 min extraction of L-phenylalanine rate can reach 98.7%,the distribution coefficient of 15.3.%该文对氨基酸萃取液在非离子表面活性剂双水相体系中的萃取分配相行为进行研究。采用脂肪醇聚氧乙烯醚AEO-7/盐双水相萃取L-苯丙氨酸,考察了加盐(NaCl、Na2SO4、Na3PO4)、AEO-7含量、萃取时间、加入萃取物L-苯丙氨酸的含量以及萃取温度对双水相及萃取分离L-苯丙氨酸的影响。结果表明,AEO-7/Na3PO4双水相萃取L-苯丙氨酸的适宜条件是当AEO-7的体积浓度为8%,Na3PO4·12H2O的质量浓度为85 g/L,温度为40℃,L-苯丙氨酸的质量浓度为0.5328 g/L,时间为60 min时L-苯丙氨酸的萃取率可以达到98.7%,分配系数为15.3。

  13. An Isotopic Dilution Experiment Using Liquid Scintillation: A Simple Two-System, Two-Phase Analysis.

    Science.gov (United States)

    Moehs, Peter J.; Levine, Samuel

    1982-01-01

    A simple isotonic, dilution analysis whose principles apply to methods of more complex radioanalyses is described. Suitable for clinical and instrumental analysis chemistry students, experimental manipulations are kept to a minimum involving only aqueous extraction before counting. Background information, procedures, and results are discussed.…

  14. Interfacial thermodynamics and electrochemistry of protein partitioning in two-phase systems.

    NARCIS (Netherlands)

    Fraaije, J.G.E.M.

    1987-01-01

    The subject of this thesis is protein partition between an aqueous salt solution and a surface or an apolair liquid and the concomitant co-partition of small ions. The extent of co-partitioning determines the charge regulation in the protein partitioning process.Chapters 2 and 3 deal with phenomenol

  15. Two-phase Flow of Liquid-gas in Diesel Fuel Injection System and Their Effect on Engine Performances

    Institute of Scientific and Technical Information of China (English)

    Yongling He; Zhihe Zhao; Jianxin Liu; Huiyong Du; Min Li; Yongping Zong

    2001-01-01

    In this paper, by using high-speed camera, CCD camera, signal and graph acquisition system, and other experimental instruments, investigation on liquid-gas two-phase flow in diesel fuel injection system and their effect on engine performances were made. Emerging and bursting of cavitation in the cavity above pump delivery valve, in injection pipe, and in fuel trough of injector of the fuel injection system were observed and mechanism of cavitation were discussed. Effects of liquid-gas two-phase flow on propagation velocity of pressure wave of the system and on irregular injection were analyzed. Two types of cavitation, long living time cavitation and short living time cavitation, in the cavity above pump delivery valve of diesel fuel injection system were observed.

  16. Two-phase flows and heat transfer within systems with ambient pressure above the thermodynamic critical pressure

    Science.gov (United States)

    Hendricks, R. C.; Braun, M. J.; Mullen, R. L.

    1986-01-01

    In systems where the design inlet and outlet pressure P sub amb are maintained above the thermodynamic critical pressure P sub c, it is often assumed that heat and mass transfer are governed by single-phase relations and that two-phase flows cannot occur. This simple rule of thumb is adequate in many low-power designs but is inadequate for high-performance turbomachines, boilers, and other systems where two-phase regions can exist even though P sub amb P sub c. Heat and mass transfer and rotordynamic-fluid-mechanic restoring forces depend on momentum differences, and those for a two-phase zone can differ significantly from those for a single-phase zone. By using a laminar, variable-property bearing code and a rotating boiler code, pressure and temperature surfaces were determined that illustrate nesting of a two-phase region within a supercritical pressure region. The method of corresponding states is applied to bearings with reasonable rapport.

  17. Low Energy Consumption Technique for Processing Rapeseed with Two-phase Extraction System without Ammonia

    Institute of Scientific and Technical Information of China (English)

    Shi Meiren; Qin Jinping; SQian Renyuan

    2000-01-01

    the mother liquid of methanol phase obtained from rapeseed two-phase extraction process can directly be reused in large amounts to process rapeseed again together with some fresh methanol phase, so long as it is treated with a litter of proper additives in advance. The ratio of the reused mother liquid may be more than 3/ 4 of the total amount of the methanol phase needed for processing rapeseed, so the energy consumption can be decreased tremendously. The meal obtained by use of the reused mother liquid keeps the quality as good as those obtained by use of totally fresh methanol phase with light color, weak taste, crispness, good palatability, high protein content, and a low level of glucosinolates much more less than that stipulated by the State for the fodder of grade A.

  18. Mars Aqueous Processing System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Mars Aqueous Processing System (MAPS) is an innovative method to produce useful building materials from Martian regolith. Acids and bases produced from the regolith...

  19. Mars Aqueous Processing System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Mars Aqueous Processing System (MAPS) is a novel technology for recovering oxygen, iron, and other constituents from lunar and Mars soils. The closed-loop...

  20. Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

    Directory of Open Access Journals (Sweden)

    Yollanda Edwirges Moreira Franco

    2011-08-01

    Full Text Available Response surface methodology (RSM based on a five-level, three-variable central composite design (CCD was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.

  1. Quantitative study on experimentally observed poroelastic behavior of Berea sandstone in two-phase fluid system

    Science.gov (United States)

    Goto, Hiroki; Aichi, Masaatsu; Tokunaga, Tomochika; Yamamoto, Hajime; Ogawa, Toyokazu; Aoki, Tomoyuki

    2014-08-01

    Coupled two-phase fluid flow and poroelastic deformation of Berea sandstone is studied through laboratory experiment and numerical simulation. In the experiment, compressed air was infiltrated from the bottom of a water-saturated cylindrical Berea sandstone sample under hydrostatic external stress condition. Both axial and circumferential strains at half the height of the sample showed sudden extension and monotonic and gradual extension afterward. Numerical simulation based on thermodynamically consistent constitutive equations was conducted in order to quantitatively analyze the experimental results. In a simulation assuming isotropy of material properties, the volumetric discharge rate of water at the outlet and one of the axial, circumferential, and volumetric strains at half the height of the sample were reproduced well by each parameter set, while the other two strains were not. When introducing transverse isotropy, all the experimental data were reproduced well. In addition, the effect of saturation dependency of Bishop's effective stress coefficient on the deformation behavior of porous media was discussed, and it was found that strains, both axial and circumferential, are sensitive to the coefficient.

  2. Two-phase (acidogenic-methanogenic) anaerobic thermophilic/mesophilic digestion system for producing Class A biosolids from municipal sludge.

    Science.gov (United States)

    Rubio-Loza, L A; Noyola, A

    2010-01-01

    Two different arrangements of two-phase anaerobic sludge systems were operated treating a mixture of primary and secondary sludge. Two steady state periods were evaluated: the first acidogenic thermophilic phase was operated at hydraulic retention times of 3 and 2 days and the second methanogenic (mesophilic and thermophilic) phases at 13 and 10 days. The two-phase systems had an efficient removal of pathogens and parasites, achieving values lower than those specified for Class A biosolids, according to the Mexican Standard NOM-004-SEMARNAT-2002. The first thermophilic phase achieved almost complete destruction of pathogens and parasites by itself. During the second steady state period, volatile fatty acids accumulated in the second methanogenic phases (HRT of 10 days and an organic load of 3 kg VS/m(3)d) indicating that the systems were overloaded, mainly the mesophilic digester. In this case, the accumulation of propionic acid may be related to a deficiency of micronutrients. The results show that the two-phase thermophilic/mesophilic anaerobic sludge digestion may be considered as an adequate option for the production of Class A biosolids.

  3. Surfactant-coated Candida rugosa Lipase as Catalyst for Hydrolysis of Olive Oil in Solvent-Free Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    宋宝东; 丁辉; 吴金川; HayashiY.; TalukderMMR; 王世昌

    2003-01-01

    The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.

  4. Effect of Mechanical Stress on Magnetic States and Hysteresis Characteristics of a Two-Phase Nanoparticles System

    Directory of Open Access Journals (Sweden)

    Leonid Lazarevich Afremov

    2013-01-01

    Full Text Available In terms of the two-phase nanoparticles model, the effect of mechanical stress on the magnetic state of both uniaxial and multiaxial heterophase magnetic is investigated. The spectrum of critical fields of reversal of phases' magnetic moments was calculated and phase diagrams were drawn to assess the effect of mechanical stress on the degree of metastability of two-phase nanoparticles' magnetic states. By the example of epitaxial cobalt-coated -Fe2O3 particles, a theoretical analysis of the effect of uniaxial mechanical stress on the magnetization of a system of noninteracting heterophase nanoparticles is investigated. It was shown that tension reduced and compression increased coercive force , while the residual saturation magnetization was not changed under the influence of mechanical stress.

  5. Enzymatic synthesis of polyol esters in aqueous-organic two-phase systems.

    OpenAIRE

    Janssen, A.E.M.

    1993-01-01

    The last decade increasingly attention is paid to lipases as catalysts for synthesis of components, such as fatty acid-based surfactants, flavors, edible oil equivalents, monomers and polymers, and amides. In this thesis, the lipase-catalyzed esterification of polyols and fatty acids is described. These esters consist of a nonpolar part (fatty acid) and a polar part (polyol). Therefore, polyol esters have surface-active properties and are used as emulsifier in food, pharmaceutics; and cosmeti...

  6. Partitioning behavior of laccase from Lentinus polychrous Lev in aqueous two phase systems

    Directory of Open Access Journals (Sweden)

    Palapum Khunthongjan

    2012-02-01

    Full Text Available The water current has used as the energy resource for long time however its velocity is very low therefore there arenot found in wide range of uses. This study purposes accelerate water velocity by installing diffuser. The problems wereanalyzed by one dimension analysis and computational fluid dynamics (CFD; the domain covers the diffuser and turbinewhich substituted by porous jump condition is install inside. The flow was identified as axisymmetric steady flow, the inletboundary is identified as uniform flow, all simulation use the same size of diffuser, only the diffuser angles are vary. The resultsshow that velocities of water current in diffuser are increase when the diffuser angle are widen. The angle of diffuser is 20°,the velocity is increase to 1.96 times, compared to free stream velocity. If the angle was about 0-20° and 50-70° the forcetoward diffuser became high instantly; where as the force toward the rotor will be still and the maximum rate of diffuseraugmentation possibly was 3.62 and rotor power coefficient was 2.14.

  7. Enzymatic synthesis of polyol esters in aqueous-organic two-phase systems.

    NARCIS (Netherlands)

    Janssen, A.E.M.

    1993-01-01

    The last decade increasingly attention is paid to lipases as catalysts for synthesis of components, such as fatty acid-based surfactants, flavors, edible oil equivalents, monomers and polymers, and amides. In this thesis, the lipase-catalyzed esterification of polyols and fatty acids is described. T

  8. Slug flow transitions in horizontal gas/liquid two-phase flows. Dependence on channel height and system pressure for air/water and steam/water two-phase flows

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Hideo [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1996-05-01

    The slug flow transitions and related phenomena for horizontal two-phase flows were studied for a better prediction of two-phase flows that typically appear during the reactor loss-of-coolant accidents (LOCAs). For better representation of the flow conditions experimentally, two large-scaled facility: TPTF for high-pressure steam/water two-phase flows and large duct test facility for air/water two-phase flows, were used. The visual observation of the flow using a video-probe was performed in the TPTF experiments for good understanding of the phenomena. The currently-used models and correlations based mostly on the small-scale low-pressure experiments were reviewed and improved based on these experimental results. The modified Taitel-Dukler model for prediction of transition into slug flow from wavy flow and the modified Steen-Wallis correlation for prediction of onset of liquid entrainment from the interfacial waves were obtained. An empirical correlation for the gas-liquid interfacial friction factor was obtained further for prediction of liquid levels at wavy flow. The region of slug flow regime that is generally under influences of the channel height and system pressure was predicted well when these models and correlations were applied together. (author). 90 refs.

  9. Primitive Liquid Water of the Solar System in an Aqueous Altered Carbonaceous Chondrite

    Science.gov (United States)

    Tsuchiyama, A.; Miyake, A.; Kitayama, A.; Matsuno, J.; Takeuchi, A.; Uesugi, K.; Suzuki, Y.; Nakano, T.; Zolensky, M. E.

    2016-01-01

    Non-destructive 3D observations of the aqueous altered CM chondrite Sutter's Mill using scanning imaging x-ray microscopy (SIXM) showed that some of calcite and enstatite grains contain two-phase inclusion, which is most probably composed of liquid water and bubbles. This water should be primitive water responsible for aqueous alteration in an asteroid in the early solar system.

  10. Separated two-phase flow regime parameter measurement by a high speed ultrasonic pulse-echo system.

    Science.gov (United States)

    Masala, Tatiana; Harvel, Glenn; Chang, Jen-Shih

    2007-11-01

    In this work, a high speed ultrasonic multitransducer pulse-echo system using a four transducer method was used for the dynamic characterization of gas-liquid two-phase separated flow regimes. The ultrasonic system consists of an ultrasonic pulse signal generator, multiplexer, 10 MHz (0.64 cm) ultrasonic transducers, and a data acquisition system. Four transducers are mounted on a horizontal 2.1 cm inner diameter circular pipe. The system uses a pulse-echo method sampled every 0.5 ms for a 1 s duration. A peak detection algorithm (the C-scan mode) is developed to extract the location of the gas-liquid interface after signal processing. Using the measured instantaneous location of the gas/liquid interface, two-phase flow interfacial parameters in separated flow regimes are determined such as liquid level and void fraction for stratified wavy and annular flow. The shape of the gas-liquid interface and, hence, the instantaneous and cross-sectional averaged void fraction is also determined. The results show that the high speed ultrasonic pulse-echo system provides accurate results for the determination of the liquid level within +/-1.5%, and the time averaged liquid level measurements performed in the present work agree within +/-10% with the theoretical models. The results also show that the time averaged void fraction measurements for a stratified smooth flow, stratified wavy flow, and annular flow qualitatively agree with the theoretical predictions.

  11. A Novel Flow Measurement System for Cryogenic Two-Phase Flow Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Flow rate measurements for cryogenic propellants are required for spacecraft and space exploration systems. Such a requirement has been hampered by lack of fast and...

  12. Interfacial Area and Interfacial Transfer in Two-Phase Flow Systems (Volume I. Chapters 1-5)

    Energy Technology Data Exchange (ETDEWEB)

    Guo, T.; Park, J.; Kojasoy, G.

    2003-03-15

    Experiments were performed on horizontal air-water bubbly two-phase flow, axial flow, stratified wavy flow, and annular flow. Theoretical studies were also undertaken on interfacial parameters for a horizontal two-phase flow.

  13. Interfacial Area and Interfacial Transfer in Two-Phase Flow Systems (Volume II. Chapters 6-10)

    Energy Technology Data Exchange (ETDEWEB)

    Guo, T.; Park, J.; Kojasoy, G.

    2003-03-15

    Experiments were performed on horizontal air-water bubbly two-phase flow, axial flow, stratified wavy flow, and annular flow. Theoretical studies were also undertaken on interfacial parameters for a horizontal two-phase flow.

  14. Interfacial Area and Interfacial Transfer in Two-Phase Flow Systems (Volume III. Chapters 11-14)

    Energy Technology Data Exchange (ETDEWEB)

    Guo, T.; Park, J.; Kojasoy, G.

    2003-03-15

    Experiments were performed on horizontal air-water bubbly two-phase flow, axial flow, stratified wavy flow, and annular flow. Theoretical studies were also undertaken on interfacial parameters for a horizontal two-phase flow.

  15. Interfacial Area and Interfacial Transfer in Two-Phase Flow Systems (Volume IV. Chapters 15-19)

    Energy Technology Data Exchange (ETDEWEB)

    Guo, T.; Park, J.; Kojasoy, G.

    2003-03-15

    Experiments were performed on horizontal air-water bubbly two-phase flow, axial flow, stratified wavy flow, and annular flow. Theoretical studies were also undertaken on interfacial parameters for a horizontal two-phase flow.

  16. Two-Phase Thermal Switching System for a Small, Extended Duration Lunar Science Platform

    Science.gov (United States)

    Bugby, D.; Farmer, J.; OConnor, B.; Wirzburger, M.; Abel, E.; Stouffer, C.

    2010-01-01

    Issue: extended duration lunar science platforms, using solar/battery or radioisotope power, require thermal switching systems that: a) Provide efficient cooling during the 15-earth-day 390 K lunar day; b) Consume minimal power during the 15-earth-day 100 K lunar night. Objective: carry out an analytical study of thermal switching systems that can meet the thermal requirements of: a) International Lunar Network (ILN) anchor node mission - primary focus; b) Other missions such as polar crater landers. ILN Anchor Nodes: network of geophysical science platforms to better understand the interior structure/composition of the moon: a) Rationale: no data since Apollo seismic stations ceased operation in 1977; b) Anchor Nodes: small, low-power, long-life (6-yr) landers with seismographic and a few other science instruments (see next chart); c) WEB: warm electronics box houses ILN anchor node electronics/batteries. Technology Need: thermal switching system that will keep the WEB cool during the lunar day and warm during the lunar night.

  17. Two-Phase Thermal Switching System for a Small, Extended Duration Lunar Surface Science Platform

    Science.gov (United States)

    Bugby, David C.; Farmer, Jeffery T.; OConnor, Brian F.; Wirzburger, Melissa J.; Abel, Elisabeth D.; Stouffer, Chuck J.

    2010-01-01

    This paper describes a novel thermal control system for the Warm Electronics Box (WEB) on board a small lunar surface lander intended to support science activities anywhere on the lunar surface for an extended duration of up to 6 years. Virtually all lander electronics, which collectively dissipate about 60 W in the reference mission, are contained within the WEB. These devices must be maintained below 323 K (with a goal of 303 K) during the nearly 15-earth-day lunar day, when surface temperatures can reach 390K, and above 263 K during the nearly 15-earth-day lunar night, when surface temperatures can reach 100K. Because of the large temperature swing from lunar day-to-night, a novel thermal switching system was required that would be able to provide high conductance from WEB to radiator(s) during the hot lunar day and low (or negligible) conductance during the cold lunar night. The concept that was developed consists of ammonia variable conductance heat pipes (VCHPs) to collect heat from WEB components and a polymer wick propylene loop heat pipe (LHP) to transport the collected heat to the radiator(s). The VCHPs autonomously maximize transport when the WEB is warm and autonomously shut down when the WEB gets cold. The LHP autonomously shuts down when the VCHPs shut down. When the environment transitions from lunar night to day, the VCHPs and LHP autonomously turn back on. Out of 26 analyzed systems, this novel arrangement was able to best achieve the combined goals of zero control power, autonomous operation, long life, low complexity, low T, and landed tilt tolerance.

  18. Measurement of two-phase particle flow based on the characteristics of particle-system scattering

    Science.gov (United States)

    Li, Min; Zhang, Yang; Wang, Yuan; Yang, Bin

    2017-02-01

    Precise measurement of the mass of moving particles (MPM) is fundamental to the research on particle flows. In non-intrusive optical measurements, traditional discrete methods obtain the MPM by determining the connected domains on experimental pictures, leading to inevitable errors. In this study, the constitutive relationship between the MPM and the foreground grey scale value of the experimental picture is investigated on the basis of the working principle of digital cameras and the scattering theory of particle systems. This relationship is confirmed to be effective in wind-blown sand particle flow experiments using high-speed photography.

  19. Numerical Simulation of One- and Two-Phase Flows in Propulsion Systems

    Science.gov (United States)

    Gilinsky, Mikhail; Verma, Arun; Hardin, Jay C.; Banerjee, Debrup; Blankson, Isaiah M.; Hendricks, Robert C.; Shvets, Alexander I.

    2003-01-01

    Four subprojects were conducted using analytical methods, numerical simulation and experimental tests: (A) Shock wave mitigation by spike-shaped blunt bodies with application for the purpose of drag, lift and longitudinal momentum optimization. The main result in this subproject is: application of a single needle against a supersonic flow provides higher benefits for blunt body drag reduction and heat transfer to the body than the application of multiple needles. (B) Solid particles, liquid and air jet injection through the front of a blunt body against a supersonic flow. In this case, the research conducted and analysis of multiple previous investigations in this area have shown essential benefits and preferable application of solid particle injection. (C) Comparison of different methods of fuel injection into supersonic duct flows. Preliminary numerical simulations and theoretical analysis show promising results for Telescope-shaped inlet applications in SCRAMJET; and (D) Development of an acoustic source location method for different applications including propulsion systems.

  20. Comparative performance and microbial community of single-phase and two-phase anaerobic systems co-digesting cassava pulp and pig manure

    DEFF Research Database (Denmark)

    Panichnumsin, P.; Ahring, B.K.; Nopharatana, A.

    2010-01-01

    In this study, we illustrated the performance and microbial community of single- and two-phase systems anaerobically co-digesting cassava pulp and pig manure. The results showed that the volatile solid reduction and biogas productivity of two-phase CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, whi...

  1. Comparative assessment of olive oil mill effluents from three-phase and two-phase systems, treated for hydrogen production.

    Science.gov (United States)

    Rouvalis, A; Iliopoulou-Georgudaki, J

    2010-10-01

    By-products of a two-phase and a three-phase olive oil mill process treated in an anaerobic fermentation system for hydrogen production, were evaluated by three bioassays: the zebrafish Danio rerio embryo test and two microbiotests, Thamnotoxkit F and Daphtoxkit F™ pulex. Samples from both processes were classified as "very toxic" with LC(50) values ranging from 1.52% (T. platyurus 24 h test) to 4.48% (D. pulex 48 h-LC₅₀). Toxicity values were differently correlated to physicochemical parameters showing different degree of influence. The treated effluents of both process systems remained very toxic showing the necessity for further treatment, aiming to environmentally safe discharges.

  2. Energetic and exploitation characteristics of a two-phase ejecting vacuum system built in the paper machine at A.D. Komuna Skopje

    Directory of Open Access Journals (Sweden)

    Šarevski Milan

    2004-01-01

    Full Text Available This work presents the concept of a two-phase ejecting vacuum system. The energetic characteristics of these systems were analyzed and compared to those with mechanical pumps with water ring and turbo compressor vacuum pumps. A method for optimizing a two-phase thermo compressor vacuum systems is presented. The analysis included the exploitation characteristics of the system developed at A.D. "Komuna" Skopje.

  3. Aqueous Two-phase Extraction of Resveratrol from Red Grape Seed%双水相萃取双优红葡萄籽中的白藜芦醇

    Institute of Scientific and Technical Information of China (English)

    曹雪杰; 高明波; 朴晶晶; 廖颖玲

    2015-01-01

    The wine making process produces a large quantity of grape secondary products, which include grape seeds, skins, and stems and so on. These byproducts comprise approximately 20% of the harvested grapes. All the byproducts are only scraps from the grape wine, generally treated as waste. Resveratrol, exists mainly in the above byproducts, owning a number of functions, such as antibacterial, anti-cancer, anti-oxidation function. It can also inhibit platelet aggregation, bring vasodilation and improve the body immunity. The method of aqueous two-phase extraction was used to extract the resveratrol in the grape Shuangyouhong seeds. The whole extraction is green and simple, which was far better than the traditional organic solvent extraction. Of the 16 kinds of aqueous two-phase systems, the best one of ethanol/ dipotassium phosphate system was optimized. On the basis of single factor experiment and the following four factors level contrast experiment, the extraction conditions of resveratrol were optimized as follows: 70% ethanol, solid to liquid ratio of 1:15, extraction for 2. 5 h at 60 ℃. The content of resveratrol in the Shuangyouhong grape seeds was 0. 484%.%葡萄酿酒过程中会产生大量的副产品,主要包括:葡萄籽,葡萄皮和葡萄茎;这些副产品约占葡萄总成分的20%。这些副产品是葡萄酿酒后的下脚料,一般被当做废弃物。白藜芦醇却主要存在于以上副产品中。其具有抗菌、抗癌、抗氧化、抑制血小板凝集和舒张血管、提高机体免疫力等特性。本实验利用双水相萃取的方法萃取“双优红”葡萄籽中的白藜芦醇,萃取过程绿色简洁,比传统有机溶剂萃取法有更好的萃取效果。从16种双水相体系中优选出最佳双水相体系乙醇/磷酸氢二钾。在单因素实验基础上,通过四因素水平对比实验优化白藜芦醇的萃取条件为:料液比为1:15,温度为60℃,乙醇萃取浓度为70%,萃取时间为2.5 h,在此最

  4. Modeling and investigation of refrigeration system performance with two-phase fluid injection in a scroll compressor

    Science.gov (United States)

    Gu, Rui

    Vapor compression cycles are widely used in heating, refrigerating and air-conditioning. A slight performance improvement in the components of a vapor compression cycle, such as the compressor, can play a significant role in saving energy use. However, the complexity and cost of these improvements can block their application in the market. Modifying the conventional cycle configuration can offer a less complex and less costly alternative approach. Economizing is a common modification for improving the performance of the refrigeration cycle, resulting in decreasing the work required to compress the gas per unit mass. Traditionally, economizing requires multi-stage compressors, the cost of which has restrained the scope for practical implementation. Compressors with injection ports, which can be used to inject economized refrigerant during the compression process, introduce new possibilities for economization with less cost. This work focuses on computationally investigating a refrigeration system performance with two-phase fluid injection, developing a better understanding of the impact of injected refrigerant quality on refrigeration system performance as well as evaluating the potential COP improvement that injection provides based on refrigeration system performance provided by Copeland.

  5. Immobilized metal-ion affinity partitioning of NAD(+)-dependent dehydrogenases in poly(ethylene glycol)-dextran two-phase systems.

    Science.gov (United States)

    Pesliakas, H; Zutautas, V; Baskeviciute, B

    1994-08-26

    Affinity partitioning of yeast alcohol dehydrogenase (YADH), lactate dehydrogenase from rabbit muscle (MLDH) and lactate and malate dehydrogenases from pig heart (HLDH and HMDH, respectively) were studied in aqueous two-phase systems containing metal ions (Cu2+, Ni2+, Zn2+ and Cd2+) chelated by iminodiacetate-poly(ethylene glycol) (IDA-PEG). The partitioning behaviour of the enzymes in the presence of Cu(II)-IDA-PEG was studied as a function of the concentration of NaCl, the pH of the medium and the concentration of added selected agents. It was demonstrated that the partition effect (delta log K) of dehydrogenases in the presence of Cu(II)-IDA-PEG and the affinity of enzymes for immobilized Cu2+ ions increases in the order MLDH > YADH > HMDH > or = HLDH. It was shown that the determined variations in the enzyme affinities for Cu(II)-IDA-PEG might be related to the differences in the content of histidine residues accessible to the solvent.

  6. Two phase sampling

    CERN Document Server

    Ahmad, Zahoor; Hanif, Muhammad

    2013-01-01

    The development of estimators of population parameters based on two-phase sampling schemes has seen a dramatic increase in the past decade. Various authors have developed estimators of population using either one or two auxiliary variables. The present volume is a comprehensive collection of estimators available in single and two phase sampling. The book covers estimators which utilize information on single, two and multiple auxiliary variables of both quantitative and qualitative nature. Th...

  7. Study on the Intermediate in the o-Phenylenediamine Oxidative Reaction Using Hemoglobin as A Mimetic Peroxidase in Aqueous-Organic Two Phase

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Hemoglobin was used as a mimetic enzyme for peroxidase to catalyze the oxidative reaction ofo-phenylenediamine with H2O2 which functioned as an oxidant. The relationship between physicochemicalproperties of the intermediate and enzymatic activity of hemoglobin was studied. Since the solubility of theintermediate in the reaction is higher in butanol phase than in water phase, the intermediate itself diffusedfrom the aqueous phase to the butanol phase. The experimental results showed that the rate of product andthe stability of intermediate were associated with the temperature and the pH value of the buffer. The for-mation rate of intermediate and half-life period reveal the maximal in pH7, nevertheless, the whole rate ofthe catalytic reaction is the greatest in pH5, which the ratio of the initial rate in final product formationcompared to that intermediate formation is the greatest.

  8. Evidence for Two Phases of Galaxy Formation from Radial Trends in the Globular Cluster System of NGC 1407

    CERN Document Server

    Forbes, Duncan; Strader, Jay; Romanowsky, Aaron; Brodie, Jean; Foster, Caroline

    2011-01-01

    Here we present the colours of individual globular clusters (GCs) around the massive elliptical galaxy NGC 1407 out to a projected galactocentric radius of 140 kpc or 17 galaxy effective radii (R$_e$). Such data are a proxy for the halo metallicity. We find steep, and similar, metallicity gradients of ~ -0.4 dex per dex for both the blue (metal-poor) and red (metal-rich) GC subpopulations within 5-8.5 R_e (40-70 kpc). At larger radii the mean GC colours (metallicity) are constant. A similar behaviour is seen in a wide-field study of M87's GC system, and in our own Galaxy. We interpret these radial metallicity trends to indicate an inner region formed by early in-situ dissipative processes and an outer halo formed by ongoing accretion of low mass galaxies and their GCs. These results provide observational support for the model of galaxy formation whereby massive galaxies form inside-out in two phases. We have also searched the literature for other massive early-type galaxies with reported GC metallicity gradie...

  9. High-calorific biogas production from anaerobic digestion of food waste using a two-phase pressurized biofilm (TPPB) system.

    Science.gov (United States)

    Li, Yeqing; Liu, Hong; Yan, Fang; Su, Dongfang; Wang, Yafei; Zhou, Hongjun

    2017-01-01

    To obtain high calorific biogas via anaerobic digestion without additional upgrading equipment, a two-phase pressurized biofilm system was built up, including a conventional continuously stirred tank reactor and a pressurized biofilm anaerobic reactor (PBAR). Four different pressure levels (0.3, 0.6, 1.0 and 1.7MPa) were applied to the PBAR in sequence, with the organic loading rate maintained at 3.1g-COD/L/d. Biogas production, gas composition, process stability parameters were measured. Results showed that with the pressure increasing from 0.3MPa to 1.7MPa, the pH value decreased from 7.22±0.19 to 6.98±0.05, the COD removal decreased from 93.0±0.9% to 79.7±1.2% and the methane content increased from 80.5±1.5% to 90.8±0.8%. Biogas with higher calorific value of 36.2MJ/m(3) was obtained at a pressure of 1.7MPa. Pressure showed a significant effect on biogas production and gas quality in methanogenesis reactor.

  10. Numerical modelling of the impact of a liquid drop on the surface of a two-phase fluid system

    Science.gov (United States)

    Sochan, Agata; Lamorski, Krzysztof; Bieganowski, Andrzej; Ryżak, Magdalena

    2014-05-01

    The aim of the study was validation of a numerical model of the impact of a liquid drop on the surface of a two-phase system of immiscible fluids. The drop impact phenomenon was recorded using a high-speed camera (Vision Research MIRO M310) and the data were recorded at 2000 frames per second. The numerical calculations were performed with the Finite Volume Method (FVM) solving the three-dimensional Navier-Stokes equations for three phases: air and two selected immiscible fluids. The Volume of Fluid (VOF) technique was employed for modelling of the boundaries between the phases. Numerical modelling was done with the Finite Volume Method using an available OpenFOAM software. The experiment was based on three variables: • the height from which the drop of the selected fluids fell (the speed of the drop), • the thickness of the layers of the two selected immiscible fluids (a thin layer of the fluid with a lower density was spread over the higher-density fluid), • the size of the fluid droplet. The velocity and radius of the falling drop was calculated based on the recorded images. The used parameters allowed adequate projection of the impact of fluid droplets on a system of two immiscible liquids. Development of the numerical model of splash may further have practical applications in environmental protection (spraying of hazardous fluids, spread of fuels and other hazardous substances as a result of disasters, spraying (water cooling) of hot surfaces), and in agriculture (prevention of soil erosion). The study was partially funded from the National Science Centre (Poland) based on the decision no. DEC-2012/07/N/ST10/03280.

  11. Enabling two-phase microfluidic thermal transport systems using a novel thermal-flux degassing and fluid charging approach

    Science.gov (United States)

    Singh Dhillon, Navdeep; Pisano, Albert P.

    2014-03-01

    A novel two-port thermal-flux method has been proposed and demonstrated for degassing and charging two-phase microfluidic thermal transport systems with a degassed working fluid. In microscale heat pipes and loop heat pipes (mLHPs), small device volumes and large capillary forces associated with smaller feature sizes render conventional vacuum pump-based degassing methods quite impractical. Instead, we employ a thermally generated pressure differential to purge non-condensable gases from these devices before charging them with a degassed working fluid in a two-step process. Based on the results of preliminary experiments studying the effectiveness and reliability of three different high temperature-compatible device packaging approaches, an optimized compression packaging technique was developed to degas and charge a mLHP device using the thermal-flux method. An induction heating-based noninvasive hermetic sealing approach for permanently sealing the degassed and charged mLHP devices has also been proposed. To demonstrate the efficacy of this approach, induction heating experiments were performed to noninvasively seal 1 mm square silicon fill-hole samples with donut-shaped solder preforms. The results show that the minimum hole sealing induction heating time is heat flux limited and can be estimated using a lumped capacitance thermal model. However, further continued heating of the solder uncovers the hole due to surface tension-induced contact line dynamics of the molten solder. It was found that an optimum mass of the solder preform is required to ensure a wide enough induction-heating time window for successful sealing of a fill-hole.

  12. The formation and physical stability of two-phase solid dispersion systems of indomethacin in supercooled molten mixtures with different matrix formers.

    Science.gov (United States)

    Semjonov, Kristian; Kogermann, Karin; Laidmäe, Ivo; Antikainen, Osmo; Strachan, Clare J; Ehlers, Henrik; Yliruusi, Jouko; Heinämäki, Jyrki

    2017-01-15

    Amorphous solid dispersions (SDs) are a promising approach to improve the dissolution rate of and oral bioavailability of poorly water-soluble drugs. In some cases multi-phase, instead of single-phase, SD systems with amorphous drug are obtained. While it is widely assumed that one-phase amorphous systems are desirable, two-phase systems may still potentially exhibit enhanced stability and dissolution advantages over undispersed systems. The objective of the present study was to understand the solid-state properties of two-phase SDs with amorphous drug and their relation to physical stability. Two different types of excipients for SD formation were used, one being a polymer and the other a small molecule excipient. The supercooled molten SDs of a poorly water-soluble indomethacin (IND) with a graft copolymer, Soluplus® (SOL) and sugar alcohol, xylitol (XYL) were prepared. Supercooled molten SDs of IND with SOL were two-phase glassy suspension in which the amorphous drug was dispersed in an amorphous polymer matrix. A short-term aging of the SDs led to the formation of glassy suspensions where the crystalline drug was dispersed in an amorphous polymer matrix. These were physically stable at room temperature for the time period studied (RT, 23±2°C), but aging at high-humidity conditions (75% RH) recrystallization to metastable α-IND occurred. Interestingly, the SDs with XYL were two-phase amorphous precipitation systems in which the drug was in an amorphous form in the crystalline sugar alcohol matrix. The SDs of IND and XYL exhibited fast drug recrystallization. In conclusion, the preparation method of two-phase systems via co-melting in association with the rapid quench cooling is a feasible method for the formulation of poorly water-soluble drugs. The physical stability of these two-phase systems, however, is dependent on the carrier material and storage conditions.

  13. Thermal-gravitational modeling and scaling of two-phase heat transport systems from micro-gravity to super-gravity levels

    Science.gov (United States)

    Delil, A. A. M.

    2001-02-01

    Earlier publications extensively describe NLR research on thermal-gravitational modeling and scaling of two-phase heat transport systems for spacecraft applications. These publications on mechanically and capillary pumped two-phase loops discuss pure geometric scaling, pure fluid to fluid scaling, and combined (hybrid) scaling of a prototype system by a model at the same gravity level, and of a prototype in micro-gravity environment by a scale-model on earth. More recent publications include the scaling aspects of prototype two-phase loops for Moon or Mars applications by scale-models on earth. Recent work, discussed here, concerns extension of thermal-gravitational scaling to super-g acceleration levels. This turned out to be necessary, since a very promising super-g application for (two-phase) heat transport systems will be cooling of high-power electronics in spinning satellites and in military combat aircraft. In such aircraft, the electronics can be exposed during maneuvres to transient accelerations up to 120 m/s2. The discussions focus on ``conventional'' (capillary) pumped two-phase loops. It can be considered as introduction to the accompanying article, which focuses on pulsating and oscillating devices. .

  14. (乙醇+丙酮)-硫酸铵二元双水相分离萃取盐酸四环素%Ethanol and acetone-ammonium sulfate binary aqueous two-phase extraction separation of tetracycline

    Institute of Scientific and Technical Information of China (English)

    关卫省; 邓江华; 韩娟

    2013-01-01

    基于小分子醇双水相体系和小分子有机物双水相体系,建立了(乙醇+丙酮)-(NH4)2SO4形成的二元双水相体系萃取盐酸四环素的新方法.研究了分相盐、醇及小分子有机物的类型、用量及比例、温度、溶液酸度、共存物质对盐酸四环素分配行为的影响.结果表明,硫酸铵浓度为41%、无水乙醇与丙酮体积比为2:1、溶液酸度在pH 4.5-5范围、温度为25℃二元双水相体系对四环素有较高萃取率可达90%,分配系数达到57.当测试允许相对误差为±7%时,浓度为75 mg·L-1的盐酸四环素,K+、Na+、F-、Cl-、NO3-、Br-浓度均为10%对测定结果无干扰,1.5倍的盐酸美他环素、35倍的氯霉素、青霉素和红霉素、80倍的淀粉和蔗糖、400倍的葡萄糖和果糖对测定结果不发生干扰.%Based on small molecule alcohol and organic molecule aqueous two -phase system, a new method was developed for extraction of tetracycline hydrochloride in ethanol-acetone-ammonium sulfate binary aqueous system. Several factors on the tetracycline hydrochloride allocation behavior was studied, including the type, amounts and relative proportions of salt-alcohol-small molecular organics, temperature, pH and the coexisting substances. The results showed that when ammonium sulfate was 41% , the volume ratio of absolute ethyl alcohol and acetone was 2:1, and the solution pH was 4. 5-5, the extraction rate and the distribution coefficient of tetracycline hydrochloride were 90% and 57 respectively. The relative error was ±7% , the ions including K+ , Na+ , F- , Cl- , NO3- , Br- , the hydrochloride metacycline, and other components in the system such as chloramphenicol penicillin, erythromycin, starch and sucrose, glucose and fructose have not any effect on the extraction behavior.

  15. 天花粉蛋白的盐析工艺和亲和双水相分离工艺%Salting-out process and affinity aqueous two-phase extraction process of trichosanthin

    Institute of Scientific and Technical Information of China (English)

    汪苗苗; 丁玉

    2012-01-01

    In recent years, clinical studies showed that trichosanthin's has anti-cancer and anti- HIV virus function. The trichosanthin's production needs to be studied to meet the market needs. This experiment used two steps of tricho-santhin extraction a) purification trichosanthin with ammonium sulfate precipitation, b) affinity aqueous two-phase extraction after ammonium sulfate coarse separation. The extraction process was optimized and gel electrophoresis was used in identifying the purity and molecular weight. The salting-out method and affinity aqueous two-phase separation method were compared. The best conditions for salting-out separation were: solid-liquid ratio 1:12, soaking time 24 hours, pH 5, and the yield 0. 8ml/g. The best affinity aqueous two-phase separation conditions were; the percentage of Cibacron Blue F3GA-Tween 80 was 8% , the content of Tween 80 in aqueous two-phase was 4. 0% , content of ( NH4 )2SO4 was 2.0mol/L, mixing time 9h, stripping sodium content 0. 5mol/L, the anti-extraction time 8h, yield was 0.6mg/g. The electrophoresis showed the products had a high purity.%近年,随着天花粉蛋白(TCS)在抗癌细胞和HIV病毒方面的临床性研究进展,天花粉蛋白生产工艺也需在保证效益的前提下精细化.本实验利用硫酸铵分级沉淀、硫酸铵粗提后亲和双水相进一步纯化,得到精制的天花粉蛋白.并对提取工艺进行了优化,最后利用SDS聚丙烯酰胺凝胶电泳对其纯度进行了鉴定以及相对分子质量的测定,并对比了盐析法和亲和双水相分离法,研究结果表明:硫酸铵沉淀法最佳分离条件为固液比为1∶12、浸泡时间为24h、pH在5左右,最终得率0.8mg/g;亲和萃取最佳条件:染料CibacronBlue F3GA-吐温80在成相吐温80中的含量8%,成相吐温80的占双水相含量为4.0%,(NH4)2S04的含量2.0mol/L,充分混合的时间9h,反萃取氯化钠的含量为0.5mol/L,反萃取的时间为8h,得率0.6mg/g,但凝胶电泳显示其纯度较高.

  16. Optimization of ultrasound-assisted aqueous two-phase extraction of polyphenols from mango seed kernel%超声辅助双水相集成提取芒果核多酚工艺优化

    Institute of Scientific and Technical Information of China (English)

    高云涛; 王振峰; 罗利军; 戴建辉; 李鼎

    2012-01-01

    为了获得超声辅助双水相集成提取芒果核多酚的最佳条件,该文提出一种超声提取与丙醇-硫酸铵双水相分离体系集成提取芒果核多酚的新方法,利用5水平4因素的星点设计响应面方法研究提取条件对芒果核总多酚提取率的影响.当超声功率为400W、初始温度为45℃,获得的最佳条件是59.7%丙醇浓度,23.4%硫酸铵用量,27.3 min超声提取时间和23.0 mL/g的液固比,在该条件下,芒果核多酚提取率为6.67%,试验值与最佳预测值(6.82%)相符,超声辅助双水相集成法获得的提取物多酚含量高于常规超声提取法.试验结果说明利用超声辅助双水相集成的方法改善多酚提取是可行性.该研究可为芒果核的利用和植物多酚的提取技术提供参考.%In order to obtain the optimal conditions of ultrasound-assisted aqueous two-phase extraction of polyphenols from mango seed kernel,a novel extraction method involving ultrasound-assisted extraction combined with propyl-alcohol ammonium sulfate aqueous two-phase separation was developed for the extraction of polyphenols from mango seed kernel.Response surface methodology,based on a five levels,four variables central composite designs was employed to study the effect of extraction conditions on the extraction yield of total polyphenols.An extraction yield of 6.67% was achieved under the optimal extraction conditions of 59.7% of propyl-alcohol volume fraction,23.4% of ammonium sulfate investigated concentration,27.0 min of extraction time and liquid/solid ratio of 23 mL/g at ultrasonic power of 400 W and temperature of 45℃,which was agreement well with the optimal prediction value (6.82%).Totalpolyphenols content in the extract of ultrasound-assisted aqueous two-phase extraction was higher than that of conventional ultrasound-assisted extraction.The research indicates that the method of combining aqueous two-phase separation with ultrasound-assisted extraction to

  17. 用巨大芽孢杆菌ECU1001环氧水解酶在有机—水两相系统中高效生产(S)-缩水甘油苯基醚%Efficient Production of Enantiopure (S)-Glycidyl Phenyl Ether by an Epoxide Hydrolase from Bacillus megaterium ECU1001 in an Organic-Aqueous Two-Phase System

    Institute of Scientific and Technical Information of China (English)

    宫鹏飞; 许建和

    2002-01-01

    @@ Microbial epoxide hydrolases are highly versatile catalysts for the asymmetric hydrolysis of chiral epoxides, which are extensively employed as useful building blocks for the synthesis of various biologically active products in the pharmaceutical and agrochemical industries. Glycidyl phenyl ether (GPE) is one of the potentially useful aryloxy epoxides for the synthesis of chiral amino alcohols[1] and bioactive compounds such as β-blockers[2]. In our laboratory, a strain of Bacillus megaterium ECU1001, which produces a novel epoxide hydrolase for kinetic resolution of (R,S)-GPE with high enantioselectivity, was successfully isolated from soil for the first time and proved to be useful for chiral synthesis in an aqueous phase[3,4]. However, most epoxides including GPE can be spontaneously hydrolyzed into their vicinal diols without any enantioselectivity in an aqueous phase. In addition, the instability and low solubility of epoxides in a single aqueous phase may result in a marked decrease in the yield of kinetic resolution[5], thus limiting the application of these resolution processes on an industrial scale.

  18. SDS/[C_nmim]Br/H_2O体系的双水相性质%Aqueous Two-Phase System(ATPS) Containing Ionic Liquids [C_nmim]Br and SDS

    Institute of Scientific and Technical Information of China (English)

    陈婷; 马爱青; 王菲; 尚亚卓; 刘洪来

    2012-01-01

    研究了咪唑类离子液体[Cnmim]Br与传统阴离子表面活性剂SDS混合水溶液的性质,结果显示混合水溶液性质随咪唑阳离子上烷烃链长的变化而呈现出明显的不同。当离子液体取代烷基链的碳数大于或等于6时,混合体系可以自发地分为共存的两相,即双水相。共存的两相界面清晰、性能稳定且能有效萃取染料二甲酚橙,有望成为新型高效的分离体系,在生物活性物质的纯化、分离中发挥作用。混合体系中离子液体和传统表面活性剂相对含量的改变引起混合溶液中表面活性剂聚集体尺寸和形态的变化,最终导致双水相上、下两相表观现象的差异。此外,离子液体、SDS头基间库仑力作用、烷烃链的疏溶剂力以及离子液体与SDS的协同效应,是形成溶致液晶的重要驱动力,导致较低浓度下十字花纹理层状液晶(LC)的生成。%Properties of mixed aqueous solutions of ionic liquid(Br) and traditional surfactant(SDS) were studied.Results showed that the properties of the mixed systems varied with the chain length of substituted alkyl in ionic liquids.Only those systems containing ionic liquids with equal to or more than 6 carbon atoms in alkyl chains can form aqueous two phase system(ATPS).The ATPS containing ionic liquids and surfactant has obvious extraction ability to xylenol orange and the potential to separate and purify biomaterials.The difference in appearance of ATPS should be attributed to the various size and shape of the surfactants aggregates formed in mixed solutions.Furthermore,the electrostatic attractive interaction,the hydrophobic interaction in addition to the synergistic effect between ionic liquids and SDS further promote the surfactant assembly,which leads to the formation of liquid crystal at lower surfactant concentration.

  19. Maximizing the utilization of Laminaria japonica as biomass via improvement of alginate lyase activity in a two-phase fermentation system.

    Science.gov (United States)

    Oh, Yuri; Xu, Xu; Kim, Ji Young; Park, Jong Moon

    2015-08-01

    Brown seaweed contains up to 67% of carbohydrates by dry weight and presents high potential as a polysaccharide feedstock for biofuel production. To effectively use brown seaweed as a biomass, degradation of alginate is the major challenge due to its complicated structure and low solubility in water. This study focuses on the isolation of alginate degrading bacteria, determining of the optimum fermentation conditions, as well as comparing the conventional single fermentation system with the two-phase fermentation system which is separately using alginate and mannitol extracted from Laminaria japonica. Maximum yield of organic acids production and volatile solids reduction obtained were 0.516 g/g and 79.7%, respectively, using the two-phase fermentation system in which alginate fermentation was carried out at pH 7 and mannitol fermentation at pH 8. The two-phase fermentation system increased the yield of organic acids production by 1.14 times and led to a 1.45-times reduction of VS when compared to the conventional single fermentation system at pH 8. The results show that the two-phase fermentation system improved the utilization of alginate by separating alginate from mannitol leading to enhanced alginate lyase activity.

  20. Increased production of megakaryocytes near purity from cord blood CD34+ cells using a short two-phase culture system.

    Science.gov (United States)

    Boyer, Lucie; Robert, Amélie; Proulx, Chantal; Pineault, Nicolas

    2008-03-20

    Expansion of hematopoietic progenitor cells (HPC) ex vivo remains an important focus in fundamental and clinical research. The aim of this study was to determine whether the implementation of such expansion phase in a two-phase culture strategy prior to the induction of megakaryocyte (Mk) differentiation would increase the yield of Mks produced in cultures. Toward this end, we first characterized the functional properties of five cytokine cocktails to be tested in the expansion phase on the growth and differentiation kinetics of CD34+-enriched cells, and on their capacity to expand clonogenic progenitors in cultures. Three of these cocktails were chosen based on their reported ability to induce HPC expansion ex vivo, while the other two represented new cytokine combinations. These analyses revealed that none of the cocktails tested could prevent the differentiation of CD34+ cells and the rapid expansion of lineage-positive cells. Hence, we sought to determine the optimum length of time for the expansion phase that would lead to the best final Mk yields. Despite greater expansion of CD34+ cells and overall cell growth with a longer expansion phase, the optimal length for the expansion phase that provided greater Mk yield at near maximal purity was found to be 5 days. Under such settings, two functionally divergent cocktails were found to significantly increase the final yield of Mks. Surprisingly, these cocktails were either deprived of thrombopoietin or of stem cell factor, two cytokines known to favor megakaryopoiesis and HPC expansion, respectively. Based on these results, a short resource-efficient two-phase culture protocol for the production of Mks near purity (>95%) from human CD34+ CB cells has been established.

  1. Metal separations using aqueous biphasic partitioning systems

    Energy Technology Data Exchange (ETDEWEB)

    Chaiko, D.J.; Zaslavsky, B.; Rollins, A.N.; Vojta, Y.; Gartelmann, J.; Mego, W. [Argonne National Lab., IL (United States). Chemical Technology Div.

    1996-05-01

    Aqueous biphasic extraction (ABE) processes offer the potential for low-cost, highly selective separations. This countercurrent extraction technique involves selective partitioning of either dissolved solutes or ultrafine particulates between two immiscible aqueous phases. The extraction systems that the authors have studied are generated by combining an aqueous salt solution with an aqueous polymer solution. They have examined a wide range of applications for ABE, including the treatment of solid and liquid nuclear wastes, decontamination of soils, and processing of mineral ores. They have also conducted fundamental studies of solution microstructure using small angle neutron scattering (SANS). In this report they review the physicochemical fundamentals of aqueous biphase formation and discuss the development and scaleup of ABE processes for environmental remediation.

  2. Study on constant-pressure specific heat of non-equi- librium phase change process in gas-liquid two-phase flow system

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In this paper, the air-water vapor-water system is taken as an example, and the formula of constantpressure specific heat during non-equilibrium phase change process in the two-phase flow system is deduced using the theory of two-phase flow and thermophysics. The constant-pressure specific heat of non-equilibrium phase change process is calculated with the corresponding numerical model, and the numerical results are compared to those of the equilibrium phase change process. It is shown that in evaporation process, the variational rate of the non-equilibrium specific heat increases with increasing initial fluid temperature and particle mass fraction. The smaller particle radius is, the faster the variational rate is. Meanwhile, the constant-pressure specific heat of equilibrium process is higher than that of the non-equilibrium process all the time.

  3. Measurement and simulation of the turbulent dispersion of a radioactive tracer in a two-phase flow system

    Energy Technology Data Exchange (ETDEWEB)

    Hensel, F.; Rohde, U.

    1998-10-01

    The turbulent dispersion of a radiotracer in an experimental setup with a natural convection liquid-gaseous flow was investigated. A liquid-gaseous bubbly flow was generated in a narrow tank by injection of pressurized air into water or by catalytic disintegration of H{sub 2}O{sub 2}. Turbulent Prandtl numbers for gas and tracer dispersion were varied. In the case of higher gas superficial velocities (J{sub gas}{approx}5-15 mm/s), a reasonable agreement was achieved between calculated and measured tracer transport velocity and dispersion coefficient values. A nearly linear correlation between j{sub gas} and D was found in agreement with other authors. The calculation results contribute to a better understanding of the phenomena and interpretation of the measurement results as well as to the validation of the CFD code for turbulent two-phase flow applications. Further investigations are necessary to improve the agreement in the cases of H{sub 2}O{sub 2} disintegration and low gas superficial velocities. (orig.)

  4. 乙醇-硫酸铵双水相萃取镉-碘化钾-罗丹明B离子缔合物%Extraction of cadmium-potassium iodide-rhodamine B ion association complex by alcohol-ammonium sulfate aqueous two-phase

    Institute of Scientific and Technical Information of China (English)

    曾云; 覃事栋; 姚康康; 王影; 肖苗

    2012-01-01

    In the presence of ammonium sulphate, the extraction behavior of Cd (Ⅱ) based on potassium iodide-Rhodamine B-alcohol system was studied and the optimal condition of phase separation was selected. Results showed that in the pH1 ~3 media, the extraction rate of [CdI4]2- by alcohol-ammonium sulfate aqueous two-phase system is only 35.5% , and the ion association complex, which is formed by adding rhodamine B, can be extracted completely by the aqueous two-phase system. In the system, Cd2+ can be dissociated completely from Zn2+, Fe3+, Co2+, Cu2+, and Ni2+.%研究了在( NH4) 2SO4存在下,碘化钾-罗丹明B-乙醇体系萃取Cd(Ⅱ)的行为及最佳分相条件.实验表明,在pH 1~3时,乙醇-( NH4 )2SO4双水相体系对[ CdI4]2-络阴离子的萃取率只有35.5%;加入罗丹明B后,该体系能完全萃取镉-碘化钾-罗丹明B形成的离子缔合物,而干扰离子Zn2+、Fe3+、Co2+、Cu2+、Ni2+不被萃取,实现Cd2+与上述离子的分离.

  5. Predicting the influence of compressibility and thermal and flow distribution asymmetry on the frequency-response characteristics of multitube two-phase condensing flow systems

    Energy Technology Data Exchange (ETDEWEB)

    Kobus, C.J.; Wedekind, G.L.; Bhatt, B.L.

    2000-02-01

    An equivalent single-tube model concept was extended to predict the frequency-response characteristics of multitube two-phase condensing flow systems, complete with the ability to predict the influence of compressibility and thermal and flow distribution asymmetry. The predictive capability of the equivalent single-tube model was verified experimentally with extensive data that encompassed a three-order-of-magnitude range of frequencies, and a wide range of operating parameters.

  6. An Experimental Study of Air-Solid Two-Phase Flow in a 90° Bend Using LDV System

    Institute of Scientific and Technical Information of China (English)

    1992-01-01

    The measurements of he mean streamwise and radial velocities,the associated turbulence and the relative particle densities were made in an air-solid two-phase flow in square sectioned(30mm×30mm) 90° vertical to horizontal bend using laser Doppler velocimetry.The radius ratio of the bedn was 2.0.Glass beads of 100um in diameter were employed to form the solid phase.The measurements of air and solid phases were performed separately at the same bulk velocity 19.34m/s,correaponding to a Reynolds number of 3.87×104.The mass ratio of solid to air was 1.6%,The results indicate that the particle trajectories are very close to straight lines.The streamwise velocity profiles for the gas and the solids cross over near the outer wall with the solids having the higher speed.At θ=30° and 45°,particle-wall collisions happen mostly in the region fromθ=30°to θ=75°,and cause a sudden change in solid velocity,The particles tned to move towards the outer wall in 90°bend,The particle concentration near the outer wall is umch higher than that near the inner wall in the bend,and there are few particles in the inside of the bend.The bend leads to apparent phase separation.atθ=45°,the solids concentrate in the half of the duct near the outer wall,After θ=60° the second peak concentration appears,and goes gradually towards the inner wall.

  7. Experimental and CFD-PBM approach coupled with a simplified dynamic analysis of mass transfer in phenol biodegradation in a three phase system of an aerated two-phase partitioning bioreactor for environmental applications

    Science.gov (United States)

    Moradkhani, Hamed; Anarjan Kouchehbagh, Navideh; Izadkhah, Mir-Shahabeddin

    2016-07-01

    A three-dimensional transient modeling of a two-phase partitioning bioreactor, combining system hydrodynamics, two simultaneous mass transfer and microorganism growth is modeled using computational fluid dynamics code FLUENT 6.2. The simulation is based on standard "k-ɛ" Reynolds-averaged Navier-Stokes model. Population balance model is implemented in order to describe gas bubble coalescence, breakage and species transport in the reaction medium and to predict oxygen volumetric mass transfer coefficient (kLa). Model results are verified against experimental data and show good agreement as 13 classes of bubble size is taking into account. Flow behavior in different operational conditions is studied. Almost at all impeller speeds and aeration intensities there were acceptable distributions of species caused by proper mixing. The magnitude of dissolved oxygen percentage in aqueous phase has a direct correlation with impeller speed and any increasing of the aeration magnitude leads to faster saturation in shorter periods of time.

  8. Experimental and CFD-PBM approach coupled with a simplified dynamic analysis of mass transfer in phenol biodegradation in a three phase system of an aerated two-phase partitioning bioreactor for environmental applications

    Science.gov (United States)

    Moradkhani, Hamed; Anarjan Kouchehbagh, Navideh; Izadkhah, Mir-Shahabeddin

    2017-03-01

    A three-dimensional transient modeling of a two-phase partitioning bioreactor, combining system hydrodynamics, two simultaneous mass transfer and microorganism growth is modeled using computational fluid dynamics code FLUENT 6.2. The simulation is based on standard "k-ɛ" Reynolds-averaged Navier-Stokes model. Population balance model is implemented in order to describe gas bubble coalescence, breakage and species transport in the reaction medium and to predict oxygen volumetric mass transfer coefficient (kLa). Model results are verified against experimental data and show good agreement as 13 classes of bubble size is taking into account. Flow behavior in different operational conditions is studied. Almost at all impeller speeds and aeration intensities there were acceptable distributions of species caused by proper mixing. The magnitude of dissolved oxygen percentage in aqueous phase has a direct correlation with impeller speed and any increasing of the aeration magnitude leads to faster saturation in shorter periods of time.

  9. Existence and stability of weak solutions for a degenerate parabolic system modelling two-phase flows in porous media

    CERN Document Server

    Escher, Joachim; Matioc, Bogdan-Vasile

    2011-01-01

    We prove global existence of nonnegative weak solutions to a degenerate parabolic system which models the interaction of two thin fluid films in a porous medium. Furthermore, we show that these weak solutions converge at an exponential rate towards flat equilibria.

  10. LITERATURE REVIEW: HEAT TRANSFER THROUGH TWO-PHASE INSULATION SYSTEMS CONSISTING OF POWDERS IN A CONTINUOUS GAS PHASE

    Science.gov (United States)

    The report, a review of the literature on heat flow through powders, was motivated by the use of fine powder systems to produce high thermal resistivities (thermal resistance per unit thickness). he term "superinsulations" has been used to describe this type of material, which ha...

  11. High resolution of racemic phenylalanine with dication imidazolium-based chiral ionic liquids in a solid-liquid two-phase system.

    Science.gov (United States)

    Huang, Xiaoxia; Wu, Haoran; Wang, Zhixia; Luo, Yingjie; Song, Hang

    2017-01-06

    A novel solid-liquid two-phase system was developed for the chiral separation of racemic phenylalanine with new dication imidazolium-based chiral ionic liquids. Preliminary experiments showed distinct enantioselectivity in amino acid extraction with the novel solid-liquid two-phase system, more L-enantiomer of amino acid cooperatively interacted with ionic liquids and copper ions to be the solid phase. Various factors, including the alkyl chain length of cations of ionic liquids, the amount of copper acetate, the ratio of n(ILs)/n(Cu(2+)), the amount of water and racemic phenylalanine, the resolution time together with the resolution temperature, were systematically investigated for their influence on resolution efficiency. The results showed that, under a certain condition, the enantiomeric excess value and the yield of phenylalanine in liquid phase (mainly containing D-enantiomer) were 67.8% and 96.5%, the enantiomeric excess value and the yield of phenylalanine in solid phase (mainly containing L-enantiomer) were 99.2% and 85.2%. Finally, 2D NMR technology, infrared spectroscopy and molecular simulation method were used to study the interaction mechanism. The results indicated that L-enantiomer of phenylalanine interacts more strongly with chiral ILs and Cu(2+). The novel system has characteristics of free-organic solvent, simple operation, fast separation process and very high resolution efficiency for racemic phenylalanine. This work could provide a new and alternative resolution approach for other chiral separations.

  12. Photochemical oxidation of thiophene by O2 in an oil/acetonitrile two-phase extraction system.

    Science.gov (United States)

    Li, Fa-Tang; Zhao, Di-Shun; Li, Hong-Xia; Liu, Rui-Hong

    2008-10-01

    Photochemical oxidation of thiophene in an n-octane/acetonitrile extraction system using O(2) as oxidant was studied. Results obtained here can be used as a reference for desulfurization of gasoline, because thiophene is one of the main components containing sulfur in fluid catalytic cracking gasoline. A 500-W high-pressure mercury lamp was used as a light source for irradiation, and air was introduced by a gas pump to supply O(2). Thiophene dissolved in nopolar n-octane solvent was photodecomposed and removed into the polar acetonitrile phase. The desulfurization rate of thiophene in n-octane was 65.2% under photoirradiation for 5 h under the conditions of air flow at 150 mL min(-1), and V(n-octane):V(acetonitrile) = 1:1. This can be improved to 96.5% by adding 0.15 g Na-ZSM-5 zeolite into the 100-mL reaction system, which is the absorbent for O(2) and thiophene. Under such conditions, the photooxidation kinetics of thiophene with O(2) and Na-ZSM-5 zeolite is first-order with an apparent rate constant of 0.6297 h(-1) and half-time of 1.10 h. The sulfur content can be reduced from 800 microL L(-1) to 28 microL L(-1).

  13. Microstructure Formations in the Two-Phase Region of the Binary Peritectic Organic System TRIS-NPG

    Science.gov (United States)

    Mogeritsch, Johann; Ludwig, Andreas

    2012-01-01

    In order to prepare for an onboard experiment on the International Space Station (ISS), systematic directional solidification experiments with transparent hypoperitectic alloys were carried out at different solidification rates around the critical velocity for morphological stability of both solid phases. The investigations were done in the peritectic region of the binary transparent organic TRIS-NPG system where the formation of layered structures is expected to occur. The transparent appearance of the liquid and solid phase enables real time observations of the dynamic of pattern formation during solidification. The investigations show that frequently occurring nucleation events govern the peritectic solidification morphology which occurs at the limit of morphological stability. As a consequence, banded structures lead to coupled growth even if the lateral growth is much faster compared to the growth in pulling direction.

  14. Study and Implementation of a Two-Phase Interleaved Bidirectional DC/DC Converter for Vehicle and DC-Microgrid Systems

    Directory of Open Access Journals (Sweden)

    Ching-Ming Lai

    2015-09-01

    Full Text Available The objective of this paper is to implement a two-phase, interleaved, bidirectional DC/DC converter topology with an improved voltage conversion ratio for electric vehicle (EV and DC-microgrid systems. In this study, a two-phase interleaved charge-pump topology is introduced to achieve a high voltage conversion ratio with very simple control circuits. In discharge mode, the circuit topology acts as a voltage-multiplier boost converter to achieve a high step-up conversion ratio (48 V to 240 V. In charge mode, the circuit topology acts as a voltage-divider buck converter to achieve a high voltage step-down conversion ratio (240 V to 48 V. The circuit configuration, operating principle, steady-state analysis and the closed-loop control of the proposed converter are presented. Experiments conducted on a laboratory prototype with 500 W power-rating are presented to verify the effectiveness. The maximum efficiency levels in discharge and charge modes are about 97.7% and 98.4% respectively.

  15. Corrosion inhibitor for aqueous ammonia absorption system

    Science.gov (United States)

    Phillips, Benjamin A.; Whitlow, Eugene P.

    1998-09-22

    A method of inhibiting corrosion and the formation of hydrogen and thus improving absorption in an ammonia/water absorption refrigeration, air conditioning or heat pump system by maintaining the hydroxyl ion concentration of the aqueous ammonia working fluid within a selected range under anaerobic conditions at temperatures up to 425.degree. F. This hydroxyl ion concentration is maintained by introducing to the aqueous ammonia working fluid an inhibitor in an amount effective to produce a hydroxyl ion concentration corresponding to a normality of the inhibitor relative to the water content ranging from about 0.015 N to about 0.2 N at 25.degree. C. Also, working fluids for inhibiting the corrosion of carbon steel and resulting hydrogen formation and improving absorption in an ammonia/water absorption system under anaerobic conditions at up to 425.degree. F. The working fluids may be aqueous solutions of ammonia and a strong base or aqueous solutions of ammonia, a strong base, and a specified buffer.

  16. Sulphonamide porphyrins in the biomimetic oxidation by H{sub 2}O{sub 2}. An efficient two-phase system

    Energy Technology Data Exchange (ETDEWEB)

    Rocha Gonsalves, A.M. d`A.; Pereira, M.M.; Serra, A.C. [Departamento de Quimica: Universidade de Coimbra. Coimbra (Portugal)

    1996-12-31

    A catalytic biphasic aqueous/organic system for hydrogen peroxide oxidations was studied and improved, and a new metalloporphyrins derived from meso-tetrakis-2,6-dichlorophenylprophyr in bearing five sulphonamide side chains in the meso-phenyl and in one of the Beta-positions was synthesised and established as an highly efficient and stable catalyst working in the newly developed system. An interface mechanism is proposed to interpret the role of lipophilic acids as co-catalysts, the efficiency of the catalysis and the stability of the catalyst in the new system. (Author) 16 refs.

  17. Extraction of Total Alkaloids from Pomegranate Peel Using Ultrasonic Coupled with Aqueous Two-phase Separation%超声集成双水相体系提取石榴皮中总生物碱的研究

    Institute of Scientific and Technical Information of China (English)

    吴佳; 解成喜

    2012-01-01

    The total alkaloids were extracted from Pomegranate Peel and determined with acid dye colorimtric method. By orthogonal test, the highest extraction yield was obtained when the amount of ammonium sulfate was 8. 0 g,the volume propanol was 20 mL,the alcohol/water ratio of the two-phase extraction system was 0. 8 and ultrasonic extraction time was 60 min. Under the optimized conditions,the extraction rate was 0. 559 mg/g with an average recovery of'100. &%.%研究了石榴皮中总生物碱的提取工艺并对其含量进行测定.以酸性染料比色法为分析手段,通过正交试验,探讨了影响总生物碱提取率因素的范围和最佳组合.石榴皮中总生物碱的最佳提取工艺条件为:硫酸铵8.0g、丙醇用量20 mL、醇水比为0.8∶1的双水相体系、超声时间60 min.在此条件下,石榴皮中总生物碱的平均提取率为0.559 mg/g,平均回收率为100.8%.

  18. Liquid / liquid biphasic electrochemistry in ultra-turrax dispersed acetonitrile / aqueous electrolyte systems

    Energy Technology Data Exchange (ETDEWEB)

    Watkins, John D. [Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY (United Kingdom); Amemiya, Fumihiro; Atobe, Mahito [Tokyo Institute of Technology, Department of Electronic Chemistry, Yokohama, Kanagawa 2268502 (Japan); Bulman-Page, Philip C. [School of Chemistry, University of East Anglia, Norwich, Norfolk NR4 7TJ (United Kingdom); Marken, Frank, E-mail: F.Marken@bath.ac.u [Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY (United Kingdom)

    2010-12-01

    Unstable acetonitrile | aqueous emulsions generated in situ with ultra-turrax agitation are investigated for applications in dual-phase electrochemistry. Three modes of operation for liquid / liquid aqueous-organic electrochemical processes are demonstrated with no intentionally added electrolyte in the organic phase based on (i) the formation of a water-soluble product in the aqueous phase in the presence of the organic phase, (ii) the formation of a product and ion transfer at the liquid / liquid-electrode triple phase boundary, and (iii) the formation of a water-insoluble product in the aqueous phase which then transfers into the organic phase. A three-electrode electrolysis cell with ultra-turrax agitator is employed and characterised for acetonitrile / aqueous 2 M NaCl two phase electrolyte. Three redox systems are employed in order to quantify the electrolysis cell performance. The one-electron reduction of Ru(NH{sub 3}){sub 6}{sup 3+} in the aqueous phase is employed to determine the rate of mass transport towards the electrode surface and the effect of the presence of the acetonitrile phase. The one-electron oxidation of n-butylferrocene in acetonitrile is employed to study triple phase boundary processes. Finally, the one-electron reduction of cobalticenium cations in the aqueous phase is employed to demonstrate the product transfer from the electrode surface into the organic phase. Potential applications in biphasic electrosynthesis are discussed.

  19. Validation and comparison of two-phase flow modeling capabilities of CFD, sub channel and system codes by means of post-test calculations of BFBT transient tests

    Energy Technology Data Exchange (ETDEWEB)

    Jaeger, Wadim; Manes, Jorge Perez; Imke, Uwe; Escalante, Javier Jimenez; Espinoza, Victor Sanchez, E-mail: victor.sanchez@kit.edu

    2013-10-15

    Highlights: • Simulation of BFBT turbine and pump transients at multiple scales. • CFD, sub-channel and system codes are used for the comparative study. • Heat transfer models are compared to identify difference between the code predictions. • All three scales predict results in good agreement to experiment. • Sub cooled boiling models are identified as field for future research. -- Abstract: The Institute for Neutron Physics and Reactor Technology (INR) at the Karlsruhe Institute of Technology (KIT) is involved in the validation and qualification of modern thermo hydraulic simulations tools at various scales. In the present paper, the prediction capabilities of four codes from three different scales – NEPTUNE{sub C}FD as fine mesh computational fluid dynamics code, SUBCHANFLOW and COBRA-TF as sub channels codes and TRACE as system code – are assessed with respect to their two-phase flow modeling capabilities. The subject of the investigations is the well-known and widely used data base provided within the NUPEC BFBT benchmark related to BWRs. Void fraction measurements simulating a turbine and a re-circulation pump trip are provided at several axial levels of the bundle. The prediction capabilities of the codes for transient conditions with various combinations of boundary conditions are validated by comparing the code predictions with the experimental data. In addition, the physical models of the different codes are described and compared to each other in order to explain the different results and to identify areas for further improvements.

  20. Pressure Loss across Tube Bundles in Two-phase Flow

    Energy Technology Data Exchange (ETDEWEB)

    Sim, Woo Gun; Banzragch, Dagdan [Hannam Univ., Daejon (Korea, Republic of)

    2016-03-15

    An analytical model was developed by Sim to estimate the two-phase damping ratio for upward two-phase flow perpendicular to horizontal tube bundles. The parameters of two-phase flow, such as void fraction and pressure loss evaluated in the model, were calculated based on existing experimental formulations. However, it is necessary to implement a few improvements in the formulations for the case of tube bundles. For the purpose of the improved formulation, we need more information about the two-phase parameters, which can be found through experimental test. An experiment is performed with a typical normal square array of cylinders subjected to the two-phase flow of air-water in the tube bundles, to calculate the two-phase Euler number and the two-phase friction multiplier. The pitch-to-diameter ratio is 1.35 and the diameter of cylinder is 18mm. Pressure loss along the flow direction in the tube bundles is measured with a pressure transducer and data acquisition system to calculate the two-phase Euler number and the two-phase friction multiplier. The void fraction model by Feenstra et al. is used to estimate the void fraction of the two-phase flow in tube bundles. The experimental results of the two phase friction multiplier and two-phase Euler number for homogeneous and non-homogeneous two-phase flows are compared and evaluated against the analytical results given by Sim's model.

  1. Supramolecular Complexes Formed by the Self-assembly of Hydrophobic Bis(Zn(2+)-cyclen) Complexes, Copper, and Di- or Triimide Units for the Hydrolysis of Phosphate Mono- and Diesters in Two-Phase Solvent Systems (Cyclen=1,4,7,10-Tetraazacyclododecane).

    Science.gov (United States)

    Hisamatsu, Yosuke; Miyazawa, Yuya; Yoneda, Kakeru; Miyauchi, Miki; Zulkefeli, Mohd; Aoki, Shin

    2016-01-01

    We previously reported on supramolecular complexes 4 and 5, formed by the 4 : 4 : 4 or 2 : 2 : 2 assembly of a dimeric zinc(II) complex (Zn2L(1)) having 2,2'-bipyridyl linker, dianion of cyanuric acid (CA) or 5,5-diethylbarbituric acid (Bar), and copper(II) ion (Cu(2+)) in an aqueous solution. The supermolecule 4 possesses Cu2(μ-OH)2 centers and catalyzes hydrolysis of phosphate monoester dianion, mono(4-nitrophenyl)phosphate (MNP), at neutral pH. In this manuscript, we report on design and synthesis of hydrophobic supermolecules 9 and 10 by 4 : 4 : 4 and 2 : 2 : 2 self-assembly of hydrophobic Zn2L(2) and Zn2L(3) containing long alkyl chains, CA or Bar, and Cu(2+) and their phosphatase activity for the hydrolysis of MNP and bis(4-nitrophenyl)phosphate (BNP) in two-phase solvent systems. We assumed that the Cu2(μ-OH)2 active sites of 9 and 10 would be more stable in organic solvent than in aqueous solution and that product inhibition of the supermolecules might be avoided by the release of HPO4(2-) into the aqueous layer. The findings indicate that 9 and 10 exhibit phosphatase activity in the two-phase solvent system, although catalytic turnover was not observed. Furthermore, the hydrolysis of BNP catalyzed by the hydrophobic 2 : 2 : 2 supermolecules in the two-phase solvent system is described.

  2. Enhanced thermionic emission properties in textured two-phase LaB{sub 6}–BaB{sub 6} system prepared by spark plasma sintering

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, Shen Lin, E-mail: shenlinz@sina.com [College of Mathematics and Physics, Jinggangshan University, Jian 343409 (China); Shanghai Key Laboratory of Special Artificial Microstructure and Pohl Institute of Solid State Physics and School of Physics Science and Engineering, Tongji University, Shanghai 200092 (China); State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 100190 (China); Zhang, Jiu Xing, E-mail: zjiuxing@hfut.edu.cn [School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009 (China); Bao, Li Hong [Inner Mongolia Key Laboratory for Physics and Chemistry of Functional Materials, Inner Mongolia Normal University, Hohhot 010022 (China); Yu, Xiao Guang; Hu, Qiang Lin; Hu, Dong Qiang [College of Mathematics and Physics, Jinggangshan University, Jian 343409 (China)

    2014-10-25

    Graphical abstract: Room temperature XRD patterns of the nominal composition (La{sub 0.6}Ba{sub 0.4})B{sub 6} synthesized by SPS at 1400 °C for 5 min under 50 MPa. The XRD patterns reveal that the (La{sub 0.6}Ba{sub 0.4})B{sub 6} is a two-phase mixture of LaB{sub 6} and BaB{sub 6}. The inset is a macroscopic picture of the obtained (La{sub 0.6}Ba{sub 0.4})B{sub 6}. - Highlights: • (La{sub 0.6}Ba{sub 0.4})B{sub 6} has been successfully prepared by spark plasma sintering. • The obtained (La{sub 0.6}Ba{sub 0.4})B{sub 6} compound is a two-phase mixture of LaB{sub 6} and BaB{sub 6}. • The (0 0 1) texture is favorable for the decrease of work function. • The addition of BaB{sub 6} to LaB{sub 6} improves the electron emission performance. • (La{sub 0.6}Ba{sub 0.4})B{sub 6} is shown to be promising candidate for cathode applications. - Abstract: Structure, crystallographic orientation, and thermionic emission properties of the two-phase LaB{sub 6}–BaB{sub 6} system fabricated by a combined process of evaporation–condensation and spark plasma sintering (SPS) were investigated systematically. The powder X-ray diffraction (XRD) reveals that the obtained nominal composition (La{sub 0.6}Ba{sub 0.4})B{sub 6} compound exhibits coexistence of two isostructural phases of LaB{sub 6} and BaB{sub 6}. The electron backscatter diffraction (EBSD) confirms that the surface of the (La{sub 0.6}Ba{sub 0.4})B{sub 6} forms a clear (0 0 1) texture, which favors reduction in work function. Thermionic emission properties were measured in the temperature range between 1500 and 1873 K. The results indicated that the (La{sub 0.6}Ba{sub 0.4})B{sub 6} emitter displayed a low work function of 1.95 eV, and a high zero field emission current density of 10 A cm{sup −2} at 1773 K. Compared to the reported LaB{sub 6} and (La{sub 0.6}Sm{sub 0.4})B{sub 6} prepared for identical synthesis conditions, (La{sub 0.6}Ba{sub 0.4})B{sub 6} is shown to have the most excellent thermionic emission

  3. Reconstruction of electrical capacitance tomography images based on fast linearized alternating direction method of multipliers for two-phase flow system

    Institute of Scientific and Technical Information of China (English)

    Chongkun Xia; Chengli Su⁎; Jiangtao Cao; Ping Li

    2016-01-01

    Electrical capacitance tomography (ECT) has been applied to two-phase flow measurement in recent years. Image reconstruction algorithms play an important role in the successful applications of ECT. To solve the il-posed and nonlinear inverse problem of ECT image reconstruction, a new ECT image reconstruction method based on fast lin-earized alternating direction method of multipliers (FLADMM) is proposed in this paper. On the basis of theoretical analysis of compressed sensing (CS), the data acquisition of ECT is regarded as a linear measurement process of permittivity distribution signal of pipe section. A new measurement matrix is designed and L1 regularization method is used to convert ECT inverse problem to a convex relaxation problem which contains prior knowledge. A new fast alternating direction method of multipliers which contained linearized idea is employed to minimize the objective function. Simulation data and experimental results indicate that compared with other methods, the quality and speed of reconstructed images are markedly improved. Also, the dynamic experimental results in-dicate that the proposed algorithm can fulfil the real-time requirement of ECT systems in the application.

  4. Extração no ponto nuvem de compostos orgânicos homólogos e isômeros Extraction of homologous and isomeric organic compounds by two-phase aqueous extraction

    Directory of Open Access Journals (Sweden)

    Lindemberg J. N. Duarte

    2005-06-01

    Full Text Available In this work, the feasibility of employing micelle-mediated extraction for selective separation of homologous or isomeric organic compounds is demonstrated. Firstly, the main parameters controlling extraction performances, such as surfactant concentration and temperature were varied. A Scheffé-type experimental design was demonstrated as a novel and useful method to characterize the various experimental factors. At each point selected in the two-phase domain and for a given solute, extraction percentage (E%, concentration ratio, phase volume ratio, and equilibrium partition coefficient (K C were determined. The values of E% and K C decrease in the following order: phenol > 1-phenylethanol ~ 2-phenylethanol > benzyl alcohol.

  5. Coupling two-phase fluid flow with two-phase darcy flow in anisotropic porous media

    KAUST Repository

    Chen, J.

    2014-06-03

    This paper reports a numerical study of coupling two-phase fluid flow in a free fluid region with two-phase Darcy flow in a homogeneous and anisotropic porous medium region. The model consists of coupled Cahn-Hilliard and Navier-Stokes equations in the free fluid region and the two-phase Darcy law in the anisotropic porous medium region. A Robin-Robin domain decomposition method is used for the coupled Navier-Stokes and Darcy system with the generalized Beavers-Joseph-Saffman condition on the interface between the free flow and the porous media regions. Obtained results have shown the anisotropic properties effect on the velocity and pressure of the two-phase flow. 2014 Jie Chen et al.

  6. Extraction of Phenylalanine Phase Systems Containing Enantiomers by Aqueous Two Combinatorial Chiral Selector

    Institute of Scientific and Technical Information of China (English)

    陈晓青; 刘莉; 焦飞鹏; 王珍

    2012-01-01

    In order to obtain a better enantioselectivity of phenylalanine enantiomers and establish the optimal chiral ex- traction conditions, the distribution behavior was investigated in aqueous two-phase systems which were composed of polyethylene glycol and ammonium sulfate containing combinatorial chiral selector: β-cyclodextrin and HP-β-cyclodextrin. The influence of the molar concentration ratio of combinatorial chiral selectors, the total molar concentration of combinatorial chiral selectors, pH value, buffer type and its concentration were thoroughly studied, respectively. The results show that the enantioselectivity reaches 1.53 under the optimal chiral extraction conditions This extraction is a potential economical and effective way for chiral resolution.

  7. Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli

    OpenAIRE

    Gallego-Jara, Julia; de Diego, Teresa; del Real, Álvaro; Écija-Conesa, Ana; Manjón, Arturo; Cánovas, Manuel

    2015-01-01

    Lycopene is an import ant compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic solvents was found to maximize lycopene production through in situ extraction from cells. Within the reactor, three phases were formed during the process: an aqueous phase containing the recombinant...

  8. Contrasted Fitness Costs of Docking and Antibacterial Constructs in the EE and EVida3 Strains Validates Two-Phase Anopheles gambiae Genetic Transformation System.

    Directory of Open Access Journals (Sweden)

    Doug Paton

    Full Text Available The deployment of transgenic mosquitoes carrying genes for refractoriness to malaria has long been seen as a futuristic scenario riddled with technical difficulties. The integration of anti-malarial effector genes and a gene-drive system into the mosquito genome without affecting mosquito fitness is recognized as critical to the success of this malaria control strategy. Here we conducted detailed fitness studies of two Anopheles gambiae s.s. transgenic lines recently developed using a two-phase targeted genetic transformation system. In replicated cage-invasion experiments, males and females of the EE Phase-1 docking strain and EVida3 Phase-2 strain loaded with an antimicrobial peptide (AMP expressed upon blood-feeding, were mixed with individuals of a recently-colonized strain of the Mopti chromosomal form. The experimental design enabled us to detect initial strain reproductive success differences, assortative mating and hybrid vigor that may characterize mosquito release situations. In addition, the potential fitness costs of the unloaded Phase-1 and loaded Phase-2 genetic constructs, independent of the strains' original genetic backgrounds, were estimated between the 1(st instar larvae, pupae and adult stages over 10 generations. The Phase-1 unloaded docking cassette was found to have significantly lower allelic fitness relative to the wild type allele during larval development. However, overall genotypic fitness was comparable to the wild type allele across all stages leading to stable equilibrium in all replicates. In contrast, the Phase-2 construct expressing EVida3 disappeared from all replicates within 10 generations due to lower fitness of hemi- and homozygous larvae, suggesting costly background AMP expression and/or of the DsRed2 marker. This is the first study to effectively partition independent fitness stage-specific determinants in unloaded and loaded transgenic strains of a Phase-1-2 transformation system. Critically, the high

  9. Contrasted Fitness Costs of Docking and Antibacterial Constructs in the EE and EVida3 Strains Validates Two-Phase Anopheles gambiae Genetic Transformation System.

    Science.gov (United States)

    Paton, Doug; Underhill, Anne; Meredith, Janet; Eggleston, Paul; Tripet, Frederic

    2013-01-01

    The deployment of transgenic mosquitoes carrying genes for refractoriness to malaria has long been seen as a futuristic scenario riddled with technical difficulties. The integration of anti-malarial effector genes and a gene-drive system into the mosquito genome without affecting mosquito fitness is recognized as critical to the success of this malaria control strategy. Here we conducted detailed fitness studies of two Anopheles gambiae s.s. transgenic lines recently developed using a two-phase targeted genetic transformation system. In replicated cage-invasion experiments, males and females of the EE Phase-1 docking strain and EVida3 Phase-2 strain loaded with an antimicrobial peptide (AMP) expressed upon blood-feeding, were mixed with individuals of a recently-colonized strain of the Mopti chromosomal form. The experimental design enabled us to detect initial strain reproductive success differences, assortative mating and hybrid vigor that may characterize mosquito release situations. In addition, the potential fitness costs of the unloaded Phase-1 and loaded Phase-2 genetic constructs, independent of the strains' original genetic backgrounds, were estimated between the 1(st) instar larvae, pupae and adult stages over 10 generations. The Phase-1 unloaded docking cassette was found to have significantly lower allelic fitness relative to the wild type allele during larval development. However, overall genotypic fitness was comparable to the wild type allele across all stages leading to stable equilibrium in all replicates. In contrast, the Phase-2 construct expressing EVida3 disappeared from all replicates within 10 generations due to lower fitness of hemi- and homozygous larvae, suggesting costly background AMP expression and/or of the DsRed2 marker. This is the first study to effectively partition independent fitness stage-specific determinants in unloaded and loaded transgenic strains of a Phase-1-2 transformation system. Critically, the high fitness of the

  10. Advanced Aqueous Separation Systems for Actinide Partitioning

    Energy Technology Data Exchange (ETDEWEB)

    Nash, Ken [Washington State Univ., Pullman, WA (United States); Martin, Leigh [Idaho National Lab. (INL), Idaho Falls, ID (United States); Lumetta, Gregg [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-04-02

    One of the most challenging aspects of advanced processing of used nuclear fuel is the separation of transplutonium actinides from fission product lanthanides. This separation is essential if actinide transmutation options are to be pursued in advanced fuel cycles, as lanthanides compete with actinides for neutrons in both thermal and fast reactors, thus limiting efficiency. The separation is difficult because the chemistry of Am3+ and Cm3+ is nearly identical to that of the trivalent lanthanides (Ln3+). The prior literature teaches that two approaches offer the greatest probability of devising a successful group separation process based on aqueous processes: 1) the application of complexing agents containing ligand donor atoms that are softer than oxygen (N, S, Cl-) or 2) changing the oxidation state of Am to the IV, V, or VI state to increase the essential differences between Am and lanthanide chemistry (an approach utilized in the PUREX process to selectively remove Pu4+ and UO22+ from fission products). The latter approach offers the additional benefit of enabling a separation of Am from Cm, as Cm(III) is resistant to oxidation and so can easily be made to follow the lanthanides. The fundamental limitations of these approaches are that 1) the soft(er) donor atoms that interact more strongly with actinide cations than lanthanides form substantially weaker bonds than oxygen atoms, thus necessitating modification of extraction conditions for adequate phase transfer efficiency, 2) soft donor reagents have been seen to suffer slow phase transfer kinetics and hydro-/radiolytic stability limitations and 3) the upper oxidation states of Am are all moderately strong oxidants, hence of only transient stability in media representative of conventional aqueous separations systems. There are examples in the literature of both approaches having been described. However, it is not clear at present that any extant process is sufficiently robust for application at the scale

  11. McClellan Air Force Base operable unit B, two-phase extraction system demonstration test, work implementation plan for McClellan AFB, California. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-03

    This document is an integrated demonstration and work plan that presents the technical approach for design, implementation, and testing of two-phase extraction as compared with pump and treat technology in Operable Unit B, investigative cluster IC1 at the McClellan Air Force Base. This work is being coordinated with Clean Sites under a cooperative agreement with EPA's Technology Innovation Office and Superfund Innovative Technology Evaluation Program.

  12. Pumped two-phase heat transfer loop

    Science.gov (United States)

    Edelstein, Fred

    1988-01-01

    A pumped loop two-phase heat transfer system, operating at a nearly constant temperature throughout, includes several independently operating grooved capillary heat exchanger plates supplied with working fluid through independent flow modulation valves connected to a liquid supply line, a vapor line for collecting vapor from the heat exchangers, a condenser between the vapor and the liquid lines, and a fluid circulating pump between the condenser and the heat exchangers.

  13. Two-phase viscoelastic jetting

    Energy Technology Data Exchange (ETDEWEB)

    Yu, J-D; Sakai, S.; Sethian, J.A.

    2008-12-10

    A coupled finite difference algorithm on rectangular grids is developed for viscoelastic ink ejection simulations. The ink is modeled by the Oldroyd-B viscoelastic fluid model. The coupled algorithm seamlessly incorporates several things: (1) a coupled level set-projection method for incompressible immiscible two-phase fluid flows; (2) a higher-order Godunov type algorithm for the convection terms in the momentum and level set equations; (3) a simple first-order upwind algorithm for the convection term in the viscoelastic stress equations; (4) central difference approximations for viscosity, surface tension, and upper-convected derivative terms; and (5) an equivalent circuit model to calculate the inflow pressure (or flow rate) from dynamic voltage.

  14. Review of two-phase instabilities

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Han Ok; Seo, Han Ok; Kang, Hyung Suk; Cho, Bong Hyun; Lee, Doo Jeong

    1997-06-01

    KAERI is carrying out a development of the design for a new type of integral reactors. The once-through helical steam generator is important design features. The study on designs and operating conditions which prevent flow instability should precede the introduction of one-through steam generator. Experiments are currently scheduled to understand two-phase instability, evaluate the effect of each design parameter on the critical point, and determine proper inlet throttling for the prevention of instability. This report covers general two-phase instability with review of existing studies on this topics. The general classification of two phase flow instability and the characteristics of each type of instability are first described. Special attention is paid to BWR core flow instability and once-through steam generator instability. The reactivity feedback and the effect of system parameters are treated mainly for BWR. With relation to once-through steam generators, the characteristics of convective heating and dryout point oscillation are first investigated and then the existing experimental studies are summarized. Finally chapter summarized the proposed correlations for instability boundary conditions. (author). 231 refs., 5 tabs., 47 figs

  15. The influence of secondary flow in a two-phase gas-solid system in straight channels with a non-circular cross-section

    Directory of Open Access Journals (Sweden)

    Milanović Saša M.

    2016-01-01

    Full Text Available The paper considers two-phase gas-solid turbulent flow of pneumatic transport in straight horizontal channels with a non-circular cross-section. During turbulent flow, a specific flow phenomenon, known as secondary flow, occurs in these channels in the cross-sectional plane. The existence of strong temperature gradients in the cross-sectional plane of the channel or the cases of curved channels result in the appearance of the secondary flow of the first kind. However, in straight channels with a non-circular cross-section, in the developed turbulent flow mode, a secondary flow, known as Prandtl’s secondary flow of the second kind, is induced. The paper presents a numerical simulation of a developed two-phase turbulent flow by using the PHOENICS 3.3.1 software package. Reynolds stress model was used to model the turbulence. The paper provides the data on the changes in turbulent stresses in the channel cross-section as well as the velocities of solid particles transported along the channel.

  16. Two-Phase Technology at NASA/Johnson Space Center

    Science.gov (United States)

    Ungar, Eugene K.; Nicholson, Leonard S. (Technical Monitor)

    1999-01-01

    Since the baseline International Space Station (ISS) External Active Thermal Control System (EATCS) was changed from a two-phase mechanically pumped system to a single phase cascade system in the fall of 1993, two-phase EATCS research has continued at a low level at JSC. One of-the lessons of the ISS EATCS selection was that two-phase thermal control systems must have significantly lower power than comparable single phase systems to overcome their larger radiator area, larger line and fluid mass, and perceived higher technical risk. Therefore, research at JSC has concentrated on low power mechanically pumped two-phase EATCSs. In the presentation, the results of a study investigating the trade of single and two-phase mechanically pumped EATCSs for space vehicles will be summarized. The low power two-phase mechanically pumped EATCS system under development at JSC will be described in detail and the current design status of the subscale test unit will be reviewed. Also, performance predictions for a full size EATCS will be presented. In addition to the discussion of two-phase mechanically pumped EATCS development at JSC, two-phase technologies under development for biological water processing will be discussed. These biological water processor technologies are being prepared for a 2001 flight experiment and subsequent usage on the TransHab module on the International Space Station.

  17. Mass transfer coefficient of slug flow for organic solvent-aqueous system in a microreactor

    Energy Technology Data Exchange (ETDEWEB)

    Tuek, Ana Jurinjak; Anic, Iva; Kurtanjek, Zelimir; Zelic, Bruno [University of Zagreb, Zagreb (Croatia)

    2015-06-15

    Application of microreactor systems could be the next break-through in the intensification of chemical and biochemical processes. The common flow regime for organic solvent-aqueous phase two-phase systems is a segmented flow. Internal circulations in segments cause high mass transfer and conversion. We analyzed slug flow in seven systems of organic solvents and aqueous phase. To analyze how slug lengths in tested systems depend on linear velocity and physical and chemical properties of used organic solvents, regression models were proposed. It was shown that models based on linearization of approximation by potentials give low correlation for slug length prediction; however, application of an essential nonlinear model of multiple layer perception (MLP) neural network gives high correlation with R{sup 2}=0.9. General sensitivity analysis was applied for the MLP neural network model, which showed that 80% of variance in slug length for the both phases is accounted for the viscosity and density of the organic phases; 10% is accounted by surface tension of the organic phase, while molecular masses and flow rates each account for 5%. For defined geometry of microreactor, mass transfer has been determined by carrying out the neutralization experiment with NaOH where acetic acid diffuses from organic phase (hexane) into aqueous phase. Estimated mass transfer coefficients were in the range k{sub L}a=4,652-1,9807 h{sup -1}.

  18. Two phase decision algorithm of replica allocation

    Institute of Scientific and Technical Information of China (English)

    Zuo Chaoshu; Liu Xinsong; Wang Zheng; Li Yi

    2006-01-01

    In distributed parallel server system, location and redundancy of replicas have great influence on availability and efficiency of the system. In order to improve availahility and efficiency of the system, two phase decision algorithm of replica allocation is proposed. The algorithm which makes use of auto-regression model dynamically predicts the future count of READ and WRITE operation, and then determines location and redundancy of replicas by considering availability, CPU and bands of the network. The algorithm can not only ensure the requirement of availability, but also reduce the system resources consumed by all the operations in a great scale. Analysis and test show that communication complexity and time complexity of the algorithm satisfy O( n ), resource optimizing scale increases with the increase of READ count.

  19. Performance and microbial community dynamics in a two-phase anaerobic co-digestion system using cassava dregs and pig manure.

    Science.gov (United States)

    Ren, Jiwei; Yuan, Xufeng; Li, Jie; Ma, Xuguang; Zhao, Ye; Zhu, Wanbing; Wang, Xiaofen; Cui, Zongjun

    2014-03-01

    The two-phase anaerobic co-digestion of cassava dregs (CD) with pig manure (PM) was evaluated using four sequencing batch reactors (SBRs) and a continuously stirred tank reactor (CSTR). The effect of seven different PM to CD volatile solid ratios (10:0, 8:2, 6:4, 5:5, 4:6, 2:8 and 0:10) on the acidification phase was investigated. Results indicated the concentrations of soluble chemical oxygen demand, NH4-N and volatile fatty acids increased substantially at seven ratios. Co-acidification of PM and CD performed well. Methanogenic fermentation of the acidification products at seven ratios was steady in CSTR. The highest methane yield and VS removal of 0.352m(3)/kg VSadded and 68.5% were achieved at PM:CD (4:6). The microbial population in CSTR was analyzed using molecular methods. Findings revealed that bacteria such as Firmicutes and Bacteroidetes, archaea such as Methanobacteriales and Methanomicrobiales were advantageous populations. Co-digestion of PM and CD supported higher quantity and diversity of methanogens.

  20. Thermo-Fluid Dynamics of Two-Phase Flow

    CERN Document Server

    Ishii, Mamrou

    2011-01-01

    "Thermo-fluid Dynamics of Two-Phase Flow, Second Edition" is focused on the fundamental physics of two-phase flow. The authors present the detailed theoretical foundation of multi-phase flow thermo-fluid dynamics as they apply to: Nuclear reactor transient and accident analysis; Energy systems; Power generation systems; Chemical reactors and process systems; Space propulsion; Transport processes. This edition features updates on two-phase flow formulation and constitutive equations and CFD simulation codes such as FLUENT and CFX, new coverage of the lift force model, which is of part

  1. Microgravity Two-Phase Flow Transition

    Science.gov (United States)

    Parang, M.; Chao, D.

    1999-01-01

    Two-phase flows under microgravity condition find a large number of important applications in fluid handling and storage, and spacecraft thermal management. Specifically, under microgravity condition heat transfer between heat exchanger surfaces and fluids depend critically on the distribution and interaction between different fluid phases which are often qualitatively different from the gravity-based systems. Heat transfer and flow analysis in two-phase flows under these conditions require a clear understanding of the flow pattern transition and development of appropriate dimensionless scales for its modeling and prediction. The physics of this flow is however very complex and remains poorly understood. This has led to various inadequacies in flow and heat transfer modeling and has made prediction of flow transition difficult in engineering design of efficient thermal and flow systems. In the present study the available published data for flow transition under microgravity condition are considered for mapping. The transition from slug to annular flow and from bubbly to slug flow are mapped using dimensionless variable combination developed in a previous study by the authors. The result indicate that the new maps describe the flow transitions reasonably well over the range of the data available. The transition maps are examined and the results are discussed in relation to the presumed balance of forces and flow dynamics. It is suggested that further evaluation of the proposed flow and transition mapping will require a wider range of microgravity data expected to be made available in future studies.

  2. Effects of a two-phase oil-water mouthwash on halitosis.

    Science.gov (United States)

    Yaegaki, K; Sanada, K

    1992-01-01

    Many oral microorganisms possess hydrophobic outer surfaces. A two-phase, oil-water mouthwash has, therefore, recently been developed to remove such oral microorganisms. The oil phase consists of olive oil and other essential oils. The aqueous phase includes cetylpyridinium chloride, which is a disinfectant that promotes the adhesion of microorganisms to oil droplets. This study determined the effects of this mouthwash on the production of volatile sulfide in vivo and in vitro. Neither rinsing with water nor brushing teeth decreased the concentration of sulfide in mouth air at 3.5 h after treatment. A reduction of only 30% of sulfide was observed when a commercial mouthwash was used. However, this study demonstrated that use of the two-phase mouthwash led to approximately 80% reduction of sulfide. Furthermore, volatile sulfide and 2-ketobutyrate productions from methionine in a saliva putrefaction system were completely inhibited by the two-phase mouthwash; and consumption of methionine was decreased by 65 percent. It is concluded that the two-phase mouthwash strongly inhibits the production of volatile sulfide.

  3. Surfactant-coated Candida rugosa Lipase as Catalyst for Hydrolysis of Olive Oil in Solvent-Free Two-Phase System%表面活性剂包衣Candida rugosa脂肪酶在无溶剂下油水两相体系中催化橄榄油水解

    Institute of Scientific and Technical Information of China (English)

    宋宝东; 丁辉; 吴金川; Hayashi Y; Talukder MMR; 王世昌

    2003-01-01

    The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.

  4. Two Phase Technology Development Initiatives

    Science.gov (United States)

    Didion, Jeffrey R.

    1999-01-01

    Three promising thermal technology development initiatives, vapor compression thermal control system, electronics cooling, and electrohydrodynamics applications are outlined herein. These technologies will provide thermal engineers with additional tools to meet the thermal challenges presented by increased power densities and reduced architectural options that will be available in future spacecraft. Goddard Space Flight Center and the University of Maryland are fabricating and testing a 'proto- flight' vapor compression based thermal control system for the Ultra Long Duration Balloon (ULDB) Program. The vapor compression system will be capable of transporting approximately 400 W of heat while providing a temperature lift of 60C. The system is constructed of 'commercial off-the-shelf' hardware that is modified to meet the unique environmental requirements of the ULDB. A demonstration flight is planned for 1999 or early 2000. Goddard Space Flight Center has embarked upon a multi-discipline effort to address a number of design issues regarding spacecraft electronics. The program addressed the high priority design issues concerning the total mass of standard spacecraft electronics enclosures and the impact of design changes on thermal performance. This presentation reviews the pertinent results of the Lightweight Electronics Enclosure Program. Electronics cooling is a growing challenge to thermal engineers due to increasing power densities and spacecraft architecture. The space-flight qualification program and preliminary results of thermal performance tests of copper-water heat pipes are presented. Electrohydrodynamics (EHD) is an emerging technology that uses the secondary forces that result from the application of an electric field to a flowing fluid to enhance heat transfer and manage fluid flow. A brief review of current EHD capabilities regarding heat transfer enhancement of commercial heat exchangers and capillary pumped loops is presented. Goddard Space Flight

  5. Measurement and Correlation of the Phase Diagram Data for 1-Ethyl-3-methylimidazolium Dimethyl phosphate+K3PO4/ K2HPOjK2CO3+ H20 Ionic Liquids Aqueous Two-Phase Systems at T-- 298.15 K%1-乙基-3-甲基咪唑磷酸二甲酯盐+K3P04/K2HP04/K2C03+H20离子液体双水相体系在T=298.15K时相图数据的测量和相关性

    Institute of Scientific and Technical Information of China (English)

    王良; 朱红; 闫永胜

    2012-01-01

    实验测定了1-乙基-3.甲基咪唑磷酸二甲酯盐(1-ethyl-3-methylimidazolium dimehtyl phosphate, [Emim]DMP )+盐(K3P04,K2HP04和K2C03)+H20体系在298.15K的双节点数据.用3个经验方程与双节点数据关联,发现Merchuk方程简便并且准确性好.不同盐的分相能力顺序为K3P04〉K2HP04〉K2C03,这与水合离子Gibbs自由能(△hydG)有关.用Othmer.Tobias方程、Bancroft方程和二元参数方程计算此方法和相应系线数据的可靠性.[Emim]DMP是常见的离子液体,广泛用于萃取石油燃料中的硫.报道T[Emim]DMP+盐+H20体系的相图数据.%Binodal data for the 1-ethyl-3-methylimidazolium dimethyl phosphate ([Emim]DMP)+salt (K3PO4, K2HPO4, and K2CO3)q-H20 systems were experimentally determined at 298.15 K. Three empirical equations were used to correlate binodal data. The Merchuk equation is an uncomplicated one for fine accu racy. The ability of different salts studied for phase separation are in the order of K3PO4 〉 K2HPO4 〉 K2CO3, which may be related to the Gibbs free energy of hydration of the ions (AhydG). The reliability of the calcula tion method and the corresponding tie-line data was described by the Othmer-Tobias equation and Bancroft equation, as well as the twoparameter equation. [Emim]DMP is a familiar ionic liquid, which is extensively used for extractive desulfurization of fuel oils. This is the first time to report the data for phase diagrams of [Emim]DMP 4- salt + water systems.

  6. Critical transport velocity in two-phase, horizontal pipe flow

    Energy Technology Data Exchange (ETDEWEB)

    Sommerville, D. (U.S. Army Chemical Research, Development and Engineering Center, Aberdeen Proving Grounds, MD (US))

    1991-02-01

    This paper reports on the suspension of solid particles or entrainment of liquid droplets in two- phase flow. Theoretical and empirical relationships have been derived for both instances without any consideration to the similarities between the two. However, a general relation for two-phase flow is desirable since there are systems that cannot be readily defined due to the dual (solid/liquid) nature of the transported material, such as colloids, pulp, slurries, and sludge. Using turbulence theory, one general equation can be derived to predict critical transport velocities for two-phase horizontal flow.

  7. Control System of Two-Phase Brushless DC Gyro Motor Based on DSP%基于DSP的两相无刷直流陀螺电机稳速系统

    Institute of Scientific and Technical Information of China (English)

    吴梅锡; 付鲁华; 林玉池

    2013-01-01

    The research of the influence of gyro motor speed stability on the north-finding result is studied in order to improve the gyro north-finding precision. The paper introduces a two-phase brushless DC gyro motor control system based on TMS320LF2407A. First,it expounds the two-phase brushless DC motor's working principle. Then through the design of the hardware and software system, the close-loop control of two-phase brushless DC gyro motor is realized. The experimental results show that,the system can drive the brushless DC gyro motor well starting,braking and smooth running. The precision of the steady speed is less than 10~6 and the characteristics of the system satisfy the gyroscope demand.%为了进一步提高陀螺仪的寻北精度,分析研究了陀螺电机转速大小和转速精度对寻北结果的影响.在此基础上设计了一种基于TMS320LF2407A的两相无刷直流陀螺电机控制系统,阐述了两相无刷直流电机的工作原理,给出了电机的简化结构及数学模型,并通过对硬件系统和软件算法的设计实现了两相无刷直流陀螺电机的闭环控制.实验结果表明:系统可以很好地完成电机的启动、制动和平稳运行,转速精度优于10-6,满足陀螺仪的工作要求.

  8. Aqueous Two-phase System Extraction of Arginine Deiminase%双水相体系萃取精氨酸脱亚胺酶

    Institute of Scientific and Technical Information of China (English)

    李凯; 李成付; 李加友; 刘茜; 焦庆才

    2008-01-01

    报道了利用聚乙二醇/硫酸铵双水相体系从自溶NJ402菌粗提酶液中分离纯化精氨酸脱亚氨酶(ADI)的研究结果,为精氨酸脱亚氨酶的分离纯化提供了一种方法.在双水相体系中采用聚乙二醇(PEG)与(NH4)2SO4为组成成分,考察了聚乙二醇(PEG)平均相对分子质量、PEG质量分数、(NH4)2SO4质量分数、pH及NaCl质量分数对精氨酸脱亚氨酶分离纯化效果的影响.最佳双水相体系萃取条件为:聚乙二醇(PEG)平均相对分子质量为1 000,w(PEG1000)=15%,w[(NH4)2SO4]=20%,pH=6.5,室温下从自溶NJ402菌粗提酶液中分离纯化精氨酸脱亚氨酶,纯化倍数达到2.35倍,萃取率达91.1%.

  9. Two-phase cooling fluids; Les fluides frigoporteurs diphasiques

    Energy Technology Data Exchange (ETDEWEB)

    Lallemand, A. [Institut National des Sciences Appliquees (INSA), 69 - Lyon (France)

    1997-12-31

    In the framework of the diminution of heat transfer fluid consumption, the concept of indirect refrigerating circuits, using cooling intermediate fluids, is reviewed and the fluids that are currently used in these systems are described. Two-phase cooling fluids advantages over single-phase fluids are presented with their thermophysical characteristics: solid fraction, two-phase mixture enthalpy, thermal and rheological properties, determination of heat and mass transfer characteristics, and cold storage through ice slurry

  10. 基于AT89C52单片机的步进电机控制系统研究%Research of Control System of Two-phase Hybrid Stepping Motor Based on AT89C52 Microcontrollers

    Institute of Scientific and Technical Information of China (English)

    何冲; 王淑红; 侯胜伟; 牛慧文

    2012-01-01

    The design scheme Based on AT89C52 Microcontrollers of two phase hybrid stepping motor control system is introduced, reflects two phase hybrid stepping motor control method in principle, increase the flexibility of stepping motor control system design. The control system adopts AT89C52 as controller, use BUW49 power tube of double H bridge type circuit as driver, join button control and LCD1602 display. The circuit structure of control system is simple and the thinking design is clearly. Experimental results shows that the control system operation is simple and reliable, it has strong practicability.%本文介绍了基于AT89C52单片机的两相混合式步进电机控制系统的设计方案,增加了步进电机控制系统设计的灵活性。控制系统采用AT89C52单片机作为控制器,由达林顿功率管BUW49组成的双H桥式电路作为驱动器,加入按键控制及LCD1602显示。控制系统的电路结构简单、设计思路清晰。实验表明,控制系统操作简单、运行可靠,具有较强的实用性。

  11. ORGANIC-HIGH IONIC STRENGTH AQUEOUS SOLVENT SYSTEMS FOR SPIRAL COUNTER-CURRENT CHROMATOGRAPHY: GRAPHIC OPTIMIZATION OF PARTITION COEFFICIENT.

    Science.gov (United States)

    Zeng, Yun; Liu, Gang; Ma, Ying; Chen, Xiaoyuan; Ito, Yoichiro

    2013-01-10

    A new series of organic-high ionic strength aqueous two-phase solvents systems was designed for separation of highly polar compounds by spiral high-speed counter-current chromatography. A total of 21 solvent systems composed of 1-butanol-ethanol-saturated ammonium sulfate-water at various volume ratios are arranged according to an increasing order of polarity. Selection of the two-phase solvent system for a single compound or a multiple sample mixture can be achieved by two steps of partition coefficient measurements using a graphic method. The capability of the method is demonstrated by optimization of partition coefficient for seven highly polar samples including tartrazine (K=0.77), tryptophan (K=1.00), methyl green (K= 0.93), tyrosine (0.81), metanephrine (K=0.89), tyramine (K=0.98), and normetanephrine (K=0.96). Three sulfonic acid components in D&C Green No. 8 were successfully separated by HSCCC using the graphic selection of the two-phase solvent system.

  12. Use of hydrophilic ionic liquids in a two-phase system to improve Mung bean epoxide hydrolases-mediated asymmetric hydrolysis of styrene oxide.

    Science.gov (United States)

    Chen, Wen-Jing; Lou, Wen-Yong; Yu, Chun-Yang; Wu, Hong; Zong, Min-Hua; Smith, Thomas J

    2012-12-31

    A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hydrophilic ILs reduced the amount of non-enzymatic hydrolysis, and improved the reaction rate by up to 22%. The ILs with cation containing an alkanol group, namely [C(2)OHMIM][BF(4)] and [C(2)OHMIM][TfO], and the choline amino acid ILs [Ch][Arg] and [Ch][Pro] were found to be the most suitable co-solvents for the reaction, owing to their good biocompatibility with the enzyme, which led to high initial rates (0.99-1.25 μmol/min) and high product e.e.s (95%). When substrate concentration was around 30 mM, where optimal performance was observed with the IL-containing systems, the product e.e. was improved from 90% without ILs to ≥95% in the presence of ILs.

  13. Two-phases-service queuing system with server breakdown and vacations%带有服务台故障和两阶段服务的休假排队系统

    Institute of Scientific and Technical Information of China (English)

    曾慧; 岳德权

    2011-01-01

    为了解决现实生产生活中的具有两阶段服务性质的休假排队问题,利用马尔可夫过程理论建立系统稳态概率方程组,并利用分块矩阵解法,得到了稳态概率的矩阵解.由此得出系统的平均队长、平均等待队长等性能指标.该成果对解决两阶段服务排队模型具有重要的理论意义和应用价值.%In order to solve the vacation queue problems with a two-phase of service in real-life production, the equations of steady-state probability were derived by applying the Markov process theory in this study. Subsequently, the matrix form solution of steady-state probability was found by using block matrix method. Furthermore, some performance measures of the system, such as the expected number of customers in the system and the expected number of customers in the queue, were also obtained. The study on the queuing systems with two-phase of service is of theoretical significance and application value.

  14. Two-phase flow simulation inside a tubing string with artificial lift system PCP based; Simulacao do escoamento bifasico em uma coluna de producao com sistema de elevacao artificial por BCP

    Energy Technology Data Exchange (ETDEWEB)

    Vidal, F.J.T.; Salazar, A.O.; Maitelli, A.L. [Rio Grande do Norte Univ., Natal, RN (Brazil). Programa de Pos-graduacao em Engenharia Eletrica]. E-mail: francisco@dca.ufrn.br; andres@dca.ufrn.br; maitelli@dca.ufrn.br; Assmann, B.W. [PETROBRAS S.A., Natal/Fortaleza, RN/CE (Brazil). Unidade de Negocios]. E-mail: benno@petrobras.com.br; Lima, J.A. [Rio Grande do Norte Univ., Natal, RN (Brazil). Dept. de Engenharia Mecanica. Programa de Pos-graduacao em Engenharia Mecanica]. E-mail: jalima@dem.ufrn.br

    2005-07-01

    The main goal of the present work is the computational simulation of the vertical two-phase flow within a tubing string in the production of oil equipped with a PCP artificial lift system (Progressive Cavity Pumping). By initially adopting the homogeneous model for the two-phase mixture (oil and gas), the fields of velocity and pressure are evaluated for prediction of pressure loss along the tubing, as well as the spatial and temporal behavior of typical parameters as gas-oil ratio, bubble pressure, solubility ratio, void fraction, gas and oil formation volume factors, among others. Prediction of these properties is an integral part of pressure loss calculations, as well as they constitute essential parameters for optimization of any artificial lift system. The numerical simulation is based on the transport equations (continuity and momentum equations) for a pseudo-fluid through the finite difference method, and the mixture properties are evaluated by employing the black-oil fluid model. Behavior analyses of the main flow variables are made and results for a typical artificial lift system PCP based are discussed. (author)

  15. Efficacy and Safety of Epratuzumab in Moderately to Severely Active Systemic Lupus Erythematosus: Results From Two Phase III Randomized, Double‐Blind, Placebo‐Controlled Trials

    Science.gov (United States)

    Wallace, Daniel J.; Furie, Richard A.; Petri, Michelle A.; Pike, Marilyn C.; Leszczyński, Piotr; Neuwelt, C. Michael; Hobbs, Kathryn; Keiserman, Mauro; Duca, Liliana; Kalunian, Kenneth C.; Galateanu, Catrinel; Bongardt, Sabine; Stach, Christian; Beaudot, Carolyn; Kilgallen, Brian; Gordon, Caroline; Batalov, A.; Bojinca, M.; Djerassi, R.; Duca, L.; Horak, P.; Kolarov, Z.; Milasiene, R.; Monova, D.; Otsa, K.; Pileckyte, M.; Popova, T.; Radulescu, F.; Rashkov, R.; Rednic, S.; Repin, M.; Stoilov, R.; Tegzova, D.; Vezikova, N.; Vitek, P.; Zainea, C.; East, Far; Baek, H.; Chen, Y.; Chiu, Y.; Cho, C.; Chou, C.; Choe, J.; Huang, C.; Kang, Y.; Kang, S.; Lai, N.; Lee, S.; Park, W.; Shim, S.; Suh, C.; Yoo, W.; Armengol, H. Avila; Zapata, F. Avila; Santiago, M. Barreto; Cavalcanti, F.; Chahade, W.; Costallat, L.; Keiserman, M.; Alcala, J. Orozco; Remus, C. Ramos; Roimicher, L.; Abu‐Shakra, M.; Agarwal, V.; Agmon‐Levin, N.; Kadel, J.; Levy, Y.; Mevorach, D.; Paran, D.; Reitblat, T.; Rosner, I.; Shobha, V.; Sthoeger, Z.; Zisman, D.; Ayesu, K.; Berney, S.; Box, J.; Busch, H.; Buyon, J.; Carter, J.; Chi, J.; Clowse, M.; Collins, R.; Dao, K.; Diab, I.; Dikranian, A.; El‐Shahawy, M.; Gaylis, N.; Grossman, J.; Halpert, E.; Huff, J.; Jarjour, W.; Kao, A.; Katz, R.; Kennedy, A.; Khan, M.; Kivitz, A.; Kohen, M.; Lawrence‐Ford, T.; Lawson, J.; Levesque, M.; Lowenstein, M.; Majjhoo, A.; Mcarthur, R.; McLain, D.; Merrill, J.; Murillo, A.; Neucks, S.; Niemer, G.; Noaiseh, G.; Parker, C.; Pantojas, C.; Pattanaik, D.; Petri, M.; Pickrell, P.; Reveille, J.; Roman‐Miranda, A.; Rothfield, N.; Sankoorikal, A.; Sayers, M.; Singhal, A.; Snyder, A.; Striebich, C.; Vo, Q.; von Feldt, J.; Wallace, D.; Wasko, M.; Young, C.; Adelstein, S.; Hall, S.; Littlejohn, G.; Nicholls, D.; Suranyi, M.; Amoura, Z.; Bannert, B.; Behrens, F.; Perez, L.Carreno; Chakravarty, K.; Gonzales, F. Diaz; Davies, K.; Doria, A.; Emery, P.; Fernández‐Nebro, A.; Govoni, M.; Hachulla, E.; Hellmich, B.; Houssiau, F.; Malaise, M.; Margaux, J.; Maugars, Y.; Muñoz‐Fernández, S.; Navarro, F.; Ordi‐Ros, J.; Pellerito, R.; Pena‐Sagredo, J.; Roussou, E.; Schmidt, R. E.; Ucar‐Angulo, E.; Viallard, J‐F.; Westhovens, R.; Worm, M.; Yee, C. S.; Nayiager, S.; Reuter, H.; Spargo, C.; Bazela, B.; Brzosko, M.; Chudzik, D.; Gasztonyi, B.; Geher, P.; Ionescu, R.; Jeka, S.; Kemeny, L.; Kiss, E.; Kotyla, P.; Kovacs, L.; Kovalenko, V.; Kucharz, E.; Kwiatkowska, B.; Leszczynski, P.; Levchenko, E.; Lysenko, G.; Majdan, M.; Mihailov, C.; Nalotov, S.; Nedelciu, M.; Pavel, M.; Raskina, T.; Rebrov, B.; Rezus, E.; Semen, T.; Smakotina, S.; Stanislavchuk, M.; Stanislav, M.; Szombati, I.; Szucs, G.; Udrea, G.; Zajdel, J.; Zon‐Giebel, A.; Bonfiglioli, R.; Bustamante, R.; Klumb, E.; Ramirez, G. Medrano; Neiva, C.; Olguin, M.; Gonzaga, J.Reyes; Scotton, A.; Ayala, S. Sicsik; Ximenes, A.; Sharma, R.; Srikantiah, C.; Aelion, J.; Aranow, C.; Baker, M.; Chadha, A.; Chao, J.; Chatham, W.; Chow, A.; Clay, C.; Cohen‐Gadol, S.; Conaway, D.; Denburg, J.; Escalante, A.; Espinoza, L.; Fiechtner, J.; Fortin, I.; Fraser, A.; Furie, R.; Gladman, D.; Goddard, D.; Goldberg, M.; Gonzalez‐Rivera, R.; Gorman, J.; Griffin, R.; Haaland, D.; Halter, D.; Hemaiden, A.; Hobbs, K.; Joshi, V.; Lim, S.; Kalunian, K.; Karpouzas, G.; Khraishi, M.; Lafyatis, R.; Lee, S.; Lidman, R.; Lue, C.; Mohan, M.; Mease, P.; Mehta, C.; Mizutani, W.; Nami, A.; Nascimento, J.; Neuwelt, C.; Pappas, J.; Pope, J.; Porges, A.; Roane, G.; Rosenberg, D.; Ross, S.; Saadeh, C.; Scoville, C.; Sherrer, Y.; Solomon, M.; Surbeck, W.; Valenzuela, G.; Waller, P.; Alten, R.; Baerwald, C.; Bienvenu, B.; Bombardieri, S.; Braun, J.; Dival, L.; Espinosa, G.; Fernandez, I. Figueroa; Gomez‐Reino, J.; Gordon, C.; Hiepe, F.; Hopkinson, N.; Isenberg, D.; Jacobi, A.; Jorgensen, C.; Guern, V. Le; Paul, C.; Pego‐Reigosa, J. M.; Heredia, J. Rodriguez; Rubbert‐Roth, A.; Sabbadini, M.; Schroeder, J.; Schwarting, A.; Spieler, W.; Valesini, G.; Wollenhaupt, J.; Mendoza, A. Zea; Zouboulis, C.

    2017-01-01

    Objective Epratuzumab, a monoclonal antibody that targets CD22, modulates B cell signaling without substantial reductions in the number of B cells. The aim of this study was to report the results of 2 phase III multicenter randomized, double‐blind, placebo‐controlled trials, the EMBODY 1 and EMBODY 2 trials, assessing the efficacy and safety of epratuzumab in patients with moderately to severely active systemic lupus erythematosus (SLE). Methods Patients met ≥4 of the American College of Rheumatology revised classification criteria for SLE, were positive for antinuclear antibodies and/or anti–double‐stranded DNA antibodies, had an SLE Disease Activity Index 2000 (SLEDAI‐2K) score of ≥6 (increased disease activity), had British Isles Lupus Assessment Group 2004 index (BILAG‐2004) scores of grade A (severe disease activity) in ≥1 body system or grade B (moderate disease activity) in ≥2 body systems (in the mucocutaneous, musculoskeletal, or cardiorespiratory domains), and were receiving standard therapy, including mandatory treatment with corticosteroids (5–60 mg/day). BILAG‐2004 grade A scores in the renal and central nervous system domains were excluded. Patients were randomized 1:1:1 to receive either placebo, epratuzumab 600 mg every week, or epratuzumab 1,200 mg every other week, with infusions delivered for the first 4 weeks of each 12‐week dosing cycle, for 4 cycles. Patients across all 3 treatment groups also continued with their standard therapy. The primary end point was the response rate at week 48 according to the BILAG‐based Combined Lupus Assessment (BICLA) definition, requiring improvement in the BILAG‐2004 score, no worsening in the BILAG‐2004 score, SLEDAI‐2K score, or physician's global assessment of disease activity, and no disallowed changes in concomitant medications. Patients who discontinued the study medication were classified as nonresponders. Results In the EMBODY 1 and EMBODY 2 trials of epratuzumab, 793

  16. Biogasification of steam-exploded wheat straw by a two-phased digestion system%汽爆麦草的两相法生物气化

    Institute of Scientific and Technical Information of China (English)

    陈洪章; 王麾; 张爱军; 李佐虎

    2005-01-01

    蒸汽爆破作为一种新型的预处理方法被初次运用于麦草的两相气化系统,以碳酸氢铵作为补充氮源调节碳氮比,并在相同条件下以未汽爆麦草作对照试验.以汽爆麦草为原料时,水解反应器的pH值低于加入未汽爆麦草的pH值,并且在25 d后慢慢升至7;以两种麦草为原料时,甲烷反应器的pH都稳定在7左右,此时最适合甲烷生成.汽爆麦草的总固形物和挥发性固形物含量分别降低了57.5%和62.1%,下降率差不多是未汽爆麦草的2倍;纤维素和半纤维素的降解率也有了显著的提高,分别为63.0%和67.4%;木质素的降解率变化不大,都在5%左右.汽爆麦草的总产气量高于非汽爆麦草,并且一直保持较高的增长率,而非汽爆麦草的增长率一直较低;两种麦草产生的生物气中的甲烷含量大致相同,在水解反应器中后期为50%左右,在甲烷反应器中为70%左右.以上结果表明,汽爆可以促进麦草的降解,提高生物气的产量,是一种行之有效的预处理技术.%In the study, steam-explosion, a novel pretreatment method of substrate, was firstly introduced in the twophased digestion system. Ammonium bicarbonate was used as added nitrogen source to adjust C/N in the system. Under the same conditions, experiments using NPWS (non-pretreated wheat straw) were processed as the control. Results showed that pH value in the hydrolysis reactor using SEWS (Steam-exploded wheat straw) was lower and increased slowly to 7 in 25 days. In methanogenesis reactors using SEWS and NPWS, pH value was all around 7, which is most suitable to produce methane. In the digestion system using SEWS, the content of TS (Total solid) and VS (Volatile solid) decreased by 57.5% and by 62.1%, respectively, about twice as high as NWPS. The degradation rate of cellulose and semi-cellulose of SEWS were 63.0% and 67.4%, respectively, much higher than NPWS. The degradation rate of the lignin is similar and about 5 %. The total

  17. Mechanical behavior of shock-wave consolidated nano and micron-sized aluminum/silicon carbide and aluminum/aluminum oxide two-phase systems characterized by light and electron metallography

    Science.gov (United States)

    Alba-Baena, Noe Gaudencio

    This dissertation reports the results of the exploratory study of two-phase systems consisting of 150 microm diameter aluminum powder mechanically mixed with 30 nm and 30 microm diameter SiC and Al2O3 powders (in volume fractions of 2, 4, and 21 percent). Powders were mechanically mixed and green compacted to ˜80% theorical density in a series of cylindrical fixtures (steel tubes). The compacted arrangements were explosively consolidated using ammonium nitrate-fuel oil (ANFO) to form stacks of two-phase systems. As result, successfully consolidated cylindrical monoliths of 50 mm (height) x 32 mm (in diameter) were obtained. By taking advantage of the use of SWC (shock wave consolidation) and WEDM (wire-electric discharge machining), the heterogeneous systems were machined in a highly efficiency rate. The sample cuts used for characterization and mechanical properties testing, require the use of less that 10cc of each monolith, in consequence there was preserved an average of 60% of the obtained system monoliths. Consolidated test cylinders of the pure Al and two-phase composites were characterized by optical metallography and TEM. The light micrographs for the five explosively consolidated regimes: aluminum powder, nano and micron-sized Al/Al2O3 systems, and the nano and micron-sized Al/SiC systems exhibit similar ductility in the aluminum grains. Low volume fraction systems exhibit small agglomerations at the grain boundaries for the Al/Al2O3 system and the Al/SiC system reveal a well distributed phase at the grain boundaries. Large and partially bonded agglomerations were observable in the nano-sized high volume fraction (21%) systems, while the micron-sized Al/ceramic systems exhibit homogeneous distribution along the aluminum phase grains. TEM images showed the shock-induced dislocation cell structure, which has partially recrystallized to form a nano grain structure in the consolidated aluminum powder. Furthermore, the SiC nano-agglomerates appeared to have

  18. Crystalline fibrillar gel formation in aqueous surfactant-antioxidant system.

    Science.gov (United States)

    Joseph, Linet Rose; Tata, B V R; Sreejith, Lisa

    2015-08-01

    Cetyltrimethylammonium bromide (CTAB) is a well-known cationic surfactant capable to micellize into diverse morphologies in aqueous medium. We observed the formation of an opaque gel state from aqueous CTAB solution in the presence of the aromatic additive, para-coumaric acid (PCA). Optical microscopic images revealed the presence of large fibrils in the system at room temperature. Gel nature of the fibrils was confirmed by rheological measurements. Presence of interstitial water in the fibrils was recognized with Raman spectroscopy. On heating the sample above 30 (°) C, the fibrillar gel state changes to a transparent liquid state with Newtonian flow properties. Dynamic light scattering study hinted the presence of small micelles in the solution above 30 (°) C. Thus the system showed a temperature-dependent structural transition from opaque water-swollen gel to transparent micellar liquid. The formation of water-swollen fibrillar network is attributed to surfactant-additive intermolecular interactions in aqueous medium. Transition to micelle phase above 30 (°) C is related to Kraft transition which is observed at significantly lower temperature for CTAB in the absence of PCA. The structural features of PCA play a key role in promoting fibrillar network formation and elevating the Kraft transition in aqueous solution of CTAB.

  19. Advanced Aqueous Separation Systems for Actinide Partitioning

    Energy Technology Data Exchange (ETDEWEB)

    Nash, Kenneth L.; Clark, Sue; Meier, G Patrick; Alexandratos, Spiro; Paine, Robert; Hancock, Robert; Ensor, Dale

    2012-03-21

    One of the most challenging aspects of advanced processing of spent nuclear fuel is the need to isolate transuranium elements from fission product lanthanides. This project expanded the scope of earlier investigations of americium (Am) partitioning from the lanthanides with the synthesis of new separations materials and a centralized focus on radiochemical characterization of the separation systems that could be developed based on these new materials. The primary objective of this program was to explore alternative materials for actinide separations and to link the design of new reagents for actinide separations to characterizations based on actinide chemistry. In the predominant trivalent oxidation state, the chemistry of lanthanides overlaps substantially with that of the trivalent actinides and their mutual separation is quite challenging.

  20. P450cam biocatalysis in surfactant-stabilized two-phase emulsions.

    Science.gov (United States)

    Ryan, Jessica D; Clark, Douglas S

    2008-04-15

    Cytochrome P450 monooxygenases (P450s) are powerful biocatalysts that have the ability to oxidize a broad range of substrates, often at non-reactive carbon centers. However, incorporation of P450s into synthetic schemes has so far been limited to a few whole-cell transformations. P450 substrates are often hydrophobic and have low water solubility, limiting the amount of product that can be produced. To help overcome this limitation, we have examined P450cam activity in two-phase hexane/water emulsions with and without the anionic surfactant, bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT). Hydroxylation of camphor to hydroxycamphor by the three- component P450cam system was chosen as the model reaction, and regeneration of NADH was accomplished with yeast alcohol dehydrogenase (YADH). P450cam was activated in the surfactant-free emulsions, and addition of AOT improved the activity even further, at least over the range of camphor concentrations for which initial rates were readily measurable in all media. The largest observed rate enhancement was 4.5-fold. Nearly 50-times more product was formed in the surfactant-stabilized emulsions than was achieved in aqueous buffer, with total turnover numbers reaching 28,900 for P450cam and 11,800 for YADH. In the absence of surfactant, the two-phase reaction appeared to be mass-transfer limited, while inclusion of AOT alleviated transport limitations and/or afforded a larger interfacial area for P450 activation. The oxidation of hydroxycamphor to 2,5-diketocamphane was also observed, owing to the large concentration of hydroxycamphor relative to camphor in the aqueous phase of the two-phase emulsion. This competing reaction was accompanied by the uncoupled oxidation of NADH (i.e., NADH oxidation without formation of 2,5-diketocamphane), which reduced the availability of NADH for camphor oxidation and further limited the yield of hydroxycamphor in the two-phase emulsions. These results indicate that a surfactant

  1. Pumped, Two-Phase Heat-Transfer Loop

    Science.gov (United States)

    Edelstein, F.

    1986-01-01

    Two-phase heat-transfer system delivers coolant to equipment as liquid and removes it as vapor. Alternatively, system heats equipment by delivering vapor and removing condensed liquid. Two-phase scheme effective for heat transfer over long distances. Heat-transfer plates remove heat from or supply heat to equipment. If temperature of plate is high, valve opens liquid-supply line to plate, and cooling results. If plate temperature is low, valve opens liquid-suction line to plate, and heating ensues.

  2. Resolution of α-cyclohexyl-mandelic acid enantiomers by two-phase (O/W) recognition chiral extraction

    Institute of Scientific and Technical Information of China (English)

    TANG; KeWen; ZHANG; GuoLi; HUANG; KeLong; LI; Yuanjian; YI; JianMin

    2007-01-01

    This paper presents a new chiral separation technology: two-phase (O/W) recognition chiral extraction. Distribution behavior of α-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-isobutyl tartrate in 1,2-dichloroethane organic phase and β-CD derivatives in aqueous phase, and the influence of the kind and concentration of extractant and pH on extraction performance was investigated. The experimental results indicate that two-phase (O/W) recognition chiral extraction is of strong chiral separation ability. HP-β-CD, HE-β-CD and Me-β-CD have higher recognition ability for S-CHMA than that for R-CHMA, among which HP-β-CD has the strongest ability; whereas, D-isobutyl tartrate has reversed recognition ability for them. In the extraction system containing HP-β-CD and D-isobutyl tartrate, e.e.% of S-CHMA in aqueous phase reached 27.6% by one stage extraction, and the distribution ratio for R-CHMA(kR) and for S-CHMA(kS) and separation factor (α) are 2.44, 0.89 and 2.49, respectively. Meanwhile, pH and concentration of extractant have great effects on chiral separation ability. Two-phase (O/W) recognition chiral extraction has great significance for preparative separation of racemic compounds.

  3. Effects of Geometric Parameter on Performance of Ejector and Two-phase Ejector Refrigeration System%几何参数对引射器及两相流引射制冷系统性能的影响

    Institute of Scientific and Technical Information of China (English)

    裴文伟; 郭宪民; 王善云; 郭欣炜

    2013-01-01

    In order to increase the coefficient of performance (COP) of the refrigeration system , the expansion valve is replaced by a two-phase ejector in the two-phase ejector refrigeration cycle to recover the potential energy of the high pressure refrigerant. The performance of two-phase ejector refrigeration system with refrigerant R134a as working fluid is experimentally investigated. The effects of the throat diameter of the nozzle and the diameter of the mixing chamber on the performance of the ejector and the two-phase ejector refrigeration cycle system were analyzed. The experimental results indicate that there is an optimal combination of the nozzle throat diameter and the mixing chamber diameter to maximize the entrainment ratio under a fixed working condition. The entrainment ratio of the ejector reaches to the maximum value with the throat diameter of 2.0mm and the mixing chamber diameter of 16mm under the conditions of 3℃ evaporating temperature and 55℃ condensing temperature. Under a fixed working condition, the optimal combination of the nozzle throat diameter and the mixing chamber diameter for maximum of the entrainment ratio is not consistent with the one for maximum of the COP of the refrigeration system. This may be caused by the shocks in the ejector, which should be investigated deeply.%  用两相流引射器代替膨胀阀,可回收两相流引射制冷循环中高压工质的压力能,提高制冷系统效率.对以R134a为工质的两相流引射制冷系统性能进行了实验研究,分析了喷嘴喉部直径和混合室直径对R134a两相流引射器及引射制冷系统性能的影响.实验结果表明,在固定工况条件下,存在使引射比达到最大的最佳喷嘴喉部直径和混合室直径组合.在蒸发温度为3℃、冷凝温度为55℃的工况下,当喷嘴喉部直径为2.0mm、混合室直径为16mm时引射器的引射比最大.在固定工况条件下,使引射比达到最大值的喷嘴喉部直

  4. Study of two-phase flows in reduced gravity

    Science.gov (United States)

    Roy, Tirthankar

    Study of gas-liquid two-phase flows under reduced gravity conditions is extremely important. One of the major applications of gas-liquid two-phase flows under reduced gravity conditions is in the design of active thermal control systems for future space applications. Previous space crafts were characterized by low heat generation within the spacecraft which needed to be redistributed within the craft or rejected to space. This task could easily have been accomplished by pumped single-phase loops or passive systems such as heat pipes and so on. However with increase in heat generation within the space craft as predicted for future missions, pumped boiling two-phase flows are being considered. This is because of higher heat transfer co-efficients associated with boiling heat transfer among other advantages. Two-phase flows under reduced gravity conditions also find important applications in space propulsion as in space nuclear power reactors as well as in many other life support systems of space crafts. Two-fluid model along with Interfacial Area Transport Equation (IATE) is a useful tool available to predict the behavior of gas-liquid two-phase flows under reduced gravity conditions. It should be noted that considerable differences exist between two-phase flows under reduced and normal gravity conditions especially for low inertia flows. This is because due to suppression of the gravity field the gas-liquid two-phase flows take a considerable time to develop under reduced gravity conditions as compared to normal gravity conditions. Hence other common methods of analysis applicable for fully developed gas-liquid two-phase flows under normal gravity conditions, like flow regimes and flow regime transition criteria, will not be applicable to gas-liquid two-phase flows under reduced gravity conditions. However the two-fluid model and the IATE need to be evaluated first against detailed experimental data obtained under reduced gravity conditions. Although lot of studies

  5. INFLUENCE OF SURFACTANT ON TWO-PHASE FLOW REGIME AND PRESSURE DROP IN UPWARD INCLINED PIPES

    Institute of Scientific and Technical Information of China (English)

    XIA Guo-dong; CHAI Lei

    2012-01-01

    The influence of a surfactant on the two-phase flow regime and the pressure drop in upward inclined pipes is investigated for various gas/liquid flow rates.The air/water and air/100 ppm sodium dodecyl sulphate aqueous solution are used as the working fluids.The influence of the surfactant on the two-phase flow regime in upward inclined pipes is investigated using the electrical tomographic technique.For 0°,2.5° and 5° pipe inclinations,the surfactant has obvious effect on the transition from the stratified wavy flow to the annular flow,and the range of the stratified smooth flow regime is also extended to higher gas velocities.For 10°pipe inclination,no stratified flow regime is observed in the air/water flow.In the air/surfactant solution system,however,the stratified flow regime can be found in the range of USG =10m/s-28m/s and USL =0.07 m/s-0.2 m/s.For all inclination angles,the changes of the pressure gradient characteristics are accompanied with the flow pattern transitions.Adding surfactant in a two-phase flow would reduce the pressure gradient significantly in the slug flow and annular flow regimes.In the annular flow regime,the pressure gradient gradually becomes free of the influence of the upward inclined angle,and is only dependent on the property of the two-phase flow.

  6. 48 CFR 36.301 - Use of two-phase design-build selection procedures.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Use of two-phase design... ACQUISITION REGULATION SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACTS Two-Phase Design-Build Selection Procedures 36.301 Use of two-phase design-build selection procedures....

  7. 48 CFR 570.105-2 - Two-phase design-build selection procedures.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Two-phase design-build..., you must use the two-phase design-build selection procedures in section 303M of the Federal Property... use of the two-phase selection procedures. (v) The capability of the agency to manage the...

  8. Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli.

    Science.gov (United States)

    Gallego-Jara, Julia; de Diego, Teresa; Del Real, Álvaro; Écija-Conesa, Ana; Manjón, Arturo; Cánovas, Manuel

    2015-12-01

    Lycopene is an import ant compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic solvents was found to maximize lycopene production through in situ extraction from cells. Within the reactor, three phases were formed during the process: an aqueous phase containing the recombinant E. coli, an interphase, and an organic phase. Lycopene was extracted from the cells to both the interphase and the organic phase and, consequently, thus enhancing its production. Maximum lycopene production (74.71 ± 3.74 mg L(-1)) was obtained for an octane-aqueous culture system using the E. coli BL21LF strain, a process that doubled the level obtained in the control aqueous culture. Study of the interphase by transmission electron microscopy (TEM) showed the proteo-lipidic nature and the high storage capacity of lycopene. Moreover, a cell viability test by flow cytometry (CF) after 24 h of culture indicated that 24 % of the population could be re-used. Therefore, a batch series reactor was designed for semi-continuous lycopene extraction. After five cycles of operation (120 h), lycopene production was similar to that obtained in the control aqueous medium. A final specific lycopene yield of up to 49.70 ± 2.48 mg g(-1) was reached at 24 h, which represents to the highest titer to date. In conclusion, the aqueous-organic semi-continuous culture system proposed is the first designed for lycopene extraction, representing an important breakthrough in the development of a competitive biotechnological process for lycopene production and extraction.

  9. Velocity and energy relaxation in two-phase flows

    CERN Document Server

    Meyapin, Yannick; Gisclon, Marguerite

    2009-01-01

    In the present study we investigate analytically the process of velocity and energy relaxation in two-phase flows. We begin our exposition by considering the so-called six equations two-phase model [Ishii1975, Rovarch2006]. This model assumes each phase to possess its own velocity and energy variables. Despite recent advances, the six equations model remains computationally expensive for many practical applications. Moreover, its advection operator may be non-hyperbolic which poses additional theoretical difficulties to construct robust numerical schemes |Ghidaglia et al, 2001]. In order to simplify this system, we complete momentum and energy conservation equations by relaxation terms. When relaxation characteristic time tends to zero, velocities and energies are constrained to tend to common values for both phases. As a result, we obtain a simple two-phase model which was recently proposed for simulation of violent aerated flows [Dias et al, 2010]. The preservation of invariant regions and incompressible li...

  10. Stabilized aqueous foam systems, concentrate for producing a stabilized aqueous foam and method of producing said foam

    Energy Technology Data Exchange (ETDEWEB)

    Rand, P.B.

    This invention comprises a combination of a water soluble polymer of the polyacrylic acid type, a foam stabilizer of dodecyl alcohol, a surfactant, a solvent and water as a concentrate for use in producing stabilized aqueous foams. In another aspect, the invention comprises a solution of the concentrate with water. In still another aspect the invention includes a method of generating stabilized aqueous foams. The stable foams have utility in security systems.

  11. Separation of Nanoparticles in Aqueous Multiphase Systems through Centrifugation

    KAUST Repository

    Akbulut, Ozge

    2012-08-08

    This paper demonstrates the use of aqueous multiphase systems (MuPSs) as media for rate-zonal centrifugation to separate nanoparticles of different shapes and sizes. The properties of MuPSs do not change with time or during centrifugation; this stability facilitates sample collection after separation. A three-phase system demonstrates the separation of the reaction products (nanorods, nanospheres, and large particles) of a synthesis of gold nanorods, and enriches the nanorods from 48 to 99% in less than ten minutes using a benchtop centrifuge. © 2012 American Chemical Society.

  12. Two-Phase Cavitating Flow in Turbomachines

    Directory of Open Access Journals (Sweden)

    Sandor I. Bernad

    2012-11-01

    Full Text Available Cavitating flows are notoriously complex because they are highly turbulent and unsteady flows involving two species (liquid/vapor with a large density difference. These features pose a unique challenge to numerical modeling works. The study briefly reviews the methodology curently employed for industrial cavitating flow simulations using the two-phase mixture model. The two-phase mixture model is evaluated and validated using benchmark problem where experimental data are available. A 3D cavitating flow computation is performed for the GAMM Francis runner. The model is able to qualitatively predict the location and extent of the 3D cavity on the blade, but further investigation are needed to quatitatively assess the accuracy for real turbomachinery cavitating flows.

  13. 超声波辅助双水相提取条斑紫菜黄酮类物质及其抗氧化活性研究%Ultrasonic-Assisted Aqueous Two-Phase Extraction and Antioxidant Activity of Total Flavonoids from

    Institute of Scientific and Technical Information of China (English)

    陈利梅

    2012-01-01

    An ultrasonic-assisted aqueous two phase extraction method was presented for extracting total flavonoids from Porphyra yezoensisand the extraction conditions were optimized by an orthogonal array experimental design. Meanwhile, the hydroxyl free radical scavenging activity of total flavonoids from Porphyra yezoensis was determined. The results indicated that the method allowed effective extraction of total flavonoids. The extraction yield was 0.148%, which was significantly higher than that obtained by ethanol-water reflux extraction. The optimal extraction conditions were obtained as follows: n-propanol-to- water ratio 0.6, ammonium sulphate concentration 0.30 g/mL, ultrasonic treatment time 20 rain and material-to-liquid ratio 0.04. P. yezoensis abtmdanfly contained flavonoids and the total flavonoid extract from it had excellent hydroxyl free radical scavenging activity thus providing a good natural free radical scavenger.%利用超声波辅助丙醇-硫酸铵双水相体系提取紫菜中总黄酮,并优化提取条件,测定总黄酮提取物对羟自由基(·OH)的抑制活性。结果表明:本方法可有效地从紫菜中提取总黄酮,紫菜总黄酮提取率为0.148%,明显高于乙醇-水回流提取法。最优提取条件为醇水比为0.6、硫酸铵质量浓度0.30g/mL、超声时间20min、料液比0.04。紫菜中含有较丰富的黄酮类物质,提取物对·OH具有良好的抑制作用,是一种有效的天然自由基清除剂,具有很大的开发利用前景。

  14. Critical thinking: a two-phase framework.

    Science.gov (United States)

    Edwards, Sharon L

    2007-09-01

    This article provides a comprehensive review of how a two-phase framework can promote and engage nurses in the concepts of critical thinking. Nurse education is required to integrate critical thinking in their teaching strategies, as it is widely recognised as an important part of student nurses becoming analytical qualified practitioners. The two-phase framework can be incorporated in the classroom using enquiry-based scenarios or used to investigate situations that arise from practice, for reflection, analysis, theorising or to explore issues. This paper proposes a two-phase framework for incorporation in the classroom and practice to promote critical thinking. Phase 1 attempts to make it easier for nurses to organise and expound often complex and abstract ideas that arise when using critical thinking, identify more than one solution to the problem by using a variety of cues to facilitate action. Phase 2 encourages nurses to be accountable and responsible, to justify a decision, be creative and innovative in implementing change.

  15. On the modelling and the analysis of two-phase flow instabilities in pipeline-riser systems; Modelisation et analyse des instabilites d'ecoulements diphasiques dans les conduites petrolieres du type pipeline-riser

    Energy Technology Data Exchange (ETDEWEB)

    Zakarian, E.

    2000-03-10

    Recent estimations predict that over half the remaining offshore oil and gas reserves are located in deep water and marginal fields. For such reserves, economic recovery methods are required. Then, multiphase flows are transported within pipelines and separated on treatment platforms built in shallow water or processed in onshore facilities. Unfortunately, hydrodynamic instabilities may occur whenever gas and liquid flow in a pipeline, generating serious operating problems. This dissertation presents a new way to model two-phase flows in pipelines such as pipeline-riser systems. Equations are algebraic and differential. Their smoothness depends on the closure laws of the problem such as slip or friction laws. Smooth forms of these closure laws are presented for the first time in this dissertation. Therefore, a mathematical analysis of our model fits into a classical frame: a linear analysis leads to an analytical expression of the boundary between stable and unstable flows. A nonlinear analysis provides for the first time, the bifurcation curves of gas-liquid flows in pipe-riser systems, locally round their stability boundary. (author)

  16. Thermoreversible (Ionic-Liquid-Based) Aqueous Biphasic Systems.

    Science.gov (United States)

    Passos, Helena; Luís, Andreia; Coutinho, João A P; Freire, Mara G

    2016-02-04

    The ability to induce reversible phase transitions between homogeneous solutions and biphasic liquid-liquid systems, at pre-defined and suitable operating temperatures, is of crucial relevance in the design of separation processes. Ionic-liquid-based aqueous biphasic systems (IL-based ABS) have demonstrated superior performance as alternative extraction platforms, and their thermoreversible behaviour is here disclosed by the use of protic ILs. The applicability of the temperature-induced phase switching is further demonstrated with the complete extraction of two value-added proteins, achieved in a single-step. It is shown that these temperature-induced mono(bi)phasic systems are significantly more versatile than classical liquid-liquid systems which are constrained by their critical temperatures. IL-based ABS allow to work in a wide range of temperatures and compositions which can be tailored to fit the requirements of a given separation process.

  17. Two-phase Flow Patterns in High Temperature Generator of Absorption Chiller / Heater

    Science.gov (United States)

    Furukawa, Masahiro; Kanuma, Hitoshi; Sekoguchi, Kotohiko; Takeishi, Masayuki

    There is a lack of information about vapor-liquid two-phase flow patterns determined using void signals in high temperature generator of absorption chiller/heater. Sensing void fraction has been hampered because lithium bromide aqueous solution of strong alkalinity is employed as working fluid at high temperature and high level of vacuum. New void sensor applicable to such difficult conditions was developed. The void Fractions at 48 locations in a high temperature generator were measured simultaneously in both cooling and heating operations. Analysis of void signals detected reveals that the most violent boiling occurs at the upper part of rear plate of combustion chamber and the first line of vertical tubes located in the flue. The flow patterns are strongly affected by the system pressure difference between the cooling and heating operations: there appear bubbly, slug and froth flows in the cooling operation, but only bubbly flow in the heating operation.

  18. Analysis of Direct Samples of Early Solar System Aqueous Fluids

    Science.gov (United States)

    Zolensky, Michael E.; Bodnar, R J.; Fedele, L.; Yurimoto,H.; Itoh, S.; Fries, M.; Steele, A.

    2012-01-01

    Over the past three decades we have become increasingly aware of the fundamental importance of water, and aqueous alteration, on primitive solar-system bodies. Some carbonaceous and ordinary chondrites have been altered by interactions with liquid water within the first 10 million years after formation of their parent asteroids. Millimeter to centimeter-sized aggregates of purple halite containing aqueous fluid inclusions were found in the matrix of two freshly-fallen brecciated H chondrite falls, Monahans (1998, hereafter simply "Monahans") (H5) and Zag (H3-6) (Zolensky et al., 1999; Whitby et al., 2000; Bogard et al., 2001) In order to understand origin and evolution of the aqueous fluids inside these inclusions we much measure the actual fluid composition, and also learn the O and H isotopic composition of the water. It has taken a decade for laboratory analytical techniques to catch up to these particular nanomole-sized aqueous samples. We have recently been successful in (1) measuring the isotopic composition of H and O in the water in a few fluid inclusions from the Zag and Monahans halite, (2) mineralogical characterization of the solid mineral phases associated with the aqueous fluids within the halite, and (3) the first minor element analyses of the fluid itself. A Cameca ims-1270 equipped with a cryo-sample-stage of Hokkaido University was specially prepared for the O and H isotopic measurements. The cryo-sample-stage (Techno. I. S. Corp.) was cooled down to c.a. -190 C using liquid nitrogen at which the aqueous fluid in inclusions was frozen. We excavated the salt crystal surfaces to expose the frozen fluids using a 15 keV Cs+ beam and measured negative secondary ions. The secondary ions from deep craters of approximately 10 m in depth emitted stably but the intensities changed gradually during measurement cycles because of shifting states of charge compensation, resulting in rather poor reproducibility of multiple measurements of standard fluid

  19. Aqueous biphasic systems involving alkylsulfate-based ionic liquids

    Energy Technology Data Exchange (ETDEWEB)

    Deive, Francisco J. [Instituto de Tecnologia Quimica e Biologica, UNL, Av. Republica, Apartado 127, 2780-901 Oeiras (Portugal); Department of Chemical Engineering, University of Vigo, P.O. Box 36310, Vigo (Spain); Rodriguez, Ana [Department of Chemical Engineering, University of Vigo, P.O. Box 36310, Vigo (Spain); Marrucho, Isabel M., E-mail: imarrucho@itqb.unl.pt [Instituto de Tecnologia Quimica e Biologica, UNL, Av. Republica, Apartado 127, 2780-901 Oeiras (Portugal); Rebelo, Luis P.N. [Instituto de Tecnologia Quimica e Biologica, UNL, Av. Republica, Apartado 127, 2780-901 Oeiras (Portugal)

    2011-11-15

    Highlights: > K{sub 3}PO{sub 4}, K{sub 2}CO{sub 3}, Na{sub 2}CO{sub 3}, and (NH{sub 4}){sub 2}SO{sub 4} act as phase promoter in aqueous solutions of ILs. > Remarkable influence of alkyl-chain length on solubility curves of alkylsulfate-based ILs. > Merchuck correlation was used for describing these systems. > {Delta}S{sub hyd} and Hofmeister series were used to discuss the different salting out effects. - Abstract: The specific effects of K{sub 3}PO{sub 4}, K{sub 2}CO{sub 3}, Na{sub 2}CO{sub 3}, and (NH{sub 4}){sub 2}SO{sub 4}, as high charge-density inorganic salts and thus inducers of the formation of aqueous biphasic systems (ABS) containing several ethyl-methylimidazolium alkylsulfate ionic liquids, C{sub 2}MIM C{sub n}SO{sub 4} (n = 2, 4, 6, or 8), have been assessed at T = 298.15 K. The results are analyzed in the light of the Hofmeister series. The influence of different alkyl chain lengths in the anion, together with the ability of the selected inorganic salts to induce the formation of ABS, is discussed. Phase diagrams have been determined through turbidimetry, including tie lines assignments from mass phase ratios according to the lever - arm rule. The Merchuck equation was satisfactorily used to correlate the solubility curve.

  20. DOE workshop: Sedimentary systems, aqueous and organic geochemistry

    Energy Technology Data Exchange (ETDEWEB)

    1993-07-01

    A DOE workshop on sedimentary systems, aqueous and organic geochemistry was held July 15-16, 1993 at Lawrence Berkeley Laboratory. Papers were organized into several sections: Fundamental Properties, containing papers on the thermodynamics of brines, minerals and aqueous electrolyte solutions; Geochemical Transport, covering 3-D imaging of drill core samples, hydrothermal geochemistry, chemical interactions in hydrocarbon reservoirs, fluid flow model application, among others; Rock-Water Interactions, with presentations on stable isotope systematics of fluid/rock interaction, fluid flow and petotectonic evolution, grain boundary transport, sulfur incorporation, tracers in geologic reservoirs, geothermal controls on oil-reservoir evolution, and mineral hydrolysis kinetics; Organic Geochemistry covered new methods for constraining time of hydrocarbon migration, kinetic models of petroleum formation, mudstones in burial diagenesis, compound-specific carbon isotope analysis of petroleums, stability of natural gas, sulfur in sedimentary organic matter, organic geochemistry of deep ocean sediments, direct speciation of metal by optical spectroscopies; and lastly, Sedimentary Systems, covering sequence stratigraphy, seismic reflectors and diagenetic changes in carbonates, geochemistry and origin of regional dolomites, and evidence of large comet or asteroid impacts at extinction boundaries.

  1. Enhancement of a novel extracellular uricase production by media optimization and partial purification by aqueous three-phase system.

    Science.gov (United States)

    Ram, Senthoor K; Raval, Keyur; JagadeeshBabu, P E

    2015-01-01

    Uricase (urate: oxygen oxidoreductase, EC 1.7.3.3), an enzyme belonging to the class of oxidoreductases, catalyzes the enzymatic oxidation of uric acid to allantoin and finds a wide variety of application as therapeutic and clinical reagent. In this study, uricase production ability of the bacterial strains isolated from deep litter poultry soil is investigated. The strain with maximum extracellular uricase production capability was identified as Xanthomonas fuscans subsp. aurantifolii based on 16S rRNA sequencing. Effect of various carbon and nitrogen sources on uricase productivity was investigated. The uricase production for this strain was optimized using statistically based experimental designs and resulted in uricase activity of 306 U/L, which is 2 times higher than initial uricase activity. Two-step purification, such as ammonium sulfate precipitation and aqueous two-phase system, was carried out and a twofold increase in yield and specific activity was observed.

  2. Milking microalga Dunaliella salina for Beta-carotene production in two-phase bioreactors

    NARCIS (Netherlands)

    Hejazi, M.; Holwerda, E.; Wijffels, R.H.

    2004-01-01

    A new method was developed for production of beta-carotene from Dunaliella salina. Cells were grown in low light intensity and then transferred to a production bioreactor illuminated at a higher light intensity. It was a two-phase bioreactor consisting of an aqueous and a biocompatible organic phase

  3. pH-distribution of cerium species in aqueous systems

    Institute of Scientific and Technical Information of China (English)

    B.Bouchaud; J.Balmain; G.Bonnet; F.Pedraza

    2012-01-01

    Cerium-based oxide coalings can be obtained through either chemical or electrochemical processes on various conductor and semiconductor substrates.In both cases the films develop through a precipitation mechanism,which strongly depends on the solution chemistry.In the particular case of the electrolytic approach,the elaboration parameters play a key role on the interfacial pH modification thereby leading to an indirect precipitation mechanism.Indeed,the nucleation and growth mechanisms of crystallites and the composition of the resulting layers have been shown to be also strongly affected by the deposition conditions as well as by the substrate composition,which could in turn modify the protectiveness provided by such coatings.Therefore a better fundamental understanding of the system is required,in particular of the distribution of cerium-containing species in aqueous solution.To this end,the present work intended to develop a diagram showing the distribution as well as the relative amount of Ce(Ⅲ)/Ce(Ⅳ) species in aqueous media as a function of the pH range.The resulting pH-distribution diagram turned out to be a useful tool to predict the relevant precipitation mechanisms and species involved during the growth of cerium-containing films and to draw correlations with the characteristics of the as-deposited films.

  4. Photodegradation of chlorbromuron, atrazine, and alachlor in aqueous systems under solar irradiation

    Directory of Open Access Journals (Sweden)

    Cristina Lizama Bahena

    2006-01-01

    Full Text Available Homogeneous and heterogeneous aqueous systems of the herbicides of chlorbromuron, atrazine, and alachlor were irradiated with a nonexpensive solar irradiation using a photoreactor with recirculation. Photodegradation of these herbicides occurred in both aqueous systems; however the presence of TiO2 clearly accelerated the degradation of the three herbicides in comparison with direct photolysis. Degradation was followed by measuring the chemical oxygen demand (COD as a function of reaction time for each aqueous system. Over 90% of COD abatement in the heterogeneous aqueous system was obtained in a short time period showing that mineralization of chlorbromuron, atrazine, and alachlor was achieved.

  5. Rheological properties of aqueous Pluronic-alginate systems containing liposomes.

    Science.gov (United States)

    Grassi, G; Crevatin, A; Farra, R; Guarnieri, G; Pascotto, A; Rehimers, B; Lapasin, R; Grassi, M

    2006-09-01

    Rheological and erosion studies regarding a liposome-containing polymeric blend that is propaedeutic to its use in paving techniques in tubular organs, such as blood vessels, are reported. Attention is focused on an aqueous polymeric blend composed of Pluronic (PF127) and alginate (Protanal LF 10/60) because both polymers, when dissolved in water at a sufficiently high concentration, are subjected to different structural mechanisms, which are driven by temperature increase and addition of bivalent cations, respectively, and both result in marked viscoelastic and plastic properties. After proving the compatibility between PF127 and alginate, we show that the structural transition temperature of the blend, T(ST), can be properly modulated. In particular, we found that T(ST) for an aqueous solution of pure Pluronic 20% w/w is about 21 degrees C and that even slight reductions in polymer concentration result in considerable T(ST) decrease. The addition of salts or alginate (provided as Na-alginate) provokes a substantial decrease of T(ST) and thus the alginate concentration in the blend should not exceed 1% w/w. In addition, liposomes slow down the structural transition but do not substantially affect the rheological properties of the system in the final state at higher temperatures, thus showing that they can be added to the polymeric blend without significant effects. Finally, erosion tests show that after contact with a source of bivalent cations, the polymeric blend containing PF127 and alginate shows an erosion resistance neatly improved with respect to the simple structured Pluronic system having the same polymer concentration. As a whole, all these results constitute the basis for future potential applications of the considered polymeric blend in tubular organs such as blood vessels.

  6. Radiation-Engineered Functional Nanoparticles in Aqueous Systems.

    Science.gov (United States)

    Dispenza, Clelia; Grimaldi, Natascia; Sabatino, Maria Antonietta; Soroka, Inna L; Jonsson, Mats

    2015-05-01

    Controlled synthesis of nanoscalar and nanostructured materials enables the development of novel functional materials with fine-tuned optical, mechanical, electronic, magnetic, conductive and catalytic properties that are of use in numerous applications. These materials have also found their potential use in medicine as vehicles for drug delivery, in diagnostics or in combinations thereof. In principle, nanoparticles can be divided into two broad categories, organic and inorganic nanoparticles. For both types of nanoparticles there are numerous possible synthetic routes. Considering the large difference in nature of these materials and the elementary reactions involved in the synthetic routes, most manufacturing techniques are complex and only suitable for one type of particle. Interestingly, radiation chemistry, i.e., the use of ionizing radiation from radioisotopes and accelerators to induce nanomaterials or chemical changes in materials, has proven to be a versatile tool for controlled manufacturing of both organic and inorganic nanoparticles. The advantages of using radiation chemistry for this purpose are many, such as low energy consumption, minimal use of potentially harmful chemicals and simple production schemes. For medical applications one more advantage is that the material can be sterile as manufactured. Radiation-induced synthesis can be carried out in aqueous systems, which minimizes the use of organic solvents and the need for separation and purification of the final product. The radiation chemistry of water is well known, as are the various ways of fine-tuning the reactivity of the system towards a desired target by adding different solutes. This, in combination with the controllable and adjustable irradiation process parameters, makes the technique superior to most other chemical methods. In this review, we discuss the fundamentals of radiation chemistry and radiation-induced synthesis of nanoparticles in aqueous solutions. The impact of dose and

  7. Droplet Manipulations in Two Phase Flow Microfluidics

    Directory of Open Access Journals (Sweden)

    Arjen M. Pit

    2015-11-01

    Full Text Available Even though droplet microfluidics has been developed since the early 1980s, the number of applications that have resulted in commercial products is still relatively small. This is partly due to an ongoing maturation and integration of existing methods, but possibly also because of the emergence of new techniques, whose potential has not been fully realized. This review summarizes the currently existing techniques for manipulating droplets in two-phase flow microfluidics. Specifically, very recent developments like the use of acoustic waves, magnetic fields, surface energy wells, and electrostatic traps and rails are discussed. The physical principles are explained, and (potential advantages and drawbacks of different methods in the sense of versatility, flexibility, tunability and durability are discussed, where possible, per technique and per droplet operation: generation, transport, sorting, coalescence and splitting.

  8. DESIGN OF ROBOTIC COLONIZER CONTROL SYSTEM FOR AQUEOUS ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    C.VENKATESH

    2013-05-01

    Full Text Available Now a days there is a huge interest on underwater communication systems for various applications in order to explore aqueous environments. Intelligent robots and cooperative multi- agent robotic systems can be very efficient tools to speed up search and research operations in remote areas. Robots are also useful to do jobs inareas and in situations that are hazardous for human, they can go anywhere that is not reachable my humans and can go into gaps and move trough small holes that are impossible for humans and even trained dogs. in this paper, a wireless underwater mobile robot system is designed in order to study the behavior of artemia group. anew idea has been presented for underwater mobile robot system which is consists of two parts, first is the underwater mechanical robot and the second is ZigBee wireless based mobile robot which controls and moves the first part. by this system different patterns motion control (linear, circular, zigzag, etc. has been performed and proved the ability to control group of robot by controlling the group of artemia and monitoring the underwater mobile robot control with the help of water proof RF wireless camera and also explore the details present around the mobile robot

  9. 一种新型两相励磁开关磁阻电机驱动系统的静态特性%STATIC PERFORMANCE OF A NEW SRD SYSTEM WITH TWO-PHASE EXCITATION

    Institute of Scientific and Technical Information of China (English)

    郭伟; 詹琼华; 丘亦慧

    2002-01-01

    该文主要分析了一种两相同时励磁的开关磁阻电机(SRM)的静态特性.文中首先简要介绍了这种两相励磁系统的结构,然后提出了两相励磁下SRM的磁链模型.基于详细的非线性有限元分析(FEA)的结果,建立了简化的线性模型且利用此线性模型导出了SRM的转矩--转角特性,并和非线性FEA模型下的结果进行了比较.最后,根据FEA的结果,提出了一种优化的控制策略来提高这种SRM的性能.%This paper provides an analysis of a switched reluctance motor (SRM) under two-phase excitation. After a brief introduction of the two-phase excitation scheme, the flux linkage model of a SRM under two-phase excitation is developed using FEA. A linearized model is then established based on the detailed flux linkage analysis. The torque-angle characteristics of such a linear model are compared with that derived from the detailed and nonlinear FEA model. Based on theFEA model, an optimized control strategy is proposed to improve the performanceof the SRM under two-phase excitation.

  10. For solid-liquid two phase softness abrasive flow embedded real-time monitoring and control system%面向固-液两相软性磨粒流嵌入式实时测控系统

    Institute of Scientific and Technical Information of China (English)

    计时鸣; 兰信鸿

    2012-01-01

    In order to solve the problems of flow pipe is to small to the conventional sensor for solid-liquid two phase softness abrasive flow,a monitoring method based on the vibration signal was proposed. Through the wavelet packet analysis of vibration signal, extraction signal characteristics were gotten. Using object-oriented technology, a kind of measurement and control software framework was developed, combined with finite state machine theory and embedded real-time operating system and programming methods of multitasking. Using Linux and DSP/BIOS RTOS, the double operating system signal processing technology was implemented,a multi-channel high speed data acquisition function was implemented on the intelligence embedded real-time measurement and control platform. The results indicate that it is easy to expand and interactive.%针对固-液两相软性磨粒流精密抛光方法中流道微小,无法采用常规直接信号传感器的问题,提出了一种基于二次信号-加速度振动信号的软性磨粒流监测方法.通过小波包分析提取了振动信号的特征.结合基于有限状态机理论与基于嵌入式实时操作系统的并发多任务的程序设计方法,采用面向对象技术开发了一种测控软件框架.采用Linux和DSP/BIOS RTOS实施了双操作系统信号处理技术,开发实现了一个面向软性磨粒流的,具有通用多通道高速数据采集功能的智能化嵌入式实时测控平台.研究结果表明,该系统具有易扩展与易交互的优点.

  11. ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT; ULTRASONIC AQUEOUS CLEANING SYSTEMS, SMART SONIC CORPORATION, SMART SONIC

    Science.gov (United States)

    This report is a product of the U.S. EPA's Environmental Technoloy Verification (ETV) Program and is focused on the Smart Sonics Ultrasonic Aqueous Cleaning Systems. The verification is based on three main objectives. (1) The Smart Sonic Aqueous Cleaning Systems, Model 2000 and...

  12. Two-Phase Quality/Flow Meter

    Science.gov (United States)

    Moerk, J. Steven (Inventor); Youngquist, Robert C. (Inventor); Werlink, Rudy J. (Inventor)

    1999-01-01

    A quality and/or flow meter employs a capacitance probe assembly for measuring the dielectric constant of flow stream, particularly a two-phase flow stream including liquid and gas components.ne dielectric constant of the flow stream varies depending upon the volume ratios of its liquid and gas components, and capacitance measurements can therefore be employed to calculate the quality of the flow, which is defined as the volume ratio of liquid in the flow to the total volume ratio of gas and liquid in the flow. By using two spaced capacitance sensors, and cross-correlating the time varying capacitance values of each, the velocity of the flow stream can also be determined. A microcontroller-based processing circuit is employed to measure the capacitance of the probe sensors.The circuit employs high speed timer and counter circuits to provide a high resolution measurement of the time interval required to charge each capacitor in the probe assembly. In this manner, a high resolution, noise resistant, digital representation of each of capacitance value is obtained without the need for a high resolution A/D converter, or a high frequency oscillator circuit. One embodiment of the probe assembly employs a capacitor with two ground plates which provide symmetry to insure that accurate measurements are made thereby.

  13. Reticulation of Aqueous Polyurethane Systems Controlled by DSC Method

    Directory of Open Access Journals (Sweden)

    Jakov Stamenkovic

    2006-06-01

    Full Text Available The DSC method has been employed to monitor the kinetics of reticulation ofaqueous polyurethane systems without catalysts, and with the commercial catalyst of zirconium(CAT®XC-6212 and the highly selective manganese catalyst, the complex Mn(III-diacetylacetonemaleinate (MAM. Among the polyol components, the acrylic emulsions wereused for reticulation in this research, and as suitable reticulation agents the water emulsiblealiphatic polyisocyanates based on hexamethylendoisocyanate with the different contents ofNCO-groups were employed. On the basis of DSC analysis, applying the methods of Kissinger,Freeman-Carroll and Crane-Ellerstein the pseudo kinetic parameters of the reticulation reactionof aqueous systems were determined. The temperature of the examination ranged from 50oC to450oC with the heat rate of 0.5oC/min. The reduction of the activation energy and the increaseof the standard deviation indicate the catalytic action of the selective catalysts of zirconium andmanganese. The impact of the catalysts on the reduction of the activation energy is thestrongest when using the catalysts of manganese and applying all the three afore-said methods.The least aberrations among the stated methods in defining the kinetic parameters wereobtained by using the manganese catalyst.

  14. Influence of Two-phase Ejector on Performance of Compression/Ejection rRefrigeration System%两相喷射器对压缩-喷射制冷系统性能的影响研究

    Institute of Scientific and Technical Information of China (English)

    魏新利; 汤本凯; 马新灵; 孟祥睿

    2014-01-01

    采用等面积混合模型,以 R134a 制冷剂为工质,对两相喷射器建立热力学模型,并用 Matlab7.1软件进行编程计算,相关的工质热物性参数,通过调用Refprop7软件获取。分析比较了各混合压力下喷射器内压力变化趋势,喷射器混合压力、系统蒸发温度、冷凝温度以及喷射器等熵效率的变化对两相喷射器性能、制冷系统性能的影响。结果表明:对于所研究的工质和工况,热力学分析方法采用等面积混合模型比较合理;混合压力在理论取值范围内存在一个最优点;随蒸发温度的升高或冷凝温度的降低,喷射器最优混合压力的取值点越靠近引射压力,喷射系数增加,系统COP升高,但是相对于传统压缩制冷循环的性能提高率减小;喷射器及系统性能对喷射器进口段等熵效率的变化较敏感。因而,选取恰当的混合压力值,设计制造等熵效率较高的工作喷嘴对于压缩-喷射制冷系统的性能优化至关重要。%This paper establishes a thermodynamic model of two-phase ejector, having the mixing area in the mixing chamber constant and R134a refrigerant as working medium. Matlab7.1 is used in programming and calculating. Thermo-physical properties of the refrigerant involved are obtained through application of Refprop7. Pressure changes in the ejector at various mixing pressures are analyzed. How changes in mixing pressure, evaporation temperature, condensation temperature and isentropic efficiency can influence the performance of the ejector and the refrigeration system is studied. The results show that under the operational conditions in this study, the constant area model for mixing chamber is appropriate for thermodynamic analysis. Within the theoretical value range, there is an optimum value for mixing pressure. As the evaporation temperature increases or the condensation temperature decreases, the optimum mixing pressure comes closer to

  15. Vapor Compressor Driven Hybrid Two-Phase Loop Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This Small Business Innovation Research Phase I project will demonstrate a vapor compressor driven hybrid two-phase loop technology. The hybrid two-phase loop...

  16. Experimental and numerical investigation on two-phase flow instabilities

    Energy Technology Data Exchange (ETDEWEB)

    Ruspini, Leonardo Carlos

    2013-03-01

    Two-phase flow instabilities are experimentally and numerically studied within this thesis. In particular, the phenomena called Ledinegg instability, density wave oscillations and pressure drop oscillations are investigated. The most important investigations regarding the occurrence of two-phase flow instabilities are reviewed. An extensive description of the main contributions in the experimental and analytical research is presented. In addition, a critical discussion and recommendations for future investigations are presented. A numerical framework using a hp-adaptive method is developed in order to solve the conservation equations modelling general thermo-hydraulic systems. A natural convection problem is analysed numerically in order to test the numerical solver. Moreover, the description of an adaptive strategy to solve thermo-hydraulic problems is presented. In the second part of this dissertation, a homogeneous model is used to study Ledinegg, density wave and pressure drop oscillations phenomena numerically. The dynamic characteristics of the Ledinegg (flow excursion) phenomenon are analysed through the simulation of several transient examples. In addition, density wave instabilities in boiling and condensing systems are investigated. The effects of several parameters, such as the fluid inertia and compressibility volumes, on the stability limits of Ledinegg and density wave instabilities are studied, showing a strong influence of these parameters. Moreover, the phenomenon called pressure drop oscillations is numerically investigated. A discussion of the physical representation of several models is presented with reference to the obtained numerical results. Finally, the influence of different parameters on these phenomena is analysed. In the last part, an experimental investigation of these phenomena is presented. The designing methodology used for the construction of the experimental facility is described. Several simulations and a non

  17. Assessment of fructooligosaccharides production from sucrose in aqueous and aqueous-organic systems using immobilized inulinase from Kluyveromyces marxianus NRRL Y-7571

    Directory of Open Access Journals (Sweden)

    Fernanda Vaz Alves Risso

    2012-06-01

    Full Text Available This work investigated the fructooligosaccharides (FOS synthesis by immobilized inulinase obtained from Kluyveromyces marxianus NRRL Y-7571 in aqueous and aqueous-organic systems using sucrose as substrate. The sequential strategy of experimental design was used to optimize the FOS conversion in both systems. For the aqueous-organic system, a 2(6-2 fractional design was carried out to evaluate the effects of temperature, sucrose concentration, pH, aqueous/organic ratio, enzyme activity, and polyethylene glycol concentration. For the aqueous system, a central composite design for the enzyme activity and the sucrose concentration was carried out. The highest fructooligosaccharides yield (Y FOS for the aqueous-organic system was 18.2 ± S0.9 wt%, at 40 ºC, pH 5.0, sucrose concentration of 60% (w/w, enzyme activity of 4 U.mL-1, and aqueous/organic ratio of 25/75 wt%. The highest Y FOS for the aqueous system was 14.6 ± 0.9 wt% at 40 ºC, pH 5.0, sucrose concentration of 60 wt%, and enzyme activity of 4.0 U.mL-1.

  18. Reynolds transport theorem for a two-phase flow

    Science.gov (United States)

    Collado, Francisco J.

    2007-01-01

    Transport equations for one-dimensional (1d), steady, two-phase flow have been proposed based on the fact that if the phases have different velocities, they cannot cover the same distance (the control volume length) in the same time. Thus, working in the same control volume for the two phases, the time scales of the phases have to be different. From this approach, transport balances for 1D, steady, two-phase flow have been already derived, supplying acceptable correlations for two-phase flow. Here, based on the strict application of the Reynolds transport theorem, general transport balances for two-phase flow are suggested.

  19. Experimental study of two-phase natural circulation circuit

    Energy Technology Data Exchange (ETDEWEB)

    Lemos, Wanderley Freitas; Su, Jian, E-mail: wlemos@lasme.coppe.ufrj.br, E-mail: sujian@nuclear.ufrj.br [Coordenacao dos Programas de Pos-Graduacao em Engenharia (COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear; Faccini, Jose Luiz Horacio, E-mail: faccini@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), RIo de Janeiro, RJ (Brazil). Lab. de Termo-Hidraulica Experimental

    2012-07-01

    This paper reports an experimental study on the behavior of fluid flow in natural circulation under single-and two-phase flow conditions. The natural circulation circuit was designed based on concepts of similarity and scale in proportion to the actual operating conditions of a nuclear reactor. This test equipment has similar performance to the passive system for removal of residual heat presents in Advanced Pressurized Water Reactors (A PWR). The experiment was carried out by supplying water to primary and secondary circuits, as well as electrical power resistors installed inside the heater. Power controller has available to adjust the values for supply of electrical power resistors, in order to simulate conditions of decay of power from the nuclear reactor in steady state. Data acquisition system allows the measurement and control of the temperature at different points by means of thermocouples installed at several points along the circuit. The behavior of the phenomenon of natural circulation was monitored by a software with graphical interface, showing the evolution of temperature measurement points and the results stored in digital format spreadsheets. Besides, the natural circulation flow rate was measured by a flowmeter installed on the hot leg. A flow visualization technique was used the for identifying vertical flow regimes of two-phase natural circulation. Finally, the Reynolds Number was calculated for the establishment of a friction factor correlation dependent on the scale geometrical length, height and diameter of the pipe. (author)

  20. A real two-phase submarine debris flow and tsunami

    Energy Technology Data Exchange (ETDEWEB)

    Pudasaini, Shiva P.; Miller, Stephen A. [Department of Geodynamics and Geophysics, Steinmann Institute, University of Bonn Nussallee 8, D-53115, Bonn (Germany)

    2012-09-26

    The general two-phase debris flow model proposed by Pudasaini is employed to study subaerial and submarine debris flows, and the tsunami generated by the debris impact at lakes and oceans. The model, which includes three fundamentally new and dominant physical aspects such as enhanced viscous stress, virtual mass, and generalized drag (in addition to buoyancy), constitutes the most generalized two-phase flow model to date. The advantage of this two-phase debris flow model over classical single-phase, or quasi-two-phase models, is that the initial mass can be divided into several parts by appropriately considering the solid volume fraction. These parts include a dry (landslide or rock slide), a fluid (water or muddy water; e.g., dams, rivers), and a general debris mixture material as needed in real flow simulations. This innovative formulation provides an opportunity, within a single framework, to simultaneously simulate the sliding debris (or landslide), the water lake or ocean, the debris impact at the lake or ocean, the tsunami generation and propagation, the mixing and separation between the solid and fluid phases, and the sediment transport and deposition process in the bathymetric surface. Applications of this model include (a) sediment transport on hill slopes, river streams, hydraulic channels (e.g., hydropower dams and plants); lakes, fjords, coastal lines, and aquatic ecology; and (b) submarine debris impact and the rupture of fiber optic, submarine cables and pipelines along the ocean floor, and damage to offshore drilling platforms. Numerical simulations reveal that the dynamics of debris impact induced tsunamis in mountain lakes or oceans are fundamentally different than the tsunami generated by pure rock avalanches and landslides. The analysis includes the generation, amplification and propagation of super tsunami waves and run-ups along coastlines, debris slide and deposition at the bottom floor, and debris shock waves. It is observed that the

  1. Study on Two-Phase Flow in Heterogeneous Porous Media by Light Transmission Method

    Science.gov (United States)

    Qiao, W.

    2015-12-01

    The non-aqueous phase liquid (NAPL) released to the subsurface can form residual ganglia and globules occupying pores and also accumulate and form pools, in which multiphase system forms. Determining transient fluid saturations in a multiphase system is essential to understand the flow characteristics of systems and to perform effective remediation strategies. As a non-destructive and non-invasive laboratory technique utilized for the measurement of liquid saturation in porous media, light transmission is of the lowest cost and safe. Utilization of Coupled Charge Device camera in light transmission systems provides a nearly instantaneous high-density array of spatial measurements over a very large dynamic range. The migration of NAPL and air spariging technique applied to remove NAPL in aquifer systems are typically two-phase flow problem. Because of the natural aquifer normally being heterogeneous, two 2-D sandboxes (Length55cm×width1.3cm×hight45cm) are set up to study the migration of gas and DNAPL in heterogeneous porous media based on light transmission method and its application in two-phase flow. Model D for water/gas system developed by Niemet and Selker (2001) and Model NW-A for water/NAPL system developed by Zhang et al. (2014) are applied for the calculation of fluid saturation in the two experiments, respectively. The gas injection experiments show that the gas moves upward in the irregular channels, piling up beneath the low permeability lenses and starting lateral movement. Bypassing the lenses, the gas moves upward and forms continuous distribution in the top of the sandbox. The faster of gas injects, the wider of gas migration will be. The DNAPL infiltration experiment shows that TCE mainly moves downward as the influence of gravity, stopping vertical infiltration when reaching the low permeability lenses because of its failure to overcome the capillary pressure. Then, TCE accumulates on the surface and starts transverse movement. Bypassing the

  2. SINGLE-PHASE AND TWO-PHASE SECONDARY COOLANTS: SIMULATION AND EVALUATION OF THEIR THERMOPHYSICAL PROPERTIES

    Directory of Open Access Journals (Sweden)

    Pedro Samuel Gomes Medeiros

    2011-09-01

    Full Text Available This paper makes a comparative analysis of the thermophysical properties of ice slurry with conventional single-phase secondary fluids used in thermal storage cooling systems. The ice slurry is a two-phase fluid consisting of water, antifreeze and ice crystals. It is a new technology that has shown great energy potential. In addition to transporting energy as a heat transfer fluid, it has thermal storage properties due to the presence of ice, storing coolness by latent heat of fusion. The single-phase fluids analyzed are water-NaCl and water-propylene glycol solutions, which also operate as carrier fluids in ice slurry. The presence of ice changes the thermophysical properties of aqueous solutions and a number of these properties were determined: density, thermal conductivity and dynamic viscosity. Data were obtained by software simulation. The results show that the presence of 10% by weight of ice provides a significant increase in thermal conductivity and dynamic viscosity, without causing changes in density. The rheological behavior of ice slurries, associated with its high viscosity, requires higher pumping power; however, this was not significant because higher thermal conductivity allows a lower mass flow rate without the use of larger pumps. Thus, the ice slurry ensures its high potential as a secondary fluid in thermal storage cooling systems, proving to be more efficient than single-phase secondary fluids.

  3. Flooding in counter-current two-phase flow

    Energy Technology Data Exchange (ETDEWEB)

    Ragland, W.A.; Ganic, E.N.

    1982-01-01

    Flooding is a phenomenon which is best described as the transition from counter-current to co-current flow. Early notice was taken of this phenomenon in the chemical engineering industry. Flooding also plays an important role in the field of two-phase heat transfer since it is a limit for many systems involving counter-current flow. Practical applications of flooding limited processes include wickless thermosyphons and the emergency core cooling system (ECCS) of pressurized water nuclear reactors. The phenomenon of flooding also is involved in the behavior of nuclear reactor core materials during severe accident conditions where flooding is one of the mechanisms governing the motion of the molten fuel pin cladding.

  4. Treatment of substituted phenol mixtures in single phase and two-phase solid-liquid partitioning bioreactors

    Energy Technology Data Exchange (ETDEWEB)

    Tomei, M. Concetta, E-mail: tomei@irsa.cnr.it [Water Research Institute, C.N.R., Via Salaria km 29.300, 00015 Monterotondo Scalo (Rome) (Italy); Rita, Sara; Angelucci, Domenica Mosca [Water Research Institute, C.N.R., Via Salaria km 29.300, 00015 Monterotondo Scalo (Rome) (Italy); Annesini, M. Cristina [Department of Chemical Engineering Materials and Environment, Sapienza University of Rome, Via Eudossiana 18, 00184 Rome (Italy); Daugulis, Andrew J. [Department of Chemical Engineering, Queen' s University, Kingston, Ontario, K7L 3N6 (Canada)

    2011-07-15

    Highlights: {yields} We investigate the biodegradation of a phenolic mixture (2,4-dimethylphenol and 4-nitrophenol). {yields} We identify an effective polymer to absorb these substrates. {yields} We utilize the polymer in a Two Phase Partitioning Bioreactor (TPPB) to overcome cytotoxicity. {yields} The polymer-based TPPB significantly out-performs a single phase system. {yields} The re-release of the substrates at differing rates (based on the partition coefficients) affects the process kinetics. - Abstract: The biological treatment of phenolics is constrained by the inherent cytotoxicity of these compounds. One method to alleviate such toxicity is to add a sequestering phase to absorb, and subsequently release, the substrate(s) to the micro-organisms; such a system is termed a Two Phase Partitioning Bioreactor. Here we have compared the performance of a TPPB, relative to single phase operation, in which a small volume (5%, v/v) of beads of the polymer Hytrel 8206 was used to treat aqueous mixtures of 2,4-dimethylphenol and 4-nitrophenol. Hytrel 8206 was selected from a range of polymers that were tested for their partition coefficients (PCs) for the target molecules, with the more hydrophobic compound (2,4-dimethylphenol) having a higher PC value (201) than 4-nitrophenol (143). Significantly increased removal rates for both substrates were demonstrated in TPPB mode relative to single phase operation. Additionally, the differential release of the compounds to the aqueous phase and their distinct PC values changed the kinetic pattern of the biotreatment system, smoothing out the cellular oxygen demand. Release of the substrates by the polymer over 60 operating cycles was virtually complete (>97%) demonstrating the reusability and robustness of the use of polymers in overcoming cytotoxicity of phenolic substrates.

  5. Design and experiment of household biogas system in alpine region based on two-phase anaerobic fermentation%高寒地区两相厌氧户用沼气系统设计与试验

    Institute of Scientific and Technical Information of China (English)

    孙勇; 李文哲; 张鸿琼

    2012-01-01

    摘要;针对高寒地区农村户用沼气发酵周期过长、产气量少的状况,该文提出并设计了适用于高寒地区农村户用的两相厌氧发酵装置,通过确立合理的工艺方案,控制两相厌氧发酵中产酸相、料液温度和pH值、水力滞留时间等因素,探讨了在哈尔滨地区最寒冷季节,所设计的新型农村户用沼气发酵装置产气量与维持发酵罐体内料液温度所消耗沼气量之间的关系.结果表明:基于两相厌氧发酵的高寒地区高效户用沼气发酵装置最高产气率为1.35m3/(m3·d),是传统沼气池产气率( 0.35 m3/(m3·d))的4倍.冬季1月份日均结余4.08 m3沼气量足以满足三口之家照明、炊事所需能源,实现高寒地区户用沼气全年正能输出,可以取代传统沼气池.该文研究成果为今后寒地户用沼气的发展提供技术支持和参考依据.%According to the problems of long fermentation period and low gas production in the alpine region, a two-phase anaerobic fermentation device was designed for rural household biogas production in the alpine region. The fermentation parameters such as acid production, temperature and pH value of feed and hydraulic retention time were controlled during two-phase anaerobic fermentation. The relationship between the quantity of produced biogas during fermentation and the quantity of consumed biogas to maintain the biogas slurry temperature in most cold season in Harbin was discussed. Results showed that the highest methan production was 1.35 m3/(m3d) using the two-phase anaerobic fermentation process, which was four times higher than the traditional process of 0.35 m3/(m3-d). There was 4.08 m3 daily balance biogas to satisfy lighting and cooking for three people family in January of winter, which could operate in alpine region year-round. In addition, the equipment structure is reasonable and perfect, which might replace the traditional digester. The research can provide a technical support

  6. Thirty-two phase sequences design with good autocorrelation properties

    Indian Academy of Sciences (India)

    S P Singh; K Subba Rao

    2010-02-01

    Polyphase Barker Sequences are finite length, uniform complex sequences; the magnitude of their aperiodic autocorrelation sidelobes are bounded by 1. Such sequences have been used in numerous real-world applications such as channel estimation, radar and spread spectrum communication. In this paper, thirty-two phase Barker sequences up to length 24 with an alphabet size of only 32 are presented. The sequences from length 25 to 289 have autocorrelation properties better than well-known Frank codes. Because of the complex structure the sequences are very difficult to detect and analyse by an enemy’s electronic support measures (ESMs). The synthesized sequences are promising for practical application to radar and spread spectrum communication systems. These sequences are found using the Modified Simulated Annealing Algorithm (MSAA). The convergence rate of the algorithm is good.

  7. Stability of stratified two-phase flows in horizontal channels

    CERN Document Server

    Barmak, Ilya; Ullmann, Amos; Brauner, Neima; Vitoshkin, Helen

    2016-01-01

    Linear stability of stratified two-phase flows in horizontal channels to arbitrary wavenumber disturbances is studied. The problem is reduced to Orr-Sommerfeld equations for the stream function disturbances, defined in each sublayer and coupled via boundary conditions that account also for possible interface deformation and capillary forces. Applying the Chebyshev collocation method, the equations and interface boundary conditions are reduced to the generalized eigenvalue problems solved by standard means of numerical linear algebra for the entire spectrum of eigenvalues and the associated eigenvectors. Some additional conclusions concerning the instability nature are derived from the most unstable perturbation patterns. The results are summarized in the form of stability maps showing the operational conditions at which a stratified-smooth flow pattern is stable. It is found that for gas-liquid and liquid-liquid systems the stratified flow with smooth interface is stable only in confined zone of relatively lo...

  8. Equations of two-phase flow in spray chamber

    Institute of Scientific and Technical Information of China (English)

    李新禹; 张志红; 金星; 徐杰

    2009-01-01

    The downstream water-air heat and moisture transfer system in a moving coordinate was studied. The relationship between the diameter of the misted droplets and the spray pressure was determined. Based on the theory of the relative velocity,the two-phase flow mode of the spray chamber and the efficiency equation for heat and moisture exchange were established. Corrections were carried out for the efficiency equation with spray pressure of 157 kPa. The results show that the pressure plays an important part in determining the efficiency of heat and moisture exchange. When the spray pressure is less than 157 kPa,better coincidence is noticed between the theoretical analysis and the test results with the error less than 6%. Greater error will be resulted in the case when the spray pressure is beyond 157 kPa. After the correction treatment,the coincidence between the theoretical and the experimental results is greatly improved.

  9. Phase appearance or disappearance in two-phase flows

    CERN Document Server

    Cordier, Floraine; Kumbaro, Anela

    2011-01-01

    This paper is devoted to the treatment of specific numerical problems which appear when phase appearance or disappearance occurs in models of two-phase flows. Such models have crucial importance in many industrial areas such as nuclear power plant safety studies. In this paper, two outstanding problems are identified: first, the loss of hyperbolicity of the system when a phase appears or disappears and second, the lack of positivity of standard shock capturing schemes such as the Roe scheme. After an asymptotic study of the model, this paper proposes accurate and robust numerical methods adapted to the simulation of phase appearance or disappearance. Polynomial solvers are developed to avoid the use of eigenvectors which are needed in usual shock capturing schemes, and a method based on an adaptive numerical diffusion is designed to treat the positivity problems. An alternate method, based on the use of the hyperbolic tangent function instead of a polynomial, is also considered. Numerical results are presente...

  10. Emerging Two-Phase Cooling Technologies for Power Electronic Inverters

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, J.S.

    2005-08-17

    In order to meet the Department of Energy's (DOE's) FreedomCAR and Vehicle Technologies (FVCT) goals for volume, weight, efficiency, reliability, and cost, the cooling of the power electronic devices, traction motors, and generators is critical. Currently the power electronic devices, traction motors, and generators in a hybrid electric vehicle (HEV) are primarily cooled by water-ethylene glycol (WEG) mixture. The cooling fluid operates as a single-phase coolant as the liquid phase of the WEG does not change to its vapor phase during the cooling process. In these single-phase systems, two cooling loops of WEG produce a low temperature (around 70 C) cooling loop for the power electronics and motor/generator, and higher temperature loop (around 105 C) for the internal combustion engine. There is another coolant option currently available in automobiles. It is possible to use the transmission oil as a coolant. The oil temperature exists at approximately 85 C which can be utilized to cool the power electronic and electrical devices. Because heat flux is proportional to the temperature difference between the device's hot surface and the coolant, a device that can tolerate higher temperatures enables the device to be smaller while dissipating the same amount of heat. Presently, new silicon carbide (SiC) devices and high temperature direct current (dc)-link capacitors, such as Teflon capacitors, are available but at significantly higher costs. Higher junction temperature (175 C) silicon (Si) dies are gradually emerging in the market, which will eventually help to lower hardware costs for cooling. The development of high-temperature devices is not the only way to reduce device size. Two-phase cooling that utilizes the vaporization of the liquid to dissipate heat is expected to be a very effective cooling method. Among two-phase cooling methods, different technologies such as spray, jet impingement, pool boiling and submersion, etc. are being developed. The

  11. Correct numerical simulation of a two-phase coolant

    Science.gov (United States)

    Kroshilin, A. E.; Kroshilin, V. E.

    2016-02-01

    Different models used in calculating flows of a two-phase coolant are analyzed. A system of differential equations describing the flow is presented; the hyperbolicity and stability of stationary solutions of the system is studied. The correctness of the Cauchy problem is considered. The models' ability to describe the following flows is analyzed: stable bubble and gas-droplet flows; stable flow with a level such that the bubble and gas-droplet flows are observed under and above it, respectively; and propagation of a perturbation of the phase concentration for the bubble and gas-droplet media. The solution of the problem about the breakdown of an arbitrary discontinuity has been constructed. Characteristic times of the development of an instability at different parameters of the flow are presented. Conditions at which the instability does not make it possible to perform the calculation are determined. The Riemann invariants for the nonlinear problem under consideration have been constructed. Numerical calculations have been performed for different conditions. The influence of viscosity on the structure of the discontinuity front is studied. Advantages of divergent equations are demonstrated. It is proven that a model used in almost all known investigating thermohydraulic programs, both in Russia and abroad, has significant disadvantages; in particular, it can lead to unstable solutions, which makes it necessary to introduce smoothing mechanisms and a very small step for describing regimes with a level. This does not allow one to use efficient numerical schemes for calculating the flow of two-phase currents. A possible model free from the abovementioned disadvantages is proposed.

  12. Inhibition Effect of Mace Extract Microemulsion on Vitamin C Photooxidation in Aqueous Systems

    Directory of Open Access Journals (Sweden)

    Hasbullah Hasbullah

    2014-01-01

    Full Text Available Photooxidation in food systems cause nutritional losses and produces undesirable flavor, toxic and color compounds, which make foods less acceptable or unacceptable to consumers. The objective of this research was to know the effectiveness of mace extract microemulsion to inhibit vitamin C photooxidation in aqueous systems. Aqueous food systems used are both beverage model system and apple juice beverage, where in each system enriched by 100 ppm vitamin C as substrate and 20 ppm erytrosin as photosensitiser. It is about one percent and two percent of microemulsion that contain mace extract of 0, 500 and 750 ppm were added into each of aqueous food system. Inhibition effect of mace extract microemulsion toward vitamin C photooxidation based on the rate of vitamin C degradation in aqueous food systems that illuminated by fluorescent light with 2000 lux intensity within eight hours. The result indicated the mace extract microemulsion has anti-photooxidation activity and ability to inhibit vitamin C photooxidation in aqueous systems.

  13. Radiation formation of colloidal silver particles in aqueous systems.

    Science.gov (United States)

    Cuba, Václav; Nemec, Mojmír; Gbur, Tomás; John, Jan; Pospísil, Milan; Múcka, Viliam

    2010-01-01

    This paper reports on the formation of silver nanoparticles initiated by gamma and UV radiation in various aqueous solutions. Inorganic precursors were used for radiation and/or photochemical reduction of Ag(+) ions to a metallic form. The influence of various parameters on the nucleation and formation of colloid particles was studied. Attention was also focused on the composition of the irradiated solution. Aliphatic alcohols were used as scavengers of OH radicals and other oxidizing species. The influence of the stabilizers on the formation and stability of the nanoparticles was studied.

  14. Two Phases of Coherent Structure Motions in Turbulent Boundary Layer

    Institute of Scientific and Technical Information of China (English)

    LIU Jian-Hua; JIANG Nan

    2007-01-01

    Two phases of coherent structure motion are acquired after obtaining conditional phase-averaged waveforms for longitudinal velocity of coherent structures in turbulent boundary layer based on Harr wavelet transfer. The correspondences of the two phases to the two processes (i.e. ejection and sweep) during a burst are determined.

  15. Two-phase anaerobic digestion of vegetable market waste fraction of municipal solid waste and development of improved technology for phase separation in two-phase reactor.

    Science.gov (United States)

    Majhi, Bijoy Kumar; Jash, Tushar

    2016-12-01

    Biogas production from vegetable market waste (VMW) fraction of municipal solid waste (MSW) by two-phase anaerobic digestion system should be preferred over the single-stage reactors. This is because VMW undergoes rapid acidification leading to accumulation of volatile fatty acids and consequent low pH resulting in frequent failure of digesters. The weakest part in the two-phase anaerobic reactors was the techniques applied for solid-liquid phase separation of digestate in the first reactor where solubilization, hydrolysis and acidogenesis of solid organic waste occur. In this study, a two-phase reactor which consisted of a solid-phase reactor and a methane reactor was designed, built and operated with VMW fraction of Indian MSW. A robust type filter, which is unique in its implementation method, was developed and incorporated in the solid-phase reactor to separate the process liquid produced in the first reactor. Experiments were carried out to assess the long term performance of the two-phase reactor with respect to biogas production, volatile solids reduction, pH and number of occurrence of clogging in the filtering system or choking in the process liquid transfer line. The system performed well and was operated successfully without the occurrence of clogging or any other disruptions throughout. Biogas production of 0.86-0.889m(3)kg(-1)VS, at OLR of 1.11-1.585kgm(-3)d(-1), were obtained from vegetable market waste, which were higher than the results reported for similar substrates digested in two-phase reactors. The VS reduction was 82-86%. The two-phase anaerobic digestion system was demonstrated to be stable and suitable for the treatment of VMW fraction of MSW for energy generation.

  16. Research of Characteristics of Gas-liquid Two-phase Pressure Drop in Microreactor

    Directory of Open Access Journals (Sweden)

    Li Dan

    2015-01-01

    Full Text Available With the research system of nitrogen and deionized water, this paper researches the pressure drop of gas-liquid two-phase flow in the circular microchannel with an inner diameter which is respectively 0.9mm and 0.5mm, analyzes the effect of microchannel diameter on gas-liquid two-phase frictional pressure drop in the microchannel reactor, and compares with the result of frictional pressure drop and the predicting result of divided-phase flow pattern. The result shows that, the gas-liquid two-phase frictional pressure drop in the microchannel significantly increases with the decreasing microchannel diameter; Lockhart-Martinelli relationship in divided-phase flow pattern can preferably predict the gas-liquid two-phase frictional pressure drop in the microchannel, but the Tabular constant needs to be corrected.

  17. Experimental study on transient behavior of semi-open two-phase thermosyphon

    Institute of Scientific and Technical Information of China (English)

    朱华; 王建新; 张巧惠; 屠传经

    2004-01-01

    An experimental system was set up to measure the temperature, pressure, heat transfer rate and mass flow rate in a semi-open two-phase thermosyphon. The behaviors of a semi-open two-phase thermosyphon during startup, shutdown and lack of water were studied to get complete understanding of its thermal characteristics. The variation of wall temperature, heat-exchange condition and pressure fluctuations of semi-open two-phase thermosyphons showed that the startup of SOTPT needs about 60-70 min; the startup speed of SOTPT is determined by the startup speed of the condensation section; the average pressure in the heat pipe is equal to the environmental pressure usually; the shutdown of SOTPT needs about 30-50min; a semi-open two-phase thermosyphon has good response to lack of water accident.

  18. A Rotational Pressure-Correction Scheme for Incompressible Two-Phase Flows with Open Boundaries.

    Science.gov (United States)

    Dong, S; Wang, X

    2016-01-01

    Two-phase outflows refer to situations where the interface formed between two immiscible incompressible fluids passes through open portions of the domain boundary. We present several new forms of open boundary conditions for two-phase outflow simulations within the phase field framework, as well as a rotational pressure correction based algorithm for numerically treating these open boundary conditions. Our algorithm gives rise to linear algebraic systems for the velocity and the pressure that involve only constant and time-independent coefficient matrices after discretization, despite the variable density and variable viscosity of the two-phase mixture. By comparing simulation results with theory and the experimental data, we show that the method produces physically accurate results. We also present numerical experiments to demonstrate the long-term stability of the method in situations where large density contrast, large viscosity contrast, and backflows occur at the two-phase open boundaries.

  19. New results in gravity dependent two-phase flow regime mapping

    Science.gov (United States)

    Kurwitz, Cable; Best, Frederick

    2002-01-01

    Accurate prediction of thermal-hydraulic parameters, such as the spatial gas/liquid orientation or flow regime, is required for implementation of two-phase systems. Although many flow regime transition models exist, accurate determination of both annular and slug regime boundaries is not well defined especially at lower flow rates. Furthermore, models typically indicate the regime as a sharp transition where data may indicate a transition space. Texas A&M has flown in excess of 35 flights aboard the NASA KC-135 aircraft with a unique two-phase package. These flights have produced a significant database of gravity dependent two-phase data including visual observations for flow regime identification. Two-phase flow tests conducted during recent zero-g flights have added to the flow regime database and are shown in this paper with comparisons to selected transition models. .

  20. Tunable two-phase coexistence in half-doped manganites

    Indian Academy of Sciences (India)

    P Chaddah; A Banerjee

    2008-02-01

    We discuss our very interesting experimental observation that the low-temperature two-phase coexistence in half-doped manganites is multi-valued (at any field) in that we can tune the coexisting antiferromagnetic-insulating (AF-I) and the ferromagnetic-metallic (FM-M) phase fractions by following different paths in (; ) space. We have shown experimentally that the phase fraction, in this two-phase coexistence, can take continuous infinity of values. All but one of these are metastable, and two-phase coexistence is not an equilibrium state.

  1. Passive Two-Phase Cooling of Automotive Power Electronics: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Moreno, G.; Jeffers, J. R.; Narumanchi, S.; Bennion, K.

    2014-08-01

    Experiments were conducted to evaluate the use of a passive two-phase cooling strategy as a means of cooling automotive power electronics. The proposed cooling approach utilizes an indirect cooling configuration to alleviate some reliability concerns and to allow the use of conventional power modules. An inverter-scale proof-of-concept cooling system was fabricated, and tests were conducted using the refrigerants hydrofluoroolefin HFO-1234yf and hydrofluorocarbon HFC-245fa. Results demonstrated that the system can dissipate at least 3.5 kW of heat with 250 cm3 of HFC-245fa. An advanced evaporator design that incorporates features to improve performance and reduce size was conceived. Simulation results indicate its thermal resistance can be 37% to 48% lower than automotive dual side cooled power modules. Tests were also conducted to measure the thermal performance of two air-cooled condensers--plain and rifled finned tube designs. The results combined with some analysis were then used to estimate the required condenser size per operating conditions and maximum allowable system (i.e., vapor and liquid) temperatures.

  2. IMPROVED SUBGRID SCALE MODEL FOR DENSE TURBULENT SOLID-LIQUID TWO-PHASE FLOWS

    Institute of Scientific and Technical Information of China (English)

    TANG Xuelin; QIAN Zhongdong; WU Yulin

    2004-01-01

    The dense solid-phase governing equations for two-phase flows are obtained by using the kinetic theory of gas molecules. Assuming that the solid-phase velocity distributions obey the Maxwell equations, the collision term for particles under dense two-phase flow conditions is also derived.In comparison with the governing equations of a dilute two-phase flow, the solid-particle's governing equations are developed for a dense turbulent solid-liquid flow by adopting some relevant terms from the dilute two-phase governing equations. Based on Cauchy-Helmholtz theorem and Smagorinsky model,a second-order dynamic sub-grid-scale (SGS) model, in which the sub-grid-scale stress is a function of both the strain-rate tensor and the rotation-rate tensor, is proposed to model the two-phase governing equations by applying dimension analyses. Applying the SIMPLEC algorithm and staggering grid system to the two-phase discretized governing equations and employing the slip boundary conditions on the walls, the velocity and pressure fields, and the volumetric concentration are calculated. The simulation results are in a fairly good agreement with experimental data in two operating cases in a conduit with a rectangular cross-section and these comparisons imply that these models are practical.

  3. Mixed Model for Silt-Laden Solid-Liquid Two-Phase Flows

    Institute of Scientific and Technical Information of China (English)

    唐学林; 徐宇; 吴玉林

    2003-01-01

    The kinetic theory of molecular gases was used to derive the governing equations for dense solid-liquid two-phase flows from a microscopic flow characteristics viewpoint by multiplying the Boltzmann equation for each phase by property parameters and integrating over the velocity space. The particle collision term was derived from microscopic terms by comparison with dilute two-phase flow but with consideration of the collisions between particles for dense two-phase flow conditions and by assuming that the particle-phase velocity distribution obeys the Maxwell equations. Appropriate terms from the dilute two-phase governing equations were combined with the dense particle collision term to develop the governing equations for dense solid-liquid turbulent flows. The SIMPLEC algorithm and a staggered grid system were used to solve the discretized two-phase governing equations with a Reynolds averaged turbulence model. Dense solid-liquid turbulent two-phase flows were simulated for flow in a duct. The simulation results agree well with experimental data.

  4. Particle modulations to turbulence in two-phase round jets

    Institute of Scientific and Technical Information of China (English)

    Bing Wang; Huiqiang Zhang; Yi Liu; Xiaofen Yan; Xilin Wang

    2009-01-01

    The particle modulations to turbulence in round jets were experimentally studied by means of two-phase velocity measurements with Phase Doppler Anemometer (PDA). Laden with very large particles, no significant attenuations of turbulence intensities were measured in the far-fields, due to small two-phase slip velocities and particle Reynolds number. The gas-phase turbulence is enhanced by particles in the near-fields, but it is significantly attenuated by the small particles in the far-fields. The smaller particles have a more profound effect on the attenuation of turbulence intensities. The enhancements or attenuations of turbulence intensities in the far-fields depends on the energy production, transport and dissipation mechanisms between the two phases, which are determined by the particle prop-erties and two-phase velocity slips. The non-dimensional parameter CTI is introduced to represent the change of turbulence intensity.

  5. Gravity Independence of Microchannel Two-Phase Flow Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Most of the amassed two-phase flow and heat transfer knowledge comes from experiments conducted in Earth’s gravity. Space missions span varying gravity levels,...

  6. Vapor Compressor Driven Hybrid Two-Phase Loop Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Phase I project successfully demonstrated the feasibility of the vapor compression hybrid two-phase loop (VCHTPL). The test results showed the high...

  7. Creep of Two-Phase Microstructures for Microelectronic Applications

    Energy Technology Data Exchange (ETDEWEB)

    Reynolds, Heidi Linch [Univ. of California, Berkeley, CA (United States)

    1998-12-01

    The mechanical properties of low-melting temperature alloys are highly influenced by their creep behavior. This study investigates the dominant mechanisms that control creep behavior of two-phase, low-melting temperature alloys as a function of microstructure. The alloy systems selected for study were In-Ag and Sn-Bi because their eutectic compositions represent distinctly different microstructure.” The In-Ag eutectic contains a discontinuous phase while the Sn-Bi eutectic consists of two continuous phases. In addition, this work generates useful engineering data on Pb-free alloys with a joint specimen geometry that simulates microstructure found in microelectronic applications. The use of joint test specimens allows for observations regarding the practical attainability of superplastic microstructure in real solder joints by varying the cooling rate. Steady-state creep properties of In-Ag eutectic, Sn-Bi eutectic, Sn-xBi solid-solution and pure Bi joints have been measured using constant load tests at temperatures ranging from O°C to 90°C. Constitutive equations are derived to describe the steady-state creep behavior for In-Ageutectic solder joints and Sn-xBi solid-solution joints. The data are well represented by an equation of the form proposed by Dom: a power-law equation applies to each independent creep mechanism. Rate-controlling creep mechanisms, as a function of applied shear stress, test temperature, and joint microstructure, are discussed. Literature data on the steady-state creep properties of Sn-Bi eutectic are reviewed and compared with the Sn-xBi solid-solution and pure Bi joint data measured in the current study. The role of constituent phases in controlling eutectic creep behavior is discussed for both alloy systems. In general, for continuous, two-phase microstructure, where each phase exhibits significantly different creep behavior, the harder or more creep resistant phase will dominate the creep behavior in a lamellar microstructure. If a

  8. Unsteady flow analysis of a two-phase hydraulic coupling

    Science.gov (United States)

    Hur, N.; Kwak, M.; Lee, W. J.; Moshfeghi, M.; Chang, C.-S.; Kang, N.-W.

    2016-06-01

    Hydraulic couplings are being widely used for torque transmitting between separate shafts. A mechanism for controlling the transmitted torque of a hydraulic system is to change the amount of working fluid inside the system. This paper numerically investigates three-dimensional turbulent flow in a real hydraulic coupling with different ratios of charged working fluid. Working fluid is assumed to be water and the Realizable k-ɛ turbulence model together with the VOF method are used to investigate two-phase flow inside the wheels. Unsteady simulations are conducted using the sliding mesh technique. The primary wheel is rotating at a fixed speed of 1780 rpm and the secondary wheel rotates at different speeds for simulating different speed ratios. Results are investigated for different blade angles, speed ratios and also different water volume fractions, and are presented in the form of flow patterns, fluid average velocity and also torques values. According to the results, blade angle severely affects the velocity vector and the transmitted torque. Also in the partially-filled cases, air is accumulated in the center of the wheel forming a toroidal shape wrapped by water and the transmitted torque sensitively depends on the water volume fraction. In addition, in the fully-filled case the transmitted torque decreases as the speed ration increases and the average velocity associated with lower speed ratios are higher.

  9. Dynamic behavior of pipes conveying gas–liquid two-phase flow

    Energy Technology Data Exchange (ETDEWEB)

    An, Chen, E-mail: anchen@cup.edu.cn [Offshore Oil/Gas Research Center, China University of Petroleum-Beijing, Beijing 102249 (China); Su, Jian, E-mail: sujian@lasme.coppe.ufrj.br [Nuclear Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, CP 68509, Rio de Janeiro 21941-972 (Brazil)

    2015-10-15

    Highlights: • Dynamic behavior of pipes conveying gas–liquid two-phase flow was analyzed. • The generalized integral transform technique (GITT) was applied. • Excellent convergence behavior and long-time stability were shown. • Effects of volumetric quality and volumetric flow rate on dynamic behavior were studied. • Normalized volumetric-flow-rate stability envelope of dynamic system was determined. - Abstract: In this paper, the dynamic behavior of pipes conveying gas–liquid two-phase flow was analytically and numerically investigated on the basis of the generalized integral transform technique (GITT). The use of the GITT approach in the analysis of the transverse vibration equation lead to a coupled system of second order differential equations in the dimensionless temporal variable. The Mathematica's built-in function, NDSolve, was employed to numerically solve the resulting transformed ODE system. The characteristics of gas–liquid two-phase flow were represented by a slip-ratio factor model that was devised and used for similar problems. Good convergence behavior of the proposed eigenfunction expansions is demonstrated for calculating the transverse displacement at various points of pipes conveying air–water two-phase flow. Parametric studies were performed to analyze the effects of the volumetric gas fraction and the volumetric flow rate on the dynamic behavior of pipes conveying air–water two-phase flow. Besides, the normalized volumetric-flow-rate stability envelope for the dynamic system was obtained.

  10. LIQUID-LIQUID EQUILIBRIA OF AQUEOUS TWO-PHASE SYSTEMS CONTAINING POLY(ETHYLENE GLYCOL)+ NANO3 OR NACIO4 OR KCIO4 AND PARTITION OF NACIO4, KCIO4 AND NANO3

    OpenAIRE

    JIMENEZ BELLOTT, YECID PONCIANO

    2012-01-01

    En la presente Tesis Doctoral se determino el equilibrio líquido-líquido de los sistemas acuosos bifásicos (SAB) Polietilenglicol (PEG 4000) + NaNO3, PEG 4000 + NaCIO4 y PEG 4000 + KCIO4 a las temperaturas de 288.15, 298.15 y 308.15 K. y la partición de NaCIO4, KCIO4 y NaN03 en los SABs antes mencionados. Las curvas binodales fueron determinadas por el método turbidimétrico y las líneas de equilibrio o "Tie lines" llevando las soluciones al equilibrio. De estas pruebas experimentales se ob...

  11. International Conference on Partitioning in Aqueous Two-Phase Systems in Biochemistry Cell Biology and Biotechnology (7th) Held in New Orleans, Louisiana on June 2-7, 1991.

    Science.gov (United States)

    1991-11-30

    PARTITIONING OF CEREBROCORTICAL SYNAPTOSOMES M. J. L6pez.P6rez Departamento de Bioquimica y Biologia Molecular y Celular . Facultad de Veterinaria...Pascual, T. Muifio-Blanco, J. A. Cebriin-Pdrez and M. J. L6pez-Pdrez. Departamento de Bioqufmica y Biologia Molecular y Celular . Facultad de...Institute, Chemistry and Life Sciences, P.O. Box 12194, Research Triangle Park, NC 27709-2194 D. C. CHEN AND P. RAY, Division of Molecular Genetic

  12. Study on Distribution Behavior of Human Serum Albumin in Aqueous Two-Phase System%人血清白蛋白在双水相法体系中的分配规律研究

    Institute of Scientific and Technical Information of China (English)

    刘俊果

    2013-01-01

    人血清白蛋白是一种重要的临床药用蛋白质.以PEG/(NH4)2SO4双水相体系萃取分离人血清白蛋白,探索人血清白蛋白在双水相体系中的分配规律.结果表明,人血清白蛋白在双水相体系中的分配系数与萃取率均随着PEG分子量的增大而增大;随PEG和(NH4)2SO4浓度的增加而增加,当PEG为19.53%、(NH4)2SO4为14.07%时达到最大;但若持续增大各相的浓度,其分配系数与萃取率又会下降;随着体系中外加盐NaC1浓度的增加而增加,在NaC1浓度为10%时达到最大.

  13. International Conference on Partitioning in Aqueous Two-Phase Systems: Advances in Separation in Biochenistry, Cell Biology and Biotechnology (7th) Held in New Orleans, Louisiana on 2-7 June 1991.

    Science.gov (United States)

    1991-06-27

    de Bioquimica y Biologia Molecular y Celular. Facultad de Veterinaria. Universidad de Zaragoza. SPAIN Homogenization of brain tissue results in nerve...School of Medicine, London, U.K. Jose Luque, Jesus Nendieta, Pilar Sancho Departamento de Bioqulmica y Biologia Molecular , Universidad de Alcal& de...and M. J. L6pez-Pirez. Departamento de Bioquimica y Biologia Molecular y Celular. Facultad de Veterinaria. Universidad de Zaragoza. SPAIN Previous

  14. 超声波协同双水相体系提取芫荽总黄酮的研究%Aqueous two-phase system and ultrasonic wave co-extraction of total flavones from coriandrum sativum

    Institute of Scientific and Technical Information of China (English)

    王顺民; 季长路; 任晶晶

    2009-01-01

    主要研究了将超声波技术和双水相萃取技术耦合用于提取芫荽中总黄酮.通过单因素实验和正交试验得出,影响芫荽总黄酮的提取因素的主次顺序为溶剂浓度>超声时间>料液比.最佳提取工艺条件为乙醇体积分数50%、料液比1∶20、超声提取20min,总黄酮得率达2.8%.

  15. Microporous silica gels from alkylsilicate-water two phase hydrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Chu, L.; Tejedor-Tejedor, M.I.; Anderson, M.A. [Univ. of Wisconsin, Madison, WI (United States). Water Chemistry Program

    1994-12-31

    Microporous silica gels have been synthesized through a nano-particulate sol-gel route. These gels have uniformly distributed and extremely small pores(< 15 {angstrom} in diameter). Hydrolysis and condensation reactions leading to these gels were carried out in an alkyl silicate-water (ammonia) two phase system. These reactions took place at the alkyl silicate droplet-water interfacial boundary. No alcohol was added. A clear, stable and uniformly distributed colloidal silica suspension having an average particle size less than 6 nm was prepared by this method. Fast hydrolysis, slow condensation and low solubility all contribute to a high supersaturation level and result in the formation of small particles. This process is consistent with classic nucleation theory. When the particles are produced under acidic rather than under basic reaction conditions, smaller particles are formed due to the slower condensation rate and lower solubility of these silica particles in acidic conditions. At the same pH, alkylsilicates having smaller alkyl groups react faster with water leading to smaller primary particles. Homogeneous nucleation conditions are achieved when the water/alkylsilicate ratio is high.

  16. Stability of stratified two-phase flows in horizontal channels

    Science.gov (United States)

    Barmak, I.; Gelfgat, A.; Vitoshkin, H.; Ullmann, A.; Brauner, N.

    2016-04-01

    Linear stability of stratified two-phase flows in horizontal channels to arbitrary wavenumber disturbances is studied. The problem is reduced to Orr-Sommerfeld equations for the stream function disturbances, defined in each sublayer and coupled via boundary conditions that account also for possible interface deformation and capillary forces. Applying the Chebyshev collocation method, the equations and interface boundary conditions are reduced to the generalized eigenvalue problems solved by standard means of numerical linear algebra for the entire spectrum of eigenvalues and the associated eigenvectors. Some additional conclusions concerning the instability nature are derived from the most unstable perturbation patterns. The results are summarized in the form of stability maps showing the operational conditions at which a stratified-smooth flow pattern is stable. It is found that for gas-liquid and liquid-liquid systems, the stratified flow with a smooth interface is stable only in confined zone of relatively low flow rates, which is in agreement with experiments, but is not predicted by long-wave analysis. Depending on the flow conditions, the critical perturbations can originate mainly at the interface (so-called "interfacial modes of instability") or in the bulk of one of the phases (i.e., "shear modes"). The present analysis revealed that there is no definite correlation between the type of instability and the perturbation wavelength.

  17. Pressure transient analysis of two-phase flow problems

    Energy Technology Data Exchange (ETDEWEB)

    Chu, W.C.; Reynolds, A.C.; Raghavan, R.

    1986-04-01

    This paper considers the analysis of pressure drawdown and buildup data for two-phase flow problems. Of primary concern is the analysis of data influenced by saturation gradients that exist within the reservoir. Wellbore storage effects are assumed to be negligible. The pressure data considered are obtained from a two-dimensional (2D) numerical coning model for an oil/water system. The authors consider constant-rate production followed by a buildup period and assume that the top, bottom, and outer boundaries of the reservoir are sealed. First, they consider the case where the producing interval is equal to the total formation thickness. Second, they discuss the effect of partial penetration. In both cases, they show that average pressure can be estimated by the Matthews-Brons-Hazebroek method and consider the computation of the skin factor. They also show that a reservoir limit test can estimate reservoir PV only if the total mobility adjacent to the wellbore does not vary with time.

  18. STUDIES OF TWO-PHASE PLUMES IN STRATIFIED ENVIRONMENTS

    Energy Technology Data Exchange (ETDEWEB)

    Scott A. Socolofsky; Brian C. Crounse; E. Eric Adams

    1998-11-18

    Two-phase plumes play an important role in the more practical scenarios for ocean sequestration of CO{sub 2}--i.e. dispersing CO{sub 2} as a buoyant liquid from either a bottom-mounted or ship-towed pipeline. Despite much research on related applications, such as for reservoir destratification using bubble plumes, our understanding of these flows is incomplete, especially concerning the phenomenon of plume peeling in a stratified ambient. To address this deficiency, we have built a laboratory facility in which we can make fundamental measurements of plume behavior. Although we are using air, oil and sediments as our sources of buoyancy (rather than CO{sub 2}), by using models, our results can be directly applied to field scale CO{sub 2} releases to help us design better CO{sub 2} injection systems, as well as plan and interpret the results of our up-coming international field experiment. The experimental facility designed to study two-phase plume behavior similar to that of an ocean CO{sub 2} release includes the following components: 1.22 x 1.22 x 2.44 m tall glass walled tank; Tanks and piping for the two-tank stratification method for producing step- and linearly-stratified ambient conditions; Density profiling system using a conductivity and temperature probe mounted to an automated depth profiler; Lighting systems, including a virtual point source light for shadowgraphs and a 6 W argon-ion laser for laser induced fluorescence (LIF) imaging; Imaging system, including a digital, progressive scanning CCD camera, computerized framegrabber, and image acquisition and analysis software; Buoyancy source diffusers having four different air diffusers, two oil diffusers, and a planned sediment diffuser; Dye injection method using a Mariotte bottle and a collar diffuser; and Systems integration software using the Labview graphical programming language and Windows NT. In comparison with previously reported experiments, this system allows us to extend the parameter range of

  19. Modeling of vapor-liquid-solid equilibrium in gas - aqueous electrolyte systems

    DEFF Research Database (Denmark)

    Thomsen, Kaj; Rasmussen, Peter

    1999-01-01

    A thermodynamic model for the description of vapor-liquid-solid equilibria is introduced. This model is a combination of the extended UNIQUAC model for electrolytes and the Soave-Redlich-Kwong cubic equation of state. The model has been applied to aqueous systems containing ammonia and/or carbon...

  20. Impact of ligand protonation on higher-order metal complexation kinetics in aqueous systems

    NARCIS (Netherlands)

    Town, R.M.; Leeuwen, van H.P.

    2008-01-01

    The impact of ligand protonation on the complexation kinetics of higher-order complexes is quantitatively described. The theory is formulated on the basis of the usual situation for metal complex formation in aqueous systems in which the exchange of water for the ligand in the inner coordination sph

  1. The leachability of carbon-14-labelled 3,4-benzopyrene from coal ash into aqueous systems

    NARCIS (Netherlands)

    Besemer, A.C.; Kanij, J.

    1984-01-01

    The leachability of polycyclic aromatic hydrocarbons from coal ash into aqueous systems was studied. Carbon-14-labeled 3,4-Benzopyrene (BaP) was deposited on coal fly ash by adsorption from the liquid phase in quantities of about 10 ??g/g ash. After a thermal treatment in air at 120??C for 2 hours t

  2. Biocompatibility of low molecular weight polymers for two-phase partitioning bioreactors.

    Science.gov (United States)

    Harris, Jesse; Daugulis, Andrew J

    2015-12-01

    Two phase partitioning bioreactors (TPPBs) improve the efficiency of fermentative processes by limiting the exposure of microorganisms to toxic solutes by sequestering them into a non-aqueous phase (NAP). A potential limitation of this technology, when using immiscible organic solvents as the NAP, is the cytoxicity that these materials may exert on the microbes. An improved TPPB configuration is one in which polymeric NAPs are used to replace organic solvents in order to take advantage of their low cost, improved handling qualities, and biocompatibility. A recent study has shown that low molecular weight polymers may confer improved solute uptake relative to high molecular weight polymers (i.e., have higher partition coefficients), but it is unknown whether sufficiently low molecular weight polymers may inhibit cell growth. This study has investigated the biocompatibility of a range of low molecular weight polymers, and compared trends in biocompatibility to the well-established "critical log P" concept. This was achieved by determining the biocompatibility of polypropylene glycol polymers over a molecular weight (MW) range of 425-4,000 to Saccharomyces cerevisiae and Pseudomonas putida, two organisms which have been previously used in TPPB systems. The lower MW polymers were shown to have lower average log P values, and showed more cytotoxicity than polymers of the same structure but with higher molecular weight. Since polymers are generally polydisperse (i.e., polymer samples contain a distribution of MWs), removal of the lower MW fractions via water washing was found to result in improved polymer biocompatibility. These results suggest that the critical log P concept remains useful for describing the toxicity of polymeric substances of different MWs, although it is complicated by the presence of the low MW fractions in the polymers arising from polydispersity.

  3. Performance of a passive emergency heat removal system of advanced reactors in two-phase flow and with high concentration of non-condensable; Atuacao de um sistema passivo de remocao de calor de emergencia de reatores avancados em escoamento bifasico e com alta concentracao de nao-condensaveis

    Energy Technology Data Exchange (ETDEWEB)

    Macedo, Luiz Alberto

    2008-07-01

    The research and the development of passive emergency cooling systems are necessary for the new generation of thermo-nuclear systems. Some basic information on the operation of these systems require the research of some relative processes to the natural circulation, mainly in conditions of two-phase flow involving processes of condensation in the presence of non-condensable gases, because many found situations are new. The experimental facility called Bancada de Circulacao Natural (BCN) was used for the realization of tests with diverse concentrations of non-condensable and power levels. The non-condensable gas present in the circuit decreases the rate of heat transfer for the secondary of the heat exchanger, determining low efficiency of the heat exchanger. High concentration of non-condensable in the vapor condensation, determines negative pressure, and cause the inversion of the flow in the circuit. The initial concentration of non-condensable and the geometry of the circuit, in the inlet of the heat exchanger, determines the establishment of transitory with two-phase flow. The BCN was performed with the computational code of Analysis of Accidents and Thermal-Hydraulics RELAP5/MOD 3.3 and, the calculated values had been compared with the experimental data, presenting good agreement for small non-condensable concentrations. The values calculated for high concentrations of non-condensable had been satisfactory after the circuit to have reached the temperature of saturation in the electric heater. (author)

  4. Characterizing dynamic hysteresis and fractal statistics of chaotic two-phase flow and application to fuel cells

    Science.gov (United States)

    Burkholder, Michael B.; Litster, Shawn

    2016-05-01

    In this study, we analyze the stability of two-phase flow regimes and their transitions using chaotic and fractal statistics, and we report new measurements of dynamic two-phase pressure drop hysteresis that is related to flow regime stability and channel water content. Two-phase flow dynamics are relevant to a variety of real-world systems, and quantifying transient two-phase flow phenomena is important for efficient design. We recorded two-phase (air and water) pressure drops and flow images in a microchannel under both steady and transient conditions. Using Lyapunov exponents and Hurst exponents to characterize the steady-state pressure fluctuations, we develop a new, measurable regime identification criteria based on the dynamic stability of the two-phase pressure signal. We also applied a new experimental technique by continuously cycling the air flow rate to study dynamic hysteresis in two-phase pressure drops, which is separate from steady-state hysteresis and can be used to understand two-phase flow development time scales. Using recorded images of the two-phase flow, we show that the capacitive dynamic hysteresis is related to channel water content and flow regime stability. The mixed-wettability microchannel and in-channel water introduction used in this study simulate a polymer electrolyte fuel cell cathode air flow channel.

  5. Investigation on two-phase flow instability in steam generator of integrated nuclear reactor

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    In the pressure range of 3-18MPa,high pressure steam-water two-phase flow density wave instability in vertical upward parallel pipes with inner diameter of 12mm is studied experimentally.The oscillation curves of two-phase flow instability and the effects of several parameters on the oscillation threshold of the system are obtained.Based on the small pertubation linearization method and the stability principles of automatic control system,a mathematical model is developed to predict the characteristics of density wave instability threshold.The predictions of the model are in good agreement with the experimental results.

  6. Determination of production-shipment policy using a two-phase algebraic approach

    Directory of Open Access Journals (Sweden)

    Huei-Hsin Chang

    2012-04-01

    Full Text Available The optimal production-shipment policy for end products using mathematicalmodeling and a two-phase algebraic approach is investigated. A manufacturing systemwith a random defective rate, a rework process, and multiple deliveries is studied with thepurpose of deriving the optimal replenishment lot size and shipment policy that minimisestotal production-delivery costs. The conventional method uses differential calculus on thesystem cost function to determine the economic lot size and optimal number of shipmentsfor such an integrated vendor-buyer system, whereas the proposed two-phase algebraicapproach is a straightforward method that enables practitioners who may not havesufficient knowledge of calculus to manage real-world systems more effectively.

  7. Comparison of two-phase and three-phase methanol synthesis processes

    NARCIS (Netherlands)

    van de Graaf, G.H; Beenackers, A.A C M

    1996-01-01

    A comparison is made between the ICI (two-phase) methanol synthesis process and a three-phase slurry process based on a multi-stage agitated reactor. The process calculations are based on a complete reactor system consisting of the reactor itself, a recycling system and a gas-liquid separator. The b

  8. New Results in Two-Phase Pressure Drop Calculations at Reduced Gravity Conditions

    Science.gov (United States)

    Braisted, Jon; Kurwitz, Cable; Best, Frederick

    2004-02-01

    The mass, power, and volume energy savings of two-phase systems for future spacecraft creates many advantages over current single-phase systems. Current models of two-phase phenomena such as pressure drop, void fraction, and flow regime prediction are still not well defined for space applications. Commercially available two-phase modeling software has been developed for a large range of acceleration fields including reduced-gravity conditions. Recently, a two-phase experiment has been flown to expand the two-phase database. A model of the experiment was created in the software to determine how well the software could predict the pressure drop observed in the experiment. Of the simulations conducted, the computer model shows good agreement of the pressure drop in the experiment to within 30%. However, the software does begin to over-predict pressure drop in certain regions of a flow regime map indicating that some models used in the software package for reduced-gravity modeling need improvement.

  9. Gas-liquid two-phase flow across a bank of micropillars

    Science.gov (United States)

    Krishnamurthy, Santosh; Peles, Yoav

    2007-04-01

    Adiabatic nitrogen-water two-phase flow across a bank of staggered circular micropillars, 100μm long with a diameter of 100μm and a pitch-to-diameter ratio of 1.5, was investigated experimentally for Reynolds number ranging from 5 to 50. Flow patterns, void fraction, and pressure drop were obtained, discussed, and compared to large scale as well as microchannel results. Two-phase flow patterns were determined by flow visualization, and a flow map was constructed as a function of gas and liquid superficial velocities. Significant deviations from conventional scale systems, with respect to flow patterns and trend lines, were observed. A unique flow pattern, driven by surface tension, was observed and termed bridge flow. The applicability of conventional scale models to predict the void fraction and two-phase frictional pressure drop was also assessed. Comparison with a conventional scale void fraction model revealed good agreement, but was found to be in a physically wrong form. Thus, a modified physically based model for void fraction was developed. A two-phase frictional multiplier was found to be a strong function of mass flux, unlike in previous microchannel studies. It was observed that models from conventional scale systems did not adequately predict the two-phase frictional multiplier at the microscale, thus, a modified model accounting for mass flux was developed.

  10. A Derivation of the Nonlocal Volume-Averaged Equations for Two-Phase Flow Transport

    Directory of Open Access Journals (Sweden)

    Gilberto Espinosa-Paredes

    2012-01-01

    Full Text Available In this paper a detailed derivation of the general transport equations for two-phase systems using a method based on nonlocal volume averaging is presented. The local volume averaging equations are commonly applied in nuclear reactor system for optimal design and safe operation. Unfortunately, these equations are limited to length-scale restriction and according with the theory of the averaging volume method, these fail in transition of the flow patterns and boundaries between two-phase flow and solid, which produce rapid changes in the physical properties and void fraction. The non-local volume averaging equations derived in this work contain new terms related with non-local transport effects due to accumulation, convection diffusion and transport properties for two-phase flow; for instance, they can be applied in the boundary between a two-phase flow and a solid phase, or in the boundary of the transition region of two-phase flows where the local volume averaging equations fail.

  11. Reduced-gravity two-phase flow experiments in the NASA KC-135

    Science.gov (United States)

    Cuta, Judith M.; Michener, Thomas E.; Best, Frederick R.; Kachnik, Leo J.

    1988-01-01

    An adequate understanding is sought of flow and heat transfer behavior in reduced and zero gravity conditions. Microgravity thermal-hydraulic analysis capabilities were developed for application to space nuclear power systems. A series of reduced gravity two phase flow experiments using the NASA KC-135 were performed. The objective was to supply basic thermal hydraulic information that could be used in development of analytical tools for design of space power systems. The experiments are described. Two main conclusions were drawn. First, the tests demonstrate that the KC-135 is a suitable test environment for obtaining two phase flow and heat transfer data in reduced gravity conditions. Second, the behavior of two phase flow in low gravity is sufficiently different from that obtained in 1 g to warrant intensive investigation of the phenomenon if adequate analytical tools are to be developed for microgravity conditions.

  12. DYNAMIC MODELING STRATEGY FOR FLOW REGIME TRANSITION IN GAS-LIQUID TWO-PHASE FLOWS

    Energy Technology Data Exchange (ETDEWEB)

    X. Wang; X. Sun; H. Zhao

    2011-09-01

    In modeling gas-liquid two-phase flows, the concept of flow regime has been used to characterize the global interfacial structure of the flows. Nearly all constitutive relations that provide closures to the interfacial transfers in two-phase flow models, such as the two-fluid model, are often flow regime dependent. Currently, the determination of the flow regimes is primarily based on flow regime maps or transition criteria, which are developed for steady-state, fully-developed flows and widely applied in nuclear reactor system safety analysis codes, such as RELAP5. As two-phase flows are observed to be dynamic in nature (fully-developed two-phase flows generally do not exist in real applications), it is of importance to model the flow regime transition dynamically for more accurate predictions of two-phase flows. The present work aims to develop a dynamic modeling strategy for determining flow regimes in gas-liquid two-phase flows through the introduction of interfacial area transport equations (IATEs) within the framework of a two-fluid model. The IATE is a transport equation that models the interfacial area concentration by considering the creation and destruction of the interfacial area, such as the fluid particle (bubble or liquid droplet) disintegration, boiling and evaporation; and fluid particle coalescence and condensation, respectively. For the flow regimes beyond bubbly flows, a two-group IATE has been proposed, in which bubbles are divided into two groups based on their size and shape (which are correlated), namely small bubbles and large bubbles. A preliminary approach to dynamically identifying the flow regimes is provided, in which discriminators are based on the predicted information, such as the void fraction and interfacial area concentration of small bubble and large bubble groups. This method is expected to be applied to computer codes to improve their predictive capabilities of gas-liquid two-phase flows, in particular for the applications in

  13. Shadow imaging in bubbly gas-liquid two-phase flow in porous structures

    Science.gov (United States)

    Altheimer, Marco; Häfeli, Richard; Wälchli, Carmen; Rudolf von Rohr, Philipp

    2015-09-01

    Shadow imaging is used for the investigation of bubbly gas-liquid two-phase flow in a porous structure. The porous structure is made of Somos WaterShed XC 11122, a clear epoxy resin used in rapid prototyping. Optical access is provided by using an aqueous solution of sodium iodide and zinc iodide having the same refractive index as the structure material (). Nitrogen is injected into the continuous phase at volumetric transport fractions in the range of resulting in a hold-up of . The obtained images of overlapping bubble shadows are processed to measure the bubble dimensions. Therefore, a new processing sequence is developed to determine bubble dimensions from overlapping bubble shadows by ellipse fitting. The accuracy of the bubble detection and sizing routine is assessed processing synthetic images. It is shown that the developed technique is suitable for volumetric two-phase flow measurements. Important global quantities such as gas hold-up and total interfacial area can be measured with only one camera. Operation parameters for gas-liquid two-phase flows are determined to improve mass and heat transfer between the phases.

  14. A mechanical erosion model for two-phase mass flows

    CERN Document Server

    Pudasaini, Shiva P

    2016-01-01

    Erosion, entrainment and deposition are complex and dominant, but yet poorly understood, mechanical processes in geophysical mass flows. Here, we propose a novel, process-based, two-phase, erosion-deposition model capable of adequately describing these complex phenomena commonly observed in landslides, avalanches, debris flows and bedload transport. The model is based on the jump in the momentum flux including changes of material and flow properties along the flow-bed interface and enhances an existing general two-phase mass flow model (Pudasaini, 2012). A two-phase variably saturated erodible basal morphology is introduced and allows for the evolution of erosion-deposition-depths, incorporating the inherent physical process including momentum and rheological changes of the flowing mixture. By rigorous derivation, we show that appropriate incorporation of the mass and momentum productions or losses in conservative model formulation is essential for the physically correct and mathematically consistent descript...

  15. Two-Phase flow instrumentation for nuclear accidents simulation

    Science.gov (United States)

    Monni, G.; De Salve, M.; Panella, B.

    2014-11-01

    The paper presents the research work performed at the Energy Department of the Politecnico di Torino, concerning the development of two-phase flow instrumentation and of models, based on the analysis of experimental data, that are able to interpret the measurement signals. The study has been performed with particular reference to the design of power plants, such as nuclear water reactors, where the two-phase flow thermal fluid dynamics must be accurately modeled and predicted. In two-phase flow typically a set of different measurement instruments (Spool Piece - SP) must be installed in order to evaluate the mass flow rate of the phases in a large range of flow conditions (flow patterns, pressures and temperatures); moreover, an interpretative model of the SP need to be developed and experimentally verified. The investigated meters are: Turbine, Venturi, Impedance Probes, Concave sensors, Wire mesh sensor, Electrical Capacitance Probe. Different instrument combinations have been tested, and the performance of each one has been analyzed.

  16. Simulating confined swirling gas-solid two phase jet

    Institute of Scientific and Technical Information of China (English)

    金晗辉; 夏钧; 樊建人; 岑可法

    2002-01-01

    A k-ε-kp multi-fluid model was used to simulate confined swirling gas-solid two phase jet comprised of particle-laden flow from a center tube and a swirling air stream entering the test section from the coaxial annular. After considering the drag force between the two phases and gravity, a series of numerical simulations of the two-phase flow of 30μm, 45μm, 60μm diameter particles were performed on a x×r=50×50 mesh grid respectively. The results showed that the k-ε-kp multi-fluid model can be applied to predict moderate swirling multi-phase flow. When the particle diameter is large, the collision of the particles with the wall will influence the prediction accuracy. The bigger the diameter of the particles, the stronger the collision with the wall, and the more obvious the difference between measured and calculated results.

  17. Mathematical modeling of disperse two-phase flows

    CERN Document Server

    Morel, Christophe

    2015-01-01

    This book develops the theoretical foundations of disperse two-phase flows, which are characterized by the existence of bubbles, droplets or solid particles finely dispersed in a carrier fluid, which can be a liquid or a gas. Chapters clarify many difficult subjects, including modeling of the interfacial area concentration. Basic knowledge of the subjects treated in this book is essential to practitioners of Computational Fluid Dynamics for two-phase flows in a variety of industrial and environmental settings. The author provides a complete derivation of the basic equations, followed by more advanced subjects like turbulence equations for the two phases (continuous and disperse) and multi-size particulate flow modeling. As well as theoretical material, readers will discover chapters concerned with closure relations and numerical issues. Many physical models are presented, covering key subjects including heat and mass transfers between phases, interfacial forces and fluid particles coalescence and breakup, a...

  18. Aqueous Biphasic Systems for the Synthesis of Formates by Catalytic CO2 Hydrogenation: Integrated Reaction and Catalyst Separation for CO2 -Scrubbing Solutions.

    Science.gov (United States)

    Scott, Martin; Blas Molinos, Beatriz; Westhues, Christian; Franciò, Giancarlo; Leitner, Walter

    2017-03-22

    Aqueous biphasic systems were investigated for the production of formate-amine adducts by metal-catalyzed CO2 hydrogenation, including typical scrubbing solutions as feedstocks. Different hydrophobic organic solvents and ionic liquids could be employed as the stationary phase for cis-[Ru(dppm)2 Cl2 ] (dppm=bis-diphenylphosphinomethane) as prototypical catalyst without any modification or tagging of the complex. The amines were found to partition between the two phases depending on their structure, whereas the formate-amine adducts were nearly quantitatively extracted into the aqueous phase, providing a favorable phase behavior for the envisaged integrated reaction/separation sequence. The solvent pair of methyl isobutyl carbinol (MIBC) and water led to the most practical and productive system and repeated use of the catalyst phase was demonstrated. The highest single batch activity with a TOFav of approximately 35 000 h(-1) and an initial TOF of approximately 180 000 h(-1) was achieved in the presence of NEt3 . Owing to higher stability, the highest productivities were obtained with methyl diethanolamine (Aminosol CST 115) and monoethanolamine (MEA), which are used in commercial scale CO2 -scrubbing processes. Saturated aqueous solutions (CO2 overpressure 5-10 bar) of MEA could be converted into the corresponding formate adducts with average turnover frequencies up to 14×10(3)  h(-1) with an overall yield of 70 % based on the amine, corresponding to a total turnover number of 150 000 over eleven recycling experiments. This opens the possibility for integrated approaches to carbon capture and utilization.

  19. Carbon nanohybrids used as catalysts and emulsifiers for reactions in biphasic aqueous/organic systems

    Institute of Scientific and Technical Information of China (English)

    Daniel E. Resasco

    2014-01-01

    This mini-review summarizes some novel aspects of reactions conducted in aqueous/organic emul-sions stabilized by carbon nanohybrids functionalized with catalytic species. Carbon nanohybrids represent a family of solid catalysts that not only can stabilize water-oil emulsions in the same fash-ion as Pickering emulsions, but also catalyze reactions at the liquid/liquid interface. Several exam-ples are discussed in this mini-review. They include (a) aldol condensation-hydrodeoxygenation tandem reactions catalyzed by basic (MgO) and metal (Pd) catalysts, respectively; (b) Fischer-Tropsch synthesis catalyzed by carbon-nanotube-supported Ru;and (c) emulsion polymerization of styrene for the production of conductive polymer composites. Conducting these reactions in emul-sion generates important advantages, such as increased liquid/liquid interfacial area that conse-quently means faster mass transfer rates of molecules between the two phases, effective separation of products from the reaction mixture by differences in the water-oil solubility, and significant changes in product selectivity that can be adjusted by modifying the emulsion characteristics.

  20. Shock wave of vapor-liquid two-phase flow

    Institute of Scientific and Technical Information of China (English)

    Liangju ZHAO; Fei WANG; Hong GAO; Jingwen TANG; Yuexiang YUAN

    2008-01-01

    The shock wave of vapor-liquid two-phase flow in a pressure-gain steam injector is studied by build-ing a mathematic model and making calculations. The results show that after the shock, the vapor is nearly com-pletely condensed. The upstream Mach number and the volume ratio of vapor have a great effect on the shock. The pressure and Mach number of two-phase shock con-form to the shock of ideal gas. The analysis of available energy shows that the shock is an irreversible process with entropy increase.

  1. Dynamic Modeling of Phase Crossings in Two-Phase Flow

    DEFF Research Database (Denmark)

    Madsen, Søren; Veje, Christian; Willatzen, Morten

    2012-01-01

    of the variables and are usually very slow to evaluate. To overcome these challenges, we use an interpolation scheme with local refinement. The simulations show that the method handles crossing of the saturation lines for both liquid to two-phase and two-phase to gas regions. Furthermore, a novel result obtained...... in this work, the method is stable towards dynamic transitions of the inlet/outlet boundaries across the saturation lines. Results for these cases are presented along with a numerical demonstration of conservation of mass under dynamically varying boundary conditions. Finally we present results...

  2. Two Phase Flow and Space-Based Applications

    Science.gov (United States)

    McQuillen, John

    1999-01-01

    A reduced gravity environment offers the ability to remove the effect of buoyancy on two phase flows whereby density differences that normally would promote relative velocities between the phases and also alter the shape of the interface are removed. However, besides being a potent research tool, there are also many space-based technologies that will either utilize or encounter two-phase flow behavior, and as a consequence, several questions must be addressed. This paper presents some of these technologies missions. Finally, this paper gives a description of web-sites for some funding.

  3. 独立光伏发电系统中双向变换器的软起动策略%Two-phase soft start strategy for bi-directional DC-DC converter in solar cell stand-alone photovoltaic power system

    Institute of Scientific and Technical Information of China (English)

    张新华; 廖志凌; 杨孟雄

    2009-01-01

    Bi-directional converter applied to solar cell stand-alone photovoltaic power system belongs to the double sources application. Due to complemented operation of switches in bi-directional converter, traditional soft start method will give rise to large inverse inductor current from the output source at double sources application, and result in damage of converter. This paper proposes a novel soft start method-two-phase soft start method to achieve soft start for bi-directional converter of double sources. By implementing soft start of both active switch and passive switch, two-phase soft start method can realize bi-directional converter soft start at double sources application, avoiding inverse inductor current. Two-phase soft start method applies to a series of bi-directional converter. Experimental results are finally given to verify the effectiveness and validity of two-phase soft start method for bi-directional converter of double sources.%双向变换器在太阳能独立光伏发电系统中属于两端"源"的运用场合,采用传统的软起动方法,由于功率开关管互补导通,会从输出端"源"引入反向电感电流,导致变换器损坏.为了实现双向变换器在两端"源"情况下的开机软起动,提出了一种新颖的双向变换器两段式软起动方法,通过对主控管和被控管实施软起动,避免产生反向电感电流,该方法也适用于一族的双向变换器.最后给出了实验结果,验证了两端"源"双向变换器两段式软起动方法的有效性和正确性.

  4. Multi-needle capacitance probe for non-conductive two-phase flows

    Science.gov (United States)

    Monrós-Andreu, G.; Martinez-Cuenca, R.; Torró, S.; Escrig, J.; Hewakandamby, B.; Chiva, S.

    2016-07-01

    Despite its variable degree of application, intrusive instrumentation is the most accurate way to obtain local information in a two-phase flow system, especially local interfacial velocity and local interfacial area parameters. In this way, multi-needle probes, based on conductivity or optical principles, have been extensively used in the past few decades by many researchers in two-phase flow investigations. Moreover, the signal processing methods used to obtain the time-averaged two-phase flow parameters in this type of sensor have been thoroughly discussed and validated by many experiments. The objective of the present study is to develop a miniaturized multi-needle probe, based on capacitance measurements applicable to a wide range of non-conductive two-phase flows and, thus, to extend the applicability of multi-needle sensor whilst also maintaining a signal processing methodology provided in the literature for conductivity probes. Results from the experiments performed assess the applicability of the proposed sensor measurement principle and signal processing method for the bubbly flow regime. These results also provide an insight into the sensor application for more complex two-phase flow regimes.

  5. Mineralogic controls on aqueous neptunium(V) concentrations in silicate systems

    Energy Technology Data Exchange (ETDEWEB)

    Alessi, Daniel S., E-mail: daniel.alessi@epfl.ch [Department of Civil Engineering and Geological Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Szymanowski, Jennifer E.S. [Department of Civil Engineering and Geological Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Forbes, Tori Z. [Department of Civil Engineering and Geological Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Department of Chemistry, University of Iowa, Room E331 CB, Iowa City, IA 52242 (United States); Quicksall, Andrew N. [Department of Civil Engineering and Geological Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Department of Civil and Environmental Engineering, Southern Methodist University, P.O. Box 750340, Dallas, TX 75275 (United States); Sigmon, Ginger E.; Burns, Peter C.; Fein, Jeremy B. [Department of Civil Engineering and Geological Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States)

    2013-02-15

    The presence of radioactive neptunium in commercially spent nuclear fuel is problematic due to its mobility in environmental systems upon oxidation to the pentavalent state. As uranium is the major component of spent fuel, incorporation of neptunium into resulting U(VI) mineral phases would potentially influence its release into environmental systems. Alternatively, aqueous neptunium concentrations may be buffered by solid phase Np{sub 2}O{sub 5}. In this study, we investigate both of these controls on aqueous neptunium(V) concentrations. We synthesize two uranyl silicates, soddyite, (UO{sub 2}){sub 2}SiO{sub 4}·2H{sub 2}O, and boltwoodite, (K, Na)(UO{sub 2})(SiO{sub 3}OH)·1.5H{sub 2}O, each in the presence of two concentrations of aqueous Np(V). Electron microscopy and electron diffraction analyses of the synthesized phases show that while significant neptunyl incorporation occurred into soddyite, the Np(V) in the boltwoodite systems largely precipitated as a secondary phase, Np{sub 2}O{sub 5(s)}. The release of Np(V) from each system into aqueous solution was measured for several days, until steady-state concentrations were achieved. Using existing solubility constants (K{sub sp}) for pure soddyite and boltwoodite, we compared predicted equilibrium aqueous U(VI) concentrations with the U(VI) concentrations released in the solubility experiments. Our experiments reveal that Np(V) incorporation into soddyite increases the concentration of aqueous U in equilibrium with the solid phase, perhaps via the formation of a metastable phase. In the mixed boltwoodite – Np{sub 2}O{sub 5(s)} system, the measured aqueous U(VI) activities are consistent with those predicted to be in equilibrium with boltwoodite under the experimental conditions, a result that is consistent with our conclusion that little Np(V) incorporation occurred into the boltwoodite. In the boltwoodite systems, the measured Np concentrations are likely controlled by the presence of Np{sub 2}O{sub 5

  6. Entropy analysis on non-equilibrium two-phase flow models

    Energy Technology Data Exchange (ETDEWEB)

    Karwat, H.; Ruan, Y.Q. [Technische Universitaet Muenchen, Garching (Germany)

    1995-09-01

    A method of entropy analysis according to the second law of thermodynamics is proposed for the assessment of a class of practical non-equilibrium two-phase flow models. Entropy conditions are derived directly from a local instantaneous formulation for an arbitrary control volume of a structural two-phase fluid, which are finally expressed in terms of the averaged thermodynamic independent variables and their time derivatives as well as the boundary conditions for the volume. On the basis of a widely used thermal-hydraulic system code it is demonstrated with practical examples that entropy production rates in control volumes can be numerically quantified by using the data from the output data files. Entropy analysis using the proposed method is useful in identifying some potential problems in two-phase flow models and predictions as well as in studying the effects of some free parameters in closure relationships.

  7. Analytical solution of laminar-laminar stratified two-phase flows with curved interfaces

    Energy Technology Data Exchange (ETDEWEB)

    Brauner, N.; Rovinsky, J.; Maron, D.M. [Tel-Aviv Univ. (Israel)

    1995-09-01

    The present study represents a complete analytical solution for laminar two-phase flows with curved interfaces. The solution of the Navier-Stokes equations for the two-phases in bipolar coordinates provides the `flow monograms` describe the relation between the interface curvature and the insitu flow geometry when given the phases flow rates and viscosity ratios. Energy considerations are employed to construct the `interface monograms`, whereby the characteristic interfacial curvature is determined in terms of the phases insitu holdup, pipe diameter, surface tension, fluids/wall adhesion and gravitation. The two monograms are then combined to construct the system `operational monogram`. The `operational monogram` enables the determination of the interface configuration, the local flow characteristics, such as velocity profiles, wall and interfacial shear stresses distribution as well as the integral characteristics of the two-phase flow: phases insitu holdup and pressure drop.

  8. Investigation of two-phase heat transfer coefficients of argon-freon cryogenic mixed refrigerants

    Science.gov (United States)

    Baek, Seungwhan; Lee, Cheonkyu; Jeong, Sangkwon

    2014-11-01

    Mixed refrigerant Joule Thomson refrigerators are widely used in various kinds of cryogenic systems these days. Although heat transfer coefficient estimation for a multi-phase and multi-component fluid in the cryogenic temperature range is necessarily required in the heat exchanger design of mixed refrigerant Joule Thomson refrigerators, it has been rarely discussed so far. In this paper, condensation and evaporation heat transfer coefficients of argon-freon mixed refrigerant are measured in a microchannel heat exchanger. A Printed Circuit Heat Exchanger (PCHE) with 340 μm hydraulic diameter has been developed as a compact microchannel heat exchanger and utilized in the experiment. Several two-phase heat transfer coefficient correlations are examined to discuss the experimental measurement results. The result of this paper shows that cryogenic two-phase mixed refrigerant heat transfer coefficients can be estimated by conventional two-phase heat transfer coefficient correlations.

  9. Research of Characteristics of Gas-liquid Two-phase Pressure Drop in Microreactor

    OpenAIRE

    Li Dan

    2015-01-01

    With the research system of nitrogen and deionized water, this paper researches the pressure drop of gas-liquid two-phase flow in the circular microchannel with an inner diameter which is respectively 0.9mm and 0.5mm, analyzes the effect of microchannel diameter on gas-liquid two-phase frictional pressure drop in the microchannel reactor, and compares with the result of frictional pressure drop and the predicting result of divided-phase flow pattern. The result shows that, the gas-liquid two-...

  10. Problems of heat transfer and hydraulics of two-phase media

    CERN Document Server

    Kutateladze, S S

    1969-01-01

    Problems of Heat Transfer and Hydraulics of Two-Phase Media presents the theory of heat transfer and hydrodynamics. This book discusses the various aspects of heat transfer and the flow of two-phase systems. Organized into two parts encompassing 22 chapters, this book starts with an overview of the laws of similarity for heat transfer to or from a flowing liquid with various physical properties and allowed for variation in viscosity and thermal conductivity. This book then explores the general functional relationship that exists between viscosity and thermal conductivity for thermodynamically

  11. Position Control of Synchronous Motor Drive by Modified Adaptive Two-phase Sliding Mode Controller

    Institute of Scientific and Technical Information of China (English)

    Mohamed Said Sayed Ahmed; Ping Zhang; Yun-Jie Wu

    2008-01-01

    A modified adaptive two-phase sliding mode controller for the synchronous motor drive that is highly robust to uncertain-ties and external disturbances is proposed in this paper. The proposed controller uses two-phase sliding mode control (SMC) where the 1st phase mainly controls the system in steady states and disturbed states-it is a smoothing phase. The 2nd phase is used mainly in the case of disturbed states. Also, it is an autotuning phase and uses a simple adaptive algorithm to tune the gain of conventional variable structure control (VSC). The modified controller is useful in position control of a permanent magnet synchronous drive.

  12. TWO-PHASE EJECTOR of CARBON DIOXIDE HEAT PUMP CALCULUS

    Directory of Open Access Journals (Sweden)

    Sit B.M.

    2010-12-01

    Full Text Available It is presented the calculus of the two-phase ejector for carbon dioxide heat pump. The method of calculus is based on the method elaborated by S.M. Kandil, W.E. Lear, S.A. Sherif, and is modified taking into account entrainment ratio as the input for the calculus.

  13. Numerical simulation of two-phase flow in offshore environments

    NARCIS (Netherlands)

    Wemmenhove, Rik

    2008-01-01

    Numerical Simulation of Two-Phase Flow in Offshore Environments Rik Wemmenhove Weather conditions on full sea are often violent, leading to breaking waves and lots of spray and air bubbles. As high and steep waves may lead to severe damage on ships and offshore structures, there is a great need for

  14. Two-phase alkali-metal experiments in reduced gravity

    Energy Technology Data Exchange (ETDEWEB)

    Antoniak, Z.I.

    1986-06-01

    Future space missions envision the use of large nuclear reactors utilizing either a single or a two-phase alkali-metal working fluid. The design and analysis of such reactors require state-of-the-art computer codes that can properly treat alkali-metal flow and heat transfer in a reduced-gravity environment. A literature search of relevant experiments in reduced gravity is reported on here, and reveals a paucity of data for such correlations. The few ongoing experiments in reduced gravity are noted. General plans are put forth for the reduced-gravity experiments which will have to be performed, at NASA facilities, with benign fluids. A similar situation exists regarding two-phase alkali-metal flow and heat transfer, even in normal gravity. Existing data are conflicting and indequate for the task of modeling a space reactor using a two-phase alkali-metal coolant. The major features of past experiments are described here. Data from the reduced-gravity experiments with innocuous fluids are to be combined with normal gravity data from the two-phase alkali-metal experiments. Analyses undertaken here give every expectation that the correlations developed from this data base will provide a valid representation of alkali-metal heat transfer and pressure drop in reduced gravity.

  15. Coal-Face Fracture With A Two-Phase Liquid

    Science.gov (United States)

    Collins, E. R., Jr.

    1985-01-01

    In new method for mining coal without explosive, two-phase liquid such as CO2 and water, injected at high pressure into deeper ends of holes drilled in coal face. Liquid permeates coal seam through existing microfractures; as liquid seeps back toward face, pressure eventually drops below critical value at which dissolved gas flashvaporizes, breaking up coal.

  16. Two-phase flow in micro and nanofluidic devices

    NARCIS (Netherlands)

    Shui, Lingling

    2009-01-01

    This thesis provides experimental data and theoretical analysis on two-phase flow in devices with different layouts of micrometer or nanometer-size channels. A full flow diagram is presented for oil and water flow in head-on microfluidic devices. Morphologically different flow regimes (dripping, jet

  17. Modelling two-phase transport of 3H/3He

    NARCIS (Netherlands)

    Visser, A.; Schaap, J.D.; Leijnse, T.; Broers, H.P.; Bierkens, M.F.P.

    2008-01-01

    Degassing of groundwater by excess denitrification of agricultural pollution complicates the interpretation of 3H/3He data and hinders the estimation of travel times in nitrate pollution studies. In this study we used a two-phase flow and transport model (STOMP) to evaluate the method presented by V

  18. Radiation effects on hydroxypropyl methylcellulose phthalate in aqueous system

    Energy Technology Data Exchange (ETDEWEB)

    Xu Ling; Yue Zhiying; Wang Min [Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Zhai Maolin [Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)], E-mail: mlzhai@pku.edu.cn; Yoshii, Fumio; Seko, Noriaki [Environment and Industrial Materials Research Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki, Takasaki 370-1292 (Japan); Peng Jing; Wei Genshuan; Li Jiuqiang [Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China)

    2007-12-15

    A water-insoluble cellulose derivative, hydroxypropyl methylcellulose phthalate (HPMCP) hydrogels, was converted to Na type to form hydrogel in paste-like status by radiation crosslinking. Mechanism for radiation crosslinking of cellulose-derivatives in paste-like status was discussed. Crosslinkers, i.e. methyl N,N-bis-acrylamide (MBA) or ethyleneglycol dimethacrylate (EGDMA) has been used to decrease gelation dose (Dg) of synthesis HPMCP hydrogels and improve its mechanical properties. HPMCP-MBA hydrogels were found to be more rigid and HPMCP-EGDMA hydrogels were more flexible. Swelling degree of HPMCP hydrogel in many kinds of salt solutions followed Hofmeister series, which is ubiquitous in polyelectrolyte hydrogel. Specific reswelling was observed in concentrated KF solution, implying a very strong F{sup -} binding ability of benzyl group. The comprehensive results obtained in this study will be utilized on the design of HPMCP-based controlled release system.

  19. Radiation effects on hydroxypropyl methylcellulose phthalate in aqueous system

    Science.gov (United States)

    Xu, Ling; Yue, Zhiying; Wang, Min; Zhai, Maolin; Yoshii, Fumio; Seko, Noriaki; Peng, Jing; Wei, Genshuan; Li, Jiuqiang

    2007-12-01

    A water-insoluble cellulose derivative, hydroxypropyl methylcellulose phthalate (HPMCP) hydrogels, was converted to Na type to form hydrogel in paste-like status by radiation crosslinking. Mechanism for radiation crosslinking of cellulose-derivatives in paste-like status was discussed. Crosslinkers, i.e. methyl N, N-bis-acrylamide (MBA) or ethyleneglycol dimethacrylate (EGDMA) has been used to decrease gelation dose (Dg) of synthesis HPMCP hydrogels and improve its mechanical properties. HPMCP-MBA hydrogels were found to be more rigid and HPMCP-EGDMA hydrogels were more flexible. Swelling degree of HPMCP hydrogel in many kinds of salt solutions followed Hofmeister series, which is ubiquitous in polyelectrolyte hydrogel. Specific reswelling was observed in concentrated KF solution, implying a very strong F - binding ability of benzyl group. The comprehensive results obtained in this study will be utilized on the design of HPMCP-based controlled release system.

  20. THE LINEAR HOMOGENEOUS FLOW MODEL FOR TWO-PHASE FLOW INSTABILITY IN BOILING CHANNELS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    This paper presents liner homogeneous model describing two-phase flow instability. Dimensionless parameter η was derived by using the linear homogeneous model. Using parameter η the stability of a system could be easily judged. The calculated results agree with the experimental data well.

  1. Generating a Two-Phase Lesson for Guiding Beginners to Learn Basic Dance Movements

    Science.gov (United States)

    Yang, Yang; Leung, Howard; Yue, Lihua; Deng, Liqun

    2013-01-01

    In this paper, an automated lesson generation system for guiding beginners to learn basic dance movements is proposed. It analyzes the dance to generate a two-phase lesson which can provide a suitable cognitive load thus offering an efficient learning experience. In the first phase, the dance is divided into small pieces which are patterns, and…

  2. Measurements of solids concentration and axial solids velocity in gas-solid two-phase flows.

    NARCIS (Netherlands)

    Nieuwland, J.J.; Meijer, R.; Kuipers, J.A.M.; Swaaij, van W.P.M.

    1996-01-01

    Several techniques reported in the literature for measuring solids concentration and solids velocity in (dense) gas-solid two-phase flow have been briefly reviewed. An optical measuring system, based on detection of light reflected by the suspended particles, has been developed to measure local soli

  3. A FINITE ELEMENT COLLOCATION METHOD FOR TWO-PHASE INCOMPRESSIBLE IMMISCIBLE PROBLEMS

    Institute of Scientific and Technical Information of China (English)

    Ma Ning

    2007-01-01

    Two-phase, incompressible, immiscible flow in porous media is governed by a coupled system of nonlinear partial differential equations. The pressure equation is elliptic,whereas the concentration equation is parabolic, and both are treated by the collocation scheme. Existence and uniqueness of solutions of the algorithm are proved. A optimal convergence analysis is given for the method.

  4. The treatment of gaseous benzene by two-phase partitioning bioreactors: a high performance alternative to the use of biofilters

    Energy Technology Data Exchange (ETDEWEB)

    Davidson, C.T.; Daugulis, A.J. [Dept. of Chemical Engineering, Queen' s Univ., Kingston, ON (Canada)

    2003-07-01

    A 2-1 (1-1 working volume) two-phase partitioning bioreactor (TPPB) was used as an integrated scrubber/bioreactor in which the removal and destruction of benzene from a gas stream was achieved by the reactor's organic/aqueous liquid contents. The organic solvent used to trap benzene was n-hexadecane, and degradation of benzene was achieved in the aqueous phase using the bacterium Alcaligenes xylosoxidans Y234. A gas stream with a benzene concentration of 340 mg l{sup -1} at a flow rate of 0.414 l h{sup -1} was delivered to the system at a loading capacity of 140 g m{sup -3} h{sup -1}, and an elimination capacity of 133 g m{sup -3} h{sup -1} was achieved (the volume in this term is the total liquid volume of the TPPB). This elimination capacity is between 3 and 13 times greater than any benzene elimination achieved by biofiltration, a competing biological air treatment strategy. It was also determined that the evaluation of TPPB performance in terms of elimination capacity should include the cell mass present in the system, as this is a readily controllable quantity. A specific benzene utilization rate of 0.57 g benzene (g cells){sup -1} h{sup -1} was experimentally determined in a bioreactor with a cell concentration that varied dynamically between 0.2 and 1 g l{sup -1}. If it assumed that this specific benzene utilization rate (0.57 g g{sup -1} h{sup -1}) is independent of cell concentration, then a TPPB operated at high cell concentrations could potentially achieve elimination capacities several hundred times greater than those obtained with biofilters. (orig.)

  5. Cyclic electrowinning/precipitation (CEP) system for the removal of heavy metal mixtures from aqueous solutions.

    Science.gov (United States)

    Grimshaw, Pengpeng; Calo, Joseph M; Hradil, George

    2011-11-15

    The description and operation of a novel cyclic electrowinning/precipitation (CEP) system for the simultaneous removal of mixtures of heavy metals from aqueous solutions are presented. CEP combines the advantages of electrowinning in a spouted particulate electrode (SPE) with that of chemical precipitation and redissolution, to remove heavy metals at low concentrations as solid metal deposits on particulate cathode particles without exporting toxic metal precipitate sludges from the process. The overall result is very large volume reduction of the heavy metal contaminants as a solid metal deposit on particles that can either be safely discarded as such, or further processed to recover particular metals. The performance of this system is demonstrated with data on the removal of mixtures of copper, nickel, and cadmium from aqueous solutions.

  6. Investigations of two-phase flame propagation under microgravity conditions

    Science.gov (United States)

    Gokalp, Iskender

    2016-07-01

    Investigations of two-phase flame propagation under microgravity conditions R. Thimothée, C. Chauveau, F. Halter, I Gökalp Institut de Combustion, Aérothermique, Réactivité et Environnement (ICARE), CNRS, 1C Avenue de la Recherche Scientifique, 45071 Orléans Cedex 2, France This paper presents and discusses recent results on two-phase flame propagation experiments we carried out with mono-sized ethanol droplet aerosols under microgravity conditions. Fundamental studies on the flame propagation in fuel droplet clouds or sprays are essential for a better understanding of the combustion processes in many practical applications including internal combustion engines for cars, modern aircraft and liquid rocket engines. Compared to homogeneous gas phase combustion, the presence of a liquid phase considerably complicates the physico-chemical processes that make up combustion phenomena by coupling liquid atomization, droplet vaporization, mixing and heterogeneous combustion processes giving rise to various combustion regimes where ignition problems and flame instabilities become crucial to understand and control. Almost all applications of spray combustion occur under high pressure conditions. When a high pressure two-phase flame propagation is investigated under normal gravity conditions, sedimentation effects and strong buoyancy flows complicate the picture by inducing additional phenomena and obscuring the proper effect of the presence of the liquid droplets on flame propagation compared to gas phase flame propagation. Conducting such experiments under reduced gravity conditions is therefore helpful for the fundamental understanding of two-phase combustion. We are considering spherically propagating two-phase flames where the fuel aerosol is generated from a gaseous air-fuel mixture using the condensation technique of expansion cooling, based on the Wilson cloud chamber principle. This technique is widely recognized to create well-defined mono-size droplets

  7. The effects of aqueous extracts of Desmodium gangeticum DC. (Leguminosae) on the central nervous system.

    Science.gov (United States)

    Jabbar, S; Khan, M T; Choudhuri, M S

    2001-06-01

    The aqueous extract of Desmodium gangeticum DC. (Leguminosae) showed no analgesic activity in the hot plate method, but it showed severe anti-writhing activity in the acetic acid-induced abdominal writhing assay. It exhibited moderate central nervous system depressant activity in the spontaneous motor activity, hole cross, and open field tests and hole board tests. The effects of this extract on locomotion were compared with some standard CNS drugs.

  8. Dehalogenation of Aryl Halides Catalyzed by Montmorillonite Immobilized Bimetal Catalyst in Aqueous System

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A novel bisupported bimetal catalyst PVP-PdCl2-FeSO4/Al-Mont-PEG600 was prepared by immobilization of PVP (poly (N-vinyl-2-pyrrolidone)) supported bimetallic catalyst using alumina pillared inartificial montmorillonite as the carrier. This catalyst has good dehalogenation activity and selectivity to aryl halides-o-chlorotoluene in aqueous system in the presence of phase transfer catalyst (PEG) and sodium formate as hydrogen source. The catalyst also shows good reusability.

  9. Computer simulation of two-phase flow in nuclear reactors

    Energy Technology Data Exchange (ETDEWEB)

    Wulff, W.

    1992-09-01

    Two-phase flow models dominate the economic resource requirements for development and use of computer codes for analyzing thermohydraulic transients in nuclear power plants. Six principles are presented on mathematical modeling and selection of numerical methods, along with suggestions on programming and machine selection, all aimed at reducing the cost of analysis. Computer simulation is contrasted with traditional computer calculation. The advantages of run-time interactive access operation in a simulation environment are demonstrated. It is explained that the drift-flux model is better suited for two-phase flow analysis in nuclear reactors than the two-fluid model, because of the latter`s closure problem. The advantage of analytical over numerical integration is demonstrated. Modeling and programming techniques are presented which minimize the number of needed arithmetical and logical operations and thereby increase the simulation speed, while decreasing the cost.

  10. Computer simulation of two-phase flow in nuclear reactors

    Energy Technology Data Exchange (ETDEWEB)

    Wulff, W.

    1992-01-01

    Two-phase flow models dominate the economic resource requirements for development and use of computer codes for analyzing thermohydraulic transients in nuclear power plants. Six principles are presented on mathematical modeling and selection of numerical methods, along with suggestions on programming and machine selection, all aimed at reducing the cost of analysis. Computer simulation is contrasted with traditional computer calculation. The advantages of run-time interactive access operation in a simulation environment are demonstrated. It is explained that the drift-flux model is better suited for two-phase flow analysis in nuclear reactors than the two-fluid model, because of the latter's closure problem. The advantage of analytical over numerical integration is demonstrated. Modeling and programming techniques are presented which minimize the number of needed arithmetical and logical operations and thereby increase the simulation speed, while decreasing the cost.

  11. Two-dimensional Rarefaction Waves in the High-speed Two-phase Flow

    Science.gov (United States)

    Nakagawa, Masafumi; Harada, Atsushi

    Two-phase flow nozzles are used in the total flow system for geothermal power plants and in the ejector of the refrigerant cycle, etc. One of the most important functions of a two-phase flow nozzle is to convert the thermal energy to the kinetic energy of the two-phase flow. The kinetic energy of the two-phase flow exhausted from a nozzle is available for all applications of this type. There exist the shock waves or rarefaction waves at the outlet of a supersonic nozzle in the case of non-best fitting expansion conditions when the operation conditions of the nozzle are widely chosen. The purpose of the present study is to elucidate theoretically the character of the rarefaction waves at the outlet of the supersonic two-phase flow nozzle. Two-dimensional basic equations for the compressible two-phase flow are introduced considering the inter-phase momentum transfer. Sound velocities are obtained from these equations by using monochromatic wave approximation. Those depend on the relaxation time that determines the momentum transfer. The two-phase flow with large relaxation times has a frozen sound velocity, and with small one has an equilibrium sound velocity. Rarefaction waves which occurred behind the two-phase flow nozzle are calculated by the CIP method. Although the frozen Mach number, below one, controls these basic equations, the rarefaction waves appeared for small relaxation time. The Mach line behind which the expansion starts depends on the inlet velocity and the relaxation time. Those relationships are shown in this paper. The pressure expansion curves are only a function of the revolution angle around the corner of the nozzle outlet for the relaxation time less than 0.1. For the larger relaxation time, the pressure decays because of internal friction caused by inter phase momentum transfer, and the expansion curves are a function of not only the angle but also the flow direction. The calculated expansion curves are compared with the experimental ones

  12. Recent advances in two-phase flow numerics

    Energy Technology Data Exchange (ETDEWEB)

    Mahaffy, J.H.; Macian, R. [Pennsylvania State Univ., University Park, PA (United States)

    1997-07-01

    The authors review three topics in the broad field of numerical methods that may be of interest to individuals modeling two-phase flow in nuclear power plants. The first topic is iterative solution of linear equations created during the solution of finite volume equations. The second is numerical tracking of macroscopic liquid interfaces. The final area surveyed is the use of higher spatial difference techniques.

  13. Viscosity Solutions for the two-phase Stefan Problem

    CERN Document Server

    Kim, Inwon C

    2010-01-01

    We introduce a notion of viscosity solutions for the two-phase Stefan problem, which incorporates possible existence of a mushy region generated by the initial data. We show that a comparison principle holds between viscosity solutions, and investigate the coincidence of the viscosity solutions and the weak solutions defined via integration by parts. In particular, in the absence of initial mushy region, viscosity solution is the unique weak solution with the same boundary data.

  14. Computational methods for two-phase flow and particle transport

    CERN Document Server

    Lee, Wen Ho

    2013-01-01

    This book describes mathematical formulations and computational methods for solving two-phase flow problems with a computer code that calculates thermal hydraulic problems related to light water and fast breeder reactors. The physical model also handles the particle and gas flow problems that arise from coal gasification and fluidized beds. The second part of this book deals with the computational methods for particle transport.

  15. Two-phase Flow Distribution in Heat Exchanger Manifolds

    OpenAIRE

    Vist, Sivert

    2004-01-01

    The current study has investigated two-phase refrigerant flow distribution in heat exchange manifolds. Experimental data have been acquired in a heat exchanger test rig specially made for measurement of mass flow rate and gas and liquid distribution in the manifolds of compact heat exchangers. Twelve different manifold designs were used in the experiments, and CO2 and HFC-134a were used as refrigerants.

  16. Multi-scale symbolic time reverse analysis of gas-liquid two-phase flow structures

    Science.gov (United States)

    Wang, Hongmei; Zhai, Lusheng; Jin, Ningde; Wang, Youchen

    Gas-liquid two-phase flows are widely encountered in production processes of petroleum and chemical industry. Understanding the dynamic characteristics of multi-scale gas-liquid two-phase flow structures is of great significance for the optimization of production process and the measurement of flow parameters. In this paper, we propose a method of multi-scale symbolic time reverse (MSTR) analysis for gas-liquid two-phase flows. First, through extracting four time reverse asymmetry measures (TRAMs), i.e. Euclidean distance, difference entropy, percentage of constant words and percentage of reversible words, the time reverse asymmetry (TRA) behaviors of typical nonlinear systems are investigated from the perspective of multi-scale analysis, and the results show that the TRAMs are sensitive to the changing of dynamic characteristics underlying the complex nonlinear systems. Then, the MSTR analysis is used to study the conductance signals from gas-liquid two-phase flows. It is found that the multi-scale TRA analysis can effectively reveal the multi-scale structure characteristics and nonlinear evolution properties of the flow structures.

  17. An ALE Finite Element Approach for Two-Phase Flow with Phase Change

    Science.gov (United States)

    Gros, Erik; Anjos, Gustavo; Thome, John; Ltcm Team; Gesar Team

    2016-11-01

    In this work, two-phase flow with phase change is investigated through the Finite Element Method (FEM) in the Arbitrary Lagrangian-Eulerian (ALE) framework. The equations are discretized on an unstructured mesh where the interface between the phases is explicitly defined as a sub-set of the mesh. The two-phase interface position is described by a set of interconnected nodes which ensures a sharp representation of the boundary, including the role of the surface tension. The methodology proposed for computing the curvature leads to very accurate results with moderate programming effort and computational costs. Such a methodology can be employed to study accurately many two-phase flow and heat transfer problems in industry such as oil extraction and refinement, design of refrigeration systems, modelling of microfluidic and biological systems and efficient cooling of electronics for computational purposes. The latter is the principal aim of the present research. The numerical results are discussed and compared to analytical solutions and reference results, thereby revealing the capability of the proposed methodology as a platform for the study of two-phase flow with phase change.

  18. Estimating disease prevalence in two-phase studies.

    Science.gov (United States)

    Alonzo, Todd A; Pepe, Margaret Sullivan; Lumley, Thomas

    2003-04-01

    Disease prevalence is ideally estimated using a 'gold standard' to ascertain true disease status on all subjects in a population of interest. In practice, however, the gold standard may be too costly or invasive to be applied to all subjects, in which case a two-phase design is often employed. Phase 1 data consisting of inexpensive and non-invasive screening tests on all study subjects are used to determine the subjects that receive the gold standard in the second phase. Naive estimates of prevalence in two-phase studies can be biased (verification bias). Imputation and re-weighting estimators are often used to avoid this bias. We contrast the forms and attributes of the various prevalence estimators. Distribution theory and simulation studies are used to investigate their bias and efficiency. We conclude that the semiparametric efficient approach is the preferred method for prevalence estimation in two-phase studies. It is more robust and comparable in its efficiency to imputation and other re-weighting estimators. It is also easy to implement. We use this approach to examine the prevalence of depression in adolescents with data from the Great Smoky Mountain Study.

  19. Cytotoxicity of aqueous extracts of Rosmarinus officinalis L. (Labiatae in plant test system

    Directory of Open Access Journals (Sweden)

    GHS Cardoso

    Full Text Available This study investigated the cytotoxic activity of Rosmarinus officinalis L. (rosemary aqueous extract on the cell cycle of Allium cepa. To this end, crude aqueous leaf extracts at four concentrations, 0.02, 0.04, 0.06 and 0.08 mg/mL, were tested on A. cepa meristematic root cells, at exposure times of 24 and 48h. Slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5,000 for each control group and concentration. The four concentrations tested, including the lowest and considered ideal for use, at all exposure times, showed a significant antiproliferative effect on the cell cycle of this test system and presented a high number of cells in prophase. Our results evidenced the cytotoxicity of rosemary extracts, under the studied conditions.

  20. Cytotoxicity of aqueous extracts of Rosmarinus officinalis L. (Labiatae) in plant test system.

    Science.gov (United States)

    Cardoso, G H S; Dantas, E B S; Sousa, F R C; Peron, A P

    2014-11-01

    This study investigated the cytotoxic activity of Rosmarinus officinalis L. (rosemary) aqueous extract on the cell cycle of Allium cepa. To this end, crude aqueous leaf extracts at four concentrations, 0.02, 0.04, 0.06 and 0.08 mg/mL, were tested on A. cepa meristematic root cells, at exposure times of 24 and 48 h. Slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5,000 for each control group and concentration. The four concentrations tested, including the lowest and considered ideal for use, at all exposure times, showed a significant antiproliferative effect on the cell cycle of this test system and presented a high number of cells in prophase. Our results evidenced the cytotoxicity of rosemary extracts, under the studied conditions.